LOCUS       DAZKLM010000001        80143 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3764
            SAMEA115706913-rid22582563.denovo.001, whole genome shotgun
            sequence.
ACCESSION   DAZKLM010000001 DAZKLM010000000
VERSION     DAZKLM010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706913
            Sequence Read Archive: ERR13196647
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 80143)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 80143)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287823.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287823.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,004
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,849
            CDSs (with protein)               :: 2,849
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 41 of 96
            Pseudo Genes (incomplete)         :: 52 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 15 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..80143
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706913-rid22582563.denovo.001"
                     /isolate="VRE3764"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(218..958)
                     /locus_tag="ACG842_000001"
     CDS             complement(218..958)
                     /locus_tag="ACG842_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0246574.1"
                     /translation="MKEIRRKHFRFPAYDDELGVKLTNDKKRVLFDGQEDLLTEQNQS
                     PFEQMKEFHFTNTNTEKIRRKRRATNGLKSAPRKENLEKHKGKLPDYTGHYQSEITST
                     GRKKLFGDSSRSVRQSETRTESKHTTRNASVDTKVRERSYFVPKYVPASIIPDPRESK
                     ISEDHLIESMKKTDDSYLLFDTEPTPYQEKRNGEPSVRTFNRTKTLEETKQSEEKKSK
                     KSHGILERSLQGLIDDQGNSLGENSYFK"
     gene            complement(1088..2035)
                     /locus_tag="ACG842_000002"
     CDS             complement(1088..2035)
                     /locus_tag="ACG842_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFQ0246575.1"
                     /translation="MPTLADVAKRANVSKMTVSRVINHPEQVTDELKELVFSAMAELD
                     YRPNIAAKALVTNRSQIIKLFILEEIDTTEPYYMNLLMGIAKRIGKAHYSLQLVTNDA
                     FDVGSCDGYIITGVRKKDFDWIDRLEKPVVLFGENSEGYDYVDSDNEYGTAKATQYAL
                     ERDYQTIIYIGIEVDEPFEISRETGYLSVMKEVDLPSKILRFPNHSTEVERYMTENWA
                     DFTDKTCFVCSSDRLALGAVRGIINAGGHLPEEFGVIGFDGVFLDQISSPKLTTIKQD
                     IIRMGEVCGEMLLKKIEEKGASQGYRRFFPELVIRETTN"
     gene            complement(2164..2928)
                     /locus_tag="ACG842_000003"
     CDS             complement(2164..2928)
                     /locus_tag="ACG842_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFQ0246576.1"
                     /translation="MNPIILKRSVHHIPVLEVVLKELREERIPVVIYYHGWQTSKELV
                     LTQGRKIAQQGIRVILPDAMNHGERKQPVSSIPSYTFWDSIYGNLFEFDTLIKHLEKR
                     GLLHDQLAVGGVSMGGMTTCALLAKHPHIVAGVCLMGSPAPLEYGKEIVRRASEYNYV
                     VPKDYFDLISWVKSYDLSLQPEKLAGRPLFFWHGQADEKIPYIQTERFVEKNRHKSYG
                     KEIVFRSSKTQRHMVQIPLMEEASTFLAKNLIKKYK"
     gene            complement(2932..3321)
                     /locus_tag="ACG842_000004"
     CDS             complement(2932..3321)
                     /locus_tag="ACG842_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0246577.1"
                     /translation="MKTVNRVFCASHVLSLLLSIYLGVYSIQQPIEVENIVFQMSLTD
                     GLILSVFFFLILFIGNIFGSVASFLNFSIYPLLSLALGVVGLCSLCLFPEPFSPAFIL
                     FGTLNLFQTTVGAWLLWRSGNLMKIGE"
     gene            3456..3998
                     /locus_tag="ACG842_000005"
     CDS             3456..3998
                     /locus_tag="ACG842_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HFQ0246578.1"
                     /translation="MIDPKMLGMIFLINFAYITLNTLRFMLTMKGYRLIAPLVSMVEI
                     TIYIVGLSLVLDRLDNPLNLFVYALGYAVGISVGILIEDKLALGYIMVTTILPANSAE
                     DKSLPGILREHGYGVTQTSAMGLEGERLVLEILSPRKSERELYRLIKEIEERAFIISY
                     EPKYISGGFWTKKVRKRQGK"
     gene            4146..4778
                     /locus_tag="ACG842_000006"
     CDS             4146..4778
                     /locus_tag="ACG842_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0246579.1"
                     /translation="MEIQRRTTNFLHLGSFFATLTILFFLCYSYLKTSHAPSSALGWL
                     LLDHSDITEAEENPFFLILSSLCGLLFSVYFGAYFFHRHPGTLNEASSVLIAFKTFSF
                     LAILFYSFNHNTLFFFIMNGLVVILSIFTYFIFSLIFSQLKCPLFFRLVPVTCFIYSL
                     FSFFVLALMPLTNPSTIQVAGNLLDMLFIVSLGVFMWQRKNKQNKKHFEA"
     gene            complement(4912..5037)
                     /locus_tag="ACG842_000007"
     CDS             complement(4912..5037)
                     /locus_tag="ACG842_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HFQ0246580.1"
                     /translation="MQKNGEKCGMTKEVVIRKVRFLNNQYYDSVKYGILWEELAD"
     gene            complement(5171..6064)
                     /locus_tag="ACG842_000008"
     CDS             complement(5171..6064)
                     /locus_tag="ACG842_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1803 domain-containing protein"
                     /protein_id="HFQ0246581.1"
                     /translation="MNYYSKNKENFYQKLTDDPLFSSLTDYLYEHREQETILRELKKE
                     FSQNKFSHFLDLLIDAGLIKREERRYHLNFPIFDPKDYLQQATSAAETIAEQLKRLSV
                     DEQKLAMGEVIWAYCFEDERKEAYFYGVRNSRETELLRTTAGNEKYRFITLSSIEHFP
                     LTLANYFFVQKNQLPVTKAFKELAELIGDVNESYFFDQIEVIVDRIRKNKYKNRRPSI
                     FHQSLLVTNTIKEEESFTLELPIVEKNNFEIELPTLDPSLTMEEIAFLKRQIFSELSK
                     KFIPHAFSYIKEYGTVLVSKT"
     gene            complement(6246..7172)
                     /locus_tag="ACG842_000009"
     CDS             complement(6246..7172)
                     /locus_tag="ACG842_000009"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HFQ0246582.1"
                     /translation="MSKILVFGHQNPDTDAIGAAIAFSHLQNELGKETEAVALGTPNE
                     ETQYALDHFQLSAPRVVESAKAENADVMLVDHNEFQQSISDISEVNILAVVDHHRIAN
                     FETANPLYYRAEPVGCTSTIILKLYKENQVTIPKEIAGIMLSAIISDTLLFKSPTCTP
                     EDEAAAKELAAIAEVDLNTYGLEMLKAGTNLSDKSAAVLLDLDAKSFPMGSSNVRIAQ
                     VNTVDLNEVMDRQAELEAAMEDEMKANGYDLFVLVVTNILDSDSELLVAGKGKEKVEE
                     AFQTTLTNNRALLKGVVSRKKQVVPQLTAVFS"
     gene            complement(7257..8018)
                     /gene="pflA"
                     /locus_tag="ACG842_000010"
     CDS             complement(7257..8018)
                     /gene="pflA"
                     /locus_tag="ACG842_000010"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate-lyase-activating protein"
                     /protein_id="HFQ0246583.1"
                     /translation="MEEKTIGYVHSIETFGSVDGPGLRFVVFMQGCRMRCQFCHNPDT
                     WNIGGGKEYTADELLDKAERFRPYWGDKGGITVSGGEPLLQIDFLIELFKKAKERKMH
                     TTLDTCGKPFTYEEPFFSRFQELMKYTDLLLFDIKHIDNEEHKKLTHWDNENILEMAQ
                     YLSKINKPVWIRHVLVPERSDYDEYLIRLDNFIQTLSNVDRVEILPYHTMGKYKWETL
                     GLKYPLEGIEPPTKERVENAKRLLHTEEYTKYLTR"
     gene            complement(8262..10493)
                     /gene="pflB"
                     /locus_tag="ACG842_000011"
     CDS             complement(8262..10493)
                     /gene="pflB"
                     /locus_tag="ACG842_000011"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008861 - formate C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HFQ0246584.1"
                     /translation="MEQWNGFKGTKWKEGVDTRDFIQNNYTEYRGDDSFLEGPTEATE
                     KLWDELQKLNDIQFERNGVYDMDTEIVSTITSHDAGYLDKDLEKIVGLQTDKPLKQAF
                     MPFGGINMANNALKSNGYTPNDELTHIFTDWRKTHNQGVFDAYTAEMRAARKNKIITG
                     LPDAYGRGRIIGDYRRVALYGIDYLMEQKKKDHDMTGYHTMSEEVIRLREEITEQYRA
                     LAQMKEMAAKYGYDISKPAANAKEAVQWLYFGYLAAIKSQNGAAMSIGRISAFLDIYI
                     QRDLDNGVITEKEAQELIDHLVMKLRMVKFARTPEYNQLFSGFPIWATLSIAGMGLDG
                     RSLVTKNDFRLLHTLTNMGPSPEPNLTVLYSEHLPEGFRTYASRIAIESSSIQFENDN
                     QLREQWGSDDCAIACCVSGTVVGKDMQFFGARANLAKTLLYAINGGVDEVTGAQVGPE
                     YRPITSEILDFEEVKHNFMNMMDWLAELYVNTLNIIHYMHDKYSYEAAQLALMDSDLQ
                     RTFATGIAGISHAADSLSAIKYAKVKAIRNEKGIAVDFEVDGDFPKYGNDDDRADELV
                     SWILEYFMGQIKRHKTYRNSKPTTSLLTITSNVVYGKNTGNTPDGREAGKPLAPGANP
                     SYNAEQSGLLASLNSTAKMNYGCALDGISNTQTINPSGLGKEQDTRIENLRNVLDGYF
                     SKGAYHLNVNVFSNDLLRDAMENPMKYPNLTIRVSGYAVKFRDLTREQQLDVLMRTSH
                     DRM"
     gene            complement(10770..13220)
                     /gene="parC"
                     /locus_tag="ACG842_000012"
     CDS             complement(10770..13220)
                     /gene="parC"
                     /locus_tag="ACG842_000012"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HFQ0246585.1"
                     /translation="MENRQEVQELTLEEVMGDRFGRYSKYIIQERALPDIRDGLKPVQ
                     RRILFAMNKDGNTYDKGFRKSAKSVGNIMGNYHPHGDISIYEAMVRMSQDWKLREVLI
                     EMHGNNGSMDGDPPAAMRYTEARLSKLSGEMLADIEKETVDLVWNFDDTEKEPTVLPA
                     RYPNLLVNGSTGISAGYATEIPTHNLAEVIDGTIYMIDHPQASLEKLMEYIPGPDFPT
                     GGILQGKDEIKKAYETGRGKVILRSKTKIEAMKGNKQQIVITEIPYEVNKATLVKKMD
                     EIRLNKKIDGIAEVRDESDRTGLQIVVELKKDANAQGILNYLFKNTELQINYNFNMVA
                     IDHMTPHQVGLKDILRSYIEHRKQVITKRSQFDLAKAQKRQHIVEGLMKALSILDEVI
                     ATIRESKDKKDAKKNLVDVFQFTEEQAEAIVTLQLYRLTNTDITELQKESESLIAQIT
                     ELNKILSNDKELFSVMKKELREVKKNYSSARLTTIEDEIEEIKIDTQVLVAQEDVIVS
                     VTREGYVKRTSLRSYSASKPEEIGMREGDYLLYSGELSTLDHVLLITNKANVIYRPVH
                     ELPDLKWKDAGEHISQTISNLSVDESILAVFPYQKIQAEKTFVFISKNGLIKQTRMTE
                     FEPWRTYKSRPLSCMKLKAEEDELIAVYLKEDQPGLDVFLVTHQGMGLRYPLTDVPVV
                     GAKAAGVKSMNLKDGDRIINGLLVLAEGDTPIVIVTQRGAVKRMLAQEISQTSRAKRG
                     VTVMRELKKNPHRIIYMSEGRSKEITLMNQKGQQLTIDPTDFPIGDRTSNGSFAMDEK
                     KGGEVIEVIDAPEIFIES"
     gene            complement(13232..15277)
                     /gene="parE"
                     /locus_tag="ACG842_000013"
     CDS             complement(13232..15277)
                     /gene="parE"
                     /locus_tag="ACG842_000013"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HFQ0246586.1"
                     /translation="MAKKVNNEYNDSSIQVLEGLEAVRKRPGMYIGSTDSRGLHHLVY
                     EIVDNAVDEALSGYGSEIDVTIHEDNSITVADSGRGMPVGMHASGIPTVEVIFTVLHA
                     GGKFGQGGYKTSGGLHGVGASVVNALSKWLIVTIVRDGVEYQQKFKNGGKPDGTLKKI
                     GKTKKANGTTVHFLPDDTIFSTTKFSYEILAERLRESAFLLKGVKISLSDLRGEEPVK
                     EIFHYEEGIKEFVDYLNEEKDTLTPVVYFSGEKEGIEVEVAYQYNDGYSENVLSFVNN
                     VRTKDGGTHEAGMKAAMTKSYNEYARKVGLLKERDKNLEGSDFREGLAAVLSIRVPEN
                     LLQFEGQTKEKLGTPVARTVVDNVISEQMGFYLQENSEMSQMLVRKAIKAREAREAAR
                     KAREESRNGKKRKKGESLLSGKLTPAQSRNPKKNELYLVEGDSAGGSAKQGRDRKFQA
                     ILPLRGKVINTEKAKMQDILKNEEINTMIYTIGAGVGPEFSIEDCNYDKVIIMTDADT
                     DGAHIQVLLLTFFYRYMKPLIEAGKVYIALPPLYKVSKGQGKKQVIEYAWTDDELAAM
                     IKKVGKGYMLQRYKGLGEMNAEQLWETTMDPTSRTLIRVRIDDAAQAERRVTTLMGDK
                     VEPRRKWIENHVQFTLEEDGSILDKKEDTEISPSVSNDLLDEERADKNENNQLFEVE"
     gene            complement(15443..15877)
                     /locus_tag="ACG842_000014"
     CDS             complement(15443..15877)
                     /locus_tag="ACG842_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HFQ0246587.1"
                     /translation="MSVKNPTQETIFNYLEEAKNIAVVGLSNKPERTSYMVAQVLQDN
                     GYKIIPVNPLLAGEEILGEKVYANLTEIDDPIDIVDVFRRSEFLPEIAGDFLKTDAKV
                     FWAQLGIENKEAAEMLRKAGRTDIVMDRCIKIELGKLKENQK"
     gene            16004..16642
                     /gene="plsY"
                     /locus_tag="ACG842_000015"
     CDS             16004..16642
                     /gene="plsY"
                     /locus_tag="ACG842_000015"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HFQ0246588.1"
                     /translation="MKLILLLILAYLLGSIPSGVWIGKLFFKKDIRQHGSGNTGTTNT
                     FRVLGKTAGIVVLLMDILKGTLATCLPMIFHLSGINPLWFGVCAILGHTFPIFAGFKG
                     GKAVATSAGMLLGFNPVFFVYSCIIFIVSLFCTSMVSLSSMISAVLITLSTIVLPYVA
                     PVILAKPNWLLTIIAFLVSSFIFYRHKDNIKRIRNHTESRIPFGLNSSKKKS"
     gene            complement(16774..17649)
                     /locus_tag="ACG842_000016"
     CDS             complement(16774..17649)
                     /locus_tag="ACG842_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HFQ0246589.1"
                     /translation="MTVIIQNDQLIAEIAEHGAELISLKSKEHGLEYIWQGDPAYWGR
                     HAPVLFPFVGRLKDDQYVYQGKTYQMGQHGFARDMDFEVIEHGQEMASFILRSTPETL
                     EKYPFDFELVISYELGGDGITVHYQVANTGEKEMFFSIGGHPAFNVPLEEGLSFEDFY
                     LAFSPLKSRLRLPLKGPFIDMDQKTLGQTNTNLALNRELFEQDALVFETRGLNAYAIR
                     SEKSSHSVTLSYKDMPYVGIWSPYPKEAPFVCIEPWCGIADTVNSSGNLMEKVGINQL
                     GAHEIFKTKYSITVK"
     gene            complement(17731..18522)
                     /gene="codY"
                     /locus_tag="ACG842_000017"
     CDS             complement(17731..18522)
                     /gene="codY"
                     /locus_tag="ACG842_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HFQ0246590.1"
                     /translation="MTDLLTKTRKINTLLQQKNTFDMQADLPYDKMAVTLGGILDSNT
                     YIIGSDGVLLGYNERHDVNNERIKTMFVEKQFPESYTDTVAQLMKTEANIPITSDITA
                     FPVESREKYPFGLTTVVPIFGAGERLGTIILSRMEQSFADDDLVLAEYGATVVGMQIL
                     YQKSRSIEADVRSATAVQMAINTLSYSELKAVQAIFEALDGDEGRLTASNIADSIGIT
                     RSVIVNALRKLESAGIIESRSLGMKGTYLKVLNSRFKDELAKHSY"
     gene            complement(18540..19940)
                     /gene="hslU"
                     /locus_tag="ACG842_000018"
     CDS             complement(18540..19940)
                     /gene="hslU"
                     /locus_tag="ACG842_000018"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002409948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HFQ0246591.1"
                     /translation="MYELKQTPRQIVSELDEYIVGQHAAKKSVAVALRNRYRRLQLDE
                     KMQQDITPKNMLMIGPTGVGKTEIARRLAKIVNAPFVKVEATKFTEVGYVGRDVESMV
                     RDLVENAIQIVEKEQYSRVYTSAKKNAEKRLVKILVPGIKKEQKQSTNQFEQMMNLFN
                     ASQQNTAEPQEEVTEDIRINRRTVLEQLQKGLLDSREVTIEVEDQKKPAPMMNNGLEQ
                     MGIDLNETLGALTPKKKVERTVTVKEALELLIKEESAKLVKDADIHSEAIKLAEESGI
                     IFIDEIDKITSKSQQSGEVSREGVQRDILPIVEGSQVNTKYGIIQTDHVLFIASGAFH
                     LSKPSDLIPELQGRFPIRVELDDLTAEDFVKILTEPNNALVKQYVALIGTENVSVTFT
                     KEAIERIAQIAFDVNRDTDNIGARRLHTILEKLLEDLLFEAPDMQMGEITITEAYVDD
                     KLGSIAANEDLSRYIL"
     gene            complement(19954..20502)
                     /gene="hslV"
                     /locus_tag="ACG842_000019"
     CDS             complement(19954..20502)
                     /gene="hslV"
                     /locus_tag="ACG842_000019"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HslVU peptidase proteolytic subunit"
                     /protein_id="HFQ0246592.1"
                     /translation="MVESPFHSTTICAVEKDGKFAMAGDGQVTMGESVVMKGTAKKVR
                     RIYNGEVVVGFAGSVADAFTLEEKFEGKLNEYNGNLQRAAVELAQEWRTQQSMQKLEA
                     MLIVMNDKEMLLVSGTGEVITPDDGILAIGSGGNFALAAARAMKNYAQNDMSAKEIAE
                     NALNIAADICIFTNHNIIVEEL"
     gene            complement(20539..21444)
                     /gene="xerC"
                     /locus_tag="ACG842_000020"
     CDS             complement(20539..21444)
                     /gene="xerC"
                     /locus_tag="ACG842_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HFQ0246593.1"
                     /translation="MTEATWEQKFLRYLIVERGYSEKTKEAYEEDILHFKHFLEESGD
                     ADLLKAEYFTVRVYLSALADHRPSYSRNSISRKIASLRSFYQYLLKEEVIKENPFSYV
                     HLKKKNLRLPRFFYENEMQALFDSVAGDSPLDLRNRALLEVLYGSGIRLSECSSLSVS
                     DIDFDSEVMLIHGKGNKERYAPLGSFAQDALQEYFREGRQVLMTKYHEEHDIVFVNHH
                     GKPITPTGIEYVLNQMIKKSSLDSDIHPHMLRHTFATHLLNNGADLRTVQELLGHANL
                     STTQIYAHVTKESLQKNYRSFHPRA"
     gene            complement(21642..23018)
                     /gene="trmFO"
                     /locus_tag="ACG842_000021"
     CDS             complement(21642..23018)
                     /gene="trmFO"
                     /locus_tag="ACG842_000021"
                     /EC_number="2.1.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0047151 - tRNA
                     (uracil(54)-C5)-methyltransferase activity,
                     5,10-methylenetetrahydrofolate-dependent [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FADH(2)-oxidizing
                     methylenetetrahydrofolate--tRNA-(uracil(54)-C(5))-
                     methyltransferase TrmFO"
                     /protein_id="HFQ0246594.1"
                     /translation="MEITKKTSKNNRKEVLSLKMVNVVGAGLAGSEAAWQIAQAGVPV
                     RLYEMRPVKSTEAHHTSNFAELVCSNSLRGNSLANAVGLLKEEMRRLNSVVITSADET
                     AVPAGGALAVDRDSFSETITRKVKEHPLVTVINEEITEIPEGITVVATGPLTSHPLAE
                     SIKAFNGSDGFYFYDAAAPIVDKATIDMDKVYLKSRYDKGEAAYLNCPMTEEEFKAFH
                     EALINAEVAELKSFEKEKYFEGCMPIEVMAQRGFKTMLFGPMKPVGLEDPKTGKRPYA
                     VIQLRQDNAAASLYNIVGFQTHLKWGEQKRVFRMIPGLENAEFVRYGVMHRNSFMNSP
                     ELLKPTYQSTKREDLFFAGQMTGVEGYVESAASGLLAGINAARLAKEMEPVEFPQETA
                     IGSMAYYITHAEGKHFQPMNANYGLFPELPERIRDKKARYEAIAQRALKANEAIKTEL
                     EKTAIPNE"
     gene            complement(22987..25065)
                     /gene="topA"
                     /locus_tag="ACG842_000022"
     CDS             complement(22987..25065)
                     /gene="topA"
                     /locus_tag="ACG842_000022"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HFQ0246595.1"
                     /translation="MAYKYLVIVESPAKAKTIEKYLGRNYKVMASVGHIRDLPKSKMG
                     IDFENNYEPHYISIRGKGDVIKSLKAAAKKAQKVYLASDPDREGEAIAWHLAYLLGLD
                     LKEKNRVVFNEITKDAVKAAFKEPRTIDVDLVDAQQARRTLDRIVGYSISPILWRKVK
                     KGLSAGRVQSVALKIIIDREKEIREFVPEEYWSIDGNFKKARKKFKANFWGIDGKKKK
                     LPDAQSVKEVTARIDGTDFEVKKVEKKERKRNPANPFTTSSLQQEAARKLNFRTRKTM
                     MVAQQLYEGITLGKQGTVGLITYMRTDSTRIADTAKAEAAAFIEDTYGKEFSRHDHRK
                     IKNAQGAQDAHEAVRPTSVLRTPDELKQYLDKDQLKLYTLIWSRFVASQMTPAILDTM
                     KVTLEQNGVIFIANGSKIKFKGFMQVYVEGRDDGKEEKENILPELEEGDVVQSVDIEP
                     KQHFTQPPARFSEATLIRALEENGVGRPSTYAPTLDTIQRRYYVKLTQKRFEPTELGE
                     IVNSLICEFFPQIVDIHFTAEMEGDLDKIEEGTEAWVKVVDRFYKPFEKELTNAEEKI
                     EKIQIKDEPAGFDCDVCGHPMVIKLGKYGKFYACSNFPDCRNTKPIVKEIGVTCPVCH
                     EGQVIERKSKKNRLFYGCSRFPECEFTSWDKPVGRNCPKCDGYLVEKKVKGGKQVVCV
                     NGDYEEDIQK"
     gene            complement(25187..26044)
                     /gene="dprA"
                     /locus_tag="ACG842_000023"
     CDS             complement(25187..26044)
                     /gene="dprA"
                     /locus_tag="ACG842_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HFQ0246596.1"
                     /translation="MYQIEENLLKLAYTKGISSQGKWALANWMMQYPYKEIGFEDIIK
                     IGKITKHRELFRKSWQEIHANWGKIQSKQSFITCFDPKYPPQLLHLTYPPIVLFYNGD
                     LSLLSCNMLSVVGGRHTSALAKKTVYYLLKPVIEAGYVIVSGCAKGIDTYGHQAAISH
                     GGRTIAVIGTGIEQVYPAENRDLQEVIANDHLLLSEYPPQSGPKKHCFPMRNRIIAAL
                     SKGTCVFEAKKKSGSLITAQQALDLGRTVFSVPGNILTEHSTGCHHLIQDGAVCTVSG
                     EDILAEFKE"
     gene            complement(26100..26867)
                     /locus_tag="ACG842_000024"
     CDS             complement(26100..26867)
                     /locus_tag="ACG842_000024"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HFQ0246597.1"
                     /translation="MTESIQSIKERLKSIQRIDDPTLESLRNDPRKGVQQALASFERR
                     LKKEALIKEKYEEMFCFEKQGYAQGHQWIAGIDEVGRGPLAGPVVAAAVILPKNEIIL
                     GLNDSKQLSEKKRELLYQQVQEKALAIGIGIVDQQTIDDVNIYEASKLAMIQAVEKLS
                     IVPSYLLIDAMTLDLPIQQEKIIKGDARSVSIAAASIIAKVYRDQLMKEYDVLYPGYG
                     FSHNAGYGTKEHLEGLERQGITPIHRKSFAPVKKYLQ"
     gene            complement(26860..27720)
                     /gene="ylqF"
                     /locus_tag="ACG842_000025"
     CDS             complement(26860..27720)
                     /gene="ylqF"
                     /locus_tag="ACG842_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HFQ0246598.1"
                     /translation="MTIQWFPGHMAKARREVSEKMKYVDIVFELVDARLPLSSRNPML
                     DQIIQQKPRLVLLNKADLADPQQTKLWQEYFRKQGHLALAITAQESKGIKGLVPAAKE
                     ALKEKRARDAARGIKPRAIRAMVLGIPNVGKSTLMNRLVGKKIAQTGNKPGVTKGQQW
                     LRLGNELELLDTPGILWPKFEDEEIGKKLALTGAIKDQLVHLDDLAIYGLDFFARYYS
                     GRISDRYGLSIEQEEKLAPELLMDITAKRGYKEDYDRGSELVVHDIRQGKLGRYTLDR
                     CEEMEQKSDD"
     gene            27980..28378
                     /locus_tag="ACG842_000026"
     CDS             27980..28378
                     /locus_tag="ACG842_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFQ0246599.1"
                     /translation="MNSMVFVNFPVADVNRSVAFYEKLGFKKNEEFSTEEAGSMVWDD
                     TFWIMLLSHDFYKKFIKDKEIADNKKTSGALISFSVENADEVKKFAQIAKENGGTFHE
                     VDMGMPEEEMFSLEVQDLDGNTLEPVWMKM"
     gene            28615..29157
                     /locus_tag="ACG842_000027"
     CDS             28615..29157
                     /locus_tag="ACG842_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HFQ0246600.1"
                     /translation="MNTLIAKDVFRLPEMNARLLSTSALLLALDVILYKIAIGPAYFQ
                     ITFGFLSMALMGYLYGPIWAGLLEMLSNTLNFTIFGSGTFQAAFLITAFLGGFINGLF
                     LYHKKINLPSVIAAQLLIMIPVSLFINSWLMSVLFGADFKVIFLGRFIRNMIQIPIQI
                     MVVYSFIKTLEARKVFKRAV"
     gene            complement(29240..29791)
                     /gene="lepB"
                     /locus_tag="ACG842_000028"
     CDS             complement(29240..29791)
                     /gene="lepB"
                     /locus_tag="ACG842_000028"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HFQ0246601.1"
                     /translation="MTKKQRYINRFWLVFKYLLVSVFLAFMLRGFLFIPVPVEGNSME
                     NVLKQGDMVVMEKFSEIRRFDIVVFQLADGTIYIKRVIGLPGENVSYQNDQLKINGKV
                     VKEPYLTKNLKSDHANASYTTDFTLQELTGQSKLPEDNYFVLGDNRRVSKDSRSFGTI
                     NKTDILGKARFVYYPLDEIKWIQ"
     gene            complement(29943..30170)
                     /locus_tag="ACG842_000029"
     CDS             complement(29943..30170)
                     /locus_tag="ACG842_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HFQ0246602.1"
                     /translation="MNRSFFHYLMTLRGGGTHDALSVFATEAGKDIQFPKHSSSYEEI
                     SNYLELNVDYLPSMDIFDEAWEKYLENNHDI"
     gene            complement(30167..30685)
                     /gene="msrA"
                     /locus_tag="ACG842_000030"
     CDS             complement(30167..30685)
                     /gene="msrA"
                     /locus_tag="ACG842_000030"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HFQ0246603.1"
                     /translation="MERAIFAGGCFWCMIQPFDTLPGIHTVMSGYTGGHVPDPTYEQV
                     KSQTTGHTEAVEIIYDPTLISYEQLVELYWQQTDPTDAFGQFEDRGDNYRPVIFYLTE
                     EQRIIAEKSKKQLAESGRFEQPIVTKIEPAQPFYLAEEDHQEYYKKHPENFKRNHARR
                     AAFIEENWRNEG"
     gene            complement(30705..31418)
                     /locus_tag="ACG842_000031"
     CDS             complement(30705..31418)
                     /locus_tag="ACG842_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpmS family protein"
                     /protein_id="HFQ0246604.1"
                     /translation="MKTRSDVSSNKTKKTNYWKFAFLILLGVVLGSTVFLGSRIFANR
                     EPELPEIPALTERQGDPVLTINSNKEKVNQIISFFLSEYQKDSDIEYKFYLENEALLN
                     GTFEVLGFPIDFYLYFDPYVMENGNVQLKARSLSIGTLSLPIKDVMNMIKRNYKLPDW
                     IEVNTDDLTIMLRLDQFRMQNGMYIKADKIDLVNDDIRFSLYLPKDTSDASNNSNTNK
                     TSTSSTESKTKENKKSSDE"
     gene            complement(31421..32317)
                     /locus_tag="ACG842_000032"
     CDS             complement(31421..32317)
                     /locus_tag="ACG842_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HFQ0246605.1"
                     /translation="MKLTKRHLILVLFPLLIAIIIYAGLAASIPKAGPLMKPVTTVRE
                     DPNKKEKLHYIAIGDSLTEGVGDQTKQGGFVPLVANDLKDRYNLTAVETENYGVNGER
                     SDQILKRVKTKEEIRNNIKTADFITLTVGGNDLMKVIQNNLFGLTVNSFKKPLKNYQE
                     NVTELLEEFRSLNKTAPIYILGIYNPFYLNFPEITDMQTIVNNWNKGTETVTKKMPNC
                     YFIPINDLLYQGLNQEVGVTEEGTDQTTSTTENLNGIKNNVLYEEDHFHPNNLGYQLM
                     ANAVRDQLIETQNQWLEKEDDQ"
     gene            complement(32475..33317)
                     /locus_tag="ACG842_000033"
     CDS             complement(32475..33317)
                     /locus_tag="ACG842_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HFQ0246606.1"
                     /translation="MTNVKIVTDSSCTMEKNVRDALGIRVMPLSIMIDGTVYPDDDQL
                     DGERFMEMMAKAKNLPKTSQPPIGEFISMYDELGEDGSEVISIHMTKGLSGTVEAARQ
                     ASNLTKTKVTVIDSDTTDQGLSFQVIRAAELAKEGKTVEKILEEVKKVQDNTKLYIGI
                     STLDNLVKGGRISRTTGLLSSVLNIRVVMNFAHSELIPVTKGRGKKTFDKWFNNLKNE
                     LKELPNVRQIGISHADAKELAQEFKEGLQELFPDMHIPVLHTNPVIATHTGKDAFAIM
                     YYVD"
     gene            33449..34102
                     /locus_tag="ACG842_000034"
     CDS             33449..34102
                     /locus_tag="ACG842_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HFQ0246607.1"
                     /translation="MEKTKFNRTYEILNEVFNAVTHGIGTGLSIAGLVVLLVKGAHLH
                     SSLHIVSYAIYGSMMILLFLTSTLFHSLIFTKAKKVFQVFDHSSIFLLIAGSYTPFCL
                     LSIRGWLGWTLFVLIWLFAILGIIYKALTLHKRERVSKVSTIIYIFMGWLCVVAAVPL
                     YHSLGMTGVILMVAGGLSYTIGAFFYSLNSIRYMHVVWHLFVMLGAGFMYFSVLFFT"
     gene            complement(34165..34692)
                     /locus_tag="ACG842_000035"
     CDS             complement(34165..34692)
                     /locus_tag="ACG842_000035"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HFQ0246608.1"
                     /translation="MLAAIWAQDENGLIGKEDRLPWRLPNDLKFFKQMTEANTLVMGR
                     KTFEGMGSRPLPNRQTIVLTRDSSYKAEGVHVMHSVEDVLALEKETDGIFFIAGGSAV
                     YQEFLPFCTILYRTVIHHVFDGDAYFPPVDWSDWSLINTSQGEMDEKNRYPHQFETYQ
                     RNENPVKSGTGELTI"
     gene            complement(34712..35659)
                     /locus_tag="ACG842_000036"
     CDS             complement(34712..35659)
                     /locus_tag="ACG842_000036"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HFQ0246609.1"
                     /translation="MEQAYLDLGRKLLEEGHRKEDRTGTGTLSLFGYQMRFDLQKGFP
                     LLTTKRVPFGLIKSELLWFLKGDTNIRYLLQNKNHIWDEWAFERYIKSPDYNGPDMTD
                     FGHRCLTDPEFNEEYKKQSKLFCEKILTDDSFAETYGDLGHIYGYQWRHWETKDGGFI
                     DQIKQVIEAIKKTPDSRRLIVSAWNPEDVPSMALPPCHTMFQFYVQEGRLSCQLYQRS
                     GDVFLGVPFNIASYALLTHLIAHETGLAVGEFVHTLGDAHLYINHIEQMKEQLSREIR
                     AFPTLVLNQEKQSVFDFEMEDIQLEGYDPHPAIKAPIAV"
     gene            complement(35671..37554)
                     /locus_tag="ACG842_000037"
     CDS             complement(35671..37554)
                     /locus_tag="ACG842_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HFQ0246610.1"
                     /translation="MKELKVTDLKKTYGEKDLFDQISFLVHEKDRIGLIGTNGTGKTS
                     LLNIIAGIDSSDGDRQTVFYPTDYRIGYLSQAAEFSDELTVLQAVFQGNSPLIQTVRA
                     YEEALIELGENGEDPDVQKRYAKAEEQMNKEDAWTTDTNAKIILQKLGISELDKKIST
                     LSGGQKKRVSLAQVLIDEPDLLLLDEPTNHLDYEAIEWLENYLKQYRGALLMVTHDRY
                     FLDRVANRIFELSFGKLYEYKGNYEAYVLEKAERDRVEVEQEEKRKRLYKQELAWMRA
                     GVQARGTKQQARINRFEDLKENLYQVNQEDDLELNLATQRLGKKVLEIKDGSYRINDQ
                     TLLEHLDLLIQSRERLGITGKNGAGKSTLLNILAGRIPLDSGTMSIGETVHLAYYTQE
                     NEEMAPDKRMIAYLQEAAEEAKTADGSQIGVAELLERFLFPRFMHGTLIGKLSGGEKR
                     RLFLLKLLIQQPNVLLLDEPTNDLDIATLTILEDYFRSFPGAVITVSHDRYFLDKVAD
                     KLLVFQGNGKQELYYGNMSSYLLKQKETQQPAEKAKPKIQSKEPAEKKKLSYMEQKEW
                     ETIEDEIAELEEKISLLQEEMNHQGDNFTRLQELQNDVSETEAQLEEKMARWEYLSEW
                     VED"
     gene            complement(37717..39795)
                     /locus_tag="ACG842_000038"
     CDS             complement(37717..39795)
                     /locus_tag="ACG842_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HFQ0246611.1"
                     /translation="MEFAELVIVFAVTITLSNIASRILPMIPAPLIQIFLGVVLGLTE
                     WGQSIDFEPELFLVMIIAPLLFREGEKADISSILKNFGTILFLAFGGVILTLVGVGAT
                     LSFLLPSVPLAACFAFGAALGPTDAVAVSSLSGRVNIPKKAMHILEGEGLLNDASGVT
                     AFQFALGALITGSFSAVNAGMSLVVSSIGGALIGFLLVWFKQKIIHLIEKASAQDVTG
                     YLLIELLLPFLAYVLAEAFDVSGIIAAVAAGILQASGFRKISVFDAELSSLSHSTWTT
                     IAFTLNALVFIFLGIELTQVFSPVWGDGLYPNGLLLAIIVLISVMLFVIRFISISLFY
                     VFKDGSKKFKKQLNEILILTFGGVKGTVSLATIFILPFSINNMMFYQRSLLLFLTAGV
                     ILVTLVIGIIVLPMLTETEEAKSTDLNALMILEEVVEVLRKEIKEIDRNTKEFLATEA
                     VIENYQERIRDLYLEDLTDDEKQEVQEIQALILSIERDGLDESYRSGKLSSNGYRFYS
                     RFLSRFEHSITSQILSFIGFWFIVVRRLMRIVLHPKMFWQRRYADRQTFISDEDIQEI
                     RETYQKNTQLIIESLDNLTDIYDETMVQFFIQQRKLEGIKMNSGNLISSWMIQQDSLF
                     TKKMLRGYYLERKIIDEYEVAEKITTFSANSYRRNINLLESYTMNKPSDNFSFRFAFR
                     AKSKKTSPIK"
     gene            complement(39808..40083)
                     /locus_tag="ACG842_000039"
     CDS             complement(39808..40083)
                     /locus_tag="ACG842_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0246612.1"
                     /translation="MRESFEQQKKLLHDRYGALSMDDRRQILCKLRKRNILMYRQLER
                     LKHDLLRLESKRVQCELEGNQTQVEVVETKILKKKEQFLKMLTQNKK"
     gene            complement(40445..40900)
                     /locus_tag="ACG842_000040"
     CDS             complement(40445..40900)
                     /locus_tag="ACG842_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0246613.1"
                     /translation="MSKEMTALKFYFRNGETWTINRRHIGDLWIKQITTSFGRINGSE
                     FVEIHPCAGFKIEIFHEGDAVATHDINLGGLEMGMFNRALKYEDIERMEILYRNGTPD
                     LVYFPYLDKGTEGLDNQYQSTKISEKTGNLYIVINPDQRVEDVYGEFFE"
     gene            complement(41054..42262)
                     /locus_tag="ACG842_000041"
     CDS             complement(41054..42262)
                     /locus_tag="ACG842_000041"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001680 - tRNA 3'-terminal CCA addition
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HFQ0246614.1"
                     /translation="MKLKQLPLEYQKAIPVLKKLENAGYEAYFVGGSVRDILLNQPIH
                     DVDIATSAFPEEIKQLFPKTIDIGIEHGTVLVLENDEQYEITTFRTESTYQDYRRPDH
                     VEFVRSLAEDLKRRDFTINAFALKEDGEIIDLFDGLQDLEEQILRAVGNPHERFHEDA
                     LRMMRGLRFVSQLGFKLEEETFYAIKENHELLGKISVERINVEFIKMLLGNERAAGIH
                     AFIETECYICCPGLKEEGEALLRFAELPSVPLKEESQAWALLISQLDLKENEIRSFLK
                     SWKCSNQMIKDVQALVQGLRQRETGALEPFELYFLGKKHALQVEELMIYFDETPQMDA
                     VGESYDRLPIKSLSELAVDGKILLEETGKKPGKWVSEALQLAEKAVVEQRIKNDKKQV
                     LDFLSKEKLI"
     gene            complement(42374..42706)
                     /locus_tag="ACG842_000042"
     CDS             complement(42374..42706)
                     /locus_tag="ACG842_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HFQ0246615.1"
                     /translation="MDKKRSLYSMQQEVDEYIQQFKVGYFSPLAQMARLTEEVGELAR
                     EVNHTYGEKSKKASEPVNSVAEELGDVLFVTMIMANSLNIDLTEVFEKNMEKFNQRDH
                     NRFERKDE"
     gene            42854..43729
                     /locus_tag="ACG842_000043"
     CDS             42854..43729
                     /locus_tag="ACG842_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFQ0246616.1"
                     /translation="MVKRKTVKEVLFIIIGTSIYAFGLVYLNIANHLAEGGVSGITLI
                     LRALFGIDPAYTTLLINIPLILLGGKILGKRSLAYTVLGTLSLSFFLWIWQRVPLEIN
                     LQHDLLIVSLLAGLVAGVGSGIVYRMGGTTGGSDIIARILEQNYGITMGRSLLAFDII
                     VLLASLTYIDLKRMMYTLIVSYVFSRVIDSILDGGYSAKGLLVVSNKSEEIAPLLMTG
                     LQRGVTFLSGEGAFSGNSKKIIYMVVSSSELTEAKRIVHEIDEKAFLSIINVHEVEGQ
                     GFTYLKPQTRLLKKL"
     gene            complement(43811..44392)
                     /locus_tag="ACG842_000044"
     CDS             complement(43811..44392)
                     /locus_tag="ACG842_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ReoY family proteolytic degradation factor"
                     /protein_id="HFQ0246617.1"
                     /translation="MLIDVREKKNFLTWMVNHVSFSRREVFWILNYLANHEAILSNVH
                     FVEGANFADRGLQISDLSVEGEPMKLFLQGKEFTDTDQIFHEIRLNWKKPLYVECIFE
                     NAWQTREYLSILEDNPFSSWDSSVSEEDTELIERYFQEKEEEAKLKMLYAQIDQALED
                     GDRDAFLKLSDEVNRKILLQTQKKSNRVSEHNN"
     gene            complement(44398..45657)
                     /locus_tag="ACG842_000045"
     CDS             complement(44398..45657)
                     /locus_tag="ACG842_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFQ0246618.1"
                     /translation="MNTNSEKMLQALSDEDLSQAQIFLEKALKEDPADILAELGEELL
                     AIGFLIEAKQIFEQLINQYPENDGLNIPLAEIAIEDNEIDLAFEYLEKVDKQSDYYPQ
                     SLLAIADLYQVIGIPEVSEAKLKEAAGLLPDEPLIQFALAELYFSVDRFKEAAVVYET
                     LLASGINEISGISMQERLGQSLSMQGEFEAAIVPLEEALKEERTDDRLFQLAFTNLQL
                     KENQQAINYLEELREVNPHYQSLYLYLGQALQEEEMIEEAQTVLEEGIKENPYQVELY
                     HLASENAFRLHDRQRAEELLLKALEVGDKQDETLLTLSNLYLDDERYEDVIQVINQME
                     ETANPYAEWNLAHAYNELEDFTVAAVHYEQAYHELSHEPDFLKEYAFFLREEGQLKRA
                     QELLTYYLTLEPGDMEALSLLDDLTER"
     gene            complement(45659..46705)
                     /locus_tag="ACG842_000046"
     CDS             complement(45659..46705)
                     /locus_tag="ACG842_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0246619.1"
                     /translation="MELDYQVEDALTKVFYGETLVSQLRELSIDQPILFLTNQRYYDL
                     FADKINPLFANQANNDWYICANTQCNHLTELKNLLDFVKRYPENQSMFIIGFGNEGIM
                     DLAGFFQKHTTLDSSLWMIPVSIRSLSRALTPQRTILQLPNRVVLQTINLPERIVYDQ
                     TISERQVDGKQIDFLTFICCGMICDHRFLQNLYKNYPNKKQLTTRPFAGMIEEMIYFY
                     QLEAENLSNYGKLFEQAFYLTENGHLLSASMKKFLGMMFHFVWNLQAYPFDFQLKNFF
                     VWLDLLGYPIHWLEQISKMEYLEKVLFLAEKSKKILVLEKIGIIDGYQHPNEKELLRT
                     MTVFQQIVSEIRGN"
     gene            complement(46940..47215)
                     /locus_tag="ACG842_000047"
     CDS             complement(46940..47215)
                     /locus_tag="ACG842_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007208657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HFQ0246620.1"
                     /translation="MANKAELIEKVASATDLTKKDATAAVDAVFSTIQDALANGEKVQ
                     LIGFGNFEVRERAARKGRNPQTGEEIEIPASKVPAFKPGKALKDAVK"
     gene            complement(47458..48768)
                     /gene="der"
                     /locus_tag="ACG842_000048"
     CDS             complement(47458..48768)
                     /gene="der"
                     /locus_tag="ACG842_000048"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HFQ0246621.1"
                     /translation="MANPTIAIVGRPNVGKSTIFNRIAGERISIVEDTPGVTRDRIYA
                     KGEWLGREFSVIDTGGIDLGDEPFMDQIKHQAEIAIEEADVIICVVSGREGVTDADEM
                     VAKILYRSNKPVILAVNKVDNPEMRNDIYEFYSLGLGDPYPVSGSHGLGIGDILDEAV
                     KYFSEETEEEDDDTIKFSLIGRPNVGKSSLINAILGEERVIVSDIEGTTRDAIDTHFV
                     SESGQKFLMIDTAGMRKRGKVYENTEKYSVMRAMRAIDRSDIVLMVLNAEEGIREQDK
                     RVAGYAHEAGRGIIIVVNKWDLVKKETNTMRDFEQEIRDEFRYLDYAPIVFVSAVTKQ
                     RLERLPEMIEQVSMNQNLRISSAVLNDIIMDAVAINPTPTDKGKRLKIFYATQVAVKP
                     PTFVVFVNEEELMHFSYERFLENQIRKAFTFEGTSIRIIPRRRK"
     gene            complement(48950..50179)
                     /gene="rpsA"
                     /locus_tag="ACG842_000049"
     CDS             complement(48950..50179)
                     /gene="rpsA"
                     /locus_tag="ACG842_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HFQ0246622.1"
                     /translation="MTEFDQNQENNQSMEDAMKSVQEVKVGDVVKGEVLVIEDKQVIV
                     GIEGAGVEGVVPAKELSTLPVEDISEVVKVGDVLDLVVISTIGKDKENGSYLLSKRRL
                     DAKKVWEDIERDFQEGKVIEAPVTNVVKGGLVVDVGVRGFVPASMVEDHFVADFSDYK
                     GKTLSFKIIEIEPSENRLILSHKAVVENEREEKKKEILSTIHEGDVLSGRVARLTDFG
                     AFVDLGGIDGLVHVSEISHSHVAKPSDVLAVNDDVNVKVLSINPEQERVSLSIKDTQP
                     GPWSEIDTKAPVGTVLDGTVKRLTSFGAFVEVFPGVEGLVHISQISHKHIATPHEVLH
                     EGDEVKVKVLEIHPEEHRIALSIKALEEKPASEEEPKNVESYELPEENTGFTMGDILG
                     DALKEEVEEKATDDSEK"
     gene            complement(50314..50994)
                     /gene="cmk"
                     /locus_tag="ACG842_000050"
     CDS             complement(50314..50994)
                     /gene="cmk"
                     /locus_tag="ACG842_000050"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - (d)CMP kinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HFQ0246623.1"
                     /translation="MTQISIAIDGPASSGKSTVAKILAKELNYIYTDTGAMYRAVTYL
                     AITNNVSFSDEEGLVSLIKKYPISFSQQEDGQHVFIASKDVTLEIRQPDVTKAVSEVS
                     AHGKVREELVAVQRKIGERGGVVMDGRDIGTAVLPNAEVKIFLVASVSERAERRFKEN
                     QEKGIPTDLETLTKEIQERDHYDSTRDVSPLKQAEDAVRIDTTGKSIPEVVAAIKAVV
                     LKKGYLLF"
     gene            complement(51043..51678)
                     /locus_tag="ACG842_000051"
     CDS             complement(51043..51678)
                     /locus_tag="ACG842_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAG1386/EF1546 family surface-associated
                     protein"
                     /protein_id="HFQ0246624.1"
                     /translation="MSRKDRHQSTENNETQEPWEQPIYDTDDEVSSRSSQRQQKKGNT
                     LFLSLFLILLVLCIAIPAGAYFWIRNGSSNNATAATSSETSTSVVESSTSTTKEESTE
                     TSTVESSTIAEESTVSQDTGVTSESSIAAETTPTSSSEATYQAGDSSSNAAETTPSSS
                     AAAGSTTTVQAGEGPKQVAERAGITTDQLFELNGLDPNNFMLYPGQELRIK"
     gene            complement(51744..53147)
                     /locus_tag="ACG842_000052"
     CDS             complement(51744..53147)
                     /locus_tag="ACG842_000052"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043138 - 3'-5' DNA helicase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HFQ0246625.1"
                     /translation="MLINELKKYFGFDSFRPGQEEIIQALLDGEDTLAILPTGTGKSL
                     CYQLTGYLMEGLVIIVSPLLSLMEDQVTQLQKRGEKRVAAFNSLLSQSERRYVLRHLS
                     QYKFLFLSPEMLTNPSLLEHLKKQNIALYVVDEAHCVSQWGVDFRPEYQQLGKIRKLL
                     GNPVTLALTATATDLVARDIRHVLFDRPPKEIRQSVNRQNISLFVRKTQQKEQELEHF
                     MERAQGAAIIYCATKKEVERLYHLFRERFTVGYYHGGLDAAQRRQLQQQFVKNQLQFL
                     IATNAFGMGIDKSDIRYVVHYDLPDSLENYVQEIGRAGRDQEESAAILFYQSGDERIH
                     YFFNQLSREQRQSFELYLEYATEQAPFDELQKKWMELIQQSEKPENWVERLKRQEKEK
                     EFRLQQMLRYINEENCRRKFILAYFGEKLSEKPQNCCDIDGAKAELLPKRKLFSKTEQ
                     LHWGTILLNLFKKNMEH"
     gene            complement(53134..54165)
                     /locus_tag="ACG842_000053"
     CDS             complement(53134..54165)
                     /locus_tag="ACG842_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0246626.1"
                     /translation="MIVFILSLFSSGHKLRISSLYQLLVGKRTTSVLIYGFTHELLFI
                     HNSFPELKQDKFYQILQKIAQQGWIEINENEAKLTPAGADMLSEHQIEHTGLRFDRYG
                     RTGETSWRLIKFAVQVISNLATGIQDYLPAETSPFYTFQLKKWLSESQLPREILIDTA
                     YESLVQLFSEIPEEAADFLANQLSGNERTGLLPYQLAKNNDESAVYLQQSRCIHLLLA
                     QIEKRPDSLWHALIDSLLQQNFNQSMMITKQMFMNGQTIDQIMAIRHLKRGTVTDHLI
                     EWALFFDDFPYEWILSAETIERLEPNKDSVREWRFSEWNVDGQLDYGEFRLYQIYLLR
                     KEAIQNVNK"
     gene            54196..54417
                     /locus_tag="ACG842_000054"
     CDS             54196..54417
                     /locus_tag="ACG842_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HFQ0246627.1"
                     /translation="MSLLCKLVPERCIACGLCQIEAPDIFDYDEEGIVLFAQEPEAHQ
                     QFVMPEEETYVKKAYQKCPVRAILLENKI"
     gene            complement(54582..55319)
                     /locus_tag="ACG842_000055"
     CDS             complement(54582..55319)
                     /locus_tag="ACG842_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HFQ0246628.1"
                     /translation="MKIFRRILIGLVIILGLLGGAGYYYIQQNTYEPSSEAQVLVKEG
                     KNKEDYLFFEGEKKNHGKSPLLIFYPGALVEPESYSIWAQDLAKNGFSVAIVKMPLDL
                     AVMGGNKADKVKEDFPNSDYVIGGHSLGGVMASRYVNKNHSDKHLKGVFFMASYPDKK
                     GTLTESAVPVLSITATKDKVLNQEAYQEAKQYLPERTEYVSINGGNHGGFGSYGEQKG
                     DGEAEISNADQQTVIVNTLENWLKKLK"
     gene            complement(55472..56812)
                     /locus_tag="ACG842_000056"
     CDS             complement(55472..56812)
                     /locus_tag="ACG842_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HFQ0246629.1"
                     /translation="MKKFVTEQHHEQALESLSELIRIPSVLDEADSGQGHPFGKKVIE
                     ALDKVLEISEKLGFRTFKDPEGYYGYSEIGSGDELFGILCHMDVVPAGDENNWETKPF
                     DPTIKDGWLVGRGSQDDKGPSIAAMYAVKALIDAGVEFKTRIRFIFGTDEENLWRCLE
                     KYNEKEEGITQGFAPDAEFPLIYAEKGLLQAYLTGPGTNEFSVKAGGALNVVPDAAPY
                     SGEKLSEVKEALKKHEFDFEEQGEGIVVLGKSIHAKDAAQGVNAISRLAIALSEVFDF
                     GPINFLGKLVQENATGEAVVGKTEDEQSGELTMNFASLEITPEQTKIGVDMRIPVTFK
                     KDDLVAKLTKTAEKYGLTYEEFDFLDSLYVPLDSELVKNLLGTYRDITGDMTEPFVSG
                     GATFARTMNQCVAFGAMFPDTPDFMHQANERWELSSMYKAMEIYAEAVYRLCAK"
     gene            complement(57049..58572)
                     /locus_tag="ACG842_000057"
     CDS             complement(57049..58572)
                     /locus_tag="ACG842_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HFQ0246630.1"
                     /translation="MEQTKKKKFQMPSAYTVLFIIIAVIAVLTWFIPAGHYSVDDAGN
                     IVAGSYERVQQNPQGLWDIFMAPINGMLGVPAGELTKETPAAIPISFFILVVGGFLGI
                     INKTGALDAGIASVVKKFKGKEKMLIPVLMLLFALGGSTYGMAEETIPFYALLIPVMM
                     AVGFDTVVAVAIVLVGSQVGCLASTVNPFATGVASQAVGISMGEGIGLRFLMFVILLA
                     ISIVYVYRYASKIEKDPTKSLVYNQRKEDMKHFDIEAIGRQEVITKKQKHVNVLFFIT
                     FGIMIISLIPWTTLNANFTIFESLTNWLTNLPVLGTVLGKSMLPLGDWYFPEITMLFL
                     VMSIVVAITYGMKESEFVSEFLAGASDLIGVAIVVAVARGIQVVMNNGLITDTILHWG
                     EQGLSSLSSSVFIIITFIFYIPMSFLIPSTSGLAAATMGIMGPMGEFAGVGKDLVITA
                     YQSASGLVNLITPTSAIVMGAVAIARFDVSVWWKFTGKLIAILFVAICVILGVAAMF"
     gene            complement(58950..59561)
                     /locus_tag="ACG842_000058"
     CDS             complement(58950..59561)
                     /locus_tag="ACG842_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFQ0246631.1"
                     /translation="MKNTRVQKMVAVAMFAAIALVLQYFAFPIMPAFSFLKIDFSDIS
                     VLISMFLFGPAAGVATAFLRSVLHLLTTGFSPDNLVGDTASFLSTTIFTFPIYYFFSV
                     KKQHKGRNKFLGVITGTIALTIFMSIANYFVITPLYLMFLGLNATQMLGMSLANYVLI
                     GIVPFNLIKGFIVSAVFLVLHAQLLPWLSRKQHQFEQRHTAVK"
     regulatory      complement(59684..59809)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(59891..60607)
                     /locus_tag="ACG842_000059"
     CDS             complement(59891..60607)
                     /locus_tag="ACG842_000059"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HFQ0246632.1"
                     /translation="MERLQKVIAHAGITSRRKAEEFILAGRVKVNGQVVRELGIKVGK
                     HDTVEVDEVPIYQEEYGYYLLYKPKGVISAVADDKGRKVVTDLLPMVKERIYPVGRLD
                     YDTSGILLLTNDGDFAQRLTHPKHEVDKVYVAKVKGIATKSMLAPLTKGIKIEGRKTS
                     PAKFQILSVDPKTNHSIVELTIHEGRNHQVKNMLQAVGLPVQKLKREKYGDLTLQGLR
                     PGDYRELNKKEVSSLLNHSK"
     gene            complement(60613..61182)
                     /gene="scpB"
                     /locus_tag="ACG842_000060"
     CDS             complement(60613..61182)
                     /gene="scpB"
                     /locus_tag="ACG842_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HFQ0246633.1"
                     /translation="MTKLSELEALLFIVGEEGISNEELSGLLNLSEAEVTSLVKELKE
                     SYTQNSHSALHIFETESHYVLTTKKELAPLLKRYAQGTSNTLSQAAVETLAIIAYKQP
                     ITRAEIEQIRGVQVSGAVQRLAAHKLIEEKGRVEGPGRPILYGTTGYFLDYFGLKSLE
                     ELPDTQEMEISLEEDVSPDLFFDGMNKGE"
     gene            complement(61166..61936)
                     /locus_tag="ACG842_000061"
     CDS             complement(61166..61936)
                     /locus_tag="ACG842_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HFQ0246634.1"
                     /translation="MNELTEINIKLDVFEGPLDLLLHLIQQMEIDIYDIPIATITEQY
                     MNYIHTMKTLELAVAGEYLVMAATLMAIKSKTLLPVQEFVTDDEELWEEDPREELVTQ
                     LLEYRKYKYAAERLTEKAEERSLYYTKEPMNIDGLADAEKPLKRGQVKKIDLIRAMQK
                     ILERKKFSQKVEKTITPDDTTIEERMVFIEERIKRNAHGCTFDSFFESPSKSEVVTTF
                     MALLELMKKGQIIAQQKENYESIMLYPAVGDTEHDEIK"
     gene            complement(61933..62304)
                     /locus_tag="ACG842_000062"
     CDS             complement(61933..62304)
                     /locus_tag="ACG842_000062"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0246635.1"
                     /translation="MLTHYRKENRKIAMGLLSFHKKLTGKTSLLKEIDTYESEDDYEL
                     FFFTPEGSTNIQGIIGIYWATNDQLIIHDISLNPSYRGENMGFQMLDELQEKYPDVSI
                     RATEITEPYIGKWLAKKKKKE"
     gene            complement(62323..63210)
                     /gene="xerD"
                     /locus_tag="ACG842_000063"
     CDS             complement(62323..63210)
                     /gene="xerD"
                     /locus_tag="ACG842_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HFQ0246636.1"
                     /translation="MKEQINDYLHYLNIERGLSANTRQSYERDLEQYLHFLQEQKIAS
                     WQDVDRFLVIRFLEKLHEEKKATATVTRMISSLRRFHQFLRQERITDHDPMQHIDTPK
                     KAQKLPSTLTLTEVEKLIEAPDTTKTLGIRDRAILEVMYATGMRVSELIGLKLGDLHL
                     SLGLVQTVGKGDKERIIPLGDYAIQWLERYLDEARPLLVKDASEMHVFVNNHGKGLSR
                     QGIWKNLKQLVREAGIYKEVTPHTLRHSFATHLLENGADLRTVQELLGHADISTTQIY
                     THITKKRMTDVYKQHFPRA"
     gene            complement(63306..63779)
                     /locus_tag="ACG842_000064"
     CDS             complement(63306..63779)
                     /locus_tag="ACG842_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HFQ0246637.1"
                     /translation="MVMNAISAMKKTKQQLHEAGFKLTPQREATLLVLLENEKEHLSA
                     EEIFFLVKKKSPEIGLATVYRTLEILTELKIVDKVSFNDGLARYDLRKEGAKHFHHHL
                     LCLNCGTIEEVEEDLLLEVEQVVEERYHFLVKDHRLTFHGICQECYEKEKRNQSV"
     gene            complement(63884..64747)
                     /locus_tag="ACG842_000065"
     CDS             complement(63884..64747)
                     /locus_tag="ACG842_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor B family protein"
                     /protein_id="HFQ0246638.1"
                     /translation="MNDLLATIFTGIIIDENEKNYFVQKNGQTFGLSKSEGEHKIGEA
                     VEGFGYQNQKHENRITTQIPKSRIGHYAFGIVTATRKDLGAFVDIGLPDKDFVVSLDE
                     LPTMTELWPKKGDRLMISLRVDAKDRIWGSLADEKIFKSLSKYGTEEMKNKDVIGTAY
                     RLKLTGTYVLTEDFYLGFIHPSERYNEPRLGEQIKGRVIGVRPDGVLNLSLKPRAYEA
                     ISDDAQMILTILERSADQQINFTDKSDPEEIMKAFGISKGAFKRALGNLLKQGLITQE
                     NGVTKLVKKSN"
     gene            complement(64943..67042)
                     /locus_tag="ACG842_000066"
     CDS             complement(64943..67042)
                     /locus_tag="ACG842_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division site-positioning protein MapZ
                     family protein"
                     /protein_id="HFQ0246639.1"
                     /translation="MKKCPNCGSKKMEQNTICPDCGHQITAEDQQIKESIEETNTDAA
                     MQDKSTEDAVFPDTELNDPIEWSELKDLPLESVMELFEKAEPEDEEPPAHDQVTNIEK
                     NEQKKATVHETSEESGKNQEETLESKQKRRVAELKEIVDNEEENSILSAYIKAHREDT
                     KEEHAKELLKMISEKIAQENKVEPEKEQETEKDSDRENATGKETVKIKDVKTDITEKT
                     EVFTEKQLEKEKTNKLPEAEKKEILENTDKKSTEPDEINSESDAENQSVSKEGTDLEA
                     KIEKEENQPKLFEAEAKSITIVPDTEKTHQESNLEKTDPDKAQKKKSKKVPYLVLAAV
                     LLLGGGSWAYYDHHQKVEAEVAAEAKRQQQMEELHEKLAAFYTDDHHQFIRADMIHQD
                     LSKLKNDLSSLKNEKGYSELETTYQDIQSKIRDIQKVNEWFIAPAIVDDHLADDIKLK
                     ADKEIQAIETDNTDFGKLIQQAQTEAKNQYAELQTAKEKTAIVFKDGKVVDSATRDQY
                     KTAKEAVEKVKNTDLSAQLNEQLKQVDKMLTDKEKKEEEEKKKAEEAKKAAEEQAKQA
                     AQAAAAAQAAQQAAVAQAAQQEAASSTPSSSNSANQPIKSARASDIADASNPAWTWAP
                     GVQSKVIATCIQRGYIVEGGYRLEKARIENGEGYYNLYATSTKSALMKGIGESALPFY
                     IVTINCKTGWFGGNGSN"
     gene            complement(67262..68371)
                     /gene="rpoD"
                     /locus_tag="ACG842_000067"
     CDS             complement(67262..68371)
                     /gene="rpoD"
                     /locus_tag="ACG842_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HFQ0246640.1"
                     /translation="MANGTDKKTYEAAVAAFIKENKPKRQVVYDDLSNQLATPFTLNA
                     DEMDKLIQKIEDAGISVVDENGDPSIHSLKSAEKKAEQAKTEDLSAPTGVKINDPVRM
                     YLKEIGRVSLLTAEEEVALALKIEEGDQEAKQRLAEANLRLVVSIAKRYVGRGMQFLD
                     LIQEGNMGLMKAVEKFDYRKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETIN
                     KLIRIQRQLLQDLGREPTPEEIGAEMDLPTEKVREILKIAQEPVSLETPIGEEDDSHL
                     GDFIEDQEATSPAEHAAYELLKEQLEDVLDTLTDREENVLRLRFGLDDGRTRTLEEVG
                     KVFGVTRERIRQIEAKALRKLRHPSRSKQLKDFLE"
     gene            complement(68395..70236)
                     /gene="dnaG"
                     /locus_tag="ACG842_000068"
     CDS             complement(68395..70236)
                     /gene="dnaG"
                     /locus_tag="ACG842_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HFQ0246641.1"
                     /translation="MAQRIPQEVIETVRSQTNIVEVIGQYVQLKKSGKNYLGLCPFHE
                     ERTPSFSVAEDKQIFHCFGCGKGGNVFTFLQELEGLSFPEAVVKVADFEQIPLEDQWR
                     QRTIAVRNPESATGKLIAAHEKAAEIYHHMLLHTKAGQPALEYLQSRGLSLELIEEFN
                     IGFAPNERSFLQQVFKNESISNELLERSGLFVTHDDGRMSDRFYQRVMFPLRNSQGQT
                     IGFSGRWLAPEKGQEKDQPKYLNSPETELFNKRQVLFNLDKARSDIRKNGEILLFEGF
                     MDVIAAWQAGIKTGLASMGTSLTAQQIQQMEKLTKELVFCYDGDNAGFEATNRGIELL
                     RQNSRLALSVVVVPEKLDPDEYLRKYGEASFQELVAHGRETVFTFKSRYLKQGKNLDN
                     EKDKIEYLEELLVELSRVVSPIEQDMYLSQLSTEFQIGRETIQKQLRDLRRTNQQNRQ
                     EPQESQHTQVTRQETARKKRPLTQVEKAEQILLYRAFKEVSVRNILKEREFQFIHDAY
                     AEVYFLFDAYVSQHYEFNLAEFLDFLQDENLRRLVVEIEYLPVAEESSPREIQDLLDV
                     ISKSGLADEITLKKQMQQEARRTGNKQLELELTIEIINLTKQLKQAK"
     gene            complement(70418..72733)
                     /locus_tag="ACG842_000069"
     CDS             complement(70418..72733)
                     /locus_tag="ACG842_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HFQ0246642.1"
                     /translation="MNGLTQQEVVQKMASGQQNDYQEDASKSTKQIFSDNILTLFNFL
                     NFGIGICLLFVGAYSNMAYLAIIVVNIVIGIYQEIHARNLVRKLSIVSQSEVLVIRDG
                     KEQSISTKELVLGDLVKIGAGEQIPADLQVISGRAEANEALLTGESDLIVKETGAELL
                     SGSFLTSGSVMAEVIRVGADNYAVRLTSEAKTHKAIRSELVDSIRKVSKFTSFVIIPL
                     GIILFLEALFIRDASIKISVIASAAALLGMLPKGLVLLISIALTTGVTKLAKKRILVQ
                     DMYSIETLAHVDTLCLDKTGTITEGKMSIQRVEALHPLYEKAIPEIMGTYLAESSDNN
                     ITMRAMRDYFELSNRYDVTNKVAFSSDRKWGAMEFPELGVVYVGAPERLVEEDRLPDS
                     LKTAQANGYRVLMLGIAPNQQLNEEKPAFVEPVALFEIDDPIRKNAQETLAYLHEEGV
                     DLKVISGDNPFTVSNIARRAGLPNYDAYVDLSQITEEMEVREAAHRYTVFGRVSPQQK
                     KLLVNELKESGRTVAMTGDGVNDVLALREADCSIAMAEGDGATRQIANLVLLDSDFTT
                     LPEVLFEGRRVVNNVTKVSGIFFIKTIYSFILSIICAVTAIGFPFIPIQITLIDLAIE
                     GYPSFFLSFEQDKRKITYRYLPTALINALPNALLVVVNIIAVYLLGKTFAWGNAETTT
                     LMYYLLIGISCMAVVKACYPFNPLRIFLAVTTIVGIYVAAMLFHGILEVNLGLGESVG
                     YFFVFMLVNIVLRVLYWKADIQNQLLKRVAN"
     gene            complement(72918..73778)
                     /locus_tag="ACG842_000070"
     CDS             complement(72918..73778)
                     /locus_tag="ACG842_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HFQ0246643.1"
                     /translation="MEKRQKMENQEVGYTTKDWMLRFVKGMFIGSGFILPGVSGGALA
                     AVFGMYERLISFLAHLTKNFKENVLFFLPVGLGGVTGIFLLSFLVSFLLGAYETTILW
                     FFVGCIAGTVPALWKEAGKKGRDTSDVIIMVVTFVLAYLFLLYGAQLFDGQVDQNFGT
                     WLIAGGLIGLGMIVPGLSPSNFLVYMGLYKAMADGFKEVRLEVIVPIGIGGILCVLGL
                     SKIMDYIFSKAYSKLFHFILGVVFASTIMIIPTDYAGLGVAGVLACPVLFIAGTALGW
                     WMSRLEEQYK"
     gene            complement(73837..74469)
                     /locus_tag="ACG842_000071"
                     /pseudo
     CDS             complement(73837..74469)
                     /locus_tag="ACG842_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289460.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme family protein"
     gene            74636..75397
                     /locus_tag="ACG842_000072"
     CDS             74636..75397
                     /locus_tag="ACG842_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFQ0246644.1"
                     /translation="MTDIIVILYTVGDYVKKEKKYAKMPDGSEIYYEKSGQGFPLFLL
                     HGNDGSGRFFSEQVPVLERYYTVYLVDSRGHGRSTNEASMLNFQLMAEDLNTIMLLEK
                     IDQADFLGFSDGANLALVFASSFPKKVHRLILNSGNTLVKGVRFSARVISNIHYAWVW
                     LLSLFRPSLRKNLLVIKLLLHDIGLTENDLKKINSPTLIIVGKKDVIKLKHSLYIAKT
                     IPKASFVLVKEQGHELARKDPERFNREVLQFLSET"
     gene            75423..78014
                     /gene="mprF"
                     /locus_tag="ACG842_000073"
     CDS             75423..78014
                     /gene="mprF"
                     /locus_tag="ACG842_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HFQ0246645.1"
                     /translation="MLKNTIQWLKNHLGLFKTIFLISVIVIIVGELMSIGKTLSIQQL
                     GETFATIPVWKSGLMLLIGLISVLPMVGYDIILNRMLEQKQNPRYLFETSWLINTINN
                     IAGFGGFVSVGLRSELYGQKKESRNVIQALSKVFLFLMAGLSIYSLLSFLLVVFTPVA
                     PFLKQYWIWLIGGGLYFPVVFLFTTFKKKGFLGGTSLKTQGQLLLVSLLEWTGVLFSF
                     LSVGYLMEIRIDLWQTIPLFIAASVIGIVSMIPGEIGSFDVMMIIGLSAIGVPREIVV
                     VWILLYRLFYYIIPFLIGIVFFFKNIGSTFDQRYSGIPKQLATEIAHKIVVVLLYFSG
                     IMLVLSATIPQAFTEFRWLHSLNPLKFHFIIQFPSILLGFLLIIMGRGIAARVKRAYL
                     PTIFLIVLALLYVLLSDFSFTPVVFLSILLLIILASKNELFREQLIYSWEWRTIDGII
                     IGALSLLYIVIGVYNLPDFPHRRHHFISFFLFPSEKIWFSGLLAIIAVSFMIVLFVHF
                     LQGEKKQIGEAFNEEKALKILTTYGGNSDSQLIFLKDKRMFAYEKDGEPTVLLQFACF
                     NNKCIVMGDPSGKKEDFPEAIEAFIEETDRLCYLPVFYETSEEIVMILHEFGYDFIKM
                     GEEAYVDLNSFTTSGKKMKGTRAVLNRIEREGFTFDVLQPPFSTEQMSTFKNISDNWL
                     GSRKEKGFSLGFFSEDYLQRAPIAVVKNTQEEIVAFATIMPTYTNNKVGTIDLMRYDS
                     KKAPSGSMDFLFLHLFAYMKEEHIQWFNLGMAPLSNVGTSRKSFLQERIAYLVYEFGS
                     HFYSFHGLREYKSKYATNWVSRYTLYSRDSWIAYVMIALLIIDNAPVEQTQGRISGLK
                     KWIRRKY"
     gene            complement(78202..79389)
                     /gene="tuf"
                     /locus_tag="ACG842_000074"
     CDS             complement(78202..79389)
                     /gene="tuf"
                     /locus_tag="ACG842_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014335040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HFQ0246646.1"
                     /translation="MAKEHYDRSKPHVNIGTIGHVDHGKTTLTAAITTVLGKKGLANP
                     QDYASIDAAPEERERGITINTAHVEYETEKRHYAHIDAPGHADYVKNMITGAAQMDGA
                     ILVVSATDGPMPQTREHILLSRQVGVKYLIVFLNKVDLVDDEELIDLVEMEVRELLSE
                     YGFPGDDTPVIKGSALKALQGDPDAEAAIMELMDTVDEYIPTPERDTDKPLLLPVEDV
                     FSITGRGTVASGRIDRGAVRVGDEVEIVGIKPETQKAVVTGVEMFRKTLDYGEAGDNV
                     GVLLRGIQRDDIERGQVLAKPGSITPHTKFKAEVYVLTKEEGGRHTPFFNNYRPQFYF
                     RTTDVTGTITLPEDTEMVMPGDNVTIDVDLIHPIAVENGTTFSIREGGRTVGSGIVTE
                     IEA"
     gene            79757..80113
                     /locus_tag="ACG842_000075"
     CDS             79757..80113
                     /locus_tag="ACG842_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HFQ0246647.1"
                     /translation="MKKLIGVLVVLGIFLGGSFAAYHYFYGGEAYYTKITTNGEMSAT
                     QADNGEKFTTYTYKQAAYNKDGEKKQVTLREEREHPLKMNAYLKLKVNPRKGVLSWEE
                     IKASEVPKNAAEKIDN"
BASE COUNT        21979 a        16847 c        13741 g        27576 t
ORIGIN      
        1 atcttcttta taaaaaaata aactattttt gcaaacgatc tgcgaaaata gtttgttttt
       61 ttattttgcc attattttct tacttattgg gagaaaaaat tcgcttaaac tcaaaaaaat
      121 ttgaggatac gatataaact ttcggataga gtataaaata aggaaacgcc gcagcgtttc
      181 ctttccaact gtagccgaat ataaatttgt atatcactta tttaaaatag ctattttctc
      241 ccaaagagtt tccttgatca tcgatcaatc cttgtaatga gcgttctaag ataccatggc
      301 tttttttcga ttttttttct tctgactgct ttgtttcctc taacgttttt gttcgattga
      361 atgttcgtac agacggctca ccatttcttt tttcttgata cggcgtcggt tctgtatcga
      421 acaacagata agagtcatcc gtttttttca tcgattcgat taaatgatcc tcagaaattt
      481 tgctttctct agggtctggg ataatcgatg cgggtacata tttaggaaca aaataagaac
      541 gctcccgcac cttcgtgtca acagatgcgt ttcgtgtagt atgcttggac tctgtccttg
      601 tttcactttg tctgacagaa cgagaggaat caccaaaaag tttttttcgt cctgtcgatg
      661 tgatttctga ttggtaatgg ccggtataat ccggtaattt tcctttatgc ttttccaaat
      721 tttccttacg cggagctgac ttcaatccat ttgttgcacg acgcttgcga cggattttct
      781 cggtgttcgt gttcgtgaaa tgaaattctt tcatctgttc aaacggggac tgattctgtt
      841 cagtcaaaag atcttcttgt ccgtcaaata atactcgctt cttgtcgttg gttaatttta
      901 cgcctaactc atcatcgtac gcaggaaaac ggaaatgttt tcgtctgatt tctttcattc
      961 aaaccatcct tctcaaaaat ggaacttttc tcaataatag cacagattca ttgttttcag
     1021 tagcaatgcg ctgattattt taagaaaata gcgatgaatt tcataaatat atatatagta
     1081 ataagactta attcgttgtc tctctgataa ctagttcagg aaaaaaacga cgataacctt
     1141 gactcgcccc tttttcttct atttttttta acaacatttc tccacatact tcacccatac
     1201 gtataatatc ttgcttgatc gttgtcaatt taggtgaact gatttggtct aaaaataccc
     1261 catcaaatcc aatcacacca aattcttcgg gcaagtgtcc tccagcattt ataatccctc
     1321 ttactgctcc tagtgctagc cgatctgaac tacatacaaa acaagtttta tctgtaaaat
     1381 ctgcccagtt ttctgtcata tatctttcta cttctgtcga atgatttgga aaccgcaaga
     1441 ttttactagg aagatcaact tccttcatca cagataaata accagtttcc cttgaaattt
     1501 caaaaggctc gtccacttcg ataccgatat aaataatcgt ttgataatct ctttctaagg
     1561 catattgcgt tgcttttgct gtaccatatt cattatcgct gtctacataa tcatatcctt
     1621 cagaattttc cccaaacaag acaactggtt tttccaaacg gtcgatccaa tcgaagtctt
     1681 tttttctgac acctgtgatg atgtatccat cacaagaacc gacgtcgaag gcatcatttg
     1741 taactaattg gagagaataa tgcgctttac cgatccgttt agcgattccc atcagcaaat
     1801 tcatgtaata gggctcagtt gtgtcgattt cttccagtat aaataatttg atgatctgcg
     1861 accgattcgt gacaagtgct tttgcagcaa tatttggacg gtaatccagt tcagccattg
     1921 ctgagaaaac taactctttc aattcgtcag tcacctgttc cggatgattg atgactcgag
     1981 agaccgtcat ttttgaaaca ttggcacgtt ttgcaacatc tgctaaagtg ggcatgctct
     2041 cttcctttct aacatgttgt cattcttact atcaattata ccaaattttc ctaacagagt
     2101 ttattatagc ccaaaataaa aaacggaaaa tcctctatcg tcatgagtga ttttccgttt
     2161 caattattta tactttttta tcaaattttt tgctaaaaaa gtggaagctt cttccattag
     2221 tggtatttgt accatatgtc gctgtgtttt tgaggaacga aaaacaattt cttttccgta
     2281 agacttatgc cgattttttt ctacaaatcg ttccgtttgt atataaggga ttttttcgtc
     2341 agcttgtcca tgccaaaaga acagaggacg tccagctaac ttttccggct gcaatgacaa
     2401 atcatagctc ttaacccatg aaatcaaatc aaaatagtct tttggtacga cataattata
     2461 ttccgatgcg cgccttacga tttcttttcc atattccaaa ggtgcagggg aacccatcaa
     2521 acacacgccc gctactatat gaggatgttt agccaacaag gcgcatgttg tcattccccc
     2581 catcgaaacc ccgccaactg caagctgatc atgtagaaga cctctttttt ctaaatgctt
     2641 gataagtgtg tcaaattcaa acagatttcc gtagatacta tcccaaaatg tataggatgg
     2701 aatagaagaa accggctgtt tgcgctcccc atgattcatc gcatctggca ggatcacacg
     2761 aatgccctgc tgcgcaattt ttcttccttg cgtcaaaact aactccttac ttgtctgcca
     2821 tccgtgataa tagatgacaa cgggaatacg ttcctctctt aattctttca gaacaacttc
     2881 taacactgga atatgatgga cagaacgttt caatataatt ggattcaatt gttactcacc
     2941 tattttcatt aaatttcctg atcgccatag caaccatgct ccaacagttg tctggaacag
     3001 gttcagtgta ccaaataata taaatgcagg actgaatggc tcgggaaata agcacagaga
     3061 acaaagtccc accactccta atgctagcga aaggagtgga taaatagaaa aattcaaaaa
     3121 actggcaacg ctaccgaaaa tattaccaat aaacagtatc aaaaagaaaa aaacgcttaa
     3181 aattaaacca tctgtcagcg acatctgaaa aacaatgttc tctacctcaa ttggctgttg
     3241 aatcgaatag acacctaagt atatactgag aaggagactt agcacgtggc tggcacaaaa
     3301 cacacgattg accgttttca ttttaatcac ctaacctatc tttataattc tattataatc
     3361 attttatcat ttttttgtat gtataaagtt tcaatttttc ttcacttacg attttttttg
     3421 atattgttgt tatataaatg aaaggagttt tacaaatgat tgatccaaaa atgttgggga
     3481 tgatcttcct cattaatttt gcttatatca ctctgaacac gttacgtttc atgttgacaa
     3541 tgaaaggcta tcgactgatt gctccacttg tcagtatggt cgagatcacg atttatatcg
     3601 tcggcttatc ccttgtttta gatcgtttag ataatccact gaatttattt gtctatgcgt
     3661 taggttacgc agtaggtatc agtgtcggaa ttttgattga agacaaattg gcattaggat
     3721 acatcatggt cacaacgatt ttgccagcaa atagcgcaga agataaaagt ttgccgggta
     3781 tactgagaga acacggttat ggtgtgacac agacttctgc aatggggctg gaaggtgagc
     3841 ggctagttct cgaaatactt tctcctagaa aaagcgaacg tgaactgtat cgcctgatca
     3901 aagaaatcga agagcgtgct tttatcattt cttatgagcc aaaatatatt tcaggtggtt
     3961 tctggactaa aaaagtgaga aaacgacaag gaaaataact cacaaactta aaaactgtct
     4021 aatatctaaa ttaaatttta aatgttagac agtttttatt tattattttg attaaaaaac
     4081 atcgacatat actcatcaat ttgttattat actttatata cttatcaatt taatttggag
     4141 atattatgga aattcaacgt agaaccacta attttttaca tttaggctct ttttttgcaa
     4201 ctttgacgat cttatttttt ctatgttatt cataccttaa gacctcacat gcgccttcta
     4261 gtgcattagg ctggttgctt ttggatcatt ctgatatcac agaagccgaa gaaaatccgt
     4321 tttttttgat tttgtcttct ctttgcggct tactgttttc tgtttatttc ggtgcctatt
     4381 tttttcatcg acatcctggt acgttaaacg aagcttcaag cgtattgatt gcatttaaaa
     4441 cgttttcttt tttagcaatt cttttttatt cgtttaatca caatacgctt ttcttcttta
     4501 tcatgaatgg tttagtcgtg attttatcca tttttactta ttttattttt tcactcattt
     4561 ttagccaatt gaaatgtccg ctcttctttc gattagttcc tgtcacctgt tttatttaca
     4621 gcttgttttc cttttttgtc cttgctttaa tgccactgac caacccttcg acgatccaag
     4681 tcgcaggaaa tctgttggat atgctcttca tcgtcagtct aggtgtattt atgtggcaaa
     4741 gaaaaaacaa acaaaataaa aagcattttg aagcctgact attacgactt cataaatgct
     4801 tttttagttt taaacacttc aatctaaaag aataacccaa gattggataa attattaatg
     4861 actcgccatt cgtttcttag tgattctgga ttatcaaatg gacttcctaa tctaatcagc
     4921 tagttcttcc cataaaatgc catacttgac tgaatcataa tattgattat ttaaaaatct
     4981 aacttttctt atcactactt cctttgtcat tccacatttc tcaccatttt tttgcattcc
     5041 tttgtttcca gaccatgtag tgaatctaat aggtggtaga taggcaaaat actcaaataa
     5101 ttcttttaac cataaaggaa gagaaaggct cctcctgcaa gagtcagtat aatttttgct
     5161 tactgctctc ttaggtttta ctcactaaaa ctgttccata ttctttgata taagaaaatg
     5221 catgcggtat aaatttcttg ctcagctcag aaaagatttg tcgtttaaga aaggctattt
     5281 cctccattgt caatgaagga tccaatgttg gtaattcaat ctcaaaattg tttttttcga
     5341 caataggtaa ttctaatgtg aatgattctt cttcctttat agtattcgtc acaagtagag
     5401 actgatggaa aatactaggc cggcgatttt tatatttatt cttacgtatc ctgtcaacga
     5461 taacttctat ttgatcaaaa aagtacgatt catttacgtc tccaataagt tcggctaatt
     5521 ctttgaatgc cttcgttaca ggaagctgat tcttctgtac aaaaaagtaa ttagccaaag
     5581 taagcgggaa gtgttctata gaggaaagtg tgataaaacg gtacttctca tttcctgcag
     5641 ttgtcctcag caattctgtc tctcggctgt ttcttactcc ataaaaatac gcttctttcc
     5701 gttcatcttc gaaacaatac gcccaaataa cttctcccat cgctaatttt tgttcatcaa
     5761 cagagagtct cttcaattgt tcagctatgg tttcagcagc cgaagtggct tgttgcaagt
     5821 aatctttagg atcaaatatt ggaaagttca aatgatatct tcgttcttcc ctcttgatta
     5881 atcctgcatc gatcaataaa tcaagaaaat gggaaaattt attttgcgaa aattcttttt
     5941 tcaattctct tagaatcgtt tcttgttctc gatgttcata tagataatcg gtcaatgaag
     6001 aaaaaagcgg atcatcggtc agtttttgat aaaagttttc cttatttttt gaataatagt
     6061 tcatatatgt tctcttttct atccgtttta ttttacttaa aaggctttta cgtaaaaagg
     6121 aggaagaaca agagtcataa ttgactgtta ttctcctcca tttcttcggc ctttagtatg
     6181 ttctatgttt ctttttatat caatcgctta tccattcatt ttgaatgctt catactacca
     6241 tttgattagc taaatacagc cgtcaattga ggtacgactt gttttttacg tgaaacgaca
     6301 cctttaagca aagcacggtt gttggtcaaa gttgtttgga aagcttcttc tactttttct
     6361 tttcctttac cagcaacaag taattctgaa tcactgtcta agatgtttgt gacaactaat
     6421 acgaacagat cgtagccatt tgctttcatt tcatcttcca ttgctgcttc tagctctgct
     6481 tgtcgatcca tcacttcatt aaggtccact gtattgactt gagcgatacg aacgttggaa
     6541 ctgcccattg ggaaactttt tgcatccaaa tcaagaagaa ccgcagctga tttatcactt
     6601 agatttgttc ctgctttcaa catttcaagt ccataagtgt ttagatcgac ttcagcgatt
     6661 gctgcaagtt cttttgcagc tgcttcatct tcaggcgtac atgttggtga tttgaacaac
     6721 aatgtatctg aaatgatagc tgagagcatg attcctgcga tttctttagg aattgttact
     6781 tggttttctt tatatagttt taaaataatc gtacttgtac atccaactgg ttctgcacga
     6841 tagtacaatg gattagctgt ttcaaagttt gcaatacgat ggtggtctac tacagctaaa
     6901 atatttactt cgctgatatc tgaaatactt tgttggaatt cattatgatc cacaagcatg
     6961 acatctgcat tttctgcttt agctgactct actactcgtg gtgcagatag ctgaaagtgg
     7021 tctaatgcgt attgtgtttc ttcattaggt gtacctagag caactgcttc tgtttcttta
     7081 cctaattcat tttgcaagtg tgaaaacgca atggctgccc cgattgcatc tgtatctgga
     7141 ttttggtgac caaaaactaa aatttttgac atgtcattcg ctcctttgtg atttctccta
     7201 tttaataata gcaaaaaaac ggaaagaagc aatacttatc ttctttccga tgcgacctat
     7261 cgcgttaaat attttgtata ctcttctgta tgaagcaatc gtttagcatt ttcgacacgt
     7321 tcttttgtag gtggttcgat cccttctaat ggatatttca aaccaagtgt ttcccacttg
     7381 tattttccca tcgtatgata aggaagaatc tctactctgt ctacatttga caaggtttga
     7441 ataaagttgt ctagtcggat caaatattca tcataatcac ttcgctcagg aactagtaca
     7501 tggcgaatcc aaaccggttt gttgattttt gacaaatatt gagccatttc taaaatattt
     7561 tcattgtccc aatgcgttag ttttttatgc tcttcattat cgatgtgttt gatgtcaaat
     7621 aacagtaaat ctgtatactt catcaattct tgaaaacgtg aaaagaaagg ttcttcatac
     7681 gtaaaaggtt ttccgcatgt atccaatgta gtatgcatct tccgctcttt tgctttttta
     7741 aatagttcga tcaaaaaatc gatctgcaat agaggttctc caccactaac agtaatacca
     7801 cctttatctc cccagtaagg tctgaagcgt tctgctttat ctaaaagttc atctgctgtg
     7861 tattctttcc ctccgccaat attccaagta tctggattgt gacaaaattg gcaacgcatg
     7921 cggcatcctt gcataaacac gacaaagcgc agtccaggtc cgtctactga accgaaagtt
     7981 tcgatagaat gaacatagcc gattgttttt tcttccattt aagttcacat cttttctttg
     8041 atttcatttt aagtacaagt tagctgtcga agcgaagctt tctgaacaaa atttttcgca
     8101 attgccgctt cgttatgtgt aaaatttaca gggacggaac atacaagtcc tgcccctgta
     8161 aatcgtttct ttcaaataag catactgatt attcgatttt gatgcgatgg ctattcaacg
     8221 tcctttcgac gctaagtcga aaaacttttt tcacgaactt tttacattct gtcgtgtgaa
     8281 gttctcatca agacatctaa ttgttgttct cttgtcaagt cacggaattt aactgcataa
     8341 ccagataccc ggatcgtcaa gttcggatat ttcattggat tttccattgc atcacgcaat
     8401 aaatcattac tgaatacatt gacattcaaa tgataagctc cttttgagaa gtatccatct
     8461 aagacattac gcaagttttc gattcttgta tcttgttctt ttccaagacc tgaaggattg
     8521 attgtttggg tatttgaaat tccatccaat gcacagccgt aattcatttt agctgttgag
     8581 ttcaggctag ctaataaccc actttgttcc gcattgtaac ttggatttgc tcctggcgca
     8641 agtggttttc cagcttcacg gccatcagga gtatttcctg tatttttacc gtaaacaaca
     8701 tttgaagtaa tcgtcaataa tgaagttgtt ggttttgagt tgcggtaagt tttatgacgt
     8761 ttgatttgtc ccatgaagta ttccaagatc catgaaacta attcatctgc acggtcatca
     8821 tcatttccgt atttagggaa gtcgccatcc acttcaaaat caactgctat tcctttttca
     8881 ttacggattg cttttacttt tgcatatttg atcgctgaca agctgtctgc cgcatgagag
     8941 atcccagcaa tacctgtagc aaatgtgcgt tgcaagtcag aatccatcaa agccagctga
     9001 gctgcttcat aagaatattt atcatgcata taatgaatga tgttcaacgt attgacatat
     9061 agttctgcta accaatccat catattcatg aagttatgtt tcacttcttc aaagtctagt
     9121 atttctgatg tgattggacg atattctggt ccaacttggg cgccagtcac ttcatcgact
     9181 ccgccattga tggcatataa tagtgtttta gccaagttag cacgagcgcc aaagaattgc
     9241 atgtcttttc cgacaactgt tcctgataca cagcaagcaa tcgcacaatc atcagaaccc
     9301 cattgttcac gaagctgatt atcattttcg aattgaatcg aagaactttc gatagcgatt
     9361 ctgctagcat atgttctgaa tccttctgga aggtgttcag aatacaaaac agtcaagttt
     9421 ggttctggtg aaggtcccat atttgttaat gtatgaagca gacggaaatc attctttgta
     9481 actaatgaac gaccatctaa tcccatacct gcaatggata aagtagccca gatagggaat
     9541 cctgagaata attggttata ttcaggtgta cgcgcgaatt tgaccatacg taatttcatt
     9601 actaagtggt cgatcaattc ttgtgcttct ttttctgtga ttactccatt atccaaatca
     9661 cgttggatat agatatcaag gaatgctgag atacgtccga ttgacatcgc agcaccattt
     9721 tgtgatttga ttgctgctaa atatccgaag tatagccatt ggacagcttc ttttgcattt
     9781 gcagctggtt tagaaatatc atagccatat ttcgcagcca tttctttcat ttgcgctaat
     9841 gcacgatatt gttcagtgat ttcttcacgt aaacgaatca cttcttcact cattgtatga
     9901 taaccagtca tatcatgatc ttttttcttt tgttccatca aatagtcgat tccatacaat
     9961 gccacacgac gatagtcacc gatgatacgt ccgcgaccgt aagcgtctgg aagccctgta
    10021 atgattttgt ttttacgagc agcacgcatt tcagcagtat aagcgtcaaa tacaccttga
    10081 ttatgtgttt tgcgccagtc agtgaaaata tgtgtcaatt catcgtttgg tgtatagcca
    10141 ttgcttttta gcgcattgtt agccatattg attccgccga acggcatgaa tgcttgtttc
    10201 aaaggtttgt ctgtctgtag accgacgatt ttttccaaat ctttatccaa atagcctgca
    10261 tcgtgtgaag taatagttga tacgatttct gtgtccatat catagacacc gttacgctcg
    10321 aattggatat catttaactt ttgcaattca tcccataatt tttctgttgc ttctgttggg
    10381 ccttccaaaa agctgtcatc accgcgatat tcagtgtagt tattttgaat aaagtctcta
    10441 gtgtctacgc cttctttcca tttggtacct ttaaatccat tccattgctc cattgtatgg
    10501 acctcctaaa tttctgtttt atgtaacagt ttttacgtga ttagtataac acagcaccct
    10561 atctttgcaa gggttttctt gttttttatt taacaatttt tatgttttat ttacatatat
    10621 ttaaatcaac gttgacatac caacatttaa gcattgtgaa cactttcatc tttttttgca
    10681 aacgtataag gatttattaa aaatgaatga aaaagagacg ccacctgtat taatacagat
    10741 ggcgtctctt cattaaaata ctacaacagt tacgactcga tgaaaatctc aggagcatcg
    10801 ataacttcga tgacttctcc accttttttc tcatccatcg caaatgatcc attcgatgtt
    10861 cgatcaccta tagggaaatc agttgggtca atcgttaact gctgcccttt ttgattcatc
    10921 aaggttattt cttttgatct tccttccgac atatagataa tacggtgcgg attttttttc
    10981 agctcacgca tgaccgtcac tccccgcttc gcacgtgaag tttggctgat ttcttgagca
    11041 agcatgcgtt taacagctcc tcgctgagta acgattacga tcggcgtatc accttctgct
    11101 aacacaagca gcccgttgat aatcctatct ccatctttca aattcataga cttcactcca
    11161 gctgcctttg ctccaacaac gggaacatct gttaaaggat agcgcaaacc cattccttga
    11221 tgcgttacta agaaaacatc taagcctggc tgatcttctt tcagatacac agcaatcagt
    11281 tcatcttctt ccgcctttag cttcatacag ctgagcggac ggctcttata ggtgcgccat
    11341 ggttcgaact ctgtcattcg tgtttgcttg atcaatccat tcttgctgat gaatacaaat
    11401 gttttctctg cttgaatttt ttgatatggg aaaacagcta aaatcgattc gtctacagac
    11461 agattggaga ttgtttgaga aatgtgctca ccagcatctt tccatttcaa atcgggtagt
    11521 tcatggacag ggcggtagat cacatttgct ttatttgtga tcagaagaac atggtccaat
    11581 gtactcaatt cacctgagta tagcaagtag tcgccttctc gcatcccgat ttcttctggt
    11641 ttggaagcac tataagaacg aaggcttgtt cgcttcacat agccttctcg cgtcacactg
    11701 acaattacgt cttcttgtgc cactaagact tgcgtatcaa ttttgatttc ttcgatttcg
    11761 tcttcgattg tcgttagacg agcggaagaa taattttttt tcacttctcg caattctttt
    11821 ttcataacag agaaaagctc tttgtcattt gaaaggattt tattcaactc tgtgatttgc
    11881 gcaatcaagc tttcggattc cttttgaagc tctgtgatat ccgtgttcgt caaacggtat
    11941 aattgcaatg tgacaattgc ttctgcttgt tcttctgtaa attgaaagac gtcgactaaa
    12001 ttctttttgg cgtctttttt gtctttactt tcacgaatcg ttgcgattac ttcatccaga
    12061 atcgataaag ctttcatcaa tccttctacg atatgctgtc ttttttgagc tttggctaaa
    12121 tcgaattgac tacgttttgt tatgacttgc tttcgatgct cgatataact tcttaaaatg
    12181 tctttcagtc caacttgatg cggagtcata tggtcgatag cgaccatatt gaaattgtaa
    12241 ttgatctgta gctcagtgtt tttgaacaaa taattcaaaa ttccttgagc attggcgtct
    12301 tttttcaatt cgacgacaat ttgcagacct gttctatcac tttcatctcg tacttcagct
    12361 attccatcga ttttcttatt caaacggatc tcatccattt ttttcaccaa ggtcgcttta
    12421 tttacttcat atgggatttc tgtgatcacg atttgctgtt tattaccctt cattgcctct
    12481 atctttgttt tcgaacgaag aataactttt cctcgtcctg tttcataagc ttttttgatt
    12541 tcatcttttc cttgcaagat ccctcctgtt ggaaagtcag gtccaggaat atattccatc
    12601 aatttttcaa ggctcgcttg tggatgatcg atcatataga ttgtgccatc gatcacttca
    12661 gctaagttat gagtcgggat ttctgttgca tatccagcag atatacctgt cgatccattg
    12721 acgagaaggt ttgggtatcg agctggaagg actgttggtt ctttttctgt atcatcaaaa
    12781 ttccacacga gatcgactgt ttctttctcg atatctgcta gcatctctcc acttaacttg
    12841 gataatctgg cttcagtata acgcatcgca gcaggtggat caccgtccat actcccgtta
    12901 ttcccgtgca tttcgatcag tacttcccgc aacttccagt cttggctcat gcggaccatc
    12961 gcttcataaa tactgatgtc accatgagga tgataattcc ccatgatatt tccgacagat
    13021 tttgctgatt tacggaatcc tttgtcatac gtattgccat ctttgttcat ggcaaataat
    13081 atccggcgtt gcactggctt caatccatca cggatatcag gcagtgcacg ttcttggata
    13141 atatatttgg aataacgtcc gaagcggtca cccatcactt cttctaaagt taattcttgt
    13201 acttcttgtc gattttccat caggtgacct cctattctac ttcaaataac tgattgtttt
    13261 catttttatc tgctcgttct tcatctaata aatcattaga aactgaagga gagatttcag
    13321 tatcctcttt tttatctaag atactgccat cttcttcgag cgtaaattgc acgtgatttt
    13381 cgatccattt tcttcgaggt tctactttgt cacccatcag tgtggttact cgacgttcag
    13441 cttgcgcagc atcatcgata cggacgcgga tcaacgtacg ggaagtcgga tccatcgttg
    13501 tttcccacaa ttgttccgca ttcatctctc caagtccttt atatcgctgc agcatatatc
    13561 ctttgccgac ttttttaatc attgctgcca attcgtcatc tgtccacgca tattcaatga
    13621 cttgcttctt tccttgtcct ttggatactt tatataaagg cggtaacgcg atatatactt
    13681 tcccagcttc aatcagcggt ttcatataac gataaaagaa agtaagcagt aatacttgaa
    13741 tatgggcgcc gtctgtatcc gcatcggtca tgatgattac tttgtcgtaa ttacagtctt
    13801 cgatagaaaa ctcaggaccg actcctgcac caatcgtata gatcatcgta ttgatttctt
    13861 catttttcaa gatatcttgc attttagctt tttcggtatt gatgactttt cctcgtaaag
    13921 gtagaattgc ttggaacttt ctgtctcttc cttgtttagc agatccgcca gcagaatccc
    13981 cttcgactaa atacaattca tttttcttag ggtttcggga ttgtgcgggt gtcaattttc
    14041 ctgaaagaag agattcgcct tttttacgct tcttcccatt tcggctttct tctctggctt
    14101 tacgtgctgc ttctctcgct tctctggctt tgattgcttt acggaccaac atctggctca
    14161 tttcgctatt ttcctgcaaa taaaagccca tttgttcact gatgacgtta tctactacgg
    14221 tacgagcaac tggcgtacca agtttttctt ttgtttgccc ttcaaattgc agaagatttt
    14281 caggtacacg gatagaaagt acagcagcta acccttctct gaaatcacta ccttccagat
    14341 tcttgtcccg ttctttcagc aaaccaactt ttctcgcata ttcattataa gatttcgtca
    14401 tcgccgcttt cataccagct tcatgggttc ccccatcttt tgtccgaacg ttattgacaa
    14461 aagataaaac attttctgaa taaccgtcat tgtattgata agcgacctct acttcgatac
    14521 cttctttttc tcctgaaaaa taaacaactg gtgtcaatgt atctttttct tcattcagat
    14581 aatcaacaaa ttctttgatt ccttcttcgt aatgaaaaat ttccttgact ggttcttctc
    14641 cacgaagatc agaaagagag attttaacgc cttttagaag aaaggcagat tctcttaacc
    14701 gttcggctaa aatttcatat gaaaatttcg ttgtactgaa tattgtatca tctggtaaaa
    14761 aatggacagt cgtcccgttt gctttttttg tttttcctat ctttttcaaa gtaccgtcag
    14821 gtttaccgcc gtttttaaat ttctgctgat actctacgcc atctctaacg atcgttacaa
    14881 ttagccattt cgataaggca tttaccacac tggctcctac accgtgcagt ccaccagaag
    14941 ttttgtaccc tccttgacca aactttccgc cagcatgcag cacagtaaaa atcacttcaa
    15001 ctgttggtat tccggaagca tgcattccta caggcatccc acgtccgcta tctgcaacag
    15061 ttatactatt gtcttcatgg atggtcacat caatctcact gccataacct gataatgctt
    15121 cgtcaactgc attatcgacg atttcataga ctaaatgatg caagcctcgg ctgtcagtgg
    15181 atccaatgta catccctggt ctttttctaa ctgcctctaa tccttccaat acttggatgg
    15241 acgagtcatt gtattcgttg tttacttttt ttgccaaggc aaacgctcct tcaataatac
    15301 tttgcggcaa aaccgcacca cgattatcat actagaaaag gtcgtgaaaa aaaagagggt
    15361 agacgagaaa tcaattttaa ttatctccta accgcttttt cgacattccc ctctatcgat
    15421 catctcttct ttgtggtcat ttttattttt ggttttcttt caacttacca agttcaatct
    15481 taatacaacg atccattacg atatctgtac gtcctgcttt tcttagcatc tcagctgctt
    15541 ctttgttttc aatccctaat tgtgcccaga acactttagc atcagttttc aaaaaatcac
    15601 ctgctatttc aggtaaaaat tcactccgtc taaatacatc tacgatatcg attggatcat
    15661 cgatttctgt caaattcgca tagactttct cgcctagaat ttcttcacca gccaataatg
    15721 gatttactgg tatgatttta tatccattgt cttgcaatac ctgtgcaacc atgtaacttg
    15781 ttcgttctgg tttattgctc aagcctacga cggcaatatt ttttgcttct tccaaatagt
    15841 tgaaaatcgt ttcttgtgtt ggatttttta cagacatttc tgccacccct tgcttttctt
    15901 tttattccat ctattatact tgtatctttc attttttcaa ttgttcctgt tacctattca
    15961 tgctaaaata aaggatagtt ttcaacttga aagagggtaa aaaatgaaat tgatcttatt
    16021 acttatttta gcatatcttt taggttctat tccttccggt gtctggattg gcaaattgtt
    16081 tttcaaaaaa gatatccgac aacacgggag cggtaatact ggaacgacca atacatttcg
    16141 tgtattaggc aaaacagccg ggattgttgt cctgcttatg gatattttaa aaggtacatt
    16201 ggctacttgt ttgccgatga tcttccacct ttctgggatt aaccctcttt ggtttggggt
    16261 atgcgccatt cttggccata catttccgat ttttgctggg ttcaaaggag gaaaagctgt
    16321 tgcgacaagt gcaggaatgc ttttaggatt taatcctgta ttttttgttt attcttgtat
    16381 catctttatc gttagtttat tttgtacaag tatggtgagt ttgagcagta tgatcagtgc
    16441 agtattgatc actttatcta cgatcgtcct tccttatgta gctcctgtga ttttggctaa
    16501 gcccaattgg ttactgacga tcattgcctt tttagtttct tcctttatct tttatcggca
    16561 taaagacaat atcaagcgta tcagaaacca tactgaaagt cgaatcccgt ttggtttgaa
    16621 tagcagcaag aaaaaatcct gatacttttt cattccaaag agataaaacg ggagtgtgac
    16681 aaatcgatgt cacactccct tattacttac aggatagctt ttttacacac cttggtaaac
    16741 ggtgttccct gctaacttct cggtcacaac ctcttacttc actgtgatgg aatattttgt
    16801 cttgaagatt tcatgggcac ctagttgatt gatccctact ttttccatca agtttccact
    16861 actgttcacc gtgtcagcga ttccgcacca cggttcgata caaacgaaag gcgcttcttt
    16921 tggataagga gaccagatcc ctacataagg catatcttta tagcttaaag ttacgctatg
    16981 ggaagatttt tcacttcgaa tggcgtacgc atttagaccc cttgtttcaa aaaccaaggc
    17041 atcttgctca aaaagttcac gattcaaagc cagattcgta tttgtctgac ctaacgtctt
    17101 ttgatccata tcgatgaatg ggcctttcaa tggtaggcgt aatcttgatt tcaatggaga
    17161 aaatgccaga tagaaatctt caaatgacaa tccttcttca agaggtacat tgaaagcagg
    17221 atgtccaccg atagaaaaga acatttcttt ttcaccggta tttgccactt gataatgaac
    17281 cgtgatgccg tctcctccta attcatagct gatcactaat tcaaaatcaa atggatactt
    17341 ttctaatgtc tccggcgtac tccttaagat gaaactagcc atttcttgtc catgttcaat
    17401 gacttcaaaa tccatatccc gagcaaatcc gtgttgcccc atttgatatg ttttcccttg
    17461 ataaacatac tgatcatctt ttaaacgtcc aacaaaaggg aataaaaccg gtgcatgtct
    17521 gccccaatac gctggatcac cttgccagat atactctaaa ccatgttctt ttgatttcag
    17581 actgatcaat tccgcaccgt gttcagcgat ttcggcgatc agctggtcat tttgaataat
    17641 aacagtcatt gccattcctc caacttcgac ttacttttta aaatgggaag gctggaacaa
    17701 acatttctat ctgccccagt ccccttactt ctaataactg tgttttgcta actcgtcttt
    17761 aaaacgggaa ttcaatactt ttaaataagt tcccttcatg cctaacgaac gagattcaat
    17821 gattccagct gattccaatt ttcgtaaagc attgacgatc acagaacgag tgatgccgat
    17881 actatcggca atattagaag cagtcagacg accttcatct ccatccaatg cttcaaagat
    17941 agcttgtaca gccttcaatt cgctataaga taatgtgttg attgccattt ggacagccgt
    18001 tgcactccgt acatctgctt cgatgcttcg agacttctga taaaggatct gcataccgac
    18061 aaccgtagct ccatattctg ctaagactag atcgtcatct gcaaacgatt gttccatacg
    18121 agataagata atcgtaccca aacgttcgcc agctccaaag atcggaacga cagtcgtcag
    18181 accaaatgga tatttctctc ttgactctac cggaaatgca gtaatatcac tagtaattgg
    18241 gatattagct tcagttttca tcaactgtgc cactgtatca gtataagatt caggaaattg
    18301 tttctcaaca aacattgttt tgattcgttc attgttcaca tcgtgacgtt cattataacc
    18361 tagtaatact ccgtcacttc ctatgatata ggtgttgctg tcaagaatac cacctagtgt
    18421 gacagccatt ttatcgtaag gcaaatctgc ttgcatgtca aaagtattct tttgctgcaa
    18481 aagtgtgttt atcttgcggg ttttcgttag caagtctgtc attgtcgtcc tccgattttt
    18541 tataaaatat agcgacttaa atcttcattt gccgcaattg agccaagttt gtcatccaca
    18601 tatgcttctg tgatcgttat ttctcccatt tgcatatctg gtgcttcaaa taatagatct
    18661 tctagtagtt tttccaaaat ggtatgcagt ctgcgtgcgc cgatattatc tgtgtcacgg
    18721 ttgacatcga aagcgatttg agcgatccgt tcaattgctt cttttgtaaa cgtaactgac
    18781 acattttctg taccgatcaa cgcgacgtat tgttttacta aagcattatt tggttcggtc
    18841 agaatcttca caaaatcttc agctgtcaaa tcatctaatt ctacccgtat cgggaagcgt
    18901 ccttgaagtt ctggaattag atcacttggt ttagacaaat ggaaagctcc tgaagcaatg
    18961 aataggacgt gatctgtttg gatgatcccg tattttgtgt ttacttgtga accttcgaca
    19021 attggcaata tgtctctttg gacgccttct ctggatactt ctccagattg ctgtgattta
    19081 gaagtgatct tatcgatttc atcgataaaa ataattccac tttcttccgc taatttgatt
    19141 gcctcactat ggatatcagc atccttgact aattttgctg attcttcctt gatcaataac
    19201 tctaaagctt ctttgactgt taccgtccgt tccacttttt tcttcggtgt caaagcacct
    19261 agagtttcat ttaggtcgat ccccatttgc tccaatccat tgttcatcat tggtgcaggt
    19321 ttcttttggt cttcaacttc aatggttact tcacgactat ccagtaaacc cttttgtagt
    19381 tgttccaaga ctgtacggcg attgattcga atatcttctg tcacttcttc ttgcggttca
    19441 gcggtatttt gctgactagc attaaaaaga ttcatcattt gttcaaattg attagtggat
    19501 tgtttctgtt cttttttgat accaggtacc aagattttta ccaaacgttt ttctgcattt
    19561 tttttagcac ttgtgtatac acggctgtac tgctcttttt ctacgatttg gatcgcattt
    19621 tctactaaat cccgaaccat tgattctaca tctcttccga cataaccgac ttcagtaaat
    19681 tttgttgctt cgactttgac aaatggtgca ttgacgattt ttgccaaacg tcgtgcgatt
    19741 tctgttttac cgacccctgt aggtccgatc atcagcatat tttttggtgt aatatcttgt
    19801 tgcatttttt catctaactg caaacggcga tagcggttac gcaaagcaac ggcaacagat
    19861 ttttttgctg cgtgctgtcc tacgatatat tcatctaatt cacttacgat ttgtcttggt
    19921 gtttgtttta attcatacat atctactcac ctcttacagt tcttctacga taatattatg
    19981 attagtaaag atacagatat ctgcggcaat atttaacgca ttttctgcaa tttctttcgc
    20041 actcatgtcg ttttgagcat agtttttcat tgctcgggct gcagccaacg caaaattccc
    20101 tcctgatccg atagctaaga taccatcatc tggtgtgata acttctcctg tacctgaaac
    20161 aagaagcatt tctttatcat tcattacgat cagcatcgct tcaagttttt gcatagattg
    20221 ctgtgtccgc cattcttgag ctaattcgac agctgcgcgt tgtagattcc cgttatattc
    20281 gtttagtttg ccttcaaatt tttcttctaa agtaaacgca tctgcaacac ttccggcgaa
    20341 acctacaaca acttctccat tgtagatacg tcgtactttc tttgcagtgc ctttcatgac
    20401 aacagattca cccattgtta cttggccatc tccagccata gcaaatttcc cgtctttttc
    20461 gacggcacag atagtggttg aatgaaatgg tgattcgacc atgtttgttt cctcctaaat
    20521 ttgttctttt ccttattttt acgctcttgg atggaaagaa cgatagtttt tttgtaaact
    20581 ttcttttgtt acatgggcat agatctgggt agtcgataaa ttggcatgtc ctagcaattc
    20641 ttggactgtc cgcaaatctg ccccattatt taataaatga gtagcaaatg tatggcgcag
    20701 catgtgagga tggatatcac tatccaaact gctttttttg atcatttgat tcaagacata
    20761 ttcaattcca gtaggcgtga ttggttttcc atgatgattc acaaaaacga tatcatgctc
    20821 ttcgtgatat tttgtcatca atacctggcg gccttccctg aaatattctt gcagagcatc
    20881 ttgagcaaaa gaacctagag gagcatatct ctctttgttc cctttaccat ggatcaacat
    20941 gacctctgaa tcaaaatcaa tgtctgatac cgacaaactg ctacactcac ttaaacggat
    21001 gcctgacccg taaaggactt ccagtaaagc gcggttcctc aaatccaaag gactatctcc
    21061 tgctacgctg tcaaaaagtg cttgcatttc attttcataa aaaaaacgtg gcagtctcaa
    21121 atttttcttt ttgagatgaa cgtaagaaaa tggattttct tttatcactt cttctttgag
    21181 aagatactga taaaacgaac gtaggctggc tatcttgcga ctgatagaat ttctgctata
    21241 gctaggacga tgatcagcta atgcactcaa atagacacga accgtaaaat attccgcctt
    21301 cagcagatca gcgtctccag attcctccaa aaaatgtttg aaatggagaa tgtcttcttc
    21361 atatgcttct ttcgtttttt ctgaatatcc tctttcgaca atcaaataac gcaaaaattt
    21421 ttgttcccac gtggcttctg tcattttctc accttccttt tgtcccagag aataatttag
    21481 cacagcgttt ataaaaagac aaataaattg tcagaattta accaatattt ctaagtttgt
    21541 tcacaattgg cgcaattcct cacattttgt ttgagaaatt cttaagaaaa acgcaagaaa
    21601 aagagcgaga ctatccgtct caccctcttc atggagtttc cttactcatt tgggattgca
    21661 gttttttcta attctgtttt tattgcctca ttagctttta aagctcgttg tgcaattgct
    21721 tcatatcttg cttttttgtc tcgaatgcgc tctggcagtt caggaaacag accatagttg
    21781 gcattcatcg gttggaaatg ttttccttcc gcatgagtaa tgtagtaagc catacttcca
    21841 atagctgttt cttgtggaaa ctcgactggt tccatttctt ttgctaagcg tgcggcgttg
    21901 attccagcaa gtagtccgct cgctgcactt tcaacatagc cttctacacc agtcatctga
    21961 cctgcaaaga acagatcttc gcgtttcgtt gattggtaag tcggtttcag caattctggc
    22021 gaattcataa agctgtttcg gtgcatcaca ccatagcgaa cgaattcagc attttctaac
    22081 ccaggaatca tccggaaaac acgtttttgc tcgccccatt tcaagtgagt ctgaaagccc
    22141 acgatgttgt ataaagaagc tgcagcattg tcttgacgca gctggataac agcataagga
    22201 cgtttgccag ttttaggatc ttccaatcca actggtttca taggtccgaa aagcatcgtt
    22261 ttgaatcctc gctgtgccat tacttcaatt ggcatacatc cttcaaaata tttttctttc
    22321 tcaaaagatt taagttctgc cacttcggca ttgattagtg cctcatggaa agctttgaat
    22381 tcctcttctg tcatcggaca gttcaagtaa gctgcttctc ctttgtcata gcgggacttc
    22441 aaatatactt tatccatatc gatcgtagct ttgtctacga ttggtgcagc tgcatcatag
    22501 aaataaaaac catctgaacc attgaatgct ttgatgcttt ctgctaatgg atgagaagtc
    22561 aaaggtcctg ttgctactac ggtgattcct tccggtattt cagtaatttc ttcgtttatc
    22621 actgttacta atggatgctc tttgactttt cgggtaattg tttcagaaaa agaatcgcgg
    22681 tcaacagcta aggcaccacc agctggcact gctgtttcat ctgccgaagt gattactacc
    22741 gaattcaatc ggcgcatttc ttctttcaat aaccctactg cattagccaa actgtttcct
    22801 cttaatgaat tggaacatac tagttcagca aaattacttg tgtgatgcgc ctcagtcgat
    22861 ttgactggac gcatttcata taaacggaca gggactcctg cttgtgcgat ctgccaagca
    22921 gcttcacttc cggcaagccc tgctccaaca acatttacca ttttcaatga aagaacctct
    22981 tttctattat ttttggatgt cttcttcgta atctccattt acacagacta cttgtttacc
    23041 gcctttgact ttcttctcga ccagatatcc gtcacatttt ggacaatttc tgcccactgg
    23101 tttatcccaa gaagtgaatt cacattctgg gaaacgggaa caaccataga atagtctgtt
    23161 tttcttagat tttcgttcaa tgacttgtcc ttcatgacat actgggcaag tcacaccgat
    23221 ttcttttacg attggtttcg tattccgaca atcaggaaaa ttactgcaag cataaaactt
    23281 cccatatttc cctaatttta tcaccatcgg atgtccacag acatcacaat cgaatcctgc
    23341 tggttcatct ttgatttgaa ttttttcgat tttttcttcc gcattcgtca gttctttttc
    23401 aaacggttta tagaaacgat caaccacttt gacccacgct tccgtgcctt cttcaatttt
    23461 atccaaatct ccttccattt ctgctgtgaa atggatatcg acgatttgag ggaagaattc
    23521 acagattaat gaattgacaa tttctcctag ttcagtcggc tcaaatcttt tttgtgtcaa
    23581 ttttacatag tagcgtcttt ggatcgtatc aagtgttggt gcataagtgg aaggtcgccc
    23641 taccccgttt tcttctagtg cacgaatcaa cgtagcctca ctaaaacgtg ctggtggttg
    23701 agtaaaatgt tgttttggtt caatatcaac agattggact acatcgcctt cttcaagttc
    23761 tggaagaata ttttctttct cttctttgcc gtcatctcta ccttccacat acacttgcat
    23821 gaatcctttg aatttgattt ttgaaccgtt cgcaatgaag atgacaccat tttgttctaa
    23881 tgtcactttc attgtatcta aaatagctgg tgtcatctga ctagccacaa aacgtgacca
    23941 gatcaatgta taaagtttca attgatcttt atctaaatat tgtttgagtt catctggtgt
    24001 acgaagcaca ctcgttggac gcacagcttc atgggcatct tgtgcgcctt gagcattttt
    24061 gatctttcgg tgatcatgtc tagaaaattc ttttccatac gtatcttcaa taaatgctgc
    24121 ggcctctgct tttgctgtat cggcaatccg ggtagaatca gtacgcatgt atgtaatcaa
    24181 gccgactgtt ccttgtttac ccaaagtgat tccttcatat aattgctgtg ctaccatcat
    24241 ggtttttctt gttcgaaagt ttaacttacg ggcagcttct tgttgcaggc tacttgtagt
    24301 gaaaggattc gcagggtttc ttttgcgctc tttcttttct acttttttaa cttcgaagtc
    24361 tgttccatca attctagcag tgacttcttt tacactttgt gcatctggta atttcttttt
    24421 cttaccgtcg attccccaga aatttgcttt gaatttcttg cgagcttttt taaaattacc
    24481 gtcgatgctc caatattctt ctggaacaaa ttctcggatc tctttttcac ggtcaataat
    24541 aatttttaaa gcgacagatt ggacacgtcc tgcacttaac cctttcttga ccttacgcca
    24601 gagaatagga ctgatcgaat aaccaacgat tctgtctaag gtacgacgag cttgctgtgc
    24661 atctactaaa tctacatcga tcgttcttgg ttccttaaaa gctgctttga ctgcgtcttt
    24721 tgtgatttca ttgaagacca cccgattttt ttctttcaga tccaacccaa gtaggtacgc
    24781 taaatgccaa gcaatcgctt ctccttctct atccgggtca cttgccaagt aaactttttg
    24841 agcttttttt gctgcggctt tcaggctttt gatgacatcg ccttttccgc gtatagaaat
    24901 ataatggggt tcataattgt tttcaaaatc gatacccatt ttacttttcg gtaaatctct
    24961 tatatggcca acactggcca ttactttata gtttcgtcca agatatttct caattgtctt
    25021 ggcttttgca ggtgattcta caatcactaa atatttatac gccattactg aatggactcc
    25081 tttccattgt cgtgttccta ctattaaaag aggattcata caaatcgtag ctaatcatat
    25141 ccattcagaa aacgtttgtc aagaaaagca aatgaaaagt atggtgttat tctttaaatt
    25201 ctgccaagat atcttcaccc gaaaccgtgc aaactgcacc gtcttggatc aaatgatgac
    25261 atccggtaga atgttctgtt aaaatatttc ctggaacaga aaaaactgtt cttccaagat
    25321 ccaatgcttg ctgtgctgtt atcagagagc cgcttttttt cttggcttca aagacacatg
    25381 ttcctttaga caaagctgcg ataatccgat ttctcattgg aaaacaatgc tttttcgggc
    25441 cggattgagg aggatattca ctcaaaagaa gatgatcatt tgcaatcacc tcttgcaagt
    25501 cacgattttc agcaggatag acttgctcta tccctgttcc aatgactgcg attgtccgtc
    25561 caccatgaga gattgctgct tggtgaccgt atgtatctat ccctttcgcg cagccactga
    25621 cgatcacata tccagcttct atcactggtt ttagtaaata ataaactgtt tttttggcta
    25681 aagcagaagt gtgtcttcca ccaacaacgc ttaacatgtt acaagaaagt aaagatagat
    25741 caccattata aaacagtaca attggcggat aggtcaagtg aagcagctga gggggatatt
    25801 tgggatcaaa gcaagtgata aacgactgtt tcgattggat tttgccccag ttagcgtgaa
    25861 tttcctgcca acttttgcga aacagctccc ggtgtttggt tattttacct atcttaatga
    25921 tgtcttcaaa tccgatttct ttataaggat actgcatcat ccagttagca agtgcccatt
    25981 tcccttgact actgattcct tttgtataag caagtttcaa taaattttct tctatttgat
    26041 acatcataaa accgcctttc gtctctcttt ctaataagat acgtaaaagc ggctgttttt
    26101 tattgtaaat atttttttac aggtgcaaaa cttttacgat ggattggcgt aattccttgt
    26161 cgttcaagtc cttctaagtg ttcctttgtt ccataacctg cattatgcga gaaaccatat
    26221 ccaggataaa gcacgtcata ttctttcatc aattggtctc gatatacttt agcaatgata
    26281 cttgcagcag caatcgacac agatcgtgca tcccctttga ttattttttc ctgctgaata
    26341 ggcaaatcca aggtcatcgc atcgatcaac aaatagcttg gtacaatgga caatttttct
    26401 actgcttgga tcatcgctaa tttacttgct tcataaatat tcacatcatc gattgtctgc
    26461 tgatcaacga tcccgatccc gattgccagg gctttttctt gaacttgttg ataaagaagt
    26521 tctcgctttt tctctgataa ttgtttggag tcattcagcc ctaatatgat ctcatttttc
    26581 ggcaagataa ctgcagctgc cacgacaggt ccagctaatg gtcctcttcc tacttcatca
    26641 atgccggcaa tccattggtg accttgtgcg tacccttgct tttcaaagca aaacatttct
    26701 tcatattttt ccttaattaa tgcttccttt ttcaatcgtc gttcaaaaga agccagtgct
    26761 tgctgcacgc ctttgcgtgg atcatttctt aatgattcta gtgtaggatc atctattctc
    26821 tgtatgcttt tcaatcgttc tttgatactt tggatggatt cagtcatcac ttttttgctc
    26881 catttcttcg caacgatcca gtgtgtatcg tccaagtttt ccttgacgaa tatcatgcac
    26941 gactaattcg ctgccacgat catagtcttc tttataccca cgttttgctg taatatccat
    27001 cagtaattcc ggtgccaatt tttcttcttg ctcgattgat aaaccataac gatcgctgat
    27061 tcttcctgaa taatatcggg caaaaaagtc taatccgtaa atcgctaaat catctaaatg
    27121 gacaagctgg tctttgatcg cgcctgtcag cgctagtttt ttgccgattt cttcatcttc
    27181 aaatttgggc cataaaatcc ctggtgtgtc taacaattcc aactcattac ctaaacgaag
    27241 ccactgctga ccttttgtta cgccaggctt atttcctgtc tgtgcaattt tcttgcctac
    27301 tagtcgattc atcaacgtag atttcccaac atttggaata cctaagacca ttgcccgaat
    27361 agcgcgaggc ttgatccccc gagcagcatc tcttgcccgt ttttctttca atgcttcttt
    27421 agccgcaggt actagacctt taattccttt actttcttgt gccgtgatag ctaatgcaag
    27481 atggccttgt ttacgaaaat attcttgcca cagctttgtc tgttgtggat ctgccagatc
    27541 cgctttattc aataaaacaa ggcgaggttt ttgctgaatg atctgatcta gcattggatt
    27601 acgagaagat aaaggtaatc gtgcgtctac caattcaaat acgatgtcca catatttcat
    27661 tttttcagaa acttctcgtc gggcttttgc catatgtccc ggaaaccatt ggatggtcat
    27721 tgttctctcc attcctttca taccggaata ttccccggct ttacttgttt ttttcttttt
    27781 caatataaat tcactttttt attataaaga aattccatct attagttaag tgaaagtgaa
    27841 ccagtcctca ttctatcaca tcttcacttt tttgatgatg caaattttta ttttggtttc
    27901 tatatatgaa tctaaatttt tgacagcgtt tcctaaataa gagatgatag acgaacaaaa
    27961 cagaaaggag acgaaacaaa tgaatagtat ggtttttgtt aattttcctg ttgctgatgt
    28021 caatcgttcc gttgcttttt atgaaaagct gggttttaaa aaaaatgaag aattttctac
    28081 tgaagaagct ggttcaatgg tatgggatga cactttttgg ataatgcttc tctctcatga
    28141 tttttataaa aaatttataa aagataaaga aatagctgat aataaaaaaa caagtggtgc
    28201 tttgatttcg tttagtgttg aaaatgcgga tgaagtaaaa aaatttgctc aaattgcaaa
    28261 agaaaatggt ggtactttcc acgaagtaga tatgggaatg cctgaagaag aaatgttttc
    28321 attagaagtc caagatctag acggaaatac attagaaccc gtatggatga agatgtaagc
    28381 agataactaa tctgacgttt tttataacaa aaaaacaaag gcggatgata cgataagtat
    28441 tctccgcctc tatttttttt attcgtacaa tctctttttt acagtttata aaagcagtct
    28501 tctcaccctt tttttaattg ttagatgcca gtgtatgtaa atcaccaatg tgtcatgaca
    28561 cattggtgat tttatggtat ccttttatta ttaactgaca aaggagagaa tattttgaac
    28621 actttgattg caaaagacgt ttttagattg cccgaaatga atgcaagatt gttgtcgacc
    28681 tctgctcttt tattagccct tgatgtcatc ttatacaaga tagcgattgg tcctgcttat
    28741 tttcaaatca cattcggatt tttaagtatg gcgcttatgg gttatctata tggaccgatc
    28801 tgggctggtt tgcttgaaat gttgtcgaac actttaaatt tcacgatatt tggttcagga
    28861 acgtttcaag ctgcttttct tatcacggct ttccttggtg gatttattaa tggcttattt
    28921 ttatatcata aaaagataaa cctgccttcc gtcatcgctg cccaattatt gattatgatc
    28981 ccagtcagcc tttttatcaa ttcttggctg atgtctgttt tgtttggtgc agatttcaaa
    29041 gtaatttttc taggtagatt catcaggaat atgatccaaa tccctattca aataatggtt
    29101 gtttattcat ttatcaaaac tttagaggct agaaaagtgt ttaaacgagc tgtttgattt
    29161 tacgtactaa ggtagtaaac tgattttgga ttaaacaata aagcgtgcag tctcaacaga
    29221 ctgcacgctt tattgtttct cactggatcc attttatttc atcaagtgga taatagacaa
    29281 atcttgcttt acctagaata tcagttttat tgatcgtgcc aaacgaacga ctatcttttg
    29341 aaactcttcg attatctcca agtacaaaat aattatcttc cggaagcttg gattgtcccg
    29401 ttagctcttg tagtgtaaag tccgtcgtat aggatgcatt tgcatgatct gatttcaggt
    29461 ttttagttag ataaggttcc ttaactactt tcccattgat cttcaattga tcattttggt
    29521 agctgacatt ttctcctgga agaccgatta ctcgtttgat ataaatcgtg ccatctgcta
    29581 attgaaaaac aacaatatca aatcgtctga tctccgaaaa tttttccatc acaaccatat
    29641 caccttgctt caacacgttt tccattgaat tgccttccac tggtaccggg atgaataaaa
    29701 atccccgcaa catgaacgca aggaatacag aaacaagtaa gtatttgaat acaagccaga
    29761 agcgattaat atagcgttgt ttttttgtca atctctatca atccatccct ctttttttca
    29821 ttctttcttt attataagaa aaatatcaaa aaaatgcgac tagatagctg aaataaaaat
    29881 aattctttca attaggctat tggacaaaaa acttttttga tcataaagta caaaactatc
    29941 tcttatatgt catgattatt ttctaaatat ttttcccatg cttcgtcaaa gatatccatg
    30001 gacggcaaat agtcgacatt cagttccaga taattagaaa tttcttcgta gctagagctg
    30061 tgcttaggaa attggatgtc tttaccggct tctgtagcga aaacagataa agcatcatgt
    30121 gtgccaccgc ctcgcagtgt cattaaataa tgaaaaaagc ttcgattcac ccttcgttcc
    30181 tccaattttc ttcgataaag gcagcccggc gtgcatggtt tcttttaaaa ttttcaggat
    30241 gctttttata atactcttga tggtcttctt ctgctagata aaatggttgg gctggttcga
    30301 tttttgtaac gattggctgt tcgaaacgtc cactttcagc taactgtttc ttactttttt
    30361 cagcaatgat ccgctgttct tctgtcaaat aaaagatcac cgggcgataa ttgtcaccgc
    30421 gatcctcgaa ttgtccgaaa gcgtcagttg gatcagtttg ctgccagtaa agttctacta
    30481 attgttcata tgaaatcaat gttggatcat agataatctc cactgcttct gtatgtccag
    30541 tcgtttggct ttttacttgt tcataagtcg gatctggtac atgccctcca gtatatcctg
    30601 acatcactgt atggatacca ggaagcgtgt cgaaaggctg gatcatacac cagaagcagc
    30661 ctcctgcaaa tattgctcgt tccattatga ttccccctct aatactactc atccgatgat
    30721 tttttatttt cttttgtctt gctttcagta ctgctggtgc tagttttgtt ggtatttgag
    30781 ttgtttgaag catcagaggt atcttttggc aaatatagac taaaccgaat gtcatcattt
    30841 actaagtcga ttttatctgc tttaatatac atcccgtttt gcattcggaa ttgatccaac
    30901 ctaagcatga tagtcagatc atctgtattg acttcgatcc aatctggtaa tttatagttt
    30961 ctcttgatca tattcatcac atctttgatt ggcaaactca atgttccgat agaaagactt
    31021 ctagctttta gttggacatt tccgttttcc atcacatacg gatcaaaata taaatagaaa
    31081 tcgataggaa aacctagtac ttcgaaagta ccattgagta gtgcttcatt ttccaaataa
    31141 aatttatatt caatatcact atctttttga tactcactaa ggaaaaagct gatgatttga
    31201 ttgacttttt ctttatttga attgatcgtt aagacaggat ctccttgtct ttccgtaaga
    31261 gctggtattt ctgggagttc tggttcccga ttagcaaaaa tacggctgcc taaaaatacg
    31321 gtcgaaccaa gtactacccc aagaaggata aggaaggcga atttccagta atttgttttc
    31381 tttgttttgt ttgaagatac gtcagatctt gttttcatgt ttattgatca tcctccttct
    31441 ctaaccattg gttttgtgtc tcgatcagct gatctctcac tgcatttgcc atcaattgat
    31501 aacctaaatt atttggatgg aaatgatctt cttcatagag cacattattt ttgatgccat
    31561 ttagattttc tgtcgtgcta gttgtttgat ctgttccttc ttctgtgaca cctacttcct
    31621 gattcaatcc ttgataaagc aaatcattga ttgggataaa gtagcagttc ggcatttttt
    31681 ttgtcaccgt ttctgttcct ttgttccaat tgttaacaat tgtctgcatg tcagtgatct
    31741 caggaaagtt caaataaaat ggattgtaga tgcctaaaat gtaaataggt gcggttttat
    31801 tgagggatcg aaattcttcc aatagttctg tgacattttc ctgatagttt ttcagtggct
    31861 ttttaaagga attgaccgtt aacccgaata aattattttg gataactttc atcaaatcat
    31921 taccgccaac agtcagtgtg ataaaatcag ctgttttgat attgtttcga atttcttctt
    31981 ttgtttttac tcgctttaag atctggtcac ttcgttcacc atttacacca tagttttctg
    32041 tttcaaccgc ggtcaaatta taacggtctt ttagatcatt tgcgacaagg ggaacgaatc
    32101 ctccttgctt ggtttgatct cctactcctt cagtcagcga atctccgata gctatgtagt
    32161 gaagtttttc ttttttattt ggatcttctc tcacagtagt tactggtttc attagtgggc
    32221 cagctttcgg tatagaagca gctaaacctg catagatgat gatcgcaatt aataatggga
    32281 acaaaacgag gatcaaatgt cgtttcgtta atttcatttt ttcacctctt gtattcgatt
    32341 ggttctaatg ttatcaaaaa ttttatcaaa aaggtagtct ttatgaaaaa agaaaacaat
    32401 tattttccta attttataaa gaaaaagaga ataagctgca agcctttcag gctgagggct
    32461 tatcctcttt tcgactaatc tacataatac atgattgcaa acgcgtcttt accagtatgc
    32521 gtagcgataa ccggattcgt atgcagtaca gggatatgca tgtccgggaa taattcttgc
    32581 aaaccctctt tgaattcttg cgccaattct tttgcatccg catgtgaaat accgatttgt
    32641 cgcacatttg gcaattcttt cagttcattt ttcaaattat taaaccattt atcgaacgtt
    32701 ttctttcctc gccccttagt tacagggatt aattcagaat gggcgaaatt catcaccacg
    32761 cgtatgttca aaacactaga cagcaaacca gttgttcggc tgattcttcc accttttacc
    32821 aagttatcta gtgtggatat tccgatgtat aattttgtat tgtcttgtac ttttttgact
    32881 tcttcaagga ttttctctac tgttttacct tcttttgcta attcagctgc tcgaattacc
    32941 tgaaaagata atccttggtc agtggtatca ctgtcgataa ccgttacttt cgtttttgtt
    33001 agattactgg cttgtcttgc tgcttctact gttccactca agccttttgt catatggatc
    33061 gaaatcactt cactgccgtc ttctcctagc tcatcataca tcgagatgaa ttctccaatt
    33121 ggtggttggc ttgttttggg cagatttttt gctttcgcca tcatttccat aaatctttcg
    33181 ccgtctagtt ggtcatcatc gggatatact gttccgtcga tcatgatgga tagcggcatg
    33241 acacgaatgc ctaaagcatc gcgtacattt ttttccatcg tacatgaaga atctgttacg
    33301 atttttacat ttgtcattat tttctctctc tctttcttta acaagatgcc ttattcatgt
    33361 taaactacat gtaagacaat ttctattttt agtataacag acaaataatg atttttcatt
    33421 aattacacct agtaaaggag ccgttttcat ggaaaaaacg aaatttaatc gaacgtatga
    33481 aatattaaat gaagtgttta atgcagtcac ccatggaatt gggactggat tgagtatcgc
    33541 gggactagtg gttcttttag taaaaggtgc ccacttgcat tcctccctcc atattgtctc
    33601 ttatgctatc tatggatcga tgatgattct tttattttta acgtctactt tgtttcattc
    33661 attgattttt acaaaagcaa aaaaggtgtt ccaagtattc gatcatagtt cgatttttct
    33721 tttgattgca ggaagttata ctcctttttg tttgctgagt atccgaggat ggctaggttg
    33781 gactttattt gtattaatct ggttgtttgc gattcttggc attatatata aagcgttgac
    33841 gttgcataaa cgggagagag tatcaaaagt atctacgatc atctatattt ttatggggtg
    33901 gctctgtgtc gtggctgctg ttcctcttta tcactctctt ggaatgactg gtgttatttt
    33961 gatggtagca ggcgggcttt cttacacgat aggcgcattc ttttatagct tgaattctat
    34021 ccgctatatg catgttgttt ggcatttgtt tgttatgtta ggtgcaggtt ttatgtactt
    34081 ctctgtttta ttctttactt aataaaaaaa ataaagctat ggatgggaca gctctaaata
    34141 acagctagtc tatccatagc ttttttatat cgtcaattcg cctgtcccgc tttttacagg
    34201 attctcatta cgctgatacg tttcaaattg atgcggataa cgattttttt catccatttc
    34261 tccttgacta gtattgatca gtgaccaatc agaccagtca acaggtggaa aataagcatc
    34321 tccatcgaat acatgatgga tgactgtacg gtataatatc gtacaaaatg gcagaaattc
    34381 ttgataaaca gctgaccctc cagcgatgaa aaagataccg tctgtttctt tttccagagc
    34441 aagcacgtct tccacactat gcatgacatg cacgccttct gctttatagg aagaatctct
    34501 cgtcaaaaca atcgtctgac gattcggcaa cggacggcta cccatccctt cgaatgtttt
    34561 tcgtcccatc actagtgtat ttgcttctgt catttgtttg aagaatttca gatcatttgg
    34621 caaacgccaa ggaagacggt cttcttttcc aatcaatccg ttttcatcct gtgcccaaat
    34681 tgcagctaac attttctcat cctttctgat tctaaacagc gataggagct ttgatcgccg
    34741 gatgtgggtc atatccttct aattggatat cttccatctc aaaatcaaat actgactgct
    34801 tttcctgatt caaaactaaa gtcggaaatg cgcgtatttc tcggctcaat tgttctttca
    34861 tttgttcgat atgattgata taaagatgag catctcctaa tgtgtgaaca aattcgccaa
    34921 ccgctaaacc tgtttcatgt gcaattaggt gagtcagcaa cgcataactt gcaatgttga
    34981 acggaacgcc tagaaacaca tccccactgc gttgatacaa ttggcagctt agccgtccct
    35041 cttgaacata aaattgaaac atcgtatgac aaggaggcaa agccatgcta ggaacatctt
    35101 ccggattcca tgcagaaacg atcaatcttc tagaatcagg agtctttttg attgcttcga
    35161 tcacttgttt gatttgatcg ataaagccac cgtcttttgt ttcccagtga cgccattgat
    35221 agccgtagat gtgtccaaga tcgccgtatg tttcagcaaa ggaatcatcg gttaggatct
    35281 tctcgcaaaa aagcttgctc tgctttttat attcttcatt gaattcagga tctgtcaaac
    35341 atctatggcc aaaatcagtc atatctggtc cattatagtc tggacttttg atgtagcgtt
    35401 caaatgccca ttcatcccaa atatggtttt tattttgtaa tagatagcgg atattcgtat
    35461 ctccttttaa aaaccaaagc aattcgcttt tgatcaatcc aaaaggtact cgtttcgttg
    35521 ttaacaaagg aaaccctttt tgcagatcaa accgcatttg gtatccgaat aagcttaatg
    35581 tgcctgtgcc tgttctgtct tctttacgat gcccttcttc caacaatttt cttcctaagt
    35641 caagatacgc ttgttccatc ctttttcctc ctaatcttct acccattcac tcaagtattc
    35701 ccaccgtgcc attttttctt caagttgtgc ttctgtttca gatacgtcgt tttgcaattc
    35761 ttgtaatcga gtgaaattat ctccttgatg attcatctct tcttggagca atgagatttt
    35821 ttcttcaagt tctgcaatct catcttcaat cgtttcccat tctttttgtt ccatgtaaga
    35881 taatttcttc ttttcagcag gttccttgct ctgtattttt ggtttcgctt tttcagcagg
    35941 ctgctgggtc tctttttgct tcagtaaata actggacatg tttccgtaat aaagttcttg
    36001 ttttccgttt ccttggaata ctaataactt atctgctacc ttgtccaaga aataacggtc
    36061 atgggaaaca gtgatcacag cacctggaaa acttctaaaa taatcttcca aaatcgtcaa
    36121 tgttgctatg tccaaatcat tggtcggttc atccaataat aaaacattag gttgctgaat
    36181 cagcagtttc aacaaaaata accggcgttt ttctccgcct gaaagtttgc ctatcagtgt
    36241 cccatgcata aatcttggaa ataaaaatcg ttcaagtaat tctgctactc cgatttgact
    36301 gccatctgct gtttttgctt cttcagcagc ttcttgtaag taagcaatca tccgtttatc
    36361 tggtgccatt tcttcatttt cttgtgtata atacgcaaga tgaaccgttt caccgataga
    36421 cattgtaccg ctatccaggg gaattctgcc agctagaata ttaagcaatg tcgattttcc
    36481 cgcaccattt ttaccagtaa tacctagtct ttcccgagat tgaatcaata aatctaaatg
    36541 ttctagcagt gtttggtcat ttatacggta actgccatct ttgatttcca gtactttttt
    36601 ccctaaacgt tgtgtcgcta ggttcagctc taaatcatct tcttggttta cttggtacaa
    36661 gttctccttt agatcttcga aacggttgat ccgtgcttgc tgcttggtgc cacgtgcctg
    36721 tacgccagcc ctcatccaag ctaattcctg tttataaagc cgcttgcgct tttcttcttg
    36781 ttctacttct acacgatcac gttctgcttt ttctaagacg taagcttcat aatttccttt
    36841 atactcgtat aattttccaa atgaaagttc gaagattcgg tttgctactc tatctagaaa
    36901 ataacgatcg tgggtaacca tcaatagtgc gccacggtat tgtttcaaat agttttccaa
    36961 ccactcgatc gcttcataat ctaaatggtt tgttggttcg tctagtaata gtaaatcggg
    37021 ttcatcaatc aacacttgcg ccagactaac tcgttttttc tgaccacctg ataaagtact
    37081 gattttttta tctaattcac tgattcctaa tttttgcaaa ataatctttg cgttggtatc
    37141 tgtagtccaa gcatcttctt tattcatttg ttcttctgct ttagcataac gtttttgcac
    37201 atcagggtct tctccgtttt cccctagttc gatcaatgct tcctcatatg ctcgaacagt
    37261 ttggatcaag gggctgtttc cttgaaagac tgcttggaga acagttaatt catcggaaaa
    37321 ttcagctgct tgagaaagat aaccaatacg gtaatcagtt ggataaaaca ctgtttgccg
    37381 atcaccgtcg ctgctatcga taccggcaat aatatttaag agacttgttt tacccgtccc
    37441 gttcgtccca atcaacccga tacggtcttt ttcgtggacc aaaaaagaaa tctggtcaaa
    37501 caaatctttt tcaccataag tttttttgag gtcagttact tttaattctt tcaacatgtt
    37561 ccctccttat cttggatatt gtatcataaa aatagtgaac ttcaggaaaa tttttttggt
    37621 atttgttctt tttgtgttct caaaaaaaga acaggaccga ttcgatcctg ttctgatatg
    37681 ttttgttttt tataaaactt gaaaattttt acctttttat tttattggac tcgttttttt
    37741 tgactttgct cgaaacgcaa atcggaaaga aaagttatca gatggtttgt tcatcgtata
    37801 agattctaac aaattgatat tcctgcgata gctgttcgca gaaaacgtcg tgattttttc
    37861 agcaacttcg tattcatcaa tgatttttct ttcaagataa taacctctta gcattttttt
    37921 cgtaaacaga ctatcttgct ggatcatcca cgaagaaatc aaattccccg agttcatttt
    37981 tattccttca agtttgcgtt gctggataaa aaattgaacc atcgtttcat cataaatatc
    38041 agtcaagtta tctaaacttt caataatcaa ctgagtattt ttttgatatg tctctctgat
    38101 ttcttggata tcttcatcac taataaatgt ctgacgatca gcatatcttc tttgccaaaa
    38161 cattttcgga tggagaacga ttcgcattaa cctacggaca acaatgaacc aaaagccgat
    38221 aaaagacaaa atctggcttg tgatagaatg ttcgaatcga gaaagaaaac gggagtaaaa
    38281 acgataacca ttagaagaaa gcttgcccga acgataactt tcatccaatc catcccgttc
    38341 gatcgacaaa atcagtgctt gtatttcttg tacttcttgc ttttcatcat cggttaaatc
    38401 ttccaagtac agatcacgta tccgttcttg ataattttct atcactgctt cagtggctaa
    38461 aaattctttt gtattacgat caatttcctt tatttctttt cgcaaaactt caacgacctc
    38521 ttctaaaatc atcaaagcat tcaaatccgt gcttttcgct tcctctgttt ccgtcagcat
    38581 aggcaagaca atgatcccta tgaccaaagt cactaagatg acacccgccg tcagaaacaa
    38641 aagcaaagac cgctggtaaa acatcatatt attgattgaa aatggcaaaa taaaaatcgt
    38701 agccaaactg actgttccct ttacaccgcc aaatgttaag atcaagatct cgttcaactg
    38761 ttttttgaac tttttactgc catctttgaa tacataaaaa agactgatag aaatgaatct
    38821 gatcacaaaa agcatgacag agattaatac gataatcgct aataaaagac cgtttggata
    38881 taaaccgtct ccccatactg gcgagaacac ttgcgtcaat tcgattccta aaaatataaa
    38941 tactaatgca ttcagtgtaa aagcaatagt tgtccatgta ctgtgggaca agctcgacaa
    39001 ttctgcatca aacacagaga ttttcctaaa accagaagct tggagaatac ctgctgccac
    39061 tgcagcaatg attccagata catcaaatgc ctctgccaat acataggcta gaaaaggaag
    39121 caaaagttcg atcagcagat accctgtgac atcttgtgct gatgcttttt cgatcaaatg
    39181 aattattttt tgtttgaacc agacaagcaa aaagccgatc aaagctcccc caatactaga
    39241 aactactaga ctcatccccg cgtttactgc tgaaaaagat ccggtgatca gcgcaccaag
    39301 agcaaactgg aaggcagtaa caccagatgc atcattcaaa agaccttctc cttccaaaat
    39361 atgcatcgcc tttttaggga tattcactcg tccagaaaga gaactgactg ctaccgcatc
    39421 tgtcggacct aacgctgctc caaaagcaaa gcaggcagca agtgggacac ttggcaacag
    39481 gaatgataac gtcgcaccta ccccgacaag ggtcaaaatg actcctccaa atgccaagaa
    39541 caaaattgtc ccgaaatttt tgagaatcga agaaatatct gctttttctc cttctctaaa
    39601 taacaatggt gcaataatca taactaagaa caattcaggt tcaaaatcga ttgattgtcc
    39661 ccactctgtc agacctaata ccactccaag gaaaatttgg atcagtggag ctggaatcat
    39721 tggcaaaatg cggctagcta tgttcgataa ggtgatcgtt actgcaaata caataactaa
    39781 ctcagcaaat tccatcgtcc atccccttta ttttttattt tgtgtaagca tttttaaaaa
    39841 ttgttctttt ttctttaaaa ttttggtttc gactacttca acctgtgttt ggttcccctc
    39901 taattcacac tgtacacgtt ttgactccaa acgaagcaga tcatgtttca gacgttccaa
    39961 ctgacgatac atcaatatat ttcttttacg caacttacaa agtatttgac ggcggtcatc
    40021 catcgaaagg gcgccatagc gatcatgcaa cagttttttt tgttgttcaa aactctctct
    40081 cattttccag atctcctata cacaaacttc catcattata gcaaagttct ttttggatga
    40141 aaaatatttc attgtttcat cgtttattgc tagttagaaa tttccataaa tttttcaacg
    40201 cctcattcta ttccaaatag tatcagatat caagtaaaac ttaagaaaga taaaagtttc
    40261 tgacaaaagg atgtttactt tcccattcct gcctttcttg ttcttttatc acaacaaaaa
    40321 aatggggctg cgacaaaaat cttgtcacag tcccttattc tgaataaacg gtgtggaaga
    40381 gacagcctat cagtagtaat aagacacatc tctttcacag cctcattttt gcgtaaaaat
    40441 cgatttattc gaaaaattcc ccgtatacat cttctacacg ttgatctgga ttgatcacga
    40501 tatacaagtt acctgttttt tcgctgattt ttgttgattg atattgattg tctaatcctt
    40561 ctgttccttt atctagatat gggaagtaca caagatcagg tgtgccattt cggtaaagga
    40621 tttccattcg ctcgatatct tcgtatttca atgcgcgatt aaacatgccc atttccagtc
    40681 cgcctaggtt gatatcatgt gtcgcaactg cgtccccttc atggaagatt tcgattttaa
    40741 atccagcaca cggatggatt tcaacaaatt cactgccatt gattcgtcca aaacttgttg
    40801 tgatttgttt gatccaaaga tcgccgatat gacggcggtt gatcgtccag gtttctccat
    40861 ttctaaaata aaattttagc gctgtcattt ctttactcat taaaaagact cctttatgat
    40921 gtaatttatt cttgtactac ctcagtttag cacaaattta tgtaagtggt cacatcttat
    40981 gttttttctt ctaatgacgt atctgcccac tttaagtagc caacaaagct gagcagatac
    41041 agtctagtgt tttttaaatc aatttttctt tcgataaaaa atccaaaacc tgttttttat
    41101 cattttttat tctttgttct acgacggctt tttcagctaa ttgcaaagct tcactaaccc
    41161 acttaccagg tttttttcca gtttcttcta gcaagatttt accatctact gctaattcgc
    41221 ttaatgactt gataggtaaa cgatcatagg attctcctac ggcatccatc tgaggtgttt
    41281 cgtcaaaata gatcatcaat tcttctactt gcagtgcatg ttttttgcct aagaagtaca
    41341 attcgaatgg ttccaaagct ccagtttctc gttgtcgtag tccttggaca agtgcttgaa
    41401 cgtctttaat catttggtta gaacacttcc acgatttcaa aaaggaacga atctcatttt
    41461 cttttagatc cagttggctg atcaataatg cccatgcttg gctctcttct ttcaatggga
    41521 cggatggtaa ttcagcaaaa cgtaaaagtg cttcgccttc ttctttcaaa ccgggacagc
    41581 aaatgtagca ctctgtttca ataaatgcat ggattcccgc ggcccgttca tttcccaaga
    41641 gcatcttgat gaattccaca tttatacgtt ctacagatat tttacctaat agctcgtggt
    41701 tttctttgat ggcgtagaag gtttcttcct ctaatttgaa ccctagttga ctgacaaaac
    41761 gcaacccgcg catcattcgt aaggcatctt catggaaccg ctcatgagga ttgcctacag
    41821 ctcgcaagat ctgttcttct agatcttgca gaccatcgaa caaatcgatg atttctccat
    41881 cttctttcaa agcaaacgca ttgatcgtaa aatctcttct tttcaaatct tctgccaacg
    41941 aacgaacgaa ttcgacatga tctggtctac ggtagtcctg ataagttgat tccgtccgaa
    42001 atgtggtgat ctcatattgt tcatcatttt ctaataccaa cacagttcca tgttcgatcc
    42061 caatatctat cgtttttgga aaaagctgtt tgatttcttc tggaaatgcg cttgttgcga
    42121 tatccacatc atggattggc tggttcagca agatatcacg aacacttcca cctacaaaat
    42181 aagcttcata tcctgcattc tctaattttt tcagaactgg aattgccttt tggtattcca
    42241 gaggtaattg ttttaatttc atttttccaa cctttcctgc gtgtttcttg tcctgagata
    42301 aagactcagt tgcatggtga ctgtttgaca agtccgtttc agagtcttca aggccaggac
    42361 cacttcatct tgtttactcg tccttacgct caaagcgatt atggtctctt tgattgaatt
    42421 tttccatgtt tttttcaaaa acttcagtta agtcgatatt caatgaatta gccatgatca
    42481 tcgtaacgaa taaaacatca ccaagttctt ctgctaccga gttaactggt tcagaagcct
    42541 ttttgctttt ttctccataa gtgtggttga cttctcttgc caattcacct acttcttcag
    42601 ttaatcgagc catctgagct aatggagaga aataccccac cttgaattgc tggatatatt
    42661 catccacttc ctgctgcata ctgtataaag aacgcttttt atccatcttt ttccctccaa
    42721 aactatctta ccaaaacttt aacgcaaatc ctagataaga gcaattgaga aaatcgaatt
    42781 tccatgctac atttaggaag gaatttctaa atggaaattc tgtgaaaata cataattgaa
    42841 agctggaatt catatggtca aacgaaaaac agtaaaagaa gttcttttta tcattatagg
    42901 aacaagtatt tatgcatttg gtcttgttta cttgaacatc gctaatcatt tagcagaagg
    42961 gggagtctca gggatcacct tgatcttaag agctttattt ggtattgatc cagcttatac
    43021 gactttatta atcaacatcc cattgatttt actaggagga aagatcctcg gtaaacgttc
    43081 cttggcctat actgttctag gtacactttc tttgtcattc tttttatgga tctggcaaag
    43141 ggttccacta gaaatcaatt tacagcatga tctactgatc gtttctctac ttgcgggttt
    43201 agttgcaggt gtaggaagcg ggatcgtcta ccgcatgggt ggaacaacag gcggcagtga
    43261 tattatcgct cggattttag aacaaaatta cggcatcact atggggcgtt ccctattggc
    43321 atttgatatc atcgtcttac ttgcttcatt aacctatatc gacttgaaac gtatgatgta
    43381 tacgttgatt gtttcgtatg ttttcagtcg tgtgattgat tcgatcttag atggcggata
    43441 ttctgcaaaa ggtctacttg tcgtctccaa caaaagcgaa gagattgcac cactactgat
    43501 gaccggtctg caaagaggtg tgactttcct ttctggagaa ggcgcattct ctggtaattc
    43561 aaaaaaaatc atctatatgg tcgttagctc tagtgaatta actgaagcta agaggatcgt
    43621 ccacgagatt gacgaaaaag ctttcctttc aatcatcaat gtgcatgaag tggaagggca
    43681 aggctttact tatttgaagc cacagacaag attactgaaa aaactataag aaaaaagaca
    43741 aagataggct atgaggaaag attccacatc cagctgacct ttgtcttttt tagtactata
    43801 ctatttttaa ctaattgtta tgttcactta cccggttact tttcttttgt gtctgcaaaa
    43861 ggatctttcg gttaacttca tcagataatt tcaaaaaagc atccctatct ccatcttcta
    43921 gtgcttgatc gatttgagca taaagcattt ttagttttgc ctcttcttct ttttcttgaa
    43981 aatatcgttc aattaattca gtatcttctt cactgactga agaatcccaa gaagaaaatg
    44041 gattgtcttc taagatagat agatattctc ttgtctgcca agcattttcg aatatacatt
    44101 ccacatataa aggctttttc caattcaaac ggatttcatg aaaaatctgg tctgtgtccg
    44161 taaattcttt tccctgcaaa aacaatttca tgggttcccc ttcaacagat agatcactga
    44221 tctgcagtcc tcgatccgca aaattcgccc cttcaacaaa gtggacattg cttaaaatcg
    44281 cttcatgatt ggcaagataa ttcaagatcc aaaaaacttc tcttcggcta aaagatacat
    44341 gattgaccat ccacgttaaa aaattcttct tttcccgtac atcaattagc atttttctca
    44401 cctctctgtt aggtcatcta ataatgacag tgcctccata tctcctggtt caagtgttaa
    44461 ataataagtc aataattctt gcgctctttt cagctgccct tcttctctta agaaaaaggc
    44521 atactctttc aagaaatctg gttcatgtga tagttcatga taagcctgtt catagtgtac
    44581 agctgcaacg gtaaagtctt ctaattcatt ataagcatgg gctaggttcc attctgcata
    44641 aggattagca gtttcttcca tctgattgat cacttgtatc acatcttcat atctttcatc
    44701 atccaagtaa agattactta aagtaagtaa tgtttcatct tgtttgtctc ctacttctaa
    44761 agctttcagt aaaagttctt ctgctcgctg tctatcatgc agacggaaag cattttccga
    44821 ggccaggtga taaagttcga cttggtaagg attttctttg attccttctt ctaacactgt
    44881 ttgtgcttct tcaatcattt cttcttcttg aagagcttgt cctagatata gatataacga
    44941 ttgataatgc ggattaactt ctcgaagttc ttccagatag ttgattgcct gctgattttc
    45001 tttcaattga agattcgtaa aagctagctg gaacagtcga tcatctgtcc tttcttcttt
    45061 cagtgcttct tctaaaggta cgatagctgc ttcgaattct ccttgcatac ttaaggattg
    45121 tcctaaacgt tcctgcatcg agattcctga aatttcattt atcccgcttg ccaacaatgt
    45181 ttcataaacc actgctgcct ctttgaaacg atcaactgaa aaatacaatt cagctagcgc
    45241 aaactggatc aatggttcat caggaagcaa cccagctgct tctttcaatt ttgcttcact
    45301 cacttcaggg attccgataa cttggtacaa atcagctatc gccaataagc tttgcggata
    45361 gtaatcactt tgcttatcca ctttttccag gtattcaaaa gctaagtcga tctcattatc
    45421 ttcgattgca atttctgcta acgggatgtt cagtccgtca ttttctgggt attggttgat
    45481 cagttgttca aaaatctgtt ttgcttcgat caaaaagcca atagccagca attcttcgcc
    45541 tagttcagct aatatatctg ctgggtcttc ttttaatgct ttttctaaaa agatttgtgc
    45601 ttgtgacaag tcttcatctg ataaagcttg caacattttt tcgctatttg tattcatttc
    45661 aattccctct tatctcactg acgatttgtt gaaataccgt cattgttctc aataattctt
    45721 tttcgtttgg atgttggtat ccgtctatta tacctatttt ttctaaaaca agtatttttt
    45781 tgcttttttc tgccaaaaaa agaacttttt ccaaatactc catctttgaa atctgttcca
    45841 gccagtggat tggatagcca agcaagtcca gccacacaaa aaagtttttt agttggaaat
    45901 caaaaggata tgcttggaga ttccacacga aatgaaacat cattcctaaa aattttttca
    45961 tactagcaga aagtagatgg ccattttcgg tcagataaaa agcttgttca aacagtttac
    46021 cataatttga caggttttct gcctctaatt gataaaaata gatcatttct tcgatcatcc
    46081 cggcaaacgg ccgagtcgtt aattgctttt tatttggata gtttttatac agattttgca
    46141 aaaaacgatg atcacaaatc attccgcaac agataaaagt caaaaagtca atctgctttc
    46201 cgtccacctg ccgctcgctg attgtttgat cataaacgat gcgttctggc agattgatgg
    46261 tttgcagcac tacacgattt ggtagctgca aaatcgtccg ctgcggtgtt aatgctcggc
    46321 ttagagaacg gatcgataca gggatcatcc atagtgatga atcaagggtt gtatgttttt
    46381 gaaaaaaacc tgccagatcc atgatccctt catttccaaa tccgatgata aacatggatt
    46441 gattttcggg atagcgtttg acgaaatcaa gcaggttctt taattctgtt aaatgattgc
    46501 attgagtgtt ggcacaaata taccagtcat tgtttgcttg atttgcaaat aacgggttga
    46561 tcttatctgc aaacaaatca taatagcgct gattcgttaa aaataagatt ggctgatcaa
    46621 tggaaagttc tcttagctga ctgactagcg tttctccgta aaacactttc gtcaacgcat
    46681 cttctacttg ataatcaagt tccatacgca tccctcctct tcttattttt attttaccat
    46741 tagtaagcaa attctagaaa agataagagc aaagctcacc tcataacttg caaataaata
    46801 aaaattaaat aaaatacatg tgttttctat gtatgacata cggattcgga atctctgtct
    46861 cttatttatt ccttttctgc taggaaataa aaagaaaagc acttgtagcc ctagagccac
    46921 aagcgctttt tcaactgtct tatttaacag cgtcttttaa tgctttacct ggtttgaatg
    46981 caggtacttt gctagctgga atttcgattt cttcaccagt ttgtgggtta cgacctttgc
    47041 gagctgcgcg ttcgcgaact tcaaagttac caaaaccgat taattgaact ttttcgccgt
    47101 tagctaaagc atcttgaata gttgaaaata cagcatctac tgctgcagtt gcatcttttt
    47161 tagttaaatc agtagctgaa gcaacttttt cgattaattc tgctttgttt gccatggata
    47221 gattcacctc ctccacaaga gatatgatag aaatatcatt tagttaaaac tttctgagtg
    47281 gtccagaagt ttaaaaagaa atattgatta acttcaatat tctgttatca cgatatcata
    47341 tgaaccgctt aatagcaagg tttttaccaa ttttcacatg ctttttttag tcatagaaac
    47401 gtaacaaacg aaactgtctt tttcttttga aaaaaaaacg tcgatatggt aaaatgccta
    47461 ttttctccta cgagggataa ttcggatgga cgtcccttca aaagtaaatg cttttctgat
    47521 ttgattttct aagaaacgtt cataagaaaa atgcatcaat tcctcttcat tgacaaaaac
    47581 gacaaatgta ggtggtttga cagcgacttg tgtagcataa aaaatcttca ggcgttttcc
    47641 tttatctgtt ggtgttggat tgattgctac agcatccata atgatatcat tcaacacagc
    47701 cgaagagata cgaagattct ggttcatact tacttgttcg atcatttcag gcagacgttc
    47761 caaccgttgt ttagtcactg cagaaacaaa aacgattgga gcgtaatcaa gataccggaa
    47821 ttcatcacga atctcttgtt cgaaatcacg cattgtattg gtttctttct ttacaagatc
    47881 ccatttattc acgacgataa taattccacg accagcttca tgggcgtaac ccgctactcg
    47941 cttgtcttgt tcccgaatcc cttcttcagc attcagtacc atcaaaacga tatcagaacg
    48001 gtcaatggca cgcattgcac gcatcacgct gtatttttca gtattttcgt atacttttcc
    48061 gcgtttacgc ataccagctg tatctatcat taagaatttc tgaccacttt cagaaacgaa
    48121 gtgggtgtcg attgcgtctc gggtcgttcc ttcaatatcc gaaacaatta cacgttcttc
    48181 tcctaaaatc gcattgatca aagaggattt accaacattt gggcgaccaa tcaaactaaa
    48241 cttaatcgtg tcgtcgtcct cttcttcagt ctcttcagag aaatacttca ctgcttcatc
    48301 taaaatatca ccaatcccta gtccatggct tcctgaaaca ggataaggat cgcctagccc
    48361 taaggaataa aattcataga tatcgtttct catttctgga ttatctactt tattgactgc
    48421 taaaatcact ggtttattac ttctgtataa aattttcgca accatctcat ctgcgtctgt
    48481 cacgccttcg cgtccgctga ctacacaaat gattacatct gcttcttcaa tcgcgatttc
    48541 tgcttgatgc ttgatctgat ccataaaagg ttcatcgcca agatcaattc cgcctgtgtc
    48601 gatcacgctg aattctcgac ctaaccattc accttttgca taaatacgat cacgagttac
    48661 tccaggagta tcttcgacaa tcgagatacg ctcaccagcg atgcggttaa aaatcgtaga
    48721 cttaccgacg ttcgggcggc caacgattgc aattgttgga tttgccatat tatttcctcc
    48781 tcacaagttt ccaatctcac ttagtttaac gaatgcattg ccaactttca agcatagatt
    48841 gtcaattaaa cgaagtattt ttattcctta ttgaaatact aatcgctttt caaaagaaaa
    48901 actgagacaa aagcagctgc tttcatctca gtcacagata caaaatcaat tatttttcag
    48961 aatcatcggt tgctttttct tctacttctt ctttcaacgc atcacctaaa atgtctccca
    49021 tagtaaatcc agtattttct tctggtaatt cataagactc aacatttttt ggttcttctt
    49081 ctgaagctgg tttttcttct aaagctttga tactcaatgc aatacgatgt tcttctggat
    49141 ggatctctaa tactttcact ttcacttcgt caccttcatg aagcacttca tgtggtgtcg
    49201 caatatgttt atgtgaaatt tgagagatat ggactagccc ttctactcca gggaacactt
    49261 caacaaaggc accgaagctt gtcaaacgtt taactgttcc gtctaatact gtgccaactg
    49321 gagcttttgt atcgatttct gaccaaggtc caggctgtgt gtctttgatt gacaaggaaa
    49381 cacgttcttg ttcaggattg atagacaaca ctttgacatt tacatcatca ttgacagcca
    49441 atacatcact tggttttgct acatgactat gtgaaatctc tgagacatgg actagaccgt
    49501 caattccgcc taggtcgaca aatgcgccaa aatcagtaag acgtgcaaca cgtccactga
    49561 gaacatctcc ctcatggata gttgaaagaa tttctttttt cttttcttct ctttcgtttt
    49621 ccactaccgc tttatgcgat aagatcaaac gattttctga tggttcgatt tcgatgattt
    49681 taaagctcaa tgttttgcct ttatagtctg aaaagtctgc aacaaaatga tcttccacca
    49741 ttgatgcagg gacaaaacca cgtacaccga cgtcaacgac taaaccgcct ttgacaacat
    49801 ttgtaacagg tgcttcgatg accttgccct cttggaaatc tctttcgata tcttcccaaa
    49861 cttttttagc atctaaacgg cgttttgaaa gtagatagct gccgttttct ttatctttgc
    49921 cgatcgtact gataacgact aaatctaata catcaccgac ttttactact tcgctgatat
    49981 cttctaccgg caaagttgat aattcttttg ccggaacgac tccttcaact ccagcccctt
    50041 cgattccgac aatcacttgt ttgtcttcaa tgactaaaac ttcgccctta acaacatcgc
    50101 caacttttac ttcttgtacg cttttcattg cgtcttccat tgactggttg ttctcttgat
    50161 tttgatcaaa ttctgtcata acacttgtcc tcctaaccaa cattctgccg taagtacggt
    50221 actgttttta gtttaattga ttattcaaaa aaaataaagc gatttcgttc gttttccttg
    50281 aaaaaacaat tgtttttcat tttttttctt aatttaaaaa agaagataac cttttttcaa
    50341 aacaacagct tttattgctg caacgacttc tggaatcgat ttgccagtcg tatcgatacg
    50401 aacagcatcc tctgcttgct tcagcggaga gacgtccctt gttgaatcat aatggtctct
    50461 ttcctgtatc tctttcgtca gtgtttcaag atcagtcgga attccttttt cttggttctc
    50521 tttgaaacgt ctttctgcac gctcacttac gcttgctact aaaaagattt tcacttctgc
    50581 attcggtaat acagctgtcc cgatgtctct tccgtccatg acaacgccac cgcgttcccc
    50641 tatttttcgt tggacagcaa ctaattcttc tctgactttt ccatgtgcag agacttcaga
    50701 aacagctttt gtgacatctg gttgacggat ttctaaagtc acatcttttg atgctatgaa
    50761 tacatgctgt ccatcttctt gttgcgaaaa agagatagga tactttttga tcagcgaaac
    50821 tagcccttct tcatctgaaa aagatacatt gttcgtaata gctaaataag ttactgcgcg
    50881 atacatggca ccggtatctg tataaatata attcaattct tttgctaata tcttggcaac
    50941 ggtacttttc ccagatgaag caggcccgtc gattgcaata ctgatttgtg tcatgatata
    51001 actccttttt ctcagaaaaa aagccgcaaa gcgcggctat tattatttaa ttcttaattc
    51061 ttgaccaggg taaagcataa agttgtttgg atctaagcca ttcaattcaa acaattgatc
    51121 agtggtgatc cctgcacgct cagccacttg ttttggacct tcgcctgctt gaacagtcgt
    51181 tgttgaaccg gctgcagctg atgaagaagg tgttgtttct gctgcattgc tgcttgaatc
    51241 ccctgcttga taagttgcct cacttgagga agtcggtgtt gtctctgctg caatactgct
    51301 ttctgacgtt acacctgtat cttgagacac agtagattct tccgcgattg tactagattc
    51361 tactgttgaa gtctcagttg actcctcttt tgttgtactt gtagaacttt ctactactga
    51421 tgtcgaagtt tcagaagaag ttgctgctgt tgcattgttg cttgatccat ttctgatcca
    51481 gaaataagca cctgctggaa tagcgataca taagaccagt aaaatcaaaa atagcgaaag
    51541 aaataatgta tttcctttct tttgttgtct ctgagaactt cttgatgaaa cttcatcatc
    51601 tgtatcgtag attggttgtt cccatggttc ttgtgtttcg ttattttctg tagattgatg
    51661 tctatctttt ctgctcactg ttttacctcc taaactctat cgtgtgtatt ataccatagg
    51721 aaaaagtgtc ttgtcctaaa aaatcaatgt tccatgtttt ttttaaataa atttagtaaa
    51781 atcgttcccc aatgaagttg ttcagtctta gaaaaaagct tcctctttgg cagaagttct
    51841 gcttttgctc catcaatatc acaacaattc tgcggctttt cggagagttt ttcgccaaaa
    51901 taagctaaga tgaattttct gcggcagttt tcttcattaa tatagcgaag catctgctgc
    51961 aggcggaatt ctttttcttt ttcctgacgt ttgagccgtt ccacccaatt ttctggtttt
    52021 tcagactgct ggatcagctc catccatttt ttttgcagct catcaaaggg agcttgctct
    52081 gtggcatatt ccaaataaag ctcaaaactt tgtcgctgtt ctctagaaag ttgattaaag
    52141 aaataatgga tacgttcatc cccactttga taaaaaagaa ttgctgcact ttcttcctga
    52201 tctctgcctg ctcgaccgat ttcttgtaca tagttttcta gactgtctgg caaatcataa
    52261 tggaccacat aacggatatc agatttatcg atccccatac caaatgcatt cgtagcaatg
    52321 agaaactgca gctgattttt tacaaactgc tgttgaagtt gtcttctttg agctgcatca
    52381 agaccaccgt gatagtatcc aactgtaaaa cgttccctga ataaatgata caatcgttcg
    52441 acttcttttt ttgttgcaca ataaatgatt gccgctcctt gtgctctttc catgaagtgt
    52501 tcaagttctt gttctttttg ctgcgttttc cgaacaaaaa gcgagatatt ttgccgattg
    52561 actgattgtc tgatctcttt tggtggtcga tcaaatagta catgacgtat atctcttgct
    52621 actaaatctg ttgctgtcgc cgttagtgcc agtgtgaccg ggtttcctaa aagctttcgg
    52681 atttttccta actgttgata ttctggacgg aaatcaactc cccattgaga cacgcaatga
    52741 gcctcatcta ctacatataa cgcaatattt tgttttttca gatgctctaa aagagaagga
    52801 ttagtcaaca tttcgggact caaaaacaaa aatttatatt gcgatagatg acggaggacg
    52861 tatcttcttt cagattgcga aagtaaacta ttgaaagcag ctactcgttt ctcccctcgt
    52921 ttttgtaatt gcgtcacttg atcttccatt agagaaagca gcggtgacac gatgatgacc
    52981 agaccttcca ttaaataccc cgtcaattga tagcaaaggc tttttcccgt tcctgtaggc
    53041 aaaatagcga gagtatcttc tccatcaagc aacgcctgaa taatttcttc ttggccggga
    53101 cgaaatgaat caaatccaaa atattttttt aattcattta ttaacattct gtatcgcttc
    53161 ctttctcaat agatagattt gatacaaacg aaattctcca taatctaatt gtccatctac
    53221 attccattcg ctaaagcgcc actctcgcac ggaatcctta tttggttcca aacgttcgat
    53281 tgtttctgca gagagaatcc actcgtatgg aaaatcatca aaaaacaagg cccactcaat
    53341 aagatgatct gttactgttc ctctttttaa atgacggatt gccatgatct ggtcaattgt
    53401 ctgaccattc ataaacatct gcttggtgat catcatgctt tgattgaaat tttgctgtaa
    53461 taatgaatcg atcaaagcgt gccaaagcga atctggccgt ttttcgattt gtgctaacaa
    53521 aaggtggata catctgcttt gttgcagata aaccgctgac tcatcattgt tttttgccag
    53581 ctgataagga agcagccctg ttcgctcatt tccagataat tggttagcta aaaaatctgc
    53641 tgcttcttct ggtatctccg aaaacagctg caccaaactc tcatacgcag tatcaatcaa
    53701 aatttcacgt ggcaattggc tttcactaag ccattttttt agttggaaag tataaaaagg
    53761 acttgtctct gctggaagat agtcttgaat acccgttgcc agattagaaa ttacttgaac
    53821 agcaaattta atgagccgcc aactagtttc ccctgttctg ccatagcggt caaaacgcaa
    53881 accagtatgt tctatttgat gttcggacaa catatcagcc cctgcaggcg ttagctttgc
    53941 ttcattttca tttatttcga tccaaccttg ctgcgcaatt ttttgcaaaa tttgataaaa
    54001 cttatcctgc ttcaattctg gaaaactgtt atggataaac aaaagttcgt gggtaaatcc
    54061 ataaataagt actgaagtcg ttcgttttcc cacaaggagt tggtaaagag aactaatccg
    54121 cagcttgtgt cctgatgaaa atagcgataa aataaaaaca atcatattat tctccttaag
    54181 ttaggatggt tagaaatgtc actactatgt aaacttgtac ctgaacgctg tattgcatgc
    54241 ggtctttgtc aaatcgaagc gccagatatt ttcgattatg atgaagaagg aatcgtttta
    54301 tttgcccaag aaccagaggc tcatcaacag ttcgtgatgc cggaagaaga aacatatgta
    54361 aaaaaagcgt atcaaaaatg tccagttcga gcgattttat tagaaaataa aatataaata
    54421 taaacgaaac tttaaaaacg tacactaagt tctctaaact tgaaaataag ccgggatctg
    54481 tttctcttgt tccgtgaaat cccgacttat tttgaaagat tcatttgcta tctatagtta
    54541 attgactagt tttttcacgg accctatagg acccatttat attatttcaa ctttttcagc
    54601 cagttttcca atgtattgac gatcacagtt tgctggtctg cgttagaaat ttctgcctct
    54661 ccatctcctt tttgctcacc atagctgcca aaacctccat gatttccgcc attgattgat
    54721 acatactctg ttctttccgg taaatactgc ttagcttctt gataagcttc ctgattcaat
    54781 actttatctt ttgtcgccgt gatcgataaa acaggaacag ctgattctgt caatgttcct
    54841 tttttgtcgg gataacttgc catgaagaat acccctttta aatgtttgtc tgaatgattc
    54901 ttattgacat aacgacttgc cattacgcca cctaatgaat ggccaccgat cacataatcg
    54961 gaatttggaa aatcttcttt gaccttatcc gctttgtttc cgcccatcac tgctagatca
    55021 agtggcattt tgacaatagc gactgaaaag ccgttttttg ctaaatcttg cgcccaaata
    55081 ctataacttt ctggctctac gagagcacca ggataaaaaa taagtaatgg agactttcca
    55141 tgattctttt tctctccttc aaaaaataag taatcttctt tgttttttcc ttcttttacc
    55201 aacacttgcg cttctgacga tggttcgtac gtattttgct gtatatagta ataaccggct
    55261 ccaccgagta atcctaaaat gatgactaag ccaattagta ttcgtctaaa tattttcact
    55321 ttctcccacc ttttctatct gagtgacccc tttttcttaa tcaagtatga ttatacctta
    55381 aaaaaataag agagaaatgc aaactttcca gcatcaaata aaaaaagacg gaatatccat
    55441 ttgaatatcc gccctttttg tatgtttatt tttatttagc acataaacga tacacagctt
    55501 ctgcataaat ttccattgct ttatacatac tacttaattc ccatcgttca ttcgcctggt
    55561 gcataaagtc aggagtatca gggaacatcg caccgaatgc cacacactgg ttcatcgtac
    55621 gagcaaaagt agcgccgcct gatacaaacg gttcagtcat gtctccagta atatcgcgat
    55681 atgtccccaa tagatttttc actaactcac tgtctagtgg cacgtataat gaatcaagga
    55741 agtcaaattc ttcataagtc aacccatatt tttctgctgt tttagttaat ttagctacta
    55801 aatcatcttt cttgaatgta acagggattc tcatatctac accgattttt gtttgttctg
    55861 gtgtgatttc caaacttgca aagttcatcg ttagctcgcc ggattgttca tcttctgttt
    55921 tgcccacgac agcttcacca gttgcatttt cttgaactaa ttttcctaag aaattgattg
    55981 ggccaaaatc aaatacttct gaaagagcaa ttgccagacg agaaatagca ttcactcctt
    56041 gtgctgcatc tttcgcgtgg atacttttac caagaacgac gatgccttca ccttgttctt
    56101 cgaaatcgaa ttcgtgtttt ttcaatgctt cttttacttc agaaagcttt tctccagagt
    56161 aaggagctgc atcaggaaca acattcaacg ctcctccagc ttttacagaa aactcgttcg
    56221 ttcctggtcc tgtcaagtaa gcttgcaata agcctttttc tgcataaatt aaaggaaatt
    56281 cagcatctgg cgcaaatcct tgcgtaattc cttcttcttt ttcgttatat ttttctaaac
    56341 aacgccataa attttcttca tctgtaccaa agataaaacg aatacgtgtc ttgaattcca
    56401 cgccagcatc aatcaaagct ttgacggcat acattgccgc aatagaagga cctttgtcat
    56461 cttgggatcc gcgaccaact aaccagccgt cttttatagt tggatcgaat ggttttgttt
    56521 cccaattatt ttcgtcccct gctggaacaa cgtccatatg acacaagata ccgaataatt
    56581 catctccaga gccaatctct gaataaccgt aatagccttc tggatcttta aatgttctaa
    56641 atcctagctt ttcactgatt tccaatactt tatccaaagc ttcaatcact tttttaccga
    56701 agggatgtcc ttgtcctgaa tctgcttcat ctagtacgga aggaattcgg atcaattcgc
    56761 tcaatgattc taatgcttgt tcatgatgtt gttctgtcac aaattttttc atgatattat
    56821 cctcctaata tgttaaaaag ggtcatgtca aattcctccg acacaacctc ttcttctcaa
    56881 ataatgacgt tttaagcctg ctccatcagc aagcagaatg ttcttaacaa atgatgaaag
    56941 aaaaaacact cactgaatgg agctattgac ttatccacgt gcttatcgat agccgtttat
    57001 cggtttcttc attcttatga tagaccttgt acctgtattt gtctaggttt agaacattgc
    57061 tgctacacct aggatcacac agatggctac aaataatatc gcaattaatt taccggtaaa
    57121 tttccaccac actgaaacat cgaaacgagc aattgctaca gcacccatta cgatagcaga
    57181 tgttggtgta attaagttta ctaaaccaga tgcagattga taagcagtga ttactaaatc
    57241 cttaccgacg ccggcaaatt cacccatagg tcccatgatt cccattgttg cagcagctaa
    57301 gccggaagtt gaaggaatca agaatgacat agggatatag aaaataaatg taataatgat
    57361 gaatacactt gaagataatg aacttaatcc ttgttcgccc caatgtaaga tggtgtctgt
    57421 gattaatcca ttgttcataa ccacttggat accacgagca acagctacta cgattgctac
    57481 accgatcaaa tcagaagcac cagctaaaaa ttcagagaca aattcagact ctttcatgcc
    57541 ataagtgatt gctactacga tggacattac taagaataac atagtaattt ctgggaagta
    57601 ccagtcacct aatggaagca tgcttttacc tagaaccgta cctagtaccg gaaggtttgt
    57661 taaccagttt gtcaatgatt caaaaatcgt gaaattcgca ttcaaggttg tccaaggaat
    57721 caaactgatg atcatgatac caaaagtaat aaagaacaat acattaacat gtttttgttt
    57781 tttcgtgatt acttcttgac gaccgattgc ttcaatatcg aaatgcttca tatcttcttt
    57841 acgttggttg tatactaatg attttgttgg atctttttcg atctttgatg cataacgata
    57901 cacatataca atagaaatag ctaacaaaat aacgaacatc aagaatcgaa gaccaatacc
    57961 ttcacccatc gaaatcccaa cagcttgtga agctacgcct gttgcaaaag gatttaccgt
    58021 tgaagctaga cagcctactt gagaccctac taatacgatt gctacagcta ctactgtatc
    58081 aaagcctaca gccatcatga ctgggatcag taaagcatag aatggaatcg tttcttctgc
    58141 cattccgtaa gtagagccgc ctaatgcaaa taacagcatc aatactggaa taagcatttt
    58201 ttcttttcct ttgaatttct tcacaactga agcaatcccg gcatctagtg cccctgtttt
    58261 gttgataata cctaaaaatc caccaacaac caaaatgaag aaagagattg ggattgcagc
    58321 aggtgtttct ttagttagct cccccgctgg tacacctagc atcccattga ttggcgccat
    58381 gaaaatatcc cacagccctt gaggattttg ttgtactcgt tcatatgatc cagcgacgat
    58441 gttgcctgca tcgtctacac tatagtgccc agctggtata aaccaagtaa gtacagcgat
    58501 tactgcaata ataataaata agaccgtata cgctgaaggc atttgaaatt ttttcttttt
    58561 tgtttgttcc atcttcttct tcctttcttt tccgttagag acaacaattt tggctctaat
    58621 gttattttat caaaaagagt atcagaacaa aaataataac acgcattttg taaaactttt
    58681 gttcaaaaag ttcgtttttc cctattccct cctctttttt caggttttta aagctttctt
    58741 ctttttcatt gtagcgtttc ctttttttta taaacaaaca ttttcgtctt ttaaaaggag
    58801 aagaatttca catttgcaaa gaattgattg atttttatgc ataaatcaaa aaaatactca
    58861 aaacaaacaa ataaaacgtt tacattattg catgtcttgt atttttttcc aaaaaaaaag
    58921 accaagaaat ttcttgatct ttttgatatt tatttgactg ctgtatgacg ttgttcgaac
    58981 tgatgttgtt ttcgacttaa ccatggaaga agttgcgcat gcagcactaa aaagacagca
    59041 ctcacgatga atcctttgat caagttgaaa ggaacgatcc caatcagtac atagtttgct
    59101 aaagacatgc ccagcatctg tgttgcgttc agccctaaaa acattaagta aagaggcgta
    59161 ataacgaaat aatttgcgat actcataaaa atagttagag cgatggttcc tgtaatgaca
    59221 cctaaaaatt tgtttcttcc tttatgctgt ttctttacac taaagaaata ataaatcggg
    59281 aatgtaaaga tcgttgttga taagaaacta gcagtgtctc ctaccaaatt atccggagaa
    59341 aaacctgtag tcagcagatg taatactgaa cgcaagaacg ctgttgcaac tccggccgct
    59401 ggaccgaata agaacatgct gatcaatacc gagatatcac taaaatcgat cttcaaaaaa
    59461 ctaaaagccg gcatgatcgg aaaagcaaaa tactgtaaaa ctaatgcgat cgcagcaaac
    59521 atcgctactg caaccatttt ctgtacacgg gtgttcttca tttgaaatcc tcctgttagg
    59581 actcctcttc agcgaacttt ttgacgtttc tctccaaatg aaaaaactct attttccagg
    59641 gaaaatagag ttagagttat ttgatcaagc gtcgtcaaat aagctctatc ttctttatcc
    59701 agactatact gtcggtatcg gaatttcacc gattcagcta tttttttgca aaataggtcg
    59761 tggactataa ccaccggtcg ggaatttcac cctgcccctg aagacgaacc ttatataatt
    59821 tttgtttaca tattcattat acatatctat tcaaaaaacg caacatctaa cacttataaa
    59881 aagtaagtgt tcattttgag tggttcagca aactgctgac ttcttttttg ttcagttcac
    59941 ggtaatcccc aggacgtagg ccttgtaaag tcagatctcc gtatttttca cgttttagtt
    60001 tttgcacagg aagaccaact gcttgaagca tatttttcac ttgatgatta cgtccttcgt
    60061 ggatcgtcaa ttccacgata ctgtggtttg tcttaggatc cactgataaa atttggaatt
    60121 tagctggaga tgtttttcgt ccttcgatct ttattccttt tgtcaaaggt gcaagcatcg
    60181 attttgtagc gatccctttt acctttgcaa cgtaaacttt atccacttca tgttttggat
    60241 gtgtcaggcg ctgagcaaaa tctccatcat ttgtcagtag caagatcccc gaagtatcgt
    60301 aatccagtcg acctacagga tagatacgtt cttttaccat cggcaaaaga tccgtaacaa
    60361 cttttcttcc tttgtcatct gcaacagcag aaataactcc tttaggtttg tacaataaat
    60421 agtaaccgta ttcttcttga taaattggga cttcatccac ttccacagtg tcatgtttcc
    60481 caacttttat ccctaattcc cttacaactt gtccgtttac ttttacacgt cctgcaagga
    60541 tgaattcttc tgcttttcga cgagaagtga tgccagcgtg ggcaattact ttctgcaatc
    60601 tctccattac aattattctc ctttattcat tccatcaaag aatagatctg gtgacacgtc
    60661 ttcttcaagt gaaatttcca tttcttgtgt atcaggcagt tcttctaatg atttcaaacc
    60721 aaaataatct aagaagtagc cagttgttcc atataatatc ggccgacctg gtccttctac
    60781 tctacctttt tcttcaatca gtttatgggc agctagacgc tgtaccgctc ctgaaacctg
    60841 aactcctcga atctgctcga tttcagctct tgtgattggc tgtttataag cgatgattgc
    60901 taaggtttcg acggcggctt gagataatgt gtttgatgtc ccttgcgcat atcgcttaag
    60961 taagggagcc agctcttttt tagtcgttaa tacataatgg ctttccgttt caaaaatatg
    61021 caaagctgag tgcgagtttt gagtgtaaga ttcttttaat tctttcacta aacttgtgac
    61081 ttctgcttcg ctgagattta acagtccgct taattcttca ttactgatcc cttcctcgcc
    61141 aacaataaaa agaagggctt caagttcact taatttcgtc atgttcggta tctcctactg
    61201 ctggataaag cataattgat tcatagtttt ctttttgttg cgcaatgatc tgcccttttt
    61261 tcatcaattc tagcaaagcc ataaaagtag tgacaacttc acttttagaa ggtgactcaa
    61321 aaaaagaatc gaaagtacag ccatgcgcat ttctttttat acgctcttcg ataaatacca
    61381 ttcgttcttc gatcgttgtg tcatcgggcg tgatcgtttt ttccactttt tgagaaaatt
    61441 tcttacgttc taagattttc tgcatggcac ggatcaaatc gatttttttg acttgtcctc
    61501 gcttcaacgg cttttcagca tccgctaacc catcgatgtt cataggttct tttgtgtaat
    61561 aaagacttcg ttcctctgct ttttctgtca gtctttcagc tgcatattta tatttacggt
    61621 attctaaaag ctgagtgacc aattcttctc gaggatcttc ttcccataat tcttcatcgt
    61681 ctgtgacgaa ttcttggaca ggaagcaatg tcttgctttt gattgccatc aatgtagcag
    61741 ccatcactaa gtattctcct gcaacagcta gctcgagtgt tttcattgta tggatataat
    61801 tcatatattg ctccgtgatc gtagcgatcg gtatatcata tatatcgatt tccatttgct
    61861 gaatcagatg aagcagcaga tcgagaggac cctcgaatac atctaattta atattgatct
    61921 ccgtcaactc attcactctt tcttcttttt ctttgctagc cattttccaa tgtaaggctc
    61981 tgtgatttca gttgcacgga tggagacatc cgggtacttt tcctgtaatt catctaacat
    62041 ttgaaagccc atattctcac cgcgataaga cggattcaaa gaaatgtcat ggatgatcag
    62101 ctgatcgttc gttgcccagt aaataccaat aatcccttga atgttggttg aaccttctgg
    62161 cgtaaagaaa aaaagttcat aatcgtcttc agattcgtac gtatcaatct ccttcaacaa
    62221 agaagtttta cctgtcagtt tcttatggaa actcaatagt cccatagcaa ttttacggtt
    62281 ttctttccgg taatgtgtta acatcttctc accttcctat gactaagccc ttggaaaatg
    62341 ctgcttataa acatccgtca ttcttttctt cgtgatatgt gtataaatct gagtcgtaga
    62401 gatatccgcg tgacctaaca attcctgtac tgtccgcaaa tcagctccat tttctagcag
    62461 atgtgtggca aagctatgcc gcagcgtatg tggtgttact tctttgtaaa tcccagcctc
    62521 tcgtactaac tgcttcagat ttttccagat tccttgccga gaaagacctt tcccatgatt
    62581 attgacaaat acatgcattt cagatgcgtc ctttacaagt aatggacgtg cttcgtctaa
    62641 gtagcgttcc agccattgga tcgcataatc acctaaagga atgatccgct ctttatctcc
    62701 ttttccaact gtctgtacca gtccaagaga aagatgaaga tctcccaatt tcaatccaat
    62761 caattcactg acgcgcatcc cagtagcata catgacttcc aagattgcac gatctcttat
    62821 ccctaacgtt tttgtcgtat caggtgcttc aatcagcttt tctacttctg tcagagtcaa
    62881 tgtacttggt aatttctgtg ctttcttcgg cgtatcgata tgttgcatcg gatcatgatc
    62941 agtgatacgc tcttgccgta aaaactgatg gaatcttctg agacttgaga tcatccgagt
    63001 aacagttgct gtcgcttttt tttcttcatg caacttctcc agaaaacgga tgactaaaaa
    63061 gcgatcgaca tcctgccatg aagcaatttt ctgctcctgt agaaaatgca agtactgttc
    63121 caaatcacgt tcatagcttt gtcgtgtatt ggcggacaat ccccgttcaa tattcaagta
    63181 gtggagataa tcgttgattt gctctttcat aataatcccc ctcatgcctc tctatcatat
    63241 cagataccgc ttcattctga tagtcttttt ccataatctg agcaaagaaa aagccgtcta
    63301 gacactcaga cggactggtt tcttttttct ttttcatagc attcctgaca gattccatgg
    63361 aacgttagcc gatgatcttt tactaaaaaa tgataacgtt cttctactac ttgctccact
    63421 tcaagtaaaa gatcctcttc tacttcctcg atcgtgccgc agttcaagca tagtaaatga
    63481 tgatggaaat gtttggcgcc ttcttttctc aaatcataac gggctaatcc atcattaaaa
    63541 cttactttat cgacaatttt caattctgtc aagatttcaa gggttcgata aacagtagca
    63601 agtccaattt ccggactctt ttttttcact aaaaagaaga tttcttctgc agataaatgc
    63661 tctttctcat tttccaaaag cacaagcaga gtagcttctc tttgcggtgt cagcttaaac
    63721 cccgcttcat gtagctgctg ttttgttttt ttcatcgccg aaatagcatt catgaccatt
    63781 tactcaaccc caacaattta taataattac aaataaaaaa agatataaca ctatgttaat
    63841 taaaattatt ataagctagt taagaaaaac atgcaaggga ttctcaatta gattttttga
    63901 ctagttttgt cacaccgttt tcttgtgtaa tcaatccttg tttcaaaaga ttacctaatg
    63961 cgcgcttaaa tgctcctttg ctgataccaa aagctttcat gatttcttct ggatccgatt
    64021 tatctgtaaa gttgatttgc tgatctgcag aacgttctaa aatggttaaa atcatttgtg
    64081 catcatctga tatagcttca tatgctcttg gcttcaatga aagattcagt acaccgtctg
    64141 gtcggacacc aatcactcga ccttttatct gctcgcctag acgaggttca ttatatcgtt
    64201 cagaaggatg gataaaccca agatagaaat cttccgttaa aacgtacgta cctgttaatt
    64261 tcaagcggta tgctgttcct atcacatctt tgtttttcat ttcttctgtt ccatatttgc
    64321 ttaaagattt gaatattttt tcatctgcta aactgcccca aatacggtct tttgcatcaa
    64381 ctctaaggga aatcatcaag cgatcccctt ttttaggcca tagctctgtc attgtcggca
    64441 attcatcaag agatacgaca aagtctttat ctggaagacc gatatcgaca aacgcaccta
    64501 aatcttttcg tgtagcagta acgattccaa aagcgtaatg accgatacgg cttttaggaa
    64561 tttgtgtagt gatccgattt tcatgttttt gattttggta accgaatcct tcaactgctt
    64621 caccgatttt gtgttcacct tctgatttgc ttaagccaaa ggtttgtccg tttttttgga
    64681 caaagtagtt tttttcattt tcatcaataa taatacctgt aaatatagtt gctaataaat
    64741 cgttcatttt tttctccctc tatagtgaaa ggttttctta atacatttct ttgctatcat
    64801 aacataatgt tatctacttg aatagctgag ccttggtttg gaaaggtgaa aaaaggagta
    64861 tgacaacagt tgtgtcatac tccccttttt cgaataaacg gtgttaaacg gcaggctcac
    64921 agcctctttc atttgcctaa aattaattgc tcccatttcc tccgaaccat ccagttttac
    64981 agttgatggt tacgatgtaa aatggcaaag cactttctcc aatgcctttc atcaatgcag
    65041 attttgttga agtggcatat agattataat agccttcccc attttcgatt ctggcttttt
    65101 ctaatctgta acctccttca acgatatatc cacgttggat acatgtagca atcactttgc
    65161 tttggacacc tggtgcccac gtccatgctg gattagatgc atcggcaata tcacttgctc
    65221 tggcagattt gattggctga ttcgctgagt ttgaagagga aggggtcgag gatgccgctt
    65281 cttgctgtgc tgcttgtgcg accgcagctt gttgagcggc ttgagctgca gctgctgctt
    65341 gagcggcttg ttttgcttgt tcttcagctg cttttttcgc ttcttctgct tttttctttt
    65401 cctcttcttc ttttttctct ttatcagtga gcatcttatc tacttgtttc aattgttcat
    65461 tcaactgagc agatagatct gtatttttca ctttttctac tgcttctttt gccgtcttgt
    65521 attggtcgcg tgttgctgaa tccactactt ttccgtcttt aaagactata gctgtttttt
    65581 ctttagctgt ttgcaattca gcatattgat ttttagcttc tgtttgtgct tgttgaatca
    65641 attttccaaa atctgtgtta tccgtttcga ttgcttggat ctctttatct gctttcaact
    65701 tgatgtcatc tgctagatga tcatcgacga tggcaggtgc tatgaaccac tcattcactt
    65761 tttggatatc tcggatctta gattggatat cttggtaagt tgtttctaac tctgaatatc
    65821 ctttttcatt cttaagactg gataaatcat tttttagttt tgataaatct tgatgaatca
    65881 tatctgcacg aataaattga tggtgatcat ctgtataaaa ggcagcaagt ttctcatgaa
    65941 gttcttccat ttgttgctgt ctttttgctt ctgctgctac ttcggcttct actttttgat
    66001 gatggtcata ataagcccag cttcctccac cgagtagaag tactgctgct aaaacgagat
    66061 aaggtacttt ttttgatttt ttcttttgcg ctttatctgg atctgttttt tctaagtttg
    66121 attcttgatg tgttttttca gtatctggca cgattgttat tgatttcgct tctgcttcaa
    66181 acagcttagg ctggttctct tctttttcta tttttgcttc aagatcagta ccctctttgc
    66241 ttacagattg attttcagca tcggattcac tatttatttc atcgggctct gttgactttt
    66301 tgtctgtgtt ttcgagtatt tcttttttct ctgcttccgg cagtttattc gttttttcct
    66361 tttctaattg tttttcagta aatacttcag ttttttctgt gatatccgtt tttacgtctt
    66421 ttatttttac tgtttcttta cctgtggcgt tttctctatc agagtctttt tcagtttctt
    66481 gttccttttc tggctctact ttgttttcct gtgcaatttt ttcactgatc attttgagca
    66541 gctctttcgc gtgctcttct tttgtatctt cacgatgtgc tttgatataa gcggatagta
    66601 tcgaattttc ttcctcatta tcgacgattt ctttcagttc agctactctt cttttttgtt
    66661 ttgattcaag cgtttcttct tgattctttc ctgattcttc cgaagtctca tgaacagttg
    66721 ctttcttctg ttcatttttc tctatattag ttacttgatc gtgagcaggt ggttcttcgt
    66781 cttctggttc tgctttttcg aataattcca ttacagattc tagaggtaaa tctttcaatt
    66841 ccgaccattc gattggatcg tttaattctg tatcaggaaa gactgcatct tcagttgatt
    66901 tatcctgcat agctgcatct gtatttgttt cttcaattga ttcttttatt tgctgatctt
    66961 ctgcagttat ttgatgaccg cagtctggac agatagtatt ctgctccatt tttttcgatc
    67021 cgcaatttgg acattttttc atcacttagc gatcggctcc tttactaggt ttacgctacc
    67081 atttaatcta tcatattcta tacgtcacgc caatagctta tttttaggat ttacttaaaa
    67141 ataatgaatg aatttccttc ctatagaaaa aaaagcttga gactgcgtag tctcaagctt
    67201 tttttctttt aaaataaacc aaacagtttt actgatttct cttctggtca atagtccacg
    67261 cttattctaa gaaatctttt aattgttttg aacgagaagg atggcgcaat tttcttaatg
    67321 ctttagcctc gatctgacga atacgttccc gagtaacacc aaatactttc cctacttctt
    67381 ccaatgttct cgtccggcca tcatctaaac caaaacgtaa gcgaaggaca ttctcttccc
    67441 gatcagtcaa tgtatctagt acatcttcca attgttcttt tagtaactca taagctgcgt
    67501 gttctgctgg acttgttgct tcctggtctt ctatgaaatc acctaagtga gagtcatctt
    67561 cttcaccgat tggcgtttca agggaaacag gttcttgcgc gattttcaag atttctcgga
    67621 ctttttctgt tggaagatcc atttccgcac cgatttcttc tggcgtaggt tctcttccaa
    67681 ggtcttgcag caattgacgt tggatacgta tcaatttatt aattgtttca accatatgaa
    67741 ctggaatacg gatcgtacgt gcttggtcag cgattgctcg agtgatggct tgacgaatcc
    67801 accatgtagc ataggttgaa aacttaaatc ctttgcggta gtcaaatttt tcaactgcct
    67861 tcatcagtcc catgtttcct tcttggatca agtcaaggaa ctgcatgcct cgtcctacat
    67921 aacgtttagc gatacttact actaaacgaa ggtttgcttc tgccagacgc tgtttcgctt
    67981 cttggtcgcc ttcttcgatt ttcaatgcta acgctacttc ttcttccgca gttaaaaggg
    68041 agacacggcc aatttctttc agatacatac gaaccgggtc attgattttt acaccggttg
    68101 gcgcagaaag atcttctgtt tttgcttgtt cagctttttt ctcagcactc ttcaagctgt
    68161 gaattgatgg atcaccattt tcatccacca cactgatacc tgcgtcttca atcttttgga
    68221 tcaatttgtc catttcatct gcattcaaag tgaatggtgt agcaagctga ttggataaat
    68281 catcatatac aacttgtcgt ttgggtttgt tttctttgat gaaagctgct actgctgctt
    68341 catacgtttt tttatctgtt ccatttgcca taaaagaagg ccccccttga gtagctattt
    68401 ggcttgtttt aactgtttcg ttaaattgat gatttcaatc gtcaattcaa gctcaagctg
    68461 tttgtttcca gttcgtctgg cttcttgctg catttgtttt tttaatgtta tttcatcagc
    68521 taagccagat ttgctgatca catctagcaa gtcttggatt tctcgtggcg aactttcttc
    68581 tgccaccggt aaatattcga tttccaccac caaccgcctt aaattttcat cttgcaaaaa
    68641 gtctaaaaac tctgcaaggt tgaattcata atgttgtgag acatatgcat cgaataaaaa
    68701 ataaacttct gcatatgcat catgaataaa ctgaaattct cgttctttta aaatattacg
    68761 gacagaaact tctttaaatg cacgatataa tagaatctgc tctgcctttt ctacttgcgt
    68821 caaaggtctc tttttccgtg cagtttcttg tctcgtaacc tgagtgtgtt gactttcctg
    68881 aggttcttga cgattttgct gatttgtcct tcgaagatcg cgcagttgtt tttgtatcgt
    68941 ctctctgccg atctgaaact ctgttgacag ttgagataga tacatatctt gttcgattgg
    69001 tgaaacaact cggctaagtt caactagtaa ttcttcgagg tattcgatct tgtctttttc
    69061 attatctaaa tttttccctt gttttaaata acgtgattta aaagtaaaga ctgtttcgcg
    69121 cccatgagcc actaattctt ggaaagatgc ttctccatac ttacggagat attcatcagg
    69181 atcaagcttt tctggaacga ctacgacaga aagtgctaat cgactgtttt gccgaagcaa
    69241 ttcaatccct cgattggttg cttcaaaacc tgcgttgtct ccgtcatagc aaaagaccaa
    69301 ttctttcgtc agcttttcca tttgttggat ctgttgagct gttagacttg tccccataga
    69361 agctaatcca gttttgatac cagcctgcca tgcagcaatc acatccataa acccttcgaa
    69421 caacaaaatt tctccgtttt tccggatatc gcttcttgct ttatcaagat taaataaaac
    69481 ttgtctttta ttgaacagtt ctgtttctgg actattcaag tatttgggct gatctttttc
    69541 ctgccctttt tcaggtgcaa gccagcgtcc tgaaaaaccg atcgtctggc cttgagaatt
    69601 acgaagtgga aacatcactc tttgataaaa acgatcagac atacggccat catcatgcgt
    69661 gacaaacaat ccagagcgtt ctaacaattc atttgaaatt gattcattct tgaatacttg
    69721 ctgaagaaaa gaacgctcat taggtgcaaa acctatgttg aattcttcaa ttaattctaa
    69781 agacaaccca cgactttgca aatattctaa agctggctgt ccagcttttg tatgcaatag
    69841 catgtgatga tagatttccg ctgctttttc atgagcagca atcagtttcc ccgtagcaga
    69901 ttcaggattt cggacagcta tagtacgctg acgccattga tcttctaacg gtatctgttc
    69961 gaagtctgcc acttttacca ctgcttctgg aaaacttaga ccttctagtt cttgaagaaa
    70021 agtaaacacg tttccgcctt taccacaacc aaagcaatga aaaatctgct tgtcttctgc
    70081 aacagaaaaa gacggtgtac gttcttcatg aaaaggacac agccccaagt aatttttacc
    70141 agactttttc aactggacat attgtccaat cacctcaacg atattcgttt gactgcgtac
    70201 tgtttcgata acctcttgag gaatccgttg agccattttt tcaccccctg ctctattgat
    70261 atcttttggg tttaacagaa gatatttcag gagaataacc ggtaaaaaat cgcactaact
    70321 gaaatcagtg cgattttaaa ggacacaatt atacatgttt gattgtatca tatttcattg
    70381 ctttttcaag tataacacat ttctatctca ttaagcatca atttgctact cttttcagta
    70441 actgattttg aatatcagcc ttccagtaca atactcgaag aacaatattt acgagcatga
    70501 atacaaagaa gtatcctacg ctctctccca accctaagtt tacttccaaa attccatgga
    70561 acagcattgc agctacatat atccctacga tcgtcgttac tgctaggaag atgcgcaatg
    70621 ggttgaaggg ataacaagct tttacaactg ccatgcaact gatcccaatc agcaagtaat
    70681 acatcaaagt agtagtttca gcatttcccc atgcgaatgt ttttccaagt agatacaccg
    70741 caatgatatt cactactaca agcaaggcat ttggtaaagc attaataagt gctgtaggca
    70801 aataacggta agtgattttt cttttatctt gttcaaaaga taaaaagaag gaaggatacc
    70861 cttcaatcgc taaatcaatc agcgtgattt ggatagggat aaacggaaat ccaattgctg
    70921 taacggcaca tataatcgat aaaataaatg aataaatcgt tttgataaaa aagatgcctg
    70981 agactttcgt cacattgttg accactcggc gtccttcaaa caggacttct ggcaatgtag
    71041 taaaatcaga gtccaacaat accagattcg caatctgacg tgttgctcca tcaccttctg
    71101 ccatagcaat gctgcaatct gcttctctta acgccaaaac atcattcaca ccgtcgcctg
    71161 tcattgcaac cgttctgccg ctttctttca attcatttac cagcaatttc ttttgctgcg
    71221 gactcactcg tccaaaaacc gtatagcgat gagcagcttc tcgcacttcc atttcttcag
    71281 taatttgaga aagatcgaca tacgcatcat aattcggtaa tcctgcacgt ctagcgatat
    71341 tggagacagt aaaaggattg tctccagaaa tcactttcag atccacacct tcctcatgaa
    71401 gataagctag tgtttcttga gcgttttttc ggattggatc atcgatttca aacagagcaa
    71461 ccggctcaac aaatgctggt ttttcttcgt tcaattgctg atttggagcg atccccaaca
    71521 taagtacgcg atagccattc gcttgggctg tttttagact gtccggaagc cgatcttctt
    71581 ccaccaaacg ttcaggcgct cctacgtaca cgactcccag ttctggaaat tccatcgcgc
    71641 cccatttgcg atcagaagaa aatgcgactt tgtttgtcac atcataccga ttagaaagct
    71701 caaaataatc tctcatagcc cgcatagtga tgttgttatc actgctttct gccagatacg
    71761 tccccataat ttctgggatg gctttctcgt acagcggatg tagtgcttct acccgctgga
    71821 tactcatttt cccttcagta atggtccctg ttttatccaa gcataatgta tcgacatggg
    71881 ccagcgtttc aattgaatac atgtcttgaa caaggattcg tttttttgcc agtttggtta
    71941 cccctgtcgt taatgcaata cttattagca ataccaaacc ctttggaagc atgcctaaca
    72001 aagctgctgc agatgcaata acagaaattt tgatagaagc atcgcggatg aacagtgctt
    72061 ccaaaaatag tatgatcccc aaaggaataa tcacgaaact agtgaatttg gatactttgc
    72121 gaatcgaatc tacaagttca gaacgaatag ctttgtgagt ctttgcctcg ctagtcaagc
    72181 ggacagcata attatctgca cctactcgga taacttcagc catgacagaa ccgcttgtca
    72241 aaaagcttcc agaaagcaat tcagctccag tttctttgac aatcaaatca gattcgcctg
    72301 tcaaaagagc ttcatttgct tctgcccgtc cagatataac ctgtaaatcg gctggtatct
    72361 gttctcctgc tccgattttt accaaatctc ctaataccag ctctttagta gaaatcgatt
    72421 gttctttacc gtcccggatc actagtactt ctgattggga aacaatcgat aatttccgta
    72481 caagatttct tgcatgtatt tcttgataaa ttccaataac gatattgacg acaataattg
    72541 ctaaatacgc catgttggaa tacgcaccta caaataacag acaaatccca ataccaaagt
    72601 tcagaaaatt gaataacgtc aatatattat cgctgaatat ttgttttgta ctttttgatg
    72661 catcttcctg gtagtcattt tgctggccag aagccatttt ctgaactacc tcttgctgtg
    72721 taagtccatt cattttcttt ttctccattt tccgccaact ttcaaaaata atcacgtgtt
    72781 taagtctagc gtgttccaag attaaaaaca acttgttttt ataaaattta tagaaaaaca
    72841 cacgaatgat tttacaaaaa agatgccaac ctttttgttt gaatcacccg tgtgctgttt
    72901 ttattatcaa tctatcttta cttatattgt tcctccaaac ggctcatcca ccaacctagt
    72961 gctgtaccgg ctataaaaag aacaggacaa gctagtaccc cagcaactcc caatcccgcg
    73021 taatcggtag gaatgatcat aatcgttgaa gcaaaaacta cacctaaaat gaaatggaat
    73081 agcttgctat atgcctttga gaaaatgtaa tccatgattt ttgacaatcc aagaacacaa
    73141 agaatcccac caattccgat cggcacaatc acttcaagtc gtacttcttt aaatccgtca
    73201 gccatcgctt tatacaatcc catatacacc aaaaaattag aagggctcaa tcctgggacg
    73261 atcataccca aaccaatcaa tccgccggca atcagccatg ttccaaaatt ctgatcgact
    73321 tgcccgtcaa ataattgagc gccgtataat aaaaacaaat aagccagaac aaaagtaaca
    73381 accatgatga tcacatcaga agtatctctt ccttttttcc ctgcttcttt ccataacgca
    73441 ggaacagttc cagcgataca tccgacaaaa aaccataata tcgttgtttc atacgcacct
    73501 aataaaaaac tgactaaaaa agaaagtaga aagatgcctg tcactccgcc tagaccaaca
    73561 ggcagaaaaa acaaaacatt ttctttgaaa ttttttgtaa ggtgggctaa aaaagaaatc
    73621 agccgctcat acatgccaaa gacagctgct aatgcaccac cgctgactcc tggcaagata
    73681 aatcctgaac cgataaacat tcccttcaca aaccgtaaca tccagtcttt ggttgtgtac
    73741 cccacttctt gattttccat tttttgcctt ttttccacta tttgcgtact ccttctttca
    73801 tttaatttta ttcatgattg aacatttcct gctattttac ttaaagactt tttcataggt
    73861 ctctattatt ttttcattca ttttgacaat atcaggataa aacatgttac ctccattatg
    73921 atagagatag ccgccattgt aaagcaagga ctggatccgc caatatcggt attgtttccc
    73981 ttcctcattt cctaaaagtg ggctcagcac atctttagaa tacgcttctg ctaattctac
    74041 tgtgttttct ccgccatgat tttttacata ggcaatataa tctttcccaa aattatatgc
    74101 ttggacagct gtccataaat cgcatccttg tttctttgct tctactatca tttccgccaa
    74161 atatttgaca ccttgatcaa tactgtcagc tggattatca atgatctcag cttctccatg
    74221 cagactttcc gagctctgca tgagatctgt cccttttcct tttgattcgg tcaatattat
    74281 cgccttgatc aaattctcgt agttttccat atgataattt tttgcagctg ttgttatttc
    74341 tatttgatag ctttccactc gttttaccga acgataagtt cttcctcata aaaacaacac
    74401 cgcaccaata accgtcagaa tgatcatgta aaaaagaata cggataatag ataatcgttt
    74461 tcgcttcatt tcatcaccta gttattttga catctttttt tatcttacta tttctttatc
    74521 ttcttggcta tttcttcctt gatttctgtg tacgccttaa gaaaaaatga attttcctgt
    74581 attttttgac atcttacgaa aatgatcgtt gtttttcagt aaaaacaatt gcccaatgac
    74641 tgatataatc gttatacttt acacagtagg tgattatgtg aaaaaagaaa agaaatatgc
    74701 aaaaatgcca gatgggagcg aaatttacta tgaaaagagc ggtcaaggct tccctctctt
    74761 tttactgcat ggcaatgacg gcagcggccg ctttttttca gaacaggtcc ctgtactcga
    74821 acgctactat accgtgtatc tagtagatag ccgaggacat ggtcgttcga caaacgaagc
    74881 atctatgttg aattttcagt taatggccga ggatctgaat acgatcatgt tgctggaaaa
    74941 aatcgatcag gcagattttc ttggatttag tgatggagca aatctagcac tggtatttgc
    75001 cagcagcttc cctaaaaaag ttcatcgatt gattttaaat tctggaaaca cgcttgttaa
    75061 aggcgttcgt ttttctgcca gagtcatcag taatattcat tatgcatggg tatggttact
    75121 ctctttattt cgcccaagtc ttcgcaaaaa tcttttagtt atcaaacttt tgctgcatga
    75181 catcgggctt acagaaaatg atttaaaaaa aatcaactcc cccactttga tcatcgtggg
    75241 aaaaaaagat gtaatcaagc taaaacattc tttgtatata gctaaaacta taccaaaagc
    75301 ttcctttgtg cttgtgaaag aacaaggaca tgaattagct agaaaagatc ctgaacgctt
    75361 caatcgagaa gttcttcaat ttctttctga aacataacta ttaggaatag aaggtgattt
    75421 atttgttaaa aaataccata caatggctga aaaaccacct aggactgttc aaaacaatct
    75481 ttttgatttc tgtcattgtc attattgtcg gtgaattgat gtccatcggt aaaactctat
    75541 ctatccagca attaggagaa acgtttgcca ctatacctgt atggaaaagc ggactaatgc
    75601 tattgattgg tcttatctct gtgttaccta tggttggtta tgatattatt ttgaatcgga
    75661 tgcttgaaca aaagcagaat cctcgttacc tgtttgagac aagctggctg atcaatacga
    75721 tcaacaatat tgccggtttc ggcgggtttg ttagtgtggg gcttcgctct gaactatatg
    75781 gacaaaagaa agagagcaga aacgtgatcc aagcattatc gaaagttttc ttgtttttga
    75841 tggctggact ttctatttac agtctgcttt cttttttact tgtagtcttc acgccagttg
    75901 ctcctttttt gaagcaatat tggatatggt tgatcggcgg cggtctatat tttcctgtcg
    75961 tctttttatt tactactttc aaaaagaaag gattccttgg tggtacctca cttaaaactc
    76021 aaggacagct attgctagtt tcattgttgg aatggaccgg tgtcctgttt agtttccttt
    76081 ctgttggtta tttaatggaa atccggattg atctgtggca aacaattcca ttattcattg
    76141 ctgcttcagt gataggaatc gtgtctatga ttccaggaga aattgggagt ttcgatgtaa
    76201 tgatgattat cgggttgtcc gctattggtg tgccaagaga aatagtcgtt gtgtggatct
    76261 tgctctatcg cctgttctac tacatcattc cgtttttgat tggaatcgtc tttttcttca
    76321 aaaatatcgg ttccactttt gatcagcgtt attctggtat ccctaaacaa ttagcaacag
    76381 aaattgcgca taaaatcgtg gttgtcttat tatacttttc tggaatcatg ctagtacttt
    76441 ctgccactat cccgcaagct tttacagagt ttcgctggct tcatagtttg aacccattga
    76501 aatttcattt tattatccaa tttcctagta tactattggg atttctttta atcattatgg
    76561 gcagagggat cgcagcacgt gtgaaacgag catatttgcc tacaatcttc ttgattgttc
    76621 tagctttact ttatgtttta ttaagtgatt ttagtttcac accggtggtt ttcttgtcta
    76681 ttctgcttct gatcattctt gcttctaaaa acgaattatt tcgggaacaa ttgatctatt
    76741 catgggagtg gcgtacgatc gatggaatca ttattggtgc actgtccttg ctttacatcg
    76801 ttattggggt atacaatctg ccggatttcc ctcatcggcg tcaccatttt atttcctttt
    76861 tcttatttcc ttctgaaaag atttggtttt cgggattgtt ggccatcatc gctgttagtt
    76921 tcatgatcgt tctatttgtt catttccttc aaggagaaaa gaaacagatt ggcgaagcct
    76981 ttaatgaaga aaaggccctg aagatcctta caacttatgg aggaaactcc gatagtcaat
    77041 tgatcttctt aaaagataag cggatgtttg cctatgaaaa agacggtgaa ccaactgttc
    77101 ttctccaatt cgcttgcttc aataataaat gtattgtaat gggtgatcct tctggtaaga
    77161 aagaagattt tccagaagca atcgaagctt ttatcgaaga aacagatcga ctatgctatt
    77221 taccagtttt ctacgaaaca agcgaagaga ttgttatgat tttgcatgaa ttcggctacg
    77281 atttcattaa gatgggggaa gaagcttatg tagacctgaa tagttttaca acttcaggga
    77341 aaaaaatgaa gggcactcgt gctgtcttga atcggatcga acgagaagga tttacatttg
    77401 atgtcttaca accaccattt tctactgagc aaatgagtac tttcaaaaat atctctgata
    77461 attggttggg ttcacgaaag gaaaaagggt tttctttagg ctttttctct gaagattatc
    77521 tacaaagagc gcctattgca gttgtcaaaa atacccaaga agaaattgtc gcttttgcaa
    77581 cgatcatgcc tacttacacg aacaataaag taggaacgat tgatctgatg cgatatgatt
    77641 ctaaaaaagc accttctggc agtatggatt tcttgttctt gcatttgttt gcctatatga
    77701 aagaagaaca tatccaatgg ttcaatttag ggatggcccc tctttcaaat gtcggaactt
    77761 ccagaaagag ttttttacag gaaaggattg catacttggt ttacgagttt ggttctcatt
    77821 tttattcttt ccatggatta agagaatata aaagcaagta cgctacaaac tgggtctcac
    77881 gttatactct atattctaga gatagttgga ttgcatatgt tatgattgct ttgctgatta
    77941 ttgataatgc gccagttgag cagacacaag gtagaataag tggtttgaaa aaatggatac
    78001 gaagaaagta ttaaaaactg cataccgata gatgaacact ctcatgatga ataaaagaga
    78061 ctgaagtaaa acgaaagttc atgcttctac aatctcttct tatacaattg aagaacaaag
    78121 agcggtccaa acagaaatcc tgtttggacc gctcttttat tttaaacatt tttcattacc
    78181 aaaatattcg agtcgcgttt cttatgcttc gatctctgta acgatacctg aacctactgt
    78241 ccgtccgcct tcacgaattg agaatgtcgt tccgttttca acagcgattg gatggatcaa
    78301 gtcaacatca atcgtcacgt tgtcaccagg catgaccatt tctgtatctt ctggcaatgt
    78361 gattgttcct gtaacatcag ttgtgcggaa gtagaactgt ggacgatagt tgttgaagaa
    78421 tggagtatga cgtccacctt cttcttttgt caacacgtac acttctgctt tgaattttgt
    78481 atgtggagta atggaacctg gtttagcaag tacttgtcca cgttcgatat cgtcacgttg
    78541 gatcccccgt aacaacacgc ctacgttatc cccagcttcc ccgtaatcta acgttttacg
    78601 gaacatttct acacctgtta caactgcttt ttgtgtttca ggtttgatcc ctacgatctc
    78661 tacctcatca ccgacacgaa cagcaccacg atcaatacga cctgaggcaa cagttcctcg
    78721 acctgtaatt gagaagacgt cttccactgg caatagtaat ggtttatctg tatcacgttc
    78781 tggtgttggg atatattcat ctactgtatc catcaattcc ataatagcag cttcagcatc
    78841 tggatcgcct tgcaatgctt ttaatgcgga acctttgatc acaggagtgt catcgcctgg
    78901 gaaaccatat tcgctcaata actcgcgaac ttccatttct accaaatcga tcaattcttc
    78961 atcatcgact aaatcaactt tgttcaagaa aacaatcagg tattttacac caacttggcg
    79021 tgacaataaa atgtgttcac gtgtttgagg catcggaccg tcagttgcag aaacaactaa
    79081 gattgcacca tccatttgtg ctgcccctgt aatcatgttt ttcacatagt ccgcgtgtcc
    79141 tggtgcatca atatgcgcgt agtggcgttt ttctgtttca tattctacgt gtgcggtatt
    79201 gatcgtaatc ccacgttcac gttcttctgg agctgcatca atactagcat agtcttgagg
    79261 atttgctagg ccttttttgc ctaatactgt agtgatcgca gcagttaaag tagttttccc
    79321 atggtcaacg tgtccaatcg taccaatgtt aacgtgtggt ttacttctgt cataatgttc
    79381 ttttgccata atttacaaac ctcctaaata atatagatca ctgaaaaatt tcagctctca
    79441 cctttttatt atagtctttt tttgactaaa tcaaaaagaa tacgtccgac ttactttttg
    79501 taagattcga actaagctta ttatacgtca atattggtca aagtcaacta agagcactcg
    79561 tctgtcttat acgcgaaaaa agtacctttt ataaataaaa aaatgtgttt tttgagaaaa
    79621 acttcaagat agtccttaac tttcttttga agcaataaga acaaaatgag gaaaagttta
    79681 aaattcagct gttgccaaca actttcaata gacgaaatct tctatttcgt taataataag
    79741 gaaagaggtg aagtacatga aaaaattaat tggcgtactt gtcgttttag gcatatttct
    79801 cggcggaagt tttgcagctt atcattattt ttacggcggc gaagcttatt atacaaaaat
    79861 cacgacgaat ggcgaaatgt cagctaccca agcagataac ggagagaaat tcacgactta
    79921 tacctacaag caagcggctt ataataaaga tggtgaaaaa aaacaggtga cgcttcgcga
    79981 agaaagagaa catccattaa agatgaatgc gtatcttaaa ctcaaagtca atcctcgtaa
    80041 aggtgtatta agctgggaag aaataaaggc ttctgaagtg cctaaaaatg cggcagaaaa
    80101 aatcgataat tagtaaatgg tcttaaaatt ttattagata aaa
//
LOCUS       DAZKLM010000002        73767 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3764
            SAMEA115706913-rid22582563.denovo.002, whole genome shotgun
            sequence.
ACCESSION   DAZKLM010000002 DAZKLM010000000
VERSION     DAZKLM010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706913
            Sequence Read Archive: ERR13196647
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 73767)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 73767)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287823.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287823.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,004
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,849
            CDSs (with protein)               :: 2,849
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 41 of 96
            Pseudo Genes (incomplete)         :: 52 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 15 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..73767
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706913-rid22582563.denovo.002"
                     /isolate="VRE3764"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..976)
                     /locus_tag="ACG842_000076"
     CDS             complement(<1..976)
                     /locus_tag="ACG842_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coiled-coil domain-containing protein"
                     /protein_id="HFQ0246648.1"
                     /translation="MKKSLISAVMVCSMTLTAVASPIAAAADDFDSQIQQQDQKIADL
                     KNQQADAQSQIDALESQVSEINTQAQDLLAKQDTLRQESAQLVKDIADLQERIEKRED
                     TIQKQAREAQVSNTSSNYIDAVLNADSLADAIGRVQAMTTMVKANNDLMEQQKQDKKA
                     VEDKKAENDAKLKELAENQAALESQKGDLLSKQADLNVLKTSLAAEQATAEDKKADLN
                     RQKAEAEAEQARIREQQRLAEQARQQAAQEKAEKEAREQAEAEAQATQASSTAQSSAT
                     EESSATQSSMTEESSSATQSSATEESTTPESSTEESTAPESSATEESTT"
     gene            complement(1231..1740)
                     /gene="mreD"
                     /locus_tag="ACG842_000077"
     CDS             complement(1231..1740)
                     /gene="mreD"
                     /locus_tag="ACG842_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HFQ0246649.1"
                     /translation="MLKKETMKYYLPIVLFFLMLIDGHLTRMLGEWSKGTYMSNAHFL
                     ILALLCCSMAFEKRYLLITTIVLGAIYDAYYIGVIGIYAVALPLIVWLMYVMKDVIHV
                     NIFTEFFSMIIFVTGYELFTMVVQLIFKLAVVNNTYFITRFLGPTLLLNMIIFVLFIF
                     PFKKLFSDE"
     gene            complement(1744..2598)
                     /gene="mreC"
                     /locus_tag="ACG842_000078"
     CDS             complement(1744..2598)
                     /gene="mreC"
                     /locus_tag="ACG842_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HFQ0246650.1"
                     /translation="MKKFNPNKNIIITLVIVIIVVTILSVTAAQRANEGKPNFFQSIV
                     NDSVAMVDKTISAPVRWVRNGVDSVHNLFSTYAENERLKEKIDSYDDVSQQNKNYERE
                     IEALKKELELNQTLTSYEKVTANVITRSPDSWQDMLIVDKGSKDGIEANMAVMSQKGL
                     IGRVIEVNTASSKIELLSSSNESSNHFPVRVSSANGEAFGLLKNYDEKLHALVVTQLT
                     GDTDIKEGDVVQTSGLGGNSPANLPIGTVIKTKPDSYGLDREVYVKPYAEMYDVSVVT
                     IVQRLAED"
     gene            complement(2753..3391)
                     /locus_tag="ACG842_000079"
     CDS             complement(2753..3391)
                     /locus_tag="ACG842_000079"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004788 - thiamine diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HFQ0246651.1"
                     /translation="MKILLAAGGNPQNWPQSLLSKYDAYVGIDRGALYLLQAGYPVDL
                     AVGDFDSLSNEEQKEVFAKAKEYTRSPAEKDDTDTQLGILKTFERYPQAEVTLIGATG
                     GRLDHLLANLWLGMEPRFKPFAHQLIIRDCQNYLSYYLPGEHIVRQIPDMKYLAYCCL
                     TPVSNLTLKGSKYVLENKEVEYPTSYASNEFLSDTAEFSFSDGMIAVIQSKD"
     gene            complement(3392..4042)
                     /gene="rpe"
                     /locus_tag="ACG842_000080"
     CDS             complement(3392..4042)
                     /gene="rpe"
                     /locus_tag="ACG842_000080"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HFQ0246652.1"
                     /translation="MKIAPSILSADFANLQRDIELVEKGGADYIHVDVMDGRFVPNIT
                     LGPNIVQAIRPVTKLPLDVHLMIVDPENYIPAFAKAGADIITVHVEATPHIHRALQMT
                     KSLGVKAGVVINPGTPVSMIKHVLPLADQVLVMTVNPGFGGQSFIEETVEKITELADL
                     RRENNWTYAIEVDGGIVPETAKICKDAGADVFVAGSYIYGAENPVGQIERLKEALD"
     gene            complement(4055..4954)
                     /gene="rsgA"
                     /locus_tag="ACG842_000081"
     CDS             complement(4055..4954)
                     /gene="rsgA"
                     /locus_tag="ACG842_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HFQ0246653.1"
                     /translation="MALIKGQIRKAISGFYYVYANGETYQTRGRGNFRNRKITPLVGD
                     QVIFESENQTDGYLLEILPRKNQLVRPPVTNVDLGIIVTSIVEPAFSYNLLDRFLVTL
                     EYESIEPIIFLTKTDLSDDPEKIEEIKQTYTKIGYPVIVPNHTGDTTELIRYFPERLS
                     VFMGQSGAGKSTLLNQISPELNLATGEISDALGRGRHTTRHVELIPIYGGLVADTPGF
                     SSIDFLTIEAGELPRQFPEFVEASVHCKFRECMHAKEPGCEVKHRVETGGIAQTRYDN
                     YLQFLQEIEKRKPMYAKKEGKRK"
     gene            complement(5119..7188)
                     /gene="pknB"
                     /locus_tag="ACG842_000082"
     CDS             complement(5119..7188)
                     /gene="pknB"
                     /locus_tag="ACG842_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HFQ0246654.1"
                     /translation="MIELGKKLNGRYQITGNIGSGGMANVFLAHDLILDRDVAVKVLR
                     FDFQNDQTAIRRFQREALAATELVHPNIVSVYDVGEEDNMQYLVMEYVKGMDLKRYIQ
                     THYPVPYETAVNIMQQILSAISLAHNHQIIHRDLKPQNVLIDNEGVVKTTDFGIAIAL
                     SETSITQTNTMLGSVHYLSPEQARGSMATKQSDIYALGIILYEMLTGSVPFDGESAVT
                     IALKHFQDDLPSIKALDPNVPQALENVILRATAKEPADRYKSAEEMSDDLSTVLSPAR
                     ANEEKWQPHVMDNETKVITPLTEDAPMPDSFKSMPLPKDKPENETLPEEEEVLPDPKK
                     KGQKKWWLILLAILAVLGIGTAVFFASGGRGEVVVPDVSDLSEASARETLAKAKLEVA
                     TKTEEIADDKIEEGNVVKTDPAAGTTVKQKREVTLYISSGTKKIKLDDYSGKSYEDAE
                     ASLKELGFSADLIQKEEEFSSEVESGMIISQSPSEDTEVDPKTDTITFTVSKGPETVT
                     LSSHVGENYETVRNWLVTQGFNINNISATYDYSDTVPSGSIISQDPASGEVVAEETYI
                     NFVVSQGTEPKLNDISGYTKSEAQSYLASVGAEYIGHETSEYSNTVDKDKVIRTVPGA
                     GTTITKGMVVNVIYSKGPDPASSSSEKDSSDSSSSSSSSSSTSNSSKEESSSSTSSSA
                     STSESSK"
     gene            complement(7185..7925)
                     /locus_tag="ACG842_000083"
     CDS             complement(7185..7925)
                     /locus_tag="ACG842_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HFQ0246655.1"
                     /translation="MQIEYQSDVGRRRNTNQDYASVFTSRSGLKLAVLADGMGGHRAG
                     DIASQMAVTNLGEAWEKQEAVDDEKIAQWFIQSIQEENMLIYQRGQEQPEYNGMGTTI
                     VAAALSDDWFTIANVGDSRAYLIREDKIMQLTEDHSLVNELVKSGEISEEMAVNHPRK
                     NILTRSVGMPGTVEVDVSTHLWQLKDRLLLCSDGLTNMLSEEEIGTIINQEGPLSHKV
                     TELIDRANEAGGADNITVLLIEYKEDSV"
     gene            complement(7938..9296)
                     /gene="rsmB"
                     /locus_tag="ACG842_000084"
     CDS             complement(7938..9296)
                     /gene="rsmB"
                     /locus_tag="ACG842_000084"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HFQ0246656.1"
                     /translation="MKKIPKKMKKSVRYMALLTLERVNKGGAYSNLLLNEMIKQSELN
                     QKDVGLFTELIYGTISHQRLLEFYVAPLIAKAKKVDDWVKMLLYLSVYQLEFLDKVPA
                     HAILNEAVEIAKVKGNPGTGKFVNGVLRNYQRQGAPDLQGIADPIERLSVKISLPLWM
                     TKRLVDQIGYEETEKLGLSLYQPSHASARVDTRRLSRDQAITVLQEEEITAEKSQVSP
                     YGIVAKKGHLASSSLFHDGVMTIQDESSMLVAPSMQIEPEHHVLDACAAPGGKTTHIA
                     SFLDPSCGGEVVALDVHEHKVKLIEDNAARLGVSSAVYAQKMDAREVSKQFEAEQFDR
                     ILVDAPCSGLGLLRRKPDIRYKKQPDELANLPKIQLEILESCAPTLKSSGILTYSTCT
                     ILKEENQDVVSAFLERHPDFERIDVLTNPMLQSSIEEKMLTLYPHQFYTDGFFICCLR
                     KK"
     gene            complement(9296..10234)
                     /gene="fmt"
                     /locus_tag="ACG842_000085"
     CDS             complement(9296..10234)
                     /gene="fmt"
                     /locus_tag="ACG842_000085"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0071951 - conversion of methionyl-tRNA to
                     N-formyl-methionyl-tRNA [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HFQ0246657.1"
                     /translation="MTKLVFMGTPAFSVPILEGLIESGYEIQAVVTQPDRPVGRKRVI
                     TPTPVKKAALKHGIRVLQPEKISGSPEMEEIIELVPDVIVTAAFGQFLPEKLLQVPKL
                     GAINVHASLLPKYRGGAPVHYAIMNGEKETGVTIMEMIKKMDAGGIYAQESIPITKQD
                     DVGTMFEKLSLLGRKLLLETLPNILDGQKPVPQDESEATFSPNITREQEAIDWHKTAE
                     EIDNQVRGMRPWPIAFTTYEGIRWKLLSVEVLAETTDKAPGTIIRKDKKNLWIACGKQ
                     TVLAINELQPAGKGKQAVHDFLNGSGQQVQIGQQVK"
     gene            complement(10227..10718)
                     /gene="def"
                     /locus_tag="ACG842_000086"
     CDS             complement(10227..10718)
                     /gene="def"
                     /locus_tag="ACG842_000086"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HFQ0246658.1"
                     /translation="MRYPILIHPNDKLKRTAQPIDVITDETIALLDNLYETMIANDGI
                     GIAAPQVGQNKRIAVIEVDEGEKFELINPEIIEAKGESLDVEGCLSIPHVYGTVKRAD
                     EVTVRYYDRDGEEIEVTAFGYLARAFQHEIDHLDGILFIEKMIQQIPEEELEEYMEEH
                     LDD"
     gene            complement(10733..13141)
                     /gene="priA"
                     /locus_tag="ACG842_000087"
     CDS             complement(10733..13141)
                     /gene="priA"
                     /locus_tag="ACG842_000087"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HFQ0246659.1"
                     /translation="MTLTAEVIVDVPTMQTDQPFTYLVPSEMETALQVGMRVEVPFGN
                     GNRHVQGFVMAIQKSEESANPSLKAVIRLLDLAPVVNEELLSLADYMKKITYAFKITC
                     LQTMLPSVMKAEYDKLIYPLADTPEVQVLFDQREVISWKEAEEEGSLSQLIRWRQEQL
                     VDIKYEVHTRNKVKTIRLVRSLLTEKQIEEEWAKLRQNAKKQKELLLCLSELSQEEPI
                     AYFKNKGISTAVLNQGKEKGWLEFIESERYRDPYKDRVFDQTTALELNAEQKNAVEQI
                     ITAGQQQKDQVFLLEGITGSGKTEVYLQAIADVLSENKTAIMLVPEIALTPQMVERFK
                     GRFGESVAVLHSGLSQGEKYDEWRKIEREEAQVVVGARSAIFAPLKNIGLIIIDEEHE
                     SSYKQDETPRYHARDLAIWRSKYHHCPIVLGSATPSLESRARAQKGVYQLLQLNHRAK
                     AAAQLPAIEIVDMREEFQNHRTSTFSANLQEKIQNRLDKKEQTVLLLNRRGYSSFVMC
                     RDCGFVLPCPNCDISLTLHMDTRSMRCHYCGHEEPIPNRCPNCGGNKIRYYGTGTQKV
                     EEELRELYPQARILRMDVDTTRRKGAHEQILQKFGAKEADILLGTQMIAKGLDFPEVT
                     LVGVLNADTSLNLPDFRSSEHTFQLLTQVSGRAGRAEKAGEVVIQTFNPQHYAIELAK
                     KQNYEQFYQQEMHVRHRGGYPPYYFTVKITASHPEEQVAAKKIFQIANQLKEVLSPQS
                     LLLGPTPSMILRVKNRYYYQLIIKYKHEPNLPHVLDEILNGSQKEQRQGLFVAIDNEP
                     LNFI"
     gene            complement(13394..13699)
                     /gene="rpoZ"
                     /locus_tag="ACG842_000088"
     CDS             complement(13394..13699)
                     /gene="rpoZ"
                     /locus_tag="ACG842_000088"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HFQ0246660.1"
                     /translation="MMLKPSIDSLLDRVNSKYSLVILASKRAHELDASAQPTLDSFDS
                     VKSVGQALEEIDAGNVVNDPHPELKRERLKMEEEERQAQKEREQHELESRIREEQKM"
     gene            complement(13704..14318)
                     /gene="gmk"
                     /locus_tag="ACG842_000089"
     CDS             complement(13704..14318)
                     /gene="gmk"
                     /locus_tag="ACG842_000089"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006163 - purine nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HFQ0246661.1"
                     /translation="MSERGLLIVLSGPSGVGKGTVRKAIFESEDNDFQYSISMTTRKM
                     REGEVEGVDYFFRSKEEFESMIEAGEMLEYAEYVGNYYGTPLSYVNKTLDEGKDVFLE
                     IEVQGAKQVKEKVPDGVFIFLTPPDLAELKSRIVGRGTDADEVIEKRMKVAKEEIEMM
                     ALYDYAVVNDEVPLAVERIKNIIASEHFRVDRVIGKYIKMLKEM"
     gene            complement(14805..15089)
                     /locus_tag="ACG842_000090"
     CDS             complement(14805..15089)
                     /locus_tag="ACG842_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0246662.1"
                     /translation="MKYYDVTFHELSGKTVVKRDIPSEKNGFDVWKDACADYNENELF
                     ILINDGAYVTMNRKFIVRIDTEEVEDPTEKARSRKDEIMGVVNTLSNMGF"
     gene            complement(15107..15997)
                     /locus_tag="ACG842_000091"
     CDS             complement(15107..15997)
                     /locus_tag="ACG842_000091"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HFQ0246663.1"
                     /translation="MKSMTGFGKAIRETKAYQLDIEIKSVNQRFLDVQIRSPKILNFV
                     ENDFRQLIKQQLSRGRIEVFVNLAYIGKNEKQIVINWELIDSLITQLTDGIQQRYGSQ
                     NQVSISRMAERFVLDENFVTVEEKPEESMEELAQLALDTLKSALDSIEASREKEGQAL
                     ALVLKKNTEEFKEVLKKLDSFVEIYEKEFQIRYQKKLEEFLGAKVDEQRLLTELAILL
                     ERGDIHEELDRLAIHVQKLDELIEAQCPVGRELDFLIQEMNREINTIGSKSNAIEIKN
                     QVVQSKTILEKIREQVQNIE"
     gene            complement(16017..16235)
                     /locus_tag="ACG842_000092"
     CDS             complement(16017..16235)
                     /locus_tag="ACG842_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbC family protein"
                     /protein_id="HFQ0246664.1"
                     /translation="MAQEFSYEIVEEIAILSENNKGWRKELNLVSWNGRPPKFDLRDW
                     APDHEKMGKGLTLTNEEFEQLQKAIENM"
     gene            complement(16205..17905)
                     /locus_tag="ACG842_000093"
     CDS             complement(16205..17905)
                     /locus_tag="ACG842_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HFQ0246665.1"
                     /translation="MEIKKVYFSQKMSLYSPIQQAKQPLPFFQSHQESRDAFWRSAKK
                     QCAFKAVSKKYTVYFTIAKSRGDVIFNNLLIEGETFEWKKFFRYMEACARFFIKENCC
                     FLFQSPLSEEWLRIFHKNGYQGTTQLNKKLVYHTALVLGGGGAHGAYQIGVWQALKEH
                     DINFEIITGTSVGALNGALILQGDMKKAVNLWKKLSTRQVLALPEMAAVEDLRERFYR
                     ERRQMTKTALIEGGVSSAPLEKMVKDNLDLSLLKHNPKIRLYTVSTKLPELAEVVTDI
                     QQTKTEEIPDWILASASFYPAMAYRKIGKNKYADGGYRNKIPIDIAINKGATEAFVVD
                     VQGPGPAKKIRMPDIFIHWKCQTLWTLGSFLLFDSQRNQLNLQLGYLEMKKRLGYYYG
                     NWYTFHSVKPARTYWRGFLSYLTKEIQLELSFFKSIKFWQKLRNLYKNRVVPETCGLA
                     MLELLAKKHFLLPNEIYQVDMMIQMICQQDIKSMPDDLLAQIGQLSTEEWRRYRKYQQ
                     KIQNERTKTVYFDYLLQGKRKDRLQHELLKQPVDTLLILYLYYLKEEQPWHKNFHMKS
                     "
     gene            18172..18945
                     /locus_tag="ACG842_000094"
     CDS             18172..18945
                     /locus_tag="ACG842_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0246666.1"
                     /translation="MTESVYVHIDTTSNAVLTKGLTATDFANSIVHFPQNLLLLDPSA
                     SAGEYESHTGLKVIRGTENIQRFFSSSRNRRGFDELKWIDFTDLTMLKELTPLEISEL
                     LYFGHMKTHLHSPFFYKLQNNFVYFDLNDQLNRIYYRYLDEFYRIFASKLSSILSDKI
                     NVKKSFFKKETPVEKISVELLKEMRNLMQEGIIFSFDQVGLIDGKYRIPIYVIEDYIW
                     KLKGTRYREEEIIATLIYSVTDRHWQLVEDADLSYLPLK"
     gene            complement(19026..19595)
                     /gene="thiT"
                     /locus_tag="ACG842_000095"
     CDS             complement(19026..19595)
                     /gene="thiT"
                     /locus_tag="ACG842_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015234 - thiamine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015888 - thiamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupled thiamine transporter ThiT"
                     /protein_id="HFQ0246667.1"
                     /translation="MGKNRVWVEGTIVAALAMVLSLIPIQIGSSFSISLGQIPLTLFA
                     LRRGTKAGMLAGFIWGILHFPLGQVYYLSVVQVLIEYPIAYTFAGAAGLMASNVQKGY
                     VDDNTKQIRKSIVFGALIGALSRYFWHFIAGVVFWGAYALWGMNPWLFSFVMNGASGL
                     STAIVTIIVLLGIQQTTPSIFVPDKRQEL"
     regulatory      complement(19661..19763)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(19769..20740)
                     /locus_tag="ACG842_000096"
     CDS             complement(19769..20740)
                     /locus_tag="ACG842_000096"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016618611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HFQ0246668.1"
                     /translation="MSKHYFDPRLKIFALNSNRPLAEKIAAAVGVELGKSSVTQFSDG
                     EIQVNIEESIRGSHVYVIQSTSSPVNDNLMELLIMIDALKRASAKTINVVMPYYGYAR
                     QDRKARAREPITAKLVANMIQKAGATRMLTLDLHAVQIQGFFDIPVDHLMGAPLIANY
                     FLEKGIKGDDVVVVSPDHGGVTRARKLAEFLKSPIAIIDKRRPKANVAEVMNIIGQVK
                     GKTCVLIDDMIDTAGTITLAANALKEAGATSVYASCTHPVLSGPALQRIEDSAIERLV
                     VTDSIYLPEDRKIDKIDEVSVGGLMGDAIKRIHENKPVSPLFETKNK"
     gene            complement(20834..22207)
                     /gene="glmU"
                     /locus_tag="ACG842_000097"
     CDS             complement(20834..22207)
                     /gene="glmU"
                     /locus_tag="ACG842_000097"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006048 - UDP-N-acetylglucosamine
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HFQ0246669.1"
                     /translation="MDARYAIILAAGKGTRMKSKLYKVLHPVSGKPMVEHIINRVSET
                     KPDEVITIVGHGAEQVKAQLGERSKYALQAEQLGTGHAVLQAASFLEGKKGTTLVISG
                     DTPLLTTETLNNLFEYHQGKNASATILTAQAENPTGYGRIIRDHIGIVEKIVEQKDAT
                     PEEALVQEINTGTYCFDNEALFDALSKVGTNNAQGEYYLTDIIEILKEEGHTVAAYQT
                     DDFEESMGVNDRIALAKANEIMRKRINQMHMVNGVSFVDSATTYIDAGVEIGPDTLIE
                     AGVQIQGNTVIGSDCVIGSHSKIVDSRIEDHVVIENSVIESSHVKKHADVGPYAHLRP
                     KAEIGENVHIGNFVEVKNAQIGKGTKVGHLTYVGDATLGEEINVGCGVVFVNYDGKNK
                     HHTTVGDHSFIGSSTNIIGPVEVAKNSSIAAGSTITDNIPEYALAIARARQVNKEGYA
                     KKLPYLN"
     gene            complement(22710..23528)
                     /gene="purR"
                     /locus_tag="ACG842_000098"
     CDS             complement(22710..23528)
                     /gene="purR"
                     /locus_tag="ACG842_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0045982 - negative regulation of purine
                     nucleobase metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HFQ0246670.1"
                     /translation="MKVRRSERLVDMTQYLLNHPHELISLTSFAERYESAKSSISEDL
                     GIIKKTFKERGYGTLETIPGAAGGVLFIPEISYEEAKKYIESLAERLSEQDRLLPGGY
                     VYLSDLLGEPELLRQVGRIIASKYLGEKIDAVMTVATKGVPIAQAVSYYLNAPFVIVR
                     RDSKITEGSTVSVNYVSGSSERIEKMELSKRSLKRGSRVLVVDDFMKGGGTVNGMKSL
                     IEEFESELVGITVFAESKFNGHRAIDDYTSLLYVKDVDTQTKNITVVPGNYFSE"
     gene            complement(23695..24519)
                     /locus_tag="ACG842_000099"
     CDS             complement(23695..24519)
                     /locus_tag="ACG842_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HFQ0246671.1"
                     /translation="MALLSYEFMRRAFLAAIFIAGIAPMLGVFLVIRRQSLMADTLSH
                     VSLAGVALGFFLNLNPTLTTMLVVVLAAVILEYLRTIYRTYSEISIAILMAGGLALAL
                     VLMNLSGGNSATSIQSYLFGSIVTITWEQVIFLGILFVAIGILFFLFKRPMYVLTFDE
                     DTAHVDGLPVHLMSMMFNVITGVAIAVMIPIAGALLISAIMVLPAAIGMRLGKSFNVV
                     IAISVCVGFLGMLSGLTSSFYLDTPPGATITLVFIALFLIVNIIKRILVAFRRKNN"
     gene            complement(24492..25172)
                     /locus_tag="ACG842_000100"
     CDS             complement(24492..25172)
                     /locus_tag="ACG842_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HFQ0246672.1"
                     /translation="MRYIEISDLTFYYDDEPVLEDVSYHVDAGEFVILTGENGAAKST
                     LVKASLGLLKPSKGMIKIAEKNQEGKKLSIGYIPQQVASFNAGFPSTVIELVRSGRYP
                     RGRWFKRLSEKDHQHVQRALEAVGMWDMRNRRIGELSGGQKQRISLARIFATDPDLFV
                     LDEPTTGMDEESRNEFYRLLRHSAHVHGKSVLMITHDHEDIKQYADRQIRLVRKEDSQ
                     WRCFHMNS"
     gene            complement(25195..26133)
                     /locus_tag="ACG842_000101"
     CDS             complement(25195..26133)
                     /locus_tag="ACG842_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HFQ0246673.1"
                     /translation="MKKYLLTATVMIGALLFAACGNTNKEADKKEDLTIVTTFYPMYD
                     FTKEIVGDEGNVKLLIPAGTEPHDFEPSAKERAEISDADVFVYNSSDMEFFVDSLKDS
                     VDSKQTLMIEAAKGIDRLESQEADEHEESEEGHGHSHEYDPHVWLDPVLAIKEVRTIA
                     EELGEKYPDKKETFTKNADAYIKKLEALDQKYSDELKDATNRTFVTQHAAFAYLANQY
                     DLKQVAISGVSPDQEPTPSRLAELKEFVKKNNIKVIYFEENASSKVAETLSNETGVKL
                     EVLNPLESLTNEQIKAGENYISVMEKNLKALKESIN"
     gene            complement(26378..27229)
                     /gene="ispE"
                     /locus_tag="ACG842_000102"
     CDS             complement(26378..27229)
                     /gene="ispE"
                     /locus_tag="ACG842_000102"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HFQ0246674.1"
                     /translation="MELIEKAPAKINLGLDVLYRRQDGYHELEMVMSSVDLADHLFFE
                     EITEDKIIVETNKAFLPVDERNNVYQAALLLKNKYNIKTGVKIFVKKNIPVAAGLGGG
                     STDCAAALRGINQLWNLGCSLEELAEIGLEVGTDVPYCVYGSTAFVGGKGEEIQVLPS
                     MPQCWVVLVKPRMSVSTRTVFREVDTQAIQHQDIHGLAEAIREQDYQQMIKKMGNSLE
                     DITGKRHPVIHQIKERMIKYGADAALMSGSGPTVFALCQHYSRAQRVYNGLKGFCEEV
                     YLVRTLQ"
     gene            complement(27383..29956)
                     /gene="mprF"
                     /locus_tag="ACG842_000103"
     CDS             complement(27383..29956)
                     /gene="mprF"
                     /locus_tag="ACG842_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HFQ0246675.1"
                     /translation="MKNKRYLMLQWIKEHSLILKLIFFGSVLVFVANQVANIANGMSW
                     QDIWQRMGQQSRMTILLMILAGLLGVTPMLLYDWVTIRVLEKQGKPKMERKEFLLAAW
                     VTNTINNLAGFGGVVGASLRASFYGKQTNRKMVLATVSKVALFMLTGLSVWSFLTFID
                     VFFIQSESIFRSYWIWLFGGSFLAPVIFFFAYLKRKTLFFEFYPKGLLGLLLASFGQW
                     TGALAVFLIIGKLMRIDISMLSIYPMFMIATLIGMLTMVPGGMGTFDVLMILGMSQLS
                     VRQDVAVVWLLYYRLFYYVLPFMTGLLLFITHTGVKINRFLDNIPRLFFQKVAHIVLV
                     SAVYFAGIMMVLLSTITNLSAISRLFQVLLPFSFNFLDQTVNLLVGFLLLGLARALSV
                     KVKKAFLPTIALLSFGILNTITRTLSWQLILVYVLILLAVWLSRKEFYRTKFVYSWGA
                     IIFDASLFGFLFIVYAIAGYHSGRFLVGRITGNHFILFPSDDVWFSGLIGLGISLLAL
                     VALYQYLAEDTTKLGSEYEKDRFDRLLETYGGTATSHQLRLSGYSYFYYQEDDQDQVV
                     FGYQIKGNKCFVLGDPVGNPKMMRAATNQFMKQADCLGYQLAFYKISESYVVMLHELG
                     FHFTKVGEAGIVDLATEDLSRLSQQLEYQRLMKEGYHFTWYDQLPEELHQAVNEVSQK
                     WLQGAREKYFSVGRFDKSYVLSSGVGIVRKDQKVVGFITAQPMTKKQAGYDLLRVLPK
                     ESEELADYLLANLFIVYEKMGYSEANLGLAPLANVGETDFSFFQEKVMHIVYNYGNFF
                     YSFQSAYEKKLKYVDHWEGRYFAYRKGSNFGFATMQLLSLIGKEKGNVPSLAEEVLTE
                     L"
     gene            30097..30903
                     /gene="proC"
                     /locus_tag="ACG842_000104"
     CDS             30097..30903
                     /gene="proC"
                     /locus_tag="ACG842_000104"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HFQ0246676.1"
                     /translation="MKVGFIGTGNMAQAIIDGLLKKGTVSKEEVFVSSGHFENAQKFA
                     AETGVHACKSNQEVASQSDIVLLAVKPKIIEKVLEELREYSQKVLFISIASGKSLIDL
                     ETALEDPQASIVRVMPNVNVSVGAGVSAICKNEAATEEQLQQATMIFEAIGSVYPLAE
                     SDFSTFIGLAGSSPAFVYMLIDAMGRTGVLNGLSKQTATEIAAKAIMGSCQKFLSSDK
                     NPWELVDQVSSPGGTTVAGVVALEEAGFIPAIIKGINTTIEKDLEMLNKK"
     gene            complement(30982..32538)
                     /gene="rny"
                     /locus_tag="ACG842_000105"
     CDS             complement(30982..32538)
                     /gene="rny"
                     /locus_tag="ACG842_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HFQ0246677.1"
                     /translation="MVFNILLAIIGLIVGLGLGFMVAKSRHEKEIAGAQSSAAGIIDS
                     AKKEAETLKKEALLEAKEENQKYRSEVESELRESKLELKSQENRLIQREQTLNRKDDS
                     LEKRENSLESKEEALSSKQQLIEEREKDVEKMIEDQQKELEKIAGLSKEDARDVIMKS
                     TEEELNHELTLMVKESEQRAKEEADRKAKNLLSLAIQRCAADQVSETTVSVVTLPNDE
                     MKGRIIGREGRNIRTLETLTGIDLIIDDTPEAVVLSGFDPIRREIARMTLEKLIQDGR
                     IHPARIEEMVEKSRKEMDERIREYGEEAAFEVGAHTLHPDLIKIIGRLRFRTSYGQNV
                     LKHSVEVAKLAGILAAELGEDIQLAKRAGLLHDIGKALDHEIEGSHVEIGAELAAKYK
                     ENPVVINAIASHHGDVEATSVISVLVAAADALSAARPGARSESLENYIRRLRSLENIS
                     NSFAGVESSYAVQAGREVRVMVKPEEISDLDAVRLVRDIRKRIEEELDYPGHIKVIVI
                     RETRATDYAK"
     gene            complement(32949..33998)
                     /gene="recA"
                     /locus_tag="ACG842_000106"
     CDS             complement(32949..33998)
                     /gene="recA"
                     /locus_tag="ACG842_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HFQ0246678.1"
                     /translation="MADDRKAALDAALKKIEKSYGKGSIMKLGEKIDQQISTIPSGSL
                     ALDVALGVGGYPRGRIIEVYGPESSGKTTVALHAIAEVQKNGGTAAFIDAEHALDPQY
                     AQKLGVNIDELLLSQPDTGEQGLEIADALVSSGAVDIVVVDSVAALVPRAEIDGEMGD
                     SHVGLQARLMSQALRKLSGSINKTKTIAIFINQIREKVGVMFANPEITPGGRALKFYA
                     TIRLEVRRAEQLKQGTDIVGNRTKIKVVKNKVAPPFKIAEVDVMYGLGISQEGELLDM
                     AVEKDIVDKSGAWYSYKEDRIGQGRENAKIYMANHPEMMAEVSALVRAAYGIGEEVAV
                     PEDEKGQEELPLVEE"
     gene            complement(34107..35348)
                     /locus_tag="ACG842_000107"
     CDS             complement(34107..35348)
                     /locus_tag="ACG842_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HFQ0246679.1"
                     /translation="MKAEIIAVGTELLLGQVVNTNATFLSEQLADLGIEVYYQTVVGD
                     NQQRLEELIALAETRSELILLCGGLGPTEDDLTKEATAAHLGKSLIQNTEGYKKLLAY
                     FETTHRKMTKNNLQQSQIIEGGVPLPNRTGLALGTFYQTDTHAYILLPGPPNELKPMF
                     VEQVRPLLEERFPSEEKLISKVLRFYGIGESRLVTELKDLIETQINPTIAPYAKPNEV
                     TLRLTVKTNDVQAGNQALLALEEKIQERVGEYFYGYGDDNSLAKVVVELLKENKQTVT
                     AAESLTAGAFQAALGDIAGVSEVFPGGFVTYSLQTKAGFLEIDPELLAEYGTVSKECV
                     EQMAIQAREIARTDYSVAFTGVAGPDTLEGQSAGTVWIAISSKDGVVSEKYHFARDRA
                     AIRYNAVMQGFDLLRKQLLRK"
     gene            complement(35588..36166)
                     /gene="pgsA"
                     /locus_tag="ACG842_000108"
     CDS             complement(35588..36166)
                     /gene="pgsA"
                     /locus_tag="ACG842_000108"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HFQ0246680.1"
                     /translation="MNLPNKLTVLRICMIPIFILVVGLPLDWGQFNVGDTTLAVTQLV
                     GAVIFALASITDWLDGKIARARGLVTNFGKFADPLADKMLVMTAFIMLVDQGKAPGWV
                     VAIIVCRELAVTGLRLLLVEHGEVMAAAWPGKVKTATQMVAIILLFINNIPFSLIGIP
                     VDQIMLYACLIFTIYSGVDYFIKNSGVFKGSM"
     gene            complement(36375..37298)
                     /locus_tag="ACG842_000109"
     CDS             complement(36375..37298)
                     /locus_tag="ACG842_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RodZ domain-containing protein"
                     /protein_id="HFQ0246681.1"
                     /translation="MLRRIKQTGEDERVAFETIGEKLRQARLNKKISLDELQQITKIQ
                     KRYLEAIDNDDFDQLPGKFYVRAFIRQYAEAVGEDGDHLVDVFDGKSKLTGSVPERPQ
                     PETVSTSRKAMHQEESHPSRFWTSLPVILLGLVALAIVVVVGYMTWQDRQADPIIGDT
                     ASSLKVEGSVEESSSSEQSTQTKESSTVESTTKSSEEKKMAIATGEDSGSAIAVNITD
                     ATKPVTLEFTAVNRVWLGVLVDNAYVYQGTLAANETQSTVLPETATNATITIGAASNA
                     TIKANGESVPVNPGENNQSPKNVNLTLQYAE"
     gene            complement(38037..39323)
                     /locus_tag="ACG842_000110"
     CDS             complement(38037..39323)
                     /locus_tag="ACG842_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HFQ0246682.1"
                     /translation="MNKTEYEQINETLYHEVLPNGLTVYLLPKNDYHKTYGLFSTNYG
                     SIDNEFIPYGEKEKVKVPDGIAHFLEHKLFEKEDGDVFQLFGKQGASANAFTSFTKTS
                     YLFSTTDQVEKNLTTLIDFVQAPYFTEETVNKEKGIIGQEIQMYEDDPNWRMFFGILN
                     NLYPTHPLHIDIAGTVESIDKITAQDLYTCYRTFYQPSNMVLFVVGKMEPEKLMKLIR
                     ENQEAKNFPPKQEIVRYFPENTKEIIKQSALEAAITRDKFVLGIKGLDTLPQEGTELL
                     RYKTAINLLFQMILGNTSRNYLAMYNQGIIDDSFGFEFSLDREFHFADFSGDTDEPEK
                     AAEKVKEIILGFADDPEVSETNLDLLKKKMLGQYFQSLNSIEYIANQFTQSLFGDRTL
                     FDLPEIIDSIQMKDVLAAGEAFISEEAFSEFYMRPK"
     gene            complement(39316..40584)
                     /locus_tag="ACG842_000111"
     CDS             complement(39316..40584)
                     /locus_tag="ACG842_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HFQ0246683.1"
                     /translation="MVYQLAEGVNLHVLPTKQYKTIRIFIRFTARLQQEVITKRSLLS
                     SMLETNSLNYSDQTKLSAQLAELYGASFGLSVRKKGNLHWLNAGISFVNGEYVNDPNL
                     FSQAVDFLKEVLFYPNIKNHQFDQLTFDLEKNNLRLYLESLKEDKQTFASYALQELYF
                     ENSPEQKIPGLGVVEDLDKITASSLASYYQEMMANDQIDIFVVGDVDPDKAAEAVGQL
                     PFESRETVYPDLFYTQPQVNIVKERQVREPIVQAKLNLAYQTNVYYDEPERFALMVFN
                     GLFGGFPHSKLFMNVREKESLAYYASSSVDTFRGFMSVQTGIDEKNRNQVLHLIHEQL
                     ESLRNGEITELELSQTKAMLRNQYLLSLDSPQAAIEASFLDSWLPETKLSDEEWLKRM
                     ESVTIKEIQQVAEQIELQAIFFLAGGNANE"
     gene            40836..41297
                     /gene="mscL"
                     /locus_tag="ACG842_000112"
     CDS             40836..41297
                     /gene="mscL"
                     /locus_tag="ACG842_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HFQ0246684.1"
                     /translation="MIKEFKEFIMRGDVLDLAVGVVIGSAFTSIVNQVVQGLITPLIG
                     WFVAMITGTSDLEGALSILDWSPTKGVTFAFGNVISAIITFLITGFVLFLVVKAANRA
                     KNLRSKEEQVEVEETPTAEDYLSDIRDLLAKQVAEDNTHPVEQTNLSDTSK"
     gene            complement(41356..41802)
                     /locus_tag="ACG842_000113"
     CDS             complement(41356..41802)
                     /locus_tag="ACG842_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0246685.1"
                     /translation="MEYQVKKFEELSTAEFYAIVKERIAVFVVEQQCPYQEVDDIDGE
                     AWHTFFQDTDGKILAYTRVYEEAESIHFGRVLVHQDNRKDGLGRKIVQETINMIQKNF
                     PEKPIVIGAQEYLQAFYESFGFKAISDVYLEDDIPHIDMKKEQVAS"
     gene            complement(41895..42140)
                     /locus_tag="ACG842_000114"
     CDS             complement(41895..42140)
                     /locus_tag="ACG842_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Veg family protein"
                     /protein_id="HFQ0246686.1"
                     /translation="MPTTLASIKKDLEGRIGSPIMLVAQTGRKRQTERRGVLTETYPS
                     VFVVDLDPEENSFERVSYSYSDVLTRTVEIEFLTEAV"
     gene            complement(42255..42833)
                     /locus_tag="ACG842_000115"
     CDS             complement(42255..42833)
                     /locus_tag="ACG842_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HFQ0246687.1"
                     /translation="MSEELLRAARNGDEEAFAALYQKYFPLLLRLKNTYYLRNYVSED
                     WEQEARIALYQALRTYEKSKGVSFGSYYKSVISNQIYSLLRKQNALKRRDQKDEVSID
                     QKIETEGPDFLAEITLQEPLAIQHLLLKEAIENCEIQFSKIEAKIFHYYMQGVDFVDM
                     AECLAMDHKQVMSAYGRVKLKIKKHLKFYNEE"
     gene            complement(42910..43440)
                     /locus_tag="ACG842_000116"
     CDS             complement(42910..43440)
                     /locus_tag="ACG842_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HFQ0246688.1"
                     /translation="MKKQLLIVDGYNMIGSWPELVQLKKQDKMADAREALLHRLSNYA
                     KYEGMEVIVVFDAQLVPGIQQNYKKYQLDVVFTKEDETADSYIERIAGQLNDRLTQVT
                     VATSDLAEQWMIFSQGALRTSAYELYKTIKKTEKTIAQENKDMHFIDFRRNSPWNEEQ
                     LKKLSQKLDDLMRKNK"
     gene            complement(43444..44304)
                     /gene="rlmB"
                     /locus_tag="ACG842_000117"
     CDS             complement(43444..44304)
                     /gene="rlmB"
                     /locus_tag="ACG842_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HFQ0246689.1"
                     /translation="MGKERTNKHQSKRGFEDKKQNRYSNKKQTGRNQSVSDQNEEALE
                     DNFVFGHHAAVEALQQGRGNKLFLQEDSRGEKVEELKEIAREQAVPVKWVPKQKLETL
                     SDHGVHQGVVLAITPYEYLSLDQLIERTKEKKEDPFFLILDSIEDPHNFGSILRTADA
                     SGVDGVIIPKHRAVGITPIVTKTSTGAVEHVPVARVTNLVQAVQQLKETGFWVFGTDM
                     TGTNFQKWNAKGSIALVIGNEGKGMSPGLKKEMDELLTIPMTGHVQSLNASVAAGLLM
                     YQAFSQRQGE"
     gene            complement(44297..44692)
                     /locus_tag="ACG842_000118"
     CDS             complement(44297..44692)
                     /locus_tag="ACG842_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mini-ribonuclease 3"
                     /protein_id="HFQ0246690.1"
                     /translation="MRDYTQLNGLALAYVGDAIYEIYVRDHLIDQGETKPNKLHRAAT
                     NYVSANAQAFLIQQMLEEHLLTEEEELYYRRGRNAKSHTSAKNADVTTYRVATGFEAL
                     MGYLHLTKQTERMEELIRWCIQKVGEKNG"
     gene            complement(44689..46101)
                     /gene="cysS"
                     /locus_tag="ACG842_000119"
     CDS             complement(44689..46101)
                     /gene="cysS"
                     /locus_tag="ACG842_000119"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HFQ0246691.1"
                     /translation="MIKIYNTLTRKKEEFIPIQKGHVGMYVCGPTVYNYIHIGNARST
                     IAFDTIRRYFEYRGYKVDFVSNFTDVDDKIIRTANELGITAPEVADRFIRAFEEDTKA
                     LNVEPATLHPRVMDHMPDILAFIQVLVEKGYAYESSGDVYYRTRKFENYGKLSDQSID
                     ELEVGASQRTGEEQQQKEDPLDFALWKQAKPGEISWDSPWGKGRPGWHIECSVMATKH
                     LGETIDIHGGGQDLEFPHHENEIAQSEAKTGKKFANYWMHNGYVTIGEDDEKMSKSLG
                     NFITVHEMIKNVDPQVLRFFMATTQYRRPIRYSETTMKEAATNLQRLKNTFDNLKFRQ
                     ETASENLADDADKIALLENLEQRFIEEMDDDFNAANGITVVYELAKWLNTYLDQPKVS
                     AAVLAQGEALFSQWLSIFGILFTAEELLDEEIEQLIEERNQARSNRDFVRSDEIRDLL
                     KEKGILLEDTPQGTRWRREA"
     gene            complement(46098..46634)
                     /gene="epsC"
                     /locus_tag="ACG842_000120"
     CDS             complement(46098..46634)
                     /gene="epsC"
                     /locus_tag="ACG842_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase EpsC"
                     /protein_id="HFQ0246692.1"
                     /translation="MSWFTRAIDAAKRNDPAARSTAEIVLTYPGFHALFWHRFSHFLY
                     QHRLFLLAKINAQFWRFLTGVEIHPGATIGTGVFIDHGMGIVIGETAEIEDDVILFHG
                     VTLGGTGKETGKRHPTVKQGAMLSANAQILGPVTIGKNAKIGAGAVVLKDVPDDATAV
                     GVPAKVVRIKGEKVRRSE"
     misc_binding    complement(46720..46959)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(46956..48416)
                     /gene="gltX"
                     /locus_tag="ACG842_000121"
     CDS             complement(46956..48416)
                     /gene="gltX"
                     /locus_tag="ACG842_000121"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HFQ0246693.1"
                     /translation="MTKVRVRYAPSPTGHLHIGNARTALFNYLFARHNDGDFIIRIED
                     TDQKRNIEDGEKSQLENLAWLGMNWDESPENPGEYGPYRQSERKEIYQPLIDQLLASN
                     RAYKCYCTEEELEAEREAQRARGEMPHYAGTCANLTPEEQAEKEAQGLESVVRFRVPR
                     NTEYAFTDMVKGAISFESDNIGGDFVIQKRDGMPTYNFAVAVDDHLMKITHVLRGDDH
                     IANTPKQLMIYEAFGWTPPTFGHMTLIINSETGKKLSKRDESILQFIEQYRELGYLPD
                     AMFNFIALLGWSPVGEDEIFEHQELIKMFDPKRLSKSPAAFDAKKLQWVNNHYIKAMD
                     LDAFTDMCLPYLIADGRVAENPDEKTIEWVKKIVSLYQPQMSYAAEIVDLSTLFFNEH
                     PVLNEAAKEVLAGETVPTVLHAFKAQLEQMEVVDAPTVKAAIKAVQKETGVKGKNLFM
                     PIRVAVSGQMHGPELPETIELLGREKSLAHLNQVLN"
     gene            complement(48586..49743)
                     /locus_tag="ACG842_000122"
     CDS             complement(48586..49743)
                     /locus_tag="ACG842_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN/TRAM domain-containing protein"
                     /protein_id="HFQ0246694.1"
                     /translation="MQKRVITLLMIIVGASLGISFLPSAWQAIGQQSNGWLNNTFTNS
                     LLGALIFFLLSLLLAKHISAIIKQVEQKLSEVSLTYLLFGAIGAIIGLTLGVVISTPL
                     YNMEIPFVNSVLPILLMIILGYLGLRMGTTRIEEWKKVFGSRSKKIEQETASQVESQL
                     LERKSGENFHKYKILDTSVIIDGRIYDITKTGFLEGTLMIPNFVLYELQYIADSGDSL
                     KRVRGRRGLDILNALQKEEGISVEMYDGDFEDISEVDSKLIKLAKLLDGVIVTNDYNL
                     NKVSEFQNVPVLNINALANAVKPVVIPGETMNVLVVKAGTERQQGVAYLDDGTMVVVE
                     DGQHYMNERIEVVVTSALQTAAGRMIFAKPAHAGRGIDNKKETEKKTKQSK"
     gene            complement(49777..51147)
                     /gene="radA"
                     /locus_tag="ACG842_000123"
     CDS             complement(49777..51147)
                     /gene="radA"
                     /locus_tag="ACG842_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HFQ0246695.1"
                     /translation="MAKKSKVQFVCQNCGYVSPKFLGRCPNCGKWNTMVEEIEQDTTD
                     RRTRTSLTGEKAKPTKIADVVPKKEPRIKTKLEELNRVLGGGVVPGSMVLIGGDPGIG
                     KSTLLLQVSQQLAAIGGKVLYVSGEESAEQIKLRAERLGSINTEFYLYAETDMNEISR
                     AIEHISPDYVIIDSIQTMTQPDITSVAGSVSQVRETTAELLKTAKTNGIAIFIVGHVT
                     KEGSIAGPRMLEHMVDTVLYFEGEQHHSFRILRAVKNRFGSTNEIGIFEMHEHGLEEV
                     ANPSQIFLEERLDGATGSAIVVAMEGTRPILVEIQALVTPTMFGNAKRTTTGLDFNRV
                     SLIMAVLEKRAGLLLQNQDAYLKAAGGVKLNEPAIDLAIAVSIASSYKEKGTQPTECF
                     IGEIGLTGEIRRVSHIEQRVKEVQKLGFTKVYLPKNNLGNWEAPKGIEIVGVATLAET
                     LKRVFR"
     gene            complement(51210..51737)
                     /locus_tag="ACG842_000124"
     CDS             complement(51210..51737)
                     /locus_tag="ACG842_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HFQ0246696.1"
                     /translation="MRKRGFEVISKYKDQNINLPQRATHHAAGYDFEAAEEVVIPSIW
                     KAGWKKGLETVADGLKKDVSENEKQLKPTLVPTGIKAYMGEDEFLQLANRSSNPLKRF
                     LVLTNGVGVIDSDYYNNEANEGHIMFQFTNFGFKDVVIKKGERIGQGIFLPFLKADQD
                     QTTAQRQGGFGSSSK"
     gene            51967..52773
                     /gene="proB"
                     /locus_tag="ACG842_000125"
     CDS             51967..52773
                     /gene="proB"
                     /locus_tag="ACG842_000125"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HFQ0246697.1"
                     /translation="MRKEIKNAKRIIVKVGTSTLIYPNGNINLAAIDQLAFVLSDLRN
                     RGKEVILVSSGAVGVGMHKLHLSERPTLIPEQQAAAAVGQAELMNIYNQRFSVYNQQT
                     AQILVTRDVVDYPESRKNVRNTLEQLLIMDTIPIVNENDTVAVDELDHLTKFGDNDQL
                     SAIMAKLVAADLLIVLSDIDGFYSDNPTTNPEAQMYRYIDKIDEKILAQAGGAGSTFG
                     TGGMQSKLKAASRIFEANSAMILANGKNPAIIFDILAGKEVGTLFKEDLK"
     gene            52770..54017
                     /locus_tag="ACG842_000126"
     CDS             52770..54017
                     /locus_tag="ACG842_000126"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HFQ0246698.1"
                     /translation="MTDLMQLGQQAKDSAQQLALMPTNRKNELLVQMAQTIKKNQQAI
                     IEANHKDLEKAVENNISETMQDRLQLTPERINAMATEIEKIASLDDPIGKVDEMWTNT
                     DGLRIGKKRVPLGVIGIIYESRPNVTTDAASLAFKSGNAVILRGGKEAFFSNQLLVQL
                     LQQVLVSAGESPYAIQFVDDTSHETAGQLMRLTEYLDVLIPRGGAKLIQRVKEQATVP
                     IIETGTGNNHVYIDKEAQLSMAVNIIVNAKASRPSVCNAAETLLIHSEIAPFFLPAIE
                     KELVEHGVSLRADARALEYLETAALAEDTDWDTEYLDYILAVKVVDSLDEAIAHINRH
                     NTKHSETIVTDSYAASQRFLNEVDAAAVYVNASTRFTDGSVFGFGAEIGISTQKLHAR
                     GPMGLNELTSTKYIIYGDGQIRE"
     gene            54093..54437
                     /locus_tag="ACG842_000127"
     CDS             54093..54437
                     /locus_tag="ACG842_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0246699.1"
                     /translation="MFEILMIFFIYLISLNIAAFLGVGILSLFFQFKKRSIGSQREKW
                     SQYFDKIGQKGLVTRLHISYMVALCLLATINYYSFFDHSIAYTITLLIAGIFHLSYKY
                     QLNKNHLNRTFR"
     gene            complement(54554..54880)
                     /locus_tag="ACG842_000128"
     CDS             complement(54554..54880)
                     /locus_tag="ACG842_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0246700.1"
                     /translation="MLEEYFQEVGLTLVELPIEFSDDRLIYRGYIKELPIINAEGETR
                     KAMQQKIAGMYQVYREQLLAESAEEEKEKTMNLSMDELLRYYDGETFDGFSLLNEEET
                     DLPGSW"
     gene            complement(55495..56697)
                     /locus_tag="ACG842_000129"
     CDS             complement(55495..56697)
                     /locus_tag="ACG842_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFQ0246701.1"
                     /translation="MEILENQIDIQEQTNEETKGKSSALVVAATCMIAFMGVGLVDPI
                     LKTIAAQLNATPAETTLLFTSYMLVTGVVMLFTGFLSSRIGLKKTLMTGLFIIVVFAG
                     LGGLSGNIGMLIGLRAGWGVGNALFVSTALATIVSILVGNTEKAIMMYEAALGCGMAV
                     GPLVGGVLGSGSWRYPFFGVAFLMFCGFIALAVLLPETEKPKRKVGLWEGVKALSNHK
                     LRSVGLIALLYNFGFFTLLAYAPFLLIGYSELEVGFVFFGWGVLLAIASIFVAPALER
                     KFTTYRTILAAFVLFIICLLLLGAGTSMPVLVPISIVLAGFFQGIINTLLTTIAMEIP
                     GLQRNVASSSYSFVRFFGGALAPFIAGVIGEHIGESYAFYFAGAIVLISVCCIIYHRN
                     YFATNEEE"
     gene            56814..57329
                     /locus_tag="ACG842_000130"
     CDS             56814..57329
                     /locus_tag="ACG842_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFQ0246702.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            complement(57528..57974)
                     /locus_tag="ACG842_000131"
     CDS             complement(57528..57974)
                     /locus_tag="ACG842_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HFQ0246703.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(58021..59499)
                     /gene="msr(C)"
                     /locus_tag="ACG842_000132"
     CDS             complement(58021..59499)
                     /gene="msr(C)"
                     /locus_tag="ACG842_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(C)"
                     /protein_id="HFQ0246704.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRLDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            complement(59853..60482)
                     /locus_tag="ACG842_000133"
     CDS             complement(59853..60482)
                     /locus_tag="ACG842_000133"
                     /EC_number="1.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016657 - obsolete oxidoreductase
                     activity, acting on NAD(P)H, nitrogenous group as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HFQ0246705.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMKPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITMFE"
     gene            complement(60466..60930)
                     /locus_tag="ACG842_000134"
     CDS             complement(60466..60930)
                     /locus_tag="ACG842_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFQ0246706.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIKH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            complement(61417..62685)
                     /locus_tag="ACG842_000135"
     CDS             complement(61417..62685)
                     /locus_tag="ACG842_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HFQ0246707.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            complement(62916..63653)
                     /locus_tag="ACG842_000136"
     CDS             complement(62916..63653)
                     /locus_tag="ACG842_000136"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFQ0246708.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            complement(63720..64289)
                     /locus_tag="ACG842_000137"
     CDS             complement(63720..64289)
                     /locus_tag="ACG842_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DapH/DapD/GlmU-related protein"
                     /protein_id="HFQ0246709.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLSVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            64417..65289
                     /locus_tag="ACG842_000138"
     CDS             64417..65289
                     /locus_tag="ACG842_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFQ0246710.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKVFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
     gene            65631..66503
                     /locus_tag="ACG842_000139"
     CDS             65631..66503
                     /locus_tag="ACG842_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFQ0246711.1"
                     /translation="MKIKKIFNQNAVLVDDGGQEKVAIGKGIGFDRKRNDLIFDRDVE
                     RIFVMEPEGQIKLQNLLNQIDERFLFAAETIIDHAETVLMEKLNEHVLIALTDHIAFS
                     AENINNGIVIRNKLLREIEVLYSDEFSIAQWAVEYLTKELGIPYTYDEAGYIAIHIHS
                     GRSGRNNNHRSIREVTIVSDIIHLVESELETDIHSEPFSLNYSRLVNHLRLLLQRTHA
                     QQYAVLDTEIVEMVKRKYPESYKISKKIRVLLTKEYQLAITKEELGYLAIHIERLRSA
                     IVQTNVNNHEEEKN"
     gene            66500..68458
                     /gene="nagE"
                     /locus_tag="ACG842_000140"
     CDS             66500..68458
                     /gene="nagE"
                     /locus_tag="ACG842_000140"
                     /EC_number="2.7.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015572 - N-acetylglucosamine
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-specific PTS transporter
                     subunit IIBC"
                     /protein_id="HFQ0246712.1"
                     /translation="MKAYLQRMGRSLQLPVAVLPAAALLVGIGNWWASYSNDIVAHFL
                     QAGGNAVLGQLPLLFAVGLALGMSVDKSGAAALAGLVAFEVPVNVLKTDSVATLLNIK
                     PESVDPSFAQISNVFIGIIAGLIAAALYNRFHEAKLPMALSFFSGKRLVPILAAFVML
                     LISAVLLLVWPPVYSALVSFAKFILSLGWGGAGLYGFFNRLLIPTGLHQAMNSVFWFD
                     VAGINDIGKFLASEGTKGVTGMYQAGFFPIMMFGLPAGAYAIYRNARPENKAKTASLM
                     LAAAFASFFTGVTEPLEFSFMFVAWPLYVLHAIFTGISLAVSAFFHWTAGFAFSAGFV
                     DYFLSLKNPIANQPLMLIVQGLVTAVIYYFGFNFAIKKFHLMTPGREEADLSDDDTAT
                     TNSDNKYAAQASKIYAALGGADNVTSIDNCTTRLRLQVKDTGLIDQNKIKATGVPGMK
                     IIDGKNAQVIVGTEVQFVADEMAKLHGGAATRPAQTNTVVKTETFAGGTIEQDIYAIA
                     NGKLIPITEVSDDVFAEKMMGDGYAVLPENGEIFSPIAGTITNIFPTKHAVGIQTDAG
                     IEVLLHMGINTVDLKGEPFTLYVKEGQKVARGQLIALVDLAAIQSAGKNTDMVVVFTN
                     GDKVQSLEIEPARDVKANDKIGSVSNKA"
     gene            complement(68790..69641)
                     /locus_tag="ACG842_000141"
     CDS             complement(68790..69641)
                     /locus_tag="ACG842_000141"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFQ0246713.1"
                     /translation="MHYLVTGATGGFGGYALEFLKKMVPQNEIYVLVRSEEKGEALKE
                     AGFNIRIGDYDDLDSMKEALKGIDRLLFVSGVPGNRQAEHKNVVKAAQAAGVSYIAYT
                     SFADADHSISVLAPDHQFTEKMIKDSGISHTFLRNNWYVENELPIIGQALKTGQFVYA
                     AGNGKTGWVLKREYAEAAAKVLVESDSPEILELSGNLTDYEELAKALERATGKKLEII
                     EASDAAFVENLKEAGFPQEAADMFLAIQHDIKKDQLAISSDDLEKVLGKPLTSLEAAL
                     KEILDQE"
     gene            69764..70231
                     /locus_tag="ACG842_000142"
     CDS             69764..70231
                     /locus_tag="ACG842_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HFQ0246714.1"
                     /translation="MKYSLQFSDAIHVLAYIEMFQDTRSLSSEMIASSIETNAANVRK
                     IMSQLKKSGLIFTKTGKPQPSLAKSPDQISLLDVYRSIEGNTQLIQVDPKTNPNCVVG
                     ANIQEALEESYARLQKKAEEEMAKITLDSLIHKIAVLEKEKRPENTGLVERFL"
     gene            complement(70494..70976)
                     /locus_tag="ACG842_000143"
     CDS             complement(70494..70976)
                     /locus_tag="ACG842_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFQ0246715.1"
                     /translation="MAKYRIEEKPDFTVTGIGVELKSSNTDFVGLAAEKQAFEQKVIG
                     NGTIDALKQAAENHYFFTVNEPYNGKMMYYVGVQTSQDVPNTTRIIPFPKGTYLVVIA
                     LAESPEELRNDLTNTAFGHVLREVEDYAYVGGPNAVVEMGVTNGKYMGEIWIPVVNKP
                     "
     gene            complement(71076..71939)
                     /locus_tag="ACG842_000144"
     CDS             complement(71076..71939)
                     /locus_tag="ACG842_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFQ0246716.1"
                     /translation="MLKDLNQLIDYIEEHLTEGLSYKIIAENTGISEYQLKRIFTFIA
                     GMSLMDYIKNRRLALANRDLLDGSRVTETAFRYGYETVEGFSRAFRDWSGYLPSEISR
                     YGIQKSFPRLTFFIDVRGGKSMEFRIEKKEQFYVAGVAKKVPVKFEGVNQEIQKLAQT
                     ITETQREEMHKLGDLYPNQVINVSYGFDGARLEEKGSLMHMIGFATTKENPYADLEML
                     AIEEHTWAIFPNKGPFPQTLQDTWGKIYAEWLPSSDYELVEAPEISFTKWGEDFSNVY
                     SEIWIAVKAKK"
     gene            72537..73412
                     /locus_tag="ACG842_000145"
     CDS             72537..73412
                     /locus_tag="ACG842_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger family protein"
                     /protein_id="HFQ0246717.1"
                     /translation="MNNWLEYFPENVLERGYSYHLHGFVRHLNYTSKYLSATVSGTED
                     YKVVITWDEKTNMTCDCLYAIEGKKCKHMAAVLFAYEERPIKKSNYSLSELSSLVSSA
                     SSSLVRELLTEILIEHPQFIERFKVKMPFHAINYSDKLTTIIHKYDHIIKKNKNRKTA
                     KFIMEMRKFIQEAVESLIQQNAYLPAFELINEVIATLETFYWEPEDERTLLLIEDCYY
                     LWKELLAEAPHAEKRQMFSWFVCQVDHTDASYSKRYSIKILKEDFREKEFSNQKKKID
                     KKTKETVKKDDFNEK"
     gene            complement(73557..73709)
                     /locus_tag="ACG842_000146"
                     /pseudo
     CDS             complement(73557..73709)
                     /locus_tag="ACG842_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874852.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="xylulose kinase"
BASE COUNT        20783 a        15759 c        13182 g        24043 t
ORIGIN      
        1 ctgttgttga ttcttctgtt gctgaagatt caggcgctgt tgattcttct gttgaagatt
       61 caggtgttgt tgattcttct gttgctgaag attgtgtagc tgatgatgat tcttcagtca
      121 ttgaagattg tgtagctgat gattcttctg ttgctgagct ttgtgcagta gatgaagctt
      181 gagtagcttg tgcttcagcc tctgcttgtt cgcgtgcttc tttttcagct ttttcttgtg
      241 cagcttgttg acgagcttgt tctgctagac gttgttgttc acgaatacga gcttgctctg
      301 cttcagcttc tgctttttga cggttcaagt cagctttctt gtcttcagca gttgcttgtt
      361 cagcagctaa tgaagttttc agtacgttca aatcagcttg tttagataaa agatcgcctt
      421 tttgagattc caaagcagct tggttttcag ctaattcttt tagtttagca tcgttttcag
      481 cttttttgtc ttctacagct tttttatctt gtttttgttg ttccatcaag tcgttgtttg
      541 cttttaccat cgttgtcatt gcttgaacgc gtccaatagc atctgctaat gaatcagcat
      601 tcaatacagc atcgatgtag tttgagcttg tgttgcttac ttgtgcttcg cgtgcttgtt
      661 tttggattgt atcttcacgt ttttcaatac gttcttgtaa atctgcaatg tcttttacta
      721 attgtgcaga ttcttgacgc aatgtatctt gttttgctaa taggtcttgt gcttgtgtgt
      781 tgatttcaga aacttggctt tctaaggcat cgatttgtga ttgtgcatct gcttgttgat
      841 ttttcaagtc tgcaatcttt tgatcttgct gttgtatctg agaatcaaaa tcgtccgccg
      901 cggcagcgat cggtgatgct acggcagtca aggtcattga acataccatt actgctgata
      961 ttaaactctt tttcactctt cattcctccg actggcttaa tttaataaat atctaaatat
     1021 atttaagtct ttcttaacga tataaaaatt gtaccatagc agtgtgtcaa cgatataata
     1081 gttatgttac aaaaatgttt caaaacaact tttaaaaatg acaaaactgt tcgtttcaga
     1141 aaatgatcat gaaaaagccg cgttaagtct agcatttctt aagataacaa cgattctttt
     1201 tcgctttcga cctaaaaata aaaaaagaaa ttattcatca ctgaataatt tcttaaaggg
     1261 aaaaatgaat aatacaaata taatcatgtt caacagcagt gtaggtccta aaaaccttgt
     1321 aataaaatac gtgttattta ctactgctaa tttaaaaatc aactggacca ccatcgtaaa
     1381 caattcataa cccgtgacaa agatgatcat actgaaaaat tcagtaaaga tgttgacatg
     1441 gataacgtct ttcattacat acatcaacca tacaattaaa gggagagcta ctgcatagat
     1501 accgataacg ccaatatagt aagcgtcata gatagccccg agaacaatcg tggtaatcag
     1561 taaataacgt ttttcaaacg ccatactgca acataataat gccaatatca gaaagtgggc
     1621 atttgacata taggtgcctt tcgaccactc ccctagcatt cttgttaaat gaccatctat
     1681 caacatcaaa aagaacaaaa cgattggcag atagtacttc attgtttctt tcttcaacat
     1741 ggactaatcc tccgctagcc gttggacaat cgtcacaact gacacgtcat acatttctgc
     1801 ataaggtttc acataaactt cccgatccag cccataacta tctggtttcg ttttaataac
     1861 cgtaccgatc ggcaagttag ctggagaatt ccctccaaga ccggatgtct ggacaacatc
     1921 cccttctttg atatccgtat caccagttaa ttgggtcacc actaaggcat ggagcttttc
     1981 atcatagttt ttaagcaaac caaacgcttc gccattagcc gaagataccc gtactggaaa
     2041 atgattggag ctttcattag aggatgacag taattcgatt ttagacgaag ccgtattgac
     2101 ctcgatcact cggccaatca atcctttttg cgacatgact gccatatttg cttcgatacc
     2161 gtctttagaa cccttgtcta cgatcagcat gtcctgccaa ctgtcaggcg aacgagtaat
     2221 gacatttgcc gttacttttt cgtaactagt cagcgtctga ttcaactcaa gttctttttt
     2281 caacgcttcg atttctctct cgtaattctt gttttgctgt gaaacgtcat catagctatc
     2341 gattttttct tttagccgct cattttcggc ataagttgaa aaaagattat ggacagaatc
     2401 tactccattt cgtacccacc gtactggggc agagattgtt ttatcaacca ttgcaacact
     2461 gtcattaacg atggattgga agaaattggg ctttccttca ttcgctcgct gcgcagcggt
     2521 cacacttagt atcgtcacca cgatgataac aataaccaag gtaataataa tatttttatt
     2581 aggattgaac ttcttcacaa agacacctcg aattaattag actcttgtgt tcaattttat
     2641 cactaaatgc cgtgaaacaa aaggtaagtt gctcctctta agcttttttt tagcaaataa
     2701 tagattctgt catttttagc ctgaaagaca aattttttta tttgtttatg gatcaatctt
     2761 tgctttgaat aactgcaatc ataccatctg aaaaagaaaa ttctgcggta tctgaaagaa
     2821 attcgttact tgcataagat gtcggatatt ctacttcttt attttccaat acatacttgc
     2881 tgccttttaa tgtaagattc gagacgggag tcaagcagca ataagctaaa tacttcatat
     2941 ctggtatctg tcttactata tgctctccag gcaaataata agacaaataa ttttgacaat
     3001 ctctgataat aagctgatgg gcaaagggtt tgaagcgagg ttccatacct aaccagagat
     3061 tggctaaaag atgatccaat cttccgcccg tagctccaat caaagtgact tccgcttgcg
     3121 ggtaacgttc aaatgttttt aagataccta actgtgtatc tgtatcgtct ttttctgcag
     3181 gggaacgggt atactctttg gcttttgcaa atacttcctt ctgctcttcg ttcgacaatg
     3241 aatcaaaatc acccactgcc agatctactg ggtagcctgc ttgaagcagg tacagcgcac
     3301 cacggtctat cccgacatag gcatcgtatt tggacagcag agactgcggc cagttttgcg
     3361 gattacctcc agcagctaat aaaattttca tttagtctaa tgcctctttt aatcgttcga
     3421 tttgtccgac tggattttcg gctccataga tatatgatcc tgcaacgaag acatccgccc
     3481 ctgcatcttt acagattttt gctgtttctg gaacgattcc gccatctact tcgattgcat
     3541 aagtccaatt gttctctcta cgtagatcag cgagttccgt aattttttct actgtttctt
     3601 caataaagct ttgtccgccg aacccaggat tgacagtcat gactagtact tgatcagcca
     3661 aaggcaaaac atgtttgatc atggagactg gtgttcctgg attgataact actccagctt
     3721 taactcctaa gctttttgtc atttgaagtg cccgatgaat atgaggagtt gcttcgacgt
     3781 ggacagtaat gatatctgct ccagcttttg caaatgctgg aatgtagttt tctggatcaa
     3841 cgatcatcag atggacgtct aacggtaatt tagtaacagg acggattgcc tgaacaatat
     3901 ttggccctaa tgtgatattt gggacaaatc ggccatccat tacatctacg tggatgtaat
     3961 ctgcgcctcc tttttccact aactcaatgt ctctttgtaa attggcaaaa tctgcactca
     4021 aaatggatgg tgctatcttc attcttgttt cctcctattt tcgtttacct tctttttttg
     4081 catacattgg ttttcttttt tcgatttctt gtaagaattg caaataattg tcgtaacgtg
     4141 tctgtgcgat accacctgtt tctactctat gttttacttc gcagccaggt tctttagcat
     4201 gcatgcactc tctaaatttg caatggacag aagcttctac aaattcagga aattgtctcg
     4261 gcagttctcc tgcttcaatt gtcaaaaagt caatcgagct aaatcctggt gtatctgcta
     4321 ctaacccgcc atagatagga atcagttcca catgacgtgt tgtatgccgt cctcttccaa
     4381 gtgcatcaga aatctctcct gttgctaaat tcagttccgg tgatatttga ttcagtagcg
     4441 tcgattttcc tgctccagac tgacccataa agaccgatag ccgttctggg aagtaacgga
     4501 tcagttctgt cgtatcaccc gtatgattgg gtacaatcac cggatagcct attttagtat
     4561 aggtttgctt gatctcttcg attttttctg ggtcgtctga caagtcggtc tttgtcaaaa
     4621 aaataatcgg ttcgatcgac tcgtattcca aggtaactag aaagcggtca agtagattat
     4681 aagaaaatgc aggctctacg atacttgtga caatgattcc aagatcaaca tttgtgactg
     4741 gtgggcgaac taattgattt tttctcggca agatttccag aagatagccg tctgtttgat
     4801 tttcactctc aaagatcacc tgatctccga caagcggcgt gattttccga ttccggaaat
     4861 ttcctcttcc tcttgtttga taggtctctc cgtttgcgta tacatagtaa aaaccgctga
     4921 ttgcttttct gatttgtcct ttgataagtg ccacctccgt ttttgtttat tttaccatac
     4981 gaagaggaaa gtaggtgtta ttccatgcaa acttctcaaa gtatatggct tgaaaattga
     5041 tcaaaagaga tagaaacaca aaaaagagaa tgcaacaaat caatgtcgca ttcccttctg
     5101 atatatttta ttcagcactt attttgaact ttctgaggtg ctagctgatg aacttgtgga
     5161 gctggaagat tcttcttttg aagagttgct tgtgctactg gagctacttg agctgctaga
     5221 actgtctgaa gagtcttttt cagaactaga agacgctgga tctggtcctt tagagtaaat
     5281 tacgttcaca accattcctt tggtaattgt cgttcctgcc cctggtactg tacgaatcac
     5341 tttatcttta tccaccgtgt ttgaatattc ggaagtctca tgcccgatat attctgcacc
     5401 gacactagct aaataacttt gtgcttcgct ttttgtatag ccggagatat cattcaattt
     5461 aggctctgtt ccttggctta cgacaaaatt gatgtaagtt tcttctgcta caacttcacc
     5521 cgaggctgga tcctgactga tgatcgaacc tgatggtaca gtgtcgctat agtcataagt
     5581 agcactaata ttattgatat tgaatccttg tgtaacaagc caattacgaa cagtttcgta
     5641 gttttctcct acatgactac tcaaagtcac tgtttctggt cctttgctga ctgtgaaagt
     5701 aatcgtgtct gttttaggat cgacttctgt atcttctgat ggagattgcg aaatgatcat
     5761 tccgctttct acctctgaac tgaattcctc ttctttttga atcaagtcag cagaaaaccc
     5821 taattctttc aaacttgctt cggcatcttc ataactttta ccactgtaat catctaattt
     5881 gatttttttc gtgccgctac taatatacag agtgacttcg cgtttttgct tgactgtcgt
     5941 tcccgctgcc gggtctgtct tcactacatt accttcttcg attttgtcat ctgcaatttc
     6001 ttctgttttt gttgctactt ctaatttcgc ttttgcgagt gtttctctag cagaagcttc
     6061 agaaagatca gaaacatcag gaacaacaac ttctcctcgc ccgccagaag caaagaatac
     6121 agctgttccg attcctaaaa cagcaagaat agctaatagg atcagccacc attttttctg
     6181 tcctttcttt ttaggatcag gcagaacttc ttcctcctct ggtaaggttt cgttttctgg
     6241 cttgtctttt ggcagaggca tagatttgaa tgaatcaggc attggtgcat cttccgtaag
     6301 gggtgtgata acttttgttt cattatccat cacatgaggc tgccattttt cttcgtttgc
     6361 acgagctggt gataatactg tagaaagatc atcagacatt tcctcggctg acttataacg
     6421 gtctgcaggt tctttagccg ttgcccgtaa aatcacgttt tccagtgctt gtggcacatt
     6481 tggatcaagt gcttttatag atggaagatc atcttgaaaa tgttttaaag caattgttac
     6541 agcagattca ccatcaaacg gtacgctacc cgtcaacatc tcataaagga tgatccctaa
     6601 agcataaata tctgactgct tcgttgccat acttcctctg gcctgctcag gggacagata
     6661 atgaacagat cctagcatcg tattcgtctg cgtgatcgat gtttcagata aagcgatagc
     6721 aatcccgaaa tccgtggttt ttacaacacc ttcattatcg atcagtacgt tttggggttt
     6781 caaatctcgg tggatgattt gatggttatg agctaaagaa atagcagaaa ggatctgctg
     6841 cataatattt actgctgttt cataaggaac aggataatgt gtttggatat atcgtttcaa
     6901 gtccattcct ttgacgtatt ccatcaccag atactgcatg ttgtcttctt caccaacatc
     6961 gtaaacacta acaatgtttg gatgaacgag ttctgttgct gctaaagctt ctctctgaaa
     7021 tcgtcgaatc gcagtctggt cattttgaaa gtcaaatcgt aagactttaa ctgctacatc
     7081 acgatccaag attaaatcgt gcgccaaaaa tacatttgcc ataccaccgc taccgatatt
     7141 tccagtgatt tgataacggc catttaattt tttgcccaac tcgatcatac actgtcctcc
     7201 ttatattcga taagaagaac ggtgatatta tccgctccgc ctgcttcatt ggcacgatca
     7261 atcagctcag ttactttatg agacaatggg ccttcttgat tgatgatcgt tcctatttct
     7321 tcttcagaca gcatgttcgt caagccatct gaacataaca gcagtcgatc ttttagttgc
     7381 caaagatgag tagaaacatc tacttcgact gttccaggca tgccgacaga acgtgttaaa
     7441 atatttttcc ttggatggtt cactgccatt tcttcgctga tttctcctga tttgactagt
     7501 tcattgacta aagaatgatc ctccgtcaat tgcatgattt tatcttcacg aatcaaataa
     7561 gctctgctat ccccaacatt ggcaatcgtg aaccagtcat ctgacagagc ggccgctacg
     7621 attgttgttc ccataccgtt atattccggc tgttcttgtc cacgctgata aatcagcata
     7681 ttttcttctt gaatcgattg aatgaaccat tgggcgattt tttcatcgtc aacagcttct
     7741 tgtttttccc aagcttcgcc tagattcgtt accgccatct gactggcaat gtctccggca
     7801 cgatgaccac ccatcccgtc tgctaataca gcaagcttga gacctgaacg gctggtaaat
     7861 acactggcat agtcttgatt tgtgttacgt cgtcttccta catctgattg atattcaatc
     7921 tgcatttttt cacctcatta ctttttgcgc aaacaacaaa tgaaaaatcc atctgtgtaa
     7981 aactgatgcg gatacaaagt caacattttc tcttcgattg atgattgtag cataggattg
     8041 gtcaaaacat caatcctctc aaaatcaggg tgtctttcca agaaagcaga aacaacgtct
     8101 tggttttctt cttttaagat cgtacaagtg ctataagtca gaataccgga agactttaaa
     8161 gttggtgcgc agctttctaa aatttccaat tggatttttg gtaaattcgc taattcatcc
     8221 ggctgttttt tataacgaat atctggtttt cgccgaagca gcccaagacc tgaacaaggc
     8281 gcgtctacta aaatccgatc aaactgctct gcttcgaact gtttactgac ttctctagca
     8341 tccatctttt gagcgtagac agcagaagag acacctagac gtgctgcatt atcttcaatg
     8401 agtttgactt tgtgttcgtg tacatctaaa gcgaccactt ctccaccgca tgacggatct
     8461 aaaaaagaag caatatgcgt cgtcttacct ccgggtgctg cacaggcatc taagacatga
     8521 tgttcaggct cgatttgcat agacggcgct acaagcatcg aactttcatc ttggatcgtc
     8581 atgacgccat cgtggaacaa tgagctagaa gctaaatgcc cttttttcgc tactataccg
     8641 tatggactga cttggctttt ttcagctgtg atctcttctt cttgcaaaac agtgatggcc
     8701 tgatctctgg aaagtcggcg tgtatctact cgggcactag catggctagg ctgatatagc
     8761 gataaaccga gcttttcagt ttcttcgtat ccgatttgat ccaccagtcg tttcgtcatc
     8821 catagaggca ggctgatttt gacagaaagt ctttcgatag gatcagcaat gccttgcaga
     8881 tctggtgctc cttgacgctg ataattcctc aagacaccat ttacaaattt ccctgtccca
     8941 ggatttcctt ttacttttgc gatttcgacc gcctcgttta aaatagcatg tgcagggact
     9001 ttatctaaaa attctaattg gtatacagaa agataaagaa gcatcttgac ccagtcatcc
     9061 actttttttg ctttagcgat caatggtgca acgtaaaatt ccaatagtcg ctgatggcta
     9121 atcgtaccat agatcaattc tgtaaataaa cccacatctt tttgatttag ctcgctttgt
     9181 ttgatcatct cattcaacaa aagattcgag taggcgccac ccttgttcac tcgctctaat
     9241 gtcagtaatg ccatatatcg tactgatttt ttcatttttt tagggatttt tttcattatt
     9301 tcacctgctg accaatctga acttgctgac cgcttccgtt aaggaaatca tgtacggcct
     9361 gttttccttt accagctggc tgtaattcat tgatggctaa gacggtctgt ttaccgcaag
     9421 caatccatag attttttttg tctttccgga tgatcgtacc aggtgctttg tctgtagttt
     9481 ctgccaatac ttcaaccgat aataatttcc agcgtatgcc ctcataggtc gtgaatgcaa
     9541 ttggccaagg acgcattccg cgtacttggt tgtcaatttc ttctgcagtt ttatgccaat
     9601 cgattgcttc ttgttcccgt gtgatgtttg gcgaaaaagt tgcttctgac tcgtcttgag
     9661 gtactggttt ttgtccatcc aaaatattgg gcaatgtttc tagcaatagc tttcttccta
     9721 aaaggctaag cttttcaaac atcgtcccta catcatcttg tttagtgatt ggtatgcttt
     9781 cttgcgcata gattccgcct gcatccattt ttttgatcat ttccatgatc gttacaccag
     9841 tttctttctc cccattcatg atggcataat gaacaggcgc acctccccga tatttaggaa
     9901 gtaaagaagc atggacattg attgccccta attttggtac ttgcaataac ttttcaggca
     9961 agaattggcc aaatgccgca gtgacgatca catcaggcac caattcaatg atttcttcca
    10021 tttctggcga accggagatt ttctctggct gcagcacacg gataccgtgc ttcagagccg
    10081 ctttttttac cggtgtcggc gtaattactc gtttccgccc aactggacgg tcgggctgtg
    10141 ttaccactgc ctggatctcg taacctgatt ctatcagccc ttcaagaatc ggcacagaaa
    10201 aagcaggtgt gcccataaat actagcttag tcatcaagat gttcctccat atattcttca
    10261 agttcttctt ccgggatctg ctgtatcatt ttctcaatga acaagatacc gtctaagtga
    10321 tcgatttcat gctggaaggc acgggctaaa tagccaaaag ccgtcacttc tatctcttct
    10381 ccgtctcgat cataataacg cacggtcact tcatctgctc gcttgaccgt tccatagaca
    10441 tgcggaatac tgagacaacc ttctacgtca aggctttcac cttttgcttc gatgatttct
    10501 gggttaatca gttcaaattt ctcgccttca tctacttcga tcacagcaat tctcttgttt
    10561 tgtcctactt gaggagcagc gatccctatt ccgtcattcg caatcattgt ctcatataaa
    10621 ttgtctaata aagcgattgt ctcgtccgtg attacatcga ttggctgagc agttcttttt
    10681 aatttatcat tagggtggat caatatagga tagcgcataa tttggccctt tcttaaataa
    10741 agtttaatgg ttcgttgtca attgccacaa aaagaccttg tcgctgttct ttctggcttc
    10801 cattcaaaat ttcatccaaa acatgaggaa gattcggttc atgtttatat ttgatgatta
    10861 gttgataata atatcggttt ttcactctca aaatcatgct gggcgttggc cctaatagca
    10921 agctttgagg gcttaacact tcttttagct gattggcaat ctgaaagatt ttctttgccg
    10981 ctacttgttc ttcaggatgg ctggcggtta ttttcacagt aaagtaataa ggaggatagc
    11041 ctccacgatg ccgtacatgc atttcctgct gataaaactg ctcataattt tgttttttag
    11101 ccaattctat ggcgtaatgt tgaggattga atgtttggat cacgacttcg cctgcttttt
    11161 ccgctcgccc tgctcgtcct gaaacttggg taagcaattg gaatgtatgc tcactagaac
    11221 gaaaatccgg taaattcagg gaggtatcag cattcaacac cccaactaat gtcacttctg
    11281 gaaagtccaa acctttagca atcatttgtg tccctaataa tatatctgct tcttttgcac
    11341 cgaatttttg taaaatctgt tcgtgtgccc cttttcttcg agtggtatct acatccatac
    11401 gcaaaatcct agcttgcgga taaagttctc ggagttcctc ttctactttt tgagtacctg
    11461 ttccatagta gcggatttta ttgccgccac aattaggaca gcgattggga ataggttctt
    11521 catgtccgca ataatggcag cgcattgaac gtgtatccat atgcagtgta agagagatat
    11581 cgcaattagg acatggaaga acaaatccac agtccctaca catcacaaaa gacgaatatc
    11641 ctcgacgatt gagcagtagg acagtttgtt cttttttgtc taatcgattt tgtatttttt
    11701 cctgcaaatt agcagaaaaa gtagaggtcc gatgattctg aaactcttca cgcatatcta
    11761 cgatctcaat agctggaagc tgtgcagctg ctttggctcg gtggtttaac tgcaataact
    11821 gataaacgcc tttttgagct cgtgctcttg attctaaaga gggcgtcgcg cttcctagaa
    11881 ctattgggca atgatgatat ttactgcgcc aaattgctag atctctcgca tgataacgcg
    11941 gtgtctcatc ttgtttgtaa cttgattcat gttcttcatc gataatgatc agcccgatat
    12001 ttttcagcgg ggcaaaaata gcagatcgtg cgcctactac tacttgcgct tcttcccgct
    12061 caatcttgcg ccattcatca tatttttcac cttgggaaag tccgctatgt aagaccgcaa
    12121 ctgattctcc gaagcgtcct ttgaatcgct cgaccatttg cggagtcaat gcgatttcag
    12181 gcactaacat aatagccgtt ttattttctg acaaaacatc agcgattgcc tgaagatata
    12241 cttctgtttt cccgctgcca gtgattccct ccagcaaaaa gacctgatct ttttgctgct
    12301 gacctgctgt gatgatttgt tcgacagcat ttttttgttc tgcattcagt tccaatgccg
    12361 tcgtttggtc aaatactcgg tctttataag ggtcgcgata acgttctgat tctataaact
    12421 ccagccagcc cttttctttt ccttgattca agacagcagt actgattcct ttatttttga
    12481 aataagcaat tggttcttct tggctcagtt cagataaaca caaaagaagt tctttttgtt
    12541 tcttggcatt ttgccgtaat tttgcccatt cttcttcgat ttgcttttca gttaacaaag
    12601 aacggaccaa acgaatcgtt tttactttat ttcgtgtatg gacttcatat ttgatatcta
    12661 ctaattgctc ttggcgccag cgaatcaatt gagaaaggct gccttcttct tctgcttcct
    12721 tccaggaaat cacttctcgc tgatcaaaca atacctgaac ttctggtgtg tctgccaaag
    12781 gatagatcaa tttatcatat tctgctttca tcacactcgg aagcatcgtt tgtagacaag
    12841 tgattttgaa tgcgtaagta atttttttca tataatcagc taatgaaagt aattcttcat
    12901 tgaccaccgg agcaaggtct agcaaacgaa taactgcctt caaagatgga ttagctgatt
    12961 cttctgattt ttggatagcc ataacaaatc cttggacatg acgattgcca ttaccaaaag
    13021 ggacttctac tcgcatcccg acttgcaaag ctgtttccat ttcagaggga accaaataag
    13081 tgaatggctg atcggtctgc atagtaggta cgtccacaat gacttcagct gttaatgtca
    13141 tggttcgttt cctccttttt ttgtcttttc ttatcaagcg acaaaaagtt ccttgcctat
    13201 tttactagag atcgatggat ttgtcttatc ttctgccaaa tcctaacaaa aaattaacag
    13261 cagataagaa aaaaaagaac tgtgtcaaaa agttcagaca cagccttttc taccagacaa
    13321 taagtggcga atgtttgaaa aggaaacagc tgggtttgct tttcttttta gatgttcgct
    13381 ctatttttct ttattacatt ttttgctctt cacggatacg tgattccaat tcgtgttgtt
    13441 cacgctcttt ttgtgcttga cgttcctctt cttccatctt caaacgttca cgttttaatt
    13501 caggatgtgg atcattgact acatttcctg catcgatttc ttctaaagct tgaccgacgc
    13561 tttttactga atcaaaagaa tctagagtag gctgtgcact tgcatccaat tcatgcgcgc
    13621 gtttacttgc taaaatcact agagaatatt ttgaatttac acgatctaat agtgagtcaa
    13681 ttgaaggttt taacatcata agactacatc tcctttaaca ttttaatata tttaccgatt
    13741 acgcgatcta ctcgaaaatg ttcactggca ataatgtttt tgattcgttc aactgctagc
    13801 ggcacttcat catttacaac agcatagtcg taaagagcca tcatttcgat ttcttctttt
    13861 gcaactttca ttcgtttttc gattacttca tctgcatctg ttcctcggcc tacgatccgt
    13921 gattttaatt ccgctaaatc tggcggagtc aaaaagatga atacaccatc gggcactttt
    13981 tctttgactt gttttgctcc ttggacttca atctccaaga aaacatcttt tccttcatcc
    14041 aatgtcttgt tgacatatga caaaggagtt ccataataat tcccgacata ttctgcgtat
    14101 tccagcatct ctccagcttc gatcattgat tcaaactctt ctttgcttcg gaagaaataa
    14161 tcaacgcctt cgacctcccc ttcacgcatt ttacgggtag tcattgagat cgaatactga
    14221 aaatcattat cctcactctc aaaaattgct tttcgtactg tgccttttcc tacaccggat
    14281 ggaccagaca acacgattaa taatccacgc tctgacatga cgacgtcctt tcaactagtc
    14341 tttttgtact tatcgtttat tttgcacttt ttttctgcaa ctttcaaggt tatctttaat
    14401 tatagctaga aattggtaaa aaagctaccc tgtaccaaga ggtcgtaaaa aagctgtcct
    14461 gcatcaagta ataagaacag ccaaacataa acagcttctc atgtttttcg tttggttggg
    14521 gttattatcg cagatgcaag cttttgaacg ccgtgtataa gaggtcgtga agaagccgat
    14581 tagtttcgag tatgaagaaa aattttgtga aaatagtttc tcacattttt gcataaattt
    14641 ggcttaatac cgagaaaatg gtttttgaac atcacttatt caaaataaaa aaagattgtg
    14701 acacgaattt tgtcacaacc tttaaatgta ttcctagagc aaagaagcta agcgaagaaa
    14761 tccaattctg tttatttatt gtatttcttt gttcagcatc cttgctaaaa gcccatattg
    14821 gacaaagtat tcactacacc catgatctca tcttttcgag aacgtgcttt ttctgtcgga
    14881 tcttctacct cttctgtgtc aattcgaaca ataaatttcc gattcatcgt cacgtaagca
    14941 ccgtcgttga tcaaaataaa cagttcgttc tcattatagt ctgcacaggc atctttccat
    15001 acgtcaaagc cgtttttttc agaagggata tcccgtttaa ccacagtttt tccacttaat
    15061 tcgtggaacg ttacgtcata atatttcatt atctctacct ctttcgtcat tcaatatttt
    15121 gtacttgttc tctgattttt tctaagatcg tcttagactg gacgacttga tttttaattt
    15181 cgattgcatt tgatttggaa ccgatcgtgt tgatttcccg attcatttct tggatcagga
    15241 aatctaattc ccgacctaca ggacattgtg cttcgataag ttcatctagt ttttgtacat
    15301 ggatcgccag acgatccagt tcctcgtgaa tatctccgcg ttccaacaag atggctagct
    15361 ccgtcaacaa ccgttgctca tcgactttcg ctccaagaaa ttcctccagc tttttttgat
    15421 accttatttg aaattccttt tcatagattt ctacaaaact atcgagtttt tttagtactt
    15481 ctttgaattc ttcagtattc tttttcaaca ctaaagctaa agcttgacct tctttttctc
    15541 gactcgcctc aatagaatca agtgcgcttt tcaatgtatc tagagctaac tgtgccaatt
    15601 cttccatgct ctcttccggt ttttcttcta ccgttacgaa gttttcgtcc agcacgaagc
    15661 gttccgccat ccgactaatt gacacttgat tttgactacc gtatcgttgt tgaattccgt
    15721 ctgtcaattg agtgataagt gaatcaatca attcccagtt gatgacgatc tgtttttcgt
    15781 ttttgccaat ataagctaga ttcacaaata cttcgattcg accgcggctt aactgctgtt
    15841 taataagctg acgaaaatcg ttttcaacaa agttcaggat tttaggactt cgtatttgaa
    15901 catctagaaa acgctggttg acacttttta tttcaatatc taactgatac gcttttgttt
    15961 cgcggatggc tttgccaaaa ccagtcatac ttttcattcg acagggtcct tcctcattac
    16021 atattttcta ttgctttttg cagttgttca aactcttcgt tcgtcaatgt caggcctttc
    16081 cccatttttt catggtctgg cgcccaatct cgaagatcga attttggcgg gcgaccattc
    16141 cagctgacta ggttcagttc tttacgccac cccttgttgt tttctgacaa tatagcgatt
    16201 tcttctacga tttcatatga aaattcttgt gccatggttg ttcctccttg agataatata
    16261 aatataggat cagtaatgtg tcaactggtt gcttaagcaa ttcatgctgc aaacgatcct
    16321 tgcgttttcc ttgtagcaga taatcaaaat aaacagtttt cgtgcgctca ttttgtattt
    16381 tttgctggta ttttcgatac cttcgccatt cttcagtact taattgacca atttgtgcca
    16441 ataaatcatc cggcatactt tttatatctt gttggcagat catttgaatc atcatgtcta
    16501 cttgatagat ttcattgggc agtaaaaaat gtttttttgc taaaagttct agcatcgcca
    16561 atccgcatgt ctctggtacg accctgttct tatataaatt acgcagtttt tgccaaaatt
    16621 ttatgctttt gaaaaaagat aattctaact gaatttcttt tgttaagtaa gacaaaaatc
    16681 ctcgccagta tgttcttgca ggtttaacag aatgaaatgt ataccagttc ccatagtaat
    16741 agcctaatcg ttttttcatt tccagatagc ctaattgtaa atttaattgg ttcctttggc
    16801 tatcaaaaag cagaaagctg cctagtgtcc acaatgtttg acatttccaa tggataaaaa
    16861 tgtctggcat acgaattttt ttcgctgggc ctggcccttg cacatctacg acaaatgcct
    16921 ctgttgctcc tttgttaatc gcaatatcaa taggtatttt gttgcgatac ccaccgtctg
    16981 catatttatt ttttccgatc tttcgataag ccatagcggg ataaaatgaa gcagaggcga
    17041 gtatccaatc tggtatttct tctgtttttg tctgttggat atcagtcact acttccgcta
    17101 gttctggcag tttagtagac accgtatata aacgtatttt gggattatgt ttgagtaagg
    17161 acaaatccaa attgtctttg accatctttt ccaatggagc agaagaaacc ccgccttcaa
    17221 ttagtgctgt tttcgtcatt tggcgtcttt ctcgatagaa gcgttcacga aggtcttcaa
    17281 ctgcagccat ttctggtaac gcaagcactt gtcttgtcga cagctttttc cataaattca
    17341 cagctttttt catatctcct tgtaaaatca atgcgccgtt caacgcacct accgaggtac
    17401 ctgtgataat ctcaaagttg atatcatgtt ccttcagcgc ctgccacaca ccaatctgat
    17461 aagcaccgtg cgctcctcct ccacctaaaa caagtgctgt atgatagacc agctttttat
    17521 ttaattgtgt ggttccttga tagccgtttt tatgaaaaat acgcaaccat tcttctgata
    17581 gaggagattg aaataaaaaa caacagtttt ctttaataaa aaaacgagcg caagcttcca
    17641 tataccgaaa aaattttttc cattcaaaag tttcaccttc aatcaacaga ttattaaaaa
    17701 taacatctcc tctactttta gcaatagtaa aatagacggt atattttttc gatactgctt
    17761 taaaagcaca ttgctttttt gcagaacgcc aaaaagcgtc tctactttct tgatgacttt
    17821 gaaaaaatgg aaggggttgt tttgcttgct ggataggcga atacagcgac attttctgtg
    17881 aaaagtaaac tttttttatc tccatcgcgc tctccttttt cttcaagtat accagtacgt
    17941 cataaaaagg tcatgagtct cgtattcgac tttgtagatt ccataagaaa acaattcata
    18001 aaacctcctt taaataaaaa aatacaaaaa tcacaaaaat taattgaaaa tgagagtgtt
    18061 ttcaatgaaa acgtgatttt gtaatggaat tttaaaataa aagaaacaag cttttttaat
    18121 gcaaatgttg taaaataaaa gagattaaga ggaaataaga ggaggaacat gatgactgag
    18181 tcagtttatg tacatattga taccacaagt aatgctgttt taacaaaagg gctgactgct
    18241 actgactttg caaacagtat cgtgcatttt cctcaaaact tgctcttgct tgatccatct
    18301 gcttctgctg gagaatatga atcccataca gggctgaaag taatccgtgg tacagaaaat
    18361 attcagcgtt tcttttcctc ttctcgtaac aggcgtggat tcgatgaatt gaagtggatc
    18421 gactttactg atttaacgat gctgaaagaa ttgacacctt tagaaatttc agaattatta
    18481 tattttggtc atatgaaaac acatcttcat tcgccgtttt tttataaact acagaataat
    18541 tttgtatact tcgatcttaa tgatcagctg aatcggattt attatcgcta tttagatgaa
    18601 ttttatagga tttttgctag taaattaagt tcgatcttgt cagataagat caatgtaaaa
    18661 aaatcttttt tcaaaaaaga aacacctgtt gaaaaaatca gtgtggaatt attgaaagaa
    18721 atgcgtaatt tgatgcagga agggatcatt ttttcttttg atcaagttgg tttgatcgat
    18781 ggtaaatatc gtatcccaat ctatgtgatt gaagattata tttggaaatt aaaaggcacc
    18841 cggtatcgag aagaggaaat catcgctact ttaatctact ctgtcaccga tcgacactgg
    18901 caattggtag aagatgccga tctttcttat ctgccattga agtagatcta tttcttatca
    18961 aaaaataaaa aggaaacaag aaactaggac atgattctgt ctcaagtttc ttgcttcttt
    19021 tttatttaca gctcctgtcg tttatctggc acaaaaatcg aaggagtcgt ttgttggatc
    19081 cctaataaaa cgatgatcgt tacaatagct gtactcaacc cgctggcacc attcattacg
    19141 aaagaaaaga gccaaggatt catcccccat agtgcatacg ctccccagaa aaccactcct
    19201 gcaataaaat gccaaaaata tctggaaagt gcgccaatca atgcaccaaa cacgatgctt
    19261 tttctaattt gtttcgtatt atcgtctaca tagccttttt ggacatttga agccatgagc
    19321 cctgctgcgc ctgcaaaagt ataggcaatc gggtactcaa tcaacacttg aacgacactt
    19381 aaataataaa cttgtcccaa cggaaaatgt aggatgcccc aaatgaaacc tgccagcatc
    19441 cctgcctttg tccctcttct taacgcaaaa agagtcaatg gaatctgccc taatgaaata
    19501 gaaaaactgc tgccgatctg gatcggaatc aacgacaaca ccatcgctaa cgctgcaaca
    19561 atcgttcctt ctacccaaac tctatttttt cccataaaaa aacttcctcc tttgaataaa
    19621 aaagtcacaa aggaggaagc agctttcgaa aatagtcaaa cagctgttta ttcacaattc
    19681 ctacgctcgc ataaccgata caggtacgaa gggtttagaa atagattcaa tctcagccca
    19741 attggactcc cctttgtgag tttatttatt atttattttt tgtttcaaac aatggactaa
    19801 ctggtttgtt ttcatggata cgtttgattg catctcccat tagtccgcca acacttactt
    19861 catcgatttt gtcgatttta cgatcttctg gaagatagat cgagtctgta acaactagac
    19921 gctcgatagc tgaatcttct attctttgta aagcagggcc ggataatact ggatgtgtac
    19981 atgaagcata cacactagtt gcaccagctt ctttcaaagc attggcagcc aatgtgatcg
    20041 tacctgctgt atcgatcata tcatcgatca gtacacaagt tttgcctttg acttgtccaa
    20101 tgatattcat tacttctgca acatttgctt ttggacggcg tttgtctatg atcgcaatcg
    20161 gagatttcaa aaattccgct aatttacgtg cccgtgtgac accaccatgg tctggtgaaa
    20221 caacgacaac atcatcgcct ttgattcctt tttctaagaa ataattggca ataagtggcg
    20281 cacccatcaa atgatcgact ggaatatcaa agaaaccttg gatttggact gcatgaaggt
    20341 ccaaagtcag cattcttgtt gcaccagctt tttgaatcat gtttgctact aatttcgctg
    20401 tgattggttc ccgtgcacgt gctttacggt cttgacgcgc atagccataa taaggcatga
    20461 ctacgttgat tgtttttgca cttgctcgtt tcagtgcgtc aatcatgatc agcaactcca
    20521 tcaagttatc attgactggg ctgctcgttg attgaataac atagacatgc gatccacgaa
    20581 tactttcttc aatgtttact tgaatttctc catcactaaa ttgagtaaca gatgattttc
    20641 ctaactcgac gccgactgct gctgcaattt tctctgctaa tgggcgattg gaatttaacg
    20701 caaaaatttt taatcttggg tcaaagtaat gttttgacat ggggacctcc actttctttt
    20761 tcacaactct agaattctac ctaataaata gtagtcatat tttttaaata aatcaagtga
    20821 ttttgcaaaa gtcttagttt agataaggga gtttttttgc atatccttct ttattgactt
    20881 gtcttgctct cgcaattgcc agcgcatatt ctggaatatt gtctgtaatt gttgacccgg
    20941 cagcaatcga actgttttta gctacctcga caggtccgat gatattcgtg ctagaaccaa
    21001 taaagctgtg gtcgccgaca gtcgtatgat gtttgttctt tccatcatag ttgacgaaga
    21061 caacaccaca tccgacattg atttcttctc ccagcgtagc atcaccaaca taagtcaagt
    21121 gaccgacttt agttcctttg ccgatttgag catttttcac ttccacaaag tttccgatat
    21181 ggacgttttc gccaatttca gcttttggtc gtaaatgtgc ataaggtccc acatctgcat
    21241 gtttcttgac atggctgctt tcaatgactg agttttcaat gacgacgtga tcctcaatcc
    21301 tactgtctac gatttttgaa tgtgacccga tgacacaatc agaaccaatg actgtatttc
    21361 cttggatttg tacacctgct tcgatcaatg tgtctggacc gatttctacg cctgcatcga
    21421 tgtatgtcgt tgctgaatcg acaaagctta cgccattgac catatgcatc tggttaattc
    21481 gctttctcat gatctcattt gctttagcta acgcgatccg atcattgact cccattgatt
    21541 cttcaaaatc atccgtttga taagcagcaa ctgtatggcc ttcttctttt aagatttcga
    21601 tgatatctgt tagatagtat tcaccttgag cattgtttgt tccaactttg cttaacgcat
    21661 cgaacaaagc ttcgttgtca aaacagtaag ttcctgtatt gatttcttgt acaagagctt
    21721 cttctggtgt tgcatctttt tgttccacga ttttttctac gatcccaata tggtcacgga
    21781 tgattcgtcc atatcctgtt gggttttctg cttgtgccgt caagatcgtt gcacttgcat
    21841 tttttccttg gtgatattca aaaagattgt tcagcgtttc agtagtcaat aatggtgtat
    21901 cgccactgat aactaacgtt gttccttttt ttccttccaa gaaagaagca gcttgcaaaa
    21961 cagcatgtcc tgttcccaat tgttctgctt gtaacgcata tttactgcgt tctcctagtt
    22021 gtgctttaac ttgttctgct ccgtgtccaa cgatcgtaat cacttcatct ggtttcgttt
    22081 cactgacccg attaatgata tgttctacca taggtttacc agaaacggga tgtaaaactt
    22141 tatataattt tgacttcatg cgggtacctt tccctgccgc caaaataatt gcataacgtg
    22201 cgtccaactg cgtcactcct aaatatagtt tgctgatttc agtttatccc agtataccca
    22261 ttttttcaac aaaacaagtg attttctttt atcccagcgt tttctggata aaaaaataga
    22321 tagaccataa cgggcctatc tatttggtaa ccggtgctct cctgccaact cctcggtcac
    22381 aagtcacact actcataaaa catgaaaagc tgttttttga aatcgtttct tgtgacttag
    22441 agttaaacgg taggcttaca acctcttcat taaacgtttt tcaatcagct gaccttcggt
    22501 attagctcaa taaacggcaa aatatgagga gcaatttttc cgtttattga gctaatactc
    22561 aggacaggac gctgatttca caacctctag ataaacggtg ttcaaaagcc agtttctcgg
    22621 tattaagcca aatttatgaa aaaatgtgga aagccatttt cacaaaattt ttcttactac
    22681 tcgaaactaa gcggcttttt cacaacctct tattctgaaa aataatttcc tggcacaacc
    22741 gtaatatttt tcgtttgagt atctacatct ttgacataaa gcagtgacgt ataatcatcg
    22801 atggcgcgat gaccgttgaa ttttgattcg gcaaatacag tgatgccaac aagttctgat
    22861 tcgaattctt caatcaagct cttcattccg ttgactgttc cgccgccttt catgaaatca
    22921 tccactacaa ggacacgtga accacgtttc aaacttctct tagacagttc catcttttca
    22981 attctttcag aagatccaga cacatagttg acacttactg ttgatccttc tgttattttt
    23041 gagtcccggc gtacaataac gaatggcgca ttcagataat aggacacagc ttgggcaata
    23101 ggtacgcctt ttgtggctac agtcattaca gcatcgattt tttcaccaag gtatttagaa
    23161 gcaatgattc ttccaacttg acgcagcagt tcaggttctc ctaacaaatc agagagataa
    23221 acatatcctc ctggaagcag acggtcttgt tctgatagac gttctgctag agattcaata
    23281 tattttttcg cctcttcata tgaaatttca gggatgaaca atacaccacc tgcagcaccc
    23341 gggattgttt ccaaggttcc ataccctcgt tctttaaatg ttttcttgat gattcccaaa
    23401 tcttcactga tagaagattt agcagattca taacgttcgg caaagcttgt taaggaaatc
    23461 aattcatgcg ggtgattcaa taaatactgt gtcatgtcaa ctagacgttc actgcgacgt
    23521 actttcacct attccaactc cttttaagaa attttatcgt tttacattat ataagttttt
    23581 cttcaaaaat tcgagcattt tatgaaaaaa taagaaaata gttcggtatt tattaaaata
    23641 aaaagaagcc tataaaaaat ttttcgtttt tataaggctt cttttcttta taatttagtt
    23701 attttttctt ctgaaggcaa ccaatattcg tttgatgata tttacaatca ggaacaaagc
    23761 aataaagact aatgtgattg ttgctcctgg aggtgtatct agataaaacg agctagttag
    23821 tcctgacagc atccctagaa atcctacaca aacactgata gcaatcacga cattgaagct
    23881 tttgcctaat cgcataccga ttgctgcggg aagaaccatg attgcggata tcaaaagtgc
    23941 accggcaatc gggatcatca cagcaatagc cacaccagta atcacattga acatcatcga
    24001 catcaaatga acgggtagcc catccacgtg ggcggtatct tcatcaaaag tcagaacata
    24061 catcggccgt ttgaataaaa agaacaatat accgatggca acgaataaga ttcctaaaaa
    24121 gatgacttgc tcccatgtaa tggtcacgat cgaaccaaac agataggatt ggatacttgt
    24181 tgctgaattc cctccgctca aattcatcaa tactaacgct agcgccaaac caccagccat
    24241 taaaatggca atggagattt ctgaataggt tcggtagatc gttcgcagat actccaatat
    24301 cacagctgct aatacaacga ctagcatggt cgtaagtgta ggattaaggt tcaaaaagaa
    24361 tcctaacgct acccctgcta aagagacatg agaaagcgta tcagccatca gcgattgtct
    24421 tctgatcact aaaaacacac ctagcatcgg ggcgattccg gcaatgaaga tagcggcaag
    24481 aaacgctctt ctcatgaatt catatgaaag caacgccatt gagaatcctc ctttctcacc
    24541 agtcggatct gtctgtccgc atactgtttg atatcttcat gatcatgggt gatcattaga
    24601 actgatttcc catggacatg cgcactatgt cggagaagac gataaaattc atttctggat
    24661 tcttcatcca tacctgttgt tggttcatca agaacaaata agtcagggtc agtagcaaaa
    24721 atacgcgcta aactgattcg ctgcttttgt ccgccagaca attcacctat tcgccgattt
    24781 cgcatatccc acatccctac agcttctaaa gcacgctgta catgttgatg atctttttca
    24841 gagagacgct tgaaccagcg gcctcttgga tatcttcctg aacggactaa ttcgatcacc
    24901 gtactaggaa aacctgcatt gaaagatgca acttgttgag gaatataacc gatacttaat
    24961 ttttttcctt cttgattttt ctcagcaatc tttatcattc cttttgaagg tttcagtaat
    25021 cctaacgatg ctttcaccaa ggttgacttg gctgctccat tttctccagt taaaatgaca
    25081 aattcacccg catctacatg ataagagaca tcttctagta caggttcatc atcatagtaa
    25141 aaggtcaaat cagatatttc gatataacgc accttacaac ctccattcta aaacttaatt
    25201 gatactttct tttaaagctt tcagattttt ttccatgaca gaaatataat tttcgcctgc
    25261 tttgatttgt tcattagtca agctttctaa aggattcaac acttctagct tcactcctgt
    25321 ttcattcgac aatgtctcag ctaccttcga agaggcattt tcttcaaaat aaatcacttt
    25381 gatgttgttt ttcttcacaa attcttttag ctcagctaag cgacttggtg tgggttcctg
    25441 atctggtgaa acgcccgaaa tcgctacttg ttttaagtcg tattgattcg ctaaataagc
    25501 aaaagcagca tgctgagtga caaatgtgcg gtttgttgca tcttttagct catcggagta
    25561 tttttgatcc agtgcttcaa gctttttgat ataagcatcg gcattcttcg taaaagtttc
    25621 ctttttatct ggatattttt cgcctaattc ttcagcaatc gttctgactt cttttatagc
    25681 taataccgga tcgagccaaa catgtggatc gtattcatgt gaatgaccat gcccttcttc
    25741 tgattcttca tgttcgtctg cttcttgcga ttctaaacgg tcgattcctt tagctgcttc
    25801 gatcatcaag gtctgtttgc tatccactga atcctttaac gaatccacaa aaaattccat
    25861 gtctgaacta ttatacacga aaacatctgc atcggaaatt tctgctcttt cttttgcact
    25921 tggttcgaaa tcatgcggct ccgttccagc agggatcaaa agtttcacgt ttccttcatc
    25981 ccctacgatc tcttttgtaa aatcatacat cggataaaaa gtcgtaacga tcgtcaaatc
    26041 ctcttttttg tctgcttcct tgttcgtatt tccacatgct gcaaacagta aagcacctat
    26101 cattaccgta gctgtcaaaa gatatttttt catttatatg ctcctttcaa tgaacaaatc
    26161 ggaattttac gatttattaa cgtgaactaa tgtatcaaaa taaaaaaaca cagtcaatag
    26221 aaatccatca atttctgatt taagcatgta tattcttgat taaatcgtaa aatggatgag
    26281 ttgctgctca tatctagcca tttcactaca ataaaaaact gaagaatacc attttaggat
    26341 tcctcagttt ttcatcacct attttacttc ttatatttta ttgcagggtt cgtactaaat
    26401 aaacttcctc acaaaaacct tttaacccat tatatactct ctgcgctctt gaataatgtt
    26461 gacacaaggc aaaaacggtt ggtccgcttc cactcatcaa tgcagcatct gctccgtact
    26521 tgatcatacg ctctttgatt tgatggatga ctggatgccg ctttcccgtt atatcttcta
    26581 aactgtttcc catttttttg atcatctgct gataatcttg ctctcggatg gcttcagcaa
    26641 gtccgtggat gtcttgatgt tggatggctt gcgtatctac ttctctaaac actgtacgtg
    26701 tagagacact cattcttggt ttgaccaaca caacccagca ctgtggcata gaaggtaata
    26761 cttggatttc ttcccctttg ccaccgacaa atgccgtact tccgtagaca cagtaaggaa
    26821 catctgtacc gacttccaaa ccgatttctg ccagttcttc taacgaacag cccagattcc
    26881 ataattggtt gatccctctt agagcagctg cacagtctgt acttcctccg cctaatccag
    26941 ctgctactgg aatattcttc ttaacaaaga tcttgacacc ggttttgata ttgtatttat
    27001 tttttagcaa taaagcagct tgataaacat tgttccgttc atctaccggt aaaaatgctt
    27061 tatttgtttc tacaatgatt ttgtcttctg ttatttcttc aaaaaataga tgatctgcca
    27121 agtctacgct cgacatcacc atttccaact catgataccc atcttgccgt ctatataata
    27181 catccagtcc taaatttatc ttggctggcg ctttttcaat caattccatc ctctcaccca
    27241 ttcgaaaagt ctaagtcgca agtattctat catagggaaa aataaaagta tagcttatgt
    27301 ctgcctagtg gttgattttg tcatcgtttc aacagctttc acagaatttt ctcttcataa
    27361 tccaaacaag ttctagtgat tcttataatt ctgtgagcac ttcttctgcc aaactcggaa
    27421 catttccttt ttcttttcct atcagactga gtagctgcat ggtagcaaat ccgaaattac
    27481 ttcctttcct ataagcaaaa taacgtcctt cccaatgatc gacatatttt aatttctttt
    27541 cataagcaga ctggaaggaa tagaaaaaat taccataatt atatacgata tgcatgactt
    27601 tttcttgaaa gaaagaaaaa tctgtttctc caacattagc taatggagct aagcctagat
    27661 tggcttccga gtagcccatt ttctcgtata caataaacag attggctaac agatagtcag
    27721 ctaattcctc agattcttta ggtaagaccc gcaacaaatc atatcccgcc tgcttcttcg
    27781 tcatcggctg agcggtgatg aagccaacga ctttttgatc tttacggaca ataccgacac
    27841 cgctagataa tacatatgat ttatcaaatc gtcccacaga gaagtatttt tctcgtgcac
    27901 cttgaagcca tttttgtgaa acttcattta cagcttgatg taattcttcc ggcagttgat
    27961 cgtaccaagt aaaatggtat ccttctttca tcaaacgttg atattcaagt tgctgactca
    28021 gccgagacaa gtcttctgtt gctagatcaa caatccctgc ttcacctact ttcgtgaagt
    28081 gaaaacccaa ttcatgaagc atcactacgt agctttctga tatcttataa aacgcaagct
    28141 gatagcctaa acaatctgct tgtttcataa actgattcgt tgctgcccgc atcattttag
    28201 gattgccaac tggatctcct aaaacaaagc atttgtttcc tttgatctga taaccaaaaa
    28261 cgacttgatc ttggtcgtct tcttggtaat aaaaataact gtaacctgac aagcgaagct
    28321 gatggcttgt agctgtgcct ccgtatgttt ccaataaacg gtcaaaacgg tctttttcgt
    28381 actcactgcc aagcttcgta gtatcttcag ctaaatattg ataaagcgcc actaaagcta
    28441 gcaatgagat gcccaaacca ataagccctg agaaccacac atcatctgaa ggaaacaaaa
    28501 taaaatgatt tcctgttatt cgaccgacta aaaagcgtcc gctgtgatag cctgcaattg
    28561 cgtaaacgat aaacaaaaag ccgaacaagc tagcatcaaa gattatagct ccccaagaat
    28621 aaacgaattt tgttcgataa aattcttttc tggataacca cacagctaac agaatcaaga
    28681 catataccaa tatcaactgc caagataacg tacgtgtgat tgtatttaaa ataccaaaag
    28741 atagtaaagc aatcgtaggc agaaatgcct ttttgacttt tactgaaagt gctctagcaa
    28801 ggccgagtaa taaaaagcca accaatagat tcactgtctg atctaaaaaa ttgaatgaga
    28861 acggcaataa tacttgaaat agccgactga ttgcagacag attagtgatt gttgatagaa
    28921 gtaccatcat gatccccgca aaatagacag cacttacgag cacgatatgc gcaacctttt
    28981 ggaaaaacag tcgcggaatg ttatctaaaa atcgattgat tttgactcct gtatgtgtga
    29041 tgaacaatag aagtcccgtc ataaaaggta gaacataata aaacaaccga taatataata
    29101 gccaaactac tgctacatct tgccgaacag acaattgact cattcctaag atcatcaaaa
    29161 cgtcaaaagt ccccattcca cctgggacca tcgtcagcat tcctatcaat gtagcaatca
    29221 tgaacatagg atagatcgaa agcatcgaaa tatctattct catcaacttg ccgatgatca
    29281 aaaataccgc aagggctccc gtccattgtc caaaggatgc cagtagcaga ccaagcaagc
    29341 cttttggata aaactcgaag aacaatgtct ttctctttag ataagcaaaa aagaaaatga
    29401 ctggtgctaa aaaactaccg ccgaaaagcc agatccaata gcttcgaaaa atactttctg
    29461 actgaataaa aaagacatca ataaatgtaa gaaacgacca tacagacaaa ccagtcagca
    29521 taaatagtgc gacttttgat acagtggcta aaaccatttt tctatttgtc tgctttccat
    29581 aaaaactagc ccgcagactt gctcctacca caccaccaaa accggcgaga ttgttgatcg
    29641 tgtttgttac ccacgcagca agaagaaact cttttctttc catttttggt tttccttgtt
    29701 tttctaagac tctgatcgtc acccaatcat acaacaacat cggagtgaca cccagcagtc
    29761 cagccagaat catgagcagg atcgtcatgc ggctttgttg tcccatcctt tgccaaatat
    29821 cttgccaact catgccatta gcaatatttg cgacttgatt tgccacaaag actaacacgg
    29881 agccgaaaaa aatcagtttt aaaatcagac tatgttcctt tatccattgc agcatcaagt
    29941 atcgtttatt tttcacattt tcccattcct aacttgtata cttttgtgtc ataccccaat
    30001 gttatcttat ttattttaat ttttccactt tagacctgtt ctttgcttgc gatgatagaa
    30061 aaagaaagat atcctaatgc taaaggagag gataacatga aagtaggatt tatcggcaca
    30121 gggaatatgg cacaagctat tattgatggt ttattaaaaa aaggaacagt ctcgaaagaa
    30181 gaggtgttcg tttccagtgg acattttgaa aatgcacaaa aatttgctgc tgaaactggc
    30241 gtccatgcat gcaaaagtaa tcaggaagtt gcatcacaat cagatatcgt tcttttagcc
    30301 gtgaaaccaa aaatcattga aaaagtattg gaagaactaa gagaatatag ccagaaagtc
    30361 ttattcattt ctattgcttc aggaaaatca ttaatagatt tagaaacagc gcttgaagat
    30421 cctcaggcct ccatcgtacg tgtgatgccg aatgtcaatg tcagcgtagg agcaggcgtt
    30481 tctgcgattt gcaaaaatga agctgccacg gaggaacagc ttcagcaagc tacgatgatt
    30541 tttgaagcaa tcggcagcgt ctatccttta gcagaatcag atttcagtac attcattggc
    30601 cttgctggca gctcacccgc attcgtttac atgctgatcg atgcgatggg acggactggt
    30661 gtactgaatg gtttgtccaa acagacagcc actgagattg ctgcaaaagc aattatgggt
    30721 tcttgtcaaa aattcctttc aagtgataaa aatccatggg aacttgtaga ccaagtttct
    30781 tctcctggcg gtacaactgt tgcaggtgta gttgccttag aagaagcagg attcatccca
    30841 gcaatcatca aaggaatcaa tacgacgatc gagaaagatc ttgagatgtt gaataaaaaa
    30901 taatgatagc aatgtttttt cgtgccttac aaaaaaagaa gagctgtggc ttttgccgcg
    30961 gctcttcttt tgtatttttt attatttagc ataatcagtt gcacgagttt cccgaataac
    31021 gattactttg atgtgtccag gataatccag ttcttcttcg attcgtttac gaatatcacg
    31081 tactaaacga acagcgtcta aatccgagat ttcttctggc ttgaccatta cccgaacttc
    31141 gcgtccagct tgaactgcgt aacttgattc tacaccggcg aagctgttcg aaatattttc
    31201 cagacttctt aaacgacgga tgtagttttc taatgattca cttcgcgcac caggtcttgc
    31261 tgctgagagc gcatctgctg cagctacaag gacagagata acagaagtag cttccacatc
    31321 tccatgatga gaagcgatcg cgttgattac tactgggttt tctttgtact tggcagctaa
    31381 ttccgcaccg atctcaacgt gagatccttc aatctcatgg tcaagtgctt tcccaatatc
    31441 atggagcaac ccggcacgtt ttgctaattg gatatcttcg ccaagctctg ctgctaagat
    31501 accagctaat tttgcaacct cgaccgaatg cttcaagacg ttttgtccat aactagttct
    31561 gaatcgcaga cgtccgatga tcttgattag atctggatga agtgtatgag caccgacttc
    31621 aaacgctgcc tcttccccgt attcgcgaat acgttcatcc atttctttac gagatttctc
    31681 gaccatttct tcgatacgcg ctggatggat tcgtccatct tgtatcagtt tttccaaggt
    31741 catccgtgcg atttctcttc ggattggatc gaatccggaa agaacaactg cttccggtgt
    31801 atcatcgatg attaaatcga taccagtcag cgtctcaagc gttcgaatat tacgaccttc
    31861 tcgtccaatg atacgacctt tcatttcatc attcggaagg gtaacgactg aaacagtcgt
    31921 ttctgaaact tgatctgctg cgcagcgttg aatcgctaat gataataagt ttttcgcttt
    31981 gcgatctgct tcttctttgg cacgctgttc agattctttt accattagag ttaactcatg
    32041 attcagctct tcttcagtcg atttcatgat gacatctcgt gcgtcctctt ttgataaacc
    32101 agcaattttt tctagttctt tttgttgatc ttcaatcatt ttttcaacgt ctttttctcg
    32161 ctcttcaatt aattgctgtt ttgaactaag tgcttcttct ttactttcaa gtgagttctc
    32221 gcgtttttct aacgagtcat ctttacgatt cagcgtttgt tcgcgttgaa tcaaacgatt
    32281 ctcttgagat tttaattcta acttactttc tctcagctca ctttcaactt cagaacggta
    32341 tttctgattt tcttccttag cttctaacaa tgcttctttt tttagagttt ctgcttcttt
    32401 ttttgcacta tcgatgattc cagcagctga tgattgtgca ccagctattt ctttctcgtg
    32461 acgtgacttt gcgaccataa accctaaacc aagaccgaca attaaaccga tgatagcgag
    32521 gagaatgttg aataccataa aatccacctc catactatct ttttcatttt tatcatctat
    32581 acagcttcta tgataaatta ttctaaactc gtcaatatgc atcttcgcat atatgtgtac
    32641 aaaaaacatg cacaacttta ttctaatgtt tgtaccccgt agtgtcaact ttaaataaac
    32701 aaagaaacgg ctttctactt attcattaaa cgatggtttt taaaataaat gacactagtt
    32761 tcatataggt aaacctttaa attgagaaac ttttctatta aattattctt tgtagatgaa
    32821 cagatatttt tattttatgt acccactcgt ataattaaat aactcacgat ttctttattc
    32881 aataaaaaaa cggcaggtca aggagagtac ctactcttca gaccagccgt ttttgtttat
    32941 cataattttt attcttctac taaaggcaat tcttcttgtc ctttttcatc ttctgggaca
    33001 gcgacttctt caccaatacc ataagctgct ctaactagag cagaaacttc tgccatcatt
    33061 tcaggatgat tagccatata gattttagcg ttttcccgtc cttgaccaat ccgatcttct
    33121 ttataagaat accaagcacc gcttttatca acaatgtctt tttctactgc catgtctaga
    33181 agttctcctt cttgtgagat ccctaaacca tacatgacat ctacttcagc gattttgaaa
    33241 ggaggtgcta ctttgttttt tacgactttg atctttgtac ggttaccaac aatatctgta
    33301 ccttgtttca attgttctgc acgacgtact tccaaacgga tcgttgcgta gaatttcaat
    33361 gcgcgtcctc caggagtgat ttctggattc gcaaacatca cgcctacttt ttcacggatt
    33421 tggttgatga aaatagcgat tgtttttgtt ttgttgatcg aaccagagag tttacgcaat
    33481 gcttgagaca tcaaacgtgc ttgtaacccg acatgtgagt cacccatttc gccgtcgatt
    33541 tctgctcgtg gaactaaagc agcaactgaa tcgactacta cgatatctac agccccactt
    33601 gatactaaag catcagcgat ctctagacct tgttctcctg tgtcaggctg tgaaagaagt
    33661 agttcatcga tattcacacc taatttttgt gcatattgcg gatctaacgc atgctcagca
    33721 tcaatgaaag cggccgttcc gccatttttt tgtacttctg caatagcgtg tagtgcaact
    33781 gttgttttac ctgaactttc aggtccatat acttcgatga tacgtccacg ggggtaaccg
    33841 ccgacaccta aagcgacatc taacgcaaga gagccacttg gaattgtaga gatttgttgg
    33901 tcgatttttt cgcccaattt cataatagag cctttaccat agctcttttc aatttttttc
    33961 agtgcagcat ctaaggctgc tttacgatca tctgccaata cgataatcct ccttatattt
    34021 acaaatcaga gcaacatttc attatcctta tcatatcttt tttagtgaaa aaaagcaaga
    34081 aaaaaccgaa caaatattcg catttatcat tttcttaaaa gctgttttct caaaagatca
    34141 aatccttgca taaccgcatt atatctgata gcagcacgat ctctggcaaa gtgatatttt
    34201 tccgaaacca cgccgtcttt agatgaaata gcgatccata ctgttcctgc agattgacct
    34261 tctaacgtgt ccggtccagc cactccggtg aaagctacac tgtaatctgt tcttgctatc
    34321 tcacgtgctt gaatggccat ctgctccaca cattctttgc tgactgttcc atattctgct
    34381 aatagctccg gatcaatctc cagaaaacca gcttttgttt ggagcgagta ggtaacgaat
    34441 ccgcctggaa atacttcaga tacaccagca atgtcgccta atgcggcttg gaaggcgccg
    34501 gctgtcaaac tttccgctgc tgttacagtc tgcttgtttt ctttcagtag ttccactacg
    34561 acttttgcta gcgaattatc atcgccgtat ccataaaaat attcccctac tcgttcttgg
    34621 attttttctt ctaatgctaa aagagcttga ttccctgcct gaacatcatt tgttttcact
    34681 gtcagtcgta atgtcacttc atttggtttg gcatatggtg cgatcgttgg attgatctgt
    34741 gtctcaatca gatcttttag ctcagtgacc agtcgcgatt caccaatccc ataaaagcgc
    34801 aaaactttag aaattagttt ttcttcagaa ggaaaacgtt cctctagtaa aggacgtact
    34861 tgttccacaa acattggttt caactcatta ggcggaccgg gaagaaggat atacgcatgt
    34921 gtatctgttt gataaaaagt ccctaaagca agccctgtac gatttggtag aggaaccccg
    34981 ccttcgataa tctgggactg ttgcagattg tttttggtca tttttcgatg ggttgtttca
    35041 aaatatgcca ataatttttt gtatccttcc gtattttgta tgagagactt accgaggtga
    35101 gccgcagttg cttcttttgt caggtcatct tctgtcggtc ctaatccgcc acataagagt
    35161 atgagttcac ttcttgtttc agctagagca atcaactctt ctaaacgctg ctgattatct
    35221 cctacaactg tttgataata aacttcgata cctagatcag ctagctgttc tgacaagaaa
    35281 gtggcattcg tattcacgac ttgtcctagt aaaagttctg ttccgacagc tattatttct
    35341 gctttcatta cttcccgctc ccttatatag tatacgctgt atttttatat ttttttattt
    35401 aaaaagaggg caaggagctg tgacaaaaat cttgtcacag ctccttgttc caaataaaca
    35461 gtgttctcct gccaaaacct cggtcataag tcacaatata cataaaacat gaaaagctgt
    35521 tttttgaaat tatcccttgt gactcagagt tagacggcag gctcacaacc tctccactaa
    35581 acgatactta cattgatcct ttgaacacac cgctgttttt gatgaagtaa tccacacctg
    35641 aatagatagt aaagatcaga caagcataaa gcatgatttg atctactgga ataccgatca
    35701 ggctgaatgg gatattattg ataaacagca agataatagc aaccatttgt gtagccgtct
    35761 tgacttttcc cggccaagct gcagccatga cttctccatg ttcgacaagt agtaaacgca
    35821 agcctgtgac ggctaattca cgacagacaa tgatcgctac tacccaacca ggggcctttc
    35881 cttggtcaac caacatgata aaagcagtca taactagcat tttatctgcc aacgggtccg
    35941 caaacttgcc aaaattagtg acaaggcctc gcgcacgggc aatttttcca tctaaccagt
    36001 ctgtgatact agctaaagca aaaataactg cacctactag ctgtgtgaca gccagtgtgg
    36061 tgtctcctac gttaaattgt ccccaatcta aaggtaaacc tacaaccaaa atgaaaatcg
    36121 ggatcataca aattctaagt acagttaatt tatttggtaa attcacacta tcgactcctt
    36181 tcttccctat tctacgtgac tatcagcgaa gtttccaccg atattattta cttttccaaa
    36241 tgaatttatt caaaggagta tttttattga aattgatctt tttgaattaa cgaagaagga
    36301 ccgcgacaca gatttgtcac gatccttctt ttttatacct acaagtaaac agatgcgtaa
    36361 actaatttat acgatcattc tgcgtattga agagtaaggt tcacattttt tggactctga
    36421 ttgttttccc ctggattaac tggtacggat tctccattcg ctttgatcgt tgcattgctt
    36481 gctgcaccaa tcgtgatggt cgcatttgtt gctgtttccg gtaacacggt actctgtgtt
    36541 tcatttgctg ccagtgtccc ttgataaaca taggcattgt ctactagcac acctaaccat
    36601 acacgattga cagcagtaaa ttcaagggtt acaggcttgg tagcgtctgt gatatttact
    36661 gcgatggcac tgccagaatc ttctcctgta gctattgcca ttttcttttc ttcacttgat
    36721 tttgttgttg attctactgt agaagactct ttggtctgcg tgctttgctc tgaggaagag
    36781 gactcctcga ctgatccttc tactttaagc gaagaagctg tgtctccgat tattggatca
    36841 gcttgacgat cttgccaagt catatagccc acgacaacga caatcgccaa agcaaccaat
    36901 cctaataaaa tgacaggaag acttgtccaa aagcgacttg gatgcgattc ttcctgatgc
    36961 attgcttttc tagaagtact tactgtttca ggctgcggac gttcagggac cgatcctgtc
    37021 aatttgcttt tcccatcaaa aacatcgact aaatggtctc catcttctcc tacagcttca
    37081 gcgtattgcc gaataaatgc ccgcacgtaa aatttaccag gcaattggtc aaaatcatca
    37141 ttgtcgattg cttccaaata acgtttttga atttttgtaa tctgctgtaa ttcatctaaa
    37201 gatatttttt tattcaggcg tgcttgccgt aatttttctc cgatggtttc aaaagccact
    37261 cgttcatctt ctccagtctg tttgattctc cgaagcatct gctgttgcaa cgaaagacgt
    37321 tatgtttggg tgctgtgaca aaagtcatgt cacagctcct tttttccgaa taaacattgt
    37381 tctcctgcca actcctcggt cacaagtcac aatatccaat aaatataaga agctgttttt
    37441 tgaacttgtt ccttgtgact cggagttaaa cggcaggctc acgacctctt tcttaaacgg
    37501 tgttcaatca gctgttcctc ggtattagct caaaaaccgg caaaatataa ggagatattt
    37561 ttccgtatgt ttctctaata ctcaggtcaa aacgctgatt tcacaacctc ttccttaaac
    37621 ggtgttcaaa agcttgcatc tgcggtaata agtccaacca agttaaaaac attaagaagc
    37681 tgtttttact tggctgttct tattacttga tgcagggcag ctttttcaca acctcttggt
    37741 acacggtgtt ccagaagtag cttcttcggc aataagccga aatatcacaa aaatttgaga
    37801 agcaattttc gtaaattcct tcttatttct tgaagctgac cacttctgtc acaacctctt
    37861 tgattacttc gtttgatttt attttaatat aagttgatta aaagagagca tgatttgttt
    37921 tattttctca ctaatttttc caacacaact aagaatacca taaaagctga ttttggagga
    37981 ggttcctact acaagtctaa aacgaaattt aagacttttc ctcataaaat actggtttat
    38041 tttggtctca tgtaaaactc gctgaaggcc tcttcactta taaatgcttc cccagctgcc
    38101 agtacgtctt tcatttggat cgaatcgatg atttcaggaa gatcaaataa agttcgatcg
    38161 ccaaataaac tttgcgtaaa ttgattggca atatattcga tcgaatttaa tgattggaaa
    38221 tattgtccaa gcattttctt cttcagcaaa tcgagatttg tttctgacac ttccggatca
    38281 tcagcgaatc caagaatgat ttctttgact ttttctgctg ctttctctgg ttcgtccgta
    38341 tctccgctga agtccgcaaa gtgaaattca cgatccaagc tgaattcaaa accaaagctg
    38401 tcatcgataa ttccttgatt gtacatcgct agatagtttc gagaagtatt gcctaaaatc
    38461 atttggaaca acagattgat tgctgtttta tagcgtaaca gttctgttcc ttcttgcggc
    38521 aatgtgtcta atcctttaat accaagtaca aatttatctc gtgtaatggc tgcttccaat
    38581 gcactttgtt tgatgatttc ttttgtgttt tcagggaaat aacgtacaat ttcttgcttt
    38641 ggagggaaat tttttgcttc ttggttttcc cgaataagtt tcatcaattt ctcaggctcc
    38701 atcttaccaa ctacaaataa aaccatattg cttggctgat aaaacgtccg atagcacgta
    38761 tacaagtctt gagctgtgat tttgtcgata ctttcaaccg tgcccgcaat atcgatatgc
    38821 aatgggtgtg taggatacaa gttatttagt atgccaaaaa acatccgcca gttagggtcg
    38881 tcttcataca tttggatttc ctgaccgatg attccttttt ccttgtttac agtttcttct
    38941 gtgaagtaag gtgcttgtac aaaatcgatc agagtcgtca gattcttttc tacctgatcc
    39001 gtcgtagaaa acagatagct ggttttcgta aaacttgtga aagcattggc ggaagctcct
    39061 tgtttcccaa ataattgaaa gacatcacca tcttcttttt caaacaactt atgttctaaa
    39121 aaatgggcaa ttccatctgg aactttcacc ttttcttttt caccataggg aataaattcg
    39181 ttatcgatcg aaccataatt cgtactgaac aaaccatatg tcttgtgata gtcattttta
    39241 ggcaacaaat acacggtcaa accgtttggc agtacttcat gataaagtgt ttcattgatt
    39301 tgctcatatt ctgttttatt catttgcatt tcctcctgct aaaaagaaga ttgcctgcaa
    39361 ttcgatttgc tcagctactt gttgtatctc tttgatcgtt acgctctcca tacgtttcaa
    39421 ccattcttca tcagataatt ttgtttccgg cagccagctg tccaaaaaag aagcttcgat
    39481 tgccgcttgc ggagagtcta gagaaagcag atattggttt ctcagcattg cttttgtctg
    39541 agacagttcc aactctgtta tttcaccatt tcgcaaactt tctaattgct cgtgaatcag
    39601 atgaagtact tgatttcggt tcttttcatc gataccggtt tggacactca tgaatcctcg
    39661 gaacgtatcc acgctactag aggcataata agctagactt tctttctctc tgacattcat
    39721 gaaaagttta gagtgaggaa aaccgccaaa taaaccgtta aagaccatta gcgcaaaacg
    39781 ttctggttca tcgtaataca catttgtctg ataagctagg ttcagctttg cttgaacgat
    39841 tggctcacgt acttgtcgtt ccttcacgat attcacttga ggttgggtat aaaacagatc
    39901 tggataaact gtttcccgcg actcaaatgg taattgccca actgcttcag ctgctttgtc
    39961 cggatcaaca tcgccgacaa caaaaatgtc gatttgatca tttgccatca tttcttgata
    40021 gtaggacgct agcgaactag cagtgatctt gtccaaatct tcgactacgc ccaaccctgg
    40081 gatcttttgt tccgggctgt tttcaaaata aagttcttgc aaggcatagg aagcaaaagt
    40141 ttgtttatct tccttcaaac tttccaaata tagacgcaga ttgttctttt ccaagtcaaa
    40201 cgtcaactga tcaaactgat gatttttaat atttggataa aataaaacct cttttaaaaa
    40261 atctaccgct tgagaaaaaa ggtttggatc attgacatat tcaccattca caaaactgat
    40321 ccctgcgttt agccaatgta aatttccttt ttttctaact gataatccaa agcttgcgcc
    40381 ataaagctct gcaagttgcg cacttaactt tgtctgatcc gaataattca aactatttgt
    40441 ttccaacatg ctgctgagaa gtgaacgttt cgtgatgact tcttgttgca gacgcgcagt
    40501 aaaacggata aaaatccgga tcgttttgta ttgttttgtt ggtaagacat gaagattcac
    40561 gccttctgcc aattgataca ccatatttat atgctccttt tcacagttga ttgcccgctt
    40621 actcttttaa tgggtcaacc tgcctttttt acttattcct ctgcttgaaa tttataagtt
    40681 caggcaattc gccgtttttt ctaaatgatg ttacacttta tagtatagca tagatgtttt
    40741 ttatcaaaag aaacagacgg ctaaaagcgt atattgtttt tttatttttt atttttcgat
    40801 atactaaaga caaataaaag aagggagggt cattcatgat caaagaattc aaagagttca
    40861 tcatgcgtgg agatgtcttg gatcttgctg tcggcgttgt tattggtagt gcgtttacaa
    40921 gcatcgtaaa ccaagtggtg caaggcttga tcacaccact aataggttgg tttgtcgcaa
    40981 tgatcacagg tacttctgat ttagaaggag cactttcaat tcttgactgg tcgccgacaa
    41041 aaggcgtcac atttgcgttt ggtaacgtta ttagtgctat cattacattt ctgatcactg
    41101 gttttgtctt attcttagtt gtcaaagcgg caaaccgtgc gaagaattta cgctccaaag
    41161 aagagcaagt ggaagtggaa gaaactccta ctgcagaaga ttatcttagt gacatacgcg
    41221 atcttcttgc caaacaagtg gcagaagaca atacacatcc agtagagcaa acaaatcttt
    41281 ccgatacttc taaataataa agaatctaaa aaaaagaaag ccgattttca gtgtcggctt
    41341 tcttttttta gattcttaac ttgctacttg ttcctttttc atgtcgatat gtggaatatc
    41401 atcttccaaa tacacatctg agattgcctt gaatccaaac gactcataaa aagcttgtag
    41461 gtattcttgt gcgccgatca cgattggttt ttctggaaaa tttttctgga tcatgttaat
    41521 cgtttcttga acaatctttc ttcctaaacc atctttacgg ttgtcctgat gaacaaggac
    41581 acgtccaaaa tggatactct ccgcttcttc atatactcgc gtgtaggcaa ggatcttccc
    41641 atcagtatct tgaaaaaacg tatgccaagc ttctccatca atgtcatcaa cttcttgata
    41701 aggacactgt tgttccacaa caaaaactgc gattcgttct ttgacgatcg cgtaaaactc
    41761 tgctgttgac aattcttcaa atttctttac ctgatattcc actaagcaaa cgccccctgc
    41821 ttttttattc ttagcatacc gattcgcaca caaaaaagca aacgaacctt acgaatctca
    41881 ggctcgtttg ctttttaaac tgcttcagtt aaaaattcga tctcaactgt ccgagtaagt
    41941 acatctgaat agctgtaaga tactcgttca aaagaatttt cttcaggatc caaatcaaca
    42001 acgaatactg aaggataggt ttctgttaaa acgccgcggc gttctgtttg acgctttctt
    42061 cctgtctgag caaccaacat gattgggctg ccgatacgac cttctaaatc tttcttgatt
    42121 gaagctaaag ttgttggcat acagttcacc tctactatca actataccac atatttttaa
    42181 aattttcaag tttaccataa aaaaaaaaga aatgcaataa aaataaataa gaagctcatt
    42241 aagatttttc taaattattc ctcattataa aatttcaagt gtttttttat tttgagtttt
    42301 actctcccgt aagcgctcat tacttgcttg tgatccattg ctaaacattc agccatgtct
    42361 acaaaatcaa cgccttgcat ataataatga aaaattttag cttctatttt tgaaaactgg
    42421 atttcgcaat tttcgattgc ttcttttaat aaaagatgct gaattgctaa aggttcttgc
    42481 aatgtgatct ctgctaaaaa gtctggtcct tctgtctcta ttttttggtc gattgagact
    42541 tcatcttttt gatcacgacg cttcagcgcg ttctgcttcc tcaacagaga atagatttga
    42601 ttgcttataa cgcttttata ataacttcca aagctaacac ctttgctttt ttcgtaagtt
    42661 ctcaatgcct gatataaagc gatccttgct tcttgttccc aatcctcaga aacataattt
    42721 cttaaataat aggtgttttt caaacgtagt aaaagtggaa aatatttttg atacagggca
    42781 gcaaatgctt cttcgtcacc attacgtgca gcgcgcaata actcctcaga cataaaataa
    42841 ttcctccata aattcgttta tggaggaatc ttatcatcaa tgattcatcg aactgattag
    42901 aacgatatgt tatttatttt ttctcattaa atcgtccaat ttttgggaca atttttttaa
    42961 ctgctcttca ttccatggag aattacgtcg gaaatcaata aaatgcatat ctttattttc
    43021 ttgggctatt gttttttccg ttttttttat cgtcttatat aattcataag cagaagttcg
    43081 taatgctcct tgggaaaaga tcatccactg ttctgctaaa tcgcttgtcg ccactgtcac
    43141 ttgtgtcaaa cggtcattca gctgaccagc gattctctcg atataactgt cagccgtttc
    43201 gtcttctttc gtaaaaacaa catccaactg gtatttctta taattttgtt ggatgcccgg
    43261 aactagttgg gcatcgaaga caacgatgac ttccattcct tcatatttcg cataatttga
    43321 taaacgatgt aaaagagctt ctcttgcatc cgccattttg tcctgctttt taagctggac
    43381 aagttccggc cacgaaccaa tcatgttgta gccgtctaca atcaacagct gttttttcat
    43441 ggtctactcc ccctgacgtt gggaaaaagc ctgatacata agcaaaccag cagcaacact
    43501 ggcatttaag ctctggacgt gcccggtcat cgggatcgtc agcaattcat ccatttcttt
    43561 tttcaatcct ggactcattc ctttgccttc attaccaatg actaaagcaa ttgatccttt
    43621 ggcattccat ttttggaagt ttgtgccggt catatctgtg ccaaataccc aaaatcctgt
    43681 ttctttgagc tgttgcacag cttgtaccaa attcgtcacg cgtgcaacag ggacatgttc
    43741 gactgcccca gtggaagtct ttgttacgat aggtgtgatt ccaactgctc ggtgttttgg
    43801 tatgatcaca ccatcgacgc cacttgcgtc tgctgtccgt aaaatcgacc caaaattgtg
    43861 tggatcttcg atgctatcca aaatcaaaaa gaaggggtct tcttttttct cttttgttcg
    43921 ttcaatcagt tggtcaaggg ataaatattc ataaggtgta atcgcaagaa cgactccttg
    43981 atgcacgccg tgatcgctca aagtttctaa tttttgttta ggaacccatt tgactggcac
    44041 agcttgttca cgtgcgattt ctttcagttc ttctactttt tctcctcgtg aatcttcttg
    44101 aagaaagagt ttattccctc gcccttgttg tagcgcttct actgcggcat ggtgcccaaa
    44161 gacgaaatta tcttctaatg cttcttcatt ttgatcagaa actgactgat ttcgtccagt
    44221 ttgcttttta tttgaatatc tattttgttt tttgtcttca aatccacgtt tcgattgatg
    44281 tttgtttgtt ctttctttac ccattttttt caccaacttt ctgaatacac cagcggatca
    44341 attcttccat ccgctcggtt tgttttgtca aatgcagata gcccatgagc gcttcgaacc
    44401 ctgttgcgac ccgatacgtc gtaacatcag cattttttgc gctcgtatgg cttttagcat
    44461 ttcttcctct gcgataataa agttcttctt cttctgtcaa aagatgttct tccagcattt
    44521 gctgaatcaa aaaggcttgt gcattcgcag aaacataatt cgttgccgca cgatgcagct
    44581 tattgggttt tgtttctcct tgatcaatca ggtggtcacg aacatagatt tcgtaaatcg
    44641 catctccaac ataagccaag gctaaaccgt tcaattgtgt gtaatctctc atgcttctct
    44701 tctccatcgg gttccttgcg gtgtatcttc taataagatc cctttttctt tcaacaaatc
    44761 acgaatttcg tcgcttcgga caaaatcacg attgcttctt gcttgatttc gttcttcgat
    44821 caattgttca atctcttcat ctaataactc ttctgctgta aataaaatac cgaagatcga
    44881 aagccattgc gagaataaag cttctccttg tgccaagaca gcggcagaaa ctttcggctg
    44941 gtctagataa gtgttcagcc acttagcaag ctcatagacc actgtgatcc catttgctgc
    45001 attaaaatca tcgtccattt cttcgatgaa tcgctgttct aaattttcta acaaagcgat
    45061 tttatccgca tcatctgcca aattctctga ggcagtttct tggcggaatt tcaaattgtc
    45121 aaacgtattc ttcagtcgtt gtaaattggt ggctgcttct ttcatcgtcg tttcactgta
    45181 gcggatcgga cgacggtatt gtgtggtcgc catgaagaaa cgcaagactt gtggatcaac
    45241 gtttttgatc atttcatgta cagtgatgaa attccctaga gatttactca ttttttcatc
    45301 gtcttcgcca atcgttacat agccattgtg catccagtaa ttagcgaatt ttttccctgt
    45361 tttcgcttca ctttgtgcga tttcattttc atgatgagga aattctaaat cttgcccgcc
    45421 accatggata tcgatcgttt ctcctaaatg ttttgttgcc ataacggaac attcaatatg
    45481 ccaaccggga cgtcctttgc cccatggtga atcccaagaa atctcgccag gttttgcttg
    45541 tttccataaa gcgaaatcta atgggtcttc tttttgctgc tgttcttccc ctgtacgctg
    45601 acttgctccg acttccagtt catcgatgga ttggtcactt aatttcccat aattttcaaa
    45661 tttacgggta cggtaataca catcacctga agattcataa gcatagcctt tttcaaccaa
    45721 cacttgaata aatgctaaaa tatctggcat atgatccatg actcttggat gcagtgtagc
    45781 aggttcgaca ttcaacgctt tcgtatcttc ttcaaatgct cggatgaaac gatcagcaac
    45841 ttctggtgct gtaataccta attcgtttgc tgtacggatg attttatcgt ctacatccgt
    45901 gaaattcgat acgaaatcga ctttatagcc tcgatattca aaatagcgtc gaattgtatc
    45961 gaaagcaatg gtgctacgag catttccgat atggatataa ttatacacag tcggtccgca
    46021 gacatacata ccgacatgtc ccttttgtat agggatgaat tcttcttttt ttctggtcaa
    46081 tgtattgtag atttttatca ttctgatctc ctcacttttt ctcctttgat tcgaacgact
    46141 tttgctggta ctcctaccgc tgtagcgtcg tctggaacat ctttcaacac aacggcacct
    46201 gcaccgattt tggcgttttt tccgatcgtt accggtccta atatctgcgc attcgcagag
    46261 agcatggctc cttgtttaac agttgggtgt cttttacccg tttcttttcc tgtgccgcct
    46321 aatgttacgc catggaataa aatcacatcg tcctcgattt ctgcggtttc gccaatgacg
    46381 attcccattc catggtcgat gaacacgcca gttccaatcg ttgctcctgg atggatctca
    46441 acaccagtta ggaaacgcca gaattgagca ttgatcttcg ccaataaaaa gagccggtgc
    46501 tgatataaaa aatgagagaa acgatgccaa aacaaggcat gaaaaccagg gtatgtcaaa
    46561 acgatctcag ctgtcgaacg tgcagccgga tcgtttctct ttgctgcatc aatcgctcgg
    46621 gtaaaccaac tcatgacttt ccactccttt cgtaagaatt ttatgtagga aatcatcttc
    46681 ttataaagaa aagaagcgcc ttttaaataa gtttccttat caaaagacgc tttccgcgtg
    46741 gttccacttt tcttcataat caatgattat ctctgacagc taacgcctgt tatacgttct
    46801 tggctacttt gtttcctata aaacagtttt tcctgttttc tgtgtttcgc caagaccact
    46861 cccagaggca tttcggcaac cattcattga taccttgcac cacccggtat ctctcttgta
    46921 atgatcagct gcttactttt ttctgttcat cgtgtttagt tcagtacttg atttaaatgt
    46981 gctaatgatt tttcacgacc aagcaattcg atcgtttcag gcaattctgg accatgcatt
    47041 tgtcctgata cagctacacg gattggcatg aaaagatttt ttccttttac gccagtttct
    47101 ttttgaacag ctttgatggc agctttgaca gttggagcat ctactacttc catttgttct
    47161 agctgtgctt tgaatgcgtg gagaacagta ggaactgttt caccagcaag tacttctttt
    47221 gctgcttcat tcaatacagg atgttcattg aaaaatagcg tagacagatc aacgatttct
    47281 gcagcatagc tcatttgagg ttgatacagg ctaacgatct tcttcaccca ttcgattgtc
    47341 ttttcatctg ggttttctgc tacacgtcca tcagcgatta gataaggcag acacatgtca
    47401 gtaaatgcat ctagatccat tgctttgatg taatggttgt ttacccattg cagttttttc
    47461 gcatcgaaag ctgcgggtga tttacttagg cgttttggat cgaacatttt gatcaattct
    47521 tggtgttcga agatttcgtc ttcgcccaca ggtgaccagc ccaataatgc aataaagttg
    47581 aacattgcat ctggtagata gcctaattcg cgatattgtt cgataaattg caagattgat
    47641 tcgtcacgtt tgcttagttt tttacctgtt tcactgttga tgatcaatgt catatgtccg
    47701 aaggttggtg gtgtccagcc aaatgcttca tagatcatca gttgttttgg tgtattggca
    47761 atatggtcat ctccacgtaa aacgtgagtg attttcatca aatgatcatc gaccgcaaca
    47821 gcaaaattat aagttggcat accatcgcgt ttttggatga caaagtcgcc accgatatta
    47881 tcggattcga acgaaatagc gcctttcacc atatctgtaa atgcgtattc tgtattgcga
    47941 ggcacacgga aacgtacaac agattctaga ccttgtgctt ctttttctgc ttgttcttca
    48001 ggtgtcaagt tcgcacatgt tcctgcgtaa tgaggcattt ctcctcttgc gcgttgtgct
    48061 tcccgttctg cttctaattc ttcttctgtg caatagcatt tgtatgctcg attgctagca
    48121 agcagctgat caatcagcgg ttgatagatt tctttacgtt cagattgacg gtaagggccg
    48181 tattctccag gattttcagg agattcatcc caattcattc ccaaccaagc aaggttttcc
    48241 aactggcttt tttcgccatc ttcgatatta cgtttttggt cagtatcctc aatacgaata
    48301 ataaagtctc catcattgtg gcgggcaaac aaataattga ataatgcagt tcttgcattt
    48361 ccaatatgca ggtgcccagt tggacttggt gcatagcgta cgcgtacttt cgtcattttc
    48421 ttcctcttct ttcaaactag actttgacta acagcaaaaa ttccgtcagt tacggcttct
    48481 caaatatcat ttatttaaag ccactcagca caaattttac cttttctaag gtaaatagta
    48541 aagccaactg gttgaatttc cttaccagtt ggctgaattt cttttttatt ttgattgttt
    48601 agtctttttc tctgtctctt ttttattgtc gattcctcgt cctgcatgtg caggtttagc
    48661 aaagatcatg cgtccagcag ctgtttgaag ggcacttgtt acaacgactt cgatacgttc
    48721 attcatgtaa tgctgtccat cttctacgac aaccatcgtg ccgtcatcaa ggtaggctac
    48781 tccttgttgg cgttctgtcc cggcttttac tactagaaca ttcatcgttt caccagggat
    48841 gacgactggt tttacggcat ttgccaacgc attgatatta agaacaggta cattttggaa
    48901 ttcagagact ttattcaagt tatagtcatt ggttacaatg acaccatcta aaagcttagc
    48961 caatttgatc aatttgctgt ctacttcact gatatcttca aagtctccat cgtacatttc
    49021 gacagatata ccctcttctt tttgcaaggc atttaggata tctaatccgc gacgaccacg
    49081 cacacgtttc aaactatccc ctgagtccgc gatgtattgt agttcgtaaa gaacaaaatt
    49141 agggatcatc aaggttcctt ctaagaatcc agtctttgta atatcataaa tccgaccgtc
    49201 aatgatcacg cttgtatcca agattttata tttatggaaa ttttctcctg attttcgttc
    49261 taacagttga ctctcaactt gactagctgt ttcttgctcg atttttttgc ttcgtgaacc
    49321 aaaaactttc ttccattctt cgattcgtgt cgttcccatt ctcaatccca gatagcctaa
    49381 gatgatcatc agtaaaatcg gcaatacact attgacgaat ggaatttcca tattgtaaag
    49441 aggggtcgag atcacaacac ctaaagtcaa tccgatgatc gcaccgatcg caccgaagag
    49501 aagataagtc aagctgacct cacttagttt ttgttctact tgtttgataa tagctgagat
    49561 atgtttggcc aacagcaaag ataataaaaa gaaaataagt gcgcctaaca ggctgttggt
    49621 aaatgtatta ttcagccatc cgtttgactg ttgaccgatt gcctgccaag ctgagggtaa
    49681 aaatgaaata cccaagcttg cgccgacaat gatcatcagc aaagtaatga cacgtttttg
    49741 catcgttttt cctccgtttc gacaatcatt tattagttag cggaacacac gcttcaacgt
    49801 ttcagccaaa gttgctactc caactatctc gattcctttt ggcgcttccc agttacctaa
    49861 gttgttttta ggtaggtata cttttgtgaa gccgagtttt tggacttctt tgactcgttg
    49921 ttcaatatga ctgacacgtc tgatttctcc agtcagtcca atttcaccga tgaaacattc
    49981 cgttggctgt gttccttttt ctttatagct tgaagcaata ctgacagcga tagctaaatc
    50041 aatcgctggt tcatttaatt ttaccccacc tgcagctttt aagtaagcat cttgattctg
    50101 aagaagcaac ccagctcttt tttccagcac tgccatgatc agagataccc gattgaaatc
    50161 aagacctgtt gttgttcgtt tggcatttcc gaacatagta ggtgtgacta atgcttggat
    50221 ctctaccaaa attggacgtg tcccttccat tgccacaacg atagcagagc cagttgcacc
    50281 gtctaggcgt tcttctaaga aaatctgtga aggattagcg acttcttcca atccatgctc
    50341 atgcatttca aaaatcccaa tctcattcgt tgagccaaat cggtttttta ctgctcgcaa
    50401 aatccgaaaa ctatgatgtt gttcaccttc aaaatataac accgtatcca ccatatgttc
    50461 taacattctt gggccggcga tcgagccttc tttggtaaca tgaccgacga taaaaatggc
    50521 tatcccattt gttttagcgg ttttgagtag ttctgcagtg gtttctctta cttggctgac
    50581 acttcctgcc acacttgtga tgtctggctg cgtcatcgtt tggatggaat caatgataac
    50641 ataatcaggg gagatatgtt cgatggcacg actgatttcg ttcatatctg tttcagcgta
    50701 tagataaaat tctgtgttga tacttccgag gcgttccgcg cgtaatttta tctgctctgc
    50761 gctttcttcg ccggatacat ataagacttt tccgcctatg gctgctaatt gctgagaaac
    50821 ttgtaacagc aaagtggatt tcccgatacc tggatcgcca ccaatcaaga ccatagaccc
    50881 tggcaccaca cctccgccta acacacggtt cagttcttct aattttgttt tgatccgagg
    50941 ttctttttta gggacgacgt ctgcgatttt tgttggtttt gctttttctc ctgtcaaact
    51001 tgtccgagtt ctgcggtccg ttgtatcttg ttcgatttct tcgaccatcg tattccattt
    51061 tccgcaattt gggcagcgtc ccaagaactt aggcgacaca tacccgcaat tttggcaaac
    51121 aaattgtact tttgattttt ttgccattgt accgcctcct tttatcctaa tcatgagaca
    51181 ttaaatcaag cttaagtatc tttaacgttt tatttactgg aagaaccaaa tccgccttga
    51241 cgttgagcag tcgtttgatc ttgatctgct tttaaaaacg gcaaaaagat cccttggccg
    51301 atacgctcgc cttttttgat cactacatcc ttgaaaccaa agttggtgaa ttggaacatg
    51361 atatggcctt catttgcctc attattgtaa taatcactgt caatcacacc tactccgttt
    51421 gtcaacacta aaaaccgctt caacggattg cttgaacggt tagcaagctg caaaaattca
    51481 tcttctccca tgtaagcttt gattccagta ggcacgaggg ttggtttcaa ttgtttttca
    51541 ttttctgata catctttttt caatccatct gcgactgttt ccaacccctt tttccagcct
    51601 gctttccaga tacttggaat cacgacctct tctgctgctt caaaatcata gccagcagca
    51661 tgatgtgtag cgcgctgcgg tagattgata ttttgatctt tatatttaga aataacttca
    51721 aatcctcttt ttctcaccgg ttttcctccc taaataaact actcctattc taacatatcc
    51781 gaggctgtaa aagactttct ttacaaattc ttcaagaaat tctgctatct taaagtaaac
    51841 cattaattcg ttctttttcc aaacgactgc aagttgtaca aaaaaactat tttttacatg
    51901 actttactcg tcaaaacaag tagaatagaa cgaaattaat gagtggatat ttgattgagg
    51961 cgtcacatgc gtaaagaaat taaaaatgca aaacgaatta ttgtcaaagt aggaacaagt
    52021 acattgattt atcccaatgg aaatatcaat cttgcggcaa tcgatcagtt agcttttgtc
    52081 ctttctgatc ttagaaatcg gggaaaggaa gtgatcttag tctcatcagg agctgtaggc
    52141 gttggtatgc ataaattaca tttgtcagaa cgtccgactt tgattcctga acagcaagct
    52201 gcggcggctg ttggtcaagc cgaactgatg aatatctata atcaaagatt cagtgtatat
    52261 aatcaacaga cggcacagat cctcgtgaca agagacgtgg tagattatcc tgaaagtcgt
    52321 aaaaatgtga ggaatacatt ggaacagctt ttgattatgg acacgatccc tatcgtgaac
    52381 gaaaacgata ctgtagcagt ggatgaatta gatcatctga caaaatttgg cgataatgat
    52441 cagctgtcag ccatcatggc aaaacttgtc gcggcagacc ttttgatcgt cctttctgat
    52501 atcgacgggt tttattcaga taatccgact acaaatcccg aggctcagat gtatcgctat
    52561 atcgataaaa tcgatgaaaa gattctggca caagcaggtg gtgctggtag cacatttgga
    52621 acaggcggta tgcagagtaa attaaaagcc gcttctagga tatttgaagc aaacagcgca
    52681 atgattttag caaatggaaa aaatccggca attatttttg atattcttgc aggaaaagaa
    52741 gtaggtactt tatttaagga ggacttgaaa tgactgactt gatgcaatta ggacaacagg
    52801 caaaagatag cgcgcaacaa ctagcactta tgcctacaaa ccgcaaaaat gagctacttg
    52861 ttcaaatggc acaaaccatc aaaaagaatc aacaagcgat cattgaagca aatcataaag
    52921 acttagaaaa agcagtcgaa aacaatatca gtgaaacgat gcaagaccgc ttgcagctga
    52981 cacctgagcg aatcaatgcc atggctacag agatcgaaaa aattgcttcg ttagacgatc
    53041 ctattggaaa agtagatgag atgtggacga acaccgacgg attgagaatc ggcaaaaagc
    53101 gcgtgccact aggcgtgatc ggcatcattt acgaatctcg tccaaatgtc acgacagatg
    53161 cagctagtct agcattcaaa tcagggaatg ccgttatcct tcgcggtggc aaagaagcat
    53221 tcttctcgaa tcaattatta gtccaattat tgcagcaagt actggtcagt gctggcgaat
    53281 ctccttatgc catccagttt gtcgatgata cttctcatga aactgcagga cagttgatgc
    53341 gattgacaga atacttagat gtgctgattc ctcgaggcgg agcaaaatta atccaacgag
    53401 tcaaagaaca ggcaacggta cctatcattg aaacaggtac aggaaataat cacgtatata
    53461 tcgataaaga agcacagtta tctatggcag tgaacatcat cgtcaatgca aaagcttccc
    53521 gtccatctgt ctgtaacgcg gcagaaacat tgctgatcca ttctgagatc gcaccttttt
    53581 tcttgccagc tattgaaaaa gaactggtag aacacggggt ctctttacgt gcggatgctc
    53641 gtgcgttaga atatctagag acggctgctt tagcagaaga cacagattgg gatactgaat
    53701 atctagatta tatccttgct gtcaaagtgg tcgattcttt ggatgaagca attgcacata
    53761 tcaatcggca taatactaaa cattcagaaa cgatcgtgac agatagttat gcggctagtc
    53821 aacgtttctt aaatgaagtg gacgcggcgg ctgtctatgt caatgcctcc actcgtttta
    53881 cagacggatc ggtatttggt tttggtgcag agattggtat ctctactcaa aagttgcatg
    53941 cccgtggtcc aatggggtta aatgaattaa cttccacaaa atatatcatt tatggcgatg
    54001 gacaaatccg cgaataatgt ttcgtgtgaa acattattcc cgccttttgc cttttttctg
    54061 taaaggttct atccaactag aaaggtgtca atatgtttga aattttaatg atttttttta
    54121 tttaccttat ttctctgaat atcgctgctt ttctgggagt cggtatttta tcccttttct
    54181 ttcaattcaa aaaacgaagc atagggagtc aacgggaaaa atggtcgcag tattttgaca
    54241 aaatcgggca aaaagggctg gttacacgct tgcacattag ttacatggtt gcattatgct
    54301 tattagcgac aatcaattac tattcttttt tcgatcattc aatcgcttat acgattacgc
    54361 tactgattgc tgggattttt cacttatctt ataaatatca gctgaataaa aaccacttga
    54421 atcggacttt ccgctaaaaa atatccgtac gataagtaga gtgccttgaa gatcggcaaa
    54481 aaactgccgg actttttaga ggctgtgaca aagattttgt cacagcctct ttttccatat
    54541 aaacggcgtt cgcctaccag ctgcctggta aatctgtttc ctcttcgtta agaagcgaaa
    54601 atccgtcaaa tgtttcgcca tcataataac gaagcagctc atccatggac aagttcatcg
    54661 ttttttcttt ttcttcttcc gctgattcag ccagtagctg ttcccgatac acctgataca
    54721 taccagctat tttttgctgc atcgcttttc tagtttcacc ttccgcattg attattggca
    54781 attctttgat atagccacga taaatcaacc gatcatctga aaattcaatg ggtaattcaa
    54841 cgagtgtcaa cccgacttcc tgaaaatatt cttccagcat ctattatccc cccattcttt
    54901 ttcttaaatg aattttagct tacctactga aaattttcca gtttcattat tttgagaaaa
    54961 aaagggaatg tgacaaaaat cgtgtcacat cccctttttc cgaataaatg gtgttcttct
    55021 gccaactcct caatcataag tcacaatatt caacaaacat gagaaactgt tttttgaaat
    55081 tgttccttgt gattcagagt taaacgacag tctcacaacc tcttggtaaa cggcgttcaa
    55141 tcagctgacc ctcggtatta gctcaaaaaa cggtaaaata tgagaagcta tttttccgtt
    55201 ttgtttctct aatacttagg ataaaacgct gattttacaa cctctgttaa acggtgttca
    55261 atcagctgtc cttcggtatt agctcaaaaa acggcaaaat atgaggaact atttttccgt
    55321 ttgtttctct aatactcagg acaaaacgct gattttacaa cctcctgata aacggtgttc
    55381 aaaagcttgc atctgcggta ataagcccaa ccaaacgaaa aatatgagaa gctatttttg
    55441 tttggctgtt cttatgactt gatgcaggac agctttttca caacctctga tttcttattc
    55501 ttcttcattt gtagcaaaat aattacggtg ataaatgata caacatacac tgataagaac
    55561 aatggcacct gcaaaataaa acgcatagct ttcaccaata tgttcaccaa tcacacctgc
    55621 aataaatggt gctaaagctc caccgaaaaa gcggacaaaa ctatagctgg acgaagcaac
    55681 gtttcgttgc agacctggta tttccatagc aatcgtcgtc aacagtgtat tgataattcc
    55741 ttggaaaaat cctgctaaca caatgctgat cggtacaagg actggcatag aagttccagc
    55801 tcctaataac agcaaacaaa taatgaataa gacaaatgct gctaaaattg tccgataagt
    55861 cgtaaacttc cgctcaagag ccggtgcaac gaaaatcgaa gcaatagcca gaagcacacc
    55921 ccagccaaaa aatacaaaac cgacttctaa ttcagaatag ccaatcaata aaaatggcgc
    55981 atacgcaagt aaagtaaaga atccgaaatt atataacaat gcgattagtc caactgaacg
    56041 aagtttatga ttgcttaacg ctttgacacc ttcccaaagg cctacttttc tctttggttt
    56101 ctcagtttca ggtaaaagta ccgctagggc tataaaaccg caaaacatca aaaatgctac
    56161 cccaaaaaat ggataacgcc atgaaccact gcccaagacc ccaccgacta gagggccaac
    56221 agccattccg cagccaagtg ctgcttcata catcatgatt gctttttctg tgttaccaac
    56281 taaaatactg acgatcgttg ctaaagcagt agagacaaat aaagcattcc cgacacccca
    56341 gccggcacgt aacccaatca gcattccgat attcccagat aatccgccaa gaccagcgaa
    56401 tacaacgatg ataaacaatc ctgtcatcaa tgtttttttc aaaccgatcc gactagataa
    56461 gaaacctgta aatagcatca ccacaccagt gactaacatg taactagtaa ataacagggt
    56521 cgtttcagct ggcgtagcgt ttaactgtgc agcaatcgtt tttaagattg ggtctactag
    56581 acccactccc ataaaagcga tcatacaggt agctgctaca actagcgctg aacttttccc
    56641 ctttgtttct tcatttgttt gttcttgtat atctatttga ttctccaata tttccatccc
    56701 tttctctcta ttagcatcat acatgattta ttttacatgt caatatgcac atgtaaaacg
    56761 tggaaacttt actgtgatat aataaagaaa ataagacgag aggtaggatt tttatgaata
    56821 ctttagccta tatattatta tgcgtgcttt tatccaaacc acgttctggg tatgaattga
    56881 agcagctgat cacaattttt tgggaagccc atcacagtca gatctataca acgcttgcca
    56941 agttagacca gcagggttat gtgaccgttt tggacaaaga cgagcactct caaaaaaaga
    57001 tctaccactt gactgatgaa ggacgtgctc ttgtagagga atggatcaaa gaggaaactc
    57061 ccgctcctac gcaaaaagat gaatttctag caaaaatcta tgctttttcg acgctcgata
    57121 aaacaacagc tactggactg ctattcacta gagaacaaca gctgaacaaa atcatgcaaa
    57181 aaaatcaaaa aaaattgaat gacctctcgc tagacaagaa acaagaattt ggacgttatg
    57241 ttgtgatcca gcgcagaatt atgctttgtc agcaggaact ccaatggtgt cagcaagtaa
    57301 gagaacaaat gcagcagttt tttgagtaag ggataaacag aacctttcta gaaatatcta
    57361 ttgaagagca aattatcttc aataaaagag caatacagtt ggaaaagaaa aagcgatccg
    57421 aacgacaggc caaaacctat cgttcggatc actatttttc tagaactgca ttcaagagac
    57481 tatgacaaaa agcatgccaa ggcctttttt caaataacag tgttccatca ttcattactt
    57541 tgaattgctt ttagcacact tcttgaaagc gggccaacga tgatcaactg caacggtaaa
    57601 gcggcaatca cattcatttt ccaagcattc atataagcag agaataattg atcaggtatt
    57661 ccttgttcca tgatcacacc gaataaagac atacatgtca ccattcctaa aaccatcaag
    57721 catgaaatag ttaagatcat ttggaccttg ctttctggat ttactggtag tttaaaggca
    57781 atttttttcg ccagcacacc gatgatgaag acatccaaaa tgaaagccac cacgaatcca
    57841 ggcactagac caatcaacaa acttgataaa tgaagatctt gatgcagcca taaattatac
    57901 gcgctcatcc ccaagaccat caaaaaacac atcattgttg taaaaattat tccttctttt
    57961 ttatttgtag gcaatctgta ccactccttt aaatttgtca cgaaaatcat cgtagcacat
    58021 ttaaaaattt ctcgtaagta ctttttttat tttctgccct tttatttcat aaacttcatc
    58081 agccgtacgc tcgacaaagt aagaatcatg agaagtaaac aaaatcgttc ccggatatat
    58141 ttgcattagc ttctctaaag cttcgatcgt tgccatatca ataaaattag tcggttcatc
    58201 taacagcaac acattacttg gcttagtaaa aagcaaagca atcgtcagac gggtcgcttc
    58261 tcccccactt aatgtacata acggtttcgt caaagtttcc tgagcaaacc ctaagtgatt
    58321 cagcaaacta cggacaagcg attctgaaaa ctctgtttgt ctcatgacaa aacgaatgat
    58381 ggattcttca gatgtcattt gataaccctt ttgctgatat acttgaaagc ttactttcgg
    58441 agagagaagg attccttctc cttgtttgcg gatatgttct aataaggttg tcttacctga
    58501 accattttca ccgacaatcg cgattttttt acctaaagaa aactgaaaat caccatttac
    58561 cagtaatgtt ctcccacttc tttccaattg gacattttgt cccatgatgg gatagcggct
    58621 gtggagttca agagattttg gtactggaaa acggatttgt ttcatctgct gcggcttggt
    58681 tgtttctcct attttttgga gtcttctctc taatgttttc gcattctttt ggatggcttt
    58741 ttgtacactg tctttttgtt tagaggaaga caaccgatcc agtctgattt gttgcttttt
    58801 ctttttactg gacacttttt ccaacttttc cgcttgtttt ttcttttctt ggatcgattt
    58861 ttccaatcgt tttttctctt tcatgatcga ctctgcttct cgtagctgag tctttttctc
    58921 caattccttt tgagtgagat aggcactata attccccgaa aactctcgga tttctccgtc
    58981 ttttacttcc cagattttcg atgctagctc atctagaaaa tagcgatcat gactaacaaa
    59041 aaccagcgta ccataataat aacgaagctc ttcaatcagt cgatcaatac tttttctatc
    59101 tagatgggtt gttggctcgt ctagaagcat cccttgttca taagaagaaa gaagacgtat
    59161 caactcgatc tttcgttctt cccctccgct ccatttccgc tctgaagttt cttccagtct
    59221 taactggaaa tgactggcta attccaaatt taggtccttt gcttcagcga gataggttaa
    59281 ttgaggcagg tagttgaagg tgatctctgt ttggattttc ccatgatcgg gaaaaagttc
    59341 accggctatt agtttgagca aagtcgattt accgacacca ttttctccta tgatccctat
    59401 acgatcttgc tgatagacac gaagagaatc aatagatagt tctagctggt tgccaaagct
    59461 gacttgtaga tttgttatat ttactgctaa attttccata tgttttacac tcctatttca
    59521 tgtaaaacaa caattatcga tactaaaaaa gagcagaaaa atctgtttgc aaaaaaactg
    59581 catcacatct tttcctactc tgaacgaaat aactatgact aaatcaccaa atctgtcata
    59641 gctttcacct attctactgt cgttcgaaaa aatggacata ctccatccat gaaattgttg
    59701 tttatacatg tcttttgaat ttatttagtt gttattcaaa agttcgaaac gtaatttcat
    59761 cgatgcagtc atttttccga cctccttttt tattaggtta ccacacacga ttcaaaaaaa
    59821 gcaatagcta ctgaatagta actgaaaacc ttttattcga acatagtgat cttttctggt
    59881 gctaaacgaa cagactcgta accttcttct accgcttttc ctattgataa aataataact
    59941 ggaacataac ggtctttatc caaaccaaat gcctctgcca actgatctgc ttcgaatcca
    60001 ccaattggat ttgtttcata gccatgatca cgcgcaacta acatgaattg catagccgct
    60061 aagctagaat cgactttgac tacatcgttc atttcttctc ttgaaaaact cttgtaataa
    60121 ggaatgatcg cagctaattg ttgatctctg acttcttttg gcatcttccc tgattcgtaa
    60181 gcttggttgt agatttcttc gccatattcg taacattgca tatcgccaaa aatcatgacc
    60241 attgcagaag aagtatcatt ttgtctcgta ttgaaacgga tcaaaggacg caagatttct
    60301 tttgcttcct cgctttcagc gaccacaaag cgccatggtt tcatattgac agaagaaggt
    60361 gcagtcgtcg cttctttgat catttccagc atttcttcgt gtgggatttt gtatgtttca
    60421 tcatatacgc gaacggatct tctaccagtc ataattgttg aaaaatcatt tttttgttcc
    60481 attattatta acctctttcg tttctgttgt tatattgcaa agttgggtat tcaatttatc
    60541 taaaagtgtt agaaattgtt tcatttctac atcgctgaaa gccggggata gcacggaacc
    60601 tgtatcttta tggtccattt cacaatgctt gattttcttc tgggcttttt ctgttagcgc
    60661 tacatacact tcccggttgt tctgaggatt tctctgacgc gaaacatagc catttgcttc
    60721 caataccttc agatgacgag taactgctcc ttgatcaatc tgcaactttt cttgcaaact
    60781 gctttgtagc aatggatttt cttctttcag tctcatcatg atctcgtatc tagtaagact
    60841 tataccgata ttttcttcaa agatctggac gattttttga tcggctaatt tgatttgata
    60901 taaaaggtga ctaatctctt ctaaagacat aatttcctcc tttctaataa ttgactcgtc
    60961 aacgattgac ttgtcaataa tagaataaaa aaagaaaaaa gtcaaagaat cagctgtgta
    61021 ataaaaaaaa cgctctcaat agaaaagtat agagaaaaga aattttttgt agaatgaacg
    61081 gaaaaaatga taaagaaagc acaacgctaa aaccaagatt attgattctt atcaaactat
    61141 agaagatagc tcttctgcag tagataattt tacaccgcca aaaatttccg aaatcgcctt
    61201 ttgcaagaat cccgtcgaat attagaaatt ggatcagtat gcttaaacag taataaaaaa
    61261 gttatgacaa gatgttgtca caaccttttt tgagtaaacg gtgttcaaaa acttgcatct
    61321 gcggtaataa gttcaaccaa acaaaaaaca tgaaaatctg tttttgtttg gctgttctta
    61381 ctacttgatg cagggcagct ttttcactac ctctttttaa taaccataaa atcctattcc
    61441 ttcatatctc cagcccatcg gtgttagcca atctctttcc ttagcactca aggtatagtg
    61501 atgggtacca gttttttcat ttggattaaa tagtcgataa attggcttgg aaccaccaga
    61561 ataaaaactt agaccttcat aacgccaacc gatttttgtt aaagagttga tctcagattg
    61621 tgccattgtg taatgatggt ctccactgta aggattgtat aaacggtaga caggttgtcc
    61681 gctgtttggt gcttgccaag caatcccttc atattcccag cctacagttg gcagataatt
    61741 tttttctccg gcattcaatg tatataaatg ttctcctgta tgacggttat acagacgata
    61801 catcggagaa gttgaagttg gtgctgtatc atactgtgtc agattatagg tttcgattac
    61861 attgttcaat gtagaaccat aattaggcgc agtggcataa cgacctgtta gccaagccgt
    61921 agcatctttg taagagttcg tattgctttt ccatgcaccg gaatagtagt aaacgcctgg
    61981 ctgaaaagaa gtcgtcttca ataccgttac gttatcttgc aatgattctt tgtacgaagg
    62041 atattttcta aaagcggcat tcaccgtaat ccattgccca ttcacatatt ccgaagtagg
    62101 catcgtaaca gattggccat tatagcttcc tttaatccca aatagattgt aattaggggc
    62161 agaagccaaa gtactgttcc cccagccact ttcaacgatg gcttgagcaa tcatgactga
    62221 tgcatataag tcattggcac ttgccaaact ttgtgcagtt gaggcaatac tatcaatgaa
    62281 cgcttgttga gaattagcag atgagcgagc ataaaaagca tagccagtat agttttcgaa
    62341 ttcagacaaa tctgcttgat gggtatgtgt caatgtttgc ccatctgtca attggttcgt
    62401 gatttcttct gcattctctg gattgttctg agcttctgct gatgtactgt cttcggtttg
    62461 tgtttgttct gttgtttcag ttgttggttc ttcggttgaa attgtatttt ctgagacagt
    62521 agctgactgc aaagtacttt ctgtatcgtt ttcaattcct gtttctgaag aactcgatgt
    62581 ctgagttgcg acagtttctg tcgattcttc agctagcact gttgtgttgg caactgctag
    62641 atttcctaaa agaataacgc taaataataa attctttgct ttcatctttt ttctcctttg
    62701 aattatgaaa cttttcctaa ataacagtgt aactaaaaaa gtaaaagcaa gtcaatgtcc
    62761 atatttaaat aacatttgtt tctttttaat aaatttgctt tttctgcata aaaagacgta
    62821 aatgaagaat attcttctcc attcacgcct taaattgatg gtcgtttgaa taactattta
    62881 acaagtaatt ctctctggat attatccgtt atggattata cttcctgtgt cgtcggacgg
    62941 ataatcatgt tgctgacaga catccggtct ggcgtttcta tcgcaaatgc tacagcagaa
    63001 gctatatctt cagatgttaa tccccattct ttttgcgctt catgaagttc aagagcagcc
    63061 tttttgtcgc tgatggtttg atataattca gtttgaacag ctcctggaga gatgatcgta
    63121 gatttgatgt tattttgccg ttgctcttga cgtaatcctt ccatgattgc acggacagca
    63181 aattttgttc cgcaataaac ggcggaatca ggataaacaa catgtcccgc cacagaatca
    63241 gttgaaatga tatgcccaga tttttgtttt tccatgattg gaagtacggc ggaaattcca
    63301 tttaacacac ccatgatgtt gatatcaagc atattttgcc attcatcacg gtgaccttca
    63361 actaatggag cggttggcat gattcctgca ttattgtaca atacatcgat tcgaccgaac
    63421 ttttccatcg ctaaatcgat aactgcttgg acttgtgcaa aatctcttac atctgctgtt
    63481 tgaatatata gctcagcttc tggtaaagat tcttttattg cttttaggcg atcttctcgg
    63541 cgtgcagcta taactagttt agcccctttt tctgctaata gcttcgttgt tgcttcaccg
    63601 attccgcttg aagcgcccat gatgacaatc actttttctg ataaatcatt cattctattc
    63661 tcctcctgtt ttgaaaaatc tcttgcttat actcactatc tcttatttca tcttactgtt
    63721 caaataatcc gttcgacagg tttgacgatt ttggcaggga ttcctgcaac gatggtattg
    63781 tctgggacat ctttcgtaac agttgcattc gctgcaacaa tggcattctc gccaatcgtt
    63841 acgcctggca gaatagttgc tcctgctccg atccaagcat ttttcttgat gtggattggc
    63901 tttactgaca agcctcttcg ttcttttgga tcggttagat ggttcaccgt gatgaggttg
    63961 acacgaggac caatcaatac tgaatcttct aaacagatgc cacctaagtc aacgaacatc
    64021 acttgacgat tgataaagac atttttccct atactaatat gtttgccgaa atctgtataa
    64081 aaaggcagca gaacagtcac cgtatcatct actttgtcac taatgatatt ttctagccgt
    64141 ttgcggatat ctgctggttc atggtaccca gtattcagcg ctgctaaagg ctcttcgttt
    64201 tccgcttgga tctgatggat cacttcaaag agcggatccg ttactcgaat ctctttattg
    64261 acgatttgtt tttcgatgag gcttgtcatt ttgctccctc cttttatcct tactttataa
    64321 cggacggctt atgatgtcta atacttataa aacatactta actattcctt ttaactatac
    64381 ttagtggtat gcttttttaa aaacaggagg gaaaaaatgg atattcgtat actgacgtat
    64441 tttttagcag tagctagaga aaaaaatatc agccgagcag caaaatctct gcatatcacg
    64501 caacctactc tctcaaaaca actgaaagat ttagaagaag aacttggtgt gacacttttc
    64561 actcgaggct cccgagagat acagttgacg gattctggtc gatatcttta ttcaaaagga
    64621 aaagaaatct tgtcgttggt agaaaaaacg gcgaacaatt taacaaatga cgcaatcatc
    64681 tctggagaaa tctacattgg cggaggcgaa actaaagcaa tctctttcat ttctgatagc
    64741 cttcattcgt tgatcaaggc gcatcctgat atccagtttc atttatatag cgggaatgca
    64801 gatgatatca tagacaaact ggatagcgga ttacttgatt ttggactcgt aatcgaaccg
    64861 acaaacaaac aaaaatacca gtcatttcga ctgccagccc aagatcgttg gggacttttg
    64921 atacatcgga atcatccttt agcaaagcaa acagtcatcc atccgcagga actgaaaact
    64981 gttcctttga tcatttcccg gcaaacgtca gtggacagtc aactagctga atggctaggt
    65041 ttcagcatag atcagttatc gatcgtcgga acatataact tattgtacaa tgcttctttg
    65101 atggtcgata acgggacttt tggtgcatta gctctagatg ggatcatcaa tacttatggg
    65161 acggaaaaag tattcatccc actcgatccg ccattacaag cttcactgaa tctcatttgg
    65221 aaaaaagacc agcctctttc tcctgctgct cgtaaatttt tagatgtcct aacggagaaa
    65281 tacagttaag tagagttttt ttccttcgct ttcagctttt taacaacgaa taagttaagc
    65341 aaaagatcca atgattaaat aacataagtt tcttaatttg ttccttcctt ttatttctga
    65401 taattccagt aaattactat aattataaaa aaagaattga caaaacataa ctgtctatat
    65461 aaaattgatt acgttaacaa taattcgttt catgtgacta gatcatacta ggcatgaaaa
    65521 atctctgagg aacatggttt ccctaggagg tttttcgtgt ctatttttgt ttggccgcaa
    65581 tcaactggtc tagcaaacgt gaaaaccaaa tcttcaaaaa gggtgcgcgc atgaaaatca
    65641 aaaagatctt taatcaaaat gcagtattgg tagatgacgg tggacaggaa aaagtagcta
    65701 ttggaaaagg gattggattt gacagaaaaa gaaatgattt gatttttgat cgtgatgtcg
    65761 aacggatatt tgtcatggaa ccagaaggtc aaatcaagtt gcaaaattta cttaatcaga
    65821 ttgatgaacg ctttttattt gcagcagaaa cgatcattga tcatgcggaa actgttttga
    65881 tggaaaagtt gaatgaacat gtattgatcg ctttgaccga ccacattgct ttttctgcgg
    65941 agaacattaa taacggtatc gtcatccgca acaaactttt aagagaaatc gaagtgcttt
    66001 acagtgatga attcagtatt gctcaatggg cagtagagta tctgacaaaa gagcttggga
    66061 ttccatatac ttatgacgaa gcaggatata tcgcaatcca tattcacagc ggacgaagcg
    66121 gtcggaacaa taaccatcgc agtatccgtg aagtgacgat tgtatctgac atcatccatt
    66181 tagtggaatc agaattggaa acagatatcc attcagaacc attttcactg aattattcaa
    66241 gactggtgaa tcatttacga ctgcttctac aacgaaccca tgcgcaacaa tatgctgttt
    66301 tagacacgga aatcgtagaa atggtcaaac gaaaataccc agaaagctat aagatttcta
    66361 aaaaaatccg tgtgttgctg accaaagaat accagctcgc gatcacgaaa gaagaactgg
    66421 gttacttggc catccatatc gagcgtttaa gaagcgcaat cgtacaaaca aatgttaaca
    66481 atcatgagga ggaaaaaaat tgaaagcata tctacaacga atgggacgtt cccttcaact
    66541 tccagttgcg gtcttacctg ctgctgcttt actagtaggg atcggaaact ggtgggcatc
    66601 ttatagtaac gacattgtcg ctcatttttt acaagctggc ggtaacgcag ttttaggtca
    66661 attacctttg ctgtttgccg ttgggttagc actagggatg tcagtggaca aatcaggtgc
    66721 tgctgccttg gcaggtttgg tcgcatttga agttccagta aatgttttaa aaactgattc
    66781 agtagctact ttattgaata tcaaaccaga atcagttgat ccatcttttg ctcagatcag
    66841 taacgtattt attggtatta ttgccggatt gattgcggca gctctttata atcgtttcca
    66901 cgaagcaaaa ttaccgatgg ctttatcttt ctttagcggc aagcgtctag tgccaatctt
    66961 ggctgccttt gtcatgttgc tgatcagtgc agtgctactg ctcgtatggc ctccggttta
    67021 ctctgcttta gtttcttttg ctaaattcat tttgagttta ggctggggtg gtgcaggtct
    67081 ttatggcttc ttcaaccgtt tactgatccc tacaggattg catcaagcaa tgaactccgt
    67141 gttctggttt gacgtagcag gaatcaatga tattggtaaa tttttggctt ctgaaggaac
    67201 aaaaggcgta acaggaatgt atcaagctgg atttttccca atcatgatgt tcgggcttcc
    67261 agcaggagca tatgctattt acagaaatgc acgtccagaa aacaaagcaa aaacagcttc
    67321 cttgatgtta gcagcagctt ttgcttcatt ctttactggt gtaacagaac cacttgaatt
    67381 ctcatttatg ttcgttgctt ggccattata tgtcttgcat gcaattttca ctgggatttc
    67441 tttagcagtg agtgcattct tccattggac agcaggattt gcctttagtg ccggatttgt
    67501 cgattacttc ctatctttga aaaatccgat tgcgaatcag ccattgatgt tgatcgtcca
    67561 aggattagta acagcagtga tttactactt tggctttaac tttgctatca aaaaattcca
    67621 tctgatgact cctggccgcg aggaagcaga tctttcagat gacgatactg caacgactaa
    67681 ttcagacaat aaatatgcgg cacaagcaag caaaatctac gctgcattag gcggagccga
    67741 caatgtaact tcgatcgata actgtactac acgccttcgt ttgcaagtca aagatacagg
    67801 cctgatcgat caaaataaaa tcaaagccac tggcgtacct ggtatgaaga tcatcgacgg
    67861 aaaaaatgcg caagtcatcg taggtacaga agtacaattc gttgcagatg agatggcaaa
    67921 actacatggt ggtgctgcaa cacgacctgc acaaacaaac accgtggtta aaacagaaac
    67981 atttgccggt ggaacgatcg aacaagatat ttacgctatc gcaaatggga aactgattcc
    68041 aatcactgaa gtttcagatg atgtattcgc ggaaaaaatg atgggtgacg gctatgctgt
    68101 attaccagaa aatggagaaa tcttctctcc aatcgctggt acgatcacaa acattttccc
    68161 aacgaaacat gctgtcggaa tccaaacaga tgctggtatc gaagtcttgc ttcatatggg
    68221 catcaacact gttgacttaa aaggcgaacc attcacttta tatgtaaaag aaggtcaaaa
    68281 agtcgcaaga ggtcaattga ttgctttagt tgatcttgcc gctattcaat ctgcaggcaa
    68341 aaacacggat atggtcgttg tattcacaaa tggcgacaaa gtccaaagct tagaaatcga
    68401 acccgcacgt gatgtaaaag caaatgataa aatcggctct gtttccaaca aagcctgagc
    68461 catcgtcttc cccagacttt tttatcgata aacaaacagg agctgagaca aaaatcttgt
    68521 ctcagctcct ttttccgaat aaaaacggcg ttctcctgcc aactccgcgg taataagtcc
    68581 agccaaacgt aacatatgaa gagctatttt ttatttgact cttcttatta cttgatgcag
    68641 aacagctttt ttacaacctc tgataaacgg tgttcaaaaa ccagtttatc ggtatgaagt
    68701 caaatttatg caaaaatgtg gaaagccatt ttcacaaaat ttttcttcat actcaaaact
    68761 aatcggcttt ttcacaacct ctttatacat tattcctgat ccaatatttc ttttaatgct
    68821 gcttcaagag aagttaatgg ttttcccaat actttttcta aatcatcaga ggaaatagct
    68881 aactgatcct ttttaatatc atgttggatc gctaaaaaca tgtcagctgc ttcttgaggg
    68941 aatccggctt cttttagatt ttcgacaaac gctgcatcac ttgcttcaat aatttctaac
    69001 tttttacctg tcgcacgctc taatgccttg gctaattctt catagtcagt tagattgccg
    69061 gataattcta agatttctgg agagtcactt tctactagta cttttgctgc agcttctgca
    69121 tattcacgtt tcaataccca acctgttttt ccatttcctg cagcataaac gaattgacca
    69181 gtttttaaag cctgcccaat gataggtaat tcattctcta cataccaatt attccgtaaa
    69241 aatgtatggg aaatacctga atccttaatc attttttcag tgaattggtg atctggcgct
    69301 aatacactga tcgagtgatc ggcatctgca aaacttgtat aagcgatata agatacccca
    69361 gctgcttgtg ctgccttcac tacattctta tgttcggctt gacgatttcc tggaacgcca
    69421 gagacaaaaa gcaatcgatc tattcctttc aacgcttctt tcattgagtc taaatcatca
    69481 taatcaccaa tacgtatatt aaatccagct tctttcagtg cttctccttt ttcttcacta
    69541 cgtactagta catagatttc attttgtgga accatctttt ttaagaattc cagcgcataa
    69601 ccaccaaaac ctccagttgc tcctgttact aaataatgca tatttgccac tccctattct
    69661 ttttatttgt taagatttac agctgtacat ttataaatta cacctttatc ataaagagtg
    69721 tggtaagata agtcaagcaa aaaaaatgaa aggatgacaa ctgatgaagt attctctgca
    69781 atttagtgat gcaatccatg tacttgccta tattgaaatg tttcaagata cccgatcact
    69841 ttctagtgag atgattgcca gtagtatcga aacaaatgca gcaaatgtac gtaaaatcat
    69901 gagtcagctc aaaaaatccg gtttgatctt tacaaaaacc ggaaaaccgc agccttccct
    69961 tgccaaaagt ccagatcaga tttctttact agatgtctat cgcagcatcg aaggtaatac
    70021 acagcttatc caagtcgatc caaaaacaaa tccaaattgc gtcgttggtg cgaatatcca
    70081 agaagcttta gaagaaagct acgctcggct gcagaaaaag gcagaagaag aaatggctaa
    70141 gatcacactt gatagtctga tacataaaat agccgttttg gaaaaagaaa aaagaccaga
    70201 aaacacaggc ctcgtagaga gatttttata attctctcta ctgtgcctgc atttttctga
    70261 tcggaatcgt ctacataaat tcttttattc tccgggggac aaaagccttg tcatgcccct
    70321 ttttcctctc taatgctcag gtcaaagcgc tgaagtcatg acgtcttgat aaacggtgtt
    70381 caagtagctg accctcggca ttagttctga aaaatgcaaa atatggaaag ctatttccca
    70441 tttttcctct ctaatgctca ggtcaaagcg ctactttcac gacctctata ttatcatggt
    70501 ttattgacta ccggtatcca gatttctccc atatatttcc cgttggtaac tcccatttcg
    70561 actacagcat ttggaccccc aacataggcg taatcttcta cttctcttaa aacatgccca
    70621 aaggccgtat tcgtcaaatc atttcttaat tcttcaggcg attctgctaa agcgattaca
    70681 acaagatacg ttccttttgg gaatgggatg attcgggtag tgtttggaac atcctgactc
    70741 gtttgaacac cgacatagta catcattttc ccgttatagg gttcgttgac tgtaaagaaa
    70801 taatgatttt cagcagcttg tttcaatgca tctattgttc cattaccgat aactttttgc
    70861 tcaaatgctt gtttctctgc agccaaaccg acaaaatccg tattgctact cttcaattcc
    70921 acaccaatac ccgtcaccgt aaaatctggt ttttcttcga tcctatactt tgccatttct
    70981 cccacccttt ctattatatt aagttcatct tacataaaat taaagggaaa tgacacgatt
    71041 acgctctttt ttctagggat atttttattt tttaactatt tttttgcttt cactgcaatc
    71101 caaatctcac tgtaaacatt actgaaatcc tctccccatt ttgtaaaaga gatctcggga
    71161 gcttcgacta actcataatc tgaagaagga agccattctg catagatttt cccccaagta
    71221 tcttgtaaag tttggggaaa tggcccttta ttaggaaaaa tggcccaagt gtgttcttct
    71281 atagcaagca tttctaaatc agcgtacgga ttttcttttg tcgtagcaaa gccaatcatg
    71341 tgcatcaaac tgcccttttc ttccaagcgg gctccatcaa atccataaga aacgttgatt
    71401 acttgatttg gatataaatc tcctaactta tgcatctctt ccctttgagt ctccgtgatc
    71461 gtctgcgcta acttttgtat ttcttgattg actccttcaa atttgacagg aacttttttc
    71521 gccacacctg ccacataaaa ctgttctttc ttttcgatac gaaattccat cgattttcct
    71581 cctctgacat caataaaaaa tgtcaagcgt ggaaaagatt tttgtatgcc gtatctgctg
    71641 atttctgaag gcagatatcc tgaccaatca cgaaaagctc ttgaaaaacc ttcgactgtt
    71701 tcatatccat atctaaacgc cgtttctgtc actcttgatc catctaggag atcgcgatta
    71761 gccaaagcca atctacggtt tttgatatag tccattagcg acatccctgc aataaacgta
    71821 aagatgcgtt ttaattgata ttcagagatt cctgtatttt cggctattat tttataggaa
    71881 agtccttctg tcaaatgttc ttcgatgtaa tcgatcaatt ggttcagatc tttaagcata
    71941 tttatcttcc tccttgcttg cctttttatc atattatcaa agaaagtcaa ttacttgatg
    72001 aatcaggaac caatttagtc agatgtagaa aatgttgtat ggatctaaag aaaattctca
    72061 agcagcgttt cgtaacggat aattggtatt atctgcctat ctatgtttta cctttagaaa
    72121 aataaatttt ggggaagaca cattctaagt aaacaaagcg gtaaaaatat cttttattcc
    72181 cttagttaat ccgcttacta cctactgtac aaagcaaatt gttgttcaaa ctgctgatca
    72241 gaacgctgct tggatttttc tttgcgatac atcatacctc ttttatcaat tggaacaatt
    72301 atttatttct aacaaaatca cttcattttt gtcagataaa taaaaaacaa aaaaagttcc
    72361 atctttaaaa cacgctattc ttttactttt tctcacattc tctaagaaaa gaaaaggaga
    72421 atgaattttt gacaattcga gcaaaagtga ttatcaaata tgtaaaatca gctacttttt
    72481 tcttaaacgt ttctttatac taaatataaa taagtgtttc atgaaaggag aactagatga
    72541 ataattggct tgagtatttt cctgaaaacg ttttggaacg cggttacagc tatcatctgc
    72601 acggttttgt ccgtcatttg aactatacaa gcaagtactt gtccgcaaca gtttctggca
    72661 cggaagatta taaggtagtg atcacttggg atgaaaagac caacatgact tgtgattgtc
    72721 tatatgccat agaaggaaaa aaatgcaaac acatggctgc tgtcttattc gcttacgaag
    72781 aacgtccaat aaaaaaatcc aactactcac tttccgaact ctcgtccctt gtttcctccg
    72841 ctagttcctc tcttgtacgg gagcttttaa cagagattct tatagaacat ccgcaattta
    72901 tcgaacgctt caaagttaag atgccatttc atgcaataaa ttattcagat aaattaacaa
    72961 ctatcatcca taaatacgat catattatca agaaaaacaa aaacaggaaa acagcgaagt
    73021 tcatcatgga gatgcgaaag ttcatccaag aagcagttga atcgctcatt cagcaaaatg
    73081 cctatcttcc cgcattcgaa ttgatcaatg aagtgattgc tactctcgaa acattctatt
    73141 gggagcctga ggatgaacga acccttcttc tgatagagga ctgttattat ttatggaaag
    73201 aattattagc agaagcacct catgcagaaa aaaggcaaat gttctcttgg ttcgtctgcc
    73261 aagtcgatca tactgacgcc tcttattcaa aacgctactc cataaaaatc ctgaaagaag
    73321 actttcggga aaaagaattt tctaaccaaa agaaaaaaat agataaaaaa actaaagaaa
    73381 cggtaaagaa agacgatttc aatgaaaaat agcaagtaaa cgataacaac catcctaaat
    73441 ccacaagaaa tacagcagac cttgctttaa aaacacgaat catgcaaagg atgaagtaaa
    73501 gaagacttaa cgcgtaccca atattagtca tttgactatt aggtacgcgt tttgttttat
    73561 aagtcatgac aaatttcctt cgttgctggg tagattttcc ggcattgttg atagatttta
    73621 tgatactttt ctacattttg taagagaggt tccactgcat ctgtatattg gataaaattc
    73681 tgacaacaac tttcaggtgt gtcgaaccac tctaatcttt aaagataaat attaaaataa
    73741 cgattaataa tattaacagt ctttatt
//
LOCUS       DAZKLM010000003        68129 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3764
            SAMEA115706913-rid22582563.denovo.003, whole genome shotgun
            sequence.
ACCESSION   DAZKLM010000003 DAZKLM010000000
VERSION     DAZKLM010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706913
            Sequence Read Archive: ERR13196647
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 68129)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 68129)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287823.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287823.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,004
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,849
            CDSs (with protein)               :: 2,849
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 41 of 96
            Pseudo Genes (incomplete)         :: 52 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 15 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..68129
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706913-rid22582563.denovo.003"
                     /isolate="VRE3764"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(150..2336)
                     /locus_tag="ACG842_000147"
     CDS             complement(150..2336)
                     /locus_tag="ACG842_000147"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HFQ0246718.1"
                     /translation="MNEKEILRLQKELKRWENKYKKLQQVFRLQEEIIATYEAAYGEA
                     PGLIEEEVQVKESEGNSSFFKMMEKRSYNRFNQEQKAAITYDMKKHLRIIAGAGSGKT
                     QTICAKAVYLMTQKQVDEERILMITFTRNAANELKKRVDNFSQRKTAVHIGTFHSIFF
                     RIYNEICRKFPEVAMQGIQGDFSKDTAQKVNAVLQQLIRKYNLYIFDQYGEKTIASRL
                     DYWQNMNLSVEEMVQLVKEKYDSIDKNARQPISERLYGLLTELQEQKRSQQLLEFNDV
                     LQNLKSALENEEIQHYIGQKYDYLFIDEFQDTNPLQWEIVKRMTKENRIKLIVVGDDD
                     QSIYGFRGSEPAYIKNFEKEYPTKTLFLLTNYRSRAAIVQGANRLISYNKNDRIPKAM
                     IPAQKEAGVMEAYLFSDTKAESQWLIKQIQTFIQKNGKYKESIILYRSASQTQQLVQS
                     LLKTNIPFVLEADSPYEGIFGIKSFQHFYQKIVHWQTALNLQKKNQAYQQLLRQLMAD
                     CYLKKSECDQYFSPKYQNIAVADYILSISPNLKSKSAEIREFEEALRKTDQHQEIYSL
                     VQSYLHLPRIAKEIDQSDQEWIREEVKQHATFRRLQALSQETQATAKELKTRLIAYRQ
                     GKLDALCIQSIHKSKGLSYRNVFLIGCNEGSLPYNGATEKKVIDFNEIKAEPVTTIEE
                     ERRLFYVAMTRARNRLYLCVPQMKNSRKLKVSRFIKETGSRVKKGK"
     gene            2554..3237
                     /locus_tag="ACG842_000148"
     CDS             2554..3237
                     /locus_tag="ACG842_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YoaK family protein"
                     /protein_id="HFQ0246719.1"
                     /translation="MQTSFHEGRTIGLLLTFIGGAMDSYTYIQYGAFASAQTGNIILS
                     IIQAFDGEWLSVGKKVLSTLFFFLGILLTKYLIDYFRKKEKHFWRLFVLYYEAVIFFF
                     VSLTPVHEHPALVTILIAFTASIQWVAFDKINGRAYTNLFTTGNLKGVATNLYDYCIS
                     KEKADFDRFFHYLSVVLAFIAGAIVLVFCHHLFGAHSILIVSILFLIMALTQTFHLWQ
                     FYHTNRFTI"
     gene            complement(3318..4037)
                     /locus_tag="ACG842_000149"
     CDS             complement(3318..4037)
                     /locus_tag="ACG842_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HFQ0246720.1"
                     /translation="MPIIVIIVAGTIGAGKSTLTEMLAQDLETKPFYENVEDNEVLPL
                     FYSNPEKYTFLLQIFFLNKRFLAIKDAFSHDDNVLDRSIYEDSMLFHLNADLGRVSEV
                     EVKQYEGLLETMLKELEEISPQKKPDLLVYIRVSFETMLARIKKRGREYEQLEQDPEL
                     YSYYKELNRRYEEWYEQFDICPKIVIDGDKYDFVADPACGEQIVQEIKMRAKKMTEEA
                     DAQSIRYTTNNPKAKGIFTTK"
     gene            complement(4349..4555)
                     /locus_tag="ACG842_000150"
     CDS             complement(4349..4555)
                     /locus_tag="ACG842_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0246721.1"
                     /translation="MENEQLFEVGETYAFKSDLFESPIKGKIEKIYENSVMIRVMKCA
                     VEDNDTAHNLHWQLVVRKSSTIEE"
     gene            4800..5402
                     /locus_tag="ACG842_000151"
     CDS             4800..5402
                     /locus_tag="ACG842_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009372 - quorum sensing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HFQ0246722.1"
                     /translation="MKDKVVNKLLEALFSGDKSEEDMVYVQVKFVLEVLLNNLGKLAV
                     VLVFSLVTGSWAETGITFLSYICIRRYAYGLHSDSEFVCLLWTLLYLWGVPLVMKHLQ
                     LTISFPMMVFLLISCFLLLLRYGSRGTAINPIEPEKRPPLLKKAISMFLIFSLITLFF
                     SASYFSTYLLLGIVLEIATLLPITNYLMNFGGIFHEKNNH"
     gene            5543..6823
                     /locus_tag="ACG842_000152"
     CDS             5543..6823
                     /locus_tag="ACG842_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HFQ0246723.1"
                     /translation="MFLWSIALQYLIYTICILLIDHHIRYKYLFAGVILISCILIGWV
                     YSILGIFTALIAIGMLMLTFFFFSTSRQQLVYALPFGLLTSLLGDHLTSIGDFFVLKS
                     SVIEPGDSLQYFHIVLSAFLSALLAFCLKKSLETWFHTVDRRMIGTIGTLILFTYYII
                     IFYTRFSGETPDVLALNTSFFILYLCVGLIILVYYWKISNKKLADQLLEQQVRMQQDY
                     IKDLEKNYQDLREFKHDYQNLLFSLHSYISEGDLEGLRSYYDQTILPTRQMMNLFPAN
                     LSMLDNMKVPEIRSLLSLKLMMAQEKGLIVQLHFPEPIELDTKHTVNLVRMLGIILDN
                     AIEGASAAKKKKIELTILKKNHSVMIRVINTTTNTLPLNQLKQKGFSTKTNPKNEGLG
                     LFILDELTKTNPYLFLETSIENQRFSQTIYIQIK"
     gene            6836..7564
                     /locus_tag="ACG842_000153"
     CDS             6836..7564
                     /locus_tag="ACG842_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytR/AlgR family response regulator
                     transcription factor"
                     /protein_id="HFQ0246724.1"
                     /translation="MIPIYICEDDPKQLEVLSTVIKNRIMIENLDSYVHISTSDPEEL
                     LNAAKIRTSSFGIYFLDIELEDSEMDGVAIGKLIRELDPLGKIIYVTSHTEMSMKILK
                     SNIEPTDYIVKEDLYDLKENVEHILSKIFEDFQMSQIQKDIFKIEFNDEIKFLPIKDI
                     QYFSTSLGAPHKLEVHLTHAQLQFYEKIKEIEKMHRNFIRCHKSYVINTQNVRSINKK
                     TREVTLANGETIPASIRGLKKLIS"
     gene            complement(7633..9156)
                     /locus_tag="ACG842_000154"
     CDS             complement(7633..9156)
                     /locus_tag="ACG842_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HFQ0246725.1"
                     /translation="MKRLIGILAMLVLAGMLTACGASGKAEDSKEKLSVMTTFYPMYD
                     FTKAIVGDEGEVELLIPAGTDSHDYEPSAKDMAKIQDTDIFVYNDENMETWVPAIQKT
                     LQEGNVHTIKATEGMLLLPGSEEGHDHDHEHGEEGHTHELDPHVWLAPSLAIKQVANI
                     RDQLIEAYPEKQEVWTKNAAAYTEKLQALHQLYQETFKQAKQRSFVTQHAAFNYLALE
                     YGLNQVSIAGLSSSEEPSAARIAELKHFVKEHGINYIYFEENAKDSIARTLANEAGVS
                     LEVLNPLEGLTNEQIENGENYLSIMEANLEALKKTTETENPLEDTLTPKKEKSVYNGY
                     FEDEDVQDRSLSDWAGTWQTVDTYVEDGTFDPVFEYKEKLNQDKTAQEYKEYYTKGYQ
                     TDIASIKISEKEMAFTFKNGTTKRSNYRYVGKEILTYEAGNRGVRYLFEAEDKESGAF
                     RYIQFSDHTIAPADSEHFHLYAGNESQEVLLKEMENWPTFYPDTMDGREIAQEMLLH"
     gene            9408..10589
                     /locus_tag="ACG842_000155"
     CDS             9408..10589
                     /locus_tag="ACG842_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFQ0246726.1"
                     /translation="MKKETDTTNRLIRFLGGKTSYYVLGIVILLALIIYFFHQISFIF
                     KPVGIIIATILPPLLFALILYYLLEPVIKRLSKHLSRNLSVIIVYVIILFLLGLGSIW
                     LIPFLEEQAKTLISSLPDLFQDFQESLRQFLEKTPFADSFNQFFASIDDVTSDIAGFI
                     GNYWESGAQRISNIFSTVTAIFITLFTGPIVAFFLLRDPQKFYQSVLAIVPPAFRTDF
                     KNLTKIANQQLGSFLKGQIIASFILGAVYWVCFLLIGLEFASVLAISAGLLCIIPYIG
                     PFIAFFPGLIIAFQDSTFMAVKFVIVWFAVQLLHGDLVIPRVMGDRLQIHPITILIVL
                     LVMGDLMGIVGVIFGIPIYTLVKLLVTFIFRKFKQRYNKFYGDKGEYENSSFSEEDYF
                     K"
     gene            complement(10649..12277)
                     /gene="treC"
                     /locus_tag="ACG842_000156"
     CDS             complement(10649..12277)
                     /gene="treC"
                     /locus_tag="ACG842_000156"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008788 - alpha,alpha-phosphotrehalase
                     activity [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HFQ0246727.1"
                     /translation="MTFKDKVIYQIYPKSYKDSNGDGIGDLKGIKEKLPYLNDLGIDM
                     IWLNPIYPSPQKDNGYDISDYTAIDPIFGTMEEFEQLIAEAKKYRIEIMLDMVLNHVS
                     TEHEWFKKALAGDQHYQDFFILREEPTDWVSKFGGSAWSPFGDTGKYYLHLYDRTQAD
                     LNWRNQAVREELFKVVRFWMEKGVKGFRFDVINVIGKDEQLKNNKENDGKPEYTDKPI
                     THTYLKELNQATFGTDSTIITVGEMSSTTIENCILYTEPSREELSMVFNFHHLKVDYE
                     NGNKWSTPPFDFAELKRLFHTWGEKMSEGNGWNALFFNNHDQPRALNRFVAIDQYRKQ
                     GAEMLATMIHLNRGTPYIYMGEEIGMIDPDYSSISDYIDVESLNAYKLLLEQGFTEKE
                     AFRRIKAKSRDNSRTPMQWSDTEQAGFTTGKPWLKVGGKLEEINVEKELSSEDSILSY
                     YKKLIRLRKTYPIVAQGDYHAYEANHPEVYGFLRQFEGQQLLVLTNFYDKDTRIAIPE
                     GFTEANILIDNYSDTVLHQSLELKPYQAIALLKN"
     gene            complement(12728..15439)
                     /locus_tag="ACG842_000157"
     CDS             complement(12728..15439)
                     /locus_tag="ACG842_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HFQ0246728.1"
                     /translation="MKTRTYLKAGFREIRQSKGRFIAIILIVMLGTLLFVGVKTAGPV
                     MQKTMDHYVGTAGLSDLQIVSTGGLTQKDIAQAEKIPDAKVETGKQIYYSNTGKNEVI
                     QIFSYNKNNKQNKLQLTDGKLPEKPNQLVLDEKAEDEGYKIGDIYQIDSDELKEKEYT
                     ITGFVRSPLFINNLERGYANVGNGSVDYFVYLPESVFKTDIYSVLYLDFTNVKNLNTY
                     SKAYKDKMEQNQEKAEKYLQDRPEERLEELKKSANEELEPAQQKIADGKAQTTQARTR
                     LEDAKKQLAQQSATIQQLPEAQRTIAVQTLSQQEAQLAEQERQLVEKERELASAEEEL
                     NRNAEKIENLTKPSYLYNERSENVGFQEFGDLSDRIAAIANVFPVFFFFIAALITFTT
                     MTRMVEENRREIGTLKALGYTKVEIAKKYAIYASLASGIGIVLGTILGTNLLPRIIFE
                     LSNERYDIGSALIFYDWPPILQAAAAFFIAAFGAAMIVLFKDLRERPAALLQPKAPKP
                     GKRIFLEYITPLWSRLSFNQKISYRNLFRYKSRMLMAIIGIAGCAGLMVAGVGLKDSL
                     SSVSAKQFGPIIDYQAIVTLDSSTEDSRQKAADMLQKQEKVNEILAIHTQTVELRKKG
                     HASQSLTVIVPSKTADLDRFIHLTDLEENPRSLPKTGIAITQKVAELFDLSTGDHISL
                     YDEDQKEWKVEVKEIVKNYLGSFIYMSPEYYASVTDQSMMDNAFLLRTDKMTTKEEEM
                     LSEKLLATDAVTNTSFMSKQIETQEESMANLDSVVLIFVVLSGLLAFIVLYNLTNINI
                     SERVRELSTIKVLGFFDKEVTMYIVRENIIFTLLGIIGGFGVGYILTDFILQQASMEN
                     VIFPLVITWWAYVLSAGLTIVFTVIVMIVTHFKLKHIDMIDALKSNE"
     gene            complement(15449..16150)
                     /locus_tag="ACG842_000158"
     CDS             complement(15449..16150)
                     /locus_tag="ACG842_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0246729.1"
                     /translation="MKYVEVKNEYKRYKMGETTITANDDISFSIEKGELVIILGPSGA
                     GKSTILNILGGMDTPDEGQIIIDDTDIAQFSDKQLTAYRRTDVGFVFQFYNLVPNLTA
                     KENVELATEVSPDALDPVEVLRQVGLAHRLNNFPSQLSGGEQQRVSIARALAKNPKLL
                     LCDEPTGALDFETGKQVLKLLQNASRQHGNTVLIITHNSALAPIADRVIHINDAKVRS
                     VELNDHPSSIDEIVW"
     gene            16336..16935
                     /locus_tag="ACG842_000159"
     CDS             16336..16935
                     /locus_tag="ACG842_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HFQ0246730.1"
                     /translation="MNDLKENMIFDLSKTAFVAIDMQKEIVNNGTLSPHTAASILTNN
                     DLLVRTLKRTAALKVLVNVDISTFPYLSQQTDMGGMTASVPPEFTDLLLKDSLKDTDN
                     MLTITKYNPSAFFGTSLDLQLRRRGIETIILSGVATTNGVYATALDAFQHGYHIVLAE
                     DACSDRDKESHQLFIKKIFPKTARVRSTKQIIEAIQQSK"
     gene            17016..18029
                     /locus_tag="ACG842_000160"
     CDS             17016..18029
                     /locus_tag="ACG842_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HFQ0246731.1"
                     /translation="MVKKKITIKEVAKLSGVSITTVSLILNGNTEKFSPKTVQKVLAA
                     KEELGYQPDYFARQMITKETKTIGVLVPDITNPFFSTLMRGIEDILYKQNFVTILCNA
                     DSDHQKEIEYLAELTRRGVDGFIIATSAVSTDAINENLKKQGRPFIVLDQKKSEGFSD
                     AVRTDDFRGGYLAGMHLLSLGHQTIALVYPENPPENVHARIEGFKSALDVYQIPHDQL
                     ILLPTQFSKQGGYQITAELLDSAATGVFALNDELAFGLYRGLEEAGKSIPEDYSIIGY
                     DNIDMCEYIKPKLTTIAQPIFELGQTSAKLLLDRIQFPEKEWEEKRLPVRLEKRFSTA
                     PLK"
     gene            18137..19054
                     /gene="rbsK"
                     /locus_tag="ACG842_000161"
     CDS             18137..19054
                     /gene="rbsK"
                     /locus_tag="ACG842_000161"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004747 - ribokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006014 - D-ribose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HFQ0246732.1"
                     /translation="MNTVTVIGSINMDTTLRLTNMPKPGETMHAHEIFHAGGGKGANQ
                     AVAAKRSGARTSFIGGVGADSEGQQLLDLLTKENIDTSGIAEIQGATTGQAMIMVDAA
                     GENSILIHAGANNAFHEQEVLKNKQLITNSDFIIAQFESSLDATILAFSIAKDAGKTT
                     ILNPAPARETIPTELLEKTDIIIPNETETEIITGIRVTDHNSLVAAAEKLHELGIGTV
                     IITLGSAGAFYHTEKEHGIVPAFKVDAVDTTAAGDTFIGALSSTLQPDLSNLKEAILY
                     GNLASSVAVQSYGAQPSIPYREALEQSAV"
     gene            19082..19477
                     /gene="rbsD"
                     /locus_tag="ACG842_000162"
     CDS             19082..19477
                     /gene="rbsD"
                     /locus_tag="ACG842_000162"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016872 - intramolecular lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HFQ0246733.1"
                     /translation="MKKGKVINSDISRVIAQMGHFDKLSIGDAGMPVPFGTEKIDLAV
                     SNGLPSFLSVLENVLEELEVQSIELAEEIKTMNPEILEKIKLLLPDTPISFIPHEEMK
                     KELNNCHAFIRTGEMTPYANIILVSGVVF"
     gene            19477..20850
                     /locus_tag="ACG842_000163"
     CDS             19477..20850
                     /locus_tag="ACG842_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFQ0246734.1"
                     /translation="METTMNPEQMVLSEQIHMLIEQLGGKDNIDSVLPCATRLRIKLK
                     KQPVANAEKLKQQIPSIKYFILLDRYAEIIMDQGLAPAYKELTRQIDHSAPKEKLNPI
                     EKLFGLISEIFTPIIPLFAGSGILKGLVVLATTIGWLSETSGTYHILSAASNAVFYFL
                     PIFLAFSAAKTFKVNGYIAAVIAAALIEPNLTSLLSDSGKAATDFLGIPVVLMQYTST
                     VMPAILGIFFYSFVEKFLKKYIRENMQLVFVPLLSLVITVPLTLMVFGPAGVYLGEGL
                     AAAITWMMDINGPISGAIIAGGWNILVIFGIQWAVNPVMISNISAYGFDRIVPLTGAA
                     NFGMAGAALGVFLRSKRSKTRSISGSAFASILLAGVTEPTVYGIAIPLKKPFVAACIG
                     AAAGGAVMGFAQVKAIAFVFGSLTTLPAFISGTFFWYLAGLAVSLVVAMITTLVSGFD
                     EDLMSYE"
     gene            21207..23351
                     /locus_tag="ACG842_000164"
     CDS             21207..23351
                     /locus_tag="ACG842_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HFQ0246735.1"
                     /translation="MKNKKTIRLLAATWLIAPSVLSTNTVFAVDNQIADQTVTTDTTT
                     SSSATSETTDSFTEDPTGTTNSTTGSTEPTEIDPADPSENEKPAETYSLTIAPSLIGK
                     IQLTASTADSPEQQPLDITESGTVEGLLAETKVTYTITPAEGEQIASVAVNGAKEVNY
                     DAKTFTVGTNNLSITAEFKAGPDTSTEKPTDPSEEVPSTPDPETPNKPDVGNNNNNNG
                     SNNSNNNNNNSNSGNSSNQTNPSGSNNNGNTQREPNHPNNGSSNGGTNNASSRPNTAN
                     QSGIENPSSASSDFVVKSPIDAVLPTNTTNVQQAIVREAFKHLGKPYVWGAKGPNTFD
                     CSGLTYYVYMKATGHYIGGWTGEQQYAGTQIPVSQAQPGDLVFWGPSSGVTHHVGIYI
                     GNGQFIHAPQPGDKVRVTSISDFTPDFAVRVNLAGLPAASGSLTGGSSILDGLDGSFH
                     FSQNQTTDQFLKKIADDAQEIGQKEGIYASVMMAQAILESGSGNSLLSSEPNHNLFGI
                     KGSYKGSSVTFNTLEQDSSGQSYQIRAQFRKYPSYKESLEDYADLIKNGLTGNPDFYK
                     PTWKSETKDYKEATKYLEGRYATDRQYSQKLNAIIEAYDLTKYDEPKKEAATTEEANA
                     EILNMSKRFDVPIKWTTDGVPITGLEFSTIKRVNNASSIFRVASIWDLWNNFTARQIP
                     ETTTRTVKAATVPVLTLLSLDKSFWLDLPLKK"
     gene            complement(23403..24443)
                     /locus_tag="ACG842_000165"
     CDS             complement(23403..24443)
                     /locus_tag="ACG842_000165"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HFQ0246736.1"
                     /translation="MEINEQNFGENYHLITITNQNGTSLSVSDLGARVVHFQTIIDNE
                     KRELILGFDEAKEYLEKDPYIGASIGRMAGRITNGTFKLDDETFEVKTDPDTGHCLHG
                     GAPSFEAKQWHYTVINGENEASVIFYQTSPDMENGFPGTLDVEVRYTLTNDDTWRVTV
                     QGISDRKTVFNPTNHVYFNLTGDPAQSIDEHILWLNSRLFATLNKDTTPTGEVVSVSG
                     TAFDFQRPKKLGEVFASDFRQSMIVGGIDHPFLLKKQSGLLNAAELTSPDEKVSIQVK
                     TDASGIVIFTANFGEDGPEMRGNKLVNHGGITFETQELPGAERLESFGDVTLEPNQVR
                     EFVTEYKINIKK"
     gene            complement(24720..26522)
                     /locus_tag="ACG842_000166"
     CDS             complement(24720..26522)
                     /locus_tag="ACG842_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0246737.1"
                     /translation="MLWKLSLTGIKSRLRDYLVLFSGLVMASAIFYMFESMASNEAFL
                     KSNTIVAATVIIFRFGSVLLGIITFVYILYANSFLMTMRQKDYAMFMMLGAKGRKIAQ
                     MIFSETFLVGIAATLVGSAVGVGLTSIVHRLLVDQLDLNISHFTPFSIHGLLITVLFF
                     AVLFLLAAVVNAFSIVKKPILALIRADQTPTRIKQHKFLFFLEVVLGIAFLGCGYYMM
                     KHVGTYQVFGIGVALVTIVLGTYFIFHSVIIFFLSLLKKSEQISLKKLNNFTLSQLNF
                     RIREYTQMLSMVAMLFALALGALTVGLGFKNEIADLTENNSGYDLVMNNPRAEDQQKI
                     KELSPTLSASYTQKEDAGTIYYNASEFAAQPLIMIKHEDGTPPKITKEKVPAKKMNEL
                     TVQEELRSYELPEQQEKEIKVVSQSEFDQLNLPQSTLQLVQVKDFQANLKPIETLVAQ
                     NKMNNPSLQKVEYSNQKYEMYEVYNGLFSGFEFMGFFLGLAFLTMLASCLMFKILSGS
                     KSDIARYEMLEKIGTRRGLLKQSIRREVGVLFLAPGILGVVHVLFGLQMFGLFMQNPY
                     KDIWVPFTIFFVLYFIYYVLTTWLYTGIVLKKRL"
     gene            complement(26536..27288)
                     /locus_tag="ACG842_000167"
     CDS             complement(26536..27288)
                     /locus_tag="ACG842_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0246738.1"
                     /translation="MNKIVEVQNVTKVYGKNNEKKTQALSGISFDVEKGEFIGIMGAS
                     GSGKSTLLNILSTLDKPTDGHIRINQKDVTTLKGNQLADFRANEIGFIFQDFNLLESL
                     TAQENIAVPLSLQGVRPKEIKQRVQKIAERLSISHILESYPSEISGGQKQRVAAARAL
                     ITQPTILLGDEPTGALDSKSARDLLDTMDELNTKDHVSILLVTHDPFSASYCQRILFI
                     KDGGIHQEVKRGDQSRDSFYREILSILGNLEQ"
     gene            complement(27297..27659)
                     /locus_tag="ACG842_000168"
     CDS             complement(27297..27659)
                     /locus_tag="ACG842_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HFQ0246739.1"
                     /translation="MKKLLIGLLVFIVVVLAGVKIADYVVMGGEEYYVQITTDGTKTV
                     SKSDSGQETVNYRYILPGYTKEGKEKELDFKGQLTRPLRKGAYLKITWNKNKGVTSYE
                     EVEKKDVPKTALEKLIKG"
     gene            complement(27835..28707)
                     /locus_tag="ACG842_000169"
     CDS             complement(27835..28707)
                     /locus_tag="ACG842_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HFQ0246740.1"
                     /translation="MREKRAFYINGYAGIVGLVVLALVGLFFFYLGMWQAKVWALFLS
                     IFLWLITLLLLSSATVVSPNQAKVILFFGQYLGTIRENGFFLTIPLAQKMTVSLKVRN
                     FNSSVLKVNDLDGNPIEISAVVVFKVVDTAKALFDVAYYQDFVEIQSETAIRHIASQY
                     PYDTFNENDLTLRGNTTAVSDELQKELQERLAVAGVEVIETRLNHLAYATEIASAMLQ
                     RQQAKAILSARQTIVEGAVTITQMALEQIEDGQEINFTDDRKVQLINNLLVSIITDKG
                     TQPVINTGDMKDRK"
     gene            complement(28758..29246)
                     /locus_tag="ACG842_000170"
     CDS             complement(28758..29246)
                     /locus_tag="ACG842_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5348 domain-containing protein"
                     /protein_id="HFQ0246741.1"
                     /translation="MKVEEGQREKLKTEMERLHTYITQLSQTFYDPDKEKVMVNYPNN
                     SEGRQLEQVYHEVFKHLLTVKKELDYYSLPIIDTGILKYDGKKERFIFKSVREDLPLS
                     AGMDLEVLVEDYFTEEKHWVRTKLDYLPQAAGGTQASGWYITEDKELELEGVMARIRK
                     KN"
     gene            complement(29384..30175)
                     /locus_tag="ACG842_000171"
     CDS             complement(29384..30175)
                     /locus_tag="ACG842_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HFQ0246742.1"
                     /translation="MLTGGLLAGATYTLGAQKSLTWDNGPRLIDMVTETKKIKDANKI
                     VIHAENQTVSVIRGLDFEIKTTYDKTEKPEIKEKNGVLTVNAVKKSTGAIISLEDTSA
                     QITIVVPADHELSELTIEGNNSAIDINEVSIKKVNTKTHDGWVSFNEFTGGTITAKDE
                     LGSLRLDEANVDNLIVTGENSNFHLEDSLISKEGKVILNGGNLSLHEMENSGYTIQTS
                     GESYIEKDYQPITNTLLEQGQKKLKITAKDTSINITTDQEDNETD"
     gene            complement(30230..30838)
                     /locus_tag="ACG842_000172"
     CDS             complement(30230..30838)
                     /locus_tag="ACG842_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HFQ0246743.1"
                     /translation="MNEYLNEVVDHLQEIPEDDRFDLIQYYEEYFLDSGKTLDQIIDE
                     YGTPKQFALKLKISYFSEEEQAENELVKNPKRQIRLIWLIIIGLFASPVLIPLALGFI
                     LVIGGLLVALLALIFSIYVLCISILGVGLFMIISGISVFGQSILSGLFFIGVGLFATG
                     SAVFISPLLLKGTKGLFYVMMNFVKWMGRRFITKRDLHSAGV"
     gene            complement(30831..31148)
                     /locus_tag="ACG842_000173"
     CDS             complement(30831..31148)
                     /locus_tag="ACG842_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFQ0246744.1"
                     /translation="MHIQIPTVLLDGTVLAALSKEDMYGYALTKIVQESLSVSESTLY
                     PVLRRLKKEGYLETYDEPFEGRNRRYYSLTPMGSRHLEEIIDEWIVFRDSLDRLLEES
                     TNE"
     gene            complement(31304..32032)
                     /locus_tag="ACG842_000174"
     CDS             complement(31304..32032)
                     /locus_tag="ACG842_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HFQ0246745.1"
                     /translation="MPTGVLINVGSVLFGGLIGGLVGNKLSEHFKAQLTMVFGVCSMG
                     MGIYSIAPMKNMPAVIFALVIGTAIGLIVHLGNGINKGAALMQVPISKIFPSEKLGMT
                     HDEFISTLVTVIVLFCASGTGIYGSLDSGMTGDSTILISKSVLDFFTAAIFACNLGYV
                     VSVIAIPQFIIFYILFLLAKFIYPLTTPDMILDFKACGGFLMVATGFRMINVKMFPVA
                     DMIPAMIVIMPLSWMWTNWIMPLL"
     gene            32191..33072
                     /locus_tag="ACG842_000175"
     CDS             32191..33072
                     /locus_tag="ACG842_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFQ0246746.1"
                     /translation="MDIHKFEVFLDLAETMNFTKTADRQFTTQGNISKQILSLETELD
                     VKLFERAHRKIELTEAGSLLLPYVKNVVEQYHAMQEVLETYTKDNNLSLNILTIPTMI
                     NYKGFSKITEFLKRHPEFTVQLKEVESVELTYNDANEHSDTIHFARSFQAPPSSIEWL
                     PTETDDFVAVLPKNHPLASKKKLDLSELKKENFLLLGPTTNLYQPVLDLCREAGFEPK
                     ISYKGARIDLIINMIANDMGIAIVMEKTVKNLLKENTVILPISPTTESYLAFTRKVGE
                     HSLASDTFWEYLNQEEN"
     gene            complement(33309..34352)
                     /locus_tag="ACG842_000176"
     CDS             complement(33309..34352)
                     /locus_tag="ACG842_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFQ0246747.1"
                     /translation="MKKKLFSQLWIYFASIVFISILVTLLCFLGFIFLLSRHSISPAE
                     SAGKPTLYPLFVFAGFSMVVGTGISIFVGRRILHPISALGTNMSLVATGDFSIRMDEQ
                     QKVAEVQQLYKDFNVMVQELNSIETLRNDFVSSVSHEFKTPLATIQGYVQLLQAPNLS
                     DEERQIFLYRIIESITQLSQLTENILKLNKLENQRIQLEKKEYRLDEQIREVIVFFQP
                     KWEKEQLELDIELAAVNYTGNEEFLYQVWLNIMDNAIKYNQINGQIHIKLFETATEIV
                     LEVTDSGVGMNEETRDRMFEKFYQGDTSRQISGNGLGLSLVKKILELHDGRIDYSSIE
                     GVGTTAMIRLSKQ"
     gene            complement(34355..35023)
                     /locus_tag="ACG842_000177"
     CDS             complement(34355..35023)
                     /locus_tag="ACG842_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFQ0246748.1"
                     /translation="MNRILVVEDDPQLQLLYRSVLERVGFSVITALNGEDALKKLEAT
                     RIELIITDIMMPKMDGYDLLDSLRSTRFDTPVLMITAKADFEDKKRGFKLGADDYMTK
                     PIDVNEMILRVEALLRRSKINYSSSLIIGQTKLELDAYTVLQDQHSLILPQKEFLLLY
                     KLLSYPNKIFTRQQLMDDIWGLDTNTEERTIDVHIKRLRTRFEKTEDFQIITVRGLGY
                     KAVI"
     gene            35165..37501
                     /locus_tag="ACG842_000178"
     CDS             35165..37501
                     /locus_tag="ACG842_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HFQ0246749.1"
                     /translation="MLQLKDIKKYYKVGETTTKALDGVSVAFRQKEFVAILGPSGSGK
                     TTMLNVIGGLDNYDSGDMVINGKSTKDFKDSDWDAYRNNSIGFIFQSYNLIGHLGIIE
                     NVELGMTLSGVSKDEKRKRAEDALHRVGLTNHMHKKPNQLSGGQMQRVAIARALANDP
                     DILLCDEPTGALDTETSIQIMELIQELSKEKLVIMVTHNPELAHQYADRIIEFSDGKI
                     LTDSHPHIERPKDDQFNLRRTKMSFWTALKLSFNNIRTKKGRTFLTSFASSIGIIGIA
                     IVLSLSSGFQKQIDNTQAETMAKFPITISKVTTNQTRDDAGLGASKADYPDSKTITAK
                     VSEEDKAQHTNKIDQTYVDYVTDIDPNLSNNIGFTRTTGINLLRDVNGKVQPVSFSNQ
                     NPDTESLSLSSTMSAMTGVGVSSFPTQLDTSKENFLKDNYSLLAGSYPASATDVVLIV
                     DGNNNTNINALKNLGFDVKEDEKLDFDDIVGTTFKLVNNNTYYTKLPTGNFIPNTDYD
                     AMYQNASDELKISGILRVKSSSTMNLLSPGIAYSDQLTTQIVNENKESEIVKAQRDSG
                     VNVLTTEKVDENAKQTLLSYLGGDSLPSSIMIYPNNFEDKEKILDYLDDYNKGKSDED
                     KIIYTDLAGTMTELTGGLMDAITYVLIAFAGISLVTSMIMISIITYTSVIERTKEIGV
                     LKALGARKKDITRVFDAETCILGISSGILGVFIAWLATFPINSILYNMTDLKNVAQLN
                     PVHAIILVIVSTILTMLGGHLPARMAAKKDAAIALRAE"
     gene            complement(37616..37927)
                     /locus_tag="ACG842_000179"
     CDS             complement(37616..37927)
                     /locus_tag="ACG842_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0246750.1"
                     /translation="MKKMEESFPKAVKVENIANILKVTFENGEVKYVKSHWTEEITDA
                     LQFGKKGRGKRKNLLALSRNMWIGTEVTIEADGTVFINGKDRYTPEELWYKGKKSIPE
                     L"
     gene            38108..39583
                     /locus_tag="ACG842_000180"
     CDS             38108..39583
                     /locus_tag="ACG842_000180"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HFQ0246751.1"
                     /translation="MTDQPYDLRKVIEELKTLPGQYHETDIEIDPEAELSGVYRYIGA
                     GGTVKRPTQEGPAMTFNNIKGFPGTRVNIGTMASRKRVGEILHHDYRTLGRFLKDAVE
                     NPIKPVKVDKADAPAQEVVHLATDADFDIRNILPAPTNTPEDAGPYITMGVVLGSDPE
                     KTMSDVTIHRMVLEDKDTIGMYIMPGGRHIGHFQKQFEALNKPMPITINIGLDPAIAI
                     GTTFEPPTTPLGYNELWCAGALRNEPIQIVDGIAVDEVGIARAEFIIEAEIMPNETMQ
                     EDINTNSGKAMPEFPGYTGDANPAVQVVKVKAVTHRKDNPIMQTTIGPSEEHVSMAGI
                     PTEASILALVDKAIPGKVVNVYNPPAGGGKLMTIMQIRKENEADEGIQRQAAILALSA
                     FKELKTVILVDEDVDIFDMNDVVWTFNTRFQGDKDIIVIPGMRNHPLDPSELPEYNPG
                     SIRFRGISAKTIFDGTVPFDMKEHFVRAKFQEVEDWEKYLK"
     gene            39630..40214
                     /locus_tag="ACG842_000181"
     CDS             39630..40214
                     /locus_tag="ACG842_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HFQ0246752.1"
                     /translation="MEQLAQRKKKIVIGVSGASGTIYAINLVKKLKEYPMIETHGIIS
                     PWAKQNLKLESDLSLTEFESLFDYHYSNKDLGAAVASGSFLTDGMIIVPASMKTVAGI
                     SVGLGDNLIARAADVTMKEQRKLVIVPRETPLNAIHLENLTKLARLGVQIIPPIPAFY
                     NQPQTIQDLVNHHTMKLLDQFEIVNEHSKRWQGD"
     gene            40216..40635
                     /locus_tag="ACG842_000182"
     CDS             40216..40635
                     /locus_tag="ACG842_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid decarboxylase"
                     /protein_id="HFQ0246753.1"
                     /translation="MEKETIVEVTKLGFTIQAAVKVIGKDLLITLTGGDTPHIGTVTT
                     FSRDTEMQTVRFPSHDGRFHKDDVLAEKIAVIIKTAVPGSCTITSGIHVDHISNAQIN
                     ASFPMSEELGYKILDWLEKIDFHIKEPVYYKDGEKPL"
     gene            complement(40916..41200)
                     /locus_tag="ACG842_000183"
     CDS             complement(40916..41200)
                     /locus_tag="ACG842_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1905 domain-containing protein"
                     /protein_id="HFQ0246754.1"
                     /translation="MREIYSFESKIYASEVGKGGAYIIFPYDIRKEFGAGRVKVQATF
                     DNVPYEGSIVNMGVKNPDGSVCYILGLRKDIRKNIGKEIGDIVAVTVKQK"
     gene            complement(41552..42262)
                     /locus_tag="ACG842_000184"
     CDS             complement(41552..42262)
                     /locus_tag="ACG842_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4811 domain-containing protein"
                     /protein_id="HFQ0246755.1"
                     /translation="MIIGVLIVSLLAFALTNVFAKKTWQTFLSLIFGLIFVASLSLIV
                     ANLSNHFGMEKVTETKTEKIVSSADSQGADMLLYKALGNGKEKVYLYRTNEKQEKPKA
                     TGTDNETNKVEKTDGDAEKVTKTTYWEYKNDMYKFWFNIADNNHEYDSRVNTFKIPET
                     WVELSTDQAAKLAELVKKQQSTMESEAKAYVQDGMVKAMTENPKMSKAEQEQRTKALA
                     AEFQQQAFAKLVKEAKGE"
     gene            complement(42259..43731)
                     /locus_tag="ACG842_000185"
     CDS             complement(42259..43731)
                     /locus_tag="ACG842_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008269079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family MFS transporter"
                     /protein_id="HFQ0246756.1"
                     /translation="MSNNQPVDIYGKPYNRTLLVVVLLIGTFCTVLNQTLLTTAFPTL
                     MKAFDISASDVQWLTTGFLLVNGIMIPITAWLINKFSSRNLYLSAMTIFLIGTITCFT
                     APNFSTLLIGRLIQACGVGVSMPLLQNIMLSIFPPEKRGSAMGMSGIVIGLAPALGPT
                     LSGYIIDNYHWRDLFGMVLPIVVLVLVLAFFLMKSVIPLSNPSIDILSAILSTIGFGS
                     LLYGFSSVGDDGWGSLKVIGFLVVGVIFIALFSWRQLHLEHPFLELRVFKSSTFTIAA
                     VLAGVTNMAMVGAEMVVPLYIQNIRGESAFHSGLMLLPGALIMGAMMPVTGAIFDKYG
                     AKRLAITGMFILTAATAPFAFLTKTTPVLYIVILYAIRMFGISMVMMPVTTSGMNALP
                     NNLISHGTAVNNTFRQIASSIGTAILISVLTNVTKANIPSKSVLDNTPLAYKDQAINA
                     TLSGYHAAFIVAIIFGILGFIIAFLLKKKEPVLMKAGDNA"
     gene            43869..44342
                     /locus_tag="ACG842_000186"
     CDS             43869..44342
                     /locus_tag="ACG842_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFQ0246757.1"
                     /translation="MVGASLKRLLENDQLVVGISELSDIVGVSPRQLRYWEQKGFIRS
                     IEADANCPRKYRLPTVIKVELIKKYLDDGFTLSKAAEKAEIRQKKMHHVRKVFSKAIK
                     DIELIDERYTILQIGNFKEENAKMYIIHDNETDELTYKLLPVDEKADYEQLLKEL"
     gene            complement(44495..45046)
                     /locus_tag="ACG842_000187"
     CDS             complement(44495..45046)
                     /locus_tag="ACG842_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFQ0246758.1"
                     /translation="MARKNTLRYILLGLLSKKKMTGYELNQSFKNEIGEFWQAKHSQI
                     YPELAKMEEQGIIQHQVEITGEKLEKKVYELTEEGKEQLAEWIHTPTNELPVNRDEFV
                     LKLYFVKDVNDPALVEIIQQQRDLHEEKRLHLLERKKTIFPTEKEKHENYGHYLILAH
                     AINRETEYANWLTDVLEESNRKK"
     gene            45151..45681
                     /locus_tag="ACG842_000188"
     CDS             45151..45681
                     /locus_tag="ACG842_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenolic acid decarboxylase"
                     /protein_id="HFQ0246759.1"
                     /translation="MKEFKELEDFIGTHFIYTYDNGWEYEWYAKNDHTVDYRIHGGMV
                     KGRWVKDQEADIVKLTDGVFKITWTEPTGTDVALDFMPNEKKLHGMIFFPKWVEEHPE
                     ITVTFQNEHIAEMEAAREKYETYPKLLVPEFATITYMGDAGIDNEDVISEEPYDGMPD
                     DIRSGKYFDENYKRVK"
     gene            complement(46164..46607)
                     /locus_tag="ACG842_000189"
     CDS             complement(46164..46607)
                     /locus_tag="ACG842_000189"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0246760.1"
                     /translation="MIRTTTKLSAKELESVLSIWLHANLDSHEFIPSTYWENQVESVR
                     KALPDAVIYLAEIDQEIIGFAGVIDQFIAGIFVKREYRQQRIGTQLMKKIKETNKTLT
                     LTVYQKNQSAIHFYLSQGFTIQTQQKDEAVNEEELVMKWNQNADF"
     gene            complement(46623..47783)
                     /locus_tag="ACG842_000190"
     CDS             complement(46623..47783)
                     /locus_tag="ACG842_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFQ0246761.1"
                     /translation="MKQSKQKQSRRLLFRLFLLICIVVGLLIALYPFYVDSLNSFIDQ
                     KRIEQVQKRTAAEIEVQRKKMEEKNQRLTDQGFNPGTDPFNEKNRNESTTSSQLEEWL
                     IGSINIPKIQLNISLYDRLNGRILENGAGVLQGTSFPLGGNSTHSVISAHSGLPNRRL
                     FTELDRLENGDTFILTVLGEKLAYQVKNIQVVLPDDTSVLTISEGEDLVTLLTCTPYM
                     INTHRLLVTGHRIPYNESVKKEEGKGNQERTMRQWFILAGTIIAVVILLVFIGRLIYQ
                     YRLSKKVLDFSFIISDSSGKPVNGSSFILKHKKKTLTRNGVPFSVQSDHHGKVKLDQL
                     PGGTYRIVSADDPKVAVSFGIRKVKQEKIYFFEGRKLVKELQKNGFYFKINE"
     gene            48290..49990
                     /locus_tag="ACG842_000191"
     CDS             48290..49990
                     /locus_tag="ACG842_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0246762.1"
                     /translation="MMNLSKVCKGVRQLKKYGPFLAIFFIALAFLMPHLFTHKLILGA
                     DAPFHYNRFYDAAQQIKYGDFHYFPSLYGFQQSGRIVNAVYGPFFAYLQGLIVLLAGS
                     WFHYQILSNFILYLISGFSMLILLRYVNVRNWVSVPVAVLFMSTYSIQYWSINQGFTS
                     WGTCFYPLCMIPIADFVLHKKFPVIKVAVSVGLMTQIHLLSSIMLALMYIPFYLYYFF
                     NQTEKGKALLQLIKSVLLYFVLTANIWAGLAIVYSGNKILPPFVNREMSQKAIDLDGS
                     YWLHYPKAFPILILSGFLLFIFFQQKNTLFQKIVLYTSLVFFFLSTSLIPWTTLIEKN
                     VPFISLIQFPFRFFVPFTLLLLLYLALSLNQWSEQKWFRLLSIFGILIFSAQTIQNLY
                     QHLEKWENETFVSRHTYLFDTPEEARKTFFSKDMSTSLFVFQKTTPDYLPIYKETKDN
                     KYDRYNEEILENETTFIKTHPGGLLTIKWKNTGDKTVHIPVIVYDRTVLQQNGKTLTD
                     YEVTDIGTPIVKQQKGINELTLHYQTPIYFYFILSLTLIGWFTLLCLFIYHRYKLLRA
                     "
     gene            complement(50092..50925)
                     /locus_tag="ACG842_000192"
     CDS             complement(50092..50925)
                     /locus_tag="ACG842_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HFQ0246763.1"
                     /translation="MKFMTLNTHSWLEPEPEKKLQELADKILLEDYEVIALQEINQLL
                     ESEEVEPAKLMKFCPVKNQVPIRKDNFAYRLVQLLKEHGKEYFWSWEMSHIGYDKYEE
                     GNALLTKKTLESQVLTVSQFQKKEDYQTRKILIGKTKIDDQDIFVSSCHFSWWTDKKS
                     GFYFEWKNLENYFLETRVPLFFLGDFNNPVDSQGYYTVRESCLLLQDSYVVANEKGKA
                     ATVEKKIDGWEQNTEKLRIDFIFVPEGMQVKKYQRIFDGIDSPIISDHYGVEIELDVN
                     E"
     gene            complement(51229..53394)
                     /locus_tag="ACG842_000193"
     CDS             complement(51229..53394)
                     /locus_tag="ACG842_000193"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015144 - carbohydrate transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIBC"
                     /protein_id="HFQ0246764.1"
                     /translation="MKKIFSFEFWQKFGKALMVVVAVMPAAGLMISIGKSIPLINPDW
                     TPLVTTGGVIENIGWAIIGNLHILFALAIGGSWAKERAGGAFAAGISFILINRITGAI
                     FGVTSDMLANEDAFTHTLFGTKIMVKGFFTSVLEAPALNMGVFVGIIAGFVGAMAYNK
                     YYNYRKLPDALSFFNGKRFVPFVVILWSTIVALILAVVWPNVQAGINNFGLWIAESQE
                     SAPILAPFLFGTLERLLLPFGLHHMLTIPINYTQLGGSYEILSGAQAGTQVFGQDPLW
                     LAWATDLVNLKGAGDMSQYEFVLHNWTPARFKVGQMIGSSGILMGMAFAMYRNVDPDK
                     KARYKSMYFSAALAVFLTGVTEPLEFMFMFAAVPLYVIYSVIQGAAFAMADILPLRVH
                     SFGNIELLTRTPLAIKAGLGGDLINFVLCVIAFGVVTYFLANFLIKKFNFATPGRNGN
                     YDNDSEETVVSNSGTGTADQQVVQIIHLLGGKENIKDVDACMTRLRVSVNDREKVGTE
                     EAWKRAGAMGLIVKDNGVQAVYGPKADVLKSDIEDLLQSGAEIPEPEMVSTGNGQAGS
                     KQYLGIEQELVSAASGEVIPLSEVKDPVFSQKMMGDGFAVIPTSREVVAPIAGKVTSI
                     FPSKHAIGLETKDGIEVLIHMGIDTVQMNQPAFEIAVKEGQEVGTGTKLAEMDLDVIQ
                     NEEKDPTIMIVFTNDKVEEVVIKQLGTVTAGKVIGSIKM"
     gene            53654..55948
                     /locus_tag="ACG842_000194"
     CDS             53654..55948
                     /locus_tag="ACG842_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HFQ0246765.1"
                     /translation="MKQIKRLFAIDPWKISTTQLDKKNLRLQESLTSIGNGYMGMRGN
                     FEETYSGDHHQGTYLAGVWYPDKTRVGWWKNGYPEYFGKVINALNFIAIDISINDQLI
                     DLASLEPEDFYWELDMKNGVLSRTFTITTSTNKVRISFERFLSIVKKESAYIYLTIEM
                     LEGSGTVKLVSKLDGNVQNEDSNYDEHFWNEIDRGTIEEIGYLTTKTIPNNFGIEQFT
                     VTAAMRHFVNGKSVIPDYHEDTLALHGCLSAELSQGETWSVEKEIIVLTSRDIPEAQQ
                     AEKAVSELTNQTNNYIQAKEEQTAAWAERWKLADVVIEGDEEAQQGIRFNLFQLFSTY
                     YGEDERLNIGPKGFTGEKYGGATYWDTEAYAVPLYLALAKPEVTKNLLKYRHNQLPQA
                     IHNAQQQGLKGALYPMVTFTGVECHNEWEITFEEIHRNGAIAYAIYNYTNYTGDTEYL
                     KNEGLEVLAEIARFWADRVHFSKRANKYMIHGVTGPNEYENNINNNWYTNTIAAWVLR
                     YTRENYLTYQKEAAITISEEELAKWKDIAENMYFPVDEELGIFVQHDTFLDKDLMPVS
                     DIPTSDLPLNQHWSWDKILRSCFIKQADVLQGIYFFNDTFTKEEKQKNFDFYEPMTVH
                     ESSLSPSIHSILAAELGMESKAVEMYQRTARLDLDNYNNDTEDGLHITSMTGSWLSIV
                     QGFAQMKTAHDQLRFAPFLPKTWKAYSFHINYRGRLLFISVTDEQVTFELLEGSPLEI
                     YVYEQSYSLADRLEITLRKEEANV"
     gene            55941..56606
                     /gene="pgmB"
                     /locus_tag="ACG842_000195"
     CDS             55941..56606
                     /gene="pgmB"
                     /locus_tag="ACG842_000195"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008801 - beta-phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HFQ0246766.1"
                     /translation="MFKGVLFDLDGVITDTAEFHYRAWRKLGQEIGITIDHTFNEQLK
                     GVSREDSLSLLLAYGGKEHSFSKEEFAELAKRKNDYYLEMIQTIEPKDVFPGVVPLLD
                     SLKEEKIKIALASASKNGPFLLEKMGLTPYFDAIADPAEATNGKPAPDIFLLAAKAVG
                     LKAEECIGIEDAQAGIQAILSSGAQPIGVGRAEDLGEEIPLVPDTTFLTIDYLKEKWH
                     DHG"
     gene            56599..57606
                     /locus_tag="ACG842_000196"
     CDS             56599..57606
                     /locus_tag="ACG842_000196"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HFQ0246767.1"
                     /translation="MDNPEISVVPVPETEIKKITLKNDYLEVVLLNYGARLHQIFAPD
                     KEGKSENVLLSYDRFEDVLTDKSFFGATVGPVAGRIRDASWGEHRLEKNCGSHHIHGG
                     TKGWSFQFWEVEVFKTPSSIGVVFYLKDEFSTYPGPITATITYRLTKNELEMTTSASS
                     LVETICNPTNHAYFNLSGNGTRDIYEHQLTVFLDGVLELDQEKLPTGNWTKKEKLPID
                     FRKSPTLQEILACYPDGLDDVFLLHHPRLSKTSLQLFEKHSGRQMTIATSNKSMVLFS
                     TTGFEADFSVNGKQMHSNYGLAIEPQEIPDIVHFPKFGSINLHPGQERISQTIYRFSA
                     Q"
     gene            57628..58647
                     /locus_tag="ACG842_000197"
     CDS             57628..58647
                     /locus_tag="ACG842_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFQ0246768.1"
                     /translation="MAITVKDVAKKAGVATSTVSRVINDHPSISETTKKKVRKIMDEL
                     GYVPNMTARNLGKRISSAIGVILPPLDSKERIGNPFYLEIMEAINEEARNYDMTTAIA
                     TAKNFELLLENVQRMHLQKQVDGFILVYSDSQDTVIDYLYQNHIPFTLIGQPYHHENE
                     IVYVDNDNQLLGKQATEFLIQNGHKNILFVTNTTHESLYFERYFGYQKAMMMAGLPAL
                     SSVVLEHPEDYLSFEKTLKENEATALVVIDDIFALRTMQLAQMYGYVIPETLSVISFN
                     NSIFSTLTHPYLTSIDIDISELGRVAMQKLRELIHEELSSGVRLVIPHRLIKRETVVP
                     LENPY"
     gene            complement(58928..60295)
                     /gene="nhaC"
                     /locus_tag="ACG842_000198"
     CDS             complement(58928..60295)
                     /gene="nhaC"
                     /locus_tag="ACG842_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HFQ0246769.1"
                     /translation="MNKNISLKESIAVLIVLLALLGVLIIGFQLSPHIPILAAFMCLL
                     FYGRFKKFTWDEIHDGIIKGITPGIIPILIFLLIGVLVASWILSGTIPTIMVYGFKLV
                     SVRFFLPTAFLVCAIVGVTVGSSFTTISTIGIAFLGIGHLLGFNDAMTTGAVVSGAFL
                     GNNCSPLSDTTNLAAGIGGVNLFEHIAGMRYTDFPAFVLSLIFYTILGHNSHSADLAS
                     INEMIQNMKASFWISPWTLIPLVILFGGAIKKIPAIPTLLAGSTAALVLSFIHEKSLT
                     VGKAADILMNGYVANTPDPKLDELLSRGGIMSMLGSASLILLALALGGLLIKYLIVDT
                     IVQELKEKMDRPSRLVGFTALSCIGINLIVGEQYLSIILPGETFKRSFDDVGLSKTYL
                     TRTLADSGATVNSLVPWGVSGTFIMGTMKVSALHYLPFAFFSILAPVFTILGGFLLNR
                     RRVKN"
     gene            complement(60404..61825)
                     /gene="tyrP"
                     /locus_tag="ACG842_000199"
     CDS             complement(60404..61825)
                     /gene="tyrP"
                     /locus_tag="ACG842_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070908 - tyrosine:tyramine antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-tyramine antiporter"
                     /protein_id="HFQ0246770.1"
                     /translation="MSKSEVSKMGLGTFIGLTMALCATVRSIPTLAAVGWTLIFYSIF
                     AVLFFAGPISMVSGELSTMLPQEGGPQLWVKTALGSKWGFVVAWLLWVQMFPGMVMVA
                     STLGPLLGNTFGNVELGNNHWFVLGCILVIYWIITILNLKFDMAKVGGNIGVWLGVYI
                     PVVIMFVLGVLAAFKVGLVSNGYLGDFSWSKAFPDLEHIDSLKYLAGITFIFVGIEMS
                     SVYMPRLKDATKNYTKGVFIALIGLVLLNVINAMLVANVVPDGKMELANITQPILIDC
                     QILGLPEVIGNIFSFMVFIGVLLQLSAWVTGPSKTIIQVAREGFLPPKFGFHKENKYG
                     VSRNVVLTQSIVISLFALLYGVMDDVSAVFLTLTNATTVIYCIVYILIAVSLLKMRKK
                     HPEFERPYRIGKNGNGLAWVVSCMLIFSIIVVVFATLGTATLSDALLVAAITVVMFVI
                     PLIINHFKKDSWGIEVEKSLEEK"
     gene            complement(62068..63945)
                     /gene="tdc"
                     /locus_tag="ACG842_000200"
     CDS             complement(62068..63945)
                     /gene="tdc"
                     /locus_tag="ACG842_000200"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004837 - tyrosine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HFQ0246771.1"
                     /translation="MSESLSKDLNLNALFIGDKAENGQIYKALLNELVDEHLGWRQNY
                     MPQDMPIITPEEKSSASFEHTVNKTKDVLSEISARMRTHSVPWHNAGRYWGHMNSETL
                     MPSLLAYNFAMLWNGNNVAYESSPATSQMEEEVGMEFAKLMSYKDGWGHIVADGSLAN
                     LEGLWYARNIKSLPLAMKEVTPELVAGKSDWELMNLSTEEIMNLLDSVPEKIDEIKAH
                     SARSGKHLEKLGKWLVPQTKHYSWLKAADIIGIGLDQVIPVPVDHNYRMDINELEKIV
                     RGLAAEKTPILGVVGVVGSTEEGAIDGIDKIVALRRVLEKDGIYFYLHVDAAYGGYGR
                     AIFLDEDNNFIPFEDLKDVHYKYNVFTENKDYILEEVHSAYKAIEEAESVTIDPHKMG
                     YVPYSAGGIVIKDIRMRDVISYFATYVFEKGADIPALLGAYILEGSKAGATAASVWAA
                     HHVLPLNVTGYGKLMGASIEGAHRFYNFLNDLSFKVGDKEIEVHPLTYPDFNMVDYVF
                     KEKGNDDLVAMNKLNHDVYDYSSYVKGSIYGNEFLTSHTDFAIPDYGNSPLQFVNQLG
                     FSDEEWNRAGKVTVLRASVMTPYMNKEEHFEEYAEKIKAALQEKLEKIYADQLLASEA
                     K"
     gene            complement(64236..65492)
                     /gene="tyrS"
                     /locus_tag="ACG842_000201"
     CDS             complement(64236..65492)
                     /gene="tyrS"
                     /locus_tag="ACG842_000201"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HFQ0246772.1"
                     /translation="MNIIDELTWRDAINQQTNEERLRELVEEKSISLYCGVDPTGDSM
                     HIGHLIPFMMMKRFQLAGHHPYILIGGGTGTIGDPSGRKTERVLQTMEQVQHNVDALS
                     NQMRKLFGKDANITFVNNYDWLSKISLLEFLRDYGKNFNINTMLAKDIVASRLEVGIS
                     FTEFTYQILQSIDFLHLHKTYDVQLQIGGADQWGNITAGLDLIRKLEGPEAEAFGLTI
                     PLMLKADGTKFGKTAGGAVWLDPKKTSPFEFYQFWLNQDDRDVVKYLKFFTFLSQEEI
                     EDLAKKVETEPEKREAQRRLAEEVTRFVHSEEDLKEAQKITQALFSGNIKELNAEEIA
                     QGFGKMPNVEISSTPENIVELLVSTKIEPSKRQAREDVSNGAISINGDRVTDLNFVIN
                     PSDEFDGKFVVIRKGKKNYFLAKVID"
     misc_binding    complement(65604..65809)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            66072..67373
                     /locus_tag="ACG842_000202"
     CDS             66072..67373
                     /locus_tag="ACG842_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HFQ0246773.1"
                     /translation="MEKKQKIILGATSALTIVILILSLFFVIRSSTKKEPIIPEKKIT
                     EKAKEPTDDKQQLVTQAKNLAQSYDYDQAIALLEKDSKNGSTETQQLLQTLKKEKEQL
                     IDWSDPTQISHVFFHSLIVDPQKAFHSQQAQGYKDYMVTVEEFNRSIEQLYQNDFVLV
                     NLNDLIQKDEQGNLSFNGLALPEGKKPLVLSQDDVSYYEYMDNSGFPSRLVVSNNNEI
                     KNVYKEKDKEEIGDFDMVPLIDAFVKKHPDFSYRGAKGTLALTGYNGVLGYRTSKSQY
                     GDNEKTKKEIKEAKKVAEQLKKDGWSFASHTWGHLNMTQASLEDVKKDNELWQQEVAP
                     ILGKTNILIYPFGADISDWQPYSAENQKFAYLKSQGFDIFCNVDASTPAWGQLGTDFY
                     RNARINIDGIRFQSDLDGKNPILDPFINVHDVYDKEARESA"
     gene            complement(67442..68041)
                     /locus_tag="ACG842_000203"
     CDS             complement(67442..68041)
                     /locus_tag="ACG842_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFQ0246774.1"
                     /translation="MNGFEKRAQEKKKEILQAAFDLMNTDIGTAGVTMDKVAENAQVS
                     KATIFKYFGSKEGLIYEVFFAFMNDLGQEAKALIAENLPFEETLIAMSENKIRYLNRV
                     NKQFYIDLMTYYTKKEKKEFSDAMDAYTQESFSIMLDLFHRGRKEGKVDLKYSDEFLI
                     LYFQAMVEGISKPEIYAKLLPYTAEWTEVLIKGIAPKKK"
BASE COUNT        20431 a        13815 c        12124 g        21759 t
ORIGIN      
        1 ttgtcacacc tcccttttcc gaataaactg tgttccagaa atagcttctt cgaaaataag
       61 ctgaaatttc acaaaaattt gagaagcaat tttcgtacat tccttcttct ttcttgaaac
      121 tgaccacttc tgtcacaccc tctcttctat cactttcctt ttttcacacg actcccggtt
      181 tctttgataa atctcgatac tttcaatttt cttgaatttt tcatttgagg tacacataga
      241 tacagacgat ttcttgctct agtcatcgca acataaaata gtcttcgttc ttcttctatc
      301 gtcgttactg gttctgcttt tatttcatta aagtcgatta ctttcttttc tgtcgctccg
      361 ttataaggca aacttccttc gttacaacca atcaaaaaga catttcggta actaagacct
      421 ttacttttgt gaatgctctg gatacatagt gcatcaagtt ttccttgtct gtaagcaatc
      481 aagcgggttt ttaattcttt agctgtcgct tgggtttctt gcgataaggc ttggagtctt
      541 ctgaaagtag catgctgctt tacttcttct cgtatccatt cttgatcact ctggtcgatt
      601 tccttagcta tacgtggcag atgaagatag ctttgaacaa gcgaatagat ttcttgatgc
      661 tggtctgttt tacgcaacgc ttcctcaaac tctcggattt ctgcagattt cgattttaaa
      721 ttggggctta tcgacaaaat ataatctgca acggctatat tttgatattt aggagaaaag
      781 tattgatcgc attcactttt tttcaggtag cagtctgcca tcagctgccg gagcaattgc
      841 tggtaggcct gatttttctt ttgcagattc agtgctgttt gccaatgaac aattttttga
      901 tagaaatgtt ggaaagactt gataccaaag ataccttcat atggactatc tgcttccagt
      961 acaaaaggaa tattcgtttt cagcagagat tggacaagct gctgcgtttg tgaagctgat
     1021 cggtataata tgattgattc cttatatttc ccattttttt gaataaatgt ctgaatttgt
     1081 ttgattagcc actgactttc tgctttagta tcagaaaaca aataggcttc cataacacct
     1141 gcttcttttt gtgctggaat catcgccttt ggtatccgat catttttatt atagctgatc
     1201 aatctatttg ctccttggac aatagctgcc cgagaacgat aattagttaa taagaaaaga
     1261 gttttggtag gatattcttt ttcaaagttc ttgatgtatg ctggctctga tcctctgaat
     1321 ccatagatac tctgatcatc atctccaaca acaatcaact tgatcctgtt ttcctttgtc
     1381 atccgtttca cgatttccca ttgaagtgga ttagtatctt gaaattcatc aatgaataga
     1441 taatcatatt tttggccgat ataatgctgg atctcctcat tctccaaagc gcttttcaga
     1501 ttttggagta catcattgaa ctctagcaat tgctggcttc ttttttgttc ttggagttcc
     1561 gtgagcaatc catacagtcg ttctgatatc ggctgtcgtg cattcttatc aatggaatca
     1621 tatttttctt taactaactg aaccatttct tctactgaaa ggttcatatt ctgccaatag
     1681 tctaaccgag aagcaatcgt tttttctcca tattgatcaa atatataaag attatattta
     1741 cgtatcagct gttgcaaaac agcattcact ttttgtgcag tatcctttga aaagtctcct
     1801 tgtattcctt gcattgctac ttcaggaaat tttcgacaaa tttcattgta tatcctaaaa
     1861 aagatactat ggaatgttcc gatatggaca gcggtttttc tctggctgaa attatctact
     1921 cgttttttta gttcatttgc agcgttcctt gtaaaagtga tcattagtat gcgttcttcg
     1981 tctacttgtt tttgcgtcat cagataaaca gcttttgcac agattgtttg cgttttgccg
     2041 ctcccagctc ctgctatgat tcgaagatgt ttcttcatat catatgtgat agctgctttt
     2101 tgttcttgat tgaagcgatt ataggaacgt ttctccatca ttttaaagaa agagctgttt
     2161 ccttcacttt cttttacttg tacttcttcc tcaataagtc cgggtgcttc gccataagcc
     2221 gcttcatagg tagcaatgat ttcttcttgt agccgaaaga cttgttgaag ctttttatac
     2281 ttgttctccc agcgtttcaa ttctttttgt aatcggagaa tttctttttc attcaactga
     2341 ttgatttcct tcccatcttt cttctttgaa tttgtttatt tcaagttggt tatttaaagt
     2401 ttcgttgtct attttttctt tatactaaca gacctttcta aaaatgaaaa tgggaagcga
     2461 tatgatttta gttttctgaa aatttttcct ttttttcaat cgtttatgct aagataagga
     2521 agacaaaaat attcataaaa ggtgatgaaa aaaatgcaga cttcctttca cgaggggcgt
     2581 acaataggac ttttgctgac atttatcgga ggagccatgg acagttatac ttatatccaa
     2641 tatggtgctt ttgcctctgc tcaaactggc aatatcattc tatcgatcat ccaagcattt
     2701 gacggagaat ggctaagtgt tggtaaaaaa gtattgtcta cccttttctt tttcttaggg
     2761 attcttctaa caaagtatct aattgattat tttagaaaaa aagaaaaaca tttctggcgc
     2821 ttgtttgttc tatattatga agcagtgatt tttttctttg ttagtttgac acccgtccat
     2881 gagcatcccg cattagtaac gattttgatt gcttttactg catcgatcca gtgggtagct
     2941 tttgataaaa tcaacgggcg tgcctacact aatttattta caactggaaa tttaaaaggg
     3001 gtagcaacga acttatatga ttattgtatt tctaaagaaa aagcagattt tgatcgattt
     3061 ttccactatc taagtgtcgt tcttgccttt atcgcaggag ctattgtcct tgttttctgt
     3121 catcatctgt ttggcgccca ttcgattttg atcgtttcca ttttattttt gatcatggcc
     3181 ctcactcaga catttcattt atggcaattt tatcatacga atcggttcac tatttgatct
     3241 caatgaatca aaaaaactaa gcaagatgct gctcacagag aaatatctgc gagtgagcaa
     3301 tttgcttagt ttttatatta tttagttgta aagattccct ttgctttcgg gttatttgta
     3361 gtgtagcgga tcgactgggc atctgcctct tctgtcattt tctttgcccg catcttgatt
     3421 tcttgtacga tttgttctcc acatgccgga tcggctacga aatcatattt gtccccatcg
     3481 atcactattt tgggacatat atcaaactgt tcataccact cttcgtatcg tcgattgagt
     3541 tctttatagt aggaatagag ttcaggatct tgttctagct gttcatattc ccggcctctt
     3601 tttttgattc tcgctaacat agtttcaaag gatacacgaa tataaaccag tagatctggt
     3661 ttcttttgtg gcgaaatttc ttctaactcc ttcagcatcg tttctaaaag accttcatac
     3721 tgctttactt ctacttcaga aacacgccct aaatctgcat tcaaatgaaa cagcatacta
     3781 tcttcgtaga tggagcggtc tagtacgttg tcatcatggc tgaaagcatc tttgattgcc
     3841 aaaaagcgtt tgttcaaaaa gaaaatctgt aataaaaaag tatacttttc tggattagaa
     3901 taaaatagcg gcaatacttc attatcttcc acgttctcat aaaaaggttt tgtttctaaa
     3961 tcttgggcaa gcatttcagt tagtgtgctt ttccctgcac cgatcgttcc tgcaacaatg
     4021 atcacaatta tcggcatcct tcctgtaata aaatctgttc ttcattttac tacttcattt
     4081 atagaaataa aagccttgac tttatctgct ccttaaaagc tacttctcta aaagtagcca
     4141 aaagtaactc ctattcatga aaataaaggc tgtgacaaaa gtcttgtcgc agcccttttt
     4201 tcaaataaac agtattctcc tgccaacacc tcggtcacaa ggcaatattc ataaaacatg
     4261 aaaagctgtt ttttaacatt attccttgta actcagagct aaacgggaga ctcacaacct
     4321 ctttatgctc tcaacatttt atctgttttt attcttctat tgtacttgat ttgcgtacaa
     4381 ctaactgcca gtgcagatta tgagcggtat cattgtcttc tactgcgcat ttcatgacac
     4441 gtatcatcac actattttca tagatttttt ctatttttcc ttttattggt gattcaaata
     4501 aatcagattt aaatgcatat gtttcgccga cttcaaacaa ttgttcattt tccataagaa
     4561 tccctccatc accttttata aggtcagtaa ttttttaaca taaatcactc gaaaaagcac
     4621 aaacttgtca taaaaagtac ttttcgtatc aaaactgtca ctttttttgg taaggttctt
     4681 ggaaaacgaa gattttttta ggagatcatg acggttttga tgtcaaaacc actttttatg
     4741 aaacaaaaga cgttttttat tttttttagc ataaaatttg tggtggaggt acgtatgaga
     4801 tgaaagataa agttgtgaac aaattattag aagccttatt cagcggagac aaatcggaag
     4861 aagatatggt gtatgtccaa gtaaaatttg tcttagaagt tttattgaat aatcttggaa
     4921 aactagcggt cgttctagtc ttttctctgg tgacaggctc atgggcggaa acaggaatca
     4981 cctttctttc ttatatctgc atccgcagat atgcttatgg attgcattcc gactctgaat
     5041 tcgtttgtct gctgtggaca ctactttatt tatggggcgt tcccttggtg atgaaacatc
     5101 tgcaattgac tatctccttc ccaatgatgg tctttttgtt aatcagctgc tttttgttac
     5161 tgcttcgcta tggatcaaga ggtacagcaa tcaatcccat tgaacccgag aaacgtcccc
     5221 cattattaaa gaaggcgatc agcatgtttc taattttttc tctcattacg ttatttttct
     5281 cagcttctta cttttctacc tatttgctac ttgggattgt tttagaaata gctacgttgc
     5341 ttcctattac aaactacttg atgaattttg gaggaatctt tcatgaaaaa aacaatcatt
     5401 aaacaattaa ctaacttatc tgactttttt gtatctggct gctgctttgg tatgagttct
     5461 gaattcaaaa tccctgaaaa attaaaaaaa taatacgcca attgtgctat aatttggtaa
     5521 aaatcaatgg gagctgtact ttatgttttt atggtccata gcgttgcaat atttgattta
     5581 cacaatttgt atcttattga ttgaccatca catccgatac aaatatctgt ttgcgggagt
     5641 cattctgatt tcttgtattt tgattggatg ggtctattcg attttaggga tttttactgc
     5701 tctaattgct ataggaatgc tgatgcttac ttttttcttc ttttcgactt cgcgtcaaca
     5761 attggtttac gcgcttcctt ttgggttatt gacgtcgctt ttaggagatc acttaacaag
     5821 catcggcgat ttttttgttt tgaaatcttc ggttattgaa ccaggagatt cattgcagta
     5881 ttttcacatt gtcttatccg cttttttatc tgctttatta gctttttgtt taaaaaaatc
     5941 gttagagact tggtttcaca ctgtggaccg ccgtatgatc ggtacgatcg ggacattgat
     6001 attatttaca tactatatca ttatttttta tacccgtttt tctggtgaaa cacctgatgt
     6061 cttagctttg aatacgagtt ttttcattct atatctgtgt gttggcctga tcatcttggt
     6121 ttattattgg aaaatctcta ataagaaatt agctgatcag ttattagagc aacaagtcag
     6181 gatgcaacag gattatatca aagatctaga aaaaaactat caagacttga gagaattcaa
     6241 acatgattac caaaatctgc ttttttctct tcacagctat atttcagaag gcgacttaga
     6301 aggattacgg tcttattatg atcaaacgat attacctaca cgccagatga tgaacctttt
     6361 tccggccaat ctaagtatgt tggataatat gaaagttcct gagatccgta gtttactgtc
     6421 attaaaactc atgatggcac aggaaaaagg gttgatcgtc cagcttcatt ttcctgagcc
     6481 aatcgaactg gatacgaaac atacggtaaa ccttgtacgt atgcttggta ttatcttgga
     6541 caatgcgatt gaaggagcta gtgctgcaaa gaagaaaaaa atcgaattga ccatactcaa
     6601 gaagaatcat tccgtgatga ttcgtgtgat caatacaaca acaaatacat taccgttaaa
     6661 ccagttgaaa caaaaagggt tctcaacaaa aacaaatcca aaaaacgaag gccttggctt
     6721 gtttatcttg gatgaattga caaagaccaa tccttatctt ttccttgaaa catcaattga
     6781 aaaccagcgt ttttctcaaa cgatttatat acaaataaaa taaaggagta atgatatgat
     6841 tcccatttat atttgtgaag acgatccaaa acaattagaa gttttatcaa cggtgatcaa
     6901 aaatagaatc atgattgaaa acttagacag ttatgtccat atttctacct ctgatccgga
     6961 agaacttctc aatgcagcca agatcagaac ttcttctttt ggtatttact ttttagatat
     7021 cgaactagaa gacagtgaaa tggatggtgt agcgatcggt aaattgatcc gtgaattaga
     7081 tcctttagga aaaataatct atgtgacttc ccatacggaa atgtcgatga aaatcttaaa
     7141 aagcaacatc gaaccgactg attatatcgt caaagaagat ctttatgatt taaaagaaaa
     7201 tgtcgagcat atcctgagca aaattttcga agattttcaa atgtcccaaa tccaaaaaga
     7261 tatcttcaaa atagagttta atgatgagat caaattttta ccgatcaaag acatccaata
     7321 tttttctact tcgctaggag cgccacataa actggaagtc catttgactc atgcccaatt
     7381 acaattctat gagaagatca aagaaatcga gaaaatgcat cggaatttta tccgttgtca
     7441 taaatcctat gttatcaata cacaaaatgt gcggtcgatt aataaaaaaa ccagagaagt
     7501 cacgttggca aacggcgaga ccatcccagc ctctatccgt ggattaaaga aattgatttc
     7561 ataaaaagaa ccgaatagtt tggaccgact aaatgaccag ccgatccaaa cccttcggtt
     7621 cttttttgcc tatcagtgca aaagcatttc ctgtgcaatt tcacgtccat ccattgtatc
     7681 tggataaaat gttggccaat tctccatttc tttcagcaat acttcttgac tttcatttcc
     7741 tgcatataaa tgaaaatgtt cggagtcagc cggtgcaatc gtgtgatcac tgaattgaat
     7801 atatctaaaa gcgccacttt ccttatcttc tgcttcgaat agatacctta cgccacggtt
     7861 tcctgcctcg taggtcaaga tttctttccc tacataacga taatttgaac gtttcgttgt
     7921 tccattctta aacgtaaatg ccatctcttt ctcactgatc ttgattgaag caatatctgt
     7981 ttgatagccc ttcgtataat attctttata ttcttgtgct gtcttatcct gattcaattt
     8041 ttctttatat tcaaaaacag gatcaaaagt cccatcttct acatacgtat ctactgtttg
     8101 ccaagttcct gcccaatctg aaagagagcg atcttgtaca tcctcgtctt caaaataacc
     8161 gttgtagacg ctcttttctt ttttgggggt caaagtatct tctagcgggt tttctgtctc
     8221 tgtcgttttt ttcaacgctt cgaggtttgc ttccatgata gaaagatagt tttctccatt
     8281 ttctatttgt tcattcgtca atccttccaa aggattcaat acttctaaag atacacctgc
     8341 ttcgtttgct aacgttctgg caatactatc tttggcgttt tcttcaaaat aaatatagtt
     8401 gattccatgt tctttaacaa agtgtttcag ttcagcaatt ctcgccgcag acggttcttc
     8461 actagaagaa agaccagcaa tcgatacttg attcaatcca tattctaacg ctagatagtt
     8521 gaatgcagca tgttgcgtga caaaagatct ttgttttgct tgcttaaagg tttcttgata
     8581 aagctgatgg agtgcttgaa gtttctcggt ataggctgct gcattttttg tccatacttc
     8641 ctgtttttct gggtaagctt caatcagctg atcacgtatg tttgctacct gcttgattgc
     8701 taaacttgga gctagccata catgtgggtc tagctcatgt gtatggcctt cttccccgtg
     8761 ttcatggtca tgatcatgtc cttcttcact tcccggaagt aaaagcatac cttctgtcgc
     8821 ttttatcgta tggacattgc cttcttgtaa tgttttttgg attgcgggga cccatgtttc
     8881 catgttttca tcattataaa caaagatatc tgtatcttgg atctttgcca tgtctttagc
     8941 agaaggttca taatcgtggg aatcagtccc tgctggaatc aataactcta cttctccttc
     9001 atccccgacg attgcttttg taaaatcata catgggataa aaggtcgtca tcacgctcaa
     9061 tttttcttta ctgtcctcgg ctttcccact tgccccgcaa gcagttaaca tacctgctaa
     9121 aaccagcatg gccaatatac ctatcaatcg tttcatttat gacactcctc ttctcatctt
     9181 ctttcatttg aatgttccga ttcgcttttc gcaaaccgta atagttacgt tttagcaaca
     9241 ggatttaatt tatcatatgg ttaaaagaac tgtcaagcgt tttcttattt taattgatga
     9301 gaaagtttat atgaatgatt gacgatattt ttgcaatcaa cctctatcat tttcctttta
     9361 tacccgcttt tgtctataat gaatttatag ataaaggagg aaaaagagtg aaaaaagaaa
     9421 cagatacaac gaatcgtttg atccgctttt taggaggaaa aacctcttac tatgttttag
     9481 gaatagttat cttacttgcg ctgatcattt attttttcca tcagatctcg tttatattca
     9541 agcctgtcgg aatcattatc gctactatct tgcccccatt attgtttgca ctgatcttgt
     9601 attatctttt ggaaccagtc atcaaacgtt tgagcaagca cctttcgagg aacttgtcag
     9661 tcattatcgt ttatgtgatt attttatttt tattggggct tggaagcata tggcttattc
     9721 cttttcttga agaacaggca aagacattga tcagtagttt gccagatttg tttcaagatt
     9781 ttcaagagag tctccgacaa tttttggaga aaacaccttt tgccgattca tttaatcaat
     9841 ttttcgcttc tattgacgac gtgacgtctg atatagctgg atttattgga aattattggg
     9901 aaagtggcgc acaacggatc agcaacattt tttcaactgt tacagctatt tttattactt
     9961 tatttactgg tccaattgtc gcttttttcc ttttgagaga tcctcaaaaa ttttatcagt
    10021 cagttttagc gattgtccca ccagcatttc gtacagattt taaaaatttg acaaagattg
    10081 ctaatcaaca attgggttca tttctaaagg ggcaaatcat tgcttcattt atccttggag
    10141 cagtctactg ggtttgtttc ttactgatcg gattagaatt tgctagtgtt ttagctattt
    10201 ctgctgggtt gttatgtatc attccatata ttgggccgtt tatcgccttc tttccaggtt
    10261 tgatcattgc attccaagac tctactttta tggctgtcaa atttgtcatt gtctggtttg
    10321 ccgtacaact tctccacggt gacttggtga ttcctcgagt aatgggtgat cgtttacaga
    10381 tccatccgat tacgatctta atcgttttac ttgtcatggg tgatctaatg ggaattgtgg
    10441 gcgttatttt tggtattcct atctatacgt tagtcaaatt actcgttact tttattttcc
    10501 gtaaattcaa acaacgctac aataaatttt atggcgataa aggagagtat gaaaactctt
    10561 cattttctga agaagattat tttaaataaa acacaaaacg tcgatcttac gagaatagct
    10621 cgtaggaacg acgttttgtg tttttgtttt aatttttcag caaagcaatt gcctggtatg
    10681 gttttagctc cagactttga tgaagaacag tatcggaata attatcgata agaatattgg
    10741 cttctgtgaa tccttctggg atggcgatcc ttgtatcttt atcatagaaa tttgttaata
    10801 caagcagctg ctgtccttca aattggcgaa gaaagccata aacttccgga tgatttgctt
    10861 cataagcatg ataatctcct tgtgcaacga ttggatatgt ttttctcagc cgaatcaatt
    10921 tcttgtaata ggacaaaata gaatcttcag aagatagttc tttttctaca ttaatctctt
    10981 ctagcttgcc tccaactttt aaccaaggtt tccctgtagt aaatcctgct tgttctgtgt
    11041 ctgaccattg catgggtgtt cgagagttat cgcgagattt tgctttgatc cgacgaaacg
    11101 cttctttttc tgtgaatcct tgttcaagga gtaacttata agcattcaaa ctttctacgt
    11161 caatatagtc ggaaatcgat gaatagtctg ggtcgatcat gccgatttct tctcccatat
    11221 aaatataggg cgtcccacgg ttcaaatgga tcatggttgc tagcatctct gctccttgtt
    11281 ttcgatactg atcgatagct acaaatcgat tgagcgcacg tggctgatca tgattattaa
    11341 aaaatagagc attccagccg ttaccctcac tcatcttttc tccccatgta tgaaatagtc
    11401 gctttaattc tgcaaaatca aatggaggag tagaccattt attcccattt tcataatcga
    11461 ctttcaaatg atggaaatta aagaccattg ataattcttc tctactaggt tctgtataca
    11521 agatacaatt ttcgatcgtc gtagagctca tttcaccgac tgtgatgata gtggaatccg
    11581 tcccaaacgt tgcttggttc aattctttta gatacgtatg ggtgattggt ttgtccgtat
    11641 attcaggttt gccatcattt tctttattat ttttcaattg ttcgtcttta ccgataacat
    11701 tgataacatc gaaacggaat ccttttaccc ctttttccat ccaaaaacga acaactttga
    11761 atagttcctc tcttactgct tggttccgcc agttcaagtc agcttgtgtc cgatcatata
    11821 agtgcaaata atatttcccc gtatctccaa atggcgacca agcacttcca ccaaattttg
    11881 atacccaatc cgttggctct tctcttaaaa tgaagaagtc ctgataatgc tggtcccctg
    11941 ctagtgcttt tttgaaccat tcatgttctg tcgaaacgtg attcagcacc atatcgagca
    12001 tgatttctat ccggtatttc tttgcttcgg ctattagctg ttcgaattcc tccatcgtac
    12061 caaagatagg atcgatggct gtgtaatcag agatatcata accgttgtct ttttgagggc
    12121 ttggataaat agggtttagc caaatcatgt cgatgcctag atcattcaga tacgggagtt
    12181 tttctttgat tccttttaaa tctccaattc catcaccatt actgtctttg taagatttgg
    12241 gatagatttg gtaaatgact ttgtctttaa atgtcatgga tatttacctc cattcaactg
    12301 atttattacc taccacaact tatatataca agttatcatt tattattata actgaaccga
    12361 aagcgtttgt aaaatttatc tttatacatt ctaagaggtc tgttgcccaa taaaaaacaa
    12421 gagatcagac atgtatttgt ctatcctctt gtttaaaata gcaaaatatt cttgtttctt
    12481 ctataaatgg tgttcaaaag cttttatcta cggcaatcag tcttctcaaa tgtaaaacat
    12541 gaggagctaa ttttatttga gtattctttt tgctcgatgc aggacggctt ttttacaacc
    12601 tctgattcat ggtgttcaat cagctgtcct tcggtattag ctcaaaaaac ggcaaaatat
    12661 gaaaaactat tttgccgttt gtttctctaa tactcaggtc aaaccgctga tttcacaacc
    12721 tctgttttta ttcattcgat ttcaacgcat caatcatatc gatatgcttt aatttgaaat
    12781 gggtaacaat catcacaata accgtaaata cgatagttaa tcctgctgat aagacataag
    12841 cccaccatgt gatcactaat ggaaagatga cattctccat agacgcttgt tgcaagatga
    12901 agtcagtcaa tatatatcct acaccaaatc ctccgatgat tcctaaaaga gtaaagatga
    12961 tattttctcg aacaatgtac atcgtgactt ctttatcaaa aaatccaagg acttttattg
    13021 tagataattc gcgtacacgc tcggaaatat tgatattagt tagattatac aagacgataa
    13081 aagccagcag tcctgataat acgacaaaga ttaagacaac cgagtctaag ttcgccattg
    13141 attcttcttg cgtttcgatt tgtttggaca tgaaagacgt atttgtcaca gcgtctgtgg
    13201 caagcagttt ctcggaaagc atttcttctt cttttgttgt catcttatct gtacgcagca
    13261 aaaatgcatt atccatcatc gattgatcag taactgaagc ataatattca ggggacatat
    13321 aaataaagct acctagatag tttttcacga tttcttttac ttcgactttc cattcttttt
    13381 gatcttcatc ataaagcgaa atatgatctc ctgtagataa gtcaaatagt tctgctactt
    13441 tttgcgtaat agcgattcct gttttaggca aggagcgtgg gttctcttca agatcagtta
    13501 aatggataaa acgatccaaa tcagctgttt tacttggtac gatcactgtc agactttgcg
    13561 aagcatgtcc tttttttctc aattctacgg tttgagtatg aatagctagg atctcattta
    13621 ctttttcttg cttctgcagc atatccgctg ctttttgacg actatcttcc gtggatgaat
    13681 ccaatgtaac gattgcttga tagtcaatga tcggtccaaa ttgtttggct gaaacagaac
    13741 ttagagaatc tttcaatccc acgcctgcaa ccatcagccc ggcacatcca gcaattccga
    13801 taatcgccat aagcattctt gacttatatc ggaagagatt tcggtaactg attttttgat
    13861 taaagctaag gcgtgaccac agcggagtga tgtattcaag aaaaatgcgt ttccctggct
    13921 tcggtgcttt cggttgaagc aatgcagcag gccgttctct taaatctttg aataaaacga
    13981 tcattgcagc gccaaatgcg gcaatgaaga atgcggcagc tgcttgtaaa atcggcggcc
    14041 agtcataaaa aataagcgca ctcccaatat cataccgctc gttagacagc tcaaaaatga
    14101 tccttggcaa aagattcgtt cctaatatcg ttcctaaaac aatacctatc cctgaggcta
    14161 gacttgcata gattgcatat ttttttgcga tttcaacttt cgtataacct aacgccttta
    14221 atgtcccgat ttccctgcgg ttttcttcta ccatacgtgt cattgtcgta aaggtgatca
    14281 atgcggcaat aaagaaaaag aaaacaggaa aaacattggc aatcgcagcg atacgatcag
    14341 acagatcacc aaattcttga aaaccgacat tttctgaacg ttcattgtat aggtatgatg
    14401 gtttagttaa attttcaatt ttttctgcat ttcgattcaa ttcttcctct gcagaggcca
    14461 gttcacgttc tttctcaact aattgccttt cttgttcagc aagttgagct tcttgttgag
    14521 ataatgtttg aacagcgatt gttcgttgcg cttcaggaag ctgctggata gttgctgact
    14581 gttgcgctaa ctgtttttta gcatcttcta accgtgttct tgcttgcgtt gtttgtgctt
    14641 tgccatccgc tattttttgc tgtgccggtt ctaattcttc gttggccgat tttttcagct
    14701 cttctaagcg ttcttcgggt cgatcttgta aatacttttc tgccttttct tgattttgct
    14761 ccattttatc tttgtaagct ttgctgtacg tattcaaatt ctttacgttt gtaaaatcaa
    14821 gatataagac agaataaata tctgttttaa aaacggattc aggtaaatag acaaaataat
    14881 cgacgctacc atttccgacg ttcgcgtatc ctctctccaa attgttgatg aacaatggag
    14941 aacggacaaa gcccgtgatg gtatactctt tttctttcaa ttcatctgaa tcaatctgat
    15001 aaatatcacc gattttatac ccttcatctt ccgctttttc atccaataca agctggtttg
    15061 gtttttccgg gagttttcca tcagttaatt gtagcttgtt ctgcttgtta tttttgttat
    15121 aagaaaatat ttgaatcact tcgtttttgc cagtattgga ataatagatc tgttttcctg
    15181 tttctacttt tgcatctggg atcttttctg cttgagcaat atctttttgt gtcaagccac
    15241 cagtagaaac gatttgcaaa tcagaaagtc ctgctgtacc aacatagtga tccatcgttt
    15301 tttgcatgac aggtccagca gttttgacac ctacaaataa aagtgttcct agcatgacaa
    15361 taaggatgat tgcaatgaat cgtcctttag attgccgaat ttcgcgaaaa cctgctttaa
    15421 gatatgttct tgttttcata aaatccccct accatacaat ctcatcaatg gatgaaggat
    15481 gatcatttaa ttcaacagac cttactttag catcattaat gtgaatgact ctatcagcga
    15541 tgggcgctaa agcagaatta tgtgtaatga tcaaaacggt attgccgtgt tgtctgctag
    15601 cgttttgcaa taatttcaat acttgcttgc ccgtttcaaa atccagcgcg cctgtcggtt
    15661 catcacataa aagaagtttg ggatttttag ctagtgcacg agcaattgaa accctctgct
    15721 gttcgcctcc tgataattgg gacggaaagt tattcaatcg atgagctagg cccacttgcc
    15781 tcaatacctc tacaggatca agcgcatcag gagaaacttc cgttgccaat tccacatttt
    15841 cttttgctgt tagattagga acgagattgt agaattggaa aacgaaccct acatctgttc
    15901 gtcggtaagc agtaagctgt ttatctgaaa attgcgcaat atctgtgtca tcaataatga
    15961 tctgaccttc atcaggtgta tccatcccac ctaaaatatt aagaatcgtt gactttcctg
    16021 ctccacttgg accaagtata atcactaatt cgcctttttc aattgaaaaa gaaatatcgt
    16081 catttgctgt aatggtcgtt tctcccattt tgtatcgttt gtattcattt tttacttcga
    16141 catacttcac tctgattcct ccattttcac gatatttctc gctatatcta tgcatcgaat
    16201 gcgttttctc acacttattt attttaccac tgaatttctc tagactaaag aaaggaatcc
    16261 tttaatttag ttgacaaata aacgatttat gatttactta cataaaaaac attgttattt
    16321 gaaagaagga acatcatgaa tgatttaaag gaaaatatga tttttgattt atcaaaaact
    16381 gcttttgtcg cgatcgacat gcaaaaagag atcgtaaata acggtacact ctctccacac
    16441 actgcagcat ctattctgac aaacaatgat ttgttagtaa gaacattgaa acgtaccgct
    16501 gccttgaaag ttttagtgaa cgtagacatc tccactttcc cttatttgag tcaacaaact
    16561 gatatgggtg gaatgactgc ttctgtacct ccagaattca ctgatttact tttgaaggat
    16621 tctttgaaag atacagataa tatgttgact atcacaaaat acaatccaag tgcttttttt
    16681 ggtacttctc ttgatcttca acttcgccgg cgtggaatcg aaactatcat tttaagcggt
    16741 gtagcgacaa caaacggtgt atatgctact gcattagatg cctttcaaca cgggtaccat
    16801 atcgtgcttg ctgaagatgc ttgcagtgat cgtgacaaag aatcccatca attgttcata
    16861 aaaaagatat ttccgaaaac tgcacgagta cgctcaacga aacaaataat cgaagctatc
    16921 caacaaagca aataagtact ggcacacgtg attttcattt acttcttttt gatttcatgg
    16981 gaaaataacc acaggattta gataggagta tgaacatggt gaaaaaaaag atcacaatca
    17041 aagaagtcgc taaactttcc ggcgtttcaa tcacaactgt ctccttgata ttaaatggaa
    17101 atacggagaa attcagtccg aaaactgttc agaaagtttt agctgccaaa gaagaattag
    17161 gctatcagcc tgactatttt gctagacaaa tgattaccaa ggaaacgaaa acaattggtg
    17221 tactcgttcc tgatatcacg aatccattct tcagtacact gatgcgagga attgaggata
    17281 tcttgtataa gcagaatttc gtcacgatat tatgtaatgc tgattctgat catcaaaaag
    17341 aaatcgaata tctagccgag ctcacacgtc gaggtgtgga tggattcatt atcgcgactt
    17401 ctgctgtctc tacagatgca atcaacgaaa atttgaaaaa acaaggccgg cccttcattg
    17461 ttttggatca gaaaaaatct gaaggattta gtgatgccgt acgaacagac gattttcgcg
    17521 gtggttacct agcgggcatg cacttgctta gtctaggaca tcaaacgatc gcccttgtct
    17581 atcctgaaaa tccaccggaa aatgtccacg ctcggattga ggggtttaaa tctgctcttg
    17641 atgtttacca aatccctcac gaccaactga tcctcttacc tacacagttc tcaaaacaag
    17701 gtggctacca gatcacagca gaacttcttg attctgctgc aactggtgtg tttgctttaa
    17761 atgatgaatt agcttttgga ttatatcgcg gattagagga agctggaaaa agcatccctg
    17821 aagactacag cattattggc tatgataata tagatatgtg cgagtatatc aagccgaaac
    17881 tgacaacgat cgcacaacca atcttcgaat tgggacaaac atctgccaag ttattgttag
    17941 atcggatcca attcccagaa aaagaatggg aagaaaaaag attacctgta cgattagaaa
    18001 aaagattttc aactgcaccc ttgaaatagc tttcaaggtg tgctatattt ttatcgaact
    18061 attaaaacgt tttactaaaa cgttttaaaa aataattttc ccatcaaaat aaatgagata
    18121 gttggaggtt tgtaaaatga acacggttac agtaattgga agtattaata tggacactac
    18181 ccttcgttta accaacatgc ccaagccagg cgaaacgatg catgcgcatg agatttttca
    18241 cgctggcgga ggtaaaggtg ccaaccaagc agtggcagcc aaacgttcag gcgcacggac
    18301 atcttttatt ggaggcgttg gggctgacag cgaaggacaa cagttattag atctactgac
    18361 aaaagaaaac atcgatacat ctgggatagc tgaaatccaa ggcgccacaa ccggacaagc
    18421 aatgattatg gtagacgcag caggagaaaa tagtatcttg atccatgcag gggcaaataa
    18481 tgctttccat gaacaagaag tcctaaaaaa caagcaactg attacaaaca gtgactttat
    18541 tattgctcag tttgaaagta gcttagatgc gacgatccta gctttttcta ttgcaaaaga
    18601 cgcaggaaaa acaacgatcc tgaatcctgc accagctcgt gaaacgattc ctactgaact
    18661 acttgaaaag acagatatca ttataccaaa tgaaacagaa acagaaatca ttacaggaat
    18721 cagagtgaca gatcataaca gtttggtcgc agcagccgaa aaactccatg aattaggtat
    18781 tggaacagta atcattacac taggcagtgc cggcgctttc tatcatacag aaaaagaaca
    18841 cggtatcgtc cctgctttta aagtggatgc cgttgacaca acagccgcag gtgatacatt
    18901 tattggcgca ttaagcagca cacttcagcc agaccttagt aacttgaaag aagcgatcct
    18961 ttatggaaat ctagcctctt ctgttgctgt gcagtcttat ggtgcgcaac cttctatccc
    19021 ttaccgcgaa gctttagaac aatcagcagt ctaaaattaa caatgagagg aagaaacaaa
    19081 catgaaaaaa ggaaaagtaa tcaattcaga tatttcacga gtaatcgcac aaatggggca
    19141 ctttgataaa ttaagtatcg gggatgcagg catgcctgtc ccctttggta ctgaaaaaat
    19201 agatttggct gtctcaaacg gtcttccaag ttttttatct gttttagaaa acgttttaga
    19261 agaacttgag gtacaatcga ttgaattggc ggaagaaata aagacaatga acccggaaat
    19321 attggaaaaa atcaaattat tgttaccaga tacaccgatt agctttatcc cacatgaaga
    19381 aatgaaaaaa gagttgaata actgtcatgc atttattcgt acaggcgaaa tgacacctta
    19441 cgccaatatt atattagtga gtggagtggt cttctaatgg aaacgacaat gaatcctgaa
    19501 cagatggtac tgtccgagca gattcatatg ctgattgaac agttgggagg aaaagacaat
    19561 attgattctg tccttccatg tgcgactcgg cttcgtatca aattgaaaaa acagccagta
    19621 gccaatgcag aaaaattaaa acagcaaatc ccttccatta agtatttcat tttactcgat
    19681 agatatgcag aaataatcat ggatcaggga ttggctcctg cttacaagga attgacgcgt
    19741 caaatcgatc attcagctcc gaaagaaaag ctaaatccga tcgaaaaact atttggtttg
    19801 atatcggaaa tctttacgcc gatcattccg ctgtttgccg gttctggtat attaaaaggt
    19861 ctggttgttt tagcgactac gattggctgg cttagtgaaa caagcggaac ttatcatatt
    19921 ttgtctgctg cgtcgaatgc cgtcttttat tttctgccaa tctttcttgc tttttctgct
    19981 gccaaaacgt ttaaagtgaa tggatatatt gcggctgtca ttgctgcagc actgattgaa
    20041 ccgaatctga cctctttact ttcagacagt ggcaaagcag ctactgattt tctggggatt
    20101 cctgtagtac tgatgcagta tacgtctact gtcatgccgg cgattttagg tattttcttt
    20161 tattcttttg ttgaaaaatt cctcaaaaaa tatattcgcg aaaatatgca attagtattt
    20221 gtacctttac tttcattagt tatcacggtt cctttgacgt taatggtatt cgggcctgcg
    20281 ggtgtttacc ttggcgaagg tttagctgcc gcaattactt ggatgatgga tattaacgga
    20341 ccgatcagtg gcgcaatcat cgctggtggt tggaatattt tggtaatatt tggtatccaa
    20401 tgggctgtaa accctgtcat gatcagtaat atctctgctt atgggttcga ccgaatcgtt
    20461 ccgttgacag gagcagccaa ttttggaatg gctggtgcag ctttaggtgt atttctgcga
    20521 agtaaacgtt caaaaacccg ttcgatctcc ggttctgctt ttgcttcgat tttacttgcg
    20581 ggagtgaccg aaccaactgt ttacggaatt gcgatcccat tgaaaaaacc atttgttgct
    20641 gcttgcattg gtgcagctgc tggtggagct gtaatgggat ttgcccaagt gaaagcgatc
    20701 gcttttgtat ttggtagtct gaccacattg cctgcattca tttccggtac ttttttctgg
    20761 tacttagcag gactggctgt tagtctagtc gtagcaatga tcactactct cgttagcggg
    20821 tttgatgaag acctaatgtc ttatgaatag ttaatgaaaa agactgaagt atgagatgtt
    20881 tatcacatat ttcagtcttt ttctttttag gatcattatt tttataaatt tttgtgatac
    20941 tttccaaact ttggtataat ttacatatgc ataaccattt ttatccccac ttgtttatcc
    21001 ccaaacaaag gtggtctatg caataaaatg aacgtcaaag aaaaacgaat gaatgctcgt
    21061 tgcttattta caaattgttt tatttagaat acacagacag atttatgaaa agaaaaagct
    21121 tttcgacacg attatctgtt ccaaaaaatg tctaatgaat gctgaataag acagaaaatc
    21181 aatgaaagga aaaggactag tttattatga aaaataaaaa aacgatacgt ttgctagctg
    21241 ctacttggct gattgctccg tctgttttgt cgaccaatac cgtatttgcc gtagataatc
    21301 aaatagctga tcaaacagtc acaacagata cgactacatc cagctcagct actagtgaaa
    21361 caacggattc ttttacagaa gacccaactg gaacaactaa ctcaacaaca ggatcgaccg
    21421 agccaactga aattgatccg gctgatccat cagaaaatga aaagccagcc gagacatatt
    21481 cccttacaat tgctccttct ttgattggta agatccaact gactgcttcg acagcggatt
    21541 cccctgaaca gcaacctttg gatattacgg aaagtggaac ggtagaaggt ttattagcgg
    21601 aaacgaaggt aacttacact attacgccag ctgaaggtga acaaattgct tcagtcgctg
    21661 ttaatggtgc caaagaagta aattatgatg caaagacctt tacagtaggt acgaacaatt
    21721 tatctattac tgcagaattt aaagctggac cggatacttc aacagagaaa ccaactgatc
    21781 ctagtgaaga agtccctagt acaccagatc cggaaacgcc aaacaaacca gatgtgggta
    21841 ataacaacaa taataatggt tcaaataatt caaacaataa taacaataat tcgaacagcg
    21901 ggaattcttc gaatcaaaca aacccatctg gatcgaataa caatggaaat actcagagag
    21961 aaccaaacca tccaaacaat ggttcatcga atggcggaac gaacaatgct tcatctcgtc
    22021 caaatacagc aaatcaaagc ggtatagaga atccatcttc tgcttcttct gattttgttg
    22081 taaaatcacc aattgatgct gtattgccta caaatacaac gaatgtccaa caagcaatcg
    22141 ttcgagaagc attcaagcat cttggaaaac catatgtatg gggagcaaaa ggtccaaata
    22201 cttttgactg ttctggtttg acttattacg tatatatgaa agcaaccggt cattatatcg
    22261 gtggctggac aggcgaacag caatatgcag gtacgcagat tccagtaagt caggcccaac
    22321 ctggcgacct cgtcttctgg ggaccttctt ccggtgttac tcaccatgta ggtatctata
    22381 ttgggaacgg acaatttatc catgcgcctc aaccaggaga taaagttcga gtaacttcta
    22441 tttcagattt cacgcctgat tttgctgtcc gagtaaactt agcaggttta cctgctgctt
    22501 caggttcgct gacaggcggc tcatctattt tagatggatt agatggctcg ttccatttca
    22561 gtcaaaacca aacaactgat cagttcttga aaaagattgc agatgatgca caggaaatcg
    22621 ggcaaaaaga aggaatctac gcatcagtca tgatggctca agctatcctt gaaagtggat
    22681 ctggaaacag cttattatca agcgaaccaa atcataacct ttttggtatc aaaggatcat
    22741 ataaaggaag cagcgtgacc ttcaatacac ttgaacaaga tagttccgga caaagctacc
    22801 aaatccgcgc acaattccgt aaatatcctt cttacaagga aagtttggaa gactatgccg
    22861 acttgatcaa aaatggactg actggcaatc ctgatttcta caaaccaact tggaagtcgg
    22921 aaacaaaaga ctacaaagaa gcaaccaaat atttggaagg acgttatgct acagatcgcc
    22981 aatattcaca aaaattgaac gcgatcatcg aagcgtacga cctgacaaaa tatgatgaac
    23041 cgaaaaaaga agcagcaaca actgaagaag caaacgctga aatcctcaat atgtctaagc
    23101 gattcgatgt gccaatcaaa tggacaacag atggcgtgcc aatcacagga ttagaatttt
    23161 caacgataaa acgcgtcaac aacgcttctt ccatcttccg tgtcgcttca atctgggatt
    23221 tgtggaacaa ttttacggca agacagatac cagaaacaac tactcgtaca gttaaagctg
    23281 caactgttcc tgtactaacc ttgttaagtt tagacaaatc attctggctt gatctgcctt
    23341 taaagaaata gaataaatta aaaaggggat gtgacactgt cacactccct ttttaattta
    23401 ttctatttct ttatatttat tttgtattcc gttacaaact cgcgtacttg gttaggttcc
    23461 agagtgacat ctccaaaact ttcaagtcgc tccgctcctg gcagttcttg agtctcaaat
    23521 gtgataccgc catgatttac taatttattc ccgcgcattt ccggtccatc ttctccaaaa
    23581 tttgctgtaa aaatcacgat tcctgaagca tctgttttta cttggatcga tactttttca
    23641 tctgggcttg tcaattccgc agcatttagt aaaccagatt gttttttcaa caaaaatgga
    23701 tgatcgatgc cgcccactat cattgattgg cgaaaatcac tggcaaagac ctcacctaat
    23761 tttttaggac gttggaagtc aaatgctgtg ccacttactg aaactacttc tcctgtaggc
    23821 gttgtatctt tattgagcgt agcaaataat cggctattca accaaagtat atgttcgtcg
    23881 attgactgtg ctggatcacc ggttaaattg aaatagacat gatttgtagg attgaatact
    23941 gttttgcggt cactgatccc ttggaccgtc actcgccatg tatcatcgtt cgtcagcgta
    24001 taccgtactt ctacatctaa agttccagga aatccgtttt ccatatctgg gctagtctga
    24061 tagaaaatga ccgatgcttc gttttcacca ttgatgactg tataatgcca ctgcttggct
    24121 tcaaagctag gtgcaccacc atgtaaacaa tgaccagtgt ccgggtctgt ttttacttcg
    24181 aacgtttcat catctaattt gaatgtgcca ttcgtaatcc gtccagccat acgtccgatt
    24241 gaagcaccga tataagggtc tttttctaga tattcttttg cttcatcaaa acctaagatc
    24301 aactctcgtt tttcattatc gattattgtt tgaaaatgaa caacccgtgc cccaagatca
    24361 ctgacagaca aagatgtccc attttgattc gtgatcgtaa tcaagtgata gttttcgcca
    24421 aaattttgtt cattgatttc catgttcctt gcaccctctt ttctttcatt tgcactgatt
    24481 ccagtataca tggaaaccgt tttcttaaca gcttattccg tgagtaacga gaaaaaattt
    24541 actatattct aataaaagac cgtgaaaaaa aggttgtgac aaaaatattg tcacaacctc
    24601 tttttccgaa caaacggtgt tcaaaagctt gcatctgcgg taataagccc aaccaaacga
    24661 aaaatatgag gagctatttt tgcttggctg ttcttattac ttgatgcagg acagcttttt
    24721 cacaatctct ttttcaatac gattccagta tataaccatg tcgtcagtac atagtagatg
    24781 aaatacaata cgaagaatat cgtaaacgga acccaaatat ctttgtatgg attttgcatg
    24841 aatagaccaa acatttgtaa tccgaataag acatggacta cacctaagat tcctggtgct
    24901 aagaataaca cgcctacttc tctacggatc gattgtttaa gcaacccgcg tcgtgttcct
    24961 atcttttcaa gcatttcata acgagcaatg tcactcttgc ttcctgacaa gatcttgaac
    25021 atcaaacagc tagcaagcat ggtcaagaaa gctaaaccta ggaagaatcc cataaattca
    25081 aaaccagaga atagaccatt atacacttca tacatttcat atttctggtt gctgtactct
    25141 actttttgca aagatggatt gttcatttta ttttgagcaa caagtgtttc gattggtttc
    25201 aggtttgctt ggaagtcttt tacctgaact agctgtaaag tggattgtgg caagttcagt
    25261 tgatcaaatt ctgattgact gactaccttg atttcttttt cttgctgttc cggcaattca
    25321 tagcttcgaa gttcctcttg tacagtcagt tcattcattt ttttagcagg aactttttct
    25381 ttcgtgatct tcggcggtgt tccatcttca tgttttatca tgatcaatgg ttgagccgca
    25441 aattcacttg cattatagta aatcgttccc gcatcttctt tttgagtata agaagcagaa
    25501 agtgttggtg aaagttcttt gattttttgc tgatcttctg cccgaggatt attcatcact
    25561 agatcatatc cagaattgtt ttctgtcaag tctgcaattt catttttaaa acctaatcct
    25621 accgtcaaag cgcccagtgc caaggcaaac agcatagcga ccattgagag catttgtgta
    25681 tattcacgaa tacgaaagtt taactgtgac aatgtgaaat tgttcaattt tttcaatgaa
    25741 atctgttctg atttctttag taatgataaa aagaaaatga tcacagaatg gaaaatgaaa
    25801 taagttccta atacgatcgt aactaaggct acccctatac caaagacttg ataggtcccc
    25861 acgtgtttca tcatatagta accgcagcct agaaaagcaa ttcccagtac tacttcaagg
    25921 aaaaacaaga atttatgctg cttgattctt gttggtgtct gatccgcacg aatcaatgct
    25981 aaaatcggtt ttttgacaat cgaaaaagca ttgacaacag cagctaataa aaacaaaaca
    26041 gcaaaaaaca aaacagtgat caacaacccg tgaatactaa aaggtgtaaa atgcgaaata
    26101 ttgagatcta actgatccac taataatcgg tggacgatac ttgtcaatcc tacaccgaca
    26161 gctgaaccca ctaaagtagc tgcgatccct actaagaatg tttcagaaaa aatcatctgt
    26221 gcgatttttc tgccttttgc tcccagcatc ataaacattg catagtcttt ttgccgcatc
    26281 gtcatcaaaa aggaatttgc atataaaata taaacgaatg tgatgatccc tagtaaaaca
    26341 gaaccgaatc ggaaaatgat aactgtagcc gcaacgatcg tattactttt caaaaaagct
    26401 tcgttgcttg ccattgattc aaacatgtaa aaaatagcag atgccataac taatcctgag
    26461 aataacacca agtagtctct taaacggctc ttgatcccag taagtgataa tttccaaagc
    26521 atagtcaatc tccccctact gttccaagtt tccaagaata gatagtattt ctcgatagaa
    26581 ggaatcacgg ctttgatctc cacgtttcac ttcttgatgg ataccgccat ccttgatgaa
    26641 taaaatacgt tggcaataac ttgcagaaaa cggatcatgc gtcacaagaa gaatcgatac
    26701 atgatctttt gtattcaatt cgtccatcgt atctaaaaga tcccgggcac ttttggaatc
    26761 cagcgcacct gtaggctcat cccctaatag aatcgttggt tgtgtgatca atgcacgtgc
    26821 ggcggcgacc cgttgttttt gtccaccgga aatctcagaa ggatagcttt ccaaaatatg
    26881 agagatggat aaacgttctg caattttttg gacacgttgc ttgatttctt ttggtctgac
    26941 gccttgtaaa gaaagaggaa cagcgatatt ttcttgtgct gtcaagcttt ctaataaatt
    27001 gaaatcttgg aaaataaagc caatctcatt cgcacgaaaa tctgctagtt gatttccttt
    27061 taatgtggtc acatcttttt gattgattcg gatatgccca tctgttggtt tatctaatgt
    27121 ggataaaata ttcaaaagtg tcgacttacc agaaccagaa gcgcccatga tcccaataaa
    27181 ttcgcctttt tctacatcga agctgatgcc gcttaatgct tgcgtctttt tctcattgtt
    27241 ttttccgtat acttttgtta cattctgtac ttcgactatt ttgttcattt ctttccctac
    27301 cctttaatta gtttttccag tgctgttttc ggcacgtctt ttttttcgac ttcttcatac
    27361 gaagtgacac ctttattttt gttccaggtg atttttaaat atgctccttt acgaagcggt
    27421 cgagtcaatt gtcccttgaa gtccagttct ttttccttac cctctttcgt atagccaggc
    27481 aggatatatc tatagttgac tgtttcttgt ccagagtctg attttgaaac tgtttttgta
    27541 ccatctgtcg taatttgtac atagtattct tctccaccca taactacgta atctgcgatt
    27601 ttcacccctg caagtaccac aacgatgaaa acaagtagac cgatcagaag ttttttcact
    27661 ttttcccctt ctctcttact ttatatactg attatagaaa aaagcgaaca attctgcctt
    27721 cgaatcaact atcattcttc tttcaatttt gtcattttct ttaaaacaac taaaaaaaga
    27781 gttcaagaca ataatccgtc ctaaactcct tcttcctatc ttacaaaaag tatgtcactt
    27841 acggtctttc atgtcgcctg tattgataac aggttgcgtt cctttgtctg taatgattga
    27901 gacaagtaaa ttattgatca attggacttt ccgatcatcg gtgaaattga tttcttgtcc
    27961 atcttcgatt tgctctaagg ccatttgagt gattgtgaca gctccttcaa caatggtttg
    28021 tcgagcagac aaaatggctt ttgcttgttg acgttggagc atggcactag cgatttctgt
    28081 tgcataggct aaatggttca agcgtgtttc gattacttcg acacctgcta ctgctaaacg
    28141 ctcttgcagc tctttttgca actcgtcaga cactgccgtc gtgtttcctc gaagcgtcaa
    28201 atcattctca ttgaatgtat catacgggta ttggctagcg atatgccgaa tggctgtctc
    28261 actttggatt tctacaaaat cttgataata cgctacatcg aataatgcct ttgctgtatc
    28321 aacgacttta aagactacta ctgctgagat ttcgatgggg tttccatcca aatcattgac
    28381 ttttaaaaca gaactattga aattccgaac tttcaacgaa acagtcattt tttgtgccaa
    28441 aggtatcgta aggaaaaagc cattttctcg aatcgtacct aaatattgtc caaaaaacaa
    28501 aattactttt gcttgattag gactgactac agttgccgaa cttaacaaca ataacgtgat
    28561 caaccaaaga aaaatactta aaaaaagtgc ccaaactttc gcttgccaca tacctaaata
    28621 gaagaaaaac aatccaacta aagctaatac gaccaatccg acaatccctg cataaccatt
    28681 gatataaaat gctctttttt ctctcatgct acttcctgcg ctcctttatc cttcaataaa
    28741 tatctaatgt tactttttta attttttttt cgaatacgcg ccattacacc ttctagttct
    28801 aactctttgt cttcagtgat gtaccaaccg cttgcctgtg tccctccggc tgcctgcggt
    28861 aaataatcca attttgttcg aacccagtgc ttttcttctg taaaatagtc ttctaccaag
    28921 acctctagat ccatccctgc tgaaagaggc aaatcttccc gaacagattt aaagataaat
    28981 cgttccttct tcccatcata cttcaatatt cctgtatcga taatcggtaa agaataataa
    29041 tccagctctt tcttcaccgt caataaatgt ttgaaaactt catggtacac ttgctctaat
    29101 tggcgacctt ctgaattgtt cgggtaatta accatcactt tttctttgtc tggatcatag
    29161 aaggtttggc ttagttgagt gatataagta tgtaatcttt ccatttctgt ttttaacttt
    29221 tctctttgac cttcttctac cttcatatac tctttcaccc tgttttcttt ttagatattt
    29281 tctttcttat tttactatag ttatgtgcgt taagcgatga aaaacagcaa agattggaag
    29341 gatctttgct gttttttctt aaaatatcca gttgagtaca ggatcaatct gtttcattgt
    29401 cctcttgatc cgtcgtaata ttgatcgatg tgtctttagc agtgatcttt agctttttct
    29461 gtccttgttc taacaaagta tttgttattg gctggtagtc tttttcaata taagattcac
    29521 cagacgtttg gatagtatag ccactgtttt ccatctcatg caaagataaa ttgccaccgt
    29581 ttaggatgac ttttccttct ttgctgatca aactgtcttc tagatggaag ttgctgtttt
    29641 cacctgtgac gatcaagtta tccacattgg cctcatctaa tcgaagtgag cctaattcat
    29701 ctttcgcagt tattgttcct cctgtaaatt cattgaacga tacccagcca tcatgtgtct
    29761 tagtattcac ttttttgatt gatacttcgt tgatatcaat tgcactatta ttcccttcga
    29821 ttgtcaattc agataattca tgatctgcag ggacaacaat agtgatttgt gcacttgtgt
    29881 cttctaaact aatgattgca cctgttgatt ttttgactgc attcacagtc aacacgccat
    29941 ttttctcctt tatttctggc ttttctgtct tatcataagt cgtttttatc tcaaaatcca
    30001 atccgcgtat gaccgaaact gtttgatttt ctgcatggat gacgattttg tttgcatcct
    30061 tgattttttt tgtctcagta accatatcga tcaaccgagg accattatcc caagtcagac
    30121 tcttttgagc gcctaatgta taagttgcac ctgctaggag gccaccagta agcatcagac
    30181 caataccggt aatcaaaaaa tgtttcaatc tcaatttatg tgtcctcctt caaacgcccg
    30241 ctgaatgcag atctcttttt gtaataaaac gtcgtcccat ccatttaaca aagttcatca
    30301 tgacataaaa aagccctttt gttcctttca gcaacagtgg cgaaataaat actgcgcttc
    30361 ctgtggcgaa cagaccgaca ccgataaaga ataatccact cagaatcgat tgtccaaaca
    30421 cactgattcc tgagatgatc atgaataggc caacacctaa gatactgata cataaaacat
    30481 agatactaaa aatgagcgct agtaaagcaa ccaatagtcc cccaataacc aatataaacc
    30541 ccagtgctag tggaatgagg acaggagaag caaataaacc gataatgatt aaccaaatca
    30601 ggcgaatttg tctttttgga tttttcacta actcgttttc tgcttgttct tcttctgaaa
    30661 agtaactgat ttttaatttt aaggcaaact gctttggtgt accatattca tcaatgatct
    30721 gatctaatgt ttttccagaa tctaaaaaat attcctcata atactggatc aaatcaaaac
    30781 gatcgtcttc aggaatttct tgaagatgat ccacaacttc attcaaatat tcattcattt
    30841 gtgctctcct ccaataaccg gtctaaagaa tctcgaaaaa caatccattc atcaataatt
    30901 tcttctaaat gacgacttcc cattggtgtc aacgagtaat atcgacggtt tctgccttca
    30961 aaaggttcat cataggtctc aagatatcct tcttttttca atcttctcaa aaccggatat
    31021 agtgtagatt cactgacaga gagactttct tgtacgattt ttgttaaggc atacccatac
    31081 atatcttcct tgcttaatgc tgctagcaca gtaccatcaa gaagaacagt tggaatctgg
    31141 atgtgcatta cgtcaccttc tattctcaag tgtaatattt tatttcaatc tatattatac
    31201 aacatataat atagtttcaa aatcagtttg tcaaatacaa aaaaactgac taaaagattt
    31261 gatccttttt agtcagcaaa aaatggcttc tctttgattt ttattataac agtggcataa
    31321 tccaatttgt ccacatccag ctcagtggca tgataacgat cattgcaggt atcatgtcag
    31381 ctactgggaa cattttcacg ttgatcattc taaaaccggt agcgaccatc aagaatccgc
    31441 cgcaagcttt aaaatctaag atcatgtctg gtgtagtcaa tggataaata aattttgcca
    31501 ataagaacaa aatataaaaa ataatgaatt ggggaattgc tatcacagaa acgacatacc
    31561 ctaaattaca cgcaaaaata gctgctgtga aaaaatcaag gacagattta gaaatcaaaa
    31621 tcgtactgtc tcctgtcatc ccagaatcta aacttccata gatccccgta ccacttgcac
    31681 aaaatagaac aatgaccgtc actaatgtgc tgataaattc atcgtgggtc ataccaagct
    31741 tttcactagg gaagatttta gaaataggta cttgcatcag cgcagccccc ttatttatcc
    31801 cattacctaa atgaacaatc aaaccaatcg ctgttcctat cactaaagca aaaataacag
    31861 ccggcatatt tttcatcggc gcaatggaat agattcccat ccccatcgaa cacacaccaa
    31921 aaaccatcgt cagctgtgct ttaaaatgtt cacttaattt atttcctact aatccgccaa
    31981 ttagtccgcc aaacaaaacg gaacctacat tgatcaaaac tccagttggc atcaaaaaac
    32041 tcctctcttt ttctacgaag cgaaaaaaat cagaaaaatt tctgtaatta ttgctttcgc
    32101 tgcaagaaag attgtaacag acagagaaga ttacaccaat tcattttttg tgttaaagta
    32161 ttctttattg gaataagtag gaggaatatg atggatatcc ataaattcga agtttttctt
    32221 gatttagccg aaacaatgaa tttcaccaaa actgctgatc gacaattcac cacccaagga
    32281 aatatttcta aacagattct ttcgttagaa acagaattag acgtcaaatt attcgaacgc
    32341 gcccatagaa aaatcgaatt gaccgaagct ggaagcttat tgctcccata tgtaaaaaat
    32401 gtcgtagaac aataccatgc tatgcaagaa gtgcttgaaa cctatacgaa agacaacaat
    32461 ctttcattaa atatcttaac gataccgaca atgatcaatt ataaaggttt ttccaaaatc
    32521 accgagtttt tgaaacgcca tcctgaattt acagtccagt tgaaagaagt ggaaagtgtc
    32581 gaattaactt acaatgacgc caatgaacat agtgacacga tccactttgc tagaagtttc
    32641 caggccccac caagttcgat tgagtggttg cctactgaaa cagacgattt tgtagcagtt
    32701 ctacctaaaa atcatccttt agctagtaaa aagaaattgg atctaagcga actgaaaaag
    32761 gagaactttt tgctccttgg ccccactacg aacttgtatc aacctgtact agatctgtgt
    32821 cgcgaagctg gatttgaacc aaaaatctct tataaaggag caagaatcga cttgatcatc
    32881 aatatgattg caaatgacat ggggattgct attgttatgg aaaaaacagt caagaatcta
    32941 ttaaaagaaa atactgtgat tctccctatt tctccaacaa cagagagtta tcttgcattt
    33001 actcgtaaag tcggtgagca ttctttggct tcagatacat tttgggagta tttaaatcaa
    33061 gaagaaaact aagccctaca ttttctgtcc atctatccac ccaaaaaaag agagcgacgc
    33121 atgactttcg ctctctttct tgcatcatgt ccttgtttca atgacacctc taacatttct
    33181 cgtagttcaa taagaaagca gcgattttta tgccaaaaga accgtaagaa catttcccta
    33241 atcacaaaaa aacgactaaa aacccctaaa aaccttcaat agaattttta ggggttttat
    33301 tctttttcct attgtttact taaccgtatc attgctgtcg ttcctactcc ttcgatactg
    33361 ctataatcaa tacgtccatc atgcagttct agaatttttt taaccagtga aagtcctagc
    33421 ccattcccag aaatttgacg acttgtatct ccttgataaa atttttcaaa cattcgatct
    33481 ctggtctctt cattcattcc tacacctgaa tcggtcactt ccagtacaat ctctgtggct
    33541 gtctcaaata gtttgatatg aatctgtcca ttgatttgat tgtactttat ggcgttatcc
    33601 atgatattca gccacacttg gtacaaaaac tcttcattgc ctgtgtaatt tactgcagca
    33661 agttcgatat ccaattctag ctgttctttc tcccatttag gctgaaaaaa aacgattact
    33721 tctctgattt gttcatctaa tcgatactct ttcttttcca gttggatacg ctggttttcc
    33781 agtttgttca attttaagat gttctctgtt aattgtgaaa gctgcgtgat actttcgata
    33841 atccgatata aaaatatttg gcgttcttcg tcagagagat ttggggcttg taacaattga
    33901 acatatcctt ggattgttgc tagtggtgtt ttgaattcat gggatacact ggacacaaag
    33961 tcattcctca gtgtctcgat actattgagt tcttggacca taacgttgaa atctttatac
    34021 agttgctgta cttctgctac tttctgctgt tcatccattc gaatcgaaaa atctcccgtt
    34081 gctaccagac tcatatttgt ccctagcgca ctgattggat gcaggatacg ccgcccaaca
    34141 aaaatcgata ttcctgtacc cacgaccata ctgaaccccg caaagacgaa taacgggtat
    34201 aacgttggtt ttccagccga ttcagcaggc gagatagaat gtcgagacaa taagaaaata
    34261 aaaccgagaa aacacagcag tgtcactaag atcgaaatga agacaatact agcaaaatag
    34321 atccataatt gtgaaaatag cttttttttc atctttaaat aaccgcctta tagcctagtc
    34381 cccgtactgt gatgatttga aaatcttccg tcttttcaaa acgggtcctt aaacgtttga
    34441 tatgtacatc gatcgttcgt tcttctgtat tcgtatctaa tccccaaata tcatccatca
    34501 actgttggcg agtgaagatc ttgttgggat aagacagtaa tttatacaga agcagaaatt
    34561 ctttttgagg cagaatcaac gaatgctgat cttgtaacac tgtataagca tctagctcta
    34621 attttgtttg tccgattatc aagctactgc tgtaattgat ctttgaacgt cgaagcaatg
    34681 cttccacacg taggatcatc tcattgacat cgatgggttt tgtcatataa tcatctgctc
    34741 ctaatttgaa cccgcgtttc ttatcctcaa aatcggcctt tgccgtaatc attagaacag
    34801 gtgtgtcaaa tctggtgcta cgtaatgagt ctagcaagtc atagccatcc attttcggca
    34861 tcatgatatc tgtaatgatt aattctatac gtgtcgcttc tagttttttc agcgcatcct
    34921 ctccattcaa tgcggtgatg actgaaaagc ccactcgttc caacacagaa cggtaaagca
    34981 actgtaattg cggatcatct tctactacta aaatacgatt cattgatctc cctctgtttt
    35041 ttgaaaaatt tctagatttt ttcttgtagt tcatcttcag ttaacattta tttcgtatac
    35101 tacgatcatt gttctgatat acttattagt ataccgaata tttcaatacg ggagaagggt
    35161 gattatgctt caattaaaag atataaaaaa atattataaa gtcggcgaaa ccaccaccaa
    35221 agcattggat ggtgtgtctg tcgctttcag acaaaaagaa ttcgtagcga ttttaggtcc
    35281 tagtggttct ggtaaaacga ctatgctgaa tgtgattggt ggactagata attatgattc
    35341 cggtgatatg gtaatcaacg gaaagtctac taaagacttt aaggacagtg actgggatgc
    35401 ctaccgaaac aactctatcg gtttcatttt tcaaagttac aacttgattg gtcatctagg
    35461 cattattgaa aacgttgaac ttgggatgac attaagtggt gtttctaaag acgaaaaacg
    35521 aaaacgggca gaagatgcat tgcaccgagt cggcttgaca aatcatatgc ataaaaagcc
    35581 aaatcagtta tctggtggac aaatgcagcg agtggccatc gctcgagcac tagccaatga
    35641 cccagatatc cttttgtgcg atgaaccaac cggagcgtta gatacagaaa caagtatcca
    35701 gatcatggaa ttgatccaag agttatcgaa agaaaaatta gtgatcatgg tcacacataa
    35761 tcctgaactg gcccatcaat acgcagatcg aatcattgaa ttttctgatg gtaagatctt
    35821 aacagattcc catcctcaca tcgaacgacc aaaagatgat caatttaact tacgcagaac
    35881 aaagatgagt ttttggacag cgttaaagct ttccttcaac aatatccgaa caaaaaaagg
    35941 acgtactttc ttgacctcct ttgcttccag tattgggatc atcggaatcg cgatcgttct
    36001 gtccctttct tctggattcc aaaaacaaat cgataacacg caagctgaaa cgatggcgaa
    36061 atttccgatt actatttcta aggtaacgac taaccagact cgtgacgatg ccggattggg
    36121 tgcaagcaaa gcggattatc cagacagcaa aacgattact gcaaaagtca gcgaagaaga
    36181 taaagcacag cacacgaata agattgatca aacatacgtc gattacgtaa cagacatcga
    36241 tcctaactta agtaataata tcggatttac ccgtactaca ggaatcaatc ttttacgtga
    36301 tgtaaatggc aaagtacagc ctgtcagctt ctcgaatcaa aatcctgata cggaatctct
    36361 ttctttatct agtacgatgt cagcaatgac aggtgtggga gtgtcaagtt tccctacaca
    36421 gcttgacact agcaaagaaa actttttaaa agacaattat tctttacttg ccggaagtta
    36481 tccagcctca gcaacagatg tcgtgctgat cgtagacggc aataataata ctaatatcaa
    36541 cgcattgaag aatcttggat ttgatgtgaa agaagacgaa aaacttgatt ttgatgatat
    36601 cgttggtaca acattcaaat tagtaaacaa caatacttac tatacaaaac ttccaactgg
    36661 gaattttatc cctaatacag attatgatgc gatgtatcag aatgcttctg atgaattgaa
    36721 gatctcgggg atcctacgcg tgaaatcttc ttctacaatg aatctcttat ctccaggaat
    36781 cgcttatagc gatcaattaa cgactcaaat cgtcaacgaa aacaaagaat cagaaatcgt
    36841 caaagctcaa agagacagtg gtgtaaatgt cttaactact gaaaaagttg acgaaaatgc
    36901 caaacaaaca ttgcttagtt atttaggtgg agacagcttg ccttcaagta tcatgattta
    36961 tccgaacaat tttgaagaca aagaaaagat cttggattat ctagatgatt acaataaagg
    37021 caaatcagat gaagataaaa tcatttatac tgatttagct ggaacgatga ctgaactgac
    37081 tggcggattg atggatgcta tcacttatgt ccttattgct tttgcaggga tctctttagt
    37141 aacaagtatg atcatgatca gtatcattac ttatacatct gtgattgaac ggacaaaaga
    37201 aatcggtgta ctaaaagctt taggtgcacg taaaaaagac atcactcgcg tatttgacgc
    37261 agaaacgtgt attttaggta tttcttctgg gatattaggt gtgtttattg catggctagc
    37321 tacgttccct atcaatagca tcttatacaa catgacggat ctaaaaaatg ttgctcaact
    37381 aaatcctgtc catgcgatta ttctagtgat cgtctctact atattgacga tgcttggcgg
    37441 tcatcttcct gcacgaatgg cagcgaaaaa agacgccgct atcgctttgc gtgcagaata
    37501 aggttgtgac aaccacattt agatttgagt cataagagcg ctgaaatagt agaaaacagc
    37561 aagaaacgag gaagtaatat gttccttgtt tcgcgctgtt ttttttaatt attttttata
    37621 attctggtat acttttcttt cctttgtacc acaactcttc tggcgtatac ctgtcttttc
    37681 catttatgaa tactgttcca tctgcttcaa tcgttacttc agtcccaatc cacatattac
    37741 gagaaagtgc gagaagattt tttctttttc ctcttccttt ttttccaaat tgcaaggcat
    37801 cggttatctc ttctgtccaa tgagatttca cgtattttac ttctccgttt tcaaatgtaa
    37861 ctttcaaaat attcgctata ttttccactt ttactgcttt aggaaatgac tcctccattt
    37921 ttttcacttt ccttcctcca acactaagta tgcgctttct tttagtgata agtatatcat
    37981 agtttattct attatcaaaa aaacgcatcc attccattgt agaatacttt accgaacaag
    38041 ttgaattgtt ttcctctttt tcacctttta ctatagatag gtaaagacaa ctaaggagga
    38101 atctctcatg acagatcaac cgtatgattt aagaaaagtc atcgaagaac taaaaacact
    38161 tccaggtcaa tatcacgaaa cagatattga aatcgatcct gaagcagaat tatctggtgt
    38221 ttaccgttat atcggtgctg gcggtacagt aaaacgtccg actcaagaag gaccagctat
    38281 gactttcaac aatatcaaag gtttccccgg cacacgtgtg aacatcggaa caatggctag
    38341 tcgtaaacgt gtaggtgaaa ttttgcacca tgattatcga acacttggtc gtttcttgaa
    38401 agacgctgta gaaaatccaa tcaaacctgt gaaagtagac aaagcagacg ctccagcaca
    38461 agaagtcgtt catctagcta ccgatgcaga cttcgatatt cgcaacattt tgcctgcacc
    38521 tacaaatact cctgaagatg ctggtccata catcacaatg ggtgtagtct taggatcaga
    38581 tccagaaaaa acaatgagcg atgtaacgat ccatcgtatg gttctagaag ataaagatac
    38641 gatcggtatg tacatcatgc ctggcggacg ccacattggt cacttccaaa aacaattcga
    38701 agccttgaat aaaccaatgc ctatcactat caatattggt ttagatccag caattgccat
    38761 tggcacgact ttcgaaccac ctaccactcc acttggctac aatgaactat ggtgtgcagg
    38821 agctttgcgt aacgaaccta ttcaaatcgt tgatggtata gcagtcgatg aagtgggtat
    38881 tgctcgtgct gaattcatca ttgaagcaga aatcatgcca aatgaaacaa tgcaagaaga
    38941 tatcaacacg aactcaggaa aagcaatgcc agaatttcct ggctatactg gggatgctaa
    39001 tccagctgta caagtagtga aagtcaaagc agttactcac cgaaaagaca atccaatcat
    39061 gcaaacaacg attggtccca gtgaagaaca cgtatctatg gccggtattc ctacagaagc
    39121 aagtattctt gccttggtcg ataaagctat tccaggaaaa gtagtaaatg tctacaatcc
    39181 tcctgcaggt ggtggtaaat tgatgaccat catgcaaatc cgtaaagaaa acgaagcgga
    39241 tgaaggtatt cagcgtcaag cagctattct tgctttatca gccttcaaag aattgaagac
    39301 agtcatttta gtcgatgaag atgttgatat tttcgacatg aacgatgtag tttggacttt
    39361 caacactcgt ttccaaggag ataaagatat catcgtgatc ccaggaatga ggaaccatcc
    39421 attggaccca tcagaattac cagaatataa tcctggctct attcgtttcc gtgggataag
    39481 tgcaaaaact atctttgacg gtacagttcc tttcgatatg aaagaacatt ttgtacgtgc
    39541 aaaattccaa gaagtagaag attgggaaaa atatttgaaa taatgaatga tctgcaactg
    39601 ttttgacatc ataaaaagga gtgcggaaaa tggaacaatt ggcacaacgg aagaaaaaaa
    39661 tcgtgattgg tgtaagtggc gcttcaggca ccatttatgc catcaaccta gtgaagaaac
    39721 taaaagaata tccgatgata gaaacacacg gaatcatcag tccatgggca aaacagaact
    39781 taaagctgga atccgactta tcactaactg aattcgaaag cctgtttgat taccattatt
    39841 caaacaaaga tttaggtgct gctgtcgcca gcggatcatt tttgaccgat ggaatgatca
    39901 tcgtaccggc aagtatgaaa acagtcgcag gtatctctgt tggtttaggc gacaacctga
    39961 ttgcacgtgc tgctgatgta acaatgaagg agcaacgaaa gttagtcatt gttcctcgtg
    40021 aaacgccatt aaatgccatt cacttggaaa acttgactaa attagcacga ctaggtgtgc
    40081 aaatcatacc accaattcct gctttttata atcagcctca aacgatccaa gatcttgtta
    40141 atcaccatac aatgaaactt ttggatcaat ttgaaattgt aaatgagcat tcaaaacgtt
    40201 ggcagggtga ttaatatgga aaaagaaacg atagttgaag taaccaaact aggtttcacg
    40261 atccaagctg ctgttaaagt aatcggtaaa gacttattga tcactttaac aggcggagat
    40321 acaccacata tcggaacagt cactacattt tctcgtgata cagagatgca aactgtacgt
    40381 ttccctagcc acgatggtcg gttccataaa gacgatgtct tagccgaaaa aatagctgtg
    40441 atcatcaaaa ctgccgttcc gggaagttgt acgatcactt ccggtatcca tgttgaccat
    40501 atctccaatg cacaaatcaa cgcctctttt ccgatgtcag aagagttagg ttacaagatt
    40561 ttagattggt tggaaaaaat agatttccat atcaaagaac cagtttatta taaagatggt
    40621 gaaaaaccat tatgaaagaa gaggttgtga aatcagcgtt atgacctgag tatgagagaa
    40681 aaaaatggaa aaatagcttc tcatattttg ccgtttttga gctaataccg agggacagct
    40741 gattgaacgc cgtttatcaa gaggttgtga taaagccgct tcgtttcgag tagtaagaaa
    40801 aattttgtaa acatgggttt ccacattttt gcataaattt gacttactac cgagaaactg
    40861 gcttttgaac accgtttatc aagaggttgt gacacgtctt ttgtcacaac ctcttttatt
    40921 tttgcttcac tgtaactgct acaatatcac cgatttcttt tccgatgttt ttacgaatat
    40981 cttttcgtag tccaaggatg tagcagactg aaccatctgg atttttcacg cccatattca
    41041 cgatactgcc ttcatacggc acattgtcaa aagttgcttg gactttaact cttccagcac
    41101 cgaattcttt tcttatatca tacggaaaaa taatatatgc cccgcccttg ccgacttcgg
    41161 aagcatagat ttttgattca aaagaataaa tttccctcat tttatttctc ctatttttta
    41221 catctagtat agcatagcaa aaaaaagaag gaactgtgac aaaagtcttg tcgcagttcc
    41281 ttttttcgaa taaacggtgt tctcctgcca actcctcggt cacaagtcac aatagtcaac
    41341 aaacatgaga agctgttttt tgaaattgct ccttgtgact cagagttaaa cggcaggctc
    41401 acaacctctt ccttaaacgg tgttcaaaag cttgcatcta cggcactaag cccaatcaga
    41461 tgaaaaatat ggagtgctat ttttatctga ttgttcttac tgcttgatgc agagcagctt
    41521 tttcacaacc tcctacatgg tgttcaaaat attattcgcc ttttgcttct ttcactaatt
    41581 ttgcgaatgc ttgttgttgg aattcagcag ctaaggcttt cgtacgttgt tcttgctctg
    41641 ctttgctcat ttttggattt tcagtcattg cttttaccat accgtcttga acataagctt
    41701 ttgcttcact ttccatcgta ctttgctgtt tcttcactaa ttcagctaac ttggctgctt
    41761 ggtccgtact taattctacc catgtctctg ggattttgaa tgtattcact cggctgtcat
    41821 attcgtgatt gttatcagca atattgaacc agaatttata catgtcgttt ttgtattccc
    41881 aataagtagt tttcgtcact ttttcagcat cgccgtctgt cttttctact ttatttgttt
    41941 cgttatccgt accagttgct tttggttttt cttgtttttc atttgttcgg taaagataca
    42001 ctttttcttt tccattccct aaagctttat acaaaagcat atctgctcct tgggaatcag
    42061 cactggaaac aattttttct gtttttgttt ccgttacttt ttccattcca aagtgattgg
    42121 acaaattagc gacgatcaaa cttagtgaag cgacgaagat tagtccgaaa atcaaagata
    42181 aaaaggtttg ccatgttttt ttagcgaaca cgttcgttaa agcaaaagca agcaagctga
    42241 caatcaaaac tccaataatc atgcattatc ccccgctttc attagaacag gttctttttt
    42301 cttcaataaa aaggcaataa taaagccaag aatcccaaag atgatcgcta cgataaacgc
    42361 ggcatggtaa cctgacagtg tggcattgat tgcctgatct ttataagcta atggcgtatt
    42421 atccaataca gatttgcttg ggatatttgc ttttgtcaca ttcgtcaaca cactgatcaa
    42481 aatagctgta ccaatcgagc ttgcgatttg acggaatgta ttatttaccg ctgtcccatg
    42541 actgatcaag ttatttggca atgcattcat accagaagtc gttaccggca tcatgaccat
    42601 ggagatccca aacatacgga tcgcataaag gatcacgata tataaaactg gagtcgtttt
    42661 tgtcaagaaa gcaaatggtg cagttgccgc tgtcaaaatg aacatccctg tgatggctaa
    42721 acgtttcgcg ccgtatttat cgaaaatcgc acccgtcaca ggcatcatag cccccatgat
    42781 caatgcaccc ggcaacaaca tcaagccaga atggaatgct gattctccac gaatattttg
    42841 aatatacaat ggtacgacca tttctgcccc taccatcgcc atatttgtta caccagctaa
    42901 aacagctgca atcgtgaatg tagatgattt gaatacacga agttccaaga atggatgttc
    42961 caaatgtaat tgacgccatg agaacaaggc gatgaaaatc acaccaacaa ctaagaaacc
    43021 aattactttc aagcttcccc agccgtcatc tcctacacta gaaaatccgt aaagtaaact
    43081 accgaatcca atcgttgata aaatagcgga taaaatatca atgctaggat ttgacaatgg
    43141 aataacactt ttcattagga agaatgctaa aaccaagaca agaacaacga ttggcagtac
    43201 cataccaaac aagtcacgcc aatggtagtt atcaatgata taaccagata atgtagggcc
    43261 taaagcagga gcaagtccaa taacgatccc tgacatcccc attgctgatc ctcttttttc
    43321 tggcgggaaa atagatagca tgatattttg caataatggc atcgatactc ccacaccgca
    43381 agcttggatc aaacgaccga ttagtaatgt actaaaatta ggtgctgtaa agcaagtaat
    43441 cgttccgatc aaaaagatcg tcatggcaga aagatataaa tttcttgaac tgaatttatt
    43501 gatcagccat gctgttattg ggatcatgat cccgttgact agcaagaaac cagtcgttaa
    43561 ccattgcaca tcagacgctg aaatatcgaa agctttcatc aatgtaggaa aagctgtcgt
    43621 caataaggtc tgattcaaaa cggtacaaaa tgtaccaatt aataagacaa caactaaaag
    43681 tgtccgatta tatggttttc cgtaaatatc tacgggttgg ttgttactca tatttctcct
    43741 tctttctgaa ttttttctga aactactaga atactttagt cctaaattcg ctatttgtca
    43801 acctacttga cacataagtt aatacggata taattaaggg aagcaagagg acaaaaggag
    43861 tgacaaaaat ggttggggct tcccttaaac gtttgttgga aaatgatcaa ttggttgttg
    43921 gcatcagtga attaagtgat atcgtaggtg tgtctccacg tcaattaagg tattgggaac
    43981 aaaagggatt cattcgttcg atcgaagctg atgcgaattg tcctagaaaa tatcgtttac
    44041 ctacagtcat aaaagtagaa ctgatcaaaa aatatttaga tgacggattt actttgtcaa
    44101 aagctgcaga aaaggcagaa atccgccaaa agaaaatgca ccatgtccgt aaagtttttt
    44161 caaaagcaat caaagatatc gaattgatcg acgaacgcta tacgatttta caaatcggta
    44221 acttcaaaga agagaatgct aagatgtaca tcattcatga caacgagaca gacgaattaa
    44281 cttacaagct ccttcctgtc gatgaaaaag ctgactacga acagttgtta aaagaactct
    44341 aaacgccact aatctcctaa gtaccaagct ttttaataag tagatgctac ataaatactt
    44401 gaaaaaaggc ctgtttgaac ataagaatat ccgaacggtg atctgcgttt ctttcatata
    44461 gaaaggaatt ctccatcact gttcggatat ttttttattt ttttcggtta ctttcttcta
    44521 atacatctgt tagccaattt gcatattctg tttcccgatt gatcgcatgc gctaaaatca
    44581 ggtagtgtcc gtaattttca tgtttttctt tttcagtagg aaagatcgtt ttttttcgct
    44641 ctaatagatg aagtcgtttt tcttcatgaa ggtcacgctg ctgctgaata atttctacta
    44701 atgccggatc attcacgtct ttgacgaaat acaatttcag aacaaattcg tctcgattaa
    44761 ccggtagttc attggtgggt gtatgaatcc actctgccaa ttgttccttc ccttcttctg
    44821 ttaattcata aactttcttt tccaattttt cacctgtgat ctctacttga tgctgaatga
    44881 ttccttgctc ttccattttt gcaagttcag gataaatctg actatgtttt gcttgccaaa
    44941 attctcctat ttcatttttg aacgattgat tcaattcata tcctgtcatc ttcttcttac
    45001 ttaataaccc taacaaaata tatcgaagtg tattttttct tgccatcagt tttccctcct
    45061 tgacaaatct ttaaatccat tctatcataa atgagtaatc gaaaacatgt agtttattac
    45121 atgttttaat ttataaggag atgcttaaac atgaaagaat ttaaagaatt ggaagatttt
    45181 attggaactc actttatcta tacgtatgac aatggttggg aatatgaatg gtatgccaaa
    45241 aatgaccata ctgttgatta ccgtatccat ggcggaatgg tcaaaggccg ttgggtaaaa
    45301 gaccaagaag ctgatatcgt taaattgacg gacggcgtat tcaaaatcac ttggacagag
    45361 ccaacaggga cagacgtggc attggacttt atgccaaatg aaaagaaact tcacggaatg
    45421 atctttttcc ctaaatgggt tgaagaacat ccagaaatca ctgttacttt ccaaaacgaa
    45481 catatcgcag aaatggaagc agctcgtgaa aaatatgaaa cttatccaaa attacttgtt
    45541 cctgaatttg ctacgatcac ttatatgggt gatgcaggta tagacaatga agatgttatt
    45601 agcgaagaac catacgatgg aatgccagat gatatccgta gcggaaaata cttcgatgaa
    45661 aattataaac gagtaaaata aggttgtgaa agagaggttg tgaaatcagc gttctgacct
    45721 gagtattaga gaaagaaacg gaaaaatagt tcctcatgtt ttgccgtttt ttgagctaat
    45781 accgagggtc agctgattga acaccgttta tcaagaggtt gtgaaatcag cgttatgctt
    45841 tgagtaataa gaacgctagt tagaaaatag cttttcatat tttctaggct agtgggctta
    45901 ttaccgagaa gcagccgttt gaacaccgtt tatcaagagg ttgtgaaatc agcgttttga
    45961 cctgagtatt agagaaacaa atggaaaaat agttcctcat attttgccgt tttttgagct
    46021 aataccgagg gtcagctgat tgaacaccgt ttatcaaaga ttgtaacaac tgcatattca
    46081 cacagttaaa ttagtgactt caaaatgaaa taatgctccc agcaaaaaat taggctggga
    46141 gcattatttc gttattctgt tcatcaaaaa tcagcgtttt gattccattt catcacaagt
    46201 tcctcctcat ttactgcttc atctttttgc tgtgtttgaa ttgtaaaacc ttgtgaaaga
    46261 tagaaatgaa tagctgattg atttttttga tagactgtca aagtcagtgt tttattggtt
    46321 tcttttattt ttttcattag ttgcgtaccg attctttgtt gccggtattc ccttttcaca
    46381 aagattcctg cgataaactg atcaatcacc ccagcaaaac caataatttc ttgatctatt
    46441 tctgctaagt aaatcactgc atctggtaaa gctttcctaa cagattctac ttgattctcc
    46501 caataagttg atggaatgaa ttcatgagaa tccaagttag catgcagcca aatcgataga
    46561 acagattcca gttctttggc agacaatttt gttgttgtgc ggatcatttt ttctctcctt
    46621 acttattcat tgattttaaa ataaaaacca tttttttgaa gctcttttac aagttttcgc
    46681 ccctcaaaaa agtatatctt ctcttgtttt acttttctaa taccaaaaga aacagcaact
    46741 ttaggatcat cagctgaaac gattcgataa gttccaccag gcaattgatc cagcttgact
    46801 ttcccatgat ggtcgctttg tactgaaaac ggcacaccat ttctagtaag tgtttttttc
    46861 ttatgtttca aaatgaatga actaccattt accggtttcc ctgacgaatc actaataata
    46921 aatgaaaaat caagcacttt tttacttaat ctatactgat agattagtcg tccgataaac
    46981 actaacagta tgactacagc tattatcgtt cctgcaagaa taaaccattg ccgcatggtt
    47041 ctttcctgat ttccttttcc ttcttctttt tttaccgatt cgttataagg aatccgatgt
    47101 cctgtcacta gcaaacgatg cgtgttgatc atatacggcg tacatgtaag caaggtaact
    47161 aagtcttctc cttctgaaat cgttagaaca gacgtgtcat ctggcagtac cacttgtata
    47221 tttttaactt gataagccaa tttctccccc aaaacagtca aaataaacgt gtctccgttt
    47281 tctaaacgat ctaattcggt aaataaccgt cgattaggta aaccgctgtg agcagagatc
    47341 actgaatgcg tcgaatttcc accaagcggg aaagaggttc cttgcaagac gcctgctcca
    47401 ttttcaagga tcctaccatt gagccgatca taaagtgaaa tatttaattg gatcttaggt
    47461 atattgatac ttccgatcag ccattcctct aattggctgg atgttgtact ttcatttctg
    47521 ttcttttcat tgaaaggatc agtaccagga ttgaatcctt ggtcagttaa tcgttggttt
    47581 ttctcctcca ttttctttcg ctgcacttca atttcagctg cggtccgttt ttgtacttgt
    47641 tcgatacgtt tttgatcaat aaaactattt aaagaatcca cataaaatgg atataaagcg
    47701 atcagtaacc ctacgacgat acatattaat aaaaacaaac gaaacaaaag tcgacgagac
    47761 tgtttttgct tactctgctt cacatgtgtc ctcccttgca agtttttttg ttctttcttt
    47821 tattatatgg gttcaggaaa aaaatagaaa atgaaagccg atgaggttgt gaaaaagctg
    47881 tcctgcatca agtaataagt acagcccaaa aaaatagcta ctcatatttt ttgttgggtt
    47941 gggcttatta ccgcagatgc aagcttttga acaccgttta aggaagaggt tgtgaaaact
    48001 gcgttatgct ttgagtaata agagcgctag tcagaaaata gcttctcata ttttctaggc
    48061 tagtgagctt attaccgaga agcagctgat tgaacaccgt ttaccgagag gtcatgaaaa
    48121 agctactttg tttcgagtat taagaaaaat tctgtttcaa aacaaggaga tgcaacaaaa
    48181 cttatgtcgc atcctctctc cttagaactt tcacttatgt ttttcttgta aaattcataa
    48241 atattttcat gaattttcta atttcttgcc tacctctcgc ataggatata tgatgaattt
    48301 atctaaagtt tgcaaaggag ttcgacaatt gaaaaaatat ggcccatttt tagcaatttt
    48361 tttcattgct ttggcatttc tcatgcctca tttattcaca cataaactaa ttttgggtgc
    48421 tgatgcccca ttccattaca atcggttcta tgatgccgcg caacaaatta aatatgggga
    48481 tttccattat tttccttccc tttatggatt ccagcaaagt ggcaggatcg tgaatgccgt
    48541 ttacggccca tttttcgctt atttacaagg attgatcgtg ttgcttgcag gtagctggtt
    48601 tcattatcag attctttcaa atttcattct ttatcttatt tcaggatttt ctatgctgat
    48661 tttactgaga tatgtcaatg ttcgaaattg ggtaagtgta ccagtagccg ttttattcat
    48721 gagtacgtat tctattcaat actggtctat caaccaagga ttcactagct gggggacttg
    48781 tttttatcct ttatgtatga tccctattgc tgatttcgtc cttcataaaa agttccctgt
    48841 tatcaaagtc gctgtctcag tgggacttat gacacagatc catttactgt cttcgatcat
    48901 gttagctttg atgtatatcc ctttttatct ttattacttt tttaaccaga ctgaaaaagg
    48961 aaaagcactg cttcaactga ttaagtcggt cttgctttat tttgttctga cagcaaatat
    49021 ttgggcagga ttagctattg tttacagtgg gaataagatt cttcctccct ttgttaacag
    49081 agaaatgagt caaaaggcga tagatctgga tggcagttat tggctccatt accctaaggc
    49141 ctttcctata ttgatattat caggtttttt gctttttatt ttctttcaac aaaagaacac
    49201 cttattccaa aaaatcgtgt tgtacacaag tcttgttttc ttttttcttt ctactagctt
    49261 gattccatgg acgacattga tcgaaaaaaa tgtgcctttt attagtttga tccagtttcc
    49321 atttcgattt ttcgtgccat ttacgctatt gcttctactt taccttgccc tttcactgaa
    49381 ccagtggtct gaacaaaagt ggttccgtct tttaagtata tttggcattc ttattttttc
    49441 agcacagacg atccagaact tataccagca tctggaaaag tgggaaaatg aaacatttgt
    49501 cagtcgacat acttatctat tcgatacgcc ggaagaagca aggaaaacat tcttcagcaa
    49561 agatatgtct accagcttgt tcgttttcca aaaaaccaca cctgactatc tgccaatcta
    49621 taaggagaca aaagacaata aatacgaccg gtataacgaa gaaatactag aaaacgaaac
    49681 aacttttata aaaacacatc caggcggtct tttgacaatc aagtggaaaa acactgggga
    49741 caaaactgtt catatccctg ttatcgtcta cgatcggact gtgctacagc aaaatggaaa
    49801 aacattgact gattatgaag taacagatat cgggacccca atcgtgaagc agcaaaaagg
    49861 aatcaacgag ctcacgcttc attatcaaac gccaatctat ttctatttca ttctttctct
    49921 tactttgatt ggctggttta ccttgctatg tttatttatt tatcatagat acaagctttt
    49981 acgtgcttag gcaaaaggga taaatacatc cgatcaaaaa actctccaaa cggtataatc
    50041 gtttggagag ttttatttta atcagttttt ttatgttata tgttatattt tttattcgtt
    50101 cacatctaat tcgatttcca ccccgtagtg atcactgatg attggactat cgatcccatc
    50161 aaaaatccgt tgatattttt taacctgcat tccttcaggg acgaaaataa aatctatacg
    50221 caatttttct gtattttgtt cccaaccatc tattttcttt tccacagtag ctgcttttcc
    50281 tttttcattc gcgacaacat acgaatcttg aagaagaagg caactttctc ttacagtata
    50341 ataaccttga gagtctactg gattattgaa atcacctagg aaaaaaagtg gtacgcgagt
    50401 ttccaaaaag tagttttcca agtttttcca ctcaaaataa aagccacttt ttttatctgt
    50461 ccaccaagaa aagtggcagc tcgagacaaa aatgtcctga tcatctatct ttgttttccc
    50521 aatcaagatc ttgcgtgtct gataatcttc tttcttttga aattgggaga cggtaaggac
    50581 ctgactttcc aatgtttttt tcgtaagcag tgcatttcct tcttcatatt tatcataacc
    50641 gatgtggctc atttcccaac tccaaaaata ctcttttccg tgttccttta aaagctgaac
    50701 taaacggtag gcaaaattat ctttacggat aggtacttga ttctttaccg gacagaactt
    50761 cattagcttc gcaggttcta cttcttcaga ttccagtaat tggttgattt cttgtaaagc
    50821 aatcacttca taatcttcta acaggatctt atctgctaat tcttgcaatt ttttctctgg
    50881 ttctggctca agccagctat gcgtgttgag tgtcatgaat ttcattggtt ttctccttgt
    50941 cttcatttcg aaacaaaagg gttgtgacaa aatttttgtc acaacctctc cagcaaatag
    51001 tgttctcctg ctaactcctc agtaataagc tcaatggacc aggagctatt ttttgtttgg
    51061 ctgtttttat cacttggtgc agggcagctt tttcacgacc tctccactaa acagtattca
    51121 aactgctgct tctcggtaat aagcccaacc aaacgaaaaa taggaggagc tatttttgtt
    51181 tggctgttct tatgacttga tgcaggacag ctttttcaca acctctcctt acattttaat
    51241 ggagccgatc actttaccag ctgtcactgt acctaattgc ttgatcacta cttcttctac
    51301 tttatcgttt gtaaatacga tcatgatcgt tggatctttt tcttcgtttt ggatcacatc
    51361 aagatccatc tcagctaatt tcgttcctgt tcctacttct tggccttctt tgacagcaat
    51421 ctcaaaagct ggctgattca tttggactgt atcgattccc atatggatca aaacttcgat
    51481 accatctttc gtttccagcc caattgcatg tttgctaggg aaaatacttg tcacttttcc
    51541 agcgatcggc gcaacaactt ctcttgatgt tggaataaca gcaaagccgt cacccatcat
    51601 tttttgtgag aataccggat cttttacttc agataaagga atcacttctc cagaagctgc
    51661 ggaaactagt tcttgttcta tacctaagta ttgcttacta cctgcttgtc catttcctgt
    51721 agaaaccatt tctggctctg gaatctctgc tcctgattgc agcagatctt cgatatctga
    51781 tttcaagaca tctgcttttg gtccataaac tgcttggact ccattgtctt tgacgatcaa
    51841 tcccatcgcc cctgctcgtt tccaggcttc ttcagtccct actttttctc gatcatttac
    51901 acttacgcgt aaccgagtca tacaagcatc gacatctttg atattttctt ttcctcccag
    51961 caaatgaatg atctggacca cttgctgatc tgctgttcct gttccgctgt tagatacaac
    52021 ggtttcttca ctgtcattgt cgtaatttcc gttacgtccc ggtgtagcaa agttgaattt
    52081 tttgatcaag aaatttgcta agaaatacgt aacaacacca aaagcgatca cacacaacac
    52141 gaagttgata agatctccac ctagacccgc tttgatagct aacggtgtcc gagtcagcaa
    52201 ttcgatattc ccaaatgaat ggacccgcaa tggcaagata tctgccatcg caaatgcagc
    52261 cccttggatc actgaataaa tgacatataa tggcacagca gcaaacataa acatgaactc
    52321 caatggttct gttacacctg ttagaaagac agccaacgct gctgaaaaat acattgattt
    52381 atatcttgct ttcttatcag gatcgacatt acgatacatc gcaaaagcca ttcccatcaa
    52441 gatacctgaa gaaccaatca tttgtcctac tttgaaacga gccggcgtcc agttatgcaa
    52501 cacaaactca tattgcgaca tatcccctgc acctttaaga ttcactaaat cagtggccca
    52561 agccaaccat aacggatctt gcccaaatac ttgtgtcccc gcctgtgcgc ctgataaaat
    52621 ctcatacgat ccgccaagct gtgtatagtt gataggaatc gtcagcatat gatgcaatcc
    52681 aaatggcaat agtaaacgtt ctaatgttcc aaataaaaac ggtgctagaa tcggtgcgct
    52741 ttcttgtgat tctgcaatcc ataaaccaaa attattgatt ccagcctgta cgtttggcca
    52801 caccacagca agaatcaatg ccacgattgt tgaccataag ataacaacaa acggaacaaa
    52861 gcgctttcca ttgaaaaatg acaaagcatc cggcaatttt ctataattat agtacttatt
    52921 ataagccatt gccccgacaa atccagcgat gatccctacg aatactccca tattcaaagc
    52981 tggtgcttcc aatacacttg taaaaaatcc tttaaccatg atcttcgtac cgaacagagt
    53041 gtgggtaaat gcatcttcat tcgcaagcat gtcacttgtg acaccaaaga tagccccagt
    53101 gatccgattg attaaaataa atgagatccc agctgcaaat gctccgcctg ctctttcttt
    53161 tgcccaactt cctcctattg ctaaagcaaa taaaatatga agattaccaa taattgccca
    53221 accaatattt tcaattactc cgcccgttgt gacaagcggc gtccaatcag gattgatcaa
    53281 gggaattgat ttccctatac tgatcatcaa cccagctgcc ggcataactg ctaccacaac
    53341 cattaaagcc tttccaaatt tttgccaaaa ctcaaaacta aatatctttt tcattccgtt
    53401 tagctccttt acgcaatcgg tttcgttaaa cgtattatag cgtgcgtttt cttttttgta
    53461 aatgctttat tttttatttt tctgtctata ccattctata aaacctataa tacgtgtgtt
    53521 cccttctata attttctcct aaaaatttat ttttttaagc ttttttctat gaaatatttt
    53581 cttttgtcta ttgacttgtg caatcggttg cgtttattat gaacgtataa aacagctcat
    53641 taggaggaga aaaatgaaac agatcaaacg actatttgct attgatccat ggaaaatcag
    53701 cacgacacaa ttagataaaa aaaatcttcg actgcaagaa tctttaacaa gtattggaaa
    53761 cggttatatg gggatgcgcg ggaactttga agaaacttac tcaggggacc accatcaagg
    53821 tacttatttg gcgggtgtct ggtatcccga taagacacgt gtcggctggt ggaaaaatgg
    53881 ttatccagaa tactttggaa aagtgatcaa cgccttgaac tttatcgcga tagatatttc
    53941 gatcaacgat cagctgatcg acttggcttc tttggaacca gaagactttt actgggaatt
    54001 agatatgaaa aatggtgtat tatctcgtac attcacaatc actacttcta ctaataaagt
    54061 acgcatttcc ttcgaacgtt ttctgagcat tgtcaaaaaa gaatcagctt atatctattt
    54121 aactatagaa atgcttgaag gtagcggaac agtcaaactc gtatcaaaat tggatggaaa
    54181 tgttcaaaac gaagacagca actatgatga acatttttgg aatgagattg atcgtggaac
    54241 tatagaagaa atcggctatt tgacgactaa aactattcca aacaactttg ggattgaaca
    54301 atttactgtt actgcagcaa tgcgccattt cgttaatgga aaatcagtca tccctgacta
    54361 tcacgaagat actttagcat tacatggttg cttgtctgca gaattatctc aaggtgagac
    54421 atggtcagtg gaaaaagaaa ttatcgttct tactagtaga gatattccag aagcgcagca
    54481 agcagaaaaa gctgtctcag aattaacgaa ccagacaaac aattatatcc aagcaaaaga
    54541 agagcaaaca gcggcttggg cagaacgatg gaagcttgcc gatgtggtca tcgaaggaga
    54601 cgaagaagca caacaaggta ttcgattcaa tttgttccaa ctattctcta cttactacgg
    54661 cgaagatgaa cgattgaata tcggccccaa aggattcaca ggagaaaaat acggcggagc
    54721 cacttactgg gatacagaag cgtatgctgt ccctctttat cttgctttag ccaaacctga
    54781 agtaactaag aatctgctga aatatcggca taatcaactg ccgcaagcaa tccacaacgc
    54841 tcagcaacaa ggattaaaag gcgcgcttta tcctatggtc accttcactg gtgtcgaatg
    54901 ccataacgag tgggaaatca cattcgagga gattcaccga aatggcgcga ttgcctatgc
    54961 aatctacaat tatacgaatt acaccggcga tacggaatat ttgaaaaatg aaggactgga
    55021 agttttagca gaaatcgcac gcttctgggc agaccgtgtc catttttcaa aacgagcaaa
    55081 taaatacatg atccacggtg tgaccggccc aaacgaatat gagaacaata tcaataacaa
    55141 ttggtataca aatacgatcg cagcttgggt cttgcgttac acgcgagaaa attatttaac
    55201 ttatcaaaaa gaagctgcta tcactatcag cgaagaagaa ctagccaaat ggaaagatat
    55261 cgcagagaac atgtacttcc cagttgacga agaattagga atctttgtcc agcatgacac
    55321 attcttagat aaagatctca tgcctgtatc cgatattccc acaagcgatc ttcctctgaa
    55381 tcagcattgg tcttgggaca aaatccttag atcttgcttc atcaagcagg cagatgtact
    55441 acaggggatt tatttcttca atgatacttt tacaaaagaa gaaaagcaaa aaaactttga
    55501 tttctatgaa ccaatgactg tccatgagtc ttccctttcg ccaagtatcc attcgatttt
    55561 agcagcagaa ttaggaatgg aaagcaaagc tgtagaaatg taccagcgaa cagctcgctt
    55621 ggatttggat aattacaata acgacacaga agacggcttg cacatcactt ccatgactgg
    55681 tagctggttg tcgattgtcc aaggatttgc tcaaatgaaa actgcccatg atcagttgag
    55741 attcgcacca tttctgccaa aaacttggaa agcttattct ttccatatca attatcgcgg
    55801 acgtttattg ttcatctcag tcacagatga acaagttact ttcgagttat tagaaggttc
    55861 tcctctagaa atttacgtct atgaacagtc ttattcttta gcggatcgtc tagaaataac
    55921 gctaagaaag gaggaagcaa atgtttaaag gggtattgtt tgatttagac ggtgtcatta
    55981 ccgatactgc tgaattccat tatcgtgctt ggcgaaagct tggtcaggaa atcggcatta
    56041 cgatcgacca cacattcaac gaacagctaa aaggcgtcag ccgagaagac tcactctctt
    56101 tgcttttagc ttatggcgga aaagaacact ctttttcaaa ggaagaattt gcagaattag
    56161 cgaaaagaaa aaacgattat tatttagaga tgatccagac gatcgaacca aaagatgtct
    56221 tccccggggt cgtgccttta ctggattctt taaaagaaga aaaaatcaag attgctcttg
    56281 cttctgcaag taaaaacggg ccattcctac ttgaaaaaat gggtcttaca ccttactttg
    56341 atgctattgc cgacccggca gaagcaacaa acggaaaacc tgcgccagat atcttcctcc
    56401 ttgcagcaaa agctgtcgga ctaaaagcag aagaatgtat cgggatcgaa gacgcgcaag
    56461 ccggtatcca agccatcctc tcaagtggcg ctcaaccaat tggcgtggga cgtgctgaag
    56521 accttggaga ggagatcccg cttgtccctg atacgacttt tcttacgatc gattacttga
    56581 aagaaaagtg gcatgaccat ggataatcca gagatctccg tcgtaccagt tccagagact
    56641 gaaataaaga aaatcacatt gaagaacgac tacttagaag tcgttcttct caattacggt
    56701 gcccgcctgc accaaatctt cgcaccagac aaagaaggaa aatcagaaaa tgttttattg
    56761 tcttacgatc gtttcgaaga tgttctaaca gacaaaagct tttttggagc aactgtcgga
    56821 cctgtagccg gaaggatccg ggatgcttct tggggtgagc atagattgga gaaaaactgc
    56881 ggatcacacc atatccacgg tggcacgaaa ggctggtctt tccaattttg ggaagtagaa
    56941 gttttcaaaa ctccatcatc aataggtgtg gtcttttact taaaagatga attttctacc
    57001 tatccaggac caatcactgc tacgatcact taccgtttaa caaaaaacga attagagatg
    57061 acaacctctg ctagtagcct tgttgaaacc atctgcaatc cgaccaacca tgcctacttc
    57121 aacctttcag gaaatggaac aagagacatc tatgaacatc agttaacggt ctttttagat
    57181 ggtgtgcttg aattagatca agaaaaactg cctactggaa attggacaaa aaaagaaaaa
    57241 ctgccaatcg actttagaaa atccccaaca ctgcaagaga tccttgcatg ttatccagat
    57301 ggactagatg atgtatttct tttgcatcat cctaggcttt ccaaaacttc cttacaatta
    57361 ttcgagaagc attctggtcg ccaaatgact atagcaactt ctaataaaag tatggtatta
    57421 ttttctacga ctggattcga agctgatttc tctgtcaatg gaaaacagat gcattcaaac
    57481 tacggattag caatcgaacc gcaagaaatc cctgatatcg ttcatttccc caaatttgga
    57541 tcgatcaacc tccatcctgg tcaagaacgg atcagtcaga cgatttatcg gttctctgca
    57601 caataacaag agaaaggtgt ttttatcatg gctattaccg taaaagatgt ggcgaaaaaa
    57661 gccggtgttg ccacctcgac cgtctctcgt gtcatcaacg atcatcctag tatttccgag
    57721 acaacaaaga agaaagtccg aaaaattatg gatgaactag gttatgttcc taacatgact
    57781 gcccgcaatc taggaaaacg catttccagt gccatcggcg taatcttacc ccctcttgat
    57841 tcaaaagagc ggatcggtaa cccattttat cttgagatca tggaagcaat caacgaagaa
    57901 gcgcggaatt atgatatgac tacagcaatc gctactgcta aaaattttga attgctgtta
    57961 gagaacgtcc agcgtatgca tttacaaaaa caagtagatg gttttattct agtgtattca
    58021 gatagtcaag atactgtcat tgattattta taccaaaacc acatcccttt cactttgatc
    58081 ggacagcctt atcatcatga gaacgaaatc gtttatgtag ataatgataa ccagcttcta
    58141 ggtaaacaag caacagaatt tttgatccag aatggacata agaatatcct gtttgtgaca
    58201 aacacgactc atgaaagttt gtatttcgaa cgatatttcg gttatcaaaa agcaatgatg
    58261 atggctggtc ttcctgcgtt atccagtgta gtactagagc atccggagga ttatctgtcc
    58321 tttgagaaaa cgttgaaaga aaacgaagcc acagcattgg tggtcattga cgatatcttc
    58381 gcccttcgca cgatgcagtt ggcgcaaatg tatggctatg tcattccaga gacactctcc
    58441 gtcattagct tcaacaactc gatcttctca acattaacac acccttacct tacaagtatc
    58501 gatatcgata tctccgaatt aggacgtgtc gctatgcaaa agctgcgcga attgatccat
    58561 gaagaattga gcagtggcgt acgtttggtc atccctcatc gcttgatcaa aagagagaca
    58621 gtcgttcctt tagaaaaccc atactaaata gctgactcac taaatacgct gaccatttat
    58681 cctatggtca gctttttatg taaaagaaga tactacttct aacacattgt ttatctgtaa
    58741 aaaagagcgt gggacaaaag taaagaacac ttttgtctca cgctctaaac acgtataaac
    58801 ggcggaagca gaagcaactc cttcggaaat aagctgaaat tcacaaaaat ttaaaaagca
    58861 attttcttga gtttcttctt atttcttgga gttaaacgct tctgtcccag cctctttttt
    58921 ccttccttca attttttaca cgtcgacgat tcaataaaaa accgcctaat attgtaaaga
    58981 caggtgcaag aatcgagaaa aaggcgaatg gcaagtaatg caacgcgcta actttcatcg
    59041 ttcccataat gaatgtgccg ctgactcccc acggtacgag agaattgacg gtcgcacctg
    59101 aatccgctag tgttcgtgtc aaataggttt ttgataagcc aacgtcatca aaagaacgct
    59161 tgaatgtttc tcctggaaga atgatggaca gatactgttc tccaacgatc agattgattc
    59221 cgatacagct caaggcagta aacccgacta aacgagaagg tcggtccatc ttttctttta
    59281 attcttgaac gatggtatca acgatcagat atttgatcag taacccacct aatgccaatg
    59341 caagtaaaat caaactagca gaacctaaca tactcatgat cccgcctcga gaaagcagtt
    59401 catctagctt tggatcaggt gtatttgcga cataaccatt catcaagatg tctgctgctt
    59461 ttccgaccgt caaacttttc tcatggataa aagaaagaac gagagcagct gtcgatcccg
    59521 ctagtaatgt agggatagcc ggaatttttt tgattgcccc cccaaataaa ataactagtg
    59581 gaatcaatgt ccaaggagag atccaaaagc tcgctttcat gttttggatc atttcattga
    59641 tactagctag atcggctgaa tgactgttat gtcctaatat cgtataaaaa atcaacgaaa
    59701 ggacgaatgc tgggaaatcc gtataacgca taccagcgat atgttcgaat agattaactc
    59761 ctccaatacc cgctgccaag ttggtcgtat ctgataaagg cgagcagtta ttccctaaaa
    59821 aggcaccgga aacgactgcg cctgtagtca ttgcatcatt gaatccaagt aaatgaccga
    59881 tccccaaaaa agcaattcca atcgtcgaaa tcgttgtaaa tgaactgccg acagtcactc
    59941 cgacaattgc acaaacaaga aatgctgtcg gtaaaaagaa acgcacagaa acaagtttaa
    60001 atccataaac cataatcgta gggatggttc cagataaaat ccatgaagca actaagacac
    60061 caatcaaaag aaaaatcagg attggaatga tccctggagt gattcctttg atgatcccgt
    60121 catggatctc atcccatgtg aactttttaa atctgccata aaacaacaaa cacatgaaag
    60181 ctgcaaggat cgggatatgt ggcgagagct ggaatccgat gatcagtact cctaggagtg
    60241 ccaacaacac gatcaataca gcgatacttt cttttaaaga aatattttta ttcatcgtcg
    60301 atctcctatt tcaaatgaaa aggccctgcc atgaaaaaac tattcacttg gcaggacctc
    60361 ctactaaatc agcgacaaaa cgtcggctga aagacttatt tttttatttt tcttctaaac
    60421 ttttttctac ctcaatcccc caagaatctt tcttgaagtg attgatgatc aatggaataa
    60481 cgaacatgac cactgtgata gctgctacta ataatgcatc actcaatgtc gctgtaccta
    60541 atgtagcaaa tacgacaacg atgatagaga agatcagcat gcagctgact acccaagcta
    60601 atccgtttcc gtttttacca attcggtaag gacgttcgaa ttctggatgt ttcttacgca
    60661 ttttaagcaa tgaaacagca atcaaaatgt acacgataca ataaataaca gttgtagcat
    60721 ttgtaagtgt caagaataca gcacttacgt catccattac accataaagc aaagcaaata
    60781 atgaaataac gattgattgt gttagaacga catttctaga tacgccgtat ttgttttctt
    60841 tatggaatcc gaattttggt ggaaggaatc cttcacgagc tacttggatg attgtttttg
    60901 atggtccagt aacccaagct gataattgta aaagcacacc aataaatacc atgaagctga
    60961 agatatttcc gatcacttct ggtaagccta ggatttgaca gtcaatcaag ataggctgcg
    61021 tgatatttgc taattccatt tttccatctg gtacgacatt tgctacaagc atcgcattga
    61081 tgacattcaa taaaactaat ccgatcaaag cgataaatac gccttttgta taattttttg
    61141 ttgcgtcttt caaacgaggc atgtaaacag aactcatttc gatcccaaca aaaataaatg
    61201 tgataccagc taagtatttc aatgaatcaa tatgttccaa gtcagggaaa gcttttgacc
    61261 atgaaaagtc acctagatag ccgtttgaga ctagtccgac tttgaaggct gccaaaacac
    61321 ctaaaacgaa catgatgact actgggatat atacccctag ccatacaccg atgttcccgc
    61381 cgacttttgc catatcgaat ttcaagttca agatcgtgat gatccagtag ataactaaaa
    61441 tacaacctaa aacgaaccag tgattattgc caagttctac attaccaaat gtgtttccta
    61501 acaatggtcc taaagtagaa gcgaccatta ccatacctgg gaacatttgc acccataata
    61561 gccatgcaac gacaaatccc catttgctgc ccagtgctgt ttttacccat aactgaggac
    61621 cgccttcttg aggaagcatc gtggaaagct caccggaaac cattgatatc ggaccggcaa
    61681 aaaacaaaac tgcaaaaatt gaataaaaaa tcaatgtcca accgactgcg gctaacgtag
    61741 gaatactccg aaccgttgca cacagtgcca ttgtcagacc gataaaggtc ccaagaccca
    61801 ttttacttac ttcagattta ctcaccgttg acactctcct tgattttttg gataaaataa
    61861 cggtgtcttt gaagtagttt cagcaataaa cgaaacaatt cagcgaatat ttgaggggat
    61921 tctttgagtt tattgcttga agggtttctt caaagacaac ctcaaaacaa agggatcgaa
    61981 gagcttaggt ctccccttgc tcttcagatc caaacatgcg tcagaaacag ttagtattca
    62041 agctgttttc taacatgatt accttgctta ttttgcttcg cttgccaata attggtctgc
    62101 gtagattttt tccaattttt cttgaagagc tgctttgatt ttttcagcat attcttcaaa
    62161 gtgttcttct ttgttcatgt atggtgtcat tacgctggca cgcaatacag ttacttttcc
    62221 ggcacggttc cattcttcat ctgagaatcc tagttggttc acaaattgta atgggctgtt
    62281 gccatagtct gggatagcaa agtcagtatg tgaagttaag aactcattac cgtaaatgct
    62341 tcctttcacg tatgaagaat aatcatatac gtcatggttc aatttattca ttgcaactaa
    62401 gtcatcgttg cctttttctt tgaatacata gtctaccata ttgaaatctg gataagttaa
    62461 tggatgaact tcgatttctt tgtctcctac tttaaatgac aagtcattca agaagttgta
    62521 gaaacgatgt gcaccttcga ttgatgcacc cattaattta ccatatcctg tgacattcaa
    62581 aggtaagaca tggtgtgctg cccaaacgct ggctgctgtt gcccctgctt ttgaaccttc
    62641 caagatataa gccccaagta atgctgggat atctgcgcct ttttcaaata catatgtcgc
    62701 aaagtaagag ataacgtctc tcatgcggat atctttgatg acgataccac cggcagaata
    62761 tggaacatag cccattttat gtgggtcaat cgttacagat tctgcttctt cgattgcttt
    62821 atatgcactg tgtacttctt ctaagatata atctttgttt tctgtaaaga cattgtattt
    62881 gtaatgaaca tcttttaaat cttcaaatgg aatgaagttg ttgtcttcgt ctaagaaaat
    62941 cgcacggccg tatccgccat aagcagcatc tacgtgtaag tagaagtaaa taccgtcttt
    63001 ttctaatacg cgacgcaatg caacgatttt atcgataccg tcgattgctc cttcttctgt
    63061 tgatcctaca acaccgacta cacctaggat tggtgttttt tcagctgcta gaccacgaac
    63121 gattttttca agttcgttga tgtccatgcg atagttgtgg tcaactggca ctggaatcac
    63181 ttggtctaaa ccgataccga tgatgtcggc agcttttagc catgaatagt gtttagtttg
    63241 tggaaccaac cattttccta atttttctaa atgttttccg ctacgtgctg agtgagcttt
    63301 gatttcgtcg attttttctg ggacgctgtc taacagattc atgatttctt ctgttgacaa
    63361 gttcattaat tcccaatcgc tttttccagc aactagttca ggtgtcactt ctttcattgc
    63421 aagcggcaat gatttgatgt tgcgggcata ccaaagtcct tctaagtttg ctaaagaacc
    63481 atcagcaacg atgtggcccc agccatcttt atagctcatt aattttgcaa attccattcc
    63541 tacttcttct tccatttggc ttgttgcagg agaagattca taggcaacgt tattaccatt
    63601 ccatagcata gcaaaattgt acgctagtaa agatggcatc aatgtttcag agttcatatg
    63661 accccagtaa cgtccggcat tatgccaagg aacggaatgg gtacgcatcc gcgctgaaat
    63721 ttctgataga acatcctttg ttttattcac agtgtgttca aaactagcac tgcttttttc
    63781 ttctggcgta ataataggca tgtcttgagg catgtagttt tgacgccagc ctaaatgttc
    63841 atccaccaat tcattcaaca aagctttata gatttggccg ttctcagctt tgtccccaat
    63901 gaatagggca tttaggttta gatctttcga caatgattca ctcatagtga aaccctccta
    63961 aaaaaatatt taaattttgt cgagccctac attacacctg ttcttctatt ctgtaaacta
    64021 agttttggcc tgatttttaa tcatatttta atatgtatca attatccgaa ttgttcacac
    64081 ttttgaaata attaaaaaaa acgagggaat ttacaaatgt aaactccctc gcaaagattg
    64141 tattaacgac aaccagcgtt ctattttagg atgggtttca aaattggaat gttgtaatag
    64201 tatttccctt tttcaaggta aaaacttact aaattctaat caattacttt ggccaaaaag
    64261 taatttttct taccttttcg aataaccaca aacttaccat cgaattcatc ggatggattt
    64321 atgacaaaat ttaaatcggt aacgcggtca ccgttaatac ttatagctcc gtttgaaaca
    64381 tcttcacgtg cttgacgttt agatggttca atttttgtgg aaacaagcag ttccacgata
    64441 ttttcaggtg tgcttgaaat ttcaacgttc ggcattttac cgaatccttg cgcgatttct
    64501 tccgcattta gttctttaat atttccagag aacaatgctt gcgttatttt ttgcgcttct
    64561 ttcaagtctt cttcactatg cacaaatctt gtaacttctt ctgccaaacg gcgttgtgct
    64621 tcacgtttct caggttctgt ctctactttt ttcgctaagt cttcgatttc ttcttgagac
    64681 aagaaagtaa agaatttcaa gtatttcact acatcgcgat catcttgatt caaccagaat
    64741 tggtagaact caaatggtga agttttcttc ggatcaagcc aaacggcacc gcctgcagtt
    64801 tttccgaatt tcgttccgtc agccttcagc atcaatggga tcgttaaacc gaacgcttct
    64861 gcttctggtc cttctaattt tcggattagg tctaatccag ctgtgatatt tccccattgg
    64921 tctgctccac cgatttgcaa ttgcacatcg tatgttttat gcagatgcaa aaagtcgata
    64981 gattgcagga tttggtaagt aaactctgta aatgagattc ctacttccaa acggctggca
    65041 acgatatctt ttgctaacat cgtgttgatg ttgaagtttt taccgtagtc tctcaaaaat
    65101 tcaagtaaag agatttttga caaccagtcg tagttgttca caaatgtgat atttgcatct
    65161 ttaccaaaca attttctcat ttgattggaa agtgcgtcca cgttatgttg cacttgttcc
    65221 atcgtttgca acacacgttc tgttttgcgg ccgcttgggt cgccgatcgt tcctgttccg
    65281 ccgccaatca gaatatatgg atgatggcct gccaattgga atcttttcat catcataaat
    65341 ggaatcaaat gtccgatatg catcgaatca ccagttggat caacgccgca ataaagtgaa
    65401 atgctctttt cttcaacgag ctcgcgaagt ctttcttcgt ttgtttgctg attaatggcg
    65461 tcgcgccaag ttaattcatc aataatattc attttactga cctccgtgta gtttttttat
    65521 gaaagcagga acctgctagc atatgtaatt aattatttct tcaggatagg ataggaaata
    65581 aaaaaagtcc cttggcatat aagccaaggg acgaaatctc gcggtaccac ccaatttgta
    65641 tgtctagata tccaacatac ctctcgaaca tttaacgctg tcgcgtggca atcactgcca
    65701 aagactcctg aaatgtaatt cgctttaaag catgtactgg tttgcagcga ccaccagctt
    65761 tctgaaacat tggctttaac aactacttcg tttcgatctt cgtcttgtta ttattaatat
    65821 gtgtctatca tagagcgcaa ctttttcttt gtcaacagaa aaaggatatt ttttttaaat
    65881 tcctcatttt ttcttttttg aatccgctct aattccgaac attttatttt caattattgt
    65941 taaaagaaat aactttttct gtattttttc tgcaatttat cgagaagtca ctttactttg
    66001 cctcttattc tttcttcgtt acaataagga atgggacaag taaaaaaatg aaaaagcaag
    66061 ggacgaggta tatggaaaaa aagcaaaaaa ttattttagg agccacttct gctttaacta
    66121 tcgttatttt aattctaagt ttgttttttg tcatccgatc atctacaaaa aaagaaccaa
    66181 tcattcctga aaaaaaaata acagaaaaag caaaagaacc gacagatgat aagcaacaac
    66241 tagttactca agcaaaaaat ctggctcaaa gctacgatta tgaccaagcg attgcgttat
    66301 tggaaaaaga tagtaagaac ggatctactg aaacacaaca gcttcttcaa acactaaaaa
    66361 aggagaaaga acaactgatc gactggtctg atcctactca gatctctcat gtctttttcc
    66421 acagcttgat cgtggacccg caaaaagcct tccactctca acaggcacaa ggatacaaag
    66481 attatatggt gactgttgaa gaattcaacc gttcgatcga acaactttat caaaatgatt
    66541 ttgttttagt taatttgaat gatttgatcc aaaaagacga acaaggaaat ctttccttta
    66601 acgggcttgc gctacctgaa ggaaaaaaac cattggttct ttcacaagat gatgtcagct
    66661 attatgaata tatggataat tccggttttc ctagccgatt ggtcgtatca aataacaatg
    66721 aaatcaaaaa tgtctataaa gagaaagata aagaagaaat cggtgacttt gacatggttc
    66781 ctctgatcga tgcctttgtc aagaaacatc ctgatttttc ttatcgaggc gcaaaaggta
    66841 ccttggccct gacaggttac aacggagttt taggttatcg aacatcaaaa tctcaatatg
    66901 gagacaatga aaaaacaaaa aaagagatta aagaagctaa aaaagtggcg gaacagttga
    66961 agaaagacgg ctggagcttt gcttcccata catggggtca cttgaatatg actcaagctt
    67021 cactagaaga tgtaaaaaaa gacaatgaat tgtggcagca agaagttgca cctatcctag
    67081 gaaaaacaaa tattctgatt tatccatttg gtgcagacat cagcgactgg cagccttatt
    67141 cagcagaaaa tcaaaaattt gcttacttga agagtcaagg tttcgatatt ttctgtaatg
    67201 tcgatgcttc tacaccagct tggggacaat taggtacgga tttctatcga aatgctcgga
    67261 tcaatatcga tggcattcgt tttcaatctg atttagacgg aaaaaatcct attcttgatc
    67321 cattcatcaa tgtccatgat gtctatgaca aagaagcacg cgaatctgct tgattttacg
    67381 tcatttatga accaaaaaag ccgaactgac attttatcgt cagtttcggc ttttttatgc
    67441 tttatttctt cttcggtgcg attcccttga tcaggacttc tgtccattct gctgtatatg
    67501 gaagcagttt tgcgtatatc tccggcttag aaattccttc taccattgct tgaaaataga
    67561 ggatcaagaa ctcatccgaa tatttcaaat ccacttttcc ttctttccgc cctcgatgga
    67621 aaagatcaag catgatactg aaactttctt gcgtatacgc atccatcgcg tcagaaaatt
    67681 cttttttttc tttttttgta tagtatgtca tcaaatcgat ataaaattgt ttgttcactc
    67741 gattgagata acggattttg ttttcactca tggcaatcag tgtttcttca aatggaagat
    67801 tttccgcaat caacgctttc gcttcttgcc ctaaatcatt cataaatgca aaaaatactt
    67861 cataaatcag cccttccttg ctgccaaaat atttaaaaat cgtcgcttta cttacctgag
    67921 cgttctcagc taccttatcc attgtcactc cagcggttcc tatgtctgtg ttcatcaagt
    67981 caaaagctgc ttgtaaaatc tctttctttt tctcttgtgc tctcttttca aaaccgttca
    68041 tttgtagagc ctcccgtaat tttaaacttt tataatatat atagttcaaa tttattctga
    68101 ggtttattat aactatctat tgagaaagt
//
LOCUS       DAZKLM010000004        61713 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3764
            SAMEA115706913-rid22582563.denovo.004, whole genome shotgun
            sequence.
ACCESSION   DAZKLM010000004 DAZKLM010000000
VERSION     DAZKLM010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706913
            Sequence Read Archive: ERR13196647
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 61713)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 61713)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287823.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287823.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,004
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,849
            CDSs (with protein)               :: 2,849
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 41 of 96
            Pseudo Genes (incomplete)         :: 52 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 15 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..61713
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706913-rid22582563.denovo.004"
                     /isolate="VRE3764"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(132..509)
                     /locus_tag="ACG842_000204"
     CDS             complement(132..509)
                     /locus_tag="ACG842_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0246775.1"
                     /translation="MEDAIKQGQLFLSELTAEERSCLYNDLLSIKDVYLCNKDTSFYT
                     FRKIRRATKRCAECRKRTFLYQTEHKGELSISQIHEQKRFKNHYRIQILFNGQEMEYA
                     FLENLLQHLHNQHFSYNSQAIYA"
     gene            765..1493
                     /gene="purC"
                     /locus_tag="ACG842_000205"
     CDS             765..1493
                     /gene="purC"
                     /locus_tag="ACG842_000205"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HFQ0246776.1"
                     /translation="MEKKELLYEGKAKKIYLTSDENLIWIEYLDQATALNGARKDQIS
                     GKGELNNQITSAIFRYLTQRGIKNHFIEQLSKTEQLAKKVQIIPLEVVIRNIAAGSFS
                     KRLGVPEGKEFIKPIIEFYYKDDQLDDPFINEEHIYFLQLAKPEEISELKAQALAINE
                     ILTELFAKVGLVLVDFKLEFGRSNGQILLADEVTPDTCRLWDERTNEHMDKDVYRRDL
                     GDLIPVYQEVYNRLSELTEEKERV"
     gene            1493..1747
                     /gene="purS"
                     /locus_tag="ACG842_000206"
     CDS             1493..1747
                     /gene="purS"
                     /locus_tag="ACG842_000206"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HFQ0246777.1"
                     /translation="MYFVKVYVTYKDSVLDPQGEAVKKAVHRMGYETIEDIRIGKYFE
                     IQLADAEEPIDTVIETICDKLLANVNMETYRYEIKKMEEV"
     gene            1749..2420
                     /gene="purQ"
                     /locus_tag="ACG842_000207"
     CDS             1749..2420
                     /gene="purQ"
                     /locus_tag="ACG842_000207"
                     /EC_number="3.5.1.2"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HFQ0246778.1"
                     /translation="MKFAVIVFPGSNCDADMLWAVREIMGADAEFVRHDESSLEGFDG
                     VLLPGGFSYGDYLRCGAIARFSTIMEEVIRFAEEGKPVFGTCNGFQILTEAGLLPGVL
                     RRNESLHFICKTVPLKVNPKTAFTSEYEADEIIYLPVAHGEGNYFCDEETLQELKKNN
                     QIAFTYNDNINGSVENIAGITNKAGNVLGMMPHPERAVEDLLGSEDGKRFFASILKNF
                     GKVTV"
     gene            2421..4643
                     /gene="purL"
                     /locus_tag="ACG842_000208"
     CDS             2421..4643
                     /gene="purL"
                     /locus_tag="ACG842_000208"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HFQ0246779.1"
                     /translation="MKTLKEPTAEEIKKKRIYAEWGLTDEEYRMIEEDILGRMPNYTE
                     TGLFSVMWSEHCSYKNSKPVLRKFPTSGPHVLQGPGEGAGIVDIGDGLAVVFKAESHN
                     HPSAVEPYEGAATGVGGIIRDIFSMGARPIALLDSLRFGELDNPRTKYLLEEVVAGIG
                     GYGNCIGIPTVGGEIAFDPCYEGNPLVNAMCVGLIEHKHIQKGQAAGVGNSIMYVGAK
                     TGRDGIHGATFASEEFNQEEEQQRSAVQVGDPFMEKLLLEACLELILHHSDSLIGIQD
                     MGAAGLVSSSAEMASKAGSGLILTLDEVPQRETGMTPYEMMLSESQERMLICVKSGEE
                     EKIQELFQKYDLDAVTIGKVTDDGQYRLYHQGEEVANLPVDALAEDAPVYHKEMKEPA
                     RIVEFQQLAPYQPVIEEPGQVLLDLLQQPTIASKRSIYETYDSQVQTNTVVRPGSDAA
                     VMRVRGTNKALAMTTDCNARYLYLDPKIGGQIAVAEAARNIIASGGKPLAITDCLNYG
                     SPDKPEVFWELSTSADGIAAACETLGTPVISGNVSLYNETDGQAIYPTPMIGMVGLIE
                     DHKHITTQEFKKSGDLIYILGKTFADFDGSELQKMQLGRIEGVIRNFDLSIEKRNQEL
                     VLTAIQNGLIESAHDCSEGGLAIALAESAFKHQLGISVQFELSSAQLFAETQSRFVLT
                     VAPENKTRFEEMMGDAAVLAGKVTDEAIIEISATDGQIKIETAVARKCWEDAIVCLMK
                     "
     gene            4628..6067
                     /gene="purF"
                     /locus_tag="ACG842_000209"
     CDS             4628..6067
                     /gene="purF"
                     /locus_tag="ACG842_000209"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HFQ0246780.1"
                     /translation="MSYEVKSLNEECGIFGVWGHPDAASVAYFGLHSLQHRGQEGAGI
                     VSNDRGKLKGHRDLGLLSEVFKDQRKLQQLTGEAAIGHVRYATAGNGSVDNIQPFLFK
                     FHDQSIGLAHNGNLINARSLRKELEENGAIFHSNSDTEILMHLIRRSEETDFLNKLKS
                     ALNEVKGGFAYLLMTETAMIAALDPNGFRPLAIGQMKNGAYVIASETCALEVIGARFI
                     RDVQPGEIVIINDEGIQIDCFTKDTQLSICSMEYIYFARPDSNIAGINVHTARKNMGR
                     NLAKESPVKADMVVGVPNSSLSAASGYAEASGIPYEIGLVKNQYIARTFIQPTPELRE
                     QGVRMKLSAVRGVVEGKKVIMVDDSIVRGTTSRRIVHLLKEAGAKEVHVRIASPPLKY
                     PCFYGIDIQTRKELIAANHTIDEIKECIGADSLGFLSEEGLIESIGLEKDAPYSGLCM
                     AYFNGDYPTPLYDYEEKYQESLKEKVSFF"
     gene            6090..7142
                     /gene="purM"
                     /locus_tag="ACG842_000210"
     CDS             6090..7142
                     /gene="purM"
                     /locus_tag="ACG842_000210"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HFQ0246781.1"
                     /translation="MNSMANAYAKAGVDVEAGYEVVERIKKHVKKTERLGMMGAIGGF
                     GGCFDLSVFQLKEPVLVSGTDGVGTKLAVAIEADKHDTIGIDCVAMCVNDILAQGAEP
                     LYFLDYLATGKNDPLRLENVVKGVADGCVQGNMALIGGETAEMPGMYSGNDYDLAGFA
                     VGIAEKSQLLTGETINPGDVLLGIASSGIHSNGYSLVRKIFFEQQQFSLSSKLPELKK
                     TLGEELLEPTKIYVSSLLPLIKEKKIKGAAHITGGGFVENIPRMLPESMAAQIKLGSW
                     PILPIFGCLQKYGEIPEMEMYEIFNMGIGMVLAVQESKVKEIVHEIEQAGEKVYEIGK
                     VIAKEKHRVVFIGEER"
     gene            7139..7717
                     /gene="purN"
                     /locus_tag="ACG842_000211"
     CDS             7139..7717
                     /gene="purN"
                     /locus_tag="ACG842_000211"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HFQ0246782.1"
                     /translation="MRIAVFASGNGSNFQALADYLSKKGLESSIDWLFCDQPEAYVLK
                     RATALSVPADCFSPKEFDSKKEYEEAILHKLKEKKIDLIVLAGYMRIIGPVLLKNYDK
                     RIINIHPSLLPAFPGLHGIRDAFEAGVKETGVTIHYIDQGVDTGPIIRQEKVRIEQED
                     TFDSLEEKIHRVEHRIYPEVISEIIENGGYLK"
     gene            7714..9249
                     /gene="purH"
                     /locus_tag="ACG842_000212"
     CDS             7714..9249
                     /gene="purH"
                     /locus_tag="ACG842_000212"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HFQ0246783.1"
                     /translation="MTRALISVSDKTGIVAFAQGLIEQGVEIISTGGTKHALDKAGIK
                     TLSIEEVTGFPEMMDGRVKTLHPKIHGGLLGRRDLPSHMEAMETENIQPIDFVCVNLY
                     PFKETIMKPDVEIAEAIENIDIGGPSMLRSAAKNHEFVTSVVDPSDYEEVLSELRQYG
                     HTSLSFRQKLAAKVFRHTAAYDALIAQYLTDWVEEKEPEKLTLTYERKQTLRYGENSH
                     QAAAFYQSALLEKYSIAAAKQLHGKELSYNNIRDADAALRIMKEYDEPTVVALKHMNP
                     CGIGTASTILEAWEAAYEADPVSIFGGIIVLNREVDLPTAEQMHKLFLEIIIAPSYTQ
                     EALELLQKKKNIRLLTVDFAQEENQNREETVSVLGGLLVQDQDLAIENEEEWKVVTKR
                     KPTPAEAKALAFAWRAVKHVKSNAIVLANERQTVGIGAGQMNRVGSVKIAIEQAQEKM
                     EGAVLASDAYFPMDDSVEYAAKHGIKAIIQPGGSIKDQDSIDMANKYDLAMVFTDVRH
                     FRH"
     gene            9265..10539
                     /gene="purD"
                     /locus_tag="ACG842_000213"
     CDS             9265..10539
                     /gene="purD"
                     /locus_tag="ACG842_000213"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HFQ0246784.1"
                     /translation="MNILVIGAGGREHAISKKLLASPKVDTVYCAPGNPGMTKDGINC
                     VAISERDHSALIHFVKKEAIDWSIVGPEVPLLNGIVDDFMAEGLKIFGPTKAAALIEG
                     SKSFAKQLMDTYQIPTAKSRSFECYEKAAAYIKENGAPIVVKADGLAAGKGVVVATTV
                     KEALDAAEQMLNQHRFGDSSKKIVVEEFLAGEEFSLLAFVRNTKVYPMVISQDHKRAF
                     DNDKGPNTGGMGAYSPVPQISKKMIEKAVEEVLKPAALGMKELGRSFTGVLYAGLIAT
                     KEGPKVIEFNARFGDPETQVVLSRLKSDFAQVIDDLLENRTVELKWQQEGYNVGVVVA
                     AAGYPEEYETGMVLPDFLEEELSVYYAGVSSNNGKLVGAGGRLYLVEAYGETIKEAQK
                     KIYTALMDKTTDGTFYRTDIGEKAANSELIVK"
     gene            10661..11575
                     /locus_tag="ACG842_000214"
     CDS             10661..11575
                     /locus_tag="ACG842_000214"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HFQ0246785.1"
                     /translation="MKKIQLGTSDQKAAAVILGCMRINNAEDPVKVIETAYENGIDFF
                     DHADIYGNGECEEIFADALAKTSIKREDLFIQTKCGIVPGVMFDFSKKHIIESVEKSL
                     TRLKMDYVDALLLHRPDTLVEPEEVAEAFDELERSGKVRYFGVSNQKPMQIELLKKYV
                     RQPLVANQLQFGLKHTGMIDQGIHVNMTDEASIDHDGSVLDYSRLHDMTIQAWSPYQY
                     GFFEGVFIGNEKFPELNQTLEELAAKYQTTPTGLASAWILRHPANMQVIAGTMNVGRI
                     KEIAQASEINMSREDWYELYRAAGNILP"
     gene            complement(11708..13273)
                     /locus_tag="ACG842_000215"
     CDS             complement(11708..13273)
                     /locus_tag="ACG842_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0246786.1"
                     /translation="MYKVFIIEDEYLIRNNLREQITVLGSYYPITYVGEAGDGEMALS
                     SIIDLQPDIILTDIQMPFMDGLTFSKEARKLLPWTRLIFISGFDDFDYMKGAIQVQAD
                     DYLLKPIKSSELKQAIEKAVTALDLQQKETHTSSDLVFELKKNHFLNGLFKGSLTISE
                     TLKLAESFNRSLAGKKMTVLLAANQVSTDFEDYAHLSDYLKFLFRENQQVIFSAVSSR
                     FIKFLIVDSLEENVIETSYQVAQTLVHELEKGQKNNFSVAIGPVAERISELPASFEIT
                     QMILETASSLKEQIISYEDLIQEAKLPVDSVFYFDLSLDLSSLSKNEVSKYIEHLTKP
                     QGTPTRTRLYRLFVLTKLLQHVCLKEKKIPQEMVHCTSTSQRLAISADDSLYRSTVYM
                     FISFLTALPSQPSRNKYQPILNHALDFIKENFTDPDMSLTWVAQQVALSPSHFSTIFS
                     QSFNQTFIDYLTEQRIELAKKLLQQSDYRISDIAFEIGYNDPNYFSYLFKKKQGISPK
                     EYRTQLFLKNKNN"
     gene            complement(13266..14717)
                     /locus_tag="ACG842_000216"
     CDS             complement(13266..14717)
                     /locus_tag="ACG842_000216"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFQ0246787.1"
                     /translation="MTKNLSIKQIFAQANKAMLLFAVIPLLLSILLYSQQLLIYQQTI
                     ANIQKADAIALKVDQKVLESMWDLISGQVTVDEFNEENILEELRNDIREIKNNTNTLK
                     EISTLDVSLRTIDTLEDYQNELINNILAKDFMDTNEEIINQVDSVTQLLSDILQDFVR
                     VEIDLASKKNTTLVRSLILLTIVELGVLSTIFFYAKKNRRFLERKVQEPINQLIEMSE
                     QLSSGNLGFRLTLPETLELQTLTASMNKMAEDLNQSLRENALKQYHLAQSEVRVLQAQ
                     ITPHFIYNSLDAIISLIEQDCFEEALTMTHALSGFFRTSLSKGQDWIPLEKEISHIND
                     YLVILKIRYGHMLDYKIDIPETLFPVSILKMILQPLIENAVYHGTKYVRHKGFIRITT
                     CKIDEMIQFKVIDNGIGIPAEKLAEIRKELAKGIDSDFSIGYGLYNVNKRLLLYYGND
                     QKPITIDSKYREGTTVTLMIPLSPKTIGGNKHV"
     gene            complement(14731..15747)
                     /locus_tag="ACG842_000217"
     CDS             complement(14731..15747)
                     /locus_tag="ACG842_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFQ0246788.1"
                     /translation="MKRVLFFLALVVIAYQMMGCTPNTKEAPDPLVVGFSQSGTESSW
                     RKKHTESIITALEKEDYQVLYRNGYMNQERQIQDIRTFIAYKVDMIIFTPLQESGWDS
                     VLKEAKRAGIPVILVDRHIQTNDPELFVTHIGPSFKAEGNRAGLFVSNHFTNSKKQEI
                     RILELAGSENSTPTKLRSEGFLESINRKPTLKKDGTIDHKPPMTIVHRIVGDFIRMKG
                     KDQMKRYLETNDLSEIDVLYSHSDEMTHGALAAIKETSIKPGEDLIIVTIDGQEDMIE
                     KLRSGIVNCVVECKPDVGWYVANTVTRYFGNIQTGKTLPKDIFISETVFSQQNIANIP
                     TRNY"
     gene            15967..17055
                     /gene="chvE"
                     /locus_tag="ACG842_000218"
     CDS             15967..17055
                     /gene="chvE"
                     /locus_tag="ACG842_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multiple monosaccharide ABC transporter
                     substrate-binding protein"
                     /protein_id="HFQ0246789.1"
                     /translation="MKNKFRKIVGTIAILTASILLSACGNSGSADDSTGYVGIAMPTK
                     SAERWIADGNNMVSELEKLGYKTDLQYGEDKVENQVAQIENMITKGVDTLVIASIDGS
                     ALTDVLAKAKEADIKVIAYDRLLMNSENVDYYATFDNFGVGVSQAAYIEEHLGLKEGK
                     GPFTIELFGGSPDDNNALINYNGVMSVLQPYMDNGQLVVPSGQTSFSQIATLRWDGST
                     AQARMDNLLSANYTDQTLDAVLSPYDPISLGIISSLKGVGYGSESKPLPVITGQDATV
                     AGVKSIIAGEQTQTIFKDTRILAKNTIEMIKAISDGEEVPVNDTETYDNGVKTVPTYL
                     ANTVSVDKDNYQAELIDTDYYKESDLKN"
     gene            17109..18635
                     /gene="mmsA"
                     /locus_tag="ACG842_000219"
     CDS             17109..18635
                     /gene="mmsA"
                     /locus_tag="ACG842_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multiple monosaccharide ABC transporter
                     ATP-binding protein"
                     /protein_id="HFQ0246790.1"
                     /translation="MADCILKMADITKKFSGIRALDCVNLMIERGEIHALCGENGAGK
                     STLMNVLSGLYPYGSYDGEIFYDGKLCQFKDLKDSEEKGIVIIHQELALSPYLSIKEN
                     IFLGNEQAERGVIDWELTEKKTYELLNKVGLSIDPNLLVSQIGVGQQQLVEIAKALSK
                     SVRLLILDEPTAALNEEESANLLRLIRELKEQGVTSIIISHKLNEIVDIADRITILRD
                     GQTIETLENEMISEEHIIRGMVGRDLVDRYPERKPDIGEVYFKVEDWTVYHPLDHERI
                     INDHINLHLKKGEIVGIAGLMGAGRTEFAMSLFGHSYGSKISGRILKEGAEISVKTVP
                     SAIANGLAYVSEDRKSLGLNLLMDIRENVTLSSLDKISQKGVLDKEKEVTEAENFRKK
                     MRIKTNSIHQIVSSLSGGNQQKVVLSKWLMTQPDILFLDEPTRGIDVGAKYEIYTIIE
                     EMAANGKAVCLISSELPEILGMCDRIYTMNEGRITGEVSREDADQEVLMKLMTKEEVG
                     "
     gene            18637..19842
                     /gene="mmsB"
                     /locus_tag="ACG842_000220"
     CDS             18637..19842
                     /gene="mmsB"
                     /locus_tag="ACG842_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multiple monosaccharide ABC transporter
                     permease"
                     /protein_id="HFQ0246791.1"
                     /translation="MEQAKNLTDKKEKKLNLKSRAADLFGRYSMVIILAGLLIAFQLM
                     TDGIFWRPLNITNLVLQNSHILVLAAGMLLVVLLGHVDLSVGSVMAFVGAIAGMMMVN
                     RQMNPWMTVIACLAVGGLIGAWQGFWVAYAGIPAFIVTLAGLLMFRGLTQVVLGGQSL
                     APFPAVFQKMSTGYLPDLSEGSSLHFLTLILGMIITIVLVVGQWRTRNRRKNHLFEVE
                     SPTAFLVKAVFTAVVVLGITYIFASYQGYPIILIILGLIVAAYGFLTNKTVAGRQIYA
                     TGGNRKAAELSGIKTKRITFWVFVNMGVMAALAGLVLAARLNAATPQAGTSMELDAMA
                     AVYFGGASTSGGIGTIMGAIVGGLVMGVLNNGMSILGVGVDWQQAIKGLILLLAVVLD
                     LYNKKKRTT"
     gene            20099..21076
                     /gene="guaC"
                     /locus_tag="ACG842_000221"
     CDS             20099..21076
                     /gene="guaC"
                     /locus_tag="ACG842_000221"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902560 - GMP reductase complex [Evidence
                     IEA];
                     GO_function: GO:0003920 - GMP reductase activity [Evidence
                     IEA];
                     GO_process: GO:0006163 - purine nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HFQ0246792.1"
                     /translation="MKVFDYEDIQLIPNKCIVNSRSECDTTVTLGKHTFKMPVVPANM
                     QTIIDETIAEFLAENGYFYIMHRFDEAARIPFIKKMKKRGLITSISVGVKKEEYSFIE
                     KLAEESLNPDYITIDIAHGHANSVIDMIQHIKKYLPETFVIAGNVGTPEAVRELENAG
                     ADATKVGIGPGKVCITKIKTGFGTGGWQLAALRWCAKAARKPIIADGGIRTHGDIAKS
                     VRFGATMVMIGSLFAGHEESPGETKVENGIVYKEYFGSASEFQKGEKRNVEGKKIWIQ
                     HKGSLKDTLVEMQQDLQSSISYAGGRDLEAIRKVDYVIVKNSIFNGDTI"
     gene            21340..22764
                     /locus_tag="ACG842_000222"
     CDS             21340..22764
                     /locus_tag="ACG842_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HFQ0246793.1"
                     /translation="MWEKVEKKQGPIYRQIMEQIMREIENGRINPGSQLESERKLGQL
                     FGVNRSTVVHALEELRELGVLTSKRGSGWFVNQTEWGKFAVPRIDWRQMISPRYEQTD
                     WYEQKIREAKQIQKTSFLDLYASEMPDELLPNFEFPSYSLEEILAEEKEMDLLGYTPL
                     RNKIKRYLESKFELAFSPNQLLVTGGGQQAIFLILQTILSAGEGIAVESPSFFYRLPL
                     FKASGTRLFGIPMDEEGIDLEKLKESIHKNKLKAVLINPNFQNPTGKVMSQKRREKLV
                     ELCRQYRLPIIEDDVFTDLSFLNQEEVLPIRTIDPDNVLYVGSLSRVLGKTTKIGWVI
                     GPETFISQLAQAQEMMEFSMNILTQITVANVFNEAIDLKMQDVRKKLKEKSRLISAWG
                     DSQSFFKLHSILGGYYAWITWDGKKLDPELAEKVVDMGLGIAPSLLFGQDINGIRINY
                     SRLDENQIPLFYSKMKILEKWLTE"
     gene            complement(22806..23063)
                     /locus_tag="ACG842_000223"
     CDS             complement(22806..23063)
                     /locus_tag="ACG842_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0246794.1"
                     /translation="MPIRILTALVNKITIRKAFYISSAFFLFSNQTYNHLFLFKNVGV
                     IQFCCKHLFYLHIVNGRTNNGFFLVSLYRILFPKKKKQLEI"
     gene            23225..24334
                     /gene="pdhA"
                     /locus_tag="ACG842_000224"
     CDS             23225..24334
                     /gene="pdhA"
                     /locus_tag="ACG842_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HFQ0246795.1"
                     /translation="MANKKAPIDFQALLDEVNADFPVYQALDQDGNVVNKDLVPDLSD
                     DELVELMTHMVWSRVLDQRSTALNRQGRLGFFAPTAGQEASQLASAYAFDKEDVLLPG
                     YRDVPQLVKHGLPLSQAFLWSRGHAAGNSYPEELKALPPQIIIGAQYVQAAGVALGLK
                     KRNKKNVVFTYTGDGGSSQGDFYEAINFAGAYHANAVFYIQNNGYAISTPREKQTAAK
                     TLAQKAVAAGIPGIQVDGMDPLAVYTVSKMAREWAISGNGPVLIETLTYRYGPHTLSG
                     DDPTRYRTKDTDDDWQKKDPLIRFRKYLTEKGLWSEEKEEQVIEATKEEIKAAIAEAD
                     KVPKQKVSDFLKNMFEVSPQSIKEQIAIYEAKESK"
     gene            24337..25314
                     /locus_tag="ACG842_000225"
     CDS             24337..25314
                     /locus_tag="ACG842_000225"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HFQ0246796.1"
                     /translation="MAQKTMIQAITDALALELENDENVVVFGEDVGKNGGVFRATEGL
                     QEKFGEDRVFDTPLAESGIAGLSFGLALEGFRPVPEIQFFGFIFEAMDEVVAQMARTR
                     YRMSGTRNLPITIRSPFGGGVHTPELHSDNLEGLIAQSPGIRVVIPSNPYDAKGLLIS
                     SIRSNDPVVFLEHMKLYRSFREEVPDEAYEVPLDKAAVTREGTDVSIITYGAMVREAI
                     KAADNLAKENISVEIIDLRTVAPLDVETIIQSVEKTGRVVVVQEAQRQAGVAAQVVSE
                     ISERAILSLEAPIGRVSAPDTVFPFGQAENVWLPNAKDIEDKVKEIAEF"
     gene            25339..26982
                     /locus_tag="ACG842_000226"
     CDS             25339..26982
                     /locus_tag="ACG842_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HFQ0246797.1"
                     /translation="MAYQFKLPDIGEGIAEGEIVKWFVKPGDTINEDDTLLEVQNDKS
                     VEEIPSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHEDNEGDSGVAAESQTPAKPAA
                     EPTVDTESAGSSSEGVFQFKLPDIGEGIAEGEIVKWFVKPGDTINEDDTLLEVQNDKS
                     VEEIPSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHNSAPSTSAPSAEAPKEKVETS
                     GSASVVEAADPNKRVLAMPSVRQFAREKDVDISQVTATGKGGRVTKEDIENFLAGGPS
                     SAPAKSEAPEAAAPKEAAPAAESKPAAPAKPFKSNLGDLEERVAMTPTRKAIAKAMVN
                     SKHTAPHVTLHDEVEVSKLWDNRKRFKEVAAANGTKLTFLPYVVKALTATVKKYPVLN
                     ASIDDANQEIVYKHYYNIGIATDTDHGLYVPNVKDADRKGMFAIADEINEKAKLAHDG
                     KLSAEDMRNGTITISNIGSVGGGWFTPVINYPEVAILGVGTIAQQPIVNAEGEIVVGR
                     VMKLSLSFDHRIVDGATAQQAMNNIKRLLADPELLMMEG"
     gene            26990..28396
                     /gene="lpdA"
                     /locus_tag="ACG842_000227"
     CDS             26990..28396
                     /gene="lpdA"
                     /locus_tag="ACG842_000227"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HFQ0246798.1"
                     /translation="MVVGDFAVELDTVVIGAGPGGYVAAIRAAEMGQKVAIIEREYIG
                     GVCLNVGCIPSKALIAAGHHYQESLDSEMFGVTSENVKLDFAKTQEWKENKVVHTLTS
                     GVGFLLKKHKVETIEGEAFFVDDHTLRVIHPDSAQTYSFNNAIIATGSRPIEIPGFKF
                     GGRVLDSTGGLALKEVPKKFVIIGGGVIGAELGGAYANLGAEVTILEGSPQILPTYEK
                     DLVKLVEDDFKKKGVTVVTNAMAKEAVDNGDSVTVKYAVDGKEESVTADYVMVTVGRR
                     PNTDDMGLEQAGVEVGERGLITVDKQGRTNVPNIYAIGDIVPGAALAHKASYEAKIAA
                     EAISGKKVAVDYKAMPAVAFTDPELASVGMTIKEAKDAGIEAKAYKFPFSGNGRALSL
                     GKTEGFIRLVTTIEDNVLIGAQIGGVGASDMVSELALAIESGMNAEDIALTIHPHPSL
                     GEIVMDASELALGLPIHI"
     gene            28558..28833
                     /locus_tag="ACG842_000228"
     CDS             28558..28833
                     /locus_tag="ACG842_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HFQ0246799.1"
                     /translation="MQEYQYPLDLDWTTDEMVIVMKMWEDLERANEKGINNEAFLKTY
                     QQFKTVVKSIGEEKRLGREFEKASGYSLYRTVQQAKKNPNKTLRMNG"
     gene            28839..29606
                     /locus_tag="ACG842_000229"
     CDS             28839..29606
                     /locus_tag="ACG842_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HFQ0246800.1"
                     /translation="MEQMIDHIKSWLEEAGEKIKSSQQTLTISQKSNRTDLVTNVDRN
                     IQNFLIEKIHSFDPEAKILAEEEGCNQLSDLDGRVFIIDPIDGTLNFVIEGENFCIML
                     AVYESGVGKLGFIYDVMRNELFWGGKGIGVYKNDEPLPRPANKSLSEGLLGVNSYLFG
                     HNKYGIREIAESSMGTRMYGCAGLELVAILKGNHIAYISNLSPWDYAAGNVLLEEFGM
                     VTSGLFGTPLTFEKREYYLAGTSCAYAEIADLLKKEG"
     gene            29774..31603
                     /gene="typA"
                     /locus_tag="ACG842_000230"
     CDS             29774..31603
                     /gene="typA"
                     /locus_tag="ACG842_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HFQ0246801.1"
                     /translation="MNYRNDIRNVAIIAHVDHGKTTLVDELLKQSDTLDAHTQLQERA
                     MDSNALEKERGITILAKNTAVDYKGIRVNIMDTPGHADFGGEVERIMKMVDGVVLVVD
                     AYEGTMPQTRFVLKKALEQHITPIVVVNKIDKPSARPEHVVDEVLELFIELGADDDQL
                     DFPVIYASALNGTSSLSDDPADQEPTMAPIFDTIIEKIPAPVDNSDEPLQFQVSLLDY
                     NDYVGRIGIGRVFRGTIKVGDQVALIKLDGTVKKFRVTKLFGFFGLKRLEIQEAKAGD
                     LIAVSGMEDIFVGETVTPVDHQDALPILHIDEPTLQMTFLVNNSPFAGREGKFVTARK
                     IEERLMAELQTDVSLRVEPTNSPDAWTVSGRGELHLSILIENMRREGYELQVSRPEVI
                     EKEIDGVKCEPFERVQIDTPEEYMGSVIESLSLRKGEMQDMVHTGNGQIRLTFLTPAR
                     GLIGYSTEFLSMTRGYGIMNHTFDQYLPMLPGQIGGRHQGALVSIDTGKATTYSIMSI
                     EERGTVFVEPGTEVYEGMIIGENSRDNDLTVNITKAKQMTNVRSATKDQTSVIKKPKQ
                     LTLEESLEFLNDDEYCEVTPESIRLRKQILEKNAREKASKKKK"
     gene            31865..33637
                     /gene="recQ"
                     /locus_tag="ACG842_000231"
     CDS             31865..33637
                     /gene="recQ"
                     /locus_tag="ACG842_000231"
                     /EC_number="5.6.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HFQ0246802.1"
                     /translation="MNDLLKQYFGYDEFRPGQKEIIQKVIDQENVLGIMPTGSGKSIC
                     YQLPALLLDGLTVVVSPLISLMKDQVDAANQLGIPATFINSSLDGYETARRFQEIDRQ
                     QYRLLYIAPERFIMPDFIQAMKRWNVRMIAIDEAHCISQWGHDFRPSYLQMANQLDQL
                     PNRPVIVALTATATVQVAADIKRLLKIPENNHIQTGFERENLRFQVIKDQKKEQYLIE
                     YLKINKNQSGIIYAATRKEVDRLYHLLKKFDFSVGRYHGGLNENERTEMQEAFLYDRL
                     QLIVATNAFGMGINKSNVRFVIHYQIPGSLEAYYQEAGRAGRDGLSSEAILLFSPQDI
                     QVQKFFIQQSQREEGQKQKEYEKLKAMTEYVYIESCLQQYILNYFGETSSPCNRCGNC
                     LDDREIVEVTTEAQMVLSCLKRMGENYGKQMLMKVLAGSKEQKLQALGFGHLSTYGLM
                     KNQSQKETMQLIEYLISNGYLLTINGEYPVLKVTERGIQVLKGQESVYRKEPKKVQQL
                     SDEETDTLFEVLRELRTDLASEAGVPPYVVFSDSTLKEMSRIRPSSRLEMLQIKGVGQ
                     SKLDKYGEAFLSRIKNADSNVLDK"
     gene            33751..34053
                     /locus_tag="ACG842_000232"
     CDS             33751..34053
                     /locus_tag="ACG842_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaN family protein"
                     /protein_id="HFQ0246803.1"
                     /translation="MDSLSKKLAIQVLNDEAERIKRLIRNQNNSLCISQCKAFEEVVD
                     TQMYGFSRQVGFAVRIGVIGKNEGQLMLSELERELNQLYSEVYQEIYDKKEVGKEE"
     gene            34056..35219
                     /locus_tag="ACG842_000233"
     CDS             34056..35219
                     /locus_tag="ACG842_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HFQ0246804.1"
                     /translation="MPKKVKKRHLLDYSILIPYLILCVLGLIMVYSSTSYLLLENGQN
                     PSASVINQSIFWVLSLIAIALLYKMKTDVLKNQRLIMAAIAVLTILLLIVVFFGKEIN
                     GAKGWLQIAGFSIQPAEYLKIISIWYLSLTLSKRQNSVQKDFLGTVKRPLAMVIGLTA
                     LVAILPDFGNAAVIFLIILVLLLASGVNYVYTLIVGVGGFCLSTFTIWLINITNGKIL
                     PGRLQYIYNRFAIYQNPFSDELNKGHQLVNGYYAMFNGGLFGRGLGNSIQKKGFLQEA
                     QTDFIYAIVVEELGVIMGILILALLFFMIVRIVLVGIRSKDPFNSLLCIGIGAMFLIQ
                     VFVNLGGITGVIPLTGITFPFLSQGGSSLLMLSICVGFVLNISADEKRKSLGL"
     gene            35245..38673
                     /locus_tag="ACG842_000234"
     CDS             35245..38673
                     /locus_tag="ACG842_000234"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004736 - pyruvate carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009374 - biotin binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HFQ0246805.1"
                     /translation="MKKVLVANRGEIAVRVFRACTELGIKTVGIYAEEDEYSVHRFKA
                     DEAYLVGQGKKPIDAYLDIEGIISIAKECGADAIHPGYGLLSENLNFAQRCEEEGIIF
                     VGPKLHHLDIFGDKIKAKAAAIEAGIASIPGTDGPIASIDDALEFAKQYGYPIMIKAA
                     LGGGGRGMRVAHDEKSAREGYERAKSEAKAAFGSDEVYVEKYIANPKHIEVQILGDTH
                     GNVIHLFERDCSVQRRHQKVVEVAPCVSMNEQQRQKICQAAVQLMKYVGYVNAGTVEF
                     LVEGDDFYFIEVNPRVQVEHTITEMITDIDIVTTQLLIAQGLDLHKEIGLPQQEGIKL
                     NGSAIQCRITTEDPLNNFLPDTGKIDTYRSPGGFGVRLDVGNAYAGYVVTPYFDSLLV
                     KVCTHGATFETAIQKMERCLKEFRIRGVKTNIPFMLNVITHPEFQSGNAKTTFIDSTA
                     TLFEFPRLRDRGNKTMKYIGEITVNGFPGIESGEKPFYEEPRMPKDLITRTDYVTAKN
                     VLDAKGADALVEWIKGQENLLLTDTTFRDAHQSLLATRVRTKDFKQIARLTGEGLPEL
                     FSSEMWGGATFDVAYRFLNEDPWQRLRKIRSLMPNTLLQMLFRGSNAVGYSNYPDNVL
                     VEFVKEAAAQGIDVFRIFDSLNWTPQMEKSIQAVRDTGKIAEAAICYTGDINDPSRAK
                     YNVQYYKDMAKELEQLGAHIIAIKDMAGLLKPQAAYRLISELKETTDLPIHLHTHDTS
                     GNGIITYSAASKAGVDIVDVAMSAMSGNTSQPSMSSLYYALVNGPRLPEITIENAQKL
                     NHYWEDVRMYYKPFENGLNAPETEVYMHEMPGGQYSNLQQQAKAVGLGHRWDEIKQMY
                     HTVNLMFGDIVKVTPSSKVVGDMALFMVQNDLTEEDIYEKGETLSFPESVVTFFQGEL
                     GQPVGGFPKKLQKIILKGRPALNERPGLLAESVDFNEVKKELAEKIGYEPKQDEVLSY
                     LMYPQVFLDYQKAYNQFGDVTLLDTPTFFQGIRLGETINVQIERGKTLIIRLDEIGEP
                     DIEGNRVLFFNLNGQRREITIKDNSIISTVQTRLKAEPTNREQIGATMSGSVLDVLVK
                     KGDNVKKGDTLMITEAMKMETAIEARFDGEVAHVYVSSGDTISSGDLLIEVTEK"
     gene            38694..39818
                     /locus_tag="ACG842_000235"
     CDS             38694..39818
                     /locus_tag="ACG842_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP-associated domain-containing protein"
                     /protein_id="HFQ0246806.1"
                     /translation="MKRLGLFWLILLLVIVAFYMEPVLFPPKLEVKENSDQQISNQMT
                     TWKYQELKATGFSTYIGKPIDQLEKDFGQAYDRLTSGFGFETRYYENKASHLTFEANI
                     QDSRVTTVKVLQTGPADIAPFYLGMTMQDLTKITTIYTNFTFEYHDTEVGIELMEEDM
                     NYRPLIAFDNDTFAILFFDQTTNGLFSVVYLDKDSLLKLLPYQVFGEGLPHYQADKSA
                     DWESINRVKERKSTTLLNMLRREDELPIYNQTLAFQNHSQLLLHDFLTRPEEILSEER
                     MADWQKSLGSAQTTVKFSLTNDELEKLISKQKLENTSGLFIHPAIDATFSFLYWYSDP
                     YNHDRFMDPGTDSVGIAFSKENMLVLLQEENGESSDSSDR"
     gene            39799..40245
                     /locus_tag="ACG842_000236"
     CDS             39799..40245
                     /locus_tag="ACG842_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HFQ0246807.1"
                     /translation="MIAVIVNEELFQLEDQCLQVAEMIKKSKTMASYLEHKQSMEESA
                     KVCQLKEAFLSKKEAFENVAAYGEYAPDYREKKRAVRKAKRQLDMDEHVAAFRYSETQ
                     LQSLLDQIVQVLAEEVSNEIKVDAGSPFFETKKHFGCGGNCHAIRQ"
     gene            40229..40567
                     /locus_tag="ACG842_000237"
     CDS             40229..40567
                     /locus_tag="ACG842_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbG family protein"
                     /protein_id="HFQ0246808.1"
                     /translation="MQLDNSEFEITKRRGLIVWVYSLKQLKNLKKFGLLHYVSRRMKY
                     VVLYLNEETLEETEERIRKLHFVRNVERSYRPDIEMNFAEKIGNKTVLKDEGFEIEEL
                     NTEIRLAEHL"
     gene            40686..41246
                     /gene="rsmD"
                     /locus_tag="ACG842_000238"
     CDS             40686..41246
                     /gene="rsmD"
                     /locus_tag="ACG842_000238"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HFQ0246809.1"
                     /translation="MRIISGNYGGRRLKSLAGANTRPTTDKVKESIFNMIGPYFDGET
                     VLDLFAGSGGLAIEAVSRGCSHAVCVDKNYQALKIIKENIEITKEPEKFTTIKLDADK
                     AIERLAAEKQQFDYLFLDPPYAKQKIIDQIHKMEELGLFSKTAVIVCETDKTVELPET
                     VAGFHQIRKQIYGITAVTIYRKEGNQ"
     gene            41243..41734
                     /gene="coaD"
                     /locus_tag="ACG842_000239"
     CDS             41243..41734
                     /gene="coaD"
                     /locus_tag="ACG842_000239"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HFQ0246810.1"
                     /translation="MKRALFPGSFDPFTKGHLDTVERAAKLFDEVVIGVFINTSKKSL
                     FPPEERMTLITKAVSHLPNVKVMHQENQLTVETAKEIGADALIRGIRSIKDFEYEREI
                     AQMNHHLYDELETVFLLAKPEYSHVSSSILKEVLHFGGDVSSYLPPVINEALAGKRES
                     NES"
     gene            41724..42767
                     /locus_tag="ACG842_000240"
     CDS             41724..42767
                     /locus_tag="ACG842_000240"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SepM family pheromone-processing serine
                     protease"
                     /protein_id="HFQ0246811.1"
                     /translation="MKAKQWLKTVLIIIFGMLIIGFVVVPLPYYVESPGATIDLKELI
                     TVNDKEDKGSGSFSLTSVGIRQATGFLALKAKMTPFEELISEEELTGGATNEEYNQMQ
                     QYYMDSSQNAAIEQALKLADKPYKMEFKGVYVMSVEKTSNFFGKLSVGDTVTKVDGKS
                     FQSTEAFMDYVKSQKVGQTIEISYLHNGEEKTASGDLIELPTDKKAGIGITLTDHTEI
                     ESDTDVRINSGSIGGPSAGLMFTLEIYEQVTGKNLRHGKQIAGTGTINSEGEVGRIGG
                     IDKKVASADKAGVEIFFAPDDTISDDVKKEYPEIKTNYEEAKAAAKKLNSSMKIVPVK
                     TVQEALDYLEKMK"
     gene            42926..43654
                     /locus_tag="ACG842_000241"
     CDS             42926..43654
                     /locus_tag="ACG842_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016925838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HFQ0246812.1"
                     /translation="MKGVMNDFYWSFFEKRTMIEKISKKILASPKLIIWMSMIVLIFC
                     IASGFLLSNLLFYRETEETAAKEILSSFSSSGEDPSAEGNDVEEDARPKVMYTDIKGS
                     VKEPGIYSFSSEERVYDVLKRAGGLLEEADSDRINFSAKIEDQQVLYIPAVGEELPEH
                     LDQLASSEGKQPTADSEPPKININTATPSELQQIPGIGSVKAQEIIRFREENGSFQKV
                     EDLQEISGIGEKTVEKLKNFVTIK"
     gene            43770..44015
                     /locus_tag="ACG842_000242"
     CDS             43770..44015
                     /locus_tag="ACG842_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HFQ0246813.1"
                     /translation="MIVRKETLKKPMLNVYLQNKISGIHIMNTAVSGNNSQALRERFA
                     KDVLSYTADKVFILIGTNDLAEHKQLSKETYQKICSG"
     gene            44012..44230
                     /locus_tag="ACG842_000243"
     CDS             44012..44230
                     /locus_tag="ACG842_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HFQ0246814.1"
                     /translation="MIVQLKKDYSSDHAFFITPPAVDETKQKYRNNKLIKEYISVLTE
                     VAKRHDCEVSIFIRNFLNKWIRRIFIMG"
     gene            44366..44905
                     /locus_tag="ACG842_000244"
     CDS             44366..44905
                     /locus_tag="ACG842_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComE operon protein 2"
                     /protein_id="HFQ0246815.1"
                     /translation="MLEKSSKACDTNKEKVTDKSHNERIPWDQYFMAQAVLLSLRSTC
                     TRLEVGATLVREKRIIAGGYNGAVSGDVHCIDEGCYVVDGHCLRTIHAEMNALLQCAK
                     LGIPTEGSEIYVTHFPCLACTKALLQAGVKGINYLHDYRNDPYAQALIEQLGVSVHQV
                     ALDSQHFSKLQNELTGKHV"
     gene            44898..47171
                     /locus_tag="ACG842_000245"
     CDS             44898..47171
                     /locus_tag="ACG842_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0009290 - DNA import into cell involved in
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HFQ0246816.1"
                     /translation="MSSVLERHVRNRLIFPAITSMIICLLYYTDNWGYRLLLAGIFLW
                     FSLKEGRFIAYLCILCSITVSISYRLQEYSFLEEKTTSAQITVFTDTIRANGNFLTME
                     GRLSENKQKLQLSYQVNTEAELKTWLNFRGKGIELTAEGEFISPAKKRNLYNFDQEAY
                     FRNHRISGRFQIQKIEQITMQNHWRNWLQRKRGSFISFVQSEFPQKTSVYINSLLWGY
                     KDAGFREPEEIFRESGLLHLFSLSGLHIQFYLGWLYYLFRRIGWPLHVSIIPLSLLTI
                     CYVSIAGSSISVLRASLLFLLRLLVKLLNKHYSTMDLFAVVFWLLLLFDPRFLFHAGG
                     QLSFFMSFLFILISFDGDWLKKTASHVLFTMITMPLIVWHFYEWPILGSLFTLLIAPV
                     FKFLFLPGVLFLTLFNRWLPETLLLLIEEGLILFERIIDFSSGSSLIIGRLPLVLSGM
                     YIFGLLICMDRLKEQPVKHLFYVGLFSFIFLTLPFLRFSSTIAFIDVGQGDSIFLQSP
                     FRKETILIDTGGQLHFEREKWAQGMIRPPAERNIVPYLKGQGISVIDKLILTHDDTDH
                     VGELPTLSQHFTIKKIYIGWGAGRNEPLQRHLSEAQKNGTEIIEIKHGDRISGYYDLY
                     VLTPFEKGEGRNEDSIGLWMEYNNRRFLFLGDLNQAMEKQLLLIYPNLKADVVKLGHH
                     GSRTSSNTDFLSHIEAKHGIISCGVNNRYGHPHSEVLETLEENQIKTWRTDQQGMITY
                     RWHPVFHPHGLVETMID"
     gene            47209..48255
                     /gene="holA"
                     /locus_tag="ACG842_000246"
     CDS             47209..48255
                     /gene="holA"
                     /locus_tag="ACG842_000246"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HFQ0246817.1"
                     /translation="MVSNLSIQAELQKIRKEKLASCYLILGTEKFLQDQVRTEIVKKI
                     QLEGEDDLNFLSFDMENASLDEVVAEAETLPFFGDQRLVFVENPYFLTGEKVNNGIEQ
                     NTDLLTAYLKEPLESTVLVFFAPYEKLDERKKVTKQLKKTAIIINVQQLNEKEVRQYL
                     MNTLENSSIKMERSAIDLFLRLTDLDLSKMMRELQKLVLYGQNQQQITVREVEELVPK
                     TLEHNIFDMTQYILKGKTEQALRLYEDLVMQGEETIKINAILLSQLRLFLQTKFLVKI
                     GYQQANIAETLKIHPYRVKLAMQEVKKFDEHLLVQLFDQLVEMDYQIKTGQIEKELSF
                     QLFVLRTGQLLRNR"
     gene            complement(48344..48598)
                     /gene="rpsT"
                     /locus_tag="ACG842_000247"
     CDS             complement(48344..48598)
                     /gene="rpsT"
                     /locus_tag="ACG842_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HFQ0246818.1"
                     /translation="MPNIESAIKRVRTSENANVKNSSQTSAMRTAIKKFEDAVASGAD
                     NVDALYKEAVKAIDMAESKGLIHKNKANRDKSRLSKKIAK"
     gene            complement(48998..49267)
                     /locus_tag="ACG842_000248"
     CDS             complement(48998..49267)
                     /locus_tag="ACG842_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0246819.1"
                     /translation="METFEGLLIDTYVLQEQPMMLTGINLQLEDRVINGLLKDRYLAK
                     YILRLPLNKYVLEVSGKWNRRNQLVIKKMLIKNIDEYIKIIGTPE"
     gene            complement(49529..49657)
                     /locus_tag="ACG842_000249"
     CDS             complement(49529..49657)
                     /locus_tag="ACG842_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0246820.1"
                     /translation="MFEKLKYFIGTIGWFLLAVLLILGLAYLLFPDLIQNVFDTAF"
     gene            complement(49737..50192)
                     /locus_tag="ACG842_000250"
     CDS             complement(49737..50192)
                     /locus_tag="ACG842_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HFQ0246821.1"
                     /translation="MESWLFLGIILLVAILAKNQSLMIAAAFVLIMKLIPASRSFFPW
                     LQGKGINIGVTIITAAILIPIATGEIGLRDLLEAFKSPIGWVAVFCGGLVAVLSSKGV
                     GLIASDPQITVALVFGTILGVVFLRGIAAGPVIASGITYCILQVFALGK"
     gene            complement(50330..52258)
                     /locus_tag="ACG842_000251"
     CDS             complement(50330..52258)
                     /locus_tag="ACG842_000251"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFQ0246822.1"
                     /translation="MTNQKKAFAATIGCLLFMIFGFIFDRFSLTITPLFYISAIIIGG
                     YKQTSEGLKELWNDRTLNVDLLMALAAIGACLIGNYFEGAMLTFIFCLSGALEEYTTN
                     KSQKEITALMNLQPQKAQLLKENGQMQEVDVSQLKVDNLVFVAKGAAVPIDGFLESTQ
                     ATIDESALSGESVPVEKYLGDEMYAGTLNQGNPITIRVTKTSSETVFAKIIQLVEEAQ
                     NTPTQTASFIERIENNYVKLILLAVPLMILLPHFFLGWSWDESFYRGMVLLVVASPCA
                     LVASATPASLAALSNSAKNGILIKGGIHLEKLSELKAIAFDKTGTLTKGKPVVTDSFF
                     FDEKDLAQQLLVAMEQKTTHPLAHAILQYFDCTIPEEIQYLPIEEITGFGLQTTYLGA
                     QWKVGKHAYDPETMIISKEIAEMIERLENQGKTVIYLSKDQQLIAVLGLLDIPKANTQ
                     QVISYFKSQNIHTSMITGDHSGTAKAIAEQVGIEHYYASCTPEEKTQLVKKENELYQV
                     NAMVGDGVNDAPALAAASLGVAMGQGTDAAMDIADIVLMKSDLNKFVYSHRLSLKMRR
                     IIKQNIIFSILVILLLIASNFLQFLNLPLGVVGHEGSTILVILNGLRLLRPLPDLSDS
                     SKKCKSCPLYKARLSSVK"
     gene            complement(52455..53288)
                     /locus_tag="ACG842_000252"
     CDS             complement(52455..53288)
                     /locus_tag="ACG842_000252"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HFQ0246823.1"
                     /translation="MFFANIIKAVILGIIEGITEWLPISSTGHLILADEFIKLDASSQ
                     FMSMFNVVIQLGAILAVVVLYFHKLNPFSPSKSTVEKKDTWTLWFKVIVACIPAIIIG
                     LPLDDWLEANFHKFLPVAIVLIVYGIAFIIVEKRNKNKTPRWTSLNDFTYQAALLVGL
                     FQVLALVPGTSRSGATILGSILIGASRFVATEFSFFLGIPVMFGASGLKIVKYLAEGN
                     SFKMEETVVLLVGTFVSFVVSVLAIKFLLSYLKKNDFTAFGWYRIILGIILIGYWFIA
                     M"
     gene            complement(53480..54184)
                     /locus_tag="ACG842_000253"
     CDS             complement(53480..54184)
                     /locus_tag="ACG842_000253"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HFQ0246824.1"
                     /translation="MTETTLYIVRHGKTMFNTIERVQGWCDTPLTKQGQEGIHYLGRG
                     LKNVDFSFAYSSDSGRAIETARIILGEHSKGKEIPYFIEKRIREWCFGSLEGGYDMEM
                     WGVIPRVLNFKTYDEMFTTEVSFEQIANAVYNLDTAGWAETYETLRDRVWTGFEDIAH
                     HCEKNGGGNVLVVSHGLTIAFLLNLIDPKQPVRAGLLNGSVTKVTYANGRFSIHGIND
                     TSYIDNGKTHSRDSFL"
     gene            complement(54207..54833)
                     /locus_tag="ACG842_000254"
     CDS             complement(54207..54833)
                     /locus_tag="ACG842_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFQ0246825.1"
                     /translation="MHIERLKTGMIEENCYLVYNEEALLIIDPGEDAERIKQEIKKTN
                     QQPVAILLTHTHYDHIGAVEPLRAYYQIPVYVSPLEQKWLGDPILNLSGLGRHDDIAD
                     VIVSPAEYEFEMKTYTLGNMSFRVVPTPGHSIGSVSFIFDDFVVSGDALFRGSIGRTD
                     LYTGNLEQLLHSIRTQLFVLPDEFAVYPGHGDATTIEQEKRTNPFFNR"
     gene            55159..57534
                     /locus_tag="ACG842_000255"
     CDS             55159..57534
                     /locus_tag="ACG842_000255"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HFQ0246826.1"
                     /translation="MIKQSKISVRHLVEFILRRGSIDNRKKSNHTALEGARIHRKLQK
                     DAGENYEKEVFLKTTVQLDEYYLIVEGRADGIFKRDGIYYIDEIKTSEPRFEDLEPEQ
                     VELFFHQARVYAYIYSQEHDLQEINLQLTYFQTAEEVITRKVEHQTREQLETFFKKLT
                     EDYKEWLVFQENWRTVRNASLMALKFPHDEYRKGQRELAVAAYKTIRTKQKLFVEAPT
                     GTGKTISTLFPALKAVGEEEGEKIFYLTAKTITRQVAEDAMTALKDTGAEVKSVTLTA
                     KDKICFLDETTCNPDQCPYANGYYNRINEGLWDLLNHENQITREVIETYARKHTLCPF
                     ELSLDVSIWCDVIIGDYNYLFDPTVYLRRFFEDEKNEDYLFLIDEAHNLVNRSREMYS
                     AELSYEKTKRSKEAIPKEFKKLHRRFNKLLKEFDSIREIAKEDHWDYHHQKAPAESLV
                     KAGYQLSEKIKEWLAEFPEHPSQEQLLPYYFDLLHFLKVSEFYDDHYETTVEKTYRDL
                     IVKEFCIDPSLFLEQSLDKGKSSILFSASFSPLSYYQETLGGQKSLAYKLPSPFPEGN
                     QQVFIANYLETTYRKREHSLAKLVETIHAFADGKVGNYFVFFPSYQYLDLAVEAFRQQ
                     YPDSNVLIQETDMNEEERERFLTKFQTNPAETLIGFCVLGGIFSEGIDLRGDRLIGSM
                     IVGVGLPQMNHEQELIKSYYDEKEHLGFAYAYQLPGMNKVLQAGGRVIRGMADQGIVV
                     LADQRFSSFSYRRLFPQHWQTAREVRSVGELEEGIQRFWLSKEAVNQKNEE"
     gene            complement(57558..58049)
                     /locus_tag="ACG842_000256"
     CDS             complement(57558..58049)
                     /locus_tag="ACG842_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HFQ0246827.1"
                     /translation="MAKDASFDIVSEVNTEEVKNAIQQTTKELSNRFDFKDSTVEIKL
                     ENSNLVVLSDDDFKIEQIKDVLFGKLIKRNVPIKNIHFSESEHALGGKARQTADLVNG
                     IDRENAKKITTAIKNEKLKVKAQIQEDQIRVTGKNRDDLQAVIALLRKLDLPIELQFT
                     NYR"
     gene            58236..59156
                     /locus_tag="ACG842_000257"
     CDS             58236..59156
                     /locus_tag="ACG842_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFQ0246828.1"
                     /translation="MTAYHKNERLKKIVVILITGLTAAVALNYFLIPANVFSAGMNGI
                     AQIISSLLYEWFHIKIDTGTFIFLLNIPVFVLGFLKVGKRATILSFVNVVCVSVMTML
                     LPVGQITDNILMNALVGGVLLGLGVGLSLKMGFTTGGMDIISLVLSQTTGKTVGNYML
                     ILNGIIVVIAGFFFNWESALYTIISIYAMTQVIDAIHTSHQKVTAMIVTVKPEEVTEE
                     LAKRMVRGMTLLPSVGGYSKKQGNMIMMVITRYEMYDLDQIVHEIDDDAFINILPTHS
                     VFGRFANENEQKMYRTTGVLPEIKNRSQKK"
     gene            59310..59654
                     /locus_tag="ACG842_000258"
     CDS             59310..59654
                     /locus_tag="ACG842_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HFQ0246829.1"
                     /translation="MSEANQERSGQEIELIKERILAALEMVIDPELGIDIVNLGLIYD
                     IEFNPENGETVIKMTLTTMGCPLADILTESIHDALKEVPEVTKAEVKLVWYPAWTTDK
                     MSRYARIALGIR"
     gene            complement(59844..60344)
                     /locus_tag="ACG842_000259"
     CDS             complement(59844..60344)
                     /locus_tag="ACG842_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0246830.1"
                     /translation="MKLIKLWEADLKKAYQLQTSFGPNESGFINNAYGYTFAEFLDYV
                     KKCEKTSLGIDLPESYVPATVFILESDEHEYVGIFNLRHKLNETLRNGAGHIGYGISP
                     NYRKKGYATQGLALVLQEAKKIGIDEALLSVNKKNLGSLKAQQKNGAAIYREDEKEYY
                     TKIKLM"
     gene            60872..60955
                     /locus_tag="ACG842_000260"
     tRNA            60872..60955
                     /locus_tag="ACG842_000260"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:60906..60908,aa:Leu,seq:cag)
     gene            complement(61279..>61713)
                     /locus_tag="ACG842_000261"
     CDS             complement(61279..>61713)
                     /locus_tag="ACG842_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HFQ0246831.1"
                     /translation="YSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEPVQAEQPVVQET
                     VQTETQAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTATNGRYIGRYQL
                     DSSYLNGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
BASE COUNT        20242 a        10663 c        13216 g        17592 t
ORIGIN      
        1 caaaaaagaa gcagatccgt tagcggttct gcttctttta gtttattata ttttcttatc
       61 aataatagga acctgatttt aaaagcggct acctctttac cgtgcctctt tacatacaaa
      121 aaatagagga attaagcgta aatggcttgg ctattataac taaaatgctg gttatggaga
      181 tgttgcagca agttttcgag aaaagcgtat tccatctctt gtccattgaa taagatctgg
      241 atacgataat gatttttgaa acgcttctgt tcatgaatct gactgattga aagttcgcct
      301 ttgtgttctg tctgataaag aaaagtcctt ttacggcatt cagcacagcg cttggttgct
      361 cggcggattt ttcggaatgt ataaaaacta gtatctttat tgcataaata aacgtctttg
      421 atcgacagca aatcattata tagacaggaa cgttcttcag ctgttaattc tgataaaaaa
      481 agctgacctt gttttattgc atcttccatc ggcaagtgcc tcctcgaacc cattatccaa
      541 atttttagtt aattctaata taccatcatt aagtataaat gtaaacgctt gttgatggta
      601 taagattttc gtttctgata aaacacgaac attaaaagaa tctaatgcct taaatgtaca
      661 tatttttgtt gatttataaa aagatgattg ctaaaattta aagtgaaaaa acgaacaaaa
      721 aacgatgtat ttgcttttaa tgtaaataaa aaggagagac gtacatggag aagaaagaac
      781 ttttgtatga aggaaaagcc aaaaaaatat atcttacttc tgatgagaac ttgatttgga
      841 tagaatattt agatcaagca actgcgttga atggcgcgag gaaagaccag ataagtggaa
      901 aaggagaatt gaataatcaa atcacaagtg caattttccg ttatctgact caaagaggaa
      961 taaaaaatca ttttattgaa caattgtcta aaaccgaaca attagcaaaa aaagtacaaa
     1021 ttatccctct ggaagtggtt atccgaaata ttgcagcagg aagtttctca aaacgtctag
     1081 gtgttcccga aggtaaagaa ttcatcaaac caatcattga attctactat aaggatgacc
     1141 agttggatga tccatttatc aatgaagagc atatctattt cttacaactt gcaaagccgg
     1201 aagaaatcag tgagctgaaa gcccaagcgt tggctattaa tgaaattttg accgaattat
     1261 ttgctaaggt cggtttggtt ttagtagatt tcaagctaga atttggccgg tcgaatggac
     1321 aaattttgct tgcggatgaa gtaacaccgg atacttgccg attatgggat gaacgtacga
     1381 atgaacatat ggacaaagac gtttaccgca gagatctagg tgatttgatc ccagtctatc
     1441 aagaagtata taaccgtcta tcggaactaa ctgaagaaaa ggagcgagta tgatgtattt
     1501 cgtaaaagta tatgtaacct ataaagattc agtgttagat cctcaagggg aggcagtaaa
     1561 aaaagcagtc catcgtatgg gatatgaaac aatcgaagat atccgaatag gaaaatactt
     1621 tgagatacaa cttgctgatg cagaagaacc aatcgataca gtgattgaaa cgatttgtga
     1681 caagctgctt gcaaatgtaa atatggaaac ataccgatat gaaatcaaaa aaatggagga
     1741 agtataacat gaaatttgcg gtcatcgttt ttccaggttc aaattgtgac gcagatatgt
     1801 tatgggcagt tcgcgagatc atgggcgctg atgccgaatt tgtacggcat gatgagtcct
     1861 ctttagaagg atttgatggg gttttactgc cgggaggatt ctcctatggt gactatttga
     1921 gatgcggtgc gattgctcgg ttttctacaa tcatggaaga agtgattcgt tttgctgaag
     1981 aagggaaacc agtattcggt acatgcaatg gattccagat attgacagaa gcaggacttt
     2041 tgccaggtgt actgagacga aatgagtctt tgcattttat ttgcaaaact gtaccattga
     2101 aagtcaaccc caagacggcc tttacttcag agtatgaagc cgacgaaata atctatcttc
     2161 cagttgctca tggggaagga aattatttct gtgacgaaga aacacttcaa gaactaaaaa
     2221 aaaataatca gattgccttt acttataatg acaacatcaa cggcagcgtg gaaaacattg
     2281 cagggattac gaacaaagca gggaatgtgt taggaatgat gcctcatcca gaacgtgcag
     2341 tagaagactt gctaggatca gaagatggga aacggttctt tgcttccatt ttaaaaaact
     2401 tcggaaaggt gaccgtatag atgaaaacat tgaaagaacc gacagctgaa gagatcaaga
     2461 aaaaaagaat ctatgccgaa tggggattga ctgatgaaga atatcgaatg atcgaagaag
     2521 atatcttagg aagaatgcct aattatacgg aaactggact gttttctgtt atgtggagcg
     2581 aacattgttc ctataaaaat tctaaaccgg ttctacgcaa atttcctact agtggacctc
     2641 atgtgttgca aggccctgga gaaggcgcag gcatcgtcga catcggtgat ggactagcag
     2701 ttgtcttcaa agcagaaagt cacaatcatc cttcagccgt ggagccttat gaaggagctg
     2761 cgacaggagt tggcggaatc atcagagata ttttcagcat gggggctcgt ccaattgcgt
     2821 tgctagattc attgaggttc ggcgaattag ataatccaag aacgaaatat ttactagaag
     2881 aagtagttgc aggtatcggc ggatacggca attgtatcgg cataccgact gtaggcggcg
     2941 aaattgcatt tgatccttgt tatgaaggaa acccattagt caatgcaatg tgcgtaggtt
     3001 tgattgaaca caaacatatc cagaaagggc aagccgctgg cgtcggtaat tcgatcatgt
     3061 atgtaggtgc aaaaacaggt cgcgacggta tccacggagc aacttttgcc tcagaagaat
     3121 ttaatcaaga agaagaacag caacgttctg ctgtacaagt aggcgatcct tttatggaaa
     3181 agctgctgct tgaagcgtgt ttggaattaa tcttgcatca ttcggattct ctaataggta
     3241 tacaggatat gggagcagca ggacttgttt catctagtgc ggaaatggct tcaaaagctg
     3301 gctcaggact tatattgaca ttagatgaag tcccacaaag agaaactggg atgacacctt
     3361 atgaaatgat gttatcggaa tcacaagaac ggatgttgat ttgtgttaaa tcaggagagg
     3421 aagaaaaaat ccaagaacta tttcagaagt atgatctaga tgcagtcact atcggtaaag
     3481 taacagacga cgggcaatat cggctatatc atcaaggaga agaagtagca aatctgccag
     3541 tcgatgcatt agctgaagat gcaccagttt atcataaaga aatgaaagaa ccagctcgaa
     3601 tcgtagaatt tcagcagtta gctccttatc agccagttat cgaagaacct ggacaagtat
     3661 tgcttgatct gctgcagcaa ccgacgattg cttcaaaacg ttcaatctac gaaacttatg
     3721 attcacaagt acaaaccaat acagttgtcc gtccaggaag cgatgctgct gtgatgaggg
     3781 ttagagggac gaataaagcg ttagctatga ccacagactg caacgcgcgc tatctgtatt
     3841 tggatccaaa aatcggagga caaatagcag tggcagaagc tgctagaaat attattgcta
     3901 gcggtggtaa gcccttggcg atcaccgact gcctgaacta tggttctcct gataaaccag
     3961 aagttttttg ggaattatcg acttcagctg acggaattgc tgctgcgtgt gaaacgttag
     4021 gaactcctgt tatttcagga aatgtctctt tatataatga aacggatggt caagcaatct
     4081 atccaacacc aatgattggt atggtcggat tgattgagga tcataaacat atcactactc
     4141 aggaattcaa aaaatcaggg gatctaatct atatattagg taagacgttt gcagattttg
     4201 atggcagtga attacaaaag atgcagcttg gaagaatcga aggtgtgatc cgaaactttg
     4261 atctatccat tgaaaagaga aaccaagagt tagtattgac agccatccaa aacggattga
     4321 ttgaaagtgc tcatgactgt tcagaaggtg gcttggcaat tgcactggca gaatcagctt
     4381 tcaaacatca gctagggata tctgtacagt ttgaattgtc ctctgctcaa ttatttgcag
     4441 aaactcaatc aagatttgtg ttgacagtcg caccggaaaa taagacacga ttcgaagaaa
     4501 tgatgggaga tgctgctgtt ttagcaggta aggtgacgga tgaagcgata atcgaaattt
     4561 cagcaactga cggtcagatc aaaatagaaa ctgcagtagc aagaaagtgc tgggaggatg
     4621 caatcgtatg tcttatgaag tgaagagttt aaatgaagaa tgcgggatct tcggtgtttg
     4681 gggtcatcca gacgccgcta gtgtggcata cttcgggctc catagtcttc agcatcgagg
     4741 acaagaaggt gcaggtatcg tgtccaatga tcgaggaaag cttaaggggc atcgagattt
     4801 ggggctgctt tctgaggtat tcaaagatca aagaaaattg cagcagttaa caggagaagc
     4861 tgcaatcgga catgtacgtt atgcaacagc aggcaatgga agtgtggaca atattcagcc
     4921 ttttctattc aaatttcatg atcagtcgat cggcttagcg cataatggta acttgatcaa
     4981 tgctcgttcc cttagaaaag agttagagga aaatggggcg atcttccatt ctaactccga
     5041 tacggagata ttgatgcatt tgatccgacg aagtgaagaa acagattttt taaataaatt
     5101 aaaaagtgct ttgaatgaag tcaaaggtgg ctttgcctat ttattaatga cagaaacggc
     5161 aatgattgcc gctttggacc caaatggttt tcgtccatta gcaatcggac aaatgaaaaa
     5221 cggagcatac gtgattgcct cggaaacttg tgcattagaa gttattgggg cacgttttat
     5281 aagagatgtc cagcctggtg agatagtgat catcaatgac gaaggaatcc agattgattg
     5341 ctttacgaag gacacgcagc tttctatttg ttcgatggaa tatatctatt ttgccagacc
     5401 agattcaaac atcgcaggga tcaatgtgca tactgcacgg aaaaatatgg gacggaattt
     5461 agcgaaagaa tcgccagtaa aagcagatat ggtcgttggc gtacctaatt cttctttgtc
     5521 agccgcaagc ggttatgcgg aagctagtgg aatcccttat gaaatcggat tagtaaaaaa
     5581 tcaatacatt gcccgaacat ttatccagcc aacgcctgag ctaagagagc aaggtgttcg
     5641 aatgaaatta tctgctgtac gaggagtagt ggaaggtaaa aaggtgatta tggtcgatga
     5701 ttcaattgtt cggggtacga caagcagacg gatcgtccat cttttgaaag aagcaggggc
     5761 aaaagaagtc catgtgagga ttgcttctcc tcctttgaaa tatccatgtt tttacgggat
     5821 tgatattcaa acgcgtaaag aactcattgc ggcaaatcac acgattgatg aaatcaaaga
     5881 atgtatcggt gcagattctt taggtttttt aagtgaagag ggtctgatcg aatcgatcgg
     5941 tctggaaaaa gatgctccgt attcaggatt gtgtatggct tatttcaacg gagattatcc
     6001 gacgccgtta tatgactatg aagaaaaata tcaggaatca ctaaaagaaa aagtatcgtt
     6061 cttttaaaag cagaccaaga agggagtata tgaattcaat ggcaaatgca tacgcaaaag
     6121 ctggcgtaga tgtcgaagct ggatatgaag tagtagaacg gatcaagaag catgtcaaaa
     6181 aaacggaacg tttaggtatg atgggcgcta taggaggatt cggcggttgt tttgatttat
     6241 cggtctttca attaaaagag ccagtccttg tctcggggac cgatggcgtg gggacgaaat
     6301 tagcagtggc aatcgaagca gacaaacacg atacaatcgg gatcgactgc gtagcgatgt
     6361 gtgtcaatga tatcttagca caaggcgcag agccgttata tttcttagac tacttagcaa
     6421 cggggaaaaa tgatccgtta cgtttggaaa atgtagtaaa aggtgtagca gatggatgtg
     6481 ttcaaggaaa tatggcgctg attggcggag aaacagctga aatgcctggt atgtactcag
     6541 gtaatgatta tgatctagcc ggatttgctg tagggatagc tgaaaaatct caattattga
     6601 caggagaaac gatcaaccca ggagatgtgt tgcttggaat agcatctagc gggattcatt
     6661 caaatgggta ctctcttgtt cgcaaaattt tctttgagca gcagcagttt tctttatcat
     6721 caaaactgcc agaattgaaa aaaacacttg gcgaagaatt gcttgagcca acgaaaatct
     6781 atgtttcttc cttgcttccg ctgatcaaag aaaagaaaat aaaaggagct gcacatatca
     6841 ctggtggagg gtttgtagaa aatatcccgc gaatgttacc agaatctatg gctgcacaaa
     6901 tcaaacttgg ctcatggccg attcttccaa tattcggctg tttacaaaaa tatggtgaaa
     6961 ttcctgagat ggagatgtat gaaatcttca atatgggcat cggtatggtc ctggcagtcc
     7021 aagaatcgaa agtaaaagaa atagtacacg aaattgagca agctggcgaa aaagtttatg
     7081 aaatcgggaa agtgatcgcc aaagaaaaac atcgtgtggt gtttatagga gaagaacgat
     7141 gagaatcgct gtttttgctt ctggaaacgg aagcaacttt caagcgctag ccgattatct
     7201 atcgaaaaaa ggattggaat cttcgattga ctggttgttt tgtgatcagc ctgaagcata
     7261 tgttttgaaa cgtgcaactg cgttgagtgt accggcagat tgtttttcac ctaaagaatt
     7321 tgattcaaaa aaagagtatg aagaagcaat tttgcataaa ttgaaagaaa aaaagatcga
     7381 tttgatcgta ttagctggat atatgcgaat cattggaccg gtattgctga aaaattatga
     7441 taaaagaatc atcaatatcc atccgtctct tttacctgcc ttccctggtt tgcatggcat
     7501 acgtgatgcc tttgaagctg gagtaaaaga aacgggtgtc acaattcatt atatcgacca
     7561 aggtgtagat actggaccaa tcatccggca ggaaaaagtg agaattgaac aggaggacac
     7621 gttcgattct ttagaagaaa aaatccatag agtagagcat cgaatctatc cagaagtgat
     7681 tagtgaaatt attgaaaatg gaggctatct gaaatgacaa gagcattgat cagtgtatca
     7741 gataaaactg ggattgtagc attcgcccaa ggtttgattg aacaaggagt agaaatcatt
     7801 tcaacaggag gaacaaagca tgcgttggat aaagctggga tcaaaacgct ttcgatcgaa
     7861 gaagtgacag ggtttcctga aatgatggat ggccgtgtca aaacacttca tcctaaaatc
     7921 catggtggtc ttttaggaag acgcgatctc cctagccata tggaggcaat ggaaacggaa
     7981 aatatccaac caatcgactt tgtttgtgtc aatttatatc ctttcaaaga aacgattatg
     8041 aaaccagatg tcgaaatagc agaagctatt gaaaacattg atatcggtgg tcctagtatg
     8101 ctccgtagtg cagcaaaaaa ccatgaattt gtcacctctg ttgttgatcc atctgactat
     8161 gaagaagtac tttcagaatt acgtcagtat gggcatactt cactttcttt cagacaaaaa
     8221 ctagcggcaa aagtctttcg tcatactgcg gcatatgatg cactgatcgc tcaatactta
     8281 acggattggg tggaagaaaa agaaccggaa aaattgacac ttacgtatga acgaaaacaa
     8341 acgcttcgtt acggtgaaaa tagccatcaa gccgctgcct tttatcagtc cgctcttctg
     8401 gaaaaatatt cgatcgcagc agcaaaacaa ctccatggga aagaactttc ttataataat
     8461 atccgagatg cagatgctgc gctacggatc atgaaggaat atgacgagcc aacggtagtc
     8521 gccttgaaac acatgaatcc ttgtggtatt gggacagcat ctacgatttt agaagcgtgg
     8581 gaagcagcgt atgaagcaga tcctgtttca atctttggtg ggatcatcgt tttgaatcga
     8641 gaagtagatc tgccaacagc agaacagatg cataagctgt ttctagaaat catcattgcc
     8701 ccttcttata cacaagaagc tttggaatta ctgcaaaaaa agaaaaacat ccgattactg
     8761 acagtagact ttgcgcagga agaaaatcaa aatcgagaag aaacggtctc agtcttaggt
     8821 ggacttcttg tacaagatca agatctggca atagaaaatg aagaagagtg gaaagtcgtg
     8881 acaaaacgta agccgactcc agcagaagca aaagcattag cgtttgcttg gagagctgtc
     8941 aaacatgtga aatctaatgc gatcgtactg gcaaacgaac gtcagacagt aggaatcgga
     9001 gccgggcaga tgaatcgagt cggttcagta aaaattgcga ttgaacaagc gcaagagaaa
     9061 atggaaggtg cagttttagc tagcgatgct tattttccaa tggatgattc ggttgaatat
     9121 gcagccaaac atggtataaa agcaatcatt cagccaggag gaagcatcaa agaccaagat
     9181 tctattgata tggcgaataa atacgatcta gcaatggtct ttactgatgt tcggcatttt
     9241 agacattaga gaggagtcgg ataaatgaac attttggtga tcggtgcagg tggtcgtgaa
     9301 catgcaataa gtaaaaaact tttagccagt ccgaaggtgg atacggtgta ttgtgcgcca
     9361 ggaaatccgg ggatgacaaa agatggtatc aattgtgtcg ctatcagtga gcgagatcat
     9421 tcagcgttaa tccattttgt taagaaagag gctattgatt ggagtatcgt tggtcctgaa
     9481 gttcctttgt taaatgggat cgtcgatgat tttatggcgg aaggattgaa aatcttcggt
     9541 ccgacaaaag cagcagctct gatcgaaggt tcgaaaagtt ttgctaaaca attaatggat
     9601 acttatcaaa tcccgacagc taaatccaga agttttgaat gttacgaaaa agcagcagcg
     9661 tatatcaaag aaaatggtgc gccaatcgta gtgaaagcgg acggcttagc agctgggaaa
     9721 ggtgtagtcg tggctaccac agtgaaggaa gcgctggatg cggcagaaca aatgttgaac
     9781 cagcatcgat ttggggatag tagcaaaaaa atcgtcgtag aagagttttt ggcaggagaa
     9841 gaattctccc ttttggcgtt tgtccgtaat accaaggtgt atccgatggt catttcgcaa
     9901 gatcataagc gggcatttga taacgataaa gggccaaata caggcggaat gggtgcttat
     9961 tcaccagtac cgcaaatttc aaaaaagatg attgaaaaag ctgtggaaga agttttaaaa
    10021 ccagcggctt tgggaatgaa agaactagga cgctctttca cgggtgtttt atatgcgggt
    10081 ttgattgcta cgaaggaagg accaaaagtg atcgaattca atgcgcgttt cggtgatcct
    10141 gaaacgcaag tcgttctttc tcggttgaag agtgactttg cgcaagtaat cgatgattta
    10201 ttagagaatc gtacagtaga gttaaagtgg cagcaagaag gatacaatgt aggcgttgtg
    10261 gttgcagctg ccggttaccc cgaagaatat gagacaggaa tggttctacc agattttctg
    10321 gaagaagagc tttctgttta ctatgctgga gtttcctcaa ataacggcaa actagttgga
    10381 gcaggaggac gtctttattt agtagaagca tacggtgaga cgatcaaaga agcacagaaa
    10441 aaaatttaca ccgcgttaat ggataaaacg acagatggaa cattttatcg tacagatatt
    10501 ggagaaaaag ctgccaatag cgaactgatc gtgaagtaaa tcagctgctt ccatttataa
    10561 aaaatacaac aaacataatc ggaagcgccg attttagtga agccttttca taaagagtgt
    10621 ggtacacttt tgttgtgtat ataaataagg aggagttcag atgaaaaaaa ttcaattagg
    10681 tacttctgat cagaaagcag ctgcagtgat cttaggctgt atgcgtatca acaatgcgga
    10741 agatccagta aaagtgattg agacagctta tgaaaacggc attgattttt ttgaccatgc
    10801 agatatttat ggaaacggtg aatgtgaaga aatctttgca gatgctctag caaaaacatc
    10861 aatcaaacga gaagatcttt ttattcaaac aaaatgtgga atcgttccag gtgtgatgtt
    10921 tgatttttct aaaaaacata tcattgaatc tgtagaaaaa agtttgaccc gtctgaaaat
    10981 ggattatgtg gatgctttac ttcttcatcg tccagataca ttggtagaac cagaggaagt
    11041 agctgaagcg tttgatgaat tggaacggtc aggaaaagtt cgttattttg gcgtcagcaa
    11101 tcaaaaacca atgcaaatcg aattgctgaa aaaatatgtt cgtcaaccat tagtagctaa
    11161 tcagctgcaa tttggcttga aacatactgg aatgatcgat caagggattc atgtcaatat
    11221 gacagatgaa gcaagtatcg atcatgacgg tagtgtactt gattattccc gattgcatga
    11281 tatgacgatc caagcatggt caccatacca gtatggattt tttgaaggtg tatttatcgg
    11341 gaacgaaaaa ttccctgaat taaaccaaac attagaagaa ctagcagcta aatatcaaac
    11401 gacaccaact ggcttggctt ctgcttggat tttacgccat ccagccaata tgcaagtgat
    11461 cgccggtacg atgaacgtag ggcgaatcaa ggaaatcgct caagcttcag aaattaatat
    11521 gtctcgggaa gattggtacg aattgtaccg tgctgctgga aacattttac cttaaagatt
    11581 gtcataagca ggtcaaaagg gctataaatt aaaaagacag aataacttga aagctgtttt
    11641 cagcttcgag atattctgtc tttttttatt gcaaatggaa agagtatcat tgtacggatt
    11701 tcgtttatta gttatttttg tttttaagga aaagctgtgt ccgatattct tttggagaaa
    11761 tcccttgttt tttcttaaat aaataactga aatagttagg gtcattataa ccaatttcaa
    11821 aagcaatatc tgaaatacga tagtctgatt gctgcaataa tttttttgct aattcgattc
    11881 tttgttctgt cagataatcg atgaatgttt ggttaaacga ttgagaaaaa atcgtactga
    11941 aatgagaggg acttaatgca acttgttgag cgacccaagt taaggacata tctggatcgg
    12001 taaaattttc tttgataaaa tccaatgcat ggttcaaaat tggctgatat ttgttccttg
    12061 aaggctgtga gggcagagca gttaagaaag aaatgaacat ataaactgtc gaacggtaaa
    12121 gcgagtcgtc ggcgctgatt gctaagcgtt gactggtgga tgtgcaatga accatttctt
    12181 gcgggatttt cttttctttc aggcagacat gctggagtag cttagtcaag acaaataaac
    12241 gatataatcg tgttcgagtt ggtgtgcctt gtggtttcgt caagtgttcg atgtacttag
    12301 atacttcgtt tttcgataat gaagacaaat caagtgagag gtcaaagtaa aagacagaat
    12361 caactggaag tttcgcttct tgaatcaaat cctcatagct gataatttgt tctttaaggg
    12421 aactggcggt ttcaagtatc atctgcgtga tctcaaaaga tgcaggtaat tcgctgattc
    12481 tttctgcgac aggaccaatg gcgacagaaa aattattttt ttgtcccttt tctaattcat
    12541 ggaccaacgt ttgggctacc tgataactgg tctcgatcac attttcttca agagaatcaa
    12601 ctattaaaaa tttgatgaat cgagaggaaa cagcagagaa gatcacttgt tgattctccc
    12661 taaataaaaa cttcaaataa tcactgagat gagcgtaatc ttcaaagtct gtactgactt
    12721 ggtttgctgc taataatacg gtcatttttt tccctgctaa agagcgattg aaagattcag
    12781 caagtttcaa tgtttcactg atcgtaagtg agcctttgaa caaaccgttc aagaaatgat
    12841 tttttttcaa ttcgaaaaca agatcagaag aagtgtgcgt ttctttttgc tgcaaatcta
    12901 aagcagttac tgctttttct attgcttgtt tcaattcgct acttttaatc ggtttaagca
    12961 gataatcatc tgcctgtacc tggattgctc ctttcatata atcgaaatcg tcgaatccac
    13021 taataaatat gagacgagtc caaggaagta gtttccttgc ttcttttgaa aaagtcaacc
    13081 cgtccatgaa aggcatttgg atatcagtca gaatgatgtc gggctgtaag tcgataattg
    13141 aagacagtgc catctcacca tctccagctt cgccgacata agtgattgga taataagaac
    13201 caaggacagt tatttgttcc cgcaagttat tgcgaatgag gtattcgtct tcgatgataa
    13261 aaactttata catgtttgtt tcctccaatt gttttgggag atagagggat catcagtgtg
    13321 acagttgttc cttctcggta tttgctgtca atcgtgatag gtttttgatc atttccgtaa
    13381 taaagaagga gacgcttgtt tacgttgtaa agtccgtagc cgatactgaa atctgaatcg
    13441 atccctttgg ctaactcttt acgtatttct gccagttttt ctgcagggat gccaatcccg
    13501 ttgtctataa ctttgaattg gatcatctca tcaattttgc atgtggtaat acgaataaat
    13561 cctttgtgac ggacgtactt cgttccatgg tatacagcat tttcaatcaa tggctgcaag
    13621 atcattttta aaatagaaac aggaaaaagt gtttctggta tatcgatctt atagtcaagc
    13681 atatggccgt atcttatttt taagatgact agataatcat tgatatggct aatttctttt
    13741 tcaagaggaa tccagtcttg tcctttgctt aaagacgtac gaaaaaagcc ggaaagtgca
    13801 tgggtcatag tcagagcttc ttcaaagcag tcttgttcga tcaaagaaat aatggcatcc
    13861 aaactgttat aaataaaatg gggagtaatc tgagcttgca atacacgaac ttcactttgt
    13921 gctaaatggt attgtttcaa agcgttctcc cgtaaagact ggtttagatc ttccgccatt
    13981 ttattcatgc tagcggtaag tgtttgtaat tccagtgttt ccggcagggt taaacgaaag
    14041 ccaaggttcc ctgaagaaag ttgttctgac atctcgatca gctgattgat cggttcttgg
    14101 acctttctct ctaaaaagcg acgattcttt ttcgcataga aaaaaatggt actaagaact
    14161 ccaagttcca ctattgttaa tagaatgagc gagcggacaa gtgtcgtatt ttttttgctt
    14221 gctaagtcga tctccacacg aacaaaatct tgtaagatat ctgacagcag ttgagtgaca
    14281 gaatctacct gattgatgat ctcttcattg gtatccataa aatcttttgc caagatatta
    14341 ttgatcagct cgttttgata atcttccagt gtatcgatcg tacgtagcga tacatctaac
    14401 gtactaattt ctttaagtgt atttgtatta tttttgatct ctcgaatatc attcctaagt
    14461 tcttctagga tattttcctc attgaactca tctacggtta cttggccaga aatcaagtcc
    14521 cacatacttt ccaaaacttt ttgatcaacc ttcaaagcaa ttgcatctgc tttttggata
    14581 tttgcgatcg tctgttgata aatcaaaagc tgttgcgaat ataacagtat gctcagtagc
    14641 aaagggataa cagcaaatag aagcatggct ttgtttgctt gggcaaaaat ctgttttatg
    14701 cttaaatttt ttgtcatgat acttctccag tcaataattt ctggttggga tgttagctat
    14761 attctgctgg gaaaagacag tctcggaaat aaaaatatct ttaggtaatg tttttcctgt
    14821 ttgaatattt ccaaaataac gagtaacggt attggctaca taccagccga catcaggttt
    14881 acactcgact acacaattga cgattcctga gcgtagcttc tcgatcatat cttcttgtcc
    14941 atcaatagtt acaatgatca aatcttctcc aggtttgatt gatgtttctt ttatcgcagc
    15001 taaagcaccg tgagtcattt catcactatg gctgtataat acatcgattt cggataaatc
    15061 atttgtttct aaatatcgtt tcatctggtc ttttcctttc atccgaataa aatcaccgac
    15121 aattcggtga acaatagtca tcggaggttt atggtcgatg gttccatctt tttttagggt
    15181 tggtttacga ttgattgatt ccaaaaatcc ttcggagcgt agcttggtcg gtgtggagtt
    15241 ctctgaacca gcaagttcta ggattctgat ttcctgtttc tttgaatttg tgaagtgatt
    15301 gctgacaaat aaacccgccc gatttccttc tgctttgaaa ctggggccaa tgtgggtgac
    15361 gaaaagctcg ggatcattcg tttgaatatg tctgtcgaca agtatgacag ggatccctgc
    15421 tcgctttgct tcttttaata cagagtccca cccggattct tgcaaaggag taaaaatgat
    15481 catatccact ttataggcaa tgaacgtccg tatatcttgg atttgccgtt cctgattcat
    15541 gtagccattt cggtagagaa cttggtaatc ttctttttca agcgcagtga taattgattc
    15601 agtatgcttt ttccgccagc tactctccgt tcctgattgc gaaaaaccaa cgactagtgg
    15661 gtcaggtgct tcctttgtat taggtgtaca ccccatcatt tggtaagcga ttacaacaag
    15721 ggctaaaaaa aataaaacac gtttcacttt aaatcactcc atctggattt gttagtacta
    15781 tcttatcaga taaaagggaa aatcaaagtc aaaaggaatc aatcgaaaaa aatccaagaa
    15841 acttaaaaaa atccaagaca aaaaaagtac acctaaaaaa tttaaagtaa atcccataaa
    15901 aaaatcaatg ttaacgcttt ctttattagc tatactcggt gtatcaattg aaatggggga
    15961 taaaaaatga aaaacaaatt tagaaagatt gttgggacta tcgctatttt gaccgcaagc
    16021 attctattaa gtgcgtgtgg caattcagga tctgcagatg atagtacggg gtatgtagga
    16081 atcgctatgc cgaccaaatc agcagaaaga tggatcgctg atgggaacaa catggtctcg
    16141 gaattagaaa aactgggtta taaaacagat cttcaatatg gagaagataa agtagaaaat
    16201 caagtagcac aaatagaaaa tatgattaca aaaggggtag atacacttgt gattgcttct
    16261 atcgatggtt cggctttaac tgatgtgtta gctaaagcaa aagaagcaga tatcaaggtg
    16321 atcgcctatg atcgtttact gatgaattca gaaaatgtcg attattacgc gacttttgat
    16381 aattttggag taggtgtatc gcaagcagct tatattgaag agcacttagg attgaaagaa
    16441 ggaaaaggac cattcacgat tgaattattt ggtggctcgc ctgacgacaa caacgcacta
    16501 atcaattaca acggagtgat gtctgtttta caaccatata tggacaacgg tcagttggtc
    16561 gtgccatctg gacagacgag tttcagtcaa atcgctacgc ttcgctggga tggttctacg
    16621 gcgcaagcaa ggatggataa tctgttaagc gcaaattata ctgaccaaac attggatgct
    16681 gttttgtcgc catatgatcc aataagttta ggaatcattt cgtcattaaa aggcgtgggg
    16741 tatgggagtg aaagtaaacc tttacctgtg attactggac aagatgcgac agttgctggc
    16801 gttaaatcga tcattgccgg ggaacaaaca caaacgatct ttaaagacac acgtatctta
    16861 gcaaaaaata cgattgaaat gatcaaagca atcagtgatg gagaagaggt tccggtcaat
    16921 gatactgaaa cttatgataa tggggtgaaa actgtcccta cgtatttagc aaatacagta
    16981 tctgtcgata aagataatta tcaagcagag ctgatcgaca ccgattatta taaagaatcc
    17041 gatctgaaaa attaagagga accaaacaag gaactaaaga gatctaagtc agagagagga
    17101 taagaattat ggcggattgt attttaaaaa tggcagatat caccaaaaaa ttttctggaa
    17161 tccgagcgtt agattgcgtg aatctgatga ttgaacgtgg ggaaatccat gcattgtgcg
    17221 gagaaaacgg agcgggaaaa tcaacattga tgaatgtctt gagcggcttg tatccatatg
    17281 gctcctatga cggggaaatc ttttatgatg gaaagctgtg tcaatttaaa gatttgaaag
    17341 atagtgaaga aaaaggcatc gtcatcattc atcaagaatt agcactaagt ccttatcttt
    17401 caataaaaga gaatattttt ctcggaaatg aacaagcaga aagaggcgtg attgattggg
    17461 aactgacaga aaagaaaaca tatgaactgc tgaataaagt cggtttgtct atcgacccga
    17521 atcttctagt atcccagatt ggcgtagggc aacagcaact agttgaaata gctaaagcat
    17581 tatcaaaatc cgtccgtctg ttgattttgg atgagcctac cgctgcattg aatgaagaag
    17641 aaagcgcgaa tttgctgaga ttgatccgag agttgaaaga gcaaggtgta acctccatca
    17701 ttatttcaca taagctgaat gaaatcgtgg atatcgcaga tcgaatcacc attttacggg
    17761 atggacagac gattgagaca ctagaaaatg aaatgatcag cgaagagcat attattcgcg
    17821 gaatggtggg cagagattta gttgatcgtt atcctgaaag aaaaccggat attggagaag
    17881 tttattttaa agtagaagac tggacggtct atcaccctct tgatcacgag cgaatcataa
    17941 acgaccacat caacttgcat ttgaaaaaag gagagattgt aggaatagct ggactgatgg
    18001 gagcgggcag aactgaattc gctatgagtc tatttggaca ttcttatgga agcaagatat
    18061 ccggacgtat attaaaggaa ggggcagaga tatcagttaa gaccgttcct tcagccattg
    18121 caaacggact cgcttatgtc tcggaggacc gaaaatcttt aggactgaat ctgctgatgg
    18181 atattcggga aaacgtgacg ctgagcagtt tggataaaat aagtcaaaaa ggagtattag
    18241 ataaagaaaa agaagtaact gaggcagaaa atttccggaa aaagatgcgt ataaaaacaa
    18301 actccatcca tcaaattgtc agtagtctta gtggtgggaa tcaacaaaaa gtcgttttat
    18361 ccaagtggct gatgacacaa cccgatattc tttttttgga tgagccaacc cgtgggatag
    18421 atgttggggc aaaatatgag atctacacga ttatcgaaga aatggcagca aatggcaaag
    18481 ctgtctgtct tatctcttct gaacttcctg aaatattagg gatgtgtgat cgaatctata
    18541 cgatgaacga aggacgtatt acaggagaag tctcgcgaga agatgcagat caggaagtct
    18601 tgatgaaatt aatgacgaaa gaagaggtgg gttaagttgg aacaggcaaa aaatcttacg
    18661 gataaaaaag agaaaaaact aaatttgaaa agtagagcag ctgatctttt tggtcgctac
    18721 agcatggtga tcattttagc gggactactt attgcgtttc aattgatgac ggatggtatt
    18781 ttctggcgtc cattgaatat aactaacctt gttctgcaaa atagccatat attagtacta
    18841 gctgcaggga tgttattagt tgtgctctta ggtcatgtgg atttgtcggt agggtcagtg
    18901 atggcgtttg ttggtgcaat tgcgggcatg atgatggtca atcgccaaat gaacccgtgg
    18961 atgacagtta tcgcatgtct tgcagtaggc gggctgatcg gcgcatggca aggtttttgg
    19021 gtagcttatg cagggatacc ggcgtttatc gtgactttag caggattgct gatgtttcgt
    19081 ggactgacac aagttgttct aggaggccaa tctctagcac cttttccagc tgttttccaa
    19141 aaaatgtcta cgggttattt accagactta agcgaaggaa gcagccttca cttcttgaca
    19201 ttgattttag gaatgatcat cacgatcgtg ttagttgttg gtcaatggcg aacaagaaat
    19261 cgtcggaaaa atcatttatt tgaagtggaa tctccaacag ctttcttggt aaaggcggta
    19321 tttacagcag tcgttgtact aggaataact tatatctttg cctcttatca agggtatccg
    19381 attattttga tcattcttgg tttgatcgtt gcagcatatg ggtttttgac aaataaaaca
    19441 gtagcaggtc gacagattta tgcgacgggc ggaaatcgaa aagcggctga actatcaggg
    19501 atcaaaacaa agcgaatcac gttctgggta ttcgtcaaca tgggagtcat ggcagcttta
    19561 gctggattag ttttagctgc tcgattgaat gcggcaacac ctcaagcagg tacatcgatg
    19621 gaactggatg caatggcggc agtatatttt ggtggtgctt cgacttccgg cggcattgga
    19681 acaatcatgg gtgcgattgt cggtggcttg gtcatgggtg tattgaacaa tggcatgtcg
    19741 attttaggcg tcggggtaga ctggcagcag gcaatcaaag gattgatctt gcttttagcc
    19801 gttgtgctag acttgtataa taagaaaaaa cgaacaacgt aggagaacgc cggttattca
    19861 gaaaaaagac tgtgacaaga catttgtcac agtctttttt tcgctaaaag cgaacattca
    19921 ttgtttcgtt tgtatagact aaaaataata tatgtttttt taccattcgt ataaattctc
    19981 tttttgctct tgattttttt gcttaattga gttaaattac atagggcgct aaaaaaagcc
    20041 ttgattaaat taataatgtt cgtgttttgc tggatgtgaa catcaggagg ataatgtaat
    20101 gaaagtattt gattacgaag atattcaatt aattcctaat aaatgtatcg taaatagccg
    20161 ttctgaatgt gatacaacgg tcacattagg taaacataca ttcaaaatgc cagtagtgcc
    20221 tgccaacatg cagacgatta ttgatgaaac aatcgcagaa ttcttagcgg aaaatggtta
    20281 tttctatatc atgcatcggt ttgatgaagc agctcgaatt ccgtttatca aaaaaatgaa
    20341 aaagcgtgga ttgattacct cgatcagtgt tggcgtcaag aaagaggaat attcctttat
    20401 agagaagttg gcagaagaat cgctaaatcc tgattatatc actatcgaca tcgcgcatgg
    20461 acatgctaac tcagttattg atatgattca gcatattaaa aaatatttgc ctgaaacttt
    20521 tgtgattgcc ggtaatgttg gtacaccgga agccgtccgt gaattggaaa atgcgggcgc
    20581 agatgcaaca aaagtcggga ttggtccagg aaaagtatgt attacaaaaa tcaagacagg
    20641 ctttgggact ggaggctggc aactggcagc tcttagatgg tgtgcgaaag cagctcgcaa
    20701 gccgatcatc gctgacggtg ggatcagaac acatggcgat attgcaaaat ctgtccgttt
    20761 tggtgcgaca atggtcatga ttggttcatt gtttgccggt cacgaggaat ctcctggcga
    20821 aacaaaagtc gaaaatggaa tcgtgtataa agaatacttt ggttctgctt cagaattcca
    20881 aaaaggcgaa aaacgaaatg ttgaaggaaa gaaaatctgg atccaacaca aaggatcttt
    20941 gaaagataca ctagtggaaa tgcaacaaga ccttcaatca tctatttctt atgctggagg
    21001 aagagatctg gaagccattc gaaaagttga ctatgtcatc gtgaaaaatt cgatttttaa
    21061 tggagacaca atctaaaaaa taaataaaat attaggatgt tgtgaaaaag ttgtcctgca
    21121 ttaagtaata agaacagcca aacaaaaata gttctttata tgttttgttt ggctggaatt
    21181 attatcgcag atacaagctt ttgaacatca ttattcagaa taagggactg tgataagatt
    21241 tttgtcacag tcccttatgt tttgcaaaaa aagtttttct tttttctcgt aaattcggta
    21301 aaatcaaaag aaaaaataga caatagaaaa gagaagctta tgtgggaaaa agtagagaaa
    21361 aaacaaggac cgatttatcg tcaaattatg gaacagatta tgagagaaat cgaaaacgga
    21421 aggatcaatc ctggaagtca acttgaatca gaacgaaaac tagggcaact ttttggtgtg
    21481 aatcgttcca ccgttgtcca tgcattggaa gagcttaggg agcttggggt actaacgagc
    21541 aaacgtggca gcggatggtt tgttaatcag actgaatggg ggaaattcgc tgttccaaga
    21601 atcgattggc ggcaaatgat ctctcctcgt tatgagcaga cggactggta tgagcaaaaa
    21661 ataagagaag caaagcagat ccaaaaaaca tcgtttcttg atttatacgc tagcgagatg
    21721 ccagatgagc ttttgccgaa ttttgagttt ccttcttatt ctcttgaaga aatcttagcg
    21781 gaagaaaaag aaatggatct tttaggctat actccgttac gtaacaaaat caaacggtat
    21841 ttggaaagta aatttgaatt ggcattttcc cccaaccagc ttcttgtgac tggtggcggt
    21901 cagcaagcaa tctttttgat tttacaaacg attctttcag ctggagaggg gatagctgta
    21961 gagtcacctt ctttttttta ccgattacct cttttcaaag cttcaggaac gcgtttattt
    22021 gggataccga tggatgaaga agggatcgac ttagaaaaac tgaaagaatc gatccataaa
    22081 aataaattga aagcagtgct tatcaacccg aattttcaaa accctacagg aaaagtgatg
    22141 agtcaaaaac gacgagaaaa gctagtagaa ttgtgtcgac agtatcggct tccaattatt
    22201 gaagacgatg tatttacgga tctttcattt ctaaaccaag aagaagtcct tcctatacga
    22261 acgattgatc cggataatgt gttatacgtc ggttcgttat ctagggtttt aggaaaaaca
    22321 acgaaaatcg gttgggtgat aggacctgag acttttatct cgcaactagc tcaagcgcaa
    22381 gaaatgatgg agttttcgat gaatatcctt acacaaatca cagtggctaa tgtatttaat
    22441 gaagcaattg atttgaaaat gcaagatgta agaaaaaagt tgaaagaaaa aagccgactg
    22501 ataagcgctt ggggagattc acaatcgttt ttcaagcttc attcaatttt aggtggttac
    22561 tatgcctgga ttacttggga tgggaaaaaa ctagaccccg aactagcaga aaaagtagta
    22621 gatatgggtc ttggaattgc accaagtcta ctttttgggc aagatataaa tggaatcaga
    22681 atcaattaca gtaggctgga tgaaaaccaa ataccgttgt tttattcaaa gatgaaaatt
    22741 ctagaaaagt ggttaacaga ataatttcac aaagtaaata gattaaagaa tataacagct
    22801 tggatttaaa tttcaagctg tttctttttt ttcggaaaaa gaatacgata taaagaaact
    22861 aagaaaaaac cattatttgt acgtccgttc acgatgtgta aataaaataa atgtttgcaa
    22921 caaaactgta taacaccaac gtttttaaat agaaacagat ggttgtaagt ttgattagaa
    22981 aatagaaaaa atgcagatga aatataaaac gctttcctta ttgtgatttt attcactaat
    23041 gctgttaata tgcggattgg cataatttcc tttcatcgtg aaaatttgtg cttttagctt
    23101 gtatttctca taaatccatg ataatttatt aatgaagacg gttaaatagt gatacagttt
    23161 tattcactgt ttcaatcgca gaatgaagag attttcttat agatgagaga ggaatgtgtg
    23221 acaaatggca aacaaaaaag caccgatcga cttccaagca ctgctagatg aagtcaatgc
    23281 agatttccca gtatatcaag cacttgatca agatggaaac gtagttaaca aagatttagt
    23341 acctgattta tccgatgatg aactcgttga attaatgact catatggtat ggtctcgtgt
    23401 attagaccaa cgttcaacag cgctgaaccg ccaaggacgc ctaggctttt ttgcaccaac
    23461 tgctggacaa gaggcaagtc agctggctag tgcttacgca tttgataaag aagatgttct
    23521 tcttccaggt tatcgggatg tacctcaact agtaaaacat ggtttacctt tatctcaagc
    23581 ttttttatgg tctcgaggac atgctgcagg taattcttat ccggaagaat tgaaagctct
    23641 accaccacaa attatcatcg gtgcacaata tgtacaagca gccggtgttg cattaggttt
    23701 gaagaaacga aacaagaaaa atgtcgtctt cacttatact ggtgatggcg gttcatcaca
    23761 aggtgatttt tacgaagcaa tcaatttcgc tggcgcttac catgcaaatg ccgtatttta
    23821 tatccaaaac aacgggtatg cgatctctac acctcgtgaa aaacaaactg cagcaaaaac
    23881 acttgcacaa aaagctgtcg cagctgggat tcctggtatt caagtcgatg gaatggatcc
    23941 gcttgcagta tatactgtat ctaaaatggc acgggaatgg gcgatcagtg gaaatggacc
    24001 agtcctaatc gaaacactga cttatcgtta tggaccacat acactatctg gtgacgatcc
    24061 aacccgctac cgtacaaaag acacagatga cgattggcag aaaaaagatc cattgatccg
    24121 cttccgtaaa tatttgacgg aaaaaggact ttggtctgaa gaaaaagaag aacaagtcat
    24181 cgaagcaact aaagaagaaa tcaaagcagc tattgctgaa gccgacaaag taccaaaaca
    24241 aaaggtttca gatttcttga aaaacatgtt cgaagtatct ccacaatcaa tcaaagaaca
    24301 aattgcaatt tatgaagcga aggagtcgaa ataatcatgg cacaaaaaac aatgatccaa
    24361 gcaatcacag atgccttagc acttgaacta gaaaatgacg agaatgtcgt tgtttttggt
    24421 gaagatgttg ggaaaaacgg aggagttttc cgggcaactg aaggattgca ggaaaagttc
    24481 ggagaagacc gagtattcga tactccttta gctgaatctg gaatcgcagg attgagtttc
    24541 ggtcttgcgt tagaaggatt tcgtccagtt cctgaaatcc aattctttgg ttttattttc
    24601 gaagcaatgg acgaagtagt agctcaaatg gctcgtacaa gatatcgaat gagcggcact
    24661 agaaatctac ccatcacaat tcgttcgcca tttggtggcg gtgttcatac accagaattg
    24721 cattcggata atttagaagg tttgatcgct cagtctcctg gtattcgagt agtgattcct
    24781 tccaaccctt atgatgcaaa aggactattg atttcttcta ttagaagtaa cgatccggtt
    24841 gttttcctag aacacatgaa actatatcgc tcattccgag aagaagtgcc ggatgaagct
    24901 tacgaagttc ctttggataa agcagcagtt actcgtgagg gaacagatgt atctattatc
    24961 acatacggag caatggtgcg tgaagcaatc aaagcagcag acaatcttgc aaaagaaaat
    25021 atatctgttg aaatcattga tcttcgtaca gttgcgccat tagatgtaga aacgatcatt
    25081 caatccgtgg aaaaaactgg tcgcgtagtt gttgtccaag aagcacaacg acaagcgggc
    25141 gttgccgcgc aagtagtttc tgagatctca gaacgcgcta tcctttcatt agaagcccca
    25201 atcggacgag tttctgcacc tgatacagtc ttcccatttg gacaagcaga aaatgtctgg
    25261 ttgccaaatg caaaagatat cgaagacaaa gtaaaagaaa tcgcggaatt ttaagaaaag
    25321 aagggacgaa acaaagtcat ggcttatcaa tttaaattgc cggatatcgg tgaaggaatc
    25381 gcagaaggtg aaatcgtcaa atggttcgtc aaaccaggag atacgatcaa tgaagatgat
    25441 acattactag aagttcaaaa tgacaaatca gtagaagaaa tcccgtcacc tgttacagga
    25501 acggttaaaa atgtgattgt tccagaaggt accgtagcaa atgttgggga tgtattagta
    25561 gaaatcgatg caccaggaca cgaagacaat gaaggggaca gtggtgtagc cgcagaatcc
    25621 caaactccag ctaaaccggc tgctgaaccc actgttgata cagaatcagc tggatcgtca
    25681 agcgaaggag tcttccaatt taagttaccg gatatcggtg aaggaatcgc agaaggtgaa
    25741 atcgtcaaat ggttcgtcaa accaggagat acgatcaatg aagatgatac attactagaa
    25801 gttcaaaatg acaaatcagt agaagaaatt ccgtcacctg tcacagggac agttaaaaac
    25861 gtgattgttc cagaaggcac tgtagcaaat gttggggatg tattagtaga aatcgatgct
    25921 cctggacaca atagtgcacc gtcaactagt gcgccatcag ctgaagcacc aaaagaaaaa
    25981 gtggaaacat caggttcagc aagtgtcgtt gaagctgccg atccaaataa acgtgtattg
    26041 gctatgccat ctgttcgtca atttgcccgt gaaaaagatg tagatatctc acaagtaact
    26101 gcaactggaa aaggtggcag agtaacaaaa gaggacatcg aaaacttctt ggcaggtggt
    26161 ccaagcagtg caccagcgaa atcagaagcg ccagaagcgg cagcaccgaa agaagcagct
    26221 cctgcagcag aaagcaaacc agctgcacca gcaaaaccat tcaagtccaa cttaggagac
    26281 ttggaagaac gtgtagcaat gacgccaaca cgtaaagcta tcgctaaagc aatggtcaac
    26341 agtaagcata cagctccaca cgtgacattg catgatgaag tagaagtgtc taaactatgg
    26401 gataatcgga aacgatttaa agaagttgct gctgcaaatg gtacgaaact tactttcttg
    26461 ccatatgtcg tgaaagcttt aacagcgact gttaagaaat acccagtatt gaatgcatct
    26521 atcgacgatg caaaccaaga aatcgtttat aaacattact acaatatcgg tattgcaact
    26581 gatacggatc acggactata tgtaccaaat gtgaaagatg cagaccgcaa aggaatgttt
    26641 gctattgcag acgaaatcaa tgagaaagca aaattagctc atgacggcaa actatctgca
    26701 gaagatatgc gcaatggaac aattacgatc agcaatatcg gatctgtagg tggcggttgg
    26761 tttacaccag tcatcaacta tccagaagta gctattttag gtgtaggaac gattgcacaa
    26821 caaccaatcg taaatgctga aggcgaaatc gttgtcggtc gtgtgatgaa actgtcatta
    26881 agtttcgatc accgtatcgt agatggagca actgcgcaac aagcaatgaa taatatcaaa
    26941 cgtcttttag ctgatccaga gctattaatg atggaaggat gataaacaaa tggtagttgg
    27001 agattttgct gtagaattag atacagttgt aattggtgcg ggacctggtg gctatgtagc
    27061 tgccatccgc gctgctgaaa tgggacaaaa agtagcaatc attgaaagag agtatattgg
    27121 cggtgtttgt ttgaatgtcg gctgtattcc ttcaaaagca ctgatagctg caggacacca
    27181 ttatcaagag tcactagatt ctgaaatgtt tggagtaact agtgaaaacg tcaaactaga
    27241 ttttgcaaaa acacaagaat ggaaagaaaa caaagttgtc catacgttga cttctggtgt
    27301 tggtttccta ttaaaaaaac ataaggttga aacaatcgaa ggagaagcgt tcttcgtaga
    27361 tgatcacaca ctacgcgtca ttcatccaga ttcagctcaa acttattcgt tcaataacgc
    27421 aatcatcgca acaggttccc gtccaatcga gattccagga tttaaattcg gaggacgtgt
    27481 gttagattct actggcggat tagcattgaa agaagtacct aagaaatttg tgatcatcgg
    27541 tggcggtgtg attggtgccg aattgggcgg agcgtacgca aacttaggag cagaagtaac
    27601 gattcttgaa ggctcaccac aaattttacc aacttacgaa aaagatctag taaaattagt
    27661 agaagatgat ttcaagaaaa aaggcgtaac agtcgtaacg aatgcaatgg cgaaagaagc
    27721 agtcgataac ggtgacagcg tgacagtgaa atatgcagtg gacgggaaag aagaatccgt
    27781 tactgctgat tatgtgatgg tcactgtagg tcgtcgtccg aatacagatg atatgggctt
    27841 agaacaagcg ggtgtcgaag taggagaacg aggattgatc acggtggata aacaaggacg
    27901 tacgaacgta cctaacatct atgccatcgg tgatatcgtt ccaggggcgg cattagcaca
    27961 caaagccagt tatgaagcaa aaatcgctgc tgaagcaatc tctggcaaaa aagtagctgt
    28021 agattataaa gcaatgccgg cagtagcctt cactgatcct gaattggctt ctgtaggtat
    28081 gacgatcaaa gaagcaaaag atgctggtat cgaagcaaaa gcatataaat tcccattttc
    28141 agggaacggt cgggcattat cgcttggtaa aacagaagga ttcatccgtc tagttacgac
    28201 gatcgaagat aacgtgctta ttggcgcgca aatcggtgga gtcggcgcaa gtgatatggt
    28261 ctctgaatta gctttagcaa tcgaatcagg aatgaatgct gaagatatcg ccttgacgat
    28321 tcatcctcat ccatctcttg gcgaaatcgt catggatgct tcggaattag cattaggttt
    28381 accaatccat atttgatttg aataatacag gcgaaacgca actgaaaaat cagttgcgtt
    28441 ttgtttttta gaattatcaa taatattatt atgtaaactt aaaaatatga caacttcttg
    28501 gtttattacc acttcctatg ctacactgaa gaggaatatg aaataggaga gttgaagatg
    28561 caagaatatc agtatccact tgatttagat tggacaacag acgaaatggt cattgtaatg
    28621 aagatgtggg aagatctgga acgtgcgaat gaaaaaggga taaacaatga agcttttttg
    28681 aagacttatc agcagtttaa aactgttgtc aaatccattg gtgaggaaaa acgattggga
    28741 cgggaatttg aaaaagcttc cggttattct ttatatcgta cagtgcagca agcaaaaaaa
    28801 aatccaaaca aaactttgag aatgaacggg tgagataaat ggaacagatg attgatcata
    28861 tcaagtcctg gcttgaagaa gctggtgaga aaatcaaatc ttctcaacaa acattaacaa
    28921 tctcgcaaaa atcaaatcga acggatctag taacaaatgt cgatcgaaac atccagaatt
    28981 ttctaatcga aaaaatccat tcctttgatc cagaggcgaa aatattggca gaagaagaag
    29041 ggtgtaatca attatcggat ctcgatggaa gagtctttat catcgatcct attgatggca
    29101 ccttgaattt tgtgattgaa ggggaaaatt tttgtattat gctcgctgtt tatgaatccg
    29161 gcgttggaaa gcttggtttc atttatgacg tgatgagaaa tgaacttttt tggggtggaa
    29221 aaggaatagg cgtgtataaa aatgatgagc cattgcctag accagcaaat aaatccttat
    29281 cggaaggctt acttggtgtg aatagttatc ttttcggtca taataaatat ggaatccgag
    29341 aaattgcgga aagcagcatg gggacacgta tgtacggatg cgctggtctt gaactagtgg
    29401 caatcttgaa aggaaaccac attgcttata tttcaaacct tagcccttgg gattatgcag
    29461 cgggaaatgt cttattggaa gagtttggta tggtaacaag cggtcttttt ggtactcctt
    29521 tgacatttga aaaaagggaa tactatttag cagggacttc ttgtgcatat gcagaaatcg
    29581 cagacctttt gaaaaaagaa ggctgaaaaa aaggtagaaa tcattcacaa aaatgataaa
    29641 aacgagcaaa agattaattt tctcgaaaaa atgaaaacat tttactatat ttcatgaaaa
    29701 aagtctgcta taattagcag tcgagtaaaa tttgccctgt cataaagaac caataaaaaa
    29761 ggagttagtt acattgaatt atagaaacga tatccgtaac gtcgcaatta ttgcccacgt
    29821 tgaccacgga aaaacaacat tagttgatga actattaaaa caatcagata cattagacgc
    29881 acatacgcaa ttgcaagaac gtgcgatgga ctcaaatgcg ctagaaaaag aacgtggtat
    29941 cacgatttta gccaaaaaca cagctgtaga ctataaaggt attcgcgtaa acatcatgga
    30001 tactccagga cacgcggact tcggtggcga agtagaacgg atcatgaaaa tggttgacgg
    30061 cgttgtgttg gtcgtagatg cgtatgaagg aacaatgcct caaacacgtt ttgtattgaa
    30121 aaaagcactg gaacaacata tcacaccaat cgttgtcgta aacaagattg acaaaccttc
    30181 tgctcgtcct gagcatgtcg tagatgaagt tcttgaacta ttcatcgaat taggagcaga
    30241 tgatgatcag ttagactttc ctgtgattta tgcatctgca ctaaatggta cgtcaagctt
    30301 gtcagatgat ccagcagatc aagaaccaac catggcacct atttttgata caattattga
    30361 aaaaatccct gctcctgtcg ataacagcga cgaaccatta caattccaag tttcattact
    30421 tgactacaac gattatgtag gtcgtatcgg aatcggacgt gtatttagag gaacaatcaa
    30481 agtaggagac caagtagcat taattaaatt agacggaaca gttaagaaat tccgtgtaac
    30541 gaaattattt ggtttctttg gactaaaacg tctagagatt caagaagcaa aagctggaga
    30601 cttgattgct gtctcaggta tggaagatat ctttgtcggt gaaacagtta ctcctgtgga
    30661 tcatcaagat gcgttaccta tccttcacat tgacgaacca acattgcaaa tgactttctt
    30721 agttaataat tcaccatttg ccggtcgaga aggtaaattc gtaaccgctc gtaagattga
    30781 agaacgtttg atggcagaac ttcaaacaga tgtatcctta cgagttgaac caacaaactc
    30841 tccagatgca tggacagttt ctggtcgtgg agaattgcat ttgtctatct tgattgaaaa
    30901 catgcgccgt gagggatacg aattgcaagt atctcgtcca gaagttattg aaaaagaaat
    30961 cgacggtgta aaatgcgaac cgtttgaacg cgtgcaaatc gatacaccag aagaatatat
    31021 gggtagtgtt atcgagtcct tgagcttacg taaaggtgaa atgcaagata tggtccatac
    31081 aggaaacgga caaatccgtt tgacattctt aacacctgcc cgcggtttga ttggttattc
    31141 aactgaattc ctttcaatga cacgtggtta cggaatcatg aaccatactt ttgaccaata
    31201 tctgccaatg cttcctggtc aaattggcgg acgtcaccaa ggggcgcttg tttcgatcga
    31261 tacaggaaaa gcaacgactt attctatcat gagtatcgaa gaacgcggta cagtcttcgt
    31321 agaaccaggt acagaagtat atgaaggaat gattatcggg gaaaacagcc gtgataatga
    31381 tttgactgtc aacatcacga aagcaaaaca aatgacaaac gtacggtctg ctactaaaga
    31441 ccaaacatct gtcatcaaaa aaccaaaaca attgacgttg gaagaatctt tggaattctt
    31501 gaatgatgat gaatattgcg aagtaacacc agaaagcatt cgcttgagaa aacaaatttt
    31561 agagaaaaat gctcgtgaaa aagcatctaa aaagaaaaaa taatctgtaa aaggatgaag
    31621 agagaaacca gcttgttata aaaaatagct ggtttctttt tttgtccaac tcttttctgt
    31681 ggagaagtca tctttgtcaa tggacataaa ttcagaaaat acgccgaaag acgtttaaaa
    31741 gacttttaaa aaaacttatt tctagagagt ttttgtttca taataagata atgtttcacg
    31801 aaaggagatc aacttgtcct tcgaagaaaa tcgaagtaaa atggcaagag aaaggagcgg
    31861 gaagatgaac gacttattaa aacaatactt tggctatgat gagtttagac ctggtcaaaa
    31921 agaaattatc cagaaagtca ttgatcagga aaatgtatta ggaatcatgc ctaccggaag
    31981 tgggaaatca atctgttacc agcttccagc tttactatta gatgggctta ctgtggttgt
    32041 gtccccttta atctctttga tgaaggatca ggtggacgcg gcgaatcaat taggtatacc
    32101 cgccactttt atcaatagct cgttagatgg ttacgaaaca gctagacgct tccaagaaat
    32161 cgatagacag cagtaccgtt tactttatat cgctcctgaa cgttttatca tgccggattt
    32221 tatccaagca atgaagcgat ggaacgtccg tatgatcgct attgacgagg cacattgtat
    32281 ctctcagtgg gggcatgatt tccgcccaag ttatttacag atggcgaacc agctagatca
    32341 actgccaaat cgtcctgtaa ttgttgcttt gacagctaca gcgactgtgc aagtagctgc
    32401 tgacataaaa agacttttaa aaattccaga aaacaaccat attcagactg gttttgaacg
    32461 agaaaatcta cgatttcaag taattaaaga tcaaaaaaaa gaacaatatc taatcgaata
    32521 tttaaagatc aacaaaaatc aatctggtat catttatgca gcaacgagaa aagaagtaga
    32581 ccgtctctat catttgctta aaaaattcga tttttccgtg ggtagatatc atggtggtct
    32641 aaatgagaat gagcgaacag aaatgcaaga agccttttta tacgatcgct tgcagctgat
    32701 tgtagcgaca aatgcatttg gtatgggtat aaataaaagc aatgtccgtt ttgttatcca
    32761 ttatcaaatt cctggctctc tagaagcata ctatcaggaa gctggtcggg cgggaagaga
    32821 tggtctttcc agtgaagcca tcctgctttt ctctcctcaa gatatccaag tccaaaagtt
    32881 ttttatccag cagtcccaaa gagaagaagg gcaaaagcaa aaagaatatg aaaaattaaa
    32941 agcgatgaca gaatacgtgt atattgagtc ctgtcttcag caatacatcc taaactattt
    33001 tggcgagaca agcagtccgt gcaaccgttg tggtaactgt ttagatgaca gagaaatagt
    33061 agaagtaacg acagaagcac agatggttct ttcttgctta aaacgtatgg gtgagaacta
    33121 tggtaaacaa atgctgatga aagtcttagc aggatcgaaa gaacaaaagc tgcaagcttt
    33181 aggttttgga catttatcta cctatggact gatgaaaaac caatcgcaaa aagaaacgat
    33241 gcagttaatt gaatatttga tatcgaatgg ctatcttctg accatcaatg gtgagtatcc
    33301 tgttttgaaa gtaacagaaa gagggataca agtattgaaa ggacaagaat ctgtttatcg
    33361 aaaggaaccc aagaaagtcc agcagctatc ggatgaagaa accgatacac tatttgaagt
    33421 gctgcgggaa ctgcgaacgg atctagcatc agaagcaggc gttcccccgt atgtcgtatt
    33481 ttcagattcc acattgaaag aaatgagccg aatacgtcca tcaagcagat tggagatgct
    33541 tcagataaaa ggagttggcc aaagcaagct tgataaatat ggcgaagcct ttttatctcg
    33601 gataaaaaat gcggactcta acgttctgga taagtagaaa gaacgaatac gtaaaaatga
    33661 ataaaaacaa ctggttaaat ctgttttttt tgctccttat gaaaatcaaa tatgttataa
    33721 ttaaggaatt agaatgggag gtaagcacag atggattctt tgtcgaaaaa acttgcgatc
    33781 caagtattaa atgatgaagc agaacggatc aaacgattaa tccgaaatca aaacaacagt
    33841 ctctgtatct cacaatgcaa agccttcgaa gaagtggtag atacacagat gtacggcttt
    33901 tcccggcaag tcggttttgc tgtccgtata ggagtaatcg gaaagaacga gggccagctg
    33961 atgttaagtg agctagaacg agaattgaat cagttatata gtgaggttta ccaagaaatt
    34021 tatgataaaa aagaagttgg caaggaggaa tagacttgcc taagaaagtg aaaaaaaggc
    34081 atctattaga ctatagcatc ttgattccct atttaatttt atgtgtattg ggattgatca
    34141 tggtatatag ttctacgtcg tatctattat tagaaaacgg acaaaatccg tccgcatcag
    34201 tcattaacca gagtattttt tgggtgctta gcttgattgc tatcgcacta ctttataaaa
    34261 tgaaaacaga tgttttgaaa aaccagcgtt tgatcatggc agccatcgca gttttgacga
    34321 ttttgctgct gattgttgtg ttttttggta aggagatcaa tggggcgaaa ggttggctgc
    34381 aaattgcagg attctcgatc caacctgccg aatacctaaa aatcatttct atctggtacc
    34441 tgtctctgac tttatctaaa agacaaaaca gtgtacaaaa agattttctt gggactgtga
    34501 aacgtccact tgcaatggtt atcggactta ctgcattagt cgctattctt cccgattttg
    34561 gtaatgcggc agttatcttt ttgattattt tggttctgct tttagccagc ggagtcaatt
    34621 atgtgtatac tttgattgta ggagtaggag gcttttgtct aagtactttt acgatctggc
    34681 tgatcaatat cacaaacgga aagatcttac ccggacgttt acagtatatc tataatcgat
    34741 ttgctattta ccaaaatcct ttctcagacg aattaaataa agggcatcaa ctagtcaacg
    34801 gctactatgc catgttcaac ggaggactat ttggcagggg attaggtaac agtatccaga
    34861 aaaaaggatt tttacaagaa gcacaaaccg attttattta tgcaatcgta gtggaagaac
    34921 tgggagtgat tatggggatt ttgatattag cgttgctatt ctttatgatt gtgcgcattg
    34981 tccttgtagg tatccgttca aaagatccgt tcaattcgct tttgtgcatt gggatcggcg
    35041 caatgttttt gatccaagta tttgtcaatc ttggaggaat tactggcgtt attcctttga
    35101 cagggatcac gttcccattt ttaagtcaag gaggctccag tttgctgatg ctttcgattt
    35161 gtgtcggctt tgtcctcaac atcagtgcgg atgaaaaacg caagagctta ggtttataat
    35221 aaaaaaagat gtaggagtgg ttcgatgaaa aaagttttag ttgctaacag gggtgaaatc
    35281 gctgtacgtg tatttcgtgc ttgtacagaa ttaggaatta aaactgttgg aatctatgca
    35341 gaagaagatg aatattccgt acaccgtttc aaagcggatg aagcatattt agtaggacaa
    35401 ggaaaaaaac cgattgatgc gtatctagat attgaaggga tcatttctat cgcgaaagaa
    35461 tgcggagctg acgctattca tccgggttac ggtcttttgt cggaaaactt gaattttgcg
    35521 caacggtgcg aagaagaagg catcattttt gttggaccca aactgcatca tttagatatt
    35581 tttggagata aaatcaaagc aaaagctgca gctatcgaag cggggattgc ttcgattcca
    35641 ggcacagatg gaccaatcgc ttctatagac gatgccttgg agtttgcgaa acaatacggt
    35701 tatccgatta tgatcaaggc agctctaggc ggcggcggac gcggcatgcg tgtagctcac
    35761 gatgaaaaga gtgcaagaga gggctacgaa cgtgcgaaaa gcgaagcaaa agcagccttt
    35821 ggtagtgatg aagtgtatgt tgaaaaatac atcgctaatc ctaagcatat cgaagtacag
    35881 attctcgggg atacgcatgg aaacgtcatc catctttttg aaagagattg ttctgttcag
    35941 cgccgtcacc aaaaagtagt agaagtagcg ccttgtgtct ctatgaatga acagcaacgt
    36001 caaaaaatct gccaggcagc tgtccaattg atgaaatatg tcgggtatgt caacgcaggt
    36061 acagtagaat ttcttgttga aggagatgat ttttacttta tcgaagtcaa ccctcgcgtc
    36121 caagtggaac atacgatcac agaaatgatt acagatattg atatcgtaac aacgcaactg
    36181 ttgattgctc aaggtttgga tctgcataaa gaaatcggat tgcctcagca agaaggaatt
    36241 aaattaaatg gttcagccat tcaatgccga atcactacag aagacccgtt gaataacttt
    36301 ttaccagaca cagggaaaat cgatacgtat cgttcacctg gtggttttgg cgtacgtctg
    36361 gatgtcggaa atgcttacgc gggctatgta gtgacaccgt attttgattc attgctagta
    36421 aaagtatgta cgcatggtgc tacctttgaa acagccattc agaaaatgga acgttgcttg
    36481 aaagaattta gaatccgtgg agtgaaaaca aacattccat ttatgctcaa tgtcatcaca
    36541 catcctgaat tccaatcagg aaatgcaaaa acaaccttca ttgatagtac agcaacatta
    36601 tttgaatttc ctcgactacg agaccgcgga aacaaaacga tgaaatatat cggtgaaatt
    36661 actgtgaatg gttttccagg tatcgaaagt ggcgaaaaac cattttatga agaaccacgt
    36721 atgcctaaag atctaatcac aagaactgat tacgtaacgg ctaagaatgt attagatgcg
    36781 aagggtgccg atgctttggt cgaatggatc aaaggacagg aaaatttgtt gttgacggat
    36841 acgacattta gagacgccca tcaaagtctg ctagcaactc gtgttcggac aaaggatttc
    36901 aagcagattg cacgtttgac tggagaaggt cttcctgaac tcttttcaag tgagatgtgg
    36961 gggggagcaa catttgatgt ggcttaccgc tttttgaatg aagatccttg gcaacggttg
    37021 agaaaaattc gttctttaat gcctaatacg ttgctgcaaa tgcttttcag aggttcaaat
    37081 gccgttgggt attctaatta tcctgacaat gtattggtgg aatttgtcaa agaagcagca
    37141 gcgcaaggaa tcgatgtatt tcgaatcttt gatagtttga actggactcc acaaatggaa
    37201 aaaagtatcc aagctgtacg ggatactggt aaaatcgcag aggcagctat ttgctatact
    37261 ggcgatatca acgatccaag tcgggcaaaa tacaatgttc agtattataa agacatggca
    37321 aaggaactag aacaactggg tgcgcatatc attgcaatca aagatatggc tgggctgctt
    37381 aaaccgcagg cagcttatcg tttgattagt gaattgaaag aaacgacaga tctgccaatc
    37441 catctgcata ctcatgatac cagcgggaat ggaatcatca cttattctgc tgcttcaaaa
    37501 gcaggagtag acatcgttga cgtggcaatg agtgcaatga gtgggaatac tagtcagcct
    37561 agcatgagca gcttgtatta tgcactggtc aatggtccac ggctgccaga aatcacgatt
    37621 gaaaatgcac agaaattaaa ccattattgg gaagatgtgc gcatgtatta caaaccgttt
    37681 gaaaacggct tgaatgctcc tgaaacagaa gtatatatgc atgaaatgcc aggaggacag
    37741 tattcgaatc tacagcaaca ggcaaaagct gtcggattag gtcatcgctg ggatgaaatc
    37801 aaacaaatgt atcatactgt caacttgatg tttggcgata tcgttaaagt aactccatca
    37861 tctaaagtag ttggagatat ggcactattt atggtccaaa atgatcttac agaagaagat
    37921 atttatgaaa aaggcgaaac actaagtttc cctgaatctg ttgtgacttt cttccaagga
    37981 gaactagggc aacccgttgg cggatttcct aaaaaattgc aaaagattat tcttaaaggc
    38041 agacctgcat tgaatgaacg tccaggtctt ttggctgaat cagtcgactt caacgaagta
    38101 aaaaaagaat tggcagaaaa aattggctat gaaccaaaac aagacgaagt attaagctat
    38161 ttgatgtatc cgcaagtctt tttggactat caaaaagcat ataatcaatt tggcgatgtt
    38221 acattactgg acacaccaac atttttccaa ggtatacgtt tgggtgaaac aatcaatgta
    38281 caaatcgaac gcggaaaaac gcttatcatt cgtttagatg aaatcggaga acccgatatc
    38341 gaagggaatc gcgtactgtt cttcaatttg aatggacaaa gacgtgaaat cactatcaaa
    38401 gataattcga tcatcagtac ggtccaaacc cgattgaaag ctgaaccgac aaaccgagaa
    38461 caaattggag caacgatgtc tggttctgtt ttggatgtat tagtgaaaaa aggcgataac
    38521 gttaaaaaag gtgatacgtt gatgattact gaagcgatga aaatggaaac ggccatcgaa
    38581 gcacgttttg acggagaagt cgcacatgta tatgtgtctt caggagacac tatttcgtct
    38641 ggtgaccttt tgattgaagt gactgagaaa taataagcga aaggtgggga aacatgaaaa
    38701 gactgggttt gttttggctg attctgctat tagttattgt ggcattctac atggaaccag
    38761 tcttgtttcc gccaaaactt gaagtgaagg aaaactctga tcaacaaata tcaaaccaaa
    38821 tgacaacgtg gaaataccag gagcttaaag caactggttt ttctacttat atcggaaaac
    38881 cgatcgatca gctggaaaag gattttggcc aagcctatga tcgattaact agcggatttg
    38941 gttttgaaac aagatattat gaaaataaag ctagtcatct cacatttgaa gcgaacatcc
    39001 aagacagcag agtcacaact gttaaagtac tgcaaacagg gcctgctgat atcgcgccgt
    39061 tttatttagg gatgacgatg caagatctta cgaaaatcac tactatctat actaatttca
    39121 cttttgagta ccatgataca gaagttggaa tcgaactaat ggaagaggat atgaattatc
    39181 gtccgctaat tgcttttgat aacgacactt ttgctatttt attttttgat caaacaacaa
    39241 acggcctatt ttcagttgtg tatttagata aagatagtct tttgaaacta ttgccatatc
    39301 aagtgtttgg tgaaggcctt ccacattatc aagcagataa atcggctgat tgggaatcaa
    39361 tcaatcgcgt taaagaacga aaaagtacga ctttattaaa tatgcttaga agagaagacg
    39421 aacttcccat atacaatcaa acacttgcct ttcaaaacca cagtcagctg ctactacatg
    39481 attttttgac aaggcctgag gaaatattgt cagaggaacg tatggctgat tggcaaaaat
    39541 cgctaggcag tgctcaaacg acagtgaaat tctcactgac gaatgatgaa ttggagaagt
    39601 taatcagtaa acaaaaatta gaaaatacta gtgggctatt tattcatccg gcgattgacg
    39661 ctactttttc gtttttatat tggtacagcg atccttacaa ccatgaccgt ttcatggacc
    39721 cgggaacgga ttctgtaggc attgcttttt ctaaagaaaa tatgttagtc ttattgcaag
    39781 aagaaaacgg agaaagtagt gatagcagtg atcgttaacg aagagctgtt tcaactggag
    39841 gatcaatgcc tccaagtcgc tgaaatgata aaaaagagta aaacgatggc ttcttatctt
    39901 gaacataaac agtcaatgga agaatctgca aaagtttgcc agttgaaaga agcgttcttg
    39961 tctaagaaag aagcatttga aaatgtagcg gcatatggtg agtatgcacc tgattatcgt
    40021 gaaaaaaaac gagctgttcg aaaagcaaaa agacagcttg atatggatga gcatgttgca
    40081 gctttccgat atagtgaaac acagctgcaa agtctgttgg atcaaattgt ccaagtactt
    40141 gccgaagaag tttctaatga aatcaaagta gatgcaggta gcccgttttt tgaaacgaaa
    40201 aaacactttg gatgcggagg taattgtcat gcaattagac aatagtgaat ttgaaataac
    40261 aaaacgacgc gggctgatcg tatgggtcta cagcttaaaa caattaaaga atctgaaaaa
    40321 gttcggcttg ttgcattatg tctcaagaag aatgaaatat gtcgtgcttt acttaaatga
    40381 agagacattg gaagaaacag aagaacgaat ccgtaaatta cattttgttc gaaacgtaga
    40441 acgttcttac cgaccggata tcgaaatgaa ttttgccgaa aaaataggaa acaaaacagt
    40501 attaaaagac gaaggtttcg aaattgaaga gctaaataca gaaatccgat tagctgaaca
    40561 tctttaaaaa tctcttatga gcagttttta aataaaaaat aacttggata acgatcgatt
    40621 tttgatcatt gtccaagtat ttttttggta tactagtacc agtaccattt ctaaggagtg
    40681 agtagatgcg gataatatca ggaaattatg gcgggcgccg tttaaaaagt ttagcagggg
    40741 cgaacaccag accaactaca gataaagtaa aagaatccat attcaatatg attggaccat
    40801 attttgatgg agagacagta ttggatctgt ttgcaggaag cggtggttta gctatcgaag
    40861 cagtatctag aggctgcagt catgcggtat gcgtagacaa aaattaccaa gcgctaaaaa
    40921 tcatcaaaga aaacatcgaa attacgaaag aaccagagaa atttaccaca ataaaacttg
    40981 atgcggacaa agcaatcgaa cggctagcag cggagaaaca acaattcgac tacttgtttc
    41041 ttgatccgcc atatgcaaaa caaaaaataa tcgatcaaat ccataaaatg gaagaactgg
    41101 gacttttttc taaaaccgca gttatcgtat gcgaaacgga taaaacagtc gaattgccag
    41161 aaactgtggc tggattccat caaataagaa aacagattta tggcataacg gccgtaacta
    41221 tctatagaaa agagggaaat caatgaaacg tgcattattc ccaggaagtt ttgatccttt
    41281 tacaaaaggt cacttagata cggtggaacg tgcggctaaa ttattcgatg aggtagtaat
    41341 tggtgttttt atcaatactt caaagaaaag tttgtttccc ccagaagaaa gaatgacatt
    41401 gatcacaaaa gctgtttctc atcttcctaa tgtaaaagtc atgcatcaag aaaatcaatt
    41461 gacggtagaa acagcaaaag agattggtgc agatgcgttg atcagaggca tacgaagtat
    41521 caaagacttt gaatatgaac gagagattgc tcagatgaat catcatctat atgatgaatt
    41581 ggaaactgtc ttcttattag caaaaccaga atatagtcac gtgagttcca gtattttaaa
    41641 agaagtactg cactttggcg gagatgtgag cagctactta ccgccagtga tcaacgaagc
    41701 tttagctgga aagcgtgaat caaatgaaag ctaaacaatg gttgaaaaca gtactgatta
    41761 tcatatttgg aatgttgatc attggatttg tagtagttcc cttgccgtat tatgtagaat
    41821 caccgggagc cacgatcgat ctgaaagaac taattacggt caatgataag gaagataaag
    41881 gttctgggtc tttctctctt acctctgttg gtatcaggca agctacaggt ttcttagctt
    41941 taaaagctaa aatgacccct tttgaagaat tgatcagtga agaagaattg actggtgggg
    42001 cgacaaatga agaatataat caaatgcaac agtattatat ggattcttcc caaaatgcag
    42061 caatcgaaca agcgttaaaa cttgcagaca agccttataa aatggaattc aaaggcgtat
    42121 atgtcatgag tgtagagaaa acatcgaatt tctttggcaa gctttcagtt ggagatacag
    42181 taacaaaagt agacgggaaa agctttcaaa gtacagaagc atttatggat tatgtaaaaa
    42241 gccaaaaagt tggacaaaca atcgaaatca gttatttgca taatggtgaa gaaaaaacag
    42301 cttctggtga tctgatcgag ttaccaacag ataaaaaggc aggaataggt atcacgctta
    42361 ctgaccatac cgagatcgaa tcagatacag atgttcgtat taattctggc tctatcggtg
    42421 ggccatcagc gggattgatg ttcactttag aaatctatga acaagtcact ggtaaaaact
    42481 tgcgacatgg taagcaaatt gcaggaactg gaacgatcaa cagtgaagga gaagttggcc
    42541 gaatcggagg aatcgataaa aaagtggcaa gtgcggataa agctggcgta gagattttct
    42601 ttgcaccaga tgatacgatt tcagacgatg ttaagaaaga atatcctgaa ataaaaacca
    42661 actacgaaga agcaaaagct gccgctaaaa aattgaattc ctcaatgaaa atcgtaccag
    42721 taaaaacagt acaagaagcg cttgactatc tagagaaaat gaaatgaaac taaataatta
    42781 aataggggtt gtgacacgag ttctgtcaca acccctcttt ttcgtcaaaa atcttcacga
    42841 taaaaataaa aacaaaagaa aaaattgacg taattagatg ttgaagggac gattttgcca
    42901 gagctcactg tttcttcaat tttaaatgaa aggagtaatg aatgattttt actggtcatt
    42961 ctttgaaaaa agaacaatga tagaaaaaat cagcaagaaa atactagctt cacccaaatt
    43021 gatcatttgg atgtctatga tagttcttat cttttgtatc gcaagtggtt ttttattatc
    43081 aaatttgcta ttttatagag aaacagaaga gactgctgca aaagaaatac tttcttcgtt
    43141 tagttccagt ggagaagatc catcagctga aggaaacgat gtagaagagg atgctcgtcc
    43201 aaaggtcatg tacaccgata tcaaaggaag tgtgaaggag cctggtatct attccttttc
    43261 ttcagaagaa cgggtctatg atgtactaaa gcgtgcaggt ggattattag aagaagcgga
    43321 tagcgatcga atcaactttt ctgcgaagat agaagatcag caagtcctat atatccctgc
    43381 tgtaggtgaa gagctgccag aacatcttga tcagttggca tcctcggaag gaaaacagcc
    43441 tacagcagat tccgagcctc ccaaaataaa tatcaataca gccactccat cagaacttca
    43501 gcagattcct gggatcggat cagtcaaagc gcaagaaatc attcgattta gagaagaaaa
    43561 cggatccttc cagaaagtgg aagacctgca ggagatttct ggtatcggtg aaaagacagt
    43621 tgaaaaacta aaaaacttcg ttacaattaa atagaatgat tcaaaatcga ctggcacgag
    43681 caataaatgg acgctaatgg atactaagaa taatccaatt ctaagagctt ggcaggacga
    43741 ggaggtagta tttttggata ggaaatagct tgattgttcg caaagaaaca ttaaaaaaac
    43801 caatgctgaa tgtctatctc caaaataaaa tatctgggat tcatattatg aatacggctg
    43861 tcagcggcaa taacagtcaa gctctacgag aacgctttgc caaagatgta cttagctata
    43921 cagcagataa agtatttatt ctcattggga cgaatgatct agcagagcat aaacaacttt
    43981 ctaaagaaac gtaccaaaaa atctgcagtg gatgatagtt cagctgaaaa aagactatag
    44041 ttctgatcat gctttcttta tcacgccacc agcagtcgat gagacaaaac aaaaatatcg
    44101 gaataacaag ttgatcaaag aatatatctc tgttctcaca gaagtagcaa aacgccatga
    44161 ttgtgaagta tcgattttta tacggaattt cttgaacaaa tggattcgaa gaatttttat
    44221 aatgggctaa tggatgacgg acttcatttc ggcagtgaag ggtaggatct tttgtcagac
    44281 aggatgattc gttatattta aagaatattt tgtttaagca atgagtactt tttaatcaac
    44341 ttgaaaaaac ggaggaatat aaaacatgtt agaaaaatct tcaaaagcat gcgatacgaa
    44401 taaagaaaaa gttactgata agtcacataa tgaacgcatt ccttgggacc agtactttat
    44461 ggctcaggca gtactgttat ctttacgtag tacgtgtaca cgtttagaag taggagcgac
    44521 actcgttcga gaaaaaagga tcattgctgg cggctataat ggagcagtca gtggagatgt
    44581 ccactgcata gatgaagggt gttacgtcgt ggatggacac tgcttgcgaa cgatccatgc
    44641 agagatgaat gcactcctac agtgtgcaaa acttggtatc ccgacggaag gttcagaaat
    44701 ctatgtcaca cattttccat gtttggcctg tacaaaggct ttgttgcaag ctggtgtgaa
    44761 agggatcaat tatctccatg actatcgaaa tgatccttat gcccaagcgt tgatcgaaca
    44821 attaggtgta tctgtacacc aagttgcttt agactcacag catttttcaa aacttcaaaa
    44881 tgaattaaca ggaaaacatg tctagcgttt tagaaaggca tgttcgaaat cggttgattt
    44941 ttccggcaat tacgtcgatg attatctgtt tgttgtatta cactgacaat tggggttatc
    45001 gtttgcttct tgccggaatt tttttatggt tttcgctaaa agaaggacgt tttatcgcgt
    45061 acttgtgtat tttgtgtagc ataacggtat ccatttctta taggctacaa gagtattcat
    45121 ttttagagga aaaaacaaca tctgcgcaaa ttactgtatt tacagatacg attcgtgcaa
    45181 atggtaattt tttgacaatg gaaggaaggt tatcagagaa taaacaaaag ctccagcttt
    45241 cttatcaagt aaacacagaa gcagaattga aaacatggct taacttccga ggaaagggaa
    45301 tagaattgac tgctgaaggc gagttcatct ctccagcaaa aaagcggaat ctttataatt
    45361 ttgaccaaga agcttacttc aggaaccata gaatttctgg acggttccaa attcaaaaga
    45421 ttgaacagat caccatgcaa aatcactggc gaaactggct tcagagaaaa agaggatctt
    45481 ttatctcctt tgtccaatct gaatttccgc aaaaaacaag tgtatatatt aattctttat
    45541 tatggggcta taaagatgca ggattcagag aaccagaaga aatttttagg gagagtggtt
    45601 tgctgcatct gttcagtcta tcgggtctac acatccaatt ttatttaggg tggctgtatt
    45661 atttgtttcg acgtatcgga tggccactac acgtcagtat tattccgttg agtctactaa
    45721 ctatctgtta tgtttctatt gcaggaagtt ctatttctgt tttgcgtgct agtttgctgt
    45781 ttttattgcg attgctagtc aaattgctga ataagcatta ttccaccatg gacttatttg
    45841 cagttgtctt ttggcttctt ttactatttg atcctcgatt tctttttcat gcgggtggac
    45901 agcttagttt tttcatgagt tttttgttta tcctgatttc ttttgatgga gattggttga
    45961 aaaaaacggc ctctcacgtc ctgtttacta tgatcacgat gccgttgatt gtatggcatt
    46021 tttatgagtg gcctatttta ggtagtcttt ttacattact gattgcaccg gtattcaagt
    46081 ttctttttct tcctggtgtg ttatttctca cactcttcaa tcgctggctg ccggaaactt
    46141 tacttttgct gatagaagag ggattaatct tatttgagcg aataatcgat ttttcttcag
    46201 ggtcgtctct cattatagga cgtttgcccc ttgtgctatc tgggatgtat attttcggat
    46261 tactgatttg tatggatagg ttgaaagaac aacctgtgaa acacttgttt tatgttggtc
    46321 ttttttcttt tatttttttg actttgccat ttttacgctt ttcttcaacg atcgctttta
    46381 tcgatgtcgg tcaaggagac agtatctttt tgcaaagtcc ttttcgaaaa gaaacgattt
    46441 tgatcgatac tggaggtcag ttacactttg aaagagaaaa gtgggcgcaa ggtatgatac
    46501 gtcctcctgc tgaaagaaat atcgttccat atctaaaagg tcaggggata agtgtaatcg
    46561 ataaactcat cttgacacac gacgatacgg atcatgtggg agaattaccc acgttatctc
    46621 agcattttac tatcaaaaaa atttatatcg gttggggcgc tggacgaaat gagccattac
    46681 aaaggcattt atctgaagct caaaaaaatg gtactgagat cattgaaatc aaacatgggg
    46741 acaggatcag cggttattat gatctttatg tattgactcc ttttgaaaag ggagagggaa
    46801 gaaatgaaga ttcgattggt ctatggatgg aatataacaa taggcgcttt ttatttttag
    46861 gagatttgaa tcaagcaatg gaaaaacaat tgcttttgat ttatcctaat ctgaaagccg
    46921 atgtagtcaa attaggtcat cacggtagcc gtacgtcaag taacacagat tttctttccc
    46981 atattgaagc aaaacatggg attatatcct gcggcgtaaa taatcgttat ggtcacccgc
    47041 acagcgaagt attggagaca ctagaagaaa atcagataaa aacatggcga acggatcaac
    47101 aaggaatgat cacctatcgc tggcatccag tttttcatcc tcatgggcta gtggaaacaa
    47161 tgatagacta gtaattctca tctgctcatg gtaggataat ttttgaaagt ggtgagtaat
    47221 ttgagtatac aagcagagtt acaaaaaata cgtaaagaaa aacttgcatc ttgttacttg
    47281 attttaggaa cagaaaagtt tctccaagat caagttcgga cagaaattgt caaaaaaatc
    47341 caattagagg gcgaagatga cttaaatttt ttaagttttg acatggaaaa tgcaagttta
    47401 gatgaggtag tagctgaagc agagacgttg ccgtttttcg gagatcaacg gttagtattc
    47461 gtagagaatc cttatttttt aacaggtgaa aaagtaaaca atggaatcga acaaaatact
    47521 gatttattga cggcttattt gaaagaacca ttagaatcga ctgtattagt attctttgcg
    47581 ccatatgaaa agttggatga acggaaaaaa gtaacgaaac aattgaaaaa aacagcaatt
    47641 atcatcaatg tccaacagtt gaatgaaaaa gaagtacgtc aatatctgat gaatacctta
    47701 gagaattcct caatcaaaat ggaacgaagt gcaattgatt tattcttgag gttgaccgac
    47761 ttagatttat ccaaaatgat gagagagtta cagaaattgg tcctttatgg acaaaatcaa
    47821 cagcaaatca cggtaagaga agtagaagaa ttagttccta aaacgttaga acataatatt
    47881 tttgacatga cccaatatat tttaaaaggg aagacggaac aagctttgcg tttatacgaa
    47941 gatttagtta tgcaagggga agaaacaatc aaaattaatg cgattttgct ttctcaatta
    48001 cgtttatttt tacagacaaa atttcttgta aagattggtt atcagcaggc aaatattgct
    48061 gaaacattga aaatccatcc atatcgtgta aaacttgcca tgcaggaagt aaaaaaattc
    48121 gatgaacatt tgctggtaca gttgtttgat caattagttg agatggatta tcaaatcaaa
    48181 acgggacaaa tcgaaaaaga attaagtttt caactattcg ttttgcgcac agggcagttg
    48241 ttaagaaatc gataagaaga aagacgaggg caaagcatgc gggaaggtcg tttgtttttg
    48301 tccttttttt gacaaataaa aaaatcggcc gaagccgatt cttttattta gcgatttttt
    48361 tgcttaaacg agatttgtcg cggttagctt tgtttttgtg gattagacct tttgattcag
    48421 ccatatcaat agctttaaca gcttctttat ataacgcatc tacgttgtca gcacctgaag
    48481 ctacagcatc ttcaaatttt ttgattgcag tacgcatagc gcttgtttga gatgaatttt
    48541 tcacattagc gttttcgctt gtacgaacac gtttgattgc agattcaatg tttggcatta
    48601 gtttcacctc cgataactta agttcttacg cgtgttaccg cataattcaa cattcattat
    48661 tatacctaaa ggcagcgtgc tttgcaatat gaacatgaca agtttttttc cttaacagtc
    48721 ttttattagg attttttttc agtaaaaacg gatagtacaa gctgaaaaaa atcaaaaaga
    48781 cgctattgtg ggaggcaata gcgtcaaacc aaactagatt tttatttatc tatttaaaca
    48841 aagtttgaat gggaatgttt ggtactaagt tattatataa caaagagaac cgtttgcaaa
    48901 gttttttttg aaaagggata cgattccctg ttgttctttg tttttctatt aagaaactgc
    48961 agttatttta taaccagtat cctttgatca gtaaaggcta ttctggtgta ccgattattt
    49021 taatgtattc atcgatattt tttatcaaca ttttttttat gaccaactgg ttccgacggt
    49081 tccatttgcc acttacttcc agcacatatt tatttagggg caaacgtaag atatattttg
    49141 ccaaatagcg atctttaagc aggccattga tcacacggtc ctcaagctgc aagttgatcc
    49201 cagtcagcat cattggctgt tcttgtaaaa cgtacgtatc aattaaaaga ccttcaaaag
    49261 tttccacaag cgtcttcctt tcttttcaaa gtaacctgaa aacaggcact aattcttcca
    49321 ttttgatatt cgttgaagtg gatgcgatca ttctttcttt tgtccactgt aatctagtat
    49381 aacaaaaaag agcagaagag ccaaaaagaa aaaagagcat cgaattctat aggaagtctc
    49441 attggcacgg cctaaattat tacaaaacat tgattaaaaa aagcccctgc atcgcaggag
    49501 catcccctat atcgttttaa taggtcaatc aaaaagcagt atcaaacaca ttttgtatca
    49561 aatcaggaaa taatagataa gctaagccca gaatcaaaag taccgctaga agaaaccagc
    49621 cgattgttcc tatgaaatac tttaattttt cgaacattgt atcacctcgt tgtcagtata
    49681 tcacggatca gtaaattggg ggtgttaaga atctcctaaa atagacaaaa tttgttctat
    49741 tttcctaaag caaatacttg taagatacag taagtgatac cagaagcaat gaccggtccg
    49801 gcggcgattc ctcttaagaa gacaacacct aaaatcgtgc cgaaaacaag tgcaacagtg
    49861 atttgtggat cagaagcaat caatcctaca cccttggaag ataagacagc cactagccca
    49921 ccgcaaaaaa cagctaccca gccaataggg gatttaaagg cttctaatag atcacgtaga
    49981 ccgatttctc ctgttgcgat agggattaga atagccgctg tgatgatcgt caccccaata
    50041 ttgattcctt tgccttgaag ccaagggaaa aaggaacggg aggctggtat gagcttcata
    50101 atcaaaacaa aagctgccgc tatcatcaga ctttgatttt tagctaaaat tgctacaagt
    50161 aaaataattc ctaaaaaaag ccaactttcc atgaaatata ttcctcactt tcttcgaaac
    50221 aatcataaca tgtttttaga aagttacaaa agtaggaatt catccttcaa gacctattca
    50281 taagctgttt ttttcataaa ataaacaaaa tcataaaaaa acactttgct tatttgacag
    50341 aagacagccg cgctttgtat agtggacaag acttacattt ttttgaagaa tcagaaagat
    50401 caggtaaagg tcttagtaaa cgcaagccat tcagaataac caatattgtg cttccttcgt
    50461 gtcctacaac tccaagaggt agatttaaaa attgcaggaa gttagaagca atcaacaaga
    50521 gaataacaag aatcgaaaaa ataatatttt gtttgatgat ccgccgcatt tttaaagata
    50581 gccgatgact gtagacaaat ttatttaagt cgcttttcat taagacgatg tctgcgatat
    50641 ccatagcggc atctgtccct tgccccatag cgacacctaa agaagcggct gctaaagcag
    50701 gcgcatcatt gactccatca ccaaccatcg catttacttg gtaaagttca ttctcttttt
    50761 tgactagttg tgttttttct tccggagtac agcttgcata gtaatgttca atccctactt
    50821 gttcagcaat ggcctttgct gtgccagaat ggtcgccggt tatcatactt gtatggatgt
    50881 tttgggattt aaagtaggaa attacttgtt gggtatttgc ttttggaata tctagcagac
    50941 ctaaaacagc aattaactgc tgatctttag aaagataaat cacggttttg ccttgatttt
    51001 ccaaacgttc gatcatttcc gcaatctctt tgctgattat catcgtctca ggatcatatg
    51061 cgtgcttgcc aactttccat tgcgcaccta aataggtagt ttgtaatcca aaaccagtaa
    51121 tttcttctat tggtaagtat tggatttctt caggtattgt gcaatcgaaa tattgcaaaa
    51181 tcgcatgagc aagtggatgc gtcgtttttt gttccatagc aacaagaagt tgttgtgcca
    51241 aatccttttc atcaaagaaa aagctgtcag ttacaactgg ttttccttta gtgagcgttc
    51301 ctgttttatc aaaggcaatc gcttttaatt cagaaagctt ttctaaatgg ataccgcctt
    51361 tgattaaaat cccatttttt gcagaatttg aaagagcagc tagagaagct ggtgtagcag
    51421 atgcgacaag cgcacaagga gaagccacaa ctaataatac cattcctcga taaaagcttt
    51481 catcccaact ccaacctagg aaaaagtggg ggagaaggat cattagagga acagcaagca
    51541 aaatcaattt tacataatta ttttcgattc gttcgataaa tgaagctgtt tgagttggcg
    51601 tattctgcgc ttcttctact aattggataa tcttagcaaa gacagtttcg gaactcgttt
    51661 ttgttactcg aatcgtgatg ggatttcctt gattcagcgt tcctgcatac atctcatcgc
    51721 ctaaatattt ttctacaggc acagattcac cgcttaatgc agactcatcg atcgttgcct
    51781 gtgtgctttc taaaaagcca tctatcggaa cagctgctcc tttagcgaca aataccaagt
    51841 tatccacctt tagttgagaa acatctactt cttgcatttg tccattttct tttaataatt
    51901 gggctttttg tggttgaaga ttcatcaaag cagtaatttc tttttgactt ttattagttg
    51961 tgtattcctc taatgcaccg cttaaacaaa agatgaatgt caacattgct ccttcaaagt
    52021 aattaccgat caaacaggca ccaatagcag ctaaagccat caataaatcc acattcagtg
    52081 tccgatcatt ccacaattct ttcaagcctt ccgatgtttg tttatatcca ccgatgataa
    52141 tggcactgat ataaaaaagt ggggtgatgg taagagagaa tcggtcgaag ataaaaccga
    52201 agatcataaa taaaaggcag ccgattgttg ctgcaaaagc ttttttctga ttagtcatcg
    52261 ttattagacc tccttgtcaa aacaaggata gcataataaa aatgtaattg ataataataa
    52321 cagctaaatg agaacgataa tggttatcat ttggtgatta agcttgatgt tgtttttatt
    52381 atttataatg attattatta aagaaaataa aaaaccagaa catttctcaa ataagagaag
    52441 tgttctggtt ttttctacat tgcaataaac caataaccaa tcaaaatgat accaaggata
    52501 atacgatacc agccaaaagc ggtaaagtcg tttttcttca aatagcttag taagaatttg
    52561 atagctaaga cggaaaccac aaaagatacg aatgttccta caagaagaac aacagtttct
    52621 tccattttga atgaattccc ttcagctaaa tatttgacga tcttcaagcc gcttgcgcca
    52681 aacatgacag gaatccctaa gaaaaatgaa aactctgtag cgacaaaacg cgaagcacca
    52741 atcaagatag atcctaggat cgttgcgcca gaacgggatg ttccgggaac aagtgccaag
    52801 acttggaata acccaacaag aagagctgct tggtacgtaa agtcattcag agaagtccaa
    52861 cgaggtgttt tgtttttgtt gcgtttttcg acaataataa aggcaatacc gtaaacgatc
    52921 aaaacaatgg caactggtaa gaacttgtgg aaatttgctt ctaaccaatc atccaaaggc
    52981 aaaccaatga tgatggctgg tatgcaagca acgatcactt taaaccaaag tgtccacgta
    53041 tctttttttt ctaccgttga tttacttgga gaaaatgggt ttaacttgtg aaaataaaga
    53101 acgacgacag ccaagatcgc ccctaattgg ataacgacat tgaacatcga cataaattgt
    53161 gaactggcat ccagtttgat gaattcatct gctaggatca aatgaccagt actactgatg
    53221 ggcagccatt ccgttattcc ttcaataata ccgagaatca ctgctttgat aatatttgca
    53281 aaaaacataa attccttcct tcccaattaa gatacatctt atagtatacc gttatttttt
    53341 gagaaaccca atctgttttt taatattaag aaaaacttga gaaataaagc agataagtac
    53401 acgaagagaa aaaagaagac aaagccctcg ttcagacttt gccttttatg attctctggt
    53461 aaaaattaaa atatccccat tatagaaacg agtcgcgact atgtgttttc ccgttatcga
    53521 tataagacgt atcattgatg ccgtggatag aaaatcgacc gtttgcataa gtgacctttg
    53581 ttacgctgcc atttaaaagc ccagcacgaa caggctgttt aggatcgatc agattaagca
    53641 aaaaggcgat cgtcaaacca tgagatacga ctaaaacatt tccaccacca tttttttcgc
    53701 agtgatgggc gatatcttca aatcctgtcc atacacggtc tcgcaatgtt tcataagttt
    53761 ccgcccaccc agcggtatct aagttgtata cagcattagc aatttgttca aaagaaactt
    53821 ctgtggtaaa catttcatca tatgttttaa aattcaatac acgagggatc acaccccaca
    53881 tttccatgtc atatccacct tcaagagacc cgaaacacca ttctctaatc cttttttcaa
    53941 taaaataagg aatttctttt cccttagaat gttctcctag aataatacgg gcagtttcga
    54001 ttgcacgacc actgtcacta gaataagcaa atgaaaagtc gacatttttt aacccgcgac
    54061 ctaaataatg aatgccttct tgtccttgct ttgtgagggg tgtatcacac cagccttgta
    54121 ctcgttcaat tgtattgaac atcgtttttc cgtgtcgtac gatataaagt gtagtttctg
    54181 tcattttatt ctcctcctaa gtcgttttaa cgattgaaaa aaggatttgt ccttttttcc
    54241 tgttcaatcg tcgttgcatc gccatgtcct ggatagactg caaattcatc aggtaataca
    54301 aacagctggg tccgaatact atgaagtaat tgttctaaat tccctgtata cagatcggtt
    54361 ctaccgatac ttcctctaaa cagggcgtcg ccagaaacga caaaatcatc aaaaataaag
    54421 ctgacgcttc ctatagaatg ccctggggta gggacgacgc gaaaagacat gtttcctaaa
    54481 gtataagttt tcatttcaaa ttcatattct gcaggcgaga caataacatc agcaatatca
    54541 tcatgtctac cgagtccaga aagattcaaa atcggatcac ctagccattt ttgctctagt
    54601 gggctgacat aaactgggat ttgataatac gcgcgtaaag gttccactgc accgatatga
    54661 tcgtagtgag tatgggtaag caaaatcgct accggctgtt ggtttgtttt tttgatttct
    54721 tgtttgattc gttccgcatc ttctccaggg tcaatgatca aaagtgcttc ttcgttgtag
    54781 accaaataac agttttcttc aatcattcct gtttttaatc tttcgatatg catgtgcatt
    54841 cctccttgat agccaaaact tctttcatcc attagtataa cgcattcatt gctaggaaac
    54901 aaaaacaggt acttatttct ctttacttat ctgaattatc agactatatg aaaagtattt
    54961 ggatattcta aagagaagga aagtaagttt tcatagcatt ttaatatgtc cttgttacat
    55021 tagattcatc caaaaatcca aattctatat cctatatccc tgcctaatca gcagggtgtt
    55081 tttttatgtt tggaaagaga gctctttttg ctaaactagg ttttttcagg taatcttaga
    55141 gagaaagagg tggtggaaat gatcaaacag tcaaaaatat ctgtgcggca cttagtagaa
    55201 tttattttgc gacgtggaag tatcgacaat cgtaaaaaaa gtaaccatac tgcattagaa
    55261 ggagcgagaa tccaccgcaa gctccaaaaa gatgcgggag aaaactatga aaaagaagtc
    55321 tttttaaaaa caacagttca attagatgaa tattacctga tcgttgaagg ccgtgcagac
    55381 ggtattttca aaagagacgg tatttattat atagatgaaa tcaaaacctc cgaaccacga
    55441 tttgaagatt tagaaccaga acaagtggaa cttttctttc atcaagcaag ggtgtatgcc
    55501 tatatctata gtcaagaaca tgatttacaa gaaataaatc tgcaattgac ttacttccag
    55561 acggcagaag aagtgatcac gagaaaggtt gaacatcaga caagagagca gttagaaact
    55621 ttttttaaaa agctgactga agattataaa gaatggcttg tttttcaaga aaactggcgc
    55681 actgtacgca atgcctcttt gatggctttg aaattccccc atgatgagta taggaaaggt
    55741 cagcgtgaac tagcggtagc tgcttataaa acgatccgta cgaaacagaa attgtttgta
    55801 gaagcaccta ccggaacagg aaaaacaatc tctacgttat tccccgcttt gaaagctgta
    55861 ggggaagaag agggagaaaa gatcttttat ctaacggcta aaacaataac tagacaagta
    55921 gctgaagatg ctatgacagc attaaaagat acaggagctg aagtaaaaag tgtcactttg
    55981 acggcgaaag acaagatctg ctttttagat gagacgacct gtaatccaga tcagtgccca
    56041 tatgccaatg gttattacaa tcggattaat gaaggtttat gggatctttt gaatcatgag
    56101 aatcagatca ctcgtgaagt catcgaaact tatgcaagaa agcatacact ttgtcctttc
    56161 gaactatcat tagatgtcag tatctggtgt gacgtaatca ttggggatta caattattta
    56221 tttgatccaa ctgtttattt aagacgtttt tttgaagatg aaaaaaacga ggactatctg
    56281 tttttgattg acgaagcaca taatttggtg aatcgttccc gagagatgta ttcggcagaa
    56341 ctatcttatg aaaaaacgaa acgaagtaag gaagctatac caaaggaatt caaaaaactt
    56401 catcgccgtt tcaacaaact attaaaagaa tttgattcga tccgagaaat agcaaaagaa
    56461 gatcattggg attatcatca tcaaaaagca ccggctgaat ctttagtaaa ggcaggttat
    56521 caattaagcg aaaaaattaa agaatggctg gcagaatttc ctgagcaccc atcacaagaa
    56581 caattgcttc cttattattt tgatttgctt cattttttga aggtcagtga attttatgat
    56641 gatcactacg aaacaacagt cgaaaaaacg tatcgcgatc tgatagtaaa agagttttgt
    56701 attgatccaa gcttgtttct tgagcagtcg ttggataaag gaaaaagcag tattcttttt
    56761 tctgctagtt tttcaccact ctcttattat caggaaacac tggggggaca aaaaagtctg
    56821 gcttacaaac tgccgagtcc tttcccagaa ggaaaccagc aagttttcat cgcaaattac
    56881 ctagagacga cttatcgaaa aagggaacac agtctggcaa aattagtaga gacgattcat
    56941 gcttttgctg atggaaaggt cgggaattat ttcgttttct ttccttctta tcaatattta
    57001 gatcttgcgg tggaagcatt cagacagcaa tatcccgaca gcaatgtgct aatccaagaa
    57061 acggacatga atgaggaaga acgcgaacga tttttaacaa aatttcaaac aaatcccgct
    57121 gaaacattga ttggattttg cgtacttggc gggatttttt cagaaggaat cgatttgaga
    57181 ggggaccgat tgatcggcag tatgatcgta ggtgtaggtt tgccgcagat gaatcatgag
    57241 caggaactga tcaaatcata ttatgatgag aaggaacacc ttggatttgc ttacgcttat
    57301 cagcttcctg gaatgaacaa agtcttgcaa gctggtggtc gagtgattcg tggcatggcg
    57361 gatcaaggaa tcgttgtatt ggcagatcaa cgattttcta gtttttctta tcgacgttta
    57421 ttcccacaac attggcaaac agcaagagaa gtacgaagtg ttggggaatt ggaagaagga
    57481 atacagcgtt tttggttatc aaaagaagca gtaaatcaaa aaaacgagga atgacagacg
    57541 tcagcctcgt tttttgatta acgatagtta gtgaattgca actcgattgg taagtcaagt
    57601 ttacgtaata aagcaatcac agcttgtaga tcatcacggt tcttacctgt cactctaatc
    57661 tgatcttctt ggatttgtgc ttttactttc agtttttcat ttttgatagc tgtagtgatt
    57721 ttcttcgcgt tttcacgatc gattccgttg accaaatcgg cagtttgtcg agctttgcca
    57781 cctagagcgt gttctgattc ggaaaaatgg atatttttga ttggcacatt tcttttgatc
    57841 agcttaccaa ataatacatc tttgatctgt tcgattttga aatcatcatc gctcaataca
    57901 actagattgc tgttctctaa tttgatttct actgtagaat ccttgaagtc aaagcgattg
    57961 gacaattctt ttgttgtttg ctgaatcgca ttttttactt cttctgtatt gacttctgat
    58021 acaatgtcga agctagcatc ttttgccata ggaaccctcc tgatatacat ataaatattt
    58081 accatacatc tatcttatca taggattagg gcagattctt acctgtttgc taaaattctt
    58141 ctcatttgag ttcgaagtat tttattttaa tagataaact gttattctta catttggaat
    58201 accaaagtag gggagaggtg cttgtgagta aaattatgac tgcgtatcat aaaaatgaaa
    58261 gattaaagaa aatagtggtt attttgataa ccggattgac cgctgcggtc gctttaaatt
    58321 attttttgat tccagccaat gtcttttctg cagggatgaa tgggatcgct caaatcattt
    58381 cctcgctttt atatgaatgg tttcatatca agatagacac aggaacattt atttttcttt
    58441 tgaatatccc tgtgtttgta ttagggtttc tcaaagtagg aaaacgagca acgatcctta
    58501 gttttgtgaa tgttgtatgt gtatcggtga tgacgatgct gcttccagtc ggacagatca
    58561 ctgataatat tttgatgaat gccctagtag gcggtgtttt attaggtctt ggcgtaggat
    58621 tatctttaaa aatgggcttt acaactggtg ggatggatat catctctctt gtactttcac
    58681 aaacaactgg aaaaacagtc ggaaactaca tgctgattct aaatggtatc attgtagtca
    58741 tcgcaggctt cttctttaac tgggaaagtg ccttgtatac gattatttcc atttatgcaa
    58801 tgactcaagt aattgatgcc atccacacga gtcatcaaaa agtaacagca atgatcgtta
    58861 ccgtcaagcc agaagaggta acagaagaac tagcaaaacg gatggtacgt ggaatgacat
    58921 tactgccgtc tgtcggtggc tattcaaaaa aacaagggaa catgatcatg atggtcatca
    58981 cccgctacga aatgtatgat cttgatcaga tcgttcatga aatcgacgac gatgcattta
    59041 tcaatatttt accaactcat tcagtatttg gccggtttgc aaatgaaaac gaacaaaaaa
    59101 tgtatcgaac aactggcgta ttgccagaaa taaaaaatcg ttcacaaaaa aaatgaggaa
    59161 tcaggtttat tttagaataa atttaatcta ttacatctaa taagaacatt tttataaatt
    59221 taaacgagaa tcttacttct aaattctcat ttacaagcag ctaaaaaaag agtatgataa
    59281 ataaaaatcg tgttaaggag tgtcgtacga tgagcgaagc aaaccaagaa cggtctggac
    59341 aagaaataga actaatcaaa gaacgtattc ttgctgcctt ggaaatggtg attgatcctg
    59401 aattaggaat cgacatcgtc aatttaggct tgatttatga tatagaattc aatcctgaaa
    59461 atggagaaac agtcattaag atgactctaa caactatggg ctgtccatta gcagatattt
    59521 taacagaatc tatccatgac gcattaaaag aagtgccaga agtaacgaaa gcagaagtca
    59581 aactagtctg gtatccagca tggacgacag ataagatgag ccgttatgca cggattgcgt
    59641 tggggatacg atagaaatct tggctgacta gctatgagaa gcaaatagga aaaacaaaaa
    59701 agccttttat cctttctaga agaaaagaga taaaaggttt ctttgtttat aaaattttgt
    59761 ctgctatatt ataagttaat taaataaatg tgttctattg ataaaggaca ttcaaatccc
    59821 tttccttttg gaatccttct tttttacatc agtttgattt tcgtatagta ttctttctca
    59881 tcttcacgat aaatcgcagc cccatttttt tgttgtgctt taagactgcc aagatttttt
    59941 ttatttacag acaaaagtgc ctcatcaatg ccgatttttt ttgcctcttg tagtactaat
    60001 gctaatcctt gtgtggcata tcctttttta cgataattag gggagatgcc gtaaccaata
    60061 tggccagcgc cgtttcttaa tgtttcgttc agtttatggc gcaaattaaa aattcctaca
    60121 tactcatgtt catcactttc taaaataaag acggttgcgg gaacgtaact ctcaggaaga
    60181 tcgattccta aagacgtttt ttcgcatttt tttacataat ctaaaaattc agcaaatgta
    60241 tagccataag cattgttgat aaatccactc tcatttggtc caaagctagt ttgtagctga
    60301 taagctttct ttaaatctgc ttcccatagt ttgatcagtt tcatcgtatt cacctcttta
    60361 agtaacctta ttcatttgtt ttctactata gcactattgt tgtaaatagc catgcctatt
    60421 tttatttatt gtgtcatatc gtattaagaa tcatcgaaca atacaaatat gaattacaat
    60481 aaaaaaatta tttgaagttc gttcattcgt tacttatgat ctctagaaga aagaaaacgt
    60541 gaattacttc agcgtctaga attccagaag gatagtaatc aacagaagaa ataatcaata
    60601 tccaaatgaa agaagatagc tgaatcgaga aaaagaggaa aaaagaaatg aagaaaaaaa
    60661 taaaatagtt actaatccca gagtcgttcg tttaacattg aaatcgatca tatggataaa
    60721 acagattttt tcaccaaccc tcaatagatg atggagttgc tcggatgaat tatttgtgta
    60781 gatggcataa gggagggcgt acagaaatga aaaagaacaa aaatatgttc ttgacaaaaa
    60841 acacatgtaa tcctaaacta aaagatgtga tgcggctatg gcggaattgg cagacgcgct
    60901 agcttcaggc gctagtgagg gtgacctcgt ggaagttcga gtcttcttag ccgcataatt
    60961 tgttttttta taaagagaag gtacacctgg agcgatgaga aagaaaataa catattatct
    61021 aaaagaatat aggaaaaatt ttaaacaacg gaaccagatt caaataaatc aaaaaaaatt
    61081 tgtgtgaagg tgggacagtt taaaacctaa tagattgatt caaatagtag atttatagat
    61141 tctgatacaa gaaatactgg caaacgatag ttaaggaata aagactaaac ttaaactcaa
    61201 aaagaggtca tgcgaaagaa taaatcgcat gacctcttac tatattttga atgtgtttaa
    61261 acgttcaaat aatgaatctt agtaccaacc gtttgcttcc cagaatgctt tcgccgcttc
    61321 ccaagaacca taacgtgaag taacatattg ttctgctact ctttcttggt ttgcagcaga
    61381 gtagtcacca tttaagtaag aagaatctaa ttggtaacgg ccgatgtaac gtccgttcgt
    61441 tgctgtgtaa gaaccgcttg attctttttg tgcgatccat tcttttgcag agcttgtact
    61501 tgctggagta gcagcagttt ctgttacagc aggagcaggt tgtgtttcag caacaggcgc
    61561 tgcttgtgtt tccgtttgaa ccgtttcttg tacaacaggt tgttcagctt gaacaggttc
    61621 agcttggact ggtgcttctt gaacggctgc ttgaggttca gcagctgctt gtgcagagcc
    61681 ttctgttggg attgttaatt gttgtcctga gta
//
LOCUS       DAZKLM010000005        53655 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3764
            SAMEA115706913-rid22582563.denovo.005, whole genome shotgun
            sequence.
ACCESSION   DAZKLM010000005 DAZKLM010000000
VERSION     DAZKLM010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706913
            Sequence Read Archive: ERR13196647
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 53655)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 53655)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287823.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287823.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,004
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,849
            CDSs (with protein)               :: 2,849
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 41 of 96
            Pseudo Genes (incomplete)         :: 52 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 15 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..53655
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706913-rid22582563.denovo.005"
                     /isolate="VRE3764"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..878
                     /locus_tag="ACG842_000262"
     CDS             <1..878
                     /locus_tag="ACG842_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HFQ0246832.1"
                     /translation="SLSQLLPEVVCFDEFKSVKNVSGAMSFVMMDGKTHQLIDIVENR
                     QLNPLRDYFLRYPIKVREQVRLVVSDFYTPYRTLVKECFPNARIVADRFHISQHIGRA
                     FTNHRIQVMKTFKKGDRRSKHLKKYWKLLQKNAWELKGQHRYWRPSFRDHLTEAEIVD
                     RLLSYDDSLKRGYEVYQDFLSVIRRQDVPEFVALLKEDYKELPEHYQPVFTTFKKYQT
                     EIKRALRVPYSNGPIECLNNHIKVLKRIAYGYRNFQNYRERIFLYRGKYFKKTKNTTQ
                     LTKARTTTRLDKIAV"
     gene            complement(1119..2735)
                     /locus_tag="ACG842_000263"
     CDS             complement(1119..2735)
                     /locus_tag="ACG842_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002406986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFQ0246833.1"
                     /translation="MKKIPITILTGYLGSGKTILLNHILYGDHGKEIAVIVNDIGEVN
                     VDGVLVDKKGFKRTGEKLVSMPNGCICCTLREDLIVELDQLVQFSDLNQIVIEASGIS
                     KPIPIIQAIILAKNPLDIDLTQRLEIGAIIIVVDVKRFWDNFETGEKIQERKAEGNVV
                     EEEQDIRGLLIDQFECCNILLLNNSDLVSTKTIYKINGLIAKLQSNAKIIRTIKGEVD
                     LSKILNTNLFNIDETSYSVGWFKELELGYQNHIPETEEYGISSFIYRNRLLLDAQKFA
                     HLTDKNFPGNITRAKGFLWFAQHSDFSTIFETSGSYKSIESLNYWVASLPKIECQMII
                     QKNPELKEAWHPLFGERINKLVFIGMKMDQQCIKEQLEGCLTDSLNADNFRKISPFPW
                     KKSNMFSQISLMKFLRKAGVKIGDVIIIGILIITSFIPLFVFSIEKENETTEDAIKYA
                     IVRIDGKEMDRFDLDKINHKLVNYHPAKGQYNIVEIKDGRIRVKEDNSPDQIAVRTGW
                     ISKPGQTSICLPHKLVISIEKKESTDYYIY"
     gene            2853..3071
                     /locus_tag="ACG842_000264"
     CDS             2853..3071
                     /locus_tag="ACG842_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0246834.1"
                     /translation="MSQYRHLTILERERIFLLHEEGFSISIIGNDIQHSPFTILHELR
                     RNKKKTIHLPKRKKIMLIEEKNADVITF"
     gene            3266..3727
                     /locus_tag="ACG842_000265"
     CDS             3266..3727
                     /locus_tag="ACG842_000265"
                     /inference="COORDINATES: protein motif:HMM:NF033563.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFQ0246835.1"
                     /translation="MHHRGKTRHTKNHVESRGKICLTHSIHGRPKSADDRSDFGHWKA
                     DIVAGKTGGTCLVTLTDRKSCFLLAGKKVPRKKAAYVSEKMIELLSSIPNEAVKTITP
                     DRGKEFSNHFAVTEALNNMPFYFPDPHALGNNEQIKIRIDSFENTCQNRKR"
     gene            complement(3920..4159)
                     /locus_tag="ACG842_000266"
     CDS             complement(3920..4159)
                     /locus_tag="ACG842_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0246836.1"
                     /translation="MVRKVLLSMDSKNIDAYRRYRFAKQMRLNGSTLADIAATLHVSI
                     DTVHRVLKRDPDCCFIKVNTGRSRRLAKRHNSLAN"
     gene            complement(4183..5706)
                     /locus_tag="ACG842_000267"
     CDS             complement(4183..5706)
                     /locus_tag="ACG842_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HFQ0246837.1"
                     /translation="MKKISVGLIGIAALGLLGACSSTNDAKVSNDKDGKLEIVTTFYP
                     MYDFTKNIVGDEANVDLMVPAGSEPHDYEPSAKDMAKAHDADVFVYHNENMESWVPKA
                     KESWKKAGPNVVEGTKDMILLPGSEEEDHDHGEEDHHHELDPHTWVSPKMAIKEVSNI
                     KDQLVKLYPKKAKVFETNAEKYLTKLKRLDADYTTSLKEAKQKSFVTQHAAFGYLALD
                     YGLKQVPIAGLSPEEEPSSGRLAELKEYVKKNKINYIYFEKNANDKIAKTLANEAGIK
                     LEVLNPLESLTKEQMDNGEDYVSVMEDNLKALEKTTMVAGEEVVPEKEAKDEKTVANG
                     YFKDADVKDRELSDYTGEWQSVYPLLKDGILDEVFDYKAKINKDMTAAEYKDYYTTGY
                     KTDIDTINIKDNTIDFVVNGEHHQYTYKYVGYKILNYEKGNRGVRFNFETDDAGAGRF
                     KYIQFSDHGIAPSKAEHFHIFFGGESQEKLYNELHNWPTFYPASLSKHEIAQEMMAH"
     gene            complement(5724..5846)
                     /locus_tag="ACG842_000268"
     CDS             complement(5724..5846)
                     /locus_tag="ACG842_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HFQ0246838.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKEHKRNKMKKLA"
     gene            complement(5876..6859)
                     /locus_tag="ACG842_000269"
     CDS             complement(5876..6859)
                     /locus_tag="ACG842_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFQ0246839.1"
                     /translation="MGIPVTIVSGFLGAGKTTLINQALQVSPYPREEIIIIENEFGEV
                     GIDHKLLVHSEERVLQLNNGCMCCSLRSDLLAILISLLETIEEHQQPISQIIIETTGI
                     ADPQPIIQTLLTAPQLRGRVYIDSLLTVIDSDSFELIEREPQALKQLVMADRIFVSRK
                     QNLNLSALKKLQREIKSINPLSDIRMFTHSETIQKKDFYNLKKFNHPLVFESDSSENH
                     YNGKYYHQHHQVHEFKSILLTSDQDIKKDFLLQWIDWLILTNQGSLYRFKGLIALQEQ
                     DFIVAMQEVNEQVNFQYTTRQKDQTTIILIGKMLDEERIKQSFDKLLAESK"
     gene            complement(6883..7032)
                     /gene="rpmG"
                     /locus_tag="ACG842_000270"
     CDS             complement(6883..7032)
                     /gene="rpmG"
                     /locus_tag="ACG842_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HFQ0246840.1"
                     /translation="MRQNIILECVETGERLYLTSKNKRNNPERLELKKYSPKLRRRAI
                     FKEVK"
     gene            complement(7053..7430)
                     /locus_tag="ACG842_000271"
     CDS             complement(7053..7430)
                     /locus_tag="ACG842_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0246841.1"
                     /translation="MEIIYPPLVEEGLKYYLETTQQSLDKCTFYRSMVERGIITETGL
                     PTQQAIENGLVKDYYEDQGLSFDDFLRIYPIFEEYDEELFQCIDGYWEIPVDMKENLV
                     SQLESGELTFEDAQQIQAYLEDR"
     gene            complement(7462..7641)
                     /gene="rpmF"
                     /locus_tag="ACG842_000272"
     CDS             complement(7462..7641)
                     /gene="rpmF"
                     /locus_tag="ACG842_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018345355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HFQ0246842.1"
                     /translation="MAVPARKTSKAKKRLRRTHQTVVKPEISFDEANGDYRRSHHVSL
                     KGYYNGKKVIKGASK"
     gene            complement(7656..7925)
                     /gene="rpsN"
                     /locus_tag="ACG842_000273"
     CDS             complement(7656..7925)
                     /gene="rpsN"
                     /locus_tag="ACG842_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HFQ0246843.1"
                     /translation="MAKKSKIAKAKKQMAMIEKYADKRQELKAAGDRTALAKLPRDSN
                     PNRLRLRDQTDGRPRGYMRKFGMSRIKFRELAHQGLIPGVKKASW"
     gene            complement(8448..10214)
                     /locus_tag="ACG842_000274"
     CDS             complement(8448..10214)
                     /locus_tag="ACG842_000274"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0246844.1"
                     /translation="MQMIGGLTMKSFYKNKFALSDQGAEALTKASISSFFVYCINMVP
                     AFIIMMLIDELVLENTKPRWLYFAVSFVTLLFMYWLLDREYENLYNSTYKESAHLRVQ
                     IADDLSNLPLSYFSKHNLSDLSQTIMSDVEGIEHAMSHAIPKAGGMALFFPFISVMLL
                     VGNVKMGLAVILPTLFSFVLILLSKKSQTKANTKYYDTLRENSEEFQETIELQQEINS
                     FNLSKKVQDRLFKKMEESERIHLKVELSTFSVMALSSIFSYVSLAVVILVGVHLLLTG
                     EVTILYVVGYLLAAIKIKDSFDSMKEAVLEIFYLAPKIQRIRAMKETSIQEGSDSPLK
                     SFDIELRDVSFSYDNNTPILDHISFTAKQGEVTALVGASGSGKTSILKLVSRLYDYDE
                     GCILIDGYDIKRVSPASLFSKIAIVFQEVTLFNTSILENIRIGNSQASDEEVKKAARL
                     ANCEDFIEKLPDGYHTLVGENGSSLSGGERQRLSIARAFLKNAPILILDEITASLDAE
                     NEKRIQESLNRLIQDKTVLIISHRLKSIEKVNKIVVMDQGKLVDQGTHNELYRRSEIY
                     KNLIKKTKLSEKFVYEKEAQSR"
     gene            complement(10174..11928)
                     /locus_tag="ACG842_000275"
     CDS             complement(10174..11928)
                     /locus_tag="ACG842_000275"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0246845.1"
                     /translation="MKVYKKLFSYVPKMKYYGWLATLFSDFSVVLTVYGYYSIYKFLQ
                     SLIIVGDEYLAKSYAVQTVAWLTLGALFYIFSGLFSHILGFRLETNLRKKGISGLTEA
                     SFRFFDLNSSGYIRKTIDDNAAKTHMAIAHLIPDSAQAIVTPVLSVALGFFINFKIGL
                     VLIAMLVIGVFLLKKMTENTNFIQKYQESLDVLSSETVEYIRGIQVIKIFGAKVTSFK
                     ALNKAINDYAKFAYKYSLSGKTPFVLFQWLFFGIVCILIVPITFFITSLTTPKILAVD
                     LLMLFFLSGIIFVSFMRIMYVSMHLFEARYAVETLDSLYLEMKQDQLKYGNETQIKNC
                     DIAFENVSFSYKEECVLENFNLVLEQKRIYALVGKSGSGKSTIAKLISGFYNVDKGQI
                     KIGGKPLNHYSKDTIINTIAFVFQDTKLFNQSIYDNVSIANPQAPASKVFDALRLAGC
                     ESILEKFPEREHTLIGSKGVYLSGGEKQRIAIARAILKDAKIVIMDEASASIDPDNEY
                     ELQKAFQHLMKEKTVIMIAHRLSSIKSVDEILVLSEGKIVERGNHNELMAKHQVYKQL
                     VTNYENANDWRLDNEKFL"
     gene            complement(12038..12607)
                     /locus_tag="ACG842_000276"
     CDS             complement(12038..12607)
                     /locus_tag="ACG842_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HFQ0246846.1"
                     /translation="MKTKDYIFIGLATVISLVIYFFSIMVSSIGGAFGHSISPGIFGL
                     LSGIIFVYISYNYPRKGIFTIYTIVLLLFFTLMGGAYLPWFISSISMAILADIILSVF
                     GYDRAIPQVASWALMQLGSAAGQWIPIWFFTDRFRQDWIDKGQSAATMDAMIHYAVGI
                     WGIISVLVVASLSMIGVLIGRKVLKKYKK"
     gene            complement(12625..14040)
                     /locus_tag="ACG842_000277"
     CDS             complement(12625..14040)
                     /locus_tag="ACG842_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0246847.1"
                     /translation="MFEFNNVSFSYKDVKEQALVDLNFSIKKGSLTLITGSSGSGKST
                     LLKLMNGLIPRLYDGKLVGEILFENNSLQSLTQESISKNIGYVSQDPRGQFFTTNTTS
                     ELVFSMENFGFTKEHMDKRINELAELLDLSNILDKNIFSLSSGERQKISIGCSLSLNS
                     KLIILDEPSSNLDFKATKKLADLLCDLKNKGFTIVIAEHRFYYLKELIDQVLFVKNRT
                     VKTTTIQLLKTENNNALRKFDIFNSSIEVPYIEKVSADVLKVEEITYENILKDINFDV
                     KVGDVIGLVGKNGVGKTTLLRILMKIIRPTKGKIIENKKNLSSPFLVMQEMDYQFFTE
                     SVRSELALGNEIVSKDQQEKILKKMELYKMKDQLPFDLSGGEKQRLLVSIASLSKTNL
                     FLFDEPTSGLDYINMERIADLIKDLKEKGAVVIATHDPEFLYKTCNRIIYLKNGGIKK
                     DIKLRVEDSTLIEQIFNDITK"
     gene            complement(14051..14719)
                     /locus_tag="ACG842_000278"
     CDS             complement(14051..14719)
                     /locus_tag="ACG842_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HFQ0246848.1"
                     /translation="MSKINPLTLITLNIFFPVIIFLGKGNIFQVGCFIIATVLVLMFR
                     KIKTCIYFILIYSVLFLVMKIIDAIKIEFFTIFFATSLYIVFRMLPVVMISWLLVSVY
                     TSNELLSSLEKIHLPKKIMLSITVVIRFFPTYRSEIRMIKESLKMRNIRLSFFQPLQY
                     LEFLLVPVLIRATMIAEEMTANAITKGIESPVKRSSYYKTNMSLLDYIILFSVTIFFL
                     SILI"
     gene            complement(14849..15433)
                     /locus_tag="ACG842_000279"
     CDS             complement(14849..15433)
                     /locus_tag="ACG842_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFQ0246849.1"
                     /translation="MESGLSHFEKEGFQQTKIRQLCLTAGVTTGAFYKHFTSKEALFE
                     EIIQPHINALINMYNEEFTKYLLNLENIDIEKSWRENFNELDPFINYIFENKRIFDLI
                     LFQSAGTKYQDIVEKITSFVTKKTVCCLDKLKTIGMINQNLVFNEEEIHFYIYSFYAT
                     FYDILKHSYSKEKTMLLTRRLYQFSVPGWIELLS"
     gene            complement(15764..15997)
                     /locus_tag="ACG842_000280"
     CDS             complement(15764..15997)
                     /locus_tag="ACG842_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HFQ0246850.1"
                     /translation="MVQIKTTTILIYLLAIQELSKRKKKLKNNDLAKVLSVSPASVSE
                     MLAKLRYEDYLDIGFQLTDKGKKFIDDYKKGFK"
     gene            complement(16012..16962)
                     /locus_tag="ACG842_000281"
     CDS             complement(16012..16962)
                     /locus_tag="ACG842_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding
                     lipoprotein"
                     /protein_id="HFQ0246851.1"
                     /translation="MMRKWKAVLGSLGILIALFIFGACSINSKDKDKVASNEKLKVVV
                     TNSILADITENIAKDKIDLHSIVPIGKDPHEYEPLPEDVQKTSKADLIFYNGVNLETG
                     GNAWFTKLVKNANKEENKDYFAASDGIDVIYLEGQSEKGKEDPHAWLNLENGIIYAKN
                     IEKQLAEKDPDNKKFYKENLDKYIEKLDSLDKEAKSKFASIPNDKKMIVTSEGCFKYF
                     SKAYNVPSAYIWEINTEEEGTPDQIKHLVEKLRTTKVPSLFVESSVDDRPMKTVSKDT
                     NIPIYSTIFTDSIAEKGQDGDSYYAMMKWNLDKIAEGLSK"
     gene            complement(16959..17801)
                     /locus_tag="ACG842_000282"
     CDS             complement(16959..17801)
                     /locus_tag="ACG842_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease subunit"
                     /protein_id="HFQ0246852.1"
                     /translation="MLQNFINGLYDFHFLQNALITSVVIGIVSGTVGCFIILRGMSLM
                     GDAISHAVLPGVAISYILGINFFIGAIVFGLLASIIITFIKSNSVIKGDTAIGITFSS
                     FLALGVILIGVANSSTDLFHILFGNILAVQDIDKWITIGVSVLVLVIVVLFFKELLIT
                     SFDPLMAKAIGMNVSFYHYLLMVLLTLVAVTAMQSVGTILIVAMLITPAATAYLYANS
                     LKTMIFISATVGASSSLLGLFIGYSFNIAAGSSIVLTAAFLFVISFLISPKQKFLRKK
                     ERSL"
     gene            complement(17803..18522)
                     /locus_tag="ACG842_000283"
     CDS             complement(17803..18522)
                     /locus_tag="ACG842_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HFQ0246853.1"
                     /translation="MLEVKKLNVCYNDFLALNEISLKIQEGALTGIIGPNGAGKSTLL
                     KAMLNIIPHQGEVSINNEDINKKLVKIAYVEQKADIDFTFPIKVKECVSMGTYAGMKV
                     FQRIKNAEWQRVSKALEKVDMGKYSNHQIGELSGGQFQRVLLARCLAQNADFIFLDEP
                     FVGIDLVSERIIMDTLKKLKHQGKTVLIVHHDLSKVKKYFDNIILLNRNLIAHGSVES
                     VFNEENLKKAYGDTIFIGEGE"
     gene            19070..19360
                     /locus_tag="ACG842_000284"
     CDS             19070..19360
                     /locus_tag="ACG842_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003013637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0246854.1"
                     /translation="MTIAANNDTSVRLAVNWGEVKFLCAYPKQFKNFITTILGDDFIE
                     SRVYLNKKQTCAFLGIDNNTLDKWIRIYNFPVIRVGGVVRFDVEDIKDFMNT"
     gene            19432..20658
                     /locus_tag="ACG842_000285"
     CDS             19432..20658
                     /locus_tag="ACG842_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFQ0246855.1"
                     /translation="MPTKLSNGKYKTNLRYPKRFKEITGIASEKYQKTFPNRQLAIKA
                     ENDMKKKIEKVLREENANSLELKGKITFKKFYESKWLPRYELGQTIRSNRPPSDITIS
                     NTKDIFRLHILPMFGEYAMNYLNINTEIISDELTKKSKEYANIKIIKGYVRSMFDIAE
                     ILNYIEFNRTTKIIQSITAPKKNALEEKRIQEGKQALSSKELTNWIEAVNDDFNNHLL
                     TFHDYTLFMITLYLGDRKSETYALQWKYIDFEKQTVRLKHTLDKYQRKKFTKGRKDTV
                     IQVPEVVMTLLSEWKSVQADQLLKLKIKQTLDQYLFTYTKPSGEVNCPVHADYLNYRI
                     NSIKRRHPDLVHLSPHKLRHTYATIARQGGADMNQISNALTHSDISTTKIYVNTPDIV
                     DKAVFEAFQRGLNKCD"
     gene            complement(20746..21138)
                     /gene="rpsI"
                     /locus_tag="ACG842_000286"
     CDS             complement(20746..21138)
                     /gene="rpsI"
                     /locus_tag="ACG842_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HFQ0246856.1"
                     /translation="MAQVQYIGTGRRKNAVARVRLVPGTGKITVNKKDVEEYIPHADL
                     REVINQPFAVTETKGAYDVFVNVNGGGYAGQSGAIRHGIARALLEVDPDFRLALKRAG
                     LLTRDARMVERKKPGLKKARKASQFSKR"
     gene            complement(21154..21582)
                     /gene="rplM"
                     /locus_tag="ACG842_000287"
     CDS             complement(21154..21582)
                     /gene="rplM"
                     /locus_tag="ACG842_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003083327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HFQ0246857.1"
                     /translation="MAKPGEVERKWYVVDATDVPLGRLSTVVASVLRGKNKPTFTPHL
                     DTGDFVIVINADKVKLTGKKATDKIYYRHSNYPGGLKSITAGELRAKNSRRLIETSVK
                     GMLPKNTLGRKQFTKLNVYGGAEHPHAAQQPEVLDITNLI"
     gene            complement(22116..22583)
                     /locus_tag="ACG842_000288"
     CDS             complement(22116..22583)
                     /locus_tag="ACG842_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HFQ0246858.1"
                     /translation="MKFQQTKEVLNQLVADLSQMSVVVHQTHWYMRGPGFLTLHPMMD
                     EFMDDLNAQLDEVSERLITLDGSPYSTLREFADNTKIPDEIGRWDRTIPERLETLVAG
                     YRYLADLYQKGIEVSGEEGDDPTQDIFIEFKTATEKRIWMIQAHLGKAPEIDA"
     gene            complement(22703..24241)
                     /locus_tag="ACG842_000289"
     CDS             complement(22703..24241)
                     /locus_tag="ACG842_000289"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046316 - gluconokinase activity [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA];
                     GO_process: GO:0046177 - D-gluconate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HFQ0246859.1"
                     /translation="MNVSIGLDIGTTQTKAVAFTDEAQEAASAYFRYPLIQETQGMAE
                     QDQELIFQGVCTVIKEVIKQLPSDTIIQFVSFSSAMHGLILLDEEKTPLSRMITWADN
                     RAADEAEKLKYTVLGKEFYRQTGTPIHPMTPLNKIKWLQNSQPELFKKTAYFVGIKDY
                     IFYRLFGELISDYGTASGTGYFDIHEFQWAEEVLDYLNIRIEQLPQVFPSTYQVTGLP
                     SKTAAELGLPNDISFVLGGADGPLSNLGLGAFGDTIAALTVGTSGALRFITTQPQFHP
                     EMETFCYVLDQKHWVVGGATSNGAGIFDWAGHTFLQEVVQSAKEKGENPYDALLSAIA
                     FVPAGANGLIFHPYLLGERAPLWDADAFGSFIGLQRQHTEKEMMKAILEGICLNLYRI
                     LKGVCQENQLTEIRATGGFAQSPIFRQMMADVLGYPLVFPTVTEASALGAVILGWRSI
                     GKIQSISEAQELIELKEQVGVQETDHKKYQKIYPLFVSTQKHLAQTYQQFAQLREELA
                     DSEK"
     gene            24321..25007
                     /locus_tag="ACG842_000290"
     CDS             24321..25007
                     /locus_tag="ACG842_000290"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HFQ0246860.1"
                     /translation="MLLYFIIGCCIGSFLCLTAQRIPLGHSIIYPRSHCVKCCRSLSW
                     YELIPILSIIVQRFRCRYCRCRLPFYYLLAEALCGGLFAWFFTFSSARNFSTFIWMLS
                     ALLFSLMDLFYFMVHSHTLFCSWAILWIFWLQAGVFQWQSVLLTFIVGGVCLRYGQSY
                     LGAGDTLLMLSWSGGLSLEELLQILFLASLFGISVFSVYFICHKKKLEKLPFIPFLSS
                     ALLIVLHLRY"
     gene            complement(25208..25735)
                     /gene="msrA"
                     /locus_tag="ACG842_000291"
     CDS             complement(25208..25735)
                     /gene="msrA"
                     /locus_tag="ACG842_000291"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HFQ0246861.1"
                     /translation="MTKKAVFAGGCFWCMVKPFEEQPGIISVTSGYTGGHVPNPTYEQ
                     VTTGTTGHTEAVEIEYDPEVISYDQLVEIYWQQTDPTDALGQFADRGDSYRPVIYYND
                     EEERNIAERSKQALQDSGRFDKPIVTKIEPRSEFYPAEDYHQDYYKKNKLHYNLYREG
                     SGRAGFLRRHWGVKK"
     gene            complement(25819..26496)
                     /gene="pgmB"
                     /locus_tag="ACG842_000292"
     CDS             complement(25819..26496)
                     /gene="pgmB"
                     /locus_tag="ACG842_000292"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008801 - beta-phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HFQ0246862.1"
                     /translation="MKIKAALFDLDGVLVDTARYHYEAWLVLANQLSIPFTEKENEQL
                     KGISRTESLERLLSFGKMEQKFSEKEKSAFAEQKNNLYLQAIQKMDETSVLPGAIAVL
                     EYLKKTNIKIGLGSASKNARLILEKTNLTSYFDVLIDGTQVSKAKPDPEVFLKGAQQL
                     NVPPNACLVIEDSEAGCQAALAGNMHVLGIGENINLPSAEYVIPDLTVFDQVRSFWHL
                     SEAVSRI"
     gene            complement(26493..27623)
                     /locus_tag="ACG842_000293"
     CDS             complement(26493..27623)
                     /locus_tag="ACG842_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HFQ0246863.1"
                     /translation="MRILIAPDSFKESLTALEAAQAIQSGFQEVFVQAEYDLLPIGDG
                     GEGTVSSLADSLRLKRQTAVVTNAFGKNKEIQYASDGQTAIFEMAEIVGLKDVPLNQQ
                     QPLRLSTRGVGELIRQLLQQGVRHIIIGVGGSSTNDGGIGMAEGLGYAFYDQDNQKVE
                     ALGENLGKIATVDKTNVPIELIKAKITVITDVTNLLCGKEGASFVFGPQKGLKENELA
                     VADQAMHDFYMRFAPQVIEASGSSAGGGMGAGLLAFASGKIVSGIDYVLDLLEFDKRV
                     QQADLVIVGEGRLDGQSLKGKAPVGIARRVPEGVPVIAICGSVGKGSEKAADVGISAV
                     FPILPAAVPLQEALQQATDNLHRTAKNIASLLQVVHFQGGNR"
     gene            complement(27623..29806)
                     /locus_tag="ACG842_000294"
     CDS             complement(27623..29806)
                     /locus_tag="ACG842_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 65 protein"
                     /protein_id="HFQ0246864.1"
                     /translation="MKVEEKQFDLEYINKYASQMTIGNGFFGIRGSQEEDYRNQVRGM
                     FAAGVYNRPLGAESAELVNLPDVIRWEIKIDGEHFSLETMKVLAYERALDLEMGELKR
                     YALIETAQGKKVEVITCRVADQNNLHQAALKVVIKPLTEDCQVDVKTGIDGQQTNFGT
                     QQLVEKELRVFGDDLISASYQTTESKIDIGIAAKFNKAGVFQAKNRRITASFSEKIRK
                     NTSFTLEKIIVLSTSLQQTDPFKKSIAQAQKSVSYTEILEQSMSYWTDFWHQHRITIQ
                     GDSSFDQLAMDFAAYHLQIMTPRNDPSVSVGAKGLTGEGYKGHVFWDTEIFILPFFLY
                     QFPEVAKNLLLYRYDRLEGARQKARDYHYQGALFPWESAHTGQEETPKFAALNIKTGT
                     RQEVASAAAEHHISADLAYAVQEYVQATEDQSFLETKGKTLIHETAAFWLSRAVKVDD
                     HLEIHRVIGPDEYTEYIDNNAYTNYLAHYNVALAVKYQAGDPLFLKECQNFLDKLYLP
                     VPNKEQLIPQDDTYLSKPVIDLTTYKERPGSQSILLDYARSEVNELQVSKQADLVMLL
                     YLLPEYFSQPIVSKNLHYYENKTIHDSSLSKAIHAIEACRIQEMEWAYHLFQEACLID
                     LGPDPHSSDDGIHAASLGALWLAVIFGFSGITVTNGTLSISPRLPSAWEKISFPFIWK
                     NHRLLFVLTKKQIVIKKETKETLPIFVNGDQYQLEKELILERRGK"
     gene            complement(29803..30828)
                     /locus_tag="ACG842_000295"
     CDS             complement(29803..30828)
                     /locus_tag="ACG842_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFQ0246865.1"
                     /translation="MKKVAIIGAGQVAEKVHAAYYQKRSDVLELAAVVDPVSQRAEDF
                     CRRTGFSHAYTSVTQMLNEISPDIVSVCTPNRFHFEHVLAALEANASVMCEKPPAMTA
                     DQARKMAETADKNGQILAYGFQHRFSDEATMIKNHLDVLGDIYYVEANALRRSGVPGW
                     GTFIDKELQGGGPLIDYGIHMLDTAMYITGFPKVKSVRAYSFQKIGTKKNSGKFGKWD
                     PSKFTVEDAIFGTLELENGKIIQLNTSFALNIREDKLNVAFCGDQAGANLYPGELFED
                     VDGKLVSLQKTTPVNDPHELSMAHFVNRALGDQTQMIADGWQGYVIQNLVENLYRSAE
                     TGEVITL"
     gene            complement(30873..31700)
                     /locus_tag="ACG842_000296"
     CDS             complement(30873..31700)
                     /locus_tag="ACG842_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HFQ0246866.1"
                     /translation="MKFGTQDQAFFPRDILEKFRFIKDCGFEEYEIDGKLLIENIDTV
                     KQAIKETGIPVVTACNGYDGWIGDFIEERRLNGVRQIAEILRALSEVGGKGIVIPAAW
                     GMFTYRLPPMVSPRSKEGDYKAVSESLRYLDKVAEETGTVIYLEPLNRYQDHMINLLA
                     DARHYIDSNQLKHVQIIADFYHMNIEEDDISKALTTQKEYVGHIHLADNHRYQPGSGA
                     IDFTRHFQTLLQNEYEGYMVYECRLRGENEVKIYQESLQYLKNCLETARHLNDLEKE"
     gene            complement(31697..32764)
                     /locus_tag="ACG842_000297"
     CDS             complement(31697..32764)
                     /locus_tag="ACG842_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HFQ0246867.1"
                     /translation="MKKLVALEPRIAGFKDYEDREIEKNEVRIRVIFATPKPGTEIVD
                     FRGLSPFIDEEFSEEWRLFLPREEGSQRGVVFGEFQLGNMIVGTIEETGSLVTEYQKG
                     ELVCTYGPIRETLITNGVDNYKLRKLDRLDQWKNAVCYDPAQFALGGIREAHVRAGDA
                     VAIFGLGAIGQIAIQLAKKAGATTIVGIDPIAQRRELAEKYGADYTLDPMKADVGFRL
                     KELTDKRGVDSVIETSGSAAALQASLRGIGYGGIISYVAFAKPFPAGFNLGREAHFNN
                     AKIVFARASSEPNPDYPRWNRKRIEDTCWELLMNGYLDCEEIIDPVVPFAESAEAYMR
                     YVDQHPEESIKMGVQVRGGEQ"
     gene            complement(32790..33626)
                     /locus_tag="ACG842_000298"
     CDS             complement(32790..33626)
                     /locus_tag="ACG842_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0246868.1"
                     /translation="MKNKKSRKILFYATILLLLAVNLFPFLIMLMTSFKDSQEAIATT
                     QTIFPKEWTFEHYQDIFNAEIFPFIMYFKNSLVISLIASVTAVVIGISGAYALSKLRF
                     KARSKINASFYTVYMFSGILIVVPLFKIISGLGLYDTNTALIITMVVQTLPTAIFMLK
                     SYFDTIPTDIEEAAMIDGLSRIQIIIRIIIPLAISGIISVFVYCFMVSWNDYLFASIF
                     LSTSEKFTLPIGLNTLFSTPDYIWGRMMAASLVTALPVIIMYALSERFIKGDLVEGGV
                     KG"
     gene            complement(33623..34492)
                     /locus_tag="ACG842_000299"
     CDS             complement(33623..34492)
                     /locus_tag="ACG842_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0246869.1"
                     /translation="MNRPRKHSDLKLAMGLLAPSMLLLAVLVLIPMLSNIQISFFDMP
                     LNPNSDAVFIGLSNYLEILSDSEFYRSLGLTVAYTFLVVAGSTALGLAVAILLNRKFR
                     FRKTVRSLILLSYVTPSISLIFAWKYMFNNSYGIVNDLLVDRLQLFNEAPLWFDQPVA
                     SFFMVVLFAIWRYYPYAFISFLAILQTVDKTLYEAAEMDGANAWQKFKIVTLPAIKPV
                     LATVIVLRSIWMFYMFTDVYLLTNKVNILGVYLYQTAFAFNDLGKAAAISILLFIIIA
                     AVVLLSRKKVDLK"
     gene            complement(34562..35845)
                     /locus_tag="ACG842_000300"
     CDS             complement(34562..35845)
                     /locus_tag="ACG842_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFQ0246870.1"
                     /translation="MKKIIVTTCLLATGFLLTACGESQSSNESQQTTIQFMHSSVEQE
                     RLSVINELVADFEKENPDIKIKQVPVEESAFNTKIVTLARSGKLPEVMEVSQDFAKVM
                     DKDELIDQTAVQNVIEEAGEDNYYDGAKNLVRSEDGKSYIAAPISGWVQGIWYDKEKL
                     SEAGFSEPENWDDILKIAQHFTDKENKQYGIAMPTAEGTMSEQAFSQFALSNGANVLD
                     DEGEVTIDTEKMREALSFYQNLSQYTMPGSNDVTEIKDAFMNGTAPMAIYSTYILPSV
                     YEAGNSENIGFAIPTQKDQAVYGTVSGLTISSGLDEKQKEAAEAFTAFLSQPKNMEKW
                     VLMSPGGAQPVNKQVVELDGYKENEVIKSFGELPSEIASAFDEVQVFGLVGEKNFTKM
                     GDITSSGVIGKAVNQVTVGNEDVDQALADAQKNTK"
     gene            complement(35859..37544)
                     /locus_tag="ACG842_000301"
     CDS             complement(35859..37544)
                     /locus_tag="ACG842_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HFQ0246871.1"
                     /translation="MYTKLLQAIYRDSDLSEKTASMIEQKVSVYQQKKGQAGKLHWDE
                     EDIFLITYGDQFFEEQQPTLRTFKKFYQKYLADTFRVVHFLPFFPYTSDDGFSVVNYQ
                     AINDRLGTWEDIEEMRSETRLMFDFVCNHMSAESTWFQEYLNLNDTYKDFFIDLPLDT
                     DTSSVTRPRTSPLLTTFIDKNGKEKHVWTTFSEDQVDLNFKNPEVLLKMIDILLFYLE
                     KGASFIRLDAVGFLWKELGTSCIHLPETHKIIQLFRAIADEAAPGTVLITETNVPHKD
                     NISYFGNGENEAQMVYQFSLPPLTLQAIRSGNTSKLKEWLKTIDFASKKTTFFNFLAS
                     HDGIGLNPVRGILPEKTILELVEQLKKEGTRVNYKKNPDGTTSPYEINATYLDALSSP
                     TDTTEWRSKRFLAAHSFLVTLPGVPAIYIQSVLGSRNDYKGVEKTGENRKINRQKFLL
                     ADIEKELQTDPLRTAVYGELTQMLSIRKKEKAFHPNSLIYLLDSADSFISFIREAKEE
                     KVLVIHNFSNQSAGYLLPKGDYLDLFSGQIIHCSQERKLIFQPYEYMWLKIIK"
     gene            37819..38844
                     /locus_tag="ACG842_000302"
     CDS             37819..38844
                     /locus_tag="ACG842_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFQ0246872.1"
                     /translation="MKPTIYDIAKKANVSKSTVSRVLNNQPNISDKAKASVLQAIKEL
                     NYEPSKIARGLSGNGFDAILALSHRTSHTTYGNPFFSDVIQSISAVSEQNNFDLILQT
                     AKTPEEELEKCAKKIQEKMIRGIIILSSTMDETLLFELDQYNIPIVVIGKVGNDYTNI
                     YSVDTDNFQDSYDLVETLIKNGHRHIGCLHAPTEVYVSNDRLNGYRQCLFDHHLDIRN
                     EWIIDGGFRLEDSMAAMNDLMKLRERPTAIFSTDALKSLSIYKVAEQSGKQIPDDLSI
                     AGFSDPLLSSLFSPQLTRIDIPTNQLGIKATELLFQLIHHQYPEEKRIILSTEITLTD
                     SIQQQEM"
     gene            complement(39439..43092)
                     /gene="rpoC"
                     /locus_tag="ACG842_000303"
     CDS             complement(39439..43092)
                     /gene="rpoC"
                     /locus_tag="ACG842_000303"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HFQ0246873.1"
                     /translation="MIDVNKFESMQIGLASPEKIRSWSYGEVKKPETINYRTLKPERE
                     GLFCERIFGPTKDWECACGKYKRIRYKGIVCDRCGVEVTRSKVRRERMGHIELAAPVS
                     HIWYFKGIPSRMGLVLDMSPRALEEIIYFASYVVIEPGNTNLEKKQLLTEREYREKRE
                     QYGQEFQAAMGAEAIKQLLDNVDLDAEAAELKEELKSAQGQKRTRAIRRLDILEAFRA
                     SGNQPSWMVMDVIPVIPPDLRPMVQLEGGRFATSDLNDLYRRVINRNNRLKRLLDLNA
                     PGIIVQNEKRMLQEAVDALIDNGRRGRPVTGPGNRPLKSLSHMLKGKQGRFRQNLLGK
                     RVDYSGRSVIVVGPFLKMYQCGLPKEMAIELFKPFVMRELVQRELASNIKNAKRKIER
                     QEDEVWDVLEDVIKEHPVLLNRAPTLHRLGIQAFEPVLVQGRAIRLHPLVCEAYNADF
                     DGDQMAVHVPLNEEAQAEARMLMLAAQNILNPKDGKPVVTPSQDMVLGNYYLTMEEEG
                     REGEGMVFRDQNEAVIAWRNGYVHLHSRIGVNPNSLGEKPFTEWQKERTMITTVGKII
                     FNEIMPPEFPYLNEPTDFNLTVQTPDKYFVEAGTDIPAHIKEQELVLPFKKKNLGNII
                     AEVFKRFKVTETSKMLDRMKDLGYKHSTHAGITVGIADISVLNEKQEIIENAHKQVET
                     ITKQFRRGLITDDERYERVIAVWNAAKDSIQQKLMEGLEAKNPIFMMSDSGARGNISN
                     FTQLAGMRGLMAAPNGRIMELPIISNFREGLSVLEMFISTHGARKGMTDTALKTADSG
                     YLTRRLVDVAQDVIIREEDCGTDRGLEIQAIREGNEIIESLEDRLVGRYTRKEVRHPE
                     TNELIIAGNQLISEDIAKQIVDAGVETVTIRSVFTCNTKHGVCKHCYGRNLATGSDVE
                     VGEAVGTIAAQSIGEPGTQLTMRTFHTGGVAGDDITQGLPRVQEIFEARNPKGQAVIT
                     EVTGDVIDISEDPATRTKEVTIKGKTDTRTYTVPYTARMKVAEGDMIHRGAPLTEGSI
                     DPKQLLQVRDVLSVENYLLREVQRVYRMQGVEIGDKHIEVMVRQMLRKVRVMDPGDTD
                     ILPGTLLDIADFKDQNYNTLVSGGVPATSRPVLLGITKASLETNSFLSAASFQETTRV
                     LTDAAIRGKKDPLLGLKENVIIGKIIPAGTGMQRYRDMEPKEVGVASENVYSISDIEA
                     QMAAEDAMNNQTK"
     gene            complement(43171..46788)
                     /gene="rpoB"
                     /locus_tag="ACG842_000304"
     CDS             complement(43171..46788)
                     /gene="rpoB"
                     /locus_tag="ACG842_000304"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HFQ0246874.1"
                     /translation="MAGHVVKYGKHRERRSFARISEVLELPNLIEIQTDSYQWFLDEG
                     LREMFEDILPIDDFNGNLSLEFVDYELKEPKYTVAEARAHDANYSAPLHVTLRLTNRE
                     TGEIKAQEVFFGDFPLMTEQGTFIINGAERVIVSQLVRSPGVYFHGKVDKNGKEGFGS
                     TVIPNRGAWLEMETDAKDISYVRIDRTRKIPLTVLVRALGFGSDDTIFEIFGDSETLR
                     NTVEKDLHKNASDSRTEEGLKDVYERLRPGEPKTADSSRNLLNARFFDPKRYDLANVG
                     RYKVNKKLDLKTRLLNLTLAETLVDPETGEIIVEKGTVLTHQVMETLAPFIDNGLNSV
                     TYYPSEDGVVTDPMTVQVIKVFSPRDPEREVNVIGNGYPEAAVKTVRPADIIASMSYF
                     LNLMEGIGNVDDIDHLGNRRIRSVGELLQNQFRIGLARMERVVRERMSIQDTETLTPQ
                     QLINIRPVVASIKEFFGSSQLSQFMDQTNPLGELTHKRRLSALGPGGLTRDRAGYEVR
                     DVHYSHYGRMCPIETPEGPNIGLINSLSSYAKVNKFGFIETPYRRVDRETGRVTDQID
                     YLTADIEDHYIVAQANSPLNEDGTFAQDVVMARAQSENLEVSIDKVDYMDVSPKQVVA
                     VATACIPFLENDDSNRALMGANMQRQAVPLINPQAPWVGTGMEYKSAHDSGAALLCKH
                     DGVVEYVDASEIRVRRDNGALDKYDVTKFRRSNSGTSYNQRPIVHLGEKVEKGDTLAD
                     GPSMEQGEMALGQNVLVGFMTWEGYNYEDAIIMSRRLVKDDVYTSIHIEEYESEARDT
                     KLGPEEITREIPNVGEDALKDLDEMGIIRIGAEVQDGDLLVGKVTPKGVTELSAEERL
                     LHAIFGEKAREVRDTSLRVPHGGGGIVHDVKIFTREAGDELSPGVNMLVRVYIVQKRK
                     IHEGDKMAGRHGNKGVVSRIMPEEDMPFLPDGTPIDIMLNPLGVPSRMNIGQVLELHL
                     GMAARQLGIHVATPVFDGASDEDVWETVREAGMASDAKTVLYDGRTGEPFDGRVSVGV
                     MYMIKLAHMVDDKLHARSIGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGAAYTL
                     QEILTYKSDDVVGRVKTYEAIVKGEPIPKPGVPESFRVLVKELQSLGLDMRVLDIEET
                     EIELRDMDDEDDDLITVDALTKFAEQQTAKELEKKAAEQVEDEKDDVIQNFETAEDNL
                     D"
     gene            47516..48484
                     /locus_tag="ACG842_000305"
     CDS             47516..48484
                     /locus_tag="ACG842_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFQ0246875.1"
                     /translation="MKKNIIIGLGAALLLALGGCAQKDNSAEASDGQKTIGVLQLVQH
                     NSLDAAYKGFKEGLAEEGYKEGDNLTIDYQNAQNNQDNLKSMSEKLVKEDPDLLLGIA
                     TPAAQSLLNETTTIPMTVTAVTDLEQAHLVTSNEKPGGNVTGTTDMVPIEKQIGLLLN
                     IVPDAKTIGIMYNNGEANSKIQADLAEKELKKAGVKVKVLTANSTNDVQQVTKSLAKD
                     VDGIYIPTDNTFASAASVVGEVAKETKTPVVAGAVDQVKDGGLATVGIDYESLGKQTG
                     IMAAKILDGKNPSELPVETANDLELYVNKDMAKALGINPDSIKEAE"
     gene            48502..49407
                     /locus_tag="ACG842_000306"
     CDS             48502..49407
                     /locus_tag="ACG842_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0246876.1"
                     /translation="MTNLLSLFLSSASQGVLWALLAIGVYVTFRILDIADLTAEGSFP
                     LGAGIAAVSITNGYHPLTACLLGFLGGAAAGLVSGLLHTKLKIPALLAGIITMTGLYS
                     VTSRVMGAPNISLLGQPTIFHWVESFGITKDNAVLIVGLLFALIVVCLLVLFLRTETG
                     LAIRATGDNLAMSEANGINTDNMKIIGYMISNGCIALSGSLLAQNNGFADLNSGIGTI
                     VIGLASIIIAEVLLRNQPLFLRLLTVVLGAVIYRFILALVFELNVEPSDSKLASAFVL
                     VICLSFPQIQQKFHLPQKKKGGSVK"
     gene            49404..50210
                     /locus_tag="ACG842_000307"
     CDS             49404..50210
                     /locus_tag="ACG842_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0246877.1"
                     /translation="MNDVLTLENLHHTFEKGTINENHVLKGINLSLDPGDFVTIIGGN
                     GAGKSTLLNSIAGTFSVDQGQILLNGKNITKKSVVERSKSISRVFQDPKLGTAVRLTV
                     EENLALAMKRGKKRGFFRGVKPQDRTFFKDQLVRLNLGLENRLSSEIGLLSGGQRQAI
                     TLLMATLQRPELILLDEHTAALDPATSKTVMELTEKLITEQNLTAFMVTHDMEDAIRY
                     GSRLIMLHQGRIVVDIQQEEKQQLTVPQLLALFKQNSGTALKDDQVLLSS"
     gene            50367..51335
                     /locus_tag="ACG842_000308"
     CDS             50367..51335
                     /locus_tag="ACG842_000308"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HFQ0246878.1"
                     /translation="MTTKDKVLQLLKKTPDFLSGEKLAQQLEVSRTSIWKAIKELEKA
                     GYRFEHGPTGYRYLPSDVLDAKEIQQELRSFIPDLKVQTMLTSESTMKDVKLAAAQGD
                     SVSKLFVADTQEQARGRFGRPFFAEPGRGIYMSLLLHPDKRFDELPQYTIIAASACVK
                     AIESVTGRSASIKWVNDIYMDGKKVCGILSEAVSDMESGRISHIIIGIGLNFSVPQNH
                     FPADLQEKAISVFPDGQASVTRNQLISKIWQELFTYLSHPEKALDFYKEKSFVLGKTV
                     SFNQKGTTYVGLAKEIGDHGELIVQTNEQKMSLSSGEISLTSIRTQ"
     gene            complement(51652..52176)
                     /locus_tag="ACG842_000309"
     CDS             complement(51652..52176)
                     /locus_tag="ACG842_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF664 domain-containing protein"
                     /protein_id="HFQ0246879.1"
                     /translation="MDYLDMLCESVDRAYDRFERLFEGVSIEEANRFPVEDRAPQIKS
                     MTWLAWHTARELDFQIADLAGTSPIWHSQDWESRFPMTIADNEQDWKHSLEQAKQIQA
                     NQIEDLLGYLRGAADFAISYLRRLEPSELDEVIDENWTPPVTRGMRLVSIIDDAAMHS
                     GQVIYARRLLGLRD"
     gene            52463..>53655
                     /gene="scm"
                     /locus_tag="ACG842_000310"
     CDS             52463..>53655
                     /gene="scm"
                     /locus_tag="ACG842_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HFQ0246880.1"
                     /translation="MKKRASLLVLVALLFNIFSYGVAVSAETYIPKDPGNIYNDLTGN
                     YGLLGIASQFHVFAKNKTTVNAHTDGNIATHELDAKNNFGTELHEGLLNQEINYIQKT
                     DSFANSSGIPTGSTRMNKFVVGSNVDVALQDGHPTVNGSRLDHLTPDDFYKDRAGHEY
                     IDFEQEFAKLNVAANDLATITPAKTYTAADFPDMNNRTIDLTDLSDTGFLLVNIDAEV
                     LTMNTPLQIINPNDQVVVFNVINSSSALNVQSPIKYNDRSNHETEDFSDANISWNFGN
                     EMTDLTISAPFQGTILAPNATIRITQNQDGTIIGTNVILDAATNRWDPNEIFISDNGT
                     DTTDTSDSTDTSTTSDSSDSSTSSDSTDTTSTTSDSTDTSASSDSTDTTSTTSDSTDT
                     SASSD"
BASE COUNT        16058 a        10953 c         9157 g        17487 t
ORIGIN      
        1 tttctttgag ccagctgtta ccagaagtgg tgtgtttcga tgaattcaaa tcagtgaaaa
       61 atgtctcggg agccatgagc tttgtcatga tggacggcaa gacccatcaa ctgattgata
      121 ttgtggaaaa tcggcaactc aatcctttac gcgactattt cttgcgctac cctataaaag
      181 tccgtgaaca agtgcgtcta gtggtttcag atttctatac accctatcgc acattggtga
      241 aagagtgttt tccgaatgcc cgaatcgtcg ccgatcgctt tcatattagc caacacattg
      301 gtcgggcttt taccaaccac cgaattcaag taatgaagac ctttaaaaaa ggggatcgtc
      361 ggtcgaagca cttaaagaaa tactggaaac tacttcaaaa gaatgcgtgg gaattgaaag
      421 gtcagcatcg ctactggcgt ccgtcatttc gagatcatct gacagaagca gagatagtcg
      481 atcggctatt atcttatgat gattccttaa agcgaggcta cgaagtctac caggattttc
      541 tttccgttat cagaagacaa gatgttcctg aattcgtcgc gctcctaaaa gaagactaca
      601 aggagttgcc agagcactac caaccagtgt tcacgacctt caagaaatac caaacagaaa
      661 tcaaacgagc gttgcgcgtt ccctattcca acggccctat agaatgtttg aataaccata
      721 tcaaagtact aaaacgaatt gcctatggct atagaaactt tcaaaactac cgagaacgaa
      781 tctttttata tcgaggaaaa tattttaaga aaactaagaa tactacccaa ctgactaaag
      841 ccagaacaac aacacggcta gacaagatag ctgtctagcc gtgtgagtat ctcgattatt
      901 ctattgaatt tcttcaccaa cactcattga cgaagagcca aaataagtaa tctaaccgac
      961 tgttaactaa accattgcag gtcccgttaa agatacttgt gcctgttgtc accgcttctt
     1021 tttcttgtcc acaagcgctt aacacactta ataaagcccc tgctattatt aataataata
     1081 tctttttctt attaaaaacc tcctcatcta tttccagatt agtaaatgta gtaatctgtg
     1141 ctttcttttt tctcaatact aataactaac ttatggggta aacaaatact cgtttgaccg
     1201 ggttttgaga tccatccagt tctaaccgcg atttgatcag gactattatc ttctttaact
     1261 cgaattcttc catctttaat ctcaacaata ttatattgtc ccttcgcagg atgataattc
     1321 acgagtttat gattgatctt atccaaatcg aagcgatcca tttccttgcc atcaattcga
     1381 acgatggcat atttgatagc atcttcagtt gtttcattct ccttttctat agagaataca
     1441 aataagggaa taaaagaggt aataatcaaa atgccaataa ttatgacatc gcctatcttg
     1501 acaccagctt ttcttaaaaa tttcattaac gatatctggc taaacatatt agactttttc
     1561 catggaaatg gtgaaatttt tcggaaatta tctgcattaa gagaatcagt taaacaaccc
     1621 tcaagttgtt ccttaataca ttgttgatcc atcttcatac caataaaaac tagtttatta
     1681 atgcgttcac caaacaacgg atgccaggct tccttcaatt cgggattttt ttgtataatc
     1741 atttgacact caattttagg taaactagct acccaataat ttaatgattc aatactttta
     1801 tagctacccg atgtttcaaa gatcgtcgaa aaatcactat gttgggcaaa ccaaagaaac
     1861 ccttttgctc ttgtaatatt gccaggaaaa tttttatccg taagatgggc aaatttttgt
     1921 gcgtcaagga gtaaccgatt tcgatagata aaacttgaga taccatattc ttctgtttca
     1981 ggaatatgat tttgatagcc aagctctaac tctttgaacc agcccactga ataggaagtc
     2041 tcatctatat taaaaagatt tgtatttaga attttcgata agtctacttc acctttaatt
     2101 gtcctaatta ttttggcatt ggactgtaac ttagcaatta gaccgttaat tttgtaaatc
     2161 gtcttggttg acactaagtc actattattt aaaagtaata tgttacaaca ctcgaattga
     2221 tcaattaaca agccacgaat atcctgttct tcttcaacta cattaccttc agcttttctt
     2281 tcctgaatct tttcacccgt ttcgaaatta tcccaaaaac gttttacatc cactactata
     2341 ataatggcac caatctctaa tcgttgggtc aaatcaatgt ctaaagggtt tttagctaat
     2401 ataattgctt gaataattgg aattggtttg ctgattcctg aagcttcaat aacaatttga
     2461 ttcaagtcag aaaattgcac taattgatct agttcaacaa tcaaatcttc gcgtaatgta
     2521 caacaaatgc atccattcgg catcgaaaca agtttttctc cagttctttt aaaacctttc
     2581 ttgtcgacta ataccccatc aacatttact tcccctatgt catttacaat gacagcaatt
     2641 tcctttccgt gatcaccgta cagaatatga tttagaagta tcgtttttcc tgaaccaaga
     2701 tagccagtta agattgtaat aggtattttt ttcataggga acctctttct ataccttgaa
     2761 tttacacttt aagtgcaaca cataaaaatg agtcataatc gatatccaac tagtataagt
     2821 ttgtcgagaa cactcctaga aggaaaataa ttatgagcca gtatcgtcat cttactatac
     2881 tcgaacgaga aagaatcttt ctgcttcacg aagaaggatt ttctattagt ataattggca
     2941 acgacattca gcatagcccg tttactattt tacatgaact gagaagaaac aagaaaaaaa
     3001 ctattcacct tccaaagcgg aaaaaaatta tgcttatcga agaaaaaaat gcggacgtta
     3061 tcacattcta aatgatccgt gagtttggtc aattgttcgt catttatttg tcgattttca
     3121 gtggtcgcca gaggaaatct cgaatcgttt gaagcatgaa catatggact tttcaattag
     3181 ttttatcacg atctaacgtg caatttgcaa tgatcttttc gatacagaac ccctctcaca
     3241 cggcaataaa ggcctgatcc gttcattgca ccatcgagga aaaacaagac atacaaaaaa
     3301 tcatgtcgag tctcgtggaa agatttgcct tactcattcg atccatggcc gtccaaaatc
     3361 cgcagatgat agaagtgatt ttggtcattg gaaagccgat attgtagctg gcaaaactgg
     3421 tggtacttgt ttagtgactt tgacagacag aaaatcatgc ttccttttag caggaaagaa
     3481 ggtccccaga aagaaagccg cttatgtgag cgagaaaatg attgaacttt taagcagtat
     3541 tccaaatgag gcggtcaaaa cgatcactcc agatagagga aaagaattct ccaatcattt
     3601 cgcagttaca gaagcattga ataatatgcc cttttatttt ccagatcctc atgcccttgg
     3661 caacaatgaa caaattaaaa tacgaataga ctcattcgag aatacttgcc aaaatcgaaa
     3721 gagatagcta cggtagataa ctcaacgata aatcagatga ttgaaaaact gaataacaga
     3781 cctagaaagt gtttagattg gagaacacct tatgaagtct tctttgataa agtgttgcgc
     3841 ttgatttgac aattcaagac ataataaaaa gcagatcaaa tccaaataag ggaaaaatga
     3901 tctactctgg tattatcaat taattggcca agctattatg ccgttttgct aaacgtctac
     3961 ttcttccagt attgaccttg ataaaacaac aatcaggatc ccgttttaat actctatgaa
     4021 ctgtgtcaat actaacatgt aaagttgcag caatatcagc taatgtactc ccatttaatc
     4081 tcatttgctt tgcaaatcga taacgccgat aagcatcaat attcttggaa tccatagaca
     4141 ataatacttt tcttaccatg tatttcctcc aaaaattgaa ttttaatgag ccatcatttc
     4201 ttgagcaatt tcatgtttag acaatgaagc tggatagaaa gtaggccaat tatgcaattc
     4261 attatagagt ttttcttgac tttctccacc aaagaaaata tggaaatgct ctgccttact
     4321 tggtgcaatt ccatgatcac taaattgaat atatttaaaa cgaccagcac ctgcatcatc
     4381 agtttcaaaa ttaaaacgaa caccacggtt tcctttttca taattcaaaa ttttgtatcc
     4441 aacatatttg tatgtgtatt gatgatgttc accgttgact acaaaatcaa tggtgttgtc
     4501 tttaatgttg atagtatcaa tatctgtctt atagccagta gtataataat ctttgtactc
     4561 ggcagctgtc atgtctttat tgatttttgc tttgtaatcg aaaacttcat ccaaaattcc
     4621 atcttttaac aacggataaa cagattgcca ttcgcctgta taatcactca attcgcgatc
     4681 cttcacatcg gcatctttaa agtacccatt agcaactgtc ttttcatcct tcgcttcttt
     4741 ttctggaact acctcttcac cagcaaccat agttgttttt tctaacgctt ttagattgtc
     4801 ttccattact gaaacataat cttcaccatt atccatttgt tcttttgtaa gactttcaag
     4861 aggattcagt acttccaatt ttatcccagc ttcgttggcc aaagtttttg caattttatc
     4921 attagcattc ttttcaaagt aaatataatt gatcttattt tttttaacat actcttttaa
     4981 ttcagctaat cgaccagaag atggctcttc ttcaggactt agtccagcaa taggaacttg
     5041 tttcaatcca taatccaaag ctaaatatcc aaaggctgcg tgttgcgtca caaaactttt
     5101 ttgtttcgct tctttaagac tagtggtata gtcagcatcc aaccgtttca gtttagtcaa
     5161 atacttttca gcatttgtct caaatacttt tgcttttttc ggatacaatt taactaactg
     5221 atccttgata tttgaaactt ccttaattgc cattttgggt gaaacccacg tatgaggatc
     5281 tagttcatga tggtgatctt cttctccgtg atcatgatct tcttcttcac tacctggcaa
     5341 taaaatcatg tcttttgttc cttctactac attaggccca gctttcttcc aactttcttt
     5401 agcttttggt acccatgatt ccatgttttc gttatgatat acaaaaacat cagcatcatg
     5461 agcttttgcc atatccttag ctgagggctc atagtcatga ggttctgatc ccgctggaac
     5521 cattaaatcc acgttcgctt catcgccgac gatgtttttg gtgaaatcat acattggata
     5581 gaatgttgtt acaatttcta attttccatc tttatcattc gatacttttg catcattagt
     5641 gctggagcaa gctcccaata atcctagagc tgctatccca ataagtccta cactgatttt
     5701 cttcattctt tttccttcct taattatgct aatttcttca ttttatttcg tttatgttct
     5761 ttgataattt ttaatgccct ttttctagtt ttaatattcg ggcttttaag tcgtcgatat
     5821 gcactagata tatccgtttt ctccattaga aattaccccc aatcactact tatcattatt
     5881 tgctttccgc cagtaattta tcaaaactct gtttgatcct ttcttcatcc aacatttttc
     5941 cgattagaat aatagtggtt tggtcttttt gtcgagtggt gtattgaaaa ttaacctgtt
     6001 cgtttacctc ttgcatggca actataaaat cctgctcttg taaagcaatc aatcctttaa
     6061 aacgatacag gctcccttgg ttggttaaaa tcagccagtc aatccattgg agtaaaaaat
     6121 ctttttttat gtcttgatct gaagttaaga gaattgattt aaactcatgt acttgatggt
     6181 gttgatgata gtatttgcca ttatagtgat tttctgatga atcactttca aaaactaagg
     6241 gatgattaaa ttttttcaaa ttataaaaat cctttttttg aatcgtttca ctatgtgtga
     6301 acattcgaat atcggataaa gggttaatac ttttgatttc tctctgtaat ttttttagtg
     6361 cagataaatt caagttttgt tttctagaca caaaaatgcg atctgccatg acaagttgct
     6421 taagggcttg tggttctctt tcaataagtt caaaactgtc actatcaata acagtcaata
     6481 aactatcgat atatactctt cctcttagtt gaggcgctgt taaaagtgtt tgaatgattg
     6541 gttggggatc tgctatacct gtagtttcaa tgatgatttg tgaaattggt tgttgatgtt
     6601 cttctattgt ttccagtaaa gaaatcaata ttgctaataa atcactgcga agactgcaac
     6661 acatacatcc attatttagt tgtaacaccc gttcctcgct atgaactaat aatttgtgat
     6721 caatcccaac ttcaccaaac tcattttcaa ttataataat ttcttccctg gggtagggtg
     6781 agacttgcaa ggcttgattg attagcgttg tttttccagc ccctaaaaac ccagaaacga
     6841 ttgtaactgg tatgcccatt ttgtcacctt tttaccactt agctatttca cttctttaaa
     6901 aattgcccgt ctgcgcaatt ttggcgaata cttttttaat tctaaccgtt cagggttatt
     6961 ccgtttattt ttgctggtga gatacagacg ttctccagtt tcgacacatt ccaaaataat
     7021 attttgacgc ataaatcttc ctccttcaat acttatcgat cttcaagata tgcttgaatt
     7081 tgttgtgcat cttcaaaagt taactcgcca gattccaatt gtgaaactaa attttctttc
     7141 atatcaacag gtatctccca ataaccatcg atacattgaa ataactcctc gtcatactct
     7201 tcaaaaattg gatagattct taaaaaatca tcaaatgata agccttggtc ctcatagtaa
     7261 tctttgacta atccattttc aattgcctgt tgggtcggta aacccgtttc agtaataatt
     7321 cctctctcta ccatcgaacg ataaaaagtg catttatcta gcgattgctg ggtcgtttca
     7381 agataatact ttaatccttc ttcaactaaa ggtgggtaaa taatttccat tttgattccc
     7441 tcctaaaagt tatatattaa actattttga agctccttta attactttct ttccattata
     7501 gtaccctttt aaagatacat gatggctacg tctataatca ccattcgctt cgtcaaatga
     7561 aatttctggt tttactacag tttgatgagt tcttcttaac cgtttcttcg ccttagatgt
     7621 ttttcttgct ggtactgcca tgatatttcc tccttttacc aacttgcttt ttttactcca
     7681 ggaatcaacc cttgatgggc aagttctcta aacttaatac gggacatacc gaactttcgc
     7741 atataccctc ttggacgacc atccgtctga tcacgaagcc tcagacgatt cggatttgaa
     7801 tctcttggca atttagcgag agcggtgcga tctcctgcgg cttttaattc ctgacgttta
     7861 tctgcatact tttcaatcat cgccatttgt ttctttgctt tagcaatttt cgactttttt
     7921 gccatatacc tttatactcc ttttattttg atttagacac ctcaaatcgt aatcgttacg
     7981 ttttgacact gatactttac caacaagagt tgctcttgtc aacaataaat aacatgaaat
     8041 aaaactcata tgaatagata actatttgaa aacgaattta tcggtacata gagataatca
     8101 aatcatttaa aagaatatgc agtttaggct ctgaaaaata agaattcact tttattagtc
     8161 caatcaaaca caaataccgt tttaagtagc ttttcctcta cttaaaacgg caatatgaaa
     8221 gaaactataa ctttttattc aatcttttat gtttaactgg tttatcgcca attttttaaa
     8281 actttgatta ttccttatat tataagaata gacattaagt gaactaattc aatcatactg
     8341 attctttcgt gtattcaaaa ttagatgctg tttaataaaa ttattctttt tgaaaaagaa
     8401 ttataactat atcccaaaaa ttgtaggtta atttaaagcc tatttcatca tcgtgattga
     8461 gcttcttttt catagacgaa tttctcagat agtttagttt tctttatgag atttttgtat
     8521 atttctgacc ttcgatacaa ctcattatga gtaccttgat caactagttt tccttgatcc
     8581 atcactacaa ttttatttac cttttcaata gacttcaagc gatgcgatat aattagtact
     8641 gtcttatctt gtattaaacg atttaaactt tcctgaatac gtttttcatt ttccgcatct
     8701 aaactggcag taatttcatc taaaattaaa attggcgcat ttttcaaaaa agcccgtgct
     8761 atggataggc gttgtcgctc tcctccagaa agtgaacttc cgttttctcc gaccaatgta
     8821 tgataaccgt caggtagttt ttcaataaag tcttcgcagt tagctaaacg agctgctttt
     8881 ttcacttcct catctgacgc ttgactattt ccaatacgta tattttccaa aatacttgta
     8941 ttaaacaaag ttacctcttg aaaaacaata gcgattttgc taaacaaaga agctggagat
     9001 acacgtttaa tatcataccc atcaattaaa atacagcctt catcataatc atacagtcta
     9061 gacactaatt tcaagatact agttttcccg gaaccactag cacctactaa cgcagtcact
     9121 tctccttgtt tagccgtaaa agagatatga tctaaaattg gggtattgtt atcatatgag
     9181 aaagagacat ctcgcaactc aatgtcaaaa gatttcagag gactatctga tccctcttgg
     9241 atactggttt ccttcatagc tctaattcgc tgtattttag gagctaaata aaatatttca
     9301 agcactgctt ctttcataga atcgaaggaa tcctttattt ttattgcggc tagtaagtaa
     9361 ccaacgacgt agagtatagt cacttcaccc gttaacagta agtgaacacc tactagaatg
     9421 actactgcta aactaacata tgagaaaata gaggataagg ccatgactga aaaagtactt
     9481 aattctacct ttaaatgaat cctttcactc tcttccattt ttttgaaaag tctgtcttga
     9541 acttttttag atagattaaa gctattaatc tcttgctgca attctatagt ttcttgaaat
     9601 tcttccgagt tttctctcaa agtatcgtaa tatttagtat tggctttcgt ttgggatttc
     9661 tttgataaca agattaagac aaaactaaat aacgttggca aaataacagc taatcccatt
     9721 ttgacattac caaccaaaag catcactgaa ataaaaggga aaaacagagc cataccgccg
     9781 gctttaggta ttgcatgact catcgcatgc tcaatacctt caacgtcaga catgatagtt
     9841 tgagataaat ctgataaatt atgttttgaa aaataggata atggtaaatt tgacaaatcg
     9901 tctgcaattt gcactcttaa atgcgcactt tctttataag tactattata taagttttca
     9961 tactccctat ctaacaacca atacatgaaa agcaaagtaa caaacgatac ggcaaaataa
    10021 agccaacggg gctttgtatt ttctaaaact aattcgtcta tcagcatcat tataatgaat
    10081 gctggtacca tattaatgca gtatacaaaa aaactagaaa tagaagcttt agttaaagct
    10141 tctgcgccct gatcacttaa agcaaatttg tttttataaa aacttttcat tgtcaagcct
    10201 ccaatcattt gcattctcat aattagtgac caattgttta tacacttgat gtttagccat
    10261 taattcatta tgatttccac gttctactat tttcccttct gacaagacaa gaatttcatc
    10321 tacagattta atgctggata atcgatgagc tatcataata actgtttttt ctttcatcag
    10381 atgttgaaaa gccttttgta gttcatactc attatctgga tcaatagatg cactggcttc
    10441 atccataata acaatctttg catcttttaa aattgctctg gcaatagcta tacgttgttt
    10501 ttctcctcct gacagataaa ctcctttact accaattaag gtatgctctc tttcgggaaa
    10561 tttttctaaa atcgattcgc aaccggctaa tcttagcgcg tcaaatactt tacttgctgg
    10621 cgcttgagga tttgctatag aaacgttatc gtaaatactc tgattgaata gtttcgtatc
    10681 ttgaaagaca aaagcaattg tgttaataat tgtgtctttt gaatagtgat ttaaaggctt
    10741 accaccaatt ttaatctgtc ccttatccac attgtagaaa cctgaaatta gtttagctat
    10801 cgtactcttt ccgcttcctg attttcctac taatgcataa attctttttt gttctaaaac
    10861 taagttgaaa ttttctaaaa cacactcttc tttgtatgaa aagctgacat tttcaaatgc
    10921 aatgtcacaa tttttaatct gtgtctcgtt cccatatttt agttggtctt gcttcatttc
    10981 taaataaagg gagtctagtg tttcgactgc atatcttgct tcaaacaagt gcatacttac
    11041 atacattatt ctcatgaaag aaacaaaaat aattccactt aaaaaaaaca acatcaacaa
    11101 gtcaacagct aatatcttgg gagtggtaag agaagttata aaaaaggtta taggaactat
    11161 caaaatgcaa actatgccaa aaaatagcca ttgaaaaaga acaaaaggtg ttttcccact
    11221 caaagaatac ttatatgcaa acttagcata atcgttaatt gctttattca aggctttaaa
    11281 tgatgttact ttagcaccaa atatctttat aacttgaatt ccgcggatat attctaccgt
    11341 ttcagagctt aacacatcta acgattcttg atatttttga ataaaatttg tattctctgt
    11401 cattttcttt aacaagaata caccaattac taacatggcg attagaacca atccaatttt
    11461 aaagttaatg aaaaagccta aggcaactga taaaacaggt gttactatgg cttgagcact
    11521 atctggaatc aagtgagcaa ttgccatatg tgttttagcc gcgttgtcat caattgtctt
    11581 tcgaatatat cccgaagaat ttaaatcaaa aaaacgaaaa cttgcttctg ttaaaccgct
    11641 aattcccttt tttctcaaat tagtctctaa gcgaaaccca aggatatgag aaaataagcc
    11701 agaaaaaata taaaataaag ctcctaaagt taaccaagca acagtttgaa ccgcataact
    11761 cttagctaaa tattcatctc ctacaataat tagagactga agaaatttgt aaatactgta
    11821 atatccatag actgttaata cgacagaaaa atcagaaaat aaagtggcta accatccgta
    11881 gtatttcatt ttagggacat aactaaataa ttttttgtat actttcattt tttcctcccc
    11941 cgcattcaat ttcttctctt attcgtttac aactgttaat gctttccgta atagcgtaaa
    12001 ttagagatgt acaaacagat aaaaggtaaa ctacgttcta ttttttgtat ttttttaaga
    12061 cttttcttcc aatcaataca ccaatcatag ataaacttgc tacaactaac actgaaatga
    12121 ttccccatat acctaccgca tagtggatca ttgcatccat tgttgcagca gattgtcctt
    12181 tatctatcca atcttgacga aaacgatctg taaagaacca tataggaatc cactgtccgg
    12241 cagcactgcc taattgcatt agagcccaag acgcaacttg aggaattgct cgatcatatc
    12301 caaatacaga tagaataata tcagctaaga tcgccatact aatcgaactg ataaaccaag
    12361 gtaagtacgc cccgcccatt aacgtaaaaa ataacaataa aacaattgtg taaatagtaa
    12421 aaattccttt tctagggtag ttatagctaa tataaacaaa tatgattcca gataataatc
    12481 caaaaatccc gggggatatg ctgtgaccaa acgccccacc aatactacta accattattg
    12541 aaaaaaagta aattactagg gaaataactg tagctaaacc aataaaaata taatcttttg
    12601 ttttcatata tattctcctc aattctattt cgttatgtca ttaaaaatct gttctatcaa
    12661 agttgaatct tctactctaa gttttatatc tttttttatt ccaccatttt ttaaataaat
    12721 aattctattg catgttttat aaagaaattc cggatcatga gtagctataa ctactgcacc
    12781 cttttctttt aagtctttaa taaggtcagc aatcctttcc atattaatat aatccaaccc
    12841 acttgtaggt tcatcaaata gaaataaatt agtttttgac aatgaagcta tagaaaccaa
    12901 taatctttgt ttttctccac cggataaatc aaacggtaat tgatctttca ttttataaag
    12961 ttccattttt tttaaaattt tttcttgctg atctttacta actatttcat tccccagggc
    13021 taactcgctc ctcactgatt ctgtgaaaaa ttggtaatcc atttcttgca ttactaaaaa
    13081 cggcgatgac aaattttttt tattttcgat tatctttcct ttggtagggc gaataatctt
    13141 catgagtatt cttagtaagg ttgttttccc cacaccgttt ttccctacca aaccaataac
    13201 atctccaact ttgacatcaa aatttatgtc ttttagaata ttttcataag taatttcttc
    13261 aacttttaat acatctgcac ttaccttttc gatataggga acttcaatcg aactattaaa
    13321 aatgtcaaat ttccttaatg cgttattgtt ttcagttttt aacaactgaa tagttgtagt
    13381 ttttacagtt ctatttttaa caaatagaac ttgatcaatt aattctttca aatagtaaaa
    13441 acgatgttca gctataacaa tagtaaatcc tttatttttt aaatcacaaa gtaagtcagc
    13501 aagtttctta gtcgctttga agtccaaatt tgaagacggt tcatccaata taattaattt
    13561 agagtttagc gataaactac atccaataga aattttctgc cgttcaccac ttgatagcga
    13621 aaaaatgttt ttatctaata tatttgaaag atctaataat tcagctaatt cattgattct
    13681 tttatccata tgctcttttg taaatccaaa gttttccatc gaaaatacta actcactcgt
    13741 tgtatttgtt gtaaagaatt gtcctctagg atcttgactt acgtagccaa tattttttga
    13801 tatactttct tgtgttaagg attgtagcga gttattttca aataaaattt ccccaaccaa
    13861 ttttccgtca taaagtcttg gaataagtcc attcattaac tttaatagtg tggatttccc
    13921 actcccagaa gaaccagtaa tcaatgtaag actgcctttt tttattgaaa aattaaggtc
    13981 aacaagcgct tgttccttaa catccttata tgaaaaagaa acattgttaa attcaaacaa
    14041 ttctttcacc tcaaatcaaa atacttaaga aaaaaatagt aacggaaaac aggatgatat
    14101 agtctaataa gctcatgttt gttttatagt aagaacttct tttcacagga gattctatcc
    14161 cctttgttat tgcattagcg gtcatttctt cagcaatcat agttgcacgt ataagaacgg
    14221 gaactaacag aaattccaaa tattgcagag gctgaaaaaa agacaagcga atgtttctca
    14281 tttttaaaga ttctttaatc attcttattt cagaacgata agtcggaaag aatctgatca
    14341 ctactgtaat acttaacatt atttttttcg gtaaatgtat tttttctaaa gaggataata
    14401 attcattgct agtatagaca ctaaccaaca accatgagat cattacgacc ggcaacattc
    14461 gaaatacaat atataaggat gtcgcaaaaa atatagtaaa aaactctatc ttgatagcat
    14521 caataatttt cattactaaa aacagaactg aataaatcaa gatgaaataa atacatgtct
    14581 ttattttacg aaacatcaat actaaaactg tagcaataat gaaacacccc acttgaaata
    14641 tatttccctt tcctaaaaaa ataatgaccg gaaaaaaaat atttaaagta atcaaggtta
    14701 acggattgat ttttgacact ctttcacctc caaaaaataa gatcgcactg ttatcttaag
    14761 ctgataaaaa tgcaccgtta ttcagataac agtgcgatct tatcaaaaag cataactgag
    14821 aacaattctc aatgcaagct ttttttaatt aactcagtaa ttcaatccac ccaggaacag
    14881 aaaattgata caatcttctg gttaatagca tagtcttttc ttttgaatag ctatgcttca
    14941 atatatcata gaatgtagcg tagaatgagt aaatgtagaa gtggatttct tcttcattaa
    15001 agactaaatt ttgattgatc attccgatag tctttaactt atctagacaa cagactgtct
    15061 ttttcgtcac aaatgatgta attttttcaa caatatcttg atatttggtt ccagctgatt
    15121 ggaataaaat caaatcgaat atacgtttat tctcaaaaat ataattaata aatggatcta
    15181 attcattgaa gttctctctc caacttttct ctatatctat attttctaag tttaagaggt
    15241 atttggtaaa ctcttcatta tacatattaa taagcgcatt aatatgcggc tgaataattt
    15301 cttcaaataa cgcttcctta gaagtaaaat gtttataaaa agcacccgta gttacgcctg
    15361 cggtcaggca cagttgcctt atcttagttt gctgaaatcc ttctttttca aaatggctta
    15421 gtccactttc tattaacttt tggtgagtta taatataatc tctggtttct atgggtactt
    15481 cctcctaata agatgaatta actttattat cttacacagg acttgaagtt tcaatcgtta
    15541 acgtttatta ctgaactcat tccttagagc taaactaatg agaaagagca gaataactga
    15601 tacaaaaagc aatcctacaa gaattaatag actcatctaa atcattcacc ttttctaaaa
    15661 taatccgaga aagatttaaa aaaattatat ccagctatta ggataagtcc tatcataatc
    15721 actccaatag ccattttctt atcctcccta aaaataaagt cttttactta aaaccttttt
    15781 tgtaatcgtc aataaatttt ttccctttat cagttaattg aaatcctata tctaaatagt
    15841 cctcatatct aagtttagcc agcatctcac ttacagatgc tggactaaca cttagaactt
    15901 ttgctaaatc attatttttt aatttctttt ttctttttga taattcctga attgctagta
    15961 aataaattag tatagtagtc gttttaattt gtaccatacc taccctccaa attatttcga
    16021 aaggccttca gcaattttat ccaggttcca tttcatcatc gcatagtaac tatcaccatc
    16081 ttgtcctttt tctgcaattg aatcagtaaa aatcgttgaa tagataggaa tattggtatc
    16141 ttttgatact gttttcattg gtctatcatc cacactgctt tctacgaata aggagggaac
    16201 ttttgttgtg cgtaattttt caactaagtg ttttatttga tctggtgttc cttcttcttc
    16261 agtgttgatt tcccaaatgt aagcagaagg cacattatac gctttcgaga aatatttaaa
    16321 gcatccttca cttgtaacaa tcattttttt atcattcgga attgaagcaa atttagattt
    16381 agcttctttg tctagagaat ccaatttttc aatatactta tctagatttt ctttatagaa
    16441 ttttttatta tcaggatctt tttccgctaa ctgtttttca atatttttag cgtaaataat
    16501 accgttttct aaatttaacc aagcatgagg atcttccttc cctttctcac tctgaccctc
    16561 taagtaaata acatctatgc catcacttgc tgcaaaatag tctttgtttt cctctttgtt
    16621 cgcattttta actaattttg taaaccaagc atttcctcca gtttccaagt taacaccgtt
    16681 ataaaaaatc aaatctgctt ttgaagtttt ttgaacatct tcaggcaaag gttcatattc
    16741 gtggggatct ttcccaatag gtacgatact gtgtaaatca attttatctt ttgctatatt
    16801 ttcggtaata tctgctaaaa tcgaattagt aactactact tttaattttt cgttcgaagc
    16861 cactttatct ttgtctttac tatttattga acatgccccg aatataaaaa gagcaatcaa
    16921 aatccccaga cttcccagta ctgctttcca ttttctcatc ataacgatct ctccttcttt
    16981 cttaaaaatt tttgttttgg tgagattaga aaactaatta caaataagaa tgccgctgtt
    17041 aaaacaatac ttgatcctgc tgctatgtta aaactataac caataaacaa tccaagcaat
    17101 gatgaacttg ctccgactgt tgcagaaata aatatcatag ttttcaagct atttgcatat
    17161 aaatatgctg tagctgctgg tgtaattaac attgcaacaa ttaatatagt tcccacactt
    17221 tgcatagctg ttactgctac taaagttaat agaaccatca atagataatg ataaaaactg
    17281 acattcatcc ctattgcttt tgccattaat ggatcaaaag aagtgattaa taattcttta
    17341 aaaaatagaa ctactataac caacactaaa accgaaacgc caattgtaat ccacttatca
    17401 atatcttgta cagctaaaat atttccaaat aaaatatgaa ataaatccgt tgagctattt
    17461 gcaactccta taaggataac tcctagtgct aaaaaagaac tgaaagtaat accaattgct
    17521 gtatctcctt ttattacact attgcttttt ataaaagtaa taatgataga agctaataga
    17581 ccaaatacaa tcgcaccaat aaaaaaatta atacctaaaa tataagaaat tgctactcct
    17641 ggcaaaacag catgagaaat agcatcaccc ataagagaca ttcctcttaa aataataaaa
    17701 caacctactg ttccagaaac aatccctata actacagatg taattagagc attctgtaaa
    17761 aagtgaaagt catacagacc attaataaaa ttttgaagca tattattccc cctctccaat
    17821 aaatatagta tctccatagg ctttttttaa attttcttca ttaaatacag attctacact
    17881 tccgtgagca attagatttc gattcaaaag aataatatta tcaaaatact tctttacctt
    17941 gcttaaatca tgatgcacaa ttagcactgt ttttccctga tgtttcaatt tttttaacgt
    18001 atccataatt attcgttcac taactaagtc tatccctaca aatggttcat ctaaaaatat
    18061 gaaatccgca ttttgagcaa gacatcttgc taaaagtaca cgttgaaatt gtcctccaga
    18121 taattctcct atttgatgat tagaatattt tcccatatcg accttttcca aagcctttga
    18181 aactctttgc cattctgcat ttttaattct ttgaaaaact ttcattccgg catatgttcc
    18241 catagataca cattctttta cttttattgg aaaagtaaaa tcaatatcag ctttttgctc
    18301 tacatatgca atcttcacca gttttttatt gatatcttca ttattaatgg agacctctcc
    18361 ctgatgaggg ataatattta acattgcttt aagcaatgtg gattttccag caccatttgg
    18421 accaataata cctgttaaag caccttcttg tatttttaaa gaaatctcgt tcaatgctaa
    18481 aaaatcgtta taacaaacat taagcttttt cacttctaac ataactttct ccttctaaat
    18541 gataatgatt atcacgatca tatggtatac aaaaattata ttttagtcaa ccctaaattt
    18601 tatagtttag atttttttct gttgctgaag attagaatga caaaactatt tgaaactacg
    18661 cgtatagtaa gtagctttca aaatatataa aaaaggagcg ttctcaaatg agaacatgct
    18721 cctaaaacta ctttattggc gtatggaaaa attatacttg aagtggatag cgggttgaat
    18781 aagttgataa gcatcaaaac atttataaaa attattcaag actatgtctg ttccatatac
    18841 aaatatgaaa atcagtaaac tccatttaac ttttttcgta gtgtgtcttt ttatacatgt
    18901 ttatcgttat taatagtgaa taggaaaaaa actatttttg atcattgaca attaaataca
    18961 atttttgaat aacgatccct gctattcgag ctagaaactc aagtacgcca tgacctgatt
    19021 agcgacaaat acttagagat ttctttttgt tctgggaaaa caaaatgcca tgacaatagc
    19081 agcaaataat gatacttccg taaggctcgc cgtaaactgg ggagaggtga aattcctatg
    19141 tgcttaccca aagcaattca aaaatttcat aactactatt ttaggagatg attttataga
    19201 atccagagtc tacctgaata aaaaacaaac ctgtgcattt ctaggaatcg ataataatac
    19261 tctagataag tggattcgta tatataattt cccagtaata agagttggtg gtgtagttcg
    19321 ctttgacgta gaagatatta aagactttat gaatacttga aattttcttc tcgccgcata
    19381 tactaaaatt agtgaaatat actgccttac tttttttaag gagtgaggat tttgcctaca
    19441 aagctttcaa atgggaaata taaaactaat ctgagatatc caaaaaggtt taaagaaata
    19501 acaggaattg catcagaaaa gtatcaaaaa acatttccaa ataggcaatt ggcgattaaa
    19561 gccgaaaatg atatgaagaa gaaaatagaa aaagttttga gagaggagaa tgcaaactca
    19621 ttagaattaa aaggaaaaat aacgtttaaa aaattttacg aatctaaatg gcttcctaga
    19681 tatgaattag gtcaaacaat tagatcaaac aggcctccta gtgatataac cattagtaat
    19741 acaaaagata tatttcggtt gcatatttta cctatgtttg gtgaatacgc aatgaattat
    19801 ctaaatatca atactgagat aatatcagat gaattaacca aaaaatcgaa agaatatgca
    19861 aatataaaaa tcataaaagg atatgttcgt tccatgttcg atattgcaga aatactaaat
    19921 tatatcgaat ttaatcgaac tactaaaatt attcaaagta tcactgcacc taaaaaaaat
    19981 gctttagaag aaaaaagaat acaagaaggt aagcaagcac tatcatcaaa agaacttact
    20041 aattggatag aagcagtaaa tgatgatttt aataatcatt tacttacttt tcatgattac
    20101 acacttttta tgattaccct gtatcttggt gatagaaaaa gtgagactta cgctttgcaa
    20161 tggaaatata ttgattttga aaaacaaacg gtccgtctta aacatacttt agataaatat
    20221 caaagaaaaa agtttacaaa aggtcgaaaa gataccgtca ttcaagtacc agaagttgta
    20281 atgacattgc taagtgaatg gaaatcggta caagctgatc aattgttgaa attaaaaatt
    20341 aagcaaacgt tagatcagta tctctttact tatactaaac catctggaga agtaaattgt
    20401 cctgttcatg ccgattacct aaactatcga atcaattcaa ttaaaagaag gcatcctgac
    20461 ttagttcatt tatctccaca caaactccgt catacttatg caactatagc tagacaaggt
    20521 ggagcagata tgaatcaaat ttcaaatgct ttaacacact cagatatttc tacaacaaag
    20581 atatatgtga acacaccaga tatagttgat aaggctgttt ttgaagcttt tcagagagga
    20641 ttgaataagt gtgattgata tgtgttctta aatgtgttct taaaaacaaa agaagcactt
    20701 tccaaataat tgaaaagtgc cagaaacgtt gatatataaa ggatattaac gttttgagaa
    20761 ttgtgaagct ttacgtgctt tcttaagacc tggtttttta cgttcgacca tacgtgcgtc
    20821 acgagtaagt aagccagcac gtttaagagc taaacggaag tcaggatcaa cttctagcaa
    20881 tgcacgagcg ataccatgac ggatagctcc tgattgtcca gcgtatcctc cgccgtttac
    20941 gttaacaaat acgtcatatg cgccttttgt ttcagtaaca gcgaaaggtt gattgattac
    21001 ttcacgcaag tcagcatgtg gaatgtattc ttcaacatct tttttattta cagtaatttt
    21061 gccagttcct ggtactagac gtacgcgagc aactgcgttt ttacgacggc ctgtgccgat
    21121 atattgaact tgtgccaatg aaattccctc ctattagatt aagtttgtga tgtctaaaac
    21181 ttctggttgt tgtgctgcat gtggatgttc tgctccaccg tatacgttca attttgtaaa
    21241 ttgtttgcgg cctaaagtgt ttttaggaag catacctttt acagaagttt cgatcaaacg
    21301 acgagaattt ttagcacgca attcgccagc agtgattgat ttcaatcctc ctgggtagtt
    21361 gctgtgacgg taataaattt tgtcagtcgc ttttttacct gttaatttca ctttgtctgc
    21421 attgatgacg atgacgaaat cgccagtatc caaatgaggt gtgaaagttg gtttattttt
    21481 accacgtagt acagatgcaa caactgttga aagacgtccc aatggaacat cagttgcgtc
    21541 tactacatac catttacgtt ctacttcgcc tggtttggcc atatatgttg tacgcacgtt
    21601 attttcctcc aatttaagtt ctgatgtttg acatacactt cgagtttccg gggctctttg
    21661 tggggcaaac aataccattt aacatattac ccgctaatct agtcaatgtc aacgtttttc
    21721 tgcttgattg aaaaatttat atgaaacttt acttcttttc tttcttttat ataaaagggt
    21781 cgtttcctgt caaaatgaaa aacaaaaaga ggctgtgaca aaagtcttgt cacaacccct
    21841 tattttgaat aaacggtgtt ctcctgccaa ctcctcggtc acaagtcaca atatccataa
    21901 aacataagaa gctgtttttt aaaattgttt cttgtgactc ggagctgaac ggcaggctta
    21961 caacctcttg gtaaacggtg ttcaatcagc tgaccctcgg tattagctca aaaaacggca
    22021 aaatatgagg aactattttg acgtttgttt ctctaatact caggtcaaaa cgctgatttc
    22081 acaacctctt tttcggtcaa gttcaacaac agtttttacg cgtcgatctc tggcgctttg
    22141 cccaaatgag cttggatcat ccagatacgt ttttctgtgg ctgttttgaa ttcgataaag
    22201 atatcttgtg ttggatcgtc gccttcttct ccagaaactt cgattccttt ttggtaaaga
    22261 tctgctagat aacgatagcc tgcaacaagt gtttccaaac gttctggaat agtacgatcc
    22321 cagcggccaa tttcatcagg gatttttgtg ttatctgcaa attcacgcaa tgtagaataa
    22381 ggtgaaccgt ctaatgtgat caagcgttcg gatacttcat ctaactgagc gttcaaatca
    22441 tccatgaact catccatcat tgggtgcaat gtaaggaaac ctggtcctct catgtaccaa
    22501 tgtgtttggt gaacaacaac agacatttgg cttagatctg caactaattg atttaatact
    22561 tctttggttt gttggaattt catctttctt gcctcctaac attttcttac acttaaaata
    22621 atagtagact ttcaatgaaa agtacaacaa aatcatttac ctgagaacga ttttcagttc
    22681 gcttttttat ccgtaaagcc aatcattttt cagaatcagc taattcttct cgcagttgag
    22741 caaattgctg ataagtctgt gctaaatgct tttgtgtcga aacaaataat ggatagattt
    22801 tctgatattt tttatgatca gtttcttgta ctccaacttg ctctttcaat tctatcaatt
    22861 cctgtgcttc actgatactt tggatctttc cgatgcttct ccagcctaaa atcacagctc
    22921 ctaatgcgga tgcttctgtg acagtaggga aaacaagcgg gtaacccagt acatcagcca
    22981 tcatctgacg aaaaatcgga gattgagcaa acccgcctgt agcacggatc tctgtgagct
    23041 gattttcctg gcaaactcct tttaaaatcc gataaaggtt gagacagatc ccttctagaa
    23101 tcgccttcat catttctttt tctgtatgtt gccgctgtaa accgataaaa ctgccaaacg
    23161 catctgcgtc ccaaagcggt gctcgctcac caagtaaata gggatgaaaa atcagaccat
    23221 tggcacccgc tggcacaaaa gcgatagccg aaagcagtgc atcatatgga ttttctcctt
    23281 tttctttagc agattgaacg acttcctgta aaaatgtatg ccctgcccaa tcaaagatgc
    23341 cagcaccatt gctagtcgct cctcccacta cccaatgttt ctgatccaga acataacaaa
    23401 acgtctccat ttctggatgg aactgaggct gagtagtaat aaaacgaagt gctccgctcg
    23461 ttcctacggt caaagcggcg atcgtatcac caaaagcacc taaccctaga ttagaaagcg
    23521 gcccgtctgc ccctcccaaa acaaatgata tatcatttgg caaacctagt tctgctgctg
    23581 tttttgatgg tagacctgtt acttgatagg tggaaggaaa gacttgcggc aattgctcta
    23641 ttcgaatgtt caagtagtca agcacttctt ctgcccattg gaattcatgg atatcaaaat
    23701 atccagttcc tgaagctgtt ccgtagtcgc tgatcagttc gccaaacaat ctataaaaga
    23761 tatagtcttt gataccgaca aaataagcag tttttttgaa taattcaggt tggctgtttt
    23821 gcagccactt gattttgttc aatggtgtca ttggatgaat cggcgtccca gtctgccgat
    23881 aaaattcttt gccaagcacg gtatatttca gtttttctgc ttcgtccgct gctcggttat
    23941 ctgcccaagt gatcattcga gataacggtg ttttctcttc atccaacaat atcaggccat
    24001 gcatcgcaga ggagaaggag acaaattgaa tgattgtgtc tgaaggcaac tgtttaatta
    24061 cttcttttat aacggtgcag acaccttgaa agatcaattc ctgatcttgt tctgccatcc
    24121 cttgtgtttc ttggatcaaa gggtagcgaa aataagcgga tgctgcttct tgggcttcat
    24181 ctgtgaaagc aactgctttg gtttgtgtgg ttccgatatc taatccgatt gaaacgttca
    24241 ttttttcacc tcaaatttta tgtatgtaca aaaatttagt ttaagcttgc gtatcttggt
    24301 tttgaaaagg gggagatggc gtgttacttt attttatcat tggttgctgt atcggatcat
    24361 ttttatgttt aaccgcccag cggattccgt taggtcactc tattatttat cctagatctc
    24421 attgtgtcaa atgctgtcgt tcgctttctt ggtatgaatt gattccgatt ctctcgatca
    24481 tcgtccaacg atttcgctgc cgctattgca ggtgtcggtt gcccttctac tatttgttgg
    24541 cagaagcctt atgtggtggt ctttttgcct ggttctttac tttttcttcc gcaagaaatt
    24601 tttcgacgtt catatggatg ctttcggctt tgctattttc gttgatggat ttattttact
    24661 tcatggtcca ttctcacacg ctattctgtt cttgggcaat tttatggatt ttttggttgc
    24721 aggccggcgt cttccaatgg caaagtgtgc tgttaacgtt tatcgtaggc ggtgtgtgcc
    24781 ttcgctatgg tcaatcctat ctcggagctg gcgatactct gttgatgctt tcttggagtg
    24841 gtggcttgtc tctagaagag ttgcttcaga tcttgtttct tgctagtctg tttggaatat
    24901 ccgttttttc tgtttacttt atctgtcata aaaaaaagtt ggaaaagctg cctttcattc
    24961 cttttttgag cagtgcgttg ctgattgtcc ttcatcttag gtattgaaaa aaggttgtga
    25021 caaacgactt gtcacaacct ctgatatata gtgttctctt accaaatcct tgtgacttgg
    25081 agctaggcga caggttcaca acctctccac taaatggtgt tcaatcagct gaccctcagt
    25141 attagctcaa aatgctggtt ttacaacctc tgatatacgg tgttccataa gtagcttttt
    25201 cggcttctta tttcttgacg ccccaatgtc gacgaaggaa acctgcacgt ccagaacctt
    25261 ctcgataaag attgtaatgg agcttgtttt tcttgtaata atcctgatgg taatcttctg
    25321 caggataaaa ttcgcttctt ggttcgattt ttgtcacgat tggcttatcg aatcgcccgc
    25381 tgtcttgcaa tgcttgtttt gaacgctctg caatatttcg ttcttcttca tcattatagt
    25441 agatcactgg acgataagaa tcaccgcgat cagcgaactg tcctaatgcg tcagtcggat
    25501 cggtttgctg ccaatagatt tccaccaact gatcataaga aataacttct ggatcatatt
    25561 cgatctctac tgcttctgta tgccctgttg tgcctgttgt tacttgttca taagttggat
    25621 ttggtacatg cccgccagta taaccggatg tcacagaaat gatccctggc tgctcttcaa
    25681 atggtttgac catacaccaa aagcatccgc ctgcgaaaac tgcttttttt gtcatataaa
    25741 atcccccttt tttctcatca taccttgatt tccaaaagga ggataacgtt ttgcttgtga
    25801 cagaaaaaac ggaaactatt atatacgtga tacggcttct gataaatgcc aaaacgaacg
    25861 tacttgatca aaaacggtta aatcaggaat cacatattca gcagaaggaa gatttatgtt
    25921 ttcacctatt cccaatacat gcatgttacc agccaaagct gcttgacaac cagcttctga
    25981 atcttcaatg accaagcatg catttggcgg tacattcaat tgctgtgctc ctttgagaaa
    26041 aacttctgga tcaggttttg cttttgatac ttgcgtgccg tcaataagga catcaaaata
    26101 acttgtcaaa ttcgtttttt ctaaaatgag gcgtgcattt ttgcttgctg atcccaatcc
    26161 gatcttgatg tttgtttttt tcaagtactc aagaacagct attgcaccag gcaaaacgga
    26221 tgtttcatcc attttttgaa tcgcttgaag atatagattg tttttttgct cagcaaaggc
    26281 tgatttctct ttttctgaga atttctgttc catttttcca aatgacagca atcgctctag
    26341 tgactcggtt ctggatattc ctttcagttg ctcgttttct ttctcagtga atggaatgga
    26401 cagctggtta gctaaaacca gccacgcttc gtaatgataa cgagctgtgt ctacaagaac
    26461 gccatctaaa tcgaatagtg ctgctttgat tttcatcgat tacctccttg aaaatgaacg
    26521 acttgaagta aagatgcgat gtttttagct gtgcgatgaa gattatcagt ggcttgctgt
    26581 aaagcttctt gtaaaggcac agctgcagga aggatcggaa aaacggccga aatgccaaca
    26641 tctgctgctt tttcgcttcc tttaccaaca cttccgcaaa tagctatcac aggaacgccc
    26701 tctggtactc ttctagcaat cccaactggc gcttttcctt ttagactttg tccatctaga
    26761 cgtccttcgc ctacgattac gaggtctgct tgctgtaccc gtttatcgaa ctccagtaaa
    26821 tccaatacgt agtcgatgcc agagactatt ttccctgaag caaatgctaa cagtcctgca
    26881 cccattccac caccagcact actgcccgat gcctcgatta cttgaggggc aaatctcata
    26941 taaaaatcat gcatggcttg gtcagccact gctaattcat tttctttcaa gcctttttgt
    27001 ggtccgaaaa caaaagaagc gccttctttt ccgcataaaa gattagtaac atcagtaatg
    27061 acagtgattt tggctttaat cagctcaata ggcacgttcg ttttatcaac tgtagcaatt
    27121 ttacctagat tttctcccag tgcttctact ttttgattat cttgatcgta aaaagcataa
    27181 cctagacctt ctgccatgcc gatgccgcca tcgttggtac tacttccgcc aacgccgatg
    27241 atgatgtgtc gaacaccttg ttgcaacaat tgtcgtatca gttctcctac tcctcgcgtg
    27301 gataacctaa gtggctgttg ttggtttagt gggacatctt tcaatccgac gatttctgcc
    27361 atttcaaaaa tcgcagtttg accgtctgaa gcgtactgga tttctttgtt tttgccaaat
    27421 gcattagtaa cgactgctgt ttgccgcttc aaccttaaac tgtctgctaa tgatgaaacc
    27481 gttccttctc ctccatcgcc tataggtagc agatcatatt cagcctgaac aaaaacttct
    27541 tgaaaacctg attgaatggc ttgtgctgcc tctaaggctg tcagactttc tttgaaagaa
    27601 tctggcgcaa tcagaatacg cattattttc ccctccgttc caagatcagt tctttttcca
    27661 gctggtattg gtctccattg acaaaaatag gtaatgtttc tttcgtctct ttttttatta
    27721 ctatttgttt ttttgtcaaa acaaatagca acctgtgatt tttccaaata aaaggaaaag
    27781 aaattttttc ccatgctgaa ggaagtcttg gagaaataga aagagttcca ttcgttactg
    27841 taatacctga aaaaccaaat ataactgcta gccatagtgc tcctaatgaa gcagcatgaa
    27901 ttccgtcatc tgatgaatgc ggatctggtc ctaaatcaat caggcacgct tcttggaata
    27961 aatgatacgc ccattccatt tcttggatac ggcaagcctc tatggcgtga atcgctttac
    28021 tcaacgatga gtcgtggatt gttttgtttt catagtaatg caaattctta gaaacgattg
    28081 gttgagaaaa atactcaggt aataagtata acagcatgac aagatctgct tgcttgctga
    28141 cttggagttc atttacttcg ctacgggcat aatcaagtaa aatagactgg ctgccaggac
    28201 gttctttgta agtggtcaaa tcaataactg gcttactgag atacgtgtca tcttgcggaa
    28261 tcagctgttc tttgttaggc actggcaaat ataacttatc taaaaaattt tgacattctt
    28321 tcaaaaaaag cggatcgcca gcttgatatt taacagctaa ggccacatta taatgcgcca
    28381 gatagttcgt atatgcatta ttgtcgatat attctgtgta ttcatctgga ccaatgactc
    28441 tgtgtatctc taaatgatca tcgactttta cagctctgct cagccaaaaa gctgctgttt
    28501 catgaatgag cgtttttccc tttgtttcta aaaacgattg atcctctgtt gcttggacat
    28561 actcttgaac ggcataagca aggtctgcac tgatatgatg ttctgcggca gctgaagcaa
    28621 cttcttgtct tgttccggtt ttgatattca atgcagcaaa tttgggtgtc tcttcctgac
    28681 cagtgtgtgc gctttcccat gggaaaagtg caccttgata gtgataatct cttgcttttt
    28741 gccgtgcgcc ttctaatcga tcataacggt acagcagcaa attctttgct acttcaggaa
    28801 actgatataa aaagaaaggc aggataaaaa tttccgtatc ccagaaaaca tgccctttat
    28861 aaccttcgcc agttagtccc ttcgccccta cactaacact tggatcattg cggggagtca
    28921 taatctgtaa atggtaagca gcgaagtcca tcgctaactg atcgaaagaa gaatcgcctt
    28981 ggatcgttat ccgatgctga tgccaaaaat ctgtccaata agacatcgac tgctctaaaa
    29041 tttctgtata actaacgctt ttctgggctt gggcaatact tttcttaaac gggtctgttt
    29101 gctgaagaga ggtagagagc acgatgatct tctccagcgt aaaggacgta ttttttctga
    29161 tcttttctga aaaggaagcg gtgatccggc gattttttgc ttggaataca cctgctttat
    29221 tgaatttagc tgcaatccca atatcaattt tactttctgt tgtctggtag gatgcactga
    29281 tcaaatcgtc accaaatact cgcagttctt tttcaaccaa ctgctgtgta ccaaaattgg
    29341 tttgctgtcc atcgataccc gttttcacat ctacttggca gtcttcggtc aacggcttga
    29401 tgaccacttt cagggctgcc tgatggagat tattttgatc agcaacacga caagtaatga
    29461 cttccacttt ttttccttga gcagtttcaa tgagcgcata gcgtttaagc tctcccattt
    29521 ctaaatctag tgcccgctcg tatgcaagaa ctttcatcgt ctcaagagaa aaatgttcac
    29581 cgtcgatttt tatttcccat ctgatgacat ctgggagatt cactaactcg gctgattctg
    29641 ctcctaaagg tcggttatat acaccagctg caaacattcc ccttacttga ttccgatagt
    29701 cttcctcctg gcttcctctg ataccaaaaa atccatttcc aatggtcatc tgactcgcat
    29761 acttattgat atactctaaa tcaaactgtt tttcttctac tttcataagg taataacttc
    29821 tcctgtttct gccgaacgat ataaattttc caccagattc tggatgacat atccttgcca
    29881 gccatctgca atcatctgag tctggtcacc gagcgcccga ttcacaaaat gagccatact
    29941 tagttcatgt gggtcattaa ctggcgtggt cttttgtaag gaaaccagct ttccatctac
    30001 atcttcgaat agttcgcctg gatacagatt agcacctgcc tgatcgccac aaaaagccac
    30061 attcagtttg tcttctctga tattcaaagc aaacgaagta ttcagttgga tgatcttgcc
    30121 gttttctaat tccagtgtgc caaaaattgc atcttctact gtgaatttgc ttggatccca
    30181 tttcccgaat ttccctgaat tttttttcgt accaattttt tggaagctgt acgcccgaac
    30241 gctttttact ttaggaaatc cagtaatata catcgctgta tcaagcatgt gaatgccata
    30301 atcgatcaaa ggcccgcctc cttgaagttc tttgtcgata aacgttcccc agcctggaac
    30361 accagaccga cgaagcgcat tcgcttctac gtaataaata tccccgagaa catcaagatg
    30421 attcttgatc attgttgctt cgtcagaaaa acgatgctgg aatccataag ccaaaatctg
    30481 cccgttttta tcggctgtct ctgccatctt tcttgcttga tcagctgtca tagctggtgg
    30541 tttctcgcac atcactgaag cattagcttc gagggcagcc aatacatgtt cgaagtgaaa
    30601 gcgattgggt gtacagacac tcacgatatc gggagaaatc tcattcagca tttgtgtaac
    30661 tgatgtatac gcatgagaaa atcctgtacg tcggcaaaag tcttctgctc gctggctgac
    30721 tggatcaacc acagcggcca attctaagac atctgatctt ttttgataat aagcagcatg
    30781 tactttttct gctacttgtc cagcgcctat gattgctact ttcttcattt atttctccct
    30841 cctttgctta tttacttatt gttattcttt ctctattctt tctctaaatc atttaaatga
    30901 cgcgctgttt ccagacagtt tttcaagtat tgcaatgatt cctgataaat tttcacttca
    30961 ttttctcctc gcaaacgaca ttcatatacc atataacctt catactcatt ttgaagtagc
    31021 gtttgaaaat ggcgagtaaa atcaattgcg ccagatcctg gttggtagcg atgattatct
    31081 gctaaatgga tatgacccac atactctttt tgcgtagtca atgcttttga aatatcatct
    31141 tcttcaatat tcatatgata aaaatcagcg atgatctgca catgtttcaa ttgattagag
    31201 tcaatgtaat gacgcgcatc cgctaacaaa ttgatcatat ggtcttggta tcggttcaaa
    31261 ggctccaaat aaataaccgt acctgtttct tcggctactt tatctaaata acgaagtgat
    31321 tcgctgactg ctttatagtc tccttctttg cttcggggtg aaaccatcgg cggtaatcga
    31381 taagtgaaca ttccccaagc tgctgggata acgattcctt tgcctccaac ctcgcttagt
    31441 gcacgcaaaa tctcagcaat ttgacgtaca ccattaagac gccgttcttc gataaagtca
    31501 ccgatccacc catcgtagcc gttacaagct gtcactactg ggatccctgt ttctttgatt
    31561 gcctgtttga ctgtgtcgat attttctatt aacaacttac cgtcgatttc gtattcttca
    31621 aatccacagt ctttgataaa tctaaatttt tctaaaatgt ctcttgggaa aaatgcttga
    31681 tcttgcgtac cgaatttcat tgttctcctc ctcggacttg aacgcccatt ttgatacttt
    31741 cttctgggtg ctggtctaca taacgcatgt aagcttctgc gctttctgca aatggcacaa
    31801 ctggatcaat gatttcttca caatccaagt aaccgttcat caataattcc caacatgtgt
    31861 cctcgattcg tttacggttc catcttgggt aatctgggtt tggttcacta gatgctcgag
    31921 caaagacgat ttttgcatta ttgaaatgtg cttcgcgacc aagattaaac ccagctggaa
    31981 aaggtttagc aaaggcaaca taagaaatga tcccgccata gccgatccct cttaaagaag
    32041 cttgtaatgc ggcagcactg ccactcgttt caataactga atctactcca cgtttatctg
    32101 tcaattcctt tagacgaaaa ccgacatctg ctttcatagg atctagcgtg taatcggcac
    32161 cgtatttttc tgctaattct ctacgctgcg ctatgggatc gatcccaacg atggtagtag
    32221 cacctgcttt tttagctaat tgaatcgcaa tttgcccgat agcgcctaaa ccgaagattg
    32281 ctacagcatc acctgctcga acatgtgctt cccggatacc acctaaagcg aattgagcag
    32341 gatcgtaaca tactgcattt ttccattgat ccaaccgatc aagtttacgt agtttataat
    32401 tatcaacacc gtttgtaatc agcgtttcac gaatgggtcc ataagtgcag actaactctc
    32461 ctttttgata ctctgtcacc aaactgcccg tttcttcgat cgtaccgaca atcatatttc
    32521 ctaattgaaa ttcgccaaat accacaccgc gttggcttcc ttcttctctt ggcaagaata
    32581 ggcgccattc ttctgaaaat tcttcatcga taaatggact taatcctcta aagtcgacaa
    32641 tttctgttcc tggttttgga gtagcgaaaa ttactcggat acgtacttcg ttcttttcga
    32701 tctcacggtc ttcgtaatct ttgaatccgg ctattcttgg ttctaatgca actaattttt
    32761 tcatcttttc tttcctcttt cttttctttt tatcctttta cgccgccttc gactaaatca
    32821 cctttaataa atcgttcaga taacgcatac ataatgatga ctggtaaagc ggtcaccaat
    32881 gaagcggcca tcattcgtcc ccaaatgtag tctggtgtac tgaataatgt gttcaaaccg
    32941 attggtaaag taaatttttc ggaggtcgac aggaaaatcg aagcaaataa ataatcattc
    33001 cacgaaacca taaagcaata aacgaagaca gaaatgattc cagaaatagc caatggtata
    33061 atgatccgaa taatgatctg gatccggcta agaccatcga tcattgctgc ttcttcaata
    33121 tcagtcggaa tcgtgtcaaa ataacttttt agcatgaata tcgcagtagg aagtgtttga
    33181 acgaccatcg tgataatcag tgctgtattg gtatcataca aacctagacc ggaaatgatt
    33241 ttaaataatg gaacaacgat caaaataccg gaaaacatat aaacggtgta aaagctagca
    33301 ttgattttcg agcgagcttt gaagcgtagt tttgacaaag cgtatgcccc gctgatccca
    33361 atcactactg cagttacgga ggctatcaag gagatcacta aactattttt gaaatacatg
    33421 atgaatggaa aaatctcagc attgaaaata tcctgataat gttcaaaagt ccattctttc
    33481 ggaaaaatag tttgtgtcgt tgcaattgct tcttgtgaat ccttgaatga tgtcatcagc
    33541 atgatcaaaa atggaaacaa attgacagct aatagcaaca aaatcgtcgc gtaaaataag
    33601 attttcctgc tctttttatt tttcatttca agtccacctt ctttcgtgag agcaagacga
    33661 ccgctgcaat aatgatgaac aatagaatcg atatagctgc tgcttttcca agatcattga
    33721 acgcaaaggc tgtctgatac aaataaacgc ctaagatatt gactttattc gtcagtagat
    33781 agacatctgt aaacatatag aacatccaga tagatcgtaa aacaatcaca gttgctagta
    33841 ctggtttgat tgccggcagt gtcacgatct tgaacttctg ccaagcattt gcaccgtcca
    33901 tttcagctgc ttcataaagc gttttgtcga ctgtttgcaa aatagccaaa aatgaaataa
    33961 acgcatacgg atagtatctc cagatagcaa acaaaacgac cataaagaag ctagcaactg
    34021 gctggtcgaa ccacagcggt gcttcgttga aaagctgcaa cctatctact aacagatcat
    34081 tgacgattcc ataactatta ttgaacatat atttccaagc aaaaatcaaa gaaatagatg
    34141 gcgtaacata acttaataaa atcagtgaac gcaccgtttt cctaaatctg aacttgcggt
    34201 taagcaaaat agctacggct aatcctaaag ctgtactgcc tgctacaact aagaatgtgt
    34261 aagcaactgt taaacctaaa gaacgataaa attcactatc acttaaaatt tcaagataat
    34321 tgcttaatcc gataaaaacg gcgtctgagt taggattcag cggcatatcg aaaaaactga
    34381 tctgaatatt ggaaagcatt ggaatcagca ctaaaacagc taataaaagc atgcttggtg
    34441 ctaaaagtcc catggctaat tttaagtcag aatgtttcct tggacgattc attgaaacct
    34501 ccccctgata atctgccaat agctatctac ttttttcgaa caccattggc agaacttctt
    34561 gttatttcgt atttttttgt gcgtcagcta aagcttgatc gacatcttcg tttccgacag
    34621 ttacttggtt cactgctttt ccgatcacac ctgagcttgt gatatctccc attttcgtga
    34681 aattcttttc cccgactagt ccaaagactt gtacttcatc aaaagcacta gcaatctcag
    34741 atggcagctc gccaaaagac ttgatgactt cattttcttt gtaaccatcc agttcgacta
    34801 cttgtttatt cactggttga gcaccaccag gagacatcaa gacccacttt tccatgtttt
    34861 tcggttgaga taagaaagct gtgaatgctt cagcagcctc tttttgtttc tcgtctagac
    34921 cagaagaaat cgtcaatcca gatactgtgc catagacagc ttgatctttt tgtgttggga
    34981 tagcaaaacc gatattttct gagttccccg cttcataaac agaaggcaaa atgtaagttg
    35041 aatagattgc cattggtgct gttccattca tgaaagcatc tttgatttct gtcacatcat
    35101 tagagcctgg catcgtgtat tgggataagt tttgataaaa cgacaatgct tctctcatct
    35161 tttccgtatc gatcgttacc tcgccttcat catccaaaac atttgctcca tttgacaaag
    35221 caaattgaga gaacgcttgc tcactcattg ttccttcagc agtaggcata gcaattccat
    35281 attgtttatt ctctttatct gtgaaatgtt gagcaatttt taaaatatca tcccaatttt
    35341 ctggttctga aaaaccagct tctgataatt tttctttgtc ataccaaata ccttgaaccc
    35401 agccgctaat tggtgcagca atatagcttt ttccatcttc tgaacgaaca aggtttttgg
    35461 ctccgtcata ataattatct tctccagctt cctcgataac gttttggact gccgtctgat
    35521 caatcagttc atctttatcc atcactttag caaaatcctg actgacttcc attacttctg
    35581 gtaatttacc ggaacgagct aacgtaacga tttttgtatt gaacgctgat tcttctaccg
    35641 gcacttgctt gattttgatg tctggatttt ctttttcaaa atcagcaacc agttcattga
    35701 tcacacttaa tcgttcctgt tcgacagaag agtgcatgaa ttgaatcgtt gtctgttgtg
    35761 attcgttaga gctttgactt tcaccacaag cagtaagaag aaatccagta gctagcagac
    35821 aggtagtaac aataattttt ttcatttttt tccacctctt attttattat tttcagccac
    35881 atatattcat acggctggaa tatcaacttt cgttcttgac tgcaatggat aatttgtcca
    35941 gaaaacaggt ctaggtaatc tccttttggt aaaagataac ctgctgattg attggaaaaa
    36001 ttatggataa ctaagacttt ctcttccttc gcttcgcgaa taaaactgat aaaagaatct
    36061 gctgagtcca gtaagtaaat caaactattc ggatggaagg ctttttcttt ttttcgaata
    36121 gatagcatct gggtcaattc accatatact gctgttctta atggatctgt ctgcaactct
    36181 ttctcaatat ctgctaacaa aaatttttgc cgattgattt ttcgattctc tcctgttttt
    36241 tctacgcctt tgtaatcatt tcgacttccc agcacacttt gaatataaat tgccggcacg
    36301 cctggtaacg ttaccaaaaa tgaatgagca gctaaaaagc gtttgcttct ccattccgtc
    36361 gtatctgttg gagaacttaa cgcatctaga tacgtagcgt ttatctcata tggactggtc
    36421 gttccatctg ggtttttctt gtagtttact cgggtaccct ctttcttcag ctgttccact
    36481 aattcaagga tcgttttttc cggcagaata cctcgtaccg gattcaatcc gataccgtca
    36541 tgggaggcta aaaaattaaa aaaagttgtt tttttcgatg cgaaatcgat ggtttttaac
    36601 cattctttca acttacttgt attacccgaa cgaatcgctt gaagggtcaa aggtggtagc
    36661 gaaaactgat agaccatctg tgcttcattc tcgccattcc caaaataact gatattatct
    36721 ttgtgtggca catttgtctc agtgatcaat accgttcctg gagctgcttc atcagcgatt
    36781 gcacggaata attggatgat tttatgcgtt tctggcaggt gtatacaaga agttcctagt
    36841 tctttccata aaaatcccac agcgtccaag cgaataaatg aagcgccttt ttctaagtaa
    36901 aacaaaagaa tatcgatcat tttcaataaa acttctggat ttttaaaatt caagtctact
    36961 tgatcttcgc taaaagtcgt ccatacgtgt ttttctttcc cgtttttgtc aatgaacgtt
    37021 gtaagtaacg ggctggtcct tggtctagtc acgctgcttg tatctgtatc cagtggcaaa
    37081 tcaataaaaa aatctttgta cgtatcattc aagtttaaat attcctgaaa ccaagtactt
    37141 tcggcagaca tatgattgca gacaaagtcg aacattaacc gtgtttcgct tctcatttct
    37201 tctatatctt cccatgtgcc caaacggtcg ttgattgcct gataatttac aacggagaaa
    37261 ccatcatcag atgtatatgg gaaaaatggc aagaaatgta caactctaaa tgtatctgcc
    37321 aaatactttt gataaaattt cttaaatgta cgcaaagttg gttgctgctc ttcaaaaaat
    37381 tgatccccat aagtaatcaa gaaaatatct tcttcgtccc aatgcagttt gcctgcctgg
    37441 cctttcttct gctgataaac actgactttt tgttcaatca tagaggcggt tttctcagac
    37501 aaatctgagt cgcgataaat cgcttgtaaa agttttgtat acatcggatt cccctttctt
    37561 ttttgggagc gttcccaacg atttgatcat attatggaaa acgcttactg tcaatagcat
    37621 ttttttaaat aattttgata tacacaaaaa tacaaataag aaagcttatg tatcaataat
    37681 taatagcttc ttaaaaaaat atggtctctc atgatcatgc aagctattcc taaattttca
    37741 aaatcataga caaaaaagaa tgtctattgt actatggaat tattctaaaa cgctcccaaa
    37801 aaggaggtgc atataaaatt gaaacctact atttatgaca ttgcgaaaaa agctaatgtc
    37861 tcaaaatcaa ctgtctccag agtattaaat aaccagccaa atatctctga taaagcaaaa
    37921 gcaagtgtac ttcaagcaat caaagaattg aattatgaac cgagcaaaat tgcaagagga
    37981 ctgtcaggaa atggttttga cgcgattcta gcattatctc atcgtacttc tcatacgacc
    38041 tatggcaatc cttttttttc agatgttatt caatccattt ctgccgtttc tgaacaaaat
    38101 aacttcgact tgattctaca aacggcaaaa acaccagaag aagagctaga aaaatgcgcg
    38161 aaaaagatcc aagaaaaaat gatccgagga attattattt taagctctac gatggatgaa
    38221 acacttcttt ttgaattgga tcaatacaat attcctattg tggtaatcgg aaaagtcgga
    38281 aacgactaca cgaacatcta ctcagtggac acggataatt tccaggatag ttacgatttg
    38341 gtggaaacac tgatcaagaa tggacaccgt catatcggct gtctccatgc tccgacagaa
    38401 gtttatgtat ccaatgatcg tctcaatggt taccgtcaat gcttatttga tcaccaccta
    38461 gatatccgta atgaatggat tatagacggc ggctttcgct tagaagatag catggcagca
    38521 atgaatgatt taatgaagct acgagaaaga ccaactgcga ttttttctac cgatgcatta
    38581 aaatcattga gtatttacaa agtggcagaa caatctggaa agcagatacc tgatgatctt
    38641 tcgatcgcag gattcagtga tcccttattg tcctccctat tttcgccaca gttgactcgg
    38701 atagacattc cgacaaatca gttaggaatc aaagccacag aacttttatt ccaactaatc
    38761 caccaccaat accctgaaga aaaaagaatc attctttcaa ccgagattac tctcacagat
    38821 tcgatccaac aacaagaaat gtagaacaga attataaaag aacagaaaca ctgaggataa
    38881 gtcagttact ctgttctttt ttcaatcact catccctcgt taccccctcc cggtatttta
    38941 gcgaattttt cttattttcc atctgtttta gatagctgtg gataactata atgtttgtgt
    39001 taaactattt tttccacagg gaagaaaaaa ctatttttaa ccacgttgtg tataagtttt
    39061 tatagttcgt ttttttaaac acatttaccc ttatttcaat ccatgttatt tggggatact
    39121 tatctctaaa acataaatag aataacagac atgtctactg gttttgtttt attcgaggaa
    39181 ttattcaata atagctagca aaatattaaa aataaccgct ctatcttccc atcaatttat
    39241 tttgcattca aaaaatgatc tatctttact tttgttgatt gacttctctt ggaagatgat
    39301 cgttccgata ccttaattgg atacgaaccg atgttgttaa gatcctttcc cacttttctt
    39361 ctagttagac aaattatttt agacaaacca aaaaagaggt tgtgacaata atttgtcaca
    39421 acctcttttt taaaatatct attttgtttg attgttcatt gcgtcttctg cagccatttg
    39481 cgcttcaata tctgaaatac tgtagacatt ttcgcttgct acaccgactt cttttggttc
    39541 catgtcacga taacgttgca taccagtacc agctgggatg atcttaccta taataacatt
    39601 ttctttcaaa ccaagaagag gatctttctt accgcggatc gcagcatccg tcaagacacg
    39661 agttgtttct tggaatgatg cagcagataa gaaactattt gtttctaatg atgcttttgt
    39721 gatacctagt aagacaggtc gagaagttgc aggaacgcct cctgagacta gcgtattgta
    39781 gttttgatct ttaaagtctg cgatgtctaa taatgtacct ggtaagatat ctgtatcacc
    39841 tggatccatt acacgaactt tacgcagcat ttgacgaacc attacttcga tatgcttgtc
    39901 gccgatttct accccttgca tacggtaaac acgttgtact tcacgaagca agtagttttc
    39961 aacagataat acatcgcgaa cttgcaacaa ttgttttgga tcgattgatc cttctgtcaa
    40021 tggtgcacca cggtggatca tatcgccttc tgctactttc atacgtgctg tataaggaac
    40081 agtatacgta cgtgtatctg tttttccttt gattgttact tctttggtac gtgttgcagg
    40141 atcttctgag atatcgatga catcccctgt tacttctgtg atcactgctt gccctttcgg
    40201 attacgtgct tcaaagattt cttggacacg aggaagacct tgagtgatat cgtctccggc
    40261 aaccccacct gtatggaatg tacgcatcgt caactgagta ccaggttcac cgattgattg
    40321 agcggcgatc gtaccaactg cttctccgac ttcaacgtca gagccagttg caaggttgcg
    40381 tccgtaacaa tgtttgcaga caccatgttt tgtattacat gtgaagacag aacggattgt
    40441 cactgtttcg acacctgcat caacgatttg ttttgcgatg tcttcggaaa tcaattggtt
    40501 tcctgcaatg atcaattcat ttgtttcagg atgacgtact tctttacggg tgtaacgtcc
    40561 cactaaacga tcttctaatg attcgatgat ttcatttccc tcgcggatgg cttggatttc
    40621 aagacctcgg tcagtaccgc aatcttcttc acggatgatt acatcttggg ccacgtcaac
    40681 cagacgacga gtcaagtaac ctgaatcggc agtcttcaag gctgtatcgg tcatcccttt
    40741 acgagccccg tgagttgaga taaacatttc caagacggat agtccttcac ggaagttcga
    40801 aatgattggc aattccatga ttcgaccatt aggagcggcc atcaaaccac gcattccggc
    40861 aagctgtgta aagttggaaa tgttaccacg agctccagaa tcagacatca tgaagattgg
    40921 atttttcgct tccaaacctt ccatcagttt ttgttggatg ctgtcttttg cagcattcca
    40981 aacagcaatg acacgttcat aacgttcgtc atctgtgatc agaccacgac ggaattgttt
    41041 tgtaatagtt tcaacttgtt tatgggcatt ttcaatgatt tcttgttttt cattcagtac
    41101 actgatgtca gcgatcccta ctgtgatacc ggcatgtgtc gaatgtttgt accctaagtc
    41161 tttcatacgg tcaagcatct tagaagtttc tgttactttg aatcgtttga agacttctgc
    41221 gatgatgttt cctaagtttt tcttcttgaa cggtaatact aattcttgct ctttgatatg
    41281 tgcaggaata tcagtaccag cttcaacaaa gtatttgtcc ggtgtttgga cggtcaagtt
    41341 gaagtctgtt ggttcattca gataagggaa ttcaggaggc atgatctcgt tgaagatgat
    41401 cttaccaact gttgtgatca tcgtacgttc tttttgccat tcagtaaatg gtttttcacc
    41461 taatgagtta gggttcacac cgatacgtga atgtaaatga acatatccgt tgcgccatgc
    41521 gataacggct tcgttttgat ctctgaagac cattccttcg ccttcgcgtc cttcttcttc
    41581 cattgtcaag tagtagttac ctagaaccat atcttgggaa ggagtaacaa ctggtttacc
    41641 atctttaggg ttcaagatgt tttgagcagc taacatcaac atacgtgctt ctgcttgtgc
    41701 ttcttcattc aatggaacgt ggacagccat ttggtctcca tcgaaatcgg cattataagc
    41761 ttcacataca agtggatgca aacggattgc gcgtccttgt actaaaactg gttcgaatgc
    41821 ttggatacca agtcggtgaa gcgtaggtgc ccggttcagc aatactgggt gttccttgat
    41881 tacgtcttct aaaacatccc atacttcgtc ttcttgacgt tcgattttac gtttcgcatt
    41941 tttgatgttt gaagctagtt cgcgttgaac aagctcacgc atcacgaatg gtttgaataa
    42001 ttcaatggcc atttcttttg gaagtccaca ttggtacatc ttcaagaaag gtccaacaac
    42061 gataacggaa cgacctgagt agtcgacacg tttacctagc aagttttgac ggaaacgacc
    42121 ttgtttccct ttcaacatat gagaaagaga tttcaatggg cggttacctg gtccagtaac
    42181 tggacgtccg cgacgaccgt tatcgatcaa tgcatctact gcttcttgca gcatacgttt
    42241 ttcattttga acgatgattc caggtgcatt caagtctaac aaacgtttca aacggttatt
    42301 acggttgatt acacgacggt aaagatcatt caagtcactt gttgcaaaac gtccaccttc
    42361 taattgaacc attgggcgta aatctggtgg aataacaggg ataacgtcca taaccatcca
    42421 gcttggttga tttcctgaag cgcggaatgc ttctaaaata tccaaacggc ggattgcacg
    42481 tgtacgtttt tgtccttgtg cagatttcaa ttcttctttc aattcggctg cttctgcatc
    42541 tagatcaaca ttatcaagta attgtttaat cgcttctgca cccatagcag cttggaattc
    42601 ttgtccatat tgttcacgtt tttcgcgata ttcgcgttct gtcaacaatt gttttttctc
    42661 taaattcgta tttcctggtt cgatgactac atatgaagca aaataaatga tttcttccaa
    42721 agcgcgtggg ctcatgtcta atacaagacc catacgtgaa ggaatccctt taaagtacca
    42781 gatatgtgaa acaggagctg ctaattcaat atgtcccatg cgttcacggc gaactttcga
    42841 acgagtaact tccactccgc atcgatcaca aacgattcct ttataacgga tacgtttgta
    42901 ttttccgcag gcacattccc agtcttttgt aggaccaaaa atccgctcac agaacaaccc
    42961 ttcgcgttca ggttttaagg tacgatagtt aatcgtctca ggttttttta cttcaccgta
    43021 agaccagctt ctgatttttt cgggagaagc caaacctatt tgcatacttt cgaatttatt
    43081 tacatcgatc aaaaggggtt ccctccattt catttaatgg caaaccgcaa gacggctgat
    43141 cttcgaccgc cttgcagctc atagaccgga ttagtctaaa ttatcttctg cggtttcaaa
    43201 gttttggatg acatcgtctt tttcatcttc cacttgttca gcagctttct tttctaattc
    43261 tttggcagtt tgctgttcgg caaattttgt caaagcatca acagtgatca aatcgtcgtc
    43321 ttcatcgtcc atgtcgcgaa gttcgatctc tgtttcttcg atatccaaga cacgcatgtc
    43381 cagacctagt gattgcaatt ctttcaccaa tacgcggaat gattccggta cacctggttt
    43441 tggaattggt tcacccttga cgattgcttc gtatgttttc acacgtccga ctacgtcatc
    43501 tgatttgtat gtcaagattt cttgtaaagt ataagccgca ccgtaagctt ccagtgccca
    43561 tacttccatt tcaccaaaac gttgtccacc aaattgagct ttacctccca atggttgttg
    43621 tgtaaccaat gagtaaggtc cgattgaacg tgcatgcaat ttgtcatcga ccatgtgagc
    43681 aagtttgatc atgtacatca caccgacaga cacacgtcca tcaaatggtt cacctgtacg
    43741 tccgtcgtac agaacagttt tcgcatcgct ggccataccg gcttcacgaa ctgtttccca
    43801 tacgtcttca tcacttgcac catcaaatac tggcgttgct acgtggatac ctaactgacg
    43861 tgcagccata cccaagtgta attccaatac ttgcccgatg ttcatacgtg aaggcacccc
    43921 taaaggattc agcatgatat cgattggtgt tccatcaggt aggaatggca tatcttcttc
    43981 cggcataata cgggatacaa cccctttatt tccgtgacga ccggccattt tatccccttc
    44041 atggatttta cgtttttgaa cgatataaac acgaactaac atgtttacgc ctggtgacaa
    44101 ttcatctcca gcttcacgag taaagatttt cacatcgtga acgataccgc cgccgccatg
    44161 tggtacacgt aatgatgtat cgcggacttc acgagctttt tcgccaaaga tcgcatgcaa
    44221 tagacgttct tctgcagata attctgttac cccttttggc gtgactttcc ctacaagcaa
    44281 atcgccatct tggacttctg caccaatacg gatgataccc atttcgtcta aatcttttaa
    44341 tgcgtcttcc ccgacattag ggatttcgcg tgtgatttct tcaggtccta attttgtatc
    44401 gcgggcttct gattcatatt cttcaatatg gatagaagtg tatacgtcat cttttaccaa
    44461 acgacggctc atgatgatcg catcttcata gttgtaccct tcccaagtca tgaaaccaac
    44521 taaaacgttt tggcctagag ccatttcgcc ttgttccata gatggtccgt ctgccagagt
    44581 atcccctttt tcgactttct cacccaaatg aacgattgga cgttggttgt agcttgttcc
    44641 ggagtttgaa cgacggaatt ttgttacatc gtatttatct aatgcaccgt tgtcgcggcg
    44701 tacgcggatt tcagaagcat caacgtattc aacgacaccg tcatgtttac ataacaaagc
    44761 agctcctgag tcatgcgctg atttgtattc cataccagta ccaacccatg gtgcttgagg
    44821 attgatcaac ggcactgctt gacgctgcat gttggcaccc atcaaggcac ggttggagtc
    44881 atcgttttcc aagaaaggaa tacatgctgt cgcaacggca actacttgtt taggtgatac
    44941 gtccatgtag tctactttat caatagatac ttctaagttt tcactttgag cacgagccat
    45001 aacaacgtct tgagcaaatg ttccatcttc attcaatggg ctgtttgctt gggctacgat
    45061 gtaatggtct tcgatatcag ctgtcaagta gtcgatttgg tcagtaacac gacctgtttc
    45121 acggtctaca cgacgataag gtgtttcgat aaatccgaat ttatttactt tcgcataact
    45181 ggacaaacta ttgatcaacc cgatgtttgg gccttcagga gtttcgatcg gacacatacg
    45241 gccatagtgg gagtagtgaa cgtcacgaac ttcataaccg gcgcggtcac gagtcaatcc
    45301 gccaggtcct aaggctgaaa gacgacgttt gtgtgtcaat tcgccaagag ggtttgtttg
    45361 atccatgaac tgtgacaact gagaagaacc aaagaattct ttgatacttg ccacaactgg
    45421 acggatattg atcaattgtt gaggtgtcaa tgtttcagta tcttggatcg acattctttc
    45481 acgaaccaca cgttccatac gtgctagccc gatacggaat tggttttgta aaagttcacc
    45541 gactgaacgg atacgacgat ttcctaagtg gtcgatatca tcgacattgc cgataccttc
    45601 catcaagttc aagaaatagc tcattgaagc aatgatgtcg gctggacgaa ccgtttttac
    45661 agctgcttct ggatagccgt ttccgatcac gtttacttca cgttcagggt ctcttggtga
    45721 aaatactttg atgacttgga cagtcattgg atctgtgacc acaccgtctt ctgatggata
    45781 gtaagtcact gaatttaggc cattatcaat gaaaggagct agtgtttcca tgacttggtg
    45841 agtcagaact gttccttttt caacgatgat ctcgcctgtt tctggatcaa ctaatgtttc
    45901 agctaatgtc agattcaata gacgtgtttt caaatctaat tttttgttta ctttgtaacg
    45961 accaacgttt gctaagtcgt aacgttttgg atcgaagaaa cgagcattca gcaagttacg
    46021 tgagctgtct gctgtttttg gttcgcctgg acgcaaacgt tcgtaaacgt ctttcaaacc
    46081 ttcttctgtt cttgagtcgc ttgcgttttt atgcaagtct ttttcgactg tattgcgtaa
    46141 tgtttcgctg tcgccgaaga tttcaaagat cgtatcatcg gaaccaaatc ctaacgcacg
    46201 aactaatacg gttaaaggaa tcttacgtgt acggtcgata cgtacataag agatgtcttt
    46261 tgcatcagtt tccatttcta gccaagctcc acggttaggg ataacagtag aaccgaatcc
    46321 ttcttttccg tttttgtcga cttttccatg gaaatagaca cctggtgaac ggactaactg
    46381 agaaacgatt actcgctctg ccccgttgat gatgaatgtt ccttgttctg tcattaatgg
    46441 gaaatcaccg aagaatactt cttgtgcttt gatttcgcca gtctcgcggt ttgtcaaacg
    46501 caatgttaca tgtaatggtg cagagtagtt tgcatcatgc gcacgagctt ctgctactgt
    46561 atactttggt tctttcaatt cataatctac aaattccaaa gatagattgc cattgaagtc
    46621 atcgattggc aaaatgtctt caaacatttc tctaagtccc tcatctaaga accattggta
    46681 agagtctgtt tgaatttcga tcaaatttgg taattccaac acttcactga ttcttgcgaa
    46741 acttctacgt tcgcgatgtt ttccgtattt tactacgtgt ccagccaagc tcttcacccc
    46801 tctaaaatgt cattcaaaaa gactacccga tcaattgtta caataatatt tcaaatatta
    46861 tttttaggta acaacagaca ttttttgcgg cttttaggca aaaaaaaacc aaaaatagaa
    46921 cattctcttg aaaccttact tcgtgagctt ctacttttgt ttccttatat taaagccgaa
    46981 acggacaata tttcgtttct gccttttctt catcagatag tttttgcaac ttcatatact
    47041 acacttattt tctataaaag tcaacacttc ctggcgtttc ttctgctttt tcgcaacatt
    47101 ttcttaatgt tttcaatttt atgttttcaa ttatctgaca attcttaatt gacagacctt
    47161 tttatctcgg ttatacttta gaaaattcat tttgtggagg aacttctatg cgaaccaaca
    47221 tcaaccaaat cgcaccaaca caactgaata acttttactt tagctacttt agataggttt
    47281 gtattcatca tgatggatac aaacgttaaa cgacagtttg tatctatgat ggctaaagaa
    47341 ctttgtattg cagcaaaaag ccacgtagac aaaatgctaa gtggctgagc agtgaaaata
    47401 caaagtaaaa gtatcaagca ttctactttt tcaatttatt ttccgacttt tcaatccatt
    47461 tggacatttt gtctgaatgg attttttgtt tcccaaaatt aaggaggaaa aagaaatgaa
    47521 aaaaaatata atcatcggat taggcgctgc cctgctacta gctttaggag gttgcgcaca
    47581 aaaagacaat tccgcagagg catcagatgg acaaaaaaca attggcgtat tgcaacttgt
    47641 acaacataac tcgttagatg ctgcctataa aggattcaaa gaaggattag cagaagaagg
    47701 gtataaagaa ggcgataatc taacaatcga ttatcaaaat gcccaaaaca atcaagacaa
    47761 cctgaaaagt atgagtgaga aactcgtcaa agaagatcct gatttgctac ttggaatcgc
    47821 cactccagca gcacaatctt tattaaatga aacaaccact atcccgatga ctgtcacagc
    47881 ggtcacagac ttggaacaag cccatttagt cacatccaat gaaaaacctg gcggcaatgt
    47941 gactggcacg acagatatgg tgccaatcga aaaacaaatc ggcttactgt taaatatcgt
    48001 gccagacgcc aaaacaatcg gcatcatgta taacaacggt gaagcaaact ctaagatcca
    48061 agctgatttg gcagaaaaag aattgaaaaa agcgggcgtc aaagtcaaag tactgacagc
    48121 aaattccaca aatgatgtcc aacaagtgac gaaaagtcta gctaaagatg tagatggaat
    48181 ctatattcct actgataaca cgttcgcaag tgcggcaagt gttgtcggtg aagtcgcaaa
    48241 agaaacaaaa acacctgtcg ttgctggcgc agttgatcaa gtcaaagacg gcggtctagc
    48301 aacagtagga atcgactacg aaagcttggg taaacaaaca gggatcatgg cagcaaaaat
    48361 cttagacggc aagaatccaa gtgaacttcc tgtcgaaaca gcgaatgatt tggaactgta
    48421 tgtcaataaa gacatggcca aagcattagg catcaatcca gacagcatca aagaagctga
    48481 ataaaacaca ggaggattct catgacaaat ttgctctctc ttttcttatc atctgcatcg
    48541 caaggggtct tatgggccct acttgcgatt ggtgtatacg tcactttccg tatcttggac
    48601 attgccgact tgactgctga aggaagcttt cctttaggtg cagggatcgc ggcagtaagt
    48661 attacaaatg gctatcatcc attgacagct tgtcttctag gctttttagg gggtgcggca
    48721 gcagggcttg tttccggact gcttcacaca aaattgaaaa ttcccgccct tcttgcaggg
    48781 atcatcacca tgactggttt atattctgtc acatcccgtg tgatgggtgc gccgaacatc
    48841 tctttattgg gtcagccaac gatctttcat tgggtcgagt cattcggcat aacaaaagac
    48901 aacgctgtac tgatcgtcgg tctgcttttt gctttgatcg ttgtatgttt acttgttttg
    48961 ttcttacgta cagaaacagg acttgctatt cgtgcaactg gcgataatct ggcaatgagc
    49021 gaagcaaacg ggatcaacac agataatatg aagatcatcg gttatatgat ttctaatgga
    49081 tgcattgctc tttctggctc cttgcttgcg caaaacaacg gctttgctga tttaaattct
    49141 ggtattggaa cgatcgtaat tggacttgct tctattatca tcgcagaagt tttgttacgt
    49201 aaccagccac tatttctccg tctcttgact gtcgttttag gtgcggtcat ttatcgtttt
    49261 attttagcgt tagtcttcga actgaatgtt gaaccaagtg attcgaaact tgcttctgct
    49321 ttcgtgctag tgatctgtct ttctttcccg cagatccagc aaaaattcca tttacctcaa
    49381 aaaaagaaag gcggtagtgt caaatgaatg acgtcttaac attggaaaat ctccatcata
    49441 cctttgaaaa aggaacgatc aacgaaaatc atgtcctgaa aggcattaat ctttccttag
    49501 atcccggcga cttcgtgacg atcatcggtg gaaatggggc tggtaaatct actttgctga
    49561 acagcatcgc cggtacattt tctgtcgatc agggtcaaat tttattgaat ggaaaaaata
    49621 tcacgaaaaa atctgttgtc gaacgttcga aatccatcag tcgcgtattc caagatccta
    49681 aactagggac agccgttcgt ttaacggtag aagaaaatct tgctcttgct atgaaacgag
    49741 gaaaaaaacg cggcttcttc cgtggtgtca aaccacaaga ccgaacattt ttcaaagacc
    49801 agctggttcg gctaaatttg gggttggaaa atcgtttatc ctcagaaatc ggtttgctgt
    49861 ctggaggaca acgtcaagct attacgttat tgatggccac attacagcgt ccagaactga
    49921 tcttattaga tgaacacact gcagcgcttg accctgctac ttccaaaacg gtgatggaat
    49981 tgacagaaaa gctgatcact gagcagaatc tcactgcttt tatggtcact catgacatgg
    50041 aagacgcgat ccgctatggg agtcgactga tcatgctgca tcaaggaagg atcgtcgtag
    50101 atatccagca agaggaaaaa cagcagctca cagtgcccca attattagcg ttgttcaaac
    50161 aaaacagcgg cacggcatta aaagacgatc aagtactttt atcttcataa tcagaaagag
    50221 gatgggacaa aaaatgaacg cccaccctct tcttttttct ctttccttct ttcgtttttg
    50281 ctttatgtat caaccgttgt ttcttcgaaa actattttgt tctctccttc ttcctgatag
    50341 aatggatggg aaaggagctt gatgccatga caacgaaaga caaggtactt caattattga
    50401 aaaagacacc agattttctt tccggagaaa agttggcaca gcaactggaa gtttcccgga
    50461 caagtatttg gaaagccatc aaagaattgg aaaaggcagg ctatcgtttt gagcacgggc
    50521 ctacagggta tcgttacctg ccctctgacg tactagatgc aaaagaaatc cagcaagagc
    50581 tacggtcgtt tattcctgat ttgaaagtac aaacgatgtt gacttctgaa tctacgatga
    50641 aagatgtcaa actggcagca gctcaaggag attctgtttc gaagttattt gttgctgata
    50701 cacaagaaca agctcgtgga cgttttggac ggcctttttt cgctgaacct ggacgaggaa
    50761 tctacatgag tctcttgctc catccagaca aacggtttga tgaattgcct caatatacga
    50821 tcatcgcagc cagtgcctgt gtgaaagcca ttgaatctgt aaccggtcgt tctgcttcga
    50881 tcaaatgggt caatgatatc tacatggatg gaaaaaaagt ctgtgggatt ttatctgaag
    50941 cagtcagtga tatggaaagc ggcaggatca gtcatatcat cattgggatc gggcttaatt
    51001 tttctgttcc tcaaaatcat tttccagctg atctccaaga aaaagccatc tctgtctttc
    51061 ccgacggaca agctagtgtc acaaggaatc agctaatcag caaaatctgg caagaattgt
    51121 tcacttatct ttctcaccca gaaaaagcgt tggacttcta taaagaaaaa tcattcgtat
    51181 tgggaaaaac cgtgtcgttc aatcaaaaag gaacgactta tgtcggtctt gcaaaagaaa
    51241 tcggtgatca tggcgaactg atcgtccaaa caaatgaaca gaagatgagt ttgtcttctg
    51301 gcgaaatcag tctcacatct atccgtacgc aataattatc agatttcaca aaaggacacc
    51361 tgatctcata cagtgtcctt ttttctgaat tctcttcaaa agactctcct tattctatca
    51421 gctgacagtt tctctatcct ctagaaaaag gtattctaaa aatatctata aagcatggta
    51481 cactttcttt aaatgaatca atgcaaaaaa caagagagtc atcagtttac acgatcaaaa
    51541 gagatagcag gccgcttcct ttttcaatct tatagataga ccatgggtaa cgataaaaac
    51601 aaaaaagaac ttgcgactta ttttgccgta agttcttttt tagctagtgc cttaatctct
    51661 taatcccaac aaacgtctcg catagatcac ctgaccagag tgcatagcag catcatcaat
    51721 aatagaaacg agacgcattc ctcgtgtgac tggtggtgtc cagttctcgt caattacttc
    51781 atctagctcg gaaggttcca gccttcttaa gtaagaaata gcaaaatcag cagctcctcg
    51841 caagtatcct agcagatctt ctatttgatt agcttggatc tgctttgctt gttccagact
    51901 atgcttccaa tcttgttcgt tatctgctat cgtcatcgga aaccggcttt cccagtcttg
    51961 actatgccaa attggagaag ttcctgctaa atcagcaatc tggaaatcta attctcgagc
    52021 ggtatgccaa gccagccaag tcattgattt gatctgcggg gctctatctt ccactggaaa
    52081 acgattggcc tcctcaatag agacgccttc aaacaaacgt tcaaaccgat cgtatgcccg
    52141 atccacactc tcgcatagca tatctaaata atccatttat tttcctcctt tttatttatc
    52201 ctacttactt tttcctaatt ctaggatacc tacttatatg ttactacaca cccttatttt
    52261 ttacacttta acagtcacca tcatcaatag tttggagatt gaaagaaaaa gatagtgtcg
    52321 aactatcaat tgaacaagaa gcaacaatat gaacgtttca ttaatattct ttaaataaac
    52381 aatgattttt caaacgaaca gtttaagact aggagaaaaa gagtataata ggaaaatgtt
    52441 tgaaaggaaa ggaaggggaa aaatgaaaaa aagggcaagt ttactagtcc tagttgcttt
    52501 gttgttcaat atcttttcgt atggtgtggc cgtttcagcg gaaacataca ttcctaaaga
    52561 ccctgggaat atttataatg atctaactgg taactatggc ttgttaggaa ttgctagtca
    52621 attccacgta tttgctaaaa ataagacaac tgtgaatgca catacagacg gaaatatagc
    52681 aacacatgaa cttgatgcaa aaaataattt tggtacagag cttcatgaag gtttattgaa
    52741 ccaagaaata aattatatac aaaaaactga ttctttcgct aattcttcag gcatacctac
    52801 aggtagcaca agaatgaata aatttgtcgt tggcagtaac gtggatgtag cacttcaaga
    52861 tggtcatcct acagtcaatg gatccaggct tgatcactta actccagatg atttttataa
    52921 agatcgtgct gggcatgaat atattgattt cgagcaggaa tttgctaaat tgaatgtagc
    52981 agcaaatgat cttgcaacga tcactccagc taaaacatat actgctgcag attttccaga
    53041 catgaacaat cggacaatcg atttaactga tttatcagat accgggtttt tattggtaaa
    53101 tatcgatgca gaagtgttaa cgatgaatac gccacttcaa atcattaatc caaatgatca
    53161 agtagttgta tttaatgtca tcaacagttc ttctgcattg aacgtacagt caccaatcaa
    53221 atacaatgat cgttcaaatc atgaaacaga agatttttcg gatgcgaata tctcttggaa
    53281 ttttggaaat gagatgactg atttgacaat cagtgctcct tttcaaggaa caatccttgc
    53341 gcctaatgct actatccgca ttactcaaaa ccaagacgga acgattatag gaacaaatgt
    53401 gattttagat gcagctacca atcgttggga tcctaacgaa atatttatca gcgacaatgg
    53461 tacagatacg acagacacaa gcgacagtac agatacatct accacaagtg atagctcaga
    53521 ttcatcaact tcaagcgaca gtacagatac tacctctacc acaagtgata gcacggatac
    53581 atcagcttca agcgacagta cagatactac ctctaccaca agtgatagca cggacacatc
    53641 agcttcaagt gacag
//
LOCUS       DAZKLM010000006        51226 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3764
            SAMEA115706913-rid22582563.denovo.006, whole genome shotgun
            sequence.
ACCESSION   DAZKLM010000006 DAZKLM010000000
VERSION     DAZKLM010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706913
            Sequence Read Archive: ERR13196647
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 51226)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 51226)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287823.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287823.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,004
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,849
            CDSs (with protein)               :: 2,849
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 41 of 96
            Pseudo Genes (incomplete)         :: 52 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 15 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..51226
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706913-rid22582563.denovo.006"
                     /isolate="VRE3764"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            330..1175
                     /locus_tag="ACG842_000311"
     CDS             330..1175
                     /locus_tag="ACG842_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFQ0246881.1"
                     /translation="MDEMKLIGIDLDGTLLNSDQSISQKNARTMKHLPEDCFPFICSG
                     REVEDINNILKKSGLRIPAVGLNGSVGFDGEKKIFESSFDKNDVKKIDSVLSKFPTKI
                     YTNLGSYESQNYKNKMKKIFHEVGSEFSIKELNYELEYEKSICSIPYNNIEEIIEKDA
                     ISIYKFFIFIPNRQLKANIFNHLKKMLNVSVTESAPVNIEIVPQNVSKGVVFDRLESI
                     YNLKKPLRIAIGDSLNDLSMFESADISFAMGNSHQEIMKKADYVTVSNDKDGVSQAFE
                     RILTI"
     gene            1456..2676
                     /locus_tag="ACG842_000312"
     CDS             1456..2676
                     /locus_tag="ACG842_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HFQ0246882.1"
                     /translation="MVKFIHAADLHMDRSFEGLTTLDKTVQEKLLKTNLTVLSNIIEQ
                     AIINNVDFVLLAGDNFHQNRPSLKIQKHFSEQMKRLEKNHIPVFIIFGNHDFYQKERY
                     WFSFPKNVHLFTSETVETKKITIKSGETVSLSGFSYRQPWIQKDKVMEFPSRELTDYH
                     IGLYHGEPGVSQKGNYAPFQPSKMQEKGYDYWALGHIHVPTVLNEKQTIVYPGAPQGH
                     TKKEQASTSILLVELSKGSCQIHPIKVAEVYWKTKEISLRKARTTKEVIVHIRQQLSK
                     VEDGFSLIEIKLKDYDHLNTDVLERIQSGELLDYLLEEFSDRINDFFIWRISLIENTF
                     YTKTPLAASAKLMEQLFQYYQTPQDFQQILNEVYSHQEAARILSELPEYQEETLEKAK
                     QLLNQDFLFEEDQE"
     gene            2673..5393
                     /locus_tag="ACG842_000313"
     CDS             2673..5393
                     /locus_tag="ACG842_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFQ0246883.1"
                     /translation="MKILRIEIAAFGKWRQKSFDFYSGNQLIYGGNEAGKSTIYQFIQ
                     AILFGFPSKGRKKKDYTPKDGSAYGGKIWLKHPVYGEFAVERYKQQNRGKSKVWLGDQ
                     VGSDELLEKLIAPLTKELFQQVFTFQQEQLMELDHLREKELHDALISLGITGSSQVFQ
                     KRQEYYQKAQQLFKKKGQKLLINQKLNEWKELQEKIHQKQEQEAYFGKLIQQEQEYES
                     QQQQLLAQLKEANKQFSLARQQELNFSLYEEWQSLKKNSTTGLPDEQLLLDLESFSKR
                     YQQTNERLEELEATLEKHSGMDQQSARYYFYLEQEQELKDLQQLQFTAEQLNEEWQRL
                     QIETRETNSELKILEQRWHWQKNPLPEEFLYEEEWKHLLAEERRIADLESQISVRLQI
                     AKEQQEMIEAEVNDLEETYPALLQNQSEIAPVSSSKPYMLMAGGAIIIAGIFVPMPLK
                     VILLLIGAGMIGYFFYKRQPKIKTKNDFSEIKGMWQEKLGQLDLALAQLAEAEEKQKR
                     TGEEKQILQQEVYRFADHRHLGKMDTLKLMKEYGQEVIDYQKLYGVFERKHKREQEIQ
                     EKLRQIDQKFEFVKEWLPIQDKTISEKMVLLSRFIQEMEQMKFARSYQQNTLLKQEIN
                     QLKKKQKVWIQEYSEQLTQAGLSYPSEVPVFLQKAKQAEQQMKRYNELSEMLTPLFPE
                     ELTKEKLSVRLRQLKEKQSQLQQEEKEASENRQRLQLQVEELQRDGTLDELYQQASRQ
                     KAKLQELLEEWGALEIAGTFLGDLSTEMSERQLPQLLRAAGDYLGILTKGNYQKILLK
                     NDTLTLTDGYTEFPIYELSTGTKDQLIMAMRFAYLSLEANRSICPIIIDDGWLHYDHQ
                     RKYQLAELLAKFGEKYQIICFSSDKEMVSYYHEMNQIVKQLEGANNEKNS"
     gene            5377..6321
                     /locus_tag="ACG842_000314"
     CDS             5377..6321
                     /locus_tag="ACG842_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFQ0246884.1"
                     /translation="MKKIRDLVIDEQFEQFVLIKSADVRIAKNGKKFIAFTFQDTSGT
                     IDGKYWDASEEEINKFTAGTVVFLNGKREVYQGNPQVKILHMRLTRVDEPGEPSLYME
                     RAPLKKEEMEEEINQLLFEITNAHWNRIVRYLLNKYQKQFFEFPAAKRNHHAFASGLA
                     YHTLTMLHLGKSIADEYPELNRALLYAGIILHDLGKVIELSGPMSTEYTLAGNLIGHL
                     VLIDEEITKACLALKISEADEDVLVLRHMVLAHHGLLEYGSPVRPRIMEAEILHQIDN
                     IDASMQMMLTSIRQTEPGKYTDRIFGMDNRSFYVPKDL"
     gene            complement(6390..7400)
                     /locus_tag="ACG842_000315"
     CDS             complement(6390..7400)
                     /locus_tag="ACG842_000315"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFQ0246885.1"
                     /translation="MKKKSIILAATSALAVLTLAACSGDTNKDIATMKGGTITVSDFY
                     DEAKLESSNQSLVQRMIIYKVFNNKYGDKVTDKQVDAEYDKQAKSLGDTFESQLEAAG
                     YTKDTYKEYIRNNLAFEAGLKAHVDITDDDLKTAWKSFHPEVEAQIIKLSSEDEAKDV
                     KKSADDGDDFSKLAKDKSTDTETKEDGGKVKFDSTTTTIPAEVKEAAFKLKDGEISDV
                     ITTTNPTSYATEYYVVKMVKNQNKGNDMDKYKDQLKDIATETKLSDNAFTTKVIGEEL
                     KDANVKIKDDAFENVLSAFTTTSSSTKDSSETTASTKSSDTKSTDSTKESSTEETTDS
                     SK"
     gene            complement(7580..7924)
                     /locus_tag="ACG842_000316"
     CDS             complement(7580..7924)
                     /locus_tag="ACG842_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HFQ0246886.1"
                     /translation="MIKNFFKGLLFGAAAGTVGGLLLAPRSGNETRKKLIEELEEATT
                     LTNDLNNSLNHFKSALIETKETAESVIPAFQEAIKKDIEDFKFQAEPRITQINEQVEK
                     LKADLPEYPEQI"
     gene            complement(7917..8351)
                     /locus_tag="ACG842_000317"
     CDS             complement(7917..8351)
                     /locus_tag="ACG842_000317"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HFQ0246887.1"
                     /translation="MENCIFCKIINQEIPSYKIYEDDKVYAFLDISQATKGHTLVVPK
                     RHVADIFEYDPELAGEVFSRVPKIAQALEKAFPEMKGLNILNNNRELAYQSVFHSHIH
                     LVPRYSKEDDFSIHFVNHQDSYGSEELKAIQETIVKQVSCDD"
     gene            8489..9226
                     /locus_tag="ACG842_000318"
     CDS             8489..9226
                     /locus_tag="ACG842_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0246888.1"
                     /translation="MSLIIDHVTGGYGHLPVLKDISFDVQPGEIVGLIGLNGAGKSTT
                     IKHIIGLLEASKGTIEINHLSLKKNTEEYRKQIGYIPETPSLYEELTLREHIEVTAMA
                     YDIPMDIAMKRAEKLLETFRLSNRLDWFPANFSKGMKQKVMILCAFLIEPSLYIIDEP
                     FLGLDPLAINALLELMVEMKKEGAGILMSTHILATAEKYCDRFVVLHNGEIRADGSLE
                     MLRKEFQLPDSSLDEIYIALTKEQAHE"
     gene            9219..10430
                     /locus_tag="ACG842_000319"
     CDS             9219..10430
                     /locus_tag="ACG842_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0246889.1"
                     /translation="MSSFFQKRLARHQKKMMKYMRYVLNDHFVLVCLFLIGGVGLYYS
                     NWLKTLQPPFAIGGLIISVFWMLCLFVGKMATFAEAADIVFLLPKEKEMQTYLERAFR
                     YSCIFPLIFLILACGLAMPLVVVSTEQSFSMFFVYLIILWSLKFSHLQIKRAELFRLD
                     QQVIRYWKLGWFFSSLIFLFLSFYWLIWAGIAGALVQASLYYWFLWKQQEKGLDWEKM
                     VHVEEHRLHQIYQFINLFTDVPEITAKIKRRRYLDPILTHIKRESKNTYLYLFARRLA
                     RGSDFSGLYFRLVIIGGLLIAGVVDFYFSLIIGFLFLYLIGFQLLPLANQFRYMVLVQ
                     LYPIHKNQKKQAIQYLLTWVLIIASVIFGLIAAVVLDGQQRWVPLAAYLIVTMLFVKL
                     YVPTRLKKMSD"
     gene            10634..11416
                     /locus_tag="ACG842_000320"
     CDS             10634..11416
                     /locus_tag="ACG842_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HFQ0246890.1"
                     /translation="MMEFQLDQEWRLRPIKGDTGKTYIGMRDEDKVFIKRNTTPMLAA
                     LSREGITPKLVWTKRTGNGDTLTAQEWLEGRLLTPNEIGQRNDVIDVLYHLHHSNSLK
                     TMLTRIGGRVMTPEKMLQKYEKELPEELQKNQFLQIVHRFLREQIPSIDKENLSVVHG
                     DVNHRNWLVCKNYLYLVDWDSIMFADPAVDIATILGNYVPLSSWNQWLISYGIRPTDE
                     MLEKFHWYAAMNLLQEITRYCKREDHRRMNEEILQLKRIFSG"
     gene            11486..12148
                     /gene="trmB"
                     /locus_tag="ACG842_000321"
     CDS             11486..12148
                     /gene="trmB"
                     /locus_tag="ACG842_000321"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine(46)-N7)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HFQ0246891.1"
                     /translation="MRVRNRPGAAEMLAAHPNFVISDPTLWKGKWNELFENDHPIHIE
                     IGMGKGQFITGMAKAHPEINYIGVEMQVSVVSIALDKLIEQPLPNLKLLHVDGSALTE
                     YFADSEVDQIYLNFSDPWPKKRHEKRRLTYKTFLAVDEQILRPNGEIHFKTDNQGLFE
                     YSLASFSQYGMILKQVWLDLHQSQFEGNIMTEYEEKFSSKGQRIYRVEAQFQDKTKKK
                     SL"
     gene            complement(12246..14000)
                     /gene="ade"
                     /locus_tag="ACG842_000322"
     CDS             complement(12246..14000)
                     /gene="ade"
                     /locus_tag="ACG842_000322"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000034 - adenine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006146 - adenine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HFQ0246892.1"
                     /translation="METTLRKYIEVASKKKPADLVIKHAKIVNVFTKEIMEKDVAICE
                     GMIVGIGEYEGKSVHNANGQYLVPGFIDGHVHIESSLLSPSEFSKVSLLHGVTTVVTD
                     PHEIGNIAGSTGLEFMIEDARQTLMNIFFMLPSCVPVTPFETNGANLNAKKLAPFLQK
                     PEVLGLAEVMNYQAVANNETDILEKIQLMHQHKKKIDGHAAGIGMEELNVYPAAGIRT
                     DHEATTAKEAKERLDLGMYLMVREGTVAKDLASLLPAITPENSRRCFFVTDDKLINDL
                     VKEGSIDHIVRQAIALGIDPLQAIQMATLNAAECFDLKTLGAIAPGYQADFFLTDDLT
                     KLPIHEVFYKGTLVVQSGQLQDHLFADHSNSYTCCLPKLNAQPLNPHSLELLLSSSQA
                     HVIEIIPNSLVTKDLVEKVDRQGERFIPSIEKDQLKIAVIERHHQTGNIGLGIVKGFQ
                     LKQGAIATTIAHDSHNLVVVGTNDEDMLYAANLLIQKGGGMTVVNKQQEIACVSLPIG
                     GIMTQEPFSIVYEQLNELITKAHQLGAAKTFDPFLTLSFLTLSVIPELKITDKGLFSF
                     SRFQLIPPCGKTDEINTF"
     gene            14132..15061
                     /gene="comGA"
                     /locus_tag="ACG842_000323"
     CDS             14132..15061
                     /gene="comGA"
                     /locus_tag="ACG842_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus ATPase ComGA"
                     /protein_id="HFQ0246893.1"
                     /translation="MEIKRLSDQMISESFAKHAQDVYILPASEVFYIYFRYGKKRTFW
                     KKVSSADGQQLISRFKYLGQMDIGEKRKAQLGAFTYSLEKQEVRLRLSSVGDYLQRES
                     LVIRLLYGTFETPHRCFSSRDFATILQKTVRRGLYLFSGPVGSGKTSLMYRLALEEKL
                     QVITIEDPVEIEEPFFLQLQINPKIAQTYDTLLRLALRHRPDLLIIGEIRDESTAHAA
                     VRAALTGHRVFATVHARNLEGTLVRIHELTKKKEELSECLAGVIYQELLEDANHHPSV
                     LWSYQFMEEESVKKSWEESYEEAQENIWQSELF"
     gene            15018..16067
                     /gene="comGB"
                     /locus_tag="ACG842_000324"
     CDS             15018..16067
                     /gene="comGB"
                     /locus_tag="ACG842_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus assembly protein ComGB"
                     /protein_id="HFQ0246894.1"
                     /translation="MKKLRKIFGSQNYSKGLTRSQQNQFAQTMGELLLSGFSLQESLY
                     LLLTIRSFPPVILANIQHQLKEGQAFSEVLSQIGYGAEQLVQIELAERHGNLPTTLLE
                     IAKQFRLVEGFRNDLKKLLAYPMLLLTFLLGILFSMRLFVLPQLIGSGMVMQEHWGIQ
                     LIQVFHWYVLLFILAVTLLALLVRYRLMKLSPVDQSEWISRQLLIGPIYSLYQSAYLS
                     LEIGKLFHEGFELRQIIVCLKETKQESLIQHLASKLIQGLEAGMSLAEQFQNYRFLSE
                     EFSKIVLQGEAKGSLGKELIFYSEWTRKQLFKRLHHWMHFIQPIIFLGVAALILLVYA
                     AVLLPVYGSIEEVLP"
     gene            16064..16342
                     /gene="comGC"
                     /locus_tag="ACG842_000325"
     CDS             16064..16342
                     /gene="comGC"
                     /locus_tag="ACG842_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus major pilin ComGC"
                     /protein_id="HFQ0246895.1"
                     /translation="MKKRKISYYSGFTLIEMLIVLLIISVLVLLFVPNLSRYRNHVDQ
                     ESREAIIQLVDTQKELYALQNNGRVPTVEELLNEGYIKREHAEIYQRP"
     gene            16317..16787
                     /gene="comGD"
                     /locus_tag="ACG842_000326"
     CDS             16317..16787
                     /gene="comGD"
                     /locus_tag="ACG842_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus minor pilin ComGD"
                     /protein_id="HFQ0246896.1"
                     /translation="MQKSISGLEGKVLASGYTLFESLLLILLLTTILSFPVLAFSAWK
                     NELAVYQFFGQFEKRIYSTQKIAIVNQIQTGFYWNSEENQIIFHVPNPDKINWEVLDI
                     PDEITLKRHASITFAARTGNESSLKAYQFYWEEKERTITYQFQIGSGRYTKRIE"
     gene            16759..17106
                     /locus_tag="ACG842_000327"
     CDS             16759..17106
                     /locus_tag="ACG842_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0246897.1"
                     /translation="MVAIQKELNKTGFVLLESLTALMIASVCVYLVSFGQLFLLQAEK
                     KSGEELLFIRMIYEDIKTQRMYHKRPERREEMIPYDIQIVDNNGVIEKVFVNMEKEAI
                     SIEKTSNSSIYAD"
     gene            17069..17494
                     /gene="comGF"
                     /locus_tag="ACG842_000328"
     CDS             17069..17494
                     /gene="comGF"
                     /locus_tag="ACG842_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus minor pilin ComGF"
                     /protein_id="HFQ0246898.1"
                     /translation="MKKQVIPAFTLIECLIGLMILSSLLLTFNLLIQQTIQVSEFLQR
                     KDQKEWLIFLAQLEEELKSSHNVSVKNQQLHYTIEDKQYIIERYQAMIRKRRTSGGHQ
                     PMLTSITELQLLEKEHTISFYVHFENGEDGYAIWTKNDQ"
     gene            17466..17825
                     /gene="comGG"
                     /locus_tag="ACG842_000329"
     CDS             17466..17825
                     /gene="comGG"
                     /locus_tag="ACG842_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus minor pilin ComGG"
                     /protein_id="HFQ0246899.1"
                     /translation="MPYGQKTISRKCYKGSILFSVLLLFLLCTFLLMAALESYRLSMD
                     LNIRIKNYYIAKIMKQMALSEQSTLAESSEGVFYYTTGSVTYQWVQQSLFLEVEVSPF
                     IFRFTEEVKNDTDTSTE"
     gene            17997..19004
                     /locus_tag="ACG842_000330"
     CDS             17997..19004
                     /locus_tag="ACG842_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFQ0246900.1"
                     /translation="MLPEKMEQGFNQNLEAIQLLQNALGTSFLEAYVENAENLIDDYQ
                     VRVVDGVPTKETTQRITALYEELKKLSFEPEEWRRLSQLLLLKGSQTEHLQANHQLTP
                     DSIGFLFVFLIEQLYTNKKAPVKILDIAAGMGNLLLTVLLNLSNAGYQTEGIGVDIDD
                     TLLAVAASTSDLTQANVQYFHQDGLQELLIDPVDFAISDLPIGYYPNDEKAKEFLTST
                     EEGHSYAHHLLLEQSMKYVKPDGFGLFLMPSGFLETDQSEEIKKWFKEEGYLQGMIQL
                     PDELFRNKQSQKSILILQKKGPQAQQVKEVLLVKLASLKEPEKVTEFFNEFKNWKSSA
                     L"
     gene            19029..20213
                     /locus_tag="ACG842_000331"
     CDS             19029..20213
                     /locus_tag="ACG842_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate/propionate family kinase"
                     /protein_id="HFQ0246901.1"
                     /translation="MSKTIAINAGSSSLKWQLYQMPEETVVAKGIVERIGLQDSIFTI
                     KYGDNQKFEQIMDIENHEVAVKMLLDQLIELQILSSYDEITGVGHRVVHGGETYGDSV
                     VIDEKVMDRIAELAEFAPLHNPANLMGIKAFKKILPDILSVAVFDTSFHSTMPKQNYL
                     YSLPVSYYEDFKVRKYGFHGTSHRYVSERAAKLLNKPIEELKIITCHLGNGVSITAVD
                     GGKSMDTSMGFTPLAGVTMGTRSGDIDPAVLPYLMEKLEIDIDEMINVLNKKSGLLGL
                     TGLSSDMRDLEKNYEKEHIRLAYDIFVDRIRKYIGGYVTTMNGVDAIVFTAGIGENDG
                     HVRSEIIKGMSWFGCEIDPALNELRGEEVDISTKESKVRVLVIPTDEELMIARDVERL
                     RG"
     gene            complement(20650..22233)
                     /gene="abc-f"
                     /locus_tag="ACG842_000332"
     CDS             complement(20650..22233)
                     /gene="abc-f"
                     /locus_tag="ACG842_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protection-like ABC-F family protein"
                     /protein_id="HFQ0246902.1"
                     /translation="MIIQAISIKKTIGGNPLFEDLSCQIEAGEKIALVGINGTGKSTF
                     LQILTGREGVDSGVISRKKGLKIGTVEQELTVSEATVNHYLLHSAAEIQDLKQQMSRY
                     EALMTEPDTNLEKCLARYGELQHRFEELGGYVLEDRIAAILQGLGIPHRQEARLTELS
                     GGERVKVALAKILAADADLLLLDEPTNHLDLNSIRWLENYLQNTKKSVLVVSHDRQFL
                     DQVTTKTWELEEGALIEYPGNYSRFRVLKEARLAELTKNYELQQKEVQRLKVMIRRFR
                     QWAHEGDNESFFKKAKELERRLAKLTLVKPPPPPKNRLQSLSNGGKSGKEVFIIQNLH
                     QQYADQVLFKDSSFAVYRGDHLAIIGDNGAGKSTLLKLLLGEEKPYGGTIKLGSSLQI
                     GYLPQQLQFADPDSRLLAYAITLTGNEEVARRQLAKSGFYQSDVGKRIKDLSGGEKIR
                     LALLKLFLEKINVLILDEPTNHLDSYAREEIEEMLQDYQGTLLAVSHDRYFLQQHFQE
                     ALVIDQEQISRQPLGQLID"
     gene            complement(22338..22466)
                     /locus_tag="ACG842_000333"
     CDS             complement(22338..22466)
                     /locus_tag="ACG842_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0246903.1"
                     /translation="MMRMTKITSNENYQRYHIAFSHAIGFTVAVDGTSLSKTVEFT"
     gene            complement(22811..22954)
                     /locus_tag="ACG842_000334"
     CDS             complement(22811..22954)
                     /locus_tag="ACG842_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HFQ0246904.1"
                     /translation="MEVSDESDTKKYEVSMADTDFIAKLKFYFGFGYREKASATFGKS
                     FKN"
     gene            complement(23177..23656)
                     /locus_tag="ACG842_000335"
     CDS             complement(23177..23656)
                     /locus_tag="ACG842_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HFQ0246905.1"
                     /translation="MLQQYKKIMVAVDGSDEAELAFKKAVNVAIRNNGELLLAHVIDT
                     RSFQTVSSFDGMLAEQATEMAKQTLADYESNAKKAGLNNVTSVVEYGSPKQIIAREIP
                     EDNQVDLIMLGATGLNAVERLFIGSVSEYVIRNAACDVLVVRTDLENQLPAKDHTKA"
     gene            complement(23729..24013)
                     /locus_tag="ACG842_000336"
     CDS             complement(23729..24013)
                     /locus_tag="ACG842_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0246906.1"
                     /translation="MIRILMIIATLVLLFVSYYLFNKQDIFFVLINKNDKNQGFLQFY
                     GAAYAVLGVMGILTAFFNQRFIALVFLLIVIIVSATFSIRFAKKIAEPKQ"
     gene            complement(24091..24285)
                     /gene="ssrS"
                     /locus_tag="ACG842_000337"
     ncRNA           complement(24091..24285)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="ACG842_000337"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(24357..25643)
                     /locus_tag="ACG842_000338"
     CDS             complement(24357..25643)
                     /locus_tag="ACG842_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HFQ0246907.1"
                     /translation="MQQPLAYRMRPRTIDEVVGQQHLVGEGKIIRRMVDARMLSSMIL
                     YGPPGTGKTSIASAIAGSTNYAFRMLNAATDSKKDLQVVAEEAKMSGTVILLLDEVHR
                     LDKTKQDFLLPHLESGRIILIGATTENPYITINPAIRSRTQIFEVKPLTEQDIQLAVE
                     HALRDKERGLGQQAIQLDEDALLHLSRATNGDLRSALNGLELATLSTPSDKEGRIHLT
                     LSIIEECVQRKALTHDKNGDAHYDVISAFQKSIRGSDVDAALHYLARLVEAGDLASIC
                     RRLMVIGYEDIGLGNPAAAARTVNAVLAAEKLGLPEARIPLADVVVDLCLSPKSNSAY
                     MALDAALADIREGKAGDVPDHLRDSHYKGAKSLNRGVGYQYPHHFDQAWVNQQYLPDK
                     LKNAQYYQPKDTGKYEQALGQQYYRIKEWKKHPPKN"
     gene            25781..26452
                     /locus_tag="ACG842_000339"
     CDS             25781..26452
                     /locus_tag="ACG842_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HFQ0246908.1"
                     /translation="MTDKQKIDEFFWNKTTPEVARDLLGMYLEHDTPEGRLAGYIVDA
                     EAYLGPEDEAAHSYGLRRTPRVRAMYEKPGTIYLYTMHTHRILNIITQPEGIPQGVMI
                     RAIEPAAMIDQMSKNRGGKTGPDISNGPGKLVEALAIPQELYGQSIADSSLRLVFEKK
                     KTPKKINALPRIGIPNKGVWTEKPLRFVVSGNPYISLQRKNQIEKDWGWRKENEKEGS
                     INIFR"
     gene            26418..26906
                     /locus_tag="ACG842_000340"
     CDS             26418..26906
                     /locus_tag="ACG842_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3013 family protein"
                     /protein_id="HFQ0246909.1"
                     /translation="MKKKDLLTYLDEIIEKQVTDYDVALDWDTKNHTIEIVVRLFAEN
                     KAQFEIDDAEGIVSQEPIIEFEDGVLLFNPQKSVFDERNYLAVIPYEGKKGLAKSVAD
                     SLVEYLNEVLAQGQSDLLDFLDEDDDEAVFELHWDNQQLAELVEVKQQNEADTYLPYP
                     SY"
     gene            26919..27866
                     /gene="prmA"
                     /locus_tag="ACG842_000341"
     CDS             26919..27866
                     /gene="prmA"
                     /locus_tag="ACG842_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HFQ0246910.1"
                     /translation="MKWNEVKVETESEAVEAVANILMEAGASGVAIEDSLDVEHFQSD
                     QYGEILDKNDFTSLKEGALVMAYFPETTFLPEILPFIKDRITQLPEFGLAIGKNKVTV
                     SEVEESNWATAWKKYYHPVRITRMLTVAPSWENYQTEDPLEKIITLDPGMAFGTGTHP
                     TTSLTLTALETVLRGGETLLDVGTGSGVLSIAAKYLGAKDVYAFDLDEVAVRSAKENM
                     DMNEIAKDVHVSANDLLTGIEIESDVIVANILADIILLMIPDAWRLLKQTGTLIVSGI
                     IEEKKQMVLDAMLEQGFIVDQIFQQKDWYAIILKKPEVE"
     gene            27867..28631
                     /locus_tag="ACG842_000342"
     CDS             27867..28631
                     /locus_tag="ACG842_000342"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HFQ0246911.1"
                     /translation="MQRYFLKESYESKDYFVLEGENYHHIVRVMRMEPEDEVFLAFND
                     QIAIVAEITEITEEAVYLKEIKKEENQKELPSSITIASGYPKGEKLDWIVQKSTELGA
                     QAFLGFPASASVVKWDAKKRMKRQQRLEKIAQEAAEQSHRQIVPSVELLETKQDLFER
                     LDDYDVVLVAYEESAKQGEKSNAAKILSETKPDSRILAVFGPEGGLSRDEVDYFVEHK
                     GILCGLGPRILRTETAPLYLLSAASYQWELMNPSFS"
     gene            28864..31077
                     /locus_tag="ACG842_000343"
     CDS             28864..31077
                     /locus_tag="ACG842_000343"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016616266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HFQ0246912.1"
                     /translation="MTKEEILTGPGVIKLVSQYMGPEHVAFVEKACEYATAAHDGQFR
                     KSGEPYIIHPIQVAGILADLKMDPHTVATGFLHDVVEDTEITLEDLREEFGDDVAMLV
                     DGVTKLGKIKYKSHEEQLAENHRKMLLAMAQDLRVIMVKLADRLHNMRTLKHLREDKQ
                     RRIAQETLEIYAPLAHRLGISRIKWELEDTALRYLNPKQYYRIVHLMQTKREEREKYV
                     SGTVEDIRIATEELGIFAEIYGRPKHIYSIYRKMKDQKKQFNEIYDLLAIRVIVDSIK
                     DCYAVLGAIHTKWKPMPGRFKDYIAMPKANMYQSLHTTVIGPAGNPVEIQIRTQEMHE
                     IAEFGVAAHWAYKEGKNEKVEPDGMTKQLSWFHEILELQDESYDASEFMEGVKGDIFS
                     DKVYVFTPKGDVTELPKGSGPLDFAYSIHTDIGNKTTGAKVNGKMVQLDYKLKNGDII
                     EIMTSPNSFGPSRDWLKLVATSKARNKIKRFFKAQDREENVIKGHESVVKCITDLGFT
                     PKDILTKNKLQEALDRFNYQTEDDLYAAVGYGEVSPLTMANRLTEKERKEQKIEQQKQ
                     EAEEIMNQPKKEPDKMKVRHEGGVVIQGVENLLIRISRCCNPIPGDDIVGYITKGRGI
                     SIHRRDCPNVQPDKPNVAERLIEVEWEDTSNTRKEYDADLEIYGYNRSGLLNDVLQTV
                     NALTKNLNSVEARTNKDKMATIHLTVGIQNLSHLKSIVDKIKAVPDVYSVRRTNG"
     gene            31088..31534
                     /gene="dtd"
                     /locus_tag="ACG842_000344"
     CDS             31088..31534
                     /gene="dtd"
                     /locus_tag="ACG842_000344"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HFQ0246913.1"
                     /translation="MRAVVQRVSKAAVSVDQQEIGKIGYGLLVLLGIHETDTQKDVDY
                     LVKKIAQMRIFEDEQGKMNISVEDVGGQILSISQFTLFADTKKGNRPSFVEAARPETA
                     IPLYESFNEGIRSRGITVATGQFGADMSVSLVNDGPVTIIIDSKNK"
     gene            31705..32964
                     /locus_tag="ACG842_000345"
     CDS             31705..32964
                     /locus_tag="ACG842_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HFQ0246914.1"
                     /translation="MKEVPVVDIKITDLKKLYQKGYNMLEELRHEADAPVVKAKIFNK
                     EAITIYGSSAAKVFYDPRNFKRKGAMPKLVLKTLFGQGGVQTLDGAAHHHRKNIFMDL
                     MTPERMEDYHRILDKNLTQALEAQHGQFELFDLSKMVFFTSICEWAGINLSAISKDEV
                     EKLAEYQISMISGTFTSPIDHIKGVENRKKSEKWAQGLIEEARQNPVAGKENVALYAF
                     ANATDLDGQLLPLEVAAVELLNIIRPTVALTVWAALMGHALFSRPDLYQQLKNDFSTL
                     QDPFIQEMRRYYPFFPMLPAISLKEVEVDGYRIPEGSWVILDLYGTDHDERTVEAPDS
                     FMIKRYVGKAKDISYKEEYEMIAQGGGNFRQMHRCAGEWITLHSLRVFSDQLVNKFEF
                     SVPEQDWTIPFNQFPTYPNSRALLYKN"
     gene            complement(33053..34000)
                     /locus_tag="ACG842_000346"
     CDS             complement(33053..34000)
                     /locus_tag="ACG842_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFQ0246915.1"
                     /translation="MAEIRIGHSQVYAEQLGLGANAVGGHNLFDGLEDETGKQVVRTA
                     LNSGINLIDTAYAYGNGRSEELIGEVLKEKEYDRSRVVIATKAAHVPNKGRTFDNSPE
                     FLKQSVEDALKRLQTDYIDIFYIHFPDESTPKNESVATLHELKEAGKIRAVGVSNFTL
                     EQLKEANADGYVDVVEDKYSLIHRQAEKELFPYLEKNKISFVPYFPLASGLLTGKYEL
                     GEEKQFGEGDPRKRNPDFQGERFREILTAVDVLRPIAKRYQATPAQLVLAWYMKNPRV
                     SVVIPGAKRPEQVSDNVQALDLHLSNEDYQTIDEAFRGF"
     gene            34394..35686
                     /gene="hisS"
                     /locus_tag="ACG842_000347"
     CDS             34394..35686
                     /gene="hisS"
                     /locus_tag="ACG842_000347"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HFQ0246916.1"
                     /translation="MSFQRPKGTNDILPGESEKWQFVEETARLLFRDYQYNEMRTPIF
                     EHYEVISRSVGDTTDIVSKEMYDFYDKGERHVTLRPEGTAPIVRSFVEHKLFGPEYAK
                     PFKTFYMGPMFRYERPQKGRLRQFHQIGVEAFGSENPATDVESMVMALDFFKQLGITQ
                     IRLVINSLGDKETRQAYRQALIDYLLPFESELSEDSKRRLHENPLRVLDSKDKRDQKF
                     VAEAPSILDYLSEPAQKHFDTVVSMLDALAVPYEIDSNMVRGLDYYTHTIFEIMSDAP
                     KMGAQATICAGGRYDNLVEELGGPSTPGFGFAMGIERLLLTMEAEEVVIPAFNELDAY
                     VVALGDETNIEALKVVQAIRNFGFSADRDFMNRKAKAQFKTADKANAKLVLVIGEDEL
                     ASQTINVKAMASRKEKSFPLEEIYEHFDEVYDEMTLFE"
     gene            35701..37470
                     /gene="aspS"
                     /locus_tag="ACG842_000348"
     CDS             35701..37470
                     /gene="aspS"
                     /locus_tag="ACG842_000348"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HFQ0246917.1"
                     /translation="MAQRTIYCGLVSEQQLEQTVTLKGWVQKRRDLGGVIFIDLRDRE
                     GIVQIVFNPEINKEAWEIADKCRSEYVIEVTGEVRRRGENAVNPKMATGEFEVIASEI
                     VILNQAKTPPFQIEDDQAISDEIRLKYRYLDLRRPKMTKNIKLRNATTKAIRHYLDDN
                     DFLDIETPYLGKSTPEGARDYLVPSRVHAGHFYALPQSPQLFKQLLMNAGFDRYYQIV
                     RCFRDEDLRGDRQPEFTQVDIETTFLTAEEIQTYTEGLIAKVMKEVRGIEVTLPFPRM
                     TYDEAMARYGSDKPDTRFDMELIDLSDTVKEVEFKVFQMALENGGVVKALNAKGAADR
                     YSRKDMDQLGQYVGQFGAKGLAWLKVEEDGLKGPIAKFMGEATEAIIKATDAKPGDLL
                     MFGADKSEIVAAALGAIRTRLGKELGLIDESKFNFLWVTDWPQFEFSEEEGRYVSAHH
                     PFTMPKEEDIPLLAEDPAKVYAEAYDIVLNGYELGGGSIRIHTRELQEKVLETLGFTK
                     EQMEEQFGFLLTALDYGFPPHGGIALGLDRFAMLLAGEDNIREVIAFPKNGKAADVMS
                     DAPSMVSPLQLFELNIDVTNIEE"
     gene            37579..38454
                     /locus_tag="ACG842_000349"
     CDS             37579..38454
                     /locus_tag="ACG842_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFQ0246918.1"
                     /translation="MKKTFENWAYHDYTTKLSVSIVYALIASVALNFFYQPGHIYSSG
                     ITGLAQILTTLSKSLIGFEVPVSTTLYLLNIPLFVLAWLKIGKKFTIFTFITVTLTSL
                     CIHILPETVLSTDPIICALFGGAVMGLGIGLALRNGLSSGGLDIVSITIRKKTGKSIG
                     SISIYFNALIVFVAGILFGWQYMFYSTLSIFVSGKVTDVVYTKQKKMQVMIVTKHPEK
                     VVNEIQNCMRRGITIINEAEGAYKHDKQTVLMTIVTRYELPLLREAMKASDPQAFVSI
                     AENVQILGRFYEDGI"
     gene            38720..40603
                     /locus_tag="ACG842_000350"
     CDS             38720..40603
                     /locus_tag="ACG842_000350"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HFQ0246919.1"
                     /translation="MEKQKRLKNSRYITGFDGLRSLAVVGVIFYHLLPTSMKGGYLGV
                     PIFFVVSGYLITDLLRQEWEQNGKINIWQFYVRRMKRLYPALVFMLVTASAYITLFQR
                     GLLNNLRGVVVSSLLYVNNWWQINNGLSYFDRFANESPFTHIWSLAVEGQNYLVWPIL
                     FVLLMVFVRKRKWIFYTVLGTSFLSAILMMILYTPGGDPTRVYYGTDTRLFSIWLGSA
                     LAFVWPSTRLKKNIPKQAKNILNIAGGISLLALILCFFFLDDHYNFIYYGGMLLVSLL
                     CVVLVAVTAHPGASLNRWLTNPVFTWIGKRSYGIYLYQFPVMIFYEAKVTDIADHVLL
                     HTLVEITLILIISELSYRYVERPLARFDYGRTFQVMKGWFSKPIISKSKPWVVPGTLL
                     VAIALVGFVTAPKNAVTADQKQLQAKIAESKKLAEETRNNSENTTSSVDQAILDKYEL
                     SKAQGNKAQKLELTAFGDSVMLDAATDLQELYPKVVVDGDVGRQLYASVPYIEKLKEQ
                     DLLKDTVLIGLGTNGSFSEAQFDSVMDAIGDRKVYWVNVRVPTKRWQNDVNAMLEQMT
                     KKYDNMTLIDWYSYSNDHSDWFYDDQVHPNPEGMTHYIHLVSEALLGGSSSTNSSETN
                     VTE"
     gene            complement(40678..40962)
                     /locus_tag="ACG842_000351"
     CDS             complement(40678..40962)
                     /locus_tag="ACG842_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HFQ0246920.1"
                     /translation="MANEHYFYVLFCKDQTFYGGYTTDLSRRLEEHNNGTGAKYTRLQ
                     KRRPVKMIHAEVFSSRSEATKAEAAFKKLTRKQKERYLQTHSTVLLPESI"
     gene            complement(40952..41686)
                     /locus_tag="ACG842_000352"
     CDS             complement(40952..41686)
                     /locus_tag="ACG842_000352"
                     /EC_number="2.1.1.223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016430 - tRNA
                     (adenine-N6)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HFQ0246921.1"
                     /translation="MLHEDERIDQLYAEDIKIIQSSKVFSFSLDAVLLAHFSRVPKKG
                     KIVDLCAGNGAVGLFLSKRTQAAIDAIELQPRLADMARRSIALNHLNEQMTVHTIDLK
                     DSLSVVRHNSCDLVVCNPPYFKGLPTNKTNPNKHLAIARHEIHTTLEEVIDISSKLLK
                     TNGRFTMVHRPDRFLEIIQVMEDYRIAPKRIQFVYPKPGKEANILLVEGIKEGKLDGF
                     KVAPPLITYNMDGEYTAEIREMLYGK"
     gene            41815..42438
                     /locus_tag="ACG842_000353"
     CDS             41815..42438
                     /locus_tag="ACG842_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HFQ0246922.1"
                     /translation="MFYRFMRGVVRVLLALVNGNARYENKEVLPQDENYILVAPHRTW
                     WDPLYLAVAAAPKEFSFMAKEELFKNPVLRFILTRSNAFPVKRENPGPSVIKTPVKIL
                     KSTNLSLIMFPSGTRHATELKGGMALISRMSKAKIVPAVYQGPLTLGDLFKRQRVTVR
                     FGEPIDLSDIKKTDKEGMAEIERRTQAAFDKLDKEVNPDFKYEVKKK"
     gene            42630..43751
                     /locus_tag="ACG842_000354"
     CDS             42630..43751
                     /locus_tag="ACG842_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HFQ0246923.1"
                     /translation="MRFLHTADWHIGKKLQGYDLIEDQRHVLSSILDIAKEEKVEAIV
                     IAGDLYDRSVPAVDAVELYNELMVDWNLKEKIPIFAISGNHDSSTRLSAGTPWFSYSN
                     YFLRTQLAEAFEPVEFKNVQFFFLPYFEPIAARLYFEDDNIRTIKQAVAKVIDKMQEL
                     FDSEKKQVLISHFFVMGSVKSDSETKIEVGGLDSIPGELLTPFDYVALGHLHNQAALR
                     QDNARYSGSPLKFSLSEINQKKGVWIVDLTTGLELNFREIEPLHDIEEITASFKELLD
                     PAFYQSVNRENYIYIYLTDRTIIPNMMNQLRQIYPRILGIERANGRAEQTKRKRKVLE
                     MKDPQKLAEAFFSEMTEEQLTANQEKWLGETLQEIMEME"
     gene            43758..46886
                     /locus_tag="ACG842_000355"
     CDS             43758..46886
                     /locus_tag="ACG842_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFQ0246924.1"
                     /translation="MKPKKLQLKNFGPFINETVDFSRLTEAPLFLISGKTGAGKTTIF
                     DGITFALFGETSGRLRSGKEMRSLFATPDEETSVTFSFEHQQMSYEIERKPEQVLAKR
                     KGNGTKKQAAKVTLTIFDGKGKEIKQITKRTEADQLIRELMNLDAKQFSQIVLLPQGE
                     FRNFLVSSSSDKETVLRHLFGTQFFQQFNERLKEKAKQQQQKLDHLEQELHLLQKRFV
                     LVKEEEPAAGFEAVLAQWTNHQAILEKQITEEKEALTELQEKQKQLENSYYRFESLKK
                     SYEEKTILINKQKELAAQKDEIEEKKRWIGYYEFTQRLVEPIQRLQEIKVEKIELSQK
                     SSEQTKKLAMTTQNYEEWQKNEANRVRRQTKIKEAAQKLQNKQMMVPIVESLRSKKQD
                     AEQLTISLDKEELNAEKLYQNLAIYQERTRILQENLLDQEKLQEDRLAFGQLKYLDEK
                     QQEIEKQKAKDTGSLAQIREELKKATEEITKNSQLLKEQTEQAEQLKSQWAKQQIARL
                     QLFLRPGEPCLVCGSTEHPIQQLKHEQPNKEDILKVEQQLAEAESAVERTQQIIAKSE
                     AHENNLQQQLSDLEQKIMATNIQLAEQEEHLQQALKKAFPEITEQKTKTTLVVIEQSL
                     DRRKSKMANARIELEEIRQQAEKLEQQLAVYKQQQTEKNNQLQQLQGEIKSLEQQLQD
                     TDSNCLEEEIRRLEKQLEEDSNQLAVEQKAGVQLLQDLHTLRTQLDLQHEQIEKIDEK
                     IEKEQERIAKKLESQSYFSTIEEICAFAQQQAQYKENQRTVTSYHEEQLVTADRLRQL
                     ENIQLTETFPDTEEISQQLEEIRRMIEIKQEKIYGLQEQKISNQKIYDECLSIYHTSQ
                     TKLDELTQLQQLSQTFNGENPKKTSLERYVLQVYLQEVLQVANDHLQRLTKNRYQFEL
                     ADTIGSYRGKTGLEINIYDDQAGMSRSAHTLSGGESFIAALSLALALADVIQTQAGGV
                     AIDALFIDEGFGSLDEEALEMAMEALETIESEGRMIGIISHVRELKERVLQQIRIDTE
                     GSGQSKIRYQLG"
     gene            46905..50099
                     /locus_tag="ACG842_000356"
     CDS             46905..50099
                     /locus_tag="ACG842_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2 helicase associated domain-containing
                     protein"
                     /protein_id="HFQ0246925.1"
                     /translation="MKWSIPEKVIARGRQYLQEGRVLSVVPDQEENVWHAEVLGSELY
                     MVDLDGTAKEIDYCQCPYWEEHKYCKHTVAVELYLRSKNLSRIMKKDQPMLEPVKATS
                     EGEIIAKGFSRLKTQNNPQAVQPLIIEYQVETIETNQYHPELSILALYLRIGSLASPK
                     KTYIVKNIYDFLQAYTEKETYTVNKQYQFRLDKRAFQKEDQKILTHLAGSAQTHQLLG
                     TNGLQVKGKLDKRYLLLPVEQARFLLEQMNQTTRLHLQINEQNDQGIQFSDQNKPLSF
                     KVEKFGEDYLLQALNDFDFFFMHYQWGVIQGTFYSLTKYQQTVYQTWKQLIRRLEKPE
                     VVFKKKELPSLFKEIIPLLSEIGDVAIAPAVEEEVADIPVEFIFFFRKAKGKIQARVD
                     FVYGEVIYSTDPKHEVSTEQAFQVLRDTVEEKRVLDLFRMYRYQKNETGYERPLPSGE
                     ELYAFFRTELSVFRQFGEVRLGRKLRELYLDAHKHQPKIEVTETGSWLDIRFDVTGIE
                     EQEIDHVLQSLLRNDAFYTLENGQVLSFDSEEFQQTSQVLQQLRESIRTEEGTIHVPK
                     NQGLIIQNQLEKSNATFSESFQTMVQDLIHPERYQAQLPKGLNATMRDYQKQGFRWLK
                     MLGHYQFGGILADEMGLGKTLQTIAFLLSEKEERKSFSALIVAPASLIYNWQAEVRKF
                     APSLSIQVINGNKKEREELLAKDTDIRVTSYASLRQDLANYQSQKIDYLILDEAQMVK
                     NSSTKTAQALRELAVPQRFALSGTPIENNLEELWSLFATIMPGFFPTKTKFRELAAEE
                     IAQMIRPFILRRDKQTVLKDLPEKTEMNLYSALTEEQKTVYLAYLRQMREEITSMDSE
                     AFKKNRIGILAGLTRLRQICCDPRLFIEDYQGGSGKLEQVKDLLVAAKENKRRVLLFS
                     QFTGMLTILQEELAELGISTFYLRGSTKPQDRLSMVDAFNAGEKDVFLISLKAGGTGL
                     NLTGADTVILYDLWWNPAIEEQAAGRAHRIGQKNVVEVWRMISEGTIEERMDSLQQEK
                     RELFQKVIQGNEEQLTKLTEEDIRMILSVGEIEE"
     gene            complement(50245..50952)
                     /locus_tag="ACG842_000357"
     CDS             complement(50245..50952)
                     /locus_tag="ACG842_000357"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFQ0246926.1"
                     /translation="MIKSVVFDVDDTMYDQQQPFRNAVKRVVPLVSDADMHPLYIRFR
                     HHSDENFPKVMAGDWTLEYMRAHRISQSLKDLDYPHITEEDGLLFQKIYEEELDNICL
                     HEEVKKTLDFLKEKNVPLGIITNGPTDHQTKKLKQLQLNNWIPSRNMLISQATGFQKP
                     EKEIFQLAEKEFHMLPEETLYVGDNYDNDVLGAKSANWQALWFNHRERKIEQTPICDI
                     EISSFDQLLETMKTIFV"
BASE COUNT        16763 a         8946 c        10638 g        14879 t
ORIGIN      
        1 aaggcaaaaa gatgaactag aaatagttca tctttttttg tttaaatctt ttgctaagtt
       61 gaactaatag agtcttttat gaatatcgaa gttagttttt cacctaactt cgttttttat
      121 agttggttat tataaaatag taatatagct gatttggatt caattcatat ttatttataa
      181 atcaaaaata cagagaatag tatataaatg tcctttccta ttcttaaaat gatgtttgta
      241 cttacttaaa ttaggtataa ttagtatatg ttttgtgaga aatttagact tacaataagc
      301 agtgaattta taatcaacgt ataggagaca tggatgaaat gaaactaatt ggtatagatt
      361 tagacggtac tcttttgaat tctgaccaga gcattagtca aaaaaatgca agaacaatga
      421 aacatttacc ggaagactgt tttccattca tttgttctgg aagagaagta gaagacatta
      481 ataatattct caaaaaatct ggtttaagga taccagcagt agggttaaat ggttctgttg
      541 gatttgatgg agaaaaaaag atatttgagt cttccttcga taaaaatgat gtaaaaaaaa
      601 ttgattctgt cctatcgaaa tttccaacca aaatatatac aaatctcgga agttatgaat
      661 ctcagaatta taaaaataaa atgaaaaaaa tttttcacga ggtagggagt gaattttcta
      721 tcaaagagct aaactatgag cttgagtatg aaaaatctat ttgttcaatc ccttataata
      781 atattgagga aattatagaa aaggatgcta tcagcattta caaatttttt atttttattc
      841 ctaatcgtca attgaaagct aatatattta accatttgaa aaagatgcta aatgtgtctg
      901 ttactgaatc ggcacctgta aatattgaaa tcgtacctca aaatgtatct aaaggtgtgg
      961 tgtttgatcg tttggaatca atctacaatt taaagaaacc tttgagaatc gcgatcggag
     1021 atagtttaaa tgacttatct atgtttgaaa gtgcagatat tagttttgca atgggaaata
     1081 gtcatcaaga aatcatgaaa aaagctgatt atgtcactgt ttcaaatgat aaagacggag
     1141 tatctcaagc atttgaaaga attcttacca tataaactaa ttaaggtagt atagaacata
     1201 gaattctaaa tacggaaaaa tggagcataa attccagtac aaatagtact tcttcagtgt
     1261 caaacgaagc gaatataaca tagatattta ggattgtgac aaaaatcatg tcacaatctt
     1321 tttttcgaat aaacttattt tttgaagtcg gccatttatc gtaatttctt tgcataatac
     1381 gaacaaattt tcggttattg atgaaaaaaa acattgaaaa tggtatgata aaagaaaaaa
     1441 aggaggaaat ttttaatggt gaaatttatc catgcggcag atttacatat ggatcggtca
     1501 tttgaaggac tgacaactct tgataaaact gtacaggaaa aactattgaa gacgaatctg
     1561 accgttttat caaatatcat tgaacaggca ataataaata acgttgattt tgttttactt
     1621 gcaggagata attttcatca aaatcgtcct tcattaaaaa tacagaaaca tttttcagaa
     1681 cagatgaaac ggttagaaaa gaaccatata cctgtattta taatattcgg aaatcatgat
     1741 ttctatcaaa aagagcggta ttggttctcc tttcctaaaa atgtccattt atttacatcc
     1801 gaaacggtag agacaaaaaa aataacgata aaaagtggag aaactgtctc tctcagtgga
     1861 tttagttatc gtcaaccgtg gattcaaaaa gacaaagtga tggaatttcc ttctagagaa
     1921 ctcacagatt accatattgg gttgtaccac ggggaacctg gcgtctctca aaaaggaaac
     1981 tatgctcctt tccaaccctc aaagatgcaa gaaaagggat acgattattg ggctttaggg
     2041 catatccatg tgccaacggt cttaaatgaa aagcaaacaa ttgtttatcc aggtgcgcct
     2101 caaggtcata ctaaaaagga acaagcttct acatctattc tactagttga attaagtaaa
     2161 ggaagttgcc aaatacatcc gattaaagta gcagaggtct attggaagac aaaggaaata
     2221 tcattgagga aagcaagaac gacaaaagag gtcattgttc acattcgtca gcagctttca
     2281 aaagtagagg atggatttag tttaattgag ataaaattaa aagattatga ccatctcaat
     2341 acagatgttt tggaacggat acaatcagga gagctattag actatttgtt agaggaattc
     2401 tcagatagga taaatgattt ctttatttgg cgtatttctc tcatagagaa tactttttat
     2461 acgaagaccc cacttgctgc ttctgctaaa ttgatggaac agttatttca atactatcag
     2521 acacctcaag atttccaaca aattttaaat gaagtatata gtcatcaaga agcggctaga
     2581 atattgagcg aactgccgga atatcaagaa gaaacacttg aaaaagccaa gcagctgctt
     2641 aatcaagatt ttttatttga ggaggatcag gaatgaagat tttaaggatc gagattgctg
     2701 cttttggaaa atggcgtcaa aaaagttttg acttttattc aggcaatcag ctgatctatg
     2761 ggggaaacga agcaggaaaa tcaacgattt atcaatttat tcaagcaata ttatttggtt
     2821 tcccttcaaa aggccgtaaa aaaaaggatt atacaccaaa agacggctca gcctatggcg
     2881 gcaaaatatg gcttaaacat cctgtatatg gtgaatttgc tgtcgagcgc tacaagcagc
     2941 aaaatcgcgg aaaatcgaag gtgtggctgg gggatcaagt gggaagtgat gagttactag
     3001 aaaaactgat cgcacctttg acgaaagagt tattccagca ggtatttact ttccagcaag
     3061 agcagttgat ggaactagat catttgagag aaaaagagct acatgatgcg ctgatttctt
     3121 taggcatcac gggcagttct caagtattcc aaaagcggca agaatattat cagaaagcac
     3181 aacaattatt caagaaaaaa ggccaaaaat tactaatcaa tcaaaaatta aatgaatgga
     3241 aagagctgca agaaaaaatt catcaaaaac aagaacagga agcttatttt ggcaagttga
     3301 tccaacaaga acaggaatac gagtcgcaac agcagcagtt attggcacaa ttaaaagagg
     3361 cgaacaaaca attttcatta gccagacagc aagaacttaa tttttcttta tatgaagaat
     3421 ggcaatctct caaaaaaaac agcactacag gtcttcctga tgagcaactt cttttagatc
     3481 tggagtcttt ctccaaacgg tatcagcaaa caaatgagcg tttggaagaa ttagaagcaa
     3541 cactggaaaa gcatagcggg atggatcagc agtcagccag atattatttt tatttagagc
     3601 aggagcagga actaaaagat ctccagcaac ttcaatttac agcggaacaa ttaaacgaag
     3661 aatggcaaag attacaaatt gagactagag aaacaaattc agaattgaag attttggaac
     3721 aaaggtggca ttggcagaaa aatccgttac cggaagaatt tttatatgaa gaagaatgga
     3781 agcatcttct ggctgaagaa agaaggattg ctgatctaga aagccagatt tctgtgcgtc
     3841 tccaaatagc taaagaacag caagagatga ttgaagcaga agtgaatgac ttggaagaga
     3901 cctatccagc attattacaa aatcagtcag agatagcacc agtctcttca tctaaaccgt
     3961 atatgctgat ggctggcgga gcaataatca ttgcaggtat atttgtacca atgcccttga
     4021 aagtgatcct tttactaatt ggtgcaggca tgattggcta tttcttctac aaaaggcaac
     4081 caaaaattaa aactaaaaat gatttctctg agataaaagg aatgtggcaa gaaaaactag
     4141 gacaacttga tctagcctta gctcaattag ctgaagcaga agaaaaacaa aagagaactg
     4201 gagaagaaaa acagattctt cagcaagaag tatatcgttt tgcggatcat cggcacttag
     4261 gaaagatgga tacgctcaaa ctcatgaaag aatatggaca ggaagtcata gattatcaga
     4321 aattatatgg cgtatttgag cggaaacata aaagagaaca ggaaatacaa gaaaaactac
     4381 gacaaatcga ccaaaaattt gagtttgtta aagaatggtt accgatacaa gataagacaa
     4441 tttcagaaaa aatggtattg ctttcccgtt ttatacaaga gatggaacag atgaaatttg
     4501 ccagaagcta tcagcaaaat actttattga agcaagaaat caatcaattg aaaaagaaac
     4561 aaaaagtatg gatacaagaa tacagcgaac aattgactca agcaggctta tcttatcctt
     4621 cggaagtacc tgtcttttta caaaaagcca aacaagccga acagcagatg aaaagataca
     4681 atgaattatc ggaaatgctt actccactat ttcctgaaga gctgacaaaa gagaaattat
     4741 ctgtaagact gcgccaactg aaagaaaagc aatcccaact ccagcaagaa gaaaaagaag
     4801 caagtgaaaa tcggcaacga ctccaacttc aagtagaaga gttgcaaaga gacggcacat
     4861 tggacgaact ttatcaacaa gctagccggc aaaaagcaaa attacaggaa ttactggagg
     4921 aatggggagc acttgaaatc gccgggacat tcttaggtga tctttcaaca gagatgagtg
     4981 aaagacaatt accccagttg ttaagagcag caggtgatta tttaggtatt ttaacaaaag
     5041 gaaattatca gaagatatta ctgaaaaatg atacactgac tttaacagac ggatatacag
     5101 aatttcccat ctatgagtta tctacaggta cgaaagatca gctgattatg gcgatgagat
     5161 ttgcttacct ctcattagaa gctaaccgtt cgatatgccc gatcatcatt gatgatggct
     5221 ggcttcatta tgatcatcag cgaaagtatc aattagctga acttttagct aaatttggag
     5281 aaaagtatca aataatttgt ttttcttctg ataaagaaat ggtaagctat taccatgaga
     5341 tgaaccaaat cgtaaaacaa ttagaaggag caaataatga aaaaaattcg tgatttagta
     5401 atcgatgagc agtttgaaca atttgtcctg atcaaaagtg cagatgtaag aatcgctaaa
     5461 aacggaaaaa aatttattgc ttttactttc caagatactt caggtactat cgatggtaaa
     5521 tactgggatg cttcggagga ggaaatcaat aaatttacag ccggcacagt cgtttttcta
     5581 aacggtaaaa gagaggttta tcaaggaaac cctcaagtaa agattcttca tatgcgctta
     5641 acaagggtag atgagccagg agagcctagt ttatacatgg aaagagcccc tttgaagaaa
     5701 gaagaaatgg aagaagaaat caatcaattg ttgtttgaga tcaccaatgc gcattggaat
     5761 cgtattgttc gctatctact taataaatat caaaaacaat tttttgagtt tccggcagca
     5821 aaaagaaacc atcatgcatt tgccagtgga ttagcttatc atactttgac aatgcttcac
     5881 ttagggaaat caatcgccga tgaatatcct gagctgaatc gagcattgtt gtatgctggt
     5941 atcattttgc atgatttagg aaaggtgatt gaactttccg gtcctatgtc gacagaatat
     6001 acgcttgcag gaaatttgat cggtcatctg gtattgattg acgaagaaat aactaaagcc
     6061 tgtcttgcgt tgaaaatcag cgaagctgac gaagatgttc tcgtattacg tcatatggtt
     6121 ttagcacacc atggcttatt agaatatggc tcacctgtac gtccaagaat aatggaagca
     6181 gaaatattac atcaaatcga caatatcgat gcttcgatgc agatgatgtt gacttcgatt
     6241 cgtcaaacag aaccaggaaa atacaccgat cggattttcg gtatggacaa ccgaagtttc
     6301 tatgtaccaa aagatttata gcaaaaattt atttttttca ttcaaaacaa aaaaagcgag
     6361 acataagtct tcgctttttt tgttttcttt tattttgatg aatcagttgt ttcttcagtt
     6421 gaagattctt ttgttgaatc agttgatttt gtgtcagaac tctttgttga agctgtagtt
     6481 tcacttgagt ctttcgtact tgatgaagtt gttgtgaatg cactcaacac attttcaaag
     6541 gcatcatctt tgatttttac atttgcatct tttaattctt caccgattac tttagttgtg
     6601 aatgcattgt cacttagttt agtttcagta gcgatatctt ttagttgatc tttgtattta
     6661 tccatgtcat tgcctttatt ttggtttttc accattttga caacgtagta ttctgtcgca
     6721 taagaagtag gatttgttgt agtaataaca tcggagatct caccgtcttt caatttgaaa
     6781 gctgcttctt ttacttctgc aggaatagtt gttgtcgttg agtcgaattt aactttaccg
     6841 ccatcttctt ttgtctctgt atcagttgat ttgtctttgg ctaatttaga gaaatcatct
     6901 ccatcatccg ctgatttttt cacatctttt gcttcatctt cacttgataa tttgatgatt
     6961 tgtgcttcta cttctggatg gaaagatttc caagcagttt tcaaatcgtc atctgtaata
     7021 tctacgtgag ctttcaagcc agcttcaaaa gctaaattgt tacgaatata ttctttataa
     7081 gtatctttcg tatatccagc agcttctaat tgtgattcaa aagtatcacc taatgatttc
     7141 gcttgtttgt cgtattctgc atccacttgc ttatcggtta ctttatcgcc atatttgtta
     7201 ttgaatactt tataaatgat catccgttgt actaaggatt ggttcgatga ttcaagtttt
     7261 gcttcatcat agaaatcaga tacagtgatc gttccgcctt tcatcgttgc gatatcttta
     7321 ttagtatcgc ctgaacaagc tgctagagtt aaaacagcca acgcactagt agctgccaaa
     7381 ataattgatt tttttttcat taaaatttgc actcctgtaa ttagttatta tttttatata
     7441 ttaactatac catatcccat ttcctttaaa aacgttgtcg tggtagtttc aaacaaaatt
     7501 caaaatttca tcacattcct aggaaatacg caaacttttc tcacttatat agttaaatct
     7561 gttgagaaaa taatcattat tatatttgtt caggatactc tggcagatca gcttttagtt
     7621 tttctacttg ttcattgatt tgtgtgatac gtggttcggc ttggaattta aaatcttcga
     7681 tatctttttt gattgcttct tggaaagcag gaatcacaga ttcagctgtt tcttttgttt
     7741 cgatcaatgc gcttttaaaa tgattcaggc tgttatttaa atcatttgtc agagttgttg
     7801 cttcttccaa ttcttcgatc agtttctttc ttgtttcatt cccgctcctg ggcgccaata
     7861 acagacctcc gacagtgcca gcagcagctc caaataacag tcctttgaaa aagtttttaa
     7921 tcatcgcagg acacctgctt tacgattgtt tcttgaatag cttttagttc ttccgatcca
     7981 taactatctt gatgattgac aaagtgaata gaaaaatcat cctctttgct gtagcgaggg
     8041 actaaatgga tatgtgaatg aaaaacagat tgataggcta gttcacggtt gttattcaag
     8101 atattaagac ctttcatttc tggaaaagct ttttccaatg cttgagcaat cttaggcaca
     8161 cgcgaaaaaa cctctcctgc aagttcggga tcatattcaa aaatatctgc tacatgacgt
     8221 ttaggcacaa ctaatgtgtg tcctttcgtt gcttgagaga tgtctaaaaa agcatagacc
     8281 ttgtcgtctt catatatttt atagcttggg atttcttgat tgatgatttt gcaaaaaata
     8341 cagttttcca tataaaacat cctttctata aagaataaat acttcagctt aactttacca
     8401 taagttaaag ctattgtaag gaaaaaaaca aaataccgtc attttcttcg tggtataata
     8461 aataaaaata aatggcggag gaaaaagaat gagtttaatt atcgatcatg tcacaggggg
     8521 gtacggacat ctccctgtgt taaaagatat cagtttcgat gtacagcctg gagagatagt
     8581 agggttgatt ggactgaacg gagcagggaa aagtacgacc atcaagcaca ttatcggtct
     8641 gctggaagct tcaaaaggaa cgatagaaat caatcatcta tccttaaaga aaaatacgga
     8701 agaatatcgc aaacaaattg ggtatattcc agagactcct tctttatatg aagaattgac
     8761 attgagagaa catatcgaag tcactgcgat ggcctacgat atacctatgg atatagcaat
     8821 gaaaagagca gaaaagttgt tggagacttt tcggttatct aatcgtcttg actggttccc
     8881 agctaatttt tcaaagggaa tgaaacagaa agtgatgatc ttatgcgcct ttttgatcga
     8941 gccttcttta tatatcatcg acgaaccatt tttaggcttg gatccactag ctatcaatgc
     9001 attgcttgaa ttgatggtag aaatgaaaaa agaaggagcc ggtattttga tgtctacgca
     9061 tattttagct actgcagaaa aatattgtga tcgttttgtg gtcttacata acggagagat
     9121 acgagcagac ggttctcttg agatgttgag aaaagaattt cagctgccgg attcttcttt
     9181 ggacgaaatc tatattgctt taacaaagga gcaggcacat gagtagtttt tttcaaaaac
     9241 gtttagcaag acatcaaaag aaaatgatga aatatatgcg ttatgtattg aacgatcatt
     9301 ttgttttagt ttgtcttttc ttgattggcg gtgtgggatt atattattct aattggttga
     9361 aaaccctgca gccaccgttt gcaattggag ggctcataat ctctgtattt tggatgctct
     9421 gtttattcgt gggtaagatg gctacttttg cagaggcagc agatattgtt tttttacttc
     9481 ctaaagaaaa agaaatgcaa acctatttgg aacgagcttt tcgctattct tgcatttttc
     9541 cgttgatctt tttgatattg gcatgcggat tggccatgcc attagtggtg gttagtacgg
     9601 agcaatcctt ttcgatgttt ttcgtctatc tgatcatatt atggagtttg aaattcagtc
     9661 atctgcaaat caagagagca gaattgtttc gactagatca acaagttatt cgttattgga
     9721 aattaggctg gtttttttct tctctgatct ttttgtttct atcattttat tggctaatat
     9781 gggcaggaat agcaggtgca ttagtacagg caagtctata ttactggttt ttgtggaagc
     9841 aacaagaaaa aggattggat tgggagaaaa tggtgcatgt agaagaacat aggttgcatc
     9901 agatctatca atttatcaat ttattcacag atgttcctga aattacggca aagatcaaaa
     9961 gaagacgcta tttggatcct atattgactc atattaaaag agaatcaaaa aacacttatc
    10021 tttacttgtt tgcaagaaga ttggcaagag gttctgattt tagtggtctt tatttccgat
    10081 tagtaattat aggtgggtta ttgattgccg gagtagtcga cttttatttt agtctgatta
    10141 tcggtttctt atttttatat ttgatcggtt tccaactgtt acctttagcg aaccagttcc
    10201 gctatatggt tctcgttcag ctttatccaa tccataagaa tcagaagaag caggcgattc
    10261 agtatctact cacatgggta ttaattatcg cgtcggttat ttttggtttg attgctgcag
    10321 tcgtattaga tgggcagcag cgatgggttc cgctagcggc ttatttgatt gttactatgc
    10381 tctttgttaa actatatgta ccaactcgtt tgaagaagat gtcagactga aaacaaagct
    10441 gtgtagggta aaaatgagaa tgaaaaatag agcgttgaca gtaagaatgc tgttcgttct
    10501 attttttttg agaaaagctg gttttttgaa tggcaattaa atgaaaaaga acgtatatgt
    10561 gcttgacgtg tagtgttcag gcgactagaa taataagtac tgcaaaacaa agagtaagag
    10621 gacgggagaa tgcatgatgg agtttcagtt agaccaagag tggcggttac gtcccatcaa
    10681 aggcgacaca ggaaaaacat acataggcat gcgtgatgag gacaaagtct ttattaaacg
    10741 aaacacgact ccgatgttgg ctgctctttc aagggaagga attacaccaa aacttgtttg
    10801 gacaaaacga acggggaatg gcgatacttt gacagcacaa gagtggctgg aaggtcgctt
    10861 gctgactcct aatgagattg gtcaaagaaa cgacgtgatt gatgttttgt accatttaca
    10921 tcattcaaat tctcttaaaa ccatgctgac ccgtatcggt ggtcgtgtca tgacgccgga
    10981 gaagatgctg caaaaatatg aaaaagaatt accggaagaa ctccagaaaa atcaatttct
    11041 gcaaattgtc catcgttttc ttcgtgagca gatcccgtca atagataagg aaaacttatc
    11101 agtagtacac ggagatgtaa accatcgtaa ttggttagta tgtaaaaatt atttatatct
    11161 agtcgattgg gattcaatca tgtttgcaga ccctgcggtg gatattgcaa cgattttagg
    11221 aaattatgtg ccactgtcta gctggaatca gtggctgatc agttatggca tacgcccgac
    11281 tgacgaaatg cttgaaaaat ttcattggta tgcggccatg aatttgctcc aagaaatcac
    11341 cagatattgc aaacgtgaag atcatcgacg gatgaacgaa gaaattttac aattaaaacg
    11401 aattttcagt ggctgaggtt tggcccaaac tctgcggaga tttggccaga cctttttaat
    11461 tagaggaaag gaatggagat aaataatgcg tgtccgaaat cgtccgggag cagcggaaat
    11521 gttagcggct cacccgaatt ttgtaattag tgatccaaca ttgtggaagg gaaaatggaa
    11581 tgaattgttt gaaaacgatc atccaatcca cattgagatt ggtatgggga aaggccagtt
    11641 catcactgga atggcaaaag cccatcctga aatcaattat attggagtag aaatgcaggt
    11701 cagtgttgtt tcgattgcac tagacaagtt gatcgaacaa ccgcttccta acctgaaact
    11761 tctacatgtg gatggatcag cgttgacaga gtatttcgct gacagcgaag tagatcaaat
    11821 ctatctgaat ttttccgatc catggccaaa aaaacgtcat gaaaaacggc gattgacata
    11881 taagactttt cttgctgtag atgaacagat tcttcgtcca aatggagaaa tccatttcaa
    11941 aacagataat caaggattgt ttgagtattc tctggcaagt ttttctcaat atggcatgat
    12001 cctcaaacag gtatggttag atttacatca aagtcaattt gaaggcaata ttatgacgga
    12061 gtatgaagaa aaattttctt caaaaggtca acgtatttat cgagtagaag ctcaatttca
    12121 agataagacc aaaaagaaaa gtctgtgaaa ctaactttct gctttcataa aaacattaat
    12181 atccataaat aaatcccaga aaatgaacat tacagatgta atgtattttc tgggatttat
    12241 ttgatttaaa atgtatttat ttcatcggtt tttccacaag gtggaatcaa ttggaagcga
    12301 gaaaatgaaa ataatccttt gtcggtgatc ttcaattcgg gtatgacaga taatgttaag
    12361 aaagaaagtg tcaaaaaagg atcaaaggtt ttagctgctc ctagttgatg tgctttagtt
    12421 attaattcat tcaactgttc atagacgatc gaaaaaggtt cctgtgtcat gattccgcct
    12481 ataggaagag aaacacaagc tatttcctgc tgtttgttga caacggtcat cccgccacct
    12541 ttttgaatca aaagattagc agcatacagc atatcctcgt cgtttgtccc aacgacaact
    12601 agattatgcg agtcatgggc aatcgttgtc gctatcgctc cttgtttcag ctgaaatcct
    12661 ttcactatgc ctaatccaat gtttcccgtt tgatggtgac gctcgatcac cgcaatcttt
    12721 aattggtctt tttcgattga aggaatgaat cgttcgcctt gtcggtccac tttttctacc
    12781 aaatctttag tcactagact attcgggata atttcgatca catgggcttg tgaagaagaa
    12841 agaagcagct ctaacgaatg tggatttaat ggttgagcgt ttagttttgg cagacaacac
    12901 gtatatgaat ttgaatgatc agcaaataaa tggtcttgga gttgcccaga ttgaacaact
    12961 aatgttccct tatagaaaac ttcatgaatt ggcagcttgg taagatcgtc tgttaagaag
    13021 aaatcagctt gatacccagg ggcaatcgca cctagtgttt tcaaatcaaa acattctgct
    13081 gcattcaatg ttgccatctg aatggcttgt aatggatcga ttccaagtgc aatagcttga
    13141 cggacgatat gatcgattga tccttctttt actaaatcat tgatcagttt atcatcggtg
    13201 acaaagaagc atcttcttga attttcaggt gtgatcgccg gtagtagcga tgccagatct
    13261 tttgcaacgg tcccttctcg aaccattaaa tacataccaa gatccaaacg ttctttagct
    13321 tcttttgcag ttgtagcttc atgatccgtc cgaatacctg ctgcaggata aacatttaac
    13381 tcttccatgc cgataccagc tgcatgcccg tcaatttttt tcttatgctg gtgcatcaat
    13441 tggatctttt ctaggatgtc tgtttcatta tttgctactg cttgatagtt catgacttca
    13501 gctaagccta aaacttcggg tttttgtaaa aaaggggcta atttttttgc atttaaattt
    13561 gcaccattgg tctcaaaagg ggttacaggt acacaagaag gtagcataaa aaagatattc
    13621 atgagtgttt gtcttgcatc ttctatcata aactcaagac ctgtactccc agcaatgttt
    13681 cctatttcat ggggatctgt cactacagta gtaactccat gaagcaagga tacttttgaa
    13741 aattcgcttg gtgataaaag tgagctttcg atatgcacat ggccatcgat gaatcctggc
    13801 actagatatt gcccattagc attatgtacg ctttttcctt cgtattcacc gattcctacg
    13861 atcattcctt cgcagattgc tacatctttt tccatgattt ctttcgtaaa gacgttgacg
    13921 atcttagcat gcttgatcac taggtcagca ggcttttttt tacttgctac ttcgatatat
    13981 ttccttaatg ttgtttccaa tggagtgcct cccaagataa atttttataa gtataacata
    14041 aattcggttt ttgccaatat ttttttgtaa attcatttta tttgaaacaa aaagatcaaa
    14101 aagacgtatg ttttttgtgg aggggagaat aatggaaata aaacgattat cggatcaaat
    14161 gataagtgaa agttttgcca aacatgccca agacgtatat atattgccag cttcagaagt
    14221 attttacatc tattttcgct atggaaaaaa acgaacattc tggaagaaag tctcttcggc
    14281 agatggacag caactgatct caagatttaa gtatttggga cagatggata tcggagaaaa
    14341 aagaaaagct cagttaggtg catttactta ttctttagaa aaacaggaag ttcgtctgcg
    14401 gttgtcttct gtaggagatt accttcaacg agagagttta gtgattcggc tattatacgg
    14461 tacttttgaa acaccccatc gttgctttag ttctcgagat tttgcaacta ttttacagaa
    14521 aaccgtcaga cgtggattat accttttcag cggaccagtc ggatcaggca agacttctct
    14581 aatgtatcgg ttggctttgg aagaaaaatt gcaagtgatt actatcgaag atcctgtaga
    14641 gatagaagaa ccgttttttc tgcagctaca gatcaatccg aaaatagcac agacttatga
    14701 tacacttttg agactcgcat tacggcatcg tccagacttg ctaatcattg gcgagataag
    14761 agatgaatca actgcacatg ctgcggtaag agcagcacta acagggcacc gggtttttgc
    14821 aaccgtacat gcacgcaatc ttgaaggtac attggtcaga atccatgaac tgacaaagaa
    14881 aaaagaagag ctatctgaat gtttagctgg tgtcatttat caagaacttt tggaagatgc
    14941 caatcaccat ccttctgttt tatggagtta tcaatttatg gaggaagaat cagtaaaaaa
    15001 atcatgggag gaaagttatg aagaagctca ggaaaatatt tggcagtcag aactattcta
    15061 aaggattaac aagaagccag caaaaccagt ttgcacaaac catgggagaa ctgcttttat
    15121 ctggttttag tctgcaagaa tccctttatt tattactcac gatccgttcg tttccacctg
    15181 ttattttggc aaatatccaa catcagctaa aggaagggca agcgtttagt gaggtacttt
    15241 cgcaaatagg atatggagca gagcaactgg tacagatcga gttagctgaa cgtcatggga
    15301 atctgccgac aactttgttg gaaattgcca aacagtttcg gcttgttgaa ggatttcgca
    15361 atgatttgaa aaagctgctt gcatatccga tgctattgct cacctttttg ctaggtatct
    15421 tgtttagtat gcggttgttc gttttgcctc agcttattgg ttcaggaatg gtcatgcaag
    15481 agcattgggg tattcagctg atacaagtct ttcattggta tgttctacta ttcatcctag
    15541 cggttactct attggcttta ctagtaaggt atcgtctgat gaagttgtct cctgttgatc
    15601 agagtgaatg gataagcaga caattattga ttggaccgat atattccttg tatcaatcgg
    15661 cttatttgtc actagagatt ggaaagcttt ttcatgaagg tttcgaatta aggcaaatca
    15721 tagtctgctt aaaggaaacg aaacaagaaa gtctgattca gcatcttgcc tctaagttga
    15781 ttcaaggatt ggaggcaggt atgtcattgg ctgagcagtt ccagaattat cgttttctat
    15841 cagaagaatt cagtaaaatt gttttacagg gagaggcaaa gggaagttta ggtaaagaac
    15901 tgatttttta cagcgaatgg acaagaaaac agcttttcaa acgtcttcat cactggatgc
    15961 attttattca gccaatcatt tttttgggcg tagcggcttt gattttatta gtttatgccg
    16021 ctgttttatt acctgtttac ggaagcatag aggaggtttt gccatgaaaa agagaaaaat
    16081 atcttattat agcggtttta cattgatcga aatgctaatc gtcttactga tcatcagtgt
    16141 attagtgttg ttgttcgtcc ctaatttgtc gcgttatagg aatcatgtag accaagaaag
    16201 tcgtgaagcg atcatccagt tggtagacac acaaaaagag ctctatgcgc ttcaaaataa
    16261 tggaagagtt cctactgtgg aggaattgtt gaatgaaggc tatatcaaac gagaacatgc
    16321 agaaatctat cagcggcctt gaaggtaagg ttttagcatc aggatatacg ttgtttgaat
    16381 cactcttact gattttatta cttacaacga tcctttcttt tccagttctc gcattttctg
    16441 catggaagaa tgaattggca gtctatcaat ttttcggcca atttgaaaaa cgaatctatt
    16501 ctacgcaaaa aatcgctatt gtgaatcaga tccaaacagg cttttattgg aatagtgaag
    16561 agaatcaaat catttttcat gttccgaatc cagataaaat aaattgggaa gtattagata
    16621 ttcctgatga gattaccttg aaacgccatg cttctattac ttttgctgct agaaccggga
    16681 atgaaagttc actaaaagct tatcaatttt attgggaaga aaaagagcgg acgattacgt
    16741 atcaattcca gatagggagt ggtcgctata caaaaagaat tgaataaaac aggatttgtt
    16801 ttgttggagt cccttacagc gcttatgatc gcttctgtct gtgtctactt agtgagcttt
    16861 ggccagctgt ttttgcttca ggcagaaaag aaaagtgggg aggaactgct atttattcga
    16921 atgatttatg aagatatcaa gacgcagcgg atgtatcaca aaagaccaga gcgaagggaa
    16981 gaaatgatcc catacgatat ccaaattgtc gataataatg gagtaatcga aaaagtgttt
    17041 gtcaatatgg agaaagaagc gatcagtatt gaaaaaacaa gtaattccag catttacgct
    17101 gattgaatgt ctaattggac tgatgatatt gagcagtcta ttgctcacat tcaatctttt
    17161 gattcagcaa accatccaag taagtgaatt tttgcagcgt aaagatcaaa aagagtggct
    17221 aatattttta gcacaattag aagaagaact caagagcagt cacaatgtct ctgtgaaaaa
    17281 tcagcaactg cactacacga tcgaggataa acagtatatc atcgaaagat atcaggcaat
    17341 gatacgtaaa agaagaactt ccggcggaca tcaacctatg ttaacaagta tcactgagct
    17401 tcaattattg gaaaaagaac acacaatttc tttttatgta cactttgaaa atggagaaga
    17461 tggatatgcc atatggacaa aaaacgatca gtagaaaatg ttataagggc tctattttat
    17521 tttctgtctt gcttttattt cttttatgta cgtttttatt gatggcagca ttagaaagct
    17581 accgtttgtc aatggatctt aatatacgaa tcaaaaatta ttatatagca aaaataatga
    17641 agcagatggc tttatcggaa cagtctacgc ttgccgaaag ttcggaagga gttttttact
    17701 acacaactgg atctgtaaca tatcagtggg tacaacagtc cctattctta gaggtggaag
    17761 tctcaccgtt tatctttcgg ttcacagaag aagtaaaaaa tgatacagat actagtacag
    17821 aatgatggaa tgttcgtgaa aacgccctct tttctgttat aacactgttt ttaataaaaa
    17881 aatgtttgcc gtttcacaaa taaaaaacag tgaaaacgat tgaaattttg tcaattgttc
    17941 gatagaatta aacaggtgaa aaaattgtta taatatatct agaaatgagg tgcaccatgt
    18001 taccagaaaa aatggaacaa gggtttaatc aaaacctgga agctattcag cttttacaga
    18061 atgcattggg aacttccttt ttagaagcct atgtcgaaaa tgcagaaaat ttaatagatg
    18121 attatcaagt acgtgtggtg gatggagtgc cgacaaaaga gacaacgcaa cggatcacag
    18181 ctctttatga agagttgaag aaactttctt tcgagccaga agaatggcgc agactgtctc
    18241 aattactttt attgaagggc agccagacag aacatttgca ggcgaaccat caactcactc
    18301 ctgatagtat cggtttttta tttgtttttc ttattgaaca actatacaca aataaaaaag
    18361 caccggttaa gattctggat attgcggcag gaatgggcaa cttgttatta acagttcttt
    18421 taaacctatc gaatgcgggg taccaaacag aaggaattgg tgtggatatt gatgatacat
    18481 tgttggctgt cgcagcttct acaagtgatt tgacacaagc aaatgttcaa tactttcatc
    18541 aagacgggtt gcaggaatta ttaatagatc ctgtcgattt tgcaatcagc gatttgccaa
    18601 tcggttatta tccgaatgac gaaaaagcaa aagagttttt aacaagtacc gaagaaggcc
    18661 atagttatgc acatcattta ttgctagagc aatcaatgaa atatgtgaag ccggatggtt
    18721 ttggtttgtt tttgatgccg agtggatttt tagaaacgga tcaaagtgaa gaaatcaaaa
    18781 aatggtttaa agaagaaggt tatcttcaag gaatgattca attgccagat gaattgttcc
    18841 gtaataaaca atcgcaaaaa agtattttga tcttacagaa aaaaggacca caagcacaac
    18901 aagtaaaaga agtcttgctt gtgaaacttg cttcattaaa agaaccagag aaggtgacgg
    18961 aatttttcaa cgagtttaaa aactggaaat catctgctct gtaatcattc aacgagaagg
    19021 agagtcatat gtctaaaaca attgcaatca atgctgggag ttcaagtttg aaatggcaat
    19081 tatatcaaat gccagaagaa accgtagttg cgaaagggat cgtcgaacgt atcggtttac
    19141 aagattctat ttttaccatc aagtacggcg ataatcaaaa attcgaacaa atcatggata
    19201 tcgaaaacca cgaagtagca gtaaaaatgc tgttagatca gttgattgaa ctgcaaattt
    19261 taagctctta tgatgaaatc accggagtag ggcaccgtgt tgttcacgga ggagaaactt
    19321 atggggattc agtagtgatc gatgagaaag taatggaccg cattgctgaa ttagcagaat
    19381 ttgctccttt gcataatcct gcaaacttaa tggggatcaa agcgtttaaa aagattttac
    19441 ctgacatttt gagtgtggct gtattcgata catctttcca ctcaacaatg ccaaagcaaa
    19501 attatttata tagcttaccg gtgtcttatt atgaagattt caaagtaaga aaatatggct
    19561 tccatggcac aagtcatcgt tatgtgtcgg aacgtgcggc aaaattattg aacaaaccga
    19621 tcgaagaact aaaaatcatc acttgccact taggaaatgg tgtatcgatc acagcagtcg
    19681 atggaggtaa atccatggat acttctatgg gattcacacc attagctggg gtgacaatgg
    19741 gtacacgttc aggtgatatc gatccagctg ttctcccata tttaatggaa aaattagaga
    19801 ttgatattga tgaaatgatc aatgttttga acaagaaatc tggtctgcta ggcctaacag
    19861 gattgtctag tgatatgcgt gatcttgaaa agaactatga aaaagaacat attcgtttag
    19921 cttatgacat tttcgtggac cgcatccgaa aatacatcgg cggttatgtg acaacgatga
    19981 atggtgttga tgcaattgtc tttactgctg gtatcggcga gaatgacgga catgtacgta
    20041 gtgaaatcat caaaggtatg tcttggtttg gttgtgaaat cgatccagcg ttgaatgaat
    20101 tgcgtggtga agaagtagat atctctacaa aagaatcaaa agtaagagta ttagtgatcc
    20161 caacagatga ggaactaatg attgcccgtg atgtggagcg tctgcgcggc taagccaaag
    20221 cattcgttag gataaaagat gaaacgcatg caagagggaa ttcttgcatg tgtttttttt
    20281 gcggagaaga ccgcaaaatg gtaatttaac ttcaatttcc cttctttttt acaggatctt
    20341 cttagggaat tttgcttgga aaagtggtca aaagggaaaa gactggcatt ttatttattg
    20401 attttcagga aaatcccaat attttatttt cattcatctt tataagggac attacatatg
    20461 taaaaagaaa aaacattttt agatcagcct cgtgggctgt cgactttatt cttcgcttta
    20521 tgtttagcta gaaaaccgat ccgaaacatt cattgatcga ataataaaaa cttgattttc
    20581 cacctgtact ttgcctgaat cgtgaaagca caggtatgaa aatcaagttt tttgttgcga
    20641 aaaaacgtcc tagtcaatca gttggccgag cggctggcgg ctgatctgct cctgatcaat
    20701 aacgagtgct tcttggaagt gttgctgcaa aaagtagcgg tcatggctga ctgctaggag
    20761 cgttccttga tagtcttgca gcatttcttc aatttcttct cgagcatagc tgtcgaggtg
    20821 attggttggt tcatccaaaa tcaggacgtt gattttttct aaaaagagtt tcagcaacgc
    20881 caaacggatt ttttcaccac cggataaatc tttgatccgt ttacccacat cgctttgata
    20941 aaagccactt ttggctaatt gccgtcgagc gacttcttcg tttccagtca atgtgatggc
    21001 gtaagccagc aaacggctat cgggatcggc aaactgcagc tgctgcggca aataaccgat
    21061 ttgcaagcta ctacctaatt tgatggtgcc accgtaaggc ttttcctcac cgagtaataa
    21121 tttgagtaag gttgattttc ctgcaccatt gtcgccgatg atggctaaat gatctccacg
    21181 atagactgca aaactgctgt ctttgaatag tacttgatca gcgtactgct ggtgcaagtt
    21241 ttgaatgatg aaaacttctt tgccagattt tccaccatta gacaagcttt gcagccgatt
    21301 tttcggggga ggtggcggtt tgacgagtgt cagtttcgcc aggcggcgtt ccagttcttt
    21361 ggcttttttg aagaaggact cattgtctcc ttcatgtgcc cactgacgaa aacgacgaat
    21421 catgactttc aaccgctgga cttctttttg ctgcagttcg taattttttg tcagctcagc
    21481 caagcgagct tctttcaaga ctcgaaaacg gctgtagttt ccgggatact caatcaatgc
    21541 accttcttcc agttcccatg tttttgtggt gacttgatcg agaaattgcc gatcatgaga
    21601 aacgaccaga accgattttt ttgtattctg taaatagttt tccaaccagc gaatactgtt
    21661 aagatctaaa tgattggtcg gttcatcgag taaaagtaaa tcggcatcgg ctgctaaaat
    21721 tttggctaaa gcgactttaa ctcgttcgcc tccgctaagt tctgttaacc gtgcttcctg
    21781 acggtgtgga atccctaagc cttgcaagat cgctgcaatc cggtcttcta atacgtaacc
    21841 gcctagttct tcaaagcgat gctgcagctc accgtaacgg gccagacact tctctaagtt
    21901 tgtatctggt tctgtcatca atgcttcgta gcgactcatt tgctgtttga ggtcctgtat
    21961 ctcagcggca gaatggagca agtagtggtt gacggtcgct tcggaaactg taagttcttg
    22021 ttcgaccgtc ccgatcttta agcctttttt gcgactgata acaccgctgt cgacaccttc
    22081 tctgccggtc aagatttgta aaaaggttga tttgccagtt ccgttgatac cgactaaggc
    22141 aattttttca ccagcttcaa tttgacagga taaatcctca aataatggat tcccgccgat
    22201 tgttttttta atagaaattg cttgtatgat catgatctct ctccttgaga ccactcaaaa
    22261 aggccatggt tatccatggc ctgtgttatt taaactcaga ttatcatgag aaaaatacct
    22321 tgagatggcc attgatttta cgtgaattca actgttttgg acaaacttgt cccgtcaaca
    22381 gcaactgtaa aaccaattgc atggctaaat gcgatatggt atcgttggta attttcatta
    22441 cttgttattt tagtcatgcg catcatagta ttttccctcc ttcgtgtagt tgtcttttcc
    22501 agtgtacctg aaagtgtcag aaagttcaac tagcgctgac atttacttaa tatttatcga
    22561 tgtgtaattc tgtcagagag ctttttttat ctgactgagg gtaatgtctc gtggaccaca
    22621 tcattgtatt ctgagctaaa ccagcgttca ggcgaaatca tatggtgttt gataaaaaac
    22681 tttgtctaca tgacttcctt ttgccatttg cttaataccg ttgcattaaa cagctcttct
    22741 ctttggaaat ctcaccaaca tcgcgttcag gagtatcgtt gtggttgcaa tggcaagcgg
    22801 tagcctttca tcaattcttg aatgatttac cgaatgtagc acttgccttc tcgcggtacc
    22861 cgaacccgaa ataaaatttt agtttggcga taaaatcggt atccgccatc gatacttcat
    22921 attttttcgt atcagattca tcggaaactt ccacattgac gaaccaataa gctttggcat
    22981 gcttaggtcg ttttaaacca caccagggcg actaatcgcc ctggtgttca ctttttttta
    23041 ttttacttga actcattgac tgttttccct ttattttaaa cattataaag accgatgagt
    23101 gcttgccgcg tcttttgttt ttatcacggt tttttgtgca aaaaaacctg ccgatagttg
    23161 gcaggtcgtc tacgtgttaa gctttggtgt ggtctttagc tggcaattgg ttttctaaat
    23221 cagttcggac aacaagcaca tcgcaagctg cgtttctaat aacatattca gaaacagaac
    23281 caataaataa acgttctaca gcattcaatc ctgtggctcc taacataatc aaatctactt
    23341 gattatcttc tgggatttct ctggcaataa tttgttttgg tgagccatat tctacgacag
    23401 aggttacatt attcaagcca gctttcttcg cattgctttc ataatctgcc aatgtttgct
    23461 ttgccatttc agtagcttgt tctgcaagca ttccatcaaa ggatgaaacg gtttggaaag
    23521 aacgtgtgtc aatgacatgt gctaatagaa gttctccgtt atttcggata gctacattga
    23581 ccgccttttt aaaagctaat tcagcttcat cagaaccatc tacggcaacc atgatttttt
    23641 tgtattgttg taacataagc attcactcct caagtcaatc gtattgtact cttttattgt
    23701 agaacacttt agggaaaaag agaattattt actgctttgg ttcagcaatt ttttttgcaa
    23761 atcggatact gaaggtggca gaaacaataa ttacgatcag taaaaagacc aaggcaataa
    23821 agcgttgatt gaaaaaagct gttaaaatac ccattacgcc taaaacagca tatgcagctc
    23881 cgtaaaattg taggaatcct tgatttttat catttttgtt gatcaataca aaaaagatat
    23941 cctgtttatt aaataaataa tagctaacga ataataatac taaagtggcg ataatcatta
    24001 aaatcctaat catgaacaaa tcctccaaaa taagcaacaa atagaaaaag caatttgcca
    24061 agagcaaatt gctttattct ttaaaagatg gtaatctccg ttaatgccgt tgtttgtcct
    24121 gtagtattga tcccgcgaat tcaagcaggt gggtcccatg gtcgtagctt cgaactacca
    24181 aatgataagg catgttacca gcagtgacac aagacgggtc ccaagatatc aataaaaatg
    24241 ttcggtcaac acacaaagag gacaattcga acatcacaga tttcccacct ctatagtagc
    24301 atatgaattt aaaaagagca atgagagagc tcgcagaaac agaaccgttt tcttgtctag
    24361 ttttttggag gatgcttttt ccactctttt atacggtaat actgttgacc cagagcctgt
    24421 tcatatttcc ctgtatcttt tggctgataa tactgagcat tcttcagttt atctggtaaa
    24481 tattgctgat tgacccaagc ctgatcaaaa tgatgaggat actgataacc cacgccacgg
    24541 ttgagtgatt tcgcaccttt gtaatggcta tctctcaaat gatcaggaac atctccagct
    24601 ttgccctctc gaatatcagc aagtgctgca tctaatgcca tgtaggcaga attggatttt
    24661 ggcgacaagc aaagatcgac gacgacgtct gctagtggga tacgtgcctc aggcaatccc
    24721 aatttttcag cagctaatac agcgttcact gtacgagccg cagcagcagg attacctaaa
    24781 cctatatctt cataaccgat aaccatcaga cgccggcaaa tacttgccag atcacctgcc
    24841 tcaactaaac gggcgagata atgaagtgca gcatccacat cacttccacg aatcgatttt
    24901 tgaaaagccg aaataacgtc ataatgagca tctccatttt tgtcatgggt aagagctttt
    24961 ctttggacac attcttcaat gatcgacagt gtaagatgaa tccttccttc tttatctgaa
    25021 ggagtcgata gtgtagccag ttccagccca ttcaatgcac tccgcaaatc gccgttcgtt
    25081 gcacgcgaaa ggtgaagaag agcatcttcg tctaactgga ttgcttgttg tccaaggccg
    25141 cgttctttat cccttaaagc atgttcgact gccaactgga tgtcttgttc tgttaatggt
    25201 ttgacttcaa agatctgtgt acggctgcgg atcgctggat tgatggtaat ataaggattc
    25261 tcagtagtcg ctccgatcaa gatgattcgg ccactttcta aatgaggaag cagaaaatcc
    25321 tgttttgttt tatctaatcg gtgtacttca tccaataaga ggatcactgt accactcatt
    25381 tttgcttctt ccgctacaac ttgtagatcc ttttttgaat ctgtagcggc gtttagcatg
    25441 cgaaatgcat aattagtaga gccggcgatc gcacttgcga tactcgtttt tccagtacca
    25501 ggaggtccat atagaatcat ggaagagagc atgcgagcat cgaccatgcg gcgtatgatt
    25561 tttccttccc cgactagatg ctgctgacca acgacttcat cgatcgttcg aggtctcatg
    25621 cgataagcca atggctgttg cataggattt tcctccttct tgtttttacg tcccttaatt
    25681 atatcatatt tagcaagatg cctagaaaga acagccttct gttgttttgt tttcatagct
    25741 tatccaacta atattgtgta tgataaagta ggtgagaaaa atgacggata aacaaaagat
    25801 agatgaattt ttttggaata aaacgacgcc agaagtggca cgggatttat tggggatgta
    25861 tttggagcat gacacaccag aaggaagatt agctggatat attgtagacg cggaagcata
    25921 cctaggtcca gaagacgagg ccgcccatag ctatggttta agaagaacac cgcgtgtgcg
    25981 ggcaatgtat gaaaaaccag gtacgatcta cctttacacg atgcatactc atcggatttt
    26041 gaacatcatt actcagccag aagggattcc tcaaggagta atgatacgtg caatcgaacc
    26101 cgccgcaatg attgatcaaa tgagtaaaaa tcgtggcgga aaaactggtc cagatatcag
    26161 caacggtccg ggaaaattag tggaagcatt agcaattcct caagaacttt atggtcagtc
    26221 tatagcagat agttcgttac gcttagtttt tgagaaaaaa aagacaccaa aaaagatcaa
    26281 tgctttgcct agaatcggta tcccaaacaa aggtgtatgg acagaaaagc cgttgcgttt
    26341 tgttgtgtcg ggaaatcctt atatctcttt acaaagaaaa aaccaaatcg agaaagattg
    26401 gggttggagg aaagaaaatg aaaaagaagg atctattaac atatttagat gaaataattg
    26461 aaaaacaagt gacagactat gatgtagctt tagattggga tacaaaaaat catacgatcg
    26521 agatcgttgt tcgcttgttt gcagaaaata aagcacaatt tgagattgat gatgcagaag
    26581 ggatcgtttc gcaagaacca attatcgaat ttgaagacgg cgtacttctt tttaatcctc
    26641 agaaatctgt ttttgatgaa cgcaattatc tagcagtaat cccttatgaa ggaaaaaaag
    26701 ggttggcaaa atcagtagct gattctttag tggaatatct aaatgaagta ttagctcaag
    26761 ggcaaagcga tttgcttgat tttttggatg aagacgatga cgaagctgta ttcgagcttc
    26821 actgggataa tcagcaacta gcagaattag tagaagtaaa acagcaaaat gaagcagata
    26881 cttatttacc gtacccaagt tattaaggag tgacagatat gaaatggaat gaagtaaaag
    26941 tagaaactga aagtgaagca gtcgaagcag ttgctaatat tttgatggag gcaggggcga
    27001 gtggtgtcgc gattgaagat tctttagatg ttgaacattt tcagtcggat caatatggtg
    27061 agattttaga taaaaatgat tttacatcgc tgaaagaagg cgcactagtc atggcttatt
    27121 ttccggaaac gactttctta ccggaaatct tgccttttat caaagatcgt atcacacaat
    27181 tacctgagtt tggtctagct attggaaaaa ataaagtaac agttagtgaa gtagaggaaa
    27241 gcaactgggc aacagcctgg aagaaatact atcatccggt gcgtattacg cgaatgctga
    27301 cggttgcgcc aagctgggaa aactaccaga cagaagatcc tctagaaaaa attatcacac
    27361 tagatcctgg tatggcattt ggcacaggca ctcatcccac gacttccttg acattgacag
    27421 cactagaaac agtcttgcgt ggtggtgaaa cattgctaga tgtgggaact gggtcgggcg
    27481 tgctaagtat tgcagcaaaa tatcttggtg caaaagatgt ctatgccttt gatttagacg
    27541 aagtagccgt tcgttcggcg aaagaaaata tggatatgaa tgaaatagcc aaagatgtcc
    27601 atgtatcagc taacgatctt ttgacaggaa tcgagattga aagtgatgta atcgttgcga
    27661 acattttagc agatatcatc ttattgatga tcccagatgc ttggcgttta ctgaagcaaa
    27721 caggcacgct gatcgtttca ggaatcattg aagagaagaa gcaaatggtt ctggatgcca
    27781 tgttagaaca agggtttatc gtggatcaaa ttttccagca aaaagactgg tatgcaatta
    27841 ttttgaaaaa accagaggtg gaatagatgc agcggtattt tttaaaagaa tcatatgaat
    27901 caaaagacta ttttgtttta gaaggggaaa actatcacca cattgtacgg gttatgagga
    27961 tggaacccga ggatgaagtt tttttggcat tcaatgatca aatagccatt gttgcagaaa
    28021 tcacggagat tactgaagag gctgtttatc tgaaagaaat taaaaaagaa gaaaatcaaa
    28081 aggaactgcc aagttcgatt accatagcga gtggctatcc taaaggggaa aagctggatt
    28141 ggatcgttca gaaatccact gaattaggag cgcaagcttt tcttggtttc ccggcatcag
    28201 cctctgtcgt taaatgggat gcaaaaaaac gtatgaaacg acaacaacgg cttgaaaaga
    28261 ttgctcaaga ggcagcggaa caatcccatc ggcagatcgt gccatcagta gagttattgg
    28321 aaacgaaaca agatttattt gagcggctag atgattatga cgtggtcttg gttgcttatg
    28381 aggaatcagc aaagcaaggc gaaaaatcaa atgcagccaa aattcttagt gaaacaaaac
    28441 cagatagtcg aatcttagct gtctttggac cagaaggtgg attatcccgc gatgaagtcg
    28501 attattttgt agaacacaaa gggatacttt gtggattagg accaagaatc cttcgtacag
    28561 aaacagcacc tctttattta ttaagtgctg ctagctatca atgggaatta atgaatcctt
    28621 ctttcagcta acgttcttca ttaataaaag agtatcgaca gaaagaattg aaaaaatagt
    28681 caacaatttg tataatagaa acaattattt atacaatgga tacgaaaaat cgtttgaaaa
    28741 agcttttaaa aagcgaacat ttccgtaaaa aaacgtataa ctgcacggat tttgggacaa
    28801 ttatcaaaca gatatttgtc ccgattcccg ttttaccgaa aaaagaaagg gggatcgatt
    28861 cctgtgacaa aagaagaaat tttaacggga cccggtgtca taaagctcgt tagtcaatac
    28921 atgggtcccg aacatgtagc atttgttgag aaagcatgcg aatacgcgac tgctgcacat
    28981 gacggacagt ttagaaaatc aggcgaaccc tacattattc atcctatcca agtcgcaggt
    29041 atattagcag atttaaaaat ggatccccat acagtggcta caggcttctt acatgatgtt
    29101 gttgaagata cagaaatcac tttagaagat ctgagagaag aatttggcga tgacgttgct
    29161 atgttagtgg acggcgtaac caaattaggg aaaataaaat ataaatccca cgaagagcag
    29221 ctagcagaaa accaccgaaa gatgctgcta gcaatggctc aagatttacg agttatcatg
    29281 gtcaaattag ctgatagact acataacatg cgtacgttga agcacctgcg agaagataag
    29341 caaagaagga tcgctcagga aactttagaa atctatgcgc ctcttgcaca tcgtctaggg
    29401 atcagccgga tcaaatggga attagaagat acagcacttc gttatctaaa tccaaaacag
    29461 tattaccgta tcgtccactt gatgcagacg aagagagaag aacgtgaaaa atacgtaagt
    29521 ggtactgttg aagatattcg aatagcgaca gaagagctgg ggatttttgc agaaatctat
    29581 ggacggccaa aacacattta ttcaatctat cgtaaaatga aagatcagaa aaagcagttc
    29641 aacgaaattt atgacttgct agctattcga gtgatcgtag attcaatcaa ggattgttat
    29701 gctgtattag gagcaatcca cacaaaatgg aaaccaatgc ccggcagatt taaggattat
    29761 attgccatgc caaaagctaa tatgtaccaa tctttgcata ctaccgtgat cggtccggca
    29821 ggaaatccgg tagaaattca aatccgaaca caggaaatgc atgaaatcgc tgaattcggg
    29881 gttgctgcac actgggccta taaagaagga aaaaatgaaa aagtagaacc agatggtatg
    29941 acgaaacaat taagctggtt ccatgagata ctcgaacttc aagacgaaag ctatgatgct
    30001 tctgaattta tggaaggcgt aaaaggagat atctttagtg ataaagtcta cgtcttcaca
    30061 ccaaaaggag acgttactga gttaccaaaa ggatccggac cattggactt tgcatacagt
    30121 atccatacag atatcggtaa caaaaccact ggtgcaaaag taaatggcaa aatggtgcag
    30181 cttgattaca aattgaaaaa cggagatatc attgagatca tgacttctcc aaattcattt
    30241 ggcccaagtc gcgactggtt gaaattagtt gctactagca aagcaagaaa taagatcaaa
    30301 cgtttcttca aagcccaaga tcgagaagaa aatgtgatca aaggccacga atccgtggtc
    30361 aaatgtatta cagatctagg atttacgcct aaagatattt tgacgaagaa caaactgcaa
    30421 gaagcactcg atcgttttaa ttatcaaaca gaagatgatc tctatgcagc tgtagggtat
    30481 ggagaagtta gccccttgac gatggccaat cgtctgactg aaaaagaacg taaagaacaa
    30541 aaaatcgagc agcaaaagca agaagcagaa gaaatcatga atcagccgaa aaaagaacct
    30601 gacaaaatga aagtacgtca tgaaggtggc gttgtcattc aaggggtaga aaacttacta
    30661 attcgtatca gtcgctgctg taatccgatt cctggtgatg atatcgttgg ttatatcact
    30721 aaaggcagag ggatatccat tcatcgtcga gattgtccga atgttcagcc tgacaaacca
    30781 aatgtagcag aacgtttgat tgaagtcgaa tgggaagata catcgaatac acgaaaagag
    30841 tatgatgcag atttggaaat ttacggctat aatcgttcag gcttattgaa tgatgtactt
    30901 caaacagtca atgcgctaac gaaaaatctc aacagcgttg aagcacggac gaataaagat
    30961 aaaatggcga cgatccattt gacggttggt atccagaatt tatcccacct aaagagtatc
    31021 gtggataaaa tcaaagcagt acctgatgtc tacagtgtac gccggacgaa tggataggag
    31081 gacagatatg agagcagttg ttcaacgggt aagtaaagct gctgtgtcag tcgatcagca
    31141 agaaattgga aaaattggct acggcctact tgttttatta ggcattcatg aaaccgatac
    31201 gcaaaaagat gtggactatc tggtaaaaaa aattgcacaa atgcgtattt ttgaagatga
    31261 acaaggaaaa atgaatatta gtgtagaaga tgtgggagga caaattttaa gtatctctca
    31321 attcacactg tttgcggata cgaaaaaagg aaatcgacca agctttgtgg aagccgcacg
    31381 accagaaaca gctattcctt tatacgaatc attcaatgaa ggaatcagaa gtcgcggcat
    31441 cacagtcgct accggacaat tcggagcaga tatgtcggtt tctctcgtca atgatggacc
    31501 tgtaacgatc attattgatt ctaaaaacaa ataaagtaat ctggttttca ctagtaaaaa
    31561 ctatgaaaca cgtcgcttaa gttagactga taaactctaa cttaagtggc ttttttgtct
    31621 gcaaaaagcg cttcttttgt cagcagtcgg aaaaaaagtt aagcttaaat tatacaagtt
    31681 attttttata ggaggttgct gaagatgaaa gaagtaccag tagtagatat caaaatcaca
    31741 gatttaaaaa aattgtatca aaaaggatac aacatgttgg aggagttacg ccatgaagcg
    31801 gacgctcccg tcgtcaaagc aaagattttc aataaagaag ctatcacgat ctacggatca
    31861 tcagcagcaa aagtatttta tgatccaagg aatttcaaac gaaaaggtgc tatgccaaaa
    31921 ttagtattga agacactttt tggtcaaggt ggagtacaga ctttggatgg agctgctcat
    31981 caccatcgga aaaatatttt tatggatcta atgacaccgg aaagaatgga agattatcat
    32041 cgtatcttag ataagaatct gacacaagca cttgaagcac agcatggtca atttgaacta
    32101 tttgatttaa gtaagatggt tttctttaca tctatttgcg agtgggcagg tatcaattta
    32161 tcagcgattt ctaaagatga ggtcgaaaaa ttagcagaat atcaaatctc tatgatcagc
    32221 ggaacgttta cttctccgat tgatcacata aaaggtgtcg aaaatcgcaa aaaatcagaa
    32281 aaatgggcgc aaggcttgat tgaagaagct cgacaaaatc ctgtggctgg aaaagaaaat
    32341 gtggcattat atgcttttgc caatgccaca gatttagatg gtcagctttt acctctggaa
    32401 gtggcagctg ttgaattatt gaacatcatc cgtccaacgg ttgctctaac tgtgtgggcg
    32461 gcattgatgg gacatgcact tttcagtaga ccagatttgt atcagcaatt aaaaaatgac
    32521 ttttcgacgt tacaagatcc gtttattcaa gaaatgagac gctattatcc tttcttccca
    32581 atgctacctg cgatttcgct gaaagaagta gaagtagatg gttatcgtat accagaaggc
    32641 agttgggtta ttttagattt atatggaaca gaccatgatg aacgaacagt tgaagcgcca
    32701 gattccttta tgatcaaacg gtatgtaggg aaagcaaaag atatttccta taaagaagaa
    32761 tatgagatga ttgcacaagg cgggggaaat tttcgacaaa tgcatcgctg tgcaggagaa
    32821 tggatcactc ttcatagtct acgtgtgttt tccgatcagc tagtaaataa gtttgagttc
    32881 tctgtacctg aacaagattg gacgattcca ttcaatcagt ttccgactta tccgaatagc
    32941 cgagcgctac tttacaagaa ttagattagc ataaacttat cgaaaaaaag gaagatcaaa
    33001 cagtgctgat cttccttttt tcatattact ataaattgat aaggtcaatt gatcaaaatc
    33061 cacgaaatgc ttcatcaatg gtttgataat cttcattgga taaatgcaaa tctaaagctt
    33121 ggacattgtc agatacttgc tctggacgct tagctccagg tataactaca gacactcgtg
    33181 gattcttcat gtaccaagct aaaacaagtt gcgcaggagt ggcttggtat cttttagcaa
    33241 tggggcgaag tacatcgaca gcggttaaaa tttctctgaa acgttctcct tggaaatctg
    33301 gatttctttt tcgaggatct ccttcaccaa attgtttttc ctcaccaagt tcatattttc
    33361 ctgtcagcaa accggaagca agaggaaaat agggaacaaa actgattttg tttttttcaa
    33421 gataagggaa taattctttt tcagcctgcc gatggatcaa actgtattta tcttctacta
    33481 catcgacata tccatctgca ttcgcttctt tcaattgttc taacgtgaag tttgacacac
    33541 cgactgctcg gatttttcct gcttctttta attcgtgcaa tgtagcaact gattcatttt
    33601 ttggtgtgct ttcatccgga aagtggatat aaaaaatgtc gatgtaatca gtttgcagac
    33661 gttttagtgc atcttccacc gattgtttga gaaactcagg agaattatca aatgttcgcc
    33721 ctttatttgg tacatgagca gcttttgtag cgatgacgac acgtgagcga tcatattctt
    33781 tttccttcag tacctcgcca atcagttctt cggaacggcc gtttccgtaa gcataagcgg
    33841 tatcgatcaa attgatcccg ctattcaaag ctgtacgaac tacttgtttt cctgtctcat
    33901 cttccaaacc gtcaaaaaga ttatgtcctc caacggcatt tgctcctaat ccaagctgtt
    33961 ctgcgtagac ttgtgaatgt ccgattctta tttctgccat agctgattcc tcctttttgt
    34021 ctagtctaaa acaaaatacc aaaaagctga actaaaacgt ttttgtgagg ggaaatagtc
    34081 agatatctct tgactttgag actggttttt agtaggttat aagtaatatc gaatgtcaat
    34141 gaaagagcga gtaagttttt gaaactttac aagagaggat catcgaggct gaaaatgatc
    34201 taaagtttca ttagcctaaa gacactctgg agatggacga gtgaaaagcc gtcccgcgta
    34261 cgagcgttat tcgtaagagg aaagagaaat ctttcaaaat taggtggtac cgtgtatgaa
    34321 tttcattcac ccttgttcat gatgaacaag ggtgattttt ttatttacat agctaagaaa
    34381 gggagagaaa actatgagtt tccaaagacc aaaaggaaca aatgatattt tgccaggcga
    34441 atcagaaaaa tggcagtttg tcgaagaaac agcacgttta ttattcagag attatcagta
    34501 taatgaaatg cgtacaccga tttttgaaca ctatgaagtg atatcccgaa gtgtagggga
    34561 tacaacggat atcgtttcaa aagagatgta tgatttttat gacaaagggg aacgccatgt
    34621 gacgcttcga ccagaaggta cagcaccaat cgttcgttct tttgttgagc ataaattgtt
    34681 cggcccagag tatgcgaaac cgttcaaaac tttttatatg ggaccaatgt ttcgctacga
    34741 acgccctcaa aaaggacggt tacgtcaatt ccatcaaatc ggagtagaag catttggatc
    34801 agaaaaccct gcaactgatg tggagagcat ggtgatggca cttgatttct tcaaacagtt
    34861 ggggatcacg caaatccggc tagtcatcaa ttcattaggt gataaagaaa cgcgtcaagc
    34921 ctatcgtcaa gcattgatcg actatctttt accttttgaa tcagagctga gtgaagactc
    34981 taaacgccgc cttcatgaaa atccattacg cgtattggac agtaaagaca aacgagatca
    35041 aaaatttgtg gcagaagctc catctatctt agattatcta agcgagcctg ctcaaaaaca
    35101 ttttgatacc gttgtttcca tgctggatgc attggctgtt ccttatgaaa tcgacagtaa
    35161 tatggttcgc ggactagatt attacacaca tacgattttt gaaatcatga gtgacgcacc
    35221 aaaaatgggg gcacaagcaa cgatttgcgc aggcggacgt tatgacaatc tagtggaaga
    35281 actaggtggt ccttcaacac ctggcttcgg atttgcaatg ggtatcgagc gattactctt
    35341 gactatggag gcagaggaag tagttatccc tgcatttaat gagttggatg cttacgtggt
    35401 cgcactcgga gacgaaacaa atatcgaagc attgaaagtg gtccaagcga ttcgaaactt
    35461 tggtttttca gctgaccgcg acttcatgaa ccgtaaagca aaagcgcaat ttaagacagc
    35521 agataaagcg aatgccaaat tagtattggt gatcggtgaa gatgagcttg ctagtcaaac
    35581 gattaatgtc aaagcaatgg caagccgaaa agaaaaaagt tttccattag aagagatata
    35641 tgaacatttt gatgaagtct atgatgaaat gactttattc gagtaggagg aaaaaaataa
    35701 atggcacaaa gaacgatcta ttgcggatta gtttccgaac aacaattaga acaaacagtt
    35761 acattaaaag gatgggtaca aaaaagacga gaccttgggg gagtaatctt tatcgaccta
    35821 cgtgatcgtg aaggaatcgt tcaaatcgtc tttaatcctg aaatcaacaa agaagcatgg
    35881 gaaatcgcgg ataaatgtcg cagtgaatac gtgatcgaag tgactgggga agttagaaga
    35941 cgcggagaaa atgcagtaaa tcctaaaatg gccactggtg aattcgaagt gattgcaagt
    36001 gaaatcgtga ttttgaatca agcaaaaacc ccgccatttc aaattgaaga tgatcaagca
    36061 atcagtgacg aaatacgttt gaaatatcgt tatttggatc tgcgtcgtcc aaaaatgact
    36121 aaaaacatca aattaagaaa tgcgacaaca aaagcgattc gtcattattt agatgataat
    36181 gactttctcg atatcgaaac accttaccta gggaaatcta caccagaagg cgcaagagac
    36241 tacctagttc cttcacgtgt ccatgctgga catttctatg cgttgccgca atctcctcaa
    36301 ttgtttaaac aattgctgat gaatgctgga ttcgaccgtt attatcaaat cgttcgttgt
    36361 ttccgcgatg aagatttacg tggagaccgt cagcctgaat ttacgcaagt agatattgaa
    36421 acaacgttct taacagcaga agaaatccaa acttacacag agggattgat tgcgaaggta
    36481 atgaaagaag ttcgcggtat cgaagtcaca ttgccatttc ctcgtatgac ctatgatgaa
    36541 gcaatggctc gctacggcag cgacaaaccg gatacacgtt ttgatatgga attgatcgat
    36601 ctaagcgata cagtcaaaga agtagaattc aaagtcttcc aaatggctct tgaaaacggc
    36661 ggtgtcgtga aagcattgaa tgctaaaggc gctgcagatc gctactcacg aaaagacatg
    36721 gatcagttag gtcaatatgt aggacaattc ggagctaagg ggcttgcatg gctgaaagta
    36781 gaagaagacg gattgaaagg tccaattgct aaattcatgg gagaagcaac agaagcaatc
    36841 attaaagcaa cagatgcaaa accaggtgac ttgctgatgt ttggcgcaga taaaagtgaa
    36901 atcgtcgctg ctgcattagg tgctatccgg acacgtttag gaaaagaatt aggactaatt
    36961 gatgaatcga aattcaattt cttatgggta actgattggc cacaatttga attttcagaa
    37021 gaagaaggtc gatatgtctc tgctcaccat ccattcacga tgccaaaaga agaagatatc
    37081 ccgttattgg ctgaagaccc tgcaaaagtc tatgcagaag cttatgatat cgtcttgaac
    37141 ggttatgaat taggcggagg ttctatccgt attcatactc gtgagcttca agaaaaagta
    37201 ctagaaactc taggctttac aaaagaacaa atggaagaac aatttggttt cttgttaaca
    37261 gctttagatt acggattccc tccacatggc ggtattgcat taggattgga ccgttttgca
    37321 atgttattag ccggagaaga taatattcgt gaagtcattg cctttcctaa aaacggaaaa
    37381 gcagcagatg tgatgagtga tgcaccaagt atggtttcgc ctttacaatt gttcgaattg
    37441 aatattgatg taacgaatat agaagaatag ccgataaaaa ggtaaaaagg atttgtcgaa
    37501 tacgcttctt ttgacaacct tgatttaatt gaaaaacact ttataatgaa gccataacat
    37561 ataaagcggg gcttttccat gaaaaaaaca ttcgaaaatt gggcttatca tgattacacg
    37621 accaagcttt cggtatctat cgtttatgcg ttgatcgcat cagtcgcatt gaatttcttt
    37681 tatcagccag gacatattta ttcaagtggt attactggtc tagctcagat tttgacgact
    37741 ttgtcaaaat cactgatagg attcgaagta ccggtatcaa caactttgta tttactgaat
    37801 atacctttgt ttgtattggc gtggttaaaa atcgggaaga agtttacaat tttcactttt
    37861 atcacagtca ctttgacctc cctttgtatc catattttac cggaaacagt cctatctacc
    37921 gatccaatca tctgtgcgtt gtttgggggg gctgtgatgg gactaggtat tgggctagcg
    37981 ttgagaaacg gcctatcatc gggcggtcta gatatcgtga gtatcacgat tcgtaaaaaa
    38041 acaggaaagt cgattggttc tatttcgatc tacttcaatg cattgatcgt ttttgttgcc
    38101 ggaattctat ttggctggca atatatgttt tacagtacgt tgtctatttt cgtcagtgga
    38161 aaagtaactg atgtcgttta tactaaacaa aagaagatgc aggtaatgat cgtaacgaaa
    38221 catccagaaa aagtagtaaa tgaaatccaa aactgtatgc gtagagggat cacgatcatc
    38281 aatgaagcag aaggtgctta taagcatgat aagcagacag tattgatgac gatcgttaca
    38341 cgatacgaac tgcctctact tagagaagca atgaaagctt ctgatccaca agcttttgta
    38401 agtattgcag aaaacgtcca gatacttggt cgtttctatg aggatggcat ttagatcgag
    38461 tttgacaaaa attcatgaaa tactgctgta gacagacgtt cgtttgtctg cagttttttt
    38521 ctaccatagt agaatcagca aagaaaaact gcttttttat ttgaaatttt cttaaagttg
    38581 aaaaaaagca tgtttatttt tttaaattta tgggatgata gaaaaaaata gtagagaaag
    38641 gacacgattc tacttgctat tttcacttaa tacccgtata ttacgaatga aaaaagttta
    38701 tgaaaagttg gaatattgaa tggaaaaaca aaaaaggtta aaaaacagcc gctacatcac
    38761 tgggtttgat gggctccgtt cattagctgt ggtgggtgtt atcttctatc accttctgcc
    38821 aactagtatg aaaggcggct atttgggcgt tccgattttt ttcgtcgtat ctggttattt
    38881 gatcacggat ctgctgcggc aggaatggga acaaaatgga aaaatcaata tttggcaatt
    38941 ttatgtcaga agaatgaaga gactctatcc ggcacttgtt tttatgcttg tgacagcttc
    39001 ggcttatatc actttgtttc agcgagggct gttaaacaat cttcgaggag ttgtggtcag
    39061 cagcttactg tatgtcaaca actggtggca aatcaataac ggtttatctt atttcgatcg
    39121 atttgctaat gagtcaccgt ttacgcatat ctggtcttta gcagtagaag gtcaaaacta
    39181 tttggtttgg ccgatcttgt ttgtattgtt gatggtattt gttcgtaaaa gaaaatggat
    39241 cttttatact gtgttaggta catccttcct ctccgccatc ttgatgatga tcttgtatac
    39301 accaggaggc gatccaaccc gtgtttatta cggtaccgac actcgtctgt tttctatatg
    39361 gttgggaagt gcgcttgcgt ttgtttggcc aagtacgcgt ttaaagaaaa acatcccgaa
    39421 acaagcgaaa aatattctga atatcgctgg aggaatctcg cttttggcac tgattctttg
    39481 cttcttcttt ttagatgatc attacaattt catctattac ggaggaatgc tcctagtgag
    39541 tctgttatgc gtagtgctag ttgcagtgac agcacacccc ggagcaagtt tgaatcgatg
    39601 gctgacaaat ccagtattta cttggattgg caaacgaagc tatggcattt atctgtatca
    39661 gtttccagtt atgatttttt atgaagcaaa ggtgactgat attgccgatc atgttttgtt
    39721 gcatacttta gtggaaatca cattgattct aatcatcagt gaactttctt atcgatatgt
    39781 ggagcgtccg ctagcacgtt ttgattatgg tcgtacattc caagtgatga aaggatggtt
    39841 cagcaaaccg atcatcagta agagcaaacc atgggttgtt ccaggcacat tacttgtagc
    39901 aattgcactc gttggtttcg ttacggcacc gaaaaacgct gtaacagccg atcaaaaaca
    39961 actgcaggca aaaatcgctg aaagtaaaaa attagcagaa gaaaccagaa acaattcaga
    40021 aaacaccact agttcggttg accaagcgat ccttgataag tacgagttgt ccaaggcaca
    40081 agggaataag gcacaaaaat tagaactaac ggcatttggt gattcagtta tgttggatgc
    40141 tgcaacggat ctacaagaac tttatccaaa agtagtggta gatggagatg tcggacgaca
    40201 attatatgcc agtgtaccgt acattgaaaa attaaaggaa caagatttgt tgaaagacac
    40261 tgttctgatt ggactaggaa cgaatggctc attctcggaa gctcagtttg atagtgtgat
    40321 ggatgcaatc ggtgatcgaa aagtatactg ggtcaatgtc cgagtaccga cgaagcgctg
    40381 gcaaaatgac gtgaatgcga tgcttgaaca gatgacgaaa aaatacgaca atatgacgct
    40441 gattgattgg tacagctaca gtaatgacca tagtgactgg ttttatgatg atcaagtcca
    40501 tccaaatcct gaagggatga cacattacat tcacttagtc agcgaagcct tgctaggagg
    40561 aagttcaagt acgaacagca gtgagacaaa cgtgacagaa tgatagctac aaaaaacttc
    40621 aggcaaaaac atttgttttt gcctgaagtt tttttatatt taccgtgttt ttaacattca
    40681 tatagattca ggaagcaaaa cggttgagtg tgtctgtaaa taacgttctt tttgttttcg
    40741 ggtgagtttt ttgaatgcag cttctgcttt tgttgcttcg cttcttgaag aaaatacttc
    40801 tgcatggatc atcttaacag gtcttctttt ttgaagacga gtatatttag caccagtacc
    40861 attgttatgt tcctctaatc gtctagaaag atcggttgta tatccaccat aaaatgtctg
    40921 atctttacag aataatacgt aaaaataatg ttcatttgcc atagagcatc tcacgaatct
    40981 ctgcagtgta ttctccatcc atattgtaag tgatcaatgg tggagcgact ttgaatccgt
    41041 ctaatttgcc ttctttgatt ccttcaacta acaaaatatt tgcctctttt ccaggttttg
    41101 ggtaaacaaa ttggatcctt ttaggtgcaa ttcgatagtc ttccatgacc tgaatgattt
    41161 ctaagaaccg atcaggacga tggaccatag taaagcgtcc atttgttttt agtagcttac
    41221 ttgaaatatc gatcacttct tctaacgtcg tatgaatctc atgacgggcg attgccaggt
    41281 gtttattagg gttcgtttta tttgttggca gccctttgaa ataaggtgga ttacagacga
    41341 ctaagtcaca agaattatgt cgaacgacag ataagctgtc tttcagatcg atggtgtgga
    41401 cagtcatttg ttcatttaaa tggttaagcg cgatactacg tcttgccatg tctgctagac
    41461 gaggttgaag ttcgatagcg tcgatagccg cctgggttcg tttacttaaa aaaagtccaa
    41521 ctgcgccatt tcctgcacaa aggtcaacaa tcttcccttt tttaggtaca cgggaaaagt
    41581 gagcaagcag cacagcatct aaagaaaaag aaaacacttt ggaactttgg ataatcttga
    41641 tatcttctgc gtataattgg tcgattcgtt cgtcttcgtg aagcatggat tccctccaaa
    41701 atgaaaaaaa tagtcgtctg tattatacta tttttattaa aaagaaccaa ggtggcttgt
    41761 caaacctcta ttttttatgc atactatgac cataagaagg gaaaggaaga gactatgttt
    41821 tatcgattta tgcgcggggt agttcgagta ctattagcct tagttaatgg aaatgcccgc
    41881 tatgagaata aagaagtttt accgcaagat gagaattaca tactagttgc tccccatcgt
    41941 acttggtggg acccattata tctggcggta gctgcagcac ccaaggaatt cagctttatg
    42001 gcaaaagaag aactgttcaa aaatccagtt ctgcgattta tattaactcg atccaatgct
    42061 tttccagtta aaagagaaaa tccaggacca agcgtcatta aaacacctgt aaagattttg
    42121 aaatcgacaa atttgagttt gatcatgttt cccagtggga caagacatgc tacagaactt
    42181 aaaggcggaa tggcattgat ttccagaatg tctaaagcga aaatcgtgcc agctgtatat
    42241 caaggtccgt tgacacttgg ggatctgttc aagcgtcaga gagtgacagt gcgtttcggc
    42301 gaaccaatcg acttatcaga tatcaagaaa acggataaag aaggtatggc ggagatcgaa
    42361 cgacgaacac aagcagcttt tgacaaattg gacaaagaag tgaaccctga tttcaaatat
    42421 gaagtcaaaa aaaaataaaa tgataagttt atgcgtgggc ggtcttttga atctactgtc
    42481 ctgttcagtt cagacttttt caatgaaaac gagggagaga cgtcaagtct gccctctttt
    42541 tgcgttccaa tatgtaaaat agaagacagg tgtcatgtaa tggatcaaag tgttataata
    42601 attagaagga cattttttga ggtgattgaa tgcgtttttt acatacagcc gattggcata
    42661 tcggaaaaaa actacaaggt tatgacttga ttgaagacca gcgtcatgta ctatcttcta
    42721 tactagatat cgcgaaagaa gaaaaagtag aggcaatcgt gattgccgga gacttatatg
    42781 ataggagtgt cccagctgtt gatgccgtag aattatacaa tgaattgatg gtcgattgga
    42841 atttaaagga aaagatcccg atttttgcta tatcaggaaa tcacgatagc agtacgagac
    42901 tttcagcggg tacgccatgg ttcagttatt cgaactattt tttgcgaaca cagctagcag
    42961 aagcatttga accagtcgag tttaaaaacg tacaattttt ctttcttcct tactttgaac
    43021 caattgcagc gagattatat tttgaagatg acaatatccg aacgatcaag caagcggtag
    43081 ccaaggtgat agataaaatg caggagctat ttgattcaga aaaaaaacaa gtattgatta
    43141 gccatttttt tgtaatggga agtgtaaaat ctgattcgga aacgaaaatc gaagtaggcg
    43201 ggttggattc gatccctggt gaactactta caccgtttga ttatgtagca ctaggacatt
    43261 tgcataatca agctgctttg agacaggata atgctcgcta tagcggttcc ccattgaaat
    43321 tctctttatc tgagatcaac caaaaaaaag gcgtatggat cgttgacctg acaacaggcc
    43381 tggagttgaa ttttagggag atcgaacccc tgcatgatat tgaagaaata acagcgagtt
    43441 tcaaagaatt gctggatcct gccttttatc agtccgtcaa tcgggagaat tacatctata
    43501 tctatctcac tgatagaacg atcatcccga atatgatgaa ccagcttcgg caaatatatc
    43561 ctcgtatttt ggggattgaa cgggcaaacg gtcgagcaga acaaacaaag cgcaaacgaa
    43621 aagtactaga aatgaaagat cctcaaaaat tagcagaagc attttttagt gaaatgacgg
    43681 aagagcagct aactgcaaac caagaaaaat ggctcggaga aacgttgcaa gaaatcatgg
    43741 aaatggaatg atcaagaatg aaaccgaaaa aattacaact taaaaatttt ggtcctttta
    43801 tcaatgagac cgtagatttt tcacgtttga cagaagcacc gctttttttg atcagcggga
    43861 agacaggagc tggcaaaacg acgatttttg atgggatcac cttcgcactt tttggtgaaa
    43921 cttccggaag acttcgctct ggaaaagaga tgcgttcttt gtttgctaca ccagacgaag
    43981 aaacaagcgt gactttttct tttgaacatc agcagatgag ctatgaaatc gaacgaaaac
    44041 cagaacaagt tttagcaaaa cgaaaaggaa acggaacaaa aaaacaggca gccaaagtaa
    44101 cgttgaccat ttttgatggg aaaggaaaag aaataaaaca aatcacaaaa cgtacagaag
    44161 cagatcagtt gatcagagaa ctgatgaatc tcgatgcgaa acagttttct caaatcgttt
    44221 tactacccca aggagaattc cgtaattttt tggtttcatc aagcagtgat aaagagactg
    44281 ttttacgtca tttattcggt actcaatttt tccaacagtt caatgagcga ttaaaagaaa
    44341 aagcaaaaca acagcaacaa aaattggatc atttagagca agagctgcat cttctgcaaa
    44401 aaaggtttgt tttagtcaaa gaagaggaac cagcagcagg gtttgaagcc gttttagccc
    44461 aatggacaaa ccatcaggca atactcgaaa aacaaatcac ggaagaaaaa gaagctctaa
    44521 cggaacttca ggaaaaacaa aagcaactag aaaattccta ctatcgtttt gaaagcttga
    44581 aaaaaagtta tgaagaaaaa acgatattaa taaacaagca aaaggaatta gctgctcaaa
    44641 aagacgaaat agaggaaaag aaacgatgga ttgggtatta tgaatttacg caacgattag
    44701 tcgaaccaat ccagcgccta caagaaataa aagttgaaaa aatagaactg tcacaaaaaa
    44761 gtagtgagca aacaaagaaa ctagcgatga ctacgcagaa ttatgaagag tggcaaaaaa
    44821 atgaagcaaa ccgcgtacgc cgacaaacaa aaatcaaaga agctgctcaa aaactgcaaa
    44881 acaaacagat gatggtgcca attgtcgaat ccttacgtag taaaaaacaa gacgcagaac
    44941 aattaacaat ttcattggat aaagaagaat tgaatgccga gaagctgtat caaaatctcg
    45001 ctatctatca ggaaagaact cgaattcttc aagaaaattt actggatcag gaaaaactgc
    45061 aagaagatcg tcttgctttt ggacagttaa aatatctaga tgaaaagcag caagaaatag
    45121 aaaaacaaaa agcaaaagac actgggtcgc tcgcacagat aagagaagaa ctcaaaaaag
    45181 caactgaaga aataacaaaa aatagtcagc tattgaaaga acagacagaa caagcagaac
    45241 agctgaaaag ccaatgggca aagcagcaaa ttgctcgtct tcagcttttt ttgcgtccag
    45301 gggagccatg tcttgtttgt ggatcaacgg aacaccccat acaacaatta aaacacgaac
    45361 agccaaataa ggaagatatc ttaaaagtag aacaacaact ggcagaagct gagtctgctg
    45421 tagaaagaac tcagcaaatc attgccaaaa gtgaagcaca tgaaaataat cttcaacaac
    45481 aattatcaga tttagaacaa aaaatcatgg caacgaatat acaattggct gaacaagaag
    45541 aacacttgca acaggctttg aaaaaggcat ttcctgagat aacagaacaa aaaacaaaaa
    45601 caactcttgt agtaatcgaa caatctttag atagacgaaa aagcaagatg gccaatgcaa
    45661 gaatagagct ggaagaaata agacaacaag cagaaaagct cgaacaacaa ttagctgtct
    45721 acaaacagca gcagacagaa aaaaataatc agctccagca actgcaagga gaaatcaaaa
    45781 gtttagaaca acaattgcaa gatacggata gtaactgttt agaagaggag atacgacgac
    45841 ttgaaaaaca gttagaagaa gactcgaatc aactagctgt tgagcagaaa gcaggagtgc
    45901 aattgttgca agatcttcat acgcttcgga cacagcttga tcttcaacat gaacaaatag
    45961 aaaaaataga tgaaaaaata gaaaaggaac aagaaagaat agctaaaaag ttagaaagcc
    46021 agtcgtattt ttcaacaatt gaagaaatct gtgcatttgc acaacagcaa gcacaatata
    46081 aagaaaacca acgtacagtt acttcttatc atgaagagca attagtgaca gctgaccggc
    46141 tgcgtcaatt agaaaatatc cagctaactg agacattccc agatacagaa gagatcagcc
    46201 aacagttaga agaaataaga cgtatgatcg aaataaagca agaaaagata tacggtttac
    46261 aagaacaaaa aattagtaat caaaaaatct acgatgaatg tctttcgatt tatcacacaa
    46321 gccagacgaa attagatgag cttacgcaat tacagcagct ttcgcaaaca ttcaacggag
    46381 aaaatcctaa gaagactagt ttggaacggt atgtgcttca ggtttatctt caagaagtgt
    46441 tacaagtagc aaatgatcat ctccaacgtt taacgaaaaa tcgttatcaa tttgagctag
    46501 cggataccat tggcagttat cgagggaaaa ccggattaga aatcaatatc tatgatgatc
    46561 aggcaggtat gtctcgaagt gcgcataccc tgtctggcgg tgagagtttt attgcagcac
    46621 tttctttagc attagctctt gccgatgtga tccagacaca agctggtggt gtagcaatcg
    46681 atgcattgtt tattgatgaa ggatttgggt cattagatga agaagctttg gaaatggcaa
    46741 tggaagcttt agaaacgatc gaaagtgaag gaagaatgat cggcatcatt agtcatgtaa
    46801 gagaattgaa agaacgagta ttacaacaaa taagaattga tacagaaggt agcggacaaa
    46861 gcaaaatacg ctatcaactt ggataaaaag ggagattggt aactatgaaa tggagtattc
    46921 cagaaaaagt cattgcacgt ggacgtcaat acttgcaaga aggccgagtg ttatcggttg
    46981 tccctgacca agaagaaaac gtgtggcatg cagaagtctt gggcagtgaa ttgtatatgg
    47041 ttgatttgga tggtacagca aaagagatag attactgcca atgtccctat tgggaggaac
    47101 ataaatattg taagcataca gttgctgtgg aactttattt acggtcgaaa aatctatctc
    47161 gtatcatgaa aaaagaccag ccaatgctag aaccggtaaa agcaacaagt gagggagaaa
    47221 tcattgcaaa aggtttttct cgtctaaaaa cacaaaacaa tcctcaagcg gtgcaacctt
    47281 tgattattga ataccaagta gaaacgattg agactaatca gtatcaccca gagctttcta
    47341 ttttggcact ttacttgcgt atcggttcat tggcatcacc gaaaaaaaca tatatcgtaa
    47401 aaaacatcta tgatttttta caagcttaca cagaaaaaga aacttacact gttaataaac
    47461 agtaccagtt tcgtctagat aaacgggcgt ttcaaaaaga agatcaaaaa atactcacgc
    47521 atcttgcagg aagtgctcag acgcatcaat tattaggtac gaacggctta caagtcaaag
    47581 gaaaactaga taaaagatat ctgcttctcc cagtagaaca agcccgcttc ctgttagagc
    47641 agatgaatca gactacacgt ttgcatctgc agataaatga acaaaacgat caaggaatac
    47701 aattttccga tcaaaacaaa cctttatcat tcaaagtgga aaaatttgga gaagattatt
    47761 tgctgcaagc attgaatgat tttgattttt tctttatgca ttatcagtgg ggagttatcc
    47821 aagggacatt ttattctctg acgaaatatc aacaaacggt ttatcaaact tggaaacaat
    47881 tgattcgccg attggagaaa ccagaagtag tgttcaagaa aaaagaatta ccgtctttgt
    47941 ttaaagagat tatccctctg ctgtcagaga tcggcgacgt agctattgcc cctgcagtag
    48001 aagaggaagt agcagatatc cccgtcgaat ttattttctt tttccgaaaa gcaaaaggaa
    48061 aaatccaagc tcgagtcgat ttcgtctatg gtgaagtcat ctattcgact gacccgaagc
    48121 atgaggtctc gacagaacag gcttttcagg tgttacgtga cacagtagaa gagaaacgag
    48181 tacttgatct attcagaatg tatcgctacc agaaaaatga aactggttat gaacgtccat
    48241 tgccaagcgg ggaagaacta tatgcctttt tccgcacaga actatctgtc ttccgtcagt
    48301 ttggtgaagt cagacttgga agaaaattgc gagagttgta tttagatgcg cataaacatc
    48361 aaccgaaaat cgaagtaacc gagaccggtt catggctcga tattcgattc gatgtgacag
    48421 ggatagagga gcaagaaatc gatcatgtcc tgcaaagctt gctgagaaat gatgcttttt
    48481 atacattaga aaatggtcag gttctttctt ttgattcaga agaatttcaa caaacaagcc
    48541 aagtgttgca acaattacgg gaatcgatac gtacagaaga aggaacgatc catgtaccta
    48601 aaaatcaagg attgatcatt caaaatcaat tggaaaaaag caatgctact tttagtgaat
    48661 cgttccaaac gatggtacaa gatctcattc atccagaaag ataccaggca cagcttccca
    48721 aaggattgaa tgcaacgatg agagattatc aaaagcaagg ttttcgatgg ttgaagatgt
    48781 taggtcatta tcagtttggc ggtattttag ccgatgaaat gggacttggg aaaacactac
    48841 agacaattgc ctttttactt tcggaaaaag aagaacggaa aagcttctct gctttgatcg
    48901 tagcgcctgc gagcttaatt tacaactggc aggcagaggt gagaaaattc gccccttctt
    48961 taagtatcca agtaatcaat gggaataaaa aagaacgtga agaattactg gctaaagaca
    49021 cagatatccg agtgacttct tatgccagct taagacagga tctagctaat tatcagtcgc
    49081 aaaaaattga ttatttgatt cttgatgagg cacaaatggt caaaaatagc agtacgaaaa
    49141 ctgcacaagc attgagagaa ttagctgtac ctcagcggtt tgcactgagt gggacaccaa
    49201 tcgagaataa tttagaagaa ttgtggtcat tgtttgcaac gattatgcca ggatttttcc
    49261 cgactaaaac aaaattcagg gaacttgccg cagaagaaat tgcgcaaatg attcgaccgt
    49321 ttattttacg gcgagataaa caaactgtgt tgaaagactt gccggaaaaa acagagatga
    49381 atctatattc tgcacttaca gaagaacaaa aaactgttta tttagcttac ttgcgtcaaa
    49441 tgcgtgaaga aatcacatca atggattctg aagcattcaa gaaaaaccgg atcgggatac
    49501 ttgctggtct gacaagactg cgacaaattt gctgcgatcc tcgtctgttt attgaagatt
    49561 accaaggcgg ttccggcaag ctcgaacaag taaaagatct actggtagct gccaaagaga
    49621 acaaacgaag agttttgctg ttttctcaat ttacagggat gctgactatc ttgcaagaag
    49681 agttagccga gctagggatc tcgacatttt atttaagagg cagtacgaaa ccacaagatc
    49741 ggttaagtat ggtagacgca tttaatgctg gcgaaaaaga tgtttttctg atttccttaa
    49801 aggctggggg aactggattg aatctcacgg gagcagatac ggtcatcttg tatgaccttt
    49861 ggtggaatcc tgccattgaa gaacaagctg ctggacgtgc acatcggatc ggacaaaaaa
    49921 atgtggtaga agtgtggcgt atgatctctg aaggaacgat tgaggaacgc atggattcac
    49981 tccagcaaga aaaacgcgaa ctgttccaaa aagtgatcca aggtaatgaa gaacagttga
    50041 cgaaactgac tgaagaagac atccgcatga ttttaagtgt aggagaaatc gaggagtaag
    50101 agactcgaaa aagcgttcag taatgggtaa gaaagccttt ctaaagaagc gattgtttat
    50161 ttgatttaaa aaatagcaag gaggctggga ttccatttta tcccagcttc cttgctatca
    50221 tttttttatt tctattttta aaggttatac aaagattgtc ttcattgtct ctaacagctg
    50281 atcaaaagaa gaaatctcga tatcgcaaat tggagtctgt tcaattttgc gttctcgatg
    50341 gttgaaccaa agcgcttgcc agttagcact ttttgcacct agaacatcat tgtcgtaatt
    50401 atcgccaacg tataaggtct cttctggaag catatgaaat tctttctcag ccagctgaaa
    50461 aatctctttt tctggttttt ggaaacctgt tgcttgagaa atgagcatat tgcgggaagg
    50521 aatccaattg ttcaattgaa gttgcttcag tttttttgtc tgatggtcag tcggcccatt
    50581 tgtgataatt cctaatggga cattcttttc cttcaagaaa tcgagggttt ttttgacctc
    50641 ctcatgcaag cagatattat caagttcttc ctcgtaaatt ttttgaaata agaggccatc
    50701 ttcttccgta atatgagggt agtctaaatc ttttagtgat tggctgatcc gatgtgctcg
    50761 catatattct agtgtccaat ctcctgccat tacttttggg aagttttcat cactatgatg
    50821 gcggaaccgg atatatagtg gatgcatatc tgcatctgaa acaagcggaa cgactctttt
    50881 gaccgcgttt cgaaatggct gctgctgatc atacatcgta tcatcaacat caaacacaac
    50941 tgatttaatc aaaattttac acgcctttcg gttttcattg attttttcac aaatccttga
    51001 ctattgtaac agaaaaaatt gtctaaaaaa gctgataaat aaaggttttt cttactatat
    51061 ttctatttcc ttgacagaat aaggttttta tatttcagtt tttttcacta aaaatcgtga
    51121 aacacgtcaa taagtttgat gaacaaatta atcattatgg tagaagtcga ttgatttatt
    51181 ttaatgcaat ctctgtggaa ttttcgaaag atttattttt ttaata
//
LOCUS       DAZKLM010000007        50742 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3764
            SAMEA115706913-rid22582563.denovo.007, whole genome shotgun
            sequence.
ACCESSION   DAZKLM010000007 DAZKLM010000000
VERSION     DAZKLM010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706913
            Sequence Read Archive: ERR13196647
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 50742)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 50742)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287823.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287823.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,004
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,849
            CDSs (with protein)               :: 2,849
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 41 of 96
            Pseudo Genes (incomplete)         :: 52 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 15 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..50742
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706913-rid22582563.denovo.007"
                     /isolate="VRE3764"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(262..1989)
                     /locus_tag="ACG842_000358"
     CDS             complement(262..1989)
                     /locus_tag="ACG842_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate binding
                     protein"
                     /protein_id="HFQ0246927.1"
                     /translation="MNNLLNYQLPVANWVENITEWFTTTFSGLFSFLQTIGQAVMSGI
                     TNLLLVIPAPLFILLLTVAAFFISKKRPGLTLFTLIGLWFIYNQGLWNDLMNTVTLVL
                     LSSVISIIIGVPLGILMAKSSKAQSIIKPILDFMQTMPGFVYLIPAVAFFGIGMVPGV
                     FASVIFALPPTVRFTNLGIRQVPKELVEASDSFGSTSRQKLFKLELPLAKSTIMAGIN
                     QTTMLSLSMVVIASMIGAPGLGRGVLSALQRAQVGNGFVNGVALVILAIIIDRFTQHL
                     NQPNNKKAAGATTQKSKKRQGLIIGAVVVVILGAIGIGSFSSAKETKRINLSYVEWDT
                     EVASTNVVGEVLKQMGYDVTMTPLDNSIMWKSVSNGESDAMVSAWLPKTHGSQYAQYK
                     DQVEDLGANLTGAKVGLAVPAYMDVNSIDELTDQAGKKIIGIEPGAGVVTAAENTIQK
                     YDNLKDWKVETSSSGAMTVALGQAIKKHEPIVVTGWTPHWMFAKYDLKYLEDPENGMG
                     SEEQIHTMVRKGLKEDQPEAYKVLDNFHWSEKDMEKVMLEINNGKDPQQAAKDWIKEN
                     QELVESWKK"
     gene            complement(1982..3175)
                     /locus_tag="ACG842_000359"
     CDS             complement(1982..3175)
                     /locus_tag="ACG842_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein"
                     /protein_id="HFQ0246928.1"
                     /translation="MVKVEVKHLTKIFGKKTQAALDMMNDHQPKTEILKKTGATVGVY
                     DVNFDVKEGEIFVIMGLSGSGKSTLIRLLNRLIEPTSGSIYIDGEDVSKLSKEELREV
                     RRHKINMVFQNFGLFPHRTILENTEYGLEVRGVPKEERQEKAEKALENSSLLSFKDQY
                     PSQLSGGMQQRVGLARALANDPEILLMDEAFSALDPLIRREMQDELLDLQANVQKTII
                     FITHDLNEALRIGDRIALMKDGEIMQIGTGEEILTNPANDYVREFVEEVDRSKVLTAQ
                     NIMVPALTTNIESDGPNVALTRMRNEEVSMLMAVDRKRHLKGIITADQALEARKQKRP
                     LIDFLDENVTVIGKDMVVSDIFNIIYDSPTPLAVVEDGKLKGVVIRGSVIEALAETSE
                     VSEHE"
     gene            complement(3362..4006)
                     /locus_tag="ACG842_000360"
     CDS             complement(3362..4006)
                     /locus_tag="ACG842_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFQ0246929.1"
                     /translation="MSGKKKIVRPRYQQIAVDLAERIVENRYKVGEKIHARSTLASTY
                     NASPETTRKAINVLVDLGIMEVRHGSGAFVASKEKAKDFLLQYQDVQSIQDTKAEIMA
                     SVEKQQDELNHFSQLLDQLVNQTKRIHKMNPLLPFELSLSEKAMHLDRSISELNIWQA
                     TGATVIAISHNEELLVSPGPYAKFTAGDIVHFIGNEYTLQRMTNFFYPEHQLAE"
     gene            complement(4100..4234)
                     /locus_tag="ACG842_000361"
     CDS             complement(4100..4234)
                     /locus_tag="ACG842_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HFQ0246930.1"
                     /translation="MATFLLSVLIFGSAGAIVYTRLKSGKNCDDCQTACPVKKEQIKR
                     "
     gene            complement(4236..6368)
                     /gene="feoB"
                     /locus_tag="ACG842_000362"
     CDS             complement(4236..6368)
                     /gene="feoB"
                     /locus_tag="ACG842_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HFQ0246931.1"
                     /translation="MNIALVGNPNSGKTSLFNLLTGSNQYVGNWPGVTVEKKEGSLKK
                     NPNIHIQDLPGIYSLSPYTPEEVIARDYLVQEQPELIINIIDATNLERNLYLTTQLLD
                     LDIPMIIGLNMMDVIKKEGKKINLEKLSYGLGVPVLPISVLKKQGIEKLIEKIQESAK
                     KRPSIPVPCTYDPRLEAALHEIEDVLGEQSGKKRWYAMKYFERDQKVREDYEIAVSQQ
                     KEIEQLIQLTEKLLDDDSETILVNERYEFITQLCTLCVVSNDSFQLSMSDKIDQIATN
                     RWLALPIFAFVMWLIYYLAIQTVGTMGTDWINDTLFGTWLPEHVGRLLAEWQVAGWMQ
                     ELILNGIIAGVGAVLGFLPQLIILFLCLSFLEDCGYMARIAFVMDRLFRKFGLSGKSF
                     IPMLIATGCGVPGVMASRTIENEQDRRLTIMVTTFMPCSAKLPIIALVAGAFFPHQSW
                     VAPSAYFLGMTAIIFSGISLKKTRLFAGDTAPFIMELPAYHFPQWSAVLRQTYDRSKS
                     FVKKAGTIIFVSSIVIWFSSSYNFYAQPVSEDQSILAFFGRILAVVFQPLGWGNWQGT
                     VAAITGLVAKENIIGTFGILFGHAEVSENGREIWQVLQHTYTPAAAYSLLAFNLLCAP
                     CFAAIGAIHREMNAKKWTWIAIGYQCGLAYLVSFVIYQLGHLLEGGQVALGTYLAILL
                     MIGLVYVLFRQPKSKNQFYTILSLEGEE"
     gene            complement(6365..6838)
                     /locus_tag="ACG842_000363"
     CDS             complement(6365..6838)
                     /locus_tag="ACG842_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HFQ0246932.1"
                     /translation="MQTLAQAKQNFVYQVERIETEEKTKRHLQNIGIVAGSKIVVVNL
                     SGNNAILLVKSHRVAVSREILGQIFVNKKTASSESWTSLDQLKAGETGIVVAIHGQGA
                     VKRRLMDMGLTRNVKIVIRKLAPLGDPIELTVRGYELTLRKSEAELILIQREEKE"
     gene            7239..7463
                     /gene="nrdH"
                     /locus_tag="ACG842_000364"
     CDS             7239..7463
                     /gene="nrdH"
                     /locus_tag="ACG842_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HFQ0246933.1"
                     /translation="MNVKIFSKNNCMQCKMVKRFLTENQIAFEEVNIDEQPEAVDWLK
                     AQGFQSVPVITSDAETVIGFRPDQLRKLAS"
     gene            7622..9781
                     /gene="nrdE"
                     /locus_tag="ACG842_000365"
     CDS             7622..9781
                     /gene="nrdE"
                     /locus_tag="ACG842_000365"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000523276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HFQ0246934.1"
                     /translation="MSLKNLQDVSYFKLNNEINRPVNGQIPLNKDKEALVAFFEENVK
                     PNTMTFLSIMEKINYLIDQEYIEAEFIQLYSPSFIEKLYQFLDEQHFSFKSFMAAYKF
                     YSQYALKTNDGSAYLETYEDRVAFNALYFANGDEELALALADEMIHQRYQPATPSFLN
                     AGRKRRGELVSCFLTQVTDDMNSIGRSINSALQLSRIGGGVGITLSNLREAGAPIKGY
                     EGAASGVVPVMKLFEDSFSYSNQLGQRQGAGVVYLNVFHPDIIAFLSAKKENADEKIR
                     VKTLSLGVIVPDKFYELTRKNEDMYLFSPYSVEKEYGVPFSYVNITAEYDHLVANPNI
                     RKQKIKARDLENEISKLQQESGYPYVINIDTANRENPIDGKIIMSNLCSEILQVQTPS
                     VINGKQEYEVLGTDISCNLGSTNIVNLMQSPNFGQSVRTMTRALTFITDASDIDVVPP
                     IQNGNKLNHTIGLGAMGLHTYLAKEQMEYGSEDSLDFTNIYFMLLNYWTLVESNNIAR
                     ERKQVFHNFEKSAYADGTYFEKYVTGEFQPKSEKVKALFEGIFIPTAEDWNALKQAVM
                     KDGLYHQNRLAVAPNGSISYINDTSASIHPITRMIEERQEKKIGKIYYPAPYLSNETI
                     PYYTSAYDMDMRKVIDVYAMAQQHVDQGMSLTLFMRSEIPEGIYEWKTTTKQTTRDLN
                     ILRHYAFHKGIKSIYYVRTFTDDAEEIGSNQCESCVI"
     gene            9831..10796
                     /gene="nrdF"
                     /locus_tag="ACG842_000366"
     CDS             9831..10796
                     /gene="nrdF"
                     /locus_tag="ACG842_000366"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005971 - ribonucleoside-diphosphate
                     reductase complex [Evidence IEA];
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HFQ0246935.1"
                     /translation="MSETYYEAINWNEIEDIIDKSTWEKLTEQFWLDTRIPLSNDLDD
                     WRTLSQLEKDTVGHVFGGLTLLDTVQSESGMDQLRKDVRTPHEEAVLNNIQFMESVHA
                     KSYSSIFSTLNTKKEIEEIFDWTNTNPYLQKKAERINEIYKHGTPLEKKIASVFLETF
                     LFYSGFYTPLYYLGNNKLANVAEIIKLIIRDESVHGTYIGYKFQLGFNELPEAEQETL
                     KDWMYNLLYELYENEERYTEELYDPIGWTEEVKTFLRYNANKALMNLGMDPLFPDTAN
                     DVNPIVMNGISTGTSNHDFFSQVGNGYLLGHVEAMKDDDYLIGLE"
     gene            complement(10885..12024)
                     /locus_tag="ACG842_000367"
     CDS             complement(10885..12024)
                     /locus_tag="ACG842_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFQ0246936.1"
                     /translation="MQSRFKQSKLFFWTVELVLTIIGVYFILQMPNIFSPILKMLSAV
                     FLPLLVAGFIYYMFDPIVVFLEKHRIPRVFGFLLTFLVVIILVVLTVMNVVPQLMEQT
                     IQLTQSLPMYADETSKWLNELAQREEFKNFNLEEQLASANLSLSNLFNIILVSLSASV
                     SKIISFMMQFFILLFTVPFILLFMFKDGHKFIDALSHFFPKAIRKELRQTVKELNETL
                     SAYISSTILDALIIGVMSFLAMRIFDQPYSLLLAVICGITNIIPYVGPFIGAVPAIIV
                     GLFISPFQALYMALSILVIQQLDGNLIKPLLFGKSMNIHPLTIILVLIGAGSVAGILG
                     MLICIPVYAVIKTIILNIRKIYLLRRMEHVSEKLDSPKVLEETSE"
     gene            complement(12333..13046)
                     /locus_tag="ACG842_000368"
     CDS             complement(12333..13046)
                     /locus_tag="ACG842_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HFQ0246937.1"
                     /translation="MNDMVLTIPEIILRLGIAMLFGGAIGFERQYKNRPAGMRTHILV
                     CMGACIIALIQVEIASGALRDALEYPQLAGTIRSDEARLIAQVVSGVGFLGAGTIIVT
                     KQSVTGLTTAASLWAIAGLGIAIGMGYYSIAIISFIGIAIALTVVKRVIHVPTTKKLE
                     IQFIHRKETKDFLTDYFEEKNIVIEDVNFDVKFVDDYRVYKNIYTIDLPKGLTYADVI
                     EELSVHKNITKLHLVSIAQ"
     gene            complement(13300..14001)
                     /gene="nagB"
                     /locus_tag="ACG842_000369"
     CDS             complement(13300..14001)
                     /gene="nagB"
                     /locus_tag="ACG842_000369"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HFQ0246938.1"
                     /translation="MEIIRVKNAEEGGKKAFEIIKEGMNKGAKVLGLATGSTPVTLYK
                     EMTSSDLDFSEMTSVNLDEYVGLGGSDEQSYRHFMDVQLFDKKPFKETFVPDGKAEDL
                     EAACKKYDQIIDAHPIDIQILGIGQNGHIGFNEPGASFDGTTSVVDLTESTINANKRF
                     FDKAEDVPTKAVSMGIGSIMKSKEIVLMAFGESKADAIKGMIEGPVTNHLPASVLQNH
                     DNVVVIIDEEAASKL"
     gene            14235..15767
                     /locus_tag="ACG842_000370"
     CDS             14235..15767
                     /locus_tag="ACG842_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HFQ0246939.1"
                     /translation="MSILTLNNITQQFGDKILYEGVDLQLNAGEHLGLIGQNGAGKST
                     LIKIITGEILPDDGQIHWQKNIHKGYLDQYVEVDETLTIEEFLKTAYAKEYEKEEKIA
                     FLYQTYSETFDDSLLEKAGKLQTELDQGVFYQIDTLVAEMSSGLGIDVLGLDSLLKNL
                     SGGQRSKVILAKLLLENPDVLILDEPTNHLDDQHIQWLTQFLQDFDGAYIVISHDQEF
                     LDQITTHIADIEFGKITKYTGHLKQALKQKEQNRESYLRQYHAQQKQIEKTEAYIRKY
                     KAGSRSTMAKSRQKQLDKIERLTPPASASKAVFDFPFSPIVTTLAVETTDLVIGYDRP
                     LLAPINLTIRFGENIAIRGFNGIGKSTLLKTLIGEIEKISGDFHFPDNTKINYFSQDL
                     YWENPLETPLQYLSSQFPKATIKELRRQLAKAGLVNQLASEPLTTLSGGEQTKVKLAQ
                     LTMNQGNLLILDEPTNHIDQETKESLQDGIQKFPGTVIIVSHEQEFYQDLVQRVIEIE
                     PK"
     gene            complement(15958..17160)
                     /locus_tag="ACG842_000371"
     CDS             complement(15958..17160)
                     /locus_tag="ACG842_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFQ0246940.1"
                     /translation="MKSNEKKPLTTSLILLLSITCGVVVANMYYIQPIGTKVAASLSV
                     STSAVGILTMLTQLGYALGLLFLVPLGDVVDRPKLIIRMAALSAVSLLAAFFAPSFTL
                     FACASFLIGLLSIVPQIIIPYGAVLAGPKARGKVMGTLLSGLLVGILLSRTVSGIVAS
                     LFSWRMIYLFALCAVGILTLLLYWKMPRSVQQSSTAVSYLDSLKSLPHLVASQRLLRE
                     AAVSGFFMFGTFSIFWSTLIFYISSPAYNWGTFEAGILAIFGLSGAVAAPIVGRLADS
                     YSERKIVYMGLVMQTISFIALLVGGSHLAILLLAIILLDVGNQFGQVANQTRVQGLGE
                     AASNRNNTVFMFMYFIGGAAGSLLGTTMWQQYGWLGVTVSGLVFQGCAFFFQTVVFKG
                     KRNLENKK"
     gene            complement(17242..17931)
                     /locus_tag="ACG842_000372"
     CDS             complement(17242..17931)
                     /locus_tag="ACG842_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HFQ0246941.1"
                     /translation="MKKKIIRLILISLGIILILVVGYRLYLEYQTDIQLFLNPRASKA
                     ELLAHIRSHGTFASILLIVLTAVMCAVPGIPTSVVGVIAGLSFGPLTGAIINILGNAL
                     GNITAIFLMHRLKFLDKKTETNHWVQAIRQMKHPKIGVMLGYMIPVIPSSMVNFAADA
                     MKLPLQQIILSIVIGVIPSSVLYACGGEALFHGYNKTAVTLVASVTVLVGLVVFIYKD
                     RKKKQGKQKHK"
     gene            complement(18037..18405)
                     /gene="rplL"
                     /locus_tag="ACG842_000373"
     CDS             complement(18037..18405)
                     /gene="rplL"
                     /locus_tag="ACG842_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HFQ0246942.1"
                     /translation="MALNIENIVAELKEATILELNDLVKAIEEEFGVSAAAPVAVAAA
                     GGAAAAEEQTEFTVELTAAGDQKVKVIKAVREATGLGLKEAKAVVDGAPAPVKEGVSK
                     EEAEELKAKLEEVGASVTVK"
     gene            complement(18458..18961)
                     /gene="rplJ"
                     /locus_tag="ACG842_000374"
     CDS             complement(18458..18961)
                     /gene="rplJ"
                     /locus_tag="ACG842_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HFQ0246943.1"
                     /translation="MSEAIIAKKAAIVDEVSEKFTAAASVVVVDYRGLTVDEVTRLRK
                     QLRDANVEMKVIKNSILSRAAKKAGLEGMDEVFTGPTAVAFSNEDVVAPAKIMDEFAK
                     EAKALEIKGGIIEGKVSSLEEITALAKLPNREGLLSMLLSVLQAPVRNVAYAVKAVAD
                     KEDGDAA"
     misc_feature    complement(19165..19292)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            complement(19371..20060)
                     /gene="rplA"
                     /locus_tag="ACG842_000375"
     CDS             complement(19371..20060)
                     /gene="rplA"
                     /locus_tag="ACG842_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HFQ0246944.1"
                     /translation="MAKKSKKMQDALKKVDSSKVYPVAEAVALAKETNIAKFDATVEV
                     AYRLNVDPKKADQQIRGAVVLPNGTGKTQTVLVFAKGDKAKEAEAAGADFVGDDDMVQ
                     KIQNGWFDFDVVVATPDMMATVGRLGRVLGPKGLMPNPKTGTVTMDVTKAVNEVKAGK
                     VTYRVDKAGNIHVPIGKVSFDDEKLVENFAAIHDVLLKAKPSAAKGQYMKNVTITTTF
                     GPGIHVDQASF"
     gene            complement(20164..20586)
                     /gene="rplK"
                     /locus_tag="ACG842_000376"
     CDS             complement(20164..20586)
                     /gene="rplK"
                     /locus_tag="ACG842_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HFQ0246945.1"
                     /translation="MAKKVEKIVKLQIPAGKATPAPPVGPALGQAGINIMGFTKEFNA
                     RTADQAGLIIPVVISVYEDRSFTFVTKTPPAAVLLKKAAKIEKGSGEPNKNKVAKVSS
                     DQVREIAELKMEDLNAANVEAAMRMVEGTARSMGITVE"
     gene            20929..21597
                     /gene="sdaAB"
                     /locus_tag="ACG842_000377"
     CDS             20929..21597
                     /gene="sdaAB"
                     /locus_tag="ACG842_000377"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006565 - L-serine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent
                     subunit beta"
                     /protein_id="HFQ0246946.1"
                     /translation="MKDLRYRSVFDIIGPVMIGPSSSHTAGAARIGKIIRSIFGEQPD
                     SVDIYLYESFAKTYRGHGTDIALVGGLLGMEPDDERLADSLKLAYEAGMEVLFVPKSE
                     KAEHPNSVKMLLKKGTRKLSVTGISIGGGNIQISELNGFKISLSMGTPTLIVVHQDVP
                     GMIAKVTSILSETNTNIGTMTVTRESKGENAIMIIEVDDPQVEETVKRLKQLPNIDSV
                     NYFD"
     gene            21610..22482
                     /gene="sdaAA"
                     /locus_tag="ACG842_000378"
     CDS             21610..22482
                     /gene="sdaAA"
                     /locus_tag="ACG842_000378"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006565 - L-serine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HFQ0246947.1"
                     /translation="MFLSIEELVDQAKAYPSVAELMIAVEMEMSGRSRSHIIEIMEKN
                     LSVMEQSINEGKEGVTSVTGITGGDAKKLDDYLNEADFLSGETILKAVRNAVAVNEVN
                     AKMGLICATPTAGSAGVVPGVLMAVRDRLDLTHEQQLEFLFTAGAFGLVIANNASISG
                     AEGGCQAEVGSASAMAAAALVCAKNGTPHQAAQAVAITLKNMMGLICDPVAGLVEVPC
                     VKRNALGSSQAFISADMALAGIESVIPPDEVVAAMYQVGRQMPSIFKETAEGGLAVTP
                     TAKRLAEEILNKRA"
     gene            complement(22573..23673)
                     /locus_tag="ACG842_000379"
                     /pseudo
     CDS             complement(22573..23673)
                     /locus_tag="ACG842_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324221.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lactate oxidase"
     gene            complement(23765..24652)
                     /locus_tag="ACG842_000380"
     CDS             complement(23765..24652)
                     /locus_tag="ACG842_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFQ0246948.1"
                     /translation="MTEPIKNLCKRATAAALVLISLFALSGCQTTKINTTEVSDKEKT
                     AESSKVIEKTTIPTLFFHGYSGTENSFKGMLRRLEKNQQAVKELVLTVGTDGQIQATG
                     DLSGKADNPMVQVLFEDNKNNEWNQAEWIKTCLLYLKENYGIDKVNIVGHSMGGVSAL
                     RYLGTYGQDTSLPQVQTLTAIGAPFNDFVDDSAQSLTDELAKGPAVVSNRYQDYQQMI
                     GNIPITTRFFLIAGQLDETDLSDGTVPLNSALAVYALLKQRGNEIEEKVVTGENASHS
                     MLHENQEVDQLVSRFIWKE"
     gene            complement(24729..25274)
                     /gene="nusG"
                     /locus_tag="ACG842_000381"
     CDS             complement(24729..25274)
                     /gene="nusG"
                     /locus_tag="ACG842_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HFQ0246949.1"
                     /translation="MESFEKNWYVLHTYSGYENKVKANIESRAQSMKMADFIFRVVVP
                     EEKETEVKNGKEKEIVHKTFPGYVLVEMIMTDASWYVVRNTPGVTGFVGSHGAGSKPA
                     PLLPEEVNHILRSIGMSLRQNDLEVEVGEVVKIIEGAFAGLEGQVTEVDGEKEKLKVN
                     IDMFGRETSTELDFDQVDKID"
     gene            complement(25375..25545)
                     /gene="secE"
                     /locus_tag="ACG842_000382"
     CDS             complement(25375..25545)
                     /gene="secE"
                     /locus_tag="ACG842_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HFQ0246950.1"
                     /translation="MKFLKSVKDEIKQVTWPSRKQLRKDTLVVIETSIIFGLMFYVMD
                     TAIQSLFGWILK"
     gene            complement(25569..25721)
                     /gene="rpmG"
                     /locus_tag="ACG842_000383"
     CDS             complement(25569..25721)
                     /gene="rpmG"
                     /locus_tag="ACG842_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HFQ0246951.1"
                     /translation="MAKKKAALACSVCGSRNYTKSVSEGKSGERLEINKFCKYCNQYT
                     LHKETK"
     gene            25883..26527
                     /gene="cbpA"
                     /locus_tag="ACG842_000384"
     CDS             25883..26527
                     /gene="cbpA"
                     /locus_tag="ACG842_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-AMP binding protein CbpA"
                     /protein_id="HFQ0246952.1"
                     /translation="MLLKTLVYKKKDLTTVTETSTLEEALKILETSGYRCVPILDSSG
                     NIFRGNIYKMHIYRHKANGGDMTLPVTHLLKNATKFIYLDTSFFKVFFTIKELPYIAV
                     LDSENHFYGILTHSTLLNMLSQSWSVEQGSYVLTIASTGKQGDLAAIAKIISKHSSIA
                     SCITLDIEKDEYIRRTLITLPANTEAKVCETIVNHLEKKNYKVVEIENLQNQEA"
     gene            complement(26583..27086)
                     /locus_tag="ACG842_000385"
     CDS             complement(26583..27086)
                     /locus_tag="ACG842_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HFQ0246953.1"
                     /translation="MQKTKSRQRLSVIVVNGIIMALYLALTILVAPVSSGPIQFRISE
                     SLNHLVVFNRKLLWGVLGGVIVYNAFFGFGIMDVVFGGGQTLLSLGLTALLQNKVKNV
                     KMRLVLNTIFFTISMGLIAYMLVPTGGQAFWTTYGTLALSEFIVMAVSAPLMYYLDRA
                     LDFEKRV"
     regulatory      complement(27099..27145)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            27683..28648
                     /locus_tag="ACG842_000386"
     CDS             27683..28648
                     /locus_tag="ACG842_000386"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HFQ0246954.1"
                     /translation="MKFHYHILINPSAGSGNGYKVAERILPVLKNKHIDYTLHYSEYK
                     GHDAEIAETLAKETLIPWIEEEQKTIDTFPLLIIVGGDGTLHQVLDTFYQMEVEFPVA
                     YIPAGSGDDFARGAGLPKNPKKGLQLILAAQSPEKVHIMAYEEKISEKKGIAVNNFGI
                     GLDAAIVHATNHSSTKKRLNKYNLGSFSYLFSILRALFTQKGFPILVDAGGKQLSFSN
                     AFLCTATKHPFFGGGIAIVPTADASKPVIDFVVVERINFFKILWLILLLIQKKQLKSK
                     YFHHYTTNRLRIVSTTPQHGQEDGEEMEKRPFDITITTTKQLFWC"
     gene            complement(28747..29919)
                     /locus_tag="ACG842_000387"
     CDS             complement(28747..29919)
                     /locus_tag="ACG842_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0246955.1"
                     /translation="MTLYKTIWTLLKANKSNLLIGVIITVVATFFFAQNIDVENTSLQ
                     DAKIALISEEESPVVDGLRKYLNDKETVVTLDGTSQKEIDDALYFNRVNIILHLPADF
                     TEQVEVGKMPTIKIQSRPDAFSKTVVTQQVNTFLQTLILFQEEDKTIDAAMEQTQMAL
                     SVTGEVELSAGYSQRMKKLLTGTTFNFLSYGLFLSIFSGFSVINLAFNRKEISKRNQS
                     APITKRNLNRKVTFSLISYSLLLFSGFLLLMVLLVIHTSWDITVWYHILNTFLFLLPI
                     ISFSACITSLVKNSETSGGIKDIFITGSCFIGGVFVPAEYLPEMVSKIAAFTPTYWFV
                     QNNNLISETLSYNQTFAESFWFNGCVLVAFAAVFSMIQFILGKERNYRWQPNWAKN"
     gene            complement(29906..31051)
                     /locus_tag="ACG842_000388"
     CDS             complement(29906..31051)
                     /locus_tag="ACG842_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0246956.1"
                     /translation="MFWHFYKYRTKILLRNKSMLFWTLAFPILLGLMFMAAFGEIDQG
                     GNLETINTGIVIKADQTALSENFTTVLEQVEVNDKPLFEVQELSEVEANEKLNADDLA
                     GFYLIDGSSVSLHVGQAGMSQTLMKNVMDQFLQRIGIISSKVAEVENVDLAPIMTVFE
                     QEVSFQEVHAGRNMSFKSFYFFTLIGMAILYGTMWGVRNVQDQQANQSSNGIRLSLIP
                     RKQSLVSIANLAASFTIVLTEIYIMLAVFRFIYQVDFGERWQWLMIVTALGSLCAILL
                     GTLVGNLIPKMNLVQKDSILISVTIAMSFFAGMMGSEQVKYWIDLHIPIVGQLNLVNL
                     ISESLYKLYFYTDLASFYTNLLWLIGFIFLFALGNAWMERGVRYDAL"
     gene            complement(31065..31989)
                     /locus_tag="ACG842_000389"
                     /pseudo
     CDS             complement(31065..31989)
                     /locus_tag="ACG842_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509966.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            32106..33185
                     /locus_tag="ACG842_000390"
     CDS             32106..33185
                     /locus_tag="ACG842_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HFQ0246957.1"
                     /translation="MHKSRLLNETSFLLVISLLLLVSQGFVAMQVIYLLIAVLFTGVS
                     YLLPTKPNWIFQVILFLLCAFLPDLVLFLPATMRVIAPEESHLDYLFLLGFLLISTVG
                     NHPPIKVAFVVILLFFSFYLRKKDQRTTDLEKKYIVSKDTHWEKQQELSEQNKALALS
                     QETQLALQIVEERNRIARDIHDNVGHLLSSAILQIGALEMINQEPKLKEPLENLSATV
                     HTGMDRIRQSVHDLHETSISFQHSLEFLIADFPSSVTIEGNLFEGLNETQQNCFLMVI
                     KEALTNSIKHSHAKTVVLHFRTLPAFYRLQITNDGTSPQKNSAPGIGLKTMRQRVQEQ
                     KGQVHIFQENQQFQLTIILPREGHQ"
     gene            33182..33810
                     /locus_tag="ACG842_000391"
                     /pseudo
     CDS             33182..33810
                     /locus_tag="ACG842_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325326.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
     gene            complement(33920..34147)
                     /locus_tag="ACG842_000392"
     CDS             complement(33920..34147)
                     /locus_tag="ACG842_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFQ0246958.1"
                     /translation="MAKETEDKLAKLAKESGNDFKKLDEMEKEFKLNESPDVERDDRE
                     ESKLEKTLDKAPIFPPNTPNNPTNPGSPANP"
     gene            complement(34250..36838)
                     /locus_tag="ACG842_000393"
     CDS             complement(34250..36838)
                     /locus_tag="ACG842_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0043139 - 5'-3' DNA helicase activity
                     [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HFQ0246959.1"
                     /translation="MQPEEMPYFVGTVAAIFFQNPSNFYKVLLVHISETTADYREKEI
                     VVTGSFGDIQENETYRFYGELVDHPKYGRQLKLERYEQEKPTTANGIVAYLSSEKFPG
                     IGKKTAEKIVELLGEDAIEKIIADPSVLKQISGLTKPKREMLAETIRLNHGMDQVIMG
                     LNRYGFGSQLAFSIYQAYKNESLDIIEENPYQLVEDIEGIGFKRADNLAEQIGIAADS
                     PRRIRAAILHQIFQHAVQTGDTYVPAETLLQQTIRILETSRPIEIQPDQVATVIIQLV
                     EEGKIQQEETNLYENSLYFSEWGIGNSIQRLLSRKKEIHYPEKEVQKNIRRIEKIFGI
                     QYGPSQEQAIAEAIRSPLFILTGGPGTGKTTVINGIVNLFAELNGLELDPSKYTQEIF
                     PILLAAPTGRAAKRMNETTGLPASTIHRLLGLNGREKTPSVSTKELEGGLLIVDEMSM
                     VDTWLANTLFKAIPTNMQVILVGDKDQLPSVGPGQVLHDLLEIEQIPKMELNQIYRQG
                     DGSSIIPLAHEIKEGRLPKDFTKNQKDRSYFRSDAYQIEPLISKIVEKAKNKGFRAQD
                     IQVLAPMYRGPAGIDALNKMMQEIFNPGKPGKKEVHWNDTVYRIGDKVLQLVNTPELN
                     VFNGDMGEITGIILAKDSEDKVDELVIQFDANEVTYKRNEWNKITLSYCCSIHKSQGS
                     EFKMVILPMVHQYQRMLQRNLLYTAVTRSKELLILLGEEQAYVTCVANESASRMTTLK
                     SRIMDNDKMNITLRTKLAAYEDSLVGNDPFEDGNYTKEPRVVKEKLPVEDDSGTNETK
                     NQVMETVEPDLFSSFVGEEKKEEVEPAVSKDFVLTAEVIDNQLVDPMIGMENSSPYDF
                     IPAKNA"
     gene            complement(36858..37517)
                     /locus_tag="ACG842_000394"
     CDS             complement(36858..37517)
                     /locus_tag="ACG842_000394"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HFQ0246960.1"
                     /translation="MELYFTRHGKTQWNQERRFQGSNGDSPLLPQSYEEIKAFGKKVK
                     YVPFKAIYSSTAKRARDTAEGINKELARPVEIFYTDKLRELGLGTLEGQSIDEMYQKY
                     PDNLPNLRNHLDLYDPSPFHGETIESAITRIETVVADAVAQHKDGPLLFVGHGASLTA
                     AIQWMTGKELSELREMGGLFNSSLTILETEEPCRLLPYELKVWNQTDFLGTETAPEPL
                     L"
     gene            complement(37566..38069)
                     /gene="trmL"
                     /locus_tag="ACG842_000395"
     CDS             complement(37566..38069)
                     /gene="trmL"
                     /locus_tag="ACG842_000395"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008175 - tRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HFQ0246961.1"
                     /translation="MNHIVLFEPLIPANTGNIARTCAATNTPLHLIEPLGFSTDDKHL
                     KRAGLDYWNDVNITYHSNLEAFLTFLGNRKLHLISKFAHKVYSDENFADGEEHFFLFG
                     KETTGLPETFMRENEEKCLRIPMNDEHVRSLNLSNTAAMIVYEALRQQGFPNLELTHH
                     YENDKLD"
     gene            complement(38290..39156)
                     /locus_tag="ACG842_000396"
     CDS             complement(38290..39156)
                     /locus_tag="ACG842_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HFQ0246962.1"
                     /translation="MLKKIDRGRQFLAENHQLFRCPVCQEEVKKSDNGLICKNNHRFD
                     LSKKGTLYFLSHAVKTEYDQGMFVPRRRMIQSGMYQPVIETIAQHLPEEVSLLDVGCG
                     EGSFLNELSKLKKIQNAVGFDISKEGVYAATEQSAEAFWCVADLTNLPFADQTFNAVL
                     NIFSPSHYQEFQRVLSEDGEVLKVVPQENYLKELRQAFYPNQPEKQSYSNERVVAKFA
                     EAMKLTERQRVTYEFEIPEENQKDLLAMSPLEWQVEERIKADLYKNPLKKITIDIELL
                     KGTKRKRFSSSF"
     gene            39265..39906
                     /locus_tag="ACG842_000397"
     CDS             39265..39906
                     /locus_tag="ACG842_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0055070 - copper ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HFQ0246963.1"
                     /translation="MIKEFCAENYTSIPLAIARGADRIELCDNLAVGGTTPSTGVIEE
                     TLSYAGEKDIPVMTIIRPRGGDFVYNDIELKIMHTDLIEAKKLGTDGVVFGCLTPSGW
                     LDEEALELLIETAEGLQITFHMAFDSIPEDRQFEAIDWLVEHGVDRILTHGGPADKTI
                     VENFPRLKELIAYTDGRLIILPGGGVTAENAAHVAKELNVSEVHGTKIVDLQL"
     gene            complement(39968..41329)
                     /gene="mgtE"
                     /locus_tag="ACG842_000398"
     CDS             complement(39968..41329)
                     /gene="mgtE"
                     /locus_tag="ACG842_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HFQ0246964.1"
                     /translation="MDENQELEERFAQLTQDLRENNIQAFRNSFLEMHIYEQGQFYQS
                     LTEENRQLIYSYLSPKELADMFDVIEEDNEHMKEYLDEMRPSYAADMLSEMYTDNAVD
                     LLNTLDKKQIAKYLSLMSADDASEIKELLHYEDETAGAIMTTEFVSIVANQTVRSAMY
                     VLKNEADVAETIYYIYVVNQEGQLVGVISLRDLIVNDDDAMISDLMSERVLSVHVGDD
                     QEDVAQTFRDYDFLALPVTDYDDHLLGIVTVDDIIDVIDDEAASDYSGLAGVNVEEIN
                     ENPVKAASRRLPWLITLLFLGMSTASLISHYEDLVSEASILAVFISLITGTAGNAGTQ
                     SLAVAVRRLAISDEKDHGFVRLVLAEVLTGVVTGAVTGITIFLIVGFWKHNFILGFVI
                     GMAMLFAITVANLAGSLIPMLMDRLGFDPAVASGPFITTLSDLTSVLIYFNIASLFMG
                     YFM"
     gene            complement(41347..42246)
                     /locus_tag="ACG842_000399"
     CDS             complement(41347..42246)
                     /locus_tag="ACG842_000399"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HFQ0246965.1"
                     /translation="MQFTWDYEKDHSQQVKYFLKEKGISKGLLAKIKFQGGQIKVNDQ
                     VENVLFSLAKDDKVTIVIPAEGEHETVLLDETPIDIVYEDEHVLVVNKPAGVSSIPAQ
                     YHPNGTMANRVKAYYKKQGYENQVIHVVTRLDRDTSGLMLFAKHGFAHAKLDMQLREK
                     KFVKKYQALVSGRLSDLSAHGHIDLPIARDYSSLLKRMVSDEGRSAQTEYWIEKRLTE
                     IALVDIQLHTGRTHQIRVHFSAIGCALLGDDMYGGRMDLGIKRQALHCYDLRFYHPFT
                     EELLEFKQPLAADMEAVVRRFEE"
     gene            complement(42248..43045)
                     /locus_tag="ACG842_000400"
     CDS             complement(42248..43045)
                     /locus_tag="ACG842_000400"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006741 - NADP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HFQ0246966.1"
                     /translation="MKVAIVHNKEAKTIEVTERLNALLEQAGIQRDDHEPELVISVGG
                     DGTLLSAFHHYSHCLNDVRFLGVHTGHLGFYTDWRDYELEELVDSLRTNREQSVSYPL
                     LDVRISYLDETPDQHFLALNESTIKRANRTMVADVYIKNELFESFRGDGLTISTPTGS
                     TAYNKSVGGAVIHPSINAFQLAEIASLNNRVFRTLGSPIVIAHDEWLEIKLENTEDYL
                     VTVDQLNVAKANIRSIYYRIAEERIHFASYRHMHFWHRVKDAFISED"
     gene            complement(43023..43727)
                     /locus_tag="ACG842_000401"
     CDS             complement(43023..43727)
                     /locus_tag="ACG842_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HFQ0246967.1"
                     /translation="MERDWEAFLAPYEQTVSELKVKLRGIRKQFREQNRHVPIEFVTG
                     RVKPVDSIVTKSQLRHIPMGRLEEEMSDIAGLRIMCQFVEDIHQVVEIIRKRKDMKVL
                     QERDYITNKKASGYRSYHLVIEYPVQLISGEKKILAEIQIRTLAMNFWATIEHSLNYK
                     YQGVFPEEMSERLQRAAEAAYQLDEEMSSIREEIQEAQRLFSHGRGKSDDVYYRTLLK
                     KESEREEAGDESSDRS"
     gene            43858..44434
                     /locus_tag="ACG842_000402"
                     /pseudo
     CDS             43858..44434
                     /locus_tag="ACG842_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373095.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
     gene            44552..45229
                     /locus_tag="ACG842_000403"
     CDS             44552..45229
                     /locus_tag="ACG842_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP adapter SpxH family protein"
                     /protein_id="HFQ0246968.1"
                     /translation="MIEIYLFVNPLGEYCFEMEQQLLQFIEEEYGTPSKEKMQFRFLP
                     LVNLQTIGDVMQQKGISQNNLEERNHLFSMTYSAALDCKAAQFQGKKKGRRFLIKLQE
                     AVGCNDIPYSQELAESIFEEIGGDLNMFKEDRQSDFVKEVFFSDQKIAREMGITKHPS
                     AVVYNYACDRDFGVLVEVKGTSSLWRITELCKTTDNSYQVFNGEHLHTNDSQFHSRTS
                     HLHLLSN"
     gene            complement(45308..47116)
                     /gene="pepF"
                     /locus_tag="ACG842_000404"
     CDS             complement(45308..47116)
                     /gene="pepF"
                     /locus_tag="ACG842_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HFQ0246969.1"
                     /translation="MEVKQLPKREELPENLTWDLTKIFSSDQEFDEKYLELSEELKQS
                     EKHKGTLDQGASQFLNAIEFVLRVYRQTEVIYVYAHLKNDQDTGNTDYQALYARASSL
                     FSKVSEAVSWFEPEILQLSDDQIWQYFKEEPKLEVYRHYIQQIVDNRAHVLSAEQESL
                     LAGAGEIFDASSDTFAVLNNADLVFPTIEGENGEIVQLSHGVYGQLLESTDRRVREAA
                     FKGLYSVYEQFRNTFASTLGTHIKGHNFKAKVRNYSSAREASLSNNHIPESVYDTLVD
                     VVNKHLPLLHRYMELRKRLLEVEKLHMYDLYTPVLGEAPITFTYEEAKEKALEALKPM
                     GEEYMAIVEKAFSERWIDVVENKGKRSGAYSSGSYDTNPYILLNWHDTLDQLFTLVHE
                     MGHSVHSYFTRSNQPYVYGDYSIFLAEIASTTNENILTEYLLETEKDPRVRAYVLNHY
                     LDGFKGTVFRQTQFAEFEHFMHTEDEKGVPLTSEYLSDSYGKLNAKYYGPAVEEDPEI
                     KFEWSRIPHFYYNYYVFQYSTGFSAASALAKKILNQEPEALENYLAYLKAGNSDYPVE
                     VMKKAGVDMTQAAYIEDAMSMFEQRLNELEELIDRL"
     gene            complement(47292..48290)
                     /locus_tag="ACG842_000405"
     CDS             complement(47292..48290)
                     /locus_tag="ACG842_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA family protein"
                     /protein_id="HFQ0246970.1"
                     /translation="MLLAMNEYNKCCFAKHAEKGKKYYCRGCRKRVILKKGKKKCAHF
                     AHQKSDNCSVFSERESKEHLQLKECFMDWLGQSVEPVFLEAYLPRLRQRPDILLANLA
                     IEIQCSRLSHQRFIERTQNYLNNSYQVWWILGNSFLGQSQFSLIEKSCCYYNRKRGVH
                     CWKADLKKQKLYLYHHITETVSGHISFCSSCWTFSSRDLKEIFTSNEIKMNQMKKIEK
                     VSEDGKKWLTRQLIHKQKNTVSIQEQCYLRHKHLLHLSSWIYQKSRFFFYLQEQVFLY
                     RMLYEEALENQKVPDFNSWLCQIKEHKREWLFPMIDEEMVYQQFFNECIHLSSLKK"
     gene            complement(48516..49169)
                     /locus_tag="ACG842_000406"
     CDS             complement(48516..49169)
                     /locus_tag="ACG842_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HFQ0246971.1"
                     /translation="MEMEHINENTIRVLIGHEDLEERGVSFLDLLGNHKEIENFFYSI
                     LEEVDIEEEFRGSEAVTFQVLPKGDGLELFISKNLPDEEISQSEDSTVDSEDVSDFLR
                     QQIIGSDPEEELSGSALEEQERQATFAFEDFEQVIQLATDVELESAWTDLYLFEERYY
                     LVVHFWMENLNQADVENQIARILEFAEKSDRTPETLNEYGQCLMARNAIERTKFYFV"
     gene            49603..>50742
                     /locus_tag="ACG842_000407"
     CDS             49603..>50742
                     /locus_tag="ACG842_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086557923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HFQ0246972.1"
                     /translation="MSHTQLIKDTLNILDLNIYFEENCLTKEKYKGQICLIYKGTLLY
                     KPEECSHCLCVVPSRIIRWGTTTVRLLLNDVSEYRTYLELKKQRFKCKSCQRTFVADT
                     SVAEKHCFISQKVRWSVIARLKENTSMTEIARQKNISTSSVYRVMKRFYRPLNPFKQT
                     LPKVLCFDEFKSVRSVASAMSFIMMDGQSHALLDIVENRRLPYLERYFSRFSLATREA
                     VQLIVIDMYAPYVSLVKKLFPNAQLIIDRFHIVQHIGRTFLNHRVKETTVRLKGNSHS
                     QRGLGKKLKRYWKLLQKEEAKLNYEKRVWRASFKDYLTESEIADRLLAACPNLRQGYQ
                     LYQDILYAVKKRDQSLFEDCLTRDISGLPETYTTTLRTFKKFLPQI"
BASE COUNT        15178 a        10641 c         8969 g        15954 t
ORIGIN      
        1 ctatgtatat tctttaatat tttttgagaa tgcacttcgt acataataaa ctttaatcac
       61 attagtcttt catacctacg atcgatctct ttgactcgca cccataaatt cataatagtt
      121 tgtcggcgtc tttggacgtt tcgaagatga gctacgcatt ctcttttttt atagacaaaa
      181 aagatagaga cgaacatgga gcttccaaat ttggatattt gatccaattt tggaagctcc
      241 atttatttcg tgtctgtctt tttatttttt ccagctttcg actaattctt ggttctcttt
      301 gatccagtct tttgctgctt gctgtggatc ttttccattg ttgatttcca acataacttt
      361 ttccatatct ttttctgacc aatggaaatt gtctaatact ttgtaagctt ctggctggtc
      421 ttctttcaat ccttttcgaa ccatcgtatg gatctgctct tcgcttccca ttccgttttc
      481 aggatcttct aagtatttca gatcatattt ggcaaacatc caatgaggtg tccatcctgt
      541 aactacaatt ggttcgtgtt ttttgatcgc ttgacctagt gcaacggtca ttgctcctga
      601 agaagatgtc tctactttcc agtctttcaa gttgtcatat ttttggatcg tgttttcagc
      661 agccgtaact acaccagctc ctggctcgat tcctatgatt ttcttacctg cctgatcggt
      721 taactcatcg atcgaattta catccatgta ggctggcact gctaaaccaa cttttgctcc
      781 tgtcaaattg gcacctaggt cttcgacttg atctttatat tgtgcatatt gtgaaccatg
      841 agttttaggt agccaagctg aaaccatggc atctgactcg ccatttgaaa cagatttcca
      901 catgattgaa ttatctaatg gcgtcatcgt tacatcatag cccatttgtt tcaatacttc
      961 accgactaca tttgttgaag caacttctgt atcccattca acataagata agttgatacg
     1021 ttttgtttct ttggcagatg aaaaactccc tataccaatc gcacctaaga tcactactac
     1081 tacagcacca atgatcagtc cttgacgttt tttacttttc tgtgttgttg ctcctgcagc
     1141 ttttttattg tttggctggt tcaaatgctg tgtaaaacgg tcaatgatga ttgctaagat
     1201 aactaatgca accccgttga caaatccatt cccgacttgt gctctttgca atgctgataa
     1261 tacgccgcgt cctaagccgg gtgcaccgat catagaggca ataacgacca ttgaaagtga
     1321 cagcattgtt gtttgattga tccctgccat gatcgtactt ttagctaaag gcaattctag
     1381 tttgaatagt ttttgacgac ttgtgctccc gaatgaatca gaagcttcta ctaattcttt
     1441 aggaacttgt cgaatcccta aattcgtaaa acgaacggtt ggtggcagag caaagatgac
     1501 tgaggcaaat acccctggaa ccatcccgat accgaaaaat gcaactgctg gaatcaaata
     1561 aacgaatcct ggcattgtct gcataaagtc aaggatcggt ttgataatac tttgggcttt
     1621 actgcttttt gccatcaaga tccctaatgg taccccaatg atgatcgaga tcacactaga
     1681 taaaagaaca agtgtgacag tattcatcaa atcattccat agaccttggt tataaataaa
     1741 ccatagaccg attaatgtga acaacgtaag tcctggacgt ttcttagaaa taaagaaagc
     1801 tgctaccgtc aataacaaga taaacaaagg agctggaatc actaacagta aatttgtaat
     1861 ccccgacatc actgcctgtc caatcgtttg taaaaatgaa aataggccag aaaaagttgt
     1921 cgtaaaccat tctgtgatat tttctaccca gtttgccact ggtaattgat agttcaatag
     1981 attattcatg ttcactcacc tcgcttgttt ctgctaatgc ttcaatcaca ctacctcgga
     2041 tgacgacacc ttttaatttt ccatcctcta caactgcaag tggagtcggt gagtcataaa
     2101 taatattgaa aatatcactt accaccatgt ctttcccaat caccgtcaca ttttcatcta
     2161 ggaagtcgat caatggacgt ttttgcttcc tagcttctaa cgcttgatct gcagtgatga
     2221 tccctttcag gtgtctcttt ctgtcaacag ccatcagcat gctgacttct tcattacgca
     2281 tccttgtcaa tgcaacgttt ggtccatcag attcgatatt cgtcgtcaat gcaggtacca
     2341 tgatgttttg agccgtaagc actttggaac gatcgacttc ttccacaaat tcacgtacat
     2401 aatcatttgc tggattggtc aatatttctt cacctgttcc gatctgcatg atttcgccgt
     2461 ctttcatcaa ggcgattctg tccccaatcc gtaaagcttc attcaaatca tgggtaatga
     2521 aaataatcgt tttttgaaca ttggcttgca ggtccagtaa ttcgtcttgc atttctcgcc
     2581 ggatcagcgg atcaagagcc gagaacgctt cgtccatcag caagatctca ggatcatttg
     2641 ctaaagctct ggctaggcca acccgttgtt gcatcccacc agataactga ctaggatatt
     2701 ggtctttaaa tgaaagcaaa ctagaatttt ctaatgcttt ttctgctttt tcttgtcgtt
     2761 cttctttagg tacaccacgg acttctaacc cgtattctgt attttccaag atcgttcgat
     2821 gtggaaacag accgaagttt tggaaaacca tattgatttt atgacgacgg acttctcgca
     2881 attcttcttt gcttaacttc gatacatctt ctccatcaat atagatactg cccgaagttg
     2941 gctcaatcag acgattcaat agacggatca atgtggattt cccactcccg gaaagcccca
     3001 tgatcacgaa gatttctcct tcttttacgt caaaattcac gtcatacaca ccaaccgttg
     3061 caccggtttt ctttaaaatc tccgttttcg gctggtgatc attcatcata tctaaagcgg
     3121 cttgtgtctt tttaccaaat atctttgtta aatgttttac ttctactttt accaaacaaa
     3181 ttctcctttt ttatgtaaat tcatttttct ttctgaaaaa agtgaccaca ctatgttaac
     3241 atcatgtagt cactttgtca aaaatgaaag aaatttgctt gtccaaactg aaaaaaactt
     3301 ggctaaatct ttgtaaataa aaggtttagc caagttaata tttttttaat atttcacaca
     3361 atcattctgc aagctgatgc tccgggtaaa aaaagtttgt catacgctgt agtgtatatt
     3421 catttccaat aaaatgaacg atgtcccctg ccgtaaattt agcatagggg ccaggtgaaa
     3481 caagcagttc ttcattatga gaaattgcga taactgtggc gccagtcgcc tgccagatat
     3541 tcaactcgct aattgaacgg tccagatgca ttgctttttc tgataaagat aattcaaacg
     3601 gaagtaaagg attcatctta tgtattcgct tcgtttgatt gaccagctga tccaataatt
     3661 gagaaaaatg attcagctca tcttgttgct tctccacact tgccatgatt tctgcttttg
     3721 tatcttggat ggattgtaca tcttgatatt gcaataggaa atcttttgct ttttccttcg
     3781 aagcaacaaa tgccccactt ccatgccgta cttccatgat tccaagatct accagcacat
     3841 tgatagcctt gcgggtagtc tcaggagaag cattgtacgt actggcaagt gttgagcggg
     3901 catggatctt ctcacccact ttatatcggt tttcaacgat acgttcggct aaatcaacag
     3961 caatctgctg gtagcgcggg cggacgattt ttttctttcc agacattttc tttcctcttt
     4021 ctttttttac tttccctcat cttactatgc tctagcaaaa taagcaaaaa aggggagaat
     4081 agcctcttgc aacgactgat catcgcttga tctgttcctt ttttactggg caagctgttt
     4141 gacagtcatc acaattctta ccggatttta aacgagtata gacaattgcg cctgcacttc
     4201 caaagattaa cacacttaat aagaatgtcg ccatcctatt cctctccttc taatgacaat
     4261 atagtataaa actggttctt ggattttggc tgacggaaca aaacataaac gagtcctatc
     4321 atcagtaaaa tagcaagata cgtccctaac gctacctgtc caccctccaa taagtgtccc
     4381 aactgataaa tcacaaaact aactaaataa gctaaaccgc attgatagcc tatggcgatc
     4441 caagtccatt tcttggcatt catttcccga tggatagccc caattgccgc aaaacaaggc
     4501 gcacaaagta aattgaaggc taacaatgaa taagctgctg cgggagtata tgtgtgttgt
     4561 agcacttgcc agatttctct gccattttcg gatacttccg catgaccaaa caaaatacca
     4621 aatgtgccaa taatattttc tttagcgacc aatcctgtaa tcgctgcgac tgttccttgc
     4681 cagtttcccc aaccaagcgg ctgaaaaaca actgccagga ttcgcccgaa aaaggctaag
     4741 atactttggt cttcagaaac aggttgagca taaaaattat aagaagagct gaaccagatc
     4801 acgatactag aaacaaagat aatcgttcct gcttttttaa caaaagactt gctgcggtca
     4861 taggtttgtc ttaggaccgc tgaccattga ggaaaatgat atgcgggcaa ttccatgata
     4921 aaaggagctg tatctccagc aaacaaacgt gtttttttca agctgattcc agagaaaata
     4981 attgcagtca taccaagaaa ataggcagaa ggagctaccc aagattgatg aggaaaaaag
     5041 gcccctgcta ctaaggcaat aatcggcaac tttgcagaac aaggcatgaa ggttgtgacc
     5101 atgatcgtca aacgacgatc ttgctcgttt tcaatcgtgc gactggccat gacgcctggc
     5161 actccacatc ctgtagcaat cagcatagga atgaaggatt ttcctgaaag accgaatttc
     5221 ctgaaaagtc gatccatgac aaaagcgatc ctcgccatat atccgcagtc ttccagaaag
     5281 ctcaagcata aaaacaatat aatcaattgc ggtagaaatc ctaacactgc tcccacgcct
     5341 gcgatgatgc cgttcaagat cagctcctgc atccatcccg ctacttgcca ttcagctaac
     5401 aatctgccaa catgttctgg taaccatgta ccaaacaatg tgtcattgat ccaatctgtc
     5461 cccattgttc ctactgtttg aatagccaga taatagatca gccacatcac aaatgcgaag
     5521 atcggcaagg ctaaccaacg attcgttgca atctgatcga ttttgtcact catcgataat
     5581 tggaaagaat catttgacac aacacagagt gtacatagtt gggtaataaa ttcataacgt
     5641 tcattgacca gaatcgtctc actatcgtcg tccaatagtt tctctgttaa ctggatcagt
     5701 tgctcgatct ctttttgctg agaaactgct atctcgtaat cctctcttac cttctgatct
     5761 cgttcaaaat atttcattgc ataccagcgt ttcttgccgc tttgttctcc tagtacatct
     5821 tcaatttcat gtaaagctgc ttctaaacgt ggatcatatg tacagggaac aggaatagaa
     5881 ggccgctttt ttgcagactc ctgaatcttt tcgattagtt tttcgattcc ttgctttttc
     5941 aatacgctga ttggtaaaac tggtacacca agaccatagg aaagcttttc tagattgatt
     6001 ttttttcctt cttttttgat gacatccatc atattcagac caatgatcat aggaatgtct
     6061 aaatccaata actgcgtagt taaataaaga tttcgttcta aattggtagc atcaataatg
     6121 ttgatgatca gttcaggctg ttcctgaacc aaataatctc ttgcaatcac ttcttctggt
     6181 gtataaggag acaaagaata gatgccgggt aggtcctgaa tatgaatgtt cggatttttt
     6241 ttcaacgatc cttctttttt ttctactgtt acgcccggcc aattccctac atattgattc
     6301 gaaccagtca agagattgaa caagctagtt ttcccgctgt ttggatttcc gactaacgcg
     6361 atattcattc tttttcctcc ctttgtatca agattagttc ggcctctgac tttcgtaacg
     6421 tcagttcata ccctcgcact gtcagttcga ttggatctcc cagaggggct agttttcgga
     6481 taactatttt tacatttcgc gtcaatccca tatccattaa ccgtcgcttc actgcgcctt
     6541 gtccgtggat tgctacgacg atcccagtct ctcctgcttt taattgatca agagaagtcc
     6601 agctttcaga ggaagcagtc tttttattga caaagatttg acctagtatt tcccgactta
     6661 ctgccactcg atgacttttt accagtaaaa tggcattgtt gcctgataaa ttgacgacga
     6721 cgattttact tccggcaacg atcccgatat tttgcaaatg gcgttttgtt ttttcttctg
     6781 tttcgattcg ttcgacttga taaacgaaat tctgctttgc ctgcgctaag gtctgcatat
     6841 tgattcctct cttgtgaaat aaatcatgta ttaaaatcat tcattatatg ttctaatttt
     6901 cttacgtgtt acgcaaataa atgagaatca ttctcacata gaatactctc ggtaattgag
     6961 aatgtcaatt atacttgtga tattatgagc gaatctttag tattgataga taaaaatgag
     7021 aaaaaaatat ttcttcatag ttttccacaa ttttttctct attaaatttg ctattttccc
     7081 acacgatact tgttttcagc tcttttttca aaaacacaaa acttagtatt ccacagtttc
     7141 cacaacacaa tatgtagtgt ttgttgtttg acttttttac taagagtttt atactatcta
     7201 ttatagataa taagaatgtg ttagaggagg cactcaccat gaacgtaaaa atcttttcta
     7261 aaaataattg tatgcaatgt aagatggtca aacggttttt aacagaaaat caaattgctt
     7321 ttgaagaagt caatatcgat gaacagcctg aagcagtaga ctggttgaaa gcacaaggat
     7381 tccaaagtgt tcctgtcatt acttctgatg cagaaacagt tatcggattc cgtcctgatc
     7441 agttgcgtaa acttgccagc taacatgact aaaagaatca aaacgtgata catggatttg
     7501 aagggcttga gacagcacca gtttttgtcg caaacttcca aatccttttt taaagcttaa
     7561 aaacaatgca atgatttgca atcaagcatc atcaaatacg aaatgaaggt tgggcgtacg
     7621 tatgagtctt aaaaatttac aagatgtcag ttatttcaag ctgaataatg aaatcaatcg
     7681 gccagtcaat ggtcaaatcc ctttgaataa ggataaagaa gctttagttg ccttttttga
     7741 ggaaaatgtc aaaccaaata cgatgacttt tctatctatt atggaaaaaa tcaattactt
     7801 gatcgatcaa gaatatatag aagcagaatt cattcaattg tattcaccat catttattga
     7861 aaagctatat caatttttgg acgagcaaca tttttctttc aaatctttta tggctgctta
     7921 caaattttat tctcaatatg cgttgaaaac caacgacggt tccgcttatc tggaaactta
     7981 tgaagatcga gtagccttca atgcgcttta ctttgcgaac ggggatgaag aattagcact
     8041 tgctcttgca gacgaaatga tccatcaacg atatcagcca gctacacctt ctttcttgaa
     8101 tgctggacgt aaacgtcggg gcgaattggt gtcttgtttc ttgactcaag tcactgacga
     8161 tatgaactct atcgggcgct caatcaactc tgctttgcaa ttgtcccgta ttggtggcgg
     8221 cgtagggatt actttatcaa atcttcgtga agccggcgct cctatcaaag gatatgaggg
     8281 tgctgcaagt ggtgtcgttc cagtcatgaa attattcgaa gatagtttca gctattctaa
     8341 tcaattaggt cagcgtcaag gagctggcgt ggtttatttg aacgttttcc atccagacat
     8401 tattgctttc ctttcagcaa aaaaagaaaa tgcagatgaa aagattcgcg taaaaactct
     8461 ttctcttggc gtgatcgtac ctgataaatt ttatgaattg acacgtaaaa acgaagatat
     8521 gtatttattt agtccatata gtgtcgaaaa agaatatggt gtcccattct cttatgtaaa
     8581 tattacagct gagtatgatc atctagttgc aaatccaaac attcgcaaac aaaaaatcaa
     8641 agcgcgtgat cttgaaaatg aaatctcaaa attacaacaa gaatctggct atccttacgt
     8701 aatcaatatt gatacagcga accgcgagaa tccaatcgat ggcaagatca tcatgagtaa
     8761 tttatgttca gagattttac aagttcaaac gccttctgtg attaacggca aacaggaata
     8821 tgaagtatta ggaacggata tctcatgtaa cttaggctcg acaaatatcg tgaatttgat
     8881 gcaaagccct aactttggtc aatctgtgcg tacgatgact cgtgcattga catttattac
     8941 agatgcatct gatatcgatg tcgtaccacc gatccaaaac ggaaataagc tgaatcatac
     9001 gattggatta ggtgccatgg gcttacacac ttatcttgca aaagaacaaa tggaatatgg
     9061 ctcagaggat tcattagact ttacaaatat ttacttcatg ctgttgaatt attggacact
     9121 tgttgaaagc aacaatatcg ctcgtgaaag aaaacaagtt ttccataatt ttgaaaaatc
     9181 agcttatgct gacggcactt atttcgaaaa atacgtaact ggagaattcc agccaaaatc
     9241 agaaaaagtc aaagcattgt ttgaaggcat ttttatccca actgctgaag attggaatgc
     9301 tttgaaacaa gcagtaatga aagatggact gtatcatcaa aatcgcttgg cagtagcacc
     9361 aaatggctca atttcatata tcaacgatac gagtgcaagt attcatccta tcactcgcat
     9421 gatcgaagaa cgccaagaga agaaaatcgg taaaatttat tacccagcgc cttatctatc
     9481 caatgagacg attccgtatt acacttctgc ttatgatatg gacatgcgta aagtaatcga
     9541 tgtttatgca atggcacaac aacacgtcga tcaaggcatg agtttgactt tattcatgcg
     9601 ttctgaaatc ccagaaggca tttatgaatg gaagacaaca acgaagcaaa cgacacgtga
     9661 tttgaatatt ttaagacatt acgctttcca caaaggaatc aaatccattt attacgtacg
     9721 tacctttact gatgatgcag aagaaatcgg aagcaatcaa tgtgaaagct gcgtgatcta
     9781 atatcataga tagattcgct tattcatatt ttaattaaag gagcattctc atgtcagaaa
     9841 catattatga agcaataaac tggaatgaaa ttgaagatat cattgataaa tccacttggg
     9901 agaagctgac agagcaattt tggctagata cacgtatccc actttcaaat gacctagatg
     9961 attggcgtac attgtctcaa ttggaaaaag atacagtagg acatgtattt ggcggtttga
    10021 cactgctaga tactgtccaa tctgaaagtg gcatggatca gttgcgtaaa gatgtccgca
    10081 ctccccatga agaagccgtt ttgaacaata ttcaattcat ggaatccgtt catgccaaaa
    10141 gttattcttc tatttttagt acactgaata cgaaaaaaga aatcgaagaa atttttgact
    10201 ggacaaatac gaatccttat ctgcaaaaaa aagctgagcg tatcaatgaa atctacaaac
    10261 acggtacacc gctagaaaag aaaattgcca gcgtcttttt agaaacattc ttgttctatt
    10321 caggttttta tactcctctt tattatttag gaaacaacaa attggctaac gtagcagaga
    10381 tcatcaaact aatcatccga gatgaatctg tacatggaac ctacatcggt tacaaattcc
    10441 aattaggatt caacgagtta cctgaagcag aacaagaaac gttaaaagac tggatgtaca
    10501 atcttcttta tgagctttac gaaaacgaag aacgttatac agaagaatta tacgatccaa
    10561 tcggctggac agaagaagtc aagacattcc tacgctataa tgccaacaaa gcattgatga
    10621 acctagggat ggatccactc tttccagata ctgccaatga tgtcaaccca atcgtaatga
    10681 acgggatttc aacaggcaca agcaaccatg acttcttctc acaagtcgga aacggctatt
    10741 tgttaggtca tgtagaagcg atgaaagacg acgattattt gattggattg gaataaggat
    10801 ttattttaat tcaaaaattt ttcgaaaaat aaaaagagtt tagcgtacag atattttaaa
    10861 tgtacactga actctttttt atgcttattc acttgtttct tccagcactt ttggactgtc
    10921 taacttttcc gaaacatgtt ccattcttct caataaatag attttccgga tatttaatat
    10981 tatcgtctta atcactgcat agacaggtat gcaaatcagc atgcctaata ttcctgcgac
    11041 acttcctgct ccaatcaata ccaagatgat cgtcaaagga tggatattca ttgatttccc
    11101 aaataacaac ggcttgatca aatttccatc taattgttgg atcactaaaa tcgacaacgc
    11161 catatacaaa gcttgaaatg gagagataaa caaaccgaca atgattgctg gtaccgctcc
    11221 aataaaaggg ccgacatacg ggataatatt cgtgattccg caaatgaccg caagcaacaa
    11281 gctataaggc tgatcgaata ttctcattgc caaaaagctc attacaccaa taatcaaagc
    11341 atctaagatc gtactgctga tataggcaga taatgtttcg tttaattctt ttaccgtttg
    11401 gcgtaactct ttacgaatcg ctttcggaaa gaagtgagac aacgcatcaa taaatttgtg
    11461 cccatccttg aacataaata gcaaaataaa tggaacggta aataacaaaa taaaaaattg
    11521 catcataaat gaaatgattt ttgagacact tgcagataaa ctgactaata tgatattgaa
    11581 caaattacta agagataaat ttgctgatgc taattgttct tccaaattga aatttttaaa
    11641 ctcttcccgc tgcgccaatt catttaacca tttgcttgtt tcatctgcat acattggcaa
    11701 gctctgggtc aattgaattg tctgttccat taactgaggc actacattca tgacagttaa
    11761 aacaactaaa atgattacga ccaaaaacgt caataaaaaa ccgaacactc tcgggatccg
    11821 atgcttttcg agaaaaacaa cgattggatc gaacatataa tagataaaac cagcaacaag
    11881 caatggtaaa aaaactgccg aaagcatttt caatattgga ctaaaaatat tcggcatttg
    11941 taaaataaag tacacgccga tgatcgtgag tactagctcc actgtccaga aaaataattt
    12001 tgattgttta aaacgtgatt gcatacatct cctccaaggt cctttgatac ttctattcta
    12061 tcatttaaca aagagttacg cgctgagatt ttcatttgct taaaaaaaac aaaaaaaata
    12121 aacatatttt aatgagtaga cgagtactta aacaaaaaca cggtttggag tagtgacaaa
    12181 aattatactg cagtcccttt ttccaaataa acggtgttca aaagcttgca tcttcgtaat
    12241 aagcccagcc aaacgaaaaa tataaggaac tatttttatt tggctgggct tattacttga
    12301 tgtaggacat ctattttaca acctcagatc atttattgcg caatactgac taaatgcaac
    12361 tttgtgatat ttttgtgtac ggacaactcc tcgatcacat cggcataagt gagccctttt
    12421 ggaagatcaa tcgtatagat atttttgtac actcggtagt catcaacgaa ttttacgtca
    12481 aaattcacat cttcgattac gatatttttc tcttcaaaat aatcggtaag aaaatctttg
    12541 gtttcttttc tatggataaa ttggatttct aactttttag tcgtaggtac atggatcacg
    12601 cgtttcacta ctgtgagtgc aatagcaatc ccgataaaac tgatgatcgc aatggaatag
    12661 taacccatgc cgatggcgat ccctaatcca gcaattgccc ataaagaagc ggcagtcgta
    12721 agtcccgtca ccgattgttt cgtgacgatg atcgttcccg cgcctaagaa accgacacca
    12781 ctcaccactt gagcaatcag ccttgcttca tctgaacgaa tcgttccagc aagctgtgga
    12841 tactctaaag catctcttaa tgcaccacta gcgatctcaa cttggatcaa tgcaatgata
    12901 catgctccca tacaaactaa aatatgcgta cgcatacctg ctggacgatt tttatattgt
    12961 cgttcaaaac cgattgctcc gccaaatagc atagcgatcc ctaaacgtaa aataatctct
    13021 ggaattgtca ataccatatc attcatctaa acaccctcca gtccaatata tcatgtctga
    13081 aaggagcaga caaaaaaagt gcaattttta cctcttacga aaaaaggact gtgacaaaat
    13141 ttttgtcata gtccttttta tgaataaacg gtgttcaatc agctgaccct cggtattact
    13201 caacaaacgg caaaatatgg ggagctattt ttcctttgtc ttctctaata ctcaggtcaa
    13261 aacgctgatt tcacaacctc tttagcctac tattgaaaat tacaacttac ttgctgcttc
    13321 ttcatcgatg atcacaacaa cattgtcgtg attttgcaat acacttgctg gcaaatgatt
    13381 cgttacaggt ccttcaatca tccctttgat tgcatcagct ttgctttcgc caaatgccat
    13441 caagacgatt tctttacttt tcataattga accgattccc attgaaacag cttttgttgg
    13501 tacatcttct gctttatcga agaatcgttt gtttgcattg attgttgatt ctgttaagtc
    13561 aactactgaa gtcgttccgt caaatgaagc accaggttca ttgaaaccga tatgtccatt
    13621 ttgcccgatt cctaagattt ggatatcgat tggatgtgca tcgatgattt gatcatactt
    13681 tttgcatgct gcttccagat cttctgcttt tccatctggt acaaacgttt ctttgaatgg
    13741 ttttttatca aataattgga catccatgaa atgacggtag ctttgttcat ctgaaccgcc
    13801 tagtccaacg tactcatcta agtttacaga agtcatttca ctgaaatcca aatcgcttga
    13861 agtcatttct ttatataaag taactggtgt gcttcctgtt gccaatccta atacttttgc
    13921 acctttgttc ataccttctt tgatgatttc aaatgctttt tttccgcctt cttcagcgtt
    13981 ttttacacga atgatttcca tagtgattgc tcctttatat tttggtatag cccaattata
    14041 gcattctttt tttcttttta caataattag tatagaccaa attgcatttt tttcattttc
    14101 ttgacaataa ataggaatcc gtgttaggtt tcataagatg gttcaatcaa acaatccatg
    14161 ttgcatgaaa accaagcaag tgattcactg tggacgcttt atgtctgcag tttttttatg
    14221 aaaaaggagt tattatgagt atactgacat taaacaacat tacccagcaa tttggcgaca
    14281 aaatcctcta tgaaggtgtc gatctgcaat taaatgctgg cgaacatctt gggctgattg
    14341 gacaaaacgg agccggtaaa agtacattga ttaaaatcat cactggtgag atcttaccag
    14401 atgacggaca gatccattgg caaaaaaata ttcacaaagg ttacctcgat caatatgtgg
    14461 aagtcgatga aacattaaca atcgaagaat ttctaaaaac tgcgtacgca aaagaatacg
    14521 aaaaggaaga aaaaattgct tttctttacc aaacatatag cgagaccttc gatgattcct
    14581 tgctggaaaa agctgggaaa ctccaaacgg aattagacca aggagtgttt tatcaaatcg
    14641 acaccttagt tgctgagatg agcagcgggt tggggatcga tgtcttaggt ttagattcac
    14701 tgttgaaaaa tctaagtggt ggacaacgtt caaaggtgat tttagcaaaa ctacttttag
    14761 aaaacccaga tgttctgatc ttagatgaac caaccaatca cttagatgat cagcatatcc
    14821 aatggctgac gcagtttctt caggattttg acggtgcgta tatcgtgatt tcccatgacc
    14881 aagaatttct tgatcagatc accacccata tcgcagacat cgagtttgga aagattacta
    14941 agtatactgg ccatctaaaa caagcattga aacaaaagga acaaaatcgc gaaagctatt
    15001 tgcgccaata tcatgctcaa caaaaacaaa tcgaaaagac cgaagcttat attcgaaaat
    15061 acaaagccgg ttcccgatca acgatggcaa aaagccgaca aaaacaatta gataaaatcg
    15121 aacggctgac gcctccagct tcagcaagta aagctgtctt tgattttcca ttctcgccta
    15181 ttgtcacgac attagcagtg gaaacaactg atttagtcat cggatatgat cgaccgctac
    15241 ttgcacccat caatttgacc atccgctttg gtgaaaatat tgccattcga ggcttcaacg
    15301 ggattggaaa atctacttta ttaaaaacac tgatcggtga gattgaaaaa ataagtggcg
    15361 atttccactt cccagataac acaaaaatca attacttttc acaagatctg tactgggaaa
    15421 atccattaga aacaccacta caatatttaa gcagtcaatt tccaaaagcg actatcaaag
    15481 aattacgtcg ccaactagct aaagcagggt tagttaatca attagcaagt gaacctctca
    15541 ctacattaag tggtggggaa caaactaagg tgaaacttgc acagttgaca atgaaccaag
    15601 gtaatttact gattctcgat gaaccgacga accatatcga tcaagaaacg aaagaaagct
    15661 tacaagacgg gatacagaaa tttccaggaa cagtaatcat cgtctctcat gaacaagaat
    15721 tttatcaaga tcttgtgcaa cgagtcattg agattgaacc aaaataaacg tttgattgaa
    15781 tagttgaaaa aaaggagctg tgacaaaagt tttgtcacaa ctccttcttc tgaataaatg
    15841 gtggccaatt agctgacctt cggtattagc tcaaaaaacg gcaaaatatg aggagctatt
    15901 tttatttggc tattcttatt acttgatgca gaacagcttt tcacaacctc ttttcgtcta
    15961 ttttttattt tcgagatttc tctttccttt aaaaacaact gtttgaaaga agaacgcaca
    16021 tccttgaaaa acaagcccgc taaccgttac acctaaccag ccatactgct gccacattgt
    16081 cgtccctagc aatgaaccag ccgcacctcc aataaagtac ataaacataa atacagtatt
    16141 atttcggttg cttgctgctt cacctagccc ctgtactcgt gtttgattgg cgacttgacc
    16201 aaattgattc ccgacgtcta ataaaataat agctaaaagc aaaatggcca aatggcttcc
    16261 gccaaccaat aaagcaataa agcttattgt ttgcataaca agtcccatat acacgatttt
    16321 tctttcagaa taagaatctg ccagtcgtcc cacgataggt gcagcaactg ctccagacaa
    16381 accaaaaatc gctaatatcc ctgcttcgaa tgttccccaa ttgtaggcgg gactgctgat
    16441 ataaaaaatc aacgtagacc aaaagatcga aaatgttcca aacatgaaaa atccactgac
    16501 tgccgcttct cttaatagac gttgagaagc aactaaatgc ggcaaacttt taagtgaatc
    16561 aaggtatgaa acagctgtac tactttgctg gacagaacgc ggcattttcc agtaaagcag
    16621 aagtgtaaga attccgactg cacataaagc aaataaatag atcatccgcc aagaaaaaag
    16681 actggcaaca ataccagata ccgtccgaga aagcaaaatc cccacgagta aaccgctaag
    16741 caatgtaccc atgaccttcc ctcttgcttt tggtcccgct agtaccgcac cgtaaggaat
    16801 gatgatttgc ggcacgatcg aaagtaatcc tattaaaaaa gaagcgcatg caaataacgt
    16861 aaacgaaggc gcaaaaaaag cggccaacag agagacggca gataacgctg ccattcgaat
    16921 gatcaatttt ggacgatcca ccacgtcccc taaaggaact aaaaacagta accctaacgc
    16981 atagcctaat tgggtcaaca tcgtcaatat cccgactgca cttgtgctaa cagataaact
    17041 agctgctact tttgttccaa tcggctggat gtaatacata ttggcaacaa caacgccaca
    17101 agtgattgat aatagtaaaa taagcgaagt tgttaaaggt tttttttcat tactcttcat
    17161 tgatttcccc catttcctat cttagtttac aacttatgga aaagattgga aatagaaaaa
    17221 tacaactatt cgccacactt attatttgtg cttttgtttt ccttgttttt tcttgcgatc
    17281 tttataaatg aagacaacta accctactag cacagtaaca cttgcaacca acgtgactgc
    17341 agttttgtta tatccgtgaa acagagcttc tccaccacac gcatacaaca cagacgaagg
    17401 gatgactcca atcacgatcg acaatatgat ttgctgtaga ggcaatttca tcgcatctgc
    17461 agcaaaattg accatagaag atggaataac cggtatcata tagcctaaca ttacacctat
    17521 ttttggatgt ttcatttgtc ggattgcttg cacccaatga ttcgtttcgg ttttcttatc
    17581 taaaaatttt aagcgatgca taaggaaaat tgcagtgatg ttaccaagag cattacccaa
    17641 aatgttgatg atcgcacctg tcagtggtcc aaagctcaaa ccagcaatga ctccaactac
    17701 tgaagtagga atacctggaa cagcacacat caccgctgtc aatacgatca agagtatcga
    17761 tgcaaaagtc ccatgcgaac gaatatgtgc gagtagctct gccttactcg ctctcggatt
    17821 taaaaataat tggatgtctg tctgatattc taaatacaac cgatagccca ctaccagtat
    17881 cagaatgatt cccaacgaga tcaatatcaa tcggattatt ttctttttca tatttcctcc
    17941 tgaaaaaaat ctatattcat taaataatag tttaacataa aaacaaaaaa gcaccgaaat
    18001 ccaacatagg atctcagtgc ttttaaatag taaaaattat ttaacagtta cagatgcgcc
    18061 aacttcttca agtttagctt tcaattcttc agcttcttct ttagatacgc cttctttaac
    18121 tggtgcagga gcgccatcaa ctacagcttt agcttctttc aagcctaaac cagttgcttc
    18181 acgaactgct ttgataactt taactttttg gtctccagct gcagttaatt ctacagtgaa
    18241 ttctgtttgt tcttcagcag cagcagcgcc accagcagca gctacagcta caggagcagc
    18301 agcagatacg ccaaattctt cttcaatagc tttaactagg tcgttaagtt ctaggatagt
    18361 tgcttctttt aactcagcaa cgatgttttc aatgttcaat gccattataa tttcctccgt
    18421 ttaattgttc ttttttattt aatgctttat gcggcaatta agctgcatct ccatcttctt
    18481 tgtctgccac tgctttgaca gcgtaagcca cgttgcggac tggcgcttgt agtacagata
    18541 gcaacataga tagaagtcct tcgcgatttg gcaattttgc caatgctgtg atttcttcca
    18601 atgaagagac tttaccttcg atgataccgc ctttgatttc taatgctttt gcttctttag
    18661 caaattcgtc catgattttc gctggtgcga ctacatcttc gttactgaat gcaacagctg
    18721 taggtccagt gaatacttcg tccatgcctt caaggccagc ttttttagct gcacgtgaaa
    18781 gaatagaatt tttgataact ttcatttcta cgttagcatc acgaagttgt ttacgtaaac
    18841 gtgttacttc atcaactgtt aatccgcggt agtcaacgac tactactgat gcagctgctg
    18901 taaatttttc gcttacttca tcaacgattg ctgctttttt tgcgataatt gcttcactca
    18961 tttttgagtt tcacctcctg aattttgatg gaagagtttt ttgcactgaa aaggcaagtc
    19021 acttttatag ttactttgct caaccagtat tattcatcac ctattcactt cgttcatagt
    19081 gattctactc gctttgctcg aaaggaaact cctttcgctt tgctcaacca gtattgttca
    19141 tcatctcttc acttcgttca tagtgattca caataaaaaa ctctatgcca ccgcagacat
    19201 agagggacta ttgaatatat tcaataaacg tcctcggtag gaaattaagg cttgatgcca
    19261 cctactgtct tcggtacaag ttaattatta acctcaacta gcttagcatg ctaaactagt
    19321 tgagtcaaga attatttttc agttggatca atcaacattc taatatttaa ttagaaagaa
    19381 gcttggtcaa catggatacc aggtccaaat gttgttgtga tcgttacatt tttcatgtat
    19441 tgacctttag ctgctgatgg tttagctttt agcaatacat catggattgc tgcaaagttt
    19501 tcaactaatt tttcatcgtc aaatgaaact tttccgattg gtacatggat atttccagct
    19561 ttgtccacac ggtaagttac tttaccagct ttcacttcgt ttactgcttt tgttacgtcc
    19621 attgtaactg taccagtttt agggtttggc attaagcctt ttggtcctaa tactcgtcct
    19681 agacgaccta cagtagccat catatcaggt gttgcaacaa ctacatcaaa gtcaaaccat
    19741 ccgttttgga ttttttgaac catgtcgtcg tcacctacga aatctgcacc agctgcttca
    19801 gcttcttttg ctttgtctcc tttagcaaaa actaaaacag tttgtgtttt accagttccg
    19861 tttggtaata caacagcacc acggatttgt tggtctgctt ttttagggtc tacgttcaaa
    19921 cggtatgcaa cttcaactgt tgcatcaaat tttgcaatgt ttgtttcttt tgctaaagct
    19981 actgcttcgg caacaggata aactttactt gaatcaactt tttttaatgc atcttgcatt
    20041 tttttgctct ttttagccat tttggttcct ccttgattgt ggttataacg gttgtacctc
    20101 ccacgtgttc tatatctttc aatatagagc caactgagaa gctgctgtct tagggtgtat
    20161 agattattct acagtgatcc ccatgcttcg tgcagttcct tcaaccatgc gcattgctgc
    20221 ttcaacgtta gctgcgttta ggtcttccat ttttaattca gcgatttctc ttacttgatc
    20281 gctagaaact ttcgcaactt tatttttgtt tggttcgcct gaaccttttt cgattttagc
    20341 tgcttttttc aatagaacag cagctggcgg tgtttttgta acgaatgtga atgaacggtc
    20401 ttcgtataca gaaataacaa ccggaatgat caaacctgct tgatcagctg tacgagcgtt
    20461 gaattctttt gtgaatccca tgatattgat acccgcttga cctagtgcag gacctactgg
    20521 tggagctgga gttgctttac ctgcaggaat ttgcaattta acgatttttt ctactttctt
    20581 tgccacgaga catacctcct taagtccgtg atgtggttaa ttggagatgc atatttctcc
    20641 tcccacgttt tatctgatct gaaaaaatcc agacaaataa tgtgcaaaac aatgcacact
    20701 ggtacagtat agcaaattta tttgtttttt caagattttt ttctgctatt tttacatttt
    20761 gcttgaataa acgctaaatt tattctaaaa aaacaacaat ttttcgttct catttcttct
    20821 tttatgttac acttgcgcta gcatggttca acagatgaaa gcaaacaata acacggtttt
    20881 ttagaccctt gtacaaagat tcctacataa caaaatgagg tggagaaaat gaaagattta
    20941 aggtatagaa gtgtctttga tattattgga cctgtgatga ttggtccaag tagctctcac
    21001 actgccggag ctgctagaat cgggaaaatc atacgtagta tatttggcga acagcctgat
    21061 tctgtagata tttatctata tgaatcattt gcaaaaacct atcgggggca cggaacagat
    21121 attgcattag ttggcggcct gttagggatg gaacccgatg atgaacgttt agctgattct
    21181 ttgaaattgg catatgaagc cggtatggaa gtcttgtttg ttcctaagag tgaaaaagcc
    21241 gaacatccaa actctgtaaa gatgcttcta aaaaaaggaa cacgaaagct ttcagtcact
    21301 ggcatttcga tcggcggtgg caatatccag atttctgaat tgaatggatt caaaatttct
    21361 ttaagcatgg gtactcctac cttgatcgtt gtccaccaag atgttccagg tatgattgct
    21421 aaagtgacta gtatactttc tgagaccaat acgaatatcg gcactatgac agtgacacgg
    21481 gaatcaaaag gtgaaaatgc cattatgata attgaagtag atgatcctca agtagaagaa
    21541 acggtaaaaa gactcaaaca gctgccaaat atcgacagcg taaattattt cgattaagga
    21601 gctaaaaaaa tgtttttatc gattgaagaa ttagtagatc aagcaaaagc ttatccaagt
    21661 gtagcagaat tgatgattgc agttgaaatg gaaatgagtg gccgttcccg tagtcacatc
    21721 atcgagatca tggaaaaaaa cctctcagta atggaacaat caatcaacga gggaaaagaa
    21781 ggtgttacct ccgtcacagg aattaccggt ggtgatgcaa aaaaattaga tgattattta
    21841 aatgaggcag actttttaag tggagaaaca atcttaaaag ctgtacgcaa tgctgtagct
    21901 gttaatgaag tcaatgcaaa aatggggttg atttgtgcta caccgactgc cggaagcgcg
    21961 ggtgttgttc ctggtgtgct gatggctgtc cgagatcggc ttgatttgac acatgaacag
    22021 caattggaat tcttatttac cgctggcgct ttcgggttag tcatcgcaaa caacgcttct
    22081 atcagtggtg cagaaggggg atgtcaagca gaagttggtt ctgcaagtgc aatggcagca
    22141 gcagctttgg tttgtgcaaa aaatgggact cctcatcaag cagcacaagc agtagccatt
    22201 acattgaaaa atatgatggg actgatttgc gatcccgttg caggcttagt tgaagtcccg
    22261 tgtgtcaaac gtaatgcttt aggctcttct caagctttta tctctgctga tatggcatta
    22321 gcgggtatcg aaagtgtcat tccgccggat gaagtcgttg ctgcaatgta ccaagtcgga
    22381 cgtcaaatgc cgtcgatttt caaagaaaca gcagaaggtg gcttagctgt tacgccaact
    22441 gctaaacgac ttgcggaaga gattttaaat aagagggcgt gataaagctc cttaatttcg
    22501 agtattaaga gattagttat cttcataaca aaaaacgaag agaggactaa ttgcccttct
    22561 cttcgttttt tattaatcca tcaatttcgt atattttact tcatcgatcg tttttgttcc
    22621 tgcaagttgc atcgtgatcg acaattcttt gttcaggtgt tcaaaaacag atgttactcc
    22681 ttctgcccca cctagattca acccataaat cactggtcgg ccaatcgcaa ctaagtcagc
    22741 accacttgcc agagctttga agacatgctc tcctcgacgg acaccagagt cgaaaatcac
    22801 tggtacacgt ttgttgacta cttcggcaat ttttggcaat acttcaaagg acgcaggacc
    22861 tccatctagt tgacgtccac catgattgga tacccagata ccatctgccc ctgcagagat
    22921 agcgattact gcatcttctg gcgattgtat tccttttacg ataactggaa gatgggtgat
    22981 ttctttgatt gttttgatgt catctggtgt caagccttgt tttgcagctg cataaatttc
    23041 tgcgattcct tttccttcgc cattaccact tgcactttgt tcgctatacg cagctaagtt
    23101 aggcattggt aatggaaatt ggaattgatt gatgacgtct tcttcacggt aaccaccgag
    23161 cgttgaatct gccgttaaaa tgatagcttt agcccctgct tctactgctt tgttcaaaat
    23221 gaactcattg aatctgtcgt ctttactcat atataattgg aaaaactgcg gtgcgcctgg
    23281 cgctgcatcg gctgcgtctt tgattgtcgt atttgcataa gtgctgatgg aaaagattga
    23341 tccagcagca gctactcctt tagctgtatc agcttcccct ttttcatgtg ccaatccttg
    23401 ggccgctgat ggcgcttgga taatcggtgt atctaattca attccgaaaa cagatgtatg
    23461 caaatcggct gagtcgattc cgcgtaatat ccgtggcatg attttctttg tattaaaaga
    23521 agtcgtattt tctttcatcg tccactcatc ttcagaacca ccgcgaatat agccaaaagc
    23581 tcctgcttcc atgcgctctt ttacacgacc ttctagttag gcaatgttta caatatcgat
    23641 tggatgttct tcattacttg tatgataagt catagtagat ccttctttca tcttcttgat
    23701 tacatataaa tcatactaaa cttacaaaaa gtaagcaaac atttactctc agatactttt
    23761 atcgctattc tttccaaata aatcgactta caagctgatc tacttcttgg ttttcatgaa
    23821 gcatactatg agaggcattt tcacctgtca ctactttttc ctctatttca tttccgcgtt
    23881 gcttcaacaa cgcatagacc gctaatgcac tgtttaacgg tactgttcca tcactcaagt
    23941 ctgtttcatc tagttgacca gcaatcaaga aaaatcgagt ggttatagga atattaccga
    24001 tcatttgctg gtaatcttga tagcggttag aaacgacagc aggccccttt gctaactcat
    24061 ctgtcagaga ctgcgcacta tcatcgacaa aatcattaaa aggcgcgcca attgctgtta
    24121 atgtctgaac ttgtggcaat gacgtatcct gtccatacgt tcctaaatag cgtagtgcac
    24181 taactcctcc catggaatgc cccactatgt tgactttatc tatgccgtag ttttctttca
    24241 gatacaaaag acaagttttg atccattctg cttgattcca ctcgttattt ttattatctt
    24301 caaacaatac ttggaccatc ggattgtctg cttttccaga caaatctcct gttgcttgta
    24361 tttgtccatc tgttcctact gttaaaacca attcttttac tgcttgctga tttttttcca
    24421 aacgccgcaa catcccttta aaggaatttt ctgtcccgct gtagccgtgg aaaaaaagtg
    24481 ttggaatagt ggttttttct atcactttac tggattctgc cgttttttct ttatcagaaa
    24541 cttcagtagt atttatcttc gtcgtctggc aaccacttaa cgcaaacaaa ctgataagga
    24601 ctaaagctgc tgcagttgca cgtttgcata aatttttgat tggttctgtc atacacctct
    24661 tcctttccat atagaaaaaa gaaagacttg aaaggccaat catatgatcg acatttcaag
    24721 tctctttatt aatcgatttt atctacttga tcgaaatcaa gttctgtact ggtttcacgt
    24781 ccaaacatat cgatgttgac tttcaatttt tctttttctc cgtcaacttc tgttacttgt
    24841 ccttctaatc cagcaaatgc gccttcgatg attttcacga cttcgccaac ttctacttct
    24901 agatcatttt gtcgcaggct cataccgata gaacgcaaga tatgattgac ttcttctgga
    24961 agtaatggtg ctggtttgct tccggctccg tgtgaaccaa cgaatcctgt cacaccaggt
    25021 gtgttgcgga caacatacca tgaagcgtct gtcatgatca tttcaacaag tacgtatcct
    25081 gggaatgttt tatgaacgat ttctttttct ttcccatttt tgacttcagt ttctttttct
    25141 tcaggaacaa cgacacggaa aataaagtcg gccattttca tactttgcgc acgagattcg
    25201 atatttgctt ttactttgtt ttcatatcct gaatatgtat gcaagacata ccagtttttt
    25261 tcaaaagatt ccattgaagg ctcctcattt cattgtaaaa taaaaaaacc tcagtacacc
    25321 cgaagttttt ctttcgcttc attatagcac taaaagtacg ttgtggctag cgagttattt
    25381 taaaatccag ccaaataagc tttggatggc tgtatccatt acgtaaaaca tcaatccgaa
    25441 aataatagaa gtttcgatga ctactaacgt atctttacgg agttgttttc tgcttggcca
    25501 agttacttgt ttgatttcat cttttacgct ttttagaaat ttcatgagac aacctccctt
    25561 gctactgttt atttcgtttc tttatgtaat gtatattgat tacaatattt acaaaactta
    25621 ttgatttcca aacgttcgcc gctttttcct tcactgactg attttgtgta gttgcgagac
    25681 ccacaaacgg aacaagcgag tgctgctttt ttctttgcca tttacttctg cccccttgct
    25741 gagaatcaga cttatacttc ataacagtat cacgtaactt tttacttgtc aatttgtgaa
    25801 ataaactatc tattgttttg gataaaatta tgatatgatt aatttgaaat taagctaaaa
    25861 tgagacaatt ggaggtatag cgatgctatt aaaaacactt gtatataaga aaaaagatct
    25921 gacaaccgtt acagagacta gtacattaga agaagcatta aaaattttag aaacttccgg
    25981 ataccgttgt gtgccgatct tagattcttc aggtaatatt ttccgtggaa acatttacaa
    26041 aatgcatatc tatcgtcata aagcaaatgg cggtgatatg acgcttcctg tcactcatct
    26101 tttgaaaaac gctacaaagt tcatttacct ggatacttct ttcttcaaag tattctttac
    26161 gatcaaagag cttccttata ttgctgtcct tgacagtgaa aatcattttt atggtatttt
    26221 gacacatagt acgttgctta atatgctctc tcaatcttgg agcgtagaac aaggaagcta
    26281 tgtactgaca attgcttcca caggaaagca aggagatttg gctgctattg caaaaatcat
    26341 ttcaaaacac agcagcattg ccagctgtat tactcttgat atcgaaaaag acgaatacat
    26401 tcgacgtaca ttgatcacgc taccggctaa cactgaagcg aaagtatgtg aaacgatcgt
    26461 caaccactta gagaaaaaga attataaagt ggtagaaatt gaaaatttgc aaaaccaaga
    26521 agcataatca aaaagcgaaa aaagaaaaca gctaatctgc tttctttttt cgctttttat
    26581 aattagacac gcttttcaaa atcaagcgcg cgatctaaat aatacatcaa tggtgctgaa
    26641 acagccatga cgataaactc acttaatgca agcgtgccat aggttgtcca aaatgcttgg
    26701 ccacctgttg gaactaacat ataagcaatc agtcccatgc tgatggtaaa gaagatcgtg
    26761 ttcaatacta aacgcatctt tacatttttc actttgtttt gcaataaagc tgtcaggcct
    26821 aaagaaagca atgtttggcc gccaccaaaa accacatcca tgataccaaa accaaagaag
    26881 gcattataga cgatgacccc gcctaaaact ccccaaagca atttacggtt aaagacaact
    26941 aaatgattca aactttctga aatacgaaac tggatcggcc ctgaggatac aggcgcaact
    27001 aaaatagtta acgctagata aagagccatg atgatgccat ttacaacgat tacggataac
    27061 cgttgtctgc ttttcgtttt ttgcattttc tctcctcttt tctcctagtt tttttaacgc
    27121 gggatggttg atgaaccgcg gttatgtgaa acaaacaata attataacag acttttcatt
    27181 tatttcaatc cgtcttatct gcttttaaaa aagcaaaagc ttaaacaatg taaacgtttg
    27241 ctaaaaatcg tgggatatgg tataaagtaa gtgtaagggg tcataagaat tcccagtaaa
    27301 gcagacgaag taggatctgc aagaacagaa ttgttgtgaa acttaaataa ataaaaaagg
    27361 atgtgggaat ttgagcaacc tcttactaga gaacggtcta gcaagcagct gaagatctaa
    27421 gtggacatgc cgaaccttgg aacttctcct tacgtaagta atggatcatg cgcttgctag
    27481 gcttttttgt gtttttcggt cttcggacct ttttcatttt cctagacttc tgctataata
    27541 accaagaatt aattttgtaa cagatttttt tgaaaaaaca agtttctttc attcaaaaaa
    27601 tagaaaaagc ttgctaatta ttggttaacc agcaaacagg agtattaaaa tatcttgttt
    27661 tattgaatag caggaggaaa tcatgaaatt ccattatcat atattgatca atccatcggc
    27721 aggcagcgga aatggctata aagtagcaga aagaatttta ccggtattaa aaaataagca
    27781 tattgactat acacttcatt atagtgaata caaaggacac gatgctgaga ttgctgaaac
    27841 tttggcaaaa gaaacattga ttccttggat agaagaagaa cagaaaacaa tcgatacttt
    27901 cccccttttg atcatcgtag gaggtgacgg caccttacat caggtacttg atactttcta
    27961 tcagatggaa gtagaattcc cagttgctta tattccagca ggttctggag atgattttgc
    28021 tcgaggggca ggccttccaa aaaatcccaa aaaaggactt cagctgattt tagcagctca
    28081 atcaccagaa aaagtccaca taatggctta tgaagaaaaa ataagtgaaa aaaaaggaat
    28141 agctgttaat aatttcggta taggattaga tgcagctatc gtccatgcga caaatcattc
    28201 ttccacaaaa aagcgattga acaaatacaa cttaggatca ttttcgtatt tattctctat
    28261 tctccgtgct ttattcactc aaaaaggatt tccaattctg gtagatgcag gaggaaaaca
    28321 attaagtttt tcaaacgcat ttttatgtac agcaacgaag catccttttt ttggcggagg
    28381 aatcgcgatt gttccaactg ctgatgcgtc taaaccagtc attgattttg tcgtggtcga
    28441 acgaatcaat ttctttaaaa tcttgtggct cattctttta cttatacaga aaaaacaatt
    28501 gaaatcgaaa tatttccatc actacacaac aaacagatta cgtatcgttt caacaacacc
    28561 gcaacatggt caagaagatg gagaagaaat ggaaaaacga ccttttgata tcacaataac
    28621 cacaactaaa cagttgtttt ggtgttaata aaagaggttt tgtcagaagc tacatctgga
    28681 acaccgttta ttcggaaaaa aggactgtga cacgactttt gtcacagtcc tttttttact
    28741 atgcagttaa ttctttgccc aattgggttg ccatcgatag tttctttctt tacccaaaat
    28801 aaattggatc attgaaaaca ccgcagcaaa agcaactaag acacaaccat tgaaccaaaa
    28861 agattctgca aatgtttgat tgtaactgag tgtttcactg atcaagttat tattttgaac
    28921 aaaccaatat gttggtgtga atgccgctat tttgctaacc atttctggca agtattctgc
    28981 tggtacaaac accccaccga taaagcaact gcctgtgata aaaatatctt tgatcccacc
    29041 gctggtttct gagtttttta ccaatgaagt gatacaagcc gaaaagctaa tgatcggcaa
    29101 taaaaacaag aaagtattta aaatatgata ccataccgtt atatcccacg aggtgtggat
    29161 cactaacaga accattagaa gtaagaagcc actgaacaac agcagcgagt aactaatcaa
    29221 actaaaggtt actttgcgat tcaagtttcg tttggtaatg ggcgcactct ggtttcgttt
    29281 actgatctct ttgcgattaa atgctagatt gatgacgctg aaaccgctaa aaatggatag
    29341 aaacaaccca taagacaaga aattaaaggt tgtgcccgtc aacagctttt tcatccgctg
    29401 actatatccc gcacttagct caacttctcc agtaaccgag agtgccattt gtgtctgttc
    29461 catagctgca tcgattgttt tatcttcttc ttgaaacaga atcagtgttt gcaaaaaggt
    29521 gttcacttgc tgtgtaacaa ctgttttaga aaaggcatct gggcgactct gtatcttgat
    29581 cgtcggcatc tttcctactt cgacttgttc ggtaaagtct gcaggcagat gtaagataat
    29641 gtttacacga ttaaaataaa gagcatcatc gatttctttt tgtgaggttc catccaatgt
    29701 aacgactgtt tccttgtcat tcaaatattt acgaaggcca tcgactacag gcgactcttc
    29761 ttcactaatc aaggcgattt tcgcatcttg cagagaggta ttttcaacat caatattttg
    29821 cgcaaaaaag aatgtggcaa caacagtgat aattacacct atcagtaaat tcgatttatt
    29881 tgctttaagc aatgtccaaa tcgttttata aagcgtcata acgcacccct ctttccatcc
    29941 aagcatttcc taacgcaaaa aggaaaataa atccgatcaa ccatagaaga ttggtataaa
    30001 aactagccaa atcagtgtag aaataaagtt tatacaaact ttcgctgatc aagttgacga
    30061 gatttaattg accaacaatc ggaatatgaa gatcaatcca atacttcact tgttccgacc
    30121 ccatcatacc agcaaaaaag ctcatcgcaa tcgtcactga aatcaagatg ctatcctttt
    30181 gcaccagatt catttttggt atcaaattac caaccaatgt acctaacaaa atcgcacaca
    30241 acgaccccag agcggtaaca atcatcaacc attgccaacg ttcaccaaag tctacttgat
    30301 agataaaacg aaagacagct aacataatat aaatctctgt caaaacaatc gtaaagcttg
    30361 ctgccaaatt agcgattgaa actaacgatt gctttctagg aatcaaggac aagcgaatac
    30421 catttgacga ttgatttgct tgctgatcct gtacattgcg gaccccccac atcgtcccgt
    30481 agagaatcgc cattccaatc agagtgaaga aataaaaact tttgaaactc atattacgac
    30541 ctgcatgaac ttcttgaaaa gatacttctt gttcaaaaac agtcatgatg ggcgcgagat
    30601 caacgttctc cacttctgcc actttactcg aaattatacc aatacgctgt aaaaattgat
    30661 ccatgacgtt tttcatcaac gtctgactca ttcctgcttg acccacatgg agcgaaacag
    30721 atgaaccatc aatgagataa aaaccagcca aatcatctgc atttagcttt tcattcgctt
    30781 ctacttccga tagttcctgt acttcaaata atggtttatc attgacttcg acttgttcta
    30841 agactgtggt aaaattttcc gaaagcgctg tttgatccgc tttgattaca attccagtat
    30901 taatcgtttc gagattgcca ccttgatcaa tctcgccaaa ggccgccata aacatcaatc
    30961 ctaaaagaat tggaaaggcc aatgtccaaa acaacatgga tttatttcgc agcagtattt
    31021 ttgtgcgata tttataaaaa tgccaaaaca ttatttcccc tcctctagtc tctcaaatcc
    31081 ttgccagtaa tctctaaaaa gacatcattc aaggacgctt ctttggttgc tatttttccg
    31141 taactgatct tttcttcttg taagattgta agaatcggca acaatactgt atttagttca
    31201 gtagttgtta cttggagctg acctttttta taagcgattc catcagaagc tattgttcgc
    31261 tgacaacacg ttttctggga gtaatgggat gtcgatcgtc aaacgatccg ccgtccgtac
    31321 catatctttt agagattctt tggttccttt agcaatcacc tgtccttgat ccatgatcac
    31381 gatctggtcg caaagaattt ctacttcttc catgtaatgt gttgtataga caatggttgc
    31441 tccttgtcga ttcagctcct tgatactttc taaaatttta ttccgacttt gggggtctac
    31501 agctactgtc ggctcatcta aaaaaattaa ccgcggcttg tgggcaatcc cacatgcgat
    31561 gttcaaccga cgtttcaagc caccactaag ttctcctgga cgaaacgaaa catatttttc
    31621 taatcccact aattgaatca cctcttcaat caaggctttc cttttctctt tttcggaaac
    31681 atataaaccg caaaaataat cgatattttc ttgcacactt agttcgtcaa agaccgcaac
    31741 gtcttgagga acgaccccaa tcattgcctt gacagcataa ttttcaggag tcattgttgt
    31801 cccgaacaat ttaatggttc ctttgtcata atttaacaaa gaaagcatac agttgatggc
    31861 agtcgatttc ccactgccgt ttggtcctaa cagacctaag acttctcctt cttgtacttg
    31921 cagatcaaaa tgattcaatg ctacaaaatc tccatagcgt ttgactaatt tatcaatttc
    31981 gataatcatc atgaaaaact ccttccaaat ttcttgttga tctgactata ataaagtgca
    32041 agaaaagaaa ctagtgacga ttggctaaac cacacatgac atttgtcaca atgaaggagt
    32101 ctgctatgca taaatctcgg ctactcaacg aaactagctt tctactggtt atctcattgc
    32161 ttttgctggt aagtcaagga ttcgttgcta tgcaagttat ctatttattg atcgccgttt
    32221 tattcactgg tgtcagttat ttactgccta caaagccaaa ttggattttc caagttattc
    32281 tatttctttt atgtgccttt ttacctgatc ttgtcctgtt tctgccagcg acgatgcgag
    32341 tgatcgcccc tgaagaaagc caccttgatt acttgttttt gttaggtttt ttgttgattt
    32401 cgacggttgg taatcatccc cccatcaagg tcgcctttgt cgtaattttg ctgttttttt
    32461 ccttttactt acgaaaaaaa gatcaacgaa cgaccgatct tgagaaaaag tacattgtct
    32521 ccaaagatac tcattgggaa aaacaacaag agttatcgga acaaaataaa gcattggccc
    32581 tgtcacaaga aacacaatta gccttgcaaa ttgtggaaga acggaaccga attgcccgag
    32641 acatccatga caatgtcggt catctgctgt caagtgcgat tttacaaatt ggtgcgcttg
    32701 aaatgatcaa tcaagaacca aaactaaaag aaccattaga aaatttaagt gccaccgttc
    32761 acactgggat ggatcggatt cgtcaaagtg tccatgacct ccatgagaca tcgatctctt
    32821 ttcaacactc cttagagttt ctgatcgctg attttccttc ttctgttacg attgaaggaa
    32881 acttgtttga aggattgaat gaaacgcagc aaaactgctt cttaatggtc atcaaagaag
    32941 ccttaaccaa cagtatcaaa catagtcatg ccaaaaccgt tgttttacat tttcgaacgt
    33001 tgccggcctt ttatcgcttg caaatcacga atgatggcac cagcccacaa aaaaattctg
    33061 ctcccggtat tggtttgaaa acgatgcgtc aacgtgttca agaacaaaag ggacaagtcc
    33121 atattttcca agaaaaccag caatttcagt taacgatcat tttaccaagg gagggacacc
    33181 aatgataaca gtagtcatta ttgagatgat ccatttgtga cgaaatcgtt acaaaccatt
    33241 ttagaaagca cgaaagagat ccgtgtgtta ggcattggtc attgtgccaa ggatgcgcta
    33301 gtcttatacg aaacccatcg tcccgatgtt ttgctgacgg acatccgcat gcctaaacaa
    33361 acgggcatcg atgctgccaa agaaattttg gctaaatttc ccaaagccat ccttttgctt
    33421 ttgacgacat tcaaagatga agaatacatc cgcgaagcat tttccatcgg tgtcaaaggg
    33481 tatttaatca aacagaactt gcaagcgatt attccttctg tcaaatccgc ttataatgga
    33541 caggccgtat ttggtaatga aatcatcgag actttcactc aaatgatgaa gaataaaccg
    33601 acgattgata gtcatgctta tgcttctttt agtgaacgtg agcttgcaat catcaaagaa
    33661 gtagctgccg ggaaaaacaa taaagaaatt gcagatgcct tgtacttgag tgacggcact
    33721 gttcgcaatt acatcagcca attactagaa aagctagatt tacgggatcg cacgcaattg
    33781 gctatctatt tttatcagca tttgcaataa gtcttatcta acacaaaaaa acctgaatga
    33841 tgctccttcg catcattcag gtttttttca catcttctat tcaattgtcc cgcaaaccat
    33901 ttcctcacga ttgaaagaat caaggattcg cagggctgcc aggattcgtt ggattgttcg
    33961 gcgtgttagg cggaaaaata ggcgccttgt ctaacgtttt ttctagtttt gattcttcac
    34021 gatcgtctcg ttctacatca ggactttcat tcaatttgaa ttctttttcc atctcatcta
    34081 actttttaaa gtcattgcca gattcttttg ctaatttcgc taatttatct tcggtttctt
    34141 tagccataaa gtaacgcccc ccttttgatt tcagcttagc atatcacact gaaaaaacaa
    34201 aagaaaacgc taaccaacga tcatttttag ctatttctca tctttaaaat caagcatttt
    34261 ttgccggtat aaaatcgtag ggagaactgt tttccatacc aatcatcgga tctactaact
    34321 ggttatcgat cacttctgct gtaagaacga aatctttaga aacagcaggt tctacctctt
    34381 ctttcttttc ttctccaaca aatgaagaaa acagatctgg ttccactgtt tccattactt
    34441 ggtttttcgt ttcattcgtc ccactgtcat cttctacagg tagcttttct tttacaaccc
    34501 gtggctcttt tgtatagttc ccatcttcaa atggatcatt tccgaccaaa gaatcttcat
    34561 aagctgctag tttggtccgg agagtgatat tcattttatc attatccatg atccttgatt
    34621 tcaacgtagt catccggctt gctgattcat tcgctacaca agtgacatag gcttgctctt
    34681 cccctaacaa aatcaacagt tccttactgc gagtcactgc cgtgtagagc aaattacgct
    34741 gaagcatccg ctgatattga tggaccatgg gcaaaataac catcttgaat tcactgcctt
    34801 gtgatttatg gattgagcaa caataagaaa gagtgatttt attccattcg tttcttttat
    34861 aagtgacctc atttgcatca aactggataa ctaattcatc cactttatct tcagaatctt
    34921 ttgccaagat gatacccgtg atttctccca tatctccatt gaatacattc aattcgggag
    34981 tattgactaa ttggaggact ttgtctccta tccgataaac ggtgtcgttc caatgtactt
    35041 ccttttttcc aggtttccct ggattgaata tctcctgcat cattttattt aacgcatcga
    35101 tccctgcagg tccccgatac attggtgcta atacttggat atcttgtgca cggaatccct
    35161 tatttttcgc tttttccaca attttagaga tcaagggttc aatttgataa gcatcacttc
    35221 ggaaatagga acgatctttt tgattctttg tgaaatcttt tggcagacgt ccttctttga
    35281 tctcatgggc aagaggaata atactagaac catcaccttg ccgataaatc tgattgagtt
    35341 ccattttggg aatttgttcg atttccaaaa gatcatggag tacttgacca ggaccgacag
    35401 agggaagctg atctttgtct cctactaaga taacttgcat attcgtcgga atcgctttga
    35461 ataatgtgtt agccagccat gtatcgacca tagacatctc atcaacaatc aaaagtccgc
    35521 cttctaattc tttcgtactg actgaaggtg ttttttcccg tccattcagc cccagaagac
    35581 gatggatcgt actggcaggc agacctgtcg tttcgttcat tcgtttagct gctcggccag
    35641 ttggtgctgc aagtagaata ggaaaaattt cttgggtata ttttgaagga tccaattcca
    35701 gtccgttcaa ctctgcaaaa agattgacga ttccgttgat cacagtagtt ttccccgtac
    35761 ctggtcctcc tgttaaaata aatagagggg aacggattgc ttcagcgata gcttgttctt
    35821 gcgaaggtcc atactgtatg ccgaatattt tttcgatacg ccgaatattc ttctgcactt
    35881 ctttttcagg ataatggatt tcttttttac gactgagaag gcgctggatc gaattaccga
    35941 tcccccattc agaaaagtac aaactgtttt cgtaaagatt agtctcttct tgttgtatct
    36001 ttccttcttc aacaagttgg ataattacag tagcaacctg atcaggctgg atttcaattg
    36061 ggcgacttgt ttctaaaata cggatcgttt gctgaagcaa tgtctccgcc ggtacgtatg
    36121 tatctcctgt ttgaactgca tgctgaaaaa tctgatgcaa gatagctgct cgaattcttc
    36181 tcggtgaatc tgctgcaata ccgatttgtt cggctagatt atctgcacgt ttgaacccga
    36241 ttccttctat gtcttcgact agctggtacg gattttcttc gatgatatcc aaagactcgt
    36301 tcttataggc ctggtagata ctgaaagcta actgactacc gaacccgtaa cgattcagtc
    36361 ccatgatcac ttgatccatc ccgtggttta gccgaatcgt ctcagcaagc atttctcttt
    36421 ttggcttcgt caaaccggag atttgtttta ataccgaagg atcagcaatg attttttcga
    36481 tagcatcttc tcccagcaat tctacaattt tttctgctgt tttcttcccg attcctggga
    36541 atttttcgct ggaaagatac gctacgatcc catttgcagt tgttggcttt tcttgctcat
    36601 atctttcgag tttaagctgc cttccgtatt taggatgatc cactaattct ccataaaaac
    36661 gataagtctc attttcttga atatcgccga aacttccggt gactacaatc tctttttcac
    36721 ggtaatcagc tgtggtttca ctgatgtgta ccaaaagcac tttgtaaaaa ttactaggat
    36781 tttgaaaaaa aatcgcggca actgtaccaa cgaaataagg catttcttct ggctgcatcg
    36841 gaagccgcct ccttcttcta tagtaaaggt tctggtgctg tttcagttcc taaaaaatct
    36901 gtttggttcc atactttcag ttcatatggc aataatctgc aaggctcttc agtctctaaa
    36961 atcgtcaaac tactattgaa caatccaccc atttcacgca actcacttag ctcttttcct
    37021 gtcatccatt gaatggctgc agttaaagaa gctccatgtc ctacgaataa aagtgggcca
    37081 tctttatgtt gggcaaccgc atctgctaca acagtttcga tacgtgtgat tgccgattca
    37141 atcgtttctc cgtgaaaagg agaaggatca tacagatcta aatgattacg caaattcggc
    37201 agattatccg gatatttctg gtacatttca tcgatcgact gaccttctaa agtacccagc
    37261 cctagttctc ttagcttatc tgtataaaaa atctctaccg gacgagcaag ctctttgttg
    37321 ataccttctg ctgtatcacg tgctcgcttt gctgtactgg aataaattgc tttaaaagga
    37381 acatatttta cctttttccc aaatgctttg atttcttcgt aactttgtgg caaaagcgga
    37441 gaatcgccat tgctcccttg aaaacgtctt tcctgattcc attgtgtctt cccatgtcgg
    37501 gtaaaatata gttccatacg cagcaaactc ctgtctttct tttattttac tctataataa
    37561 actgtctaat ccagcttgtc attttcatag tgatgcgtca attctaaatt agggaatcct
    37621 tgctggcgaa gtgcttcata cactatcatt gcagctgtat tcgataaatt caatgagcgg
    37681 acatgctcat cattcatagg gatgcgtaaa cacttttctt cgttttcacg cataaatgtt
    37741 tctggcaaac cggttgtttc ttttccaaat aaaaagaagt gctcttctcc atcagcaaaa
    37801 ttttcgtcac tatatacttt atgggcaaat tttgagatca aatgtagttt acgattacct
    37861 aaaaatgtta aaaaagcttc taaattactg tggtaagtaa tatttacatc attccaataa
    37921 tccaaccctg cccgttttag atgtttgtca tcagttgaaa aacctagtgg ctcgattaaa
    37981 tgaagcggag tattcgtggc tgcacacgta cgggcaatat ttccagtgtt tgcagggatt
    38041 aatggttcaa ataaaacgat atgattcatc aggatcattc ctctcatatt tatgttcgtt
    38101 tctattttct gacaatttga tttttgcata aaaaaaaacg tatcaactcg gtgtcagtca
    38161 ctgcgagtcg ttacgttagt gaagctcttc tcactaactt ttatcagaac aggaacctga
    38221 taaaaaccaa tgaaaaaaca acttgttgct aatataacac cctcaacttt tttttgcaac
    38281 ctctactcat taaaaactgc tagaaaaacg ttttctctta gttcctttta ataattctat
    38341 gtcaattgta atcttcttca aaggattctt atataagtct gctttgattc gctcttctac
    38401 ctgccattct aaaggtgaca tggccaaaag atctttttga ttttcctctg gtatctcaaa
    38461 ttcataggtt acacgctgac gttcagttaa tttcattgct tcagcaaact tggctactac
    38521 acgttcattg gaataactct gtttttcagg ttgattcgga taaaaagcct gacgtagttc
    38581 ctttaaataa ttttcttgtg gcaccacctt caacacttct ccatcttctg agagtacacg
    38641 ttggaattct tggtaatgag aaggcgaaaa gatatttaaa accgcgttga atgtttgatc
    38701 agcaaacggc aaattggtca aatcagctac acaccaaaat gcttcggcag attgttctgt
    38761 agctgcgtag actccttctt ttgaaatatc aaatccaaca gcattttgaa tctttttcaa
    38821 ttttgaaagt tcattcaaga agctaccttc tccgcagcca acatctaata gcgagacttc
    38881 ttcaggtaaa tgctgcgcaa tagtctcaat aacgggctga tacatgccag attggatcat
    38941 tctacgcctt ggaacgaaca ttccttgatc gtattctgtt ttaaccgcat gggataaaaa
    39001 ataaagtgtt ccttttttgg aaaggtcaaa acggtgatta tttttgcata tcaagccatt
    39061 atcagacttt tttacttctt cttggcaaac cggacagcgg aacagctgat gattttctgc
    39121 caaaaactgg cgtcctcggt caattttttt taacatagtt ccccctcatt tctgttttat
    39181 cttaccgtta gatgctcact ttggcaactg ctaaaaatat gatacaatgc ctagaagaaa
    39241 gtgaaagata aaggagaaga ttagatgatc aaagaatttt gcgctgaaaa ctacacatct
    39301 attcctctag caattgctag aggagccgat cggatcgaat tgtgtgacaa cttagcggtc
    39361 ggtggcacaa ccccgagtac tggtgtgatc gaagaaacgt taagttatgc tggggaaaaa
    39421 gatatccccg taatgacgat tattcgtcct agagggggcg acttcgtcta taacgatatc
    39481 gaattgaaaa tcatgcatac tgatttgatt gaagcaaaga aattagggac ggatggcgta
    39541 gtatttggtt gtcttactcc ttcaggctgg ctagacgaag aggccttaga actattgatc
    39601 gaaacagcag aagggttgca gatcaccttc catatggctt ttgacagtat tcctgaagat
    39661 cggcaattcg aagcaatcga ttggctggta gaacatggtg ttgaccgtat cttgactcat
    39721 ggcggtcctg ctgacaaaac tattgtagaa aatttcccac ggcttaaaga attgattgcc
    39781 tatacagatg gccgcttaat cattttgcct ggtggtggtg tcactgcaga gaatgcagca
    39841 catgtagcta aagaattgaa cgtaagcgaa gtacacggaa ccaaaatcgt ggatcttcag
    39901 ttataatctt ataaacaaaa aaacatttgc aacaggcata atgcttattg caaatgtttt
    39961 tgttttttta cataaaatag cccataaata aagaagcaat attgaaataa atcagtacac
    40021 tcgtcaaatc gctcaaagtc gtgataaagg gaccgcttgc tactgctgga tcaaatccta
    40081 gacgatccat tagcatcggg attaaactac ctgcaagatt cgccactgtg attgcaaata
    40141 gcatagccat ccctatgaca aaccccaaga taaaattatg tttccaaaaa cctacgatta
    40201 gaaaaatcgt gatccccgta acagctcctg tgacaacgcc ggtcaagact tcagccaaaa
    40261 caagtcgaac aaatccatga tctttctcat cagaaatcgc aagtcgacga acagccacag
    40321 ctagcgactg ggttccagca ttaccggcag ttcctgtaat cagcgaaatg aagactgcta
    40381 aaatactcgc ttcacttacc aagtcttcat agtgactgat taaagaagcg gtcgacatac
    40441 ctaaaaacaa cagagtgatc agccaaggca aacgtcgaga cgctgccttt actggatttt
    40501 cattaatttc ttccacattg accccggcaa gtcctgaata atcgctagca gcttcatcat
    40561 caataacgtc gatgatatca tcgactgtga cgattcctaa caaatgatca tcataatcgg
    40621 taacaggaag agctaagaag tcataatctc taaatgtttg tgccacatct tcttggtcgt
    40681 cgcctacatg aactgaaagc actcgttcgc tcatcagatc agaaatcatt gcgtcgtcat
    40741 cattcacgat taggtcgcgc agagaaataa cccctactag ctgaccttct tgattaacga
    40801 catagatgta atagattgtt tcagctacat ctgcttcgtt cttcagcacg tacatcgcag
    40861 agcgaacagt ttgattggct acgatcgaaa caaattcagt tgtcatgatc gcgccagccg
    40921 tttcatcctc gtaatggaga agttccttga tttcactggc atcgtctgcc gacatcaaac
    40981 ttaaatattt agcaatttgc tttttatcca gcgtattaag caagtcaaca gcattatctg
    41041 tatacatctc agaaagcata tcagctgcat agctaggacg catttcatct aggtattctt
    41101 tcatatgttc gttgtcttct tcaatcacat cgaacatatc cgctaattct ttaggcgaca
    41161 gataggaata gatcagctgc ctgttctctt ctgtaagaga ttggtagaat tgcccctgct
    41221 catagatatg catctctaaa aaactgtttc tgaatgcctg aatattattt tcccttaaat
    41281 cttgagtcaa ctgggcaaat ctttcctcta attcttgatt ttcatccaaa ccaatctcac
    41341 ctcgccttat tcttcaaacc gtctcacaac tgcctccatg tctgcggcta aaggctgctt
    41401 gaactccagc aactcttctg taaatggatg gtaaaaacgc agatcataac aatgaagggc
    41461 ttgtcttttt attcccagat ccattcgccc gccatacata tcatcaccga gcagggcaca
    41521 gccaattgcg gaaaaatgaa ctcttatttg atgtgtcctt cctgtatgca gctggatatc
    41581 aactaacgcg atttctgtta gtcgcttctc tatccagtac tcagtctgtg ccgaacgtcc
    41641 ttcatcagag accattcgct tcaatagcga agaataatct cgggcgatcg gcaaatcgat
    41701 atgtccatgt gccgataaat ctgaaagcct tccactgact aaggcctgat attttttcac
    41761 aaacttcttt tcacgaagtt gcatatctaa ttttgcgtgc gcaaatccat gctttgcaaa
    41821 aagcatcaaa ccagatgtat cccgatctag tcgcgtcacc acatggatta cttggttttc
    41881 gtaaccttgt tttttataat aagcctttac acgattagcc attgtcccat ttggatggta
    41941 ttgggcagga atagacgaaa cgcctgctgg tttatttact accagaacgt gttcatcttc
    42001 atacacgata tcgatcggtg tttcatccaa aagtacggtt tcatgttctc cttcagcagg
    42061 aatcacaatg gttaccttat catccttagc tagagaaaac aagacatttt ctacttgatc
    42121 attaactttg atttgaccgc cttgaaattt tattttcgct aacagaccct ttgagatccc
    42181 tttttctttt aaaaaatatt ttacttgctg agaatgatct ttctcataat cccatgtaaa
    42241 ctgcatatca atcctcacta ataaaggcat ctttcacacg atgccaaaaa tgcatatgtc
    42301 gataagaagc aaaatggatc cgttcttctg ctattcgata ataaattgag cggatatttg
    42361 cttttgcgac attcaattga tcaaccgtta caagataatc ttctgtattt tccaatttta
    42421 tttctagcca ttcatcatgg gcaatcacga tcggagaacc caaagtacga aacactcgat
    42481 tattcagtga agcaatctct gccaactgaa aggcattgat actaggatgg atcacagcac
    42541 cgccgacact tttattatat gctgtcgaac cagtaggcgt agaaatcgtc aatccatctc
    42601 ctctaaaact ttcaaacagc tcgtttttga tgtatacatc ggctaccatc gtacgattgg
    42661 cccgtttaat cgttgattca ttcagtgcta aaaaatgctg gtccggtgtt tcatccaaat
    42721 aactgatccg aacatccagc aagggataac taacactttg ttcccggttc gttcgtaagc
    42781 tgtctactag ttcttccagc tcgtaatccc gccaatccgt gtaaaaccct aaatgacctg
    42841 tatgcacgcc caagaaacgc acatcattca gacaatgact gtagtgatgg aacgccgaca
    42901 ataatgtgcc gtctccgccg actgagatga ccaattctgg ctcatgatca tctcgttgaa
    42961 tccctgcttg ttctaaaagt gcgttcaaac gttctgtcac ttctatagtc tttgcttctt
    43021 tattatgaac gatcgctact ttcatcccct gcctcctctc tttcgctttc tttcttcaaa
    43081 agcgttcgat agtagacatc atctgatttt cctcttccat gggagaataa ccgctgtgct
    43141 tcttggattt cttcacggat actggacatc tcttcatcca actgataggc agcttctgcc
    43201 gcacgctgaa gtcgttcact catttcttct ggaaaaacgc cttgatattt ataattcagc
    43261 gaatgctcaa tcgttgccca gaaattcatc gcaagagtcc gaatctggat ttccgccaaa
    43321 attttcttct caccgctgat cagctgtaca ggatactcga tcaccagatg ataagaacgg
    43381 tagccacttg cttttttatt tgtgatgtaa tctcgttctt ggagaacttt catgtctttt
    43441 cttttacgga tgatctcaac aacttgatgg atatcttcta caaactgaca catgatcctt
    43501 aacccggcaa tatcagacat ttcttcttct aaacgtccca ttggaatatg ccgtaattgt
    43561 gattttgtta caatactatc gactggcttg actctcccag tgacaaattc aatcgggaca
    43621 tgtcgatttt gttcacggaa ctgtttgcgg atcccccgca gtttcacttt caattctgat
    43681 acagtttgtt catatggtgc taaaaatgct tcccaatctc gttccatagc tgatacctct
    43741 acttcattaa cattcattgt ttattctacc atatatgcaa agttttttcg tactcttgat
    43801 ttatgcattg accaaaaaaa gaaaattagg caaaatagac aaaaaggagt cgatcaaatg
    43861 acacaaatgc ttgaaatcga atataaatct atgctgacaa agcaagagta cgagatattc
    43921 atcgctcact atcaattaac agataaagat ttccgtgtac agactaatat ttatttcgat
    43981 accgttgatg aacagctgaa gaaacaaaat tgtggcctac ggatccgttt cgttggtcat
    44041 caagctgaat atacattaaa aacacctgct gaaaaagggc gtctcgaaac aacggatacc
    44101 tttataacag aagaagcaga agcatttatc ttcgaaaaac gtctgcctcg ttcaggcgca
    44161 gtttttcaaa aactttctga tttgaatatc gatcctgctt cattaatcca aaccggaaag
    44221 cttacaacaa aacgggctga attccctatt gaagaaggac ttttagctat tgatgaaagc
    44281 tggggagaca atctacacga ttttgaactg gaactagagg taaggggatg catctgtggg
    44341 aaaaattgct tttataaatt ttttaaaacg tttttcttta ccttatcgtc ctgctaagaa
    44401 caagatccaa cgtatgcttg aagctaaaaa ttagaaacct ttcattttca caaggcactt
    44461 gagttcattt ttttactttt ttctgttaag ttataggatg tgaaagaatc tagtcactag
    44521 ttgtatagaa tctattttgg gggagattca tatgatcgaa atctatttat ttgttaatcc
    44581 tttaggggaa tactgtttcg aaatggaaca acagttatta caatttatcg aagaagagta
    44641 cgggacacca tcaaaagaaa agatgcaatt tcgctttctt cctttggtca atttgcagac
    44701 aattggggat gtcatgcaac aaaaaggaat ttctcagaac aatcttgagg aacgtaatca
    44761 cttattctca atgacttatt ctgcagcatt ggactgcaaa gcggcgcagt ttcaaggaaa
    44821 gaaaaaaggc cgccgttttt tgatcaaatt gcaagaagca gtcggctgta atgacatacc
    44881 atattctcaa gaacttgctg aatccatatt tgaagaaatc ggcggcgatt tgaatatgtt
    44941 taaagaagat cgtcagtctg acttcgttaa agaagtattc ttttctgatc aaaaaattgc
    45001 tcgggaaatg ggcatcacaa aacacccttc tgcagtcgtt tacaattatg cctgcgaccg
    45061 tgattttggt gttttagtag aagtaaaagg tacaagtagc ctttggagaa tcacagagct
    45121 gtgtaaaaca acagataact catatcaagt ttttaatggt gaacatttac atacaaatga
    45181 ttctcagttc cattcaagaa ctagccattt acacttgctt tctaattaaa aaaatacgtc
    45241 gactccctta caagagagcc gacgtatttt tttcgtttct attcgagaaa atcaaaaata
    45301 gaaagcctta taaacgatcg atcaattctt ctaattcatt tagacgttgt tcaaacatcg
    45361 acattgcatc ttcaatatat gcagcctgtg tcatatcaac tcctgctttt ttcattactt
    45421 ccacaggata atcgctgtta cctgctttta ggtaagccaa gtagttctct agtgcctctg
    45481 gttcctggtt tagtattttc ttcgccagtg ctgaagcagc agaaaagcca gtcgagtatt
    45541 ggaaaacata gtaattataa taaaaatgcg gtatccgtga ccattcgaat ttaatttctg
    45601 ggtcttcttc gactgctgga ccataatatt ttgcattcaa tttaccataa ctatcactta
    45661 ggtattcact agtcaatggc acaccttttt catcttccgt atgcatgaaa tgctcgaatt
    45721 cagcgaattg tgtctggcgg aaaactgttc ctttaaaccc atccaagtaa tggttgagta
    45781 cataagcccg tacacgaggg tctttttctg tttccaataa atactccgtt aggatatttt
    45841 cattcgttgt cgaagcaatt tctgctaaaa agatggagta gtcgccgtac acataaggct
    45901 ggttcgaacg agtgaaataa ctatgaacac tatgtcccat ttcgtggaca agcgtaaata
    45961 gctgatccag cgtatcatgc caattcaata aaatatatgg atttgtgtca tagcttcccg
    46021 aagaataagc accgcttcgt ttccctttat tttcgacaac atcgatccaa cgttcagaga
    46081 atgctttttc tacgatggcc atgtattctt cacccattgg tttcagtgct tctaaagctt
    46141 tttcttttgc ttcttcgtac gtaaaggtaa ttggagcttc ccctaagacc ggtgtataaa
    46201 gatcatacat gtgcagtttt tccacttcta ataaacgttt ccgtaattcc atgtatcgat
    46261 gtaacaaagg caaatgcttg tttaccacgt ctaccaaagt atcgtataca ctttcaggaa
    46321 tatgattatt gctcaaagac gcttctctgg cagagctgta attacggact ttcgctttaa
    46381 aattatgtcc ttttatatgt gtgcctaaag tagaagcaaa tgtatttcta aattgttcgt
    46441 aaacactgta caatccctta aatgctgctt cgcgcaccct tcgatccgtg ctttctagca
    46501 actgaccata cacgccatga gataattgga ctatttcacc attttctcct tcaatcgttg
    46561 gaaaaactag atctgcatta ttcaaaacag caaatgtatc acttgaagca tcaaagattt
    46621 cacctgctcc agcaagaaga gattcctgct cagcagataa gacatgggct cgattatcta
    46681 ctatttgctg gatataatgg cgatagactt ccaattttgg ttcttctttg aaatattgcc
    46741 aaatctggtc atctgacaat tgcaatattt ctggttcaaa ccaggaaacg gcttcactaa
    46801 ctttcgaaaa cagactgctt gctcttgcat aaagcgcttg gtaatctgta tttccagtgt
    46861 cttgatcgtt tttaaggtgc gcatatacat aaatgacttc agtttggcga taaaccctca
    46921 atacgaattc aatcgcattt aaaaattgag aagcgccttg atcaagtgtt cctttgtgtt
    46981 tttcagattg ttttaactct tctgataatt ccaaatattt ctcatcaaac tcttggtcgc
    47041 ttgaaaagat cttggtcaag tcccaagtta aattttcagg caattcttct cgttttggca
    47101 actgctttac ttccatgaaa tccactcctt ttcttccctt agctctatct atcaaaatag
    47161 atcatagtct aacatccaca tgtcacagca tctaactttt ctactcattc atgtgtctgc
    47221 ttccatcaat tttagcataa ctaaatcttg ttttacatag aaaaccgatt cctagcctat
    47281 ttttatactt actatttttt caaagaagac aagtgtatgc attcgttaaa aaactgctga
    47341 tagaccatct cttcatcaat cataggaaaa agccattccc ttttatgttc ttttatttgg
    47401 caaagccatg agttgaaatc aggaactttc tggttttcca aggcttcttc gtacaacatt
    47461 cgataaagaa aaacctgttc ttgtaaataa aagaaaaacc gacttttttg atagatccac
    47521 gatgatagat gcaaaagatg tttatgacgc aagtagcatt gttcttgtat ggatactgta
    47581 ttcttttgct tgtgaataag ttggcgtgtc agccatttct tcccatcttc actcactttt
    47641 tctatttttt tcatttggtt cattttaatt tcgttactag tgaatatttc tttaaggtcc
    47701 ctactagaaa aagtccagca gctactgcaa aaggaaatat gccctgatac agtctctgta
    47761 atatgatgat acaaataaag cttttgtttc ttgagatcag ctttccagca atgcactcct
    47821 ctttttctat tatagtagca gcaacttttt tctatcaatg agaattgcga ttgtccaaga
    47881 aaagaattac caagtatcca ccatacttgg taactgttat tcaaataatt ttgggttctt
    47941 tcaataaatc tctggtgaga cagtctagaa cattggattt ctattgctag attcgccaac
    48001 aaaatatccg gcctttgccg caacctcggc aaataggctt ccaaaaatac tggttctact
    48061 gactgtccca gccaatccat gaagcattcc tttaactgta agtgctcttt tgattctctt
    48121 tcactaaaga ccgaacagtt atctgatttt tgatgggcaa aatgagcaca ttttttcttt
    48181 ccctttttca aaatcactct ttttcgacag ccacgacaat aatatttctt tcctttttct
    48241 gcatgtttag caaaacagca cttattatat tcattcattg caagcagcat ttttctccct
    48301 ctgaacttat ttacgtctaa gaatttattt acacgaaaaa agagggcgtg aaaaagctat
    48361 tctgcatcaa gtaataagaa cagctaaaca aaaatagttc ccaatatttt tcgtttggtt
    48421 gggcttgtta ccgcagatgc aagcttttga acacatttat acgaaaaaaa gactgcgaca
    48481 caatttttgt cacagtctct tactataatg ctgatttata caaaataaaa tttcgttcgc
    48541 tcgatagcat tacgcgccat cagacattgt ccatattcat tcaatgtttc tggtgtacga
    48601 tcgctctttt cagcaaactc caaaattcga gcaatctgat tctctacatc cgcttggttg
    48661 agattttcca tccagaagtg aacgactagg taatagcgct cttcgaataa atacaaatca
    48721 gtccaagcag actctagttc gacatctgtt gcaagttgga tcacttgttc aaaatcttca
    48781 aaagcaaaag ttgcttgtct ttcctgttct tctagggcgc ttccagataa ttcttcctcc
    48841 ggatcgctgc caataatctg ctgacgaaga aagtcactca catcttctga atcaacagta
    48901 gaatcttctg attgagaaat ttcttcatcc ggaagatttt tactgataaa cagttctaat
    48961 ccgtctcctt ttggcaaaac ttggaaagtg acagcttcgc ttcctcggaa ctcctcttcg
    49021 atatcaactt cttccaaaat actataaaag aaattttcga tttctttgtg attgccaagt
    49081 aaatccaaaa atgaaacgcc ccgttcctct aaatcttcat gaccaattag tacacggatc
    49141 gtattttcat taatatgttc catttccatt ttgctacacc tcactttgcc acaatgttta
    49201 atatagattc attgtagcga aatcagctta aatgtaaaga aaaaaaatct cgctgttaag
    49261 cgaatttaat aaaaccagcg gatttttcaa cctttcaagc tatcttattt aactataaca
    49321 tttttggcag acgaatacta tatagccgac ttttcagcta attttccctc tctatttttc
    49381 gtgcaatcat catcttctac taccttttta tacatctaat caagaatact ttttgtaaaa
    49441 acaaaaaaac ttggaaaaac tatctttgta atagtctttt ggctctatac tttttgatgt
    49501 tggtgaactt caccaacatc ttttttgtat aaaaaaatat agaaaagaca ccctgaaatc
    49561 ctttagaatt aatttggcta ccaattcata ggaggattca agatgtctca tactcagctt
    49621 atcaaagata ccctaaatat tctagaccta aatatctatt tcgaggaaaa ttgtttaact
    49681 aaagaaaaat acaaagggca aatttgcttg atttacaaag gaactcttct gtacaagcca
    49741 gaagagtgtt cccattgtct ttgtgtcgtc ccctctagga tcattcgttg ggggacgaca
    49801 accgttcggt tgttactgaa tgatgtgtct gagtatcgca cttatctgga gctgaagaaa
    49861 caacgattta aatgtaagtc ttgccaaaga acattcgtag cggatacctc tgttgctgaa
    49921 aaacactgtt tcattagtca aaaagttcgt tggtctgtca ttgctcgctt aaaagagaat
    49981 acttccatga cggaaatcgc tcgtcagaaa aatatttcca cttcttcggt ctatcgtgtc
    50041 atgaagcgat tctaccgccc attgaatcct ttcaaacaga ctttgccaaa ggtcttatgc
    50101 tttgatgaat tcaaatcggt tcgaagtgtt gccagtgcaa tgagttttat catgatggac
    50161 ggacaaagcc atgcattatt ggatattgtc gaaaatcgac gcttgcctta tttagaacgc
    50221 tatttttctc ggttttcatt agcgactcgt gaagccgttc aattgattgt tattgatatg
    50281 tatgcgcctt atgtttcgtt ggtgaaaaag cttttcccga atgctcagtt gatcatcgat
    50341 cgtttccaca tcgtccaaca tattggcaga actttcttga atcatcgagt gaaagaaacc
    50401 acggtacgac ttaaaggaaa ttcccactct caacgagggt tagggaaaaa attgaagcgc
    50461 tattggaaac tcctacaaaa agaagaagca aagttaaact atgaaaaacg ggtttggcgt
    50521 gccagtttta aagattattt gactgaaagt gagattgccg atcgactgtt agctgcttgt
    50581 cccaacttac gccaaggcta tcagttatac caagacattc tttatgcggt aaagaaaaga
    50641 gatcagtcac tatttgagga ttgtttaact agagacatta gcggtctgcc agaaacctat
    50701 acaaccactt tacgaacgtt taaaaagttt ctgccacaga tc
//
LOCUS       DAZKLM010000008        48033 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3764
            SAMEA115706913-rid22582563.denovo.008, whole genome shotgun
            sequence.
ACCESSION   DAZKLM010000008 DAZKLM010000000
VERSION     DAZKLM010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706913
            Sequence Read Archive: ERR13196647
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 48033)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48033)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287823.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287823.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,004
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,849
            CDSs (with protein)               :: 2,849
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 41 of 96
            Pseudo Genes (incomplete)         :: 52 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 15 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48033
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706913-rid22582563.denovo.008"
                     /isolate="VRE3764"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            261..632
                     /locus_tag="ACG842_000408"
     CDS             261..632
                     /locus_tag="ACG842_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/Cl family transcriptional regulator"
                     /protein_id="HFQ0246973.1"
                     /translation="MRDYVTDKTQEVINQSQSLNELSDKAKEEAVAIISSGISDYIDM
                     SGEINYGDKDKIVRMIKAFIKTFDVKADLKFNDTYLVGKLINILGTEKDTETFISSLS
                     MLFTDKHFSTQLGGEELVEIF"
     gene            complement(906..1598)
                     /locus_tag="ACG842_000409"
     CDS             complement(906..1598)
                     /locus_tag="ACG842_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFQ0246974.1"
                     /translation="MKGIIFDFNGTMFLDSHLHEAAWLHMIHDHTKDGLSDEDILKNI
                     HGRTNTEILKHFISEHLTASEIAVLSEEKESYYRMLCLQNENELQLTKGLEATLDQLT
                     DQQIPLTIATATVKENVAFYFDIFDLARWFDWDKIVYDDGSFPGKPQPDIFLKAADKL
                     SLAPADCLVIEDAYSGLLAAKRAGIGTIIAIDPFGKNKTVFEQAQLNKDGTIKDFTGF
                     WTEHLSATSVNS"
     gene            complement(1601..2386)
                     /locus_tag="ACG842_000410"
     CDS             complement(1601..2386)
                     /locus_tag="ACG842_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFQ0246975.1"
                     /translation="MLKKERLLTIVEMVNKKGILTVNEIINQLDVSDMTVRRDLDELE
                     KSGKLLRVHGGAQSLSYTLDQELSHTEKATLQIQEKKEIAATAASLIHEGETIFLGPG
                     TTIELLATHLLNKKIRIITNNYPVFDILAQADGPEILLIGGDFRKNTGAFVGPITNDN
                     LRRLKFTKAFISSNGVHNEAISTYSTEEGEAQQIALNNSRKKYLLVDNKKFNRDDFYV
                     FYNLHDFDLLITDRQISREVQEHYAQYVDLQIADSTENTTIGA"
     gene            2623..4023
                     /gene="lacG"
                     /locus_tag="ACG842_000411"
     CDS             2623..4023
                     /gene="lacG"
                     /locus_tag="ACG842_000411"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033920 - 6-phospho-beta-galactosidase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HFQ0246976.1"
                     /translation="MLKLPEDFIFGGATAAYQVEGATKEGGKGAVAWDDFLEEQGRFS
                     PDPASDFYHQYAKDIELCERFGVNGLRLSIAWSRIFPDGAGKPNPEGIAFYHRVFEEC
                     KKRNVTPFVTLHHFDTPKRLFDHGDFLNRETIEAFVSYAIFCFHEFKEVKVWSTFNEI
                     YPVATNQYLLGVFPPGIKYDFTKIVACLHNMMVAHARVVNYFKENELPGEIGVVHSLE
                     TKYAATDAPEDKHAAFLDDALSIRFLLDATYLGYYSTETLTALDEICEANQASYHFPE
                     EDFVELKKASTRNDYLGINHYQCHFVKAYDGENAIHHNGTGEKGTSVYKVKGIGERIY
                     KEGIPRTDWDWLIYPEGLYDLLLRIKSDYPHYNKIYITENGMGYKDQFEDGIIMDQPR
                     IDYLRVYLESLSKAITAGVNVKGYFLWSLMDLFSWTNGYNKRYGLFYVDFETQKRYPK
                     ESAYWYKLVSETKTII"
     gene            4048..4365
                     /locus_tag="ACG842_000412"
     CDS             4048..4365
                     /locus_tag="ACG842_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HFQ0246977.1"
                     /translation="MNKEELQMLGFEIVAYSGDARSTLLKLLKEVRQGNFEHVDSALK
                     EADENLTLAHNSQTKILAEEASGKEMEMGFIFIHGQDHLMTTLLLRDLIQDFIVLYRQ
                     NQG"
     gene            4378..6051
                     /locus_tag="ACG842_000413"
     CDS             4378..6051
                     /locus_tag="ACG842_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose transporter subunit IIBC"
                     /protein_id="HFQ0246978.1"
                     /translation="MDGIIKQIEKGKPFFEKISRNSYLGAVRDGFLTAMPAILFSSIF
                     ILIAAVPEIFGYQWPESVSTWLWKVYNYSMGVVGLLVSATTARCLADSMNRRLPKNKV
                     INTTSVMLASVVSFLLLSVSPIEGGFSTEYMGTKGLLASFVAAFLTVNLYKFCVLKDI
                     TIKMPKEVPGTISQTFRDIFPFSFSVFAAVLIDLGIRQIFQISFAEAIITVLQPLFTA
                     ADGYLGIAIIWGAMALFWFVGVHGPSIVEPAIAAIIYANVETNLQLFNSGEQASKVLT
                     VGLGNFVGTMGGTGATLVVPFIFLLFAKSKQLKAVGKASFIPVSFAVNEPLLFAAPMI
                     LNPYFFIPFLLTPVVNVCLFKFFVDVLGMNSFMYVLPWATPAPIGLVLGTGMGILAFV
                     LAAVLIVVDFLIYLPFCKAYDAVLVEKEAQKAEVIATEEGASADVREEVPVAASQVIS
                     DANKEINVLVLCAGAGTSGMLANALKKGASESKAPISAMAGAYGSHYEMLQDFDMVVL
                     APQVNSYYDDIKEDTDRLGIKLVATKGAEYIGLTRDPEKAVQFILNQFQ"
     gene            6065..7696
                     /locus_tag="ACG842_000414"
     CDS             6065..7696
                     /locus_tag="ACG842_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinogalactan endo-beta-1,4-galactanase"
                     /protein_id="HFQ0246979.1"
                     /translation="MKMGIWLRKAVLFAAVLGALGAVSKTVEASASEFIRGADISILS
                     DMEKSGAKYYEDDISKDALEILKNNGVNYVRLRLWQDPYNSNGESYGAGTNDLETTID
                     LAKRAKNLGLKVLLDFHYSDFWVDPGKQNLPKAWQGLTFEEMNTALYDYTKNVLSEMK
                     QLDVYPDMVQIGNELNSGMLWPYGKSWGEGGGEFDRLAAFLKSGIQAVRDTQPKTTPV
                     MLHLADGGDTGAFTWWFDEITSRGVSFDLIGVSYYPYWHGSMNELQTNMNAISQRYGK
                     EVIVVETAYGHTTANADTMPNAFGEAEAAAGGYEPTPTGQAEYLLDLADRIQAVPNNR
                     GAGFFYWEPLWYNGNVSWATQAGMNYLSVQSTMGNEWDNQAMFDFAGNALPSLRAFKQ
                     AGAQINLVKNASFESDGPTTSPSNWQTWFQQGTAKGTIKTEAGTFFGDYKLTFWQDQA
                     YEASAYQNVSGISKGTYTLSAYVMSGGEFDTNQLYIKNYGGAELNTAVVKTSEWTKIT
                     IKDIPITTGVCEIGVYSKASGNAWTNVDFVSLVKQ"
     gene            complement(8187..9752)
                     /gene="guaA"
                     /locus_tag="ACG842_000415"
     CDS             complement(8187..9752)
                     /gene="guaA"
                     /locus_tag="ACG842_000415"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HFQ0246980.1"
                     /translation="MTNVADMTNVEKIIVLDYGSQYNQLITRRIRDLGVFSELLSHRI
                     TAEEVKAMNPKGIILSGGPNSVYDENAFGIDPEIFNLGIPVLGICYGMQLITYKLGGS
                     VEPAKNREYGKALLEVTSDQAVLFKETPKQQTVWMSHGDLVTKVPEGFETVGTSKDCP
                     IASIQDTTRNFYGIQFHAEVRHSEYGIELLRHFAFDVCHCEGNWTMDNFIDMQVEKIR
                     EQVGDKKVLLGLSGGVDSSVVGVLLQKAIGDQLTSIFVDHGLLRKGEAEQVMESLGGK
                     FGLNIIKVNARDRFLDKLKGVSDPEQKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQG
                     TLYTDVIESGTETAQTIKSHHNVGGLPEDMQFELIEPLNTLFKDEVRELGTQLGMPDS
                     IVWRQPFPGPGLGIRVLGEITEDKLEIVRESDAILREEIAAAGLDRDIWQYFTVLPGI
                     RSVGVMGDGRTYDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVY
                     DITSKPPATVEWE"
     gene            complement(9811..10734)
                     /gene="coaA"
                     /locus_tag="ACG842_000416"
     CDS             complement(9811..10734)
                     /gene="coaA"
                     /locus_tag="ACG842_000416"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HFQ0246981.1"
                     /translation="MNDPMNYYRVAREEWREFYRNGQAPLTQDELDNIKSLNDRISMQ
                     DVRDIYVPLAHLIYLYMKEFESLFLSKGLFLHKYVEVPPFIIGIAGSVAVGKSTTARL
                     LQTILSRTFKRRNVQLITTDGFLYPNRILKERGIMDRKGFPESYDMEMLIDFLNRVKS
                     GQEEIKVPVYSHDVYDIIPDKYELIQQPDILIVEGINTLQLPANQQIYVSDFFDFSVY
                     VDADPVLIEKWYLDRFGALLDTAFQNPENYYYRFAIGDREEAFKMAKDVWKNVNLKNL
                     QEYILPTRGRADIILHKTENHIIDSIFMRKY"
     gene            10930..11535
                     /locus_tag="ACG842_000417"
     CDS             10930..11535
                     /locus_tag="ACG842_000417"
                     /EC_number="2.1.1.172"
                     /EC_number="2.1.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0052916 - 23S rRNA
                     (guanine(1835)-N(2))-methyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFQ0246982.1"
                     /translation="MTNHYYSERPDTAHDFEQWAFELKGRTFQFVTDSGVFSRETVDY
                     GSRVLIDAFDWEELPDEGKILDVGCGYGPIGLALAFATQRFVEMVDINERAVSLAQGN
                     AKRNQIEHVDIHQSNIYEAVHEETYAAIVSNPPIRAGKKVVHEILTGAYPRLKKGGTL
                     TIVIQKKQGAPSAQKKMEETFGNAEIVTKDKGYYIIKSVKE"
     gene            11843..12859
                     /locus_tag="ACG842_000418"
     CDS             11843..12859
                     /locus_tag="ACG842_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFQ0246983.1"
                     /translation="MKLTIRDIAEMAGVSVTTVSQILNNKGSRFSDKTRKKVLSIVNE
                     HHYKPDYFASNVINRHSKTIGMIVPDVTDFFFSKLIEGVESYLNPLGYVIMLCNSRHS
                     QENEIKYLQELSHRSVDGILLATPNILPEKYALDSGFYQKMPIILIDRGLNRRDNGRL
                     IVKEYEGAYQAVSLFIENGHTKIGMLKETTGYYQLEERFNGYRHALKDHGLPFNGKFV
                     EHGELTVQGGYTASKKLLKHKDITAIFCGNDAMAIGCYQAIDELGKKIPEDISVIGFD
                     GLKLSEYMIPQLTTVQQPSFDIGFYAARFLIDTIEFPQRKVPNKVFETKLIIRESVKV
                     LTKG"
     gene            13000..14301
                     /locus_tag="ACG842_000419"
     CDS             13000..14301
                     /locus_tag="ACG842_000419"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016154 - pyrimidine-nucleoside
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0006213 - pyrimidine nucleoside metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HFQ0246984.1"
                     /translation="MRMVDLIEKKRDGNELSKEEIEYIVTNYTNGKIPDYQVSALLMA
                     IFYQDMTNEEITNLTLAIANSGDVIDLSSLEGIKVDKHSTGGVGDTTTLILAPLVASV
                     GVTVAKMSGRGLGYTGGTLDKLEAIPGFQIELSDEEFVRIVNESKVAVIGQSGNLAPA
                     DKKLYALRDVTATVDSLPLIASSIMSKKIAAGADAIVLDVTTGDGAFMKNIEDARRLA
                     KTMTSIGKLANRETVAVISDMSEPLGEAIGNSLEVVEAIETLQGNGPEDLVEMCYALG
                     SQMVVLAGKAKTTDEARTLLQEALESGKALAKFKEMIQNQGGDPAIVEHPERILTARY
                     TMELPAKQSGVVSKIVANELGIAAMMLGAGRKTKEEDIDHAVGLKLHKKIGDTVTKGE
                     SLLTIYSNDEEITSVIELLYKNIEIGESAMKPTLIHDIITE"
     gene            14347..15009
                     /gene="deoC"
                     /locus_tag="ACG842_000420"
     CDS             14347..15009
                     /gene="deoC"
                     /locus_tag="ACG842_000420"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HFQ0246985.1"
                     /translation="MELNRMIDHTILKADASKEAVMQIIEEAKKYHFYSVCINPAWVA
                     LAKEQLQGEPVAVCTVIGFPLGANTPETKAFETTDAINNGADEVDMVINIGALKSKDD
                     QQVQKDIEAVVEAAKDRALVKVIIETSLLDREEIIRACEIAKAAGADFVKTSTGFSTG
                     GAKVEDVRLMRETVGPEMGVKASGGIHNEEEAMAMIEAGATRIGTSAGVAIVSGSTGE
                     GY"
     gene            15030..15428
                     /locus_tag="ACG842_000421"
     CDS             15030..15428
                     /locus_tag="ACG842_000421"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HFQ0246986.1"
                     /translation="MAELKQEWINTAIEALDKAYVPYSHFPVGACLVTESGKIYQGIN
                     IENASFGLTNCAERTAFFKAVSEGEKTFTHLVVAGHTPDPISPCGACRQVMAEFCSPD
                     MPVTLVGDNGVTKATTVRALLPYAFTEKDL"
     gene            15494..16576
                     /locus_tag="ACG842_000422"
     CDS             15494..16576
                     /locus_tag="ACG842_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HFQ0246987.1"
                     /translation="MKKAKLFGLGAVALAAGLFLGACGNNGSTDSSGGKSSSDTTTAA
                     LITDTGGVDDRSFNQSAWEGLEKWGKDQGLSRGNDGFQYFQSSNESDYIPNIDQALNA
                     GFKTIFGIGYKLKPAIQEQATNNTGTNFVIIDDVIDGLDNVVSATFKDNEASYLAGVA
                     AAYTTETNVVGFIGGVKGEVIDRFDAGFKAGVDAGAKELGKEIKVLNQYAGDFSAPDK
                     GRSIAQGMYAQNADIIFHASGGTGNGVFQEAKSLNESGDKKVWVIGVDRDQSDEGEYT
                     LNGEKKNFTLTSTLKAVGTVVEDLAQKSADGKFPGGEHTVYGLKEDGVGLTEGQLSDE
                     AKKAVDEAKEKIISGDVKVPETPEEN"
     gene            16788..18350
                     /locus_tag="ACG842_000423"
     CDS             16788..18350
                     /locus_tag="ACG842_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0246988.1"
                     /translation="MVQENIVIEMQNITKEFGTFKANDNINLQVKAGEIHALLGENGA
                     GKSTLMNVLSGLLEPTSGKILMRGKEVKITSPTKANQLGIGMVHQHFMLVDAFTVTEN
                     IVLGSEPSRAGMLDHKKARKEIQKVSEQYGLSVNPDAYVRDISVGMEQRVEILKTLYR
                     GADVLIFDEPTAVLTPQEIDELIVIMKELVKEGKSIILITHKLDEIKAVADRCTVIRR
                     GKGIGTVNVKDVTSQQLADMMVGRTVSFKTMKKEAKPQEVVLSIENLVVKENRGLEAV
                     KNLNLEVRAGEVLGIAGIDGNGQSELIQALTGLRKAESGHIKLKGEDITNKKPRKITE
                     HGVGHVPEDRHKYGLVLDMTLSENIALQTYHQKPYSKNGMLNYSVMNEHARELIEEYD
                     VRTTNELVPAKALSGGNQQKAIIAREVDRDPDLLIVANPTRGLDVGAIEFIHKRLIEQ
                     RDKYKAVLLISFELEEILNVSDRIAVIHEGEIVGIVDPKETSENELGLLMAGYTLEEA
                     RKELEKGAGAHE"
     gene            18343..19476
                     /locus_tag="ACG842_000424"
     CDS             18343..19476
                     /locus_tag="ACG842_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0246989.1"
                     /translation="MNDRSEKFRNLLVPVLSVVLGLILGAILMAAFGFQPIQGYSSML
                     DAALGSQRGIGETLRQATPLIFTALGFSVANSAGFFNIGLSGQALCGWIVSVWVALAF
                     PDLPKVVLLPLCVILGALAGAMAAAVPGLLRAFFGTSEVIVTIMMNYILLYVSTFALH
                     EVMPESYRSSLDSSNMISQNASLRLPWLTQAFGGSRVNSGLFLAIIALIFVWLLMKKT
                     TLGFEIRSVGLNPFASEYAGMSSKRTIVLSMVISGALAGLGGVVEGLGTYQNFFVQTT
                     SLSIGFDGMAVSLLGSGSAIGILLSAILFSILKIGGLGMQTGAGVPFEIVNVSIALII
                     FFVGINFLIRFVMAKVFQGKKQEEIVATIESKPTNQNHEGGEL"
     gene            19476..20432
                     /locus_tag="ACG842_000425"
     CDS             19476..20432
                     /locus_tag="ACG842_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0246990.1"
                     /translation="MSTIELIALILTSTLVYSTPLILTSLGGTFSERSGIVNVGLEGI
                     MVMGAFSSIVFNLTMSPSLGSMTPWIAILVGGVVGILFSLIHAVATVSLRADHIISGT
                     VINLMAPALGVFLIKAWYGKGQTDNITQNLGYFSFPGLADIPVIGPIFFKNTFLPAYC
                     AILIAVLAWVIIFKTKFGLRLRSVGENPQAADTLGINVYAMRYSGVLISGLLGGMGGA
                     VFAQSISGNFSAGTIVGQGFISMAAMIFGKWNPLGAMGASLFFGFAQSLSIIGAQLPV
                     ISSIPPVLLQIAPYLLTIIVLVVFLGKASGPKANGKNYIKSK"
     gene            20531..21697
                     /gene="deoB"
                     /locus_tag="ACG842_000426"
     CDS             20531..21697
                     /gene="deoB"
                     /locus_tag="ACG842_000426"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043094 - cellular metabolic compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HFQ0246991.1"
                     /translation="MFKRVHLIVMDSVGIGEAPDADKFGDVGSDTLGHIAKEAGLNVP
                     HLEQLGLGTIRPLEGVESVKDHQGYATKLEEVSVGKDTMTGHWEIMGLNIKTPFRVFP
                     NGFPDDLIKQIEEFSGRKVVCNKPYSGTAVIDDYGEHQMKTGDLIVYTSADPVLQIAA
                     HEEIIPLEELYRICQFVRDITKDDPYMIGRIIARPYVGEPGDFTRTSNRHDYALNPFG
                     HTVLDSLKEAGKDVIAVGKINDIFNGQGITESVRTKSNMDGVDQLLKVMKNDFSGISF
                     TNLVDFDALYGHRRDVVGYAHAIEEFDLRIPELLEAMEEDDLLMITADHGNDPTFTGT
                     DHTREYVPLLVYSKKMKEQGALPQGYYSDISATIAENFSVSSTENGESFLKLLK"
     gene            21721..22539
                     /locus_tag="ACG842_000427"
     CDS             21721..22539
                     /locus_tag="ACG842_000427"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HFQ0246992.1"
                     /translation="MTKLSEMLQETTQFILDKGVKEIDFGLILGSGLGELADEIEDAV
                     MISYDEIPFFPVSTVVGHAGKLVYGSLAGKKVLAMQGRFHYYEGHSMQTVTYPVRVMA
                     GLGAHSVIVTNACGGVNESFVPGDLMLITDHINFTGENPLIGPNEDAMGPRFPDMSQA
                     YTPDYQATAKAAAEKLGLDLKEGVYMGYSGPTYETPAEIRMSRTIGADAVGMSTVPEV
                     IVAAHSGLKVLGISCITNLAAGMQANLSHEEVVETTQRVKQSFKALVKETLTLL"
     gene            22556..23263
                     /gene="deoD"
                     /locus_tag="ACG842_000428"
     CDS             22556..23263
                     /gene="deoD"
                     /locus_tag="ACG842_000428"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HFQ0246993.1"
                     /translation="MSVHIEAKEGEIADKILLPGDPLRAKYIAETFLEDPVCYNQVRG
                     MLGFTGKYKGERISVQGTGMGMPSATIYAHELIQSYGVKKLIRVGTCGALSKDVHVRD
                     LVLAQGAATSSSMIEKNFQAFHFPPISDFNLLLKAYEIAKEKGYTVHVGNVLSEDSFY
                     KDDLTETFQLAELGVLGVEMEAAALYYLGAKYHVQTLSLMTVSDHLITGEETTAAERQ
                     STFNEMIEVGLETAIAE"
     gene            complement(23561..24247)
                     /gene="gpmA"
                     /locus_tag="ACG842_000429"
     CDS             complement(23561..24247)
                     /gene="gpmA"
                     /locus_tag="ACG842_000429"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HFQ0246994.1"
                     /translation="MPKLVFSRHGLSEWNALNQFTGWADVNLAPEGIEEAKEGGRKIK
                     EAGIEFDVAYTSVLTRAIKTCNLILEYSDQLWVPQIKSWRLNERHYGKLQGLNKKETA
                     EKYGDEQVHIWRRSYDTLPPLMEETDEGSAANDRRYAMLDKRDIPGGENLKVTLERAL
                     PFWQDEIAPALLDNKTVLVAAHGNSLRALAKHIEGISDEDIMDLEIPTGQPLVYELND
                     DLTVAKKYYL"
     gene            complement(24419..25000)
                     /locus_tag="ACG842_000430"
     CDS             complement(24419..25000)
                     /locus_tag="ACG842_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFQ0246995.1"
                     /translation="MKIIGYARTTINDVDLTTQVDQLSDFGCQEIFHETYEVEQNDST
                     AVHLESVIADMQQGDALVICQLHRLGKSTRQLTELTQLFKDRGLHLISLDEGIDTREM
                     GKIYFQLMENLANMECALIKERTLVGLDEARKKGKIGGRPKIDAKTIKKIRRLYYEKK
                     ETIQFISSKCGVSVGTCYKYINLPEKEIEQLCI"
     gene            complement(25133..25816)
                     /gene="rpiA"
                     /locus_tag="ACG842_000431"
     CDS             complement(25133..25816)
                     /gene="rpiA"
                     /locus_tag="ACG842_000431"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HFQ0246996.1"
                     /translation="MNLKQLAGIEAAKFVKDGMIVGLGTGSTAYYMVEEIGRRMREEG
                     LRITGVTTSNATKEQAEKLGIPLKSIDEVPVVDLTIDGADEISADFQGIKGGGAALLF
                     EKIVATYSKETIWIVDSSKLVDKLGKFPLPVEVIPYGSQQLLHIFEEKRFQPVLRTDE
                     NGEVLTTDGGHYIIDLHLEVIEQPESLATYLDELVGVVEHGLFLNMVSKVVVGSEKGV
                     EILDAIRRK"
     gene            26135..26548
                     /gene="rpsL"
                     /locus_tag="ACG842_000432"
     CDS             26135..26548
                     /gene="rpsL"
                     /locus_tag="ACG842_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HFQ0246997.1"
                     /translation="MPTINQLVRKPRKSKVEKSNSPALNKGYNSFKKTQTNVNSPQKR
                     GVATRVGTMTPKKPNSALRKYARVRLSNLIEVTAYIPGIGHNLQEHSVVLLRGGRVKD
                     LPGVRYHIVRGALDTAGVNDRKQSRSKYGTKKPKA"
     gene            26632..27102
                     /gene="rpsG"
                     /locus_tag="ACG842_000433"
     CDS             26632..27102
                     /gene="rpsG"
                     /locus_tag="ACG842_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HFQ0246998.1"
                     /translation="MPRKGPVAKRDVLPDPIYNSKLVTRLINRVMVDGKRGIAANIIY
                     NAFDLIKESTGNDPLEVFEQAMKNVQPVLEVKARRVGGSNYQVPVEVRPERRVTLGLR
                     WVVNYARLRGEHTMEERLAKEIMDAANNTGASVKKREDTHKMADANRAFAHYRW"
     gene            27196..29283
                     /gene="fusA"
                     /locus_tag="ACG842_000434"
     CDS             27196..29283
                     /gene="fusA"
                     /locus_tag="ACG842_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HFQ0246999.1"
                     /translation="MTREFSLQNTRNIGIMAHIDAGKTTATERILYYTGKIHKIGETH
                     EGASQMDWMEQEQERGITITSAATTAQWKGHRINIIDTPGHVDFTVEVERSLRVLDGA
                     VTVLDAQSGVEPQTETVWRQATTYGVPRIVFANKMDKIGADFLYSVSTLHDRLQANAH
                     PIQLPIGAEDDFTGIIDLVTMKAEMYTNDLGTEIEETEIPEEYRELAEEWREKLVEAV
                     AETDEELTLKYLEGEEITEAELKEGIRRATVNVEFYPVLCGSAFKNKGVQLLLDAVLD
                     YLPSPLDIPAIKGIDPKTDEEVERPADDSAPFSALAFKVMTDPFVGRLTFFRVYSGVL
                     QSGSYVQNATKGKRERVGRILQMHANSRSEISEVYAGDIAAAVGLKDTTTGDTLCDEK
                     SLVILESMEFPEPVIQVAIEPKSKADQDKMGVALQKLSEEDPTFRAETNVETGETIIA
                     GMGELHLDIIVDRMRREFKVEANVGAPQVSYRETFRAGTQAEGKFVRQSGGKGQYGHV
                     WIEFTPNEEGAGFEFENAIVGGVVPREYIPAVETGLKDAMENGVLAGYPLVDIKAKLY
                     DGSYHDVDSNETAFRVAASMALRAAAKKANPVILEPIMAVEVVIPEDYLGDVMGHVTA
                     RRGRVEGMEARAGGQQVVRAMVPLAEMFGYATTLRSATQGRGTFTMTFDHYEDVPKSV
                     QEEIIKKNGGKAE"
     gene            29455..30642
                     /gene="tuf"
                     /locus_tag="ACG842_000435"
     CDS             29455..30642
                     /gene="tuf"
                     /locus_tag="ACG842_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HFQ0247000.1"
                     /translation="MAKEKFDRSKPHVNIGTIGHVDHGKTTLTAAITTVLSKKNGGQA
                     MAYDQIDGAPEERERGITISTAHVEYETDTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVSAADGPMPQTREHILLSRQVGVPYIVVFLNKVDMVDDEELLELVEMEVRDLLTE
                     YEFPGDDVPVVAGSALKALEGDASYEEKILELMAAVDEYIPTPERDNDKPFMMPVEDV
                     FSITGRGTVATGRVERGQVRVGDEVEVVGIAEETSKTTVTGVEMFRKLLDYAEAGDNI
                     GALLRGVAREDIQRGQVLAKPGTITPHTKFSAEVYVLTKEEGGRHTPFFTNYRPQFYF
                     RTTDVTGVVELPEGTEMVMPGDNVTMEVELIHPIAIENGTKFSIREGGRTVGAGVVTE
                     IKA"
     gene            31049..31357
                     /gene="rpsJ"
                     /locus_tag="ACG842_000436"
     CDS             31049..31357
                     /gene="rpsJ"
                     /locus_tag="ACG842_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HFQ0247001.1"
                     /translation="MAKQKIRIRLKAYEHRILDQSADKIVETAKRTGASVSGPIPLPT
                     ERSLYTIIRATHKYKDSREQFEMRTHKRLIDIVNPTPKTVDALMKLDLPSGVNIEIKL
                     "
     gene            31388..32017
                     /gene="rplC"
                     /locus_tag="ACG842_000437"
     CDS             31388..32017
                     /gene="rplC"
                     /locus_tag="ACG842_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HFQ0247002.1"
                     /translation="MTKGILGKKVGMTQIFTESGELIPVTVVEATPNVVLQVKTMETD
                     GYEAIQVGYQDMREVLSNKPAKGHVAKANTAPKRFIREFKNVELGEYEVGKEITVDVF
                     QAGDIIDVTGTTKGKGFQGVIKRHGQSRGPMAHGSRYHRRPGSMGPVAPNRVFKNKRL
                     AGRMGGNRVTIQNLEVVRVDAERNVILIKGNVPGAKKSLITIKSAVKAK"
     gene            32045..32668
                     /gene="rplD"
                     /locus_tag="ACG842_000438"
     CDS             32045..32668
                     /gene="rplD"
                     /locus_tag="ACG842_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HFQ0247003.1"
                     /translation="MPNVALFKQDGSQNGEITLNEEIFGIEPNESVVYDAIVMQRASL
                     RQGTHAVKNRSAVRGGGRKPWRQKGTGRARQGSIRSPQWRGGGVVFGPTPRSYSYKLP
                     KKVRRLAMKSVLSEKVAENNLVAIEGLNFDAPKTKEFKQVLANLSIDSKVLVVLEKGN
                     DFAALSARNLPNVSVVTSDNVSVLDVVSNTKVLATQTALTQIEEVLA"
     gene            32668..32958
                     /gene="rplW"
                     /locus_tag="ACG842_000439"
     CDS             32668..32958
                     /gene="rplW"
                     /locus_tag="ACG842_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HFQ0247004.1"
                     /translation="MNLLDVIKRPVITEKSMLAMDDKKYTFEVDTRANKTLVKQAVEA
                     AFDVKVKNVNIVNVRAKFKRMGKYAGYTKKRRKAIVTLTEDSKEIQLFEAAE"
     gene            32997..33830
                     /gene="rplB"
                     /locus_tag="ACG842_000440"
     CDS             32997..33830
                     /gene="rplB"
                     /locus_tag="ACG842_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010756442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HFQ0247005.1"
                     /translation="MAIKKYKPTSNGRRNMTGSDFAEITTSTPEKSLLQPLKNHAGRN
                     NNGRITVRHQGGGHKRQYRVIDFKRNKDNVVATVQTIEYDPNRSANIALVHYEDGVKA
                     YILAPKGLEVGMKLVSGPEADIKVGNALPLENIPVGTVIHNIEMKPGKGGQLIRSAGT
                     SAQVLGKEGKYVLIRLNSGEVRMILAACRATIGSVGNEQHELINIGKAGRSRWMRKRP
                     TVRGSVMNPNDHPHGGGEGKAPIGRKAPVSPWGQPALGYKTRNKKAKSDKLIVRRRKN
                     K"
     gene            33873..34151
                     /gene="rpsS"
                     /locus_tag="ACG842_000441"
     CDS             33873..34151
                     /gene="rpsS"
                     /locus_tag="ACG842_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HFQ0247006.1"
                     /translation="MGRSLKKGPFVDEHLMKKVEAQAGVEKKKVIKTWSRRSTVFPSF
                     VGYTIAVYDGRKHVPVYIQEDMVGHKLGEFAPTRTYRGHAADDKKTKR"
     gene            34174..34521
                     /gene="rplV"
                     /locus_tag="ACG842_000442"
     CDS             34174..34521
                     /gene="rplV"
                     /locus_tag="ACG842_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HFQ0247007.1"
                     /translation="MAEQITSAKATAKTVRTSPRKARLVIDLIRGKSVADAISILKFT
                     PNKSAGIIEKVLMSAVANAENNFDLDVENLVVSEAFVNEGPTMKRFRPRAKGSASPIN
                     KRTSHITVVVSEK"
     gene            34535..35191
                     /gene="rpsC"
                     /locus_tag="ACG842_000443"
     CDS             34535..35191
                     /gene="rpsC"
                     /locus_tag="ACG842_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HFQ0247008.1"
                     /translation="MGQKVHPIGMRVGIIRDWDAKWYAEKEYAEFLHEDLRIRKFIST
                     KLADAAVSTVEIERATGRVNISIHTAKPGMVIGKGGSEVESLRKELNKLTGKRIHINI
                     VEIKKPDLDAKLVGEGIARQLENRVAFRRAQKQAIQRSMRAGAQGIKTQVSGRLNGAD
                     IARSEGYSEGTVPLHTLRADIDYAWEEADTTYGKLGVKVWIYRGEILPTKKNTEKGGK
                     "
     gene            35194..35628
                     /gene="rplP"
                     /locus_tag="ACG842_000444"
     CDS             35194..35628
                     /gene="rplP"
                     /locus_tag="ACG842_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011375443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HFQ0247009.1"
                     /translation="MLVPKRVKHRREFRGKMRGEAKGGKEVAFGEYGLQAVDSHWITN
                     RQIEAARIAMTRYMKRGGKVWIKIFPHKSYTAKAIGVRMGSGKGAPEGWVAPVKRGKI
                     MFEIAGVPEEVAREALRLASHKLPMKTKIVKREEMGGESNEG"
     gene            35618..35806
                     /gene="rpmC"
                     /locus_tag="ACG842_000445"
     CDS             35618..35806
                     /gene="rpmC"
                     /locus_tag="ACG842_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HFQ0247010.1"
                     /translation="MKVKEIRELTTAEMLDQEKQLKEELFNLRFQLATGQLENTARIK
                     EVRKSIARIKTVLREQAK"
     gene            35831..36097
                     /gene="rpsQ"
                     /locus_tag="ACG842_000446"
     CDS             35831..36097
                     /gene="rpsQ"
                     /locus_tag="ACG842_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HFQ0247011.1"
                     /translation="MTEGRNQRKVYQGRVVSDKMDKTITVAVETKKNHPIYGKRMNYS
                     KKYKVHDENNTAKVGDIVRIMETRPLSATKRFRLLEVVEEAVII"
     gene            36155..36523
                     /gene="rplN"
                     /locus_tag="ACG842_000447"
     CDS             36155..36523
                     /gene="rplN"
                     /locus_tag="ACG842_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012727667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HFQ0247012.1"
                     /translation="MIQAESRLKVADNSGAREILTIKVLGGSGRKTANIGDVIVATVK
                     QATPGGVVKKGDVVKAVIVRTKSGARRTDGSYIKFDENAAVIIRDDKSPRGTRIFGPV
                     ARELRENNFMKIVSLAPEVL"
     gene            36560..36868
                     /gene="rplX"
                     /locus_tag="ACG842_000448"
     CDS             36560..36868
                     /gene="rplX"
                     /locus_tag="ACG842_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HFQ0247013.1"
                     /translation="MFVKKGDKVKVITGKDKNKEGVVLAAFPKKDKVIVEGVNVVKKH
                     QKPSQAAPQGGIVEMEAPIHVSNVMVVDSTGVAGRVGYKEVDGKKVRVSKKTGEVLDK
                     "
     gene            36896..37435
                     /gene="rplE"
                     /locus_tag="ACG842_000449"
     CDS             36896..37435
                     /gene="rplE"
                     /locus_tag="ACG842_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016185989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HFQ0247014.1"
                     /translation="MNRLKEKYLKEVTPSLMEKFNYSSVMQTPKVEKIVINMGVGDAV
                     SNAKNLDKAVEELTLITGQKPMITKAKKSIAGFRLREGMPIGAKVTLRGERMYEFLDK
                     LVSVSLPRVRDFHGVSKKAFDGRGNYTLGIKEQLIFPEVDYDLVDKVRGMDIVIVTTA
                     NSDEESRELLTQLGMPFQK"
     gene            37454..37639
                     /locus_tag="ACG842_000450"
     CDS             37454..37639
                     /locus_tag="ACG842_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011245027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HFQ0247015.1"
                     /translation="MAKKSMIAKNKRPAKFSTQAYTRCERCGRPHSVYRKFHLCRICF
                     RELAYKGQIPGVKKASW"
     gene            37675..38073
                     /gene="rpsH"
                     /locus_tag="ACG842_000451"
     CDS             37675..38073
                     /gene="rpsH"
                     /locus_tag="ACG842_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010742763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HFQ0247016.1"
                     /translation="MVMTDPIADFLTRIRNANMVKHESLEVPASKIKRDIAEILKREG
                     FVRDVEYIEDDKQGVIRVFLKFGKNGERVITNLKRISKPGLRAYVKADEVPKVLNGLG
                     IAIISTSEGVVTDKEAREKNIGGEVIAYVW"
     gene            38105..38641
                     /gene="rplF"
                     /locus_tag="ACG842_000452"
     CDS             38105..38641
                     /gene="rplF"
                     /locus_tag="ACG842_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HFQ0247017.1"
                     /translation="MSRIGNKVVVIPEGVTVTQDGNNVTVKGPKGELTRTFSADIKMN
                     IEGNEVTFTRPNDSKEMKTIHGTTRANFNNMVVGVSEGFQKALELIGVGYRAQLQGSK
                     LVLNVGYSHPVEITPPAGVTVEVPANTQVIVKGANKEEVGELAANIRGTRPPEPYKGK
                     GIRYVGEYVRRKEGKTGK"
     gene            38802..39158
                     /gene="rplR"
                     /locus_tag="ACG842_000453"
     CDS             38802..39158
                     /gene="rplR"
                     /locus_tag="ACG842_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014125626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HFQ0247018.1"
                     /translation="MITKPDKNKTRQKRHRRVRNNISGTAERPRLNVFRSNKNIYAQI
                     IDDVAGVTLASASTLDKEISGGTKTEAAQAVGKLVAERAAEKGIKVVVFDRGGYLYHG
                     RVQALAEAARENGLEF"
     gene            39179..39679
                     /gene="rpsE"
                     /locus_tag="ACG842_000454"
     CDS             39179..39679
                     /gene="rpsE"
                     /locus_tag="ACG842_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HFQ0247019.1"
                     /translation="MVYIDPKHLELEDRVVAINRVTKVVKGGRRLRFAALVVVGDKNG
                     HVGFGTGKAQEVPEAIRKAIEDAKKNLVEVPMVGSTIPHEVIGAFSGGRILMKPAVEG
                     SGVAAGGPVRAVLELAGVADITSKSLGSNTPINVVRATVEGLKQLKRAEEVAELRGKS
                     VEELIG"
     gene            39694..39873
                     /gene="rpmD"
                     /locus_tag="ACG842_000455"
     CDS             39694..39873
                     /gene="rpmD"
                     /locus_tag="ACG842_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HFQ0247020.1"
                     /translation="MAELKVTLKRSIIGRPQNQRDTVKALGLTKINSSVVKPSNEAIK
                     GMINTVSHLVDVEEI"
     gene            39917..40357
                     /gene="rplO"
                     /locus_tag="ACG842_000456"
     CDS             39917..40357
                     /gene="rplO"
                     /locus_tag="ACG842_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005592004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HFQ0247021.1"
                     /translation="MKLHELKPAEGSRQVRNRVGRGTSSGNGKTAGRGQKGQKARSGG
                     GVRLGFEGGQTPLFRRLPKRGFTNVNRKDYAVINLDVLNRFEDGAEVTPVALVEAGIV
                     KNEKAGIKVLANGELNKKLTVKAAKFSKAAQEAIEAAGGSIEVI"
     gene            40357..41652
                     /gene="secY"
                     /locus_tag="ACG842_000457"
     CDS             40357..41652
                     /gene="secY"
                     /locus_tag="ACG842_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HFQ0247022.1"
                     /translation="MFRLLKDAFKVKDIRSKILFTVFVLFVFRLGAHITVPGVNAASL
                     QNLSSLPFFNMLNLVSGSAMQNFSIFSMGVSPYITASIVIQLLQMDIVPRFVEWSKQG
                     EVGRKKLNQATRYLTLVLAFIQSVGITAGFNQYAQLGFVNNPNVATYVTIGLILTAGT
                     MFVTWLGEQITEKGIGNGVSMIIFAGIISRLPESIREIYEDYFINIDPSDIWKSAIFV
                     AILLVAVLLIVTFVTFFQQAERKIPIQYTKRVAGAPTSSYLPLKVNAAGVIPVIFASS
                     FIATPNAVLQALSGRFGSENWYKVMMEIFNYNTAPGATIYTILIVAFTFFYAFVQVNP
                     EKLAENLQKQGSYIPSVRPGKGTEEYVSRLLMRLSTVGAVFLGLVALLPIIAQMVWDL
                     PQSIGLGGTSLLIVIGVALETAKQLEGLMLKRKYVGFIE"
     gene            41714..42361
                     /locus_tag="ACG842_000458"
     CDS             41714..42361
                     /locus_tag="ACG842_000458"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HFQ0247023.1"
                     /translation="MNLILMGLPGAGKGTQAEKIIDAYGIPHISTGDMFRAAMQNETA
                     LGLEAKSYMDKGALVPDEVTNGIVKERLAEPDTEKGFLLDGFPRTLDQAKALDAMLKD
                     LNKKIDAVIDIHVGEEILVERLAGRFICSNCGATYHKIFNPTKVEDTCDRCGGHEFYQ
                     REDDKPETVKNRLAINIKNSEPILAYYKEQGLLNTIDGAREIDAVFADVQKIIEK"
     gene            42552..42770
                     /gene="infA"
                     /locus_tag="ACG842_000459"
     CDS             42552..42770
                     /gene="infA"
                     /locus_tag="ACG842_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HFQ0247024.1"
                     /translation="MAKEDMIEVEGTVVETLPNAMFKVELENGHQVLATVSGKIRMHY
                     IRILPGDKVTVELSPYDLNRGRITYRFK"
     gene            42803..42919
                     /gene="rpmJ"
                     /locus_tag="ACG842_000460"
     CDS             42803..42919
                     /gene="rpmJ"
                     /locus_tag="ACG842_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HFQ0247025.1"
                     /translation="MKVRPSVKPMCEHCKVIRRKGRVMVICPANPKHKQRQG"
     gene            42937..43302
                     /gene="rpsM"
                     /locus_tag="ACG842_000461"
     CDS             42937..43302
                     /gene="rpsM"
                     /locus_tag="ACG842_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HFQ0247026.1"
                     /translation="MARIAGVDIPRDKRVVVSLTYIYGIGNTTAKKILADAGVSEDVR
                     VRELTNEQTDAIRAEVDKLKVEGDLRREVNLNIKRLMEIGSYRGIRHRRGLPVRGQNT
                     KNNARTRKGPARAIAGKKK"
     gene            43330..43719
                     /gene="rpsK"
                     /locus_tag="ACG842_000462"
     CDS             43330..43719
                     /gene="rpsK"
                     /locus_tag="ACG842_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HFQ0247027.1"
                     /translation="MAAKKVNRKRRVKKNIEAGIAHIHSTFNNTIIMITDTHGNALAW
                     SSAGSLGFKGSKKSTPFAAQMAAETATKAAMEHGLKTVEVTVKGPGSGREAAIRSLQA
                     TGLEVTAIRDVTPVPHNGARPPKRRRV"
     gene            43799..44737
                     /locus_tag="ACG842_000463"
     CDS             43799..44737
                     /locus_tag="ACG842_000463"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010774253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HFQ0247028.1"
                     /translation="MIEFEKPRIAKIDEEKDYGKFIVEPLERGYGTTLGNSLRRILLS
                     SLPGAAITNIQIDGVLHEFSTIKGVREDVTQIILNIKGLALKMYGQEEKTLEIDITGP
                     ATVTAGDIIVDSDVEILNKDMYICSVSEGATFHARLTVKPGRGYVQADENKKEDMPIG
                     VLPVDSIYTPVRRVNYQVENTRVGHREDFDKLTMEIWTDGSIEPLEAMSLSAKIMTEH
                     LDIFVNLTDEAKNAEIMVEKEETQKEKMLEMTIEELDLSVRSYNCLKRAGINTVQELT
                     NKSEPEMIKVRNLGRKSLEEVKAKLHDLGLGLRKED"
     gene            44765..45145
                     /gene="rplQ"
                     /locus_tag="ACG842_000464"
     CDS             44765..45145
                     /gene="rplQ"
                     /locus_tag="ACG842_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HFQ0247029.1"
                     /translation="MSYRKLGRTSSQRKAMLRDLTTDLLINERIVTTEARAKEVRSTA
                     EKMITLGKRGDLHARRQAAAFVRNEVASVREENEDIVIESALQKLFNDIAPRYAERQG
                     GYTRILKTEPRRGDGAPMVVLELV"
     gene            45417..46256
                     /locus_tag="ACG842_000465"
     CDS             45417..46256
                     /locus_tag="ACG842_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HFQ0247030.1"
                     /translation="MEPIIELEKINYKYQPDDLRPALKDVSFTIDKGEWIAIIGHNGS
                     GKSTLAKTINGLLLPESGIVKVGNQILDEENIWTIRQMVGMVFQNPDNQFVGSTVEDD
                     VAFGLENQGIPREEMLVRVKDALEKVRMAEFASREPARLSGGQKQRVAIAGVVALRPD
                     IIILDEATSMLDPEGREEVISTIKKIKEESQLTVISITHDIDEAANANRILVMRQGEL
                     VREGTPKEIFSAGPELIDLGLDLPFPEKLKSALKERGVDVPSEYMTEERMVDWLWTSV
                     LNK"
     gene            46232..47101
                     /locus_tag="ACG842_000466"
     CDS             46232..47101
                     /locus_tag="ACG842_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HFQ0247031.1"
                     /translation="MDIRFEQVDFTYQPNTPFEQRALFDINMTIKENSYTALVGHTGS
                     GKSTLLQHLNALVKPTSGTVHIGERDIQPDTDNKNLKPIRKKVGIVFQFPEAQLFEET
                     VAKDIAFGPKNFGVSEEEALVLAKETLEQVGLDESYLERSPFELSGGQMRRVAIAGVL
                     AMRPEVLVLDEPTAGLDPQGRKEMMEMFWRLHKEQQITIVLVTHLMDDVANFADYVYV
                     LEKGRIVNSGEPQEVFQNIEWLKEKQLGVPTATEFAEELMAKGMNFATLPLTAEELAD
                     AIVHYAGGNAHDE"
     gene            47091..47888
                     /locus_tag="ACG842_000467"
     CDS             47091..47888
                     /locus_tag="ACG842_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HFQ0247032.1"
                     /translation="MMNKLIFGRYMPGDSLIHRLDPRAKLLASFYFIGIIFLANNWET
                     YLLLAFFTLLSVFLSKVDLKFFIRGIRPLIWLILFTVALQVLFTSGGETYWSWGVFHI
                     TEFGVMNGLFIFCRFVLIIFMSTLLTLTTPPLELSDAIEYLLRPLKAVRFPVHEISLM
                     LSIALRFVPTLMDETEKIMNAQRARGVDFGEGNLVQKMKAVIPLLIPLFVSSFNRAED
                     LATAMEARGYKGGEGRTKYRVLHWHNRDTMVIIAFVLLTVLLIFLRG"
BASE COUNT        15190 a         8780 c        10522 g        13541 t
ORIGIN      
        1 agctacacac ttctatataa attcagaagt tcttctagag tttctaaagt ttgtttctta
       61 attagcttat tgattctata atatttttga tttatatagg aaattaaaaa tattatagaa
      121 tcaacactca gagaacgggt tggtttttaa tagatttaat aagtactgat aatgaaattt
      181 ctcctggttg ggaaaacgaa gttggttacc ctgtagaaga attttttcaa gattactttg
      241 cggagtatgc cgataggata atgagagatt acgtaacaga taaaactcaa gaagtcatca
      301 atcaatccca gtcattaaat gaattatctg ataaggcgaa agaagaagca gttgccatta
      361 tttcttcagg gattagtgac tatattgata tgagtggtga aatcaattat ggcgataaag
      421 acaaaattgt acgcatgata aaagctttta tcaaaacttt tgatgtgaag gcagatctta
      481 agtttaatga tacttaccta gtaggaaaat taattaatat ccttgggaca gagaaagata
      541 cggaaacgtt tatttcctct ttgtctatgt tgtttacaga taagcatttt tcaactcagc
      601 ttggtggaga ggaactagtg gaaatctttt aaccattacg cccagcatta ataaaagtat
      661 atcaaaaaag tactcctgat gatttttaag attggtttga gaaataatgt gaaaacacta
      721 tttttaatgt taattataga tagagatgaa cgcgtcatat ggttaaaaga atgaaaaagt
      781 caaagataca ctgctatatt tcactaagga tatagcagtg tatctttcat gtcgtttcac
      841 tggcagtttt ttataaaagg aggtgtgcat ttcagttgct tttctttggt agttagttga
      901 atcgtttaag aattaacgga tgtggcagac aggtgctctg tccaaaaacc agtaaaatct
      961 ttgatggttc catctttatt tagctgtgcc tgttcaaata ctgttttgtt tttaccgaat
     1021 ggatcaattg caataattgt tccgatcccc gcacgtttgg cagcaagtaa tcctgagtaa
     1081 gcatcttcga taacaagaca atcagccgga gctaagctca gtttgtctgc cgctttaaga
     1141 aaaatatccg gttgcggttt cccagggaaa gagccgtcgt catagacaat cttgtcccag
     1201 tcaaaccagc gggctaaatc gaaaatatca aaataaaaag caacattctc tttgacagta
     1261 gccgtggcaa tcgttaatgg aatctgttgg tcagtcagct ggtcaagagt cgcttcaagc
     1321 ccttttgtca actgaagctc gttttcattt tgcaagcaaa gcatacgata atagctttct
     1381 ttttcttctg acaatacggc gatctcagag gcggtcaaat gctctgaaat gaaatgtttc
     1441 aaaatctccg tattcgtccg accatgaata ttttttaaaa tatcctcgtc tgataaacca
     1501 tcttttgtat gatcatgtat catatgcagc caagcagctt catgcagatg agagtccaaa
     1561 aacattgttc cgttgaaatc aaatataatc cctttcatag ttacgctcct atagtggtat
     1621 tttcggttga atcagcaatt tgcagatcga catattgtgc atagtgctct tgaacctccc
     1681 gcgagatctg gcgatccgta atgagcagat cgaaatcatg caaattataa aacacataaa
     1741 aatcatctcg attgaatttt ttattatcaa cgagtagata tttcttacgg gaattattta
     1801 gagcgatctg ctgtgcttct ccttcttcgg tactgtaagt agagatcgct tcgttgtgaa
     1861 cgccatttga actgataaat gcttttgtaa actttagtct tcttaaattg tcgttggtga
     1921 tcggtccgac aaatgcgccg gtatttttcc ggaaatcacc gccaatcagt agaatttctg
     1981 gtccatctgc ttgagcaaga atatcaaaaa ctggataatt atttgtaatg atccgaattt
     2041 tcttgttcag taaatgcgtc gccaatagtt cgattgttgt acctggtcct aagaaaatcg
     2101 tttctccctc gtgaatcaag gaggctgctg tggcagcaat ttcttttttt tcttgaatct
     2161 gcagagtggc tttttcagtg tgagaaagtt cttgatctaa agtgtaggaa agactttgag
     2221 cccctccatg gacacgcagc agcttgcccg atttttctaa ttcatctaaa tctctgcgaa
     2281 cggtcatatc agaaacatct aattgattga tgatttcatt aacggtaagg attccttttt
     2341 tattgaccat ttctacgata gtaagcaatc tttctttttt caacattgtc ttcacctcta
     2401 atttaaggat aatctatcat ataaacggac aacagtcaat ctaattcgaa caaaagtaaa
     2461 catttagggt ggtttttatg tttgttacaa tagattttat tgctgttttc tggtttttat
     2521 gattatttgt gtttgttttt gttgacaaat gttttggatg cgtttacaat gcaatcagtt
     2581 agaaagagcg aaaactgatt atagatttga ggagagatgt acatgcttaa attaccagaa
     2641 gattttattt ttggcggagc cacagcagcc tatcaggttg aaggagccac taaggaaggt
     2701 ggcaaaggcg ctgttgcttg ggatgacttt ttagaggaac aagggcgatt cagtcccgat
     2761 ccggcaagtg atttttatca tcaatatgca aaggacattg aattgtgtga acgttttggt
     2821 gtaaatggtc taagattatc cattgcttgg agccgcattt ttcctgatgg agctgggaaa
     2881 ccaaatccag aaggaatcgc tttttatcat cgtgtgttcg aagaatgtaa gaaaagaaac
     2941 gtaacgccgt ttgtgacgtt gcatcatttt gataccccta aacgtttgtt tgaccatgga
     3001 gattttttga atcgagaaac aatagaagca tttgtttcct atgccatttt ttgttttcat
     3061 gaattcaaag aagtcaaggt gtggagtacc tttaatgaaa tctatccagt tgcaaccaat
     3121 caatatttgt taggtgtttt cccaccagga atcaaatatg attttacaaa gatcgtcgcc
     3181 tgtcttcata atatgatggt tgctcatgcc cgtgtagtga actatttcaa agaaaacgaa
     3241 cttccgggtg aaattggtgt agttcactca ttagagacga aatatgcggc aacagacgcg
     3301 ccggaagata agcatgctgc ctttttagac gatgcattgt cgatccgttt cttattagat
     3361 gctacctatt taggctatta ttcaactgaa accttgactg ctcttgatga gatatgtgaa
     3421 gcaaatcagg ccagctatca ttttcctgaa gaagatttcg ttgaattgaa aaaagcatcc
     3481 acgcgtaatg actacttagg gatcaatcac taccaatgcc attttgtcaa agcttacgat
     3541 ggagaaaatg caatccatca taacggtacg ggtgaaaaag ggacctctgt ttataaagtg
     3601 aagggaatcg gagaacgtat ttataaagaa ggaattcctc gaactgattg ggactggctg
     3661 atttatccag aaggtctata tgacttgttg ctgcgaatta aaagcgatta cccacattac
     3721 aacaaaatct atatcactga gaacgggatg gggtataaag atcagtttga agatggcatt
     3781 attatggatc agccgagaat cgactatttg agagtctatt tggaatcctt aagcaaggcg
     3841 ataacagccg gggtaaatgt gaaggggtat tttttgtggt cgcttatgga tcttttctca
     3901 tggacaaatg ggtataacaa aaggtatggc ttgttttatg ttgattttga aacccagaag
     3961 cgttacccaa aagaatcagc ttactggtac aagttggtca gtgaaaccaa aacgattatt
     4021 taataagtga taatggagga agaaaagatg aataaagagg aattacaaat gcttggtttt
     4081 gagatcgttg catattcagg cgacgctcgc agtacattat tgaaattatt gaaagaagta
     4141 agacaaggaa actttgaaca tgttgattcg gcgttgaaag aagccgacga aaatttaacc
     4201 cttgcacata attcgcaaac aaaaatttta gcggaagaag catctgggaa agagatggag
     4261 atgggattca tttttatcca tgggcaggat catttaatga ccactttatt gctgagagat
     4321 ctgattcaag actttattgt actgtacaga cagaatcaag gataggagaa gaacgctatg
     4381 gatggaatca ttaaacaaat cgaaaaaggg aaaccatttt ttgaaaaaat ctcacgtaat
     4441 agttatttag gagccgttcg tgacggattt ttaacagcga tgccagctat tttattttca
     4501 agcattttca ttttgattgc tgcggttcct gagatttttg gttaccaatg gcctgaatct
     4561 gtctccacat ggctatggaa agtctataat tattcgatgg gtgttgtagg gttactcgtt
     4621 tcagcaacca ctgctcgctg cttggctgat tcgatgaatc gtcgcttacc gaaaaacaaa
     4681 gtaatcaaca ccacctctgt gatgctggcc tctgtggtaa gtttcctgtt gttaagcgtc
     4741 tctccgattg aaggtggatt ttcaacggaa tatatgggga caaagggact actggcttcc
     4801 tttgtcgctg catttttaac agttaattta tataagtttt gtgtgctgaa agatattacc
     4861 attaaaatgc ctaaagaagt tcctggaacg atttcgcaaa ccttccgaga tatttttcct
     4921 ttctcttttt cagtatttgc ggccgtttta attgatttag gcattcgaca aatcttccaa
     4981 atctcatttg cagaagcaat cattacggtg ctgcagccgc tatttacagc agctgacggg
     5041 tacttaggaa tcgctattat ctggggagcg atggcgctgt tttggtttgt cggtgttcat
     5101 ggaccgtcga tcgtggagcc ggcaattgcg gcaattatct atgccaatgt ggaaaccaat
     5161 ctgcaattgt tcaattccgg tgaacaagct tctaaagtac tgacggtggg tttagggaat
     5221 tttgtaggaa ctatgggagg aactggggca acattggttg ttccttttat cttcctactc
     5281 tttgctaagt ctaagcagtt gaaggcagtt gggaaagctt cctttattcc agtaagtttc
     5341 gctgtcaacg aaccattgct atttgcggca cccatgattt tgaaccctta tttttttatt
     5401 ccatttttgt taacacctgt tgtaaacgtt tgcctgttca agttttttgt cgatgtattg
     5461 ggcatgaaca gctttatgta cgttttgcct tgggcaactc cagcaccgat cggtttggtt
     5521 ctaggaacgg gcatgggtat cttagctttt gttctagcag cggttctaat cgtcgttgac
     5581 ttcctgatct atctaccatt ttgcaaagca tacgatgctg tcttagtaga gaaagaagca
     5641 caaaaagcag aggtgatcgc aaccgaggaa ggtgcttctg ccgatgtacg tgaggaagta
     5701 cctgtggcag cgagtcaagt tatcagcgat gcaaataaag aaatcaatgt gttggtactt
     5761 tgtgctggcg cagggacaag tgggatgtta gctaatgctt taaaaaaagg tgcatctgaa
     5821 tcgaaagcac cgatcagtgc aatggcgggt gcttatggtt ctcattacga aatgctgcag
     5881 gattttgata tggtggtctt agcaccgcaa gtcaattctt actatgatga tatcaaagaa
     5941 gacaccgatc gcttaggaat caagctggtt gcgacaaaag gagcagaata tatcggatta
     6001 acgagagatc ctgaaaaagc tgttcagttt attctgaacc aatttcaata aaggaggagc
     6061 cataatgaaa atgggaattt ggttgcgtaa agctgtttta tttgcagctg tgttaggggc
     6121 tttgggggct gtttcaaaaa ctgttgaagc atctgccagc gaatttattc gcggagcaga
     6181 tatttcgatc ctgtcagata tggaaaaatc tggtgcaaaa tattatgaag acgatatttc
     6241 aaaagatgcc ttagagattt taaaaaataa tggtgtcaac tatgttcgac tgcgtttgtg
     6301 gcaggatcct tataatagta atggagaaag ttatggagca gggacaaatg atctggaaac
     6361 gacgattgac cttgcaaaac gagcaaaaaa tctgggactt aaggtgctgc tggattttca
     6421 ctacagtgat ttttgggttg atcctgggaa acagaatctt cctaaagcat ggcaaggtct
     6481 gacctttgaa gaaatgaata cggcattgta cgactataca aagaacgttc tgtcggaaat
     6541 gaagcagctg gacgtttatc ctgatatggt tcagatcggc aatgaactga atagcggaat
     6601 gctctggcct tatggcaagt cttggggaga aggcggggga gaatttgatc gattggctgc
     6661 ttttttgaag tctggtatcc aagctgttcg agacactcaa cctaaaacaa ctccagtaat
     6721 gctccactta gctgatggtg gcgatacagg agcatttact tggtggtttg atgaaatcac
     6781 gagtcgaggt gtgtcatttg atttaattgg agtgtcgtat tatccgtatt ggcacggttc
     6841 aatgaatgaa cttcaaacca atatgaatgc gatcagccaa cggtatggta aagaagtgat
     6901 cgtagttgaa acagcttatg ggcatacaac agcgaatgcc gatactatgc caaatgcttt
     6961 tggtgaagcc gaagcagctg cggggggata tgaaccaaca ccgacaggac aagcagagta
     7021 tttgctggat cttgccgatc ggattcaggc agtaccgaat aatcgcgggg cgggcttctt
     7081 ctattgggaa cctctttggt acaacggaaa tgtgagttgg gcaacacaag caggtatgaa
     7141 ttatctcagt gttcaatcca ctatgggaaa cgaatgggac aaccaagcga tgtttgattt
     7201 tgctggaaac gccttgccaa gcttgcgtgc gtttaaacaa gcaggggcgc aaataaatct
     7261 agttaaaaat gcaagtttcg aaagcgatgg accaacgacc agtccttcca attggcaaac
     7321 ctggtttcaa caaggaaccg ctaagggtac gatcaaaacg gaagcgggca ctttttttgg
     7381 ggactacaaa ctaacttttt ggcaggatca agcatacgag gcatcagctt atcaaaatgt
     7441 tagtggcata agcaaaggaa cttacacatt gagtgcctat gtaatgtctg ggggagagtt
     7501 tgatactaat cagttatata tcaaaaatta tggtggagcg gaactaaata ctgcggttgt
     7561 taaaacaagt gaatggacaa aaattacaat caaagacatt ccaattacaa caggtgtttg
     7621 tgaaattggg gtttatagca aggcttcagg aaatgcttgg acgaacgttg attttgtttc
     7681 attggtgaaa caatagactg cggatcgttt cgattcaaga tcggaggttc ccttcagcca
     7741 catcgttagc gtagaaggca ggaatcgaac ctgcacctcg cctttgccaa aaataaccag
     7801 aaacgatttc cagtcatttt taccaaaacg agtaaagcac cagacttcat ctacactttg
     7861 tattatgcta aagagagttc aacgatttcg taagaagcaa tgaaaaaatt gcaaaaagtt
     7921 gtgcagataa cataaagttt gtttaagaaa gggtaacctt agaattccgt cgtgaatatg
     7981 atttgagcac ttagatggat agaaaaaagt tctcggaaaa cagttcgttt ttcgagaact
     8041 tctttttatg ataagaatga aacaaattaa cttatattat agtctctttt ggtagggaac
     8101 catcttattt ttaatgaaaa tcaagcattt tagctattga agaatgttga tttataggga
     8161 ttccccctcc atattatgtt ggctctttat tcccactcca ctgttgctgg tggtttagaa
     8221 gtgatgtcat acacgatacg gttgacgtgg gctacttcgt ttacgatacg tacagagatt
     8281 ttttgcaaga cttcccatgg gatacgggca aagtcagcgg tcattccgtc gattgaagtt
     8341 acagcacgga taccgactgt gtagtcgtaa gtacgtccgt cacccataac accgacagag
     8401 cggatccctg gaagaacagt gaaatattgc cagatatcac ggtcaagtcc agcagctgcg
     8461 atttcttcac gcaagatagc atcagattcg cgtacgattt ctagcttgtc ttcagtgatt
     8521 tcgccaagaa cacgaatacc aagaccagga ccagggaatg gctgacgcca aacgattgaa
     8581 tcaggcatgc ctaactgagt ccctaattca cggacttcgt ctttgaataa tgtgtttaat
     8641 ggttcgatca attcaaattg catatcttct ggcagtccac ctacattatg gtgagatttg
     8701 atcgtctgtg ctgtttctgt accagattcg ataacgtcag tatacaacgt tccttgtgct
     8761 aggaaagaga cgccttcttc accagcaagt tttgttgctt cgtcatcaaa aacatatacg
     8821 aattcattcc caatgatttt acgtttttgt tctgggtcag atacaccttt caacttgtct
     8881 aagaagcgat ctcttgcatt gactttgatg atgttcaagc cgaattttcc gcctagactt
     8941 tccattactt gttccgcttc gcctttgcgt agcagaccgt ggtcaacaaa gatagatgtc
     9001 aattggtcac cgatcgcttt ttgtaaaaga accccaacga cactagaatc aacgccacct
     9061 gaaagaccaa gcaagacttt tttgtcacca acttgttcac ggattttttc tacttgcata
     9121 tcgataaagt tatccattgt ccagttacct tcacagtgac aaacatcaaa ggcaaagtgg
     9181 cgcagcaatt cgattccgta ttcggaatga cgtacttctg catggaattg gataccataa
     9241 aaattacgtg ttgtgtcttg gatagaagca atcggacaat ctttgcttgt tccgactgtt
     9301 tcaaagcctt caggaacttt agttaccaag tcaccatggc tcatccatac tgtttgttgt
     9361 tttggtgttt ctttaaacaa gacagcttga tcgcttgtta cttctaacaa ggctttccca
     9421 tattcgcggt ttttagcagg ctctacggaa ccgccaagtt tgtacgtgat caattgcata
     9481 ccatagcaaa tccctaatac aggaatacct aaattgaaga tctcaggatc gatcccaaat
     9541 gcattctcat catatacact gtttggtccg ccagaaagaa tgattccctt tggattcatc
     9601 gcttttactt cttctgcagt aatgcggtgg ctcaataatt cggaaaagac tccaagatca
     9661 cgaatacgtc ttgtgatcaa ttggttatac tgactaccgt agtctaatac gataatcttt
     9721 tcaacgttcg tcatatcggc aacgtttgtc aaatttatta cccctatccc tttaaatttt
     9781 atctcaaaat agtcaaacta ttttaaaatg ctaatatttt cgcatgaaaa tcgaatcgat
     9841 gatatgattt tcagttttat ggagaattat atcggctctt cctcgtgtag gcaagatata
     9901 ttcctgtaaa tttttcaaat tcacattttt ccatacatct tttgccattt taaaggcttc
     9961 ttcacgatct ccgatagcaa aacgataata ataattctct ggattttgga aagccgtatc
    10021 cagtaacgca ccaaaacgat ccaaatacca tttttcgatc agcacgggat cggcatcgac
    10081 atagacagaa aaatcaaaaa agtcgctgac gtagatttgc tgattcgcag gtaattgaag
    10141 cgtattgatc ccctcaacga tcaagatatc aggctgctgg atcaattcgt acttgtctgg
    10201 aatgatatca tacacatcat gtgagtaaac aggtactttt atctcttcct gaccagattt
    10261 tacacggttc aagaaatcga tcagcatctc catatcataa ctttcaggaa atcctttacg
    10321 gtccatgatt ccacgctctt ttaatatacg atttggataa agaaatccgt cagtcgtgat
    10381 cagctgaacg tttcttcgtt tgaaggtacg agacaaaatc gtctgcagca gtctggctgt
    10441 tgtacttttt ccaacggcta cacttccggc gatcccgata ataaaaggcg gtacttctac
    10501 gtatttatga agaaataaac ctttactcaa gaacaaagat tcaaattctt tcatataaag
    10561 ataaatcaga tgagcaagag gtacatagat atctcgaaca tcctgcatgg aaatgcggtc
    10621 attcaaactt ttgatattat ctagttcatc ttgtgtcaaa ggtgcttgcc cattacgata
    10681 aaattctcgc cattcttctc ttgccacacg gtagtagttc attggatcat tcatattagc
    10741 ctcctgcgtt ctcccatcaa tggaactatt ttaacacaaa ataaacgcaa accccaccac
    10801 ttaagagaga ttgccgatct ttttgtataa tctttataat agaaagaaac ggagtcttat
    10861 tcttgacttg cgcacggata cataaaagga tagactgttg tcatgaaaga aatgagaagg
    10921 atggaagaca tgacaaatca ttactattct gaacgcccag atacggcgca cgattttgaa
    10981 caatgggctt ttgagttaaa aggcagaacc tttcaatttg taacagattc tggtgtattt
    11041 tcacgtgaaa cagtggatta tggttcacgt gtcctgattg atgctttcga ttgggaagag
    11101 ttacctgatg aaggaaagat cttagatgtg ggatgcgggt atggaccgat tgggttagct
    11161 ttggcatttg cgacacagcg gtttgttgaa atggtcgaca ttaatgaacg agcggtaagt
    11221 ctagcgcaag gaaacgcaaa gcgtaaccaa atcgaacatg ttgatatcca tcaatcgaat
    11281 atctatgaag cagttcatga agaaacctat gcagctatcg taagcaatcc gccgatccgt
    11341 gctggaaaaa aagttgtaca tgaaattttg acaggcgcat atcctcggtt gaagaagggc
    11401 ggtacactga caattgtgat ccagaagaaa caaggagcgc cgagtgcgca aaagaaaatg
    11461 gaagaaacat ttggtaatgc agaaatcgtc acaaaagata aaggatatta tatcatcaaa
    11521 agtgtgaaag aataactagc tgaaaaatag tggagcgacg aaagagtgga actgggcgtg
    11581 catgtacggg tacgtatcag ttcttttttt gttgagaagt ttttaaaaac aaagaactat
    11641 tttttcttca aaaagaaata gtaggaacag agtcgttgtt ttttacgata aaataaataa
    11701 gatacagaac ttgtatagaa ggctgccaag tcggtagata aaggaggttt ttatcaaaaa
    11761 acatactttc cttttttctc tattcttttc ttgggggttt tggtacactg atagggaatt
    11821 tagatagaag ggagccaata ggatgaagct gaccatcaga gatatcgcag aaatggcagg
    11881 cgtatcagta acaactgtct cacagatttt gaataacaaa ggaagcagat ttagtgataa
    11941 gacaagaaaa aaagtgttgt cgatcgtgaa tgaacatcat tataagcccg attatttcgc
    12001 ttcaaatgtg atcaatcgac attcaaagac catcggaatg atcgtccctg atgtcactga
    12061 tttcttcttc tccaaactga tcgaaggtgt agagagctat ttgaatcctt tgggctatgt
    12121 gatcatgtta tgcaattctc gtcatagcca agaaaatgag atcaagtatt tacaagagct
    12181 ttcccaccgc tcagtggacg gaatcttgct ggccacacca aatattttac ctgagaaata
    12241 tgcattagac agcggttttt atcaaaaaat gccaatcatt ttaatcgatc gcggattgaa
    12301 tcgtcgggat aacggacggc ttatcgtaaa ggaatatgag ggcgcctatc aagctgtttc
    12361 gctgtttatt gaaaatggcc atacgaagat cggcatgctg aaggaaacca ctggatacta
    12421 tcaattagaa gaacgtttca acggatatcg tcatgcgctg aaagatcatg gcctgccttt
    12481 taatgggaaa tttgttgaac atggagagtt gactgtccaa ggtggatata ctgcatcaaa
    12541 aaaattactc aaacataaag atatcacggc tattttttgc gggaatgatg ccatggcgat
    12601 cggatgctat caggcgatcg atgaattggg caaaaaaatc ccagaagata tctcagtcat
    12661 aggattcgat ggattaaagc tgtcagaata catgattccg caattgacga cagtgcagca
    12721 gccaagtttt gatattggat tttatgctgc acgtttcctg atcgatacga tcgaatttcc
    12781 tcaaagaaaa gttccgaaca aagtgttcga aacaaaatta attattcgtg agagcgtaaa
    12841 agtattgaca aagggctaac tcggcggtat attgcttatg taagcattta caaaggctag
    12901 ttagtgcttt ttttattttt agtaaaccag tttactaaag tggtttacct ttcctaaaaa
    12961 taatgtataa taccgaatga agacaaggag gatcaggcaa tgcgaatggt ggatctgatt
    13021 gaaaaaaaga gggatggaaa cgagctgtcg aaagaggaaa tcgagtacat cgtcacgaac
    13081 tatacgaatg gaaagattcc agattatcaa gtcagtgcat tattgatggc aattttttat
    13141 caagacatga caaatgaaga aatcacgaac ttaacattgg cgattgctaa ttctggtgat
    13201 gtcatcgatt tgagttctct tgaaggaata aaggtagata agcattcgac aggtggcgtt
    13261 ggtgacacga ccacattgat tttagccccc ctagtagcta gtgtcggagt gacagtggct
    13321 aaaatgtctg gacgcggtct aggttacaca ggtggtacac tagacaagct agaagcaatt
    13381 cccggatttc agatcgagct ttcggatgaa gaatttgtac gcattgttaa tgaaagtaaa
    13441 gtagcggtaa ttggtcaatc aggaaacttg gcaccggcgg acaaaaaatt gtatgccttg
    13501 cgtgacgtca cagcgaccgt tgattctctt ccgctgattg ccagttcgat tatgagtaaa
    13561 aaaatcgcgg caggagcaga tgcaatcgtt ttggatgtga ccactggaga cggtgctttc
    13621 atgaagaaca tcgaagatgc aagacgtctg gcaaagacca tgacgagcat tgggaaactg
    13681 gctaaccgag aaacagtggc ggttatctca gatatgtcag aacctcttgg cgaagctatc
    13741 ggaaatagtc tagaagttgt cgaggcaatc gaaacattac aaggaaatgg tccagaagat
    13801 ctagtagaaa tgtgctacgc cttaggaagc caaatggttg ttcttgctgg caaagctaag
    13861 acaaccgatg aagcaagaac gttgcttcaa gaagctctgg aatcaggtaa agcgttagca
    13921 aaattcaaag aaatgatcca aaatcaaggg ggagacccag cgatcgtgga acatccagaa
    13981 cgaattttga ctgcacgcta cacaatggag cttcctgcta aacaaagcgg agttgtgtca
    14041 aaaatcgtgg caaatgaatt aggaattgct gcaatgatgc taggtgctgg gcgtaaaacc
    14101 aaagaagaag atattgatca cgctgttgga ttgaaactgc ataaaaaaat tggtgacact
    14161 gtgacaaaag gtgagtcact attgacgatc tatagtaacg acgaagaaat cacttctgtc
    14221 attgaattat tatataaaaa tattgaaatt ggtgaatcgg caatgaagcc aacgttgatt
    14281 catgacatca tcacagaata aaggaaaaaa gcggacttta ccgttttaaa gaaaagggga
    14341 aaagaaatgg aactaaaccg aatgatcgac catactattt tgaaagcaga cgcatctaaa
    14401 gaagctgtga tgcaaattat cgaagaagca aaaaaatacc atttttattc tgtctgcatc
    14461 aatccagcat gggttgcgct agcgaaagaa caattacaag gtgagccagt agccgtatgt
    14521 acagtaatcg ggtttcctct aggagcaaac acacccgaga caaaagcatt tgaaacaaca
    14581 gatgccatca ataacggtgc agatgaagtc gatatggtca tcaatatcgg tgctttgaaa
    14641 tcaaaggatg atcaacaagt ccaaaaagat atcgaagcag ttgttgaagc agcaaaagat
    14701 cgtgcgcttg tcaaagttat tattgaaaca tcgttattag atagagaaga aatcatccga
    14761 gcttgcgaaa tagcaaaagc tgcaggcgct gattttgtca aaacatcaac tggtttctca
    14821 acaggcggag caaaggtaga ggatgtccgt ttgatgcgtg aaactgtagg accagaaatg
    14881 ggtgtcaaag cttccggcgg tatccataac gaagaagaag caatggcaat gatcgaagct
    14941 ggtgctacaa gaatcggaac aagcgcagga gtagcaatcg tatctggttc aactggtgaa
    15001 ggctattaat tttttcaaag gagaagctca tggcagaact caaacaagaa tggatcaaca
    15061 cagctattga agcgttagat aaagcctatg ttccttattc ccattttccg gtaggcgctt
    15121 gtctcgtaac ggaatcaggc aagatttatc aaggcatcaa cattgagaat gcatcatttg
    15181 gattaacgaa ttgtgccgag cgcacagctt ttttcaaagc agtatctgaa ggagaaaaaa
    15241 catttacgca tctagtcgta gcggggcaca cccccgatcc tatctctcct tgtggtgcat
    15301 gtcgacaagt aatggcagaa ttctgttccc ctgatatgcc ggttacgcta gtaggagaca
    15361 atggtgtgac gaaggcaacg accgttcgtg cactgctgcc ttatgcattt actgaaaaag
    15421 atctataact catgttcatt cagtcagctg actgttgaaa aataaaacaa ttattttagg
    15481 aggctttaca gaaatgaaaa aagcaaaatt atttggttta ggtgctgtcg cactagcagc
    15541 agggttattt ttaggtgcat gcggcaacaa cggctctact gattcatccg gtggcaaaag
    15601 ctcatcagac acaacaactg cagcattgat cacagacaca ggtggtgtag acgaccgttc
    15661 attcaaccag tcagcttggg aaggactaga aaaatggggg aaagatcaag gactttcacg
    15721 aggaaatgat ggcttccaat atttccaatc ttcaaatgaa tcagattaca tcccaaatat
    15781 tgaccaagca ttgaatgctg gattcaaaac gatttttggt attggttata aactaaaacc
    15841 agcgatccaa gaacaagcaa caaacaatac aggtacgaac tttgttatta tcgatgatgt
    15901 catcgatgga ttagataacg ttgtatctgc aactttcaaa gacaacgaag cttcttatct
    15961 tgcaggtgtt gcagcagctt atacgactga aacaaacgta gtcggcttta tcggcggtgt
    16021 aaaaggtgaa gtaatcgatc gttttgatgc tggattcaaa gccggtgttg atgcaggtgc
    16081 gaaagaatta ggaaaagaaa tcaaagtatt gaatcaatat gctggtgact tctctgctcc
    16141 agataaagga cgttcaattg ctcaaggtat gtatgcacaa aacgcagata tcatcttcca
    16201 tgcttcaggc ggtacaggta acggtgtatt ccaagaagca aaatcattga acgaatcagg
    16261 cgacaaaaaa gtttgggtta tcggtgttga ccgcgaccaa tcagacgaag gcgaatacac
    16321 attaaacggc gaaaagaaaa acttcacatt gacatctaca ctgaaagcag ttggaacagt
    16381 agtagaagat ttggcacaaa aatcagcgga cggtaaattc cctggcggcg aacacactgt
    16441 ttatggattg aaagaagacg gtgttggtct aacagaagga caactttctg acgaagcgaa
    16501 aaaagcagtt gatgaagcaa aagaaaaaat catttcaggt gacgtaaaag ttccagaaac
    16561 tcctgaagaa aactaaaacc atttctatag ccttgttaga cacatgtttg acaaggctat
    16621 ggtttgatta gaacaagaat tgttcattta tccaagctgc cttgtgacag tttggataag
    16681 ggaacaactc cctgaagtaa tgtttgtttc aaaagaagaa cgctcactaa gcgtgaatct
    16741 tagtcaatcg ttgccctaga tagaaatcgt taggatgtga aaaaaaggtg gttcaagaaa
    16801 atattgtcat agaaatgcag aatattacca aagaatttgg aacattcaag gcaaatgaca
    16861 acatcaattt acaagtaaag gcaggagaaa ttcatgcgtt gcttggagaa aacggtgctg
    16921 gcaaatctac attgatgaac gtgctttccg gattattaga gccgacatca gggaaaattt
    16981 tgatgcgtgg gaaagaagta aagatcacaa gtccgaccaa agccaaccaa ttagggattg
    17041 ggatggtcca tcagcacttt atgcttgttg atgcctttac tgtaacagaa aacatcgtgt
    17101 tgggaagcga gcctagtcgc gcagggatgc ttgaccataa aaaagcacga aaagagatcc
    17161 aaaaagtttc tgaacagtat ggattgtcag tcaacccaga tgcttacgtt cgtgatattt
    17221 cagttggaat ggaacaacgg gtagaaattt taaaaacact ttaccgagga gcagatgtac
    17281 tgatttttga tgagccgaca gctgtattga cccctcaaga aattgatgaa ttaatcgtga
    17341 tcatgaagga attagtcaaa gaaggcaagt cgatcatttt gattacgcat aagttagatg
    17401 aaatcaaagc agtagctgac cgctgtacag ttatccgccg tggaaaagga atcggtacag
    17461 tcaacgttaa agacgtaacc tcacaacaat tagccgatat gatggttgga agaacggttt
    17521 cattcaaaac gatgaaaaaa gaagcgaagc ctcaagaagt cgttttgtct attgaaaatc
    17581 tagtggtgaa agaaaatcgt ggattagaag ccgtgaaaaa cttgaactta gaggttcgtg
    17641 ctggcgaagt acttggtatc gctggaatcg atggaaacgg gcagtcggag ttgatccaag
    17701 ctttgactgg tttgcgaaag gcagaaagcg gacatatcaa gctaaaaggg gaagacatca
    17761 ctaataaaaa accccgaaaa atcactgaac atggtgtagg acacgtgcca gaagaccgtc
    17821 ataaatacgg gttagtccta gatatgacat tatctgaaaa tatcgcacta caaacctatc
    17881 atcaaaaacc ttacagtaaa aacggcatgc tgaattattc agtaatgaat gaacatgcca
    17941 gagaattgat cgaagaatat gatgttcgaa caacgaatga acttgttccc gcaaaagctt
    18001 tatcaggcgg aaatcagcaa aaagcaatta tcgctcgtga agtcgaccga gatcctgatc
    18061 tgttgattgt tgcaaatcca actcgtggac tggatgtagg tgcgatcgaa tttattcata
    18121 aacgtctgat cgaacaaagg gacaaataca aagcagtgtt actgatcagt ttcgaattag
    18181 aagaaatttt aaatgtttcg gatcgtattg ctgttatcca tgaaggagaa attgtcggta
    18241 tcgttgatcc gaaagaaaca tctgaaaatg aattaggtct attgatggcg ggctatacct
    18301 tagaagaagc acgaaaagag ttagaaaagg gggctggcgc acatgaatga tcgcagcgaa
    18361 aaattccgta atttattagt gcctgtactt tcagttgttt taggattgat cttaggcgcg
    18421 atattgatgg ctgcttttgg ttttcagccc atccaaggtt attcttctat gctagatgca
    18481 gcccttggtt cgcaaagagg gataggtgaa acgttaaggc aagcaacgcc gttgattttt
    18541 acagcattag gtttttctgt agctaactca gctgggtttt tcaatattgg gctttctgga
    18601 caagcacttt gcgggtggat cgtcagtgtc tgggtggcac ttgcgtttcc agatctgcca
    18661 aaagttgttt tgcttccgtt atgcgtgatc ttaggtgcct tagctggtgc gatggcagcg
    18721 gctgttccag gtttacttcg agcatttttt ggaacaagtg aagtgatcgt aacaatcatg
    18781 atgaactata tcttattata cgtaagcaca tttgctttac atgaagtgat gcctgaaagc
    18841 tatcgctcaa gtttggactc ttccaatatg atcagtcaaa atgcctcact gcggttacca
    18901 tggttaacac aagcttttgg cggctctcgc gtaaactcag gattattttt agcaatcatc
    18961 gcgcttattt tcgtttggct gctgatgaag aaaacgactt taggatttga aatccgttct
    19021 gttggattga atccttttgc ttctgagtat gcaggaatga gtagtaaacg tacgatcgtt
    19081 ttgtcgatgg tcatttctgg cgctttggca gggcttggcg gtgtggttga aggattagga
    19141 acgtatcaaa acttctttgt tcaaacgact tcgctatcta ttggatttga cggaatggct
    19201 gtttccttgc ttggttctgg atcagcaatc ggtattcttt tatcagcgat cttgttcagt
    19261 attttgaaga tcggtggact tggcatgcag acaggagcag gtgttccgtt tgagatcgtc
    19321 aatgtatcga ttgctttgat tatcttcttt gtcgggatca acttcctgat tcgtttcgtc
    19381 atggctaaag tcttccaagg gaaaaaacaa gaagagatcg ttgcgacaat agaatcgaaa
    19441 ccaacgaatc aaaaccatga agggggcgaa ctataatgtc gactatcgaa ttaattgcct
    19501 tgatccttac ttctacactg gtttattcaa caccgctaat tctaacgtca ctaggcggaa
    19561 ctttttctga acgaagcggg atcgtaaacg tagggctaga gggaatcatg gtcatgggtg
    19621 cttttagttc catcgttttc aacttgacga tgagtccatc acttggttct atgacaccat
    19681 ggatcgctat tttagtgggt ggcgtcgtag gtatcctatt ttctctgatt cacgcagtag
    19741 caacagtcag tttgcgtgct gaccatatta tcagcgggac agtaattaat ctgatggcac
    19801 ctgctttagg tgttttcttg atcaaagcat ggtatggtaa aggtcaaact gataacatca
    19861 cgcagaatct ggggtatttt tctttcccag gattagcaga tattccggtc attggaccaa
    19921 ttttctttaa aaatacattc ttacctgctt attgtgctat tttgattgct gtcctagctt
    19981 gggtgatcat cttcaagacg aaattcggct tgcgcctccg ttcagttggt gaaaatcctc
    20041 aagctgccga cacattaggt atcaatgtat atgctatgcg ttattcaggc gtactgattt
    20101 ctggcttgtt aggcggtatg ggcggtgctg tttttgctca gtcgatttcc ggtaactttt
    20161 cagctggtac gatcgttgga caagggttca tctccatggc tgctatgatc tttggtaagt
    20221 ggaatccttt aggggcaatg ggtgcctcat tgttctttgg ttttgctcaa agcttgagta
    20281 tcattggcgc acaattacca gttatttctt cgattccgcc agtattgctg caaattgctc
    20341 catatttgct gacaatcatt gtgctggttg ttttcttagg gaaagcttct ggtccaaaag
    20401 caaacgggaa gaactatatc aaatcaaaat aatcttttag cgaataaaaa gggtgggtta
    20461 cgggaagtgt ttttcgtctc gccctttttg ttttaaaggc aataaaaata aaaagaaaga
    20521 aggaataatc atgtttaaac gcgtacatct gatcgtcatg gattcagtag gaattggaga
    20581 agcaccagat gccgataagt tcggtgatgt tggttctgat acattaggac acattgcgaa
    20641 agaagcagga ttgaacgttc cacatttaga gcagcttggt ttaggaacga tccgtcctct
    20701 tgaaggtgtg gaatcagtga aagatcatca aggctatgcg acgaaattag aagaagtttc
    20761 tgtagggaaa gatacgatga caggccactg ggaaatcatg ggattgaata tcaaaactcc
    20821 atttcgtgtg tttcctaatg gattccctga tgatctgatc aaacaaatcg aagaattttc
    20881 tggccgtaaa gtcgtatgta acaaacctta tagcggaaca gctgttattg acgattacgg
    20941 tgaacaccaa atgaaaacag gcgatttaat tgtatatact tctgccgatc ctgtccttca
    21001 gatcgcggca catgaagaaa tcattccttt agaagaatta tatcggattt gccaattcgt
    21061 tcgtgatata acaaaagatg atccgtatat gattggccga atcattgcac gtccgtacgt
    21121 gggtgaacca ggagacttca cacgaacaag caatcgccat gattatgcgt tgaatccatt
    21181 cggacacaca gtccttgatt ctttgaaaga agcaggaaag gacgtgattg ctgttggaaa
    21241 gatcaacgat attttcaacg gacaaggaat cactgaatct gtccgcacga aaagcaatat
    21301 ggatggcgtt gaccagctgc tgaaagttat gaaaaacgat ttttcaggaa tcagctttac
    21361 caatttagta gattttgatg cattatacgg tcatcgtcgg gatgtagtcg gctatgcgca
    21421 tgcgattgaa gaatttgact tacgaattcc agaattgctt gaagcgatgg aagaagacga
    21481 cttgctaatg attacagccg atcacggaaa cgatccgacg tttactggta ccgatcatac
    21541 gcgagaatat gtaccgttat tggtttacag taaaaaaatg aaagaacaag gggcattgcc
    21601 tcaaggatat tattcagata tttcagcaac gattgctgag aactttagtg tttcttcgac
    21661 tgaaaatggc gaaagcttct tgaaattatt aaaataatct ttttaaatag gagaaagcaa
    21721 atgaccaagt taagcgagat gttacaagaa acgacccagt ttatccttga taaaggagta
    21781 aaagaaattg attttggttt gattctaggt tcaggattag gtgaattagc agatgaaatc
    21841 gaagatgctg taatgatttc ttatgatgag atcccgttct ttcctgtttc gacagtagtt
    21901 gggcatgcag gaaagctagt ttatgggtct ctggcaggca agaaagtgtt agccatgcaa
    21961 gggagattcc actactatga aggtcattcc atgcaaacag tgacctatcc agtacgtgtg
    22021 atggccggac taggggccca ttcagtcatt gtgactaatg catgtggtgg tgtcaacgaa
    22081 tcatttgtac caggagattt gatgctgatt accgatcata tcaattttac tggtgaaaat
    22141 cctttgatcg gtccgaatga agatgcaatg ggccctagat ttccagatat gagtcaagct
    22201 tatacgcctg actatcaagc aacagcaaaa gcagcagcag aaaaactagg tttggatctt
    22261 aaagaaggcg tatatatggg ctattctgga ccaacttatg aaacgcctgc ggagatccga
    22321 atgtctcgga caataggagc cgatgcagta gggatgtcga ctgttccaga agtcatcgta
    22381 gcagctcaca gcggattaaa agtgttagga atctcttgta ttaccaactt agcagcaggt
    22441 atgcaagcga atctgagcca tgaagaagtt gtagaaacaa ctcagagagt caaacaatct
    22501 tttaaagcat tggttaaaga aacattgaca ttattgtaat aggaggaaca ataaaatgag
    22561 tgtgcatatt gaagcgaaag aaggcgagat cgccgataag attttacttc cgggagatcc
    22621 tttgcgagct aaatatattg cggagacttt tttggaagat ccagtttgtt acaatcaggt
    22681 acgcggtatg ttaggcttta ccggcaaata taaaggagaa cgtatttctg ttcaaggaac
    22741 aggaatggga atgccatcag ccaccatcta tgcccatgaa ttgatccagt cgtatggtgt
    22801 aaagaaattg attcgagtag gtacatgcgg tgcattgtca aaagacgtcc atgttcgtga
    22861 tttagtacta gcacaaggtg cagcgaccag ttcttcgatg atcgaaaaaa atttccaagc
    22921 ctttcatttt cctccaatca gtgatttcaa tctattactg aaagcatatg agatagctaa
    22981 agaaaagggc tatacggttc atgtaggaaa tgttctttcg gaagattctt tctacaaaga
    23041 tgatttgaca gagacgttcc aattagctga acttggcgtt ttaggagtgg aaatggaagc
    23101 agcggctctt tattacttag gcgcgaaata ccatgtgcag acattaagtt tgatgaccgt
    23161 tagcgaccat ttgatcaccg gtgaagaaac aacagctgca gaacgtcaat ctacgttcaa
    23221 tgaaatgatc gaagtaggat tggaaacagc gatagcagaa taagaaggaa atgaaaaagc
    23281 tgccctgcat taagtaataa gaacatccaa gcaaaaatag ctcctcatgt ttttgacttg
    23341 gctggactta ttattgcaaa tgtaagcttt tgaacaccgt ttaccaagag gttgtgaatc
    23401 tgccgtttaa ctctgagtca caaggaacaa tttcaaaaaa cagcttttca tgtttgatga
    23461 atattgtgac ttgtgaccga gaagttggca ggagaaaacc gtttatacga aaaaagggac
    23521 tgtgacaaat cgtgtcacag tccctttttt atagtgtttt ttataggtag tattttttag
    23581 caactgttaa gtcatcattt aattcataaa caagtggttg acctgttgga atttcaagat
    23641 ccatgatgtc ttcgtcagag ataccttcga tatgtttagc tagggcgcgt aatgagttac
    23701 catgtgcagc tactaaaaca gttttgttgt ctagtaatgc tggagcaatt tcatcttgcc
    23761 agaatggcaa agcgcgttct aatgtcactt tcaagttttc tccaccagga atatcgcgtt
    23821 tgtctaacat tgcgtaacga cggtcatttg ctgcagaacc ttcgtcagtt tcttccatta
    23881 atggaggaag agtatcgtaa gaacgacgcc agatgtgcac ttgttcgtcg ccgtattttt
    23941 cagctgtttc ttttttgttc aaaccttgta atttaccata gtgacgttcg tttaagcgcc
    24001 atgatttgat ttgtggtacc caaagttgat ctgaatattc taagatcaag ttacatgttt
    24061 tgattgcacg agttaaaaca gaagtgtaag caacatcaaa ttcgattcca gcttctttga
    24121 ttttgcgtcc gccttctttt gcttcttcga ttccttctgg tgctagattt acatcagccc
    24181 aaccagtaaa ttggttcaat gcattccatt cactaagacc gtgacgagaa aaaactaatt
    24241 ttggcatgtt tagctctcct tttaagatag gtttatattt ctgaaatttt cagttctgtt
    24301 ttattgtaca attttttgga agagatttcc atcgaaaaat tagataagat tctatgagaa
    24361 aacaagtaga gaatataaaa aaacgaacag tgcccacgca actgttcgtt tttaggtatt
    24421 agatgcataa ttgctcgatt tctttttcag gtaaattgat atatttataa catgttccaa
    24481 ctgaaacacc gcattttgaa gaaataaatt ggatcgtttc ttttttttca taatagagac
    24541 gtcggatttt tttgatcgtt tttgcatcga tttttggtct tccgccgatt tttccttttt
    24601 tacgagcttc atctaatcca actaatgtac gttctttgat caatgcgcac tccatgtttg
    24661 ctaggttttc cattaattgg aaatagattt ttcccatttc gcgcgtatcg attccttcat
    24721 caagactaat caagtgcagc ccgcgatctt tgaataattg cgtgagttct gttaattgcc
    24781 gcgtagattt gcctaatcga tgaagttggc aaatgactag tgcatcgcct tgttgcatgt
    24841 cagcgatcac tgactccaaa tgaacagcag tagaatcatt ttgttccact tcatatgtct
    24901 catggaaaat ttcttgacaa ccaaagtcag ataattgatc aacctgagtt gttaaatcaa
    24961 cgtcatttat ggttgtgcgt gcataaccaa taattttcat ggtgattcct ttctagcagt
    25021 gattttctaa ctggatataa cagtaaaaat gtagcataaa gtacgtcaaa aagatagttt
    25081 tttcgcaaat tttccgctgt ctttttttat tggatgatca agcagttttt ctttatttac
    25141 gacggattgc gtcaagaatc tcaacgcctt tttctgagcc gacaactact ttagaaacca
    25201 tgtttaggaa aagcccatgt tctactaccc caacaagttc atctaaatag gtagctaagc
    25261 tttctggctg ttcgatgact tctagatgca gatcaatgat gtaatgacca ccatctgtgg
    25321 ttaagacttc tccgttttcg tctgtacgca agacgggttg gaatcttttt tcttcaaaaa
    25381 tatgaagtag ctgttgggag ccataaggga tcacttcaac tgggagtggg aacttcccaa
    25441 gtttatcgac taatttagaa gaatcaacga tccaaatcgt ttcttttgag tacgtagcga
    25501 ctattttctc gaaaagcaaa gcagcgccgc cgcctttgat cccttgaaaa tcagcactga
    25561 tttcatcagc accgtctatt gtcaaatcga ctacggggac ttcatcaata ctctttaacg
    25621 gtattcctaa tttttccgct tgttcttttg tcgcatttga agtagtaact cctgtgatac
    25681 gtaaaccttc ttcacgcata cggcgaccaa tctcttctac catataatag gcggttgaac
    25741 cggtcccgag acctacgatc atcccgtcct ttacgaattt cgcggcttca atgccagcta
    25801 attgttttaa attcatcttt gcgtccacct ttttttagtt tgagcatcgt gctttcctca
    25861 ctattttagc gtgatttacg gtaaaaagat accattcttt tttattccaa taaagctttt
    25921 tcaaaaaaaa ttgcatctct tgttgacatg ttttttcatt cgtgatattc taacaaaggt
    25981 gctttatcta caggtttctg tgaagaaacg tgctgactgt ttatcaaagc atatttgata
    26041 aggatgcaga cattgcattt attttttatc acaaaaaaag aaccacctgg atgtgtggaa
    26101 ctagacgcaa atcgtgggaa ggaggaaaca aggaatgcct acaattaacc aattagtacg
    26161 taaacctcgt aaatcaaagg tggaaaaatc taattcacct gctttgaaca aaggatataa
    26221 cagctttaag aaaactcaaa ctaacgtaaa ctcacctcaa aaacgtgggg tagcaactcg
    26281 tgtgggtaca atgacaccta aaaaaccgaa ctcagcttta cgtaaatatg cacgtgttcg
    26341 tttgtcaaac ttaattgaag ttactgctta tatcccaggg attggtcata acttacaaga
    26401 acacagtgtt gtattattac gcggtggtcg tgtaaaagac ttaccagggg tacgttacca
    26461 tatcgttcgc ggtgctttag atactgctgg tgttaacgac cgtaaacaaa gccgttctaa
    26521 atacggaact aaaaaaccta aagcttaatt ttaattatta aacaaatgaa acttgcttct
    26581 actatataga agtaattact atgaacaata tttcggaagg aggagttacg gatgccacgt
    26641 aaaggtcctg ttgcaaaacg tgatgtttta ccagatccta tttataactc aaaattagta
    26701 actcgcttga tcaaccgtgt aatggttgac ggaaaacgcg ggattgcagc taacattatc
    26761 tataatgcct ttgacttgat caaagaatct acaggtaacg atccattaga agtgtttgaa
    26821 caagcaatga aaaacgttca acctgttctt gaagttaaag cacgtcgtgt tgggggttca
    26881 aactaccaag taccagttga agttcgccct gaacgccgcg ttactctagg tcttcgttgg
    26941 gtagtaaact acgctcgctt gcgcggtgaa cacacaatgg aagaacgctt agcaaaagaa
    27001 atcatggatg ctgcaaacaa cacaggtgct tctgttaaaa aacgtgaaga cactcataaa
    27061 atggctgatg ccaaccgtgc gtttgctcac tatcgctggt aagattctct ctgtttggtc
    27121 ggcttttata agtcgacctc agatagtgtc cttattttta gcaactagat agtaaagaga
    27181 ggagaaacaa ggagaatgac cagagaattt tctttacaaa acactcgtaa tatcggtatc
    27241 atggcccaca tcgatgctgg taaaacgact gctactgaac gtatcttgta ctatactggt
    27301 aaaatccata aaattggtga aactcatgaa ggagcttcac aaatggactg gatggaacaa
    27361 gaacaagaac gtggtattac gatcacttct gctgctacta cagcacaatg gaagggtcac
    27421 cgaatcaaca tcatcgacac accaggacac gtagacttca cagttgaagt tgaacgttca
    27481 ttgcgtgtac ttgatggtgc tgtaactgtt cttgatgcac aatcaggtgt tgaacctcaa
    27541 actgaaacag tttggcgtca agcaacaact tatggtgtac ctcgtatcgt atttgcaaac
    27601 aaaatggaca agatcggtgc tgacttctta tattcagtat caactttaca tgaccgtttg
    27661 caagcaaacg ctcacccaat ccaattacca attggtgcgg aagacgactt cacaggaatt
    27721 atcgaccttg taacaatgaa ggctgaaatg tatacaaacg atcttggaac agagatcgaa
    27781 gaaacagaaa ttcctgaaga ataccgcgaa ttagctgaag aatggcgcga aaaattggtt
    27841 gaagctgtag ctgaaactga tgaagaactt actttgaaat acttagaagg tgaagaaatc
    27901 actgaagctg agttaaaaga aggtatccgc cgcgcaacag ttaacgtaga attctaccca
    27961 gtactatgtg gttcagcatt taagaacaaa ggggttcaat tactattgga cgctgttctt
    28021 gattacttgc catcaccgtt agatatccca gctatcaaag ggattgaccc taaaactgat
    28081 gaagaagtag aacgtcctgc agacgacagc gctcctttct cagctttagc atttaaagtt
    28141 atgactgacc cattcgtagg tcgtttaact ttcttccgtg tgtactctgg tgttttacaa
    28201 tcaggttcat acgtacaaaa cgcaacaaaa ggcaaacgtg aacgtgttgg tcgtatccta
    28261 cagatgcatg ccaactctcg ttcagaaatc tctgaagttt acgctggaga tatcgctgct
    28321 gctgttggtt tgaaagacac aactactggg gacacactat gtgatgaaaa gagtctagtt
    28381 attcttgaat ccatggaatt cccagaacca gttatccaag tcgctattga acctaaatca
    28441 aaagctgacc aagataaaat gggtgtggct ttacaaaaac tatctgaaga agatcctact
    28501 ttccgtgctg aaacaaacgt ggaaacaggc gaaacaatca tcgctggtat gggtgaactt
    28561 cacttagata tcatcgttga ccgtatgcgt cgtgaattca aagttgaagc taacgtaggt
    28621 gcgcctcaag tatcttaccg tgaaactttc cgtgctggca ctcaagccga aggtaagttt
    28681 gtacgtcagt ctggtggtaa aggtcaatac ggtcacgtat ggattgaatt tacaccaaat
    28741 gaagaaggtg ctggtttcga atttgaaaat gcgatcgtcg gtggggttgt tcctcgtgaa
    28801 tacattccag ctgttgaaac tggtttgaaa gatgctatgg aaaatggtgt cttagctggc
    28861 tatccattag tagatatcaa agctaaactt tacgatggtt cataccatga tgtcgattca
    28921 aatgaaactg ccttccgtgt tgctgcttct atggcattac gtgcagcagc taagaaagct
    28981 aatccagtaa tccttgaacc aatcatggct gtagaagttg ttatccctga agattattta
    29041 ggtgatgtta tgggtcacgt aactgctcgt cgtggacgtg ttgaaggtat ggaagctcgc
    29101 gctggcggcc aacaagttgt tcgtgcgatg gttccacttg ctgaaatgtt tggttatgct
    29161 acaactcttc gttctgctac tcaaggtcgt ggtacattta caatgacatt tgaccactac
    29221 gaagatgtac caaaatcagt acaagaagaa atcattaaga aaaacggcgg aaaagctgaa
    29281 taatttcttg aaaccggata gtcctgcaaa gagattatcc aaagagacta tatttttaat
    29341 atagtttcgt gtaaaataat tatcgatgat atgggcatgt ggtgtacctt gtgtacatca
    29401 tatacctatc agacaaataa aaaatatatt gtatttttag gaggacattt taaaatggca
    29461 aaagaaaaat ttgaccgttc taaaccacac gttaacattg gtactatcgg acacgttgac
    29521 catggtaaaa ctacattaac agctgcaatc acaactgtac tatctaagaa aaacggcggc
    29581 caagctatgg cttacgatca aatcgatggt gctccagaag aacgcgaacg cggaatcaca
    29641 atctcaacag ctcacgttga gtatgaaaca gatacacgcc actacgctca cgttgactgc
    29701 ccaggacacg cggattacgt taaaaacatg atcactggtg ctgctcaaat ggacggagct
    29761 atcttggtag tttctgctgc tgacggccca atgcctcaaa ctcgtgaaca catcctattg
    29821 tctcgtcaag ttggtgttcc ttacatcgtt gtattcttga acaaagtaga catggttgat
    29881 gacgaagaat tactagaatt agttgaaatg gaagttcgtg acctattaac agaatacgaa
    29941 ttccctggtg acgatgttcc tgtagttgct ggatcagctt tgaaagctct agaaggcgac
    30001 gcttcatacg aagaaaaaat tcttgaatta atggctgcag ttgacgaata catcccaact
    30061 ccagaacgtg acaacgacaa accattcatg atgccagttg aagacgtgtt ctcaattact
    30121 ggacgtggta ctgttgctac aggtcgtgtt gaacgtggac aagttcgcgt tggtgacgaa
    30181 gttgaagttg ttggtattgc tgaagaaact tcaaaaacaa cagttactgg tgttgaaatg
    30241 ttccgtaaat tgttagacta cgctgaagct ggagacaaca ttggtgcttt actacgtggt
    30301 gttgcacgtg aagacatcca acgtggacaa gttttagcta aaccaggtac aatcacacct
    30361 catacaaaat tctctgcaga agtatacgtg ttgacaaaag aagaaggtgg acgtcatact
    30421 ccattcttca ctaactaccg tccacaattc tacttccgta caactgacgt aacaggtgtt
    30481 gttgaattac cagaaggaac tgaaatggtt atgcctggcg acaacgtaac aatggaagtt
    30541 gaattaatcc acccaatcgc tatcgaaaac ggtactaaat tctcaatccg tgaaggcgga
    30601 cgtacagttg gtgccggtgt cgttacagaa atcaaagctt aattcactta agttttaaag
    30661 taaagcaaca tttattcgga cgtaatcgct cggaacgaag gtcttaggat ctttgttccg
    30721 agctttttta tttattgcag gatcaggaaa aaagacagga gtattataaa tagaagaaac
    30781 acgaaataaa agagtcagta ttttctaaat gaaactttat gaaaacaaaa tgttttttac
    30841 aagaaaaagc cttgctttgt gagtttatat atattataat gacaaaggtg cgtttacttt
    30901 agtgaattgc acagacgtaa tcagaatgat ttcgtcggct aagaaacgag aggttgcgac
    30961 acgcccggac gctttgccac gaacgagcgt gacggaaatt ttcgtggagc tatgtctact
    31021 tttcaaaata ggcgaaggag ggaacaagat ggcaaaacaa aaaattcgta tccgtttaaa
    31081 agcgtatgaa caccgtattt tagatcaatc agcggataaa atcgtggaaa ctgcaaaaag
    31141 aactggagct agcgtgtctg gtccaattcc attaccaact gaacgcagtc tttacacaat
    31201 catccgtgcg actcataaat acaaagattc acgcgaacaa ttcgaaatgc gtacacacaa
    31261 acgtcttatc gacattgtga acccaacacc aaaaactgtt gatgctttga tgaagctaga
    31321 cttaccatct ggtgtaaaca tcgaaatcaa attataaaac taaaacaaaa ataatggagg
    31381 tgtactcatg accaaaggaa tcttagggaa aaaagtggga atgacacaaa tcttcactga
    31441 atctggtgaa ttaattccag taactgttgt agaagctact ccaaacgtag tactacaagt
    31501 gaaaacaatg gaaacggatg gctacgaagc tattcaagtt ggttaccaag atatgcgtga
    31561 agttttatca aacaaacctg cgaaaggtca tgttgcaaaa gcaaacacgg ctcctaagcg
    31621 cttcattcgt gaattcaaaa atgttgagct aggagaatat gaagtaggaa aagaaattac
    31681 agtagatgtt ttccaagcag gagacattat tgatgtaaca ggtactacga aaggtaaagg
    31741 attccaaggg gttatcaaac gccacggaca aagccgcgga ccaatggctc acggttctcg
    31801 ttaccatcgt cgtcctgggt caatgggtcc agttgcacct aaccgtgtat ttaaaaacaa
    31861 acgtcttgct ggccgtatgg gtggcaaccg cgtaactatc caaaacttgg aagttgtacg
    31921 tgtcgatgca gaaagaaatg ttatcttgat caaaggtaat gttcctggag ctaaaaaatc
    31981 tttaattact atcaaatcag ctgtgaaagc taaataattg taggaagaaa ggaggaacta
    32041 aggaatgccg aatgtagcat tattcaaaca agatggaagc caaaacggtg aaatcacact
    32101 taacgaagaa atcttcggaa ttgaacctaa tgaaagtgtt gtttacgatg caatcgtaat
    32161 gcaacgtgcg tcattaagac aaggaacaca cgcggttaaa aaccgtagcg ctgttcgcgg
    32221 cggcggacgt aaaccatggc gtcaaaaagg aacaggtcgt gcacgtcaag gatctatccg
    32281 ctcaccacaa tggcgtggag gtggcgtagt ctttggacca acaccacgtt cttacagcta
    32341 caaacttcct aaaaaagttc gtcgtttagc aatgaaatct gtattgtcag aaaaagttgc
    32401 tgaaaacaac ttggtagcaa tcgaaggctt gaacttcgac gcaccaaaaa caaaagaatt
    32461 caaacaagtt cttgctaact tatctatcga ctctaaagta ttggttgttc tagaaaaagg
    32521 taacgacttt gctgctttat cagcacgtaa cttaccaaac gtttctgtag taacttctga
    32581 taatgtgagt gtgctagacg ttgtatcaaa tacaaaagtg ttagcaacac aaactgctct
    32641 tactcaaatt gaggaggtgc ttgcataatg aacttactag acgtaatcaa acgcccagtg
    32701 atcactgaaa aatcaatgct tgccatggat gacaagaaat acacttttga agtggacact
    32761 cgcgcaaaca aaactttagt aaaacaagcg gtagaagctg cttttgacgt taaagtcaaa
    32821 aacgtgaaca tcgtgaacgt acgtgcgaaa ttcaaacgta tgggcaaata tgcaggatat
    32881 acaaaaaaac gtcgcaaagc gatcgttaca ttgactgaag attcaaaaga aatccaatta
    32941 ttcgaagctg ctgaataagc actaatagat aactcattaa attaggaggg aaaaacgtgg
    33001 cgattaaaaa gtacaaacct acctcaaatg gtcgtcgtaa catgacaggt tctgattttg
    33061 ctgaaatcac aacatcaaca cctgaaaaat cattgttaca accattgaaa aaccatgctg
    33121 gacgtaacaa caacggccgc atcactgtac gtcatcaagg tggcggacac aaacgccaat
    33181 atcgcgtgat cgacttcaaa cgtaacaaag ataacgttgt tgcaactgtt caaacgatcg
    33241 aatatgatcc aaaccgttca gctaacatcg cgttagtaca ctatgaagat ggagtgaaag
    33301 cttacatctt agcacctaaa ggactagaag tgggtatgaa acttgtttca ggtccagaag
    33361 cagatatcaa agtcggaaat gctcttccat tagaaaacat tccagttggt actgttatcc
    33421 acaacattga aatgaaacct ggtaaaggtg gacaattgat ccgttctgct ggtacaagtg
    33481 ctcaagtact tggtaaagaa ggcaaatacg tattgatccg cttgaactct ggtgaagttc
    33541 gtatgatctt agctgcatgc cgtgctacaa tcggttctgt aggtaacgaa caacacgaat
    33601 taatcaacat cggtaaagct ggacgctctc gttggatgcg taaacgccca actgtacgtg
    33661 gtagcgtaat gaaccctaat gatcacccac acggtggtgg tgaaggtaaa gctcctatcg
    33721 gacgtaaagc accagtttct ccttggggtc aaccagctct tggttacaaa actcgtaaca
    33781 aaaaagctaa atcagacaaa cttattgttc gtcgtcgcaa aaataaataa ttactttgac
    33841 catgtgtcgc acattttgag aggaggttca ccatgggtcg tagtttaaag aaaggacctt
    33901 ttgtcgatga gcatttaatg aaaaaagttg aagctcaagc aggggttgaa aagaaaaaag
    33961 ttatcaaaac ttggtcacgc cgttcaacag ttttcccaag ttttgttgga tataccatcg
    34021 cagtttatga tggacgtaaa catgtaccag tttacatcca agaagacatg gtaggacaca
    34081 aattaggtga atttgcacct acaagaactt atcgtggaca tgctgctgac gataagaaaa
    34141 cgaaacgcta atttgagggg aggataaatc caaatggcag aacaaatcac atcagctaaa
    34201 gcaactgcaa aaacagttcg cacttcacct cgaaaagccc gtttagtaat tgaccttatc
    34261 agaggtaaaa gtgtcgcaga tgcaatttca atcttgaaat tcacaccaaa caaatctgct
    34321 ggaatcattg aaaaagtatt gatgtcagca gttgcgaatg cagaaaataa ctttgactta
    34381 gatgttgaaa acttggtagt aagcgaagct tttgttaacg aaggacctac aatgaaacgt
    34441 ttccgtcctc gtgcaaaagg atcagcttct ccaatcaaca aacgtacaag tcatatcacg
    34501 gtagtcgtat cagaaaaata aggagggata atcagtgggt caaaaagtac atccaattgg
    34561 aatgcgtgta ggcatcatcc gcgactggga tgctaaatgg tatgctgaaa aagagtatgc
    34621 tgagttctta cacgaagatt taagaatccg taaatttatt tcaactaaac ttgctgatgc
    34681 tgctgtatca acagttgaaa tcgaacgcgc aacaggtcgc gtaaacattt caattcacac
    34741 agctaaacca ggtatggtta ttggtaaagg cggttctgaa gtcgaaagcc taagaaaaga
    34801 attaaacaaa ttaactggta aaagaatcca tatcaatatc gtggaaatca aaaaaccaga
    34861 tctagatgca aaattagtag gtgaaggaat cgcacgtcaa ttagaaaacc gtgttgcttt
    34921 ccgtcgcgct caaaaacaag ccatccaacg ctcaatgcgt gctggcgctc aagggatcaa
    34981 aactcaagta tctggtcgtc taaacggagc agatatcgct cgttcagaag gatactcaga
    35041 aggaactgtt cctcttcata ctttacgtgc ggacattgat tacgcatggg aagaagcaga
    35101 tacaacatac ggaaaactag gagttaaagt gtggatctat cgtggagaaa ttcttccaac
    35161 taaaaaaaac actgagaaag gagggaaata atcatgttag tacctaaacg tgtaaaacac
    35221 cgtcgtgaat tccgcggaaa aatgcgcggg gaagctaaag gcggaaaaga agtagcattc
    35281 ggtgaatacg gtttgcaagc tgttgattca cattggatca caaaccgcca aatcgaagct
    35341 gctcgtatcg caatgactcg ttacatgaaa cgtggtggga aagtatggat taaaattttc
    35401 cctcacaaat cttatactgc caaagcaatt ggggtacgta tgggttctgg taaaggggca
    35461 cctgaaggat gggttgcacc agtaaaacgt ggtaaaatca tgtttgaaat cgcaggcgtt
    35521 cctgaagaag tagctcgtga agcgttacgt ctagcttctc acaaattacc aatgaaaact
    35581 aagatcgtaa aacgtgagga aatgggtggt gaatcgaatg aaggttaaag aaatcagaga
    35641 attaaccact gccgaaatgc ttgatcaaga aaaacaatta aaagaagaat tgtttaatct
    35701 tagattccaa ttagcaacag gtcaattaga aaacactgca cgtattaaag aagtacgtaa
    35761 atcgattgca cgcatcaaaa ctgtgttgcg tgaacaagct aagtaattat ggaaggaggc
    35821 cattaagcgt atgactgaag gaagaaatca acgcaaagtt tatcaaggtc gcgtggtttc
    35881 agacaaaatg gataaaacaa ttaccgttgc tgtcgaaaca aagaaaaacc accctatcta
    35941 tggcaaacgc atgaactatt ctaagaaata caaagtgcat gatgaaaaca acacagcaaa
    36001 agttggcgac atcgtgagaa ttatggaaac tcgtccatta tcagctacaa aacgtttccg
    36061 tttgttggaa gtagtcgaag aagcagttat tatctaataa cacgagattt tcctatatag
    36121 attgaatgca aaatctgaaa ggaggataca caacgtgatc caagcagaaa gtcgtttaaa
    36181 agtcgctgat aactcaggcg cacgtgaaat tttgacgatc aaagtccttg gtggatctgg
    36241 tcgtaaaact gctaacatcg gtgatgtgat cgttgctacg gttaaacaag caacgccagg
    36301 tggggttgtc aaaaaaggtg acgtcgttaa agccgttatc gttcgaacaa aatcaggagc
    36361 tcgtcgtaca gacggttcat atattaaatt tgatgaaaat gctgcagtga ttattcgtga
    36421 tgataaaagc cctcgtggaa cacgtatctt tgggccagtt gcacgtgaac tacgtgaaaa
    36481 caacttcatg aagatcgttt cactagcacc agaagtttta taatcttgag acacttatca
    36541 aaggaggtgc gaaacagtta tgtttgttaa aaaaggcgat aaagttaaag ttatcactgg
    36601 aaaagacaaa aacaaagaag gcgttgtact agcagcgttc ccgaaaaaag ataaagttat
    36661 tgtcgaaggt gtaaatgtcg ttaaaaaaca ccaaaaacct tctcaagctg ctcctcaagg
    36721 tggaattgtt gagatggaag cgccaattca tgtttctaac gtgatggtgg ttgactctac
    36781 tggtgtagct ggccgagttg gctacaaaga agtagacggc aaaaaagtcc gtgtttctaa
    36841 aaaaaccggt gaagttttag ataaataaaa gtttaaggaa ggagggtctt actgaatgaa
    36901 ccgcctaaaa gaaaaatatc ttaaagaagt aactccatca ttgatggaaa aatttaacta
    36961 tagctctgtt atgcaaacac ctaaagttga aaaaatcgtc atcaacatgg gtgttggtga
    37021 tgcggtatca aacgctaaaa acttagataa agcagttgaa gaattaactt tgatcactgg
    37081 tcaaaaacca atgattacaa aagctaaaaa atcaatcgct ggattccgtt tacgtgaagg
    37141 aatgccaatt ggtgcaaaag ttacattgcg cggagaaaga atgtacgaat tcttagacaa
    37201 attggtttct gtttcactac cacgtgtacg tgacttccat ggtgtaagta aaaaagcctt
    37261 tgacggtcgt ggaaactata ctttaggtat taaagaacaa ttgatcttcc cagaagtaga
    37321 ttatgattta gtagataaag tacgtggtat ggacatcgtt attgtaacaa cagcaaactc
    37381 tgatgaagaa tctcgtgagt tgttgacaca attaggcatg ccattccaaa aataattaaa
    37441 ggaggcgaac tacgtggcta aaaaatcaat gattgctaaa aacaaacgtc ctgcaaaatt
    37501 ttcaacacaa gcttatactc gttgcgaacg ttgcggacgt ccacattcag tttatcgtaa
    37561 gttccatctt tgccgtattt gcttccgcga acttgcctat aaaggtcaaa ttcccggcgt
    37621 gaagaaagct agctggtaaa aagtaaattc gcataagaag gaggtaaaag atcaatggtc
    37681 atgacagatc caattgcaga ttttctaact cgtatccgta acgcaaacat ggttaaacat
    37741 gagtctttag aagtacctgc atcaaaaatt aaacgtgaca tcgctgaaat cttgaaacgt
    37801 gaaggtttcg ttcgtgatgt tgaatacatc gaagatgaca aacaaggcgt gatccgtgtt
    37861 ttcctaaaat ttggtaaaaa cggcgaacgt gttatcacaa acttaaaacg tatctctaaa
    37921 ccaggtttgc gtgcttatgt caaagctgac gaagtaccaa aagtattgaa cggtttaggt
    37981 atcgcaatca tttcgacttc tgaaggtgtt gtcactgata aagaagctcg tgaaaaaaat
    38041 atcggcggcg aagttatcgc ttacgtatgg taatcaagta gaatctacaa ggaggtgtct
    38101 ttaagtgagc cgtatcggaa ataaagtcgt tgttatccct gaaggagtaa cagttactca
    38161 agacggaaac aacgttacag ttaaaggacc aaaaggtgaa ttgactcgta ctttttcagc
    38221 agatatcaaa atgaatatcg aaggaaacga agtaactttc actcgtccaa acgacagtaa
    38281 agaaatgaaa acaattcatg gaacaactcg tgcgaacttc aacaacatgg ttgtaggtgt
    38341 cagcgaaggt ttccaaaaag cgttagaact aatcggggtt gggtaccgtg cacaattaca
    38401 aggatcaaaa cttgtattga acgttggtta ctcacatcca gtagaaatca caccaccagc
    38461 tggagtaact gttgaagtac cagctaacac acaagtaatt gttaaaggtg cgaacaaaga
    38521 agaagttggc gaattagctg caaacattcg tggaactcgt cctccagaac cttataaagg
    38581 taaaggtatt cgttatgttg gcgaatatgt acgccgtaaa gaaggtaaaa ctggtaaata
    38641 atcgtttaga aaaattgcat agaagccagc agcaggaaaa aggcatttgg ctgaagttta
    38701 ccttcaaaaa aatacatttt tacttgtgcc gcagcttcta tttttcgaaa cgtatttact
    38761 gcttgaaata gtagtacaat aaaaataaag aggtgacaat tgtgattaca aaaccagata
    38821 agaacaaaac acgtcaaaag agacatcgtc gtgtacgtaa caatatctct ggtactgctg
    38881 agcgcccacg cttgaacgtt tttcgttcta acaaaaacat ctacgctcaa attattgatg
    38941 acgtagcggg tgtaacgcta gcaagtgcct ctaccttgga taaagaaatt tcaggtggaa
    39001 caaaaacaga agcagcacaa gctgtcggta aactagttgc tgaacgtgca gctgaaaaag
    39061 gcattaaagt agtagtcttt gaccgtggtg gataccttta ccatggccgt gtgcaagctt
    39121 tagctgaagc tgctcgcgaa aatggactag aattttagaa gaaggaggaa taccattcat
    39181 ggtttatatc gatccaaaac atttggaatt agaagaccgc gttgttgcaa ttaaccgtgt
    39241 aacaaaagtt gttaaaggtg gacgtcgtct acgttttgct gctttagttg ttgtcggtga
    39301 caaaaacgga cacgtaggat ttggtacagg taaagctcaa gaagttcctg aagcaatccg
    39361 taaagcaatt gaagatgcga agaaaaactt agttgaagta cctatggttg gttcaacaat
    39421 cccacacgaa gtaatcggtg cttttagtgg tggtagaatc ctaatgaaac ctgctgtaga
    39481 aggttctggg gttgctgctg gtggaccagt tcgtgccgta ttagaattag ctggggtagc
    39541 agatatcact tctaaatctt taggctcaaa tacaccaatc aacgttgttc gcgcaacagt
    39601 tgaaggtttg aaacaattga aacgtgcaga agaagttgca gaacttcgcg gcaaatcagt
    39661 tgaagaatta atcggataag gaggacaaca ataatggctg aattaaaagt tactttaaaa
    39721 cgcagtatta tcggacgtcc tcaaaaccaa cgcgatacag ttaaagcgtt aggtcttaca
    39781 aagattaaca gttcagtggt taagccttct aatgaagcaa ttaaaggcat gatcaatact
    39841 gtttctcacc tagtggacgt tgaagaaatt taatttagaa aaatcataac tagaacatta
    39901 aggaggtgcc aaacctatga aacttcatga attaaaacct gctgaaggtt cacgccaagt
    39961 acgtaaccgt gttggtcgtg gtacttcatc tggtaacgga aaaactgccg gacgcggaca
    40021 aaaaggacaa aaagctcgtt caggcggcgg tgtgcgttta ggatttgaag ggggacaaac
    40081 accattgttc cgtcgtttac caaaacgagg attcacaaac gtgaaccgta aagactatgc
    40141 agtcattaat cttgatgtat tgaaccgctt tgaagacgga gctgaagtaa ctcctgttgc
    40201 tttagttgaa gctggaatcg tgaaaaacga aaaagctgga atcaaagtat tagctaatgg
    40261 ggaactaaac aagaaattaa ctgtgaaagc agcaaaattc tctaaagcag ctcaagaagc
    40321 aattgaagcg gctggtggat caatcgaggt gatctaatgt ttagactatt aaaagatgct
    40381 tttaaagtaa aagacattag atcgaaaatc ctatttactg tatttgtgtt gtttgtattt
    40441 cgcctaggtg cacatattac tgtaccaggc gtaaatgctg cttcattgca aaacctttct
    40501 tctttgccat ttttcaacat gttgaacttg gtaagtggta gtgcgatgca aaacttttcc
    40561 attttctcaa tgggggtttc accatacatt actgcttcta tcgtcattca gcttcttcaa
    40621 atggacatcg tgcctcgatt tgttgaatgg tcgaaacaag gggaagttgg tcgtaaaaaa
    40681 ctgaatcaag cgactcgata tctgacatta gtattggcat ttatccaatc tgtcggtatc
    40741 actgctggat tcaatcaata cgctcaatta ggtttcgtga ataatcctaa cgtagcaact
    40801 tacgtaacga tcggtttgat tctaacagcc ggtacgatgt ttgttacttg gctaggagaa
    40861 cagatcacag aaaaaggtat cggaaatggc gtatcaatga tcatttttgc aggtatcatt
    40921 tctagattgc ctgaaagtat cagagaaatt tatgaagatt actttataaa tattgatcct
    40981 tcagacatat ggaaatctgc tatttttgtg gccattttac tagtagcggt cttgttgatc
    41041 gtgacattcg taacattttt ccaacaagct gaacgcaaga tcccgatcca atatacgaaa
    41101 cgcgtagctg gcgcgcctac aagcagctat ctgccgttga aagtaaatgc agctggagtt
    41161 attccagtta tctttgctag ttcgtttatt gctacgccaa atgctgtttt acaagcattg
    41221 agcggtagat tcggttctga aaactggtac aaagtgatga tggagatctt taactataat
    41281 acagcacctg gtgctacgat ttatacgatt ctgatcgttg ccttcacttt cttctacgcc
    41341 tttgttcaag taaaccctga gaaattagcg gaaaacttgc aaaagcaagg aagttatata
    41401 ccaagcgtac gccctggtaa ggggactgaa gagtatgtct caagattact gatgcgttta
    41461 agtacagttg gtgcagtctt ccttggttta gtggcattgc ttccaatcat tgctcaaatg
    41521 gtttgggatc ttccacaatc aatcggatta ggcggtacaa gcttactgat cgttatcggt
    41581 gtagctttag aaactgcgaa acagttagaa ggcttaatgc tgaaacgtaa atatgttgga
    41641 ttcattgagt aagaaagcgt gttgaggttt cccctcaaca cacgttctca cacatttatt
    41701 aggagggaaa acaatgaacc tcattttaat ggggctgcca ggcgcaggta aaggaacaca
    41761 agctgaaaaa atcattgatg cttatggtat tccgcacatc tcaacaggag atatgttccg
    41821 cgcagcgatg caaaacgaaa cagctcttgg cctggaagca aagtcttata tggataaagg
    41881 tgcattagtt cctgatgaag taacaaatgg aatcgtgaaa gagcgactag ctgaaccaga
    41941 tacagagaaa ggcttcttat tagatggctt tccacgtacg ctagatcaag caaaagctct
    42001 ggacgcaatg ctaaaagatt tgaataaaaa aattgatgct gtcatcgata tccatgttgg
    42061 tgaagaaatc ttggtcgaac gtctagccgg ccgatttatc tgcagcaatt gtggagcaac
    42121 ttaccataaa attttcaacc ctacaaaagt tgaagacaca tgtgatcgtt gcggtgggca
    42181 tgaattctat caaagagaag atgataaacc tgagacggtt aaaaatcgtc tggctatcaa
    42241 catcaaaaat agtgaaccaa tcttggctta ttataaagaa caaggtttgc taaacactat
    42301 tgatggtgct cgtgaaatcg atgctgtatt tgcagatgta caaaaaatca ttgaaaaata
    42361 gcacgattgc ccgtagccga cttgcattat gcagttactc ttgtcaagtt caataaatta
    42421 tgatagaata tgtcagtccg acttctagag aatcctctag atgatccatt ttgagaattt
    42481 tgaggtggga actgcagttt ccgccatttt atcgtgtgaa aatgcgaaac aagtacaagg
    42541 aggtactgtg cgtggcaaaa gaagatatga ttgaagtcga aggtacagtc gtcgaaactt
    42601 tgccgaatgc aatgtttaaa gttgaactag aaaacggaca ccaagttctt gctactgttt
    42661 caggtaaaat tcgtatgcat tacatccgta tcttacctgg tgacaaagta acggttgaat
    42721 tgtctccata cgatcttaac cgtggccgta ttacttatcg ctttaaataa ttgtactccg
    42781 taaaactaca ggaggtatta tcatgaaagt aagaccatca gtaaaaccaa tgtgcgaaca
    42841 ttgtaaagta attcgtcgta aaggacgcgt tatggtgatt tgcccagcaa atccaaaaca
    42901 taaacaacgt caaggataat tggaggtgct ataaatatgg ctcgtattgc aggagtagat
    42961 attcctcgtg ataaacgtgt agtagtttca cttacttata tctatggtat tggtaacact
    43021 actgctaaaa agatcttagc tgatgctggt gtatctgaag atgttcgcgt tcgtgaatta
    43081 acgaatgaac aaacagatgc tatccgtgca gaagtagata aattaaaagt tgaaggggat
    43141 cttcgtcgtg aagtgaactt gaacatcaaa cgtttgatgg aaatcggttc ttaccgtgga
    43201 atccgtcatc gccgtggatt gcctgttcgt ggacaaaaca cgaaaaacaa tgcacgtact
    43261 cgtaaaggcc cagctcgcgc tatcgctggc aagaaaaaat aattcttaag tgaaggaggt
    43321 taaaacttca tggcagcaaa aaaagtgaat cgtaaacgcc gtgtgaagaa gaatatagaa
    43381 gcaggtattg cacatatcca ttctacattc aataacacta tcatcatgat cactgatact
    43441 cacggaaatg cattagcgtg gtcatcagct ggttcattag gtttcaaagg aagcaaaaaa
    43501 tcaactcctt ttgccgctca aatggcagca gaaactgcta caaaagcagc aatggaacac
    43561 ggattaaaga ctgttgaagt aacagttaaa ggacctggtt ctggacgtga agcagcaatt
    43621 cgttcattac aagctacagg tttagaagtg actgcaattc gtgacgtgac tccagttcct
    43681 cataatggtg cccgccctcc aaaacgccgt cgtgtttaat gagtgccgct cattttgagt
    43741 tttatcagta acgtcattga acaagacact tttcgttttg aaaggggtaa agataagaat
    43801 gattgagttc gaaaaaccaa gaatcgcaaa aattgatgaa gaaaaagatt atggcaagtt
    43861 catcgttgaa cctcttgaaa gagggtatgg gactacttta gggaattccc tacgtcgtat
    43921 tttattatct tctttgccag gtgctgccat cactaatatt caaatcgatg gtgtactgca
    43981 tgaattctct accatcaaag gtgttagaga agacgtcact caaatcatct tgaacattaa
    44041 aggcttagcg ctgaaaatgt atggccaaga agaaaaaacc cttgaaatcg atattaccgg
    44101 acctgctaca gtaactgctg gcgatattat cgttgatagt gatgtagaaa ttcttaacaa
    44161 agatatgtac atctgtagtg tctctgaagg agctacgttc catgctcgct taacagtgaa
    44221 acctggacgt ggttatgttc aagcagatga aaataaaaaa gaagatatgc caattggcgt
    44281 tcttccagtc gattcaatct acacacctgt tcgtcgtgtg aactatcaag ttgagaacac
    44341 acgtgttgga caccgtgaag attttgataa attgacgatg gagatttgga cagatggttc
    44401 aattgaaccg ttggaagcaa tgagtttgtc tgcgaaaatc atgactgaac atttggatat
    44461 ttttgtgaac ttaactgatg aagcaaaaaa tgctgaaatc atggtcgaaa aagaagaaac
    44521 acaaaaagaa aaaatgttgg aaatgacgat cgaagagtta gacttgtctg ttcgctctta
    44581 caattgtttg aaacgtgcag gaatcaatac tgtacaagaa cttacaaata aatctgagcc
    44641 tgaaatgatc aaagtccgta acttaggacg taaatcatta gaagaagtta aagcaaaatt
    44701 gcacgactta ggtttaggtt tacgtaaaga agattaaaac cttttacgaa ggagggaaac
    44761 caacgtgagt tatcgtaaac taggacgcac atctagccaa cgtaaagcga tgttgcgtga
    44821 tttaacaact gatttattaa tcaacgaacg tattgtgact actgaagctc gtgcgaaaga
    44881 agttcgctca actgcagaaa aaatgatcac tttaggtaaa cgcggagatt tgcatgcacg
    44941 tcgtcaagct gcggctttcg tacgtaatga agttgccagc gtgcgtgaag aaaatgagga
    45001 catcgtcatt gaatcagctt tacaaaaact gttcaacgat attgcacctc gttacgcaga
    45061 acgtcaaggc ggatacacac gcattttgaa gacagaacca agacgcggtg atggtgcacc
    45121 aatggttgtc ttagaacttg tctaaaatca cactaataac atcactttat agatgattct
    45181 tttagagtgt tatgatgttg gaagatattt ttccgagtct agctcagcgc tgctcctcaa
    45241 gcgaatatcg cttggggagt aagcactcat cataaagtgt ttttttatac cttcaattac
    45301 ctgtcatgac agatatgttt gaaggcataa aaaaacattt taagcgagaa ataattgaaa
    45361 attttgtctt tttttgatag tgttagtagg aactttagga tgaagaagga gtaatcatgg
    45421 agccaattat tgaattagaa aagataaatt ataaatacca gccagatgat cttcgccccg
    45481 cattgaaaga tgtctccttt acaattgata aaggtgagtg gatcgctatt attggacaca
    45541 atggttcagg aaaatcgaca cttgccaaaa cgatcaatgg attgcttctg ccagaatcag
    45601 ggatcgtgaa agtgggaaat caaatattgg atgaagaaaa tatttggacg atccgacaga
    45661 tggttggaat ggtttttcaa aatccagaca atcagtttgt tggttcgaca gtagaagatg
    45721 acgttgcttt tggtcttgaa aatcaaggga ttcccagaga agaaatgcta gtgcgtgtga
    45781 aagatgcact tgagaaagtt agaatggctg agtttgcttc acgcgagcct gctcgtttat
    45841 caggagggca aaaacaacgt gttgccattg ccggcgtggt tgccttacga cctgatatca
    45901 ttattttaga tgaagcaacc agtatgctag atccagaagg tcgtgaagaa gtgatctcta
    45961 cgatcaagaa gatcaaagaa gaaagtcaat tgaccgttat atctatcact catgatattg
    46021 atgaagcagc taatgccaat cggatactag tgatgagaca aggcgaactt gtccgtgaag
    46081 gtacaccaaa agaaattttt tctgctggtc ctgaactgat cgacttaggt ctagatctac
    46141 catttcctga aaaactgaag agcgcactga aagaacgtgg ggttgatgtg cccagtgaat
    46201 atatgacaga agaaaggatg gtggattggt tatggacatc cgttttgaac aagtagactt
    46261 cacttatcag ccgaatacac catttgaaca aagagcctta tttgacatca atatgacgat
    46321 caaagaaaac agttatactg cattagtagg gcataccgga agcgggaaat caaccttact
    46381 tcagcatttg aatgcgcttg ttaagccaac aagcggtaca gtccatattg gagaacgaga
    46441 tattcagccg gatacggata ataagaattt aaagcccatc cgaaaaaaag ttggcatcgt
    46501 ctttcagttt ccagaagcac agctattcga agaaacggta gcaaaagaca ttgctttcgg
    46561 tcctaaaaac tttggtgtca gcgaagaaga agcactagtc ctagcaaaag aaacattaga
    46621 acaagttggg ctggatgaaa gctatttgga acgttcgcca tttgaacttt caggagggca
    46681 aatgcgtaga gtagcaatcg ctggtgtgct tgccatgagg ccggaagtac ttgtattgga
    46741 tgaaccaacg gcaggacttg atccccaggg cagaaaagaa atgatggaga tgttttggcg
    46801 tcttcataag gaacagcaga tcactattgt attagttacg catttaatgg atgatgtagc
    46861 caattttgca gattatgtct atgtgttgga aaagggacga atcgttaata gcggggagcc
    46921 gcaagaggtc tttcaaaaca ttgaatggct aaaagaaaaa caactaggcg tacctactgc
    46981 caccgagttt gcagaagaat taatggctaa agggatgaac tttgctacct tgcctttgac
    47041 agctgaagaa ttagcagacg ctatcgtcca ttatgctgga ggaaatgccc atgatgaata
    47101 aattgatatt tggtcgttat atgcctggag attccttgat ccatcgattg gatccccgag
    47161 ctaaattatt agccagtttt tattttatcg gaatcatttt tttagcaaat aattgggaaa
    47221 cgtacctttt gttggcattt ttcacattac tttctgtctt cttgtcaaaa gtagatttga
    47281 aattttttat ccgaggcatt cgcccattga tctggctaat cctcttcact gtagctttgc
    47341 aggtcttgtt tacaagtggc ggagaaactt attggagttg gggtgttttc catatcactg
    47401 aatttggtgt gatgaacggc ctctttattt tctgtcgatt cgtgttgatc atttttatgt
    47461 cgactctact cacgcttact acaccgcctt tggaattatc ggatgcgatt gagtacttgc
    47521 ttcgtccatt gaaagccgta cggttcccag tccacgaaat ttcattgatg ctgtcgattg
    47581 ctcttcgttt tgtgcctaca ttgatggacg aaacagaaaa gatcatgaac gctcagcgcg
    47641 ctcgcggtgt tgattttggt gaaggaaacc tagttcagaa aatgaaagca gtcattcctt
    47701 tgctgattcc tttatttgtc agcagcttca accgtgcgga agatcttgcg actgctatgg
    47761 aagcaagagg ctacaaaggt ggggaaggaa gaacgaaata tcgtgtattg cattggcata
    47821 atagagacac gatggttatc attgcttttg tattattaac tgtgctactg atctttttaa
    47881 gaggataaac gttcagtggg ccgctatcta ttcttttctt gctgatctgt ttgcaagaaa
    47941 ggatgcaagc agtgtatgac aagaaatatt agggtctgtg gcagactaag gtcacaggcc
    48001 tctttctttt atctgaaaag aaagaacgaa aaa
//
LOCUS       DAZKLM010000009        47563 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3764
            SAMEA115706913-rid22582563.denovo.009, whole genome shotgun
            sequence.
ACCESSION   DAZKLM010000009 DAZKLM010000000
VERSION     DAZKLM010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706913
            Sequence Read Archive: ERR13196647
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 47563)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47563)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287823.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287823.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,004
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,849
            CDSs (with protein)               :: 2,849
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 41 of 96
            Pseudo Genes (incomplete)         :: 52 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 15 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47563
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706913-rid22582563.denovo.009"
                     /isolate="VRE3764"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            137..1018
                     /locus_tag="ACG842_000468"
     CDS             137..1018
                     /locus_tag="ACG842_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFQ0247033.1"
                     /translation="MAEIVKVTHLKKSFGRFEALKDVSFTVHSGEVVGFIGPNGSGKS
                     TTIRTLLGIIKKDGGTATIFGQDVWEDSLSIHERLSYVPGDISLWGNLTGGAIIDLFM
                     KLHGKGSAQKKDELIQRFSLDPKKKAKNYSKGNRQKVGLIAALAVDSDLYLFDEPTSG
                     LDPLMETIFQEEVEKLKEQGKSILLSSHILSEVERLADKVVIIREGTLDEEGTLDELR
                     HLTRSTITIETEAENSHLAQLNGVHDFVQKGHLAQFAADNEAFDVILAEASKLGVKKF
                     ESIPPTLEDLFMRHYEG"
     gene            1031..2641
                     /locus_tag="ACG842_000469"
     CDS             1031..2641
                     /locus_tag="ACG842_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0247034.1"
                     /translation="MTDKFSRWSTLFSQNLSRDWKKILIWLVDVTAFSGGFVPAFEEL
                     TSGQGLAGLYETLQNPAMIAMVGPTPASNAAEYTLGAMYANEMLLFCSLFAAVISVLH
                     VVSQTRKKEELGLIELIRSFRVGRQANSLAVIAESILINLLLAISISCTMIFFQADSI
                     TAEGAWLFGLSVGSMGILGSAVALFAAQLMPSSASSNGLSLAFLGGLYLLRAITDVAD
                     SSLSMWNPLGWSYLTYPFTTNNWIPLLYLFLFSLILIISSFVLEEHRDLNTGYIPERE
                     GKPYAKRSLLSLRGLLFYLNKGMTISWLITFGLMGAAYGSIYGNMQQFLESNDLMKQM
                     FIQSGVSIEKNFTSTIMVVLICLAAILPIASINRLFTEETHGRMNQLFATKISRNQLY
                     WTNMLLAILFAILAILISSGALGLSALASMKDAKDMDLLMFLKAGFNFLPAVLVFIGI
                     SGFLFGWLPRIGKLAYIYLAYSFALNYFGGILDLPEWFSKTAVPSWVPRMPNDSFDLS
                     VFLTLSCISLALFVLGSIGYRRRDLLEG"
     gene            complement(2718..3377)
                     /locus_tag="ACG842_000470"
     CDS             complement(2718..3377)
                     /locus_tag="ACG842_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HFQ0247035.1"
                     /translation="MSHDHLCVSFVPLFNHLVQQDQEKVHALVKHHYVEKGEQILAPN
                     GEPQLVIVASGSMKVYQLSASGKEQLLRVIEPGGYEGESQLLGVPNESLFGEALEKTE
                     ICILRQKDFSELLLHYPALSLKLLAINVEKLTKVQQQTTFLMMEKVEERVAVYLLDLA
                     KATGKDHFFLPMKMKELAAFIGTTPETLSRKFKLLEENGYVKRKNREVWIIDKEQLED
                     L"
     gene            complement(3660..4325)
                     /locus_tag="ACG842_000471"
     CDS             complement(3660..4325)
                     /locus_tag="ACG842_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate 1-dehydrogenase family
                     protein"
                     /protein_id="HFQ0247036.1"
                     /translation="MELGKSDYQLFDRPIYAFKQLKESHPTDKIEQIKKEYKEHWQKW
                     KEIQLQTAALLPDMYGMSKPKIESWTNGWNLRSHFWSAYRSESRQDENACLAVLLNQK
                     QYQIYLMYQHYKSEERYGSIAAYNQLLAILKEWSKTVDIKDYYIWPQPEHELDDHLAL
                     SDYLSDTKKQEELKKAMRDRTFQMGKLFFYPQEIEHVEQITAETLQELAPLYQKLGAE
                     KFQ"
     gene            complement(4472..5077)
                     /gene="dhaL"
                     /locus_tag="ACG842_000472"
     CDS             complement(4472..5077)
                     /gene="dhaL"
                     /locus_tag="ACG842_000472"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaL"
                     /protein_id="HFQ0247037.1"
                     /translation="MKLTVQEIQEWLTQFAEAINQNKQYLSDLDTPIGDGDHGNNMGR
                     GVSAYEEAFQADHPETISDTFKVFSMAMISKVGGASGPLYGSAFMNMMKATKEVDSID
                     SQEKLGEVIEQGTAGIQSRGKAEADDKTMLDVWLPVTEALKAGNLTKEIIDQAKEHTK
                     VLVAKKGRASYLGERAIGHIDPGAASSAILFSTLLDVINQP"
     gene            complement(5105..6115)
                     /gene="dhaK"
                     /locus_tag="ACG842_000473"
     CDS             complement(5105..6115)
                     /gene="dhaK"
                     /locus_tag="ACG842_000473"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004371 - glycerone kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HFQ0247038.1"
                     /translation="MPERKDGNKLKKIMNDPSNIVEEMLEGLVKSYPELVHRVESSRV
                     VAKNQKAEQVGLVSGGGSGHEPSHAGFVGEGMLSAAVLGDVFTSPTPDQIQTAIKEAD
                     SGKGVLLIVKNYTGDALNFDMAKELAAMDDIEVESVIVDDDIAVENSTYTAGKRGVAG
                     TVLVHKIVGDAARNGASLAELKELGEKVVQATKTIGLALRAATVPEVGKPGFELGEDE
                     IEYGVGIHGEPGYRREKMQPSKVLAKELVTKILDDYSNLPKEAGVLVNGMGGTPLMEQ
                     FVFMNDVLALLEERGVNVVFRKVGNFMTSLDMQGLSLTLIDLTETQWKDSLESNVQTI
                     SW"
     gene            complement(6090..6485)
                     /gene="dhaM"
                     /locus_tag="ACG842_000474"
     CDS             complement(6090..6485)
                     /gene="dhaM"
                     /locus_tag="ACG842_000474"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase phosphoryl donor subunit
                     DhaM"
                     /protein_id="HFQ0247039.1"
                     /translation="MKKSILLISHSQKVTDGIKEMIEQMQKSEEVQIFSLGGINGEIG
                     SDPMKIVEAVNESSDAASYFVFADIGSAVLNSELAKDMLEEEQQQRYHLVDAPLVEGA
                     FAAAITAGITDDTQQILTEAQNAGKKGWE"
     gene            6639..6959
                     /locus_tag="ACG842_000475"
     CDS             6639..6959
                     /locus_tag="ACG842_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HFQ0247040.1"
                     /translation="MITTTTNSVEGKAVVAYKGIVFGEVITGIHAFKDLEAGLRNIFG
                     GRSKSYENELMGAREEALAEMAERAKALGADAIIGVKMDYEVLGSDNGMLMVTCSGTA
                     VSTR"
     gene            complement(7071..7928)
                     /locus_tag="ACG842_000476"
     CDS             complement(7071..7928)
                     /locus_tag="ACG842_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HFQ0247041.1"
                     /translation="MKKRMKPLIWPIAVSSIFLLGQTAFAETSEPAQTEPSTTISSSG
                     AENGASETEMTSNVPTDNSGETISSSEDTSINSSTSSDQIDHLQDLENKRKELEEALE
                     AAKNKLKEDQAAIDQDLADLKNKVGNDRAQLEKKLAEAKAKAEAVQTDLDGKIQQAKE
                     FNQQLNQVAQTASGKLSQTQADITAASQAIHGKISEAQTVANDFEAKKADLAASFQRI
                     KDILHSAGEKSVTSGSTTETVQQTTEPSKSTSQTLPKTNEKTASVSYSLAGAGVLVAA
                     AYGIIRKKK"
     gene            complement(8036..9340)
                     /locus_tag="ACG842_000477"
     CDS             complement(8036..9340)
                     /locus_tag="ACG842_000477"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFQ0247042.1"
                     /translation="MISITHTGNLFLDTCLSIMYFFLVSYPILGGFVWFIGVWCYVFL
                     YKHKQKEWVDVPLSVEPFITIMVPAHNEEIVIEYTIEYLMTKLNYHNYEVLVTDDGST
                     DQTPEILARLMKKYANLRVVRIEKNKGKAHAFNIGLAFAKGKLILSNDADTVPEPDAL
                     IRYVNYFIRPGARHIAAVTANMDVQNRTKLIAKSQTVEFSSIVGIIKRTQSAVFGGLY
                     AYSGANTMYRKEALIDVGGFRQDRATEDISIAWDHQLNDWLSVFAPEIIFFMEVPVTL
                     KMLYRHRKRWAKGGTEVWLTNFKKVMLHPFKHIGRTIIFIDQTLSIVWSIFFCISVVL
                     FAGLIGHYVYQGNYEQIYITFTFSFVFICFEMVAGFFQLLASLIVDDRRRKLKYLLFA
                     PLYMLLFWIVNAITIVTTFIPAVKTILGYGSGTWKSPERTKK"
     gene            complement(9337..9846)
                     /locus_tag="ACG842_000478"
     CDS             complement(9337..9846)
                     /locus_tag="ACG842_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0247043.1"
                     /translation="MKKVTREEVASILVKDKYFSKGNYWLKIWQTLVALLGWMIVVLP
                     FIWVFFPLTRPERAKDFSLYAFLSEKKMLYFLIGFFVLIFFSFLITSFVLTRWNNRNL
                     YKKVNKSFEYEDEKLKKRQELLEEVYTERFGTKEERETVRFYSVSEEKNLDTTFIADL
                     YKENGVELK"
     gene            complement(10021..12156)
                     /locus_tag="ACG842_000479"
     CDS             complement(10021..12156)
                     /locus_tag="ACG842_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HFQ0247044.1"
                     /translation="MVNTQKQLEEAYLKNVYQKLIDKKNELRESMDTAKIEGNQSLKE
                     MAGELRLNFDSYLDNLDTFSMIEMKNREIDQMNIKQKNAENQLAKIERLLKSPYFGKV
                     VVDFLDNEPKESFYIGTANFTDDADENLVYDWRSPIAELFYNNMIGPSSYTVRQNKID
                     TVIKERRQFLLERDRLVKFFDTSVAIQDDVLLEVLGQDETNEMKAITATIQSEQNTII
                     RDVKSQNILVNGVAGSGKTSAIMQRIAYLLYLYRDTMTSDDLLILSPNHRFIDYISNV
                     LPSLGERNPLNLTIIQLVSQLSAEEIEGEEAYFKRITGENVSEQTERLRSKKFIDNLK
                     QSDPLFLDHPNFIRGLTKNGKTVLSKKTIEKIYEKVPAHPKLIDRLQATKKALMSEWK
                     NHLLKQAKSPAVQNQVLSLTEDRQLELFGKLISDDSEQSIVAYARKLLQKKYRKITRQ
                     IEEMAWVDEHQLFERIYEKRYGSAYAWQPTRTVDEAVIILAIRHLLVEKVNVPAFRYL
                     LIDEVQDYTLAQLGLLIELFPKTHFTLVGDENQAIFNSSTTFADIMRCFDDYHLPIHR
                     YDLRNSYRSSGAITELFKTYAVDQEKIDIVSIRPQGKEPEYRSFRSSEELLKILAEIL
                     VSLKGEKAAVITQTEEETQTLQESIKKNSYGLENCQIIPLSLAKGLEFDHVILYPFEN
                     DGDEQRRRRQMYTAISRGMKSIVVLERAT"
     gene            complement(12239..13894)
                     /locus_tag="ACG842_000480"
     CDS             complement(12239..13894)
                     /locus_tag="ACG842_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFQ0247045.1"
                     /translation="MKIVIVGGVAGGMSAATRLRRLMEDAEIVVFEKGPYVSFANCGL
                     PYYLSGEISERENLLVQTPESLSARFCLDVRPNHEVTAIFPENKTVEVVHEGQKHIEQ
                     YDALVLSPGAKPVVPSIPGITEADNVFSIRNVPDIDKVMHALEKQPKRAVIVGAGFIG
                     LEMAENLKRRGLEVMVIEQAPHILPTLDEEMAAFIEKELSHQGVEVITSHAVAGFEDH
                     GKRLRLDDGRTIPADLVILSIGVRPDNQLAVTAGIELGIRGGILVDERYQTNIPDIYA
                     VGDAIVVKQQITGKDALISLASPANRQGRQVADTISGISRRNQGGIGTAIIRTFGMTA
                     ASTGLSERTAKENELSFEVIHVSGKDHASYYPEATDILLKLIFHPETGEIYGAQGVGA
                     KGVDKRIDILATAIKGHLTIFDLPELELTYAPPFGSAKDPVNMLGYAAMNIAEGLSET
                     VQWHELPTELAKGKILLDVRTAEELEKGKFKEAKHIPLNELRDRLNELDSQQEYIVSC
                     HSGLRSYLAERILKQSGYHVKNLDGAFSLYQTVRPEELIYPNK"
     gene            complement(13896..14210)
                     /locus_tag="ACG842_000481"
     CDS             complement(13896..14210)
                     /locus_tag="ACG842_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFQ0247046.1"
                     /translation="MNLLFQSIKSITVPELKEKLLENPALLDVRTPAEYRGGHIKGAK
                     NVPLQSINRYDGDKEKTVYVICQSGMRSKQAAKELKKSGYDVVNVRGGMNQWFDRTVG
                     GK"
     gene            14380..14640
                     /locus_tag="ACG842_000482"
     CDS             14380..14640
                     /locus_tag="ACG842_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HFQ0247047.1"
                     /translation="MEIDNKNILNRLKRTEGQIRGIQKMIEDEKECMDVITQLSAVRS
                     SIDRVMGMIVADNLKNCFEKPDSNPEEQAAKLEQAIKMIIKK"
     gene            14654..14968
                     /locus_tag="ACG842_000483"
     CDS             14654..14968
                     /locus_tag="ACG842_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HFQ0247048.1"
                     /translation="MKVVFHIDELEKWSETGKNVKNLIKASPETTIVVSVNGIAITGY
                     LDSANAEFLDLQGVVFHACANAMRANHISESSLPEQVIVVPAGVLDLVELQSQGYAYI
                     KP"
     gene            complement(15698..16252)
                     /locus_tag="ACG842_000484"
     CDS             complement(15698..16252)
                     /locus_tag="ACG842_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HFQ0247049.1"
                     /translation="MKLSTDWRKEIQTIPNLLSIFRIFLLPIYLYFVLRQSFYIAGAV
                     IVVSGLSDYLDGVIARRYNQVTDLGKVLDPFADKLTQLFLILSMAWYRPWLWLLFGLF
                     LIKEGFMFVAGLIGLSKNIKLSGAKWYGKVATAVIYVGMILLLLFPELPTLWVRVIFA
                     VITYGLLQSFVLYAVEYRKMFQRK"
     gene            complement(16378..18307)
                     /locus_tag="ACG842_000485"
                     /pseudo
     CDS             complement(16378..18307)
                     /locus_tag="ACG842_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336853.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
     gene            complement(18613..19329)
                     /locus_tag="ACG842_000486"
     CDS             complement(18613..19329)
                     /locus_tag="ACG842_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MIP/aquaporin family protein"
                     /protein_id="HFQ0247050.1"
                     /translation="MNSDMTQIMGEFIGTLILVLLGDGVCAAVNLNKSKAQASGWIVI
                     AFGWGLAVTMAVYISGFMGPAHLNPAVSLAMAMTGAISWNLVVPFIIAQVLGAFAGAI
                     LVWLSYLPHWNATKDESAILGTFATGPAIRNYPANVITELIGTFVLVLGLLAFGQNEF
                     APGTNVFAVGGLILAIGLSLGGPTGYAINPARDFGPRLAHAVLPIANKGTSDWAYSWV
                     PIAGPMIGAIIAVGVYSLMV"
     gene            complement(19330..21153)
                     /gene="glpO"
                     /locus_tag="ACG842_000487"
     CDS             complement(19330..21153)
                     /gene="glpO"
                     /locus_tag="ACG842_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glycerol-3-phosphate oxidase"
                     /protein_id="HFQ0247051.1"
                     /translation="MFSNKTRQDSIQKMQQEELDLLIIGGGITGAGVAVQSAASGIKT
                     GLIEMQDFAEGTSSRSTKLVHGGIRYLKTFDVEVVADTVGERAVVQSIAPHIPKPDPM
                     LLPIYEGEGATTFNMFSVKVAMDLYDKLANVTGTKYENYTLTPEEVLEREPFLKKEGL
                     KGAGVYLDFRNNDARLVIDNIKKAAEDGAYLVSKMKAVGFLYDGDQIVGVKARDLLTD
                     EVIEIKAKLVINTSGPWVDKVRNLNFTRPVSPKMRPTKGIHLVVDAKKLPVPQPTYFD
                     TGKQDGRMVFAIPRENKTYFGTTDTDYQGDFTDPKVTQEDVDYLLDVINHRYPEANIT
                     LNDIEASWAGLRPLLIGNSGSDYNGGDNGSISDKSFNKVVDTVSEYKENKVSRAEVED
                     VLNHLENSRDEKAPSTISRGSSLEREPDGLLTLSGGKITDYRKMAEGALRLIRQLLKE
                     EYGIETKEIDSKKYQISGGNFDPTKLEETVTELAKEGVAAGLEEEDATYIADFYGTNA
                     RRIFELAKEMAPYPGLSLAESARLRYGLEEEMVLAPGDYLIRRTNHLLFERDQLDEIK
                     QPVIDAIAGYFEWTEEEKAQQTKRLEALIAESDLRELKGEK"
     gene            complement(21167..22663)
                     /gene="glpK"
                     /locus_tag="ACG842_000488"
     CDS             complement(21167..22663)
                     /gene="glpK"
                     /locus_tag="ACG842_000488"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HFQ0247052.1"
                     /translation="MTESTYIMAIDQGTTSSRAIIFDKKGRHIGSSQKEFTQYFPQES
                     WVEHDANEIWNSVQSVIAGAFIESGIKPNQIAGIGITNQRETTVIWEKDTGRPIYHAV
                     VWQSRQSAGIADNLKNEGYQEFFHEKTGLVIDAYFSATKIRWILDHVEGAQERAEKGE
                     LMFGTIDSWLVWKLTDGQAHVTDYSNASRTMLFNIHQLDWDQEILDLLNIPRVMLPKV
                     TSNSEVYGYTQGYHFYGSEAPISGMAGDQQAALFGQMAFEPGMVKNTYGTGSFIVMNT
                     GEKPQLSKNNLLTTIGYGINGKVYYALEGSIFVAGSSIQWLRDGLQMLQKASDSEDAA
                     KRSTSEDEVYVVPAFVGLGAPYWDQAARGAMFGLTRGTTKEDIIKATLQSIAYQVRDI
                     IDTMQDDTGIKIPVLKVDGGAANNEYLMQFQTDILNVPIQRAENLETTALGAAFLAGL
                     AVGYWKDTDEIREFYEAGKLFEVQMEEERREKLYNGWKKAVKATQAFE"
     gene            complement(22799..24259)
                     /locus_tag="ACG842_000489"
     CDS             complement(22799..24259)
                     /locus_tag="ACG842_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0247053.1"
                     /translation="MKIEALLERKEQLQILILRNLVLQGGTASINGLREHVQLSKASF
                     DQYLEDIELIGRMMEKKVEVQRNEYQALLVLDEDVSLEKILLFLLQESLKFKMLVYLL
                     EHQQVSIVRLATAFNISESSVFRKIKELNQLLEEFGLQIKNGQLYGEELQIRYFYYEL
                     FQYIPEDQRPLFLQNTPEKRPFILGLDRVLETTFTASAEAQIACWLGITKKRLLNEKS
                     TYATLKEKKLLYQSDRLYQAIDPIIVMHLSRTAAELNVYETMMFYSFFVSFSIVDEEI
                     FYQYDLTRSKKLPTAVLDTYIRETMLWHYRPRRLKIKEEKAVGYQISQINNEWFFFVG
                     KIEVYERDRLLEQQQKMLGHSLTQLLAKLQETAVQQLPRKRAEDSELSYLMIQYANVL
                     LMIDFYIAKPVVIGIDLESLPIYRIAFQQYLIRELRGIGGIEIGSYEEGKEYDLVITF
                     CQRNKKQCEYYLSEFASPYDIIRLKRRIETLKKEKN"
     gene            complement(24249..25544)
                     /locus_tag="ACG842_000490"
     CDS             complement(24249..25544)
                     /locus_tag="ACG842_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFQ0247054.1"
                     /translation="MNIVIVGGSFAGIHAAIYLRQLMPTSEIYLIEKQSKLGWIPSGF
                     NLILKGKVTSEKQLSWITKEELTDRYRIHVYTEKTVIGLKEKKVVLAGGKKLDFDRLI
                     LATGSNQKFRNISAESTLIHPVKKIQNVTALQQKIMNAKKIAIIGAGQAGIEIAEGLA
                     SRKKQIHLYESRKSILFRYLDPEMTEPLVKEMKNQGLSLFLEEQVVSLTENETAIVET
                     EKRKENYDLVLLANHSRPDNQMWEEQLTLNDDGTIWVDDYLQTSQKDVYAIGDAIQVT
                     FRPTNEKMYVSLVNNAVRTARNVSKTISGSQTKDQGTYRPVGNQWFGYFLGSVGLTEE
                     ESIFYPQKIETGYVDYKLTAINQSKVKLKFLFDSEKKILGAQLQSKKEMFHLLDQLTI
                     AVEEGWTFEQLEEHELFFQPEYRVPDMVWSKVANTAYED"
     gene            complement(25876..26553)
                     /locus_tag="ACG842_000491"
     CDS             complement(25876..26553)
                     /locus_tag="ACG842_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019172 - glyoxalase III activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HFQ0247055.1"
                     /translation="MKVLIVLTNVEKYEKIERPTGLWLSELTHFYDVLVKNGIDADFV
                     SPKGGYVPLEPQSVLNMDEIDWAYYEDEDFRNRALGNTLRPDDVDANDYAAVYYAGGH
                     GTMWDFPEAIDLGKVASQIYRNEGLVSAVCHGVVGLLPVTDESGKSILAGRTVTGFSN
                     EEEEANGTTNEVPFLAEDALKEKGANYQSGAAFSEVVQIDGRLLTGQNPQSAHQLGEA
                     VVEALKN"
     gene            complement(26583..27674)
                     /locus_tag="ACG842_000492"
     CDS             complement(26583..27674)
                     /locus_tag="ACG842_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HFQ0247056.1"
                     /translation="MSQFDQSITLPSGVQLKNRLMLSPMTTQLSFFNGVITEDEITYY
                     KQRSKGLGAVITGAANVQDIGKGWPGELSIAHDEMIPRLSELAKAIQGEGAKAIIQIF
                     HGGRMTSRATLSGEQPVSASAVAAKRPDAETPRAMSEEEIVETIQAFGKATRRAIESG
                     FDGIELHGANTYLIQQFFSPHSNRREDQWGGSLENRYRFIEELLTTVFSAVEQYAKKP
                     FIVGYRFSPEEYETPGIRFEDTIWLLERLRESKLDYLHVSLNMYDRIARSDKYNDKSI
                     LEVVHNTIQGKIPLVGVGGIRNREDVEQVLKYAELAAIGQQMIVDPDWAEKLLENRED
                     EFVTKPFEKAYKELYLPSPLYNFLKARYQ"
     gene            complement(27775..28209)
                     /locus_tag="ACG842_000493"
     CDS             complement(27775..28209)
                     /locus_tag="ACG842_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HFQ0247057.1"
                     /translation="MEKPIEKALDECLFFSVKKLDRMLNKLADEAFRRTGLAPTYGFI
                     LLILKEKDGIPQKDIAQMLYSAPSTIARFVEKLEYKGYVKTVSEGRLSLVYLTEQGRQ
                     FAKEVDGSWEELHQSYKAVLGNEASKELAQTMNDATDKLQKK"
     gene            complement(28793..30250)
                     /locus_tag="ACG842_000494"
     CDS             complement(28793..30250)
                     /locus_tag="ACG842_000494"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFQ0247058.1"
                     /translation="MKKFPEGFLWGGATAANQCEGAWDVDEKGLSVSDVYTFDSDLPK
                     EKWTDQWHMMTHQQVKAAQDPNSKYYYPKRHGNDFYHHFKEDIRLFAEMGFKCYRMSI
                     AWTRIFPHGDEETPNEAGLFFYDQVFDECLKYGIEPVVSLSHYEMPLYLVTEYGGWPN
                     RKLIQFYVRFAETVFKRYKNKVKYWMTFNEINCVKHHPFVSVGVIEENHPHLEQAKYQ
                     GAHHQFIASALATKACHEIIKGSQVGCMISYQLLVPYSCDPDDIQKTIEQQRTSLFFS
                     DVQARGYYPAYTQRMFEEKGVNLKIEVGDEEILAAYPVDFVSFSYYMSSAVSAHPENW
                     EGVQGNLITGGIKNPYLPSSEWGWQIDPKGLRIALNQLYDRYQKPLFIAENGLGAIDK
                     VEEDGTVQDDYRIDYLKQHIEQMKEAIKDGVDLFGYTSWGCIDIVSASTSQMSKRYGY
                     IYVDQDDEGNGTKKRLKKKSFDWYRQVIQSNGEIL"
     gene            complement(30247..32142)
                     /locus_tag="ACG842_000495"
     CDS             complement(30247..32142)
                     /locus_tag="ACG842_000495"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
                     /protein_id="HFQ0247059.1"
                     /translation="MEKPFSKNYTYLVRRIATALINKGEVKMDYSQLAKDIVRFVGGE
                     ENVSNVYHCATRLRFTLKDNKKADKEKVEQLEGVITVVEAGGMFQVVVGNAVNEVYDV
                     LSKQMKLEDDASSGKRGTEKKGILNSFIDMIAAVFAPTLGVLAGSGLIKGVLALCTSL
                     NLLTTESGTYIILNAAADAFFYFLPIFLAYTAAKKFNTDRFIAMVIAAALVYPTIVSA
                     YSDSITLRFLGMPVILARYTSTVIPAILAVWVLSYIEPKIRKSLHESIRNLLTPFICI
                     IVMVPIILLVVGPIADYASQLIAAGYLAVYNFSPVLSGAVIGGFWQVLVIFGLHWGLV
                     PVMTNNLSFYGRDTLGPACMTAVAAQAGAVLGVFLKTKNKKVKSLSLSAFITALFGIT
                     EPAVYGVTLKYKRPFYIACICGAIFGGVAGAAGAGALAVATRSILSFPIYIGEGFVWL
                     VASYFLAMISSCMLTFLFGYKDEIEEESSKDIVLSTPAAGEIIDLSEVNDPTFASGSL
                     GEGFAIIPTDGKIYSSVNGEVSTVFPTKHAIGVVSEEGAEILIHIGIDTVNLNGKYFQ
                     SAVSDGKKVRKGDLLMEVDLQELIKEGYDPTTMVIVTNSSRFKNIITSHKKNNQTRKL
                     ASLGGSI"
     gene            complement(32200..33975)
                     /locus_tag="ACG842_000496"
     CDS             complement(32200..33975)
                     /locus_tag="ACG842_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HFQ0247060.1"
                     /translation="MFITKRRCKQMDLNKRQLQILVVLRNKNHWITSEELAEALGTNK
                     KTIQTEIKNMLAIYEKKIIIQSNKRSGYFLESIESETKQQIIQEVTKHKIYSSMDFRA
                     STIVTYLLFQKGYVSMQKIADIFFLSKTAVSLQIKTILRWIERNPKLELEVSSTKGLK
                     IIAEENSKRIFLSLVGTETVIQHARFPQQISTIFSNIMPTVQKTLKEVLISYNYIVSG
                     EDFIRFSRYLVLTILRREELLEKTNRYTLFIPMVETLTKKLSSELPYSFSNQEKQAIE
                     NRLLELNYLMINDEKNKEIEKNLRAFEQTIIRFLDLPVSLLFHETEFLLTHIKQMKTR
                     MDAGHNTMNHFVQKIISRYPLEIYLIRRFFPIHFQLRPNLAESAYFVLYLAEALNPFR
                     KQGEILIVSNQPISILNTLKKQIQQSMNMWIKECRIEPVYLFKNQSNKITEYDAILTT
                     EQELIFEWDDLIYLPSVLDEFESWEIYQILREKLTIVEEKKKTKLLDHYYKEENQIKI
                     HKKIDSIQQLISPSSDLVMLPLNAETLLCCLIKSQVETKITEYQLEIPLIHQQRKIKR
                     VFFVSFDYKRNDILVFFQAVSDLLG"
     gene            complement(34025..35521)
                     /locus_tag="ACG842_000497"
     CDS             complement(34025..35521)
                     /locus_tag="ACG842_000497"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFQ0247061.1"
                     /translation="MDQEIFPENFLWGGAVAANQCEGAWLEDGKLPNVTDTLIGIMNQ
                     HPSIQWNEEKKIWEIALDESLHYLSHEAIDFYHRFEEDIRLLKELGLKAFRTSISWAR
                     IFPRGDEQKPNEAGLVFYDRLINTLNRYDIEPVITLSHYETPLALVGEYGGWQNRKLI
                     DFFEFYAQTVFERYQGKVKYWMTFNEINNAFRMPYAAAGLVTFPARDKLEPIATLTKK
                     VIYQACHHMFLANARATQLLKKIDPNAKMGIMCSFSALATYAYDCDPENVFGSLQFKR
                     NSWFFSDVMCRGHYPAYIYRTWKEEDCAPVMLDGDEQCLQANTADYIAFSYYRSAVYK
                     KEAEMRVDTGGANGFDNPFLKEKSPEPWSWPIDPLGFRYVMNELTDRYELPLFIVENG
                     IGLDEAPDELNRIHDPKRCEYLKIHLQEIAEAIKDGCQIIGYLWWGPIDIVSAGTGEM
                     RKRYGFIYVDRQNDGSGSFRRLKKDSFEYYKQIIQSNGKNLEYRSFFK"
     gene            complement(35715..37337)
                     /locus_tag="ACG842_000498"
     CDS             complement(35715..37337)
                     /locus_tag="ACG842_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HFQ0247062.1"
                     /translation="MEQKWWHHSVVYQIYPRSFQDSNQDGIGDLRGIISRLDYLAYLG
                     IDAIWLSPVYESPNDDNGYDISNYQAIMAEFGTMEEMEELIEEAKKRNIRIIMDLVVN
                     HTSDEHPWFIEARKNKENPYRDYYIWRDPVNGKEPNRLRSIFSGSAWQLDEKTGQYFL
                     HLYSKRQPDLNWKNKQVRQEIYQMMNFWIDKGIGGFRMDVIDMIGKEPDKEITSNGPK
                     LHEYLQEMNQATFGDKNLMTVGETWGATTEIAKLYSNPKRNELSMVFQFEHIGLDQQE
                     GKDKWDLKPLSISELKKVLSKWQTSLGDEGWNSLFWNNHDLPRIVSRWGNDDVYRKES
                     AKMFAILLHMMKGTPYIYQGEELGMTNCPIDDIAEAKDIETINMYNERISSGFTKEEI
                     LESIKAKGRDNARTPMQWNAREHAGFTTGIPWLRVNPNYKEINAEEALADQDSVFYMY
                     KKLIDLRRKHDIIVWGDYELVEKTPEHVFAYSRIYEDQQWLVICNMSSEKQTFTIPRE
                     ALRVIVSNYPLKQLPDGKVDLRPYEAFVLEVK"
     gene            complement(37665..38978)
                     /locus_tag="ACG842_000499"
     CDS             complement(37665..38978)
                     /locus_tag="ACG842_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HFQ0247063.1"
                     /translation="MKEKILSYFEIEKLNTTVKREVLAGFTTFISMAYILFVNPTVLG
                     ASGMDEGAVFTATALASAIGCVLMGVLARYPIGTAPALGINAFFAYSVCLGMGIPWET
                     ALAGVFVASLIFILITIFKLREMIIDAIPADLKYAISGGIGLFIAFLGLSEGGIIVSN
                     ESTLVGLGSLNVGSTWLTIFGLVVTAILLVRRVPGGIFIGMAATTILGLVTGLIPMPS
                     EIVAAAPSLKPTFLVALKHVGDINSLQMWVVVLTFLLVTFFDTAGTLVGLANQAGFMK
                     DNKMPRVGKALASDSTAMLAGSLFGTSPVGAFVESSAGIAVGGRSGITAITTGIFFLL
                     GLFFSPLLSVVTSQVTAPALIMVGVLMAQSLRQIEWGNLEIAIPSFLILIGMPLTYSI
                     SDGIALGFIFYPITMLAAKRGKEVSPIMYGLFFVFVGFMWILNVK"
     gene            complement(39223..39447)
                     /locus_tag="ACG842_000500"
     CDS             complement(39223..39447)
                     /locus_tag="ACG842_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0247064.1"
                     /translation="MDLMGRLEGYLKYRRAKHSEKLRAKNLKSGKKKTKGWGKMVDTG
                     FGGLNNLNPVDTKEFIKEEQKRNGKIKSHY"
     gene            complement(39467..39889)
                     /locus_tag="ACG842_000501"
     CDS             complement(39467..39889)
                     /locus_tag="ACG842_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFQ0247065.1"
                     /translation="MESVYRQERKSNRKKGQMMIQKTRVMLYVNDVEMICRFFVEKIG
                     AEISETIELPEEFKSIVLSISKELELGVFPKVFVQKFSPEVLGPPPSLVFFTDEFETI
                     YENMEEPGEITDNNGILTFNFSDPEGNYFVIGKAESSD"
     gene            complement(40031..40741)
                     /locus_tag="ACG842_000502"
     CDS             complement(40031..40741)
                     /locus_tag="ACG842_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase beta subunit-related
                     protein"
                     /protein_id="HFQ0247066.1"
                     /translation="MKKVIVDTNFWEVFPEATIEILSVNGIDNHVTKESEETYHQLLN
                     EAAKEARNYLTEETFSQNEVIAQWRQAFTTFKTKKGARSSIEALLKRANQEREFFPIN
                     PLVDIYNSISLSFGVPCGGEDIEKIAGNLHLGKAQGGESFLPLGADSDAPALPEEIIY
                     YDEQGAICRCLNWREAQRTMLTEETQAAVLVIESINAEQAARGAQAIEALKEKIDQTF
                     GVTSVRHTLTKDESEAEL"
     gene            complement(40759..41256)
                     /locus_tag="ACG842_000503"
     CDS             complement(40759..41256)
                     /locus_tag="ACG842_000503"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0247067.1"
                     /translation="MTPSDVDLLQQISIETFTDTFGKDNSPEHLADYLTQAYALEKLT
                     EELKDKETDFFLVSIDQEPAGYLKLNTGTSQSEEMADHFLEIERIYVRKKFKHQGIGT
                     FLLDTAIEKAKEKQKTAIWLGVWEHNDVALAFYRKKGFIKYSEHVFYMGDDPQTDYLF
                     IKQLF"
     gene            complement(41293..41745)
                     /locus_tag="ACG842_000504"
     CDS             complement(41293..41745)
                     /locus_tag="ACG842_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFQ0247068.1"
                     /translation="MAEILRDIGMIARALDSISNIEFKEVDLTRGQYLYLVRICENPG
                     IIQEKLAEMIKVDRTTTARAIKKLESNGMIERLEDKENKKIKKLYPTKKGAEIYPFII
                     RENNYSNAVALNGLSDEEAKQLEYLLNKVCKNVSEDWNFVKKGNKRIY"
     gene            complement(41880..42485)
                     /locus_tag="ACG842_000505"
     CDS             complement(41880..42485)
                     /locus_tag="ACG842_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HFQ0247069.1"
                     /translation="MNKKLAQYKRLLALSQAGLYYGKDVFDKERFEEIRTISLELISE
                     IGKETFEEIKALTTIGEGHPTPKVDVRAYIKKDGKVLLIEDKRTKEWSLPGGFAEIGL
                     SPEENVRKEVYEETGLTVETTQLRAVFDTNKQKDIPQLFQYYKLVFACAIHGEEAKFI
                     ENNETSNMGFFSIEELPKLSEKRTTKKQLLILENKNQIYCD"
     gene            complement(42935..43555)
                     /locus_tag="ACG842_000506"
     CDS             complement(42935..43555)
                     /locus_tag="ACG842_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFQ0247070.1"
                     /translation="MKKYFILLAGSPGTGKTYLMNKLRERFPDMYAITLDEIKEYYAE
                     SIGFNNLEERAEQERTKVYPFYYKALDLYMEAGKKVIVSEYPFSDKQKARLQELADRY
                     GYQVITIRLTADFEVLWQRRYKRDRESDRHLSYIMDHYHYGDVLEDRTKGTNHITKEA
                     FKKIIADRKYDKFQLGTLYEFDVTDYTQVDYTPLLDKLEKEINDNK"
     gene            complement(43557..44426)
                     /locus_tag="ACG842_000507"
     CDS             complement(43557..44426)
                     /locus_tag="ACG842_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HFQ0247071.1"
                     /translation="MYTTLKEVTKTAEELNFTVGAFNTHNLEMLPAMLGAAKEMGSPI
                     IIQTSVDTAKYIGMHVLVNVMTAIAEEEMVDAVLHLDHARDFDDIKEAIDSGYTSVMY
                     DGSHLPFKENILKTKAVVEYAHAHGVSVEGELGTIGGTEEGIHVAENDKVYTRPEDAV
                     KFVEATGVDALAIAIGTNHGQFKSKTEVNIPLLKEIDAVVDVPLVIHGGTGVKEEDYP
                     ELINNGIRKFNVGTELLVNWTKVAKETFSETEINKSLRHNVIPANEAVKEIVKHKIGL
                     FMNLESPINLGNK"
     gene            complement(44449..44856)
                     /locus_tag="ACG842_000508"
     CDS             complement(44449..44856)
                     /locus_tag="ACG842_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFQ0247072.1"
                     /translation="MFGFFKKKEKEPKKVLAKEEKALSPEATEKIQSEITQLKQEIST
                     TQDKHKLAKLYEQVGLKFSELYVNDQAIQYLEKSLENKQTIGDGYKKLMSLYNQKRAD
                     AARAGDDQGIDYYMGKMDEMRQIAKQVTIKGNK"
     gene            complement(44875..46164)
                     /locus_tag="ACG842_000509"
     CDS             complement(44875..46164)
                     /locus_tag="ACG842_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFQ0247073.1"
                     /translation="MGVINFIIENILTQASITIALIAMLGLLLQKKSAGQVISGTLKT
                     LLGFQVLSAGSSIIVGSLTYFGKIFTEGFHMQGIIPSIESINGQAMNDLGLGRDIALT
                     FLAIFVFNIILARFTKWKYIFLTGQAILWMATMTTVFGYFAGLRGIVLILVGGFIGAC
                     FAIAMPAVAQPIIRKITGSNDIALGHFCTIGYLFEAGVAKLFGEKGENKKSIEDIKLP
                     THFEFLQDTYLSVMVVMVPLYIITVLFAGEPFASELSGDQNYIMFAFLQAIQFVVGVY
                     VLLAGVRLLLGEIVPAFRGIAMKLVPDAIPALDCPVFFPYSPNAVILGFITTTIGTII
                     AMFTLPMFGLAMILPGMLTNFFAGGTAGIFGNAVGGRRGAIIGGIAHGFFITLLPALL
                     VTIFNSMGFNNATATDVDTVAAALLYAWILSPVLKAF"
     gene            46222..47561
                     /locus_tag="ACG842_000510"
     CDS             join(46222..46663,46663..47561)
                     /locus_tag="ACG842_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEnfa3 family transposase"
                     /protein_id="HFQ0247074.1"
                     /translation="MRKYTFVFKKKVVSDYLNNEGGYKYLAHKYQINRTLVRHWVRIY
                     NYHGWEGLVGGGKSYTTKFKLDVIEYMETNGLSIQETAKKFNIGSNRTLSKWIEQYEE
                     GGASSLESQKRGRKISMNSKLNIPKKLKDESLEEEVIRLRAENAYFKKVRNLDSRTRF
                     EQEEIKVKTILQLKKEFKLNLLLSIAQLAKSTYYYWVKKLDKPDKYSKIKQEITAIVK
                     ESRNSYGYRRVTLALKMKGYTINHKTVRKLMSQMGLTCQIRIKRYKSYKGTVGKIAKN
                     VLKRNFSVDTPNKKWVTDVTEFKIKGRKIYLSPILDLFNGEIISYSISTSPTYKLIEE
                     MLQQAIKKKGTEGSLILHSDQGWQYQMPQYQKKLKENNIIQSMSRKGNCLDNSVIENF
                     FGVLKSEFFYREKFRSIEIFQSKLNEYIRWYNNKRIKLKLNGLSPVEYRKQSIK"
BASE COUNT        13558 a        10026 c         8139 g        15840 t
ORIGIN      
        1 tcattgacag aaatttataa aaagaatatt atgtactaaa ataataaaaa aagttttata
       61 aaaaggtata aaaccttttt attaaactat ttaatcttgt ttagtatata tttttagaaa
      121 aaggagtggg gtttttatgg cagaaatcgt caaagtcaca catttgaaaa agagctttgg
      181 tcgtttcgaa gctttaaaag atgtctcttt caccgtacat tcaggtgaag tcgttggatt
      241 tatcggacca aatggttctg gaaaatccac gacgattcgg acacttttag ggattataaa
      301 aaaagacggc ggaacggcaa cgatttttgg gcaagatgta tgggaagata gtttatctat
      361 ccatgaacgt ctttcttatg ttccaggcga tatctctttg tggggaaatt tgactggagg
      421 agcgatcatc gatctcttca tgaagcttca tggaaaaggg agtgctcaaa aaaaagatga
      481 actgattcaa cgtttctctc ttgatcctaa gaaaaaagca aaaaactatt caaaaggaaa
      541 tcgccaaaaa gtgggcttga ttgctgcttt ggctgttgat tcggatctct atttatttga
      601 tgagcctact tctggtttag atcctttgat ggaaacgatt ttccaagaag aagttgaaaa
      661 attgaaagaa cagggcaaat ccatcctact ttcttctcat attctcagtg aagtcgagcg
      721 actagcggat aaagtcgtga tcatacgcga aggaacactc gatgaagaag gaacactcga
      781 tgaattgcgt catttgactc gttcgaccat cactatagaa acagaagcag agaatagtca
      841 cttagcacaa ttgaacggtg tccatgactt tgtgcaaaaa ggtcacctcg ctcaatttgc
      901 tgctgataac gaagctttcg atgtgatttt agctgaagcg tccaaattag gtgtgaaaaa
      961 atttgaatct attccaccga ctcttgagga tttattcatg agacattatg aaggatgagg
     1021 aggaagcgaa atgactgata aattttcacg ctggtccacc cttttttctc aaaaccttag
     1081 ccgtgactgg aaaaagatcc tgatatggct agtcgatgtc actgcttttt ctggtggttt
     1141 tgtccctgcg tttgaagaat tgacttccgg acaaggatta gctggattat acgaaacctt
     1201 gcaaaacccg gcaatgattg caatggtcgg accaactcca gcttcgaatg ctgctgagta
     1261 tactttagga gcgatgtatg caaatgaaat gttgcttttt tgcagtttgt ttgctgctgt
     1321 gatctctgtt ttacacgtgg tgagccaaac gagaaaaaaa gaagaacttg gtctgattga
     1381 acttattcgt tcctttcgag tcggacgtca agcaaactct ttagctgtca tcgctgaaag
     1441 tattctgatc aatttgcttt tagcaatatc gatcagctgt acgatgattt ttttccaagc
     1501 ggattcgatc actgcagaag gtgcttggct ttttgggcta tctgtaggta gtatggggat
     1561 tttggggagt gccgttgcac tgtttgctgc acaactgatg ccaagttcag ccagttcgaa
     1621 tggtctgtct ttagcgtttt taggtggact ttaccttcta cgagcaataa cagatgtagc
     1681 tgattcctcc ctttcgatgt ggaatccttt aggctggtct tatttgacct atccatttac
     1741 aactaacaac tggattcctt tgctttattt gtttttgttc agtctaatct tgataatcag
     1801 ttcatttgtt ttagaggaac atcgagattt gaacaccggc tatatccccg aacgtgaagg
     1861 aaaaccgtat gctaaacgtt ctcttttatc cttgcgtggt ttattattct atctaaataa
     1921 agggatgaca atcagttggc ttatcacttt tggtttgatg ggggcggcat acggctctat
     1981 ctacgggaat atgcaacagt ttttagaaag taacgactta atgaaacaga tgtttatcca
     2041 atctggcgtc tcaattgaaa aaaactttac aagcacgatc atggtagtct tgatttgtct
     2101 agctgctatt ttaccaattg ctagcatcaa tcgtttattt acagaagaaa cccacggacg
     2161 tatgaatcag ctgttcgcaa caaaaatttc taggaaccaa ctttattgga ccaatatgtt
     2221 actagctatt ctatttgcta tattggcaat cctgattagt tctggtgcac tagggctttc
     2281 tgctttggct tcgatgaagg atgcgaaaga catggatcta ttgatgtttc ttaaagccgg
     2341 gttcaatttc ctgcctgccg ttttagtgtt tatcggaatt agtggctttc tattcggatg
     2401 gcttcctcga atcggtaaac ttgcttatat ctacttagct tactcctttg cactcaatta
     2461 ctttggcgga attttggatt taccagaatg gttttccaaa acagctgtcc ctagctgggt
     2521 tccacggatg ccaaacgatt cttttgatct ttccgtgttc cttacgttat catgtatcag
     2581 ccttgcactg ttcgtccttg gttctattgg ttataggcga agagacttac tagaaggata
     2641 ataaaaaagc atgtacaatt tcatgctagc ctccaaatct gggtcaaaaa tccaacattt
     2701 ggaggtcatt tttttattta caagtcttct aactgctctt tatcaatgat ccagacttct
     2761 cgattctttc tttttacata accattctcc tctaataatt taaattttct agataaagtt
     2821 tcgggggtcg taccgataaa tgcagcaagt tctttcattt tcattggcag gaaaaagtga
     2881 tccttccctg tcgctttagc taagtcaagc agatagacag cgactcgttc ttctactttt
     2941 tccatcatca aaaaagttgt ctgctgctgt acttttgtta atttctcaac gttgattgct
     3001 aaaagtttca gactaagggc gggataatgc agtaagagtt ccgagaaatc tttttgtctc
     3061 aatatgcaaa tttccgtttt ttctagtgct tcgccaaata aagactcatt tggaacgcct
     3121 agcaactggc tctctccctc atatccgccc ggctctatta ctcgtaaaag ctgttccttt
     3181 ccgctagcag ataattgata cactttcata ctgccggagg ccacgatcac taattgaggt
     3241 tctccattcg gcgcaagaat ctgttctcct ttttccacat agtgatgttt gacaagtgcg
     3301 tgtacttttt cttgatcttg ctgtactaag tgattgaaca aaggaacaaa tgaaacacat
     3361 aaatgatcat gactcataat ctctcctcct tctaaacagc tatttctaat aaagaaacat
     3421 aacttccctt attccaactg ataattttct tttatcatag cataaacgtc aagcgaacca
     3481 gataccagca aatagagtgg atttcttttt gtcccttgct ttattttcct tcttttgaga
     3541 ccttaagtaa ttgattccta tcaatttttt aaatatttac tttttgaata atcaaaaaca
     3601 aaccttcact cgcaagccga gagtaaggtt tgtttttcgc attgcttatt tcgtgttgtc
     3661 tattgaaatt tttccgcacc tagcttttga tatagtggtg ctagttcttg taaggtttca
     3721 gcggttattt gctcaacgtg ttcgatttct tgcggataga agaatagttt acccatttga
     3781 aaggttcggt ctctcatcgc ttttttcaac tcttcttgtt tctttgtatc ggacaaatag
     3841 tcagacaaag ctaagtggtc gtcaagttca tgttctggtt gcggccatat gtaataatcc
     3901 ttgatatcga ctgttttgct ccactctttc agtatcgcaa gcaactggtt atacgctgca
     3961 atgctaccat atcgttcttc gcttttataa tgttggtaca tcaaatagat ttggtattgt
     4021 ttttgattca acaaaactgc tagacaggca ttctcgtctt gacgagattc gcttcggtac
     4081 gcgctccaaa aatgactgcg taaattccag ccattcgtcc agctttcgat tttaggtttg
     4141 ctcatgccgt acatatcagg caaaagtgca gctgtctgga gctgaatctc tttccatttt
     4201 tgccagtgtt ctttatattc tttcttgatt tgttcaatct tatctgttgg atggctctct
     4261 ttgagctgtt tgaatgcgta gattggtcga tcaaacaact gataatctga ttttccaagt
     4321 tccattgttt cgcctctttc cgttatcttt tgtattctac ctgaattttg cgatatggac
     4381 aaatagaggt tgtgaaaaag ctgttctgca tcaagtcata agaacagcca aacaaaaaac
     4441 tgcgatgagc atcaccgcag ttttattgaa cttaaggttg attgatcaca tctaaaagcg
     4501 ttgaaaataa aatggcacta gaagcagctc ctgggtcgat atgtccgatt gcacgctctc
     4561 ctagatagga agctcgtcct tttttagcta ccaagacttt ggtgtgttct tttgcctggt
     4621 caatgatttc ttttgttaaa ttccctgcct tcaatgcctc tgtgactggc agccaaacat
     4681 ctaacatcgt tttatcgtca gcttctgcct ttcctcggct ttgaataccc gctgttccct
     4741 gctcgatcac ttcgcctaat ttttcttgag aatcaataga atcgacctct tttgtcgctt
     4801 tcatcatgtt catgaatgca gatccgtata acggaccaga agccccaccg actttcgata
     4861 tcatcgccat agagaatact ttgaatgtat cgctgattgt ttcaggatga tcggcttgaa
     4921 aagcttcttc ataagctgaa acccctcttc ccatgttatt tccatggtct ccatcgccaa
     4981 tcggggtatc tagatcactg agatattgtt tgttctgatt gattgcctct gcaaattgcg
     5041 tgagccattc ttggatttct tgaacggtta gtttcatatt atcacccttt tatcttgaga
     5101 ttttttacca tgaaatcgtc tgtacattgc tttctaaaga atctttccat tgtgtctccg
     5161 tcaagtcgat cagtgttaga gaaagacctt gcatatctag tgaggtcata aaattaccga
     5221 cttttcggaa aactacattg acgcctcttt cttccaacag agctaaaaca tcattcatga
     5281 acacgaactg ttccatcaaa ggcgtcccgc ccattccgtt gactagtaca cctgcttctt
     5341 ttggtaaatt cgagtaatca tctaaaatct tagtaacaag ttcctttgcc agtactttag
     5401 aaggctgcat cttttctcga cgatagcctg gctcaccgtg gataccaaca ccatactcga
     5461 tctcatcttc tcctagttcg aatcctggtt tccctacttc tggtacagtt gctgctcgaa
     5521 gcgctagacc gatcgtctta gttgcctgta cgaccttttc tcccagttct ttcaactcag
     5581 ctagggaagc accgtttctt gctgcatcgc ccacgatttt gtgtacaagg acagtacctg
     5641 caactccgcg ttttccagca gtatacgtac tattttcaac ggcgatatca tcatctacga
     5701 tcacactttc tacttcaata tcgtccatag cagctaattc ctttgccata tcgaagttca
     5761 atgcatcccc tgtataattc ttaacgatca gcaaaactcc ttttccacta tcggcttcct
     5821 taatagccgt ctgaatctga tctggagttg gagaagtaaa cacatcacca agcaccgctg
     5881 cacttaacat cccttcccct acaaacccgg catgggatgg ttcatgtccg cttcctccac
     5941 cagaaaccaa accaacttgt tccgctttct gattcttggc tacaacacgg gaagattcta
     6001 cacgatgaac aagttctgga tagcttttta ctaatccttc cagcatttct tcgacaatat
     6061 tgcttggatc attcatgatt tttttcaatt tattcccatc ctttctttcc ggcattttgt
     6121 gcttctgtta agatttgctg agtatcatct gttataccgg cagtgatggc tgctgcaaaa
     6181 gctccttcca ctaaaggcgc atccaccaga tgataacgtt gctgctgctc ttcttccaac
     6241 atgtcttttg ccagttccga gttcaatacc gcgctaccga tatccgcaaa aacaaaataa
     6301 ctagctgcat cgcttgattc attgactgct tctacgattt tcatcgggtc tgatccgatc
     6361 tcgccattta ttccgccgag agaaaaaatt tgcacctctt cggatttttg catctgctca
     6421 atcatttctt taataccatc tgttactttt tgactatgag aaataagcaa aatacttttt
     6481 ttcacaagaa tcttcctttc ttttacatag ttgctaacga ttggtttcgc tttcattaat
     6541 gaacaattca agtatataat aaatttctaa ttttgggaaa atatgtgtag tgcttcttga
     6601 agtttgttat actttaatta aaagagagga gtgatcgaat gattacaaca acaacgaaca
     6661 gtgtagaggg aaaagccgta gtagcttata aagggatcgt ttttggtgag gtcattactg
     6721 ggatccatgc atttaaagac ttggaggcgg ggttgcgaaa tatctttggc ggccgctcaa
     6781 aaagctatga aaacgaacta atgggtgcaa gagaagaagc acttgccgaa atggccgaac
     6841 gtgcaaaagc attaggagct gatgcgatca ttggcgtaaa aatggactat gaagtattag
     6901 gcagcgacaa tggcatgctg atggtcacat gtagtggaac agctgtctcc acgagataag
     6961 taggggacat ctgttttaat aactaaagag agatgggaca tgtagtggca ctcaagaatt
     7021 agatttttca gtctaacttc gaggtactct accttcccat cctctctttt ttatttcttt
     7081 ttgcggatga ttccgtaagc tgctgcgact aacacacccg cccctgctaa ggaataactg
     7141 acagaagctg ttttttcatt tgtttttggc aaagtctgac ttgttgattt ggatggctct
     7201 gtcgtttgct gaactgtttc tgtcgtgctt ccagaggtca ctgatttttc tcctgccgaa
     7261 tgcaaaatat cttttattcg ttggaaacta gctgctaagt cagctttttt tgcttcaaaa
     7321 tcattagcaa ctgtttgtgc ttcgctgatc tttccgtgga ttgcttgact ggccgctgtg
     7381 atatccgctt gagtttgact tagctttcca gaagctgttt gagcaacctg atttaattgc
     7441 tgattgaatt cttttgcttg ttggatcttc ccgtcaagat ctgtttggac agcttccgct
     7501 tttgctttcg cttcggcaag ctttttctca agttgtgcac ggtcatttcc cactttattt
     7561 ttaagatcag ctaaatcttg atcgattgct gcttggtctt ctttcaattt gttttttgca
     7621 gcttctaatg cttcttcgag ttcttttctt ttattttcca agtcttgcaa atgatcgatt
     7681 tggtcagaag aggtagaact atttattgat gtatcttctg aagatgaaat cgtttctcct
     7741 gaattatctg tcggtacatt ggaagtcatt tctgtttccg aggcaccgtt ttcagcacca
     7801 gaagagctta tggttgttga aggttctgtt tgagcaggtt cagaagtttc cgcaaaagct
     7861 gtctgtccca ataagaaaat actggaaaca gcaattggcc agattaacgg cttcattctt
     7921 tttttcatca cttttcccat ccttactact ttttaactaa tttcttatta taagcaaaaa
     7981 tgtttggaag aaccataaac acaacaggct gtttgtctaa aaaacggtgc atcttttatt
     8041 tttttgtacg ttccgggctt ttccatgtcc cgcttccata acctaaaatc gttttgactg
     8101 ctggaatgaa agtcgtcacg atcgttatcg cgttcactat ccagaacaaa agcatatata
     8161 gtggtgcgaa aagaaggtat ttcaattttc ttcgtcgatc atctacaatc agcgaagcta
     8221 gcaactggaa aaatcctgcg accatttcaa agcagatgaa cacaaaactg aatgtaaaag
     8281 taatataaat ctgttcgtag tttccttgat atacataatg cccgattaat ccagcaaaaa
     8341 gaactacgga aatacaaaag aatattgacc agacaatact caatgtttga tcaatgaaga
     8401 tgatcgttct tccaatatgt ttaaaaggat ggagcatcac ttttttgaaa ttcgtcagcc
     8461 agacttctgt tccacctttt gcccaacgtt ttctgtgtcg atatagcatc ttcaacgtca
     8521 caggtacttc catgaaaaag ataatctcag gagcaaaaac agataaccag tcatttaact
     8581 gatgatccca agcaatactg atgtcttctg tcgctcgatc ttgtctgaat ccgcccacat
     8641 cgatcaaggc ttcttttcga tacatcgtat ttgctccact gtacgcataa agaccaccga
     8701 aaacagctga ctgtgttcgt ttaatgatgc ccacgatact tgagaattca accgtttgag
     8761 attttgcaat caatttggta cgattttgga catccatatt tgcagtgacc gcagcaatat
     8821 gtcttgcacc agggcgaatg aaataattca catacctaat cagtgcatct ggctccggta
     8881 ccgtgtctgc atcattgctt aaaatcaatt tcccttttgc aaaggcaagt ccgatattaa
     8941 acgcgtgggc ttttccttta tttttttcga tccgaaccac acgaagatta gcgtattttt
     9001 tcatcagtct agctaatatt tctggtgttt ggtccgttga accatcgtct gtcactaata
     9061 cctcatagtt gtgataattc aatttcgtca tcaaatactc gattgtatat tcgatcacaa
     9121 tttcttcatt atgggctggc accatgatcg tgatgaacgg ttcgacactc aagggaacat
     9181 ctacccattc tttttgctta tgtttgtata aaaacacata acaccagaca ccaataaacc
     9241 atacaaatcc gcctaaaatc ggataagaaa cgagaaaaaa atacataata gacaaacaag
     9301 tatctaagaa taaattacct gtatgggtga ttgagatcat ttcagttcca ccccattttc
     9361 tttatacaaa tctgcaatga atgtcgtatc tagatttttc tcttcggaga cagaataaaa
     9421 acggaccgtt tctctctctt cttttgttcc aaaacgttcc gtatacactt cttcaagtaa
     9481 ttcttgtctt tttttcaatt tttcatcttc atattcaaaa gatttattta cttttttgta
     9541 tagatttcga ttgttccacc tagtcaatac aaatgaggta atcaggaaac tgaaaaaaat
     9601 caatacaaaa aagccgatta agaaatacag cattttcttt tctgataaaa aagcgtacaa
     9661 ggaaaaatcc ttggctcttt ctggtctcgt taatgggaaa aagacccata taaaaggaag
     9721 aacaacgatc atccatccta aaagagccac taacgtttgc caaatcttta accagtaatt
     9781 accttttgaa aaatacttat ctttcaccaa aattgatgca acttcttctc ttgttacttt
     9841 tttcatatcc tcacctcacg tttttttgtt acgacgtagg agtttaccat acatttcatt
     9901 tcttttcaca taaagtgttc ctaaattttt cctaagaaaa agaacaaaac atttctcatc
     9961 agcaaatgaa aaatattttg ttctttttat cagttgttct ttttattttt tatctcttaa
    10021 ttatgttgct ctttccaata cgacaatgct tttcattcct ctagatatcg ccgtgtacat
    10081 ttgtctgcgt cttctttgct catctccatc gttttcaaat ggatataaga ttacatgatc
    10141 aaattccagt ccttttgcta gactcaaagg aatgatttgg cagttttcta atccatatga
    10201 atttttttta atcgattctt gtagcgtttg ggtttcctct tctgtttggg tgataactgc
    10261 cgctttttct ccttttagag aaaccaaaat ctcagctagt attttcaaca attcttcaga
    10321 ggatcgaaaa gagcggtatt cgggttcttt tccttgcggt cggatcgata cgatgtcgat
    10381 cttctcttga tccacagcat aggttttgaa aagctctgtg attgctccgc tagaacggta
    10441 actatttcta agatcatacc gatggatcgg cagatggtag tcgtcgaaac aacgcataat
    10501 atcagcaaaa gtagtggaag aattaaagat tgcctgattc tcgtcaccta ctaaagtaaa
    10561 gtgagttttt ggaaataatt cgatcaatag acctaattgt gccagggtat aatcttgtac
    10621 ttcatcaatc aatagatagc gaaatgctgg tacatttacc ttttcaacaa gtaaatgacg
    10681 aatagctaat ataatgactg cttcatcaac agtacgtgtg ggttgccaag cataagctga
    10741 gccatatcgt ttttcataaa ttctttcaaa caactgatgc tcatccaccc atgccatttc
    10801 ttcgatttgt ctggttattt ttcgatattt tttttgtaaa agtttccttg cataagctac
    10861 aatactttgt tccgaatcat cagaaatcaa tttaccgaat aattccaact gcctatcttc
    10921 tgtcaacgag agtacctgat tctgcacagc aggacttttt gcttgtttca aaagatgatt
    10981 cttccattcg ctcatcagtg cctttttcgt tgcttgaagc ctatcgatca atttaggatg
    11041 agcaggaact ttctcatata ttttttcgat cgttttttta gaaaggactg tttttccatt
    11101 tttagtaagt ccacggataa aattcggatg atctaaaaac aaaggatctg attgttttag
    11161 attatcgata aactttttgc tacgcaaacg ttctgtctgc tccgatacat tttctcccgt
    11221 gatacgtttg aaataagcct cttcaccttc tatttcctct gctgaaagct gtgaaaccag
    11281 ctgtatgatg gttagattca gtggatttct ttcacctaat gatggtagga cattcgaaat
    11341 atagtcgata aaccgatgat taggtgatag aatcagcaag tcatcggatg tcatcgtatc
    11401 tcgatataaa tagagcagat acgcaatacg ctgcatgata gcagaggttt taccacttcc
    11461 tgctacgcca tttaccaaga tattttgact tttcacatcc cgaatgattg tgttttgttc
    11521 cgattggatc gtagcagtga tagccttcat ttcattggtc tcatcttggc ctaagacttc
    11581 taataatacg tcatcttgaa tagctacaga cgtatcaaaa aacttgacca aacgatctct
    11641 ttccaaaaga aattgtcgtc tttctttaat aaccgtatcg attttatttt gacgaaccgt
    11701 ataagaagat ggaccaatca tgttattgta aaaaagttcg gcaattggtg aacgccaatc
    11761 ataaacaaga ttttcatctg catcatctgt gaaatttgct gtaccgatat aaaaggattc
    11821 ttttggctca ttatccagaa agtctacaac gacttttcca aagtagggac ttttcaataa
    11881 acgttcgatc tttgctagct gattttctgc gtttttctgt ttgatattca tctgatcgat
    11941 ttctctattt ttcatttcaa tcatcgaaaa agtatccagg ttatctaagt aactgtcaaa
    12001 attcaatcga agctcgccag ccatttcttt caacgactgg tttccttcta tttttgccgt
    12061 atccatcgat tcccgcagtt cgtttttttt atcaattagc ttttgataca cgttcttcag
    12121 atatgcttct tctaactgtt tttgtgtgtt gaccatacac attctcctct ttttttaaag
    12181 tggtggaacc gattagttta cctcttttaa gagtcgaatg caagcttttt aacgagtatc
    12241 atttgttagg atatatcagt tcttcaggtc ggacagtttg atataaagaa aatgcaccat
    12301 caaggttttt tacgtggtag ccagactgct tcaagattct ttccgctaga tagctacgta
    12361 atccgctatg acagctgacg atatattctt gttggctgtc taattcattt aatcggtccc
    12421 gaagttcatt caaagggata tgtttggctt ccttgaattt gcccttttcc aattcttctg
    12481 ctgttcgcac atctaataaa atttttcctt ttgctagttc tgtcggcaat tcatgccatt
    12541 gtacggtttc actaagccct tctgcaatgt tcattgctgc atatcctagc atgtttactg
    12601 gatctttggc tgagccaaat ggcggtgcat acgtcaattc taattccggt aaatcgaaga
    12661 tcgtcaaatg ccctttgatt gctgttgcta aaatatcgat ccgtttatcc acaccttttg
    12721 ccccaacacc ttgtgcacca taaatctcgc cagtctctgg atggaagatc aacttcagta
    12781 aaatatctgt tgcttctgga taatagcttg catgatcttt tcctgataca tgaatgactt
    12841 caaaagacag ttcgttttct ttggctgttc tttcacttaa accggtggat gcggcagtca
    12901 ttccaaacgt tcgtataatt gctgttccaa taccgccttg atttcttcga gaaattccgg
    12961 aaatcgtgtc cgcaacttgt ctaccttgac gattggctgg tgaagcaaga gaaataagtg
    13021 catcttttcc agtgatttgc tgttttacaa cgatagcatc ccccaccgca taaatatcag
    13081 gaatattcgt ttgataacgt tcgtctacta ggataccccc gcgtatgcct aattcgattc
    13141 cagcggtcac tgctagctgg ttatcaggac gaacaccaat ggataaaata actaaatcag
    13201 cagggatggt acgcccatca tccagtcgca atcgtttccc gtggtcttca aatccagcga
    13261 cagcatgaga agtaatcact tctactcctt gatgagacaa ttctttttct ataaaagctg
    13321 ccatttcttc atccagtgtt ggaagaatat gtggtgcttg ttcgatcacc atgacttcta
    13381 aacctcttct ttttaggttt tctgccattt ccaatccgat gaatcctgca ccaacgatca
    13441 cggcacgctt tggctgtttt tctaatgcat gcatcacttt atcgatatct ggtacatttc
    13501 taatagaaaa aacattgtcg gcttctgtta tccctggaat cgatggaaca actggttttg
    13561 caccaggaga taaaaccaat gcatcgtact gttcaatgtg tttttgaccc tcatgtacga
    13621 cttctaccgt tttgttttcg ggaaagatgg ctgtcacttc atgatttgga cgcacatcta
    13681 aacaaaaacg agcggataat gattctggcg tttggacaag aagattttcc cgttcactga
    13741 tttctcccga aagataataa ggcaagccgc aatttgcaaa agaaacatac ggtccttttt
    13801 caaaaacaac gatttctgca tcttccatta atcggcgaag ccgtgtagca gcagacattc
    13861 cacctgctac acctccgaca ataacgattt tcatcttatt tccctcctac tgttcgatca
    13921 aaccattgat tcatgccacc acgtacattc actacatcat aaccagattt ctttaattct
    13981 ttagctgctt gtttactcct catccctgat tgacaaatca catagacggt cttttctttg
    14041 tctccatcat atctgtttat actttgtaat ggaacatttt tagctccttt tatatgcccg
    14101 ccccggtatt ctgcaggtgt tctaacatca agtaacgctg gattttccaa aagcttttcc
    14161 tttaattctg ggactgttat cgattttatt gattgaaata gcaaattcat tttctgcacc
    14221 tcatttacat ttttataaat acctcatggg gtatattaaa caggagcttt attaatatgt
    14281 caaccagaat gactcttcat ttttttcact actttttata tttgcataaa ataccgataa
    14341 ggggtattat ctaattataa aaatagagga gaatgtgtta tggagattga taataaaaat
    14401 atcctgaacc gtttgaaacg tacagaggga caaattcgtg gtatccaaaa aatgatcgaa
    14461 gacgaaaaag aatgtatgga tgtcatcact caattaagcg ctgttcgttc tagtattgat
    14521 cgcgtaatgg gcatgatcgt tgcagataat ctgaaaaatt gctttgagaa acctgatagt
    14581 aatccagaag aacaagcggc taaactagaa caagcgatca agatgattat taaaaagtaa
    14641 aggaatgatt tcaatgaaag tcgttttcca tattgatgaa ttagaaaaat ggtcagaaac
    14701 agggaaaaac gtaaaaaacc tgataaaagc ttctccagaa acgacgattg tcgtatctgt
    14761 aaatggcatc gcgattaccg gttatctaga ctctgccaat gcagaattct tagatttgca
    14821 aggagtcgtt tttcacgctt gcgccaatgc tatgcgtgct aatcatattt cagaaagttc
    14881 tttacctgaa caagtaatcg ttgtaccagc aggtgtcctt gatttagttg aactgcaatc
    14941 ccaaggttat gcttatatca agccttagtc agtagaggtt gcgacagaag tgatcagctt
    15001 caagaaattt cggcttattt ttcgaagaag ctacttctgg aaaaccgtga atcaaaggtt
    15061 gtgaaaacgc tgtcctgcat caagtaataa gaacagccaa acaaaaatag cttctcatat
    15121 ttttcgtttg gttgggctta ttaccgcaga tgcaagcttt tgaacaccgt ttaaggaaga
    15181 ggttgtgaaa tcagcggttt gacctaagta ttagagaaaa aaacgggaaa atagctcctc
    15241 atattttgcc gttttttgag ctaataccga aggtcagctg attgaacacc gtttaaggaa
    15301 gagattgtga aaaagtcgct tagtttcgag tattaagaaa aattttgtga aaatggattt
    15361 ctacattttt gcataaattt ggcttaatac cgagaaactg gcttttgaat accgtttaag
    15421 gaagaggttg taaaatcagc ggtttgacct gagtattaga gaaacaaacg gaaaaatagc
    15481 tgctcatatt ttgccgtttt ttgagctaat accgaaggtc agctgattga acaccgttta
    15541 ccaagaggtt gtgagcctac catttaactc cgaatcacaa gaaataattt caaaaacaac
    15601 ttctcctgtt tttggttcgg ttggcttatt atcgagaagt agctactggg agtctattta
    15661 tcaaaaaaag gaatgcgaca taggtcacac ccctttttta ttttctttga aacatctttc
    15721 ggtactcgac agcatacaag acaaaagatt gtaaaagacc gtaagtgatg acggcaaaaa
    15781 tgactctcac ccacagcgtt ggtaattctg ggaaaagcag caaaaggatc atgccaacat
    15841 agatgacagc agtcgctact ttgccatacc acttcgctcc acttaatttg atatttttag
    15901 atagcccgat taatccagct acaaacataa agccttcttt tatcaagaat agaccaaaca
    15961 acagccaaag ccacggtcga taccatgcca tagacaaaat cagaaaaagt tgtgtcaatt
    16021 tatctgcaaa cggatcaagc accttcccta agtctgttac ctgattatat ctccgagcaa
    16081 tgaccccgtc tagataatca gacaaacctg agacaacaat gaccgctcct gctatataaa
    16141 atgactgtct cagcacaaaa taaagataaa tcggcagcaa aaaaatacgg aatatcgata
    16201 aaagattcgg aatcgtttgt atttctttac gccagtctgt gcttaatttc attttatcgc
    16261 cctacctcct cagcactctt ttctttttat tttagcttac acggggaata aaaccaatga
    16321 tttcactgtt tttatttttc tttacagaca ctgatattat gagctaattt gagataatca
    16381 cttgctgttt tcatcgttta ctcttctttc ttcctgtcgt ttgcgcattt cttttactag
    16441 tactttatga gctttttttg atcctcgctc taggtcacgc tgccgtttac gtgttttata
    16501 atcgataaac agactgtcta aatcgtaacc tgtcgggtac agatcttctg ctgaacgcac
    16561 cagacgaaca cgctgacgag gcacgtggat tatttttttg tctaaatata catcaatcgt
    16621 atcttcacct atatcttcat aaactaacgc ttctttttgg tattcattga caaatatccg
    16681 atcacctttt gcaaagagct gacgttcttt tttattctcc ttcctatttg taactgctgt
    16741 cttaggaaga aattcttttt tctgtgtccg ataggctttt tccgccaagt aacgctctgc
    16801 ttgatggatc aatttcatcg acatatccat tttatgagca atccacaaag cttggctttc
    16861 tcctgtttct tccaacccgc aactgatatt taggacgaag tgcttcccga tcgaaagcca
    16921 tagcagccgg cacaaaatct tcatgatctc cggcgaattt cttgatttct ccataatggg
    16981 tcgtggcaat gatcaatgcc ccttgctcat acatagattc catgattgcg atcgctagtg
    17041 cagccccttc atttggctct gttccactgc caatctcatc taaaagtacc aacgtattcc
    17101 tttttatctt tttcaaaata gcagcgatgt tttgcatatg accagaaaat gtactcaacg
    17161 cattttctaa attttggtgg tcaccgacat cgacgaaaat ctcatcaaag acagcaagct
    17221 ctgtcccttc ttttgccgga acttgcatac cgaacatcgc catcaaagtc aataaaccga
    17281 ctgtcttcaa cacgactgtt ttgcctccgg catttgctcc ggtgataatc aagcctcgat
    17341 agtctttacc gatttcaaca tccaatggta ccgcatgctc aggtaaaaat ggatgtctgc
    17401 cttgcttgat aatgatatgt tccgatttat tgattctagg tgtgcttcca ttgatttctc
    17461 ggctaaattt tgcccgagca aagatgatat ctaacaccgt gatcgtttcg atcaataaat
    17521 cgatcacttc ttcgttttct gcgatagcac cagtcaatgc cgccaggact tgatattctt
    17581 cactgatttc ttcagctttt aacaactgat agtgttcatt caatttagaa atcacagttg
    17641 gttcaataaa aaccgtcgtt cttttatttg attcatcgat aatcgttcca tctattttat
    17701 ttttatagct ggctttgata ggaatcgtat agtattcgcc tttttgaaca atcattgctt
    17761 cttggatcat ttctttgttt ttcggatgac gcaagaattt cagtaatttt gactggattt
    17821 ctttttcgat tagttgtaat tgtttgcgta ctttacgcaa attacgtgac gcgtcgtcac
    17881 tgactttatt attttttatt ttctggtcaa tcaactcttc tacatttatc aaatcaggaa
    17941 gatgtttgct ataagcaaac aacaagggtg tctggtattg attcttatca aaaaactttg
    18001 tgatcatccg actgcttcta agaaaatccg catattcgat caaatcggtt ggctgcagaa
    18061 tcaacccttt tttgacttgt tctgtcagtg catcgatcct cggcaacccc ataaatggaa
    18121 catgctgact gctttctaag atcaagcgtg cttctcttgt ttcttcttga cgatccaaga
    18181 cagtttgtag gtttgtcgag accgtcatat cgctgattcg ttttttgcta tagtttccaa
    18241 ttgctcttgc ttggatttcc tttttgatcc gatcaaattg taattgacta atcatttcgt
    18301 tgtccatatt ctctcctttt cttgaagaga tacctgtccc aaaataaata agggcctaga
    18361 gataaataaa aggatagaac acagcttaaa aaagtcatgt cctatcctta ttagaataca
    18421 aagatattgt tttcctgaca ggtaccgttt cataagcaca ccaaataaac tttgtttatt
    18481 ggctattcga cttactgatt agataccagc tacactcaca aaacataccc cattcttcta
    18541 ggtcttattt attttccact taactatagc aagacacatt ttggttgtca aactttagta
    18601 tgttttatag atttacacca ttaatgaata gacacctacc gcgataattg ctccgatcat
    18661 tggacctgcg attggtaccc atgaatatgc ccaatctgaa gttcctttat tagctattgg
    18721 caataccgca tgtgctaatc gaggaccaaa gtcccgagca gggttgattg catatcctgt
    18781 tggccctcct aatgaaagac cgatggctaa gatcaatcca cctacagcga atacgttcgt
    18841 tccaggtgca aattcgtttt gtccaaaagc tagcaaccca agaactaata caaatgtacc
    18901 aattaattct gtgatcacat ttgctggata attccgtata gcaggacctg ttgcaaacgt
    18961 tcctaaaatc gcgctttcat ctttcgttgc attccaatgt ggcaaataag aaagccaaac
    19021 aagaatggcc cctgcaaaag ctcctaaaac ttgcgcaatg atgaaaggaa cgacaagatt
    19081 ccaactaatt gcccctgtca tcgccatagc tagagagact gcaggattca aatgagctgg
    19141 gcccatgaat ccagaaatat agacagccat tgttacagcg agtccccaac caaatgcgat
    19201 gacaatccag ccagaagcct gtgctttact tttgtttaaa ttcacagccg cacacacacc
    19261 atctccaagc aaaactagga tcagtgtccc aataaactcc cccatgattt gagtcatatc
    19321 actgttcatt tatttctccc cctttagttc ccgcagatct gattctgcaa tcaatgcttc
    19381 taaacgttta gtctgttgcg ccttctcctc ttctgtccat tcaaaatatc cagcaattgc
    19441 atcgatcaca ggttgcttga tctcatcaag ctgatctcgt tcaaacaaca gatgatttgt
    19501 acgacgaatg agataatcgc ctggggccaa aaccatttct tcttctaaac cataacgtaa
    19561 ccgagctgac tcagcgagac tcaaaccagg ataaggtgcc atttcttttg ctaattcaaa
    19621 gatacgtcgg gcattagtcc cgtaaaaatc agcgatataa gtagcatctt cttcctctaa
    19681 accggctgct actccttctt ttgctaattc tgtcactgtt tcttctaatt tagttggatc
    19741 gaagtttcca cctgaaatct gatatttttt agagtcgatt tctttcgtct ctattccgta
    19801 ttcttctttt aataattggc gtatcaatcg caaagctcct tctgccatct tacggtaatc
    19861 ggtgattttc ccaccagata aagtcagcaa accatctggt tctctttcta aagaactacc
    19921 tctggaaatc gtagaaggtg ctttttcatc acggctgttt tccaaatggt tcaacacgtc
    19981 ttctacttca gcacgagaaa ctttgttttc cttatattca cttactgtat cgacaacttt
    20041 attgaagctc ttgtctgaaa tcgatccatt atctccacca ttgtaatcag aaccagaatt
    20101 accaatcaac agtggacgaa gtcctgccca gcttgcttcg atatcattca atgtgatatt
    20161 tgcttctgga tagcgatgat tgatcacatc caatagataa tctacatctt cttgtgtgac
    20221 tttcggatcc gtaaaatctc cttggtaatc cgtatctgtc gtaccaaagt aagtcttgtt
    20281 ttctcttgga atagcaaaaa ccatccgccc atcttgcttt cctgtatcga agtatgtggg
    20341 ttgcggtaca ggcagttttt tcgcatctac aactaaatgg atccctttag ttggacgcat
    20401 tttaggagag actggacgcg taaaattcaa gtttcgtact ttatctaccc aaggaccact
    20461 cgtattgatc actaattttg ctttgatttc gatcacttca tctgtcagca ggtcacgagc
    20521 tttgacgcca acgatttgat cgccatcata taaaaagcca accgctttca ttttacttac
    20581 tagataagcc ccatcttcgg cagccttttt gatattatca atcactaaac gggcatcatt
    20641 gttgcggaaa tctagataga cacccgcgcc ttttagccct tcttttttca aaaacggttc
    20701 tctttccaat acttcttctg gtgtcagcgt ataattctca tatttggttc ctgtcacatt
    20761 tgccagtttg tcataaaggt ccattgctac tttgaccgag aacatattga aggttgttgc
    20821 tccttcgccc tcatagattg gcaaaagcat tggatctggt tttgggatgt gtggggcaat
    20881 actttgtacg actgcacgtt caccaactgt gtcagctact acttccacat caaatgtctt
    20941 cagataacga ataccgccat gaacaagttt ggtcgagcga gaggaagtcc cttctgcaaa
    21001 atcctgcatt tcgatcaatc ctgttttgat tcctgatgct gctgactgga ctgccacacc
    21061 ggcaccagtg attccgccac cgatgatcaa cagatccaat tcttcttgct gcattttttg
    21121 aatgctatct tgtcttgtct tgtttgaaaa catatggatt tcctccttat tcaaatgctt
    21181 gggtagcttt tactgctttt ttccaaccgt tgtaaagttt ttctcggcgc tcttcctcca
    21241 tttgaacttc aaatagcttg ccagcttcat aaaattcgcg gatctcgtct gtatctttcc
    21301 aataaccgac agctaatcct gctaaaaatg ctgcccctag cgccgttgtt tccaagtttt
    21361 ctgctcgttg gatcggtaca ttcaagatat ctgtttggaa ttgcatcaga tattcattgt
    21421 ttgctgcacc gccatctact ttcaatacag gaatcttgat tccagtgtca tcttgcatcg
    21481 tatcgatgat atctctcact tgataagcaa tcgactgtaa agtagcttta atgatatctt
    21541 cttttgtcgt accacgtgtc aagccaaaca tagcccctcg tgctgcctga tcccaataag
    21601 gagcacctag cccaacgaat gcaggaacaa cataaacctc atcttcgctt gtagagcgtt
    21661 tcgctgcgtc ttctgaatcg ctagcttttt gcaacatttg aagtccatct ctcagccatt
    21721 gaatcgaaga accagccaca aaaatacttc cttctaaagc atagtaaacc tttccattta
    21781 tgccgtaacc aattgtcgtc aataaattat ttttggataa ttgcggtttt tcaccggtat
    21841 tcatgacaat aaatgaacct gtaccataag tattcttgac catgcctggt tcgaatgcca
    21901 tttgtccgaa taatgcggct tgctggtctc ctgccatccc tgaaattggt gcttcgcttc
    21961 cataaaaatg ataaccttgc gtataaccat aaacttctga gttagaagtg acttttggta
    22021 acatcactcg aggaatattt agtaaatcta aaatctcctg atcccaatct agctgatgga
    22081 tattgaacaa catcgtccga ctggcattcg agtaatcagt aacatgtgct tgtccatcgg
    22141 tcagtttcca tactaaccaa gaatcgatcg ttccaaacat caattcgcct ttttcagctc
    22201 gttcttgcgc gccttctaca tgatcaagaa tccagcgtat cttcgttgct gagaaatagg
    22261 catcgatcac taaccctgtt ttttcgtgga aaaattcttg atagccttca ttttttaagt
    22321 tatctgcaat tccagctgac tgtctggatt gccaaacaac tgcatgatag attggacgtc
    22381 ctgtatcttt ttcccatatt actgttgttt cacgctggtt cgttatgcca atcccagcaa
    22441 tctgatttgg tttgatccct gattcgatga acgctccggc aataactgac tgtacagaat
    22501 tccagatttc atttgcatca tgttccaccc aactttcttg cgggaaatat tgcgtgaact
    22561 ctttttgaga actgccgatg tgtcttcctt tcttgtcaaa gataattgct cgtgagctag
    22621 ttgtaccttg gtcaatcgcc atgatatacg ttgattctgt cattgtgatt ccctccatta
    22681 tatgcaaacg ctttctttaa ctactcttat catactactg ttttaaaaaa agaaactgtc
    22741 aaaattttat tctttttttc tttttttgac agttttttat tatgaagcta ttttttaatc
    22801 aattcttttc ttttttcagc gtctctattc ttcttttcaa tcgaataatg tcgtaaggtg
    22861 acgcaaattc tgacagataa tattcacact gttttttgtt tctctggcaa aatgtgatca
    22921 ccaaatcata ttctttacct tcttcataac ttccaatctc aataccgccg attcccctca
    22981 gttctctgat cagatattgt tgaaaagcaa tacgataaat cggcagactt tctaaatcga
    23041 ttccaatcac tactggtttt gcaatataaa aatcgatcat caaaagaaca ttggcatact
    23101 ggatcatcag gtaactaagc tcactatctt cagctctttt cctcggcaat tgctgcaccg
    23161 ctgtttcctg taatttcgcc agtaactgtg ttaaggaatg cccgagcatt ttctgctgct
    23221 gttccaaaag ccgatcacgc tcatagactt cgatctttcc aacaaagaaa aaccattcat
    23281 tgttgatctg ggaaatttgg tagcctactg ccttttcttc tttgattttc aaacgacgag
    23341 gacgataatg ccataacatc gtttcccgaa tgtaagtatc taaaacagcg gttggtaact
    23401 ttttgctccg tgtcaaatca tactgataaa atatttcttc gtctactatc gaaaaagaaa
    23461 caaaaaagct gtaaaacatc attgtttcgt atacattcag ttctgctgct gttcgactaa
    23521 gatgcatcac gatgattgga tcgattgctt gataaagccg gtccgactga tagagtaatt
    23581 tcttttcttt caaagttgcg tacgtacttt tttcattcaa tagtcttttt ttcgtaatcc
    23641 cgagccagca agcaatctgt gcttctgctg atgccgtaaa cgttgtttct aatacacggt
    23701 ccaatcctag aatgaacggt cttttttctg gtgtattttg caaaaaaagc ggtcgctgat
    23761 cttccgggat atattggaat agttcataat aaaaatatcg tatctgtagc tcttctccgt
    23821 ataattgacc gtttttgatt tgtaatccaa attcctctaa gagctggttc aattctttga
    23881 tctttcgaaa aacagaagac tcactgatat taaacgctgt cgctaatcga acgatggata
    23941 cttgctgatg ttctaataaa tagacgagca tcttaaactt caagctttcc tgcaataaaa
    24001 acaatagaat tttttccaaa cttacatctt cgtccaacac aagcagcgcc tgatactcat
    24061 ttcgctgtac ttctactttt ttttccatca ttcggccaat caattcaata tcttccaaat
    24121 attgatcaaa cgaggctttt gacagttgga catgctctct caaaccattg atagaggctg
    24181 ttcccccttg cagaacaagg tttcgcaaaa tcaggatctg taactgctct tttctttcta
    24241 ataatgcttc aatcttcata ggcagtattc gccacctttg accacaccat atctggcaca
    24301 cggtattctg gctggaaaaa tagctcatgt tcttctaact gttcaaacgt ccatccttct
    24361 tctactgcaa ttgtcagttg gtcaagaagg tggaacatct cttttttgct ttggagctgt
    24421 gctccaagaa tctttttttc agaatcaaat agaaatttga gtttaacttt gctttgattt
    24481 attgctgtaa gcttgtagtc tacgtatcct gtttcaattt tttggggata aaaaatgctc
    24541 tcttcttctg tcaaacctac gcttcctaag aaatagccaa accactgatt gccgactgga
    24601 cgataggttc cctgatcctt tgtctgtgag ccactaattg tcttgctgac atttcgtgct
    24661 gtacggacag cgttatttac tagagaaaca tacatttttt catttgttgg acgaaaagtt
    24721 acttggatcg catcgccaat cgcataaacg tctttttgag acgtttgcaa gtagtcatct
    24781 acccagatcg ttccatcatc attcaatgtt aattgttctt cccacatctg attgtcggga
    24841 cgagaatgat tagccagcaa aacaagatca tagttctcct ttcttttttc cgtctctaca
    24901 atagcggttt cattttcggt caaagacaca acttgttctt ctaaaaaaag tgaaagccct
    24961 tgatttttca tttcctttac taacggttct gtcatttcag gatctagata acgaaaaaga
    25021 attgactttc ggctttcgta aagatggatt tgtttttttc ttgatgccaa cccttcagct
    25081 atttcgattc ctgcttgtcc tgcaccaata attgcaattt tctttgcatt catgatcttt
    25141 tgctgaagag ccgttacgtt ttgtatcttt ttaactgggt gaataagcgt tgattctgca
    25201 gaaatatttc gaaacttttg atttgaacca gtcgctagaa tcaaacgatc aaaatccaat
    25261 ttttttcctc ctgctaaaac gacttttttt tctttcaatc ctattactgt cttttctgtg
    25321 taaacatgga ttcgatagcg gtcagtcagt tcttctttcg tgatccaaga aagttgtttt
    25381 tcactggtaa cctttccttt taatatcaaa ttgaacccgc tgggaatcca tcctaacttc
    25441 gattgctttt caatcaaata tatttcactt gttggcatca attggcgaag ataaatagcc
    25501 gcatgaatcc ctgcaaacga cccgccgaca attacaatgt tcatctgcat tcctctttct
    25561 tataaggtgt tctactgcta acttctcaat cagaaaacac agtattcata actcgctttc
    25621 aaccttatga tacatagcga tgcagcagaa tcaaccctct ccatcatacc gtgttctagg
    25681 tctgcttctc aataatgtac tagtggacct aattgaagag tatcatttct gaagagatgt
    25741 ttcaaaaaat gtactcttat aaaagaaaaa aactcgcctt ttacgtacct ctgtccaaat
    25801 aggaaacaga aaagaacgaa aagacgagtt ttagcgtgtg taatgtaata gttagaatag
    25861 acacatcatt tatttttaat tctttaatgc ttcaacaact gcttcgccta attgatgagc
    25921 agattgagga ttttgccctg taagaagccg cccatcaatt tgaaccactt ctgaaaaagc
    25981 tgctccagac tgatagtttg ctcctttttc tttcaaagca tcttctgcta agaatggtac
    26041 ttcattcgtt gtaccatttg cttcctcttc ttcattactg aacccagtca ctgtacgtcc
    26101 agctaaaata gattttccag actcatctgt aactggcaac aatcctacaa caccgtgaca
    26161 aacagctgag accagccctt catttcgata gatttgtgaa gcgactttcc caagatctat
    26221 tgcttctgga aaatcccaca ttgttccgtg gccaccagca taataaaccg ctgcataatc
    26281 atttgcatcc acatcatcag gacgtaaggt atttcccaag gcccgatttc tgaaatcttc
    26341 atcttcataa taggcccaat cgatttcatc catattcaaa acactttgcg gttctaaagg
    26401 tacatatccg cctttaggac tgacaaaatc tgcatcgatc ccatttttta ctaatacatc
    26461 ataaaaatga gtcagttcac ttaaccacaa acctgtagga cgctcgatct tttcgtactt
    26521 ttctacattt gttaatacga tcaatacttt cattttccca tcctctttct ataaaaattg
    26581 cgttattgat aacgtgcttt caagaaatta tataatgggc ttggcaaata aagctcttta
    26641 tatgcttttt caaaaggttt cgttacaaat tcatcctcac ggttttccaa taatttctct
    26701 gcccagtcag gatcgacgat catttgctgt ccaattgctg caagttctgc atatttcaat
    26761 acctgctcta catcttcgcg attgcggatc ccaccgacac caactaaagg gatctttcct
    26821 tgaatcgtgt tatggaccac ttcaagaatc gatttatcgt tgtatttatc agaacgagct
    26881 attcgatcat acatatttaa tgaaacatgc agataatcta gttttgactc acgtaatcgt
    26941 tcaagaagcc agatcgtgtc ttcaaaacgg atacctggtg tttcatattc ttctggtgag
    27001 aaacgatacc ctacgatgaa tggtttttta gcatattgtt caaccgctga gaaaaccgtt
    27061 gttaataatt cttcaataaa acgataacga ttttccaagc taccgcccca ttgatcctct
    27121 cgtctattcg aatgcggaga gaaaaattgt tggatcaaat acgtatttgc tccatgcaac
    27181 tcgattccat caaatcctga ttcaatcgct ctacgtgtgg ctttgccaaa tgcttggatt
    27241 gtttcaacga tttcttcttc actcattgct cgtggtgttt ccgcatcagg acgttttgct
    27301 gcaactgcac ttgcagatac aggctgttct ccagataaag ttgctcgact tgtcattcgt
    27361 ccaccgtgga aaatctgaat aatcgctttt gcgccttctc cttggattgc tttcgctagc
    27421 tcacttaatc gaggaatcat ttcatcatga gcgatgctga gttcacctgg ccagcctttt
    27481 ccgatatctt ggacatttgc agcaccagta ataacagcac caagaccttt agaacgttgt
    27541 ttataataag tgatctcatc ttcggtgatc actccattga aaaagctgag ttgtgtcgtc
    27601 ataggcgaca gcatcaatcg gttcttcaat tgaactcctg aaggtaaagt aattgattga
    27661 tcaaattgtg acattgtcat tcctcccata attcgctctg attaacctat cgagaatcat
    27721 ctatcattaa accactcgat tagttgtaca tgcaactaat ttgcttaaaa aattttattt
    27781 tttttgtaac ttatctgttg catcattcat tgtctgggct aattctttac ttgcttcatt
    27841 tcccaaaact gctttgtatg actgatgaag ttcttcccag ctaccatcga cttccttagc
    27901 aaattggcgt ccttgttctg taagataaac taatgacaag cgaccttcgc taactgtttt
    27961 gacgtatcct ttatattcta atttttcaac aaaacgagca atcgttgatg gggcggaata
    28021 gagcatttgt gcaatatctt tttgtggtat tccatctttt tcttttaata tcaataaaat
    28081 aaaaccataa gttggtgcca gacctgtcct tctaaacgct tcatcagcta atttattcaa
    28141 catgcgatct agctttttca cgctaaagaa aagacattca tctaacgctt tttcaattgg
    28201 cttttccatt tcatcccctc cattggcgta catatcatag tatgattttt attttttgtc
    28261 aaaaaacacg gaataatgtc cgcctcctct gtctagattt gaaaaaaaga agtagccatc
    28321 ttcaaagaat ttcggcttgt tttcgacaaa actccttctg gaacaccgtg aatcagaggt
    28381 tgtgaaaaag tcgtttagtt ttgagtatga agaaaaattt tgtgaaaatg gctttccaca
    28441 tttttgcata aatttggctt aataccgaaa aactggcttt tgaacaccgt ttatatcaga
    28501 ggttgtgaaa aagccgtcct gcatcaagta ataagaacag ccaaacaaaa atagttcctc
    28561 atattttgtc gttttttgag ctaatgccga tggtcagctg attgtacacc gtttattgag
    28621 aggtcgtgag cctgccgttg aactccgagt cacaaggagc aatttcaaaa aacagcttct
    28681 catgttttat gactatggtg acttgtgatc gaggagttgg caggagaata ctgtttattc
    28741 gtaaagaagg ggctgtgaca agacttttgt cacagtccct tatttccttt tattatagga
    28801 tttcgccatt tgattggatg acttgtctgt accaatcaaa tgattttttc tttaaccgtt
    28861 tctttgtgcc attcccttcg tcatcttgat caacatagat atatccataa cgttttgaca
    28921 tttgtgaagt agaagcgctg acgatatcaa tgcagcccca cgacgtataa ccaaataaat
    28981 ctactccatc tttgatagct tccttcatct gctcaatatg ctgcttcaaa tagtcaatgc
    29041 ggtaatcgtc ttgaacggtt ccatcttctt ctactttgtc tatagctccg agtccgtttt
    29101 cagctatgaa cagtggtttt tgatacctat catataattg attcaaagca attctcaaac
    29161 ccttaggatc aatctgccag ccccattcac ttgatggaag atacggattt ttaattcctc
    29221 cagttattaa gtttccttgg accccttccc aattttctgg atgagcactg actgcactag
    29281 acatgtagta gctaaaagaa acaaaatcaa cagggtaggc tgctaagatt tcttcatcac
    29341 ctacttctat ttttaagttg acaccttttt cttcaaacat tcgttgcgta tacgctggat
    29401 aatatcctct tgcttgaaca tcactaaaga acaaagaagt tctttgctgc tcaattgttt
    29461 tttgaatatc atccggatca caagaatacg gaacaagaag ctgatagctg atcatacaac
    29521 cgacttgtga acctttgata atttcatgac aagcttttgt agctaaagca cttgctataa
    29581 actgatggtg tgcaccttgg tatttcgctt gttccaaatg gggatggttt tcttcaatta
    29641 ctcctacgct aacaaaaggg tgatgtttta cacagttgat ctcattgaaa gtcatccaat
    29701 attttacttt atttttatag cgtttgaata ctgtttcagc gaaacgaacg taaaactgaa
    29761 taagctttcg attaggccat ccaccatatt ctgtaaccaa atataacggc atttcataat
    29821 gggaaagaga aacgactggc tcaatcccat attttagaca ttcgtcaaac acttgatcat
    29881 aaaaaaataa tcccgcttca ttgggtgttt cttcatcacc atgaggaaaa attcttgtcc
    29941 atgcgataga catccgatag catttaaaac ccatttcagc aaataaacgg atgtcttctt
    30001 taaagtgatg atagaaatca tttccgtgtc ttttaggata ataatacttg ctgttgggat
    30061 cctgcgccgc ttttacttgc tgatgcgtca tcatatgcca ttgatctgtc catttttctt
    30121 taggtaaatc gctgtcgaat gtatacacat cagacacaga taatcctttt tcatctacat
    30181 cccatgcacc ttcacattga ttggccgctg tcgctccgcc ccataaaaat ccttctggaa
    30241 attttttcat atcgatcccc ctaaacttgc tagttttctt gtttgattat ttttcttatg
    30301 actagtaatg atatttttga atctactact atttgtaaca ataaccattg tggtcggatc
    30361 atatccctct ttgattaatt cttgcaaatc tacttccatc aataaatcgc ctttacgtac
    30421 tttttttcca tcagatactg cactttgaaa gtatttgcca tttaaattca ctgtatcaat
    30481 accgatatgg atcagtatct ctgctccttc ttcactgact accccaattg catgtttcgt
    30541 tggaaaaact gttgaaactt ccccgtttac agaactgtag atttttccgt ccgttggaat
    30601 gatcgcaaac ccttctccta aagatccact agcaaatgtt ggatcgttta cttcagataa
    30661 atcaattatt tcacctgctg caggagtact taacactata tcctttgagg attcttcttc
    30721 gatttcatcc ttgtagccga aaagaaaagt tagcatacag gaactgatca tcgctaagaa
    30781 ataactagca actagccaaa caaatccttc accaatataa atcgggaaag acaaaatact
    30841 tcttgtagca actgccaaag caccggctcc tgcggcacca gctactccac caaatatagc
    30901 tccacatata caagcaatgt aaaatggtcg tttatattta agagtcacac cataaacagc
    30961 aggttcagtg attccaaata atgctgtgat aaatgcagat aaagataaac tttttacttt
    31021 cttatttttc gttttcaaaa ataccccaag tactgctcct gcttgcgcag ctacggcagt
    31081 catacatgct ggacctagcg tatcacgacc gtagaaagaa agattgttgg tcattactgg
    31141 tacaagtccc caatgtaacc caaaaatcac taacacttgc caaaatccgc cgataacagc
    31201 accagataaa acagggctaa agttataaac agctaaatat ccagctgcaa tcagctgact
    31261 tgcataatct gcaattggac ctacaactaa taaaataatt gggaccatca cgatgataca
    31321 aatgaatggt gtcaataaat ttcgaatact ttcgtgcaag cttttgcgaa tcttaggctc
    31381 aatataagaa agaacccata cagctaaaat tgctggaata accgtacttg tataccttgc
    31441 tagaatcacg ggcatcccta gaaatcgcaa ggttatgcta tcactatatg cactgacaat
    31501 cgtaggataa acgagagctg ctgcaataac catagcgatg aaacgatctg tattgaactt
    31561 ttttgcagca gtatatgcca aaaatatagg caagaaatag aaaaatgcat cagctgctgc
    31621 attcaaaatg atataagttc ctgattcagt agttaataaa ttcaaactcg tacataaggc
    31681 gagtactcct ttgatcaaac cacttcctgc caagacgcct aaagtcgggg caaaaacagc
    31741 ggcgatcatg tctataaaag agtttaaaat gccttttttc tcagttcctc tctttccact
    31801 agatgcatca tcttctagtt tcatttgttt ggataatacg tcatacactt catttactgc
    31861 atttcctact acgacttgaa acattcctcc agcttcgact acagtgatca caccttctag
    31921 ttgctctacc ttctccttat ctgccttctt attatctttt agggtaaagc gtaaccttgt
    31981 tgcacaatgg taaacattgc tgacattttc ttcgcctccg acaaatcgga caatatcttt
    32041 cgctaattgt gaatagtcca tttttacttc ccccttgtta attaatgccg tagcaatcct
    32101 tcttacaaga taagtatagt tcttagaaaa cggtttctcc attcaattag taccccttct
    32161 aatagcgaac gaaactgtac aatagtgtgt atggtttact tagccgagca aatcactcac
    32221 tgcttgaaag aaaacaagta tatcgtttct cttatagtca aaggagacaa aaaaaacacg
    32281 cttgatcttc ctttgctgat gtataagtgg tatctcaagc tgatactctg tgatttttgt
    32341 ttctacttgg cttttgataa gacagcacaa caaagtctcg gcatttaacg gaagcataac
    32401 taggtcagaa ctaggagaga ttagttgctg aatggaatct atctttttat ggatttttat
    32461 ttgattttct tctttgtagt aatgatcaag cagttttgtt ttcttttttt cttcaactat
    32521 ggtcagcttt tcacgcaaaa tctgataaat ctcccaagat tcaaactcat ctagaacact
    32581 aggtagataa atcaaatcat cccactcaaa aataagctct tgctcagtag tcaaaattgc
    32641 atcatattcc gttattttgt ttgattgatt tttgaaaaga tacactggtt ctatccggca
    32701 ttctttgatc cacatgttca tactttgttg aatctgtttt tttagtgtat tcaaaatact
    32761 aattggctgg ttactaacaa tcagtatctc cccttgtttt cgaaaaggat tcaacgcttc
    32821 ggcaagatac aaaacaaaat aagcgctttc agctaaattg ggacgtaatt gaaaatgtat
    32881 tgggaagaaa cgccgaatta aataaatttc taaaggataa cgggagatga tcttttgtac
    32941 aaaatgattc atggtattat gtccggcatc catcctcgtt ttcatttgtt tgatatgggt
    33001 tagcaaaaat tctgtttcat ggaacaataa cgaaacagga agatctaaaa aacgaataat
    33061 cgtttgctca aatgctctta gattcttctc tatttctttg tttttttcat cattaatcat
    33121 caaataattt aactctaata gtcgattttc aattgcttgt ttttcttgat tggaaaaaga
    33181 atagggtagt tctgatgata acttttttgt gagggtctct accatcggaa taaataatgt
    33241 atacctgttt gttttttcta aaagttcttc tcttcgaaga atggtcaaaa cgagataccg
    33301 agaaaatcgt atgaagtcct ctcctgaaac aatataatta tatgatataa gaacctcttt
    33361 taatgtcttt tggacagtcg gcatgatatt ggaaaaaatt gtactgattt gttgaggaaa
    33421 ccttgcgtgt tggatcaccg tttctgttcc aacaagagat aagaaaattc tttttgaatt
    33481 ttcttcagca atgattttta gtccttttgt cgacgaaact tccagttcta atttaggatt
    33541 gcgctcgatc caacgcaaaa tcgtttttat ttgtaatgat acggctgttt ttgaaagaaa
    33601 gaaaatgtct gctatctttt gcatactgac ataccctttt tgaaacaata agtacgtcac
    33661 aatagttgaa gctcggaaat ccatgctgga atagatttta tgtttcgtca cctcttgtat
    33721 gatttgctgt ttagtttcag attcaattga ttctaaaaaa taacctgaac gtttatttga
    33781 ctggataatg attttcttct cgtagatagc aagcatattt ttgatttcag tttggatcgt
    33841 tttcttgttg gtacctaaag cttcagctaa ttcttcgctt gttatccaat gattcttatt
    33901 tcttaatact acaagaattt ggagttgtcg tttatttaag tccatttgct tacacctcct
    33961 ctttgttata aacatacagg aaaaacctct caaatgacaa tagtcacttg cagaggttta
    34021 aaaatcattt gaagaaagat ctatattcaa ggtttttacc attagactgg ataatttgtt
    34081 tataatattc aaaactatct tttttcaatc tcctaaagct tccgctccca tcattttgtc
    34141 tatccacata aataaagcca tatcgcttcc tcatttcccc tgttcctgcc gatacgatat
    34201 cgattggtcc ccaccataag taaccaatga tttggcaacc atcttttatt gcctcagcaa
    34261 tttcctgaag atgtattttt agatactcac aacgttttgg atcgtggatt cgattcaatt
    34321 catctggtgc ttcatctagt ccaatgccat tttcaactat gaataatggt aattcataac
    34381 ggtctgtcaa ttcattcatg acataacgaa accctagagg atcaattggc caactccaag
    34441 gttcgggaga tttttccttc aaaaagggat tatcgaaacc atttgctccg ccagtatcta
    34501 cgcgcatttc tgcttctttt ttatagacag cgctacgata ataactaaat gcaatataat
    34561 ctgccgtgtt agcttgcaag cactgttcgt ctccatctaa cattactggt gcacagtctt
    34621 cttccttcca tgtcctgtaa atataagctg gataatgccc tcggcacatg acatcggaga
    34681 aaaaccatga attgcgttta aattgcagtg aaccaaatac attctctgga tcacagtcat
    34741 atgcataagt cgctaatgca gaaaagctac acataatacc catcttagca tttggatcga
    34801 ttttctttaa taattgagta gcacgggcgt tcgctagaaa catatgatga caggcttgat
    34861 aaatcacttt ttttgttagt gtagcaatgg gttcaagttt atctctagca gggaatgtaa
    34921 ccaatccagc agctgcataa ggcatacgaa aagcattatt gatctcatta aaggtcatcc
    34981 aatatttcac tttcccttgg tagcgttcaa agacggtctg cgcataaaat tcaaaaaagt
    35041 cgattaattt tcgattttgc caccccccat actctcctac caatgctaag ggggtttcat
    35101 aatgactaag agtaattact ggctcgatat catatcgatt taatgtattg attagtctgt
    35161 cataaaatac gagaccagcc tcatttggct tctgttcgtc tcctcttgga aatattcgtg
    35221 cccatgagat gcttgttcga aaagctttta aacctaactc cttcagtaaa cgaatatcct
    35281 cttcaaatcg atgataaaaa tcaatcgctt catgagaaag ataatggagt gattcgtcta
    35341 aagcaatctc ccaaatcttt ttttcttcat tccactgaat actaggatgc tggttcatga
    35401 tcccgataag cgtatctgtc acattaggta atttgccgtc ttccaaccat gcaccttcac
    35461 attgattagc agctactgct ccgccccata aaaaattttc tggaaatatt tcctgatcca
    35521 taactatctc tcctcgctat caacaatttc ttacttgaag tatagctcga ttcatcaaaa
    35581 caagatattc taactatttc ggcagaagag aagaaaaaaa ttgaatgcta ctcttcattt
    35641 ttaatattta tatataacaa atacataaga cgatccccga aagaaaacac tcgttaacca
    35701 tctaagcaaa cgatttattt tacttccaac acaaatgctt cataaggtcg taaatcaact
    35761 ttgccgtctg gtaactgttt cagaggataa tttgacacaa taacccttaa agcttcacgt
    35821 ggaatagtga acgtctgctt ttcactggac atattacaaa taacgagcca ttgctggtct
    35881 tcatatatgc gagaataggc aaagacatgc tcaggcgtct tttccactaa ttcatagtct
    35941 ccccagacga tgatgtcatg ttttctacgc aaatcgatca atttcttgta catataaaaa
    36001 acagaatctt gatctgctaa cgcttcttct gcattgattt ctttataatt tggattgact
    36061 cgaagccatg gaattccagt agtaaaccca gcatgttcac gggcgttcca ttgcataggc
    36121 gtcctagcat tgtctcttcc tttcgccttg attgattcca agatttcctc ttttgtaaat
    36181 ccagaagaaa tcctttcgtt atacatattg attgtctcaa tatcttttgc ttccgcgata
    36241 tcatcgattg ggcaattcgt catgcctaac tcttctcctt gatagatata aggggtgcct
    36301 ttcatcatat ggagcaagat agcaaacatc ttggcacttt cttttcgata cacatcgtca
    36361 tttccccaac gagaaacaat tcgcggaaga tcgtgattat tccaaaataa actattccat
    36421 ccttcatcac ctaaagatgt ttgccatttg gataatactt ttttcagttc agagatactc
    36481 aagggtttca aatcccattt gtctttgcct tcttgctgat ctaatcctat gtgttcgaat
    36541 tggaagacca tggataattc atttctttta ggattggaat aaagtttagc gatttcggta
    36601 gttgctcccc atgtttcgcc aacagtcatc agatttttgt caccaaaagt tgcttgattc
    36661 atttcctgaa gatactcatg cagtttaggt ccattacttg tgatttcttt atccggttct
    36721 ttgccaatca tatcgatcac gtccatacga aagcccccaa tacctttatc gatccagaaa
    36781 ttcatcattt gataaatctc ttggcgaacc tgtttatttt tccaattcaa atcaggttga
    36841 cgcttgctat acaagtgcag aaaatattgc cccgtttttt catccaactg ccaagcagaa
    36901 ccactgaata ttgagcgcaa tctattaggc tcttttccat tgaccggatc acgccaaata
    36961 taatagtccc gataaggatt ttctttattt ttacgggcct caatgaacca cggatgttca
    37021 tcagaagtat gattcaccac cagatccatg atgatacgga tatttctttt ctttgcttct
    37081 tcaatcagtt cttccatttc ttccatcgta ccgaattctg ccatgattgc ttgataatta
    37141 ctgatatcat acccattgtc atcattcgga gactcgtata ctggagaaag ccaaattgca
    37201 tcaataccta aatacgccag ataatccaac ctggaaataa tcccccgcaa atctccaata
    37261 ccatcttgat ttgaatcttg aaaacttctt ggatagattt gataaacaac tgaatggtgc
    37321 caccattttt gttccatgat tttcctccta taaatggatg tttctatttt ttgcttaatc
    37381 atttatttta gtctctctca ctaaaaattg taacgctttc aaatagctat tgcaccttgt
    37441 tatcggtaac agaataaaac aaaaaaacag ctgaggaaat tccctcaact gttttttgaa
    37501 aggattgatt tatgcaggga agaatgctga tttcacaact tctttataaa cggtgttcac
    37561 tcagctgtcc cttggtatta gctcaaaaaa cggcaaaata taagaaacta ttttttcgtt
    37621 tttttctcta atactcaggc cagaacgctg atttcacaac ctctttattt cacattcaat
    37681 atccacatga atcccacaaa tacaaagaac agaccataca tgattggaga tacttctttt
    37741 cctcttttag ctgctaacat ggtaattgga tagaagataa aacctaatgc aatcccatct
    37801 gaaatactat aagtcaaagg catgccgatc aagatcaaga atgaagggat cgcaatctct
    37861 aagtttcccc attcgatttg tcgcaaagat tgtgccatca atactcctac cataatcaat
    37921 gctggtgcag ttacttgtga agtaacaact gatagtaatg gagaaaagaa caagcctaac
    37981 aagaagaaaa tccctgtagt aatagctgtg atacctgaac gtcctccaac ggcgatacca
    38041 gctgatgatt ctacaaatgc accaacagga gaagtaccaa ataaggaacc cgcaagcata
    38101 gctgttgaat cagaagctaa agctttccct acacgaggca tcttgttgtc tttcatgaaa
    38161 cctgcttggt ttgctaatcc aactaaagtt cctgctgtat caaagaaagt cacaagcaag
    38221 aatgtcagca ctacgaccca catttgaaga gaattgatgt cccctacatg ttttaatgca
    38281 actaaaaatg ttggtttcaa acttggtgct gcggcaacaa tttcagaagg cattggaatc
    38341 aaacctgtga ccagacctaa aatcgttgta gctgccatcc cgatgaagat gccacctggt
    38401 acacgccgaa caagcaagat agcagttacg actaatccaa aaatcgtcaa ccaagtagaa
    38461 ccaacgttga gtgatcctaa tcctactaat gtagattcat tcgaaacgat gatcccacct
    38521 tcgctcagac caaggaaagc gataaataga ccgataccgc cagaaatcgc atatttcaaa
    38581 tctgctggga tcgcatcaat gatcatttcc cgcagcttga agattgtgat caaaataaaa
    38641 atcaaagaag caacgaatac gccggctaat gctgtttccc atggaatccc cattcctaaa
    38701 caaacagaat aagcaaaaaa agcattgatc ccaagagcag gtgccgttcc aattggataa
    38761 cgggctaata cccccattaa aacacagccg atcgcactgg ctaatgcagt cgcagtaaac
    38821 actgctcctt catccattcc agaagcacct aatacagttg gatttacaaa taagatataa
    38881 gccattgaga taaatgttgt aaatcctgcc agtacctcac gttttaccgt ggtgttcaat
    38941 ttttctattt cgaagtagga aagtattttt tccttcatct tccatccccc taaatccgaa
    39001 cattataaat aaagtccgtt aaaatttcga tttattaata aaacaacgtg ttttatcata
    39061 tactaaaaca aaacataagt aaacaaaaaa ttcggatttt aagcaaaatc cgaacgttcc
    39121 atctgattaa atccctttat ctcaaagttc atgaacattc actttggatt acaagaattt
    39181 ctttattgtg ttaataaagt ttcttcattc tagtctcaaa aatcaatagt gtgatttgat
    39241 tttaccattt cgtttctgtt cttctttgat gaattctttt gtatcgacag gattcagatt
    39301 atttagtccg ccaaaacctg tatcgaccat ttttccccag ccttttgtct tcttttttcc
    39361 tgatttgaga ttctttgctc gaagcttctc actatgtttt gctcgccgat acttcaaata
    39421 accttctaat cttcccatta gatccactgt ttttcctcct ttttattcaa tcagacgact
    39481 cagcttttcc tataacaaaa taattgcctt cagggtcaga aaaattgaaa gtcaagatgc
    39541 cattgttatc tgtgatttct cctggttctt ccatgttctc gtagatagtt tcaaattcat
    39601 ccgtaaaaaa tactaaagaa ggcggtggtc ctaatacctc tggtgaaaac ttctgcacaa
    39661 atacttttgg gaatacgcct aattctaatt cttttgagat gctgagtaca atactcttaa
    39721 attcttctgg caattcgatt gtttcagaaa tttctgcacc aattttttct acaaagaatc
    39781 ggcaaatcat ctctacgtca ttgacataaa gcatcacacg tgttttttgt atcatcattt
    39841 gacccttctt tctatttgat tttctttcct gtctataaac tgattccatt atatcacgaa
    39901 agcgcaaaca ccttgtgttt gatactattc aagaaaaata aagcaatagt ttctaggatt
    39961 gctttcctcc acaaactttt tttacaaaaa aaaaacggca atgaatgccg ctcttcttat
    40021 ttcgattctt ttataattct gcttctgatt catctttggt tagtgtgtgt ctaacactag
    40081 ttacgccaaa tgtttgatcg attttttcct tcaacgcttc gattgcctgt gcgccacgtg
    40141 ccgcttgttc agcattgatg gattcaatga ccagcaccgc tgcttgtgtt tcttccgtca
    40201 gcatcgttct ttgggcttca cgccagttga gacagcgaca gatcgctcct tgttcatcat
    40261 agtagatgat ttcttccgga agtgccggag catcggaatc tgcacctaaa ggcaggaatg
    40321 attcgccacc ttgggctttt cctaaatgca gatttcctgc gatcttttcg atatcttctc
    40381 cgccgcaagg tacgccaaaa gacaaggaaa tactattata gatatctaca agcggattga
    40441 ttgggaaaaa ttcccgttct tgatttgcac gtttcagtaa agcttcaata gaagaacgtg
    40501 cacctttttt agtcttgaat gtggtaaatg cctgacgcca ctgagcaatg acttcgtttt
    40561 ggctgaatgt ttcttctgta aggtaatttc ttgcttcttt tgctgcctca ttcaataatt
    40621 gatgatacgt ttcttcactt tcttttgtta cgtggttatc gataccattt acggataaga
    40681 tttcgatggt tgcttcagga aatacttccc agaaattggt atccactatt acttttttca
    40741 tctgttcaca cctcttgatt agaataattg cttgatgaat agatagtctg tttggggatc
    40801 atctcccata taaaaaacat gttcgctgta tttgataaat ccttttttac gataaaaagc
    40861 gagcgcaaca tcgttgtgtt cccaaacacc gagccaaatg gctgtttttt gcttttcttt
    40921 tgctttttca atagctgtat ctagtaaaaa tgtaccgata ccttgatgtt taaatttttt
    40981 tcgtacatag attcgctcga tttctaaaaa atgatcagcc atttcctcag attgactcgt
    41041 tcctgtattt agtttcaggt aacccgcagg ctcttgatcg atactcacta ggaaaaaatc
    41101 ggtttcttta tcttttagct cttctgtaag tttttctagt gcataagctt gtgtcaagta
    41161 atcagctaaa tgctccggtg aattatcttt tccaaatgta tctgtaaatg tttcgatact
    41221 aatttgttgt agtaagtcta catctgaagg cgtcattttt cgaatttcgt atttattcac
    41281 ggcttcttcc tttcaataga tccttttatt gccttttttg acaaaattcc aatcttcgct
    41341 aacattcttg catactttat ttaaaagata ttccaattgt tttgcttctt cgtcagaaag
    41401 accatttaat gcgactgcat tggagtagtt gttttctcga atgatgaaag gatagatttc
    41461 tgctcctttt ttagtcggat agagtttctt gattttttta ttttctttat cttccaaacg
    41521 ttcgatcatc ccattggatt ctaatttctt gattgctcgg gcagtcgttg tacgatctac
    41581 tttgatcatt tctgctagtt tttcttggat gattccagga ttttcgcaaa tacgtactaa
    41641 ataaagatac tgtcctcttg ttaaatcgac ttctttgaat tcgatattgc taatagaatc
    41701 tagcgctcgg gcaatcattc cgatgtctcg aaggatttca gccatttcat cccaccttta
    41761 ttttgtttgt aagactagtt cacctctttt tgttgcaaat gcaactattt tcaataaaaa
    41821 aatcaagttc ttttttaata actagtaaag tatattcttt tcttctttta cgaaaaagct
    41881 caatcacaat agatctggtt tttgttttct aaaataagta actgtttctt cgttgttcgc
    41941 ttttctgata gctttggcaa ctcttctata ctaaaaaatc ccatgttgga agtttcatta
    42001 ttctcaataa acttggcctc ttcaccgtgg atcgcacaag caaaaacaag tttataatac
    42061 tgaaaaagct gtggaatatc tttttgttta ttcgtatcaa aaactgctcg caactgagta
    42121 gtttctactg ttaatcctgt ttcttcatag acttctttac gcacattttc ttcaggagac
    42181 aatcctattt cagcaaatcc accaggaagt gaccattctt tcgtccgttt atcttcgatt
    42241 agtaggacct tgccatcttt tttgatatag gcacgaacat ctacttttgg tgttggatgt
    42301 ccttctccaa tcgttgtgag cgcttttatt tcttcgaaag tttcttttcc tatttcactg
    42361 atcagttcca atgaaatcgt ccggatctcc tcaaaacgct ctttatcaaa tacatctttt
    42421 ccataatata gaccagcttg agaaagtgcg agtagtcttt tgtattgagc caacttcttg
    42481 ttcattctct tcccttcttt tttagcaaat gtatgactat gtaatcaagt atagaagact
    42541 ggtttaatta cggcaaataa atataatgaa atctgcaata aataaaagaa tgttaaaaaa
    42601 ctcgagaaca aaagacattc gacttctctg gggctgtgaa taaatggcgt tcaaaagctt
    42661 gcatctgcgg taataagccc caccaaacga aaaatatgac gagctattct tgtctggctg
    42721 ttcttattaa ttgaggcagg acagcttttt cacaacctcc ccactaaacg gtgttctcct
    42781 accaactcct cggtcacaag tcacaatatt catcaaacat gagaagctgt ttttttgaaa
    42841 ttgctccttg tgactcggag ctgaacgaca ggctcacgac ctctcggtaa acagcgttca
    42901 aaagccaatt tttcgatatt aagccatttc tttgttactt attgtcattg atttcttttt
    42961 ctaatttatc caatagtgga gtgtaatcca cttgagtata gtctgttaca tcaaattcat
    43021 atagtgttcc taactggaat ttgtcatatt ttcggtcggc gatgatcttt ttgaatgctt
    43081 ctttcgtaat atgattggtc cctttcgttc gatcttccaa tacgtcacca tagtgataat
    43141 gatccatgat atagctcaaa tgacgatctg attcacgatc acgtttgtac cgacgttgcc
    43201 ataacacttc aaagtctgct gttaggcgaa tcgtgatcac ctgatatccg tatctatctg
    43261 ctaattcttg gagtcgtgct ttttgcttat cactaaaagg atactcggac acaatcactt
    43321 tctttcctgc ttccatatac aaatcaagtg ctttgtaata gaaaggataa actttcgtcc
    43381 tttcttgttc cgcccgttct tccaaattgt taaacccgat ggattctgca tagtattcct
    43441 tgatttcatc gagcgtgatg gcatacatat ctggaaatcg ttcccgtagt ttattcataa
    43501 gatatgtttt tcctgtacca ggactaccag ccagaagaat gaaatacttt ttcatctcat
    43561 ttatttccta agttaattgg actttccaag ttcataaaca gtccaatctt atgcttgaca
    43621 atttctttta ctgcttcatt tgctggaatc acattatggc gaagagactt gttgatttct
    43681 gtttcactaa aggtttcttt agctactttc gtccagttga ctaaaagctc cgttccaaca
    43741 ttgaatttgc ggatcccgtt attgatcaat tcaggataat cttcttcttt cacacctgtt
    43801 cctccatgga taactagggg gacatcaact actgcatcga tttctttcag caagggaatg
    43861 tttacttctg tttttgattt gaattgtcca tgattcgtac cgatagcaat agctaatgca
    43921 tccacgcctg tagcttctac aaatttcact gcgtcttccg gtcttgtata taccttgtca
    43981 ttttcagcga catggattcc ttcttctgta ccgccgattg ttccaagttc tccttctacg
    44041 gaaacaccgt gcgcatgggc atattctact actgcttttg tcttcaggat attttctttg
    44101 aaaggcagat gggacccatc atacatgaca gaagtatatc cgctgtcgat tgcttctttg
    44161 atatcatcaa agtctcgggc gtggtctaag tgcaagactg catcaaccat ttcttcttct
    44221 gcaatagctg tcattacatt gactaacaca tgcatgccga tatatttagc ggtatcgaca
    44281 cttgtttgaa taatgatcgg cgaacccatt tcttttgctg cgcctagcat agcaggaagc
    44341 atttctaggt tatgcgtatt aaaagctcca actgtaaaat tcaactcttc tgctgttttt
    44401 gttacttctt tcaatgttgt atacatcttt tattgccacc ttatcttttt atttgtttcc
    44461 tttgatcgtt acttgttttg caatttgtcg catctcatcc attttgccca tgtagtaatc
    44521 gatcccttga tcatcaccag cacgagcagc atctgcacgc ttctggttat agaggctcat
    44581 cagtttttta tacccatctc cgatcgtctg tttattttcc aaactctttt ctaaatattg
    44641 gatggcttga tcgttcacat acaattcaga aaatttaagt ccaacttgtt cataaagttt
    44701 cgctaattta tgcttatctt gggtagtgga aatctcttgt ttcaattgtg taatctctga
    44761 ttgaatcttt tcggtcgctt ctggcgatag ggctttttct tcttttgcca gaactttttt
    44821 cggttctttt tcttttttct taaaaaatcc aaacatcatt ttccctactt tcaattaaaa
    44881 tgctttcaaa actgggctta agatccaggc atataagagt gctgcggcta ctgtatctac
    44941 atcggttgct gtagcattgt tgaatcccat cgaattaaag atcgttacta gcaaagcggg
    45001 caataatgta ataaagaaac catgagcaat accgccaatg atcgctcctc ggcgtccgcc
    45061 aactgcgttg ccaaatatcc ctgctgtccc tccggcaaag aaattggtta gcataccagg
    45121 taaaatcatc gctaagccga acatgggcaa agtaaacatg gcaataatcg taccaatcgt
    45181 tgtcgtaatg aaacctaaaa tgacagcatt tgggctataa ggaaagaaaa ctggacaatc
    45241 taaggcaggg atcgcatcag gaactaattt catagctatc cctcgaaatg ccggcacgat
    45301 ttcacctaac agtagacgta caccagctag caatacatac acacccacta cgaattggat
    45361 ggcttgtaaa aaggcaaaca tgatatagtt ttgatctcct gataattcag aagcgaatgg
    45421 ttcgcctgca aataaaacag tgatgatata tagcgggacc atcacgacca tgacagacaa
    45481 ataagtatct tgcagaaatt cgaaatgtgt cggcaacttg atatcttcga tcgacttttt
    45541 attttcgcct ttctcaccaa aaagcttagc aacaccggct tcaaataagt acccgatcgt
    45601 acaaaaatga cctaaagcaa tatcatttga tccagtgatt ttacgaatga tcggttgcgc
    45661 tactgccggc atagcaatgg cgaaacaagc tccgataaaa ccaccgacca agatcaatac
    45721 gatccctcgc agaccggcaa aatagccaaa gactgtcgtc atcgttgcca tccataaaat
    45781 tgcttgacca gttaaaaaaa tgtacttcca ttttgtgaaa cgagccaaaa taatattgaa
    45841 tacaaaaatg gctaagaacg tcagtgcaat atctctacct aaacccaaat cattcattgc
    45901 ttgaccattg atagattcaa tagaagggat gatcccttgc atatgaaatc cttcagtaaa
    45961 gattttcccg aaataagtta aacttccaac gatgatgctc gaaccggcac tcaacacttg
    46021 aaatcctagg agtgtcttta acgtgccaga gatgacttgt cctgctgatt tcttctgcag
    46081 tagtagaccc aacatagcaa tcaatgcaat ggtgatcgat gcctgagtca aaatattttc
    46141 gatgataaaa ttgataacgc ccatgttttg atgtgcaccc caaaagttag actagaaatc
    46201 taattaaaag gggtgctttt tttgcggaaa tatacatttg tttttaagaa aaaagtagtt
    46261 tcagactatt taaataacga aggcggctac aaatatcttg cacataaata tcaaataaat
    46321 cgtacactgg ttagacattg ggtaaggatt tataactatc atggttggga aggcttggtt
    46381 ggaggtggca aaagctacac tacaaaattt aaacttgatg ttatagaata tatggaaaca
    46441 aatggtcttt ctatccaaga aactgctaaa aaatttaata tcggttcaaa tagaactcta
    46501 agtaaatgga tagagcaata tgaagaaggc ggtgcttctt cacttgagag ccaaaaaagg
    46561 ggcagaaaaa ttagtatgaa ttccaagcta aacattccta aaaaacttaa agatgagtct
    46621 cttgaagaag aagttattcg tttaagagca gagaacgcat atttaaaaaa gttagaaacc
    46681 ttgattcaag aacaagattt gagcaagaag aaatcaaggt taaaacaatc cttcagctca
    46741 aaaaagaatt taaattgaat ctattacttt caattgcaca attagcgaag tcaacttatt
    46801 attactgggt aaaaaaatta gataagccag ataaatatag caagataaag caagaaatta
    46861 cagcgattgt aaaagaatca agaaactctt atggttatcg tagagtcact ttagcgttaa
    46921 agatgaaagg atatacaatc aatcataaaa cagttagaaa attaatgtcc caaatgggac
    46981 ttacctgtca aatccgaata aagcgatata aatcttataa aggaacagta ggaaaaattg
    47041 ccaagaatgt gttaaaacga aatttttcag tagatacacc caataaaaaa tgggtgacag
    47101 acgtcactga attcaagata aaaggaagaa aaatctatct atctcctata cttgatttat
    47161 ttaacggaga aataattagt tatagtatat caactagtcc aacatataaa ctgattgaag
    47221 aaatgcttca acaagcaatt aaaaaaaagg gaactgaggg ttcactgatt ctacattcag
    47281 atcaaggatg gcagtatcag atgccacaat atcaaaaaaa attaaaagag aataacatta
    47341 ttcaaagcat gtctagaaaa ggaaattgct tagataattc tgtaatagaa aatttttttg
    47401 gtgtgcttaa atcagagttt ttttatcgag aaaaattccg atcaattgag atatttcaaa
    47461 gtaaattaaa tgagtatatt aggtggtata ataacaaaag gataaaacta aagttaaatg
    47521 gactatctcc agtagaatat cggaaacagt ccattaaata gtt
//
LOCUS       DAZKLM010000010        46206 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3764
            SAMEA115706913-rid22582563.denovo.010, whole genome shotgun
            sequence.
ACCESSION   DAZKLM010000010 DAZKLM010000000
VERSION     DAZKLM010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706913
            Sequence Read Archive: ERR13196647
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 46206)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 46206)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287823.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287823.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,004
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,849
            CDSs (with protein)               :: 2,849
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 41 of 96
            Pseudo Genes (incomplete)         :: 52 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 15 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46206
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706913-rid22582563.denovo.010"
                     /isolate="VRE3764"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..132)
                     /locus_tag="ACG842_000511"
     CDS             complement(<1..132)
                     /locus_tag="ACG842_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HFQ0247075.1"
                     /translation="MKLFVGIDVSSEKLDVCFLTDDNQLSILSEISVANDIEGASFIR
                     "
     gene            542..781
                     /locus_tag="ACG842_000512"
     CDS             542..781
                     /locus_tag="ACG842_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0247076.1"
                     /translation="MARPVLEAMDMDTLTKGRYVQLLARGYSPKEVFKALSKGTDMEK
                     ERLRKEFDYWRTHNGIKDLKPARPVIKRKKKITQD"
     gene            848..1057
                     /locus_tag="ACG842_000513"
     CDS             848..1057
                     /locus_tag="ACG842_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0247077.1"
                     /translation="MNYEEIENRKKVSKEMEEKLLKTMKQKHLKRLSVAQYINDMQLT
                     GKEKACLLGSMKNFEQLRRTYRIHF"
     gene            1117..1788
                     /locus_tag="ACG842_000514"
     CDS             1117..1788
                     /locus_tag="ACG842_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HFQ0247078.1"
                     /translation="MKKWLFGFLGVTLIVFCSVFGYVSYQKHEGEVFKQNIEKKMPVD
                     QINAHAKSYKEDATNVNNDMSLGQMLSIQKEAIEMGVNKQVFAQIQIPALGLALPIFK
                     GANQYTLSLGAATYFYEDAEMGKGNYVLAGHNMEMPGVLFSDIQKLSLGEVMDLVSND
                     GVYRYKVTRKFIVPGYFKLIDGVPEENSFLSLPKKGEKPLLTLFTCVYTSQGKERYVV
                     QGELQ"
     gene            1841..3817
                     /locus_tag="ACG842_000515"
     CDS             1841..3817
                     /locus_tag="ACG842_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HFQ0247079.1"
                     /translation="MAINRYVKKAGMLVGMLGIIAGCFQGYQVHAEDVTQKTPPEKVN
                     ITVHKLMYDQGTQLNVDIDGIKNDGYTHDQYPTGVTKYNKADYGDVEFTLGNITDQVL
                     PREDSDLTNAKVDEIVQDVEDKGSNSEYVKNATNKITSAVDENGEITFTDQPAYVNSK
                     GNVYVVYESKSAAGLVTQKAKPMVVIAPMTDKTGSGFLKNIHLYPKNIVSKLSFELTK
                     FGDDGADQSKQTLLKGAKFELYKGEPGKGTKLGDLVSDDQGKLTATDLTLGKYYFVEV
                     PSEVVVGSDQEPTADQYLLGADARNDAHNKLTFEITNDGVTSDLKASYVNYKAPVIDK
                     TVTNGTGQEHSFQIGDAVNYQGTIHIPTDIAGGADGITVNGVKSETSPYSVFKWGDTA
                     GKGLSYVAEKADIKVTNNDGSVVLKENTDYKIENSDSGFVIDFIVNNGQVSDTVANLH
                     GQDLKMTYNMFVNDSAEIANPLTNSVDFVYNNNPFNQEEHHEKTKADVVTYGAKFLKV
                     DSGLFGTGIKATPLEGAEFAAKNAEGKYYGGLTDTDKDGVKEAVWVDDVANAAILKSD
                     KEGHFEITGLTEGDYSLEETKAPENYQKLTKEISFKVDKDSYKEENRITIKNNQKASV
                     PMTGSNGFQSYVLISCLLLGAGGLSAVVYFKKKA"
     gene            3830..4585
                     /locus_tag="ACG842_000516"
     CDS             3830..4585
                     /locus_tag="ACG842_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFQ0247080.1"
                     /translation="MIIRKPPKRRVMHKLMIFFWLLSAVAVYLIPVVETNVAKFQQQQ
                     RILAIENLEKTDSSLDVAKIELGDPVGVLTIPSISLKLPIYDGTSDKILENGVGITEG
                     TGDITGGNGKNPLIAGHSGLYKDNLFDDLPSVKKGQKFFIKVNEEQHAYQIYRIEEVK
                     AEELQKNYLDYLMPEKDQDRITLMTCTPKGVNSHRFLVYGERVSFSKEDLKLQKKAKN
                     RKSISLDWRLALLFAFFCVPIIFIYKKIRRRSE"
     gene            4582..5406
                     /locus_tag="ACG842_000517"
     CDS             4582..5406
                     /locus_tag="ACG842_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilin N-terminal domain-containing protein"
                     /protein_id="HFQ0247081.1"
                     /translation="MNKRKWLFVCVAALFSTVAYSQLSFADSSDSSTTQTIDNSITAP
                     SETDSSENTTEETADSNISFTESTNPTSESSSETSEKPQDVEDTQVIKQAIHIQKIIS
                     SNQINNQGSQVAQQEGINGAKFIVYDITNILEEMSQDDNEKNQSVEIIESKLKDRAKK
                     LSYDQLKKVAEGETKTIDDQEGVIEFSIEVPANKKQAYYIVNESSPENISNSEDIVLL
                     TPVSNEKGEFLTDVWLYPKSEKSEPKEEIKKVVSTGVKKNFFENCWEFFAQLFFDN"
     gene            5416..5664
                     /locus_tag="ACG842_000518"
     CDS             5416..5664
                     /locus_tag="ACG842_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0247082.1"
                     /translation="MKIIRILVFNLSFLAATILCCGLIFSQLSIYPRYQRLFIHFLNI
                     QCASMPLEVIKKLLINPIFMVSLVLLTGLVTWVLGKKE"
     gene            5707..7806
                     /locus_tag="ACG842_000519"
     CDS             5707..7806
                     /locus_tag="ACG842_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HFQ0247083.1"
                     /translation="MENKIHCTTGRVITLVLFLFLLLFARGTAVQALEKVDPSQIKEN
                     NNLVLYHDSDSLRNIEVTGINGFKILDEYSTKKQKVLAWPKVKDLKSTSKIVAHYQKV
                     GTYLGKEIDCDVTFSNFVAHAGVNQDGLAKTINGKKYYNYLLFDKNIYRGFFMQSINV
                     MNAKLEFKYAGTKTKVALSEPQPEIQSKMRSMRAAGIDLNDTFLSIYSLNGQYQQRVS
                     WYNDNNESVGERNIDMREFVGYEKMGSLPYFVTTDTACAEYKAPALSTNNLTSKVVGG
                     RAEKAYSSNDHFTDVVGADTYHRSGVTYTLQGASPTFMIGANTNSMMFSFDTALLWTP
                     QPSKPTKEVFNKANTEEAAHNIDKKVIPQGSDVYYHIHQKFDALTVNTMNKYKSFKIT
                     DTFDSKNFDMVSDGKNYDGALWYVEANGTWKKADTGGKLTVSGNTVTYTASDSFLANA
                     ANYGRDYILQLHMRAKNDAKGIAPNVATVTFNNDPNKGTQKTNTVENYFSLKPNKKVE
                     QDGKDVDGRNDGKKGTPTAPLNAGSEVQYLVTQKWHTKGVDIASDHYKQFSIQDPVED
                     RLTYKEGSARVIDMSTGKDITSEGTLTYDSNSRTLKWEASADFLSKNLLDGREIQLIF
                     TAKTPLNEELDIDNQAKVSVESIEKATNVVTIGVKPNLPQIIVPKTGSNHLVLTVIGS
                     ILFLTLASLGYVVLKLK"
     gene            complement(7844..9901)
                     /locus_tag="ACG842_000520"
     CDS             complement(7844..9901)
                     /locus_tag="ACG842_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HFQ0247084.1"
                     /translation="MKLFNPKTVLAIGTFAASLSLAGSVDAASTNVMYRLYNPNNNEH
                     FYTSSKKERDHLVKIKWGNYEGPAWEAPTSGGHLVYRLYNKGLRDHHYTASWDEVKWL
                     TKNYGWTYEGPAWRSAEKNNKPIYRLFLPGVKSGSHHYTASWKEVQWLTKDYGWKYEG
                     IGWYGADTSKPKVNKSALEALYTSVKGTKKGDYTDNTWNAFQTALNNAKKVLNDSKAT
                     QKQVDSAKNKLDSAYKGLKKKPVPTVNKAELQALYNRVKSTAKGDFTEESWNNFQTAL
                     SNAKKVLDDSKVSQTQVNNAKNSLDAAFKGLQHKPETPKEYTVTINHIDNQTGQVIAT
                     DQAKVTSGSTYTAKAKAFKYSEGAKDNFSYKVIGNDTQSKTITGNTTITFNYNQVHQI
                     NLLCNNNYKNEGRVNILKEQIVNVTHGDSVTLTAPSIQGYVLDDRVEPTNSVTLNNVT
                     ESQNYIFNYTRKFNVTVNHVNADTNAVLSSESKTVYEGDSFATPWKNMTDQNYFLCRN
                     DDPTVTVDKNGQRSINNVDQNKTITFKYKKISLDELRNFMRQKELAWLNDYRQQNGVA
                     PMQFNDIVQQAADIRAKELQVSFSHYRPGGGTFQDLLESLGCYGAKGENISQTGLYID
                     DLLSDDGAIDAMVGWKNSPAHNANLLYNNQSILGLGHNFVVDSAGRIGVDNVFISANP
                     IFK"
     gene            complement(10015..10227)
                     /locus_tag="ACG842_000521"
     CDS             complement(10015..10227)
                     /locus_tag="ACG842_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0247085.1"
                     /translation="MYADKKKIEWLLFDSGKSVRSISIESGVPLTTVSDLANKRSSID
                     NMRFINAMKLNKLAEEIVDINTSTNK"
     gene            10414..10881
                     /locus_tag="ACG842_000522"
     CDS             10414..10881
                     /locus_tag="ACG842_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0247086.1"
                     /translation="MKKKQLWFVSMFAIFFIILSGCSSNKKSEAKEDNSLLQPGTVWK
                     EEVGGLIHNLKIIDDTTWEYSESVWHPDPVQITVKRQKDYEGLERYKIVDSAGIREFI
                     NKPDSLFIVVPYEKDGVKKIIFLGSSKDEKRTKEKLIHDGSELNKYKLQKTSE"
     gene            10878..11120
                     /locus_tag="ACG842_000523"
     CDS             10878..11120
                     /locus_tag="ACG842_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5415 family protein"
                     /protein_id="HFQ0247087.1"
                     /translation="MIASLFIYPEGGITVAKVKTPFEKAASKILERRGVSEIQWKKEV
                     LEKAQFDLLRGKDKDLEEYIYQREREKLVFDEIIKH"
     gene            11138..11440
                     /locus_tag="ACG842_000524"
     CDS             11138..11440
                     /locus_tag="ACG842_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0247088.1"
                     /translation="MFESITMNFLTQPKVVAAVDIFRALQTAATSLKNNLMIFAAVAA
                     VASIALGGIFFFFGRRGAENGKTHVGYVLLGVGVILAAASIVVSMFSMFGQTPQGL"
     gene            11512..13566
                     /locus_tag="ACG842_000525"
     CDS             11512..13566
                     /locus_tag="ACG842_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0247089.1"
                     /translation="MFFTISGNAKLDKELIRKATKKVGKRTSFLPKTIKYSVIETEEQ
                     LHVLGQVLEQENQSSVKLTLTLHDKEGKIEKAGTEYVFGPLDYQISEGFYPTLIDLIR
                     LDVFKDTDETMSVEESYRNEKLLQVLEEELESDQRKVNQVSEVEVNEEVPWERPIFPK
                     KESKEKETVYVDEQADIPTQKSIFEEENDVMGQLEVDPFEETMEDEETDEVEVDPVEA
                     NEESTWTEGDFQEYIEPLISEEVEKNETEIQKATVFDYILDQYPPEHEWMKEKNKLFL
                     NELYSKYQLPQTYQAFLESKEELISQGKGTLLEQLESVQSTNWHELAQEDLLATFEER
                     QQETDQEINEYATEQSSNWEKVKEELNKEEQRVIEEEVQKVKERYEKKRDNAYSLCVE
                     KINNFTKTNQEALSKEKENLLKERIKERKEEFYRGLRTDKLKISKELNEQLNKLYQTT
                     TEVLMEKHEQIQQELQVQIPKWKKEYEIQQQELKEQEEKQRQKEKEREQLEIRKREQD
                     LAEQKMAIEKQRLEQEKEKEAAARKERQEQMDTLRQAQLNYLAQPQPVAAVQTPVNNQ
                     ANLTPSPQRSFNGWMVGCIASLSLLLGGGTVFAFNHINQSSQADSVATTASIKEEAQE
                     QARKEYEQQLEELLNRTNQSTTESSMENSKESDPSKAKEGSSDSTVHSNTSESSESNE
                     VK"
     gene            13566..16178
                     /locus_tag="ACG842_000526"
     CDS             13566..16178
                     /locus_tag="ACG842_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirD4-like conjugal transfer protein, CD1115
                     family"
                     /protein_id="HFQ0247090.1"
                     /translation="MRIRETTRRKDKRFNLEKNRYLSHFYRYTPSGEELPEKHDKRIS
                     QKIFYSGLAYLPLNYVVTRIPEAFHRFEIIPGSLQGVNIFQLQEMPQLFHFDSTGFWN
                     PLTPTTSLGTRLVVSTICVVPAVLMGLKMDMWFRPLADGQKGDNRLMTLKEIQQIYPQ
                     IPETKTTFPGYGGIPITHYKKYWYIQTDTVNTCIVGTSRSGKGQIEVLATIDNLSRAE
                     KQSSMVVNDPKTELYVGSKDELEARGYDVYAFNITDPLQSMSDNPLALIVKYWKRGDI
                     DTATQLTNTFTNTIYYDANASDNKYFNENAQKAVNALIFALLEQADQTGDYSKLTPNN
                     LVELLSEIGGFNYYPDPDNEFKQLNALDEFMNQLPPGNIAKKQYGATKIGSDKAKGNI
                     LSTAITGLNPFTLTKIAKMTSQSTIDYKSVGFPKYLDLKLHESLLNQRVVVEFYHKGK
                     KFHEERVKVGYKGFSEINFDCHLVAGDQVCLKYDHQGKEYVAWYEFSQRVLKDEEGNI
                     VYRKKRGETHLPELEKEAVLTLDEEKSNLLVESIRMHYTDKPMAIFMLTPDYDPSNHI
                     IVSIFLSQLYKELSTQCVKTKGDKCHRRIHFILDEFGNMPPMDNMEGIMTVTAGRNML
                     WDLFIQSYQQLYAKYGEQNAAIIKENCQTHIYIMSTDDNTIEEFSKKVGNKTVEQENS
                     TINSLGMNTHINRNVDSDRILTPERISTFLEGETIVLAPLKRQDLKGHKIRPYPIFNH
                     GETNLPYAYQYIEDFNPSWDINDIDIHCTHADLDLKALQIDYDYFLPDEYARNLYHGL
                     HDQLEEKNEENMFYKIVQSFENETAKKLLIQFYEEQAADRLQEYVEKLIKNKEISEMI
                     GKRLLLESEKVVKE"
     gene            16175..18706
                     /locus_tag="ACG842_000527"
     CDS             16175..18706
                     /locus_tag="ACG842_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pLS20_p028 family conjugation system
                     transmembrane protein"
                     /protein_id="HFQ0247091.1"
                     /translation="MIIGGIFSGIADWWSGLFGEGDANPKTVTKVLQDYGNYLQYQDV
                     FSWIWNTFLWGLIKGLYNLNTLLEKTIYQSFDLKDLLNAAGVNALFQSLISKVLALFC
                     VVTLIYLGLKFFMSKHPPKVKNILVNLFMAMILIFGGSSLIDEGLNISKSFYGDVTAA
                     NKADKTGSPAFQIIQNNVIDVSTLLATDPNKVESLPTDKRNALTAKNFKTANINGIIT
                     PEQAKGMAGNDKSGLSDIQKDRLKALQYRLELDDNGKEIPVQISDEGLAKYVYTSGYR
                     RYSSKPGIILAGESSLAVAYLFILFTIVTCIIELVFKKFYLVIAASTDFETGQRMKSA
                     IEDISQSFLLLAFTILELRIYTLMLSGIGDLHAAGKINGFLYVVALIVLTIALFKGSQ
                     SVTKIFGVDTSLKNGSNSLLSMFALGNIAKNVGSGAKNLASAGKSGLSHLNDIRKNGF
                     GHRKDVTPDNGEEQSDDKATTVPNSKKTVSEMFGKAKNGVSKAAEGLGYVNERGLKGT
                     AEDLTEKAKDTLEDKLDGTRAKELAEAVKNPSEALEKQKEKASEVKEGLKDAYGEGQV
                     NAAVKANTTTEKENQEGIVIPSVKDHQEALHSNENSIEEKNSIEERNSEEGKNGQLPK
                     TDPEQYRYKPNSDLENEKRISDLSKVNQEDKAENEGQLPKTDPEQYRYRSVNSENEKR
                     ISDLPKRNQEVQTDEDSIVLPNDNGIEVGTEKSTKIGLPKAQNLPMKDSRLNQFKDND
                     QSFVHQSPAGTVQMKPSRLPENGNLTTVSYNLSESGETGNASVQNVVTREAAPKPIQN
                     VHTGYKPANVAFTAPTKINVTRETKNRNNALKEQLLRDIKQDIKP"
     gene            18758..19090
                     /locus_tag="ACG842_000528"
     CDS             18758..19090
                     /locus_tag="ACG842_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0247092.1"
                     /translation="MDKYSNMKNPPRFSGEWKFFAFFFLDLLTLGGCFFWAKMVNNLV
                     HLPLFFALLNYGLWIAIALVIVWRPYNNPGQRQIFAIIESIFFADKNHYHALDPNRNY
                     HGTYKEDE"
     gene            19091..19684
                     /locus_tag="ACG842_000529"
     CDS             19091..19684
                     /locus_tag="ACG842_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0247093.1"
                     /translation="MRVKGQNSVERKSILQFGKPEERSILDLFPYKSCTEEGVLITRE
                     DHFQRYYRVVSTDVEGLNEQEKLERMNQLTTVMRTYVPTIKLVSLTTETDLTEQIVQK
                     RQLLDKNRMEQSLGRNLQRLKKYEKKLVEEIQELKRAEQERPDLSFFFVIEGKTIKDL
                     QTRNRQLMRSSGILGLKPLYKKELIKILYRMNNMNDE"
     gene            19847..21541
                     /locus_tag="ACG842_000530"
     CDS             19847..21541
                     /locus_tag="ACG842_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HFQ0247094.1"
                     /translation="MLEAIKTAFSPQNDQKVLDPLKQLGNREIEIVKSKGYDLDFLKQ
                     VMPQGGLNFDEEYVTYGNGYSRCVTVYHYTKNPTLFWLANLMNNPHTVATLDVSTDEK
                     QKVIDQINRALDELNDRSDNERKATDRNKSAEEYLELAEYARKLTSNGEISKQVKVRV
                     HVYGNTIQEVDERAQELIENLKGMDYRAVTYLHKTKRDWQSMFTMFDEQAEWFGNIKG
                     QSLPSLNIGGGFPFHHQTLKDPGGTQYGETMTGGPFVWNATYKNSVRLSTNTLLLGMM
                     GAGKSTVLKMIAEAHLAAGDFIWGFEKGKDFIPFLKEYNGIMVRLDGSDGMINPLEIF
                     ATRTYDEASSLYDDGSVKDLKINEAASYQTHLDKVVYQVQLVSPQLKGTMKAEFKTYL
                     NRFYEEYGTVPRGFTSSNSRSNTDTQVTGKDPEAYPTFKEFLDFLGQLELPGASQEKK
                     NRKEEMESIVESLCETYGMIFDGHSTIRHLDQQQLVFFDIDSITGLDRGVQQCQMYMA
                     MTLIWNQALIQGRRQRRLIKEKQMEEQDFKRFLAMIDECHNGATCSAVKSCGLQVV"
     gene            22229..24106
                     /gene="ltrA"
                     /locus_tag="ACG842_000531"
     CDS             22229..24106
                     /gene="ltrA"
                     /locus_tag="ACG842_000531"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HFQ0247095.1"
                     /translation="MTKETSPKDRKLRHAEYYGMTEIFDKIYSKSLKGDNFKNLMQII
                     ISDNNIVLAYRNIKRNHGSVTRGEDGVTIKDIENLNRNEFVEIVRKRFAHYNPRKVRR
                     VEIPKYNGKLRPLGIPSIWDRIAQQCILQVLEPICEAKFNNHSYGFRPNRSAEHAIAD
                     CMARMNQSHMEYVVDVDIKGFFDEVNHTKLMRQIWSMGIRDKQLLLIIRKMLRAPVVL
                     PNGKLIYPTKGTPQGGILSPLLANINLNEFDWWIANQWEEKDAKELKHHFRKNGAWDK
                     GNIYHKLRKSTTLKEIYLVRYADDFKIFCRNYNEAKKVFYATKMWLQERLKLPISEEK
                     SKITNLSKEESEFLGFTMKLVTKGNKKVVNTHIGTKAQSMIKVKLKKQIKRIQKSPNS
                     KDTFIYIGVYNSMVIGIHNYFKIATHCSVAMRKIARELRPSFEIRLREQGISKEGQYG
                     KYEGYKPYLRSGQIRYIRGFPILPVGYIQHRHPMCKKRNINKYTEEGREAIHKKQQTV
                     EEWKISWLCSNPVLSKRATIEFADNRISKFIAQKGRCAVTGEELILSEMHCHHIIPYH
                     ESKSDSYENLVIVTEEVHRVIHATQSETIEELLKYLKLNPKQKEKLNELRLKVGNEEI
                     S"
     gene            24237..24614
                     /locus_tag="ACG842_000532"
                     /pseudo
     CDS             24237..24614
                     /locus_tag="ACG842_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307536.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
     gene            24614..25819
                     /locus_tag="ACG842_000533"
     CDS             24614..25819
                     /locus_tag="ACG842_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HFQ0247096.1"
                     /translation="MENQNESLIKQYVKRRAKRRLFLWLFGTSAGLITILITVFVTLF
                     LILAAGSIDNSDSDSSSGGEAFTGEYSEGLPIYKEIKGRGPFSDEIAQYAVGAAVKYK
                     LLPSVILSQYGYESAFGTSASARNDLNYFGITWFDGCLFPKGTARGIGGIEGGWYMKF
                     PNSKAAFSYYGFMVATQSNFNACVGNKSPGASLLILGRGGYAAAGITEDSPYYTGCMS
                     IITSNKLTEYDEFAIKHWGEGGNNNGTITGEWTNPFPGSSLDKSSFSGGQLFGTNPGG
                     EFRPNGFHDGLDFGSVDHPGSEIHAVHGGKVVYVGNPGISGLGACVIVINYDGLNMVY
                     QEFANSTGNSRVKVGDQVKVGQVIGIRDTAHLHLGFTRMDWRQAQGHAFIDDGTWIDP
                     LPFLNSSKK"
     gene            25835..26479
                     /locus_tag="ACG842_000534"
     CDS             25835..26479
                     /locus_tag="ACG842_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0247097.1"
                     /translation="MENKTLKIVLTAAISVSLIGNIALFWQLKEEQAANTYKIEEVAK
                     NASEKLKKNSSEAQAKIDKLNQTINEKNQEIDQLKQQDGTKAKEEVTEAQRKTAEEFG
                     QLAIDKSLSRNELTEKLKDVATQEVIDKLSPTDDDHQHDDYGSYSFTLGDVQTYAQTS
                     SVKEEQNFVVFVDYTIGNPQFKDVKPQKIKGGLTVTEKQVDGQWKVIDFSYFTR"
     gene            26490..27296
                     /locus_tag="ACG842_000535"
     CDS             26490..27296
                     /locus_tag="ACG842_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0247098.1"
                     /translation="MSKKKGERQRFKKRKSYLRWLGIFMILGFLILASPIYTNQKVDI
                     PDEVSTNAFLNTKELTMVSKEKGANQQLVFEFAIDDEDQSLLKELANLNYRVEVKTAK
                     GDYQAIRAKVIKVSDDYFAVKLTHVPEEYIAMRLTIIPEKIDPKVDMQEPQDLIFYIH
                     EDKVKDHVKDEDYEQHAINYKVKGYKKEQKAAQKQMESFQATIDLNEELVKKLKDQLP
                     YQVADDQENTENKINSYQQEIETSKTQLKDQEEIIQKLQEKIDRMTKEKI"
     gene            27318..27548
                     /locus_tag="ACG842_000536"
     CDS             27318..27548
                     /locus_tag="ACG842_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0247099.1"
                     /translation="MKRTNLADKDTFAEQKVLIKAYLKVVKAKEICREVNLSNTTISE
                     LKNGRKNLDTLKFRSFEALYVAAKKYFTADNK"
     gene            28094..28369
                     /locus_tag="ACG842_000537"
     CDS             28094..28369
                     /locus_tag="ACG842_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0247100.1"
                     /translation="MDLLIRNIEEKYINKIDKRCEELTVKTGKKWSRNQYLKVLIEND
                     FDHALLNYKKDQFDRLLEKFVDIQTYNTKTLEEYIATNNQLIGLLIE"
     gene            28411..28833
                     /locus_tag="ACG842_000538"
     CDS             28411..28833
                     /locus_tag="ACG842_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0247101.1"
                     /translation="MAKATRKHVYLYDMHIEVLDEIISTHEDISNYSEAVRHLCMMYD
                     PKIEEKINPIQIKLNAMGKEISILTEMVSEFADLHLKDTGILIGTNSTVYQEAEERVE
                     GKIKSNQTKKYSSKLKKPVNESVHTRVDPFDPTKILKR"
     gene            28852..30444
                     /gene="mobP2"
                     /locus_tag="ACG842_000539"
     CDS             28852..30444
                     /gene="mobP2"
                     /locus_tag="ACG842_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobP2 family relaxase"
                     /protein_id="HFQ0247102.1"
                     /translation="MNKSPAVILTCQFTLPNKKSFSTYIDYMTREKALEEMERRTPEE
                     EQELQRIKNALEDFYIPEGETFSESKTDKEFSETSNEAQSLMEHGINFDKLEEQDFTK
                     YLSYMTRHYALEQKKELTQREQKEYQRLNQAMKKYQTEPHKKASGNLPGVFSMASDEV
                     KGSDLKEIKQIFQRGQQKGSIIYQDVISHDNSYLEKMGLYDPKTDTLDEEGLKAAGRK
                     MMETLFEKEQLNETGYWMATIHRNTKHIHIHFAVVEKENTRKPYTEIENGVSYIVPKG
                     KRRQATLDAMKTNYVHELERFSYEKDRMIGMEKRNLLTRKSDLRNTLTKVVKEPARYD
                     RHAMNLLNEVYKTLPEKRSEWNYGDENRTKLSPFTRGKLDELTKYVLTKEPGYQEYIQ
                     ISKALKEEAKSLYGESKRETKNAEKNALFDLKKRTGNAILTELKRQDSQIKPLIESVN
                     QMQSIYNERSFRTLKQLPTNEYKYSYQEFVKRRKQFQQRVVTQRSLRQLNRTINAHEE
                     KYHASKAYEEQQRRIQMEQERQ"
     gene            30456..30791
                     /locus_tag="ACG842_000540"
     CDS             30456..30791
                     /locus_tag="ACG842_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0247103.1"
                     /translation="MKQYLDQWKVIEGSLKEERIEQLPDCLEKEHLFQISEMLRNEQF
                     DPNQFLVVEYPATGVYCCNHVKGEKYFIIQEYEGKLAPYYTTWEINEEGINNFPCESI
                     EESISLTEC"
     gene            30938..31132
                     /locus_tag="ACG842_000541"
     CDS             30938..31132
                     /locus_tag="ACG842_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0247104.1"
                     /translation="MTQTTIGVVVEKSRDNLVFVTEIQTGRAFVVTDKAAKAYQNGNI
                     LTLNLTTKMFVDAVENYPFV"
     gene            31164..33536
                     /locus_tag="ACG842_000542"
     CDS             31164..33536
                     /locus_tag="ACG842_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArdC-like ssDNA-binding domain-containing
                     protein"
                     /protein_id="HFQ0247105.1"
                     /translation="MAEEKEPLDNSRLGKSKRKLVRLQNELNNQFQKLFDHQKLTNGQ
                     PMNDKRNGRSWFNQQEKIENKIQSLREEIKQQEKQVEKLERQEELKEMGYNKYGGLDM
                     TIDNIPRIKEEIERFEKGESTFSAATIRKYQRKLETLEQLKEKSEKGKENILPEVQAI
                     IDSGRVTQWKKNPTIYFLKGYRKVALELNENGGFEESKKYQATSEEEKAAVRELLEEA
                     APTDSLKEKEKDHFYRVEFNENDPEKGLKSYGGEIVTLELIDQLKTLDNQYAAEAGYY
                     KFYFEEVKNGEVVDQIRMDLGDGVNSNHPIYLELAKLCEQVPEKEQKQSVKNEPVSKE
                     QMQSKETKTKQKGYQKRTAEEIKQEVKSLSQQALDAVKKHTHSSEDVKELLNFMSRFP
                     ERSFRNQLLIEEQFPGATACLGRAALKKEGIYIKKGEQGNKIFVRKTVKGFYEKGRGF
                     VRETEATPEDKKRIESGQIEVIKKPYYTIEKVFDITQTQLKPEDYPKIFPNRVFDFQL
                     DKKGQSELQAGIQAVANNIGIQIRDMTESEVYQRELGQARGAYVRNEFTGEEEIVMNT
                     RNTPTQHLATSIHELAHARMHKFSELDTATKELQAEMTSYIVCKHFGMDTSEKAIPYI
                     AAWTKNGQTLDDKEAAERGKIMNDVSRVANEFIQTISNEINQYREREPEIQPAEVKQE
                     HKNTVETAENKNIVPVGSLWIDKKDGKVMEEDTGRTLHLKDAAFIEDPQHNQVILKGK
                     SYGKEIQTALPSEEFALGNPFRFSLETVSEVAKRKNEIYYEQQLSQKERV"
     gene            33597..35057
                     /locus_tag="ACG842_000543"
     CDS             33597..35057
                     /locus_tag="ACG842_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014401506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HFQ0247106.1"
                     /translation="MTEKKMSLIDRCKQIDIVDFARNNGMAVVNKGRDYRLEDHDSFV
                     FDRRKQRFYWNSQNISGDIIELAKLFFIDKEIQDSKQQFKAATDFILKNEDKTERVEN
                     LHFETEKYKDHPVDYQPLTEKGRNYLKEERKLPEWLIDYAEKEGLIAELKPKYERQNF
                     LVRDDRLDHAVAFLWKDPQTKETVGASYQGTFIDYERFGERGTYKHIDKNSTANHGFN
                     LKIGDPKQLKFFESSIDLLSYAALNRDQLNDTWLVSMEGLKHHVISHYFGEAVSELRK
                     KQAFPQSIEICVDNDRAGHIFYEKEQLMGAVDPFTNQKVRCERGIANDWQVPKEYKII
                     YEEVAKEEKVTPEAIMAIHKTENNLQLTNQLVSAHKVNAFFGQQLSVNDSIEAINLKD
                     ICREVAKELKVCERVDGTYDFDRFYQEKGDINAQILFSYKAEQYYKGYKNHEHEFIPE
                     VKKDWNDQLKHEIQQQEIRKQKRAMLFQQGRQQERE"
     gene            35068..37272
                     /gene="topB"
                     /locus_tag="ACG842_000544"
     CDS             35068..37272
                     /gene="topB"
                     /locus_tag="ACG842_000544"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HFQ0247107.1"
                     /translation="MKTVILAEKPSQAKAYADSFSKATRKDGYFEIQDRLFTGETVIT
                     YGFGHLVELDSPDMYDEKWKQWALEHLPIFPNQYHYHVPKDKKKQFSVVKRQLQSADT
                     IVIATDSDREGELIAWSIIQQAGANQGKTFKRLWINSLEKEAIYQGFQQLRDAEETYP
                     KFEEVQARQIADWLIGMNGSPLYSLLLQQKGIPGSFSLGRVQTPTLYMIYQLQEKIRN
                     FQKEPYFEGKAQVIAQNGAFDAKLDPNETQATQEAFEAYLKEKGVQLGKQPGTILQVE
                     TEKKSAASPRLFSLSSLQSKMNQLMKASAKDTLEAMQGLYEGKYLSYPRTDTPYITEG
                     EYAYLLDHLDEYKHFLKAEAIPTPIHTPNSRYVNNKKVQEHYAIIPTKTVMTAAAFEQ
                     LSPLQQAIYEQVLKTTVAMFAEKYTYEETTILTQVQQLQLKAIEKVPLDLGWKKLFGK
                     ESDGKEKEEEPLLPKVTKGEMVTVDLQVLEKETKPPQPYTEGTLITAMKTAGKTVDSE
                     EAQSILKEVEGIGTEATRANIIETLKQKEYIKVEKNKLVVTNKGILLCQAVEKEPLLT
                     SAEMTAKWESYLLKIGERKGTQTTFLANIQKFVSHLLEVVPGQIQSTDFGSTLQEVKA
                     ASEKQEAARHLGICPKCQEQEVLLYQKVAACTSEACDFKLWTTIAKKKLTATQLKEII
                     QNSRTSQPVKGLKGQKGSFEATIVLKEDFTTGFEFSEKKKTNFKKRTRRTTK"
     gene            37288..37437
                     /locus_tag="ACG842_000545"
     CDS             37288..37437
                     /locus_tag="ACG842_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0247108.1"
                     /translation="MRNTTHKQQEIENQKAKIRSEQEKMRASKRRLQQLKNQQRIQKE
                     IELDR"
     gene            37511..38104
                     /locus_tag="ACG842_000546"
     CDS             37511..38104
                     /locus_tag="ACG842_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HFQ0247109.1"
                     /translation="MKDKIQCIFDKHNGNLSLKDARSEGLSPMTLDRLAEKDKIEKVA
                     PGFYTLKDAFVDELYLAQSIYSRGIFSHETALDLFQVGTYIPKKINMMFPKGYNVSKE
                     KLDQYAIQPHYTNKENFDLGVTETESFYGNKILVYDLERTLCDMWNPRYKASVEVKQE
                     ALKEYMTSTSRNTNKLRRYMDILKSPKEMTLYMLPLY"
     gene            38120..39019
                     /locus_tag="ACG842_000547"
     CDS             38120..39019
                     /locus_tag="ACG842_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HFQ0247110.1"
                     /translation="MTPTQFKEKTRNFAAEKHLNPQIVQRYYMMEKLLECIVESPYHD
                     DFVLKGGFLIGSKYGLENRSTVDIDTTYRNSKLTKEKLETVFSELTANPTKDGIRFEL
                     KGLTETREADYYPGFQAKFIAYLENARVPFKLDITSGDSIIPAALMYEHKLMFEDKTI
                     HIPAYPTEQILAEKLHATFSFGKDNSRMKDFYDLYMIPKLERIDEKNLYKSVQGTFKE
                     RNNQSVFKHLYDQEMPQLVKDEGLREKWKRYQQENYFAKDISFESTLTSVDSLMQSIV
                     EQEKAERKQNFKRSQQMNQEMER"
     gene            39022..39507
                     /gene="ssb"
                     /locus_tag="ACG842_000548"
     CDS             39022..39507
                     /gene="ssb"
                     /locus_tag="ACG842_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFQ0247111.1"
                     /translation="MINNVTLVGRLTKDPDLRYTASGTAVATFSLAVNRNFTNQNGNR
                     EADFINCVIWRKPAETMATLAKKGSLIGVVGRIQTRTYDNQQGQRVYVTEVVADNFQL
                     LESKAATESRMHADQSSSSPSPTTFEQRDTATPNNNGLNASQNPFGGQSIDISDDDLP
                     F"
     gene            39873..40133
                     /locus_tag="ACG842_000549"
     CDS             39873..40133
                     /locus_tag="ACG842_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0247112.1"
                     /translation="MNKYVGLLDKIRVIKTQPLLVRFTLQTIHESINCVVADIEIIDK
                     LLIMDDGKYNIAVTGHFNKRNQLVIASMYVRNPDHFTRSMGI"
     gene            40574..40780
                     /locus_tag="ACG842_000550"
     CDS             40574..40780
                     /locus_tag="ACG842_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrA"
                     /protein_id="HFQ0247113.1"
                     /translation="MRKKFDYWGVPFSELFPNYHAPHTVECDCGERAKCIKSYRLYQC
                     PTCGKKYTLSYGDYILIDEKGKKR"
     gene            40780..41031
                     /locus_tag="ACG842_000551"
     CDS             40780..41031
                     /locus_tag="ACG842_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0247114.1"
                     /translation="MKTQHKLLTVKEVVQILVDKEVISEKVAEALLIPRYWTIETPLV
                     EDAPTMLFEDLVKIAGYELHYQENMETYEIEYWLTKLEN"
     gene            41046..41483
                     /locus_tag="ACG842_000552"
     CDS             41046..41483
                     /locus_tag="ACG842_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0247115.1"
                     /translation="MAQNYYDEFVKLPLDKMAQKMEDMTFLYHETRVPKKHYKEKLSV
                     AVEEMIESGVEMNLIATYYRTLEELKKQNGKWFFQALLCLEVGVKPSTIKPSEYQALE
                     LTYAKFAQIKKAKAMASDWLDTFESIEKNGAFYTIESEEKDNE"
     gene            41476..42183
                     /locus_tag="ACG842_000553"
     CDS             41476..42183
                     /locus_tag="ACG842_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0247116.1"
                     /translation="MNKVVYQLTTEDVSQFLTSKEHLNEVIRRIQKDFSCMEEMKYFI
                     QRVEEQLYMESETSQTKVTVSHNHLDVYKRRPETVKKGTELLRLETEKYIMNVYLERS
                     ELVENFVSYTICRKHYYSESEYYSIFESMELMEEEMEELNSKNATVDDYYLVFTRFLT
                     CGTESIVLMGMDYELAVPEAFLREYVEEVDNPGDDWYTTLTDFLDTYTYDDTEEIQNE
                     LDRKRMPYQRFERKKGK"
     gene            42348..42548
                     /locus_tag="ACG842_000554"
     CDS             42348..42548
                     /locus_tag="ACG842_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0247117.1"
                     /translation="MNKIIPLLFVLFVGEILMRHAAAVHLNFVVFLGILVFCLIFWRF
                     VLALVSLLFKMIAAIFFIGLFR"
     gene            42564..42743
                     /locus_tag="ACG842_000555"
     CDS             42564..42743
                     /locus_tag="ACG842_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0247118.1"
                     /translation="MPIAIGNKRLPVTLDEKRQKELQQLKQKYGKSESKIMCIALDLL
                     IAQEKAGFDIPALKK"
     gene            43084..43347
                     /locus_tag="ACG842_000556"
     CDS             43084..43347
                     /locus_tag="ACG842_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PbsX family transcriptional regulator"
                     /protein_id="HFQ0247119.1"
                     /translation="MEMLPIKKWGNSNGLRLPKYIMEYLGIHTEDKVKIIQEEVNGQK
                     RLIIEAANIENELTIEQLFENYTDERNHVTIQNLGEAVGNEKW"
     gene            43341..43691
                     /locus_tag="ACG842_000557"
     CDS             43341..43691
                     /locus_tag="ACG842_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016610020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFQ0247120.1"
                     /translation="MVRKPRQGDILLLNTAPRSGHEQTGKRPYIVLSHDIIADYSNVV
                     TVAPISSTTRNYPLYVNINSEYEMKTTGKVLLDQLTTIDYEARECIFLEKAHDSLVEE
                     LLMKVRTVFQKVSK"
     gene            complement(43715..44329)
                     /locus_tag="ACG842_000558"
     CDS             complement(43715..44329)
                     /locus_tag="ACG842_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFQ0247121.1"
                     /translation="MRVAYIRVSSIDQNEQRQIEEMKKFGAERIFIEKQSGATITHRL
                     VFQEALDFVRDQDIFIVEAIDRLGRNYDEIIASVNYLKKKNVQLIITSLPIMAEAIGN
                     PLLDKFIKDLIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSANAKDPQ
                     RRLVYKNIVEDLKKGVAIAKIAKDYNVTRQTVYRIKKDSMVNHE"
     gene            44643..45065
                     /locus_tag="ACG842_000559"
     CDS             44643..45065
                     /locus_tag="ACG842_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0247122.1"
                     /translation="MKNKFILSLGILTIGISVTGTTIPTFADSTNEPTTVVDNNQELT
                     PLDRNGIAIGNPTNDLTVTENYLIVSPLLARAASKTIRVTGTMIFDKVGWPSRPPQSR
                     NWKEKRNGYWYYGKLYLQSYKSAGNNWIATYSGTLTRK"
     gene            45075..45278
                     /locus_tag="ACG842_000560"
     CDS             45075..45278
                     /locus_tag="ACG842_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0247123.1"
                     /translation="MPEFVNTPNFYQDSDILSKINLPKEESALQVQRTLQETQKIQKS
                     ISNLEDSEEKRKKRDQKTYQKEK"
     gene            45489..46073
                     /locus_tag="ACG842_000561"
     CDS             45489..46073
                     /locus_tag="ACG842_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFQ0247124.1"
                     /translation="MKIGYARVSTGLQNLDLQEDSLNKFGCEKIFTDHMSGAKSNRPG
                     LEMAIDFVRSGDTLVVWRLDRLGRNMEDLISIVNRLNERGVSFHSIQENITMDKSSST
                     GQLMFHLFAAFAEFERNLILERSAAGRAAARARGRFGGRPEKLSEQDLELLKTLVDSG
                     TPIKTIAERWNVSRTTIYRYLAKMEEKNGKTNSH"
BASE COUNT        16937 a         7300 c         9359 g        12610 t
ORIGIN      
        1 tcgtatgaat gaagcacctt cgatgtcatt ggcaacagag atttcagata agatagacaa
       61 ttgattgtcg tctgttaaaa aacaaacatc tagtttttca gagctaacgt caataccgac
      121 aaataatttc atggaagaag gacctccttt cgatataaat tttggaaaat gcttcgatac
      181 tgtgggattt cccagaaact cactgtatag ccacaacctc gcctaataga atacggtgat
      241 aaaagagcta gaagctgcct tccaaactac tatttgaaag tctagtccat tatcgaaaca
      301 gctaatgagt tggtagtgat aacagtggtt cgggtaacag actaaacagg gtagacaaac
      361 gctacaggag gaaatagcaa attcccgttg gatcctttcc atacatccat tgtactctga
      421 gaaatcacac aatatcgaaa caaattccgt taggaatgtg ttattcaata aattttataa
      481 tctagaaaca ttttgaataa aaactattag acaaatgata ttttacgagg aggaatagta
      541 gatggcgcga ccagttttag aggcgatgga tatggatact ttgacaaaag gaaggtatgt
      601 acagctgtta gctcgtggtt attcacctaa agaagtattc aaagccttat ctaaaggaac
      661 agatatggaa aaagaaagat tgcgaaaaga attcgattat tggagaaccc ataatggaat
      721 caaggatttg aaaccagcac ggccagtaat taaacgaaag aaaaagataa cccaagactg
      781 aagtgtagag cgtatgtgat cataggcttt tttatttttg caaaaaacaa ggaaggtagg
      841 aaaactcatg aattatgaag aaatcgaaaa cagaaaaaaa gtgtcgaaag aaatggaaga
      901 aaagttacta aaaacgatga agcaaaaaca ccttaaaaga ttatctgtcg cgcaatatat
      961 caatgatatg cagttaacag gaaaagagaa agcttgtttg ttaggttcaa tgaaaaactt
     1021 tgaacagttg agacgaactt atagaattca tttttaaaac tttttctatg gaaatgagaa
     1081 aaagtttttt tgttttgtct gaaaggaggc agatggatga aaaaatggtt atttggtttt
     1141 ttaggagtca ctttaatcgt cttttgcagt gtgtttggct atgttagcta ccaaaaacat
     1201 gaaggagagg tgtttaagca aaatatagaa aaaaagatgc ccgtagatca aatcaatgca
     1261 catgcaaaga gttataagga ggatgcaacg aacgtcaata atgatatgtc tttggggcaa
     1321 atgttgtcca tacaaaaaga agcaatcgaa atgggcgtca ataaacaagt atttgcacaa
     1381 attcagattc ctgcacttgg tttagcattg ccaattttta aaggggcaaa tcaatacact
     1441 ttgagtcttg gagcggctac ttatttttat gaagatgccg aaatgggcaa aggaaactat
     1501 gtcctagcag gtcataacat ggaaatgcca ggtgttttat tttctgatat tcaaaagttg
     1561 tcattagggg aggtgatgga cttagttagt aacgatggcg tctatcgtta caaggtaacg
     1621 cgtaaattta tcgtacctgg gtactttaaa ttaatcgatg gcgttcctga agagaactct
     1681 ttcttgtctt tgccaaagaa aggagagaaa cccttattaa cgttgtttac ttgtgtttat
     1741 acgtcacaag gaaaagaaag atacgtggta cagggagagc tacaataaca aggttttaat
     1801 caacgcgagt cgttgaaata tgaaatgagg agagtaaaaa atggcaataa atcggtatgt
     1861 aaaaaaagct ggaatgcttg ttggaatgtt aggaatcatt gcaggctgct ttcaggggta
     1921 tcaagtacat gcagaagatg tcacgcaaaa aacaccacca gagaaggtca atatcacggt
     1981 tcataaatta atgtacgatc aaggaacgca attgaatgtt gatattgacg ggatcaaaaa
     2041 cgatggttat acgcacgatc aatatcctac aggtgtaacg aaatacaaca aggcagatta
     2101 tggtgatgtt gagtttacgt taggaaatat tacggatcaa gtattaccga gagaagattc
     2161 tgatctaacc aacgcgaaag tggacgagat tgtacaagat gtagaagaca agggatctaa
     2221 cagtgagtac gtcaagaatg caaccaacaa aattacttca gctgtagatg aaaatggtga
     2281 gatcactttt actgatcaac cagcctatgt caattccaaa gggaatgtat atgtggttta
     2341 tgaaagtaaa tccgctgctg gattagtcac acaaaaagca aaaccaatgg ttgtgattgc
     2401 gccaatgaca gataaaacag gatcaggatt tttaaaaaat attcatttgt atcctaagaa
     2461 cattgtttct aagttgagtt ttgaattaac gaaatttggt gacgatggcg cagaccaaag
     2521 caaacaaacc cttttaaaag gggcaaaatt tgagttatac aagggcgaac ctggaaaagg
     2581 aacaaaattg ggtgatcttg tatcggatga tcaaggaaag ctgactgcga ctgatttgac
     2641 gttaggaaaa tactattttg tggaagttcc ttcggaagtt gtagtgggat cagatcaaga
     2701 accaacagca gatcaatatt tattaggcgc agacgctcgc aacgatgcgc acaataaatt
     2761 aacctttgaa attacgaatg atggcgtgac aagtgactta aaagcttcat acgtaaacta
     2821 taaagcacca gtgatcgata aaacagtaac aaatggaaca ggacaagaac attctttcca
     2881 aattggtgac gcagtaaatt atcaaggaac gattcatatt ccaacagaca ttgcaggtgg
     2941 cgcagacggt atcacagtaa acggtgtgaa aagcgaaact tctccttaca gtgtatttaa
     3001 atggggagat acagcaggta aaggactttc ttatgttgct gaaaaagcag atattaaagt
     3061 aacaaataat gatggttcag tcgtattaaa agaaaatact gactataaaa tcgaaaattc
     3121 tgatagtggt tttgtgatcg attttatcgt aaataatgga caagtaagtg atacagttgc
     3181 gaatttacat ggtcaagatc taaaaatgac ttataacatg tttgtgaatg atagtgctga
     3241 aatcgcaaat ccattaacaa atagcgtgga tttcgtttat aacaataatc catttaatca
     3301 ggaagaacac cacgaaaaaa cgaaagctga cgttgtgact tatggcgcaa aattcttaaa
     3361 agtcgatagc ggcctatttg gaacaggtat caaagcaaca ccgcttgaag gcgcagaatt
     3421 tgcagctaag aatgcagaag gtaagtacta cggtggactt actgatacag ataaagatgg
     3481 tgtcaaagaa gcagtttggg tagacgatgt agcaaatgca gcaatcctaa aatcagataa
     3541 agagggtcat tttgaaatca ctggtttaac tgaaggagat tactcactgg aagaaacaaa
     3601 agctcctgaa aactatcaaa aattaacaaa agagatctct ttcaaagtag acaaagattc
     3661 atacaaagaa gaaaaccgaa tcacgatcaa aaataatcaa aaagcttccg taccaatgac
     3721 tggtagcaac ggtttccaat cttacgtatt gatctcttgc ttgttgctag gagcaggggg
     3781 actttctgca gttgtttact ttaagaagaa agcctagaag gatcagctaa tgattattcg
     3841 taagccccca aaaagaagag tcatgcacaa actgatgata tttttttggt tgctgtcggc
     3901 agtagcagtc tatttaattc cagttgtgga aacaaatgta gcgaaatttc aacaacagca
     3961 aagaatttta gccattgaaa atcttgaaaa aacagattct tctttggatg ttgcgaaaat
     4021 agagttaggc gatcctgttg gtgttcttac aattccaagt atttctttaa aactaccaat
     4081 ttatgacgga acttccgaca aaattttaga gaatggtgta gggattacgg aaggaactgg
     4141 cgatattaca ggaggaaacg gtaaaaatcc tttaatcgcg gggcacagtg gactttataa
     4201 agataatctt ttcgatgatt tgccttcagt aaaaaaaggg caaaaattct ttattaaggt
     4261 aaatgaagaa caacatgcct atcaaattta tcgaattgaa gaagttaaag cagaagaatt
     4321 acaaaaaaat tatttagatt atcttatgcc tgaaaaagac caagatagga tcacgttaat
     4381 gacatgcaca ccaaaaggtg tcaactcaca ccgcttttta gtgtatggtg aacgagtttc
     4441 cttcagtaaa gaagatttaa aactacagaa aaaagcaaaa aataggaaat caatttcatt
     4501 ggattggcga ttagcactgc tattcgcttt tttttgcgtt cctattattt ttatttataa
     4561 aaaaataagg aggagatcgg aatgaataag agaaaatggc tgtttgtttg tgtagcagct
     4621 ctcttttcta ctgtagctta ttcgcaactg tctttcgctg acagcagtga tagtagtacc
     4681 actcaaacaa tagataattc aattactgca ccaagcgaaa cggacagttc ggagaatact
     4741 acagaagaaa cggcagattc aaatataagc tttactgaat caacgaatcc aacgtcagaa
     4801 agttcttcag agacttcaga aaagccgcaa gacgtagaag atacgcaagt gatcaagcaa
     4861 gccattcata ttcaaaaaat tatttcttcg aatcaaatca acaatcaagg cagtcaagta
     4921 gcccagcaag aaggtatcaa tggcgcgaag tttattgtct acgatatcac gaatatctta
     4981 gaagaaatgt cacaagatga taatgagaaa aatcaatctg tagaaataat tgagtcaaaa
     5041 ttgaaagatc gagcaaaaaa actttcatac gaccagttga aaaaagtggc tgaaggagaa
     5101 accaaaacga ttgatgatca ggaaggcgtg attgaatttt ccattgaagt acctgcaaac
     5161 aaaaaacaag catactatat cgttaatgaa tcaagccctg aaaacatttc aaacagtgaa
     5221 gatattgttt tattgacacc tgtttcaaat gaaaaaggtg aatttttgac ggatgtttgg
     5281 ttatacccta aatcagagaa atcagagccg aaagaagaaa ttaaaaaagt tgtgtcaact
     5341 ggtgtaaaga agaacttttt tgaaaattgt tgggaatttt ttgctcaatt attttttgat
     5401 aattaggatg aaatgatgaa aataataaga atattagttt ttaatctatc ctttttagcc
     5461 gcaacaatat tgtgttgcgg tttaattttt tctcaattat caatttatcc aagatatcaa
     5521 cgattgttca ttcattttct aaacattcaa tgtgcttcca tgccattaga ggtgattaaa
     5581 aagctcttga tcaatccaat ttttatggtt agtttggttc ttcttacagg attggtaacg
     5641 tgggtattag gaaaaaaaga atgaaaagct tacgatcgtt taaaaaaata ttagatggag
     5701 ggcattatgg aaaataaaat tcattgcact acgggaagag taattacttt agtgttgttt
     5761 ttgttccttc ttctttttgc gaggggaact gcagtccagg ccttagaaaa agttgatccg
     5821 agtcaaatca aagaaaataa taatctggtg ctttatcatg atagtgattc attgagaaat
     5881 atcgaagtaa ctgggatcaa tggatttaag atactagatg agtattcaac taaaaaacaa
     5941 aaagtacttg cttggccaaa agtcaaagat ttgaaaagta cgtcaaaaat cgttgcccat
     6001 tatcaaaaag taggtactta tctaggaaaa gaaattgatt gtgacgtgac tttttctaat
     6061 tttgtagcgc acgctggggt taaccaagac ggattagcta aaacaattaa tggcaaaaag
     6121 tattataatt atttgctctt tgataaaaat atttatcgag gatttttcat gcagtcaatc
     6181 aatgtcatga atgcaaaact agaatttaag tatgcaggaa caaaaacaaa agtagctttg
     6241 agtgaaccac agccagaaat tcaatcaaaa atgagatcga tgagagcagc tggtattgat
     6301 ttaaacgata catttctatc catttacagt ttaaatggac agtatcagca acgtgtgtct
     6361 tggtataatg acaataatga atctgtcggt gaacgtaata ttgatatgag agaatttgtt
     6421 gggtatgaaa aaatgggtag cttaccttat tttgtcacaa cagatacagc atgtgcagaa
     6481 tacaaagctc ctgcgttatc aacaaacaat ttaacttcaa aagtagtggg aggacgtgca
     6541 gaaaaggctt atagctcgaa tgatcatttc accgatgttg taggagctga tacttatcac
     6601 agaagtggtg taacgtatac gcttcaaggc gcttccccaa cattcatgat tggcgcaaat
     6661 acgaatagta tgatgtttag ctttgatact gcattgctat ggacaccaca accatcgaag
     6721 cctacaaaag aagtgtttaa caaagctaat actgaagagg cagcacacaa tattgacaaa
     6781 aaagtgattc cacaaggatc agatgtttac tatcatattc accaaaagtt tgatgcatta
     6841 acagtcaaca caatgaacaa atacaaatca tttaaaatca ctgatacctt tgacagcaaa
     6901 aattttgata tggtatcgga tgggaaaaac tatgatggcg cattgtggta tgttgaagcg
     6961 aatggtactt ggaaaaaagc tgatacagga ggaaagttaa ctgtatcagg taatacagtc
     7021 acttatactg cttctgacag ctttttagct aacgctgcaa attacggtag ggattatatt
     7081 ttgcagctcc atatgagagc aaaaaatgat gcaaaaggaa ttgcaccaaa tgtggctact
     7141 gtaacgttta acaatgatcc gaacaaagga acacaaaaaa caaatacagt agaaaactat
     7201 ttctctctta aaccaaataa aaaagtggaa caagatggga aagatgttga tggtcgaaat
     7261 gatggtaaaa aaggaacgcc tacagctcct ttaaatgcag gatctgaagt acaatatcta
     7321 gtcacacaaa aatggcatac aaaaggtgta gacattgcct cagatcatta caagcaattt
     7381 agtattcaag atccagtgga agatcgtttg acgtataaag aaggttctgc gcgagtgata
     7441 gatatgagta caggaaaaga tattacttca gaaggtacac taacctatga tagcaattca
     7501 agaacgctga agtgggaagc ttccgctgat ttcttaagta aaaatctttt agatggacga
     7561 gaaattcagc tgatatttac agctaaaact cctttgaatg aagaattgga tatcgacaac
     7621 caagcaaaag tttcagtaga aagtattgag aaagcaacta atgtagtaac gattggggta
     7681 aaacctaact tacctcagat cattgtgcca aaaactggat cgaatcattt agtgctaact
     7741 gttattggaa gtatattatt tcttacttta gcatcattgg gctatgtagt tttaaaactc
     7801 aaataaatat aaaaacaagg ctgagaatca gccttgtttt cttttatttg aagattggat
     7861 tcgcactaat aaatacatta tctacgccaa ttctaccagc agagtcaact acaaaattat
     7921 gacctaatcc gagaatactt tggttattat aaagtaaatt tgcattatgg gcagggctat
     7981 ttttccatcc taccatggca tcgatagcac catcgtctga taaaaggtca tcaatataca
     8041 gcccagtttg acttatgttt tctcccttag caccgtagca gcctaagctt tccaataaat
     8101 cttggaaagt accaccacca gggcgatagt gactgaagct gacttgtaat tctttagctc
     8161 gaatatctgc agcctgttga acaatatcat tgaattgcat tggggcaaca ccattttgtt
     8221 ggcgataatc atttaaccaa gcaagttctt tttgacgcat aaagttacgt aattcgtcta
     8281 atgaaatctt tttatatttg aatgtgattg ttttattttg atcgacgtta ttaatggatc
     8341 gttggccgtt tttatccacg gtaacggtag gatcgtcatt tctgcacaag aaataatttt
     8401 gatcagtcat gtttttccaa ggagtagcaa agctatcccc ttcgtatact gtttttgatt
     8461 cactagagag aacagcgttg gtatcagcat ttacatgatt aacagtcaca ttaaatttac
     8521 gagtgtagtt gaaaatataa ttttgacttt cagtcacatt gtttaaggtt actgaatttg
     8581 ttggttctac ccgatcgtct aaaacatatc cttgaataga aggagcagtt aaagtgacac
     8641 tgtcaccgtg agtgacattg actatctgtt ctttcaaaat attaactcgt ccttcgtttt
     8701 tgtagttgtt attgcacaac aaattgattt gatgcacttg attataattg aatgtgattg
     8761 ttgtattacc tgtgattgtt ttgctttgag tatcattacc aatcactttg tagctaaaat
     8821 tatctttagc gccttcgcta tatttaaatg cttttgcctt agcagtgtaa gtagaaccag
     8881 aagttacttt tgcttgatct gtagcaatca cttgacctgt ttgattgtca atatggttaa
     8941 tagtaaccgt atattctttt ggtgtttcag gtttgtgttg caacccttta aatgcagcat
     9001 caagactatt tttagcattg tttacttgtg tttgagaaac cttagaatcg tctagtactt
     9061 ttttagcatt ggataaagca gtctgaaaat tattccaaga ttcttctgta aaatctcctt
     9121 tagcagttga ttttactcgg ttgtataaag cttgtaattc agctttattc acagtaggaa
     9181 ctggtttttt ctttaatcct ttgtaagcag aatctaattt atttttggca ctatccactt
     9241 gcttttgagt cgcttttgag tcatttaaca ctttcttagc attgtttaaa gcggtttgga
     9301 atgcgttcca ggtattgtcg gtatagtcac cttttttcgt tcccttaacg ctcgtataaa
     9361 gagcttctag agccgattta ttcactttgg gtttagaggt gtcagcgcca taccaaccga
     9421 taccttcgta tttccagcca tagtctttgg ttaaccattg tacttctttc caacttgcag
     9481 tgtagtgatg tgagccagat tttacaccag gaaggaaaag gcggtagatc ggtttattat
     9541 ttttttcggc agaacgccag gcaggccctt cataagtcca gccgtaattt ttagtcaacc
     9601 atttcacttc atcccaacta gctgtgtaat ggtgatcacg aagaccttta ttgtacaaac
     9661 gatagacaag atggccaccg ctagtcggtg cttcccaggc ggggccttca taatttcccc
     9721 attttatctt tactagatgg tcacgttctt ttttgctaga ggtgtaaaaa tgttcattat
     9781 tatttggatt gtaaaggcga tacatgacgt tagttgaagc agcgtcaaca gatccagcaa
     9841 gactaagtga ggcagcaaat gttccaatag ctaaaacagt ttttggatta aataatttca
     9901 ttttttcaac tcctaaattt aattaaaaat gtttacatga ttattatata tcggctaacc
     9961 gatatagtca ataaaaaata agaaaagcct tatataattt tggcttttct tattttattt
    10021 gttagttgaa gtattaatat caacgatttc ttcagccaat ttatttaatt tcatagcatt
    10081 tatgaatctc atgttgtcta tactagagcg tttgttggca aggtcactta ctgtagttaa
    10141 gggtacgcca gattctatgg atatagaacg aactgattta ccactatcaa acagcaacca
    10201 ttcgattttc tttttatcag cgtacatttc aatgctcctt cttgtttttt ctttattata
    10261 gctctttttt gtaatgaatc acaatatcaa gagaaaaatt ttctatagaa aggtggattt
    10321 ttatgagtat tcgttctccg tgtatccaaa tgaggtttgt tgctactttc gttatttgat
    10381 atactaaaaa taaacaacga aagaaggaat aaaatgaaga aaaagcaact atggtttgtc
    10441 tctatgtttg caatattttt tattatttta agtggatgtt caagtaataa gaaatcggaa
    10501 gctaaagaag ataatagttt attgcaacca ggaactgttt ggaaagaaga agtaggtggt
    10561 cttattcata atttaaaaat tatagatgat actacctggg aatattctga aagtgtatgg
    10621 catccagatc ctgtacaaat aacagttaag aggcaaaaag actatgaagg tttagaaaga
    10681 tacaaaatag tagattctgc aggcattagg gaatttataa acaaacctga ttcattattt
    10741 attgttgttc cctatgagaa ggatggcgta aaaaaaataa tttttcttgg atctagcaag
    10801 gatgagaagc gaacgaaaga gaaattaatt catgatggat cagagttaaa taaatacaaa
    10861 ttacaaaaga ctagtgagtg attgctagtc tttttattta tcctgaagga ggaatcacag
    10921 tggcaaaagt taaaacacca ttcgaaaaag ccgcgtctaa aatcttagaa cgccgtggtg
    10981 tttctgaaat acagtggaaa aaagaagttc ttgaaaaagc acaatttgac ctattacgag
    11041 gaaaagataa ggacttagaa gaatatatct atcaacgtga acgtgaaaaa cttgtattcg
    11101 atgaaattat taaacattaa aaggagcgaa taatcttatg tttgaatcaa ttacaatgaa
    11161 ttttttaact caaccgaaag tagtagctgc agttgatatt tttagagcat tacaaactgc
    11221 cgcaactagt ttgaaaaata atctgatgat ttttgcggct gtagctgccg ttgcctccat
    11281 tgctcttgga ggtattttct tcttctttgg gcgaagagga gctgaaaacg ggaagacaca
    11341 cgtaggttat gtattgttgg gggtaggagt tattttagct gccgcttcta ttgtagtttc
    11401 aatgttctca atgtttggac aaaccccaca aggactataa aaaaaacaag taggaggagt
    11461 aaaaagtacg tggctacttt ttactccttt ttttatgaaa ggagaatatt catgtttttt
    11521 acgatttcag gaaatgcaaa attggataaa gagttaataa gaaaagcaac gaagaaagtg
    11581 ggaaagcgta cttccttttt accgaaaaca attaaatatt ctgtcattga aacggaagaa
    11641 cagctacatg tgttaggaca agtgttagaa caagagaatc aatcttctgt taaattgact
    11701 ctcacgttac atgataaaga aggaaaaata gaaaaggctg gcacagaata cgtttttggc
    11761 ccactggatt atcaaatttc agaaggtttt tatcctacat tgatagattt aatccgcctt
    11821 gatgtgttta aagatacgga tgaaactatg agtgtggaag agtcttatcg aaacgaaaaa
    11881 ctgctacaag ttttagaaga agagttagag agcgaccaaa gaaaagtgaa tcaagtgagt
    11941 gaagtcgaag taaatgaaga agtaccatgg gaacgaccaa tctttccaaa gaaggagtca
    12001 aaagaaaaag agacagtcta tgttgacgaa caagctgata ttccgactca gaaatcgata
    12061 tttgaggaag agaacgatgt gatgggtcaa ttggaggtag atccctttga agaaacaatg
    12121 gaggatgaag agacggacga agtagaagtt gatccagttg aagctaatga agagtcaaca
    12181 tggactgaag gggattttca ggaatatatt gaaccactta tcagtgaaga agtagaaaaa
    12241 aacgaaacag agatacaaaa ggcaactgta tttgattata ttttggatca atatccgcca
    12301 gagcatgaat ggatgaaaga aaaaaacaaa ttatttttaa atgagcttta ttcgaaatat
    12361 caattacccc aaacctatca agcattttta gagagtaaag aagaattaat ctcacagggt
    12421 aaaggcacac ttttagaaca gttagagtct gtccaatcaa caaattggca tgagcttgca
    12481 caagaagatt tattagccac ctttgaagaa cgtcaacaag aaacagatca agaaattaat
    12541 gagtatgcga cggaacagtc gtctaattgg gagaaagtaa aagaggaact aaataaagaa
    12601 gaacagcgag tgattgaaga agaagtacaa aaagtcaaag agcggtacga aaagaaacgc
    12661 gacaatgctt attccttgtg tgtagaaaag atcaacaatt ttactaagac gaatcaagaa
    12721 gcgttatcga aagaaaaaga aaacttgtta aaagaacgta ttaaagaacg aaaagaagag
    12781 ttctaccgcg gtttacgtac agataagcta aagattagca aagaattaaa tgaacaactg
    12841 aataagctat atcaaacaac gactgaagtt cttatggaaa aacacgaaca gatccagcaa
    12901 gaattacaag tacaaattcc aaaatggaaa aaagagtatg aaattcaaca acaagaattg
    12961 aaagaacaag aagaaaaaca acggcaaaaa gaaaaagagc gtgaacaatt agaaataaga
    13021 aaacgggaac aggatcttgc ggaacaaaaa atggcgatag aaaaacaacg gttagagcaa
    13081 gaaaaggaaa aagaagcagc tgctcgtaag gaacgtcaag aacaaatgga tacattgcgt
    13141 caagcacagt tgaattatct agcacaacct caaccagtag ctgctgttca aacgccagtg
    13201 aacaaccaag caaatttaac tccttcaccc cagcgttcct ttaacggatg gatggtggga
    13261 tgtattgcaa gtttaagctt gcttctaggt ggaggaacag tatttgcttt taatcatatc
    13321 aatcaatctt cacaggctga ttctgtagcc acaacggcgt caatcaagga agaagcccag
    13381 gaacaagcaa gaaaagagta tgaacaacaa ttagaagaat tattgaatcg taccaatcag
    13441 tcaacaacag agtcaagcat ggaaaatagc aaagagagtg atcctagtaa ggctaaagaa
    13501 ggatcaagcg attcaactgt acactcaaac acttccgaaa gttcagaatc aaatgaggtg
    13561 aaataatgcg tattagagaa acaacgagac gaaaagacaa acgattcaat ttagaaaaga
    13621 ataggtatct ttctcatttc tatcgctata ctccttcagg agaagagcta ccagaaaaac
    13681 atgacaaacg tatttctcaa aagatttttt atagtggctt agcttatctt cctttaaact
    13741 atgttgttac aagaatacca gaagcatttc atcgatttga gattattcca ggaagtttac
    13801 aaggagtgaa tatttttcag ttacaagaaa tgccgcagtt attccatttt gattcaactg
    13861 gtttttggaa tcctttgacc ccaacaacaa gtttaggcac acgtttagtt gtttctacga
    13921 tttgtgttgt tccagctgta cttatgggat tgaaaatgga catgtggttt cgtccgctag
    13981 cagatggaca aaaaggggat aatcgattaa tgaccttaaa agaaatccaa cagatttatc
    14041 cgcaaatacc tgaaacaaaa actacttttc caggttatgg aggtattcca atcacacatt
    14101 acaaaaaata ttggtacatt caaacagata ctgttaatac gtgtattgtt ggtacctctc
    14161 gatcagggaa aggtcagatt gaagtgttag ccaccattga caatttatct cgtgcagaaa
    14221 aacaaagttc aatggtcgtt aatgatccaa aaacagaatt atatgttggt agtaaagatg
    14281 aactagaagc tagagggtat gatgtatatg cctttaatat caccgatcca ctgcaatcaa
    14341 tgagtgataa tccattggca ctcatcgtga aatactggaa acgaggagac atagatactg
    14401 caacacagtt aaccaatact ttcacaaata cgatttatta tgatgccaac gcttcagata
    14461 acaaatactt taacgaaaac gcgcagaaag ctgtcaatgc gcttattttt gcgttgttag
    14521 aacaagctga tcaaacagga gattactcca aattgacacc gaataatctc gttgagcttc
    14581 tttctgagat tggcggtttt aattattatc cagatccaga caatgaattc aaacagttga
    14641 atgccttaga tgaatttatg aatcagttgc cacctggaaa cattgcaaaa aaacaatatg
    14701 gggcaacgaa aattggatct gataaagcga agggaaatat tttatcaacg gcaatcacgg
    14761 gattgaatcc atttactcta acgaaaattg caaagatgac ttctcaatca acgattgatt
    14821 ataaatcggt tgggtttcca aaatacttag atttgaaatt acatgaaagt cttttaaatc
    14881 aacgagtcgt agtagaattt tatcataagg gaaagaaatt tcatgaagaa cgtgtcaaag
    14941 taggctataa aggatttagt gaaatcaatt ttgattgtca tttagttgca ggtgatcaag
    15001 tttgtttaaa atacgatcat caaggaaaag aatatgtggc ttggtatgaa ttttctcaaa
    15061 gagtattgaa agacgaagag ggtaatatcg tttatcggaa aaaacgagga gaaacccatt
    15121 tgccagaatt agaaaaagaa gccgttctaa cattagatga ggaaaaaagt aacttattag
    15181 tagagtctat tcggatgcac tataccgata aaccaatggc tatttttatg cttacacctg
    15241 attatgatcc atctaaccat atcattgtta gtatttttct tagtcagtta tataaagaat
    15301 tatctacgca atgtgttaag actaaaggag ataaatgtca ccgcaggatt cattttattt
    15361 tagatgaatt tgggaatatg ccaccaatgg ataatatgga aggaatcatg acggtcacag
    15421 ctggccgaaa catgttgtgg gatctgttta ttcaatctta tcagcaactt tatgcgaagt
    15481 atggagaaca aaacgcagcg attatcaaag aaaattgtca gacacatatt tatattatgt
    15541 cgacagatga taataccatc gaagaatttt ctaaaaaagt ggggaataag acggttgaac
    15601 aagagaacag taccatcaat tcattaggta tgaatactca tatcaatcgc aatgttgatt
    15661 cagatcgtat cttaactcct gaaagaattt ctactttttt agaaggagaa acgattgtac
    15721 tcgctccttt aaaacgacaa gatttgaaag gtcataaaat acggccgtat cctattttta
    15781 atcatgggga aacgaatctt ccttatgctt atcaatatat tgaagatttc aatccatcgt
    15841 gggatatcaa tgatatagac atacattgta cacatgcgga cttggatctt aaggctttac
    15901 aaattgatta tgattatttc ctaccagatg aatatgctag aaatctctat catggactgc
    15961 atgatcaatt agaagaaaaa aacgaagaaa atatgtttta taaaatcgtt caatcttttg
    16021 agaatgagac ggctaaaaaa ttgttaattc aattttacga agaacaagca gcagatcgac
    16081 ttcaagaata tgttgaaaag ttgatcaaaa ataaagaaat ttctgaaatg attgggaaac
    16141 ggttgttatt ggaatctgaa aaggtggtga aagaatgatc attggaggaa ttttttctgg
    16201 catagccgac tggtggtcag gcttattcgg agaaggggat gcaaacccca aaaccgtaac
    16261 gaaagtctta caagattatg ggaattatct gcagtatcaa gatgtattta gttggatttg
    16321 gaacactttt ctgtggggat tgattaaagg attatataat ctcaatactt tactagaaaa
    16381 aaccatttat caaagctttg atttaaaaga cttattgaac gccgctgggg taaatgcact
    16441 atttcaaagt ttgattagta aggtattggc tcttttttgt gtcgtcacat taatttattt
    16501 aggcttgaaa ttttttatga gtaaacaccc accaaaagtc aaaaatatct tggttaattt
    16561 atttatggcc atgatcttga tttttggtgg tagttcttta atcgatgaag ggttaaatat
    16621 cagtaaaagc ttttacggag acgtcactgc agcaaataaa gcagataaaa cgggatcacc
    16681 tgcttttcaa attattcaaa ataatgtgat cgatgtcagt acgttactag caactgaccc
    16741 gaataaagta gaatccctac caacagataa aagaaatgct ttaacagcga aaaattttaa
    16801 aacagcgaat atcaatggga ttattactcc tgaacaagct aaaggtatgg caggtaatga
    16861 taaaagtggg ttatcagata tacaaaagga tcggttaaag gcattacaat accgattgga
    16921 actagatgat aatgggaaag aaattcctgt tcaaattagt gacgaaggct tagcaaaata
    16981 tgtctatact tcagggtatc gtcgatacag tagtaaacca ggtattattt tagctggtga
    17041 atcttctttg gcagtagcct atttatttat cctatttacg atcgttactt gcattattga
    17101 gctggtattt aaaaaattct atttagtgat tgccgcgtca acggactttg agacaggtca
    17161 acggatgaaa agtgcgatag aagatatttc tcaatccttc ttgttattgg cgtttacgat
    17221 tttagaatta cgaatctata ccttaatgct ttcaggtatc ggtgatttgc atgcggcagg
    17281 aaagatcaat ggattcttgt atgtcgttgc tttgattgta ctaaccattg cgctattcaa
    17341 aggctctcaa tcagttacga aaattttcgg cgttgataca agcctgaaaa acggcagtaa
    17401 ttctcttctt agcatgtttg ccttaggaaa tattgcgaaa aatgtgggta gcggagctaa
    17461 aaaccttgca agtgctggta agagtggttt aagccattta aatgatattc gtaaaaatgg
    17521 atttggccat cgaaaagatg ttacccctga taacggggaa gaacaatcag acgataaagc
    17581 tacaactgta cctaatagta agaaaacagt ttccgaaatg ttcgggaaag cgaaaaatgg
    17641 tgtttctaaa gccgcagaag gtcttggtta cgtcaatgaa cgtgggttaa aaggaacagc
    17701 tgaagatctt acagaaaaag ccaaagacac cttagaagat aaattagacg gcacgagagc
    17761 taaagaattg gctgaagctg tgaagaatcc atccgaagca ctagaaaaac aaaaagaaaa
    17821 agcttcagaa gtcaaagaag gactaaaaga tgcgtatggt gaaggacaag tcaatgcagc
    17881 agtgaaagct aatacaacaa ccgaaaaaga aaatcaagaa ggtatagtga ttccttcagt
    17941 caaagatcac caagaagcgt tgcactctaa tgaaaattcg attgaagaga aaaattccat
    18001 agaagaaaga aattcagaag aaggaaaaaa tggacagtta ccaaaaactg atccagaaca
    18061 atatcgctat aaacctaaca gtgaccttga aaatgagaag cgaatttcag atttatcaaa
    18121 agtaaatcaa gaagacaaag cagaaaatga gggacagtta ccaaaaactg atccagaaca
    18181 atatcggtat agatctgtta actccgaaaa tgaaaaacgc atttcagatt taccaaaacg
    18241 aaatcaagaa gtacaaacgg atgaggattc catcgttctg ccaaatgata atggaatcga
    18301 agttggaact gaaaaatcaa cgaaaattgg tttacctaag gcacaaaatt taccaatgaa
    18361 agatagtcgt ttgaatcaat tcaaggataa tgatcaaagt tttgttcatc aatcacctgc
    18421 aggtactgtt caaatgaaac caagtcgttt gcctgaaaat ggaaatctga caactgtttc
    18481 ttacaattta tctgaaagtg gtgagacagg gaatgcgtcc gttcaaaatg ttgtgacaag
    18541 agaagctgca ccaaaaccta ttcaaaacgt tcatacaggt tacaagccag caaatgtggc
    18601 atttactgca ccaacaaaaa taaatgtgac aagagaaaca aaaaacagga acaatgcatt
    18661 gaaagaacaa ctattgaggg atatcaaaca ggatatcaaa ccataaaagg cctacaagtt
    18721 aggccttttt tctatgtatg aaaaggagaa atcatttatg gataaatata gtaacatgaa
    18781 aaaccctcca agatttagtg gagaatggaa gtttttcgct tttttctttt tagatctatt
    18841 aacgcttggt ggctgctttt tttgggcgaa aatggtcaat aatcttgtcc acttaccact
    18901 cttttttgca ctcttgaatt atggcttatg gattgcgata gcgttagtga ttgtttggcg
    18961 accctataat aacccaggtc aacgacaaat ttttgcgatt atcgagtcta ttttttttgc
    19021 ggacaagaac cattatcatg cgttagatcc taaccgaaat tatcatggta cgtataagga
    19081 ggatgaataa atgcgagtca aaggacaaaa ttcagttgaa aggaaatcca ttttgcagtt
    19141 tggaaaacca gaagagcgct ctatcttgga cttatttcca tataaatcct gtacagaaga
    19201 gggagtctta attactagag aagatcattt tcaacgttat tatcgtgtag tttctacaga
    19261 cgtggaagga ttaaatgaac aagagaaact agaacgaatg aatcagttga ccaccgtcat
    19321 gcgtacgtat gtgccaacaa ttaagttagt tagtttgacc acagagacgg atctaacgga
    19381 acaaatcgtg caaaagcggc aattattaga taaaaatcgt atggaacagt ccttaggaag
    19441 aaatctacaa cgattaaaaa aatatgaaaa gaaattagtc gaagaaatcc aggaattgaa
    19501 acgagcagaa caagaacgtc ctgacttaag cttctttttc gtcattgaag gaaagacaat
    19561 caaggattta caaaccagaa accgtcagtt aatgcgatct agtggcattt tagggttaaa
    19621 accactctac aaaaaagaat tgatcaagat tctgtatcga atgaacaata tgaatgatga
    19681 gtaggaatgg agaagagaga atgaaatatc cgttgatatg gttagaaaaa gatgttccga
    19741 aggaagagac cactagaaaa tttcctcaca ctttaagcct gggtaagttg ggaaaagggc
    19801 aatcttttaa ccaagtaaag aatcaaacaa gaagaggtga ctgtacatgt tagaagcaat
    19861 taaaacagct ttttcaccac aaaatgatca aaaagtactt gatccattaa agcaactagg
    19921 aaatcgggaa atagaaattg taaaatccaa agggtatgat ttagattttt taaaacaagt
    19981 gatgccacaa ggtgggttaa attttgatga agaatatgtg acgtatggta atggttattc
    20041 acgatgtgtg acagtttatc actacacaaa aaatcctacg cttttttggt tggccaatct
    20101 catgaacaat cctcatacag tggccacact cgatgttagc acagatgaaa agcaaaaagt
    20161 gattgatcaa atcaatcgtg ccttagatga attaaatgat cgatcagata atgaacgaaa
    20221 agcgactgac cgaaataaaa gcgcggaaga atacctggaa ctagcggaat atgctagaaa
    20281 attgacttct aatggggaaa tttctaagca agtcaaagtg agagtacatg tttacggaaa
    20341 cacgattcaa gaagtagatg aacgagcgca ggaactgatc gaaaatctca aagggatgga
    20401 ttatcgtgcg gtgacttatc tgcataaaac caaacgagat tggcaatcga tgtttaccat
    20461 gttcgatgaa caagctgaat ggttcggaaa tatcaaaggg caatcactac catcgttaaa
    20521 cattggcgga ggtttcccgt tccaccacca aacgctaaaa gatccaggag ggacacaata
    20581 tggggaaacc atgacaggcg gcccatttgt ctggaatgcg acgtataaaa atagtgtacg
    20641 gttgtctact aataccttgc ttctaggaat gatgggagct ggtaaatcga ccgttcttaa
    20701 aatgatcgcg gaagcccatt tagctgcagg agactttatt tggggctttg aaaagggaaa
    20761 agactttatt cctttcttaa aggaatataa cggtattatg gtgcgtttag acggatctga
    20821 cggaatgatc aatcctttag agatatttgc cacacggacg tatgatgaag catccagttt
    20881 atatgacgat ggatcagtca aagatttgaa gatcaatgaa gcagcgtcct atcaaacaca
    20941 cttagacaaa gtggtctatc aggtacagtt agtgagtcct cagctaaaag gaacgatgaa
    21001 agcagaattt aagacctact taaatcgttt ttatgaagag tatggaacag taccacgcgg
    21061 ctttacttcc tcaaattcgc gttcaaatac tgatacgcaa gtcacaggga aagatccaga
    21121 agcttatccg acgttcaaag aatttttaga ctttttagga caactggaat taccaggtgc
    21181 ttcccaagag aaaaagaatc gaaaagaaga aatggaatcc atcgtagaat ccttatgtga
    21241 gacgtatggc atgatttttg atggtcatag tacgattcga caccttgacc aacaacagtt
    21301 ggtctttttt gacattgact ctattacagg cttagatcga ggggtacaac aatgtcaaat
    21361 gtacatggct atgacgttga tttggaatca ggcattgatt caaggaagaa gacaacgacg
    21421 tttaattaaa gaaaaacaaa tggaagaaca agacttcaaa cgcttcttgg cgatgattga
    21481 tgagtgccat aatggtgcga cctgttctgc agtgaaaagc tgtgggttgc aagtagtcta
    21541 aaaactattg taataactct tataacgaga agtcaaatga gtgggtaacg ccacaaaggg
    21601 cttccactga tactccgaca tgcaacaagt agtttatgca gaccaacgtt gtgtgaagct
    21661 cggtgaagtc ggctgaagga taccgtaagg taaaagaaat agtgaatacc acgaaagttc
    21721 tctagaggta aagggtgtat ccgataagtc gggtgtccaa aaaaacttaa tatggtgagt
    21781 atgtacaaac gtactgacga actagcgaat gtactgtccg aaaggcatag ctcctagaaa
    21841 tgggagagac agtgagcaaa aaatcactca ttgtgctaac ttgtggttga gtaaaagtcg
    21901 ttgttatgaa agaccatgct gcgttacagg cgtaggagaa gttagggggc aaaagctagc
    21961 atctaagtta aggcatgtgg atagatataa gggaacaggg gaagccatca acatggagag
    22021 tgcatactcg atgaagtggt agtaagaaat atgtgaatga caaataactt actttagtga
    22081 tggtgaaagt agagtcacga ctgatgaagg ttctgtaatg gaactggagg aacaggctcg
    22141 attcgtctgt aaaagtcatg aaacccgttc aaccatggga ttctcgtatg actaaaaagg
    22201 tcacgagcca attggagcgt gataaaccat gacaaaagaa acaagtccaa aagataggaa
    22261 gttgcggcat gcagaatatt atggcatgac ggaaatcttc gataagatat actccaaaag
    22321 tttgaaggga gataatttca agaatctaat gcagattatc atttctgata acaacattgt
    22381 cctagcatat cgtaatatca agagaaatca cggtagtgtg acaagaggag aagatggagt
    22441 aacgattaaa gatattgaaa atctcaaccg taatgaattc gtagaaattg ttaggaagcg
    22501 attcgcacat tataaccctc ggaaagtaag gcgtgtagaa attcctaaat ataatggtaa
    22561 attaagaccg ttgggtattc catcaatatg ggatagaatc gcgcagcaat gcatcttaca
    22621 agtattagaa ccaatatgtg aagcaaaatt caataatcac agctatggtt tccgcccaaa
    22681 tagatcagca gaacacgcaa tagcagattg tatggcccga atgaatcaaa gtcacatgga
    22741 atatgtggta gatgtagata tcaaaggttt cttcgacgaa gtcaatcata ctaaactcat
    22801 gagacaaatc tggtctatgg gtatacgaga caaacagctt ctcttaatca ttaggaaaat
    22861 gttaagagca cccgtagtac ttccaaatgg gaagttgata tacccaacaa aaggaacacc
    22921 acaaggcgga atacttagcc cattacttgc gaacatcaat ctcaacgaat ttgactggtg
    22981 gatagcaaat cagtgggagg aaaaagatgc caaagagcta aaacatcatt ttaggaaaaa
    23041 tggagcttgg gataaaggta atatctatca caagctcaga aagagtacaa ctctaaaaga
    23101 aatataccta gtgagatacg cagatgattt taaaattttc tgtagaaatt ataatgaagc
    23161 gaaaaaagtt ttctatgcaa ctaaaatgtg gttacaggag cgtctaaagt taccaatatc
    23221 tgaagaaaag tcaaaaatca caaatctttc aaaagaagaa agtgaatttt taggctttac
    23281 gatgaaatta gtgaccaaag gtaacaaaaa ggttgttaat acgcatatag gtacaaaagc
    23341 acaaagcatg attaaagtga aactaaaaaa acaaatcaaa cgaattcaaa aaagtcctaa
    23401 tagcaaagat acctttatct atattggagt ctataacagt atggttattg ggattcataa
    23461 ttacttcaaa attgcgactc attgtagtgt agcaatgcga aaaattgcaa gagaactcag
    23521 accaagtttt gaaatacgac tacgagaaca aggaatctca aaagaaggac aatacggaaa
    23581 gtacgaagga tacaagcctt acctacggtc aggacaaata cgatatataa gagggtttcc
    23641 aatattacca gtagggtata ttcaacacag acatccgatg tgtaaaaaaa gaaatattaa
    23701 taaatacacc gaagaaggta gggaagccat tcataagaag caacaaaccg tagaagaatg
    23761 gaagatatcg tggttgtgta gcaatccagt gcttagtaag agagcaacga ttgaatttgc
    23821 ggacaatcga atttcaaaat tcatcgctca aaagggaaga tgtgcagtaa caggtgagga
    23881 gcttatctta tctgaaatgc attgtcatca tattatccct tatcatgaaa gcaaaagtga
    23941 tagctatgaa aacctagtga ttgttactga ggaggttcat cgggtaatac acgctacaca
    24001 aagtgaaacg attgaggaac ttctaaaata tctaaaatta aatccaaaac aaaaagaaaa
    24061 actcaatgag ttgagattaa aagttggtaa cgaagaaata tcttaataaa tatttgtagg
    24121 acgggaaaac agaatgtctt ttatagacga gggaacgccg tatgcggtga aagtcgcacg
    24181 tacggtgtgg actgggggaa aagacggaga taatatcaaa ctcttaccta tcagtatcga
    24241 ttaatccaga ttttattgaa acggtaaatt atgtcacaaa ctttcaacga gaaatgcgta
    24301 aagtagaagc aatgactgtt ttagctacac aatccccaca agaaatggtg ccagaaaata
    24361 tgtcttctga taacttctcg aaactgaaga aagtctttga attttcttct acgaagatct
    24421 ttatgcgtat ggatgaatct attttacgtc atatagaagg attagtgggt aaatcaatga
    24481 cgaactcaga attagaatcc attcctcaac aaaatcaagg agacgctgtg atcaattttg
    24541 gctcaaaaga aaccattcgt gttcatttta gtccgaacaa acgtcaatta aaattgtttg
    24601 atggaggaaa ataatggaaa accaaaatga aagtttaatc aagcagtatg ttaagcgtcg
    24661 tgcaaagcgg cgcttatttt tgtggttgtt tggtacttct gcaggtttga ttactatttt
    24721 gatcactgtg ttcgtgaccc tatttctaat acttgcggcg ggctcgattg ataattcgga
    24781 ttcagattca agcagtggtg gcgaagcttt tacaggagaa tattcggaag gcttaccgat
    24841 ttataaagaa atcaaaggaa gaggcccttt ttctgatgag attgcgcaat atgccgtagg
    24901 tgcagcagtg aaatataaat tattaccaag tgtgatcctt tcgcaatacg gttacgaatc
    24961 agcgtttggt acatcggcgt ccgcaagaaa tgatctcaac tattttggga ttacttggtt
    25021 tgatggttgt ctgtttccta aaggaactgc acgaggaatt ggtggcattg aaggtggctg
    25081 gtacatgaag tttcccaact caaaagctgc attttcctat tacggcttta tggtagccac
    25141 acaatccaat tttaatgctt gtgtaggaaa taaaagccct ggcgcaagct tattgatctt
    25201 aggacgaggc ggttatgccg ctgcaggaat tacggaagat agtccgtatt atacaggttg
    25261 tatgtcaatt atcacttcta ataaattaac cgaatatgat gaattcgcca tcaaacattg
    25321 gggtgaaggt ggcaataaca acggcacgat tactggtgaa tggacgaatc cattccctgg
    25381 cagcagttta gataaaagtt ccttcagcgg tggtcaatta tttggaacga atccaggcgg
    25441 tgaattccgt ccgaacggct tccatgatgg attagatttt ggttcagttg atcatccagg
    25501 aagtgagatt catgcggttc atggcggaaa agtcgtgtac gttggcaatc ctggtatttc
    25561 aggtttgggt gcgtgtgtga tcgtgattaa ttacgatggc ttgaatatgg tgtatcaaga
    25621 atttgcaaat agtacaggaa attctcgagt gaaagtaggc gatcaagtga aagttggtca
    25681 agtaattggg atacgagata cagcacactt gcatttagga tttactcgaa tggattggcg
    25741 tcaagcacaa ggacatgcat tcattgatga tggtacatgg atcgatccgt taccattttt
    25801 gaatagcagt aaaaaataga tcaggagaaa aaatatggaa aataaaacgt taaaaatcgt
    25861 cttaacagct gcaattagcg ttagtttaat cggcaatatt gcgttgtttt ggcagctgaa
    25921 agaggaacaa gcagcaaata cctataaaat tgaagaagtg gctaaaaacg cgtctgagaa
    25981 gctcaaaaaa aattcctctg aggcacaggc gaagatcgac aagttaaatc aaaccatcaa
    26041 tgagaaaaac caagaaatcg atcagctgaa gcaacaagat ggcacaaaag caaaagagga
    26101 agtaactgaa gcacaaagaa agacggcaga agagttcggt caattggcca ttgataaatc
    26161 attaagcaga aatgaattaa cggaaaaact aaaagatgtc gcaacccaag aagtgatcga
    26221 caaattaagt cctacagacg atgatcacca acatgatgat tacggaagtt actcctttac
    26281 attaggtgac gtgcaaactt acgctcaaac aagcagtgta aaagaggaac aaaattttgt
    26341 cgtatttgtt gattatacaa tcggcaatcc gcaattcaaa gatgtcaaac cacaaaaaat
    26401 aaaaggtgga ttaactgtca ctgaaaaaca agtagatggt caatggaaag tcatagactt
    26461 tagttatttc actcgttagg aggaagaaaa tgagtaagaa aaaaggagaa agacaacgtt
    26521 tcaaaaaacg aaaaagttat cttagatggc tgggaatctt tatgattctc ggctttttga
    26581 ttcttgctag tccgatttat acgaatcaga aggtagatat accagatgag gtaagcacga
    26641 acgccttttt aaacacgaaa gagctgacaa tggtttcaaa agaaaaagga gcgaaccagc
    26701 aattagtttt tgaatttgcg atcgatgatg aagatcaaag cttgttaaaa gaactggcta
    26761 atcttaatta tcgcgtagaa gtaaagacag ctaaagggga ttatcaagca atacgagcca
    26821 aagtgattaa ggtgtctgat gattattttg cagtgaagct aacgcatgta ccagaagaat
    26881 atatcgccat gcgtttaacc attattcctg aaaaaattga tccaaaagtg gatatgcaag
    26941 aaccacaaga tctgattttt tatattcacg aagataaggt caaagatcac gtgaaagacg
    27001 aagattatga acaacatgcg atcaattaca aagtgaaagg atacaagaaa gaacaaaaag
    27061 cagctcaaaa acaaatggaa tctttccaag caaccattga tttgaatgaa gaattagtga
    27121 agaagctaaa agatcaattg ccttatcaag tcgcagatga tcaggaaaat actgaaaaca
    27181 agattaatag ctaccagcaa gaaattgaaa catcgaaaac gcaactgaaa gatcaagaag
    27241 agatcattca aaaacttcaa gaaaaaattg atcgtatgac aaaagaaaag atttagaagg
    27301 acaggtggag tgaaaaaatg aagcgaacaa acttggctga caaagatact ttcgctgagc
    27361 agaaagtatt gattaaagcc tatctcaaag ttgtaaaagc taaagaaata tgtcgagaag
    27421 tgaatttatc taacacaaca atatctgagt tgaagaatgg tcgaaagaat ttagatacct
    27481 taaaattcag atcatttgaa gctctatatg tagctgctaa aaaatatttt acagctgata
    27541 acaaataaaa aacaggagta ttttatgtga aaaaagtata taaaaaatgc ttatgaatca
    27601 gctatatcac cttaaccatt tgttaatgag atgggagaaa acattaacca cgattctaac
    27661 gcaaacatca agcacataaa acatttaaca ctcctccttt ctggctgaag atttcaacca
    27721 aacaggaaga cagcgtatca attatagcat acactttttc ttaaattcat ttttcatatg
    27781 acaaaatatt tccttctaat cgacaaagtt agaaggattt tttattgtct aaatacacaa
    27841 ataaccttaa tttttaaaga gcataaggaa acgtgatatc actttctcac gaaaggtgtt
    27901 aggtaggctg tgcctaccac gtttctaaga aagataaatg atatcaaaat tttggatttg
    27961 tgtgtagatt tgtgggtagg atgaaagcga atgtgggcag taaaataagc gtttgtgtgt
    28021 agcaaaatca aaaaatgtgt agggatatgt gtagctacac atatccctac acataataaa
    28081 tttggaggaa aatatggact tattaattag aaatattgaa gaaaagtata ttaataaaat
    28141 tgacaaacgt tgtgaagaac ttacggtcaa gactggcaaa aaatggagtc gaaatcaata
    28201 cctaaaagtt ttgatcgaaa atgattttga tcacgctctt ctgaattata aaaaagacca
    28261 atttgatcgg ttgttagaaa aatttgtgga tattcaaact tataatacga aaactttaga
    28321 agaatatatt gcaacaaata atcaattaat tggtttgtta attgaataat aaaaatagta
    28381 ttgtgaattt ataaaaggag gaaatcaatt ttggcaaaag caacaagaaa acatgtatat
    28441 ctttacgata tgcatatcga agtacttgat gaaataattt ctactcatga agatatatct
    28501 aattatagtg aagcagtcag acatttgtgt atgatgtatg atccaaaaat cgaagaaaaa
    28561 ataaatccaa ttcaaattaa attgaatgca atgggaaaag aaatttctat tttaaccgaa
    28621 atggtttcag agtttgcgga tcttcatttg aaagatacag gaatactaat tggtacaaat
    28681 tcaactgttt atcaagaagc agaagaacgt gtggaaggaa aaattaaatc caatcaaacc
    28741 aaaaaatatt cttctaagtt aaagaaacca gtgaatgaat ctgttcatac cagagtagat
    28801 ccatttgatc caacgaagat tctaaaaaga taaaagtaga aggagaacgt gatgaataaa
    28861 tcgcctgcag ttattcttac ttgtcaattt accttgccca ataaaaaatc tttttcaaca
    28921 tacattgatt atatgacaag agaaaaagca ctggaagaaa tggaacgacg tacgcccgaa
    28981 gaagaacagg aactgcaacg aatcaagaat gctttagagg atttttatat tcctgaagga
    29041 gaaacttttt cggaaagtaa gacggataaa gaattcagtg aaacgtcgaa tgaagctcaa
    29101 tcgttgatgg aacatggaat caattttgac aagttagagg aacaagattt taccaaatat
    29161 ctttcttata tgacccgtca ctatgcgtta gaacaaaaga aagaactgac gcaaagggaa
    29221 caaaaagaat accaacggtt gaaccaagca atgaaaaaat atcaaacaga gccacataaa
    29281 aaagcttcag gaaatcttcc tggagttttt tctatggctt cagacgaagt gaaaggaagt
    29341 gatctgaagg agatcaaaca aatcttccaa agaggacaac aaaaaggctc aatcatttat
    29401 caagatgtta tctctcacga caattcgtat cttgaaaaaa tgggcttgta cgatcccaaa
    29461 acagatacct tagatgaaga aggtttgaaa gcagctggaa gaaaaatgat ggagacatta
    29521 ttcgagaaag aacaattgaa tgaaacaggt tactggatgg caacgattca ccgtaacact
    29581 aagcatatcc atattcattt tgcagttgtt gaaaaagaaa atacgcgtaa accgtatact
    29641 gagattgaaa atggtgtgag ttatatcgta ccgaagggga agagaagaca agcaacatta
    29701 gacgcaatga aaaccaatta tgtacatgag ttagaacgtt tctcttatga aaaggatcga
    29761 atgatcggta tggagaaacg aaatttattg acacgaaaaa gtgatttgcg taacacctta
    29821 acaaaagtag taaaagaacc agcacgttac gatcgacacg ccatgaactt attaaatgaa
    29881 gtatataaaa ccctacctga aaaacgttcg gaatggaatt atggagatga aaatcgaacc
    29941 aagctttcac catttacaag gggaaaactt gatgagttaa ccaaatatgt tttaacaaaa
    30001 gaacctggat atcaagaata tatacagatt tcaaaagcat tgaaagaaga agccaaaagc
    30061 ctttatgggg aaagcaaaag agaaacaaag aatgcagaaa aaaacgccct ctttgattta
    30121 aaaaagcgca caggaaatgc gattttaaca gaactgaaga gacaggatag tcaaataaaa
    30181 ccattaatag aaagtgtcaa tcagatgcaa tctatttaca atgaacgatc ctttcgaaca
    30241 ctcaaacagt tgccaacaaa tgaatacaag tatagttatc aagaattcgt aaagcgtagg
    30301 aaacaattcc aacaaagagt tgtcacgcag cgaagcttgc gtcaattaaa tcgaacaatt
    30361 aatgcacatg aagaaaaata ccacgcaagt aaagcatatg aagagcaaca gcgaagaatc
    30421 caaatggaac aagaaagaca ataggaggaa gagaaatgaa acaatattta gatcaatgga
    30481 aagtcataga aggatcacta aaagaagaac gaatcgaaca acttcctgat tgtttagaaa
    30541 aagagcattt gtttcagata agcgaaatgt tgagaaacga acagtttgat ccaaatcaat
    30601 ttttagttgt tgagtaccca gctactggtg tgtattgttg taatcatgtg aagggtgaaa
    30661 aatattttat tattcaagaa tacgaaggta agttagcgcc ttattatact acttgggaaa
    30721 taaatgaaga gggaatcaat aatttcccat gtgaaagcat tgaagagtct atttctctca
    30781 cggaatgtta aataaataga gacgtaaaag agtcctgaac gtgattgtcc aggactcttt
    30841 ttcaatgaat tgttttccgc caattcactg agtggtgcga ttcaatttta ttatatcaaa
    30901 agaaagtgag attccaaagt gaagggagga agaagaaatg actcagacaa cgattggtgt
    30961 tgtggttgaa aaaagcaggg ataatctcgt ttttgtgaca gaaattcaaa caggtagagc
    31021 ttttgtagtc acagacaaag ctgcaaaagc gtatcaaaat ggcaatattt taacgttaaa
    31081 tctgactacg aagatgttcg ttgatgcagt agaaaattat ccatttgttt agacaaacga
    31141 tattttacaa ggaggaaacg gacatggcag aagaaaaaga accactagat aatagtcggt
    31201 tgggaaagtc caaaagaaaa ttagtacggt tgcaaaacga attaaataat caatttcaaa
    31261 agctatttga tcaccaaaaa ttgacaaatg ggcaaccaat gaatgacaaa cgaaatggtc
    31321 gttcatggtt taaccagcaa gaaaaaatag aaaataaaat acaaagtttg agagaagaga
    31381 tcaagcaaca agaaaaacag gtagaaaaac ttgaaagaca agaagagcta aaggaaatgg
    31441 gctataacaa gtacggcgga ctggatatga cgatcgataa tattccaaga atcaaagaag
    31501 aaatcgaacg ctttgaaaaa ggtgaatcga ctttttcagc cgccacgatc cgaaaatatc
    31561 aacggaagtt ggaaacatta gaacagctga aagaaaaatc agaaaaagga aaagaaaata
    31621 tcctccctga agtacaagcg atcattgaca gcggacgagt gacgcaatgg aaaaaaaatc
    31681 caaccattta ttttttgaaa ggctatcgaa aagttgcttt agaactgaat gaaaacgggg
    31741 gatttgagga atcaaaaaaa taccaagcca cgtcggaaga ggaaaaagca gctgttcgtg
    31801 aattacttga agaggctgca ccaactgatt ctctgaagga gaaagaaaaa gatcatttct
    31861 atcgagtaga attcaacgaa aatgatccag aaaaaggatt gaaaagttat ggtggggaga
    31921 tcgtaacatt agaattgatc gatcaactta aaacgttgga taaccaatac gctgcagaag
    31981 caggttatta taaattctat tttgaagaag tgaagaacgg agaagtagta gatcagatcc
    32041 gaatggatct aggggatggc gtcaacagta atcaccccat ctatttagag ttagcaaaat
    32101 tgtgcgaaca agtacctgag aaggaacaaa aacaatcggt caaaaatgaa cctgtttcga
    32161 aagaacaaat gcagtcgaaa gaaacaaaaa caaaacaaaa gggctatcaa aaacgaacgg
    32221 cagaagagat caaacaagaa gttaaatccc tttcgcaaca ggcactggat gcggtgaaaa
    32281 agcataccca ttcttctgaa gacgtcaaag aacttttgaa ctttatgtct cgttttccag
    32341 aacgatcctt tagaaatcaa ttattgattg aggaacaatt tccaggtgca acggcttgtt
    32401 taggtcgtgc cgcgttgaaa aaagaaggaa tctatatcaa aaaaggggaa caagggaaca
    32461 agatctttgt cagaaaaacg gtcaaaggat tttatgagaa aggacgtggc tttgttcgag
    32521 aaacagaagc tacaccagaa gataaaaaac gaatcgaaag tggtcaaatc gaagtgatca
    32581 aaaaacctta ctacacgatt gaaaaagtgt ttgatatcac acaaacacag ttaaaacctg
    32641 aagactatcc gaaaatcttt ccgaaccgtg tctttgattt tcaattggat aaaaaaggac
    32701 agtcagaact tcaagctggg attcaagcgg tagccaacaa tattggaata caaattcgag
    32761 atatgacaga aagtgaagtc tatcaaagag aattaggaca agcacgtggg gcgtatgttc
    32821 gtaacgaatt tactggcgaa gaagaaatcg tcatgaatac tcgaaatact cctacgcagc
    32881 atttagctac aagtattcat gaattagccc atgcaagaat gcacaaattt tcagaattag
    32941 ataccgcaac taaagaattg caggcagaaa tgacttctta tattgtgtgc aaacattttg
    33001 gaatggacac ttcggagaaa gctattcctt atattgcagc atggacgaag aatggtcaga
    33061 cgttggatga taaggaagcg gcggaacgtg ggaaaatcat gaatgatgtc agccgtgtcg
    33121 caaatgaatt cattcaaacc atttctaatg aaatcaatca atacagagaa agagaaccag
    33181 agatccaacc agcagaagtg aagcaggagc ataaaaatac tgtagaaacg gcagagaata
    33241 agaatattgt gcccgttggt tcactttgga tcgacaagaa agacgggaaa gtaatggaag
    33301 aagatacggg tcgaacgtta catttgaagg atgcggcgtt tattgaagat ccgcagcata
    33361 atcaagtgat tttaaaagga aaaagttatg gaaaagagat tcaaacggcg ttaccttcag
    33421 aagaatttgc gctaggaaat ccattccgat tttcacttga aacagtgtca gaagtggcca
    33481 aacgaaaaaa tgaaatctat tatgaacaac aattgtcaca aaaagaacgc gtataaaaca
    33541 agtaaaaaag actatctgaa aaacggatag tcttttttct tttgggaggt ttgaaaatga
    33601 ccgaaaaaaa aatgagtctt attgatcgat gtaaacagat cgatatagtg gattttgctc
    33661 gaaacaatgg aatggctgta gtcaataaag gacgagacta tcgcttagaa gaccatgatt
    33721 catttgtttt tgatcgaaga aaacaacgtt tttattggaa cagtcaaaat attagtggcg
    33781 acattattga attagctaaa ctttttttca tagacaaaga gatccaagac tctaagcaac
    33841 aatttaaagc agccactgat tttattctaa agaatgaaga caaaacagaa cgagtggaaa
    33901 atcttcattt tgaaacagaa aagtacaaag atcaccccgt tgattatcaa ccactcacag
    33961 aaaaaggaag gaattatctg aaggaagaac gaaaactacc agagtggctg attgattatg
    34021 cagaaaaaga agggttgatt gccgaactaa agccaaaata tgaacggcaa aatttcttag
    34081 ttcgagacga tcgtttagat catgcggtcg cctttttatg gaaagatcca cagacaaaag
    34141 agacagtagg ggcgagttat caaggaacgt tcattgatta tgaacgtttt ggtgagagag
    34201 gcacatataa gcatatagat aaaaattcga cagcgaatca tggctttaac ctaaagatcg
    34261 gtgaccctaa gcagttaaaa ttttttgaga gtagtattga tttacttagc tatgctgcac
    34321 tgaatcgtga tcagttaaac gatacgtggt tagtctctat ggaagggtta aaacaccatg
    34381 tgatcagtca ttattttggt gaggcagtat ctgaattgag aaagaaacaa gcctttcctc
    34441 aatccatcga gatttgtgtg gataatgacc gtgcaggaca tattttctac gaaaaagaac
    34501 aattaatggg cgcagtcgat ccatttacga atcagaaagt acgttgtgaa cgaggaatcg
    34561 ctaatgattg gcaagtgcca aaagaataca aaatcattta tgaagaagtc gcaaaagaag
    34621 aaaaggtcac accagaagct attatggcca tacacaagac agaaaacaat ctgcaattaa
    34681 cgaaccaact ggtttccgct cacaaagtaa atgcgttttt tggccagcag ctttctgtta
    34741 acgattcaat agaagccatt aatttgaaag atatttgtag agaagtcgca aaagaattaa
    34801 aagtttgtga acgcgtggat ggtacgtacg attttgatcg tttttatcaa gaaaaagggg
    34861 atattaatgc gcagattctt ttctcttata aagcagaaca atactacaaa ggctataaaa
    34921 atcatgaaca tgaatttata cctgaagtaa aaaaggactg gaacgatcaa ttgaagcatg
    34981 agatccagca acaagaaatc agaaagcaaa aacgtgccat gttgtttcaa caaggcagac
    35041 aacaagaaag ggagtgaaat cgtcatgatg aaaaccgtta ttttagcaga aaagccgtca
    35101 caagcgaaag cttatgccga tagcttttct aaagctaccc gaaaagatgg gtattttgag
    35161 atccaagatc gattgtttac aggagaaact gtgatcacgt atggttttgg tcatttagtc
    35221 gagttagatt cgcccgatat gtacgatgaa aaatggaaac aatgggcatt agaacatttg
    35281 cctattttcc ccaatcaata ccactatcat gttcctaaag ataagaaaaa gcagtttagt
    35341 gtcgtcaaac gacagctgca gtcagctgat acgattgtga tcgcgactga tagcgatcgt
    35401 gaaggggagc tgattgcctg gtcaatcatt caacaagcag gcgccaatca aggaaaaacc
    35461 tttaaaagac tttggatcaa ctccttagaa aaagaagcca tttatcaggg attccaacag
    35521 ttacgagacg cagaagagac ctatccaaaa tttgaagaag tacaagcacg tcagatcgcc
    35581 gattggttaa ttgggatgaa cggtagccca ctgtatagtt tactcttgca gcaaaaaggc
    35641 ataccaggaa gtttttcttt aggtagagtc caaaccccaa cactctatat gatttatcaa
    35701 ttgcaggaaa agattagaaa cttccaaaaa gaaccatatt ttgaaggaaa agcccaagta
    35761 atcgctcaaa atggcgcatt tgatgcgaag cttgatccaa atgaaaccca agcgacacaa
    35821 gaagcatttg aagcctattt aaaagaaaaa ggtgttcaat taggaaaaca gccaggtacg
    35881 atccttcaag tcgaaacaga gaaaaaaagt gcggctagcc cacgtctttt ttctttatcg
    35941 agccttcagt ctaaaatgaa tcaactcatg aaagctagtg ccaaagatac tttggaagcc
    36001 atgcaagggt tgtatgaagg aaaatatcta agctatccgc gaacggatac cccttatatt
    36061 acggaagggg aatatgcgta tctgttggat cacttagacg aatacaagca ttttttaaaa
    36121 gccgaagcga ttccgactcc tattcataca ccgaatagtc ggtatgtgaa taataagaaa
    36181 gtccaggaac attacgcgat cattcctaca aaaacggtta tgacagcagc ggcttttgaa
    36241 caactaagtc cactgcagca ggctatttat gaacaagtgt taaaaacaac ggtggccatg
    36301 tttgcggaaa agtataccta tgaagaaaca acgatcttga cgcaagttca gcagcttcag
    36361 ttaaaggcaa tcgaaaaggt gccgctagat ctaggttgga agaagttatt tggaaaagag
    36421 agcgacggaa aagagaagga agaagagccg ctacttccta aagtgacaaa aggagaaatg
    36481 gtgacggtgg atctacaagt tcttgaaaaa gaaaccaagc caccacagcc ttataccgaa
    36541 ggtacgttga tcacggcgat gaaaacagct gggaaaaccg ttgatagtga agaagcgcaa
    36601 tccatattga aagaagtgga aggaatcggt acagaagcga cacgggcgaa tattattgaa
    36661 accttgaaac aaaaggaata tatcaaggta gagaaaaata agctcgtggt gacgaataaa
    36721 gggattttgc tttgccaagc cgtggaaaaa gagccgctgc ttacgagtgc cgaaatgacc
    36781 gccaaatggg aaagctactt gttaaagatc ggtgaacgaa aaggaacaca aaccactttt
    36841 ctagccaata tccaaaaatt tgtctctcac ttattggaag tcgtaccagg gcaaatccaa
    36901 tccactgatt ttggatcgac gttacaagaa gtgaaagcag ccagtgaaaa gcaagaagcc
    36961 gcgcgtcatt taggaatttg tccaaaatgc caggaacaag aagtcttact ctatcaaaag
    37021 gtggcagcat gtacgtctga agcctgtgat ttcaaactat ggacaacgat tgccaaaaag
    37081 aaacttacag cgacacagct gaaagagatc attcaaaata gccgaacaag ccaaccagtg
    37141 aaagggttaa aaggtcagaa gggttcgttt gaagcaacga ttgttttaaa agaagacttc
    37201 actacaggct ttgaattttc tgaaaagaaa aaaacgaact tcaagaaaag aacacgacgc
    37261 actacaaaat aatggagggt taatcttatg cgtaatacaa cacacaaaca acaagaaatc
    37321 gaaaatcaaa aagccaaaat tcgttcagaa caggaaaaaa tgcgtgccag caaacgaaga
    37381 ctgcagcaat tgaagaacca gcagcggatt caaaaagaaa tcgaattaga tcgataaatt
    37441 cctttcttgt taatataaaa ctaacatgtt atagttgtgt tagttttata ttaactttag
    37501 gggaagagta atgaaagaca aaatccaatg tatttttgat aaacataatg gcaacctatc
    37561 cttgaaggat gctagaagtg aaggattatc gcctatgacg ttagatcgat tagccgaaaa
    37621 ggataaaata gaaaaggtag caccaggttt ctatacctta aaggatgcgt tcgtggatga
    37681 attgtatctc gctcagagta tctattccag agggattttt tctcatgaga ctgcgctcga
    37741 tctttttcaa gtaggaacat atatccctaa aaagatcaat atgatgtttc ctaaaggata
    37801 caatgtatca aaggagaaat tagatcagta tgccattcaa ccgcattaca ctaataaaga
    37861 aaattttgac ctgggagtca cagaaacaga aagcttttac ggcaataaaa ttttagtcta
    37921 tgatttagag cgaacgttat gtgacatgtg gaatccaaga tacaaagcaa gcgtagaggt
    37981 aaaacaagaa gcattaaaag aatatatgac aagtaccagt agaaatacaa ataagctaag
    38041 aagatacatg gatattttaa aatcaccaaa agaaatgact ttgtatatgt tgccacttta
    38101 ttgagaggag taaagatgaa tgacaccgac acaatttaaa gagaaaacaa gaaatttcgc
    38161 agcagagaaa catttaaacc cacaaattgt ccaaagatat tatatgatgg aaaaattatt
    38221 agaatgtatt gttgaatcgc cttatcatga tgattttgta ttaaaagggg gatttctgat
    38281 tggatcaaaa tacgggttag aaaaccgttc aaccgttgat attgatacga cttatcgaaa
    38341 ttcgaaacta acaaaagaaa agttagaaac tgttttttct gaattaacag ctaatcctac
    38401 aaaagatgga attcgttttg aattaaaggg attaacagag acgagggaag ccgattatta
    38461 tccaggtttt caagcaaagt ttattgctta tcttgaaaat gcacgtgtac cttttaaatt
    38521 agatattact tcaggtgatt ctattattcc agcagcattg atgtatgaac acaaattaat
    38581 gtttgaagat aaaacgattc atattccagc ttatcctacg gaacaaattc tagctgaaaa
    38641 attacatgcc acgtttagtt ttggaaaaga taattcgcgc atgaaagatt tttatgacct
    38701 gtatatgatt ccgaaattag aacgtatcga tgagaaaaat ttgtataaga gtgttcaagg
    38761 aacattcaag gaaagaaata atcaatcggt gtttaagcat ttgtatgatc aggaaatgcc
    38821 tcaattggtg aaagatgagg gcttgagaga aaagtggaaa agataccaac aagaaaacta
    38881 ttttgccaaa gacatttctt tcgaatctac actaacttcc gttgattccc tgatgcaatc
    38941 aattgtagag caagagaagg cagaacgaaa acaaaatttt aagagatcac aacaaatgaa
    39001 tcaagaaatg gagagataaa gatgatcaat aatgtaactt tagtagggcg attaactaaa
    39061 gatccagatt tacgttatac cgcaagtggc acagcagtgg cgactttttc cctagccgtt
    39121 aatcgtaatt tcacgaatca aaacggcaat cgagaagctg attttatcaa ttgcgtgatt
    39181 tggcgcaaac cagcagaaac gatggcgaca ttagctaaaa aaggcagctt gatcggcgtt
    39241 gtcggacgaa ttcaaacacg gacttatgac aaccagcaag gacaacgtgt gtatgtgaca
    39301 gaagttgtgg ccgataactt ccaactatta gaaagtaaag cagcgacaga aagccgtatg
    39361 cacgctgatc aatcgtccag cagtccaagc cctaccacct ttgaacaacg ggatacagcg
    39421 acgcctaaca acaatggatt gaacgcttca cagaatccat ttggtggaca atcaatcgat
    39481 attagcgatg atgatctacc gttctaagta gcattttcta ttggttgcat ttgctacggt
    39541 ttatgttata atttagacaa caaaaagaat tttcaatcta taaccaaaaa ccactagctg
    39601 gcgggctagt ggctttcttt tacgtttcta ttataagaat taacgggtag gcttactgtc
    39661 tttcttggca aatttacaat caagccattt gcagatatag tgcgcggcta cttctgccat
    39721 gattgcaaaa acaaaatcaa aaagaatttt caaacctata acctcctccc cttgccagat
    39781 taggccgggg tagcagacag caagactatt atagcataat tgaaatatcc aaatgattgg
    39841 atttttgcta taattttagc gaggtgattg tgatgaacaa atatgttggt ttgcttgata
    39901 aaatccgagt aataaaaacg caaccgttat tggttcgctt tactttacaa acgattcatg
    39961 aatcgattaa ttgtgtagtt gcagatattg aaattatcga taaattgcta attatggatg
    40021 atggaaaata caatattgcc gtcacaggtc acttcaataa acgaaatcag ctagttattg
    40081 ctagcatgta tgttagaaat cccgatcatt ttacgcgatc gatgggcata taaataaaag
    40141 aagagttgta gtcctaaatg aggattgcag ctctttttgt tttacataga aacaggaaaa
    40201 gaccttttta ggaagacaca agtgccttct caattgaaaa acctttctct cactccaacc
    40261 gccaccgccg ttcgagaaga aaggaagtga acgctgcacg ttcactaaga tatggtgtct
    40321 tgcaaagcaa gacaaaatag acaaaacttc ctctcaccaa aaggttcgtt cctgttcgtt
    40381 atctctttat tgatttgcca gaccggcaac aagttgcctt ttatcaccaa aaagcgacaa
    40441 ggacaagggt aaatggcacg ctgcgcgccc ttgtccttgt cgcttttcgt tgatttattc
    40501 tggccaacaa atcaataaag aaagaactaa cgaaaggaag taaaaccttt cctaagagga
    40561 ggcagaatca aacatgcgaa agaaatttga ttattggggt gttcccttca gcgaattatt
    40621 cccaaactat catgcaccac atactgttga atgcgattgt ggagaaagag cgaaatgtat
    40681 caaaagctat cgtttgtatc aatgtccaac atgcgggaaa aaatatacct tgtcttatgg
    40741 cgattacatt ttgattgatg agaaaggaaa gaaacgctga tgaaaacaca acacaaatta
    40801 ttgactgtaa aagaagtcgt tcaaattttg gtggacaaag aagtcatttc tgaaaaagta
    40861 gcagaagctt tacttattcc gcggtactgg acgatagaaa cccctttagt agaggatgca
    40921 ccgactatgc tatttgaaga cctagtgaag attgcaggat atgaactgca ttatcaagaa
    40981 aacatggaaa cttatgaaat cgaatattgg ttaaccaaat tagagaacta aaaaggagaa
    41041 aaaatatggc tcaaaattac tatgacgaat ttgtcaaatt acctttggat aaaatggcac
    41101 aaaaaatgga ggatatgacg ttcctatacc atgaaacacg tgtacccaaa aaacattaca
    41161 aggaaaaatt gtcagtcgca gtggaagaaa tgattgaatc aggtgtcgaa atgaatctca
    41221 ttgcgacgta ctatcgaaca ctggaagaac tgaaaaaaca aaatggaaaa tggtttttcc
    41281 aagccttact atgcttggaa gtaggtgtca aaccaagcac tatcaaacct tccgaatatc
    41341 aagccttaga actgacttat gcaaaattcg cacagattaa gaaagctaaa gcaatggcta
    41401 gcgactggct agatactttt gaaagtatcg aaaaaaatgg tgcattttat acgattgaat
    41461 cggaggagaa agataatgaa taaggtggtt tatcagctaa caacagaaga tgtttcgcaa
    41521 tttttaacaa gcaaagaaca cttgaatgaa gtaatcagac ggattcaaaa agactttagt
    41581 tgtatggaag aaatgaagta ctttattcaa agagtggaag aacagttgta tatggaaagc
    41641 gaaacaagtc aaacgaaggt cacagtcagc cataatcatt tagacgtgta taaacgtcgt
    41701 cctgaaacgg taaaaaaagg aacagagctt ctacgactag aaacagaaaa atacatcatg
    41761 aatgtgtacc tagaaaggag tgagctggtc gaaaactttg tcagctacac gatttgtagg
    41821 aaacactatt actcagaatc agaatattat tcaattttcg agagcatgga attaatggaa
    41881 gaagaaatgg aagagctaaa cagcaaaaat gcgacggtag acgattatta ccttgtattt
    41941 actaggtttt tgacgtgtgg gacggaatct atcgtgttaa tgggaatgga ttatgaactc
    42001 gctgtacccg aagcattttt gagggaatat gtagaagaag tagataaccc aggcgacgac
    42061 tggtacacga ctttaactga ttttttagac acctacactt acgacgatac agaagaaatc
    42121 caaaatgaac ttgaccgtaa acgaatgcca tatcaacgat ttgaaaggaa aaaaggtaaa
    42181 taaccattta taccgaaaag tgaggatgtg tgaaataaga acaaaaaacc ttgaaattaa
    42241 gggaatggtt cttatttcgc accaatcgct ccaaccgccc ccgccgctta ggtggtgcga
    42301 aataagcggg caaagcccga taacctaaga gaaagaagga gatccatatg aataaaatca
    42361 ttcctctatt gttcgtactg tttgttggcg agatacttat gcgtcatgct gcagctgtac
    42421 atttaaactt cgtagtattc ttaggaatac tggtattttg tttgatcttt tggcgatttg
    42481 tattagccct tgtgagtcta ctattcaaga tgattgcagc catatttttc atagggttat
    42541 ttcgatgaaa ggagagagta ctaatgccaa ttgcaatagg aaataaacgg ttacccgtga
    42601 cattagatga aaagagacaa aaagaattgc agcaactaaa gcagaagtac ggaaaaagtg
    42661 aatctaagat tatgtgtatt gcgttagatc tattgatcgc gcaagaaaaa gcaggatttg
    42721 atataccagc actaaaaaag tgacgtcact tttcatttga agaaacaaaa gtgatagcac
    42781 ttaattgttc ctactattta gaatattttc tatgtccttt ttattccatt ttgtatcaat
    42841 aactatgaaa tctttaaagt atccagaaga gattattttt agatcaattt ttttcttacc
    42901 tgattctaaa gattttgttt tcttaaacat attatgattt cctttcgact attttcatat
    42961 aaatgttaac atagtttatg gattcatgga atgttaatct aatttaatag aaaaggataa
    43021 ttaagtatat acgttttttc ttatgtatgt tatactatgt atatacagaa agaggtgtct
    43081 actatggaaa tgttacctat aaaaaaatgg ggaaatagta atggccttag attgccaaag
    43141 tatattatgg aatatcttgg aattcataca gaagacaaag tgaagataat acaagaagaa
    43201 gtaaacggtc agaagcggct aattatcgaa gcggctaata tagaaaatga attaacaatt
    43261 gaacagttat ttgaaaatta cactgatgaa agaaatcatg tgacgattca aaatttaggt
    43321 gaagcagtag gtaatgaaaa atggtgagaa aacctagaca aggcgatatt ctattattga
    43381 atacagcacc tcgatctgga cacgaacaga caggtaaaag accttatatt gttttaagtc
    43441 atgatattat tgcggattat agtaacgttg ttacagttgc tccaatttct agtacaacta
    43501 gaaactatcc gctatatgta aatataaact cagaatatga aatgaagaca acaggaaaag
    43561 tattattaga tcaactaacg acaattgatt atgaagccag agagtgtatt tttttggaaa
    43621 aagcgcatga tagtttagtt gaggagttat tgatgaaagt tagaacagtt tttcaaaaag
    43681 taagcaaata aaatagaaac agcagcatga tcaactattc gtgattgacc atgctgtctt
    43741 tttttattct atatacggtt tgtctcgtaa cattgtagtc ttttgcaatc ttagcgatcg
    43801 caacaccttt ttttagatcc tcaacgatgt ttttataaac aagacgacgt tggggatctt
    43861 ttgcattggc actgtatagc ttaggacgtc ctttataaac accgttcgct tttgcaatct
    43921 taatgccttg tgcctgacgt cgtttagatt cggtacgttc ttgttcagcg atcatggcca
    43981 aaatttgaat gatcaaatct ttaatgaatt tatccaggag aggattgcca attgcttcgg
    44041 ccatgatcgg tagacttgtg atgatcagct gcacattttt cttcttcaag taattgactg
    44101 aagcgatgat ctcgtcgtaa tttcttccaa gtcgatcaat agcttccaca ataaaaatat
    44161 cttgatctcg gacgaaatcc agtgcttctt gaaaaacaag tcgatgagta atcgttgcgc
    44221 cagattgttt ctcaatgaaa attctctctg caccgaattt tttcatttct tcaatctgcc
    44281 gttgttcatt ttgatcgatg gatgagacac gtatgtatgc aactctcaaa atttttacct
    44341 actttctgca actatttaaa aaacacccta atcatacacg agaaaaccag atttttcaat
    44401 gtcgcggtcg tgtctagtta gggtatacct tatatagaca aaaactaatg atggaggaaa
    44461 tagtatgtgt cacttttctt tttaaatggc aatatttttt gtaaataaat gtcaatcaag
    44521 aaaatttaaa cgattatgac attaaaaata tattttatga attttctagt atataacttg
    44581 atttatctgt taagatttat taagaaaacg ttttcttatt tttgatttcg aggtgataga
    44641 caatgaaaaa taagtttatt ttatctttag gtattttaac tattggaatt agtgtaactg
    44701 gaactacaat tcctacattt gctgatagta caaatgaacc aactactgta gtagataata
    44761 accaagaatt aactccttta gataggaacg gaatagccat tggtaaccca actaacgact
    44821 taactgttac tgaaaattat ttaatagttt ctccattact tgctcgagca gcttctaaaa
    44881 cgatacgagt gacaggaacg atgatttttg ataaagttgg ttggccttct agacctcctc
    44941 aatcacggaa ttggaaagaa aaaagaaatg gttattggta ctatggaaaa ttgtatttac
    45001 aaagttataa atcagctgga aacaattgga ttgcgactta ttcaggaacc ttaactagaa
    45061 agtagggatt ttttatgcca gaatttgtga acacaccaaa tttttatcaa gatagcgata
    45121 ttctaagtaa aattaattta ccgaaagaag aatcagcttt acaagttcag cgaactttac
    45181 aggagacaca aaaaattcaa aaatcaattt ctaatctaga agattctgaa gaaaaaagaa
    45241 aaaaaagaga ccaaaaaaca tatcaaaaag aaaaataatg attttaaaaa aagagtgtct
    45301 agtcgacatt ctttttttct aattgaaagt gttcataaaa tcacccattt ctggacactt
    45361 tctttttttg ctttattttt caaaaaaaca attcatttca tgttcataaa cccataacaa
    45421 tatctatgtt cagttaaact atatggataa agttatgtta caataaaatt aatgaaggag
    45481 tgattttttt gaaaatagga tacgcccggg tatctactgg tttacaaaat ttggatttac
    45541 aagaagacag cctaaataaa tttggttgtg aaaaaatttt tactgatcat atgagtgggg
    45601 caaaaagtaa tcgaccagga ttagaaatgg ccattgattt tgttcgctct ggcgatacac
    45661 ttgtcgtttg gcgcttagat cgtttaggta gaaatatgga agatttgatt tcaatcgtca
    45721 atcgactgaa tgagcgtggt gtgagttttc atagtattca agaaaatatt acgatggaca
    45781 aatcaagttc tacaggacag ctcatgtttc atttgtttgc ggcatttgca gaatttgagc
    45841 gtaatttgat cctggaacga tccgcagcag gacgagcagc tgctcgtgca agaggacggt
    45901 ttggtggtcg tcctgaaaaa ttgtctgaac aagatttgga attactgaag actttagtgg
    45961 atagcggtac acctattaag actattgcgg aacggtggaa cgtctcaaga accaccattt
    46021 atcgttattt ggcaaaaatg gaggagaaaa atggaaagac aaattctcat tagtttttgt
    46081 catttaatgc tgaaaatttt tgccatttat ctagaaaagt gacaatagta tgagaatttt
    46141 ttgtaagtgt ctactgataa cttattctca aatgttgtaa ggattttatt catttgttaa
    46201 tattaa
//
LOCUS       DAZKLM010000011        45770 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3764
            SAMEA115706913-rid22582563.denovo.011, whole genome shotgun
            sequence.
ACCESSION   DAZKLM010000011 DAZKLM010000000
VERSION     DAZKLM010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706913
            Sequence Read Archive: ERR13196647
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 45770)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45770)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287823.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287823.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,004
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,849
            CDSs (with protein)               :: 2,849
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 41 of 96
            Pseudo Genes (incomplete)         :: 52 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 15 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45770
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706913-rid22582563.denovo.011"
                     /isolate="VRE3764"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..731
                     /locus_tag="ACG842_000562"
     CDS             <1..731
                     /locus_tag="ACG842_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HFQ0247125.1"
                     /translation="SILLLLTLVVGYFSIEFIHGFSSAKQSSTVKKVEPKSIPTTLNV
                     ALIGSDARSKDENGRSDSLLVAQYNQKTQQAKLISIMRDSYVDIPGYGMNKINAAYSY
                     GGVDLLNQTLKENFKFEAPYYASITFQDFIDCVNELFPGGVKIDAEKSLDLDGVYINK
                     GEQVMDGNTLLQYARFREDKEGDFGRIRRQQQVIKAISQQLKDVTSIFKLPKAVGKLL
                     GSIQTNLPESVLLDCGMDFLKDIC"
     gene            789..2828
                     /locus_tag="ACG842_000563"
     CDS             789..2828
                     /locus_tag="ACG842_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HFQ0247126.1"
                     /translation="MKRSDKHGKNRTGAYIAGAVILIAAAGGGYFYYQHYQETQAVEA
                     GEKTVEQFVQALNKGDYNKAAEMTSKKAANKSALSEKEILDKYQNIYGAADVKGLQIS
                     NLKVDKKDDSTYSFSYKAKMNTSLGELKDLSYKGTLDRNDGQTTINWQPNLVFPEMEG
                     NDKVSLTTQEAARGNIIDRNGEPLATTGKLKQLGVVPSKLGDGGEKTANIKAIASSFD
                     LTEDAINQAISQSWVQPDYFVPLKIIDGATPELPAGATIQEVDGRYYPLGEAAAQLIG
                     YVGDITAEDIDKNPELSSNGKIGRSGLEMAFDKDLRGTTGGKLSITDADGVEKKVLIE
                     HEVQNGKDIKLTIDAKAQKTAFDSLGGKAGSTVATTPKTGDLLALASSPSYDPNKMTN
                     GISQEDYKAYEENPEHPFISRFATGYAPGSTFKIITAAIGLDNGTIDPNEVLTINGLK
                     WQKDSSWGSFQTTRVSSDVSQVDLKTALIYSDNIYAAQETLKMGEKKFRTGLDKFIFG
                     EDLDLPISMNPAQISNEDSFNSDILLADTGYGQGELLINPIQQAAMYSVFTNNGTLVY
                     PKLIADKETKDKKNVISETAVQTIVPDLREVVQDVNGTAHSLSALGIPLAAKTGTAEI
                     KEKQDVKGQENSFLFAFNLDNQGYMMVSMLENKEDDDSATKRASELLQYLNQNYQ"
     gene            complement(2937..4088)
                     /locus_tag="ACG842_000564"
     CDS             complement(2937..4088)
                     /locus_tag="ACG842_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HFQ0247127.1"
                     /translation="MEFIGILCLILISTTIGSHISRRLGIPAVIGQLLVGVLLGQAGL
                     NWVKPDILVHDFSEIGVILLMFLAGLESDLSLLKKYFRPGMFVALLGIIFPVVFGWLT
                     GKAFQVSGNEAIFFGIILAATSVSISVEVLKELNVVNTKEGSTILGASVVDDILVVLV
                     LSFSLSFLTGEASSNASLPLVLIEQLIYFVFIFLLVKWIAPFLLSFAEKVYANSSIII
                     VSLVICLGMSYVADLVGLSSVIGAFFAGIAVSQTKVKEEVYHNVEALGYAVFIPVFFV
                     SVGLEVDFSDFSEQLLFISILTLVAILTKLLGGYFGAQIAGFSQHSSLMVGAGMISRG
                     EMALIILQIGQQSQLIEPQYYSPLVIVILLSTLISPLVLKYFTKKVYEV"
     gene            complement(4242..4772)
                     /locus_tag="ACG842_000565"
     CDS             complement(4242..4772)
                     /locus_tag="ACG842_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HdeD family acid-resistance protein"
                     /protein_id="HFQ0247128.1"
                     /translation="MFETIKQNIQKYALLRAAVYIISGIAIVLNPSAVFHFIGYLITA
                     YLVLLGLLNLFEAYRFKRKTGSWSIGLFSGLFFLILALIVYAFAPAIVSILPILLGLA
                     VIINSLFQLFFSMNTKAKSWSVYSSILLLIGGFVLLFNPFKSLMLLFQIFGFILIFMG
                     IQEIINFIRIRKMTIR"
     gene            complement(4871..5221)
                     /locus_tag="ACG842_000566"
     CDS             complement(4871..5221)
                     /locus_tag="ACG842_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 domain-containing protein"
                     /protein_id="HFQ0247129.1"
                     /translation="MAQNRIFTTSFASVYPMYVAKAERKDRIKEEVDEIICWLTGYSQ
                     TELEYQLQKQVTFEQFFAEAPVPNPSRSLITGVVCGVRVEDIEDNLMKEIRYLDKMID
                     ELAKGKKMEKILRS"
     gene            complement(5378..6907)
                     /gene="ahpF"
                     /locus_tag="ACG842_000567"
     CDS             complement(5378..6907)
                     /gene="ahpF"
                     /locus_tag="ACG842_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008785 - alkyl hydroperoxide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000302 - response to reactive oxygen
                     species [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HFQ0247130.1"
                     /translation="MLDKETKQNLEQYLALIESPIVFSVSLDNSENSQKLAEFTKEIA
                     EMSPKISWEKRDSMRTPSFSINPVGKESGIVFAGIPLGHEFSSFLLAMLQISGRAPKI
                     SESLSLKIKKITQTLHFDSYVSLTCHNCPDVVQALNILAVLNPNITHTMIEGGMFQKE
                     IDDKKIMAVPTVFLNGEEFSSGRMTIEQIVEKITGPLIEEELFEKEPYDVLVIGGGPA
                     ASSAAIYAARKGIRTGLVADSFGGQVVETLGIENMIGTSYTEGPKLMQQVESHVRSYP
                     IDIMMNQQAVSLNKEQHFINIGLANGISLKAKTAIIATGAHWRSINVPGEKEFKNKGI
                     AYCPHCDGPLFKGKEIAVIGGGNSGVEAAIDLAGLAKHVYVLEFLPELKADQILQDKL
                     VSLANVTVIKHAATKEISGTNQVESLSYIDRRTMEMHTLAVSGVFILVGLLPNTDWLD
                     GTIEMNPRNEIVTEKNGATNIPGVFAAGDCTDSPYKQIIVSMGSGATAALGAFDYLMR
                     T"
     gene            complement(6917..7480)
                     /gene="ahpC"
                     /locus_tag="ACG842_000568"
     CDS             complement(6917..7480)
                     /gene="ahpC"
                     /locus_tag="ACG842_000568"
                     /EC_number="1.11.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0102039 - NADH-dependent peroxiredoxin
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit C"
                     /protein_id="HFQ0247131.1"
                     /translation="MSLIGKKIEEFNAHAYHQGEFLEVSEKDLMGNWSILCFYPADFT
                     FVCPTELEDLQEQYSTLKSLGVEVFSCSTDTHFTHKAWHDTSDAIGKIEYVMIGDPSH
                     QISRIFDVLDEEQGLAQRGTFIIDPDGIVQAMEINADGIGRDASALIDKIRAAQYVRT
                     HPGEVCPAKWKESGETLKPSFDLVGKI"
     gene            complement(7624..8286)
                     /locus_tag="ACG842_000569"
     CDS             complement(7624..8286)
                     /locus_tag="ACG842_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFQ0247132.1"
                     /translation="MKSSLQSQAYQAIRKQIIYADLEPGRKISEKALEEHLAIGRTPI
                     REALIQLRQQELVYTIPQSGTYVSKINLVSAENARFVREQLEEQIMIECCSKLDTQTR
                     IVLETILEEQEKAVKINDERAFFRADNLFHEACFEIAGRKEVWNWLDDHNTHLERFRW
                     LRVTIQGLPWETIMEQHYDLLKALVDKKPEEARFQTGLHLHMMLKEQETVTQRYPEYF
                     QQ"
     gene            8470..10116
                     /locus_tag="ACG842_000570"
     CDS             8470..10116
                     /locus_tag="ACG842_000570"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HFQ0247133.1"
                     /translation="MIDLNNWRNFIPELSQKEVEIPHFDRASLRENGQKNPVWIHFGG
                     GNLYRGFHAEIAQRLADLGELSTGVIVCETFDEEIIERAYQDFDDLILEVVMPSSGEF
                     VKRILAATADSLYCHPDCSEHFDKMIRHFENPSLQLVTFAITEKGYRLKDTNNKYIGP
                     IAVDIKNGPKKPSHTMSILAALLYKRFLSGAWPIALVSTDNFSQNGSQLRTSLLTIAK
                     EWQKEGYVQNTFIQYLSNEEIVAFPWTMIDRITPNPSLSVSKMLAKEGFSNMSIFQTA
                     KGNNIAPFVNTEDIHYLIMEDHFPNGRPKFEKAGVFLTDRATVDKADTMKVTTCLNPL
                     HTSLAIMGCLLGYRSIAEEMKDPDLSLLVSEIGYNEGLPVVESPGIIDPKIFLEEVIT
                     VRFSNPNIPDMPQRIATDTSQKVAIRYGETIKKYMEIEVKDPQTLVFIPLTLAAWLRY
                     LIGIDDQGKPFIPSPDPLLTDFQHKLSDIQLGTQDTSHIHEKMAPILSNKEIFGVDLY
                     QAELGRKIEEMFIKMVAGPNAVRKTLQDYLQEHGGNLSWK"
     gene            10107..11189
                     /gene="uxuA"
                     /locus_tag="ACG842_000571"
     CDS             10107..11189
                     /gene="uxuA"
                     /locus_tag="ACG842_000571"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HFQ0247134.1"
                     /translation="MEMTFRWYGSQEEKITLDEIRQIPAVKGIVGTLMDIPVGEVWPR
                     ERIKELKEEIESRGLTLKVIESVNVHDAIKIGLPSREQYIENYKQTIRNLAEYGIEVI
                     CYNFMPVFDWVKSDLDYRLEDGSSTLAFISDDIPDDPREIVARIEHSSNEFELPGWEP
                     ERLAHIKSLLEAYASVDEEKLRENFAYFLQSIIPTCEEVGVKMAVHPDDPPYSIFGLP
                     RIVKNREDLDWLCRIVDSPYNGITLCTGSIAEDPENDVYGILAEFCQRDRIPFAHVRN
                     IKFLANRDFYEAPHKSEYGSLDMYRILKTMYDNGFNEYIRPDHGRMIWNETGRPGYGL
                     YDRALGAVYLNGLWEAIEKGNKSKDN"
     gene            complement(11297..12850)
                     /locus_tag="ACG842_000572"
     CDS             complement(11297..12850)
                     /locus_tag="ACG842_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0247135.1"
                     /translation="MQTQEAIKTFLLKKKSNTKLDIFLFLSEHRFFITTQYLADHFHM
                     SESNFLLYIKELEQDFERLNLTELHIDKQKPFLKLNFEGIDPAYCYYRLFGRYCNESV
                     SYQILTSLFSCQTNSIISLSQQTNYSASYLYTKMKKINAFLALYGLSISFSNNGRKSF
                     SGKEVQIQYAALDIYWNIFSSTATEFYDEDPQVVAFMMNTFFKKEVLDRLNSGMIDKL
                     YFLLKICKENFPATSVKDVQKEFTEYEYIDFFIDPSVDILRPNLPICREQRILVNILA
                     RLSITKIESEETSLEQYKLLKKANVPHVLYSREMVESFCTVFDLFIPEKEKILYSLAF
                     ARNKLYNGFLNFNQPNTPLPTFMLYQEHSVLKEVQTAIEQFYMDFREQNQHLLPVILE
                     KENVQWMVEDLVHLYDRYKKQPRIVIGVNYTRDFYISKDLIVKIEQIFSNESIKIQND
                     FMENCNIVISDCPLKQLPSHIKKIYILKGIITPEDWKKVIMRISEYIFELKEEALMDQ
                     SKKENQVTS"
     gene            12998..14167
                     /locus_tag="ACG842_000573"
     CDS             12998..14167
                     /locus_tag="ACG842_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HFQ0247136.1"
                     /translation="MRYSTILISTFAALSLSLTINQTVHTEEIETAKWTETKWANVNR
                     ITFQDEFSSFNSGVAKLRKGQTATIKAKIDYSGDLAPILQSAVTFENVDPALSIGYDE
                     NTLTIQHKQAIFTFTVTLNQDLLKPALFTVKVSDGLPNDHHHLTPYSQRQTIEQDSAI
                     DSGGDLVEEPTDKPENENKPEVPPTENPDGEQKPEIEPGEEPDTETQPEPDNESKPEI
                     TPGEKPDVDPEEKPDVTPEPDTDSGNQTVPETNPDTDNETENPEKLEVDPEEKPDVTP
                     EPDTDARDQGIPEKINKKTIQEDGKKESKKSNLAILKINEEQLNKKSRIFDSAQSAET
                     LKSSKDTTFASPETKNKQLPKSGESQNKVILWSGIILLSIATMLSAKRFKQNRSL"
     gene            complement(14293..15372)
                     /locus_tag="ACG842_000574"
     CDS             complement(14293..15372)
                     /locus_tag="ACG842_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFQ0247137.1"
                     /translation="MNNVFIFCYLEVVDLTVYQRFLANEKIRRLTVLLFVVFVLFLVR
                     SMITTILLTFIFTYLMIHLVQIIQRFVRIPTGVLAIMIYTLIVYLIYLAFTKYIPMLI
                     HQTFDMIQSVIDFYEHQPKDADPVLQYIHTYIERNDFFEQLQNGASIALGYLQDIGKM
                     AVAFAMSFILSFFFMIEKKKTIVFSRLFLKSEFSWFFQDIYYFADKFVNTFGLVLEAQ
                     FVIALLNTLLTTIALAVFGFHQLLSLAIMIFILSLIPVAGVIISCIPLSFIAYSQGGI
                     RDVVYILITILIVHLIESYVLNPKLMSSKTELPIFYTFVVLLVSERFFGVWGLIVGIP
                     IFTFFLDVLKVKKIPNHLPKETDIP"
     gene            complement(15481..15921)
                     /locus_tag="ACG842_000575"
     CDS             complement(15481..15921)
                     /locus_tag="ACG842_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFQ0247138.1"
                     /translation="MEDLYLVNQVCFPLYAASKEIIRHYTSLLKPLNLTYTQYLVMLV
                     LWEKKKTSVKELGNDLCLDSGTLTPLLKKLAEKNYINRQRETGDERIVNVTLTPQGAA
                     LQKQAEGIPQKIKSYLPLEQEELDTLQHLTRKMLDNFHQDQKKG"
     gene            complement(15978..16388)
                     /locus_tag="ACG842_000576"
     CDS             complement(15978..16388)
                     /locus_tag="ACG842_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HFQ0247139.1"
                     /translation="MKKMYSTKMINTGGRSGEVHNPDRTFELNIAAPGKRVENATNPE
                     QLFAAGFSACFNSALELIKQQKNISGASTVSAQVSLYSESETSFVLGVEIEGSIEGLS
                     LEETQELLEAAHKVCPYSKATAGNIEVTLKAVSE"
     gene            complement(16569..17003)
                     /locus_tag="ACG842_000577"
     CDS             complement(16569..17003)
                     /locus_tag="ACG842_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFQ0247140.1"
                     /translation="MHKGTEIDFVLNDSLKALELYQTIFDEIQLIEATDYPRGNNEVI
                     FTLYGTRFHMLDENTSLGLTAPTAEHPNTIWFNIIVSDIEDTHNKALRNGCVEIQEMT
                     EMMDFGVTNSMFRDPFGYLWMLHQVHREVSFEERKQLFEKYM"
     gene            complement(17103..17951)
                     /locus_tag="ACG842_000578"
     CDS             complement(17103..17951)
                     /locus_tag="ACG842_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFQ0247141.1"
                     /translation="MKETRQFIWTYIKIIFALFILGVAINMFLGPHHIAAGGVSGLGI
                     LLEAVLGFDRAIVILVLNIVMLVLALLFLGKKPFFKVLFGSLFFPLIIAIVPETMITS
                     DRLLSVIFGSAIFALGVAILYKNNSSSGGTTIPPLIFKKYFHLNTSIGLFFTDAFVVS
                     LNLFVFGIEEFLYAILSIAITSIVMTYIETGINRKKSVMVMSEHYLEDIRLRLSAEIG
                     RGLTLLEAKGGYNRKSKEVLLIVVTDHEFSRMKPLIEEIDPSAFVIVSSVAEVMGRGF
                     TYHPIE"
     gene            18340..20106
                     /locus_tag="ACG842_000579"
     CDS             18340..20106
                     /locus_tag="ACG842_000579"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 13 protein"
                     /protein_id="HFQ0247142.1"
                     /translation="MDTAAIYHRPESEYAFLYTNTRFRIRLRTRKNDIKKVYVLCGDP
                     YTITTEKWYQKQRPMKKCLSTNVHDYWEIEVTSETRRLQYAFHVVGEDNTDCFYGDQG
                     IFPYRENVITEPNFYFRIPYFHQIDRFTIPEWVKETVWYQIFPERFANGDKANDPEHT
                     LPWGSKDPDREDFFGGDLQGVRDHLDYLTDLGVNGIYFCPIFKATSNHKYDTIDYYEV
                     DPAFGDKKLLKNLIDEAHKRGIRIMFDAVFNHMGVHSPQWQDVLKNGEKSIYKDWFHI
                     RFFPVDSYQMTDLPETAENIPYDTFAFTPFMPKLNTANPEVQKYLLDIATYWIKEFDI
                     DGWRLDVANEVDHHFWKKFREAVTEIKPDIYILGEIWHSSQAWLQGDEFHAVMNYAFT
                     DSIKDYFAKKKITASQMVSGMNHQQMLYRDQVNEGTFNLLDSHDTARILTLCQGNKEL
                     MKSVMAFMFLQKGSPCIYYGTEIGMTGEDDPDCRKCMIWEKEEQDLELFGFIKELVSL
                     RKQLSKIISEGSTQWLIVYDREDKLYFTRELEGQIIYVYFNQSKEPWVVEQENEVILS
                     QNCQLLEDGKAEIRQYGFLIMC"
     gene            20208..21467
                     /locus_tag="ACG842_000580"
     CDS             20208..21467
                     /locus_tag="ACG842_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFQ0247143.1"
                     /translation="MKKRSVKQLGLGLISLGILGGLAACGNGNSSAEGEADEGKTLTV
                     SVDSGYKDYINEIKNTFEKENDVKIKLVEKDMFDQLESLALDGPAGKGPDVMMAAYDR
                     IGALGQQGHLAEVKLGNEAAYDETDKAQVTYDGKIYGEPAVIETLVLYYNKDLVDTAP
                     STFKDLENLSKDSRFAFESEAGKNTGFLAKWTDFYYSYGLIAGYGGYVFGDDGTNPSD
                     IGLNNAGAVEGISYATDWFKNVWPKGMQDIKSAGDFASQQFMSNKTAAIIDGPWQAQT
                     YKENNINYGVAKIPTLNNGQPYQPFGGGKGWVVSNYAKNKDLSQKWLDYVTNQENQEK
                     FFEMVNEIPANQQARETAKAKNDELTTAVIEQYETAQAMPNIPEMGEVWTGAENLMFD
                     AASGSKTPKESADEAVKTISEAIEQKY"
     gene            21614..22915
                     /locus_tag="ACG842_000581"
     CDS             21614..22915
                     /locus_tag="ACG842_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0247144.1"
                     /translation="MQQDKTSNINEKKITLCSLIPGMGQFVNGQAFKGIVFFAIFVLF
                     IGQMLLGGFHALVGLVTLGSIPMEDHSLFILIEGTLQFIITIIFLIFYGLNLLDAYRV
                     AKMRQEGKKINRTMKEVIHNIGDEGFPYLLTLPAYLLMIFTIIFPVLVTLFTAFTNYD
                     FNHIPPASLIDWVGAKNFFSIFFLSSYRNTFFAVFSWTVIWTLCATTLQITLGILTAV
                     VAHQKFIKFKRIFGIIFLLPWAVPAFITIMSFSNIFNDSVGAINTQVIPFINQLPFVD
                     ISAISWKTDPNWTKVAIILVQGWLGFPYIYVMVTGILQSISEDLYEAAKIDGANAWQR
                     FSNITLPAVFLVAAPTFITQYTGNFNNFSMIYLFNNGGPGSVGGGAGSTDILISWIYK
                     LTTGTSPQYSMASAITLIISVIVICVSLLVFKKTNAFNMGD"
     gene            22918..23769
                     /locus_tag="ACG842_000582"
     CDS             22918..23769
                     /locus_tag="ACG842_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HFQ0247145.1"
                     /translation="MKKFHSSARFQKRLSHFFTYFFLLILSVIIIYPLLITASTAFKS
                     GNIAAFSLDLSSDWTLGNFTRLFSETLYGTWYKNTLIISVLTMIVQVTVVTLAGYTYS
                     RYRFIGRKSSLMFFLVIQMVPTMAALTAFYVMAFLLEALDKYWFLTMIYIGGGIPMNT
                     WLMKGYFDTVPIDLDESAKLDGAGHFRIFFQIVLPLVKPMLAVQALWAFMGPFGDFML
                     AKFLLRTPENQTVAVGLQTFISDVTNQRVSLFAAGAILIALPICLLFFFLQKNFVTGL
                     TSGGTKG"
     gene            23782..24597
                     /locus_tag="ACG842_000583"
     CDS             23782..24597
                     /locus_tag="ACG842_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrose utilization protein MalA"
                     /protein_id="HFQ0247146.1"
                     /translation="MQTFPLNYFSALRTPTQVFAGRKLLSWPKFFLIFVFLVSLMVMP
                     VTLFYANQIQAIPLEQFLSVHSLIDEQGTQKFSELELSETGLQASQQTIAVTPEILVG
                     VSLSEKQQSDHGTFIDFEKEQWVIQQKDKSGIRRYTMNYSPSFQPDSVRTPEDFQRFL
                     EREFYASNRPTIILSYSLSLGLVLFVMTSLILFGASFFLWMTRKSQLSSIHTFKESAN
                     LMLNVMGIGSMAAAVVGFIHFDFILMLSVQTTITVLLLLWVFVKTRFKDKRVE"
     gene            24748..25914
                     /locus_tag="ACG842_000584"
     CDS             24748..25914
                     /locus_tag="ACG842_000584"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFQ0247147.1"
                     /translation="MLEKTLYNQLFSRSFSLPVEVNYWDGTTKQYGDTDTPPQIKINI
                     HEEIPMKEITSNASLALGEAYMDHRIEIEGSIQALINDAYQQANSFLRGKDYLKWLPK
                     KEKHTKQKNKEDIHAHYDLGNDFYKLWLDPTLTYSCAYFNTPEDTLEQAQINKVHHIL
                     DKLFIKEGETLLDIGCGWGTLMFTATKEYNVKATGITLSEEQYDLITKRIKEEHLEDK
                     CRVLLMDYRELKGETFDHITSVGMFEHVGSENLEGYFKVVKDLLKPKGTALIHGISRQ
                     QGGATNAWINKYIFPGGYVPGVTELVDNITKNDLQLIDLESLRRDYQLTLEHWTKNFH
                     QVQDQVTKEMGDSFYRMWDLYLQACAASFESSNIDVIQYLLVQPGNNDIPMHRA"
     gene            26144..26791
                     /locus_tag="ACG842_000585"
     CDS             26144..26791
                     /locus_tag="ACG842_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HFQ0247148.1"
                     /translation="MKKTVIYTSLFAALFLAACQSNTNQGTSGNTSSSSEQRSSQVSD
                     SSTKASTSSSSQTKSSSEASTQSDIKVSAARAIELFQDKYPDTAITSLELDSDWGSYF
                     YKIEGVDDQKEYEARIDAQNEKMETENPETLDRDEQNGQKKKEDGLDVDKLITIEEAA
                     KIAVDKVGSGTATDWDLDKDLGTTYWEVKVKDGNQSTSVKINSQTGEVLSTEQDD"
     gene            26875..27819
                     /locus_tag="ACG842_000586"
     CDS             26875..27819
                     /locus_tag="ACG842_000586"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HFQ0247149.1"
                     /translation="MSLETTFANHIFANPLMNASGVHCMTTQELDELAHSEAGAFITK
                     SCTINERKGNPEPRYFDVPLGSINSMGLPNLGFSYYLEYALAYEKVQENQNQPLFFSI
                     AGMSVQENLEMLEKIEKSGFNGITELNLSCPNVPGKPQLAYDFEATYETLKEVFSIFS
                     KPLGIKLPPYFDFAHFDQMADILNQFPLTYVNAINSVGNGLYIDTEQEAVVIKPKEGF
                     GGIGGEYIKPTALANVRAFYTRLKPEIQIIGTGGIRTGQDAFEHLLCGASMLQIGTEL
                     HKEGPEIFSRIIKELTQIMSEKGYTSIDEFKGKLRTIS"
     gene            27954..29405
                     /locus_tag="ACG842_000587"
     CDS             27954..29405
                     /locus_tag="ACG842_000587"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HFQ0247150.1"
                     /translation="MNDATELAKELKEKEFSFQELVTDIQKKIKKQNHELNAFVALEP
                     FESNSHELLIPQSEKGPLAGIPFPLKMLGQEKKGWLATSGSRLFETHRASHTSNYVRQ
                     AEAIGLVPFGQTNAPEFGFKNITDPVIYGPARNPWNLECTPGGSSGGAAAAVSSGIVP
                     LAGASDGGGSIRIPASFCGLIGLKPSRGTMPVGPYAWRGWQGAAIDFGLTVSMRDTEA
                     LFYGMRSIHSGAPYQVSPVEWQTHPRKKRLKIAFCVDSPIHSQISEEAVLAVKKAVDF
                     LGKEGHDLTEIPYPLDGRKLITSYYQMNGAETAAMIRSIETGIQRAVRKEELEPISWT
                     LFQYGQKLSAATYIDSLHVWDEAAIVMENLHQEYDLFLSPTTAFTAPKISEDLQSDAI
                     RAQMEHISDCSEVEGLDVVSSMFEESLKITPYTQLANLTGQPAISLPTHLTKENMPLG
                     IQFMAARGREDLLFQIGYLFEANHQFHLPNPWK"
     gene            29426..30262
                     /locus_tag="ACG842_000588"
     CDS             29426..30262
                     /locus_tag="ACG842_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HFQ0247151.1"
                     /translation="MKKKRLFALITSAFFILSACGTAAANQEDNSWSNVEKKKELVVA
                     TSGTLFPASYYNDQNELVGYDVDLAKEVAKRLNVKISFKEYNVDGQITSVSKRESDFA
                     ANDFGITKEREKKFSLSETFKYSFDSMIVRKKDDSGIHSLEDLKGKKAAGEPNTSYMR
                     LAEKHGAKLVTYDNATNDQYLSDVANGRTDVILNDYYLQKMAVSALPDIPVKILENVY
                     FNENESGFLLKEGNDALKKKIDGVLKEMKEDGTTKKISEKYFQTDVSVKPKETMAESV
                     SE"
     gene            30262..30921
                     /locus_tag="ACG842_000589"
     CDS             30262..30921
                     /locus_tag="ACG842_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFQ0247152.1"
                     /translation="MYFPLIDFSLMWTSLPFVLQGLVYTLGIGFVSFLLGNLVGLLLT
                     VLGLLDWLPLNVFIRFYLSFFRGIPALVLLFLLYFGLPYQLSALTASVICFTITSSAF
                     IGEIYRGSLAGVSSGQWDAGLASGFSFFGVLKYIVLPQAFRISVPALSNVAMDLVKGT
                     SLAATITVPEIFQKAKIIGGRTFDYFSLYILVALIYWVICIGIEKVQQYLEKRSIHQS
                     I"
     gene            31047..31736
                     /locus_tag="ACG842_000590"
     CDS             31047..31736
                     /locus_tag="ACG842_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0247153.1"
                     /translation="MKTPLTIEIESSLYQYCIEQGGLVVEEVTMPDDQGIVDTLSCLT
                     KFDGSREWRCYELKVTKADFRSTAKLSFVGHYNYFVLPSKLYEEVKTEIPTGIGVLVY
                     RPYTVEEEMPASGTFLIAKKAAKKELAVPEEALTNRFMASLFREVRKAKQMERGVKYF
                     PKDQLYKELKRRSENQDPFSNERYYERFLEEIASQAINELQEEVIALQHDYEYLRQQQ
                     QIRRLPTEPLE"
     gene            31742..32698
                     /locus_tag="ACG842_000591"
     CDS             31742..32698
                     /locus_tag="ACG842_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sce7725 family protein"
                     /protein_id="HFQ0247154.1"
                     /translation="MYYPYLRGKQFDLLALKEALSRGLLSNKIQPVIEPVRDSATLKN
                     VIELFQKKHHPIAVIQNPQVGQFKLFDQHVHSWTVKENSSVVPAQILTPENLSEVLDS
                     PPAFLVFDGQHYPKDTEVWKQLAGVDSKFLIPDTSRFRIWLPENKIVIRDSFQTRKHV
                     ESYADKTDDFFSDDYLFFHADGYIGFSDFTIEGSRYFDKGFPSRALAIHLTYIDAYGN
                     IRVKHFVSDSNDSAKDQALKFLEAGDKMKKWIMRHHHQLLITSGMIELLALYQQQKFP
                     GLGVLKKWSLMHHLELISHLLDHPKDWLKYYSKVQELYEVIQ"
     gene            32742..33254
                     /locus_tag="ACG842_000592"
     CDS             32742..33254
                     /locus_tag="ACG842_000592"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0247155.1"
                     /translation="MYIRQTRKEEVEEVVEIINAAADFIASQGSPQWQNGQKPTKETI
                     LNDIERKESYVLIVEDQIAGTAALVSGVDPVYTKIDGSWKESTGPYLSIHRVALAEST
                     RGKKLAKPFLEHLLTIAASKNIQDIRIDTYPANEPMEKTIYSAGFTYQGMIEFPFEHG
                     ERKAYQIIIN"
     gene            complement(33510..34058)
                     /locus_tag="ACG842_000593"
     CDS             complement(33510..34058)
                     /locus_tag="ACG842_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HFQ0247156.1"
                     /translation="MNYCYVFIGPSGSGKTSLAEAIFQPEQKIITYTNRSPRPGEKDQ
                     EDYYFVSDNTFNQMIKGNEFAEWDQYAQHKYGSSKAEIKKKLTVGNCYAVLTANGFWH
                     LYEYFGTVVRPIFITISKETLQSRLKKRGDSSEQISARLAVYVEDLAQLKRLKAIPSL
                     LVVSNDGAFQEAADKIKKGLLI"
     gene            complement(34147..35328)
                     /locus_tag="ACG842_000594"
     CDS             complement(34147..35328)
                     /locus_tag="ACG842_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside transporter C-terminal
                     domain-containing protein"
                     /protein_id="HFQ0247157.1"
                     /translation="MSYLLGIIGLFVTLGLAFLISSNKKAIKLKPLIVMFVLQLVLGF
                     VLLRTTFGTAVVSVLAKMFDHLLAFAGQGVDFVFAGVANKGSAPFFLSVLMPIVFISA
                     IIGILRYIKILPLFMKAVGLGLSKINGMGKLESYNGVASAILGQSEVFISIKKELPFL
                     SEKRLFTMSVSAMSTVSMSIVGSYMALIDSKYVITALVLNLFGGYILASIVNPYVLEE
                     KEDELIIEENKEQTFFQMLGEYILDGFHVAITVAAMLIGFVALIAMINAIFHGIFGIT
                     FQEILGYIFAPLAFISGIPWKEAVDAGSIMATKLVTNEFVAMTELANGNFHFTERTTA
                     IISVFLVSFANFSSIGIISGAMKGLNEEKGNLVAKHGLKILLTATLVSFLSAIVTGLL
                     V"
     gene            complement(35564..37072)
                     /locus_tag="ACG842_000595"
     CDS             complement(35564..37072)
                     /locus_tag="ACG842_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HFQ0247158.1"
                     /translation="MKKIGFDSEKYIEEQSAYILERVNHYDKLYLEFGGKLVDDKHAK
                     RVLPGFEEDAKIKLLQKLKDQAEILICVYAGDIERNKIRGDYGITYDMDILRLIDELR
                     GYGLLINSVVITRYSGQPATKVFINKLERRNIKVYKHSEIEDYPVNVEKIVSEDGFGK
                     NEYIETTKPIVVVTAPGPGSGKLATCLNQLYHESQKGHAAGYSKFETFPVWNVPLKHP
                     LNIAYEAATVDLKDVNMIDSFHFDAYNKVAVNYNRDVETFPVIKRIIEKITGKESVYQ
                     SPTDMGVNRVGFGIVDDTVVQEASKQEIIRRYFQTECDFKKGLIDEEAVNRIKLIMEE
                     VGLRPEDRNVVVPAHDYADKIQAQSPTKDPSAVIAIELPDEVIITGRTSSLMDASAAV
                     ILNAVKHLAHIADDIPLLSPLVLETIQGLKSKTLHSSLDALSINEVLIALSISAVTNP
                     IAQVAYDKLAELEGAQAHSTVMVNKNDEQTLKKLGIDITCAPVYPSENLYYQ"
     gene            37231..37422
                     /locus_tag="ACG842_000596"
     CDS             37231..37422
                     /locus_tag="ACG842_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0247159.1"
                     /translation="MKKRQKKKNAYKHYIRSIFTGYERMLEDPELEQLTFTYLNEETQ
                     LTRDEHQRIHFTTRDLPSK"
     gene            37625..38542
                     /locus_tag="ACG842_000597"
     CDS             37625..38542
                     /locus_tag="ACG842_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coiled-coil domain-containing protein"
                     /protein_id="HFQ0247160.1"
                     /translation="MKEPDTNANYEPDELEETNADQDTGQESSSFYDIKEKASMNLSQ
                     LLEDLQAFEKAVREEDIPEIYRLYNGRLNEELKRSSNENHEIDQLLMRKIHDRFLQEF
                     PFMEQVENISPTMSYYKIGTYYHDRPTVGIDASLPEIFVLPKIDEEWEKYSQPESTDY
                     DKKINELDAKVITAQTEIERLDEQIKEINRQMTDLNDNKGFLNRKKIEEEIQELEKKK
                     QVLSNEKLGWLPYIETPETIQQQKEALKQEARADQLRAAIVEKEQRQIRRYFGSKEGF
                     GQAIHEFLMNYLGNENPNNQSNEGGSEYE"
     gene            38535..39608
                     /locus_tag="ACG842_000598"
     CDS             38535..39608
                     /locus_tag="ACG842_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0247161.1"
                     /translation="MNEHDEAEWTKNEEQTEDHEETNTSLEEQTSNPLDDTEEIPMLE
                     ETKIHEKEKVTPTNTNTAENTHAYEITENPGYLEGKEQDHQDIVSLIETAEAELADLR
                     MRKDLIESDFPQLLESYDRFILNEEPISTIGKTSLIEYFTYELLKPLNDIGLERSNSA
                     YDTWKTRHFSINYTLNGQNELVFSFVLPTIDQRYQVESMSLLNVSPETMEINVQDDRV
                     LSLIRHWSVDRVFSAGQITIFNHKLNQLLAHARTLGFTVNQTLLDNTKPLRLNLQSEF
                     ELTNEVLDDIFIVAMKNPSYDMEKINETTYQVRLDKGQSLTITTNEKDQTVLSISSGD
                     YPRSVIDFFINYEFLVPLMVRKS"
     gene            39756..40274
                     /locus_tag="ACG842_000599"
     CDS             39756..40274
                     /locus_tag="ACG842_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0247162.1"
                     /translation="MSTNKIAQLSLLSAACVAGRIAFQFIPNFQPMTAIFLFIVLYLS
                     LKDALIVMSLSIAISSFYFGVGPWVIGQWISYTVVLSLFSIVCLRKTVQRNLWLKGVC
                     FFLAGMVYGIGMTVFDTLLYRLPQPWIYYLQGVSFDLMHSIGNVVFFLVFLPVVKRFY
                     NHSGGKNEKNNL"
     gene            40255..40647
                     /locus_tag="ACG842_000600"
     CDS             40255..40647
                     /locus_tag="ACG842_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4430 domain-containing protein"
                     /protein_id="HFQ0247163.1"
                     /translation="MKKIIYSLLAMSVSILILTSCSTTYTQSASNQKEEVKTSQKATI
                     TLQENGQNFSEKEVVFKKGDDLLSILKRNFEVKEDNGFITSLEGHSQDEKNQLYWMFT
                     VDGKSATTGAKEIKLNDGQSVIFNLSKL"
     gene            40723..41658
                     /locus_tag="ACG842_000601"
     CDS             40723..41658
                     /locus_tag="ACG842_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HFQ0247164.1"
                     /translation="MENFKKSGMFSVIAALGANILVAISKFVGYAISGSAAMLNESIH
                     SIVDCSNQILLLFGDKQANKGQSELHQFGEGRAKYFFSTIVAMMLFFGGGALGVMEAF
                     EKLTHPSHEVGNPIIVIAILLFGLVIEGSSLRVAMKEIKELNTEKLSTMRFLRESRHS
                     EILIIFTEDLCAVIGLVLALAGTILTYATGIAAFDAISGLLIGFLLMGAAIFLAKEFY
                     SLIIGESVTASDLSKIKLAFERPEISRLIDIKTVHLSPTEILVAAKADIVGNKENEAY
                     SVINDIEKNMRQSLPDKKMYIYIETDKYDPNYSRK"
     gene            41791..42570
                     /locus_tag="ACG842_000602"
     CDS             41791..42570
                     /locus_tag="ACG842_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HFQ0247165.1"
                     /translation="MNKKKSAPWLQALHAVMPLCISYIPVGLACGVLLQKVGFDPLLS
                     GLLSILVFSGGAQFLVASMLTTQASFATTLLMVFFLELRYTLLGASLAGFMKKEKRTF
                     LAVFSQSLNDENYAVNYLKFSTDPSWNKRKALYVNWFSMTAWAGSNMIGNFFGSVIRV
                     DADLVHFALTAMFIFMFIMQMKSALLIFTGLFSGVLGVVFMVLFQNTFGLIAATVIAS
                     FAGFMLEKAFKKEKAKKLRDRGKDVNEPLFNFPEQEANHHD"
     gene            42563..42880
                     /locus_tag="ACG842_000603"
     CDS             42563..42880
                     /locus_tag="ACG842_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HFQ0247166.1"
                     /translation="MTKLYLLLLVACLFIVAYIPRLFPMLYFTHRKVPSWFSDWMKYV
                     PVALFAALAFKDVFITHEHLDIAWNIKIAAMILVAGVAYKTRSMALSVLTGLASVFLL
                     SML"
     gene            43124..44734
                     /locus_tag="ACG842_000604"
     CDS             43124..44734
                     /locus_tag="ACG842_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HFQ0247167.1"
                     /translation="MNKKLMQGTFWLTFANLLCKVLGVVYLIPWLSMMGNNQDGMLAT
                     TLYNVGYLPYGLFLMLGTVGFPNAIAKKVAVATKEGDNAACRLIFRSTINIMFVIGIA
                     SAALMYLFAPLLAGISPIANVNNGILAIRSLCPSLIAIPILSAMRGYFQGKNDLRPYG
                     TSLIIEQAVRVIVILAGTYYLRVLTDGTILEAVLISTVASFFGGLAAIIHMYIVGRRK
                     DFFELKDFLISSRYFERENRSASVSIIRETLPFIFVGSVITIVQMIDQVTMKPLLHFF
                     RPEIADQQLEFLFSRASVNPNKLTLILISMVGTVAISSLPILSTMKKKDRIQIEKTVG
                     DSFSIALLILLPSLTGMSLLAGPLYTLFFGYDPESVGYFQMALLASLFFSLFTILSTM
                     LQSLNHHRFAIRLTVEAIILKVIFQVIGLGLVGGYGMSLSNGLAFGIVFVRGYHFMCK
                     EYRIAPLAKISNFFLKTFRSTLIMLAVCFAVFFLLNQRLSMESKSYAMIYCVAVGSIG
                     GLVFLFSQFGKNGFRMLKNVKHHSRSKE"
     gene            44891..45739
                     /locus_tag="ACG842_000605"
     CDS             44891..45739
                     /locus_tag="ACG842_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HFQ0247168.1"
                     /translation="MKLAVVTDSSAYLPERIKNHPDLFVIPIPLIMDGKIYNEGIDIE
                     ADEYYGLLKNSKDFPTTSQPVVGEVLGLYEELKEKGYDTILSIHLSSGISGFVNTLFA
                     MKDDISGVTVIPYDSKITSMPMGHMVEAALDLNDEGQSLDEILAHIDRIRDNTYAYLI
                     VDDLNNLVRGGRLTNGAALIGSLLKIKPILTFSEGKIVLFEKIRSSKKAFARAEEIIG
                     RRNNEIQPPVKLYVIHANNLEVAIAEKEKLQKKYPNAEIEIGHFGPVIGTHLGEKAIG
                     IAISAQ"
BASE COUNT        15181 a         8248 c         8803 g        13538 t
ORIGIN      
        1 tatctatttt gttactgctt acattagtag ttggctattt ttcgattgag tttatccatg
       61 gattttcgtc tgcaaaacag agttcaactg tgaaaaaggt agagccgaaa agtatcccta
      121 ccacactaaa tgtggctttg attggttcag atgctcgttc gaaagacgaa aacggtcgct
      181 cggattcact tctggttgca caatacaacc agaaaacaca acaagcaaaa ctgatctcta
      241 tcatgagaga ttcatatgtc gatattccag gttacggaat gaataagata aatgcagctt
      301 actcttatgg gggagttgat ttattaaatc aaacattaaa ggaaaatttc aaatttgaag
      361 ctccctatta tgcaagtatc acatttcaag attttatcga ttgcgttaat gaactgtttc
      421 ctggtggagt caagatcgat gcagaaaaat ctctagattt agatggcgta tatataaaca
      481 aaggagagca agtgatggat ggtaatactt tactccagta tgctcgattt cgggaagaca
      541 aagaaggaga cttcgggagg atcagaaggc agcagcaagt gatcaaagct atctctcagc
      601 agctgaaaga cgtaacttca atattcaagc tacccaaagc ggttgggaaa ttacttggta
      661 gtatccaaac gaaccttcca gaaagtgtgc ttctggattg tggtatggat ttcctcaaag
      721 acatatgtta gaataaacag gtataaatag tgaaataaag gaatgacaag caagagaagg
      781 aggaaaaaat gaaaagaagt gacaagcacg gcaaaaatcg aacaggcgct tatattgccg
      841 gcgcagtgat cttaatagca gctgcgggtg gcggttattt ttactaccag cactatcaag
      901 aaacccaagc agtagaagct ggagaaaaga cagttgagca atttgtccaa gctttgaaca
      961 agggagatta taacaaagct gcagaaatga catcaaaaaa ggcggcaaat aaaagtgcat
     1021 tatctgaaaa agagatctta gataaatacc agaatatata cggtgctgct gatgtcaaag
     1081 gacttcagat atcgaatcta aaagtagata aaaaagacga ttctacttac agtttttcat
     1141 ataaagcaaa gatgaatacc tcattaggtg aattgaaaga tctttcttat aaaggaacat
     1201 tagacagaaa tgatggtcaa accacgatca actggcaacc caacttggtt tttccagaaa
     1261 tggaaggaaa tgacaaagta agcctgacca cacaagaagc agcaagaggg aatattatag
     1321 atcgaaatgg tgaaccatta gcaacaacag gcaaactaaa acaattagga gtcgttccaa
     1381 gtaaacttgg agatggaggc gaaaaaacag ccaatatcaa agcgattgct tcctccttcg
     1441 acttaacaga agatgctatc aatcaggcga tttcacaaag ctgggtacaa cccgattact
     1501 ttgtcccatt gaaaatcatt gatggagcaa cgccagaact tccagctgga gctaccattc
     1561 aagaagtaga cggcagatat tatcctttgg gtgaagcagc tgctcagctg attggttacg
     1621 tgggagatat cacagcagaa gatattgata agaatccaga attaagcagt aatggaaaaa
     1681 tcggtcgctc aggtttggag atggcttttg ataaggatct tcgtgggaca acaggtggga
     1741 aattaagcat tacagatgca gacggtgtag agaaaaaagt tctgatcgaa catgaagttc
     1801 aaaatggcaa agatatcaaa ttgacaatcg atgcgaaggc acaaaaaaca gcttttgaca
     1861 gtctaggagg aaaagctgga tcaactgttg cgacaacgcc aaaaaccggt gatcttcttg
     1921 cgcttgctag ctctccaagc tatgatccaa acaaaatgac aaacgggatc tcacaagaag
     1981 attacaaagc ttatgaagaa aatcctgaac atccattcat cagccgattt gcgacaggtt
     2041 atgctcctgg atctacgttt aaaataatta cagcagcaat cggtctcgac aacggcacta
     2101 tcgatccaaa tgaagtgttg acgatcaacg ggcttaaatg gcaaaaagac agttcttggg
     2161 gatcttttca aacaacgcgt gtcagtagtg atgtatcaca agtagactta aaaactgctt
     2221 tgatctattc cgataatata tatgcggcac aagaaacgtt gaaaatgggt gagaaaaaat
     2281 ttcgtacagg cttagataaa ttcatttttg gtgaagacct tgatttgcca attagtatga
     2341 atccagcaca aatttctaat gaagatagct ttaactcaga tatcttgcta gctgatactg
     2401 gatatggaca gggcgaactt ctaattaatc ctatccagca agcagcaatg tattctgttt
     2461 ttaccaacaa tggcacactt gtctatccta aattgattgc agataaagag acaaaagata
     2521 agaagaatgt catcagcgaa acagcagtac aaacgatcgt gccagatctg agagaagttg
     2581 tgcaagatgt aaatggtaca gcacattctc tttctgcttt agggattccg ttggcagcga
     2641 aaactggtac agcggaaatc aaagaaaaac aggatgtaaa ggggcaagag aacagtttct
     2701 tgtttgcttt caatcttgat aaccaaggtt atatgatggt cagcatgttg gaaaataaag
     2761 aagatgatga ttcagcaacc aaacgagcat cggaactatt gcaatacctc aaccaaaatt
     2821 atcaataaaa aattgctttt taagtacagt aaaaggactg tggcgttatc gtcacagtcc
     2881 ttttttcaaa taaacggttt tcacgatgtc tctttcacaa cttctttttg atttactcac
     2941 acttcataaa ctttttttgt aaaatatttg agaacaagtg gagaaataag tgtacttaat
     3001 aaaatcacga taactaaagg tgaatagtac tgtggttcga tcaattgact ttgctgtcca
     3061 atttgtaaaa tgatcaaagc catttctcca cgcgaaatca taccagcacc gaccatcaat
     3121 gaactatgtt gagaaaaacc ggcaatctga gcaccaaaat aacctccaag caatttggtc
     3181 aaaatcgcta caagcgtcaa tatagaaata aagagtagtt gttcactaaa gtcagaaaaa
     3241 tcaacttcta atcctacact gacaaaaaat actggtataa atactgcata tcctaaagcc
     3301 tctacattgt ggtatacttc ttctttaacc tttgtctgac tgacagctat cccagcaaaa
     3361 aaagcaccaa tgacagagct aagcccaaca agatccgcta cgtaagacat tcctaaacaa
     3421 ataactagcg atacgatgat aatagaagaa tttgcgtaca ctttttctgc aaatgaaagt
     3481 aggaaaggcg caatccattt gactaataag aaaataaaaa caaaataaat tagctgttca
     3541 atcaaaacaa gcggtaaaga agcattgcta gaagcttccc ccgttagaaa agataggctg
     3601 aaacttagaa ctaaaacgac taaaatgtca tctaccacag aagcacccaa aattgttgat
     3661 ccttcttttg tattcactac gtttagttct tttaatactt ctactgaaat actgacagaa
     3721 gttgccgcaa gaataatccc aaagaaaatc gcttcatttc ccgaaacttg gaatgctttg
     3781 cctgtcaacc agccaaaaac aaccgggaag atgatcccaa gcaaagcaac gaacatacca
     3841 ggtcggaaat attttttcaa aagagataaa tcactttcca gaccagctaa aaacattaat
     3901 aagatcacac caatctccga aaagtcatgg actagaatat caggtttgac ccaattcaat
     3961 ccagcttggc ctagcaaaac accaacaagt aattgtccaa tcactgctgg gatacctaat
     4021 ctccgcgaga tatgtgagcc aatcgttgtt gaaatcaata tcaggcataa aataccaata
     4081 aattccattc tcatcattcc tttgtataaa atatagcccg aacttagcat aacatacatt
     4141 attgaaaaaa taggaaaata tactcacaaa aaaaccaaca atccaaaaga tttttttgga
     4201 ttgttggttt ttatacggtc tattttaata actaaaaatg attaacgtat agtcattttt
     4261 cttattctga tgaaattgat aatctcctgg atgcccatga aaatcaaaat aaagccgaat
     4321 atttggaata gaagcatcaa acttttgaat ggattgaaca ataagacgaa tccgccaatc
     4381 aacaacagaa tactactata aacactccaa cttttagctt ttgtattcat gctgaagaac
     4441 aactggaaca agctgttgat gataacagct aatccaagaa gaatcggcag gatagaaacg
     4501 attgcaggtg caaaagcata gacgatcaga gctaagatca ggaaaaaaag accactgaac
     4561 agaccgatac tccaagaacc tgttttcctt ttaaaacggt aagcctcaaa aagattcaac
     4621 aacccaagca aaacaagata ggcggtgatc agataaccga taaaatgaaa aacagcagaa
     4681 gggtttagca caatcgcaat cccagaaata atataaacag cagctcttaa taaggcgtat
     4741 ttttgaatat tttgtttaat cgtctcaaac atataagaaa acctcctttg acttatttta
     4801 acgtgaacta gtcattttac caaattaaat gttacaaaat gagcgaagga gaagaatatc
     4861 tctggaaaaa tcaacttctg aggatctttt ccattttttt gccttttgct agctcatcga
     4921 tcattttatc taaataacga atttctttca tcaagttatc ctcgatgtct tctactcgta
     4981 caccacaaac aacacctgta atcaaggaac gtgaaggatt ggggacaggg gcttccgcaa
     5041 aaaattgttc gaaagtcact tgtttttgta gttgatattc tagttctgtt tgggaataac
     5101 ccgtcagcca acaaatgatt tcatctactt cctcttttat acgatctttt ctctccgctt
     5161 ttgctacata cataggataa acgcttgcaa aacttgtcgt aaaaatacga ttttgcgcca
     5221 taatcagtca cctttctttt tttcaaatta tttactactt atagtatata agaaaaagag
     5281 aagaaaaagc gattattcaa aaaaacaaaa aggattgtga caaaattctt gtcaccatcc
     5341 ttttttccgc tctctgactt tttacaatct tcatagctca agtgcgcatc agataatcga
     5401 atgctcctaa agctgcagta gctcctgaac ccattgaaac aatgatttgt ttgtatggac
     5461 tatctgtaca atcaccagct gcgaaaacgc cggggatatt cgttgcgcca tttttctcgg
     5521 tcactatttc atttcttgga ttcatttcga tcgttccatc tagccaatcc gtattaggga
     5581 gtaagccgac tagaatgaat actcctgaaa cagctagtgt atgcatctcc atcgtacggc
     5641 gatcgatata ggagagagat tctacctgat tcgtaccgct gatttctttc gtcgctgcat
     5701 gtttgataac agtgacattc gcaagagaga ctagtttatc ctgtaagatt tgatctgctt
     5761 ttaattctgg taagaattct agaacatata catgttttgc taacccagcc aaatcgattg
     5821 ctgcttcaac accagaatta ccaccaccga taacagcaat ctcttttcct ttaaataaag
     5881 ggccgtcaca gtgtgggcaa taagcaattc ctttattttt aaactctttt tcgcctggta
     5941 cattgataga acgccagtgc gcacctgtcg cgatgatggc ggtttttgct ttaagtgata
     6001 taccgttagc taagccaata ttaataaaat gttgctcttt atttaaggaa acggcctgtt
     6061 gattcatcat aatgtcgata gggtaactac gcacatgact ttctacttgc tgcatcaatt
     6121 ttggaccttc tgtgtaagag gtaccaatca tattttcaat ccctagtgtt tcaacgactt
     6181 gtccgccgaa agaatcagcg acaagtcctg tacggattcc tttacgtgct gcatagatag
     6241 ctgcactact agctgctggt ccaccgccaa ttactaatac atcataaggt tctttttcaa
     6301 acaactcttc ttcaatcaaa gggccagtga ttttttcaac aatttgttcg atcgtcatgc
     6361 gtccgctact aaattcttct ccatttaaaa acacagttgg cactgccatg atttttttat
     6421 catcgatttc tttttggaac atcccacctt caatcatcgt atgagtaata tttggattta
     6481 atacagctaa tatattcaat gcctgtacaa cgtcggggca attatgacaa gtcaagctaa
     6541 cataactgtc aaaatgcaat gtttgtgtta tttttttgat tttaagactc aaagattcac
     6601 tgattttcgg tgcccgtcca ctaatttgta acatagccag taaaaaagag gaaaattcat
     6661 gaccgagggg gataccagca aaaacgattc cactttcttt gccaactgga ttgatagaaa
     6721 aactaggtgt gcgcatcgaa tctctttttt cccaagagat tttaggagac atttctgcta
     6781 tttccttcgt aaattcagct agtttttgac tattttcact attatccaaa ctaacagaaa
     6841 aaacaatagg agattcaatc agtgctaaat attgttcgag attttgttta gtttctttgt
     6901 ctaacatatc caccacctaa atctttccaa caagatcaaa acttggtttc aatgtttcgc
     6961 cactttcttt ccattttgcc ggacaaactt cacctgggtg ggtacgtaca tattgagcag
     7021 cgcgaatctt atcaatcagt gcactagcgt cacgaccgat tccatctgca ttgatttcca
     7081 tagcttgaac gattccgtct ggatcaataa taaatgttcc tcgctgagct aatccttgct
     7141 cttcatccaa aacatcaaaa atacgagaaa tctgatgaga cggatcaccg atcatcacgt
     7201 attcgatttt accgatagca tctgatgtat catgccatgc tttgtgagta aagtgggtat
     7261 cggtagagca agaaaagact tcaacaccta aagatttcaa tgtactgtat tgttcttgta
     7321 agtcttctaa ttcagtagga caaacaaaag taaaatcagc tggatagaag caaagaatac
     7381 tccagttccc cataagatct ttttcactga cttctaaaaa ttctccttga tgataagcat
     7441 gtgcattaaa ttcttcgatt tttttcccaa tcaatgacat agtaaaacct ccaataataa
     7501 ttatttattt ataattattg taatttagtt ggagttgcaa agtcaatgga tattttgtta
     7561 tttaataaag attactcaaa aacgaaggtt aattataaaa atagaagagg tcgcaagaaa
     7621 atgctactgc tggaaatact cgggataacg ttgagtaaca gtttcctgtt ctttcagcat
     7681 catgtgtaag tgcaatcccg tttgaaatcg agcttcttct ggttttttat caactaaagc
     7741 cttgagtaaa tcataatgtt gttccatgat cgtttcccat ggaagcccct gaatggtcac
     7801 tcgtaaccag cggaaacgtt ctaaatgggt attatggtca tctaaccagt tccaaacttc
     7861 ttttcttcct gcaatttcga aacaagcttc atgaaataaa ttatctgcac ggaaaaaagc
     7921 gcgttcgtca tttattttga ctgctttttc ttgttcttct aaaatcgttt ctagcactat
     7981 tcgtgtttgt gtgtccaatt tggaacaaca ttcaatcatg atttgctctt ccaactgttc
     8041 tcggacaaag cgagcatttt ctgcagaaac aagattaatt ttggaaacat acgtaccact
     8101 ttgaggtatc gtatacacga gttcttgctg acgtagttga atcaatgctt cgcgaatggg
     8161 agtccggccg atagcaagat gttcctccag tgccttttca gatattttcc ttccaggttc
     8221 taagtcagca tagataattt gtttacgaat cgcttgataa gcttgtgatt gcaagctgct
     8281 tttcatcttt attcctccaa aatcatttct atgaacgtag tataacagaa gaggtctgaa
     8341 aaacaatctg gtatgtcaca ttaactagta aatttgtata aatagtaaaa ttttggttga
     8401 catagcgatt tttattgtat aaaattcact tgaatacaaa aactaatatg ttagtttagg
     8461 aggggaaaaa tgattgattt aaacaactgg aggaacttta tccctgaatt atctcaaaaa
     8521 gaagtagaaa tacctcattt tgaccgtgca tcattaagag aaaatggaca aaaaaatcct
     8581 gtctggatcc atttcggtgg cggaaatctt tatcgaggat ttcatgcaga aatcgctcaa
     8641 cgtttagcgg atctaggcga attgtccact ggtgtcattg tttgtgaaac attcgatgaa
     8701 gaaataattg aaagggctta ccaagatttt gatgatttga ttcttgaagt ggtcatgcca
     8761 agcagtggag agttcgtcaa acgtatatta gctgcgacag ctgacagtct gtattgtcat
     8821 ccagattgtt ctgaacactt tgataagatg attcgacact ttgaaaatcc tagtttacaa
     8881 ttagttactt ttgctattac tgaaaaaggt tatcgattga aagatacaaa taataaatat
     8941 ataggaccga ttgcagtcga tataaaaaat ggtccaaaga agccatcaca tacaatgagt
     9001 attcttgctg cgctacttta taaacgtttt ttatctggag cttggccgat tgcacttgtc
     9061 tctacagata atttttcaca aaacggtagt caattacgta cttctttgct aacgatcgca
     9121 aaagaatggc aaaaggaagg ttatgtacaa aatacattta tccaatactt atcgaatgaa
     9181 gaaattgttg cgtttccatg gacgatgatt gatcgtatca ccccgaatcc ttccttgtca
     9241 gttagcaaaa tgttggcaaa agagggcttt tcaaatatgt cgattttcca aaccgcaaaa
     9301 ggaaataata ttgctccatt tgtcaataca gaggacatcc attatttaat catggaagat
     9361 cattttccca atggacgtcc gaaattcgaa aaagctgggg tattcttgac tgatcgtgca
     9421 acggtagata aagcagatac gatgaaggtg acgacttgcc ttaatccttt gcatacgtct
     9481 ttagctatta tgggttgttt actggggtat aggagcatag ctgaggagat gaaagatcca
     9541 gatttatcgc tgctagtttc tgaaatcggg tacaatgaag gcctcccagt tgtggagtct
     9601 ccaggtatca ttgatccaaa gatctttctg gaagaagtaa ttactgtgcg cttttctaat
     9661 ccaaatattc ctgatatgcc tcagcgaatc gccacggata cctctcaaaa ggtggcgatt
     9721 cgttatggtg agacgattaa aaaatatatg gaaatagaag ttaaagaccc gcagacactt
     9781 gtattcatac cattgactct cgctgcttgg cttcgatacc ttattggcat tgatgatcaa
     9841 ggaaaaccat tcatcccgag tccagaccca ctattgacag actttcagca taagctttcc
     9901 gatattcaac tgggtacgca agatacatca catattcatg agaaaatggc tcctattttg
     9961 tcaaataaag aaatttttgg tgtagatctt taccaagcag agcttggcag aaaaatagaa
    10021 gaaatgttca tcaagatggt agcaggaccg aatgccgtac gaaaaacatt acaggattac
    10081 ttacaagaac atggaggaaa tttatcatgg aaatgacttt tagatggtac ggatcacagg
    10141 aagaaaagat taccttagat gaaatcagac agattcctgc ggtcaaaggg attgtaggca
    10201 cattgatgga tataccagta ggagaggtat ggcccaggga gaggatcaaa gaactaaaag
    10261 aagaaattga gtctcgtggg ttaacactaa aagtaattga aagcgtcaat gtgcatgatg
    10321 ccattaaaat tggattacct tcccgtgagc agtatatcga aaactataag cagacaatcc
    10381 gaaacttggc agaatatgga atagaagtca tctgctataa ttttatgcct gtatttgact
    10441 gggtgaaatc tgaccttgac taccgcttag aagacggctc atccacgtta gcttttatat
    10501 ctgatgatat tccagatgat ccaagagaaa tcgtcgcgcg catcgaacat tccagcaatg
    10561 aatttgaatt gccgggatgg gaacctgagc gcttagctca tatcaaatcc ttgcttgaag
    10621 cctatgcatc agtagacgaa gaaaaacttc gagaaaattt tgcctatttt ctccaatcaa
    10681 tcatcccaac atgtgaagag gtaggggtca aaatggctgt acatccagat gatccgccat
    10741 actcgatttt tggattacct cgaatcgtaa agaatcgaga agatttagat tggttgtgcc
    10801 gcatagtaga cagcccttat aacggaatca ctctttgtac agggagtatt gcagaagatc
    10861 cagaaaatga tgtatatggg attttagcag aattttgtca aagagatcga atcccatttg
    10921 cacatgttcg aaatattaaa tttttagcta atcgagattt ctatgaggca ccgcataaat
    10981 ccgaatacgg ttcattagac atgtaccgca tactaaaaac aatgtatgac aacggattta
    11041 acgaatacat ccgtcctgat catggaagaa tgatctggaa tgagacagga agaccaggat
    11101 acggcttata tgatcgagca ttaggtgctg tttatctaaa tggattatgg gaagcaatcg
    11161 aaaaaggaaa caaaagtaaa gataattagt gaaaaaaact gtaattttat tatgtaaata
    11221 agaaggatgt tcaaattctt gatacataaa tccggaggtt gttaataagc attcggattt
    11281 atgtatctta tttggtctag gaagtaactt gattttcttt tttcgattga tccatgagtg
    11341 cttcttcttt taattcaaag atatattctg agatcctcat aatcactttt ttccagtctt
    11401 ctggtgtgat gattcctttt aaaatataga tttttttgat atgagaaggc aattgtttca
    11461 aagggcagtc gctgataacg atgttgcagt tctccataaa atcattttgt attttgatcg
    11521 attcatttga aaaaatttgt tctatcttaa cgatcagatc tttactgata taaaaatcac
    11581 gcgtatagtt cacaccaatc acaattcgcg gctgtttttt gtagcgatca tacaaatgta
    11641 ctaggtcttc aaccatccat tgcacatttt ccttttctaa aataactggt aacaagtgtt
    11701 gattttgttc tctgaaatcc atataaaatt gttcaatggc ggtttggact tctttcaaca
    11761 cgctatgttc ttgataaagc atgaaagtag gaaggggcgt atttggttga ttgaagttaa
    11821 gaaatccatt ataaagttta tttcgtgcga atgccaatga gtaaagaatc ttttcttttt
    11881 caggtataaa caaatcaaat acagtacaaa aggactccac catttctctt gaataaagaa
    11941 catgaggaac gtttgctttt tttaaaagtt tgtattgctc caagcttgtt tcttccgatt
    12001 cgattttcgt gatagaaagt cgagctaaaa tattcactaa gatgcgttgt tcgcgacaaa
    12061 ttggcagatt aggtcgcaaa atatctacag agggatcaat aaaaaagtca atatattcat
    12121 actctgtgaa ttctttttgt acgtctttca cagaagttgc aggaaaattc tctttacata
    12181 tttttaatag aaaatataat ttatctatca taccgctatt tagtcggtca agtacttctt
    12241 ttttaaaaaa agtattcatc ataaaagcca ctacttgcgg atcttcatca taaaattctg
    12301 tagcagtact tgaaaagatg ttccaataaa tatctaatgc tgcatattga atttgtacct
    12361 cttttccaga aaaagacttt ctgccattat tagaaaagga gatagacaaa ccgtacaatg
    12421 ctaaaaatgc attaatcttt ttcattttcg tatacaaata agaggcacta tagtttgttt
    12481 gctgacttaa actgataatc gaatttgtct gacagctgaa caaagaagtc aagatctgat
    12541 aactgactga ttcgttgcag tatcttccaa atagacgata atagcagtaa gccggatcaa
    12601 ttccttcaaa attcaacttt aaaaaaggtt tttgcttatc aatatgcagt tctgttagat
    12661 tgagacgctc aaagtcttgt tctaattctt tgatgtatag taaaaaattt gattcagaca
    12721 tatggaaatg atctgctaaa tattgtgtag taataaagaa acgatgttca gataggaaaa
    12781 gaaaaatatc taattttgta ttgctttttt tcttcaagag aaacgtttta atcgcttctt
    12841 gagtttgcat gaaagaggct cctttttact taaattgtta aaacttgaaa atattactgt
    12901 ttttttcata aatttagaca aaaaaacagt aaacatatag gtagaatctc tactatctta
    12961 tttcttgcat acaaaaaaat caaggagtga gagagttatg agatattcaa ccatcttaat
    13021 atcaacattt gcagccttaa gcttatcgct tactataaat cagacagtcc acacagagga
    13081 gatagaaaca gccaaatgga cagaaaccaa gtgggccaac gtcaatcgaa ttacctttca
    13141 ggatgaattt tcatccttca attcaggagt cgcaaaatta agaaaaggac aaacagcgac
    13201 catcaaagca aaaattgact attcaggaga cttagcacct atactacaat cagctgttac
    13261 atttgagaat gttgatcccg ctttgtcgat tggctatgac gagaacactt tgactatcca
    13321 acacaagcaa gcgatattta ccttcaccgt tacattaaat caggacttgt taaaaccagc
    13381 gctttttact gtcaaggtat cagatggact accgaacgat catcatcatt taacgccgta
    13441 tagtcagcgg cagacaattg agcaagattc agctatcgac tcaggaggag atcttgttga
    13501 agaaccaaca gataaaccag aaaatgaaaa taagccagaa gtaccgccta cagaaaatcc
    13561 agacggagaa cagaaaccgg aaatagaacc tggagaagaa cccgatacag aaactcaacc
    13621 agagccagac aatgaatcaa aacctgaaat aacccctgga gaaaaaccag atgtagaccc
    13681 agaagaaaaa ccggatgtga ctccagaacc agatacggat tctggaaacc aaacagtacc
    13741 ggaaacaaat ccagacacag ataatgaaac tgaaaatcca gaaaaactag aggtagatcc
    13801 agaagaaaaa ccagatgtga ctccagaacc agatacggac gcaagagacc aaggaatacc
    13861 agaaaaaata aacaaaaaaa caattcaaga ggatggaaaa aaagagagta aaaaatcaaa
    13921 tctagcaatt ctaaaaataa atgaagagca gctgaataaa aaatcacgaa tatttgatag
    13981 cgcccaatca gctgaaacct taaaaagtag caaggataca acctttgcta gtcctgaaac
    14041 gaaaaataaa cagctaccaa aatccggaga gtcacaaaac aaagttatac tgtggagcgg
    14101 aatcattcta ttaagtatag caacaatgct ttctgcaaaa agatttaaac aaaacagatc
    14161 tctctaacat ttgaataatg tctgtgaacg catttttcaa ataaaaagaa atatccagaa
    14221 aacgacccca aaataccaga ttgaaatctg atatttgggg tcgttcatac tttatctttc
    14281 ttcatattta ttttaaggga tatctgtttc ttttggaaga tggttcggga tctttttcac
    14341 tttcaataca tctaaaaaga aggtaaagat cggaatgccg acaatcaagc cccaaacgcc
    14401 aaagaaccgt tcgctaacaa gtaagaccac aaatgtataa aaaatcggca gttctgtttt
    14461 gctggacatc aacttcggat tcagcacata agactcaatt agatggacaa tcaaaatcgt
    14521 aatcaggata taaacaacat ctcggatgcc tccttgggaa taagctatga aagatagagg
    14581 aatacaagaa atgataacac ctgctactgg gatcagactt aaaatgaaaa tcatgatcgc
    14641 taaactcagc agctgatgaa agccaaatac agccaaggcg attgtagtga gcaacgtgtt
    14701 taaaagagcg ataacaaatt gagcttctaa taccaaacca aatgtattga caaacttatc
    14761 ggcaaaatag taaatatctt gaaagaacca agaaaattca ctttttaaaa acaatcttga
    14821 gaatacaatt gtttttttct tttcaatcat aaaaaagaaa cttaagatga atgacatagc
    14881 aaacgccact gccatttttc ctatatcctg taaatagcca agcgcaatcg aagcgccatt
    14941 ttgaagctgt tcaaaaaaat cgtttcgctc aatataagta tggatatatt gtaaaacagg
    15001 atctgcatct tttggctgat gctcgtaaaa atcaataacg gactggatca tatcaaatgt
    15061 ttgatgaata agcatcggga tatactttgt gaatgccaga tagataaggt aaacgatcag
    15121 tgtataaatc atgatagcta aaacacctgt agggattcgg acaaatcgct gaatgatttg
    15181 aacaagatga atcatcaaat aagtaaagat aaatgtgagc aaaatggttg taatcatact
    15241 tctaactaag aaaagaacaa aaacgacaaa taataaaaca gtgagacggc gtattttttc
    15301 attcgccaaa aaacgctgat aaacggtcaa atcgacaacc tccaaataac aaaaaataaa
    15361 cacgttattc attatatgat ggaatatgaa aataagaaag agaaagagca aaaagaaaaa
    15421 ccgctaaatg acgttatctt atgagttggt tttcctcata aaatcgttca tctagcagtt
    15481 tcagcccttt ttctggtctt gatgaaaatt atctagcatt tttcgggtca gatgttgcag
    15541 tgtatccaat tcttcctgtt caagtggaag ataagacttg atcttttgtg gaataccttc
    15601 tgcttgtttt tgcaacgcag caccttgagg agtcagcgta acatttacga ttctttcatc
    15661 acccgtttca cgctgacgat taatataatt tttttctgct aactttttta gtaagggagt
    15721 cagcgtacca gaatccagac acaaatcatt tcctagctct ttcactgaag tctttttctt
    15781 ttcccataaa actaacatca caagatactg ggtataggta agattcagcg gcttgaggag
    15841 ggaagtgtag tgtctaatga tttccttaga ggcggcatat aaaggaaaac atacttgatt
    15901 aactaaatat agatcttcca tttttccacc tccgctgttt tactatagtg tatcatagcg
    15961 gatttacaca aaaaagtcta ttcactaact gctttcagag taacttctat atttccagct
    16021 gtagcttttg aatacggaca cactttatgc gctgcctcta ataattcttg cgtttcttcc
    16081 agagaaagac cttcgataga tccttcaatc tctacaccta agacaaagct cgtttcactt
    16141 tctgaataaa gggaaacttg tgcactgaca gtagaagccc cagagatatt tttttgctgt
    16201 ttgatcaatt ctaatgcact gttaaaacat gcactgaaac cagctgcaaa caattgttca
    16261 gggttggttg cattttcgac gcgtttacca ggagctgcaa tattcaattc gaatgtacga
    16321 tcgggattat gaacttctcc agagcgtccg cctgtgttga tcattttcgt agaatacatt
    16381 tttttcataa aattaccacc tttaattata ttttatacaa ttgaatttta cacaatataa
    16441 ttgtggaaag caaagaaatg gattggcata agataaataa gagaaagcac atgaaacaga
    16501 cgcgctttct cgaatagcaa acatatacaa aaaaatatca gagacaaaaa ctaatctcat
    16561 cataaacatt acatatattt ctcaaaaagt tgttttcttt cttcaaaact tacttcacga
    16621 tggacctgat gaagcatcca taaataacca aaaggatctc tgaacattga gttagtcaca
    16681 ccaaagtcca tcatttctgt catttcttga atctctacac agccatttcg taatgcttta
    16741 ttatgtgtgt cctcgatatc agagacgatg atattgaacc agatcgtatt tggatgttca
    16801 gcagtaggag cagtcaaacc aagagacgtg ttttcatcca gcatatggaa tctcgtgcca
    16861 tacaaggtaa aaatgacttc attattgcct ctagggtaat ctgttgcctc aatcagttgt
    16921 atttcatcaa agattgtttg atacagttct agtgctttca aactatcatt taatacaaaa
    16981 tctatttccg ttcctttatg cataaagatc tcctctcctt ttcagtcgaa cagttactag
    17041 attcttttat tataccagtt aaagaaaacg cattcaaaaa aatagcggat ttacttacca
    17101 actcattcga ttggatgata agtaaatccg cgtcccatta cttctgctac gctgctgaca
    17161 atgacaaagg cacttggatc gatttcttct atcaatggtt tcattcggga aaattcatga
    17221 tcagtcacaa cgatcaataa aacctctttt gactttcgat tatacccgcc ttttgcctct
    17281 agcagggtaa ggccacgtcc aatctcagca cttaagcgaa ggcggatgtc ttccaaataa
    17341 tgttcactca tgaccataac agatttcttt cgattgatac ctgtttcgat ataagtcatg
    17401 acaattgaag tgattgcaat agataaaata gcataaagaa actcttctat tccaaaaaca
    17461 aacagattaa gagaaacaac gaatgcgtct gtaaaaaata agccaatcga agtattcaag
    17521 tggaaatatt ttttaaaaat gaggggaggg atcgtcgtac cgccgctaga agagttgttt
    17581 ttatataaaa tcgcaactcc taaagcaaaa atcgcacttc caaaaatgac agacaacaac
    17641 cgatctgaag taatcattgt ttctggaact attgcaatga ttaaaggaaa aaacaagctt
    17701 ccgaaaagta ctttaaaaaa gggtttttta cctaaaaata gaagagctag taccaacata
    17761 acgatattca acactaaaat aactatagct cggtcaaatc ctaaaacagc ttccagtaat
    17821 attcctagac cgctgacacc tccagcagca atatgatgag gccctaaaaa catattgata
    17881 gcgactccta atatgaagag ggcaaaaata atcttaatgt atgtccatat aaattgtctc
    17941 gtttctttca tttatccact tccttaattc attaataata ctcttatctt attataactt
    18001 acttttcacc ttaagttaac tttaattaat aaaaaaatta ggctagtcta aattattatg
    18061 tttcattctt ctagaatata cttctacgtt aaaaactgtt aaaaaacttt tactttagaa
    18121 aattggagaa attgatcgct taagataaat tcaggacact aaatagtaca ggaagtagat
    18181 taaaactttt aaaagtttta agtctatcta tttggcaagt ttaaaaaata aaaattcggt
    18241 atcaaaaaac tgttaccgat aacatttttg aaagcgttgt cttttgtttc tctatcactt
    18301 atagtgatga cgtcatatta aaacacaagg aggaaatcaa tggatacagc tgcaatttat
    18361 catcgaccgg aaagtgaata tgcatttcta tatacgaata caagatttcg tattcggctt
    18421 cgtactagga aaaatgatat caaaaaagta tatgtgcttt gtggagatcc atataccata
    18481 acaactgaaa aatggtatca aaaacaacga ccaatgaaaa aatgtctaag taccaatgtt
    18541 catgattatt gggaaattga agtgacgagc gaaacaaggc gcttacagta tgcgttccat
    18601 gttgttgggg aagataacac ggactgtttt tacggtgatc aaggaatttt tccttatcga
    18661 gaaaatgtga ttactgaacc aaatttttat tttcgaatac cttattttca tcaaatcgat
    18721 cgattcacta ttccagaatg ggtaaaagaa acagtatggt atcaaatttt tcccgaaaga
    18781 tttgcaaacg gggacaaagc aaatgacccc gaacacactt taccttgggg aagtaaagat
    18841 ccagaccgag aagacttctt cgggggagat ttgcaaggtg tgagggatca tttggattac
    18901 ttgacggacc ttggcgtcaa tggcatctat ttttgtccca tatttaaagc cacatccaat
    18961 cataaatacg atacgattga ttattatgag gtggatcccg cttttggcga taaaaaactt
    19021 ttaaaaaact tgattgatga agcacataaa agagggatac gtatcatgtt cgatgctgta
    19081 ttcaatcata tgggtgtcca ttcaccacag tggcaagacg ttctcaaaaa cggagaaaaa
    19141 tcgatctata aagactggtt ccacatccgt tttttcccag tagattctta tcagatgact
    19201 gacttaccag aaactgctga aaatatccca tatgatactt ttgcttttac tccattcatg
    19261 ccgaaactaa atacagcaaa tccagaagtg caaaaatatt tgctggatat cgcaacttat
    19321 tggataaaag aattcgatat tgatggatgg cgtcttgatg ttgctaatga agtcgatcat
    19381 catttttgga aaaaattcag agaagcagtt acagaaatca aaccagacat ctatatcctt
    19441 ggagagattt ggcattcctc tcaagcttgg ctgcaagggg atgaattcca tgcagtgatg
    19501 aattatgcat tcacagattc gattaaagat tattttgcaa aaaagaaaat cacagcttca
    19561 caaatggtca gtggaatgaa tcatcagcaa atgctttaca gagatcaggt gaacgaagga
    19621 acattcaact tattagattc tcatgatact gccaggatct tgaccctttg ccaaggtaac
    19681 aaagaattga tgaagtctgt gatggccttt atgtttcttc aaaaagggtc tccttgtatc
    19741 tattacggga cagaaatagg gatgactggt gaagatgacc cggattgccg caaatgtatg
    19801 atatgggaga aggaggaaca agatctagag ctattcggtt ttataaaaga acttgtttct
    19861 ctaaggaaac agttgtctaa gattatttct gaaggttcta ctcagtggct aatagtatat
    19921 gatcgtgaag ataagctcta tttcacacgg gaactagagg gacagatcat ctatgtttac
    19981 tttaatcagt caaaagaacc ttgggtagta gaacaggaaa atgaggtgat tcttagtcaa
    20041 aattgtcagt tgttagaaga cgggaaagca gagatacgac aatacggatt tttgatcatg
    20101 tgttgaaaac tgttaccggt aacaacaaga aagcgtttgc aaatgaagat ttgtgcccat
    20161 tataataagg gtgtaacagc acgttgaata cacaggagga aatgaagatg aaaaaaagaa
    20221 gtgtgaaaca gttgggttta gggttaatct ctttaggaat cttaggcgga ttagcggctt
    20281 gtggcaatgg taactcttca gcagaagggg aagccgatga aggaaaaacg ttaacagttt
    20341 ccgttgattc tggatataaa gattatataa acgaaataaa aaacactttc gaaaaagaaa
    20401 acgatgtgaa aatcaagcta gtagaaaagg acatgttcga tcagttggaa tcattagcgt
    20461 tagatgggcc tgctggaaag ggacctgacg ttatgatggc agcatatgac cggatcggtg
    20521 cgttaggaca gcaaggacat ctggcggaag taaaactggg aaatgaagca gcttacgatg
    20581 aaacagacaa agcccaagtg acttatgatg gaaaaatcta cggagaacct gcagtaatcg
    20641 agacccttgt tttgtactat aacaaagatt tagtcgatac agcaccatca acgtttaaag
    20701 atttggagaa tctatccaaa gacagtcgtt ttgcttttga gtcagaagca ggaaaaaata
    20761 caggattttt agctaaatgg actgactttt attattccta tggtttaatt gcaggttacg
    20821 gtggctatgt atttggtgat gatggcacaa atccttcaga tatcggtttg aataatgcag
    20881 gtgctgtaga aggaatctct tatgcaacag attggttcaa aaatgtttgg ccaaagggaa
    20941 tgcaagacat aaaaagtgca ggagacttcg ccagccagca attcatgtcc aataaaactg
    21001 cagcaatcat tgacggtcca tggcaagcgc aaacatacaa agaaaacaat atcaattatg
    21061 gtgtagccaa aattcctact ctaaataatg gacagcctta tcaaccgttt ggcggaggaa
    21121 aaggatgggt agtcagcaat tatgctaaaa acaaagacct ctctcaaaaa tggcttgatt
    21181 atgtaacgaa tcaggaaaat caagaaaaat tctttgaaat ggtaaatgaa atccctgcaa
    21241 accaacaagc tagggaaaca gcaaaggcta aaaacgatga attaacaaca gctgtgattg
    21301 aacaatatga aacagctcaa gcgatgccaa acatcccaga aatgggcgaa gtttggacag
    21361 gtgcagaaaa cttgatgttc gatgcagcat caggttcaaa aacaccaaaa gaatccgcag
    21421 atgaagcagt aaaaacaatc agtgaagcca tagaacaaaa atactgatta aaatgataac
    21481 cgtccatttt tataaaaaat ggaacaggtg cttcagataa aagtggcaag agcgagatgg
    21541 aaaattatct tgctcttgtt gccattatct taaaagcagt ttgcggttta gcaagtttag
    21601 agggggcatt cagatgcagc aagataaaac aagcaatatc aacgaaaaaa aaatcacgct
    21661 ttgttcactt attccaggga tggggcagtt tgtaaatggc caagctttta aaggtatcgt
    21721 ttttttcgcc atattcgtcc tattcattgg tcagatgctt ctgggcggat ttcatgcatt
    21781 ggtaggattg gttactttgg gaagtatacc aatggaagat cattcattat ttattttgat
    21841 tgaaggcact ttgcaattca tcattacaat cattttcttg attttttacg gtttgaatct
    21901 tttagacgct tatcgagtag cgaagatgcg tcaagagggc aaaaaaatca atcggacgat
    21961 gaaggaagtc atacataata taggtgatga aggttttcct tatctattga cacttcccgc
    22021 ttatcttctc atgattttta cgatcatttt cccagtccta gttacattgt tcactgcatt
    22081 tactaattat gacttcaatc atattccacc agccagttta attgattggg taggggctaa
    22141 aaactttttt agtatcttct ttttaagctc atatcgtaat actttttttg cagtcttttc
    22201 gtggaccgtg atttggactt tatgtgcaac aactttgcaa atcacccttg gtattctcac
    22261 agcggttgtt gctcaccaga aatttattaa attcaaacgt attttcggta tcattttctt
    22321 attaccatgg gcggtcccag cttttattac tatcatgagt ttttcgaata tattcaacga
    22381 tagcgtaggt gccatcaata ctcaagtgat tcctttcata aaccagcttc cttttgtaga
    22441 tatcagtgca atctcttgga aaacggaccc gaactggacg aaagtagcaa ttatcttagt
    22501 gcagggctgg ttaggatttc cctatatcta tgtgatggtt acaggaatct tacaatcgat
    22561 ttcagaagac ttatatgaag cagcaaaaat cgacggagca aatgcatggc agcgcttttc
    22621 aaacatcacg cttcctgctg tcttcctagt ggcagcgcca acatttatta cacagtatac
    22681 ggggaatttc aataatttct ccatgatcta tctgtttaac aatggtggtc ccggaagcgt
    22741 aggcggagga gctggttcta ctgatattct tatttcctgg atctataaac ttacgactgg
    22801 aacttctccg cagtattcta tggcttcagc aatcacgttg attatctccg tcatcgttat
    22861 ctgtgtgtca ttgctggtat ttaaaaagac aaatgcgttc aatatggggg attaacaatg
    22921 aaaaaatttc attcgagtgc tcgattccaa aaacgacttt ctcacttttt tacttacttt
    22981 tttcttttaa tactgtcagt cattatcatc tatccgcttt tgatcacagc aagtactgcc
    23041 ttcaagtcag gaaatattgc agcttttagt ttagatcttt caagtgactg gactttagga
    23101 aactttactc ggctattcag tgaaacactt tacgggactt ggtataaaaa tacattgatc
    23161 atttctgtcc taacaatgat cgtacaagtg actgtagtaa ctttggcggg ctatacttat
    23221 agtcgctatc gttttatcgg ccgcaaatca agtttgatgt tctttttagt gattcagatg
    23281 gtaccgacta tggcagcatt gactgccttt tatgtcatgg ctttcttatt agaagcgctc
    23341 gataagtact ggttcttgac aatgatctat ataggcggtg gaattccaat gaatacttgg
    23401 ctgatgaaag gatactttga cactgtacca atagatttag atgaatctgc taaacttgat
    23461 ggggcagggc atttcaggat ttttttccag attgtattac cgcttgtcaa accgatgctc
    23521 gccgttcaag cattatgggc ttttatggga ccttttgggg atttcatgtt agctaagttc
    23581 ctgcttcgaa cacctgaaaa tcagactgtt gctgtcggct tacaaacatt tatttctgat
    23641 gtgacaaacc aaagagtctc tctttttgct gctggggcaa ttttgattgc actaccgatt
    23701 tgtctattat tcttcttttt acaaaagaac tttgttaccg gcctgacatc tggcggaaca
    23761 aaaggatagg agcagagtat catgcaaaca tttccgttaa actatttttc cgcattacgt
    23821 acgccaactc aagtgtttgc gggacgcaaa ctattatcat ggcctaaatt tttcttgata
    23881 tttgtatttt tagtttcctt gatggtcatg cctgtgactc ttttttatgc caatcagatc
    23941 caagcgattc cattagaaca atttctatcc gttcattcgc tgatcgatga acaaggaacc
    24001 caaaaatttt ctgaacttga attatctgaa actgggttac aagcatcgca acaaacgata
    24061 gctgtgaccc ctgaaatttt ggtaggtgtg tctttatctg agaaacaaca atccgatcat
    24121 ggaactttta ttgattttga gaaggaacaa tgggtgatcc aacaaaagga taaatctggt
    24181 atacgccgct atacgatgaa ttatagccca tctttccaac cagattctgt acgtacgccc
    24241 gaagatttcc aacgattttt agaaagagaa ttttatgcca gtaatcgtcc aacaatcatt
    24301 ttatcttata gtttaagtct tggtcttgtc ctatttgtca tgacgagtct cattttattc
    24361 ggggcttcct ttttcttgtg gatgacaaga aaaagccaac tttcatccat tcatacattc
    24421 aaagagagtg caaatttgat gctgaatgtt atgggaatag gttccatggc agcagcagtt
    24481 gtagggttta tccactttga ctttatcttg atgctaagtg tccaaacaac gataactgtc
    24541 ttgctgcttt tatgggtttt tgtcaaaaca agattcaaag ataagcgtgt cgaatgacaa
    24601 gtttaagcga tttatgcgac aatgaatgtg aaaaaaatga aaaataaagt agtttttgaa
    24661 aaatttctga aaaaattaaa cttcttagtt gattcttttc ttttttagta ttaacgtaat
    24721 agggtagttt ttttaaggag gaaaatcatg ttagagaaaa ctttatacaa tcaattattt
    24781 tcacgttctt tctcgctccc tgtcgaagta aattattggg acggtacaac gaaacaatac
    24841 ggagatacgg acactcctcc ccaaatcaaa atcaatatcc atgaagaaat cccaatgaaa
    24901 gagattacaa gtaacgcttc tttggcacta ggagaagcct atatggatca tcgaatcgag
    24961 atcgagggaa gtatccaagc attgatcaac gatgcttatc agcaagcaaa cagcttttta
    25021 cgaggaaaag attatctaaa atggctgcca aagaaagaaa aacatacgaa acaaaaaaac
    25081 aaagaagata tccatgcgca ttatgactta ggtaacgact tttataaact ttggctagat
    25141 ccaactttga cttattcttg tgcttatttc aatacacctg aagatacgtt ggaacaagca
    25201 caaatcaata aagtccatca tatcttagac aaactcttta tcaaagaagg cgaaacgttg
    25261 cttgatatcg gttgtggctg gggaacattg atgttcactg ctaccaaaga gtataatgtc
    25321 aaggcaactg ggatcacact aagtgaagaa caatacgatt tgattaccaa aagaataaaa
    25381 gaagaacatt tagaagataa atgccgtgta ttattaatgg attaccgcga actaaaaggc
    25441 gaaacgtttg atcatatcac aagtgtaggt atgttcgaac atgtaggttc tgaaaatctt
    25501 gaaggttatt tcaaagtggt aaaagattta ttgaaaccaa aaggaacagc tttgatccat
    25561 ggaatcagcc gacaacaagg cggtgcgaca aatgcttgga tcaataaata tattttccct
    25621 ggcggttatg tccctggtgt tactgaatta gttgataata ttacaaaaaa cgatttgcaa
    25681 ctgattgatt tggaaagctt acgtcgtgac taccaattga cattagaaca ttggacaaag
    25741 aattttcatc aagtccaaga tcaagtcacg aaagaaatgg gtgattcctt ctatcgaatg
    25801 tgggatctat atttacaagc ttgtgcagct tcatttgaat caagtaatat cgatgtgatt
    25861 caatatttgc ttgttcaacc tggaaacaat gacatcccaa tgcatcgtgc ataattgagg
    25921 caaaagtact gtttaaagac tatttaaaaa gaagctaagt tttatgatgc tccagaagtg
    25981 aacttttttg tctaacttct aggattgtct ctaaagctta gtttcttttt ctttatattt
    26041 tcataataaa agtttggttc tttaattttt aaacatgaca gaaaattttt tcttcgttat
    26101 acttgtaata gaatactatt ttataacaaa ggggatcgaa tcaatgaaaa aaactgttat
    26161 ttatactagt ttatttgcag ctttgttttt agctgcttgt caatcaaata ccaatcaagg
    26221 cacatctggg aatacgtctt ccagtagcga acagcgctct tcacaggtat cggactcctc
    26281 aacaaaagca agcacatctt cgtctagcca gacaaaaagt tctagtgaag caagtactca
    26341 gtcagatatc aaagtgtctg cagctcgtgc gatcgaattg ttccaagata aatacccaga
    26401 tactgcaatt actagtttgg aattagatag tgattgggga tcttacttct acaagataga
    26461 aggtgtcgat gatcaaaaag aatacgaagc aagaattgat gctcaaaatg aaaaaatgga
    26521 gacagagaat ccagaaactc tagatcgaga tgaacaaaat ggtcaaaaga aaaaagaaga
    26581 tggtctggat gttgataaat tgatcacgat tgaggaagca gcaaaaatcg ccgtcgacaa
    26641 agtaggaagc ggtacggcaa cagattggga tttggataaa gatttaggta ctacttattg
    26701 ggaagtcaaa gtaaaagacg gcaatcaaag tacatcagtc aagatcaata gccaaacagg
    26761 cgaagtactg tcaactgaac aagatgatta agaatttctt cggaaaacaa gagttgattt
    26821 ttcttttagt catgatacac tagacaaaat aacttgtttg aaggagcgat ttacttgagt
    26881 ttagaaacga cctttgccaa tcatatcttt gccaatcctt tgatgaatgc atctggtgtc
    26941 cactgcatga ctacccagga attagatgaa ctggctcatt cagaagcagg agcatttatc
    27001 actaagagct gtacgatcaa tgaacgaaaa ggaaatcccg aaccacgcta ttttgatgtt
    27061 cctttaggga gtatcaattc tatgggactt ccaaacttag gtttttctta ttatctagag
    27121 tatgctcttg cgtatgaaaa agtgcaggaa aaccaaaatc aaccgttatt tttttccatt
    27181 gccgggatga gtgtccagga aaacttggaa atgcttgaga aaatagaaaa aagtgggttt
    27241 aatgggatca ctgaattaaa tttatcttgt cccaatgtgc ctggaaaacc acaactagct
    27301 tacgattttg aggccactta tgaaacactg aaagaagtat tcagcatctt ttctaagccg
    27361 ctaggaatca aattgcctcc ttattttgat tttgctcatt ttgatcagat ggctgatatc
    27421 ttgaatcagt ttccattgac ctatgtcaat gctatcaaca gtgtgggcaa tggtttgtat
    27481 atcgatacag aacaagaagc agtagtcatc aaaccaaaag aagggttcgg cggcattggc
    27541 ggagagtata tcaagccgac tgctttggcg aatgttcgcg ctttttatac acgtttgaaa
    27601 ccagaaatcc aaatcattgg tacaggtggg attcggacag gacaagatgc atttgaacat
    27661 ctgctatgtg gcgcaagcat gttacagatt ggtacagagc ttcataaaga agggccggaa
    27721 attttttcta gaatcataaa agaattaacg cagattatgt cagaaaaagg atatacgtca
    27781 attgacgaat ttaaaggcaa attacgcacc atttcatagc aaagagacga aggtcatgat
    27841 tgatcttcgt ctttttttat aaaaaacact cgtctgttca ttcctgtgca agcgtactca
    27901 ttgtatgtta gacgaacttg tgggaaaata acagaggcaa aaaggaggat aatatgaatg
    27961 atgcaacaga attagcaaaa gagcttaaag aaaaagaatt tagttttcaa gaactagtta
    28021 cggatataca gaaaaaaata aagaagcaaa atcatgaatt gaatgcgttt gtcgcgcttg
    28081 aaccattcga gagtaattcc cacgagctat tgattcccca gtcagaaaaa gggccattgg
    28141 ctggtatccc atttccttta aaaatgctcg gtcaagaaaa aaagggatgg ctagctactt
    28201 caggatctcg tttatttgaa acacaccgag cttctcatac atctaattat gtgcggcaag
    28261 cagaagcgat aggtctagtt ccttttggac aaaccaatgc gccagagttt ggtttcaaaa
    28321 atattacaga cccagtcatc tatggcccag caagaaaccc ttggaacctt gaatgtacac
    28381 cgggcggatc aagtggagga gctgctgctg cagtttcatc aggaatcgtt cctttagctg
    28441 gtgctagtga tggcggtggt tctattagaa tacccgcttc gttttgcgga ttgattgggc
    28501 tgaaaccttc gcgagggacg atgccagttg gtccatatgc ttggagagga tggcaaggag
    28561 ctgctattga tttcggcttg acagtttcta tgagagacac ggaggcttta ttttatggga
    28621 tgagaagtat ccattccggt gcgccatatc aagtatcgcc agttgagtgg caaacacatc
    28681 caagaaaaaa acggttgaag atagcttttt gtgttgattc accgatccac tcacagatta
    28741 gtgaagaggc agtacttgct gtaaaaaaag ctgtagattt ccttggaaaa gaagggcatg
    28801 atttgacaga gattccttac ccactagatg gtcgtaaact gatcacgtct tattatcaga
    28861 tgaatggcgc agaaacagca gcaatgatcc gctcgattga aacaggcatc cagcgagctg
    28921 tcagaaaaga agaacttgaa cccatttctt ggactttgtt ccaatatggt cagaagctct
    28981 ctgccgctac ttacatcgat tctctgcatg tatgggatga agcagcaatt gtcatggaaa
    29041 acctacatca agaatacgat ctattccttt caccaacgac tgcatttacc gcccctaaaa
    29101 tcagcgaaga tttgcaaagt gatgcgataa gagcacagat ggaacacatc agtgattgtt
    29161 ctgaagtaga aggtttagac gttgtttctt ccatgtttga ggagagcttg aagatcacac
    29221 cttatacaca gttggctaat ctgactggac aaccagcaat cagcttgcct actcatttga
    29281 caaaagaaaa catgcctcta ggaatccaat ttatggctgc cagaggaaga gaagatttgc
    29341 tatttcaaat cggctatcta tttgaagcaa atcatcagtt ccatttacca aatccatgga
    29401 aatagcagaa acaaggagaa gaagaatgaa gaaaaaaaga ttgtttgctc taataactag
    29461 tgcctttttt attttaagcg cttgcggtac agctgccgct aatcaggaag ataacagctg
    29521 gagtaatgta gaaaaaaaga aagaattagt agtagcgact tctggcacac tgtttcccgc
    29581 atcttattat aatgatcaaa atgaattagt tggttatgat gtagatttgg caaaagaggt
    29641 ggctaaacgg ttaaatgtca aaatctcctt taaagaatac aacgtggatg gacaaatcac
    29701 ttctgtttca aaaagagaat cagattttgc tgcgaacgat tttggtatca caaaagaaag
    29761 agaaaaaaaa ttctcccttt cagagacttt caaatattca tttgacagta tgatcgtacg
    29821 aaaaaaagat gattcaggaa tccactcact tgaagatctt aaagggaaaa aagcagctgg
    29881 cgaacccaat actagctata tgcgtttagc ggaaaaacac ggagctaaat tagtcacata
    29941 tgacaatgcc acgaatgatc agtatttaag cgatgtagca aatggtcgaa cagatgttat
    30001 tctaaatgat tattatctgc agaaaatggc cgtatctgct ttaccagata tccctgtaaa
    30061 aatactggag aacgtttatt tcaatgaaaa cgaaagtggt tttttattga aagaaggaaa
    30121 tgatgctctg aaaaagaaaa tcgatggcgt attaaaagag atgaaagaag atgggacgac
    30181 aaagaaaata tctgaaaaat attttcaaac tgatgtttca gtcaaaccga aagaaacgat
    30241 ggctgagtcg gtgagtgagt gatgtacttt cccctaattg atttttccct catgtggact
    30301 tcacttcctt tcgtactgca aggcttggta tacacattag ggattggctt cgtgtcgttt
    30361 cttttaggaa atttggtagg gttgcttttg acagttttag gtcttttgga ttggctgcct
    30421 ctgaatgtat ttattcgttt ttatttatcg ttctttagag gcattcccgc actagtcctg
    30481 ctgtttctgt tatatttcgg gttaccttat caattaagtg ccttaacggc ttcagtcatc
    30541 tgctttacga ttaccagcag tgcatttatt ggtgaaatct atcgaggatc actagcaggc
    30601 gtttcttcag gtcagtggga cgctggttta gcttctggtt tttctttctt tggggtattg
    30661 aaatatatcg ttttgccgca agcttttcga atctcagtac cggcactcag taacgtagct
    30721 atggatctag tgaaaggtac ttcgttagca gcaacaatca cagtgccaga aatatttcaa
    30781 aaagcaaaga tcataggcgg tcggacattc gattattttt ctctctatat tttagttgca
    30841 ctgatttatt gggtcatttg tatcgggatc gaaaaagtgc aacagtattt agaaaaacgt
    30901 tctatccatc agtcaatctg aaaaatagaa atggtgctag cgtggctggc accatttcta
    30961 ttttttgttt cttgtatatt ctgcctagta ttttaaaacc atttcatgta aaatgtaaat
    31021 cgaaaatatg ttcggaggta gaaaacatga agaccccgct aaccatcgaa atagaatcct
    31081 cgttatacca atattgcatc gagcaaggcg gcttggttgt agaagaagtc acgatgcctg
    31141 atgaccaagg aattgtagat acgcttagct gcttgacaaa atttgatgga agccgagaat
    31201 ggcgatgcta tgagctgaaa gtgacaaaag cagattttcg ctctacagcc aaactgtcat
    31261 ttgttggaca ttataattat tttgttttac caagtaaact atatgaagaa gtaaaaactg
    31321 aaatccctac aggaataggt gttttagtgt atcgtccgta cacggttgaa gaggagatgc
    31381 ctgcctctgg tacatttctg attgcgaaaa aagcagcaaa gaaagagctt gctgtaccgg
    31441 aagaagcttt gacaaaccgc ttcatggctt ctttattcag ggaagtaaga aaagcaaagc
    31501 aaatggagcg aggcgtaaaa tattttccaa aagatcaatt gtacaaagaa ttgaaaagac
    31561 gttcagaaaa tcaagatcca ttttctaacg aacgatatta tgaacggttt ttagaggaaa
    31621 tagctagtca ggcaatcaat gagttgcaag aagaagttat cgcgcttcag catgattatg
    31681 aatatttgag gcaacaacag cagatccgtc ggttgcctac agagccattg gagtgaaaaa
    31741 tatgtactat ccttatttaa gaggaaaaca atttgaccta ttagcattaa aagaagcact
    31801 gtcgcgagga ttgctctcca ataaaatcca accagtgatc gaaccagtac gtgattccgc
    31861 tacgcttaaa aatgtgatcg aattatttca aaaaaagcac catccaatcg ctgtgatcca
    31921 aaatccccaa gtaggacaat tcaaattatt cgatcagcat gtgcacagtt ggacagtcaa
    31981 agaaaatagc tctgtcgtcc ctgcacagat cttaacgcca gaaaatctca gtgaagtact
    32041 ggattcacct ccagcgtttc ttgttttcga tggacagcac tacccaaaag atacagaggt
    32101 gtggaaacag ttggcaggag ttgattcgaa atttttgata ccagatacta gtcgctttcg
    32161 catctggctg cccgaaaaca aaattgtgat ccgtgactct tttcaaacga gaaagcatgt
    32221 tgaaagttat gcagataaaa cagatgactt ttttagtgat gactatcttt ttttccacgc
    32281 agacggatat attggatttt ctgatttcac gatcgaaggt agccgttatt tcgataaagg
    32341 gttccctagc cgagcgcttg ctatccatct aacgtatatt gatgcttacg gcaatatacg
    32401 cgtgaaacat tttgtctctg acagtaacga ctctgctaaa gaccaagcat tgaagtttct
    32461 tgaagcagga gataaaatga aaaaatggat catgcgtcac catcatcaac tcttgattac
    32521 ttcaggaatg attgaattat tggctcttta ccagcaacaa aaattccctg gactgggagt
    32581 gttgaaaaag tggtcactta tgcatcattt ggaacttatt agtcacctgc tagatcatcc
    32641 aaaagattgg cttaaatatt attcaaaagt tcaggaacta tatgaagtga tccaatgatt
    32701 agataatgta aatgaaaaag gaatagaaaa gaggaaactg gatgtatatt cggcaaacaa
    32761 ggaaagaaga agtagaagaa gtggtagaaa tcattaatgc cgcagcggat ttcattgctt
    32821 cgcaaggctc tcctcaatgg caaaacgggc agaaaccaac aaaagaaacg attttgaacg
    32881 atattgaaag aaaagaaagc tatgtattaa tagtggaaga tcaaattgct ggaacagctg
    32941 cattagtctc aggcgtggat cccgtttaca caaaaataga tggtagttgg aaagaatcaa
    33001 ctggtcctta tttatcgatt catcgtgtcg ctttagcaga aagtactcga ggaaaaaaac
    33061 tagcaaaacc gttcttagaa catttgttga cgattgcagc cagcaaaaat atccaagata
    33121 tccgtatcga tacttatcca gctaatgaac cgatggaaaa gaccatctat tctgctggat
    33181 tcacttatca agggatgatt gaatttcctt ttgaacacgg cgaaagaaaa gcatatcaaa
    33241 taatcatcaa ttgactataa aatcatctga gcaaagataa agacattaac actactgaaa
    33301 aaaacaatca tatgatagcc aagaggttgt aacagaagtg gccagcttca aaaaacaaga
    33361 aagaatttcc gaaaatgctt ctcaaatttt tgtggaattt tagcgtattt tacgaaatag
    33421 ctgctttctg aaactccctt catcgagagg tcgtgacata gcttttgtca cgatctctcg
    33481 gcttatccta cgagtattat tcgatacatt tatatcaata atcctttttt tattttatct
    33541 gcagcttctt ggaacgctcc atcgtttgac acgactaaaa gtgaggggat agctttcaaa
    33601 cgtttcaact gtgctaaatc ttctacataa accgccagtc tggctgaaat ctgttcagat
    33661 gaatctccac gttttttcag gcgcgattgc aatgtttctt tagagatcgt aataaagata
    33721 ggacggacta ccgtcccgaa atactcatat aaatgccaaa aaccatttgc tgtcaaaacg
    33781 gcgtaacaat ttcctactgt cagtttcttt ttaatttctg ctttactgga accatattta
    33841 tgttgagcat attggtccca ctctgcaaac tcatttccct tgatcatctg attgaatgta
    33901 ttgtcagaaa caaaataata atcctcttga tctttttccc caggtcttgg tgatcgattc
    33961 gtgtaagtaa tgatcttttg ttccggctga aaaatagctt ctgcgagtga agtttttcca
    34021 gaaccagaag gaccgataaa tacatagcaa taattcattg tcatttctcc tttgatgatt
    34081 gaacaagtga aaaagctttg gaaaatattt ccaaagcttt taaaagtaac tttgcttttt
    34141 tgtattttat accagcaagc cagtcacaat agcacttaaa aagctgacta aagtcgctgt
    34201 tagcaaaatt ttcaatccgt gttttgcgac aagatttcct ttttcttcat ttaatccctt
    34261 catcgcacca gaaatgatcc cgatagaaga gaagttggca aatgaaacga ggaagacaga
    34321 tataattgct gttgtacgct cagtaaaatg gaaattacca tttgctaatt ctgtcatagc
    34381 aacaaattca tttgtaacca gtttagtcgc catgatacta ccggcatcca ctgcttcttt
    34441 ccatgggatt ccgctgatga aggcaagagg ggcaaagatg taacctagga tctcttggaa
    34501 agtgatgccg aatatcccgt ggaagatagc attgatcatg gcaatcaaag caacaaaacc
    34561 gataagcata gcagcaaccg tgatcgccac atggaaacca tcaagaatgt attctcccaa
    34621 catttggaaa aatgtctgtt ctttgttttc ttcaatgatc agctcatctt ctttttcttc
    34681 taatacatag ggatttacaa ttgaagccag aatataaccg ccaaataagt tcaaaacaag
    34741 ggcagtgatc acatatttag aatcgatcag tgccatataa gatccgacga tcgacataga
    34801 aactgtagac atagccgata cactcatggt gaacaaacgt ttctcagata ggaagggtaa
    34861 ttcctttttt atggaaataa aaacttccga ttgtcctaaa atagcagaag ctactccatt
    34921 ataagattcc aatttgccca tcccgttgat tttacttaat cctagaccaa cagctttcat
    34981 aaataatgga agaatcttga tatagcgtag aataccgata atagcagaaa taaatacaat
    35041 tggcatcaat acggaaagaa agaaaggtgc acttccttta tttgctacac cggcaaaaac
    35101 aaagtcaaca ccttgcccag caaaagcaag aagatgatca aacatttttg ccaatacaga
    35161 aacaaccgct gtaccaaaag ttgtacgtaa taatacgaaa ccaagaacaa gctgtaaaac
    35221 gaacatcaca attagtggtt tcaatttgat tgcttttttg ttactgctga tcaaaaaagc
    35281 aagccctaat gtgacaaata aaccaataat acctaataaa taactcaaat aatcactcct
    35341 attccaaatt ccgttttatt ttacctgttt tgcaaataaa atactaatct ttagtgaaat
    35401 tttcattact tttttagagt gaaacgcatt cattttccat tagaaaataa agcgaacaaa
    35461 ataaaaggtt taaaaagtgt aacgtcttag taaagtctat aaaaaaacga aactatcgat
    35521 taagctaata gtttcgtttt caacatttat aagtaaactt tttctattgg taatatagat
    35581 tttcggaagg gtagacaggt gcgcaggtaa tatcgattcc tagttttttc aatgtttgtt
    35641 catcattttt attgaccatc acagtagagt gggcttgtgc tccctcaagt tcagctagtt
    35701 tatcataagc aacttgagcg attggattcg ttactgcact gattgataaa gcgatcaaga
    35761 cttcattgat gcttagtgca tccaaagagg aatgaagtgt ctttgatttc aatccttgta
    35821 ttgtttcaag aactaatggt gacaataaag gaatgtcatc tgcgatatgg gctaaatgtt
    35881 ttactgcatt taagataact gccgctgacg catccatcaa tgaacttgta cgcccggtaa
    35941 tgatgacttc atctggtaat tcgattgcaa tcacagcaga aggatctttt gtaggacttt
    36001 gcgcttgaat cttatctgca tagtcatggg cagggactac cacgtttcgg tcttcaggac
    36061 gtaaaccgac ttcttccata atcagtttga ttcgattaac tgcttcttcg tcgatcaatc
    36121 cttttttgaa gtcgcattct gtttggaaat aacgacggat gatttcttgt ttagaggctt
    36181 cttggacaac ggtatcatcc acgatcccaa atcctacacg attcacaccc atgtcagtag
    36241 gagattgata aacagattct ttaccagtaa ttttttcaat aatacgtttg attacaggaa
    36301 atgtctcaac atcccgatta tagttcacag ctactttatt gtaggcatca aagtggaagg
    36361 aatcgatcat attgacatct tttagatcga ctgtggctgc ttcataagca atgttcagcg
    36421 gatgtttcaa cggaacattc catactggga aagtttcgaa cttggagtaa ccagctgcat
    36481 gtcctttttg gctttcatga tacagttggt tcaagcaagt agcgagcttt ccacttcctg
    36541 ggccaggagc tgtcactaca acgattggtt tagttgtttc aatatactca ttttttccaa
    36601 aaccatcttc tgagacgatt ttttctacgt tgactggata gtcttcgatt tctgagtgtt
    36661 tatagacctt gatgtttcgt ctttctagtt tattgataaa gactttcgtg gcaggctgtc
    36721 cactatagcg agtgataacg acactgttga ttaataagcc ataaccacgt aattcatcga
    36781 ttaaccgcaa gatatccata tcataagtaa tcccataatc accacggatc ttattacgtt
    36841 caatatctcc agcatagacg caaatcagta tttcagcttg atctttcaat ttttgcagca
    36901 atttgatttt cgcatcttct tcaaatcctg gaagtacacg tttcgcatgc ttgtcgtcaa
    36961 ctaacttccc accaaattct aaatataatt tgtcatagtg gttcactcgt tccaaaatat
    37021 acgctgactg ctcttcaata tatttttctg aatcaaaacc aatttttttc actaggttcc
    37081 ctccttaagt actaaagcat tttttcattt ttttgaggat tcgtcaactg gaagagcgaa
    37141 attcctcaaa aaaacaaaga aaatggttta aaccaacttg tctagatagt tattcattgg
    37201 tatgatagtt tttgataagg aaggtgctac atgaaaaaaa gacagaaaaa gaaaaacgca
    37261 tataaacact atatccgttc gatttttacc ggttatgaaa gaatgcttga agatcccgaa
    37321 ttggaacaat tgacgtttac ttatctaaac gaagaaacgc agctaactcg tgatgagcat
    37381 cagcgcattc atttcacaac aagagatctg cctagtaaat aaaaaataag ataaaggtca
    37441 tcggattcga ttctgtccta tcagatggaa agactagttg atcgaaaaaa aggctgtacc
    37501 aaattttggt gcagcctgtt ttatggagca ttgcccgctc ttttttataa caatttttta
    37561 cagaaaatgg tactattgaa gtattaataa gtgaaaaaca gatgagtaag aggaggcaag
    37621 tagtatgaaa gagccagata caaatgcaaa ttatgagcca gacgaactag aggagacgaa
    37681 cgcggatcag gatacaggac aagaatcgtc ttctttttac gatatcaaag aaaaagcaag
    37741 catgaatctt tcccaattat tagaagactt acaggctttt gaaaaagctg tccgcgaaga
    37801 agatatccct gagatctacc gtttatacaa tggccgattg aatgaagaat taaaaagaag
    37861 ctcaaatgaa aatcacgaga tcgatcaatt gctgatgcgt aagatccatg atcgtttttt
    37921 acaggaattt ccttttatgg aacaagtaga aaacatctcg ccaacgatga gctattacaa
    37981 aatcggcact tattaccatg atcggcccac ggtcggtatt gatgcaagtc tgccagaaat
    38041 ttttgttttg cctaagatcg atgaagagtg ggagaaatat tcccaaccgg agtcaacgga
    38101 ctatgacaaa aaaatcaatg agctagacgc aaaggtgatc actgctcaga cagaaattga
    38161 acgtttagat gaacaaataa aagaaatcaa tcgacaaatg actgatttga acgacaataa
    38221 aggattcttg aatcgtaaaa aaatcgagga agaaattcaa gaattagaga agaagaagca
    38281 agtactatct aatgaaaaat taggctggct tccttatatt gagacgcctg aaacaatcca
    38341 gcagcaaaaa gaagcattga agcaagaagc aagagcagac cagcttcgtg cagccatcgt
    38401 agaaaaagaa caaaggcaaa tcagacgcta tttcggtagc aaagagggat tcggtcaagc
    38461 aatccatgaa ttcttgatga attatcttgg caatgagaat cctaataatc aatctaatga
    38521 aggaggttcc gagtatgaat gaacacgatg aagcagaatg gacaaaaaat gaagaacaaa
    38581 cagaagatca tgaagaaacg aacacttcat tggaagaaca aacaagtaat cctctggatg
    38641 acacagaaga aatccctatg ctagaggaaa caaaaataca tgaaaaagaa aaagtaactc
    38701 cgacgaatac gaatactgca gaaaacaccc atgcttacga aataactgaa aatcccgggt
    38761 atttagaagg aaaagagcaa gatcatcaag atatcgtcag tttgattgaa acagcggaag
    38821 cagaattagc ggatttacgt atgagaaaag atttgatcga atcagatttt ccacaattat
    38881 tagaatcata tgatcgtttt atattgaatg aagaaccaat ctccacgatc ggaaagacaa
    38941 gcctgatcga atacttcacg tatgaattac tgaaaccgtt gaatgatatt ggtctggaac
    39001 ggtcaaattc agcttatgac acatggaaaa caagacattt ttcgatcaat tatacgttga
    39061 atggacaaaa cgaattagtc ttttcctttg tattaccgac catcgatcaa cgataccaag
    39121 tggaatcgat gagtctccta aacgtatcac cagagacaat ggaaatcaat gtccaagatg
    39181 accgggtgct ttctttgatc cgacactggt cagtggatcg agtattctct gcagggcaga
    39241 tcacgatatt caatcacaaa ttgaatcaat tattagccca tgcaagaaca cttggtttta
    39301 ccgttaatca gacattacta gacaatacga agccacttcg tttgaatctt cagagcgaat
    39361 ttgaattgac gaatgaagta ttggatgata tttttatcgt ggcaatgaaa aatccatcct
    39421 acgatatgga aaagatcaac gaaactactt atcaagtacg attagataaa ggacagtctt
    39481 taacgataac aacaaacgaa aaagatcaaa cagtcttgtc gatctcatct ggagactacc
    39541 cacgatctgt cattgatttc tttatcaatt acgaattctt ggtcccatta atggtgagaa
    39601 agtcctgatt ggataagttg gattaccaga tgtcattcag ataattctgt tccaaaggac
    39661 ttgcggcagt tatccagaat aattgtcaca agtcttttct gtatgtacta aatggacgtg
    39721 aataacttgc tgatgaagga gggatttcat taggaatgtc cacaaataaa atagcacagc
    39781 ttagcttatt gtctgctgct tgtgtagcag gtaggatcgc ttttcaattt attccgaatt
    39841 tccagccaat gactgctatt tttttattta tagtacttta cttaagctta aaagatgcgt
    39901 taattgtcat gagtctttct attgcgatca gcagttttta ttttggtgtt ggaccatggg
    39961 tcatcggcca atggatcagc tatactgttg ttctgagttt gttttcaatc gtctgcttga
    40021 gaaaaacagt gcaaaggaat ctatggttaa agggagtctg tttttttctg gcgggcatgg
    40081 tttacggaat aggtatgacc gtttttgata cgctgttata tcgtttgcct caaccttgga
    40141 tctattacct tcaaggggtg tcgtttgatt tgatgcacag tataggcaat gttgtgttct
    40201 ttcttgtttt tctgccagtc gtcaaacgtt tttacaatca ttcaggagga aaaaatgaaa
    40261 aaaataattt atagtttgct tgcaatgagt gtcagtatac tcattttgac aagttgcagt
    40321 acaacctata cacaatcggc ttccaaccaa aaggaggaag taaaaacatc gcaaaaagca
    40381 acaatcacgt tacaagaaaa tggtcaaaac ttttcggaaa aagaagtggt cttcaaaaaa
    40441 ggcgatgatt tactgagtat tttgaaacga aattttgaag tcaaagaaga taacggcttc
    40501 atcacttcgc ttgaagggca ctcacaggat gagaaaaatc aactctattg gatgtttact
    40561 gtggacggga aaagtgctac tactggtgcc aaagagatca aattgaatga tggacaatcg
    40621 gtcattttca atttgtctaa actataagtt tcataaaaaa ctgttaaagg gcgaatattg
    40681 ccataattca gtgagttcag ttgatgaaag aaggaaaata acatggaaaa ttttaaaaaa
    40741 agcggaatgt tttccgtaat tgctgctcta ggggctaata ttctagttgc tatcagcaaa
    40801 tttgtcggct atgcaatcag tggcagtgcc gccatgctaa acgaaagcat ccacagtatc
    40861 gttgattgca gcaatcagat tttgctgtta ttcggtgata aacaagccaa taaaggacag
    40921 agcgagttgc atcaatttgg tgaaggacga gcaaaatact ttttcagtac aatcgtcgca
    40981 atgatgcttt tcttcggcgg tggggcactt ggggtgatgg aagcttttga aaagttgacg
    41041 catccatccc atgaagtggg taatccgatc attgttatag caatactgtt atttggtttg
    41101 gtaatcgaag gaagttcgtt acgcgtggca atgaaagaaa tcaaagaatt gaatactgaa
    41161 aaactttcca cgatgcgctt tttgcgcgaa agtcgacaca gtgaaatact gattattttt
    41221 acggaagact tgtgtgctgt aatcggtttg gtcttggctt tagcaggaac aatattgact
    41281 tatgcaaccg ggattgctgc atttgatgcg atcagcggat tgctgatcgg tttcttgctg
    41341 atgggagccg ccattttcct agcaaaagaa ttttatagtc tgatcatcgg tgaaagcgtg
    41401 acagcaagcg atttatcaaa aatcaagtta gcttttgaac gtcctgagat cagtcggctg
    41461 atcgatataa aaacagttca tttaagtcca acggagattt tagttgcagc aaaagcagat
    41521 atcgttggta acaaagagaa tgaagcttac tcggtcatca atgatattga aaaaaatatg
    41581 cgtcagtctc ttcctgacaa gaagatgtat atatacattg aaacagataa atatgaccca
    41641 aattattctc gtaaataaaa cgtttattca tgaattgaag caaaaatgag aaaaatataa
    41701 tcttgttttt aaataaaacg gctacaattt agcgaattta tgttacaatg agtccgctgt
    41761 ttatttgttg taattaaagg agatatttta atgaacaaaa agaaaagtgc gccatggctt
    41821 caagctttgc atgcagtgat gccactttgt ataagctata taccagtagg attagcttgc
    41881 ggagtattgt tgcaaaaagt tggatttgat cctctcttat caggactgtt atcgattctg
    41941 gtcttttccg gtggcgcgca gtttttagtt gcatctatgc ttacgacgca agcatcattt
    42001 gcaacgacat tattgatggt tttcttttta gaattacgct atacattatt aggagcaagt
    42061 cttgccggtt ttatgaaaaa agaaaagcgg acgtttttgg ctgtcttttc tcaatcactg
    42121 aatgatgaaa actatgcggt gaactactta aagttttcaa ctgacccttc ctggaataaa
    42181 agaaaggctt tatatgtcaa ttggttctca atgactgcat gggccggtag caatatgatc
    42241 ggtaattttt tcggctcggt gattcgagta gatgctgacc ttgtccattt tgctttgacg
    42301 gccatgttca ttttcatgtt tatcatgcag atgaaaagcg cactactgat ctttacaggt
    42361 ttattctcag gcgtattagg ggtcgtattc atggtattat tccaaaatac tttcggattg
    42421 attgcagcaa cagtcattgc ttcatttgcc ggatttatgc tggagaaggc attcaaaaaa
    42481 gaaaaagcta agaaactaag agaccgcggt aaagatgtca atgagccgct cttcaacttc
    42541 ccggaacagg aggccaatca ccatgactaa actatatttg ctacttttag ttgcttgtct
    42601 gttcatcgtg gcatatatcc cgcgcttgtt cccgatgctt tattttactc atcgaaaagt
    42661 gccatcctgg ttcagtgact ggatgaaata tgtaccggtg gcattatttg cggcgttagc
    42721 ttttaaagat gttttcatta ctcatgaaca tctggatatt gcttggaata tcaaaatagc
    42781 tgcaatgatt ctagtagcag gcgtggctta caaaacacgt tcgatggcgt tgtcagtttt
    42841 gacaggactt gcgtctgtct ttttgctgtc tatgctttaa actgttttat tttattaaaa
    42901 acagagattg cgacataagt tgttcaaact gatctatccg aactgcattt gcaacagtca
    42961 attctggatg caagtttatt cgtagggata aaaaagatta gtgttggctt atgatgcaag
    43021 ctctttttta ttttaggaat tagagaggga tttgtccttc tatgattttt tgctataata
    43081 gggaagatgt ttattttatg aaagaatagt tgagggaatt taaatgaata aaaaattgat
    43141 gcaaggaaca ttttggctaa cctttgctaa tcttctatgt aaagttttag gggtagtcta
    43201 tttgattccc tggctaagta tgatggggaa caatcaagat ggtatgcttg ctacgacatt
    43261 gtataatgtt ggctatcttc cttatggatt gttcctgatg cttggtacag tcggatttcc
    43321 aaatgcgatt gcaaaaaaag tagctgtagc cacaaaagaa ggagataacg ctgcttgtcg
    43381 gctgattttt agaagtacga tcaatatcat gtttgtcatc ggtatcgcct ctgctgcact
    43441 gatgtattta tttgctcctt tactagccgg aatcagtccg attgctaacg ttaataatgg
    43501 gatactagct atcagaagtt tatgcccatc tttgattgct atcccaattc ttagtgcaat
    43561 gagaggatat ttccaaggaa aaaatgatct gcggccttat ggaacttctt tgattatcga
    43621 acaggctgtg cgagtgatcg ttatcttggc tggtacttat tacttgcgcg tattgactga
    43681 cggaaccata ttagaagctg tattgatcag tacagtcgct tctttcttcg gtgggttagc
    43741 tgcgattatc catatgtata tcgtcggacg cagaaaagac ttcttcgaac ttaaagattt
    43801 tttgatttcc agccgttatt tcgaaagaga aaaccgatct gcctctgtct ctatcattcg
    43861 ggaaacacta ccgtttattt ttgtcggctc agtcattact atcgttcaaa tgatcgacca
    43921 agtaacgatg aaaccactgc ttcacttttt ccgtccagag atcgcagatc agcaactgga
    43981 attcttattc agccgtgctt ctgtcaatcc aaataaattg acattgatcc tgatctccat
    44041 ggtaggaaca gtagctataa gcagcttgcc aatattaagc acgatgaaga aaaaagatcg
    44101 gatacaaatc gaaaaaacag taggagatag tttttcgatt gcgttattga ttcttcttcc
    44161 ttcattgact ggaatgtctt tattagctgg accactatac acattgtttt ttggctatga
    44221 tcctgaaagt gtcggatatt tccaaatggc tttgctagct tctttgtttt tctctttatt
    44281 cacgatccta tctacgatgc ttcaatcttt gaatcaccat cgttttgcga tccgattaac
    44341 tgtagaagca attatcctga aagtcatctt ccaagtaatc ggattaggat tagttggcgg
    44401 atacgggatg agcttatcaa atggcttagc attcggtatt gtctttgttc gcggctatca
    44461 ctttatgtgc aaagaatacc gaatagcacc tttagcaaag atcagcaatt ttttcttaaa
    44521 aactttccgc tccaccctga ttatgcttgc agtatgtttt gccgttttct ttttgctgaa
    44581 tcagcgattg tcaatggaat caaaaagcta tgcaatgatt tattgtgtgg ctgtaggaag
    44641 tattggcggc ttagtatttt tattttctca attcggaaaa aatgggtttc gaatgttgaa
    44701 aaatgttaaa catcatagca gaagcaaaga ataggttaac taatcgtttt ttgctgtcac
    44761 ttgaaaaacg gcaactcgta tgattacgct tgtcgttttt ctattttcca attttagttt
    44821 catatctaat cttacttatg atatagttat aaggtctgga aaaattcttt gaaacgagga
    44881 aattaaacac atgaaacttg cagtagttac agatagttcg gcatatctgc cggaacgtat
    44941 caagaaccat cccgacttat tcgttattcc aatccctttg attatggatg ggaaaattta
    45001 taacgaagga atcgatatcg aagcggatga atattatgga cttttaaaaa acagtaaaga
    45061 ttttcctact acttcacaac ctgtcgtagg ggaagttttg ggtttatatg aggaattgaa
    45121 agagaaaggg tatgacacga ttttaagtat ccatctttct tcagggattt ctggctttgt
    45181 caacacatta tttgcgatga aagatgacat cagcggagta acggtcattc cttatgactc
    45241 taagatcact agtatgccga tgggacacat ggtagaagct gccttagact taaatgatga
    45301 aggtcagtcc cttgatgaaa tacttgcaca tatcgatcga atccgagata atacctatgc
    45361 ttatctgatt gtagatgatt tgaacaattt agtacgagga ggacgtttga caaatggtgc
    45421 agcgctgatt ggcagcttat taaaaatcaa accgatcctg acattttctg aagggaaaat
    45481 tgttttattc gaaaaaatcc gttcaagtaa aaaagcattt gctcgagctg aagaaattat
    45541 tggtagaaga aataacgaaa tacaaccgcc agtcaaactt tacgtgatac atgcaaataa
    45601 tttagaagta gcgattgcgg agaaagagaa attgcagaaa aaatatccaa atgcagaaat
    45661 cgaaattggt catttcggac cagtcattgg tacacattta ggtgaaaaag ccatcggcat
    45721 tgccatttca gcgcaataat tccttttatt caaaaaacat accaaaaata
//
LOCUS       DAZKLM010000012        45484 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3764
            SAMEA115706913-rid22582563.denovo.012, whole genome shotgun
            sequence.
ACCESSION   DAZKLM010000012 DAZKLM010000000
VERSION     DAZKLM010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706913
            Sequence Read Archive: ERR13196647
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 45484)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45484)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287823.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287823.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,004
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,849
            CDSs (with protein)               :: 2,849
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 41 of 96
            Pseudo Genes (incomplete)         :: 52 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 15 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45484
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706913-rid22582563.denovo.012"
                     /isolate="VRE3764"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(56..1691)
                     /locus_tag="ACG842_000606"
                     /pseudo
     CDS             complement(56..1691)
                     /locus_tag="ACG842_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294751.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(2014..2493)
                     /gene="rlmH"
                     /locus_tag="ACG842_000607"
     CDS             complement(2014..2493)
                     /gene="rlmH"
                     /locus_tag="ACG842_000607"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HFQ0247169.1"
                     /translation="MNIKIISVGKLKEKYLIQGINEYVKRLNAYAKIELIEVPDEKAP
                     ENLSEAQMRQVKEKEGERILAKIKEQEYVYALAIEGKNPTSESFAKQIDQLGIQGKSH
                     LVFVIGGSLGLSEAVMKRSNAQISFGKMTYPHQLMRLILVEQIYRAFRINAGAPYHK"
     gene            2921..4210
                     /locus_tag="ACG842_000608"
     CDS             2921..4210
                     /locus_tag="ACG842_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HFQ0247170.1"
                     /translation="MDRKNVTPKMKKSKNNSLWRKLGLGLVGGIVGGLLTAGIFYAAM
                     GTGNNSSTATSGNQNSAGETVVENVKVNVDSDITKAVDKVQDAVVSVINLQSQNQSSG
                     FGGLFGQQEESSSSDDSNLEAYSEGSGVIYKKDGNTAYVVTNNHVVDGQQGLEVLMKD
                     GTKVKAELVGTDAYSDLAVLKINSDKVETVASFGDSSALKVGEPAIAIGSPLGSEYAN
                     SVTSGIISSLNRQVTSTNESNETVNINAIQTDAAINPGNSGGPLVNIEGQVIGINSSK
                     IASTSESTSNVSVEGMGFAIPSNDVVNIINQLEKDGKVTRPALGITMVDLSAVSTQQQ
                     EQILKIPSSVTNGVIVTNVQTATPAEKAGLKQYDVITKIDDKEVSSGVELQSALYKKK
                     VGDSVKITYYEGSKEKTTTVELTIDQSALKQNQNSEN"
     gene            complement(4249..4854)
                     /gene="ytpR"
                     /locus_tag="ACG842_000609"
     CDS             complement(4249..4854)
                     /gene="ytpR"
                     /locus_tag="ACG842_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtpR family tRNA-binding protein"
                     /protein_id="HFQ0247171.1"
                     /translation="MIFAYNKAHVGDTLMVIIADDKGTENTVERKWNVARIKDEQGQV
                     VAWNFFHISDHLTIEGNGQVSLTDEELAKLNRLIKEAGFEEQLEADHEPKIVVGYVKT
                     CVPHPDSDHLSITETEVDNGTVLQIVCGAPNIEAGQKVVVAKPGAMMPDGMMIWPGTL
                     RGVDSYGMICSARELHLPNAPAKKGILVLPEDAVVGEAFPK"
     gene            complement(5059..5523)
                     /locus_tag="ACG842_000610"
     CDS             complement(5059..5523)
                     /locus_tag="ACG842_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HFQ0247172.1"
                     /translation="MNQEYKNILVGIDGSEQALAAFKKAVEVARRNNGKVYVANVIDQ
                     QFYNFMGYAPIDQNLVDQQTETAKEMIEECKEYGKSVDYTNIEGIIAYGSVRESMAVK
                     LPKKYEIDLIMVGQSGLNAVERFMTGSVASYVIRHSPCDVLIVSDEEEQPSN"
     gene            complement(5545..5865)
                     /locus_tag="ACG842_000611"
     CDS             complement(5545..5865)
                     /locus_tag="ACG842_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HFQ0247173.1"
                     /translation="MIIPNSLEELAGYVETGKSVFFFTAGWCGDCRFIKPQMPEIEND
                     FSDWQFIEVDRDKYIDVAAEWNIFGIPSFVVIQDGKELGRLVNKDRKTKQEIESFLES
                     VTKG"
     gene            complement(6009..7088)
                     /gene="pepA"
                     /locus_tag="ACG842_000612"
     CDS             complement(6009..7088)
                     /gene="pepA"
                     /locus_tag="ACG842_000612"
                     /EC_number="3.4.11.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl aminopeptidase"
                     /protein_id="HFQ0247174.1"
                     /translation="MEEKTFQRIKELTELQGTSGFEQDVRAYMESHMEPLVDELQLDG
                     LGGIFGLRHHEEADAPRVMVAAHMDEVGFMLTQIQENGLFKVVPLGGWNPYVVSAQRF
                     TLKTSTGKNYPCISSSVPPHLLRGTSGQKSVEVTDILFDAGFESREEAMSYGVLPGDT
                     IVPYAETIKTANGKNIISKSWDNRYGCTMVLEALEALQNETLGHTLIAGANVQEEVGL
                     RGSKASVNKFKPDLFFAVDCSAADDTVTKNGTFGHLGEGTLMRIQDPGLIMLPRLREY
                     LLDIAETNNIPYQYFVSKGGTDAGAAHTQNEGIPSTVIGVVGRYIHTHQTMFSIRDFE
                     AAREMLIQTLKGLDKSTVNTIVYGK"
     gene            7288..7638
                     /locus_tag="ACG842_000613"
     CDS             7288..7638
                     /locus_tag="ACG842_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HFQ0247175.1"
                     /translation="MYEESELSYFKGGLAVGIGLGLLSGVASTIWYHRKKVLSADQVL
                     ENIKSSFLKEGAIEGSWISFEKEPIRKFAVRSSAYRGGLSRIEDGETVYYEFLADAHT
                     GTVLEISRKKGATD"
     gene            7861..8808
                     /locus_tag="ACG842_000614"
     CDS             7861..8808
                     /locus_tag="ACG842_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFQ0247176.1"
                     /translation="MEQQEKIAVLIPCYNEEATISTVIADFKRELPEADIYVYDNNST
                     DRTYELAVQGGAIVKKEPRQGKGNVIRQMFFDIDADYYLMVDGDDTYPAEAVHGLLEK
                     LRSGEADMVIGDRLSNGTYFDENKRPFHDFGNNLVRNTITRMYKTKILDVMTGYRGFN
                     RIFVKSFPIMSSGFQIETELTIHALDKKFKFVEIPIDYRDRPEGSESKLNTFSDGFKV
                     IMMIIKMFKDYKPLLFFGIWTIFFFLFGLVTGIPVIREYMLTSFITRIPSAILSTGLM
                     TLALLSLVTGLILDTVVTNAKKEYELNLYHVYHEYHSRK"
     gene            complement(8865..10283)
                     /gene="pepV"
                     /locus_tag="ACG842_000615"
     CDS             complement(8865..10283)
                     /gene="pepV"
                     /locus_tag="ACG842_000615"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HFQ0247177.1"
                     /translation="MTIDWQKEVEARKEDLLEDLKNLLRVNSERDDSKVTPDAPFGPG
                     PRDALKHMLAYGERDGFKVKNVDNYAGHIDLGEGDETLGIFGHMDVVPAGDGWDTDPY
                     EPVIKDGKIFARGSSDDKGPSMAAYYAMKIIKELDLKLSKKVRFVVGSDEESGWGDMA
                     YYFEHEEEPDFGFSPDAEFPIINGEKGNVSLALRFKGDNAGDYVLKSFVSGLRENMVP
                     GTATAALQVPSADAAIAMEEAFYQFIEANPVSGTIEADNTYVKIELVGKGAHGASPQS
                     GINAGSFLALFLDNYEFLGSAKQFIHVAAAYVHEDFYGEKLGVAYEDEKMGKLTMNAG
                     LFAFEENGTEEANFINMNFRFPKGVTVDGLQSDIEQTVGQEGATVTRGARVMEPHYVP
                     MDDPLVATLLQVYEDHTGEKGYEQIIGGGTYGRLLKRGVAYGAMFPGYTDTMHQANEF
                     MSLDDLFRATAIYADAIYRLAK"
     gene            10432..11277
                     /locus_tag="ACG842_000616"
     CDS             10432..11277
                     /locus_tag="ACG842_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HFQ0247178.1"
                     /translation="MLINEQLVKRVITPRPKISHKGTFGRIVLIGGNEQYGGAIIMSA
                     LAAVHSGAGLTTVVTDEKNRSALHSHLPEAMIVVWTDTDQFLSVIKQADVLLIGPGLG
                     TSSKSLERFKQVLENQSKEQWLVIDGSALSLLAANHLLLPYPEQTVLTPHQMEWQRLS
                     SLPIQEQRDKENKKKQQEIGAVLVLKSHETKIYGHQHFYTNPLGSAAMATGGMGDTLA
                     GMITGFLAQFPQNDQTIAAAIYLHSFIGDQLAKEQYIVLPTQISEALPKWMNYFCKKS
                     MENEA"
     gene            11281..11721
                     /locus_tag="ACG842_000617"
     CDS             11281..11721
                     /locus_tag="ACG842_000617"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HFQ0247179.1"
                     /translation="MEKFTGYVREPFYYETDQMGIIHHSNYIRWFEEARVALLVHLGF
                     PYKKIEESGIIIPVLEVNCSYKEMIHYGEKVSIHPTIQKYTGTRLDFSYEIVGTEDKK
                     LKTTGSSKHCFLLSDSQRLVKLSKVNPELDQLFQAYASLDDVNS"
     gene            11771..11965
                     /locus_tag="ACG842_000618"
     CDS             11771..11965
                     /locus_tag="ACG842_000618"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0247180.1"
                     /translation="MKRKKQVIPMYVVKVMHGYIDKTGCRTREKNLDNLLIFKDKKES
                     EAFAKRIGGRVKPIQEVRPD"
     gene            12101..12787
                     /locus_tag="ACG842_000619"
     CDS             12101..12787
                     /locus_tag="ACG842_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFQ0247181.1"
                     /translation="MVINNLQQQAYESIRKQIIYSELAPGSKISDKVLEEQLAIGRTP
                     IQEALIQLRNQELVKTIPQSGTYVSKIDIRSASLARYTREKLEKPILQECSAKMTKQD
                     QAKLEKILEQTDQAVIAQNKKAFFDLDTDFHRTCYEIAGKREIWDWLESYSTHLNRFR
                     WLRLTISELDWGRVLDEHQTMLQSMIDHNFDEVGFLCTMHLHMIIEEQEYVIHNYPDY
                     FEDIQDRPIK"
     gene            complement(13195..13884)
                     /locus_tag="ACG842_000620"
     CDS             complement(13195..13884)
                     /locus_tag="ACG842_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0247182.1"
                     /translation="MPEISNKHKLEELLNELQQIPEEIWGFYQFRRDLFWKKIPLSKQ
                     KILIQQSIDCGIETACSIKKKYPFADVGEICEQMAVPIVSCESEQINERITFATYAED
                     EGIRLMTEPLEKLKCSGLASISKETAQALIIGHELFHHIEASVKGIYTQSEKIVLWRL
                     PFYTHQSNIRALSEIAAMSFSKEMNQSDFSPYVLEAVLLWPYNEIRSQGILEEIKKIE
                     KRCTEYDLDYK"
     gene            complement(13859..15988)
                     /locus_tag="ACG842_000621"
     CDS             complement(13859..15988)
                     /locus_tag="ACG842_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HFQ0247183.1"
                     /translation="MKRFVRMGIDVGGTHTKAVAIDNATHEIIGKSSVKTTHDDVRGV
                     AAGVVQSFQNCLLENNISPEDVVFVAHSTTQATNALIEGDVAKVGVIGMAKGGLEGFL
                     AKRQTRLNDIDLGNKKKIEIVNAFLPVKHLNVDRVSETISSLERERAEVLVSSMAFGV
                     DNGEPERVVYEAASVKSIPTTMASDITKLYGLTRRTRTAAINASILPKMLDTATSTED
                     SVREAGVNVSLMIMRGDGGVMEINEMKKRPVLTMLSGPAASVMGSLMYLRASNGVYFE
                     VGGTTTNIGVIKNGRPAIDYSIVGGHPTYISSLDVRVLGVAGGSMVRANQSGIIDVGP
                     RSAHIAGLDYAVFTETEKIKGPKVEFFSPKEGDPADYVKVVMEDGEEVTITNTCAANV
                     LGLVQEEHFSYGNVPSARKAIQALADYCHTTVEDIAEQIMEKSYAKIEPVILELADKY
                     HLEKDQISLVGVGGGAASLITYFSNKMGVKYSIPENAEVISSIGVALAMVRDVVERII
                     PSPSKEDIRSLKNEAMNKAIESGATPESIEVHVEIDPQTSKVTAIATGSTEVKATDLT
                     KEITTEEALELAAEDMRLNKNEVCLLENTPFFYVCGEQNRSKNAGSLRIIDQKGFIKV
                     QRGHASCMKTTAANYMTAVEQLWEDMAVYQTELIARPEFYLCLGARVSDFTATDLEQL
                     QLLMDLEVSTMEPEEEVIVVAGNIKQT"
     gene            complement(16036..17373)
                     /locus_tag="ACG842_000622"
     CDS             complement(16036..17373)
                     /locus_tag="ACG842_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate transporter"
                     /protein_id="HFQ0247184.1"
                     /translation="MSDIIIGILLVISFFFMVWYCVKGYNLMVGFAIMATVWMGLALV
                     GNTFSPNPAMEGQGVIDVLTHIYTTGPAEYAKSILVNVFFGAFFGRVLVDSGIAATLI
                     RKVVELGGDKPRITMSLLCIVTAVIFMSMTGIGPVISIAVIVLPILMSLGISVPVALF
                     SFMGSIMAGIFANIVNFKQYQTIYAGFNPAAESYTYNDYFQIGMIGMIVSLVVVLTVA
                     NISMNKKKRYAMAANVPAEGGDAPMISWLAVLLPVLGVVLLDLPIILGFILAGIWALL
                     FTGKLRGGYKEICRQFAKLFTDGAVDVAPMVGFLMTLAMFNNSAAYASPYFSAIFGDW
                     IPQSPLVLAIVFAILTPLGFFRGPMNLVGSGSAILAVVLAVNPTMSPAFLFPLFAITT
                     IAPQHLDITQSWVAWGLGYTKVTSREYMKKSIPTGWIIGAILCLITFLLYGNA"
     gene            complement(17625..17984)
                     /gene="rplT"
                     /locus_tag="ACG842_000623"
     CDS             complement(17625..17984)
                     /gene="rplT"
                     /locus_tag="ACG842_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HFQ0247185.1"
                     /translation="MARVKGGTVTRKRRKRVLKLAKGYYGSKHTLFKTAKEQVMNSYN
                     YAYRDRRQKKRDFRKLWIARINAAARMNGLSYSKLMHGLKVAEIDINRKMLADLAVND
                     AAAFTALADQAKEALAK"
     gene            complement(18037..18237)
                     /gene="rpmI"
                     /locus_tag="ACG842_000624"
     CDS             complement(18037..18237)
                     /gene="rpmI"
                     /locus_tag="ACG842_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HFQ0247186.1"
                     /translation="MPKQKTHRGSAKRFKRTGKGGLKRFRAFTSHRFHGKTKKQRRQL
                     RKASMVSSGDFKRIRQQLAKMK"
     gene            complement(18309..18833)
                     /gene="infC"
                     /locus_tag="ACG842_000625"
     CDS             complement(18309..18833)
                     /gene="infC"
                     /locus_tag="ACG842_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HFQ0247187.1"
                     /translation="MTIAKDMMVNDGIRARELRLIGSDGEQLGVKTKAEALNIAEQAN
                     LDLVLVAPGAKPPVARIMDYGKYRFEQQKKDREARKKQKVINVKEVRLSPTIDLNDFN
                     TKLRNARKFLEKGDKVKASIRFKGRAITHKEIGQKVLNRLAEETADIATVEQKAKMDG
                     RSMFLTLAPKNDSK"
     misc_feature    complement(18877..19002)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00558"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ribosomal protein L20 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00558"
     gene            19153..19785
                     /locus_tag="ACG842_000626"
     CDS             19153..19785
                     /locus_tag="ACG842_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HFQ0247188.1"
                     /translation="MKKIEPIPPSLPLLTKGEFYLEDEEIFSGLLINGGDQSYLDAKN
                     MVLRESHIQKLTMQKTVLRHFECSNVIFEKCDFSNLEWIGGSFHQVVFHQCKLTGTNF
                     AESYLRDCTFTDCIASMASFSSTNLKAVSFDHCQLEDSEFYEVTWKNLFLSDNQLTGS
                     NWFRTSLKGLDFTTNFFTAIALSQDSLAGLIVNQEQALVIAQALGLQIKD"
     gene            complement(19787..20692)
                     /locus_tag="ACG842_000627"
     CDS             complement(19787..20692)
                     /locus_tag="ACG842_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HFQ0247189.1"
                     /translation="MREKHYFNQDRNFWINVQQNDVLAVKNLKEEYGISDEMLTYSLD
                     KNERARVEYDSFDEALLLVSNVPHQQKVDNHYETSPIAFILKEDGLFTFTTPNTEYVI
                     RLIRSLLERMPDMSVYSLLFRTLFLISDSFFPLIEEVNSERQRLNLKLREKTTNKNLL
                     QLSDLEIGLVYLVTGTKQNVVLLEQIKALAIYRKLSEKEKEELDDALIEARQAVEMTN
                     LASQILDQLSGTYNNLLNNNLNDTMKFLTVWSLILTVPTIVTGFFGMNLQLPFTHSVF
                     GWGIALIISLVLSIWMLIALWRRIR"
     gene            complement(20777..21436)
                     /locus_tag="ACG842_000628"
     CDS             complement(20777..21436)
                     /locus_tag="ACG842_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HFQ0247190.1"
                     /translation="MSVIVLAGTIGAGKSSLTEMMAEHFDSQAFYESIDDNEVLPLFY
                     ANPEQYAFLLQIYFLNKRFASIKQAMKDDNNVLDRSIYEDSLLFHLNADLGRATETEV
                     RVYDELLENMMEELPYAAHKKHPDLLVHIRVSFDTMLERIEKRGRSYEQLSFDPSLYD
                     YYKELNRRYDQWYEEYKESPKIQIDGDRYNFVEDPQAKEEVLKMIEEKLAEIRQTKVA
                     I"
     gene            21752..22696
                     /gene="mvk"
                     /locus_tag="ACG842_000629"
     CDS             21752..22696
                     /gene="mvk"
                     /locus_tag="ACG842_000629"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004496 - mevalonate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016126 - sterol biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HFQ0247191.1"
                     /translation="MANYGQGESSGKIILMGEHAVVYGEPAIAFPFYATKVTAFLEEL
                     DAMDDQLVSSYYSGNLAEAPHALKNIKKLFIHLKKQHDIQKNLQLTIESTIPAERGMG
                     SSAAVATAVTRAFYDYLAFPLSREILLENVQLSEKIAHGNPSGIDAAATSSLQPIYFT
                     KGHPFDYFSLNIDAFLIVADTGIKGQTREAVKDVAHLFETQPHETGQMIQKLGYLTKQ
                     AKQAIIENSPETLAQTMDESQSLLEKLTISNDFLNLLIQTAKDTGALGAKLTGGGRGG
                     CMIALAQTKTKAQEISQALEDAGAAETWIQGLGVHTYV"
     gene            22689..23666
                     /gene="mvaD"
                     /locus_tag="ACG842_000630"
     CDS             22689..23666
                     /gene="mvaD"
                     /locus_tag="ACG842_000630"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0004163 - diphosphomevalonate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HFQ0247192.1"
                     /translation="MFKGKARAYTNIALIKYWGKKNEELILPMNNSLSLTLDAFYTET
                     EVIFSDSYMVDEFYLDGTLQDEKATKKVSQFLDLFRKEAGLSLKASVISQNFVPTAAG
                     LASSASGLAALAGACNTALKLGLDDLSLSRFARRGSGSACRSIFGGFVEWEKGHDDLS
                     SYAKPVPSDSFEDDLAMVFVLINDQKKEVSSRNGMRRTVETSSFYQGWLDSVEGDLYQ
                     LKQAIKTKDFQLLGETMERNGLKMHGTTLAAQPPFTYWSPDSLKAMDAVRQLRKQGIP
                     CYFTMDAGPNVKVLVENSHLSEVQETFTKLFSKEQVITAHAGPGIAIIE"
     gene            23679..24764
                     /locus_tag="ACG842_000631"
     CDS             23679..24764
                     /locus_tag="ACG842_000631"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004631 - phosphomevalonate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0016126 - sterol biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HFQ0247193.1"
                     /translation="MIEVSAPGKLYIAGEYAVVETGHPAVIAAVDQFVTVTVESARKV
                     GSIQSAQYSGMPVRWTRRNGELVLDIRENPFHYILAAIRLTEKYAQEKNILLSFYDLK
                     VTSELDSSNGRKYGLGSSGAVTVATVKALNVFYALNLSQLEIFKIAALANLAVQDNGS
                     CGDIAASCYGGWIAFSTFDHPWLQEQETQHSISELLALDWPGLSIEPLIAPEDLRLLI
                     GWTGSPASTSDLVDQVHRSREDKMVAYQLFLKNSTECVNEMIKGFKENNVTLIQQMIR
                     KNRQLLHDLSAITGVVIETPALNKLCNLAEQYEGAAKSSGAGGGDCGIVIVDQKSGIL
                     PLMSAWEKAEITPLPLHVYSDQRKENR"
     gene            24761..25825
                     /gene="fni"
                     /locus_tag="ACG842_000632"
     CDS             24761..25825
                     /gene="fni"
                     /locus_tag="ACG842_000632"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004452 - isopentenyl-diphosphate
                     delta-isomerase activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HFQ0247194.1"
                     /translation="MNRKDEHVSLAKAFHDKQKNEFDFVRIIHNPLPQIAVSDVDLST
                     QAVGFTLSSPFYINAMTGGSEKTKKINQDLAIVAREADLMIATGSVSAALKVPSLADT
                     YTIMRQEYPHGKIIANIGAGTSVERAKEAIRLFHADALQIHLNAPQELVMPEGDRDFT
                     NWKVLIQETQTAIDVPLIVKEVGFGMTRETLNDLAALGVHTVDISGRSGTSFTQIENA
                     RRSKRELSYLADWGQSTVSSLLEANEADTSMEILASGGIRNAYDIFKALCLGANAVGT
                     SGTVLTHLMNHGVEETIILMKQWQEELRLLYTMVGATNTAALHQQSLIFSGPVKDWCE
                     ARGIDLVKYGRRTEKSVGQK"
     gene            complement(26074..26877)
                     /locus_tag="ACG842_000633"
     CDS             complement(26074..26877)
                     /locus_tag="ACG842_000633"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HFQ0247195.1"
                     /translation="MEFSMDMFRLDGKVALVTGAVYGIGFEIARSLATAGAKIVFNNL
                     NQESVDEGIAHYKEAGIDAKGYVCDVTDEEAVQAMVQQIKEDVGSVDILVNNAGIIKR
                     TPMIEMDAADFRQVIDVDLNAPFIVSKAVIPDMIEKGGGKIINICSMMSELGRETVSA
                     YAAAKGGLKMLTKNIASEYGQYNIQCNGIGPGYIATPQTAPLRETQENGERHPFDQFI
                     VGRTPAARWGEPIDLAGPSVFLASSASDFVNGHVLYVDGGILAYIGKQP"
     gene            complement(26897..27739)
                     /gene="kduI"
                     /locus_tag="ACG842_000634"
     CDS             complement(26897..27739)
                     /gene="kduI"
                     /locus_tag="ACG842_000634"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008697 -
                     4-deoxy-L-threo-5-hexosulose-uronate ketol-isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0045490 - pectin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HFQ0247196.1"
                     /translation="MQEMETRYTHSPEDIRHYSTEQLRKEFLVEKVFEPGKISLTYTH
                     NDRMIFGGVTPTEKELEITLDKELGVDYFLERRELGVINIGGPGFIEIDGQKEEMKKQ
                     DGYYIGKETRHVVFSSQDAENPAKFYISSVPAHHKYPNVKISIDQVKPMETGEGLTLN
                     ERKIYQYIHPNVCESCQLQMGYTILEPGSAWNTMPCHTHERRMEAYVYFDYANEDTRV
                     FHMMGKPDETKHLVVDNEQAIISPSWSIHSGVGTSNYSFIWAMCGENITYTDMDMVQM
                     DQLK"
     gene            complement(27755..28762)
                     /locus_tag="ACG842_000635"
     CDS             complement(27755..28762)
                     /locus_tag="ACG842_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HFQ0247197.1"
                     /translation="MGKVVTLGEIMLRLSTEQGTRIDSSERFAAHYGGGEANVAISLA
                     NYGHEAIFATKVPDNALGKAVKKHLQCYGVDTKQVLSGGSRLGTYYMETGSGERAASV
                     IYDRAGSSFAEMDHLEWSLEELFQGIDIFHVSGITPALSKKWQKMTKQVIEAAKKAGC
                     LISFDINYRGKLWTQQEASEVIHQLLPLVDICSAGKMDALYLLGIEKAPEAEEQPLIY
                     YYQKMQEKFPNIQVFYSTKRTVHSASENELKGTLWMNHEYVESQVHRITPIVDRVGGG
                     DAFAGGVLHGILSNMSPQEIIDFATAASALKHTIHGDCNQFSQEEVAQFLACGSGKII
                     R"
     gene            complement(28769..29413)
                     /locus_tag="ACG842_000636"
     CDS             complement(28769..29413)
                     /locus_tag="ACG842_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HFQ0247198.1"
                     /translation="MKKIDILSRLKNAGVIAVVRGKSKEEALNACHAIIKGGLTGIEL
                     TFTVPQADQVIKELLSFYKDQPEIVIGAGTVLDAVTARLAILAGAEYIVSPSFDQETA
                     EMCNLYQIPYLPGCMTITEIKTALKSGVDIVKLFPGSAYGPSIISAFKAPMPQVNIMP
                     TGGVSLDNMKEWFDAGVVTVGVGGNLLAPAATGDFDKVTEVAQQYAAKMKEIKR"
     gene            complement(29513..30271)
                     /locus_tag="ACG842_000637"
     CDS             complement(29513..30271)
                     /locus_tag="ACG842_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HFQ0247199.1"
                     /translation="MENKKPYGTVLIKASKILDYLAENQDVSLQTIAKGVGMTSSTVL
                     KILDTLLMIGYVNKNSEKNYRLGAKLIRYANKNIEQIDLVELTLPFLEQLQQKIDETI
                     HLGVLDNNEILYVNKLDPKHQTIRMSSKIGITRPLYNSAMGKAVLAEFSEEQYTDYLE
                     KHPLIPYTEYTITNALRLKKEIKTVKETHIAFDDEEIERDIFCIGASIVKNEQIIGAF
                     SVSMPKYRLTDETKEQIISALKQTKSEIEQRIQK"
     gene            complement(30398..31549)
                     /gene="fucO"
                     /locus_tag="ACG842_000638"
     CDS             complement(30398..31549)
                     /gene="fucO"
                     /locus_tag="ACG842_000638"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008912 - lactaldehyde reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HFQ0247200.1"
                     /translation="MSNRMILNETSYFGKGAIESIVPEFQQRGFGKAAVITDKGLIEH
                     GIATKVTELLDKAAIAYALYDGIVPNPTIQNVKDGVAFVKEAEADCLIAIGGGSPIDT
                     AKAIGIILTNPEFSDVISLEGVADTKNPCLPILAIPTTSGTAAEVTINYVITDEVNHR
                     KFVCVDPHDIPIVAFIDSDMMMGMPKKLAASTGMDAMTHAIEGFITKGAWEMTDMLHL
                     KAIEIIGHSLEASVDGDQNGREKMALGQYIAGMGFSNVGLGLVHGMAHPLSAWYNIPH
                     GVACAALLPTVMKYNKEYTGEKYREIALVLGIKGAAEMSLEDVREAACGEIDRLSKAV
                     GIPETISELGVKEADIPAIAEDALRDVCTPGNPRETTVEEIIALYQSLM"
     gene            complement(31631..31942)
                     /gene="rhaM"
                     /locus_tag="ACG842_000639"
     CDS             complement(31631..31942)
                     /gene="rhaM"
                     /locus_tag="ACG842_000639"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0062192 - L-rhamnose mutarotase activity
                     [Evidence IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HFQ0247201.1"
                     /translation="MVKKAFCMKVYPEKQEEYKKRHDELWPEMRTALREHGVIDYSIY
                     LDPSTSLLFGYLVIEDEKLWEKMANTSINQKWWKYMEDIMETNPDASPVSWELMPVFE
                     L"
     gene            complement(31944..32789)
                     /gene="rhaD"
                     /locus_tag="ACG842_000640"
     CDS             complement(31944..32789)
                     /gene="rhaD"
                     /locus_tag="ACG842_000640"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008994 - rhamnulose-1-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0019301 - rhamnose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HFQ0247202.1"
                     /translation="MKKIDILQAPYVKEMIETTSNLYRLGWDERNGGNISYLLKEEEI
                     TPFLDPEKVIRRISMIFDASRLAGCYFIVTGSGRYFKNVASEPAENLGIVRVAEDGKT
                     LELLWGLENECLPTSELPSHFMSHIARLEVDPDNRIVMHCHASHLLAMSFTHELDERS
                     FSRTLWQMCTECLVVFPEGISIIPWMVPGTNEIGEATAEKMKETRLVLWPQHGIYGSG
                     KDMDEVFGLIETAEKAAEVYTYVKAQGPILQTITDENLWHLADAFGVTPKAGYLEEVH
                     TKAGV"
     gene            complement(32803..34059)
                     /gene="rhaA"
                     /locus_tag="ACG842_000641"
     CDS             complement(32803..34059)
                     /gene="rhaA"
                     /locus_tag="ACG842_000641"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008740 - L-rhamnose isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HFQ0247203.1"
                     /translation="MNTIERNYEQAKEKYATIGVDTDAVLEKIQDIKISMHCWQGDDV
                     KGFLTPDGELTGGIMATGNFPGAARTPEELRQDLEKAYSLIPGKHKLNLHAIYLDTEE
                     SVDLNEIEPKHFEKWVEWAKKEEIGLDFNPTFFSHPMMKDGFTLAHPDKEVREYWIEH
                     GKRSRKIAEYFGKETGQTSINNFWVPDGMKDNPIDRFTPRERLMESLDEIFTEELDER
                     YVQDAVESKLFGLGAEAYTVGSHEFYMGYGLTRDKLVCLDAGHFHPTEVISNKLSSLA
                     LFSKGIMLHVSRPVRWDSDHVVLMDDELQEIAKELVRNELLEKTNIGLDFFDATINRI
                     AAWVIGTRNTQKALLKAMLEPVERLKEMELAFDFTSRMAYTEELKDFPYADVWNYFCY
                     KNQVPVGLDWLEEVQEYQKDVLELRK"
     gene            complement(34056..35516)
                     /gene="rhaB"
                     /locus_tag="ACG842_000642"
     CDS             complement(34056..35516)
                     /gene="rhaB"
                     /locus_tag="ACG842_000642"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008993 - rhamnulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0019301 - rhamnose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HFQ0247204.1"
                     /translation="MNPTFVAIDIGASSGRLMKSTRDAKGSLILEEVHRFKNGFRNKD
                     GHDRWKIDYLIKEILIGFEKLKQTGIDRLFAGIDTWAVDYCLIDEQGDRINDPIAYRD
                     KRTNGAVEQFSEIYSLDKLYEQTGIQIQPFNTLFQLFAEEKENLAKAHKLLLIPDYLG
                     YVFTGNMVTEKTNASTMQLLNAGTKEWEIELLEKIGVSKELFPPLTEPGTILGDLQKE
                     QFSKYDLPDVTFITVASHDTASAVLGTPGQGERWGYISSGTWSLLGIETKIANVSRNA
                     FSENYTNEWGAHNTVRFLKNIMGMWLIQEVARMQEYEYSYAELAELAAKEKPLQQVME
                     INDPRFLNPPNMIEEIQQYCFETEQPVPVSSGAIARCVYDNLALCYAQELIKVEHLTG
                     TKLEKLHIVGGGSNNRLLNQLTADYAGIIVEAGPSEATAIGNVAMQMLSTGQFSNIEQ
                     ARNVIKQSFPCETFYPDENKQNTMEWYVNKIKGVIV"
     gene            35669..36538
                     /locus_tag="ACG842_000643"
     CDS             35669..36538
                     /locus_tag="ACG842_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFQ0247205.1"
                     /translation="MDYWEKANHSWSEDSKRVILTPTQRSRNLFFYIQEIGYFKAPIP
                     YYTERAHLPSYLIKFTLSGKGSLTYNDNQQQLKAGDLFFIDCQEYQRYQTISEEPWEM
                     DWIHLNGQNVQSFYQEFTKDGHNTFHSTGDPSENKIHHLITELIHMQQGTNARTDFQS
                     SVAIHELLNELILQKYQLDFEEEDIPAYVYKLKQYLDEHFKETITLDQLEQQFLLNKY
                     QLSKDFSKFIGSPPIDYLISKKLSYAKDLLRYTEFTVRMISEEIGIENFAYFSRLFKA
                     KTGLSPRDFRKFS"
     gene            complement(36664..37563)
                     /locus_tag="ACG842_000644"
     CDS             complement(36664..37563)
                     /locus_tag="ACG842_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0247206.1"
                     /translation="MLQKKISGEQVNTMVSDQYEIYHVKDVVSQNKTIYHHHDFYEIH
                     ATLKGEATFYLNGRQFTIKAGSVLLIHSNDLHRIVRQSSDVFERVYIFVTPSFLESRS
                     TPWSNLSACFQPMGEKRSRVLQIEQEVLKQKLSFIDHPPDKKIYGEDIKYEQALVEYL
                     IFLNQVVQKEESAQEQNAVVPNERVEQMLQYISKNLSHPLSLEEMEKQFFVSKYHITR
                     EFKKYTGFTFHQYVMKKKLIYAKHLLREYRSSSAVYSKSGFSSYPHFLKAFKREFGIT
                     PKEFLKRDTQKQLVHYEHFEDEQ"
     gene            complement(37728..38552)
                     /locus_tag="ACG842_000645"
     CDS             complement(37728..38552)
                     /locus_tag="ACG842_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HFQ0247207.1"
                     /translation="MLNITIRGHDLSEVQTIEDLAEKTKEQGIHTLQLALGLSFPEIP
                     SGANEINSGMGNYVKRVLEKQEVTVGILSCYINMIHPDLTIRQELLTKFEQYLRYASS
                     FGASMVASETGCVLPEIQYTEENFTDEAFAEAVSVIRRLVKAGEKYQMMVGIEPGLNH
                     PVYSLARVEQLIQAVDSDYLGIILDPTNLITSSDYQEQVQLVEEAFERFGEKICAVHL
                     KDFRVEQEKIVPVNLGDGVIEYTKIKEIIMKNRPYLYVVLEETKDDGIRYGRSLLE"
     gene            complement(38563..39858)
                     /locus_tag="ACG842_000646"
     CDS             complement(38563..39858)
                     /locus_tag="ACG842_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HFQ0247208.1"
                     /translation="MAIEAGLVLFFVFVVLLVLGMPIAISIAFSSMMTLLLVIPFDVS
                     AFSSAQKMVGSINSFSLIAIPFFVLSGIIMNNGGIAKKLVDFAMLFVGRVPGALAHTN
                     VLGNALFGSISSSAIAASTAIGGVLIPQQVEEGYDRKFATAVNIASAPTGMVIPPSTA
                     FIMYSLVAGGASISALFMGGYLVGALWALGIMLVAFIVAKKNKYPVVEKGQMAHAGKI
                     IKEAIPSLLLIVIIIGGILTGIFTAVEASAIAVIYSLLIAMFYYKTVKLKDIPNMLFQ
                     SVAMSGTIMFLLATSSMMSFAMAFTGIPQAISSLIMGVTDNKFIILLLVNLVLLLVGM
                     FMDVAPAILIFTPIFLPIVTSVGVDPVHYGLFSIMNLCVGSITPPVGTGLYVGASVGG
                     VKAEAMLKPLLPFYGVILVVLFLITYFPQIVMWLPNMLS"
     gene            complement(39874..40353)
                     /locus_tag="ACG842_000647"
     CDS             complement(39874..40353)
                     /locus_tag="ACG842_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HFQ0247209.1"
                     /translation="MKMILNKVLEILGAVLLVGMVAVVLWQVFSRTVLGNPNTVTEEL
                     VRFGLVWFAMLSSAYVVGQKAHLAVTVLSEKLTGKKSKILEVVVQSLFLVFAAMIMVY
                     GGWNAVTLTMGQISPSLSIPMGYVYLSVPVSGVLIIIYSLINLIDTLSNKPAIQEKI"
     gene            complement(40359..41387)
                     /locus_tag="ACG842_000648"
     CDS             complement(40359..41387)
                     /locus_tag="ACG842_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HFQ0247210.1"
                     /translation="MKKTSLLFAASSLSLGLLGLSGCGNNSSVATVADADVNEIVTLR
                     FAYASNSQPVIDAMNEFGRLVKEKTDGSVQIQYFPDGQLGGERELIELTQSGAVDFTK
                     VSASALESFSKDYSIFSVPYLFNDEEHFFKVMDNEEIMEPIYQSTKNLGFTGLTYYDS
                     GQRSFYMVDGPIHTPEDLKGKKIRVMQSETAIKMVELLGGSPVPMGSDEVYTSLQSNL
                     INGSENNEFVLYTAGHGGVAKYYSYDEHTRVPDIIIMNDAIKEQLTDEQEKAIEEAAK
                     ESTVFEIEAFAKAIEEEKALAQEEHGVQFNDVDNTPFREAVAPLHDIFKNDPTYKDLY
                     QKIQEQGA"
     gene            complement(41404..42510)
                     /locus_tag="ACG842_000649"
     CDS             complement(41404..42510)
                     /locus_tag="ACG842_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HFQ0247211.1"
                     /translation="MIIKEEIFVPKELVKEKIDLLVENLTTIKDDNGEFLLDFDGLKV
                     DDKSWTVWNWPQGVGLYGIYKNYQMTKNPRAYQVVNEWFEDRMEEGAPPKNVNTMAPL
                     LTMAYLYEDTKDSRYLPYLEQWAEWVMNDMPRTNEDGLQHATYGPENKNQLWDDTLMM
                     TVLPLAKIGKLLNRPEYLEEARYQFMIHIKYLMDKRTGLWYHGWTFEGNHNYAEAFWA
                     RGNCWLTIAIPEIIEILELSKEDALRKLLIETLEAQVRALKTYQSESGLWHTLLDDPS
                     SYVESSATAGFAYGILKAVHKRYLPQEYKEVAYRAIQGLLEQIDEKGEVQNVSIGTGM
                     GDSLDFYRNIGITAMPYGQSLTVLCLTELLVSYC"
     gene            complement(42739..45336)
                     /gene="adhE"
                     /locus_tag="ACG842_000650"
                     /gene_synonym="adhC"
     CDS             complement(42739..45336)
                     /gene="adhE"
                     /locus_tag="ACG842_000650"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004022 - alcohol dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006066 - alcohol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HFQ0247212.1"
                     /translation="MAKVIEKEKTKTIDVQAMIDDLAEKANAALKEMESFDQEKVDHI
                     VHEMAMAALDQHMPLAKMAVEETGRGVYEDKAIKNMYASEYIWHNIKHDKTVGVINED
                     VQKGLIEIAEPVGVVCGVTPTTNPTSTTIFKSMIALKTRNPIVFAFHPSAQKSSAEAA
                     RVVRDAAIAAGAPENCIQWIEHPSIEATSMLMNHPGIAIVLATGGAGMVKSAYSTGKP
                     ALGVGPGNVPAYIEKTAKIKRAVNDLIVSKTFDNGMICASEQAVIVDKEIYAAVKAEF
                     QAHQVYIVKPDELQKLEDAVMNEGKYAVNPSIVGHSAMEIAKLAGISVPKGTKMLIAE
                     LEGVGPDYPLSREKLSPVLAMIKANNTDHAFDLCEGMLNLGGLGHTAVIHSENEELHV
                     KFGLRMKACRILVNTPSAEGGIGDIYNEMIPSLTLGCGSYGKNSVSRNVSAVNLINVK
                     TVAKRRNNMQWFKLPPKIFFEKNSLLYLEKMENVERVMIVCDPGMVQFGYCDTVREVL
                     ARRKNDVKIEVFSDVEPNPSTNTVYAGTKLMADFKPDTVIALGGGSAMDAAKGMWMFY
                     EHPDTSFFGAKQKFLDIRKRTYKIDKPEKTQFVCIPTTSGTGSEVTPFAVITDSETHV
                     KYPLADYALTPDVAIVDPQFVMSVPASVTADTGMDVLTHAIESYVSVMASDYTRGLSL
                     QAIKLVFDYLEKSVKTPDMESREKMHNASTMAGMAFANAFLGICHSVAHKIGGEYGIP
                     HGRTNAILLPHIIRYNAKDPQKHAMFPKYDYFRADTDYADIAKFLGLKGETTEELVEA
                     LATAVYELGKSVGINMSLKAQGVTQETLDTTVDRMAELAYEDQCTTANPKEPLISELK
                     QIIIDAYNG"
BASE COUNT        13161 a         9672 c         8254 g        14397 t
ORIGIN      
        1 gtttattact tactttttct tattctctag taagctaata ttgaaaaaaa tgtaatcaga
       61 tcattatttt tatatgttgt aacttcctga taagaaaact aattaaaaga aagaggtaac
      121 tacataaact aattaaaaaa gctaacttca tagttggagg ataaatggaa gaaacttcaa
      181 tgattgtttc tccttgtgtt ccttcaatcg acactagtcc tattatcaat ctctcttttt
      241 tgtccattta cttttgcgac tgttccccaa taatcaaata caggaagcgt tattttttgt
      301 cctttagatt catgtactgt atagactatt ttcgaatcca tcacttgata aggcatagta
      361 tgccattcct tgccaattct tacttcatga ttctgcaaat gttctttgta ttgtaatgct
      421 tccgtgggtg catagtcata aagtcctccg ggaaggagtg tcgtgtaacg aataagattt
      481 tcttgattca ttaccaagtg atctctctga gtgacaactt tatgttcata gtttgctttt
      541 gttgcagcag atacagccaa cagtaataaa acaattgccg ttatattaac tacttttctt
      601 tgcttagctg atgtgctctt catgaaaacg aacgataaaa tcaacgagcc aaacagtact
      661 tgaaagccta agattcgcca aggaaattga atcatttgaa tgggtgtatt ttgtaataaa
      721 gaccatggaa aaaatttggt ggataagagc caagagacta ccgcactaag gtaaatcact
      781 ttttctttgc gattaaatcc tttaaaaaag aagagtggac agatcaaaga aataatacca
      841 atgattccta ccgtaaaggt tgtcaggtca ttattcatcg caccaatcaa actatccgag
      901 acatttaatg cttggtgttg taaggtcgtt ttatctggct cattaatcga ttgaaccatg
      961 atttgtcgga acatcggata ccaaaagtat gctgttaaaa gaatcgtcat tcctactgcc
     1021 tttgacaact ccacaatcaa ccggatgctt tctttttttc ttgtttcact tactacaaag
     1081 ccgcctaaga ctagtaagcc aacggctatt acactcataa aagtggaaag aacatgtgta
     1141 taaatgagta agctcatact gactgctaat ggaattgcac atggcttttt ctcataaatc
     1201 agtgcgtaag cataatacat aactatcggt ataaccgtaa gtgcaatcgc ttcagcaata
     1261 gcagaacgat gataaatatc aatcgtacgg tacccataaa atgtatataa acagctaaat
     1321 ataaaagccg catcgattct ttttaaaaat tttagtccat aatgaaacat cagcatgata
     1381 gtacaaacat tcaatagcca aacatataag atataactaa cgattaaatt attacttatc
     1441 caataaaaaa tgactgccgg aaaaaaggtc aagtagggat aaaaataatt cactccgctc
     1501 ccataatgat taaacgtcgt aaaattgatt ggaccagaaa aaattgaaga taaccctttg
     1561 actctagcaa aatggaaggc aatatccccc gatgtagaaa tagtatgaac atttctaaaa
     1621 taattgattg aataaagtcc agcaataatt gctaggagaa tggacaatat tatttttttc
     1681 tttacttgca ttacttaatt atatactcct ttcatggatt gctttatcta gtataactta
     1741 aattttaaat aaatcgttat ttatttttac tctcacttaa aaaagcacta aaattataag
     1801 agagggaggt aatggtctga acaccaagaa ttgttccaac atcgaattgc ttaactaatg
     1861 agttcaaagc ttaattatgt ggctcttata tttaccgagc gtggattgcg atctctttag
     1921 cgaaacaaac atctttcata aaaaaaacag ccatatccgc tttcttggat atgactattt
     1981 actactactt tactatcttt cattcttctt gtctcactta tgataaggcg cccccgcatt
     2041 gatccggaag gcacggtaga tttgctcgac aaggatcaga cgcatcaatt gatgtggata
     2101 cgtcattttt ccaaaagaga tctgagcatt gcttcttttc atgacggctt cgctgagtcc
     2161 taaagagccg ccgataacaa aaacaaggtg gctttttcct tggataccta attggtcgat
     2221 ttgtttagca aaagattcac ttgtaggatt tttcccttca attgctaagg catagacgta
     2281 ttcttgttct ttgattttcg ctaagatccg ttccccttct ttttctttta cttgtctcat
     2341 ttgcgcttcg cttaaattct ccggggcttt ttcgtcagga acttcgatca attcgatttt
     2401 tgcataggca ttcagccgtt tgacatactc attgatacct tggatcaggt atttttcttt
     2461 taattttcct actgagataa ttttgatatt catgttttct ctcctttggt cttcatgctc
     2521 actttgttta ttataatcaa ttttttcgtc tatatgttta ttttggctat ttttgttaaa
     2581 aagatttccc caacagttat gcacaacatc cacaggttta tccacagaaa acagtgttat
     2641 tttaccttat ttcatcacat tcattaggca aacatacgtt ttataagaat ttatccactt
     2701 tatacacagg ctgtggatat gttttatccc ttatttttcc aatcctatcc gcgtagtttt
     2761 acacagtatc tgtggaaaaa gaaaatcaaa tattttcatc tgaaaacaat gatttttcct
     2821 aaagtttttc ataaaaagtt cacagtctca aagtagacta aagtcaacaa ataagactat
     2881 actaacagta gataaatgaa tgtaattgga ggaggaacac atggatagaa aaaatgtgac
     2941 accaaagatg aaaaaaagta aaaataatag cctctggcgt aagttaggtc tggggcttgt
     3001 tggagggatc gttggaggac tactgacagc tggcattttt tatgcagcga tgggcactgg
     3061 caacaatagt tctactgcta caagcggcaa tcagaattct gccggtgaaa cagttgtgga
     3121 aaatgtcaaa gtcaatgtag attctgatat caccaaagcg gtagataaag ttcaagacgc
     3181 tgtcgtttct gttatcaact tacaaagtca aaaccaatca agcggttttg gcggactatt
     3241 cgggcagcaa gaagaaagta gcagctcaga tgatagtaat ttggaagctt atagtgaagg
     3301 aagcggcgtg atctacaaaa aagacgggaa tactgcttat gtagtcacga ataatcacgt
     3361 agtagatggc cagcaaggtc ttgaagtctt gatgaaagac ggtacgaagg taaaagctga
     3421 attagttgga acagatgctt attcagattt agcggtgttg aagatcaatt cagacaaagt
     3481 cgaaacagtt gcctcttttg gtgactcaag tgctctgaag gttggtgagc ctgcgattgc
     3541 gattggttcc ccactaggat ctgaatatgc aaattctgtc acttcaggaa tcatttcttc
     3601 cttgaaccga caagtgacaa gtacgaatga atcgaacgaa acagtcaata tcaatgcgat
     3661 ccaaacagat gcagcaatca accctggaaa ttctggtggt cctttagtta atatcgaagg
     3721 acaagttatc ggtatcaact caagtaagat cgcaagtact tctgaatcta cttctaatgt
     3781 aagcgtagaa ggaatgggat ttgctattcc aagtaacgat gtagtgaata tcatcaatca
     3841 actggaaaaa gacggaaaag tgacacgtcc agctctaggt atcacaatgg tcgacttatc
     3901 tgccgtttct actcagcaac aagaacaaat cttgaagatt ccttcatctg taactaacgg
     3961 tgtcatcgtg acaaatgtcc aaacagctac accggctgaa aaagctggat tgaaacaata
     4021 cgacgtcatt actaagatcg atgacaaaga agtttcttct ggtgtagaac ttcaatctgc
     4081 tttgtataag aaaaaagtgg gagattctgt caaaatcact tactatgaag gctcgaaaga
     4141 aaaaacaact actgttgaat tgacgatcga tcaatcggca ttaaaacaaa atcaaaatag
     4201 tgaaaattaa taaaaaagag gttgtgacaa agacttgtca caacctcttt attttggaaa
     4261 tgcttctccg actacggcat cctcaggcaa tactaagatt cctttttttg ctggagcatt
     4321 tggcaaatgc aattcacgag cggaacagat cattccatag ctatctaccc ctcgcaaagt
     4381 tcctggccag atcatcattc catctggcat catagctcct ggttttgcaa cgacaacttt
     4441 ttgtccagct tcgatattgg gtgcaccgca aacgatttgc aatactgtac cattgtctac
     4501 ttctgtctca gtaatcgata aatggtcaga atcaggatgt gggacgcatg tttttacgta
     4561 cccgacaacg atcttcggtt catgatcagc ttcaagctgt tcttcgaatc ctgcttcttt
     4621 gatcaatcga ttcaatttcg ctaattcctc atctgttaag cttacttggc cgttcccttc
     4681 gattgttaga tgatcggaga tatggaaaaa gttccaagca acaacttgac cttgttcatc
     4741 tttgatccga gcaacattcc atttgcgttc aacagtgttt tctgttcctt tatcgtcggc
     4801 tataataacc atcaaggtgt ctccgacgtg tgctttgtta taagcaaaaa tcatgttctt
     4861 tcctccatta tcctttgctg agatttatct taacaaaaaa ttgcattcac tgcatgaaat
     4921 ttttttcatg ctttatttcc gaagaagcct cttctggaac accatttatt cgtaacaagg
     4981 gactgtgaca agacttttgt cacagtccct ttactgtttt agttaattga ttaaaaaggt
     5041 cgtacaagtt ccacttgctt aattacttgg ctgttcttct tcgtcagaaa cgatcaagac
     5101 atcacaaggt gaatgacgta tcacgtagct ggctacactt cctgtcataa agcgttcgac
     5161 tgcatttaga cctgactgcc caaccatgat tagatcgatc tcatattttt ttggcaattt
     5221 gactgccatg gattcacgaa cagaaccata tgcaataatt ccttctatat ttgtgtaatc
     5281 aacagatttc ccatattctt tacattcttc gatcatttcc ttggctgttt cagtttgctg
     5341 atcgactaga ttttgatcaa taggtgcata gcccataaag ttataaaatt gctgatcgat
     5401 tacgtttgct acatatactt tcccattatt acggcgagcc acttctaccg cttttttgaa
     5461 agcagcaagt gcttgttcac taccgtcgat acctactaaa atattcttat attcttgatt
     5521 catcttgacc cctcctaaca gatgtcagcc ttttgttaca ctttctagga acgattcgat
     5581 ttcctgtttt gttttacgat ctttattcac tagacgacct aattcttttc cgtcttggat
     5641 cacgacaaaa cttgggatac caaaaatgtt ccattcagcg gctacatcta tgtacttgtc
     5701 acggtctact tcaataaatt gccaatcaga aaagtcattt tcaatttcag gcatttgcgg
     5761 tttgataaaa cggcagtctc cgcaccagcc ggcagtaaag aaaaatacac tctttccagt
     5821 ttctacgtag cctgctaatt cttctaaact atttgggata atcattcttt gtcctctact
     5881 ttctgtttat gtatttttca attaaattat acacctttct ttcgaactaa caaacattga
     5941 tcctcttttt ataaaaaaaa tctggcaaaa agcaatcttt tgccagattt tatagtttca
     6001 ctatcgcatt attttccgta tacgatcgta tttactgttg atttatccaa acctttcagt
     6061 gtttgaatca acatttctcg tgcagcttca aaatcgcgaa tactgaacat cgtttgatgc
     6121 gtatggatat aacgtcccac tacgccaatc actgtacttg ggattccttc gttttgtgta
     6181 tgagcagcac ctgcatctgt accgcctttt gaaacaaagt attgataagg gatattgttt
     6241 gtttcagcga tatccagcaa gtactcgcgc aaacgaggaa gcatgatcaa acctggatct
     6301 tggatacgca tcaatgtacc ttctcctaaa tgtccgaatg tgccattttt cgtcactgta
     6361 tcatctgctg cagagcagtc tactgcaaag aacagatctg gcttgaattt attgacagaa
     6421 gctttagaac cacgcaagcc tacttcttcc tggacattcg cgcctgcaat caatgtatga
     6481 cctaatgttt cattttgcaa cgcttctaaa gcttctaata ccattgtaca gccatagcgg
     6541 ttgtcccatg atttactgat aatattcttt ccgttggctg ttttgatcgt ttcagcgtaa
     6601 ggaacgattg tgtctcctgg aaggacaccg taactcattg cctcttctct tgattcaaac
     6661 ccagcatcga acaaaatatc ggttacttct actgattttt gtccgcttgt tcctcgcaat
     6721 aaatgtgggg gaacggaaga agaaatacat ggataattct taccagtaga ggttttaagt
     6781 gtaaaacgct gagcagaaac aacgtaagga ttccagccgc ctagaggtac gactttaaaa
     6841 agcccgtttt cttggatttg tgtcagcatg aatccgactt catccatatg tgctgcgacc
     6901 attacacggg gtgcatccgc ttcttcatga tggcgcagac caaaaatgcc gcccaaacca
     6961 tctaactgta actcatctac cagcggttcc atatgacttt ccatataagc gcgaacatct
     7021 tgttcaaaac cgcttgttcc ttgtaattct gttaactctt tgattcgttg aaatgttttt
     7081 tcttccatat gctattcgtc taaatttcag actaaactcc tctcagactt atattgatct
     7141 gcggataaaa tatgattttt tctgcagacc gatcgtattt attatagctt attttttctg
     7201 gtagcggtta tttatttcaa agaaaatatg agagtgtggt aaaataagca caaggagtaa
     7261 caagtgagaa gaaggaggta tgtccacatg tatgaagaaa gcgaactaag ctattttaaa
     7321 ggcgggttag cagttggaat cggtctagga ttattgagcg gggtagcttc aacgatttgg
     7381 tatcatcgca aaaaagtatt gtcagcagac caagtacttg aaaatatcaa gtcatctttt
     7441 ctaaaagaag gtgcaatcga aggttcttgg atctcttttg aaaaggaacc tattaggaaa
     7501 ttcgccgttc ggtcgagtgc ttatcgggga ggtctttctc gaatagaaga tggcgaaact
     7561 gtttattatg aatttttagc agatgctcat acaggaaccg tattagaaat ttcaagaaaa
     7621 aaaggtgcaa cagattaata cgtcaaaaaa gtgagagaac gaatacgcac ccactattca
     7681 aaagagaggt atcatgtcct tcttttgtag tgggtggttt tttagataga gctttccaaa
     7741 catatattcc agtattttct ttttaaataa atgatattta gcgggaaaaa ctagcatttc
     7801 ctttgattat gaggtatttt taatttagct tttgttttct atagttaagg agagtgaaaa
     7861 atggaacaac aagaaaaaat tgctgttctg attccttgtt acaatgagga agcaacaatt
     7921 tcaacagtca ttgcagactt taaacgagaa ttaccagaag cagatattta tgtctatgat
     7981 aataattcga ccgatcgaac atacgaattg gccgttcaag gtggagcaat cgtaaaaaaa
     8041 gaacctcgtc aaggaaaagg aaatgtgatc cgccaaatgt tctttgatat cgatgccgac
     8101 tattatttaa tggtagatgg cgatgatact tatcctgcag aagctgtaca tggtttgctc
     8161 gagaaattac gttctggtga agcagatatg gtcattggtg atcgcctatc aaatggaacc
     8221 tactttgatg agaataagag acctttccac gattttggaa ataaccttgt tagaaataca
     8281 attactagaa tgtataaaac aaaaattctt gatgtcatga ccggctatcg aggattcaat
     8341 cgtatctttg tcaaaagttt tcctattatg agttctggtt ttcaaatcga aacggaattg
     8401 acgatccatg ctttagataa aaaattcaag ttcgttgaaa tcccaatcga ttatcgtgat
     8461 cgtcctgaag gcagcgaatc aaaattgaat acattctctg acggattcaa agtgatcatg
     8521 atgattatta aaatgtttaa agattataag cctttgcttt tctttgggat ttggaccatc
     8581 ttcttctttt tatttggatt ggtcacgggg attcctgtga ttcgcgaata tatgctgaca
     8641 agctttatta cacgcatccc ttctgcgatt ttaagtactg gattgatgac tttagcactt
     8701 ctttcattag taacagggtt gattttggat acagtcgtca cgaacgctaa gaaagaatat
     8761 gaattgaatc tatatcatgt ttatcatgaa taccacagta gaaagtaaaa aaaggatcaa
     8821 gactgcaaaa ataatttgca attcttgatc ctctttttaa ttaattattt cgccaaacga
     8881 tagatggcat ctgcatagat tgctgttgct ctgaacaagt catcaagact cataaattcg
     8941 tttgcttgat gcattgtatc agtatatcct gggaacatcg ctccataagc aacgccacgt
     9001 ttcaacaaac gtccgtaagt tccgccaccg atgatttgtt cgtagccttt ttctccagtg
     9061 tggtcttcat agacttgcaa taatgtagcc accaatggat catccattgg tacgtaatgt
     9121 ggttccatta cgcgagcacc tcgagtaact gttgcgcctt cttgccccac tgtttgttcg
     9181 atgtcacttt gcaatccgtc tactgttaca ccttttggaa aacggaaatt catattgatg
     9241 aagttcgctt cctctgtacc attttcttca aaagcaaata gtccagcatt cattgtcaat
     9301 ttgcccattt tttcatcttc ataagcgact cctagttttt ctccataaaa atcttcatgg
     9361 acataagcag ctgctacatg gataaattgt ttagcagatc ctaggaattc atagttatct
     9421 aagaacaatg ccaagaaaga acctgcattg ataccagatt gtgggcttgc accatgtgcg
     9481 cctttaccaa ctaattcgat ttttacatac gtattgtccg cttcaatcgt tccgcttact
     9541 ggatttgctt cgatgaattg gtaaaatgct tcttccatcg cgatagctgc atctgcactt
     9601 ggtacttgaa gtgctgcagt tgctgttcct ggcaccatat tttcgcgaag tccggaaaca
     9661 aaagatttca agacataatc tcctgcattg tcgcctttga agcgtaaagc taaagaaaca
     9721 tttccttttt caccattgat gattgggaat tcagcatctg gtgagaaacc aaaatctggt
     9781 tcttcttcat gttcgaaata ataagccata tcgccccagc cgctttcttc atcgctgccg
     9841 actacgaagc gaactttttt tgatagtttc aagtcaagtt ctttaatgat cttcatcgca
     9901 tagtaagctg ccatacttgg acctttgtca tcgcttgatc cgcgtgcaaa gatctttcca
     9961 tccttgatta ctggttcata aggatctgtg tcccagccat ctcctgcagg aacgacatcc
    10021 atgtgtccaa atatccctag cgtttcgtct ccttcgccaa gatcaatatg ccctgcatag
    10081 ttatcgacat tttttacttt aaatccatca cgttcgccat aagctaacat atgtttcaat
    10141 gcgtcgcgag gtccaggtcc aaatggtgca tctggtgtta cttttgagtc atcgcgctcg
    10201 ctgttgacac gtaaaagatt tttcaagtct tcaagcaagt cttctttacg tgcttctact
    10261 tctttttgcc aatcaattgt cataaagtta atcctccact tatttaagtc ttcgtgttca
    10321 tgataccaca aattacagaa aaaaatagtt caaaaaatct gacaattcct tttcttatct
    10381 gtttttctct aaaaaaaata tacgctataa tgagtaaaag gaggttttta gatgctgata
    10441 aacgaacaac tagtgaaaag agtcatcaca cctcgtccaa aaatttcaca taaaggaaca
    10501 tttggacgta tcgttttgat tggcggcaat gaacaatatg gcggagcaat cattatgagt
    10561 gctttagctg ccgtacacag cggagcgggt ctcacgactg tagtaactga tgaaaaaaat
    10621 cggagtgctc ttcacagtca tctgccagaa gcaatgattg tagtttggac ggatactgac
    10681 cagtttcttt ccgtaataaa gcaagcagat gtactcttga ttggtcccgg attgggtact
    10741 tcttctaaga gtttggaacg tttcaaacaa gtactagaaa accaatcaaa agaacaatgg
    10801 ctggtgattg atggctctgc tttaagttta cttgctgcta atcatctttt gctaccctat
    10861 cccgaacaaa ctgtacttac acctcatcaa atggagtggc aacggttgag ttctctaccg
    10921 atacaagaac aacgggataa agaaaacaaa aagaagcaac aggaaatcgg tgctgtgctc
    10981 gttttgaaaa gtcatgagac aaaaatttat ggtcaccagc acttctacac caatccttta
    11041 ggttctgctg ctatggcaac gggcggcatg ggtgacactt tagctgggat gatcacagga
    11101 tttttagccc agtttcctca aaatgaccaa acgattgcag cagctattta tcttcatagt
    11161 ttcattggag accagctggc aaaagaacaa tatattgttt tgccgacaca aatcagcgaa
    11221 gcacttccga aatggatgaa ttatttttgt aaaaaatcaa tggaaaacga ggcatgagtc
    11281 atggagaaat ttacaggtta tgtgcgagaa cctttttatt atgaaacaga tcaaatgggg
    11341 attattcatc acagcaatta tatccgctgg tttgaagagg ctcgggtagc cttattggta
    11401 catctagggt ttccctataa aaaaatcgaa gaatctggga tcatcatccc cgttctggaa
    11461 gtaaattgca gctataaaga aatgatccat tacggagaaa aagtgagcat ccatccaact
    11521 atccaaaagt atacggggac acggttggat tttagctacg aaattgtggg aactgaagac
    11581 aaaaaattaa aaactactgg cagtagtaaa cactgttttt tactttctga ttcgcaacga
    11641 ttagtgaagc tgagcaaagt caatccggag ttagatcagt tattccaagc gtacgcttct
    11701 ttggatgatg taaattctta ataaagaccg tctaagcatt tttcttgtat tgtgtcgagt
    11761 agttcgtata atgaaaagaa aaaagcaggt gatccccatg tatgtcgtta aagtaatgca
    11821 cggttacatc gataaaactg gctgtcgcac tcgggaaaaa aacttagaca atctgctcat
    11881 tttcaaagac aaaaaagaat ccgaagcttt tgctaaaaga atcggcggtc gtgtcaaacc
    11941 gatccaagag gtacgaccag attgattcaa tatttttctt ttcgaggctc taataggcat
    12001 gcctatcagg gctttttttg cagactgtgt ttttatgccc acacgatttg taagcgattt
    12061 atgttataat agattatatc tgcatagaaa ggaaggttaa atggttatca ataatttgca
    12121 acagcaagct tatgaatcta tacgtaaaca aatcatttat tccgagttag cacctggaag
    12181 taaaatctcc gataaagtgt tagaagaaca actagctatc gggcgtacac cgattcaaga
    12241 agcactgatc caactgcgca atcaagagct tgtaaaaacc attccacagt ccggcactta
    12301 tgtttcaaaa atcgatatcc gatctgcttc attagcccgt tatacgcggg aaaagttgga
    12361 aaaaccgatc ctgcaagaat gcagtgcgaa aatgaccaag caagatcaag ctaaactaga
    12421 aaaaatacta gaacaaaccg atcaggcagt aatcgctcaa aataaaaaag ctttttttga
    12481 tttggatact gattttcacc gaacttgtta tgaaattgct ggaaaacgag aaatttggga
    12541 ttggctagaa agttatagta cgcacttgaa ccgtttcaga tggttacgtt tgacgatttc
    12601 agaattagac tggggtcgag tattagatga acatcagaca atgcttcaat caatgattga
    12661 tcataacttt gatgaagtcg gatttctttg cacgatgcat ttacatatga ttatcgagga
    12721 gcaagaatac gtgattcata attaccccga ttattttgaa gatattcagg acagacccat
    12781 caaataagag gttttggaat cagcgttttg acctgagtat tagagaaaga aacggcaaaa
    12841 tagttcctca tattttgccg ttttttttga gctaataccg agggtcagct gattgcacac
    12901 cgtttattga gaggttgtga aaaagctgcc ctgcatcaag taataagaac agccaagtaa
    12961 aaatagcttc ttatgttttt aacttggctg gacttattac cgcagatgca agcttttgaa
    13021 caccgttttc caagaggtcg tgagcctgcc gttcatctcc gagtcacaag gaacaatttc
    13081 aaaaaacagc ttctcatgtt tgttgaatat tttgacttgt gaccgaggag ttgacaggag
    13141 aacatcgttt attcggaaaa aaaggactgt gacacgactt ttgtcacagt ctctttattt
    13201 gtaatctaaa tcatactccg tacatctttt ttctattttt tttatttctt ctaagatccc
    13261 ttggctacga atttcattat atggccaaag taagactgcc tctaatacat aaggcgaaaa
    13321 atcgctttga ttcatctctt ttgaaaaact catcgctgct atctcgctca atgcacgaat
    13381 gtttgattga tgtgtataaa aaggcaaccg ccacagcaca attttttcgc tttgcgtata
    13441 aattccctta acagaagctt cgatgtggtg aaatagttca tgtccaataa tcaaagcttg
    13501 cgctgtttct ttagagatcg aagctagtcc tgaacatttt aatttttcta acggctccgt
    13561 catcaatcga atcccttcat cttctgcata agttgcaaaa gtgattcgtt cgtttatttg
    13621 ctctgattca caggaaacga ttggaacagc catttgttca caaatttctc ctacatcggc
    13681 aaaagggtac ttttttttga tgctacatgc cgtttcgata ccacaatcga ttgattgttg
    13741 tataagaatt ttctgcttgg acaaaggaat ctttttccaa aataaatctc gccgaaactg
    13801 gtagaatccc caaatttctt cggggatctg ttgtaattcg tttagtaact cttctaactt
    13861 atgtttgttt gatatttccg gcaacgacga tcacttcttc ctccggttcc atagtagata
    13921 cttccagatc catcaaaagc tgtagttgtt ccagatccgt cgctgtaaaa tcagagactc
    13981 ttgctcctaa gcagagataa aattctggtc gggcaatcaa ttcagtttga taaactgcca
    14041 tatcttccca aagttgttcg acagctgtca tataattggc tgcagttgtt ttcatacatg
    14101 aagcatgtcc acgttggacc ttgataaatc ctttttgatc gatgatccga agacttccag
    14161 cattttttga acgattctgt tcgccgcata cataaaagaa aggtgtattt tccaataagc
    14221 aaacttcatt tttatttaat cgcatatcct ctgcagcaag ctctagtgct tcttctgttg
    14281 tgatctcctt ggttagatcg gttgctttca cttccgtaga accagtagcg atagccgtta
    14341 cttttgaagt ctgtggatcg atttctacgt ggacttcgat cgattctggt gtagcgcctg
    14401 attcaatcgc tttattcatc gcttcatttt tcaacgatcg tatatcttct ttacttggcg
    14461 aagggatgat acgttctaca acatctcgga ccattgcaag tgctacacca atagaagaaa
    14521 tcacttcggc gttttcagga atcgaatatt tcacacccat tttattacta aagtaagtaa
    14581 tcaatgaggc agcgccgccc cctacaccta caagagaaat ctgatctttc tccaagtgat
    14641 acttgtctgc caattctaaa atcaccggtt caatcttagc ataggatttt tccatgatct
    14701 gttctgcgat gtcttcaacc gtcgtatgac aataatcagc taatgcttgg atggcttttc
    14761 gtgcagatgg gacatttcca taagaaaaat gttcttcttg cactaatcca aggacattgg
    14821 ctgcacatgt atttgtaata gtcacttctt ctccgtcttc catcactacc ttgacgtaat
    14881 cagcagggtc tccttcttta ggagagaaaa attcaacctt aggacctttg attttttctg
    14941 tctccgtaaa tactgcataa tctagaccgg caatatgtgc agaacgtgga ccgacatcga
    15001 tgattcctga ctgattagcc cgtaccatcg aaccgcctgc cacacctaga acccgaacat
    15061 ctaatgaaga gatatatgtt gggtggccac ctacaattga ataatcgatt gctggacgtc
    15121 catttttaat gaccccgata ttggttgttg ttccgcctac ttcaaaataa acaccattag
    15181 acgctctcaa gtacatcaaa gagcccatga ctgatgctgc tggaccggaa agcatggtta
    15241 aaacaggtcg ttttttcatt tcattgattt ccattactcc gccgtctccg cgcataatca
    15301 tcaaagaaac attgacccct gcttctcgta cggaatcttc tgtagaggtt gccgtatcta
    15361 gcatttttgg taaaatagat gcattgattg ctgctgtccg agttcttctt gtcaatccat
    15421 ataattttgt gatgtcagaa gccatcgttg tagggatcga ttttaccgat gctgcttcat
    15481 aaacaactcg ttctggttcc ccattatcta ctccaaatgc catagaagaa accaagacct
    15541 ccgctctttc tctttctaaa gaagaaatcg tttctgaaac acggtctaca tttaagtgtt
    15601 tgacaggtaa gaatgcattg acaatctcta tttttttctt attccctaaa tcgatatcgt
    15661 tcaatctagt ttgtcgtttt gctaaaaaac cttctaatcc accttttgcc atcccgatta
    15721 cgccgacttt tgctacgtct ccttcaatca gtgcattagt cgcttgtgtg gtagaatgtg
    15781 caacaaacac aacatcttct ggactgatat tattttccag caaacaattt tgaaaagact
    15841 gaacgacacc tgcggctact ccacggacat catcgtgggt tgttttcact gaagattttc
    15901 cgatgatttc gtgcgtcgca ttatcgattg cgactgcttt cgtatgtgta ccgccaacat
    15961 caattcccat tcgcacaaaa cgtttcattt tcttttcctc cattcatcta atcgttttta
    16021 tcgacgtttt gtttactagg cgttcccgta caataagaac gtgatcaggc ataagatcgc
    16081 tccgatgatc caaccagttg ggatggattt cttcatatat tctcgagaag ttacttttgt
    16141 ataacctaat ccccaagcaa cccaagattg tgtaatatct aaatgctgag gagcaatcgt
    16201 tgtgattgca aataacggga acaaaaaggc tggcgacatc gttggattaa cagctaatac
    16261 gacagctaaa atagctgagc ctgaaccgac tagattcatt ggaccgcgga aaaatcctag
    16321 tggtgtcaat atagcaaata cgattgccaa gactaatggt gattgcggta tccaatctcc
    16381 aaaaatagct gaaaaataag gagaagcata cgcagctgaa ttattgaaca tcgctaaggt
    16441 catcaagaaa ccaaccattg gcgcaacatc tactgcgcca tctgtaaata atttcgcaaa
    16501 ctgacgacag atttctttat aacctccgcg caattttcca gtaaacagca atgcccagat
    16561 acctgctaga ataaaaccta agatgattgg aagatctaaa agaacgaccc ctaaaactgg
    16621 taataatacg gctaaccatg agatcattgg tgcgtcgcca ccttcagctg gtacgtttgc
    16681 tgccattgca tagcgtttct ttttgttcat tgatatatta gcgaccgtta gtaccacaac
    16741 taagcttaca atcatcccaa tcataccaat ttggaaatag tcattataag tatagctttc
    16801 agccgctgga ttgaaaccag cataaatcgt ttgatattgt ttgaaattga cgatattagc
    16861 aaagattcca gccataatcg agcccataaa tgagaacagt gcaaccggta ctgaaatacc
    16921 taatgacatc aagattggca acacaataac tgcaatagaa atcactggtc cgattcctgt
    16981 catcgacatg aatataacag cggttacaat acatagtaag ctcatcgtga ttcttggctt
    17041 gtcccctcca agttcaacta cctttcgaat cagggtagct gcgattccgc tatctactag
    17101 aacacgcccg aaaaaggcac caaagaagac atttaccaag atcgatttgg cgtactcagc
    17161 tggccccgtc gtatagatat gcgtcaacac atcaattact ccttgccctt ccatcgccgg
    17221 gtttggtgaa aacgtgtttc cgactagcgc taatcccatc catacagtag ccatgatggc
    17281 aaagccgacc atcaagttgt agccttttac acagtaccag accatgaaaa agaatgagat
    17341 aactaacaaa ataccaataa taatatcact catgttattc ctccgtccat tttgtttttc
    17401 tgataagcaa actaatatat cagtcaaact cattctaact tatgtagtta gcgctttcat
    17461 taaagggtga ccataaatgt tcagttttca attaaaaaac cttaattatg ctattttaga
    17521 cttatacgtt attgtcctgt ttcatttgat ggaggattgt aaataaaaaa agaggaaaga
    17581 acataacttt tcgttatctt ctttcctctt tataattgtt tagcttattt agccaaagct
    17641 tcttttgctt ggtcagccaa tgcagtaaat gctgctgcat cgttaacagc caagtcagct
    17701 aacattttac ggttgatgtc gatttcagca actttcaaac cgtgcattaa ttttgagtag
    17761 ctcaagccat tcatacgagc tgctgcgttg atacgagcaa tccacaattt acggaagtcg
    17821 cgtttctttt gacgacgatc gcgatatgcg tagttgtatg aattcattac ttgttctttt
    17881 gctgttttaa ataaagtgtg ttttgaaccg tagtaacctt ttgctaattt tagcactctt
    17941 ttacgacgtt tacgagttac tgttccacct ttaacacgtg ccatgtataa ttcctcctaa
    18001 taaaaataaa tcttcagatt ctgttatttg tacttcttat ttcatttttg ctagttgttg
    18061 gcgaatacgt ttgaagtcgc cactagaaac catgcttgct ttacgcaatt gacggcgttg
    18121 tttttttgtt ttgccgtgga aacggtgact tgtaaacgcg cggaagcgtt tcaatccgcc
    18181 tttaccagta cgtttgaaac gttttgctga tccgcggtgt gttttttgtt ttggcatgac
    18241 tatatttcct cctcaaaatc tttcgttatc cgactatcgg ctgtccaaca gccagttagt
    18301 cattcaaatt acttactgtc gtttttcggt gccagcgtca agaacatgct gcgtccgtcc
    18361 attttcgctt tttgttccac tgttgcaata tccgcagttt cttcagctaa gcgattcaag
    18421 actttttgac caatttcttt atgggtaatg gcacggcctt tgaaacggat agaagctttc
    18481 actttatcgc ccttttccaa gaacttacgt gcattacgaa gttttgtatt gaagtcattc
    18541 aagtcgatcg taggacttaa gcgaacttct tttacattga tcactttttg ttttttacgc
    18601 gcttcgcggt ctttcttttg ttgttcgaaa cggtatttac cgtagtccat gattcgcgca
    18661 actggtggtt tcgcaccagg ggcaactaac acaagatcca agtttgcttg ttctgcaata
    18721 ttcaatgctt ctgcttttgt cttaactcct aattgctcac catctgaacc gatcaatcgt
    18781 aactctcgtg cacgaatgcc gtcgttaacc atcatatcct ttgctatggt cattcacctc
    18841 caaatattat gagagaaaga aaagcttacc gaataaacgg caataaaaaa acgagttctc
    18901 ttacagaacc cgcgcgtact tcacctaata gaaccagtga aaactatcta gcccagtgac
    18961 attatcaact aaggcgagaa gcgggtgctt ctgcttttat ttctcaacca tcatagtata
    19021 gcaaaaacta atctttttgt caatgttttt catacagttt gtcttccttt tctgaaaatg
    19081 attagattag cagactttct tgcagtgatt cccgcttctt tgctatacta aagaaaaacg
    19141 aggtgaaccc acatgaaaaa aatcgaacct attccacctt ctcttcctct tcttacaaag
    19201 ggagagtttt atttagaaga tgaggagatc ttctctggtc ttctgatcaa cgggggtgac
    19261 caaagctatc tcgatgccaa aaatatggta ttaagagaga gtcatatcca aaaacttacg
    19321 atgcaaaaaa cagttttacg tcattttgaa tgcagtaatg tcatttttga aaaatgtgat
    19381 ttttccaatc tggagtggat tggtggcagt ttccaccaag tcgtgtttca tcaatgtaaa
    19441 ttaacaggta cgaattttgc cgaaagttat cttcgagact gtactttcac tgattgtatt
    19501 gcttcgatgg cttcctttag tagtacaaac ttgaaagctg tctccttcga tcattgtcaa
    19561 ttagaagaca gcgaatttta tgaggtcact tggaaaaacc tttttttgtc tgacaatcag
    19621 ttgaccggtt cgaactggtt tcgaacttct ctgaagggat tagactttac gactaatttc
    19681 ttcacagcaa tcgctttatc tcaagattct ttggcgggac taatcgtcaa ccaagagcaa
    19741 gcacttgtga tcgcccaagc ccttggtttg cagatcaaag actagatcaa cgaatccgcc
    19801 gccacagtgc aataagcatc caaatagata agaccaaact gatgatcaag gcgattcccc
    19861 atccgaaaac ggaatgggta aagggtaact ggagattcat tccgaagaat cctgtcacaa
    19921 tagttggaac ggtcaagatc agcgaccaga ccgtcaaaaa tttcatcgtg tcattcaagt
    19981 tgttattcag caaattatta taagtgccag agagctggtc taaaatctgc gaagctaagt
    20041 tcgtcatttc aactgcttgt cgtgcttcga tcagcgcatc gtccaactct tctttttcct
    20101 tttctgacaa cttgcgataa atagccaatg ccttgatttg ttctaaaagt actacatttt
    20161 gttttgttcc tgtaactaag taaaccaaac cgatctctaa atctgaaagt tgcagcagat
    20221 tcttgttcgt tgttttttct cttaatttca gattaagccg ctgacgttca ctattcactt
    20281 cctcgattaa aggaaaaaag gaatcagaaa tcaaaaacaa tgtacgaaac aataaactgt
    20341 atacactcat gtctggcatt cgttccaaaa gcgatcgaat tagtcgaatc acgtactcgg
    20401 tattcggcgt ggtaaatgta aacaaaccgt cttcttttaa aataaaagcg ataggactag
    20461 tttcataatg attatcgact ttttgctggt gcggaacatt tgatacaagc agtaacgctt
    20521 catcaaaaga atcgtactct acgcgcgccc gttcattttt atctagagaa taagtcagca
    20581 tctcgtcact aatgccatat tcttctttta agtttttaac agctagcaca tcgttttgct
    20641 gtacattgat ccaaaaatta cggtcttgat taaaatagtg tttttcccgc atactctatc
    20701 ctcctgacat ttgtttccag tataaagaaa aaagtcgctc aaataaaggt ttacccttta
    20761 tttgagcaac tgtaaatcaa atggctactt tggtttggcg aatctctgct aatttttctt
    20821 cgatcatttt caagacttct tcttttgctt gaggatcttc cacaaaatta tagcggtctc
    20881 catcgatttg gatttttgga ctttctttgt attcttcata ccattgatcg taacgtctgt
    20941 tcaattcttt gtaataatcg tatagacttg gatcaaagga caattgttca tacgaacgac
    21001 cacgtttttc gatacgctcc aacatcgtat caaaagatac acgaatatga actaataaat
    21061 ccggatgttt tttatgggca gcatagggaa gttcttccat catgttttcc agcagttcat
    21121 catacacacg aacttctgtt tctgttgctc ttcctagatc tgcatttaga tggaaaagca
    21181 gtgaatcttc ataaatcgaa cggtccaata cattattgtc atctttcatt gcttgtttga
    21241 tgcttgcaaa acgtttattc aaaaaataaa tttgcagcaa aaaagcatac tgttcagggt
    21301 ttgcataaaa taagggcaag acttcattat catcaatcga ttcataaaat gcctgactat
    21361 caaagtgttc agccatcatt tctgttaaac ttgattttcc ggcaccaatt gtacctgcta
    21421 aaacgatcac gctcatttac ctccagctca cttcatcact aaatattgtg tttaactcca
    21481 acctttttaa atataccccc aatatctagt agtgtcaatt gtattttttt ctagtcaaag
    21541 aagaaaaaaa gtcaaaaaat tcttaagaaa aaagtgtttt ttctttagaa aacgtattct
    21601 tagttttact tctgagatgg ttttagtaaa ataaaggagg aatgtttttt gagaccctta
    21661 gtggacaaaa aaattcgtga agcagcgcaa ctcgttttgg gcgctttatt ttttgcttaa
    21721 aaatcacttt tacgtgagaa ggagttagat tatggcaaac tatggccaag gagaatcaag
    21781 cggaaagatc atattgatgg gcgagcacgc ggttgtttat ggagaaccag cgattgcctt
    21841 tcctttctat gcaacaaaag tcaccgcatt ccttgaagag ctggatgcaa tggacgatca
    21901 actggtttct tcctactatt caggaaattt agccgaagct cctcatgcat taaaaaatat
    21961 caaaaaatta ttcattcact taaaaaaaca gcatgacatc caaaaaaact tgcaactgac
    22021 cattgaaagc acgattcctg ctgaacgtgg aatgggatca agcgctgcag tcgccacagc
    22081 agtcactcgt gctttttatg attacttagc atttcctttg tctcgtgaaa tactattaga
    22141 aaatgtccag ctttcggaaa aaatcgccca tggtaatcct agtggaatcg atgcagccgc
    22201 tactagcagc ttgcagccga tttattttac aaaagggcat cctttcgact acttttcttt
    22261 gaacatcgat gcttttttga ttgtcgctga tacaggaatc aaaggacaaa caagagaagc
    22321 cgtcaaagat gtcgctcacc tctttgaaac ccagcctcat gaaactggac aaatgatcca
    22381 aaaattagga tacttgacga agcaagcaaa acaagcaatc attgaaaatt caccagaaac
    22441 gttggcacag acaatggatg aatcacaatc acttctggaa aagctgacaa tcagcaatga
    22501 ttttcttaat ctattgatcc aaacagcaaa agataccggg gcattgggtg ccaaattaac
    22561 tggcggtgga cgcggtggct gtatgattgc attagcacaa acaaaaacaa aagcccaaga
    22621 aatcagtcaa gcacttgaag atgcgggtgc tgctgaaact tggatacaag gattaggagt
    22681 acatacctat gtttaaaggc aaagcacgcg catataccaa cattgctcta atcaaatatt
    22741 ggggtaagaa aaatgaagaa ctcatccttc caatgaacaa cagcctttcg ttaacattgg
    22801 atgcatttta tacagagaca gaagtcatct tttctgatag ctatatggta gatgaatttt
    22861 atctagatgg caccttgcaa gacgaaaaag caacaaaaaa agtcagtcag tttcttgacc
    22921 tttttcgtaa agaagccggc ctctcactaa aagcttcagt aatcagtcag aatttcgttc
    22981 ctaccgcagc tggattagcc tcctctgcca gcgggctagc tgctttagca ggcgcttgca
    23041 atactgctct taagcttgga ttagatgatc tctctctttc aagatttgct cgacgcgggt
    23101 ctggttcagc ttgccgaagt attttcggtg gtttcgtcga atgggaaaaa ggccatgacg
    23161 acttaagttc ttacgctaag ccagtccctt ccgattcttt cgaagacgat ttagcaatgg
    23221 ttttcgtttt gatcaacgac cagaaaaaag aagtgtccag cagaaatggg atgcgtcgga
    23281 cagtcgaaac atccagtttt tatcaaggct ggttagattc cgttgaaggg gatctatatc
    23341 aattgaaaca agcaatcaaa acaaaagatt tccaacttct cggagaaacg atggaaagaa
    23401 acggactaaa aatgcatgga acgactttag ctgcccagcc tccattcact tactggtctc
    23461 ctgacagctt aaaagcgatg gatgcggtgc gacaattgag aaagcaaggc attccatgct
    23521 actttacgat ggatgctggc cctaatgtca aagttttagt ggaaaacagc catttatctg
    23581 aggtacaaga aacatttacc aaattattta gcaaagaaca agtaattact gcacatgctg
    23641 gtccaggaat tgcgattatt gaataaaggg agatttttat gattgaagta tctgcaccag
    23701 gcaaactcta tattgccgga gaatatgcag ttgttgaaac aggccatcca gcagttatcg
    23761 ctgcagtcga tcaattcgtg acagtcactg tagaatctgc acgaaaagtc ggaagtatcc
    23821 aatctgcaca atatagtggg atgcctgtac gttggacaag acgcaacgga gaattagttt
    23881 tagatattcg ggaaaatcct tttcattata tccttgctgc tattcgcttg actgaaaagt
    23941 atgcacaaga aaaaaacatt cttttatcct tttatgatct gaaagtaaca agtgagttag
    24001 acagttcaaa tggccggaaa tatggattgg gttctagcgg tgccgtgacc gttgcaacgg
    24061 tcaaagcctt gaatgttttt tacgcgttga atttatctca gttggagatt ttcaagattg
    24121 cagcactagc caatttagca gttcaagata atggttcctg cggcgacatc gctgctagct
    24181 gttatggtgg ctggatcgct ttctcaacct tcgaccatcc ttggctccaa gaacaagaaa
    24241 ctcagcattc tatcagtgag ttacttgccc tggattggcc aggtctatcc attgagccat
    24301 tgattgctcc tgaagattta cgtttattga ttggttggac gggtagccct gcctctactt
    24361 ctgatttggt cgatcaagtt caccgttcga gagaagataa aatggtggct tatcagcttt
    24421 tcttaaaaaa tagtacagaa tgtgtcaacg aaatgatcaa agggtttaaa gaaaataatg
    24481 taacgttgat ccaacagatg attcgaaaaa accgacaatt actgcatgat ttatctgcaa
    24541 tcactggggt cgtcatcgaa acgcctgctt tgaacaaatt gtgtaattta gctgaacagt
    24601 atgaaggagc cgcaaaatct tctggtgcag gtgggggcga ttgcggaatc gtaattgttg
    24661 accagaaatc tggcattctt cctttaatga gtgcatggga aaaagcagaa atcactccac
    24721 tgccgttaca tgtctatagc gatcaaagaa aggaaaaccg atgaatcgaa aagacgaaca
    24781 tgtttcacta gctaaagcct ttcatgataa acaaaaaaac gaatttgact tcgtacggat
    24841 catccataac cctttgccgc aaatagcagt ttctgatgtc gatctaagta cacaggcagt
    24901 tgggtttaca ctaagcagcc cattttatat caatgcgatg actggcggaa gcgaaaaaac
    24961 aaaaaaaatc aaccaagatc ttgcaatcgt cgcaagagaa gcagatttga tgattgccac
    25021 tggttcagtc agcgctgcct tgaaagtccc ttctttagcc gatacttata cgattatgcg
    25081 acaagaatat cctcacggga aaatcattgc caacatcggt gcagggactt ctgtcgaaag
    25141 agccaaagaa gctatccgat tgtttcacgc agatgcctta cagattcatt tgaatgcacc
    25201 tcaagagttg gttatgcctg agggagaccg tgattttacc aattggaaag tacttattca
    25261 agaaactcaa acagccattg acgtccccct catcgtaaaa gaagtcggtt ttggcatgac
    25321 aagagaaacg ctcaatgatc tagctgcttt aggagttcat acagttgata tcagcggtcg
    25381 aagcggaaca agcttcaccc agatcgaaaa cgcccgtcgc tccaagcggg aactgagtta
    25441 tttagctgac tgggggcaat cgacagtctc ttcattgctt gaagcaaatg aagcagacac
    25501 ttctatggaa atcctagcct ccggaggtat ccgcaatgcc tatgacatat ttaaagccct
    25561 ttgtctcggc gcaaatgccg tgggtacttc aggaaccgta ctgacgcatc tgatgaatca
    25621 cggcgtagaa gaaactatta tactgatgaa gcaatggcaa gaagagcttc gtctgcttta
    25681 tactatggtc ggcgcaacga ataccgcggc cctacaccaa caatctctga tcttttccgg
    25741 accagtgaag gattggtgcg aagcaagagg gatcgactta gtgaagtatg gaagacgcac
    25801 agaaaagagc gtgggacaaa agtaaagaac acttttgtct cacgctctaa acacgtacaa
    25861 acggcggaag taaaagcaac tccttcggaa ataagctgaa attcacaaaa atttgaaaag
    25921 caattttcgt gaattccttc ttatttcttg gagttaaatg cttctgtccc agcctctttt
    25981 ttcgaataaa tgatgtttaa tcagctgtcc ttcggtattc gttcaatccg ctgatttcac
    26041 aacctctcca ctaaacagtg tactaaagct cttttaaggt tgttttccga tataggcaag
    26101 tatgcctccg tccacataca agacatggcc gttaacgaaa tcagatgcgc tagaagccaa
    26161 gaagacagat ggtccagcca aatcgattgg ctcgccccag cgtgctgctg gcgtacgtcc
    26221 aacgataaat tgatcgaatg gatgacgttc accgttttct tgtgtttcgc gtaatggagc
    26281 agtttgcggt gtagcaatat aacctggtcc aattccatta cattggatat tgtattgacc
    26341 gtattcagaa gcgatgtttt tcgtcagcat ttttaatccg ccttttgccg cagcataagc
    26401 actgaccgtt tcgcgtccta attcactcat catggagcag atattgatga ttttaccgcc
    26461 gcctttttcg atcatatccg gaataactgc ttttgaaacg atgaatgggg catttaaatc
    26521 cacgtcgatt acttggcgga agtctgccgc atccatttcg atcatcggtg tccgtttgat
    26581 gatccctgca ttgttgacta gaatatcaac tgaacctaca tcttctttga tctgttgaac
    26641 catcgcttga accgcttctt catcggtcac atcgcagaca taaccttttg catcgatgcc
    26701 tgcttcttta taatgcgcta taccttcatc aactgattct tgattcaagt tattaaatac
    26761 gatttttgct cctgctgtag ctaaagagcg tgcaatctca aatccaatac catagacagc
    26821 accggtgact aatgctactt ttccatctaa acgaaacata tccatactaa attccatcat
    26881 tttctctccc ttatgcttat tttaattgat ccatttgcac catatccatg tctgtatagg
    26941 tgatattttc tccgcacatt gcccagatga atgagtaatt gcttgtacct acacctgaat
    27001 ggatcgacca gcttggcgaa ataatggctt gttcattgtc tacaactaaa tgttttgttt
    27061 catctggttt gcccatcatg tggaacacac gtgtatcttc attggcataa tcaaagtaga
    27121 cataagcttc catacgacgt tcatgtgtat gacaaggcat cgtattccaa gcgcttcctg
    27181 gttctaaaat cgtataaccc atttgtaatt gacagctttc gcatacattt ggatggatgt
    27241 attggtagat tttacgttca ttcagtgtca ggccttcacc agtctccatt ggttttactt
    27301 ggtcgatact gatttttaca tttggatatt tatgatgagc tggaacagaa ctaatataga
    27361 atttagctgg gttttcagca tcttgagaag aaaatacaac atgtcgtgtt tcttttccga
    27421 tatagtatcc gtcttgtttt ttcatttctt ctttttgccc atcgatctcg atgaatcctg
    27481 gtccaccgat attgatcaca cctagttcac gacgctctaa gaagtaatca acacctagtt
    27541 ctttgtctaa tgtgatttct aattcttttt cagtaggtgt cactccacca aagatcattc
    27601 gatcgttgtg cgtataagtc aaactgattt tccctggttc aaataccttt tctactaaaa
    27661 attcttttct caattgttcc gttgaataat gacggatatc ttctggactg tgtgtataac
    27721 gtgtttccat ttcttgcatt ttcttctact ccttctatcg aatgattttt ccagagccgc
    27781 atgctaaaaa ttgtgcgact tcttcttgac taaattggtt gcagtcccca tggatcgtat
    27841 gcttcaaggc agaagctgcc gtagcaaaat caatgatttc ttgcggagac atattggata
    27901 aaatcccatg gagcacgcca cctgcaaaag catctccgcc acctactcga tccacgattg
    27961 gggtgattct atggacttgg gattccacat attcatggtt catccacaat gtccccttca
    28021 attcattttc acttgctgaa tgaacggtac gcttggtcga ataaaacacc tgaatattcg
    28081 ggaacttttc ctgcattttt tgataataat aaattagagg ttgttcttct gcttctggcg
    28141 ctttctcgat acctaacaaa taaagagcat ccatttttcc agctgagcag atatctacta
    28201 aaggtaaaag ctgatggatt acttcgctgg cttcttgctg tgtccataat tttccacgat
    28261 agttgatatc gaagctgatc aggcagccag ctttttttgc agcttctatc acctgtttcg
    28321 tcattttttg ccatttttta gacaaagctg gggtgattcc tgaaacatga aatatatcta
    28381 ttccttggaa taactcctct agcgaccatt ccaaatgatc catttcagca aaacttgatc
    28441 cggcacgatc atagatcact gaggcggcgc gttcgccact accagtttcc atataataag
    28501 tacctagtct tgatccacca gataatacct gtttcgtatc tactccataa cattgcaagt
    28561 gctttttgac tgcttttccc aatgcgttat caggtacttt tgtagcaaaa atcgcttcat
    28621 gtccgtaatt agctaaagaa attgctacgt ttgcttcccc tccgccataa tgggcagcga
    28681 atcgttcact tgaatcgata cgggttccct gctctgtgga caatcgcaac atgatttctc
    28741 cgagtgtcac tacttttccc ataagcagct acctcttgat ttctttcatt tttgctgcat
    28801 actgttgcgc gacttccgtt actttgtcaa aatctcctgt agctgcgggt gctaaaaggt
    28861 tcccacctac tccgactgtt acgacaccag catcgaacca ctctttcata ttgtccagac
    28921 tcacaccgcc agttggcatg atattgactt gaggcatcgg cgccttgaag gcagaaatga
    28981 tacttggtcc ataagcgctt ccaggaaata gtttgacgat atctaccccg cttttcagcg
    29041 ctgttttgat ctcggtaata gtcatacatc ctggtaagta aggaatttga tacagattgc
    29101 acatctctgc cgtttcttga tcaaaggaag ggctaacaat atactctgcg ccagctaaga
    29161 tagccaagcg agctgtgaca gcgtctaata ctgtgcctgc gccaatcact atttctggtt
    29221 ggtctttgta gaatgacaaa agttctttga ttacttgatc tgcttgaggg actgtaaaag
    29281 tcaactcaat accagttaga ccacctttga tgattgcgtg gcaagcattc aaagcttctt
    29341 ctttgctttt tccgcgtacc accgcaatca ctccggcgtt tttcaatcga gataaaatat
    29401 caattttttt catcttctcc acctatttcc tatttagaaa ttttatttta taacttggaa
    29461 atctataccc atattatacg gcagaaaaga aaaattgcaa acgctttttt cattattttt
    29521 gaattctttg ctcgatttcg ctttttgttt gcttgagggc actgattatt tgttcttttg
    29581 tctcatctgt taaacgatat ttcggcatac ttacactaaa tgcaccaatg atttgttcgt
    29641 ttttcacgat tgaggctcct atacaaaaaa tatcacgttc gatttcttca tcgtcaaatg
    29701 cgatgtgggt ttcttttacc gttttgattt cctttttcaa ccgtaatgca ttggtgatcg
    29761 tatattctgt gtaagggatc aacggatgct tttcaagata atcagtgtac tgctcttcac
    29821 tgaattctgc taatacggct ttgcccatcg cagaattgta taatggtcgg gtgattccga
    29881 tttttgaaga catgcggatc gtttgatgtt ttggatctag tttgttcacg tacaagatct
    29941 cattgttgtc caatacccct aagtggatag tctcatcgat cttttgctgc agctgttcta
    30001 aaaacggtaa agtcaactct accaaatcga tttgttcaat gtttttattc gcgtagcgaa
    30061 taagctttgc acctaaacga tagtttttct ctgaattttt atttacgtag ccgatcatca
    30121 acaaagtatc tagtattttc aagactgtag aagaggtcat tcccacaccc tttgcgatcg
    30181 tctgcaaaga gacatcttgg ttttccgcta aatagtctag tattttagag gctttgatca
    30241 atacagttcc ataaggcttt ttattttcca tataatattc tcctatttct ttataagaaa
    30301 aattatagcg gaaataccat caatttaaaa gagatatcca gatataaact accatttctc
    30361 tttacaggca atttaatgtt gaagatccgc ttcgttttta catcaaactt tgatataaag
    30421 caatgatttc ttctactgtc gtttctcttg gatttcctgg tgtgcataca tctcgcaaag
    30481 catcttctgc aattgctgga atatctgctt cttttacacc taattcagaa atggtttctg
    30541 ggattccaac cgctttgctc aaccgatcga tctcaccaca agccgcttct cttacatctt
    30601 ctaaagacat ttcagctgct cctttaatcc ctagaacaag agcgatctca cggtactttt
    30661 ctccagtata ttctttgtta tatttcatga cagttggcaa aagtgctgca cacgctacgc
    30721 catgaggaat gttgtaccat gcacttaacg ggtgagccat cccgtgtacc agtcctaaac
    30781 cgacatttga gaagcccatt cccgcaatat actgtcctaa tgccattttt tctcgcccat
    30841 tttgatcgcc atctacagaa gcttccaaag aatggccgat gatttcgatt gcttttaaat
    30901 gcagcatatc agtcatttcc caagcgcctt ttgtgataaa cccttcaata gcatgcgtca
    30961 tagcatccat acctgttgat gcagccaatt ttttcggcat tcccatcatc atatcgctgt
    31021 cgatgaacgc gacgattggg atatcatgag gatcaacaca aacaaatttt cggtgattta
    31081 cttcatctgt gatgacgtag ttgatcgtta cttctgctgc tgttccagaa gtcgttggta
    31141 tagctaatat aggtaaacat ggatttttcg tatcagcaac accttctaaa ctaatgacgt
    31201 ctgaaaactc tggattcgtc aaaataatcc caatggcttt tgccgtatcg attggcgaac
    31261 ctccaccgat tgcgatcaaa cagtctgcct ctgcctcttt aacaaaagcc actccatctt
    31321 ttacattttg gatcgttgga ttaggtacga tcccgtcgta taaagcataa gcgattgctg
    31381 ctttatctag aagttctgtt acttttgttg ctataccgtg ctcgatcaat cctttgtcag
    31441 taatgacagc tgctttgccg aatcctcgct gctgaaattc agggacgatc gactcaatcg
    31501 ctccttttcc aaaatacgat gtttcattta aaatcattcg attactcatt ttctattcct
    31561 cctaatttgt attatttttc acaataaaaa cgaaagaacc ccaagcaacc ggttgcccag
    31621 ttgcttgttg ctacagttca aagactggca tcaattccca actgactgga gaggcatctg
    31681 gatttgtctc catgatatct tccatgtact tccaccattt ttgattgata gatgtatttg
    31741 ccattttttc ccataacttt tcatcttcga tcactaagta accaaataac aaacttgtac
    31801 tcggatcaag atagatcgaa tagtcgatga ccccgtgttc acgaagtgct gtacgcattt
    31861 ctggccaaag ttcatcatgt ctttttttgt attcttcttg tttttctggg taaactttca
    31921 tgcaaaaagc ttttttcacc atgtcagaca cctgctttcg tatgaacttc ctccaaataa
    31981 cctgcttttg gagtgacacc aaacgcatct gctaaatgcc acaggttttc gtccgtgatt
    32041 gtttgcaaga ttggtccttg cgctttgacg tacgtataga cttctgctgc cttttctgct
    32101 gtctcgatca aaccaaagac ttcatccatg tcttttcctg acccgtagat tccatgttgt
    32161 ggccataaaa ccaatcgagt ttctttcatt ttttcagctg ttgcttcccc aatctcattt
    32221 gttcccggaa ccatccacgg aatgatggaa attccttctg gaaaaacaac taagcattcc
    32281 gtacacattt gccatagcgt acgactaaag gaacgttcgt ctaattcatg cgtaaaactc
    32341 atcgctaata gatgactagc atggcaatgc ataacgatcc gattatctgg gtctacttct
    32401 aaacgggcga tatgactcat gaaatgactt ggcaactcgc ttgtaggaag gcattcgttt
    32461 tccagccccc acaaaagttc cagtgttttg ccatcttcag caactcgaac gattccaaga
    32521 ttctcggcag gttcgctcgc tacattttta aaatatcttc ctgaaccagt tacaataaaa
    32581 tagcatcctg ctaaacggct ggcatcaaaa atcatcgaga tgcggcggat cactttttca
    32641 ggatccaaga acggtgtgat ctcttcttct tttagtaaat aagaaatatt cccgccattt
    32701 ctttcatccc atcccaagcg ataaagattc gacgtcgttt cgatcatttc tttcacataa
    32761 ggcgcttgta aaatatctat ttttttcaat atagccacac tcctattttc ttaattccaa
    32821 cacgtctttt tgatattcct ggacttcttc cagccaatcc aaaccaactg gaacttgatt
    32881 tttgtaacag aaataattcc atacatcggc atacgggaaa tctttcaatt cttccgtata
    32941 agccattctt gaagtaaagt caaaagccag ctccatttct ttcaatcgct caactggttc
    33001 cagcatagct ttcagcagtg ctttttgtgt atttctcgta ccgatgaccc aagcagcgat
    33061 acggttgatc gttgcatcaa agaaatccag accgatattt gttttttcca acaattcgtt
    33121 acggactaat tctttagcga tttcctgcaa ttcatcatcc atcaaaacca catggtctga
    33181 atcccaacgc actggacggg aaacatgcaa catgattcct ttgctgaata aagctaatga
    33241 agataattta ttggaaatca cttctgttgg atgaaaatgt ccagcatcta gacaaacaag
    33301 tttgtcacga gtcaacccgt aacccatata aaattcatgg gaaccgactg tataagcttc
    33361 agcgcctaaa ccaaatagtt tgctttctac tgcatcttgt acataccgtt catctaactc
    33421 ttctgtaaaa atttcatcga gtgattccat taaacgttcc cgtggggtaa aacgatcaat
    33481 cggattatct ttcatcccat caggtaccca aaagttattg atacttgttt gcccagtttc
    33541 tttcccaaag tactccgcga ttttgcggct tcttttcccg tgttcgatcc agtattcacg
    33601 aacttcttta tctggatgag ccaatgtaaa gccgtctttc atcattggat gcgaaaagaa
    33661 agtcggattg aagtcaagac cgatttcttc tttttttgcc cactctaccc atttttcaaa
    33721 atgtttcggc tcaatttcat ttagatcgac agattcttct gtatccaaat agatagcatg
    33781 aaggttcaat ttgtgtttgc ccggtatcaa cgaataagcc ttctccagat cttgtctaag
    33841 ctcttcaggt gttcgtgcgg ctcctggaaa attccctgtc gccatgattc caccggtcaa
    33901 ttcgccatcc ggtgtcaaga aacctttgac atcatctcct tgccagcaat gcatagaaat
    33961 tttgatgtcc tgtatctttt ctagtacagc atctgtatct acaccgatag ttgcatattt
    34021 ttcttttgct tgttcgtaat ttctttcaat cgtgttcata cgattactcc tttgatttta
    34081 ttcacgtacc attccatcgt gttttgctta ttttcatctg ggtaaaaagt ttcacatgga
    34141 aacgactgtt tgattacatt tcttgcttgt tctatattcg aaaactgacc tgtactgagc
    34201 atttgcatgg caacattccc gattgctgtg gcttcactag gtcctgcttc tacaatgatt
    34261 ccggcataat ctgctgtcaa ttgattcagt agacgattat tcgatccgcc ccctacgatg
    34321 tgcagctttt ccaatttcgt tccagttaag tgttctactt tgatcagctc ttgtgcgtaa
    34381 caaagagcaa gattatcata cacacaacga gcgatagctc ccgatgagac agggactggc
    34441 tgctctgttt caaaacagta ttgctgaatc tcttctatca tatttggggg attcaaaaat
    34501 cgagggtcat tgatttccat cacttgctgc agcggttttt cttttgcagc aagctctgcc
    34561 aattccgcat aactgtactc gtactcttgc attcgggcaa cttcctggat caaccacatc
    34621 cccatgatgt ttttcaaaaa acgcaccgtg ttatgggcac cccattcatt cgtataattt
    34681 tcagaaaaag cattgcgaga aacattggcg atctttgttt caatccctaa aagcgaccat
    34741 gtcccactgc tgatataacc ccaacgttct ccttgacctg gcgttcctaa tacagctgac
    34801 gccgtatcat gacttgctac tgtgataaag gtcacatctg gcaaatcata ttttgaaaat
    34861 tgttcttttt gcagatctcc tagtatcgtt cccggctctg tcaatggtgg aaataattcc
    34921 ttcgagacac cgattttttc aagaagttct atttcccatt cttttgtccc tgcattcagc
    34981 agctgcatag tagatgcatt agttttctcc gtcaccatgt taccagtaaa gacataccct
    35041 aaataatcag gtatcagtaa caatttatgt gcttttgcca aattttcttt ttcttcagca
    35101 aacaattgga acaaggtatt gaaaggctgg atctgaatcc ctgtctgctc atacaactta
    35161 tctagagaat agatttcaga aaactgttct actgctccat tggtacgctt gtcccgataa
    35221 gcaatcggat catttatccg atccccttgt tcatcaatca gacagtaatc gactgcccat
    35281 gtatcgatcc ccgcaaaaag gcgatcaatc cctgtctgct ttaatttttc aaatccaatc
    35341 aaaatctctt tgataagata atcgatcttc cagcgatcgt gaccgtcttt gttccgaaag
    35401 ccgtttttga accggtgtac ttcttccaag atcaaagaac cttttgcatc cctcgtactt
    35461 ttcattagtc gtccgcttga tgcaccgata tcgatggcaa caaatgttgg attcatttct
    35521 ccacctcttt tactttattg ttgattagca ctttattaat aaagcgttat catttattga
    35581 caatgctatc ataatacaga atcttatatt ttgcttcaca ctattttggt atataattgc
    35641 attatcttga caagttggga gtgtttacat ggattactgg gaaaaagcca accactcatg
    35701 gtcggaagac tcaaaaagag tgatcttgac gcctacacaa agaagcagga atttattttt
    35761 ttatatccaa gaaatcggtt acttcaaagc accaatccct tactatactg aacgagcaca
    35821 tttaccttct tacttgataa agtttacttt gtctggcaaa ggttcgctta catataatga
    35881 taatcagcaa cagctaaaag ctggagactt attttttatt gactgccaag aatatcagcg
    35941 ttatcaaacg atcagcgaag aaccatggga aatggattgg atccatttga atggtcagaa
    36001 cgtccaatca ttttatcagg agttcacaaa agacggccat aacaccttcc attcaacagg
    36061 agatccatca gaaaacaaga tccaccattt gatcacagaa ttgatccaca tgcaacaagg
    36121 cacgaatgcc cgtaccgatt tccagtcctc tgtagctatc cacgagttgc tgaatgaact
    36181 gattttgcaa aagtatcagc tagatttcga agaagaggat attcccgcct acgtttacaa
    36241 actgaagcaa tacttagacg aacatttcaa agaaacgatt acacttgatc aattagaaca
    36301 acaattctta ttgaataaat atcagctgag caaagatttt tccaaattta tcggttctcc
    36361 accaatcgat tatttgatca gcaaaaaact ttcttacgct aaagaccttc ttcggtatac
    36421 agaatttacg gttcgaatga tttccgaaga aatagggatt gagaattttg cttactttag
    36481 ccgcctgttc aaagcaaaaa caggactatc gccgagagac tttcgtaaat tcagctgatt
    36541 catataatat gtacgtcatt ttttcaaaat attttgaaat atatagataa aaataaaata
    36601 accgcagttt ctgcttctat gtaaacaaca gcagctactg cggttatttt ttagtgatca
    36661 ttctcattgt tcatcttcaa aatgttcata atgaaccaac tgtttctgtg tatcacgttt
    36721 caaaaattct ttaggcgtta tgccgaactc tctcttgaat gctttcagaa aatgcggata
    36781 agaagaaaaa ccacttttac tatatacagc actagagctg cggtattctc gcaacaagtg
    36841 cttggcatag atcagctttt tcttcattac atactgatga aacgtaaatc cagtgtactt
    36901 tttaaattct cgtgtgatat ggtatttgct gacaaaaaac tgcttttcca tttcctctaa
    36961 agaaagcgga tgagataaat tttttgagat atattgtaac atttgttcca cacgctcatt
    37021 aggaactaca gcattctgtt cttgcgcact ctcttctttt tgtacaactt gattgagaaa
    37081 aatcaagtat tctaccagag cttgttcata ttttatgtct tcaccgtaaa tttttttatc
    37141 aggaggatgg tcgataaatg ataatttttg cttcaatact tcctgttcta tttgcaatac
    37201 acgactcctc ttctctccca ttggttgaaa acaagccgat agattagacc atggcgtgga
    37261 acgactttct agaaaagaag gggtgacaaa gatatagaca cgctcgaata catctgaaga
    37321 ttggcggacg atacgatgca aatcattgga atggatgagc agcacacttc ctgctttgat
    37381 cgtaaattgt cgtccgttca aataaaaagt cgcttctccc tttaatgtag catggatctc
    37441 atagaaatca tgatgatgat agatcgtttt attttggctg acgacatctt tgacatgata
    37501 gatttcatac tgatcagaaa ccatcgtatt gacttgttca ccgctgattt tcttttggag
    37561 catgtgccgc acctcctttt gttcacttat gcacaatttt attataccac gttttttgta
    37621 atcaaaaaga gctgctttta tatactctta taaaaaagaa gagacgtggc atcttctagc
    37681 cccgccccct ccattctaaa tattagttaa ccaacgtctt ctttacatta ttcaagaaga
    37741 cttcttccat aacgaatccc atcatctttt gtttcttcaa gaaccacata caggtacgga
    37801 cgattcttca tgatgatttc tttgattttt gtgtactcga tcacaccgtc tcccaaattt
    37861 accggcacga ttttttcctg ttcgacacga aaatctttca aatgaaccgc acatatcttt
    37921 tcaccaaaac gttcaaaagc ttcttctacc aattggactt gttcttgata atcactactt
    37981 gtgatcaaat tggtcggatc taaaatgatt cctagatagt ccgaatctac tgcttggatc
    38041 agctgctcta cccttgctaa agaatacacc ggatgattca aacctggttc tatccccacc
    38101 atcatttggt atttttctcc agcttttacc aagcgacgga taacggacac agcttcagca
    38161 aatgcttcat ctgtgaaatt ttcttccgta tattggattt ccggcaaaac acatccagtt
    38221 tccgaagcaa ccatcgacgc accaaaggaa gaagcatagc gaagatactg ctcgaacttt
    38281 gtcagcaact cttgtcgtat cgttaaatct ggatgaatca tattgatata gcaacttaaa
    38341 attcctacgg tcacttcttg cttttctaaa actcgtttga cataatttcc catcccagaa
    38401 ttgatctcat ttgcgccaga aggtatttca ggaaacgaaa gacctaaggc gagctgcagt
    38461 gtatgaatcc cttgttcttt tgttttttct gcaagatctt ctatcgtttg gacttcggaa
    38521 agatcatgtc ctctaattgt aatgttcaac ataactattc tcctaactca gcatattggg
    38581 tagccacatg acgatttgcg ggaagtaagt aatcaaaaac aagacgacta agataacccc
    38641 ataaaaagga agaagcggtt tcagcattgc ctctgctttt acgcctccta cacttgcacc
    38701 gacatatagt cctgtaccta ctggcggcgt gatggagcct acacacagat tcatgattga
    38761 aaacaacccg taatgtacag gatcaacccc tacacttgtg acgattggta aaaatattgg
    38821 tgtaaaaatc aagatagcag gcgccacatc cataaacata cccaccagta ataacacaag
    38881 attgactaat aacagaataa taaatttatt atccgttacg cccataatca acgaactaat
    38941 tgcctgagga ataccggtaa acgccatggc gaaagacatc atggaagaag tagccaataa
    39001 gaacatgatc gtccctgaca ttgctactga ttgaaacaac atattaggta tatcttttag
    39061 ttttaccgtt ttatagtaaa acatagcaat caacagggag taaatgacag ctatcgctga
    39121 agcttctact gctgtaaata tccccgtcaa gataccaccg ataatgatga caatcaatag
    39181 aaggctagga atcgcctctt ttattatttt tccagcatga gccatctgtc ctttttcgac
    39241 aacagggtat ttattctttt tagcaacgat gaatgctact agcatgattc ctagcgccca
    39301 caaagcgcct acgagataac ctcccataaa caaagcagag atcgaagcac caccagcaac
    39361 aagtgaatac atgataaaag cagtacttgg aggaatgacc atacctgtcg gagctgacgc
    39421 gatattgacc gctgtcgcaa acttccggtc atatccttct tccacttgct gagggatcaa
    39481 cactccgcca atcgcggtag aagcggcaat ggccgagcta gagatggaac caaataaagc
    39541 attccctaac acattcgtat gagccaaagc ccccggcacg cgcccgacga acaacatcgc
    39601 aaaatccact agtttcttag ctatccctcc attattcatg ataatgcccg agagaacgaa
    39661 aaacggaata gcgatcagcg aaaaactatt gatacttccc accatttttt gagctgaact
    39721 aaaagcagaa acatcaaacg gtataactaa caataaagtc atcatcgatg agaatgcaat
    39781 acttatagca atcggcatcc ctaatacaag caacacaaca aatacaaaaa ataacactaa
    39841 tccggcttct attgccattt tattcctcca tttttatatt ttttcttgaa ttgctggctt
    39901 gttgcttaat gtatcgatta gattgatcag gctatagata atgatgagta cccccgatac
    39961 aggtactgac agataaacat atcccatagg aatacttaaa gaaggcgaga tctgccccat
    40021 tgtcaatgtg acagcattcc atccgccata caccatgatc atcgcagcaa ataccagaaa
    40081 taaactttga acaacaactt ctaaaatttt actttttttg ccagttaatt tttcacttaa
    40141 aaccgttacg gctaaatgtg ctttttgtcc cacaacatac gcggaagaca acatggcaaa
    40201 ccagacaaga ccgaatcgaa caagctcttc cgtgaccgta tttggatttc ctaaaaccgt
    40261 tctggaaaat acttgccata aaacaacagc gaccatccca acaagcaaaa cagctcccag
    40321 gatctctaat accttgttta aaatcatttt catcacagct acgctccttg ctcttggatc
    40381 ttttgataaa ggtctttgta ggtaggatcg tttttgaata tatcatgaag tggggcaact
    40441 gcctctcgaa acggtgtatt atctacatca ttgaattgga ctccatgttc ttcttgtgct
    40501 aaagcttttt cttcttcaat tgcttttgca aaagcttcaa tttcaaaaac cgttgattct
    40561 ttcgctgctt cttcaatggc tttttcctgc tcatcagtta gctgttcttt gatggcatca
    40621 ttcatgatga tgatatctgg cacacgtgta tgctcatcat aggaatagta tttagccaca
    40681 ccaccatgtc cagctgtata caagacaaat tcattgtttt ctgatccgtt gatcaaattg
    40741 gattgaagag acgtgtacac ttcatcactt cccataggta cgggcgaacc gcctaaaagc
    40801 tctaccattt tgatagcggt ttcgctttgc atcacacgga tcttctttcc cttcaaatct
    40861 tccggcgtat ggatcggacc gtctaccata taaaagctgc gctgaccaga atcataatag
    40921 gtcaatccgg taaatcctaa atttttggtc gattggtaga ttggctccat tatttcttca
    40981 ttatccatca ccttaaagaa atgttcttca tcattgaata gataaggaac tgaaaaaatc
    41041 gaataatctt tagagaaact ttccaacgct gaggcactta cttttgtgaa atctactgct
    41101 cctgactgtg tcaattcgat cagttcacgt tctccaccca gttgtccatc tgggaaatac
    41161 tggatctgta cggaaccatc tgttttttct tttactaaac gcccaaattc attcattgca
    41221 tcaattactg gctggctgtt gcttgcataa gcaaagcgca aggtaactat ctcatttaca
    41281 tctgcatctg cgacagttgc cacactagaa ttatttccgc agccgcttag acctagcaga
    41341 cctaggctaa gcgaacttgc tgcaaacagc agtgatgttt ttttcatatg ggtttccttt
    41401 cttttaacaa taagagacga gcaattctgt caaacaaagc accgttaacg actgtccata
    41461 aggcattgct gtgattccga tattccgata aaaatctaaa ctgtccccca ttcctgtccc
    41521 gattgaaacg ttttgaactt ctcctttttc atcgatttgt tcaagcaatc cttggatagc
    41581 acgataagcg acttctttat attcttgtgg taaataacgt ttatggaccg ctttcaaaat
    41641 accgtaagca aatcctgcag ttgcagaaga ttctacgtaa gaagaaggat catccaacaa
    41701 cgtatgccat aaaccgcttt cagactgata agtttttaat gcacgcactt gtgcttcaag
    41761 tgtttcgatc aataatttgc gcaatgcatc ttctttcgat aattctaaga tttcaatgat
    41821 ctctggaata gcaatcgtca accagcagtt tccacgagcc caaaatgctt cggcatagtt
    41881 atgatttcct tcaaacgtcc agccgtgata ccacaatcct gttcgtttat ccatcaagta
    41941 tttgatatgg atcataaact ggtaacgagc ttcttctaaa tattctgggc gattcaacag
    42001 tttcccgatt tttgccaaag gaagcactgt catcatcaat gtatcgtccc acaactgatt
    42061 cttattttcc ggtccatatg tggcatgctg taacccgtct tcatttgtcc gaggcatgtc
    42121 attcatgacc cattctgccc attgttctaa ataaggcaaa tagcgtgaat ctttcgtgtc
    42181 ttcatacaag taagccatag tcaatagcgg tgccattgta ttgacgtttt tgggcggagc
    42241 gccttcttcc atacgatcct caaaccattc attgaccact tgataagcgc gaggattttt
    42301 tgtcatctgg taatttttat aaataccata tagaccgaca ccttgtggcc aattccacac
    42361 tgtccagctt ttgtcatcta ctttcagtcc atcaaagtcc aacaaaaatt caccattatc
    42421 gtctttgatc gttgtcaggt tttcaacgag caggtcaatt ttttctttca ctaattcttt
    42481 aggtacaaaa atctcttctt taataatcat ctttacgcca cctttttaaa cgctttcatt
    42541 ttatatcctt aatatagcag taataccgtc cactataaat atcgcttttt gaacaaaaac
    42601 tgtctaaaat tgacctaaat gttaaataac aaaagtaact ttcagttact tggaaattaa
    42661 aaaaagaact tggagaaaca acgttttcca agttcttttt catctatttt cttatctttt
    42721 ttctacgctt tcttttgctt atccgttgta agcatcaatg atgatctgtt tcaattcaga
    42781 gatcaatggt tctttcggat tagctgtcgt acattggtct tcgtaagcta attcagccat
    42841 acggtctacg gttgtatcta gtgtttcttg tgtgactcct tgagctttta gactcatgtt
    42901 gatgccgaca gattttccta attcataaac agccgttgct aaagcttcga ctaattcctc
    42961 tgttgtttcg cctttcagac ctaagaattt tgcgatatct gcgtaatctg tatctgcacg
    43021 gaaatagtca tatttaggga acatcgcatg tttttgtgga tcttttgcat tatagcggat
    43081 aatatgcggt aaaagaatcg catttgtccg tccatgaggg atcccgtatt ccccgccgat
    43141 tttatgtgcg actgagtgac aaatacctag gaatgcgtta gcaaacgcca taccagccat
    43201 tgtagaagcg ttgtgcattt tttcacgtga ttccatatca ggtgttttta ctgatttttc
    43261 taaatagtca aagactaact tgattgcttg taggctcaat ccacgtgtat aatctgaagc
    43321 catgactgaa acataggatt cgattgcatg tgttaacaca tccatcccgg tatctgctgt
    43381 gacagatgct ggaactgaca tgacgaattg aggatcgacg attgcgacat ctggcgtcaa
    43441 tgcataatct gctaacggat atttcacatg tgtttcactg tcagtgatga ccgcaaatgg
    43501 cgttacttca gaaccagtcc ctgatgttgt cgggatacat acgaattgtg ttttttctgg
    43561 tttgtcgatc ttatatgtcc gtttacggat atccaagaat ttttgttttg caccaaagaa
    43621 agatgtgtct gggtgttcat agaacatcca cattccctta gctgcgtcca ttgcagaacc
    43681 gcctccaaga gcgattactg tatctggctt gaagtctgcc atcagtttcg ttcctgcgta
    43741 tacggtattt gtcgatgggt tcggttcaac atctgagaag acttcgatct tcacatcatt
    43801 tttacgtcgt gccaatactt cacgaactgt atcgcagtaa ccgaattgga ccataccagg
    43861 gtcacaaacg atcatgacac gttcaacgtt ttccattttc tctaagtaca atagagagtt
    43921 cttttcaaag aagatttttg gcggtaattt gaaccattgc atgttatttc tccgtttcgc
    43981 tacagttttg acattgatta agtttacagc agagacgtta cgagaaacag agttcttacc
    44041 ataagaaccg catcccaaag tcaatgacgg aatcatttcg ttatagatat caccgatgcc
    44101 gccttctgca gatggtgtat tcactaaaat acgacaagct ttcattcgaa gaccgaattt
    44161 gacatgcaac tcttcgtttt ctgaatggat aactgctgta tgtcctaatc ctccaagatt
    44221 caacatacct tcacacaaat caaacgcatg gtccgtgttg ttagctttga tcatggcaag
    44281 aactggagaa agtttttctc ttgatagcgg ataatctggg cctacgcctt ccaattcagc
    44341 aatcagcatt ttcgttcctt ttggtacact gattcctgcc aattttgcga tttccatcgc
    44401 agaatgtcca acgattgatg gattgaccgc atattttcct tcgttcatca ctgcatcttc
    44461 taatttttgc aattcatctg gttttacgat ataaacttgg tgtgcttgga attctgcttt
    44521 tactgctgca tagatttctt tatccacgat cacagcttgt tcagaagcac aaatcattcc
    44581 attatcaaat gttttcgaaa cgatcaaatc gttcactgca cgtttaattt ttgctgtttt
    44641 ttcaatataa gctggtacgt tacctggacc tacacctaaa gctggttttc ctgtagagta
    44701 agcagacttg accattccag ctccacctgt cgctaagacg atcgcgatac ctggatggtt
    44761 cattagcata gaagtcgctt cgatcgaagg atgttcgatc cattggatac aattttcagg
    44821 agctccggca gcgatagcag catcacgtac cactcgtgct gcttctgcag aagatttttg
    44881 ggcacttgga tggaaagcaa aaacgattgg atttcttgtt ttcaacgcga tcattgattt
    44941 gaaaatcgtt gtggaagtcg gattcgttgt tggagttact ccgcaaacca caccgactgg
    45001 ttcagcaatt tcaatcaacc ctttttggac atcttcattg atcacaccaa ctgttttatc
    45061 atgtttgata ttatgccaaa tatattcgga cgcatacata tttttgattg ctttatcttc
    45121 gtaaacaccg cggcctgttt cctctacagc catcttcgct aatggcatat gttgatccaa
    45181 agctgccatt gccatttcat gcacgatatg gtctactttt tcttgatcaa agctttccat
    45241 ttctttcaat gctgcatttg ctttttctgc caaatcatca atcattgctt gtacgtcaat
    45301 tgtcttcgtt ttttcttttt ctattacttt agccatcatt atcctcctaa tgggtcatgg
    45361 ttatttgtta aataattcac atgtatatca tacaatattt atttttctat gtaaacactt
    45421 tcttgtgatt tttttcacag ttatttttaa atataaaatt gctttattta caggtgtttg
    45481 tata
//
LOCUS       DAZKLM010000013        42798 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3764
            SAMEA115706913-rid22582563.denovo.013, whole genome shotgun
            sequence.
ACCESSION   DAZKLM010000013 DAZKLM010000000
VERSION     DAZKLM010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706913
            Sequence Read Archive: ERR13196647
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 42798)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42798)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287823.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287823.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,004
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,849
            CDSs (with protein)               :: 2,849
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 41 of 96
            Pseudo Genes (incomplete)         :: 52 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 15 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42798
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706913-rid22582563.denovo.013"
                     /isolate="VRE3764"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            352..1041
                     /locus_tag="ACG842_000651"
     CDS             352..1041
                     /locus_tag="ACG842_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFQ0247213.1"
                     /translation="MKILIADDDKEIVELLSIYVHNEGYEAVKAYDGKEALSKIRTIP
                     DIELLILDIMMPEMDGMQVVKELRKESQIPIIMLTAKTTDMDKIKGLVAGADDYVTKP
                     FNPLEVMARVKSLLRRTKMQIAPDQPDVLEVNALIINKDSHEVKTIDGKEIQLTALEF
                     GILYLLASHPNRVFSADEIFERVWKQESIVSAKTVMVHVSHLRDKIEEATGGEKVIQT
                     VWGVGYKIDAR"
     gene            1028..2236
                     /locus_tag="ACG842_000652"
     CDS             1028..2236
                     /locus_tag="ACG842_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HFQ0247214.1"
                     /translation="MTQDSKRATEKRRRINLTSKEISELLAEGIITIILLLLLNVSIL
                     VVISSVINSSPSLTNAIWDSKNIFAERLNTDLFWNGRNFIIPFFFLLDIGVLYWRLIR
                     RYRQMQLRHIISELHYIANGNYDHRIPFELSGDLSRVVTSINGLVDSTVAAIEDERKI
                     EKSKDELITNVSHDIRTPLTSIIGYLGLIEDGQYHSEEDLLKYTHTAYIKAKQMKSLV
                     DDLFEYTKVRQPAVPVNFSAFDMIQLIEQLAADFELEASKKNIQILVQSKVDSLIMDG
                     DTEKLVRVFNNLLTNALKYGKGATKIVIEVERIGSEVVATVKNNGAMIPQQAIDNLFD
                     RFYRVEESRSQATGGTGLGLAIAQSIVALHGGYIYAKSDKQWTSFIMHLPIKKNEKLP
                     IDTQEVIDES"
     gene            2530..3801
                     /gene="serS"
                     /locus_tag="ACG842_000653"
     CDS             2530..3801
                     /gene="serS"
                     /locus_tag="ACG842_000653"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HFQ0247215.1"
                     /translation="MLDVKMMRQNIEEVQEKLMTRGVKKETLAEFMELDENRRKLLVK
                     SEELKKYRNDVSAEIAQLKRAKEDASAKIAEMKEVGGNIKALDTEIAEVDEKLKQIAT
                     TLPNLPHDSVPVGTDEEDNVEIRRWSEPRTFAFEPKPHWEIAENLDILDFERGAKVAG
                     SRFVYYKGLGARLERAVYNFMLDQHIYEHGYTEMITPYIVNSKAMFGTGQFPKFKEDV
                     FQLENTDLTLIPTAEVPLTNYYNDEILDGADLPIYFTALSPAFRSEAGSAGRDTRGLI
                     RLHQFNKVEMVKFSDVEHSYEELEKMTADAEDILKKLHLPYRVMALSTGDMGFSAAKT
                     YDLEVWIPAQDTYREISSCSNCEDFQARRAMIRYRDNDGKIHYTHTLNGSGLAVGRTV
                     AAILENYQNEDGSVTVPEALVPYMGNLKVIK"
     gene            complement(4350..5834)
                     /gene="guaB"
                     /locus_tag="ACG842_000654"
     CDS             complement(4350..5834)
                     /gene="guaB"
                     /locus_tag="ACG842_000654"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HFQ0247216.1"
                     /translation="MSNWETKFTKKGFTFDDVLLIPAESHVLPNEVDMSVQLAKNIKL
                     NIPIISASMDTVTDSKMAIAMARQGGLGVIHKNMTISQQADEVRKVKRSESGVIIDPF
                     FLTPQHLVADAEELMSKYRISGVPIVETLENRKLVGIITNRDMRFVTDYHMPIADVMT
                     KDNLVTAPVGTSLKDAEKILQKHKIEKLPIVDNEGRLSGLITIKDIEKVIEFPNAAKD
                     EHGRLLVAAAVGVTSDTFERANALLEAGADAIIIDTAHGHSAGVIRKIQEIRSTFADA
                     TLIAGNVATAEATKALYDAGVDVVKVGIGPGSICTTRVVAGVGVPQLTAIYDAASVAR
                     QYGKAIIADGGIKYSGDIVKALAAGGHAVMLGSMLAGTDESPGEFEIFQGRRFKTYRG
                     MGSLGAMEKGSKDRYFQGSVNEANKLVPEGIEGRVAYKGSVADIIFQMIGGLRSGMGY
                     VGAANLQQLRDEAQFIQMSGSGLKESHPHDVQITKEAPNYSVES"
     gene            complement(6339..7034)
                     /locus_tag="ACG842_000655"
     CDS             complement(6339..7034)
                     /locus_tag="ACG842_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 domain-containing protein"
                     /protein_id="HFQ0247217.1"
                     /translation="MEPEQLRAIVKENNSLETKLTKRNQQYIFDLKKSLEAANLSEEE
                     KVKALHDILPTLVAEQKGGRTARQLFGTVSERTEAIINKPAEVKNSSTPLLMWLDNTL
                     LLLGLLSIMIAITGLFSRGTTPAYGFSTLVLGSAIGGWVFYMMYKYIYQYEYPGADRS
                     KKPGMGKTLLILGGTTLLWVVLFSATTLLPPVINPVLDPVVVIIIGATALAIRYYLKK
                     KYGIIGSLSVPRR"
     gene            complement(7124..8224)
                     /gene="ychF"
                     /locus_tag="ACG842_000656"
     CDS             complement(7124..8224)
                     /gene="ychF"
                     /locus_tag="ACG842_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002269933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HFQ0247218.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGAEAANYPFATIDPNVGMV
                     EVPDWRLQRLTELVHPKKTVPTTFEFTDIAGIVKGASKGEGLGNQFLSHIRQVDAICH
                     VVRCFDDDNITHVEGRVDPLEDIDTINLELVLADLESINKRYTRVAKVAKTKDKDAVA
                     ELAVLDKIKPVLEEGKSARTIEFTEEEQKIVKSLFLLTTKPILYVANVSEDDVADADA
                     NHYVQEVRNFAAEENAEVIVVSARAEEEIAELDEEDKAEFLEALGIEESGLDQLIRAA
                     YDLLGLATYFTAGEQEVRAWTFKKGIKAPQAAGIIHSDFERGFIRAETVSFEDLDHYG
                     NMHAAKEAGRVRLEGKDYVVQDGDVMLFRFNV"
     gene            complement(8233..8424)
                     /locus_tag="ACG842_000657"
     CDS             complement(8233..8424)
                     /locus_tag="ACG842_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HFQ0247219.1"
                     /translation="MYDLHDIVEMKKPHACQTNRWEVIRMGADIKIRCMNCGHIVMMT
                     RRDFEKKLKKVIEKNAEKE"
     gene            complement(8445..9335)
                     /locus_tag="ACG842_000658"
     CDS             complement(8445..9335)
                     /locus_tag="ACG842_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HFQ0247220.1"
                     /translation="MKKSKGLGRGIDALFQDLENLETVDVKDDTVVQLPLNELRPNPY
                     QPRKTFEEVSLQELANSIEQSGVFQPIIVRRSAVKGYEIIAGERRFRASKLAEKETIP
                     AIVRDFDEEAMMQVAVLENLQREDLNPLEEAEAYEMLMKNLKLTQAEVASRLGKSRPY
                     IANYLRLLSLPDLVKEMVQDQRLSMGQARTLLGLKNKTLILKLANRAVTENLTVRQLE
                     QLVSELNQNDGKEKKKIPRLAKEKPYYIRESEDRLMDKFGTSVAIQEKNGKGKIEIEY
                     LSQKDLTRILDILNIQFDEE"
     gene            complement(9322..10089)
                     /locus_tag="ACG842_000659"
     CDS             complement(9322..10089)
                     /locus_tag="ACG842_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HFQ0247221.1"
                     /translation="MAQIISVANQKGGVGKTTTTVNLGACLASLGKKVLLVDMDAQGN
                     ATSGVGIRKPDVTRDIYDVLVNELPIDEATLITEHENLSIVPATLQLAGAEIELTSMM
                     ARESRLKGSLAEVSSQYDYILIDCPPSLGHLTINSFTASDSILIPVQCEYYALEGLSQ
                     LLNTVRLVQKHFNPELEIEGVLLTMYDARTNLGNEVVEEVRKYFREKVYETIIPRNIR
                     LSEAPSHGKPIIDYDPRSRGAEVYQALAKEVVSREEK"
     gene            complement(10101..10814)
                     /gene="rsmG"
                     /locus_tag="ACG842_000660"
     CDS             complement(10101..10814)
                     /gene="rsmG"
                     /locus_tag="ACG842_000660"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HFQ0247222.1"
                     /translation="MIPEEFRQSLAEKGIELTDHQMNQFAIYYRTLVEWNEKMNLTAI
                     TDEKDVYLKHFYDSLTLAFEVDFTQPLSLCDVGSGAGFPSIPLKIAFPELQITIVDSL
                     NKRITFLSELTRLLGIDASLYHDRAETFGQNPHFRASFDLVTARAVARLNVLAELCLP
                     LAKQEGYFYALKAAKSEEELTEAKQAIALLGGKFLKEEAVTLPNVSDQRHIIVIQKKK
                     PTPKKYPRKPGLPNKQPIK"
     gene            complement(11046..12947)
                     /gene="mnmG"
                     /locus_tag="ACG842_000661"
     CDS             complement(11046..12947)
                     /gene="mnmG"
                     /locus_tag="ACG842_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HFQ0247223.1"
                     /translation="MEHYEADAYDVIVVGAGHAGSEAALAAARMGSKTLLLTINLDMV
                     AFMPCNPSVGGPAKGVVVREIDALGGEMGKNIDKTYIQMRMLNTGKGPAVRALRAQAD
                     KHAYSTEMKHTIEKEPNLTLRQGIVEELIVEDGVCHGVVTSTGASYRAKAVVITAGTA
                     LRGEIIIGELKYSSGPNNSQPSIGLANHLKELGFAIERFKTGTPPRVKSSTIDYSVTE
                     EQPGDKEPNHFSFSTPDSAYKLDQESCWLTYTGETTHQIIRDNLHRAPMFTGIVEGVG
                     ARYCPSIEDKIVRFADKPRHQLFLEPEGLNTEEVYVQGLSTSLPEDVQVNMLHSIAGL
                     EKVEMMRTGYAIEYDVVEPHQLRPTLETKVVENLYTAGQTNGTSGYEEAAGQGLIAGI
                     NAALKVQGKEPLVIKRSDGYIGVMIDDLVTKGTNEPYRLLTSRAEYRLLLRHDNADLR
                     LTEMGHRVGLVKEDQYKNYLKKKEAVEQEIKRLMNVRIKPTEEVQDFLATINAAPLKD
                     GVLASDFLRRPEITYAELLRFIPENEELTRKEIEQVEIQIKYEGYIKKAMEKVEKLKR
                     MEAKKIPENIDYAAINGLATEAKQKLQKIQPETIAQASRISGVNPADLSILMVYIEQG
                     KIAKVSEPA"
     gene            complement(12964..14361)
                     /gene="mnmE"
                     /locus_tag="ACG842_000662"
     CDS             complement(12964..14361)
                     /gene="mnmE"
                     /locus_tag="ACG842_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HFQ0247224.1"
                     /translation="MAEITLEFDTIAAISTPPGEGAISIVRLSGDQAVQLADKVYQSG
                     NKRLSEVPSHTIHYGHIVDPKSNQLVDEVMVSVMRAPKTFTREDVVEINCHGGIVVVN
                     QILQLLLREGARLAEPGEFTKRAFLNGRVDLSQAEAVMDLIRAKTDKAMGLALNQLDG
                     NLSALIRSLRQEILETLAQVEVNIDYPEYDDVEELTTKLLLEKAQMIQQRIQALLATS
                     KQGKVLREGLSTAIIGRPNVGKSSLLNHLLREEKAIVTDIAGTTRDVIEEYVNVRGVP
                     LKLIDTAGIRETEDVVERIGVERSRKALAEADLILLVLNQSEPLTAEDEQLLEATSGL
                     KRIILLNKTDLPAQLEQEKLKKLIENEPVFSISVAKNDGLDRLESAISDLFFSGETGE
                     RDATYVSNTRHIALLEKASLSLEEVIAGIDSGMPVDLVQIDMTRCWDYLGEVVGDSVQ
                     DELITQLFSQFCLGK"
     gene            14660..15043
                     /locus_tag="ACG842_000663"
     CDS             14660..15043
                     /locus_tag="ACG842_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0247225.1"
                     /translation="MVALSKLYTFERRKYVSPFRSAALEEESDPRINDVVLSTGNINQ
                     KYIVRDIVFVVECLEADLFDPSFPKDDLFLTTKRKLKTKVLEYGANAVINCHFEYQQK
                     KRDGKYFLELFAYGTVIQFIQTTIG"
     gene            complement(15211..15969)
                     /gene="jag"
                     /locus_tag="ACG842_000664"
     CDS             complement(15211..15969)
                     /gene="jag"
                     /locus_tag="ACG842_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding cell elongation regulator Jag/EloR"
                     /protein_id="HFQ0247226.1"
                     /translation="MPIYEAATIDEAVQLGLADLGLAKDQVEIEILEEGKKGFLGMGR
                     KNARLSIQPSISEKVSETVEQTVEEILEEEPTAEIEVVNETILEEKTEEVSELGDEAA
                     LTELAVYLTEISKQLDAPALVKMERESNNMVVFHLETQKQGILIGKHGKTLNALQYLT
                     QVFIHRLARNKLSVVVNVGNYREKRQEIIQRLADRTAEKVKRTGQPVFLEPMPAFERK
                     QIHAALSKDEHVKTHSEGEEPYRYLVVEPAKKYY"
     gene            complement(15987..16805)
                     /locus_tag="ACG842_000665"
     CDS             complement(15987..16805)
                     /locus_tag="ACG842_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YidC/Oxa1 family membrane protein insertase"
                     /protein_id="HFQ0247227.1"
                     /translation="MKKYKRLLLMAGIVSLVIVLSGCGTGEVSAQSTGIWDRYIVYYF
                     AEAIKALSFGNAGIGIILFTLIIRVILLPLMHFQTKSMRKTQELQPKLKALQQQYSSR
                     DPETQSKLREEQQRLYAENGVNPYAGCLPLLVQMPILMALWQSISRVPELKQGHFLWL
                     NLGNADPTFILPILAAIFTFASTYLSSMSQIESNASMKIMNFVMPVMILVMGVNLASG
                     LSLYWVVSNAFQVVQTLLINNPFKIRKEREEEARRIRERERALKKAMNPKKKRK"
     gene            complement(16802..17158)
                     /gene="rnpA"
                     /locus_tag="ACG842_000666"
     CDS             complement(16802..17158)
                     /gene="rnpA"
                     /locus_tag="ACG842_000666"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HFQ0247228.1"
                     /translation="MRKAYRVKKEREFQAVFHTGDSFANRKFVVYRLEKPGQPHFRVG
                     LSVGKKVGNAVNRNEVKRKIRAAVYSIKEEIDPELDFIVIARPSVKGLTYDEVRSNLV
                     HVLKLAKIIDREEKSK"
     gene            complement(17276..17410)
                     /gene="rpmH"
                     /locus_tag="ACG842_000667"
     CDS             complement(17276..17410)
                     /gene="rpmH"
                     /locus_tag="ACG842_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003397591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HFQ0247229.1"
                     /translation="MKRTYQPNKRKRQKVHGFRKRMSTKNGRRVLASRRRKGRKVLSA
                     "
     gene            17933..19267
                     /gene="dnaA"
                     /locus_tag="ACG842_000668"
     CDS             17933..19267
                     /gene="dnaA"
                     /locus_tag="ACG842_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HFQ0247230.1"
                     /translation="MVSLDALWNELKATYQKDLSPASYNTWIETANPRTLDQNQLVVE
                     VPSKIHKEYWEKNLATKIVEVGYMLSGNEIIPRFITGEEAEQEEVIEEKNPKVVAPSP
                     LKKAMLNPKYTFDTFVIGKGNQMAHAAALVVAEDPGSIYNPLFFYGGVGLGKTHLMHA
                     IGHQMLQSQPNAKVKYVSSETFANDFINSIQNKTAEEFRQEYRNVDLLLVDDIQFFAE
                     KEATQEEFFHTFNALYNEGKQIVLTSDRLPNEIPKLQERLVSRFAWGLSVDITPPDLE
                     TRTAILRKKAAAERLEIPDDTLSYIAGQIDSNIRELEGALVRVQAFAAMNSEDISTSL
                     AADALKTLKSGKGHPQLSILQIQEEVAKYYHIQLKDLKGKKRVKSIVVPRQIAMYLAR
                     ELTDNSLPKIGAEFGGKDHTTVIHAHEKIQQLMDSSVSMQNEVSEIKNFLLN"
     gene            19465..20595
                     /gene="dnaN"
                     /locus_tag="ACG842_000669"
     CDS             19465..20595
                     /gene="dnaN"
                     /locus_tag="ACG842_000669"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HFQ0247231.1"
                     /translation="MKVTLNRASFMQELQTVQRAISSKTTIPILTGVKITLTQEGLTL
                     TGSNADISIETFLSVENEKANMQIESTGSIVLQARFFSEIIRRLPEETFTLEVLENKQ
                     VAITSGKANFIVNGLDADNYPHLPVVESHNQMKLPVHVLTKLINETVFAVSQHESRPI
                     LTGVHFILSDNSLLAVATDSHRLSQRVIPVEQAADHFDIVIPGKSLIELSRSLTNEEE
                     IVEISIMENQVLFKTETMYFYSRLLEGNYPDTNRLIPSSFNTEVEFSVPSFLAAIERA
                     SLLSHEGRNNIVRLSIRPDAVVLYGNSPEIGKVEESLSYTASSGDPLDISFNPDYMKA
                     ALRAFGDMSIKVKFISAIRPFTLEPTEDGVQFIQLITPVRTN"
     gene            20824..21066
                     /gene="yaaA"
                     /locus_tag="ACG842_000670"
     CDS             20824..21066
                     /gene="yaaA"
                     /locus_tag="ACG842_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HFQ0247232.1"
                     /translation="MKQQVILNTEFMTLGQMLKEVTVIGSGGQAKWYLAENTVLVDGE
                     PENRRGRKLYPGMMVEVPEVGTFFMVKKGNDDNEAE"
     gene            21053..22177
                     /gene="recF"
                     /locus_tag="ACG842_000671"
     CDS             21053..22177
                     /gene="recF"
                     /locus_tag="ACG842_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HFQ0247233.1"
                     /translation="MRLNKLYLKNYRNYEELNIEFSKNLVIFLGENAQGKTNLLESIY
                     VLAMTRSHRTSSEQELIGWTDDQAMIQGEITKGSSTLPLEILLSKKGRKTKINHIEQK
                     KLSTYVGQMNVILFAPEDLSLVKGSPQIRRKFLDMELGQINPIYLYDLVQYQAILKQR
                     NQYLKQLNEKKQTDLLYLDILSEQLAAFGSKVLKARAQFVKRLEFWANSLHQQITHQK
                     EQLEIEYLTAVDSLETHTQEQIQEQFLALLNQNKKKDLFRGTTTVGPHRDDLSFFINQ
                     KNVQTYGSQGQQRTTALSVKLAEIDLIKEETGEYPILLLDDVMSELDDNRQLHLLETI
                     EGKVQTFLTTTTLDHVKNKMTVEPEIFYVQQGKLKGEETE"
     gene            22174..24120
                     /gene="gyrB"
                     /locus_tag="ACG842_000672"
     CDS             22174..24120
                     /gene="gyrB"
                     /locus_tag="ACG842_000672"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003129249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HFQ0247234.1"
                     /translation="MTEERSLVERAKEYDASQIQVLEGLEAVRKRPGMYIGSTSSEGL
                     HHLVWEIVDNSIDEVLAGFATKIHVIIEKDNSITVIDDGRGIPVDIQAKTGRPAVETV
                     FTVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTQLDVKVYKEGNVYYQEYRRGAVVD
                     DLKIIEQTDRHGTTVHFTPDPEIFTETTEFDFSKLATRIRELAFLNRGMRISIEDKRE
                     EEPVINEYHYDGGIKSYVEYLNANKTVIFPEPVYLEGEQQDIAVEVSMQYTDGYHSNI
                     MSFANNIHTYEGGTHESGFKTALTRVINDYARKQKLMKENDDNLTGEDVREGLTAVIS
                     IKHPDPQFEGQTKTKLGNSEVRTVTDRLFSEHFMKFLLENPSVGRQIVEKGLLASRAR
                     LAAKRAREVTRRKGALEISNLPGKLADCSSNDPEKCELFIVEGDSAGGSAKQGRNREF
                     QAILPIRGKILNVEKASMDKILANEEIRSLFTAMGTGFGEDFDVSKARYHKLVIMTDA
                     DVDGAHIRTLLLTLFYRYMRPIVEAGYVYIAQPPLYGVKQGKNITYVQPGKNAEEELK
                     QVVASLPASPKPSVQRYKGLGEMDDHQLWETTMDPSNRMMARVSVDDAIAADQIFEML
                     MGDRVEPRRAFIEENAHYVKNLDI"
     gene            24140..26611
                     /gene="gyrA"
                     /locus_tag="ACG842_000673"
     CDS             24140..26611
                     /gene="gyrA"
                     /locus_tag="ACG842_000673"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HFQ0247235.1"
                     /translation="MSEEIRENIHDVNLTSEMKDSFIDYAMSVIVARALPDVRDGLKP
                     VHRRILYGMNELGVTPDKAHKKSARIVGDVMGKYHPHGDIAIYESMVRMAQPFSYRYM
                     LVDGHGNFGSVDGDGAAAMRYTEARMSKIATEMLRDINKNTVDFQSNYDDTEKEPVVL
                     PARFPNLLVNGTTGIAVGMATNIPPHNLSEVVAAIDLLMDNPDVTTNELMEVLPGPDF
                     PTGGLVMGKSGIRRAYETGKGSITVRAKVEITEMPNGKERIIVTELPYMVNKAKLIER
                     ISELHRDKRIEGITDLRDESSREGMRIVIDIRRDASASVILNNLYKLTSLQNSFGFNM
                     LAIEKGVPKVLSLKQILENYIEHQREVITRRTVFEKEKAEARAHILEGLRIALDHIDE
                     IIAIIRGSKSDDEAKATMIERFDLSDRQSQAILDMRLRRLTGLEREKIENEYQELLKL
                     IEDLTDILARPERVTEIIKNELADLNQRFGDKRRTELLVGEVLSLEDEDLIEEEEIVI
                     TLTNNGYIKRLANNEFRAQRRGGRGVQGMGIHNDDFVKNLVSCSTHDTLLFFTNNGKV
                     YRAKGYEIPEYGRTAKGIPIINLLGIDSSESIQAIIAVTGEAEEGHYLFFTTRQGTVK
                     RTSVTAFANIRSNGLIAIGLKEDDELVNVLLTDGQSNIIIGTHNGYSVTFKEEAVRDM
                     GRTASGVRGIRLREEDYVVGAALMKENQEVLVITEKGYGKRTKVSEYPVKGRGGKGIK
                     TANITEKNGPLAGLTTVSGDEDIMLITDKGVIIRFNVSTVSQTGRSTLGVRLMKMEAD
                     TKVVTMAAVEPEAAEQTVEEQGSEE"
     gene            26847..27146
                     /gene="rpsF"
                     /locus_tag="ACG842_000674"
     CDS             26847..27146
                     /gene="rpsF"
                     /locus_tag="ACG842_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005226363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HFQ0247236.1"
                     /translation="MENTKYEIMYIIRPNIDEEAKTALIERFDAILKDNGAEVIESKD
                     WEKRRFAYEMNGYREGIYHIVKVSSPSSANAVNEFDRLAKINDDIIRHMIVKEEA"
     gene            27196..27708
                     /gene="ssb"
                     /locus_tag="ACG842_000675"
     CDS             27196..27708
                     /gene="ssb"
                     /locus_tag="ACG842_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFQ0247237.1"
                     /translation="MINNVVLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQSGNR
                     EADFINCVIWRKSAETLANYARKGTLLGVTGRIQTRSYDNQQGQRVYVTEVVADNFQL
                     LESRSASENRQQSGGFQSSGQSAGGFGGNNNSNQTSQSSNGMPDFDRDTSDPFGSSST
                     IDISDDDLPF"
     gene            27734..27970
                     /gene="rpsR"
                     /locus_tag="ACG842_000676"
     CDS             27734..27970
                     /gene="rpsR"
                     /locus_tag="ACG842_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HFQ0247238.1"
                     /translation="MAQQRRGGRKRRKVDYIAANHIEYIDYKDIELLKRFISERGKIL
                     PRRVTGTGAKNQRKITVAIKRARIMGLLPFVSED"
     gene            28113..30086
                     /locus_tag="ACG842_000677"
     CDS             28113..30086
                     /locus_tag="ACG842_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HFQ0247239.1"
                     /translation="MENKNNHLSPKPLFLILFILIEVLVVLILPFRFVSASVILLMNL
                     WGVRYWLKMKRWLILNKREKIQQASTVAETNLAYVSEVMPVGVIAYQPSSQKIEWLNP
                     LALKIKDQSGLTEEELLDTFFTAKEKKKNQVYLADKTFQFDMDTVKGVVYFNDITQNQ
                     LLKLHQTNSQPVIGILSIDNYADAIDKLDDKEISYLNSFVTTLVSDWMNEYHVFFKRI
                     NAERFFFVAQMIDLKKMMDKKFDLLDRFRAEAEKQSSPITLSMGIAYGSRTLDEIGGE
                     AQNNLDVALVRGGDQVVVKEVKEGAKPVYYGGKSAKNVKRTRVRSRAMSTALRRIFDE
                     TGDVFIMGHKFPDMDALGSAFGVSCLARFNQKKAYIVINENEIIPDVERCLEEIHKHP
                     ELEMQLISPERAIELKKEEDLLVMVDYHKPSLSISQGLYEQFDKIVIIDHHRRGEEFP
                     SKPLLTYIESSASSASELVSELIQYQSSKTNRLDRFEATLLLAGMVVDTNSFNVRTTA
                     RTFDVASYLRSCGADASLVQYLLSSDLATYLEISNLIAGSDYVTSDIVVAAGTDEKEY
                     NSVIAAKTADTLLSMININAAFVITKRTDGLIGISARSTGTINVQIIMESLGGGGHFT
                     NAATQLENTTVEETKQKLFAAIKENMNQIYDKE"
     gene            30092..30544
                     /gene="rplI"
                     /locus_tag="ACG842_000678"
     CDS             30092..30544
                     /gene="rplI"
                     /locus_tag="ACG842_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HFQ0247240.1"
                     /translation="MKVIFLQDVKGKGKKGDIKEVPAGYAQNYLIKNNLAKEATKSSI
                     SQLAGQKKAQEKHEAEILEEAKKLKEFLEKEDTVVELKAKAGEDSRLFGSIPSKQIAE
                     GLNKQYKIKLDKRKIELENPIRSLGYTNVPVKLHHEVTATIKVHVVQE"
     gene            30818..32185
                     /gene="dnaB"
                     /locus_tag="ACG842_000679"
     CDS             30818..32185
                     /gene="dnaB"
                     /locus_tag="ACG842_000679"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HFQ0247241.1"
                     /translation="MNEVWQDRIPPQNIEAEQAVLGSVFLDAETIIDAMEYIEPKDFY
                     RRGHQIIFETMIELNDRNEAIDVVTVKDRLEQANLLEDAGGLSYLSDLALAVPTAANI
                     VYYAKIVEEKSLLRTLIQTATGIVTKGFEQGEEVQTILDDAERSILEVSEKRNRSGFL
                     SIAEVLNSTIENIDRLAQNNEEITGLPTGYQGLDKMTAGFQPEELIILAARPAVGKTA
                     FALNIAQNVGTKTDRSVAIFSLEMGAESLVNRMLCAEGSIEASHLRTGQLSEEEWSNL
                     IVAMGSLSRANIFIDDTPGIKISEIRAKCRKLAQEKGNLGLILIDYLQLIEGNGKENR
                     QQEVSDISRQLKKLAKELKVPVIALSQLSRGVEQRQDKRPVLSDIRESGSIEQDADIV
                     AFLYRDDYYEREGGDDDDGNEPKNDNVIEVIIEKNRSGARGTVELLFIKEYNKFSSLS
                     PREEF"
     gene            32965..34257
                     /locus_tag="ACG842_000680"
     CDS             32965..34257
                     /locus_tag="ACG842_000680"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HFQ0247242.1"
                     /translation="MSSVVVVGTQWGDEGKGKITDFLSENAEVIARYQGGDNAGHTIK
                     FNGVTYKLHLIPSGIFYKDKISVIGNGVVVNPKSLVKELAYLHEKGVETSNLRISDRA
                     HVILPYHIKLDQLQEDAKGENKIGTTIKGIGPAYMDKAARVGIRIADLLDKEIFEERL
                     RLNLDDKNRQFVKMFDSEPLDFDDIFEEYYEYGQQIKQYVTDTSVILNDALDAGKRVL
                     FEGAQGVMLDIDQGTYPFVTSSNPVAGGVTIGSGVGPSKINKVVGVCKAYTSRVGDGP
                     FPTELFDETGEQIREVGREYGTTTGRPRRVGWFDSVVMRHSKRVSGITNLSLNSIDVL
                     SGLETVKICTAYELDGELIYHYPASLKELNRCKPIYEELPGWSEDITGCKTLAELPEN
                     ARNYVRRISELVGVRISTFSVGPDRTQTNILESVWAQI"
     gene            35044..35907
                     /locus_tag="ACG842_000681"
     CDS             35044..35907
                     /locus_tag="ACG842_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HFQ0247243.1"
                     /translation="MYQIVTDSCCDLPYHVLAKEKVDFLSMNLQLNDQELVDDLGKTF
                     NYDRYMNQLKNGAMPTTSQINVGRYLEFFRSYAEKQIPVLYVAFSSGLSGSYSSALQA
                     VELLKEEYEDPEIYVFDTKAASIGEGLLVLEAVRLKKEGKSIEETLEWLESNYLKVHS
                     WVTVDDLKYLERGGRISKASATLGSLLSVKPIITVDKEGRLTNVDKVRGRNKSIQTIV
                     NETVKTIVAPLDQIVYVAYAGDLESAEKAKRLLEEQIKMKDVKLYPLGPTIASHTGYG
                     CIAIFSMGISR"
     gene            complement(36036..37658)
                     /locus_tag="ACG842_000682"
     CDS             complement(36036..37658)
                     /locus_tag="ACG842_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HFQ0247244.1"
                     /translation="MITVNDVSLHFSDRKLFDDVNIKFTPGNCYGLIGANGAGKSTFL
                     KVLSGDLQPSTGSVTMGPDERMAVLKQNHYDYEDYTVLETVIMGHKRLYEVLKEKDAI
                     YMKEDFTDEDGIRAAELEGEFAELNGWEAEPEAAVLLQGLNIPEELHHQQMSELTGGQ
                     KVKVLLAQTLFGKPDVLLLDEPTNGLDIQSINWLEEFLINFENTVIVVSHDRHFLNKV
                     CTHMADLDFGKIKLYVGNYDFWLESSQLAAKLQANANAKKEEQIKELQEFIARFSANA
                     SKSKQATSRKKMLEKITLDDIQPSSRRYPFVGFKPEREIGNDLLQVENVSVTIDGKKI
                     LDDISFNLSKEDKVAFVAENDITTTTLFKVIMGEITPDTGTVRWGVTTSNSYLPKDTT
                     KEFDTDLTILDWLRQYASKEEDDNTFLRSFLGRMLFSGEEVLKPVNVLSGGEKVRCML
                     SKMMLSKANVLVLDDPTNHLDLESITALNDGLMAFTGSILFASHDHQFIQTLANRIIA
                     VSDKGVIDRADTTYDEFLENADIQKQLNELWA"
     gene            37907..38428
                     /locus_tag="ACG842_000683"
     CDS             37907..38428
                     /locus_tag="ACG842_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HdeD family acid-resistance protein"
                     /protein_id="HFQ0247245.1"
                     /translation="MDEKRTIDWGSLILGILFVLVSLLSFQDPVGNLVAIVVVFAIFA
                     FIKGIFELFVRNRLKELTGYKGKMPLIIGIIDILVGVFFLFNIGAGVAALPFVFAVWF
                     IADSVLALFTADLARGVSEGYYWFTIIVNILGILLGIFLLFNPISSALTLSFLVGFYF
                     MLFGITHIVYAFR"
     gene            complement(38614..40344)
                     /gene="spxB"
                     /locus_tag="ACG842_000684"
     CDS             complement(38614..40344)
                     /gene="spxB"
                     /locus_tag="ACG842_000684"
                     /EC_number="1.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047112 - pyruvate oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate oxidase"
                     /protein_id="HFQ0247246.1"
                     /translation="MSKINAGIAMVKVLESWGIDHIYGIPGGSFNSIMDALYQEKTEI
                     NYIQVRHEEVGALAAAADAKLTGKVGAVFGSAGPGATHLINGLYDAQMDHVPLVAFLG
                     QVASTSMNYNSFQELNENPIFADVSVYNRTVMTPQSLPHVVDEAIKAAYEHKGVAIVT
                     IPVDLGFEEIDEQPFSTAHTHKTSVILPEEKDLLAALPYLEKAKKPVLYVGQGTRNGF
                     PQIKEFSEHFSVPVVASVLAKGIIPDDYENFLGFAGRVATKPGNEALAEADLILFVGS
                     DFPFGRAFFNPTAEFIQIDIDASKFGRRHETSLSILGDANTALQRLVELGDARPADSW
                     YLANQENKEKWVNWLKSFEDREEEPIRPEAVYKEINRIAEDDAIFVTDVGNTTIHSIR
                     LLNMNGKQKHTTSGWFATMGNGVPGGIAAQLSYPEKQVFTLSGDGGFAMVMQDIITQV
                     KYQLPIINVVFSNDSFGFIEAEQEDTEQKKFGVFLEDADFGKVGEALGANSFTVTEYS
                     QLRFAFDAAAKSDRPVVIDVKINNKRPLPVEDLHLDPRKYSEEEIQAFKEKYEVHDLP
                     VLNELYEKRL"
     gene            40509..41327
                     /locus_tag="ACG842_000685"
     CDS             40509..41327
                     /locus_tag="ACG842_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HFQ0247247.1"
                     /translation="MVEWLLRLEPWQQALAGTAFTYFMTALGAGLVFFFKEIKKEVLN
                     LMLGFASGVMIAASFWSLLDPAITKAEENGDIAWLVVSIGFGLGGVFLYMADKTLPHM
                     HFGPQHEAEGLPTHLKRTILLVFSITLHNIPEGLAVGVAFGAAATADNPTAAILAAVS
                     VALGIGIQNFPEGAAVSIPLRQEGLSRKKAFVYGQASGIVEPIAGVIGALLVTKVELL
                     LPYALAFAAGAMIYVVVEELIPEAQQTLSSKRHFAVFGVMSGFIIMMILDVALG"
     gene            complement(41430..41627)
                     /locus_tag="ACG842_000686"
     CDS             complement(41430..41627)
                     /locus_tag="ACG842_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0247248.1"
                     /translation="MRQKNMFLNIFMVLLVLIAASFVISLFVSVLSGLLWFAVKVLIP
                     IALIIWLVRWIKGNTHRKNYY"
     gene            41796..42374
                     /locus_tag="ACG842_000687"
     CDS             41796..42374
                     /locus_tag="ACG842_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071805 - potassium ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM175 family protein"
                     /protein_id="HFQ0247249.1"
                     /translation="MPKNRLEAFTDAVIAIIMTILVLELHEPNGDTWEALAALGYRFF
                     IYIVSFIILAIYWNNHHHLFQVVHKINGRVLWANSFFVFALSLFPFATSWISEYINSL
                     APEMTYGFVTLTANIAYYILAKELVKANKGHEKALKAMLNNYKKSYLSIGLNVLGIVL
                     GYLVAPVAVLIANIVILAAWIIPSRTIESQYR"
     gene            42469..42606
                     /locus_tag="ACG842_000688"
     CDS             42469..42606
                     /locus_tag="ACG842_000688"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0247250.1"
                     /translation="MDQKRIHVGLITSFVTMLIYLDQGHLTWTVVGVVGFLTLFTAIK
                     K"
BASE COUNT        13015 a         8209 c         8467 g        13107 t
ORIGIN      
        1 tttccaacat gtcaacaact tattttttat tatttaataa gttattggtg ttcttttgaa
       61 caaataataa tatatcaaca tcacatgtga aagtcaataa gaaaatgctt atttatcaac
      121 atttctagct tacatgatca ttggttagtt ctggtccttt ttcgttcgtc tttataactc
      181 cgagagacgt aaaatccttt tgttttcagt ttaatgttca tgttcttgtc ttattcacac
      241 caactagatg tctcaccttc taaaattttc cttaacaaag tgctgctttt atctatttct
      301 gctttttttt gctagaataa tagtaacaat atgactctgg aggatttatt tatgaaaatt
      361 ttgattgctg acgatgataa agaaatcgtt gaattattaa gtatctatgt tcacaatgag
      421 gggtatgaag cagtaaaggc ttatgatggg aaagaagcat tatctaaaat cagaacaatc
      481 cctgatattg aattacttat tttagatatc atgatgcctg agatggatgg gatgcaagta
      541 gtgaaagagc tacgaaaaga atcccaaata ccaatcatta tgctgacagc aaaaactact
      601 gatatggata agatcaaagg actggtcgct ggtgcagatg attatgtgac caagccattt
      661 aatccgttag aagtaatggc tagagtaaag tctttattac ggcgtacaaa aatgcagatt
      721 gccccagatc agcctgatgt attagaagta aatgcattaa tcatcaataa agactctcat
      781 gaagttaaaa caatcgatgg gaaagaaatc cagttaacag ctttggaatt cggtatcctc
      841 tacttgcttg ctagtcatcc taatcgagta tttagcgcgg atgaaatttt tgagcgtgtt
      901 tggaaacaag aaagtattgt ctctgctaaa actgtcatgg ttcacgttag tcatttaaga
      961 gataagattg aagaagcaac tggtggtgaa aaagtcattc aaaccgtttg gggagtgggc
     1021 tataaaattg acgcaagata gtaaacgagc aactgaaaaa cgccgtcgga tcaatttgac
     1081 ttcaaaagaa atcagcgaat tgttagcgga aggaatcatc acgattatcc ttttgcttct
     1141 attaaatgtt tctattctgg ttgttatcag ttcggtgatc aacagttcac cttctttgac
     1201 gaatgcgatt tgggattcaa agaatatttt tgcagaacga ttgaacactg atttgttctg
     1261 gaacggccgg aattttatta ttcccttttt ctttctatta gatattggtg ttttgtattg
     1321 gcgcttgatt cgaagatatc gccaaatgca attgcggcat atcatcagcg aacttcacta
     1381 catcgcaaac ggaaattatg atcatcgtat cccttttgaa ctgagtggtg acttaagccg
     1441 agtggtaaca agtatcaatg gattggtgga tagtaccgta gcagcgattg aagacgaacg
     1501 taaaatcgaa aaatcaaaag atgaattgat cacgaatgtc agtcatgata tccgtacccc
     1561 actcacgtcc atcattggat acctaggact gatagaagat ggccagtatc actcagaaga
     1621 agatctttta aagtatacgc atactgctta tatcaaagca aagcagatga aatctttagt
     1681 ggacgactta tttgaataca ctaaggtacg ccagcctgct gtcccggtga atttttctgc
     1741 ctttgacatg atccagttga tcgaacagtt agcagctgat tttgagttgg aagcttctaa
     1801 aaaaaatatc caaatacttg ttcaatcaaa agtagactcc cttatcatgg atggcgatac
     1861 cgaaaaactt gtacgtgtct tcaataatct tttgacgaat gcgttgaaat acggaaaagg
     1921 agcaacgaag atcgtaatcg aagtagaacg tatcggttca gaagttgttg caacagtcaa
     1981 aaataacgga gcaatgatcc cacaacaggc aatcgataat ctattcgacc gtttttatcg
     2041 tgtagaagaa tctcgttcac aagcaactgg aggaactgga cttggtttag caatcgctca
     2101 aagcattgtg gctttgcacg gcggatacat ttatgcaaaa tcagataagc aatggacttc
     2161 tttcatcatg caccttccaa tcaagaaaaa tgaaaaatta cctattgata cacaggaagt
     2221 tattgacgaa agctgaaata ttcgctattc ttattacaat tattagaata aatgaaaaac
     2281 acagaaccca gtacacttta ttctttttca gagagctgat ggcaggtgta aatcagcaaa
     2341 ggataatttg gaagttccag ttcgtttacg tgccctttcg aaataagaaa ggtcggttca
     2401 acagccgtta tgtgttcaac aagtgcagct gacgctgaac ttgggtggta ccgcgaattt
     2461 cagactgatt cgtcccaaga ataatgggat ggatcagtct ttttttattt tcaaaaagga
     2521 ggaaatcata tgttagatgt aaaaatgatg cgacaaaata ttgaagaagt acaagaaaaa
     2581 ttgatgactc ggggcgtaaa aaaagaaaca ttagctgaat tcatggaact agatgaaaat
     2641 cgtcgtaagc tactagtcaa aagtgaagaa ttaaaaaaat atcgaaatga tgtttctgct
     2701 gagatcgccc aattgaagcg tgcaaaagaa gatgcttctg ctaaaatcgc tgaaatgaaa
     2761 gaagtcggtg gcaacatcaa agcattggat actgaaattg ctgaagtgga tgaaaaattg
     2821 aaacaaattg caactacttt gccaaacctt ccccatgatt ctgtgccagt tggaacagat
     2881 gaagaggaca atgtagaaat ccgtcgttgg agcgaaccac gtacatttgc ttttgaacca
     2941 aaaccacact gggaaattgc agaaaatctt gatattttag attttgaacg tggcgcaaaa
     3001 gtagccggta gccgttttgt ctattacaaa ggattaggtg ctcgattaga acgtgctgtc
     3061 tacaacttca tgcttgatca gcatatttac gaacacggct atactgaaat gatcactcct
     3121 tatatcgtca acagtaaagc gatgtttggt acgggacaat ttccaaaatt caaagaagat
     3181 gttttccaat tggagaatac tgatctaaca ttaatcccaa cagctgaagt accattaact
     3241 aactattata atgatgaaat tttagatggt gcagatttac ctatttattt cactgcacta
     3301 agccctgctt ttcgttctga agccggaagt gctggtcggg atacacgagg actgattcgt
     3361 cttcaccaat tcaataaagt agaaatggtc aaattttcgg atgtagagca ttcttacgaa
     3421 gaattggaaa aaatgacagc tgatgcagaa gatatcttga aaaaattgca tttgccttat
     3481 cgtgtgatgg ccttatcaac tggagacatg ggcttttctg cagctaaaac ttatgactta
     3541 gaagtatgga tacctgcaca agatacttat cgtgaaatca gttcttgttc taactgtgaa
     3601 gatttccaag cacgccgcgc aatgatccgt tatcgcgata atgatggaaa aatccactat
     3661 actcatactc tgaatggttc aggacttgca gttggccgta cagtagctgc cattttggaa
     3721 aactaccaaa atgaagatgg ttctgtcaca gttccagaag cgctcgttcc ttatatgggg
     3781 aatctgaaag ttattaaata aagaggctgt gaaaaagccg cttagtttcg agtattaaga
     3841 aaaattttgt gaaaatggct ttccacattt ttgcataaat tggacttaat accgagaaac
     3901 tggcttttga acaccattta tcgagaggtt gtgaaatcag cgaattaacc tgagtattag
     3961 agaaaaaaat ggaaaaatag tccctcatat tttaccgttt gttgaactaa taccgagggt
     4021 cagctgattg aacaccgttt agtggagagg tcgtaagcct accccccagc tcctagttac
     4081 aaggacaaat tcaaaaaaca gctcttcata ttttatgaat gttgtacttg tgattcggag
     4141 gtggcaaggg aacactgttt attcaaaaag aggctgggac agaagcgttt aactccaaga
     4201 aataagaaga aattcacgaa aattgctttt caaatttttg tgaatttctt cttatttccg
     4261 aaggagttgc ttctacttcc gccgtttata cgtgtttaga gcgtgagaca aaagtgttct
     4321 ttacttttgt cccacgctct ttaatgtttt taagattcaa ctgaatagtt tggtgcttct
     4381 tttgtgattt gtacatcatg agggtgtgat tcttttaatc cactgccgct catttggata
     4441 aattgcgctt catcacgtaa ttgctgtaga ttggctgccc caacataacc cattcctgaa
     4501 cgcaaaccgc cgatcatttg gaaaataata tctgcaacac ttcctttata agcaacccga
     4561 ccttcgattc cttctggtac taatttattt gcttcattga cactaccttg gaagtaacgg
     4621 tcttttgaac ctttttccat cgcgcctaat gagcccatac ctcggtatgt tttgaatcga
     4681 cgtccttgga agatttcgaa ttcacctggt gattcatctg tccctgccaa catacttcct
     4741 agcatcactg catgtccgcc ggcagctaat gctttgacga tgtcaccaga atatttgatc
     4801 cctccgtctg caataatcgc tttgccatat tgacgagcaa ctgatgctgc atcataaata
     4861 gctgtgagct gaggtacacc tacaccggct accacacgtg tcgtacaaat cgatcctggt
     4921 ccgataccaa cttttacgac atctacacct gcatcatata aagcttttgt cgcttcagcc
     4981 gtcgcaacgt ttcctgcaat caatgttgca tctgcaaaag ttgaacggat ttcttgaatt
     5041 tttcggatca cacctgcact atgtccatga gctgtatcga tgatgattgc atctgcccct
     5101 gcttctaata aagcattcgc acgctcaaat gtatcgcttg ttacaccaac ggctgctgct
     5161 acaagcaatc gaccatgttc atcttttgct gcatttggaa attcgatcac tttctcgatg
     5221 tctttgatcg tgatcaatcc gcttaaacga ccttcgttat ctacgattgg taatttttcg
     5281 attttatgtt tttgcaaaat cttttctgca tcttttagag aggtacctac tggggcagtc
     5341 actaagttgt cttttgtcat cacatcagca atcggcatgt gataatctgt tacaaaacgc
     5401 atgtcgcggt ttgtaattat ccctaccaat tttctgtttt caagtgtttc aacgattggc
     5461 acaccactga tacgatattt gctcatcaat tcttctgcgt ctgctacaag atgctgtggc
     5521 gttaagaaga acggatcaat gattacgcca ctttcagaac gtttgacttt tcttacttca
     5581 tccgcttgct ggctgatcgt catgttttta tggatcacac ctagtccgcc ttgtcttgcc
     5641 atagcaatgg ccatcttgct gtcggtaaca gtatccatac ttgcactgat gattggaata
     5701 ttcaacttaa tatttttagc taattgtacg ctcatatcca cttcgtttgg taacacgtga
     5761 ctttctgctg gtatcaataa gacatcatca aaagtaaatc cttttttcgt gaactttgtt
     5821 tcccagttgg acatttttag taccactcct ctaatttagt ttatctatta aaataattga
     5881 ttttttcatt taagtcaatg atctacttgg aatcaagtta gctttctcat agaataccaa
     5941 tataaagcgt ttacatctgt gtgtattaaa agagattgta aaaaagctgt cctgcatcaa
     6001 gtaataagaa cagccaatca aaaacagctt ctcatgtttt tgaattggtt gggtttatta
     6061 ccgcagatgc aaacttttga acaccgttta gtggagaggc tgtgaaaaag ctgcttagtt
     6121 tcgagtagta agaaaaattt tgtgaaaatg gctttccaca tttttgcata aattggactt
     6181 aataccgaga aactggcttt tgaacaccgt ttatcgtaaa aagggactgt gacaagactt
     6241 ttgtcacagt cccttactat tatttaacta tggataaaca aaatgaggtt ctttactacc
     6301 cctagatatc tagcaaaata gctgatcttt ttgctagttt atcttcttgg gacggataaa
     6361 cttccaataa tcccgtattt tttcttcaag tagtaacgga ttgctaaagc agttgccccg
     6421 ataatgataa ctactactgg atctaatact ggattgataa ctggtggcaa tagtgttgtc
     6481 gcagaaaaca gtacgaccca aagaagtgtc gttccaccta ggataagcaa tgtcttaccc
     6541 attcctggtt tcttgcttcg atctgctccg ggatattcat attgatagat gtatttgtac
     6601 atcatataaa agacccagcc accgattgca gaacctagaa cgagtgtaga aaagccataa
     6661 gctggcgttg ttccacgtga aaacaatccg gtaatcgcaa tcatgatact taatagacca
     6721 agcaacagca acgtattatc taaccacatc aataaagggg tggatgaatt ttttacttct
     6781 gctggcttat tgatgattgc ttccgttctt tcagagactg tcccaaacaa ttgacgggct
     6841 gttcttccgc ctttttgttc tgcaactaaa gtaggcagaa tgtcatgaag ggcttttact
     6901 ttttcttctt cagacaaatt cgctgcttct aaagattttt tcaaatcaaa aatgtactgt
     6961 tgatttcttt tggttaattt tgtttctaaa ctattgtttt cttttacgat tgctcgtaat
     7021 tgttctggtt ccattatcta atagtcctcc ttagccgttc cttttctcaa cggtcgtcac
     7081 aaaagacatt ctaccatatt ctacagctag tttgatagcc cttttacaca ttaaagcgga
     7141 ataacattac gtcgccgtct tgtacgacat aatcttttcc ttctaatcga acacgtccag
     7201 cttcttttgc tgcatgcatg tttccgtaat gatccaagtc ttcaaaagag acagtttctg
     7261 cacggataaa tccgcgttcg aaatctgaat ggatgattcc tgctgcttgt ggtgctttga
     7321 ttcctttttt gaatgtccaa gcacgtactt cttgttcccc agcagtaaag taagtagcta
     7381 agcctaacaa atcataagca gcacggatca actgatccaa acctgattct tcgattccta
     7441 aagcttctaa aaattccgct ttgtcttctt catctaattc tgcgatttct tcttctgcac
     7501 gagcactgac cacgatcact tctgcatttt cttctgcagc aaagttgcgc acttcttgta
     7561 cataatgatt cgcgtctgca tctgctacgt catcttctga gacattggct acataaagaa
     7621 ttggttttgt cgtcaataaa aatagtgatt taacgatttt ttgttcttct tcagtaaatt
     7681 cgatcgttcg ggcagatttt ccttcttcca gtacaggttt gattttatcc agtacagcta
     7741 gttctgccac tgcatcttta tctttggttt tagcaacttt tgctacacga gtatagcgtt
     7801 tgttgataga ttctaagtct gctaacacta gttccaagtt gatcgtatca atatcttcta
     7861 gtggatcaac acgtccttct acgtgagtaa tattatcatc atcaaaacag cgaacaacgt
     7921 gacaaatagc gtctacttgg cggatatggc ttaagaattg atttcccaaa ccttctcctt
     7981 tacttgcgcc ttttacgatt ccagcaatat ctgtaaattc aaaagtcgtc ggaacagttt
     8041 ttttcggatg tacaagttct gttaagcgtt gcaaacgcca gtcaggtact tctaccatcc
     8101 ctacgttggg gtcgatcgtc gcaaagggat agtttgctgc ttctgctcct gcttttgtga
     8161 tcgcattaaa caaagttgat tttcctacgt tgggcaaccc aacaattcca gcagtcaatg
     8221 ccataaattt attcactctt tctctgcatt tttttcaatt acttttttta atttcttttc
     8281 aaaatctcga cgggtcatca tgacgatatg tccgcaattc atacatctga ttttgatatc
     8341 tgcgcccatt cgaatgactt cccagcgatt cgtctggcaa gcgtgaggtt ttttcatttc
     8401 aacgatatcg tgcaagtcgt acataaaaat agcctccttt ttgtttattc ttcatcaaat
     8461 tgaatattca agatatcaag aattcgagtc aaatcttttt gagaaagata ttcaatctcg
     8521 atcttacctt tcccattttt ttcttggata gctacacttg taccaaattt atccatcaaa
     8581 cgatcttcgc tttctcggat ataatatggt ttttcctttg ctagccttgg gatttttttc
     8641 ttttcttttc catcattttg atttaactca gaaacaagct gttccagttg gcggacagtt
     8701 aaattttcag ttactgcacg attggctaat ttcaagatca atgttttatt ttttaaacct
     8761 aacagcgtac gggcttgtcc catagataaa cgctgatctt gtaccatttc cttgacaaga
     8821 tccggtaaag aaagcaagcg caaataattg gcgatataag gtctgctctt tcctaaacga
     8881 ctagctactt ctgcttgtgt tagtttcagg tttttcatca gcatctcata tgcttctgct
     8941 tcttctaacg gattcagatc ttctcgctgc aagttttcca gcactgctac ttgcatcatc
     9001 gcttcttcgt caaagtctct aacgatagca gggatcgttt ctttttcagc tagtttggac
     9061 gctcgaaaac gtcgctcccc tgcaataatc tcatatcctt ttactgcgga acggcgtacg
     9121 atgatgggtt gaaagacacc tgattgttca atagaattag ctagttcctg caaagatact
     9181 tcttcaaatg ttttacgggg ctgatacgga tttggtcgca gctcattcaa tggtagctgg
     9241 acaacggtat catcttttac atccaccgtc tctaagtttt ctaaatcttg aaacagtgca
     9301 tcgattcctc ggcctaatcc tttacttttc ttcacgactt accacttcct ttgccaatgc
     9361 ttggtagact tcagctcctc gagaacgtgg atcgtagtcg atgatcggtt ttccatgact
     9421 aggtgcttct gataaacgaa tatttcttgg aatgatcgtt tcgtaaactt tttcgcggaa
     9481 gtatttacgt acttcttcta cgacttcatt tcccagattt gttcgtgcat catacattgt
     9541 cagtaagact ccctcgatct ctaattctgg attaaagtgt ttttggacta aacgaaccgt
     9601 attcagtaat tggcttaatc cttccaatgc gtaatactca cactgaacag gtatcaaaat
     9661 cgaatcactt gctgtaaatg aattgattgt cagatggcca agagaaggcg ggcaatcgat
     9721 caaaatataa tcatactgac tgcttacttc tgctaaagat ccttttaatc ttgattctct
     9781 tgccatcatt gacgtcaatt cgatctctgc tcctgctaat tggagagtcg caggcacaat
     9841 actcaaattt tcgtgttcag tgatcaaagt tgcctcatca attggcaatt cattgactag
     9901 aacatcatag atgtctcttg tcacatcagg ttttctgatc ccgacaccac tggtagcatt
     9961 tccttgtgca tccatatcca ctagcaatac ttttttgcca aggctagcaa gacaagcacc
    10021 tagattcacc gtcgtcgttg ttttgccgac gccacctttt tggttcgcaa cagatattat
    10081 ttgtgccatc tcttctcctc ctatttgatt ggctgcttat taggcagtcc gggctttctt
    10141 gggtatttct ttggggttgg tttctttttt tgaataacga tgatatgccg ttgatcagag
    10201 acattcggca aggtaaccgc ctcttctttg aggaatttac cacctaataa agcaatcgct
    10261 tgttttgctt ctgtcaattc ctcttcactt tttgctgctt tcaatgcata aaaatagcct
    10321 tcttgtttag ccaagggcaa acacaattcc gctaacacat tcaaacgagc aaccgcacga
    10381 gcagtgacaa ggtcaaatga tgcacgaaag tgaggatttt gcccaaatgt ttctgcccgg
    10441 tcatgatata aggaagcatc gattcctaat aatcgggtta actcagataa gaaggttatt
    10501 cgtttattca gagaatcaac gatcgttatc tgcagttctg gaaaggcaat ttttaaaggt
    10561 atgctaggaa accctgcccc cgaaccaaca tcgcataaag ataaaggttg cgtaaagtct
    10621 acttcaaatg ctaatgtaag tgaatcgtaa aaatgcttca gatatacgtc tttttcatcc
    10681 gtaatagctg taaggttcat cttttcgttc cattcgacta atgtccgata ataaatagcg
    10741 aattgattca tttgatgatc ggtaagctct atcccttttt ctgccaagga ttggcgaaat
    10801 tcttctggta tcataaatat tcctttcatt gtgaaacagc aattgtttca cagattctct
    10861 ccctacttta acgcaaaatc tctagacatg caatccttcc atatggattc ttaaaaattt
    10921 gaaaagtaag aaaagccgat agactttttc tttctagtat gactcataaa atatacacag
    10981 aagagagaac tagctttgag caatttctgc aaaaactagt tctctcttct atttattatt
    11041 acgtattacg ctggttcact gactttcgca atcttccctt gttcgatata gaccattagg
    11101 atacttaggt ctgcagggtt tacgccactg attcggctag cttgggcaat tgtttctggt
    11161 tggattttct gcaatttttg ttttgcttct gttgctaatc cgttgattgc tgcgtagtcg
    11221 atgttttctg gaatcttttt cgcttccatg cgttttaatt tttctacttt ttccatggct
    11281 tttttgatgt agccttcata tttgatttgg atttctactt gttcgatttc ttttcgtgtc
    11341 aattcttcat tttctggaat aaaccgtaga agttctgcat aagtaatctc aggacgtctt
    11401 aaaaagtcgc ttgctagtac accatctttt aatggtgccg cattaatcgt tgctaaaaag
    11461 tcttggactt cttctgttgg tttgattcgt acattcatca aacgtttgat ttcttgttca
    11521 actgcttctt ttttcttcaa ataattcttg tattgatctt cttttactaa gcctacacgg
    11581 tgacccattt ctgttaatcg caaatctgca ttatcatgac gtaaaagcag acggtattca
    11641 gcacgtgaag tcagcaaacg ataaggttcg ttcgttcctt ttgttaccaa atcatcgatc
    11701 atgacaccga tgtaaccgtc acttcgtttg atcactaaag gttcttttcc ctgaactttc
    11761 aaagctgcat tgataccggc aatcagccct tgtccagccg cttcttcata accgcttgta
    11821 ccatttgttt gtccagctgt ataaagattt tcaacgactt tagtttctaa tgtcggacgg
    11881 agctggtgtg gctctactac gtcatattcg atagcatatc ctgtacgcat catttctact
    11941 ttttctaatc cagcaataga atgaagcatg ttgacttgaa catcctctgg taaagaagta
    12001 gataaacctt gaacatagac ttcttccgta tttaagcctt ctggttccaa aaataattga
    12061 tgacgtggtt tgtctgcaaa acgcacgatc ttatcttcga tggatggaca atagcgagcg
    12121 cctacccctt caacgatccc tgtaaacatt ggggcacgat gtaagttgtc tcgaataatt
    12181 tggtgagtcg tctcacctgt ataggtcagc cagcaagatt cctgatctag tttataggca
    12241 ctatctggtg tgctgaagct aaaatgattc ggctctttgt caccaggctg ctcttctgta
    12301 acagaatagt cgatcgtact tgattttact cgaggcggtg ttcctgtttt gaaccgttcg
    12361 atagcaaagc caagttcttt tagatgattc gctagtccaa tagaaggttg agagttattc
    12421 ggacctgatg aatatttcaa ttcaccgata atgatttctc cgcgaagtgc tgttccggct
    12481 gtaataacaa ctgctttggc ccggtagctc gctcctgtgg acgtcactac tccatgacaa
    12541 acaccatctt cgacgatcag ttcttcgacg attccttgtc ttagcgtcaa atttggttct
    12601 ttttcgatcg tatgtttcat ttctgttgaa taagcatgtt tgtctgcttg tgctcgcaat
    12661 gctcggacag ctgggccttt tcctgtgttc aacatacgca tttggatata ggttttatcg
    12721 atattcttac ccatttctcc gcctaacgca tcgatttctc gaacgacaac gcctttagct
    12781 ggtccgccta cagaaggatt acatggcatg aatgctacca tgtccaaatt tattgtcagt
    12841 aataatgttt ttgatcccat acgtgcagca gcaagtgcag cttctgaacc ggcgtgtcct
    12901 gcgccaacaa cgatcacgtc atacgcatct gcttcataat gttccaatcg tttgttcctc
    12961 cttttatttt cctaaacaaa attgactgaa taattgagtg attagctcat cttgtacact
    13021 gtcgccgaca acttctccta gatagtccca gcaacgcgtc atatctattt gaacgagatc
    13081 cacaggcatt cctgaatcaa tacctgcaat gacttcttca agtgagaggg aagctttttc
    13141 taacaacgcg atatggcggg tattagaaac ataagtagcg tctctttctc ctgtttctcc
    13201 gctgaagaat aaatcagaaa tagctgattc caaacgatct agtccgtcgt ttttagctac
    13261 ggaaattgaa aaaactggtt cattttcgat caatttcttc aatttttctt gttccagctg
    13321 tgctggcaaa tctgttttat ttagaagaat gatccgtttc aatccacttg tcgcttccaa
    13381 taattgttca tcttctgctg tcagtggttc gctttggttt agaacaagta agatcaagtc
    13441 tgcttctgct aatgcttttc ggcttctttc gaccccgatc cgttccacca catcttctgt
    13501 ttcccgaatt ccagctgtat cgattagctt gagcggtacc ccccgaacat tgacatactc
    13561 ttcgattaca tctcgagtcg ttcctgcaat atccgtaacg attgcttttt cttctcttaa
    13621 caaatggttt aacaaacttg atttgccgac atttggccgg ccgatgattg ccgtacttaa
    13681 cccttcccgc agaaccttcc cttgcttgga tgtagcaagt agcgcttgga ttctttgttg
    13741 gatcatctgc gctttttcaa gcaataactt cgtagtcaat tcttcgacat catcatattc
    13801 tggataatca atatttactt ctacttgtgc taatgtctct aaaatctctt gtctcaatga
    13861 gcggatcaaa gcagacagat tgccgtctaa ctgattaaga gctagtccca ttgctttgtc
    13921 tgtttttgcc cgaatcaaat ccatcacagc ttctgcctgt gaaaggtcaa cacgaccatt
    13981 caagaatgct cgttttgtga attcgcctgg ctcagctaat cttgcacctt ctcttaacaa
    14041 tagctgcaaa atttgattga ctacaacgat cccaccgtga caattgattt ctactacatc
    14101 ttcccgcgta aatgtctttg gtgctctcat cacggagacc atgacttcat cgacaagctg
    14161 attgctcttg ggatcaacga tatgtccata atggatcgta tgacttggta cttctgataa
    14221 tcgtttgttt ccagattgat aaactttatc tgctagttgc acagcttgat cgccacttaa
    14281 acgaacgata ctgattgcac cttctccagg tggggtagaa attgccgcaa tcgtatcaaa
    14341 ttctaaagta atttcagcca ttttttcact tcctttttca ttttgcgcac aaaaaaagtg
    14401 cccactccac cctttatgca caataaacgg tgaacttcgc actttgactg cttgttcttg
    14461 attaaattct ggtcatagat tatcacactt gctataagga atcaagtgtt taaaacaatt
    14521 agatgaagtt gagcctgttc tgcttccatt accttatatt taattgtcct tggatgtctc
    14581 tttgcgttct ttggtttatt cgaagagaag atactgtata aacaaggctt tatctttctt
    14641 aaaaaaaacg gtacactaaa tggtagcgct atctaaacta tacacttttg aaaggagaaa
    14701 atatgtgagc ccgttcagat ctgctgcatt ggaagaagaa agtgaccctc ggatcaacga
    14761 tgtcgtttta tctacaggga atatcaacca gaaatatatc gtccgcgata tcgtttttgt
    14821 tgtggaatgc ttagaagctg atttgtttga cccatctttt cccaaagatg accttttctt
    14881 aacaacgaag aggaaattaa agacgaaggt acttgaatat ggtgcgaatg cagtaatcaa
    14941 ctgtcatttt gagtatcagc aaaaaaaacg cgacggaaag tattttctgg aactttttgc
    15001 ttatggaaca gtgatccaat ttatccaaac aacgattggt taaatgaaac agacaaaaaa
    15061 agcggatcaa aactgaagac agtttcgatc cgcttttttc tatcaaagtt agtgactact
    15121 tcatcgtata ctctgataag aatgatttct agtaaaatat acttggtttg aaaaactttg
    15181 tcaacgtgat ttattcaaaa agtaatggaa tcaataatat tttttagcag gttctactac
    15241 taaataacga taaggttcct caccttccga atgtgttttt acatgttcat ctttgcttaa
    15301 tgcagcatgg atttgtttac gttcaaatgc aggcattggt tctaagaaaa caggctgccc
    15361 tgtccgtttt actttttcag ccgtgcgatc agctaaacgc tgaatgatct cttggcgctt
    15421 ttcacggtaa ttgcctacat tgactactac agacagctta tttcttgcta aacgatggat
    15481 gaagacttgt gtcagatatt gcaatgcatt cagtgttttt ccatgtttcc caatcagtat
    15541 gccttgcttt tgtgtttcta aatggaaaac aaccatatta tttgattctc tttccatttt
    15601 gacaagagct ggagcatcaa gctgttttga aatttcagta agataaactg ccaattccgt
    15661 taaagctgct tcatcaccta gctcacttac ttcttctgtt ttttcttcaa gaatcgtttc
    15721 gttgactacc tcgatctcag ctgttggttc ttcttccaaa atttcttcta cagtctgttc
    15781 tactgtttca gaaacttttt cgctgatact tggctggatc gaaagccgtg cattctttcg
    15841 acccattcct aggaaacctt tttttccttc ttccagtatt tcgatttcga cttggtcttt
    15901 ggccaaaccc aaatcagcca gacctagttg gacagcttcg tctattgttg cagcttcata
    15961 aatcggcatt agcattacct cccctattat ttccgttttt tcttaggatt cattgctttc
    16021 ttaagagcac gttctctttc gcggattctc cgtgcttctt cttcacgctc ttttcgaatt
    16081 ttgaaagggt tgttgatcag cagcgtttgt acaacttgga aggcattgga aactacccag
    16141 tataatgaaa gaccgctggc taaattgaca cccatcacta aaatcattac aggcatcacg
    16201 aaattcatga tcttcattga tgcattcgat tcaatttgac tcatgctaga taaatacgta
    16261 cttgcaaaag taaagattgc tgctaaaatc ggcaaaataa atgttggatc cgcgttccct
    16321 aagttcaacc ataagaaatg cccttgtttc aactctggta cacgtgaaat agattgccat
    16381 aacgccatca agattggcat ttgtactaat aaaggaagac aaccagcata tggattgacg
    16441 ccattttctg catataaacg ctgctgctct tctcttaact tactttgtgt ttccggatct
    16501 cttgaactat attgttgctg cagtgctttt aatttcggtt gtaattcttg cgttttacgc
    16561 atgctctttg tttggaaatg catcaacggc aataggatca cgcggatgat cagtgtaaat
    16621 aaaatgatcc ctatccctgc attaccaaat gacaacgcct tgatcgcctc agcaaagtaa
    16681 taaacaatat aacgatccca aattcctgta ctctgtgcgc tgacttcccc agttccgcaa
    16741 ccggataaga cgatgaccaa actaacgata ccggccatca aaagtaaacg tttgtatttc
    16801 ttcacttact cttttcctct ctgtcgatta tttttgcaag ttttaagacg tggactaaat
    16861 tggagcgaac ctcatcataa gtcaacccct tcacacttgg tctagcaatc acaataaaat
    16921 ccaactccgg atcaatttct tcttttatag aataaactgc tgctcggatt tttcgtttga
    16981 cttcattgcg gtttaccgcg ttgccgacct tttttcctac agataaacca actcgaaaat
    17041 gaggctgtcc cggtttttcc aaacggtaaa cgacgaattt gcggttagca aaagaatctc
    17101 ccgtatggaa cacggcttga aactctcgtt ctttttttac tcgatacgct tttctcatta
    17161 gttttctttc cttttctatc tctcacttac gctcataatc ataccataat tacgattgtc
    17221 ctttcatcat ttccctaaaa aagagtataa aaaaaaccac tgaaacccca gtggcctaag
    17281 ctgaaagaac ttttcttcct ttgcgacgac ggctagctaa cacacgacgt ccatttttag
    17341 tactcatacg tttacggaat ccgtgaactt tttgacgttt acgtttattt ggttggtatg
    17401 ttcttttcat atattccacc tccaaaatgt tcttatctat tttataaaca cagacatact
    17461 ttgatagtat aacgacaaag tagtgatttt gtcaatataa aatagaagat cgattgttaa
    17521 aacttatcca caagattatt tttttctatg ggaatcctat gtggaaaaag ttatgcacag
    17581 ggattatcca cagataataa aaggctggtt ttacacagaa aacacacgtg taaaaaaagt
    17641 aatccacaat caggcgattt cctgtgtatt ccgtctccta actattggta tatctgtatt
    17701 ttttatccac agacgcaaga ggaaatctat tggtttttac gagttttcca cctgtgcatt
    17761 agtggtgtgg aaaacttttt ttgacaactg tgtttttctc ttttttcttt ttgtcgattg
    17821 tgtggataat tgtaattaaa aaattttttt cttcggtttc tgtggaaaac tattgaaaaa
    17881 aatgctaaat tagtactagt tattcacttt ttaatgaagg aggaggcctt atatggtatc
    17941 cctcgatgct ttatggaatg aattaaaagc aacataccaa aaggatttat cgcctgctag
    18001 ctataataca tggatcgaaa cagcgaaccc tcgaaccctt gatcaaaacc agctcgttgt
    18061 tgaggttcct agcaagattc ataaagaata ttgggaaaaa aacctggcga caaaaatcgt
    18121 tgaagtcggt tatatgttat caggaaatga gattatccct cgctttatta ctggcgaaga
    18181 agcagagcaa gaagaagtta tagaagaaaa aaatccaaaa gttgtggcac ctagtccgct
    18241 aaaaaaagcc atgctgaacc ctaaatatac ctttgatacg tttgtcattg gtaaaggaaa
    18301 ccagatggct catgcagcag cgctcgttgt agcagaagat ccaggttcta tttataaccc
    18361 gctcttcttt tatggaggcg tgggacttgg taagactcac ttgatgcatg caattggtca
    18421 tcaaatgctg caaagtcagc ctaatgccaa agtaaaatat gtaagcagtg aaacttttgc
    18481 taatgatttc atcaattcga tccaaaacaa aacagcagaa gagttccgtc aagaatatcg
    18541 aaatgttgat ttactgctag tggatgacat tcaatttttt gcagaaaaag aagctacgca
    18601 agaagagttt ttccatactt tcaatgcctt gtacaatgaa gggaaacaaa tcgtactaac
    18661 cagtgatcgg ttgccaaacg agattccgaa actgcaagaa cgccttgttt ctcgctttgc
    18721 atgggggctg tctgttgata ttacaccgcc ggatcttgaa acaagaacag cgattttacg
    18781 taaaaaagcg gcggctgaac gtctagagat cccagatgac acactaagct acatcgcagg
    18841 acaaattgat tctaacattc gtgaattaga aggtgcgctt gttcgagtgc aagcttttgc
    18901 tgcgatgaat agtgaagata tttcgactag tctagctgca gacgcactga aaacgttaaa
    18961 atcaggaaaa ggacatcctc aattgtcaat cctgcagatc caagaagaag tggcaaagta
    19021 ttatcacatc caattgaaag atctcaaagg aaagaaacgc gtcaaatcta ttgttgttcc
    19081 tagacaaatc gctatgtatt tggcacgtga gctcacagat aattctttac cgaaaatcgg
    19141 agctgaattc ggtggcaagg atcatacaac ggttatccat gctcatgaaa aaatccagca
    19201 gctgatggat tctagtgtca gtatgcaaaa tgaagtatca gaaatcaaaa actttttact
    19261 aaattaattt gtcgattcta tcaactgaaa atagcatgtg gataaaagaa aaaaacgttc
    19321 aaaagttata cacacttttt ccacagctgt tttttcgttt ctctcttacg ttttttgagt
    19381 tttccacaga atcaacatgc cctattactt ttattattct ttttaactaa taataaataa
    19441 acaaacattc aaaggagata catcatgaaa gttactttaa accgagctag ctttatgcag
    19501 gaattgcaaa ctgttcaacg agctatttca agcaaaacca cgatccctat tttgacaggt
    19561 gtaaaaatca cactgacaca agaaggtttg actttgacgg ggagcaacgc tgatatatca
    19621 attgaaactt ttttgtctgt tgaaaacgaa aaagcaaata tgcaaatcga atctactggt
    19681 tccattgttt tacaagcacg tttctttagc gaaatcattc ggagacttcc tgaagaaaca
    19741 tttactttag aagttttaga aaataaacaa gtagcgatca cttctggaaa agcgaatttt
    19801 atcgtaaatg gattagatgc agataactat cctcatcttc ctgttgtcga aagccataac
    19861 cagatgaaat tacctgtaca cgtattgact aaactaatca acgaaacagt ttttgctgtc
    19921 tcacaacatg agagtcgtcc aatcttgaca ggtgtgcatt tcattttatc tgataattct
    19981 ttattagctg tagctactga ttctcaccgt ctaagtcaac gcgtgattcc agtagaacaa
    20041 gcggctgatc attttgatat tgttattcct ggaaaaagtt tgatcgaatt atctcgctca
    20101 ttaacaaatg aagaagaaat cgtcgaaatc agcattatgg aaaaccaagt gttgttcaaa
    20161 acagaaacga tgtatttcta ttcccgtttg ctagaaggaa actatccaga taccaatcgt
    20221 ttgattcctt caagttttaa tacagaggtt gaattttctg ttcctagctt tttagcggcg
    20281 atcgaacggg cttctttact ttctcatgaa ggccgtaaca acatcgttcg tttatctatt
    20341 cgtccagatg cggttgtttt atacggaaat tcgccagaaa tcgggaaagt agaagaaagc
    20401 ttgagttata cagcaagcag tggcgatcca ttagatattt ctttcaatcc ggattatatg
    20461 aaagcagcgt tgcgtgcatt tggagatatg agcatcaaag tgaaatttat ctctgctatt
    20521 cgtccattta cattggaacc aacggaagat ggcgttcaat ttattcagct gattactcct
    20581 gtacgtacaa attaacaatg attttcaatg ttcttgaaaa tttaattgaa aagaaataga
    20641 aaaaataaaa ttttaaacag aaggccctaa aatctctttt aaggctttct gtttattttt
    20701 gtttaaatca ccttttttag attaaatcgt cttatatgga aaaatatatg gataaatttg
    20761 ttttattgcg aaaaaaaagg tataatagag tatactttta ataatgaaaa tgagggatgg
    20821 gcattgaaac aacaagtcat tctaaatacg gaattcatga cgttgggcca aatgttgaaa
    20881 gaagtcacag ttatcggtag tggcggacaa gcaaaatggt atttggcaga aaacactgtg
    20941 ttagtcgatg gcgaaccaga aaatcgtcgt ggacgcaaac tttatcccgg catgatggtt
    21001 gaagttccag aagtaggaac tttttttatg gtaaaaaaag gaaacgacga taatgaggct
    21061 gaataagctg tatttaaaaa attatcgaaa ttacgaagag ctgaacatcg aattttccaa
    21121 gaacctcgtc atctttttag gagaaaatgc gcaaggaaaa acgaatcttt tggaaagtat
    21181 ctacgtatta gctatgacgc gaagtcatcg gacaagtagt gagcaggaat tgattggctg
    21241 gactgacgat caggcgatga ttcaaggaga gatcacaaaa ggttcatcca ctcttccatt
    21301 agaaatctta ttatctaaaa aaggccgaaa aaccaagatc aaccatatcg aacagaaaaa
    21361 gttaagtact tacgtgggac aaatgaatgt cattttattt gcaccagaag atctttcttt
    21421 agtaaaaggc agcccgcaga tcaggcggaa gtttttagat atggaattag gccagatcaa
    21481 tccaatctat ctttatgatc ttgtccaata ccaagcaatt ttgaaacaaa gaaaccagta
    21541 cttgaagcaa ttgaatgaaa aaaaacaaac tgatttgctt tatttggata ttttgtccga
    21601 acaactagca gcgtttggca gcaaagtatt gaaagcaaga gcacagttcg tcaagcgatt
    21661 ggagttttgg gctaattctt tgcatcagca aatcacccat caaaaagagc agctggaaat
    21721 cgagtatctc accgcagtcg attctttaga aacacatacg caagagcaaa tccaagaaca
    21781 atttctcgca cttttgaacc aaaataaaaa gaaagacctt tttcggggaa caacgacagt
    21841 tggtcctcat cgtgacgatc tttccttttt tattaatcag aaaaatgtgc agacttatgg
    21901 atcgcaagga cagcaaagaa caactgcact aagtgtcaaa ttagcagaaa tcgatttgat
    21961 taaagaagag acaggggaat atccaatcct tttgttagat gatgtgatga gcgagctgga
    22021 tgataatcgt caactccatc tgctagaaac aatcgaagga aaagttcaaa cgttcttaac
    22081 aacaacgaca cttgaccatg taaaaaataa aatgactgtt gaacctgaga ttttttatgt
    22141 tcaacaaggg aaactaaaag gagaagaaac tgaatgacag aagaaagaag tttagtagaa
    22201 cgcgctaaag agtatgatgc cagtcagatt caagtacttg aaggacttga agccgttcgt
    22261 aaacgacctg gcatgtatat cggttcgact agttctgagg gtctgcatca ccttgtttgg
    22321 gaaattgtcg ataattcgat cgatgaagta ttagcaggtt ttgcaacgaa aatccatgtg
    22381 attattgaaa aagacaacag tatcaccgtt attgacgacg gtcgggggat acctgtagat
    22441 atccaggcaa aaactggacg tcctgcagtt gaaacggtct ttactgtttt acatgctgga
    22501 ggaaaattcg gcggtggtgg gtacaaagtt tctggtggac ttcacggagt aggatcttct
    22561 gttgtaaacg cgctttctac acagcttgat gtcaaagtat acaaagaagg aaatgtgtac
    22621 tatcaagaat atcgtcgtgg agctgtagtg gacgacttaa agattatcga acaaacggat
    22681 cgccatggaa caacggttca ctttacgcca gaccctgaaa tttttacaga gacaactgag
    22741 tttgattttt caaaattggc tacccggatc cgtgaacttg ctttcttgaa ccgcgggatg
    22801 cgtatatcga ttgaagacaa acgagaagaa gaaccagtca tcaatgaata ccattatgac
    22861 ggcgggatca aaagttatgt cgaatacttg aatgcaaaca aaaccgtgat ttttccagaa
    22921 cctgtatatt tggaaggtga acagcaagat atcgcagttg aagtctctat gcaatataca
    22981 gacggctacc attccaatat catgagtttt gccaacaata tccatacgta tgaaggtgga
    23041 acacatgaat caggattcaa gactgcttta actcgtgtga taaatgatta tgcccgaaaa
    23101 caaaagctga tgaaagaaaa tgacgacaac ttgaccggtg aagacgtgcg agaaggattg
    23161 acagctgtta tttctatcaa gcatcctgac cctcaattcg aaggacaaac aaaaacaaaa
    23221 ttgggcaatt ccgaagtacg gacagtcact gatcggttat ttagcgaaca tttcatgaaa
    23281 ttcttgctgg aaaacccaag tgtgggccgg caaattgttg aaaaaggatt actagcttcc
    23341 agagctcgtc tagcagcgaa acgtgcgcgt gaagtaacga gacgtaaagg tgctttagag
    23401 attagtaatc tgccaggaaa attagcggac tgctctagca acgatcctga aaaatgcgaa
    23461 ttgttcatcg tcgaaggaga ttcggccggc ggatcagcta agcaaggtcg taatcgagag
    23521 tttcaagcca tcttgcctat tcgtgggaaa atcttgaatg ttgaaaaagc aagcatggac
    23581 aagatcttgg caaatgaaga gatccggtct ttatttactg ctatgggtac agggtttgga
    23641 gaagattttg atgtatcgaa agctcgctac cacaaattag ttatcatgac tgatgccgat
    23701 gtcgacggcg cgcatatccg tacattgctg ctgactcttt tttatcgtta catgcgtcct
    23761 attgttgaag ctggatacgt ctatattgct cagccacctt tatacggggt aaaacaaggg
    23821 aagaatatta cgtatgtaca accaggaaaa aatgcagaag aagagttgaa acaagtcgtt
    23881 gcttcacttc ctgcctcacc aaaaccaagc gttcagcgtt ataaaggact tggagaaatg
    23941 gatgatcatc aactatggga aacaactatg gacccaagca atcggatgat ggcccgcgtc
    24001 agtgtggacg atgctattgc agcagatcag atatttgaaa tgttgatggg ggatcgagta
    24061 gaaccccgtc gtgcattcat cgaggaaaat gcacactatg tgaagaactt agatatttaa
    24121 tcatgaagga ggaaaaagaa tgagtgaaga aatcagagaa aacatccatg atgtcaatct
    24181 aaccagcgaa atgaaagact ccttcatcga ttatgcgatg agtgttattg tcgcacgtgc
    24241 acttcctgat gtcagagatg gtctgaaacc cgttcatcgc cgtattttat acggcatgaa
    24301 cgaattaggt gtaacaccgg ataaagccca taaaaaatct gccagaatcg tcggagatgt
    24361 catgggtaaa taccatcctc atggagacat tgcgatctat gagtcaatgg tacggatggc
    24421 acagccattc agctaccgtt atatgctagt ggatggtcat ggaaactttg gttctgttga
    24481 cggtgacggt gccgccgcta tgcgttatac cgaagcacgg atgagcaaaa tcgctacaga
    24541 aatgctgcgt gatatcaata agaacactgt tgatttccaa agcaactatg atgatacaga
    24601 aaaagaacca gttgtactac ctgctcgctt cccgaatctt ttagtgaacg gaacaacggg
    24661 gatcgccgta ggtatggcca cgaatatccc acctcataac ttgtctgagg tagtagcggc
    24721 tatcgatttg ctgatggata atcctgatgt gacgaccaat gaattgatgg aagtattacc
    24781 tggaccagat tttccaacag gtggtttggt tatggggaaa tcaggaatcc gaagagcgta
    24841 tgaaacaggt aagggttcta ttaccgtacg tgcaaaagtt gagattactg agatgccaaa
    24901 tggtaaagaa cggatcatcg tgacagaatt gccttatatg gtcaataaag caaaattgat
    24961 cgaacggatt tctgaactgc acagagacaa gcgtatcgaa gggatcacag atttgcggga
    25021 cgaatcttcc cgtgaaggta tgcggatcgt cattgacatc cgtcgtgacg ccagcgcatc
    25081 cgttatcttg aataacttat ataaattgac atccttgcaa aattcatttg gattcaatat
    25141 gttggcgatc gaaaaaggtg tgccgaaagt tttgagcttg aaacaaatcc ttgaaaacta
    25201 tatcgaacat caacgcgaag tcatcacacg acggacggtc tttgaaaaag aaaaagcaga
    25261 agcaagagca cacatcttag aaggtttgcg tattgctttg gatcatatcg atgaaatcat
    25321 tgcgattatc cgcgggtcta aatcagatga cgaggcaaaa gcaacaatga tcgaacggtt
    25381 cgatttatcc gatcgccagt ctcaagctat tttagatatg cgtttacgcc gattgaccgg
    25441 tttggaacgg gaaaaaatcg aaaatgaata ccaagaactc ttaaaactga ttgaagattt
    25501 aactgacatt ctggcaagac cagaacgtgt tacagaaatc atcaagaatg aattagcaga
    25561 cttgaatcaa cgatttggtg ataaacgacg cacagaatta ctagtcggtg aggtcttaag
    25621 tctggaagat gaagatttaa tcgaagaaga agaaatcgtt attacattga caaacaatgg
    25681 ttacatcaaa cgtttggcaa ataatgaatt cagagctcaa cgaaggggtg gacgtggtgt
    25741 ccaaggaatg ggtatccaca atgacgactt cgtcaaaaat ctggtttctt gttctaccca
    25801 tgatacattg ttattcttta cgaataacgg aaaagtgtat cgggcaaaag gatacgaaat
    25861 tccagaatac gggcgtacag ccaaaggaat cccaatcatc aatttactag gtatcgattc
    25921 gagtgaaagc atccaggcaa tcattgcggt gacgggagaa gcagaagaag gccactatct
    25981 cttctttacg acgcgtcaag gtacagtaaa acgtacttct gtcactgctt ttgctaacat
    26041 ccgaagtaat ggtctgatcg caattggttt gaaagaagac gatgagctcg tcaatgtgct
    26101 actgacagat ggtcaatcta atatcattat tggaacacat aacggctact ctgtcacttt
    26161 caaagaagaa gcagtccgcg atatgggacg tacagcttcc ggggtcagag ggatacgcct
    26221 gcgtgaggaa gattatgtcg ttggggcagc cttaatgaag gaaaatcaag aagtattagt
    26281 gatcacagaa aaaggttacg gaaaacggac aaaagtatcg gaatatccag tgaaaggccg
    26341 cggaggtaaa gggatcaaaa cggcgaacat cactgaaaaa aatggtccac tagctggatt
    26401 gaccactgtt tctggagatg aagacatcat gctgattaca gataaaggtg tcattatccg
    26461 tttcaacgta tcgactgttt ctcaaactgg acgttcaaca ttaggcgtac gtttgatgaa
    26521 gatggaagct gatacgaaag ttgtcacgat ggcagcagtc gaaccagaag ctgcagaaca
    26581 aacagttgaa gaacaaggat cagaagaata accatcaaga ctaagtgtgt aagctagcaa
    26641 gaataaccga ttaaaaaatc tggttattcc tgctggcttt tttgattagt tgaatttctc
    26701 ttaaaatatt cgaaactgtt cattctgtat tgcttttgac caaaatagag tgtataatta
    26761 gagcttgtga gtagtctaat taattagata actctccttg ctcttaggaa cctaagagcc
    26821 gaaagtccac agggaggtga atatcaatgg aaaacacgaa atatgaaatc atgtacatta
    26881 ttcgtccaaa cattgatgaa gaagcaaaaa ctgctttaat cgaacgtttc gatgcaatct
    26941 tgaaagataa cggagcagaa gttatcgaat caaaagattg ggaaaaacgc cgtttcgctt
    27001 atgaaatgaa cggttaccgt gaaggtatct atcacatcgt taaagtatct tctccatcat
    27061 cagcaaatgc tgtcaatgaa tttgaccgtc ttgctaaaat caacgacgat atcattcgtc
    27121 acatgattgt taaagaagaa gcttaattgt tgtttcatgt gaaacaatag aaaacgatga
    27181 gaggagatga ctattttgat taataatgtt gtattagttg gacgtctgac gaaagatcca
    27241 gatttgcgtt acacagcaag tggtacggca gtcgcaacat ttactttggc tgtgaatcgt
    27301 aattttacaa accaaagcgg caatcgcgag gctgatttta tcaactgtgt gatttggcgc
    27361 aaatcagcag aaactttagc aaactatgct cgtaaaggaa cacttttagg tgttactgga
    27421 cgtatccaaa ctcgttctta tgataatcag caaggacaac gtgtatacgt gacagaagta
    27481 gtagctgaca acttccaatt gctggaaagt cgttctgcat ctgaaaatcg ccaacaaagc
    27541 ggcggattcc aaagttctgg tcaatcagct ggcggatttg gcggaaacaa taactctaat
    27601 caaacatctc aatcatccaa cggtatgcct gatttcgacc gtgatacttc tgacccattc
    27661 ggctcaagtt caacgatcga catttcagac gatgatttac cattctaaac gaatttgata
    27721 ggagggaaat agaatggcac aacaaagaag aggcggacgt aaacgtcgta aagtagacta
    27781 cattgctgct aaccatattg aatatatcga ttataaagat atcgaattac taaaaagatt
    27841 tatttcagaa cgcggtaaaa tcttgccacg tcgtgtaact ggtacaggcg ctaaaaacca
    27901 acgtaaaatt actgttgcaa tcaaacgcgc tcgtatcatg ggcttgctac ctttcgttag
    27961 cgaagactaa taaatcctag gaattgaatg actgatcctt ttgatcagct attcaattcc
    28021 tttttttctg taaaaataac ctctattcgt ttagtcgctc gaatgacgaa agttatggta
    28081 aaatcaaatt agtcatatat gggaggaaaa ttatggaaaa taaaaacaat catttatccc
    28141 cgaaacctct cttccttatc ctgtttatat taatcgaagt attggtagtc ttaattttac
    28201 catttcgttt tgttagcgct tcggtcattc tattgatgaa tttatggggt gttcgctatt
    28261 ggttgaaaat gaaacgttgg cttattttaa ataaacgtga aaaaatccag caggcttcga
    28321 ctgtagctga aaccaatcta gcttatgtct cagaagtcat gcctgtagga gtgattgctt
    28381 atcagccatc aagccaaaaa atcgaatggc tcaatccatt agcattgaaa atcaaagatc
    28441 aaagtggatt aacagaggaa gaattgctag atactttttt tactgcaaaa gaaaaaaaga
    28501 aaaaccaagt ctatctagca gataaaacat ttcagtttga tatggataca gtaaagggtg
    28561 ttgtttattt taatgatatt acccaaaacc agttgttaaa actccatcag acgaacagtc
    28621 agccggtgat tggtatttta tctattgata actatgcaga tgccattgat aaattagatg
    28681 acaaagagat ttcttatttg aactctttcg tcactacact agtttctgac tggatgaatg
    28741 aataccatgt ctttttcaaa agaatcaacg cagaacggtt tttcttcgta gcacagatga
    28801 tcgatctgaa aaagatgatg gataaaaaat ttgatttgct ggatcgcttt cgagcagaag
    28861 ctgaaaaaca gagctcgcct atcaccctta gtatggggat tgcttatggc tcaagaaccc
    28921 tcgatgaaat cggtggagaa gctcagaata atttagatgt tgctttagtc cgtggaggcg
    28981 accaagttgt cgtgaaagag gtaaaagaag gagcaaaacc tgtttattat ggggggaaat
    29041 ctgctaagaa tgtcaaacgc acgcgtgttc gttctcgagc gatgagtacc gctttgcgtc
    29101 ggatttttga tgaaacaggc gatgtgttta tcatggggca caagtttccg gatatggatg
    29161 ctttaggctc tgcttttggt gtcagttgtc tggcacgctt caaccagaaa aaagcttaca
    29221 tcgttatcaa tgagaacgag atcattccag atgtagaacg gtgtcttgaa gaaatccata
    29281 agcatcctga attagaaatg caactaatct ctccagaacg tgccatagag ctgaaaaaag
    29341 aagaggacct cctcgtgatg gtggattacc ataaaccttc tctatcgatc tctcagggcc
    29401 tatatgagca atttgataag attgtgatca ttgatcacca tcgtcgtggg gaagagtttc
    29461 caagtaaacc tctgctgact tatattgaat cttcagcttc ttcagcttca gagctagttt
    29521 ctgagctgat ccaataccaa agcagcaaaa caaatcgctt agatcgtttt gaggccacat
    29581 tattgcttgc agggatggtt gtagatacaa atagtttcaa tgtccgtaca actgcacgaa
    29641 cttttgacgt tgccagctat ttaaggtctt gtggagcaga cgcatcctta gtacaatatt
    29701 tattgagctc ggatttagcg acatacttgg agatcagcaa tttgattgct ggaagcgact
    29761 atgtgacttc tgatattgtt gtggcggcag ggacagacga aaaagagtat aatagtgtta
    29821 tagcagcaaa aacagcagat acattgcttt cgatgatcaa tatcaatgca gctttcgtca
    29881 ttacaaagcg gacagacggc ttgattggga tcagtgccag aagtaccggc acgatcaacg
    29941 tccaaatcat catggaatcc ttaggtggcg gaggacactt cacaaacgca gctactcaac
    30001 tggaaaatac gacagttgag gaaacaaaac agaagctatt tgctgcaatc aaagaaaata
    30061 tgaatcaaat atatgataag gagtaatcca aatgaaagta atttttttac aagatgtaaa
    30121 aggaaaaggt aaaaaagggg acatcaaaga agttccagca ggttatgcac aaaattatct
    30181 aatcaaaaat aacttggcaa aagaagcaac gaaaagcagt atcagtcagt tagcaggaca
    30241 aaagaaagca caagaaaaac acgaagctga aatcttggaa gaagctaaaa agttaaaaga
    30301 attcttggaa aaagaagata ctgtagtcga attgaaggcg aaagcagggg aagacagccg
    30361 tttgtttggt tcgatccctt caaaacaaat tgctgaaggg ttaaacaaac aatacaaaat
    30421 caaattagat aaacgaaaaa tcgaattaga aaatccgatc cgttcactag gctacacaaa
    30481 cgtacctgtt aagcttcatc atgaggtgac ggctacgatc aaagttcatg tcgtacaaga
    30541 ataacaaatc ttgatagcga tgttcagctg gtaagtataa ccagaaggaa aaaagacaaa
    30601 ccgaaattta tctgagaaaa cagctgcagc aatgaaagca aaagctgcaa atcgatttca
    30661 gattgatttc ggtttctttt ttgggaacag caaaatagca taaagaaaac gagtataata
    30721 aaagagaact atttttttat tacactttcg gtacgaatga tgacttttct ctcattttct
    30781 tgtaaaatga agcaattgaa gacaaggagc gatacgtatg aatgaagtgt ggcaggatcg
    30841 aatcccaccg caaaatattg aagctgaaca agcggtttta ggctctgttt ttcttgacgc
    30901 agaaacgatc attgatgcca tggagtacat agagccaaaa gacttttatc gtcgtggcca
    30961 tcaaattatt tttgagacaa tgatcgagtt aaatgatcgt aatgaagcga ttgacgtggt
    31021 tactgtcaaa gaccggctgg aacaagccaa tttgttagaa gatgccggtg gtttaagtta
    31081 tctctcagac ttggcattag cagttccgac agcagcaaat atcgtctact atgcgaaaat
    31141 cgttgaagaa aaatcattgc tgcgtacatt gatccagaca gcaacaggaa tcgttacgaa
    31201 aggattcgag caaggagaag aagttcaaac aattttagat gatgccgaac gcagtatctt
    31261 ggaggtttcg gaaaaaagaa atcgaagcgg ctttttatct attgctgaag tactgaactc
    31321 cacgattgaa aatatcgatc gactagctca aaacaatgaa gaaatcacag gtctaccaac
    31381 tggctatcaa ggtctggata aaatgacagc aggtttccag cccgaagaac taatcattct
    31441 tgctgcacgt ccagcggtag gtaaaacagc gtttgcattg aatattgctc aaaatgtagg
    31501 aacaaaaaca gatcgttctg ttgcgatatt tagtttggaa atgggggcag aatctcttgt
    31561 aaatcggatg ctttgtgcag aaggttcgat tgaggccagc catttacgta caggtcagct
    31621 ttcggaagaa gaatggtcga atctgatcgt tgcgatgggg agtctttctc gagcaaatat
    31681 ttttatcgat gatacaccag ggatcaaaat ctctgaaatc cgtgcgaaat gtcggaaact
    31741 agcgcaagaa aaaggcaatc taggcttgat attgatcgat tatttgcagt tgattgaagg
    31801 taacggtaaa gagaaccgtc aacaagaagt atcagatatc tctcgccaac tgaaaaaact
    31861 ggcgaaagag ctgaaagtac ctgtcattgc tctttcacag ttatctcgtg gagtagaaca
    31921 gcgacaagac aaacgaccag tgctaagtga tattcgtgaa tccggatcaa ttgaacaaga
    31981 tgcggacatc gtagccttct tgtatcgaga tgattattat gaaagagaag gcggagatga
    32041 cgatgatgga aacgagccaa aaaatgacaa cgtcatcgaa gtcatcatcg agaagaaccg
    32101 aagcggtgca cgaggaacag ttgaactact gtttatcaaa gaatacaata agttttcgtc
    32161 gctttcgcca agagaagaat tttaaagaag aggttgtgag ccttgagcaa tcccgagagc
    32221 tctcgtaaga agatgcaagg agtaaccgca ggaacgacta gagtacttaa tttaaacaga
    32281 tctccaaagg gattgtgaca aaagtcttgt cacagtccct ttttccgaat aaacggtgtt
    32341 caatcagctg acccttggta ttagctcagc aaacgtcaaa atatgaggaa ctattttgac
    32401 gtttgtttct ctaatactca ggtcaaaacg ctgatcttac gacctctttt ccttaaacgg
    32461 tgttctcctg ccaactcctc ggtcacaagt cacaatatca tcaaacatga gaagcagttt
    32521 tttgaaattg ctccttgtga ctcagagtta aacggcaggc tcacaacctc tttcttaaac
    32581 ggtgttcaaa agcttgcatc tgcggtaata cgcccaatca aacgaaaaat atgagaagct
    32641 atttttgttt ggctgttctt attacttgat gcaggacagc tttttcacaa cctctgattc
    32701 acggtgttcc aaaagtagct tcttcgaaaa ataagtcgaa atttcacaaa aatttgagaa
    32761 gcaattttcg tacattcctt cttatttctt gaagctgacc acttctgtta caacctcttt
    32821 tttaataacc attctaaaaa gataaagttc gttttttaag cgtttttata aaataaaaat
    32881 aaaaatcatt cgattttatt tgaaatatgt atctattatt gatacaatgg ttcaggatta
    32941 aataatatag aacgaggtgt tcacatgtca tctgtagtag tagtaggtac gcaatggggc
    33001 gatgaaggga aaggaaaaat cacagacttt ctcagtgaaa atgctgaagt gattgcacgg
    33061 taccaaggtg gcgacaatgc agggcacaca attaagttta acggtgtgac ttataaactg
    33121 catttgattc cttctgggat tttttataaa gacaagatca gtgtgatcgg aaacggcgtt
    33181 gtagtcaatc ctaaatcatt ggttaaagaa ttagcttatt tacatgaaaa aggtgtagaa
    33241 acaagtaatt tgcgtatttc tgaccgcgcg cacgtaatct tgccttatca tatcaaattg
    33301 gaccaattac aagaagatgc taagggcgaa aataagattg gtacaacgat taaaggaatc
    33361 ggacctgcct atatggataa agcagctcgt gtaggtatcc gtattgcaga cttattagat
    33421 aaagaaattt ttgaagaacg tcttcgtttg aatctagatg acaaaaaccg tcaatttgta
    33481 aaaatgtttg attcagaacc acttgatttt gacgatattt ttgaagaata ttatgaatac
    33541 ggacaacaaa tcaaacaata cgtcacagat acttccgtta tcttgaatga tgctttggat
    33601 gcaggcaaac gcgtgttgtt tgaaggcgca caaggtgtaa tgctggatat cgaccaggga
    33661 acttatcctt ttgttacttc ttctaaccca gtagccggtg gggtaacaat cggaagcggt
    33721 gtagggcctt caaaaatcaa taaagttgtc ggcgtctgca aagcctatac gtcacgtgtc
    33781 ggtgatggac catttccaac tgaattattt gatgaaacag gagaacaaat ccgcgaagta
    33841 ggacgagaat acggtacgac aactggtcgt ccgcgtcgtg taggctggtt tgattctgta
    33901 gttatgcgcc attctaaacg agtttcaggt attaccaacc tatctttaaa ctcgatcgat
    33961 gtattaagtg gattggaaac agtaaaaatt tgtacggctt atgaattaga tggcgaacta
    34021 atctaccact atccagcaag cttgaaagag ttgaaccgct gcaaaccaat ctatgaagaa
    34081 ttgccaggct ggtcagaaga cattacagga tgcaaaacac ttgcagaact gccagaaaac
    34141 gcacgtaact atgtacgtcg tatctcagag cttgttggtg tacgtatctc aacattttct
    34201 gtcggacctg atagaacaca aacaaacatc ttagaaagcg tctgggcgca aatctaagga
    34261 taaaaacgac gactgaatca ggacgttctt accagagcga gtcgaagtgt ttatctatac
    34321 gataataata acttgaagag gtagtgaaag agacgtttag ccttattgcc gataggctgt
    34381 ctcttgaaca ctgtttattc agaataaggg actgtgacaa aaaccgtgtc acagtccctt
    34441 tttccgaaca aacggtgttc aaaagcttgc atctacggca ctaagcccaa tcagatgaaa
    34501 aatatggagt gctattttta tctgattgtt cttactgctt gatgcagagc agctttttca
    34561 caacctctcc actaaacggt gttctcctgc caactcctcg gtcacaagtc acaatattca
    34621 acaaacatga gaagctgttt tttgaaattg ttccttgtga ctcggagcta aacggcaggc
    34681 tcacaacctc ttggtaaacg gtgttcaatc agctgaccct cggtattagc tcaaaaaacg
    34741 gcaaaatatg aggaactatt ttttccgttt ttttctctaa tactcaggtc aaaacgctga
    34801 tttcacaacc tctgattcac ggtgttccag aagtagcttc tacggaaata agccgaaatt
    34861 tcacaaaaat ttgagaagca attttcgtaa attccttctt atttcttgaa gctgaccact
    34921 tctgtcacaa cctcttattt tgattagaac ttgcgaaaat aaaccggtta aagtgtgtct
    34981 agcgcaagag atttgctata atagacgata agggtaaaat gtaacgagaa ggggttgaaa
    35041 aatgtgtatc aaattgtaac agattcttgt tgtgatctgc cataccatgt attagcaaaa
    35101 gaaaaagttg attttttatc aatgaatctc caattgaatg atcaagagtt agtagatgac
    35161 ctcggaaaga cattcaatta tgaccgctac atgaatcagc tgaaaaatgg tgcgatgcct
    35221 acgacatcac aaatcaatgt tggccgatat ttagaatttt tccgttctta tgcggaaaaa
    35281 cagattcctg ttttatatgt ggctttttcc agtggattaa gcggctctta cagtagtgcg
    35341 cttcaagcgg ttgaattgct gaaggaagaa tatgaagacc ctgagatcta tgtttttgat
    35401 acaaaagctg caagtattgg ggaagggctt ttagtattgg aagctgtaag attgaaaaaa
    35461 gaaggtaaaa gcatcgaaga aaccctagag tggctggaaa gtaattattt gaaagtccat
    35521 tcatgggtaa cagtagacga tctgaaatat ctagaacgag gcggtaggat ttccaaagca
    35581 agtgctactc ttggcagtct attgagtgta aagcctatta ttacggtgga taaagaagga
    35641 aggctgacga atgttgacaa agttcgagga agaaacaagt cgatccagac aatcgtcaat
    35701 gagacggtaa aaacaatcgt tgcaccactt gatcaaatcg tttatgtggc ttacgctggg
    35761 gatctagaga gcgcggagaa ggcaaaacgg cttttggaag agcagataaa gatgaaagat
    35821 gtgaagcttt atcccttagg accaacgatc gccagtcaca caggctatgg ctgtatcgcg
    35881 attttttcga tgggaatcag tcgataaaag cagtaactca ttctgtaaaa aagcccagaa
    35941 attgattgta acaaaaacag cgactcttaa aagatagatg agcatcctat ctcttagagg
    36001 tcgctgtttt tttatttttt aagctaaata ggccattatg cccataactc gttcaattgt
    36061 ttttgaatat ctgcattttc caaaaattca tcatatgtcg tatcagctcg atcgatcaca
    36121 cctttatccg aaacggcaat gatgcggttt gccaatgttt ggataaattg atggtcatgt
    36181 gacgcaaata agattgagcc agtaaatgcc atcaagccgt cattcaatgc tgtgattgat
    36241 tctaagtcta aatggttcgt tggatcatct aatacaagta cgttagcttt cgaaagcatc
    36301 atttttgaaa gcatgcaacg gactttctct ccaccagata aaacattcac tggtttcaat
    36361 acttcttcac cagaaaacaa cattcttcct aagaagcttc ttaagaaggt gttatcatct
    36421 tcttctttac ttgcatattg acgcaaccag tctaaaatcg ttagatcagt atcgaattct
    36481 ttcgttgtat cctttggtaa ataagaatta cttgttgtca caccccaacg tactgttcca
    36541 gtatctggcg taatttcacc catgataact ttgaatagcg ttgttgttgt tatatcattt
    36601 tctgcgacga aagcgacctt gtcttctttg ctcaaattaa aagatatatc atctaaaatt
    36661 ttctttccat caatcgttac acttacattt tctacctgca ataagtcgtt tccaatctca
    36721 cgttcaggtt tgaatccaac gaatggataa cgtctagaag aaggttggat atcgtctaaa
    36781 gtaatttttt caagcatttt tttacgtgaa gttgcttgtt ttgattttga tgcattcgca
    36841 ctaaagcggg caataaattc ttggagttct ttgatttgtt cttctttttt ggcatttgcg
    36901 tttgcttgaa gtttagccgc tagttgacta gattccaacc aaaaatcata atttcctaca
    36961 taaagtttga ttttaccaaa atctagatcc gccatatgtg tacatacttt gttcaaaaag
    37021 tgacggtcat gggatacaac gatcacagta ttttcaaaat tgatcaaaaa ttcttccagc
    37081 caattgatcg attggatatc cagaccgttt gttggttcgt ctagcaaaag aacatcaggt
    37141 ttaccaaata gtgtttgtgc taataacact tttacctttt gtccaccagt taattcactc
    37201 atttgctgat ggtgcaattc ttcagggatg ttcagacctt gcagtaaaac agctgcttct
    37261 ggttcagctt cccatccatt aagctcagca aattctcctt ctaattcagc tgctcgaata
    37321 ccatcttcgt cagtgaaatc ctctttcatg taaatcgcat ctttttcttt cagcacttca
    37381 tacagacgct tgtgtcccat gatgactgtt tcaagtacag tatagtcttc atagtcgtaa
    37441 tggttctgtt tcaatacggc catccgttca tctggtccca tagtaacgga acctgtggaa
    37501 ggctgcagat cccctgataa cactttaagg aacgttgatt tacctgcacc atttgcacca
    37561 atcaagccat agcagtttcc gggggtaaat ttaatattca catcatcaaa tagtttgcgg
    37621 tctgaaaagt gcaaactcac atcatttaca gtaatcaatt tttttcctca cttttatcat
    37681 tattagtttt gacaagtctt atagaattat ataggggaaa ggtaaaagat tcaaggcaat
    37741 cgaacattct ttgtgtttca cgtgaaacag aatgaaaaaa ataaaaataa aagcggagcg
    37801 aaaatagcta ctataggatt tattagtttg caacagcata atttttgacg ttatttataa
    37861 gagggagtac tataaaactg taaaagaaat gaaagaagga ttgaaaatgg atgaaaaaag
    37921 aaccatcgac tggggctcat tgattttggg aattttattt gtacttgtct cattgttatc
    37981 atttcaagac cctgtcggaa atttagttgc aattgtagta gtctttgcga tttttgcctt
    38041 tattaaaggg atatttgaac tgtttgtacg gaacagatta aaagagctaa caggatataa
    38101 aggaaagatg cccttaataa tcgggatcat cgatattcta gttggcgtct ttttcttatt
    38161 caatattggt gcaggagtcg cagcattgcc ttttgttttt gcagtatggt ttattgctga
    38221 ttcagtttta gcgttattta ctgctgactt ggcaagagga gtaagtgaag gatattattg
    38281 gtttacgatc atcgtcaata ttttaggaat tttattagga attttcttat tgtttaatcc
    38341 aatttcttct gcgttgacat taagcttttt agttggattc tattttatgt tatttggtat
    38401 cacacatatc gtctatgcat ttagataagt aaaatcaaac aaataaaaaa gccggcttcc
    38461 gattacagtt ttactgttgt aattggaagc cggctttttt atcatggaca aatatgcacg
    38521 aggtgatttt taggtgtcga gacaaattta tgcaaaaatg tgagaagcta tatacacaaa
    38581 ttttatctta atactcgaag catagcgcag ttgtcacaac ctcttttcat ataattcatt
    38641 caatactggc aaatcatgga cttcatattt ttctttgaat gcttggattt cttcttcaga
    38701 atattttctt ggatctaaat gcagatcttc tactggcaat gggcgcttgt tattgatctt
    38761 cacatcgatt actacaggac gatctgattt tgcagctgca tcaaaggcaa atcgaagttg
    38821 cgagtattct gtgactgtaa agctatttgc cccgagcgct tctccaactt taccgaaatc
    38881 tgcatcttct aaaaatacac caaatttctt ttgttccgta tcttcttgtt ctgcttcgat
    38941 gaaaccaaaa gaatcattcg aaaagacaac attaataatt ggaagctgat atttgacttg
    39001 tgtaatgata tcttgcatga ccatagcaaa cccgccatca ccacttaagg taaatacttg
    39061 tttttctggg tagcttagtt gtgcggcaat tcctcctggc acaccattac ccatagttgc
    39121 gaaccatcca gatgtcgtat gtttttgttt accgttcata ttcaataaac gaatagaatg
    39181 gatagtcgtg ttaccgacat ctgtgacgaa aatcgcatcg tcttcagcaa tacgattgat
    39241 ttctttatag acagcctctg gacgaatcgg ttcttcctct cgatcttcaa aagatttcaa
    39301 ccaatttacc catttttctt tattttcttg atttgctagg taccagctat cagcagggcg
    39361 agcatcccct aactcgacaa gtctttgcaa agctgtgttt gcatcaccga ggatcgacaa
    39421 agatgtttca tggcgtcgtc caaattttga cgcatcgata tcgatttgga taaattcagc
    39481 agttggatta aaaaatgcac gtccaaatgg gaagtcgcta ccgacaaata agatcagatc
    39541 ggcttctgcc aatgcttcat ttccgggttt agtagcgaca cgtccagcaa aaccaagaaa
    39601 gttttcataa tcatcaggaa taattccttt tgctagaacg gaagcgacaa caggaactga
    39661 aaaatgttct gagaattctt tgatttgcgg aaatccgttt cgcgttcctt gaccaacata
    39721 aagaactggt ttttttgctt tttcaagata aggcaaagca gctagcaaat ctttttcttc
    39781 tggtaatatc acacttgttt tgtgagtgtg agcggtggag aaaggttgtt cgtcaatttc
    39841 ttcaaaacca agatcaacag ggatagtcac gatagccacg cctttatgct cataggcggc
    39901 tttaatggct tcatctacta catgcggaag actttgtggt gtcatgactg tacgattgta
    39961 tacgctaaca tctgcgaaaa taggattttc atttagttcc tggaaagagt tatagttcat
    40021 ggatgtagaa gcaacttgtc ctaagaacgc tactagaggc acatggtcca tctgtgcatc
    40081 atacaaacca ttgatcaaat gtgtcgcacc aggtcctgca gagccgaaga cagcgccgac
    40141 tttccccgtt aattttgcat ctgcagcggc tgcgagtgcg ccgacttctt catgacgaac
    40201 ttgaatgtag ttgatttcgg ttttttcttg atatagcgca tccataattg aattgaaaga
    40261 tcctccggga atgccgtaaa tatggtcgat accccagctt tcaagaactt ttaccattgc
    40321 gattcctgca ttgatttttg acatggtgtt tcactccttt agcaatgatg atacataaat
    40381 tttttgtatc tagagtatag aactttttat cgaaaatccc aactaattcg tcttttcaca
    40441 aaaatgtagt catgtttttg ggaatatgct agagtagtac atagatagat tcaagagagg
    40501 ggaatggcat ggttgaatgg ctattgagac ttgaaccttg gcagcaagca cttgctggga
    40561 cagcttttac ttattttatg acagctcttg gtgccgggtt ggtttttttc tttaaagaaa
    40621 tcaaaaaaga agtgctaaac ttgatgttag gatttgcttc tggtgtcatg attgctgcaa
    40681 gtttttggtc tttgttagat ccggctatta caaaagcaga agaaaatgga gatattgctt
    40741 ggctagttgt aagtattggg tttggtttag gcggtgtgtt tttatatatg gcagataaaa
    40801 cactgcccca tatgcatttt ggaccacaac atgaagcaga aggattgcct acccatttaa
    40861 aacggacgat cttgcttgtt ttttctatta cattgcataa cattcctgaa ggtttagccg
    40921 tgggggtagc attcggtgca gcagcaactg cggataatcc gacagccgct atattagccg
    40981 ctgtttcggt ggcgctaggt attggtatcc aaaacttccc agaaggagca gctgtatcaa
    41041 tcccgttgcg acaagaaggt ctgagtcgta aaaaggcctt tgtttacgga caagcttctg
    41101 gaatcgttga accaattgct ggagtgattg gagcgctact agtaacgaaa gtggagctgc
    41161 tgcttcctta tgcacttgct tttgccgcag gagcaatgat ttatgtcgtg gtggaagaat
    41221 tgatccctga agcacagcag acattgtcca gcaaacgtca ttttgcagtt tttggcgtca
    41281 tgagtggatt tatcattatg atgattttgg atgtggcatt aggataaata aaaaaaatat
    41341 atatgatata tcaaataggg ccgcgaactt atatcgcgac cctatttttt tagtaagtta
    41401 tatcataatg aagtgaatgg tatcatacat taataatagt tttttctatg tgtatttcct
    41461 ttgatccagc gaacaagcca gatgataagt gcgattggga ttagaacttt gaccgcaaac
    41521 cataaaagcc cgcttaggac actgacaaaa agactgatga cgaagctagc tgcaattagc
    41581 actaaaagaa ccatgaagat atttaagaac atattttttt gacgcataca aaaccctcct
    41641 cttgttcatt ctttactata gaaaaagtat aacagggatt gtttaaaaac acctccaact
    41701 gaagactgat ttttacttca tctacttaaa tgatcaaaca attgcttttt ctctggttta
    41761 tcatctaaaa tagagcggaa aaggaggaga atatcatgcc taaaaatcgt cttgaagcat
    41821 ttacagatgc tgtaatcgcg attatcatga ctattttagt tttagaactt catgaaccta
    41881 acggagacac gtgggaggcg ctcgctgcat taggttaccg attttttatc tatattgtca
    41941 gttttatcat tttagcgatt tattggaaca atcatcatca tttatttcaa gttgtacata
    42001 agataaatgg acgggtctta tgggcgaaca gtttctttgt ttttgcgctt tcgctttttc
    42061 cgtttgcaac ttcatggatc agtgagtaca tcaatagcct ggctccagaa atgacttatg
    42121 gttttgttac cttgaccgct aatattgcgt attatatctt ggcaaaagaa cttgtcaaag
    42181 caaataaagg ccacgagaaa gcattgaagg cgatgttgaa taattacaag aaatcgtatc
    42241 tatcgattgg tttgaatgtg ttaggaattg tcttagggta tttagtagcg ccagtagccg
    42301 tgctgatcgc aaacatcgtg attctagctg cttggatcat cccaagtcgt accatcgaat
    42361 cacaatatcg gtaaaatgat ccgtgtttaa aatttaagag aaaaatatag aaaaaataaa
    42421 taaacgatca aatatccgag tgatactagt attcaaagag ggatgcagat ggatcaaaaa
    42481 agaattcatg taggactgat tactagtttt gtcacgatgc ttatttattt ggatcaaggt
    42541 catttgactt ggacagtagt tggtgtcgta ggatttttga cattattcac agcaataaaa
    42601 aaataaacat ctttatgtaa tctgatgttc tatctattta agaaaagcat gtttacagca
    42661 gtatgattct gttgtaaacg tgtttttatt tgtggaaaat ttaattttta gaaaagtgta
    42721 tcgaaaaata gatagttagt ttttccacag tggataactt cgtaagagat aaaaataaaa
    42781 aattagtcaa agaagggc
//
LOCUS       DAZKLM010000014        42113 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3764
            SAMEA115706913-rid22582563.denovo.014, whole genome shotgun
            sequence.
ACCESSION   DAZKLM010000014 DAZKLM010000000
VERSION     DAZKLM010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706913
            Sequence Read Archive: ERR13196647
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 42113)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42113)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287823.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287823.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,004
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,849
            CDSs (with protein)               :: 2,849
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 41 of 96
            Pseudo Genes (incomplete)         :: 52 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 15 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42113
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706913-rid22582563.denovo.014"
                     /isolate="VRE3764"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            151..798
                     /locus_tag="ACG842_000689"
     CDS             151..798
                     /locus_tag="ACG842_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HFQ0247251.1"
                     /translation="MTPNREDYLKIILELGGDTTKVNNKQIVSSLDVSAASVSEMISK
                     LVKEELVEHSPYQGVQLTASGLQKASSLIRKHRLWEVFLVEHLDYSWNEVHDDAEVLE
                     HVTSEHLADHLENYLNHPEHCPHGGSIPKKEEVVHEERRQTLESYPVGTKVRIARVLD
                     EKELLDYLVTIDLNINEEYEIVDIAAYEGPITIQNDRKKIPVSFKAASTIFVDKI"
     gene            813..2339
                     /gene="zwf"
                     /locus_tag="ACG842_000690"
     CDS             813..2339
                     /gene="zwf"
                     /locus_tag="ACG842_000690"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HFQ0247252.1"
                     /translation="MSNEKNVLFTIFGGTGDLAQRKLYPSLFRLYRKGNLGEHFAVIG
                     TARRPWSDEHYREVVKETIRALNPSDEEATTFASHFYYQSHDVNDSTHYQTLKELSDR
                     LDGHYQLNGNRVYYLAMAPQFFGTIVSHLKSQNIMTKNGFNRLIIEKPFGSDYQSAFE
                     LNQQIREVFPEQDIFRIDHYLGKEMIQNISAIRFANNIFEAQWNNRYIDNIQITFGEN
                     LGVEDRGGYYDHSGALKDMVQNHILQVVALLAMEPPVAFSEKEIRTEKIKALKAIRIY
                     NEEEVLENFVRGQYAQGELDGVQFKGYREEDKVDAQSETETFVAGKFTIDNFRWSGVP
                     FYVRTGKRLTEKGTRINIVFKQVPVNVFKTSVDEPCDDTTLPPNVLTIYIQPTEGFSL
                     SLNGKEVGQGFETEPIKLEFRNSAEMVENSPEAYEKLLLDALNGDGTNFSHWEEVAQS
                     WHIVDVIRKAWDQTQPDFPNYKAGSMGPKAAFELLEKDGFEWIWQPDNWYRERGKLND
                     "
     gene            complement(2388..5186)
                     /gene="ileS"
                     /locus_tag="ACG842_000691"
     CDS             complement(2388..5186)
                     /gene="ileS"
                     /locus_tag="ACG842_000691"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HFQ0247253.1"
                     /translation="MKMKETLQLGKTKFPMRGNLPNREGEWQKEWEENRIYQKRQELN
                     EGKPSFVLHDGPPYANGNIHLGHSLNKISKDIIIRSKSMSGYRAPYVPGWDTHGLPIE
                     QVLANKGVKRKEMSMAEYLEKCREYALSQVDKQRADFKRLGVAGDWEHPYVTLDPSYE
                     AAQVRVFGKMAEKGYIYKGLKPIYWSPSSESSLAEAEIEYKDVKSPSIFVAFNVKDGK
                     DILDEDTAFVIWTTTPWTLPANQGIAVNPKFTYVLVEADGRKFVVAKDLLETVQSEIG
                     WENVTVLKEFAGQEMEYMTASHPFYERESLVILGDHVTLDAGTGLVHTAPGHGEDDYI
                     AGSKYKLSVVSPVDSKGLFTEEAPGFEGIFYDKANPMITDLLKEKGALLKLDFFTHSY
                     PHDWRTKKPVIYRATPQWFASIDKFRQNILDEVEKVEWVIPWGKTRLYNMIRDRGDWV
                     ISRQRAWGVPLPIFYAENGEAIITPETIEHVAKLFAEHGSIIWFEKEAKELLPEGFTH
                     PGSPNGEFTKEKDIMDVWFDSGSSHEAVLRQRPELSFPADMYLEGSDQYRGWFNSSIT
                     TSVAINGVAPYKSVISQGFALDGEGRKMSKSLGNIIAPEKVIKQFGADILRLWVSSVD
                     YEADVRVSMDILSQVAEVYRKIRNTMRFLLANTEDFDPKKDTVSYNDLRSVDKYMTVR
                     LNQVIKEIREEGYDKYNFMHIYRTVMNFLTVDLSSFYLDFAKDVVYIEAEDDYQRRCM
                     QTVFYQTAVALTKLLTPIIPHTAEEIWSFLKEEEEYVQLSEFPGYEEFANQEELMDTW
                     TAFMDFRDNVLKALEEARNSKLIGKSLEAKLTVYPKQQVREMLKALDADIAQLLIVSP
                     DYFEVMAADEQVPDNAMVFDDVAITVEKADGETCDRCRQVRKDVGVDEKLPHLCGRCA
                     KIVEDFYPEAVAEGFEEK"
     gene            complement(5448..6155)
                     /locus_tag="ACG842_000692"
     CDS             complement(5448..6155)
                     /locus_tag="ACG842_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HFQ0247254.1"
                     /translation="MALTPLDIQNKTFPTKMRGYNQDEVDDFLDLVVRDYEELTQRNR
                     ELEKAVKHSEEKLEYFNELKDALNQSIIVAQDTADKVKTSASKESEVIVTSAQNKADE
                     LVANAEKRAHQLTTDAEEKARKILTDATEKARQLATETEDLKKKTRVFHQRISLMLES
                     QLEQVKSPEWDEILQPFSSYVTDSHEVIKEVLAKQLDNENDTDVNSDTGVIEAPMTAF
                     DTQAIDISIVPGENENE"
     gene            complement(6176..6958)
                     /locus_tag="ACG842_000693"
     CDS             complement(6176..6958)
                     /locus_tag="ACG842_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HFQ0247255.1"
                     /translation="MDANVYQHFRADERTFIDSVGDWIEQVQGQYAPYLTEFLDPRQS
                     YILETLIRQSSDLRFMFYGGYEQAERKRCLIFPDYYEPVEEDFETALYEIHYPSKFAS
                     LSHGKILGTLVGTGIKRSYFGDIISDGDRWQVFIANEVDHFVAAQVTKIGKVAVRLEP
                     IKYTEMIMPKDSWTQERGTVSSMRLDSVISEIYNISRQRSKQLIEAGKVKVNWTENTR
                     PDFVLELLDIISVRGYGRVQIQELEGKTKKEKYRVLFGVLRK"
     gene            complement(7007..7228)
                     /locus_tag="ACG842_000694"
     CDS             complement(7007..7228)
                     /locus_tag="ACG842_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HFQ0247256.1"
                     /translation="MVYALLSWFPGGYQSGLGRLLAKICEPYISLFDRLPLRIGPLDF
                     TILGAILILNLAVQGLAQIVIWITVHLVY"
     gene            complement(7309..7908)
                     /locus_tag="ACG842_000695"
     CDS             complement(7309..7908)
                     /locus_tag="ACG842_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HFQ0247257.1"
                     /translation="MPLLNRGAIANFFGLADEEEYEYQDYPETTKKAVQPTKKNTTQN
                     QSTHSVSAANTHEKQKTATSRPVTHESIQPAEKKKVIELHQSPTKTVAKERTASRTST
                     QPKANLAGKITVMEPRAYSEAMAIAKRIIAGEATLVNFHLVEEKQARRIVDFLTGTVY
                     ALDGDIKRVGDEIFLCTPTGMEIDSSTAQSFADANLFDL"
     gene            complement(7922..8599)
                     /locus_tag="ACG842_000696"
     CDS             complement(7922..8599)
                     /locus_tag="ACG842_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HFQ0247258.1"
                     /translation="MIADNLQKIKQEIQDSCALVHRDPKEVTLVAVTKSVESAQAEEL
                     VRAGVMNCAENRVDKLLEKKQDLFDFPEIKWHLIGNLQRRKVKLIVNEIDYFHALDSL
                     RLAEEIQKRLEKELSCFIEVNVSGEESKHGISPDELLPFVESLAACTKIKVVGLMTMA
                     PKEASDEQIHKVFGTLKELRDLVQAKGYAHAPCSELSMGMSQDFQIAIEEGATFIRVG
                     TALFKDE"
     gene            complement(8616..9857)
                     /gene="ftsZ"
                     /locus_tag="ACG842_000697"
     CDS             complement(8616..9857)
                     /gene="ftsZ"
                     /locus_tag="ACG842_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HFQ0247259.1"
                     /translation="MEFSIDNNINDGAVIKVIGVGGGGGNAVNRMIEENVKGVEFITA
                     NTDVQALKNSKAETVIQLGPKYTRGLGAGSQPEVGQKAAEESEQSLREALDGADMIFI
                     TAGMGGGTGTGAAPIVAGIAKELGALTVGVVTRPFTFEGPKRGRFAAEGIARLKENVD
                     TLLIISNNRLLEVVDKKTPMLEAFREADNVLRQGVQGISDLITAPGYVNLDFADVKTV
                     MENQGTALMGIGVASGEDRVVEATKKAISSPLLETSIDGAEQVLLNITGGLDMTLFEA
                     QDASDIVANAATGDVNIILGTSINEEMGDEIRVTVIATGIDESKKERKSSRPARQAQM
                     QSPAQKTVLDMDQAKPTSSEEENSFGDWDIRREQNVRPRVDDSNFDNIEKKEFDTFNR
                     EETKSHGDDELNTPPFFRRKK"
     gene            complement(9880..11205)
                     /gene="ftsA"
                     /locus_tag="ACG842_000698"
     CDS             complement(9880..11205)
                     /gene="ftsA"
                     /locus_tag="ACG842_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HFQ0247260.1"
                     /translation="MAKTGMYVGLDIGTTSVKVVVAEYIDSQMNIIGVGNAKSEGINR
                     GIIVDIDKTVQAIQRAVRQAEEKAGIQIKGVSVGLPANMLEVENCQGMIAVNGDSKEI
                     TDEDVRNVASAALVRSIPPERQIVSILPQDFTVDGFEGIKDPRGMIGVRLEMYGLLFT
                     GPKTIIHNIRKCVENAGLIVNEMVITPLALTESILSDGEKDFGTIVIDMGGGQTTTAV
                     MHDKQLKFTNLDQEGGEFVTKDISIVLNTSFNNAEALKINYGDAYPERTSPDEEFPVD
                     VIGQSEPVKVDERYLSEIISARMEQIFNKAKDALDQIEALELPGGVVLTGGAASLPGV
                     VDLAQEIFGVNVKLYVPNHMGLRNPVFTNVISIVDYSANLSEVYQLAKIAVTGETSAA
                     RQMVVEQETTNTYESYEAPEETIYDEPEPKESGENVKNKIKGFFTNIFD"
     gene            complement(11361..12581)
                     /locus_tag="ACG842_000699"
     CDS             complement(11361..12581)
                     /locus_tag="ACG842_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HFQ0247261.1"
                     /translation="MSKKKEPKKLSHPSSDENNLTPWQKANRKYLAEQEMKNQEPKKE
                     DITLDTPLSDSEEEKTVQEEQQEESQSEKKIEFFEEIKQKKNGGPYNGSFLNRLPNLK
                     SQRHKVLFRRLALIITVLGIPLIFLIYYVSPYSKLQAVAVSGNKTVNSQEIISDTKLS
                     LGENVWGQYFHRSTYIDRLKKAQPRIETANIHFKGMNEFDLDVTEYKEIALIAKNNQY
                     YPVIENGTVLDEKVANPTKNLPILEEFKDSAKIKELTKQYNQLSSELQKAISEIKYTP
                     RASNKNLIQLNMNDGNQVIVNINNLANQMKYYSQVAKDMDEKGVIDMEVGIFSHPYGS
                     TQETEESGQSEETTESEENADQTNESSEEAETQKAQTPGSSSTNGSSDSNDSSETSQI
                     EDNPSTTPSSLNEN"
     gene            complement(12596..13684)
                     /gene="murG"
                     /locus_tag="ACG842_000700"
     CDS             complement(12596..13684)
                     /gene="murG"
                     /locus_tag="ACG842_000700"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HFQ0247262.1"
                     /translation="MKILVTGGGTGGHIYPALAFVNYVKTQEPNAEFMYVGAKRGLEN
                     KIVPDTGMPFHTLEIQGFKRKISMHNIKTVRLFLKSIRQAKKILSDFQPDIVIGTGGY
                     VSGAVVYAAAKMGIPTVIHEQNSVPGVTNKFLSRYVDRIALSFKDAASFFPENKAVLV
                     GNPRAQEVADTKKSDILKTFDLDPEKKTVLIFGGSQGALKINQAVSVFLSEYIPSSYQ
                     VLYASGDRYYEEILNKINPEIQNISVQPYIKNMAEVMASSDLLVGRAGATSIAEFTAL
                     GLPAVLVPSPYVTNDHQTKNAMSLVHAGAAKMIADNELTGESLSQTVNEIMGDEELQK
                     QMCRASKEQGIPDASKRLYDLVKQIIKK"
     gene            complement(13710..15071)
                     /gene="murD"
                     /locus_tag="ACG842_000701"
     CDS             complement(13710..15071)
                     /gene="murD"
                     /locus_tag="ACG842_000701"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HFQ0247263.1"
                     /translation="MKKISTYENKKVLVLGLAKSGVSAAKLLHELGALVTVNDGKPFD
                     ENPEAQELLSLGIKVITGSHPIELLDEEFSLMVKNPGIPYSHPLVAKAQEMGIPVITE
                     VELAYEVAECPIIGITGTNGKTTTTTMTGLLLNAGADQGIARLAGNIGYPASGVAQEA
                     KSEDKIVMELSSFQLMGITDFRPHIAVITNIYEAHIDYHGTRKEYVKAKWNLQKNMTE
                     KDYLILNWNQSELQELAQRTKARVLPFSTKEVLEDGVYADDYSIYYKKEKIMEISELG
                     VPGKHNVENALAAISVAKLYGISNEAIRETLHFFHGVPHRTQYVGEIQGRKFYNDSKA
                     TNILATKMALSGFETSKVVLLAGGLDRGNTFDELIPSLKGIKAMVVFGQTKEKLMDAG
                     KKAGIETIVTADSVEQAVPLALENSTDGDVVLLSPANASWDQYPNFETRGNRFMEAVN
                     RLK"
     gene            complement(15085..16047)
                     /gene="mraY"
                     /locus_tag="ACG842_000702"
     CDS             complement(15085..16047)
                     /gene="mraY"
                     /locus_tag="ACG842_000702"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HFQ0247264.1"
                     /translation="MEWTQALIPIVSSCAMTIAAMPLFIGYFQMKKQGQAIREEGPKW
                     HNSKAGTPTMGGLVFLIGSILTGIWVGAWQKQLTPTLFILLFVLALYGVIGFLDDFIK
                     IFKKRNMGLNSKQKLIGQILGGIIFYLVYRSEGYPGVLNFFGLDLPLGIIYGIFALFW
                     LVGFSNAVNLTDGIDGLVAGLGSISFAAYGLIAWHQHQYDVLIICLSVLGGLLGFFVY
                     NRKPAKIFMGDVGSLALGGLLAAISIMLNQEWTLLLIGLIYVMETASVMLQVTSFKLT
                     GKRIFKMSPIHHHFEMCGWSEWKIDTVFWLISIVTSLITLWIVW"
     gene            complement(16073..18265)
                     /locus_tag="ACG842_000703"
     CDS             complement(16073..18265)
                     /locus_tag="ACG842_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HFQ0247265.1"
                     /translation="MSLKNKIRRFMEKKNLNPMNNRKKVGIILFATSIGLFFLFAVRF
                     SYIVIGGHVAGTSLAEKTKQLYQGSEVVKAKRGTIYDRNGVALAEDASSYSIKAILSK
                     TYTSGDKKLYVEEKNFDKIAEILHKNLSIDKKDALNILEDGAKKELYQVEFGSYGKNI
                     SQETKQNIEADMKKEGVAGLYFVDHQARMYPNGVFSSHFIGYAVPDKDENGLVGKLGL
                     ESAYNDILSGKDGKIIYQKDNFQNPLPGTVAEEEKAVDGQDIYTTLDSRLQSYLETLM
                     DQVNEEYQPEELTAVLMKAKTGEILAMGQRPTFNPETMEGLTGKDAIWRNFLVQDSYE
                     PGSTMKVFTTAAAIEEGEFNENETFQSGKIQVEDATINDHDFGEKGVLTMRQALSWSS
                     NVGMVILEQRLGGRWYNYLQKLGFGQSTHSGLDDEVNGALPTLNIVDRAMSAYGQAVG
                     VTNFQMMKAFTSIANNGTMIQPRYISKVVDPQTGEERTTQTEVLGQPFSKETTEKVRE
                     YMRDVVESENYGSAYGVYSVPGYNVSAKNGTAQIASDTGGYQTGDTAYLYSIVEMVPS
                     EDPDYVLYLTMKHPKTYDRMALAKIANPLMKRAMDFKETEEDSDTETKTEKVSVADYR
                     NLEADVAAADAQKSGLQPVVIGNGKKVQKQSTANGDQLISGEKLILYTGGDKLMPDVT
                     GWSKADIMKLGKILGIEVSFDGDGYCVKQELAPYEKITEDKLSFTLEE"
     gene            complement(18266..18667)
                     /gene="ftsL"
                     /locus_tag="ACG842_000704"
     CDS             complement(18266..18667)
                     /gene="ftsL"
                     /locus_tag="ACG842_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HFQ0247266.1"
                     /translation="MAELKKMQDYEYDLPELVDQPEQQMPDSSSNQEIILPQSPAKRL
                     KQISALEKIAVASVAIAIVALCVLTVMLRTNISGAEKEITSIQTEINDKKQEKTSLEQ
                     EKNELSRTERIKQIAEEKGLSINDDNLRKVK"
     gene            complement(18672..19631)
                     /gene="rsmH"
                     /locus_tag="ACG842_000705"
     CDS             complement(18672..19631)
                     /gene="rsmH"
                     /locus_tag="ACG842_000705"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HFQ0247267.1"
                     /translation="MTETFQHYTVMLKETVDGLNIKPDGVYVDCTLGGAGHSEYLLSQ
                     LSSKGHLYAFDQDQKAIDHAKLRLASYVEQGMVTFIKANFRELEERLSEHVDKVDGIL
                     YDLGVSSPQLDEAERGFSYHQDAPLDMRMDQSAPLSAYHVINEYSYHELVKIFFRYGE
                     EKFSKQIAREIERVRKTKPIQTTGELVEIIKQVIPAPARRKGGHPAKRIFQAVRIAVN
                     DELGAEEASLEQAIRLLKVNGRISVITFHSLEDRIVKSMFKEYSTVQDLPPGLPVIPD
                     EFQPELKVITRKPILPSEEELTENNRSRSAKLRIAEKIRMKEE"
     gene            complement(19652..20083)
                     /gene="mraZ"
                     /locus_tag="ACG842_000706"
     CDS             complement(19652..20083)
                     /gene="mraZ"
                     /locus_tag="ACG842_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005881057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HFQ0247268.1"
                     /translation="MLMGEFQHNIDAKGRLIVPSKLREELGEKFVLTRGLDGCLFGYP
                     MSEWENLEAKLNEMPLAKKDARTFVRFFYSAATECELDKQGRINIPSTLRNYAALTKG
                     CVVIGVSNRIEIWDETRWQDFSAAAEENFDEIAESMIDFGL"
     gene            complement(20253..20621)
                     /locus_tag="ACG842_000707"
     CDS             complement(20253..20621)
                     /locus_tag="ACG842_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3397 domain-containing protein"
                     /protein_id="HFQ0247269.1"
                     /translation="MGTFTPLILFWYIYPVIVLFGCQFLVKLFSLNRRFKIKAPDLAI
                     PFLWGGMHALSRNTGTISILPFFFISVLLMGICIAVFQAYYYEEIIYPRYFKMTWRLV
                     FLLTIVLYAVLIIVNILSYL"
     gene            20792..21748
                     /locus_tag="ACG842_000708"
     CDS             20792..21748
                     /locus_tag="ACG842_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HFQ0247270.1"
                     /translation="MIKYFTLDNEKLRNTGKETDQTIWYCVERPNQEEIHQLTHQFEI
                     PSDYITSILDDAENSRTEEFNQSKLTKPALLLLQYPFAKTSPSGYFQVDTYPLSIILT
                     PKKKLITITNHEPLFLKKVFSQTFPKSDISPNLNIFFQFIWQVTLSYNSYLATLQSQC
                     DTLEAKLQVSTENKQLYQIMDIQKSLVYFREATSANLDALKELAQNKLFQENHALKNH
                     LRDVLVETEQAMTTSRIQLKLVEQMNQTFSAIVSNNLNNIMKILTSLTIILTIPTIVG
                     SIYGMNIKLPIAERDDAFIWLVLLTLFICIVTTYYLKKGKFL"
     gene            21821..21937
                     /locus_tag="ACG842_000709"
     CDS             21821..21937
                     /locus_tag="ACG842_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4044 domain-containing protein"
                     /protein_id="HFQ0247271.1"
                     /translation="MREKKQTSTFAKVTKFVIWTMLILTIGSVVLTAVMSVM"
     gene            complement(21992..23674)
                     /gene="recN"
                     /locus_tag="ACG842_000710"
     CDS             complement(21992..23674)
                     /gene="recN"
                     /locus_tag="ACG842_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HFQ0247272.1"
                     /translation="MLQEISITNFAIIPELRLSFHEGMTALTGETGAGKSIIIDALGL
                     LAGGRGSSDYIRQGAEKCVLEGLFELPKQEGFSELMVELGIETDEDNLIVRRDMSLTG
                     KNVCRVNGHIITLANLRRIGSYLVDIQGQNEHQELLQPESHLALLDRFGDVAFQQKKK
                     SYQQEYISYRELEKRVRKVQQNEKSYVQRIDMLHFQQEEIAAADLQVGEEEKLKEERE
                     KLSNYQKIADGLAAGYGALTNNEQNSVDGVGLAVSELQGIAHLDVEYEAIYENIQSAY
                     YLLQDAIGDMSRQIDLLELDESRLEEVTQRLELIRQLKRKYGESIESILAYYDEITEE
                     LASSDFSEGQLDKMKEELEQKELLLQQQAADLHEARKKIAKELEKSILHELKSLYMEN
                     TEFEVRFLKEENRQLNHDGFDQIEFYITTNPGEPLKPLVKVASGGELSRMLLALKTIF
                     SSEQGVTSIIFDEVDTGVSGRVAQAIADKISQISKYSQVLCITHLPQVAAVADYQYYI
                     VKAVIDGRTQTSVSELKTKEREEEIARMLAGSEITKLTLEHAKELLKLAKRT"
     gene            complement(23692..24141)
                     /locus_tag="ACG842_000711"
     CDS             complement(23692..24141)
                     /locus_tag="ACG842_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HFQ0247273.1"
                     /translation="MRKKDRHRLITRLLSEQDIRKQEEFVEILKSKGISVTQATISRD
                     IKELKLIKVPASDGGYRYSIPAETNEDVNTKLEKLLKDAFVAVDQMEKFVILKTLPGN
                     ASAAANLIDKRYKKELFSIINDDDNVLMITRTEDQAVLLKKDFLHYL"
     gene            complement(24248..25066)
                     /locus_tag="ACG842_000712"
     CDS             complement(24248..25066)
                     /locus_tag="ACG842_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HFQ0247274.1"
                     /translation="MEKERVDVLAVKQGLFETREQAKRSVMAGLIYNEKNERFDKPGE
                     KIPVSSELRVKGKKLPYVSRGGLKLEKALKAFDLSVEGKTLLDIGASTGGFTDAALQN
                     GAKMSYALDVGYNQLAWKIRQDPRVVVMERVNFRYAKPEDFTLGIPEVAVIDVSFISL
                     KLMLPPLHAILKEEGEVIALIKPQFEAGREAVGKNGIVRDPQTHQKVLEDILSFAAQG
                     GYDVLELSYSPITGGEGNIEFLAHLRKVPESGTINSAINMAEVVSNAHEQFDHK"
     gene            complement(25075..25965)
                     /locus_tag="ACG842_000713"
     CDS             complement(25075..25965)
                     /locus_tag="ACG842_000713"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HFQ0247275.1"
                     /translation="MRLTDFSAIHLPAVEKEILSFLDEHTTDRGLYDAMSYSVKAGGK
                     RIRPLLLLTAVASFDEPIDVPVYQVAAALEMVHTYSLIHDDLPAMDNDDLRRGKPTNH
                     KVFGEALAILAGDGLLTGAFQLISMAHLGNSPKLLLLQQLAVCAGSQGMVAGQAADIE
                     GESKKLSLEELAFIHERKTGHLIRYALLAGGILAKQPEEILLLLQRLAEHLGLAFQIR
                     DDLLDVIGTTKTLGKTAGKDERMEKNTYPRLLGLEKTREALEIELLSANKIIDKLEED
                     VFSFDGGLMRQMIKQFDVEK"
     gene            complement(25967..26191)
                     /locus_tag="ACG842_000714"
     CDS             complement(25967..26191)
                     /locus_tag="ACG842_000714"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HFQ0247276.1"
                     /translation="MENPTFEESLQELEKIVMQLEQGDVPLESALDSFKRGMELSKHC
                     QDTLAKAEETLTKMMTESNEEVDFDGNEES"
     gene            complement(26196..27533)
                     /gene="xseA"
                     /locus_tag="ACG842_000715"
     CDS             complement(26196..27533)
                     /gene="xseA"
                     /locus_tag="ACG842_000715"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HFQ0247277.1"
                     /translation="MAQEYLTVTTLTKYLKRKFDADPYLERVYLTGEISNFRLRANAH
                     QYFSLKDDHAKISAIMFKGAFQKLRFQPKEGMKVLVVGRISLYEAGGSYQIYVEHMEP
                     DGIGALYQELEERKEKLMKEGLFQGPKKMLPRFPKRIAVLTSPSGAVIRDIITTVKRR
                     YPIVQLVLFPTVVQGNQAADNVVRNIQRVEEMGNFDTMIIGRGGGSIEDLWPFNEERV
                     ARAIYQANTPVISSVGHETDTTIADLVADVRAATPTAAAELAVPVLSEELMRIQERQA
                     RLQQAFVRQIQRKQERFERARRSYVFRQPERLYEGQTIKLDQLKQRLYQATQQIYHAK
                     EKQTIALSHQLEQVAPLYRVKAARQETAYLEKRLEEKIQQYMKQQQQRFAQAVQSLDL
                     LSPLKIMGRGYSYTTLNDQVVKSVTEIHQGDLLTIHYQDGIVKGQVQEIDKGE"
     gene            complement(27534..28388)
                     /locus_tag="ACG842_000716"
     CDS             complement(27534..28388)
                     /locus_tag="ACG842_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HFQ0247278.1"
                     /translation="MAELINGKELAEKMQAEIAEKIQKLKDNGIHPGLVVLLVGENPA
                     SQIYVRNKERVAKEIGIHSLVERYPETISEDELLAEIEKYNQDPSFHGILVQLPLPKH
                     IDEEKILLAIRPEKDVDGFHPMNLGRLLAGKPDKIPCTPYGIMKMFEAYSIDPAGKRA
                     VVIGRSNIVGKPMAQLLLMADATVTVAHSKTENLAELAQEADILVVAIGRGHFVTKEF
                     VKPGAVVIDVGMNRDANGKLIGDVKFDEVEPIAGWITPVPKGVGPMTITMLMYQTVKS
                     AEAFGAGE"
     gene            complement(28485..28934)
                     /gene="nusB"
                     /locus_tag="ACG842_000717"
     CDS             complement(28485..28934)
                     /gene="nusB"
                     /locus_tag="ACG842_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HFQ0247279.1"
                     /translation="MNKELSRHEIREMALQALFPLDFNADLTKEDAIFNAIELDHRDM
                     INEDESEFVPVYLDTLVGGVCAKKDELDKVIEKHLKNNWHINRISKMDLVILRIAIFE
                     MTYVTDVPAAVALNEAIELAKTFSDDRSRKFVNGVLSNVLKELEAGA"
     gene            complement(28927..29352)
                     /locus_tag="ACG842_000718"
     CDS             complement(28927..29352)
                     /locus_tag="ACG842_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HFQ0247280.1"
                     /translation="MADEKNLILNANQDLGEIVIAPEVIEVIIGIAASKVEGVYGMRG
                     TFASNVTELLGRAAHGKGVYLANEEDGLKVDLYCYLEYGVSVPKVAMEMQERVSQQVL
                     FMTDIDLSEVNIHVVAVVPEKLEQPTIEELFEDEEEENE"
     gene            complement(29598..30662)
                     /locus_tag="ACG842_000719"
     CDS             complement(29598..30662)
                     /locus_tag="ACG842_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HFQ0247281.1"
                     /translation="MMLRVEKLRKKMQEENLDSFLVTSPYNLRYLTNFTGTTGLAVIT
                     LEKAFFITDFRYTEQAAAQAQGFEIIKNVGPIFDEVANLVRKEELHSLAFEETTVSFL
                     EYSVLEEIIDAELVPVSQMIEELREVKDEEEIAIIEKACHIADMAYDHILKMIQPGMT
                     EIEVANQLDFYMRSLGASGVSFDTIVASGLRSAMPHGVASKKIIEQGDLITLDFGCYY
                     EGYVSDMTRTFAIGDPGEKLKEIYQIVLDAQLAVIDAAKPGMTGVQLDAVARDYISKH
                     GYGEAFGHSTGHGIGLEIHEGPNVSSRAEKQFVPGNVITDEPGIYLPGIGGVRIEDDL
                     LITEDGNRVLTRSPKELIIL"
     gene            complement(30834..31079)
                     /locus_tag="ACG842_000720"
     CDS             complement(30834..31079)
                     /locus_tag="ACG842_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFQ0247282.1"
                     /translation="MSWLWTLIVGAVIGAIAGAITSKGNSMGCVTNIIAGLVGSSIGQ
                     AIFGEWGPKAAGMALIPSILGAVILVAVVSFFFGKRS"
     gene            complement(31218..31592)
                     /locus_tag="ACG842_000721"
     CDS             complement(31218..31592)
                     /locus_tag="ACG842_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF956 family protein"
                     /protein_id="HFQ0247283.1"
                     /translation="MVQSINTKVDLVIDATSFTGLTDYGQIMIGNNGFEFYHARDRRK
                     YIQIPWEEVDYVLASVMLKGKWIPRYAIQTKKNGTYTFSSKEPKKVLRAIREYVDEDR
                     MVQSLSFFDVVKRGIRSMFKRK"
     gene            complement(31675..32589)
                     /locus_tag="ACG842_000722"
     CDS             complement(31675..32589)
                     /locus_tag="ACG842_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFQ0247284.1"
                     /translation="MAEEKIKLSKKDRLAVAWRSTFIQGSWNYERMQNGGWAFSMIPA
                     IKKLYKTKEERSAALKRHLEFFNTHPYIASPILGVTLALEEERANGAPVDDVAIQGVK
                     VGMMGPLAGVGDPVFWFTVRPMLGALGASLAMGGNILGPIIFFLGWNIIRWAFMWYTQ
                     EFGYKAGSKITDDLSGGLLQDVTKGASILGMFVLAALVQRWVSIKFLPVVSTVKLDKG
                     AYIEWDKLPSGGEGIKSAFEQVNNGLALSPEKVTTLQDNLDQLIPGLAALGLTLLCMW
                     LLKKKVSPIVIILGLFVVGVVGHVIGLL"
     gene            complement(32610..33413)
                     /locus_tag="ACG842_000723"
     CDS             complement(32610..33413)
                     /locus_tag="ACG842_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HFQ0247285.1"
                     /translation="MSIISIILVVLVAFLAGMEGILDEFQFHQPLVACTLIGLVTGNL
                     EAGIVLGGTLQMIALGWANIGAAVAPDAALASVASAIILVLGGQGVKGVPSAIAIAVP
                     LAVAGLFLTMIVRTIAVPIVHLMDAAAEEGNIRKVEMWHIIAVCLQGVRIAVPAAALL
                     FIPAQTVQSFLESMPAWLTDGMAIGGGMVVAVGYALVINMMATKEVWPFFVIGFVVAA
                     ISQLTLIALGALGVALALIYLNLSKMGGSSNNGGGSNSGDPLGDILNDY"
     gene            complement(33446..34435)
                     /locus_tag="ACG842_000724"
     CDS             complement(33446..34435)
                     /locus_tag="ACG842_000724"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose/fructose/sorbose PTS transporter subunit
                     IIB"
                     /protein_id="HFQ0247286.1"
                     /translation="MVGIILASHGQFAEGILQSGSMIFGEQENVKAVILKPSEGPDDL
                     RAKLEEAVASFDNQDEVLFLVDLWGGTPFNQSNTLFEEHKDKWAIVSGLNLPMLIEAY
                     ASRFSMESAHEIAAHIIETAKDGVKVKPEELEPAEAPKAAVEDAQPKGALPEGTVVGD
                     GKIKYVLARVDSRLLHGQVATAWTKAVQPNRIIVVSDAVSKDDLRKRLIEQAAPPGVK
                     ANVIPISKMIEVAKDPRFGNTKALLLFENPEDVLTAVEGGVDIKELNVGSMAHSVGKV
                     VVSKVLSMGQEDVEAFEKLEQKGVKFDVRKVPNDSRDNMDDILKKAKAELAKA"
     gene            complement(34538..35029)
                     /locus_tag="ACG842_000725"
     CDS             complement(34538..35029)
                     /locus_tag="ACG842_000725"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose/fructose/sorbose PTS transporter subunit
                     IIB"
                     /protein_id="HFQ0247287.1"
                     /translation="MDIRLARIDDRLIHGQVATVWSKQTGVERIIVISDVVAQDKLRK
                     FLLKEAAPPGITANVITVQKMIQIYHSGLLSRAKVMLLFTNPQDVEAVVRCGVQLKSL
                     NIGGMSFTEGKSMITNFVSVNQQDIDSFRYLNSQEVELEIRKVPADRKILLMELLAKI
                     KKV"
     gene            complement(35220..38027)
                     /locus_tag="ACG842_000726"
     CDS             complement(35220..38027)
                     /locus_tag="ACG842_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFQ0247288.1"
                     /translation="MYVKEKTAHLTKAEYDQGVTTQEIAEVLGIQRTNSSKDLNQLVR
                     EGKLIKTDGRPVRYIYQTLVTHVKPVTKHVVSYKETPVSEEKNTLQIDTKDIFAKIIG
                     ANGSMKNSVEQAKAAILYPPRGLNCLITGPTGSGKTYFAHAMYHFAKAHHVIDEETEL
                     IVFNCADYANNPELLMSHLFGYAKGAFTGADEEKMGIIDQADGGMLFLDEIHRLPPEG
                     QEMIFYFMDHGTYSRLGETTKSHEANVRIVGATTEDPGSSLLETFVRRIPINIKLPAF
                     EKRPANEKVDLVKIMIAHEANRTQRKISLTEDVAKALIGSVTYGNIGQLKSNIQLVCA
                     RGFLNHMQSPEISITIDDLTEGIRSGLIQLASNRSAMAELSKILEPKITVAPNDTIMK
                     IQSDSYELPYNLYDIIGDKAALLKSDGLDQEAINHFISTDINIHLKSFYKDHGFSFNA
                     DSKLAEFVDPKIIEVTNQIYVMVKRSLPYEFQQNFIYAMSLHISSFLKKINLGEERHT
                     NDNIREMAFDFPDEYAVAKEVRSYIETYFQVEIPESEDYYLTVLLVSLRANQASGRIG
                     VVVAAHGNSTASSMVQVVQQLLDAENVRAVDMPLDMDPKTALDRIERNVQEVDEGSGV
                     ILLVDMGSLASFNSQIQRDTGIPVRTVDMVTTSLVLETVRKVSVLGTDLDMLHESLKN
                     FRGYAEISSVESVQEAKPIHKKKAVLAICASGEGTAQRIKELIERAVNKRQETELEVL
                     ALSIVEVKEELPVIQKEYQIIATTGITDLNIQAPFIPLERFIDQNIEVILDQLLMESE
                     LEETEFISLDEESAKNTCVEFISDNFIFINGSKLIDPMWQFSTQISQTTGIGDEEYGF
                     KINLVMHTAGMIERIIRNEPLTVEENELTNTTNDPLYSQLAASVVLLEDQIKVKVPIE
                     EMYYLLRLVHNQLDKKEYTVP"
     gene            complement(38461..38754)
                     /gene="rpmA"
                     /locus_tag="ACG842_000727"
     CDS             complement(38461..38754)
                     /gene="rpmA"
                     /locus_tag="ACG842_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HFQ0247289.1"
                     /translation="MLLSMNLQLFAHKKGGGSTSNGRDSESKRLGAKRADGQTVTGGS
                     ILYRQRGTKIYPGVNVGIGGDDTLFAKVDGVVRFERKGRNKKQVSVYPVAQEA"
     gene            complement(38767..39111)
                     /locus_tag="ACG842_000728"
     CDS             complement(38767..39111)
                     /locus_tag="ACG842_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HFQ0247290.1"
                     /translation="MIKGTFKRNDAGQIVSFTLTGHADAGPYGSDIVCAGVSALAIST
                     VNGIASLAGFEPIVEMNEEEGGYLYTEVTSGMTQEQNNIAEILLENLLLGLQSIEAEN
                     SEYIQIKTINDK"
     gene            complement(39138..39446)
                     /gene="rplU"
                     /locus_tag="ACG842_000729"
     CDS             complement(39138..39446)
                     /gene="rplU"
                     /locus_tag="ACG842_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HFQ0247291.1"
                     /translation="MYAIIKTGGKQVKVEVGQAIYVEKLDVEAGEKVVFDEVILVGGE
                     STKVGAPTVAGATVEGTVEKHGKQKKVVTFKYKPKKHSHRKQGHRQPYTKVVIDAINA
                     "
     misc_feature    complement(39466..39542)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00559"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ribosomal protein L21 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00559"
     gene            39728..40369
                     /locus_tag="ACG842_000730"
     CDS             39728..40369
                     /locus_tag="ACG842_000730"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HFQ0247292.1"
                     /translation="MEYPKELIQEASLRMNGRPVEGFIAGQGPLHPKLMLVGEAPGKT
                     EIDTHVPFSGQAGKELMQALSSTGLTREEVYITSAVRSRPYRVTHRINKRTQQSETVY
                     PNRTPTRSEVFAHAPILDYELMKVQPKLIATLGNIGLQRLLGKEYTVTKNHGQLYTGP
                     VLQLTEQKDAYEWSEKNYRILPLFHPAAIFYNRSLVPVIHQDWQVLASLLQQY"
     gene            complement(40431..42062)
                     /locus_tag="ACG842_000731"
     CDS             complement(40431..42062)
                     /locus_tag="ACG842_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015103 - inorganic anion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HFQ0247293.1"
                     /translation="MFKNYVSSLKKEFSGYDLSRFQKDLLAGITVAAVALPLALAFGV
                     SSGADAAAGLITAIIAGIVIGGLSGGFYQISGPTGAMAAILMSIAATKGMQGVLLATF
                     LAGLFLLAAGLFRLGTLTSFIPAPVITGFTSGIAIIIALGQIDNLFGVHSEGTNVIEK
                     LSSYQTIGFPINTASLIMGGLVILGMFIYPKKWAKRMPSSLLAIILATAVMLLFHLPV
                     ATVGKIPQTLISSNRLNMGDFSFSALQQVAVPAISIALLGMIESLLCGASAGRMANKP
                     LDSNQELMAQGIGNLLLPFFGGIPATAAIARTSVAIKSGAQTRLAGMIHAVVLLLSML
                     VFAPVMSQIPMPALAGVLIVTAWRMNEWETIKELFTNKYWSAVLLFILTMICTVIFDL
                     SIAIVIGVIGGCVFFVVKSAAITISLETIDWQRMGLPETEKLNNWTVVYISGPLFFMS
                     SEKLKNKLEELNHHDGVIFSMRGVPSIDLTARAVFDEFQEKAEQRGQTIIYTSLQPEV
                     EKQLSHLWEHTFTHQHQTVAHALTALHAKYTAISH"
BASE COUNT        11868 a         9170 c         7157 g        13918 t
ORIGIN      
        1 acgaaagaat gagtcttctc tatcatcttt cgttttttgt tgtaaaaacg tctaaaaatc
       61 ctttgttttt tatttagaaa aatagtcaat taacgttttt tttgttacac ttactaatga
      121 tggtttttta ccatgaaagg atgatcgaat atgacaccaa atcgagaaga ttatttgaaa
      181 atcattttag aactaggtgg agatacaacg aaagtcaaca ataaacaaat cgtatctagt
      241 ctagatgtct cagctgcatc tgtcagtgaa atgatttcta aacttgtgaa agaagagctt
      301 gtagaacatt ccccttatca aggtgtacaa ttgactgcaa gcggactgca aaaagctagc
      361 agtctgattc gcaaacatcg cttatgggaa gtttttttag ttgaacattt agactatagc
      421 tggaatgaag tccatgatga tgctgaagta ttggaacatg tgacttcgga acatcttgca
      481 gatcatttag aaaactattt gaatcatcct gaacattgtc ctcatggagg atcgattcct
      541 aaaaaagagg aagttgtcca tgaagaacgc cgacaaacat tggagtccta tcctgtcggt
      601 acaaaagtgc gtatcgcacg agtgcttgat gaaaaagaat tgttggatta cttagtcact
      661 attgacctga atatcaacga agaatatgag atcgttgaca tcgcagctta tgaaggaccg
      721 atcactatcc aaaatgatag aaagaaaatc cccgtcagct ttaaggcggc cagcacgatt
      781 tttgtcgata aaatataaag gagtgtatcc acatgtctaa tgaaaaaaat gtattgttta
      841 cgatattcgg cggtacaggt gacctagccc agcgtaaatt gtacccgtct ttgttccgcc
      901 tttacagaaa aggaaattta ggcgagcatt ttgctgtaat cggaaccgct cgccgtccct
      961 ggagcgatga gcactaccgt gaagttgtca aagaaacaat ccgtgcattg aatccttcag
     1021 atgaagaagc aactaccttt gcaagccatt tttactatca atcccatgat gtaaatgatt
     1081 caacgcatta tcagacatta aaagaattgt ctgaccgatt agatggacac tatcaattaa
     1141 acggtaaccg tgtctattat ttggctatgg cacctcaatt ttttggaacg atcgtttctc
     1201 atttgaaatc acaaaatatt atgacaaaaa atggcttcaa tcgtttgatc atagaaaaac
     1261 cgttcggttc tgattaccag tctgcttttg aattgaacca gcaaatcaga gaagtattcc
     1321 cagaacaaga tattttcagg attgatcatt atttaggaaa agaaatgatc caaaacatct
     1381 cagctatccg ttttgccaat aatatttttg aggctcaatg gaacaatcgc tatatcgaca
     1441 atatccaaat tacttttggc gaaaacctcg gtgtagaaga tcgcgggggc tactacgatc
     1501 atagtggcgc acttaaagac atggtgcaaa atcatatttt acaggttgta gcattacttg
     1561 caatggaacc gccagtagca ttttccgaaa aagaaatccg tacagaaaag atcaaagcat
     1621 tgaaagctat ccgcatttat aacgaagaag aagtgttaga aaacttcgtt cgcgggcaat
     1681 atgctcaagg tgaattggat ggtgtacaat tcaagggcta tcgcgaagaa gacaaagtgg
     1741 atgctcaatc agagactgaa acattcgtcg caggcaaatt cactatcgat aacttccgtt
     1801 ggtcaggtgt tcctttctat gtacgtacag gcaaacgttt gactgaaaaa gggactcgga
     1861 tcaacatcgt attcaaacaa gttcctgtta atgtattcaa aacatctgtc gatgaaccat
     1921 gtgacgatac gacacttcca cctaatgttc taacgatcta tattcagcca actgaaggat
     1981 tttctttatc cttgaatggg aaagaagtag ggcaaggatt tgaaacggaa ccaatcaaat
     2041 tggaatttag aaatagtgca gaaatggttg aaaatagccc agaagcttac gaaaaattac
     2101 tattagatgc gttgaatggt gatggaacga acttctctca ttgggaagaa gtcgctcaat
     2161 cttggcatat cgtggatgtc attcgaaaag cttgggacca aacacaaccg gattttccca
     2221 actacaaagc tggatcaatg ggacctaaag cagctttcga actacttgaa aaagacggat
     2281 tcgaatggat ctggcaacca gataactggt atcgtgaacg tggaaaatta aacgattgat
     2341 aacaaaaggg ggtgtgacaa agaacatgtc acaccccctt ttccctttta cttttcttcg
     2401 aatccttcag ctacagcttc aggatagaag tcttcaacga tttttgcaca acgaccgcat
     2461 agatgaggaa gtttttcatc tactcctaca tctttacgaa cttggcgaca gcgatcgcaa
     2521 gtttccccgt ctgctttttc cactgtaata gcaacatcat caaataccat agcgttatct
     2581 ggcacttgtt catctgctgc cataacttca aagtagtctg gtgaaacgat caataattgt
     2641 gcaatatctg catccaaagc ttttaacatt tcacgaactt gttgttttgg ataaacagtt
     2701 agttttgctt ccaaagattt accaatcaat ttgctgtttc ttgcttcttc caatgctttt
     2761 aagacattat cacggaagtc catgaaagca gtccaggtat ccatcaattc ttcttggttt
     2821 gcaaactctt cataaccagg aaactcgctt aactgaacgt actcctcttc ttctttcagg
     2881 aatgaccaga tttcttctgc tgtatgcgga atgataggag tcaataattt tgtcaaagct
     2941 acagctgttt gatagaaaac agtttgcatg caacgacgtt gataatcatc ttctgcttca
     3001 atataaacta catctttagc aaaatccagg tagaatgacg atagatcaac cgtcaagaag
     3061 ttcataactg tacggtaaat atgcatgaag ttatatttgt cgtacccttc ttctcggatt
     3121 tctttgatta cttggttcaa tcgaacggtc atatatttgt ctacagaacg taaatcattg
     3181 tacgaaacag tatctttttt aggatcgaaa tcttctgtat tcgctaataa gaaacgcatc
     3241 gtatttctga ttttacgata tacttctgcc acttgactta agatatccat agaaacacga
     3301 acatctgctt cataatctac actagatacc cataagcgaa ggatatctgc accaaattgt
     3361 ttgatcactt tttctggtgc aatgatgttt cctaaagatt tactcatctt acggccttca
     3421 ccatctaaag caaatccttg agaaataact gatttgtatg gtgctacacc attgatagcc
     3481 acactagtcg tgatacttga gttgaaccag ccacgatatt ggtctgatcc ttctaaatac
     3541 atatctgctg ggaatgataa ttctggtcgc tgacgtaaaa cagcttcatg agaagaacca
     3601 gaatcaaacc agacatccat gatatctttt tcttttgtaa attcgccatt aggagaacct
     3661 gggtgggtaa atccttcagg aagaagttct ttcgcttctt tttcaaacca gataattgat
     3721 ccatgttcag caaataattt tgccacatgt tcgattgttt ctggtgtgat gattgcttct
     3781 ccattttcag catagaaaat cggaagtggt actccccatg cacgctgacg ggaaattacc
     3841 caatcgccgc ggtcacggat catattatac aaacgtgttt taccccatgg gataacccac
     3901 tctacttttt ctacttcatc caaaatattc tgacggaatt tatcgataga tgcaaaccat
     3961 tgtggagttg cccgatagat cacaggcttt ttcgtccgcc aatcgtgagg ataactatga
     4021 gtaaagaaat ctagcttcag caaagctcct ttttctttta atagatcagt gatcatagga
     4081 tttgctttgt cataaaagat accttcaaat ccaggtgctt cttctgtaaa caatcctttt
     4141 gagtctacag gtgacactac agatagttta tatttgctac cagcgatata gtcatcttcc
     4201 ccatgtcctg gagcagtatg gactaatcca gtaccagcgt ctaaagtaac atgatcgcct
     4261 aaaatcacaa gtgattcacg ttcatagaat ggatgacttg cagtcatata ttccatttct
     4321 tgtccagcaa attcttttag aactgttaca ttttcccaac cgatttctga ttggactgtt
     4381 tctagcaaat ctttcgctac gacaaactta cggccatcgg cttccaccaa tacatacgta
     4441 aattttggat ttacagcaat accttggttt gctggcaatg tccatggtgt tgtcgtccaa
     4501 atgacaaaag ctgtatcttc atcaagaata tctttgccgt ctttgacatt gaaagctaca
     4561 aaaatagatg gagattttac atctttatat tcaatttcag cttccgctaa agaagactca
     4621 cttgacggag accagtaaat tggtttcaat cctttgtaaa tatagccttt ttcagccatt
     4681 ttaccaaata cgcgtacttg tgcagcttca taagaaggat ctaatgtaac ataaggatgt
     4741 tcccaatctc ctgcaactcc taggcgtttg aaatctgctc gttgcttatc tacttgggaa
     4801 agtgcatatt cacgacattt ttccaagtat tctgccatgg acatttcttt acgtttcacg
     4861 cctttattag ccaatacttg ttcaatcggc aatccatgtg tatcccatcc tggaacatat
     4921 ggtgcacgat agccagacat tgattttgaa cgaatgataa tgtctttact gattttattc
     4981 aatgaatgtc ctaaatggat attaccattg gcatatggag gtccatcatg aagaacaaag
     5041 ctaggttttc cttcgttcaa ctcttgtctt ttttggtaga ttcgattttc ttcccattct
     5101 ttttgccact cgccttctcg gtttggcaaa ttaccacgca tagggaattt tgtttttcct
     5161 aattgcaagg tttctttcat tttcattcaa ccatacactc ctttatttgt tttccggaca
     5221 caaaaaagca tgttcagccg caagggacga acatgccgtg gtaccaccca aatttagttg
     5281 catctatcta gtgcaaactc tctgtgacca ataacgaggt cagccgctgc ttcttactct
     5341 aatttcggaa aagcaggtct caagtagagg atctttcacc atgcagtgtc ttaccgaact
     5401 tccactctca tcggctcgct ttaagaataa caaagtgctt ttctgttcta ctcgttttca
     5461 ttttcaccag gcacgatcga gatatcaata gcctgtgtat caaatgccgt cataggtgct
     5521 tctatgactc ctgtgtctga gtttacatca gtatcatttt cattgtcaag ctgttttgct
     5581 aaaacttctt tgatcacttc atggctgtca gtcacgtagc tggagaatgg ttgtaaaatt
     5641 tcatcccatt ccggactttt cacttgttct aattgagact caagcatcaa gctgatacgt
     5701 tgatggaata ctcgtgtttt tttcttaaga tcttcagttt ctgtcgcaag ctgtcttgct
     5761 ttttctgttg catctgtcaa gatttttctt gctttttctt ctgcatcagt tgttaattga
     5821 tgtgcacgtt tttcagcgtt cgcaactaac tcatctgctt tgttttgtgc agaagtaacg
     5881 atcacttcgg attccttgct tgcacttgtt tttactttat cagccgtatc ttgagctacg
     5941 atgattgatt ggttcaaagc atcttttaat tcattgaagt actcaagttt ttcttcagaa
     6001 tgtttgactg ctttttctaa ttctcgattt ctttgagtca attcctcata gtcacgaaca
     6061 actaaatcta agaaatcatc tacttcgtct tgattatatc cccgcatttt tgtcgggaat
     6121 gtcttatttt ggatatctaa tggagttaaa gccatgagta aaccaccttt ttattttatt
     6181 tacgtaatac accgaacagt accctgtact tttctttttt cgtcttcccc tcaagttctt
     6241 ggatctggac tctgccataa cctctgactg aaataatatc taatagttca agtacaaaat
     6301 caggtcttgt attttctgtc cagttcactt ttactttacc agcttcgatc agctgcttgg
     6361 accgctgtct ggaaatatta taaatctcag agatcacact gtctagtcgc atggaactaa
     6421 ctgttcctct ttcttgtgtc cagctatctt tcggcataat catttctgtg tatttgattg
     6481 gttccaagcg tacagcaacc tttccaatct ttgttacttg tgcagcaaca aaatgatcca
     6541 cctcatttgc aataaatact tgccagcgat caccgtccga gatgatatcg ccaaagtaag
     6601 agcgtttgat ccccgttccc actaaagttc caaggatttt cccatgactt aggctggcaa
     6661 atttgctagg ataatggatt tcatacaatg ctgtttcaaa atcttcctct acaggttcgt
     6721 aataatcggg gaaaatcaaa catctttttc tttctgcctg ttcatatccg ccataaaaca
     6781 tgaaacgcaa atcactgctt tgtcgaatca gtgtttctaa aatataggat tgtcttgggt
     6841 caagaaattc ggtcagatag ggtgcatatt gtccttgaac ctgttcaatc caatcgccta
     6901 ccgaatcgat aaatgtacgt tcatctgccc gaaagtgctg gtatacattt gcatccaaaa
     6961 ttttcccctc ctatctattc tgagaataaa gattttgttg cttcaatcaa taaactaaat
     7021 ggacagttat ccatataacg atctgtgcta aaccttgtac ggctaagttc aagatcagga
     7081 ttgcccctaa aattgtgaaa tccaagggtc caatcctcaa aggcagacga tcgaacaaac
     7141 tgatatacgg ttcgcagatc ttagctaata atcgtcctag ccctgattga tacccgcctg
     7201 gaaaccaaga gagcaatgca tataccatca agataatgct atagaaatag gctgcccgtt
     7261 gaagtaaatg aagtaatagt gcaatagtat aaatcgtagt tcctcctctt acagatcaaa
     7321 caaattcgca tctgcaaaag attgagcggt tgaactgtca atctccatcc cagtcggtgt
     7381 acaaagaaaa atctcatctc caacacgttt gatatctcca tctagtgcat aaactgttcc
     7441 tgttagaaaa tcaacgattc ttctcgcttg tttttcttct actaaatgaa aattcactaa
     7501 ggttgcttct ccagcaatga ttcgtttagc aatcgccata gcttcagaat aagctcttgg
     7561 ttccattaca gtgatttttc cagcaagatt tgctttaggt tgtgtagagg ttcttgaagc
     7621 tgttctttct ttcgctacag tttttgtagg tgattgatgt agttcaatta cttttttctt
     7681 ttcagccggc tgaatacttt catgcgttac aggacgtgaa gtcgccgttt tttgtttttc
     7741 atgagtattc gcagcagata cagaatgagt tgattgattc tgtgttgtat tttttttagt
     7801 tggctgtaca gccttttttg ttgtttcagg ataatcttgg tattcgtact cttcctcatc
     7861 agctaatccg aaaaaattag caattgctcc tctgtttaaa agcggcattt ttgttcctcc
     7921 tttattcgtc tttgaatagc gctgttccta cacggataaa tgttgcacct tcttcaatcg
     7981 caatttgaaa gtcttgactc atccccatac ttaattcact acatggtgca tgggcataac
     8041 cttttgcttg aacaagatca cgcaattctt tcagcgtacc aaacacttta tggatttgct
     8101 catcagaagc ttctttaggc gccatagtca tcaacccaac aaccttgatt tttgtgcagg
     8161 cagctaaaga ttccacaaac ggtaaaagct catctggtga aattccatgt tttgattcct
     8221 caccagaaac atttacttca ataaaacagg ataattcttt ctctaaccgc ttctggattt
     8281 cttctgctaa tcttaaactg tccaacgcat gaaagtaatc aatttcattt acaatcaatt
     8341 ttacctttct gcgctgtaaa ttaccaatta aatgccactt tatttcagga aaatcaaata
     8401 aatcttgttt cttttctaat aatttatcta ctcggttttc tgcacaattc ataacgccag
     8461 ctctaaccaa ttcttcagct tgagcggatt caacagattt tgttacagct actaatgtaa
     8521 cttctttcgg atcacgatga acaagagcac acgaatcctg aatttcttgc ttgattttct
     8581 gcaagttgtc agcaatcatg tgctcttcct ctcctttatt ttttacgacg gaaaaacggt
     8641 ggcgtattta attcatcatc gccatgagat tttgtttctt cgcggttgaa tgtatcaaat
     8701 tctttttttt cgatattgtc aaaattggaa tcatcaactc taggacgaac attttgttca
     8761 cgtctgatat cccaatcgcc aaaactgttt tcttcttctg aagaagttgg tttagcttga
     8821 tccatatcta atacagtctt ttgagcaggt gattgcattt gtgcttgtct agctggtcgg
     8881 ctagacttgc gttccttttt tgattcatcg atacctgttg cgatgaccgt cacacggatt
     8941 tcatcgccca tttcttcatt gatagatgta cctagaatga tatttacatc gccagttgct
     9001 gcatttgcta cgatatctga agcatcttgt gcttcgaaca atgtcatatc caaaccgcct
     9061 gtgatgttaa gaagtacttg ttcagctcca tcaatagatg tttcaagcaa tggagaggag
     9121 attgcttttt tcgttgcttc tactacgcga tcttctccac tagccacacc gatccccatc
     9181 aatgctgtac cttggttttc catcactgtt ttcacgtcag caaagtcaag gttaacgtaa
     9241 cctggcgctg tgatcaaatc agagatacct tgtacacctt gtcgtaaaac attatctgct
     9301 tcacggaaag cttcaagcat cggtgtcttt ttatctacaa cttctaataa acggttattt
     9361 gagataatta ataatgtatc cacattttct ttcaaacgag cgataccttc tgcagcaaaa
     9421 cggccacgtt tcggtccttc aaaagtgaat ggacgtgtca cgacaccaac agtcaatgcg
     9481 cccaattctt tcgcgatacc tgcaacgatt ggtgcagcac ctgtaccggt tcctccaccc
     9541 attcctgcag tgatgaaaat catatctgca ccgtctaaag cttcgcgtaa ggattgttca
     9601 ctttcttctg ccgctttttg tccaacttct ggctgagaac cagcacctaa accacgagta
     9661 tatttggggc ctaattggat aactgtttca gcttttgaat tttttaatgc ttgcacgtct
     9721 gtgttggctg taatgaattc aacaccttta acgttttctt caatcattcg gtttacagca
     9781 tttccaccgc caccaccgac tccgatgact ttgattactg cgccgtcatt gatattatta
     9841 tcaattgaga attccatatc ttattcctcc caggatgaat taatcaaaaa tatttgtaaa
     9901 gaaacccttg attttatttt ttacattttc tccggactct tttggttccg gctcatcgta
     9961 gattgtttct tcaggtgctt catagctctc atatgtgttt gttgtttctt gttcaactac
    10021 catttgacga gctgctgagg tttcacccgt aacagcaatt tttgccaatt gatacacttc
    10081 actcaagttt gctgaataat ccacgatact tattacattt gtaaataccg ggtttctcag
    10141 ccccatgtga tttggaacat acaattttac atttacgccg aagatttctt gtgccaagtc
    10201 cacaacacca ggaagactag cagctccgcc tgtcaatacg acgccacctg gcaactctaa
    10261 tgcttcgatt tggtccaatg catcttttgc tttgttgaaa atctgttcca ttcgagctga
    10321 aataatttct gacaagtagc gttcatcgac tttcactggt tctgactgac caatcacatc
    10381 tactggaaat tcttcatctg gtgatgtccg ttctgggtat gcatccccgt agtttatttt
    10441 caacgcttct gcattgttaa atgacgtatt taatacaata gagatatctt tagtgacaaa
    10501 ttcgccacct tcttggtcaa gattggtaaa cttcaattgc ttatcatgca taacagccgt
    10561 tgttgtttgg ccgccaccca tatcaatgac gatcgtacca aaatcttttt ctccgtcaga
    10621 aagaattgat tcagtcagcg ctaaaggagt aatcaccatt tcgttgacga ttaatccggc
    10681 attttccacg catttacgta tgttatggat gatcgtttta ggtcctgtaa acaacagacc
    10741 gtacatttct aatcttacgc cgatcattcc acgaggatct ttgattccct cgaacccatc
    10801 gacagtaaaa tcttgtggca atattgacac gatttgacgt tcaggaggta ttgaacgaac
    10861 aagggcagct gaagccacat ttctgacatc ttcatcggtg atttcctttg aatctccgtt
    10921 aactgcaatc attccctggc aattttctac ttcaagcatg tttgctggca aaccgacact
    10981 tacgcctttg atctggatac ctgctttttc ttctgcttgt cgtactgctc gttgtatcgc
    11041 ttggaccgtt ttatcgatat caacgataat gcctcggtta atcccttctg attttgcgtt
    11101 tcctactcca ataatattca tttggctgtc gatatattca gccacgacaa ctttaacaga
    11161 tgtcgttcca atatcaaggc ctacatacat tcctgttttt gccatgaatg ggattccctc
    11221 ctacttacta ttccatatac aagcgaacaa ctagcgtccg ttttaaaata tacttattaa
    11281 tagatatatt cactgcttcc aattctacca taaattactg gtttttagaa agtacattct
    11341 tactttttaa agttgtttta tcaattttca tttaaacttg acggagttgt acttggatta
    11401 tcttctattt gacttgtttc acttgaatca tttgaatcag atgatccgtt tgtgctagac
    11461 gatccaggtg tttgtgcttt ttgcgtttct gcctcttcac ttgattcgtt tgtttgatcc
    11521 gcattttctt cactttcagt tgtttcttcc gattggcccg attcttccgt ttcttgagtg
    11581 ctaccgtaag gatgcgagaa gataccaact tccatgtcga tcacaccttt ttcatccata
    11641 tctttggcta cttgcgaata atatttcatt tggttcgcaa gattattgat atttacaata
    11701 acttgattgc cgtcattcat attcaattgg atcagatttt tattgcttgc tcttggcgtg
    11761 tacttgattt cagaaattgc tttttgtaat tctgatgata actgattgta ttgctttgtt
    11821 aattctttga tttttgcaga gtctttgaac tcttccaaga tagggagatt ttttgtagga
    11881 tttgctactt tttcatctaa tactgttcca ttttcaatca ctgggtagta ttgattgttt
    11941 tttgcgatca aagcaatctc tttatattct gttacatcca aatcgaattc attcatccct
    12001 ttgaaatgta tatttgctgt ttcgattctt ggctgtgctt ttttaaggcg atcgatataa
    12061 gtagatcgat gaaaatactg tccccaaaca ttttctccta atgacaattt tgtatcagag
    12121 atgatttctt ggctgttgac tgtcttattg cctgatactg caactgcttg taatttgcta
    12181 taaggagaaa cataatagat caaaaaaatc agcggtatac ccaatacagt gatgatcaaa
    12241 gctaaccgac gaaatagtac tttgtgccgt tgacttttta aattgggcag ccggttcaaa
    12301 aaagaaccgt tatatggtcc accgtttttc ttttgcttga tctcttcaaa aaattcgatt
    12361 tttttctcgg actggctttc ttcttgctgt tcctcttgaa ccgttttttc ttcttcagaa
    12421 tctgaaaggg gtgtatctaa tgttatgtct tctttttttg gctcttggtt tttcatctct
    12481 tgttctgcca gatattttcg gttagccttt tgccaaggag tcaaattgtt ttcatcagaa
    12541 gacggatgag acaatttttt tggttctttt ttcttactca ctaagaccac cgcctttatt
    12601 ttttgattat ttgttttact aagtcataaa gacgttttga agcatcaggg attccttgtt
    12661 ctttagaagc tcggcacatt tgcttttgca attcttcatc acccatgatt tcgttgactg
    12721 tctgacttag tgattcacct gttagttcat tatctgcaat catttttgct gctcctgcgt
    12781 gaacaagact cattgcattt ttagtctgat gatcattcgt aacatatggg ctgggaacca
    12841 acactgccgg taaccccaga gctgtgaatt ctgcgatcga agttgcacca gcacgcccca
    12901 ccagcagatc actggaagcc atgacctctg ccatattttt gatataaggc tggacactaa
    12961 tattttgtat ttcaggattt attttattta ggatctcttc ataatatcta tctcctgaag
    13021 catacaaaac ttggtaagaa gaaggaatat attcacttaa aaaaactgat actgcttgat
    13081 tgattttaag tgctccttga ctaccgccaa aaatcaagac tgttttcttt tctggatcca
    13141 aatcaaatgt tttcaaaata tctgattttt tagtatctgc tacttcttgt gctcgaggat
    13201 tacctacaag aactgctttg ttttcaggaa aaaaggaagc cgcatcttta aatgaaagag
    13261 cgatccgatc cacatatcgg cttaaaaatt tattggtaac acctggtaca ctgttttgtt
    13321 catggataac tgttggaata cccattttgg ctgcagcgta cactaccgct ccggaaacat
    13381 atcctccagt accgataacg atatctggtt gaaaatcact gaggattttt tttgcttgac
    13441 ggatactctt aagaaacaat cggaccgtct tgatgttatg catggaaatt tttcgtttaa
    13501 atccttgaat ctccaaggta tggaaaggca ttcctgtatc agggacgatt ttgttttcta
    13561 gacctcgttt tgctcccaca tacatgaatt cagcattcgg ttcttgagtc tttacataat
    13621 tgacaaaggc aagagctgga taaatatgtc cccctgttcc tccgcctgta accaaaattt
    13681 tcatttttta tcgctcgctt ctactttttc tattttaatc ggttcacagc ttccatgaat
    13741 ctgtttcctc tagtttcaaa atttgggtat tgatcccagc tggcattcgc tggtgataat
    13801 aaaactacat caccatctgt actgttttct aatgctaaag gaactgcttg ttctacagaa
    13861 tcggctgtta ctattgtctc gatgcctgct ttttttccag catccatcaa cttttctttt
    13921 gtttggccaa atacgaccat tgctttgatt ccttttaatg aaggaattaa ttcatcaaat
    13981 gtatttcctc tgtctaatcc gccagccaat aacacgacct tagacgtttc aaatccactt
    14041 agagccattt tagtcgctaa aatattggtt gcttttgaat cattataaaa ttttctgcct
    14101 tggatttctc ctacatattg cgtacgatgt ggtacgccat ggaaaaaatg caacgtttct
    14161 cgtatagctt catttgatat accatacaat tttgctacag aaatagcagc tagtgcattt
    14221 tctacattat gcttacctgg tacgccaagt tcagatattt ccattatttt ttctttttta
    14281 tagtatattg agtaatcgtc agcatacaca ccgtcttcca acacttcttt tgtcgagaaa
    14341 ggcagtacgc gtgcttttgt tctttgcgct aattcttgta gttctgactg attccagtta
    14401 aggatcagat aatctttttc tgtcatgttt ttttgaaggt tccatttagc tttgacatat
    14461 tcttttcttg taccatgata atcaatatgt gcttcataga tattggtaat caccgctata
    14521 tgagggcgga aatctgtgat tcccatgagt tggaagctag acaattccat cacaatttta
    14581 tcttctgatt ttgcttcttg cgctacacca cttgctggat acccaatatt acccgctaaa
    14641 cgtgcaattc cttgatctgc tcctgcattc agtagtagtc ctgtcatcgt tgttgtcgtt
    14701 gtttttccgt tcgttcctgt aatcccaata attgggcatt ccgctacttc gtaagctagc
    14761 tcaacttccg taattaccgg aatacccatt tcttgggctt ttgctactaa aggatgactg
    14821 tagggaatcc ctggattttt gaccatcaat gaaaattctt catccaacaa ttcgattgga
    14881 tgactacctg ttattacttt tatccctaaa gaaagcagct cttgtgcttc cggattttcg
    14941 tcaaaaggtt ttccatcatt aacggtgaca agtgcgccca attcatgtaa taatttggcc
    15001 gcacttactc cgctttttgc gagtcctaag acaagcactt tcttattttc atatgttgat
    15061 atttttttca tttcccgtca tctcctacca aacgatccat aatgtgatca acgaagtcac
    15121 aatgctgatc aaccaaaaaa ctgtatcaat cttccactcc gaccatccgc acatttcaaa
    15181 gtgatgatga attggagaca ttttaaaaat tcgttttcct gtaagcttaa aggaagtaac
    15241 ttgaagcatc acacttgctg tttccataac atagatcaaa ccaatcaaaa gcaatgtcca
    15301 ttcttggttt agcattatag aaattgccgc taaaagtccg cccaacgcta atgaaccgac
    15361 atctcccata aaaatcttgg caggttttct gttgtagacg aagaaaccca gtaaacctcc
    15421 taaaacactt aagcaaatga ttaatacatc atactgatgc tgatgccaag caattagacc
    15481 gtaagctgca aaagagatac ttcctaagcc agcaactaat ccatcgatgc catctgtcag
    15541 atttactgca tttgaaaatc caacaagcca gaagagagcg aaaataccat aaatgattcc
    15601 taaaggtaga tctagtccga agaaattcaa tacacctgga taaccttcag aacggtaaac
    15661 aagataaaag atgattcccc ctagaatctg tccaatcaat ttctgcttcg agttcagtcc
    15721 catgtttctt ttcttgaata tttttataaa atcatcaaga aagccgatta ctccgtataa
    15781 cgctaataca aaaagcaaga tgaaaagagt aggagttagc tgtttttgcc aagctccaac
    15841 ccaaattcct gtcaaaatag agccgatcag aaacactaag ccgcccattg taggagttcc
    15901 ggctttgcta ttgtgccact ttggtccctc ctcacgaatc gcctgacctt gctttttcat
    15961 ttgaaaataa ccaataaata gcggcattgc ggcaattgtc attgcacaac tgctgacaat
    16021 gggaatcaat gcttgtgtcc attccatgct cttttctctc ctatttctct tcttattctt
    16081 ctaaagtaaa actaagtttg tcttcagtga ttttttcata tggagcaagt tcttgtttga
    16141 cacaataacc atctccatca aaactaactt caataccgag gattttcccc agcttcatga
    16201 tatctgcttt tgaccatcct gttacgtcag gcatcagctt atcaccacca gtatatagga
    16261 tcagtttttc tccagagatc aactgatcac cgttggcagt agattgtttt tgtacttttt
    16321 taccattacc gatcactaca ggttgcagtc cacttttttg agcatcagct gctgcaacgt
    16381 ccgcttcaag attacgataa tcggctacgg atactttttc tgtttttgtt tctgtatctg
    16441 aatcttcttc agtttcttta aaatccattg cccgtttcat caatggattt gcgattttag
    16501 ccaatgccat tcgatcatat gtcttagggt gtttcattgt tagatacaag acatagtctg
    16561 gatcttcaga aggaaccatc tccacaatcg aatacaggta ggctgtatct cctgtttgat
    16621 atccccctgt atcagaagcg atttgggctg ttccgttttt tgccgataca ttgtatccag
    16681 gtacactata tacaccataa gcactaccgt agttttcgct ttcgacaaca tctctcatat
    16741 attcacggac tttttctgtt gtttcttttg aaaaaggttg ccctagaact tctgtttgag
    16801 ttgttcgctc ttctccagtt tggggatcca ccactttgct gatatatcga ggttgaatca
    16861 ttgtaccgtt attcgcaata gaagtgaaag ctttcatcat ttggaaattc gtcacaccta
    16921 ccgcttgtcc ataagcactc attgctcggt cgacaatatt taatgtaggt aaagcaccgt
    16981 ttacttcatc atctaatcca gaatgagtac tttgtccaaa tcccagtttt tgtaaatagt
    17041 tataccatcg tccgcctaat cgctgttcca gtatgaccat cccgacgtta cttgaccatg
    17101 aaagagcttg acgcatggtc agcacacctt tttcaccaaa gtcatggtca ttgatcgtag
    17161 catcttctac ttggattttt ccagattgga aggtttcatt ctcgttgaat tctccttctt
    17221 cgatagcagc tgcagtagtg aaaactttca tggttgatcc tggttcataa ctatcttgta
    17281 ccaagaaatt acgccagata gcatcttttc cagtcaatcc ttccattgtt tcaggattga
    17341 aagtaggtcg ttgtcccatt gccaagattt ctccggtttt tgctttcatc aggactgctg
    17401 tcaattcttc tggctgatat tcttcattta cttgatccat cagtgtttct aaataacttt
    17461 gtagtctgct gtctaaagtc gtgtagatat cttgtccgtc gacagctttt tcttcttctg
    17521 ccactgtacc tggcagagga ttttgaaaat tatctttttg ataaataatc ttaccatctt
    17581 taccgctcaa gatatcattg taagcagact ccaaacctag tttaccaact aagccattct
    17641 catctttgtc tggaactgcg tatccaataa aatgagaaga aaagacgcca tttggataca
    17701 tccgagcctg atggtcgaca aaataaagac cagcgacacc ttcttttttc atgtccgctt
    17761 cgatgttttg cttggtttct tgggagatat ttttcccata gctaccaaat tcaacttgat
    17821 acagctcttt ttttgcgccg tcctctaaaa tattcagtgc atcttttttg tctatagaaa
    17881 gattcttatg caggatttct gctattttat caaaattctt ttcttcaaca tacagttttt
    17941 tatccccaga ggtatatgtt tttgagagta tcgctttaat tgaataagag gaggcatctt
    18001 ctgcgagcgc cacaccgtta cgatcataaa tcgttcctct ttttgctttt actacttcac
    18061 ttccttggta caactgtttt gttttttcag ctaatgaggt accagcaaca tgacctccaa
    18121 taacgatata cgagaaacgg acggcaaata aaaagaacaa tccaatactc gtagcaaata
    18181 aaatgatacc tacttttttg cgattgttca tcggatttag atttttcttt tccataaacc
    18241 gccgaatttt atttttaaga ctcatttact tcactttcct taaattgtcg tcattaatag
    18301 acaatccttt ttcttctgcg atttgtttaa tccgttcagt acgagaaagt tcgttttttt
    18361 cttgttctaa actcgttttt tcttgctttt tatcattgat ttctgtttgt attgacgtga
    18421 tttctttttc agctccgctg atatttgtgc gcagcataac tgttaaaaca cataaagcaa
    18481 caatagcaat cgccacgctt gccacagcaa ttttttccaa agcagaaatc tgctttaatc
    18541 gttttgccgg tgattgtgga agaatgattt cttgattcga agaagaatca ggcatttgtt
    18601 gttccggctg atccaccaat tcaggtaaat catattcgta atcttgcatt ttctttaatt
    18661 ctgccatcat tctactcctc tttcattctt attttttctg caatacgtaa ctttgcactg
    18721 cgtgatcgat tgttttctgt tagctcttcc tctgaaggaa ggattggttt acgagtaatg
    18781 actttcagtt ctggttggaa ttcgtcaggt atgacaggaa gtcccggtgg tagatcttgt
    18841 accgtactat attctttaaa catacttttg acaattctgt cttccaatga atggaatgtg
    18901 attacgctga ttcttccatt gactttcaat aaccggattg cctgttctaa agaggcctct
    18961 tcggcaccga gttcgtcatt tactgcaatc cgcacagctt gaaaaattct ttttgcagga
    19021 tgcccgcctt ttcttcttgc gggagctggg atgacttgtt taatgatttc gacaagctca
    19081 ccagttgttt ggattggttt tgtttttcgt actcgttcga tttcacgagc aatttgtttg
    19141 gaaaatttct cctcgccata gcggaagaaa atctttacta attcatggta ggaatattca
    19201 ttaattacgt gataagcgga taaaggagct gactgatcca tgcgcatatc caacggtgca
    19261 tcttgatgat aactaaatcc acgttctgct tcatctagct gaggagagga aacccctaga
    19321 tcatacaaaa tgccgtctac cttatctaca tgctcactca agcgttcttc taattctctg
    19381 aagttcgctt tgataaatgt gaccatacct tgttcgacat aagaagctaa acgcagcttg
    19441 gcgtgatcga ttgctttttg atcttggtca aacgcataca gatgcccttt agaacttagt
    19501 tggcttaata aatattcact atgtccggca ccgccgagtg tacagtctac ataaacgcca
    19561 tccggtttga tgttcaatcc atctaccgtt tctttcaaca tgactgtata gtgttgaaat
    19621 gtttcagtca ttagtcagcc tcttcctatt cttatagtcc gaaatcaatc atactttcag
    19681 caatttcgtc aaagttctct tctgctgctg cagaaaaatc ttgccatcgt gtctcatccc
    19741 aaatttcaat tcggttagat acaccgatga ctacgcaacc ttttgtcaaa gctgcataat
    19801 ttcttaacgt gctgggtata ttgattcgtc cttgtttatc aagttcgcat tcagtggcag
    19861 ccgaatagaa gaatcgcaca aaagtacgtg cgtctttttt agccaacggc atttcgttca
    19921 gtttagcttc cagattttcc cattcgctca tcggatagcc gaaaagacag ccatccaatc
    19981 cgcgtgtcag aacaaacttt tcgccaagtt cttcacgaag ttttgagggc acgattaaac
    20041 gtccttttgc atcaatatta tgttggaatt cacccatcaa cattgaaagc cccccattcc
    20101 tccactaact aaaattagtc taccacaatc ccccactttc taccaccttt tctgggaaat
    20161 ccgggaacaa ctttttttat tcataaacat ttttttaaaa atcaagctgc aaaaagcctt
    20221 ttaaaagact ttttacagct tttctatttt tttcataggt aagaaagtat gttcacaatg
    20281 atcaagacag cgtacaagac gattgtcaac agaaaaacca aacgccaagt catcttgaaa
    20341 taacgcggat aaataatttc ttcataataa tatgcttgga aaacagcaat acaaattccc
    20401 attagcaaaa cagaaataaa gaaaaacggt aagatagaaa ttgtccctgt atttctagac
    20461 aatgcatgca taccgcccca caaaaaagga atcgccaaat caggggcttt tattttaaaa
    20521 cgacgattta acgaaaataa ttttacaaga aattggcaac caaacaaaac gattaccggg
    20581 taaatatacc aaaacaagat aagtggtgtg aatgtgccca tgaacgtaaa ctcctttttt
    20641 atcgttgctt caagtatact gtatatactt tacacaactg tttcatttta tttaaattta
    20701 tgcagttata ttctatatct tacaaacaca ttccatgtat tccaattccc tatccgttat
    20761 aataaaagaa acaggcaaca ggaggaaaat catgatcaaa tattttacat tagataatga
    20821 gaaactgcgt aataccggaa aggaaactga ccaaacgatc tggtattgtg tagaacgtcc
    20881 taatcaagaa gagattcatc agctgacaca tcaatttgaa attccgtcag attacatcac
    20941 ttccatattg gacgatgcag aaaattcccg tacagaagaa ttcaatcaat caaagttgac
    21001 aaagccagcg ttattattgt tacaatatcc ttttgctaaa acaagtccta gcggttattt
    21061 tcaagtggat acatacccgc tctctattat tttgacgccc aaaaagaagc ttatcacaat
    21121 tacgaatcat gaaccactgt ttttaaagaa agtctttagc cagacctttc caaaaagtga
    21181 tatttcgcct aatctcaata tcttctttca attcatttgg caggtcacac tctcttacaa
    21241 cagttattta gctacgcttc aatctcaatg cgatactttg gaagccaaac ttcaagtatc
    21301 cactgaaaac aaacaacttt atcaaatcat ggatatccaa aaaagcctcg tttatttcag
    21361 agaagcgact tctgctaatt tagatgcatt gaaagaacta gcgcaaaata aacttttcca
    21421 agaaaatcat gctttgaaaa atcatttacg tgatgtattg gtggaaacag agcaggcaat
    21481 gactacttct cgcattcaat taaaactagt agaacaaatg aatcagactt tttcagcaat
    21541 cgtatcgaat aatctgaata atattatgaa aattttaact tctttaacga ttattttaac
    21601 tataccgaca atcgtcggca gtatctatgg aatgaacatt aagttaccta ttgctgaaag
    21661 ggatgatgcc tttatatggc tagttctatt aacattattt atttgtatcg taactaccta
    21721 ttatttaaaa aaagggaagt ttttataaaa aagccctctt gtggaaatgg aaaaaagatt
    21781 gtacacttga atcaactaat taatgacgag gttgaaaaca atgcgtgaaa aaaaacaaac
    21841 aagtactttt gctaaagtaa caaaatttgt catttggaca atgctgattt taacgattgg
    21901 atctgttgta ttgactgctg taatgagtgt catgtaaaaa taaaatatag ctatcagctg
    21961 ctgaaaagga cctgaaccac tgcgaggtgg ctcaggtcct ttttgcgagt ttcaatagtt
    22021 cttttgcatg ttcgagtgtg agttttgtaa tctcacttcc agccagcata cgtgcaattt
    22081 cttcttctct ttcttttgtt ttcaactctg aaacagaagt ttgcgtacgt ccatcaatca
    22141 ctgctttcac aatataatat tgatagtcgg cgactgcagc aacttgaggc agatgagtga
    22201 tgcacaaaac ttgagagtat ttcgatattt gagaaatttt atctgcgatg gcctgtgcaa
    22261 ctcttccgct cacacctgta tccacttcat caaaaataat acttgtcaca ccttgctcag
    22321 aagaaaaaat agttttcaaa gcaagcaaca tccgagacag ttcgccacca gaagcgactt
    22381 tcaccaaagg cttcagtggc tctcctggat tggtcgtaat gtaaaattcg atttggtcaa
    22441 atccatcatg attcaattgt ctattttctt cttttaagaa acgtacctca aactcagtgt
    22501 tttccatata cagacttttc aattcatgca gaatggattt ttccaattct tttgcgattt
    22561 ttttacgcgc ttcatgaaga tctgcggctt gttgctgaag tagaagctct ttttgctcta
    22621 gttcctcttt cattttgtcc aattgacctt ctgaaaaatc agaagaagct agctcttcgg
    22681 tgatttcgtc atagtaagcc aatattgact caatggattc gccgtattta cgtttcaact
    22741 gtcggattaa ttctaaacgt tgcgtaactt cttctaaacg actctcatct aactccaata
    22801 aatcaatctg acgactcata tcgccgattg catcttgcag caaatagtaa gcactttgga
    22861 tattttcata aattgcttca tactccacat ctagatgggc gatcccttgc agttctgaaa
    22921 cggccaagcc aacaccatct acactgtttt gttcattatt cgtcagtgct ccgtaacctg
    22981 cagctaatcc atcagcaatt ttttgataat tgctaagttt ttctctttct tcttttaatt
    23041 tctcttcttc tccgacttgc aaatcagcag cagctatttc ttcttgctgg aaatggagca
    23101 tatctattct ctgaacatag gatttttcat tttgctgcac ttttcgtacc cttttttcca
    23161 gctcacgata agagatatat tcttgctgat aactcttttt cttctgttgg aaagctacat
    23221 ctccaaaacg atccaataaa gcaagatgtg attcaggctg gagcagttcc tgatgttcgt
    23281 tttgtccttg gatatctact aaataactgc cgattcttct cagattagct aatgtgataa
    23341 tatgaccatt cactcgacaa acattttttc cagttaagga catatcccgt cgaacaatca
    23401 gattgtcctc atctgtttcg atccctaatt caaccatcag ttcagagaat ccttcttgtt
    23461 ttggtagttc aaacaaccct tcaagcacac atttttctgc tccttgacgt atataatcgc
    23521 ttgaacctct tcctcctgct aacagaccta aagcatcgat aataatggat ttacccgcac
    23581 cagtctctcc ggtcagcgcg gtcattcctt cgtgaaaaga aagccgcaat tccggaataa
    23641 tcgcaaagtt agtaatactg atttcttgta gcatcacatt cgctcctcaa cttaaaggta
    23701 atgcaggaag tctttcttca ataaaactgc ctggtcttca gttcgtgtaa tcatcaaaac
    23761 attatcgtca tcattgataa tcgaaaataa ttctttcttg taacgtttat caatcaaatt
    23821 tgctgctgcc gaagcatttc ccggcaatgt tttcaaaata acaaattttt ccatttggtc
    23881 aactgccacg aaagcgtctt ttaatagttt ttccaattta gtatttacat cttcgtttgt
    23941 ttcagctggt atgctataac ggtagccgcc atctgaagcc ggtaccttga tcagtttcag
    24001 ttcctttata tctctggaaa tcgtagcttg agtaacagat attcctttgc ttttcaggat
    24061 ttcaacaaat tcttcctgct ttcggatatc ttgctcactc aacaatcgag taatcaaacg
    24121 atgccgatct ttttttctca ttacgtcttc gctccttagc atattcttct taatcaatga
    24181 tagcttattt ccttggtata ataaagaaga atccgcatac ttctcacttg attttcacag
    24241 ttacaattta tttatggtca aactgttcgt gtgcatttga aaccacttct gccatattaa
    24301 tagctgaatt gattgttccg ctttcgggta ctttcctcaa gtgagccaag aactcgatat
    24361 ttccttctcc ccccgtgatc ggagaataac ttaattccag cacatcatat ccaccttgtg
    24421 ccgcaaatga caaaatgtct tctaaaactt tttgatgggt ctgagggtca cgaacaattc
    24481 cgtttttccc tacagcttct ctgcctgctt cgaattgggg tttgattaaa gcaatcactt
    24541 ctccctcttc ttttaaaata gcatgtaatg gaggcagcat cagttttagt gaaataaaag
    24601 aaacatcgat aacagcaact tctggaattc ctaaggtaaa gtcttcgggt tttgcataac
    24661 gaaaattcac tctttccatt acgacgactc taggatcttg tcttattttc caagctagct
    24721 gattgtagcc aacgtctaaa gcataactca tttttgcgcc attttgcaaa gctgcatcag
    24781 taaatccgcc agtggatgca ccaatatcta acaatgtttt tccttcaaca gatagatcaa
    24841 atgctttgag tgctttttct aattttaatc cccctcgaga aacataagga agttttttac
    24901 ctttgactct taattcacta ctcactggaa ttttttctcc cggtttatca aaccgctcgt
    24961 ttttttcgtt gtaaatcaaa ccagccatca ctgaacgttt ggcttgttct cttgtttcaa
    25021 ataatccttg tttgaccgct aatacatcta cgcgttcttt ttccattctt tccctcattt
    25081 ctccacatca aattgcttga tcatctggcg catcagcccg ccatcaaaag aaaaaacgtc
    25141 ctcttctaat ttgtctatga tcttgttggc ggataacagc tcaatctcca acgcctcacg
    25201 tgtcttttct aggcctaata aacgcgggta agtatttttt tccatacgtt catctttacc
    25261 agctgttttc cctaatgttt tcgttgtacc aatcacatcg agtagatcat ctcttatctg
    25321 aaaagctaaa cctaaatgtt cggctaatcg ttgaagtagc aataagattt cttcaggttg
    25381 cttcgctaat atgccaccag ccagcaaggc ataacgaatc aaatgccctg tctttctttc
    25441 atgaataaat gctaattctt ctaaagaaag ctttttcgat tcgccctcga tatctgcagc
    25501 ttgcccagca accatacctt gtgatcccgc acaaacagct aattgctgaa gcagcagaag
    25561 cttaggagaa ttacctaagt gtgccatact gattagctgg aatgctcctg taagcaaacc
    25621 atctccggcc aatatagcta atgcttcgcc gaatacttta tgattggtag gttttcctct
    25681 acgaagatca tcattgtcca tcgcaggcaa atcatcgtgg atcaaggaat acgtatgcac
    25741 catttctaaa gcagctgcta cttgatagac gggaacatca ataggctcat caaaggaagc
    25801 aacggccgtt aaaagtaata agggacgtat tcttttcccg cctgctttca ctgaataact
    25861 catcgcatca tagagtcctc tatccgttgt atgctcatct aaaaaagaca agatttcttt
    25921 ttctacagca ggtagatgaa tagctgaaaa gtcagttaat ctcatctcag ctttcctcgt
    25981 ttccgtcaaa atcaacttct tcatttgatt ctgtcatcat tttcgtcaag gtctcttctg
    26041 ctttagctaa tgtatcttgg caatgtttgc ttaattccat tcctcgttta aacgaatcta
    26101 gagctgattc taatggaacg tctccttgtt ctaactgcat gacaattttt tctaattctt
    26161 gcagtgactc ttcaaaagtt ggattttcca tatttttatt ctcctttgtc tatctcttgt
    26221 acttgtcctt taacgattcc atcttgataa tggatcgtca gcaaatcccc ttgatgaatc
    26281 tcggtgacac tttttactac ttgatcattt aacgtcgtat agctgtagcc tcttcccatg
    26341 attttcaatg gactcaacaa atcaagagat tgaacagctt gagcaaaacg ctgctgctgt
    26401 tgtttcatat attgttggat tttctcttct aagcgttttt ctagataagc agtttcttgg
    26461 cgagctgctt taactcgata aagcggagcc acttgttcca attggtggct taatgcgatc
    26521 gtttgttttt ctttcgcatg ataaatttgc tgtgtagctt gatataatcg ctgtttcaac
    26581 tggtctaatt tgatggtctg accttcatac aatctttcag gctgccgaaa tacataggaa
    26641 cgtcgagcac gctcgaaccg ttcttgtttg cgctggatct ggcgtacaaa cgcctgctgc
    26701 aatctagctt gccgttcttg aatgcgcatc aactcttctg acaaaacagg aacagctaat
    26761 tctgcagctg ctgttggagt ggcagcccga acatctgcta ctaaatcagc gatcgtggta
    26821 tctgtttcat gtcctacaga tgaaatcacc ggcgtattag cttgataaat cgcccgagca
    26881 acccgctctt cattaaacgg ccataaatcc tcaatcgaac cacccccacg accgatgatc
    26941 attgtatcga aattgcccat ctcttctact ctctggatat tcctgacaac attgtccgct
    27001 gcttggtttc cttgtacaac tgttgggaac aaaaccaatt ggacaattgg atatcttctt
    27061 ttcaccgtcg tgatgatatc gcggataacc gctccgcttg gactggtaag gaccgcgatt
    27121 cgctttggga aacgtggcag cattttttta ggcccttgaa aaaggccttc cttcatcagc
    27181 ttttcttttc gttcttccaa ttcttgatat aaagcaccta ttccatcagg ctccatgtgc
    27241 tctacataga tttgataaga acctcccgct tcatataatg aaatacgccc aacgacaagg
    27301 actttcattc cttcttttgg ctggaatcgt aatttttgga atgctccttt gaacataata
    27361 gcagatattt ttgcatggtc atcttttaag ctgaaatatt gatgggcatt ggcacgtaaa
    27421 cggaaattcg atatttcgcc tgttaaatat acgcgttcta aataaggatc ggcatcaaat
    27481 tttcttttca aatacttcgt taatgtagtg acagttaaat attcttgtgc catctattca
    27541 cctgctccaa aagcttcggc agattttacg gtttgataca tcagcatcgt gatagtcatt
    27601 ggccctacac cctttggcac aggagtgatc cagcctgcaa taggctccac ttcatcaaat
    27661 ttcacatcac cgatcagctt accgttagca tcacgattca tccctacatc tatcactaca
    27721 gcacctggtt ttacgaattc ttttgtgaca aaatgtccac gtcctattgc tacgactaaa
    27781 atatctgctt cttgtgccag ttcagccaga ttttcggttt tagaatgagc aacagtaaca
    27841 gttgcatctg ccatcaagag tagctgagcc atcggtttac cgacgatatt gcttcgtccg
    27901 ataactactg ctcgtttacc cgccggatcg atagaataag cttcaaacat tttcatgatg
    27961 ccgtatggag tacaaggaat cttgtccggt ttacccgcaa ggagtctgcc taaattcatc
    28021 ggatgaaaac cgtctacgtc tttttcaggg cggatagcta acaggatctt ttcttcatct
    28081 atatgctttg gcaatggtaa ttggaccaat ataccatgaa aactaggatc ttgattgtat
    28141 ttttcaattt ctgcaagtag ttcgtcttca gaaatcgtct caggataacg ctctaccaat
    28201 gaatggattc cgatttcttt tgctacacgt tctttatttc ttacatagat ttggctggct
    28261 ggattttccc ctaccaataa aacgactaat ccgggatgga taccattatc tttcaatttt
    28321 tggatttttt cagcaatctc tgcctgcatt ttttcagcta attcttttcc gttaatcaat
    28381 tctgccatct aaccactccc agatattttt tgtgttttca ttctaccata gaattttccc
    28441 gatttataac aggtttatga tgattcatag aaaaaagcta ggacttacgc cccagcttct
    28501 aattctttca atacattgga taaaacaccg ttgacgaatt tacgtgaacg gtcatcacta
    28561 aatgtttttg ccaactcgat tgcttcattt aaagcaacag ccgctggtac atctgtcaca
    28621 taagtcattt caaaaatcgc gatacgcaag atcaccaaat ccatttttga gatccgattg
    28681 atgtgccaat tgttttttaa atgtttttca atcactttat ccaattcatc tttttttgca
    28741 cagacaccgc caactagcgt atcaagataa acaggaacaa actctgattc atcttcgttg
    28801 atcatgtcgc ggtggtctaa ttcgattgcg ttgaaaatcg catcttcttt tgttaggtct
    28861 gcattgaagt ctaatggaaa taaagcctgc aatgccattt cccgaatctc gtgtcttgat
    28921 aattctttat tcattctctt cctcttcgtc ttcaaaaagt tcttcgattg ttggttgttc
    28981 taatttttca ggaacaacag ctactacatg gatattgact tcagataaat cgatatccgt
    29041 catgaaaagg acttgctggc tgacccgttc ctgcatttcc attgctactt taggaactga
    29101 aacaccatat tctagataac agtaaagatc tactttcaag ccatcttcct cgtttgctaa
    29161 gtaaacacct tttccgtgag ctgcacgtcc taataattca gtgacattgc tggcaaaagt
    29221 tccgcgcatt ccgtatacgc cttctacttt agaagctgca atcccgataa ttacttcgat
    29281 gacttcagga gcgatcacga tctctcctaa atcttggttt gcgtttaaaa tcaaattttt
    29341 ttcatctgcc atttatttat tcctcccttt gcctaagcac gagatacatc cgaattctct
    29401 atgatgcggc atagtgatca cgtggtaaga aaactgccgc cgatcagctg caaactgcta
    29461 tgaattggag aaaacaccca actttattat ctcttacagc ttaaaagttc ttctatagtg
    29521 tatcattaaa tcgagtgatt ttctataaca aagtctgtga acactgattt ttacgcttac
    29581 ttcttctatt gatgaaacta taagatgatc aattcttttg gtgaacgagt caacacacga
    29641 tttccatctt ctgtgatcaa caagtcatcc tcaatacgta ctccaccgat tcctggtaag
    29701 tagatccctg gttcatcagt gatcacattt cctggtacaa attgcttttc tgcacgagaa
    29761 gaaacgtttg gtccttcgtg gatctctaaa ccgatcccat gtcctgtaga atgtccaaat
    29821 gcttccccgt aaccatgttt tgaaatataa tctctagcaa ctgcatctaa ttggacacca
    29881 gtcattcctg gttttgcagc atcaataact gctaattgag catctaagac tatttgataa
    29941 atttctttta atttttcccc aggatcacca atagcgaatg tacgtgtcat atcggaaacg
    30001 tacccttcgt aatagcaacc aaagtccaat gtgatcaagt caccttgttc gatgattttt
    30061 ttgcttgcta caccatgcgg catagccgaa cgcaatccgc tagcaacgat cgtatcaaaa
    30121 gaaacacctg aagcgccaag agaacgcata taaaagtcta gttgatttgc gacttcaatc
    30181 tcagtcatgc ccggttggat cattttcagg atgtgatcat atgccatatc cgcaatatga
    30241 catgcttttt cgataatagc gatttcttct tcatctttca cttctctgag ttcttcgatc
    30301 atctgtgaaa caggtactaa ttccgcatcg atgatttctt ctaaaaccga atattctaaa
    30361 aatgagactg ttgtttcctc aaaagctaat gagtgcagtt cttctttgcg tactaagttt
    30421 gctacctcat caaaaatagg tccgacattt ttaataattt caaacccttg agcctgcgct
    30481 gccgcctgct cggtataacg aaaatcagta ataaaaaatg ctttttccaa agtaataaca
    30541 gctaatcctg tcgtacctgt aaagttcgtt aagtaacgaa gattgtacgg actggtaacc
    30601 aaaaatgaat ccaaattttc ttcttgcatt tttttgcgca atttttctac acgtagcatc
    30661 ataatgaaac tccctctttc tttgcacgct tttttccatt atagcaaact tttcttcgta
    30721 cacgacagtt aaataaaaat ttccggaaaa cttcttttta ttttaaataa ataaagccaa
    30781 aaaggagtca cagtctcgct gcaactcctt ttagtttact attttgtaaa aacttatgat
    30841 cgttttccaa agaaaaatga gacaactgct accaaaatca ctgcgcctaa aatagatgga
    30901 ataagtgcca tacctgcagc ttttggtccc cattcgccga aaatcgcttg tccaatactt
    30961 gaaccgacta gtccagcaat gatattcgtt acacagccca tcgaatttcc tttactggtg
    31021 atcgcacctg caattgctcc gatcacggct ccaacaatca atgtccataa ccaactcata
    31081 atataacctc cttctttgct aatcaatttt agcatatggg atttaaaggg gacaaataat
    31141 tggttttgct atttcatatt ttcttctgca aagtaaaaaa ataaagaaaa cctcctctta
    31201 gaagttttct ttatttttta cttgcgttta aacatactac gaataccacg tttcactaca
    31261 tcaaagaaac tcaatgattg taccattcta tcttcatcta catattctct gatcgcacga
    31321 agcacttttt taggttcttt ggaagaaaat gtgtatgtac catttttttt cgtttgaatg
    31381 gcatatcgag gaatccattt tccctttagc atgactgatg ctagtacata atccacttct
    31441 tcccatggaa tttggatata tttgcgtcta tctcgcgcat ggtaaaactc aaaaccatta
    31501 ttacctatca tgatttgccc gtaatctgtc agacccgtaa acgaagtagc atcaattacc
    31561 aagtctactt ttgtattgat tgattgaacc attttctccc tccagatatt tgtttatgaa
    31621 cagtatacta tataaaaaaa gctcgggaca acgcccgagc tatattttta tggattataa
    31681 aagtccgatg acgtgtccaa caacaccgac tacgaataaa ccaaggatga taacgattgg
    31741 agaaaccttt ttcttaagca accacataca taacaatgtt aatcctaatg cagctaaacc
    31801 aggaatcaat tggtctaagt tgtcttgtaa tgttgttacc ttttcaggag aaagggctaa
    31861 gccattatta acttgttcaa atgcactctt gattccttca ccacctgaag gcaatttatc
    31921 ccattcgatg taagcacctt tatctaattt tacagttgat acaactggta agaatttgat
    31981 tgatacccat ctttgtacaa gggcagctaa tacgaacatc cctaaaattg aagcaccttt
    32041 tgttacgtct tgtaacaatc cgccagaaag gtcatcagta attttagaac cagctttgta
    32101 gccaaattct tgtgtatacc acatgaatgc ccaacgaata atgttccaac ctaagaagaa
    32161 gatgattggt ccaaggatat ttcctcccat agctaatgaa gcacctagtg cacctagcat
    32221 tggacgtact gtaaaccaga acactgggtc accaacacca gctaaaggac ccatcatacc
    32281 tactttaacc ccttgaatcg ctacatcatc aacaggcgcg ccattcgcac gttcttcttc
    32341 cagagccaat gtaaccccta aaattggaga agcaatgtat gggtgtgtat taaagaattc
    32401 caagtgacgt tttaatgctg cagaacgttc ttcttttgtt ttgtataatt ttttgattgc
    32461 tggaatcata gaaaatgccc agccaccgtt ttgcatacgt tcgtagttcc atgaaccttg
    32521 aatgaaggta gagcgccacg caacagctaa acgatctttt tttgataatt tgattttttc
    32581 ttctgccatt ttattttcct ccttcaagat taatagtcat tcaaaatgtc gcctaaaggg
    32641 tcaccagagt tgcttccgcc tccattattt gatgaaccac ccattttaga aaggttcaag
    32701 taaataagag ctaatgcaac acctaaagca cctagagcaa tcaatgttaa ttgagaaatt
    32761 gcagctacaa cgaaaccaat aacgaagaat ggccatactt cttttgtagc catcatgttg
    32821 attaccaaag cgtaacctac agctacgacc attcccccac cgatagccat accatctgtt
    32881 aaccatgctg gcattgattc aaggaatgat tgcacagttt gtgctgggat aaatagcaac
    32941 gctgctgctg ggaccgcgat acgaacccct tgtaaacata cagcaatgat gtgccacatt
    33001 tcaaccttac ggatgtttcc ttcttcagca gccgcatcca tcaaatgaac gattggaaca
    33061 gcgattgtac gtacgatcat tgttaagaac aaccctgcaa cagccaaagg aacggcaatc
    33121 gcgatcgcag aaggaacacc tttaacacct tgtccaccta aaactaaaat aattgctgat
    33181 gctactgatg ctaatgcagc atctggtgct acagcggcac cgatatttgc ccaacctaaa
    33241 gcgatcatct gaagcgtacc gccaagtacg ataccagctt cgaggttacc ggtaactaaa
    33301 ccgatcaatg tacatgccac tagtggttga tgaaattgaa attcatcaag aataccttcc
    33361 ataccagcta gaaaggcaac taatacgact aaaattattg aaataataga catgataagc
    33421 ctcctataaa ttttaatatt atttattacg cttttgctaa ttcagctttt gcttttttca
    33481 aaatatcatc catgttatcg cgtgaatcat taggtacttt acgtacatcg aatttcacac
    33541 ctttttgttc aagcttctca aacgcttcaa cgtcttcttg tcccatagac aatactttac
    33601 ttacaacgac tttaccgacc gaatgcgcca ttgacccaac atttaattct ttgatgtcta
    33661 cgccaccttc aacagctgtc aatacgtctt caggattttc aaataaaagc aatgctttgg
    33721 tatttccaaa acgagggtct tttgctactt cgatcatttt gctaatagga ataacatttg
    33781 cttttacacc tggaggagca gcttgttcga ttagtctctt acgcagatca tcctttgata
    33841 ctgcatctga aacaacgata atacggtttg gttgcactgc ttttgtccaa gctgtcgcaa
    33901 cttgtccatg taacagacgt gagtcaacac gtgctaatac atatttgatt tttccgtctc
    33961 cgaccacagt tccttctgga agtgctcctt taggttgtgc gtcttcaact gctgctttcg
    34021 gtgcttctgc cggttctaat tcttccggtt ttacttttac accgtctttt gctgtttcaa
    34081 tgatgtgtgc tgcaatttca tgagcagatt ccattgaaaa gcgtgatgcg taggcttcaa
    34141 tcaacattgg taaattcaaa ccgctgacga ttgcccattt atctttgtgc tcttcgaata
    34201 atgtgtttga ttgattgaac ggtgttccgc cccataaatc aactaagaat aacacttcgt
    34261 cttgattatc aaaagaagca accgcttctt ccaatttggc tctcaagtca tctggacctt
    34321 cacttggttt caaaatcacg gctttgacgt tttcttgttc gccaaagatc attgaaccgg
    34381 attgtaagat tccttcagca aattgtccat gacttgctag gataatccct accatattta
    34441 tacctcctat ttacttttgt tgtaacaaat tcaaaaatat taataattgc taaccattac
    34501 aacagaatta tttgaccttt tccaacaacc attcttgcta cacctttttg attttcgcta
    34561 gtagttccat caaaaggatt ttcctgtcag caggtacttt tcgaatctct aactccactt
    34621 cttgactgtt caagtaacga aaggaatcaa tatcttgttg atttactgaa acaaaattgg
    34681 taatcattga tttgccttca gtaaaactca taccgccgat attcaacgac ttcaactgta
    34741 caccgcagcg gacaaccgct tcaacatctt ggggattggt gaataaaagc atcacttttg
    34801 ctcgtgataa taatccacta tgatagattt gaatcatttt ctgcacggtt attacatttg
    34861 ctgtgatccc cggcggtgca gcttctttta gtagaaactt tctcaattta tcttgagcga
    34921 ctacatcgct gatcacaatg atacgttcaa cacctgtttg cttcgaccaa actgttgcga
    34981 cttgcccgtg gattaaacga tcatctatac gtgccaatcg aatatccatc tgcaaaactc
    35041 cttcttcctc actacttcac agaaacccct atcatttctg tgtgaacaac cacctcctag
    35101 cacacactac tataagcaaa aagcgtgcca acttttttac gatacactaa ttgaatttaa
    35161 aggattctta accaatacac aaaaacgata cactataaaa gtgtatcgtt tttgtgtatt
    35221 catggcacag tgtattcttt tttatctaac tgattatgga ctagacgtag cagataatac
    35281 atttcttcta tcggcacttt tacttttatc tgatcttcca acagaacaac tgaggctgcc
    35341 aattggctat aaagaggatc attcgttgta tttgtcaatt cgttttcttc cacagtcaat
    35401 ggttcattcc gaataatacg ttcgatcatt cctgctgtgt gcatcactag gttgattttg
    35461 aatccatatt cttcatcgcc gattcccgtc gtctgagaga tttgtgtaga aaattgccac
    35521 attggatcga ttaacttact accattaata aagatgaagt tatcagagat aaactcaaca
    35581 cacgtatttt tagctgactc ttcatctagg gagatgaatt cagtttcctc caattctgat
    35641 tccatcagca actgatccaa aatgacttcg atattttgat caataaaacg ttctaaagga
    35701 ataaatgggg cttggatatt caaatcagta atcccagtag tagctatgat ctgatattct
    35761 ttttggataa ctggcagttc ttctttcact tccacaatcg acaaagctag aacttctaat
    35821 tctgtttctt gacgcttgtt taccgctcgt tcgatcaatt ctttgattct ttgtgctgta
    35881 ccttcgccag atgcgcagat agccaaaaca gcttttttct tatgaatagg ttttgcttcc
    35941 tgaacagatt ctacagaaga tatttctgcg taacctcgaa aatttttcaa tgattcatgc
    36001 aacatatcta gatctgtccc taatactgag acctttcgta cagtttccaa aactagagaa
    36061 gttgttacca tatcaacagt acgtactgga attccagtgt ctctttgaat ttgcgaatta
    36121 aacgacgcaa gggaccccat atctactaag agtatgaccc cgcttccttc atctacttct
    36181 tgtacattgc gttcaattcg gtccaatgct gttttcggat ccatatccaa aggcatgtct
    36241 actgctcgta cgttctctgc atctaacaac tgttggacaa cttggaccat actgcttgct
    36301 gtgctgtttc cgtgagctgc aacgactaca ccgattctgc cgcttgcttg atttgcccgt
    36361 aacgagacga gtagaacggt caagtaatag tcttcacttt ctggaatctc cacttgaaaa
    36421 tacgtttcga tataactacg cacttctttc gcaacagcat attcatctgg aaaatcaaaa
    36481 gccatctcac ggatgttatc attcgtatgc cgttcttctc ctaagttgat ttttttcaaa
    36541 aaagaactga tatgcaagct catagcataa ataaagtttt gctgaaactc atatggtaaa
    36601 gatcttttga ccattacata aatctgattg gtcacttcta tgattttcgg atctacaaat
    36661 tctgctaatt tgctgtctgc attaaaagaa aaaccatggt ctttatagaa agacttcaga
    36721 tggatgttga tatctgtaga aatgaaatga ttgattgctt cttgatccaa accatccgac
    36781 ttcagtaaag ctgctttatc tccgatgata tcatatagat tataaggtaa ctcataggag
    36841 tccgattgga ttttcatgat cgtatcattt ggtgctacag ttatctttgg ctccaatatt
    36901 ttagaaagct ctgccattgc actgcggttg cttgctaatt gaatcaatcc gctacgaatc
    36961 ccttctgtca aatcatcgat tgtgatcgag atttcgggtg attgcatatg atttagaaat
    37021 cctcgtgcac atactaactg gatatttgac ttcaattgtc ctatatttcc ataggtcaca
    37081 ctgccgatca atgcttttgc tacatcttct gtcaaagaaa tctttcgctg cgtgcggttt
    37141 gcttcgtggg caatcatgat ttttactaaa tcgactttct catttgcagg acgtttttca
    37201 aaagccggaa gtttgatatt gattgggata cgccgaacaa aagtttctaa tagagaagaa
    37261 cctgggtctt ctgtggtcgc acctacgatc cgtacatttg cttcatggga tttcgttgtt
    37321 tccccaagac gactgtaagt tccatgatcc ataaagtaaa agatcatttc ttgaccttca
    37381 ggcggcaacc ggtgaatctc atccaaaaac agcataccgc catccgcttg atcgataatc
    37441 cccatttttt cttcatctgc gcctgtaaaa gcaccttttg cgtacccaaa taaatgactc
    37501 atcaaaagct cagggttatt ggcataatca gcacaattga aaacaatcag ttccgtttcc
    37561 tcatctatta catgatgcgc ttttgcaaaa tgatacatcg cgtgtgcaaa ataagtcttt
    37621 cctgatccgg taggtcctgt aatcaaacaa tttaatcctc ttggcggata caaaatcgca
    37681 gcttttgcct gttccactga atttttcatg ctgccgttag ctccaatgat ttttgcaaag
    37741 atatccttag tatctatttg gagtgtattt ttttcttcag agaccggcgt ttctttgtaa
    37801 ctgactacat gttttgtaac tggctttacg tgcgtaacga gtgtttgata tatgtaacgt
    37861 actggtcgtc cgtctgtctt tatcagtttt ccttcgcgca ctagctgatt cagatcttta
    37921 ctgctatttg ttcgctgaat ccctaacact tcagcaattt cctgtgttgt tactccttga
    37981 tcatattctg cctttgttaa atgagcggtt ttttctttga catacatata aattcggtca
    38041 atgcgttttg ccaatggtat cgctcctttt ctatagacta ttgtacttag gcttcaaaac
    38101 ataatactat gtatttattt ctgtttcgtc aactatttac ctgattttgt tttgttgata
    38161 acacgcggat agatagaaat gttcaattta aaagaacgaa aaatcccgat acgattttta
    38221 taatggaaac aagaaaaaga acactgttta ccatataatt atttcactga tagcaaacaa
    38281 tatagcttcc atcaattgaa aacgatagtt atcaaaacat cctgattcca aattggcaaa
    38341 aaaatattaa gtgtaaaaag acaaagtaaa cttcgtagcc tccactacaa cttttatttt
    38401 tataaacaaa aaaggctgtg acaaaagtct tgtcacaacc tctttaatca taagattcaa
    38461 ttaagcttct tgagctactg gataaacaga cacttgtttt ttgttacggc ctttacgttc
    38521 gaaacgtact acgccatcaa cttttgcaaa taaagtgtcg tctccaccga tacccacgtt
    38581 tacacctgga tagatttttg taccgcgttg acggtaaaga attgatccac ctgtaactgt
    38641 ttgtccatca gcacgtttag cacctagacg ttttgattct gagtcacggc cgttagatgt
    38701 agaaccgcca ccttttttgt gggcaaataa ttgtagattc atagataaca acatggtctg
    38761 cacctcctat ttatcattaa ttgttttaat ttggatatat tctgagtttt cagcttctat
    38821 cgattgtaat cctaataaaa gattttctaa tagaatctca gcaatattgt tttgttcttg
    38881 tgtcattcct gaagtgactt cggtataaag ataaccgcct tcttcttcat tcatttcaac
    38941 aatcggctca aaaccggcta atgaggcaat cccgttgaca gtgctgatcg ctaacgcaga
    39001 aacacccgca caaacgatat cacttccata aggaccagca tcagcatgcc cggtcaaagt
    39061 gaatgaaacg atctgacctg cgtcatttcg cttaaatgtt cctttgatca tatataggcc
    39121 ttctttctca aacgctatta agcgttgatc gcgtcgataa ccacttttgt atatggttga
    39181 cgatgacctt gtttacggtg tgaatgtttt ttaggtttgt atttgaaagt aacgactttc
    39241 ttttgtttgc cgtgtttttc aacagtacct tcaacagttg cacctgcaac agttggagcg
    39301 cctacttttg tagattctcc gcctactaag ataacttcgt caaaaacaac tttttctcct
    39361 gcttcaacgt caagtttttc aacatagatt gcttgtccca cttcaacttt tacttgttta
    39421 ccaccagttt taataattgc gtacatgcta taaagcacct ccttctgata tacttagact
    39481 cgccatccga agtgacagca tatgctgtgc ttattacttg ttctgagcgg ttgtagctgt
    39541 ggagtccaca attacaacag tactagctta ccagaacagc aaccgttagt caactaaatt
    39601 taaaatcaaa aagtaaaaca aaaattcaag caatacttct gcttttctat cctcacagtt
    39661 ctaccaaata aaatagtcgc attgtgtttc ttttattctt ataataaaag aaaaaggagg
    39721 agcataaatg gaatacccaa aagaactgat acaagaagct tctttaagaa tgaacggccg
    39781 tcctgtagaa ggatttatcg ctgggcaagg acctcttcat cctaaactga tgctcgtggg
    39841 agaagcccca gggaaaacag agattgatac gcatgttcct tttagcggtc aagctggaaa
    39901 agaattgatg caggcacttt cttcaactgg cttaacacga gaagaggtgt atatcaccag
    39961 tgctgtaagg agccgcccgt atcgcgtgac tcatcggatc aataaacgga cacaacagtc
    40021 agaaactgtt tatccaaatc ggacacctac tcgttccgaa gtatttgcgc atgcaccaat
    40081 attggattac gaactgatga aggtccagcc aaaattaatt gcgacacttg gaaacattgg
    40141 tcttcaacgt ttacttggca aagaatatac ggttaccaaa aatcatggtc aactctatac
    40201 agggcctgtc ttacaattaa cagaacaaaa ggatgcctac gaatggtcag aaaaaaacta
    40261 tcgaattctt ccactttttc atccagcagc tattttctac aatcgctccc ttgtacctgt
    40321 tatccatcaa gactggcaag tactagcttc acttttacag caatattaaa aagaacagac
    40381 aagaccataa aatcttcagt ttacagtctt gtctgttctt tttcattgtt ttaatggcta
    40441 attgcagtat atttcgcatg taaagctgtc aacgcatgag caactgtttg atgttgatgc
    40501 gtgaatgtat gttcccacaa gtgagaaagt tgtttttcta cttctggctg caaggaagta
    40561 tagataatcg tttgtcctct ttgctctgct ttttcttgaa attcatcaaa aactgcacgt
    40621 gccgtcaaat caatacttgg tacgcctcgc atggagaaaa tgacaccatc gtgatgattt
    40681 agttcttcca atttattttt gagtttttct gaagacataa aaaacagtgg accactaata
    40741 tacactactg tccagttatt cagcttttcc gtttctggca gccccattct ttgccaatcg
    40801 atcgtttcta atgaaatcgt gatagcagcg cttttgacaa caaaaaatac acagccgccg
    40861 atcaccccga tcacgatagc aatacttaaa tcaaagataa ctgtacaaat catcgtaagg
    40921 ataaacaata gaactgctga ccaatattta ttagtaaata gctccttgat cgtttcccat
    40981 tcattcatac gccaagccgt cacaatcaaa acacctgcaa gtgctggcat tggaatctgt
    41041 gacatcactg gcgcaaaaac aagcatggag agcagtaaga caactgcatg tatcatacca
    41101 gctaatctag tttgtgcacc agatttgatg gccacgcttg tacgggcgat ggccgcagta
    41161 gcaggaatcc cgccaaagaa aggaagcaac agatttccaa ttccttgcgc cattaattct
    41221 tgattgctat ctaagggctt atttgccatt cggccagccg aagcaccaca tagcaagctt
    41281 tcaatcattc ctaataaagc aatgctaata gctggaactg ccacttgttg caaggcagaa
    41341 aagctaaaat ctcccatatt caaacgatta ctactgatca atgtttgtgg aatcttaccc
    41401 acagttgcta ctggtaaatg gaacaatagc ataactgctg ttgccaaaat gatagccagc
    41461 agcgaactag gcattcgttt cgcccatttt tttggataga taaacatccc taaaatgact
    41521 agccctccca tgatcaaaga ggctgtgttt atcggaaatc ctatcgtttg ataactactt
    41581 agtttttcaa taacgtttgt accttccgag tgaacaccaa ataaattatc gatctgacct
    41641 aatgcaataa tgatagcaat acctgaagta aaccccgtaa ttacgggtgc tgggataaaa
    41701 gaagtcagtg ttcctaatcg gaaaagtcct gctgcaagta aaaatagacc cgctaaaaaa
    41761 gtagcaagca atacaccttg cattcccttt gttgccgcaa tagacatcaa gatagctgcc
    41821 atcgcacctg tcggacctga tatttgataa aaaccgcctg ataaaccgcc aataacgata
    41881 ccggcaatga tagccgtaat caaacctgcg gccgcatcag ctccgcttga taccccaaaa
    41941 gccaaggcta aaggcaatgc tacagcagct actgtgatcc cagccaaaag atctttctga
    42001 aatcttgaaa gatcataacc tgaaaattct ttttttagtg aggaaacata atttttgaac
    42061 acctatctaa ctcctttttc atgaaatttt ctaaaatacc attcattaga ata
//
LOCUS       DAZKLM010000015        38134 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3764
            SAMEA115706913-rid22582563.denovo.015, whole genome shotgun
            sequence.
ACCESSION   DAZKLM010000015 DAZKLM010000000
VERSION     DAZKLM010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706913
            Sequence Read Archive: ERR13196647
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 38134)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38134)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287823.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287823.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,004
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,849
            CDSs (with protein)               :: 2,849
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 41 of 96
            Pseudo Genes (incomplete)         :: 52 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 15 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38134
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706913-rid22582563.denovo.015"
                     /isolate="VRE3764"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            159..839
                     /locus_tag="ACG842_000732"
     CDS             159..839
                     /locus_tag="ACG842_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HFQ0247294.1"
                     /translation="MKKNMIGTLLLSTLLLGGLATPAFAEGQATSKGDITFTEPTNTV
                     EPLNPTDPSKPVEPADPENPATGQTGPLTLDVVPELPFGTHEIESGTKTYQVDASKND
                     TPYLQVSDRRGVGADGQAQGWNVTVSVSDFVNGSQVLQGAELDFGTSTVKSTSDNEST
                     GPTSQTVTGLSKASAATPIFTAAKDQGLGTWLSVYDPANITLKVPKAAAGTFTADLTW
                     NLVAGPVA"
     gene            929..1963
                     /locus_tag="ACG842_000733"
     CDS             929..1963
                     /locus_tag="ACG842_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HFQ0247295.1"
                     /translation="MNQSKRLFVSFFSILSLFFIFPSISQAKDSAGDFGIKPVFPENQ
                     IDKAIGYFDLLVAPEQNQTLEVIISNSSDEERTFEVSVNPAVTSDGGTIDYSQKNPTL
                     DETLPFDVRDVLLIAKKEINVSAHAETTVPIEVKIPAKSFKGRVLAGIHVSPKEEAET
                     ENATEGAQIKNRIAYNLAVVLQESQETIEPDLKLLSGDLDEVNAKPTVQLRFQNPQPR
                     IISNLIFTSKIFYENQLYIENTSNAFLVAPNSNFHLNLDLAGDKAKAGDYRAEIIAKS
                     GDSNEWRFTQNFTIKKEKAQKVNENSVFAVQEQSFPWLYAGLVAAVLVLWLILLFLWV
                     HKKKKKGETT"
     gene            1977..2312
                     /locus_tag="ACG842_000734"
     CDS             1977..2312
                     /locus_tag="ACG842_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0247296.1"
                     /translation="MKTSILLGGIIASLLFFSWQEPVQADTKSSGTITFYDLSPIPHP
                     SGSVNGASKQQTQLPIYKESNQHGVFPETNEQKIRFLYLIIGIELWLLAWLLLWRRKE
                     KEDEHVATK"
     gene            2296..4776
                     /locus_tag="ACG842_000735"
     CDS             2296..4776
                     /locus_tag="ACG842_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0247297.1"
                     /translation="MLLQNKFSFFRSFFFTSLFVLSIFIFSPSAEAVGATPPAISYDP
                     YTYENIVPPNGGRSAVNGGKLVQLTGANQLPKLTATYLNDGSNDPWKGGTVRFHWESH
                     VKLTNVLDLVNITAQGFAFVAIINLPEHVSSSDVLKAIDWNNAYLYIAGAPIKVTSGS
                     LLSAGSHTLRFGMGSWDTNTLLTDIIAIITRGEFNVDDIPLQFDVTANVAKMTENGDK
                     YDTSPNKILTKGKLQPSMSKKAVFSVDFYDSNNIIQDRMFNSILAPAAGRFYPKLTGG
                     NLNPGAYAFSASAEINTWNSYISPSDSTGKYNTFADTTEYVDGNNQNLPGSTNSRTLT
                     MGLTEGRFDQLPFSRFDRVVNYFTGKNVTQGAILTHTPASTSGLNQTANIVYSGKDAQ
                     GGLLSPVALKVLEQYKLDGTVLPTNLTADSQWGKLLSSASYQVTANWSATELKKGSIH
                     YRLYSKGTQKLATGWTDQLFQTIQSNNGGVNTATSTLPALASGQYYFDYRLVDDVLTN
                     MYPSFAYKWQSEQAGITTLPQITVANFPSVSADSQLKNLSRQPETGDPLSALSGDTIQ
                     ENVTFTLTKSGDAITDKKITISLPENTTYINGSLKLNGTTIADTGIQQGVSVPADLLS
                     KIGDTIHLTYNYQLNTVDTSLQSVSILTKAAVLSSNITLADGAKLPNPVVQTSAKTIL
                     VPKQELTLVNVPDDFTFGNDLPKPLKTSYYESKGDFSFDVRDTRLPSTSPWQLTGTLT
                     SLFKNDQGQELSGTKLYFNHSGSKQLIQQGQNTLIYESDGTAKGEVLVDFPDTDGLLL
                     EVNSSTNAQPGATYQGMVTWELTAGPTS"
     gene            4832..6391
                     /locus_tag="ACG842_000736"
     CDS             4832..6391
                     /locus_tag="ACG842_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0247298.1"
                     /translation="MRRKLLLLKSEMLEGVDLELFSLLEKNEQRQIKLFQLLLEEKGE
                     VSVNNVLTRLSVDRATLKEDLFYLQANLENIKQLEIVIQEGNILLIRHGNIAAVDVYY
                     HCYLRYSTKYQILLYLLENGSFDRQKLVMELNISSATLSRRVKELNDSLNEFHLKIKS
                     GRLTGPESQISYFYFQLFWFGRPYLENKKEYSDLSGNTFLDLLQKEFSFAITEEGRLK
                     FEIWLSIMKKRMRCTKKSKPDYSNLPVGLFQTTQFLLDLHTFLTRYFFQYAFPWNQNE
                     TLVFYLFMISNFTLDTHNSHVQEFLISEKYKDDVVAQLNRTFKQSIEEYFSFYVFPEE
                     ISQKIALTVMQNHAHFAYFRGWMNIFGQEGLKQRLTEVEDKQFLVLCERLVRQTFAIV
                     DFTEVDEENARFELFARYASILRTLIKQLELRLQIAYDLPYERLMADIVKEIIQEQLL
                     NTIKYDLVEYEKEKQYDIILTSQLKEYPSQKSAKVFVFTSNEYNYDFPYLNQFLKECY
                     LEKLNLRMNKT"
     gene            6388..7752
                     /locus_tag="ACG842_000737"
     CDS             6388..7752
                     /locus_tag="ACG842_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding oxidoreductase"
                     /protein_id="HFQ0247299.1"
                     /translation="MKIVIIGASHEGITAALNLRKLQPKAEVLLIDEDHKDGLGYVSN
                     GINLYLKGKIRSLAEVAHNMRTLQESGAKLITEWRVTELDPDKHQLILASKEGKNETI
                     TYDKLIVATGSSPVTLYKQIEAENVYTYKNLVQSKQVLAALKEAKEVVIFGAGYIGLE
                     LADALRNKGYIIHLVDYMPNVLSRYFDKDMINSFQNQLQTKQINFYPNEFLIDWKKSE
                     EKVVSVQLLSQTIKADMVIFSAQTRPNTTLLKEKVALYEDETVIVNEYLQTSDPDIYA
                     IGDIVPVSFDKNKRHLFLPLVTRAVHMARAVALTLSGQPTAYDLRQKITAAVIIDYFL
                     GTVGLTEDEAPFLEQNTGSCSGEFDLFPQYDEENKTVNAKLIYHPDTLEIIGGQLISQ
                     EFLLSDLNLLADIVKHKTTIPQLAVEDFGFLSEYTSRFHYLNELAFKVLAKRANSNRV
                     DKRL"
     gene            7923..8993
                     /locus_tag="ACG842_000738"
     CDS             7923..8993
                     /locus_tag="ACG842_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0247300.1"
                     /translation="MFLALNEIMHSKLRYALVAGVMFLIAYLVFFLTGLAYGLAQDNR
                     TAVDKWEADSIVLSKDANSNLGMSMITKKIAEEVEGGKVAYLAQTPGVVTSKDSTEEG
                     KINVSFFGIDKNQFIMPNLVEGKAFDNDDEAVGDISLKEEYGLAIGDTVKLSGSDKTF
                     KLTGFTDHAKFNVSPVLYTTINAYQEIRFEKEDTSENARINAIVVRGKISNLPEDLEQ
                     IKISKFINELPGYNAQVLTFGFMIGFLIVIAAIVIGIFIYVLTMQKINIFGVMKAQGI
                     TGGFIARSVVAQTFILSFVGILLGLLGTVGTSLVLPDAVPFQSNWLFFGVISLLMLVV
                     AVLGALFSVRTIVKIDPLKAIG"
     gene            9008..9676
                     /locus_tag="ACG842_000739"
     CDS             9008..9676
                     /locus_tag="ACG842_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0247301.1"
                     /translation="MKAIEFKEVEKSFQDGDQVVKALKETTMSIDKGEFVAIIGPSGS
                     GKSTFLTLAGGLQTPSSGSVFINDQEFSNQKESERVKLRFKEIGFILQASNLVPFLTV
                     KKQLLLVDKIKHEHRQKEANELFEQLGVDKLINKYPEELSGGERQRVAIARALYNDPS
                     IILADEPTASLDSEKAREVVDILAKESKDKNKATIMVTHDTRLIDKCDRIFMMEDGVL
                     KEKE"
     gene            9696..10274
                     /locus_tag="ACG842_000740"
     CDS             9696..10274
                     /locus_tag="ACG842_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFQ0247302.1"
                     /translation="MVHRYNKDTQQKILKEATDLFMSKGYLGTSTREIAQKVGITQPN
                     LYHYFGDKEKLYTAVLENHLKDVGKALREIVQTSETGFQVTLTKMAQFLIETHLVDLF
                     LMLHDLESNLSKETRDHLFFLWKKNYREPFEEIFSANQSVLRNGISKEIAARHFFLVL
                     APYITRSKRPVEEPLNVAQLIDLYLNGVLGEA"
     gene            10675..11586
                     /locus_tag="ACG842_000741"
     CDS             10675..11586
                     /locus_tag="ACG842_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HFQ0247303.1"
                     /translation="MKKIIGAFVFAIIIIIICFQIDVFGQKKIVGQTVSSTNSTVTKT
                     KESFDDTEQETLIKIGKNDRTLYTDDSLTTPLATINGGELAEFLSETKNAYQIKTNDG
                     YTGYLALEDGTKVTKNIQTKPEELSDAVIVLDPGHGGNDTGALSNDEQTEEKDITLST
                     AIKVKKALEDAGATVYLTHTTDELVQLGDICDYSEEKKADVFISLHADSTEYANEATG
                     ITTYYYYGQEETLAQTIADSFTDLPLDSRGISTGNYQVLRENLQPSILIEMGYMNNDS
                     DLKELVTDSYQQQIAASLTQALTTYFQ"
     gene            complement(11964..12881)
                     /gene="rnhC"
                     /locus_tag="ACG842_000742"
     CDS             complement(11964..12881)
                     /gene="rnhC"
                     /locus_tag="ACG842_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HFQ0247304.1"
                     /translation="MSNVIIKATNDQLSQMKAYYRSYLLNKQIPYTSFAAKKDGTTIT
                     AYTSGKVMFQGTNAAIEADKWGEAQDKQAKPAKTASSLPKNISQLSVLGSDEVGNGSY
                     FGPLTVCAAYVKRDQLAELRKLGVRDSKELKDSQIIQLADILKKTIPYKLLVLSPEKY
                     NEIQPKYNAVRMKVALHNQAIHLLLKKIEPEKPEAILIDQFTPEANFKKYARLEKNQL
                     QEKLYFVTKGEQYHLAVAAASIISRASFLEELDKASAEAGITLPSGAGTKSDQVAAKL
                     LEKGGMPMLEKYAKLHFANTEKALKLLKK"
     gene            13083..13520
                     /gene="zapA"
                     /locus_tag="ACG842_000743"
     CDS             13083..13520
                     /gene="zapA"
                     /locus_tag="ACG842_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HFQ0247305.1"
                     /translation="MAHEKTRYKAVIANQTYTIIGRETKHHMDIVTKLINEQLAELKQ
                     LSPQMDNEQAAILMAVNALSDQLKKQERILELEEETAELKKKMIKFTELENRVKRIEA
                     IENEAREVLKENGQADHTIKNHVEAQQILNEKRKGQIKQKSSN"
     gene            13578..14126
                     /locus_tag="ACG842_000744"
     CDS             13578..14126
                     /locus_tag="ACG842_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009403 - toxin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HFQ0247306.1"
                     /translation="MLSLLILFLLLIAFFSGARRGFALQIIFAIGYVLSFIAAQHFYK
                     PLASHLELYIPYPAVTPTSKLAFFDQVFAFHLDEAFYAGTAFLIILLIGWLLTRFVGV
                     FVHGLTYVPILRQVDWIAGGILSLIMAYVTIFLILQLLAFVPLDVVQNQFGEHSFARF
                     IVERTPFLTNKMYELWVTNILN"
     gene            14241..16601
                     /locus_tag="ACG842_000745"
     CDS             14241..16601
                     /locus_tag="ACG842_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HFQ0247307.1"
                     /translation="MNNRILETLEFEKVKQMVRQFVVTAQGKEELAELVPVSEKQTIT
                     NWLQETEDGLKVQRLRGGIPIPKIENIRPHMKRIEIGADLNGVELAQVSRVLSTTSEL
                     NRFIDDLSDSEIEFARLYMWADQLVTIPVLSRRLKEAIDEDGRVTDDASPELKSIRQN
                     IRRSEQAVREQLDGIVRGKNAKYLSDAIITMRNDRYVIPVKQEYRGVFGGVVHDQSAS
                     GQTLFIEPKQVVDLNNRLRQYQIAERNEIQRILSELSAELVPHRQEIIHNTYVIGKMD
                     LMNAKARFGKEVKAIVPGISEDNHVVLKQARHPLIDQEKVVSNDITIGKDYQAIVITG
                     PNTGGKTITLKTLGLLQLMGQAGLPIPAGEESQIGIFEEVFADIGDEQSIEQSLSTFS
                     SHMTNTVDILSKVNEKSLVLFDELGAGTDPQEGAALAIAILDDLGKKSAYVMATTHYP
                     ELKVYGYNRANTINASMEFDVDTLSPTYRLLIGVPGRSNAFEISSRLGLDTEVIDEAK
                     QLMNDESQDLNEMITDLENRRKMAETEYLEMRHFVSEAQELHDDLKEAYSYFFEEREK
                     EMEKAKKKANEVVSEAEEKAEKIIADIRKMQQQIGQGNVKEHQLIDAKTQLANLHQEE
                     TLKKNKVLKKAKEQKTLKPGDEVLVTTYGQRGTLLRKNGNQWQVEIGILKMNVSEDEL
                     TPVAPQKEPTQRVIHAVRSESSSHVPNQLDLRGKRYEEALSEVDQYLDSAILAGYPQV
                     TIVHGKGTGALRKGITDYLKNHRSVKSFEFAPANQGGNGATIVKFK"
     gene            16688..17002
                     /gene="trxA"
                     /locus_tag="ACG842_000746"
     CDS             16688..17002
                     /gene="trxA"
                     /locus_tag="ACG842_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HFQ0247308.1"
                     /translation="MSQVITDKTFNEETDKGLVLIDFWATWCGPCRMQAPILDQLEQE
                     YDEEEFRIAKMDVDENPETPQQFGIMSIPTLMLKKDGQVVEKAVGVHSKEQLRQMIDQ
                     YL"
     gene            17184..18962
                     /gene="uvrC"
                     /locus_tag="ACG842_000747"
     CDS             17184..18962
                     /gene="uvrC"
                     /locus_tag="ACG842_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HFQ0247309.1"
                     /translation="MNERIKNKLALLPDQPGCYLMKDKNGTIIYVGKAKILKNRVRSY
                     FTGSHNTKTERLVSEIEDFEYIVTESNIEALLLEINLIKKNDPKYNIMLKDDKTYPFL
                     KITNEKYPRLVITRKVLKDKALYFGPYPDVGAANETKKILDRLFPLRKCKPSQTKEPC
                     LYYHLGQCLCPYYFDVDPKVYTKIVDEVKNFLNGGHEKIESEIHQKMEKAAENMEFEK
                     AAEYRDQIRAIETIMTCQKMTNTDLLDRDVFGYAIDKGWMCVQVFFVRQGKLIERDVS
                     MFPFYNEAEEDFLTYLGQFYQENEHFIPKEVLIPDTIDKPSVEALLATKVLQPKRGEK
                     KKLVELASKNASVALNERFDLIARKQERTIGAVERLGNAMNIPTPIRIESFDNSNIMG
                     TNPVAAMVVFIDGKPARKEYRKYKIKTVNGPDDYASMREVIYRRYSRVIREGLPLPDL
                     ILIDGGKGQVDVAKDVLENQLGIDIPIAGMVKNDKHKTNELLFGTDLAVVPLERNSPE
                     FFLLQRIQDEVHRFAITFHRQLRSKNSFASRLDEIEGLGPKRKKMLLKEFKSLKNITA
                     ATTEELQEIGLPKNVAQNVFDKLHQE"
     gene            19107..20006
                     /locus_tag="ACG842_000748"
     CDS             19107..20006
                     /locus_tag="ACG842_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0247310.1"
                     /translation="MLEVKDLVKTFGNYTAVDHVSFQIPDGKIMGLIGQNGAGKTTTF
                     RLILDFLTQDQGEVLWNGHHLGEKDYDVIGYLPEERGLYPKVTIQDQLLYFAELRGKS
                     RKEIEPKIDFWMEKFQVKGKKTDKVKSLSKGNQQKVQLIATLIHEPKLIILDEPFSGL
                     DPVNAELLKDGILELKKHGSCVIFSSHNMDNVEKICDHLIMLRNGKMVLNGKVHEIRE
                     SFGRTKLFLESGLSQQEVEEIEGVKKVILHEDNTLEITLEDPEAGKEIFTRATQFGYI
                     PMFNQQPPTLEEIFKLKAGEIDA"
     gene            19999..21261
                     /locus_tag="ACG842_000749"
     CDS             19999..21261
                     /locus_tag="ACG842_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0247311.1"
                     /translation="MRKFWIITKDVYLKNVKSISFLIMILVPFIMLGIIYVAGNFAQQ
                     NSDVNKIGVLADNEQISQQMSQVKTDDFEFKAVDSEEKAQKELSEEKIDAYLVVSTEN
                     DKINGMLYSENSLGQSTQLLIQQQLTGLQSMLRASQLGISPEEVASLSQQASLTRQKV
                     NFDSKGKMTVGEDNSTVQYVVSYVATIVLFIIILTYAQIIAQEIASEKGTRIMEVILS
                     STTAQKHFYGKLTGVLLVALTQMALYGIIFGIGYSQFKDMEMVKNILDGISLDNLIGP
                     FLWYSLLFMIFGILIFSVLAALCGSLVNKAEDTAKAILPVTYLSLGGYMLGLILGASD
                     PNNIVIRITSYIPFLSSYIMPIRLANETVGMGGALISLLVLVAVTFLLMILSANMYKS
                     NVLVYSEGGLWSSLKQSISIMRNERKKA"
     gene            21422..23320
                     /locus_tag="ACG842_000750"
     CDS             21422..23320
                     /locus_tag="ACG842_000750"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphatase class III"
                     /protein_id="HFQ0247312.1"
                     /translation="MDRYLDLLSEKFPSTEDVITEIINLEAILQLPKGTELFLSDIHG
                     EFPAFDHILRIGSGNLKEKIKDLFSEQMTETDISQFTIFTAYPEYALTTDWYKEQDKS
                     QLIHHLIDLLRFTTVKYTRSKVRKGLPKEYSYIIEELLYIDDRINGKKDYVKKIIEQL
                     VLMKEEEKFLTKLAQTIQKSVIDHLHIVGDIFDRGTQAAKVMDRIMDFSSVDIQWGNH
                     DILWIGAYCGSEACLLTLLRIAARYNYLFELEKEYGLNLRPLFSFAHQTYQKNPVFAP
                     KNSKNLSEREQEELEKIHQALSILQFKSEHQLLDRRPEFKMDDRKLLEKIDYEASTID
                     LEGQLYELKGTCFQTIQPDDPKKFLEEEQKVIDSLMYSFQHSLRMQKHMTFLIEKGGM
                     YLTNNQHVLFHGCIPVDEKGQLLAFELDQSYRGKELLGFFEKQITESAKDLTAKEDLA
                     TDLIWYAWSGPFSPLFGKSKMATFERYFLTEPHTHVEKSNPYYHLRDEEWFSEKILAE
                     FDTKEEGSAIINGHTPVKVKKGESPIKANGKVFVIDGGLSKAYQKTTGIAGYSLLCNS
                     YGFQIVTHYPFLPIEQQFAKKSDQTNVKKVIEQQLPRKLNRDTTKGMELQQQITQLKR
                     LLDYRKDD"
     gene            complement(23650..24369)
                     /locus_tag="ACG842_000751"
     CDS             complement(23650..24369)
                     /locus_tag="ACG842_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HFQ0247313.1"
                     /translation="MKPIIGIAGNQLIRATDTFQGNQVSYTPQGFVDAVLDAQGLPLI
                     LPVMSPDSAPQLIGQIDKLILAGGQDVSPQLYMEDPHPKLTETNIQRDQFEQALILEA
                     LKQRKPIFAVCRGLQLLNVVLEGTLYQDLSLYPKWSVKHEQQPTAPQFTTHEVKIVSD
                     SLLSNLLPDPYFVNSYHHQAVKDLSPLLKAIAFSNDGLVEAVQSKDDMHKILGVQWHP
                     ELTHRVHPSEQRLFDFFVNEF"
     misc_feature    24559..24606
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF03071"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="sodium ion sensor (DUF1646 type); this
                     cis-regulatory element may regulate processes involved in
                     with the transportation of sodium ions; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF03071"
     gene            24706..25038
                     /locus_tag="ACG842_000752"
     CDS             24706..25038
                     /locus_tag="ACG842_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0247314.1"
                     /translation="MKQVLEALEQIRKAEEENEKRAQTLRQQLKIYATEKEQELETIQ
                     TNYRQQLKQLVEEKEQFEIQELEKEKMKLKAEADKVQATLKDQYQKHQSQAIGAIIER
                     VKETYGRH"
     gene            25025..27028
                     /locus_tag="ACG842_000753"
     CDS             25025..27028
                     /locus_tag="ACG842_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HFQ0247315.1"
                     /translation="MAVTKMEKVTLISDKRNQERILQAVQGIQNVEIRDLYQNRTNNQ
                     WVEKYFPDTTIIDKEAKLSALGNRLKDIQEAIQFIQHHGDSRQKKLHLKRQELSLQDL
                     EANYSEENFINKLKEVLDLKEKWEQLTEERDRLEEEEEWLLHWQQLDIAPSMYASKTT
                     IFEMGTVSAANFESFKEELSKLDDVYMEEIDYTPKQVYLSYVALKKSVAVIEEIAGKY
                     GFNKETYPYEQNPKEKLQETKQQLSIVHEQQKKITTALGQCSGYIQEFEWVEEVTLAV
                     AEREKIKERFIHATYLIVLQGWVDAEEKQNLLHVLNETVSEEDIYVSFETPNSEEIQQ
                     EVPTKLKNHPLVEPFELLTEMYSLPKYEEVDPTPWMTPFYLVFFGMMVADVGYGLLML
                     IGTILAQKLLVLPRGMKRFVKFFEILSIPSIIWGLIYSSFFGQSLPKELFGIRLPFPI
                     LSTTDDVNTILILSVIFGLIQILVGLFVAAKENIRRKDYLSAVSDGFAWQGILIGIVI
                     ALVGAMLIKNTAFVYLGAGLSIIAALCILIVPIIQTPSKVKGAAKGAYNLYGLTGYIG
                     DLVSYTRLMALGISGGSIGAAFNMLVAFMPPAARFSVGILLIIALHALNMFLTLLSAY
                     VHGARLQYVEFFGKFYTGGGRAFDPLKTAEKYVNINHKKKNNK"
     gene            27047..27517
                     /locus_tag="ACG842_000754"
     CDS             27047..27517
                     /locus_tag="ACG842_000754"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HFQ0247316.1"
                     /translation="MMDYLISQNGGMVFAVLAMATATIFSGIGSAKGVGMTGEAAAAL
                     TTSQPEKFGQALILQLLPGTQGLYGFVIAFLIFINLGNDMSVVQGLNFLGASLPIAFT
                     GLFSGIAQGKVAAAGIQILAKKPEHATKGIIFAAMVETYAILGFVISFLLVLNA"
     gene            27538..28122
                     /locus_tag="ACG842_000755"
     CDS             27538..28122
                     /locus_tag="ACG842_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase V"
                     /protein_id="HFQ0247317.1"
                     /translation="MNAIEKIISQMNEAAEQERAALEQEERMKIDQNFEQKRTQVETE
                     HQKQKEKQIELLEKKYRQLRNRQQVEVRQENLNAKQEFLRRLFADAVTEMENWDESEQ
                     IQFIKNALYSLPLTGKVAFIAGEKSAAYLSQTLLDEWNNELPFMMVLSDETVADQAGF
                     LINDQGVQYNFLFSSLVQDIQGTMSFEIANQLFE"
     gene            28137..29123
                     /locus_tag="ACG842_000756"
     CDS             28137..29123
                     /locus_tag="ACG842_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046961 - proton-transporting ATPase
                     activity, rotational mechanism [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HFQ0247318.1"
                     /translation="MEYHELNPLIRGRELELLSNETFERMIQTNSVEALGEILKSTIY
                     SPYIYDGYEYDFEQNLLKEQSRLFRWLKESAPEPEIVWIYTMRYTFHNLKVLTKAEMT
                     NQNLDHLYIDDGFYSLETLKDAIHTQVSNELPESIMDSIREVRDYFEESTILQGIDVI
                     YDRQFLTEQRRLADKLGYQELLTEVIAFIDLTNITTMARGILQKRTYGFMTTVLSSSG
                     SIEKEVFLNFVDKDMEDYTNFLLETDYADFLRPVIREGNIDLVKLEQLKDDYLSSLYQ
                     EAQTQAFGPLPLLAFLNAKEVESKNLRLLVIGKRSHFESETIRKRVRQVYDA"
     gene            29113..29424
                     /locus_tag="ACG842_000757"
     CDS             29113..29424
                     /locus_tag="ACG842_000757"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HFQ0247319.1"
                     /translation="MTHKIGVVGDKDSVLPFKLFGFDVRYGTTKQTVRQAIEEMAKNE
                     YGVIYVTEQCAALSPETIERYKEQMIPAIVLIPNHQGSLGIGLEEIQKNVEKAVGQNI
                     L"
     gene            29446..31227
                     /locus_tag="ACG842_000758"
     CDS             29446..31227
                     /locus_tag="ACG842_000758"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HFQ0247320.1"
                     /translation="MQIGKIIKVSGPLVMAENMSDASIQDMCLVGDLGVIGEIIEMRG
                     DVASIQVYEETSGIGPGEPVRSTGEALSVELGPGIISQMFDGIQRPLDTFMEITQSNF
                     LGRGVQLPALDHEKKWWFEPTVEAGETVSAGDVIGIVEETKVIKHKIMVPNGIKGTIK
                     TIEKGSFTIDETVCVIETENGDKNLTMLQKWPVRRSRPIKEKLNPDAPMITGQRVIDT
                     FFPVTKGGAAAVPGPFGAGKTVVQHQIAKWADVDMVVYVGCGERGNEMTDVLNEFPEL
                     IDPNTGESLMERTVLIANTSNMPVAAREASIYTGITIAEYFRDMGYDVAIMADSTSRW
                     AEALREMSGRLEEMPGDEGYPAYLGSRLAEYYERSGRVIALGSEGREGSITAISAVSP
                     SGGDISEPVTQNTLRVVKVFWGLDSSLAQKRHFPSIDWIQSYSLYSTEVGKYMDHILQ
                     ADWASMVTEGMRILQEEQQLNEIVRLVGIDSLSDNDRLTLEVAKSIREDYLQQNAFDD
                     VDTFTSREKQFKMLKMILTFGEEGRRALSLGAYLSEIMDGTVSIRERIGRSKYIPETE
                     LEKLDQIIGEIKQTMQTIVSEGGMSND"
     gene            31220..32596
                     /locus_tag="ACG842_000759"
     CDS             31220..32596
                     /locus_tag="ACG842_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HFQ0247321.1"
                     /translation="MIKEYRTIGEVVGPLMAVEKVAGVKYEELIEVRMQNGEIRRGQV
                     LEVQEDKAMVQIFEGTSGINLRDSSVRFLGHPLELGVSEDMIGRVFDGLGRPKDNGPE
                     ILPEKHLDINGEVINPVARDYPDEFIQTGISAIDHLNTLVRGQKLPVFSGSGLPHKEL
                     AAQIARQATVLDSSDDFAVVFAAIGITFEEAEFFMEDFRQTGAIDRSVMFMNLANDPA
                     IERIATPRMALTAAEYLAYEKGMHVLVIMTDMTNYAEALREISAARREVPGRRGYPGY
                     LYTNLATLFERAGRIRGLKGSVTQIPILTMPEDDKTHPIPDLTGYITEGQIILTRELY
                     KSGIQPPIDVLPSLSRLKDKGTGPGKTREDHAATMNQLFAAYAQGKQAKELAVVLGES
                     ALSDVDKIYAKFAERFEKEYVNQGFYTNRSITETLDLGWELLSMLPRTELKRIKDDML
                     DKYLPEGK"
     gene            32603..33238
                     /locus_tag="ACG842_000760"
     CDS             32603..33238
                     /locus_tag="ACG842_000760"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HFQ0247322.1"
                     /translation="MARLNVNPTRMELTRLKKQLTTATRGHKLLKDKQDELMRQFILL
                     IRKNNQLRQEMEESMTEAMSDFVLANASITEPFIEELFVLPAENVDLSIVDKNIMSVK
                     VPVMNFEYDEQLTDTPLEYGYLNSSAELDRSIDRFTTLLPKLLELTEIEKTCQLMASE
                     IEKTRRRVNALEYMTIPQLEETIYYIRMKLEENERAEVTRLIKVKNMGTTD"
     gene            33268..34623
                     /locus_tag="ACG842_000761"
     CDS             33268..34623
                     /locus_tag="ACG842_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HFQ0247323.1"
                     /translation="MIKQSKQIKKRLPAVQLLALGFFLLILVGGGLLTLPFFSNSGHG
                     TNFIDALFTATSAVCVTGLTTLNTAEHWNSAGQFLIMLLIEIGGLGFMMVPILCFALI
                     KKKVSLSTRIILKEALNLEEVSGVMKLMLYILKLAFVIQLIGTVALSFVFVPEFGWLK
                     GIWYSLFHAISSFCNAGFDLLGDSLANHQQNVYLIMVVSALIISGGLGFIVWRDLLEY
                     HQKKKMTIHSKIALTVTGTLLLGGFVVFFFTERNGATLVEGNMFQRLANTFFMSVTPR
                     TAGYYSVDYLHMSHAGLILTMFLMYIGGTSGSTAGGLKTTTLGVLLIQMRSMLKGRTR
                     AEAFGRTIRQGAVLRALTLFFVTLTLCVVAIMILSVTETIPKTSGIEYIAFEVFSAFG
                     TVGLTMGLTPDLTAIGKLIIIALMYIGRVGVLTVVFSLMTKAQRTQSKYKYPEESVMI
                     G"
     gene            complement(34815..36209)
                     /locus_tag="ACG842_000762"
     CDS             complement(34815..36209)
                     /locus_tag="ACG842_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HFQ0247324.1"
                     /translation="MPINSYDNYPLTWRPDKQKLKRPYYRSLIQQLEQDILKNKLQKN
                     TQLPSQRELADYLDLNFTTVGKAYKYGIEKGLLYTNIGSGTFISPNVFESITISTNEV
                     TEHIIDFGLVSSFEECNKFVVPFIQSTSNNPNLVGLLNYQDPKGTNFQLTTAANWLKT
                     QGVIPSLESLSIVSGVQNGLAITLSALFSPGDRIATDRYTYANFIELAQLYHLEIVPI
                     DFDEQGMRPDILIQECRKKKIHGVFLMPSCNNPIGFQISHKRREQLAEVIEKEGLWVI
                     EDDIHSFLTTYYQHTIIPPFQNLLPNQTIYLAGMTKFICSGLRVAYLVVPVHVQSKIE
                     QAIFNINVKTSGLDVEVITQILHSNEANRILETKLSLTKRANQLFDSLFKVEKPTNRV
                     PYYRILPVDPEIDPKIIENDFLANGIRLYHSRRFTTRAQPDAFVRISLSSNNLEILAS
                     GLSIVKEKIKKYEL"
     gene            36691..38079
                     /gene="ltrA"
                     /locus_tag="ACG842_000763"
     CDS             36691..38079
                     /gene="ltrA"
                     /locus_tag="ACG842_000763"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HFQ0247325.1"
                     /translation="MRRPQKTRKLGYHQRASVEHEGYDEVRSTSCGEQRKQDGVENLF
                     GRILDRNNLNQAYLQVVRNKGAAGADGMTVDQLLPYLKEHREELLSNLRQGNYKPQPV
                     LRVEIPKPNGGVRKLGIPTVIDRMLQQAINQVLQPLFEPEFSDNSYGFRPNRSAHDAV
                     KKAKSYYEQGYRYVVDIDMKAYFDTVNHDKLMYFIERKVKDKQVLKLIRQYLMSGVME
                     NGLITTTEEGTPQGGNLSPLLSNIYLNEFDQVLENLGHKFVRYADDCNIYVRSKRAGQ
                     RVMKKSIQFLEEQLKLTVNREKSAVGSPLKRKFLGFCILPTKKGIKIRPHAKAKQRVK
                     SKLKQLTKRNRGRSIQLILKEIKQLMTGWINYYGIGEMKEFMRELNGWLKRRIRQYIW
                     KQWKNPRTKRKNLIRLGIDKAKAYEWSNTRKGIWSVSKSHILHRSLTDKELARQGYED
                     ISLKYQFVHSTY"
BASE COUNT        12628 a         6568 c         8175 g        10763 t
ORIGIN      
        1 taataaaaaa ggaagaatat tcatgtaaac gattttattg tttttttctc ttttatacaa
       61 aaagggtcat aatttcaagt aagcggatga attcatgagg attttttgca tactgtctct
      121 actatactta ggtcactaag aaaaaggagt gatttattat gaaaaagaac atgattggga
      181 cattattact ttcaacactt cttctaggcg gcttggcaac accagctttt gctgagggtc
      241 aagcgacttc aaaaggagat attaccttta ctgaaccaac aaataccgtg gagcctctta
      301 accctactga tccttcaaaa ccagtagagc cagcagatcc ggaaaatcca gcaactggcc
      361 agacaggtcc tttaacactg gatgtagttc ccgaattgcc atttggtaca cacgagattg
      421 aaagcggaac aaaaacgtat caagtagacg cttcaaaaaa cgatacacct tatctccaag
      481 tatcagatcg tcgaggtgta ggtgcagatg gccaagcaca agggtggaac gtgacagttt
      541 cggtttcaga ttttgtaaac ggttcacaag ttttgcaagg cgctgaatta gattttggaa
      601 catccactgt taaaagtacg tcagataacg aatccactgg accaactagt caaactgtaa
      661 ctgggttgag taaagcaagt gcagcgacac caatatttac agcagcaaaa gatcaaggtc
      721 tgggaacatg gctttctgtt tatgatccag caaatatcac actgaaagtg ccgaaagcag
      781 cagctggaac attcacggct gatttgactt ggaacctcgt ggctggccca gtagcataga
      841 gtatgaaata aattcaacaa actgcggtga aaatactata ggtgaaggat tttttaccgc
      901 agttgttgtt gaaaggatgt ggtggacaat gaatcaaagt aaacgactgt ttgtttcgtt
      961 tttttctatt ttaagtctat ttttcatttt tccgtcaatc agccaagcaa aggattcagc
     1021 aggagacttt ggaatcaaac cggtatttcc agagaaccaa atagataaag caatcggtta
     1081 ttttgattta ttagtggcac cggaacaaaa tcagacacta gaagtaatta tcagcaattc
     1141 ttcagatgaa gaacgaacat ttgaggtctc tgtcaatccg gcggtaacga gtgatggtgg
     1201 aacgatcgat tatagtcaaa aaaacccaac gttagatgag acattgccgt ttgatgtccg
     1261 tgacgttttg ctcattgcga aaaaggaaat caatgtatca gcacatgcag aaacaactgt
     1321 acctatagaa gtgaaaatcc cagcaaaaag ctttaaggga cgtgtattag cgggtattca
     1381 tgtttcacct aaagaagaag ctgaaacaga aaatgctaca gaaggagcac aaatcaaaaa
     1441 tcgtattgct tataatctcg cagtcgtttt acaagaaagt caagagacga tagaaccgga
     1501 tctgaaactt ttatcaggag atttggacga ggtcaacgca aagccaactg ttcagcttcg
     1561 tttccaaaat cctcagccta ggattatcag caatttgata tttacgagca agatctttta
     1621 tgaaaatcag ctttatattg aaaatacgtc taatgcattt ttagttgcac caaattcgaa
     1681 ttttcacttg aatcttgatt tagctggtga taaagcaaaa gccggcgatt accgcgcaga
     1741 aatcatagca aaatcaggcg acagtaatga atggcgattt acacaaaact ttacgatcaa
     1801 aaaagaaaaa gcacaaaaag taaatgaaaa ttccgttttt gcagtacaag aacaatcttt
     1861 cccatggctt tatgcaggat tggttgctgc agttctagtg ttatggttga ttttgctgtt
     1921 tttgtgggtc cacaagaaaa agaaaaaagg agaaacaaca tgatgaggtg atttcgttga
     1981 aaacatcgat cttattggga gggattattg caagtttgct gttcttcagc tggcaagagc
     2041 cagtccaagc agataccaaa agttcaggta cgattacatt ttatgatcta agtccaattc
     2101 cgcatccaag tggttcagtc aatggcgcat caaaacagca gacacagcta ccgatttata
     2161 aagaatctaa tcaacatggc gtattcccgg agacgaatga acaaaagatc cgttttcttt
     2221 atttgatcat tggtatagaa ttatggcttc ttgcgtggtt gttgttatgg agaagaaagg
     2281 aaaaggagga tgaacatgtt gctacaaaat aaattttcat tttttcgctc cttctttttt
     2341 acttcgcttt ttgttttaag tatatttatc ttttcacctt cggcagaagc tgttggagca
     2401 acgcctcctg ccatttctta tgatccatac acgtatgaaa atatcgtacc tccaaatggt
     2461 ggacgtagtg cagtcaatgg cgggaagctt gtccaactaa ctggggctaa ccaattgcct
     2521 aaattgactg ctacttattt gaacgatggt tcaaatgatc cttggaaagg tggcacggtc
     2581 cgttttcatt gggaaagcca tgtgaaacta acaaatgtac ttgatttggt gaatatcact
     2641 gcacaaggat ttgcatttgt ggcgatcatc aatttgccgg aacatgtatc aagcagtgat
     2701 gtattaaaag caattgactg gaacaacgcc tacttgtata ttgccggtgc ccccatcaag
     2761 gttactagcg gaagtttatt atcagcagga agccatacct tgcgttttgg gatggggtcg
     2821 tgggatacaa atactctatt gacagatatt attgctatta ttacgcgagg cgaattcaac
     2881 gtggacgata tcccgcttca atttgatgtt acagctaatg tcgcgaagat gacagaaaac
     2941 ggagataaat atgatacgag tccaaacaaa attttgacca agggtaaatt acaaccttct
     3001 atgtctaaaa aggccgtttt ttctgttgat ttttatgatt cgaataatat tatccaagat
     3061 cgtatgttca attcgatttt agctccagct gcaggtagat tttatcctaa attaactggc
     3121 ggaaacttga atccaggagc atatgcattt tctgcaagtg cagagataaa tacgtggaat
     3181 agttacatct caccaagtga ttcaacagga aaatataata catttgctga taccacagaa
     3241 tacgtggatg gaaataacca gaaccttcca ggctctacca atagtcggac attgacgatg
     3301 gggctgactg aaggacgttt tgatcagttg ccatttagtc gctttgatcg cgtagtcaac
     3361 tattttacag gaaaaaacgt tacacaagga gcaatactga ctcatactcc agcaagcaca
     3421 agtggattga atcagacagc caatatcgtg tacagtggga aagatgcgca aggcggattg
     3481 ctttctcctg ttgctttaaa ggtattggag cagtacaagc tagacggaac ggttttacca
     3541 acaaacttaa cagccgatag tcagtgggga aagctccttt cttctgcatc gtaccaagtc
     3601 acggccaatt ggtcagcaac tgaactaaaa aagggttcga tccattacag gctttattcg
     3661 aaaggaaccc aaaaattagc gacaggatgg acagatcaac ttttccaaac gatccaatca
     3721 aataacgggg gtgtaaatac agctacttct acactacctg cattggcttc agggcaatat
     3781 tattttgatt atcgcttggt ggacgatgta cttactaaca tgtatccgtc ttttgcgtac
     3841 aaatggcagt ctgaacaagc tggaatcacc actcttcctc aaataacagt ggctaatttt
     3901 ccatctgtga gtgcagatag tcagctaaaa aaccttagca gacaacctga gacaggagat
     3961 cccctgtctg ctctatcagg agatacgatt caagaaaatg tgacattcac gttaacaaaa
     4021 tctggggatg cgataaccga caaaaaaatc acgatctcac ttcctgaaaa tactacttat
     4081 atcaatggtt ccttaaaatt aaatggtaca actatcgcag ataccggcat tcaacaagga
     4141 gtttctgtcc cagcagatct attgagcaaa ataggagata ccattcatct tacttacaac
     4201 tatcaattaa atactgtaga tacttcttta caatccgtca gcatactgac aaaggcagct
     4261 gtactttcca gcaatattac attagcagat ggtgctaagc ttcctaaccc agttgtccaa
     4321 acatctgcta aaacaattct tgtccccaaa caagaattaa cattagttaa tgtaccagat
     4381 gattttacat ttggtaacga tctccccaaa ccgttgaaga caagttatta tgaatcaaaa
     4441 ggcgattttt cttttgatgt gcgggatact cggcttccaa gcacgtctcc ttggcaactg
     4501 acaggtacat tgacttcttt atttaagaat gatcaaggtc aagaattaag cggaacaaaa
     4561 ttatacttca atcattctgg atcaaagcaa ttgattcagc aaggtcaaaa cacgttgatt
     4621 tatgagagtg atggaacagc taaaggagag gttctcgttg actttcccga taccgatggt
     4681 ttactcttag aggtaaatag cagtacgaat gctcaacctg gcgcaacgta tcaaggcatg
     4741 gtcacatggg aactgacagc tgggccaacc agctaagtga ttagaatttg tatggatctg
     4801 tgataactgt cacagatcct ctttttcaag gatgagaagg aaacttctgc tgttaaaatc
     4861 agaaatgctt gaaggagtgg acttggaact tttcagtttg cttgaaaaaa atgaacaacg
     4921 gcaaatcaaa cttttccaac ttttattaga ggaaaaagga gaggtttccg ttaataacgt
     4981 acttacccgc ttatcagttg atcgggctac cttgaaagaa gatctgtttt atttacaagc
     5041 taacttggaa aatatcaaac aattggaaat tgttatccaa gaagggaata ttttattaat
     5101 cagacatgga aatatagcag cagtcgatgt ttattatcat tgttacttgc gctattctac
     5161 aaaatatcag atacttcttt atctgttgga aaatggctca tttgatcggc agaaacttgt
     5221 catggaactt aatatcagtt cggctacttt gtctcgcaga gtgaaggaat taaatgattc
     5281 tttaaatgag tttcatctga aaataaaaag cgggcggctg accggtccag aaagtcaaat
     5341 tagttatttt tatttccaat tattttggtt tggacgtccc tatcttgaaa acaaaaaaga
     5401 atacagtgat ttatcaggaa acactttttt agatctgctg caaaaagagt ttagttttgc
     5461 tattacagaa gaaggacgat tgaaatttga aatatggctt tccattatga agaagcgaat
     5521 gcgctgtacg aaaaaatcca aaccagatta tagcaatctt cctgttggat tgtttcaaac
     5581 gacacaattt ttattggacc tccatacatt tttgacccgt tacttctttc aatatgcctt
     5641 tccttggaat caaaatgaaa cgttggtttt ttatcttttc atgatcagta actttacatt
     5701 agatacacat aactcgcatg tccaggagtt tttgataagc gaaaaataca aagacgacgt
     5761 agtagctcaa ttgaatcgaa cgtttaaaca atcgatagaa gaatatttct ctttttatgt
     5821 ttttccagaa gagatttcgc aaaagattgc gttgacagtg atgcaaaatc acgctcattt
     5881 tgcctatttt agaggatgga tgaatatttt tggccaagaa ggattaaaac agcggttaac
     5941 tgaagtagaa gataaacaat tcctggtttt atgtgaaaga ttggtcagac aaacatttgc
     6001 tattgttgat tttactgaag tagacgagga aaatgcccgc tttgaattat ttgctcgcta
     6061 tgcatctatt ttacggacgc tgatcaagca gttagaatta cgtctgcaaa ttgcttatga
     6121 tcttccttat gaaaggctaa tggcggatat cgtgaaagaa atcatccagg aacaattgct
     6181 gaatacgatc aaatatgatt tagtcgaata cgagaaagaa aagcagtatg atatcatttt
     6241 gactagccaa ttgaaagagt acccgtcaca gaagtccgct aaagtttttg tattcactag
     6301 caacgaatat aattatgatt tcccctattt gaaccaattt ttaaaagaat gttatttaga
     6361 aaaattgaat ttaaggatga ataaaacatg aaaatcgtga tcattggtgc ttcccatgaa
     6421 ggcatcacag ctgccctaaa tctaagaaaa cttcaaccga aagcagaagt tttactgatc
     6481 gatgaagatc ataaagatgg actaggctat gtttcaaatg gaatcaacct ttatttaaaa
     6541 ggaaagatac gtagtttagc tgaagttgcc cataacatgc ggactcttca agaaagtggg
     6601 gcaaaactga tcaccgaatg gagagtaact gaacttgatc cagacaaaca tcagctcatc
     6661 ctcgcatcca aagaaggaaa aaacgagacc ataacctatg ataaattaat cgtagcgaca
     6721 ggttcctctc ctgtcacctt gtataaacaa attgaagctg aaaatgtcta cacctataaa
     6781 aatctagtgc aatctaagca agtacttgcc gctttaaaag aagcaaaaga agtagtgatc
     6841 ttcggtgctg gttacattgg attggaatta gcagatgctt tgagaaataa aggatacatt
     6901 attcatctag ttgattatat gccgaatgtt ttatcaagat atttcgataa ggatatgatc
     6961 aacagttttc aaaatcagct gcaaactaag caaataaatt tttatccaaa tgaattcttg
     7021 atcgattgga aaaaatcaga agagaaagta gtatccgtac aacttctgtc tcaaacaata
     7081 aaggcagata tggtcatttt ttctgcccaa actcgtccta atacgacatt gttaaaagaa
     7141 aaagtggctt tgtatgagga tgagactgtg atagtcaatg aatatctgca gacaagtgat
     7201 ccggacatct atgcgattgg agatatcgtc ccagtcagtt ttgacaaaaa taaacggcat
     7261 ctctttttac ctttagttac tcgagctgta catatggcaa gagcagtagc gctaactttg
     7321 agtggtcagc caaccgctta cgacctcaga caaaagataa cagcagcagt cattatagac
     7381 tactttttag gaactgtagg gctgacagaa gatgaagcgc cattcttgga acaaaatacc
     7441 ggttcgtgtt caggagaatt tgatctattt ccacaatacg acgaagaaaa caaaacagta
     7501 aatgcaaagc tgatctacca tccggataca ttagaaataa ttggcggaca gctgataagc
     7561 caagaattct tattatcaga tttgaacctg cttgcggata tcgtgaaaca caaaactacg
     7621 attccacagc tagcagttga ggattttgga tttctgtctg aatacacctc tcgttttcac
     7681 tacctcaatg agttagcttt taaagtacta gctaagagag caaatagcaa tagagtagat
     7741 aaaagactgt gagaaatatc acggtctttt attgtgtggg gatataataa atattaatca
     7801 atcgagaata aaaaattcat ataatgaaat agcggaagaa ttttttgagc ataatattcg
     7861 ccaaaaattt atcaagtgat aaaattgttg tataaaatga ttatttcagg aggaggagtt
     7921 aaatgttttt agcattaaat gaaattatgc attcaaagtt acgttatgcg ttagttgcag
     7981 gggtgatgtt tttgatcgcc tacctggtat tttttttgac tgggttagct tatggcttag
     8041 ctcaagataa tcgaacagca gtcgacaagt gggaggcaga ttcgattgtg ctttcgaaag
     8101 atgcaaattc aaatctaggg atgtccatga ttactaagaa gatagcagaa gaagtcgaag
     8161 gtgggaaagt ggcgtatctt gcccaaacac ctggagtcgt gactagtaaa gacagtacag
     8221 aagaaggaaa aatcaatgtt agcttcttcg gtatcgataa aaatcaattc atcatgccca
     8281 atcttgttga aggaaaagct tttgataacg atgatgaggc agtaggcgat attagtttga
     8341 aagaagaata tggtctagcc ataggagata cagttaagtt atctggtagt gacaagacgt
     8401 ttaaactaac tggctttacg gatcatgcca aattcaatgt ctcaccagtt ctctatacaa
     8461 ccatcaatgc ttaccaagaa atccgcttcg aaaaagaaga tacaagtgaa aatgcacgga
     8521 ttaacgcaat agttgtcaga ggtaaaatca gtaatttacc tgaagattta gaacagatca
     8581 aaatttctaa attcatcaat gaattacctg gatataatgc gcaagtattg acttttggat
     8641 ttatgatcgg atttttgatc gttattgcgg ctattgtgat cgggattttc atttatgtac
     8701 tgacgatgca aaagattaat atctttggtg taatgaaagc acaagggatc acaggaggat
     8761 ttattgcgcg ttctgtagtg gcacaaacat tcattttatc atttgttggt attcttcttg
     8821 gtctattagg aacagtggga acatcccttg ttctacctga tgcggttcca ttccaaagca
     8881 attggctgtt ttttggcgtc attagcttgt tgatgttagt agtagctgtg ttaggcgcac
     8941 tattttctgt tcggacaatc gtaaaaatcg atccgttgaa ggcaattgga taagaaggag
     9001 gaagaacatg aaagcaatag aatttaaaga agtagaaaaa agtttccaag atggcgacca
     9061 agtagttaaa gcattgaaag aaacaaccat gagtatcgat aaaggggaat ttgttgcaat
     9121 catcggtcct tctggttcag gtaaaagtac ttttttgact ttggcaggcg gattgcagac
     9181 gcctagcagt ggctctgtat ttatcaatga tcaagaattc agtaatcaaa aagaaagcga
     9241 acgcgttaaa ctacgtttta aagaaattgg ctttattttg caagcttcca atctggttcc
     9301 ttttctgaca gtaaaaaagc agttattatt agtagataag attaaacatg aacatcgtca
     9361 aaaagaagca aacgaattat ttgagcagct tggcgtggat aagttgatca acaagtatcc
     9421 agaagaacta tctggtggag aacggcaaag ggtcgcaatc gctcgtgcat tgtacaatga
     9481 tccgtccatt attctagcag atgaaccaac tgctagtttg gattcagaaa aggcacgaga
     9541 agtagtcgat atcttggcaa aagaatcaaa agacaaaaat aaagcaacga tcatggttac
     9601 tcacgataca cggctgattg acaaatgcga tagaatattt atgatggaag atggtgtatt
     9661 gaaagaaaaa gaataaccaa aaagcaggtg gaaaaatggt ccatcgatac aacaaagata
     9721 cacagcaaaa aattttgaaa gaagcgactg acttgtttat gtccaaggga tatctcggca
     9781 cttcaacaag agaaatcgca caaaaagttg ggatcaccca gcccaatctc tatcattatt
     9841 tcggtgataa agaaaaacta tatacggcag ttttagaaaa ccacttaaaa gatgttggaa
     9901 aagctttgag agaaatcgtt caaacaagcg aaacgggatt tcaagtaacc ttgacaaaaa
     9961 tggcgcagtt tttgattgag acacatctgg tcgatttgtt tctgatgctt cacgatcttg
    10021 aaagcaatct ttcaaaggaa acaagagatc atcttttctt tctatggaag aaaaattatc
    10081 gtgaaccttt tgaagaaatc ttttcagcaa accaatctgt gctaagaaat gggatttcaa
    10141 aagaaatcgc agcaaggcat tttttcttag tgttagcacc ttacatcact cggtcgaaaa
    10201 gacctgtgga agaaccgcta aatgtggcgc aattgatcga tttatatttg aatggtgtat
    10261 taggagaagc gtaaagctgt tatataggga gaaaatagtt aggtgaagaa gcaagatatg
    10321 acgaagatcg ttatatcttg cttctttata taggtagttt ttcataaata caaatttgtt
    10381 attctctcat gattatcagg gtatattttt atttattatg tcggtgttcc aggaagtgtt
    10441 ttaatttaat aacgaagaaa ttaattgttt gtagaatgaa ggaaactatt tattgggtag
    10501 atgaatcacc acgaatagtc aaaaggagat ttcaggatat gccaaaaatg aaaaaaatca
    10561 ttttagcagt aatgttattt tctacggtta tatcttttac agcccctttt ttgcttactt
    10621 aagctgattt taagttttgg cccttagtct ggatctacga ggtgagagtc aagaatgaaa
    10681 aaaataattg gagcttttgt atttgcaatc attatcatta ttatctgttt tcaaatagat
    10741 gtttttggtc aaaaaaagat agttggacaa acggtcagca gtacgaacag tacggttaca
    10801 aagacaaagg aatcatttga tgatacagaa caagagacat taatcaaaat cggaaaaaat
    10861 gaccgtacac tatatacaga tgactcgcta actaccccac ttgcaactat aaacggcgga
    10921 gagctggcgg agtttctttc tgaaacaaaa aatgcttatc agatcaagac aaatgatggc
    10981 tatactggat atttagccct cgaagatgga accaaagtca caaagaatat acaaacaaaa
    11041 ccggaagagc tatctgatgc agtgattgta ttagatcctg ggcatggcgg aaatgatact
    11101 ggggcattaa gcaatgatga acaaacagaa gaaaaagata taacattaag tacagccata
    11161 aaagtaaaaa aggcgctaga ggatgctgga gcaacggttt atttgaccca tactacagat
    11221 gaattagtac agcttggaga tatatgcgac tacagtgaag aaaaaaaggc agatgtcttt
    11281 atcagtttac acgcagactc tacggaatat gcgaatgaag caactggaat cacgacatat
    11341 tattactatg gacaagagga aacattagca cagacaattg ctgactcctt cactgatctg
    11401 cctctggatt caagaggaat ctccactggg aactatcaag ttcttcgaga aaatcttcaa
    11461 ccttctattt taatcgagat gggttatatg aataatgata gtgatttaaa ggaattagta
    11521 acagacagtt atcagcagca gattgctgca agtttgacac aagcattgac cacttatttt
    11581 cagtaataaa taagcccagc caaacaaaaa tagctcctca tatttttcgt ttggccgggc
    11641 ttattatcgc agatgcaagc ttttaaacac cgtttaagga agaggtcgtg agcctgccgt
    11701 tcaactctga gtcacaagga gcaatttcaa aaaacagctt ctcatgtttg atgactattg
    11761 tgacttgtga ccgaggagtt ggcatgagaa caccgtttaa gaaaaaggtt gtgaaatcaa
    11821 cgttttgttc tgagtattag agaaacaaac ggaaaaatag ttcctcatat tttgccgttt
    11881 gttgagctaa taccgaggat cagatgattg aacaccgttt attcggaaca agggactgtg
    11941 acatgacttt tgtcacagtt ccattatttt tttagtagct tcaaagcttt ttctgtattg
    12001 gcaaaatgaa gtttggcata tttttcaagc ataggcatgc cgcctttctc caatagtttt
    12061 gccgctactt ggtcagattt cgttccagct cctgacggaa gggtgattcc agcttcagct
    12121 gatgctttgt ctaactcttc caaaaagctg gcacgactga tgatagaagc agcagcaaca
    12181 gctaaatgat attgttctcc tttagtaacg aagtacagtt tttcttgtaa ttgatttttt
    12241 tctaaacgcg catatttttt aaaatttgct tccggcgtaa actgatcgat caaaatcgct
    12301 tctggttttt ctggctcgat tttttttagc agcaaatgga tcgcttgatt gtgcaaagcg
    12361 actttcatcc gaacggcatt gtattttggt tggatctcat tgtatttttc tggagataag
    12421 acaagcagtt tataggggat ggtttttttt aagatgtcag caagctggat gatttggcta
    12481 tcttttaact cttttgaatc tctaacacca agtttacgta attctgcaag ctgatcacgt
    12541 ttcacataag cagcacatac tgttagcgga ccgaaatagc tgccatttcc gacctcatca
    12601 ctacctaaaa cggataattg actgatattt tttggcaaag aagaggctgt ttttgctggc
    12661 ttggcttgtt tatcctgtgc ctccccccat ttatcagctt ctattgctgc attcgtacct
    12721 tgaaacatca cttttccgga tgtataagcg gtaatggtcg tcccgtcttt ttttgcagca
    12781 aagctagtat aaggaatctg tttatttaac aaataagagc gatagtatgc tttcatttga
    12841 ctaagttggt cgtttgttgc tttgataatg acgttgctca taaaattctc ctcgttttta
    12901 tacataaaac tataccgcaa ctcacgactt ttcgttttct aagagattgc gagacatagc
    12961 atttttttaa agtttatcat ataatagtta agtatgtgaa aaatatttgt ggagaacggt
    13021 gaaagaacca acttcatggt acactaaata aaactttcat caaataggag gaaatgattc
    13081 gtatggcgca cgaaaaaaca cgctacaaag cggtgattgc caaccaaaca tatactatta
    13141 tcggacggga aaccaagcat catatggata tcgtaacgaa gctgatcaat gaacagttag
    13201 cagaattgaa acaattatca ccacaaatgg ataatgaaca agcagcaatc ttgatggctg
    13261 tcaatgcgct ttcggatcaa ttgaagaaac aagaaagaat attggagctg gaagaagaaa
    13321 cagcagaatt aaagaaaaaa atgatcaaat ttacagagct tgaaaatcgt gtgaaacgca
    13381 ttgaagcaat cgagaacgaa gctcgggaag tattgaaaga aaatggtcaa gcagatcata
    13441 ccattaaaaa tcatgtggaa gcacaacaga ttttaaacga gaaacgaaaa ggacaaatta
    13501 aacaaaaatc ttctaattaa gatcctttta tcaaaataag aaaaatttaa aaaagttaga
    13561 agagaaagga acgtagcatg ctgagtttgc tgattttatt tttattgcta attgcgtttt
    13621 tttctggagc acgtcgcggg tttgctttgc aaatcatatt cgcgattgga tatgtattgt
    13681 catttattgc tgcccagcat ttttacaaac cattagcaag tcatttggaa ttatacattc
    13741 catacccagc tgtcacccca acatcaaagt tagcattttt tgatcaggta tttgcctttc
    13801 acctagatga agctttttat gcaggaactg cttttctgat tattttgcta attggctggc
    13861 tgcttacccg atttgtgggg gtttttgtcc atggattgac gtatgtgccg atcttgagac
    13921 aggtagactg gattgcagga ggcatcctgt cactaatcat ggcttacgtt actatatttt
    13981 tgattttgca gttacttgcc tttgttccat tagatgtggt ccaaaatcaa ttcggcgagc
    14041 atagtttcgc tcgttttatc gttgagagaa cgccatttct taccaacaag atgtatgagc
    14101 tttgggtaac gaatattttg aattaaggaa aatagtgctg gaaaatattt cgttttttgt
    14161 acagagtagt ttatttaaga aggctgcgta gcgtatgtcc ggcacagctc ttttttcata
    14221 gtgaaagata aggtgataaa atgaataacc gaatccttga aacattggaa tttgaaaaag
    14281 tcaaacagat ggtcagacaa tttgtagtga ctgcacaagg aaaagaagaa ctggcagaat
    14341 tggtaccagt gagtgagaaa caaacgatca caaactggct tcaagaaact gaggatggat
    14401 tgaaagtgca acgtctgcga ggcgggatcc ctatacctaa aattgaaaat atccgtcccc
    14461 atatgaaacg gattgagatc ggagcagact tgaatggagt ggagcttgca caagtttcca
    14521 gagtattgtc tacgacaagc gaattgaatc gtttcattga tgatctttct gacagtgaaa
    14581 tcgaatttgc acgtttgtat atgtgggcag atcagttagt cacgatccct gttttgagcc
    14641 gccgtttaaa ggaagcaatc gatgaagacg gacgcgtgac tgatgacgct tctcctgagt
    14701 taaagagcat aagacagaac attcggcgaa gtgaacaagc tgttcgtgaa cagttagatg
    14761 ggatcgtccg tgggaaaaat gcaaagtatt taagtgatgc gatcatcacc atgcgaaatg
    14821 accgctacgt tatcccagtc aaacaagaat accgcggtgt atttggcggt gtagtccacg
    14881 atcaaagtgc ttcaggtcag actttattta ttgaaccaaa acaagtggtg gatttgaata
    14941 atcgtttgcg tcaataccaa atcgctgaac gaaatgagat tcaacgtatt ttaagtgaac
    15001 tttctgctga acttgttccc catcgtcaag aaatcatcca caatacttat gtgattggta
    15061 aaatggatct gatgaatgca aaagcacgat ttggtaaaga agtaaaagcc atcgtgccgg
    15121 gaatcagtga agacaatcat gtcgtcttga aacaagcaag acatccactg atcgatcagg
    15181 aaaaagtagt ttctaatgac atcacaatcg gaaaagacta tcaagccatc gtcattacag
    15241 gacctaacac aggtgggaaa acgatcacgt tgaagacttt aggtctgtta caactgatgg
    15301 ggcaagcagg attaccgatt cctgcaggtg aagaaagtca aatcggtatt tttgaggaag
    15361 tatttgcgga tataggagac gaacagtcga ttgagcaaag tctatcgacc ttttcttctc
    15421 atatgacgaa cactgtagat attttatcaa aagtcaatga aaaaagtctg gtattatttg
    15481 acgagctcgg tgcaggaaca gatccgcaag aaggtgctgc tctagcaatc gcaatcttag
    15541 atgatcttgg taaaaagtct gcttatgtga tggcaacaac gcattatcct gaattgaaag
    15601 tttatggata taatcgggca aatacgatta atgccagcat ggagtttgat gtggatacat
    15661 taagtccaac gtatcgtttg ctgattggcg ttcctggacg aagcaatgcg tttgaaatct
    15721 ctagccgatt aggtcttgat acagaagtta ttgatgaagc gaagcagttg atgaatgatg
    15781 agagccaaga tttaaacgaa atgatcacag atttagagaa tcgccggaaa atggcagaga
    15841 ccgaatatct ggaaatgcgt cattttgttt ctgaggcaca agaacttcac gatgatttga
    15901 aagaagctta cagctatttc tttgaagaac gtgaaaaaga aatggaaaaa gcaaagaaaa
    15961 aagcaaatga agtcgtgtca gaggcggaag aaaaagcaga aaaaatcatt gctgatattc
    16021 gtaaaatgca acagcagatt gggcaaggaa atgtgaaaga gcatcagttg atcgatgcaa
    16081 agacccaatt agcaaatcta catcaagaag aaactttgaa gaaaaataaa gtattgaaga
    16141 aggcgaaaga acaaaaaacg ttgaaaccgg gagacgaagt gttggttacg acttacggcc
    16201 aaagagggac gcttttacgt aaaaacggga atcagtggca agtagaaatc ggtatattaa
    16261 aaatgaatgt gtccgaagac gagctgacgc ctgttgctcc tcaaaaagag ccgacacaac
    16321 gagttatcca tgctgtacga tctgaatcta gtagtcacgt gccgaatcaa ttggaccttc
    16381 gaggaaaacg atacgaagaa gcgctaagtg aggtagatca atatttggat tctgccattc
    16441 tagctggtta tcctcaagtg actattgtcc acggaaaagg aacaggcgca cttcgcaagg
    16501 gaattactga ttatcttaaa aaccatcgga gcgtcaaaag ctttgagttt gcaccggcaa
    16561 accaaggcgg aaatggtgcg acaattgtta aatttaagta aagcttacta aaggtaagca
    16621 gatggttcgc tatccaagta tagaaatgct atactagtca tagattttaa aaatggaggt
    16681 ctttgatatg tcacaagtaa ttacagataa gacatttaat gaagaaacag ataaaggact
    16741 tgtattgatt gatttttggg caacttggtg tggtccatgt cgtatgcaag caccgatttt
    16801 ggatcagttg gaacaagaat atgatgaaga agaattcaga attgctaaaa tggatgtgga
    16861 tgaaaatcca gaaactcctc aacaattcgg tatcatgagc attccaacat taatgttgaa
    16921 aaaagatggt caagtagtag aaaaagctgt tggtgttcat tcaaaagaac aactacgtca
    16981 aatgatcgat caatatttat aaaaacttac agcaaaaata agctgagggt gggactgaat
    17041 ccagttccgt cctcggtttt tttgcggttg agttaaggtt attcaaatta atattttttg
    17101 agtataatta cgttgtcagg ctggattgtt tttttgacag acatgctata ataactttaa
    17161 ttgaatatga aggggagtac cagatgaacg aacgaatcaa aaacaaatta gctttacttc
    17221 ctgatcagcc cggctgttac ttgatgaaag ataaaaatgg aacgatcatt tatgtcggca
    17281 aagccaaaat attaaaaaat cgtgtccgct catattttac tggtagccat aatacgaaaa
    17341 cggaacggct tgttagtgaa atcgaagact ttgaatacat cgttacagaa tcgaatatcg
    17401 aagcattgct cttagagatc aatctgataa agaaaaatga tcctaaatac aacattatgt
    17461 taaaggatga taaaacctat cctttcctta agataacgaa tgaaaaatat ccccgtttgg
    17521 tgattactcg taaggtacta aaagacaaag cactgtattt tggaccatat cccgatgtcg
    17581 gtgcggcgaa tgagacaaag aagatcctcg atcgattatt tcctctgaga aaatgcaaac
    17641 ctagtcagac gaaggagcct tgtctttact atcatttggg acaatgcctg tgtccttatt
    17701 atttcgacgt agatccgaaa gtctatacga aaatcgttga tgaagtgaaa aactttttaa
    17761 acggaggaca cgaaaaaatc gagtcagaga tccatcagaa aatggaaaaa gctgctgaaa
    17821 acatggagtt tgaaaaagct gcagaatacc gagaccagat ccgtgccatt gagacaatca
    17881 tgacttgcca aaaaatgaca aatactgatt tactggatcg agatgtattt ggttatgcga
    17941 ttgataaagg atggatgtgt gtccaagtgt ttttcgtaag acaaggaaag ctaattgaac
    18001 gagatgtttc aatgtttcct ttctataacg aagcagaaga agatttcttg acttacctcg
    18061 gtcagtttta tcaggaaaat gagcatttca ttcctaaaga agtcttgatt cctgatacga
    18121 tcgacaagcc cagtgtagaa gctttgttag ctacgaaagt gctgcaacca aaacgaggag
    18181 aaaagaaaaa attagttgaa ttggcgagta agaatgcctc agttgctttg aatgaacggt
    18241 ttgatctgat tgccagaaaa caggaacgga cgatcggagc agttgaacgt ttaggaaacg
    18301 cgatgaatat cccgacccca atacgtatcg agtcttttga taactctaat atcatgggga
    18361 cgaatcctgt ggctgcgatg gtcgtattca ttgatggaaa accggcaaga aaagaatatc
    18421 ggaaatataa aataaaaaca gtcaacggac cggatgatta tgcatcaatg agagaagtca
    18481 tctatcgcag atattcgcga gtgattcgcg aagggttgcc tttacctgat ttgatcttga
    18541 tcgatggagg aaaaggacaa gtagatgtgg caaaagatgt cttagaaaat caattaggaa
    18601 tcgatattcc tattgctggc atggtaaaaa acgataaaca taaaacaaat gagttgctat
    18661 tcggtacaga tttggctgta gtaccgcttg agcgaaattc accagagttt ttcttactcc
    18721 agcgaatcca agatgaagtc catcgattcg caatcacttt ccatcggcag ctacgcagta
    18781 aaaacagttt tgcttcccga ttagatgaaa ttgaagggtt aggaccaaaa cgaaaaaaaa
    18841 tgctgcttaa agagttcaaa tcgttgaaaa atatcacagc agccacgaca gaagaattac
    18901 aagaaatcgg actgccgaaa aatgtcgcac aaaacgtatt tgataaatta catcaggaat
    18961 gaggaaaggg cactttacaa agaaagtaaa gtgtcctatc attttatgta caacaaaaag
    19021 agcgtttacg ttttgttggt aaataataaa tcgcttacgt ctggtgtcta aggatatatg
    19081 tgatttcttg aagaaggagt gaagaaatgt tagaagtcaa ggatcttgtt aaaacttttg
    19141 ggaactacac agctgtcgat catgtctctt tccaaatccc cgatggaaaa atcatggggc
    19201 taattggcca aaacggggct gggaagacaa cgacttttcg tttgatcttg gattttttga
    19261 cacaagacca aggagaagtg ttatggaatg gccatcatct tggggagaaa gattatgatg
    19321 tcatcggtta tcttccggag gaacggggac tttatccgaa agtaacgatt caagaccaac
    19381 tgctttattt tgcagaactt agagggaaat caagaaaaga gattgaacca aaaatcgact
    19441 tttggatgga aaaatttcag gtaaaaggga aaaagacgga taaagtaaaa tctttgtcta
    19501 aaggaaatca gcaaaaagtc cagctgatcg ccacgttaat ccatgaacct aaattgatca
    19561 ttttggatga gccattcagc gggttagatc cggtaaatgc tgaattgcta aaagatggaa
    19621 tattggaatt gaaaaaacat ggttcatgtg tcattttttc tagccacaat atggataatg
    19681 tagaaaaaat ctgcgatcat ttgatcatgt tgcgaaacgg aaaaatggtt ttgaatggaa
    19741 aagtacatga gattcgtgaa tcatttggcc gtacgaagtt attcttggaa tctggtttaa
    19801 gccaacagga agtagaagaa atcgaaggcg tgaaaaaagt gatccttcat gaagacaata
    19861 cgttagagat taccttagaa gatcctgaag ctgggaaaga aatctttact cgtgcgactc
    19921 agttcggcta cattcctatg tttaaccagc aaccgccaac tttggaagag attttcaaat
    19981 tgaaagcagg tgaaatcgat gcgtaagttt tggattatta ccaaagatgt ctatttaaaa
    20041 aatgttaagt cgatttcttt cttgatcatg atattagtcc cttttatcat gctgggtatc
    20101 atttatgtag caggaaactt tgctcaacaa aatagtgatg tcaataaaat cggcgtattg
    20161 gctgataatg agcaaatcag tcagcaaatg agccaagtca aaacagatga ttttgagttc
    20221 aaagcagttg attctgaaga aaaagcacaa aaagagcttt cggaagaaaa aatcgatgcg
    20281 tatttagtag tctcaactga gaatgataag ataaatggga tgctttatag cgaaaattca
    20341 ttaggacaaa gtacacaact actgatacaa caacaactga ctggcttgca atcgatgctt
    20401 cgagcgagtc aattaggcat ttcaccagaa gaagtagcta gcttgagcca acaagcaagt
    20461 ttgactcgac aaaaagtcaa ttttgattca aaaggaaaaa tgacagtcgg agaagataac
    20521 tcgacagtcc aatacgtcgt aagttatgta gcaacgattg tcttgttcat tattattttg
    20581 acgtatgcgc agatcattgc acaagagatc gcttctgaaa aaggcacgcg gatcatggaa
    20641 gtgatcctgt ccagtacgac tgcacaaaag catttttacg gaaaattaac aggtgtttta
    20701 cttgttgcat taacgcaaat ggcattatac ggtattattt ttggtatagg atacagtcaa
    20761 ttcaaagata tggaaatggt caaaaatata ttagacggta tctcattgga taacttgatt
    20821 ggaccattct tgtggtattc tcttctattc atgatctttg gcattttgat tttttctgtt
    20881 ttagcagctt tatgtggctc tttggtcaac aaagcagaag atacagcaaa agcaattttg
    20941 ccagtgactt atctatctct aggcggatac atgcttggat tgatactagg agcttcagat
    21001 ccaaacaata tcgttattcg gatcacttcg tatattccat tcttatcttc atatattatg
    21061 ccgattcgct tagcaaatga gacagtcggt atgggtggag cattgatttc cttgttggta
    21121 cttgtagccg taacattctt attgatgatc ttgtctgcaa acatgtacaa atcgaatgta
    21181 ctcgtttata gcgaaggcgg attatggagt tcattgaaac aatcgatttc aattatgagg
    21241 aatgaacgaa aaaaagcata aaacaatcct aaaatacaga aggaagctgg acatctattg
    21301 ttcccagctt ctttttgtat agacctattt tatttatttt actatattgg atatatattc
    21361 attgaaaata gtgtataaca actttataat attgagtata ccaatttttg gaggcgtttt
    21421 aatggaccgt tatttagatt tactttcaga aaaattcccc tcaacggagg atgtcatcac
    21481 ggaaatcatc aatctcgaag caattttgca gcttccaaaa ggaacagagt tatttttgag
    21541 tgatatacat ggcgaatttc ctgcttttga tcatattctg cgtatcggtt caggaaatct
    21601 taaagaaaag atcaaagacc ttttttctga acaaatgacg gaaacggata tcagccaatt
    21661 cactattttt acggcttatc cagaatatgc attaacgact gattggtaca aagaacaaga
    21721 taaatcgcag ctgatccatc atttgatcga tcttttacgg tttacgacag tcaaatatac
    21781 ccggtcaaaa gttcgaaaag gattgccgaa agaatatagc tatatcattg aggaactctt
    21841 gtatatcgat gaccgtatca atggaaaaaa agactatgta aaaaaaatca ttgaacaact
    21901 tgttttgatg aaggaagaag aaaaattttt aacgaaactt gcacagacga tccaaaaatc
    21961 tgtcatcgat catttgcata tcgttggaga tatttttgac cgaggaactc aagcagccaa
    22021 agtgatggat cgaatcatgg atttttcttc tgtagatatc caatggggaa atcatgatat
    22081 tttgtggatt ggtgcgtatt gcggctctga ggcatgttta ctgacacttt tacggattgc
    22141 agcacgctat aattatttat ttgaattaga gaaagaatat gggttgaatt tacgtccatt
    22201 gttttctttt gctcatcaaa cttatcagaa gaatccagta tttgcgccta aaaacagtaa
    22261 aaatctatcc gaaagagagc aagaagaatt agaaaaaatc catcaggcac tttctatcct
    22321 tcaattcaaa tcagaacatc agttattgga tcgtcgacct gaatttaaaa tggacgatcg
    22381 taaattgctg gaaaaaatcg actatgaagc atcaacgatt gatttagaag gtcagctcta
    22441 tgagttgaaa ggaacttgtt ttcaaacgat ccagccagat gatccaaaaa aattcttgga
    22501 agaggaacaa aaagtgattg atagtttgat gtattcattc cagcactctt tgagaatgca
    22561 aaaacatatg acgtttttga tcgaaaaagg gggaatgtat ctcacgaaca atcagcatgt
    22621 gctgtttcat ggctgtatac ctgtagacga gaagggacag ctcttagcat ttgaattaga
    22681 ccaatcttat cgcggaaaag aactgctggg cttttttgaa aaacagataa ctgaaagcgc
    22741 aaaagatctg actgcaaaag aagatttagc aaccgatttg atttggtatg cgtggagtgg
    22801 gccattttcg cctttattcg gcaagagtaa aatggcgact tttgaacgtt attttctcac
    22861 agagcctcat acgcacgtcg agaaaagcaa tccttattat cacttgcgag atgaagagtg
    22921 gttcagtgaa aaaattttag cagaattcga cacaaaagaa gaagggtcag caatcattaa
    22981 tgggcacacg ccagtaaaag taaaaaaagg ggagtcaccg atcaaagcaa atggaaaagt
    23041 atttgtcatt gacggaggtt tatccaaagc ttatcaaaaa acgacaggaa ttgccggtta
    23101 ttcactatta tgcaattctt atggtttcca aatcgtgaca cattatccgt ttttgcctat
    23161 tgaacaacaa tttgcaaaaa aatccgacca aacaaatgtg aaaaaagtaa tcgaacagca
    23221 actaccacgc aaactcaatc gtgatacaac aaaaggtatg gaactgcagc agcagattac
    23281 ccagctcaaa aggctgttgg actatcgaaa ggacgattga gaagaattcc agtgaacatt
    23341 gttccataga ctggagactg agacaaaagt cgtgtcgcag tctctttttc cggataaacg
    23401 gtgttcttct gccaactcct cggtcacaag tcacactatt caacaaacat gaaaagctgt
    23461 tttttgaaat tgctccttgt gactcagagt tgaacggcag gctcacgacc tcttggtata
    23521 aacggtgttc aaaagccagt ttctcggtat taagccaaat ttatgcaaaa atgtgaaaag
    23581 ccattttcac aaaatttttc ttaatactcg aaactaaaca gctttttcac aaccccctca
    23641 gaaagtctat taaaattcat tcacaaagaa atcgaataag cgctgttcag atggatgtac
    23701 acggtgggta agttccggat gccattgaac accgaggatt ttatgcatat catctttgct
    23761 ttgaaccgct tcaacgagtc catcgttaga aaaagcgatg gcttttaata aaggtgaaag
    23821 atctttgact gcctgatgat ggtaagaatt cacaaaatat ggatcgggga gaaggttgct
    23881 taacaaactg tctgaaacaa tcttgacttc gtgagtcgta aattgcgggg cagtgggctg
    23941 ttgttcatgt tttactgacc actttggata taatgaaaga tcttgataaa gtgtaccttc
    24001 caaaacgaca ttcaatagtt ggagtccccg gcagactgca aaaattggtt ttctttgttt
    24061 caatgcttcc aagatcagtg cctgttcaaa ttggtctcgt tgaatatttg tttccgttag
    24121 ctttggatga ggatcttcca tgtaaagttg tggactgaca tcttgaccac ctgcaagaat
    24181 aagtttgtcg atttgtccaa tcagttgagg agctgaatct ggagacatga ctggcagaat
    24241 aagaggcaat ccttgagcat ctaaaactgc atccacgaat ccttgcggtg tatagctcac
    24301 ttgatttcct tggaaagtat ctgttgctcg aatcagttga tttccggcaa tgccgatgat
    24361 tggtttcatt tgtattcccc ctaaagtaga cttattgtaa caaggattta aaaaaaatta
    24421 aactatgtaa gacttgcccc tatacttatc tgttcttgct gtgttactat ctgtaaagag
    24481 aaatttcgtg tgaaattcat gacgatagtt gaaaatattg cttaatttgt tataataaaa
    24541 agtaagcaga aataaaaagg ttgggcgtaa gcttcaagga tctagttctt caaggggtga
    24601 gaaggacatt ttattgaagg ggcctgccta tactaagtta tctttagagg tacatacttt
    24661 caatgaaaaa agaaagcatg tacctctttt ttaaatcgaa aggaagtgaa gcaagtgtta
    24721 gaagctttgg aacaaatccg aaaagcagaa gaagaaaatg aaaagcgtgc tcaaacactg
    24781 cggcagcagc tgaagatcta tgcaacagaa aaagagcagg aattggaaac gatccaaaca
    24841 aactatcgac aacaactaaa acagttggtg gaagaaaaag aacaatttga aatacaagaa
    24901 ttagaaaaag aaaaaatgaa attgaaagca gaagcagaca aggtgcaagc aactttgaaa
    24961 gatcaatatc aaaagcatca gagtcaagcg atcggtgcga tcattgaaag ggtgaaagaa
    25021 acttatggcc gtcactaaga tggaaaaagt gaccctcatt tcagataaaa gaaatcagga
    25081 gaggatttta caagcagtac aaggaatcca aaacgttgaa atccgggatc tgtatcaaaa
    25141 tagaacaaac aaccagtggg tggagaagta ttttccagat accacgatta ttgataaaga
    25201 agcaaaatta agcgctttag gtaatcggtt gaaagacatc caagaagcta tccaattcat
    25261 acaacaccac ggcgatagcc gtcaaaagaa actccatttg aaacgtcaag aactttcctt
    25321 acaagatttg gaagccaatt attccgaaga aaatttcata aataaattga aagaagtcct
    25381 tgatttaaaa gaaaagtggg aacaactaac tgaagaacgt gatcgtttag aagaagagga
    25441 agaatggctt cttcattggc aacagctgga tattgctcca agtatgtatg caagtaagac
    25501 gaccattttt gagatgggga ctgtgagtgc agcaaatttt gaaagtttca aggaagaact
    25561 gagtaagctt gatgacgttt atatggaaga aatagattat acgccaaaac aagtttatct
    25621 ttcgtatgtt gctttaaaaa aatcagttgc tgttatagaa gaaattgctg ggaaatacgg
    25681 gttcaataaa gaaacgtatc cttatgaaca aaatcctaaa gaaaaactgc aagagaccaa
    25741 gcagcaatta tccattgttc acgagcagca aaagaagata acgacagcgt taggtcagtg
    25801 cagtggctat atccaagaat ttgaatgggt agaagaagtc acacttgccg ttgcagaacg
    25861 agaaaaaata aaagaacggt ttatccatgc gacttatctg atcgttttgc aaggttgggt
    25921 agatgcagaa gaaaaacaga atttgctgca tgtgttaaac gaaaccgtat cagaagaaga
    25981 tatatacgtc agtttcgaaa caccaaactc agaagagatt cagcaggaag tccctactaa
    26041 attgaaaaac catccgcttg tcgaaccttt tgaattgctg actgaaatgt acagtctgcc
    26101 aaaatatgaa gaagtagatc caacaccttg gatgacacca ttttatcttg tcttcttcgg
    26161 gatgatggtc gcagatgttg gttatggttt gctgatgctc attgggacaa tactagctca
    26221 aaaactactt gttctaccac gcgggatgaa acgtttcgta aaattctttg agattctctc
    26281 gataccatcg atcatctggg gattgatcta cagttcattt ttcggacagt cattaccaaa
    26341 agaactgttt ggcatccgct tgcctttccc aatcctatct acgacggatg acgtcaatac
    26401 gattttgatt ttatccgtga ttttcggtct gatacagatt ttggttggat tatttgtggc
    26461 agcaaaggaa aatatcagga gaaaagatta cttgagtgct gtcagtgatg gatttgcctg
    26521 gcaagggatc ctaattggga tcgttattgc gttggttggt gccatgttga taaaaaatac
    26581 agcatttgtt taccttggcg ctgggctatc gatcattgct gccttatgta tcttgatcgt
    26641 tccgattatc caaacgccgt ctaaagtcaa aggtgctgcc aaaggtgcgt ataatcttta
    26701 cggactgacc ggctatatcg gtgatttagt cagttataca cgtttgatgg cattgggtat
    26761 ttcaggcgga agtattggag cggcattcaa tatgctagtt gctttcatgc cgccagctgc
    26821 tcgattcagt gtagggatcc ttttgatcat cgctttacac gcactgaata tgtttttgac
    26881 actgctaagt gcctatgtcc atggcgcacg attgcagtat gtcgaatttt ttgggaagtt
    26941 ttatacagga ggcggacgtg cctttgatcc gctgaaaacg gcggaaaaat acgtcaacat
    27001 caatcacaag aaaaaaaaca ataaataatt ggaggatttt tatataatga tggattactt
    27061 aattagtcaa aatggtggaa tggtatttgc ggtattagcg atggcaacag cgacgatttt
    27121 ttcagggatc ggttcagcta aaggtgttgg aatgacaggt gaggcagcag cagcattgac
    27181 aacaagtcaa cccgaaaaat tcgggcaggc tctgattctg caattgcttc caggcacgca
    27241 aggtttgtat ggattcgtta ttgccttttt gatttttatc aacttaggaa atgacatgtc
    27301 agttgtacaa ggactgaact ttctaggtgc ttctctgccg attgctttta caggcttgtt
    27361 ttcaggtatt gcccaaggaa aagtcgcagc ggcaggtatt caaattttag cgaaaaaacc
    27421 tgaacatgct acaaaaggaa tcatttttgc tgcgatggtt gaaacatacg cgattttagg
    27481 tttcgttatt tccttcctat tagtgttgaa tgcctaagca taaaggagga aaagcagatg
    27541 aacgccattg aaaaaatcat ctctcagatg aacgaagcag cagagcagga aagagcggcg
    27601 ttagaacaag aagagcgaat gaagatcgat caaaactttg aacaaaaacg tacgcaagtt
    27661 gaaacagaac atcaaaaaca aaaagaaaaa caaatcgaat tactcgaaaa aaaataccgt
    27721 cagctaagga atcgccagca agtagaagta cgacaagaaa atttgaatgc gaagcaggaa
    27781 tttttaagac gattgtttgc agatgcggta actgaaatgg aaaattggga tgaatctgaa
    27841 caaatccagt ttatcaaaaa tgcattgtat tcattacctt tgacaggaaa agtagcattc
    27901 attgctggag aaaaatcagc agcctatctt tctcagacgt tgttggatga atggaacaat
    27961 gaacttccct ttatgatggt tttaagtgat gaaactgttg ctgatcaagc gggttttctg
    28021 ataaacgatc aaggagttca gtataatttt cttttcagca gccttgtaca agatatccaa
    28081 ggaacgatga gttttgagat tgccaatcaa ttattcgaat aaggagggat cagtgtatgg
    28141 aataccacga actaaatccc ttgattcgcg gaagagagct tgaacttttg tccaatgaga
    28201 catttgaacg gatgattcaa acaaattctg tcgaagcact tggtgaaata ttgaaatcca
    28261 cgatttacag tccatatatt tatgacggat atgaatatga cttcgaacag aatcttttaa
    28321 aggagcagag ccgtctattt cgctggttaa aggaatcagc accggagcct gaaatcgttt
    28381 ggatctatac gatgcgttat actttccata atctgaaggt tttgacaaaa gctgagatga
    28441 caaatcaaaa tctggaccat ttgtatatcg acgatggatt ttattcatta gaaaccttga
    28501 aggatgccat tcatacgcaa gtttcaaatg aacttcctga aagcattatg gacagtatac
    28561 gagaagtacg tgattatttt gaagagtcta ccattcttca gggaattgac gtgatttatg
    28621 atcgacagtt tttgacagag cagcgacggc ttgccgataa gctaggatat caagaattgc
    28681 tgacagaagt cattgctttt attgacttga ccaatatcac aacgatggct cgaggaatat
    28741 tgcagaaaag aacatatggg ttcatgacga cggtactttc cagctcggga agtatcgaaa
    28801 aagaagtatt tttgaacttt gtagataaag atatggaaga ttacacgaat tttctattag
    28861 agacagatta tgctgatttt ctgcgccctg tcatccgtga gggaaatatt gatctcgtaa
    28921 agcttgaaca gttgaaagat gattatctaa gttcattgta tcaagaagca caaacccagg
    28981 ctttcggacc actgccacta ctagcctttt tgaacgcaaa agaggtagaa agcaaaaacc
    29041 ttcgtttact ggttatcggc aaaagaagcc atttcgaatc agaaacaatc agaaagcggg
    29101 tgagacaagt ctatgacgca taagatcggt gtagttggcg acaaagattc tgttttgcca
    29161 ttcaaattgt ttggctttga tgttcgttat ggtacaacca agcaaaccgt tcgtcaggca
    29221 attgaagaaa tggcaaagaa tgagtatggc gtgatttatg tcacagagca atgtgcagca
    29281 ttgagcccag aaacgatcga acgttacaag gaacaaatga tacctgctat cgtattgatt
    29341 cccaatcatc aaggatcatt agggataggg ctggaagaaa ttcaaaaaaa cgtagaaaaa
    29401 gcagtaggtc aaaatatctt atagtatgaa aaggagatag aaattttgca aattggaaaa
    29461 atcataaaag tctccggacc tcttgtaatg gctgagaaca tgtctgatgc cagtatccaa
    29521 gacatgtgtc tagtgggaga tctaggagtt atcggagaaa ttatcgagat gcgtggcgat
    29581 gtggcgtcta ttcaagtata tgaagagact tctggaattg gtccaggcga accggttcgc
    29641 tctactggtg aagctttatc tgttgaatta ggaccaggga tcatttcaca aatgtttgac
    29701 ggaatccaac ggccattaga tacctttatg gaaatcacac aaagtaattt cttagggcgc
    29761 ggagttcaat tgcccgcatt ggaccatgag aaaaaatggt ggttcgaacc aacagtagaa
    29821 gccggagaaa cggtgagtgc tggagatgtt atcgggatcg tagaagaaac aaaagttata
    29881 aaacataaaa tcatggtgcc aaatgggata aaaggaacga tcaaaacaat cgaaaaaggt
    29941 tcattcacta ttgatgaaac agtatgtgtc attgaaaccg aaaatggtga caaaaatttg
    30001 acgatgcttc aaaaatggcc agtaagacgt agtcggccaa tcaaagaaaa attaaacccg
    30061 gatgcaccca tgatcactgg acagcgagtg attgatacat tcttcccagt gacaaaagga
    30121 ggagcagcag ctgtacctgg tccttttggt gcagggaaaa cagtcgttca gcatcaaatt
    30181 gcaaaatggg ccgatgtgga catggttgtc tatgtgggat gtggcgagcg tggaaatgag
    30241 atgacggatg ttttaaatga attccctgaa ttgattgacc ctaatacagg agaatcgttg
    30301 atggaacgta cggtattgat tgctaataca tcaaacatgc cagtagcagc tcgagaagct
    30361 tctatctata cgggaatcac aattgcggaa tatttccgtg acatgggtta tgacgttgct
    30421 atcatggcag actccacttc acgatgggca gaagcacttc gtgagatgag cggacgtttg
    30481 gaagaaatgc ctggtgatga aggttatcca gcttatcttg gttcacggct tgcggaatac
    30541 tatgaacgat ctggaagagt cattgcatta ggatcagaag gtcgcgaagg aagtataacc
    30601 gcaatcagtg ctgtatcccc gtctggaggg gatatttcgg aaccagtcac acaaaataca
    30661 ttgcgggtcg taaaagtatt ctggggtctt gattccagtt tagcccaaaa aagacatttt
    30721 ccttcgatcg attggataca aagctattct ctttattcaa cagaagtagg gaagtatatg
    30781 gatcatatcc ttcaagctga ctgggcatcc atggtcaccg agggcatgcg tattttgcag
    30841 gaagaacagc agctgaatga aatcgtgcgt ttggtaggga ttgattccct ttcagataat
    30901 gaccgtctga cattagaagt ggcaaaatcc attcgtgaag attatttgca gcaaaatgct
    30961 tttgatgatg tcgatacatt cacttcaaga gagaaacaat tcaaaatgtt gaagatgatc
    31021 ttgacctttg gtgaagaagg acgccgtgcg ttgtcgttag gtgcttattt aagtgagatc
    31081 atggacggta ccgtatcgat tcgtgaaagg atcggacgaa gcaaatatat cccggaaaca
    31141 gaattagaaa aactagatca aattattggt gaaatcaagc aaacgatgca aacgatcgtt
    31201 tcagaaggag gcatgagcaa tgattaaaga atatcgtacg atcggtgaag tcgtcggtcc
    31261 attgatggcg gttgaaaaag ttgctggtgt caaatatgaa gaattaatcg aagtccgtat
    31321 gcaaaatggc gagattcgtc gtggacaagt attggaagtc caagaagata aagcaatggt
    31381 ccaaatattc gaaggaacaa gtggtatcaa tttacgtgac tcttctgtac gatttttagg
    31441 ccatccttta gaactaggtg tatcggaaga catgatcggt cgagtattcg atggtttggg
    31501 acgaccgaaa gataacggac cagagatcct gcctgaaaag catttggata ttaacggaga
    31561 agtaatcaat ccagtagctc gggattatcc agatgagttt atccaaacag gtatctctgc
    31621 aatcgatcat ttgaatacat tagttcgtgg gcaaaaatta ccagtatttt ctggctctgg
    31681 tcttcctcat aaagaattag ctgctcagat cgcgcgacaa gcaacggtac tcgattcatc
    31741 agatgatttc gccgtagtct ttgctgctat tggaatcact tttgaagaag ctgaattttt
    31801 catggaagat tttcgtcaaa cgggcgcaat cgaccgttct gtcatgttta tgaatttagc
    31861 aaacgaccct gcaatcgaac gtatcgctac tcctcgtatg gctttgactg ctgcagaata
    31921 tcttgcttat gaaaagggaa tgcatgtttt ggttatcatg acagacatga ccaactatgc
    31981 agaggcattg cgagagattt ctgcagcacg gcgagaagtt ccaggaagaa gaggatatcc
    32041 tgggtatctt tacacgaact tagctacttt attcgaacga gcaggaagaa tccgcggact
    32101 aaaaggatcg gttactcaaa ttccgattct aacaatgcca gaagacgata aaactcatcc
    32161 aatccctgac ttgactggtt atattacaga aggacagatc atcttgacta gagagcttta
    32221 caagagtggg attcagccac caattgatgt actcccgtct ctttcgcggt taaaagataa
    32281 gggaactgga cccggaaaga caagagaaga ccatgcagct acgatgaatc agctttttgc
    32341 agcctatgca caaggaaaac aggcaaaaga attagccgta gttttaggtg aatctgcttt
    32401 atctgatgta gacaagatct atgcgaagtt tgctgagcgg tttgaaaaag aatatgtcaa
    32461 tcaaggattc tacacgaacc gttcgattac tgaaacactt gatctagggt gggaactgct
    32521 atcgatgttg ccaagaaccg aattaaaacg aatcaaagac gacatgctgg acaaatatct
    32581 gccagaaggg aagtgagttc tgatggcacg attgaatgtg aatccgaccc gcatggaatt
    32641 gactcgattg aaaaaacagt tgacaactgc cacaagaggg cataaattat taaaagataa
    32701 gcaagatgaa ctgatgcgtc aattcatcct gttgattcga aagaacaacc agctgcgtca
    32761 agaaatggaa gagtctatga cagaagcaat gtctgatttt gtattggcaa atgcttcgat
    32821 cacggaacct ttcatcgaag aattatttgt tttgccggcg gaaaacgttg atttgtctat
    32881 cgtagacaaa aatatcatga gtgtaaaagt acctgtgatg aattttgaat atgatgaaca
    32941 gctgacagac acacctttgg aatacgggta tttgaattcc agtgccgaac tggatcggtc
    33001 aatcgatcgg tttactaccc tgcttccaaa acttttggaa ctgacagaaa tcgaaaaaac
    33061 ttgtcaactg atggcgagtg aaatcgaaaa gacacgtcgg cgagtcaatg cattagaata
    33121 tatgacgatc cctcaattag aagaaacgat ctattatatt cgtatgaagc tggaagaaaa
    33181 cgagcgcgca gaagtaacaa ggttgatcaa agtaaaaaat atgggtacca ctgactaagt
    33241 ggtatctttt tttaaaagag gaaaacgatg attaaacaaa gcaaacaaat aaagaaacgt
    33301 ttgccggctg ttcagcttct cgcattagga ttttttctcc tgattttagt tggcggcggc
    33361 ctacttaccc taccattttt tagtaatagt gggcatggga caaattttat cgatgcgctt
    33421 ttcactgcaa cttctgctgt ctgtgtaacg ggattgacaa cattgaatac agcagagcat
    33481 tggaacagtg ctggtcaatt tttgatcatg cttttgattg agatcggcgg tcttggtttt
    33541 atgatggttc cgattctatg ctttgcattg ataaagaaga aagtcagtct cagcacacgt
    33601 attatattaa aagaagcgct gaatctagaa gaagtatcag gtgtgatgaa gctcatgctt
    33661 tacatcctaa aattggcctt tgtgatccaa ttgattggta cggttgcttt atcttttgta
    33721 tttgtgcctg aatttggctg gttgaaaggt atctggtaca gtttgttcca tgcaatctct
    33781 agtttttgta atgcagggtt tgatttgtta ggagacagtt tagccaatca ccagcaaaat
    33841 gtttatttga tcatggtggt atctgctttg atcatttctg gtgggttagg cttcatcgta
    33901 tggcgtgatt tactggaata tcatcagaaa aagaagatga cgattcattc aaaaatcgca
    33961 ttaaccgtta caggtaccct tttattaggc ggattcgttg tgttcttttt cacagagcgc
    34021 aatggggcaa cgctagtgga agggaatatg tttcaacgat tggcaaacac cttttttatg
    34081 agtgtgacac ctagaacggc tggatattat tcagttgatt acctccatat gagccatgca
    34141 ggtctgattt taacgatgtt tttgatgtac atcggcggaa catctgggtc gacggccggc
    34201 ggattgaaaa caactacgtt aggtgtgttg ctgatccaga tgcgttcgat gttgaaagga
    34261 cgtacgagag cggaagcatt tggccgtacc atccgacaag gcgcagtgtt gcgggcactt
    34321 actttatttt ttgttacgtt gacgttgtgt gtagtcgcca tcatgatttt atcggtgact
    34381 gaaacgatac ctaaaacttc tggcatcgaa tatattgctt ttgaggtctt ctctgcgttt
    34441 ggtacagtag gtcttacgat gggattgacg ccagacttga ctgcaatagg gaaattgatc
    34501 attatagcgc ttatgtacat tggacgagtt ggtgtattga ctgtggtctt ctctctgatg
    34561 acaaaagcac aaagaacaca atcaaaatac aaatatccag aagaaagtgt catgattgga
    34621 taatttcatc ttctgacaga aacctagtga tttagtaaaa taagaaaata aaataatttt
    34681 tcttgcttct tggatacttt aagtgcaaaa tgttcgttta ttttttgagg cactacccgt
    34741 tgttgaacct cgggtagtgc ctctttttta ttgaaaggaa tcaataagat ttgattcctt
    34801 tatttatcaa taggttataa ttcatatttt tttatttttt cttttacgat acttagtcca
    34861 gaagctaaaa tttcaaggtt attagaggat agggaaattc gaacgaatgc atctggttgc
    34921 gcacgtgtag taaagcgtct ggaatgataa agtcggatac cgttagcaag aaagtcattc
    34981 tcaattatct tagggtcaat ctcaggatca actggaagaa ttcgatagta agggacacga
    35041 ttcgttggtt tttctacttt gaaaagagaa tcaaaaagct gattggctct ttttgtaaga
    35101 cttaatttag tttctaagat cctatttgct tcatttgaat ggaggatttg agtgatgact
    35161 tccacatcta atccagaagt tttcacattt atattaaaaa ttgcttgttc tatcttgctt
    35221 tggacatgta ctggaactac aagataagca actcgcaatc cagaacaaat aaattttgtc
    35281 attccagcta agtagattgt ttggttaggt aataaatttt ggaaaggagg aatgatcgta
    35341 tgttgatagt aagttgttaa aaatgaatgg atatcatctt caataaccca tagaccttct
    35401 ttttcaatca cttcggctaa ttgctctcgt cttttgtgag agatttgaaa ccctatagga
    35461 ttattacaag aaggcataag aaagacacca tgaatcttct tttttctaca ttcctgaatc
    35521 aatatatcag gtcgcattcc ttgctcgtca aaatcaattg gaacgatctc taaatgatat
    35581 aactgagcca attcaatgaa attagcgtat gtatatcggt ctgttgctat tctatcacct
    35641 ggagagaaaa gtgcagaaag tgtgatagcc aatccatttt gaacaccgga aacaatagat
    35701 agagattcta aggacggaat gactccttga gtttttaacc aatttgctgc agtagtcaat
    35761 tgaaaatttg tgcctttggg atcttggtaa ttaagcaaac caacaagatt tgggttatta
    35821 ctagttgatt gaataaacgg gacaacgaat ttattacatt cttcaaaaga actaaccaga
    35881 ccaaaatcaa tgatatgttc ggttacttca ttggtagaga tggtgatgga ctcaaagaca
    35941 ttgggagaaa taaatgtgcc acttccaata ttcgtataca gcaaaccttt ctcaattcca
    36001 tatttataag ccttgccaac ggtagtaaaa tttaaatcta aataatcagc tagctctctt
    36061 tgggagggta actgagtgtt tttctgtaat ttattcttta aaatatcttg ttctaattgt
    36121 tgaatcaacg atcgataata aggacgcttc aatttttgtt tgtctggtcg ccaagttaac
    36181 gggtaattgt cataagaatt aataggcatt ttctccactc cttaaaattg tataccatac
    36241 aattttacca ttgatgtgcg ccctgcatgg gtgaaaactt ggtggtgaaa gtccactaca
    36301 ggcttggcag taggaactgt tagcgaaaga caaggcggca atcgtgaggt tgtggctgaa
    36361 ggaagtcgga cgcaaactcc tgaactgacg aacagaaact agatacaagg ctgaattagg
    36421 tcggataagt cttcatcaca agacaaagtc caatactgcc cgaaacctat acagtaaatc
    36481 tagcagttac atgggaggaa agttttcact cttaccgggg gagatctcat gagggtgttg
    36541 tgggcgttga atgaaaacaa accagcacaa cgatcatggc gacatgatgg tgaatcatga
    36601 gaagtcagcc gaggtcatag tagtaacgac actgaccgtt acgaaggact gaacaataag
    36661 aatcttgaat aatttaggag gtgtgagaag atgcgacgac cgcagaaaac aagaaaactt
    36721 ggctatcatc aaagagctag tgtggaacac gaagggtatg atgaagtgcg cagcacttct
    36781 tgcggtgaac agaggaaaca agatggtgta gaaaatctgt ttgggagaat tctagatcgg
    36841 aataatctca atcaagcgta tcttcaagta gtcagaaata aaggggcggc tggtgcagat
    36901 gggatgaccg tcgatcaact gcttccttac ttgaaagagc accgcgagga attactatcc
    36961 aatttacgcc aagggaacta taaacctcag cctgttttgc gagtagaaat accgaaacca
    37021 aacggcggtg taagaaaact aggaattccc acggttattg atcgtatgct tcaacaggca
    37081 atcaatcaag tgttacaacc gctatttgaa ccagaattct cagataatag ttatgggttt
    37141 cgcccaaatc gcagcgcaca tgacgcagtg aagaaagcaa aatcttatta tgaacaaggt
    37201 taccgatatg tggtggacat tgatatgaaa gcctactttg acaccgtaaa tcacgataag
    37261 ctgatgtatt tcattgagcg gaaagtaaag gacaaacaag tcctaaaact gatccgtcaa
    37321 tacttaatga gtggtgtaat ggaaaatgga ctcatcacaa caactgaaga aggaacgccc
    37381 caaggaggca atctctctcc cctgttaagt aacatctacc tgaacgaatt tgatcaagtg
    37441 ctagaaaatc taggacataa atttgttcga tacgccgatg actgcaatat ctatgttaga
    37501 agcaaacgag caggacagcg tgtgatgaag aaatccattc aatttctcga agaacaacta
    37561 aagttgacgg taaatcgaga aaagagtgcg gtaggaagtc cattgaaacg aaagtttctt
    37621 ggattctgca ttctgccaac aaagaaaggt attaaaattc gccctcacgc aaaagcaaaa
    37681 cagcgtgtga aaagcaagct caaacaactc actaaaagaa accgtggaag aagtatccaa
    37741 cttattttaa aagaaatcaa gcaactaatg actggttgga tcaactacta tggaattggt
    37801 gagatgaaag aattcatgag ggagttaaac ggttggttga agcgaagaat cagacaatat
    37861 atttggaaac aatggaagaa tccaagaacc aaaagaaaaa atctgattcg tttagggatc
    37921 gataaagcga aagcgtatga atggtcaaat acaagaaaag gtatttggtc agtctccaaa
    37981 agtcatattc ttcatcgatc attaacagac aaagaactgg cccgccaagg atatgaggac
    38041 atctccttga agtaccagtt cgtacactca acttattgaa ccgccgtata cgggtccgta
    38101 cgtacggtgg tgtgagaggg gcggaaggtc agtt
//
LOCUS       DAZKLM010000016        37083 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3764
            SAMEA115706913-rid22582563.denovo.016, whole genome shotgun
            sequence.
ACCESSION   DAZKLM010000016 DAZKLM010000000
VERSION     DAZKLM010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706913
            Sequence Read Archive: ERR13196647
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 37083)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37083)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287823.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287823.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,004
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,849
            CDSs (with protein)               :: 2,849
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 41 of 96
            Pseudo Genes (incomplete)         :: 52 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 15 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37083
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706913-rid22582563.denovo.016"
                     /isolate="VRE3764"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..386)
                     /locus_tag="ACG842_000764"
     CDS             complement(<1..386)
                     /locus_tag="ACG842_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0247326.1"
                     /translation="MNFLVVLKDTLIERSTFLYVQLQENKTLLHFSPEFHLEGLTLGE
                     IYQAEGLSAVLHFFKVELELPVKDYIELSLESWDRAVVELFPEGLMIDTNDGKIHLTA
                     AELQKQMRYQIDDENSFSIFRRQQKIL"
     gene            complement(571..969)
                     /locus_tag="ACG842_000765"
     CDS             complement(571..969)
                     /locus_tag="ACG842_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HFQ0247327.1"
                     /translation="MKDLVALNLHMIVTIVDGGMGEDVIDFSKEAGAAGGTILHGRGS
                     GVHDSGKFLGLDISPEKDIVLTLVPDSLTNKVMDAIGQGINIDIPGNGICFSIDVDKV
                     IGITKIHQYREIIGMQELLDENNDREKSES"
     gene            complement(966..1739)
                     /locus_tag="ACG842_000766"
     CDS             complement(966..1739)
                     /locus_tag="ACG842_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HFQ0247328.1"
                     /translation="MSFWQQVIDGIEIVTMEVILAVAPIVILFVLMNIISFRMRKKRF
                     FDIMKGFLITTIGLILFLHGVNMAYVPVGQHLGSAIAGLPYNYILVPLGFLMGFLVGF
                     AEPAIHVMIKQVEELSEGRIRSKIMLMVISIGIGAAVGLSMWRLLVGFSLYYFLIPGY
                     GLAFILGRKVDKIFLAMAFDNGGVATGPMCSTFILSMSVAIAAQIEGRDPIIDGFGVV
                     AMIALTPILSTLVLGYIYKRKDEKDRIEKERLQKLGESK"
     gene            complement(1736..2419)
                     /locus_tag="ACG842_000767"
     CDS             complement(1736..2419)
                     /locus_tag="ACG842_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HFQ0247329.1"
                     /translation="MLKNFKEVIAAILPMTVLIVILTFVFAPLEGEDLASFLVGAAIM
                     MIGMTLFLFGADYSMMEVGDLVGKYMIKKKSLTVLVSLGFAIGIVITIAEPSVQVLGQ
                     QVYDISDGEIGRVLLIGIVSVGTGVFLAFALLRVVFKLSYYQLMAIGYIGVLIASFFT
                     SSEFMPIAFDSGGVTTGPVTVPFILALAGGMTSMIKQKKNENDSFGMVGIASLGPILA
                     VMILGVIFQ"
     gene            complement(2586..3473)
                     /locus_tag="ACG842_000768"
     CDS             complement(2586..3473)
                     /locus_tag="ACG842_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0247330.1"
                     /translation="MKNMVEKETIERPNVKKQALELRSTIGSVLKYTALSILSFISIF
                     PFVWMVLGMTNTPVDITSGKIKIGSNLIINFQNLFSNELNFTRSLWNSAMIALVTTVF
                     ALLISSMAGYGFEIYQSKRKDLIFNILLLSMMVPFAALMIPLYRMFSQLNGTVLGINS
                     FLVVILPSVSTAFLIFFFRQNVKSFPKDLVEAARLDGLKELSIFFRVYMPTAKNTYAA
                     AAIITFMSSWNNYLWPLVALQSPDKRTVPLVLSAMGASYTPDYGMIMAGLVIATLPTA
                     IVFFVLQKQFVQGMIGSVK"
     gene            complement(3470..4339)
                     /locus_tag="ACG842_000769"
     CDS             complement(3470..4339)
                     /locus_tag="ACG842_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0247331.1"
                     /translation="MKQKDLFKNGHAFLILPVILISIMVFIPMVLALITSFQSGPPVN
                     MTFNGLGNYQRMLTDATFKKAFFNTFLYLLVQVPIMLFLALFISNLLNDKKLKYKGLF
                     RTAIFLPCITSMVSYSLIMKSLFSQSGLVNNLLLGIGMIDTPIGWLTHPIWARVLIIL
                     SITWRWTGYNMIFFLSGMQNIDSEIYEAASIDGASKWQQFIKVTIPNLKPIILFTSIT
                     STIGTLQLFDEVQNITGGGPANATTTLSQYIYNLSYKFTPNFGYAAAVSFVIVSFIVI
                     LSIIQTKVGGESR"
     gene            complement(4476..5762)
                     /locus_tag="ACG842_000770"
     CDS             complement(4476..5762)
                     /locus_tag="ACG842_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFQ0247332.1"
                     /translation="MNIRKLIAGVVCLAAASGFLTGCGSASSTADKEEKVTVWAWDET
                     FNIKAVNEAKKVYENEETEIEVVTMSQDDIVQKLNTALASGNTEGLPNIVLIEDYRIQ
                     GYLTSYPDAFANLSAIVKEDHFAPYKFAVNKVGDKIYGVPFDSGVTANFYRTDLMAEA
                     GYTEEDMENLKWDEYIQVARDVKEKTGKKIAEVNPSDLGRVRMIMQQAGEWYTSEDGE
                     TVTIQDNASLKYGLELFATLLKEDLVEQTSDWNAGVTAIQSGAVASSPTGAWYSSTIQ
                     GAENQSGKWKIAPIPALPENLQKAQASNLGGAGWYVIKGVAGEDSAKDFLEATFASNE
                     ELMGTLAKEIGLVSTMLSANEQEAYQEASEFYSGQKIFDDFSTWTSEIPEVNYGHETY
                     AIEAVVAEALHRIINGEETDKVLADTQKQVEAQLAN"
     gene            6035..6613
                     /locus_tag="ACG842_000771"
     CDS             6035..6613
                     /locus_tag="ACG842_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFQ0247333.1"
                     /translation="MPIRVILIDDEQTILDGMKQLINWSEHGFQLIGTFSSPLKALDF
                     SLVHAIDIVVTDVMMPELNGIELSRLLKQQQPETQILILSSYDEFDMVKDSFKEGVAD
                     YLLKPRLSPDFFLNSLTAVSQKIKKKPNKSPITNRYEQICENLSQQIAGQTRPLLDPL
                     LFEHTLFFCYIPKNGHSVQNKALNKNSRSPIH"
     gene            6613..7542
                     /locus_tag="ACG842_000772"
     CDS             6613..7542
                     /locus_tag="ACG842_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0247334.1"
                     /translation="MDSDDSFTIYPVSTFSEESGYVINTDSKDTLRKFVQKFRQEVFP
                     GDFFVVSDSFAIQDINEIFQEIRNCTKGQRFYHKGKNLAYQHELLPLLTNHEQQASSY
                     LTKIVHKDYALAVKELENFFTTILEQPIDPAYLKHEAINRFYALLNALADEHVGNEEL
                     SLLKIALPSLLADAEYFEDFSILVKETLEKLTHFSANAKKDNFDILTLIYEFVQENYE
                     KEINLQLLSEKYHFSYSYLSAIFTEKFGINFSKYLKKVRISKAKLFLTETRLTLTEIC
                     EKVGYTELGYFSRVFKEETGMTPSQYRKGTFVK"
     gene            7539..9275
                     /locus_tag="ACG842_000773"
     CDS             7539..9275
                     /locus_tag="ACG842_000773"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFQ0247335.1"
                     /translation="MRKKIKEHELFAKLLFILFIGLFLQAIVISLFIYHRSRDAYIQL
                     FNQSNDVVLKKIQSEFESLNDTIENTLAAFDSNPAVKSYFSNDPAQHMEQLQQLRTIQ
                     KMNDSLSKIHPMIDYDVLIFGENGRTFVGNDMLTAVSADSFFQSAIAQRVNERAADTQ
                     MLFAHHGLTLRDKKAPSVFFVKKLKNTLNHVYGYAVLSISSKQLANLFQSVVNPEISK
                     ISFMNEEQTIIASNEENTIGSRSMIFKKLTVGETTISNGQQLTRLPLYRQDCTLLSQV
                     DLHSLSNQMGVIFPIILYNIVTLAFAGSLVFMYLDRHTKSIYRLIDSLKRLEEDGLKA
                     TVPVQGSHEVQLLGNTINQLLSNAQHHYEQTIQDEKKKRSLEIQTMQAQIQPHFIYNT
                     LTSLKFLIWQKKTDEAVQGIDSFITLLRSTIGKKEEVIPVKEEIKSVQSYIDLLSMRY
                     GERISAKIMIPDELQSLSMPNMILQPIIENAYLHAFHNKTQGFITVYGKTKNQLLLFE
                     VIDNGDGFDTENTKQKNDHFSGIGISHVDERIRLMYGDAYGLTIQSVIGTGTTVTICL
                     PLLPKNQSEYQK"
     gene            complement(9338..10174)
                     /gene="pheA"
                     /locus_tag="ACG842_000774"
     CDS             complement(9338..10174)
                     /gene="pheA"
                     /locus_tag="ACG842_000774"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HFQ0247336.1"
                     /translation="MNVSYLGPESSFTYQAACQLFPTEQLTAYASIPVCLRALFRKQV
                     DLAVVPVENSLEGAVHHTIDLLSKHPEVEVKSEIVLPIKQQLLGNPATKITKILSHPQ
                     ALAQSQQFLETHYPNVPLVATESTTAAAMYVAEHPKEDAAAIASLETAQHVGLEILAE
                     NIQDNELNQTRFWIVGDRKMTSQQSAPVKMSVILTLPANRPGMLHKMLAAFGWREINL
                     SKIESRPLKTSLGEYFFVIDLLLDRPMTLVENALQEIKMLGGESQILGCYPVLTVEET
                     RR"
     gene            complement(10171..10680)
                     /locus_tag="ACG842_000775"
     CDS             complement(10171..10680)
                     /locus_tag="ACG842_000775"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HFQ0247337.1"
                     /translation="MASILLIGFMGAGKTTIGKGLAQCLQKEYVDLDTKIEEHIQLSI
                     AEYFHYYGEKSFRKVESDILRKLSNEDKIIATGGGIVQSAENRRFLKTQPIVLYLEAE
                     ADCLVDRIQQDETSIRPLALGKTREEIKALLAQRLSWYEESATHRINTTNQTPEKIID
                     TIIERIKSI"
     gene            complement(10673..11968)
                     /gene="aroA"
                     /locus_tag="ACG842_000776"
     CDS             complement(10673..11968)
                     /gene="aroA"
                     /locus_tag="ACG842_000776"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HFQ0247338.1"
                     /translation="MQLLQQIHGLRGTVRIPADKSISHRSIMFGAIAEGTTTIQNFLR
                     AEDCLSTLHAFQQLGVEIEEEEEVIKIHGRGSHSFVQPTESIDMGNSGTTIRLLMGIL
                     AGQPFTTTLFGDASLSKRPMGRVMEPLREMGADLQGEENDQYLPITVTGTRSLSPIRY
                     NMPVASAQVKSALLFAALQAEGTSVIVEKERSRNHTEEMIRQFGGRITVEDKTIIVTG
                     PQKLTGQQITVPGDISSAAFFLAAGLLVPESQLLLKNVGVNPTRTGILDVLEEMGAAI
                     TQTNHNEQNQSADLSVKTSHLKKATIGGEIIPRLIDELPILALVATQAEGITIIKDAE
                     ELKVKETNRIDAVAEELQKMGAKIKATADGLIIHGPTPLHGAKVSSRGDHRIGMMLQV
                     AALIADGPCELEGAEAVSISYPAFFDDLAELVSGGEAHG"
     gene            complement(11981..13072)
                     /locus_tag="ACG842_000777"
     CDS             complement(11981..13072)
                     /locus_tag="ACG842_000777"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HFQ0247339.1"
                     /translation="MAQHVFIVGLGLIGASMALCIRQTNPELIVVGWDSQSKTREQAV
                     EQQIVDYVASDFETGAEQADVILLAVPVRTTLAYLDVLERINLSDHVIITDVSSTKQQ
                     VVTSAEQKNLRFVGGHPMAGSHKSGIQAADAKLFENAYYIFTPLEKTKKDVEKLQELF
                     CGTKAKYVTLTAREHDRITGMLSHFPHILAAGLVNQAEQFNQEYPRAKQLAAGGFRDI
                     TRIASSDPVMWTDILLSNKQILLERLADWQQEMTQIAEWIMTENQSEIFSFFNRAKES
                     RDQLPVHKQGAIPAFFDLFIDVPDEPGVIAEVTGLIGKAGVSLINLKIQETREDILGI
                     LQISFKNQQDLLQAKRIIMSNTSYHCWIK"
     gene            complement(13089..14255)
                     /gene="aroC"
                     /locus_tag="ACG842_000778"
     CDS             complement(13089..14255)
                     /gene="aroC"
                     /locus_tag="ACG842_000778"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HFQ0247340.1"
                     /translation="MRYMTAGESHGPELTAIIEGLPAGLPLSEEEINHELARRQAGYG
                     RGGRMKIEKDRVRITSGIRHGKTIGSPVTLVVENKDWKNWTTVMSVAEVPEKDQSLRR
                     VARPRPGHADLVGGMKYHHRDLRNVLERSSARETTMRVAIGAIAKKILEELDIIVAGH
                     VICLGGIQAEIPGNISIQEIKEISEASEVRVVDPAVEQRIKDLIDQTKKNGDTIGGIV
                     EVLVGGVPAGLGSYVQWDTKLDAKIAQAVVSINAFKGVEFGVGFTSGSLPGSEVMDEI
                     LWEEERGYFRRTNNLGGFEGGMTNGEPLVIRGVMKPIPTLYKPLQSVDIDTKEPYKAS
                     VERSDSTAVPAASVVAENVVATVVATELLSKFPSDSLVELKAAVKEYRDYLKHY"
     gene            complement(14257..15321)
                     /gene="aroB"
                     /locus_tag="ACG842_000779"
     CDS             complement(14257..15321)
                     /gene="aroB"
                     /locus_tag="ACG842_000779"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HFQ0247341.1"
                     /translation="MLTVHVDNQKYDIHIEKGALEQAGSWLQTIWRPQKVAVITDTNV
                     APLYAEKIMFQLRQANFEPVLSIVPAGETSKSLEQAAELYDFLADNDFTRSDGILALG
                     GGMVGDLAAFVASTFMRGIHFVQIPTTLLAQVDSSIGGKTAVNTNKAKNLVGTFSQPA
                     GVLIDPEVLKTLPVRRLREGIAEIIKSAAIADATLWKQLNELKDEKDLLEHAVPIITA
                     TLEIKRKVVEEDVFDQGNRLILNFGHTIGHAIENTAGYGVVSHGEGVAIGMVAITSHA
                     EKIGLSPTGSTAALERILQKFHLPITYDLSHPDELFHAITHDKKARGSQLNIILLEKI
                     GQAKIVPVPTETFKDYLGGL"
     gene            complement(15354..16376)
                     /gene="aroF"
                     /locus_tag="ACG842_000780"
     CDS             complement(15354..16376)
                     /gene="aroF"
                     /locus_tag="ACG842_000780"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HFQ0247342.1"
                     /translation="MIVIMKKEATEEQIKKVVDHIEKEGFKVSIDQGTERVVIGLKGD
                     TRSLQEGAFTRYEGVENAVRILNTYKLTSREFHPGNTVVDVDGVKIGDGSFVTMAGPC
                     SIEGLDQIRECARMAKAGGAKILRGGAFKPRTSPYAFQGLEEEGLKYIRQAADEFGMK
                     VITEVMDEGHIDMVAEYSDILQIGARNMQNFKLLSAVGKTGKPVGLKRGISGTINEWL
                     NAAEYIAVEDKSPVIFIERGIRTYETATRNTFDLSAVPLMKKLTHFPVIVDPSHGTGI
                     WELVPPMARAGVASGADGMIVEIHPDPANAWSDGPQSLNEKTYSRMMKEVAIMKEAME
                     KIQTLD"
     gene            complement(17035..18009)
                     /locus_tag="ACG842_000781"
     CDS             complement(17035..18009)
                     /locus_tag="ACG842_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase small subunit"
                     /protein_id="HFQ0247343.1"
                     /translation="MKNTVNKSHMDTEKVAIVFGDCTLGVKSGNTHYIFSYTRGGLES
                     LNKNGKEWLYRETLPVFWRATTDNDRGSGFGFKSGMWLGAGLYPKVKEIEVLVDNKKI
                     DLPIAPVNNQYSSNEYAETVEIIFTLEINTVPKTQVTISYQIAGEGKMLVNMHYYGQK
                     GLPELPALGLRFIMPTKAKFFEYEGLSGETYPDRMAGGVEGIYKVAGLPVSPYLVPQE
                     CGMHMETKWVELTRNQTLDNTDKDQEDFSLRFEQQDTPFAFSALPFKAEELEQATHIE
                     ELPPERRTVLTIFAKVRGVGGINSWGADVESPYRISSEEDHEIGFIIQ"
     gene            complement(17990..19873)
                     /locus_tag="ACG842_000782"
     CDS             complement(17990..19873)
                     /locus_tag="ACG842_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2"
                     /protein_id="HFQ0247344.1"
                     /translation="MKANIKWLDDPQVFRVNQSNAHSDHVCYATREEVETTSSLKQSL
                     NGSWRFCYSPNPSVRPKTFYKKDFDACSFDEITVPSHIELANYDKIHYINTMYPWEGH
                     VFRRPPFTTSPEKEAEGIFSAAKDNPVGSYLTTFRLEEGLKGKQISIHFAGVEQAMFI
                     WLNGHFIGYAEDSFTPSEFDLTPYLEEENILAVEVYKRSTAAFLEDQDFFRFFGIFRD
                     VFLVARGQNHLEDLWIKPRLDDKCQNGKLEVDLSFEQVAKNSQLNVAILDPDGKEIYQ
                     QQYPLKEQMTIRIDGFENVALWGHFQPNLYKLELETIDEEKRVTEYIDYSFGFRKIEI
                     KDKIIYFNGKRMIINGVNRHEWHPKKGRAITIEDMEKDLAIIKNAKINGVRTSHYPNQ
                     ITWYYLCDQAGIHVMAETNLESHGSWQKMGAFEPSYNVPGAVPQWQAAVLDRAKTNFE
                     TLKNHPSILFWSLGNESYAEENIRQMNDYFKKVDPDRLVHYEGNFPNPAFEDSISDVK
                     SCMYAPPEEIIDYLENKAEKPFILCEYMHDMGNSLGGMDSYMTLLDRYEMYQGGYIWD
                     FKDQAIQVTDPITGREVMRYGGDFDDKPSDYEFSGNGIVFADGQEKPAMQEVRYYYEK
                     YSK"
     gene            complement(19891..22116)
                     /locus_tag="ACG842_000783"
     CDS             complement(19891..22116)
                     /locus_tag="ACG842_000783"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HFQ0247345.1"
                     /translation="MDITHRKTDKNFILFDEKSRVFHLRNTFLSYLIKIEESNVLAHI
                     YFGKPVKQYKDNKNYPRRDRGFSGNVPLNPDRSLSKDTLPQEYSSHGSMDFRTPASII
                     QRKNGSDLLDLRYDSHYITDGKPEIEGLPQTYVMDKSEAQTLVISLKDRETAIYFDLF
                     YTIFTDRAVITRSVKIRNETGETIKLEKAASFQLDFAHTRRFDEVIALPGAHVNERQI
                     SRQSVLSGTKVFESRRGTSSHHMNNFIALVHHHTTENTGEAIGLQFVYSGNHSFELEK
                     DQINQLRVVGGINSHRFSWELNAGQSFQTPEMILSYSSQGLNKMSQIHHELLRERIAR
                     GRHQFAERPILVNNWEATYFDFNSEKIKAIIDEAKELGIEMFVLDDGWFGKRDADNSS
                     LGDWFEYEGKLTNGLREIADYAHSKGLKFGLWFEPEMISVDSELYRTHPDFLMQEPGR
                     MPSASRSQHVLDFTRLDVRQTIEKQMRKILDTIPLDYIKWDMNRSLSDVYSITLDPQR
                     QGEVAHRYMLGLYELLEHLTTDYPEILWEGCSGGGGRFDAGFIYYMPQSWTSDNTDAV
                     ERMKIQYGTSLAYPISSITAHVSAVPNHQTDRSTSLKTRGETAMSAVFGYELDLTKLS
                     PEEEKQVKDQIISYQTIRPVIQYGHYYRLASPFEENIAAWMFVSPKQDEAIVFLGRIL
                     ASAQPAFHEVYLMGLDDEALYQEQTSKRIFSGAELMTVGLYFPDFQGDFQTELLHFKK
                     L"
     gene            22231..23265
                     /locus_tag="ACG842_000784"
     CDS             22231..23265
                     /locus_tag="ACG842_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFQ0247346.1"
                     /translation="MVGIRDVARKANVSPATVSRVLNNDQTIHVAQTTRQRIVEAAAA
                     LDYDISTRKYIKKRMPSIGVISTIGKASEEQDIYYKHLRVGMEEEARRLHLGMNRIYN
                     LSDNPKQWEDLDQLGAVIVVGTVTKESITALLKQNMQIVVVDNPDIQQEVDMVYADLE
                     RMTKTVLELFLENGHKRIAYIGGYKVDMDEFGGKQISINEKRMRAYQHFMIDHHLEKY
                     MSYHLGGWTEKQGEVLATNLLENESPLPTAILVGSDPMAIGVYRALKQKNIQIGEEIV
                     VASFDNIETIANLSPGLTSVQINAKSIGQAAVRMALERIDGIRDEPIILTYPARLIVR
                     ESFIPRTKRY"
     gene            complement(23332..24345)
                     /locus_tag="ACG842_000785"
     CDS             complement(23332..24345)
                     /locus_tag="ACG842_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFQ0247347.1"
                     /translation="MKKKILKFIGWFFGIILGIAFAIFLAFQLSPRPGAFLINHMFSN
                     TVQITDKKTFDQAEKNVAKKTDLTYESKYKENTYDIYYPKNSKGPVPVLFWVHGGGFV
                     GGDKSGVKEFATKLVSDANIAVVAMNYELAPDSEYPNQVLQVNELIKDLLAEEKDDKL
                     LDMEQLFFGGDSAGSQIALQYAAIQTNDAYAKQMKIKQLLPKDSLKGTISYCGPVDLK
                     LTAQQHSDDRFMKFFVKTVAWSLIGTKDWKTSEQLKEASVADHVSKEFPPTYITDGNA
                     YSFQEQGQALENRLTELKVPVQSLFYTDTKKEITHEYQFDYTLKESQNCYQQTLDFVN
                     KYK"
     gene            complement(24423..24611)
                     /locus_tag="ACG842_000786"
     CDS             complement(24423..24611)
                     /locus_tag="ACG842_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HFQ0247348.1"
                     /translation="MEEHQQVIDRIYEATRYLPLDRLCLSPQCGFASTEEENVLFEEQ
                     WKKFLLSKALQKKFGENH"
     gene            complement(25051..25179)
                     /locus_tag="ACG842_000787"
     CDS             complement(25051..25179)
                     /locus_tag="ACG842_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HFQ0247349.1"
                     /translation="MDQKEKMKELIKKKQGGGRSKQMETPKNDRKNMRKGPKIFNK"
     gene            complement(25182..26555)
                     /gene="rlmD"
                     /locus_tag="ACG842_000788"
     CDS             complement(25182..26555)
                     /gene="rlmD"
                     /locus_tag="ACG842_000788"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HFQ0247350.1"
                     /translation="MTEQQIVKKNERYTVEIVDLSYEGMGVAKIDGYPLFIENALPGE
                     EVEILVVKAGNKFGYGKVETFITQSPDRQPVENNALLRIGIAPLAHLKYEQQLVFKQK
                     QVEHVMAKIAKMPEVPVLPTVGMAEPFGYRNKAQIPVRRIDGQLATGFYKKNSHDLVP
                     IEDFYIQDPVIDQAIKVVRDILQRFQVRGYNEEKNEGQLRHIIIRRGHYSHEMMVVLV
                     TRKEKFFKSKEIAEAIHEELPEVVSVIQNINEEKTNVILGDKEKVLYGRDYIEDQLLG
                     KTYRISAKSFYQVNTQQAEYLYQTAIEFAELDKKDIVIDAYSGIGTIGLSLADRVDKV
                     YGMEVIPEAIEDAQFNALNNKIENVHYEVGKAEKVMKKWQEKGIKPSVIVVDPPRKGL
                     DHHFIESAIEMSPERIVYISCNPATFARDAKLFAEAGYAVKKVQPVDLFPQTHHIELV
                     ASFVKGE"
     gene            complement(26666..27721)
                     /locus_tag="ACG842_000789"
     CDS             complement(26666..27721)
                     /locus_tag="ACG842_000789"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HFQ0247351.1"
                     /translation="MKKARVIYNPTSGKELLKKNLADILQALEEAGFEASAYATTPEP
                     DSAKNEACRVAELGFDLVVAAGGDGTINEVVNGIAPLEHRPKMAIIPAGTTNDYARAL
                     KIPRDNIKAAAEVIKKHQTVKMDIGLSDNSYFINIAAGGYLTELTYEVPSELKSIFGY
                     LAYLAKGAEMLPRVKPIKMRLKYDEGEYIGNASMFFLGLTNSVGGFEQIAPDAKLDDG
                     KFSLIIVKTANIFEILHIAALMLNGGRHVDDPRIIYTKTSSLFVETEDDINRPVMINL
                     DGEYGGEAPMHFQNLHQHIEFFANTDQIPDEAITGTDEEELEVVSKEFVKEVERLTDE
                     DIDGDGKIADETPNQKN"
     gene            complement(27731..29161)
                     /gene="gatB"
                     /locus_tag="ACG842_000790"
     CDS             complement(27731..29161)
                     /gene="gatB"
                     /locus_tag="ACG842_000790"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HFQ0247352.1"
                     /translation="MNFETVIGLEVHVELKTNSKIFSPAPAHFGAEPNSNTNVIDWGY
                     PGVLPVMNKAALEFGMKAALALNCEISKDTHFDRKNYFYPDNPKAYQISQFDQPIGHD
                     GWIEIEVEGKKKKIRIERVHLEEDAGKNMHGIGGYSYVDLNRQGTPLIEIVSEADMRS
                     PEEAYAYLEALRSIIQFTEVSDVKMEEGSMRCDANISLRPYGQEEFGTKAELKNLNSL
                     SFVKKGLAFEEKRQAKVLLSGGEIQQETRRFDETTNKTVLMRVKEGSSDYRYFPEPDI
                     PKFVIDDEWIQKVKASLPEMPASRRERYIREFGLPEYDAMVLTLTKEMSDFFEAVLEN
                     GADAKQASNWLMGEVSAYLNSEKLELAETKLTPENLAGMIRLIADGTISSKIAKKVFR
                     ELITNGGEAQEVVEKNGWVQLSDPNKLLPIINEILDNNQQSVDDFKNGKDRAVGFLVG
                     QIMKATKGQANPGVVNKLLKDELAKR"
     gene            complement(29158..30630)
                     /gene="gatA"
                     /locus_tag="ACG842_000791"
     CDS             complement(29158..30630)
                     /gene="gatA"
                     /locus_tag="ACG842_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HFQ0247353.1"
                     /translation="MTELHNRSLEELHHMLVSKEITVQDLTKTTFERIKETEPEVEAF
                     ITLNEEKALEQAKALDEKGIDESNVLAGIPIGIKDNIVTKDILTTAASKILYNFEPIY
                     DATVMDKIYQADMIPVGKLNMDEFAMGGSTETSYFKKTKNAWDPTKVPGGSSGGSAAA
                     VAAGQIPVSLGSDTGGSIRQPAAFNGIVGMKPTYGRVSRFGLIAFASSLDQIGPMTRT
                     VKDNALALTAISGYDEKDGTSSGVSVPDFAEGLTGDIKGMKIALPKEYLGEGVAPGVK
                     EAVLKAAETFRSLGAVVEEVSLPHSKYGVAVYYIIASSEASSNLQRFDGIRYGYRSEN
                     VKSLDDVYVNSRSEGFGEEVKRRIMLGTFSLSAGYYDAYFKKAGQVRTLIKRDFEKVF
                     ADYDLIIGPSSPTVAFGIGENINDPITMYMSDILTIPVNLAGLPGMSVPAGLSEGLPV
                     GLQIIGNYFDEKTMYQAAYAFEQATEFHKQQPAILGGKDE"
     gene            complement(30630..30935)
                     /gene="gatC"
                     /locus_tag="ACG842_000792"
     CDS             complement(30630..30935)
                     /gene="gatC"
                     /locus_tag="ACG842_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA];
                     GO_process: GO:0006450 - regulation of translational
                     fidelity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HFQ0247354.1"
                     /translation="MAISEEQVKHVAKLAKLAFADEELASFTDQLGKIIDMVEMLEEV
                     DTEGVPFTSNVVETVNVMRQDRAQAGWNRDELLKNVPETEDGFIKVPAIIDNGEAGA"
     gene            complement(31041..33077)
                     /gene="ligA"
                     /locus_tag="ACG842_000793"
     CDS             complement(31041..33077)
                     /gene="ligA"
                     /locus_tag="ACG842_000793"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HFQ0247355.1"
                     /translation="MEHEMTIDEAAKRAEELRTRLNQWSREYYVEDKPTVEDYVYDKE
                     YAELVAIEEQYPDLITSDSPTQRVGGKVLEGFEKVTHDIPLYSLNDVFSKEELIAFDQ
                     RVQKAVGRVVDYCCELKIDGLSVSLRYEDGNFVRGATRGDGTVGENITENLKTVRSVP
                     IKLKEPMNIEVRGECFMPKRSFVQLNQDREAEGKDIFANPRNAAAGSLRQLDSKITAK
                     RNLDTFLYTVADFGPMQAKTQYDALEELEKIGFHTNREKRLCHSIDEVWSYIEEYHDK
                     RVDLPYEIDGIVIKVNEFSLQDQLGFTIKAPRWAAAYKFPPEEVETLIENIEWTVGRT
                     GVVTPTAIMTPVRVAGTTVSRASLHNGDYIKLKDIRLKDTVLIYKAGDIIPEVSQVVL
                     DKRPKDSEEYQLPTHCPVCGSELVHLDEEVALRCINPKCPAQMKEGLNHFVSRNAMNI
                     DGLGPRVLEQMYDKKLVADVADLYKLTEEELLTLDKIKEKSANNILTAIDNSKDNSVE
                     RLIFGLGIRHVGAKAAKILAEHFGDLETLSKSDYESIIALDTIGDIIADSVVTYFSNE
                     EVHELMNELKQAGVNFEYKGLRNAQLQEVESPFKEKTVVLTGKLTRFTREEAKETIEN
                     LGGKVTGSVSKKTDIVVAGEDAGSKLTKAQELGIEVWTEDQMADALAKSRSVEE"
     gene            complement(33094..35328)
                     /gene="pcrA"
                     /locus_tag="ACG842_000794"
     CDS             complement(33094..35328)
                     /gene="pcrA"
                     /locus_tag="ACG842_000794"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006268 - DNA unwinding involved in DNA
                     replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HFQ0247356.1"
                     /translation="MSSKAELLNGMNPRQKEAVLHTDGPLLLMAGAGSGKTRVLTHRI
                     AYLIEEKEVNPWNILAITFTNKAAKEMKERVNAILASGGEDVWVSTFHSMCVRILRRD
                     VDFIGYNRNFTIIDSSEQLTLMKRILKELNIDPKKYDPRSILGTISQAKNSLQTPQDF
                     AKMQGSYYEEIAAKCYAAYQKELQYNQCMDFDDLIMNTIRLFEEHPDSLTYYQNKFHY
                     IHVDEYQDTNHAQYTLVNLLAGRFRNLCVVGDADQSIYGWRGADMQNILDFEKDYPDA
                     AVILLEQNYRSTKNILSAANQVIENNSNRKPKNLWTENKEGNKITYYRADNERDETRF
                     IVDRMQEEIRSNHRNYGDFAILYRTNAQSRVMEETLLKANIPYKMVGGHKFYDRKEIK
                     DILAYLNVLVNPQDSISFERIVNSPKRGIGPGSIEKLRSFASLHEWPLLEAAQNVDLA
                     NIGGKAGQQLGAFGEMIQEVTQMIPYLTVTELTKEVLDRSGYLEDLKIQNTLEAQARI
                     ENLEEFLTVTQEFDKQFEQQNEEDADAPEEKLTVFLNDLALVSDIDNLEEDASQVTLM
                     TLHAAKGLEFPVVFLIGLEEGVFPLSRALMEESELEEERRLAYVGITRAEEALYLTNA
                     FSRTLYGRTQYNRPSRFVEEIDQELLEIEGMRPTPKKTPVFAKKTAYSYKQPETAVVP
                     SKSATGGENNSWKPGDKVKHKKWGLGTVVRVSGTSKDLELDVAFPSQGVKRLLAAFAP
                     IEKA"
     gene            35483..37042
                     /locus_tag="ACG842_000795"
     CDS             35483..37042
                     /locus_tag="ACG842_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClC family H(+)/Cl(-) exchange transporter"
                     /protein_id="HFQ0247357.1"
                     /translation="MEKKGKIKKLDKTQIYFILKGIFVGLLTGIVVSLFRLSIEKLSD
                     LVRSIYEMSREQPIYLIGLAICCILAAFFVGYLVKNEPDIKGSGIPQVVGQLRGELSM
                     NWFSVLWKKFIGGVLSVGAGLFLGREGPSIQLGASIGQGAGQLFRSPSSEEKILISSG
                     ASSGLAAAFNAPIAGLLFVLEEIHHSFSPLVWLTSFASAITANFVSLYFFGLRPVLYL
                     GDLPSLPLKYYGSLVILGVLLGILGFIYQNVLLALPKFYNRLPLPAYFHGFVPFLLIL
                     PIGYLWPSTLGGGNQIVLAFGHASLPIIMIIGLFVLRFVFSMISYGGGLPGGIFLPIL
                     TLGALIGSFYGNIMVDFGMDPIYIKDFVVIAMAGYFTAIGKAPLTAIILVTEMVGSLN
                     HLMPLGLAALVAYIVNDLLGGNPIYESLLERLLGNTQTASLKGARTTFDFPVTAESSL
                     DGLMVRDFNWPKNMLLISIRRGNQEILTHGDTIMNVGDILVILTDEANLPRIKQEIEA
                     KSSITNLKQSF"
BASE COUNT        10242 a         8054 c         6688 g        12099 t
ORIGIN      
        1 agaatctttt gctgacgtct aaaaatagaa aaagaatttt cgtcatctat ttgataacgc
       61 atttgttttt gcagttctgc tgcagtcaga tggatcttcc catcgtttgt atcgatcatt
      121 aaaccttctg gaaacagttc aaccactgct cgatcccaag attcaagaga aagctcgata
      181 taatctttta ctggtaattc caattctacc ttaaaaaagt gcaaaacagc cgacagccct
      241 tctgcttgat agatttctcc taatgttagc ccctctagat ggaactctgg tgaaaaatgc
      301 agcaatgttt tattttcctg caattgaaca tatagaaatg tagatcgctc aatcagagta
      361 tcttttaaaa caactaaaaa attcattttt ctcccccttt tttcatcatt tttaaaatag
      421 catattttga aaagaaagtc tgatgcttag agtagaattt cataaaaatt tactatttca
      481 taagtattca cgagaaaaat acaaacaaaa aagcggaaat aacgctcatc cgccttcttg
      541 tttgtgttta tttcctcttc aaacgcttgt ctatgattca gacttttctc tatcgttatt
      601 ttcatctaaa agttcttgca ttccgataat ttctcgatat tgatgaatct tagtgatacc
      661 aatgactttg tctacatcaa tactaaaaca aataccatta cctggaatat cgatgttgat
      721 gccttgacca atcgcatcca ttactttatt cgtcaaacta tcgggtacta atgtcaacac
      781 aatatctttt tctggcgaaa tgtccaaacc taaaaatttc cctgaatcat gtacaccaga
      841 acctcttcca tgtaagatcg ttccgcctgc tgcacctgct tcttttgaaa agtcgataac
      901 atcttcgccc attcctccgt caacgatcgt cacaatcatg tgaagattca atgctacaag
      961 atctttcatt tgctttcccc caatttttgc aatctttctt tttcgattct gtctttttca
     1021 tcttttcgtt tataaatata tcctagtact aaagtggata atattggtgt caatgcaatc
     1081 attgcaacca caccaaaccc gtcaatgatc ggatctcttc cttcaatttg tgcagcgatt
     1141 gccacgctca ttgataaaat aaatgtcgaa cacatcgggc ccgttgctac tccaccgtta
     1201 tcaaaagcca ttgctaaaaa gatcttgtcc acttttcgtc ctaaaataaa tgctagcccg
     1261 tatcctggga tcaagaaata ataaagagag aatccgacga gcaaacgcca catagacaat
     1321 cctactgctg cgccgatccc aattgaaatt accatcagca tgattttcga tcggattctc
     1381 ccctcgctca attcttcaac ctgtttgatc attacatgaa tcgccggctc agcaaaccct
     1441 actaagaacc ccattaaaaa gccaagggga accagtatat aattgtaagg caatcccgca
     1501 atagcactgc ctaaatgctg tcctaccggt acataagcca tattgactcc gtgcaggaaa
     1561 aggatcaacc caatagtcgt tatcaagaaa cctttcataa tgtcaaagaa acgctttttg
     1621 cgcatacgaa aactaatgat attcattaaa acaaatagga ttacgatagg tgccactgcc
     1681 aaaatgactt ccatggtaac gatttcgatt ccatctatca cttgctgcca gaaactcatt
     1741 ggaagatcac ccccaaaatc atcacggcaa gaatcggccc taacgaagca ataccgacca
     1801 ttccaaaact gtcattttca tttttctttt gtttgatcat actagtcatc cctccggcaa
     1861 gggccaagat aaatggaaca gttactggac cagtagtcac tccccccgaa tcaaaagcaa
     1921 tcggcataaa ttcggaactg gtgaaaaatg aggcgatcaa aacgcctatg tagcctatag
     1981 ccatcaattg ataataggat agcttgaata cgacccgaag cagcgcaaag gctagaaata
     2041 ctccagtgcc gacgctgacg atcccaatca gcaacactct gccaatctct ccgtcagata
     2101 tatcatatac ttgttgtccc aaaacttgca cggaaggttc agcaatcgtg atcacgatcc
     2161 cgatagcaaa tcccaaactg accaaaacgg tcaaactttt ctttttgatc atatatttcc
     2221 ctactagatc tccgacttcc atcatagaat aatctgctcc aaacaaaaag agcgtcattc
     2281 cgatcatcat gattgccgca ccaaccagaa atgaagcgag gtcttctcct tctaacggtg
     2341 caaaaacaaa cgttaaaatg acaatcagga cagtcatcgg caatatagca gcaatcactt
     2401 ctttaaaatt ttttagcatg tatcctcctc ctttactctt taagtttacc ttaaaatgaa
     2461 agagttttcg agcacaaact taaaacgtta tcatttaatt tttctcaaca taaaataaca
     2521 cacgtcccaa aagtatcttt ttacttggaa aaatgacttt gaaggaacgc gcttattgtg
     2581 tttttttatt ttacagagcc gatcattcct tgaacgaatt gcttttgcaa aacaaaaaat
     2641 acgatggcag ttggtaaagt agcgatgact aatccagcca tgatcattcc atagtctggt
     2701 gtataggaag cacccatagc agataaaaca agcggaaccg tccgtttatc aggagattgc
     2761 aaagctacta agggccataa ataattgttc cagctactca tgaatgtgat gattgcagcc
     2821 gcggcatagg tatttttagc cgtcggcata tacactctaa aaaaaatact tagttctttt
     2881 agaccgtcta atcgagcggc ttctactaga tcttttggaa aagatttaac attttgtcga
     2941 aagaagaaga tcaaaaaagc agtgctaaca gatggcagga tgacaaccaa gaaactgttg
     3001 attcccaaaa ccgttccgtt caattgtgaa aacattcgat ataaagggat catcaatgcc
     3061 gcaaaaggaa ccatcatcga tagcagtaga atattgaaaa ttaggtcttt tcgtttactt
     3121 tgataaatct caaatccata tcctgccatt gacgaaatca gcaatgcaaa cacagtggtc
     3181 accaaagcga tcatcgctga attccaaagg gaacgagtaa aattcagctc attggaaaac
     3241 aaattttgaa aattgatgat caagttactg ccaatcttta tttttcccga tgtgatatcc
     3301 acgggtgtat tcgtcattcc caaaaccatc cagacaaaag ggaaaatgga aataaatgat
     3361 aggatgctta atgctgtata ctttaatact gacccaattg tcgaacgaag ttctaatgct
     3421 tgttttttga cgttggggcg ttcgattgtt tctttttcta ccatattttt catcgacttt
     3481 ctcctcctac tttcgtttgg attatcgaca atatcacaat aaaggaaaca ataacaaagg
     3541 aaactgctgc cgcatatcca aaattaggcg tgaatttata actcaaatta tagatgtatt
     3601 gagatagggt ggtcgttgca tttgctggtc ccccgccagt aatattttgt acttcatcaa
     3661 aaagctgcaa tgtcccgatc gtcgaagtaa tggaagtaaa caaaatgatt ggttttaaat
     3721 ttgggatcgt cactttgata aactgctgcc atttagaagc accgtcaata gatgctgctt
     3781 cataaatctc cgaatcaata ttttgcatgc ctgaaaggaa aaaaatcata ttgtatccag
     3841 tccaacgcca agtgatcgaa agaatgatca gaactctcgc ccaaattgga tgagtcaacc
     3901 agccaatcgg tgtgtcaatc ataccaatgc ccaacaacaa gttgtttaca aggccagatt
     3961 gagaaaatag acttttcatg atcagagaat aactgaccat cgaagtgata catggaagaa
     4021 agatagcggt tcgaaacaac cctttatatt tcaacttttt atcatttaaa aggttggaaa
     4081 taaaaagtgc tagaaacaac atgatcggta cttgtacaag taaatacagg aatgtattaa
     4141 aaaaagcttt tttaaaagtt gcatctgtca acatccgctg ataattacct aacccgttaa
     4201 atgtcatatt gacaggggga ccactttgaa aagacgtgat caaagccaaa accatcggaa
     4261 taaaaaccat gattgatatc aaaatgactg gcaagattaa aaaagcatgc ccgtttttga
     4321 ataaatcttt ctgcttcatt cttttccctc caattctatc attgattatc tacatgaaaa
     4381 gacaagaaag acaatatctg taaggcagac gtgatctttt ttgtcttttc gtttgtctga
     4441 tgaaaaagcg aagaaattca cttttttacc accgatcaat tagccaattg tgcttctact
     4501 tgtttttgtg tatctgctag taccttgtct gtctcttctc cattgatgat tcgatgaaga
     4561 gcttcagcta cgactgcttc aattgcatag gtctcatgtc cgtaattcac ttctggtatt
     4621 tcagatgtcc atgttgaaaa atcgtcaaag atcttttgac cactgtaaaa ttcagaagct
     4681 tcttgataag cttcttgttc attcgcgcta agcatcgtag atactagtcc gatttctttc
     4741 gcaagagtgc ccatcagttc ttcattcgaa gcaaacgttg cttctaggaa atctttcgct
     4801 gaatcttctc cagctactcc tttgatcaca taccagccag cgccgccaag gttagaagct
     4861 tgcgcttttt gcagattttc tggaagtgca ggtattggtg caatcttcca ttttccagat
     4921 tggttttctg ctccttggat cgttgaagaa taccaagcgc cagttgggct agaagctact
     4981 gctcctgatt gtatcgcagt tactccagca ttccagtcag aggtttgttc gactagatct
     5041 tctttcaaaa gagtggcaaa caattcaaga ccatacttta aagatgcatt gtcttggatc
     5101 gtgactgtct ccccatcttc agatgtatac cattctcctg cctgttgcat aatcatcctt
     5161 actcgaccaa ggtcagaagg attgacttca gcgattttct ttcccgtctt ttctttgaca
     5221 tctcgcgcta cttgaatata ctcgtcccac ttcagattct ccatatcctc ttctgtataa
     5281 cctgcttctg ccatcagatc cgtacgatag aagtttgctg ttacgcccga atcgaaagga
     5341 acaccgtata ttttatctcc tactttgttt accgcaaatt tatacggtgc aaaatgatct
     5401 tcctttacaa tagcagacaa attagcgaat gcatctggat aagaagtcaa atagccttgt
     5461 atcctataat cttcaatcaa gacgatattg ggcaatcctt ctgtattccc gctcgctaaa
     5521 gcggtgttca gtttctgaac gatatcatcc tgagacatcg taacaacttc tatctctgtt
     5581 tcttcgttct cataaacttt ttttgcttca ttcactgctt tgatattgaa tgtctcatcc
     5641 cacgcccaga cagtgacttt ttcttctttg tctgctgtac tactagcact tccgcatcct
     5701 gttaagaaac ctgatgctgc cgctaaacaa acgactccag caattaactt tctgatgttc
     5761 atttttatcc gctccttttc catccattag ttgtttacgt ttgctacatt catcatacca
     5821 aaatttgaaa acggtttctt tattattcag atacttcaaa aatttctcct aaaataataa
     5881 aaaaatacaa atcaactatg acaaaaggaa taatcttagc atctagacca aagatcgaaa
     5941 tggtgaggtt tctttaaaat aaaaatatcc ttgcattttc tcctagaaat aagtattttt
     6001 aaattacaag actattctta aggcgggtaa aaaaatgcca atacgcgtaa ttcttatcga
     6061 tgatgaacag acgattttag atggaatgaa acagttgatc aattggtctg agcacgggtt
     6121 tcaattaata gggacttttt ccagtccgct gaaagcgctt gatttttctt tggtacatgc
     6181 aatcgatatc gtggtcacag atgtaatgat gcctgaatta aatggaatcg aattatcgcg
     6241 gctattaaaa cagcaacaac ctgaaacaca gatccttatc ctctctagct atgatgaatt
     6301 tgatatggtt aaagattctt ttaaagaagg cgtcgctgat tatcttttaa agcctcgatt
     6361 gagtcctgat ttttttctaa attccttaac tgccgtttca caaaaaatca aaaaaaagcc
     6421 caacaaaagc ccaatcacca atcgttatga gcagatctgc gagaatcttt cacagcagat
     6481 tgctggacaa actcgtccat tgcttgaccc tcttcttttt gaacacacct tgtttttctg
     6541 ttatataccg aaaaacggcc attcagttca aaacaaggct ctaaacaaaa acagtcgatc
     6601 acccattcat tgatggatag cgacgattcg tttactattt atcctgtttc taccttttcg
     6661 gaagaatccg gctatgtaat caatactgat tccaaagata cacttaggaa gtttgttcaa
     6721 aagtttcgtc aagaagtttt tccaggtgat ttttttgttg tttctgattc ttttgcaatc
     6781 caagatatca atgaaatatt ccaagagatc agaaattgca ccaaaggaca acgtttctat
     6841 cataagggaa agaaccttgc ttatcagcat gaacttcttc cgttattaac caaccacgag
     6901 cagcaagcaa gtagttattt aacaaaaatc gttcataaag actacgcttt agctgtcaaa
     6961 gaacttgaga atttcttcac gacgatattg gaacagccta ttgatccagc ttatttgaaa
     7021 catgaggcaa tcaatcgttt ctatgcatta ctcaacgctt tggctgacga gcatgttggg
     7081 aatgaagaac tttctttgct caagatcgcc cttccttctt tattagccga tgctgagtat
     7141 ttcgaagatt tttctattct tgtcaaagaa acacttgaga aactcaccca cttttctgca
     7201 aatgcaaaaa aagacaattt cgatatcttg actttgatct acgagttcgt ccaggaaaat
     7261 tatgagaaag aaatcaattt acaactttta tccgaaaaat atcatttttc atatagctat
     7321 ctctcagcca tctttactga aaaatttggt atcaactttt caaaatatct aaaaaaagtc
     7381 cgtatcagta aagccaagct ctttttgacg gaaacacggc ttactttgac ggaaatctgt
     7441 gagaaagtcg gctataccga actgggttat ttctccagag tttttaaaga agagacagga
     7501 atgacacctt ctcaataccg taaaggaact tttgtaaaat gagaaaaaaa atcaaagagc
     7561 atgaactctt tgccaaactt ctgttcattt tgttcatcgg tctttttctg caagccatcg
     7621 tcatttcttt gtttatttat catcgatcaa gggatgccta tatccagctg ttcaatcaat
     7681 cgaatgatgt ggtactaaaa aagattcaat ctgaatttga aagtttgaat gatacaatcg
     7741 agaatacatt agccgctttt gattctaatc cagctgtcaa aagctatttt tccaatgatc
     7801 cagcccaaca tatggaacag cttcagcaac taagaacaat ccaaaaaatg aatgactctc
     7861 tttcaaaaat ccatccaatg atcgattacg atgtattgat ctttggagaa aatggaagaa
     7921 cttttgtcgg aaacgacatg ctcactgccg tatctgccga ttcttttttt caatctgcga
     7981 ttgctcagag agtgaatgaa cgtgctgctg atacacaaat gctttttgca caccacggat
     8041 tgactcttcg cgataagaaa gcacccagcg tcttctttgt caaaaaactg aaaaatacat
     8101 tgaatcacgt ttacggatat gctgttttat cgatctcatc caagcaacta gcgaatcttt
     8161 ttcaatctgt agtaaatcct gagatatcga agatctcctt catgaacgaa gaacagacga
     8221 tcatcgcatc aaatgaagaa aatacaattg ggagccgatc aatgattttc aaaaaactaa
     8281 cagtaggtga aacaacgatt tcaaatggac agcagttgac ccgtcttcct ctgtaccgcc
     8341 aggattgtac attactcagt caagtcgatc ttcattcttt atctaaccaa atgggcgtga
     8401 tttttccgat tattttgtat aatatcgtga cacttgcttt tgctggctca ttggtattta
     8461 tgtatttgga tcgacacaca aaatcgatct accgcttgat tgattcgcta aaacggctag
     8521 aggaagatgg cttaaaagct actgttcctg tacaaggaag ccatgaagtc caattgctag
     8581 ggaatacgat caatcaattg ctaagtaatg ctcagcatca ttatgaacag acgatccaag
     8641 acgaaaagaa gaaacgttcg cttgaaatcc aaactatgca agcacagata cagccccatt
     8701 ttatttataa tacgctgact agcttgaaat ttttgatttg gcagaaaaaa acagatgaag
     8761 cagttcaagg aatcgacagt ttcattactt tactaagaag tacgattggg aaaaaagaag
     8821 aagtgattcc agtcaaggaa gaaatcaaaa gcgtacaaag ttacatcgat cttttgagta
     8881 tgcgatatgg cgaaagaatc tcggctaaaa taatgattcc tgatgaactg cagtcattat
     8941 ctatgccgaa tatgatcctt caaccaatta tcgaaaacgc ctatcttcat gcttttcaca
     9001 ataaaacgca aggattcatc acggtgtacg gcaaaacaaa aaatcagctt cttttattcg
     9061 aagtgattga taacggcgat ggcttcgata ctgaaaatac caaacaaaaa aatgatcatt
     9121 tttcaggaat cggcatcagc catgtagatg agcggatacg cctaatgtat ggcgatgcat
     9181 atggcttaac gatccaatca gtgattggga caggaacgac tgtaacgatt tgtctaccgc
     9241 ttcttcctaa aaatcaatca gaatatcaaa aataaagaat agccaagcgc tgattttttt
     9301 attttaagtt aaataaatca atcagtattc ttgactttca tctacgcgtc tcttctactg
     9361 tcagcactgg ataacagccc aatatttgac tttctcctcc gagcatcttg atttcttgga
     9421 gcgcattctc tactaatgtc attggacggt ccaacaacaa atcgatcacg aaaaaatatt
     9481 ctcccaagct agttttcaat ggtcgggatt caatcttact taagttgatt tcccgccagc
     9541 caaaagctgc cagcatttta tgaagcatcc ctggccgatt agcaggtagt gtcaagatga
     9601 cactcatttt tactggagct gactgctggc tggtcatctt tcggtcaccg acgatccaaa
     9661 aacgtgtctg gttcaactca ttatcttgga tattctcagc taagatctcc aatccaacat
     9721 gctgtgctgt ttctaatgaa gcaatcgctg ctgcgtcttc tttcggatgt tctgctacat
     9781 acattgctgc agcagttgtt gattctgttg ctactaaagg aacattagga taatgcgttt
     9841 ctaaaaactg ttgtgattgt gctagtgctt gtggatgtga aaggattttt gtaatctttg
     9901 ttgctggatt gcctaaaagc tgctgcttga taggaagaac gatttcactt ttaacttcga
     9961 cttctggatg tttggaaaga agatcgattg tgtgatgaac agctccttcc aatgaattct
    10021 ctacaggaac gacagccaga tcgacttgtt tgcgaaacaa agcgcgcaaa caaacaggga
    10081 tggaagcata cgcagtcaat tgttctgtag gaaatagctg acaagccgcc tgataagtga
    10141 atgaactttc tggccctaga tagctgacgt tcatatgctt ttgatccttt ctataatggt
    10201 atcgatgatc ttttctggtg tttgattggt cgtgttgatt cgatgtgtcg cactttcttc
    10261 ataccaagaa agtcgttgtg ccaataatgc ttttatttct tctcttgttt ttccaagagc
    10321 taaaggtcga atagacgttt catcttgttg gatacgatca actagacagt ccgcttctgc
    10381 ctctaagtaa agaacaatcg gctgtgtttt taaaaaacgc cgattttctg cactttggac
    10441 aatccctcca ccagtcgcga taattttgtc ttcatttgac agctttctta aaatatctga
    10501 ctccactttt cgaaagcttt tttcaccgta atagtgaaaa tattcggcaa tcgacaattg
    10561 gatatgttcc tcaatctttg tatctaaatc tacatactct ttttgtaaac attgagctaa
    10621 ccctttaccg attgttgttt ttccagcgcc catgaaacca atcagcaaaa tgctagccat
    10681 gagcttctcc tcctgacaca agttctgcta aatcgtcaaa aaatgccgga taagagattg
    10741 aaacagcttc agctccttct agttcgcaag gtccatcagc tatcaaagca gctacttgca
    10801 gcatcatacc gattcgatga tcgcctctgc tggacacttt tgcgccatgc agaggtgtcg
    10861 gcccatggat gatcagaccg tctgctgtcg cttttatctt tgctcccatt ttctgtaatt
    10921 cttctgccac cgcatcaatt ctgttggttt cttttacttt taattcttct gcgtctttga
    10981 tgatcgtgat tccttctgct tgagtagcca ctaaagctag aatgggcaat tcatcaatca
    11041 gacgcggaat gatttctcca ccaatcgttg ctttcttcaa atgactcgtc ttgacactta
    11101 aatcagccga ttggttctgt tcattgtgat tcgtctgggt aatcgctgcg cccatctcct
    11161 ccaacacatc taagatacca gttcttgttg gattgacccc gacatttttt aacaacagtt
    11221 ggctttccgg aacaagaagt cctgctgcta gaaagaaagc agctgatgaa atatcacctg
    11281 gaacagttat ctgttgacca gttaattttt gcggtccggt cactatgatc gttttatctt
    11341 ccactgtgat ccttccgcca aattgacgaa tcatttcttc cgtatggtta cgggaacgtt
    11401 ctttttcaac gattacggat gtgccttctg cttgtaatgc cgcaaacagc aaagcagatt
    11461 tgacttgcgc actggctaca ggcatattgt atcggatagg tgataaagag cgggttcctg
    11521 tcacagtgat tggtagatac tggtcatttt cttctccttg caagtcagcg cccatctcgc
    11581 gtaaaggttc catcactcgc cccattggac gtttagacaa cgaagcatcc ccgaacagag
    11641 tagtcgtaaa aggctgtcca gccaaaatac ccatcaataa acgtatggtc gtaccggagt
    11701 ttcccatgtc aattgattcg gttggttgga caaatgagtg gctaccgcga ccatgaatct
    11761 tgatcacctc ttcctcttct tcgatctcga cacctaattg ttggaaggca tgtaaagtac
    11821 tcagacaatc ttctgcgcgc aaaaaatttt gtatagtcgt cgttccttca gcaattgctc
    11881 caaacatgat gctgcgatgg gaaattgatt tatctgctgg tatcctaaca gtccctctta
    11941 atccatgtat ttgttgtaat aattgcattt atccagcctc ctatttgatc cagcaatgat
    12001 aactggtatt agacataatg attctttttg cttgtaacaa atcctgttgg tttttaaaac
    12061 tgatttgcag tattcctaaa atatcttccc tggtctcttg gatcttcaaa ttaatcaaag
    12121 aaactcccgc ttttccgatc agtccagtta cttctgcaat aacgccaggt tcatctggca
    12181 catcgataaa taaatcaaaa aaggcaggaa tcgcgccttg cttatggaca ggcaactggt
    12241 ctcgactttc ttttgcccga ttgaaaaaag aaaagatttc cgattggttc tcagtcatga
    12301 tccattctgc tatttgtgtc atttcttgtt gccaatctgc caatcgttcc aataatatct
    12361 gcttattgct caacaaaata tctgtccaca tgacaggatc tgaggaagcg atcctagtga
    12421 tatctcgaaa gcctcctgct gccagttgtt tggctcgcgg atactcttga ttgaattgtt
    12481 ctgcctgatt gactaagcca gctgccagaa tatgtggaaa atgacttagc attcctgtta
    12541 tacggtcatg ttctcttgct gtaagagtta catattttgc tttggttccg caaaatagtt
    12601 cttgcagttt ttcaacatct tttttcgtct tttccaaagg tgtaaagatg taataggcat
    12661 tttcaaatag tttagcatct gctgcctgta tacctgattt atgagaacca gccattggat
    12721 gtccaccgac aaaccgaaga tttttctgct cagcggatgt cacgacttgc tgcttcgtac
    12781 tgctgacatc cgtaatgatg acgtgatcag acaagttgat tctttcaagg acatcaagat
    12841 aagctaaggt tgtacggacg ggaacggcta ataagataac gtctgcttgt tctgcacctg
    12901 tttcaaaatc acttgcaacg taatcaacga tctgctgttc tactgcttgc tcacgggttt
    12961 ttgactgact atcccaacca actactatca gttctggatt ggtctgacga atacaaagtg
    13021 ccatagaagc accaatcagt ccaagcccca cgataaaaac atgttgtgcc atcttatttg
    13081 cccccttttt agtaatgttt caagtagtca cggtactcct tcactgcagc tttcaactca
    13141 acaagcgaat cacttgggaa ttttgacaat agctctgtcg ctaccacagt ggctacaaca
    13201 ttttccgcaa cgacgctagc tgcaggaact gctgtactat ccgaacgttc tacacttgct
    13261 ttgtaaggtt cttttgtatc gatatcaaca ctttgaagtg gcttgtacaa tgtaggaatg
    13321 ggtttcatga ccccgcgaat gacaagtggt tcgccattgg tcattccgcc ttcgaagccg
    13381 cctagattat tggtacgtcg gaaatagcct ctttcttctt cccataggat ctcatccatc
    13441 acttctgagc ctggtaatga accagaagtg aatccgaccc caaattctac gcctttaaag
    13501 gcattgatac taaccacagc ttgtgcgatt ttggcatcta gcttcgtgtc ccattgtacg
    13561 tagcttccta atccagctgg aacgcctcca acgagcactt ctacaattcc tccgatcgta
    13621 tctccatttt tctttgtctg atcgatcaaa tcttttattc tttgctcaac tgctggatca
    13681 acaactcgta cttctgacgc ttcagaaatt tctttgattt cttgaatcga gatatttcca
    13741 ggtatttctg cttggattcc accaagacaa atcacgtgtc ctgctacgat gatgtcaagt
    13801 tcttccagaa ttttcttggc aattgcgccg attgctacac gcatggtcgt ctctcttgca
    13861 gaggaccgtt caagcacatt ccgaagatct cgatgatggt atttcatacc gcctaccaaa
    13921 tccgcgtgtc ctggacgcgg acgtgcaact cgccgcagtg attggtcttt ttcaggcact
    13981 tctgctacag acatgacagt tgtccaattt ttccaatctt tattttctac cactaaagtg
    14041 acaggtgaac caattgtttt gccgtgacgg atgccagacg tgatccgaac acgatctttt
    14101 tcgatcttca tccttcctcc tctgccgtaa ccagcttgtc tgcgagcgag ttcgtgattg
    14161 atttcttctt ctgacagagg taatcctgct ggtagtcctt caataatggc tgttaattct
    14221 ggtccatggg attctcccgc agtcatataa cgcatcttac agtcctccta agtaatcttt
    14281 gaatgtctct gtgggaacag gaacgatttt tgcttgccca atcttttcta gcaagatgat
    14341 attcaattgg cttcctcttg ctttcttatc atgggttatc gcgtggaata attcatctgg
    14401 atgacttaga tcgtacgtaa tcggcagatg gaatttttga agtatccttt ctaaagcagc
    14461 tgtcgatcca gttggagaca agccgatttt ttctgcatga gaagtgatgg ctaccattcc
    14521 aatagctact ccttctccat gactgactac cccatatcct gctgtatttt caattgcatg
    14581 gccaattgta tgaccaaaat ttaaaataag tcgatttcct tggtcaaaga catcttcttc
    14641 cactactttt cgtttgattt ccaacgtagc agtaatgatt ggaacagcat gttccagaag
    14701 atctttttcg tctttcagct cgttcagctg tttccaaagc gttgcatctg ctatagctgc
    14761 tgatttgatg atttcagcga ttccttctcg taaacgacgt accggcaaag tttttagaac
    14821 ctcaggatca atcaatacac cagctggctg gctaaatgtt ccgacaaggt ttttcgcttt
    14881 gtttgtattg actgctgtct tcccgccaat actagaatct acttgtgcaa gtagcgtagt
    14941 cggaatttga acgaaatgga tcccacgcat gaacgtcgag gccacgaatg ctgctagatc
    15001 acccaccatt cctccaccaa gtgcaagtat cccatcgctg cgggtaaaat cattatctgc
    15061 taaaaaatcg tataactcag ctgcttgttc taaagattta ctggtctcac ccgccggtac
    15121 gattgaaagg actggttcaa aattagcttg tctcaattgg aacatgattt tttctgcata
    15181 tagtggagca acgtttgtat cagtgatcac agctactttt tgaggtctcc aaatcgtttg
    15241 aagccatgat ccagcctgtt ctagtgcgcc tttttcaata tgaatatcgt atttttggtt
    15301 atcaacatgt acagtaagca ttcgtttcac ttctttcttt taaaaagctg gaattaatcg
    15361 agtgtctgaa tcttttccat tgcttctttc atgatcgcaa cttctttcat catgcgtgag
    15421 taagtctttt cattcaacga ttggggaccg tctgaccaag catttgcagg atcaggatgg
    15481 atctctacga tcatgccatc tgctccgctt gccacacccg cacgagccat tggtggtaca
    15541 agttcccaaa ttcctgtacc atgacttgga tcaacaatca cagggaaatg agttaatttt
    15601 ttcatcaatg ggacagcact taaatcaaac gtattacgtg tcgctgtttc gtaagtccga
    15661 atcccacgtt cgataaaaat gacaggagat ttgtcttcga ctgcaatgta ttctgcagca
    15721 ttaagccatt cattgatcgt accggaaatt ccacgtttca atccaacagg tttacctgtt
    15781 ttaccgacag cagatagcag tttaaaattt tgcatattac gtgcgccaat ctgtaggata
    15841 tcgctgtact cagccaccat atcgatatgc ccttcatcca tcacttcagt aatcactttc
    15901 attccaaatt catcagccgc ttgacggatg tatttcaatc cttcttcttc taaaccttgg
    15961 aaagcatatg gagaagtacg tggtttgaag gcaccacctc gcagaatttt tgcgcccccg
    16021 gctttggcca ttcgcgcaca ttctctgatt tggtctaatc cttcgataga acaaggacca
    16081 gccatcgtca cgaaactgcc gtcaccaatt tttactccgt ctacatccac gactgtattg
    16141 ccaggatgga attctcgaga agtcaatttg tatgtgttta aaatacgcac agcattttct
    16201 actccttcat agcgtgtaaa tgctccttct tgtaaacttc gcgtatcacc ttttagtcca
    16261 atcactactc gttctgttcc ttgatcgatt gagactttga atccttcttt ttcgatatga
    16321 tcgacgactt tttttatttg ttcttccgtt gcttcttttt tcataattac tatcattgtt
    16381 tttcccgctt tcttttgttt acttatctat cttggctgat tttctctact ttttcaaggt
    16441 ggttttacat taattcctac taaatgaccg tgtcctgttg ttctcatttt cgctccttct
    16501 ttcctactta tgacccagat aatcaacaaa aaaagatgtc cctgtaggaa attgcctcta
    16561 cagtgaacat cttcaacgag ccttaaaaag cgctgcaaaa atataaaata gccactatag
    16621 atgaatttct ctacgtggct gtaagtcgtg tttaaaaatc tttagccatc atagatacaa
    16681 acctggtttg tttgtaccca cgaagtcatg aatacaaacc tatctaaaat agctaaagta
    16741 atacgtattc agttttgtaa gttttctaga tttcataaaa aatcgctcca aactgatgta
    16801 agaattgtac ctagtataga cataaaaaaa tcgactgtca atcattttta tgaaactttt
    16861 tttaattttc tgatagttaa ctttgttttt cagaaaaata acaattggtt ttattgaaga
    16921 aaatagtcat gaacaaaagt atctgagaac ttttttactc tcttgttcat gacatataga
    16981 ataaagcagc tttgtcttgt aaactccgtt tttcaaataa taatctaaag attgctactg
    17041 aataataaag ccgatttcat ggtcctcttc gctgctgatt cggtaaggtg actcaacatc
    17101 cgctccccag ctattgatac ccccgactcc tcttactttt gcaaaaatcg taagaaccgt
    17161 tcgtctttct ggcggtagtt cttctatatg tgttgcctgt tccagttctt ctgctttgaa
    17221 tggtaaagcg gaaaaagcaa atggcgtatc ctgctgttca aatcgcaacg aaaaatcttc
    17281 ttgatcttta tccgtattat ccaacgtttg atttcttgtt agttctaccc atttcgtttc
    17341 catatgcatg ccgcattctt ggggaacaag gtatggggaa acggggagtc ctgccacttt
    17401 ataaatacct tcgacaccac cagccatccg atccggatat gtttcccctg ataggccttc
    17461 atactcaaaa aattttgctt tcgttggcat aatgaacctt aaacccaatg cgggtaattc
    17521 cggtaatcct ttttgtccat aataatgcat attgacaagc attttaccct ctccagcgat
    17581 ttggtaggaa attgtcactt gggtttttgg tactgtgttg atttccagtg taaaaatgat
    17641 ttcaaccgtt tcagcgtatt cattacttga atattgatta tttactggag caatcggcaa
    17701 atcgatcttt ttattgtcca ccagtacttc tatttctttg acttttggat acaatccggc
    17761 tcctaaccac ataccggatt taaatccaaa tccgctacct ctgtcattat cggtcgttgc
    17821 tcgccaaaaa actggaagtg tttcccggta tagccattct tttccgtttt tattgagcga
    17881 ttccagtccg cctcttgtat aagaaaaaat ataatgcgta ttccccgatt tgacacctaa
    17941 tgtacagtcg ccgaagacga ttgcaacttt ttccgtatcc atatgacttt tatttactgt
    18001 atttttcata ataatatctt acctcctgca tggcgggttt ttcttgtcca tctgcaaaaa
    18061 cgatcccatt ccctgagaat tcatagtcac ttggtttatc gtcaaagtca ccgccatagc
    18121 gcatcacttc tctgcctgtg attggatcag tcacttggat cgcctgatct ttaaaatccc
    18181 atatatagcc tccttggtac atttcataac gatcaagcaa cgtcatgtaa gaatccattc
    18241 cacctaacga attccccatg tcatgcatat attcacataa gatgaacggt ttttccgctt
    18301 tattttcaag gtagtcaatg atctcttctg gtggcgcata catgcagctt tttacatctg
    18361 aaatcgaatc ttcgaatgct ggatttggaa aattcccttc ataatggaca agtcgatcgg
    18421 ggtctacttt tttgaagtaa tcgttcattt gtcgaatgtt ttcttccgca taagattcat
    18481 ttccaagaga ccaaaaaaga atacttggat ggttttttaa tgtttcgaaa ttcgtttttg
    18541 ctcgatcaag aacagccgcc tgccactgag gtactgcacc tggtacgttg taagaaggct
    18601 caaatgcgcc cattttttgc catgagccat gggattcgag gttcgtttcc gccatcacat
    18661 ggattcctgc ttggtcgcat aaataatacc aagtgatctg gttggggtaa tgagatgtcc
    18721 gtacgccgtt gatctttgca ttttttatga tagccaaatc tttttccata tcttctatag
    18781 tgatggcgcg tccttttttc ggatgccact catgtcgatt taccccgttg ataatcatcc
    18841 gttttccatt gaagtagatg attttatctt tgatttcgat tttacgaaaa ccgaaggaat
    18901 agtcaatata ctccgttact cttttttctt cgtctatcgt ctccaattca agcttatata
    18961 aattgggctg aaaatgcccc cacagtgcca cattttcaaa tccatcgatg cgaatcgtca
    19021 tttgctcttt taacggatat tgctgttgat agatttcctt cccatcagga tctagtatcg
    19081 ctacattcag ctgactgttt ttggctactt gctcaaaaga caaatctact tcaagtttgc
    19141 cattttgaca tttgtcatct aagcgaggtt tgatccaaag atcttctaaa tggttttgtc
    19201 ctcgagcaac taagaagaca tctctgaaga tcccaaaaaa tcggaaaaag tcctgatctt
    19261 ccaagaatgc agctgtactt cttttgtaaa cctccacagc tagaatattt tcttcttcca
    19321 aataaggtgt taaatcaaat tcagacggag tgaacgaatc ttcggcatac ccgataaaat
    19381 gaccattcaa ccaaataaac attgcttgtt ctacaccagc aaaatgaatc gagatttgtt
    19441 ttccctttag accttcttct aatcgaaaag tcgtcaaata cgaaccaacc ggattgtctt
    19501 tagcagctga aaagatccct tccgcctctt tctcagggct tgtcgtgaaa gggggacggc
    19561 gaaatacatg tccctcccac ggatacatcg tattgatgta atggatcttg tcataatttg
    19621 ctaattcaat atgactagga acagtgattt catcaaaaga acaggcatca aaatcttttt
    19681 tataaaatgt ttttggtcgt accgaaggat ttggcgaata acaaaaacgc catgatccat
    19741 ttaaagactg tttaagggag gatgtggttt ctacttcttc ccttgttgca taacaaacat
    19801 gatcgctatg tgcatttgat tggttcactc taaaaacttg tggatcatct aaccatttta
    19861 tatttgcttt catacttttt tccttctctc tcataacttt ttgaaatgaa gcagttctgt
    19921 ttggaaatca ccttgaaaat cggggaagta aagtccaact gtcatcaatt cggcccccga
    19981 aaatatccgc ttcgaggtct gttcctgata aagtgcctca tcatctaacc ccatcagata
    20041 tacttcatgg aacgctggtt gagctgaagc tagtatcctc cctaaaaaca caatagcttc
    20101 atcttgttta ggagacacaa acatccatgc ggcaatattt tcttcaaaag gactcgccaa
    20161 acgataataa tggccatact ggatcaccgg acgaatcgtt tgataggata taatttggtc
    20221 ttttacttgc ttttcttctt caggagacag ctttgtcaaa tccagttcat aaccaaaaac
    20281 agcgctcata gctgtttctc ctcttgtttt caacgaagta gatctgtcgg tctgatgatt
    20341 tggtacagct gatacatgag ctgtgatgga cgaaatcgga taagcaaggc ttgttccata
    20401 ttggattttc atacgctcca ctgcatccgt gttatcactt gtccatgact gcggcatata
    20461 ataaataaac ccggcatcaa accgtccacc tccaccagaa cagccttccc ataagatttc
    20521 cggataatca gttgtcaaat gttcaagtag ttcataaagc ccgagcatat aacgatgcgc
    20581 tacttctcct tgtctctgtg ggtcaagggt aatcgaatag acatctgaaa gcgaacggtt
    20641 catatcccat ttgatgtagt caagaggaat cgtatctaaa atctttctca tttgtttctc
    20701 aatcgtctgc cgaacatcta accttgtaaa atccagtaca tgttgtgatc tggaagcaga
    20761 aggcattcta cctggctcct gcataagaaa atcaggatgt gttcgataca gttcagaatc
    20821 aaccgagatc atttcaggtt caaaccataa accaaatttc aatcctttgc tatgagcata
    20881 atctgctatt tcacgtaatc catttgtcaa cttgccttca tactcgaacc agtctcctaa
    20941 tgaagaattg tccgcatctc tttttccaaa ccagccatca tctaatacga acatttcgat
    21001 gcctagctcc tttgcttcat caatgatcgc cttgattttt tcactattga agtcaaagta
    21061 agtggcttcc cagttattga ccagaatcgg ccgttcagca aattgatgac gacctcttgc
    21121 gattctttca cgtagcaact catggtggat ctgactcatt ttgttcaatc cctgacttga
    21181 gtaagaaagg atcatttcag gtgtttgaaa agactggcct gcattcaatt cccacgaaaa
    21241 acgatggctg ttgatcccac cgacgactct caattgattg atctgatctt tttcaagttc
    21301 gaaagaatgg ttgccggaat aaacaaattg aagtccgatc gcttctccag tattttctgt
    21361 cgtatgatga tgaactaaag caataaagtt attcatatgg tgactgctcg ttcctcgacg
    21421 gctttcaaat acttttgttc cagataaaac agattgccgt gaaatctgtc tttcattgac
    21481 atgtgctcct ggcaaagcaa tgacttcatc aaatcgccta gtatgggcaa agtctagctg
    21541 gaaactagcc gctttttcta gtttgattgt ttctccggtt tcatttctta tctttacaga
    21601 tcgtgtgatc actgctctat cagtaaatat ggtatagaac aaatcgaaat aaatcgctgt
    21661 ttctctatcc ttaagtgaaa tcaccaacgt ttgtgcctct gacttgtcca tcacataagt
    21721 ttgaggcaag ccttcgatct ctggcttccc atctgtaata taatggctgt catatctcag
    21781 atccagtaag tctgatccat tcttgcgctg aatgatcgat gcgggcgttc gaaagtccat
    21841 gctgccatga gaagagtatt cttgcggtag tgtatctttc gacagagacc ggtcaggatt
    21901 taagggaaca tttcctgaaa atccacgatc tcttcgggga taatttttat tgtctttgta
    21961 ctgcttgact ggtttgccaa aataaatatg cgccaataca ttgctttctt ctatctttat
    22021 cagataagaa agaaatgtat ttcgtaaatg gaagactcgg ctcttttcat caaataagat
    22081 gaaattttta tctgtttttc tatgcgtaat atccatcatt tcactcccaa caaaacggaa
    22141 accgtttact tttttttata attcctgtat actgtttact aaacaatttt gtcaataaaa
    22201 attttttatt tttttgagga gtgtgtaaaa atggttggaa ttagagatgt cgcacgaaag
    22261 gcaaatgtct ctcctgctac agtttcccgt gtgttgaata acgatcagac gatccatgtg
    22321 gctcagacta ctcggcaacg gatagtagag gcagctgctg cgttggatta tgacatcagt
    22381 actcgtaaat atatcaaaaa gagaatgcct tcgattggtg tgatctctac gatcggcaaa
    22441 gcgagcgaag aacaggatat ctattacaaa catttgcgag tagggatgga agaagaagct
    22501 cgacgtctgc atttagggat gaatcgaatc tataatctat ctgataatcc taaacaatgg
    22561 gaagatctag atcagttagg tgcggtgatc gttgttggga ctgttacgaa agaatcgatt
    22621 accgccttgt taaaacaaaa catgcaaata gtcgtcgtgg ataaccctga catccagcaa
    22681 gaggtcgata tggtgtatgc agatcttgaa cggatgacta aaaccgtact ggaattgttc
    22741 ttagaaaatg gtcataaaag aatagcctat atcgggggat ataaagtcga tatggatgaa
    22801 ttcggtggga agcagatttc tataaatgaa aagaggatgc gtgcctatca gcattttatg
    22861 atcgatcatc acttagaaaa atatatgtct tatcatctag gtggctggac agaaaaacaa
    22921 ggtgaggtac ttgcgactaa tctattagaa aatgaatccc ctcttcctac tgccattctt
    22981 gtcgggagcg atccaatggc aattggtgtc tatcgggcat tgaaacaaaa aaatatccaa
    23041 attggagaag agattgttgt agcaagcttt gataatatcg agacaatcgc aaacctctcc
    23101 cctggcttga ccagtgttca aatcaatgcg aaatcaattg ggcaagcagc tgttcgaatg
    23161 gcattggagc gaattgacgg tatacgggac gaaccaatca ttttgactta tcctgcacga
    23221 ttgattgtta gggaaagttt tatcccaaga acaaaaaggt attgaaagag aggttgtgaa
    23281 aagccgcttc gtttcttcat actcaaaacg aaacggcttt ttcacaacct cttacttata
    23341 cttattcaca aaatccaatg tctgctggta acaattttgc gattctttta gcgtgtaatc
    23401 aaattggtat tcatgggtaa tctctttttt tgtatctgta taaaacaagg attgcacggg
    23461 tacctttaat tctgtcaacc gattttctaa tgcttgacct tgctcctgga atgagtatgc
    23521 atttccgtca gttatatacg ttggcggaaa ttccttagaa acatggtcag ccacactagc
    23581 ttctttcaat tgctcactcg ttttccaatc ttttgttcca atcaacgacc acgcaacggt
    23641 tttgacgaaa aacttcataa atcgatcatc cgaatgctgc tgtgctgtta gcttcaaatc
    23701 aacaggtccg caataagaaa tggttccttt cagactatct tttggaagta gctgtttgat
    23761 tttcatttgt ttagcatacg catcattcgt ttgtatagct gcatattgca atgcgatttg
    23821 tgaacctgca ctatctccac caaaaaataa ttgttccatg tctagcaatt tgtcgtcttt
    23881 ttcttcagct aataaatctt tgatcagttc attcacttga agaacttgat tagggtattc
    23941 tgaatcaggc gctaattcat aattcatcgc aaccactgcg atgttggcgt ctgaaactaa
    24001 ttttgtcgca aattctttga cacccgattt atctccgccg acgaatcctc caccatgtac
    24061 ccaaaataga acaggaacag gccccttgct gttttttgga tagtagatgt cataagtatt
    24121 ctctttatac ttggactcat acgtcaaatc tgtttttttc gccacgtttt tttctgcttg
    24181 atcgaacgtt tttttatcag tgatttgaac agtatttgaa aacatatggt tgatcaagaa
    24241 tgctccaggt ctgggggaaa gctggaaggc aagaaagatt gcaaacgcga tcccaagaat
    24301 gattccgaag aaccatccga taaatttaag aattttcttt ttcataaggc tccctcagta
    24361 gttagtatta agcattctga ttataccata gtgttatttc ctatcaagct ctcctagatg
    24421 atttaatgat tttccccaaa cttcttctgc aatgcttttg acaaaagaaa ttttttccat
    24481 tgttcttcaa acaaaacatt ttcttcttct gttgaagcaa atccacattg aggacttaag
    24541 cacaaacgat ccaaaggcag atatcgtgtc gcttcgtaga tccggtcaat cacttgctgg
    24601 tgctcttcca tttcgccatt tttcgttaca gtcaaaatct aagacaacta atttatcttc
    24661 agagactttc tctaaaagaa caaaactccc agcacgttca ctatcgtatt ctaaataata
    24721 ggcacggaca ttttcttgag taaaataatg ggtctgctac gtcgtcatcc cccctgatgc
    24781 cgccaaaatc gaataataat ttccgcgtca aacatgtata tttagtgttg aatcatttga
    24841 gaatatgaaa ataaaaatct ttttgatcaa tcagttaatc aaattgaaat aacgaaaaat
    24901 ctgagacaga atgacgttca ttctgaccca gatttgattt tgcacaactc aacagtcctt
    24961 attggcaaat cgccaaaata atgtagacta actaaaaaga ctaagaaaca ataacaaaag
    25021 ttaacacttc ttagtctttc tttagcacgt ctatttatta aagattttag ggccttttct
    25081 catgttttta cgatcattct taggtgtttc catttgtttg gatcggcctc caccttgttt
    25141 ctttttgatc aattctttca ttttttcttt ttggtccatt tttattctcc tttaacgaat
    25201 gaagcgacta attcgatatg atgggtttgc gggaaaagat ctacaggctg aactttcttc
    25261 acggcataac ctgcttctgc aaataactta gcatctcttg caaaagttgc cggattacat
    25321 gagatataga cgattcgttc tggtgacatt tcgattgctg attcaataaa atgatgatct
    25381 aatccttttc gtggagggtc aactacgatc acagatggtt tgatcccttt ttcttgccat
    25441 tttttcataa ctttttctgc ttttccgact tcataatgaa cattttcgat tttattattt
    25501 aaagcattga actgtgcatc ttcaattgct tctggaataa cttccatccc atacacctta
    25561 tccactctgt ctgctaaaga caagccaatc gtacctattc ctgaataagc gtcgatcaca
    25621 atgtcttttt tatctaactc ggcaaactcg atggctgttt ggtacagata ctctgcttgt
    25681 tgagtattta cttgataaaa tgactttgct gagattcgat aggtttttcc taatagctga
    25741 tcttcgatat agtctctgcc atataaaact ttttctttgt ctcctaaaat cacatttgtt
    25801 ttttcttcat tgatattttg gatgaccgaa accacttctg gcaattcttc atgaatagct
    25861 tcagcaattt ccttggattt aaagaatttt tctttacgag taactaaaac gaccatcatt
    25921 tcatgggaat aatgaccacg acggataatg atgtgacgaa gctgtccttc atttttttct
    25981 tcattatatc ctcgtacttg gaaccgttgc aagatatctc tgactacttt gattgcttga
    26041 tcgatgacag gatcttggat ataaaaatct tcgataggga ctagatcatg gctattcttt
    26101 ttgtaaaaac cggtggctaa ctgtccatcg atacggcgaa cagggatctg tgctttatta
    26161 cggtagccga atggttctgc cattccgacc gttggaagta caggaacttc tggcatctta
    26221 gcaatcttcg ccattacatg ctctacttgt ttttgtttga agacaagctg ttgttcatac
    26281 ttcaaatggg ctaaaggtgc gatacctatg cgaagtaatg cattgttttc tacaggctga
    26341 cgatcaggtg attgtgtaat aaatgtttcg acttttccat atccaaactt attgcctgct
    26401 tttactacta gtatttctac ttcttctcca gggagtgcat tttcgataaa taatggataa
    26461 ccatcgattt ttgctacccc cattccttca taactcaagt cgacgatttc gactgtatat
    26521 cgttcgtttt tctttactat ttgctgttct gtcatatttt gctcctttaa gcctattttt
    26581 caatcctact gaactctact ataaatcagc taattacaca actaaaaccg gcactgacct
    26641 tactacgttc agcaccggct tttgattaat tcttttgatt aggtgtttcg tctgctattt
    26701 ttccgtcacc gtcgatatct tcatccgtta aacgttcgac ttcttttacg aattctttgc
    26761 tgacaacctc taattcttct tcatctgtac ctgtgattgc ttcatcagga atctgatcgg
    26821 tattggcaaa gaactcaatg tgttgatgaa gattttggaa atgcatcggt gcttccccgc
    26881 cgtattcacc atctaagttg atcattactg gtcgattgat atcatcttct gtttcaacga
    26941 acaaactgct tgttttggta tagattattc ttggatcatc gacatgtcga ccgccattca
    27001 gcatgagtgc tgcaatatgc aagatctcaa agatatttgc tgtcttcaca atgattaaag
    27061 aaaacttccc atcatccaat ttggcatctg gcgcgatctg ttcgaatcct ccaacagagt
    27121 tcgtcagccc taggaaaaac atcgaagcat ttccgatata ttccccttca tcatacttca
    27181 agcgcatttt gattggcttt actcgaggga gcatctctgc tcctttcgca agataagcaa
    27241 gataaccaaa aatactcttt agttctgatg gaacttcata agtcaattct gtcaaatagc
    27301 cacctgcagc gatattgatg aagtaactgt tatcagaaag gccgatgtcc atttttacgg
    27361 tttggtgttt ttttatcact tctgctgctg ctttaatatt atctctagga atttttaatg
    27421 ctcgagcata gtcgttcgtt gtaccagctg ggatgatggc cattttagga cggtgttcaa
    27481 gcggagcgat tccattaacc acttcgttga ttgtgccatc gccaccagct gccactacta
    27541 gatcaaaacc tagttctgcg actctacatg cttcattctt ggctgaatct ggttctggtg
    27601 tagttgcata ggcgctggct tcaaatcctg cttcttctaa tgcctgtagg atatccgcca
    27661 aatttttttt caataattct tttcccgagg tggggttata aattacgcgt gcttttttca
    27721 ttttcagcct ctatcgtttc gctaattcat ctttaagtag cttattcacg acaccaggat
    27781 ttgcttgtcc tttagttgct ttcatgattt gaccgactag gaagccaacc gcacgatctt
    27841 ttccattttt gaaatcatcg acagattgtt ggttgttatc caaaatttca ttaatgattg
    27901 gcagtagttt gttcggatca gataattgca cccaaccatt tttttcaact acttcttggg
    27961 cttctccgcc atttgtgatc aattcacgga agactttttt ggcgattttt gagctgatcg
    28021 ttccatcggc gatcaaacga atcattcctg ctaagttctc aggtgtcaat tttgtctcag
    28081 ctaattccag tttctcactg ttcaagtaag cagaaacttc acccatcaac cagttagatg
    28141 cttgcttggc atctgcacca ttttccaaaa cagcttcaaa aaaatcagac atttcttttg
    28201 taagtgtcaa taccattgcg tcatactctg gaagtccaaa ctcacggata tagcgttcgc
    28261 gacgagaagc tggcatctca ggcaaactag cttttacttt ttgaatccat tcgtcatcga
    28321 tcacgaattt cgggatatca ggctctggga agtaacgata atcactagac ccttctttga
    28381 cacgcatcaa aactgtttta ttcgttgttt cgtcaaaacg gcgtgtttct tgttggattt
    28441 ctcctcctga aagaagaact tttgcttgac gtttttcttc aaaagctaaa ccttttttca
    28501 cgaaagataa agagttcaag tttttcaatt ctgctttcgt accgaactct tcttgtccat
    28561 aagggcgaag agaaatattg gcatcacagc gcatagagcc ttcttccatt ttcacatcgc
    28621 tgacttctgt aaattggata attgatcgca atgcttctag ataagcgtat gcttcttctg
    28681 gtgaacgcat atcagcttca gaaacgattt cgatcaaagg cgtgccttgt cggttcaaat
    28741 caacgtaaga ataaccgcca ataccatgca tgttttttcc agcatcttct tctaaatgga
    28801 cacgttcgat ccggattttt ttctttttac cttctacttc gatttcgatc catccgtcat
    28861 gaccgattgg ttgatcaaat tgggaaattt gatatgcttt tggattatct gggtaaaagt
    28921 aatttttccg gtcaaaatga gtatctttag agatttcaca gtttaatgcc aaagctgctt
    28981 tcatcccgaa ttccagcgca gctttattca ttactggcag cacgcctgga taaccccagt
    29041 cgatcacgtt tgtattgctg tttggttctg caccaaaatg agctggcgct ggagagaaaa
    29101 tttttgagtt ggtttttaac tcgacgtgga cttctagtcc aatgactgtt tcaaaattca
    29161 ttcgtctttc cctcctaaaa tagctggttg ttgtttatgg aattctgttg cttgttcaaa
    29221 cgcatatgcc gcttgataca tcgttttttc atcaaaataa ttaccgatga tctgcaatcc
    29281 aactggtaat ccttctgata aacctgccgg tacagacata cctggaagtc cagccaagtt
    29341 gacagggatc gttaagatgt cactcatgta cattgtgatt ggatcgttaa tgttctcacc
    29401 gattccgaat gcaactgttg gtgaagatgg cccgatgatc aagtcataat ctgcaaagac
    29461 tttttcaaaa tctcgtttga tcaatgtacg gacttgtcct gcttttttga agtaagcgtc
    29521 ataataaccc gcgcttaagg agaatgtacc taacatgata cggcgtttta cttcttcacc
    29581 gaatccttct gaacgtgagt tgacatatac atcatctaat gatttcacat tttcagagcg
    29641 ataaccgtag cggataccat cgaatcgttg caaattggaa cttgcttctg atgaggcaat
    29701 gatataataa acagccacac cgtattttga atgaggcaag ctgacttctt ccacaacagc
    29761 acctaatgaa cggaatgttt ctgctgcttt caatacagct tcttttacgc caggggccac
    29821 accttcgcct aagtattctt taggtaaagc aatcttcatt cctttgatgt cgcctgtcag
    29881 tccctctgca aaatcaggaa ctgaaacgcc tgaagaagta ccgtcttttt cgtcataacc
    29941 gctgatagct gtcaatgcta atgcattatc tttcaccgta cgggtcattg gtccgatttg
    30001 gtcaagagat gaagcaaagg caatcaaacc aaaacgtgag acacggccat aagtaggttt
    30061 catccctacg atgccgttaa aagctgctgg ttggcggata cttcccccag tatcactacc
    30121 taaagaaact ggaatttgtc cagctgcaac tgctgctgca gaaccaccag aagagccacc
    30181 aggtactttt gttgggtccc aagcattttt tgttttcttg aaataagaag tttctgtact
    30241 tccacccata gcaaattcat ccatgtttag cttaccaaca gggatcatat ctgcttgata
    30301 gattttatcc atgaccgttg catcataaat tggttcaaaa ttgtaaagga tcttagatgc
    30361 agcggttgtc aagatatctt ttgttacgat attatccttg ataccaattg gaataccagc
    30421 aaggacgttg ctttcatcaa tccctttttc atctaaagct tttgcctgtt ccaatgcttt
    30481 ttcttcgttc aatgtaatga atgcctcgac ctcaggctct gtttctttga ttcgttcaaa
    30541 tgttgttttt gtcaaatctt gtacagtgat ttctttcgat acaagcatat gatgcagttc
    30601 ttctagagaa cgattgtgta attctgtcat tatgcgccag cctccccatt atcgatgatt
    30661 gcgggtactt tgataaagcc gtcttctgtt tccggcacat ttttcaataa ttcatcgcgg
    30721 ttccatccag cttgcgcccg atcttgtcgc atcacgttta ctgtttcaac cacattcgac
    30781 gtaaatggaa caccttccgt atctacttct tccagcattt caaccatatc gatgatttta
    30841 cctaattggt cggtaaaact tgcaagttct tcatctgcga acgcaagttt tgctaatttg
    30901 gcaacgtgtt tcacttgttc ttcactaatc gccatactac cccttctttc taaacgagtt
    30961 aaaaactttg ctctctattt taacacaaga gtgccgtgtc tgaaattgtt tattgaaatt
    31021 tgtctgaaaa aactcatttt tcattcttct actgagcgac ttttagccaa tgcatctgcc
    31081 atttgatctt ctgtccaaac ttcgattcct aattcttgtg ctttggtcag cttgctgcca
    31141 gcatcttctc ctgctaccac gatatccgtt tttttcgata cgctaccagt cactttcccg
    31201 ccaagatttt cgatcgtctc ttttgcttct tcacgagtaa atcgtgttag tttaccagta
    31261 aggactactg ttttttcttt aaaaggagac tcgacttctt gcaattgggc atttcgaagc
    31321 cctttgtatt cgaaattgac gcctgcttgt tttaattcat tcatcaattc atggacttct
    31381 tcgttactga aataagtgac tacactgtcg gcaatgatat caccgatcgt atcaagcgca
    31441 ataatcgact catagtcact tttactcaat gtttctagat caccgaaatg ttctgctaaa
    31501 atttttgctg ctttcgctcc gacatgtcgg attcctaacc cgaaaatcaa ccgctcgacc
    31561 gaattatctt tgctgttgtc gattgctgtc aaaatattat ttgctgattt ttcttttatt
    31621 ttgtccaacg tcaataactc ttcttctgtc aatttgtaaa gatcagcaac atctgctact
    31681 agttttttat catacatctg ttctagtacg cgcggcccca acccatcgat attcatcgca
    31741 tttcttgaaa caaagtggtt caaaccttct ttcatttgag cagggcattt aggattgatg
    31801 caccgtaagg ctacttcttc atccaaatgg acaagttcac ttccacaaac tggacaatgt
    31861 gtaggcaatt ggtattcttc gctgtcttta ggtcgtttat ctaacacgac ttgcgaaact
    31921 tctgggatga tgtctcccgc tttataaatc agaactgtat ctttcaagcg gatatccttc
    31981 aacttaatat aatcaccgtt atgcaggctg gcacggctta ctgtcgttcc tgctactcgg
    32041 actggtgtca tgatagctgt tggcgttact acccctgttc gaccgactgt ccattcgatg
    32101 ttttcgatta gcgtttcaac ttcttctggc ggaaacttgt aagctgctgc ccatctcggt
    32161 gctttgatag taaaaccaag ttgatcttgt agcgagaatt cattcacttt gatgacgatt
    32221 ccatcgattt catagggcaa atccacacgt ttgtcatgat attcttcaat gtatgaccac
    32281 acttcatcaa tcgaatgaca tagacgtttt tcccgattcg tgtggaatcc aattttttct
    32341 agctcttcta gcgcatcata ctgggttttt gcttgcatag gaccgaaatc agctactgtg
    32401 tataaaaaag tgtccagatt tcttttcgct gtgattttag aatctaactg acgcaaagaa
    32461 ccagctgcgg cgttccgagg attcgcaaag atatcttttc cctctgcttc tctgtcttgg
    32521 ttcaattgga caaatgaccg ttttggcatg aaacattcgc ctcttacttc gatgttcatt
    32581 ggttctttta atttgattgg gaccgaccgg acagttttta ggttttcagt gatgttttct
    32641 cctacagttc catccccgcg agtcgcacct ctcacgaaat ttccatcttc ataacgcaaa
    32701 gaaacggaaa gtccatctat ttttagttca cagcaatagt caactacacg tcccactgct
    32761 ttttgtacac gttgatcaaa agctatcagc tcttctttgc tgaaaacgtc gttcaaactg
    32821 taaagcggaa tatcatgagt gactttttca aaaccttcta atacttttcc gcctactcgt
    32881 tgagtgggag aatcagacgt gatcaaatca ggatattgtt cctcgattgc tactaattct
    32941 gcgtattctt tatcataaac atagtcttcc actgttggtt tatcttctac gtagtattcg
    33001 cgagaccatt ggttcagtct tgtacgaagt tcttctgctc gtttagcagc ttcgtcaatc
    33061 gtcatttcat gttccatcat ttaccctcca ctactaagct ttttcaattg gcgcaaaggc
    33121 agccaaaaga cgttttactc cttgacttgg gaaagccaca tctagttcta gatccttcga
    33181 cgtcccgctt actcggacga ctgttcctag accccatttt ttgtgtttga ctttatctcc
    33241 aggtttccag ctgttatttt cccctccagt agcactttta gagggaacaa ccgctgtctc
    33301 tggctgttta tagctatatg ctgttttttt agcgaataca ggcgtttttt taggcgtagg
    33361 acgcatgcct tcgatttcca gtaactcctg atcgatttct tctacaaaac gagaaggtcg
    33421 gttatattgc gtccgtccat ataatgttct ggagaaagca tttgttaaat acaacgcttc
    33481 ttctgctcgc gtgatcccca catatgcgag acggcgttct tcctccagtt cgctttcttc
    33541 catcaatgct cgagataaag gaaaaacccc ctcttctaga ccaatcagga atacaactgg
    33601 aaattctaat cctttggctg catgcaaagt catcaatgtc acttgggaag catcttcttc
    33661 taaattgtca atgtcggaaa caagtgccag atcatttaag aagaccgtta gtttttcttc
    33721 tggtgcatca gcgtcttctt cattttgctg ttcgaattgt ttgtcgaatt cttgtgtgac
    33781 cgttaaaaat tcctccaagt tttcgatgcg tgcttgtgct tctaatgtat tttggatttt
    33841 taagtcttct agatatccgc tacgatccaa aacctctttc gtcaattctg tcactgtcaa
    33901 gtatgggatc atctgagtga cttcttggat catctctcca aatgcaccta attgctgtcc
    33961 tgcttttcca ccgatatttg ccagatccac attttgcgct gcttcaagca gtggccattc
    34021 atgaagagaa gcaaaactgc gtagtttttc gatagatcct ggtccgatgc ctcgtttggg
    34081 cgaattaacg atacgctcaa aactgattga atcttgagga tttaccagca cattcaaata
    34141 cgctaaaata tctttgattt ctttacgatc gtagaacttg tgccctccaa ccattttata
    34201 aggaatattt gctttcaaca gagtttcttc cattacacga gattgtgcgt tcgtccggta
    34261 caagatggca aagtcgccgt aattccgatg attgcttcga atctcttctt gcatccggtc
    34321 tacgataaaa cgggtttcgt ctcgttcatt atctgcacgg tagtaagtga tcttgtttcc
    34381 ttctttgttt tctgtccaca agttttttgg ttttcgattg ctattgtttt caatgacttg
    34441 gtttgctgcg ctaaggatgt ttttcgtgga gcgatagttt tgttctagca aaattactgc
    34501 agcatctgga taatcttttt caaaatccaa gatgttctgc atatctgcac cgcgccagcc
    34561 gtaaatactt tgatctgcat ctcccacgac gcacaggttc ctaaatctac ctgccaataa
    34621 attgactagc gtatactgtg cgtgatttgt atcttggtac tcatctacat gaatataatg
    34681 gaatttgttt tgataatacg tgagtgaatc aggatgttct tcaaataatc gaatcgtatt
    34741 catgatcagg tcgtcaaagt ccatacattg attatattga agctcttttt ggtaagcagc
    34801 ataacatttt gccgcaatct cttcgtaata actgccttgc atttttgcaa aatcctgagg
    34861 ggtctgcagg ctgttttttg cttgagagat tgttcctaat atacttcgag gatcatattt
    34921 tttcggatca atgttcagtt cttttagtat ccgtttcatc aaggtcagct gttcagaaga
    34981 atcgatgatt gtaaagttcc ggttataccc gataaaatca acatctcttc ttaaaatacg
    35041 cacacacatc gagtgaaaag tcgataccca gacatcttcg cctccagatg cgagaatcgc
    35101 attgacacgt tctttcattt cttttgcagc cttattggta aaagtaatcg ctaggatgtt
    35161 ccaaggattg acttcttttt cctcaatcaa ataggcaatg cggtgtgtca gcactctcgt
    35221 ctttccactt cctgcacctg ccattagcag taatggtccg tcagtgtgaa gaacagcttc
    35281 tttctgacgg gggttcatcc cgtttaataa ttcagctttg gatgacatta acaacatcct
    35341 ttcttttatc aatcttgtta attatagcat tttcttggct ttcattaaac tagctagttc
    35401 aaaatcattt gtttcttttt atggattttt ccttgtcaat tcgttgattt cgcgctaaat
    35461 taaaagaaag cgaggcgttt gaatggaaaa aaaaggaaaa atcaagaaat tagataagac
    35521 tcagatatat tttattctaa aaggaatatt tgttggactg ctgacaggaa tcgtcgttag
    35581 cctgtttcga ttatctatcg aaaagctaag cgatttagtt cgaagtatct atgaaatgag
    35641 tcgggaacag ccgatttact taattggcct agctatttgc tgcatccttg cagcattttt
    35701 cgtgggttat ctggtcaaaa atgaaccgga tatcaaggga agcgggattc cacaagtagt
    35761 gggacagctt cgcggggaat tatcaatgaa ctggttctct gttttatgga aaaagttcat
    35821 tggcggtgta ctttcagttg gtgcgggcct ttttctaggt agagaaggac cttctataca
    35881 gcttggcgca tccattggac aaggagctgg acaattgttt cggagtccat cttcagaaga
    35941 aaagatttta atttctagcg gtgcaagtag cggacttgca gcagcattca atgctcctat
    36001 cgcaggatta ctctttgttt tagaagaaat ccatcactcc ttttctccac ttgtttggct
    36061 gacatcattt gcttcagcaa tcactgctaa cttcgtctct ctttactttt tcggcttgag
    36121 acctgtttta tatctaggtg accttccttc tttgccgttg aaatattatg gttccttagt
    36181 gatcttagga gtacttctag gcatcttagg ttttatttat cagaatgtct tgctggcttt
    36241 gccaaaattt tacaaccgat tgcctttacc agcttatttt cacgggttcg ttcctttctt
    36301 gctgattttg ccaattggtt atttatggcc tagtactcta ggcggaggca atcaaatcgt
    36361 tttagctttt ggacatgctt ccttgccaat cattatgatt atcggtttgt ttgttcttcg
    36421 attcgttttt tccatgattt cttacggtgg gggtcttcca ggggggattt tccttccgat
    36481 attgacattg ggggctttaa tcggtagctt ttatggaaac attatggttg atttcgggat
    36541 ggatccaatc tacatcaagg attttgtcgt tattgccatg gctggttatt tcacagcgat
    36601 tggaaaagca ccgctgacag caattatttt agtgacagaa atggttggct ctcttaatca
    36661 tctgatgcct ttaggattgg ctgctcttgt tgcttatatc gtcaatgacc ttctcggcgg
    36721 taatccaatt tacgaatcat tattggaacg tctacttgga aatactcaga ccgcctcact
    36781 aaaaggtgca aggacaactt ttgattttcc agtaacggca gagagttctt tagacggatt
    36841 aatggtacga gacttcaatt ggcctaaaaa tatgctgcta atctctatac gcagaggaaa
    36901 tcaggaaatc ttgactcacg gtgatacgat catgaatgta ggagatattt tggttatctt
    36961 gactgatgaa gcaaacttgc caagaatcaa gcaggaaatc gaggctaaat cttctatcac
    37021 aaatttgaag caatcatttt aacgaacatg gaaaaactat tttacaacaa ctattcatct
    37081 aca
//
LOCUS       DAZKLM010000017        36777 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3764
            SAMEA115706913-rid22582563.denovo.017, whole genome shotgun
            sequence.
ACCESSION   DAZKLM010000017 DAZKLM010000000
VERSION     DAZKLM010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706913
            Sequence Read Archive: ERR13196647
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 36777)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36777)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287823.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287823.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,004
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,849
            CDSs (with protein)               :: 2,849
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 41 of 96
            Pseudo Genes (incomplete)         :: 52 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 15 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36777
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706913-rid22582563.denovo.017"
                     /isolate="VRE3764"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            156..3434
                     /locus_tag="ACG842_000796"
     CDS             156..3434
                     /locus_tag="ACG842_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HFQ0247358.1"
                     /translation="MLKKRIKYSALMMNLLMLSTPVLISVVEVAANEQQSVNEENAHV
                     NIEGMSTMEEIPRTSEDMDTEEGNDPMMKESENEKTKKQSEEANEEWKGVVFGESTSA
                     SRYAIEPLEDGVRLSSTNNGGKFQSSGSDGMTYYYQAIPKDINFRMRATIEIESWTYT
                     NAQEGFALMVRDAVPKNHLFGQAFYSNSFAILGSRIEYVWDSDRQEVVNTSGSKYTMR
                     LGLGTRAITGISSEDPQAAPAPGSVSVETTPLETSAGFLEKETGSYNIFGNGHGNLFP
                     ATNSITKLDVEMKKTNTGLEAYYYDPETGEEMASDIMYDWEKLYASDESVIYAGFAAA
                     RNMTIKVEGIEVAYSDPATDPPGQERPTRLIDPIYTVTSSNHSGNQDHTMSFRANADG
                     LLTIKNKATGEVLKNAKNLAITANREFDYQTKLMEGTNEFTFSFTPDKNYPPEEYVEM
                     TSYETKEILHTVDYRKPKYSTVYVTPEGSDAGNGSRQNPLSLTQALRYAYAGQTIVMA
                     PGTYSFERGLTIPKGVNGTRENPIQLIAEKNPENKRPVLDFKGTGNGMTLFSHYWGLR
                     GFDITNSAAMQKGLQISGNHNLIEEIHAYRNGNTGIQISGSSNDPFEAWPAHNLVKNC
                     TSFENADPGFEDADGFAAKLTIGEGNVFDGCIAYHNADDGWDLFAKNETGSIGKVIIK
                     NSVAYRNGWVPGIEGEGNGNGFKMGGSSLTGPHELINSLSFENLAKGIDSNSGTDIIV
                     NSSTSFNNGSYNVALYTSSAGNTDYHASGILSFRTENLEMREQIRPLNQNEDQIYHSL
                     NYYWNEREKQSENTLGHTISKDWIESLNTCSKEGQQPVTRYENGSIHVHGLMQIKPGN
                     RQTEKERVDGLPLSVGAIFDGETSPSSEYPEYAIVQEPNQTKPNEQNNPIIIQEANGV
                     AENAEGNIIELKPFTVFVSTFTPNQGTPVFEDMRYFPEKIQIVETQSSAQFSNHTAIF
                     SAPSKGEYTLEVTYRSEIFDGEKWSAHTESVTQQKAITVRETADVPPGNDKPERENPG
                     NESPNGKPNGKPSHPDRTAKESEKNGTQGLPKTGEFNNPLLALAGGILLIGVVVYVMK
                     QRKK"
     gene            complement(3975..5882)
                     /locus_tag="ACG842_000797"
     CDS             complement(3975..5882)
                     /locus_tag="ACG842_000797"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M13-type metalloendopeptidase"
                     /protein_id="HFQ0247359.1"
                     /translation="MNKERLKQDFYEAVNEEWQKTAKIPADKPATGGFQDLVDGIDQL
                     LMGETDDLLAHPEKVSSEKMKHFLAYYQLANDYDRRNELHAQPLQPIIEKVESLQSFD
                     ELNKQFPEWLLDTLPLPFNLDVDADMKNAQTNAFFAYPPSLFLPDKTYYASDHPNGAQ
                     LIGVFFDMAVQLLELAGKEKSQAEAITEQAVQFDKLIAPHVKSAEENADYSKMYNPQS
                     FDTFIKYTDKLDLKKLVTGLIGTTPDKVIVTDPVYFDHLNDILTEAHFPLFKSWMIIK
                     TVRSLSGYLSEEFRQVSGIYSRVLSGTDEAMPQKKAAYYLASGQFDQVVGDYYGKKYF
                     GEAAKKDVEQMVKKMIAVYKKRLEENDWLGDATKEKAVIKLNNLGIQVGYPDKIPALF
                     DQFKTVPADQGGTLLSNTLVFSKLTLKDRFSKWNKPVDRSEWEMSADTVNAYYHPFRN
                     VIVFPAAILQAPFYSLEQSSSANYGGIGAVIAHEISHAFDNNGALFDEYGNLNNWWTE
                     EDLTQFQNKAQAMIEEFDGLPFADGKVNGKLTVSENIADAGGLSCALEAAKTESDLSI
                     EEFFVNWATIWRTKAKKEYQQLLLQIDVHAPAKLRANVQPKNLDEFYETFGITEQDAM
                     YLAPEKRVRIW"
     gene            6012..6887
                     /locus_tag="ACG842_000798"
     CDS             6012..6887
                     /locus_tag="ACG842_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide dehydrogenase"
                     /protein_id="HFQ0247360.1"
                     /translation="MKKRRRSQLNQVVDKPLYFKVDKKIKKLASTQQLQSRKSKLLFL
                     ALVFEDQSYVIIDQSGHPVEYSPAKYTYQEGLSCKKWKLINEEPIELSRWINRKEDIP
                     VLIEEKRSGKELANCWVGLPEERFLRYKKWATPSGYLCGTYAAAVLLAYYQDYRKGWM
                     LPNEIRKKNTADSLVLTKALRSQIQPLGLPTIPFQVSTGISSFLKKNGNHERARATLL
                     GSWQRATKRIREGKPVMIGILKVLGSTYGNHWVTAYAYFETETGERYYKVHDNWGDYH
                     KVIPASWSNGTVSLP"
     gene            6967..7941
                     /locus_tag="ACG842_000799"
     CDS             6967..7941
                     /locus_tag="ACG842_000799"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HFQ0247361.1"
                     /translation="MSNKYQDDTLKIFSLNGNRPLAEKIAKVFGTELGKSVVKQFSDG
                     EISINIEESIRGDHVYIVQSTNAPVNDYYMELLIMIDAMKRASAKTINVVLPYYGYAR
                     QDRTAKPHEPITAKLIANLIEEAGATRVLTLDLHTVQVQGFFDIPVDNLFTMPLFAHY
                     YRQLGLTGDDIVVVSPKNSGVQRARSLSEYLNSTLAIVDHADDASTEGGYVIGDVQGK
                     TCIMVDDILNTGATLARAANVLKENGAKEVYACASHGLLSDHAKSILDNAPIKDICIT
                     DSVFTEENRHPENLTIVTCSELMGEAVKRIHENTPMSPLFRLEEKEFE"
     gene            8121..9266
                     /locus_tag="ACG842_000800"
     CDS             8121..9266
                     /locus_tag="ACG842_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HFQ0247362.1"
                     /translation="MNEVKTIYLDHAATTPMHPQVIEAMTAIMQDTFGNPSSIHGFGR
                     HAHEKLETARQIIADSLQAKPHEIIFNSGGTEGDNTAILETAFSRQKEGRHLITTAIE
                     HPAVLNTMKYLETKGFDVTYLPVDEKGNLSIEEVKKALREDTILVSVMYGNNEIGNLM
                     PIKEIGELLADHPAYFHTDAVQAYGNQTIHPEELGIDLLSISAHKINGPKGVGFLYKR
                     DGVNIPALLLGGEQEEKRRAGTENLAGIWGMAKAVELLSPEEREARNNKYKGFQQLIL
                     DTLEANEIPYQVNGDLTNKLPHVLNLRLIGEANDLLLMKLDLNGVAISTGSACTAGNI
                     EPSHVLEAMYGEDTPILKESIRISFGLGNTEDDIQYFLDQLVRAVKK"
     gene            9403..9747
                     /locus_tag="ACG842_000801"
     CDS             9403..9747
                     /locus_tag="ACG842_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1831 domain-containing protein"
                     /protein_id="HFQ0247363.1"
                     /translation="MAFETTATVLGAPISYRLHPDAKRYTLRDNGFTETNGGNYQLIR
                     PLDATPQSKEGFKLKITVSKDIQTLKMSITTANGLKAVNIFKDPKQKMSQDKFYFLMD
                     GMISRGLFEKVE"
     gene            10018..11304
                     /gene="efmA"
                     /locus_tag="ACG842_000802"
     CDS             10018..11304
                     /gene="efmA"
                     /locus_tag="ACG842_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EfmA"
                     /protein_id="HFQ0247364.1"
                     /translation="MENEQSVVLTNWKRNYLFFLSGQFLSGITSMVVQYAIIWYLTRE
                     TGSATILSFATLLGMIPMVLLSPFVGPLVDRWDKKALLIVTDIIVAIFALILAVVGTI
                     SESFPIWLVFVSLFMRSVAQTFQMPTIQSIMPTIVPSSHITRTNGQLGMVQSANFIIA
                     PALGAALFSVVPVNYLILLDVLGAVFGVGLLIFVKIPKVSPEILEVPLTIFKDAKFGL
                     QQLMDNKGLWYITINGAFVMLLFMPAISLYPLMTLDYFGGSVGQAGAVEVVYAVGMLL
                     GGALISFIGTWKDRMKPIIIAYIIMGLTIGASGLVPNDSQGFLYFLILNAGAGCATPY
                     FNTLLMAMIQQSYESNVLGRVLGNFNSLMNLAGPIGLLFAGPLADRLGVEKMFLFSGI
                     GILLCGIVLFLTSAARNYDKELQKKLVKEHHEQKDE"
     gene            complement(11442..12041)
                     /locus_tag="ACG842_000803"
     CDS             complement(11442..12041)
                     /locus_tag="ACG842_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFQ0247365.1"
                     /translation="MNYFISDMHFFHERLLGKNDFAPRLFANVDEMNQQLITSWNAVI
                     KETDHVYHLGDLAMHPQYEKANAEILELVTQLQGTIHLIKGNHDSRAFFRYLEKNDPR
                     LTDGSPKFYFYDVGAIVKFDHQQYYLTHYPMLLGPNDTIRNLHGHIHHYSVPIENDVN
                     VGVDAPERELLKEKLPFGTPLSEQQIKEIYEAKRKYDRS"
     gene            12445..13566
                     /gene="mnmA"
                     /locus_tag="ACG842_000804"
     CDS             12445..13566
                     /gene="mnmA"
                     /locus_tag="ACG842_000804"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HFQ0247366.1"
                     /translation="MTDNSKTRVVVGMSGGVDSSVTALLLKEQGYDVIGIFMKNWDDT
                     DENGVCTATEDYKDVAKVAAQIGIPYYSVNFEKEYWDRVFEYFLAEYRAGRTPNPDVM
                     CNKEIKFKAFLDYAMDLGADYVATGHYAQVTRDENGVVHMLRGVDNNKDQTYFLSQLS
                     QEQLSKTMFPLGGMEKSEVRAIAERAGLATAKKKDSTGICFIGEKNFKQFLSNYLPAK
                     KGNMVTLDGEVKGQHAGLMYYTIGQRQGLGIGGGGDSQEPWFVVGKDLATNTLYVGQG
                     FHHPALYATSLDASEIHFTTNEPMPKEFKCTAKFRYRQQDVPVTVRLLDDNRAEVVFD
                     EPVRAITPGQAVVFYDGMECLGGGLIDHAYQETKVLQYV"
     gene            13812..15233
                     /gene="arcD"
                     /locus_tag="ACG842_000805"
     CDS             13812..15233
                     /gene="arcD"
                     /locus_tag="ACG842_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0043858 - arginine:ornithine antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine-ornithine antiporter"
                     /protein_id="HFQ0247367.1"
                     /translation="MEKKSTGISKWGLVALVVSSSIGSGVFGITSDLASSAAPGPAIL
                     SWIIVGIGILALVLSLNHLGEKRPDLDGGIFGYAEASFGKLGGFISGWGYWLSAWLGN
                     VAFATMLMSAMGEFFPIFKGGQNVPSILFASIFIWGLTLLVNNGVEGASVINTIVTIC
                     KLVPLFLFLIFVFIAFKVNIFTADFWGNVSDNLVNGTGQQGTIWLQIKGCLMVMMWVF
                     VGIEGASVLANRAKKRSEAQQASIIGLLCLLFIYILASILPYGVLSQEELATISQPAM
                     ANILKGIVGNWGAALINIGLIISIIGSWLSWTMLPAETTMLMARDGLLPKGWGKTNKK
                     NAPTYSLVVTAILTNLFLLTFLVTDYAYQFAYSLCTAAILICYLLVGIYQVQFSLKEG
                     IKKQVVIGGIAVVFELMGIILAGFSYVLLCSIAYLPGFCFYWIACRETKHQITGKEKV
                     MIGLICVAALLSIGLLAVGWIKI"
     gene            15247..16365
                     /locus_tag="ACG842_000806"
     CDS             15247..16365
                     /locus_tag="ACG842_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HFQ0247368.1"
                     /translation="MKIANFGVEEWLNKWEKKAVYDIAQSSIEALTLDELVGLDGTSV
                     SKFFEKRKNEPLDYGWIEGSDVFKQEVASLYQTVDPENILQTNGATGANLLALYALVE
                     AGDHVVSMLPTYQQLYDIPKSLGAAVDFVHLKEEEDWQFDLEQLEALVKPETKIICLN
                     SANNPTGTLLDRKTLEKIAEIARTVDAYVLIDEVYAPLTDKGEFLSIVDVYEKGIATN
                     SLSKTYSIPGIRIGWTATGPELAEVFRKYRDYTMICGGVLSDDLAVHALKNKEKILAR
                     NKKIITENLVILKQWVANEPKVELVAPNYVSTSFIKLAIEEDDQTFCINLLEETGVLL
                     VPGSAFDLPKHARLGYCCKKETLEKGLFLLSEFLKKQA"
     gene            complement(16425..17717)
                     /locus_tag="ACG842_000807"
     CDS             complement(16425..17717)
                     /locus_tag="ACG842_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HFQ0247369.1"
                     /translation="MAARIKEKLFRKRETWEQNLFVLWFGTFMAGIGFSLVMPFMSLY
                     IDTLGNYSTSQLNFWSGLTFSSTFLVTTLISPWWGKLADQKGRKLMLLRASLGMAIVI
                     SLMGCVTSVYQLVALRLLQGIFSGYISNATALVATGTPKEKSGQVLGTLATGSVTGTL
                     LGPLLGGISASAFGYRPTFFITGIILFFVFLLSLFFVHEKFVPVKKENMVSAKQIFKD
                     LKYPHVVIGMFVTTMIIQASNNSISPIISLYIRQLLHGHGNVTLISGIIASIPGIATL
                     IAAPRFGRLGDKIGSERILAIGLGFAILVYIPMAFVTNVWQLAALRFLIGISDACLLP
                     AVQALITKYSPHQAAGRIFSYNQSFQATGNVVGPMIGSSVSSAFGYRGVFLSTSLLVL
                     VNFLLVRHNTQEIHQQETAEEKASGVSIASGHSPSHRQ"
     gene            17867..18427
                     /locus_tag="ACG842_000808"
     CDS             17867..18427
                     /locus_tag="ACG842_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFQ0247370.1"
                     /translation="MKKRNLSQEKIIDCFRELAEEMDVQQITFQHLAKRLDIKAPSLY
                     NHFKNIRDVKTALTAKLLQELNDQLRRALVGKSGQEALQIYAETYRSFAFSNQAVYEL
                     LISVPHTNEAVLLDGIHETNQIILQIFEAFQLTKEEKVHRSRELRSMIHGYLSLRFLG
                     YFTKEPTIDPEDSYFWMINNFIATLP"
     gene            18556..18933
                     /locus_tag="ACG842_000809"
     CDS             18556..18933
                     /locus_tag="ACG842_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HFQ0247371.1"
                     /translation="MYQVITMFGDNEPWWFFEDWEEDIEEEETFESFEEARQAYEKQW
                     NEIHQNYEYINAKSNFLSAFWNDGDERWCEECDDDLQQYKGLALLKNHQPITVESRKE
                     FYETTNSSGKTKRCERPKQGAWC"
     gene            18866..20938
                     /locus_tag="ACG842_000810"
     CDS             18866..20938
                     /locus_tag="ACG842_000810"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_function: GO:0098847 - sequence-specific single
                     stranded DNA binding [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0051304 - chromosome separation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HFQ0247372.1"
                     /translation="MKQLILAEKPSVAKDLSKVLGANQKHKNYYEGPKVIVTWALGHL
                     LGLKMPEDLNKEWQSWQMDTLPMIPKKLGIKPLPKTGHQLKAIKQLAQRKDVSEAVIA
                     TDAGREGELVARWILEYVHFNKPVKRLWISSQTDKAIKTGFKQLRPAKEYDALYESAL
                     ARAKADWLVGLNVTRALTVKYADNLSAGRVQTPTLALVRDQEKKIEQFRPQTYFTITL
                     QAGKEQAKMLQKNQFALKTQEEAQKLVQLISTSKGTVTEVQEKLKTESAPLPYDLTEI
                     QREANQRYGYSAKKTLSLVQSLYETHKIVTYPRTDSKYLTNDMKSTMKERLQAVSDFA
                     PEVKQYLKNGAIVQQTKVFQDSKVTDHHALLPTENRARYEKLSNEERKIYQMIVSRFL
                     GLFAQPHKVSQTKVTVEFGKERFIFRQNRVVQAGWKGETETETETVKWEKGMRINPDF
                     AIKKELSAPPKPLSEASLLGQMEKYSLGTPATRAEIIEKLIKSELMERTPQGLQVSPK
                     GKQLLVLVNPSLVTPDLTESWEKDLEAIAAGKKQAGTFLNGIEKETKRLVNEIKSSKQ
                     EYQDFSITQKKCPECGANLREKNTRDGKIYVCTNSDCNYRRRKDPKVSNHRCPQCHRK
                     MEIIEGKNGAYFRCKYDGTTEKLLDKKEQKKKMTKHEERRLLKKYAQAEEPEESPLAA
                     ALKAAMKK"
     gene            complement(21149..22096)
                     /locus_tag="ACG842_000811"
     CDS             complement(21149..22096)
                     /locus_tag="ACG842_000811"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFQ0247373.1"
                     /translation="MKVSIVVPCFNEEKTVPLFFDEVEKFKGAHEFEYLFIDDGSKDG
                     TLAAIKKLASAYPYVRFVSFSRNFGKEAALYAGLQASTGDLVTVMDVDLQDPPELLPE
                     MINRITTSDIDCVGTRRSTREGEPAIRSFFAKKFYQLINKISDTEMVDGARDFRLMTR
                     QMVDAVLELTEYNRFSKGLFSWVGFKTEYISYENRERVAGETSWSFWKLFNYSIDGIV
                     NFSDAPLTIASFIGALSCVGSGIAILFIILRALLFGDPTSGWPSMVSIFLFIGGIQLL
                     CLGIIGKYIGKIFLETKQRPVYIIRETEKVKPNELKPHQ"
     gene            22342..22806
                     /locus_tag="ACG842_000812"
     CDS             22342..22806
                     /locus_tag="ACG842_000812"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HFQ0247374.1"
                     /translation="MKIHTPIGFLWLDADESGLTAVSFQPISESESANQRILTRTQEQ
                     FEEYFAGKRKEFDVPLSIKRGTPFQQNVWKALSTIPYGEIRTYKEVAVAVDSPKAVRA
                     IGQANRTNPLPIVIPCHRVIGQNGKLTGYMGNAIDGVEIKRQLLLLEGYLQN"
     gene            complement(22868..23911)
                     /locus_tag="ACG842_000813"
     CDS             complement(22868..23911)
                     /locus_tag="ACG842_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HFQ0247375.1"
                     /translation="MKSLSHPIKSVKDSAVREILPGLLLSFAIAAFSRLLAIYLPQLG
                     GATIAILLGILLGNTFFKQPVLAIGTKFAESKLLEFSVFLLGATVTAQTIAQIGVKGI
                     VFVLLQMSVTIIGAYLIGKKLQFSDTSSLMMAGGNAVCGSSAIASIAPSIHAKEEEKG
                     QIITLVNLLGTVMMLTLPFLGLQLFGGNLIEKSALLGGTLQSVGQVVAGASLLDNSVV
                     EFAMLFKILRIMMLVVVVYLFEKFMHKKQPALFETPTDTQKKRKLPLPWYVLGFVIFC
                     LVNSLFSLPVVIDQSAHFISSWFEITALAAIGLRLDFAKFMKEGTRFLSYGLAVGLLQ
                     TVMALLFIWLIKV"
     gene            complement(24288..25253)
                     /locus_tag="ACG842_000814"
     CDS             complement(24288..25253)
                     /locus_tag="ACG842_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HFQ0247376.1"
                     /translation="MKNFLKIIPIPICGLILGLASLGNLFKDYHHVALGNLTGGISMI
                     LMIFIFVKLFILFEHTKQTLHDPIIASVSPTFTMSLMVICTYFVSYESIAPIVKFIWL
                     CAVIFQVILVFYFNYHHVIKADLSIEAIYPSWFIIYVGFGVITVTAGNFFPLIGKIFF
                     WISLICYAFLLPIIIKRVFFVRNMAHPTRPLITIVAAPGSLCLTGYLKNFAEPNFYLV
                     VALFALSQVLYFVVLSMLPRLFKLRFYPSYAAFTFPLVISATAIFTTVHYFNSLGIYS
                     SVLDFLKVAECLFASVVVFYVLAHYVRFLIKEHSKHGKFVSKKIA"
     gene            25487..26332
                     /locus_tag="ACG842_000815"
     CDS             25487..26332
                     /locus_tag="ACG842_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFQ0247377.1"
                     /translation="MFKLLTTFRAVYETRNFSRAAEVLFLSQPAVSNQIKQLERELNI
                     DLFQRNGRKEMLPTKQADLLYERTLVLLEEWEETQQKLLNEQNEEEVCRIVASHTFAV
                     YLLPQLMKHLIKRFPKVHFEIEITNSHESLEQVAKHESDFGFIEKPLETSGVQRYSLM
                     EDQLVIAGDPKSKLWLVREANSGVFHYTQRYFEEMNIQDKKMIVKSNDVIVALLKEGI
                     GRSILSKRSVPENVPMTPLNFHRYFYFIQRTNLVSDQLLEIAEEILRYNESTETSHSE
                     ENLSW"
     gene            26523..27710
                     /locus_tag="ACG842_000816"
     CDS             26523..27710
                     /locus_tag="ACG842_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HFQ0247378.1"
                     /translation="MEKKLKASNDNRIDYGVILPVFLLCLIGLLSLYVALSHDPNNPA
                     VLKGVGMQVVWYLVGAAAIVVIMHLNSKWIWKLTPYLYGLGLVVMGLLLKFYDQNLAA
                     STGSKNWFRFGAFTFQPAELMKIAYILMMALIVTKHNTQVKERTMKSDFWLIGKLLIV
                     TIPVLALIMAQDDFGTMLVFLAIFGGIFLMSGISWRIIAPVVILAVVVGAGTIFLVTT
                     EGGRDLLYKVGFKSYQFARIDSWLDPFHDTSGMSYQPAQGLLAIGTGGLFGKGFNVSN
                     IYVPVRESDMIFTVIGENFGFIGGAFVIFLYFILIYRMIRVCFDTNNEFYAYIASGLI
                     MMLLFHVFENIGANIGLLPLTGIPLPFISQGGSSILGNMIGIGLILSMRYQNEAPARS
                     SRR"
     gene            27716..28072
                     /locus_tag="ACG842_000817"
     CDS             27716..28072
                     /locus_tag="ACG842_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HFQ0247379.1"
                     /translation="MYTFYWYPKCSTCKKAKAWLDQHGVSYQTVDMIKEVPKASTIET
                     WLETSQVPIRRLFNTSGMKYRELGLKDQVGEFTASKASQVLSTDGMLIKRPLILREDQ
                     LVAIGFNEKIYEGELI"
     gene            28074..28412
                     /locus_tag="ACG842_000818"
     CDS             28074..28412
                     /locus_tag="ACG842_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HFQ0247380.1"
                     /translation="MAGKHLKKKDNLWILFNGKEYCVGLTNEAQEELGDITFANLPKA
                     GQSLKKDEPLIEVEAEKAVNEFASPLTGTVSSVNDKIGEDINILNDSDEMNAWILSFK
                     DVDPKEFDEL"
     misc_binding    28488..28744
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            28807..29841
                     /locus_tag="ACG842_000819"
     CDS             28807..29841
                     /locus_tag="ACG842_000819"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HFQ0247381.1"
                     /translation="MSLIQLTNVKKTFNGKNGNIQAVNDVSLNVEKGDIYGIVGYSGA
                     GKSTLVRLLNGLELPTSGEVIVNDQDITKLKNKELRTFRKKIGMIFQHFNLLWSRTVL
                     ENIQLPLELAGVPKSKRKERAEELLRLVGLEGRGQAYPSQLSGGQKQRVGIARALAND
                     PEILLCDEATSALDPQTTEEVLDLLLAINKKLNLTIVLITHEMNVIRKICNKVAVMEL
                     GKVVEEGDVLTVFRQPKKEVTKRFVQQDIEPEADSTELLAELIKENPSGRLATLTFSE
                     RNANEPVISQAIRRYNIDVNVVYGKIKQVKEGSFGSLTVLMTGETKELDQAEVFIKEQ
                     GVGIEVIHRG"
     gene            29834..30520
                     /locus_tag="ACG842_000820"
     CDS             29834..30520
                     /locus_tag="ACG842_000820"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease"
                     /protein_id="HFQ0247382.1"
                     /translation="MDKSFSETYLDFKQINPETMNQAILDTLYMTLISMVLVTIIGLV
                     LGLILYSIGRKNKPYARIIYGIVSIISNIFRSTPFMILMVLIIPFTKAIVGTMLGAQA
                     AIPALVLSAAPFYARLVEIAFREVSPGVLEAADAMGASYWEIIWKVLIPESIPALISG
                     LTVTTISMIGFTAMAGAIGAGGLGGLAWQEGYQRGNLTVTFVATLIILLIVFIVQGIG
                     DFLTKKTDKR"
     gene            30544..31392
                     /locus_tag="ACG842_000821"
     CDS             30544..31392
                     /locus_tag="ACG842_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HFQ0247383.1"
                     /translation="MKKKIFGFAAALLLTVGLAACGNNSDSKDSANKADDTTLKVGAS
                     PTPHAEILEHVKPLLKDEGIDLEIVKFDDYVLPNQSLEEGDIDANYFQHIPYLNKEIK
                     EKGYDFVNAGAVHIEPMGLYSKKVKDVSELKDGATIITSNSESDWGRIITILQDAGLV
                     KVKDGVDLETATFDDIEENPKNLKFNHTIDPSLLASTYQNDEGDLVAINANFAYGAGL
                     NPLEDAVLLEKDNSPYVNIIATRKGDEDSDKIKKLIEVLHRKEVQEWITDKWGGSVKP
                     VKADAK"
     gene            31589..32362
                     /gene="sufC"
                     /locus_tag="ACG842_000822"
     CDS             31589..32362
                     /gene="sufC"
                     /locus_tag="ACG842_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HFQ0247384.1"
                     /translation="MSVLEIKNLHVSIEEKEILKGVNLTMKTGEIHAIMGPNGTGKST
                     LSAAIMGNPNYEVTEGEILFDGKNVLDLEVDERARLGLFLAMQYPSEIPGITNAEFMR
                     AAINAKRDENDKISVMAFLKKLDEKMALLNMPEEMAERYLNEGFSGGEKKRNEILQLL
                     MLEPTFAILDEIDSGLDIDALKVVAKGVNEMRGDNFGALIITHYQRLLNYITPDVVHI
                     MMEGRVVMTGNADLAKRLEAEGYAGISKELGIDYKEEEA"
     gene            32375..33661
                     /gene="sufD"
                     /locus_tag="ACG842_000823"
     CDS             32375..33661
                     /gene="sufD"
                     /locus_tag="ACG842_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HFQ0247385.1"
                     /translation="MKEKNWLDYLDAVNDFSLSKGEPDWMRTFRQDALAKADELPLPH
                     IDRVKFHRWSLFDVKETQTISETGTIPAFDAMKDNPVLVQQGSWTIFEQLPVELAEKG
                     VIFTDLFTAMIEYPELVQEYYMKKAVNMNEDQLTALHVAFMNSGIFLYVPKNVVIDEP
                     LESLFIQDGASDEHFFKHVLIVADEHSEFSYLERFQTTKEQVAKSSGNIIVEVIAKAG
                     SKIKYSAVDQLGENITSYMNRRGHILRDASVDWAIGVMNDGHVIADFDSDLAGEGAHA
                     EVKIVAISSGRQIQGIDTRVTNKAPHTIGHILQHGVIREKGTLTFNGIGHILKGAKGA
                     DAQQESRVLMLSDKARGDANPILLIDENEVTAGHAASVGRVDPEEMYYLMSRGLHKEE
                     AERLVIRGFLGSVLTAIPVEQVRKELVEVIEGKLNG"
     gene            33658..34893
                     /locus_tag="ACG842_000824"
     CDS             33658..34893
                     /locus_tag="ACG842_000824"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HFQ0247386.1"
                     /translation="MIDAKKLRNDFPILFQDVNDEPLVYLDNAATTQKPKQVLQALAY
                     YYEHDNANVHRGVHTLAERATHDYEQAREKVRAFIHAKETAEVLFTRGTTTSLNWVAR
                     TFGDRYIKAGDEIVLSYMEHHSNVIPWQQLAKRTGAVLKYIELTEEGFLDMEDAAKKI
                     TDKTKIVSIAHVSNVLGVFNPVQELAELVHENGGVIVVDGAQSAPHMAIDVQALDCDF
                     FAFSGHKMCGPTGIGVLYGKRKWLEEMEPVEFGGEMIDFVELYDSTWKELPWKFEAGT
                     PNIAGAIALGHAIDYLQKLGMDNIHRYEEELAAYVLPKLQAIDGLTVYGPQDPNFRTG
                     VVAFNLDGLHPHDVATALDMEGVAVRAGHHCAQPLLKYLEVSATARASFYFYNTTQDA
                     DRLVEAIMATKEFFQHGTI"
     gene            34880..35350
                     /gene="sufU"
                     /locus_tag="ACG842_000825"
     CDS             34880..35350
                     /gene="sufU"
                     /locus_tag="ACG842_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly sulfur transfer protein
                     SufU"
                     /protein_id="HFQ0247387.1"
                     /translation="MGLSKLDNLYRQVILDHSSHPHHRGSLTDSTSKIELNNPTCGDV
                     IELQVDVKDGIIKNIAFDGSGCSISTASASMMTDAVIGKSIEEAEHLTVDFLQLVQGK
                     EVKDPDQLGDAAMLGGVAKFPARIKCATLAWKALGKAMENDGTAAFHHYHKEEE"
     gene            35355..36746
                     /gene="sufB"
                     /locus_tag="ACG842_000826"
     CDS             35355..36746
                     /gene="sufB"
                     /locus_tag="ACG842_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HFQ0247388.1"
                     /translation="MGVPELEEYKFGFHDDVEPVFSTGEGLTEEVIREMSRIKGEPEW
                     MLEFRLKSLETFNKMPMQTWGPDLSDIDFDAIKYYQKPSDKPARDWEDVPEKIKDTFE
                     KIGIPEAERAYLAGASAQYESEVVYHNMKDEFEKLGIVFTDTDSALKEYPELFKEYFS
                     KLVPPTDNKLAALNSAVWSGGTFIYVPKGVKVDVPLQTYFRINAENTGQFERTLIIVD
                     EGASIHYVEGCTAPTYSSNSLHAAIVEIFTRKDAYCRYTTIQNWSDNVYNLVTKRAKA
                     YEGATVEWIDGNLGAKTTMKYPSVYLDGKGARGTMLSIAFAGANQIQDTGAKMIHNAP
                     NTSSSIVSKSISKDGGEVNYRGQVTFAKNSAGSISHIECDTIIMDEWSKSDTIPFNEI
                     HNSQVSLEHEAKVSKISEEQLYYLMSRGLTESEATEMIVMGFVEPFTKELPMEYAVEL
                     NRLISYEMEGSVG"
BASE COUNT        12021 a         6791 c         7870 g        10095 t
ORIGIN      
        1 ttccgttaat aaatctcttt attatcaaca acttaagtta tattttttct ttttataaaa
       61 tacacttatt ataattttgt tttttattga cagtaaaaat aaattaggta atactttaag
      121 taaacgcttt ctaatttgtt acgggaggaa tacacttgtt aaaaaaacgt ataaaataca
      181 gtgcgctaat gatgaatctc cttatgctca gtaccccggt tttaatttct gtcgttgaag
      241 ttgctgctaa cgaacaacag tctgtgaacg aagaaaatgc acacgtgaac attgaaggga
      301 tgtcaacaat ggaggaaata ccaagaacta gtgaagatat ggatacggaa gaaggaaatg
      361 atcctatgat gaaagaatca gaaaatgaaa agacaaaaaa gcaatctgaa gaagcaaatg
      421 aagagtggaa aggggttgtt ttcggagaaa gcacaagtgc tagtaggtat gccattgaac
      481 cattagaaga cggcgtgcgg ctctcttcca cgaataacgg cggaaaattc cagtcaagcg
      541 gatcagatgg aatgacctat tattatcaag caatccccaa agacatcaac tttaggatgc
      601 gggcgacgat cgaaatagaa tcttggacct atacgaatgc ccaagaagga tttgctttaa
      661 tggtaagaga cgcagtacca aaaaatcatt tgtttgggca ggcattttat tcaaattcat
      721 ttgccatact aggcagtcgg atcgaatatg tctgggactc ggatagacaa gaagtggtca
      781 acacgagcgg cagcaaatat acgatgcgac ttggattggg gacacgggca atcacaggaa
      841 tatcatctga agatccgcaa gctgcgccag ctcctgggag tgtctcagtg gaaacaacac
      901 ctttagagac ttcagcaggg ttcttggaaa aagaaacagg ttcttataat atattcggaa
      961 acggtcatgg gaatctgttt ccggccacga actcgattac taaactggac gtagagatga
     1021 aaaaaacgaa tactggttta gaggcatatt attacgatcc tgaaacagga gaagaaatgg
     1081 cttctgacat catgtatgac tgggaaaaat tatacgcttc agatgaatcg gttatttatg
     1141 caggatttgc tgcagcaagg aatatgacga tcaaggtaga aggaatcgaa gttgcttact
     1201 cagatcctgc gacagatcct cctgggcaag aaagacctac tcgcttgatt gatccgattt
     1261 acactgtgac ctctagtaat cattcaggaa accaagatca tactatgtcc tttagagcaa
     1321 atgctgacgg attattgaca attaaaaata aagcaacagg agaggtgctt aagaacgcaa
     1381 aaaacttggc tatcactgcg aatcgggaat ttgattatca gacaaaactg atggaaggaa
     1441 cgaacgaatt tactttcagt tttacgccag ataaaaacta tccgccagaa gaatacgtag
     1501 agatgactag ttatgaaaca aaagagattc ttcatacagt ggactaccgt aaacctaaat
     1561 attcgacagt ttatgtaaca ccagaagggt cagatgcagg aaacggaagc agacaaaatc
     1621 cattatctct gacacaagca ttgagatacg catatgctgg gcagacaatc gtcatggcgc
     1681 ctggaactta ttccttcgaa agaggattga cgattccaaa aggcgttaac gggacaagag
     1741 aaaacccgat tcagttgatt gcagagaaaa atccagaaaa caagcgtcct gtacttgatt
     1801 ttaaaggaac tggaaatggg atgacactgt tcagtcacta ctggggttta cgagggtttg
     1861 acattacaaa tagtgcagct atgcaaaaag gattacagat ttctggaaat cataatttga
     1921 tcgaagagat tcatgcttat cggaatggaa atacaggaat ccaaatcagc ggtagctcaa
     1981 atgatccttt cgaagcatgg cccgcgcata acttagtcaa gaactgtaca tcttttgaaa
     2041 atgccgatcc aggatttgaa gatgccgatg gatttgcggc caaactgaca ataggagaag
     2101 gaaatgtatt tgacggttgt atcgcgtatc ataatgcaga tgacggttgg gacctttttg
     2161 ctaaaaatga aacaggttcg atcggaaaag taatcatcaa aaactcagtt gcttatcgaa
     2221 acggctgggt accaggaatt gagggagaag gtaacggtaa tggattcaaa atgggcggtt
     2281 cttctttaac gggccctcat gaattgatca atagtttgtc tttcgaaaat cttgcgaaag
     2341 gaatcgattc caatagcgga acagatatta tcgtcaatag cagtacaagt tttaataacg
     2401 gttcatataa tgtggctctt tatacctctt cagcaggaaa cacagactac catgcttctg
     2461 gtatcttatc tttccgaaca gagaatcttg agatgagaga gcagatccgc ccattgaatc
     2521 aaaacgaaga tcaaatctac cactcgttga actactattg gaatgaacga gagaaacaat
     2581 cagaaaatac tcttggacat accatatcaa aagattggat cgagtctcta aatacatgtt
     2641 cgaaagaagg tcagcagcct gttacgcgct atgaaaatgg aagtatccat gttcatggac
     2701 tgatgcagat taaaccgggg aatcgtcaaa cagaaaagga aagagtggat ggtcttccat
     2761 tatctgtagg tgctattttt gacggagaaa cctcaccgtc ttcggaatat ccagagtatg
     2821 caatcgtaca agaaccaaat cagacaaaac caaatgaaca aaataatccg attattatac
     2881 aagaagcaaa tggtgtggcg gagaatgcag aaggaaatat cattgaactg aagcctttta
     2941 cagtttttgt gtcaactttc acaccaaatc aaggaacgcc tgtctttgaa gatatgcgtt
     3001 atttcccaga aaagatccaa atcgttgaaa cacagtcaag tgctcaattt tctaatcata
     3061 ccgctatttt ttcagcacct tcaaaaggag aatatacgct agaagtcact tatcgttcgg
     3121 aaatatttga tggggaaaaa tggtctgctc atacagaatc tgttacccag caaaaagcga
     3181 tcactgtgcg agaaactgcc gatgtgcccc cgggaaatga caaaccagaa agagaaaatc
     3241 caggaaatga atcaccaaat ggaaaaccaa atggaaaacc ttctcatccg gataggacag
     3301 caaaagaaag tgaaaaaaat ggcactcaag gactcccaaa aacgggagaa tttaataacc
     3361 cgttgctagc attagctgga ggaattttgt taatcggtgt agttgtttat gtcatgaaac
     3421 agcgaaaaaa ataagtggaa tttctccttt tatgatgtaa aacaattgac tgaggaaagt
     3481 aaaaatagaa ggaaatgaga ccaatagatg tctcacttcc ttctatcttt ttatagatga
     3541 ggttctcctt gctgcttttg attggctgtt cttattactt gatgcaggac agctttttca
     3601 caatctctcc actaaacggt gttcaatcag ctgaccctcg gtattagctc aacaaacggc
     3661 aaaatatgag gaactatttt tccgtttttt tctctaatac tcaggtcagg acgctgattt
     3721 cacaacctct cgataaacgg tgttcaaaag ccagtttctc ggtagtaagt caaatttatg
     3781 caaaaatgtg gaaagccatt ttcacaaaat ttttcttact acttgaaact aagcagcttt
     3841 ttcacaacct ctaatacatg gtattccaga agtaacttct tcgaaaaata agccgaaatg
     3901 tcacaaaaat ttgagaagcg attttcccaa attccttctt atttcttgaa gctgaccact
     3961 tctggcacaa cctcttacca gattcgcact cttttttctg gcgctagata catagcgtct
     4021 tgttccgtga taccaaatgt ttcatagaat tcgtcaaggt tttttggttg cacgtttgct
     4081 cggagtttag caggggcatg gacatcgatt tgcagtaaaa gttgttggta ttcttttttt
     4141 gcttttgtac gccaaatcgt tgcccaattg acaaagaatt cctcgataga aagatctgat
     4201 tccgtttttg ctgcttccaa tgcacagctt agaccgcctg catcagcaat attttccgaa
     4261 acggttaatt ttccgtttac ttttccgtca gcaaaaggca gaccatcgaa ttcttcgatc
     4321 attgcttgtg ctttgttttg aaattgtgta agatcttctt ctgtccacca gttgttcaag
     4381 ttgccatatt cgtcaaataa agcgccgtta ttatcaaatg catgggaaat ctcatgagcg
     4441 ataacagctc cgattccacc ataattagca gagcttgatt gttctagact gtaaaatggt
     4501 gcttgaagga tcgctgccgg aaagacgatc acattccgaa atggatgata ataagcattg
     4561 accgtgtcag cactcatttc ccattctgaa cgatccactg gtttgttcca tttgctgaaa
     4621 cgatctttta atgtgagttt gctgaaaaca agagtatttg aaagcaaagt accaccctga
     4681 tctgcaggta ctgttttgaa ctggtcgaat aatgctggga tcttatcggg gtagcctact
     4741 tggataccta agttatttaa ttttatgacg gctttttctt ttgttgcatc cccaagccag
     4801 tcattttctt ctagacgttt tttataaacg gcgatcattt ttttcaccat ttgttctacg
     4861 tcttttttag cagcttcgcc aaaatatttt tttccgtagt aatcgccaac tacttgatcg
     4921 aattgtccag aggcaaggta atacgctgct tttttctgag gcattgcttc gtctgttcca
     4981 gacaatacgc gagaatagat tccgctgact tggcggaatt cttctgatag ataaccacta
     5041 agagaacgta ctgttttaat gatcatccag cttttgaata acggaaaatg agcttctgtc
     5101 aggatgtcat tcaaatgatc gaagtacact ggatcagtta cgatcacttt atctggagtt
     5161 gtcccaatca aacccgtgac aagttttttc aagtccagtt tatctgtgta tttaataaat
     5221 gtatcaaaag attgcggatt atacattttg ctgtaatctg cgttttcttc tgcacttttt
     5281 acatgaggag caatcaattt atcaaattgc actgcttgtt ccgtgattgc ttctgcctga
     5341 cttttttctt tgccggcaag ctctaataac tgaacagcca tatcaaagaa aacgcctatt
     5401 agttgtgcac cgttgggatg gtctgatgca taatacgtct tatctggaag gaataaagaa
     5461 ggcggataag caaagaaggc gttcgtttgc gcattcttca tatctgcatc tacatccaga
     5521 ttaaatggca atggtaatgt atccaacagc cattctggaa attgcttatt caattcgtcg
     5581 aaagactgca atgattcaac tttttcgatt ataggttgaa gcggttgagc atgcaactca
     5641 tttcttcggt cataatcgtt tgccagttga taatatgcaa ggaaatgttt cattttttct
     5701 gaagacactt tttctggatg ggcaagcaga tcgtctgttt ctcccatcaa aagttggtcg
     5761 atcccgtcaa ctaaatcttg gaaaccgcct gtagctggtt tatctgcagg gatttttgct
     5821 gttttttgcc attcttcatt gactgcttca tagaaatctt gtttcaatcg ttctttgttc
     5881 attcatcgat cgctcctttt ttgtttcgtg tgcccattat cttacaaaat gaaggaatta
     5941 acaaatagaa cataaaaaat tgaaaattcg aacaatcaaa ctgatttgtt tttttcaatc
     6001 cttcacttat aatgaaaaaa aggaggcgat cccaactgaa tcaagtagtg gataaacctt
     6061 tatatttcaa agtagataaa aaaattaaaa aattagccag cactcagcag ctgcagtcaa
     6121 gaaaaagtaa attgcttttt ttagctctgg tttttgaaga tcaaagttat gtgattatcg
     6181 atcaatcagg acatccggta gaatattctc cagcaaaata tacttatcaa gagggtcttt
     6241 cttgtaaaaa atggaagctg atcaatgaag agccaatcga gttatctaga tggatcaaca
     6301 gaaaagaaga tattcccgtt ttgatcgaag aaaaaaggtc aggaaaggaa ctcgcaaatt
     6361 gctgggtggg attgccagaa gaacgttttt tgaggtataa gaaatgggct accccctcag
     6421 gctatctttg tggtacttac gcggcagcag ttctccttgc ctattatcaa gactatcgta
     6481 aggggtggat gcttccaaat gagattcgca agaaaaatac ggctgattcg ttggttttga
     6541 cgaaggcatt gcgtagccag atccaaccac taggacttcc tacgattcct tttcaggtta
     6601 gtacagggat ttctagtttt ttaaagaaga atggcaatca tgagcgtgca cgagcaactc
     6661 ttctaggttc atggcagcga gctacaaaac gtatacgtga aggcaaacct gtgatgattg
     6721 gtattttgaa agtacttggc agtacatatg gcaatcattg ggtgacagcg tacgcttatt
     6781 ttgaaactga aacaggagaa cgttattaca aagtacacga caactggggc gattatcata
     6841 aagtcattcc tgccagttgg agtaacggaa cagtttcact tccttaagaa tttgctgaaa
     6901 ttaacagaaa atcttggaaa aatatgttaa actatcgaag aatagagaag ttttggggag
     6961 gactacatga gtaataagta tcaggatgac acgttgaaga tttttagttt gaatggcaac
     7021 cgtccattag ctgaaaaaat cgcgaaagtg ttcggaacag aattaggtaa aagcgtcgtg
     7081 aagcaattta gtgatggcga gatttccatc aatatcgaag aaagtattcg aggggatcat
     7141 gtgtatatcg ttcaatcaac gaatgcacct gtcaatgatt actatatgga actactgatc
     7201 atgatcgatg caatgaagcg agcaagtgcg aaaacgatca atgtcgtatt gccttactat
     7261 gggtatgcta ggcaagaccg cacggcgaaa cctcatgaac cgatcactgc gaaattgatc
     7321 gctaacttga tcgaagaagc aggggcaact cgagtactga ctttagatct gcacacggtt
     7381 caagtacaag gattctttga tattcctgta gataatttat ttacgatgcc gttgtttgct
     7441 cattattacc gtcaattagg tctgacggga gatgacatcg tagttgtttc gccaaaaaac
     7501 agcggtgttc agcgtgcacg tagtctttca gaatatttga acagcacatt agccatcgta
     7561 gatcatgcag atgatgcttc aactgagggt ggttatgtga tcggtgatgt tcaaggaaag
     7621 acatgtatca tggtagatga tatcttgaac acaggagcaa cgcttgcacg agcagcaaat
     7681 gtattaaaag aaaacggcgc aaaagaagta tatgcctgtg cgtcacatgg attattgtcc
     7741 gatcatgcga aaagtatttt agacaatgca ccaatcaaag atatttgtat cactgattct
     7801 gttttcacag aagaaaatcg tcatccagaa aacttaacaa tcgttacatg ttcagaatta
     7861 atgggtgaag cggtcaaacg catccatgaa aatacaccga tgagtccgtt attccgtctg
     7921 gaagaaaaag aattcgaata aacaaaaaaa agagggaaag aagttgtgac agaagtgagc
     7981 agcttcaagg agctgcgaca agacttttgt cacagctcct tttttctatg cccatgaaac
     8041 gtcctctaga caaaaatgag ctcttctttg gtttgttcgc tattttatgc tagaatgtgc
     8101 ctagcaataa tttggaaggg atgaatgaag tgaaaactat ttatctggat catgcggcaa
     8161 ctacccccat gcatccacaa gtaattgaag cgatgacggc aatcatgcaa gacacgtttg
     8221 gaaatccttc aagcatacat ggatttggtc gccacgcgca tgaaaaattg gaaacagcaa
     8281 gacaaatcat tgcagacagt ctgcaagcaa aacctcacga gatcatcttt aacagtgggg
     8341 gaacagaagg ggataataca gctatcttag aaacagcgtt ctcgcgtcaa aaagaaggca
     8401 gacatctaat cactacagct atcgaacatc ctgctgttct taataccatg aaatatttag
     8461 agacaaaggg gtttgacgtt acatatctgc ctgtagatga aaaagggaat ctgtcgatcg
     8521 aagaggtcaa aaaagcgtta cgagaagata cgatcttggt ctctgtgatg tacgggaaca
     8581 atgaaatcgg aaatttgatg ccaatcaaag aaatcggtga acttttagcg gatcatccag
     8641 cttatttcca tacagatgcg gtacaagcgt acggaaacca aacgatccat ccagaagaat
     8701 taggaatcga tttattaagt atctctgctc ataaaatcaa tgggccaaaa ggcgtcggtt
     8761 tcttatataa acgagacgga gtaaatatcc ctgcattgct tttaggcggg gagcaggaag
     8821 aaaagcggcg tgcaggtaca gaaaatttag cagggatctg gggtatggca aaagcagttg
     8881 aactgctgtc acctgaagaa cgtgaagcac gtaacaacaa atataaagga ttccagcaat
     8941 tgattcttga tacattagaa gcaaacgaaa tcccttatca agtaaatggt gatttaacga
     9001 ataaactccc tcatgtgttg aatcttcgct taattggcga agcaaatgat ttgctactga
     9061 tgaagcttga tcttaatggg gtcgctattt ctactggttc agcttgtaca gcaggaaata
     9121 tcgagccttc acacgtatta gaagcaatgt atggagaaga tacaccgata ctaaaagaat
     9181 ccatccgtat cagctttggt ttgggaaata ccgaagacga catccagtat ttccttgatc
     9241 aattagttcg tgctgtcaaa aaataactag tgaagtcatt cttatatgaa cagatgtctg
     9301 agagaacgcc ttcatttttt agggaactta actgaaaaac ctagtaaaaa tcaggataaa
     9361 cttttataat aaagagaaac taattttatc gaggtgaaaa tcatggcatt tgaaacaaca
     9421 gcaacggtac tgggcgcacc aattagctat cgtttgcatc ctgatgcaaa aagatataca
     9481 ttgcgcgata atggatttac agaaacaaac ggaggcaatt atcaattgat ccgaccatta
     9541 gatgccacgc cgcaaagcaa agaaggattc aaattaaaaa tcactgtatc aaaagatatt
     9601 caaacattga aaatgtcgat cactacagca aatggattga aagcagtcaa tattttcaaa
     9661 gatccaaaac agaaaatgag ccaagataaa ttttattttc tgatggatgg gatgattagc
     9721 cgtgggttat tcgaaaaagt cgaatgatct gtggatataa aaagacaact agttttgaaa
     9781 cggcctattg ttagatgact aactttgtaa ggacgacgca aattagttgt cttttttatt
     9841 tttaaattta acacatgttg ttatttttaa tattttgctg atttattgtt tctaagtaaa
     9901 gaaatttaaa ttgaaacgat tgttatttag ggaaaaaggt attttttgaa aaaagaatcc
     9961 cgtataatga ttgttgtgca aacgctatac aaaatgaaaa agaaaagagg gatgtttatg
    10021 gaaaatgaac agtcggtcgt tttaacgaat tggaagagaa attatttgtt ctttttatcg
    10081 ggacagtttt tgtcaggaat cactagtatg gtcgtccaat atgcgatcat ctggtatttg
    10141 acaagagaaa ccggttcggc gacaattttg agttttgcta ctttactagg aatgattccc
    10201 atggttttac taagcccatt tgtaggaccg ctagtggatc gctgggataa aaaagctctt
    10261 ttgattgtca cggatattat tgtagcgatt ttcgcgctta ttttagcagt agtcggaacg
    10321 atttcagaat ccttcccgat ttggctagtt tttgtgtctt tgtttatgcg ttcggtcgca
    10381 caaacattcc aaatgccaac gatccagtcg attatgccga caatcgttcc atcttctcac
    10441 ataacaagga cgaatggaca gttaggaatg gtccaatcag cgaatttcat cattgcgcca
    10501 gctctaggtg cggctttgtt ttcagtcgtt cctgtcaatt accttatttt attagatgta
    10561 ttgggggcag tttttggtgt cggcttattg atttttgtga agatcccaaa agtttctcct
    10621 gaaatattgg aagttccgct tactattttt aaagatgcaa aatttgggct gcagcaattg
    10681 atggataata aagggttatg gtacataacg atcaatggtg catttgtgat gctgttgttc
    10741 atgccagcaa tcagtctgta tccattgatg acgctagatt actttggtgg ttccgttgga
    10801 caggcgggag cagtggaagt agtctacgca gtcggaatgc tcttaggcgg tgcactgatc
    10861 agttttatag gaacatggaa agaccgaatg aaacctatta ttatagccta catcattatg
    10921 ggcctgacga tcggtgcaag cggattggtt ccaaacgata gtcaagggtt cttgtacttt
    10981 cttatcttga atgcaggagc aggttgtgca acgccatatt tcaatacact gctgatggca
    11041 atgatccagc agagttatga atcgaatgtt ttaggtcgtg tcttagggaa cttcaactct
    11101 ttgatgaacc tggcaggtcc aattggatta ttatttgcag gaccattagc tgatcgttta
    11161 ggggtagaaa aaatgttctt gttttccgga atcgggattt tattatgcgg aatcgtttta
    11221 tttttaacgt cagctgcccg aaattatgat aaagaattac agaaaaaact ggtaaaagaa
    11281 caccatgagc aaaaagacga atagtatgga atggagttgt gacagaagtc ttgttacaac
    11341 tccatttctg atcctaatgt tcctgtggca ataagcctac tcaaatgaaa aatatgagta
    11401 attatcttta tttgtttaat acttgatata gggcagtttt ttcacgacct atcgtacttc
    11461 ctctttgctt catagatttc tttgatttgt tgctcagata acggagtgcc aaatggtagt
    11521 ttttccttta agagttctct ttccggtgcg tctacgccta cattgacatc gttctcgata
    11581 ggtacgctgt aatgatggat atgtccatga agattgcgga tagtgtcgtt aggcccgagc
    11641 aacatcggat aatgagtcaa gtaatattgt tgatggtcga atttaacgat ggcacctaca
    11701 tcataaaaat aaaatttggg acttccgtca gtaagcctag gatcattttt ttctaaataa
    11761 cggaaaaaag cgcgactgtc atggttccct ttgatcaagt ggattgttcc ttgcagttga
    11821 gtgaccagtt ccaaaatttc tgcatttgct ttttcatatt gcggatgcat agcaaggtcg
    11881 cctagatgat aaacatggtc ggtttctttt attactgcat tccagctagt gatcaactgt
    11941 tggttcattt catccacatt tgcaaagagc cgcggagcaa agtcgttttt tcctaataag
    12001 cgttcatgga aaaagtgcat atcactgata aagtaattca tgtatctttt gcctcccttt
    12061 tttcttagag taaacaaaat cacttatctg agcaactcat aacatctaaa aagaaaaggt
    12121 tgtaaatgag acgtgcagct ttaagcgata agaagcaatt ttgaaaaata gtttttcaca
    12181 atttataaaa ttattcctta ttgccgaaag gctggccctt tttatgagta tgccaatttt
    12241 ttatatcgaa tgctcaaaag atgagtgatt ttttggttga ctatcatgtt tgtgtactga
    12301 ataggtccag tagaacagta tttttacgac ttgataatta agggatgaaa ttttcagaaa
    12361 tatgacgtat aatgtaaaat actgaaaagt tgcgaccttc aactcgtaga tgtttcagaa
    12421 tctaattgaa atgatggtga tatcatgaca gacaacagca aaacacgcgt tgtcgtaggg
    12481 atgagcggcg gtgtcgactc atctgtaaca gcacttttgt taaaggaaca aggatacgac
    12541 gtgatcggaa tattcatgaa aaattgggat gacacggacg aaaacggtgt atgcacggcc
    12601 acggaagatt ataaggatgt agcgaaagta gctgctcaaa tcgggattcc ttattactct
    12661 gtgaattttg aaaaagaata ttgggatcgt gtatttgaat atttcctagc cgaataccgc
    12721 gcaggacgta cacctaaccc agacgtgatg tgcaacaaag aaatcaaatt caaagcattc
    12781 ttggattatg caatggatct tggcgccgat tatgtagcta ctggacatta tgcgcaagtc
    12841 actcgcgatg aaaacggcgt ggtccacatg cttcgtggag tagacaataa taaagatcaa
    12901 acttacttcc ttagccaatt atcacaagaa cagctttcaa aaacgatgtt ccctctaggc
    12961 ggcatggaaa aatcagaagt acgggcaatt gctgaacgcg caggccttgc gactgctaag
    13021 aaaaaagatt caacgggaat ctgctttatt ggtgaaaaga acttcaaaca atttttgagc
    13081 aactatctgc cagcaaaaaa aggaaatatg gtcactcttg atggtgaagt caaaggacaa
    13141 catgctggct tgatgtatta tacgattggt caacgtcaag gactagggat cggtggcgga
    13201 ggagattcgc aagaaccctg gtttgttgta ggaaaagatc tagcgacaaa cacattatat
    13261 gtggggcaag ggttccatca tcctgcattg tatgccacaa gcttggatgc aagtgaaatc
    13321 cactttacga cgaatgaacc aatgccaaaa gaattcaagt gtacagcgaa attccgttat
    13381 cgtcagcaag acgttcctgt gacagttcgc ttattggatg ataatcgtgc agaagtcgta
    13441 ttcgacgaac ctgtacgtgc aatcactcct ggacaagcag ttgtatttta tgatggaatg
    13501 gaatgtctgg gtggtggttt gatcgaccac gcatatcaag aaacaaaagt tttacaatac
    13561 gtttaagcga tattaaatga atcaaaacag ctatggctaa caaaagaaac accttttctt
    13621 ttggatagca tagctgtttt tttgcaataa aattaatttt tattcatttt atacttgaaa
    13681 tacttgtgta aaaagttcat aatatccaat aatatgaata aagttgcttt ttgttaagca
    13741 agagcagttg caaaatagaa gggaatcttt tgcaagtgat ttgttcaatt ttataagtga
    13801 gggtgatgga aatggaaaag aaatcgacgg gtattagcaa atggggactg gttgctctgg
    13861 ttgtcagttc gtcaatcgga agcggtgtgt ttgggatcac aagtgatctg gctagttctg
    13921 cagctccagg gccagcgatc ctttcatgga taatcgttgg gattggtatt ttagctttgg
    13981 tattatcgct gaatcattta ggagaaaagc gtccagatct agatggtggt attttcggtt
    14041 acgcggaagc ttcatttggt aaattaggtg gcttcatcag tggttggggt tactggttgt
    14101 ctgcgtggct gggaaatgtt gcttttgcaa cgatgctgat gagcgccatg ggagaatttt
    14161 tcccgatatt caaaggtgga caaaatgttc cttctatttt atttgcaagt atttttattt
    14221 ggggtctgac tttattagta aacaatggag ttgaaggagc gagcgtcatc aatacaatcg
    14281 tgacgatctg taaattagtg ccattattcc tatttttaat atttgtattt atcgcattta
    14341 aagtgaatat attcactgct gatttttggg gtaacgtatc tgataatcta gtgaatggaa
    14401 cagggcagca aggaacgatc tggttgcaga ttaaaggttg tttgatggtt atgatgtggg
    14461 tctttgtagg gatcgaaggc gcaagtgttt tagccaaccg cgcgaagaaa cgttcagaag
    14521 cccagcaagc atcgatcatt ggtctgcttt gtttattgtt tatctatatc cttgcttcga
    14581 tcttgccata cggcgtctta tctcaagaag agttagcaac tatttcacaa cctgctatgg
    14641 caaatatttt aaaaggaatc gtgggaaatt ggggagcagc gctgatcaat atcggcttga
    14701 ttatttcgat cattggttct tggttgtctt ggacgatgct cccagcagaa acaacgatgt
    14761 tgatggctcg agacggtctg ttgccgaaag gttggggaaa aacgaataaa aaaaatgccc
    14821 caacttattc gttagttgtt actgcaatct tgactaatct attcttattg actttcttag
    14881 ttactgatta tgcctatcaa tttgcctatt ctttatgtac agcggcaatt ttgatttgtt
    14941 atctacttgt cgggatctat caagtccagt tttctttaaa agaaggaatc aaaaaacaag
    15001 tagtgatcgg tgggattgct gtagtttttg aattgatggg catcatttta gcaggatttt
    15061 cttatgtcct tttatgtagt attgcctatc ttcctggttt ttgcttctac tggatcgctt
    15121 gtcgtgaaac caaacatcaa attacgggaa aagaaaaagt aatgattggg ctgatttgtg
    15181 tggccgcact gctttcaatc gggttgcttg ctgtaggctg gatcaagata taagggggaa
    15241 agaaacatga aaatcgcaaa tttcggtgta gaagaatggc taaataaatg ggaaaagaaa
    15301 gcggtatatg atattgcaca aagctcgatc gaagcattga cattagacga actcgttggc
    15361 ttagatggga ctagcgtaag taaatttttc gaaaaacgca aaaacgaacc attggactat
    15421 ggctggatcg aaggatcaga tgttttcaaa caagaagtcg cttctcttta tcaaaccgtc
    15481 gatccggaaa acatcttaca aacgaatgga gcgactggtg ctaatctttt agctctatac
    15541 gcgttagtgg aagcaggaga ccatgttgtt tcgatgcttc ctacttatca gcaattatat
    15601 gacatcccca aatctttagg tgccgctgtt gactttgttc atttgaagga agaagaagat
    15661 tggcagtttg atttagaaca gctagaagcc ttagtcaaac ctgaaacaaa aatcatctgt
    15721 ttgaatagtg caaacaatcc tacaggcaca ttgctagatc ggaaaacttt agaaaaaatc
    15781 gcagaaattg ctcgtacagt agatgcatat gtattgatag atgaagtata tgcgccttta
    15841 acggataaag gtgaattctt gtcgatcgta gatgtctatg aaaaaggtat agcaaccaat
    15901 tccctatcca aaacctactc cattccaggt attcgtattg gctggacagc tactggacct
    15961 gagttggcag aagtttttcg aaagtatcga gactacacga tgatttgcgg cggagtcctg
    16021 tcagatgatc ttgctgtaca tgcattgaag aataaagaaa aaatattggc gcgaaacaaa
    16081 aagatcatta cagagaacct tgtgatttta aaacaatggg tagccaatga accgaaagtt
    16141 gaacttgtcg ctcccaatta cgtctctact tctttcatta aattagccat cgaagaagat
    16201 gaccagacat tttgtatcaa tttattagaa gaaacaggag tactattagt accaggaagt
    16261 gcctttgatt taccgaagca tgcccgacta ggttactgtt gtaaaaaaga aaccttggaa
    16321 aagggattat tcctcttatc tgagttcttg aaaaaacagg cctaaacaaa aacactgcaa
    16381 ggacagtctg tgacaactgt tcaagcagtg tttttcattt acgcttattg tctatgagaa
    16441 ggagagtgcc ctgaagctat ggagactcct gaagcttttt cttctgcagt ttcttgctga
    16501 tggatctctt gtgtattgtg tcgtacaagc aagaagttga ccaaaacaag aagagaagtc
    16561 gaaagaaaga cgccgcgata accaaatgca ctggaaacac tggagccgat cataggccct
    16621 actacgttac cagttgcttg gaaagattgg ttataactga agatccgtcc agcagcttga
    16681 tgaggcgaat attttgtgat caatgcttgg acagcaggga gcagacaagc atcagagata
    16741 ccaatcaaaa aacgtaaggc agctaattgc caaacattcg tgacgaatgc cattgggata
    16801 taaaccagaa tcgcaaaacc caaaccaatc gcaagtattc tttcactccc aatcttgtca
    16861 cctaagcgac caaatcttgg agcggctatc aaagtggcaa tcccaggtat cgaagcaatg
    16921 attccgctga tcaacgtaac attcccatgt ccatgcagca attggcgaat gtacagactg
    16981 atgattggac taatggaatt gttggatgct tggatgatca tcgttgtgac gaacataccg
    17041 atgacgacgt gtggatattt caagtctttg aaaatctgtt tggcagaaac catgttttct
    17101 tttttcactg ggacaaattt ttcatggacg aaaaacagac tcaacaagaa gacgaaaaat
    17161 aagatgatcc ctgtaatgaa aaaggtaggg cgatagccaa acgctgaagc agagatcccg
    17221 ccaagaagcg gtccaagtaa tgttcctgta acagaaccag ttgctaaggt tccaagaact
    17281 tgaccacttt tttcttttgg cgtcccagtt gcgacaaggg cagtggcatt actgatataa
    17341 ccagaaaaaa tcccttgaag cagacgcaat gcgactaatt gatacacact agtcacgcaa
    17401 cccatcaaag aaatgacgat tgccattcct aatgaagcac gcaacagcat caattttctt
    17461 cctttttggt cggctaattt tccccaccaa ggagagataa gcgtagtcac caagaaagta
    17521 gatgaaaaag tcaatccgct ccaaaagttc aattgtgacg tactataatt acctaacgta
    17581 tctatgtaaa gtgacataaa aggcatgact aaactaaatc cgataccagc cataaatgtt
    17641 ccaaaccata agacaaacaa gttttgttcc caagtttctc ttttcctaaa taatttttcc
    17701 tttattcttg cagccaatta gactccttct ttctgaaaat tttcatctac agctctttat
    17761 tataaactac aatatgtagt ttatcaatga aagtctcttt tttctctttc tttttcgtgg
    17821 taaactgaaa gagtgaagga tttattttat gagaggaaac atggaaatga aaaaaagaaa
    17881 tctgtctcaa gagaaaatca tcgactgctt tcgagaacta gcagaagaga tggatgttca
    17941 gcaaataacc tttcagcatt tagccaaacg cttggatatc aaggctccct cgctgtataa
    18001 ccatttcaaa aatattcgtg atgtcaaaac agcgctaaca gcgaaacttc tgcaggaact
    18061 taatgaccag ctgcgacggg ctcttgtggg aaaaagtggt caggaagcac tgcagatcta
    18121 tgcagaaact tatcgatcct ttgctttttc aaatcaagca gtgtatgagt tactgatcag
    18181 tgtaccgcat accaatgaag ctgttttatt agatggaatc catgaaacga atcagatcat
    18241 tttgcagata tttgaggctt ttcaattaac gaaagaagaa aaagttcatc gaagcaggga
    18301 acttcgtagt atgatccacg gttatctttc cttacgtttt ttaggatact ttacaaaaga
    18361 accaacaatc gatccagaag acagctattt ttggatgatc aataacttta tcgctacttt
    18421 gccttaaaca atcagattca tttgtaacaa ttttaagatg ttcattaaat gaccctttta
    18481 taagagaaaa ttcttgcaac tttcttgaaa agtgactaag atgagtctag aaaagctgaa
    18541 agaaggagca cgagtatgta tcaagtaatt accatgttcg gggacaatga gccttggtgg
    18601 ttttttgaag actgggaaga agatattgaa gaagaagaaa cattcgagtc tttcgaagaa
    18661 gcacgacaag cgtacgaaaa acaatggaac gaaatccatc aaaattatga atatatcaat
    18721 gcaaaaagta actttctcag cgccttttgg aatgacggag atgagcgctg gtgtgaagag
    18781 tgtgacgatg atttacagca atacaaggga cttgcgttgt tgaaaaatca tcagcccatc
    18841 acagtagaaa gcagaaagga attttatgaa acaactaatt ctagcggaaa aaccaagcgt
    18901 tgcgaaagac ctaagcaagg tgcttggtgc taatcaaaag cacaaaaatt attatgaagg
    18961 acccaaggtg atcgttactt gggcccttgg tcatttatta ggattgaaaa tgccggaaga
    19021 tctgaataag gaatggcaga gctggcaaat ggacacgttg ccaatgatcc ctaaaaaact
    19081 aggaatcaaa ccacttccta aaacaggaca tcaattaaaa gcaatcaagc agctggctca
    19141 gcgaaaagat gtctcagaag cagtcattgc gacagatgcc ggaagagaag gggaacttgt
    19201 tgctcgctgg atcttagagt atgtgcattt caataaacca gtgaagcggt tatggatctc
    19261 ttctcaaaca gataaagcaa tcaaaactgg attcaagcaa ctaagacctg cgaaagaata
    19321 tgatgcattg tatgaatctg ctcttgcccg ggcaaaagcg gactggctgg taggattgaa
    19381 tgtgaccaga gccttaactg tgaagtacgc ggataattta tcagcaggcc gtgtgcagac
    19441 accgacacta gcattagtga gagaccaaga aaagaaaatc gagcagttcc gtccacaaac
    19501 gtatttcacg atcacgttgc aagctggaaa agaacaagcc aaaatgcttc aaaaaaatca
    19561 atttgcttta aaaacacaag aagaagcaca aaaacttgtt caattgatca gtacaagcaa
    19621 aggaactgtc acagaagtcc aagaaaaatt aaaaacggaa tctgcgccat tgccttatga
    19681 tttaacagag atccaaagag aagccaatca acgctatggc tattctgcaa agaaaacatt
    19741 aagtcttgta caaagtttgt atgagaccca taagatcgtt acttatccgc gtacagacag
    19801 caaatatctg acaaatgaca tgaaaagtac gatgaaagaa cgattgcaag cagtcagcga
    19861 ctttgctcct gaggtcaagc agtatttgaa aaacggagcc atcgtccaac agacaaaagt
    19921 attccaagac agtaaagtga ccgatcacca tgcacttttg ccaactgaaa acagagcacg
    19981 ttatgaaaaa ttaagtaatg aagagcgaaa aatctatcaa atgattgtct ctcgtttttt
    20041 aggactgttt gctcaacccc ataaagtcag ccaaacaaaa gttacggttg aatttggtaa
    20101 agaacgattc atttttcggc aaaatcgtgt cgtccaagca ggatggaaag gcgaaactga
    20161 aactgagacg gaaacagtca aatgggaaaa aggaatgaga atcaatcccg atttcgccat
    20221 caaaaaagaa ctatctgcac cgcctaaacc attgtcagaa gcaagcttgc ttggtcaaat
    20281 ggaaaaatac agtctgggaa ccccagctac gcgtgcagag attatcgaga aactaatcaa
    20341 atctgaactg atggaacgta cccctcaagg actgcaagta tctcctaaag gtaagcaatt
    20401 attagtattg gtaaatccct ctttagtaac accagatttg acagaaagct gggaaaaaga
    20461 ccttgaagct attgctgcag gaaagaaaca agcaggaacc ttcttgaatg gaatcgaaaa
    20521 agaaacaaaa cgcttggtga atgagatcaa atcaagcaaa caagaatatc aagatttctc
    20581 gattacccaa aagaaatgcc cagaatgtgg ggccaatctg cgcgagaaaa acacacgtga
    20641 cggcaaaatc tatgtctgca ccaattcaga ttgcaactac cgcagaagaa aagatccaaa
    20701 agtctctaat catcgatgcc cgcaatgtca ccggaaaatg gaaatcatcg aaggtaaaaa
    20761 cggtgcttat ttccgttgta aatacgatgg aacgacagaa aaattgcttg ataaaaaaga
    20821 gcagaagaag aaaatgacca aacatgaaga acgacgcttg ctgaaaaaat atgcacaagc
    20881 agaagaacca gaagaaagcc cattagcagc agcactaaaa gcagcaatga agaagtaata
    20941 agatatgaaa gaggttgtga tagaagtggt cagcttcaag aaataagaag gaatttacga
    21001 aaattgcttc tcaaattttt gtgaaatttc ggcttatttc cgaagaagct acttctggaa
    21061 caccatttat tcggaaaaaa ggactgtgac aagacttttg tcacagtcct ttttcattta
    21121 aaaaaatgtt tacttcattt gacgtatatt attggtgagg cttcaattca tttggcttca
    21181 ctttttctgt ttctcttatg atataaacag gacgctgttt cgtttctaag aagattttcc
    21241 caatatattt cccgatgatt ccaagacata acaattggat tccaccaatg aacaggaaga
    21301 tcgagaccat ggaaggccat cctgatgtag gatcaccaaa caacaatgcc cgcaaaataa
    21361 tgaacaaaat tgcaatacca gaaccgacac aagacaaagc gccgataaat gaagcaatcg
    21421 ttaacggtgc atccgagaag ttcacgatac catcaatcga gtagttgaat aatttccaga
    21481 aggaccaaga tgtttcgcca gccacacgtt cgcggttttc ataagaaata tattctgttt
    21541 taaatccgac ccagctaaat aatccttttg aaaatcggtt gtattctgtt aattctagga
    21601 cggcatccac catttgacga gtcatcaagc ggaaatcacg agcgccatcg accatttctg
    21661 tatcgctgat tttattgatc aactggtaga attttttcgc aaagaaactg cgtatcgcag
    21721 gctcgccttc acgagtcgaa cgccgggtgc cgacacaatc gatatcagaa gtagtgatcc
    21781 ggttgatcat ctcaggaagc agttcgggcg gatcttgcag atcaacatcc atcacggtca
    21841 ctaaatctcc tgtagaagct tgcaatccag cgtacagagc cgcttctttc ccaaaatttc
    21901 tggaaaatga gacaaagcgg acataaggat aagctgaggc tagcttcttg attgctgcta
    21961 acgtaccgtc ttttgatccg tcgtcaataa acaaatattc gaactcatgg gcacctttga
    22021 atttttcaac ctcatcaaaa aaaagaggaa cagttttttc ttcattaaaa catgggacaa
    22081 caattgatac tttcatcgtt tctatccttt caatcgtatg tttatatatg gtttacattc
    22141 aaaatttaat aaaccttaaa taacaccttt aaaatttgat tttacgtaaa agaacaggta
    22201 ttctttttgt aaactaacgt atttagttta ccatgtttcc tcggtttatc aaacttttaa
    22261 gaacaatttt tattagttgg caagtagttt tggatagaga aaaaggagga agttccagtc
    22321 tattgactag gagcaaacaa aatgaaaata cacacaccta tcggcttttt atggttagat
    22381 gcagatgaat ccggattgac tgctgtatct tttcagccaa tttcagaaag cgaatcagcg
    22441 aatcagcgaa ttttaacgcg cacacaagaa cagtttgaag aatattttgc gggaaaaaga
    22501 aaagaatttg atgttccttt atctataaag agaggtacgc cttttcagca aaatgtctgg
    22561 aaagctttgt cgaccattcc ttatggagaa atccgaacgt ataaagaagt agccgttgct
    22621 gttgatagtc cgaaagctgt gcgagcgatt gggcaggcaa atcgaaccaa tccattgcca
    22681 atcgttattc cttgtcatcg agtgatcggg caaaatggaa aactgacagg atatatggga
    22741 aacgcaatag atggggttga aatcaaacgc caattgttgt tgttagaagg atatctgcaa
    22801 aattaaaaac agttgtgatt cggacattgt tccatgcgag aatcacaact gttttttcat
    22861 gttcattcta aactttgatc agccaaataa atagtaaagc catcactgtt tggagaagcc
    22921 cgacagctaa tccataagat aagaagcgtg taccttcttt catgaatttt gcaaagtcta
    22981 aacgcagacc aattgccgct aaagcagtga tctcaaacca gctgctgata aaatgtgccg
    23041 attggtcaat aactacaggt aaagaaaaca agctgttgac caaacaaaag atcacaaacc
    23101 ctaatacata ccaaggaagc ggtaattttc gttttttctg cgtatcagtt ggcgtttcaa
    23161 acagagctgg ttgtttcttg tgcatgaatt tttcaaacag atagacaaca acaacgagca
    23221 tcataatacg tagaatctta aatagcatgg cgaattcgac aacagaattg tcaagtaagc
    23281 ttgccccagc gactacttga ccaacagatt ggagtgtacc acccaaaagt gcactttttt
    23341 caatgagatt tccaccaaac aactgtaatc caagaaaagg gagagtcaac atcatcacag
    23401 tacccagtag attgactagt gtgatgatct gtcctttttc ttcctctttc gcatggatgg
    23461 aaggggcgat tgaagcaata gcagaagaac cacaaacagc gtttcctcca gccatcatca
    23521 aagaagaagt atcgctgaat tgcagttttt taccaatcaa gtaagcaccg atgatagtga
    23581 ctgacatttg cagtaacaca aaaacaattc cttttacacc aatttgagca atcgtttgag
    23641 ctgtgacagt tgcacctagt aaaaaaacag agaattctaa tagtttgctt tcagcaaatt
    23701 ttgtgccgat tgctagaaca ggctgtttaa aaaaagtatt tcctaataaa atacccaata
    23761 aaatagcaat agtcgctcca cctaactgtg gcagataaat agcaagaagc ctgctgaagg
    23821 cagcgatggc aaaagaaagc agtaaaccgg gtaagatttc gcggaccgct gaatccttta
    23881 cagattttat tggatgtgac aatgatttca tttgatcggt tcctttcgtc aataaatact
    23941 aaaagcatac tcgaacatag tagcactaaa ccagtatttt gacaaaagaa caagaggtcg
    24001 tgacaaaagt cttgtacgac ctcttgtttc gaataaacgg tgttccagaa gtagcttcta
    24061 cggaaataag ccgaaatttc acaaaaattt gagaagcaat tttcgtaaat tccttcttat
    24121 ttcttgaagc tgaccacttc ttttacgacc tcttggtaaa cggtgttcaa aagcttgcat
    24181 ctgcggtaat aagcccaacc agtacaaaaa catgaacagc tgtttttgtt tggccgttct
    24241 tattacttga tgcagaacag ctttttcaca acctattgaa tcgattatta agcgattttt
    24301 ttagaaacaa attttccatg tttgctatgt tctttaatta agaaacgtac gtaatgagca
    24361 agaacgtaga agacaactac acttgcaaac agacattccg caacttttaa gaaatccaat
    24421 acgctactat agatacctaa actgttgaag taatgaacag tagtaaatat agctgttgca
    24481 gagataacaa gcgggaatgt aaaggctgca tagcttggat aaaaacgaag cttgaacaaa
    24541 cgtggaagca tagacagcac aacgaaatac aatacttgag ataaagcgaa caatgctacg
    24601 acaagataaa aattaggttc agcaaagttt ttaagataac ctgttaggca aagagaacca
    24661 ggtgctgcaa cgatcgtaat cagcggacgt gtcggatgag ccatatttct tacgaaaaag
    24721 acacgtttga tgatgatcgg caataagaac gcatagcaga tcaatgaaat ccaaaagaaa
    24781 atttttccaa taagtgggaa gaagttcccg gcagttacag tgatgacccc aaatccaacg
    24841 taaatgataa accagcttgg ataaattgct tcaattgaaa gatcagcttt gataacatgg
    24901 tgataattga aataaaagac aaggatcact tggaagataa cagcgcacag ccaaatgaat
    24961 ttgacaattg gtgcaatact ttcatatgaa acaaaataag tacaaataac catgagtgac
    25021 atcgtaaaag ttggtgaaac ggaggcgatg attggatcat gcaacgtttg tttcgtgtgt
    25081 tcgaataaga taaacaattt aacaaaaatg aatatcataa gaatcataga gataccgcca
    25141 gttagattgc ctaaagcaac atggtgatag tctttaaaca gatttccaag ggaagctaac
    25201 ccaaggatca acccgcagat cgggatcgga ataattttca aaaagttctt catatataat
    25261 attctccttt ttatttaata gattttagta ccaagatgaa cgaatacatt gtaaaatgaa
    25321 taggaatgta tgattacgag caagcagaca gctgattgtc ttcatctaag ttactagttg
    25381 gatcagcaac tgttttgttg tacatttcta ttatagatgg ttttttgtat atttaaatca
    25441 aatagttttt atagtaatta tcataaaaag ttatggaagt ggtgccatgt ttaagttatt
    25501 aacgactttt cgagcagtat atgaaacaag aaatttttcc agagcagcag aagtactgtt
    25561 tttatctcaa ccagcagtct cgaatcaaat caaacagttg gagcgggaat tgaatatcga
    25621 tctttttcaa cgtaacggaa gaaaagagat gcttccaact aagcaagccg acttattata
    25681 tgaacggaca ctggttttgt tagaagaatg ggaagaaact cagcaaaaat tgctcaatga
    25741 acaaaatgaa gaagaagtat gtcggatcgt tgcttcccac acctttgctg tctatctttt
    25801 gcctcagttg atgaaacatc tgatcaaaag gtttcctaaa gtgcattttg agatcgaaat
    25861 cacgaattct catgaatcat tagaacaagt agcgaaacat gaatccgatt ttggttttat
    25921 cgaaaagcca ttagagactt ccggcgtaca aagatactct ttgatggaag accagctagt
    25981 catcgctggg gacccaaaaa gcaaattgtg gcttgtaaga gaagcaaact ctggtgtttt
    26041 tcattacacg cagcgttatt ttgaagagat gaatatccaa gataagaaga tgatcgtgaa
    26101 aagtaacgat gtaattgttg cgttgctgaa ggaaggaatc ggtcgctcga ttttgtctaa
    26161 gcgctctgtt ccagaaaatg tgccaatgac tccgttgaat ttccatcgat atttttattt
    26221 catccaacga accaatctgg tttctgatca gctgctagag attgccgaag agatccttcg
    26281 atacaatgaa agcacagaaa cttctcacag cgaggagaat ctcagctggt agataattgc
    26341 atgtatcaaa aaaagttcta tgatacacta aagataagca gtaggctttt gtactgcatt
    26401 tgctcaagca actgcacaaa cggaaagtta atttttccca aatgtctatg gaaagacttt
    26461 ccattatcta actggaccgc tataataatc agcagtatgg tttttaagaa aagggatgaa
    26521 acatggaaaa aaaattaaaa gcttcgaacg ataatcgtat tgattatggc gtgattttac
    26581 cagtctttct gttatgtttg attggtctgc tctcattata tgtagcactt agccatgatc
    26641 cgaacaatcc agcagtcttg aaaggcgtag ggatgcaagt tgtctggtat ttagttggag
    26701 cggcagcaat cgttgtcatc atgcatttga attccaaatg gatctggaaa ctgactcctt
    26761 acctttatgg tttggggtta gtggtaatgg gactcttgct caaattctat gatcagaatt
    26821 tagctgcttc aacagggtct aaaaactggt ttcgattcgg tgcgtttact ttccagccag
    26881 cagagttgat gaagattgcg tacatcttga tgatggcact gatcgtaact aaacataata
    26941 cgcaagtaaa agaacggacg atgaaatcgg acttttggct gattgggaag ctcttgatcg
    27001 tgacaatacc ggttttagca ttgatcatgg cccaagatga ctttggaacg atgcttgtct
    27061 ttcttgcgat ctttggcggg attttcctga tgtctggaat ctcttggcgc atcatcgcac
    27121 ctgtagtaat cttggcagta gtcgttggag caggaacgat cttcttggtt acgactgaag
    27181 gcggaagaga cttgctttat aaagttggat tcaaatctta tcagtttgca cgtattgatt
    27241 catggctgga ccctttccat gatacgtcag gaatgagtta tcagccggcc caaggacttt
    27301 tggcaattgg taccggtggc ttgtttggta aaggattcaa cgtaagcaat atctatgtgc
    27361 ctgttcgtga gtccgatatg atttttaccg ttatcggtga aaacttcggc tttatcggtg
    27421 gtgcattcgt cattttcttg tattttattt taatctatcg tatgattcgg gtgtgtttcg
    27481 atacgaataa tgaattttac gcctatatcg caagcggttt gatcatgatg ctgctgttcc
    27541 atgtctttga gaatattgga gcaaatatcg gattacttcc tttaacaggg atcccattgc
    27601 ctttcattag tcagggagga tcctcgattt tagggaacat gatcggtatc gggttgatat
    27661 tatcgatgag ataccaaaat gaagcacctg caagatcttc aaggaggtaa gaaaattgta
    27721 caccttttac tggtacccga aatgttcaac atgcaaaaaa gccaaagcgt ggttagacca
    27781 gcatggcgtt agttatcaaa ccgttgatat gatcaaagaa gtaccgaagg cctcaacgat
    27841 tgagacttgg ctagaaacga gtcaagtacc aatccgtcgc ctgttcaaca caagcgggat
    27901 gaaatatcgg gaacttggac tgaaagatca agtaggcgag tttacggctt caaaagcgag
    27961 ccaagtctta tcgacagatg gtatgctgat caagcgacca ctgatcttac gagaggacca
    28021 gcttgttgca attggattca atgaaaaaat ttatgaagga gaactgatat aaaatggcag
    28081 gcaagcattt gaagaaaaag gacaaccttt ggatcttatt taatggaaaa gaatactgtg
    28141 ttggtttgac aaatgaagcg caagaagaac ttggagacat cacgtttgcc aatctgccaa
    28201 aagcaggaca atcgctaaaa aaagatgaac ctctgatcga agttgaagcg gaaaaagcgg
    28261 tcaatgaatt tgccagcccg ctaacgggta cagtttcttc tgttaatgat aaaatcggtg
    28321 aagatatcaa tatcttgaat gattcagatg aaatgaatgc gtggatctta agtttcaaag
    28381 atgttgatcc aaaagaattt gacgaattat aaaatcagaa aatcacttga aaaaagttga
    28441 cactgattct tccagttgct ataatcataa gcaatcctat atgaaacgag aaagctttga
    28501 aaagaagagt acatgtcacg gacgtttgaa gagagccctg tttgctgaaa atgggtaacg
    28561 aaaatgagat ggaagatggt cttagagcag agtaagcgag cgtaagcaaa cttactacgg
    28621 gagtgcccgt tacagcgcga cagtatacgg aaagaagcta agtccaagta ctggaagagg
    28681 ctgttattgc gaagtaacgg taaatcaagg tggtaacacg gaagttcaag ctttcgtcct
    28741 tttgcttaag gcaagagggc gaaggctttt ttgtttgaaa aattataaga gaaagatgtg
    28801 aatggcatgt cattaatcca actaacgaat gtcaaaaaga cattcaatgg gaaaaacgga
    28861 aacatccaag ccgtcaatga cgtttcacta aacgttgaaa aaggggatat ttatggaatc
    28921 gtcggctatt ctggcgcagg aaaaagtaca ttagtccgtc tcctgaatgg acttgagctt
    28981 cccacgagcg gcgaagtgat cgtaaacgat caagatatta caaaattaaa aaacaaagaa
    29041 ttacgtacat tccgaaagaa aatcggtatg atcttccagc atttcaacct gttatggtca
    29101 agaactgtac ttgaaaatat ccagctgccg ctggaattag ctggcgtacc aaagagcaaa
    29161 cgtaaagaac gggcagaaga actgttgcgt ttagtcgggt tagaaggacg cggacaagca
    29221 tatccttcac aactttccgg cggacagaaa caacgcgtcg gtatcgcacg agcattggca
    29281 aatgatcctg agatcctgct ttgtgatgag gctactagcg cattggaccc gcaaacaaca
    29341 gaagaagtat tagacttatt gttagcgatc aataagaaat tgaatttgac catcgtcttg
    29401 atcacacatg aaatgaatgt gatccgcaaa atctgcaaca aagtcgctgt gatggagttg
    29461 gggaaagtcg tagaagaagg agacgtattg actgtcttca gacaaccgaa aaaagaagta
    29521 accaaacgct ttgtacaaca agacatcgaa ccagaagctg attcgacaga actattagct
    29581 gaactcatca aagaaaatcc aagcggccgt ttggcaactt tgacctttag cgaaagaaat
    29641 gccaacgagc cagtcatttc ccaagcgatt cgacgctata acatcgatgt caacgttgtg
    29701 tatggaaaga tcaaacaagt gaaagaaggt tcctttggtt cattgactgt cttgatgact
    29761 ggtgaaacca aagaattaga tcaagcagaa gtgttcatca aagaacaagg agtaggaatt
    29821 gaggtgatcc atcgtggata aaagcttttc agaaacatat ctagatttta aacaaatcaa
    29881 cccggaaacg atgaatcagg ctatccttga tacactatat atgacattaa tctcaatggt
    29941 cttagtgacg atcatcggat tagttttggg attgatcctt tactctattg gacgcaaaaa
    30001 caaaccgtat gctcgaatca tttatggaat cgtatcgatc atcagtaata ttttccggtc
    30061 aacgccattt atgatcctga tggttttgat cattccattt actaaagcaa tcgtaggcac
    30121 tatgctaggt gcgcaagcag ccattccagc tcttgtttta tccgcagcac cattttatgc
    30181 ccgtctagta gagattgctt ttcgtgaagt cagccctggt gtactggaag cagcagatgc
    30241 aatgggagca agttactggg aaatcatctg gaaggtcttg atacctgaaa gtattcccgc
    30301 attgatttct ggattaacag ttacaacgat ctcgatgatc ggatttactg cgatggccgg
    30361 cgcgatcggc gcaggtggac ttggaggtct tgcttggcag gaaggctacc agcgtggtaa
    30421 tctaacagtg acattcgttg cgactttaat tattttacta atcgtattta tcgttcaagg
    30481 aatcggtgat ttcttaacta agaaaacaga caaaagataa taggatatag ggggatgtag
    30541 gatatgaaaa agaaaatttt tggatttgca gcagcgcttt tgctgactgt aggattagca
    30601 gcatgcggta acaacagcga cagcaaagat tcagcaaata aagcagacga cacgacattg
    30661 aaagtcggcg cttcaccgac accgcacgca gagattttgg aacatgtgaa accattattg
    30721 aaagatgaag gaatcgatct tgaaatcgtc aaattcgatg actatgtatt accgaaccaa
    30781 tctttggaag aaggcgacat tgatgccaat tacttccagc atattcctta tttgaacaaa
    30841 gaaatcaaag aaaaaggata tgactttgtc aatgccggtg ctgtccacat cgaaccaatg
    30901 ggcttgtact ctaaaaaagt caaagatgtt tccgaattaa aagatggtgc aacaatcatt
    30961 acttcaaatt ctgaatcaga ctggggacgc atcatcacaa ttctacaaga tgctggctta
    31021 gtcaaagtca aagatggagt agatctggaa acagcaacat tcgatgatat tgaagaaaac
    31081 cctaagaact tgaagttcaa ccatacgatc gacccatcac ttttagcttc tacgtaccaa
    31141 aatgatgaag gtgacctagt tgcaatcaac gcaaacttcg cttacggcgc agggttgaat
    31201 ccattagaag atgccgtatt gcttgaaaag gacaactctc cttacgtgaa tattattgcg
    31261 actcgtaaag gtgatgaaga cagtgacaaa atcaaaaaac tgattgaagt gcttcaccgc
    31321 aaagaagtcc aagaatggat cacagacaaa tggggcggct ctgttaaacc agtaaaggct
    31381 gatgcaaaat aaggtatgga acaaaaaaga tgagaaataa gtctgtaatg gcttatttct
    31441 catctttttt atacctattc taatttagaa atcaaaattg tttaataata aataaaagat
    31501 atttgatttt agtaaggaag gtcattgcgc ttcattctca attagtctaa aatgagtgag
    31561 aacgaaagag aatttggggg tataatgcat gtctgtctta gaaataaaaa atctacatgt
    31621 gtcgatcgag gaaaaagaaa tccttaaagg cgtaaaccta acaatgaaaa ccggagaaat
    31681 ccatgccatc atgggaccta acggcacagg aaaatctacc ttatctgccg ctatcatggg
    31741 caatccaaac tatgaagtaa cagaaggcga aattttgttt gacgggaaaa atgttttaga
    31801 cttggaagta gatgaacgtg cgcgacttgg cttattttta gctatgcaat atccaagtga
    31861 gataccaggt atcacgaatg cggaattcat gcgtgcagcg atcaatgcga aacgtgatga
    31921 aaacgacaaa atttctgtta tggcattttt gaaaaagtta gatgaaaaaa tggctttact
    31981 aaatatgcca gaagaaatgg ctgaacgtta tctgaacgaa ggtttttctg gcggagaaaa
    32041 gaaacggaat gagatcctgc aattgttgat gttagaacct acatttgcaa ttttagacga
    32101 aatcgattca ggtctggata tcgatgcttt gaaagtcgtt gctaaaggag taaatgaaat
    32161 gcgcggagac aatttcggtg cattgatcat cacccactat caacgattat tgaactatat
    32221 cacacctgat gtcgttcaca tcatgatgga aggccgagta gtcatgacag ggaatgctga
    32281 cctagctaaa cgtctggaag cagaaggcta cgctggtatc agtaaagaat taggaatcga
    32341 ctataaagaa gaagaagcgt aagggggagc aagaatgaaa gaaaaaaact ggctcgatta
    32401 tcttgacgcg gtaaatgatt tttctttaag taaaggcgaa cctgattgga tgagaacctt
    32461 ccgtcaagac gcactagcga aagcagatga actgccattg ccgcatatcg atcgggtgaa
    32521 attccatcgc tggtcgttat ttgatgtgaa agaaacacaa accatcagtg aaacaggcac
    32581 gattccggca tttgatgcaa tgaaagacaa tcctgtgctt gtccaacaag gttcatggac
    32641 gatttttgaa caactgcctg tggaattagc agaaaaagga gtcattttta ccgacttgtt
    32701 cactgcaatg atcgaatatc cagaactagt ccaagaatat tatatgaaaa aagcagtaaa
    32761 catgaacgaa gaccaattga ctgcactcca tgtagctttt atgaacagcg gtattttcct
    32821 gtatgtgcct aaaaatgtcg tgatcgatga accactcgag tccttattca ttcaagatgg
    32881 cgcatccgat gagcacttct tcaagcatgt gttgatcgtg gcagatgaac atagtgaatt
    32941 ttcttatctg gaacgtttcc agacaaccaa agaacaagta gcaaaatcat caggtaatat
    33001 catcgtggaa gtcatcgcta aagctggttc aaaaatcaaa tattcagccg tagatcaatt
    33061 aggagaaaat attacttctt atatgaatcg ccgtgggcac attttacgag atgcttctgt
    33121 cgactgggca attggtgtca tgaatgacgg tcatgtgatc gctgactttg actcagattt
    33181 agcaggagaa ggtgcacacg cagaagtcaa aatcgtggca atcagctcgg gcagacaaat
    33241 ccaaggaatc gatacacgag taaccaataa agcccctcat acgatcgggc atattttgca
    33301 acacggagtg atccgagaaa aaggaacatt gacctttaat ggtatcggcc acatcttgaa
    33361 aggggcaaaa ggtgcagatg cccaacaaga aagtcgtgtc ttgatgcttt ctgataaagc
    33421 acgtggtgat gcgaatccta ttcttttgat cgatgaaaac gaagtaactg ctggacatgc
    33481 tgccagtgta ggacgagttg atccagaaga aatgtactat ctgatgagta gaggattgca
    33541 taaagaagaa gcagagcgct tagtgattcg aggattcctc ggttctgtct taacagcaat
    33601 ccctgtagaa caagttcgga aagaattagt cgaagtaatc gaagggaagt tgaatggatg
    33661 atcgatgcta aaaagctacg caatgatttt cctatcttat ttcaagacgt caatgatgaa
    33721 cctttagttt atctggataa tgcagcgacg acccaaaagc caaaacaagt actccaggca
    33781 ttagcgtact attacgaaca cgataacgcc aatgttcatc gcggtgtcca tacgttagca
    33841 gaacgcgcga cccatgatta tgaacaagca agagaaaaag tacgggcgtt catccatgca
    33901 aaagaaacag cagaagtgct gtttactcgt ggtacgacga caagcttgaa ttgggtagcc
    33961 agaaccttcg gcgaccgcta tatcaaagca ggagacgaga tagtgctctc ttacatggaa
    34021 catcattcta atgttatccc gtggcagcag ttggcaaaac gaacaggtgc tgtcttgaaa
    34081 tatatcgaac taacagaaga aggcttcctg gatatggagg atgcagcgaa aaagattacg
    34141 gacaaaacca aaatcgtctc cattgctcat gtatccaatg tcttaggtgt cttcaatcct
    34201 gttcaagaat tagctgaact tgtccatgaa aacggcgggg tcatcgtagt agatggtgct
    34261 caatctgccc cgcacatggc cattgatgtc caagcactag actgtgattt ctttgctttc
    34321 agcggacaca aaatgtgcgg accaacaggt attggtgttt tatatggtaa acgaaaatgg
    34381 ttagaagaaa tggaacctgt tgaatttggt ggcgaaatga tcgattttgt cgaattatac
    34441 gacagtacat ggaaagaact accttggaaa ttcgaagcag ggacaccaaa tatcgccggc
    34501 gctatcgcat taggccatgc aatcgattat ttgcaaaaac tcggtatgga caatatccat
    34561 cgatatgaag aagaactagc cgcttatgtg ttgccaaaat tacaagcaat tgatggattg
    34621 actgtttatg gtccacaaga cccgaatttt cggacaggag tcgttgcatt caatttggat
    34681 gggttacatc cccatgatgt cgcgacagct ttagacatgg aaggggtagc cgtcagagca
    34741 ggtcatcatt gtgcgcaacc gctattgaag taccttgaag tatcagcgac tgctcgcgcg
    34801 agcttttatt tctacaacac aacgcaagac gccgaccgtt tagttgaagc aatcatggca
    34861 acaaaggagt ttttccaaca tgggactatc taaattagac aatttatacc gtcaagtgat
    34921 cttggatcat tcttcccatc cgcatcatcg tggaagcttg actgactcga caagcaagat
    34981 cgaattgaac aaccccacat gtggagatgt gatcgaattg caagtcgatg taaaagacgg
    35041 aatcatcaaa aatatcgcct ttgacggcag tggctgctcc atcagtacgg caagtgcctc
    35101 gatgatgaca gacgccgtca tcggaaaatc aatagaagaa gcagaacatt taactgtaga
    35161 ctttctacag cttgtccaag gaaaagaagt caaagatccc gaccaattag gcgatgctgc
    35221 tatgcttggt ggtgtagcta aatttcccgc tcggatcaaa tgtgcgacac ttgcctggaa
    35281 agcactagga aaagcaatgg aaaacgatgg taccgcagct ttccatcatt accacaagga
    35341 agaggagtga gaacatgggc gtaccagaat tagaagaata taaattcggg tttcacgatg
    35401 atgtcgaacc ggttttcagt acaggagaag gcttgacaga agaagtgatc cgtgagatgt
    35461 caagaatcaa aggcgaacca gaatggatgc ttgaattccg tctgaaatct ctagaaacat
    35521 ttaacaaaat gccaatgcaa acatggggcc ccgacctttc agacatcgat ttcgatgcca
    35581 tcaaatatta ccaaaaacca agtgacaaac ccgctcgtga ctgggaagac gtaccagaaa
    35641 aaatcaaaga tacatttgaa aagattggta taccagaagc tgaacgagct tatctagccg
    35701 gtgcttccgc acaatatgaa tcagaagtcg tgtaccataa tatgaaagat gaattcgaaa
    35761 agttaggaat cgtctttact gacactgact ctgctttgaa agaataccct gaattattca
    35821 aagagtactt ttcaaaactt gtaccgccaa ccgacaacaa attagcagcg ctgaattcag
    35881 ccgtatggtc aggtgggacc tttatttacg tgccaaaagg cgtcaaagtc gatgttccat
    35941 tacaaacgta cttccggatc aatgcggaaa acacggggca attcgaacgt acgctgatca
    36001 ttgttgacga aggagcaagc atccactatg tcgaaggttg tacggcacct acttattcaa
    36061 gtaacagcct gcatgccgcc atcgtagaaa tctttacaag aaaagatgcg tattgccgtt
    36121 acacaacgat ccaaaactgg tcagacaatg tgtacaactt agtaaccaaa cgagcaaaag
    36181 catacgaagg cgcaaccgtt gaatggatcg acggaaacct aggtgcaaaa acaacgatga
    36241 aatacccaag tgtttactta gatggaaaag gtgcacgagg caccatgctc tccatcgcct
    36301 ttgcaggagc aaaccaaatc caagatacgg gtgcaaaaat gatccacaat gcaccaaata
    36361 catctagctc tatcgtctct aaatcaattt caaaagacgg cggagaagta aactatcgag
    36421 gacaagtaac ctttgccaaa aacagtgcgg gttcgatctc tcatatcgaa tgtgacacga
    36481 tcatcatgga cgaatggtcg aaatccgata cgatcccatt caatgagatc cacaacagcc
    36541 aagtctcttt agaacacgaa gccaaagtct caaaaatctc agaagaacaa ctctactatc
    36601 tgatgagccg aggcctgacc gaatctgaag caactgaaat gatcgtcatg ggctttgttg
    36661 aaccattcac caaagaactt ccaatggaat atgcagtcga actcaatcga ttgatcagtt
    36721 atgagatgga aggcagtgtt gggtaaacct gttataggag ctagaaccct tgatata
//
LOCUS       DAZKLM010000018        36198 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3764
            SAMEA115706913-rid22582563.denovo.018, whole genome shotgun
            sequence.
ACCESSION   DAZKLM010000018 DAZKLM010000000
VERSION     DAZKLM010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706913
            Sequence Read Archive: ERR13196647
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 36198)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36198)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287823.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287823.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,004
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,849
            CDSs (with protein)               :: 2,849
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 41 of 96
            Pseudo Genes (incomplete)         :: 52 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 15 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36198
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706913-rid22582563.denovo.018"
                     /isolate="VRE3764"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..288
                     /locus_tag="ACG842_000827"
     CDS             <1..288
                     /locus_tag="ACG842_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0247389.1"
                     /translation="LKSFLKVISKKRNLIKTTQLLKKYPGLLHTSIQFPQLITMIHRF
                     IRMQQLPSKKLFLPLTDTFRVVNREDKKKVIVIDFTRNRNVLHQVAMEKAN"
     gene            347..787
                     /locus_tag="ACG842_000828"
     CDS             347..787
                     /locus_tag="ACG842_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0247390.1"
                     /translation="MMQTWLERLIRFQQNYHKLMQGRYAKFDQLNRGLLIAALVFSLL
                     SRWLPYRIGQLLFIVFFGWMIFRFLSKKIYPRLNENQRYLKYLDRVKEKYHQIKNKKQ
                     VKMDRKSYTFFECPNCHQKQRAPKGKGRIRVTCKTCGIKFETNV"
     gene            799..1467
                     /locus_tag="ACG842_000829"
     CDS             799..1467
                     /locus_tag="ACG842_000829"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HFQ0247391.1"
                     /translation="MKLVLLRHGESEANFENYWTGWLDVALTEKGKEQAAVAGQKMLE
                     AGLQFDAVYTSVLKRAILTGQVALEEMGQLWLPIIKTWRLNERHYGDLVGKNKDKMKE
                     IYGKDQVKTWRRGYTEVPPLTAENRFDRRYDHLDPHLIPYGESLEMTVNRIIPLWQDH
                     LAPKLKDRQDLLVVGHGNSIRALTKYLEDVPEDQMDTIDIPNAQPIQYTLADDLTILD
                     KKIL"
     gene            1531..2154
                     /locus_tag="ACG842_000830"
     CDS             1531..2154
                     /locus_tag="ACG842_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HFQ0247392.1"
                     /translation="MKNYIWDFDGTLFDTYPAMVDGAWQALKDFGISMDKKEIYFKMK
                     KYSTSYLINESNLNAREFNELFHRYEKESTEVSRPFPETKQVLEMLKDNGGRHFILTH
                     RLTESTWGLLKEHRLAHLIEEVVGIDQDFPRKPDPASLNYLIDTFHLERTDTMMIGDR
                     RLDIEAGKNAGVATCLYDIDHFLGEIPADYVVGNLNEILTLQSIIKK"
     gene            2210..2596
                     /locus_tag="ACG842_000831"
     CDS             2210..2596
                     /locus_tag="ACG842_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1149 family protein"
                     /protein_id="HFQ0247393.1"
                     /translation="MEIRRQKEVVEAFHYDMRLPNQEVETDLKVGFSPLKETIENYPK
                     EHSIVGARLEFRLVFDTYVLSGSVSQINHILNRKIEGQNDVTQEEVDELVAPLFDIVR
                     RMSYEVTEIALDQPGVKLNFQSEKQE"
     gene            2809..4200
                     /locus_tag="ACG842_000832"
     CDS             2809..4200
                     /locus_tag="ACG842_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine transporter"
                     /protein_id="HFQ0247394.1"
                     /translation="MTTLITVLVVLVFAALLYVFYRMQKRHTKFSTRVFTALGVGIVF
                     GGLLQLIFGVDDKVVTQSMEWIGIVGQGYVSFLQMLVMPLVFISIIGAFTKMKVSEKL
                     GKISATVLTSLLGTTAIAALIGIGSTMLFGLQGAKFTQGAAETARISELATRQETVEN
                     LSIPQQILSFIPKNVFADLAGSRATSTIGVVIFAAFVGIAYLGVKRKAPKEAAFFSDL
                     VDSLYKIIMRIVTLVLRLTPYGVFALMTNVLATSDFAAIVNLGKFVVASYVALLAVLI
                     VHSLILIGAKVNPINYFKKAFPVLSFAFTSRSSAGALPLNIETQTKALGVDDATANFS
                     ASFGLSIGQNGCAGVYPAMLATIVAPAAGVNVFSLEFILMLVAIVTISSFGVAGVGGG
                     ATFASLIVLGAMNLPVAIVGLVISVEPLIDMARTAVNVSGSMVAGIVTSARIKDLDRD
                     VINDDSKVIDVNA"
     gene            4811..5179
                     /locus_tag="ACG842_000833"
     CDS             4811..5179
                     /locus_tag="ACG842_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HFQ0247395.1"
                     /translation="MFTNEIKIMLYVDDVEKNATFWRAVGFAEKDRQEMDGTLIIEVG
                     VEDSQTSFLLYDRAFVESHSPGTATSAPSLMFYSKDIFGLYKKMQTQSVTLGDMVQLG
                     EEYVFNFADPDGNYFAVTGK"
     gene            5359..6300
                     /locus_tag="ACG842_000834"
     CDS             5359..6300
                     /locus_tag="ACG842_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HFQ0247396.1"
                     /translation="MDYQVLLYYNYTKIEEPEEFAKNHLAFCKSLDLKGRILVAEEGI
                     NGTVSGTVENTTAYMKAMKADPRFADTMFKIDPSEGHAFKKMFVRYRPELVSLKLEED
                     IDPNQVTGAYLSPQEFKEAILDEQTVVIDARNDYEYDLGHFRGAVRPDIRSFRELPDW
                     IREHKEEFMEKRVVTYCTGGIRCEKFSGWLVREGFKDVGQLHGGIATYGKDPEVQGEL
                     WDGKMYVFDERIAVDINQVDKQIVGRDWFDGTPCERYINCSNPECNRQILASIENEEK
                     HLGACSIECAKHPRNRYVQDHGISPEEAELRFTALIK"
     gene            6369..7100
                     /locus_tag="ACG842_000835"
     CDS             6369..7100
                     /locus_tag="ACG842_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFQ0247397.1"
                     /translation="MKSLIYAIGDVHGKHELFEKLIHYYDPKIHQLVLLGDLNDRGPR
                     TKECFLLGKQLVEETGAIYLRGNHEEYFLQFLQSPEDWYAPYMRNGGKETFESLIHPG
                     ASEEYSPTEISMMIQSRYSELIKFLVQRPLYYEWENYLFVHAGVDLTKKDWRKTSLRD
                     FIWIREPFHEGKNNTGKTIVFGHTITPMLHGDMQTTDLWIQDHKIGMDGGAVFGGSLH
                     GVVFDKEGILQDIEYQNNEGPWHGI"
     gene            7244..9424
                     /locus_tag="ACG842_000836"
     CDS             7244..9424
                     /locus_tag="ACG842_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tex family protein"
                     /protein_id="HFQ0247398.1"
                     /translation="MTENLNQTIIQLVQKELSDYRPKQLTTVLNLLNEGNTVPFIARY
                     RKEMTGSLDEVQIREIEERYAYLENLEKRKTEVIRLIDEQGKLTPELEAEITQAVKMQ
                     QVEDLYRPYKQKRRTKATIAKEKGLEPLAKWLMQLTDGEVQSEAEKYIDKEKEVSSAE
                     EALHGAHEIIAEQVSDNAKFRTWIRSYTYNKGMYVSNVKDEQADEKGVYEMYYDFAEP
                     VHKMVSHRILATNRGEKEDILKVFFQVDEAAILAYLDRQIVKNPASPSSSFVREAYQD
                     SYKRFIQPAIERELRNELTEKADEQAIAIFGENLRNLLLQPPLKGKVVLGFDPAYRTG
                     CKLAVVDATGKVLAIEVIYPHKPAAQAKREAAGPAFIQLINKYQVDMVAIGNGTASRE
                     SELFVAEQLKSADHKTYYAIVNEAGASVYSASEIARKEFPHLQVEERSAVSIARRLQD
                     PLAELVKIDPKAVGVGQYQHDVSQKRLAEQLDFVVETAVNQVGVDVNTASPQLLQHIS
                     GLNKTTAQNIVIYREENGEFTARTQLKKVPRLGPKAYEQAIGFLRVPGGKNILDNTGI
                     HPESYSIAKEILTSVQLSEKELGTEEAVEKLTRLSVEKLAESLSVGEETLADILAGLT
                     QPGRDMRDEMPAPLLRTDVLSMEDLKPGMELTGTVRNVIDFGAFVDIGVKQDGLVHIS
                     KLSKKFVKHPTDVVSVGDIVTVWIEQVDTKKGRISLTMLSPYEE"
     gene            9444..9926
                     /locus_tag="ACG842_000837"
     CDS             9444..9926
                     /locus_tag="ACG842_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HFQ0247399.1"
                     /translation="MTQEELQQLVEEISLKSFQLPFKHQAMFNPRLRTTGGRYHLNDH
                     HLDFNAKLFEESDEETIAGIIKHELCHYHLHLAGRGYQHKDMDFKYLLKKTGGLRYAP
                     ALTKKQIKMEYYRCQDCSATICRKRRIDVRKYRCGRCHGEIKWIETKREDFNEAQKIR
                     "
     gene            9942..10703
                     /locus_tag="ACG842_000838"
     CDS             9942..10703
                     /locus_tag="ACG842_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HFQ0247400.1"
                     /translation="MEKSEQRNLKDWNQLLNFLAAPEEGNFEELTAPTLILAGNCLPI
                     LADKAAQMYLNGQVDQLFLVGGVGHATRILYENFEKQGFHFEDGMSESEICRQYLKEV
                     YDLPDKAFLIESKSTNSGENATFSLEILHSLGAVPEKILLMNDPTLQRRTRATFEKVW
                     QNEQTIFANAVPFVPEILHFSEEIIFTAKELNHQWPKEYFHALVLGEMERLHDDENGY
                     GPKGKDFIPHIDISEEVWSSYTRIKESIKTDFSRT"
     gene            10834..12168
                     /gene="murC"
                     /locus_tag="ACG842_000839"
     CDS             10834..12168
                     /gene="murC"
                     /locus_tag="ACG842_000839"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HFQ0247401.1"
                     /translation="MENQNKLYHFVGIKGSGMSSLALVLHEQGLNVQGSDIEKYFFTQ
                     RDLEKANITILPFNADNVKPGMTIIAGNAFPDSHEEIQRAKELGLEVIRYHDFIGHFI
                     QNYTSIAVTGSHGKTSTTGLLSHVLSGVRPTSYLIGDGTGHGDPQAEFFSFEACEYRR
                     HFLAYSPDYAIMTNIDFDHPDYYTSIDDVFTAFQTMAGQVKKAIFAYGDDAYLRKLKA
                     NVPIYYYGVTENDDIQARNIERTTSGSAFDVYHGDEFVGHFTVPAFGKHNILNALGVI
                     AVAYFEKLDLKEVAEEMLTFPGVKRRFSEKIVADMTVVDDYAHHPAEIKATIDGARQK
                     YPDKEIIAVFQPHTFTRTIALMDEFAEALDLADKVYLCDIFGSAREEQGNVKIEDLGA
                     KIKKGGEVIKENNVSPLLDYHDAVVIFMGAGDVQKFEQAYEKLLSSTTKNVL"
     gene            12475..12960
                     /locus_tag="ACG842_000840"
     CDS             12475..12960
                     /locus_tag="ACG842_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
                     /protein_id="HFQ0247402.1"
                     /translation="MNIGKPRKLGKTINEIEEGDSLSLTESIEDNQLLLYLGLTNDAN
                     PLYIQHDFAQKTEYQQPIVPSVMLMGIITSAISKHLPGPGSHVVNFSVNFIEPVFHYE
                     TLTFQFEVIKVDKMKDVVTISVEAENTNEDRVLDAVVMVQPPQVPELEIAIESEGELN
                     E"
     gene            12953..13636
                     /locus_tag="ACG842_000841"
     CDS             12953..13636
                     /locus_tag="ACG842_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HFQ0247403.1"
                     /translation="MNNPSTSMSGINRFYAKVYGFLGMGLALSAVTSYLALWVFPLQL
                     ATFINNFPLGFVGLWIVELILVMVLGVKAQKNPSLAIGGFIAYSLLNGITLAVTLAYY
                     DIGTVTQAFLTATLMFMGMSVVGVTTKRDLSGVGRAGQAALIGVIIALVLNIFILRSS
                     VVELFISLLLVVIFAGITAYDNQSIRNIYVQTNGTAHSGIAVFMALKLYLDFINLFLA
                     FLRIFGRNN"
     gene            13762..16407
                     /gene="polA"
                     /locus_tag="ACG842_000842"
     CDS             13762..16407
                     /gene="polA"
                     /locus_tag="ACG842_000842"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HFQ0247404.1"
                     /translation="MTKNKLLLVDGNSVAFRAFFALHNSLERFKNRNGLHTNAIYAFN
                     TMFENVMQKEQPTHVLVAFDAGKTTFRTEMYAEYKGGRSKTPGEFKEQMPYIRDLLTG
                     LGVQYYELPNYEADDIIGTLAEKVDKDQFDVVVLSGDRDLTQLASKEVKVDITVKGVS
                     DIESYTPEHVAEKYDGLTPKQIIDMKGLAGDASDNIPGVTKIGEKTAIKLLKQYGSVE
                     GIYEHIDEMKQSKMKENLINDKEQAFLSKKLATINTSAPVDVSIDSLKYEGKNLDKLV
                     PFYKEMDFNQFLSKLNIVEEEVKMDDILFEVVHEFKEEMFTTDMALYVEMMGDNYHTE
                     EIVGVAWGTEKKIYVTNDLSVFENRAFHSWITDPTRLKKVYDAKRTYVALNRYTGKTK
                     GIDFDVLLGAYLLDTNEKSTDIEGIAAHYGYNDIQSDEAVYGKGAKKGLPEEEELFFA
                     HLARKVAAINALTPKLSQELAEKNQEDLFRKMELPLSIILAEMEISGIKVDATRLQEM
                     KGEFSARLREIEQKIYEEAGEEFNLNSPKQLGVILFEKMGLPVIKKTKTGYSTAVDVL
                     EQLREQAPIVEDILTYRQIAKIQSTYVEGLLKVIQSDGKVHTRYVQTLTQTGRLSSVD
                     PNLQNIPIRLDEGRKIRQAFVPREKDWLIYSSDYSQIELRVLAHISNDEHLKAAFLEG
                     QDIHASTAMRVFGIEKAEDVTPNMRRQAKAVNFGIVYGISDYGLSQNLGISRKAAQQY
                     IDTYFEKYPGVKEYMERIVREAKDQGYVETLYHRRRYLPDINSRNYNIRSFAERTAIN
                     TPIQGSAADILKIAMIELDKRLKETGLQATMLLQVHDELVFEVPEKELESLDKLVKEV
                     MEQAVSLHVPLITDSSWGKTWYEAK"
     gene            16452..17288
                     /gene="mutM"
                     /locus_tag="ACG842_000843"
     CDS             16452..17288
                     /gene="mutM"
                     /locus_tag="ACG842_000843"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HFQ0247405.1"
                     /translation="MPELPEVETVRKGLERLVVGKTIQKVQVLWPKIIEQPETPIFEA
                     SLVGETIQSIGRRGKFLIFHLDHCELISHLRMEGKYQFTKENTPIDKHTHVLFFFEDG
                     SQLRYNDVRKFGRMTIVEKGASATYRGIMKLGPEPLPDSFLLADFANGLKKSHKAIKP
                     LLLDQRLVTGLGNIYVDEALWEAKIHPEQPANTLRPKEVEQLRLSIIDVLDRAIEAGG
                     TTIRSYLNALGESGGFQVALHVYQQTGKPCIRCGTPIVKTKVAQRGTHYCPNCQRLKI
                     AK"
     gene            17252..17881
                     /gene="coaE"
                     /locus_tag="ACG842_000844"
     CDS             17252..17881
                     /gene="coaE"
                     /locus_tag="ACG842_000844"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340744.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HFQ0247406.1"
                     /translation="MSELSKAKNRKVGFVLGLTGGIATGKSTAAKVFQSHGFPLIDGD
                     VIARETVEPGTLALRKIVSVFGQEVLQPDGQLDRGKLGMIVFPSKEKRQKLDQLLDPF
                     IRKAIKDQIASLSSNHPLVIVDIPLLYEGHYDHYMDAVAVVYTTPETQLQRLMKRNQL
                     TLEQAQQRISSQLPIEEKKQRADILFDNNGTKEKLVGQIENWLFANRFL"
     gene            17933..18265
                     /locus_tag="ACG842_000845"
     CDS             17933..18265
                     /locus_tag="ACG842_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HFQ0247407.1"
                     /translation="MGSLKNQKDKVVGNAKEKIGEMLNKENLIDEGRTQSETAEEKEK
                     QYQARIDEIEDQQAEEEAKRQDLTQERKNPTPDRSKTPLPESDHIPDEQKIEEQRMKH
                     YTGIEEKL"
     gene            18577..19068
                     /gene="nrdR"
                     /locus_tag="ACG842_000846"
     CDS             18577..19068
                     /gene="nrdR"
                     /locus_tag="ACG842_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HFQ0247408.1"
                     /translation="MRCPRCQHNNSRVIDSRQADDGRAIRRRRECENCSFRFTTFERI
                     EAAPLLVIKKNGEREEFNRDKILRGLIRSAEKRPVAMEQMEQIVNHVENRVRSLGENE
                     VSTNLIGEYVMEDLVDLDEIAYIRFASVYRQFKDMSVFLKELQDIVDKAKENNKENNK
                     ENN"
     gene            19091..20464
                     /locus_tag="ACG842_000847"
     CDS             19091..20464
                     /locus_tag="ACG842_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HFQ0247409.1"
                     /translation="MENAWKELQPKNIYQGRKAAPLREDSKNALLYLYQPLIGGEALS
                     IYFTLLSEISQESGKGPEGMHADLLSSLGCGIPQFYDARKKLEGIGLLDVYFKEDSDL
                     GPRFIYELLEPLIPKAFFKDSVLAFLLLEKVGERRFQKLTQFFEPKRHTAEGYQKITK
                     KFLDVYRFNEAVYAANQENIESLNKSFEESESVALLEADSTFDWQFLNDWLAKKHIAS
                     LDDPIIKQVDMYHQLYGFDELTLGELISQSYDFSEQKVSEKELQKAILAMQRRPVKQT
                     KETVAVSENSNEPSSNEHLTNAALQLIKEAQSIPPMKYLEAIKSEKGGYVSKQENWLL
                     QDLVSQSGLSSSVINVLLNYVLVIKNNASLNASYVNTVANEWAQKKIATAEEAIQHIR
                     QISNQAKQSKQKKQTNYQTGKRNVRREKLPDWVNQPKDEKQISSEKKAEIDRRFKEYL
                     AKKEGDI"
     gene            20464..21390
                     /gene="dnaI"
                     /locus_tag="ACG842_000848"
     CDS             20464..21390
                     /gene="dnaI"
                     /locus_tag="ACG842_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HFQ0247410.1"
                     /translation="MEDVGKELKRIISKRNYQEKYAEMIAEVLNDADVQAFLSAHQEE
                     LTQADIEKSYAKLYEFVQEKRKYQKNDPTMIAPGYEPKLTLNFHYVDVTYVPTEALLA
                     RQRQEEIRSRVKAMDMPKDIQSASFENFERTDGRGYASLEALDFVEKYTADPKGYHKG
                     LYLVGSFGIGKTYLLGATARELAIAGFTTTLVHFPTFAVEMKQAIGKDQVAEKLDAVK
                     RSPILMIDDIGADAMSSWIRDEVFGVILQYRMQEQLPTFFSSNFTMDELEQHLSVTQR
                     GDEEPLKAKRIMERIRYLTKEIEMTGRNRRNG"
     gene            21519..21959
                     /locus_tag="ACG842_000849"
     CDS             21519..21959
                     /locus_tag="ACG842_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFQ0247411.1"
                     /translation="MRAIFSSISVVGLFFLLAGCGSADSNTGSVDSIDAGQTVKTSST
                     KTERQSIFVGTIESITQVEAEEETLQLKLVDVMAKEDPANMGTSFSNDGVILNATAEK
                     ISVDINELKIGDKVQFTLVEMPIMTMSIPPQVPGDSIIDVSLNK"
     gene            22103..22804
                     /locus_tag="ACG842_000850"
     CDS             22103..22804
                     /locus_tag="ACG842_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine(22)-N(1))-methyltransferase TrmK"
                     /protein_id="HFQ0247412.1"
                     /translation="MNHLDLSQRLAAVGDFVPESARLADIGSDHAYLPVALILRGKIE
                     YAVAGEVVQGPYESARKQVKKDGLEDRITVRLANGLDAIQPQDGITAVTIAGMGGSLI
                     RDILEAGKLNQRLSGQERLILQPNIGERTLRSWLQENQYKIQSEKILEENKKIYEIIV
                     AEKEQKPVTYNEMELMFGPFLLEEKSEIFKSKWTRELQQRKAIREQLKQAANQEARQK
                     QIEQEIQMIEEVLKP"
     gene            22801..23922
                     /locus_tag="ACG842_000851"
     CDS             22801..23922
                     /locus_tag="ACG842_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051540 - metal cluster binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HFQ0247413.1"
                     /translation="MTISGRTFIQKFESYCPQWLAEEGDPVGLHIGTLDKPIQRVMMT
                     LDVRPEVVEEAIKKKIDLLIAKHPPIFRPVKRLVTDQPQEKMYADLLKHDIAVYAAHT
                     NMDIIWDGLNDWFCELLGIEVESYLVKTHEIHYKKLAVYVPVDHAQKMREVLAAAGAG
                     TQGDYTGTSFTSIGHGRFTPEAGAQPAIGKVGKTEQVQEAKVEVILPETIEKQVIQAM
                     RSAHPYEEPAYDLFAIDEPVEMFGLGRVGELPQEISIEAFVEQVKEAFQLDGLRIVQP
                     KNAKSSVKRIAICGGSGEKFYPQAIAQRADVYITGDIYYHTAHDMQSAGLIAIDPGHY
                     IESLCKQRFIEKFESWKQEENWDINFFVSETNTNPFQFN"
     gene            23937..25169
                     /gene="pepT"
                     /locus_tag="ACG842_000852"
     CDS             23937..25169
                     /gene="pepT"
                     /locus_tag="ACG842_000852"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0045148 - tripeptide aminopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HFQ0247414.1"
                     /translation="MYENLLPRFLRYVKTETRSDATSKTTPSTQTQVAFAQELKKELE
                     ELGLADVFYNEDNGFVMGTLPSNVDHEVRSIGFISHMDTADFNAVGVNPQIIENYDGE
                     STIVLDAEERYTLNTKDFPNLKNYEGHTLITTDGSTLLGADDKSGIAEIMTAMEILIN
                     NPSIPHGTIKVAFGPDEEIGVGADKFDVEQFDVDFAYTVDGGPLGELQYETFSAAQAN
                     ITIQGKNVHPGTAKDTMINALQLAIDFHNQLPADEVPEKTEGYEGFFHLMALDGNPEE
                     ASMSYIIRDHDREKFEARKAQITHIQEELNQRFDQERIKVDMYDQYYNMKEIIEKDMS
                     IVELAKQAMIELGISPVIEPVRGGTDGSKISYMGIPTPNLFAGGENMHGRFEFVSLQV
                     MEKATDVIVKIAELNTKS"
     gene            25301..26209
                     /locus_tag="ACG842_000853"
     CDS             25301..26209
                     /locus_tag="ACG842_000853"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HFQ0247415.1"
                     /translation="MEKLLVFYNKSSGKDEGEKLARWFQDYVKEKQPELYVELAETGP
                     SIDDAELVKKAEAIEADTLVIIGGDGTIHHIVQAFQKTLSQYHVGLLPGGTVNNLARV
                     LEIPLKKEEAADVILEGNVRKIDYGQVNDEVIISTLTIGILADTAVWISQEEKQRYGS
                     WIFIKKFFKLLLKKKKYHLDLETDEEHWKGKSQLLTVTMSNSVGGFTNFDDSATLDDG
                     KFHVTIVPSLDIFRFIFYLPRIMRGKFYTIPGITYFTAKRMKIQAQEKSVGTRTDGDT
                     TDDLPVELSVVSKGLTVYSPKKEEKA"
     gene            26244..26516
                     /locus_tag="ACG842_000854"
     CDS             26244..26516
                     /locus_tag="ACG842_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HFQ0247416.1"
                     /translation="MDVLLHNPLWFYLFIVNFYLFCLMGYDKHQARTSGWRVPENNLI
                     FVSLLGGGLGGLISRKVFHHKTRKKKFTIGFAAGVLVDLILIYFFH"
     gene            26527..27573
                     /locus_tag="ACG842_000855"
     CDS             26527..27573
                     /locus_tag="ACG842_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhN family protein"
                     /protein_id="HFQ0247417.1"
                     /translation="MSSKGTKTKIILNLLLLIVIFAIIYYLINQSFADIFAELLSTSV
                     VVLAAAVILGTVYQIAEGRSIKEIAEPFAPNFTTKDGFLTSSYIAFYRIVSFGTGTLI
                     SEIYFYNKKGMKLSQGAGVTALHMIMYKLAVITYALAGLVIQFSLFYSNGPSMIWFII
                     AGILLTIVIVAFLLAMSVSLNVQIFFVSLSNKLFKSERLRKWVDTCNQQIYSLRETVQ
                     TIIQDRTALLRIYLWNMLKLVFWYVLPYLILIENHPKIDFLLTISFISFAVVLSGVIP
                     TPAGIGSFEFVYLLLFRPVVGTVDAVSSLLLYRFSSFILPFLYGFVYVIAERRRVIKE
                     EIIEVKKENKENQG"
     gene            27824..30433
                     /gene="clpB"
                     /locus_tag="ACG842_000856"
     CDS             27824..30433
                     /gene="clpB"
                     /locus_tag="ACG842_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HFQ0247418.1"
                     /translation="MNIEKMTTTLQEAIAEAQQIAVTRKHQDIDIAHVWKIFLQSNHF
                     GRNFYTDAGLDVESFEHEIDRLLDEYPVVSGGNVQYGQNLSQNFFRLLNEADQIRESF
                     GDEFLSTEVVILALMKLKNYPLTVYLNKNGLSEKELRKNIEEMRGGERVTSQNQEEQY
                     KALEKYGVDLVQQVKSGKMDPIIGRDEEIRDVIRILSRKTKNNPVLIGEPGVGKTAIV
                     EGLAQRIVRKDVPENLKDKTIFSLDMGALIAGAKFRGEFEERLKAVLKEVKKSDGRIL
                     LFIDEIHNIVGAGKTEGSMDAGNLLKPMLARGELHLIGATTLDEYRQYMEKDKALERR
                     FQKVLVKEPTVEDTISILRGLKERFEIHHGVNIHDNALVAAATLSDRYITDRFLPDKA
                     IDLIDEASATIRVEMNSMPTELDQVTRRLMQLEIEEAALKKESDDASKKRLKNLQEEL
                     AELREEANAMKMQWETEKEEVNSVSAKRAEIDKAKHELEDAENNYDLERAAVLRHGTI
                     PQLEKELKELEAKAKDSEIKMVQESVTENEIAQVVGRLTGIPVTKLVEGEREKLIKLN
                     ETLHKRVIGQDEAVDAVSDAVIRSRAGLQDPNRPLGSFLFLGPTGVGKTELAKALAEN
                     LFDSEDHMVRIDMSEYMEKHAVSRLVGAPPGYVGYEEGGQLTEAVRRNPYTIVLLDEI
                     EKAHPDVFNILLQVLDDGRLTDSKGRVVDFKNTVLIMTSNIGSQLLLEGVTADGTIPE
                     AVAEQVNTLLRGNFKPEFLNRIDDTILFTPLSLDNVKGIVDKMVAQLAQRLEHQEILL
                     TISDEAKTWIAENAYEPAYGARPLKRFITKEVETPLAKEIVAGHVMPKSKVTITLLDG
                     QLHFKTEELEEIV"
     gene            complement(30584..31270)
                     /locus_tag="ACG842_000857"
     CDS             complement(30584..31270)
                     /locus_tag="ACG842_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0247419.1"
                     /translation="MEGLSVIKEIKKVQIALLAFGVFVVCYNFYEFITQKYSTSQGIT
                     FIVESLLGIALIFMPQVILKVFKLKIPAAIVLFYWFFLFISVFLGTGMHLISIISFWD
                     KILHAVSPMVLTALGYGLIGYLMKDAEISKTSPWLFLLFGFAFAGLCGVFWEFWEFLC
                     DQFLGMNLQRFAASDGTLFVGRAALMDTMGDLLTNTIGAALMGLFAWSQSKKDERYFE
                     SYKLEKVKNT"
     gene            complement(31248..31742)
                     /locus_tag="ACG842_000858"
     CDS             complement(31248..31742)
                     /locus_tag="ACG842_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside 2-deoxyribosyltransferase"
                     /protein_id="HFQ0247420.1"
                     /translation="MKIYFAAPMFAKSDLLYNQQLVKEIRELSSELSIYLPQENEAIN
                     DKTAYADSRMIALADTEKVLASDLMIALLDGLTIDAGVASEIGVAYAKGIPVIGLYTD
                     TRQQGGTHPKKIAALQETAENQFHYLNLYTIGLIKLNGKVVSSEIELLSEVKRFLDGG
                     TFSD"
     gene            complement(31762..32982)
                     /locus_tag="ACG842_000859"
     CDS             complement(31762..32982)
                     /locus_tag="ACG842_000859"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0247421.1"
                     /translation="MDQKDFRENLELKPVTQEHIDQFNELLSYVFQVTEADIEESGFE
                     NKRAFIRSKKPILEVSKVFGWFHENKLISQIAIYPCEVNIHGARYKMGGVTGVGTYPE
                     YANHGLMQDLIIVALDNMRKNKQWISYLYPYSIPYYRRKGWEIMSDKLSFKIRDTQLP
                     KQIPVSGMVERLPVDHPDVFSVYDKFAQQNHGALFRSDFHWEEYWRFENEDERTAAVY
                     YDSNHEPRGVLFYWVAEEIFHVKEMFYLDQEARNGLWNFISAHFSMIYWVHGDIYKNE
                     PLAFLIEDSQIKEQIEPYFMARIVDVKEFLQRFPFVGTADAFHFIIEDPVAPWNNGVF
                     ALTWDEQGQVRVLNEPIGKSVRLDIQTLTCLMMNYRRASYLARIERLETDEETLKSLE
                     RIIPNMEAYFSDYF"
     gene            33129..34964
                     /gene="lepA"
                     /locus_tag="ACG842_000860"
     CDS             33129..34964
                     /gene="lepA"
                     /locus_tag="ACG842_000860"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HFQ0247422.1"
                     /translation="MDIKKMKERQEQIRNFSIIAHIDHGKSTLADRILEMTHTVTSRE
                     MQDQLLDSMDLERERGITIKLNAVELNYTAKNGETYIFHLIDTPGHVDFTYEVSRSLA
                     ACEGAVLVVDAAQGIEAQTLANVYLALDNDLEILPVINKIDLPAADPERVRQEIEDVI
                     GIDASEAVLASAKSGIGIEDILEQIVEYVPAPSGDLDAPLKALIFDSIYDSYRGVVLN
                     VRITDGMVKPGDKIKLMSNGKTFDVTEVGVFSPKAVARDFLMVGDVGYITASIKTVQD
                     TRVGDTVTLADNPAAEALPGYRKMNPMVYCGLYPIDTSRYNDLREALEKLQLNDAALQ
                     FEPETSQALGFGFRCGFLGLLHMDVVQERLEREFNLELITTAPSVIYHVNKTDGTTAT
                     VDNPADFPEPVTIQDVEEPFVKAQIMVPNDFVGAVMELSQRKRGEFITMDYLDDYRVN
                     VVYNIPLSEIVFDFFDKLKSSTKGYASLDYEMSGYQKSKLVKMDILLNGEKVDALSFI
                     VHRDFAYERGKAIVEKLKKLIPRQQFEVPIQAAIGQKIVARSDIKALRKNVLAKCYGG
                     DVSRKRKLLEKQKEGKKRMKQIGSVEVPQEAFMAVLKMDEDEPKK"
     gene            complement(35058..36185)
                     /locus_tag="ACG842_000861"
     CDS             complement(35058..36185)
                     /locus_tag="ACG842_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFQ0247423.1"
                     /translation="MAFLFVSLNYTNGEDINDDGSRKKVEKVGGDTRPEAEAALRKVL
                     SDIDETGQYFLGTDTRVKQYLDFWMEEYVKLNLKYNTYENYRFTIKNHINGYLGKKKL
                     TDLSPALLQNFINAEFKKGYSKKTMTITHSVLKNALNMAVYPWGLIKQNPMLYVKIPK
                     YEARPTTKKDLKIISLEDFDHMLEITPEGHPFYIPLNIGFYTGMRVGEVCGLTWDDVD
                     FSNGTITVEKQMVKNDGAWVYGTPKTSSSNRTIFIGQTLLAILKKHKKQQLENRMKYG
                     KLYIDSNAVCTKEDGELVTPSVVKWNTRRISNALSLSFNFHSLRHTHATLLLENGAKM
                     KEISERLGHSRISITMDTYSHVTDKMRNETVDIMENLRKNS"
BASE COUNT        12048 a         6416 c         7738 g         9996 t
ORIGIN      
        1 ctaaaaagct ttttaaaagt gatcagtaaa aaacggaatc tgatcaaaac gacacagctg
       61 ttgaaaaaat atccaggtct gcttcacacg tctattcaat ttcctcaatt gattacgatg
      121 atccatcgtt ttatacgtat gcaacagcta ccatctaaga agctgtttct tccattgacg
      181 gacacatttc gagtagtcaa cagagaagat aaaaagaaag tgattgtgat tgactttacc
      241 agaaatcgga atgtccttca ccaagttgca atggaaaagg ccaattaagg ctttttcttt
      301 ttggattttt ttgtcataat aaatattaga tgatggagga gataatatga tgcaaacatg
      361 gttagaaagg ctaatacgat ttcagcagaa ctatcacaaa cttatgcagg gaagatatgc
      421 gaaatttgat cagttgaatc gcggtctatt gatcgctgcg cttgtcttta gtttgctaag
      481 tcgttggttg ccctatagaa tcggacaatt gttgttcatc gtctttttcg gatggatgat
      541 atttcgtttc ctatcaaaaa aaatctaccc cagactaaat gagaatcaac gttatttgaa
      601 atatcttgat agagtcaaag aaaaatatca tcagataaaa aacaaaaagc aagtcaaaat
      661 ggatcgaaaa agttatactt tttttgagtg cccgaattgt catcaaaagc agcgtgcacc
      721 taaaggaaaa ggacgaatac gtgtgacatg caaaacatgt ggtataaaat tcgaaacgaa
      781 tgtttaaggg agaaaagaat gaaattagta ttattacgtc atggcgaaag cgaagcaaat
      841 tttgaaaatt actggacagg atggctagat gtcgccttaa cagaaaaagg aaaagaacaa
      901 gcagcagttg ccggtcaaaa aatgcttgaa gccggacttc aattcgatgc cgtttatacc
      961 tctgttttga aacgtgcgat tcttaccgga caagtcgcac tagaagaaat gggtcagtta
     1021 tggcttccta taataaaaac atggcgtcta aatgaacggc attatgggga tcttgtgggt
     1081 aaaaacaaag ataagatgaa agaaatatac ggaaaagatc aagtaaaaac atggcgcaga
     1141 ggatatacgg aagttccgcc gttaaccgca gaaaatagat ttgaccgcag atatgatcac
     1201 ttggatccgc atttgatccc atacggagaa agtctggaaa tgacagtgaa tcggatcatt
     1261 cccttatggc aagatcatct agcaccaaaa ctaaaagatc ggcaagattt actagttgtg
     1321 ggtcatggta atagcatccg agcactgaca aaatatttag aagatgttcc tgaagatcaa
     1381 atggatacga tcgatatccc aaatgcacaa ccaatccagt atacattagc agatgatttg
     1441 acaattctgg acaaaaaaat actttaaaga acacctttat ctaaaagtga gagatagaaa
     1501 aaagaagcaa aggaatggaa caaaaggaac atgaaaaatt atatatggga ctttgatggt
     1561 acattgtttg atacgtatcc cgcaatggta gatggtgcat ggcaagcact aaaagatttt
     1621 ggtatcagta tggacaaaaa agaaatctac ttcaaaatga aaaagtattc tactagttat
     1681 ttgatcaatg aatcaaactt gaatgccaga gagtttaatg aattatttca ccgatatgaa
     1741 aaagaaagta cggaagtttc ccgtccattt ccagaaacga aacaagtact ggaaatgcta
     1801 aaagacaacg gcggtcgtca tttcatcctg acccaccgtt tgacagaatc aacatggggc
     1861 ctgttaaaag aacatcgatt ggcacatctg attgaggaag tagtagggat cgatcaggac
     1921 tttcctcgta aacctgatcc agcatcttta aattatttga tcgatacttt tcatttagaa
     1981 agaacagaca cgatgatgat cggcgatcgt cgtttagata tcgaagcagg gaaaaatgca
     2041 ggagtcgcta cttgtcttta cgacatcgat cattttctgg gtgagatccc ggcagattat
     2101 gtggtaggaa atctgaatga aatccttacc ttgcagagca tcattaaaaa gtagtatccc
     2161 ctttcaaagg agatatgtta taattttcat aaaggagtga taaaattata tggaaatcag
     2221 aagacaaaaa gaagttgtag aagcatttca ttatgatatg agattgccga accaagaagt
     2281 agaaacagat ttgaaagtag gcttctcgcc tttaaaagaa acgatcgaaa actatccgaa
     2341 agaacatagc atcgtaggag cacgcttaga gttccgttta gtgtttgata catatgtttt
     2401 atctggaagt gtgagtcaga tcaatcatat cctcaatcgg aaaattgaag gtcaaaatga
     2461 tgtcacacaa gaagaagtgg acgagctagt agcaccgtta ttcgatatcg tacgccgaat
     2521 gtcttatgag gtgacagaga tcgccttaga tcagcctggg gtaaaattga attttcaatc
     2581 agaaaaacaa gaatagacag taaaaacata cctgcgatag attaaagcta tcacaggtat
     2641 gtttttttat tactaattgt aagcaaaaag aatgtcattt cgataaaaat cgtctaaaat
     2701 ataaaagtca ctgaaaaaaa ttttttcagg aggatggttg aagaatcatt ctcgggaacc
     2761 tttacttcct tcgatggact tgtcattaat aatatgagga ggagaaaaat gacaacactc
     2821 atcacagttt tagtggttct agtttttgcg gcattattat atgtgttcta tcgtatgcaa
     2881 aagcgccata caaaattttc aacacgcgtc tttacagcat taggagttgg tatcgtcttt
     2941 ggcggtctgc ttcaattgat ctttggtgta gacgacaaag tcgttactca atcaatggaa
     3001 tggatcggga ttgtaggtca aggatatgta tcattcttgc aaatgctagt tatgccttta
     3061 gtgtttattt cgatcattgg tgcattcaca aaaatgaaag tttctgaaaa gctagggaaa
     3121 attagtgcga ctgtattgac ttctttactt ggaacaacag ctattgcagc attgatcgga
     3181 attggttcaa caatgctgtt cggactccaa ggagctaaat tcacacaagg agctgcagaa
     3241 acagcacgaa tctctgaact ggcaacacgt caggaaacag ttgaaaactt atcgattcca
     3301 cagcagattt tatcctttat ccctaaaaac gtttttgctg atttagcagg tagtcgtgca
     3361 acaagtacga ttggagtcgt tattttcgcg gcatttgtcg gaatcgctta tctaggcgtg
     3421 aaaagaaaag cgccaaaaga agcagcattc ttttcagatt tagtcgacag tctgtataaa
     3481 atcattatgc ggatcgtgac gcttgttttg cgtttgacac cttatggagt atttgctttg
     3541 atgacaaacg ttttggcaac aagtgatttt gcagctatcg tcaatttagg taaatttgtc
     3601 gttgcttctt acgtagcatt gcttgctgtt ttgattgtcc actcactgat tttgattggt
     3661 gcaaaagtca atccaatcaa ctatttcaaa aaagcctttc cggtattgag ctttgcgttt
     3721 acttcacgtt caagtgctgg agctttgcca ttgaatatcg agactcagac aaaagcttta
     3781 ggagtagatg atgcaacagc taatttctca gcaagttttg gtttgtctat tggacaaaac
     3841 ggctgtgctg gcgtttatcc agctatgttg gcaacgatcg tcgcaccagc agctggagtg
     3901 aatgtcttta gtttggaatt tattttgatg cttgtggcaa ttgtaacaat cagttctttt
     3961 ggtgtagctg gagtcggtgg gggcgcaaca tttgcttcac tgatcgtttt aggggcaatg
     4021 aacttgccag tagcaatcgt tgggttagtt atctctgttg aaccattaat cgatatggca
     4081 cgtacagcag taaacgtcag cggtagcatg gtagctggta tcgtgacaag tgcacgcatc
     4141 aaagatttag atcgcgatgt catcaatgat gattctaaag tgatcgatgt gaatgcatag
     4201 tcaaaaagag gttgtgatag aagtggtcag ctttaagaaa taagaagaag tttcctaaaa
     4261 atagtttctc aaatttttgt gaaatttcgg cttatttccg agaagctact tctgaaacac
     4321 cgtgaataag aggttgtgaa aaagctgccc tgcatcaagt aataagaaca gccaaacaaa
     4381 aatagttcct catattttac gtttggttga gcttattacc gcagatgcaa gcttttgaac
     4441 accgtttaag aaagaggtcg tgagcctgcc gtttcactct gagtcacaaa gagcaatttc
     4501 aaaaaatagc ttctcatgtt tgttgaatat tgtgtcttgt gaccgaggag ttgtcaggag
     4561 aacaccgttt accagaggtt gtgacttgtt tttagtcacg acctcttttt ttatttattc
     4621 catataaata cacattattt attccgaaaa gtgaaagcgc aaataacaaa aacaaccaac
     4681 ttatttatta ttaaagaaaa gcgtatttaa gtaggacgat tctgttgaga gctttcgtag
     4741 attttcccat tttcctatgg taaactgaca aagttgagaa tgttagaaca gatcgtttag
     4801 gaggaaacag atgtttacga atgaaataaa aatcatgctt tatgtggacg atgtagaaaa
     4861 aaatgcaacg ttttggagag cagttggctt tgcggaaaaa gatcgtcaag aaatggatgg
     4921 gacgcttata attgaagtag gagttgagga cagtcaaacc tcatttctgt tatatgatcg
     4981 agcatttgtt gagtcccatt cacctggcac agctactagt gccccatctt tgatgtttta
     5041 cagtaaagat atctttggac tatataaaaa aatgcagacg caatcagtca cattaggtga
     5101 tatggtacag cttggagaag aatatgtctt taactttgct gatcccgatg gcaactactt
     5161 tgcggttaca ggtaaataat agaaataaag actttgtttt tctgtgcgaa acaggaaagc
     5221 gaagtctttt taattcaaat aataatatta tgtaaacaaa aaactttcca tgcgtaatta
     5281 tacacaagca aataaatcgt caaatgcgaa cgccttcttt atactgttta ggaacgaaaa
     5341 gaaggaagga agaaatacat ggattatcaa gtactgcttt attacaatta tacaaaaatc
     5401 gaagaaccag aggaatttgc caaaaatcat ttagcctttt gcaaatcttt ggatcttaaa
     5461 ggtcgtattc tagtagctga agaaggaata aatggtactg tgtctggaac cgttgaaaac
     5521 acgactgcct atatgaaagc aatgaaagct gatcctcgat ttgctgatac gatgttcaag
     5581 atcgatccaa gcgaaggaca tgcctttaag aagatgtttg taagataccg tccagagctt
     5641 gtaagtttaa agctagaaga ggacattgat ccaaaccaag tgacaggtgc ctatctttca
     5701 ccgcaagaat ttaaagaagc tattttagat gaacagacag tggtgattga tgcacggaat
     5761 gattatgaat atgacttggg acattttcga ggggctgtac gcccagatat acgcagtttt
     5821 cgagagcttc cagactggat ccgcgagcac aaagaagaat ttatggaaaa acgggtagtg
     5881 acctactgta ctggtggcat tcgttgcgag aagttttctg gttggcttgt ccgtgaagga
     5941 ttcaaagatg tcggacagct gcatggcggt attgctactt atggtaagga tcctgaagtc
     6001 caaggagaat tatgggacgg caagatgtat gtgtttgatg aacggatcgc tgtcgatatc
     6061 aaccaagtag ataaacaaat cgtcggtcgc gactggtttg acggaacacc atgtgaacga
     6121 tatatcaatt gtagcaatcc tgagtgcaat cgtcagattc ttgcttccat tgaaaatgaa
     6181 gaaaaacatt taggagcatg tagcatagag tgtgcgaaac atccgcgcaa tcgctatgtt
     6241 caagaccatg gaatctctcc agaagaagca gaactgcggt ttacagcttt aattaaatag
     6301 aaaacaatag ttttgattgt cgaccatttc caatatgcgc tatactagcg ttgaggatgg
     6361 tgatccacat gaaatcattg atttatgcta ttggggatgt ccatggcaaa catgagcttt
     6421 ttgaaaaact tattcactat tatgatccaa agatccatca gcttgtttta ttaggggatc
     6481 tcaatgatcg aggtcctcgt acaaaagaat gttttttatt aggaaagcaa ttagtcgaag
     6541 aaacaggagc catttacctc cggggaaacc atgaagaata ttttttgcaa tttctgcaat
     6601 caccggagga ctggtatgcg ccttatatga gaaatggtgg aaaagaaacg tttgaaagcc
     6661 tgattcaccc aggggcatcg gaggaatact ccccaactga aatctcgatg atgatccaat
     6721 cgagatattc agaactgatt aaatttttag tccagcgccc cttgtattat gaatgggaaa
     6781 attatctttt tgtccatgcc ggtgtggatt tgacaaagaa agactggcgt aagacaagtc
     6841 taagagactt tatttggata agggaaccat ttcatgaagg gaaaaacaat acaggtaaga
     6901 caattgtatt tggacatacg attacaccaa tgctccatgg agatatgcag acaacagacc
     6961 tctggattca ggatcataaa atcggaatgg acggtggggc tgtattcgga gggtcattgc
     7021 atggggttgt ttttgacaag gaaggtattc ttcaagatat tgaatatcag aacaacgaag
     7081 gaccttggca tggaatctga ctaatggaaa gaatgaagga gctgtgactt tgttgcggct
     7141 ctttttattt ttatagagaa tattgtttct ttcagtagtt taacccagga taaaaagtat
     7201 gatagaataa agaagcaatt tttagcagaa aagaggcatt ttaatgacgg aaaatttgaa
     7261 ccagacgatc attcagcttg tacaaaaaga actttcagac tatcgtccta agcagttgac
     7321 tactgtactt aatttgttaa atgaaggaaa cactgttcct tttatcgcgc gttaccgaaa
     7381 agaaatgact ggaagtcttg atgaagtcca gattcgtgaa atcgaagagc ggtatgccta
     7441 tcttgaaaac ttagaaaaac gtaaaactga agtgattcgg ctaatcgatg aacaagggaa
     7501 actgactccc gagttagaag cagaaatcac ccaagccgtg aaaatgcaac aagtggaaga
     7561 tttgtatcgt ccatacaaac aaaaacgacg gacaaaagca acgattgcta aagaaaaagg
     7621 acttgaacca ctcgcgaagt ggctgatgca gcttacagat ggagaggtgc aatctgaagc
     7681 agaaaaatat attgacaaag aaaaagaagt ctcttctgcc gaagaagcgt tgcacggtgc
     7741 tcatgagatt attgctgaac aagtcagcga taatgcaaaa tttcggactt ggattcggtc
     7801 ttatacgtat aacaaaggta tgtatgtcag caatgtcaag gatgagcaag cagatgaaaa
     7861 aggcgtatat gaaatgtatt atgactttgc agaacccgtc cataaaatgg tttctcatcg
     7921 aatattagct actaatcgtg gagaaaaaga agatattctg aaagtattct ttcaagtaga
     7981 tgaagcagca attttagctt acttggatcg ccaaatagtc aaaaatcctg cctctccttc
     8041 ttcttcattc gtcagagaag cctatcagga tagttataaa cgctttattc agccagcaat
     8101 cgagcgagaa ctgcgcaatg aattgactga aaaggcagat gagcaagcaa tcgctatttt
     8161 cggcgaaaac ctccgtaatc ttttgttgca accgccacta aaaggtaaag ttgtcctggg
     8221 atttgatccg gcttatcgta ctggatgtaa actagcagtc gtagatgcga cagggaaagt
     8281 tttagcaatc gaagttattt atcctcataa acctgcagcg caagcaaaaa gagaagctgc
     8341 tggaccagct tttatccaat taattaataa atatcaagta gatatggtag ctatcggaaa
     8401 tggtacagcc agtcgggaat ctgaactttt tgtggctgag cagttgaaat ccgccgacca
     8461 taaaacctat tacgctatcg tcaacgaagc aggcgcttct gtttactccg ctagcgaaat
     8521 tgcacgaaaa gaatttccgc atctacaagt tgaagaaaga agtgctgttt ctattgcccg
     8581 tcgcctgcaa gatcctttag ctgaattagt caaaatcgat cctaaagctg tcggagtagg
     8641 ccagtaccaa catgacgttt ctcaaaaacg tttggctgaa caattggatt ttgttgtgga
     8701 aacagcggtt aaccaagtag gtgtggatgt gaatacagca agcccgcagc tcttgcagca
     8761 tatatcagga ttgaataaaa caactgccca aaacattgtc atttatcgtg aagaaaacgg
     8821 agaattcacc gcacgaactc aattgaaaaa agtgccacgc ttaggaccaa aagcatatga
     8881 acaagcaatc ggattcttgc gagtccctgg tgggaaaaac attttggaca acacaggtat
     8941 ccatcccgaa agttattcta ttgcaaaaga aatattaact tcagtccaac tttcagaaaa
     9001 agagcttggt acagaagaag cagtagaaaa attgacacga ttatccgtag agaaactggc
     9061 tgaaagccta tcagtcggag aagaaacgtt agccgatatt ttagctgggc tgacacaacc
     9121 tggacgagat atgcgtgatg agatgccagc gcccttatta cgtacagatg tattgagtat
     9181 ggaagacttg aaaccaggga tggaattgac tggaacggtc cgtaatgtca ttgacttcgg
     9241 cgcatttgtt gatatcggtg tgaagcaaga cggacttgtc catatatcga aactcagcaa
     9301 aaaattcgtc aagcatccta cagatgtcgt gtcagtagga gatatcgtaa cagtttggat
     9361 agaacaagtc gatacgaaaa aaggcagaat cagtttgacg atgctttccc cttatgaaga
     9421 gtaacaatga ggagtaacaa aaattgacac aagaagaact gcagcagtta gtagaagaga
     9481 tatcattaaa aagcttccag cttcctttca agcaccaagc aatgttcaat ccacgcttac
     9541 gtacaactgg cgggcgctat catttaaacg atcatcattt agattttaat gcgaagctat
     9601 ttgaagagtc agacgaagag acgattgcag gaatcatcaa acatgaactt tgccattatc
     9661 atcttcatct agctggaaga gggtatcagc ataaagatat ggatttcaag tatcttttga
     9721 aaaaaacagg agggttgcgc tatgcaccag ccttgacgaa gaaacagata aagatggagt
     9781 attatcgatg ccaagattgt tctgccacga tctgccgcaa gagaagaatc gatgtacgca
     9841 aatatcgttg tggaagatgt catggagaaa tcaaatggat tgagacaaaa agagaagact
     9901 ttaatgaagc acaaaaaatc agatgaaagg atagaaggcc tttggaaaaa agtgagcaga
     9961 ggaatctaaa agactggaat cagctattaa attttttagc cgcacctgaa gagggaaatt
    10021 ttgaggaact gacggcacct acacttattt tagctggaaa ctgtcttcct atcttagctg
    10081 ataaagcagc acaaatgtat ttaaatggcc aagtagatca attgtttttg gtcggagggg
    10141 tggggcatgc gactagaatc ctttatgaga attttgaaaa gcaaggattt cattttgaag
    10201 atggcatgag tgaaagtgaa atctgtcgtc agtatctaaa agaagtctat gatttgcccg
    10261 ataaggcttt tctgatagaa tcgaagtcta caaattctgg agagaacgcc actttttcac
    10321 tggagattct tcattcctta ggagctgtac cagaaaaaat cttattgatg aatgatccga
    10381 cactgcaacg caggactcga gcaacttttg agaaagtatg gcaaaatgaa caaactattt
    10441 tcgcgaatgc tgtgccattt gtgccggaaa tccttcactt ttctgaagag atcatcttta
    10501 cagcaaaaga actgaatcat caatggccta aggaatattt ccatgcgcta gtattaggag
    10561 aaatggaacg tctccatgac gatgagaacg gctatggtcc gaaaggaaaa gattttattc
    10621 ctcacattga tatatcagag gaagtttggt cttcttacac cagaattaaa gagtcaataa
    10681 aaacggattt ttctcgtact tgaccatttt agcaagcgaa tcctcctttc caaataaatt
    10741 tggttgaaat tttcaatcgt ttataagata atgaggagcg tgttgaatgt aatagctaaa
    10801 agaatggacg ctagataaga gaagaggatt caaatggaaa atcaaaataa attgtatcat
    10861 tttgttggta tcaaaggttc aggaatgagc tccttggctc ttgttttaca tgaacaaggt
    10921 ctaaatgtcc aaggatcaga cattgaaaaa tatttcttta cacaaagaga tttagaaaaa
    10981 gcaaatatca cgattctgcc atttaacgca gataatgtaa aaccagggat gacgatcatt
    11041 gcaggaaatg catttcctga ttcacatgaa gaaatccagc gagcaaaaga attaggtttg
    11101 gaagtgattc gctatcacga ctttattggt cattttatcc aaaattatac gagtatcgct
    11161 gtaacaggct ctcacggaaa aacaagtacg actggactgc tctctcatgt attgagtggt
    11221 gtccgtccaa caagttatct aattggagat ggaacaggac atggtgatcc gcaagcggaa
    11281 ttcttttcat ttgaggcctg tgaatatcgc cgtcatttcc ttgcctattc accagactat
    11341 gcaatcatga ccaatatcga ttttgatcat ccggattatt atacaagtat cgacgatgta
    11401 tttacagctt tccaaacaat ggctggacaa gtgaaaaaag ctatttttgc gtatggtgac
    11461 gatgcttatt tacgcaaatt aaaagcaaat gtgccgattt attattatgg tgtcacagaa
    11521 aacgatgata tccaagcacg aaacatcgaa cgcacaactt ctggttctgc ttttgatgtc
    11581 tatcatgggg atgaatttgt tgggcacttc actgtaccag cattcggtaa acacaatatc
    11641 ttaaatgctt taggtgttat tgcagtagct tatttcgaaa aactggatct gaaagaagtt
    11701 gcagaagaaa tgctgacttt cccaggagta aaacgtcgat tcagtgaaaa gatcgtagca
    11761 gacatgaccg tagtcgatga ttatgcgcat caccctgcag agatcaaagc aacgattgat
    11821 ggcgcacgcc aaaaatatcc agataaagaa ataattgcag ttttccaacc ccatacgttt
    11881 acgcgtacga ttgcattgat ggatgaattt gcagaagcac ttgatttagc tgataaagtt
    11941 tacttatgcg atatttttgg ttcggctcgg gaagaacaag gaaacgttaa gattgaagac
    12001 cttggagcga aaatcaaaaa aggtggagaa gtcatcaaag aaaataatgt atctccattg
    12061 ttagattatc atgatgcagt agtaatcttc atgggtgctg gagatgttca aaaatttgaa
    12121 caagcgtatg aaaaactttt aagcagcacg acgaaaaatg tactgtaata caattactgc
    12181 agtgtcctta taaaaataga aagaggggga gtggcaaaaa tcttgccaag cccccttttt
    12241 tgcaacttat gataaacggt gttcaaaagc ttgcatctgc ggtaataagc ccaatcaaat
    12301 caaaaacatg agaagctgtt ttttcttggc tgttcttatt atttgatgca ggacagcttt
    12361 ttcacgacct ctgtaaacta taggaatctg ttaaatcaag ggagcagaac ttgcctttta
    12421 cgctactttt ggtacaataa tgtatgatta aaaataaact ggagggtttc tatcttgaac
    12481 ataggtaagc cgagaaaact aggaaaaaca ataaatgaaa tcgaggaagg cgactcgtta
    12541 tccttaacag aatctattga agataaccag cttttgctgt atttgggatt gacgaatgat
    12601 gcaaatcctt tgtatatcca acatgacttt gcacaaaaaa cagaatatca acagccaatc
    12661 gttccatcag tgatgctgat ggggatcatc acaagtgcaa tttcaaagca tcttcctgga
    12721 ccaggatcac atgttgtcaa tttttctgtc aattttattg aaccggtttt ccattatgaa
    12781 acactgactt ttcagtttga ggtcataaaa gtagataaga tgaaggatgt ggtgacgatt
    12841 tctgtagaag cggagaatac gaacgaagat cgtgtgttgg atgctgtcgt tatggtccaa
    12901 ccgccacaag ttcctgaatt ggaaatagca atcgaaagtg aaggtgaatt gaatgaataa
    12961 tccaagtaca tcaatgtcag gtatcaatcg tttttatgca aaagtttatg gatttttagg
    13021 tatgggactt gcccttagtg cagtcacttc ttacttggct ctttgggtat ttcctttgca
    13081 attagcaact tttatcaata attttccact aggctttgtg ggtctgtgga tcgttgaact
    13141 gattttagta atggttctag gcgtaaaagc acaaaagaat ccgagcttag caatcggtgg
    13201 atttattgcc tactctcttt tgaatggcat aacattagcg gttactcttg catactacga
    13261 tattggaaca gtaacgcaag ctttcttgac agctactttg atgttcatgg ggatgtctgt
    13321 tgtcggagta acaacaaaaa gagatcttag cggggttgga agagcaggac aggcagcttt
    13381 aatcggagtg atcattgcac tagtcttgaa catcttcatt ttgagaagta gcgtagttga
    13441 gctgttcatc tccctactgt tagtcgtcat ctttgcaggg attacagctt atgataacca
    13501 aagcatccgt aatatctatg tccagacaaa cggaacagca catagtggaa ttgctgtatt
    13561 catggcattg aagctgtatt tggactttat caacttgttc ttggcgtttc tgagaatctt
    13621 tggacggaac aactaaaaaa gaaaaaaatt catcagttgg ggctataccg aattatttgg
    13681 tatggtcccg ctttttcttt tcgcattccg caggattctt tgttagaatg aataagaact
    13741 ttacagtaag gagttaagaa gatgacaaaa aataaattat tactcgttga tgggaacagt
    13801 gtggcttttc gcgcattctt tgcgcttcac aattctcttg aacgtttcaa aaatagaaac
    13861 ggtcttcata caaatgcaat ctatgcattc aatacgatgt ttgaaaatgt tatgcaaaaa
    13921 gagcaaccga ctcatgttct ggttgcgttc gatgcaggaa aaacgacttt tcggacagaa
    13981 atgtatgcgg agtacaaagg tggaagatcc aaaacacctg gcgaattcaa agaacaaatg
    14041 ccatatatac gtgatttgtt gacaggacta ggggtacagt attacgaatt gcccaattat
    14101 gaagcagatg atattatcgg tactttggca gaaaaagtag acaaagatca atttgatgtc
    14161 gtcgtgcttt caggtgatcg ggatttgact caattagcta gtaaagaagt aaaagtggat
    14221 atcactgtaa aaggtgtcag cgatatcgaa agctatacac cagaacatgt agcggaaaaa
    14281 tatgatgggt tgacgccaaa acagattatt gacatgaagg gactagccgg agatgcctct
    14341 gacaatatcc ccggtgtgac taaaatcggt gaaaaaacag ctatcaagct attgaaacaa
    14401 tatggctcag tcgaaggaat ttatgaacat atcgatgaaa tgaagcaaag caaaatgaaa
    14461 gaaaatctga taaatgacaa agagcaggct ttcttatcga aaaaattggc gaccatcaat
    14521 acttcagcgc ctgtggatgt ctctatcgac tcattgaagt atgaagggaa aaatctcgat
    14581 aaactcgttc cgttttataa agaaatggat ttcaatcagt tcctttctaa attgaatatc
    14641 gtagaagaag aagtaaaaat ggatgatatt ttgtttgaag tcgttcatga attcaaagag
    14701 gaaatgttta ctacggatat ggcgttgtac gtggaaatga tgggcgacaa ttatcataca
    14761 gaggaaatcg tcggagtagc gtgggggaca gaaaagaaaa tttatgtcac gaacgatcta
    14821 tctgtattcg aaaaccgagc gttccattct tggatcactg atccgactcg gctgaaaaaa
    14881 gtatatgatg cgaaacgaac ttatgttgct ttaaaccgct atactggaaa aacgaaagga
    14941 atcgattttg atgtcttgct aggtgcatac cttctcgata cgaatgaaaa aagcacagat
    15001 atcgaaggaa tagctgctca ttacggatac aacgatatcc aatcagatga agctgtatac
    15061 ggcaaagggg cgaaaaaagg tttaccagaa gaagaggaac tcttttttgc tcatttagcg
    15121 cgaaaagtag ctgcaatcaa tgcgttgaca ccaaaattga gccaagaact ggcagaaaaa
    15181 aaccaagaag atcttttccg gaaaatggaa ctgccattgt ctatcatttt ggcagagatg
    15241 gaaataagcg ggatcaaagt agatgcgact cgcttacaag aaatgaaggg cgagttctct
    15301 gctcgcttaa gagagatcga acaaaaaatc tacgaagaag ccggcgaaga attcaatttg
    15361 aattcaccaa agcaattagg agtgatctta tttgaaaaaa tgggattacc cgttatcaaa
    15421 aaaacaaaaa ctggatattc cacagccgta gatgtactgg aacagcttcg tgaacaagca
    15481 ccaatcgtag aggatatcct cacctatcgt caaattgcaa aaattcaatc tacctatgtc
    15541 gagggactgc tgaaagtgat ccaatcagat ggaaaagtgc atacccgtta tgtccagaca
    15601 ttgacccaaa caggacgatt aagctctgtt gatcctaatt tgcagaacat cccgattcga
    15661 ttagatgaag ggagaaagat acgtcaggca tttgttccta gagaaaaaga ctggctgatt
    15721 tattcttctg actattcaca aatcgagtta cgcgtacttg cgcatatctc aaatgatgaa
    15781 catttgaaag ctgccttcct agaaggtcaa gatatccatg ccagcacagc gatgcgtgta
    15841 tttggaatcg aaaaggcaga ggatgtcaca ccaaatatgc gtaggcaggc aaaagcagtg
    15901 aattttggta tcgtgtatgg catctctgat tacggacttt cacaaaatct aggaatcagc
    15961 cgaaaagcag cacagcaata catcgatacg tactttgaaa aatacccagg tgtcaaagaa
    16021 tatatggaaa gaatcgtacg cgaagcaaaa gaccaaggct acgtcgaaac gctgtatcac
    16081 cgccgtcgat atctgccaga catcaattca agaaattaca atatccgttc ttttgcggaa
    16141 cgaacagcta tcaatacacc aatccaagga agtgctgctg atattttgaa aatcgcgatg
    16201 atcgaattgg ataaacgatt aaaagaaaca ggtcttcaag ctacgatgct gttacaagtt
    16261 catgatgaac ttgtcttcga ggtaccggaa aaagaacttg aatcattaga taaattggtg
    16321 aaggaagtta tggaacaagc tgtttctctt catgtaccac tgatcactga cagcagttgg
    16381 ggaaaaacgt ggtacgaagc aaaataaaca ttgtttaaga cagagcaata taatgaaggt
    16441 gaaaagaaaa aatgccagaa ttaccagaag tagaaaccgt tcgtaaagga ttggaacggt
    16501 tagtagtagg aaaaacgatt caaaaagttc aggtcttgtg gcccaagatc atcgagcaac
    16561 cagagacacc catctttgag gcttctcttg ttggcgaaac catccaatcc attggacgta
    16621 gagggaagtt tttaattttt catctcgatc attgtgaact gatctcacac ttgcgaatgg
    16681 aaggaaaata tcaatttacg aaagaaaata caccaatcga taaacatacg catgtacttt
    16741 tcttttttga ggatggaagc caacttcgct acaacgatgt acgaaaattt ggtcgtatga
    16801 caatcgtcga aaaaggagca agtgcaactt accgaggaat catgaaattg ggacctgagc
    16861 ctttgccaga tagcttctta ttagcagatt tcgcaaatgg actgaaaaag tcccacaaag
    16921 caatcaagcc attgctgcta gaccaacgtc tagtgactgg tctaggtaat atttatgtag
    16981 atgaagcatt atgggaagcg aagatccatc cagaacaacc ggcgaacaca ctgcgcccca
    17041 aagaggtaga acaattacgt ctatcgatca ttgatgtttt ggatcgtgca atagaagccg
    17101 gaggtaccac gattcgcagt tatttgaatg cattaggaga atcaggcgga tttcaagtag
    17161 cactgcatgt ctatcagcaa actggcaaac catgtatacg ttgtggaacg ccgatcgtga
    17221 aaacaaaagt ggcacaaagg gggacgcact attgtccgaa ttgtcaaagg ctaaaaatcg
    17281 caaagtagga tttgttttag gtttgacggg tggaatagct accggtaaaa gtacggcagc
    17341 caaagtcttt cagtctcatg gatttccttt gatcgatggc gatgtgattg ccagagaaac
    17401 tgttgagcct ggaacgcttg ctctgaggaa aattgtatca gtgtttggcc aagaggtatt
    17461 gcagcctgat gggcaactag atcgagggaa acttgggatg attgtttttc catcaaagga
    17521 aaaacggcaa aaacttgatc agctattaga tccgtttatc cgaaaagcga taaaagatca
    17581 aatcgcctct ctaagttcca atcatccttt agtaattgta gatatcccat tgctctacga
    17641 aggtcattat gaccactata tggatgcagt agctgtcgtt tatactacgc cagaaactca
    17701 gcttcagcgt ttgatgaaac gtaatcaact gacgctagaa caagcgcagc agagaattag
    17761 tagtcaactt ccaatcgaag aaaaaaagca gcgtgcggat attttgttcg acaacaatgg
    17821 aacaaaagaa aaacttgtgg gacaaatcga aaattggctt tttgctaatc gctttctttg
    17881 agtatctgtg tcaattttgc tatcctgagt atatagaaag tgagggatag cgatgggatc
    17941 attaaaaaat caaaaggata aagtcgtagg taacgcaaaa gaaaaaatcg gcgagatgct
    18001 caataaagaa aatctaatcg atgaaggtcg tacgcaatca gaaacagcag aagaaaaaga
    18061 aaagcagtac caagcaagaa tcgatgaaat agaagaccag caggcagaag aagaagcgaa
    18121 gcggcaagat ttgactcaag aacgaaaaaa tccgacacct gatcgtagta aaacaccgct
    18181 cccagaaagt gaccacattc ccgatgagca aaaaatcgag gaacagcgaa tgaaacacta
    18241 tacaggaatc gaagagaagt tataaaatag taaaaaatcg agatgtgact tgaagcagat
    18301 gagaaggtgt tcatctgctt caagtttttt gtgctagatg actaaatcag cttttgtgta
    18361 agttatttct cagtaatcaa ggtgatcagt attggaaaat tgaaatttag taaggacagg
    18421 ggacacaatt acgcatgaaa gtagaaaaaa ttgttcttat atccgtgttt tttgggtagg
    18481 acagtcgcgt ttgtgatttg tttcgtgtta taatacaact agttgtaaag aaagacacat
    18541 cctttatata aaatgcgatg caagaggtga gacaaaatga gatgtcccag atgtcaacat
    18601 aataattcac gagtaatcga cagccgtcaa gcagatgatg gacgtgcgat ccgtcgccgt
    18661 cgcgaatgtg agaattgttc gttccgattc actacatttg aacgaattga agctgctccg
    18721 ttattagtaa tcaagaaaaa cggggaacgc gaagagttca atcgcgataa aattttaaga
    18781 ggactcatcc gttcagcaga aaaaagacct gtagctatgg aacaaatgga acaaatcgtc
    18841 aaccatgtcg aaaaccgcgt acgcagtctc ggagaaaacg aagtatcgac gaatctaatt
    18901 ggtgaatatg tgatggaaga tttagttgat ttagacgaaa tcgcttatat tcgttttgca
    18961 agtgtttatc gccagttcaa agatatgtct gttttcttaa aagagttgca agatatcgtg
    19021 gataaagcaa aagaaaataa taaagagaat aataaagaaa acaattaaga aacaaaaagg
    19081 aggcatttcc ttggaaaatg cttggaaaga gcttcaacca aaaaatattt accaaggaag
    19141 aaaagcggct ccgctgagag aagacagcaa gaacgccttg ctgtatcttt atcagccact
    19201 aatcggcgga gaagccttga gtatctattt caccctgtta tcagaaatct ctcaagaatc
    19261 tggcaaaggt cctgaaggga tgcatgcaga tctgcttagt agtctaggct gcggtatccc
    19321 gcaattttat gatgcaagaa aaaagctaga aggaatcggc ttactggatg tttattttaa
    19381 agaagatagt gatctgggac ctcgttttat ttatgagctg ttagaaccac tcattcctaa
    19441 agcatttttc aaagactctg ttctggcttt tcttcttctg gaaaaagttg gtgaacgacg
    19501 attccagaag cttacccaat tctttgaacc taaaaggcat acagcagagg gatatcaaaa
    19561 aatcacaaaa aaatttttag atgtttatcg cttcaatgaa gcagtgtacg ctgctaatca
    19621 agaaaacatc gaatcgctga ataaatcttt tgaagaatct gaatcagtag cattacttga
    19681 agcagattcg acattcgatt ggcagttttt gaatgattgg ctggcaaaaa aacatattgc
    19741 aagtcttgat gatccaatca tcaaacaagt cgacatgtat catcagcttt acggtttcga
    19801 tgaattgaca cttggcgaac tgatcagtca atcttatgac tttagtgaac aaaaagtatc
    19861 tgaaaaagaa ctgcaaaaag ccattttggc aatgcagaga cgtccggtga agcaaacaaa
    19921 agaaacagtt gcggtttctg aaaacagtaa tgaaccctcg tcaaacgaac atttgacaaa
    19981 cgcagcgctt cagctgataa aagaagcaca gagcattcca ccgatgaaat atctggaagc
    20041 aatcaagtca gaaaaaggcg gatatgtttc taaacaagaa aactggttac tgcaagatct
    20101 tgtttctcaa tcaggtcttt ccagtagcgt gatcaatgtt ttgctgaatt atgtactagt
    20161 gatcaagaat aatgctagct taaatgccag ttatgtaaat acagtggcaa atgaatgggc
    20221 acaaaagaaa atcgccacag cagaagaagc gatccagcac attcggcaaa taagtaatca
    20281 agcaaaacaa tcaaaacaaa agaagcaaac aaattatcaa actggcaagc gaaatgttcg
    20341 acgggaaaag cttcctgatt gggtcaacca gccaaaagat gaaaaacaga tcagcagcga
    20401 gaaaaaagca gagattgacc gccgttttaa agaatacctg gcaaaaaagg agggtgatat
    20461 ctagtggaag acgtaggcaa agaattgaag aggatcatca gcaaacgcaa ttatcaagaa
    20521 aagtatgctg aaatgatcgc tgaagtctta aatgatgcgg atgttcaagc ttttctaagc
    20581 gcacaccaag aagaattgac acaagcggat attgaaaaaa gttacgctaa attgtatgaa
    20641 ttcgtgcaag aaaagcgtaa atatcaaaaa aacgatccaa cgatgatcgc tcccggctat
    20701 gagccgaagc tgacgttgaa ttttcattat gtggatgtca cttatgtacc aacggaagca
    20761 ttgttagcac ggcaaagaca agaagaaatc agaagccgag taaaagcaat ggatatgcca
    20821 aaagatattc aatcggcttc ttttgaaaac tttgaacgta cagatggacg aggatatgcg
    20881 agcttagaag ccctagattt tgtagagaaa tatactgctg atccaaaagg gtatcataaa
    20941 ggactgtatc tagtaggaag ttttggtatt ggaaaaacat atttattagg agcaacagcg
    21001 agagaattag cgattgctgg attcaccacg acgctagtcc attttccgac atttgccgtt
    21061 gagatgaaac aggcaatcgg aaaagatcaa gtggcagaaa agttagatgc agtgaaacgt
    21121 tcacctattt tgatgatcga tgacattggt gcagatgcga tgagtagttg gatacgagac
    21181 gaagtattcg gtgttatcct tcaatatcgg atgcaggaac agttaccaac cttcttctca
    21241 tcgaatttta caatggatga attggaacag catttgtccg tcactcaacg tggagatgaa
    21301 gagccgctaa aagcgaaacg gataatggaa cgcatacgtt atttgacaaa agaaatcgaa
    21361 atgactggtc gcaaccgacg caatggttga acagttgaca agtccaactg acatttgacc
    21421 tagtagtaaa agcaggtcag cgaatcatta tatggacaga aatgaccgga gacaatcacc
    21481 gcaacaagta atgcagaaac ttaaacggag gattttttat gagggctatt ttttctagta
    21541 tatctgtagt tggtctattt tttcttttag caggctgcgg tagcgcagat agcaacacag
    21601 gttctgttga ttcgattgat gcgggtcaaa cagtaaaaac aagcagtacg aaaacggaga
    21661 gacaaagcat atttgtcgga acgattgaaa gtatcactca agtagaagca gaagaagaaa
    21721 cgcttcagtt gaaactcgtc gatgtcatgg ccaaagaaga tccagcgaat atgggaacgt
    21781 cattcagcaa tgacggagtg atcttaaatg ctactgctga aaaaataagc gtggacataa
    21841 atgaattgaa aatcggtgac aaagtccaat ttacattagt tgagatgccg atcatgacga
    21901 tgtctatccc accgcaagtt cctggagatt cgattattga tgtttcattg aacaaataac
    21961 caataacatt ttttcaaaaa taagaaaatg ttgttttata gaacgaaaag gtatattttt
    22021 taaggagata tgggaatttt tttattccta gtctccttat tttatttgga aaatgctata
    22081 attatctaga aggagtgaag ctatgaacca tttagattta tctcagcgct tagccgcagt
    22141 gggtgacttt gtccctgaat ccgctcgttt agcagacatt ggatcagatc acgcttactt
    22201 acccgttgca ttgatattaa gaggaaaaat cgaatatgca gttgctggtg aagtcgttca
    22261 aggtccttat gaatcagcac gtaagcaagt caaaaaagat ggattagaag accgtatcac
    22321 cgtacgcctt gcaaatggtc tagatgctat ccagccacaa gatggtatta cagctgtcac
    22381 gattgcgggt atgggaggtt cactgatccg agatatccta gaagctggca agttgaatca
    22441 acgcttaagc ggacaagaac gtttgatcct tcagccaaac atcggagagc gtacattacg
    22501 atcttggctg caagaaaatc agtataaaat ccaatcagaa aaaatacttg aagaaaataa
    22561 aaaaatatat gaaatcattg tagccgaaaa agaacaaaaa ccagttactt acaatgaaat
    22621 ggagctgatg ttcgggccat ttctgcttga agagaaaagt gagatcttca aatcaaaatg
    22681 gacaagagag cttcaacaaa gaaaagctat ccgtgaacag ctcaaacaag cagctaatca
    22741 agaggcacgt caaaaacaaa tcgagcagga aatccaaatg attgaggagg ttttaaagcc
    22801 atgacaatca gcggacgcac tttcatccaa aagtttgaaa gctattgtcc tcagtggcta
    22861 gctgaagaag gagacccagt cggactgcat atcggaacat tggataagcc aatccaacga
    22921 gtaatgatga cattagacgt tcgtccagaa gtggtcgaag aagcaatcaa gaaaaaaatc
    22981 gatttgctga ttgctaaaca tcctccaatc tttcgtcctg taaaacggct ggttactgat
    23041 cagccacaag aaaaaatgta tgcagatttg ttgaaacatg atattgccgt ctatgcagca
    23101 cacacgaaca tggatattat ttgggatgga ctgaatgact ggttttgtga acttctcggt
    23161 attgaagtgg agagttattt agtaaagact catgagattc attataaaaa actggccgtt
    23221 tatgtacctg tggatcatgc acagaaaatg cgtgaagtat tagctgcagc tggtgctggt
    23281 acacaaggtg attataccgg tacaagtttt acctcgattg gacacggtcg atttactccg
    23341 gaagctggtg cacaaccagc gattgggaaa gtagggaaga cagaacaagt tcaagaggca
    23401 aaagttgagg ttattttgcc ggaaactatc gaaaaacaag tgattcaagc catgcggtca
    23461 gctcatcctt atgaggaacc tgcttatgat cttttcgcta tagatgaacc agtcgaaatg
    23521 tttgggttag gacgagtagg tgaattgccg caagaaattt ctatagaggc atttgtagaa
    23581 caagtaaaag aagcgttcca actagatgga ctgagaatag tccagccaaa aaatgcaaaa
    23641 tcctctgtga agcgcattgc gatttgcggt ggaagcggtg aaaaatttta tccgcaagca
    23701 atcgcccaaa gagcagatgt ctatatcacg ggagatatct attatcatac ggctcatgat
    23761 atgcaaagcg ctgggttgat tgcaattgat cccggtcatt atattgaatc gttatgcaag
    23821 caacgattta tcgagaaatt tgaatcttgg aaacaagaag aaaattggga tattaacttt
    23881 ttcgtttctg aaacaaatac aaacccattt caatttaatt aaaaggagag aatactatgt
    23941 acgaaaatct tttgccacgt tttttacgat atgtcaaaac agaaacacgc tccgatgcaa
    24001 caagcaaaac gactccatcg actcagacac aagttgcttt tgcgcaagaa ttgaaaaaag
    24061 aattagaaga gttaggctta gctgatgttt tctataatga agataacgga ttcgttatgg
    24121 gaacattacc aagcaatgtt gatcatgaag tgcgttccat tggatttatt tcccacatgg
    24181 atacagcaga ttttaatgca gtaggagtca atccacaaat cattgaaaac tatgatggcg
    24241 agtctacgat cgtactagat gcggaagaac gctatacatt gaatacaaaa gattttccaa
    24301 atttgaaaaa ttatgaaggc cacacattga tcacaacaga tggcagtaca ttactgggag
    24361 cagatgacaa atcagggatt gcggaaatca tgactgcaat ggagatcttg atcaataatc
    24421 cttctatccc tcacggtacc atcaaagtgg cttttggtcc tgatgaagaa atcggtgtcg
    24481 gagcggataa attcgatgtt gagcaattcg atgtagattt tgcttataca gtcgatggtg
    24541 gtcctttagg tgagttgcag tatgaaacgt ttagtgctgc tcaagcaaat attacgatcc
    24601 aagggaaaaa tgtacatcca gggacggcaa aagatacaat gatcaatgct ctacagttag
    24661 caatcgattt tcataatcag cttccagccg atgaagttcc agaaaaaacg gaaggatacg
    24721 aaggtttctt ccatttgatg gcattggatg ggaatcctga agaagcttct atgagttata
    24781 tcattcgcga tcatgatcgt gaaaaattcg aagcacgcaa agcacaaatc acacatatcc
    24841 aagaagagtt gaatcagcga tttgatcaag aacgaatcaa agtagatatg tatgatcaat
    24901 attacaacat gaaagaaatc attgaaaaag atatgagcat cgtggaatta gcgaaacaag
    24961 caatgatcga gcttggtatc tctccagtga tcgaaccagt tcgtggagga acagatggtt
    25021 caaaaatctc ttacatgggt attccgacgc caaatctatt tgccggagga gaaaatatgc
    25081 acggacgatt cgaatttgtt tctttacaag taatggaaaa agcaacagac gtgattgtaa
    25141 aaattgcaga attgaacaca aagagttaaa atcgaaaaag aacagcttat gacaaggcgt
    25201 tcgtcccaat gatggaatgt catctgtcta aggctaaagg gcaggacaat ggaattggtt
    25261 gtcccgccct cttttttgaa gagtaaatag gaggtaaaga atggaaaagc tgctagtttt
    25321 ttataacaaa agttcaggga aagatgaggg tgagaaatta gcacgctggt ttcaagatta
    25381 tgtaaaagaa aagcagccag aattatatgt agaactagct gaaactggcc catcgattga
    25441 tgatgcggaa ttagtcaaga aagcagaagc catagaagct gatactttag taattatagg
    25501 tggagacggc acgattcatc atatcgtcca agcgttccaa aaaacattat ctcaatacca
    25561 tgtcggatta cttccaggtg gaactgtcaa caaccttgca cgtgttcttg agattcccct
    25621 caaaaaagaa gaagctgctg acgtgatcct ggaaggcaat gtacggaaaa tagactatgg
    25681 tcaagtcaat gatgaagtca ttatctctac actgacaatc ggaatcttag cagatacagc
    25741 agtttggatc agtcaagaag aaaaacagcg ttacggctcg tggatcttta tcaagaagtt
    25801 ttttaagtta ttgctgaaga agaaaaaata tcatttggat ttagaaacag acgaggagca
    25861 ttggaaagga aaatcccaac tgttgacagt cacgatgtct aactccgtgg gcggttttac
    25921 aaattttgac gactcagcga ccctagatga tggaaaattt catgtaacga tcgtaccttc
    25981 cttagatatt ttccgattta ttttttattt gcctagaatc atgagaggca aattctatac
    26041 gatccccggc attacttatt tcacagccaa acgcatgaaa atccaagcgc aggaaaaatc
    26101 tgtgggcact cgaacggatg gagacacgac agatgatctt ccagttgaat tatctgttgt
    26161 atcaaaagga ttaacagttt attcaccaaa gaaagaagaa aaagcttaga aatagtagtt
    26221 aacagaaagt tggaagaaga gtaatggatg tattgttaca caatcctttg tggttttacc
    26281 ttttcattgt gaatttttac ttattttgtc tgatggggta tgataaacat caggctagaa
    26341 ctagcggctg gcgtgttcct gagaataatt tgatatttgt cagcctgtta ggcggtggtt
    26401 tgggaggatt gatctctcga aaagttttcc atcataaaac tcggaaaaag aagtttacta
    26461 taggattcgc tgcaggtgta cttgttgatt tgattctcat ttattttttc cattaaggag
    26521 caggagatgt cgtctaaagg aacgaaaact aaaatcattc tgaatctgct tttgctgatt
    26581 gtgatttttg ccattattta ttatttaatc aatcaatcat ttgcagatat ctttgccgaa
    26641 ttgctgtcta ccagtgttgt tgttttggca gcggctgtta tattagggac agtttaccaa
    26701 atcgctgaag ggcgctcaat caaagaaatt gctgaacctt tcgcgccgaa ttttacaacg
    26761 aaagatgggt tcttgacctc tagctatatt gcgttttacc gaatcgtttc gtttggtaca
    26821 ggaacgctga tctcggagat ttatttttat aacaaaaaag gaatgaagct atcccaggga
    26881 gcaggggtga cagcactcca tatgattatg tacaaactgg cagtgatcac ttatgcatta
    26941 gctggcttgg tcatccaatt ctctcttttt tattctaatg gtccatcaat gatctggttt
    27001 atcatcgctg gtatcctttt gacgatcgtg attgttgcct ttttattggc gatgtccgtc
    27061 agcttgaatg ttcaaatatt ttttgtcagc ttatcgaata agttatttaa aagtgagcgt
    27121 ttgcggaaat gggtggatac atgcaatcag cagatctact ctttaaggga aacggtccaa
    27181 accattatcc aagacaggac tgcgctattg cggatttatc tatggaatat gttaaaactt
    27241 gtattttggt atgtacttcc ttatttgatt ctgattgaaa accatcctaa aatagatttt
    27301 ctactgacca tttcgtttat cagctttgca gtagtgcttt caggggttat tcctacgcct
    27361 gctggtattg gttcctttga atttgtctat ttgttattat tcagaccagt tgttggaaca
    27421 gttgacgcag tttcttcgct tttactgtat cgtttcagtt cctttatctt gccatttctc
    27481 tatggatttg tgtatgtgat tgctgaacgg cgaagagtga tcaaagaaga aataatagaa
    27541 gtaaaaaaag agaataaaga aaatcaaggg taggtctttt ataagatcta ccctttttat
    27601 caaatcaaag aaaaacgtta tggataagat aattttacgt ttgtttggct ctttttttat
    27661 atacatacaa tcaattttgt ttttttaaga tttatataag tcgagaaaaa aagtcaaaaa
    27721 actatgtgta tattttgacc aaaattgacc aaagcgatat aatagttttt gtaaagatca
    27781 aagaaataag tgatcttacc tttaattagg agggtatacc tgtatgaata ttgagaaaat
    27841 gactacgacg cttcaagaag cgattgctga agctcaacag attgcggtca ctcgaaaaca
    27901 tcaagatatc gacattgccc atgtatggaa aatcttttta caatctaatc attttggtcg
    27961 aaatttctat acggatgccg gattggatgt cgaatctttt gaacatgaaa ttgatcgtct
    28021 actggatgag tatccagttg tttctggtgg aaacgtccaa tatggacaaa acctcagcca
    28081 gaatttcttt cgattattga atgaagcaga ccaaatacgt gaaagcttcg gcgacgagtt
    28141 tctttcaacg gaagtcgtta ttttagcgtt gatgaaatta aaaaattatc cgttaactgt
    28201 ttatttgaat aaaaacggat tatctgaaaa agaattgcgt aaaaacatcg aagagatgag
    28261 aggaggagaa agagtgacct cacaaaacca agaagaacaa tacaaagcat tagaaaaata
    28321 cggcgttgat ttagtacagc aagtcaaaag cggaaaaatg gatccaatca tcggtcgtga
    28381 tgaagaaatc cgtgatgtca tccgtattct ttcacgaaaa acaaaaaata atcctgttct
    28441 gataggagaa cccggtgtag ggaaaacggc aatcgttgaa gggcttgctc aacgtatcgt
    28501 acgcaaagat gtgcctgaaa atttaaaaga taaaacgatt ttttcattag atatgggtgc
    28561 attgatcgct ggagcaaaat tccgtggtga atttgaagaa agactaaaag cagtcttgaa
    28621 agaagtcaaa aaaagtgatg gacgtatcct cttgttcatc gatgaaatcc acaatatcgt
    28681 aggggcagga aaaacagaag gaagtatgga tgccggaaat ctgttgaaac caatgcttgc
    28741 tcgcggtgaa ttacatttga tcggtgccac aactttagat gagtaccgtc aatatatgga
    28801 aaaagacaaa gcgttggaac gtcgctttca aaaggtatta gtcaaagaac caacagtgga
    28861 agatacgatc agtattctgc gtggattaaa agaacgtttt gagatccacc atggtgttaa
    28921 catccatgac aacgcattag tagcagcagc tactttatct gaccgttata ttactgatcg
    28981 gttcttaccg gacaaagcaa tcgatttgat cgatgaagcc agtgcaacga ttcgggtaga
    29041 aatgaactct atgccaacgg aattagatca agttactaga cgtttgatgc agttggaaat
    29101 cgaagaagct gcactgaaaa aagaatcaga tgatgcaagt aaaaaacgct tgaaaaattt
    29161 gcaagaagaa ttagctgaac ttcgtgaaga agcaaatgcg atgaagatgc agtgggaaac
    29221 agaaaaagaa gaagtcaatt ctgtttcagc aaaacgtgct gaaatcgata aagcaaaaca
    29281 cgaattagaa gatgcagaaa ataactatga tttagaacgc gctgctgtat tgcgtcatgg
    29341 tacgatacct caattagaaa aagaattgaa ggaattagaa gctaaagcaa aagacagcga
    29401 gattaagatg gttcaagagt ccgttaccga aaatgagatt gctcaagtcg ttggcagatt
    29461 aacgggtatc cccgtaacga aacttgtaga aggtgaacga gaaaaactaa ttaaattgaa
    29521 tgaaacactt cataaacgag tgattggtca agatgaagca gttgatgcgg tcagtgacgc
    29581 ggtaattcgt tcacgggctg gattgcaaga cccaaatcgt cctctaggtt ctttcctttt
    29641 cctaggacca actggtgtcg gaaaaaccga attagcgaaa gcgttagctg agaacttgtt
    29701 tgactcagaa gatcatatgg tacggatcga tatgagtgaa tatatggaaa aacatgctgt
    29761 gtctcgtcta gtcggagctc ctccaggcta tgtaggttac gaagaaggtg gtcaattaac
    29821 ggaagctgtc agacgaaatc cttatacaat cgtcttgttg gatgaaatcg aaaaagcgca
    29881 tcccgatgtc ttcaatatct tgttacaagt cctagatgac ggacgcttga ctgattcaaa
    29941 agggcgagta gtagatttca aaaatacggt tctgatcatg accagcaata tcggttcaca
    30001 gttactgctg gaaggcgtga ctgctgatgg aacgattcca gaagcagtgg ctgaacaagt
    30061 caacacgcta ttacgtggaa acttcaaacc agaattcttg aaccgaatcg acgacacgat
    30121 tttatttaca ccattgagcc tagacaacgt gaaaggaatc gttgacaaaa tggtggcgca
    30181 attggcacaa cgtctggaac atcaagaaat cctgctgaca atcagtgatg aagccaaaac
    30241 gtggattgct gaaaatgctt atgaaccagc atatggtgca agaccattga aacgatttat
    30301 cactaaggaa gtagaaacac cactagctaa agaaatcgtt gcaggacatg ttatgcctaa
    30361 atcaaaagta acgattactt tacttgatgg acaacttcat ttcaaaacag aagagctaga
    30421 agaaatcgtc taatcttcca ttgagactat aaaagaaaaa aggggtctga cacaactagt
    30481 gtcagacctc cttttttcaa ataaacagtg ttccagaagt agtttcttcg aaaaataagc
    30541 cgaaatttct tgaagctgcc cgcttctgtc acaacctctt ttattaggta tttttcactt
    30601 tttctaattt ataactttca aagtaacgct catctttttt actttgagac catgcaaaca
    30661 atcccattaa ggcagcacct atcgtatttg tcagcaagtc gcccatcgta tccatcaatg
    30721 ctgctcgtcc gacaaacagt gtgccatcag aagcagcaaa acgttgaaga ttcatgccta
    30781 agaactggtc gcataagaat tcccagaact cccaaaacac gccgcaaagt ccagcaaacg
    30841 caaagccgaa taagaggaac aaccaaggac tggtttttga tatctcagca tctttcatca
    30901 agtaaccaat caatccatag ccaagagctg tcaggaccat tggactcacc gcatgtaaga
    30961 ttttgtccca aaaagagatg atactgatca aatgcatccc tgtgcctaaa aagacagaga
    31021 tgaacaagaa aaaccagtaa aacaaaacga ttgctgctgg tattttaagt ttgaaaactt
    31081 tcaaaatgac ttgtggcatg aaaatcaatg caatccctaa taaagattcc acgataaatg
    31141 tgattccttg agaagtcgaa tatttttgtg tgataaattc ataaaaatta tagcagacaa
    31201 cgaatacacc aaaagcaagt aaagctattt gcaccttttt gatttcttta atcactgaaa
    31261 gtccctccat ctaaaaatct ttttacttct gaaagcaact caatttctga agaaaccact
    31321 tttccgttca atttgatcaa acctattgta tataagttca ggtagtgaaa ttgattttca
    31381 gctgtttctt gtagtgcagc gatttttttc gggtgggttc cgccttgctg tcgagtatca
    31441 gtatataagc cgatcactgg gatgcctttc gcataagcca caccgatttc cgatgctacc
    31501 ccggcatcga tcgtcagccc atctagtaaa gcaatcatta aatcactagc caacactttt
    31561 tctgtgtcag caagagcaat cattcgactg tctgcataag cagttttgtc attgattgct
    31621 tcattttctt gaggaagata gatcgaaagc tcagaagaga gctctctgat ttctttaact
    31681 aactgttggt tatataacaa atcactttta gcaaacattg gtgcggcaaa ataaattttc
    31741 atctaaacat catccttttt atcaaaaata atcgctaaag taagcttcca tgtttggaat
    31801 gatacgctct agtgacttca gtgtttcttc atctgtttct aaccgttcga ttcttgccaa
    31861 ataagaagca cggcgataat tcatcatcaa gcaagtcaat gtttggatat ccagtcgaac
    31921 ggattttcct ataggttcat ttaagacacg gacttgtccc tgttcatccc atgttaaagc
    31981 gaagacacca ttattccaag gagcgacagg atcttcaatg atgaaatgaa aagcatcagc
    32041 tgttccaaca aacggaaacc gttgaaggaa ttcttttaca tcaacgatcc gagccataaa
    32101 atatggttcg atctgctctt tgatctgact atcttcaatc aaaaatgcta aaggctcatt
    32161 tttataaata tctccatgta cccagtagat catggaaaaa tgtgcggaaa taaagttcca
    32221 taagccattt cgagcttctt gatcaagata gaacatttct tttacgtgaa atatttcttc
    32281 agctacccag tagaacagaa cacctcttgg ttcatggttg ctatcgtagt agacagcggc
    32341 tgtgcgctca tcttcatttt cgaaacgcca atactcttcc caatgaaaat cactgcggaa
    32401 taaagcacca tggttttgtt gggcaaattt gtcataaacg gaaaatacgt ctggatgatc
    32461 aactggtaat cgctcaacca taccacttac tgggatttgt ttaggtagct gtgtatctct
    32521 gattttaaag gaaagtttat cagacatgat ttcccatcct ttacgccgat agtaggggat
    32581 actgtaagga tacaaataag agatccattg cttattcttg cgcatattat ccaatgcaac
    32641 gataattaga tcttgcatca atccatggtt agcatattct ggataagttc caactccagt
    32701 cacaccaccc attttataac gtgcaccgtg aatattgact tcacaaggat agatagcgat
    32761 ttgagagatc agtttatttt cgtggaacca tccgaacact ttcgatactt ccaaaatcgg
    32821 ttttttggaa cggataaaag ctcgtttatt ctcaaacccg ctttcctcga tgtctgcttc
    32881 agtcacttgg aacacatagg aaagcaattc attgaattga tcgatatgtt cttgagtgac
    32941 cggttttaat tcaagatttt ctctaaaatc tttttgatcc attttttcat ctccatttat
    33001 tagcttgata acagtatagc aaaattctac aaaaaaaact tctatagctg actaattaaa
    33061 aactctttaa attactgctt ttcgttggta taatggaaag tgctgattat atgaaaagag
    33121 gagacgctat ggatataaag aaaatgaaag aacgccaaga gcagattcgt aatttttcga
    33181 tcatcgctca tatcgaccat gggaaatcta cattggccga tcgtatttta gaaatgacgc
    33241 acactgtgac gtctcgcgaa atgcaagatc agctgttaga ctcgatggat ttggaacgtg
    33301 aacgtggaat cacgattaaa ttaaatgctg tagaactcaa ttacacagcg aaaaatggag
    33361 aaacatatat ctttcacttg attgacaccc cagggcacgt cgacttcacc tatgaagtct
    33421 ctcgaagcct tgctgcctgt gagggtgccg ttttagtggt cgatgcagca caaggaatcg
    33481 aagcacaaac attggccaat gtttatttag cgttagacaa tgatttggag atcctgccag
    33541 tcatcaataa aatcgaccta cccgcagcag atcccgaacg tgtccgtcaa gaaatcgaag
    33601 acgtaatcgg aatcgatgct agtgaagctg tattagccag tgccaaatca gggattggga
    33661 ttgaagatat tctcgagcag atcgtggaat atgttcctgc accaagtgga gacttagatg
    33721 caccgctaaa agctttgatc tttgactcta tttatgatag ttaccgcggt gttgttctaa
    33781 atgtccggat cacggacggt atggtcaaac ccggagacaa aatcaaactg atgagtaatg
    33841 gcaaaacatt tgatgttaca gaggtcgggg tcttctcgcc aaaagcagtt gctagagact
    33901 ttttgatggt cggtgatgtc ggttatatca cagcaagtat caaaactgtc caagatacac
    33961 gagtagggga tacagtaacg ttagcagaca atcctgccgc tgaagcttta ccaggttacc
    34021 gaaaaatgaa tccgatggtt tattgtggtc tttatccaat tgatacttct cgttacaacg
    34081 atttgcggga agctttagaa aaactgcaat tgaatgatgc tgctttacaa ttcgaaccag
    34141 aaacctctca agcattaggt tttgggttcc gctgtggatt cctcggactt cttcatatgg
    34201 atgtcgtcca agaacgcttg gaacgggaat tcaatctaga attgatcacg actgcaccat
    34261 ccgttattta ccatgttaat aaaacggatg gtacgactgc tacagtagat aacccggcag
    34321 attttcctga acctgtaacg attcaggatg tagaagaacc attcgtcaaa gcacagatca
    34381 tggtcccaaa tgattttgtc ggtgcagtaa tggaactttc tcaacgaaaa cggggagaat
    34441 tcattacgat ggattattta gatgattacc gagtaaatgt cgtctataat attccgcttt
    34501 ctgaaattgt ctttgacttt ttcgacaaat tgaaatcaag cacgaaaggc tatgcctcat
    34561 tggattatga aatgtctggt tatcaaaaaa gcaagttagt gaagatggac atcttgctga
    34621 atggagaaaa agtcgatgca ttaagcttta tcgttcaccg agactttgcc tatgaacgtg
    34681 gaaaagcaat cgttgaaaaa ctgaaaaaac taatcccacg ccaacaattt gaggtgccaa
    34741 tccaagcagc tattggacaa aaaatcgttg cccgttcaga catcaaagcc ttgcgtaaaa
    34801 acgtcttggc taaatgttat ggtggagatg tttctcgtaa acgtaaactc ttagagaaac
    34861 aaaaagaagg gaagaagcgg atgaaacaaa tcggctccgt cgaagttccg caagaagcct
    34921 tcatggccgt tctgaaaatg gacgaggacg aaccgaagaa atagcaaggg tttgtttgaa
    34981 aacagtgtaa aaaataaacc atgtttacat ggatttgcca ccggtttgcc accggtggtt
    35041 tttttcggtg gcaaaaatca agaattcttt ctaagatttt ccatgatatc gaccgtttca
    35101 tttctcatct tatctgttac atgcgagtaa gtatccatcg taattgaaat tctgctgtgg
    35161 cccaatcgtt cagagatttc tttcattttt gcgccatttt cgagaagaag tgtagcatga
    35221 gtatgtctga gagaatggaa gttaaaagag agggagagtg cattcgatat cctcctagta
    35281 ttccatttca ctacacttgg cgtaactagc tcaccgtctt ccttcgtaca tactgcattt
    35341 gaatcaatgt agagctttcc atacttcatt cgattttcta attgttgttt cttatgtttt
    35401 ttcagaattg ctagcaaggt ttgtccaata aaaatcgttc gattggaact gcttgtcttt
    35461 ggcgtaccat atacccatgc gccgtcattc tttaccattt gtttctctac agtaattgtt
    35521 ccatttgaaa aatcgacatc atcccacgtc agaccacaaa cttcgccaac gcgcattccc
    35581 gtataaaatc caatattcaa aggaatatag aaaggatgtc cttcaggagt aatttctagc
    35641 atatgatcaa agtcctcaag agaaatgatt tttagatctt ttttagtcgt tggtcgtgct
    35701 tcatattttg gtatttttac atacagcata ggattttgct tgattaatcc ccaaggataa
    35761 accgccatat tcagcgcatt cttaaggaca gagtgagtaa tagtcattgt tttcttcgag
    35821 taaccctttt taaattcagc attgatgaaa ttttgtaaaa gagcagggga gagatccgta
    35881 agttttttct ttcctaaata accgtttata tgatttttga tggtaaatcg gtagttttca
    35941 taggtattgt attttagatt tagtttaacg tattcctcca tccaaaaatc aaggtattgt
    36001 tttactcgag tatccgtacc taaaaagtat tgtcctgttt cgtcaatatc tgataaaact
    36061 tttcgtaaag cagcttcggc ctctggtcgg gtgtctccgc caactttctc cacttttttt
    36121 cttgagccat catcattgat atcttcgccg tttgtataat taagcgagac aaataaaaaa
    36181 gccatttatg ttacactc
//
LOCUS       DAZKLM010000019        34962 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3764
            SAMEA115706913-rid22582563.denovo.019, whole genome shotgun
            sequence.
ACCESSION   DAZKLM010000019 DAZKLM010000000
VERSION     DAZKLM010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706913
            Sequence Read Archive: ERR13196647
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34962)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34962)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287823.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287823.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,004
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,849
            CDSs (with protein)               :: 2,849
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 41 of 96
            Pseudo Genes (incomplete)         :: 52 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 15 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34962
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706913-rid22582563.denovo.019"
                     /isolate="VRE3764"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            157..1863
                     /gene="efbA"
                     /locus_tag="ACG842_000862"
     CDS             157..1863
                     /gene="efbA"
                     /locus_tag="ACG842_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin-binding protein EfbA"
                     /protein_id="HFQ0247424.1"
                     /translation="MSFDGVFTHAMVNELRETLLSGRISKIHQPYENEIVLVIRSRGK
                     NHRLLLSAHPSYARVQITQIDYQNPDNPPNFVMMLRKYLDGAILEDIEQIENDRVIHF
                     HFAKRNELGDLQNIILIVELMGRHSTIVLVNRETGKILDAIKHIGSSQNTYRSLLPGV
                     EYVAPPKQEVLNPFSSEKEKIFQRLSQTELDPKAIQRQFQGIGFDIAQELTKRLLERP
                     NEKMVVWDEFFSAISHQPIPTFYETENKDFFTPIAYQVLSEQASAVTAYPTLSQLLDS
                     YYHEKAEKDRAKQQGGELIRKIENELKRNKNKLKKREQTLKESENAENYRRNGELLTT
                     FLTQVPRGAKEVVLPNYYEEDRPIKIALDPALTPNQNAQKYFHRYQKLKNAVKLIGEQ
                     IQEAKDEIQYLESVLSQLEIAGPMDIEAIKEELTSEGYLKKKNLKKQKRKKPSQPDQY
                     FSSDGTLILVGKNNLQNDQLTMKTAKKTDYWLHAKNIPGSHVIIKSDKPSDETITEAA
                     ELAAYFSKYRYSAQVPVDLVQVKHIRKPNGAKPGYVIYENQKTIIVTPEEEKITAMKQ
                     NR"
     gene            complement(2021..2923)
                     /locus_tag="ACG842_000863"
     CDS             complement(2021..2923)
                     /locus_tag="ACG842_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFQ0247425.1"
                     /translation="MNIQQMKYVVAVANNGSFREAAKKMFVSQPSLSAGIKELETELG
                     ITLFTRTNRGAYLTEEGQDFLNRAEKILVQLDTLETHYLTSEKRDNFSIAAQHYDFLG
                     PLTAQLLEEFKDEIKQFRIMETTTARVIEEVKEQHSEIGIIYMNEHNQAGIRRYLQQG
                     ELSGRSIGKFKTHIFLRQGHPLAEHAEITKEELLSYPQVRFTQDGNNFPYFYEDLIEI
                     PDQETVIYTSDRGTLMNIVLETDAYASGSGIVIGGIREHLRLIPLAEGELNEFYIIYS
                     AKRPLSEIAQRFIKELTRLLDQQS"
     gene            complement(2920..3612)
                     /locus_tag="ACG842_000864"
     CDS             complement(2920..3612)
                     /locus_tag="ACG842_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase family protein"
                     /protein_id="HFQ0247426.1"
                     /translation="MDVEWGYSGDKGPENWASLCDWFARGAEFPLQSPIHLTKGEETK
                     IEGDTLSFHYQKQRFTDKEFKNTIHLVPFDQLSYVEFKKERYYLTDIHFHLPSEHIIN
                     GKQEDIEFHFVHMNSKKENLVVGVMYQLMVQEGWLYNQENGESWNLEKHEHWVNPDVF
                     FPEQKSHFHYIGSLTTPPTSGPIQWFVMDHVGKLNQEFLLPFDGQYARPNNRPIQPLN
                     GREIYYFEEFEQ"
     gene            complement(3655..4296)
                     /locus_tag="ACG842_000865"
     CDS             complement(3655..4296)
                     /locus_tag="ACG842_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbR-like 5'-deoxynucleotidase"
                     /protein_id="HFQ0247427.1"
                     /translation="MGLNEFILGLNNLETITRAPGFFKYTEHTVAAHSYRVASIAQVL
                     GDIEEANGQPIDWKLLYEKSLNHDYTERFIGDIKTPVKYASKELRGMLQTVEEKMTDE
                     FINQEIPAEFQEVYRRRLFEGKDETVEGELLAISDKVDLLYESFDEIIKNNPEKVYKE
                     MFIEAVTTIKSFEHRPSVQYFFKEIFPELLNQEFYGKEAFLETVSTIVKKEEK"
     gene            complement(4334..4648)
                     /locus_tag="ACG842_000866"
     CDS             complement(4334..4648)
                     /locus_tag="ACG842_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0247428.1"
                     /translation="MADLSALDEDREIRFVSPESSIGIAKIQKLIARSIFSSGMKRNF
                     YRDQFLIFLKFIFLRLVSRKKRIISSLFSIIYRFSLFFGKGAVSKMVKSGNVANISYL
                     MI"
     gene            complement(4636..5265)
                     /gene="pyrE"
                     /locus_tag="ACG842_000867"
     CDS             complement(4636..5265)
                     /gene="pyrE"
                     /locus_tag="ACG842_000867"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HFQ0247429.1"
                     /translation="MNVEQSIAKDLLEIEAVFLNPSNPFTWASGIKSPIYCDNRITMS
                     YPKVRKEIAKGLASKIKEAYPEVQVIAGTATAGIPHAAWVAEILDLPMVYIRSKAKDH
                     GKGNQIEGRIFEGQKMVVIEDLISTGGSVLEAAEAAKREGADILGVAAIFTYELPKGK
                     ANFEKAEIPLMTLTNYSVLIEAALEDRYIDEQELTLLKEWKKDPENWQI"
     gene            complement(5272..5988)
                     /gene="pyrF"
                     /locus_tag="ACG842_000868"
     CDS             complement(5272..5988)
                     /gene="pyrF"
                     /locus_tag="ACG842_000868"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HFQ0247430.1"
                     /translation="MNQLDQRPIIALDFSTRQEVEDFLRFFPKEEKLFVKIGMELFYQ
                     EGPEIVRYLKEAGHNVFLDLKLHDIPNTVEKAMRGLAKLGVDLTCVHAAGGIRMMEAA
                     MRGLEEGTPEGGKRPLLLAITQLTSTSEEEMHADQLIEVSLEKSVIHYASCAKKAGLD
                     GVVSSAWEVEAIKEMAGDEFVCLTPGIRPEGTVAGDQTRVVTPSQAKKIGSTFIVIGR
                     PITQSTNPYKAYQTIQTEWS"
     gene            complement(5985..6917)
                     /locus_tag="ACG842_000869"
     CDS             complement(5985..6917)
                     /locus_tag="ACG842_000869"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HFQ0247431.1"
                     /translation="MNENPLAVKLPGLELKNPIMPASGCFGFGKEYGKYYDLNQLGSI
                     MVKATTPNARFGNPTPRVAETPSGMLNAIGLQNPGLDVVMHKLLPELEEYNELPIIAN
                     VAGACEEDYVEVCSKIGEAPNVKAIELNISCPNVKHGGIAFGTDSDVAFQLTQAVKKV
                     SSVPVYVKLSPNVTDIVPIAQAIEAGGADGFTMINTLLGMRIDLKTRKPILANQTGGL
                     SGPAIKPVAIRLIHQVASISSLPIIGMGGVQTVDDVLEMFMAGASAVAIGTANFTDPY
                     ICPKLIKELPVRMSELGIESLERLIKEVREERER"
     gene            complement(6914..7690)
                     /locus_tag="ACG842_000870"
     CDS             complement(6914..7690)
                     /locus_tag="ACG842_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HFQ0247432.1"
                     /translation="MKQEMMEIVQQKQLAPKIFEMTLKGDLVKEMQQPGQFLHILVPR
                     ADLLLRRPISLNHIDHETNTCRIIYRVEGEGTRVFSTLSAGDHLDVMGPLGNGFDLSN
                     LHTGDEVFIIGGGIGVPPLYELSKQLVEKGIKPTHFLGFATHEVVYYEKEFNRLGETR
                     IATDDGTYGMHGNVGNLLLGTKTEPTAVFACGSNGLLKTVEQLFSSHQNVQLSLESRM
                     ACGIGACYACVCHTPEDETGTKSVKVCDEGPIFKIGEVII"
     gene            complement(7744..10929)
                     /gene="carB"
                     /locus_tag="ACG842_000871"
     CDS             complement(7744..10929)
                     /gene="carB"
                     /locus_tag="ACG842_000871"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HFQ0247433.1"
                     /translation="MPKRTDIKKIMVIGSGPIIIGQAAEFDYAGTQACLALKEEGYEV
                     VLVNSNPATIMTDKEVADQVYIEPITLEFVSRILRKERPDALLPTLGGQTGLNMAMEL
                     AASGILEELNIELLGTKLSAIDQAEDRDLFKQLMKELNQPIPESEIVNTVEEAVDFAN
                     KIGYPVIVRPAFTLGGTGGGMCENEEELRIVSENGLSLSPATQCLIERSIAGFKEIEY
                     EVMRDSADNAIVVCNMENFDPVGIHTGDSIVFAPSQTLSDHEYQMLRDASLSIIRALK
                     IEGGCNVQLALDPHSFNYYVIEVNPRVSRSSALASKATGYPIAKLAAKIALGLTLDEM
                     KNPVTGTTFAEFEPALDYVVAKIPRWPFDKFENGERLLGTQMKATGEVMAIGRNIEES
                     LLKAVRSLEIGTHHIELPELADITDDDLIQKVVRAQDDRLFYVAEAIRRGYTIEELAD
                     LTKIDLFFLDKLLHIVEIEQQLKQQPLDDKILKTAKQNGFTDKKVAELWEINEEAVRQ
                     MRKENDLIPVYKMVDTCAAEFESKTPYFYSSYEYENESSRSEKPSILVLGSGPIRIGQ
                     GVEFDYATVHSVKAIQQAGYEAIIMNSNPETVSTDFSISDKLYFEPLTFEDVMNVIEL
                     EQPIGVIVQFGGQTAINLAEPLTAAGVKILGTTIEDLDRAENRDLFEQALKELDIPQP
                     LGDTATSKEEAVVIASRIGYPVLVRPSYVLGGRAMEIVENQRDLEDYMEHAVKASPEH
                     PVLVDRYLLGGECEVDAICDGETVLIPGIMEHIERAGVHSGDSMAVYPPQTLSESIKK
                     TIEDYTIRLALGLNCVGMMNIQFVIHENEVYVIEVNPRASRTVPFLSKVTGIPMAQIA
                     TKAILGEKLKTLGYENGLYPETKEVHVKAPVFSFTKLQKVDTYLGPEMKSTGEVMGSD
                     RQLDKALYKAFEASGLHLPEYGAVLFTIADETKEEALELAKRFTEIGYSLIGTKHTAA
                     YFEKNGLVVETVAKISEEAAEKNVVDLIRDGKTQVVVNTIDKDRGNASKDGFIIRREA
                     VEHGIPLFTSLDTADAIVRVMESRAFSTNPI"
     gene            complement(10934..12010)
                     /locus_tag="ACG842_000872"
     CDS             complement(10934..12010)
                     /locus_tag="ACG842_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl phosphate synthase small subunit"
                     /protein_id="HFQ0247434.1"
                     /translation="MERLLILEDGTVFEGKAFGADASVFGEIVFTTSMTGYQETITDQ
                     SFNGQIITFTYPMVGNYGVNRDDYESISPTCKGVVVKEHARVASNWRNQMTLDSFLKQ
                     KGIPGISGIDTRALTRKIREVGTMKASIVDAGDSFEHAFDQLKATVLATNQVQQVSTN
                     KPYPSPGTGRKVIVIDFGLKHSILRELSKRNCNLTVLPYNTDAETILALSPDGVMLTN
                     GPGDPKDVPEAIEMIKKIQGKVPIFGICLGHQLFALANGADTYKMKFGHRGLNHPVRE
                     VATGRIDFTSQNHGYAVDEATIDPEKLIVTHVEVNDGTVEGVKHRDYPAFSVQFHPDA
                     APGPHDALHLFDEFMEMMDAGKEQ"
     gene            complement(12015..13295)
                     /locus_tag="ACG842_000873"
     CDS             complement(12015..13295)
                     /locus_tag="ACG842_000873"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HFQ0247435.1"
                     /translation="MKTLIKNGQINTRKNMTTPAEIWIEDGRIKAIGTGFSEAEFDEV
                     FDAKGQLITPGLVDVHVHLREPGFTYKETIEAGTRSAARGGFTTVCAMPNLNPVPDTA
                     EKLRQVYDIIRKDAVVKVLQYAPITENLRSEKLVDQEALIEEGAFAFTNDGVGVQTAG
                     TMYLAMKEAAKNNKALVAHTEDESLLFGGVMHAGKKAEELGLPGILSVTESSQIARDL
                     LLAEATGVHYHVCHVSTKESVRVIRDAKKAGIHVTAEVSPHHLILIDEDIPEDFGFWK
                     MNPPLRGREDREALIEGLLDGTIDCIATDHAPHGLEEKSQSFMKSPFGIVGSETAFQL
                     IYTHFVETGRFTLEQVINWLAVKPAEIFGLNAGTLTVGAPADVAVFDITQTCTIDKED
                     FLSKGENTPFIGWKVKGETQMTFVNGKLVWQKGE"
     gene            complement(13314..14240)
                     /locus_tag="ACG842_000874"
     CDS             complement(13314..14240)
                     /locus_tag="ACG842_000874"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HFQ0247436.1"
                     /translation="MIIKSERISLKHLLTVEALTDQEVMGLIRRGQEFKQGANWTPQK
                     RQYFATNLFFENSTRTHKSFDVAEKKLGLEVIEFEASTSSVQKGETLYDTVLTMSALG
                     VDVAVIRHGDENYYDELIQSKTIQCSIINGGDGSGQHPTQCLLDLMTIYEEFGHFEGL
                     KVAIVGDITHSRVAKSNMQMLKRLGAQVFFSGPREWYDDEFEVYGHYMPLDELLDQVD
                     VMMMLRVQHERHDGKESFSKEGYHQEHGLTVERAKKLQKHAIIMHPAPVNRDVELADS
                     LVEGLQSRIVQQMSNGVFVRMAILEAVLNGKA"
     gene            complement(14253..15536)
                     /locus_tag="ACG842_000875"
     CDS             complement(14253..15536)
                     /locus_tag="ACG842_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="solute carrier family 23 protein"
                     /protein_id="HFQ0247437.1"
                     /translation="MSEKKGFHNDDVVLDIHDRPTPGHWVGLSLQHLFTMFGATVLVP
                     ILVGIDPGIALVSSGLGTLVYLFTTKGKIPAYLGSSFAFIAAMQLLMKTDGYPAIAQG
                     AVTTGLVYLIVALIIKKIGSAWLDKILPPIVVGPVIMVIGLGLAANAATNAMYNQGTY
                     DFKYIAVALITLGLTIFFNMWLRGFLGLIPILLGIISGYIVALLFGIVDTKPIVDAAW
                     FAVPNFEIPFVQYEPKLYLGAITTMAPIAFVTMTEHIGHLMVLNKLTKRNFFEEPGLH
                     KTLTGDGLAQIVAAFVGGPPVTSYGENIGVLAITRVHSVFVIGGAAVFAVILGFVGKL
                     SALILSIPGPVISGISFILFGVIAASGMKILIENKIDFDKKKNLLIASVILVVGIGGL
                     VFEVGTFTLSAMALATVLGIILNLILPETSRSEEN"
     gene            complement(15550..16089)
                     /gene="pyrR"
                     /locus_tag="ACG842_000876"
     CDS             complement(15550..16089)
                     /gene="pyrR"
                     /locus_tag="ACG842_000876"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HFQ0247438.1"
                     /translation="MQAKEVVDQVTMKRALTRITYEIIERNHSIQDIVLVGIKTRGIY
                     IASRIAERLKQLEDIDIPVGELDITLYRDDKKENPEEPELHSSDIPVSLEGKEVILID
                     DVLYTGRTIRAAMDAVMDFGRPRKISLAVLVDRGHRELPIRADYVGKNIPTSRAEEIL
                     VEMQELDGQDRIMILKEED"
     gene            complement(16324..17235)
                     /locus_tag="ACG842_000877"
     CDS             complement(16324..17235)
                     /locus_tag="ACG842_000877"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HFQ0247439.1"
                     /translation="MTELKTTIHHESGRIDKVLTNLFTDYSRSQIQLWLKNGAVSVNG
                     QPVKANYKVKQGDEITVLIPEPETLDVVPENIPLTIVYEDEDVAVINKPQGMVVHPSA
                     GHTHGTMVNALMYHIKDLSTINGVIRPGIVHRIDKDTSGLLMVAKNDQAHESLAQQLK
                     DKTSLRKYVALVHGEIPHEKGTINAPIGRSKENRKMQAIREDGKSAVTHFTVLERFEG
                     FTLVELKLETGRTHQIRVHMKYIGYPLAGDPVYGPKKTLKGNGQFLHAQMLGFTHPTT
                     NEKMIFEAPLPEVFEKTLEKLRKNIAF"
     gene            complement(17232..17696)
                     /gene="lspA"
                     /locus_tag="ACG842_000878"
     CDS             complement(17232..17696)
                     /gene="lspA"
                     /locus_tag="ACG842_000878"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HFQ0247440.1"
                     /translation="MLIIYWIFSALLVGVDQLVKWWITDNLALGETKNLIPGILSLNH
                     IRNTGAAWSMLEGKMWFFTIVTLIAVVVILTLMIKNREKGNRWFMIGLSLILAGAIGN
                     FIDRIRLGYVVDMFQTDFMSFPIFNVADVTLVCGVICMLIYIILDEKDQRKK"
     gene            complement(17701..18399)
                     /locus_tag="ACG842_000879"
     CDS             complement(17701..18399)
                     /locus_tag="ACG842_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HFQ0247441.1"
                     /translation="MANSDLFLISFNRIEKWMRDEMGNARNMGFTELVRRLAQRKQLM
                     IRKYEDDLLQLAQLRNAIVHDRIAVDFVIAEPNDWTVKRIQLIEQELIQPETVLPRFA
                     KRVTGFEQDLPLLELLKIVAEKRYSQFPLYNKGKFVALITLRNIGFWLAKESQKGPVD
                     LTNKRALDLIIQNGKYTNYHFVPAKTHIYEVEAMFREQGTLEAVLITKDGNPDGNLLG
                     IVRPRDIYKQIEKD"
     gene            complement(18386..18772)
                     /locus_tag="ACG842_000880"
     CDS             complement(18386..18772)
                     /locus_tag="ACG842_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0247442.1"
                     /translation="MKTTFIRKTGFYGMGSSIQLRINNEKRSVLSHEQKITLDLEVPF
                     TMQVTFYWLKSPVYTVKEAKPAYVITMNYLILQIYPFLFLATGLGAVFLQNLFFSFFA
                     VIVLIAFFFYIKNRAYLIKEENYGKL"
     gene            complement(18919..20589)
                     /locus_tag="ACG842_000881"
     CDS             complement(18919..20589)
                     /locus_tag="ACG842_000881"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HFQ0247443.1"
                     /translation="MKNDLQISQETELEPIVEVASKLDLKEDDLELYGKYKAKINFSG
                     INRSKDNAEGHLILVTSINPTPAGEGKSTITVGLGDALNKINKKSVIALREPSLGPVM
                     GIKGGAAGGGYAQVLPMEDINLHFTGDMHAITTANNALSALLDNHIHQGNELNIDARR
                     VIWKRVVDLNDRELRKVIVGLGGPIQGVPREDGFDITVASEIMAILCLAADLEDLKAR
                     LARIVVGYTFDRQPVTAGDLKAEGALALLLKDAIKPNLVQTIYGTPAFVHGGPFANIA
                     HGCNSILATKTALRLADYVVTEAGFGADLGGEKFLDIKVPNLKKAPDAVVIVATVRAL
                     KMHGGMKKDELKNENLDALKVGFANLKRHIRNMEQYQLPVIVAINEFVTDTDNELTLL
                     EHLCENQGILAKRASVWADGAEGGVDLAKAVVRLIDRKEANYKPLYRLEETIQEKTET
                     IVKKIYGGNGVVFSKKAQKQIEEFTKNGWDKLPICMAKTQYSFSDDPSLLGAPEDFTI
                     TIREIIPKLGAGFLVALTGDVMTMPGLPKKPAALNMDVTNDGEVLGLF"
     gene            20772..21005
                     /locus_tag="ACG842_000882"
     CDS             20772..21005
                     /locus_tag="ACG842_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFQ0247444.1"
                     /translation="MTTGIVKWFDNKKGYGFISYDDTEEIFVHFTAIEEEGFKTLEEN
                     QVVEFEIIEGNRGTQAAHVKKGNVQTTDTSMHE"
     gene            complement(21104..21535)
                     /locus_tag="ACG842_000883"
     CDS             complement(21104..21535)
                     /locus_tag="ACG842_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EbsA family protein"
                     /protein_id="HFQ0247445.1"
                     /translation="MKKNIRWQPELAHTIIYWSLTLIILFFSLILSLENTGPYWKSNF
                     VLGIFLVFLFLGLRRSFILKSNSVIIRYAAFWKDREISVGQIRSLTPTNTGVQMMLQD
                     EKAPSVFMMRKKDQVVLFDHFEKNGPKSESIPYPYTKKRKS"
     gene            21614..22027
                     /locus_tag="ACG842_000884"
     CDS             21614..22027
                     /locus_tag="ACG842_000884"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HFQ0247446.1"
                     /translation="MLKVYIDASTKGNPGPSGGGILIVYQGKQEQLSIPLSIGTNHQA
                     EFEVFLKTLEFLKKNDLQNETIFCFSDSKTLVSTVDKNATKNELFLPYLQRIQELLSE
                     FSLLILQWLPENKNKGADNLARQALHKQLKEKKTL"
     gene            complement(22065..23213)
                     /locus_tag="ACG842_000885"
     CDS             complement(22065..23213)
                     /locus_tag="ACG842_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HFQ0247447.1"
                     /translation="MAKKQEEKKKQIPQQRTKKGVLAGILGLVTNLILFVSKLMIGLF
                     SGSVSIMADAVNSLSDTASSILTLIGFKIAAKPADQEHPYGHERFEYISGLFVSIIIT
                     YVGFQFLESSAEKIIHPENVSLTPIVFFVLIISIFLKFAQGKMYQVIAQSIDSETLRA
                     TATDSYNDVFTTLAVLLSAAIEWVTGWRIDGYIGFALALYILYSGLTMLRNFVYELLG
                     SRPTPAEIEEMEEHLNACDDILGYHDLLVHNYGPNKRFASVHIEVDESMNLNVAHKII
                     DLIEKDFKKSLDVELVCHLDPMPVDNEKYYNILRQLKQILLETGTGLNIHDFRVIHQG
                     KTLQFDVVVPEKFQFVDTELERILRSQIEEKIGDYQLEITFDHNYLLY"
     gene            complement(23335..23931)
                     /locus_tag="ACG842_000886"
     CDS             complement(23335..23931)
                     /locus_tag="ACG842_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFQ0247448.1"
                     /translation="MNSRNKTFRLVLRAILLAIIIVQAMVPWLGFIPLGFISLTIIHI
                     TVIIAAVVLGPKDGMVIGLFWGIATIVRAYAMPTTPFDTLVFTNPIISVVPRVLVGLV
                     AGLVFHWIYQKNKSITISSAFAGVLGSLVNTVLVLGFMGLFYTGATADAYGVDSALLF
                     KTLAGVAAINGIPEAIGAGIITPLLAKALFAATPLKPE"
     gene            complement(24131..26773)
                     /gene="alaS"
                     /locus_tag="ACG842_000887"
     CDS             complement(24131..26773)
                     /gene="alaS"
                     /locus_tag="ACG842_000887"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HFQ0247449.1"
                     /translation="MKQLSSAQVRQMFLDFFQSKGHTIEPSASLIPVNDPTLLWINSG
                     VATLKKYFDGSVVPENPRITNAQKSIRTNDIENVGKTARHHTMFEMLGNFSIGDYFKN
                     EAIHWAWEFLTDPKWMAFDPEKLYVTVYPKDTEAKRIWHEEVGLPLDHIIDVEDNFWD
                     IGAGPCGPDTEIFYDRGEAYNDLAEDDPENYPGGENERYLEIWNLVFSEFNHKPDNTF
                     EPLPHKNIDTGMGLERMVSIVQDASTNFETDLFMPIIHAVEKLSKQVKYGENPVTDIS
                     YKVIADHIRALSFAIGDGALPSNEGRGYVLRRLLRRAVMHGKKLGIEQAFLYRLVPVV
                     GEIMVSYYPEVLQQKEFIEKVIRTEEERFNETINEGLEILNQVIQEVKNENGTTLAGK
                     DIFKLYDTYGFPVELTEEVAEDEGLKVDHAGFEKEMDAQRERARSARSTEKSMGVQSA
                     LLTDIKVESKFIGYTEQESDSQLLVIVKGESLLNEVAEGEAQMIFDQTPFYAEMGGQV
                     ADQGWIMNEVGEIVAVVTDVQKAPNGQFMHTVEVKAPMSEGQTYHLLIDQSMRNRIIK
                     NHTATHLLHKALKEVLGEHANQAGSLVAPGHLRFDFTHFGQITSDELAEMERIVNEKI
                     WEAIPVETIETDIDTAKNMGAMALFGEKYGHDVRVVNIGGWSIELCGGTHVANTENIG
                     IFKIVSESGIGAGVRRIEAVTSKEAYELLEEEEKQLKQVATIVKSPQLKEVVSKAEQL
                     QQQVRELQKENEQLASKLANQQAGDVFKDPETINGKTIIAAQVKVKDMNQLRQLADQW
                     KQKNISDILVLANEQEGKVSLLAAMTKESNEAGLKAGDLIKAIAPKVGGGGGGRPDMA
                     QAGGKNPAGIPDALKAAKEWVAQQ"
     gene            27161..27592
                     /locus_tag="ACG842_000888"
     CDS             27161..27592
                     /locus_tag="ACG842_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0247450.1"
                     /translation="MKKIVGADEWIRAASCFLRYQVFVLEQGIPAELEFDGKDIPQQT
                     YYLFLDQKRPVATLRYEKKTPTCFKPERFCVAKEYRGKGLGGQLLEAAEKRAVSEGCQ
                     TSYVVAEMTAVDFYEKQGYHTCTEPFIEDEVLCIGMKKSLA"
     gene            complement(27637..28986)
                     /locus_tag="ACG842_000889"
     CDS             complement(27637..28986)
                     /locus_tag="ACG842_000889"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFQ0247451.1"
                     /translation="MNNFDIFRFKPFINEALTAKGFKQPTEVQERLIPLIAKGKNIIG
                     QSQTGTGKTHTFLLPLFEKINAEKEEVQVVITAPSRELAAQIYEAATQIAKFSTPEIR
                     VSNFVGGTDKQRQLNRLHHQQPQVVVGTPGRILDMMNEQALKIHTAFAFVVDEADMTL
                     DMGFLNEVDQIASRLPEKLQFLVFSATIPEKLRPFLRKYMENPIIEEIKPKAVISEDI
                     ENWLISTKGKDENQMIYQLLTIGHPYLAIVFANTKQHVDEIADYLKNQGLKVAKIHGD
                     ITPRERKRVMRQVQNLEYQYVVATDLAARGIDIEGVSHVINAEIPEDLDFFIHRVGRT
                     GRNGLKGTAITLYSPNDEQAIDAIEKLGVSFEPKEIKNGEIVKTYDRNRRAKREKSKD
                     VLDPTLIGLVKKKKKKIKPGYKKKIDWAIAEKNKKERKIERRQQSRTARKNKKNSHK"
     gene            complement(29056..30219)
                     /locus_tag="ACG842_000890"
     CDS             complement(29056..30219)
                     /locus_tag="ACG842_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HFQ0247452.1"
                     /translation="MASYIEPIRFALIVFPFLALAISSVFFIYEYRKYGTFLVTRAVI
                     LYSFVFYLLCAYFLVILPLPAKETVAQMTGPKMELHLFASWQHFRDNTVLVLSEPSTY
                     LPAMKQSVFLEPAFNVLLLIPFGIYLRYYFRLSFMKTMIASFCLSLFFELTQLSGLYF
                     IYPRPYRLFDVNDLVTNTLGGIIGFVVTPLFTFMLPTRRRMDELSYEKGKTVSLTRRL
                     VAYLIDWTFLSLINLVAAIFFKIAFDSGAVTSHIWWLLLEVGVYFMIVPFITNGWTLG
                     KKIVRIRLVEEDRERISLKALMIRYGYLYFLFYGLSWLGQLLQQIMNSTTNTVLQMSA
                     VFISLGVLLLQGIFILNILLSFLRKKRILFYEKASHTYTVSTIGQTKDDDSRN"
     gene            30348..30671
                     /locus_tag="ACG842_000891"
     CDS             30348..30671
                     /locus_tag="ACG842_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0247453.1"
                     /translation="MLNMYFVFGVPIFLLFLYATIAYVRKRTTIHYLGFILLIISGFM
                     LVFNLQTWQQALLEMDKMTPHALSKVLGYPVYLIWLPIFISGCLVLLNIYRGVRRIVQ
                     LRKSK"
     gene            complement(30854..32203)
                     /locus_tag="ACG842_000892"
     CDS             complement(30854..32203)
                     /locus_tag="ACG842_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFQ0247454.1"
                     /translation="MEEYQATPVKEVKIENELKRTMGFFTALSTVMGTVIGAGVFFKA
                     ASVAEVTGSASLHMFSWFLGGMISVCAGLTGAELAAAIPETGGMIKYIERIYGNTAAF
                     LLGWAQVVIYFPANVAALSIIFGTQFVNLFGLSQSMIVPVAVTAAVSILLINFLGSKA
                     GGAFQSITLVCKLIPLFVIVIFGLFRQEGVDFQLFPIQAGENLSFFSALGAGLLATMF
                     AYDGWIHVGNISGELKKPAKDLPKAISLGIIGIMIVYLLVNAVFLRTASIDGVAGNSN
                     AASDVAKMIFGGFGGRLVTVGILISVYGTINGYTLTGMRLPYVMAKENNLPFSKLFAK
                     LHDKTKVPVAAGILELVIAIGMMMIGGFDTLTDMLIFVIWIFYTMVFVGVILLRKKEP
                     DLFRPYKVPMYPFIPLVAIIGGTFIVSSTLITQTFLASMGIVLTLAGIPIYLYLKRR"
     gene            complement(32272..33225)
                     /locus_tag="ACG842_000893"
     CDS             complement(32272..33225)
                     /locus_tag="ACG842_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HFQ0247455.1"
                     /translation="MKKVLVTGCLGQIGSELITRLRQELGSENVIATDIRMPEVIEKN
                     DIFEILDVMDYEKMRSIAEQYQVDTLIHLAALLSAVAEAKPQLAWNLNVTGLVNALEV
                     AREFQLKFFTPSSIGAFGPNTPKDHTPQDTLQRPTTMYGVTKVAGELLCDYYYVKYGV
                     DTRGVRFPGLISYATLPGGGTTDYAVDIYYSAIKEGKYTSFIDQGTSMDMMYMPDAID
                     AIIKLMNADPDRLVHRNAFNISAMSFDPEEIKASIQKIIPDFEMDYDVDPVRQSIAES
                     WPNSLDVSCAQEEWGFSPKYDLDVMTKDMLEKLKEKLVETV"
     gene            complement(33327..34517)
                     /locus_tag="ACG842_000894"
     CDS             complement(33327..34517)
                     /locus_tag="ACG842_000894"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HFQ0247456.1"
                     /translation="MSSKELTMYLEKELDQLKEKGLYNTIDILESENGACIIVDGKKM
                     INLASNNYLGFANREELKKACIEATETYGVGAGAVRTINGSLKIHQQLEEKIAEFKGT
                     EAAIAFQSGFNCNMGAISAVMTKEDAILSDELNHASIIDGCRLSGAKIIRIKHQDMKD
                     LEKKAKEAIESKKYKKIMYITDGVFSMDGDIARLPEIIPIVEKYGLITYVDDAHGSGV
                     TGKGAGTVKHFGLSDKIDMQMGTLSKAIGVVGGYVAGSKTLIDWLKARSRPFLFSTSL
                     TPGAAAAALASITLMQEHPELVEKVWENANYFKEELKKVGYNIGMSETPITPVILGDE
                     KVTQTFSKKLIEHGIYAKPIVYPTVPLGTGRIRNMPTAEHTREMLDEAVAVYQKIGKE
                     MEII"
     gene            complement(34717..>34962)
                     /locus_tag="ACG842_000895"
     CDS             complement(34717..>34962)
                     /locus_tag="ACG842_000895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0247457.1"
                     /translation="IRKNRTVTRLLSQSFFRIQQVKLLRNIQRVKLKIADKRRWKFYV
                     FLLVYAINKLNIQNTIDFNERASINGCVVFICPGRVK"
BASE COUNT         9951 a         7642 c         6120 g        11249 t
ORIGIN      
        1 tacctaaaaa tttggatttc aacgatagaa acactgtttt ttatgtaatt agtccaagat
       61 ttttctagac ttttgcttta ctattcggcc aaattggttt ttcttctttt tttcgttata
      121 atgaatatac ttatgaaaag aaatgaggaa tgaaccatgt cctttgatgg tgtatttacc
      181 cacgcgatgg tcaacgaatt gcgtgaaacg ttactatctg gccggatctc gaaaatccat
      241 cagccttatg aaaatgaaat cgtgctggtg attcgttcac gcggcaaaaa ccatcgatta
      301 ttattatctg cgcacccaag ttatgctcgt gtacagatta cccaaatcga ttatcagaat
      361 cctgataatc cgcctaactt cgtgatgatg cttcgaaaat atttggatgg cgctatttta
      421 gaagacatcg aacaaatcga aaatgatcga gtgatccatt tccactttgc taaacgaaac
      481 gagttgggtg atctacaaaa tatcatattg atcgttgaat taatgggacg tcatagtacg
      541 atcgtcttag ttaatcgaga aactggaaaa atactggatg cgatcaaaca cattggaagc
      601 tctcaaaaca cttatcgttc tttgcttcct ggtgtagaat atgttgctcc gccaaaacaa
      661 gaggtcttga acccatttag ctccgagaaa gaaaaaatct ttcagcggct ttcccaaact
      721 gaactagatc caaaagcaat ccaacgtcag tttcagggaa tcggtttcga tatcgctcaa
      781 gagctgacca aaagattact ggaacgtcct aatgaaaaga tggttgtatg ggacgaattt
      841 ttctcagcca tcagccatca gcctattcct actttttacg aaacggaaaa taaagatttt
      901 ttcacaccga ttgcttatca ggtattgtcc gaacaggcat ctgctgtaac agcttatcca
      961 acactcagtc aattattaga tagctattat cacgaaaaag cagagaaaga tcgggcaaaa
     1021 caacaaggcg gagaactgat tcgaaaaatc gaaaatgaac taaaacgaaa taaaaataaa
     1081 ttgaaaaaac gtgaacaaac actcaaagaa tccgaaaacg ctgagaatta ccgtagaaat
     1141 ggcgaactac ttactacttt tctgacacaa gtaccacgcg gcgcaaaaga agttgtttta
     1201 cctaattatt atgaagaaga tcggccaatc aaaatagcac tagatccggc attgacacca
     1261 aatcaaaatg cacagaaata tttccatcga taccaaaagc tgaagaacgc tgttaagctg
     1321 attggcgaac aaatccaaga agccaaagat gaaatccaat atcttgaatc tgtcttatct
     1381 caactggaaa ttgccggccc aatggatatc gaagcaatta aagaagaatt gacttcagaa
     1441 ggatacctga aaaagaagaa cttaaaaaaa caaaaacgta agaaaccatc tcagcctgat
     1501 caatactttt cttctgacgg aacgctcatt cttgttggta aaaacaatct tcaaaatgac
     1561 cagctgacaa tgaagacagc taaaaaaacc gactattggc ttcatgcaaa aaatataccc
     1621 ggttcgcacg tcatcattaa aagtgacaag ccctcagatg aaacaattac agaagctgcc
     1681 gagttagctg cctatttttc aaaataccga tactctgccc aagtgccggt tgatcttgta
     1741 caggtaaagc atatccgaaa accaaatggc gccaagcctg gttatgttat ttatgaaaac
     1801 caaaagacga ttatcgtgac accagaagaa gaaaaaatta cagctatgaa acaaaacagg
     1861 taatcaatga gtgtttctag aatcatattt gcgtactttc ccacaacagc aattagttat
     1921 aaattgtttg ctacaaaaag aggagttgtg acaaaattct gtcacaaccc ctcttttttg
     1981 atcaacggtg ttcaataaac agcgttctca tctgcttttc ttagctctgc tggtccagta
     2041 accgagtaag ctctttgata aatctttgag cgatttcact gagcggacgt ttcgccgaat
     2101 aaatgatata aaattcattt agttcgcctt ctgccaaagg gattaaccgc aagtgctcgc
     2161 gaatccctcc aatcacaata ccagatcctg aagcgtaagc atcagtttca agaacgatat
     2221 tcatcaaagt acctcgatca ctcgtataaa tcactgtttc ttgatcaggg atctcgataa
     2281 ggtcttcata aaaatacggg aaattatttc catcctgtgt aaaacgaact tgaggataag
     2341 ataacaattc ttctttggtg atctctgcgt gttcagctaa tggatgtcct tgccttaaaa
     2401 aaatatgcgt tttgaatttt ccaatgctgc ggccacttag ctctccttgt tgtaaatatc
     2461 ttctgatacc agcttgatta tgctcattca tataaataat gccgatctca ctgtgctgct
     2521 ctttgacttc ttcgatcact ctcgctgttg tcgtctccat gatccgaaat tgtttgatct
     2581 cgtctttgaa ttcctcgagt agctgtgcgg tcaaaggacc taagaaatca taatgctggg
     2641 cagcgatcga aaaattgtct ctcttttcac tcgttagata gtgagtttct aatgtatcaa
     2701 gctggaccaa tattttctcg gcacgattta agaagtcttg cccttcttct gttaaatatg
     2761 cccctcgatt cgttcgagta aacagcgtga tccctaactc ggtttctaat tctttgatac
     2821 cagctgaaag acttggctgg gagacaaaca ttttctttgc cgcttctctg aaactaccat
     2881 tgtttgctac agcgaccacg tatttcatct gctgaatatt cattgctcaa actcctcgaa
     2941 ataataaatt tctcttccat tcaagggctg gattggtcgg ttgttcggtc gagcatactg
     3001 accatcgaag gggagaagaa actcttggtt cagttttcct acatgatcca ttacaaacca
     3061 ttggattggc ccgctggtag gcggagttgt caatgagcca atataatgga aatggctttt
     3121 ttgctccgga aagaacacat ccggatttac ccaatgctca tgtttttcta agttccaact
     3181 ttccccattt tcttgattat acagccaacc ttcctgtacc attagctggt acatgacacc
     3241 cacaactaga ttctcttttt tgctgttcat atgaacaaaa tgaaattcaa tatcttcctg
     3301 ttttccgttg atgatatgtt cagaaggcaa gtggaaatga atatctgtca aataatagcg
     3361 ttctttcttg aactctacat aactgagttg gtcaaaaggt actaaatgaa tcgtgttttt
     3421 gaattccttg tcagtaaacc gttgcttttg ataatgaaag gaaagcgtgt ctccttctat
     3481 tttggtttct tcccccttgg tcaaatgaat aggcgactgc aatggaaatt ccgcacctct
     3541 cgcaaaccaa tcacataaac tcgcccaatt ttccggacct ttgtctccag aatagcccca
     3601 ttcgacatcc atattctttt tcaactatac ttccccctgc atctcatatc atttttattt
     3661 ttcttctttc tttacaatcg ttgagacggt ttctaaaaac gcctcttttc cataaaattc
     3721 ctggttcaat agttccggaa atatctcttt aaagaaatac tgaacagaag gccgatgttc
     3781 aaagctttta atcgtagtca ctgcctcaat gaacatctcc ttataaactt tttccgggtt
     3841 atttttgatg atttcatcaa aagattcata aagaaggtca accttatctg aaatggctaa
     3901 tagttctcct tctacggttt catcttttcc ttcgaacaac cgacggcgat aaacttcttg
     3961 gaactcagct ggaatctctt ggttgatgaa ttcgtccgtc atcttttctt caactgtttg
     4021 cagcatccct cttaactctt tgcttgcgta tttcaccggc gttttgatat caccaataaa
     4081 acgctctgtg tagtcatgat tcaatgattt ttcatagagc agtttccaat cgatcggttg
     4141 tccattcgct tcttcaatgt ctcctagaac ttgcgcaata gatgctacac gatacgaatg
     4201 tgctgcaact gtatgctctg tatatttaaa aaatccaggt gcccttgtga tcgtctctaa
     4261 attgttcaat cctaaaataa attcattcaa ccccataaaa gttacctccc tttgagattt
     4321 ctttttccga atatcatatc attaaataac tgatattcgc aacatttccg ctctttacca
     4381 tttttgaaac agctccctta ccaaaaaata gagaaaatct atatataata gaaaaaagag
     4441 aagagattat tctcttcttt ctgctaacca gtcttagaaa aatgaatttt aaaaaaatca
     4501 aaaactgatc acgatagaag tttcttttca tacctgatga aaagatgctt ctcgcaatca
     4561 gtttttggat ttttgctatt ccaattgatg attctggaga cacaaatcgg atttctctat
     4621 cttcatcaag cgcgcttaaa tctgccaatt ttcaggatct tttttccatt cttttagcag
     4681 agttaattct tgctcatcga tataacgatc ttctaaagct gcttcaatca atacagaata
     4741 attggtcaac gtcataagag ggatttcggc tttttcgaaa tttgcttttc cttttggcaa
     4801 ctcatacgta aagatagccg caacaccaag gatatccgca ccttctcttt ttgctgcttc
     4861 agctgcttcc aagacacttc cgcctgtaga aatcaaatcc tcgatcacaa ccatcttctg
     4921 accttcaaat atacgtcctt cgatttggtt acctttccca tgatctttcg ccttacttcg
     4981 aatatagacc attggcaaat ccagtatttc cgctacccat gcggcatgag ggattcctgc
     5041 tgttgcagtt ccagcaatca cttgtacttc agggtaagct tctttgattt tgcttgctaa
     5101 tccttttgcg atctcttttc ggacttttgg atagctcatc gtgattcggt tatcacagta
     5161 aattgggctt ttgattccac ttgcccatgt aaaaggatta cttggattta aaaatactgc
     5221 ttcaatttct aataagtctt ttgcgatact ttgttcaaca ttcattttag gttagctcca
     5281 ttctgtttga atcgtttgat atgctttata aggattagtt gactgagtga ttggtcgacc
     5341 tataacgata aaagttgagc cgatcttttt tgcctgagaa ggtgtgacta ctcttgtctg
     5401 atctcctgcg actgttcctt ctggtcttat acccggagtt aagcaaacaa attcgtctcc
     5461 tgccatttct ttgatcgctt ctacttccca agcagaagaa accacaccat ctaatcctgc
     5521 ttttttggca cagctagcat aatggatcac acttttttct aaagaaactt cgattagttg
     5581 atcggcatgc atttcttctt cacttgtaga agtaagctga gtgatagcta gaagtagcgg
     5641 acgttttcca ccttctggag tcccctcttc taatcctcgc attgccgcct ccatcatacg
     5701 aataccgcca gcggcatgaa cacaggtgag atcaacacca agctttgcca atccccgcat
     5761 tgctttttcg accgtattcg gaatatcgtg aagtttcaga tctaaaaata cattatgacc
     5821 agcctctttt agataacgta cgatttcagg tccttcttgg tagaacaatt ccattccgat
     5881 tttcacaaac aatttttctt ctttcgggaa aaatcgaaga aagtcttcta cctcttgcct
     5941 agttgaaaaa tctaatgcaa taattggtcg ttggtctagt tgattcatcg ttcacgttcc
     6001 tctctcactt ctttgattaa tctttccagc gattcgatgc ctagttcgct cattcgcaca
     6061 gggagctctt taattagttt aggacagata tacggatcgg tgaaattcgc ggtccctata
     6121 gctacagcac ttgctccagc cataaacatc tcaagaacat catccactgt ctgcacgccg
     6181 cccatcccaa tgattggaag gctggatata cttgctactt ggtgaatcaa acgaatagcg
     6241 acaggcttga ttgctggtcc ggagagtcca cctgtttgat tggctaaaat cggttttctt
     6301 gtcttaaggt caatccgcat accaagaaga gtattgatca tcgtaaaccc atctgcccct
     6361 ccagcttcga ttgcctgagc gattggaacg atgtctgtta catttgggga caatttgaca
     6421 tagacaggaa cagaagaaac tttctttact gcttgtgtta attgaaaagc aacatcagaa
     6481 tctgttccaa aagcaattcc gccatgtttg acatttgggc aagaaatgtt cagctcgatt
     6541 gctttcacat ttggtgcttc gccaattttg ctacaaactt ctacatagtc ttcttcacaa
     6601 gcaccagcaa catttgcgat gatgggtaac tcattgtact cctctaattc tggaagaagt
     6661 ttatgcataa caacgtctag accaggattt tgcaaaccaa ttgcattcaa catgccagat
     6721 ggtgtttccg ccactcttgg cgtgggattg ccaaagcggg catttggagt cgttgccttg
     6781 accatgatcg atccaagctg attcaaatca tagtattttc cgtattcttt gccaaaaccg
     6841 aaacagccgc ttgccggcat gataggattt tttaattcta ggccaggaag tttgaccgct
     6901 aaaggattct cattcatata attacctctc ctattttaaa aatcggtcct tcgtcacaaa
     6961 ctttcacact ttttgttcct gtttcatctt ccggcgtatg acaaacgcaa gcatagcaag
     7021 cccctatccc acatgccatg cgggattcta atgataactg gacattttga tgcgaagaaa
     7081 acagctgttc cactgtcttc aataacccgt ttgaaccaca tgcaaaaaca gctgtaggtt
     7141 ccgtcttcgt tcctaacaat aaattaccga catttccgtg cataccgtat gttccatcgt
     7201 cagtagcgat tcttgtttct cctagacgat taaattcttt ttcataataa acgacttcat
     7261 gtgtggcaaa acctaaaaaa tgcgttggct taattccttt ttcgactaac tgttttgata
     7321 actcatatag aggagggaca ccgattcctc caccgattat aaatacttcg tctcctgtat
     7381 gaagattgga caaatcaaaa ccattgccta aaggacccat gacatctaaa tggtctccgg
     7441 cagaaagagt agagaagact ctagtcccct caccttccac ccgataaatg atacggcaag
     7501 tatttgtttc gtggtcgata tgattcaagc taatcggtcg gcgtaatagc aagtctgcac
     7561 gcggcacgag aatatgcaag aactgtcctg gttgctgcat ttcttttact agatcacctt
     7621 tcaatgtcat ttcaaaaatc tttggtgcca gctgtttttg ctgaacgatt tccatcattt
     7681 cttgtttcat ctgtcccctc ctgcaacggt ttttcgtctt tttgtcttaa catgtgctcg
     7741 ccgttaaata gggttagttg aaaaagcacg ggattccatc actcgaacga tcgcatctgc
     7801 tgtgtctaaa gaagtaaaca atggaatacc atgttcaact gcttctcttc ggatgatgaa
     7861 accgtcttta gaagcatttc cccgatcttt atcaatcgta ttgacaacca cttgtgtttt
     7921 accatctctt attaagtcga ctacattttt ttctgctgct tcctcagaaa ttttcgctac
     7981 tgtttcaacg accagaccat ttttttcgaa ataagcagct gtatgtttcg ttcctatcag
     8041 actatagcca atttctgtaa aacgtttagc taattctagt gcttcttctt ttgtttcgtc
     8101 cgcaatcgta aataatacgg ctccatattc aggcaagtgt aatccagaag cttcgaatgc
     8161 cttgtacaaa gctttgtcta attgtctgtc agatcccatt acttctccag tcgatttcat
     8221 ttcaggccct aagtaagtat caactttctg caatttcgta aaagaaaaga caggtgcttt
     8281 aacatgaact tcttttgttt ctggataaag tccgttttca taaccaagtg tctttagttt
     8341 ctctcccaaa atagctttcg tcgcaatttg tgccattgga attcctgtca ccttacttaa
     8401 gaatggaaca gtccgacttg ctcgaggatt gacttcgatc acataaacct cattttcatg
     8461 gatgacaaac tggatattca tcatcccgac acaattcaaa cctaatgcta agcggatcgt
     8521 gtaatcttcg atcgttttct tgatcgattc agataatgtt tgaggtggat acacagccat
     8581 tgaatcccct gaatggacac cagcacgctc aatatgctcc atgatccctg ggatgagtac
     8641 tgtttcaccg tcacaaatcg catccacttc gcattcacca ccaagcaagt aacggtccac
     8701 tagtactgga tgttctggag atgctttcac agcatgttcc atgtaatctt ctaaatctcg
     8761 ctggttttca acgatttcca tcgcacgtcc gcctaataca tagcttggac gaacaagaac
     8821 tggataacca attctcgaag cgataaccac ggcttcttct ttacttgtag cagtatcccc
     8881 taaaggttga ggaatatcca actctttcag cgcttgctcg aataaatcac gattttccgc
     8941 acgatccaag tcttcgatcg ttgtccctaa gatttttaca ccagcagcag tcaaaggttc
     9001 ggctaagttg attgctgtct gaccaccaaa ttgaacgatt actcctatcg gctgttctag
     9061 ctcgatcaca ttcatcacat cttcgaaggt caatggctca aaatataatt tatccgaaat
     9121 agaaaagtca gtcgaaactg tttcaggatt gctgttcatg atgattgctt cgtatcctgc
     9181 ctgctggatt gctttgacag aatgcacagt cgcatagtca aattcaaccc cttgtccgat
     9241 acggatagga ccagatccca gtactagaat tgagggcttt tctgatcgtg aactttcatt
     9301 ttcatattca taggaactat aaaaataagg tgtttttgat tcgaattccg ctgcacaagt
     9361 gtcaaccatc ttatatacag gaatcaaatc attttctttt ctcatctggc gaacggcttc
     9421 ttcgtttatt tcccacagct ccgccacttt tttgtctgta aaaccgtttt gtttcgctgt
     9481 tttcaatatc ttgtcatcca acggctgctg tttcaactgc tgttcgattt ctacgatatg
     9541 caacagttta tctaaaaaga ataaatcgat ttttgtcaaa tccgctaatt cttctatggt
     9601 gtaccctctt ctaatagctt cagctacata aaacaggcga tcatcttgtg ctcgaaccac
     9661 tttttggatt aggtcatcgt ccgtgatgtc tgccaactca ggaagttcga tatggtgtgt
     9721 accgatttct aaagaacgca cagctttcaa taaagactct tcgatattcc gaccgatagc
     9781 catcacttca ccagttgctt tcatctgcgt gcctaaaagc cgttctccgt tttcaaactt
     9841 atcaaacggc cagcgaggaa ttttagctac aacataatct aatgcaggtt caaattctgc
     9901 aaaagtcgtt cctgtaacag gatttttcat ttcgtctaat gttaatccaa gcgctatttt
     9961 agcagctagc ttagcgatcg gatagccagt cgccttactt gctaacgcag atgaacgaga
    10021 aacacgcgga ttgacttcaa tcacataata attaaaacta tgtggatcaa gagcgagttg
    10081 gacattacag cctccctcga tcttaagtgc tcgaataatc gaaagagaag catcacgaag
    10141 catttgatat tcatggtcag acaatgtttg actaggggca aatacgatag aatcacctgt
    10201 atggataccc accgggtcaa aattttccat attgcagaca acgattgcat tatcagccga
    10261 atctctcatc acttcatatt caatttcttt aaaacctgca atacttcgtt caatcaaaca
    10321 ttgggtagcg ggggaaagac ttaatccatt ttctgatacg atacgtaatt cttcttcatt
    10381 ttcacacata cctccaccag ttccgcctaa agtaaaggct ggacgaacga tgacaggata
    10441 gccgatttta tttgcaaaat ctacagcttc ttctactgta ttgacgattt cagactcggg
    10501 gattggctga ttcaattctt tcatcaactg tttgaataga tcacgatctt ctgcttgatc
    10561 gatcgcagaa agctttgttc ctagaagttc tatgttcaat tcttctaaaa tacctgatgc
    10621 tgccagctcc attgccatat tgaggcctgt ctgtccgcct aaagttggaa ggagagcatc
    10681 tggacgctct tttcgaagaa tccttgatac aaattcaagt gtgatcggtt cgatatatac
    10741 ttgatctgcc acttctttat ccgtcatgat cgtcgcagga ttagaattta ctaaaaccac
    10801 ttcatatcct tcttctttta aggcaaggca ggcttgtgtt cctgcgtagt caaattctgc
    10861 ggcttgacca atgatgatcg gaccagatcc aatcaccata attttcttga tatctgtacg
    10921 tttcggcatc tgtttactgc tcctttcctg cgtccatcat ttccataaat tcatcaaata
    10981 aatgtagtgc atcatgcgga ccaggggcag catctggatg aaactgcaca ctgaaagcgg
    11041 gataatctct gtgtttgact ccttctactg ttccgtcatt tacttctaca tgagttacga
    11101 tgagtttttc tggatcaatc gtggcttcat cgactgcata gccatgattt tgagaagtaa
    11161 aatcaattct tccggtcgct acttctcgta ccgggtgatt taatcctcga tgtccgaatt
    11221 tcattttata ggtatctgca ccgttggcta aagcaaataa ctggtgaccg agacaaatac
    11281 caaaaatcgg tacttttcct tggatttttt tgatcatttc gattgcttct ggcacatctt
    11341 ttggatcacc aggtccattc gtcagcatga ctccgtctgg tgatagcgct aaaatcgtct
    11401 cggcatctgt gttataggga agtactgtga ggttacagtt tcttttactc aattctcgta
    11461 aaatactatg tttcaagccg aagtcaatga caatgacttt ccgacctgtt cctggacttg
    11521 gataaggttt gttagttgag acttgctgca cttgattggt tgctagaacg gttgctttca
    11581 gctgatcaaa tgcatgttcg aaggaatctc cagcatcaac tatactagct ttcatcgttc
    11641 cgacttcacg gattttgcga gtaagggcgc gcgtatcgat acctgatatt ccaggaattc
    11701 ctttttgttt caaaaatgaa tctagcgtca tttggtttcg ccagtttgaa gctacacgtg
    11761 catgttcttt tactactact cctttgcatg ttggagaaat cgattcataa tcatctctat
    11821 ttaccccata attacccacc ataggatagg taaatgtaat gatttgaccg ttaaaacttt
    11881 gatcagtgat agtttcttga taaccagtca tacttgttgt aaaaacgatt tctccgaaga
    11941 cactagcatc tgcaccaaaa gcctttcctt cgaaaactgt tccatcttct aatataagca
    12001 aacgttccat acttttattc tcccttctgc cacacaagtt ttccgttgac aaaagtcatt
    12061 tgtgtttcgc cttttacttt ccagccgata aaaggtgtat tctccccttt tgataaaaag
    12121 tcttctttat cgatcgtgca agtttgtgta atatcaaaaa ctgctacatc agcgggggca
    12181 ccaaccgtta gcgttcctgc attcaaacca aaaatctcag ccggtttaac tgccaaccaa
    12241 ttgatcactt gttccaacgt gaatcgacca gtttctacaa aatgagtata gatcagttga
    12301 aacgctgttt cgcttcctac gataccaaat ggtgatttca tgaaactttg gcttttttct
    12361 tctaatccat ggggggcatg gtctgtagcg atgcaatcga tcgtaccgtc aagcaagcct
    12421 tcgatcaaag cttctctgtc ctcccgtcct cttagcggcg ggttcatttt ccaaaagccg
    12481 aaatcttcgg gaatatcttc gtcgatcaaa ataagatgat ggggtgaaac ttctgctgtt
    12541 acgtggattc ctgctttttt tgcgtctctg attacacgga cactttcttt agtagaaaca
    12601 tggcaaacat ggtaatgaac accagttgct tcagctagca acagatctct agcaatttga
    12661 gaagactcgg ttacgcttaa gatcccggga agaccaagtt cttctgcctt cttgccagcg
    12721 tgcataacgc cgccaaataa taaagattca tcttctgtat gggctactag ggctttattg
    12781 ttttttgcag cttctttcat agccagatac atcgtcccag ctgtttggac acctacaccg
    12841 tcatttgtga aagcaaaagc accttcttca atcagtgctt cttgatcaac taatttctca
    12901 cttcgcagat tttctgtgat tggcgcatac tgcaatacct tcacaactgc atcttttcta
    12961 atgatgtcat atacttgtct cagcttttct gccgtatcag gtacaggatt taaattcggc
    13021 atcgcgcata cagtagtgaa accgccacga gcagctgagc gagtcccagc ttcgatcgtc
    13081 tccttgtatg taaatcctgg ctctctcaaa tgaacatgga cgtccactaa accaggtgtg
    13141 atcaattgtc cctttgcatc aaacacttca tcaaattcgg cttctgagaa tcctgtaccg
    13201 attgctttga ttcgcccatc ttctatccaa atttctgctg gtgtcgtcat attttttcgt
    13261 gtattgatct gaccgttttt aatgagtgtt ttcatttttt catcctccta taattacgct
    13321 tttccattta gcactgcttc taagattgcc attctgacga atactccatt gctcatttgc
    13381 tgaacgatcc gcgactgtaa tccttccact aatgaatcag ctaattctac atcacggtta
    13441 actggtgctg ggtgcataat gattgcatgt ttttgcaact tcttcgcacg ctcaactgtt
    13501 aacccatgtt cctgatggta gccttctttt gagaaacttt cttttccatc atggcgttcg
    13561 tgttgcaccc gaagcatcat catgacatca acttgatcta gtagttcatc caatggcata
    13621 taatgtccgt acacttcgaa ttcatcgtcg taccattctc taggacctga gaagaatact
    13681 tgtgcgccta gacgttttaa catctgcata ttggatttag ctacgcgaga gtgagtgata
    13741 tcacctacga tggcaacttt cagaccttca aaatgaccaa attcttcata aatggtcatc
    13801 aaatcaagca agcattgggt tggatgctgt ccgcttccgt ctcctccatt gataatcgaa
    13861 cactggattg ttttactttg tatcagttcg tcataatagt tctcatcacc atgacggata
    13921 accgctacgt ctacgcctaa tgctgacatc gtcagtactg tatcgtatag tgtttcccct
    13981 ttttggacag agcttgtgct tgcttcaaat tcaatcactt ctagccctag ttttttctcc
    14041 gctacatcga agcttttatg tgtgcgagta ctattttcga aaaagaggtt tgtagcaaag
    14101 tactggcgct tttgcggagt ccaattagca ccttgcttga attcctgccc tctgcggatc
    14161 aagcccataa cttcttgatc agtaagtgcc tcaacggtca ataaatgctt taagctgata
    14221 cgttctgatt tgatgatcat aatgtttcct ccttagtttt cttcgcttcg tgaagtttct
    14281 ggtaagataa gatttaaaat gatgccaagt acagttgcta atgccattgc cgacaatgta
    14341 aatgtgccta cttcaaagac gagtccgcca attcctacta ctaagataac tgaagcaatc
    14401 aacagatttt tctttttatc aaaatcgatt ttgttctcga ttaaaatctt cattccactt
    14461 gcggcaatta ctccaaataa gataaaactg attcctgaga tgactggtcc agggatactt
    14521 aaaatcaaag cgcttaattt tcctacgaat cctaaaatga ctgcaaatac tgcagctcca
    14581 ccgatcacaa acacgctatg gacacgtgtg atagccagaa caccaatgtt ttcaccgtaa
    14641 cttgttacag gaggacctcc gacaaaggct gccacgattt gtgctaatcc atcacctgtc
    14701 aacgtcttat gaagacccgg ttcttcaaag aaattacgtt tagttagttt attcaatacc
    14761 ataagatggc cgatatgctc tgtcatcgtc acaaaagcga tgggtgccat tgttgtgatt
    14821 gctccaagat atagctttgg ttcatattgt acaaagggaa tctcaaagtt aggaacagca
    14881 aaccaggcag cgtctacgat tggcttagta tccacgattc caaacagcaa ggctacgatg
    14941 tatccgctaa taatacctag taaaatcgga atcaatccga gaaaacctct tagccacatg
    15001 ttgaagaaaa ttgttaatcc taatgtgatc agcgctacag caatatactt gaaatcatac
    15061 gtgccctgat tatacatagc atttgttgct gcatttgccg caagtcccaa cccgataacc
    15121 atgatgactg gaccgacgac gattggaggc aagattttat ccagccatgc tgaaccgatc
    15181 tttttgatga tcagcgcaac aatcaaatag actaatccag ttgtgactgc tccttgtgca
    15241 atcgctggat aaccatctgt tttcatcaaa agctgcatcg ctgcaatgaa agcgaagctg
    15301 cttcctaagt acgcaggtat ctttccttta gtagtaaata agtacaccaa tgtacctagt
    15361 cctgaactga ctagcgcaat acctggatcg atacctacta agatggggac taacacggtt
    15421 gctccgaaca tcgtgaacaa gtgttgtaaa cttaatccta cccaatgtcc tggggttggg
    15481 cgatcgtgga tatctaacac cacgtcatca ttatgaaatc cttttttctc cgacatgttt
    15541 ctctctcctc taatcttcct ctttcaaaat catgatgcga tcttgtccat caagctcttg
    15601 catctctact aaaatctctt ccgcccgaga tgttggaatg ttcttcccaa cataatctgc
    15661 acggattggc aattctcgat gcccacgatc gaccaggact gctaatgaaa tttttcttgg
    15721 tctgccaaaa tccattactg cgtccattgc tgctcgaatc gttcgtcctg tatacaaaac
    15781 atcatcaatc aaaataactt cttttccttc taaggaaact gggatgtcag aagaatgaag
    15841 ttccggttct tctggatttt cttttttgtc atctcggtat aaagtgatat ccagttctcc
    15901 tacaggaata tcgatatctt ctagctgctt caggcgttcg gcaattcttg atgcaatgta
    15961 gattcctcgt gttttgatac ctaccaatac gatatcttgg atactatggt ttctctcgat
    16021 aatctcataa gtaattcgag tcaatgctcg cttcatggtt acctgatcta ctacttcttt
    16081 tgcttgcatt ttcattcccc ctactttttt gcataaaaaa actcctgccc taagaggaca
    16141 ggaggaatgt ttagactgag cgtatactga tacactgata gctatactat gtcaatgagc
    16201 aatctactta tggcgcacca tacgtatcct tgctcccacc cttcttagcc tcacgggact
    16261 aaacttaaag gaattctatt aagttttatg ctttcgttgt taattactat accgttatct
    16321 aaattaaaat gcaatgtttt ttcttaattt ttctaaagtt ttttcaaaaa cttctggaag
    16381 aggtgcttca aagatcatct tttcgtttgt agtcgggtgc gtaaaaccta acatttgcgc
    16441 atggagaaac tgaccattgc ctttcaaagt tttcttcggt ccgtatactg gatctccagc
    16501 taacggataa ccgatatact tcatatgcac acggatctgg tgtgttcttc cggtttctag
    16561 ttttaattca accagagtaa atccttcgaa acgttccaat acagtaaaat gtgtgacagc
    16621 cgacttccca tcttctctta tcgcctgcat tttccggttt tccttcgaac ggccgattgg
    16681 cgcattgatg gttccttttt cgtgaggaat ctctccatgg actaaagcaa catatttgcg
    16741 caaagaggtc ttatctttta attgctgagc taatgattcg tgtgcctggt catttttggc
    16801 aaccattagc agtccggaag tatctttgtc gattcggtga acaatacctg gacgaataac
    16861 gccgttgatc gtagaaagat ccttgatatg gtacatcagc gcattgacca ttgtgccatg
    16921 cgtatgacct gctgaaggat gaacgaccat tccttgaggt ttgtttatca ccgctacatc
    16981 ttcatcttcg taaacgatcg tcaacgggat attttctggg accacatcta atgtttctgg
    17041 ttcggggatc aagacagtaa tctcgtctcc ttgttttact ttgtaatttg ctttgactgg
    17101 ctgtccattg acagaaaccg ctccattttt cagccacagc tgaatttgcg aacgactgta
    17161 atcggtgaat aaattagtca atactttatc gatcctacca gattcatggt gaatcgtcgt
    17221 tttaagttct gtcatttttt tctttgatcc ttttcatcta aaataatata aattaacata
    17281 caaatcactc cacatacaag tgtcacatct gctacgttaa aaatcggaaa gctcataaaa
    17341 tctgtctgga acatatctac tacatagcca agacgaatac ggtcgataaa attgccaatc
    17401 gcaccagcta gaatcaaact caatccaatc atgaaccagc gattaccttt ttcacgattt
    17461 ttaatcatca atgtgagaat cacaacgact gcaatgagtg tcactatagt aaagaaccac
    17521 atcttccctt ctagcatact ccatgcggct ccagtgtttc gaatatgatt cagtgacaaa
    17581 atacctggta taagattttt cgtctctcca agcgctagat tatcagtgat ccaccatttg
    17641 accaactgat ccactcctac tagcaaagcg ctgaatatcc aataaataat taacaaaaaa
    17701 tcaatccttt tctatttgtt tataaatatc acgaggtcgt acgatcccga gcagattacc
    17761 atcgggattc ccatctttag tgataagtac ggcttccaaa gtcccttgct cacgaaacat
    17821 cgcttcaacc tcgtaaatat gtgttttagc tggaacaaaa tgataatttg tgtactttcc
    17881 gttttggatg atcaaatcca acgctcgttt atttgttaaa tcaaccggac ctttttggct
    17941 ttcttttgct agccaaaacc cgatattacg taaagtaatc aatgctacaa attttccttt
    18001 attgtataac ggaaattgtg aatacctttt ttctgctact atctttaaca gttcaagtaa
    18061 cggaagatct tgttcaaaac ctgttacacg ttttgcaaat cgaggcagca cggtttcagg
    18121 ctgaatcagt tcttgttcga tcagctggat tcgcttgact gtccaatcat ttggctctgc
    18181 aatgacaaaa tcgactgcga tccgatcatg gacgatcgca ttccttaact gagcaagctg
    18241 taaaagatca tcttcgtatt ttctgatcat taattgtttt ctttgagcta ggcgtctgac
    18301 aagttcggta aatcccatat ttcgtgcatt tcccatctcg tctctcatcc atttttcgat
    18361 tcgattgaaa ctgattaaaa aaagatcaga gtttgccata attttcctcc ttgatcaaat
    18421 atgcccggtt ttttatatag aaaaagaacg caatcaacac aataactgca aaaaaactga
    18481 aaaataaatt ttgtaggaaa acggccccca acccagttgc tagaaataaa aacggataga
    18541 tttgaagaat caagtaattc attgtgatca cataagcagg ctttgcttct ttcactgtat
    18601 aaacggggct ttttagccaa taaaaggtga cctgcatcgt aaagggcact tccagatcaa
    18661 gcgttatctt ttgttcatga ctgagaacac tgcgtttttc attgttgatt cttagctgaa
    18721 tcgagctacc cataccgtaa aaaccggttt ttcttataaa tgtcgttttc atcataccct
    18781 cctattatgc tttagtatag ctcaaaatac aataaagaaa aagaatgatc ctagtgtaaa
    18841 caaaattaat tccctatcgt ctaaaaaaag aagctgtagt caaaatacag ctccttcgat
    18901 ggtagattat aatcaagctt aaaacaatcc caacacttct ccgtcatttg tcacgtccat
    18961 attcaatgca gcaggttttt taggcaaacc aggcatcgtc ataacatctc cagtcaaggc
    19021 tactaaaaat cctgccccta attttgggat gatttcgcgg atcgtgatgg tgaaatcttc
    19081 cggtgcacct agcagtgaag gatcatcaga aaatgaatac tgtgtttttg ccatacaaat
    19141 cggcagtttg tcccatccat tcttcgtaaa ctcttctatt tgtttttgcg cttttttgct
    19201 gaaaacaaca ccatttcctc cgtaaatttt cttgacgatt gtttctgtct tttcttgtat
    19261 cgtttcttct aggcggtata atggcttgta attagcttct tttctatcaa tcaatcgtac
    19321 tacagctttt gctaaatcaa cgccgccttc tgctccatct gcccatacac tcgctctctt
    19381 ggcaagaatc ccctgatttt cacacaagtg ctccaacagt gtgagttcat tgtctgtatc
    19441 tgttacgaat tcattgattg cgacgatcac aggtagctga tattgttcca tattacgtat
    19501 atggcgcttt aaatttgcaa aacctacttt caaagcatca agattctcat ttttaagttc
    19561 atcttttttc attccgccat gcattttcaa tgcacggaca gtggcgacaa tcaccacagc
    19621 atccggcgct tttttcaagt taggtacttt gatatctaaa aatttctctc cgccaagatc
    19681 tgcaccaaat cctgcctctg tgacaacgta atctgctaat ctcaatgctg tcttggtagc
    19741 taaaatacta ttgcagccat gagcgatatt ggcaaacggt cccccgtgga caaaagctgg
    19801 tgttccgtaa attgtttgta caagattcgg cttgattgca tctttcagca gtaatgccag
    19861 agcgccttct gctttcaagt ctccagcagt cacaggctga cggtcaaatg tatagccgac
    19921 aacgatcctt gcaagacgag cctttaaatc ttcaaggtct gccgcaagac acaagatagc
    19981 catgatttcg ctggcaactg tgatatcaaa cccatcttct cttggaacac cttggattgg
    20041 cccacccaag ccaacaatta ctttacgcag ttcacgatca ttgagatcga ctacccgttt
    20101 ccaaatcact cggcgggcat cgatgttcaa ttcatttcct tgatgaatat gattatctaa
    20161 aagagcagat aatgcattat tagctgttgt gatagcatgc atgtcaccag taaaatgcaa
    20221 gttgatatct tccataggca atacctgtgc atatcctccg cctgcagcac cacctttgat
    20281 tcccatcaca ggaccaagtg agggttctcg caatgcaata acagattttt tattgatttt
    20341 attgagcgcg tctcctaacc ctacagtgat cgttgacttt ccttcgccag ctggtgttgg
    20401 attgatggaa gtaactaaga tcaaatgccc ctcagcgtta tcctttgatc gattgattcc
    20461 agaaaaattg attttcgcct tatatttccc atacagttcc aaatcgtctt ccttaagatc
    20521 taatttacta gccacttcaa caatcggttc tagctctgtt tcttgtgaaa tttgcagatc
    20581 attcttcatt tgattacccc ttttcacgaa ctttattcaa caaaatatct tctttgttaa
    20641 gatatgccaa ttataccttt ttttattcag aaaaaaaaca taaaattaca ggtttttaat
    20701 ttatttgacg tacttataaa tactctttat aatgaaggct atgatcagac tttagaaagg
    20761 tcgtgagtgc tttgaccaca ggaattgtaa aatggtttga caacaaaaaa ggatacggct
    20821 ttatcagtta tgatgacaca gaagaaattt ttgtacattt tactgcgatt gaagaagagg
    20881 gattcaaaac tttggaagaa aatcaagtgg tcgaatttga gatcatcgaa gggaatcgag
    20941 gaacacaagc tgcccatgtc aagaaaggca acgttcagac taccgatact agtatgcatg
    21001 aatagataaa caaatagagt gagacgctaa acgggatgaa ccgtacgtct cactcttttc
    21061 ttttcctgta aaaattcgtt tttttaggtt ctgattttta aaattagctt tttctttttt
    21121 tcgtataagg ataaggaatt gattctgatt tgggtccgtt tttttcaaaa tgatcaaaaa
    21181 ggactacttg atcttttttt cgcatcatga aaacagatgg ggctttctca tcttgcaaca
    21241 tcatttgcac cccagtatta gttggcgtca aagatcgaat ctgtccgaca gatatttctc
    21301 ggtctttcca aaaagcagca taacgtataa tcacagagtt tgattttaaa ataaaagaac
    21361 gccgcaaacc tagaaataga aaaacaagaa aaatacctaa aacaaaattg cttttccaat
    21421 aaggacctgt gttttctaaa gaaagaatca gactaaaaaa cagaatgatc aaggtcaatg
    21481 accaatagat gatcgtatgt gccagttcag gctgccatcg aatatttttt ttcattctct
    21541 aacctctcac cgattcagta atgatataat cttaccataa cattagtttg aacaaaaagg
    21601 agaatctctt tttatgttaa aagtatacat tgatgcctct acaaaaggca accctggtcc
    21661 tagtggaggt ggaatcttga ttgtctatca aggtaaacaa gaacagctat ctatcccgct
    21721 ttctataggg acaaaccatc aagcagagtt cgaagtcttt ttaaaaactt tagaatttct
    21781 aaagaaaaac gacttacaga atgaaacgat tttctgtttt tcagacagca aaacactagt
    21841 ttctacggtc gacaaaaacg caacgaagaa cgagctcttc cttccctacc ttcaaagaat
    21901 ccaagaactt ctatccgaat tttctttatt gatcctgcaa tggttgcccg aaaacaaaaa
    21961 taaaggagca gataatcttg cacgtcaggc tctccataaa caattaaaag aaaaaaagac
    22021 tctttaaatt aaaaacttta aggagtcttt ttatagtttc tcatttaata taaaagataa
    22081 ttgtgatcaa acgtaatttc caattggtaa tctccgattt tttcttctat ttgactacgt
    22141 aagattcttt ctagttctgt atcaacaaac tggaatttct cagggaccac tacatcaaat
    22201 tgcagcgttt ttccttgatg aattactcta aaatcatgaa tattaagccc tgtacctgtt
    22261 tcaagaagaa tttgctttaa ctgccgcaag atattatagt atttttcgtt atcaacaggc
    22321 attggatcca agtgacagac aagttccaca tccaaagact ttttaaagtc tttttctatc
    22381 agatcaataa tcttatgagc cacattcaaa ttcattgatt catctacttc gatgtggact
    22441 gatgcaaagc gtttattggg tccatagtta tgcaccaata aatcatgata gccaagtata
    22501 tcatcgcaag catttaaatg ctcttccatt tcttcaatct ctgctggcgt tgggcgactt
    22561 cctaatagtt cgtatacaaa atttcgaagc atcgtcaatc cactataaag gatatataat
    22621 gctaaagcaa aacctatata tccatcaata cgccatccag ttacccattc gattgctgct
    22681 gaaagcaata cagccagtgt agtaaagaca tcattataac tatctgttgc tgtagctctc
    22741 aatgtttcag aatcaatgga ttgcgcaata acctgataca ttttgccttg tgcaaatttc
    22801 aaaaagatgg aaatgattag gacaaaaaag acgatgggtg ttaaagagac attttctgga
    22861 tggatgattt tttcagcaga agactctaaa aattgaaatc ctacataagt aatgatgatc
    22921 gaaacaaata acccactgat atattcgaag cgttcgtgtc cataaggatg ttcctgatct
    22981 gctggctttg cagcaatttt aaatccgatc aaagtcaata tggatgaagc agtatctgat
    23041 agactgttca ctgcatcagc cataatggag acacttcctg aaaaaagccc gatcattagc
    23101 ttagagacaa acaagatcag attcgtaacc aatcctaaga tacccgctag tactcctttt
    23161 tttgtccgct gttgcggtat ttgtttcttt ttttcttctt gtttcttagc caagtttttt
    23221 accccttcta gtttgcttgt tgctcaggta aaaagatgga gaggacgatt gttttcctct
    23281 cctctcttcc tttttctatc tatcgtgcgt cctattacta ttaaaagatg gtcattattc
    23341 tggtttcaac ggtgttgctg caaataacgc ttttgctagt aatggtgtta tgattcccgc
    23401 acctattgct tctggtatcc cattgattgc tgcaacccct gctaaggttt taaataaaag
    23461 agcagagtct acaccatagg catctgccgt tgcacctgta taaaataatc ccatgaatcc
    23521 taacactaag actgtattga ccaatgaacc cagtacccct gcaaaggctg agctgatcgt
    23581 tattgattta tttttttgat aaatccagtg gaaaaccaag cctgctacta agccaacaag
    23641 tactctggga actaccgaga taatcggatt cgtgaacact aatgtatcaa atggcgttgt
    23701 tggcatggca taggcacgaa caattgtagc gattccccaa aacaagccaa tcaccatgcc
    23761 atcttttggg cctaatacta ctgcagcaat aattactgtg atatggataa ttgttaaact
    23821 aatgaatcct aatggaataa accccagcca aggaaccatc gcttgtacga tgatgattgc
    23881 taacaagatc gcacgtaaga ctagtcgaaa ggtcttattt ctactattca ttttttcacc
    23941 cctttttatt tatactaatc gtaaaaattt ctgtcacttt cgtcaactaa agaccatccg
    24001 aatccgacca tttgattaga tgcgccatcc aattgaaaaa agtatgaggg caagacttgt
    24061 ttgtcttcgc ccccataatc atgtttcgtt caatgtttga acttttgttt ttctgtcaaa
    24121 cgtaaacgcc ctattgttgc gcaacccatt ctttcgcagc tttaagtgca tctggaatac
    24181 cagcaggatt tttcccgcca gcttgtgcca tgtctggacg tccaccaccg ccaccgccta
    24241 ctttcggtgc gattgcttta atcaagtcgc cagcttttag tccagcttca tttgattctt
    24301 tcgtcattgc cgctagcaga ctgacttttc cttcttgttc gtttgctaaa acaagaatat
    24361 ctgaaatatt tttttgtttc cattggtcag ctaattgacg cagctgattc atatctttca
    24421 cttttacttg tgctgcaatg attgtttttc cattgatcgt ttctggatct ttgaatacat
    24481 caccagcttg ttgattcgct aacttgctag ctaattgttc attttccttt tgtagctctc
    24541 ttacttgctg ttgcaattgt tccgcttttg aaacaacttc tttcaattga ggtgatttaa
    24601 caattgttgc tacttgtttt agttgttttt cttcttcttc aagtagttcg tacgcttctt
    24661 tacttgttac agcctcgatt cgtcgcacgc ctgcaccaat tcctgattca gaaacgattt
    24721 tgaagattcc gatgttttct gtatttgcta catgtgtgcc tccgcacagt tcgattgacc
    24781 agccgccaat atttactact cgaacgtcat gtccgtattt ttcaccaaac aaagccattg
    24841 cacccatgtt tttggctgta tcgatatctg tttcaattgt ttcaactggg atagcttccc
    24901 agattttttc gttgacgatt ctttccattt cagctaattc atctgaagtg atttgaccaa
    24961 agtgggtaaa gtcgaatcgt aaatgtccag gagcgactaa agaaccagct tgatttgcat
    25021 gctcgccaag tacttctttc aatgccttgt gcagtagatg ggttgctgta tggtttttga
    25081 tgatccgatt gcgcatcgat tgatcgatca atagatgata ggtttgtcct tcactcattg
    25141 gtgcttttac ttctacggtg tgcataaatt gaccattagg cgctttttga acatctgtaa
    25201 caactgctac gatttcgcct acttcattca tgatccatcc ttgatccgct acttgcccac
    25261 ccatttctgc atagaaaggt gtttgatcaa agatcatttg agcttcacct tctgcgactt
    25321 cattcaaaag agactcacct ttaacgatga ccaataattg actatctgat tcttgttctg
    25381 tgtatccaat gaatttactt tctactttga tatctgtcaa taaagcagat tgtaccccca
    25441 ttgatttttc agtactgcgg gcagagcgag cacgttcgcg ttgtgcatcc atttcttttt
    25501 caaaaccagc atgatcaact ttcagtcctt catcttctgc cacctcttcc gttaattcta
    25561 ccgggaagcc atatgtgtcg tatagtttga agatgtcttt tcctgctaga gtcgtcccgt
    25621 tttcgttttt tacttcttgg atcacttgat ttaagatttc caaaccttca ttgattgttt
    25681 cattgaaacg ttcttcttct gtacggataa ctttttcaat aaattctttt tgttgaagta
    25741 cttcaggata ataacttacc atgatttccc caacaacagg gactaaacga tataagaatg
    25801 cttgttcaat acctaatttt ttcccatgca tgacagcacg acgaagcaaa cggcgtaaga
    25861 cataccctct cccttcattc gatggcaatg caccgtcgcc aatagcaaat gaaagggcgc
    25921 gaatatgatc tgcgattact ttgtaagaaa tatctgttac tgggttttca ccatatttta
    25981 cttgtttgct tagcttttct acggcgtgga taataggcat aaacaagtct gtctcaaagt
    26041 tcgttgacgc atcttgaacg attgagacca tgcgttccaa tcccatcccc gtatcaatgt
    26101 ttttatgagg aagtggttca aatgtgttat caggcttatg gttgaattct gaaaatacta
    26161 agttccagat ttctaaataa cgttcatttt ctcctcctgg atagttttca ggatcgtctt
    26221 ctgctaaatc gttataagct tctccacggt catagaaaat ttccgtatcc ggaccacatg
    26281 ggcctgcacc gatatcccag aagttgtctt ctacatcaat aatgtgatct agtggcaggc
    26341 caacttcttc atgccagatg cgttttgctt ctgtatcttt tggataaact gttacataaa
    26401 gtttttctgg atcgaatgcc atccattttg gatcagtcaa aaattcccat gcccagtgga
    26461 tggcttcatt tttgaaatag tcaccaatcg aaaaattacc cagcatctca aacatcgtat
    26521 ggtgccgtgc tgttttaccg acattttcga tatcatttgt tcgaatagat ttttgggcat
    26581 ttgtgattct tggattttct ggcacaactg aaccatcaaa atattttttc aatgttgcaa
    26641 caccagagtt gatccacaat aaggttggat cgttcacggg gatcaaagac gcactaggtt
    26701 caatcgtatg tcctttagat tggaagaaat caagaaacat ttgtcgaact tgtgcactac
    26761 ttaattgttt catgtcatta cctgctttct ataaattaaa tacaaaaaaa caccatcaca
    26821 gccaaggacg aaaactcgcg gtaccacctt gattgcaatg atgtgtaatc attacctctt
    26881 aaactcgata acgctgagct gcgtcggtat ttctaccaaa atagaaaaag ggagcaattt
    26941 gattagtgac tattcttttt tcagccaaga aagaattttc tgtgagtctt aaaaaacaaa
    27001 tcatatcctt ttcgtactta attagtatac gcattctatt gatcactgtc aatatctcaa
    27061 tttttatttt catttccttt tggtcatact ccacgatttt tggtacgata agaataaagg
    27121 aatcaataca tctctgctat tcatgacaag gaggaatatg atgaaaaaaa tagttggagc
    27181 ggatgaatgg atacgcgcag ctagctgctt tttgagatat caagtttttg tattagaaca
    27241 agggattcct gcagaactag aattcgacgg aaaagatatc ccgcaacaaa cctattatct
    27301 atttcttgat caaaaaagac cagtagctac tttgcgttat gaaaagaaaa cacctacttg
    27361 cttcaagccg gaacgatttt gcgtagcaaa agaataccga ggaaaagggt taggcggtca
    27421 gttgcttgaa gctgctgaaa aacgggcggt ttcagaaggt tgtcagacta gttatgtagt
    27481 agctgaaatg acggctgtag atttttatga aaaacaaggg tatcatactt gcacagaacc
    27541 ttttattgaa gatgaagtac tttgtatcgg aatgaaaaaa agtctagctt aaaaacaaga
    27601 gattgtgaca cgccttttgt cacaacctct tgttttttat ttatgtgaat ttttcttatt
    27661 tttacgagca gtacggcttt gctgtctgcg ttcgattttg cgttcttttt tatttttttc
    27721 tgcaattgcc caatcgattt ttttcttata accaggcttg atctttttct ttttcttctt
    27781 cacaagacca atcagcgtgg gatctaatac atcttttgat ttttctcgtt tggcacgtct
    27841 attacgatcg tatgttttga cgatttcccc gtttttgatt tcttttggtt caaatgagac
    27901 gcccaatttt tctatcgcat caatggcttg ctcatcattt ggcgaatata acgtaatggc
    27961 cgttcctttt aagccattac gtccagtacg tccgacacga tggataaaga agtctagatc
    28021 ttctggaatt tctgcattaa taacatgaga gacaccttcg atatcgatcc ctctagcagc
    28081 taaatcagtc gctacaacat attgatactc cagattctgt acttgtcgca tcactcgttt
    28141 tctctctctt ggggtgatgt ctccatgaat ctttgctact ttcaagcctt ggtttttcaa
    28201 gtaatcggca atttcatcca catgctgttt tgtattggca aatacgattg ctaaataagg
    28261 atgaccaatt gtcagcagtt gataaatcat ttgattctca tctttacctt ttgttgaaat
    28321 cagccaattt tcaatgtctt cagaaataac tgcttttggt ttgatttctt caatgattgg
    28381 attttccatg tattttcgta agaatggacg aagtttttct ggaatagtgg cagaaaaaac
    28441 taaaaactgc agcttttctg gtaatcgact tgcaatttgg tctacttcat ttaaaaaacc
    28501 catatccaat gtcatatccg cttcatcgac aacaaatgca aatgcggtgt gaatcttcag
    28561 cgcttgttca ttcatcatgt ctaatattct accaggcgtg cctactacaa cctgtggctg
    28621 ttggtgatgc agacgattta attgacgctg tttgtctgta ccgccgacaa aattcgagac
    28681 acggatctcc ggtgtagaaa atttagcgat ttgtgtagct gcttcataga tctgtgcagc
    28741 tagttcccta cttggagctg taataacaac ttgtacttcc tctttttctg cattgatttt
    28801 ttcaaacaaa ggcaacaaaa acgtatgtgt ttttcctgta ccagtttgag attgcccaat
    28861 gatatttttt ccttttgcaa ttaaaggaat caaacgttcc tggacctctg tgggctgttt
    28921 gaagcccttt gcggttaacg cttcattgat aaacggtttg aagcggaata tatcaaaatt
    28981 attcattcgt gcacctcttc tctttaaatt atcacgactt tggcattata acacagacaa
    29041 agcgtttttg ctcagctaat ttcgagaatc atcatccttt gtttggccaa tcgtgctaac
    29101 agtatatgtg tgactagctt tttcataaaa cagaatccgt ttttttctca aaaatgatag
    29161 caagatattt aaaataaaaa taccttgtag taacaagaca ccgagagaaa tgaatactgc
    29221 actcatctgc aacaccgtat tcgttgtcga attcatgatt tgctgaagca actgccccaa
    29281 ccagcttaaa ccataaaaaa gaaagtacag atacccgtag cggatcatca atgcttttaa
    29341 agaaattcgt tcgcggtctt cttccaccaa acggatacgc acaatttttt tgccaagtgt
    29401 ccatccattg gttataaacg gtacaatcat aaagtatacc cctacttcta ataaaagcca
    29461 ccagatatga gaagtaactg cccctgaatc gaacgcaatt ttaaagaaaa tcgccgctac
    29521 caaattaata agagataaaa atgtccagtc gatcaaataa gcgaccaagc gtcgagtcaa
    29581 tgagacagtc tttccttttt cataagaaag ttcatccatt cgtcttcttg taggaagcat
    29641 aaatgtaaat aacggcgtaa cgacaaaacc aatgattcca cccaatgtat ttgtcactaa
    29701 atcattcaca tcaaataagc ggtatggtct tgggtagata aaataaagtc cacttagctg
    29761 cgtcaattca aaaaatagag aaaggcagaa actagcaatc atcgttttca taaaagaaag
    29821 ccggaaataa tatcttaaat aaatgccaaa cggtatgagt aataaaacat taaaagctgg
    29881 ttccaaaaat acactctgct tcattgccgg caggtaggta ctaggttcag acaagaccaa
    29941 taccgtgttg tctctgaaat gttgccatga agcaaataaa tgcagctcca tctttggccc
    30001 tgtcatttgg gcaaccgtct cctttgccgg aagtggtaag atcaccaaaa aatacgcaca
    30061 caataaataa aaaacgaaag aatacaaaat aactgccctt gttactagaa atgttccata
    30121 ctttcgatat tcataaataa aaaaaacaga tgagatcgct aaggctaaaa atggaaaaac
    30181 aatcagcgca aaacgaatcg gttcgatata acttgccata tgactcctcc ttgattcttt
    30241 gtatctctct tatcttagca gtcctttgct gtttcaagca agtaacgagg ctattatttc
    30301 tcatttcttt caaagtaaag taagataaac acaaaggggg tgtcgagatg ctgaatatgt
    30361 actttgtttt tggcgtacct atttttttac tgttccttta tgctacgatc gcttatgtaa
    30421 gaaaaagaac tacaattcat tatttaggat ttattctcct gattatttcg ggtttcatgc
    30481 tagtcttcaa tcttcagact tggcagcaag cacttcttga aatggataaa atgactccac
    30541 atgctttgtc taaagtgctt ggctaccctg tctatctgat ttggcttccc attttcattt
    30601 ctggttgctt ggttttatta aatatttatc gcggcgtacg ccgaatagtt cagttacgca
    30661 aatcaaagta aaagacagaa atcggttgag cccgatttct gtcttttact ttgctatttc
    30721 atcgtagaca aaacgacatt tacgtgatca aaattaacga gtacttttga catttcttcc
    30781 acgtactcct catttaaata aaatattttg tttggcagag aagtgcagtt ctaataaaat
    30841 tcgaatgttt tagctatcta cgttttaaat agagataaat cgggatacca gcaagtgtca
    30901 atactatccc catcgaagct aaaaaggtct gagtgataag cgtgcttgat acgataaaag
    30961 tccctccaat gattgctact agtggaataa acggatacat tggaactttg tatggtcgaa
    31021 acaaatcagg ttctttttta cgaagaagaa tgacaccaac aaagaccatc gtataaaaaa
    31081 tccaaatgac aaagatcagc atatccgtta gtgtatcaaa accaccgatc atcatcatgc
    31141 caatagcgat gactagctct aagatacctg cggctacagg aactttagtt ttatcatgaa
    31201 gtttagcaaa gagcttgcta aaaggcaggt tgttttcttt tgccataaca taagggagac
    31261 gcattccagt aagggtatag ccattgatgg ttccataaac agaaatcaag ataccaactg
    31321 tgaccagtct tccaccgaag ccgccaaaaa tcatttttgc aacgtcactt gccgcattgc
    31381 tgtttcctgc gactccgtcg atagatgcgg ttcttaagaa caccgcattt actaataaat
    31441 aaacgatcat gattccgatg attcctaaag aaattgcttt tggcaaatct ttcgccggct
    31501 ttttcaattc tcccgaaata tttcctacgt gtatccaacc gtcgtatgcg aacatcgttg
    31561 ctagcaagcc cgctcccaag gctgagaaaa atgaaagatt ttctcctgct tggatcggaa
    31621 acagttggaa gtctactcct tcttgtcgga acaaaccgaa aatgacgatc acaaacaaag
    31681 gaataagctt gcagacaagc gtaatcgact gaaaagcccc tccagcttta gaacctaaaa
    31741 aattgatcaa caggatcgat acagcagctg taacagctac gggaacaatc attgactggg
    31801 aaagaccaaa tagattgacg aactgtgtac cgaaaatgat cgacaaggct gctacatttg
    31861 ctggaaaata aatcacgact tgcgcccagc ccaataaaaa agctgctgta tttccgtaaa
    31921 tccgttcgat atatttaatc atccctcctg tttctggaat agcagctgcc agttcagctc
    31981 ctgttagtcc tgcacagaca gaaatcatac caccgagaaa ccaagaaaac atatgcaaac
    32041 tggcggatcc tgtaacttct gctacgcttg cggctttaaa aaatacccct gcaccgatca
    32101 ctgtgcccat aacagtcgat aaagcagtaa aaaatcccat cgtacgtttt aattcatttt
    32161 ctatttttac ttcttttact ggcgtggctt gatattcttc catcccttgt cccctttcat
    32221 accatagcca atcccaaaag tataaaaaac tttttggatg tggttcttgt tttataccgt
    32281 ctctaccaat ttttctttca gtttttccaa catatccttt gtcatcacat ctaagtcgta
    32341 tttcggcgaa aagccccatt cctcttgcgc acaagaaaca tctagtgaat ttggccaaga
    32401 ttctgctatt gactgacgta caggatccac atcataatcc atttcaaaat ctggtatgat
    32461 tttttgaata gatgctttga tctcttccgg gtcaaaagac atcgccgata tattgaaggc
    32521 atttcgatga accaatcgat caggatctgc attcatgagt tttataatgg catcgatcgc
    32581 atctggcata tacatcatat ccattgatgt accttgatca atgaaactag tgtactttcc
    32641 ttctttgatt gctgaataat aaatatcgac tgcgtagtct gtcgtaccgc ctcctggcaa
    32701 ggttgcataa gaaatcaatc cagggaaacg gacaccacga gtatctacgc catatttcac
    32761 ataataataa tcacatagta gttctccagc taccttcgtt accccataca tggttgtagg
    32821 acgctgaaga gtatcttgtg gggtatgatc ttttggagta tttggaccaa acgcgccgat
    32881 tgaacttggc gtaaaaaatt tcagctggaa ttctctagcg acttccaatg cattgaccag
    32941 cccagtcacg ttcaaattcc aggctaattg tggttttgct tctgcaacag ctgacaaaag
    33001 agcagccaaa tgaatcaatg tgtccacttg atattgttct gctatgctgc gcattttttc
    33061 gtaatccatt acatctagga tctcaaagat atcgtttttc tcaatgactt caggcatgcg
    33121 tatatctgtc gcaatgacat tttcactgcc tagttcttga cgtagccgtg tgatcaactc
    33181 tgaaccaatt tgtcctaaac atcctgtcac caatactttt ttcatgatga tcctccctga
    33241 tcttttttac tactatcttc agatgattaa caatatatgc gtgaacatgt tctctaattt
    33301 taaatagacg aaaaaactaa atttagttaa ataatttcca tttcttttcc tattttttga
    33361 taaactgcta ctgcttcatc aagcatttcc ctcgtatgtt ccgcagtcgg catatttctg
    33421 atcctgcctg tcccaagagg cacagttgga taaacgattg gtttagcata aatcccatgt
    33481 tcgatcagct tcttagagaa agtctgagtc actttttcgt ctcccaaaat gactggagta
    33541 ataggtgttt cagacatacc gatgttatag cctacttttt tcaattcttc tttaaaataa
    33601 ttagcatttt cccaaacttt ttcgacaagt tccggatgtt cttgcatcaa agtaatcgaa
    33661 gccaatgcag ctgcagctgc acctggagtt agcgaagtag aaaataggaa tggccgactt
    33721 ctagctttca accagtcgat tagtgttttc gatccagcta cataaccccc aacaacacct
    33781 atagctttcg ataacgtgcc catttgcata tcgattttgt cgcttaatcc aaaatgcttg
    33841 acggttcctg caccttttcc agttacgcct gagccgtgcg catcatcgac gtaggtgatc
    33901 agtccatatt tttcaacaat cggaataatt tccggcaatc gtgcaatgtc tccatccatg
    33961 gagaaaacac catctgtgat atacatgatt tttttatatt tcttactttc tatcgcttcc
    34021 tttgcttttt tctcaaggtc tttcatatct tgatgtttaa ttcggatgat ttttgctccg
    34081 gataagcggc aaccgtcaat aatagatgca tggtttaatt catccgataa gatagcatct
    34141 tcttttgtca ttactgcaga gatagcgccc atattgcagt tgaatccaga ttgaaaggcg
    34201 attgctgctt ctgtcccttt gaattctgcg attttttctt ctaattgctg atggattttc
    34261 agcgaaccat tgatcgttcg gactgctcct gctcctacac cataagtttc ggttgcttca
    34321 atacatgctt ttttcaattc ttcgcgattg gcaaatccaa gataattgtt ggatgctaga
    34381 ttgatcatct tttttccgtc tacgatgata caagcaccat tttcactttc tagtatgtcg
    34441 atcgtattgt ataacccctt ttctttcagt tgatccaatt ctttctctaa atacattgtt
    34501 agttctttgg aagacattgt ctccacctcc atattcatct gtcttgcaga acataccaaa
    34561 catatatcgt gtcattcatc tacaaagcaa gtatgctata tttttgataa cgcttacact
    34621 gattatgaga attatttaac cacatttttt atttacagta tctttttact ttcaaatgac
    34681 ttatatagta tgaacaatta ccttttagat aatgatctat tttactctac ctgggcagat
    34741 aaatacaaca caaccattga tagaagccct ttcgttaaaa tcaattgtat tctgaatatt
    34801 taatttattt attgcataga ccaataaaaa tacataaaat ttccatcttc gtttatcagc
    34861 aatttttaat tttactctct gaatatttct taaaagtttt acttgttgaa ttcgaaaaaa
    34921 ggactgtgac aaaagtcttg tcacagtcct attcttccga at
//
LOCUS       DAZKLM010000020        34578 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3764
            SAMEA115706913-rid22582563.denovo.020, whole genome shotgun
            sequence.
ACCESSION   DAZKLM010000020 DAZKLM010000000
VERSION     DAZKLM010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706913
            Sequence Read Archive: ERR13196647
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34578)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34578)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287823.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287823.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,004
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,849
            CDSs (with protein)               :: 2,849
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 41 of 96
            Pseudo Genes (incomplete)         :: 52 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 15 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34578
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706913-rid22582563.denovo.020"
                     /isolate="VRE3764"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(248..2140)
                     /locus_tag="ACG842_000896"
     CDS             complement(248..2140)
                     /locus_tag="ACG842_000896"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0247458.1"
                     /translation="MSKTNTRPSHGPAGGPGHGGPGRNLGVKGEKPKNFWGTVRRLFG
                     YMSKRMVAIIAVFVLAIAAVIFQIQTPKVLGKATTEIFKGVMAGAQQMQQGQQVTKFP
                     IDFDKIAQIIATVIVMYLISAVFNFLQQFIMTRVSQRTVYELRHDLEAKMNRVPISYY
                     DTHTNGDIMSRAINDMDNIASTLQQNLTQFVTSAVTLIGVIVMMLSISWQLTLVALLM
                     IPLSLIIVMIVAPKSQVFFADQQKSLGLLNNQVEETYGGHTIVKTFNHTEKDQEVFEK
                     ENQNLYAAGWKAQFISAIIMPLMNFVKNLGYVFVAVLGGVKVANGNMTLGDVQAFLQY
                     TTQFSQPITQLASLMNTIQSTVASAERVFEVLDEEEMSNEKSGLPEEKDTPYKVRFEN
                     VQFGYTPDNLLMTDFNLDVKPGEMVAIVGPTGAGKTTLINLLERFYDVSGGSIRYDGV
                     DTRDLSRDELRAQFSMVLQDTWLFTGSIYDNIKYGNDDATDEQIIEAAKAAHVDDFVR
                     KLPEGYNTVLNEDASNISQGQRQLITIARAFLANPDVLILDEATSSVDTRTEILIQKA
                     MNRLLENRTSFVVAHRLSTIRDADNIIVMNHGSIVETGNHDTLMEKDGFYADLYNSQF
                     SEEEAS"
     gene            complement(2137..3864)
                     /locus_tag="ACG842_000897"
     CDS             complement(2137..3864)
                     /locus_tag="ACG842_000897"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0247459.1"
                     /translation="MLKIVKRISLTSAIAAAVFMVIQVLADLYLPTLTSNIIDKGVAQ
                     GDVDYIWHTGFVMIGFSLISILAAIANTFFATRESQKLGKQLRTDVYKKSESLTKDAF
                     DKYGTASLITRTTNDVTQIQMVTQMFLRMMINAPITLIGASILAYQKDHQLTKIFLVV
                     IPVMIILIGGIMYFAVPLFKSMQKKTDRLNLVFREGLTGVRVIRAFDKTRFEENRFDL
                     ANKDYTNTAIKVNTIVALMMPMMTLIMSGTNVAITWFGGHYIADMTLEVGNLIAFMTY
                     AMQILISFMMLSAIFIMVPRAQASADRINEVLDEKIGIHDPENPKTVSFAGKNATLAF
                     NHVNYRYHGAEKLALEDIDFQAKSGEIVAIIGGTGSGKTTLVNLIPRLYDIESGSIQI
                     NGTDISDMTQYNLRELMGFVPQKAVLFSGTIRDNMQYGKPDATDEMIWKALEIAQAKD
                     FVSEMEDGLDSHVEQGGGNFSGGQRQRLAIARALVKTADIYVFDDSFSALDFKTDANL
                     RQALKTNMKESITVLVAQRVSTVMDADMILVLDEGKLVGKGTHEELLATNETYQEIVH
                     SQLREEDLA"
     gene            complement(3880..4530)
                     /locus_tag="ACG842_000898"
     CDS             complement(3880..4530)
                     /locus_tag="ACG842_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HFQ0247460.1"
                     /translation="MPTQTFFHLPKEKQKRLIEAARIEFSRVPLKEASIANIVKLADI
                     PRGSFYQYFEDKEDLYFYYFETMRRDSSKDMLQLMKESDGDLFKGFESYFSKMIYEIL
                     EGENASFYRNLFMNMDYRASRKVAPHTSRQKTPHDPHKEHKQDAKELLELIDRTKLKV
                     KNDHELELLIQLVMNTVFTTIAHSYRLFSTSEDYSVEQAISEFNLKLSWLKNGVYK"
     gene            4734..5030
                     /locus_tag="ACG842_000899"
     CDS             4734..5030
                     /locus_tag="ACG842_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFQ0247461.1"
                     /translation="MAQSISVQDFNKLPLDADSIVLDTRNTLDYTKNHLSHSLSIPAA
                     ILPRQLKNLDPKKLYYILSYTGRRSEALADQLTANGFRAVYIIGGMEALQSSVA"
     gene            5202..5822
                     /locus_tag="ACG842_000900"
     CDS             5202..5822
                     /locus_tag="ACG842_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HFQ0247462.1"
                     /translation="MASVLVVKGHPLTAEESRTVKALTSFLTSYKENHPDDEVTVLEL
                     YRDDIPEIDEELLSGWEALRAGAEFTSLSESQQQKIARFNELTDQFLAADKIVIANAL
                     WNLNIPTKLKAWFDTVNVAGKTFRYTENGPEGLVTGKKALHIQSNGGVYNGQDFASQY
                     VKGILNFVGIDQVDQLFIEGIDYDPDRADELMQNALDKAAALGKSF"
     gene            complement(5914..7080)
                     /locus_tag="ACG842_000901"
     CDS             complement(5914..7080)
                     /locus_tag="ACG842_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFQ0247463.1"
                     /translation="MEGKNKKNRPVSWFWRWFLNNQVVTALLVVLLILLIVFLFTKVS
                     YLFAPIWQFLAIVGLPIILAGILYYLMNPVVDYFEKRKVPRLYSIIGLFVLVVALIAW
                     GSVVIIPKIQEQTVSFISNFPQYVDTIDNKLTEILRDPLFNQFREQLETAGDKFMNSA
                     GDMIQDISKSTVQSLGSFVGAVATILVALLTMPFILFYLLKDGKQLAPYFVKFLPTRM
                     QKPTLNVLSEMNSQVSSYIRGQLTVAFAVAIMFMIGFAVIGLEYAVTLGIIAGFLNLI
                     PYLGSFLAMIPAIFLAIVAGPFMIIKVLIVFAVEQTIEGRLISPLVLGNQLAIHPVTI
                     LLVLLTSGKLFGIIGVILGIPVYAAAKVVITHVFEWYTTISSLYEENPLKKQKE"
     gene            complement(7092..7445)
                     /locus_tag="ACG842_000902"
     CDS             complement(7092..7445)
                     /locus_tag="ACG842_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HFQ0247464.1"
                     /translation="MIQAKITEIGTEAINKEEPMLILFDKTATAALRKYSVIQEVSKD
                     EPFSLKAGGTIAFDDQVYTIDYVGPMANGNLETVAHVSLIFDACPEEGQIANGIYLHP
                     HRLPAIGLGSQIFYN"
     gene            7573..8595
                     /locus_tag="ACG842_000903"
     CDS             7573..8595
                     /locus_tag="ACG842_000903"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HFQ0247465.1"
                     /translation="MIEKIILGTYTRRESKGIYTIDLDTEKEELANLSLAVEENSPTY
                     VAKSKAGNLYTVTSVDGLGGAGAYDKDFHFLNAVTESGAPLCYVAVDEARQLLYGANY
                     HKGEVNVYHILGDGELKAADSIFHQEATGPHKNQDHAHVHYTDLTPDQRLVVCDLGTD
                     RVYTYDVSENGKLNLVTTFTAEPGTGPRHLVFHPNGQFAYLFGELDSTVRVLSYNKET
                     GAFEELQKISTLPEEFDGENGGAAIRISNDGKFLYASNRGHNSIAVFAVFEDGSHIQN
                     KQIISTEGDFPRDFALDPSNNFVVCANQNTDNLTLYRRHPETGLLELLKKDVYVPECV
                     CVYFDR"
     gene            8614..8775
                     /locus_tag="ACG842_000904"
     CDS             8614..8775
                     /locus_tag="ACG842_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0247466.1"
                     /translation="MNQQDVLDRLREELNIPFFDGKIEDKEYSEEEYQKLKADLQNYF
                     EQYVQNVEN"
     gene            complement(9034..9468)
                     /locus_tag="ACG842_000905"
     CDS             complement(9034..9468)
                     /locus_tag="ACG842_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0247467.1"
                     /translation="MLPNIGYYVQSIDQIVKETEAIGEGMHPKYEVIRNAIDEGKTSE
                     LDKSTLEEIILLFEEGTKKYRAMLKKVSTLRPPAKAMGIHKNFEKAYLSYVAGCEEMI
                     QSIDLESGVDEDLFNTSEAKQDQATDQLTAAIQKMTNLLLKK"
     gene            complement(9578..11437)
                     /locus_tag="ACG842_000906"
     CDS             complement(9578..11437)
                     /locus_tag="ACG842_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HFQ0247468.1"
                     /translation="MKRYISRLSTKQKKILKGTAAIISIFFLVVFSNLFLQWCQNDLS
                     VDLALKFAFSWHTEKFFLACLVLLIILIFLIALAGSVPLGSLTYVVAIGVLGFANYMK
                     MSYRQEPIYPDDLKMITEIGLLKDMTGTMLFTVILAAAGTVIGLFCWYMFRSLKKGRR
                     FQLIRLTTLLVAIGLLGYISNFNNPDNLLRKAYNKTALWIPYSQKMNYYNTGFIGGFL
                     YNLKVEPMDEPEGYSKEKIKEITEKYQKLADEKNKTVEEESPNIVFVMSESFSDPSRL
                     NGVEVSGEPLADYYEVADQTYSGNMLSQNYGGGTANIEFEALTGFSMALFNAQLTTPY
                     TMLVPKMDQLPSIVSALNAQSYQTTAIHPYNTSMYKREDVYQTLGFDQFISERTMTYT
                     DTIENNPYISDESAYKEVMTLLKEEKTPQFIHLVTMQTHMPYDGKYDNLDYPAKTSDG
                     SGTLDLENYLQDISYSSTALKQFTEELKNLSRRTLVVFWGDHLPGIYSDTIQAKNDKQ
                     TLHETQFLMFDSKGELEKQTTHDAITSPFYFAANLMEQTNQTTNGFYQLLLSLEQELP
                     AFERELYYQNGQWYKEAQFNRSQQEIYDEYQLIQYDIVAGKQYSLADGFFEHE"
     gene            11438..11578
                     /locus_tag="ACG842_000907"
     CDS             11438..11578
                     /locus_tag="ACG842_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoglycerate dehydrogenase"
                     /protein_id="HFQ0247469.1"
                     /translation="MLAPSFLSIKECCFSYFSCTKFESKERKEELMKSKYSLSLWPFW
                     FL"
     gene            complement(11555..12715)
                     /locus_tag="ACG842_000908"
     CDS             complement(11555..12715)
                     /locus_tag="ACG842_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HFQ0247470.1"
                     /translation="MYHVHLIDAIPEQEIEKLQGEYFKIIDDLPAEGIVIRSTKVKDE
                     WLTPDLLAISRAGVGVNTINVEKASENGTIVMNTPGVNANAVKELVLCCLLLSSRPII
                     EASRMVQTLTGPNILEQAENKRSAYVGRELQGKTIGLLGLGAIGTKVALSCYSLGMDV
                     LGYSIRDAQLDYVRQADLETVLSTSDYIVVMLPLTEDTKGLIDQANIEKMKKDAVLIN
                     VGRSEIVDKYAVMQALEKQHLAKYLTDFPEEEFLENDRILMLPHLGGSTQEALADSGR
                     LAVEALKDYLLFGTVREAVNYPSARMFFQAPFRFTIFYQKKTNILAEIFSLLNENELA
                     IADISRNHKNEHVYILIDIDSTDFEQLNQVKQQLEKISGVRKVRLLKKPERP"
     gene            complement(13022..14446)
                     /gene="araA"
                     /locus_tag="ACG842_000909"
     CDS             complement(13022..14446)
                     /gene="araA"
                     /locus_tag="ACG842_000909"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008733 - L-arabinose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0005996 - monosaccharide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HFQ0247471.1"
                     /translation="MLNIGEKEFWFVVGSQHLYGEEALREVKKHAQEMVDELNENGQL
                     PYPIRLQELAVTADTITKIMKEVNYREEVAGVITWMHTFSPAKMWIRGTKLLQKPLLH
                     LATQYNESIPWKTIDMDFMNLNQSAHGDREYGFINARLNKQNKIVVGYWKRPEIQKEI
                     ADWMDVAVAYNESFGIKVARFGDNMRNVGVTEGDKVEAQIQFGWTVDYFGIGDLVQVI
                     DRVSDEEVDQLFEEYKELYTFDYGDYEEKTWEEHVKVQAQQEIGIRRFLEEGGYNAFT
                     TNFEDLYGMKQLPGLAVQRLMAEGYGFAGEGDWKTAAIDRLLKIMARGKDTGFMEDYT
                     YELASGQEAILESHMMEVDPTLAATKPRIVVSPLSMGDREDPARLVFDGKAGEGVVVS
                     MADFGTHYKLLINEVEAFEPTTEAPNLPVARVLWKTKPNFHEGVHSWIQAGGGHHTVV
                     SLNLTTDQIETWAKLVELETVVIR"
     gene            complement(14462..15160)
                     /locus_tag="ACG842_000910"
     CDS             complement(14462..15160)
                     /locus_tag="ACG842_000910"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008742 - L-ribulose-phosphate 4-epimerase
                     activity [Evidence IEA];
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019569 - L-arabinose catabolic process to
                     xylulose 5-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HFQ0247472.1"
                     /translation="MLEQLKEEVYQANLDLPKHGLVKYTWGNVSAFDPETRYFVIKPS
                     GVSYEEMTADDMVVVDLDNHIIEGKLNPSSDTPTHAVLYRSFPQIGGIVHTHSTWATI
                     WAQAGLDVPAMGTTHADTFYGSVPCARFLTQQEIDSGYEYETGKVIIETFKERKIDPL
                     AIPGVLLHGHGPFTWGKDAQSAVMNAVVLDEVCKMNLFTRQLNSFSEELPQRILDKHY
                     LRKHGKNAYYGQNK"
     gene            complement(15117..16778)
                     /locus_tag="ACG842_000911"
     CDS             complement(15117..16778)
                     /locus_tag="ACG842_000911"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004856 - D-xylulokinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0005998 - xylulose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HFQ0247473.1"
                     /translation="MKEVNAQSMLDVIDGRTSLGIEFGSTRIKAVLIDSSFTPIAAGS
                     YEWENKLENGIWTYSLEDIWKGLRVSYRKMAAEVFEIYGENLTTIGSIGFSAMMHGYL
                     AFDSKEQLLVPFRTWRNTITEEAERILTEKFQYNIPQRWSIAHLYQSILNKEEHVKEI
                     NFLTTLAGYVHWQLTGEKVLGVGDASGMFPIDPSTKTYHQEMKNIFGQLIEKAHVAID
                     LDHILPKVLVAGEEAGKLSESGAYLIDPTGNLRPGIPVCPPEGDAGTGMVATNSVAKR
                     TGNISAGTSAFAMIVLEKELENVHPEIDMVTTPSGELVGMVHTNNCSSDINAWVSIFE
                     EFTNSLGMEISRDKLFTVLFNKALEGDTDCGGLLSYGYFSGENITGVNEGRPLFVRTP
                     GSRFDLANFMRIHLASAFGAMRIGMDILKSENVQIDRLVGHGGIFKTPEVGQRILASA
                     MEAPVTVMDTAGEGGAWGIALLAAYMMDRHGKSLENFLAEDVFGQDQGITITPSAEEI
                     AGYQIFMRRYREGIDIEKTAIHQLQEKGVIENAGTTERRSIPSKS"
     gene            16996..18396
                     /locus_tag="ACG842_000912"
     CDS             16996..18396
                     /locus_tag="ACG842_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051119 - sugar transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HFQ0247474.1"
                     /translation="MKKEKKVSSSFIYFFGAFGGILFGYDIGVMTGALPFLQHDWGLA
                     GKASLIGWITSSVMLGAILGGSLSGLLSDKLGRRKMILLSALIFMAGSVLSASAPHNG
                     SYFLIAARILLGLAVGAASALVPAYMSEMAPARLRGRLSGINQVMIASGMLLSYVADY
                     LLKVLPETMAWRVMLGLAAVPALILFFGVLALPESPRFLMQSGRLEEAKRVLNYIRTP
                     KEAEQEFEQIQLNVKQEKTTGTSWHTLFLEKYRSLVIAGIGVAVFQQFQGANAIFYYI
                     PLIVEKATGHAASDALMWPIIQGIILVAGSLLFLVIADKFNRRTLLKIGGSVMGLSFI
                     LPAVLGTVLDAHTNSLLILLFLCIYVAFYSCTWAPLTWVIIGEIFPLAVRGRASGLAS
                     SFNWIGSFLVGLLFPVMTASMSQEIVFGIFGIICFLGVLFIQEIVPETRGKSLEEIEQ
                     SASKKTYPKRISIHHS"
     gene            complement(18648..19727)
                     /locus_tag="ACG842_000913"
     CDS             complement(18648..19727)
                     /locus_tag="ACG842_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFQ0247475.1"
                     /translation="MAKYQDIAEEIKQKILREEYEVNTTIPSEFKLQEIYDVSRHTIR
                     QAIAVLVNEGYLRKEKGSGTYVDDSYKKEKTPLNRTKTIGVITTYLSDYIFPSIIRGI
                     ENELRESGYSLLLASTNNDHELEKECLKKMIAYGVDGLIVEPTKSNQYNPNLALYVAL
                     REQGIPMVMINAAYEELNASYICLDDVKVGFMAAEYLIKKGHQRLLFITKIDDLQGKY
                     RMKGFISACEQYNVRFLTEDIITFTTETRETIAEKTVQRLKEEQALDGIICYNDQIAS
                     LMLDKLTENGYHIPDDLSIVGTDDSSLSHLGNKKLTTLTHPKEKLGQDAARWIVTTIE
                     KGQAQPNILYEPVLVERETVKENEK"
     gene            complement(19813..20772)
                     /locus_tag="ACG842_000914"
     CDS             complement(19813..20772)
                     /locus_tag="ACG842_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HFQ0247476.1"
                     /translation="MMKTLYNNPIVRQRADPWVYKHTDGYYYFTGSVPGYQVIELRRA
                     KSLNELEHAASLIVWQAHEEGPMSELIWAPEVHYINGKWYIYFAASNHKTIRDESHHH
                     RMFVLENQHIDPMNTDWIEKGQIKTKRETFSLDGTTFSSQGKLYYVWAQQDPEITGNS
                     NLYLSEMDNPWTLKGKQILLSIPEYEWEKRGFSVNEGPAVLTRNGKIFITYSGSATDE
                     NYAMGLLWADEHKNLLLKESWHKLSEPVFVSSEKNKQFGPGHNSFTVSEDGCFDILIY
                     HARPEKNQKGDPLDNPNRHANAQCFTWNEQGFPEFGEPVPYAL"
     gene            complement(20784..22295)
                     /locus_tag="ACG842_000915"
     CDS             complement(20784..22295)
                     /locus_tag="ACG842_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046556 - alpha-L-arabinofuranosidase
                     activity [Evidence IEA];
                     GO_process: GO:0046373 - L-arabinose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-arabinofuranosidase C-terminal
                     domain-containing protein"
                     /protein_id="HFQ0247477.1"
                     /translation="MKVKIREDFTVGEISSRLFGSFVEHMGSVVYTGIYEPGHETADG
                     DGFRQDVLSYVKELELGVIRYPGGNFTSGYDWVDTVGRKEQRPKKIDLAWRGIEPNSF
                     GLHEFFKWISHVQAKPIFTVNLGTKGIDEAKNLVEYCNFPKGTFWSDLRIKNGQKKPF
                     GVKLWCLGNELDGPWQVGAKSAYEYGRLANEASKVMKLVDDSIETILVGSSTPRLPSY
                     PEWDRIALEEAYENVDYLALHNYIDKYDEEDLTKPPKNERDDTATYLAKAYRFDRQIE
                     EIVATCDYVKGYLRSEKTMYLAFDEWNVHAQPEKRHRDFEVGSPIDWCYFTMEDTLLF
                     GSLGLAILRHADRVKISCQSLLVNTIPLILTDAKGQAWRNPTYYVMQHLSKYAKGQVL
                     KQQIICPVYDCEKIKNVPYVDSVIVDNGNQLTIFLINRTNEQQKITIEHDFNLLDQGE
                     HYTLTSSDLLDKNTREEPEKIKPEINYFSIEKHDELNVGIEKYSWNVIRIKKM"
     gene            complement(22317..22880)
                     /locus_tag="ACG842_000916"
     CDS             complement(22317..22880)
                     /locus_tag="ACG842_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HFQ0247478.1"
                     /translation="MKIVETKFYGQLIVVSDYILLGILWVIVCLPVITIVPATCAVFY
                     VMAQWKGEGSGNIIYFFFQGIKQHFGKNLFLSLLTIIIYFLVNQLLEEQTIVLSISGY
                     VVLLIYSMFLLSWIYVISENKDITFLQIFEQSAWKVFFDFLRNILCCVLLLGFVLLIF
                     LFPPFIFIFAGGVWKLVSIILTIGNER"
     gene            complement(22886..23749)
                     /locus_tag="ACG842_000917"
     CDS             complement(22886..23749)
                     /locus_tag="ACG842_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0247479.1"
                     /translation="MAPESSITVTVNSRKAVSGKKIIRTISMLVILLVIAFPFLWLII
                     SSFKHEKDIISFPPRIFADSYTLDNYIKVWTTIPLLDYIKNTVIFAGGTVITSVFFDS
                     LAGYAFARMRFKGKSVLFYFVLLTMMIPFQVFMIPLFIEVNLLGMLDTYAGLIIPRMT
                     TAFGIFMMRSFFITLPDSLEEAARIDGLSEFNIFLKIMLPLSKPTLLSLGIFTLMNSW
                     NDLLYPLILTSSSKMRTLPAGLALFTGQNISFYGPVMAGTVISMLPLLVVYIFAQKYF
                     VQGTAMSGMKE"
     gene            complement(23763..24659)
                     /locus_tag="ACG842_000918"
     CDS             complement(23763..24659)
                     /locus_tag="ACG842_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0247480.1"
                     /translation="MNGFTKYWNRPDRAAYLFLTPSFLVLLLFTVIPLLGTFAISFTD
                     LNIFFTSRNFVSFENFTRIFSDERAVNSLIHSLYFTVLETPTQIIVGLLAAVALSKNT
                     RFNRFCRSTFYIPVICSLTSIAIIFSMLLDPNVGAIPYLFSQVGLPIPQFFRDPTLAM
                     PTVAIMTVWKNFGVTMTILLTAIQGISPSLYESAQMDGATNRQQFFNITLPQIVPSLG
                     FCILTNLIGSMQVFDQVYVATAGGPQFKTETAVQYIYSRGFTAPYELGYASALSSVLF
                     IIVATIAISTNVYMSRKEKQMK"
     gene            complement(24834..26123)
                     /locus_tag="ACG842_000919"
     CDS             complement(24834..26123)
                     /locus_tag="ACG842_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFQ0247481.1"
                     /translation="MKEEQKMKGKKWLIGSLLVAATGILGACGGGSSAESDSDKEVTL
                     TFWNGFTASDGEILQEIVNEFNKTNDKNIKIEMDIMTWANLNEKLPTAISSKKAPDFI
                     ALNYPDFAQYVENGAVQPLDDFWEYDGVDKSDFKETAIELGEVEGKQYFVPMQVQGMY
                     MYWNNDLFKQAGLDTEQPPTTWEELTEMAPKLTDSSKNVSGFVFNKDGTAPLYNWMLA
                     NGGKLVNDDYTKSEFASPENLKTLKAIQKMIHVDKTGPESISGAEMDNLMNAGQLAIE
                     INGPWLNNGLKANEIDYNVTTLPQGSDSDKKTAILDGVGYAIPASTDNNKKEAVYEFL
                     KYWNTTEIGKKWSIENGFPAYLDSVAKDEEVKNDPIVSELSKQMDYAEPYLPGFTQIS
                     AINNDIINPLIEKLLAGEDPETLMNDADDAINQLLQK"
     gene            complement(26095..27612)
                     /locus_tag="ACG842_000920"
     CDS             complement(26095..27612)
                     /locus_tag="ACG842_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HFQ0247482.1"
                     /translation="MEATMLISSVKRVGKVDPRLYSSFIEHMGRAVYEGIYQPDHPSA
                     NKSGFRQDVIHLVKELNIPMIRYPGGNFVSGFRWEDSIGPIANRPRRLDLAWRTIETN
                     EVGIHEFYNWCQHVGAEINMAVNLGTRGVDAARNLVEYCNFPKGTYWSDLRRSNGQES
                     PFGIKTWCLGNEMDGPWQIGHKTAEEYGRLAEETAKAMKLVDDSIEVVLCGSSNRQMP
                     TFGDWELTVLDQAYDQIDYLSLHQYYGNQKNDLSHYLARSLDMDRFISEVTAMCDTVK
                     AKKHSKKQINLSFDEWNIWYHSAEQDEQAAPWQIAPPLLEDNYNFEDALLLGCLLITL
                     LKHADRVKIACLAQLVNVIAPIRTETNGEAWRQTIYYPFMQAAVYGQGEVLSPQIRSA
                     NYATEDFEEVPYLESIAVINDKELVIFAVNRGEEEMNLTVHLSELSLEGVIDFSEMSG
                     FDTKATNLPKSEQVKPSHSTNVKAEDGKINCLLKPLSWNVVRCRLSDEGGTKNER"
     gene            27810..28700
                     /locus_tag="ACG842_000921"
     CDS             27810..28700
                     /locus_tag="ACG842_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HFQ0247483.1"
                     /translation="MQLQINEESLPVYEALASKTRIKIIQLLSKKKMNVKDLAKELGV
                     SSAITTMHVKKLEEANIIKTEKVGQQKISSLRVDKIDISFPEKIFNAFDTKETSIPIG
                     HYTNYAIEPTCGLATIHDFIGKVDEPRYFMDPRRMDARILWFTSGFVEYQAPNFLNTE
                     DTLEMLEVSVEISSEFPFSNDNWPSDITFSLNGVELGTWTSPGDFADIRGKYTPDWYP
                     DNLNQYGLLKTIRITKHLTNMNGEPLSNITINDIPKEQDTWHLRIEVKDDAKHVGGCT
                     LFGKGFGNYDQDIKLKVYYS"
     gene            complement(28773..29597)
                     /gene="nadE"
                     /locus_tag="ACG842_000922"
     CDS             complement(28773..29597)
                     /gene="nadE"
                     /locus_tag="ACG842_000922"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HFQ0247484.1"
                     /translation="MSLQTEIINELGVKPTIDPKEEIRKSIDFMKAYLKKYPFLKTFV
                     LGISGGQDSTLAGRLAQLTMEEMREETKDENYQFIAVRLPYGEQADEEDAKAALDFIQ
                     PDVSLRVNIKPAVDAQVQVLSEAGVGISDFNKGNIKARQRMITQYAVAGERAGAVLGT
                     DHAAENITGFFTKFGDGGADILPLFRLDKRQGKQLLQALNAPEKLYTKIPTADLEDGK
                     PMIADEVALGVTYNEIDDYLEGKEVPAQAQEKIEAWWNKTQHKRHLPISVLDDFWK"
     gene            complement(29609..31081)
                     /locus_tag="ACG842_000923"
     CDS             complement(29609..31081)
                     /locus_tag="ACG842_000923"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004516 - nicotinate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HFQ0247485.1"
                     /translation="MNNTYPDDSLTLHTDLYQINMMQTYWELGRADLHAVFECYFREM
                     PFNHGYAVFAGLERLVNYLENLTFNDSDIAYLRGLEVYPEGFLEYLQNFEFKATVRSA
                     REGELVFANEPLIQVEGPLAHCQLVETALLNMVNFQTLIATKAARIKSVIGEDPLLEF
                     GTRRAQELDAAVWGTRAAYIGGADATSNVRAGKIFGIPASGTHAHSLVQSYGNDYEAF
                     MAYAKTHKDCVFLVDTYDTLKSGVPSAIRVAKELGDKINFQGVRIDSGDMAYISKRVR
                     EQLDAAGFTEAKIYASNDLDEATILNLKMQKAKIDVWGVGTKLITAYDQPALGAVFKL
                     VSIEDDEGKMMDTIKLSSNAEKVTTPGKKQVWRITRNFDGKSEGDYVTLWDEDPREEE
                     AIFMFHPVHTFINKTVRDFTARPVLQDIFIEGKRVYELPALNEIKEYTKENLDSLWEE
                     YKRDLNPQKYPVDLSTECWNHKMAIMERMKKSVAELHTEA"
     gene            complement(31718..32080)
                     /locus_tag="ACG842_000924"
     CDS             complement(31718..32080)
                     /locus_tag="ACG842_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HFQ0247486.1"
                     /translation="MKLIETPVNSNLNIKTFYPKVVEFFFGNTAINYYKLFSLDRTQL
                     LLVDTYDKKQVVMINTKKKITRQEIDYAIHHVLKMTREDVKVHVGVKQELERAGIQFK
                     RPNKDIVVIEQKNTKDSI"
     gene            32303..33055
                     /locus_tag="ACG842_000925"
     CDS             32303..33055
                     /locus_tag="ACG842_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFQ0247487.1"
                     /translation="MEYTIKKMSEISGVSPRTLRFYDEIGLLKPARINSSGYRIYGKK
                     EVDRLQHILFYRTMAFKLDDIQEVLDNPSFDHQKALIKHQQMLLEKRAQIDTLLTTVQ
                     QTLDMYEGGRKMSDKEKFEGFKQQKLKENEESYGQEIRQKYGKKKVTASNEKWMNMDK
                     ETYEEMQTVEKQMLFDLTLYLKEPEDESLADRIFQAHKKWLSYSWPEYQPKAHKGLGM
                     MYEADERFTAYYDERSGHGAAKALNEIIQKKA"
     gene            complement(33195..34118)
                     /locus_tag="ACG842_000926"
     CDS             complement(33195..34118)
                     /locus_tag="ACG842_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFQ0247488.1"
                     /translation="MANIRDIAKLTGYSVSTISRVINGHPYVDEEKRKEILAVMKEVD
                     YIPNANARQLSYGKTKSIGVILPYTNHPYFDQLISGIIEAAFDYDYKVTLLPTGYQKE
                     KERRYLEEFAAKAFDGLIITSRANTLDDLLAYQKYGPLVFCEEIKGKQASCVFIDREQ
                     SITEGLTYLKQQGIKQLGITLGRSGRLSYNSKITLQLCHQLFPSFDESLVFWDCIDAE
                     KGKQAGFFFKELGVDGVFTNSDMVAAGILQSYLQDKTPVVIGRDNLLISELLGFPTID
                     HHLEACGEMAFQLFLTEKKDKVKIPYTFIQR"
     gene            complement(34133..34405)
                     /locus_tag="ACG842_000927"
     CDS             complement(34133..34405)
                     /locus_tag="ACG842_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0247489.1"
                     /translation="MNTYVEITDGTTNNYFYAFVEFKGSVLTLYSFQGLNRNIVKEIP
                     MNEIENLTKDIYWGGQRISFSHEGKMYQFFECGPAVVDYLQENLFV"
BASE COUNT        10203 a         7200 c         5955 g        11220 t
ORIGIN      
        1 tatttttcgt ttggttgggc ttattaccgc agatgcaagc ttttgaacac cgtttaagga
       61 agaggttgta aaatcagcgg tttgacctga gtattagaga tacaaaacgg aaaaatagct
      121 tctcatattt tgccgttttt tgagctaata ccgaaggaca gctgattgaa caccgtttat
      181 tcggaaaaag ggactgtgac acgacttttg tcacagcccc taaaaaaaat aataaatttg
      241 tactattcta tgaagcttct tcttcagaaa actgactgtt ataaagatct gcatagaatc
      301 cgtctttttc catcaatgta tcgtggtttc ctgtttctac gatcgaacca tgattcatca
      361 caataatgtt gtctgcatcg cgtattgtag acaagcggtg agcaacaacg aaacttgtcc
      421 ggttttccaa taggcggttc atcgcttttt gaatcagtat ttctgttcgt gtatctacac
      481 tagatgttgc ttcatcaagg atcaggacat ctggatttgc taagaatgcc cgcgcaattg
      541 tgattagctg acgctgccct tgcgaaatat tgcttgcatc ctcgtttaat acagtgttat
      601 atccttctgg caatttacga acaaaatcgt ctacgtgagc agctttagca gcctcaatga
      661 tttgttcgtc tgttgcatcg tcgttcccat atttgatatt gtcatagata cttcctgtaa
      721 acagccaagt atcttgcagt accattgaaa actgtgcgcg caattcatca cgtgaaagat
      781 cacgggtatc tacaccatcg taacgaatac tacctccaga aacatcgtag aaccgttcaa
      841 gaaggttgat cagtgtcgtc ttccctgcac cagtcggacc tacaatagcg accatctcac
      901 caggtttgac atccaagtta aaatcagtca ttagcaagtt atctggtgtg tagccgaatt
      961 gaacattttc aaaacgaact ttgtatggtg tatctttttc ttctggaagg ccagattttt
     1021 cattggacat ctcttcttca tccaacactt caaatacccg ttcggctgaa gcaactgttg
     1081 attggatagt gttcatcaaa cttgcaagtt gagtgattgg ttgagagaac tgtgttgtat
     1141 attgcaagaa cgcttgaaca tcccctaatg tcatgttccc atttgcaact tttactccgc
     1201 ctagtacagc gacgaacaca tagcctagat ttttaacgaa attcatcaac ggcatgatga
     1261 ttgccgaaat aaattgagct ttccaaccag cggcatacag attctgattt tctttttcaa
     1321 atacttcctg gtctttttca gtatgattga atgttttaac aatggtatga ccaccatatg
     1381 tttcttctac ttggttattc aataaaccta aacttttttg ctgatctgcg aaaaacactt
     1441 gtgatttcgg cgctacgatc atcacgatga tcaagcttaa agggatcatc aataacgcga
     1501 ctaacgtcaa ttgccagctg attgaaagca tcatcacgat gaccccaatc aatgtgactg
     1561 cactagttac aaattgagtc aaattttgct gcagcgtgct tgcgatgtta tccatatcat
     1621 tgattgctcg agacatgata tccccatttg tatgtgtatc ataataggaa atcggtacac
     1681 gattcatttt ggcttccaaa tcatgacgta attcataaac tgttcgttgg gaaacacgtg
     1741 tcataataaa ttgctgtaaa aagttgaata cagcagaaat caaatacatc acgattactg
     1801 tcgcaatgat ctgcgcgatt ttatcaaagt ctatcggaaa ttttgttact tgttggcctt
     1861 gttgcatctg ttgagcgcca gccattacac ctttgaatat ttctgtggtt gctttcccca
     1921 agacctttgg tgtttggatt tggaagatca cggctgctat tgctagtaca aatacagcga
     1981 tgatcgcaac catacgttta gacatatagc caaacaatcg gcgtaccgtt ccccagaaat
     2041 ttttaggctt ttcacctttt acaccgagat ttctgccagg tccaccatgt cctggaccgc
     2101 ctgctggtcc atgagaggga cgtgtgtttg ttttactcat gctaagtcct cctctcttag
     2161 ttgtgaatga acgatttctt gataagtttc gtttgttgct aatagttctt catgtgttcc
     2221 tttgccaacc agttttcctt catctagcac aaggatcata tctgcatcca ttactgtact
     2281 tactcgttga gcaactaaaa cggtgatact ttctttcata ttcgttttca atgcttggcg
     2341 aagattggca tccgttttga aatccaacgc tgagaaggaa tcatcaaaca catagatatc
     2401 tgctgttttg actaatgccc gtgcaattgc cagacgctgt ctttgtccac cagagaagtt
     2461 tcctcctcct tgttccacat ggctatctaa gccatcttcc atctctgaaa caaaatcttt
     2521 agcttgtgcg atttctaatg ctttccaaat catttcatct gttgcatccg gcttaccata
     2581 ctgcatattg tctcggatcg ttccagagaa cagaactgct ttttgaggaa caaagcccat
     2641 taattctcgc aaattatatt gagtcatgtc actaatgtcc gttccattta tttggatcga
     2701 accagattcg atgtcatata atcgagggat caaattgact aaggtcgttt tacctgaacc
     2761 agtcccccct atgattgcga cgatctcgcc cgacttggct tggaaatcaa tatcttccaa
     2821 tgcaagtttt tctgctccat gatatcgata attgacatga ttgaaggcca atgttgcgtt
     2881 ttttccagca aaagaaactg ttttaggatt ttcagggtca tggataccga ttttttcatc
     2941 caacacttca ttaatacggt cagctgaagc ttgtgcacga ggcaccatga taaaaatagc
     3001 ggaaagcatc ataaagctga tcaagatctg catcgcataa gtcataaatg caatcagatt
     3061 accgacttcc aaagtcatat ctgctatgta gtgtccacca aaccaagtga ttgctacgtt
     3121 tgtaccgctc atgatcaatg tcatcattgg catcatcaat gcgacgatcg tattaacttt
     3181 gattgccgta tttgtataat ctttattcgc taaatcaaaa cgattttctt caaagcgtgt
     3241 cttatcgaat gctcgaatca ctctgacccc tgtcaatcct tctcgaaata ccaggtttaa
     3301 acggtcagtt ttcttttgca tacttttgaa taatggaaca gcaaagtaca tgattccgcc
     3361 gatcaaaata atcattactg ggatgacaac taagaagatt tttgtcaact gatgatcttt
     3421 ttgataagca agtatgctcg cgccaatcaa cgtgattggt gcattgatca tcatacgtaa
     3481 aaacatttgt gtaaccattt ggatttgagt cacatcattg gtcgttcttg tgatcaaaga
     3541 ggccgttcca tatttatcaa acgcatcttt tgtcagactc tctgattttt tatagacatc
     3601 cgtacgcaat tgcttaccca gtttctgtga ttcacgagta gcaaaaaatg tatttgcgat
     3661 agcagctaag atactgatca aagaaaagcc gatcatcaca aaacctgtat gccaaatata
     3721 atcgacatct ccttgcgcta cccctttatc aataatattg gaggtaagtg tcggcaaata
     3781 taaatctgcc agtacttgga tcaccatgaa aactgcagct gcaattgctg aagtcaagga
     3841 gatccgtttt acgattttta gcatctagtt cccttctttc tatttatata ctccgttttt
     3901 cagccaagaa agtttcaaat tgaattcact gattgcttgc tctactgagt aatcttcaga
     3961 tgttgaaaat aagcgatacg aatgcgcaat tgttgtaaat acagtattca tcactaattg
     4021 gatcagtaat tccaattcat gatcattttt tactttcagc ttcgtccgat cgatcaattc
     4081 aagcaactct tttgcatctt gtttgtgttc tttatgtgga tcgtgaggcg ttttttggcg
     4141 actcgtatga ggagcgactt ttcttgaggc acggtaatcc atgttcataa acaagttacg
     4201 ataaaaagaa gcattctcgc cttccaaaat ctcataaatc attttagaaa aataggattc
     4261 gaatcctttg aatagatcac catcggattc tttcattagt tgtaacatat ctttagagct
     4321 gtcacgtctc attgtctcaa agtaataaaa ataaagatcc tctttatctt caaaatactg
     4381 atagaaactg cctctaggga tatccgctaa tttgacgata ttagcaatcg aagcttcttt
     4441 taagggtact ctagaaaact cgattctcgc agcctcgatt agtctttttt gtttttcttt
     4501 aggcaaatgg aaaaatgtct gtgtcggcat atggatcctc ctttctttca agatgacaca
     4561 ttgtcatatg acgctgtgtc attataacac ctcctacctg ttttgcaaga catttgcata
     4621 gtttttttca ttatcatccg atacattaaa aaagtgataa gaggaatcct tccatttgaa
     4681 ttaatgagag ttgcccatta aaatagccac atactaaaca aaggatgaga taaatggccc
     4741 aatcaattag tgtccaagac ttcaataaat tgcctctcga tgctgacagc atcgttttag
     4801 atacaagaaa taccttagat tacacaaaaa accacttaag ccactcgctt tctatccccg
     4861 ctgctatctt gccaagacaa ttaaaaaatc tagatccaaa gaaactttat tatattctaa
     4921 gctatacagg caggcgatca gaagcattag ccgatcaact tactgctaat ggttttcgtg
     4981 ctgtttatat cattggtggg atggaagctc ttcagtcgag tgttgcttga atattgcttg
     5041 ttagattcaa aagaggctgt gacaaaaaat cgtgtcacag cctcttttat accctgtctt
     5101 tttttagtta tttattcttc aaccttatca atatgacttg atagcctttt ctttttctca
     5161 tagttataac aatcatagca ataaaaggag aaggtaagca tatggcatct gttttagtag
     5221 tcaaaggtca tcccctaact gcagaagaat cgcgcacagt taaggcattg acgtcttttt
     5281 taacaagtta taaagaaaat catccagatg atgaagtgac tgttttggaa ctttatcgcg
     5341 atgatattcc ggaaatcgat gaagaattat tatcaggttg ggaagcatta cgtgctggtg
     5401 cagaattcac ttccttatca gaaagtcaac aacaaaaaat tgctcgtttc aatgaattga
     5461 ctgatcagtt ccttgctgct gataaaatcg ttatcgcaaa cgccttatgg aatctaaata
     5521 tcccaacaaa attaaaagct tggttcgata ccgtcaatgt agctggaaaa acgttccgct
     5581 atacagaaaa cgggccagaa ggattagtga caggtaaaaa agcactgcac atccaatcta
     5641 atggcggtgt atacaacgga caagactttg cctctcaata tgtaaaaggt attttaaact
     5701 ttgtaggcat cgatcaagta gatcagctat tcatcgaagg aatcgattat gaccctgacc
     5761 gtgcagatga gttgatgcaa aatgcattag ataaagctgc tgctttaggt aaatcatttt
     5821 aaaaattgag ataaatgcat acaaaagaga cagaggaaaa aatcttcaaa gcagcatttg
     5881 taccttggat attttcccat ctgtctcttt ttgttattct ttctgctttt tcaacggatt
     5941 ttcttcataa agagaagaga ttgttgtgta ccattcaaaa acatgagtaa tcactacttt
     6001 agctgctgca taaacaggaa tccccaagat cacaccgatg attccgaaca atttcccaga
     6061 agtcagcaga actaataaaa tcgttactgg atggattgct aactgattcc ctagtactaa
     6121 tggagagatc aagcgtcctt ctattgtctg ctctactgca aaaacgatca gtactttgat
     6181 gatcatgaat ggacctgcta caatggctaa aaagatagcc ggaatcatcg ctaaaaatga
     6241 tcctaagtaa gggatcaggt tcaaaaaacc cgcaataatc cccaacgtga cagcatactc
     6301 taaaccaatg accgcaaaac caatcataaa catgattgcc acagcaaaag ccacagttag
     6361 ttgtcctctt atataagaag aaacttgact attcatttca ctcagcacat tcaacgtcgg
     6421 tttttgcatg cgtgtcggta aaaatttgac aaaataaggt gctaattgtt taccgtcttt
     6481 caatagataa aataagataa acggcatcgt cagcagagcc actaaaatag tcgctaccgc
     6541 gccgacaaaa cttccaagac tctgaactgt actcttagat atatcttgga tcatatcgcc
     6601 tgccgaattc atgaacttat cacctgctgt ttccaactgt tcgcgaaatt ggttgaacag
     6661 cgggtcccgc aaaatctcgg ttaatttatt atcaatcgta tctacatatt gagggaagtt
     6721 actgatgaag ctcactgttt gttcctgaat cttagggatg atcactacgc tcccccatgc
     6781 gattagtgca acaaccaaaa caaacagccc aatgatactg taaagccttg gtacttttcg
     6841 cttttcaaag taatcaacta ccggattcat caagtaatac aagattcctg ctaaaatgat
     6901 aggcagacct acgattgcta aaaattgcca gattggtgca aacaaatatg aaacttttgt
     6961 aaataaaaag acgatcagca atattaacaa tacaacaagt aaggctgtga ctacttgatt
     7021 atttaaaaac cagcgccaaa accacgatac cggtctgttc ttcttatttt ttccttccat
     7081 aattcctcct gttagttata aaaaatttgg cttcccaaac caatagcagg cagtctatgc
     7141 ggatgaagat agattccatt tgcaatttgt ccttcttctg gacaagcatc aaaaatcaat
     7201 gatacgtgag ctactgtctc taaattccca ttggccatcg gaccgacata atcgatcgta
     7261 tatacctgat catcaaaagc aattgttccg cctgctttca aagaaaaagg ttcgtctttt
     7321 gatacttctt gaatcaccga atattttcgt aaggcagctg ttgctgtttt atcgaacaaa
     7381 atcagcatgg gttcttcctt gtttattgct tctgtcccaa tttctgtaat ctttgcctga
     7441 atcattgcac tctctccttt aattgctttc aaaaacagta taacacattc tcattcttta
     7501 gccgtgtgga ataattatgt gatagaataa aaacgaaaac tatttcatta caattaggag
     7561 gaatcagctc atatgatcga aaagataatt ttaggaacct atacgcgtcg cgaaagtaaa
     7621 ggaatctata cgatcgacct tgatacagaa aaagaagaat tagccaacct ttcattagct
     7681 gttgaagaaa atagccctac ctatgtagca aaaagtaaag ctggcaatct ttacacagtt
     7741 actagtgtgg atggattagg tggtgccggt gcgtacgata aggattttca ttttttgaat
     7801 gcggtaacag agtctggcgc tcctttgtgt tatgtagcgg ttgatgaagc acgtcaatta
     7861 ctatacgggg cgaactatca taaaggtgaa gtaaatgtct accatatatt aggagatggc
     7921 gaactgaaag cagcagattc aatattccat caagaagcga caggtcctca taaaaaccaa
     7981 gatcatgcgc atgtccacta tacggatctg actcctgacc aacgattagt cgtatgtgat
     8041 cttggtacag atcgtgtgta tacttatgat gtgtccgaaa acggcaaatt gaatcttgta
     8101 actaccttca ctgcagagcc aggaacaggc cctcgccatc ttgtctttca tccaaatgga
     8161 caattcgctt atttatttgg tgaattagac agcacagtcc gcgtgctttc ttataataaa
     8221 gaaacaggtg cttttgaaga attacagaaa atatcgacct tgccagaaga attcgacggt
     8281 gaaaatggag gggcagctat ccgcatttca aatgatggca agttccttta tgcttctaac
     8341 cgaggacata actcgattgc tgtattcgca gtttttgaag atggttccca cattcaaaac
     8401 aagcaaatca tctcaacaga aggggatttt ccccgcgatt tcgcactaga tccaagcaat
     8461 aattttgtcg tgtgtgccaa tcagaacacc gataatctga cattatatcg ccgtcatcct
     8521 gaaacaggat tactggaatt actgaaaaaa gatgtctatg taccagaatg tgtctgtgtt
     8581 tattttgatc gttagtaaga aaaggagaat gaaatgaatc agcaagacgt attagacaga
     8641 ctacgggaag aactaaatat tcctttcttt gatgggaaaa tcgaagataa agagtatagc
     8701 gaagaggaat atcagaaact caaagccgat ttacagaact actttgaaca atatgtacag
     8761 aatgtagaaa actaatcagc tttccatact atctgaaaag aacatcagga caagactttt
     8821 ccgtctatcc tgatgttctt ttctacaaat aaaaaaccgc cgagatggtt agactcgacg
     8881 gaaaaggagt atttactcga ttttgagtaa gtacaaggtt gttgttggtt ataggtatgt
     8941 cattatcata aaggattttg atcactctat cataaaggta atattacatt tatattagat
     9001 aactctagtt tttattgtta tcaaagattt cttttatttt ttcaacaaca aattagtcat
     9061 tttttggatg gcagctgtca attggtctgt cgcttggtct tgttttgctt cagatgtatt
     9121 gaatagatct tcatctaccc ctgattcaag atcgatactt tggatcattt cttcacagcc
     9181 tgctacatat gaaagataag ctttttcaaa atttttatga atgcccattg cttttgctgg
     9241 tggacgcaaa gtactgactt tcttaagcat tgcccgatat ttttttgttc cttcttcaaa
     9301 taacaggatg atctcttcaa gagtcgattt gtccagttca cttgtttttc cttcatcaat
     9361 tgcgtttctg atcacttcat atttcggatg cattccttct ccgattgctt ctgtttcctt
     9421 gacgatttga tcgatggact gcacataata gccaatattc ggtaacatgt tcgttttcct
     9481 actttctcca ttgttgttat tatttattct acttgttttt atcctcaaat gacaagtact
     9541 tgaaaaaatt taatggtttc tatacagctc gccattctta ttcgtgctca aagaaaccat
     9601 ctgctaaact atattgtttc cctgcgacaa tgtcatattg gatcaattga tattcgtcgt
     9661 aaatctcttg ttgcgagcga ttgaattgcg cttctttata ccactgtccg ttttgatagt
     9721 acagttctcg ttcaaacgcc ggaagttcct gttctaatga taaaagcagc tgataaaagc
     9781 catttgttgt ctgattcgtc tgttccatta gattcgctgc aaaataaaac ggactggtga
     9841 ttgcatcgtg agtcgtttgc ttttctaatt ctcctttact gtcgaacata agaaattgtg
     9901 tttcatgcaa tgtttgtttg tcattttttg cttggatcgt atctgaatag atccctggaa
     9961 gatgatcccc ccaaaaaaca actaaagtcc ttctagaaag attcttcaat tcttccgtga
    10021 actgcttcag tgccgtgctg ctataggaaa tatcttgcag ataattctct aagtcaagtg
    10081 tcccacttcc atctgaagtt tttgcaggat agtctagatt atcatacttt ccatcgtagg
    10141 gcatatgtgt ctgcatcgtg actaaatgaa tgaactgagg tgttttttct tccttgagaa
    10201 gtgtcattac ttctttgtaa gctgactcat cagaaatata tggattattc tctatcgtat
    10261 ccgtataggt catcgtacgc tcgctgatga actggtcaaa tcctaatgtc tgataaacat
    10321 cctctctttt atacatcgaa gtattgtatg gatggatcgc tgtcgtctgg tatgattgtg
    10381 cgtttaacgc tgaaacgata gaaggaagct gatccatttt tggtacaagc atcgtgtaag
    10441 gggtggtgag ttgcgcatta aataacgcca tagaaaaacc agtcaatgct tcaaattcga
    10501 tatttgctgt tccaccgcca tagttttgag aaagcatatt gccactatat gtttgatcag
    10561 ctacttcata ataatctgcc aatggctcac cagatacttc tacgccgttt aatcgactag
    10621 gatctgagaa actttcactc atcacgaaca cgatgttcgg cgactcttcc tctacagttt
    10681 tatttttttc atcggctagc ttctggtatt tttcagtgat ttctttgatc ttttctttcg
    10741 aatatccttc aggttcatcc attggttcta ccttcagatt gtacaaaaac ccaccgataa
    10801 aaccagtgtt atagtagttc attttctggc tgtaaggtat ccatagtgcc gttttattat
    10861 atgcttttcg caacaaatta tcgggattgt tgaaattact gatataccct agcagtccaa
    10921 ttgccacaag cagtgtcgtt aatcggatca attggaatcg ccttcctttt ttcaggcttc
    10981 gaaacatgta ccagcaaaac aatcctatca cggttcctgc ggcagctaag atcactgtaa
    11041 acagcatagt gccggtcata tctttcagca atccgatttc agtgatcatc ttcaaatcgt
    11101 ctggataaat cggttcttgc cgataactca ttttcatata atttgcaaaa cctaatacac
    11161 cgatggctac gacatacgta agggaaccta aagggacaga tcctgctagt gcaatcagaa
    11221 aaataagaat aatcagcaag acgagacagg ctaagaagaa tttttccgta tgccaagaaa
    11281 aagcaaattt caacgcaagg tctacggata gatcattttg acaccactgc aaaaataaat
    11341 tactaaaaac aacaagaaaa aagatactga tgattgctgc tgttcctttt aaaatctttt
    11401 tctgtttggt cgaaagtctg cttatatatc ttttcaaatg cttgctcctt catttctatc
    11461 aataaaagaa tgttgcttct cttattttag ttgtacgaaa ttcgaaagta aagaacggaa
    11521 agaagaatta atgaaatcca aatattctct ttctttatgg cctttctggt ttctttaata
    11581 agcggacctt tcgtactcca gaaatttttt ctagctgttg tttcacttga ttgagctgtt
    11641 caaaatcagt actgtctatg tcaatcaata tgtagacatg ctcattctta tggttacgcg
    11701 atatatctgc gatcgctagc tcgttctcat tcagcagaga aaatatctct gctaatatat
    11761 tggttttctt ttgataaaat atagtgaatc gaaacggcgc ttggaaaaac attcgtgcgg
    11821 atggataatt gaccgcttct ctaaccgtac cgaacaaaag ataatctttt aaggcttcaa
    11881 ctgccaaacg tccgctgtct gccaaagctt cctgagtcga tccccctaga tgaggcaaca
    11941 taagtatacg gtcattttct aagaactctt cttctggaaa atcagtcaag tatttagcca
    12001 gatgttgttt ttccaatgct tgcataacag catatttatc aacaatttca cttcttccta
    12061 cattgataag tacagcatct tttttcattt tttctatatt cgcctgatcg atcaatcctt
    12121 ttgtatcttc tgtcaacggc agcatgacta cgatgtaatc cgaggtagaa agaactgttt
    12181 ctaaatctgc ttgccgaaca taatccaact gggcatcgcg gatagaataa cccaagacat
    12241 ccatccctaa gctatagcaa cttagcgcta cttttgtgcc tatcgctcca agaccaagaa
    12301 gaccgatggt tttgccttgc aattctcgtc ccacataagc acttcttttg ttttctgcct
    12361 gttctaaaat attaggacct gttagtgttt gtaccatacg acttgcttcg attatcggac
    12421 gggaagataa gagcaaacag cacaaaacaa gctcttttac tgcattagca tttacacctg
    12481 gtgtattcat gacgatcgtc ccgttttcag aagctttttc aacgttgatc gtgttcaccc
    12541 caacaccagc tcttgaaatt gctagcaaat ctggtgtaag ccattcatct ttcactttcg
    12601 tactacgaat aactattcct tcggctggca aatcatctat tattttgaaa tattcccctt
    12661 gtaacttttc tatctcttgt tctggaatag catcaatcaa atgaacgtga tacataaaac
    12721 cccctcctta ctaacaagta tctcgaaatt tctgcaatta gacaactgtt taccatttga
    12781 cttcctagaa gcaaaaagag cagttagagc agagtaggct gagtgataaa aataaggcta
    12841 tgacaagtta ctgccataag ccttattttc gagagcaaaa tattgagggt gagatatctt
    12901 gataaatggt gttccgttag ctgacattga taataagact aaatttcaaa aaagttgaaa
    12961 agcaaatttc gtcaatctct tcttatttct taaaactaag tggctttttc acaacctctt
    13021 tttatctaat aaccactgtt tccaactcaa ctaattttgc ccacgtttca atttgatctg
    13081 ttgttagatt tagcgagaca acggtatgat gaccgccacc tgcctgaatc catgaatgga
    13141 caccttcatg gaaattcggt ttagttttcc ataacacacg tgcaacaggt aaattaggtg
    13201 cttcagtcgt tggttcaaag gcttctactt cattgatcag cagtttataa tgcgtaccga
    13261 aatctgccat cgatacgact accccttctc ctgctttccc atcaaataca agacgcgccg
    13321 gatcttctct atctcccatt gataaaggag aaacaacgat tcttggtttc gtcgcagcaa
    13381 gcgtcggatc tacttccatc atatgggatt ccaagattgc ttcttggcca ctagctagct
    13441 cgtaagtata atcttccatg aatcctgtat ctttccctcg agccatgatt ttcagcaacc
    13501 gatcaatcgc tgcagttttc cagtcgcctt ccccagcaaa accgtatcct tctgccatca
    13561 aacgttgtac agctagtcca ggtagttgtt tcattccata caggtcttca aaattagtcg
    13621 taaaagcgtt atagcctcct tcttctaaga aacggcgaat accgatttcc tgctgagctt
    13681 gtacttttac atgttcctcc catgtctttt cttcataatc accatagtcg aaggtataca
    13741 attccttgta ctcttcaaat aattgatcga cttcttcgtc tgatacgcga tcgattactt
    13801 gtacaagatc tccgatacca aaataatcaa cagtccaacc aaattgaatc tgtgcttcca
    13861 ctttgtctcc ttctgtgaca cccacgtttc tcatgttgtc accaaaacga gccactttta
    13921 ttccaaaact ttcattatag gctactgcaa catccatcca atcagcaatc tctttttgga
    13981 tctcaggtcg tttccagtag ccaacgacga ttttattttg tttatttaat cgagcattga
    14041 tgaacccata ttcgcggtca ccatgagcgg attgatttaa attcatgaaa tccatatcga
    14101 tcgttttcca aggaatgctt tcgttatatt gcgtagctaa atgaagcaat ggtttttgta
    14161 gaagctttgt cccgcggatc cacatttttg ctggtgagaa agtatgcatc caagtgatca
    14221 cacctgctac ttcttctcga taattgacct ctttcatgat ttttgtgatc gtatcagcag
    14281 taactgctaa ttcttgaagg cggattggat aaggcaattg gccattttca ttcagttcat
    14341 cgaccatttc ttgtgcatgt ttctttactt ctcgcagtgc ttcttcccca tataaatgct
    14401 gtgatcctac aacaaaccaa aattcttttt ctccaatatt caacataatt agccactcct
    14461 tttatttgtt ttgtccgtaa tacgcatttt ttccgtgttt tcgtaaataa tgtttatcta
    14521 aaatccgttg aggcagctct tctgaaaatg aattgagctg tctggtaaat aaattcatct
    14581 tgcatacttc atctagtacg actgcgttca ttaccgcact ttgcgcatct ttcccccaag
    14641 taaatggacc gtgtccgtgt aaaagtaccc ctggaattgc taagggatca attttgcgtt
    14701 ctttgaatgt ttctatgata acttttcctg tttcatattc gtacccgctg tcgatttcct
    14761 gttgtgtcaa aaatcgagca caaggtaccg atccataaaa agtatctgca tgcgtcgtcc
    14821 ccatcgcagg aacatccaat cctgcttgtg cccagatcgt cgcccatgtc gagtgagtat
    14881 ggacgattcc tccgatttga ggaaatgaac gatataaaac agcatgagta ggcgtatccg
    14941 aagagggatt gagtttacct tcaatgatat gattgtccaa gtccacaacg accatgtcgt
    15001 ctgctgtcat ctcttcataa ctgactccgc taggtttgat aacgaaatat cgtgtttcag
    15061 gatcaaaagc gctcacattt ccccatgtat atttgacgag tccgtgttta ggcaagtcaa
    15121 gatttgcttg gtatacttct tctttcagtt gttccagcat tttctatcac tcctttttct
    15181 tgcaattgat gaatcgctgt tttttcaatg tcgatcccct cacggtaacg tctcataaat
    15241 atctgatagc ctgctatttc ttctgctgaa ggtgtgatgg tgattccttg gtcttgtcca
    15301 aacacgtctt ctgccaagaa gttttctaaa gacttcccat gtcgatccat catataggct
    15361 gctaataacg cgattcccca cgctccacct tctccggctg tgtccatcac tgtaactggt
    15421 gcctccatag cagatgctag aatccgctgt ccaacttctg gtgttttaaa aatcccccca
    15481 tgccccacta aacgatcaat ttggacattc tcagatttca atatatccat tccgattctc
    15541 attgcaccaa aagcactggc taaatggatc cgcatgaaat tcgctaaatc aaaacggcta
    15601 cctggtgtac gtacaaataa tggccttcct tcatttactc ctgtaatgtt ttcaccggaa
    15661 aaataaccat aagaaagtaa tcccccgcaa tccgtatcgc cttctagcgc tttgttgaat
    15721 aataccgtga ataatttatc tctgcttatc tccataccca aagaattggt aaattcttca
    15781 aaaatactga cccaagcatt gatatccgaa gagcagttat tcgtatgaac catgcctact
    15841 agttctcctg aaggtgtagt gaccatgtcg atttccggat ggacattttc tagttctttt
    15901 tctaatacga tcatagcgaa ggcggaagta ccagccgaaa tgttccctgt ccgtttggct
    15961 acactgttcg tagcgaccat accggttcct gcatcccctt ctggcggaca aaccgggatt
    16021 cctggtctca aatttcccgt agggtcgatc agataagccc ctgattcgct taacttgcca
    16081 gcttcttctc ctgctactag aactttaggc aaaatgtgat ccaagtcaat cgctacgtgc
    16141 gctttttcga ttaactgacc aaaaatattt ttcatttctt gatggtaggt tttcgttgaa
    16201 ggatcgatag gaaacatgcc tgatgcatct cctacgccta gtactttctc accagtaagc
    16261 tgccaatgga cataacctgc aagcgttgtc aaaaaattaa tttctttcac atgttcttct
    16321 ttattcaaaa tagattgata caaatgagcg atactccatc tctgcggaat attgtattga
    16381 aatttttccg ttaatattcg ttctgcttct tcagtgattg tatttcgcca agtacgaaat
    16441 ggtactagta actgttcctt gctatcaaat gctagataac cgtgcatcat tgcactgaac
    16501 ccaatcgacc cgattgtcgt aagattctcg ccatagattt cgaatacttc tgctgccatt
    16561 tttcggtaac tgacgcgcag tcctttccaa atatcttcta atgaataggt ccagatacca
    16621 ttttctagtt tgttttccca ctcataacta ccagcggcaa taggagtaaa agatgaatca
    16681 atcaatacag ctttgatcct agttgatcca aactcaatcc ctaaagatgt acgaccatct
    16741 atgacatcta acatactttg cgcatttact tccttcataa tgatttaact ccctccttct
    16801 cgtcaaacct gatcaaaccg tctttttagt agcgcttaca atgattattg tatacttttg
    16861 tacgtacatg tcaagcggtt atttacaaga atacaataaa aaacacttgt tttgtacgta
    16921 cataagttat aaaatgaaat tgcttacaaa atctgatcaa ccgccttctc gaaattctag
    16981 aaggagagaa tacttatgaa aaaagaaaaa aaagtttcct catcgtttat ttattttttt
    17041 ggcgcatttg gcggcattct atttggatat gatattggtg ttatgaccgg cgccttacct
    17101 tttttacagc atgattgggg tttggcaggt aaagcttctt tgataggatg gatcacgtct
    17161 tcggtcatgt taggtgcgat ccttggcggc tctttatctg gtcttctttc tgataagctt
    17221 ggacgacgaa aaatgattct tctttctgca cttattttta tggctggttc tgtcctttct
    17281 gcatctgccc ctcacaatgg ttcttatttt ctgattgcag caaggattct tctaggttta
    17341 gctgttggcg ctgcatctgc cctagttcct gcttatatgt ctgaaatggc tcctgctaga
    17401 ctaaggggaa gattgtcagg gatcaatcaa gtgatgattg cttctggcat gctcctttcc
    17461 tatgttgcag attatttgct aaaagtgttg cctgaaacga tggcttggcg cgtgatgtta
    17521 gggctcgctg ctgtccccgc gctgatttta tttttcggtg ttttagctct accagagtca
    17581 ccgcgttttc ttatgcaatc tggccgcttg gaagaggcaa aaagagtatt gaactatatt
    17641 cggacaccaa aagaagctga acaggaattc gaacaaatcc agctgaatgt caaacaagaa
    17701 aagacaaccg gaacttcttg gcatactttg tttttagaaa aatatcgttc cttagtcatt
    17761 gccggtattg gtgtagcagt attccagcaa tttcaaggag ctaatgctat tttttactat
    17821 attccgctga tcgtagaaaa agcgactggg catgcagcga gcgatgcatt gatgtggccg
    17881 atcatccaag gaatcatttt agttgctggt tctttgctct ttttggttat tgcagacaag
    17941 ttcaatcggc gaactttatt gaaaataggc ggtagcgtga tgggactttc attcatccta
    18001 ccagctgttt taggaactgt actggatgct catacaaatt cgttgttgat tctcttgttt
    18061 ttatgtattt atgtcgcatt ttattcttgc acatgggctc cactgacatg ggtaatcata
    18121 ggcgaaatat tcccactagc tgtccgcggg cgagcatctg gcctagcctc ttcttttaat
    18181 tggattggtt ctttcctagt tggcttgctt tttcctgtga tgactgcttc tatgtctcaa
    18241 gaaatcgttt tcggcatttt tgggatcatc tgctttttag gggttttatt tattcaagag
    18301 atcgtaccag aaactcgtgg gaaatcttta gaggaaatcg aacaaagtgc atctaaaaaa
    18361 acttatccta aacgtatttc tatccatcat tcttaaagag gttgtgacag aagtggtcag
    18421 cttcaagaaa taagaaggaa ccgcttagtt tccagtcaca aggaacaatt tcaaaaaaca
    18481 gcttctcatg tttgatgaat attgtgactt gtgaccagtt ggtaggagaa caccgtttag
    18541 tggagaggtg gtgaaagaaa cgttcagcct tgagcgatga caaaagctct cacaaacatt
    18601 gcgacgagta accgtaggag caataagagg cggtaaaaac ccgtctctta tttttcgttt
    18661 tctttcactg tttcccgttc aactagtaca ggttcataga gaatatttgg ctgtgcctgt
    18721 cctttttcaa tcgttgtgac gatccaccgt gcggcatctt gtcccaactt ttcttttgga
    18781 tgcgtcaatg ttgtcaattt tttattccct aaatggctta aagaagaatc gtctgttcct
    18841 acgatcgata ggtcatctgg aatatgatag ccattttctg tcaacttatc tagcattaaa
    18901 ctagctattt gatcgttata gcaaatgatg ccatctaaag cttgttcttc tttcaaccgt
    18961 tgtactgttt tttcagcgat tgtttctcgt gtttccgtgg tgaaagtaat gatatcttcc
    19021 gtcaaaaaac gcacattata ctgttcacac gcgctgataa aacctttcat cctgtatttt
    19081 ccttgaagat cgtcgatttt tgtgatgaac aacagacgct gatgtccttt ttttatcaaa
    19141 tattctgctg ccataaaacc gactttcaca tcatctaaac aaatataaga ggcatttaac
    19201 tcttcataag cagcattgat catcaccatc ggaatgcctt gttctctcaa agcaacataa
    19261 agtgctaagt ttgggttata ctgattactt ttcgtaggct caacgatcaa accatctacc
    19321 ccataagcaa tcattttctt caaacactct ttctccaatt cgtgatcgtt attcgtactg
    19381 gctaaaagca atgaataccc agattctcgt aattcatttt cgattcctcg gataatcgac
    19441 ggaaaaatat aatcagagag atatgtggtg atgacaccga tcgtttttgt tctgttcaat
    19501 ggtgtttttt ctttcttata gctgtcatct acatatgtgc ccgacccttt ttctttacgc
    19561 agatagccct catttaccaa cacagcgatt gcttgccgaa tcgtatgtct gctgacgtca
    19621 tatatttctt gcagcttgaa ttctgaggga atggttgtat ttacttcgta ttcctcacga
    19681 aggatctttt gttttatctc ttctgcaata tcttgatatt ttgccatttg ccttgcccct
    19741 ttttatttgt acgtacatat atcataacga gtctgcaagg tttattcaag caaaaatcaa
    19801 ggtttcttgt gctcataagg catacggaac aggttcacca aattctggaa atccttgctc
    19861 attccacgta aagcattgcg cgttcgcgtg ccgattagga ttgtctaacg gatccccttt
    19921 ttggttcttc tctggtcgag catgatagat caaaatatca aagcacccgt cttctgaaac
    19981 cgtaaacgag ttgtgaccag gaccaaattg cttatttttt tcagaagaaa cgaatactgg
    20041 ctccgataat ttatgccatg attcttttaa taaaagattc ttgtgctcat cagcccacag
    20101 caggcccatg gcatagtttt catctgttgc actaccagaa tacgtaatga atattttccc
    20161 attgcgagta aggactgctg gaccttcatt tactgaaaaa ccccgttttt cccattcata
    20221 ctcaggtata gaaaggagga tctgcttccc ctttaacgtc caaggattat ccatttcgga
    20281 tagataaaga ttagaattcc cagtaatttc aggatcttgt tgggcccaca cataatatag
    20341 ctttccttga gaagagaaag ttgttccgtc taaactgaaa gtttcccttt tcgtttttat
    20401 ttgtcccttt tctatccaat ctgtattcat tggatctata tgctgatttt caagtacgaa
    20461 catgcggtga tgatgagatt catcacgtat agttttatga ttgcttgcag caaagtaaat
    20521 ataccatttt ccattgatat aatggacttc aggtgcccag atcaattcac tcatcggtcc
    20581 ttcttcatga gcctgccata caatcaggct tgcagcatgt tctaattcat tcagtgactt
    20641 tgcccttctt aattcaatca cttggtagcc tggaaccgat cctgtaaaat aataataacc
    20701 atctgtatgt ttatataccc aaggatctgc ccgttgccga acgataggat tattataaag
    20761 tgtcttcatc ataacaaatt tcctcacatt ttctttattc taattacatt ccaagagtat
    20821 ttttctatcc ctacatttaa ttcgtcatgt ttttctattg aaaaatagtt tatttcaggt
    20881 tttatttttt ctggttcttc acgtgtattt ttgtccaaca gatcgctact ggttaatgtg
    20941 tagtgctctc cttgatctag caaattgaaa tcatgttcaa tcgttatctt ttgttgttca
    21001 tttgtgcgat tgatcaagaa aatagtcagc tgattgccgt tatctacgat cacactgtcc
    21061 acataaggaa cattttttat cttttcacaa tcataaacag ggcaaatgat ttgttgtttc
    21121 aatacttgac ctttagcata tttcgataag tgttgcatca cataataagt tggattcctc
    21181 catgcttgtc ctttagcatc tgtcagtatt aaaggtattg tgtttactaa caatgattga
    21241 caactaatct ttacgcgatc tgcgtgtcgt aaaatagcca gccctaatga accaaataat
    21301 aatgtatctt ccatagtaaa gtaacaccaa tcgatgggac taccgacttc aaaatcccta
    21361 tgtctttttt ccggttgggc atgtacattc cattcatcaa acgctagata cattgttttc
    21421 tcacttctca aatatccttt tacataatca caagtagcaa cgatttcttc aatttgacga
    21481 tcgaatcgat aagcttttgc aaggtatgtc gctgtatcat cacgctcatt tttaggaggt
    21541 ttagtcaagt cttcttcatc atacttatca atatagttat gcaaagctaa ataatctaca
    21601 ttctcataag cttcttctaa tgcgatacga tcccattcag ggtacgatgg taaacgtggc
    21661 gtagagcttc caactaagat tgtttcaatt gaatcatcca ctaacttcat gacttttgag
    21721 gcttcatttg ctaacctacc atattcataa gcactcttag cccccacttg ccatggaccg
    21781 tctagctcat tccctaaaca ccataattta acgccaaaag gctttttttg cccatttttt
    21841 attcttaaat cactccagaa ggttcctttt ggaaaattac aatattctac taaatttttt
    21901 gcttcatcaa ttccttttgt tcctaaattc accgtgaata tcggtttcgc ttgtacatga
    21961 ctgatccatt taaaaaattc gtggagtcca aaagaatttg gttctatacc tcgccatgct
    22021 aaatcaattt tttttggtcg ttgttctttc ctacctacag tatccaccca atcataacca
    22081 gatgtaaagt ttcctccagg atagcgaatg acacctaatt ctaattcctt tacataagag
    22141 aggacatctt gtctaaaccc gtcaccatca gctgtttcat ggcccggttc ataaatccct
    22201 gtatagacga cacttcccat atgctcaaca aatgagccaa acagccgaga actgatctct
    22261 cctaccgtaa aatcctctct gatttttact ttcacttctt cgtccaacag ctttccctac
    22321 ctttcatttc ctatagtcaa gataatagat acaagtttcc atacgccacc agcaaagata
    22381 aaaataaaag gagggaaaag aaatatgagc agtacaaatc ctaataacaa tacgcaacaa
    22441 agaatatttc tcaaaaaatc aaaaaatact ttccatgcac tttgttcaaa aatttgtaaa
    22501 aatgtgatat ctttgttttc agaaatcaca tatatccagg ataaaagaaa catggaataa
    22561 ataagtaaaa ctacatatcc actaattgaa agtacgattg tctgttcttc taataattga
    22621 ttaacaagaa aataaataat gatcgttaac agactaagga acaagttctt accaaaatgt
    22681 tgctttattc cttgaaagaa aaaataaatg atatttcctg atccctcacc tttccattgc
    22741 gccattacat aaaaaacagc acatgtagca ggcacgatag taataacagg caaacacacg
    22801 attacccaga gaatacctaa taagatgtaa tcagaaacca caatcaactg tccataaaat
    22861 ttagtctcaa ctattttcat cttcctcact ctttcattcc agacatagct gttccttgaa
    22921 caaaatattt ctgtgcaaaa atataaacca ctagtaatgg cagcattgaa ataaccgttc
    22981 cagccattac aggtccataa aaagaaatgt tctgccctgt aaataaagct aaccctgctg
    23041 gtaaagttct catttttgaa ctacttgtca atatcaatgg ataaagcaag tcattccaac
    23101 tattcatcag cgtgaaaatc cctaatgata aaagagtagg tttgctcaat ggcaacatga
    23161 ttttcaaaaa aatattaaac tcacttaatc catcaatgcg tgctgcctct tccaaagaat
    23221 ctggtaaagt aataaaaaag gaacgcatca tgaagatacc aaatgcagtt gtcatacgag
    23281 gaatgatcaa acctgcataa gtatcaagca tgccaagcaa attgacttca ataaatagag
    23341 gaatcatgaa gacttggaat gggatcatca ttgtcagcaa aacgaaatag aacaaaactg
    23401 attttccttt gaaacgcata cgagcaaatg cataccccgc aagagaatca aagaatacag
    23461 aagtgatcac tgtccctcca gcaaaaatca ctgtattttt gatgtaatct aataaaggga
    23521 tcgttgtcca aaccttaata tagttatcta gggtatagct atctgcaaat attctaggcg
    23581 gaaatgagat aatgtctttt tcgtgtttaa aagaagaaat aatcagccac aaaaaaggaa
    23641 acgcaataac caacaatata actagcatac taatcgttcg aatgattttt ttaccactaa
    23701 ctgcttttcg actattcaca gtaactgtaa tcgagctttc tggtgccata caggtttccc
    23761 tcctatttca tttgtttttc tttccgtgac atgtacacat tagtggagat agctatagtt
    23821 gcaacaatga taaacaaaac cgaagacagt gctgatgcat agcccaattc ataaggtgct
    23881 gtaaatcctc gactgtaaat atattgaaca gctgtttctg tcttaaattg tgggccacca
    23941 gcggttgcaa catatacttg gtcaaacact tgcatcgaac caatcagatt cgttaaaata
    24001 caaaaaccta aggagggaac gatctgaggt agcgtaatgt taaaaaactg ctgtcgattt
    24061 gttgcaccat ccatttgagc agattcatac aaagagggag agattccctg aatagcagtc
    24121 aagagaatcg tcatagtaac accaaaattc ttccaaacag tcatgatggc taccgtcggc
    24181 atagcaaggg ttggatcgcg aaaaaattga gggatcggca agccgacttg tgaaaacaag
    24241 taaggaatcg cccctacatt cggatcaagc aacatagaaa aaataatcgc aattgacgtc
    24301 aatgaacaaa taactggaat gtaaaatgtc gaacgacaaa aacgattgaa tctggtattt
    24361 ttagataatg ctactgctgc taataaacca acgataattt gtgttggcgt ttcgagtacg
    24421 gtgaaatata aagaatgaat caacgaatta actgctcgtt catcactaaa tatacgtgtg
    24481 aaattttcaa aacttacaaa atttcttgat gtaaagaaaa tattcaaatc tgtaaaacta
    24541 atagcaaatg ttcctaaaag cggtatgaca gtaaataata ataaaactaa aaaagatgga
    24601 gttaaaaaca aatacgcagc acgatcagga cgattccaat atttggtgaa tccgttcata
    24661 tagtccacct catctttcat tttgataaaa tatacaatct tggtttaaac gaatctgaat
    24721 aagatatccg aacaatcgct gtgatttttg caataacacc tatctgcaaa tactaggtcg
    24781 atcattcaga tatctgtatc ttgccaacag tctatcctgt ataaagttct gtactatttt
    24841 tgcagtaatt gattgatggc atcatctgca tcgttcatga gtgtctcagg atcttctcct
    24901 gctaataatt tttcaatcag tggattgatg atatcgttat tgatcgcaga gatttgggta
    24961 aaacctggta aatatggctc tgcatagtcc atttgtttcg ataattcaga tacaatagga
    25021 tcatttttta cttcttcatc ttttgctact gaatccaagt aggcaggaaa accattttcg
    25081 atactccatt ttttaccaat ttcagttgta ttccaatatt tcaaaaactc atagacagct
    25141 tcttttttat tattatctgt acttgccgga atggcatatc ctacgccatc taaaatagct
    25201 gtttttttgt ctgaatcact cccttgtggt aaggtagtca cattataatc aatctcgttt
    25261 gctttcaatc cgttatttaa ccatggccca ttgatttcaa tggctagttg accagcattc
    25321 attaaattgt ccatttcggc gccagaaatc gattctggac cagtcttgtc tacatggatc
    25381 attttttgga tcgcttttaa cgttttaagg ttctcaggag acgcaaattc agacttagta
    25441 taatcatcgt tcactaattt accgccattt gctaacatcc agttatataa cggtgcagtt
    25501 ccatccttat tgaagacaaa acctgataca tttttactac tatcagttaa ttttggtgcc
    25561 atttctgtca attcttccca agtagtaggt ggttgttcag tatctaatcc agcttgttta
    25621 aacaaatcat tattccaata catatacatg ccttgtacct gcatggggac aaaatactgt
    25681 ttaccttcta cttcacctaa ttcaatagcc gtttctttaa aatcagattt atctactcca
    25741 tcatattccc agaaatcgtc taatggttgt accgctccgt tttctacata ctgtgcaaaa
    25801 tctggataat ttaatgcaat aaagtcaggt gcttttttag aagaaatcgc tgtaggaagt
    25861 ttttcattca aattggccca agtcatgata tccatttcaa ttttgatgtt tttatcattt
    25921 gttttattaa attcattaac gatttcttgc aatatttcac catcagatgc agtaaaccca
    25981 ttccaaaaag tcaaagtcac ttctttatca gaatcagatt ctgctgaact tccgccaccg
    26041 caagctccta aaatacctgt tgcagctacc aacaaagagc caattaacca ttttttacct
    26101 ttcatttttt gttcctcctt catctgataa acgacatctg accacattcc aagatagcgg
    26161 tttcaataaa caattgattt tcccatcttc agcttttaca tttgttgaat gacttggttt
    26221 tacttgttca cttttaggaa gattagttgc ttttgtatca aatccactca tttcagaaaa
    26281 atcaatcact ccctctaatg ataattcaga caagtgaaca gttagattca tttcttcttc
    26341 tccacggtta actgcaaata tgacaagttc tttgtcattt attacggcta tactttctaa
    26401 atacggcact tcttcaaaat cttccgtcgc atagtttgct gaacgaattt gtgggcttaa
    26461 cacctctcct tgtccataca cagcagcttg cataaatgga taatagatcg tctgtctcca
    26521 agcttctccg tttgtttctg tcctgattgg tgcaataacg tttaccaatt gagcaagaca
    26581 agcaatcttt actctatctg catgtttcaa taaagtgatt aatagacagc ctaatagtaa
    26641 tgcatcttca aaattatagt tatcttctag taaaggagga gcaatctgcc aaggcgctgc
    26701 ttgctcatct tgttccgctg aatgatacca aatattccat tcatcaaagg acaggttgat
    26761 ctgcttctta ctatgtttct tcgccttgac tgtatcgcac atcgctgtca cttcagaaat
    26821 gaaccgatcc atgtctaagg aacgtgcaag ataatgagaa agatcatttt tttggttgcc
    26881 ataatattga tgcagagaca aataatctat ttgatcgtac gcttgatcaa gaactgttaa
    26941 ctcccaatca ccaaatgtag gcatttgccg attcgaactt ccgcataaaa ccacctcaat
    27001 tgaatcatct acgagtttca tcgccttagc cgtttcctct gctaaccgtc catattcttc
    27061 cgctgtttta tgtccaatct gccaaggacc gtccatttca ttccctaaac accatgtctt
    27121 aattccaaaa gggctctctt gaccattcga acgccttaaa tcactccaat aagttccttt
    27181 aggaaaatta caatattcga ctaaattacg agctgcatct actccacgag tacccagatt
    27241 gacagccata ttgatttcgg cccccacatg ctgacaccag ttataaaatt catgaatccc
    27301 aacttcattc gtttcaattg tccgccaagc taaatccaac cttctaggtc tatttgctat
    27361 gggcccaata ctgtcttccc aacgaaatcc tgacacaaaa tttccaccag gatatctaat
    27421 catgggaatg ttcaattctt ttaccaagtg aatgacgtct tggcgaaatc cacttttatt
    27481 agctgatgga tgatctggct gataaatccc ttcatatact gctcgtccca tatgttcaat
    27541 aaatgaactg tacaaccgcg gatcaacttt gcctacacgc ttgacactac taatcaacat
    27601 tgtggcttcc ataactgacc tcctttaatt tatacatgaa taatacacct cgttgaaagc
    27661 gttgtcaata atataaatgt taaacttact tattttttat cgattttaat taaccaaaat
    27721 attaataatg attaaaacaa ataatttaat ataactttta ttaacaaata tgttaatata
    27781 taagaaaaac cttttaagag gtgatatata tgcaactaca aataaacgaa gaatccttac
    27841 cggtatatga agcattagct agtaaaacaa ggatcaagat catccagcta ctttccaaaa
    27901 aaaagatgaa tgtcaaagat ttggcaaaag aacttggagt gagcagtgct atcacgacga
    27961 tgcacgtgaa aaagctagag gaagcaaaca tcatcaaaac agaaaaagta ggacaacaaa
    28021 aaatttcgag ccttcgtgtc gataaaatcg atattagttt tccagaaaaa atattcaatg
    28081 cttttgatac gaaagaaaca tcgataccta ttggtcacta tacaaattat gcaattgaac
    28141 caacatgcgg tttagctacg atccatgatt ttatcgggaa agtggatgaa ccacgctatt
    28201 ttatggatcc tcgaaggatg gatgcccgca ttctctggtt taccagcgga tttgtcgagt
    28261 atcaggcacc taatttttta aatacagagg atacattgga gatgttggaa gtttctgtgg
    28321 aaatttcttc tgaatttcct ttttcaaatg acaattggcc ttcagatatc actttcagcc
    28381 taaatggtgt ggagctagga acatggacga gccctggaga ttttgctgat atccgcggta
    28441 aatatacgcc tgattggtat ccagataatc ttaatcaata cggcttgctg aagacgatcc
    28501 gaattaccaa acacttgacg aatatgaatg gagaaccctt gtcaaacatc acgatcaacg
    28561 acatccctaa agagcaagac acttggcatt tgcgaatcga agtgaaagac gatgccaaac
    28621 atgtgggggg ctgtacttta tttggtaaag gattcggtaa ttacgatcag gatatcaaat
    28681 taaaagttta ctacagctga tatttttctt ttctatttta taaaacaaaa acagccaatc
    28741 cctatgaagg gacggctgtt tttgctttat tgttatttcc agaaatcatc caaaacggaa
    28801 attggcaaat gtcgtttatg ttgtgtcttg ttccaccatg cttcgatttt ttcttgtgct
    28861 tgagcgggaa cttcttttcc ttctagataa tcatcaattt cattatatgt gacacctagt
    28921 gcgacttcat ctgcaatcat tggtttccca tcttcaagat ctgcagtcgg aatttttgtg
    28981 tataattttt ccggtgcatt caatgcttga agcaattgtt tcccttgtcg tttgtccaaa
    29041 cggaataatg gcaaaatgtc ggctccacca tcaccaaatt tcgtgaagaa tccagtaatg
    29101 ttttctgcag catgatctgt ccctaatacg gctcctgctc tttctccagc tacggcatat
    29161 tgcgtaatca tacgttgacg tgctttgata tttcctttgt taaaatcgct gattcccaca
    29221 cctgcttcgc ttaatacttg tacttgtgca tcaacagctg gtttgatatt tactcgcaga
    29281 ctaacatcgg gctggataaa gtcaagagca gcttttgcat cttcttcatc tgcttgttca
    29341 ccataaggaa gacgaacagc gataaattga tagttttcat cttttgtttc ttcacgcatt
    29401 tcttccatcg ttagttgcgc caaacggcct gctaaagtag aatcttggcc gccgctgata
    29461 cctagtacaa aagttttaag aaacggatat tttttcaaat atgctttcat aaagtcaata
    29521 cttttacgta tttcttcttt tggatcaatt gttggtttga cacctaattc attgataatt
    29581 tctgtctgca ggctcattta ttctcctcct atgcttctgt atgaagttct gctacgcttt
    29641 ttttcattcg ttccatgata gccattttat ggttccagca ttcagtagac agatcgactg
    29701 ggtatttttg cggattcaag tctcgtttat actcttccca tagtgaatct agattttctt
    29761 ttgtatattc tttgatttcg tttagtgcag gtaattcgta aactcttttt ccttcgataa
    29821 agatatcttg taaaactggg cgagctgtaa agtctcgaac agttttattg ataaacgtat
    29881 gcactggatg gaacatgaag atagcttctt cttctcgagg gtcttcatcc cataatgtca
    29941 cgtagtctcc ttcagatttc ccatcaaaat ttcgagtgat gcgccacact tgttttttcc
    30001 ctggagtagt tactttttct gcattgcttg ataacttgat cgtatccatc atttttcctt
    30061 catcatcttc gatcgatact agcttgaata ctgcaccaag ggctggctga tcataggctg
    30121 tgatcaattt tgtcccaaca ccccatacat caattttagc tttttgcatc tttaaattca
    30181 aaatcgttgc ttcatccaag tcattcgaag catagatttt tgcttccgta aatccggctg
    30241 catctaattg ttcccgaaca cgcttagata tataggccat atctccactg tcgatgcgta
    30301 cgccttggaa gttgattttg tcacctaatt ctttcgccac gcgaatcgca cttggcacac
    30361 ctgatttcaa cgtatcataa gtgtcgacta agaacacgca gtctttatgg gttttagcgt
    30421 aggccataaa tgcttcataa tcatttccat aagattggac taacgaatga gcatgggtcc
    30481 cgctagctgg tatcccaaat atcttcccag cacgtacatt gcttgttgca tctgctccgc
    30541 cgatatatgc cgctctagtt ccccagactg ctgcatccag ttcttgtgcc cgtctagttc
    30601 caaattccaa tagtggatct tctccgatga cagatttgat acgagctgct tttgtcgcga
    30661 tcaacgtttg gaaattgacc atattcaaca gtgctgtttc tactaattgg cagtgcgcta
    30721 aaggcccttc cacttggatc aatggttcat ttgcaaaaac tagttctcct tcacgagcag
    30781 aacgaactgt tgctttaaat tcaaaatttt gtaaatactc caaaaaacct tcaggataga
    30841 cttctaatcc acgcaagtaa gcaatatcgg aatcattgaa cgtcagattc tctaaataat
    30901 tgactaaccg ttccaatcct gcaaaaaccg cgtatccgtg gttaaaaggc atttctcgaa
    30961 agtaacattc aaaaacagca tgtaaatcag ctcgacctaa ttcccagtat gtctgcatca
    31021 tattgatttg atatagatcc gtatgcagcg tcaaactgtc gtctgggtaa gtattattca
    31081 tcttgtatta ctcctcttgg caagttttca tttcctaccc attataacaa aaaaaattaa
    31141 taggaaaaga aatcttccat tttttctgaa aaagcacatg ttttacggat atttcttgct
    31201 aaaaatgaac tccatctgct aaaactatcc tttcttattg gtatttaacc taaacgagaa
    31261 agaatactta ttttattcaa tgtatccgag acacgaagaa attagcgttt tctatttttc
    31321 ttacttgata aaataagtag attttcctac tacaatcgta gccgttaaga taccgatgat
    31381 tgaacaaata taaccaaatg tctctacaac ataatcaatg gcaaaagttt ttgaagcaat
    31441 aatacgtctt tccttttttg cttcttatcc ccaaagaaac ggatataact tattatgtat
    31501 tgaatttttt tggaaaggcg aattatgaca agatatacct tgcgttgaaa aaactcgcgg
    31561 aaaaaatatc acacgttttt aaaaagatac aaagtaagaa agcagctgtc aatagaatgg
    31621 aaacaaaaat ggcgagacat caatcagtct cgccttgttt tttattgtat acattagata
    31681 tggagcaggt taccataaag atgattatta gtcgttatta aatagaatcc ttcgtatttt
    31741 tttgttctat cactacaata tctttatttg gacgtttgaa ttgtattccg gcacgttcta
    31801 gctcctgttt cactcctaca tggactttga catcttctcg tgtcattttt aaaacgtgat
    31861 gaatcgcata gtcgatttct tgccttgtga tttttttctt cgtattgatc atcacgactt
    31921 gctttttatc atatgtgtct accaatagca gttgtgttcg atctaacgaa aataatttgt
    31981 aataattaat agccgtattc ccaaaaaaga attccactac tttaggataa aatgtcttaa
    32041 tatttaaatt actattcaca ggtgtttcga ttagcttcat ctcattcctc ttttctatat
    32101 agtcgattta gatccaatct taagttatgt taccatcttt cctagcatta aggaatcggt
    32161 tacaattgaa cctttaaaga caaaaggatt taccttttat cacttaatag tataacataa
    32221 tttcatgaga aaaaaataca attttttctt gaccatctcg ttacgtaaag gattatgctt
    32281 acatttgtca ggaggaaaaa taatggagta cacaataaag aaaatgtcag agatatcagg
    32341 agtcagtccg agaaccttgc gcttttatga tgagattgga ctgctaaaac cagctaggat
    32401 caattcttca ggttatcgga tttatggcaa aaaagaagtc gatcgtctgc aacacatctt
    32461 attttatcga acaatggcat tcaagttgga tgatatccaa gaggtcttag acaatccatc
    32521 ttttgaccac cagaaagctt taatcaagca tcagcaaatg ttgctggaaa aacgggcaca
    32581 aatcgatacc ctcttgacaa ctgtccaaca gacacttgat atgtacgaag gaggaagaaa
    32641 aatgagtgac aaagaaaaat ttgaagggtt taaacaacaa aagttgaaag aaaatgaaga
    32701 atcctatgga caagagattc gacaaaaata cggaaaaaaa aaggttacag catccaatga
    32761 aaaatggatg aatatggata aagaaacgta tgaagaaatg caaactgttg aaaagcagat
    32821 gttatttgat ctcactcttt acttaaaaga accagaagat gaaagcttag cagaccgtat
    32881 cttccaagct cataagaagt ggttatctta cagctggcct gaatatcagc caaaagcgca
    32941 taaaggacta ggaatgatgt acgaagccga tgaacggttc actgcatatt atgacgagcg
    33001 aagtggtcat ggtgcagcaa aagctttaaa tgaaattatc caaaaaaagg cgtaatcttt
    33061 tacatgatcc aacaaaaaag tacacgtaaa atgacctaat aacaaaacag cagacaccca
    33121 agttagattt ttatgctaac ttttggtgtc tgctccattc tttcacaagt tttttcatcc
    33181 tactttaaat atattcatcg ttgaataaac gtataaggta ttttcacttt atcctttttt
    33241 tctgtaagaa acaattgaaa tgccatttct ccgcaagctt ccagatggtg gtcgatggtt
    33301 gggaaaccaa gaagctcgct gatcagcaaa ttatcgcgtc caatcaccac tggtgtctta
    33361 tcttgcagat agctttgcaa gatgcctgct gcaaccatat ccgaatttgt aaagacacca
    33421 tcaactccaa gctctttaaa aaagaagcct gcttgttttc ctttctctgc atctatacaa
    33481 tcccaaaaaa caagtgattc atcaaaagat ggaaaaagtt gatgacatag ctgcaaagtg
    33541 atttttgagt tataacttag cctgccactt cgccccaacg tgatgcccaa ttgctttata
    33601 ccttgctgct tcaaataagt aagaccttct gtaattgatt gttctcgatc aataaagaca
    33661 cagcttgcct gttttccctt gatttcttca caaaaaacta gaggtccata tttttgatat
    33721 gccaataaat catctagtgt attggcacga gacgtaatga tcaatccatc gaaagcttta
    33781 gctgcaaatt cttcaagata ccttcgctct ttttctttct gatagcccgt cggtaaaagt
    33841 gtcactttgt aatcataatc aaacgctgct tcaatgatcc cactgatcaa ctgatcaaaa
    33901 tacggatgat tggtataagg cagaatcact ccaatgcttt tcgtttttcc ataacttaac
    33961 tgtctggcat ttgcattagg gatataatcg acttctttca tgaccgcaag tatttctttc
    34021 cttttttcct catctacata tggatgtcca ttgattacac gtgaaatagt agaaactgaa
    34081 taacctgtga gctttgcgat atctcgaatg tttgccactg ctgcttcgcc acctatacaa
    34141 aaagattttc ttgtaaatag tctaccacag ctggaccgca ttcaaaaaac tgatacattt
    34201 ttccttcatg cgaaaaactg atccgctgcc cgccccaata tatatctttt gtcaaatttt
    34261 cgatttcatt cattggtatt tcttttacta tattcctatt caacccttga aaactgtaca
    34321 acgttaatac ggatcccttg aactcgacaa acgcataaaa ataattgttc gttgtgccat
    34381 ctgttatctc tacataagta ttcattgcaa gccctccttt tgatactttg agtatagtgt
    34441 atgaaacgcg tctcatagca aggagtttgt tttgccaaag aaaagtttta ttcctctttc
    34501 attattacca aagtctctta ttttaaattg aatacattat aatgatactg taaatatatc
    34561 tttattatta gtgaatgg
//
LOCUS       DAZKLM010000021        34529 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3764
            SAMEA115706913-rid22582563.denovo.021, whole genome shotgun
            sequence.
ACCESSION   DAZKLM010000021 DAZKLM010000000
VERSION     DAZKLM010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706913
            Sequence Read Archive: ERR13196647
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34529)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34529)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287823.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287823.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,004
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,849
            CDSs (with protein)               :: 2,849
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 41 of 96
            Pseudo Genes (incomplete)         :: 52 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 15 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34529
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706913-rid22582563.denovo.021"
                     /isolate="VRE3764"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            212..604
                     /locus_tag="ACG842_000928"
     CDS             212..604
                     /locus_tag="ACG842_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFQ0247490.1"
                     /translation="MGEKELRRSMSVFPIGTVMKLTDLSARQIRYYEEQDLVHPERSE
                     GNRRMYSLNDIDMLLEIKDYLSEGLNMAGIKRVYEMAQAKKQEAKNKKPLTDHDVRQI
                     FRDEILAQGGLSKTGHYQSQGLQRQFFD"
     gene            632..1972
                     /gene="glnA"
                     /locus_tag="ACG842_000929"
     CDS             632..1972
                     /gene="glnA"
                     /locus_tag="ACG842_000929"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HFQ0247491.1"
                     /translation="MVKKQITGEEIKRIIEEENVRFLRLMFTDILGTIKNVEVPVSQI
                     DKVLENKMMFDGSSIEGFVRIEESDMYLYPDLSTWMIFPWESAHGKVARLICDIYNPD
                     GTPFAGDPRGNLKRALKDMRDLGFTSFNLGPEPEFFLFKLDEDGGITTTLNDKGGYFD
                     FAPTDLGENCRRDIVLELESLGFEVEASHHEVAPGQHEIDFKYADVVDACDNIQTFKL
                     VVKTIARKHGLHATFMPKPLFGINGSGMHCNMSLFNDEGNVFYDKDGELELSETAYHF
                     LGGLLKHARAYTAVCNPTVNSYKRLVPGYEAPVYVAWSGRNRSPLVRVPESRGLSTRL
                     ELRSVDPSANPYLAMAVLLQAGLDGIRNQITPPAAVDRNIYVMDEEEREAAHIQDLPS
                     TIHNAIKELRKDNVMIDALGQHIFSNFVEAKRLEWAAFRQTVSEWEREQYLELY"
     gene            2698..2943
                     /locus_tag="ACG842_000930"
     CDS             2698..2943
                     /locus_tag="ACG842_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0247492.1"
                     /translation="MKEKDCFYDKLYKLLLQVGKSSSLLGGILSIIMFVLFFYLLFHL
                     KFFSNDFLICLGLTLAMFISSLLCGLNFFCTHKGLKK"
     gene            3165..4775
                     /locus_tag="ACG842_000931"
     CDS             3165..4775
                     /locus_tag="ACG842_000931"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HFQ0247493.1"
                     /translation="MTKYIFVTGGVVSSIGKGIVAASLGRLLKNRGLKVTIQKFDPYI
                     NVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDINLNKYSNVTTGKIYSEVLRKE
                     RKGEYLGATVQVIPHITNEIKEKIMRAAKMTDSDVIITEVGGTVGDIESLPFLEALRQ
                     MKADVGTDNVMYIHTTLIPYLKAAGEMKTKPTQHSVKELRGLGIQPNILVVRTEQPVS
                     QSVKNKLAQFCDVEPEAVIESPDVDTLYSIPLVLQAQGMDQIVCDHLKLDTPEADMTE
                     WKELEERVLNLKKKVRIALVGKYVELPDAYISVVEALKHSGFAYNSDIEIDWIKAQEL
                     TRENVEERLKDADGILVPGGFGDRGVEGKIEAIRYARENDVPFLGICLGMQMACVEFA
                     RNVVGLEDASSAETNPDTANNIIDLMADQENIENLGGTLRLGLYPCKIKKGTKTAEAY
                     DGADVVQERHRHRYEFNNKYRQLFEEQGLVFSGVSPDNRLVEIVELPEKKFFVGCQFH
                     PELISRPNRPQKLIKAFVGASLADHETK"
     gene            complement(4752..4964)
                     /locus_tag="ACG842_000932"
     CDS             complement(4752..4964)
                     /locus_tag="ACG842_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0247494.1"
                     /translation="MKMKIFKKFFAKNKISNSALIEKSLLLSIFFPTNNNKSEKEKSD
                     KKIAYPLLLLLFSFYQVQKLFCFVIC"
     gene            5067..5936
                     /locus_tag="ACG842_000933"
     CDS             5067..5936
                     /locus_tag="ACG842_000933"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HFQ0247495.1"
                     /translation="MPVVSGAEFLKAARKGGYAVGGFNTNNLEWTQAILEAAEAKQAP
                     VLIQTSMGAAKYMGGYKICKDLITDLVEAMNITVPVAIHLDHGDYEAALECIELGYTS
                     VMFDGSHLPFEENLKLAKDVVEKAHAKGISVECEVGSIGGEEDGIIGNGELADIEECK
                     QMVATGIDYLACGIGNIHGQYPENWKGLAFDHLQAIADAVGPDMPLVLHGGSGIPQDQ
                     IEKAISMGISKVNVNTEFQLSFAAATRKYIEAGKDKEGKGFDPRKLLAPGKAAIVEDA
                     KSHIDWFGSANKA"
     gene            6101..7372
                     /locus_tag="ACG842_000934"
     CDS             6101..7372
                     /locus_tag="ACG842_000934"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HFQ0247496.1"
                     /translation="MKKIVIKGNRPLAGEVTISGAKNSAVALIPAAILADSPVILEGV
                     PDIQDVHSLIEILEIMGATVHFSNNILEIDPREVVSIPMPHGKINSLRASYYFMGTLL
                     GKFGEAVVGLPGGCYLGPRPIDLHVKGFEALGAEVTNEHGAMYLRTENKGLRGNRIFM
                     DVVSVGATINVMLAAVKAKGQTVIENAAREPEIIDVATLLNNMGAKVRGAGTDVIRIE
                     GVETLHGCRHFMIPDRIEAGTYLALAAAVGNGIKVKNVIFEHLESFIAKLQEIGVHMK
                     ISEDEIEVYPSKELKPANIMTYPYPGFATDLQQPLTALLLMTTGTSEIIDTIYAKRVN
                     HVPELARMGADITVEGNMIIVNGPNKLHGTEVVASDLRAGACLVIAGLLAEGTTTIYN
                     VDYILRGYDHIIEKLTALGASIEMIEEVGAE"
     gene            7369..8664
                     /gene="rho"
                     /locus_tag="ACG842_000935"
     CDS             7369..8664
                     /gene="rho"
                     /locus_tag="ACG842_000935"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HFQ0247497.1"
                     /translation="MSDYLTMAELENKTLKEVYSYAKEFKIPYYSKMNKKELSLAVIR
                     AQAEKQGFYYMEGILDIVGQDGYGFLRPINYGPSAEDIYISASQIRRFSLRNGDKVAG
                     KARPPKESERYYGLMHVESVNGKDPEEAKERPHFPALTPLYPQRQLQLETTPQRLSTR
                     MIDIFSPIGFGQRGLIVAPPKAGKTSVLKEIANGITENYPEVELIVLLIDERPEEVTD
                     LERSVKGDVVSSTFDLQPQNHTRVSELVLERAMRLVEDKRDVVILMDSITRLARAYNL
                     VVPPSGRTLSGGIDPAALYKPKKFFGAARNIEEGGSLTILATALVDTGSRMDDVIYEE
                     FKGTGNLELQLSRELSERRIFPAIDIKKSGTRKEELLMPSEELEEIWKLRKNMTGDSL
                     EYTEQLIRFLRKTKNNQQFFKEFQDVSFGKKNPTRNLKR"
     gene            8763..9026
                     /locus_tag="ACG842_000936"
     CDS             8763..9026
                     /locus_tag="ACG842_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HFQ0247498.1"
                     /translation="MKENIHPDYHPVVFMDSTTGFKFLSGSTKTSQETVEWEDGNTYP
                     VIRVEVTSDSHPFYTGRQKFTQADGRVDRFNKKYGLKDANAAE"
     gene            9471..9920
                     /locus_tag="ACG842_000937"
     CDS             9471..9920
                     /locus_tag="ACG842_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFQ0247499.1"
                     /translation="MRFDQLIAEELIVFDPSIDSKGSLFERIGDVLKKTNKVRNVQKV
                     VKALFKREEEISTGIEDGFGIPHAKSKYVTKPALCFFHTAKLTDYSGIDEMPIEYIFA
                     IVVPEKSADMHLEILSTLSRRLMNADFRKGIKEATTPADIIRQISQE"
     gene            9977..11395
                     /locus_tag="ACG842_000938"
     CDS             9977..11395
                     /locus_tag="ACG842_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HFQ0247500.1"
                     /translation="MKIVGVTACPTGIAHTYMSAEKLEVTARNLNHQAKFETQGVKTE
                     NQLSEQEIKEADAIILAVDKEIELDRFAGKKVKRVSTSRAIKEPQVVIDEALRDIGVF
                     VVSNEKEPAANEKKPTIYNHFMNGVNYMLPFVIAGGIIIALSFAFGITAADPNSADYN
                     VLAAAFSRIGGDTAFAMMVPALGAGIATSIAGKAGFAPGIVAGLLASTGGSGFLGGMI
                     GGVLAGYICDFLANKISVKKEFSAIYQLIVVPFLSILIIGLLMVFVIEQPITWVLDAL
                     TNWLNSLGNTSGLLFGLLIGIMMAADMGGPINKSTSTFSIGLMSAGVNAPIAACMAAG
                     MTPPLGIALATLLFKHKFTKEERTSGQSCWILGASYITEGAIPFAVSDPIRVIPSLMT
                     GSAVAAGISMAVGVTSMAPHGGIWVMLIPNVMNNLPMYVLAIASGTVTTAFMLGLLKK
                     TVKEKKQDKWTKNENLTNEVEA"
     gene            11395..12255
                     /locus_tag="ACG842_000939"
     CDS             11395..12255
                     /locus_tag="ACG842_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HFQ0247501.1"
                     /translation="MLYTMKELLKVAKENQFAVPAFNICSYDMMKAIIEEAERLEAPV
                     ILEIHPDEIAYLSDEFVAAVRAYAYTSKLPIVIHLDHGGTIEDVLRAIRNGYSSVMID
                     ASLKSFDDNVQITKKVVALAHAVNVSVEAELGTIGNNGSAEGGAETIIYTDPDQAKIF
                     VKETGIDTLAVAIGTAHGLYPKNRTPKLNIDLLKELNQQIDLPFVLHGGSGNPDAEVS
                     EAVKYGVAKVNLSSDLKSVFFNALREILSENPSMYEPNAIYPAANKKVQEVVKHKMEI
                     LNTVGKASLY"
     gene            12429..13202
                     /locus_tag="ACG842_000940"
     CDS             12429..13202
                     /locus_tag="ACG842_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFQ0247502.1"
                     /translation="MLNEVENRQQEIVNYLKNNQFARVTDLIELVNYSEATIKRDLVF
                     LEKSGLIRRVRGGAMLIDNRKIDVPYLMKITKLEEETEKRKIADTASSLIRDDMVLFL
                     DSSTTSLHLVRNLARFDGLQIITNGIVTAAMLSEYTTAKVSVLGGAIVPKRATINGSK
                     AYNDALSYYADLAIMSCRGLDFSQGVTETHEGEAMVKKAFRKQSDRLAVLVTQQKIAH
                     KFMHQAIAFHEIDYLVTSKELTKEQYAKVKEHHIECLYG"
     gene            13349..13714
                     /locus_tag="ACG842_000941"
     CDS             13349..13714
                     /locus_tag="ACG842_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2512 family protein"
                     /protein_id="HFQ0247503.1"
                     /translation="MKHVKALVVKAIMIWAILWVVLTGLYGVSFMDSTIVGVIIVVMI
                     YVLGDLLILRKVGNIAATIADAGSAAVILWLYLYFMNDTVDIWMKVLIPALLIGVAEW
                     FFHKWLLSQGIVPDERKME"
     gene            complement(13862..15070)
                     /locus_tag="ACG842_000942"
     CDS             complement(13862..15070)
                     /locus_tag="ACG842_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFQ0247504.1"
                     /translation="MEKKLFNKYFIGITLINFIVYLIYYLLMVIMAVIAQDTLHASLS
                     QAGFASGIYIIGTLLARLFMGKQLELFGRKFTLRGGAIFYLLSTLAYLYTPTIGALYL
                     VRFLNGFGYGTVSTATNAIVTAYIPASKKGEGINYYGLSTSLAAAVGPFLGMILLNMT
                     DFRFIIWFSIVLVFFVTIACLVFPVKNIPLSEEQKISLRSWNINSFIEKKALFITGIA
                     FLMGLSYSSVLSFLSSYTKVIHLVAAGSFFFVVYALVITFTRPLTGRIFDVKGEQYVM
                     YPSYLFLTAGLFLLSVTTNSVTLLISGALVGLGYGTFMSNGQAVCLKIVREHRISIAL
                     STYFIGLDLGLGVGPYLMGMLKSLTSFRGLYVIAGVIPLICTALYLVNGRKHSHEAEG
                     ELIESLKNEI"
     gene            complement(15089..15367)
                     /locus_tag="ACG842_000943"
     CDS             complement(15089..15367)
                     /locus_tag="ACG842_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0247505.1"
                     /translation="MNYWMNTIINRLETAYQTRFDMKASLVFLNDAYQNSIELIKAVD
                     ENPTNECEEFLNLFMSTRDLFIRQLVDRYPSNYHDVEVQIQKLKAYSA"
     gene            15560..16216
                     /locus_tag="ACG842_000944"
     CDS             15560..16216
                     /locus_tag="ACG842_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HFQ0247506.1"
                     /translation="MDSRVLKEYLDKNDFPIIRKGYHKYLTFEGMEDSHTYILKEGTV
                     KASVISNDGREFNLRYIKDLEIVSLLKDEYSQFIDSPYNIRVESEYAEFYRINRVRFW
                     QNINQDPKLQHYVKEYYRYRLLYSMKKMQQMLLNGKLGAVCTQLYELYDLFGVRTEEG
                     MLIDFSITNEEIAKFCGISTASSVNRILKQLKKDGIITVKDHHIVILNMDLLEDNIIF
                     "
     gene            complement(16658..17455)
                     /locus_tag="ACG842_000945"
     CDS             complement(16658..17455)
                     /locus_tag="ACG842_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HFQ0247507.1"
                     /translation="MMKKFFLFFLLLVSVGWPVATHADTATVNDEAGLFTTDQIQSLE
                     QQAEKLNEKIKGRVLIVTTTSNSEEPRDFADNYLRDAVGNDQNGSVLLLDMGQREIYI
                     STSGNMIDYLTDSRIDSILDDVYDQMTNAAYYNAAKAYLSKSATYVEDGVPGGHYRVD
                     EETGKITRYKVLTTVEIIIAVVLALILSLVFYFVTVSRYQLKSGIYKYPFREKSSIKL
                     TEKTDRLTNSFVTTRRIPKSPPPGSGGGGSTTHSSGGGTFGGGGRSF"
     gene            complement(17452..18636)
                     /locus_tag="ACG842_000946"
     CDS             complement(17452..18636)
                     /locus_tag="ACG842_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HFQ0247508.1"
                     /translation="MDVLTHKCPNCGGPLTFDPKDQKFHCEYCLNIYTEEEVSQYEQK
                     QKEARMAGEESKEEPSPTEDFTFTAQEQLADMNEAERKAVDEAGGFSDTADESADNTA
                     AIDLFLCPNCGAEIVTDATTAATYCYYCHNPVVLSGRLSGEFLPNKVLPFAVEKEEAI
                     NRFLAWTKKKWFVPKAFFNKDQIDKLTGVYFPYWATDAEVDGAMQANGTVIRIWRIGD
                     IEYTETKQFAVSRAGKLSFKELVKNALSKNTQQKMVEGVQPFPLDKAIDFKSQYLAGF
                     QAEKRDIEYEAIKSQIQSELKDYSEKLLKDTANGYTTLTGVRTSASITNEVNNYVLLP
                     VWLVTYRSNDSSKKVYYYAMNGQTGKVSGVLPISQKKLGLTALGIFAGLAILFLIGGY
                     LI"
     gene            complement(18661..19908)
                     /locus_tag="ACG842_000947"
     CDS             complement(18661..19908)
                     /locus_tag="ACG842_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HFQ0247509.1"
                     /translation="MGLIKAATSMVGGGLADQWLEVIEPDNMSDTTVMTKGVKVRKDD
                     KRGSNRKGTEDVLTDGTVVHVYPNMMMLLVDGGKIIDYTAEEGYYTIKNDAAPSMFNG
                     TLKEAIAETFDRFKFGGVTPQKQQVFYINLQEIKGIKFGTSAPLNYFDNFYNAELFLR
                     AHGTYSIHVVDPILFYTNAIPKNKTQVEINDINEQYLAEFLTALQSAINQMSADGQRI
                     SYVPSKSLELSKYMDTALDDSWRELRGMEIVSVAVASISYTDDSVKLINMRNEGAMLG
                     DPSVREGYVQGSIARGMEAAGKNEAGAMTGFMGVGMGMNANGSYLSQAAQNNQEQIKQ
                     QAEKQNQQTAQGNADTWTCPVCGTENSGKFCSNCGAAKPVENVQPKLQMRCSECNEIV
                     DLSNGIPKFCPNCGKPFKGIPVN"
     gene            20123..21868
                     /gene="dnaX"
                     /locus_tag="ACG842_000948"
     CDS             20123..21868
                     /gene="dnaX"
                     /locus_tag="ACG842_000948"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HFQ0247510.1"
                     /translation="MAYQALYRVWRSQRFEDIVGQKAVTQTLKNAIVSHKTSHAYLFT
                     GPRGTGKTSAAKIFAKAINCPNSKDGEPCNECEMCRSITAGTQEDVIEIDAASNNGVE
                     EIRFIRDRANYAPTKAAYKVYIIDEVHMLSTGAFNALLKTLEEPKESVIFILATTEPH
                     KIPATIISRTQRFDFKRINTADIVEHLEFILKESTISYEPAALNVIARSAEGGMRDAL
                     SILDQAISFSEGTITLDDAMQVTGSLTDEMMDSYLSACVKKEVPEALETLNQILAAGK
                     ESRRFLEDMLQYCRDLLMYQQAPNLVEEQSSTIRSTFTELAQTVSADQLFSMIKILSD
                     TQNEIRFTNSAAIYLEVATVKLAQLPSASVPSADVAFADDQIVKQLQQEIKELRNEIK
                     ELRSNTGIENTKVQPVQQTNHKKQTSTYRVPTERVYKVLREATRQHLNQVKEVWEDLL
                     MSLSVTQRAMLKASEPVAAGPTGLVIAFDYEIVCQRAANDEELQLSVHNNLSRMIKDY
                     APDPVYITRESWPELRRNYLTQAQEEGTENSFASEDSEGEIELLPQEQEVPEETLVVK
                     AEELFGSEAVTVVDD"
     gene            21888..22202
                     /locus_tag="ACG842_000949"
     CDS             21888..22202
                     /locus_tag="ACG842_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HFQ0247511.1"
                     /translation="MMRGMGNMQGMMKQVQKMQKEMAEAQELLNKTEFTGKATNELVK
                     AVFTGDRRMKDIQINEEVVDPDDIEMLQDLVVMAVNDAIAQIDVETEKKLGKYAKGLP
                     GF"
     gene            22227..22823
                     /gene="recR"
                     /locus_tag="ACG842_000950"
     CDS             22227..22823
                     /gene="recR"
                     /locus_tag="ACG842_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HFQ0247512.1"
                     /translation="MQYPEPIAKLIDSYMKLPGIGQKTATRLAFYTIDMKDEVVNEFA
                     KSLLSVKRDLHFCSICGNITEEDPCEICRNPSRDRSVILVVEEPKDVMAMEKMREYHG
                     LYHVLHGVLSPMEGTGPEDINISSLLQRLHDADVKEVIIATNATTEGEATAMYLSRLI
                     KPAGIKVTRLAHGLSVGSDIEYADEVTLLKAVEGRREL"
     gene            22887..23531
                     /gene="tmk"
                     /locus_tag="ACG842_000951"
     CDS             22887..23531
                     /gene="tmk"
                     /locus_tag="ACG842_000951"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HFQ0247513.1"
                     /translation="MNGLFITIEGPDGAGKTSILNELYPLLKEVAKTEIIQTREPGGI
                     PIAEEIRAVILDPKNDRMDERTEALLYAAARRQHLVEKVLPALAKGKIVLCDRFVDSS
                     LAYQGAGRRIGVTEIARLNEFATEGTTPDFTLYLDVDSDTGLRRIKKNRQNQIDRLDS
                     EGLEFHQRVRHAYLKLAEENPERIHKVDARKSFEEVLQTSYHTIIEQYPQFFEN"
     gene            23546..23875
                     /locus_tag="ACG842_000952"
     CDS             23546..23875
                     /locus_tag="ACG842_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP receptor"
                     /protein_id="HFQ0247514.1"
                     /translation="MKIILAIIQDKDSNRLSNELIDANIRATKLSSTGGFLKAGNSTF
                     IVGIEDDRVEEALEIIKKTCESRKQFVSTPVTLDISMDGGVPYPVEVEVGGATVFVLP
                     VEGFHQF"
     gene            23889..24827
                     /gene="holB"
                     /locus_tag="ACG842_000953"
     CDS             23889..24827
                     /gene="holB"
                     /locus_tag="ACG842_000953"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HFQ0247515.1"
                     /translation="MNQEINLAQMQPIVYQQLQRSFEHGRIAHAYLFEGEKGTGKHEV
                     GIWLAQHLFCTQMKDNLPCGKCNNCQRIEKKEHPDVLVIEPEGQTIKVDQIRRLQTEF
                     SRSGYESRKKVFLIKEAEKMNSSAANSLLKFLEEPPGDFLAVLETDAVGRILPTIQSR
                     CQVLHFQELSKEALIHRLQEEQIPLEKAKLLAFLTNSLGKAVEISQDEWFNDAKDLIY
                     QWFVYLQKQDTQAFIYVQKKLVKSFKEKTQQFTGLSVLMFYYQEAQRQAVANGEYGKV
                     KQINQIIERILIAEQKLRSNVSFQAVAEQFVLHTIF"
     gene            24863..25687
                     /locus_tag="ACG842_000954"
     CDS             24863..25687
                     /locus_tag="ACG842_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HFQ0247516.1"
                     /translation="MVEVVGVRYRDAGHIYYFAPGDSEYVYSDKVLVESQQSLHIATV
                     VLPKKEIDPNDLPEAIRPILHKASQAELEKDKKNKKDAYDALKIAKEKVQLRELEMKM
                     VKAEYTFDRNKLIFSFTADGRIDFRELVKDLAAVFRTRIELRQIGVRDEAKIIGGIGP
                     CGRPLCCSSFLGDFVPVSIKMAKDQSLSLNPTKISGLCGRLMCCLQYESEEYETAKKE
                     LPDFGKVIATPDGKGKVVGLNLLSRIVSIRLTGRENPVEYEWDELKEFIQVGEMNG"
     gene            25680..26027
                     /locus_tag="ACG842_000955"
     CDS             25680..26027
                     /locus_tag="ACG842_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HFQ0247517.1"
                     /translation="MDKRSLYDGLNQLESELRNTLTELVEMKHALHEIVEKNTTLEME
                     NQRLREHLRELNQVAQAPAENVKQELSKSRMNLEKIYEEGFHVCYDLYGSRRDNDEPC
                     AFCLEVIYRESGR"
     gene            26096..26968
                     /gene="rsmI"
                     /locus_tag="ACG842_000956"
     CDS             26096..26968
                     /gene="rsmI"
                     /locus_tag="ACG842_000956"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HFQ0247518.1"
                     /translation="MYNQKSFKGNPVYGDLYLVPTPIGNLEDMSYRSVRMLQESDLIA
                     SEDTRNTQKLLNHFEIHTPQKSLHEHNYKERVPQLIALLKEGKTIAQVSDAGMPSISD
                     PGHELVLACIKEGISVISIPGPTAGMTALIASGLVPQPFLFYGFLPRKKKEQKETLKK
                     LKEQTVTLIFYESPYRITATLTNFLEIFGNRQVVLCRELTKVHEEYLRGSTEELLAYI
                     EEHPVKGECCLLVEGNLFEMPVTEVDEELGTLKEQVEAKIASGEKPNAAIKAVALKNG
                     LKKQEVYRQYHELD"
     gene            27077..28198
                     /gene="dinB"
                     /locus_tag="ACG842_000957"
     CDS             27077..28198
                     /gene="dinB"
                     /locus_tag="ACG842_000957"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HFQ0247519.1"
                     /translation="MMELQFPLHNDTSRKIIHIDMDAFFASVEERDHPEFRGHPLVIA
                     RHPSDTGGRGVVTTANYEARKFGIHSAMSAQKAYELCPQAIFKPGDHHKYSDISKQVR
                     EVFYRYTDIVEPVSIDEAYLDVTENKINCKSAIKIARMIQADIWESVQLTCSAGVSYN
                     KFLAKLASDYQKPRGITVVAPQDAVPFLKALPIEKFHGVGKKTVPRMHELGIYTGKDL
                     YECTEMMLIRNFGKMGYSLYRKVRGIHDSPVNVTRERKSVGKEHTYGQPLQTEEAVLT
                     QLRQLAEKVEEALRRVQKHGKTVVLKVRYTDYSTVTKRVTLPEYIYKKEALFYQASLI
                     WEEILGVEKGIRLLGITLTNLDPMTYENIVLPLWENQEI"
     gene            complement(28252..28854)
                     /gene="nrdG"
                     /locus_tag="ACG842_000958"
     CDS             complement(28252..28854)
                     /gene="nrdG"
                     /locus_tag="ACG842_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HFQ0247520.1"
                     /translation="MRNPKPKEWLAKDYSQNYIADYKPFNFVDGEGVRNSLYVSGCLF
                     ACEGCFNKAAQNFRYGKPFTPELEEQIIADLRNDYVQGLTLLGGEPFLNTDVCLQVVR
                     RIRKEFGTTKDIWSWTGYTFDELLQDSEDKLELLSQIDILVDGRFELSKRDLKLQFRG
                     SSNQRIIDVQKSLESNQVVIWEKCTDATETYEQIKKQDLI"
     gene            complement(29169..31358)
                     /gene="nrdD"
                     /locus_tag="ACG842_000959"
     CDS             complement(29169..31358)
                     /gene="nrdD"
                     /locus_tag="ACG842_000959"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HFQ0247521.1"
                     /translation="MKVNYEEKTVVTQQPETSQMKVIKRDGRHVDFDEQKINEALIKA
                     EKKIHGSLSPLTYEKIQMIADLVVQEIKSRFAENVKIYEIQNIVEHILLEKNEYELAE
                     EYINYRTKRDFARSKATDINFSIEKLINKDQTVVNENANKDSNVFNTQRDLTAGIVGK
                     SIGLKMLPPHIANAHQKGDIHYHDLDYHPYTPMTNCCLIDFKGMLNNGFKIGNAEVES
                     PKSIQTATAQISQIIANVASSQYGGCSADRTDELLAPFAELNYKKHLKDAEEWIDSPE
                     RQKEYAKAKTKKDIFDAMQSLEYEINTLFTSNGQTPFTSLGFGLGENWFEREIQKAIL
                     QIRINGLGSEKRTAIFPKLIFTLKKGVNLNPEDPNYDIKQLALECATKRMYPDILNYD
                     KIVELTGSFKVPMGCRSFLQGWKDENGQEVNVGRMNLGVVTLNLPRIAIESKGDQNKF
                     WQLLSDRLEIMKDALLYRVERCKEAIPANAPILYMYGAFGKRLSRTDSVNELFKNRRA
                     TVSLGYIGLYEVASAFFGGEWETNPEAKAFTLDIVKELKANADAWGDEYGYHFSVYST
                     PSESLTDRFCRLDTEKFGIIENITDKEYYTNSFHYDVRKNPTPFEKLEFEKDYPKYCS
                     GGFIHYCEYPMLQQNPKALEAVWDFAYDKIGYLGTNTPIDHCYQCGFEGDFKPTERGF
                     ECPECGNHDPKTCDVVKRTCGYLGNPQARPMVHGRHKEISSRVKHMK"
     gene            31684..32451
                     /locus_tag="ACG842_000960"
     CDS             31684..32451
                     /locus_tag="ACG842_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0247522.1"
                     /translation="MEKMIEVKHLSKSYGSKLSPVKALDNISFTVEEGEFVGIMGPSG
                     AGKTTLMNILSTINLPTMGSVSIQGKEITKMKNSELSDFRRKKLGFIFQEFNLIDTLN
                     AKDNILLPLAVERMTKEEMEKRVRHVAQLLNIEELLKRYPDELSVGQRQRIAAARALV
                     VQPQVIFADEPTGSLDSKSATELLNYLKTMNQKEKATILLVTHDPYTASYCDRILFIK
                     DGVIFSEVVRRGTRREFFEKVIDMQATIGGGGKMNAV"
     gene            32441..34483
                     /locus_tag="ACG842_000961"
     CDS             32441..34483
                     /locus_tag="ACG842_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0247523.1"
                     /translation="MLYKLAVKSFFRQSRGYLVYFFSLTLSVMIYYSFSAMTYDQSLV
                     RRASQDTSIDGGLSFGGFIITVVLLFFVFSANRFFLNRRQKEIGIYQLFGMNKLQISG
                     IYVLEIMIIGLFACISGILLGIIFSKLFSMILVRMMDMDLTSPFFISIPSVVDTLFAF
                     FIILLAVSVYSIWKIWRYPMIRSFGEKEQADSSTMRFRTRHRLLAVIGLLLLTSGYFG
                     ASHFREITSWMISYHYLDTLVFFFPFLIFFVCVVGTYLFFCYTFRLFLIILSKWKIYY
                     YGINFLIMGNTQVHLLKSWRTNSLITVILGISLMTIGGTASISTILSYQEKVSSPMDY
                     QFDVETEKKIKPILAEEGQKITKEITLHYKVIGSIYSQNLEEPMFQTANLITETQYQA
                     FRKVNPDLPNISLKSPGHTVLLDQMQTLFRNISLYGSSIGLPDNQELKIQQMLPSVLG
                     DETMVYLSPVLIVDDTVFEKAKGVDYQVVNVDVSGGNNEKIDERLSNDLSIKWLNAVY
                     YSYDIVNGSLQGEISTKKLPDDQMKEAQFSQESSRLNFSSSYSKLRAVNRRIGINIFV
                     TLFVGMIVIIATGSILTVRQLAEAEEEKENYHLLTKLGIPQRRIRRMIFEQNALTFFP
                     PMILGITHAVFAINVFTQYIKTADYWLAYLVSGLLILIYLLLYVITSQLYCRIIEE"
BASE COUNT        11104 a         6122 c         7250 g        10053 t
ORIGIN      
        1 aataaaaaca ctaataaagg atagtacagt ataataaaaa ataaaaggta tctggtacga
       61 aaatcaacta gatacctttt ttgtgttctt atttttttgt tattttataa aaaatagaga
      121 tctcatgtta gaaaacctaa catatgcagt tgacaatatt ttttttaatt ggtacacttt
      181 agccaactta taaaacgaaa ggggacgtct gatgggagaa aaggaattgc gccgatctat
      241 gtccgtattt ccaattggta cagttatgaa gctgactgat ttgtctgctc ggcaaattcg
      301 ctattatgaa gaacaagatt tagttcatcc ggaacgaagc gaaggaaacc gacgtatgta
      361 ttcgctgaat gatattgata tgttgcttga gattaaagac tatctttcag agggattgaa
      421 tatggctggg atcaaacgtg tatacgaaat ggcacaggcg aaaaaacaag aagcaaaaaa
      481 caagaagccg ttgacagatc atgacgttcg acaaattttt cgtgatgaga ttttggcgca
      541 aggtgggtta agcaaaacag gtcattatca gtcgcaagga ttgcaaagac aattctttga
      601 ctgaataaat taaaaaagga agagggatac catggtaaag aaacagataa caggtgaaga
      661 aatcaaacga atcattgagg aagaaaacgt acgattttta cgattgatgt tcacagacat
      721 cttaggtacg atcaaaaatg tagaagttcc agtgagccag atcgacaaag tattagaaaa
      781 taaaatgatg tttgacggtt cttcgattga aggatttgtt cgaattgaag aaagcgatat
      841 gtatttgtat ccagatctct ccacttggat gatcttcccg tgggaaagtg ctcatggaaa
      901 agttgctcgt ttaatttgtg atatctataa tccggatggg acaccatttg ccggcgatcc
      961 tcgcgggaat ttgaaacgag cattgaaaga tatgcgcgac cttggtttta cctctttcaa
     1021 tttaggacct gaaccagaat tcttcttatt taaattagac gaagacggtg ggatcacaac
     1081 gaccttgaat gataaaggtg gttattttga ttttgcacca acagatttag gtgaaaattg
     1141 ccgccgtgac atcgtgttgg aattagaaag tctaggcttt gaagtggaag cttcacatca
     1201 tgaagtagcg ccaggacagc atgaaattga ctttaaatat gcagatgtgg tagatgcttg
     1261 tgataatatc caaaccttca agctcgttgt caaaacgatt gcacgcaagc atggacttca
     1321 cgcgactttc atgccgaaac cgttatttgg gatcaatggt tctggtatgc attgcaatat
     1381 gtctttgttc aatgatgaag gaaatgtttt ctatgataaa gacggtgaat tggaactaag
     1441 tgaaacagct tatcatttct taggtggttt gctgaaacat gctcgtgcct acacagccgt
     1501 ttgcaatcca acagtcaatt cttataaacg tctcgttcca ggttatgaag cgcctgttta
     1561 tgtcgcatgg agtggccgta atcgttcgcc attagtacgt gtaccagaat ctcgcggttt
     1621 atccacacgt ttggaattgc ggtcagttga tccatctgca aacccatact tggcaatggc
     1681 agtattgttg caagcaggac tagacggtat ccgtaatcag atcacaccgc cagcagctgt
     1741 tgaccgtaac atttatgtaa tggatgagga agaaagagaa gctgcacata tccaggactt
     1801 accttcaact atccataatg ctatcaaaga attgcgtaag gataatgtaa tgattgacgc
     1861 attaggacaa catatcttct caaattttgt tgaagcaaaa cgtctcgaat gggcggcatt
     1921 cagacaaact gtttccgagt gggaaagaga acaatatcta gaactttatt aaaacaggaa
     1981 taccgtgaat tagaggctgt gaaatcagcg ttttgacctg agtattagag aaactaacgg
     2041 aaaaatagtt cctcatattt tgccgttttt tgagctaata ccgagggtca gctgattgaa
     2101 caccgtttag tggagaggtt gtgaaaaagc tgccctgcaa caagtaataa gaatagccaa
     2161 gcaaaaacag cttttcttgt ttttgaattg gctgaactta ttaccgcaga tgcaagcttt
     2221 tgaacaccgt ttaccaagag gttttgaaaa agacgtttag tttcgagtat taagaaaaat
     2281 tttgtgaaaa tggctttcta catttttgca taaatttggc ttaataccga gaaactggct
     2341 tttgaatacc gtttatccag aggttgtgag cctaccattt aactccgaat cacaagaaat
     2401 aatttcaaaa acagcttctc atgtttgatg gatattgtga cttgtgaccg aggagttggc
     2461 aggagaacac cgtttattta gaaataagga ggcacgactt ttgtcgcatc tccttatttt
     2521 ttacttatgt gattcgttac cttcaatgaa tccctacaag gcacttttgt tgaaaaaaag
     2581 gtcatctttg aaaaaattag tggaattgtt ttgtttttga tattatttaa tagaaaagat
     2641 atgccaaaaa tccgagaact gtggactacg ttttttacgt aaaggaggta aataagggtg
     2701 aaagaaaaag attgtttcta cgataagcta tataaactgt tgctgcaagt aggaaaaagt
     2761 agttctttgt taggtgggat attatctatc ataatgtttg ttttattttt ttatctatta
     2821 tttcacttaa aatttttcag taacgatttt ctgatttgct taggtttaac gttagctatg
     2881 tttatatcaa gcttattatg tgggttaaat tttttttgca ctcataaagg acttaagaaa
     2941 taatttctca aaggatatcg aaaaagaaaa aattgtaagc ggatagtatt catttgagta
     3001 cttgactttc ccttagtcag cttgtattat acaccttggg caccctttta gagggtcaga
     3061 agtttacaga aatttcggct ccctattctt attgaagaat agggagcttt gtttattgtc
     3121 tgagactttt cctgcacata aattaaaaag gagtttttga acttatgaca aaatatattt
     3181 ttgtgaccgg cggtgtcgtt tcatcaatcg gaaaagggat tgttgcagct tctttaggac
     3241 gcttattgaa aaaccgcggc ttaaaagtaa ctatccaaaa atttgatcca tacatcaacg
     3301 tggatccggg gacgatgagc ccatatcagc acggagaagt attcgtaaca gatgatggtg
     3361 ctgaaacaga tttggatctg ggccactacg aacgttttat tgacatcaat ttgaacaaat
     3421 actccaacgt gacgacagga aaaatctatt ctgaagtttt gcgtaaagaa cgtaagggcg
     3481 aatacttggg agcaactgtt caagttattc ctcatattac aaatgagatc aaagaaaaaa
     3541 tcatgcgagc agctaaaatg actgattcag atgtgatcat tacagaagta gggggaacag
     3601 taggggatat agaatctttg ccattccttg aagcacttcg tcaaatgaaa gcagatgtcg
     3661 gtacagataa tgttatgtat atccatacaa cattgattcc ttacttgaaa gctgctggag
     3721 aaatgaaaac taaaccaacg cagcacagtg taaaagagct acgtggttta ggtatccaac
     3781 cgaatatttt ggtggtacgt acagagcaac ctgtttctca aagcgtgaaa aacaaattgg
     3841 cacaattttg tgatgtcgaa ccagaagcag ttatcgagtc gccagatgta gatacacttt
     3901 actccatccc attagtttta caagcacaag gaatggatca aattgtttgc gaccatttaa
     3961 aactagatac accagaagca gacatgacag aatggaaaga attagaagaa cgtgtcttga
     4021 acttgaagaa aaaagtacgg attgcgcttg tcggtaaata tgtcgagtta ccagatgcat
     4081 atatctcagt agttgaagca ttgaaacatt ctggtttcgc ctataattca gacatcgaga
     4141 tcgattggat caaagcacaa gaattaacga gagaaaatgt agaagaacgt ttgaaagacg
     4201 cagatggaat cttggttcct ggtggatttg gtgatcgcgg agtagaaggg aaaatcgaag
     4261 cgattcgtta tgctcgcgaa aatgacgttc cgtttttagg tatttgtctt ggtatgcaga
     4321 tggcttgtgt cgaatttgca cgtaacgtcg ttggattaga agatgcgtca tcagctgaaa
     4381 cgaatccaga tacagcaaac aatattattg atttaatggc tgatcaggaa aacatcgaga
     4441 atttgggtgg aactttacgc cttggattat acccatgtaa aatcaaaaaa ggaacgaaga
     4501 cggctgaagc atatgatgga gccgatgttg tgcaagaacg tcaccgtcac cgctatgagt
     4561 tcaataacaa atatcgtcaa ttgtttgaag aacaagggct agtcttttca ggtgtctcac
     4621 cagataaccg tttagtagaa atcgtagagc tgccagagaa aaaattcttt gtgggatgcc
     4681 aattccaccc agaattgatt tctcgtccaa atcgcccgca aaaattaatc aaagcatttg
     4741 tcggagcatc attagcagat cacgaaacaa aataattttt gaacttgata gaaagagaaa
     4801 agtagcaaga gaaggggata agcaattttc ttgtcacttt tctctttttc tgatttgtta
     4861 ttatttgtag gaaaaaagat agaaagcaaa agactttttt cgattaaagc gctattggat
     4921 attttatttt ttgcaaaaaa cttcttgaaa attttcattt tcattgattt tagtagaaaa
     4981 ctaccgaaat gtttggtaaa atagggtcgt tggttaagaa ttgtcttaac aaatttagtt
     5041 ttacaaaact taggaggaat tttattatgc cagtagtatc aggagctgaa tttttaaaag
     5101 ctgctcgtaa aggcggttac gctgtcggcg gattcaacac aaacaacttg gaatggactc
     5161 aagcaatcct tgaagcggct gaagcaaaac aagcaccagt acttatccaa acatcaatgg
     5221 gtgcagctaa atacatgggt ggttacaaaa tctgtaaaga tttaatcact gatttagttg
     5281 aagcaatgaa catcactgtt ccagttgcta tccacttaga ccatggtgat tacgaagctg
     5341 ctttggaatg tatcgaatta ggctatactt cagtaatgtt cgatggttca catctaccat
     5401 ttgaagaaaa cttgaaatta gctaaagacg ttgttgaaaa agcacatgca aaaggtatct
     5461 cagttgaatg tgaagttggt tcaatcggcg gagaagaaga cggaatcatc ggtaacggcg
     5521 aattagctga tattgaagaa tgcaaacaaa tggttgctac aggtatcgac tacttagctt
     5581 gcggtatcgg taacatccat ggtcaatatc cagaaaactg gaaaggatta gcttttgacc
     5641 acttacaagc aattgctgat gctgtaggtc cagacatgcc tttagtatta catggcggat
     5701 caggtattcc tcaagatcaa atcgaaaaag ctatctcaat gggtatctca aaagtaaatg
     5761 ttaacactga attccaatta tcatttgctg cagctactcg taaatatatc gaagctggta
     5821 aagataaaga aggtaaagga ttcgaccctc gtaaattatt agctccaggt aaagcagcaa
     5881 tcgttgaaga tgctaaatct catatcgact ggttcggttc agctaacaaa gcgtaagcat
     5941 ccacataaca gatacgatat ataggaatgg agaacgactt ttgaagtcgc tctccattct
     6001 tttttactta aaacggcaga ccatgctcgt ttcttttttt tatctatgat aaaatacaag
     6061 catatccatg aaaaaagatt aataaacaag gtggaaacag atgaaaaaaa tcgtaatcaa
     6121 aggaaaccga cctttagctg gtgaagtaac gatcagcggt gcaaaaaata gtgcagtcgc
     6181 attgattcca gctgcaatct tagctgattc cccagtgatc ttggaaggtg tgccggacat
     6241 acaagatgtt cattcattaa ttgaaatttt agagatcatg ggcgcaactg tccatttttc
     6301 caacaacata ctagaaatcg atcctcgaga agtcgtatcg attccgatgc cgcacggaaa
     6361 aatcaatagt ttgcgtgctt cttattattt tatgggaaca ctgcttggta aatttggcga
     6421 agcggtagta ggcctgccag gtggctgtta tttaggccct agaccgattg acctacacgt
     6481 caaaggattt gaagctctag gtgctgaagt gacaaatgaa catggtgcca tgtatttacg
     6541 gacagaaaat aaaggattgc gtggcaatcg gattttcatg gacgtggttt cagtcggagc
     6601 aacgatcaat gtgatgttag ctgctgtaaa agcaaaagga cagacagtaa ttgaaaacgc
     6661 tgcacgagaa ccggaaatca tagatgtggc aactttactg aataatatgg gggcaaaagt
     6721 tcgaggagcg ggtacagatg tgatccgtat cgaaggagtc gaaacattac atggctgtcg
     6781 gcatttcatg attcctgacc gcatcgaagc aggaacctat ctagcattag cagctgcagt
     6841 aggaaatggt atcaaagtga aaaatgtgat ctttgaacat ttggaaagct ttatagcaaa
     6901 attgcaggaa attggtgtcc atatgaaaat cagcgaagat gaaatcgaag tttacccctc
     6961 gaaagaatta aaacccgcaa acatcatgac ctatccttat ccaggatttg ccacagatct
     7021 gcagcagcca cttactgcgt tactattgat gactacaggc acttcagaaa tcatcgatac
     7081 gatctatgcg aaacgtgtga accatgtgcc ggaactagca cgaatgggcg ctgatattac
     7141 cgtggaagga aatatgatca tcgtgaatgg tccaaataaa cttcacggca cagaagtcgt
     7201 ggcttctgat cttcgggcag gtgcttgttt agtgattgct ggactattgg cagaagggac
     7261 aacgacgatt tacaatgtag attatatctt acgcggttat gatcatatta tcgagaaact
     7321 aacagcctta ggggcatcaa ttgaaatgat agaggaagtg ggagctgaat gagcgattat
     7381 ttgacaatgg cagaactaga gaacaaaacg ttaaaagaag tttatagtta tgccaaagaa
     7441 tttaaaattc cttattacag taagatgaat aaaaaggaat tatctctagc tgtgattcgt
     7501 gcacaagcag agaaacaagg attttattat atggaaggaa tattggatat cgttggtcaa
     7561 gatggttacg gttttttacg gccaatcaat tatggaccaa gtgcagaaga tatctatatt
     7621 tctgcctcac aaatcagacg attcagtttg agaaatgggg acaaagtggc aggaaaagcc
     7681 cgtccaccta aagaatcgga acgttattat ggtttgatgc acgtggaaag cgttaatgga
     7741 aaagatccgg aagaagcaaa agaacgtccg cattttccag ccttaacacc tctttatccg
     7801 caaagacagc ttcaactgga aacaacaccg caaagactat ctacccgaat gatcgatatc
     7861 ttttctccaa tcggttttgg tcagcgtgga ttgatcgtcg caccgccaaa agcagggaaa
     7921 acttctgtat taaaagaaat cgcaaatggt atcaccgaaa actatccgga agtagaatta
     7981 atcgttctac tgattgatga acgaccggaa gaagtaacgg atctagaacg aagcgtgaaa
     8041 ggcgacgtgg tatcttctac atttgatctg cagccgcaaa accatactcg agtttctgaa
     8101 ttagtattgg aacgcgcaat gcgtttagtc gaagataaac gagatgtcgt tatcttgatg
     8161 gatagtatta ctagacttgc tcgggcctat aacttagtcg ttccaccaag cggacgtaca
     8221 ttgagtggcg ggattgaccc agccgcattg tataaaccta agaaattttt cggggcagca
     8281 agaaacatcg aagaaggtgg cagtttgacg attttagcga ccgctttagt cgatacagga
     8341 agccgtatgg atgacgtgat ctatgaagaa ttcaaaggaa ctggaaatct agaattacaa
     8401 ctttcacgtg aattatctga acgtcggatc ttcccagcca tcgatatcaa aaaatcaggt
     8461 acccgaaaag aagaattatt gatgccaagt gaagaattag aagaaatttg gaaacttcga
     8521 aaaaatatga ctggagattc attagaatat acggaacaac tgatccgctt tttgcgtaaa
     8581 acaaaaaaca atcagcaatt tttcaaagaa tttcaagatg tctcctttgg taaaaaaaat
     8641 ccgaccagaa atctgaaaag ataattgcaa ccttgactga aacatgttaa gatgttaatg
     8701 ttgtaaatcg acaaatcaac tctggttcag caaatggagc agggcaaagg agcgaaaaaa
     8761 tcatgaaaga aaacatccat ccagattacc acccagtagt attcatggat tcaacaactg
     8821 gtttcaaatt cttgtcaggt tcaacaaaaa cttcacaaga aacagttgaa tgggaagacg
     8881 gtaatacata cccagtcatc cgtgtcgaag taacttctga ctcacatcca ttctacactg
     8941 gacgtcaaaa attcacacaa gcagacggac gtgtggaccg tttcaacaaa aaatacggtc
     9001 tcaaagacgc aaacgctgcg gaataataaa aacgctgata actcaacgtt tagcagacta
     9061 tctgataggg atagtctgtt tttttatgtt ttttgccaat ttaaaacttc gtttgatgct
     9121 gaaaaagtcg taggatcata atgcacaaaa caatgaaaaa ctagtatata gttttaatta
     9181 tccacttgat aaaaacacct tatattaacg aaatgaagag gggttatttc gaagtaaaaa
     9241 accatttacc taaaattaga agctaagctt tcctttcaag gaaggctttt tgttttgtta
     9301 tagacttgcg attgtttgtg agctagatcg ttcattttaa tgacttatac acgcggatga
     9361 tgggtctttt tcctctgttc attcataata ttgaacggta cagctagcga tgctatactg
     9421 acctcagttc caagaaaacg ttaacatttt ttgtggggga gaggtttttt atgaggtttg
     9481 atcaattgat cgcagaagaa ctgattgttt tcgatccatc gatagattca aaaggatcat
     9541 tatttgagcg aataggggat gtgttgaaaa aaacaaataa ggtaagaaat gttcaaaaag
     9601 tcgttaaggc actattcaaa cgagaggagg agatctccac aggaatagaa gatggatttg
     9661 ggattcccca tgcaaaaagc aaatatgtga caaaaccagc actgtgtttt tttcatacag
     9721 ccaaattaac ggactatagc gggatagatg aaatgccaat tgaatatatt tttgccatcg
     9781 tagttcctga aaaatcagca gatatgcatc tagagattct cagcacactc tccagacgat
     9841 tgatgaatgc agattttaga aagggaataa aagaagccac tacgccagca gatatcatca
     9901 gacagattag tcaagaatga aaatagtaga gtctttgtcc gagtatcgat agtgaagaaa
     9961 aaggaggaaa ataatcatga aaatcgttgg tgtaactgct tgcccgactg gaattgctca
    10021 tacttatatg tcggcagaaa aattagaagt aacagcaaga aatctaaacc atcaagcaaa
    10081 attcgaaaca caaggtgtaa aaacagaaaa tcagttatct gaacaagaaa tcaaagaggc
    10141 agatgcaatc attcttgcag tagataaaga aatcgaattg gaccgatttg ccgggaaaaa
    10201 agtaaagcgt gtctctacaa gccgagcaat caaagaaccg caagtagtaa tagacgaagc
    10261 attgagagat attggcgtat tcgttgtgtc taatgaaaaa gaaccagctg caaatgagaa
    10321 aaagccgaca atctataatc atttcatgaa tggtgtgaat tacatgcttc catttgtgat
    10381 cgcaggcgga atcatcatcg cactgagttt tgcctttggg attacagcgg ctgatcccaa
    10441 ctcagcagac tataatgtgt tagcagctgc attttcaagg atcggaggag atacagcgtt
    10501 tgccatgatg gttcctgcgt taggcgcggg gattgctaca tcgattgctg gaaaagcagg
    10561 ttttgcccca ggaattgttg ccggattact ggcctctaca ggtggttcag gttttcttgg
    10621 cggaatgatt ggtggcgtgc tagcagggta tatctgtgat tttctggcaa ataaaattag
    10681 cgtaaaaaaa gaattttctg ctatttatca gctgattgtc gtcccctttt tatcgatctt
    10741 aattattggg cttttgatgg tatttgtgat tgaacaaccg atcacatggg tgttggatgc
    10801 tttgacaaat tggctgaata gcttgggaaa tacatccggg ctattgttcg gattacttat
    10861 cgggatcatg atggcagctg atatgggtgg gccgatcaat aaatcgactt cgactttctc
    10921 gatcggtttg atgtcagctg gagtaaatgc accgattgct gcttgtatgg ctgcaggaat
    10981 gacacctccc ctaggtattg ctttagccac tttattattc aaacataaat ttaccaaaga
    11041 agaacggact tcaggtcaat catgctggat tttaggcgct tcgtatatta cagaaggtgc
    11101 tataccgttt gcggtatctg acccgatccg cgtgatacct agtttgatga caggttcagc
    11161 agtcgctgca gggatttcca tggcagtagg agttacttca atggcacctc atggagggat
    11221 ttgggtgatg ctaattccaa atgttatgaa caatttacct atgtatgtct tggcaattgc
    11281 atcaggtaca gtcactacag cctttatgct tggactattg aaaaaaacag tgaaagagaa
    11341 aaaacaagac aagtggacga aaaatgaaaa tctaacaaat gaggtggaag cgtaatgtta
    11401 tatacaatga aagagttatt gaaagtggct aaagagaatc aatttgcagt accagccttc
    11461 aatatttgca gctatgatat gatgaaagca atcatcgaag aagcagagag attagaagca
    11521 ccagtaatct tagaaattca tcctgatgaa atcgcctatt taagtgatga gtttgttgcg
    11581 gcagtaagag cctatgctta tacaagtaaa ctgccgatcg ttatccactt ggatcatggc
    11641 ggtacgatag aagatgtctt acgggcgatt cgaaacggct attcttccgt gatgatcgat
    11701 gcgtctttga aatcattcga cgacaatgta cagattacga aaaaagtggt tgcgttagct
    11761 catgcggtaa atgtctcagt ggaggctgag cttggaacga ttgggaataa tggttctgct
    11821 gaaggaggtg ctgaaacaat catttataca gatcctgatc aagcgaagat ttttgtgaaa
    11881 gaaacaggaa tcgatacact tgctgtagca attggaacag cgcatggctt gtatccgaaa
    11941 aacagaactc caaaattaaa tatcgactta ctgaaagaat tgaaccaaca aatcgatctt
    12001 ccatttgttt tacatggcgg ttcaggtaat ccggacgcag aagtcagcga agcagtaaaa
    12061 tacggagttg ccaaagtgaa tttgagctca gatcttaaaa gcgtcttttt caatgctttg
    12121 agagaaatat tgagtgaaaa tccatcgatg tacgagccga atgcaattta tccagcagct
    12181 aataaaaagg tacaagaagt ggtcaaacat aaaatggaaa ttttgaacac agtaggcaag
    12241 gctagtttat actaaaaata aaaatgtgtg gtcaagaggg tatgacaata gtcttgtcat
    12301 accctcttaa ccacacacga tgttcagaag tagttttttc gaaaaatttc ttcttatttc
    12361 tcgaagccaa tcacttctgt cacaacctct tttttcctgt tactatataa agtaaatggg
    12421 gtgattttat gttgaatgaa gtggagaata gacagcagga gattgtgaat tatctaaaaa
    12481 acaaccaatt tgcaagagtc accgatttga tcgagttagt aaattatagc gaagccacga
    12541 ttaaacgtga ccttgttttt ctggaaaaaa gtggattgat ccgtagagtt cgcggtggcg
    12601 ccatgcttat tgacaatcga aaaattgacg tgccttacct gatgaagatc accaaattag
    12661 aagaagaaac agaaaaacgt aagattgcag atacagcttc ttctttgatt cgagatgata
    12721 tggtcttatt cttggattcc agtacaacct ctttacactt agtcagaaac ctagcaagat
    12781 ttgatggttt gcagatcatt acgaatggca tcgtcacagc tgctatgttg agtgaatata
    12841 caacggcaaa agtatctgta ttgggagggg caattgttcc gaaacgagca acaatcaacg
    12901 gttcaaaagc ctataacgat gcattgtcat attatgcgga tttagcgata atgagttgtc
    12961 gcgggctaga tttcagccaa ggagtaacag aaactcatga aggagaggca atggtcaaaa
    13021 aagcttttag aaaacaatca gaccggcttg ccgttttagt tacacagcaa aaaattgcac
    13081 ataaattcat gcatcaagcc attgcttttc atgaaatcga ttatctggtg acaagtaaag
    13141 aactgacaaa agaacagtat gcgaaagtaa aggaacacca tatcgaatgt ctgtacggat
    13201 aaattattac gtgaatgttc taagtggctt cctcattttt agataggacc ttatctttgc
    13261 tcgcttctct cagccgatgg tataataatg aaaaggttat cttgttagat aatcttactt
    13321 ttttatccag gagaaaggag tgcagaaaat gaaacatgtg aaggcgttgg tagtgaaggc
    13381 aatcatgatc tgggcgattc tgtgggtcgt gctgacaggt ttatacggtg ttagctttat
    13441 ggattcaacg attgttgggg taattattgt agtcatgatc tatgtgctag gggatctgtt
    13501 gatcttacgt aaagtaggga acatcgcagc gacgatcgca gatgctggtt cagctgcagt
    13561 tatcttgtgg ctctatctat attttatgaa tgatacagtc gatatttgga tgaaagtatt
    13621 gattcctgct ctattgatcg gtgtggcaga atggttcttc cacaagtggt tgctaagtca
    13681 aggcatcgta ccagatgaac ggaaaatgga ataaatcaac agttaaacta ttcttatcga
    13741 taaatgaaag ctttaaaaat aaatagatgt ttaattaaga ataatcataa actattttta
    13801 ctcaaaaaac cgctcagatt attttcagat ctgaacggtt ttttttatct attcatcaag
    13861 atcaaatttc atttttcaat gattcaatca attcgccctc tgcttcatga ctatgttttc
    13921 ggccattaac aagatagagt gctgtacata tcaatggaat gactccggca atcacataca
    13981 atcctctaaa tgaagtaaga gattttagca tccccatcaa ataaggacca acgcctaagc
    14041 ctaggtctaa accaataaag taggtagata aagcaatact gattcgatgt tcgcggacga
    14101 tttttaaaca aacagcctgt ccatttgaca taaacgtacc ataacctagg ccgactaatg
    14161 ctccagagat aagcaatgtc acactattcg tcgttacact cagtaaaaat aatcctgctg
    14221 ttaagaataa atagctagga tacatcacgt attgttctcc tttgacatcg aaaatccttc
    14281 cagtcaatgg acgtgtaaat gtgatgacca aagcatatac gacgaagaaa aatgacccag
    14341 cagcaactaa gtggattact tttgtataag aagataaaaa tgataaaaca cttgaataag
    14401 aaagacccat caaaaaggca attcctgtaa tgaaaagtgc tttcttttca ataaaactat
    14461 tgatattcca tgagcgaaga gatatttttt gttcttcaga taatggaata ttttttacag
    14521 gaaagacaag gcaggcaatc gtaacaaaga agaccagtac aattgaaaac cagataatga
    14581 aacgaaaatc agtcatattc agcaaaatca ttcctaaaaa tggcccaaca gcggcagcta
    14641 aactagtact taatccataa taattgatgc cttccccttt tttagatgca gggatatatg
    14701 cagtaacgat agcatttgta gctgtagaaa ctgtaccgta gccgaatcca ttcaagaatc
    14761 gaactaaata caatgccccg atagttggcg tataaagata tgctaatgtt gaaaggagat
    14821 agaagatcgc gccgcctctt aaggtaaact tccgaccaaa tagttctaac tgtttcccca
    14881 tgaataagcg tgccagcaat gttccgatga tatagatccc agaagcaaaa cctgcttggc
    14941 tcaagctggc gtgtaaggtg tcttgcgcaa tgactgccat gatgaccatc aacagataat
    15001 aaatcaggta gacaataaaa ttgatcaatg tgattccaat aaaatattta ttaaataatt
    15061 tcttttccat gtatgttata cattcccctt aagctgaata tgcttttaat ttttgtattt
    15121 gaacttctac atcgtgataa ttggatggat agcgatcaac aagttggcga ataaataaat
    15181 ctcttgtaga cataaacaga tttaaaaatt cttcgcattc gtttgttgga ttctcatcca
    15241 ctgctttgat caattcgatc gagttttgat acgcatcatt taaaaagact aaagaagctt
    15301 tcatatcaaa tcttgtttga tatgctgttt ctaatcgatt aatgattgtg ttcatccagt
    15361 aattcatgtt ttttccctct attcttaaaa aattttactt ctgcagtata acgaataaaa
    15421 aaaaggaaaa aaatcgcata agtgatggaa aaaggtactt aacaaatgtt aatatcatta
    15481 gtaaaatacc tttggacttt aaaaagtttt aaagtactgt atgatctttt tagatgaaag
    15541 tttggatggg ggagcatcat tggatagtag agtcttaaag gaatatttgg ataaaaatga
    15601 ttttccgatt atacgcaaag gatatcataa atatttgaca ttcgaaggga tggaagattc
    15661 ccatacgtat atcttgaaag agggaacggt aaaagcaagt gttatttcaa atgatgggag
    15721 agaatttaac cttcgctata tcaaagatct tgaaatcgtc tctcttttaa aagacgaata
    15781 ttcacaattc atcgattcgc cgtataatat tcgagttgaa tcagaatatg cagagtttta
    15841 tcgaatcaat cgtgtgcgct tttggcaaaa tatcaaccaa gacccaaagc tacaacacta
    15901 tgtaaaagag tattaccgct atcgtctgct ttattcgatg aaaaaaatgc agcaaatgct
    15961 attaaacgga aagctgggag ctgtttgtac gcaactttac gaattatatg atttattcgg
    16021 tgtgcggaca gaagaaggga tgctgatcga tttttcgatc acgaatgaag aaatcgccaa
    16081 attttgtggc atctcaactg ctagcagtgt aaacagaatt ttaaaacaac tgaaaaaaga
    16141 tggtattatc accgttaaag accatcatat cgttattttg aatatggact tgttagaaga
    16201 caatattatc ttttgatgaa caaagaggtt gtgaaatcag cgttttgacc tgagtattag
    16261 agaaacaaac ggaaaaatag ttcctcatat tttgccgttt tttgagctaa taccgagggt
    16321 cagctgattg aacaccgttt accaagaggt tgtgagcctg ccgtttaact ctgagtcaca
    16381 aggaacaatt tcaaaaaaca gcttttcatg tttgatgaat attgtgactt gtgaccgagg
    16441 agttggtagg agaacaccgt ttaccaagag gttgtgaaaa agctgttctg catcaagtaa
    16501 taagaagagc caagtaaaaa cagtctctta tgtttttaac ttggctggac ttatcaccgt
    16561 agatgtaagc ttttgaacgc cgtttatttg caacaaaagg ggctgtgaca cgatttttgt
    16621 cacagccctt tttttgcgaa cattcattta gcaattttta gaaacttctg ccgccaccgc
    16681 caaaagtccc gccaccgcta gaatgggtag tacttccgcc accgccgctt ccaggaggtg
    16741 ggcttttggg gattcttctt gtcgtgacaa aagaatttgt cagacgatcg gttttttcag
    16801 ttaatttgat actggatttt tctcggaatg gatatttgta gatgcctgat ttcaattgat
    16861 aacgcgaaac agtcacaaaa taaaagacaa gactaaggat aagagccaaa acaacagcaa
    16921 tgatgatttc tactgtcgtc aaaaccttat agcgtgtaat tttgccagtt tcttcgtcta
    16981 cacgataatg tcctcctgga actccgtctt ccacatatgt tgctgattta gacaggtaag
    17041 cttttgcagc attgtaatag gcagcatttg tcatctgatc ataaacatca tctaagatcg
    17101 aatcgattct gctatcagtc aaataatcga tcatattacc agaagtcgaa atgtagattt
    17161 cacgctgtcc catatctaat agcaatactg agccattttg atcatttccg actgcgtctc
    17221 ttaagtaatt atcagcaaag tctcttggtt cttctgaatt agatgtagtc gttacgatca
    17281 agactcgtcc tttgattttt tcattcagct tttctgcctg ttgttccaaa gattggattt
    17341 gatctgttgt gaataaccca gcctcatcat ttaccgtagc tgtatctgca tgagtagcca
    17401 ccggccagcc aacacttact agcaaaagaa agaataaaaa aaattttttc atcatatcaa
    17461 gtaacctccg atcagaaaga ggatcgctag cccagcgaaa atcccaagag cggtcagtcc
    17521 caattttttc tggctgatcg gcaggacacc gctgaccttt cctgtttgtc cattcattgc
    17581 gtagtagtag acttttttac ttgagtcatt gcttcgataa gtaacaagcc ataccggcag
    17641 taatacgtaa ttatttactt cgtttgtgat tgaagcagaa gttcgcacac cggtcaatgt
    17701 tgtatatcca tttgcggtat cttttaacag cttctctgaa tagtctttta attcagattg
    17761 aatctgtgat ttgattgcct catattcgat atctcttttt tctgcttgga acccggctaa
    17821 atactggctt ttaaaatcaa ttgctttatc taacgggaaa ggttgcacgc cttcgaccat
    17881 cttttgttgc gtattttttg ataaggcatt ttttactagt tctttaaaag aaagcttgcc
    17941 tgctcttgaa acagcaaatt gcttcgtttc tgtgtattcg atgtcaccta tccgccatat
    18001 gcggataact gtaccattcg cctgcatggc accatctact tcagcatctg tagcccaata
    18061 agggaaatac acacctgtca acttatcgat ttgatcctta ttgaaaaaag ctttgggaac
    18121 aaaccatttt ttcttggtcc aagcgagaaa tctgttgatt gcttcttctt tctcgacagc
    18181 aaaaggaagt actttatttg gcaagaattc tccgcttaaa cgtcccgata gtacgacggg
    18241 attatggcag tagtaacaat aagtcgcagc agttgtcgca tcagtcacga tctctgcacc
    18301 acagttggga caaagaaaaa gatctatcgc tgcggtatta tctgcagatt cgtccgctgt
    18361 atctgaaaaa ccgcctgcct cgtcaaccgc tttgcgctcg gcttcattca tatcggcaag
    18421 ctgttcttgg gctgtaaaag taaaatcttc ggtaggtgaa ggttcttctt tgctttcttc
    18481 gccagccata cgcgcttctt tttgcttttg ctcatattgg ctgacttctt cttctgtata
    18541 gatattcagg cagtattcgc aatgaaattt ttggtctttg ggatcaaaag tcaacggacc
    18601 gccgcaatta ggacatttat gtgtaagaac atccatcagc tgcacacctt tctaaataaa
    18661 ttaattaacg gggattcctt taaacggttt accacagttt ggacagaatt tagggatgcc
    18721 gttggaaaga tccacgatct cattgcattc actgcatcgc atttgtagct taggctgaac
    18781 gttttctaca ggtttggcag ctccgcagtt ggaacagaat tttccggaat tttctgtccc
    18841 gcaaactgga catgtccaag tatccgcatt tccttgtgca gtttgttgat tttgtttttc
    18901 tgcttgttgt ttgatttgtt cttggttatt ttgagctgct tgagaaagat aagaaccatt
    18961 tgcattcatt cccataccta cacccataaa gccggtcatt gctcctgctt cattttttcc
    19021 ggctgcttcc ataccacgtg caatggaacc ttgcacgtag ccttcacgta cgctaggatc
    19081 acctagcatc gccccttcgt tacgcatatt gatcagtttc acagaatcat cggtgtaaga
    19141 aatgctggct acagccacag aaacaatctc catcccacgt aattcacgcc aagaatcatc
    19201 caatgcagta tccatgtatt tgcttaattc caaactctta gatggtacat aagaaatccg
    19261 ttgaccatct gcagacatct gattgattgc tgattgtagc gctgttagga attcagctaa
    19321 atactgctca ttgatatcat tgatttccac ttgtgttttg tttttaggga ttgcgtttgt
    19381 ataaaataaa atcgggtcaa cgacatgaat agaataagtg ccatgtgccc gtaagaataa
    19441 ttctgcgtta tagaaattgt caaagtagtt caacggcgcg cttgtaccga atttgattcc
    19501 ctttatttct tggagattga tataaaaaac ttgttgtttc tgcggtgtga cgccgccaaa
    19561 tttgaatcgg tcaaaagttt cagcaattgc ttctttcagt gttccattga acatggaagg
    19621 agctgcgtca tttttaatgg tgtaataacc ttcctcagcg gtataatcga tgatttttcc
    19681 accatcgacc aaaagcatca tcatgtttgg atagacatgg acgactgttc cgtctgttaa
    19741 aacatcttct gttcctttac gattggagcc acgtttgtca tctttgcgga ctttgacacc
    19801 ttttgtcatg accgttgtat cactcatatt gtctggttcg atgacttcta gccattgatc
    19861 tgccagaccg ccgccaacca tacttgttgc tgctttgata agacccataa aaaattcctc
    19921 ctctgattca gctagtgcca aacaattatt gtttatttat tataccatag gaagatgtga
    19981 aacttctcgg actagagacg gaattgaaat ggaaaattgc gtattgttta aatacctgaa
    20041 aaagccgaga aggatagtag tttttttacc taatatcacg tataataaga gatagtgaat
    20101 tttttaaaaa agggggatcc ttatggcata tcaagcactt tatcgagtct ggcgttcgca
    20161 gcgtttcgaa gacatcgttg gacaaaaagc tgtgacacag acactaaaaa atgctattgt
    20221 ttcccacaaa acctcacatg cctacttgtt tacaggaccc agaggaaccg gaaagacgag
    20281 tgcagcaaag attttcgcaa aagcaatcaa ctgtccgaac agtaaagacg gtgagccatg
    20341 caacgaatgc gaaatgtgtc gatcaattac tgctggtacg caagaagatg tgatcgaaat
    20401 cgatgctgcc agcaataacg gagtcgaaga aatccgattt atccgtgatc gggcaaatta
    20461 tgcgcctaca aaggcagcgt acaaagtgta cattattgat gaggtacata tgctttcaac
    20521 aggagctttc aatgccttgt tgaaaacatt ggaagagcca aaagaaagtg tcatattcat
    20581 cttagcaacg accgaacctc acaaaatacc agctacgatc atttcacgga ctcagcgatt
    20641 tgattttaaa cggatcaata cggcagatat tgtcgaacat cttgaattta tcttgaaaga
    20701 atcgactatc agctatgaac cagctgcatt gaatgtgatt gcccgttctg cagaaggcgg
    20761 gatgcgggat gcattgagta ttcttgatca ggcaatctcg tttagtgaag gaacaattac
    20821 tttagatgat gccatgcaag tgacaggtag cttaactgac gaaatgatgg acagttactt
    20881 gagtgcttgc gtgaaaaaag aagtaccaga agcattggaa acattgaacc aaattctcgc
    20941 agccggcaaa gaatcacgtc gctttctaga ggatatgtta caatactgcc gtgacttgct
    21001 gatgtatcag caagcaccta acctagttga ggaacaatcg agtacgatca gaagtacatt
    21061 tacagaattg gcacaaactg tttcagcgga tcagctgttt tcaatgataa agattttgag
    21121 cgatactcaa aatgaaattc gttttaccaa tagtgccgct atttatttgg aagtcgctac
    21181 agtcaaactt gcacagctac cgtctgcttc tgttccttca gcagatgtag catttgcaga
    21241 tgatcagata gtaaaacagc tacaacaaga gattaaggaa ctaagaaacg aaatcaagga
    21301 actacgcagt aatacaggaa ttgagaatac aaaagtacag ccggtacagc aaacaaatca
    21361 taaaaaacag acaagtacct atcgtgtacc gacggaacga gtatataaag ttctgcggga
    21421 agcgacaaga cagcatctga accaagtaaa agaagtatgg gaagacttgc tgatgagtct
    21481 atctgtcact cagcgagcaa tgcttaaagc aagcgaacca gttgcagctg gacctacagg
    21541 actagtgatt gcgtttgatt atgagattgt ttgtcaaaga gcagcaaatg acgaagaatt
    21601 gcagctttcg gtacacaaca acttaagccg aatgatcaaa gattatgcgc ctgatcctgt
    21661 ttatattaca agagaaagct ggccagaact tcgaagaaat tatttgacgc aggcacaaga
    21721 agagggaaca gaaaactctt ttgcttctga agactcagaa ggagaaatcg agttgcttcc
    21781 tcaagaacaa gaagtaccag aagaaacact tgtagtaaaa gcagaggaac tgtttggctc
    21841 cgaagctgtt acagtggtag atgattaata aaaaggagta gataattatg atgcgtggaa
    21901 tgggcaatat gcaaggcatg atgaaacaag tacaaaaaat gcaaaaagaa atggcagaag
    21961 cacaagagct gctaaataaa acagagttta caggtaaagc caccaatgaa ttggtcaaag
    22021 cggtttttac aggcgatcgt cgcatgaaag atatccaaat caatgaagaa gtcgttgatc
    22081 ctgatgatat cgagatgctt caggatcttg tcgtaatggc agtcaatgat gcgatcgcac
    22141 aaattgatgt ggaaactgag aaaaaattag ggaaatacgc aaaaggtttg ccaggatttt
    22201 agttttaaac tgaaggagta atgaatatgc aatatccaga accaattgcg aaactgatcg
    22261 acagctacat gaaacttcca ggaatcggtc agaaaacagc gacaagactc gccttttata
    22321 cgatcgatat gaaagatgaa gtagtaaatg aatttgctaa atctttgtta agtgtaaaac
    22381 gcgatttgca tttttgtagt atctgcggaa acatcacaga agaagatccc tgcgaaatat
    22441 gtagaaatcc ttcacgtgat cgaagcgtta tcctagtggt cgaagagccg aaagatgtaa
    22501 tggcaatgga aaaaatgcga gaatatcacg gactttatca tgttctccat ggtgtgttat
    22561 caccgatgga agggacaggt ccagaagaca tcaatatttc ttctctgttg cagcggctgc
    22621 atgatgcaga tgtaaaagaa gtgattatcg caacaaatgc aacaacagag ggagaagcaa
    22681 cagctatgta tctctcacgt ctgatcaaac ccgcaggtat caaagtgact cgtctagcac
    22741 atggtctgtc tgtaggctct gatatcgagt atgcagatga agttacttta ttgaaagctg
    22801 tcgaaggaag acgtgagtta taaatttcta attaagtgtt ttttcagata caatataaaa
    22861 gcagcacaag agatgggggc aacaatgtga acggactatt tattacaatc gaggggcctg
    22921 atggtgcagg aaaaaccagc atcttgaatg aactttaccc tcttttgaaa gaagtagcaa
    22981 aaacagaaat cattcaaaca agagaaccag gaggcattcc gattgctgaa gagatccgag
    23041 cagtgatcct tgatccaaaa aatgatcgga tggatgaacg tacggaagca ctgctttatg
    23101 cagcagcaag aagacaacat ctagtcgaaa aggttctgcc tgctctggca aaagggaaaa
    23161 tcgttttatg cgatagattt gtcgacagtt ctcttgctta tcaaggtgca ggccgacgca
    23221 tcggcgttac cgagattgct cggttgaatg agtttgctac agaaggtact acgccggatt
    23281 ttacccttta tcttgacgta gattcagaca ccggattaag aagaatcaag aaaaacagac
    23341 aaaatcaaat cgaccgttta gattcggaag gtttggaatt ccaccagcgt gtacgtcatg
    23401 cttacttaaa gctggcagaa gagaatccgg aaagaatcca caaagttgat gcaagaaaaa
    23461 gctttgaaga agtgctgcaa acaagttatc atacaattat tgaacagtat ccgcaatttt
    23521 ttgaaaacta ggaaggtgaa cgtttatgaa aattatttta gcgattatcc aagacaaaga
    23581 cagcaaccgt ttatcaaatg aattgattga tgcaaatatc cgtgccacaa aactatcatc
    23641 gacaggcggc ttccttaagg ctggaaacag tacgtttatc gtgggtattg aagatgaccg
    23701 tgtagaagaa gcattggaaa tcatcaaaaa aacgtgtgaa tcaagaaagc aatttgtatc
    23761 tacacctgtt acactggata tttcgatgga tgggggcgtc ccttatccag tagaagtaga
    23821 agtaggcggt gccacagtct tcgtcttacc tgttgaagga ttccatcaat tttaggagga
    23881 gctgagctat gaatcaagaa ataaacttgg ctcaaatgca accgatcgtg tatcagcagc
    23941 ttcaacggag ttttgagcat gggcgtatcg ctcatgctta tctttttgaa ggggagaaag
    24001 gaacaggaaa acatgaagta ggtatatggc ttgcacagca cctgttctgt acccaaatga
    24061 aagacaatct gccttgtgga aaatgcaaca attgtcagcg gatcgaaaag aaagaacatc
    24121 cagatgtttt ggttattgaa ccggaaggac aaaccatcaa agtcgatcag atcagacgtt
    24181 tgcagacgga attcagtcgc agcggttacg aatcaagaaa aaaagttttt ttgatcaaag
    24241 aagctgaaaa aatgaatagc agtgcggcaa acagtttgtt gaaattcttg gaagagcccc
    24301 ctggagattt tttagcggtg ttagaaacag atgcagtagg aagaattctc cctactattc
    24361 aatctcgttg tcaagttctg cattttcaag aactttcgaa agaagcactg atccatcgat
    24421 tgcaagaaga gcagattcca ttggaaaaag caaaactttt ggctttctta acaaacagtt
    24481 taggaaaagc agttgaaatc tctcaagatg aatggtttaa tgatgctaag gacttgattt
    24541 atcaatggtt cgtttacttg caaaagcaag atactcaagc ctttatctat gtccaaaaaa
    24601 aattggtaaa atcatttaaa gaaaaaacac aacagtttac aggattatct gtcttgatgt
    24661 tttattatca ggaagcacag cgtcaagcag tagcaaacgg cgaatatggc aaagtgaaac
    24721 agataaacca gataattgaa cggatattga tcgcagagca gaaactacgc agcaatgtca
    24781 gttttcaggc tgttgcggaa cagttcgttt tgcatacgat tttttagctt gccgcaaagg
    24841 atgatgatca aggaggatgg aaatggtaga agtagtaggt gtccgatata gagatgcagg
    24901 tcatatctat tattttgcac caggagactc tgaatatgtt tatagtgaca aggtattagt
    24961 cgaatctcag caatccttac atattgctac tgttgtcctt cctaaaaaag agattgatcc
    25021 caatgacctt cctgaagcta tccgtccgat cttgcataaa gcgtcacaag ctgaattaga
    25081 aaaagacaaa aaaaacaaaa aagatgctta tgatgcattg aaaatcgcaa aagaaaaagt
    25141 gcagttgcgt gagttggaaa tgaaaatggt caaagccgaa tacacatttg atcgaaataa
    25201 attgattttt tctttcacag cggacgggcg aattgacttt agagaattgg taaaagatct
    25261 ggccgctgta tttcgtactc gtattgagct tcgacaaatt ggtgtccgcg atgaagcaaa
    25321 gataataggc ggaattggtc catgtgggcg tccattatgc tgttcttctt ttttaggcga
    25381 tttcgtgcct gtatcaatca aaatggcaaa agatcaaagt ttatctttga atccaacaaa
    25441 aatttccggt ttatgcggac gcttgatgtg ctgtctgcaa tatgagagcg aagaatacga
    25501 gacagcgaaa aaagaactcc ctgactttgg aaaagtaatt gctacacctg atggtaaagg
    25561 gaaagtcgta gggttgaatc tgcttagccg tatcgttagt attcggctga ctggacggga
    25621 aaatcctgtc gaatatgaat gggatgaatt gaaagaattt atccaagtag gtgagatgaa
    25681 tggataagag atcattatac gacggattga atcaattaga atcagagttg agaaatacat
    25741 taacagaact tgttgaaatg aagcatgctt tgcatgagat cgtagaaaaa aatacaactt
    25801 tggagatgga aaatcaacgt ctgcgggaac acctgcggga attgaaccaa gttgcacaag
    25861 ctccagctga aaatgtgaag caggaacttt ctaagtctcg tatgaatctt gaaaaaattt
    25921 atgaagaagg attccatgtc tgctacgatt tatatggatc aagaagggac aatgacgagc
    25981 cttgtgcgtt ttgtctagaa gtgatctatc gcgaaagcgg tcgatgacca ataacaagag
    26041 gagactggag cggagaaata ctgctccagt ctttttatta aggagattgt gacagatgta
    26101 taatcaaaaa agtttcaagg gaaatccggt atatggggat ttatatcttg taccgacgcc
    26161 aattggaaat ttagaagata tgagctacag aagtgttcgt atgctgcaag aatccgatct
    26221 tatcgcaagt gaggatacgc gtaatacaca aaagttattg aatcattttg aaatccatac
    26281 accccaaaaa agtttacatg aacataatta taaagaacgt gtccctcaat tgattgcttt
    26341 gttaaaagaa ggaaaaacga ttgctcaagt cagtgacgca ggaatgcctt ccatcagcga
    26401 tcctggacat gagctagttt tagcctgcat aaaagaaggg atttctgtga tctctatccc
    26461 aggacctaca gctgggatga cggctttgat tgcttcaggg ctagtgcctc aaccattttt
    26521 attttatggc ttccttccac gtaagaaaaa agaacagaaa gaaacattga aaaagctaaa
    26581 agaacagaca gttacgctga ttttttatga gtcaccttat cggatcactg ctacattgac
    26641 gaatttctta gaaatttttg gaaatcgcca agtcgttttg tgtcgggaat tgacaaaggt
    26701 ccatgaagaa taccttagag gaagtacgga agagctactc gcttatatcg aagaacatcc
    26761 agtaaagggt gaatgttgtc tgcttgtaga agggaatttg ttcgaaatgc cagtgacgga
    26821 agttgatgaa gagttgggaa cgttaaagga acaagtagaa gcaaagatcg cttcaggcga
    26881 aaaaccaaat gctgctatca aagcagtagc gctgaaaaat gggctgaaaa aacaagaagt
    26941 ctaccgtcag taccatgaat tagactgaga tagtataagt tgtctataat gaaagactaa
    27001 tgaggaaatc agctactaac ttttcaaatc tcttgaacgc gaacgaatgt tcttgtataa
    27061 tgaataagag gtgaggatga tggagctgca atttccttta cataatgata catcaagaaa
    27121 aatcattcat attgatatgg atgctttttt cgcttcggtt gaagaacgag atcatcctga
    27181 gtttcgcgga catccattag taattgccag acaccccagt gacactggtg ggcgtggagt
    27241 agtgactaca gcaaattatg aagcaagaaa attcggaatt cattcagcaa tgagtgcaca
    27301 aaaagcgtat gagctttgtc cgcaagccat ctttaagcct ggcgaccatc ataaatacag
    27361 cgatatttcc aaacaagtac gggaagtatt ctatcgttat acagatattg tggaaccagt
    27421 tagtatcgat gaagcgtatc ttgatgtgac cgaaaataaa atcaattgca agtcagccat
    27481 caaaatcgcc cgaatgatcc aagcagatat ttgggagagt gtccagctaa catgttctgc
    27541 gggagtaagt tacaataagt ttttggccaa attagcatca gactatcaaa aacctagagg
    27601 aatcacggta gttgcccctc aagacgctgt gccattttta aaagctttgc caattgaaaa
    27661 atttcatggg gtagggaaga aaactgtacc tagaatgcat gagctaggga tttatacagg
    27721 aaaagaccta tatgaatgca cagaaatgat gttgattcga aattttggaa aaatgggcta
    27781 ttctctttat cgtaaagtca gaggaatcca tgattctcca gtaaacgtta caagagagcg
    27841 aaaatctgtg ggaaaagagc atacttatgg gcagccgttg caaacggaag aagcagtgct
    27901 gactcagttg cgccaactag ctgaaaaagt agaagaagca ctgagaagag tccaaaaaca
    27961 tgggaaaaca gtagttttga aggtcaggta cacggactat tctactgtga ccaaaagagt
    28021 gacattgcca gaatatatct ataaaaaaga agcactcttt tatcaagcaa gccttatatg
    28081 ggaggaaatc ttgggagtag aaaaaggaat ccgattgtta gggattactt tgacgaatct
    28141 ggacccgatg acttatgaaa acatcgtcct tccattatgg gaaaatcaag aaatataaaa
    28201 acagctttga gagttgaaac cggaaatccg ttttctctcc caaagctgtt tttatatcaa
    28261 gtcttgcttt ttgatttgtt cataggtttc tgttgcatcc gtacattttt cccaaatcac
    28321 aacttgattg gattctaaag atttttggac gtcaatgatc cgctggttgc tacttccgcg
    28381 aaattgtagt tttaggtcac gtttgcttaa ttcaaagcgt ccgtcaacaa ggatatcgat
    28441 ttgcgaaagg agttctaatt tatcttctga atcttgtagc aattcatcaa aggtatagcc
    28501 tgtccatgac cagatatctt ttgtcgtacc gaactctttt cggatacgtc gtacgacttg
    28561 cagacaaaca tctgtattca aaaaaggttc gccaccaagt aaagttaatc cttgcacgta
    28621 atcgttgcgc aagtcagcga tgatttgttc ttctaattca ggtgtgaatg gtttgccata
    28681 tcgaaaattt tgtgcagctt tgttgaagca gccttcgcag gcaaataagc agccactaac
    28741 gtaaagactg ttgcggacac cttcaccatc gacaaaatta aacggtttgt agtcagcaat
    28801 atagttttgg ctgtagtctt ttgctaacca ttcttttggt tttggatttc tcataaaaaa
    28861 acttctttca tttaaggttc aaagatatag ctattgaaga taaaatctac tcgaccacat
    28921 aaataagaac aaactgacct aaatccattg ttcaagaaag cttgttctta ttttgataga
    28981 gggcgttcaa agatacaaag ccatcgaaag agggtggtga aaaaagactt gtcatagcct
    29041 cttattctga ataaacggtg ttcaaaagct tgcatctgca gtaataagcc caactaaacg
    29101 aaaaatatga ggaactattt ttgtttagct attcttatta cttgatgcag gacagctttt
    29161 tcacaacctc atttcatgtg tttcacacgt gaggaaattt ctttatggcg gccatgtacc
    29221 atcggacgag cttgcggatt tcctaaatag ccacaagtac gtttgactac atcacatgtt
    29281 tttggatcat gatttccaca ttctggacat tcgaaccctc gttctgtcgg tttgaaatca
    29341 ccttcgaaac cacattgata gcaatgatcg attggcgtat tcgtaccaag atacccgatt
    29401 ttgtcatatg caaaatccca tacagcttct aaagctttcg gattttgttg gagcataggg
    29461 tattcacagt agtggatgaa tccacctgaa caatattttg gataatcctt ctcaaattcc
    29521 aatttttcaa atggcgttgg gtttttacgc acatcatagt ggaaactgtt tgtataatat
    29581 tctttatctg tgatattttc gatgatgccg aatttttctg tatccaaacg gcagaaacgg
    29641 tcagttagac tttcacttgg agttgaatag acactgaaat gataaccata ttcatccccc
    29701 catgcatctg cattagcttt taattctttg acgatatcaa gtgtgaaagc ttttgcttct
    29761 gggtttgttt cccattcgcc accgaagaaa gctgaagcaa cttcatacaa tccaatgtag
    29821 cctaacgata cagtagctcg acggtttttg aataattcat tgacagaatc ggtacgtgaa
    29881 agacgtttcc cgaaagcacc atacatatat aaaattggcg catttgctgg aatggcttct
    29941 ttacaacgtt ctacacgata gagaagcgca tctttcatga tttctaagcg gtcagacagt
    30001 aactgccaga atttattctg atctcctttt gattcgatcg cgattcttgg caaattcaat
    30061 gtaacaacac ctaaattcat tcgtccgaca tttacttctt gaccattttc gtctttccac
    30121 ccttgcaaga atgaacgaca gcccataggt actttgaaac ttccagttaa ttcaacgatt
    30181 ttatcgtaat tcagaatatc aggatacatt cttttcgttg cacattccaa tgctagttgt
    30241 ttgatgtcgt aattaggatc ttcagggttt aagttcacac cttttttcaa agtgaagatc
    30301 agtttaggga agatagctgt ccgcttttca ctccctaatc cattgattcg aatctgtaaa
    30361 attgcttttt gaatttctct ttcaaaccag ttttctccta gtccaaatcc taatgaagtg
    30421 aaaggagttt gtccatttga agtaaataag gtattgattt catattccaa actttgcata
    30481 gcatcgaaga tatctttttt ggtttttgct tttgcatatt ctttttggcg ttctggcgaa
    30541 tcgatccatt cttccgcgtc ttttagatgt tttttatagt tcaattctgc aaaaggagca
    30601 agcaattcat ctgttcggtc ggcagaacag ccaccatatt gacttgacgc tacattggca
    30661 ataatctgtg agatttgtgc tgtggcggtc tggatagatt ttggcgattc gacttcggca
    30721 ttgccgattt tgaacccatt gttcagcatg cctttgaaat caatcaagca gcaattcgtc
    30781 atcggtgtat aaggatgata atccaaatca tggtaatgga tatcgccttt ttgatgcgca
    30841 tttgctatat gtggtggcaa cattttcagt ccgattgatt ttccaacgat cccggctgtc
    30901 agatctcgct gtgtattgaa aacattactg tctttatttg cgttttcgtt cacgacagtt
    30961 tggtccttgt tgattagctt ttcaatcgaa aagttgatat ctgttgcttt actgcgagca
    31021 aaatctcgtt tggtacgata attgatgtac tcttctgcta attcatattc gtttttttct
    31081 agtaatatat gttccacgat gttttgaatc tcatagattt tgacattttc agcaaaccgg
    31141 cttttgattt cttggacaac caagtctgca atcatttgaa ttttttcata agtaagtggt
    31201 gataatgagc cgtgaatttt cttttctgct ttgatcagtg cttcattgat cttttgttca
    31261 tcgaaatcaa catggcggcc atcgcgtttg atgactttca tctgggatgt ctctggctgc
    31321 tgagtaacga cagttttttc ttcataattt actttcatta tcttcaactc ctgctaaatt
    31381 gttcttgtct atcataaaac aaaatcgggg aaggagcaac taaatatcgg ctatatatag
    31441 tattcaatgt gctgtataat tggatttcta tactatatgt agtgtatatt ttattgtgaa
    31501 aaaaggtgaa aacttgtagc tggtggattt gttttttgca tatttaattt tgctgaataa
    31561 aataattgaa taaaattttt tattcagtcc aagtaaaagt gacaaaaaca taagagcaaa
    31621 ttattcgtag atatgctact atgcaaataa atagaaatga aaaatgaaag aggaggagaa
    31681 gaattggaaa agatgatcga agtgaaacat ctaagtaaaa gctacggcag caaactttct
    31741 cccgtcaaag ccttagataa tatctcattc acagtagaag aaggcgagtt tgttgggatt
    31801 atgggcccaa gtggtgcagg aaagacaacg ctgatgaata tcttgtccac gatcaatttg
    31861 cctacgatgg gcagtgtctc gatccaagga aaagaaatca caaaaatgaa aaacagtgag
    31921 ctcagtgact ttcgtcgaaa aaaactagga ttcatttttc aagagttcaa tttgatcgat
    31981 accctaaatg caaaagacaa tatcctattg cccctagcag ttgaacggat gactaaagag
    32041 gaaatggaaa agagagtaag acatgtagct caattgttga atatagaaga actgctcaaa
    32101 cggtatcccg atgaactttc tgtcggacag agacaacgaa ttgcagctgc tagagcactt
    32161 gttgttcagc cacaagtgat ttttgcagat gaaccaactg gctctttgga ctcgaagtcg
    32221 gctacagaat tgttgaacta cttgaagacc atgaatcaaa aagaaaaagc aacgatactg
    32281 ttagtcaccc atgatccata tacagcaagc tactgcgata gaattttatt tattaaagat
    32341 ggtgtgattt tttctgaagt tgtcagaaga gggacaagaa gagagttctt tgagaaagta
    32401 atagacatgc aggcgacgat tggcggagga ggaaaaatga atgctgtata aactagccgt
    32461 taaaagtttt tttcggcagt cacgaggcta tcttgtttat tttttcagtt tgactttatc
    32521 cgtaatgatc tattattcct tcagcgcaat gacctacgat caatctttag tcagaagagc
    32581 aagtcaagat acttcgatag atggtggatt gagttttggt ggatttatca ttactgttgt
    32641 tttgcttttt ttcgtattca gtgccaaccg atttttcttg aatagaaggc aaaaggaaat
    32701 aggcatctat cagctttttg gtatgaataa actgcagata tcaggtatat atgtgttaga
    32761 aatcatgata attggattgt tcgcctgtat ttctgggata ttactcggta ttattttttc
    32821 caaactcttt tcaatgattt tggttcgaat gatggatatg gatcttacta gtccattttt
    32881 tatctccatt ccatcagtag tagatacttt atttgctttt ttcatcatcc ttttagcagt
    32941 atctgtatat tcaatatgga aaatatggcg ctatccaatg attcgaagtt ttggagaaaa
    33001 agaacaagca gatagttcaa caatgcgctt caggacaagg caccgattac tcgcagtcat
    33061 cgggttacta cttttaacga gcggatattt tggagctagt cattttaggg aaatcacttc
    33121 atggatgatc agttatcatt atcttgatac attagttttc ttttttcctt ttttaatttt
    33181 tttcgtttgt gtggtcggta cttatttgtt tttctgttat acatttcggt tatttttgat
    33241 catattgagt aaatggaaga tttattacta tggtatcaat tttttgataa tgggtaacac
    33301 gcaagttcat ttgctcaaaa gttggcgtac aaattctctg attacagtca ttctcggaat
    33361 ttctttaatg acaattggcg gcacggctag tatctctact attttatcct accaggagaa
    33421 agtttcctcc ccgatggact atcagtttga tgttgagaca gagaaaaaaa tcaagccgat
    33481 tctagcagag gaagggcaga agataactaa agaaatcacc ttgcattata aagtcattgg
    33541 gagtatctat agtcaaaatc tcgaagaacc tatgtttcaa actgctaatc tgatcacgga
    33601 aacacaatat caagccttca gaaaagttaa tccagatttg ccgaacattt ctttgaaatc
    33661 acctggtcat actgttttgc ttgatcaaat gcaaactctt tttcggaata tttcattata
    33721 tggatctagt attggactgc cagataatca agaattgaag attcaacaaa tgcttccaag
    33781 tgtattaggt gacgaaacaa tggtatatct aagtcctgta ctgattgtag atgataccgt
    33841 ttttgaaaaa gcaaaaggtg tggattatca agtagtcaat gtagatgtat caggtggaaa
    33901 taatgaaaaa atagatgaac gactgagcaa tgacctgtcg ataaagtggc tcaatgctgt
    33961 ttattattct tatgatatcg taaatggaag tctacaagga gaaattagca caaagaaact
    34021 tccggatgac caaatgaagg aagcacaatt tagccaagaa agctctcgtt tgaatttctc
    34081 ttccagctat tcgaaattgc gtgcagttaa tcggcgaatc ggtatcaata tttttgtcac
    34141 actatttgtc gggatgatcg tgattattgc tactggtagt attttgacag tccggcaatt
    34201 agcagaagca gaggaagaaa aagaaaatta tcacctattg actaagctgg ggattccaca
    34261 aagaaggatc agacggatga tcttcgagca aaacgcactt accttctttc cacccatgat
    34321 attagggatc actcacgcag tatttgcaat caatgtcttc actcagtata taaaaactgc
    34381 cgattactgg ttggcttatc ttgtgagtgg cttattgatc cttatctatc ttttgcttta
    34441 tgtcattacg agtcagttat attgtcggat tatcgaggaa tgatatacgg aataagacaa
    34501 gataataaat agaactaaat tttgaatac
//
LOCUS       DAZKLM010000022        31702 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3764
            SAMEA115706913-rid22582563.denovo.022, whole genome shotgun
            sequence.
ACCESSION   DAZKLM010000022 DAZKLM010000000
VERSION     DAZKLM010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706913
            Sequence Read Archive: ERR13196647
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 31702)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31702)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287823.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287823.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,004
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,849
            CDSs (with protein)               :: 2,849
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 41 of 96
            Pseudo Genes (incomplete)         :: 52 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 15 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31702
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706913-rid22582563.denovo.022"
                     /isolate="VRE3764"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(1014..1355)
                     /locus_tag="ACG842_000962"
     CDS             complement(1014..1355)
                     /locus_tag="ACG842_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFQ0247524.1"
                     /translation="MSEERIYIPKKGDIVWIDFDPSVGKEIQKRRPGLVVSRYEFNRK
                     TMFSVICPITSTIKNLPTRYSLPEELDIKGQVIITQLKSLDFKERKLKKIENLPLQDM
                     AKIDQIIQYMF"
     gene            complement(1355..1585)
                     /locus_tag="ACG842_000963"
     CDS             complement(1355..1585)
                     /locus_tag="ACG842_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFQ0247525.1"
                     /translation="MLVTKSRLQGSSVVVTLPSDNGKKPSENQEYIVVYSDDGTITLV
                     PKIEDPFSGGEEAEYYEKDEWEDLTPEGREIL"
     gene            1872..2210
                     /locus_tag="ACG842_000964"
     CDS             1872..2210
                     /locus_tag="ACG842_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0247526.1"
                     /translation="MPDYKQKAGYSFCSDKFQLGKFTKLAGEEVSTVRIKECPLQIET
                     LVQRISTRQDFAIVEREIVAIFVEEGILHDPTHINVDKWQPLIYKFREYTTANQSLTL
                     NFRFQEFKES"
     gene            complement(2415..2977)
                     /locus_tag="ACG842_000965"
                     /pseudo
     CDS             complement(2415..2977)
                     /locus_tag="ACG842_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349929.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0018142 - obsolete protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
     gene            complement(3709..4290)
                     /locus_tag="ACG842_000966"
     CDS             complement(3709..4290)
                     /locus_tag="ACG842_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFQ0247527.1"
                     /translation="MKTKEKILEATKDLIYKKGYHETSIRDILAASDTGKGQFYYYFS
                     SKKEVCLAVIHAHMDVWQTELFEDILNAGKQPEEALEEMLDWIFADHETQITYHGCPV
                     GNLIIELSTVDEEYRLLLLELVNQWVKYTKQNILALSEEKLSDEEADRQAKALIAEIQ
                     GSILLLKVTQDLSLLRQNFQLIKERLLTSQKVL"
     gene            complement(4473..5099)
                     /locus_tag="ACG842_000967"
     CDS             complement(4473..5099)
                     /locus_tag="ACG842_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HFQ0247528.1"
                     /translation="MFNNKNTAVVLTDPQVEFLKPAGGGFGLTKDILEKYNTIENMIE
                     MVKQAKEKGYKLFISPHYFYPHDHKWQFGGAGEHMMLEQQMFARKSQYESVEEGSGAD
                     FIEELKPYLDENTIISSPHKIFGPESNDLALQLRKNGIDTVILGGMNGNLCSDSHMRE
                     LVESGFNVVVATDAIGAPGQEAMDAALTNFGFIADQVMTTEEVLEQLS"
     gene            complement(5121..6452)
                     /locus_tag="ACG842_000968"
     CDS             complement(5121..6452)
                     /locus_tag="ACG842_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016668 - oxidoreductase activity, acting
                     on a sulfur group of donors, NAD(P) as acceptor [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HFQ0247529.1"
                     /translation="MMEKYQNIVIGFGKGGKTLAKALASKGESVLVVEKSTRMYGGTC
                     INIGCIPSKSLIFNGERGIDFTEAVARKEKLTGMLRAKNYHMISDEATGTVMDGTARF
                     LSNHQIEVTNNGEKVIVEGERIFINTGSEPIILPIKGLNTSRYLIDSTQAMDQEQLPE
                     KLVIIGAGYIGLEFASMFNEYGSKVVVLDAHSEFLPREDEDIAQMILEDMTNAGIEFH
                     LGVSVDQVADQESTAAVTFTEDGQEVTIQASKVLAATGRKPSTASLGLENTDVKLTDR
                     GAIAVDEYLRTDADNIWAIGDVKGGLQFTYISLDDYRIIMDQLKGENKRTTNNRKAVP
                     YSVFITPTLSNVGLTEKQAKAQGVNYKLFKYMTSGVPKAQVLEDPKGVFKVLVDPETD
                     LILGASIYAEDSHEVINLISLAMNGKLPYTLLRDQIYSHPTMSEALNDVLK"
     gene            complement(6607..7008)
                     /locus_tag="ACG842_000969"
     CDS             complement(6607..7008)
                     /locus_tag="ACG842_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HFQ0247530.1"
                     /translation="MKKAYETAMINHGGRNGEVEAPNGSMHMKITPPGIHAEGTNPEQ
                     LFAAGYASCFNGAVQHMIKEAGMTAESTVKARVSLYNLDDGGYQIGVVLEVHIDGISE
                     EEAQKIAEDAHEFCPYSKATRGNIDVEVTIV"
     gene            complement(7094..7300)
                     /locus_tag="ACG842_000970"
     CDS             complement(7094..7300)
                     /locus_tag="ACG842_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0247531.1"
                     /translation="MVTNMVIFLTVLVYGVNFLVYMYLKINHIKDYVEKIAIYFGTNM
                     TLLFTSSIFLFFGKIVEDGILVFE"
     gene            complement(7395..8897)
                     /locus_tag="ACG842_000971"
     CDS             complement(7395..8897)
                     /locus_tag="ACG842_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HFQ0247532.1"
                     /translation="MKDMKALQNGSDIRGIALPVEGYEVNLTTDMVKKIGWGLANWIK
                     KKKKSTDPNKKWTIGIGHDSRISGPALKEALIQSLKEQGIHVIDFELATTPAMFMSTQ
                     FEQFSCDAAVMLTASHLPYYYNGIKFFTSEGGAEKEDIAFILSQQQKIKSEQTGSVEK
                     ADLLTPYAQDLVTKIKKGIGTLESKPLNGWKIIVDAGNGAGGFFAEKVLNSLGADSTG
                     SQFLEPDGRFPNHIPNPDNKEAMESIKQAVLANEADLGIIFDTDVDRSAVVSASGTVI
                     NRNNLIAVLSTIVLKEHPGTRIVTNSPTSSHLQRFIEEKGGIQVRYISGYRNVINKAL
                     ELNRLGKDTQLAIETSGHAAFKENYFLDDGAYVIAKILMLLPELKRKGITLDEQIKEL
                     KQPVEVQEIRLKLFSDEPREQGNKILEEMKRLLEEEKGMNFDPENEEGIRYDLEEPYG
                     NGWFLIRLSLHEPLLVLQIENDEFGKNKLALVKLAKLFGHFSDLESLTAE"
     gene            complement(8915..9370)
                     /gene="argR"
                     /locus_tag="ACG842_000972"
     CDS             complement(8915..9370)
                     /gene="argR"
                     /locus_tag="ACG842_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006525 - arginine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HFQ0247533.1"
                     /translation="MKKKQRQALIRQIITEQPIGTQEELLARLHEAGADVTQATISRD
                     IREMKLVKSQNENKIVRYTLFNQPSVSLNEERLRTAIRREVLRIQSVQFMVIVLTERN
                     GADVVTNWLDEVAYPEVVGTMAGVDTFIIICRSEEEAQRFAEKLEKMRE"
     gene            complement(9468..10085)
                     /locus_tag="ACG842_000973"
     CDS             complement(9468..10085)
                     /locus_tag="ACG842_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFQ0247534.1"
                     /translation="MEKIEWRKDKSYSMKKSPQILEVPKKSFFSIRGIGDPNQEDFKR
                     RVACLYAVSYAIRMSPKKNWLIPDYQMYTVYPLEGQWGLQEKYLHEPVMKKEHFSYQL
                     MIRQPDFVTEAIAQEAIQRSSKLPEDLKEQVVFKKMEEGLCAQILHVGPYAEEPATFE
                     KLEAFIAKEGYERASKEHKEIYISDPRKSAPEKMKTILRVKIEKG"
     gene            10306..11679
                     /locus_tag="ACG842_000974"
     CDS             10306..11679
                     /locus_tag="ACG842_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFQ0247535.1"
                     /translation="MTKQKQLLLVPAVIATGMMSFAGVLIETAMNVTFPTLIRQFDLT
                     TAQVQWVTTIYLLMISIIVPLSTYLNRNFSLKKLFLASNLLFLAGIMIDFFSPTFMVL
                     LFGRLLQGAATGIALPLMFHIILTFAPLNRRGAMMGVGTLTTAIAPAIGPTYGGIMTS
                     HFTWNHIFLFLVPVLFLSLIIGWFAIPEMAVQKSGYLDWISVLGICLLFSGSLTFLST
                     LGTVSSWIALIIGGIGWFLFYYRSKKVEHPLIRLSILSNKTFRLFLIGFLVYQFLLLG
                     VSFVLPNFLQIVQGSSPFVAGLAMLPGAAVGALLSPFSGKMLDQYGPKKPIFAGLILS
                     LIGWLALFLLIGHASVGVLIACHVFYMIGIGLSYSNMMTTGMNALNEELQGDGNAMFN
                     TLQQFSGAVATSLVAVIINYVQHHTSHNYEVSTTLGSKAALGVLLLLLLVSFARFAYY
                     LFFAKKA"
     gene            11754..12866
                     /locus_tag="ACG842_000975"
     CDS             11754..12866
                     /locus_tag="ACG842_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFQ0247536.1"
                     /translation="MEKIAIIGGGIIGMTLASYLDTSKFNVALFDEGTGQATKASAGI
                     ISPWLSKRRNKQWYRLAKDGAAFFPKLIKDFQLDQTIYAQSGTLLLRPEKDLAELAVL
                     AEKRKEEAPEIGNIEWLSPEQTSQALPLLKPAVSLRISGGGRLDGKAYLEHLGQRLAQ
                     KQMEQIQAKVTLHKKSDSWLIKGPFGEYTADRVILTPGPALKPLLQELGYAADIRPQK
                     GQLLAFQTSYKMSQDWPVAMLDGEADFIPFMDGKILLGATHENDQGWDLAPTSEAYKG
                     LTKSTEQFLTDPDQIKNWPYEFRVGTRAYTSDFSPFFGVLPDDPSLLAASGLGSSGLT
                     TGPYIGYLLANFLNTGHWPTEDYQKDISTYITKNPS"
     gene            complement(12938..14575)
                     /locus_tag="ACG842_000976"
     CDS             complement(12938..14575)
                     /locus_tag="ACG842_000976"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HFQ0247537.1"
                     /translation="MSTKESLELNEITSGNPLLSSIRSIVETTFYGNNVHEVFDRKTA
                     YQLAKGSPGTIITDLTISHAEELDLPADVRTLVFNDGSIVGRTASARRIFEDLDKEQS
                     KYEKILREAVYQSRKRQFYHTKVIVGLSEEFSVQSHLLIPEGFENNLYSYLLNFQPAN
                     SKILETYQKSVSFDEGDLYIFCDPEWTHETFPNGLILFDALHNTAAVLGLRYFGELKK
                     ATLTLAWALGNRNGFIACHGGMKQYASNDQTYTMAVFGLSGSGKSTITLAKHTEGHSV
                     SILHDDAFVISRKNGFATALEPSYFDKTQDYPSDDPAISSFLTCQNVGVTCTSKGEKV
                     LVTGDIRNGNGRTVKSRYATPDRVDHLSEKIDAVFWIMKDDSLPPIIKINDPVLAASF
                     GATLATKRSDAENITTKRQLDELVIEPFANPFRVYPLREDYQAFRELFLKQQTACYVL
                     NTGYFDDKKIKPETTLSIVERIVQQTAEFKSFGPFEDIHYLPIPDFPVSFEDTSYKNL
                     VKQRLDIRLRFIEAQIGKDLLPDEAKESLENLIQKLN"
     gene            complement(14743..15351)
                     /locus_tag="ACG842_000977"
     CDS             complement(14743..15351)
                     /locus_tag="ACG842_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFQ0247538.1"
                     /translation="MNSFKKIVLGTTFAAGATAMFVGTTNAHADEVYTVKSGDSLSKI
                     SQKFAGDNSMIDAIAEKNSIANINRIYVGEQLTIPTSNDSSATTENKTTENTASTTET
                     ATQEHTYVAPVETVEVAPAAPAAATAPTSSSAKEWIAQKESGGSYTATNGRYIGRYQL
                     DASYLNGDYSAANQERVAEQYVTSRYGSWDAAKTFWLANGWY"
     gene            complement(15735..16886)
                     /gene="queG"
                     /locus_tag="ACG842_000978"
     CDS             complement(15735..16886)
                     /gene="queG"
                     /locus_tag="ACG842_000978"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HFQ0247539.1"
                     /translation="MTLKEEIIQESKRLGIDKIGFTTAEPFDSLKESLEEQKAAGHTS
                     GFEHPNIDERLYPEMTFDQPKSIISIALAYPTRIHEEVPRDEKRGQFARASWGIDYHD
                     ILRDRLNRLIEFIQTRAEKWQKEEEWRLAPQVDTGELIDVAVAQRAGLGFIGRNGLLI
                     TEEFGSFVYLGEIVTNIVFEPDKPGEFGCGDCMRCVTACPTKALLGDGRMNAQRCLSY
                     QTQTKGMMPEEYRKKMRNVIYGCDICQLVCPYNQGKDFHFHEEMEPKVDEIYPKLKPM
                     LSMSNKDFKQQFGHLAGSWRGKKPLQRNALIALANLGDRTALPEISECLTDVRPVIRG
                     TAAWAIGRLGTKEPEKWLDVLDEILAKETEEVVINELEHAQKLLRRKLK"
     gene            complement(16905..17657)
                     /locus_tag="ACG842_000979"
     CDS             complement(16905..17657)
                     /locus_tag="ACG842_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0247540.1"
                     /translation="MNFTRKDKYTIKEEVFRLISQQENTDKDQDLQFLEAELAKLFIA
                     NQNETTEPDEELYIGKWLFDEFQPLDENTGIGYFVFLSPVGFTPGVLVKQNDEITVKA
                     WPALFSVIKAEGVIKKIQSAYYKSQFIANSKLMNMFYNRLVEIARPGKIPQTFTYTQQ
                     KNLYEEVLYSRDLSEFSPWEEVRLHELLSTKPVSLNFFNALDNKRKLISTNYWEVFAA
                     RKKWMEEKKIADQQIQFVQLKKEKLYYGLYSR"
     gene            complement(17798..18748)
                     /locus_tag="ACG842_000980"
     CDS             complement(17798..18748)
                     /locus_tag="ACG842_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HFQ0247541.1"
                     /translation="MKTKKSLFLILAVSFLVLAGCGKQASDQADEGSKEKLSVVATNS
                     ILADMAKEVGTDQIDIHSIVPVGTDPHEYEVLPEDIKKASDADVILYNGLNLETGNSW
                     FDNLMETAKKEEGKDYFAVSKNVEPLYLTSGEEHTKADPHAWLDLSNGIKYVEEIARI
                     FSEKDAENATLYKKNAEAYVEKLKELDTQAKESFASIEENKKLLVTSEGAFKYFSRAY
                     DLPAAYIWEINTESQGTPDQMKAIIDQIRAKEVPVLFVETSVDSRSMERVAKETGLKI
                     YDKLFTDSIAKEGEQGDSYYQMMKWNIETIHEGLSQTKES"
     gene            complement(18749..19588)
                     /locus_tag="ACG842_000981"
     CDS             complement(18749..19588)
                     /locus_tag="ACG842_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HFQ0247542.1"
                     /translation="MIQSFIDGLMNYQFLQYALVTSMLVGLASGVIGSFIILRGMSLM
                     GDAISHAVLPGVAISYMFGFSYIFGATAFGMLTAAVIGFVTQRSRLKNDTAIGIVFSS
                     FFALGIILISYAQSATDLYHILFGNVLAVRESDLLLTALVSGIVLIFVFFFYKELKIT
                     SFDPTMAKAYGLNTSLIHYLLMFFLTLVAVVSLQTVGTILVIAMLITPAATAYLLTNH
                     LLKMIITAAGIGMLSAVVGVFFSYSYNWPSGATIVLACTAFFILAFLFSPTKGILFQK
                     GSR"
     gene            complement(19585..20331)
                     /locus_tag="ACG842_000982"
     CDS             complement(19585..20331)
                     /locus_tag="ACG842_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HFQ0247543.1"
                     /translation="MERTAIVIQDLSAAYQGKTVLKTINLTINPQKITGIIGPNGAGK
                     STFMKSLLELIPATTGKVTFSGQSVNSVRKKIAYVEQRSELDLSFPIDVLGVVLLGTY
                     PSLRIGQRPGKKEKERARQALKKVGMEEYAKRQISELSGGQLQRVFIARALAQGAEWI
                     FLDEPFVGIDALSERKIFDILQELKNSGKTILIVHHDLHKVEEYFDEVILLNKQLIAS
                     GPVQEVFTSKNLQLAYGEIIRHLVKGGEKK"
     gene            20569..21939
                     /locus_tag="ACG842_000983"
     CDS             20569..21939
                     /locus_tag="ACG842_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFQ0247544.1"
                     /translation="MEEEKLTRSLSARHIQMIALGGTIGVGLFMGASSTIRWTGPSVM
                     GAYAIAGFFLYLIMRALGEMLYVDPSTGSFANYASEYIHPVAGYLTAWSNIFQYIVVG
                     ISEVIAVGSYMNYWWPDLPAIIPGIIVVLFLMLANLISVKAFGELEFWFSIIKVITII
                     LMIIAGLGVILFGFGNHGQAVGISNLWKNGGFFTGGVKGFFFALSIVVASYQGIELIG
                     MTAGEAENPKKTIIEAVQSTIGRILIFYIGAIFIIVSIYPWNQLSEIGSPFVQTFSKV
                     GITFAAGLINFVVITAALSGCNSGIFSASRMVYTLAINGKMSKKFLKLSRHGAPFYPV
                     VAISGGILIGLILNYLLPMFINQSENIFVFVYSSSILPGMVPWVVILISEIRFKKKHA
                     DKMHDHPFKMPLSPYTNYVTLAFLAIVLIFMFINPETRISLIIGLVFLLYMTIHYFVR
                     EKRTGK"
     gene            complement(21994..22890)
                     /locus_tag="ACG842_000984"
     CDS             complement(21994..22890)
                     /locus_tag="ACG842_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFQ0247545.1"
                     /translation="MYDENEWGENVRMTRRRQQKQAARKRMIMIGAAVAVFLIIAGGV
                     AGLYFHGKNSSEAKSTTITETTTKKTETKQTSESSTKTKETQTSEEKTANGLPEQLQA
                     IVNDYSKKIEEKTPALIEEYQTEIQGNQEGIAGLSAVANQKARELQAISDEGIRKLRA
                     AYQAAENKDGVDLDTLINQLSANYTNHVAKISDIYLQTSASLQAESTSTQDTTSSDSE
                     TAESVEASEMQARTTQDTTDSSETNTNSATSQAATTVVLEGEGPNQIAERTGVPVETI
                     LSLNGMSMENYFLTPGETLKLN"
     gene            complement(23004..23174)
                     /locus_tag="ACG842_000985"
     CDS             complement(23004..23174)
                     /locus_tag="ACG842_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0247546.1"
                     /translation="MTNDWLIQQLDELSEKSEYQDRALLYEIKKLMAEIDERTMQLQG
                     EIDGQSWNHLNW"
     gene            complement(23171..23848)
                     /locus_tag="ACG842_000986"
     CDS             complement(23171..23848)
                     /locus_tag="ACG842_000986"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008753 - NADPH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH:ubiquinone reductase
                     (non-electrogenic) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HFQ0247547.1"
                     /translation="MRTLIIISHPTIEESLNQQFFKEASAGLPATWHHLESSYPDGKI
                     DIETELQLLAQHDRIIFQFPFYWYSSPAHLKIWQDNVLERAAHVLKGKELGIVLTTGV
                     AEKEYQAGGKEEYTISEFLRPYQRIANKFHMTYLPPFVLAQFMYLSQEKRWEKLIAYQ
                     QYLSLEGKPSLTQRIDWFIQRVQENQKMQEEESEKQAYIVEALTEAKEQIEDLTFTLQ
                     EMKGTSL"
     gene            complement(23939..24844)
                     /locus_tag="ACG842_000987"
     CDS             complement(23939..24844)
                     /locus_tag="ACG842_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family ligand binding domain-containing
                     protein"
                     /protein_id="HFQ0247548.1"
                     /translation="MEQSIFLKKEHQQVEKELYFDFCGFSKTMPFHSFGPAIRNDYIL
                     HVVMEGKGSYHVKNQQFQLKKGDLFLIRPGDSTFYLSDGEDPWMYCWLSFGGSAAEEI
                     IRQSLFREDQYTMVSSDIQKYIDIILECMKDTEETLENELQLTALTYRFLSVLLKDGG
                     KVSGGVQKVHSSLAVETVKYIEAHYAEKLSVEEIAQHLAVNRSHLSRVFKNHMGTSIK
                     EYLIGVRINRGAFLLSLTDESVESIAYQVGFNSLVVFSRMFKKSTGETATNYRKRMKN
                     ENFKNIALDTLKKQLEKQTAVSWTT"
     gene            25019..26473
                     /locus_tag="ACG842_000988"
     CDS             25019..26473
                     /locus_tag="ACG842_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015486 -
                     glycoside-pentoside-hexuronide:cation symporter activity
                     [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside-pentoside-hexuronide (GPH):cation
                     symporter"
                     /protein_id="HFQ0247549.1"
                     /translation="MKSKMIQRVSYALGAFGHDTFYVTLSTYFMVFVTSQMFAGTDKA
                     TNAKMIATVTSLVVGIRLVEIIFDPLIGGIIDNTRTKYGKFKPWLVIGGVVSSVLLVA
                     IFTNFFGLATHNTTLFIILFAIVFILLDSFYSFKDIAFWSMIPALSTETKEREKFATF
                     ARFGSSLGANGTTLVVVPIVTFFTYLVTHKHIEGASGWFWFGVIAAVISGGTAIITAL
                     GTKEVDTAIRQQNEKAGIKDVFKAVAQNDQLMWLALAYIAYAIANAATTAVLFYFFKY
                     VLGQPTEFWIVGLVAALLGLVAVPLFPMLTRVISRRYVYLGGIAMMISSYFFFTIAGS
                     SLPIVIIGLVLFYFPQQLIFLSALMTITDSVEYGQWKNGIRNEAVTLSIRPLLDKIAG
                     ALSNGIVGFVAIASGMTGNATAADITAHGVTTFKAYAFYAPAALMIFSAIIFAWKITL
                     TEKKHAQIVEKLENKLAPADTMEDELHDAVDPSH"
     gene            26489..27520
                     /locus_tag="ACG842_000989"
     CDS             26489..27520
                     /locus_tag="ACG842_000989"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HFQ0247550.1"
                     /translation="MKITEKDFGQGYHLITLENKNKLALSISDLGARIVSLKSNDREL
                     VLGFDTAEEYIEKDPYIGASIGRTAGRIENGRFSLNGKTYQLATDPKTGHNLHGGAPG
                     FELKKWSYVILNGENEASVIFTTTSPDGEHGFPGTMDVEIRYTLTKDNIWRVTSRGTS
                     DQDTLFNPTNHVYFNLTGDASQSIDQHELWLNSEAYAPLRTDSIPIGVKENAAGSAFD
                     FQIPKKLASVFASDLDQKNLVDGIDHPFFLKETGLGKEAARLTSPDKRIQVDIATDAS
                     SVVIFTANFGTETPEMRNRKLAHHGGITFETQTAPGAERFSAFGSIHLKAGSVFETVT
                     EFKIKTRKE"
     gene            27527..28714
                     /locus_tag="ACG842_000990"
     CDS             27527..28714
                     /locus_tag="ACG842_000990"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004335 - galactokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046835 - carbohydrate phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HFQ0247551.1"
                     /translation="MEIKRSLNTKFAETFGPKKTTQYFSPGRINLIGEHTDYNGGHVF
                     PASITYGTQGVAAPRDDNKILVYSTNFDDIGVISFTLDELEYDKKANWANYVKGMVLK
                     LKEAGHSIDHGFELLVEGTIPNGAGLSSSASLELLVGVVLEDLFDLKTDRLELVQTGK
                     KVENEFIGVNSGIMDQFAIGFGELDHAILLDTNTLKYEMVPVKLDGYAIVIMNTNKRR
                     ELADSKYNERRSECEEALARLQKTLPISSLGDLDEEEFESTIDQIGDDTLIRRARHAV
                     SENQRTLKAKAELEAGNLEAFGQLLNDSHHSLRYDYEVTGIELDTLVDAAQKQEGVLG
                     ARMTGAGFGGCAIALVKEENIPAFKNNVYDEYMKVVGYAPEFYVAHIGNGTTKIDPEA
                     VHV"
     gene            28870..30252
                     /locus_tag="ACG842_000991"
     CDS             28870..30252
                     /locus_tag="ACG842_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HFQ0247552.1"
                     /translation="MWANQNPVFVYEKEPSRDILCIDCKSFYASVECVEKDLDPLTTK
                     LVVMSYPSDSTETRGSGLILASSPAAKKAYGITNISRARDLPFPYPSDLYIVAPRMAY
                     YMEKNREINSIYKKYADEANHHVYSVDESFLDVTNSMALFGKTNAYDMAKLIHKDVFE
                     QTGIYTTIGIGDNPLLAKLALDNGSKDGKQMFAEWRYEDVPEKLWKISDITDFWGIGK
                     RTAKRLKQMGIHSVYDLAHADYYHLKSKLGVIGTQLYAHAWGIDRSFLGQEYTPKSKS
                     IGNSQILDRDYTRREEIEIVIKEMADQVGTRLRRSAAKTQVVSLWVGYSLGYLDPNGK
                     TGFHQQMKIEATNASRDLADALLTIFHQHYHYQDIRRVGVNCSDLVYATGLQLNLFED
                     PEKQINEAKVDFVVDTIRKKYGFQSIIHAHSLLQGGRAIARSSLVGGHAGGMSGLEGG
                     KDETDSKKIH"
     gene            30289..30609
                     /locus_tag="ACG842_000992"
     CDS             30289..30609
                     /locus_tag="ACG842_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0247553.1"
                     /translation="MGDCFCDGRTSQRYQKNEEEARKQPLPKQQMTLQEIDSILTEAF
                     LSRKAVIIQLNLKDEFGRLLDEVEGHFIGEAYEDYFMIDHQPILWEDVRYIELKETKK
                     WFVT"
     gene            complement(30809..31477)
                     /locus_tag="ACG842_000993"
     CDS             complement(30809..31477)
                     /locus_tag="ACG842_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HFQ0247554.1"
                     /translation="MIFSITMKLVFGLIGLLLVVRLLGKKSMSEITPFDLVYTLVLGG
                     ILEESTYDDNVHVGHVLFAIALWAVMIYGIERIVQKNEKVNRWVKGEPSVLIKDGVIN
                     MTELTNNHIEMEQLRAILRQQECFFLENAKHVILENAGQMSVLKKSDEDKALSILLVD
                     EGQIQHKVLQSNQLTEAWLMENLKKEGYADVKQLIYVEWSEEKGLYILNKEDVMHESY
                     RIDG"
BASE COUNT         8979 a         6422 c         5714 g        10587 t
ORIGIN      
        1 ccaacagcaa aaaatacaat gaaaaacaaa cttggaacta atgtatcatt aataaatgtt
       61 gaaaaattca gcaactcatt actgtatgat tttagcagcc caaccaacat ataccctata
      121 aaagctaata agatggttag gaatccttta gaatttttac tcattcatag acacttcctt
      181 aaataacttt tattatagaa ggtattgtaa ataataatat tgacaaaaag tgactcaaaa
      241 taaaaaaaac cttacattat actggaatga actgtattga tagtgtacct aaaagctcta
      301 ctgacataag atcgtgtaat cggtaaagta attttattaa ttatacggga tgtaatcaat
      361 tagagaataa actaatcata aaaggtactt ttacattcat aattgtcgct ttgagattct
      421 actttggttc actatctgac ccaattgcac attttcgatc attacaagta ttggtgtcgt
      481 tagaacaact aaataaaaac tagcggtaga tttttcagaa tctatgctaa tttctgtatg
      541 gatgttccag tgatggcaaa tttctctttt cattgttttt cttgtcgcat gatatgcctt
      601 tttgtgttat tcagttaaac tatagaaatt ctttcctaga cagatcttga tcccaaattt
      661 tacgtttttg agcatcgata tagtttaaaa tgacattact catatctaaa gagtttagat
      721 tttttaggac taaaactgtc agttgttctt tttcttcttt ctgttaaagt attgtcgcga
      781 cttttcgtat acgttaagat atgtcgtgaa aaaacaagtt ttgtgacaca aaaaaacgaa
      841 tgacttgagg aaatcacctc aatcattcgt ttaaaatgct atatattctt tagtttaaga
      901 tacggaaatt aatgccatcg accacactag tagaatgaac gacatttact tggccttttt
      961 ttaatttcgt tatgtttagc atataaaaat attatagagt ttacttacaa tgcttaaaac
     1021 atatactgta taatctgatc aattttagcc atgtcttgca atggtaagtt ttctattttt
     1081 ttgagttttc gttccttaaa atcaagagat tttaattgtg taataattac ttgtcctttt
     1141 atatctagct cttcaggtaa tgagtaacgt gtcggtaaat tctttatagt ggaagtaatt
     1201 ggacatatga ccgaaaacat tgttttacgg ttaaattcat aacgagagac gactagtcct
     1261 ggtcgtcttt tttgaatttc ttttcctacg gagggatcaa aatcaatcca tacaatatca
     1321 ccttttttag gaatataaat tctttcttct gacactataa aatttctctc ccttcaggag
     1381 ttaaatcttc ccattcgtct ttctcataat actcggcctc ttccccacca ctaaatggat
     1441 cttcaatttt tggaactaac gtaattgttc catcatcaga atacacaacg atatattctt
     1501 ggttttctga aggtttcttt ccgttatcag aaggtaaagt taccactaca gaacttcctt
     1561 gtaatctaga ttttgtaaca agcattatca tcactccctt aataaaagta tactatataa
     1621 cactaagtaa cgcaaaattc ccctttgtaa actatctaga aaattgtttt gtttaaagta
     1681 tatcttattg gatataaata gaaataaatt gtagccagtt cattttttct ttaactaaaa
     1741 aaataatcca agaaacattg aacctatgga tccaaaagct acccaaatac tatagctaac
     1801 cccccgctac cttcaatttt gcagataaaa cgctttggga gaaaatttca aagacaacag
     1861 gccatcctat catgcccgat tataaacaaa aagctggcta ttccttttgc tctgacaaat
     1921 ttcaattggg aaagttcacg aaactagcag gtgaagaagt ttcaactgtt cggatcaaag
     1981 aatgcccgct tcaaatagaa acattggttc aacgaatttc aactagacaa gactttgcta
     2041 ttgtagaacg tgagatcgta gctatttttg tagaagaagg gattttacat gatccaactc
     2101 atatcaatgt ggataaatgg caacctctta tctataaatt cagagaatat acaactgcca
     2161 accagtcgtt aactttgaat tttagattcc aagaattcaa agagtcataa tgataaacat
     2221 cgttaggtat aaataaaaaa gactcgcagc acgagtaaaa aacaaataga agctatacat
     2281 tagaaaggtc gactgtgacc ctatagatgc agtatatgca gcttatgagc atttcttctg
     2341 ttctctttgt ttttgggatc tgtgctgaag tctttttcgt tttttgccgc tctttctttg
     2401 aaaaatcgat tatgttatac cttctccatt actaattcag gcatctgctg agtaatgatt
     2461 tttcgcgcaa aagaaatatc tgttacccaa ggttcgattt gttcttcagt caagacaagc
     2521 ggcattcggt catggattgg tgcgactgtg cggttaggtt cagtagtcat tatgattgat
     2581 tctgccattt tttcatcctg atggctacga taaaaaccgc caatatacac tattttttcc
     2641 ttatttgaaa acataaattt gtctttttct tcattccact cattgggaaa acacaacgat
     2701 tttgcaaaaa tggttgatga aaggtctttt tttcttctac tgtctctgca cgggcattaa
     2761 tcatcaattg tcctttttta aatccggtaa atccccactt catgatttcc ggagtgagga
     2821 tgttttcttc ctcttttctt aacgtcacaa caagatttga cggaaaaact tctccagctg
     2881 ctaattctgc tgcctgcttc ggttgtttcg tctgggcttc tcgataataa ttctgaagga
     2941 gctctgactc tagatcaaaa aaataacgac cacacatcct aatccctccc aatctttttt
     3001 tctatcatac actgcaacaa gaagttcgca ccaacaaaac gctttcaatt tttgtagaac
     3061 taaaaaagtt gtgacaagtg acaaactctc cagtgaatgg tattctcctg ccaactcctc
     3121 ggtcacaagt cacaatattc aacaaacatg agaagctgct ttttgaaatt gttccttgtg
     3181 actcggagtg aaacggcagg cttacgacct ctgggtaaac ggtgttcaaa agccagtttc
     3241 tcggtattaa gccaaattta tgcaaaaatg tagaaagcta ttttcacaaa atttttctta
     3301 atactcgaaa ctaagcgact ttttcacaac ctcttcctta aacggtgttc aaaagcttgc
     3361 atctgcggta ataagcccaa ccaaacgaaa aatatgagca gctattttta tttggctgtt
     3421 cttattactt gatgcagggc agctttttca caacctctcc actaaacggt gttcaatcag
     3481 ctaaccttcg gtattagctc aaaaaacggc aaaatatgag gaactatttt gccgtttgtt
     3541 tctctaatac tcaggtcaaa gcgctgattt cacaacctct aatacacggt gttccagaag
     3601 tagcttcttc gaaaaataag ccgaaatttc acaaaaattt gagaagcaat tttcgtaaat
     3661 tccttcttat ttcttgaagc tgaccacttc tgtcacaacc tctaattatt aaagaacctt
     3721 ttgcgaagtt aacaatcttt ctttgatcaa ttgaaaattc tgcctaagta gtgatagatc
     3781 ctgtgtgact ttcaacaata agatacttcc ctggatttca gcaatcaacg ctttggcttg
     3841 acggtctgct tcttcatcgg acagtttttc ttcagaaagt gccaggatat tttgtttcgt
     3901 atacttgacc cattgattga ctaattctag tagcaacaga cggtattctt catccactgt
     3961 actcaactca ataatcaaat tacctacagg acagccatga taagtgatct gtgtctcatg
     4021 atcagcgaaa atccagtcca acatttcttc taatgcttct tctggctgtt ttccagcatt
     4081 taagatgtct tcgaacaatt ctgtctgcca tacgtccata tgcgcatgga tcaccgctag
     4141 acaaacttct ttttttgaag aaaagtaata ataaaattga ccttttcctg tatcagaagc
     4201 tgctagaata tctcggattg acgtttcatg atagcctttt ttataaataa gatctttggt
     4261 cgcttccaag atcttttcct ttgttttcaa tgatattctc ctttttcatt tctcatagtc
     4321 ctatattata cagtaaattc atcatttttt cttcttaatc gtttaaaaaa gactgtgcca
     4381 aattatctga cacagccctc actataaaac tgttctcata tagctagatg ttcattgatt
     4441 caagaactgc gaggacaaaa gcaattccta tcttatgata gttgctctaa tacttcctct
     4501 gttgtcatca cttgatctgc aataaagcca aagttcgtca acgctgcatc cattgcttct
     4561 tggccaggag caccaattgc atctgtcgct accacaacat tgaatccact ctctaccaat
     4621 tcacgcatat gggaatcgct acacaagttt ccgttcattc caccaagaat tactgtgtcg
     4681 attccatttt ttctcaattg gagcgccaaa tcattgcttt ctggtccaaa gattttatga
     4741 ggagagctga tgatcgtatt ttcatccaaa taaggtttca attcttcgat aaaatcggct
     4801 cctgaacctt cttccactga ttcatattga ctttttctag caaacatctg ctgctcaagc
     4861 atcatatgtt ctcctgcacc gccaaattgc catttatggt catgtgggta aaaataatga
     4921 ggagaaataa agagtttgta tcctttttct tttgcttgtt ttaccatttc aatcatattt
     4981 tcgatcgtat tgtatttttc taagatatct tttgtcaaac caaagccacc gcctgctggt
     5041 ttcaagaact cgacttgcgg gtcagtgaga actactgctg tatttttatt attgaacatg
     5101 gtggattctc cattcgttat ttattttaat acatcattta gagcttcact cattgtcgga
     5161 tgagaataga tttgatctct caataacgta taaggaagtt ttccattcat agctagcgaa
     5221 atcaagttga tgacttcatg agaatcttcc gcatagatac ttgcccctag aatcaagtca
     5281 gtttcagggt cgactaaaac tttgaataca cctttaggat cttctaatac ttgtgcttta
     5341 gggacaccgc ttgtcatata cttgaataat ttgtaattga cgccttgtgc tttggcttgt
     5401 ttttctgtta aacctacatt ggataaagtc ggagtgataa atacactgta aggcacagct
     5461 ttccggttat tcgttgttcg tttattctcg cccttcaact gatccataat gattcgataa
     5521 tcatctagag agatataagt gaactgcaat ccgcctttga cgtctccaat cgcccaaatg
     5581 ttgtctgcat ctgtacgaag gtattcatcc acagcgattg ctccgcgatc tgttagttta
     5641 acatctgtat tttctaatcc aagtgatgct gtacttggtt ttcttccagt tgcagccaaa
     5701 acttttgacg cttggattgt tacttcttgg ccatcttctg taaaggtgac ggcagctgtt
     5761 gattcttggt ctgctacttg atctacagat acacctagat ggaattcgat tcccgcattt
     5821 gtcatgtctt ctaaaatcat ttgcgcgata tcttcatctt cacgtggaag aaattcagaa
     5881 tgggcatcaa gaacgacaac tttagatccg tattcattga acattgatgc aaattccaag
     5941 ccaatatatc cagccccgat gatcactaat ttttccggta attgttcctg atccatcgct
     6001 tgcgtagaat caatcaagta gcgacttgta ttcaatcctt tgattggtaa aatgattggt
     6061 tcagaaccag tattgatgaa aatccgttcg ccttcaacaa tcactttttc accattattc
     6121 gtcacttcga tttgatggtt agaaagaaaa cgtgctgtac cgtccatcac tgtcccagtt
     6181 gcctcatcgc tgatcatatg ataatttttg gcgcgaagca tgcccgtcaa tttttctttc
     6241 cgagcaacag cctctgtaaa gtcgattcct ctttctccat tgaaaatcaa tgatttagaa
     6301 ggaatacagc cgatgttgat acatgttccg ccatacatac gtgttgactt ttcaacgact
     6361 aaaaccgatt caccttttga agctaacgcc ttcgctaaag tcttaccgcc ctttccaaaa
     6421 ccgatcacaa tattttgata tttttccatc atttttccct cgcttctttt ttacctaccg
     6481 attggtacaa aaatatgata accgataatt gtctattctc caaatcatct gctttatttg
     6541 tcaaaaaaaa ccagacaaaa agcggtcctt tcacgttttt agtctggttc gatttccttt
     6601 attcttctaa acaatcgtta cttctacatc gatatttcct ctagttgctt tggagtaagg
     6661 gcagaattca tgcgcatctt ctgcaatttt ttgggcttct tcttcgctga tgccatcgat
     6721 atgcacttct aatactacac cgatctgata gccgccatca tctaaattat aaagagagac
     6781 acgtgctttt actgtggact ctgctgtcat tcctgcttct ttgatcatat gttgtaccgc
     6841 cccattgaaa caagaagcat aacctgctgc aaataattgt tctggatttg ttccttctgc
     6901 atggattcct ggaggagtga ttttcatatg catcgagcca tttggtgcct cgacttctcc
     6961 attgcgcccg ccatggttga tcattgctgt ttcgtatgct tttttcattg tactgctcct
     7021 ttcgattgac catttgctac ttggctataa ttatagtgta tgacaagtaa ggcgctttct
     7081 acaaaaattc tgcttattcg aataccagaa tgccgtcttc tactattttt ccaaaaaaca
     7141 aaaaaatact actcgtgaac aacagcgtca tatttgttcc aaaataaata gcaatctttt
     7201 ctacataatc ttttatatga ttgattttaa gatacatata taccagaaag ttgaccccat
     7261 aaaccaatac tgttaaaaaa ataaccatat tcgttaccat tttttcctcc ttttgctggt
     7321 ctccctacca gcactcgatt taaccatacc actttttttg ccgtccaagc aactagcctt
     7381 tcaaaaagac tggtttattc tgctgttaat gattcaagat cagaaaaatg tccgaataat
     7441 tttgctaatt ttactaacgc caacttgttc tttccgaatt catcattttc tatctgtaat
     7501 actagtaacg gctcatgtaa gcttaaacgg atcaaaaacc agccgtttcc gtatggctct
     7561 tctagatcat atcgaatacc ttcttcattt tccgggtcga agttcatccc tttttcttct
     7621 tccaacagtc gcttcatttc ttctaatatc ttgtttcctt gttcgcgtgg ttcatcgctg
     7681 aacaatttta atcggatttc ttgtacttct acgggctgtt tcaactcttt gatttgttca
     7741 tctaatgtga taccttttct ttttaattct ggcaaaagca tcaggatctt tgcgatgaca
     7801 tatgcaccat catccagaaa atagttttcc ttgaatgcag cgtgtccgct tgtttcgatg
     7861 gctagctgtg tatctttccc aagacggtta agttctaatg ctttattgat tacatttcga
     7921 tagccggaaa tataacgaac ttggatgcct cctttttctt caatgaatct ttgtagatga
     7981 gaagaagtag gggaattcgt cacgattctt gttcctggat gctcttttaa tacgattgta
     8041 cttaatactg cgatcaaatt atttcgattg atcactgttc cagaagcact gacaactgca
     8101 gaacgatcca catctgtatc gaaaatgatc ccaagatctg cttcatttgc taaaacagcc
     8161 tgcttgatac tttccatcgc ttctttatta tcaggatttg ggatatgatt aggaaatcgg
     8221 ccatctggtt ccaaaaattg ggaacctgtt gaatccgcac ctaagctatt caatactttt
     8281 tcagcaaaga atccaccagc accatttccg gcatcaacaa tgattttcca accatttagt
     8341 ggtttgcttt ctagtgttcc aatccccttt ttaatttttg tgactaaatc ttgcgcataa
     8401 ggtgtcaaaa ggtctgcttt ttctacgctt ccagtttgtt cgctttttat tttttgctgc
     8461 tgtgatagga taaaagcaat atcttctttt tctgctcctc cttctgaggt aaagaatttt
     8521 attccattat aataataggg aagatggcta gcagtcagca taaccgcagc atcgcacgag
     8581 aactgttcaa attgagtgga cataaacata gcaggcgtag tagctaattc gaaatcgatg
     8641 acatgtatcc cttgttcttt cagactttgg atcaacgcct ctttcagtgc tggtccgctt
     8701 atcctactgt catgaccaat cccaatcgtc cattttttat ttggatcggt tgatttcttc
     8761 tttttcttta tccagtttgc tagtccccag ccgatctttt ttaccatgtc tgtcgtcaag
     8821 tttacttcat atccttcaac tggtaatgcg attcctcgaa tatccgatcc attttgcagc
     8881 gctttcatat ctttcatata aaagtcctcc tcatttattc cctcattttt tccagctttt
     8941 ctgcaaatcg ctgtgcttct tcttctgagc ggcaaataat aatgaatgta tctacaccgg
     9001 ccatcgttcc gacaacttca gggtaagcca cttcatctag ccaattcgtt accacatcgg
     9061 caccatttcg ttcagtcaat acgataacca tgaactgaac agactggata cgcaaaacct
     9121 cgcgtctaat tgctgtacgc aaacgttctt catttaaaga aacagatggc tggttaaata
     9181 atgtatagcg tactattttg ttttcatttt gactttttac taacttcatt tctcgaatgt
     9241 ctcgagaaat cgttgcttga gtcacatcgg cgcctgcttc atgcagccgc gcaagcagct
     9301 cttcctgtgt cccaataggt tgttctgtaa tgatctggcg gatcagtgct tgtcgctgtt
     9361 tctttttcat attagacacc tctcaatgtt gattatagca aaagataagt aatatttctt
     9421 catattttgg tattttttac ttttattttt tgttttcatt caggttgcta acctttttct
     9481 attttaacac gcaaaatcgt cttcattttt tcaggtgctg attttcttgg atcgctgatg
     9541 taaatctctt tgtgttcttt agaagctctt tcatagcctt cttttgcgat gaatgcttct
     9601 aatttctcaa acgttgctgg ttcttcggca tagggcccga cgtgtaagat ttgggcacaa
     9661 agaccttctt ccatcttttt gaatactact tgttctttca agtcttccgg aagtttgcta
     9721 gaacgctgaa tggcttcttg tgctatcgct tctgtaacaa agtcaggctg tcggatcatc
     9781 aattgataag aaaaatgttc ttttttcatc actggttcat gcaaatactt ttcttgcagt
     9841 ccccattgtc cttccagtgg atagacagta tacatctgat aatcaggaat cagccaattt
     9901 tttttaggcg acatgcgtat tgcatagctg actgcatata gacaggctac tcttcgttta
     9961 aagtcttcct gattcgggtc gccgatccct ctgatgctaa agaatgattt tttaggaacc
    10021 tctagtattt gcgggctttt tttcatacta tagctcttgt ctttacgcca ttcaattttt
    10081 tccatttttc ttcctccgcg aaattccatt tagttattta ttatcatacc tctttttgag
    10141 tgacttggct aatcgtgttg acactatttt tcagtttgtg ctagaataag tgaagtaaat
    10201 aaatcgtaca gataacacgt ttcggcgtgt cgagagagag gcggtttcta tttttataat
    10261 agaaatcact ctcttttttt acgaaatttt ttagaggtga tgtaaatgac aaaacaaaaa
    10321 cagctgcttt tagtacctgc agtaattgca acagggatga tgtcttttgc gggcgtattg
    10381 atcgagacgg ccatgaatgt aactttccca actcttatca gacagtttga tttaacgact
    10441 gcacaagtcc aatgggtaac aacgatttat cttttgatga tttcgatcat tgttccgctg
    10501 tctacttatt tgaatcgcaa ttttagtctc aaaaaactct ttttagcttc aaatctgctt
    10561 ttcttggcag gtataatgat cgatttcttc tctcctacct tcatggtcct attgtttggt
    10621 cgattgctcc aaggtgcagc gacaggtatc gctcttccgt tgatgttcca tattatcttg
    10681 acatttgccc ccctcaatcg acgaggcgcg atgatgggtg tcggtacttt gacaacggcc
    10741 attgctccag caattggccc cacttatgga ggcattatga cttcacattt tacatggaat
    10801 catatcttcc tgtttcttgt gcccgttttg tttctttctt tgattatcgg ctggtttgct
    10861 attcccgaga tggcagtaca aaaaagtggt tatttggatt ggataagcgt tttaggaatt
    10921 tgtcttttgt ttagtggaag tctaacattt ttgagtacgt tgggaacagt ttccagctgg
    10981 attgctttaa tcatcggcgg tattggctgg ttcctttttt actatcgaag taaaaaagtg
    11041 gagcatccac tgatccgact gtctattctt tcaaataaaa cattccgctt gtttttgatt
    11101 ggcttcttag tttatcagtt tttattactt ggtgtatctt ttgtcttgcc gaatttcctt
    11161 cagatcgttc aaggaagtag tccttttgtc gctggattag cgatgcttcc cggcgcagcg
    11221 gttggtgctt tgctttctcc tttttccggg aaaatgctgg atcaatatgg tccgaagaag
    11281 ccaatttttg ctgggctgat tctttctttg attggttggc tagcattatt cctactcata
    11341 ggacacgctt cagtgggtgt attgattgct tgtcatgtgt tttatatgat cggtatcgga
    11401 ttatcttaca gcaatatgat gaccaccgga atgaatgcac tgaatgaaga actacaagga
    11461 gacgggaacg caatgttcaa taccttgcag cagttttctg gcgctgtggc tacttcatta
    11521 gtagcggtca ttatcaatta cgtccagcat catacttccc ataactatga agtgtctact
    11581 acattaggat caaaagcagc attaggcgtc ttgttgctgt tgcttctcgt aagctttgct
    11641 cgattcgcct attatctgtt ctttgctaaa aaagcgtaga ttccatatac ggcaggtgtt
    11701 gcatctgccg tttttttgtg tgaaaatggt tgtttgtaaa aggaggcttt tttatggaaa
    11761 aaattgccat tatcggtggc gggatcattg gtatgaccct agccagttac ttagatacat
    11821 caaaattcaa tgttgcttta tttgatgaag ggacaggaca ggcaactaaa gctagcgctg
    11881 ggattatctc cccttggttg tcgaaacgca gaaacaaaca atggtatcgt ctggctaaag
    11941 acggcgctgc ttttttccct aaacttataa aagatttcca acttgatcaa acgatttacg
    12001 cacaatcagg gacacttttg cttcgacctg aaaaagattt agctgaattg gctgttcttg
    12061 ccgagaaacg aaaagaagaa gcgccagaaa ttggaaacat cgaatggctt tcacctgaac
    12121 agacatctca agcacttccg ttattgaaac cagcagtttc actgcgtatc agtggtggtg
    12181 gtcgtctgga tggaaaagca tatttagaac atcttggtca gcgtttagca caaaaacaaa
    12241 tggagcagat ccaggcaaaa gtcactttgc ataagaagag cgattcttgg ctcatcaagg
    12301 gaccgtttgg agaatacaca gctgatcgtg taattttgac acctggcccg gcacttaagc
    12361 ctttattaca agaattaggc tatgctgcag atatacgtcc gcaaaaaggg cagctcttag
    12421 catttcagac atcttataag atgagtcaag actggcctgt tgctatgctt gacggagaag
    12481 ctgatttcat tcctttcatg gatggaaaga tccttttagg tgctacccat gaaaacgatc
    12541 aaggctggga tttggctcct actagcgaag cttataaggg attgactaag agtacagaac
    12601 agttcttgac tgatccagat cagataaaaa actggccata cgagttccgt gtaggtaccc
    12661 gtgcttatac gtcagatttt tccccatttt tcggcgtact gccagatgat ccaagcttac
    12721 tggctgctag cggtctaggc tcttctggct tgaccacagg tccttatatc ggctatctat
    12781 tagccaactt cttaaatact ggtcattggc ctacggaaga ttatcaaaag gacatctcca
    12841 cctatattac gaaaaatcca tcatagaaaa acagcttttt caagtaagga tcccgagcga
    12901 tattgcgcat ggattacttc ttgaaaaagc tgttatttta gttcaatttc tgaatcaaat
    12961 tctccaaact ttcttttgct tcatctggca agagatcttt tcctatctga gcttcaatga
    13021 atcgtaaacg aatatcaagt cgttgtttca ccagattttt ataggacgta tcctcaaaag
    13081 aaacaggaaa atctggtata ggtaaatagt ggatgtcttc aaatggacca aacgacttga
    13141 attctgccgt ttgttgcacg attctttcaa cgattgacaa tgtcgtttca ggtttgattt
    13201 ttttatcatc gaaatatcca gtattcagga catagcaggc tgtttgctgt ttcaaaaaca
    13261 actctcgaaa agcttgatag tcttccctaa gaggatagac acggaaagga ttagcaaatg
    13321 gttcaatgac tagctcatct agctgtcttt ttgtagtgat attttccgcg tctgatcgtt
    13381 ttgttgctaa tgttgcacca aaagacgctg ctaaaactgg gtcattgatc ttgataattg
    13441 gcggaagcga atcatctttc atgatccaaa aaacagcatc gatcttttcg ctaagatgat
    13501 cgactcggtc gggtgtcgca taacgtgatt tgactgtccg cccatttccg tttcgtatat
    13561 ctcctgttac taggactttt tctcctttac ttgtacatgt cacgcccaca ttctgacaag
    13621 taaggaaaga agaaatggcc ggatcatcag acggatagtc ttgtgtttta tcaaagtaag
    13681 aaggttccaa ggcagtagca aaaccatttt ttcgagaaat aacgaaagca tcgtcatgca
    13741 agatcgaaac agagtgacct tccgtatgtt ttgctaaggt aatcgttgat tttccagaac
    13801 cggacaaccc aaatacagcc atcgtatagg tctggtcatt actagcatat tgtttcatcc
    13861 caccgtggca ggcaataaag ccattgcgat ttcccaatgc ccaagcaagc gtcaaagtcg
    13921 ctttttttaa ttctccaaag taacgtaatc ctagcacagc cgctgtatta tggagagcat
    13981 caaacaaaat gagtccattt ggaaatgttt catgtgtcca ttcgggatca caaaagatat
    14041 aaagatctcc ctcatcaaag gaaacagact tttgataagt ctctaagatc ttcgaattcg
    14101 ctggctgaaa attcagcaaa taagaataca aattgttctc aaatccttct ggaatcagca
    14161 aatgactttg cacagaaaat tcttcagata ggccaacgat tacttttgta tggtaaaact
    14221 ggcgcttgcg actttgatac acagcttctc ttaaaatttt ctcgtatttg ctttgttcct
    14281 tgtctaagtc ctcaaagatc cgccgagcac ttgctgttct tcccacaatg cttccatcat
    14341 tgaatactag tgtcctaaca tcagctggta aatctagttc ttcagcatgt gagattgtta
    14401 aatctgtgat gatcgttcct ggactccctt ttgctagttg ataagctgtc ttcctatcaa
    14461 aaacttcatg aacattatta ccgtaaaatg tcgtttctac aatcgatcga atactggata
    14521 acagtggatt tccacttgtg atttcgttta attcgagact ctcttttgta ctcatggctc
    14581 ctcctcattc cctaaacaaa aaaacgtttt ctttattata cattattcga gtttttattt
    14641 gtactgctgc gctgtgggaa gcaaacgagt tatttttcac acaaaaaaac cgagaacttg
    14701 aataaacaag tttctcggtt tcatcatttt ccttttatcg gcttagtacc agccgtttgc
    14761 taaccagaat gttttagcag catcccatga accataacga gatgttacat attgttctgc
    14821 tacacgttct tgattggcag ctgaatagtc gccgtttaag tatgaagcat ctaattgata
    14881 acggccgatg taacgtccgt tagtagctgt gtatgaaccg cctgattctt tttgtgcgat
    14941 ccattctttt gcagagcttg aagttggcgc tgttgcagca gctggggcag caggagctac
    15001 ttcaactgtt tcaacaggtg caacgtatgt atgttcttgc gttgctgttt cagttgtaga
    15061 tgctgtattt tctgttgttt tgttttctgt tgttgctgaa gaatcatttg aagttgggat
    15121 tgttaattgt tcaccaacat aaatgcggtt gatgtttgcg atcgaatttt tttcagcaat
    15181 tgcatcgatc attgagttgt ctcctgcaaa tttttgtgag atcttagata atgaatcacc
    15241 tgattttact gtataaactt catcagcgtg agcatttgta gttcctacaa acattgcagt
    15301 agcaccagca gcaaaagttg tacctaaaac aattttctta aatgaattca tattaaactc
    15361 ctttttttct ccgtttttta ttctttcgac gctattaact ttatcattta gaaatatttc
    15421 ataggtaaaa gaaacatgac atttaatgac aaaataacga attatttaat atttgtaaca
    15481 tttaaacaaa tatttttttg atacaaaacc ataaaagcct tgttgtataa gcaaagtaat
    15541 cgtttttcta tgttttttta tttttgaatg aaaaacgcag attctgtaac attcactgca
    15601 ataatccata aaaaaaagca aaaaaatttt tgatttttat caaattctct taaaaaaatg
    15661 taaaaaatac aaaaaaacca gtaattgtta caacaattac tggacaaaaa ctttgaataa
    15721 tgttaactat ttttttattt taactttctc cgcaataatt tctgggcatg ttccaattcg
    15781 tttataacga cttcttctgt ttctttagct aatatttcat ctaatacatc aagccatttt
    15841 tccggttctt ttgttcctag acgtccaata gcccaagctg ctgttcctct gatgacaggg
    15901 cggacatctg ttaaacattc tgaaatctca ggcaatgccg ttcgatcacc tagattagct
    15961 aaagcaatca atgcgtttct ctgcaaaggt ttcttcccac gccatgaacc agctaaatgc
    16021 ccaaattgct gtttgaagtc tttattagac atcgaaagca ttggctttaa ttttggatat
    16081 atttcgtcta cttttggttc catctcttca tgaaaatgaa aatcttttcc ttgattgtaa
    16141 gggcagacta actggcagat atcacatcca taaatgacat ttcgcatttt tttacgatat
    16201 tcttccggca tcatcccttt tgtctgagtt tgataagaca aacatcgctg ggcattcatt
    16261 cttccgtcac caagcaatgc ttttgtcgga caagcagtca cgcagcgcat acagtcaccg
    16321 caaccaaatt ctcctggttt atcaggctca aatacaatat tagtaacgat ctcaccaaga
    16381 tagacaaatg aaccgaactc ttcagtgatc aacaatccgt ttcgtccaat aaatcctaaa
    16441 ccagctcgct gtgctacagc gacatcgatc agttctccag tatctacttg cggagctaga
    16501 cgccattcct cttctttttg ccatttttct gctcttgttt ggatgaattc gatcaatcgg
    16561 ttcaaacggt ctctgagtat atcatggtaa tcgatccccc aagaagcacg agcaaactgc
    16621 ccgcgttttt catccctagg cacttcttca tggatcctag taggatacgc aagtgcaatg
    16681 gaaatgatcg atttcggctg atcaaatgtc atttctggat aaagacgctc atcgatattt
    16741 ggatgttcaa agccagaagt atggccagct gctttttgtt cttccaacga ttcttttaat
    16801 gaatcaaaag gttccgccgt ggtgaacccg attttgtcaa ttcctaatcg tttgctttct
    16861 tgtatgattt cttcttttaa tgtcatggtc ttcctcgctt gctgttaacg gctgtataac
    16921 ccataatata gtttttcttt tttcagctgg acaaactgga tctgctgatc ggcaatcttc
    16981 ttttcttcca tccatttttt tcttgcagca aatacttccc aataatttgt tgaaattaat
    17041 tttcttttgt tatctaatgc attaaaaaaa ttcaatgaaa ctggttttgt acttaacagt
    17101 tcatggagcc ttacctcttc ccatggcgaa aactcagaca gatcgcggga atacaatact
    17161 tcttcataga gatttttttg ctgtgtgtac gtgaatgttt gcgggatttt cccaggccta
    17221 gcaatttcta ctagacgatt ataaaacata ttcattaatt tgctgttggc aataaattga
    17281 cttttataat aggccgactg gatctttttg attactcctt ctgctttgat gactgaaaat
    17341 aatgcaggcc atgctttgac ggttatttcg tcgttttgtt taactaatac gccaggtgtg
    17401 aagccgactg gagaaaggaa cacaaaataa ccgataccgg tattttcatc taaaggctga
    17461 aattcatcaa acaaccattt gcctatgtac agctcttcat ctggttctgt tgtctcattt
    17521 tgatttgcta tgaataattt agctaattct gcttcaagaa attgaaggtc ttgatccttg
    17581 tcggtatttt cctgctggct gatcaatcga aagacttctt ctttaatcgt atatttatct
    17641 ttacgtgtaa aattcatagc cacaccttcc ttccttactc taactataac ggaatttctt
    17701 caaaaaaggt atctgtacga cgaaaaacag cataattttt tttcatatct cttataaaaa
    17761 atgcaaggtc tgctcctcaa gctagacctt gcacttttca gctttctttt gtttgtgaca
    17821 aaccttcatg gatcgtttca atattccatt tcatcatctg atagtaagaa tctccttgtt
    17881 ctccttcttt tgctatggaa tcagtgaaca gtttatcata gattttcaaa cccgtttctt
    17941 ttgctacccg ttccatgctt cttgagtcta cactggtttc cacgaataaa accggtactt
    18001 cttttgctct tatctgatca atgatcgctt tcatttgatc aggcgtacct tgactttctg
    18061 tattgatctc ccatatataa gctgctggca aatcatatgc tcgtgaaaaa tacttgaatg
    18121 ccccttcact tgttactaat aattttttgt tctcttcgat agaagcaaaa ctttccttcg
    18181 cttgtgtatc taattctttt agtttttcca catatgcttc tgcatttttt ttatagagtg
    18241 tcgcattttc tgcatctttt tcagagaata tacgtgcgat ttcctccaca tattttattc
    18301 cgttagatag gtctagccaa gcatggggat ctgcttttgt atgttcttca ccgctagtta
    18361 aataaagagg ttctacattt ttgctaactg caaaataatc ttttccttct tcttttttag
    18421 ccgtttccat caagttatcg aaccagctgt tacctgtttc aagattcaaa ccgttgtata
    18481 aaataacatc tgcatcgctt gcctttttga tgtcttctgg tagtacttca tattcatgcg
    18541 gatctgttcc gactggtacg atactgtgga tatctatttg atctgttccg acctcttttg
    18601 ccatgtccgc caagatcgaa ttggtagcca cgactgacaa cttttcttta cttccttcat
    18661 ccgcttggtc agaagcctgc tttccgcaac cggctaatac caagaaacta actgccaaga
    18721 ttaaaaagag tgattttttt gttttcatct atctgcttcc tttctgaaat aaaattcctt
    18781 ttgttggaga aaataaaaaa gcaaggataa aaaatgcggt acatgctaac acgatcgtag
    18841 ctcctgatgg ccaattgtaa ctataactga aaaacacacc gacaactgca cttagcatac
    18901 cgattcctgc agctgtaatg atcattttca gtaaatggtt cgttagcaag taagccgtgg
    18961 cggctggtgt gatcaacatg gcaatcacca agattgttcc tactgtctgt aaactgacta
    19021 cagccactaa tgtcaaaaag aacatcaaaa gataatggat cagcgatgtg ttcagcccat
    19081 aagcttttgc cattgtcgga tcgaatgatg tgatcttcaa ttctttataa aagaaaaaga
    19141 caaatatcaa gacgattcca ctaacgagtg ctgtcagcaa caaatcgctt tccctaacag
    19201 ctaagacatt cccaaataaa atatgataca aatctgtcgc actctgggca tatgaaatca
    19261 agataatccc taatgcaaaa aaggagctaa agacaattcc aatcgctgta tcatttttca
    19321 atcggctccg ctgagtcacg aaaccaatca ctgctgctgt tagcatccca aaagcagtgg
    19381 ctccaaagat gtaactaaaa ccaaacatat aagaaatggc tacacctgga agaactgcgt
    19441 gagagatagc gtctcccatc aaagacatcc ctcgtaaaat aataaaagaa ccgatgactc
    19501 cagaagcaag tcctacaagc atggaagtga ccaaagcgta ttgcaggaat tgataattca
    19561 ttaatccatc aataaaggat tggatcattt tttctctcct ccttttacta aatgtctgat
    19621 gatctcacca taggccaatt gaaggttctt cgatgtaaag acttcttgta ctggaccgga
    19681 agcgatcagc tgtttattta aaagaataac ctcatcgaaa tattcttcta ctttatgaag
    19741 atcatgatgg acgatcaaaa tcgtttttcc tgaattcttc aattcctgca agatgtcaaa
    19801 gatctttcgt tcacttaacg catcaatccc tacgaatggt tcatctaaaa agatccattc
    19861 tgctccttgg gctagagctc ttgcaataaa aactctctga agctgtccac ccgatagttc
    19921 gctgatctgt cttttggcat attcttccat ccctactttt ttcaaagctt gtcttgcacg
    19981 ttctttttct tttttcccag gtctttgtcc aattcgtaaa gatggatatg ttcctaaaag
    20041 tacaacgcct aatacatcaa ttggaaagga aagatccaat tcacttcgct gttccacgta
    20101 ggctattttt ttacggactg agtttaccga ctgtccagaa aaggtgactt taccagtagt
    20161 agctggtatc aactctagca aagatttcat aaatgttgat tttcctgcac cgtttggtcc
    20221 gataataccg gtaatttttt gtggatttat cgtcaagttg atagttttca aaacagtttt
    20281 cccttgataa gcggctgaaa ggtcttgaat tacaatggcc gtacgttcca aatcttacac
    20341 tcctttttag gtttacctaa ctttttattt aaatatagtt taactaaaat ggaaagcatt
    20401 ttcaagtaga atgatgaaaa agtttttttc gaattttcct cgcattttat gaaaattttc
    20461 tgtagaatca catcaagagt tcaataaggc taacctaata atgaaaaaca tgtgttgaaa
    20521 cgtttgatta ttttttcttg ttgactcaac aacgaaagga gtttttgtat ggaagaagaa
    20581 aagttaactc gaagtcttag tgcccgtcat atccaaatga tcgcactagg tggaacgatc
    20641 ggtgtcggtc tgtttatggg tgcctcctca accatacgtt ggacagggcc ctcagtgatg
    20701 ggagcctatg cgattgcagg attctttttg tatttgatta tgcgtgcttt aggcgaaatg
    20761 ctttacgttg atccttcgac tggttctttt gcaaattacg cgagcgagta catccatcct
    20821 gtggctggct acttgaccgc ttggagtaat atttttcaat atattgtcgt aggaataagt
    20881 gaagtgattg ctgtaggatc ttatatgaat tattggtggc ctgatttgcc ggcaattatt
    20941 cccggaatca ttgtcgtctt gtttcttatg ctggcaaatc ttatttctgt caaggcgttt
    21001 ggcgaattag aattttggtt ttcgatcatc aaagtcataa cgatcatttt gatgatcatt
    21061 gcaggtcttg gggtaatctt atttggattt ggtaatcatg gtcaggcagt agggatttca
    21121 aatctttgga aaaatggtgg attcttcact ggaggcgtca aaggattttt ctttgcatta
    21181 tcgatcgttg ttgcttccta tcaaggaatc gaactgatcg gaatgactgc tggtgaagca
    21241 gagaatccta aaaagacgat cattgaagct gtccagtcca ctatcggtcg aatcttgatt
    21301 ttttatattg gtgctatttt tatcattgtg agtatctatc cgtggaatca attgagtgag
    21361 attggttcac cttttgtaca aactttttct aaagtcggca ttacatttgc tgcaggactg
    21421 attaattttg tcgtgattac agcggcatta tcaggttgca attcagggat tttcagcgca
    21481 agtcgtatgg tctatacact tgccataaat ggaaagatgt ctaaaaaatt tctgaaacta
    21541 tctcgtcacg gtgcgccttt ttatcctgtc gtagcaatct caggtggaat cttaattggt
    21601 ctgatcttaa attatcttct accaatgttc atcaaccagt cagaaaatat tttcgttttc
    21661 gtatacagct caagtatttt gcctggtatg gttccttggg tagtgatttt gatcagcgag
    21721 attcgcttca aaaagaagca cgcagataaa atgcatgatc atccttttaa aatgccacta
    21781 tctccttata cgaattatgt gacacttgct tttttagcta tcgtattgat tttcatgttt
    21841 atcaatcctg aaacgagaat ctcattgatt attggactcg ttttcttgct ttacatgacg
    21901 atccattact ttgtacgcga aaaaagaacc ggaaaataaa aaaagaacga agcttcacct
    21961 tagtggaagc ttcgttcttt tttattgctt ttatcaattc aattttagcg tctcaccggg
    22021 agtcaggaaa taattctcca tgctcatccc attcagcgac aagatcgttt ctactggtac
    22081 gccagttcgt tcggctattt gatttggacc ttctccttcc aaaactacgg ttgtcgcagc
    22141 ctgtgaagtc gcgctgttgg tattagtttc agaggaatct gttgtgtctt gtgttgttcg
    22201 agcttgcatt tcagaagctt ctaccgactc tgctgtctca gaatcagaag atgttgtgtc
    22261 ttgtgtgctt gttgactctg cttgaagaga ggcgctagtc tgcaaataaa tatcggaaat
    22321 cttagcgaca tgattcgtat aattagccga taattgattg atcaaagtat ctaagtctac
    22381 accgtcttta ttttctgctg cctgatacgc tgcacgtaat tttctgatcc cttcgtcgga
    22441 gattgcttga agctcacgtg ctttttggtt agcaactgct gataaaccag ctattccctc
    22501 ttgatttccc tgaatttctg tctggtattc ttcaatcaat gcaggcgtct tttcttcgat
    22561 ctttttcgaa taatcattta cgattgcttg aagttgctct ggcaaaccat ttgctgtttt
    22621 ttcttcagaa gtttgtgtct cttttgtttt agttgatgac tcacttgttt gtttcgtctc
    22681 tgttttcttt gtcgttgttt ccgttatggt cgtacttttc gcttctgacg aattttttcc
    22741 atgaaaatat agacccgcaa ctcctcctgc tatgatcaaa aacacggcaa ctgctgcccc
    22801 aatcatgatc atccgcttac gagctgcctg tttttgctgt cttcttcggg tcatccgaac
    22861 attttctccc cactcatttt catcatacat ttttttcacc accaattttt atgtataata
    22921 gcgttatcaa acgcttcata ttcttattgt agcaaataat tgtcgctgtg cacattttta
    22981 tctttgttat ttataagaaa atttcaccag ttgagatgat tccaagattg tccgtcgatt
    23041 tctccttgca gctgcattgt tcgttcgtct atctcagcca tcagtttctt tatttcatat
    23101 aagagcgcac ggtcttgata ttctgatttt tcgcttaatt catctaattg ttggatcagc
    23161 caatcatttg tcataatgaa gttcctttca tttcttgcag tgtaaaggtc aagtcttcga
    23221 tctgctcttt tgcctcagtc agtgcttcta cgatatatgc ttgtttttca gattcttctt
    23281 cctgcatttt ctgattttcc tgtactcttt gaataaacca gtcaatccgc tgagtcagcg
    23341 atggtttgcc ttccaatgag agatactgct gataagcaat tagcttctcc caacgttttt
    23401 cctgggataa atacataaat tgtgctaaaa cgaatggcgg aagataggtc atatggaatt
    23461 tatttgcgat tcgctgatac ggcctcaaaa attcggaaat cgtatattct tcttttcctc
    23521 cagcttggta ttctttttcc gccacacctg ttgtcaatac gattcctagt tctttccctt
    23581 ttaatacatg tgctgctcgt tccaagacat tatcttgcca aatttttaaa tgagcaggag
    23641 aactatacca gtaaaacgga aactggaaaa tgatccgatc atgctgggcc aagagctgta
    23701 gttcagtctc tatatctatt tttccgtctg gataacttga ttccaaatga tgccaagtag
    23761 ctggtaaacc agcagaagct tctttgaaaa attgctgatt taaagattct tcgatcgttg
    23821 gatggctaat gatgataagt gtacgcatcc cttttcctcc tttttagtct tcgctttaca
    23881 gtgtagcata accgccacaa gtttcttttg ccgatgcttg tcattttaac cagactaatc
    23941 aggttgtcca agaaactgcg gtttgttttt ccaactgttt tttcaaggta tccaaagcaa
    24001 tattcttaaa attttcgttt ttcatacgtt tacgatagtt cgtagccgtt tctccggtac
    24061 tctttttgaa cattcggcta aatacgacta gactattgaa ccctacttga taggcaatac
    24121 tttccacgga ttcatccgtc agagaaagaa gaaacgcccc tcgattgatc cgtaccccga
    24181 tcaaatattc cttgatactg gtacccatat gatttttgaa tactcgcgac aaatggctcc
    24241 gattgaccgc taaatgctgg gcgatttcct caacagacaa tttttcagca taatgtgcct
    24301 cgatgtattt gactgtttca acagctaaag aggaatgaac cttttgtaca ccaccgctga
    24361 cctttccgcc atcttttaaa aggactgata aaaaacggta ggttaatgct gttaactgta
    24421 attcattttc caaggtttct tccgtatctt tcatgcattc taaaataatg tcgatatatt
    24481 tttgtatgtc agaagagacc atcgtgtatt ggtcttctct gaaaagcgac tgccgaatga
    24541 tttcttctgc agcactccca ccaaatgaaa gccagcaata catccacgga tcttctccat
    24601 ctgataagta aaacgtcgag tctcctggac gaatcaagaa cagatcacct tttttcaatt
    24661 ggaattgttg atttttcaca tgataagatc ctttgccttc catcacgaca tgcaggatgt
    24721 aatcattgcg aatagcagga ccaaaagaat gaaacggcat cgttttagag aaaccacaaa
    24781 aatcaaaata caattctttt tctacttgtt gatgttcctt ttttaaaaaa atcgattgct
    24841 ccacgtctat ccctccttac tcctttagta taatgcaaca aaaagttaaa taaaagtcac
    24901 agaaatatat taaaaaggaa gcgatttctt gttaagttta agtcattaaa agatctttgt
    24961 tattcgttgt aaccggttta cttagtatta tatattgaac aggagaggaa tacagattat
    25021 gaaaagcaaa atgatccaac gtgtgtccta cgctttaggc gcttttggac acgacacatt
    25081 ctatgttaca ttaagcactt attttatggt attcgtgaca agccagatgt tcgcaggaac
    25141 agataaggca acgaatgcaa aaatgattgc tacagttaca agcttagttg taggtatccg
    25201 cttagttgaa attatttttg atcctttgat tggtggcatc atcgataata ctcgtacaaa
    25261 atacggtaaa ttcaaaccat ggttagttat cggcggcgtt gttagttccg tactcttggt
    25321 agcaatcttt accaatttct ttggcttagc tactcataat acgactttat ttatcatatt
    25381 atttgcaatc gttttcatct tattggatag tttttattca tttaaggata tcgctttttg
    25441 gtcaatgatt ccagctttga gtacggaaac aaaggaacgt gaaaaatttg ctacgtttgc
    25501 tcgtttcggc tcttctctag gtgcaaatgg tactactcta gtcgttgtcc ctatcgttac
    25561 tttctttacc tatcttgtta ctcataaaca tattgaaggc gcttcaggct ggttctggtt
    25621 cggtgtgatt gctgctgtta tttcaggcgg tactgcgatt atcactgcct tgggcacaaa
    25681 agaagtcgat actgcgatcc gtcagcaaaa tgaaaaagct gggatcaaag atgtgttcaa
    25741 agcagttgct caaaatgatc agttgatgtg gcttgccctt gcttatatcg cttatgcgat
    25801 tgccaatgca gcaacaactg ccgtattatt ctacttcttc aaatatgtac ttggccaacc
    25861 aactgaattt tggatcgttg gtctagttgc tgccttactt ggtttagtag ctgttccact
    25921 attcccaatg cttacacgcg tcatctctcg tcgctacgtt taccttggag ggatcgcaat
    25981 gatgatttct tcttactttt tctttacgat cgcaggttca agtttgccaa tcgttatcat
    26041 cgggctagta ttgttctact tccctcaaca attgattttc ttatccgctt tgatgacgat
    26101 cacagactct gttgaatatg gtcaatggaa aaatggtatc cgtaacgaag ctgttactct
    26161 atcaattcgc cctttactag ataaaatcgc tggtgctttg tctaacggga tcgtagggtt
    26221 tgtcgctatc gcttctggga tgactggtaa tgctactgct gctgatatca ctgctcatgg
    26281 cgtaacgact ttcaaagctt atgcattcta tgctcctgct gctttgatga ttttctccgc
    26341 tattattttt gcttggaaga ttacattgac agagaaaaaa catgcacaaa tcgttgagaa
    26401 attggaaaac aaattggctc cagctgatac aatggaagat gaacttcacg atgcagtaga
    26461 tccttcccat taagaaagga tcttgaaaat gaagattaca gaaaaagatt ttggccaagg
    26521 ttatcacttg ataaccttgg agaacaaaaa caagctagca ctttctatct ctgatttagg
    26581 tgctagaatc gtcagtttaa aatcaaatga tcgagaatta gttttgggat ttgacaccgc
    26641 tgaagaatat atcgaaaaag atccgtatat cggtgcttct atcggtcgca ccgcagggag
    26701 aatcgaaaat ggccgctttt ctctcaatgg aaaaacttac caattagcca cagatccaaa
    26761 aactggacat aatctgcatg gtggtgctcc tggatttgaa ttaaaaaaat ggtcttatgt
    26821 cattttgaat ggcgaaaatg aagcttctgt catttttact actacaagtc ccgacggaga
    26881 acatggtttt ccaggtacaa tggatgtgga aatccgctat actttgacaa aagataatat
    26941 ctggcgagtg acctctcgcg gcaccagtga ccaagatact ttattcaatc cgacaaatca
    27001 tgtttacttc aacttgacgg gagatgcttc tcagtcaatc gatcaacatg agctttggct
    27061 caatagcgaa gcttatgctc ctttgcgaac agatagtatt cctatagggg taaaagaaaa
    27121 tgctgcagga agtgctttcg attttcaaat acctaaaaaa ttagcgtccg tttttgctag
    27181 tgatttggat caaaaaaatc ttgttgacgg aatcgatcat ccatttttct taaaagaaac
    27241 cggcttgggt aaagaagccg ctcgcttgac tagccccgat aaaagaatcc aagtagatat
    27301 tgctacagat gcttctagtg tggttatttt cactgcaaat ttcggcacag aaacaccaga
    27361 aatgcgcaac cgaaaactgg cacatcacgg tggcatcaca tttgagactc aaacagctcc
    27421 aggtgcagag cgcttctcag catttggttc gattcattta aaagccggat cagtatttga
    27481 aacagtaaca gaattcaaaa tcaaaacaag aaaggaataa ctcatcatgg aaatcaaaag
    27541 aagcttaaac acaaaatttg ccgaaacatt cggacctaaa aaaactacac aatacttctc
    27601 accaggacgc atcaatttga ttggtgaaca cacagattac aatggaggcc atgtcttccc
    27661 cgcttccatc acttatggaa cgcaaggtgt agcagcacct cgagacgata ataaaatatt
    27721 agtttattcc acaaactttg acgatatcgg tgtcattagt tttactttag atgagctaga
    27781 atatgataaa aaagcaaact gggcaaatta cgtaaaagga atggtcctta aattaaaaga
    27841 agctggacac tcaatcgatc atgggtttga gttattagtg gaaggaacaa tccctaatgg
    27901 tgcaggactt tccagtagcg cttcattaga attattagta ggtgttgtat tagaagatct
    27961 gtttgattta aaaacagatc gcttggaact agtccaaaca ggtaaaaaag tagaaaatga
    28021 attcatcggt gtcaattcag gtatcatgga tcagtttgcc atcggcttcg gtgagctaga
    28081 tcatgcaatc ttattagata caaatacgct gaaatacgaa atggttccag tgaaactaga
    28141 tggttatgca atcgtcatca tgaatacgaa taagcggcgg gaattggcag attcaaaata
    28201 caatgaacgc cgtagcgaat gcgaagaagc cttggcacgt ttgcaaaaaa ctttgcctat
    28261 ctcttcttta ggagacttag atgaagaaga atttgaatca actattgacc aaatcggcga
    28321 tgatacattg atacgcagag ctcgccatgc cgtttctgaa aatcagcgga ctttgaaggc
    28381 aaaagctgaa ttagaagctg ggaacttaga agcatttggt caactgttaa atgattctca
    28441 ccactctctt cgctatgatt atgaagtaac tgggatcgaa ttagatacac tagtagatgc
    28501 tgctcaaaaa caagaaggtg tcttaggtgc acgaatgaca ggagctggat ttggtggctg
    28561 tgcgattgct cttgtcaaag aagaaaatat acctgccttc aaaaacaatg tctatgatga
    28621 atacatgaaa gttgtcggct atgctccgga attttatgtt gcacacattg gaaatgggac
    28681 aacaaagatc gatccagaag ctgttcatgt ttgataaaaa aatcaacgat ctcagctaaa
    28741 aacaaataga taaaaataac ctctcagcaa tttccaaatg tgagcctttt gctcgtattt
    28801 gaaaattgct gttttttatt gacacggaaa gagaacgtgt gttctaattg tgttgaagag
    28861 gtgattgtta tgtgggcgaa tcaaaatccg gtatttgttt atgaaaaaga accttcaaga
    28921 gatattttat gtattgactg caaatctttt tacgcctccg tagagtgcgt tgaaaaggat
    28981 cttgatcccc ttacaactaa attagtagtc atgtcctatc cgtccgattc gacagaaaca
    29041 cggggtagcg gattaatctt ggcttccagt cctgcagcaa aaaaagctta cggcatcacc
    29101 aatatcagcc gtgccagaga tcttcccttc ccctatccaa gtgatttata tattgttgcc
    29161 ccaaggatgg cctattatat ggaaaagaac agagaaatca attctatcta taaaaaatat
    29221 gcagacgaag cgaaccatca tgtctattcc gtcgatgaaa gcttccttga tgtaacaaat
    29281 agcatggcac tctttggtaa aacaaatgcg tacgacatgg ctaaactgat ccataaagat
    29341 gtgtttgagc agaccggaat ctacacgact ataggaattg gcgataaccc gctacttgct
    29401 aaactagcct tagacaatgg ctcaaaagac ggaaagcaaa tgtttgctga atggcgttat
    29461 gaagatgtac cagaaaagtt atggaaaatc tcagatatca ctgacttttg gggaatcggc
    29521 aaacgaacag caaaacgtct gaaacaaatg ggtatacatt ctgtttatga tttagctcac
    29581 gcggattatt atcacttgaa aagcaagcta ggggtcatcg gtactcagct ttatgcccat
    29641 gcttggggca tcgatcgaag ttttttggga caagaatata caccaaaatc aaaaagtatc
    29701 ggaaatagtc aaatcctaga tagagattat acaagacgag aagaaatcga gatcgttatc
    29761 aaagaaatgg ccgaccaagt cggcactcgc ttgagacgat ctgcagcaaa aacacaagta
    29821 gtcagcttat gggtaggata ctctttaggc tatcttgatc caaatggcaa gaccgggttt
    29881 caccagcaaa tgaaaatcga agcgacaaat gctagcagag atctagcaga tgctttattg
    29941 accattttcc accagcatta ccactaccag gatattcggc gtgtaggcgt gaactgttcc
    30001 gacctcgtat acgcgacagg tttacagctt aacctttttg aagatcctga aaagcagata
    30061 aatgaagcga aagttgattt tgttgtcgat acgattagaa aaaaatacgg ctttcaatca
    30121 atcatccatg cgcatagtct cttgcaagga ggacgcgcca tcgcacgtag ttctctcgtt
    30181 ggcgggcatg caggtggtat gagtggatta gaaggtggaa aagatgagac ggacagcaaa
    30241 aaaattcatt gactataacg agtatcgaga tcggccattt ggattaaaat gggggactgc
    30301 ttttgcgatg gaagaactag tcaacggtat caaaaaaatg aagaagaagc acgcaaacag
    30361 cctcttccta aacaacaaat gacattgcaa gaaatcgata gtatactgac agaagccttt
    30421 ttatctcgta aagcagtaat cattcagctc aatttaaaag atgaatttgg aagactcttg
    30481 gatgaggtcg aagggcactt cattggtgaa gcctatgaag attactttat gattgaccat
    30541 cagccgatat tgtgggaaga cgtacggtat attgagttaa aggaaacaaa aaaatggttt
    30601 gtgacatgag aggttgtgac agaagtgatc aacttcaaaa aataagaaag gatttttcga
    30661 aaattgcttc ttaaattttt gtggaatttt ggcttatttt tcgaagaagc aacttctgga
    30721 acaacgtata ttagatatca gaggttgtga aaaagtcgct tagttttgag taacaagcca
    30781 aggcgctgat ttcacaacct ctgtctatct atccatctat tcggtaagat tcatgcatga
    30841 cgtcttcttt atttagtatg tagagccctt tttcttctga ccattcaaca taaatcagct
    30901 gttttacatc tgcatagcct tctttcttca agttctccat aagccatgct tcagtcaatt
    30961 gattggactg aagcacttta tgctgaattt gcccttcatc tacaagcagg atcgataaag
    31021 ctttgtcttc atctgatttt ttcaatacac tcatttgacc ggcattctct aggataacat
    31081 gtttggcatt ttcaagaaaa aagcattctt gctgtcttaa gattgctcgc aactgctcca
    31141 tctcgatatg gttatttgtc aactctgtca tgttgatgac gccgtctttg attagaacag
    31201 atggctcgcc tttgacccaa cgattgacct tttcattttt ttggacgata cgttcgatac
    31261 cataaatcat tactgcccaa agcgcaatgg caaataggac atgccctaca tggacattgt
    31321 catcataggt tgattcttct aagatcccac ctaaaacaag cgtatagact aaatcaaacg
    31381 gtgtgatttc tgacatggat ttcttcccaa gcaaacgtac cactaataat aaaccgatca
    31441 acccgaacac cagtttcatt gtaattgaaa aaatcatttt ttctccccct aactatctgt
    31501 taattaaatt atatatgctt tctcacgaac acgcatggaa tatacaagga agaaatgtat
    31561 ccttttacta aaaaaaggac tgtgacactg tagtgacccc caaaagttag acttttttgg
    31621 agtggagaaa tccactcctt ttttttatgc tctaaaatga tatttttaca caaccgttac
    31681 ggttgtttta cgaaattgga cg
//
LOCUS       DAZKLM010000023        31349 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3764
            SAMEA115706913-rid22582563.denovo.023, whole genome shotgun
            sequence.
ACCESSION   DAZKLM010000023 DAZKLM010000000
VERSION     DAZKLM010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706913
            Sequence Read Archive: ERR13196647
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 31349)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31349)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287823.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287823.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,004
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,849
            CDSs (with protein)               :: 2,849
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 41 of 96
            Pseudo Genes (incomplete)         :: 52 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 15 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31349
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706913-rid22582563.denovo.023"
                     /isolate="VRE3764"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(451..1761)
                     /locus_tag="ACG842_000994"
     CDS             complement(451..1761)
                     /locus_tag="ACG842_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004650 - polygalacturonase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 28 protein"
                     /protein_id="HFQ0247555.1"
                     /translation="MRTKKGIINALNFKTNDTQQDDTIILQKAIDQGAKEHLPVFIPK
                     GIYLVGALFLKDKSHLIFEEGAVLKGRTDIEAFPEIDTRVAGVEMKWPAAILNVLSAK
                     DILIEGKGIIDGQGDHWWELYWGKDQKSGTRAEYDQKGLRWIADYAIKRPRACLLYHA
                     EHVVVRDLIFQKSGFWNLQITYSNDVLVEKVIIRNNDGPSTDGIDIDSSTNVRVYECD
                     LACGDDCIAIKSGRDGNGARVNRKSSRIEVARCKIRSGYGVTIGSEVSAGVSDVYIHD
                     IDFFQSDCGIRMKSSKERGGVIENIRVENLNMIDVQFPFSWIMDWHNEYNRKNSNDLA
                     QMPESWQAVAEEIPESKQMTKVKDIHIKNVQACLSPGYSLPSRAFDLVAFPEKPIEDV
                     CFSHCQITAKEFGRIEAVQNLCFKSCDLSIETGNTAANNTFDNR"
     gene            complement(1773..3131)
                     /locus_tag="ACG842_000995"
     CDS             complement(1773..3131)
                     /locus_tag="ACG842_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFQ0247556.1"
                     /translation="MSDKIGYMFGDLGNCFILGLVNSFLMIYYTNVLGVSGAIVGTLF
                     LISRILDAFADVMVGRLCDVSELTETGRFIPWIRKMKYPFCLVTVILFLPFVQHLPMT
                     VKIAYIFVSYIVYGIFLSTINIPYGAMAAAISSNPNDRTSLSTFRSIGSAIGGSTTGF
                     FIPILMYTHNKSGQQVVSGNHFFWISIGCAAIAFVAYMLTCKLTTERVRVVKNEKVSA
                     SQLVKGLLANRALIILVIVDIFIVINQILAGTNMTYLFNDYFHNKQAMSVALLFTYGT
                     VVVLAPFATKLTKLFGKKEASVFALLASSCMYLLMYFLHITNAWVYLILLFIATLGSG
                     LFNLMVWAFITDVIDYHQYKTGLREDGTVYGVNSFARKVGQACAGAVGGFMLALIGYQ
                     SSTTGGAIQSVLVQERIYTMANLLPAFCLFVSAIILLVGYPLNRSKTVFIGEELKKIN
                     ER"
     gene            complement(3333..4901)
                     /locus_tag="ACG842_000996"
     CDS             complement(3333..4901)
                     /locus_tag="ACG842_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HFQ0247557.1"
                     /translation="MQTIKVKNPILRGFNPDPNILRVKERYYIIVSSFEWLPGIRVYE
                     SKNLVNWSHTTDILTTQVSLQGNPTNGSIWAPQISYADGLFYLVYTDVKSAGRPFKDV
                     HNYLITASEITGPWSEPIYLNSNGFDPSLFHDLETGKKWLVNELWDYRLDTPNKSSGI
                     VLQEYDAEKKKLDGPIYKIFAGTELAKTEAPHLYFRNGYYYLMTAEGGTGRGHSVTVC
                     RAKNITGPYELDPGFPMLTASDKPASTLQCTGHGSLVQTPSGRWYMTYLCTRPLNGAA
                     ILGRETAIQEVYWTSDDWLRLKDSQTTPMEEIMIETNEEVQQEKDHQFMDTFEGELKK
                     NWNARRILPNADWCDTKCRPGFLRLISGESLQSTFEHHLLAIRQTDFTFDAETAFEYM
                     PNNFNQMAGLVLYLNESNYIYCYVTWNEDKGKCLRMIESENGVAQIEDWTIPLTEKKT
                     FLKVTVKKETAQFHLKDVSTEEWKKVGPQKNMCILSGGFTGNFIGIAVHDLNKKRGSY
                     ADFEFFKYDGKDHL"
     gene            complement(4905..5315)
                     /locus_tag="ACG842_000997"
     CDS             complement(4905..5315)
                     /locus_tag="ACG842_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HFQ0247558.1"
                     /translation="MKRKWELLLGMVGGSLSLIFFGGLAVTLSNMSASEFKKSYQSLA
                     VDHSTLSLENTFGLLQDMTGLFAVVLFISLAFLAVALFLTAKGKYLTTATGLYFITGF
                     ILLIGTQFIAFPFAFFYFAAGAFSLYRVRMRKGA"
     gene            complement(5312..6235)
                     /locus_tag="ACG842_000998"
     CDS             complement(5312..6235)
                     /locus_tag="ACG842_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030599 - pectinesterase activity
                     [Evidence IEA];
                     GO_process: GO:0042545 - cell wall modification [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pectinesterase family protein"
                     /protein_id="HFQ0247559.1"
                     /translation="MRMKDKLIIGKNAIDTPTDFETIQEGIDYLAGLPDSHPKVLIVK
                     EGRYDEYIESRLSNFKLVGAGTVVISGNRFAKQEYLDGSQLGTFRSATFFLEGSNVVL
                     ENLHIENTAGPGEKVGQAVALFNSGDRVSLKNCRLSGYQDTLCTGPLPDLQKDGTPFC
                     TPASANPEYCRQTYEYCWIEGTVDFIFGGADAVFHQCHIHSLASKNPGYITAASTPKN
                     QKTGYRFESCTLTAEPDTRNVYLGRPWRPYANVLFEACKIGGHVHPTGWDNWDDKKNE
                     QTVKFSERNNNYETSIKRPEWIHLEKQGVQK"
     gene            complement(6232..7110)
                     /locus_tag="ACG842_000999"
     CDS             complement(6232..7110)
                     /locus_tag="ACG842_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HFQ0247560.1"
                     /translation="MQLNDFLGAPSSRHTPSGKRLYREKQNGFMLEYYQLYLNELETV
                     PALYAYPEKEGPFPPVLYLHSHGGDFSVGKSELIHGAPYLASPSFAEVLTGMGYAVWS
                     IDAWGFEERGGISESELFKQFLLTGKTLWGMRIYDVISLIDYLETREDTDTQRLATIG
                     LSMGGLLSWWVTALDSRVKVCIDLAAQVDIEILLLHRRLDHHGFYYYVPNLLTAYTTL
                     AIQEKIAPRPRLSLVGKNDTMCLDEGVLKLRKGLDKKYQSMGSPENFSSFSLTGGHME
                     TQEMRNIWKQFIREHL"
     gene            complement(7097..8434)
                     /locus_tag="ACG842_001000"
     CDS             complement(7097..8434)
                     /locus_tag="ACG842_001000"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HFQ0247561.1"
                     /translation="MYDILTFGASIDELNTEAIQQAIDAAASDGGGTVVVPAGEFLTG
                     ALFLKSNVELHLSAGAVLKFSDDPKDYPVVHSRWEGVHRKVYASCIYAQNVENISVTG
                     FGTLDGNGKKWWHTFRNEPDNLAYPRPKLMSFHNCHRITVKDIKLIQSPSWTINPILC
                     SNATFDNLTILNPADSPNTDGIDPESCKNVRISNCHIDVGDDCIAIKAGTEDTYERIA
                     CENITITNCTMVHGHGGVVLGSEMSGSIRNITISNCIFQETDRGIRLKSRRGRGGIVE
                     DIRVSNIVMDNVMCPFILNLYYFCGPRGKEPYVWEKKAYPIDERTPAFRRIHFSNITA
                     RNVHASAGFIYGLAEQFIQEITFDNIDVSMAQDAVPGVPAMMTGIEEMSRQGFYIGFA
                     GKVSFNQMSIENHEGPAFLLENTKEISIEKFQSNQSENMDELVVRKNFAYAVE"
     gene            complement(8437..9540)
                     /locus_tag="ACG842_001001"
     CDS             complement(8437..9540)
                     /locus_tag="ACG842_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HFQ0247562.1"
                     /translation="MENWSKKLAKSIMERTPRLYEEKWYKGKWSYDYGVVLKGFQLLW
                     ERTQEKIYFDFIKDNIDYFVQEDGTIRGYSVEEYNIDHVNTGKLFFLLYKETGEEKYK
                     KAAELLSQQLANHPRTSEGAFWHKEIYPYQIWLDGLYMGSPFYAEFIQNFSEEKDYQD
                     VLRQFEISYKHLFDPKTNLLIHAWDEKKVQPWADPKTGLSHHFWSRSIGWYLMAAADT
                     YELLHEETDCSLLKEILEKTLAALLPYQEENSGTWYQVTTETKRKGNYLEASGSSMFV
                     YAMAKGIRLGLLNKEEWLPQVKKSHQGLLDEFVLETKEGWLNLNKNCQVAGLGGADQR
                     DGSYAYYISEPIICNDQKGVGAFLQALIEVEAL"
     gene            complement(9614..11122)
                     /locus_tag="ACG842_001002"
     CDS             complement(9614..11122)
                     /locus_tag="ACG842_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFQ0247563.1"
                     /translation="MKKATLVKLGLSFAGILALAACGSSSSNSSSETEDGKAKITWLN
                     ILHHASPPTDTIVNLIEEKTDTELTFNWIPDASKEERLTTALASDELGDIVTLQIAML
                     KNSSVRNALKSGVFWDVSEYLKDYKNLSQISEDRIEAASIDGALYGVPIQKDYARAGL
                     VIRKDWLDNLGLDVPTTMDELYEVAKQFTENDPDGNGVDDTVGFGDRAPSDIRYTSFK
                     LFTSYFGAPNGWAVDDNGKFTPEFDTEEYMKALNFSRDLFENGYLAQDFAVTQKTDQQ
                     EQFAQGRTGIYTGMVDIKNLKTMGQGIQDDMELVPVNKISDGEGGGYHVWSEGNGIGG
                     LLAFPKSQVKDEAQLKKLLQFVDDLLEEDVYKLMTGGIEGTHYELNDDGSIEFIDMDL
                     WQQEVQPLSSSRPSEVTFSFKDTDPEKEFSNQLIKENEEFAVMDPSVPLDSPTNNEVG
                     SEIEKIVIDATVQYIMGQLDEDGFKKAVEDWKAQGGDRITQEYEEAYKAAAQ"
     gene            complement(11088..11780)
                     /locus_tag="ACG842_001003"
     CDS             complement(11088..11780)
                     /locus_tag="ACG842_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HFQ0247564.1"
                     /translation="MLQSLDKINGRLNRILQLVYLNFLWGGLVIAGLGVFTFGPASYA
                     MVAVIRKWLRSKETFPLTKTYFTYFKENYRETLLMSWIYGAIGIVLLVDLSFIHNWYL
                     RIALYIAAFIYVLSALYIFPIMAHYDWKGYFFKMKMAFLFGFSHLHYSLVLILSMVGI
                     YLMIIQTMPGVLTFIGGSLFFSAVTWTANSIFCHLEWKNSYTEKTEENKNIIKTVKEK
                     IYEKSNARKIRS"
     gene            complement(11802..12776)
                     /locus_tag="ACG842_001004"
     CDS             complement(11802..12776)
                     /locus_tag="ACG842_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0247565.1"
                     /translation="MESVKKQEPFQLTLSKKEEKLRRRKKIIAGIKTNKFLYVMILPG
                     VLYFLIFRYLPMGGLVIAFQDYQPFLGIMGSPWVGFKHFIRLFTEPTFFTLLRNTLIL
                     FGLNIFVFFPMPIILSLLLNEVTNKRLKNGIQTIIYIPHFMSWVIIVSITYVFLNVDG
                     GVLNEFLATMGFEKISFLTSPEWTRTVYIGQVIWKELGWSTIIYLSAITAVDTQLYEA
                     AEMDGAGRLRKTWHVTLPAIRPVIITLLILKIGQTLDLGFEHMYLLLNSLNRSVAEIF
                     DTYIYTAGLKNGQLSFSTAMGLFKGVVGLFMVILANKIAKKLGEDGVY"
     gene            13076..13948
                     /locus_tag="ACG842_001005"
     CDS             13076..13948
                     /locus_tag="ACG842_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0247566.1"
                     /translation="MKKKVSIGERSFTLFNNTFLILLALICIVPFLNIIATSFASTQE
                     VVAKKFILFPTTFSLDAYRYILSTPTIFRALAVSIGVTGVGTIVSMCATSLMAYGLSR
                     KYLFGRGFVNFLVVFSMLFSGGMIPTFLVVRSLGLVNSYWAMILPVAVNAMNMIIMRN
                     FFQALPDSLEESAKMDGCTDFGVFFKIMLPLALPSIATISLFYAVTYWNTYMTAILYI
                     NDSSKWPIQILLRQIVIVSSGMQAESSAVDVIPPAQTVKMAVIVIATVPMLIVYPFVQ
                     KYFVKGALVGSVKG"
     gene            complement(14406..16604)
                     /locus_tag="ACG842_001006"
     CDS             complement(14406..16604)
                     /locus_tag="ACG842_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0247567.1"
                     /translation="MKKSTVFYKIFLPMFLLGAVLVIGFSLFIYQNTYESIESSYLTD
                     KKNLLRQVKTNVEWKIRTIEYSFSTYGSTKNFSDIFKHPLTYTDYSTYSEIRKELNFI
                     ETIVMDDNNYDLVSLAGKWGVINGSLSQLTEEEIEKYQQKYINNKNNLFWQKKNKGIE
                     MVISLPMFEKEKYALGIATIEKHTIEQVVGESGEDILSIENEVGNLFTTQTVKNKEQP
                     TVLTDVDLDYNQLKVIREKDKTYIVLKSDYNNWIYRLEVDSSAIGAMIRNLRIGLVAV
                     SLTLVGLIGLLSYLFSDRFARPISQIQERLNLKSKGFSGKELDLVAQSVSRIIGEKEA
                     LSAHLVTQKPQLETLFVLSLFRNRVERRELDQRLQQFGYSSQNDCYYTALVQIDLLED
                     SHGGERDLLLLAINNMITEIVPKENRMLPIVLNEEMQATIYRMDKDDAEASKKVMEYC
                     RLIQKRVKEYLNLTISIGISNRFETLNESKQSVDRAKEALYYRVNTGPSSIIFYQEIV
                     PAAHEKTLIRYPIEEQNRLFEAIRSGNQEVKNLVHELIDALFAQNKNPLSREVVSVRL
                     INELVQLGQLLGVDSKNFDNMKQIYVKALNNYHPKELEKLVLEQLVLPITECSQAITD
                     HEFKNLSENIMHIVYTEYDRDLSLDVIADRLHYNPNYLSNVFKKETGEKFGDFVQNHR
                     LEVAKKWLKETNLSVKEIAERLQYRNPQNFIRFFKKKESITPGEYRKKYL"
     gene            16836..17828
                     /locus_tag="ACG842_001007"
     CDS             16836..17828
                     /locus_tag="ACG842_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFQ0247568.1"
                     /translation="MVTIKDVAKAAGVSHTTVSRALNNSSLIKNETKQKIRQLAKEMN
                     YSPNYSAKGLVNQKKYLIGLFFSSLHKGTSSNFLADSIAGIHSVLDTNYSLSVESIDV
                     VDLTEINLQRYDGLIVMSQSDDDQPFIDYLKEKEFPFVVVNRHINDPAVINVTADDAA
                     GVMQAVDYGIGCGHTKIAYIGGKKNFRSTKEREKGVLKSLKKASIPVNPAYFLTGDYS
                     LESGLQNMEYLLSLSDLPTLVFCGNDDIAIGALRAVHLAGLRVPQNISLIGFDDSPVV
                     SYLNPPLTTVRKPLKEMCQEGTKLLLDLIEGRTIEQSKIELSTLLILRESINYL"
     gene            17888..19309
                     /locus_tag="ACG842_001008"
     CDS             17888..19309
                     /locus_tag="ACG842_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HFQ0247569.1"
                     /translation="MKKLTKELVKKNEAPVKVLQFGEGNFMRGFIDWQIQQLNNQQLF
                     KGNVAIIQPLEQGLGNMMNQQDNLYTVILQGLSNGEIVDTSEIITVVEQMINPYDEWD
                     AYLSLAENDDLAYIVSNTTEAGIQFVETDNLTDTPPQSLPGKLTAFLYRRFKLNKAGF
                     TIIPCELIDRNGERLKEIILRYAEKWDLEPAFVEWLEKENVFCCSLVDRIVPGYPRED
                     AGVFNQRHNYEDQLMVKAEPFMLWVIEGPKELENQLPFKQAGLNVIITDDMTPYRERK
                     VHLLNGPHTAMVPLALLAELETVEEVMKDPLFSAYVDQLFNLELIPMLSLPKDELAIY
                     ADQIKERFLNPFAHHKLEAISLNSVSKFSTRLLPVFKKYIEEQNQVPPLITVSLAALL
                     LMYRGDQVKPHDDEKTISEFTDAWSDNGTAIPRLLQNAALWGEDLSQIPNVTDTVQEI
                     AAKIDQKGVRSVLQEMKGTMVHA"
     gene            19302..20801
                     /locus_tag="ACG842_001009"
     CDS             19302..20801
                     /locus_tag="ACG842_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HFQ0247570.1"
                     /translation="MLETLQPTMKLNRRDSVVVALTPIPKQTQLTIDQQTITTLEDIP
                     QGHKIALGDLKAGDNVIKYGYPIGHVTTDVTAGQWLHTHNVKTNLSGELDYRYEKDVH
                     PSHYLFENRTFQGYLRKNGKVGIRNDLFLVPTVGCVNGIAELIVKQFKEKHPDLGQFD
                     HITILKHPYGCSQLGKDHQNTREILADAVNHPNAGGVLVFGLGCENNTVPEFKKILGE
                     YDEERVKFLVAQDVYDEIEQGVALLEELLTAAENDQRISVPLSKLNVGLKCGGSDGLS
                     GITANPLLGAFSDYLIAQGGSTILTEVPEMFGAEQVLMARAENEEVFDSIVDLINDFK
                     QYFLSYGEPVYENPSPGNKAGGITTLEDKSLGCTQKSGSSPVVDVLQYGEKIRKPGLS
                     LLQAPGNDLVAASALASSDCQLVLFTTGRGTPFGSYVPTVKVSTNTTLFERKGHWMDF
                     NAGVLLEQPMEVILESFVQKIIAVASGEETKNEQNDVREIAIFKNGVTL"
     gene            20840..22234
                     /gene="uxaC"
                     /locus_tag="ACG842_001010"
     CDS             20840..22234
                     /gene="uxaC"
                     /locus_tag="ACG842_001010"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HFQ0247571.1"
                     /translation="MFLSDDFLLKDDWAKKLYHSYAKNMPIIDYHCHLDPKEIYENNN
                     FSNLTEAWLGGDHYKWRLMRACGVPEEKITGNASDFEKFLAWCQTVPKIIGNPLYSWS
                     HLELKRFFGIDLLINEENAEKIWTQANEILAAPDFRRREIAKKSNVEVICTTDDPADD
                     LHYHQLLAHEEPDLKVLPSFRPDKALNIEKEGFNAWVTLLEEAADQTITTYQELIEVL
                     GQRIEYFHQHGCRVSDHALDILRYKEADETVLEEIFQKARANKLLSSDEIDAYRTETL
                     IHLTHFYHSHNWTMQLHIHAYRNCNTQMLEKLGPDTGYDGMNDLSLTIPLQKLLNRAE
                     ETDQLPKTILYSLNPNDYPAILALMGCFQKEQNGKLQLGSGWWYNDTRAGMRDQLTQF
                     ADGSALGNFVGMLTDSRSFLSYTRHEYFRRVLCEFLGEMVMRGEAPEDEQLLGSLVQA
                     ISYTNAKNYFGFFA"
     gene            22393..23076
                     /locus_tag="ACG842_001011"
     CDS             22393..23076
                     /locus_tag="ACG842_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnogalacturonan acetylesterase"
                     /protein_id="HFQ0247572.1"
                     /translation="MPTIHIAGDSTAAEKDENKRPETGWGEKISAYFSPEIQINNQAK
                     NGRSTKSFIQEGRLARLASQFQPNDFLFVQFGHNDQKIQEERGTLPFGSYLKNLQIFF
                     ECAKKANVQMVLLTSVTRRDYLENGTLNPDILGDYPKAMRAFAEKHHIPLLDVFSRSQ
                     ELFQTFSKEETKKFYLHLIPDTCKNYPEGLKDNTHFSPEGADKVARIIVELIKESRLP
                     LANYLQKGV"
     gene            23078..23914
                     /locus_tag="ACG842_001012"
     CDS             23078..23914
                     /locus_tag="ACG842_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HFQ0247573.1"
                     /translation="MFPLNLGIRAHDLDVRNREEIVQKVSQLHLSHIQFAPQKAFPEQ
                     LKLTDMTLGSAAYFGEYFQKNNIELSILGCYINLSSKNQEVRAQALATFKQQIALCSA
                     YQAKMIATETGSVSVGYTKENFTEEAYQIARNSIIELVAHAENFGVTVAVEAGINHPI
                     YNYQLAKRLIDEVASPNLKIILDCANLMHKENHADQEKVIRGALDNLHGYITALHLKD
                     YIMENGKIRMVPVGKGCLDFRPVLHYIKYERPLMYATLEATPEIYVPEAIEHLQFIYQ
                     DC"
     gene            23925..25679
                     /locus_tag="ACG842_001013"
     CDS             23925..25679
                     /locus_tag="ACG842_001013"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0247574.1"
                     /translation="MEERIQKSYQKYHANAFQTLLHLYQGNYWRFAMSGLFFIIKHLP
                     SWLMPIAIANVINAASSKDSSQLQAIYFNAFLMACLIGQNILTNYFHVKYHSTSIRQV
                     EAGLRATLIKKIQELSIPYQKELQSGRIQSKIIRDVEAIQTLSSQVFVSGLNIAVNLF
                     VALGITLYKSPVVFMFFLLTVPLAAFVTLFFRKRIRQSNSDFRQEMEETSAKVIEVIE
                     LAPIARAHGLEKHETTRLNKHFTQIYEKGFHLDILQSVFGSVSWASFQIFQLICLIFT
                     GILALQGRIQPGDVVMYQTYFTTIVNSVSGMITLIPTISKGMESITSIGELLIAGDVE
                     TYHGKKKIQHLDGNFRFEDVSFLYPDSLKPIIDHFSLEVKAGETIAFVGPSGSGKSTL
                     LNLLIGFIQPTSGKIFLDDQPLDELDMRSVRNYLAVVPQTTLLFSASIKENITYGLKN
                     VSKERLDEVIEAAQLSSLIDQLPDGLDTLVGEHGNKLSGGQKQRISIARALIRQPKII
                     LLDEATSALDNQSEKKIQQALNYLTETPTTFIVAHRLSTIKEADKIVVIDEGKIVEIG
                     TYEKLLAQKGYFYRLKNE"
     gene            complement(25955..26296)
                     /locus_tag="ACG842_001014"
     CDS             complement(25955..26296)
                     /locus_tag="ACG842_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HFQ0247575.1"
                     /translation="MSNIYDSANQIEREIRELPEFKALKEAYEKVQANEEAHKLFKDF
                     QAMQIELQEKQMSGQEFSDEDAMKAQEMAVKIQSEEVINDLMQKEQGFSTIINDLNRI
                     IMTPVRDLYSE"
     gene            complement(26364..28535)
                     /locus_tag="ACG842_001015"
     CDS             complement(26364..28535)
                     /locus_tag="ACG842_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HFQ0247576.1"
                     /translation="MDFQSILEKIKAALISFWRWIRPYLGQFHRWRKRIWKKYHVNKL
                     ILLIGLICVLVTSIYLFILAKQANVETLKSGLSQSTVIYDKKNEEAGTLYAQKGTYVE
                     LNAISPYIQDAVISTEDRNFYEHHGFDIKGIARAAVRMVLSGGTSGGGGSTITQQLAK
                     NAYLTLDQTFDRKAKELFLAIEIEKKYSKEEILTMYLNNAYFGNGVWGVQDASRKYFG
                     VDATNVTLSEAATLAGMLKGPGIYNPIDHPKNANDRKNTVLSVMEENGKITKEQEQAE
                     SQTDISAYLNDTYDNSDSGYRYPYYFDAVIDEAVNEYGLDEEDVMNKGYKIYTALDQD
                     YQKQLEATYQNSALFPANASDGAMVQSASVALDPNTGGVQALVGRRGDHVFRGFSFAT
                     QMRRSPGSTIKPLSVYTPALEAGYKPSSILKDEPQSYYDAHNFDGTYQGEVPMYQAVA
                     QSLNLPTVWLLHEIGLQKGYDKAEEFGLPLAKSDKYYGLALGGLEKGVSPLIMAGAYS
                     AFANDGQMYTPHLITKIVDSTGAVIVDNTNKKPKQVISKETADEMTSMLLGTFSNGTA
                     AAANPAGYTIAGKTGTTETNFDATKANDQWMIGYTPDVVISTWMGYEESSKLHYLEGT
                     SGNVVGKVFKSAAEGILPYTSKTGFSVADAYATGGQVVAADQVGNTGNSNGESGNWQD
                     NLNQYGEQAKEGLKNFGDMVKEGVQGIGEAAKDLWRKYQGE"
     gene            28746..29603
                     /locus_tag="ACG842_001016"
     CDS             28746..29603
                     /locus_tag="ACG842_001016"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HFQ0247577.1"
                     /translation="MKITITLPMNHPETTIRDLLEKEWLVPRKVRHFLRIRKNVWINQ
                     QPALFHHEVKGNDQITLLFEETDYNYQEVQLGDAKHIRVLYEDEHLIIVDKPAGMKTH
                     PNEPTENSTLLNHLAAYLDTKQQRPYVVHRLDKETSGAVLFAKNPFVLPILGRMLEQK
                     MIYRRYQAIVWGEIAKDMTITNKIGRDRHDRRKRVVDDKNGQSALTHVTVNKVLSEQT
                     QVYCVLETGRTHQIRVHLAHKGHPIVGDPLYQNRTAERLMLHALELHMIHPFTQEKIV
                     GKALPGLWE"
     gene            complement(29674..30474)
                     /locus_tag="ACG842_001017"
     CDS             complement(29674..30474)
                     /locus_tag="ACG842_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFQ0247578.1"
                     /translation="MTIKKKLSIPTTTPEETLDQVFEFFDRYELKGIGIGSFGPIGID
                     KTKDNYGYVLATPKKGWAQFDFLGTIKERYNIPAAWTTDVNAAAYGELKQGAAQGKNS
                     CIYLTVGTGIGAGVVINEEIFSGIAHPEMGHIWVKRHPDDHYEGTCPYHKDCLEGLAA
                     GPSIEARTGIKGQDLPQDHPVWAIQAYYIAQALINYTLTLAPEKIILGGGVMNQDHLL
                     QKIRQQFVELMGGYMETPQVSEYIVRWGLPNESGIIGSLLLAEHEHQK"
     gene            complement(30471..>31349)
                     /locus_tag="ACG842_001018"
     CDS             complement(30471..>31349)
                     /locus_tag="ACG842_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFQ0247579.1"
                     /translation="AHCLNRSRQTIHKVYLFFKQGKSALEYYQQYKKNKSNCGRRPLV
                     LPEEQSEYIQRKVVQGWTPDVIVGRAAFPISCSARTIYRMFKKGLFDSSDLPMKGKRK
                     PNGHQERRGKQTFRRSIHEREKDYSQFSNEFGHLEGDTIVGLKHKSAVITLVERLSKV
                     IITLKPCGRQAIDIEKKLNQWFESVPKNLFKSITFDCGKEFSNWKQISNVNDIAIYFA
                     DPGTPSQRGLNENSNGLLRRDGLLKSMDFNSVDEFFIQSVASKRNNIPRKSLDYRTPL
                     EVFLSYVSIDDLSNLI"
BASE COUNT         9367 a         6443 c         5541 g         9998 t
ORIGIN      
        1 ttgtcttgaa ttgaatcaag acgccctaca catttggttt gtcttacttt gttcagtttt
       61 caaaggtcta cttcatcgag agtgtttccc gattcatttt tggctgccgt agcaactttt
      121 atatcatatc agagagacaa acgattgtca actctttttt aaaagttttt ttcgatttcg
      181 tttttcgaaa tctttcagca actcatttat cataacttat cgtttgttat ttgtcaagaa
      241 cttttttgaa gtttttttcg atagaatcaa gcttcgcaag tggttctttt taacgactta
      301 gttatcttat cacgtctaaa aacatgtgtc aactcttttt tggaattttc ataaaatgtt
      361 tcaatgaagc gcgacaacgt tgtttaatat atcattaaaa aaaataaagg tcaagctaaa
      421 atattattta agcttgacct tattataatt tcaacgattg tcaaaagtat tgtttgcagc
      481 tgtgttgcca gtttctatag ataaatcaca gcttttgaaa cataagtttt gtactgcttc
      541 gattcgcccg aattcttttg cagttatttg gcaatgagaa aagcagacat cttctatagg
      601 cttttcagga aatgctacta aatcaaaagc tcgggaagga agtgagtaac ctggagaaag
      661 gcatgcctgt acgtttttga tgtgtatgtc tttgactttc gtcatttgct ttgattcagg
      721 tatttcttca gccactgctt gccaactttc tggcatttga gcaaggtcat tgctattttt
      781 tctattgtat tcattatgcc agtccattat ccaagaaaaa ggaaattgta catcaatcat
      841 attcagattt tctaccctga tattttcgat cactccccct ctttcttttg aagatttcat
      901 tcgaatacca cagtctgact gaaaaaaatc gatatcgtga atatatacgt cactaactcc
      961 agcactaact tcgctaccaa tagttacacc gtatcctgaa cggattttac atctagcaac
     1021 ctcgattctt gatgattttc gattgactct tgcaccattg ccatctctgc cagacttaat
     1081 agcaatacaa tcatcaccac aggccaaatc acattcatag acacgcacat ttgttgaaga
     1141 atcaatatca atcccatctg tacttggtcc atcattgttg cggatgatta ctttttctac
     1201 taatacatcg ttagaataag tgatttgcaa gttccaaaaa cctgatttct gaaaaatcaa
     1261 atcacgaaca acaacatgtt ctgcatgata cagtaaacaa gcgcgtggac gtttaatagc
     1321 ataatccgca atccaacgaa gtcccttttg atcgtattca gctcttgtcc cgcttttttg
     1381 atcttttccc caatacagct cccaccaatg gtctccttga ccgtcgatta ttccttttcc
     1441 ttctataaga atatctttcg ccgagagaac attcaaaatc gctgctggcc atttcatttc
     1501 tacgccggct actcgtgtat cgatttctgg aaatgcttcg atatctgttc tccccttgag
     1561 gactgcacct tcttcaaaga taagatgact cttatctttt aaaaaaaggg ctccgactaa
     1621 ataaatgcct tttggaataa atacaggtaa gtgttctttc gcaccttgat cgattgcttt
     1681 ttgcagtatt attgtatcat cttgttgagt gtcgtttgtt ttaaagttca aagcattgat
     1741 aatccctttt tttgtccgca atacgtttct ccctatcttt cattgatttt tttcaattct
     1801 tcaccaataa atacagtttt tgaacgattc agtgggtaac ctactagcaa aatgattgcg
     1861 gatacaaata agcagaatgc tggtaacaag tttgccatcg tataaatcct ttcttgtact
     1921 aaaactgatt ggatggctcc acctgttgta gacgattggt aaccaatgag tgccagcata
     1981 aatccgccta ccgctcctgc acatgcttgt cctacttttc tcgcaaaaga atttactcca
     2041 tatactgttc catcttctct taagcctgtc ttatattgat gataatcaat cacgtcagta
     2101 atgaatgccc agaccatcaa attgaataaa ccagatccta aagtagcgat aaataaaaga
     2161 atcagatata cccatgcatt tgttatatga aggaaataca tcagcaagta catacatgaa
     2221 gaagcaagta acgcaaatac gcttgcttcc tttttaccaa ataattttgt cagctttgta
     2281 gcaaatggtg ctaaaactac tactgttccg taagtaaata aaagagcaac agacattgct
     2341 tgtttattat ggaagtaatc attgaataaa taagtcatat tcgtcccagc taaaatctga
     2401 ttgatcacaa tgaaaatgtc aacaatgact aaaataatca gtgctcgatt agctaacaaa
     2461 ccttttacca actgagaagc agatactttt tcgtttttga caactcttac tctttcagta
     2521 gtcaatttac aagttaacat atatgcgaca aatgctattg cagcacatcc gattgaaatc
     2581 caaaagaaat gattccctga aacgacttgt tgtccactct tgttatgggt atacattaaa
     2641 atcggaatga agaatcctgt agttgatccg ccaatcgcag aaccaatgct tcggaacgtg
     2701 gacaacgagg tgcggtcatt aggatttgag ctaattgctg ctgccattgc tccatacggg
     2761 atattgattg ttgagagaaa aatcccataa acaatgtatg aaacaaaaat ataagcaatc
     2821 ttcaccgtca ttggaagatg ttggacgaat ggcaaaaaca aaatgacagt cactaagcaa
     2881 aatggatact tcatttttct gatccaagga ataaatcggc cagtttctgt taactctgag
     2941 acatcacata atcttccaac catcacatca gcaaacgcat ccaaaattct cgaaatcaag
     3001 aataacgtac ctacgattgc ccctgaaacg ccaagaacat tcgtataata aatcatcaag
     3061 aaactattga ctaaacctaa aataaaacag ttacctagat caccgaacat gtagcctatc
     3121 ttatcggaaa tcccaaattc ctttttttct tgtttttata ttcacaatgt tggcttttta
     3181 ttgcattatc ttgacagata aaaaacaaaa aataatctaa aaacattgat gcataagatt
     3241 gataactttt tttacataac aatatataca aacaaaaaaa ttcactaact agtgaaaata
     3301 agttagtgaa cctcatttat taaagaatta aattacagat gatcttttcc atcatacttg
     3361 aaaaactcaa aatcagcata acttcctctt tttttattta gatcatgcac tgctatcccg
     3421 ataaaatttc cagtaaaccc tcctgagagt atacacatgt ttttttgcgg acctactttc
     3481 ttccattctt cagttgatac gtctttcaaa tgaaactgtg ctgtctcttt ctttacagtg
     3541 actttcaaaa aagtcttctt ttctgtgagt ggaatcgtcc aatcttctat ttgtgctact
     3601 ccgttttctg attctatcat acgtaggcat tttcccttgt cctcattcca agtaacataa
     3661 caataaatat aattggattc atttaagtac agtaccagtc cagccatctg attaaaatta
     3721 ttaggcatgt attcaaatgc tgtctccgca tcaaaagtaa aatccgtttg ccgaatagct
     3781 aacaaatgat gttcgaaggt agattgaaga gattcaccgg aaatcagacg taaaaaacct
     3841 ggtctacatt ttgtatcaca ccaatcagca tttggcaaaa tgcgtctggc attccagttc
     3901 ttctttagct ctccttcaaa tgtatccata aactgatgat ctttttcttg ttgtacctct
     3961 tcatttgtct caatcattat ttcttccatc ggagttgtct ggctatcttt aagtcttaac
     4021 caatcgtcgg acgtccaata cacttcttga atagcggttt ctcttcccaa aatagccgca
     4081 ccgtttaatg gtctggtaca aagatatgtc atgtaccatc ttcctgacgg ggtttgtact
     4141 aagctcccat gtcctgtaca ttgcaatgta gaagcaggtt tgtcactagc tgtcagcata
     4201 ggaaaaccag gatcaagctc gtaaggacca gtaatattct ttgctcggca aactgtaact
     4261 gaatgtcctc ttcctgtacc gccttctgcc gtcatcaaat aataataccc gttacgaaaa
     4321 tacaaatgtg gtgcctccgt tttcgctaat tctgtacctg caaatatttt atagatggga
     4381 ccatcaagct tcttcttctc tgcatcatat tcttgtaata cgataccgct ggatttattc
     4441 ggtgtgtcca gacgataatc ccatagttca ttgacaagcc atttttttcc cgtttcaaga
     4501 tcatggaaca aagaaggatc aaagccgtta ctatttaaat aaattggttc ggaccaagga
     4561 cctgtgatct cagatgcagt gatcaaataa ttgtggacat ctttaaacgg acggcctgca
     4621 cttttgacat ctgtgtaaac aagataaaac aatccatcag cataactgat ctgtggcgcc
     4681 catatacttc catttgtagg atttccttgc aaagatactt gagtggtcaa aatatccgtt
     4741 gtatgtgacc agttgactaa atttttcgac tcatatactc ggattcctgg aagccattca
     4801 aaagaggaga caataatgta ataccgctct ttcactctta atatattagg atctggattg
     4861 aatcctctta aaataggatt tttcactttg atagtctgca tgattcaggc tccttttctc
     4921 attcttactc gatataaact gaatgcccca gctgcgaaat aaaaaaatgc aaaaggaaac
     4981 gcaataaact gagtgccaat caacaatatg aatcctgtta taaaatacaa accagtggca
     5041 gttgttaagt attttccttt agcagtcaaa aacaatgcta ctgctaaaaa agcaagtgaa
     5101 ataaacaaca caacggcaaa cagaccagtc atgtcctgta ataatccaaa tgtattttct
     5161 aaagacaaag ttgaatgatc tacagcaagc gactgataac tttttttaaa ctctgacgca
     5221 gacatattac ttaaagtaac tgctaacccg ccaaagaaaa tcaaagatag actgccaccg
     5281 accattccta gcaatagttc ccattttctt ttcatttctg aaccccctgc ttttctaaat
     5341 gtatccattc aggtcttttg atcgatgttt cataattgtt attacgttca gagaatttga
     5401 cagtctgttc atttttcttg tcatcccaat tatcccaacc agttgggtga acatgcccac
     5461 ctatcttaca cgcttcaaat aagacatttg catacggacg ccacggtctt cctaaataga
     5521 cgtttctagt atcaggttct gcagtcaagg tacagctttc aaaacgatac cctgtttttt
     5581 gatttttcgg cgtagatgct gctgtgatgt atccaggatt tttactggct aagctatgga
     5641 tatgacactg atgaaaaacg gcatctgccc caccaaatat gaaatccacc gttccttcaa
     5701 tccagcaata ttcataggtc tgacgacaat actcaggatt ggcagaagcc ggtgtacaaa
     5761 aaggagtacc atctttttgc aaatcaggca acggacccgt gcataaggta tcctgataac
     5821 cagatagtcg acagtttttt aaagatacgc gatctcctga attgaataaa gcaacagcct
     5881 gtcctacctt ttctcctgga cctgctgtat tttcgatgtg aagattttct aatacgacat
     5941 tcgatccttc taagaaaaaa gtagcacttc ggaaagtccc taactggcta ccgtcaagat
     6001 actcctgttt tgcaaaacga ttaccggaga taactactgt tcctgctccc acaagtttaa
     6061 aattggataa tctgctttct atatattcat catatcttcc ttcttttact atcaagacct
     6121 ttggatggct atccggcagg cctgccaaat aatcgattcc ttcttggata gtttcaaaat
     6181 ccgtcggggt atctatagca ttctttccaa ttatcagttt gtctttcatc ctcataaatg
     6241 ctcccttatg aattgtttcc aaatatttct catttcttgt gtttccatat gaccgcctgt
     6301 taaagaaaaa gaagaaaagt tttctggact acccattgat tgatactttt tgtccagccc
     6361 ttttctcagc ttcagtacgc cttcgtccaa gcacatcgta tcgttttttc caactaagct
     6421 aagacgaggg cgcggagcga ttttttcttg gattgctaac gttgtataag ctgtcaatag
     6481 atttggcaca tagtaataaa acccatgatg atctaggcgg cgatgtagca aaagtatttc
     6541 gatgtccacc tgtgccgcta gatcaataca aactttgaca cgagaatcca atgctgtcac
     6601 ccaccagctt aataagcctc ccatagaaag accaatcgtt gcgagccgtt gagtatctgt
     6661 gtcttccctt gtctccaaat aatcgatcaa gctgatcaca tcataaatac gcattcccca
     6721 taacgttttt cctgttaata aaaattgttt gaacaactcg ctttcagaaa tacctccgcg
     6781 ttcttcaaat ccccaagcat caatcgacca aacagcatat cccattcctg tcagtacttc
     6841 tgcaaaagat ggagaagcta agtaaggagc accatggatc aattcacttt tcccgactga
     6901 aaaatctccg ccgtgagaat gcaggtaaag gaccggcgga aacggtcctt ctttttctgg
     6961 ataagcatag agggctggta ctgtttccaa ttcatttaag tacaattgat agtattcaag
     7021 cataaaaccg ttttgttttt ctcggtataa tcgttttcca ctaggggtat gtctgctaga
     7081 tggagcacct aaaaaatcat tcaactgcat aagcaaagtt tttacgaaca accagttcat
     7141 ccatattttc tgattgattt gattggaatt tctcaatact gatttcttta gtattttcta
     7201 atagaaatgc tggtccctcg tgattttcaa tcgacatctg gttaaaggaa accttacccg
     7261 caaagccaat ataaaatcct tgtctgctca tttcctctat tcctgtcatc atagcaggaa
     7321 caccgggaac tgcatcttgg gccatcgaga catcgatatt gtcaaatgtg atttcttgta
     7381 tgaattgttc cgctaatcca taaataaaac ctgcagaagc atggacattc cttgccgtga
     7441 tattcgaaaa atggatcctt ctgaatgctg gtgtccgttc atctatcgga taagcctttt
     7501 tttcccatac atacggttct ttccctcgcg gaccacagaa ataataaaga ttcagtataa
     7561 atggacacat cacattatcc atgactatat tggatactcg aatatcttca acgatcccgc
     7621 ctcgacctcg tctagatttc aatcgtattc ctcgatcagt ttcttggaag atacaatttg
     7681 aaatggtaat attacgaata ctaccactca tttcacttcc taatacgaca ccgccatgtc
     7741 catggaccat cgtgcaattt gtgatcgtga tattttcgca agcgatcctt tcgtacgtat
     7801 cttcagtccc tgccttgatt gcaatacagt catctcccac gtcaatatgg caattactaa
     7861 tacgcacatt tttgcatgat tctggatcaa ttccgtctgt atttggtgaa tccgctgggt
     7921 tcaagatcgt caagttatca aacgttgcat tactgcaaag aatcgggttg atcgtccaac
     7981 ttggtgactg gatcaattta atatctttca cagtaattcg atgacagtta tggaaactca
     8041 tcaatttagg acgaggataa gcgagattat ctggttcatt tctaaaagta tgccaccatt
     8101 tttttccatt tccatctaat gtaccgaaac cagtgacaga gatgttctct acattttgag
     8161 catagataca ggaagcataa acttttcgat ggactccttc ccaacgtgaa tgaacaacag
     8221 gataatcttt tggatcgtca gagaatttca gaaccgctcc tgcgcttaaa tgaagttcga
     8281 cattgctttt taaaaataaa gctcctgtta gaaattcacc tgctggaaca actacggtcc
     8341 cgcctccgtc acttgctgca gcatcaatgg cttgttgtat tgcttcagta ttcaattcat
     8401 caattgatgc gccaaatgtc aaaatatcat acatatttat aatgcctcca cttcaatcaa
     8461 tgcttgcaag aatgcaccaa cgcctttttg atcattacaa ataattggtt cactgatgta
     8521 gtaagcatat gaaccatctc gctgatctgc tccgcctaac cctgctactt gacagttttt
     8581 attcaagttc agccagcctt cttttgtttc cagaacaaat tcatcaagta atccttgatg
     8641 tgatttcttc acttgtggaa gccattcttc tttgttcagc agtcccaaac gtattccttt
     8701 agccattgcg taaacaaaca tactactgcc ggaagcttcc agataatttc ctttccgttt
     8761 tgtttctgtt gttacttgat accatgtccc gctgttttct tcttgatatg gaagtaatgc
     8821 agccagtgtc ttttctagaa tttctttcag caaagaacaa tctgtttctt catgaagtaa
     8881 ttcataggta tctgctgcag ccatcaaata ccagccaatc gatcggctcc aaaaatgatg
     8941 ggatagacct gtttttggat cagcccatgg ttggaccttc ttttcatccc atgcatgaat
     9001 cagcaaattt gtttttggat cgaatagatg tttataactg atttcaaact gtctcaaaac
     9061 atcttggtaa tctttttctt cactaaaatt ctggataaat tcagcataaa aaggagaacc
     9121 catatataag ccatccaacc aaatttgata aggataaatt tctttatgcc aaaaagctcc
     9181 ttcggatgtg cgtggatggt ttgccaactg ctgggaaagg agttctgctg cttttttata
     9241 tttttcttcg cctgtttctt tgtacaataa aaagaaaagc tttcctgtat ttacatggtc
     9301 gatattgtat tcttcaacgc tgtagccacg gatcgttcca tcttcttgca cgaaatagtc
     9361 gatattgtct tttatgaaat caaaataaat tttttcttgg gttcgttccc aaagtaattg
     9421 gaacccttta agtacgacgc catagtcata agaccatttt cctttatacc acttttcttc
     9481 atataagcga ggtgttcttt ccatgatact ttttgctaat ttctttgacc agttttccat
     9541 tatttgctct cctttaaaaa ttaaaatagg atttgggaca aataccaaga agtatttgtc
     9601 ccaatcgtct atattactgt gccgctgctt tgtatgcttc ctcgtactct tgtgtgattc
     9661 gatcgcctcc ttgtgctttc cagtcttcta cagctttttt gaatccatct tcgtcaagct
     9721 gtcccatgat gtattgcacc gtagcatcaa tgacgatttt ttcaatttct gatcctactt
     9781 cattgttcgt cggtgaatcc aatggcacag aaggatccat tacagcaaat tcttcatttt
     9841 ctttgatcaa ttgattagag aattcttttt cagggtctgt atctttgaaa gagaacgtca
     9901 cttcactagg acgagagctt gataacggtt gcacctcttg ttgccaaaga tccatatcga
     9961 taaattcgat agaaccatca tcattcaatt cataatgcgt tccttcgata ccgccagtca
    10021 tcaatttata cacatcttct tctaaaagat catcaacaaa ttgaagtaat ttcttcaatt
    10081 gtgcttcgtc ttttacttga gatttgggaa atgctagcaa gccgccaata ccatttcctt
    10141 ctgaccaaac atgataaccg ccgccttcac catctgagat cttgtttaca ggcactaatt
    10201 ccatatcatc ttgaatacct tgacccatcg tcttcagatt cttgatatcc accattccag
    10261 tgtagattcc agttcttcct tgtgcaaatt gttcttgctg atcagtcttt tgggtaactg
    10321 caaagtcttg tgccaaatag ccattctcaa atagatctct tgaaaaattc aaagctttca
    10381 tatactcttc tgtatcaaat tctggagtga atttgccatt atcgtctaca gcccagccat
    10441 ttggtgctcc aaaataagaa gtgaaaagtt taaagctggt ataacgaata tcactaggcg
    10501 cacgatcgcc aaaaccaact gtgtcgtcca caccatttcc atcaggatcg ttttctgtaa
    10561 attgtttagc tacttcataa agctcatcca tggtagttgg aacatctagt cctagattat
    10621 ccaaccagtc ttttcgaatg actaagccag cacgagcgta gtctttttgt atcggtacgc
    10681 cgtacaaggc accgtcgata ctcgcagctt cgattctatc ttcagaaatt tgacttaaat
    10741 ttttatagtc ttttaaatat tctgatacat cccagaatac gcctgatttt aacgcattgc
    10801 ggacagaaga gtttttcaac atcgcaattt gaagtgtaac gatatctcct agttcatcag
    10861 aagccaatgc agttgttaat ctttcttctt ttgaagcatc aggaatccag ttgaatgtca
    10921 gttccgtatc ggttttttct tcgatcaagt tcacaatcgt gtctgtaggc ggtgaagcat
    10981 gatgcaaaat atttaaccaa gtgattttag cttttccatc ttctgtctct gaagaactgt
    11041 tacttgaact gctgccgcat gcggcaagtg ctagaattcc tgcaaaacta agacctaatt
    11101 ttacgagcgt tgcttttttc ataaattttc tccttcactg ttttaataat atttttgttt
    11161 tcttctgttt tttctgtata tgagttcttc cattccaaat ggcaaaatat agaatttgct
    11221 gtccacgtga ctgcggaaaa gaagagactg ccaccgataa aggttaaaac acctggcatt
    11281 gtttggatga tcatcagata aatccctacc atacttaata tcaacactaa tgagtaatga
    11341 agatgtgaga atccgaataa aaatgccatt ttcattttga aaaaatatcc tttccaatca
    11401 taatgagcca tgattgggaa aatatagagt gctgagagca catatatgaa cgcagcaata
    11461 tacaaagcga tcctaagata ccaattgtgt atgaaagaaa gatccacaag taaaacaatc
    11521 ccgatggcgc catatatcca gctcatcagc aaggtttcac gatagttttc tttaaaatat
    11581 gtgaagtacg ttttcgtcaa aggaaacgtt tcttttgagc gcagccattt tctaatcact
    11641 gcaaccatcg catacgatgc aggaccgaaa gtaaaaactc ccaaacctgc gatgactaaa
    11701 ccgccccaca gaaaattcaa ataaaccaac tgtaaaatac gatttagcct tccgtttatt
    11761 ttatctaatg actgcagcat atctttccct ctctttctat cttagtaaac gccgtcttct
    11821 cctagctttt tggcaatctt attagctaaa attaccataa acaatcctac gaccccttta
    11881 aacaatccca ttgctgtact gaaacttaac tgtccattct tcaatccagc tgtataaata
    11941 taggtgtcga aaatttctgc aacgcttcga ttcaatgaat tcaataacaa gtacatatgc
    12001 tcgaacccta agtctaatgt ctgaccgatt tttagaatca gcaaggtaat gattactgga
    12061 cgaattgccg gcaatgtaac atgccatgtt ttgcgtaagc gtcctgctcc atccatttct
    12121 gcagcttcat acaactgtgt atcgactgca gttatcgctg aaaggtagat gatcgtagac
    12181 cagcccaact ctttccagat cacttgtccg atatatactg tgcgtgtcca ttcaggggat
    12241 gttagaaaac tgattttttc aaatcccatt gttgcaagga attcattcaa tacccctccg
    12301 tcaacattca aaaatacata agtaatcgaa acaatgatta cccaagacat gaagtgaggg
    12361 atgtaaatga tcgtttgaat cccgtttttc aatcgtttgt tcgttacttc atttaatagt
    12421 aaagacaaaa tgattggcat cggaaaaaac acgaagatat ttaatccgaa caagatcaac
    12481 gtatttctta ataatgtaaa gaatgtcggt tctgtaaaca aacgaatgaa atgtttgaaa
    12541 ccgacccaag gacttcccat tattcctaaa aatggttgat aatcttgaaa agcaataacg
    12601 agtcccccca taggaagata cctaaaaatc aagaaataaa ggactcccgg cagaatcatc
    12661 acatataaaa atttattcgt tttgattcct gcaattattt tttttcgtct tctgagcttt
    12721 tcttcttttt tcgatagcgt cagttgaaat ggttcttgtt tctttacact ttccataagc
    12781 ccctccttag ttttgtgaat gtttgctgct tatgacttca tgatagataa ctatctgaat
    12841 tacgtatata atcattttta ccgtaagtaa aaacgcttac atagcaatga tttttgtgct
    12901 atacaaaacg ataaagtgta ttttttcaca aataaactac gcatagcaaa ccttctattt
    12961 ttttctatct gacacttagc ataggaaatg attatatacg taatctttca ccgatgataa
    13021 attgaacata tagaaacaga aacatttaat ttattttgag gaggagccta tctatatgaa
    13081 aaagaaagtc tctataggag aaagaagttt tacacttttc aacaatacgt ttctaatttt
    13141 gctagctttg atctgtatcg ttcctttttt aaatatcatc gctacatctt ttgcttctac
    13201 acaagaagtc gtcgcgaaaa agtttatcct ttttcctact accttttctt tagacgcgta
    13261 tcgctacatt ctttctacgc cgaccatttt tagagcactt gctgtttcga ttggtgttac
    13321 aggtgtcgga acgatcgtca gtatgtgtgc aacttcactg atggcctacg gtctttccag
    13381 aaagtatttg tttggtcgag gtttcgtcaa ctttcttgtt gtattttcta tgttgttcag
    13441 cggggggatg attccaacgt tcttagttgt tcgttctcta gggctggtca attcttactg
    13501 ggccatgatc ctgcctgtgg ctgttaatgc aatgaatatg atcattatga gaaacttttt
    13561 ccaagccttg ccggatagtt tggaagaatc agcaaaaatg gatggttgca cagattttgg
    13621 tgtatttttc aaaattatgc tgccattagc gttaccgtct attgctacta tctcgctttt
    13681 ttatgctgtt acttactgga acacttatat gactgctatt ttgtatatca acgattcatc
    13741 taaatggccg atacagattc ttttacgcca aatcgtcatt gtatcaagtg ggatgcaagc
    13801 agaaagtagt gccgttgatg tgattccacc agcacaaaca gttaaaatgg ctgttatcgt
    13861 tattgcaaca gtgcctatgc tgattgttta tccttttgtt cagaaatatt ttgtcaaagg
    13921 agcactcgta ggttctgtca aagggtgatt atgtactgaa cgcaaaatgg tagttgtgtc
    13981 agaagtggtc agcttcaaga aataagaagg catttacgaa aattgcttct taaatttttg
    14041 tgaaatttcg gcttattttt cgaagaatct actcctggaa caccgtgtat cagaggttgt
    14101 gaaagagacg ttcagccttg agcgatgata aaaactctca caaacattgc gacgagtaac
    14161 ccacaggagc aataagaagc gattttgaaa aatagcttcc catatttcac aaaattatgc
    14221 cttattgtcg atagcctgtc tcttgaacgc catttattcg taaaaaagaa ctgtgacaag
    14281 acttttgtca cagttctttt tgcgtgctta gccttgaaat ttaactatcc ataaatacaa
    14341 atactagggt tattctaact tcttttattg cttgaatcag tccacaagaa aaacagcctc
    14401 ttgattcaca aatatttttt tcgatattct cccggcgtaa tcgattcctt ctttttaaag
    14461 aaacgaataa aattttgcgg atttcgatat tgcaatcttt ctgcaatctc ttttacggag
    14521 aggttagtct ctttcaacca tttttttgct acctctaaac gatgattttg aacaaaatca
    14581 ccaaattttt ctcctgtttc ctttttaaat acattactta aatagttagg gttataatgc
    14641 aacctatcag cgatcacatc taaagacaga tcacgatcat attctgtgta gacgatatgc
    14701 ataatattct ccgacaaatt tttaaattca tgatcagtga ttgcttgaga gcattctgtg
    14761 attggcagaa ctaattgttc taaaacaagt ttttctagtt ctttgggatg ataattgttc
    14821 agtgctttta cataaatttg tttcatgtta tcaaagtttt ttgaatctac tcccaataac
    14881 tgtcctaatt gaacgagttc attgatcaag cggaccgaaa ctacttcccg actcaaagga
    14941 tttttatttt gtgcaaacaa tgcatcaatc aattcatgta ctaaattctt cacttcctga
    15001 tttccagaac gaatggcttc aaacaatcga ttttgttctt ctatcggata tctaataagt
    15061 gttttttcat gagcagcagg aacgatttcc tgataaaaaa taattgaact tggaccagta
    15121 tttacacgat aatacagggc ttcttttgcc cgatcaaccg attgcttgct ttcattcagc
    15181 gtttcgaacc ggttactgat gcctatactg atagttaaat tcaagtattc ttttacacgt
    15241 ttttgaatga gtcggcaata ctccatgact tttttgcttg cttctgcatc atctttatcc
    15301 attcgataaa tagtggcttg catttcttca ttcaaaacga tcggcagcat tcgattttcc
    15361 tttggcacaa tttccgtgat catgttgttg atcgctaaaa gtagcaaatc acgctcccca
    15421 ccgtgactat cttctaacag atcgatttga accaaagctg tataatagca gtcattttga
    15481 gaactataac caaattgctg caaccgctga tctagttctc gcctttctac tcgatttcta
    15541 aataaactaa gtacaaatag cgtttccagc tgtggctttt gggtaactaa gtgtgcactt
    15601 aacgcttctt tttctcctat gatccgactg actgattgag cgaccaaatc tagttctttc
    15661 ccactgaatc cttttgattt aagattcaga cgttcctgta tttgagaaat cggacgggca
    15721 aatctgtccg aaaataagta ggaaagaaga ccaatgagtc ctaccagtgt taaagacact
    15781 gcaactagcc cgattctcag attgcggatc attgcgccaa ttgcactaga gtcaacttct
    15841 aatctgtaaa tccaattatt ataatctgat ttcagtacta tatacgtttt atctttttct
    15901 cttatcactt tcagctgatt gtaatctaga tccacgtccg ttaatacagt tggttgctct
    15961 ttatttttta ctgtctgagt agtaaaaagg ttgccaactt cgttttcaat agaaagtatg
    16021 tcttctccac tttcaccaac aacttgttct attgtatgtt tctctatcgt agcaattccc
    16081 aatgcatatt tttctttttc aaacattggc aaagaaatca ccatttcgat tcctttgttt
    16141 ttcttttgcc aaaataaatt atttttgtta tttatgtact tctgttgata cttttcaatt
    16201 tcctcttcgg tcagttgact caacgatcca ttgattactc cccatttacc agccaaactg
    16261 actaaatcat aattattatc atccataaca atcgtttcta taaaatttag ttctttcctg
    16321 atttcagaat aagtggagta atctgtatat gtcagcggat gcttgaatat gtccgagaaa
    16381 tttttggtcg aaccatacgt agaaaacgaa tattcaatcg ttctaatttt ccattcaacg
    16441 ttagttttaa cttgcctgag caaattcttt ttgtctgtta aataactcga ttcgatcgat
    16501 tcataggtat tctggtaaat gaataaacta aatcctatga caagtacggc tcctaataaa
    16561 aacatcggta aaaatatttt ataaaatact gtacttttct tcattttctt cctctcctcc
    16621 ttttaaaata aaaggctttc atttttctgt aaaattgata ctcatctaat taaatgagca
    16681 ttccccgctt tcaagaatag cataattaaa aataaaaatc caagtaattt ttaacgtgtg
    16741 caatcttttc atatcaaaga tctattgact tatcgtagtt atcgtaatat gatgaatgcg
    16801 ttaacaaaaa gtgaaaaagt aaggaaaatg atggaatggt aacgatcaaa gatgtcgcta
    16861 aagcagctgg cgtttctcat acgacagttt ctcgtgcttt gaataacagc agtttaataa
    16921 aaaatgaaac aaaacaaaaa atacgacaac tagctaagga aatgaattat tcgccaaact
    16981 atagtgcaaa aggtttagtg aatcaaaaaa aatatctaat cggactattt ttctcaagtt
    17041 tgcataaagg aacttcatca aatttcctag cagattccat tgcaggcatc cattcagtat
    17101 tagacacaaa ttacagttta tctgttgaaa gtatcgatgt tgttgattta acagaaatca
    17161 acctgcaacg ctacgatgga ctgatcgtta tgagtcaaag tgatgatgat cagcctttca
    17221 tcgactatct caaagaaaaa gaatttcctt tcgttgttgt caatcgtcac atcaatgatc
    17281 cagcggttat caacgtcact gccgatgatg cggccggtgt tatgcaggca gtagattacg
    17341 gtattggatg cggacatacg aagatcgctt atattggcgg aaagaaaaat tttcgttcga
    17401 caaaagaacg ggaaaaaggg gtattgaaaa gtctaaaaaa agcgagcatc cctgtcaatc
    17461 cagcctattt tttaacagga gattacagct tagaaagcgg cctccaaaac atggaatacc
    17521 tattatcact ttctgacctg cctactctgg ttttttgcgg caacgacgac atagcgattg
    17581 gtgcattgag agctgtacat ttagctggcc tccgtgttcc ccagaacatt tctctgatcg
    17641 ggttcgatga ttctccagtc gtctcctatc tcaatccgcc gcttaccacc gtacgtaagc
    17701 ctttgaaaga aatgtgtcaa gaaggaacaa agcttttact agatttgatc gaaggcagga
    17761 cgatcgaaca atctaaaatt gagctctcca ccttgttgat ccttcgtgag tcgatcaatt
    17821 acttgtaaac tacaagtaat tttatataaa aatgttaacg tgtgcataac acagaaagga
    17881 agtttatatg aaaaaactga caaaagaatt agtgaaaaaa aacgaagcac ctgtaaaagt
    17941 tcttcagttt ggtgaaggca attttatgcg tggatttatt gattggcaga ttcagcagct
    18001 gaataaccaa caattgttta aaggtaatgt tgcaatcatt caacctttag aacaaggtct
    18061 tggaaatatg atgaaccagc aagataattt atatacggtc attttacaag gactcagcaa
    18121 tggcgaaatc gttgatactt ccgagattat caccgtagta gaacaaatga tcaatcctta
    18181 tgatgaatgg gatgcttatc tatctctagc agaaaatgac gacttggcct atatcgtctc
    18241 caatacaaca gaggcaggaa tccaattcgt ggagactgat aatttaacag atactccccc
    18301 acagagcctc ccgggcaaac tgacagcctt tttataccga cgtttcaaat taaacaaagc
    18361 tggctttacc atcattcctt gcgaactgat tgacagaaac ggcgaacgtc taaaagaaat
    18421 cattttacgc tacgcagaaa aatgggattt agaacctgct tttgttgagt ggttagaaaa
    18481 agaaaatgtt ttctgttgta gtttagttga tcgcatcgtc ccaggttatc ctcgcgaaga
    18541 tgcaggtgtt ttcaatcaac ggcataacta tgaagatcaa ttgatggtca aagcagaacc
    18601 attcatgctt tgggtgatcg aagggcctaa agaattggag aatcaactac cattcaaaca
    18661 ggctggcttg aatgtcatca tcacagatga tatgactcct tatcgtgaac gtaaggtgca
    18721 tttgcttaat ggaccgcata cagcgatggt ccctcttgct ttgttggcgg aattggaaac
    18781 tgtagaagaa gtaatgaaag atcctttatt ctccgcatat gttgatcagt tattcaatct
    18841 agaattgatt cctatgcttt ctttacctaa agatgaatta gccatttatg ctgaccaaat
    18901 caaagaacgt ttcttgaatc cttttgccca tcacaaatta gaagcgattt cattaaatag
    18961 tgtttcaaag ttcagcaccc gtctgcttcc agtgttcaag aagtatatcg aagaacaaaa
    19021 tcaggtacca ccgttaatca ctgtttcact agctgcgcta cttcttatgt atcgaggcga
    19081 tcaagtaaag ccacatgatg atgaaaagac gataagcgaa tttactgatg cttggtctga
    19141 taatggaaca gctattcctc gattattgca aaacgctgcg ctttggggag aagatttgtc
    19201 tcaaataccg aatgtgacag atacagttca agaaatagct gcaaagatcg atcaaaaagg
    19261 tgtccggtca gtattacaag aaatgaaagg aacgatggtc catgcttgaa accctgcaac
    19321 caacaatgaa attaaatcga cgagacagtg tggtcgtcgc attaacccct attccaaaac
    19381 aaacacaact gacgattgat cagcaaacga tcaccactct tgaagatatc ccgcaaggtc
    19441 ataagattgc attgggtgat ttgaaagctg gagacaacgt gatcaaatat ggctatccaa
    19501 tcggtcatgt gacgacagac gtaactgccg gtcaatggct gcacactcat aatgtaaaaa
    19561 ccaatctttc tggtgaattg gattatcgat atgaaaaaga tgtccatcca agtcattacc
    19621 tttttgagaa ccgaacattt caaggttact tgcgaaaaaa tggaaaagta gggattcgta
    19681 atgatttgtt tttagtacct accgttgggt gtgtgaatgg cattgctgaa ctgatcgtca
    19741 aacagttcaa ggaaaaacat ccagatctgg gacagtttga ccatattacg attttaaagc
    19801 atccttatgg ctgttcgcag cttggaaaag atcatcaaaa tactcgagaa attttagcgg
    19861 acgcagtaaa tcatcccaat gccggaggag ttttagtatt tggattaggt tgtgagaaca
    19921 atacagttcc tgaattcaaa aaaattttag gtgaatatga tgaagaacga gtgaaatttc
    19981 tagtggctca agatgtttac gatgagatcg aacaaggtgt ggctctatta gaagagcttt
    20041 taacagcggc ggaaaatgac cagcgcatat cagtcccgct ctctaagttg aatgtcggtt
    20101 tgaaatgcgg cggttctgat ggtctatcgg gaataactgc taatccttta cttggtgcat
    20161 tttctgacta cttgattgct caaggaggca gcacgatctt aacagaagtg ccagaaatgt
    20221 ttggcgcaga acaagtattg atggcacgag cggaaaatga agaagttttt gactcaatcg
    20281 ttgacttgat caatgacttc aaacagtatt tcttgtctta cggggaacca gtttacgaaa
    20341 acccatctcc tggtaataaa gcaggtggga tcactacatt agaagataag tctttaggct
    20401 gcacgcaaaa atcaggaagc tctcctgtcg ttgatgtgct acaatatggc gaaaaaatca
    20461 gaaaaccagg gcttagttta ttgcaagctc ccgggaatga tcttgtagct gcctctgctc
    20521 ttgcttcatc cgattgccag ttggtcttgt ttaccacagg aagagggact ccgtttggct
    20581 catatgtccc aacggtcaag gtatctacga atacaactct ttttgaacgc aaaggtcatt
    20641 ggatggattt taatgcaggc gtcttattag aacaaccaat ggaagtaatc ttagaatcgt
    20701 ttgttcaaaa aatcatcgct gtagctagtg gagaagaaac gaaaaatgaa caaaatgacg
    20761 ttcgagaaat tgccattttt aaaaacggag taacactttg attttcataa aacagtataa
    20821 aaagaaagaa ggaagattta tgtttttatc tgatgatttt ttattaaaag acgattgggc
    20881 gaaaaagctt taccattcct acgcaaaaaa catgcccatt attgattatc actgtcactt
    20941 agatccaaaa gaaatttacg aaaacaacaa tttcagcaat ctgacagaag cttggttagg
    21001 aggcgatcat tacaaatggc gcttgatgcg agcttgcggc gtgcctgaag aaaaaatcac
    21061 aggaaatgct tctgattttg aaaaattttt agcttggtgt caaaccgtac caaaaattat
    21121 cggaaatccg ctttacagtt ggtcacatct tgaattgaag cgtttcttcg gtattgactt
    21181 gttgataaat gaagaaaatg cagagaaaat ttggacacaa gcaaatgaaa ttttggcagc
    21241 tcctgatttt cgccgtcggg aaatcgcaaa aaaatcgaat gtagaagtga tttgtacaac
    21301 agatgatccc gcagacgact tgcactatca tcagttgctt gctcatgaag aaccagattt
    21361 gaaggttcta ccttcattcc gtccagataa agcactcaat attgaaaaag aaggatttaa
    21421 tgcatgggtc acactactgg aagaagcagc tgaccaaacg atcaccacct atcaagaact
    21481 gatagaagtc ttagggcagc ggatagaata cttccatcaa catggatgtc gtgtttccga
    21541 ccacgcttta gacatcttac gctataaaga agcggatgaa acagtattag aagagatttt
    21601 tcaaaaagca cgagctaaca aactattatc ttcagatgaa atcgatgctt atcgtacaga
    21661 aacactgata catttgaccc acttctatca ttctcataat tggacgatgc agctgcacat
    21721 ccatgcttat cgtaactgta atacacaaat gttggaaaaa ttagggcccg ataccggcta
    21781 tgatggaatg aatgatcttt ctttgactat tcctctgcaa aaattattga accgtgcaga
    21841 agaaacggat cagttgccaa agacgatcct ttattcattg aatccgaatg actatcctgc
    21901 gattttagct ttgatgggct gtttccaaaa agaacagaat ggaaaactgc agttaggttc
    21961 tggatggtgg tacaacgata ctcgagctgg tatgcgcgat caattgacac aatttgctga
    22021 tggtagtgct ttggggaatt ttgtcggcat gctgacagat tcaagaagtt tcctgtccta
    22081 cactagacat gaatatttcc gtcgtgttct ttgtgaattt ttaggcgaaa tggtcatgcg
    22141 aggagaagca cctgaagatg aacaactttt aggaagtcta gttcaagcga ttagttatac
    22201 aaatgctaaa aattactttg gcttttttgc ctaattgatt aatagtaaca gtaacgaatc
    22261 aaaaacagga tagtgaaata acggaatccg tttatccacg ttttagagaa gaagacggac
    22321 caaatttgct ccgtcttctt ctcttgctgt tctgcttcat tgcctataaa cctttatgaa
    22381 agaagagata acatgcctac tatccatatt gccggtgatt ctactgcggc agaaaaagat
    22441 gaaaacaagc gtcccgagac aggttgggga gaaaaaatct ctgcttattt ttcacccgag
    22501 atccagatca ataaccaagc aaagaatgga cgcagcacca aatcatttat ccaggaagga
    22561 aggttagccc gtctagcttc acagtttcag ccgaatgatt tcttatttgt acaatttgga
    22621 cataacgacc agaaaatcca agaagaaaga ggtacgcttc ccttcggtag ctatttaaaa
    22681 aatctgcaga tattttttga atgcgcaaaa aaagcgaacg tccaaatggt cttactgaca
    22741 tctgtgacgc gacgagatta cttagaaaac ggaacattga atccagatat acttggagac
    22801 tatccaaaag cgatgcgtgc atttgctgag aaacaccata ttcctctttt agatgtgttc
    22861 tcaagatcac aagaattgtt ccagaccttc tcaaaagaag agacaaaaaa attctacctg
    22921 catcttatac ctgatacttg taaaaattac ccagaaggac taaaagacaa tacccacttt
    22981 agtccagaag gcgctgataa agttgcccga ataattgttg aattgataaa agaatcccgt
    23041 ctgcctcttg ctaattattt acaaaaagga gtttgagatg tttccattaa atttaggcat
    23101 ccgtgcccac gatttagatg ttcgaaatcg tgaagaaatc gttcaaaaag tatcacagct
    23161 tcacttgtcc catatccaat ttgcaccaca aaaagcattt cctgaacaac ttaaattaac
    23221 tgacatgaca cttgggagtg ctgcctattt tggtgaatac ttccaaaaaa acaatatcga
    23281 actttccatt ttaggatgct atatcaacct atccagcaaa aatcaagaag ttcgtgcaca
    23341 agcattggca actttcaaac agcaaattgc tctgtgctct gcctatcaag ccaagatgat
    23401 tgctacggaa accggtagtg tctctgtcgg ttacacaaaa gaaaatttta ctgaagaagc
    23461 ttatcagatc gccagaaatt ctatcattga attagtcgct catgcagaaa attttggcgt
    23521 aactgttgct gttgaagcag ggatcaatca tccaatctat aactatcagt tagctaaacg
    23581 tttaatcgat gaagtagctt caccaaattt aaaaatcatt ttagattgtg cgaatttgat
    23641 gcataaagaa aatcatgctg atcaggaaaa agtgatccga ggtgccttgg acaatttaca
    23701 tggttatatt acagcgcttc atctcaaaga ctatatcatg gaaaatggaa agatccgcat
    23761 ggtacctgtt ggcaagggct gtttagattt ccgcccggta ctgcattata tcaaatatga
    23821 gcgtcctcta atgtacgcca ctcttgaagc tactcctgag atttatgttc ctgaagcaat
    23881 agaacacttg cagtttattt accaagattg ttaaggagaa gattatggag gaacgaatac
    23941 agaaaagtta tcaaaaatat catgcaaatg cttttcagac attgctgcat ctttatcagg
    24001 gaaattattg gcgttttgcc atgtcaggac tcttttttat tatcaaacat cttccatctt
    24061 ggttaatgcc gatcgctatt gccaatgtaa tcaatgcagc ttcatcgaaa gattcttctc
    24121 agttgcaggc catttatttc aacgcttttt tgatggcttg tctgattggt caaaatatat
    24181 tgacgaatta ttttcatgta aaataccata gcacttcgat ccgtcaagta gaagctggac
    24241 taagagcgac ccttatcaag aaaatccaag aactatctat tccttatcaa aaagaattgc
    24301 aatctggacg aatccagtca aagatcatcc gtgatgtaga agctatccaa acgttgtcct
    24361 ctcaagtatt tgtcagcgga ttaaatatcg ctgtcaattt attcgtagct ttaggtatca
    24421 cgctttataa aagtcctgtt gttttcatgt ttttcttatt gactgttcct ctcgccgcat
    24481 tcgttactct ctttttccgt aaaaggatca gacagtccaa ctcagacttc aggcaagaaa
    24541 tggaagagac ttctgcaaaa gtgatcgaag tgatcgaatt agcaccgatt gctcgagctc
    24601 atgggttaga aaaacacgaa acaactcgct taaataagca ttttactcaa atctatgaaa
    24661 aaggattcca cttagatatc ctgcaatctg ttttcggatc agtcagctgg gctagttttc
    24721 agatatttca gctgatttgt ttgattttca ctgggatatt agctttacaa gggcgcatcc
    24781 aacccggcga tgtcgtgatg tatcagacgt atttcacgac gatcgtcaat tccgtttctg
    24841 gaatgatcac gctgattcct acgatttcaa aaggcatgga atctatcact tctattggcg
    24901 aactgttgat tgctggcgat gtagaaactt atcacggtaa gaaaaaaatc caacatttgg
    24961 atggaaattt tcgttttgag gatgtttctt ttctttatcc ggactcactc aaaccgatta
    25021 tcgatcattt ttctctagag gtgaaagctg gagaaacaat tgcttttgtt gggccatctg
    25081 gctctggaaa atctacactc cttaacttat tgattggatt tatccagccg acttcaggaa
    25141 aaatttttct agatgatcag cctttggatg aactggatat gcgaagtgtg cgaaattatt
    25201 tagccgttgt tcctcaaacc actttgttgt tctccgcttc gatcaaagag aacatcactt
    25261 atggcttgaa aaacgtatca aaagaacgtt tagatgaagt catcgaagct gcgcaacttt
    25321 cttcactgat tgaccagctt cctgatggtc tggatacctt agtaggcgaa catggcaaca
    25381 agctgtctgg tggccaaaag caacgtatct ccattgcacg agcgttgata cgtcagccaa
    25441 aaatcatttt gctagatgaa gcaacctcag ctttagataa ccagtcagag aaaaaaatcc
    25501 agcaagccct gaattacctg acagaaacac ccactacttt tattgtggct catcggcttt
    25561 caacgatcaa agaggcagat aaaatcgttg tgatcgatga aggaaaaatc gttgaaatag
    25621 gtacttacga aaaactgtta gctcaaaaag gctatttcta ccgcttgaaa aatgaatgac
    25681 aaaaaatagg agctatgaca agacttttgt cgtagctcct atttctgaat aaacggtgtt
    25741 ctcctgccaa ctcctcggtc acaagtcaca atagtcatca aacatgagaa gctgtttttt
    25801 gaaattgttc cttgtaactc agagttaaac ggtaggctca cgacctcttg gtaaatggtg
    25861 ttcaagagac agcctatcgg caataagaag caattttgtc atcgctcaag gccgaacgtc
    25921 tctttcacac cctctgttgt ttcataaaat agttttattc gctgtaaagg tcgcgaactg
    25981 gtgtcatgat gatacggttc aagtcattga tgattgtact gaagccttgc tctttttgca
    26041 tcaagtcatt gattacttct tctgattgga ttttcacagc catttcttgt gctttcattg
    26101 catcttcgtc gctgaactct tgtccactca tttgtttttc ttgtaattcg atttgcatcg
    26161 cttggaagtc tttgaacagt ttgtgtgctt cttcgtttgc ttgtactttc tcgtacgctt
    26221 ctttcaacgc tttgaattct ggtaattcac ggatctcacg ttcgatttga ttcgcgctat
    26281 cgtagatgtt tgacatgtta tgccctcttt tcttttagta gttactctct attgtaacat
    26341 ggggttattc gttttcaaag gggctactct ccttgatatt tccgccagag gtctttagcc
    26401 gcttcaccaa ttccctgaac gccttctttc accatatcgc cgaagttttt cagcccttct
    26461 tttgcttgtt caccatattg attcaagtta tcttgccaat ttccagactc tccattagaa
    26521 tttcctgtgt ttccaacttg atctgcagct accacttgtc ctcctgtggc ataggcatct
    26581 gctacactaa atcctgtttt tgaagtatac ggaaggatgc cttctgccgc tgatttgaag
    26641 actttcccga caacatttcc acttgtccct tccaaatagt gcagcttgct ggattcttcg
    26701 taacccatcc atgtcgaaat cactacgtct ggcgtgtaac cgatcatcca ttggtcattt
    26761 gcttttgtgg catcgaaatt agtttctgtg gttcctgttt tccctgctat cgtataacct
    26821 gcaggattcg cagcagccgc tgttccattt gaaaatgtac caagaagcat gctggtcatt
    26881 tcatcggctg tttcctttga tatgacctgt tttggttttt tgtttgtgtt atcgacgatc
    26941 accgctccag tcgaatcaac gattttcgtg ataagatgcg gcgtatacat ctgaccatca
    27001 ttggcaaatg cactgtaagc acccgccatg atcaatggag atactccttt ttccagtcca
    27061 ccaagggcta agccataata cttatcagat tttgctaaag gcaggccgaa ttcttctgct
    27121 ttatcatatc ctttttgtaa accgatctca tgcaaaagcc atacagtagg caaattcaaa
    27181 ctttgtgcaa cagcttgata catcggtact tctccttggt atgtcccgtc aaaattatgc
    27241 gcatcgtaat aactttgcgg ttcgtctttc aagatactgg aaggcttgta tccagcttcc
    27301 agtgctggtg tatatacgct taatggtttg atcgttgatc ctggtgaacg acgcatctgt
    27361 gtagcaaagc tgaatcctct aaagacatga tctcctcttc gtcctactag tgcttgtaca
    27421 ccacctgtat tcggatctaa agctactgaa gcagattgga ccatggcacc atctgaagca
    27481 tttgctggga ataatgcact gttttgataa gtagcttcta attgcttttg ataatcctga
    27541 tctaaagctg tatagatttt atagccttta ttcatgacat cttcttcatc aagaccatac
    27601 tcgttaactg cttcatcgat cactgcatca aaataataag gataacgata accagaatct
    27661 gaattatcgt atgtgtcatt aagatacgcg ctgatatctg tctggctttc tgcttgttct
    27721 tgttcttttg tgatttttcc gttttcttcc attacactta gtactgtgtt tttccgatca
    27781 ttcgcatttt ttgggtgatc gatcggatta taaataccag gacctttcag cattcctgcc
    27841 aaagtagcag cctcagacag cgtaacattc gttgcatcta ctccaaaata ctttctggaa
    27901 gcgtcttgga caccccatac cccgttgcca aaataggcat tgttaagata catggtcaag
    27961 atttcttctt tgctgtactt cttttcgatc tcgattgcca aaaataattc cttcgctttt
    28021 cgatcaaacg tttggtctaa tgtcagatac gcatttttgg caagctgctg agtgatcgta
    28081 cttcctccgc cccctgatgt tccgccagac aagaccattc ggactgctgc acgggcaatc
    28141 cctttgatat caaacccgtg atgctcgtaa aaattcctat cttctgtcga gattactgca
    28201 tcttggatat agggagaaat cgcgtttaat tctacatatg ttcctttttg ggcgtacagt
    28261 gtgccagctt cttcgttttt cttatcataa atgactgtcg attggcttaa tcctgatttt
    28321 aacgtttcga catttgcttg ttttgctaaa ataaagaggt agatacttgt cactaagaca
    28381 cagatcaaac caatcagcaa aatcaatttg ttcacatgat attttttcca gatgcgcttt
    28441 cgccatcgat ggaattgtcc gagatatggt ctgatccaac gccaaaaact gatcaaagca
    28501 gcttttatct tttctaaaat gctttgaaaa tccatttgct tcttctcctg tctatttgat
    28561 tcaatcgtct tattttacca tagtgaaaga gtgttttctt cctgcttaag ttgtagttca
    28621 atatgttttt ttacgttagt gcaagtagag taacataaga atgaacagaa aaatgtaagt
    28681 tttttcttaa atatgtttta aaaatggtac aattagtctg cgattgcact gaaaggagta
    28741 cacttgtgaa aatcacgatc actttaccta tgaaccatcc tgaaacaaca attcgcgact
    28801 tgcttgaaaa ggaatggtta gttcctagaa aggttcgaca ctttttgcgt atacgtaaaa
    28861 acgtttggat caatcaacaa cctgctttgt tccatcatga agtaaagggc aatgaccaga
    28921 tcacattgtt attcgaagaa acagattata attatcaaga agttcaatta ggagatgcga
    28981 agcacatccg tgttttatat gaagatgagc atttgattat agtggataag cctgcaggaa
    29041 tgaagactca tccaaatgaa ccgacagaaa acagtacctt actcaatcat ttagctgctt
    29101 atctcgacac taaacagcaa cgaccttacg ttgtccaccg cttggacaaa gaaacaagcg
    29161 gtgccgtttt atttgcaaaa aatccatttg tcttgccgat acttggaaga atgttggaac
    29221 aaaaaatgat ctatcggaga tatcaagcca tcgtatgggg agaaattgcc aaagatatga
    29281 ccatcaccaa taaaattgga cgagatcgcc atgatcggag aaaaagggtc gtagatgata
    29341 aaaatggtca gagcgccttg acacatgtca ctgtcaacaa agtactttct gaacaaacac
    29401 aagtctattg tgttttggaa accggacgta cacaccagat cagagttcat ttagctcata
    29461 agggacatcc gatcgttgga gaccctctct accaaaatag aacagctgaa cgactgatgc
    29521 tccatgcatt agaactgcac atgatccatc catttactca agaaaaaata gtcggtaaag
    29581 ctttacctgg attatgggaa tagaaataaa aaagaacaga gtcttatcag cagataatga
    29641 taagactctg ttctttttgt gtaatttgtc tgcttacttt tgatgttcgt gttctgctaa
    29701 cagcaaacta ccgataatcc cactttcatt cggcagtccc caacgaacga tatattcact
    29761 tacttgaggt gtttccatat aaccacccat caattcaaca aattgctgac ggattttttg
    29821 aaggagatga tcctgattca ttaccccacc gcctaaaata atcttttcag gtgctaacgt
    29881 caacgtatag ttgatcagtg cttgagcaat ataataagcc tgaatagccc aaacaggatg
    29941 atcttgcggc agatcttgcc ctttgatccc tgtgcgtgcc tcaatagacg gtcctgctgc
    30001 taatccttct aaacaatctt tatgataagg acaagtacct tcataatggt catctggatg
    30061 gcgtttcacc caaatatgtc ccatttcagg atgtgcgatc cctgaaaaaa tttcttcatt
    30121 gatcacgaca cctgcaccga ttcctgttcc cacggtaagg taaatacaac tattttttcc
    30181 ttgtgctgct ccttgcttca attccccata tgcagcagca ttgacatccg ttgtccaagc
    30241 agcaggaata ttgtagcgtt ccttgattgt gccaagaaaa tcgaattgtg cccacccttt
    30301 tttgggagtt gccaaaacgt atccgtaatt atcttttgtt ttgtcgatac caatcgggcc
    30361 gaatgagcca atcccgattc ccttcaattc ataacgatca aaaaattcaa atacctgatc
    30421 caatgtttct tctggtgttg tggtagggat acttaatttt ttcttaattg tcaaattaag
    30481 ttagacagat catcaatact tacgtaactc aaaaatactt ccaaaggtgt tcgataatcc
    30541 agtgattttc taggaatatt atttcgttta gatgcgacag attgaataaa aaattcatct
    30601 actgaattga aatccataga tttcaataaa ccatctctac gtaacaatcc gttagaattc
    30661 tcgtttaggc ctctttgaga cggcgttcct ggatcagcga aataaatggc aatatcattg
    30721 acattactga tctgtttcca atttgaaaat tcctttccac aatcaaaagt gatggatttg
    30781 aataggtttt tcggtacaga ttcaaaccat tgatttaatt ttttttcaat atcaatcgct
    30841 tgtctaccac acggtttcaa tgtgatgata acttttgata atcgttcaac taaggtaatt
    30901 acagcacttt tatgtttcag acctacgata gtgtcacctt caaggtgacc aaactcattt
    30961 gagaattggc tataatcctt ttcacgttca tgaatagagc ggcggaaagt ttgttttcca
    31021 cgtctttctt gatgtccatt cggtttacgc ttgcctttca tcggtaagtc agaagaatca
    31081 aatagcccct ttttgaacat acgataaatg gtacgagcag aacaactaat aggaaacgct
    31141 gcacgaccaa caatcacatc gggtgtccat ccttgaacaa cctttctttg aatatattct
    31201 gattgttcct cgggtaaaac aagcggacgt ctaccacagt ttgatttgtt tttcttatac
    31261 tgttgataat attctaaggc tgattttcct tgcttgaaga acagatatac tttatggatc
    31321 gtttgtcttg aacgattcaa gcaatgggc
//
LOCUS       DAZKLM010000024        30798 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3764
            SAMEA115706913-rid22582563.denovo.024, whole genome shotgun
            sequence.
ACCESSION   DAZKLM010000024 DAZKLM010000000
VERSION     DAZKLM010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706913
            Sequence Read Archive: ERR13196647
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 30798)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30798)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287823.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287823.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,004
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,849
            CDSs (with protein)               :: 2,849
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 41 of 96
            Pseudo Genes (incomplete)         :: 52 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 15 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30798
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706913-rid22582563.denovo.024"
                     /isolate="VRE3764"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(144..308)
                     /locus_tag="ACG842_001019"
     CDS             complement(144..308)
                     /locus_tag="ACG842_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0247580.1"
                     /translation="MKNLIAGGFLFVGGGILMNTDNFGNVIPFLGAACALIGVGMLIY
                     FVFSDDGKYG"
     gene            603..1931
                     /locus_tag="ACG842_001020"
     CDS             603..1931
                     /locus_tag="ACG842_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HFQ0247581.1"
                     /translation="MKAITPDMTTKFNKQFQANIKQQALQRAVVKNGITASAENVSAH
                     VENTPVFSIDLATGKVANQMQSGRCWMFAALNTFRHKMLNQFHLKEFELSQNYTFFWD
                     KYEKANYFYENIIATGKEAIDSRKVAFLLATPQQDGGQWDMIVSLFQKYGVVPKSVMP
                     ESANSSNSRDLNNYLNKLLRKDAVVLRKMVAQGETLDAIEEKKEEMLESIYNFLAISL
                     GTPPKEFDFEYRDEEKNYHLDRGLTPQIFYDKYIGVKLDDYVSVINAPTKDKPFNRSY
                     TVEMLGNVVGGKEVKYLNVDMETFKKLAIAQLEEGESVWFGCDVGQSSTRTSGIMALD
                     AYSMDDLFDTDFTMTKAERLDFGESLMTHAMVLTGVDLVDGQSTKWKVENSWGEKVGK
                     NGFFVMSDAWMDEYTYQIVVRKDLLTKEQLNAFNEEPIVLSPWDPMGALA"
     gene            2133..2966
                     /locus_tag="ACG842_001021"
     CDS             2133..2966
                     /locus_tag="ACG842_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HFQ0247582.1"
                     /translation="MDIQTVLSDLKLNGKVIPVVGGDVNQTYRIKTEHRAYFLKIHPN
                     VKKGFFEAEVDGLKELSAFVRVPDTYMLGETSEGAYLLMEWIEPGKGDQRDLAAALAN
                     LHQQTAPQFGFRKDNYLGTLVQKNSFEEDWWTFFFKDRLESQISLAEETNRWNVQRQE
                     KYLRFKERVLKSVEPKKITPRLLHGDLWSGNVFFDQQGHPVFVDPAVSYGNREQDIAM
                     SQLFGGFRPEFLDAYQTIFPLEKGWKDRLPIYQLYYLLAHLNMFGESYGSQVDQLLEN
                     F"
     gene            2982..3719
                     /locus_tag="ACG842_001022"
     CDS             2982..3719
                     /locus_tag="ACG842_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP thioesterase domain-containing protein"
                     /protein_id="HFQ0247583.1"
                     /translation="MAKKYTKKHEVSYYECDINQTMTFPSMLGVVIKTSEDQSDQLGR
                     GSEFVNSFGLTWVITNYSMEINRLPRVGEVISVTTQAMEYNKFFCYRNFWIHDEDGKE
                     LVKIESVFVLMDFVNRKMSSVNEEIIAPFESEKIKKIKRQEKIEKIDSGFMLPYRVRF
                     YDIDSNQHVNNAMYFNWIIDVLGYDFLTTHVPEKVIIRFDKEVEYGNEIESHFEQIPI
                     ENGVKTKHEIRMQEQLYCEANIIWKNK"
     gene            complement(3874..4842)
                     /locus_tag="ACG842_001023"
     CDS             complement(3874..4842)
                     /locus_tag="ACG842_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HFQ0247584.1"
                     /translation="MKKILVLHTGGTIAMKENMLTGGVSPDVANPLLDAEITIPKHVE
                     LLVEDIFNLPSPHIMPSHMLVLKERILSAYASGISGVVITHGTDTLEETAFFLDTTIG
                     NKLPIVLTGAMRSSNELGSDGLYNFESAIRVASCEEALDKGVLVVMNDEIHSARYVTK
                     THTTNVATFRTPTLGPIGLVTKNRILFLQELLETKRLDISAVDGTIPIVKAYAGMQGD
                     LLEAIAHTKVDGLVIEALGAGNLPPQALAALEKLLVQKIPVVLVSRCFNGIAEPVYDY
                     EGGGKELEKMGIIFCNSINSQKARLKLLIAVNYGLSGEELISFIQN"
     gene            5074..5907
                     /locus_tag="ACG842_001024"
     CDS             5074..5907
                     /locus_tag="ACG842_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HFQ0247585.1"
                     /translation="MIRIGLTSFKEHDKLTGKKNNTLYEYAGFLPLVELDTAYYGIPR
                     KSSVENWISEVPESFRFVVKVYQGISGQGDWKPYYQTEEEMIAHFLDVMSPMVDAGKM
                     FCFLVQFPSSFKCTKENVQYLRKLRKWFEGYPVSIELRDYSWYSDEFLERTRNFMSRA
                     AFSLAIIDEPQLLNTTIPFDPFVTNPNFALFRFHGRNAQGWQANDKEWRKKRTLYRYS
                     EKELQELAHEVKVVDSKTKEVGVIFNNNSGGDAAENALALQKILNIKPDGLNPTQMDL
                     F"
     gene            5941..6780
                     /locus_tag="ACG842_001025"
     CDS             5941..6780
                     /locus_tag="ACG842_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFQ0247586.1"
                     /translation="MIKAIFFDIDGTLVTNRSKALESTKQAIEYARKNGILCGVATGR
                     SPVKIKEIIDELELDMYVVYNGQLVFTADRTIIDHPFEQKVLEHIVEFADENHRQIVF
                     GARNRLDGSTTMLLGQSIFIKRLVSFLPRKFPVRLMKKILQVFSPHRQKDRYETLEIL
                     KEPIYQCILLSPESEQKKLEHRFPECTFQRSNTYTVDIIPKGGSKLLGIQAFANAVEI
                     EMEEIMAFGDHYNDIEMLKGVGIGVAMGNAQIEVKHAADFVTQSNEEDGIYEGLKHFG
                     LIY"
     gene            6806..7366
                     /locus_tag="ACG842_001026"
     CDS             6806..7366
                     /locus_tag="ACG842_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFQ0247587.1"
                     /translation="MEAETPFQKVETFHKTFDPRRPEIPTAFTENEAHTRAAFKIEEL
                     VEFVAATSPNKEQLHEAVMLLHEALDKAEEKILGKTQWGSDPLVEQVDALTDILYFTY
                     GSFSLLGIVPDRIFEIVHQANMGKLFPDGKPHYDPVTNKVLKPENWQMEYAPEPKIAA
                     EIQRQIQEAIAKKKSADIGMCTNEKE"
     gene            7565..9286
                     /locus_tag="ACG842_001027"
     CDS             7565..9286
                     /locus_tag="ACG842_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HFQ0247588.1"
                     /translation="MGNNVIIGIVIAIIIVAIVVFLVSYLMKKKNQDRLDALEKRKEE
                     LFDLPVIEEVDDVKKMHLVGQSQNTFREWSQKWNDISTSSFAELESQIFEVENLNESF
                     RFLKGKKAVVEAEATMDQMEKQVTEIREGLKELRETEERNSLAVQQALDVYEELKEKV
                     REDEHEYGPALFELQKQIKVIEKEFTQFVTLNTSGDPVEAREVLDKAEKHTYEVEALM
                     KKIPPLYEELHTTFPEQLEEISDTYEKLMEEQYVFPEEDLAEDIAKVSRRIENSLANL
                     EKTEVETVEFENRETADLIDSLYDILEREMEAQRYVKTNQSTIAEYIKHTTKNNRQLL
                     IELDHTSQSYTLNHNEIGRVRGFQTEVEEMERQNEQMIPQIRQHEIPYSEVRTFYKTV
                     FKVLEDIEKQQVEIDDSLHELRKGEKEAQEKIDTFEFKLRSLKRFVEKQRLPGLPNDY
                     LEFFFVATDRIEELSVVLNKIRVNMEEVNRLVALCEEDLELLDKKTHDLVDAAALTEQ
                     MLQYANRYRHTHEEVREAIDKSYYLFNKEYHYQEALDEIGTALERVEPGAFKRIEDFY
                     FNHPDLV"
     gene            9451..10593
                     /locus_tag="ACG842_001028"
     CDS             9451..10593
                     /locus_tag="ACG842_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HFQ0247589.1"
                     /translation="MIYFDNSATTPIFPQSLDAYVKTSQRIIGNPSSLHDLGNQANRL
                     LQQARKQIADLIHVEPEEIYFTSGGTEGDNWVLKGTMIEKREYGNHMIISGVEHPAVS
                     ETAEQLKELGIEVSIAPVDKRGFVRVDELKELIRKETVLVSVMAVNNEVGSIQPIQEI
                     SDLLDDFPKIHFHVDAVQAIGKVPFSQWLTERVDFATFSAHKFHGPRGTGFIYWKKGR
                     RLAPLLTGGGQERNQRSGTENVPGIVAMVKALRLSLEIKEQKPEQTQRLKVALINALK
                     TYEKVTIFSEGTDYAPHILCFALKGIRGEVLVHALEEKQIFVSTTSACSSRKHMASST
                     LHAMHVPNDLATSAVRISLDENNSLAEVEQFMIIFKQLYKKFSAIN"
     gene            10609..11820
                     /gene="thiI"
                     /locus_tag="ACG842_001029"
     CDS             10609..11820
                     /gene="thiI"
                     /locus_tag="ACG842_001029"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140741 - tRNA-uracil-4 sulfurtransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uracil 4-sulfurtransferase ThiI"
                     /protein_id="HFQ0247590.1"
                     /translation="MKYSEIMVRYGELSTKGKNRKSFIMQLAQNVRQALKDFPEIKIH
                     ADRDRMHLLLNGADGQLVIPKLAKIFGIQNFSPSIRVEKDVEVLKQAVQDIMKEIYTG
                     KETFKIIAKRSDHQFELTSNELNQTLGNAVFDIFPHIQVQMRQPDIPLRVEIRRDGAY
                     LSYETIKGAGGLPVGTSGKGMLMLSGGIDSPVAGYFAMKRGVEIEAVHFASPPYTSEQ
                     ALQKAKDLTAKLAPYVGTIHFIEVPFTEIQEEIKKSVPQGYWMTITRRMMLRLTDEIR
                     RIRHGLVILNGESLGQVASQTLQSMVAINEVTNTPIIRPVATMDKLEIIEVAEQIDTF
                     NLSIQPFEDCCTIFAPPQPKTRPQLEKVHQYEERLAIDEMIQRALAGLKMEEIQAESS
                     NEKTEEFSELL"
     gene            12393..13040
                     /locus_tag="ACG842_001030"
     CDS             12393..13040
                     /locus_tag="ACG842_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0051775 - response to redox state [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HFQ0247591.1"
                     /translation="MREKTDTLPKATAKRLPLYLRYLKMLDDSGISRIKSNEFSEITQ
                     IPSATIRRDFSQLGELGRSGYGYDVPFLIDVFNNILNTKEEKRIALVGYGNLGKALKH
                     NNFRRNENLNIVCVFDNDPALINRVIDGEMIYPIDRFAEIAKAKNVTVAISTVPSKYS
                     QSAIDEIVKGNVTAILNFAPDRVTVPAYVNVQYIDLTTELQTLIYFDENYSEVFS"
     misc_binding    13169..13385
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            13433..16078
                     /locus_tag="ACG842_001031"
     CDS             13433..16078
                     /locus_tag="ACG842_001031"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HFQ0247592.1"
                     /translation="MTEENNLSTKYNPQEVEAGRYQHWLEQDLFKPSGDKKAKPYSIV
                     IPPPNVTGKLHLGHAWDTTLQDMLIRQKRMQGYDTLWLPGMDHAGIATQAKVEAKLRE
                     QGISRYDLGREKFIDQVWEWKEEYASHIREQWAKMGLSLDYSRERFTLDDGLSEAVRK
                     VFVTLYEKGLIYRGEYIINWDPQARTALSDIEVIHKDIEGAFYHMSYELADGSGVVEI
                     ATTRPETMLGDTAIAVHPEDERYQALIGKKVILPLVNKQIPIIADEYVDMEFGTGVVK
                     ITPAHDPNDFEVGNRHDLPRVNVMDDDGTMNELAGKYAGMERFAARKAIVQDLKEIGR
                     LIKIDTMIHSVGHSERTGVVVEPRLSTQWFVKMAPLAKKAIDNQETDQAVEFFPPRFN
                     QTFLRWMENVHDWVISRQLWWGHQIPAWYHKETGEMYVGMEAPADSENWVQDPDVLDT
                     WFSSALWPFSTMGWPDTESEDYKRYFPTSTLVTGYDIIFFWVSRMIFQSLEFTGERPF
                     ENVLIHGLIRDEQGRKMSKSLGNGIDPMEVIEKYGADALRWFLSNGSAPGQDVRFSYE
                     KMDASWNFINKIWNASRFVIMNIEGMTVEEIDLSGEKSVADRWILTRLNETIERVTEL
                     FDRFEFGEAGRQLYNFIWDDFCDWYIEMSKEVLYGDDAVSKQTTRSILVHTLDQILRL
                     LHPIMPFVTEEIWEHIPHQGNSLVVAEYPVVRPEFDDETASKGMEVLKELIRAVRNIR
                     SEVNTPLSKPITLLIKTNDAKIDQFLVENTSYIERFCNPEELTISSEITAPDLAMSAV
                     LTGAEIFLPLAGLINIEEEIKRLEKELEKWTSEVKRVQGKLSNERFVSNAPEEVVEAE
                     RAKEKDYLEKQTAVKERIESLRTIQ"
     gene            16388..17701
                     /locus_tag="ACG842_001032"
     CDS             16388..17701
                     /locus_tag="ACG842_001032"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HFQ0247593.1"
                     /translation="MTIEEAIEWIHSRLPFGSRPGLDRVEALLEVVGHPERKVKTIHI
                     AGTNGKGSTVAYLRSLLEEAGLTVGTFTSPYIETFNERIAINGKSIPDNDLIQLVKKY
                     QPLIKQLDQFEAVAGITEFETLTAMAFDYFLMKKVDVAIIEVGLGGLLDSTNVAVPML
                     TGITTIGLDHTEILGDTIEEIAAQKAGIIKQGVPVVTGNIAPEAFTVIQQTAKAKNAP
                     LYVYGENYKINYLHPDEMWGEVFEFFNEIGKIPQLTTPLLGQHQAENAGVAIELYFVY
                     CQLEKLPFREKDVLKGLKKSRWPGRMERLSNEPLVVLDGAHNTHAMNRLVENLKKEFK
                     GYQINILFSALRTKNVREMLERLQQVPNVHIYLTTFEHPKAIELSEVSDLASERVSIV
                     SLWQFGLADILEKMTNEDVLVVTGSLYFISEVRHLLIQLGGDHEV"
     gene            17691..18344
                     /locus_tag="ACG842_001033"
     CDS             17691..18344
                     /locus_tag="ACG842_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFQ0247594.1"
                     /translation="MKYNAVIFDMDGLLFDTELVYYEATQVVADKMGFPYDKDLYLKF
                     LGVSDEEVWANYHMIFSEFGKTSVQRFIDDSYQETLERFSQGAVQMKPGVLELLSFLE
                     EHQIPKVVASSNQRAVIELLLKKNNLFERFETIVSAENVQRAKPDPEIFLLANQKLGT
                     AKKETLILEDSQNGILAASAAEIPVVMVPDLLPPSQELIKKTAGVVSSLHEIPALLK"
     gene            18399..19091
                     /gene="radC"
                     /locus_tag="ACG842_001034"
     CDS             18399..19091
                     /gene="radC"
                     /locus_tag="ACG842_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HFQ0247595.1"
                     /translation="MGKRLIKEVPTSSLPRERMEIYGAEALSDQELLAILLRTGQHPY
                     SVMSIAGNLLKTFGGLASLRQATLHELEEIRGIGRVKAIEIKALIELGRRIQTDHERA
                     APPVRTSYELAQQLILEMKDHKQEHLVCIYLDTKNQVILKKTVFIGSLNQSVAHPREI
                     FHYAVRYCAARIVLAHNHPSGNVIPSQQDMNFTKRIQKCGEMMGITVLDHLIIGRKRY
                     FSLREEGMMEEK"
     gene            19241..19441
                     /locus_tag="ACG842_001035"
     CDS             19241..19441
                     /locus_tag="ACG842_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFQ0247596.1"
                     /translation="MENGTVKWFNAEKGFGFISREDGSDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEESDRGPQAANVVKA"
     gene            complement(19695..21374)
                     /locus_tag="ACG842_001036"
     CDS             complement(19695..21374)
                     /locus_tag="ACG842_001036"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HFQ0247597.1"
                     /translation="MKLSIKNNETGVFAIGGLGEIGKNTYGVQFQDEIILIDAGIKFP
                     EDDLLGIDYVIPDYSYIIQNLHKVKALVITHGHEDHIGGIPYLLKQANIPIYAGPLAL
                     ALITNKLDEHGLLRDAELHEINEDTIIRFRKTSVSFFRTTHSIPDALGIVVKTPSGNI
                     VATGDFKFDFTPVGEPANLHKMARIGEEGVLCLLSDSTNAEIPTFTKSEKTIGHSILK
                     IFSKIEGRIIFASFASNIFRLQQAADAAVATGRKIAVFGRSMENAIVNGQRLGYIKVP
                     DGTFVDAHEINRLPANETMILCTGSQGEAMAALSRIANGTHRQIQIQPDDTVIFSSSP
                     IPGNTTSVNHLINLLSEAGAEVIHGKINNIHTSGHGGQEEQKLMLRLMKPKYFIPVHG
                     EFRMLKVHASLAQDVGVPAENCFIMENGDVLALTSDSARPAGHFVAGDVYIDGSGIGD
                     IGNVVLRDRHLLSEEGLVLAVATVDIKKKEILAGPDILSRGFVYMRESGEMINEAQRI
                     LFHALRECLNGPDCSEYKLKEAMTGALQPFLFERTERHPMILPMVMTPDEE"
     gene            complement(21376..21588)
                     /locus_tag="ACG842_001037"
     CDS             complement(21376..21588)
                     /locus_tag="ACG842_001037"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit epsilon"
                     /protein_id="HFQ0247598.1"
                     /translation="MIYKVYYQETKVRNPKREETKSLYIEAKSDVDARQRVEENTPYN
                     IEFVQLLEGSHLEYEKENADFSLVEF"
     gene            21981..23711
                     /gene="efrA"
                     /locus_tag="ACG842_001038"
     CDS             21981..23711
                     /gene="efrA"
                     /locus_tag="ACG842_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrA"
                     /protein_id="HFQ0247599.1"
                     /translation="MKLMWHYTMRYKKFLFFNFICVFGFILIELGLPTILARMIDVGI
                     RNDDFDYVKQQGIFMIVITVIGIILNILLGYFGSRITTNIVADIRDDLFEKIQSYSHQ
                     EYENLGVSSLITRTTNDAYQIMLFLQNILRIGFMAPMMFIVSLYMVMRTSVTLSLYVV
                     GALPLLLLAVVAIAKVSEPLSKKQQKNLDKINSILRENLSGLRVIRAFVNEKFEEKRF
                     AVVNENYASSSKSLFRLMAVAQPGFYFLFNIVMVLIIWSGAVQIDAGELRVGDLIAFI
                     EYIFHALFSFMLFASVFMMYPRAAVSAGRIQEALDAKPSIVEDPNGISDTKTKGYIEF
                     QNVTFAYPGHSQEPVIRNVSFTASPGETVAFIGSTGSGKSTLIQLIPRFYDVSEGKIL
                     IDGVDVRDYRLSKLRDKIGFIPQKALLFTGTIADNLRYGKEDATQEEMERAAEIAQAS
                     EFISRKPDGFNEHLSEGGSNFSGGQKQRLAIARAIIRRPEIYIFDDSFSALDYQTDAK
                     LRARLKKETTESTVLIVAQRVGTIMHADKIIVLNEGEVVGMGTHRELLEKCPIYYDIA
                     ASQLSKEELA"
     gene            23708..25468
                     /gene="efrB"
                     /locus_tag="ACG842_001039"
     CDS             23708..25468
                     /gene="efrB"
                     /locus_tag="ACG842_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_029485765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrB"
                     /protein_id="HFQ0247600.1"
                     /translation="MKKTFSSLKRLARYIRPYRETFLLVILFTVLTVAFNVALPYVTG
                     LPTTEISRNIANGESINFSYIFKCLIWITVVGIGYCISQLLSGVLMTNVVQSAMHDLR
                     RDIEEKINRLPVSYFDKNQQGNILSRVTNDVDAVSGALQQAFIGIVNAILGITLAAAM
                     MFYIQPMMALISMIMIPTSIWISKRVINASQKYFQSMQNSLGELNGYVQENMTGFSVL
                     KVYGREKETLEGFKKVNHSLKYFGFRAAFISGLMMPLVQLTAYATYIGMAVLGSFYVI
                     TGAIVVGQLQAFIQYIWQISQPMGNVTQLSSVLQSASAATKRVFEILDEPEEKENKVD
                     ASLPETILGDVTFDHVDFAYDPKKPLIQDLNFEVKAGQTVAVVGPTGAGKTTLINLLM
                     RFYDVDKGAIRIDGIDTKSMSRSDVRSLFGMVLQDAWLYQGTIADNIRFGKLDATDYE
                     VVDAAKTANVDHFIRTMPDGYDMAINSEGDNVSLGQKQLLTIARAVVSDPKILILDEA
                     TSSVDTRLEALIQKAMDRVMEGRTSFVIAHRLSTIRDADLILVMDQGQIIEKGTHESL
                     LAQGGFYEKLYNSQFAEEAE"
     gene            25563..25760
                     /locus_tag="ACG842_001040"
     CDS             25563..25760
                     /locus_tag="ACG842_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0247601.1"
                     /translation="MEQFLNEKITEAKREVDRLTHVCQTKPFDSADFFENEFQIERLA
                     AQIEVYELILKKVIEENIDVQ"
     gene            25913..26398
                     /locus_tag="ACG842_001041"
     CDS             25913..26398
                     /locus_tag="ACG842_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HFQ0247602.1"
                     /translation="MERFIASNATVIGDVTLSEDVTIWYQAVLRGDSNWIKIGQRTNI
                     QDGTIIHVDHDAPVDIAENVTVGHQCMLHGCTIEKGALIGMGTTILNHAVIGENSLIG
                     AGSLVTEGKVIPPNVLAFGRPAKVIRPLTKEEIQKNKENIQHYVEIGRMHAQGKFKEQ
                     R"
     gene            complement(26502..27095)
                     /gene="clpP"
                     /locus_tag="ACG842_001042"
     CDS             complement(26502..27095)
                     /gene="clpP"
                     /locus_tag="ACG842_001042"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HFQ0247603.1"
                     /translation="MNLIPTVIEQSSRGERAYDIYSRLLKDRIIMLSGQVTDDLANSI
                     IAQLLFLDAQDSEKDIYLYINSPGGSVTAGMAIYDTMNFVKADVQTIVMGMAASMGSF
                     LLTAGTKGKRFALPNAEIMIHQPLGGAQGQATEIEIAARHILQTRERLNKILAERTGQ
                     PLEVIEKDTDRDNYMTAEQAKAYGLIDEVMENSSSLN"
     gene            27275..27802
                     /locus_tag="ACG842_001043"
     CDS             27275..27802
                     /locus_tag="ACG842_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0247604.1"
                     /translation="MIVGITGLIGVLTGAVVVYIGMILQMRHLENKEQRRRELELKVK
                     EIETLNQLNKKINEILQKRTTLMEEYVSFDAFDDCYITIDDFAYLQSFSAQNSFYLPN
                     YILDELFKNISHRKVILSPDETMKIGGYAYKGGRVILENFTDSLTEMINEKKAQLKTM
                     TRQPIGFFSKDDVWS"
     gene            27893..28630
                     /locus_tag="ACG842_001044"
     CDS             27893..28630
                     /locus_tag="ACG842_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HFQ0247605.1"
                     /translation="MLQIYAHRGSSGTHPENTLPAFAEAVRVGADGIELDVHLSKDGY
                     LIVMHDEEVDRTTNGKGLIREKTLEELKKLNAGGKFSKEFPAAKVPELREVLGLLLQK
                     NYRGMLMIEIKTDQYEYPGIEEKLAETLKSRDWPFQHAYCSFHLDSLEKIALLEPEIQ
                     LDLLMSTSDKKIQLAAETPYIEGVHPKNTWLFEHPVQAADFPKAIRAWTINDEEEMAK
                     CQELKIDGIITDFPEKALRWKQSTKSR"
     gene            28634..29551
                     /gene="miaA"
                     /locus_tag="ACG842_001045"
     CDS             28634..29551
                     /gene="miaA"
                     /locus_tag="ACG842_001045"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HFQ0247606.1"
                     /translation="MEKVLVIVGPTAVGKTALSVELAQLFGGEIISGDSLQIYKNLDI
                     GTAKIKEEEMNNVPHHLIDVIEPTQRYSAVDFQHSGRQLITEIANRGHLPIIAGGTGL
                     YIQSLLYDYQLGATEEANPEIRKKYETLAETIGSQALWNQLKEKDPAAAEKIHWNNQR
                     KVIRALEVLETTGHSITAPKEEPKQLYDYLMIGLDTKRSILYERINQRVDLMLAEGLV
                     AEARKVYELGEVQASQGIGYKEFFPYFEGKETLEEATEQVKLHSRRYAKRQLTWFRNR
                     LDAQWFDLISEPNQKSQIEQTIKKWLEAE"
     gene            29553..30782
                     /gene="hflX"
                     /locus_tag="ACG842_001046"
     CDS             29553..30782
                     /gene="hflX"
                     /locus_tag="ACG842_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HFQ0247607.1"
                     /translation="MEEKVIIVGVETEANQRYFSESMEELVQLTNTASGEVVFTITQK
                     RPQVDRQTIIGKGKLQELVQQADAHEADLIIFNHELTPRQSQLITDAVGLPVIDRVQL
                     ILDIFAMRARSKEGKLQVELAQLEYLLPRLVGQGKTLSRLGGGIGTRGPGETKLETDR
                     RHIRNKIFAVKKELKEVEAHRERNRQKRKNSEIFQIGLIGYTNAGKSTILNLLTQADT
                     YSKDQLFATLDPLTKRWRFAEGFEITVTDTVGFIQDLPTQLIDAFHSTLEESQNMDLL
                     LHVVDASSPDRILQEQTVLKLMDELNMKEMPILTVYNKADQIDPAMFTPTLFPNVLIS
                     TQTKEGKMALIEAIKRQLMELMTPYTLSVPSNEGQKLSELRRQTMVLDEAYMEETNEY
                     EVKGFAKTNSKWLRKNQ"
BASE COUNT        10173 a         5267 c         6571 g         8787 t
ORIGIN      
        1 ccggagtaaa taaagcttat gcaaagaaaa agaaatccaa tcacttgggt ttctttttct
       61 tttattatta tgatgaatca agctttctgt ttttgtcacg tatgttgtaa tctttacgaa
      121 agagtagagt tggctactca taatcaacca tacttcccat catcgctaaa tacgaaataa
      181 atcagcattc ctacaccaat caaagcacaa gctgcaccaa gaaaagggat gacattccca
      241 aagttatctg tattcataag tatgccacca cccacaaaca aaaagccgcc agcaatcagg
      301 tttttcattt tattcatctc ctttcttacg ttatagtatt tccttctttc aatgagtatt
      361 ttgaaagcta tcttattgta tgatgttctt ctaggcctct tctttattat acaggccatt
      421 ttcgttatct catagaaata ttccttttta tctgaaaact attgtatacc gtatgacatt
      481 tttcttcatg aagttgtaag tatattctca ttcaaatgaa aatttcatca tagaagagat
      541 tgtatttcta aattcttaag agtaaaatac gaagtataac ttggatttgg aaggatgatt
      601 ggatgaaagc tattacgcct gatatgacaa caaaatttaa caaacagttt caagcaaata
      661 tcaaacagca ggcattgcag cgggcagttg tgaagaacgg aattactgct tctgctgaaa
      721 atgtttcagc acatgtggag aatacaccgg tattttctat agatttggct acggggaaag
      781 tggctaatca aatgcaatcg gggcgttgct ggatgtttgc tgcactgaac acattccgtc
      841 ataagatgct gaatcaattc catttaaaag agtttgaatt atcacaaaat tacacttttt
      901 tctgggataa atatgaaaag gcaaattatt tctatgaaaa catcattgca acaggaaaag
      961 aggcaatcga tagccggaaa gtagctttct tactcgctac tcctcaacaa gatggcggcc
     1021 aatgggatat gatcgtttca ttattccaaa aatatggcgt tgtgcctaaa tcggtgatgc
     1081 cggaaagtgc gaatagttca aattctcgag atttgaataa ctacttgaac aaattactgc
     1141 gtaaagatgc agtagtgtta cggaaaatgg ttgctcaagg agaaacatta gacgctattg
     1201 aagaaaagaa agaagaaatg ctagaaagca tttataactt cttagctatc tcattaggaa
     1261 cacctccaaa agaattcgat tttgagtacc gcgatgaaga aaagaactat catcttgacc
     1321 gaggactgac accacaaatt ttttatgata aatacatcgg tgtaaaatta gatgattatg
     1381 ttagcgtgat caatgcacca acaaaagaca aaccattcaa tcgttcttat acagtagaga
     1441 tgctaggaaa tgtcgttggc gggaaagaag tcaaatatct gaatgttgat atggaaactt
     1501 tcaaaaaatt agcgattgct cagcttgaag aaggagaatc tgtttggttt ggttgtgatg
     1561 taggtcaatc ttctactaga actagtggca tcatggcctt agatgcttat tcaatggatg
     1621 atctatttga tacggatttt acaatgacta aagctgaacg tttagatttt ggagaaagtc
     1681 tgatgaccca tgcgatggta ttgaccggtg ttgacttagt agacgggcaa tcaacaaaat
     1741 ggaaagtcga aaatagctgg ggcgaaaaag ttggaaagaa tggcttcttt gttatgagtg
     1801 atgcctggat ggacgaatat acgtaccaaa tcgttgtaag aaaagattta ttgacgaaag
     1861 agcaattaaa tgcgttcaat gaagaaccaa ttgttctatc tccgtgggat ccaatgggtg
     1921 ctttagcata gacgagtatt tcaatgaaga gtatgtagta aagataaaaa gagctggctt
     1981 aagccagctc tttttatctt taaagtccat tccctgtttt ttatggtccc tgtgctataa
     2041 ttctaaataa aggataagga ttggaaataa tccttttatc attaaaaata tgatgacgaa
     2101 gtagaataaa attgggagta ggggggaaag tcatggatat ccaaactgtt ttatctgatc
     2161 tgaaattaaa tggtaaagtg attccagttg ttggtggcga tgttaatcag acctatcgta
     2221 taaaaaccga acatcgtgct tattttttaa aaatccatcc gaatgtaaaa aaaggatttt
     2281 ttgaagcaga agtagacgga ttaaaagaac tgtcagcatt cgtacgtgta ccggatacgt
     2341 atatgctagg tgaaacaagt gaaggcgctt atttgttaat ggaatggatc gagccgggaa
     2401 aaggggatca acgagatctt gctgcagctt tagctaatct ccatcagcaa acagcgccac
     2461 aatttggttt tcgtaaagat aactatttgg gtacacttgt tcagaaaaat tcttttgaag
     2521 aagattggtg gacttttttc tttaaagatc ggcttgaatc ccagatttct ttggcagaag
     2581 aaacgaatcg atggaatgta cagcgtcaag aaaagtatct gcgatttaaa gaacgtgtat
     2641 tgaaatcggt tgaaccaaaa aagatcactc cacgcctctt gcacggagac ctgtggagtg
     2701 gtaatgtgtt ctttgaccag caagggcatc cagtttttgt cgacccagct gtttcttatg
     2761 ggaatcggga acaagatatt gcaatgagcc agttattcgg tggtttccga ccggaatttt
     2821 tagatgctta tcaaactata tttccattag aaaagggatg gaaagaccga ctgccgattt
     2881 accagttata ttatcttttg gcacatttga atatgtttgg agaaagttac ggttcccaag
     2941 tagatcaatt actagaaaat ttttaagagg agggcgtgtt tttggcaaaa aaatatacca
     3001 aaaagcatga agtttcatat tatgagtgtg acatcaacca aacgatgaca tttccttcga
     3061 tgttaggagt agttatcaaa acgtctgaag atcaaagcga ccagttggga agaggcagtg
     3121 aattcgtcaa tagctttgga cttacttggg tcatcacgaa ttattccatg gagattaatc
     3181 gtctccctag agtcggtgaa gtaatctcag taacgactca agccatggag tacaataagt
     3241 ttttctgtta tcggaacttc tggatccatg atgaagatgg gaaagaatta gtcaaaatag
     3301 aatctgtttt cgtattgatg gattttgtga atcgaaaaat gagcagtgtt aatgaagaga
     3361 tcattgctcc ttttgagagt gagaagatca agaaaatcaa gcgtcaggaa aaaattgaaa
     3421 agattgattc gggcttcatg ttgccatatc gtgttcgttt ttatgatatc gatagtaatc
     3481 agcatgttaa taatgcgatg tattttaatt ggatcattga cgtattagga tatgactttt
     3541 tgactacgca tgttccagaa aaagtaatta ttcgatttga taaagaagta gagtacggga
     3601 atgagatcga aagccatttt gaacaaatcc ctatcgaaaa tggtgtcaaa acaaaacatg
     3661 aaatccgaat gcaagaacag ctgtattgtg aagcaaatat tatatggaaa aataaataaa
     3721 aaagaggttg tgacagaagg gggcaacttc aagaaataag aagaaattta cgaaaattgt
     3781 ttctcaaatt tttgtaaatt ttcgacttag ttttcgaaga agctattttt gtcacagcct
     3841 ctctttttaa gtttagctat tagataatga tgtttagttt tggataaacg aaatcaattc
     3901 ttcacccgac aaaccatagt ttacagcaat caatagtttt aaacgtgctt tttgactatt
     3961 aatactgtta cagaaaataa tccccatttt ttctagttct tttcccccgc cttcataatc
     4021 ataaacaggc tctgcgattc cattgaaaca acgagaaaca agtactacag ggattttttg
     4081 gacaagaagc ttttccaaag cagctaaggc ttgtggaggg agattccccg cacctaaagc
     4141 ttcgattacc aaaccgtcta ctttcgtatg ggcgatggct tccaataagt ccccttgcat
     4201 cccagcatat gctttaacga tcgggatagt cccatctaca gcggaaatgt ccagtcgctt
     4261 tgtttctaaa agttcctgca agaataaaat tcgattctta gttactaagc cgatgggacc
     4321 caatgtaggt gttcggaaag tagcaacatt tgtggtatgg gtctttgtca cgtagcgtgc
     4381 agaatggatc tcatcgttca tgaccaccaa aaccccctta tctagcgctt cttcacaact
     4441 tgcgacacga atcgcacttt caaaattata aaggccgtca cttccaagtt cattacttga
     4501 acgcattgcc ccagtcaaaa caattggtaa tttgttgcca atcgttgtat caagaaaaaa
     4561 agcagtttct tctaatgtat ctgtaccgtg tgtgatcacc actccggata tacctgatgc
     4621 ataagcggac aagatacgtt cttttaatac caacatgtga gaaggcatga tgtgtggtga
     4681 gggaagattg aagatatctt caactaataa ttctacatgc ttggggattg tgatttctgc
     4741 atctaataat gggtttgcca catcaggact aacaccgccc gtgagcatgt tttctttcat
     4801 ggcgatcgtt ccgccggtgt gtagtacaag tatttttttc attttacaga cttccttacg
     4861 aataattttg catagccgtt aaatcttatt gtatactata taaaagagtt ttgctatcag
     4921 tgaagaataa cgaatagcct aacttatcat aaaaaagctg accttattaa aatggaaaag
     4981 actcaacctt gacctcctaa tacaatggag tatctggttg agtttctttt acaagaaagc
     5041 agccaaggaa atcaatcacg aaggtggccg gaaatgatca gaatcggatt aacatcgttt
     5101 aaagaacatg acaaattaac tggtaaaaag aataacactc tttatgaata tgcagggttt
     5161 ctacccttag tggaattaga tactgcctat tatggaattc cccgaaagtc ctcagttgag
     5221 aattggataa gtgaagtacc tgaatcattt cgctttgtag tcaaagtcta tcaaggaatc
     5281 agcggtcaag gagactggaa gccttattat cagaccgaag aagagatgat tgcgcatttt
     5341 ttagatgtta tgtcgccaat ggtagatgct ggaaagatgt tttgcttttt agtccaattc
     5401 ccctcatcat tcaaatgtac gaaagaaaat gtccagtact tgcgcaaact tagaaagtgg
     5461 tttgagggtt atcctgtttc tatagaattg cgggattatt cttggtacag cgatgaattt
     5521 cttgaacgta caagaaactt tatgtctaga gccgcttttt ctttagccat catcgatgag
     5581 ccacagttgc tgaatacaac gattcctttt gatccatttg tcaccaatcc gaactttgct
     5641 ctttttcgat tccatggtag gaatgcgcaa ggatggcaag caaatgataa agagtggcgt
     5701 aaaaaaagaa cgttatatcg ttattcagaa aaagaactgc aagaactagc acatgaagtc
     5761 aaagtagttg attctaagac aaaagaggta ggtgtgattt ttaataataa ttctggtggt
     5821 gatgcggctg aaaatgcatt agcactgcaa aaaatattaa atatcaagcc agatggactc
     5881 aatccaaccc aaatggatct gttttaaggg cttattttat tcaagaaagg ggcgaagtct
     5941 atgatcaaag ctattttttt tgatattgac ggaacattgg tcaccaatcg ttcaaaagcg
     6001 ttggaatcaa caaaacaagc aattgaatat gctcggaaaa acggcatttt atgcggtgta
     6061 gcaacggggc gcagcccagt aaagatcaaa gaaatcattg atgaattgga attagatatg
     6121 tatgtcgtgt ataatggtca actggttttt actgcagatc gcacgattat cgaccaccct
     6181 tttgaacaaa aagtattgga acatattgtt gaatttgcag atgagaacca tcgacaaatc
     6241 gtttttggtg cgagaaatcg gttagatgga tcaacgacga tgctattagg acaatcgatt
     6301 tttatcaagc gactagtcag ctttttaccg cgtaaattcc cggtacgtct gatgaaaaaa
     6361 atcctccagg tttttagccc tcaccgacaa aaggatcgtt atgaaacgtt agagatatta
     6421 aaagaaccaa tttatcaatg tatattgctc agtccggagt cagaacagaa aaagttagaa
     6481 catcgctttc ccgagtgtac gtttcagcgt tcaaatacgt atactgtaga tatcatccca
     6541 aaaggtggtt caaaattact aggcatccaa gctttcgcaa atgctgtcga aattgaaatg
     6601 gaagaaatta tggcattcgg cgatcattac aatgatatcg agatgctgaa aggtgtagga
     6661 attggagtgg ccatggggaa tgctcagatc gaagtaaaac atgctgcaga ctttgtgact
     6721 caatcaaatg aagaagatgg gatatacgaa ggactgaaac acttcggtct gatttattag
     6781 tcaatgaaag aaggaaaaaa tcagtatgga agcagagaca ccttttcaaa aagtagaaac
     6841 attccacaaa acttttgatc caagaagacc agagataccc acggctttta cagaaaatga
     6901 agctcataca cgtgctgcgt tcaaaataga agaacttgtt gaatttgttg cagctacttc
     6961 acctaataaa gaacaactgc atgaagctgt tatgctgctg cacgaagcat tagataaagc
     7021 agaagaaaaa atacttggta aaacacagtg gggaagtgat ccgctggttg aacaagtaga
     7081 tgcactgaca gatatccttt actttactta tggttctttt tcattactag gaatagtgcc
     7141 tgatcgaata ttcgaaatcg tccatcaggc aaatatgggg aaattatttc cagatggaaa
     7201 acctcattat gaccctgtaa caaataaagt gttgaaacct gaaaactggc agatggaata
     7261 tgctccagaa cctaaaatcg cggcagaaat ccaacgtcag attcaagaag cgattgctaa
     7321 gaagaaatcc gctgatatag gcatgtgcac aaatgaaaaa gagtgatcag agtttcacta
     7381 tttttgttat ctttatacga tggaaaaaca aaagattttc gtttaagtta gtgaatgcat
     7441 gaaaatagta tgctataatc tagaatagat gtcttttgca cgagagacgt attatttgga
     7501 tttatgccaa gcagaacgcc atctgttgca taaaagatgc atactgaaaa cggaggaacc
     7561 ataaatggga aataatgtta tcatcggaat cgtcattgca atcattatag tcgccattgt
     7621 cgtctttttg gttagttact tgatgaagaa aaaaaaccaa gatcgattgg atgccttaga
     7681 aaaacgcaag gaagaattat ttgacttgcc agtgattgaa gaagtcgatg atgttaaaaa
     7741 aatgcatctg gttgggcaaa gccaaaatac ctttcgtgaa tggagtcaaa aatggaatga
     7801 catatctact tcctcgttcg ctgaactaga aagtcaaatt ttcgaagtgg aaaacctcaa
     7861 tgaaagtttt cggttcttaa aaggaaaaaa agcagttgtt gaagcagaag ctacaatgga
     7921 ccaaatggag aaacaagtga cggaaatccg tgaaggtcta aaggaattgc gggaaacgga
     7981 agaacgcaac tcactagctg ttcagcaggc acttgacgtc tatgaagaac tgaaagaaaa
     8041 agtcagagaa gacgagcatg aatacggacc agctcttttt gaattgcaaa aacagatcaa
     8101 agtgatcgaa aaagaattta cgcagtttgt gactttaaac acttctggag accctgttga
     8161 agcccgcgag gttttggata aagcagaaaa acatacgtat gaagtggaag ctctgatgaa
     8221 aaaaattcca ccgctgtatg aggagctcca taccactttc cctgaacaac ttgaagaaat
     8281 cagtgatact tacgagaaat tgatggaaga acaatatgta ttcccagaag aagatcttgc
     8341 tgaagatatc gcaaaagtaa gtcgaagaat tgaaaattca ttagccaatc ttgaaaaaac
     8401 agaagttgaa acggtcgaat ttgaaaaccg tgaaacagct gatttgattg attcgttata
     8461 tgatatctta gaacgggaaa tggaagctca gagatatgta aaaactaacc aatcaacgat
     8521 tgcagagtat atcaaacata caacaaagaa taaccgtcaa ttattgattg agctggatca
     8581 cacatctcaa agctatacat tgaaccacaa tgaaattggc cgtgtacgag gtttccaaac
     8641 cgaagtggaa gaaatggaac gtcaaaatga acagatgatt ccacagatcc gtcaacacga
     8701 aattccttat tcagaagtgc gcacgttcta taaaacagtc ttcaaagtct tagaagatat
     8761 cgaaaagcaa caagtagaga tcgacgactc attacatgaa ttgagaaaag gcgaaaaaga
     8821 agcgcaagaa aagatcgata cattcgaatt caaattgcgc agtttgaaac gttttgtaga
     8881 aaaacaacgt ttgcctggct tgccgaatga ttacctagaa ttcttttttg ttgctactga
     8941 ccgtattgaa gaactaagtg ttgtcttgaa taaaattcga gtaaacatgg aagaggtcaa
     9001 tcgtttagtt gctctatgtg aagaagatct agaattatta gataagaaga cacatgattt
     9061 agtagatgca gcagcattaa cggagcaaat gctccaatat gcaaatcgtt atcgtcatac
     9121 acacgaagaa gtgagagaag caattgataa aagctactat ttattcaata aagaatatca
     9181 ctatcaagaa gcattagacg aaatcggtac agcattagag cgagtagaac ctggtgcttt
     9241 caaacgcatc gaagattttt attttaatca tccagactta gtataaagca aaaaagttag
     9301 taaaccagca agagcaagac ggattattgt cttgttcttg tctttttttg tatatttgaa
     9361 aattgaaaca agtgaaaatt tcacctgctc ttgaaagcac cttggacaac cgttataata
     9421 gaaaagaatt tcaaaaaaga ggggtttctt atgatatatt ttgataacag tgcaacaacc
     9481 ccaatctttc cgcaaagctt agatgcatat gtaaaaacca gtcagcggat tattgggaat
     9541 ccatcaagct tgcatgattt aggcaatcaa gctaaccggt tgttgcagca agcaagaaag
     9601 cagatcgctg atttgatcca tgtagaacca gaagaaatct attttacttc aggcggaaca
     9661 gaaggagata actgggtatt aaaaggaacg atgatcgaaa aaagagaata tgggaatcat
     9721 atgatcattt caggagtcga acatccggcc gtcagcgaga cagcagaaca attaaaagaa
     9781 ttaggaatcg aagtaagtat cgccccagtg gataaacgcg gatttgtacg tgtcgacgaa
     9841 ttgaaagaat taataagaaa agagacagta ttagtctctg ttatggctgt gaataatgaa
     9901 gtaggatcga ttcagcccat ccaggaaatc agtgatcttt tggatgattt tcctaaaatt
     9961 catttccatg tagatgctgt tcaggcgatt ggaaaagtac cattctcgca atggttgaca
    10021 gaaagagttg attttgcaac attttcagct cataaattcc atggcccacg gggaactggt
    10081 ttcatttatt ggaaaaaggg tcgtcgttta gcaccattac tgacaggtgg agggcaagaa
    10141 agaaatcagc gaagtgggac agaaaatgtt ccaggtattg tggctatggt taaagcttta
    10201 cgtttatcat tagagataaa ggaacaaaaa cctgagcaga cacaacgctt aaaagtagct
    10261 ctaatcaatg cgttgaagac gtatgagaaa gtgacgattt tttcagaagg aactgactat
    10321 gcaccgcata ttttatgttt tgctctaaaa ggaattcgcg gcgaagttct cgtccatgcc
    10381 ttagaagaaa aacaaatatt tgtttctact actagtgcct gctcctcacg taaacatatg
    10441 gcaagcagta cattgcacgc aatgcatgta ccaaatgatc tggctacatc tgctgttcga
    10501 atcagcttag atgaaaacaa ttcgcttgca gaagtcgagc aatttatgat tattttcaag
    10561 cagctttata agaaattttc tgcaatcaat taaaagagga gtgttcttgt gaaatacagt
    10621 gaaattatgg tccgttatgg ggaactttcg acaaaaggaa agaatcgaaa aagctttatc
    10681 atgcaactag cacaaaatgt cagacaagct ttgaaagact ttccagaaat caaaatccat
    10741 gctgatcggg accggatgca tcttttattg aatggcgcag acgggcagct agttattcca
    10801 aaactagcaa aaatatttgg gatccaaaat ttctctccaa gtattcgtgt agaaaaagat
    10861 gtcgaagttt tgaaacaagc tgttcaagac atcatgaaag aaatttatac tgggaaagaa
    10921 acatttaaaa ttatcgctaa aagaagcgat caccaatttg aattgacaag taatgaattg
    10981 aatcaaacat taggtaatgc tgtttttgat attttcccgc atatccaagt tcaaatgcgt
    11041 caaccagata ttccattacg tgtagagatt cggcgtgatg gcgcttatct atcgtatgaa
    11101 acgatcaagg gagctggagg attgcccgta ggaacaagcg gaaaaggaat gcttatgctt
    11161 tctgggggga ttgattctcc tgtagcaggg tactttgcca tgaagcgggg agtggaaatt
    11221 gaagctgttc attttgctag tccgccgtat acaagtgaac aagcactgca aaaagcaaaa
    11281 gatctaacag ccaaactagc accttatgtg ggaacgatcc attttatcga agtgccattt
    11341 accgagattc aagaagaaat caaaaagtca gtgccacaag gatattggat gacaattact
    11401 cgtcgaatga tgttgcgttt gaccgatgaa attcgccgta ttcgtcatgg tttagtcatt
    11461 ttgaatggtg aatcattagg tcaagtggct tcacaaactt tgcaaagtat ggtggctatc
    11521 aatgaagtaa cgaatacgcc aatcattcgg cctgtagcga caatggataa acttgaaatc
    11581 attgaagtag cagaacagat cgatacattt aatctttcta tccagccttt tgaagattgc
    11641 tgtacgattt ttgctcctcc acagccaaaa acacgccctc aactggaaaa ggtccatcaa
    11701 tatgaagagc gtttagcaat agatgaaatg atccaacgag cactagctgg cttgaagatg
    11761 gaagagatac aagcagaatc gtctaatgaa aaaactgaag aattttcaga attgctataa
    11821 gaggttatga aaatagtgtt ttgaccagag tattagagaa acaaaacgaa aaaatagctc
    11881 catgtatttt accgtttttg aactaatgtc gaagattagc tgattgaata ccatttatca
    11941 agaagttatg gaaaagccgg ttctttttta ctaactgaaa aataaaattt tatttagaga
    12001 tcaagctaca gttgataaat gatataaaaa agagaatttc ccatgaaata gcgaggttgt
    12061 gacagaagtg ggcagcttca agaaataaga aggaatttac gaaaattgct tttcaaattt
    12121 ttgtgaaatt tcggcttatt tttcgaagaa gttacttctg gaacaccgtg tatcagaggt
    12181 tgcgaaaaag ctgccctgca tcaagtaata agaacagcca aacaaaaata gttcctcata
    12241 ttttacgttt ggttgagctt attaccgcag atgcaagctt ttgaacaccg ttttatttgt
    12301 ctcacaccct tttccttcct acttgtaacc ttgctcccgt gttagaaaca tgttacacta
    12361 aatatgtgaa attattaaca aggagcgtcg ttatgcgaga aaaaacagat acactgccaa
    12421 aagcaactgc gaaaagatta cctttatatt tacgctactt gaagatgctg gatgactcag
    12481 gtataagtag aatcaaatca aatgagttca gcgaaatcac ccaaattcct tctgcaacga
    12541 tccgcagaga cttctcgcaa ttaggcgagc taggcagaag cggatatggc tatgatgtcc
    12601 cttttcttat cgatgtattc aataatatcc ttaatactaa agaagaaaaa cgaatcgcat
    12661 tagtaggata tggaaatctt ggcaaagcat tgaaacacaa caattttaga agaaatgaaa
    12721 atctaaatat cgtgtgcgtc tttgataatg atccagcact gatcaatcga gtaattgatg
    12781 gagaaatgat ctatccgatt gatcgtttcg cggagatagc caaagcaaaa aatgtgacag
    12841 tagcaatctc tacggtacca agtaaatatt cacaatctgc tattgatgaa attgtcaaag
    12901 gaaatgtaac cgctatactt aattttgcgc ctgacagagt aactgtccca gcttatgtca
    12961 atgttcaata cattgatttg acaaccgagt tgcagacgct tatttacttt gatgagaatt
    13021 actcagaagt tttttcttag gaaaatttga gtaggcttat tgatcttgcg agaactcgtc
    13081 gtaattcttt caaaaatttt ttagtactaa atgaagtggt tttttcacga ccacttgttg
    13141 actaaaaaaa aaaaccattg tagaatgaag cataacgctg aacaagagga gtaactggca
    13201 cattcattct tagagagaga actatctgct gaaaggttct caatgacaac cagtgaaggt
    13261 agcttgggag tttctgtctt gaatggagta gagatagacg agtcgtgatc gttaactcac
    13321 ttgagaggca ataatgattg attattgcaa tttaggtggt accgcgtaaa tcatacgtcc
    13381 tagaatacga aagtatttta ggacgttttt tagtttaaag gaggaaaaca gcatgacgga
    13441 agaaaataac ctatcaacaa aatataatcc gcaagaagtt gaagctggac gttatcagca
    13501 ttggttagaa caagatttgt ttaaaccgag cggagataaa aaagcaaaac cttattcaat
    13561 cgtaatcccg cccccaaatg ttactggaaa acttcattta gggcatgctt gggatacgac
    13621 cttgcaagat atgctgattc gtcaaaagag aatgcaagga tatgatactt tatggctgcc
    13681 tggtatggac catgcaggaa tcgcgacaca agcaaaagta gaagcgaagc ttagagagca
    13741 agggatttct cgttatgatc taggtcgtga aaagttcatc gatcaagtgt gggaatggaa
    13801 agaagagtat gcgagccaca tccgcgaaca gtgggctaaa atgggcttat ctttagatta
    13861 cagtagggaa cgttttacat tagatgatgg attatctgaa gcagtacgta aagtttttgt
    13921 taccttatac gaaaaaggtt tgatttatcg tggtgaatat atcatcaact gggatcctca
    13981 agcacgaaca gctctatctg atatcgaagt tattcataaa gatatcgaag gcgcctttta
    14041 tcatatgagc tatgaactag cagatggatc aggcgtagta gaaattgcga caacccgtcc
    14101 agaaacgatg ttgggagata cggcgattgc tgttcatcca gaagacgagc gttatcaagc
    14161 gctcatcggc aaaaaagtga ttctaccttt agtcaataaa caaatcccaa tcattgcaga
    14221 tgaatatgta gatatggaat tcgggacagg tgtagtaaaa atcactccag cacatgatcc
    14281 aaacgacttt gaggtaggta atcgtcatga tcttccacgc gtaaatgtta tggatgatga
    14341 tggtacgatg aatgaactag caggaaaata tgctggaatg gaacgttttg ctgctcgtaa
    14401 agcaatcgtg caagatctga aagaaatcgg tcgattgatt aaaatcgata cgatgatcca
    14461 cagtgtgggt cattccgagc ggacaggtgt cgttgttgaa cctcggctgt ctactcaatg
    14521 gtttgtaaaa atggcgccgt tagcgaaaaa agccattgat aaccaagaaa cagatcaagc
    14581 agtggaattc ttccctccaa gattcaatca aacgttttta cgttggatgg aaaatgtcca
    14641 tgattgggta atctcacgac aattatggtg gggacatcaa attcctgcat ggtatcacaa
    14701 agaaacaggt gaaatgtatg tagggatgga agcaccagcg gatagtgaaa actgggtaca
    14761 agatcctgat gttttagaca cttggtttag ttcagctttg tggcctttct caacaatggg
    14821 ctggccagat acggaaagcg aagattataa gcgatacttc cctactagca ctttagtaac
    14881 cggttatgac atcattttct tctgggtaag cagaatgatt ttccaaagct tggaatttac
    14941 tggcgaacgt ccatttgaga atgtcttgat tcatggattg attcgtgacg aacaagggcg
    15001 gaagatgagt aaatcattag gtaatggaat cgatccgatg gaagtaatcg aaaagtatgg
    15061 agcagatgct ctccgctggt tcttatcgaa tggttctgca ccaggacaag atgtccgctt
    15121 cagctatgaa aaaatggatg cttcttggaa ctttatcaac aaaatctgga atgcttcacg
    15181 tttcgttatc atgaatatcg aaggaatgac cgtagaagag atcgatttat ctggagaaaa
    15241 atcagtggct gaccggtgga tccttactcg tttgaatgaa acgatcgaac gagtaactga
    15301 gctatttgat cgttttgaat ttggtgaagc aggtcgacaa ctgtataact ttatctggga
    15361 tgatttttgt gactggtaca tcgaaatgag taaagaagta ctttatggag atgatgctgt
    15421 ttcaaaacaa accacccgta gcatcttagt ccatacactt gatcagatcc tacgtttatt
    15481 gcatccaatc atgccattcg ttacagaaga aatatgggaa catattcctc atcagggaaa
    15541 ttctctagta gtggcagaat atcctgttgt acgtccagag tttgatgatg aaacagcttc
    15601 taaagggatg gaagtattga aagaattgat tcgagccgtt cgaaatattc gttcagaagt
    15661 gaatacacca ttgtctaaac caattacgtt gctaattaaa acaaacgatg ctaaaatcga
    15721 tcaattttta gttgaaaata caagctatat cgaacgcttc tgtaatcccg aagagttgac
    15781 gatatcaagt gagatcactg cgccagatct agcgatgtca gctgttctga caggtgcaga
    15841 aatcttcttg ccgttagcag gattgatcaa tattgaagaa gaaatcaaac gattagaaaa
    15901 agaactggaa aagtggacaa gcgaagtaaa acgtgtccaa ggaaaacttt ctaatgaacg
    15961 ttttgtttct aacgcgccgg aagaagtagt agaggcagag cgtgcaaaag aaaaagatta
    16021 tttagagaag caaacggctg tcaaagaacg aatcgaaagt ttgcgtacta ttcaataaga
    16081 atagaaaaag agagtgtgaa atcagctagc tattactaaa agctagctga ttgaacatcg
    16141 ttacaggaaa gagtttgagc ctcactctta gcttcgattc ataaggagat gtgacataaa
    16201 ttttgtcgca tctctttttt agtctactct tattatgtag gagaaggagg gaggtggatc
    16261 atgtatcaag agtcaataaa gtagttgata aaatcgttcc tctctctgtt tcttttctct
    16321 ttgtcatact tttatttttc ttatgtgttc tatttgttat aataaaagag aaatggggga
    16381 attgttgatg acgattgaag aagcaataga atggatccat agtcgattac catttggttc
    16441 acgtcctggc cttgaccgag tcgaagcttt attagaagtg gtagggcatc cagagagaaa
    16501 agtaaaaacg atccatatcg ctggaacaaa cggaaaaggt tctactgtag cttatttgag
    16561 aagcctatta gaagaggctg ggctgacagt aggtacattt acttcacctt atatcgaaac
    16621 gttcaatgaa cggatcgcaa tcaatggcaa aagcattcca gacaatgatt tgatccagct
    16681 tgtaaaaaaa tatcagccac tgatcaaaca acttgatcaa tttgaagcag tagctggtat
    16741 tactgaattc gagacattaa cagcaatggc atttgattat tttttgatga agaaagtgga
    16801 cgtagcgatt atcgaagttg gcttaggcgg cttattggat tctaccaatg tagctgtacc
    16861 aatgctgacg ggaataacaa cgattggact tgatcatacc gaaatacttg gagatacaat
    16921 tgaagaaatc gctgcacaaa aagctggaat catcaaacaa ggcgttccag tagtaacagg
    16981 aaatattgcc ccagaagctt ttactgtcat ccaacaaaca gctaaagcaa aaaatgcacc
    17041 gctttatgta tacggggaaa attataaaat aaactatctg catcctgatg agatgtgggg
    17101 agaagtattt gaatttttta atgaaatagg aaagatccct caattaacca ctccgctttt
    17161 agggcaacat caagctgaaa atgcaggagt agccatcgaa ttgtattttg tttactgtca
    17221 attggaaaag ctgcctttcc gagaaaaaga tgttttgaag ggattgaaga aatctcgatg
    17281 gccaggaaga atggagcgtt tgagtaacga gccattagtt gttttagatg gtgcccataa
    17341 tacgcatgca atgaaccggc ttgttgagaa tttgaaaaaa gaattcaaag gatatcaaat
    17401 caacattttg ttttccgcct tacgaactaa aaatgttaga gaaatgctag aacgattgca
    17461 gcaagtacca aatgtccata tttatttgac gacctttgaa catccaaaag ccatcgaatt
    17521 aagtgaagta tcagatttag caagtgagag agtatcaatc gtttcattgt ggcaattcgg
    17581 attagcagat atattggaaa agatgacaaa tgaagacgta ttagttgtga ccggttcact
    17641 ttatttcatt tcagaagttc gccatttatt aatacaatta ggaggagatc atgaagtata
    17701 atgcagtgat ttttgacatg gatggtttgc tgttcgacac agagttagtg tattacgaag
    17761 ccacacaagt agtagcagac aagatgggtt ttccttatga taaagacctg tatttaaaat
    17821 tcttaggtgt ttcagatgaa gaagtatggg cgaattatca tatgattttc tcggaatttg
    17881 gcaaaacaag tgtacaaaga ttcatagatg attcatacca ggaaacgctt gaacgttttt
    17941 cgcaaggagc ggtacaaatg aagcccggtg tgttggaact cctctctttt ttagaagaac
    18001 atcagattcc caaagttgtc gcttccagta accaacgagc agtcattgaa cttttattga
    18061 agaagaacaa tctgtttgaa cgatttgaga cgattgtttc tgcagaaaat gttcaacgag
    18121 caaaaccaga cccagaaatt ttcttgttag ctaatcagaa gctaggtaca gcgaagaaag
    18181 aaacattgat tttagaagat tcacaaaatg gtattttagc agcttctgcg gctgagatcc
    18241 cggtagtcat ggtaccagat ctgcttccac catcccaaga attgatcaaa aaaacagctg
    18301 gggttgtttc ttctttacat gaaatccctg cattgttaaa ataaaaatcc atctcttttc
    18361 gtatcttatt ttagataaga tagaaaggag atgttttttt gggaaaacga ttgatcaaag
    18421 aagtacctac tagctcttta ccaagggaac gtatggaaat ttatggcgcg gaagctttat
    18481 cggatcagga gcttttggca atcttactgc gtaccggtca acacccttat agtgtgatgt
    18541 cgattgctgg aaatctgtta aaaacgtttg gaggactggc ctctttacga caggctactt
    18601 tgcatgagtt agaagagatt cgagggatag gacgcgtgaa agcaatcgaa atcaaagcct
    18661 tgatcgaatt aggtcgacga attcagacgg atcatgaaag agcggctcca ccggttcgta
    18721 ccagctacga attggcccag caattgattc tggaaatgaa agatcacaaa caagaacatt
    18781 tggtttgtat ttatttggac acgaagaatc aagtgatctt aaaaaaaaca gtgttcatcg
    18841 gttcgctgaa tcagtcagtc gctcatccta gagaaatttt ccattatgct gtacgttatt
    18901 gtgcagcaag gatcgtttta gcgcataatc atccttctgg taatgttatt ccatcgcagc
    18961 aggatatgaa ttttaccaaa aggatacaaa agtgtggaga aatgatgggg atcactgtac
    19021 tggaccactt gatcatcgga cgcaaacgat attttagttt gagagaagaa ggaatgatgg
    19081 aagagaagta agttgctttc ttgcaaaaaa aatgaagcaa gtgtacaatt atattgtaat
    19141 tttgtttggg aatagaagtt gaaacatctg ttgcacctct atcgtacacg agcaatgtta
    19201 caatttaaat tagtgctttt ggcacgagga ggatattatt atggaaaacg gaacagtaaa
    19261 atggttcaac gctgaaaaag gatttggatt tatctcacgt gaagatggaa gcgacgtatt
    19321 cgttcacttc tcagctatcc aaggtgacgg attcaaaaca ttagaagaag gtcaagcagt
    19381 gactttcgac gttgaagaat cagaccgcgg cccacaagct gcaaacgttg taaaagctta
    19441 attttatctg taaatcaaat tgatggtaga catcaatttt tacgagagaa caaagcgagt
    19501 aacaaaaata cctgccacaa gagacaattt ctcttgtggc aggtattttt ttgtatgaaa
    19561 aagtaaataa atttaagagg ctatgaaaga gccaccttgt atcaaggaat aagaacaaac
    19621 aaaaatggct tctcatgttt ttcatttgtt ttctcttata ttcaggacaa ggtgctgatt
    19681 tcataacctc ttatttattc ttcatctggt gtcattacca ttggcaaaat cattggatgt
    19741 ctttctgtac gttcgaataa gaaaggctga agcgcgccag tcatcgcttc tttcaattta
    19801 tattctgaac agtcaggtcc attcaaacat tctcttaaag cgtggaataa gattcgctga
    19861 gcttcattga tcatttctcc tgattctcgc atgtagacaa atccacgaga gagaatatct
    19921 ggaccagcca agatttcttt tttcttgata tccactgtcg cgacagctaa tacaagacct
    19981 tcttctgaaa gcaggtgacg gtcacgtaaa acgacattac caatatcacc gatcccgcta
    20041 ccatcgatat acacatctcc agccacaaaa tgcccagcag gacgcgcaga atcgctagtt
    20101 aacgctaaaa catcgccatt ttccatgatg aagcagtttt ctgcaggaac acctacatct
    20161 tgtgctagag aagcatgtac tttcaacatg cggaattctc cgtggacagg aataaaatat
    20221 tttggcttca tcaaacgaag cattaatttt tgttcttctt gtccaccgtg accggaagta
    20281 tggatgttat tgatttttcc gtgaatcact tctgcaccag cttcagacag caaattgatc
    20341 aaatgattga cacttgttgt attaccagga ataggtgaac ttgaaaagat cactgtatcg
    20401 tctggctgga tttggatttg acggtgagta ccattcgcga tgcggctcaa cgcggccatc
    20461 gcttctcctt gagaacctgt acataggatc atcgtttcat tggcaggcaa gcgattgatt
    20521 tcgtgtgcat ctacaaatgt gccgtctgga actttgatat atcctaaacg ttgaccgttg
    20581 acgatggcat tttccatgct tcgtccgaaa acagcgattt tacgaccagt tgccacagcg
    20641 gcatctgctg cttgctgcaa acggaagatg ttagaagcaa agctggcaaa aatgatacgt
    20701 ccttcgattt ttgaaaagat cttcaaaata gaatgcccaa tcgttttttc tgatttagta
    20761 aatgttggaa tctcagcatt tgtactatca gataaaaggc acaagactcc ttcttccccg
    20821 atacgagcca ttttatgcaa gtttgctggt tcgccaacag gagtgaaatc gaatttgaag
    20881 tctccagtcg ctacgatatt tccagaaggt gttttcacca cgatccctaa tgcatctggg
    20941 atactatggg tcgttctgaa aaagctgaca gaagtctttc taaaacggat gatcgtatct
    21001 tcattgatct catggagctc tgcatctcga agcaagccat gctcatctaa tttgtttgtg
    21061 atcaacgcta atgcgagagg accagcatag ataggaatat ttgcttgttt cagcaagtag
    21121 ggtattccgc cgatgtggtc ttcgtgcccg tgagtaatca ccaaggcttt tactttgtgt
    21181 aagttttgta tgatgtagct gtagtcggga atgacatagt caattccaag taaatcgtct
    21241 tccgggaatt tgatcccggc atcaatcaaa atgatttcat cttggaattg taccccatac
    21301 gtatttttcc cgatttcacc taatccacca atagcaaaga cgcctgtctc gttatttttg
    21361 atggaaagtt tcatattaaa actccactaa tgagaaatca gcattttctt tttcgtactc
    21421 tagatgactt ccttctagaa gttgaacgaa ttcaatattg tatggggtat tttcttccac
    21481 acgttggcga gcgtctacat cgctttttgc ctcaatgtaa agagattttg tttcttcccg
    21541 tttagggttg cgtacttttg tttcttgata ataaacttta tagatcattg ttttgtctcc
    21601 tttatctgcg taaagcagtg atttagctag ttgatttttt tagacaacac aaaacgaggg
    21661 gacaagccac ctggtttttc atatgctttg actagaatac tggattcgag tcaaagagac
    21721 aagcctcgtt tttgcttatc tgttcggata ttttttcaac cgtaccttct gtataaaaaa
    21781 tatacaacaa ttaattgtta ttctagcata aaacagcaaa taagtataga aatcaactag
    21841 attgaaagaa aaaatcaatg tttatcatcc cgctataatc ataatgactt ttttatgaga
    21901 gaagtgttct attaataggc tcgttgtgct atcattttta gtaatgaaaa tgtaacaaaa
    21961 gattaaaagg agatgagaaa gtgaaactaa tgtggcatta taccatgcgt tataagaaat
    22021 tcttgttttt caatttcata tgcgtatttg gttttatttt gattgaatta ggacttccta
    22081 cgattttagc acgtatgatc gatgtaggaa tccgcaatga tgattttgat tatgtgaagc
    22141 aacaagggat atttatgatc gttattactg taatcggtat tattttgaat atcttgttag
    22201 gttattttgg ttcgaggatt acgactaata tcgtagcaga tattcgagat gatttgttcg
    22261 aaaagatcca aagttattcg catcaggaat acgaaaatct aggcgtatct tcattgatca
    22321 ctcgtacaac aaacgatgct tatcagatca tgttgttcct gcaaaatatt ttacgtatcg
    22381 gttttatggc accaatgatg ttcattgtca gtttgtacat ggtcatgcgt acaagtgtga
    22441 cactcagttt gtatgtagta ggtgcattac ctttactatt attggcagtc gtagcgattg
    22501 ctaaggtgtc tgaaccgtta tctaaaaagc aacaaaaaaa cttagataaa atcaacagta
    22561 tcttgcggga aaatctttca ggattgcgtg tgattcgtgc attcgtcaat gaaaagtttg
    22621 aagaaaaacg ttttgctgta gtcaatgaga attatgcttc tagttcaaaa agcttattcc
    22681 gtttgatggc tgttgcacaa ccgggttttt atttcttatt taatatcgta atggtattga
    22741 tcatctggag cggggcagtg caaatcgatg caggcgagct tcgtgtagga gatttgattg
    22801 cgtttatcga gtatatattc catgccttgt tctcattcat gttatttgct agtgtgttca
    22861 tgatgtatcc aagagcagct gtttcagctg gacgtatcca agaagcccta gatgccaaac
    22921 cttcgattgt agaagatcca aatggtatat ctgatacaaa aactaaaggg tatatcgaat
    22981 ttcaaaatgt aacatttgct tatccaggcc attcgcaaga gccagtgata cgaaatgtca
    23041 gttttacagc ctctccagga gagaccgttg cttttattgg aagtaccggt agcggaaaat
    23101 ctacgttgat ccaattgatc cctcgctttt atgatgtgag tgaaggaaag atcttgatcg
    23161 atggagtaga cgtgagagat tatcggctaa gcaagttgcg agataagatt gggtttattc
    23221 cgcaaaaagc gctgctattt actggaacga ttgcagataa tttgcgatac gggaaagaag
    23281 atgctacgca agaagaaatg gaacgtgcag ctgagattgc tcaagcttca gaattcattt
    23341 ctcgaaaacc agatggattt aatgaacatt tatcagaagg tggttccaat ttttcaggtg
    23401 gacaaaaaca acgattggca atagccagag ccatcatacg ccgtccggaa atctatattt
    23461 ttgatgacag cttttctgca ttagattatc aaacggatgc gaagttacgt gctcgtctga
    23521 aaaaagaaac gacagaatct actgtattga tcgtagcaca acgtgtggga acgatcatgc
    23581 atgcagataa gatcatcgta ttaaatgaag gagaagtcgt cggtatgggg acgcatcggg
    23641 agttgttgga aaaatgcccg atttattatg atattgccgc ttctcaatta tcaaaggagg
    23701 aattagcatg aaaaaaacct tttcttcctt aaaacgttta gctcgatata tcagaccgta
    23761 tcgtgagact tttcttttag tgatcctatt tacagttctg acagtggctt tcaacgtggc
    23821 tttgccatat gttacaggac ttcctactac agaaatcagc cgtaatattg ccaatggcga
    23881 atcaatcaat ttttcttata tattcaaatg tctcatttgg atcacagttg ttggaatagg
    23941 gtattgtatt tcgcaattac tttctggtgt tttgatgaca aatgtcgttc aaagtgccat
    24001 gcatgatttg agaagagaca tagaagaaaa aatcaatcgt cttccggttt catatttcga
    24061 taaaaaccag caaggaaata ttttgtctcg tgtgacgaat gatgtagatg cagtcagcgg
    24121 tgctttacaa caagctttta ttggtatcgt aaatgcgata ttgggaatta cgttggctgc
    24181 agcaatgatg ttttatatcc agccgatgat ggcgctgatc tctatgatca tgattccaac
    24241 atcgatctgg atctcaaaac gagtgatcaa tgcttcgcag aaatattttc aaagcatgca
    24301 gaattctctt ggtgagctaa acggctacgt ccaagaaaat atgacaggtt ttagtgtatt
    24361 gaaagtatat ggacgcgaaa aagaaacatt ggaaggattc aaaaaagtta atcacagctt
    24421 gaaatacttt gggtttcgtg cagcattcat ttctggcttg atgatgcctc tagttcaatt
    24481 aactgcctat gctacttata ttggtatggc tgttctagga agcttttatg ttattactgg
    24541 ggcgatcgtt gtcggtcaac tacaagcatt catccaatat atttggcaaa tcagtcagcc
    24601 aatggggaac gtgacacaat tgtcttcagt tttgcaaagt gcatcagcgg caacgaaacg
    24661 tgtattcgag attttagatg agccagaaga aaaagaaaac aaagtagatg cttcactgcc
    24721 agaaacgatt ttaggagatg tgacattcga tcatgtcgat tttgcatatg atccgaagaa
    24781 accattaatc caagatttga attttgaagt gaaagctggt caaaccgtag ctgtggtcgg
    24841 tccgaccggt gccggcaaga cgacattgat caatctattg atgcgttttt atgacgtaga
    24901 caaaggagca ataaggattg atggaatcga caccaaatca atgagtcgca gtgatgtgcg
    24961 ttcgctattt ggtatggtct tgcaagatgc atggctttat caaggaacga ttgcagataa
    25021 tatccgcttc ggaaagcttg atgcgacaga ttatgaagta gttgatgctg cgaaaacagc
    25081 aaacgtagat cattttatcc gaacgatgcc agatggatat gatatggcca tcaattctga
    25141 gggagacaat gtgtctttag gacaaaaaca gttgctgacg attgcccgag cagtggtctc
    25201 cgatcctaaa atcttgattt tagatgaagc aacaagttca gtagacaccc gtttagaagc
    25261 gttgattcaa aaagcaatgg atcgcgtgat ggagggacgg acaagtttcg tcatcgctca
    25321 ccgtttatcg acaatccgtg atgcagatct gattctagtg atggatcaag gtcagatcat
    25381 tgaaaaagga acgcatgaga gtttattggc acaaggtgga ttttatgaaa aactgtataa
    25441 cagccaattt gctgaggaag cagaataagt catataagaa taaaacagtt taaaacttat
    25501 tttttgttct ctattcttga taatggtaaa gtaaagttat caagaaaagt gaggacacgg
    25561 agatggaaca gtttttaaat gaaaaaatta ctgaagccaa aagagaagtt gatcgtttaa
    25621 cgcacgtctg tcagacgaag ccttttgatt cagctgattt ttttgaaaat gagtttcaaa
    25681 tcgaacgctt ggcagcacaa atagaagtgt acgaattaat tttaaaaaaa gtaatagaag
    25741 aaaatataga tgtacaataa aaacaggtaa aacgtcagtt accagatact gtaaatagac
    25801 atctggcctg agcaggtttt acctgttttt tttcttatga aataaatata tgctatgata
    25861 cctttattca ataggattag tttctacaaa aaatgaaagg agaaaaataa atatggaacg
    25921 gtttatagca tcaaatgcca cagtgattgg tgatgtgaca ttgtcagaag atgtgacgat
    25981 ctggtatcaa gctgttttac gcggggacag taattggata aaaataggac aaagaacgaa
    26041 tatccaagat ggtacaatca tccatgtcga tcatgatgca cctgtagata ttgcggagaa
    26101 tgtgacggta ggacatcagt gcatgctaca tggctgtaca atcgaaaaag gggcgctgat
    26161 cgggatgggg accacgatat tgaatcatgc ggttattggt gagaatagtt tgattggtgc
    26221 tggatcatta gtaacagaag gaaaagttat tcctcctaat gtcttggctt ttggtcgccc
    26281 agctaaagtg atccgtcctt tgacaaaaga agaaatacaa aaaaacaaag aaaacatcca
    26341 gcattatgtc gagataggtc ggatgcatgc gcaaggaaaa ttcaaggagc aaagataggc
    26401 agtagcatcc ataaaataaa aaaggatggt cagtttgagt tgaccatcct tttttatttg
    26461 gtttagataa gagagcttaa aaaagcttcc tttgaaacag attaatttaa tgaagaactg
    26521 ttttccatga cttcatcgat caatccatat gcttttgctt gttcagctgt catatagttg
    26581 tcacgatctg tatctttttc aatcacttca agcggttgtc cagtgcgttc agctaaaatt
    26641 ttgtttaagc gttcgcgtgt ttgcaaaata tggcgtgccg caatttcgat ttcagttgct
    26701 tgcccttgag cgccgccaag aggttgatgg atcatgatct cagcatttgg taaagcaaat
    26761 cgtttacctt ttgtaccagc tgtcagcaag aagcttccca ttgaagcagc catacccata
    26821 acaattgttt ggacatctgc tttgacaaag ttcattgtgt cgtaaatagc catacctgct
    26881 gtaactgaac caccaggaga attgatataa agatagatat ctttttcaga atcttgagcg
    26941 tctaagaaaa gcaattgggc gatgatcgaa ttagcaagat catctgttac ttggccgctt
    27001 aacatgatga tacggtcttt caataaacgt gagtagatat cgtaagctct ttcacctcgt
    27061 gaagattgtt caatgactgt aggaattaaa ttcataatag agttcctcct tgatttcgtt
    27121 tagaaatagt gtaccataat ttagtttcta atacctaaaa tgtagtatac atttttggtc
    27181 aaaaaaggtc aaataaaaaa cttaggcaaa ttagtcgcat aaacccgctt tatggtatga
    27241 taagcagaga aacaaaacaa ggaggaacaa cagattgata gttggtatta ctggccttat
    27301 cggagtgctg acaggtgctg tagttgtcta tatcggcatg atactgcaaa tgcgtcactt
    27361 ggaaaataaa gaacagcgta gacgtgaatt ggaattgaaa gtcaaagaaa tcgagacgct
    27421 gaatcaatta aataaaaaga tcaacgagat cttgcaaaaa cggacaacgc tgatggaaga
    27481 atatgtcagc tttgatgcat ttgatgattg ttatatcacg attgacgatt ttgcgtatct
    27541 ccaatcattt tcagcgcaaa atagttttta tctgccaaat tatattttag acgagctatt
    27601 taagaatatc tcccatcgaa aagtgatttt atctccagat gaaacaatga agattggtgg
    27661 atatgcttat aaaggcggac gcgtgatttt agagaatttt acggatagtc tgactgaaat
    27721 gatcaatgaa aaaaaagctc agttgaagac tatgacgcgc cagccgattg gtttcttctc
    27781 gaaagatgat gtttggagct gatattttga agcattgccg ttgcacatga gagcgtggta
    27841 atgctttctt tttttcttaa tgatacaata gaactgaacg gagtgaagaa agatgttaca
    27901 aatctatgcc catcgcggaa gcagcggcac ccacccagaa aatacgcttc ctgcatttgc
    27961 agaagctgta cgagtaggtg cagatggtat cgaactagat gtccatctaa gcaaagatgg
    28021 ttatttgatc gttatgcacg acgaagaagt ggaccggacg actaacggta aagggctgat
    28081 tcgagaaaaa acattggaag aactcaaaaa attgaatgca gggggcaaat tcagcaaaga
    28141 atttccagca gctaaagttc ctgaattgag agaagtgtta ggactgctcc tccaaaaaaa
    28201 ttaccgaggg atgctaatga ttgaaattaa aactgatcag tatgaatatc ccggtatcga
    28261 agaaaaatta gccgaaacat taaaaagtag agattggcct tttcaacatg catattgcag
    28321 ctttcatttg gattctttgg aaaaaatcgc attactggaa ccggagatac agctggactt
    28381 attgatgagt acttctgata aaaagatcca actagcagca gaaacccctt atatagaagg
    28441 agtacatcca aaaaatactt ggttgttcga acatccagtc caggcagctg attttccaaa
    28501 agcaattcgt gcatggacga ttaacgatga agaagaaatg gccaaatgtc aagaactcaa
    28561 aatagatgga ataataactg attttccgga gaaagctctt cggtggaaac aatcaacgaa
    28621 aagtaggtaa aatatggaaa aagtattagt gatcgtcggt ccgacagcag tcggtaaaac
    28681 agcgctaagt gtggaactcg cacaactatt tggaggagag atcatcagtg gtgattcatt
    28741 gcaaatctat aaaaatctgg atatcggtac agcaaaaatc aaagaagaag agatgaataa
    28801 tgttccgcat catttgattg atgtgatcga acctactcaa cgttactcag ccgttgattt
    28861 tcagcattca ggtcgacaat tgattaccga gattgcgaac cgagggcacc tacctatcat
    28921 agctggtggt acgggtctct atatccaatc ccttctatat gattatcagt taggagcgac
    28981 agaagaagcg aatcctgaga ttagaaaaaa gtatgaaacg ttagctgaaa caattggttc
    29041 ccaagcatta tggaatcagt tgaaagaaaa agacccggca gctgctgaga aaatccactg
    29101 gaacaatcaa cgcaaagtga taagagctct ggaagtttta gagacgacag gacatagtat
    29161 cacggcacct aaagaagagc caaagcaatt gtatgattac ctcatgattg gattagatac
    29221 gaagcgtagt attctttatg aacgaatcaa tcaaagagtc gatttgatgc tagcagaagg
    29281 tttagtagca gaagcgcgta aggtgtatga gcttggagaa gtccaggcca gtcaaggtat
    29341 tggatataaa gaatttttcc cttattttga agggaaagaa acattggaag aagcaacaga
    29401 acaagtcaaa ctacattcga gaagatacgc gaaacgtcag ttgacgtggt ttcgtaaccg
    29461 tttggatgca cagtggtttg atcttataag tgagccaaat caaaaaagtc agattgagca
    29521 aacaatcaaa aaatggctgg aggcggaata aaatggaaga aaaagtgatc atcgtagggg
    29581 tagaaaccga agcaaaccag cgttactttt cagagtcaat ggaagagcta gttcagctga
    29641 cgaataccgc atcaggagaa gtcgtattca caattacaca aaagagacca caagtggatc
    29701 ggcaaacgat tatcggtaaa gggaaattac aagaattagt tcaacaagcg gatgcgcatg
    29761 aagcggattt gatcatcttt aatcatgagc taactccgag acagagccaa ctgattaccg
    29821 atgctgttgg tcttccggta attgatcgcg tgcagcttat cttagatatt tttgcgatgc
    29881 gtgctcgttc gaaagaaggg aagctgcagg ttgaacttgc tcagttagaa tacctgctgc
    29941 ctcgtttagt agggcaaggg aaaacattat ctcgtcttgg cggtgggatc ggaacaagag
    30001 ggccaggtga aacaaagtta gaaacagacc gccgacatat acggaataaa atttttgctg
    30061 ttaagaaaga attgaaagaa gtagaagctc atcgagagcg caatcgccaa aaaagaaaaa
    30121 atagcgagat ttttcagatt ggacttattg gctacacgaa tgccgggaag tcaactatct
    30181 tgaatctatt gacacaagcg gatacttatt cgaaagatca attgtttgca acgttagatc
    30241 cattgactaa gagatggcgt tttgctgaag gatttgagat tactgtaacg gataccgtcg
    30301 ggttcatcca agacctgcct acgcaactga tcgatgcttt ccattctaca ttagaagaaa
    30361 gtcaaaatat ggatcttttg ctacatgtag tcgatgctag ttctccagat cgtattttac
    30421 aggagcagac tgtcttaaaa ttgatggatg aactgaatat gaaagaaatg ccgatactga
    30481 ctgtttacaa taaggcagac cagatcgacc cagcgatgtt cacgcctacg ctattcccaa
    30541 atgtattgat ttctactcag acgaaagaag gtaagatggc gttgatcgaa gcaatcaaac
    30601 gtcagctgat ggaactaatg accccttata cactttctgt gccaagcaac gaaggacaga
    30661 aattaagcga gttgagaaga caaacaatgg ttttggacga agcgtacatg gaagaaacaa
    30721 acgaatatga agttaaaggg tttgcaaaaa ccaattcaaa atggttaaga aaaaaccaat
    30781 aaaattttat ttttttaa
//
LOCUS       DAZKLM010000025        29370 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3764
            SAMEA115706913-rid22582563.denovo.025, whole genome shotgun
            sequence.
ACCESSION   DAZKLM010000025 DAZKLM010000000
VERSION     DAZKLM010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706913
            Sequence Read Archive: ERR13196647
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 29370)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29370)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287823.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287823.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,004
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,849
            CDSs (with protein)               :: 2,849
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 41 of 96
            Pseudo Genes (incomplete)         :: 52 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 15 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29370
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706913-rid22582563.denovo.025"
                     /isolate="VRE3764"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(424..1368)
                     /locus_tag="ACG842_001047"
     CDS             complement(424..1368)
                     /locus_tag="ACG842_001047"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004459 - L-lactate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HFQ0247608.1"
                     /translation="MKKTSRKVVIVGTGFVGTSIAYAMINQGISNELVLIDVNQEKAE
                     GEALDLLDGMAWGDENVAVWSGGYEECKDADIVVITAGINQKPGQSRLDLVKTNASIM
                     RQIVKEIMGSGFDGIIVVASNPVDILTYIAWNESGLPTSRVIGTGTTLDTTRFRKEIA
                     LKLKVDPRSVHGYILGEHGDSEVAAWSHTTVGGKPVFEIVEKDHRIAQDELDVIADKV
                     RNAAYEIIDRKKATYYGIGMSTARIVKAILNNEQAVLPVSAYLTGEYDEKDIFTGVPS
                     IVDENGVREVVELSINEEEKAMFSKSTSALREVLNTVL"
     gene            complement(1664..2608)
                     /locus_tag="ACG842_001048"
     CDS             complement(1664..2608)
                     /locus_tag="ACG842_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFQ0247609.1"
                     /translation="MRNEESIETLQREQGMNINDLNLAYHLQTPPKIHRGDLFQQGNI
                     HISKHRRYAEVPEHTHDFVEINYMYSGTCLQIINGERIEMEESDVVIIDKEATQRIEY
                     TGQEDILINILLKEETLSTEILNHLASTNNLLSSFLINASKEKGEHTQFIHFRGGKTS
                     RLTHLIENMLLIYFDTYSHKIRSLNLYTSLLLIELTHLLEQQSFSSADGCAENEMLRV
                     LDYIDKHFTSCTLKETAAHFGYNPVYLSNKLKMYFDASFQELVLKKKFDFALELIQET
                     SFSLEEIANRIGYKQTTSLFKLFKKYSTCTPNEWRNKH"
     gene            2828..4042
                     /locus_tag="ACG842_001049"
     CDS             2828..4042
                     /locus_tag="ACG842_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFQ0247610.1"
                     /translation="MLMNKKMMKFSLLSVSLILTSAGAVTSAIPSMSATYATRSLSAV
                     ELLTTAPSFMVTIFVLISSFIARKIGQKQTVIIGLVIASICGILPLFSQSYSIVLASR
                     AGLGIGFGLINSLAVSMIAAFYHGDERASMIGFQSAFQGFGAALMTFVAGQLVQFGWQ
                     YTFLVYAISLPILVLFVLFVPTPETTELQSHGTAPTRQVIKTNPKMISYGIFLLLVIV
                     VYNAVSIKLATVLTTYSLGNETNAANILTIMQLASMAAGVCFGWLQTKAHRYMLTLSL
                     LLMGIGFVLIATVINLYVIGFGAILTGISFSLFIPYLFNQVSIQAPKYAESFNTSILL
                     VGANLGTFIAPYGLLFLSFASLGSKTLAVFVNGSILLLISALISLLVVHRTNRKTVIR
                     VDEEIKEQLTTK"
     gene            4064..6079
                     /locus_tag="ACG842_001050"
     CDS             4064..6079
                     /locus_tag="ACG842_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HFQ0247611.1"
                     /translation="MTNEKHKDRWLWYPGDFEIRHGLLQNFQREERGFDWPAYWYMDD
                     CHRNVKFKRYYFLDQPSMFKVTIQGVGYVEINGQKHPCGKWLTCPAGKAKIRIFVGHT
                     SGLPAMFIEGDEVKSDIGWTASNFIEEYPAGWSPLYVDIAKDPNQIYYQKECVHPVTE
                     TEIGGGILFDFGRAVNGCVELISLDDHIESITLCYGESDVEAMDIDYCYYKQIGMTRK
                     MKARKRAFRYLFIPEVSTGMVQLQVFHEFLSLSKQAAFSSDSSLLNQIWSVSEEAFKL
                     CSDLFFIDGIKRDRWIWSGDAYQSFMINQYLFFDEEINKRTILALRGQNEIKQHMNTI
                     VDYSILWLIGIENHYMMTKDYEFLRIVYPKMQSLMRYLMEQTNELGFIYGRAKDWIFI
                     DWSEMDKEGTLAAEQVLLLKAYHSMITCGDILAETYGENENYHTHLYQEKYQLLKQNL
                     FDYFWDEELGAFIDCYESGRRNVTRHANIFAVLFDFANKDQQQSILKNVLLNDTITQI
                     TTPYFKFYEQDALCKLGQTNIVYQTILDYWGGMLSHDAVTFWEEYDPTQTGNEKYSMY
                     GDPYGKSLCHAWGASPIYLLGRYFIGLYPTKPGYEEFMIQPNLDSFETLNCTLPIKDG
                     KISLEKTKDKIVITSDRTGGTVKMNGKSYPLLAKQPLVLTSYSKINA"
     gene            complement(6162..7535)
                     /locus_tag="ACG842_001051"
     CDS             complement(6162..7535)
                     /locus_tag="ACG842_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HFQ0247612.1"
                     /translation="MDKLLNLFVSIVSQPSILVSLIALLGLVLQKKKFSEVIQGTIKT
                     FVGFLVLIGGAGLISNSLTPFASMFQTALHTQGVVPSNEAVVAIALQQYGTPTALIML
                     VGMIVNILLARFTRYKFIFLTGQAMLYVSCLTAVILVSAGFTANLWMILLGGVFEGTL
                     LTITPALCQPFMKNITGNNQVALGHTGNIGYASSGWIGKFFGNKDRSTEDIKIPKSFS
                     FLRDSTVSIMLLMSIVYVALALLAGPGFVERELSNGDNAVIFALIQAGTFTAGFVIVL
                     QGVRMVLAEIVPAFQGIAKKLVPDSKPALDVPIVFPYAPNAVLIGFFVSFFVGTLSMF
                     AMIGLQTTVIIPGVVGHFFCGAASGVFGNSTGGRRGAVLGSAFNSLLISWLPLFILPV
                     LGDLKIASSTFADTDYLVPGLVLGKLGEYGQIALTVGIIAFFLLVVLSSFLFAGKRTN
                     IVSKEQN"
     gene            complement(7561..7845)
                     /locus_tag="ACG842_001052"
     CDS             complement(7561..7845)
                     /locus_tag="ACG842_001052"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFQ0247613.1"
                     /translation="MKIFAVCQSGLGTSFMVEMNIKQALADLGADVDEFEVSHTDVGS
                     VSSDMADYFFIEHSLKDTLTSIPDEKLVPLQSIIDSEEVKEKVTKILSKE"
     gene            complement(7847..9862)
                     /locus_tag="ACG842_001053"
     CDS             complement(7847..9862)
                     /locus_tag="ACG842_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFQ0247614.1"
                     /translation="MNYKISYLLKTPREISLEKAAEIIEVNTSEAIKQLTPFAEYVDD
                     TRIRIKKFRAIDWAEKFLDITRFEAVYTEQERRDLLYLMVYSTATSVSVFHFQDFLQV
                     SKGTVLSDIKKIRNELEVEDCQLSYTRKTGFRLLGSEAILRREAKNRTARLMQSLAGR
                     FGLTYLTATLSIDYYAKVRDLLDKTISLSDLRFAPNRMDELAYYLSLTTIRIGKTSME
                     LVKDAELLASLAVTDISRQVLTGLGYSEHQNSEIIFFSLCLASIVEGDVQEPALDFLL
                     ECSSEVIHRMEFLMAVEFASFRELLMNVFYHLVPAYFRISYSFYLPNVMIDQIKHQYA
                     SIYEMTRKALLPLERRIGKSIPEEEIGFFTILFGGEIRKADAEERNRKIRAVIVCPSG
                     ISSSLILKSELQQLFPMIFFTETNSADRLHEVDETSYDIIFSTVPLTLTDKKKSLYVL
                     HPIMTDLEKNQLINQVQRDWLIPGFSMPSAQELLSALLPYIELKEGVDEEKLYQVLNR
                     KMNKLLEKKEDQRPMLSELLTEEMIQLSDQPKNWQEAITFAAQPLLSENKIEESYIKA
                     MIERVEQYGAFIHIGDHIALPHARPEDGVNEVGMSLLKLQQPVDLLDNPAHPISLFIC
                     LAAIDNEAHLRALANLTKLLSNKQNLQDLLHASTKEEILAIIRKGDE"
     gene            complement(10041..10346)
                     /locus_tag="ACG842_001054"
     CDS             complement(10041..10346)
                     /locus_tag="ACG842_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA processing protein"
                     /protein_id="HFQ0247615.1"
                     /translation="MTNYDGEKHKRLRGSMAPKTEEEVALAGTNSGETEDKEESLAGT
                     NSGETENKETALSGSNSVEEETKPNEPNHFEKDIEKAVEDVKEKFEELTSKNKHDHH"
     gene            complement(10435..11465)
                     /locus_tag="ACG842_001055"
                     /pseudo
     CDS             complement(10435..11465)
                     /locus_tag="ACG842_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306737.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
     gene            11672..12547
                     /locus_tag="ACG842_001056"
     CDS             11672..12547
                     /locus_tag="ACG842_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFQ0247616.1"
                     /translation="MLIEEKLTKQELFTTTEKRIVDYIRRNIEAAVYMTIEELAKATY
                     TSHSAIIRLCKKMGFSGFKEFRFELGREVHQLIAQFNQTIDPNFPFSSEDSTQQIAKK
                     MADLSIQSIKKAQLQIEHEDLDQIGKVLNKAQRIFLFAKGDSQITARKFQNKMVKLNK
                     FLIMAEEYSDSSWNAANLTSEDCAIFISYSGRIHHYERIMTYLKHVEAPTLLITGNQH
                     SEMAKQADMCLVISQEEYDFAKVATFSSQIAFDYVLNTLFSVIYSQNFEENIRNLKNK
                     QELMQKGLLKEYRKT"
     gene            complement(12699..13541)
                     /locus_tag="ACG842_001057"
     CDS             complement(12699..13541)
                     /locus_tag="ACG842_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFQ0247617.1"
                     /translation="MKPKKRKLLDLLMLFLLLSGIGILAYPFVSDALNNYLDQQIISH
                     YQQQAVKENEEVMAKIQENMTKKNQQLAKEGGNPGADPFTKKKEPAKKDRTYFEKHTI
                     GILTIPEINVHLPIFDETNTVLLEKGASLLEGTSYPTGGENTHAVISSHRGLPQAKLF
                     TDLPKLTLGDSFYIEINGRTLAYQVDQIKTVEPTDTKDLRIEKGQDFVTLLTCTPYMV
                     NSHRLLVRGHRVPYHAKEVQKEVQKVSENKRFFLYGLLAACILIFLLLIYLFRQSFKI
                     RNRS"
     gene            complement(13633..15510)
                     /locus_tag="ACG842_001058"
     CDS             complement(13633..15510)
                     /locus_tag="ACG842_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HFQ0247618.1"
                     /translation="MKNHKKINVMLGVLFLILPLLTNSFGAKKVFAEETAAQVILHKK
                     KMTDLPDPLIQNSGKEMSEFDQYQGLADISFSVYNVTQEFYAQRDKGASVDAAKQAVQ
                     SLTPGTPVASGTTDADGNVTLSLPKKQNGKDAVYTIKEEPKDGVSAAANMVLAFPVYE
                     MIKQADGSYKYGTEELDTIHLYPKNTVGNDGTLKVTKIGTAENEALNGAEFIISKEEG
                     TPSVKKYIQSVTDGLYTWTTDQTKAKHFITGHSYDIGNNDFAEASIEKGQLIVNHLEV
                     GKYNLEEVKAPDNAEMIEKQTITPFEILANSQTPVEKTIKNDTSKVDKTTPQLNGKDV
                     AIGEKIQYEISVNIPLGIADKEGTQNKYTTFKLIDTHDAALTFDNDSSGTYAYALYDG
                     NKEIDPVNYSVTEQTDGFTVSVDPNYIPSLTPGGTLKFVYYMHLNEKADPTKGFSNQA
                     NVDNGHTNDQTPPSVDVVTGGKRFVKVDGDVTSDQTLAGAEFVVRDQDSDTAKYLSID
                     PSTKAVSWVSAKESATVFTTTSNGLIDVTGLKYGTYYLEETKAPEKYVPLTNRVAFTI
                     DEQSYVTAGQLISPEKIPNKHKGTLPSTGGKGIYVYIGAGVVLLLIAGLYFARRKHSQ
                     I"
     gene            complement(15507..16928)
                     /locus_tag="ACG842_001059"
     CDS             complement(15507..16928)
                     /locus_tag="ACG842_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HFQ0247619.1"
                     /translation="MKKLGWLSMCLFLLLFKPAFTQVATETETEMVQITLHKLLFPNG
                     QLPKNHPNDGQEKALLQTYRGLNGVTFQVYDVTDSFYHLREKGKTVEEAQAEIAKNGA
                     SSGMFTAEATTTTLNNEDGIASFSLAAKDQEKRDKAYLFIESKVPEVVKEKAENMVVV
                     LPVHGQNNQKLSTIHLYPKNEENDYPDPPFEKVLEEPRNDFTIGEKITYSLHTTIPVN
                     ILDYQKFELSDSADEALTFLPNSLTISSNGEKLTEGFVIHKKPHGFDVLFSIPSLEKY
                     AGKKLTISYQMQLSSTAQANKEINNNGTLDFGFGVSTKKVSVYTGSKQFVKIETNKPD
                     KRLAGAVFLIKNKAGNYLQQTANGYKWTKNESDALHLISDKNGAFSISGLKTGSYRLK
                     EIEAPSGYILSETEIPFTISTFLSEDKEADSILKVVNKKENSRPFLPKTNETKNTLLG
                     VVGMVFASFAIWLFIKKRTGVKK"
     gene            complement(16931..20320)
                     /locus_tag="ACG842_001060"
     CDS             complement(16931..20320)
                     /locus_tag="ACG842_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HFQ0247620.1"
                     /translation="MTTTGKKLKVIFMLIILSLSNFVPLSAIADTTDDPTVLETISAE
                     VISDQSGKKALNIKLNANNTSAEKIEKEIGLVENYLSDVERKEGDGYAYQVNSGKITL
                     EISSNTKQTIDLSFPIDPALYHSQANKLIVDNKEYDIIDETENKKDTDVSVPKPDEIE
                     EESSKENENSVSPFTLPTLSLPAVSVPSNQTIPTEYTTDDQGTYPKASWQPTGNTNVL
                     DHQGNKNGTNQWDGINSWNGDPNDRTHSYIEYGGTGNQADYAIRKYAKETSTPGLFDV
                     YLNARGNVQKDITPLDLVLVVDWSGSMNDNNRIGEVKIGVDRFVDTLADSGITDKINM
                     GYVGYSSEGYSYSNGAVQMGSFDSVKNQVKSITPSRTNGGTFTQKALRDAGSMLSVPN
                     GHKKVIVLLTDGVPTFSYKVQRVHAQSSSNYYGTQFSNTQDRPGNTSLISRIYDAPDQ
                     NNLSRRIDSTFIATIGEAMALKERGIEIHGLGIQLQSDPAAGLSKAEVESRMRQMVSS
                     DEKGDLYYESADHATDISEYLAKKAVQISATVSNGQINDPIAEPFIYQPGTLSVKSVG
                     TSPTTVTPSISIEGNTIKSNQIYLGKDQEIQIHYQVRIQTENEDFHPNFWYQMNGRTT
                     FQPNIDTNELAEFGIPSAKAPGVSLHIKKLWEEFDNNLADRPDQVTFEIQREHTTNAA
                     AWKNGYIRIIKPAKDTTNTWERADIDKLSANSGESYQEILSLPQYNNQGQAFSYQTIK
                     ELPVPGYDSQQIDAMTWKNTKQFTPLNLKITKNSSTGEKDLIGAVFKLTGDSIDTLLT
                     DHGDGTYSLPENVKLQKEMTYTLTETKAPEGHGLSKKTTWEIKIASDGTVTIDGKTVT
                     TSDDTIQLTIENPFVEVPVAVRKYAMQGTDKEINLKGAAFSLQKKEANGTYQPIDSQT
                     TNEKGLASFDSLTPGKYRVVETAGPAGYDTSPGNYEFQIDKYGKIIYTGKNTEMTNNV
                     WTLTHQNRLKAFDLTVHKKEDNGQTLKGAKFRLQGPEMDLESPKDGQETDTFLFENLK
                     PGTYTLTETFTPEGYQGLKEPVTIVIHEDGSIQVDGQDHESVLSPGAKNNQISLDITN
                     QAKVPLPETGGIGRLGIYLVGMIGCAFSIWYLFLKKERGGS"
     gene            20577..21968
                     /locus_tag="ACG842_001061"
     CDS             20577..21968
                     /locus_tag="ACG842_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0247621.1"
                     /translation="MEKIIRILSISDKWMTKKELAKRIGSSESTFVRYIEEIKIRWGT
                     DVTIQSSQKLGYRLEHLNVPAYLNVLMEMSQSSTNSRLLNEIIVNPGKTIEYYCEKIS
                     ISRSSFTRKLKQCNQILESYLLKVIVDQGFQLTSLKSELQLRIFVTFFFLSYYGRRTL
                     PFPIEKKKIKEIMVKNNCQMNLFQEESPYEQSFFIMYFVVHLMRESQGTPFEWKAGDS
                     YVQLINIDKKDFLILKNLLGKLKYSSYIKVVNYFVNTIFSEFSEKEKNEISEQIRKNM
                     SYCSFSSADCKEKNEEYVVQLLTQIYFFSRIFPYDTTHLTIRATYFAQQMQHRQIRLY
                     AGFRAELQFYSTLLNQNLIYYLSSYIFWMLIGNPEIHHYTSDKKILIISDLSLRHSQY
                     IEEYISDILAVHKIHSETTAITEDQLSKYNLLEYDLIVTNQPILNAHVPHILIDDSVS
                     FANEEELIRLFEL"
     gene            complement(22097..23371)
                     /locus_tag="ACG842_001062"
     CDS             complement(22097..23371)
                     /locus_tag="ACG842_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element IS1476 family transposase"
                     /protein_id="HFQ0247622.1"
                     /translation="MDNHTRKLLGLTDKHLFFDEEWLIEKEYKGVQAQFIKGRLDDSP
                     SHCEHCGSIRIIRNGSYTTRTQMLKVKEKLTILELKRTRFLCHDCGRTFSAKTDLVDE
                     HHQLTKELKQAILMELYENQSRKLIAKKYFVSDGTVTRILREATKHYQPRMNFLPTVL
                     CMDEFKSMKSVSGSMSFICVDGTTNQLFTILEDRRLYKLTQYFLRFPRKARLKVKYLV
                     MDMNASYCQLLKTVFPNAEIVTDRFHIVQHINRSFNQLRVQIMNRFRTSHSENQKKYR
                     RLKRYWKLLLKDSTTLEPLKRHYHRLFKRPISQTEIVDELLSYNEELRTAYHFCQLLR
                     YYFVKRDTEGFQETLKTISSTLPQSFKKKFTIFHRYQSGISNAFKVPHSNGVTEGLNN
                     KIKLIKRIAFGYRNFYNFRARIYLQQGLIFEN"
     gene            complement(23550..24197)
                     /locus_tag="ACG842_001063"
     CDS             complement(23550..24197)
                     /locus_tag="ACG842_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFQ0247623.1"
                     /translation="MTMKGYPRIIRDKGEMWMPKETFFNLSNAKKQRIDDLLLEIFCS
                     QHISQVKVSEIVDKMGMSRGAFYKYFQDLEDAYLYTIKSSSHIVHQDILKFISENKQH
                     FFLGIEKYLEWCCDLDHESIYWQRLYLLTQRNDQQTTKRRELSADSPMNEKWLELLRI
                     NHFSIQSSEEAVSFLYFSMTLVMNTLTDLIANNWTKEELLKDFRYKSRWIAEGIQ"
     gene            complement(24205..24891)
                     /locus_tag="ACG842_001064"
     CDS             complement(24205..24891)
                     /locus_tag="ACG842_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002366355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0247624.1"
                     /translation="MKKILTMKKVTKTFGSGHTKVSALKAIDFYVKEGEFVSIIGPSG
                     SGKSTFLTISGGLQTPTSGEIVINGHSLTELPEKKRADLRFKEIGFILQSSNLIPFLT
                     VKEQFELVDQVTSKKEDSTKIDSLLQSLDIQSLKNSYPKDLSGGERQRVAIARALYNE
                     PSLILADEPTASLDTDHAYEVVKLLVKEAHEKQKATIMVTHDVRMIQWSDRVYRMEDG
                     YLTEEEKKKE"
     gene            complement(24897..25970)
                     /locus_tag="ACG842_001065"
     CDS             complement(24897..25970)
                     /locus_tag="ACG842_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0247625.1"
                     /translation="MFLAIKEIRYSKLRYGLIVGIMFLIAYVVFMLSGLANGLSEEFK
                     KAIDDWDAQEIVLSEDANQVFAASQLTRGDLKYIEGGEKAPIGLYSGAIKGKNKENVT
                     VFGTTKEAFLLPKLTKGHEFKTKNEIIISQNLADSGYKIGDTIKIGSYDDPLTIVGIF
                     PETYYTVSPVIYASLDTWTALKYGQQPFASEEEQPINGIVIKDSAKISKEKASKGLQL
                     LTIGTFIESIPGYSAQNMTLNAMIYFLFLVVAAVVGIFMYVMTLQKTAIFGVMKAQGI
                     KNFFIAKSLVAQSFIVGVVGVLLALLFAYLTSLILPSAMPFAVFWSQWLLYSGILIIV
                     AMLGGLFSIRTITKVDPITAIGG"
     gene            26192..26866
                     /locus_tag="ACG842_001066"
     CDS             26192..26866
                     /locus_tag="ACG842_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFQ0247626.1"
                     /translation="MIKLLVVEDDKVLSNNIKEILAAIGEITQVYDGSEGLFELESGV
                     YDLAILDLMMPEMNGYEVLAKIRKEKIQTPVLILTAKDSLEDKIEGFERGADDYLTKP
                     FYREELIMRVKALLKRSLGLFDENVLKYKDISCDLKSNEVLYKEQVLPIQGKEFELLV
                     YFLQNKGIILTKEQIFDRIWGFDSDTTITVIEVYMSNLRKHLRSTGLDKEIKTLRNVG
                     YILQRV"
     gene            26871..28211
                     /locus_tag="ACG842_001067"
     CDS             26871..28211
                     /locus_tag="ACG842_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HFQ0247627.1"
                     /translation="MNGDITRKQRSRFFATNVAAFAFIFFLLGFITLQILNQSAYRET
                     DLSLEKINFDSRTVQLEIQRYQQGDPFLNPTQTEQNRTNMEQPLMEPSNDRFNTQVIL
                     WSKNGEILNKAAIGGRFNQIKELKMDTKNLNTIQNIELNEDSGSSALSFRSITKKAET
                     NQDGVAYIQILENTNQIKNSLATFRTIMILCMIVFWILSIGISYYLSSLSMRPIILSW
                     RRQKEFVENASHELRTPLTIIQNSLEHLFTKPNHTIIEESESIAQALSETRRLTGLTS
                     DLLTIARNDSDQQLLSKQMINTQEYIEQLVKPFQEMAIIDGKEFILHNDATLEVPIDE
                     KKIHQVLVILLDNALKYTNPEDKIILDSSVNHKEWLIHVKNTGPSISEEDQARIFDRF
                     YREDQSRAKETGGYGLGLAIAKQVIEQHHGQISVSNYLPQGVDFKVQLPLKPKK"
     gene            complement(28360..29346)
                     /locus_tag="ACG842_001068"
     CDS             complement(28360..29346)
                     /locus_tag="ACG842_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2382 domain-containing protein"
                     /protein_id="HFQ0247628.1"
                     /translation="MEEKNLTVIGSYSTREEALSVIERLRNEGYERDDIVIYTTDEAA
                     SRLGFDGLSGIDVETDENRMDGEEDRSLWEKIKDTFSFDTYDSETATPENDPLYQYRG
                     DISDGKFVITVKGYRQPETTEDTMDTQDTVSPASTMGTGLETGTGTDPMDVGGTTGFQ
                     NTTDPLNMTPGTDPMDVPGETDTMGTSKTEDIDRKPNLDDDTIQLKEEKLDVNTHDVT
                     TGEVDIHKHVVNDTETVEVPVKREEIVIERKPVTDQSSQGTDEKLEDDTITIPIKEEQ
                     VDVSKHTVIREEVGIHKEEHEDVEKVTEDVSREELDIDTSGDVHIEDRNKKS"
BASE COUNT         8562 a         5554 c         5359 g         9895 t
ORIGIN      
        1 aaaaatgata aacctctctc gaacggtttt ttatcaaaaa tcgtcatttt tccatcaaaa
       61 tctgcttatg caaaattata aaaaaatttt gcagtttttc ttactttttt tgtcgtttaa
      121 ttgaatgata aaaaccagtt tctttattca gttaatcaaa tatgaacgaa ttagatatga
      181 atcattgtat ttgccgttgc ggaaggattt tttaattaac cacagataaa cattgataac
      241 gattcgctta ttgtaatcaa cagattaaat ttcatatgtt catatgtacg gatacatttt
      301 cggaccgatt aattttaacc attctattta tttgcttacc tattagatat caataaaaaa
      361 ctgcttaaca gctatttcag ctgttaagca gttttaggat ttttagttca tattcattgt
      421 ttcttataat acagtgttca atacttctct taaagcactt gttgatttgc tgaacattgc
      481 tttttcttct tcgttgattg ataattcaac aacttcacga acaccatttt cgtcaacgat
      541 tgatggcaca cctgtaaaga tatctttttc gtcgtattca ccagttaaat aagcagatac
      601 aggcaataca gcttgttcgt tattcaagat agcttttacg atacgtgcag tactcatacc
      661 aataccatag taagttgctt ttttacgatc aatgatctca taagcagcat tacggacttt
      721 atcagcaatc acatctaatt catcttgggc aatacgatgg tctttttcta caatttcaaa
      781 cactggttta ccaccgacag ttgtatgtga ccatgcagca acttctgaat caccatgttc
      841 acctaagata tagccgtgga cgctgcgtgg gtcaactttt aatttcaatg cgatttcttt
      901 acggaaacga gttgtatcca atgttgttcc agtaccgata acacgtgatg ttggcagacc
      961 agattcgttc caagcgatat atgtcaaaat atctactggg tttgaagcaa caacgataat
     1021 accgtcgaat cctgatccca tgatttcttt tacgatttgg cgcatgattg acgcatttgt
     1081 tttaactaaa tctaaacgag attgaccagg tttttggttg ataccagctg tgataacaac
     1141 gatatcagca tctttacatt cttcatagcc acctgaccat acagccacat tttcatcgcc
     1201 ccaagccata ccatctaaaa ggtccaatgc ttccccttct gctttttctt gattcacatc
     1261 gatcaatact aattcattag agattccttg gttgatcatt gcataagcga tacttgttcc
     1321 aacaaaacct gtaccgacga ttactacttt acgacttgtt tttttcatga ataaacagct
     1381 ccttttcata actatttgtt actttcatga aaagtatagt cttgtttatt cattttatca
     1441 acatgttaat tataatagtt gttattataa tttttgatgg aaacgaattc ctgaatacgc
     1501 ataacagata aatgacaaat gtttacacct atatcccctg ataatacaac caaaagttta
     1561 gaactgaaaa taaatccaaa aaaattcgct gcaacgatat aataagacag caatcgcacc
     1621 taaaacaatt ttccttcata tgctttagtg aaagattttc ttcttaatgt ttatttctcc
     1681 attcattggg agtacatgtt gaatacttct tgaataattt gaataaagaa gtcgtttgtt
     1741 tatatccgat acgattagcg atttcttcca acgaaaaact cgtttcttga atcagttcca
     1801 acgcaaagtc gaattttttc tttagaacca gttcttgaaa ggaagcatca aaatacattt
     1861 tcaatttatt actaagatag accggattgt agccaaaatg agctgctgtt tcttttaacg
     1921 tacaagaagt aaaatgctta tcgatataat ctaatactct aagcatctca ttttctgcgc
     1981 atccatctgc tgatgaaaag ctttgttgtt ctaataaatg agtcaattcg atcagtaata
     2041 gtgatgtata gagatttaac gaacggatct tatgactgta tgtatcaaaa taaatcaaca
     2101 gcatattttc aatcaaatgc gtcaaacgac ttgtttttcc gccgcgaaaa tgaatgaatt
     2161 gtgtatgttc ccctttttct tttgatgcat ttatcaaaaa agaagaaagt agattatttg
     2221 tgcttgccaa atgattcaat atctctgtag ataaggtttc ttcttttaat aagatgttga
     2281 ttaaaatatc ctcttgccct gtgtattcga ttctttgagt agcttcttta tcaatgatca
     2341 ccacgtcact ttcttccatc tctatccttt ctccattaat aatttgtaaa catgtaccgc
     2401 tatacatata attgatttca acaaaatcat gtgtatgctc gggaacttcc gcatatcgac
     2461 ggtgtttact gatatgaata tttccctgtt gaaatagatc ccctcggtgg atttttggcg
     2521 gtgtctgaag atggtaagct aagtttaaat cattgatatt cattccttgt tcacgctgca
     2581 gagtttcgat tgattcttcg ttacgcaaaa aaatgtctaa ttcagctagt tccaattgac
     2641 tccctccttt ttgtaagcgg tttctttcgc ttattagttc acgctgtact gctattatat
     2701 gcaaagctat ttttggtttc ataaagactt tctatggttg ggacatgaac ttcctgtgat
     2761 tccgctattg agagaataac cacttcatac tatatttttg ataagcgctt acaaaaatga
     2821 aaggaacatg cttatgaata aaaagatgat gaaattttct ctgctgtcag tctctcttat
     2881 tttgacttct gctggtgcag tcacgtctgc aattcccagt atgtcagcta cttatgccac
     2941 gcgttcttta tctgctgttg aattattaac gactgctccc tcttttatgg tcactatttt
     3001 tgttttgatt agtagtttta ttgctaggaa aatcggacaa aaacaaactg tgatcatcgg
     3061 attagtgatt gcatcgattt gtggtatcct ccccctcttc tcacaaagtt acagtattgt
     3121 cttagcttca agagctggac taggtattgg ctttggattg atcaattctt tggcagtcag
     3181 catgattgct gccttttatc acggagatga acgagcctct atgattggtt ttcaaagtgc
     3241 atttcaagga tttggtgcag ctttaatgac atttgttgca ggacagctag tacagttcgg
     3301 ttggcaatat acctttttag tctacgccat cagcttacct attcttgtct tgtttgtttt
     3361 gtttgttcct acacctgaaa caactgaact tcaatcgcat ggcacagccc caacaagaca
     3421 agtaatcaaa acgaatccta aaatgattag ttatggtatt tttctcttgc tggtcatcgt
     3481 ggtatataat gctgtatcga ttaaattagc cacagtcctg acgacttatt ctctaggaaa
     3541 cgaaactaat gcagcgaata tcttaacaat catgcaattg gcaagtatgg ctgccggagt
     3601 ttgttttggc tggcttcaga caaaagcaca tcgctatatg cttacacttt ctttactact
     3661 gatgggaatc ggctttgtat tgattgccac tgtgataaac ctgtatgtga ttgggtttgg
     3721 tgcgatccta acaggaattt ctttttcgtt attcattcct tacttattca accaagtaag
     3781 tatccaagcg cccaagtacg cggagagttt caatacttca atcttattgg ttggcgctaa
     3841 tttaggtact ttcattgcac catatggttt gctctttctc tcttttgctt ctcttgggag
     3901 taaaacactt gctgttttcg tcaatggcag tatcttgctg ttgattagcg cgcttattag
     3961 ccttctagta gtacacagga cgaatagaaa gacagtgata agagtagatg aagaaataaa
     4021 agaacaattg acaacaaaat aaatttgatg gaggtcgtgt aaaatgacaa atgaaaaaca
     4081 taaagataga tggctttggt atcctggaga ctttgagatc cgccatggac tgctacaaaa
     4141 tttccaacgg gaagaacgag gttttgactg gccagcgtac tggtacatgg atgattgcca
     4201 tcggaacgtc aagtttaaga gatattactt tcttgatcaa ccttccatgt ttaaagtgac
     4261 gatccaagga gttgggtatg tagaaattaa tggacaaaaa catccatgtg gaaaatggct
     4321 gacttgtcca gctggtaaag caaaaattcg tatctttgta ggacatacat cagggctacc
     4381 agcgatgttt atcgagggag acgaagtaaa atcagatatt ggctggacag caagtaattt
     4441 tattgaagaa tatccagctg gctggagtcc gctttatgtc gatatcgcaa aagatccaaa
     4501 tcaaatctat tatcaaaaag aatgcgtcca tccagtcact gaaacggaga ttggcggagg
     4561 aatcttattc gattttgggc gagcagtgaa tggctgtgta gaactaatat ctctggatga
     4621 ccatatcgaa tctatcactc tatgttatgg agagtccgat gtagaagcta tggatatcga
     4681 ttattgctac tataagcaaa taggaatgac aagaaaaatg aaagctcgta aacgcgcttt
     4741 tcgctatttg tttataccag aggtttctac tggaatggtt caacttcaag tttttcatga
     4801 atttttatct ctttcgaaac aagctgcttt ttcaagcgat tcttctttac tgaatcagat
     4861 ctggtcagta tcagaagaag ccttcaagtt atgtagtgat ttgtttttta tcgacggtat
     4921 caaacgagat cgctggattt ggtctggaga tgcgtatcaa tcattcatga tcaatcaata
     4981 tctttttttt gacgaggaaa tcaataaacg aacgatatta gcattaagag gacaaaatga
     5041 aatcaagcag catatgaata ctatcgttga ctattcgatc ttgtggttga ttggtatcga
     5101 aaaccactat atgatgacga aagactatga atttttacgc atcgtctacc ctaagatgca
     5161 atcattaatg agatacttga tggagcaaac gaatgaatta gggtttattt atgggagagc
     5221 aaaagattgg atatttattg attggtctga aatggataaa gaaggaacgc ttgctgcaga
     5281 acaagtctta ttactgaaag cctatcattc catgatcact tgcggagata tcttagctga
     5341 aacatatgga gagaacgaaa attatcatac tcatctctat caagaaaaat atcaactatt
     5401 gaaacaaaac ctatttgact acttttggga tgaagaactt ggcgctttta tcgattgcta
     5461 tgaatctggg agaagaaatg tgacgcgtca tgcgaatatt ttcgctgttt tattcgattt
     5521 tgctaacaaa gaccaacagc aatcgatctt aaagaatgtc ttattgaatg acactatcac
     5581 acagattact actccatatt tcaaattcta tgaacaagat gctttatgca aactcggaca
     5641 gacgaatatc gtctaccaaa cgattcttga ttattgggga gggatgttat ctcatgatgc
     5701 ggtaactttc tgggaagaat acgaccctac tcaaacagga aatgaaaagt acagtatgta
     5761 tggagatcct tatggaaaaa gcttatgcca tgcatgggga gcaagcccaa tctatctttt
     5821 aggacgctat tttatcggtt tgtatccaac aaagccaggt tatgaagaat ttatgataca
     5881 acctaatcta gattcatttg aaacactgaa ttgtacgcta cctatcaaag atggaaagat
     5941 ttcgcttgaa aaaacaaaag ataagatcgt gattacttct gatagaacag gcggaacagt
     6001 gaaaatgaat ggtaaaagtt atcctcttct agcaaaacag ccacttgtct taacaagcta
     6061 ttcaaaaata aatgcataaa acaaaaaact gcttgcgaca tatcagaaga ctttaaatct
     6121 tcttctgata ttgtccaagc aatttttatt ttgaagtttg cttaattctg ttctttagaa
     6181 acaatgtttg ttctttttcc ggcaaaaaga aagctagata atacgacaag taaaaagaaa
     6241 gcgatgattc caactgtcaa ggctatttga ccgtattctc ctagtttccc aagtacaagt
     6301 cctggtacta gataatcagt atcggcaaat gtagaagaag cgattttaag atctcctaat
     6361 actggaagga taaataatgg cagccaacta atcaataagc tattgaaagc agaacctaat
     6421 accgcccctc ttctaccgcc agtcgagttg ccgaagacac cagaagcagc tccgcagaag
     6481 aaatgtccta ctaccccagg aatgataact gtagtttgca agcctatcat agcaaacatg
     6541 gatagtgttc ctacaaagaa gctaacaaaa aagccaatta atacagcatt aggtgcatat
     6601 ggaaaaacga taggaacatc taatgctggt ttagaatcgg gtacgagttt tttagcaatc
     6661 ccttggaatg ctggaacgat ttctgccaac accattcgaa ccccttgaag gacgataaca
     6721 aacccagctg tgaatgttcc agcttggatc agtgcaaaga ttacagcgtt gtctccattg
     6781 cttagttctc tttcaacaaa tccaggacca gctaacaaag caagtgcgac atatacgatc
     6841 gacatcaaga gcatgatcga tacagtagaa tctcgcaaaa agctgaaact tttaggaatc
     6901 tttatgtcct ctgtagaacg gtctttgttt ccaaaaaact ttccgatcca tccactagaa
     6961 gcataaccga tatttccggt atgccctaac gcaacttgat tgtttccagt gatgttcttc
     7021 ataaacggtt gacaaagagc aggcgtaata gttaatagtg ttccttcgaa gaccccgcct
     7081 aataagatca tccacaaatt tgcagtaaac cccgcgctta ctaaaatgac tgctgttaga
     7141 caagaaacat aaagcattgc ttgacctgta agaaaaatga atttatatct tgtgaatcta
     7201 gcaagtaaaa tattcacgat catccctact agcatgatca aagcagtagg tgtaccgtat
     7261 tgttgcaaag caatcgccac tactgcttca ttgctaggaa ctactccttg agtatgcaga
     7321 gctgtttgga acatcgaagc aaaaggggtt agtgaattac tgattaaacc agcaccgccg
     7381 atcagtacta ggaatccgac aaatgttttg atagttcctt ggatcacttc tgaaaatttc
     7441 ttcttctgaa gaacgagacc aagcagtgca atcaaagaaa cgaggatgga gggctgactg
     7501 acgattgaca caaacaaatt caacaattta tccattgata gttcctccct tgaatcattt
     7561 ttattctttt gacaagatct tcgttacttt ttcttttact tcttcgctgt caatgatcga
     7621 ttgcaaaggt acgagctttt catcaggtat agaagttagc gtatctttca aagaatgctc
     7681 aatgaagaaa taatcagcca tatcgctaga tacacttcca acatccgtat gacttacttc
     7741 aaactcgtcc acatctgctc caagatctgc aagtgcttgt ttgatgttca tctctaccat
     7801 aaaacttgtt cctaatccac tttgacatac tgcaaaaatt ttcatatcat tcgtctcctt
     7861 ttcttatgat cgctaatatt tcttcttttg ttgatgcgtg taataaatct tgaagatttt
     7921 gtttattaga aagtagcttt gttaaatttg ccaaagcccg aagatgtgcc tcattatcga
     7981 tagcagctaa acagatgaac aaactaatgg gatgggctgg attatcaagc agatccacag
     8041 gttgttgaag tttcagcaaa gacatcccta cttcgtttac gccatcttct ggacgagcat
     8101 gaggtaatgc gatgtgatcg ccaatatgga taaacgcacc atactgctcc acgcgctcga
     8161 tcatagcttt gatataactt tcttctattt tattttctga taatagcggc tgtgcggcaa
     8221 atgtgattgc ttcttgccag ttttttggtt gatccgataa ttggatcatt tcttctgtta
     8281 ataattcaga tagcatagga cgttgatcct ccttcttttc taaaagtttg ttcattttac
     8341 gatttaatac ttgatagagc ttttcttcgt ctacgccttc ttttaattcg atataaggca
     8401 gtagcgccga caacaattct tgggcagaag gcattgaaaa tcctggtatc agccaatctc
     8461 tttgtacttg attgatcagc tgattttttt ccaagtcagt catgattgga tgcagaacat
     8521 agagagactt tttcttatca gtaagtgtga gaggtacagt agaaaaaata atatcgtaag
     8581 aagtttcgtc aacctcgtgt aagcggtcag ctgaattagt ctctgtaaaa aagatcatcg
     8641 ggaataattg ctgaagttca ctttttaaga tcaatgaaga gctaatgcca cttggacaga
     8701 cgatcacggc tcttattttt ctatttcttt cttctgcgtc cgcttttctg atctctccgc
     8761 caaatagtat agtgaaaaaa ccaatctctt cttcagggat gctcttccct attcgtcttt
     8821 ctaacggaag taatgctttt cttgtcattt cgtagatgga agcatattga tgcttaattt
     8881 gatcgatcat cacattaggt aaataaaaag aataggaaat acgaaaatag gcagggacca
     8941 agtgataaaa gacattcatc aataattcac gaaacgaagc aaattctacg gccatcagga
     9001 attccatgcg atggatgact tctgaagaac attccagcaa aaaatcaagt gcaggctcct
     9061 ggacatctcc ttcaacgatc gatgctaagc aaagagagaa aaatataatc tccgaatttt
     9121 gatgttcgga atagccaagg ccagtcaata cttgtcgact aatgtcagtg actgcaagag
     9181 aagccaacag ttcagcatct tttaccagtt ccatggatgt ttttccaatt cgaatggttg
     9241 tcaaactcaa ataataagct aattcatcca ttcgatttgg tgcgaatcga agatctgata
     9301 gtgaaatcgt tttatctagc aaatctctaa ctttggcgta ataatcaatg gaaagggttg
     9361 cggttaaata cgtgagccca aatcttccag caagtgactg catcaaacga gcagtgcgat
     9421 tttttgcttc tcttcttaat attgcttctg accctagcag cctaaaacca gttttcctag
     9481 tatacgacag ctgacaatct tccacttcta attcatttct tatctttttg atatctgaca
     9541 ggactgtccc tttagaaacc tgaagaaaat cttgaaaatg aaaaacagaa acagacgttg
     9601 cagtcgaata aaccatcaaa taaagaagat ctcttcgctc ttgttcggta tacacagctt
     9661 caaatcgagt aatatccaaa aatttttctg cccagtcgat tgctcggaat ttctttatac
     9721 ggatacgggt atcatccaca tattcggcaa aaggagttaa ttgtttgatc gcctcgcttg
     9781 tatttacttc gatgatctct gctgcttttt ctagactgat ttctcttggt gttttcagga
     9841 gataactaat tttgtagttc acagcgctgt tcacctccta cttatattct agtatggtag
     9901 cgcttttaaa tcgaggggtg tttggtacaa cttacagttg gatttttttt acgactgtac
     9961 cgaacgattt ctatcgaatg gatacagaaa aaaagcaggt gaccaaatgt ctcctgcttt
    10021 tatgaattga tttcgtccga tcaatgatga tcatgtttat tttttgaggt cagttcttca
    10081 aatttttctt tgacatcttc aaccgctttt tcgatatctt tttcaaagtg attgggctca
    10141 ttaggctttg tttcctcttc tacagaattt gaaccagata aagctgtttc tttattttct
    10201 gtttcaccag agtttgtgcc agctaatgat tcttctttgt cttctgtttc accagaatta
    10261 gttcctgcta aagccacttc ttcttctgtt ttaggggcca ttgaacctct taatcgtttg
    10321 tgtttttcac cgtcataatt tgtcatgttg gttcctccta gtagttctat actttttcat
    10381 cgtacaataa gacagtttat gcgtcaaaaa acaaacccga ttcttacaaa tatgtcatat
    10441 ttttccttct gattcttgaa tgcttttgat tccttcttcc aaaattttca tttgtaagat
    10501 tgtttcttca tctttgatga ctttttctgc gccattaact aatgtctcgt aaatcccttc
    10561 gtagacacgg ctgtaatcac caacctcgga aattactttt tcttcatgat aactcccttg
    10621 ttcatctata taggtcaata caccataatg ttcgggcaaa tcgataccaa agtcttcatg
    10681 atcaggcatg taaaacattt tcagatgttc ttcttgtcga tcttttgttt gtttcacgaa
    10741 catccctttt ttccgtaaac gacgaaactt ggacgttctt tgatgcggaa ataactggat
    10801 tttatcgaaa ttttcatatt tccatagaaa aaatccagat cgaagtaatc attcattcgt
    10861 cctttcccta aaagttgacg tacatcatag ctgatatcat ctggttcgcc gaaataagaa
    10921 agaacttgat ctactgtatg acaagcatgt ccatataaat agctgttcgc agtagaaaat
    10981 tcatgcgtat tttctggtac ttccggtctg aaataatcat aatgcatttc tacttctaaa
    11041 atatccccta attttccgct ttcgattact ttttggactg tcaagaagtc agaatcgaac
    11101 cgtctgtttt ggtaacattg gataaataga cctttttctt ttgctaatgc aaataactct
    11161 tctgcttcct ttgacgtttc tgcaaaaggt ttctctacta gtacattctt accatgttcg
    11221 agtgccattt ttccatattc ataatgaaat tgactaggtg tagtgatgac aactaattgg
    11281 atctcaggat catcccaaag atcagacagc tgatcagtat attcgatgtc ttcgaatgtt
    11341 ttccacactg gttcagatgg ggaaaaaatt tttttgatat tgattttgtt cgtaagtttt
    11401 cttgaaaaag gcaaatgata gcgatttgta ctctttccat tgccaagata agcgattgta
    11461 agcatgagtg gtgcctcctt ttattgatgt tttcagtata tcctagaaaa gcgcagaaag
    11521 tatttataat agctattctg gtaaaaaagt tcagaaaaac acttgttatc attcggtttg
    11581 gtgaaattaa tgttcaaagt aaggataaaa atcaaaaaga tagtagcgga accaagtgtt
    11641 tatcagtaag atagaatttg gaggtcagat catgcttatt gaagaaaaac taacaaaaca
    11701 agagttgttt accacgacag agaaacggat tgtcgattac atccgtagga atatcgaagc
    11761 tgcagtctac atgacgattg aagaattagc aaaagctacg tatacttccc attcagctat
    11821 cattcgttta tgcaaaaaaa tgggattcag cggatttaaa gaattccgtt tcgagctggg
    11881 gcgggaagtt catcaattga ttgctcagtt caatcaaacg attgatccta attttccttt
    11941 ttcaagtgaa gatagcactc agcaaattgc aaagaaaatg gcggatttat cgatccagtc
    12001 aataaaaaaa gcacaattgc agattgaaca tgaagattta gatcaaatcg gtaaagtatt
    12061 gaacaaggca cagcgaatct ttctttttgc aaaaggtgat tctcaaatca cggcaaggaa
    12121 attccagaat aaaatggtca agttgaataa gtttttgatc atggctgaag aatacagtga
    12181 ttcttcttgg aatgcagcca atttgactag cgaggattgc gccattttta ttagctacag
    12241 cggtcgcatc catcattatg aacgaataat gacctatttg aagcatgtag aggcccctac
    12301 cttgctgatt accggaaatc agcattcaga aatggccaaa caggctgata tgtgtcttgt
    12361 tatatcgcag gaggaatatg attttgctaa agtagcgact ttctcctctc aaattgcctt
    12421 cgattacgta ttaaatacgt tattctcagt gatatattct caaaattttg aagagaatat
    12481 ccgcaacttg aaaaacaaac aagaattgat gcaaaaaggc cttcttaaag aatatcggaa
    12541 aacttaagaa aagatcctca tttagtaata agaaagatag tgtttgatag gctctctttg
    12601 atatcatcat aaaaacaggc tgaagttttc tttttcaaac ctcagcctgt ttgtttacgg
    12661 aacgattcga atagatatat tatagaaatg aggaatagct aacttctgtt tcttattttg
    12721 aacgattgac gaaaaagata tataagaagg aggaaaatca gtatacatgc agctaacaat
    12781 ccatacagga aaaatcgttt gttctcggat actttctgta cttctttttg aacttctttt
    12841 gcatgataag gaacccggtg ccctctgaca agtaagcgat gactgttgac catataaggt
    12901 gtacaagtta ggagtgtcac gaaatcctgc cctttttcga ttctcagatc ttttgtatca
    12961 gtcggttcta ccgtcttgat ctgatcgact tgataagcga gtgtccgtcc attgatttcg
    13021 atatagaaag aatcacctaa agtcagtttc ggtaagtctg tgaacaactt tgcttgaggc
    13081 agtcctcgat ggctggaaat aacggcatgt gtattttctc cccccgtagg ataagacgtt
    13141 ccttccaata atgaggcacc tttttctagc agcacagtat tcgtttcatc aaaaattggc
    13201 aaatggacat tgatctcagg gatcgtcaaa ataccgatcg tatgtttctc aaaataggtt
    13261 ctatcctttt tcgcaggttc ttttttctta gtaaagggat ctgcacctgg atttccacct
    13321 tcttttgcta attgctggtt tttctttgtc atgttttctt ggattttagc catgacttct
    13381 tcattttctt tgaccgcttg ttgctgataa tgagagatga tctgctgatc cagataatta
    13441 ttcagcgcgt cgctaacaaa tggataagct agtatcccta ttcctgagag aagcaagaaa
    13501 agcatcagca gatctagaag tttccttttc tttggtttca tttgtgttct ccttgtgatg
    13561 actgtttgat aataaaagaa aaaggagcat ttgcgtaatc cgctgctcct ttttccaatt
    13621 cttgcaaaaa gactaaatct gactgtgctt gcgtctagca aagtacagtc cagcaatcag
    13681 tagaaggact actcctgcac cgatatacac atagattccc ttaccgcctg ttgaaggaag
    13741 tgtacctttg tgtttatttg gtattttttc aggagaaatc aactgtcctg ctgttacata
    13801 agattgttca tcgatagtaa atgctacacg gtttgttaat ggaacatatt tttctggcgc
    13861 tttcgtttct tccagatagt acgtgccata ttttagacct gtcacatcga ttaaaccgtt
    13921 acttgtggtt gtaaaaaccg ttgctgattc tttcgccgat acccagctga cggcttttgt
    13981 ggatgggtcg atcgataaat atttcgctgt gtcactatct tgatcacgaa cgacgaattc
    14041 tgctccagca agtgtttggt ctgatgtaac gtcaccatct actttaacaa atcgtttgcc
    14101 cccagtaacg acatcgactg acggtggtgt ttgatcattt gtatgcccgt tatcgacatt
    14161 tgcttggtta gaaaatcctt tggttggatc tgctttttcg ttcaaatgca tatagtaaac
    14221 gaatttcaat gtaccgccag gagttaatga aggaatataa ttcggatcaa ctgaaaccgt
    14281 gaatccgtct gtttgctcag tgacagaata atttactggg tcgatttctt tatttccatc
    14341 atataaggca taagcatacg ttcctgaaga atcattatca aatgttaaag cagcgtcatg
    14401 agtatcgata agtttgaatg ttgtgtactt gttttgcgtt ccttctttat cagcgatacc
    14461 taatgggata ttgacagaaa tctcatattg aattttttca ccgattgcga catcttttcc
    14521 attcaattga ggtgttgttt tatcaacttt agacgtatca tttttgatgg tcttttctac
    14581 tggtgtttgg ctatttgcca ggatctcaaa aggcgtgatt gtttgctttt caatcatttc
    14641 cgcattatca ggagctttta cttcttctaa attatatttt ccaacttcta aatgattaac
    14701 gatcaactgg cctttttcaa tagatgcctc ggcaaagtca ttgttgccga tgtcataaga
    14761 atgaccagta atgaaatgtt tggctttggt ttgatcagtt gtccaagtgt acaatccatc
    14821 tgtgacactt tggatgtatt ttttgacgct tggtgttcct tcttctttag aaataataaa
    14881 ttctgctcca tttagtgctt cgttttcggc agtaccgatt tttgtaactt tcaacgttcc
    14941 atcattaccg actgtatttt tagggtagag atggatagta tctagttctt ctgtcccgta
    15001 tttataagag ccatctgctt gtttgatcat ctcatataca gggaaagcta aaaccatgtt
    15061 tgcggcagct gacactccgt cttttggttc ttctttgatc gtgtagactg catctttccc
    15121 attttgtttt ttaggtaaag ataaagtgac atttccatca gcatctgtcg ttcctgaagc
    15181 aactggtgta ccaggagtca aagactggac tgcttgtttt gctgcatcca cggacgctcc
    15241 tttatctcgt tgcgcataaa attcttgagt gacgttataa actgaaaatg aaatatcggc
    15301 taatccttgg tattgatcga attcgctcat ttctttcccg ctgttttgga ttaaaggatc
    15361 gggtaaatca gtcattttct ttttatgaag gatgacttga gctgctgtct cctctgcaaa
    15421 cacttttttt gcgccgaagc tgtttgtgag taatggtaaa ataaggaaaa ggactcctaa
    15481 cataacgttt atttttttat gatttttcat tttttcactc ctgttctttt tttgataaac
    15541 aaccagattg caaagcttgc gaataccata ccaacaacgc ctaaaagtgt atttttcgtt
    15601 tcgtttgttt ttggaagaaa tggacggcta ttttcttttt tattgactac tttcaatata
    15661 ctgtccgcct ctttatcctc agaaagaaaa gttgaaatgg taaacggaat ttctgtttca
    15721 cttaaaatat aaccagaagg tgcttcgatc tcttttaatc gataacttcc tgttttcaac
    15781 ccggaaattg aaaaagcgcc atttttatcg gaaatcaggt gaagcgcatc tgattcgttc
    15841 tttgtccact tgtatccgtt ggctgtttgc tggaggtaat ttcctgcttt gtttttaata
    15901 aggaatactg cgccagctaa tcgtttatct ggtttatttg tctcgatttt gacaaattgc
    15961 ttactccctg tatatacaga gactttcttt gtactgacac caaaaccaaa atccagtgtt
    16021 ccgttgttgt tgatttcctt gttcgcctgt gctgtactgc ttagctgcat ctgataagaa
    16081 atggtcagtt tttttccagc atatttttcc aacgaaggga tcgaaaataa aacatcaaat
    16141 ccgtgaggtt tcttgtgtat gacaaagcct tctgtcagct tttctccatt cgatgaaatc
    16201 gttaaactat taggtaaaaa cgttaatgct tcatccgcac tatctgacaa ttcgaacttt
    16261 tgatagtcaa ggatatttac aggaattgtc gtatgcaagg aataagtgat tttttcacca
    16321 atcgtaaaat catttctagg ctcttctaat accttctcaa aaggtggatc agggtagtcg
    16381 ttttcttcat ttttaggata caaatggata gttgaaagtt tttgattgtt ttgtccatgt
    16441 acaggaagaa caactaccat attctctgcc ttttctttga cgacttctgg tactttggat
    16501 tcaatgaaaa gatacgcttt atctcttttt tcttgatctt tagcggccag agaaaaagaa
    16561 gcgataccat cttcgttgtt aagagttgta gttgttgctt ctgcggtaaa cataccggaa
    16621 gacgcaccgt tttttgcgat ctctgcttgt gcttcttcta ccgttttgcc cttttcccgt
    16681 agatggtaaa aagaatctgt gacatcataa acttggaatg tgacaccatt taatcctcga
    16741 tacgtttgta ataaagcttt ttcttgtccg tcatttggat gatttttcgg cagttgcccg
    16801 tttgggaaaa gcaatttgtg taaagtaatc tgaaccattt ctgtttctgt ttctgttgct
    16861 acctgagtaa aagctggttt aaatagtaac aagaagagac acatactaag ccaaccaagt
    16921 tttttcatat ttagctgccc cctctttctt ttttcaaaaa aagataccaa atagaaaacg
    16981 cacaaccaat catccctact agatagattc ctaaacggcc aattcctccc gtttcaggta
    17041 atggtacttt tgcctgattc gtgatgtcta aagaaatctg gttgtttttg gctcctggtg
    17101 acagaacaga ttcatgatct tgtccatcca cttgaattga cccatcttcg tgtataacta
    17161 tagtaactgg ctcttttaga ccttggtatc cttctggtgt aaaagtttcg gtcagcgtat
    17221 aagttccagg ttttaaattt tcgaatagaa aggtatctgt ttcttgtcca tcttttggcg
    17281 attctaagtc catttctggt ccctgcagtc tgaattttgc tccttttaat gtctgtccgt
    17341 tgtcttcttt tttgtgtacc gttagatcaa acgcttttag tcgattttga tgagtgagcg
    17401 tccatacatt atttgtcatc tcggtatttt ttcccgtgta aatgattttt ccatatttat
    17461 cgatttggaa ttcataattt cccggcgaag tatcatatcc ggcaggacca gctgtttcaa
    17521 cgactcgata tttaccaggt gtgagtgaat caaaactggc aagacctttt tcattcgttg
    17581 tttggctgtc aattggctga taagtaccat ttgcttcttt tttctgtagg gaaaatgctg
    17641 ctcctttaag atttatctct ttgtccgtcc cttgcatcgc atacttacgt actgctacag
    17701 gaacttcaac aaaaggattt tcaatagtca actggatcgt atcatcggaa gtagtgactg
    17761 tttttccatc aatggttacc gtaccatcag aagcgatctt gatttcccaa gtagtctttt
    17821 tgcttaatcc atgcccttct ggagcttttg tttctgtcag cgtataggtc atttcttttt
    17881 gcaatttgac attttctgga agagaatagg ttccgtcgcc atgatctgtt agtaaagtat
    17941 caatagaatc tcctgttaat ttgaaaacag cgccaataag atccttttca cctgtagagg
    18001 aatttttcgt tattttcaag tttaacggtg tgaattgttt agtatttttc catgtcattg
    18061 catctatttg ttgagaatcg tatcctggta caggtaattc tttgattgtt tggtaactga
    18121 atgcttgacc ttgattattg tattgaggta gtgataatat ctcttgataa ctttctccgc
    18181 tatttgcaga taatttgtca atgtctgcac gttcccacgt atttgttgta tctttagctg
    18241 gtttaatgat tcgaatatat ccgtttttcc aagctgcagc atttgtcgta tgttcccgtt
    18301 gaatctcaaa agtaacttga tctggacgat cagctagatt gttgtcaaat tcttcccata
    18361 actttttgat gtgaagactg actccgggag ctttagcaga tggtataccg aattcagcta
    18421 attcattggt atcaatgttt ggctggaaag ttgtcctgcc gttcatttga taccagaaat
    18481 ttggatggaa gtcctcattt tctgtttgga ttctcacttg gtaatggatt tggatttctt
    18541 ggtcttttcc taaatagatc tgattgctct tgatggtatt tccttctatg gaaatagatg
    18601 gagtgaccgt tgtaggactt gtccccacac tcttgactga aagtgtacca ggctgataaa
    18661 tgaatggttc tgcgattgga tcatttattt gtccattgct tacagttgct gagatctgta
    18721 cagctttttt ggctaggtat tcagagatat ctgttgcatg atcagctgat tcatagtaaa
    18781 gatcgccttt ttcatctgat gaaaccattt gtcgcatacg agactctact tctgcttttg
    18841 agagaccagc tgccggatcg ctttgaagtt ggatgccaag accatgtatt tcgattcctc
    18901 gttctttgag tgccatcgct tctccgatgg ttgcgataaa cgtactgtcg attcttctgg
    18961 atagattgtt ttggtcaggt gcatcataga ttcttgagat tagagaagta tttcccggcc
    19021 gatcttgcgt attagaaaac tgagttccgt aataattgct gcttgattgt gcgtgtaccc
    19081 gctgtacttt ataggaaaat gttggtacac catccgtcag caaaacgatc acttttttat
    19141 gtccatttgg aacggatagc atgcttcctg catctcttag tgctttttgt gtaaaagtac
    19201 caccatttgt ccgtgaaggt gtaatggatt ttacttgatt tttcactgaa tcaaatgaac
    19261 ccatctgtac tgcaccgtta ctgtagctat atccttcgct tgagtagccg acatatccca
    19321 tattgatttt gtctgtgata ccgctatctg ctaaagtatc gacaaaacga tcgacaccaa
    19381 tctttacttc accgatccga ttattgtcgt tcatacttcc tgaccagtct acgaccaata
    19441 cgagatcaag aggcgtgata tctttttgta catttccacg agcattcaaa taaacatcaa
    19501 acaatccggg tgtacttgtt tcctttgcat actttcgtat cgcatagtct gcttgattac
    19561 cggttcctcc atattcgata tacgaatggg tccgatcatt aggatctcca ttccaagaat
    19621 ttataccatc ccattgattt gttccgtttt tattgccttg atgatcaaga acatttgtat
    19681 ttcctgtagg ttgccaactg gctttaggat aagtgccctg atcatctgtt gtatattctg
    19741 taggaatcgt ttgattagat ggcacactca cagctggcaa ggataatgta ggcaatgtaa
    19801 atgggctgac agaattttcg ttttcttttg atgattcttc ttctatttcg tctggctttg
    19861 gtactgacac atctgtatct ttcttatttt ctgtctcatc aataatgtca tattctttat
    19921 tatcgacgat cagcttgttt gcctggctgt ggtaaagtgc tggatcgatt ggaaaactca
    19981 gatcgatagt ttgtttagtg tttgatgaga tttccaacgt aattttcccg ctatttacct
    20041 gataagcata gccatctcct tcttttcttt ccacatcact taagtaattt tcgactagac
    20101 caatttcttt ttctatcttt tcagcactgg tgttattcgc atttagcttg atgttcagtg
    20161 ctttttttcc agactgatcc gaaatgactt cagctgaaat tgtttctaaa actgttggat
    20221 catctgtagt gtctgctatt gcagataatg gcacaaagtt tgataaactc aatattatca
    20281 gcatgaaaat aactttcagt ttcttccctg tggtcgtcat ttttcccctc ctttttcttt
    20341 ttttctgttt tgacgtgaat agtgaaaatt attaaaataa acaggcgtta cataatttta
    20401 tttattttaa tatgataatt attatacgaa gggcgctaaa gggaatcaca ccaattttca
    20461 taccaattgg tttcatttat gaagttgatt ccttcgatta atgagcgttt cattgaaaga
    20521 aaagggttga gggagaaaaa catatgatca ggttgttgga attaaactat agaaagatgg
    20581 aaaaaatcat tcgaattctg tccatttctg acaagtggat gactaaaaaa gaacttgcca
    20641 aacgaattgg tagttcagaa tctacatttg taaggtatat cgaagagatc aaaatccgct
    20701 ggggaacgga tgtaacgatt caatcttcac aaaaattggg gtatcgtctt gaacatttga
    20761 atgtaccagc ttatttaaat gttctgatgg aaatgtcgca aagttctaca aacagtcgtt
    20821 tattaaatga gattattgtc aatccgggta aaacgattga gtattattgt gaaaaaattt
    20881 ccatcagccg gtcttctttt acacgtaagt tgaaacaatg caatcaaatt ttagaaagct
    20941 atttattaaa agtgatcgtt gaccaaggtt ttcagttaac tagccttaaa agtgagctcc
    21001 agttgcgtat ctttgttacc tttttctttt taagttacta cggaagacgc acgttgccat
    21061 ttccaatcga aaaaaagaag atcaaagaaa ttatggtcaa gaataattgt cagatgaatt
    21121 tgtttcagga agaaagccca tatgagcaat ctttctttat tatgtatttt gttgtgcatc
    21181 tcatgagaga atcccaagga actccatttg aatggaaagc tggagactct tatgtacagt
    21241 tgataaatat cgataaaaaa gacttcttaa tcttgaaaaa tctcttagga aaattgaagt
    21301 acagcagcta tataaaagta gtgaactatt ttgtcaatac gattttttca gaattttcgg
    21361 agaaagaaaa aaatgaaatc agtgagcaga tacgaaaaaa tatgtcctac tgttcgtttt
    21421 catccgctga ttgcaaagaa aaaaacgaag aatatgttgt tcaattatta acgcaaattt
    21481 attttttctc ccgtattttc ccatacgata cgactcattt gacgatccgt gcaacttact
    21541 ttgcacagca gatgcaacac cgccagatac gtctatatgc tggatttcgt gcagaacttc
    21601 aattttattc cactctactg aatcagaatc tgatttatta tttatctagc tatatctttt
    21661 ggatgcttat cggtaatcct gaaattcacc attatacttc tgataaaaaa atactgatta
    21721 tcagcgactt aagtcttcga catagtcaat atattgaaga atatatcagt gatattcttg
    21781 ctgtgcataa aattcactct gagacaactg ctattactga agatcagctg tcgaaataca
    21841 atcttctcga gtatgatctg atcgttacga atcaacccat tttgaatgcc catgttcctc
    21901 atatattgat tgatgatagt gtgtcattcg caaatgaaga agaactcatc cgcttattcg
    21961 agttatgaat aatcaaaaaa cagctatgta taaaccaccc tgataaaaat ggctcttcgt
    22021 caaattgtgt tggcgaagac aaatttgaat aaaacttagt ccgatatgcg gcgcgttctg
    22081 tgcgccgcat atttttttag ttttcaaaga tcagaccttg ttgtaaatag atccgtgccc
    22141 gaaagttata aaagttgcga taaccaaacg caattcgttt aatcagcttg atcttgttat
    22201 tcagcccctc agtgacgccg tttgagtggg gcaccttaaa ggcatttgaa atacccgatt
    22261 ggtagcgatg gaagatcgtg aatttctttt taaacgactg aggtaacgta gatgaaattg
    22321 ttttaagggt ttcttgaaag ccttccgtat ctcgtttaac gaaataatag cgtaataatt
    22381 gacaaaaatg atacgcagtt cgcaattctt cgttgtagct taacagctca tcaacgattt
    22441 ctgtttgact aattggtcgt ttaaacagtc gatgataatg ccgtttgagt ggctccaatg
    22501 tcgtggaatc tttgagtaag agtttccaat atcgcttgag ccgacggtac ttcttctgat
    22561 tttcagagtg tgaggttcga aaacgattca tgatctggac acgtaattga ttgaagctgc
    22621 gattgatgtg ttggacaata tgaaagcggt cagttacgat ctccgcattt ggaaagactg
    22681 ttttaagcaa ctgacaatag ctggcattca tgtccatcac taggtacttg acctttaaac
    22741 gggcctttct aggaaagcgc aggaagtatt gagttagttt gtaaagtctg cgatcttcaa
    22801 ggatcgtaaa tagctgattg gttgtcccat caacacaaat aaaactcatg gaaccagaaa
    22861 cagacttcat tgatttgaac tcatccatac acaacaccgt cggaaggaag ttcatacgtg
    22921 gctggtaatg cttcgttgct tcacgcaaga tccgtgtcac cgtcccatcc gagacgaagt
    22981 acttcttagc gattagttta cgtgattgat tttcgtacag ttccatgaga atcgcctgct
    23041 tcagttcctt tgttagttga tgatgttcat caactaagtc cgttttcgca gaaaaggtcc
    23101 gtccacaatc atggcagaga aaacgggtac gttttaattc taaaatagtc aacttttctt
    23161 tgactttcaa catctgagtg cgtgtcgtat aggaaccgtt gcggataata cgaatcgatc
    23221 cacagtgttc acaatgggaa ggtgagtcat ccaatctccc tttaataaac tgcgcttgaa
    23281 cgcctttata ttctttttca atcagccatt cttcatcaaa aaataaatgt ttatctgtta
    23341 atccgagtaa ttttctagta tgattatcca ttgagagact cctttggtag ggtgacgttt
    23401 tggtcgacta aattttacca aaagtgagtc tctttttgag ttgaaacgaa gaaaaaagtg
    23461 ttagtagaaa atcgctttcg attttccacc aacacaaaat attatagaac cataaaaatt
    23521 tcaggacaaa tacacatagc tgtttctttt tattgtattc cttcagcaat ccaacgagat
    23581 ttatagcgaa agtcttttaa caactcttct tttgtccaat tatttgcaat gagatccgtc
    23641 aaagtgttca ttacgagggt catggaaaag tataggaaac taactgcttc ttccgacgat
    23701 tgaatggaga aatgattgat tcttaataat tccagccatt tttcgttcat tggagaatca
    23761 gccgatagtt ctctacgttt ggttgtctgc tgatcatttc gctgggttaa caagtataac
    23821 ctttgccaat agatactttc gtgatctaag tcgcagcacc actctaaata tttttcgatc
    23881 cccaaaaaga aatgttgttt gttttcgcta atgaatttta agatatcttg atggactatg
    23941 tgagaactgc ttttgatcgt atacaaataa gcatcttcca aatcttgaaa atatttataa
    24001 aaagcaccgc gcgacatccc catcttgtca acgatttctg agactttgac ttggctgatg
    24061 tgctgtgagc aaaatatctc cagtaatagg tcatcgattc tctgtttctt tgcgttcgat
    24121 aaattgaaaa aagtttcttt tggcatccac atctctcctt tatctctgat tatacgtgga
    24181 tagcccttca ttgtcatgaa ggattcattc cttctttttc tcttcttctg tcaagtaccc
    24241 atcttccatt cgataaacgc gatcactcca ttgaatcata cggacatcat gagtaaccat
    24301 aatagtcgct ttttgtttct catgagcttc tttaactagt aatttgacga cttcatatgc
    24361 atgatctgta tccaaactag ctgttggttc gtctgctaag atcaagcttg gctcattata
    24421 taaagcgcga gcaattgcca ctctctgtct ttctccacca gaaaggtctt taggataact
    24481 atttttaagt gactggatat ctaaagattg cagcaaggaa tcgatttttg ttgaatcttc
    24541 tttttttgaa gtaacttggt caactagttc aaattgttcc tttaccgtta aaaaaggaat
    24601 caaattagag ctttggagaa taaatcctat ttctttaaag cgtaaatctg ctcgtttttt
    24661 ttctggaagc tcagttagtg agtgaccgtt gatcactatt tctccggaag tcggtgtttg
    24721 caatcctcct gaaatggtaa ggaaagtact tttacctgaa ccagaaggac caatgatgct
    24781 gacgaattct ccttccttta cataaaaatc aattgctttc aaagctgaga cctttgtatg
    24841 accagaacca aatgttttgg taactttttt catcgttaat atttttttca tttttattaa
    24901 cctcctattg ctgtaattgg gtctactttg gttatcgtac gtatcgaaaa caatccgcct
    24961 agcattgcga caatgatcaa aatcccacta tatagcaacc attgtgacca aaaaacagca
    25021 aaaggcatcg cgcttggtaa aatcagacta gtcaaataag caaacaaaag agcaagcaga
    25081 acgcctacca ccccaacaat aaaggactgt gctactaaag atttagcgat gaagaaattt
    25141 ttgatccctt gtgctttcat tactccaaaa atagcagtct tctgaagtgt catcacgtac
    25201 atgaaaatac cgaccacggc agcaaccact aagaacaaaa agtatatcat cgcatttaag
    25261 gtcatatttt gggcagaata gccaggaata ctttcaatga atgtcccaat agttaataac
    25321 tgtagaccct tcgatgcttt ttctttcgaa attttagctg aatcctttat tacgatccca
    25381 ttgatcggct gttcttcttc agaagcaaac ggctgttgtc catacttcaa agcagtccac
    25441 gtatctaggg acgcatagat gacgggagaa actgtgtaat aagtttcagg aaagataccg
    25501 acaatcgtca aaggatcgtc gtaactgcca attttgatcg tatctccaat tttatagcct
    25561 gaatcagcca gattttgaga gatgatgatt tcattctttg ttttaaattc atgccctttc
    25621 gtgagttttg gtaataaaaa tgcttctttc gttgtgccga aaactgtaac attttctttg
    25681 ttttttcctt tgattgctcc gctgtacaat ccgattggtg ctttctctcc tccttcaatg
    25741 tatttcagat ctcctcttgt taattgtgaa gctgcaaata cttgatttgc atcctccgac
    25801 aaaacaatct cttgtgcgtc ccaatcatcg attgcttttt taaactcttc tgaaagtcca
    25861 ttcgctagtc ctgacaacat gaatacgaca tacgcaatca agaacataat accgacgatc
    25921 aagccatatc gtaatttaga atagcgaatt tctttgatag ctagaaacat ttgtttttcc
    25981 tccctatgat agattgatag tgacaaaatg tcacttgttg aatagtgtaa ctctaaagtg
    26041 acaatttgtc acttgtgttg tttttttctt atcaataaat tgtctaagac atctttcgat
    26101 aaaaccttaa catgagtcat aaaaaattta tttaagcctt atttatgctt tccatgataa
    26161 actaaagtga ccaaatcaat gaggtgggaa aatgattaaa ctattagtag tagaagacga
    26221 taaagtatta tccaataata taaaagaaat actcgctgca atcggcgaga taacacaagt
    26281 ttatgatggt agtgaaggac tgttcgagtt ggaaagtgga gtttatgatc ttgcaatctt
    26341 ggatttgatg atgccggaaa tgaatggata tgaagtgtta gcaaaaatac ggaaagaaaa
    26401 aatacagact ccagtattga ttttgactgc aaaagatagt ttggaagata agattgaagg
    26461 atttgaacga ggtgcagatg attatctaac aaaacctttc tatcgagaag aactgatcat
    26521 gcgcgtgaaa gcattattga aacgctcgct aggtctcttt gatgaaaatg ttctgaagta
    26581 taaggatatc agttgtgatt taaaaagcaa tgaagtcttg tataaagaac aagttttgcc
    26641 catacaagga aaagagtttg aattgctcgt ttatttttta caaaataagg gaatcatttt
    26701 gacaaaagag cagatatttg accggatctg gggatttgat tctgatacga cgattacagt
    26761 tattgaagtt tatatgagca acttacgaaa acatctgcga agtactggat tggataaaga
    26821 aatcaaaacc ttacgtaatg tcggttatat actgcaaagg gtgtgatgca atgaatggag
    26881 atattacacg aaagcaacgt agtcgctttt ttgcgacaaa tgtagcagca tttgctttta
    26941 tcttttttct tttgggattc attactttac agatattaaa ccaatctgca tacagagaaa
    27001 cagatctgtc attagagaaa ataaattttg attctcgaac agtacaatta gagatacaaa
    27061 gatatcagca aggcgatccc tttttaaacc cgactcaaac ggaacaaaac agaacaaaca
    27121 tggaacagcc attaatggaa ccatcaaatg accgttttaa cactcaagtg atcttgtggt
    27181 ctaaaaatgg tgagatattg aataaagcag ctattggtgg gcgtttcaac caaatcaaag
    27241 aattaaaaat ggatacgaaa aatctgaata ccatccaaaa tattgaatta aatgaagaca
    27301 gtggctcatc tgccctctct ttccgttcga ttacgaaaaa agcggagacg aatcaagatg
    27361 gcgttgccta catccaaatt ttagaaaata caaatcaaat caagaattca ttagcgactt
    27421 ttcgcacgat tatgattttg tgtatgattg ttttttggat tctctctatt ggtatcagtt
    27481 attacttgtc tagtctaagc atgcgcccta ttattctttc ttggagaaga caaaaagaat
    27541 ttgttgagaa tgcttctcat gaattgcgca caccgttaac gattattcaa aatagtttgg
    27601 aacatctttt taccaagcct aatcatacta ttattgaaga atccgaaagc atcgctcaag
    27661 cattgtcaga gacgaggcgc ctcactggat tgacttcgga tttgctgaca atcgctcgga
    27721 atgattctga tcagcagctg ctttcaaagc aaatgatcaa tacacaagaa tatatcgaac
    27781 aacttgttaa accatttcaa gagatggcca tcatagatgg aaaagagttt attctccata
    27841 acgatgcaac attagaagta ccaattgatg aaaaaaaaat ccatcaagtt ttggttattt
    27901 tgctggataa tgcattaaaa tatacgaatc cagaagataa gatcattttg gattcatctg
    27961 ttaaccataa agaatggctg attcacgtaa agaatactgg cccttctatc agtgaagaag
    28021 atcaagcaag gatttttgat cgattttatc gggaagatca atctcgggcg aaagagactg
    28081 gcggatacgg gcttggcttg gcaattgcaa aacaggtaat cgaacagcat catggacaga
    28141 tttctgtatc caattatctt ccacaaggag tcgattttaa agtccaattg ccgttgaagc
    28201 ctaaaaaata atgcttatcc ctaaaaaagg gctgtgacaa aaatcttgtc acagcccttt
    28261 tttgttgcga ataaacggtg ttccagaagt agcttcttcg gaaataagcc gaaatttctt
    28321 taagctgacc acttctgtca caacctcttt agcttacgct tacgattttt tattgcggtc
    28381 ttcaatgtgt acatcaccgc ttgtgtcaat atccaattct tctcggctaa catcttctgt
    28441 cactttttca acatcttcat gttcttcttt atgaatccca acttcttcac gaataactgt
    28501 atgtttgctt acatctactt gctcttcttt gattgggata gtaatcgtgt catcttccaa
    28561 tttttcgtct gttccctgtg aagattgatc tgttactggt ttgcgttcga tgacgatttc
    28621 ttcacgtttg accggtactt ctaccgtttc agtatcgttg accacatgtt tgtggatgtc
    28681 gacttctcca gttgtcacat catgtgtatt tacatctaat ttttcttctt ttagttgaat
    28741 tgtatcatca tctagatttg gtttgcgatc gatgtcttca gtcttgcttg tacccattgt
    28801 atcagtctcg ccaggtacgt ccatcggatc agttcctggt gtcatattca atggatccgt
    28861 tgtattttga aagcctgtcg tgccacctac gtccattgga tctgttccag tgccagtttc
    28921 taatccagtc cccattgtgc ttgctgggct aacagtatct tgtgtatcca ttgtatcttc
    28981 tgttgtttct ggctgacgat agccttttac tgtgatgaca aattttccat cagaaatatc
    29041 tcctctatat tgatataaag gatcattttc tggtgtagcc gtctcagaat cataggtatc
    29101 aaaagaaaaa gtatctttta ttttttccca taaagaacgg tcttcttcac catccatccg
    29161 attttcatct gtttcaacat ctataccaga caatccgtca aatcctagtc tagatgctgc
    29221 ttcatcagta gtataaataa cgatatcatc tcgttcatac ccttcgttac gcaaccgttc
    29281 aattactgat aatgcttctt ccctcgtact atagcttccg atgaccgtta aatttttctc
    29341 ttccatgtaa attcctccta gcaaatagat
//
LOCUS       DAZKLM010000026        28990 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3764
            SAMEA115706913-rid22582563.denovo.026, whole genome shotgun
            sequence.
ACCESSION   DAZKLM010000026 DAZKLM010000000
VERSION     DAZKLM010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706913
            Sequence Read Archive: ERR13196647
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 28990)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28990)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287823.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287823.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,004
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,849
            CDSs (with protein)               :: 2,849
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 41 of 96
            Pseudo Genes (incomplete)         :: 52 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 15 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28990
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706913-rid22582563.denovo.026"
                     /isolate="VRE3764"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            134..451
                     /locus_tag="ACG842_001069"
     CDS             134..451
                     /locus_tag="ACG842_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HFQ0247629.1"
                     /translation="MLFYSNFILIVAILLLLNIWIFDRSRNASIGFRTKRSLSSKKNW
                     VYSQTIFYGGIVLISLLSSTLYSLNIIDVSTSNSISIIGIIIAAIITQLFLVFGEKKR
                     SKK"
     gene            complement(655..1020)
                     /locus_tag="ACG842_001070"
     CDS             complement(655..1020)
                     /locus_tag="ACG842_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0247630.1"
                     /translation="MIKTCWKNLPLLLSFVPYVHFALLLDFRYHSVSGFITLIFLSLF
                     AGYYFQRNRRIISLFIANIISTVTSYLFCANFTEWRYFYHPLKPTQLILLLAGIYLVP
                     QILGSLWAVALSYKKARHP"
     gene            1228..1710
                     /locus_tag="ACG842_001071"
     CDS             1228..1710
                     /locus_tag="ACG842_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0247631.1"
                     /translation="MRRKAVIDRLYLFFWFFPIVLLPFTWVNAVVQGAEILGLIQGSG
                     FVVSRNFPALTVFYLVISVGLLLPKSLINHYNWQLLYFSCLLVFTSMFSLLPRLIIGP
                     NGLSESGLKDSAYSSMYFLTIKPWFYLVIASFILATFFYFWTEIRIKSERSVSIKNNY
                     "
     gene            1907..2797
                     /locus_tag="ACG842_001072"
     CDS             1907..2797
                     /locus_tag="ACG842_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042301 - phosphate ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS family protein"
                     /protein_id="HFQ0247632.1"
                     /translation="MKKVLFTLGMMGILLAGCGSGSGNTDGSATQNNSSDSNEQVKIV
                     AVGSTALQPLVDAAQESFVQENPNYQISVQGGGSGTGLSQVEAGAVTIGNSDVFAEER
                     DGVDASKLVDHKVAVVGMAPIVNKDTDVKDITKQELIDIFTGKITNWKEVGGKDQKIN
                     VVNRANGSGTRATFEKWGLDGATPVQSQEQDSSGTVRQLVSQTPGAISYLAFSYLDDS
                     TQALSIDGVEPKEENVADNSWGIWSYEHMYTNGKPSPEVQKFLDYMMTEEIQEGPVKE
                     LGYLPITMMEVERDHEGNIK"
     gene            3122..5593
                     /locus_tag="ACG842_001073"
     CDS             3122..5593
                     /locus_tag="ACG842_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HFQ0247633.1"
                     /translation="MKNKWINRSKKKKVTQRSSDKVIRLFPSYKEGLTADQVKERIEK
                     GAANNSVDPTFKTNQQIVLENIFTYFNLIFLILAILLCLVESYKNLTFLPVIIANTGI
                     AIFQEIRSKKILDELNVLNTAKVNVIRDGIEQTIDIEDLVIDDIVILETGHQIPADAE
                     VVEGKLQVNEALLTGEADEITKEIGDPLMSGSFIVSGKAYARLDKVGADSYISQLTVK
                     AKTMGEGEQSEMIASLNQLIKWIGIIIIPIGILLFSQSYFFNGNTIKESVVAMEAALI
                     GMIPEGLYLLTTIALALSATKLAKQRVLLHNMKSIETLARVNVLCVDKTGTITENKMS
                     VQKVIVSKKQEIISQSDQLEACIADYAKAMAGDNATMEAVKAYFTKTTDQSYHHVIPF
                     SSVQKFSSVALADKVYVLGAPEMVLRDQLPEYAEEFVSFAEKGYRVLVFGVYDGILEE
                     PVLTEAVTPLGYILIANPIRKEARATFDYFKKQQVAIKVISGDNPLTVSNVAVQAGIS
                     HADQYVDASKLSEEEYEAAVEKYTVFGRVKPEQKKIFVQLIKRNNTVAMTGDGVNDIL
                     AMKEADCSIAMASGNEAAMQASQVVLLDSDFSRMPEVVAEGRRVVNNIERSASLFLVK
                     NIFSFLLSLFSVIFALTYPLEPSQITLISLFTIGLPSFLLALEENKKRIRGKFIMNVM
                     EKAVPGGLTDMIVVGALVICGVTLNLNKTDVSTASTMLLIAVGFLVLYKICSPLNKFR
                     SQIILFCASGNFFSVIFLHKLFSITGISAVSLLLLSILFFSADSIFRHLTTLVEYLFE
                     RKNEKEQKPFSRVAKKFLSIFKREN"
     gene            complement(5696..7432)
                     /locus_tag="ACG842_001074"
     CDS             complement(5696..7432)
                     /locus_tag="ACG842_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFQ0247634.1"
                     /translation="MKWKKIYERLFFLILLVSLFFGGWQLISHYFQQQIVEQQESYLT
                     KKAQLLIRQLDVENLQAAQNKTALEEFVHQSNERITLMDATGKILFDTNNETLNEQRN
                     SRPEIKAVLNGGNLGSALRKSTTLNEDLLYVALPVKKSGQLEAILRIAEPTSGFLPRT
                     ESFRRWVFFFFLSFFLVLAGMIYYLIYQKNQPLKTVLPVLKKMVQNPNQAEVIMQTPD
                     QWEELYQTINALSEQMSKMYRACTTTEEQLYTLLNELMIGVFLIDDADSRLLLLNPKM
                     QLHLNVVSYQPEQRYTEVIQEPKLIQLIHQVTPEHPLIHQEITLTEGRQTLDLSLRYF
                     TNSEGTGQILGVAYDLTKVRRLEKLQKDFVGNVSHELKTPVTSLIGFTETLLDGAKDD
                     PQTLTSFLEIMQKDAIRLDKLIREIIQLSKDGENSYEIQTIYTEPYFQQIVQNYQPII
                     EKKRLTIRLIGKNEPFTTKTDILYPIIKNLIENAVQYSKSDSEIIIRYQATDDLSFSV
                     QDFGIGIDIEDQERIFERFYRVDKARSRHSGGTGLGLAIVKDYVQLLNGTITVDSHLG
                     TGSTFTVTIPKM"
     gene            complement(7429..8133)
                     /locus_tag="ACG842_001075"
     CDS             complement(7429..8133)
                     /locus_tag="ACG842_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFQ0247635.1"
                     /translation="MKKILVVDDEPSIVTLLTFNLEKEGYKVTSATDGGEGLELALEQ
                     SFDFIILDVMLPTMDGMEITQRLRQEKNETPILMLTAKDDQVDRIIGLEIGADDYLTK
                     PFSPREVLARMKAIFRRIEPRKQSKDEAEPEYLSIGQITADLTNYQVTVDDHLIELTP
                     KEFELLVYFMKRKDRVIDRDTLLDRIWNFDFAGQSRIVDVHVSHLREKIERDPKHPKY
                     LVTIRGFGYKFQEPKR"
     gene            8264..8767
                     /locus_tag="ACG842_001076"
     CDS             8264..8767
                     /locus_tag="ACG842_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HFQ0247636.1"
                     /translation="MIGISACLGGLACRYDGQKKEVPELMELVETGEAVMVCPEVIGG
                     LPIPRDPAEIIGGNGFDVWKDQAKVWTTSGEDVTEAYKNGAIKAYQKLQEKHIDLVIM
                     KEKSPSCGTHSIYDGSFSGIKKNGAGVATAYFIQQKMTVLSDEEWLKTRGEQNGNRKD
                     QSNERAL"
     gene            8727..9065
                     /locus_tag="ACG842_001077"
     CDS             8727..9065
                     /locus_tag="ACG842_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HFQ0247637.1"
                     /translation="MEIEKTNRMNALFEFYSTLLTEKQMNYMELYYADDFSLGEIAEE
                     YEVSRQAVYDNIKRTSKILEDYEKKLHLFSNYIVREQVLENMTAYITDKYPEDKKLLE
                     YVQQIQAIEE"
     gene            9086..10504
                     /gene="ffh"
                     /locus_tag="ACG842_001078"
     CDS             9086..10504
                     /gene="ffh"
                     /locus_tag="ACG842_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HFQ0247638.1"
                     /translation="MAFESLTERLQQAMGKIRKKGKVSEADVKEMMREIRLALLEADV
                     NLQVVKDFTKRVRERAVGAEVLESLSPAQQIVKIVDEELTITLGSETAELNKSPKIPT
                     VIMMAGLQGAGKTTFTGKLANYLKKNENARPLLIAGDVYRPAAIDQLKVLGQQLDVPV
                     FDMGTEVSPVEIVRQGMELAKEKKNDYVLIDTAGRLHIDETLMDELKQIKELTQPNEI
                     LLVVDAMTGQDAVNVADSFNQQLGITGVVITKLDGDTRGGAALSIRSVTGAPIKFIGS
                     GEKLTDLEVFHPDRMASRILGMGDMLTLIEKAQQDYDEKKAEELAQKMRENSFDFNDF
                     IEQLDQVMGMGPLEDLIKMIPGMNQVPGIENVKVDPKDVERKKAMVYSMTPAERANPD
                     LLNPSRRRRIAAGSGNSVVEVNRMIKQFKESRKMMQQMSKGDMNIPGMDQMFGSGVKG
                     KLGKMAMNRMIKKNKKKKKKRK"
     gene            10616..11194
                     /locus_tag="ACG842_001079"
     CDS             10616..11194
                     /locus_tag="ACG842_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HFQ0247639.1"
                     /translation="MRCPWSEQTESMRQYHDTVWGVPEYDDQKLFRKLMLDINQAGLS
                     WQTILNKSEAFDEAYDRFDIKKVANYGETKISELMENKGIIRNRRKIEAAVQNAKAVL
                     KIQEEIGSFSEYLWSFSNGKIVDSSYHSQEEVPTTSPLSDELAKDLKKRGFKFIGSTT
                     IYAFLEAVGIINDHLDSCFRKEEVVRIGRKGE"
     gene            11198..11719
                     /locus_tag="ACG842_001080"
     CDS             11198..11719
                     /locus_tag="ACG842_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HFQ0247640.1"
                     /translation="MRKIIFYGAISLDGYLATKTDDLQWLFDTPTGKQTTYEAFYETI
                     DTTIMGRKTYQEAKKYLETEAIYPEKKNFVFSTHAQLQLPDAEVVNEDPVAFLKNLQM
                     TEGKDIWVVGGGRLIKPLLEHQMIDEWYIQITPVLLGDGIPLFQKGTYEARFEMLDTT
                     RFGEFIELHYVRK"
     gene            complement(11798..12352)
                     /locus_tag="ACG842_001081"
     CDS             complement(11798..12352)
                     /locus_tag="ACG842_001081"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0052873 - FMN reductase (NADPH) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HFQ0247641.1"
                     /translation="MTKKIGFFIGSLRKDSYNKKVAEAFAKLLPEGYESVFVKIDDLP
                     FYNEDLETPEQAPAEWTRFREEVRGLDGVVFVTPEYNRSVPAVLKNALDVGSRPYGHS
                     VWDHKPGLVVTASPGGIGGFGANHHLRQSLVFLNVPTLQQPEAYIGNVANLIDENGNI
                     VEGTLSFFQTILDAYLDFSNKLTE"
     gene            12605..12880
                     /gene="rpsP"
                     /locus_tag="ACG842_001082"
     CDS             12605..12880
                     /gene="rpsP"
                     /locus_tag="ACG842_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HFQ0247642.1"
                     /translation="MAVKIRLKRMGSKKSPFYRIVVADSRSPRDGRFIETVGTYNPLK
                     DPAEVVLKEDLVLDWLSKGAQPSDTVRNILSKEGVMQKHHEAKFSKK"
     gene            12892..13143
                     /locus_tag="ACG842_001083"
     CDS             12892..13143
                     /locus_tag="ACG842_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HFQ0247643.1"
                     /translation="MKDLSDLVLTIVRPLVTYPDQVKLDVVESKDFYEYNLTVAPEDI
                     GRIIGKQGRVAKAIRTIVYGVRINAPKKVRLNIIDDKEK"
     gene            13268..14059
                     /locus_tag="ACG842_001084"
     CDS             13268..14059
                     /locus_tag="ACG842_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0247644.1"
                     /translation="MRKKMLFIPFALLLSACSSVTQESDNTHLSSSISETVTSPEKSS
                     TKTASTTEQTMATSNNEKKTALDQLKEQQPNVPMPLDVPVSSGYLNIAATHTKQGYSI
                     LYYRTDRPLGLNADELNQETPIATYLYQYGFASSQETIQVLQPFEIDTNGQQVDLGSR
                     ITGYQQGAAGSSFLEWQEGNWRIRIRGNNIEGQDPLLLAKEIVAYLEENSLPAPEQFG
                     KITVDMGDTTNRAVEVSWQEPKNAYTITHQDPMSALKMAVSMKRL"
     gene            14229..14750
                     /gene="rimM"
                     /locus_tag="ACG842_001085"
     CDS             14229..14750
                     /gene="rimM"
                     /locus_tag="ACG842_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319275.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HFQ0247645.1"
                     /translation="MTEYLNVGKIVNTQGIKGEVRVISTTDFPEERYKKGTVLTLFQD
                     GKAPVELTVKSHRKHKNFDLLSFEGHPSINDVEKYRDGILKVSKDNSVDLAENEFYYH
                     EIIGLHVLEDGTELGKVKEILSPGANDVWVVQRPKKADILLPYIASVVKEVDLEAGVV
                     RVEIPEGLIDDED"
     gene            14740..15501
                     /gene="trmD"
                     /locus_tag="ACG842_001086"
     CDS             14740..15501
                     /gene="trmD"
                     /locus_tag="ACG842_001086"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HFQ0247646.1"
                     /translation="MKIDVLTLFPRMFEGPMGESIIGKAVDKGLLDINISNFRDYSDN
                     KHQTVDDYPYGGGAGMLLKVQPIYDNIQAIEKAAPDVKKRVILLDPAGKRFDQKMAEE
                     FSQEEHLVFICGHYEGYDERIRTLVTDEVSLGDYVLTGGELGAMVMIDATVRLLPDVL
                     GNQTSAQTDSYSTGLLEHPQYTRPAEYKGMKVPEVLTNGNHKLIEEWQLKESLRRTYQ
                     RRPDLLETLEWTPQMTKFLEEIKKEEQEKTADEAK"
     gene            15637..15984
                     /gene="rplS"
                     /locus_tag="ACG842_001087"
     CDS             15637..15984
                     /gene="rplS"
                     /locus_tag="ACG842_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005590628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HFQ0247647.1"
                     /translation="MNPLIEELTKEQLRSDIPAFRPGDTVRVHAKVVEGTRERIQLFE
                     GVVIKRRGAGISETYTVRKVSNGVGVERTFPLHTPRVAKIEVVRYGKVRRAKLYYLRA
                     LHGKAARIKEIRR"
     gene            16357..17019
                     /locus_tag="ACG842_001088"
     CDS             16357..17019
                     /locus_tag="ACG842_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010740290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0247648.1"
                     /translation="MKQNDGRIIWKNQSELKLILTINEFIEKHGITSSRQYQKKLSEN
                     PNSAPSMWFINKKYGSWENLLISIGRENTGYGKWARMSEQELLEIVEAFIKCEKITSQ
                     RMYEQKSVGKNIPSLSTIKKMLGDIRPLFKEKNDGSRFTDFELLLELKNEIIRLKLQD
                     DLSMTKFRKLVQSPKLPSVDTIMKRTNKNWEELMAEIGFDYRRIKIYKQRNNLSKTKK
                     TK"
     gene            17098..20325
                     /gene="fss3"
                     /locus_tag="ACG842_001089"
     CDS             17098..20325
                     /gene="fss3"
                     /locus_tag="ACG842_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
                     /protein_id="HFQ0247649.1"
                     /translation="MKKPRFKNWRLLATLSLLCQTIGGSLGPTIAFADEITHPQTVTV
                     ELDLAHQYAVEGTFSDGRPMSEVTVPHYAVYNGVKQDIFCIEPGVPIYNEFTPGYEKN
                     PLPDMSEKAKLVSVLWKEAGTDVDTHIVAQKMIWQEVNGYTLHSIKRSDGSAVNIAAI
                     ESKINQAIADYQKKPSFHNSTVKTVLGQSTTVTDTNGLNLSEFDEVVENIANIDYRVN
                     GNQLVITPNANSKESGELTLKKSANTGTPVAYKMAGQQTLMAGAIDKPNTYTIKIDVE
                     TEGSLKIKKVDKETGNVVPGTVFHLDFGKTLPAKDVTTDKEGIATLDEIPHGAKVTIT
                     EKSVPAPYTIDTTPITATIKSGETISVTSKNTREKGQIILNKMGVETGTDLWNDKYSL
                     AGNTFAIRKDSPTGEIVQEMTTDEKGHAETPKEIANALELGTYYVTETKASNGFVNTF
                     KPVKVELNYVNQTVALVTSNVKGQNQEITGETTLTKEDKDTGNETQGKAEFKGAEYTL
                     FTAKDGQAVKWSEAFKPELVKGTKASDETVTLTLDEKNQVAVKHLAINEYYWQETKAP
                     EGYTLDETKYPVSIKKVDDNEKNAVITRDVTAKEQVIRFGFDFFKFAGSAAGTAETGF
                     NDLTFKVSPLEGTSEITDAEDEAITAYNEQLGFDGYGKFENLPYGDYLLEEVEAPEGF
                     QKITPLEIRSTFKENKADYTKSEYIFTITEKGQKQPIKTVTVPYEKLTNKAFSVSLNR
                     LMLYDLPEEEDSLTSLGTWKDGGKELTDLDSTELVDKLSYNLHEIKEDWYVVAQAIDV
                     EATKAAQEKDEKAKPVVIAETTATLANKEKTGTWKILHKLTAEQVLDKTIVLFNYVYE
                     NKKPYEAGDDPVAKDASLNNQAQTVNCTVERHVSIQTKAHLEDGSQTFTHGDVVDMFD
                     DVSITHDVLDGSKEAFETILYALLPDNTTKEIWKSGKIDYEVNDKEFTKTVLAEKVDT
                     SKYPEGTSFTFKEINYGKDGNINGKHNEDLKEKTQTLTPKKVPTTPSAPEQPEIPTVP
                     SDSQESSPTVKTFPQTGEKNSNVLLFIGFTLIFATAGYYFWNRRN"
     gene            20439..20753
                     /locus_tag="ACG842_001090"
     CDS             20439..20753
                     /locus_tag="ACG842_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HFQ0247650.1"
                     /translation="MELKFVVPDMAETFGKIRYAGEGEVLTEGYGRNTTVIGRSYHLY
                     SSKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLVAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            20766..21140
                     /locus_tag="ACG842_001091"
     CDS             20766..21140
                     /locus_tag="ACG842_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HFQ0247651.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTVSNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIMDTVANYVFREGTTVNWFIKA
                     DDLVLKRQPDQGNSTNQNEGKK"
     gene            21150..21494
                     /locus_tag="ACG842_001092"
     CDS             21150..21494
                     /locus_tag="ACG842_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HFQ0247652.1"
                     /translation="MEINVEHILDCLDQYGKGELTEEQLTKALTYDEKMFLIMHQGLL
                     EVGNDAEEDFDVLNWLGEEESFFMVIEVNESLCREAETVLEEIGVEMPDAIEVFLKQL
                     VETKQLPIAVND"
     gene            21565..22917
                     /locus_tag="ACG842_001093"
     CDS             21565..22917
                     /locus_tag="ACG842_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsK/SpoIIIE domain-containing protein"
                     /protein_id="HFQ0247653.1"
                     /translation="MYKGHRIRAGDQHLVYHFVLGWLLALFIGWMSVFYFQELRQFDI
                     SKLSLSTIEIVRSIKDLIYLLGSLVLSGSTMLLYIHFFQDHWRSLWHRQKLARMILEN
                     HWYEVKQTQSEGFFKDLNSSRTKETISYFPKIYYRMKDGLLSIRVQISLGKYQDQLLK
                     LEKKLESGLYCELVEKELKDSYVEYTLLYDIIANRIGIDEVVAESGALRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIEALLKSDAELFVLDPKNADLADLGTVMPHVYSQK
                     EEISACVEDFYERMMTRSKAMKEMPNYKTGENYAFLGLPPNFLIFDEYVVYMEMLTTK
                     ESAVILNKLKQIVMLGRQSGFFLILACQRPDAKYLGDGIRDQFNFWVALGRMSELGYS
                     MMFGEVDKKFFMKRIKGRGYVDTGGSVISEFYTPLVPKGYDFLREISDIGGLNVNTEG
                     "
     gene            22977..24119
                     /locus_tag="ACG842_001094"
     CDS             22977..24119
                     /locus_tag="ACG842_001094"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HFQ0247654.1"
                     /translation="MTFLDLFAGIGGFCLGMEQAGHQCIGFCEIDDFARQSYKAIHDT
                     SKEVEMHDITSVSDEFIQSLGPVDILCGGFPCQAFSIAGKRQGFSDTRGTLFFEIARF
                     AAILQPKFLFLENVRGLLNHEGGATFETILRTLDGLGYDVEWQVLNSKAYVPQNRERI
                     FLIGHSRDACTEQVFPIIGSSPTSDQNIRNLLNINPSNRGMGGQVYGSDDVAPTLTGD
                     EGIKIALPVDDGISVAGMLLGNFEQGNRVYEITGTAPTLSTKQGGTKIMLRKNETEYQ
                     EVKPGNSVNLAFPNSTSRRGRLGKQSVHTLLTGDQQAVVTDQYQIRKLTPRECWRLQG
                     FPDWAFDRASQVNSDSQLYKQAGNSVTVPVIFDIARRLKEVKTDDL"
     gene            24144..24398
                     /locus_tag="ACG842_001095"
     CDS             24144..24398
                     /locus_tag="ACG842_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0247655.1"
                     /translation="MIGKIQSDPELEEELYRLNFDIRKNGVKVPDDPYVIDEETDARM
                     ELNQVISELERIADFAKEPDIRQYLFEIKAELEIEGITAE"
     gene            24654..25838
                     /gene="mobT"
                     /locus_tag="ACG842_001096"
     CDS             24654..25838
                     /gene="mobT"
                     /locus_tag="ACG842_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MobT family relaxase"
                     /protein_id="HFQ0247656.1"
                     /translation="MAQRNLDYRLLKDRRNEYGVSQNKLATTCGLSRPYLNQIENGGV
                     TASTKTMRKIFDQLESFNPELPLTLLFDYVRIRFPTTDARKIIQEILHLKFDYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCL
                     KAGGVMKRLDLAINDRVGLLDIPDLTKKCQKEECISLFRTFKSYRSGELLKPDDKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAYHAIQDLL
                     KGRSIESTTFSIINRYLRFADKVEGKRRTNWPLNEQWGRFIGRNQKEIQLTSEPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNQTTYIQDMVNNTRLSDRHKKILEQQSMAIENL
                     II"
     gene            25835..25972
                     /locus_tag="ACG842_001097"
     CDS             25835..25972
                     /locus_tag="ACG842_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HFQ0247657.1"
                     /translation="MKRGIVMEIVKDILLVFGGSMLGVTVMCLMHASAAADRAMEKKG
                     K"
     gene            25977..26198
                     /locus_tag="ACG842_001098"
     CDS             25977..26198
                     /locus_tag="ACG842_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0247658.1"
                     /translation="MNFGQNLYQWFLTNAQSLVLLAIVVIGLFLGFKREFSKLIGFLV
                     IALIAVGLVFNASGVKDVLLNLFNRIVGA"
     gene            26211..26711
                     /locus_tag="ACG842_001099"
     CDS             26211..26711
                     /locus_tag="ACG842_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HFQ0247659.1"
                     /translation="MEQIRVYIANLGKYNEGELVGAWFTPPVDFDEVKERIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCGLAEELKGTPIGEVASEIQHAFFNSFEEMVEH
                     VDDIIYYPDCNDMSDVAYYLIVEAGDLGEVPEHLKTYIDFDACGRDLEIGGNYLVTSR
                     GVFELM"
     gene            26808..28655
                     /locus_tag="ACG842_001100"
     CDS             26808..28655
                     /locus_tag="ACG842_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HFQ0247660.1"
                     /translation="MVGNKWYKCDLHIHSTASQCFQDKKVTAKEWVEECLNKGLDCVA
                     LTDHNTGENIDEYKKVAIENGLDFFPGVELTCGENGTHLLVLFDKSACTNDISDFLTK
                     MGIKREDWASAAACSMKSVLEVIDFAEHEGLLVIPAHIDEFNGISSAKFGNLGKIFES
                     ENIYAVQTVQKEFFENRSQMIPSSKRDTIYDSVNARYDGRVGKDTLESWYKSVVEAEK
                     NNMTFLSFSDNPHSKGNSKHGLWGIGTRYSYIKMKDEPDLGSLRDALMLGETRVHSDF
                     SNFSINENEVLLEKLSFSGTTLSTKEVVVEFSDNLTSIIGGRGTGKSCITRFLVYVLG
                     KEAELDQFSEIQSDYQNFAQIEHNGSGIFLKDTIVKLNIFYKGTKYQIIRTQDRHSIY
                     EFTQENGLVEKETERLRMISDKVSIYSQKQIYEISKNQTSILELLDGYNSDLISEYKN
                     EIETCVNEIRKLNWDIVSVKKEIQDKAKVELEIEDLKKQVEKLSHKSYKDVYDEYSKE
                     TDIYRELKRDVEALKEIPKNLTDSVDKIDFGNGIKVTDEIDEHRGELIKNLVTNRAKI
                     QTIINEMSDEIDSYRAELNKSDWKVNYNEVSKRYQRGIKNLVKSYLKMN"
     gene            28658..>28990
                     /locus_tag="ACG842_001101"
     CDS             28658..>28990
                     /locus_tag="ACG842_001101"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0247661.1"
                     /translation="MLQNLDSLYQSLSKTEERLEEIIQQEIKLKDYQVEVDSAMSRMN
                     KSYQNFYSERSSSSREIFSDITGIEATIQPNRDFNNYVKELRACINKEDTFDSEFGSL
                     IDKLTKNSI"
BASE COUNT         9744 a         4933 c         6166 g         8147 t
ORIGIN      
        1 tggtaactat gcactgtaac gataatgctg ctaaaatatc tttttacgaa aaacatcaca
       61 aggaaaaact taactatggt attatctaaa cataattttt tcggtattta atatttaatt
      121 aggagggaat attttgcttt tttatagtaa ttttatctta atagtagcga tattattact
      181 tctgaatata tggatttttg ataggagtag aaatgcaagt ataggtttta gaactaaacg
      241 aagtctgtct tctaaaaaaa attgggtata ctcacaaact attttttatg gaggaattgt
      301 tttaattagt ttactttctt caacacttta ttctttgaat attatagatg tttccacctc
      361 aaattcaata tctattattg gaatcattat tgctgcaatt attacacaac tgtttttagt
      421 gtttggagag aagaaaagaa gtaaaaaata gaaaaactag tgatagtttt ggtgatagtg
      481 atattaaaaa tcagaaaaga tagaaaaagc aggagataga taaaatgctg atatttcagc
      541 attttttaag agtataaaag agaaaaaaag taataaatgt atacatgata taagaaaaac
      601 atatatgtat aaaaagaaga aagtagcttc tctttgtaca tatatggttt gtttttatgg
      661 atgccgagct tttttataag ataaggctac cgcccataac gacccaagga tttgtggaac
      721 taagtaaata cctgcaagaa gcaagatcag ctgggtcggt tttaaaggat gatagaagta
      781 acgccattca gtaaaattgg cgcaaaataa ataggaagtg acagtagata taatattggc
      841 aatgaataaa gatataatac gcctgttcct ttgaaaataa tatccagcaa aaagagataa
      901 aaatatcaat gtgatgaacc cgcttacgga atgataacga aaatcaagca gtaaggcgaa
      961 atgcacataa ggaacaaaag ataaaagcaa cggaaggttt ttccagcaag tttttatcat
     1021 ctttcatcac cacctaacaa gtgttattat tttgttagtt ttattgtaaa ttgaatcctg
     1081 caataatcaa ttactttttc attatttcta gattaaatga ataaactctc atgccattat
     1141 ctctaaaaaa aaagtttttc cccctaattt ttttagatag aaaattgtat agtatgatga
     1201 gagaaaaagt agtttggggg aacgttaatg agaagaaaag cagtaatcga tcggctttat
     1261 ttattttttt ggttttttcc tatcgttttg ctacctttta catgggtaaa tgctgttgta
     1321 caaggtgcgg agatattagg cctgatccaa ggaagcggat ttgtggtttc taggaatttt
     1381 ccagctttga ctgttttcta tttagtgata agtgtaggtc tcttattacc aaaatctctg
     1441 atcaatcact acaattggca gctgctttac ttttcttgct tattagtatt tacatctatg
     1501 ttttctttgc tgcctcgctt gattatcggc cctaatggtt tgagcgagag tggattgaag
     1561 gattcagctt attcctccat gtatttcttg acgatcaaac catggttcta tttagtaatt
     1621 gcctcattta ttcttgcaac atttttttac ttctggacag aaatcaggat aaaaagtgaa
     1681 cgatctgtat ctataaaaaa taactattga tggtggatca tcagtaaaaa agcatatgag
     1741 ctgcgtaaaa tgcatgtttc tcatatgctt tttttattat tatgtaaaga atgcgaaaaa
     1801 cgtttacatg atttttacat aacttaacta tatttttaca gcaaccatac gagggattta
     1861 cagtcgactg ataaacttta gttgtaaata agaaaggagc aataaaatga aaaaagtatt
     1921 gtttactcta ggtatgatgg gaattctttt agccggatgc ggctcaggat caggaaatac
     1981 agatggctct gccacacaaa ataattcaag tgactcaaat gaacaagtaa aaatcgttgc
     2041 ggtaggttcg acagcattac agccgttagt cgatgcagca caagaaagct ttgtacaaga
     2101 aaatccaaat tatcaaatct ctgtacaagg aggaggaagc ggaactggtt tgagccaagt
     2161 cgaagctgga gcagttacta tcggaaactc tgatgtattt gcagaagaaa gagacggagt
     2221 tgatgcttca aaattagtgg atcataaagt agcagtggtc gggatggcgc caatcgtcaa
     2281 taaagataca gatgtcaaag atatcaccaa acaggaattg attgatatct ttacaggaaa
     2341 aatcactaac tggaaggaag ttggcgggaa agatcaaaaa atcaacgtcg tgaaccgtgc
     2401 aaacggcagc ggtacccgcg cgacattcga aaaatgggga ctggatggtg ctacccctgt
     2461 gcagtcccaa gaacaagatt catcaggaac agttcggcag ctggtaagtc aaacaccagg
     2521 agcaatcagc tatttagctt tctcatatct cgatgattcc acacaagcac taagtatcga
     2581 cggtgtagaa ccaaaagaag agaatgtagc agataacagc tggggaatct ggtcatatga
     2641 acatatgtac acgaacggaa aaccttcccc tgaagtccaa aaattcttag actacatgat
     2701 gacagaagaa atccaagaag gacctgtcaa agaattgggc tatttaccaa tcacaatgat
     2761 ggaagtagaa cgtgatcacg aaggaaatat caaataacta gcagataaac atgccacggt
     2821 agtcgttact attaaggaaa aatcaactat gtttcccaca tatacatttt tcctttaatt
     2881 gtgaaaaata gttcctcata ttttgccgtt ttttgagcta ataccgaggg tcagctgatt
     2941 aaacaccgtt tattcgaaaa aaagagctac gacatagaac tgtcgtagct ctttttttgc
     3001 tatatgacag gaatgaaagg tgtttgcaag cttaatgtga ggaacacttt ccatatctgt
     3061 tctatactgg ataggagaag tttggaaata tccagaaaac gaaatcaatc gaggtagcca
     3121 catgaagaat aaatggataa atcgaagtaa aaaaaagaaa gtaacacaaa gatcatcaga
     3181 taaagtcatc cgtctttttc catcgtataa agaaggatta acggcagatc aagtcaagga
     3241 acgaatagaa aaaggtgcag caaataattc agtcgatccc acattcaaaa caaatcagca
     3301 aatcgtattg gaaaacatat ttacgtattt caatttgatc tttcttatct tagccatttt
     3361 gctttgttta gtagaatcct ataagaactt gactttttta ccagttatca tagccaatac
     3421 aggaatcgcc atttttcagg aaattcgatc caaaaagatc ttggacgaac taaatgtttt
     3481 aaatacagca aaagtcaatg tcatacggga cgggatagaa caaacaatag atatagaaga
     3541 ccttgtaata gatgacattg ttattttaga gactggacat caaattccag cagatgcaga
     3601 ggtcgtggaa ggaaaactgc aagttaatga ggctttattg acaggcgaag cagatgaaat
     3661 aaccaaagaa ataggcgatc cattaatgtc cggcagtttc attgtttccg gaaaagctta
     3721 cgcccgatta gacaaagttg gagcagattc gtatatttcc cagctgacag tcaaagccaa
     3781 gacaatggga gaaggagaac agtcggagat gattgcttct cttaatcaat tgatcaaatg
     3841 gattgggatc atcattattc ctatcggtat cctgcttttt tctcaaagtt acttttttaa
     3901 tggaaacacg atcaaagaaa gtgtcgttgc aatggaagca gcattgatcg gtatgatacc
     3961 tgaaggactg tacttgctga cgacgattgc attagcatta agtgcgacta aactagcaaa
     4021 gcaaagagtt cttttacaca atatgaaaag cattgagaca ttggcaaggg taaatgtcct
     4081 ttgtgtagat aaaacaggaa cgatcacaga aaataaaatg tctgttcaaa aagtgatcgt
     4141 ctctaaaaaa caagaaataa tcagtcagtc agatcaattg gaagcatgca ttgctgacta
     4201 tgcaaaagcg atggcaggag acaatgcaac aatggaagct gttaaagcat attttacaaa
     4261 aaccactgat caatcctatc accatgttat tcctttttct tctgtccaaa aattcagcag
     4321 tgtagcgttg gcagacaagg tgtatgtttt aggagcgccc gaaatggttt tgcgcgatca
     4381 attgccggaa tatgcggaag agtttgtttc tttcgctgaa aaaggctatc gcgtgttggt
     4441 ctttggtgtg tacgatggga ttcttgaaga accagtattg acagaagcag tcacaccatt
     4501 aggctatatt ctgattgcta atcctatcag aaaagaagca agagccacat ttgattactt
     4561 caaaaaacag caagtagcca tcaaagtgat ttctggagat aatccgctca ccgtatccaa
     4621 tgtagctgtc caagcaggga tttcacatgc tgatcagtac gttgatgcat ctaaattatc
     4681 agaagaagaa tatgaagcag ctgtcgaaaa atatacagtt tttggacgcg tcaagccaga
     4741 acaaaagaaa atcttcgttc aattgataaa gcgtaacaat acagtagcta tgacaggtga
     4801 tggcgtgaat gatattctag ccatgaaaga agcagactgc agtatagcga tggcttccgg
     4861 aaatgaagcc gccatgcagg cctcacaagt ggtcttactt gattctgact tttcacgcat
     4921 gccagaagtt gttgcagaag gacggcgcgt tgtgaacaat attgagcgat cagccagctt
     4981 gtttttagtg aagaatattt tctccttttt gctctcactg ttttcagtga tctttgctct
     5041 gacctatcct ttagaaccat cacaaatcac attgattagt ttgtttacta tcggacttcc
     5101 atctttcttg cttgctctag aagaaaacaa aaagcgaatc agagggaaat tcatcatgaa
     5161 tgtgatggaa aaagctgtgc caggtggact aacagatatg atcgtagtcg gtgcgttggt
     5221 gatctgcggg gtgactttga atctaaataa aacagatgtt tctacagcat ctaccatgct
     5281 tttgattgca gtagggttcc tcgttctcta taaaatctgt tcaccgctga ataaattcag
     5341 aagtcagatc attcttttct gtgctagcgg taactttttt tcggtcatct ttttgcataa
     5401 gctgttttca atcaccggta tctcagctgt atcacttttg ctgttgagca ttctattttt
     5461 ctcggcagat tcgattttta ggcatttgac cacattggta gaatatttgt ttgaacgtaa
     5521 aaatgaaaaa gagcaaaagc cgtttagtcg cgtagctaaa aaatttctat cgatatttaa
     5581 acgagaaaat tgacgaaaag tatctcaaaa aagagtaaaa aagaacaacc actcgtgtat
     5641 ctttcgaagc aggatgagtc atccaacttt aaaacgcacg agcagttgct cttttttaca
     5701 ttttcggaat ggtaacggtg aaagtcgaac ctgttccaag atgactgtct acagtaatcg
     5761 taccattaag gagttgcacg tagtctttta caatcgctaa ccctaatccg gtcccgcctg
     5821 aatgtcggct tcgcgccttg tctacgcggt aaaaacgctc aaagatccgt tcctgatcct
     5881 caatgtcgat acctatacca aagtcttgga cagaaaacga tagatcatct gttgcctgat
     5941 agcggataat aatctcgctg tcgctttttg aatattggac agcattttca atcagatttt
     6001 tgataattgg ataaaggata tctgttttag tagtaaatgg ttcgttcttt ccaatcaatc
     6061 gaatcgtcaa ccgtttcttt tcgatgattg gctgataatt ttgaacgatt tgctggaaat
     6121 aaggttctgt ataaattgtc tggatctcgt aagaattctc gccatctttt gacagctgga
     6181 tgatttcccg aatcagttta tctaaccgga tcgcatcttt ttgcatgatc tcaagaaaag
     6241 aagtcagagt ttgcgggtca tcttttgcac catccagtaa agtctcggta aaaccaatca
     6301 gtgaagtcac aggagttttt aattcgtgag agacgttgcc aacgaaatcc ttttgtagct
     6361 tttctaaacg tcgcactttc gttaagtcat acgccacccc tagaatctgt ccggtacctt
     6421 ctgaattagt aaagtagcgc aaactcaagt ctaatgtctg ccgaccttct gtaagggtga
     6481 tctcctgatg gatcagcgga tgctcaggag tcacttgatg aatcagctga atgagttttg
     6541 gctcttgaat gacttctgtg taccgctgtt caggttgata gcttaccacg tttaaatgta
     6601 gctgcatctt tggattaagc agtagtaaac gagaatcagc atcatcaatc aggaatacgc
     6661 caatcatcag ttcgttcagt aacgtatata attgctcttc tgtggtagta cacgcccgat
     6721 acatcttact catttgttcg ctcaacgcat taatcgtctg atacaattct tcccattgat
     6781 cgggtgtttg catgattact tccgcctgat taggattttg caccattttt tttagtacgg
     6841 gcagcacggt tttcaatggc tggttttttt gatagatcag ataatagatc attcctgcaa
     6901 gaacaaggaa gaaagacaga aagaagaaaa agacccaacg gcgaaaactc tcagttctcg
     6961 gtaaaaaacc gcttgtcggt tctgcgatac gtaaaatggc ttctaactgg ccactttttt
     7021 tgactggtaa tgcgacatat aaaagatcct cattcaaagt agtacttttg cgcaaagcgc
     7081 tacctaggtt cccaccattc aatacggctt tgatttcggg acgggagttt cgctgttcat
     7141 tcaatgtttc attgtttgtg tcaaataaga tctttccagt agcatccatt aaagtaatcc
     7201 gttcgttcga ctgatggacg aattcttcca aagcagtttt gttttgcgct gcttggaggt
     7261 tttcgacatc taattgccgg atcagcagtt gtgctttttt tgtcaggtag ctttcttgct
     7321 gttccacgat ctgctgttga aaataatggg aaatcaattg ccagccacca aaaaacaaac
     7381 taactaaaag aataagaaag aagagacgtt cataaatttt tttccatttc atcgttttgg
     7441 ttcctgaaac ttatagccaa atcctctaat agtcaccaag tactttggat gttttggatc
     7501 tcgctcgatt ttttctcgca aatgactaac gtgtacatct acgattcggc tttgtcctgc
     7561 gaagtcaaaa ttccaaatgc ggtctagcaa tgtgtctcgg tcgatcacac ggtcttttcg
     7621 tttcatgaaa taaacaagta attcaaattc tttcggtgtt agttcaatca gatggtcgtc
     7681 tacggtcact tgataattgg tcaaatcagc agtgatttgt ccaatcgata gatattcagg
     7741 ctcagcttca tcttttgatt gcttacgagg ttcaatccgt ctgaatattg ctttcatacg
     7801 ggctaatact tcgcgaggac taaatggttt ggtcaaatag tcgtcagcac ctatttctag
     7861 gccaatgatc cgatccactt ggtcatcttt agccgtcagc atcaaaatcg gtgtttcatt
     7921 tttttcttga cgcagccgct gagtgatctc catgccatcc atcgttggaa gcatcacatc
     7981 taaaatgatg aaatcaaatg attgttctaa tgctaattct aatccttcgc ctccatctgt
     8041 ggcactggtt accttgtagc cttctttttc caaattgaac gtcaagagcg ttacaataga
     8101 aggttcatca tcaacaacta gtattttttt cattcaaatc tcctttaaat aggtttcatt
     8161 ctgtttatat tttagcatag aatcagtaca aagggctatt ttacaaaaga aataaatggt
     8221 ataataggaa ggcactttgc tgaatagaaa ggaagagcag tcaatgattg gaatcagtgc
     8281 atgtttaggc ggtctggcct gtcgctatga tggacagaaa aaagaagtac cagaattgat
     8341 ggaactagtt gaaactggag aagctgtcat ggtctgtcca gaagtgatcg gcggattacc
     8401 aattcctaga gatcccgctg aaatcattgg tggaaatgga ttcgatgtgt ggaaagatca
     8461 agcaaaagta tggacgacaa gcggcgaaga tgtaacggaa gcgtacaaaa atggcgcgat
     8521 caaagcctat caaaaattgc aggaaaaaca cattgatcta gtcatcatga aggaaaaaag
     8581 cccttcatgc ggaacccatt cgatttatga cggaagtttt tccggaataa aaaaaaatgg
     8641 tgccggagtg gctactgctt attttattca gcaaaaaatg actgtgctct ctgatgaaga
     8701 atggctgaaa actagaggtg agcagaatgg aaatcgaaaa gaccaatcga atgaacgcgc
     8761 tctttgaatt ttattcgacc ttattgacag aaaaacaaat gaactacatg gaattatatt
     8821 atgcagatga tttttcatta ggtgagatcg cggaagaata cgaagtaagc cgacaagcag
     8881 tatatgacaa tatcaagcgg acaagcaaga tcttggagga ttatgaaaaa aaactccatc
     8941 ttttttctaa ttatattgtt cgagaacagg tattggaaaa tatgactgca tatattacag
     9001 ataagtaccc ggaagataaa aaactacttg aatatgtaca gcaaattcaa gcaatcgaag
     9061 aataaataaa aggaatgaag aaaatatggc atttgaaagc ttaactgaac gtctccagca
     9121 ggcgatggga aaaataagaa aaaaaggaaa agtatcagaa gctgacgtaa aagaaatgat
     9181 gcgggaaatc cgccttgcct tactagaagc cgatgttaac ttgcaagtag tcaaagattt
     9241 tactaagcgc gtacgcgaac gtgcggtagg ggcagaagtg ctggaaagtt tatcacctgc
     9301 acaacaaatc gtaaaaatcg ttgatgaaga attgacgatc acacttggtt ctgaaacagc
     9361 agagttaaat aaatcaccaa aaataccaac tgttatcatg atggctggtc ttcaaggggc
     9421 aggtaaaacg acctttactg gtaaattagc caattattta aagaaaaatg aaaatgctcg
     9481 tccgctccta atcgcagggg acgtatatcg accagctgcg atcgaccaat tgaaagtatt
     9541 aggtcaacag ctagacgttc ctgtatttga tatgggaact gaagtcagcc cggtagagat
     9601 cgtccgtcaa gggatggagt tagcaaaaga aaagaaaaac gactatgtat tgatcgatac
     9661 ggcaggacgt ctgcatatcg atgaaacatt gatggatgag ttgaaacaaa tcaaagaatt
     9721 gacgcaacca aatgagattt tgcttgttgt cgatgccatg actggtcaag atgccgtcaa
     9781 cgtagctgat agcttcaacc agcaattagg tattacaggg gtcgtcatca ctaaacttga
     9841 tggggatact cgaggcgggg cagcactatc gatccgctca gtgacaggcg caccaatcaa
     9901 attcatcggt tctggtgaaa aattgactga cttagaagta ttccacccag atcgtatggc
     9961 tagccgtatc cttggtatgg gggatatgct gacattgatt gaaaaagcgc agcaagacta
    10021 cgacgagaaa aaagcggaag aacttgcaca gaaaatgcgg gaaaattcat tcgatttcaa
    10081 tgactttatt gaacagcttg atcaagtgat gggtatgggc ccactggaag atctaatcaa
    10141 aatgatccca ggaatgaatc aagtccctgg aatcgaaaac gtcaaggtag atccaaaaga
    10201 tgttgaacgc aaaaaagcga tggtttattc tatgacgcca gcagaaagag caaatcctga
    10261 tctgttgaac ccaagccgtc gtcgccgaat tgctgccggt tctggaaaca gtgttgttga
    10321 agtgaatcga atgatcaaac aattcaagga atcaagaaaa atgatgcagc aaatgtccaa
    10381 aggggatatg aacatcccag gtatggacca aatgtttggt tcaggcgtca aaggaaaact
    10441 aggcaaaatg gcgatgaatc gaatgatcaa gaaaaataag aagaaaaaga aaaaacggaa
    10501 ataagatgaa aaaaacagct tcggtacaga ctgaaaatgt ctactgccgg agcttttcta
    10561 tcagcaattt ttattcttta ctaatacaat tactctaaac gaagaggtgg cacagatgag
    10621 atgtccgtgg agtgaacaaa cagaaagtat gcggcaatat catgatactg tctggggtgt
    10681 tcctgaatat gatgaccaaa agctgttccg aaaattgatg cttgatatta atcaagccgg
    10741 attgagctgg cagacgattt taaataaaag tgaagcattc gatgaagcat atgatcgatt
    10801 cgatatcaaa aaagtcgcaa attatggaga aacaaaaatc agtgaactaa tggaaaacaa
    10861 aggaattatt cgtaaccgaa gaaaaatcga agcagccgtc caaaacgcca aagcagtctt
    10921 gaaaattcaa gaagaaatcg gcagtttttc agagtatctt tggtcgtttt ctaatggaaa
    10981 aatcgttgat tcttcttatc attcacaaga agaagtccca acaacaagtc ctctttcaga
    11041 tgaattagca aaagatctaa agaaaagagg atttaaattt atcggaagca caacaatcta
    11101 tgctttttta gaagctgtcg ggatcatcaa tgatcacctt gacagttgtt tccgcaaaga
    11161 agaggtcgta cgtatcggca gaaagggcga ataaagcatg cgaaaaatca tattttatgg
    11221 tgctataagt ttagatggct atttggcaac gaaaacggat gatcttcaat ggctttttga
    11281 cactccaaca ggtaagcaaa ctacttatga agctttttat gaaacaatcg atacgaccat
    11341 catgggcaga aaaacttatc aggaagcaaa aaaatatttg gagaccgaag caatttatcc
    11401 agagaaaaag aatttcgttt tttccaccca cgcacaatta caattgccag atgctgaggt
    11461 cgtaaacgaa gatcctgttg cttttctaaa aaatctacaa atgacagaag gaaaagatat
    11521 ctgggttgtg ggtggcggtc gattgatcaa accgctattg gaacatcaaa tgattgatga
    11581 atggtatatc cagattaccc ctgtattatt aggggacggg attcctttat tccaaaaagg
    11641 aacatatgaa gcgcgttttg aaatgctaga cacgacacga ttcggtgaat ttatcgaatt
    11701 gcattatgta cgaaagtaga agtaaataaa ataaaaaagg caactatatg cagaaatgtc
    11761 ctgtacatag ttgccttttt tattaccgtt atgcttttta ctctgttaat ttgttagaaa
    11821 aatctagata cgcatctaaa atcgtttgga agaaacttaa tgttccttca acgatattac
    11881 cattttcatc aattagattc gcaacgtttc cgatatatgc ttccggttgc tgcaaggttg
    11941 gcacattcaa aaagacaagt gattggcgta gatggtggtt tgcgccaaag ccgccgatcc
    12001 cacctggtga tgcagtaacg actaaacctg gtttatgatc ccaaacactg tgtccataag
    12061 gtcttgagcc tacatctaaa gcatttttca atactgctgg cacagaacgg ttgtattcag
    12121 gagtaacgaa aacgacaccg tctagtcccc tcacttcctc acgaaaacga gtccattcag
    12181 caggagcctg ttctggagtt tcgagatcct cattgtaaaa tggcagatca tcgattttta
    12241 caaatacact ttcgtaccct tcaggaagca atttcgcaaa agcttctgct acttttttgt
    12301 tgtaagaatc ttttcttaaa ctaccaataa aaaatccaat tttttttgtc atacgatttt
    12361 tcctccttca aactatctat aataatctta caaaagataa gtaactggag caaataaaaa
    12421 cacttactga caaaagctac atagaagata gattacttat ttttaacaaa aaagaagtct
    12481 aaaatcttct attttctaaa aaaaatcgaa tttaatcaag ctgtaaagaa aaaaagcttt
    12541 acagggtaaa aaaaaactgt tacactacat cttgtgaata aaacaatgga ggtggaaata
    12601 aataatggca gttaaaatcc gtttaaaacg tatgggttct aaaaagagtc cgttttaccg
    12661 tatcgtcgta gctgattcac gttctcctcg tgatggacgt ttcatcgaaa ctgtaggtac
    12721 ttacaatcct ttgaaagacc ctgcagaagt agttttaaaa gaagacttag ttcttgactg
    12781 gttatcaaaa ggtgcacaac cttctgacac tgtacgtaac atcctttcaa aagaaggcgt
    12841 tatgcaaaaa caccacgaag ctaaattctc aaagaaataa ggtgaccgga tatgaaagat
    12901 ttaagcgact tagtcttaac aatcgttcgt ccgctagtca cttatcctga tcaagtcaag
    12961 ttagacgtcg tagagtcgaa agacttttac gagtacaatc taacagtggc tcctgaagat
    13021 attggccgta tcattggaaa acaagggcgt gttgctaaag caatccgcac aatcgtttac
    13081 ggtgttcgga tcaatgcacc gaaaaaagtg cgtttgaata tcatcgatga taaggaaaag
    13141 taaataaacg tgctgtactc gaattttcga gtgcagcttt tttgtttttt ttattaattg
    13201 aagaaatgat tatttggcaa aacaagcaag aaagcgtatc cttataggag gagggagtga
    13261 acagccaatg agaaagaaaa tgttatttat tccatttgct ctattgttaa gcgcttgtag
    13321 ttcggttact caagaatcag acaatacaca tctatcaagc agtatatctg aaactgttac
    13381 cagtccagaa aaaagtagta ccaaaacggc ttccaccact gaacaaacaa tggctacctc
    13441 aaataatgag aaaaagactg ctttagacca gttgaaagaa caacagccaa atgttcccat
    13501 gcctctagat gtaccagtct cttcaggcta tctcaatatc gcagcgacac atacaaagca
    13561 aggttattct attttatatt acagaacaga tcgtcccctc gggttgaatg ctgacgaatt
    13621 gaatcaagaa acgcctattg caacctatct atatcaatat gggtttgctt ctagtcaaga
    13681 aacaatccaa gttttgcagc catttgagat cgacacaaat ggtcagcaag tggacttagg
    13741 ttctcgaatc acagggtatc agcaaggtgc agcaggttca agttttcttg aatggcaaga
    13801 aggaaactgg cgtattcgaa tccgtgggaa taatatagaa ggccaagatc ccttactatt
    13861 agctaaagaa atcgtagctt atttggaaga aaacagtcta ccagcacctg aacaatttgg
    13921 taaaattact gttgatatgg gtgatacgac gaatcgagcc gtagaagtaa gctggcaaga
    13981 accgaaaaat gcctatacga tcactcatca agatcctatg tctgcgttga agatggcagt
    14041 ttctatgaag aggttgtgaa atcagcgcgc tgatctgagc ccatcattta actccaagtc
    14101 acaggaagta atcaaaaata gcacgccgca tttttgcata attttggctt aataccttaa
    14161 aagaaaacta gaattgcctc ttcacacatg ctaaaataaa acagacaaaa aagaaatggg
    14221 gatataagtt gactgaatac ttgaatgtag gaaaaatcgt aaacacacaa ggtatcaaag
    14281 gcgaagtccg tgttatctca acaacggatt tcccagaaga acgttacaaa aaaggaacgg
    14341 tgctaacact attccaagac ggaaaagcac ctgtggaatt aacagtcaaa agtcatcgca
    14401 aacataaaaa ttttgactta ttgagttttg aaggacatcc ttctatcaat gatgtggaaa
    14461 aatatcggga tggcatatta aaagtgtcaa aagacaactc tgtagatcta gctgaaaatg
    14521 aattctatta ccatgagatc atcggtctgc atgtattaga agatggaact gagttaggta
    14581 aagtaaaaga aatcttgtca ccaggtgcca atgatgtttg ggtcgttcaa cgtccgaaaa
    14641 aagctgatat attgcttcca tatatcgctt ctgtcgtcaa agaagtcgat ttagaagctg
    14701 gagtggtccg tgtagaaatt ccagaaggac tgattgacga tgaagattga tgtattgact
    14761 ttatttccaa gaatgtttga aggtcctatg ggggaatcca tcatcggtaa ggctgtagat
    14821 aaaggattat tagatatcaa tatctctaac ttccgcgatt attcagataa caaacatcag
    14881 acagtagatg attatccata cggcggtgga gcaggtatgc tgctcaaagt ccagcccatc
    14941 tatgataaca tccaagccat tgaaaaagct gcaccggatg tcaaaaaaag agtaattctt
    15001 cttgacccag ctggtaaacg attcgatcag aaaatggcag aagaattttc acaagaagaa
    15061 cacttagtat tcatttgtgg tcattatgag ggatatgatg aacgtatccg cacattagtg
    15121 acagatgaag tttccttagg agattatgtc cttactgggg gagaattagg tgcgatggtc
    15181 atgatcgatg caacagttcg gcttttgcca gatgtgttag ggaatcagac ttcagctcaa
    15241 acggattctt attcaaccgg tttattggaa catccccaat atactcgccc ggcagaatat
    15301 aaagggatga aagtaccaga agtattgaca aatggaaatc ataaattgat tgaagaatgg
    15361 caattaaaag aatcactaag aaggacctat caacgtcggc ctgatctttt ggaaacactt
    15421 gaatggacac ctcaaatgac gaaattttta gaagaaatca agaaagaaga acaagaaaaa
    15481 acagcagatg aagccaagtg actttttcta aaatcttctt tacagtaggt tgataatatg
    15541 ttagtatttt atggtgagtg caaaagcact taactattac gatattccgc tgtgagaaaa
    15601 gctcataaga atatttggaa taaggagaag aaaaccatga atccattaat cgaagaatta
    15661 acaaaagaac aattacgttc tgacatccca gcgttccgcc ctggtgacac tgtgcgtgtt
    15721 cacgcaaaag ttgttgaagg tactcgtgaa cgtatccagt tatttgaagg tgttgttatc
    15781 aaacgccgcg gtgctggaat cagcgaaact tatacagtac gtaaagtttc taacggagtg
    15841 ggcgttgaac gtacattccc attgcataca ccacgtgttg caaaaatcga agtagttcgc
    15901 tacggtaaag tacgtcgtgc aaaattgtac tacctacgtg cattacacgg aaaagctgct
    15961 cgtatcaaag aaatcagaag ataaacacgg aaaacttcct tggaaatctg aaatttgtga
    16021 aaagtccttg tatatcaagg gctttttctt ttttctctat cacagtttta agccattttt
    16081 ggctgttttg gaccattttt caatcatatt cccttaaaaa ttgtcctaga ttgtccgaca
    16141 ttgactatca gtgtcgtcaa aatagggcaa aatttagtag tcagcttgac gacaaatata
    16201 atacgaaaaa taccaaaaca tttaccgaat ttagcaactt ttgaacccaa gtttctcaaa
    16261 gggtcagcta ttagcaaaaa agacaaaaca tgtacttttc tactaaaaag ttgatgtgat
    16321 aggcttctat ctaaataact attaggagtt tttatcgtga agcaaaatga tggacgtatc
    16381 atttggaaaa atcaaagtga gttaaaactg attttaacaa ttaatgaatt catcgaaaaa
    16441 cacggaatta catctagccg tcaataccag aaaaagctat cagaaaatcc aaattctgca
    16501 ccaagtatgt ggttcattaa caaaaaatat ggatcatggg agaatttatt gattagtata
    16561 ggacgagaaa atactgggta tggaaaatgg gctagaatgt cagaacaaga attattggaa
    16621 atagtagaag cctttattaa gtgcgagaag ataacctctc aacgaatgta cgaacaaaaa
    16681 tcagtaggga agaatattcc ttcacttagt accattaaaa agatgttagg agatatacgt
    16741 ccacttttta aagaaaaaaa tgatggatct cgatttactg atttcgaact gctgttagaa
    16801 ctaaaaaatg aaataatccg attaaaacta caggatgatt tatcaatgac aaagtttcga
    16861 aaactagttc aatccccaaa actaccatca gttgatacaa ttatgaaaag aacgaacaaa
    16921 aattgggagg aattgatggc agagattgga tttgattatc gaagaatcaa aatttataag
    16981 cagagaaaca acctatccaa gacaaagaaa actaaatagt atcactctaa atcagacgtt
    17041 caaaattaaa atttggacgt cttttttatt acccaaaaac gaaaggagat ttattttatg
    17101 aaaaagccaa gatttaagaa ttggcgtttg ctcgcaacac tgagtctatt atgccaaaca
    17161 attggtggat cgcttggccc aacgattgcc tttgctgatg aaattactca tccacaaacg
    17221 gtaactgtgg aattggattt ggctcaccag tatgctgtcg aaggtacatt tagcgatggt
    17281 cgtcccatgt cagaagttac cgttccgcac tatgcagttt acaatggtgt gaagcaagat
    17341 attttctgta ttgagccagg ggtgccgatt tacaatgagt tcactcctgg ttatgagaaa
    17401 aatccattgc ctgatatgtc ggagaaagcg aaattagttt ccgttttatg gaaggaagct
    17461 ggtacggatg tggatacaca tattgtggcc cagaagatga tttggcaaga agttaatggc
    17521 tacacgcttc actcaattaa acgttctgat ggtagtgcag taaatatcgc agccattgag
    17581 tcaaaaatca atcaagccat tgcggattat caaaagaaac caagcttcca taactccaca
    17641 gtgaaaaccg tgttaggtca atcgactact gtgacagata cgaacggctt gaatttatcg
    17701 gagtttgatg aagttgtcga gaatatcgca aatattgatt atcgtgtgaa cggcaatcaa
    17761 ctagtgatta ctccaaatgc caattcaaaa gagagtggtg aattaaccct aaagaagtca
    17821 gctaatacag gaacaccggt agcttataaa atggctggtc aacaaacttt gatggcgggg
    17881 gcaattgata agcccaatac ctacacaatc aaaattgatg tggaaactga gggttctttg
    17941 aagattaaaa aagtcgataa agaaacagga aatgttgtac cgggaaccgt tttccattta
    18001 gactttggaa aaacattacc tgcaaaagac gtgaccaccg ataaagaagg cattgcgaca
    18061 ttggatgaga ttcctcatgg cgcgaaagta accattactg aaaaatcagt gccagctcct
    18121 tatacgattg acactacacc aatcaccgca accattaaat ctggtgagac tatttctgtg
    18181 acttcaaaaa atactcgcga aaaaggtcaa atcattttga ataaaatggg ggtggaaaca
    18241 ggaactgatc tttggaacga caaatactca ttagccggaa atacgtttgc cattcgcaaa
    18301 gacagtccaa ccggtgaaat tgtccaagaa atgaccacgg atgaaaaagg tcatgcggaa
    18361 acaccaaaag agattgccaa tgcgttggaa ttgggaacct attatgtgac cgaaaccaaa
    18421 gctagtaatg gctttgtgaa tacctttaaa ccagtaaaag tcgaattgaa ctatgtcaat
    18481 caaaccgtgg ctcttgttac cagtaacgta aaagggcaaa accaagaaat cactggtgaa
    18541 accactttga caaaagaaga caaagatacc ggtaatgaaa ctcaaggtaa agctgagttt
    18601 aaaggagctg aatatactct ctttactgca aaagatggtc aagcggtcaa atggagtgag
    18661 gcttttaaac cagagttagt gaagggaacg aaagcttctg atgaaacggt gactttgact
    18721 ttagatgaaa agaatcaagt tgctgttaaa cacttagcga ttaatgagta ttactggcaa
    18781 gaaaccaaag cacctgaagg atataccttg gatgaaacaa agtaccctgt ttccatcaaa
    18841 aaggtggatg ataacgaaaa aaatgccgtg attacccgag atgttacggc aaaagaacaa
    18901 gtcattcgct ttggctttga tttctttaaa tttgctggat cagctgctgg gactgccgaa
    18961 actggcttca atgatctgac ctttaaagta tcgccattgg aaggaaccag tgagattaca
    19021 gatgccgaag atgaagcaat tacagcttat aacgagcaac taggttttga tggctatggc
    19081 aagtttgaaa atcttcctta tggggattat ttacttgaag aagtagaggc tccagaagga
    19141 tttcaaaaaa ttacgccatt agaaatccgt tctactttta aagaaaataa agcggattac
    19201 actaagagtg aatacatttt tacgattacc gaaaaaggac aaaaacaacc aattaaaacg
    19261 gtgaccgttc cttacgagaa actgacgaat aaagcatttt ctgttagctt gaatcgtttg
    19321 atgctctatg atttacctga agaagaagat agcttaactt ctcttgggac ttggaaagac
    19381 ggaggcaaag aattgacaga ccttgattct accgagctag ttgataaatt gagctataac
    19441 ttgcatgaaa tcaaagaaga ctggtatgtg gtagcgcaag ccattgatgt ggaagcgaca
    19501 aaagctgccc aagaaaaaga tgaaaaagcc aaaccagtgg taattgccga aacaaccgca
    19561 acgttggcga ataaagagaa aacaggaact tggaaaattt tgcataaatt aaccgcagaa
    19621 caagttttgg ataaaaccat cgtcttattc aactatgtgt atgaaaacaa gaaaccctat
    19681 gaagcaggtg atgatccagt agcgaaagat gctagcttga acaatcaagc ccaaactgtc
    19741 aattgtacgg tggaacgcca tgtttccatc caaacaaaag cccacctaga agatggttcg
    19801 caaaccttta ctcatgggga tgtggtggat atgttcgatg atgtgtcgat tacccatgat
    19861 gtactggatg gctcaaaaga agcttttgaa acaattctgt atgcattact tccggataat
    19921 acgaccaaag aaatttggaa atctggcaaa attgattatg aagtgaatga caaagaattc
    19981 actaaaaccg tacttgcgga aaaagtggac acaagtaaat atccagaagg tacaagtttt
    20041 accttcaaag aaattaacta tggcaaagac ggaaacatca atgggaaaca caatgaggat
    20101 ctaaaagaaa aaactcaaac tttaacacct aaaaaagttc cgacaacacc aagtgcacca
    20161 gaacaaccgg aaataccaac tgttccaagt gactctcaag aatctagtcc tacagtaaag
    20221 acattcccac aaacagggga gaaaaattct aatgttctgc tgtttattgg gtttaccttg
    20281 atctttgcga ctgctgggta ttatttctgg aatcgccgaa actaaggtga tgtgatgaaa
    20341 caactaaata ggaaaaacca acgtgcgcga ccgcctccta aaaaaagtgg tcgcgctatt
    20401 tttgtatcca aaactaaaaa tgaaaagagg aaaagaagat ggaattgaaa tttgttgtgc
    20461 cagatatggc cgaaaccttt ggaaagataa ggtatgccgg cgaaggcgaa gttttaactg
    20521 aaggatatgg gcggaatact acagtaattg gtcgtagtta tcatttgtat tccagtaagc
    20581 aacgagccga tgatattgaa gtggtggtag ctgcggaagc cggcgaaaag gattttgatc
    20641 aagatcaacc actaaaagcc gtgaatcccc atttggttgc caaaggctat gagattgaga
    20701 atcgtgggtt taccgactac gtgttgtatg tcgatgattt agtgaaagca taggaggaaa
    20761 agaagatgag attaacagaa ggtattgttg tagattcagg actaacgttt gggaaattac
    20821 gattttccgc attacgccga gaagtacgga agcaaaatga ggatggaacg gttagtaacg
    20881 aggtaaaaga acggacttat aatttgaaat cctctgcgca aggtcgaatg atccaagtta
    20941 gtatcccagc aaatgtaccc ttacgcgagt ttgcctatga cgcagaggta gagttggtca
    21001 atccaatcat ggatacggtc gccaattatg ttttccgaga aggaactact gtcaattggt
    21061 tcattaaggc cgatgattta gtcttgaaac gacaaccaga tcagggaaat tctacaaacc
    21121 aaaatgaagg aaagaaatag gtgaagcgta tggaaattaa tgttgaacat attttagatt
    21181 gtctcgatca atatggaaaa ggtgagctaa cggaggaaca actcactaaa gcattaacct
    21241 atgatgaaaa aatgtttctg attatgcatc aagggttact ggaagtaggt aacgatgccg
    21301 aagaagattt tgatgtactg aattggttag gggaagaaga gtccttcttt atggttatcg
    21361 aagtaaatga aagtctatgt cgagaagccg aaaccgtatt ggaagaaatt ggtgtggaaa
    21421 tgccggatgc cattgaagtt tttttaaagc aactagtgga aacgaaacaa ctccctatag
    21481 cagtaaatga ctagcataac tgcttgaaat tggatcaatg aaatcctatg aagctttttt
    21541 cgattggagg tggaaaccat aaaaatgtac aaagggcatc gtatacgggc aggagatcag
    21601 cacttggttt atcactttgt tcttggctgg ctactcgctc tgttcatcgg ttggatgagc
    21661 gtcttttatt ttcaagagct tagacaattt gatatttcta agctatcact ttctactata
    21721 gaaatcgttc gttctataaa agatttaatc tatttgctag gaagtctcgt tctttcagga
    21781 agcacgatgt tgctttatat acactttttt caggatcatt ggcgcagttt gtggcatcga
    21841 caaaagctag cccggatgat tctagaaaat cattggtacg aagtaaaaca aactcagagt
    21901 gaaggatttt tcaaagacct caatagtagt cgaaccaaag agacaatcag ttacttcccc
    21961 aaaatctatt accgtatgaa ggatggttta ctttctattc gcgttcaaat ttcactggga
    22021 aaatatcaag atcagctttt aaagctggaa aagaagttag aaagtggtct atattgtgaa
    22081 ctagtagaaa aggaactcaa agactcctat gtggagtaca ctttactgta tgatataatt
    22141 gccaatcgaa ttgggataga cgaagtagtg gcagaaagtg gggctttgcg attgatgaaa
    22201 aatcaagtat gggcttatga ttctttacct catatgttaa tcgctggcgg cacaggtggt
    22261 gggaagactt actttttact cactatcatc gaggctttat taaaatctga tgccgagctt
    22321 tttgttcttg atcccaaaaa tgcggactta gccgatttag gtacggtaat gcctcatgtc
    22381 tattcccaaa aggaggaaat ttctgcttgt gtggaagatt tctatgagcg catgatgact
    22441 cgtagcaagg caatgaaaga aatgcccaac tacaaaacag gagagaatta tgcgtttctt
    22501 ggacttccac caaacttttt aatctttgat gaatacgtgg tttatatgga aatgttaacg
    22561 acgaaggaaa gtgcggtgat tttgaataag ctcaaacaaa tcgtgatgtt aggtcgtcaa
    22621 tctggtttct ttctgattct agcttgtcaa agaccggatg cgaaatattt aggagacggg
    22681 attcgcgatc aatttaattt ctgggtggct ttgggtcgta tgagtgaact gggatattcc
    22741 atgatgtttg gcgaggttga taaaaaattc tttatgaagc gtatcaaagg tcgaggctat
    22801 gtagatacgg gagggagtgt gattagtgag ttttatacgc cacttgtacc aaaaggatat
    22861 gatttcttaa gggaaattag cgacattggt ggattaaacg ttaatacgga aggataaaat
    22921 aatagtatgt agcctaggag tgtcaagaat cctgggcttt ttttacgagg tgatttatga
    22981 catttttaga tttattcgct gggattggtg gcttttgctt aggtatggaa caagcgggtc
    23041 accagtgtat cggcttttgt gaaattgatg attttgctcg ccagagttat aaggcaatcc
    23101 atgatacaag taaggaggtg gaaatgcatg acatcacaag tgtatcagac gagtttattc
    23161 aatctctcgg accagtggat atcctttgcg gtggatttcc gtgccaagct ttttcaattg
    23221 cgggaaagcg gcaaggattt tccgatactc gaggtacttt attctttgaa atcgctcggt
    23281 tcgccgctat tctccaacct aagtttttat tccttgagaa cgtccgggga ttactcaatc
    23341 acgaaggagg ggctacgttc gagacgatcc tccgaacgct ggatggattg gggtatgatg
    23401 tggaatggca agtgcttaac tcaaaggcct acgttcccca aaaccgtgag cggatcttcc
    23461 ttatcggaca ttctcgagac gcatgtaccg aacaagtatt tcctatcatt ggatcgtctc
    23521 caacatctga tcaaaacatc agaaacttat taaatatcaa cccttccaat cgaggaatgg
    23581 ggggacaagt ctatgggtca gatgacgtag cgcctacctt gaccggtgat gaaggaatca
    23641 aaatcgcatt gccggtagat gatggaattt ctgtagcggg tatgttactg ggaaattttg
    23701 aacaaggcaa tcgggtttat gagataactg gaacagcgcc tactttgtca acgaaacaag
    23761 gcggaacgaa gatcatgctt cggaaaaacg aaacagaata tcaagaggtg aagccaggga
    23821 acagtgtgaa cctggctttt cctaattcca caagtcgtcg aggccgtctg ggaaaacaaa
    23881 gtgtgcatac cttattaaca ggagaccaac aagcagtcgt cacggatcag tatcaaattc
    23941 gaaagttaac acctagggag tgctggcggc ttcagggctt tccggactgg gcttttgatc
    24001 gagcttctca agtcaattca gatagtcagt tgtataagca ggctggaaat tctgtaacag
    24061 ttccggtcat ttttgatatt gctagaagat taaaggaggt aaaaacagat gatttatgat
    24121 gagttgattg gggaatctac tgggtgattg gcaagattca gtcagatccg gaattggaag
    24181 aagaactgta ccggctcaat tttgatattc gcaaaaacgg ggtgaaagtc cctgatgatc
    24241 cgtatgtaat tgatgaggaa acggatgctc gaatggagct gaatcaagtc atcagtgaat
    24301 tggaacgaat tgcggacttt gctaaagaac ctgatatccg ccaatacttg tttgagataa
    24361 aagcagagtt ggaaattgag ggtattaccg cggagtaacg gccaaataaa ccactgatag
    24421 gcactgaccg cgggggattt gcgaagcaga aatcctccgt ggtgctgtgc ctgtttatgg
    24481 acggcgttag ccggccatga tgtctcaccc cccgtatcta acaggggggt acaaaaacac
    24541 aaaaaataag caagcaacga taaggaatgg ctgtcctatc aaggataata agcaatttgt
    24601 gtagatgtta actgatttga aaaagttaac accttaaatt tggaggtgag aacttggcac
    24661 aaaggaattt agattatcgg ctattaaaag accggcgtaa tgaatatggc gtttcacaaa
    24721 ataaattagc tacgacttgt ggacttagtc gcccgtactt aaatcagata gaaaatggtg
    24781 gcgtaaccgc atcgacaaaa accatgagga aaatttttga ccaactggaa agtttcaatc
    24841 ccgagttacc tttaacgtta ctgtttgact atgtaaggat tcgctttccc acaacggatg
    24901 cgaggaaaat cattcaagag attctccatt tgaaatttga ttatatgctc cacgaagatt
    24961 acgcctttta ctcctatcaa gaacaatatg ttatgggaga tattgtggtg atgttgtcac
    25021 atgaagaaga taaaggcgtc cttttagaat taaagggtcg tggttgtcgg cagtttgaaa
    25081 cttttttact cgcccaaaag cgtagctggt atgacttttt cgaagattgt ctaaaagccg
    25141 gtggcgtgat gaaacgctta gacctggcaa tcaatgatcg agttggcctg ttagatattc
    25201 ctgatttaac gaaaaaatgt cagaaggaag aatgtatctc cttgtttcgt accttcaaaa
    25261 gctatcgttc cggggaactg ttgaaacctg atgataaaga tggtatgggc aatactttgt
    25321 atattggcag tcttaagagt gaggtctatt tctgtttgta cgaaaaagac tatgagcaat
    25381 atatcaagtt agggattcct ttagaccaga ccgaaacgaa aaatcgtttt gaaatccgac
    25441 tgaaaaatga tcgagcttat catgcaattc aagatttgtt aaaaggtcgc agtattgaaa
    25501 gtaccacatt ttccattatt aaccggtact tgcgatttgc ggataaggtg gaaggcaaaa
    25561 ggcgaactaa ctggcccttg aatgaacaat gggggcgctt cattggacgg aatcagaagg
    25621 aaattcaatt aacttctgaa ccgaagcctt ataccattga acgaacgttg aactggcttg
    25681 gacgacaagt cgcccctacg tggaagatgg ccaaggagtt ggatcggttg aatcagacaa
    25741 cgtacattca ggatatggta aataatactc gactatcaga ccggcacaaa aagattttgg
    25801 agcaacaaag tatggcaatt gaaaatttga ttatatgaaa agaggaatcg tgatggaaat
    25861 tgtgaaagat attttattgg tgtttggtgg cagtatgctt ggtgttactg tgatgtgctt
    25921 gatgcatgca agtgcagcgg cggaccgagc aatggaaaag aaaggaaagt gatgaaatga
    25981 atttcggtca aaatttgtat caatggtttt taaccaatgc tcaatcttta gtcttactag
    26041 cgattgtggt cattggtttg tttttaggat ttaaacgcga gttttccaaa ctgattggct
    26101 tcttggtgat tgctttgatt gcggtaggtc tggtctttaa tgcttctggt gtgaaggatg
    26161 tcttactcaa tttgtttaac cgaattgtcg gtgcctgagg gggaatcaca atggaacaga
    26221 tacgtgttta tattgcgaac cttggaaaat acaatgaagg tgagttggtg ggggcatggt
    26281 ttaccccacc cgtcgatttt gatgaagtga aagaacggat tggattaaat gacgaatatg
    26341 aagagtacgc aattcatgat tatgaactgc cttttgagat tgatgagtat acaccaattg
    26401 aggaaattaa tcggttgtgt ggtttagcgg aagagcttaa aggaacgcca attggtgagg
    26461 tcgcttcaga gattcaacat gccttcttca attctttcga ggaaatggta gagcatgtgg
    26521 atgatatcat ctattatccc gattgtaatg acatgagtga tgtagcgtat tacttgattg
    26581 tagaagctgg tgatttaggc gaagtaccag aacacttgaa gacgtatatt gattttgatg
    26641 cgtgtggtcg agacttggag attggcggga attatttggt tacgagtcga ggggtttttg
    26701 aactaatgta aatcatttgt tagaattgac cagaaatttc tatcgtactt ttacttttct
    26761 atttgatata ctgaaataga aaatatttgt tgataggggt gttactaatg gttggcaata
    26821 agtggtacaa atgtgatctt catattcatt caactgcgag tcagtgcttt caggataaaa
    26881 aggttactgc taaggaatgg gtagaagaat gtttaaacaa aggactcgat tgtgttgcat
    26941 taactgatca caatacaggg gaaaatattg atgaatataa gaaagtggct atcgaaaatg
    27001 gcttagattt ttttcctgga gtggaattaa cgtgtggaga aaatggaact catttactag
    27061 tcctatttga taaaagtgca tgtacaaatg atatctctga tttcttaacc aagatgggta
    27121 taaaaagaga ggattgggcc agcgctgcag cttgctcaat gaaatcagtt ttagaagtta
    27181 ttgattttgc tgagcacgag ggcttgctcg ttattcctgc acatatagat gagtttaatg
    27241 gaatttcttc agcaaaattt ggaaatctag gaaagatatt tgaatcagaa aatatatatg
    27301 cagtacagac ggttcaaaaa gaattttttg aaaatcgctc acaaatgatt ccctcatcta
    27361 agcgtgacac tatctatgat agtgtgaatg ccagatatga tggtagagtt ggaaaagata
    27421 ctttggaatc ttggtataaa tcagttgttg aggcagaaaa aaataatatg acatttctta
    27481 gcttttctga taacccacac tcgaaaggaa attcaaaaca tggactttgg ggaataggca
    27541 caagatattc ctatataaaa atgaaggacg aacctgactt gggatcactt cgcgatgctt
    27601 taatgctagg cgaaactcga gttcattctg atttttctaa tttctccatt aatgaaaatg
    27661 aagttttatt ggaaaaatta agcttttctg ggaccactct tagcacaaaa gaagttgtag
    27721 tcgaattcag cgataattta acttcaatta ttggaggaag aggaacaggc aaatcttgta
    27781 ttacaagatt cttggtctat gtccttggaa aggaagcaga acttgatcaa ttttctgaaa
    27841 tacaaagtga ttatcagaac tttgcacaaa ttgaacataa tggctctgga atatttttga
    27901 aggatacaat tgtgaaactg aatattttct ataaggggac aaaatatcaa ataataagaa
    27961 cacaggatag acattctatt tatgaattta cacaagagaa tggtctggtt gaaaaagaaa
    28021 cagaacggtt gagaatgatt tccgataagg tgagtatata ctctcaaaaa caaatttatg
    28081 aaatttcaaa aaatcaaact agtatactag aattactaga tggatataac tctgatttga
    28141 tttcagagta taaaaatgag attgaaacat gtgtaaacga aataagaaaa ttaaattggg
    28201 atattgtttc tgtgaaaaaa gaaatacaag ataaggcgaa ggtagagctt gaaattgaag
    28261 atttaaaaaa acaagtagaa aagctatctc ataagagtta taaagatgtt tatgatgagt
    28321 attcaaaaga gacagatatt tatagggaac tgaaacgtga tgtggaggca cttaaggaaa
    28381 ttcccaaaaa tctaactgat agtgttgaca aaattgactt tggcaatggt ataaaagtta
    28441 cagacgaaat tgatgaacac cgcggagaat tgattaagaa tttagtgacc aatcgggcta
    28501 agattcaaac aataattaat gaaatgtcag atgaaattga tagttataga gcagagctga
    28561 ataaatcaga ttggaaagtt aactataatg aggtttcaaa gaggtatcaa agaggtatca
    28621 aaaacttagt gaagagttat ctcaagatga attaaaaatg ttacaaaacc ttgattcttt
    28681 atatcaatct ttatcaaaaa ctgaagagcg attggaagaa attattcaac aagaaataaa
    28741 gcttaaagat taccaagttg aggttgactc agcaatgtca agaatgaata aatcatatca
    28801 aaatttctat tctgagagaa gtagttcctc gcgcgaaatt ttttctgata ttacaggaat
    28861 agaggctact attcaaccaa atagggattt caataattat gttaaagagc tcagagcttg
    28921 tattaataag gaggacactt ttgacagtga gtttggatct cttattgata agctgacaaa
    28981 aaattccatt
//
LOCUS       DAZKLM010000027        28457 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3764
            SAMEA115706913-rid22582563.denovo.027, whole genome shotgun
            sequence.
ACCESSION   DAZKLM010000027 DAZKLM010000000
VERSION     DAZKLM010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706913
            Sequence Read Archive: ERR13196647
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 28457)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28457)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287823.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287823.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,004
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,849
            CDSs (with protein)               :: 2,849
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 41 of 96
            Pseudo Genes (incomplete)         :: 52 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 15 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28457
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706913-rid22582563.denovo.027"
                     /isolate="VRE3764"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            91..1731
                     /locus_tag="ACG842_001102"
     CDS             91..1731
                     /locus_tag="ACG842_001102"
                     /EC_number="4.1.1.101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043883 - malolactic enzyme activity
                     [Evidence IEA];
                     GO_process: GO:0043464 - malolactic fermentation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malolactic enzyme"
                     /protein_id="HFQ0247662.1"
                     /translation="MLTGLNLLNNPFLNKGTAFTKEERAKYGITGMLPSTVQTLEQQS
                     VQAYGQYLSKQTDLEKRIFLMNLFNTNRTLFYKLMGEHLVEFMPVVYDPVVADSIEQY
                     NEIFLKPQDAVFLSIDEPENIKASLKNAADGRDIRLIVVTDAEGILGMGDWGVNGVDI
                     AIGKLMVYTAAAGINPAQVLPVSIDAGTNNEELLNNPLYLGNRHARVEGETYYEFIDQ
                     FVQSATELFPELLLHWEDFGRGNAATILEKYEDKITTFNDDIQGTGIVVLAGVLGGLN
                     ISGEQLKDQTILTFGAGTAGVGIANILLDEMIRQGVPEEEARQHFYQVDKQGLLFEDT
                     KGLTPGQIPFARKRSEFTNADELTNLEAVVKAIHPTIMIGTSTQPGAFTEEIVKEMAA
                     HTPRPIIMPLSNPTKLAEAKAKDLIEWTDGKALVGTGIPAADVEYNGVTYQIGQANNA
                     LMYPGLGLGLIASTATRVNAEIISQASRALGGIVDVTKPGAAILPPVAKITEFSQTIA
                     ETVAKSVVAQKLNREEITDIKEAVESAKWVPEYKSLED"
     gene            1885..3207
                     /locus_tag="ACG842_001103"
     CDS             1885..3207
                     /locus_tag="ACG842_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxycarboxylate transporter family protein"
                     /protein_id="HFQ0247663.1"
                     /translation="MAQKTTTATAVEEKKKEGFWSTKITGVSLPLYLIMVLVLIIAMA
                     LGKLPTNMIGPIAVLVIFGNLFHFIGTKIPIVKSYLGGGSVFAIFASAAIATFGILPE
                     YVVKSTENFVSNMGFLDFYIAALITGSILGMNRNLLMKASVRFIPVALISMVVSFFAV
                     GLVGMLIGNGFANSVLYISLPAMAGGVGAGAVPLSTIYANVLGTDASSIISRLIPATA
                     MTNVLAIIGAALVARAGQSMPKFNGNGKLMEKGDLGDGKPRNVKPSIPQLGVGLMVSL
                     TFFILGQICNYFVPKVHAYAFMIIIVVICKIANLLPEYYEDAAIMFNNLIVKNLTAAV
                     LAGIGIALLNLNVLASALTWQFVVLCLTSVIAISVVSGFVGRLFGLYPIESTITAGLC
                     NNSMGGTGNVAVLSAANRMELIAFAQMGNRLGGAIVLIISGFLMQLLS"
     gene            complement(3269..3700)
                     /locus_tag="ACG842_001104"
     CDS             complement(3269..3700)
                     /locus_tag="ACG842_001104"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0247664.1"
                     /translation="MLREMRLGDAEALREINAEQLGYDVPLTVTVRQMKKLLKEPEKN
                     FFLVYEDPTSKKAAGYVQAESYESIFSDPMFNIMALAVSKHAEKRGIGKALMTGVEEA
                     KRRGISAIRLNSAEYRVEAHRFYERIGYHSDKMQKRFLKIL"
     gene            complement(3764..4600)
                     /locus_tag="ACG842_001105"
     CDS             complement(3764..4600)
                     /locus_tag="ACG842_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HFQ0247665.1"
                     /translation="MIGLIYFVVIVLANTVGAISGMGGGVLIKPILDLIGAHSVAGIS
                     FYSTVAVFTMSIVSTVRQVSSGKSLNWQIVGWVSSGAVVGGVVGNIVFEVFLQLFENE
                     KHVQLIQIFLTVLTLVFAFFYTKHHQPKFHLTSWTWYLICGGVLGFLASFLGIGGGPV
                     NVSLLMLMFALPIKEATLYSLSTIFFSQLAKLVTIALTSSFMRFDLSMLFYVIPAAVI
                     GGLWGARFSRVLSPKKVTFIFQAIVIVVLLINLYNAFVILQTLEGSGFKQVKITEKSF
                     FK"
     gene            complement(4790..5092)
                     /locus_tag="ACG842_001106"
     CDS             complement(4790..5092)
                     /locus_tag="ACG842_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HFQ0247666.1"
                     /translation="MTEHNHSHEHEGHEHITLVDDQGNETLYEILLTVDGQEEFGRNY
                     VLLYPAGVSEDEDVELQAYAYIENEDGTEGELEQIETDAEWDMIEEVFNTFMAEEE"
     gene            complement(5118..5549)
                     /gene="ruvX"
                     /locus_tag="ACG842_001107"
     CDS             complement(5118..5549)
                     /gene="ruvX"
                     /locus_tag="ACG842_001107"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HFQ0247667.1"
                     /translation="MRVMGLDVGSKTVGVAVSDPFGWTAQGVEIIRINEAEEEFGFDR
                     LKELVAQYEVDRFVVGLPKNMNNSIGPRAEASMSYGEKIKELFSLPVDYQDERLTTVQ
                     AERMLVEQANTSRAKRKKVIDKLAAVMILQNYLDGSGKQLF"
     gene            complement(5546..5812)
                     /locus_tag="ACG842_001108"
     CDS             complement(5546..5812)
                     /locus_tag="ACG842_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HFQ0247668.1"
                     /translation="MGFTDETVRFGFDDSRKKEVSETLAIVYRALEEKGYNPINQIVG
                     YLLSGDPAYIPRYRDARNLIRRHERDEIMEVIVKDYLSNHGVNL"
     gene            complement(6066..6791)
                     /locus_tag="ACG842_001109"
     CDS             complement(6066..6791)
                     /locus_tag="ACG842_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HFQ0247669.1"
                     /translation="MSKKTTKAIVGIVAAVIAGALGISTTQKDSDIFQQITTIFDSTP
                     QASQVADVPEYDGKHQEIEINGNQPEFTKEDLSLSKGTWESYSNIDKLNRVGQANAML
                     GKDLFPTEKREPLYIDPTGWKQKKLSDGQWLYNRSHLIGFQLTGQNNNIKNLMTGTRS
                     LNAPYMLAHENDIAAYIKETNHHVRYRVTPHFEGNELVARGVQLEAESIEDKKIEFNV
                     FIYNVQDGYTINYETGQATKTKN"
     gene            complement(7157..7227)
                     /locus_tag="ACG842_001110"
     tRNA            complement(7157..7227)
                     /locus_tag="ACG842_001110"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7193..7195),aa:Gly,seq:ccc)
     gene            7344..8096
                     /locus_tag="ACG842_001111"
     CDS             7344..8096
                     /locus_tag="ACG842_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HFQ0247670.1"
                     /translation="MTGKELDLLLDTCLLAGKIMMESNAEMYRVEDTMSRIALASGNF
                     RLVSYVTQTGLFIGLDRTSTIRMEQITNRSINLEKVVKVNDLSRKYVASELTLEELYC
                     ELQAIDRDRSFFPVWLQIVSAAVISGTIMVLFGGKMTDLPVTLVIGGLGYAAYLYSLK
                     FFRIKFLSEFLSSLLIGIAAIYSVRFGLGSNQDLIIIGCVMPLVPGVQITNAIRDLLA
                     GHYVSGVSRGTEAMMTATMIGFAIAFIFQLFY"
     gene            8112..8591
                     /locus_tag="ACG842_001112"
     CDS             8112..8591
                     /locus_tag="ACG842_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HFQ0247671.1"
                     /translation="MGTIFIQFSFSFLATAAFAVITNVPRRSLICCGLSGAFGWMIYW
                     ICVQIGGSAAFGSLLGSLGVAFISDLFSKRLKMPVTIFNIPGMVPLVPGGLAYQAVRN
                     LVTESYREAAHYAVQAIMIAGAIALGLVLSEVFNHNIRNFKVKRDAIMMKRKKRLFK"
     gene            complement(8842..10422)
                     /locus_tag="ACG842_001113"
     CDS             complement(8842..10422)
                     /locus_tag="ACG842_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HFQ0247672.1"
                     /translation="MYKRQLLELIRVNLLYVNPQLTNKAREKGKKGKRLTIYLLSQNL
                     FSGLIFLLIYGFAMFAMNFVKLPGFFTYYVALFGMLGLTQSISVIYNIFFEGNDLQVF
                     LPLPFGQEQVFSAKILVVALTIIPFVFPLLVMFILTGWRADLGILLTIIISILLFSLF
                     LIILFCLASLIVFGLARTAVFKKHRKTVSSILLGSSMLIAFAGIMWMNHQTGTIENEL
                     TDRHPISILLPFYDAASQPLSQQGLLGWGILAITALGFFLAVKQLIVPKLTEQLTDSN
                     SEFKIKRTHKKNQNVHQLLFSYNAQLVKEPNLIMQVLSSSLLTPIIFILAFALGGEIK
                     LTDLDNKLIGVVFLVGIALAFLTVNQTSFISNLISLDQENFLFIRSLPISMNQYLKEK
                     FRFGWILQSFLTGGIALLSGLSFQLPLFFIPSLLFGSLFGCYLLSLRYFARDYRLLLL
                     NWTNINQLFNRGSGSLGLVATMMGSLIISIILIVLYGMLALFYPFWLVNIPVIGLVLI
                     LSALWIVYYQRTFWKKFE"
     gene            complement(10415..11158)
                     /locus_tag="ACG842_001114"
     CDS             complement(10415..11158)
                     /locus_tag="ACG842_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0247673.1"
                     /translation="MIEFINVSKSYGKKQALKDLSLSIRQGEIFGFLGHNGAGKSTTI
                     KSLVSIIEPSSGTILVDGMKLTENRLSIKQKIGYVPDSPDIFLQLTAGEYWDLISAAY
                     ELDTQKKEKRLAELTALFDMYSHQNETIASFSHGMRQKTILIGTLLPDPDIWVLDEPL
                     QGLDPQAAFDLKEMMKAHAAKGKTVIFSTHVLDTAQQLCDKLAILKKGELIYQGAVSE
                     LLNGSPDETLEKIYLKMAGRQATGDGPYV"
     gene            complement(11334..12110)
                     /locus_tag="ACG842_001115"
     CDS             complement(11334..12110)
                     /locus_tag="ACG842_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytR/AlgR family response regulator
                     transcription factor"
                     /protein_id="HFQ0247674.1"
                     /translation="MVTRIFILDDSLVFEKMLVEILEESRILLSPWKFEIVQGLNVIQ
                     FVEFIKNNEIYSSDIFFLDIDLNAYYSGIELGEMLREKNEKCKIVYLTSHPNKAITVI
                     NRKITPSGYLIKEFTYEKNKKSIQSYLLKEKENYPVQHATKKRWTTVRYGNSDHYIHY
                     DEILYLMSMPGYKNKIGLVLKDEEILVNGTIKKYKKSLEYEYLCKDLKAYIINTENIT
                     SISRVNEIVTFANGEQLEMGKRSIDKLKNFMQEKIENESF"
     gene            complement(12358..13194)
                     /locus_tag="ACG842_001116"
     CDS             complement(12358..13194)
                     /locus_tag="ACG842_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HFQ0247675.1"
                     /translation="MLRFLILSGYFELMMYLQVSGKLDQYINVHYRYLAILSMILSAL
                     LALVQLYLWVKNGKAEEKAHEHDHDHGLSKPYQRGIAYVLLALPVIVGTLFPTVSLDT
                     TIVEAKGFNFPVSKESVGDPEMQTQYLKPDTSIYFNKSDYLDQMNELKEKYGDKQTIK
                     ITDENYLEVMELIYNYPSEFIGKKISYEGFVYQTPNDSNTDIFLFRFGIIHCVADSGV
                     FGLLTHMPEGVTVKNDEWYKIEGTIQSDYYQPFKREIPSVVVTNAEKVSAPKNQYVYR
                     SF"
     gene            complement(13205..14134)
                     /locus_tag="ACG842_001117"
     CDS             complement(13205..14134)
                     /locus_tag="ACG842_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HFQ0247676.1"
                     /translation="MFSFIPNSVLQMSTIFLSIVIEALPFVLLGCLISGALQVFLTPE
                     RVKRWLPENRIFSIFTGSILGFFFPSCECGIVPIVHQFVKKGVPVHTAFAFMLTAPII
                     NPIVIFSTFIAFGNSWQMASWRVIGSFFVALVIGVWLAYFQKDAVLKEKITSKVAENH
                     HHHVHEEEQSEKNEPKNQLIHQTGHVLTHSIDEFFDTGRYLIIGGLIAAAMQTYLPTR
                     IMLTLGSTKILAIIIMLLLAFTMSLCSEADAFIGSSLLSLFGTAPVAAFLVFGPMVDI
                     KNLLMMKRYFTGRFILSLIGLIALSVIGFTLFI"
     gene            14326..14679
                     /locus_tag="ACG842_001118"
     CDS             14326..14679
                     /locus_tag="ACG842_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HFQ0247677.1"
                     /translation="MKQTTNLTEVQDILQQSSVAVIYLSMPNCSVCHAVRPRLEELLT
                     HYDIPALHLDAFETPEVASKFEVLTAPVVLIFHQGKEVFRQARFIDFKKIRYLLDELT
                     AEDDSLSYEEIFRTK"
     gene            complement(14753..15409)
                     /locus_tag="ACG842_001119"
     CDS             complement(14753..15409)
                     /locus_tag="ACG842_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0051775 - response to redox state [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HFQ0247678.1"
                     /translation="MKDHIIPKATAKRLPLYYRYLRILNNAGKKKVSSTELSEAVQVD
                     SATIRRDFSYFGELGKRGYGYDVEDLMHFFAKTLNDDQLTNVALIGVGNLGSALLKYK
                     FHQSNSIRISCAFDVNEEIVGRIVDGIPVYPMEDMSEQIKIQQIEVAILTIPAERAQE
                     VVNQLTEAGIKGILNFTAARLNAPSDVLIQNVDLTNELQTLIYFLHSDANAHSENTAE
                     "
     gene            15635..17569
                     /locus_tag="ACG842_001120"
     CDS             15635..17569
                     /locus_tag="ACG842_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HFQ0247679.1"
                     /translation="MILLQANQIARHFGAEVLFENIHLEISTGARVALVGRNGAGKST
                     LLKIIAGIEAPDEGKIAKNKTATLGYLAQDTGLDSNETVWNEMLKAFEQVRQMEERMR
                     ELEIFISEGTPDTPAYSSYLKEYDRLQHDFSDRNGYGYENEIRSVLHGFKFDSSFYDQ
                     KIDTLSGGQRTRLALARMLLQHPDILILDEPTNHLDIETLSWLENYLQSYTGALLIVS
                     HDRYFLDKVVNEVYELSRRKMRHYKGNYSKYLDLKAEQLASEWKAYEKQQTEIHKLED
                     FVARNLVRASTTKRAQSRRKTLEKMDRLDRPQGDEKQANFLFSIEKPSGNVVLQIEDA
                     AIGYDNVTLSEPINLDIRRQEAIALVGPNGIGKSTLLKSIIHQIPFIRGKETLGTNVT
                     IGYYDQGQADLHTNKTILAELWDEHPTTPEKEIRSALGAFLFSGEDVEKPIPLLSGGE
                     KARVALAKLAMNKENFLILDEPTNHLDIDNKEVLENALIDYDGTLLFVSHDRYFINRI
                     ATKVVELSENGSKVYLGDYDYYLEKKQEEEEIAELLAQDEPESAPAPTNKSDYYQSKE
                     QQKLIRSLQRKITQVEEEMARIDEWIDQLEQEMVAPENLEDHVKLNELNQDLEAARQD
                     QETKLTEWEELSLELEEYEN"
     gene            complement(17655..18878)
                     /locus_tag="ACG842_001121"
     CDS             complement(17655..18878)
                     /locus_tag="ACG842_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HFQ0247680.1"
                     /translation="MLKAGGEMLDKVMFEEIIHPEKWINECYKELMEKEWNNEIERCR
                     RHTERLIKRISNSQYQFPSECPIKGRYPLRDNIGWTEGFWTGINWLLFEKTGDPLFQQ
                     TAEKADIDFIHRIDCQIITNHHDLGFLYSLSTVADYKITGNRKMKEASIDAAETLLQR
                     FWEKAGVIQAWGDPSDPSQQGRMIMDCCMNLPLLYWASEETGNQKYKKAAQAHIQNAA
                     DYLVREDGSTYHTFYMDVKDGKPRYGSTHQGYSDNSCWARGQAWGIYGFALSYRYLKE
                     QKYFDLSKILANYFMNRLPEDTICYWDLIFDDSSREEKDTSSMVIMVCGLLELIESGL
                     LDKTEKNLYQSAVWKIMRQLKERYATTEEEDGFLTQSVYNKPKNNGVNTPCIWGDYYY
                     LEALVRMTSNWSSYW"
     gene            complement(18872..20743)
                     /locus_tag="ACG842_001122"
     CDS             complement(18872..20743)
                     /locus_tag="ACG842_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2264 domain-containing protein"
                     /protein_id="HFQ0247681.1"
                     /translation="MKHPVPLLRTRQDYFDLFKKINQPLQPFYRASGAQIDYIHQGVG
                     YGNKIAGMEGFSRVLWGAGPAIDQLDERWLKLILSGIKAGTDPSHPDYWGEIHERDQR
                     MVEMPAMLLALYHHEQLLWKKLDTGEKSQIIHWFSQIFEYECADGNWQFFKIIVGTII
                     QKFGYPVKESALKEAFDNIENCYLQNGWYRDSSRGRQDYYNPFAFHYYGLIYSVLEPE
                     KPISQIYKNRAIDFSRDYIHFFAEDGANVPFGRSMIYRFAVSSFWSAMLWADLLPFKL
                     GEIKGLVNRNIRWWMQKEIFDEKGILNIGYTYPQLTLTEPYNSTLSPLWLNKIFLLLA
                     LPEDHEYWTAKEMPMPIREGTKLLSEANLLIQHDNGHTFFLNAGQLGPNFHTLTNEKY
                     LKFAYSSQFGFSIPRANQLKAEMAMDSMLGIQRADTYITTSFKNESIKTYGQFIVRNN
                     VRDIVCKKEVLASTWEPTLQAKIRTWLIPFEGWQIRVHKVELDTEYVLYETGFAIECS
                     PEKEGIIIEEDKENYRVSEAGFSGIICLSDDTIKRKSTAIMGLPNTNLMTWENTFIPG
                     LEGTFGKGTHWLGTGVVAHQDRDYSLEVWNNRPKIDFDGTTGLTIQNKNNKKRLKLC"
     gene            complement(20764..21882)
                     /locus_tag="ACG842_001123"
     CDS             complement(20764..21882)
                     /locus_tag="ACG842_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HFQ0247682.1"
                     /translation="MNEQTKEWSRKILNVASEKIDAELDRMGNKIPYISYNGKYTDVI
                     KDRGIDWWTNGFWSGILWQLFHKTKREKFKEAAIQQEQLLESALYHFSDVHHDVGFLF
                     LPSAIAHYQQSQNKHSRQLGLHAATVLAGRYNISGNYLVAWNDHKPGWMIIDSMMNLP
                     LLYWASRETGDPRFSKIARQHADTTAKYLVRKDGSTGHIAEFDAESGKYIRLLGGQGY
                     NENSCWSRGNAWALYGFALSYRYTKNPYYLAIAEKIAVNFIQKIEKTGYIPLIDFAVK
                     DSNIDTSAGVCAACGLLELAGYVSSVRSKMYHEAAINILQKTEKHYANWNMDEDGIIG
                     GGAEAYHRELTYNVPLIYSDYFFIEGLLRLEQSELAIW"
     gene            complement(21965..22798)
                     /locus_tag="ACG842_001124"
     CDS             complement(21965..22798)
                     /locus_tag="ACG842_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0247683.1"
                     /translation="MTKQKIQSRIFTYVIIVFGALIFLMPYLYMIVASTQSNQEILSG
                     GINFKFGDMLLKNWQAIMARYNYPLIIWNSIVIAVISTVSATASSTMAGYALAKYRFM
                     GNKLLFNTVMLSRMVPFFTTLIPLFYIMSRLGLSNTYAGVIIPSIASTTSVFMMRQYS
                     SQFPTALMEAARIDGANEWVIFTKIAVPILSPTIITSGLLIFMGSWNQYLFPLVMLSD
                     KDKYTVPLIIKNMTVSSTGEPINYGAIMLILATSVIPMVLIYVWAQSKFKENDIGAAN
                     K"
     gene            complement(22791..23708)
                     /locus_tag="ACG842_001125"
     CDS             complement(22791..23708)
                     /locus_tag="ACG842_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0247684.1"
                     /translation="MMQSSLPSKTIKNSKKKATDTSHKKSAYGLVTPFVIFFIVTILI
                     PIIISFAFSFMKIGYSFEFVGFDNYLAIFRDPLFIKSYTNVLIIMAGSIPITMLLAIY
                     FAVLLNSAQLKGKGFFRTVYYIPTITSTVAVASVFMTFFNPTGLFNGFLNALGINGVQ
                     WLTNPFWIRISMIITMIWLSVGYNTILFLAGLQGISKEIYESAEIDGASKFRQFFLLT
                     IPMLKPIILMATVLATINGLGNFNIPNIFFGTSNGPENSALVVGVNLYKTSFEMVDFG
                     KASAIAWTMVFVSVILSAIQFKFGGDKND"
     gene            complement(23935..25059)
                     /locus_tag="ACG842_001126"
     CDS             complement(23935..25059)
                     /locus_tag="ACG842_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFQ0247685.1"
                     /translation="MKKRYKLVIALLNITLLFSLSACGKTNEEVTANIADPMIKKATT
                     LEVYVANGKLTNYVENAAKIYNRKTGANIDLKIVNVASGTATVQMITPKLVTHEDMPD
                     IISMADTSAAGVLEKFQDAFYSADEYGFYDKYGDQFYDQKLNILRQQTSNETIIPWAS
                     DFTPALCYYQPKKFAEIGVDFEEISSWEEYIQVAKALKEKTGSFGIALPESGDQELFI
                     NMLAQQNQSLLDEEGNIRLNTKEAKHAAKLIKEMIDSDIVHFYGAQDAEKAFQESAMF
                     VAGGWYATNMSLNFPDASDKWRMAPLIPFSKENPGKGAVSGGSSFYVPKDAKNPQVAQ
                     QFLTFMLTDEECLANALELGVATSNSLAYQTEAAEKKFDY"
     gene            25242..26150
                     /locus_tag="ACG842_001127"
     CDS             25242..26150
                     /locus_tag="ACG842_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFQ0247686.1"
                     /translation="MVLLLTNNQSSNLVKKTLTYSIDTHLHLSAAPYFRLLINESANL
                     YAEFAHKSIELDNPSLLFIDKQQEVQIFSTQKETQTFIIIQFYFDEQLLAHITGQSFS
                     IDQEITSIITFLKNEQFKIDYLKHSKPAISYDQEINSFFILCQQMSIHLLSLLVTYCL
                     QKIQLSSLPRHYQQELALLTIPSNEKVIPKKKHILYSDELVDPIIRYLEEHLSENFLL
                     EQLAQIFFISPASLKKHFRKVNGKSVISYFKELKMEKAKRLVLENELTYTEIADILGF
                     HSIHHFSSAFKKYTGLSPSKYYLSLK"
     gene            26291..27904
                     /locus_tag="ACG842_001128"
     CDS             26291..27904
                     /locus_tag="ACG842_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFQ0247687.1"
                     /translation="MKVVVIGGVAGGPSFATRFRRLNEAHEIIIYERGENISYASCAL
                     PYYLGGVITDRDSLIERTPEILKTKNNIDVFTKHEVTAIDPSTKRLTVKDLSTNEEIK
                     TDYDKLIISSGARPDYPDIPGVFEAENGFVLRSVTDADRIKSFLEEKNPQHVVILGAG
                     VMGLELAENFKHRGLDVTLIDQLPQVAFPYDPEIANLVYDKLLKEGLAVHLETRVTEI
                     RDKGREIVLSDGSVLSADMLIFAVGVSPKNEVVKATGIQLSDTGQIIVDDQLQTNLPD
                     IYAIGDIIETTSVVTGQPIQSMLSSAANRQGHMLADILNGTPMRYRGYIGAGVAKIFD
                     HTASYAGMTEHELKASGITNYKTVFITPFDHAYFYPEATRLNLKLIFDADSGRILGGQ
                     AFGEKGVDKRMGELSVAITGNLTVFDLPDLELPYSPPYSTTRDPLNIAGYVAINQMTN
                     IVETIKASDIPENDLKEAFFLDIREPNKAPSGSISATKNIPMNELRDRINEIPKDKKV
                     YITFRRGLNTYTSARILAGLGIKAILIEE"
BASE COUNT         8846 a         5597 c         4854 g         9160 t
ORIGIN      
        1 taaataaaat ttattatacc tgctcttttt gactatatcc tcatctgact cctatacttc
       61 gtttgtatta aaacttagga ggaatcattc atgcttacag ggctaaactt attaaataat
      121 ccttttctaa ataaaggaac agcttttaca aaagaagaac gggcaaaata cggaatcact
      181 ggtatgcttc caagtactgt ccaaacattg gaacaacaat ctgtccaagc ttacggtcaa
      241 tacttaagca aacagaccga tttggaaaaa cgtatcttct taatgaatct attcaatacg
      301 aaccggacat tgttttataa attgatgggc gaacatttag ttgaattcat gcctgtcgtt
      361 tatgatcctg tcgttgcgga ttcaatcgaa caatacaacg aaattttctt gaaaccacaa
      421 gatgctgtat ttctttcaat cgacgaacca gaaaatatca aagcaagttt gaaaaacgca
      481 gcagatggac gcgacattcg tttgatcgtt gtgacagatg cagaaggaat cttaggtatg
      541 ggtgactggg gtgtgaacgg tgttgacatc gctatcggta aattgatggt ctacactgcc
      601 gcggctggta tcaatcctgc acaagtcttg cctgtatcaa tcgacgctgg tactaataac
      661 gaagaattat tgaataatcc attatactta gggaatcgtc atgctcgtgt agaaggcgaa
      721 acttactacg aattcattga tcaattcgtt caatcagcta ctgaattatt cccagaattg
      781 ttattacatt gggaagattt tggacgagga aatgctgcaa cgatcttaga aaaatacgaa
      841 gataaaatca ctactttcaa tgatgatatc caaggcacag gaatcgttgt actagctggt
      901 gtattaggtg gattgaatat atcaggtgag caattgaaag atcaaacgat cttgacattt
      961 ggtgctggta ctgctggtgt aggtatcgca aacatccttt tagatgaaat gatccgtcaa
     1021 ggtgtacctg aagaagaagc acgccaacat ttctatcaag tagataaaca gggtttattg
     1081 tttgaagata caaaaggatt gactcctggt caaatcccat tcgctcgtaa acgctcagaa
     1141 ttcactaatg ctgatgaatt gacaaactta gaagcagttg taaaagcaat ccatccaacg
     1201 atcatgatcg gtacttctac ccaacctgga gcattcactg aagaaatcgt gaaagaaatg
     1261 gcggctcata ccccacgtcc aatcatcatg cctttgtcta atccaacaaa attggcagaa
     1321 gcaaaagcaa aagatttgat tgaatggacg gacggaaaag ctttagttgg aacaggtatc
     1381 cctgctgctg atgtggaata taacggtgta acttaccaaa tcggtcaagc aaataatgcg
     1441 ttgatgtacc caggtttagg gcttggtttg attgcttcaa cagctacacg agtaaatgct
     1501 gaaatcattt ctcaagctag tcgagcatta ggcggaatcg ttgatgtaac aaaaccagga
     1561 gcagctatct tgcctccagt agcaaaaatc actgaattct ctcaaacgat tgctgaaact
     1621 gtcgcaaaat cagttgttgc acaaaaattg aatcgtgaag aaatcacgga tatcaaagaa
     1681 gctgttgaat ctgctaaatg ggttcctgaa tataaatctt tagaagatta agattgttat
     1741 agtaaaagaa catacagaaa ctttgtatga cgtctaaaaa gaaaaaagcg actgcggcaa
     1801 aacagcagcc agccaactac tgctcttttg ccgcttctct ttggcttttt tcatacgata
     1861 cagaataaga aaggatctgt aaaaatggca caaaaaacaa caaccgcaac tgcagttgag
     1921 gaaaagaaaa aagaagggtt ttggtcaaca aaaattaccg gcgtaagttt gcccctttat
     1981 ttgatcatgg tccttgtcct gatcattgca atggctttag gaaaattacc gacgaatatg
     2041 attggcccaa tcgctgtttt ggtcattttc ggtaatttat ttcattttat cggaacaaaa
     2101 atccctatag tcaaaagtta tttaggtggt ggctccgttt ttgcgatctt tgcctctgcc
     2161 gcgattgcta catttgggat cttacctgag tatgtagtaa aatccacaga aaactttgtc
     2221 agcaatatgg gctttttaga cttttatatt gctgccttga ttactggtag catccttggt
     2281 atgaaccgta atctattgat gaaagcttct gttcgtttta ttcccgttgc acttatttca
     2341 atggtcgtct ccttttttgc cgtaggactc gtaggtatgc ttatcggaaa tggttttgca
     2401 aattccgttt tatatatctc attgcccgca atggctggtg gtgttggagc cggagctgta
     2461 cctttatcaa cgatttatgc aaatgtttta ggtacggacg cttcttctat catttcccgc
     2521 ctgattcctg cgacagcaat gacaaatgtc ctagctatca ttggtgcagc gttagttgct
     2581 cgtgcgggac aatcaatgcc aaaattcaac ggtaatggta aattaatgga aaaaggcgac
     2641 cttggcgatg gaaaacctag aaatgtaaaa ccttccattc cacaattagg tgtcggtttg
     2701 atggtatcat tgactttctt catcttagga caaatctgta actattttgt tcctaaagtc
     2761 catgcttacg cattcatgat cattatcgtt gttatctgta aaatcgcaaa cctattacca
     2821 gaatattacg aagatgcagc tatcatgttc aacaacttga ttgtcaaaaa cttgacagca
     2881 gctgttcttg ccggtatcgg tattgcgtta ttgaatctaa atgtattggc ttctgctttg
     2941 acatggcaat ttgttgtctt atgtttgaca agtgttatcg cgatttcagt agtttcagga
     3001 tttgtcggtc gtttgtttgg tttatatcca atcgaatcaa ctattactgc tggcttatgc
     3061 aacaactcga tgggtggtac aggaaatgta gctgtcttat ctgcagctaa tcggatggaa
     3121 cttattgctt ttgctcaaat gggcaatcgg cttggtggtg ccatcgtttt gatcatctca
     3181 ggtttcttga tgcaattact ttcttagtca aaaaaaatac ctgatcatca agtatcggct
     3241 tgatgaccag gtattttttg ataaaaattt acaggatttt caaaaatctt ttttgcattt
     3301 tatcggaatg ataaccgatg cgttcataaa atcggtgtgc ttctactctg tattctgcag
     3361 aattaagtct tattgctgat attcctcgtc ttttcgcttc ttctacgcca gtcatcaagg
     3421 cctttccgat tcctcttttt tcagcatgtt tagacacagc aagtgccata atattgaaca
     3481 tcggatcgct gaaaatcgat tcataggact ccgcttgtac atagccagct gcttttttag
     3541 aagtaggatc ttcatagacc aagaaaaaat ttttttctgg ctccttcaac agtttcttca
     3601 tttgtctcac cgtaacggtt aatggtacat catatcctaa ttgttccgca ttgatttctc
     3661 gtagtgcttc tgcatctcct aaacgcattt cccgcaacat ccaaatcacc agccaatttt
     3721 tattttttta aatgaatttt tttatcacag tatactcctt ttttcactta aaaaaagact
     3781 tttcagtgat tttgacttgc ttgaatccag atccttctaa tgtttgcaag atgacgaaag
     3841 cattgtaaag attgatcaaa agaaccacga tgacaatcgc ttgaaaaata aaggtcactt
     3901 tttttggaga caaaaccctg ctaaatcttg ctccccacaa acctccaata acagcagcgg
     3961 ggataacata aaacaacata gataaatcaa agcgcataaa gctacttgtt aaagcaatcg
     4021 ttaccaattt tgccagttga gaaaagaaaa tcgtagatag agaatatagt gttgcttctt
     4081 ttattggcaa tgcaaacatc agcatcaaca aagaaacatt gactggtcca ccaccaatac
     4141 ctaaaaaact agctaaaaag cccagaaccc ccccacatat aaggtaccaa gtccatgacg
     4201 tcaaatgaaa ttttggctga tgatgcttgg tgtagaagaa cgcaaaaacc aaggtcaata
     4261 ccgtcaaaaa aatttggatc agctgcacat gcttctcgtt ttcaaataac tgtaaaaaaa
     4321 cttcgaatac aatgttgccg actactcctc caacaacagc tccactagat acccaaccga
     4381 caatctgcca attcagtgat ttcccgcttg atacttgtcg cacagtcgaa acaatggaca
     4441 tcgtgaatac cgcaactgtt gagtaaaaag aaatccctgc aacagaatgg gcgccgataa
     4501 gatcaagaat cggtttgatc aacacgcctc cacccatccc ggaaatagcg ccaactgtgt
     4561 tcgccagaac gatcaccaca aaataaatca gaccaatcat cttgtatttc ctttctcttg
     4621 tttatatctt ttagcgattg ttactctttt cttttattat acagtaaaag aaacaaacga
     4681 ggcttcgaaa gcttttctac tttttaagga aatttagtgc taaacgaaaa aaccgggctc
     4741 tacttttcag agagtccagc ttttcttaca ccttatcgat tcttttgttc tattcttctt
     4801 ccgccatgaa tgtattgaat acttcttcga tcatatccca ttctgcgtct gtttcgattt
     4861 gttctaactc gccttctgtt ccatcttcgt tttcgatgta tgcataagct tgtaattcta
     4921 catcttcatc ttctgaaaca ccagctggat ataaaagaac atagttacgt ccaaattctt
     4981 cttgtccgtc aactgttagc aagatttcgt ataatgtttc atttccttgg tcatccacca
     5041 atgtgatgtg ttcgtgtcct tcgtgttcgt gggaatggtt atgttctgtc ataagattac
     5101 ctcttttctt tttattatta aaataattgc ttacctgagc cgtctaaata attttgtaaa
     5161 atcatcacag ctgctaattt atcgatgact ttttttcgtt tcgctcttga agtatttgcc
     5221 tgctctacta gcatacgttc tgcctgaaca gtggttagtc gttcatcttg ataatcaacc
     5281 ggcaaggaga atagttcttt gatcttctct ccatatgaca tggaagcttc tgcccttggt
     5341 ccgatggaat tgttcatatt cttaggtaat ccaactacaa aacggtctac ttcgtactgc
     5401 gccactaatt ccttcaaccg atcaaaacca aactcttctt ctgcctcatt gattcgaatg
     5461 atttctactc cttgagctgt ccagccaaag ggatcgctca cagcaacgcc tactgtctta
     5521 gaaccgacat ccaagcccat tactctcata aattcactcc atgatttgag aggtagtctt
     5581 tgacaattac ttccatgatt tcatcgcgtt cgtgacggcg aatcaaattt cgtgcatcac
     5641 gataacgagg aatgtaggcc gggtctccag aaagcaagta gccgacaatc tgattgattg
     5701 gattgtagcc tttttcttcc aaagcccgat aaacaatcgc taatgtttcg ctcacttctt
     5761 ttttgcgact atcgtcaaaa ccaaaacgta cggtttcatc tgtaaaaccc atgattctac
     5821 acctctttcc cgcaaggtaa ttccagctta ccttctacac ccttatttta ctcgaagaaa
     5881 gggaaaacta caaacaaata ttccaaactt ctgaataaat taagaaacac gatttgtctg
     5941 atttttcctt ttttctgtgg ctttttcaaa agccctcttt ccataaagta tacgctatat
     6001 ttctttgaaa gtatgttttt acacaatttt ttcataaagt tcataaaaaa ttcgaattgc
     6061 tgctactaat ttttcgtttt cgttgcttgc cctgtctcat aattgatggt gtagccatct
     6121 tgaacattgt agataaagac attgaattca atttttttgt cttcaatgct ttctgcttcc
     6181 aactgcacac ctcttgctac cagttcattt ccctcgaaat gaggagtgac acgatagcgt
     6241 acatgatgat ttgtttcctt gatatatgcc gcaatatcat tttcgtgggc aagcatgtac
     6301 ggagcattca acgaacgtgt ccctgtcatt aagtttttaa tgttattatt ttgaccagtc
     6361 aactggaaac caatcaaatg gctgcggttg tataaccatt gtccgtcact taatttcttt
     6421 tgtttccaac ctgtcggatc aatatacaaa ggttctctct tttcagtagg aaacaagtct
     6481 tttcccaaca tcgcattggc ttgtcctact ctgttcaatt tgtctatatt tgaatagctt
     6541 tcccatgtcc ctttggaaag actaagatcc tcttttgtaa actccggctg attcccattg
     6601 atttctattt cttgatgttt tccatcatat tctggcacat ccgctacttg agaagcttgc
     6661 ggtgtggaat caaaaatggt tgtaatttgt tggaagatat ctgaatcttt ttgtgtggtg
     6721 gaaataccga gtgcccctgc aatcacagca gcgacgatcc ccacgatcgc cttggttgtt
     6781 tttttgctca tttctttact cctttactaa cagttcccta aatcttaaca tatgttcccc
     6841 tataaataaa tactggaaag aaatagaaac agtaaaaaaa taaaaccagt ttttagacgg
     6901 cttaagaata caaaaaaaga gacggattca tcgtctctac tcaatcacaa aacactattt
     6961 acaaggtatt tattatcatt caaaaaattt ttcttaatac tcaaaactaa acggcttctg
     7021 tacgacctct tggtaaacag tgttcaaaag cttgcatctg cggtaataag tccaaccaag
     7081 tcaaaaacat aagaagcttt tttgcttgac tattcttatt acttgatgca gggcagcttt
     7141 ttcacagcct cttttaagcg ggtgacggga atcgaacccg cgacatcagc ttgggaagct
     7201 gggattttac cactaaacta cacccgcgat acaaaaacag tatacgcctc tttcactcct
     7261 tcctcaagtt tttttattct ttacgcttcc gatatgtttt gtagtaaaat aaaactaatc
     7321 aatcaattat tggagaagat gctatgacgg gaaaagaatt agatctgttg ctggacactt
     7381 gtttactcgc tgggaaaatc atgatggaaa gcaatgcgga gatgtatcgg gtagaagata
     7441 cgatgagtcg aattgcttta gcttctggaa atttccgttt ggtcagctat gtcactcaaa
     7501 ccggtctctt tattggtctt gatcgtacct cgaccatccg aatggaacaa attaccaacc
     7561 gttcgattaa tttagaaaaa gtagtaaaag taaatgatct ttcacgtaaa tatgttgctt
     7621 cagagctaac attagaagaa ctttactgcg aactgcaagc aatcgaccga gaccgttctt
     7681 tctttcctgt ctggttgcaa atcgttagcg cggcagtgat cagtggaaca attatggtgt
     7741 tatttggcgg aaagatgacc gatttacctg tgactttagt cattggagga ttgggatacg
     7801 cagcttatct atatagtttg aaatttttta gaatcaaatt tttatcggaa tttttatctt
     7861 ctttattgat tggcatagcc gctatctaca gcgtacgttt tggcttagga agcaatcagg
     7921 atctaatcat tatcggatgt gtgatgcctt tagttcctgg agttcaaatt acgaacgcta
     7981 tccgtgattt gctagctgga cattatgtct caggagtttc tcgcggaaca gaagcgatga
     8041 tgactgctac gatgatcggt tttgcgattg cttttatttt ccagcttttt tattaggaaa
     8101 ggaacacaag tatggggacg atatttattc agttttcttt tagtttttta gcgactgcgg
     8161 catttgccgt tattacaaat gtacctaggc gttcattgat ttgttgtggt ctttctggtg
     8221 cttttggctg gatgatctac tggatctgtg ttcaaatagg cggatctgct gcttttggtt
     8281 cattgcttgg ttctttagga gtagcattca tcagcgatct tttctctaaa cgtttgaaaa
     8341 tgcccgtaac gattttcaat attcccggga tggttcctct agtccctggt ggtttagctt
     8401 atcaagcggt tcgaaatctc gttactgaat cctatcgaga agcggctcat tatgcggtcc
     8461 aagcaattat gattgctgga gcaatcgctt taggtcttgt attatcagaa gtctttaacc
     8521 ataacatccg taatttcaaa gtaaagcgtg acgcaatcat gatgaaacga aaaaaacggc
     8581 tttttaaata aacgaagtgt aacaaagaaa aggttgtgaa agagacgttc agccttgagc
     8641 gatgacaaaa tatctcacaa acattgcgac aagtaaccgc aggagcaata agaagtaatt
     8701 atgagtatta aaaaaagttt tgtaaaaata gcttctcaca tttttacata aaattgactt
     8761 aatactgaga aactgacttt tgaacaccct ctatctgtaa aaaaggggct gcgacatgat
     8821 ttttgtcacg gcccctcttc tttattcaaa ttttttccaa aacgttcgtt gataataaac
     8881 gatccataga gcggaaagta ttagaactaa accgatcaca ggaatattca ctaaccaaaa
     8941 tggataaaac aaagccaaca ttccgtataa gacgataaga ataatactga tgatcaatga
     9001 acccatcata gttgcgacta aacccaaact tcctgaacct ctgttaaata attggttaat
     9061 attggtccag ttcagcagca gcaaccgata atctctagcg aaataacgta gtgacaataa
     9121 gtagcagccg aataaagaac caaatagcaa gctagggata aaaaacaaag gcagctgaaa
     9181 agacagtccg cttaataatg cgatcccacc agttaaaaaa ctctgcaata tccatccgaa
     9241 tctgaacttt tcttttaaat attggttcat agagattggc aaagaacgga taaacagaaa
     9301 attttcttga tccaaagaaa taagattgct gataaaagaa gtctgattaa ctgttaagaa
     9361 agccaatgcg atccctacca ggaaaacaac accgattaac ttattatcta aatcagttaa
     9421 ttttatttcg cctcctaggg caaaagcaag aataaaaatg attggagtta acaaggaaga
     9481 agaaagaact tgcataatca aattaggttc ttttactaat tgagcattgt aactaaataa
     9541 aagctgatgc acattttggt tctttttatg ggttcttttt attttgaact cactgtttga
     9601 atcagtcagt tgctctgtaa gctttggtac tatcagctgt tttacagcaa ggaaaaatcc
     9661 cagagccgtg atagctagta ttccccaacc caataaacct tgttgagaaa gtggctgact
     9721 ggcagcatca tagaatggca ataatatcga aatcggatgc cgatctgtta actcattttc
     9781 aatagtcccc gtttgatgat tcatccacat gattccagca aatgcgatca gcatcgagct
     9841 gccaagcaag atactagaaa ctgtttttct atgtttttta aacactgctg ttcgagccaa
     9901 accaaaaaca atcaaactag ccagacaaaa aagaataatc aaaaatagag aaaacaaaag
     9961 gattgagata atgatagtaa gcaaaatccc tagatcagct cgccatccgg ttaaaataaa
    10021 catgactaga agcggaaaaa caaaaggaat gatggtcaaa gcaactacca aaatctttgc
    10081 tgaaaaaacc tgttcttgtc caaaaggcag aggcaaaaat acttgcaggt catttccttc
    10141 aaaaaagata ttataaatga ctgatatact ttgagttaaa cctaacatac caaaaagtgc
    10201 cacgtaataa gtaaagaaac ctggcaattt aacaaagttc atagcaaaca tagcaaatcc
    10261 gtaaatcagt aaaaaaatca gccctgaaaa aagattttga gacaaaagat agatagttaa
    10321 tcgctttccc ttttttcctt tctctcttgc tttattcgtg agctgtggat taacatataa
    10381 aagattaact cgaattaatt ctaataattg tcgcttatac atacggtcca tctcctgtcg
    10441 cttgccttcc agccattttc aaataaatct tttctagcgt ttcatcaggt gagccattca
    10501 ataattcact aactgccccc tgataaatca attcaccttt cttcaaaata gccagtttat
    10561 cgcataactg ttgtgcggta tccaatacat gtgtagaaaa aatgactgtt tttccttttg
    10621 cggcatgagc cttcatcatt tctttcaaat caaatgctgc ttgaggatca agtccttgca
    10681 aaggttcatc caatacccaa atatctggat ctggcagcaa cgtgccgatc aaaatcgttt
    10741 tttgacgcat tccatgtgaa aaacttgcga ttgtttcatt ttgatgacta tacatatcaa
    10801 aaagcgctgt caattctgct aaacgctttt cttttttttg tgtgtctaat tcataggcag
    10861 ctgaaatcaa gtcccaatat tctccagccg tcaattgtaa aaaaatatct ggggaatcag
    10921 gcacataacc gattttttgt ttgatactca atcgattttc cgtcaatttc attccatcta
    10981 ctaaaatcgt tcccgatgat ggttcaataa tactaactaa acttttaatc gtagttgatt
    11041 ttccggcacc attatgtcct aaaaatccaa aaatttcccc ttgtcttatc gagagagaca
    11101 agtcttttaa tgcttgtttt ttcccgtaac ttttacttac attgatgaat tcaatcattt
    11161 ctgtactcct tttgtttgtt attacttttt attgtaccct tcgttattgg tggagtaaat
    11221 gtcaaaaaga gtgcataaca aaaaaggata gaaacgacta ggctgctact tgggttgttc
    11281 tcttctacag gaaaacactc ttctaacagg taagatatga gtctttgttt tctttaaaat
    11341 gattcatttt ctatcttttc ttgcataaaa ttctttagtt tatcaatact tctctttccc
    11401 atctctagtt gttctccatt tgcaaatgtt actatttcgt ttactctgct aatagatgta
    11461 atgttttcag tattaatgat ataagctttt aaatctttac ataaatactc atattcgagt
    11521 gattttttat attttttaat tgtgccgttt acgagaatct cttcatcttt caatactagg
    11581 cctattttat ttttataacc tggcatgctc attagataaa ggatttcatc gtaatggata
    11641 tagtgatcgc tattaccata acggacagtt gtccaccttt ttttcgtagc atgttgaaca
    11701 ggatagtttt ctttttcttt caataagtaa ctttggatgc tttttttatt tttttcataa
    11761 gtgaattctt ttatcaaata accactaggc gtaatctttc gattaataac agttattgct
    11821 ttattcggat gagacgttag ataaacaatt ttacactttt cattcttttc tctaagcatt
    11881 tcgcctagtt cgataccgct ataataggcg ttcaaatcga tgtctaggaa aaaaatatct
    11941 gaggaataga tttcattatt tttaatgaat tcaacaaatt gtataacgtt caatccctga
    12001 acgatctcaa atttccaagg acttaataaa attctggatt cttccaaaat ttctactagc
    12061 attttttcaa aaaccaaact atcatccaaa ataaatatac gcgttaccat aaaaagtacc
    12121 tccactcgat attttactat caaaatcatt tcatacaact tttcaacata catgttagga
    12181 ataaattgta attctcttca caatcttttc caacaccttt gaaaccgata tcaaatactt
    12241 tcaggatgct gcttctaaaa taaatatcaa acaaatgaat acatcatgca aaaaaagcca
    12301 gacaaccgac gtccttgcgt gtcagctgtc tgagctatgt ttatttattt tgttctttta
    12361 aaaagaacga tacacatact gatttttcgg agcagagact ttctcagcgt ttgtaacaac
    12421 tacagatggg atttcacgct tgaatggttg ataatagtct gattggatgg ttccttctat
    12481 tttataccat tcatcatttt taaccgttac tccttccggc atatgcgtca acaaaccaaa
    12541 gactcctgaa tctgccacac aatggataat gccgaatcgg aacaagaaga tatctgtatt
    12601 gctatcgttc ggtgtttggt aaacaaatcc ttcatagctg atttttttgc caatgaattc
    12661 gctagggtaa ttataaatta gctccataac ttctaaatag ttttcatctg tgatcttgat
    12721 agtttgctta tccccatact tttctttcaa ctcattcatt tggtctaagt agtctgattt
    12781 gttgaaataa atactggtat ccggctttaa gtattgtgtc tgcatctccg gatcccctac
    12841 tgattcttta cttactggga aattaaatcc tttggcttct acgatcgtag tgtcgagact
    12901 aactgttgga aacaacgtcc ccacgattac tggcaaagct agcagtacat aagcgattcc
    12961 acgttgataa ggttttgaca acccatggtc atgatcgtgc tcgtgtgcct tttcttctgc
    13021 ttttccgttt ttgacccaca aatacagctg aactaaagcc aaaagagcag ataaaatcat
    13081 tgaaagaata gctaagtagc gataatggac attgatatat tgatcgagtt tgccagaaac
    13141 ttgcaagtac atcatcaatt cgaaataccc agataaaatc aaaaaccgca gcatattttc
    13201 cctcctaaat aaataatgtg aatccaataa cgctaagggc aatcagtccg atcagcgata
    13261 aaataaagcg tcctgtaaaa tatcgtttca tcatcagtag atttttgata tcgaccattg
    13321 ggccaaagac caaaaaggca gctacaggtg ctgtgccgaa cagacttaac aaagaagatc
    13381 ctataaacgc atctgcttct gagcaaagag acatcgtaaa ggccaataac agcatgatga
    13441 taatggctaa aatctttgtt gagccgagcg ttagcataat tctagttggt aaataagtct
    13501 gcatcgcagc tgcgattaat ccgccaatga tcaaataacg acctgtatca aaaaattcgt
    13561 cgattgaatg tgtcaacaca tgcccagttt gatggatcag ctgatttttt ggttcattct
    13621 tctcactttg ttcttcttca tggacatgat gatggtgatt ttctgctact ttagacgtga
    13681 ttttttcttt caatacagcg tctttttgaa aataggcaag ccagacacca atcacaaggg
    13741 caacaaaaaa gctgcctatc acccgccagc ttgccatctg ccaagaattg ccaaatgcga
    13801 taaaagtgga aaaaatcacg attggattga tgatcggagc agtcagcata aaagcaaacg
    13861 ctgtgtgaac tggtacgcct tttttaacaa attgatgaac gatcggtacg atgccgcatt
    13921 cacatgaagg aaagaaaaat cctaagatcg aaccagtaaa aatagagaag atacgatttt
    13981 caggaagcca tctcttcaca cgttctgggg tcaaaaatac ttgtaaggcg cctgagataa
    14041 gacagcctaa taatacgaaa ggtaatgctt caataacgat cgataagaaa atcgtactca
    14101 tctgcaaaac cgaatttgga ataaaagaaa acatgagttt cctccttttt atttctagtt
    14161 cattcgcttt gcaaatatac gcttttattc aaaaaaaaca agttgctctg taaattttta
    14221 cacaactctt attttttacc aaagcacttc aattgacttt ttctgttatt cccattaaat
    14281 taaggtctca tacatatcac ttctatcata aaagggagaa taaaaatgaa acaaaccact
    14341 aacttgactg aagttcaaga catactgcaa cagtcatccg ttgccgtcat ttatctttcg
    14401 atgcctaatt gcagtgtttg tcacgctgtt cgtccacgac tagaggaact tctcacacat
    14461 tatgatattc cggcacttca tttggatgca tttgaaacac cagaagtagc tagtaaattt
    14521 gaagtgctga ctgcgccagt tgtcctgatt tttcatcaag gaaaagaagt ctttcggcaa
    14581 gcacggttca tcgactttaa aaaaatccgt tatctactag atgaactgac agcagaagat
    14641 gattcccttt cttatgaaga aatcttccga acaaaataat cgttgtccaa acagaaaaaa
    14701 gcgattgaac aagtgaaagt tttgccttca tttgttcaat cgctttttat ttttattctg
    14761 ctgtattttc ggaatgcgca tttgcgtcag aatgcaagaa atagatcaag gtttgcaatt
    14821 cattcgtcaa atcaacattt tggatcaata catctgacgg agcattcaat cgagcagcgg
    14881 taaagttcaa gattcctttg attcctgctt ccgttaactg attgactact tcttgtgctc
    14941 tttcagcggg tatcgttaaa attgctactt cgatttgttg gatcttgatt tgttctgaca
    15001 tatcttccat tggataaacg gggataccgt ctacgatgcg cccaacgatt tcctcattca
    15061 catcaaaagc acaactgata cgtatgctat tactttgatg gaatttgtat tttagtaaag
    15121 cacttcccaa gttccctaca ccgatcagcg caacatttgt caattgatcg tcgtttaatg
    15181 tctttgcaaa aaagtgcata agatcttcta catcataacc atacccacgt tttcctaatt
    15241 cgccaaaata agaaaaatct cgacggatcg ttgcactatc tacttgaacg gcttcactta
    15301 attcagttga agacactttt ttctttccag cattatttaa aatccttaaa tagcgataat
    15361 acaaaggcag tcttttagct gtcgcctttg gaataatatg atctttcacg aaatgacctc
    15421 cacgtattgt ctaattacat ctttgatttc tccatcatag cagtatttat aggaaaaaat
    15481 caattccatt gctcagatat tgtgaaattt tattctttat tcatttattt atcatttctg
    15541 caaatctcgc agtataatgg aatttatggt agactactaa ttgaaaaata gttttttatt
    15601 tgaggagaat tcacaaagta gaaaagagga ttccatgata ttattacaag ccaatcagat
    15661 tgctcgccat tttggtgcag aagtcttatt tgaaaacata catttagaaa tcagtacagg
    15721 cgctcgtgtc gctttagttg gcagaaatgg cgctggaaaa tcgactttat taaagatcat
    15781 tgctggaatt gaagcacctg atgaaggaaa gatcgctaaa aataaaacag cgactctcgg
    15841 atacctcgct caggacacag gacttgattc caatgaaaca gtttggaacg agatgctgaa
    15901 agcttttgaa caagttagac aaatggaaga gcggatgcgc gagctagaga ttttcattag
    15961 cgaaggtacg ccagacacac ctgcttatag ttcttattta aaagaatacg accggctgca
    16021 acacgatttt tcagatcgaa acggctatgg ttatgaaaat gaaattcgtt ccgtattgca
    16081 tgggtttaaa ttcgattctt cattttatga tcagaaaatc gatactttat caggcggaca
    16141 gcgcacacgt ttagctttag ctcgcatgct tttgcaacac cctgacattt tgattctaga
    16201 cgaaccaacg aaccatttag atatcgagac actttcctgg cttgagaatt atctgcaaag
    16261 ctatactggt gcattattga ttgtttccca tgaccgttat tttttagata aagtagtcaa
    16321 tgaagtctat gaattaagca gaagaaaaat gcgccattac aaaggtaact attcgaaata
    16381 tctagatttg aaagcagaac agttagcaag tgaatggaag gcttatgaaa agcagcaaac
    16441 agaaatccat aaattagaag acttcgttgc tcggaacctt gttcgcgcct ctaccacaaa
    16501 acgagcgcaa agccgcagaa agacattaga aaaaatggac cggctggatc gtcctcaagg
    16561 ggatgaaaaa caagcaaact ttctattcag tatagaaaag ccttctggaa atgtagtctt
    16621 gcaaatcgaa gatgccgcta tcggatatga taatgtcacc ttatctgaac caatcaatct
    16681 agatatccgt cgtcaagaag cgattgctct agtaggtccg aatggtatcg ggaaatcgac
    16741 gttgctgaaa tcaatcattc accagatccc ttttatccgt ggaaaagaaa cgctagggac
    16801 aaatgtcacc atcggctatt acgatcaagg tcaagctgat ctgcatacaa acaagacgat
    16861 cctagcagaa ttgtgggatg agcatcctac cactccagaa aaagagatcc gaagtgcttt
    16921 aggtgctttc ttattttctg gcgaagatgt agaaaaacca atccctctgc taagtggcgg
    16981 tgaaaaagca cgggttgctt tagccaagtt agcgatgaac aaagaaaatt ttttgatttt
    17041 ggatgaacca acaaaccatt tagatattga caacaaagaa gtattagaaa atgcattgat
    17101 cgattatgac ggcacactgc tttttgtttc ccatgaccga tatttcatca atcggatcgc
    17161 gacaaaagtc gtcgaactct ctgaaaatgg cagcaaagtc tatctaggag actatgatta
    17221 ttatttagag aaaaagcagg aagaagaaga aatcgccgaa ttgttggcac aagatgagcc
    17281 agaatctgct cctgctccca cgaacaaatc tgattattac caaagcaaag aacagcaaaa
    17341 gcttatccgt agccttcaac gaaaaattac tcaggttgaa gaagaaatgg cgcggatcga
    17401 tgaatggatc gatcagttgg aacaagaaat ggtcgcgcca gaaaacctgg aagatcacgt
    17461 aaaactcaat gagctgaacc aagatttaga agctgctaga caagatcaag aaacaaaatt
    17521 gacggaatgg gaagaactaa gcttggaatt agaagaatat gaaaattaac gaaaaaacca
    17581 aaacagaaac cattcatgct gttttggttt ttcttttttt attttatcaa acaaaaattc
    17641 catatttacc tttttcacca ataagaagac caattactag tcattctcac taaagcttct
    17701 aaataatagt aatcacccca aatacaaggt gtatttaccc cattattttt gggcttatta
    17761 taaacagact gtgttaaaaa gccatcttct tcttccgttg tagcatatct ttctttcaat
    17821 tgtcgcataa ttttccaaac agcagactgg tataagttct tttctgtttt gtctaataga
    17881 cctgattcta ttaattctaa taatccacaa accatgatca ccatactaga tgtatctttt
    17941 tcttctctgc tagaatcatc aaagatcaaa tcccaataac aaatggtatc ttctggcaac
    18001 cgattcataa aataattggc taatattttg cttaaatcaa aatatttttg ttctttcaaa
    18061 taacgataac ttaatgcaaa accgtaaatc ccccatgctt ggcctctagc ccaacaggaa
    18121 ttatcactat aaccttgatg cgtacttcca tatcttggtt ttccatcttt gacatccata
    18181 taaaatgtat gataagttga accatcttct cttactaaat aatctgctgc attttgaata
    18241 tgagcctgag cagctttttt atatttttga tttccagttt cttcactcgc ccaatacaat
    18301 aatggaagat tcatgcaaca atccatgatc attctacctt gttgtgaagg atcactagga
    18361 tctccccaag cttgtataac tcctgccttc tcccaaaaac gctgaagtaa tgtttctgca
    18421 gcatcaattg aagcttcctt catttttcga tttcccgtta ttttataatc tgctacggtt
    18481 gataaagaat acaaaaaacc aagatcgtga tgattagtga taatttgaca gtcaatcctg
    18541 tggataaaat ctatatctgc tttttcagct gtttgttgaa ataaaggatc acccgtcttc
    18601 tcaaataaca accaattaat tcctgtccaa aatccttctg tccaaccaat attgtctctt
    18661 aatggataac gtccttttat aggacactcg cttgggaatt gatactgact gttactgata
    18721 cgtttgatta acctctccgt gtgtctgcga caacgctcga tctcattatt ccattctttt
    18781 tccattaatt ctttataaca ttcatttatc catttttctg gatgtattat ctcttcaaac
    18841 atcactttat caagcatttc cccaccagcc tttagcataa ttttagtctt tttttattgt
    18901 ttttattttg gatagttagc ccggttgtcc catcaaaatc aattttggga cgattgttcc
    18961 atacttctaa actataatcc cgatcttgat gagcaaccac tcctgttccg agccaatggg
    19021 ttccttttcc aaatgtaccc tctaatccag gaataaaagt gttttcccat gtcatcaagt
    19081 ttgtattggg caaacccata atagctgtgg attttcgttt aatagtatcg tcacttaaac
    19141 agataatacc tgaaaagcca gcttcactca ccctatagtt ttccttgtct tcttctatga
    19201 ttattccttc tttctcagga gaacattcaa tagcaaaccc tgtctcatat agaacatatt
    19261 ctgtatctaa ttctactttg tgtacgcgaa tctgccagcc ttcaaaagga ataagccaag
    19321 ttctgatttt tgcctgtaaa gtaggttccc atgtagaggc aagtacctct ttcttacata
    19381 caatgtctcg tacattattt cgaacgatga actgtccata ggttttgatg ctctcattct
    19441 taaaactggt cgtgatatat gtgtccgctc gctgaatccc tagcattgaa tccattgcca
    19501 tttctgcttt caattgattc gctcgcggta tcgaaaaacc aaattgacta gaataagcaa
    19561 attttaaata tttttcattg gtcaatgtat ggaaatttgg ccctaattgt cccgcattca
    19621 aaaaaaatgt atgtccatta tcatgttgta tgagcaaatt ggcttctgaa agaagtttag
    19681 ttccttcacg aataggcata ggcatttctt tagccgtcca atactcatgg tcttcaggta
    19741 gcgccaacaa taaaaaaatc ttattcagcc ataatggaga taacgtacta ttatagggtt
    19801 cagtcaatgt caattgaggg taggtataac caatattcag gatacctttt tcatcaaata
    19861 tttctttttg catccaccat cttatattcc gattgactaa ccccttgatt tcacctaatt
    19921 taaaaggcaa aagatcggcc cacaacatag ctgaccaaaa gcttgaaact gcaaaacgat
    19981 aaatcatgct tcgaccaaaa ggcacatttg caccatcttc cgcaaaaaaa tgaatatagt
    20041 ctctagaaaa atcaatagct cggttcttat atatctggct aatgggcttt tctggttcta
    20101 gtacgctata aatcaaacca taataatgaa atgcaaaagg attataataa tcttggcgac
    20161 ctcttgaact gtctcgatac cagccatttt gaagataaca attttctata ttatcaaatg
    20221 cttctttcag tgctgattct ttaactggat aaccaaattt ttggataatt gttccgacaa
    20281 ttatcttaaa aaattgccaa ttaccatctg cacattcata ttcaaaaatt tgcgagaacc
    20341 aatgaataat ctggcttttt tctcctgtat ctaatttttt ccataataat tgttcatgat
    20401 gatataacgc taataacatt gcgggcattt ctaccatacg ctggtctctt tcatgtattt
    20461 ctccccaata atcgggatgg gaaggatctg ttccagcttt tattcctgat agaattaatt
    20521 tcaaccatcg ttcatctaat tggtcaattg cgggccccgc tccccataaa acccgagaga
    20581 atccttccat tcctgcaatt ttatttccat atcctacccc ttgatgtata taatcaattt
    20641 gtgcgccact tgctcgataa aacggctgga gtggttgatt tatcttttta aataagtcaa
    20701 agtaatcctg tctggtcctt aatagtggta ctggatgttt cattgtattt tctcctcttt
    20761 ttactaccaa atagctaatt ccgactgttc taatcgaagt aagccttcaa taaaaaaata
    20821 atcagaatag atcagtggca cattataagt cagttctcta tggtatgctt ctgctccgcc
    20881 tccaatgatc ccatcttcat ccatattcca atttgcatag tgcttttctg tcttttggag
    20941 tatattgata gcagcttcat gatacatttt tgatcgtaca gaggaaacat atcctgctaa
    21001 ttccagaaga ccgcatgcag cgcatactcc agcactggta tcaatattcg aatcttttac
    21061 agcaaaatca atgagaggaa tataaccagt tttttcgatt ttctgaataa aatttactgc
    21121 tatcttttct gctatagcta gatagtacgg attttttgtg taacgataac ttaacgcgaa
    21181 tccatataaa gcccatgcat ttcctctgct ccaacaagaa ttctcattgt atccttgccc
    21241 gccaagtaac cgaatatatt tccctgattc tgcatcaaat tctgcgatat gcccggttga
    21301 tccatctttc ctcactagat acttagctgt tgtatctgca tgctgccgag caattttaga
    21361 gaacctcgga tcaccagttt ccctcgaagc ccaatacagt aatggtaaat tcatcatact
    21421 atcaataatc atccaaccag gtttatggtc attccatgct actaaatagt tccctgaaat
    21481 attatagcgt ccagcaagta ccgtagctgc atgtaaccct agttgtcgac tatgtttgtt
    21541 ttgagactgt tgataatgtg caatagccga tggtaagaat aaaaaaccta catcatgatg
    21601 aacgtctgaa aaatggtaca gggcagattc taataattgt tcttgttgta ttgctgcctc
    21661 tttgaacttt tctcgttttg tcttgtgaaa gagctgccat aaaataccac tccaaaaacc
    21721 attggtccac cagtcgattc ctctgtcctt tattacgtct gtatattttc cgttgtaaga
    21781 aatatatgga attttattcc ccattctgtc taattccgca tctatctttt cagaagctac
    21841 attcaaaatt tttcttgacc attccttcgt ctgttcattc atggatatcc cgccattctt
    21901 ttaattttta tgaatgtgga gttgctccta gaacgaacta gatggttaac attcgttatt
    21961 ttttttactt atttgcagct ccaatgtcat tctccttaaa tttagactgc gcccacacat
    22021 aaatcaagac cattgggatc actgaagttg ctaaaattag cataattgcg ccataattaa
    22081 ttggctcacc tgtactagaa accgtcatat ttttgataat taagggaacc gtatatttat
    22141 ctttatctga aagcatgact aatggaaata gatattgatt ccaacttccc ataaagataa
    22201 gtaaaccact agtaataatc gtaggtgata aaatcggtac agcgattttc gtaaaaatca
    22261 cccactcatt ggcaccatcg attctggcag cctccatcag tgctgtagga aattgactgc
    22321 tatattgtcg catcataaac actgaagttg tactggcaat cgaagggatg atcacacctg
    22381 cataagtatt cgataatcct aaacgactca taatgtaaaa taaaggaatc aatgtggtaa
    22441 aaaaaggcac catcctagat agcataactg tattgaacaa cagtttattc cccataaaac
    22501 gatactttgc taatgcataa cccgccattg tagaactggc tgtagctgaa actgtactga
    22561 ttacagcaat aacaatcgaa ttccaaataa tcaagggata attatatctc gccatgatgg
    22621 cttgccagtt ttttaatagc atatccccaa acttgaaatt gattccgcca cttaaaattt
    22681 cttgattact ttgcgttgaa gctacaatca tatataaata tggcatcaaa aatattaagg
    22741 caccaaacac aataatgacg tacgtaaaaa ttctgctttg tatcttttgt ttagtcattt
    22801 ttatcacctc cgaatttaaa ttgaatagcc gataatataa cagatacaaa caccatcgtc
    22861 caggcaatag cagaggcttt tccaaaatca accatttcaa aacttgtttt atataaattg
    22921 actcctacga ccaaagctga attttctggt ccattggaag tcccaaagaa tatatttggg
    22981 atattgaaat tacccaagcc attgatagtg gctaaaactg tggccattaa aatgattggc
    23041 tttagcatgg ggatcgttaa taaaaagaat tgccgaaatt tcgatgctcc atctatttca
    23101 gcactttcgt atatctcttt agaaatccct tgaagaccag ctaaaaataa aatagtgtta
    23161 tatcctacac taagccaaat catcgtgatg atcattgata ttcgtatcca aaaaggattt
    23221 gttaaccact gtacaccgtt aattcctagc gcattcaaaa atccattgaa taacccagtc
    23281 ggattgaaaa aagtcatgaa tacggaagct acagccaccg ttgatgtaat ggttggaata
    23341 taatagacag ttctaaaaaa tccttttcct tttaactgtg ctgaattcaa taaaacagca
    23401 aagtaaatag ctaatagcat cgttattgga attgatcctg ccatgataat caaaacattc
    23461 gtgtaagatt taataaacaa tggatctcgg aagatagcta gataattgtc aaatccaaca
    23521 aattcaaatg agtagccaat tttcataaaa ctaaaagcaa acgagataat gatgggaatc
    23581 aaaatcgtca caataaagaa aataacaaaa ggtgtgacca atccataagc acttttttta
    23641 tgagacgtat ctgttgcttt tttcttgctg ttctttattg tcttgcttgg taacgaagat
    23701 tgcatcatcg ttaagtcctc ctaatatttt ttaatcacca gttaatttta cgccgtactt
    23761 atgagcaaaa ttttctgctt cttttagata tgaattttca aaatcacctg tttgccaata
    23821 gttatatgca gcggtagtca aataagacgt agcatctgaa taagtggctg gtaaaacagt
    23881 atttgatgta ttttcattga ttttattcaa tagttcatag tatttttgtc ctccttagta
    23941 gtcaaacttt ttctcagctg cttctgtttg ataggccaat gagttagatg tagccacgcc
    24001 cagctccagt gcgttggcta agcattcttc atctgtcagc ataaatgtaa gaaattgttg
    24061 tgccacttgt ggatttttgg catctttagg gacataaaaa cttgatcctc ctgatactgc
    24121 accttttcct ggattttctt ttgaaaaagg aatcagtgga gccatgcgcc atttgtcaga
    24181 tgcatcagga aaattcaaag acatattcgt agcataccag ccgccagcaa caaacattgc
    24241 agattcttga aatgctttct cagcatcttg tgcaccgtag aaatgtacaa tatctgagtc
    24301 gatcatttct ttgattaact ttgcagcatg tttcgcttct ttagtattca gacggatatt
    24361 tccttcttca tcaagcaaag attgattctg ctgggctagc atgttgataa acaattcttg
    24421 gtccccagat tctggcaagg caattccaaa actaccagtt ttttctttta atgcttttgc
    24481 cacctggata tattcttccc aagaactgat ttcttcaaaa tctacaccta tttcagcaaa
    24541 tttttttggt tgataataac aaagtgctgg ggtaaaatca ctagcccaag gaataatcgt
    24601 ttcattactc gtttgttgac gcaaaatatt caatttttga tcataaaact gatcgccata
    24661 tttgtcataa aatccatatt catcagcact ataaaaagca tcttgaaatt tttccagcac
    24721 acctgctgct gaagtgtctg ccatggagat aatatccggc atatcttcgt gtgttaccaa
    24781 ttttggggtg atcatttgta ctgttgctgt tcccgaagct acattcacaa tttttaagtc
    24841 aatatttgct ccagttttgc ggttatagat ttttgcagca ttttcaacat agtttgtcag
    24901 tttgccgttt gcgacatata cttctaatgt agtggctttt tttatcattg gatctgcgat
    24961 gtttgctgtt acttcttcgt tagtttttcc acatgcactt aagctgaata atagggtaat
    25021 gtttagcaaa gctatcacca atttgtatct ttttttcatg tctatccctt ctttcttttc
    25081 aagttcttat ataaaacgct ttcacttatg tatattaacg gaattagtaa cacaaatatt
    25141 tatataaaaa aatattttac ttgtcgtttt ggcttttcat ttttgttata atagtcatta
    25201 attataaata gaaagctttt tatataaaaa gagaaaggaa agtggttctt ttgttaacaa
    25261 acaaccaatc ctccaatctt gtcaaaaaaa cactaaccta ttccatagat acccatcttc
    25321 atttatctgc tgctccctat tttcgactac taataaatga aagcgctaat ttatatgctg
    25381 aatttgcaca taaatctata gaactagata atccttccct tttattcatt gataaacagc
    25441 aagaagtcca aatcttttct actcaaaagg aaacacaaac ttttatcatt atccagtttt
    25501 attttgatga gcaattatta gcacatatta cgggacaatc tttttctata gatcaagaaa
    25561 taacatctat cattactttt ctgaaaaatg aacagttcaa aattgattat ctaaaacata
    25621 gtaagccagc tatttcctat gatcaggaaa tcaattcttt tttcatacta tgccaacaga
    25681 tgagcattca tttgctttcc ttattagtta cttattgtct gcaaaaaatt caattgtctt
    25741 ccttaccaag gcattatcaa caggaacttg ctttattgac aataccttca aatgagaaag
    25801 tcattcctaa aaaaaagcat attctttatt cagatgaatt agttgatcca atcatacgat
    25861 atttagaaga acatctttca gaaaattttt tgcttgaaca attagcccaa atttttttta
    25921 tcagtccagc cagcttaaaa aaacatttta gaaaagtgaa tggaaaaagt gttattagct
    25981 attttaaaga actaaaaatg gaaaaagcaa aacgtctcgt gctagaaaat gaattaacct
    26041 atacagaaat tgcagatata ttaggatttc attcgatcca tcatttttct tcagctttta
    26101 aaaaatatac agggttatcc ccttcaaagt attatctatc cttaaaatag aaaaaaattt
    26161 ttaggtttta tccacaaatt atttctctat attttctgtc tattcaaaat aaacaataat
    26221 tttccggcac aattatttgc tgttttttac cataacatag tacgatcaac gtagaaaagg
    26281 agatgttttt atgaaagttg ttgtcattgg cggtgtagct ggcggtcctt catttgccac
    26341 tcgtttccgt cgattgaatg aagcgcacga aatcattatc tatgaacgcg gagaaaatat
    26401 ttcttatgca agttgtgctt tgccttatta tttaggaggt gtgatcactg accgtgactc
    26461 gctgatcgaa cgtacaccag aaatattgaa aacaaaaaac aacatcgatg tctttactaa
    26521 acacgaagta acagcaatcg atccttctac taagcgatta acagttaaag acctatccac
    26581 aaatgaagaa ataaaaacag attacgataa gttgatcatc tcttctggtg ctagaccaga
    26641 ttatccggat attcccggag tttttgaagc agaaaacggc tttgtcctcc gtagtgtgac
    26701 ggatgcggat cgaatcaaat cgttccttga agaaaaaaat ccacaacatg tcgtcattct
    26761 tggtgcaggt gttatgggtc tggaattagc tgagaatttc aagcatcgcg gcttagacgt
    26821 gactttaatc gatcaattgc cacaagtcgc tttcccttat gatccagaaa ttgctaattt
    26881 agtttatgac aaattgctga aagaaggatt agccgttcat ttagaaacaa gagttactga
    26941 gatccgtgat aaaggtcgag aaatagtatt atcagatggc tccgtccttt ctgctgatat
    27001 gctaattttt gctgttggtg tttctccgaa gaatgaagtg gtgaaagcaa ccggcataca
    27061 attatcagat acaggtcaga tcattgttga tgaccagtta caaaccaatc tgccggacat
    27121 ctatgcgatt ggcgatatta tcgaaacgac tagtgtagtg actggtcagc cgatccaaag
    27181 tatgctttcc agtgcggcca atcgtcaagg acatatgctg gcagatattt taaatggtac
    27241 gcctatgcgc tatcgcggat atattggtgc aggtgtcgca aaaatctttg atcatacagc
    27301 aagttatgcg ggaatgacag aacatgaatt aaaagcatca ggcataacaa attataaaac
    27361 tgtttttatc actccctttg accatgccta tttctatcca gaagctacaa gattaaatct
    27421 aaagctgatt tttgatgcag atagcggtcg tattttaggt ggacaagcat ttggagaaaa
    27481 aggtgtcgat aaacggatgg gagaactttc tgtagcaatc accggaaact tgacagtctt
    27541 tgatttgcct gatttggagt tgccttactc tccaccgtat tctactaccc gtgacccgtt
    27601 gaatatagct ggttatgtcg caatcaatca aatgacgaat atcgtagaaa cgatcaaagc
    27661 aagtgatata cccgaaaacg atttgaaaga agcgttcttt ttagatatac gtgaacctaa
    27721 taaagcacct tctggcagta tctcggcaac aaaaaatatt ccaatgaatg agttacgaga
    27781 tcgaatcaat gaaatcccaa aagataaaaa agtttacatt actttcagaa gaggattgaa
    27841 tacttatact tctgcccgaa tcttggcagg tttgggtatc aaagcgattt tgattgaaga
    27901 ataaaaaaac gaccgcggtg ggtagccgcg gtctaggagt tatttttact tgaggagtaa
    27961 aaatgaaaaa gtatataggg ttgttggttg gtatgtatat agaatagaag ataaatgtga
    28021 agaaattgtg cattatttct tcacatttat taggttatag tttaagaaag tttaaacatt
    28081 gcgcaattta attattttag ctgtttcttt tcataaaaaa acatgttgtg actctgagac
    28141 aaatcatagt ccatagtgat gtctctagag taactacgcc taatattacg aggcatcata
    28201 ttaatctatc agcactagta cctaaactag catccctttc gaatattttt tcttatataa
    28261 taaagtttgc tttcactcct ctaatctcac caaatcgact ggcttgcgtt gtgtgatact
    28321 aggaactgat tggtttagaa cgacttttct attgtaatca tatttttcgc catcctcgtt
    28381 gtcttctaat ccttcaatga atgatcccta atgtgtgtat taaataattt aatttttttg
    28441 ttcctattta tttaaca
//
LOCUS       DAZKLM010000028        28306 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3764
            SAMEA115706913-rid22582563.denovo.028, whole genome shotgun
            sequence.
ACCESSION   DAZKLM010000028 DAZKLM010000000
VERSION     DAZKLM010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706913
            Sequence Read Archive: ERR13196647
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 28306)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28306)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287823.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287823.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,004
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,849
            CDSs (with protein)               :: 2,849
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 41 of 96
            Pseudo Genes (incomplete)         :: 52 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 15 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28306
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706913-rid22582563.denovo.028"
                     /isolate="VRE3764"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(147..335)
                     /gene="rpmB"
                     /locus_tag="ACG842_001129"
     CDS             complement(147..335)
                     /gene="rpmB"
                     /locus_tag="ACG842_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008536053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HFQ0247688.1"
                     /translation="MAKVCYFTGRKTKSGNNRSHAMNSTKRTVKPNLQKVRVMVDGKP
                     KKVWVSTRALKSGKVERV"
     gene            605..967
                     /locus_tag="ACG842_001130"
     CDS             605..967
                     /locus_tag="ACG842_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HFQ0247689.1"
                     /translation="MAVKIQTSAGTIEITNDVIATVVGGAATDVFGVVGMASKNQIKD
                     NITEILRKENYSRGVVVRQEDNGIAVDVYTIISYGTKISEVSRNVQEKVKYNLEALLG
                     VTANSVNVFVQGVRVLPD"
     gene            1019..2701
                     /locus_tag="ACG842_001131"
     CDS             1019..2701
                     /locus_tag="ACG842_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HFQ0247690.1"
                     /translation="MSVTEISASQFQEMVQAGANRLQVNAEYVNSLNVFPVPDGDTGT
                     NMNLSMTSGAKAVVDSTSEKVGELAAMLSKGLLMGARGNSGVILSQLFRGFSKQIPDV
                     VSLNASDLAAAFTHGVETAYKAVMKPVEGTILTVAREAAKAGEKKAKSTDDVVEVMTA
                     VVKGAKRALAKTPDLLPVLKEVGVVDSGGQGLLFVYEGFLSALNGEFQADETYEPSPA
                     EMDDMVNAEHHRSVQGQMATEDIHFGYCTEIMVKIGEGPTVDSTFDYEEFRNYLDGIG
                     DSLLVVNDDEIIKVHVHTENPGEVLNYGQKFGSLVKIKVDNMRLQHETILEHDEEVNE
                     FAKKEIPRIPYGIVAIAAGKGVQELFESLGANYVISGGQTMNPSTEDIVKAIQEVNAD
                     KVIVLPNNKNIFMAADQAAEVADIPVAVVPSRTISQGLTAMLSFNDQASLEENKETMT
                     EMLSSVVSGQVTHAIRDTSIDGVEIKEGDFLGMIDGKILISNPDITATALSTLEQMIN
                     EDTEIVTILAGDEGTVEQANEFAEKLTEKHPDLEIEIHQGDQPVYPYLFSAE"
     gene            2818..4854
                     /gene="recG"
                     /locus_tag="ACG842_001132"
     CDS             2818..4854
                     /gene="recG"
                     /locus_tag="ACG842_001132"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HFQ0247691.1"
                     /translation="MRSLDDPITMLAGVGEKRAASLASLGIQTIEDLLTYYPFRYEDI
                     QERNLNEIQDQEKVTLKGIVVSPPVMNRFGYKKSRLQFRMMQEHDVFNVSFFNQPYLK
                     EKVILSEEIAVYGKWDAKRKALNGMKILGSQTMDDFSPIYHVNKSIRQTTLVDLIKKG
                     FQEYGTLIEENLPNNLVEKYRLLDRASAVRSMHFPKDHEENHQAKRRVVFEEFFLFQM
                     RLQGLKKAEKAETNGLEILYDVQKLKQFTQKLPFELTNAQKRVTNEICRDLRSPQHMQ
                     RLLQGDVGSGKTVVAAIALYATVTAGFQGALMVPTEILAQQHMESLQQLFDPNEVTIA
                     LITGSTKAKERRELLEKLEQGEINVVIGTHALIQDGVEFQNLGLVITDEQHRFGVNQR
                     KVLREKGWRPDVLFMTATPIPRTLAITAYGEMDVSIIDELPAGRIPIETRWVRTPQLD
                     SVLDWTKKELARGHQMYVICPLIEESEALDVKNAVEIYEKLRELFAPKYEVGLLHGKM
                     KNQEKEVIMEAFKDNQMQILVSTTVIEVGVNVPNATVMLIMDADRFGLAQLHQLRGRV
                     GRGSDASYCILVANPKNELGVERMKIMTETTNGFVLSEKDLELRGPGEVFGFRQSGLP
                     QFAIADLVTDGNVLEVAREEAAALWQIKDWQMLPEYQPLAQSLQIENQDNRYFD"
     gene            4903..5904
                     /gene="plsX"
                     /locus_tag="ACG842_001133"
     CDS             4903..5904
                     /gene="plsX"
                     /locus_tag="ACG842_001133"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HFQ0247692.1"
                     /translation="MKIAVDAMGGDHAPQAIVEGVALAQQDFPEVEFLLYGKEAEIKK
                     YLTNEKNITIIHTDEKINSDDEPVKAIRRKKTASMVLAAQAVKNGEADAVFSAGNTGA
                     LLAAGLFIVGRIKNIERPGLMSTLPVVGKEGAGFDMLDLGANAENKPEHLLQYGILGS
                     FYARKVRGIGRPRVALLNNGTEATKGSEVTKKAYELLQNETSLNFIGNVEARELLNGV
                     ADVVVTDGFTGNAVLKSIEGTAMNMMNLLKSAILNEGIKGKMGAMLLKDGLRSLKAEM
                     DYSKHGGAVLFGLKAPVIKTHGATGPDAVRYTIRQIHTMLETDVVGQLVEQFEKSEE"
     gene            6026..6268
                     /gene="acpP"
                     /locus_tag="ACG842_001134"
     CDS             6026..6268
                     /gene="acpP"
                     /locus_tag="ACG842_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFQ0247693.1"
                     /translation="MTREEVFNKVAKIISNHFEIDTDKVTDELNIKDDLKADSISIME
                     FVLELEDEFGTEISDEDAEQIETVGGAVDYISSHLK"
     gene            6536..7540
                     /locus_tag="ACG842_001135"
     CDS             6536..7540
                     /locus_tag="ACG842_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0247694.1"
                     /translation="MSDNQLLEVQNLHTGFRLKDDYYDAVDDVSFTLDKNEILAIVGE
                     SGCGKSTLATTIMGLLDPNNTKITGEILYNDLNLIDLNETLYNKIRGNDIGMIFQDPL
                     SALNPLMRIEDQIKEGLSYHTKMNAEQRQARALELLEQVGIPNPARVGRQYPHELSGG
                     MRQRVIIAIAIACKPPIVIADEPTTALDVTIQAQILDLLKDLQEETKTGIILITHDLG
                     VVAEMADRVAVMYGGQFVEVAGVKELFDHPKHPYTRSLLNSIPQEGNHDAELHVIEGV
                     VPSLKNMPRKGCRFAPRIPWIPASDHEEEPILHEVAPNHLVRCSCYKHFHFRDEKGEV
                     "
     gene            7541..8485
                     /locus_tag="ACG842_001136"
     CDS             7541..8485
                     /locus_tag="ACG842_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0247695.1"
                     /translation="MTELITIKDLKVHYPIRSGFFNRVTDHVYAVDGVDFIIEKGKTY
                     GLVGESGSGKSTTGKAVVGLEKVTAGQIMYEGKDVTKRSNRKKMGYNKDVQMIFQDSM
                     SSLNPKKRVLDIIAEPIRNFERLSDQEEKKKVKGLLDIVGMPEDALYKYPHEFSGGQR
                     QRLGVARAVATNPKLIVADEPVSALDLSVQAQVLNFMKRIQQEYGLSYLFISHDLGVV
                     KHMCDNIAIMYKGRFVEIGTREDIYNDPRHIYTKRLLSAIPQIDVENRESHKENRRRV
                     EQEYIQNQKEYYDATGRVYDLRTITTTHKVALKDGGAS"
     gene            8485..9447
                     /gene="opp4B"
                     /locus_tag="ACG842_001137"
     CDS             8485..9447
                     /gene="opp4B"
                     /locus_tag="ACG842_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease"
                     /protein_id="HFQ0247696.1"
                     /translation="MWKTILRRVLLMIPQVIILSVLIFLLAQAMPGDPFTGLINPNQD
                     PAVIEQMRQAAGLNDPWYVQYFRWIGNALQGDFGQSFLYKRSVADLIGERIINTLYLS
                     ILTVVLTYLIAVPLGMLAGRYQNSILDKIVVIYNFFSFAVPLFIFGLIMLFVFGYRLG
                     WFPTSGSQTLGLQGGFITQWIDRLKYILLPSITQAFLATAVTIQYLRSEVIDSKSLDF
                     VRTARSKGVPTNKVFSRHIFRNAALPMASQMGYEITSLIAGSVVIEKIFGYPGVGKLF
                     IDSIGQRDYTVITSLVLILGIATLVGTLLSDIIMSIVDPRIRIQ"
     gene            9474..10388
                     /locus_tag="ACG842_001138"
     CDS             9474..10388
                     /locus_tag="ACG842_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0247697.1"
                     /translation="MSEKNNTAVQESIPPMGIRMIAREFKKDKIAIFSLGLLVVLLLV
                     IFIGAMLTDQDKVMYVSIFDKYAAPGTVSTQGQKFILGADEGGRDVLGQLIIGARNSV
                     TIGFAITILTSIIGVGLGIISGYYGGIIDNILMRIVDFIMILPIMLIIIVFVSIISRY
                     NVWSFIFIMSAFYWVAKARLFRSKTLSEARRDYVSASKTMGTNDFKIMFREIMPNLSS
                     LIITNLTMNFAANIGIETTLTFLGFGLPANVPSLGTLIGYASNGEVLVNRQWIWLPAS
                     ILILVLMLSINYVGQAFKRSADARQRLG"
     gene            10414..12195
                     /locus_tag="ACG842_001139"
     CDS             10414..12195
                     /locus_tag="ACG842_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HFQ0247698.1"
                     /translation="MKKTIFGFAALMSAVVLAACGGGGTDTSGNNSGGNGNNKEELTF
                     PIATKNNDDAIEDGELEAAVATDSQFKGLFQWEFYQDSYDAAFMAPSHEPLFANDDDF
                     RITDDGAATLDLDEDTKKATIKVKDNVKWSDGEDVTADDVIFPYEIIGNKDYTGIRYD
                     DTFRNIVGMEEYHDGSADTISGIKKIDDKTVEIEYKEVNPSMLQSGGGIWSYAAPKHT
                     LEGVAIKDMESSDQVRKNPVTFGPYYMSNIVAGESVEYLPNEHYWNGTPKLKKITMKS
                     VPMASATEALNSKLYDMIFQMPTDTYDTYKEIAGYTNLGREQTSYTYLGFKLGKWNAD
                     TKSVEYNPDSKMADKSLRQAMGYALDNNAVGERFYAGLRSNATSLIPPVFQSYHDDEM
                     KGFTQDLDKAKKLLDDAGYKDVDDDGLREDKDGNKLTINFASMSGGETAQPLADYYIQ
                     QWKEIGLDVQYTTGRLIEFNSFYDKLENDDPEIDVYQAAWNTGTDPNPSGLWGPNAAF
                     NYTRYESEENTKLINDISSSKAFDEEYQKEAYQKWQQYAFEEAFAIPTLFRNEVLPVN
                     DRVKDWDWSYDVPNPWANVSVTADSRS"
     gene            12543..13229
                     /gene="rnc"
                     /locus_tag="ACG842_001140"
     CDS             12543..13229
                     /gene="rnc"
                     /locus_tag="ACG842_001140"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HFQ0247699.1"
                     /translation="MDNQLTKELKENYNIVFHDLNLLEQAFTHSSYVNEHRNLQLSDN
                     ERLEFLGDAVLELMVSQYLFRLYPHLPEGKLTKTRAAIVREDSLSKFAKECHFDQYVL
                     LGKGEENSGGRTRPALLCDLFEAFLGALYLDQGFDTTHAFIEKVIFPKVKAGAFSHEM
                     DHKTKLQEVLQKSGDVSIEYRLINEEGPAHERVFWIEVYVDDQLIGTGQGKSKKLAEQ
                     AAAENALAAL"
     gene            13249..16830
                     /gene="smc"
                     /locus_tag="ACG842_001141"
     CDS             13249..16830
                     /gene="smc"
                     /locus_tag="ACG842_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HFQ0247700.1"
                     /translation="MYLKRIEIAGFKSFADKTVIDFENSVTAVVGPNGSGKSNITEAI
                     RWVLGEQSAKSLRGGKMPDIIFAGSDTRKQLNIAEVTVILDNSDHYLPLEYNEISVTR
                     RYRRTGESEFFINKQSCRLKDIQELFLDSGLGKESFSIISQGKVEAIFSSKPEDRRGI
                     FEEAAGVLKYKQRKKKAEQKLFETEDNLSRVQDIIHELEEQLTPLAAQSEAAKEFLRL
                     KETLTQTDVSLMVAEIKTAKKDWDNKQAQLAKFNLELGKLSESIQEQESILAKQRKEN
                     AQADRLIEKNQQVLLDLSEKLKQTEGQKDVLQERTKHTQKSSQEYQTSLAEAQKKVKH
                     FEKLQESLMKAAAEKETEIQKAEANLIKTQQELEKYQKSTKELLAELRDQYVDLMQEQ
                     AAVGNELKYLERQYIQETAKSKQTLAKQSEVEASVDRLILQKEELTQKQAQLKSSLTE
                     TKEKLEMIQQNGKKFQEALAKEQPKMYQLMNQVQQLRARKKSLQEIQENYFGFYQGVR
                     LVLQHKQQLSGIVGAVAELIDVPADFTLAIEAALGGAAQHVIVENEKDARQAITYLKQ
                     QRGGRATFLPLTTIKPRQLPAHILTQAAAVEGFIGIASEQVSYPDQIQTVVQNLLGTI
                     LLAKDLTSANAIAQTIRYQYRVVSLEGDVMNAGGSMTGGANKRGNQGSLFVQNQELKQ
                     LTSEFEEADKQLQAQEKKVQELQQETARLAEEQEVLRTRGEQLRFEEQEATNQLQNII
                     NELERFEKEKQISTFETRELQQFIEDYEKQTNELKDKQTDLESQRQKIDEEIKSLSQE
                     SDQMEARRAQVQSQKAQEQADLAVLKEQFNHLQIQLRGARVQKAEATERQEAIEKQLA
                     TLTADFSDHEVTEESLEKQINELSAQRETLKAELAKAKEQRDRRQKEIDKLEAVLAER
                     NREQKARLSEQSKLEVQKDRAEMILDNHLTYLQSEYQLTFEKASQDYQETTDIEDSRT
                     KVSSLKEQIEKLGPVNLNSIEQYEQVSERHTFLATQRDDLLAAKNQLFETMDEMDDEV
                     RTRFKEVFEAIRQEFKIVFPNMFGGGKAELVLTEPTDLLKTGIEIEVQPPGKKLQSLS
                     LLSGGERALTAIALLFSIIRVCPVPFCILDEVEAALDEANVKRFGRYLSDFQDDTQFI
                     VVTHRKGTMVAADVLYGVTMQESGVSKIVSVRMEDINEEAKINETKV"
     gene            16839..17648
                     /locus_tag="ACG842_001142"
     CDS             16839..17648
                     /locus_tag="ACG842_001142"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFQ0247701.1"
                     /translation="MIKLVAIDLDGTLLNEQKLISDENKQALAQAKQQGVKIVLCTGR
                     PLAAMAHYLQELGLVDEGDYSITFNGGLVQKNDTGEIMEKKVMEVSDIQRLYKLAQQL
                     DLPLDVLSDNVVLQLPSAPRKQSLYNVLNKLLQFQPADISDITDEFVLNKAVIAYDQE
                     ELDPKIKEIPAEFHEMYEIIKTRSMLLEFMPKGVTKAYGISLLAKDLGLKQEEIMAIG
                     DEENDLPMIQYAGLGVAMENAVPFVKEAADFVTASNEENGVAKVIQKFVLE"
     gene            17659..18657
                     /gene="ftsY"
                     /locus_tag="ACG842_001143"
     CDS             17659..18657
                     /gene="ftsY"
                     /locus_tag="ACG842_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HFQ0247702.1"
                     /translation="MGFFDKIKRAFTGEEKTEEVEVQEKYDKGLEKTRKTFGERMNEL
                     FANFRTVDEDFFEELEETLIGADVGFETAIRITEALRQEVKLRNAKKSAEVQNAIIEK
                     LVDLYEAEGINEVNELNIQEDGLTVILFVGVNGVGKTTSIGKLAHQFKNEGKKVLMAA
                     ADTFRAGAIDQLVVWGERAGVEVVRGNAGGDPAAVVFDALERAKTEQADVLLVDTAGR
                     LQNKVNLMNELEKIKRVIQRELPEAPQETLLVLDATTGQNAMVQAKQFKETTDVTGLV
                     LTKLDGTAKGGIVLAIRNELHLPVKLVGLGEGIDDLEPFDPNDFVVGLFKGLLKEE"
     gene            18844..21111
                     /gene="gshAB"
                     /locus_tag="ACG842_001144"
     CDS             18844..21111
                     /gene="gshAB"
                     /locus_tag="ACG842_001144"
                     /EC_number="6.3.2.2"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate--cysteine ligase
                     GshA/glutathione synthetase GshB"
                     /protein_id="HFQ0247703.1"
                     /translation="MMNFKQLLLHVNARPFIDQARFGIEREGQRVDLAGNLAKTDHPA
                     IFGDRSYHPYIQTDFSETQTEMITPVTDSIPELFQYLAAVYDVTARSIPKEEMIWPLS
                     MPPALPEKDEEIIIAKLKNFEDVLYRRYLAKEYGKRKQMVSGIHFNFEFGDELLRTLF
                     SHQEEFQDFSEFKTELYLKTARNFMRYRWMITYLFGASPMSEKNYFLDESHPQEPVRS
                     IRNSALGYTNHPNVKVSYASMKQYLADIERMIEEGKLSEEKEFYTPLRFRGGKKVADL
                     ATTGVRYIELRNIDLNPYARLGINPEQVRFLQLFLMYMLWTEEKEDCDQWVAEGTTRN
                     NKVALEQPSDQTEFHQEGREILEGMKQMLVELDWLDSLYLVEEALTQMDHPEQTLAAK
                     LYQEAQLSSQQEVAVALGHQYYKESHERPYQLAGFREMELSTQIFMFDAIQKGVQVKV
                     LDESDQFLRLQFQDHVEYVKNANMTSKDSYIVPLIMENKTVTKKVLKEAGFRVPGGAE
                     FSSMEEAVKAYPRFAEQAFVIKPKSTNYGLGITIFKEGASLEDYQAGLAIAFREDSSV
                     LVEEFMPGTEYRFFVIDGEVQAIMLRVPANVIGDSIRTVKELVEEKNSDPLRGTNHRA
                     PLELIQLGELEQLMLKEQGLTIESVPQANQIVYLRENSNISTGGDSIDMTDEFSEAYK
                     KIAVSAVEALGAKISGIDLIIPDKEIDPTTDKKAYGIIEANFNPAMHMHVYPFAGKGR
                     RLTMNVLKLLYPEVF"
     gene            complement(21200..21652)
                     /locus_tag="ACG842_001145"
     CDS             complement(21200..21652)
                     /locus_tag="ACG842_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueI family protein"
                     /protein_id="HFQ0247704.1"
                     /translation="MAQDDVKSHLDKGLYGTPLVNPEEQHKYLGTFRERCYLSMTVAE
                     MIQPKNKENFLKELKKHPDASILLNGALPLSVQNQYIQLATKNNSRFTVVNDYVTANP
                     EEFGLLLTAKEAVNEPVIDIEKKYPEENSSPAEKEKPKKKKSFWDRLF"
     gene            21802..22752
                     /locus_tag="ACG842_001146"
     CDS             21802..22752
                     /locus_tag="ACG842_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HFQ0247705.1"
                     /translation="MRYGRLVALLVILSSISLFIGVSNVQLSELLQLDGQSLQILLVT
                     RLPRTICLILVGATSSICGLIMQHLTQNKFVSPTTAGTMDSARLGILVAMIFLPGASL
                     IVRSLTAFCFAFAGTLLFLSLTRLFLQKNQLMLPLIGVMLGNIIGSVATFFAYQFQLV
                     QNMTSWLQGNFATVIRGNYELLYAVVPLLLLVWLFAYRFTIIGLGESFAVNLGIDYQK
                     MQFLGIGLVSLASAVMLIMVGSIPFIGVIIPNLVSRIYGDQVHKTIGVTALAGSLFLV
                     FCDLVARVLIFPYEIPVSVIVGIIGGAIFLYLLIRGRRYA"
     gene            22745..23704
                     /locus_tag="ACG842_001147"
     CDS             22745..23704
                     /locus_tag="ACG842_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HFQ0247706.1"
                     /translation="MLKKKPSIFIGMLVLVLFVTGMYLTIHTYGNWHFACALRSKKIF
                     AFILVSLAVSSATISFQTITHNQFLTPSVLGLESLYVLVQTVLFFFIGGVTMLQQTGT
                     VFFLLNVGLMVGLSLLLFFFLLKKMRANLFLLLMSGMILGTLFGSVSTFLQVIMDPNE
                     YDLLQGRLFASFGNIQSQYLLPASLIIIGCWYLLFRYRHVLDVFHLGNEQAVNLGIDT
                     DRFEFLILCCVSLLTGTSTALVGPVTFLGFIVANISYRVFSTYRHGQLFIGSFLVAML
                     FLTGGQLAVEQIFGWNTTVSVVVEFVGGIYFVTKLIRERKSIL"
     gene            23701..24456
                     /locus_tag="ACG842_001148"
     CDS             23701..24456
                     /locus_tag="ACG842_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0247707.1"
                     /translation="MIDLQKVNKLYHGKSVVKDVQLTIPEKKLTAFIGPNGAGKSTVL
                     SMISRLIPKDTGEIYLDHNEVKAWKSNELAKKLSILRQSNEINVQITVKELVEFGRFP
                     YTKGKLTTKDEEIVQESLEHIGLEEIADQSIDTLSGGQLQRAYIAMVLAQDTDYILLD
                     EPLNNLDMNFAVQIMQILKNLVQEIGKTIVIVLHDINFAASYADEIVAMKDGQLFTQG
                     TTQEMIKKDVLDELYEMDIRICEIEGRRFCLYF"
     gene            24479..25432
                     /locus_tag="ACG842_001149"
     CDS             24479..25432
                     /locus_tag="ACG842_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HFQ0247708.1"
                     /translation="MKKLVVGILILASFGLAACGNSNNTSQADTKKSSTQTETTLTIT
                     DSNGDQIEVPNNPEKVVVFDNGSLDTMDALGVGDKVVGAATSSLPEYLSSYKKVESAG
                     GIKEPDLEKINQLQPDLIIISGRQRDFQKDLSQIAPTIFLSLDAKNPWESFQQNVTAL
                     GEIFGKQEEAKTQLEELSSAIDQTKKKAEATDKKALVTLVNEGQLSAYGSGSRFGFIH
                     DLFGFEQADDQIEASTHGQSVSYEYVLEKNPDILFVVDRTKAIGGDDSKDDISANELV
                     AQTNAGKNQQIISLEPDVWYLSGGGLESMKLMIEDVNQAFK"
     gene            complement(26076..26372)
                     /locus_tag="ACG842_001150"
     CDS             complement(26076..26372)
                     /locus_tag="ACG842_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanyl-tRNA synthetase subunit alpha"
                     /protein_id="HFQ0247709.1"
                     /translation="MKEYIKNIYFIEETQNIEGSYIEVKTLFVNEDKTKALDIYKKLA
                     SKKTNSFGLILSEYKIKAEESYFYQLLKRWSKLPADFYRKMQIINYQPLAETHA"
     gene            26727..27083
                     /locus_tag="ACG842_001151"
     CDS             26727..27083
                     /locus_tag="ACG842_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HFQ0247710.1"
                     /translation="MKLEVTKEAQEVLKNKLEADDQLLLDIENGDGPFAESQVSCQLD
                     TAFRLIIVKKDTQTDLSLYSVVADTPVGPIKIKDSAILYMDDPSTLALEPTYNSLQLK
                     GPSGLRKGNLQVVRKD"
     gene            27093..27992
                     /locus_tag="ACG842_001152"
     CDS             27093..27992
                     /locus_tag="ACG842_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFQ0247711.1"
                     /translation="MGKLAIISDLHVDINKFGESELMQLWQVLQKHKITRLHLAGDTA
                     NKVDRCLAVVEFFVNKGLSTTFHFGNHELADVPNEQMMDHFPDPHFLNERYIALNEQT
                     VLLGMNGWYDYQFSELKDYEAIVRMKRLYWYDRFIQRGKTDLEVNAEMLAAAKNLLDQ
                     LEAKKLEVILSTHFVPKRAFIMYQSAPYERWNKLNAFLGSESFGELLDRYSNIKQVVF
                     GHTHRRFEDKSIGHTIYSCRPFGYYYEWQLTRDFVLKEQLLDSYDPMKLRVLLRAHHP
                     AFQAYQTQHLQEEFEQAMTIVPY"
BASE COUNT         9393 a         4969 c         6108 g         7836 t
ORIGIN      
        1 aataaaattt agaataaata acttcataat ttgttcgtat aacaaaaata aacgcaaaaa
       61 aagggtagga atcaaaagtc ccacccttaa aaatttttat taaataaaaa accgttcact
      121 atctgaacgg tccaagtatc attcaattaa acgcgttcaa cttttcctga tttcaaagca
      181 cgagttgaca cccaaacttt tttaggtttt ccgtcgacca taacgcgaac tttttgtaag
      241 ttaggtttaa cagtacgttt tgttgagttc atagcgtgtg aacggttgtt tccacttttt
      301 gtcttgcgac ctgtaaagta acaaactttt gccatggtaa tttcctcctt tgccttgatt
      361 tggagcaata gaataattca gctcatacca gactaacata tcatatagaa tgggaatttg
      421 caagtacatt ttcatagata caattgacaa ttgagaagaa taattgtaaa ttaaacaagg
      481 ttatacctga aattttgcaa ggtttttgag tactttgggt aactgccttt tatttcaagt
      541 tgcgctatga taaaatatga tagaagaaaa atgggagttt tttacccgtt aaggaggctc
      601 attcatggct gtaaaaattc aaacgtcagc tggaaccatt gaaattacca atgatgtaat
      661 tgcaacagtt gttggtggcg ccgcgacaga tgtgttcggt gttgtaggaa tggccagcaa
      721 aaatcaaatt aaagataata ttactgagat tttgcgtaaa gaaaattact caagaggagt
      781 agttgtccgc caagaagata atggtattgc tgtagatgtt tatacgatta tcagctatgg
      841 tacgaaaatc tctgaagttt cccgcaacgt ccaagaaaaa gtgaaataca atctagaggc
      901 attattaggc gtaactgcca attcagttaa tgtctttgta caaggtgttc gtgtactgcc
      961 agactaggca ggagactggc tggtaactca gcagtcgatc tgaaggagga ttatctaggt
     1021 gagtgtaaca gaaataagtg caagccagtt ccaggaaatg gtccaggcag gtgcgaatcg
     1081 tctgcaagta aatgcagaat acgtcaattc attaaatgta ttccctgtgc cagatggcga
     1141 tacgggaaca aatatgaatc tatctatgac tagtggagct aaggcagtag tagattcaac
     1201 ctcagaaaaa gtaggagaat tagcagcaat gctttcaaaa ggattgctga tgggtgcgcg
     1261 cggaaattcc ggcgttattt tgtctcaatt gtttcgtgga ttttctaagc aaattccaga
     1321 tgttgtctca ttaaatgctt ctgatcttgc tgcagccttc actcatggag ttgaaactgc
     1381 gtataaagca gtaatgaaac cagtagaagg aacgattttg acagtagcta gagaagcagc
     1441 gaaagctgga gaaaagaaag caaaaagtac tgatgatgtc gttgaagtaa tgacagctgt
     1501 tgtaaaagga gcaaagcgtg ctttagctaa aactccagac ttgcttccag tcctaaaaga
     1561 agtcggtgtc gtagacagtg gagggcaagg actgctgttt gtttatgagg gctttttaag
     1621 tgcattgaat ggagaattcc aagcggatga aacatatgaa ccttctccag cagaaatgga
     1681 tgatatggtc aatgcagaac atcatcgcag tgtccaaggt caaatggcga cagaagatat
     1741 ccattttggt tactgtacag aaatcatggt aaaaatcgga gaaggaccta cagtcgatag
     1801 tacattcgat tacgaagaat tccgtaacta tttagatggt ataggagatt ctttacttgt
     1861 ggtcaatgat gacgagatca tcaaagtcca cgtgcataca gaaaacccag gagaagtctt
     1921 gaattatgga caaaaattcg gttcattagt caaaatcaaa gtcgacaaca tgcgcttgca
     1981 gcatgagacg attttggaac acgatgaaga agtgaacgag tttgcgaaaa aagaaatacc
     2041 acgtatcccg tatggtatcg tagcgatcgc tgctggtaaa ggcgtccaag agctgtttga
     2101 aagtttaggc gctaattatg ttatcagtgg tggacaaaca atgaatccaa gtacggaaga
     2161 cattgttaaa gcgattcaag aagtaaacgc agataaagtg attgtcttgc caaacaataa
     2221 gaacatcttc atggcagctg atcaagcagc agaagtagca gatatcccag tagcagttgt
     2281 tccttcaaga acgatttccc aaggattgac agctatgctt tcattcaatg atcaagcttc
     2341 tttagaagaa aacaaagaaa cgatgacaga aatgttgtct agcgttgtga gcggtcaagt
     2401 cactcacgcg atcagagata cctcaattga cggtgtagag atcaaagaag gagacttttt
     2461 gggcatgatc gatggaaaaa tcctgatctc taacccagat atcacagcaa cagcgttaag
     2521 tacactagaa caaatgatca atgaggacac agagatcgta acgatcttgg caggtgatga
     2581 aggaacagtt gaacaagcaa atgaatttgc tgagaaacta acagaaaaac atcctgattt
     2641 ggaaatcgag atccaccaag gagatcagcc tgtctatcct tatctatttt ctgctgaata
     2701 atttctgtgt cagaatatct ttctaacaca gtattcattc ataaaaggcc ttcggttagt
     2761 gatcaggtat gatcgctgac caagtccttt ttttgttaaa ggagggagga aaaatttatg
     2821 cgttctttag atgatccaat cactatgctg gcaggagtcg gagaaaaaag agcagctagt
     2881 cttgccagct tgggcatcca aacgattgaa gacttactga cttattatcc ttttcgctac
     2941 gaagatattc aggaaagaaa tctaaacgag atccaggatc aagaaaaggt tactttgaaa
     3001 ggaatcgtag tctcgcctcc tgtgatgaat cgatttggtt ataaaaaaag ccgattgcaa
     3061 tttcgtatga tgcaagaaca tgatgtgttc aacgtttctt tttttaatca accttatcta
     3121 aaagaaaaag tcattttatc ggaggaaatc gccgtatacg gtaaatggga tgcaaagcgt
     3181 aaagccttga acgggatgaa gattttaggt tctcaaacaa tggatgactt ttcgccgata
     3241 tatcacgtca acaaatcaat tcgtcaaacg acattagttg atctaatcaa aaaagggttt
     3301 caagaatacg gcacattgat tgaagaaaat ctgccaaaca accttgtgga gaaatatcga
     3361 ctgcttgacc gagcttctgc tgtaagaagt atgcattttc caaaagacca cgaagaaaac
     3421 catcaagcca aacgacgtgt cgtttttgaa gagttctttt tatttcaaat gcgtcttcaa
     3481 ggattgaaaa aagcagaaaa agcagaaacg aatggtttgg agatccttta tgatgtccaa
     3541 aaattgaaac aatttactca aaagctacct tttgaactga caaatgcaca aaaacgtgta
     3601 acgaatgaaa tctgtcggga tcttcgcagt ccgcagcata tgcagcgact tttgcaggga
     3661 gatgtaggta gcggaaaaac agtggttgcc gcgattgcgt tatacgctac cgttacagca
     3721 ggtttccaag gtgcgttgat ggtgcctaca gaaatcttgg cacaacaaca tatggaaagc
     3781 ttgcaacagc tgtttgatcc caatgaagta accatcgcat tgattacggg gtcaacgaaa
     3841 gcaaaagaac gacgtgagct ccttgaaaaa ctagagcaag gggaaattaa cgtcgtgatc
     3901 ggcacacatg cattgatcca agatggagtc gaatttcaga atttaggtct agttattacg
     3961 gatgagcagc accgttttgg cgtgaaccag cggaaagtac ttcgtgaaaa aggctggcga
     4021 ccagatgtct tatttatgac agcgactccg attcctagaa cattagctat tacagcatat
     4081 ggtgagatgg atgtatctat cattgatgaa cttccagctg gacgtatccc gatcgaaaca
     4141 cgctgggtaa gaacacctca attggacagt gtactggatt ggacgaaaaa agaactggca
     4201 agaggacatc aaatgtatgt catctgtccg ctgattgaag aatcagaagc attagatgta
     4261 aaaaatgcag tggaaatcta tgaaaaactt cgtgaactat ttgcaccaaa atatgaagtt
     4321 ggcttgttac atggtaaaat gaagaatcag gaaaaagaag taatcatgga agccttcaaa
     4381 gacaaccaga tgcagatatt agtttcgact actgtaattg aagtaggggt aaacgtgccg
     4441 aacgcgaccg tcatgctgat catggatgcg gatcgatttg gattggcaca gctgcaccaa
     4501 cttcgtggac gtgttggacg cggaagtgat gcttcctact gtatcttagt tgctaatcct
     4561 aaaaatgaac taggtgtgga acggatgaaa atcatgacag agaccaccaa tggttttgta
     4621 ctaagcgaaa aagatctgga attacgtggt cctggtgaag tatttggctt ccgtcaatct
     4681 ggtctgcctc aatttgcgat tgctgacttg gtgacggatg gaaatgtttt ggaggtagcc
     4741 agagaagagg ctgcagccct ttggcaaata aaggattggc agatgcttcc tgagtatcag
     4801 cctttagcgc aaagcctcca aatagaaaat caggataatc gttatttcga ttagcagact
     4861 gtgctatact ttatactgat gacggaaaaa ggagtggaaa caatgaaaat tgccgtagat
     4921 gcaatgggcg gagatcacgc acctcaagca attgtagaag gcgtggcttt agcgcagcaa
     4981 gattttccag aagtagaatt cttactttac ggaaaagaag cagaaatcaa gaaatactta
     5041 acaaatgaaa aaaacatcac gattatccat acagacgaaa aaatcaatag tgatgatgag
     5101 ccagtaaaag caattcgacg aaaaaagact gcatcaatgg tccttgctgc acaagcagtg
     5161 aaaaacggtg aagcagatgc tgtattttca gcagggaata caggcgcgct actagcagca
     5221 gggttgttca tcgtaggacg tatcaaaaat atcgaacgcc ctggcttgat gtctaccttg
     5281 ccagtagtag ggaaagaagg tgccggtttc gatatgctgg atcttggcgc taacgcagaa
     5341 aacaaaccag aacatttgtt gcaatacggt attttaggtt ctttttatgc acgtaaagtc
     5401 cgaggtatcg gacgtcctcg tgtcgcactc ttaaacaacg gaacagaagc aacaaaaggt
     5461 agtgaagtga cgaaaaaagc ctatgaactc cttcagaatg aaacaagctt gaattttatt
     5521 ggaaacgtag aagctcgtga gctactcaat ggtgtggctg atgtagtggt cacagatgga
     5581 tttacaggca acgctgtttt gaaatctatt gaaggaacag caatgaatat gatgaatcta
     5641 ttaaaatctg ctattctaaa tgaagggatc aaaggaaaaa tgggcgctat gcttttaaaa
     5701 gacggcttac gttctttgaa agcagaaatg gattattcca aacatggcgg tgccgttttg
     5761 tttggcttga aagcaccagt cattaaaact catggagcaa caggccccga tgctgttcgc
     5821 tatacgatcc gccaaatcca cacaatgctt gagacagatg tagttggaca attagtcgaa
     5881 caatttgaaa aaagcgaaga atagtatttg gatatctgaa gcgatccgtt ccattgaaaa
     5941 aaaggtggac attcctgaaa aatgccagta aaattagcca tggtgtaggt aattgacaag
     6001 tgtatgattc ttggaggtga aaaagttgac tcgcgaagaa gtatttaaca aagtagcgaa
     6061 aattatttca aatcattttg agatagatac cgataaagtg acggatgaat tgaacatcaa
     6121 agatgattta aaagctgatt caattagtat tatggaattc gttttagagc ttgaagatga
     6181 attcggtacg gaaatttcag atgaagacgc tgaacaaatt gaaacagtcg gtggcgcagt
     6241 agattatatc agcagccatc ttaaataatt tcaaataaaa gcaaagaagg ataaaaagct
     6301 gcatgcagct ttttatcctt ctttttttaa tctttttgac acgtaaaagt catttttctg
     6361 ataattctgg cgataaacag aaattatgtt gcaaagtaaa gcggttttct atataattat
     6421 taataattca ttacacaaag ataagaataa cctttgttat aaaacgattt attctgtgta
     6481 atgtcctttt ttattaagaa aaacttccat ttaggaagca gagaggggta ttcgattgtc
     6541 cgataaccaa ttattagaag ttcagaacct acatacagga ttccgactca aagatgacta
     6601 ttacgatgca gtagatgacg tttcatttac tttagacaaa aacgagattt tagcaatcgt
     6661 tggggaatct gggtgcggga aaagtacctt ggctactacg attatgggat tacttgatcc
     6721 taataacaca aaaattacag gtgagatttt atataacgat ctgaatttaa tcgacctgaa
     6781 cgaaacgctc tataataaaa tccgcggaaa tgatatcgga atgattttcc aagatccgtt
     6841 gtctgcatta aatcctctca tgagaatcga agaccagatc aaagaaggac tttcctatca
     6901 cacaaagatg aatgcagaac agcgtcaagc tcgtgcacta gaattgctag aacaagtagg
     6961 gattcctaat cctgcgagag ttggacgcca atatccccat gaactatcag gcggtatgag
     7021 acaacgtgtc atcatcgcaa tcgcaatcgc ctgcaaacct ccaatcgtca ttgcagatga
     7081 accgacgaca gccctagatg taaccatcca agcacagatt cttgatttac tgaaagacct
     7141 tcaggaagaa acaaaaacag ggatcatctt gatcactcat gacttaggag tcgtagcaga
     7201 aatggctgat cgagtggcag tgatgtatgg cggacagttc gttgaagtag caggtgtaaa
     7261 agaactgttt gatcatccta agcatcctta tacacgctct ttactgaact ctataccaca
     7321 agaaggaaat catgatgcag aactccatgt gatcgaaggt gtcgttcctt ctctgaaaaa
     7381 catgcctcgt aaaggttgtc ggtttgcacc acgtattcca tggataccag caagcgatca
     7441 tgaagaagag cctatccttc atgaagtcgc accgaaccac ttggtacgtt gctcctgcta
     7501 taagcatttc cactttagag acgaaaaagg agaggtgtag atgacagaat taatcacaat
     7561 caaagacctg aaagttcatt accctattcg cagtggattc ttcaatcgag taacggatca
     7621 tgtatatgct gtagatggtg tggattttat tatcgaaaaa ggaaagacat atggtttagt
     7681 gggagagtct ggctctggaa aatctactac gggaaaagcc gttgtgggat tagaaaaggt
     7741 aacagccgga caaatcatgt atgaaggaaa agatgtaacg aagcgaagca accgcaaaaa
     7801 aatgggttat aacaaggatg tgcagatgat cttccaagat tcaatgtcca gtttgaatcc
     7861 taaaaagcga gtgttggata tcattgctga acccatccga aattttgaac ggttaagcga
     7921 tcaagaagaa aagaaaaagg taaaaggact gctagatatc gttgggatgc cggaagatgc
     7981 gctatataaa tatcctcatg aattttcagg cggacaacgt caacgtctag gagtagcccg
     8041 tgctgttgct accaatccaa aactgatcgt agccgatgaa cctgtatctg ctctggatct
     8101 atctgttcaa gcgcaagtat tgaatttcat gaaacgaatc cagcaagaat acggcctcag
     8161 ctatttattc atttcacatg atttaggtgt agtaaaacac atgtgcgaca atatcgcaat
     8221 tatgtacaaa ggtcgatttg tagaaatcgg tacaagagaa gacatttata atgacccgcg
     8281 acatatctat acgaaacgtc tgctttcagc tattccgcaa atcgatgtag aaaataggga
     8341 aagccacaaa gaaaatcgac gaagagtaga acaagagtat atccaaaacc aaaaagaata
     8401 ctatgatgcg actggtcgag tctacgattt gcgtacgatc acaactacac acaaagtggc
     8461 gttgaaagat ggaggtgcaa gctaatgtgg aaaacgattc tgcgacgtgt tttattgatg
     8521 atcccgcaag ttattatttt aagtgttctg atcttcttat tagcccaagc gatgccgggg
     8581 gatccattta ccggtttgat caatccaaat caggatccgg cagtgatcga acagatgcga
     8641 caagcagctg gcttgaatga cccatggtat gtccagtact tccgttggat cggaaatgct
     8701 ttgcaaggcg attttggtca aagtttctta tacaaacgct ctgttgctga cttgatcggc
     8761 gaacggatca tcaacacttt atatctatcg atcttgacag ttgtccttac ttatttgatt
     8821 gctgtgcctc tggggatgct tgctggccgg tatcaaaatt caatcttgga taagattgtc
     8881 gtaatctaca acttctttag ttttgctgtt cctttattta tcttcggctt gatcatgcta
     8941 tttgtttttg gttatcgttt aggctggttc ccaacaagtg gatcacaaac actaggtttg
     9001 caaggtggat ttatcactca atggatagac cgtttgaaat atattctgtt accatcaatt
     9061 acccaagcat ttttggcgac tgccgtcaca atccaatatc tgcgaagtga agtaatcgat
     9121 tcgaaatcgc tcgattttgt cagaacagcc cgttccaaag gggtaccgac gaataaagta
     9181 ttcagtcgac atattttccg aaatgcagcg ttgccgatgg cttctcaaat ggggtatgaa
     9241 atcacttcgt tgattgctgg ttctgtagtt atcgaaaaga tttttggtta tccgggagtc
     9301 ggaaaactat ttattgattc catcggccaa cgggactata ccgtgatcac atccttagta
     9361 ttgattttag gaatcgcgac gctagtgggg acattacttt cagatatcat catgagtatc
     9421 gttgacccgc gtattcggat tcaataaaac caatcagaaa ggagaatgga tcgatgagtg
     9481 aaaaaaacaa tacagcagtt caagaaagta ttcctcctat ggggatacga atgattgctc
     9541 gagaatttaa aaaagataaa attgctattt tttcattagg tttgctagtg gttttattat
     9601 tagtgatctt tatcggtgcg atgctgaccg atcaagacaa agtgatgtat gttagtatct
     9661 tcgacaaata cgctgcacca ggaacagttt cgacacaagg acaaaagttc attctgggag
     9721 cagatgaagg cggacgtgat gtattaggac aattgatcat aggtgctaga aactctgtta
     9781 cgatcggatt tgcgatcacg atccttactt cgatcatcgg ggttggatta gggatcatct
     9841 ctggctacta cggagggatc attgacaaca tcttgatgcg gatcgttgat tttatcatga
     9901 ttctaccgat catgctgatc attatcgtct tcgtatcgat tatttccaga tacaatgtct
     9961 ggtcctttat attcatcatg agtgcatttt attgggtagc taaagcccga ttattccgaa
    10021 gcaagacatt atctgaagca agaagagatt atgtcagtgc ctcaaaaaca atgggaacca
    10081 atgattttaa aatcatgttc cgcgaaatca tgccgaatct aagttcatta atcattacta
    10141 atttgacgat gaactttgcg gcaaacatcg gtatcgagac aactttgact ttcttaggtt
    10201 tcggcttgcc ggcaaatgta cctagtttag gtacattgat cggttatgca agtaacggag
    10261 aagtcttagt gaatcggcaa tggatctggt tacccgcatc aatccttatt ttagttttga
    10321 tgttgagtat caattatgtt ggacaagcat tcaagcgttc tgcagatgcg cgccaacggt
    10381 taggctagta tagaagcgaa gggggaaata ggaatgaaga aaacgatttt tggatttgca
    10441 gcgctgatgt ctgctgtcgt tcttgcagca tgcgggggcg gaggtacaga tacctctgga
    10501 aacaactctg gaggcaacgg caataacaaa gaggaattaa cttttccaat tgcaacaaaa
    10561 aataatgatg atgcaatcga agacggcgaa ttagaagctg cggtcgcaac ggacagtcag
    10621 tttaaaggat tgttccagtg ggagttttac caagattcat atgatgcagc ttttatggct
    10681 ccttctcatg aaccgctatt tgcaaacgat gacgatttca gaatcacaga cgacggagct
    10741 gcaacgcttg atctagatga agatacgaaa aaagcgacaa tcaaagtaaa agacaacgtc
    10801 aaatggtcag atggtgaaga tgtaacggcg gacgatgtga ttttccctta cgaaatcatt
    10861 gggaataaag attacactgg tatccgttat gacgacactt tccgtaacat cgtaggtatg
    10921 gaagaatatc atgatggaag tgccgacaca atctccggaa tcaaaaaaat agacgacaaa
    10981 acagttgaaa tcgaatataa agaagtaaac ccaagcatgc ttcaatcggg tggtggtatc
    11041 tggtcatatg cagcaccaaa acatacgtta gaaggtgtag caatcaaaga tatggaatca
    11101 agcgatcaag tacgtaaaaa tccagtgact tttggccctt attacatgag taatatcgtt
    11161 gcaggagaat cagtagaata cttgccaaac gagcactact ggaatggtac gcctaaattg
    11221 aaaaagatca cgatgaaatc tgtaccgatg gcaagtgcaa ctgaagcatt gaactctaaa
    11281 ttatatgata tgattttcca aatgccaaca gatacttacg atacctataa agaaattgct
    11341 ggctatacaa acttaggccg tgaacaaact tcttatactt atctaggatt caagttaggt
    11401 aaatggaatg cagatacaaa gagtgtggaa tacaatccag attcaaaaat ggctgataaa
    11461 tcattacgtc aagcaatggg ctatgcatta gataataatg cagtaggtga acgtttctat
    11521 gcaggtttga gaagcaatgc gacttcattg attcctccag tattccagtc ataccatgat
    11581 gatgagatga aaggattcac acaagatcta gacaaagcga aaaaactatt agatgatgct
    11641 ggctacaaag atgtggatga cgatggttta agagaagaca aagatggaaa taaactaaca
    11701 atcaactttg cttcgatgtc cggtggggaa acagcgcaac cattggcaga ttactatatc
    11761 caacaatgga aagaaattgg tttggatgtc caatacacaa caggtcgtct gatcgaattc
    11821 aattctttct atgataaatt agaaaacgat gatcctgaaa ttgatgttta ccaagctgct
    11881 tggaatacag gaactgatcc aaatccaagt ggactatggg gaccaaatgc tgcgttcaac
    11941 tacactcgtt atgaatcaga agaaaataca aaattgatca acgatatcag ttcaagcaaa
    12001 gcatttgatg aagagtacca aaaagaagca tatcaaaaat ggcaacaata tgcatttgaa
    12061 gaagcatttg cgattccaac gttgttccgt aatgaagtat tgccagtcaa cgaccgtgtc
    12121 aaagactggg attggtctta tgatgtacca aacccatggg caaatgtttc tgttacagca
    12181 gattcacgtt cataatcact aaaaatgacc gaaaaagggc gatgtccttt ttcggttttt
    12241 tgatattttt ctgtttattt ctctaatatt caggtcaagg cgctgatttc atgacttctc
    12301 caataaacgg tgttcaaagt ccagtttctc ggtattaagt caaatttatg caaaaatatg
    12361 gaaagccatt ttcacaaaat tttttcttca tactcgaaac taaacgaatt tttcacaact
    12421 tctcgtcaat cactaggatt attaaagctt tacttacctt ttgaccgaac catttcaaat
    12481 ttgacaaaaa accagtataa taaggaagta tcgaaaaaat atccataaaa ggaatgtgga
    12541 aaatggacaa ccagctgaca aaagaactaa aagaaaacta taacatcgtt tttcacgatc
    12601 taaatttgct tgaacaagct tttacccatt catcctatgt gaatgagcat cgcaatctgc
    12661 aattatcgga taatgagcga cttgaatttt taggagatgc cgttttagaa ctgatggttt
    12721 ctcagtattt attccgttta tatcctcatt tgccagaagg aaaattgaca aaaacacgag
    12781 ctgcgatcgt tcgcgaagac agcttgtcga aatttgccaa agaatgtcac tttgatcaat
    12841 atgttttgtt gggcaaggga gaagagaatt caggaggacg tacgcgacca gcattgcttt
    12901 gcgacctatt cgaagctttt ttaggcgcgt tatatcttga ccagggattc gacacgaccc
    12961 atgcattcat cgaaaaagtg atttttccta aagtcaaagc aggtgctttt tcacatgaga
    13021 tggatcataa aacaaaacta caagaagtct tgcaaaaatc aggtgatgta tcaatcgaat
    13081 accgattgat caatgaagaa ggtccagctc atgaacgagt attttggatc gaagtttatg
    13141 tagatgatca attgattggc actggacaag ggaaatcgaa aaaattagca gaacaagctg
    13201 cggcggaaaa tgcattggca gcactgtaaa agtaagtgag ggaaagcttt gtatttgaaa
    13261 cgaattgaaa tagccggttt taaatcattt gcagacaaga cagtcattga ttttgaaaat
    13321 agcgtcacag cagtggttgg accaaatggc agcggaaaaa gcaatattac agaggctatc
    13381 cgctgggtat taggagaaca atctgctaaa agtctgcgag gcggaaaaat gccggatatc
    13441 atctttgccg gttccgatac ccgtaagcag ctgaatattg cagaagtcac agtgatttta
    13501 gacaatagtg atcattatct gccattggag tacaatgaga tcagtgtaac ccgccgttat
    13561 cgacggactg gggaaagcga gtttttcatc aataaacaaa gctgcagatt aaaagatatc
    13621 caagaattgt tcttagattc tggattagga aaagaatcgt tctcaatcat ttctcaagga
    13681 aaagtagaag cgattttcag tagtaaaccg gaagaccgtc gtgggatctt tgaagaagca
    13741 gctggtgtct tgaaatacaa gcagcggaaa aagaaagcag aacagaagct ttttgaaaca
    13801 gaagacaacc tcagccgcgt ccaagacatc atccacgaat tggaagaaca gttaactcct
    13861 ttggccgccc agagtgaggc agcaaaggag tttttacggc taaaagaaac actgactcaa
    13921 accgatgtct ctttgatggt tgctgaaatc aagacagcaa aaaaagattg ggacaacaaa
    13981 caagcacagc ttgcgaaatt caatctagaa ttaggcaaac tatctgagtc gatccaagaa
    14041 caagaatcca ttttggcgaa gcagcgtaaa gaaaatgcgc aagccgatcg tctgatcgaa
    14101 aaaaatcagc aagtattatt agatctttcg gaaaaactga agcagacaga aggacaaaaa
    14161 gatgttttgc aggagcggac aaagcataca caaaaaagct cgcaagaata tcaaacttca
    14221 ttagcagaag cacaaaaaaa ggttaaacat tttgaaaagc ttcaagaaag cttgatgaaa
    14281 gcagcagcag aaaaagaaac agagatccaa aaagctgaag caaatctcat aaaaacacag
    14341 caagaattag aaaaatacca aaaatcaacg aaagaattat tggcagaatt aagagatcaa
    14401 tatgttgatt tgatgcaaga acaagcagct gtaggaaacg aactgaagta tctagaacgt
    14461 caatatatac aagaaacagc aaaaagcaag cagacgttag ccaagcaatc ggaagtagaa
    14521 gcatcagtag accgattgat cttacaaaaa gaagaattga cacaaaagca ggcgcagcta
    14581 aaatcatctt taactgaaac aaaagagaag ttagaaatga tccaacaaaa cgggaaaaag
    14641 ttccaagaag cattagcaaa agaacagcct aaaatgtatc aattaatgaa tcaagtccag
    14701 cagctacgtg ctcgtaagaa aagccttcaa gaaatccaag aaaactattt tggtttttac
    14761 caaggggttc gattggtctt gcagcataaa cagcagcttt cgggtattgt cggagcagtg
    14821 gctgaactga tcgatgttcc tgcagatttc acgttagcta tcgaagcagc tttaggtggg
    14881 gcagcccagc atgtcatcgt tgaaaatgaa aaagatgctc ggcaagccat cacgtatttg
    14941 aaacagcaaa gaggaggaag agcaacattc cttccactga cgacaatcaa accaagacaa
    15001 ttaccggctc atatattgac acaagcagca gctgtagaag ggtttatagg gattgccagt
    15061 gaacaagtca gttatcccga tcaaatccaa actgtggttc aaaacctgct aggaacgatc
    15121 cttttagcca aagacttaac tagtgccaat gcaatcgcgc aaacgatccg ctatcaatat
    15181 cgagtggttt ccttagaagg tgatgtgatg aatgccggag gatccatgac aggtggagcg
    15241 aataaacgag gaaaccaagg cagtttgttt gtacaaaatc aagaattgaa acagttgact
    15301 agtgaatttg aagaagcaga taaacaactc caagcacaag aaaaaaaagt tcaggagctg
    15361 caacaagaaa cagctcgttt agccgaagaa caagaagttt tgcgtacacg aggcgaacaa
    15421 ttacgttttg aagaacaaga agcgacaaat cagctacaaa acatcataaa tgaacttgaa
    15481 cgttttgaaa aagaaaagca aatctcaact tttgagacac gtgaactgca gcagtttatc
    15541 gaagattacg aaaagcaaac gaacgaattg aaagacaaac aaacagatct tgaatcgcaa
    15601 cgacaaaaaa tcgacgaaga aatcaaatcg ttaagtcaag aaagcgacca gatggaagcc
    15661 agaagagcac aagtacaaag tcaaaaagca caagaacaag cagatcttgc tgtcttgaaa
    15721 gagcagttta atcatctgca gatccagctc cgcggtgccc gagtccaaaa agcagaggca
    15781 accgaacgtc aagaagcgat agaaaagcag ttggcgacat tgacagcaga cttttctgat
    15841 catgaagtca cagaagaaag tttggaaaaa caaattaacg aattatctgc tcagcgagaa
    15901 acattaaaag ccgagttggc aaaagccaaa gagcagcgag atagaaggca aaaggagatc
    15961 gataagcttg aagcagtctt ggcagagcgt aatcgtgagc aaaaagcacg actgtcggaa
    16021 caatccaaat tagaagtcca aaaagaccga gctgaaatga ttttagataa tcatttgacc
    16081 tatctgcagt cagaatatca gctgacattt gagaaagctt cccaagacta tcaagaaaca
    16141 accgatatcg aagacagtag aacaaaggtt tcatccttaa aagaacagat tgaaaaactg
    16201 ggaccagtaa acttaaattc aatcgaacaa tacgaacaag tcagtgaaag acatacattc
    16261 ttagcaacac agcgagatga tctgttagca gcgaaaaacc aattgtttga aacgatggat
    16321 gagatggatg atgaagtgcg gacaagattc aaagaagtat ttgaagctat ccgtcaagaa
    16381 ttcaaaattg tctttccaaa tatgtttggc ggcggtaaag ctgaattagt actgacggaa
    16441 ccaactgatt tgctgaaaac aggtattgag attgaagtac aacctcctgg caaaaaactg
    16501 caaagtctaa gtttgctttc tggaggagaa cgagcactta cagccatcgc gttgctcttt
    16561 tccattatcc gtgtctgtcc tgttccgttt tgtatcttag atgaggtaga agcagcgtta
    16621 gatgaagcca acgtgaaacg ttttggtcgt tacttgagcg atttccaaga tgacacacaa
    16681 tttatcgtag tcacacaccg caaaggaacg atggtcgcag cagatgtact gtacggagtg
    16741 acgatgcagg aatccggtgt atcaaaaatc gtttctgtac ggatggaaga tatcaatgaa
    16801 gaggcgaaaa tcaacgaaac gaaagtgtag gaacgaaaat gatcaaatta gtagcaatcg
    16861 atttggacgg tacattatta aatgaacaaa agctgatttc agatgagaat aaacaagcat
    16921 tagcacaagc aaaacagcaa ggggtcaaga tcgttctatg tacaggacgc cccttagctg
    16981 cgatggcgca ttatctgcaa gaattaggac tggtagatga aggtgactac agcatcactt
    17041 tcaacggcgg tttggtacaa aaaaatgata ctggtgaaat catggaaaag aaagtgatgg
    17101 aagtatcaga tatccaacgc ttatacaaac tggctcagca attggatctg ccactagatg
    17161 ttttgtctga caatgtggtt ttacagctgc ctagtgcccc aaggaagcaa tccttgtaca
    17221 atgtgttgaa taagctgctg caattccaac cagcggatat aagcgatatt acagatgaat
    17281 ttgtgcttaa taaggcagta atcgcttatg accaagaaga attagatcct aaaatcaaag
    17341 aaataccagc agaattccat gaaatgtatg agatcatcaa aacaagaagt atgcttttgg
    17401 aatttatgcc aaaaggtgtt acaaaagctt atggtatttc tttattggca aaagatcttg
    17461 gtttgaaaca agaagaaatc atggcgatcg gtgatgagga aaatgattta ccgatgatcc
    17521 aatatgctgg actaggcgta gcgatggaga atgctgtgcc tttcgtcaaa gaagcagcag
    17581 atttcgtgac agccagcaat gaagaaaatg gcgtagccaa agtgatccaa aaatttgtat
    17641 tagagtaggg ggagtcaaat gggatttttt gataaaatca agcgtgcttt tactggcgaa
    17701 gagaaaacag aagaagttga agtacaggag aagtacgaca agggtttaga aaagacgcgt
    17761 aaaacatttg gtgaacggat gaatgagttg tttgccaatt ttcgaacagt cgatgaagac
    17821 ttttttgaag aattagaaga aacacttatc ggtgcagatg taggttttga aacagcgata
    17881 cgaatcaccg aagcattacg tcaagaagtg aaactcagaa atgcgaaaaa atcggcagaa
    17941 gtccaaaatg cgatcatcga gaaacttgtg gatctctatg aagcggaagg aatcaatgaa
    18001 gtaaatgaac taaatatcca agaagacgga ctaacagtta ttttgtttgt cggtgttaat
    18061 ggtgtaggga aaacaacaag tattggtaaa cttgcccatc agttcaaaaa cgaaggtaaa
    18121 aaagtattga tggcagcagc agataccttc cgcgctggag caatcgacca gttagttgtc
    18181 tggggcgaac gcgctggtgt tgaagttgtt cgtggcaatg caggaggcga tccagcagct
    18241 gtcgtattcg atgctttgga aagagcaaag acagagcagg cagatgtttt actagttgat
    18301 actgcaggcc gactgcaaaa taaagtgaac ttgatgaacg aattagaaaa aataaaacga
    18361 gtaatccagc gggaattacc agaagcaccc caagaaacac ttcttgtatt agatgcaacc
    18421 actggtcaaa acgcgatggt tcaagcaaaa caattcaaag aaacaacaga tgtgaccggt
    18481 cttgttttga caaaattaga tggtacagca aagggcggta tcgtacttgc tatccgtaat
    18541 gaattgcatt taccagtgaa attggtcggt cttggtgaag gaatcgatga cttggagccg
    18601 tttgatccaa atgattttgt cgttggattg ttcaaaggat tactaaaaga agaataaccc
    18661 atgtctaaaa agcaggagct gtgacaaaag ttgcagctcc ttgtttgaat gattgttttt
    18721 aacatagctg tgtttttgat gccttcattt ttatacttat ttgtaagcgg ttctcaaaca
    18781 atttcgttct ttttcttaga ctttttgatt ataattagat tcatatgaat tggaggtcac
    18841 ttaatgatga attttaagca attattattg catgtcaatg cgcgcccttt tatcgaccaa
    18901 gctcgatttg gtatagaacg ggaaggacag cgggttgatc ttgcaggaaa tctagcaaaa
    18961 accgatcatc cagcaatctt tggcgatcga tcctatcacc cctatatcca aacagatttt
    19021 agcgaaacac aaacagagat gatcacccct gttaccgatt ctattcccga attatttcag
    19081 tatctcgctg ctgtttatga tgtgactgct cgttctatac cgaaagaaga gatgatctgg
    19141 ccattaagta tgccacctgc cttaccggaa aaagacgaag agatcattat tgcaaaatta
    19201 aaaaatttcg aagatgtctt gtatcgacgt tatttagcaa aagaatacgg gaagcgaaaa
    19261 cagatggtaa gcggtattca tttcaatttt gaatttggcg atgagttgct aagaacattg
    19321 ttcagccatc aggaagaatt tcaagatttt tccgaattca aaacagaact ttatttgaaa
    19381 acagcgagaa actttatgcg ctatcgctgg atgatcactt atttattcgg tgcttcacca
    19441 atgagtgaga agaactactt tttagatgaa tcacatccgc aagaacctgt tcgcagtatt
    19501 cgcaacagtg cgctgggcta tacgaatcat ccaaatgtga aagtttccta tgcttctatg
    19561 aaacagtatt tagcagatat cgagcgaatg atcgaagaag ggaaactttc ggaggagaaa
    19621 gaattttata caccgcttcg tttccgaggc ggaaagaaag tcgcagattt agcaacaaca
    19681 ggtgttcgtt atattgaatt gcggaatatc gatttgaatc cttatgcaag attgggtatc
    19741 aatccagagc aagttcggtt cttacaatta ttcctgatgt acatgttatg gacagaagaa
    19801 aaagaagact gtgaccaatg ggtagcagaa ggtacgactc gaaataacaa agtagcacta
    19861 gaacaaccat cagatcaaac agaatttcat caagaaggta gagaaattct tgaaggaatg
    19921 aagcaaatgc tggttgaatt ggattggttg gattctcttt atttagtaga ggaagcattg
    19981 actcaaatgg atcatcctga acaaacgtta gcagcgaaac tctatcaaga agcacagcta
    20041 tcgagtcagc aagaagttgc tgtggcattg ggacatcaat attataaaga aagccatgaa
    20101 cgtccctatc agttggccgg ttttcgcgaa atggaacttt ctactcagat atttatgttc
    20161 gatgccatcc aaaaaggtgt ccaagtcaaa gtattagatg agtcggatca atttttgcgt
    20221 ctgcaattcc aagaccatgt agaatatgtg aaaaatgcaa acatgacaag taaggacagc
    20281 tatatcgtcc cacttatcat ggaaaacaaa acagtaacga aaaaagtctt aaaagaagct
    20341 gggttccggg taccaggagg cgcagaattt tcatccatgg aagaagcggt aaaagcttat
    20401 ccgagatttg ccgagcaggc atttgtgatc aaacctaaat caaccaatta cgggttaggt
    20461 atcacgattt tcaaggaagg agctagtctg gaagattatc aagcaggttt agccattgct
    20521 tttcgtgaag acagttcggt tttagtagaa gaatttatgc cgggaacgga atatcgtttc
    20581 tttgtgatcg acggagaagt ccaagccatc atgttgcgag tccctgctaa tgttattggt
    20641 gacagtatcc gaacagtgaa agaacttgtt gaagaaaaaa atagtgaccc tttgcgtgga
    20701 acgaatcatc gtgcaccttt agaattgatc caattaggtg aattagaaca attgatgttg
    20761 aaagaacaag ggttgacgat cgaatcagtt ccccaagcca atcaaattgt ttatctaagg
    20821 gagaactcaa atatcagtac aggtggtgat tcgatcgata tgactgatga attttctgaa
    20881 gcttacaaaa aaatcgccgt ttctgcggta gaagcactag gagctaagat tagtggtatc
    20941 gatttgatta taccagataa agaaatcgat cctacaactg ataaaaaagc atatgggatc
    21001 atcgaagcaa atttcaatcc agccatgcat atgcatgttt atccttttgc aggaaaaggc
    21061 agaagattga cgatgaatgt attgaaattg ttatatcctg aagtatttta aaagaaaaag
    21121 cttttgacag attgtcctat ttgatgtcaa tctgatcaaa agcttttttt gcatgtaaaa
    21181 atttatgtgt tttttcgttt taaaataaac gatcccagaa gctttttttc ttttttggtt
    21241 tttctttttc agctggcgaa gagttttctt ctgggtattt tttttcgata tcaatcacag
    21301 gttcatttac tgcttctttt gctgttaaga gcaatccgaa ttcctcagga tttgcagtga
    21361 cgtaatcgtt gactacagtg aaacggctat tgtttttcgt tgctagctgg atatattggt
    21421 tttggacaga aaggggtaga gccccattca agagtatgga agcatctgga tgttttttga
    21481 gttcttttaa aaagttttct ttgttttttg gttggatcat ttcagcaaca gtcatactaa
    21541 gatagcagcg ttccctgaat gtccctaaat atttatgctg ttcttcaggg ttcactaaag
    21601 gtgtaccata aagtccctta tccaaatggc tttttacatc atcttgtgcc ataaaaaata
    21661 ccctccttct tctctttgtc tttcttacga ttatagcata ttcccaataa tacctaaaac
    21721 atatgaaaaa gcggttgctt ttgttcaacg attcacttaa aataagaatg aaaatcattc
    21781 tcaattagaa aggtggattg gatgcgctac ggacgactag tagcattatt agttatttta
    21841 agtagtattt cattgttcat cggagtatcg aatgtacaat tgtcagaact gctccagtta
    21901 gatgggcaaa gcttacaaat cttgctagtg actcgtttgc cacgaacgat ttgtttgatc
    21961 cttgtgggag cgaccagtag tatttgtggg ttgatcatgc aacatttgac ccaaaataaa
    22021 tttgtttcac caacgactgc gggcacgatg gatagtgcgc gtttaggtat tttagtggct
    22081 atgattttcc tacctggtgc atccttgatt gtccgatcct tgacagcatt ttgctttgct
    22141 tttgcaggaa cattgctttt tttatccctt acgcgattgt tcctccagaa aaatcaattg
    22201 atgcttccgt tgattggagt catgttaggg aatatcattg gttcagttgc tacttttttt
    22261 gcttatcaat tccagttggt acaaaatatg acttcatggc ttcaagggaa ttttgcgact
    22321 gtgatacgag gaaattatga gttattgtat gctgttgttc cattgttgct cttggtttgg
    22381 ctatttgctt accgatttac gatcattggt ctgggcgaat cgtttgcagt aaatctggga
    22441 atagattacc agaagatgca gtttttaggc attggcttag tatcattggc tagtgcagtc
    22501 atgttgatca tggtaggaag cattcctttt atcggagtga tcattccgaa tcttgtctct
    22561 agaatatatg gagatcaagt gcacaaaaca attggcgtaa ctgctttagc aggtagtttg
    22621 ttcttagtat tttgcgatct tgtcgctcgc gtgttgattt ttccttatga aataccagtc
    22681 agtgtgatcg ttggaatcat cggtggggct atcttccttt atcttctgat aaggggacga
    22741 cgatatgctt aaaaaaaagc catctatttt tattggtatg cttgttcttg ttttatttgt
    22801 tacaggaatg tacttgacga tccatacata tggaaactgg cattttgcat gtgcattgag
    22861 gagtaagaaa atttttgcat ttatccttgt ctcgctagct gtcagcagtg ccacaatcag
    22921 ctttcagaca attacgcata accagttttt gactccttca gtattgggac ttgagtcgtt
    22981 atatgtcctt gtccaaacgg tccttttctt ctttatcgga ggagtgacga tgcttcagca
    23041 gacaggtaca gtttttttct tattgaacgt tgggctgatg gtcgggctga gtctattgtt
    23101 atttttcttt cttttgaaaa aaatgagagc gaatcttttt ttattactga tgtcagggat
    23161 gattttaggt acgctttttg gtagtgtcag taccttttta caagtcatca tggatccgaa
    23221 tgaatatgat ttgcttcaag gcagattatt tgcaagcttt ggcaatatcc agagtcagta
    23281 tcttttgcct gcttctttga tcattatcgg ctgctggtac ctgttatttc gatatcgaca
    23341 tgtgctagac gttttccatc tcggaaatga acaagcagta aacctcggga tagacacaga
    23401 tcgttttgaa tttttgattt tatgttgtgt cagtcttttg acagggacat caacggctct
    23461 tgtgggtcca gttacttttt tagggtttat tgtcgcaaat atcagttatc gtgtcttttc
    23521 tacttatcgt catggtcagc tatttatcgg aagctttctg gtagctatgc tgtttctgac
    23581 aggaggacag ctcgcagtag agcagatttt cggctggaat acaacagtga gtgtcgtcgt
    23641 tgaatttgtc ggaggaattt attttgtcac aaaattgatt cgcgaaagga agagtatcct
    23701 atgattgatt tacaaaaggt caataaactg taccatggaa aatcagtggt taaagatgta
    23761 cagttaacga ttcctgaaaa gaagttgact gcttttatcg gtccaaatgg tgctggaaag
    23821 tcaacggtgt tgtcgatgat cagtcgtttg ataccaaaag atacaggtga aatctattta
    23881 gaccataacg aagtaaaggc ttggaaatcc aatgaattag ccaaaaaatt atcgatccta
    23941 cgacaatcga atgaaataaa tgtccaaatc accgtgaaag aattagtaga atttggacga
    24001 tttccttata cgaaaggaaa attgacgaca aaagacgagg aaatcgttca ggaatcactg
    24061 gaacacattg ggttagaaga aatagcggat caatcgattg acactctttc aggcggtcaa
    24121 ctccaacgtg cgtatattgc aatggtgcta gcgcaagaca cggattatat tttactggat
    24181 gaacctttga acaatctaga tatgaatttt gcggttcaaa tcatgcagat tttaaaaaat
    24241 ttagtacaag aaatcgggaa aacaattgtt attgttcttc atgatatcaa ctttgctgca
    24301 agttatgcag acgaaattgt tgcaatgaaa gatggacagt tatttacgca aggaacgacc
    24361 caagaaatga taaaaaaaga tgtattggat gaattatacg aaatggacat acggatttgt
    24421 gagattgaag gccgcagatt ctgtctttat ttctaaaact aacattagga gagaagagat
    24481 gaaaaaatta gtagtaggta tcttgattct agcatcattt ggtttagcag catgtggaaa
    24541 tagcaacaac accagtcagg cagacacaaa aaaaagcagc acacaaactg aaactacatt
    24601 gacgataaca gatagtaatg gagaccaaat cgaggtgcca aataatccag aaaaagtcgt
    24661 cgtattcgac aatggttctt tagatacgat ggatgctctt ggtgttggag ataaggtagt
    24721 tggagcagct acaagcagcc ttcctgagta tctttcatcc tataaaaaag tagaatcagc
    24781 aggtggaata aaagaaccag acttagaaaa aattaatcag cttcagccag atctaatcat
    24841 catttctggc cgacaaagag attttcaaaa agatctaagt caaattgctc cgaccatttt
    24901 cctttcttta gatgcgaaaa atccatggga atctttccag caaaatgtca ctgctttagg
    24961 ggagatcttt ggtaagcaag aagaagctaa aactcaatta gaggaattat ctagtgcaat
    25021 cgatcagacg aaaaagaaag cagaagctac tgacaaaaaa gcactagtca cacttgtgaa
    25081 tgaagggcaa ttatcagcct acggatctgg ttctagattc ggtttcatcc atgatttatt
    25141 tggctttgaa caagcagatg atcaaatcga agcatccact cacggtcaaa gtgtttctta
    25201 tgaatatgtg ctggaaaaaa atccggatat cttatttgta gtagacagaa caaaagctat
    25261 tggtggagac gacagcaaag atgatatttc tgccaatgaa ctggtagcac aaacgaatgc
    25321 tggtaaaaat cagcagatca tctcccttga accagatgtc tggtatttaa gcgggggcgg
    25381 tttagaatca atgaaactga tgatcgaaga tgtgaatcaa gcatttaaat agcataaata
    25441 acagaggatt aaactctcat actatgtaac aataatgttt cttacattct taaatagcaa
    25501 gagcctatcc tcaaagaagt tatcaaaaac catccgtaaa atcgtattga tgattttacg
    25561 gatggttttt atttatttga gaaatctttt ttcaacgtga attcattatc tacatcatac
    25621 gggttgattc tagaatgggg agtggcaaaa aatagtgtga tccatttttt cagcatcaag
    25681 atcgcctcct ttgatttgaa tcaaaggtac aaagcgaaac ttagataaaa cttaaattta
    25741 gatattttta ttttgtaccg atctcttctt taaacgatgt tccatctgct gcttctcggc
    25801 aataagtcca ctagactaga aaatatgaga agctattttc tgtccagcgt tcttattact
    25861 caaaacaaat cgcagttgtc acaatctctt ctaaacgatg ttccatctgc tgcttctcgg
    25921 caataagccc actagactag aaaatatgag aagctatttt ctgtccagcg ttcttattac
    25981 tcaaagcaaa tcgcagttgt cacaatctct tctaaacaat gttcaatcag ctgaccttcg
    26041 gtattagctc aaagcactga tttcacaacc tctatttagg catgtgtctc agcaagcggt
    26101 tgatagttga tgatctgcat ttttcgataa aaatcggcgg gaagcttact ccaccgtttt
    26161 agtaattgat agaaataact ctcttctgct ttgattttat actcgcttaa tatcaatcca
    26221 aaactattcg ttttttttga agctaatttt ttataaatat ccaatgcttt ggttttatct
    26281 tcattgacaa agagtgtttt tacttcaata taagaaccct cgatattttg tgtttcttcg
    26341 ataaaataga tgttttttat atattccttc atagtgattc ctccaaagaa gtcttttatt
    26401 tgataagttt actctaccga agagttgtta ataatccgtg aacaaaaagt aattcttttt
    26461 ccaatagttg ttaaaggttt tttatttttt atagtatttt ttgttattta agaaaggaat
    26521 aaggttaaac aaataacagg ttgctttttg ggtctttttc cgttcaagaa actgaacgaa
    26581 gaaaagggaa agttgtctct tttgtgataa gagaaatcat aataaacgaa tgacttactt
    26641 tccaataaat tgagaacagt cagaaccaga caaattagtt gcatctgatg aataaagtga
    26701 ttattctatg acatgaggtg atacagatga aattagaagt gacaaaagaa gcacaagaag
    26761 tattgaagaa taaattagaa gcagacgatc aacttttatt agatatcgaa aacggtgacg
    26821 gtccatttgc agaaagccaa gtcagttgcc aattggatac ggcatttcga ttgatcattg
    26881 tcaaaaaaga tacacaaaca gatctaagtc tttattctgt agtggctgat acaccagttg
    26941 gaccaatcaa aatcaaagac agtgcaatac tttacatgga tgatccatcc acacttgctt
    27001 tagaaccaac ttataatagt ttacaattaa aaggaccaag cggcttgaga aaaggcaact
    27061 tgcaagttgt tcgaaaagac tagagggata aaatgggaaa attggcgatc atcagcgacc
    27121 tacatgtgga catcaataaa ttcggcgaat cagagctgat gcagctttgg caagttttgc
    27181 aaaagcacaa gatcactcgg cttcatctag ccggagatac ggctaataaa gtagaccgtt
    27241 gcctagctgt tgtcgaattt ttcgtcaata aaggattatc tacgacattt catttcggca
    27301 atcacgagtt ggcagatgtg cccaatgaac agatgatgga ccattttcca gacccacatt
    27361 ttttaaatga acggtatatt gctttaaatg agcagaccgt tcttttaggg atgaatggct
    27421 ggtacgacta tcagttttcc gaattgaaag attacgaggc aatcgtacga atgaagcgtt
    27481 tgtattggta tgatcggttc atccagcgtg ggaaaacaga tctcgaggtc aatgcagaaa
    27541 tgcttgcagc tgcaaaaaat ttgctagacc agctggaagc aaaaaaactt gaggtgattt
    27601 tatccactca tttcgttcct aaacgggcat ttatcatgta tcaatctgct ccttatgagc
    27661 gttggaacaa attaaatgca tttttaggat cagaatcatt cggagaatta cttgaccgtt
    27721 attcaaatat caaacaagta gtttttggcc atacgcatcg tcgctttgaa gataaatcca
    27781 tcggtcacac gatttacagc tgcagacctt ttggttatta ttatgaatgg cagctgaccc
    27841 gagattttgt cttgaaagaa cagttgctag acagctatga tcctatgaaa ttacgtgtcc
    27901 tcttgcgtgc ccatcaccca gcttttcagg cctatcaaac acaacatttg caagaggaat
    27961 ttgaacaagc gatgacaatc gttccgtatt gagacagcat tagtaatgtg taaaagaaaa
    28021 taccttgtaa aacataagga taagtaagag cagtagatcc atttatcgtg gatatgctgc
    28081 tttttttgcg gaaaataatc agcgaatgtt tatttgatcg gatgatttgt aactcgcaga
    28141 gtactagatg caagcgtgta ttcttttttt gaacgataat cacaaaaaaa aatataatat
    28201 acatttttta tttaattata attaatattg attgaagtct aggcagtcaa gtcgagagtg
    28261 taaaatattt tgtgtaaatg aaaaaatcca tacaaaaaag gaagtc
//
LOCUS       DAZKLM010000029        27632 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3764
            SAMEA115706913-rid22582563.denovo.029, whole genome shotgun
            sequence.
ACCESSION   DAZKLM010000029 DAZKLM010000000
VERSION     DAZKLM010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706913
            Sequence Read Archive: ERR13196647
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27632)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27632)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287823.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287823.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,004
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,849
            CDSs (with protein)               :: 2,849
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 41 of 96
            Pseudo Genes (incomplete)         :: 52 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 15 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27632
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706913-rid22582563.denovo.029"
                     /isolate="VRE3764"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(79..606)
                     /locus_tag="ACG842_001153"
     CDS             complement(79..606)
                     /locus_tag="ACG842_001153"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HFQ0247712.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQKLAEKVVDL
                     PVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYDGLPSKDENFQKAR
                     LAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIRVINAYFHPMPNEG
                     FFTFMVDNTELVSYE"
     gene            complement(673..2175)
                     /gene="eat(A)"
                     /locus_tag="ACG842_001154"
     CDS             complement(673..2175)
                     /gene="eat(A)"
                     /locus_tag="ACG842_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection-like protein
                     Eat(A)"
                     /protein_id="HFQ0247713.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            complement(2433..3554)
                     /locus_tag="ACG842_001155"
     CDS             complement(2433..3554)
                     /locus_tag="ACG842_001155"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HFQ0247714.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            3680..4621
                     /locus_tag="ACG842_001156"
     CDS             3680..4621
                     /locus_tag="ACG842_001156"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HFQ0247715.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            complement(4771..5667)
                     /locus_tag="ACG842_001157"
     CDS             complement(4771..5667)
                     /locus_tag="ACG842_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HFQ0247716.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMNFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPILSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
     gene            5936..7276
                     /locus_tag="ACG842_001158"
     CDS             5936..7276
                     /locus_tag="ACG842_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFQ0247717.1"
                     /translation="MEDKKLKKQLSSRHITMLALGGAIGAGLFKGSGEAIGIAGPSVL
                     IAFLIGGLILYIVMKGLGKIVLSGGDTHHGLSGLIRPYLGAHSADFTDWVYWSMWIIN
                     IIAEAVAAASFLQLWFPHIPAWVFVFMLAVLTTIINLYSVRLFAETEYWLAFAKISVI
                     ILLIIFATYLVGQQMLGTGVFPTLQQLTDHGGFTPHGMKGIISSLLVVIYSYGGSELI
                     AITVSEADDPKRAIPKAIKGVMGRIISFYIIPLFLLLILFPWNTLAGTNVSPFVMVFE
                     KMNIPFAADIVNFVIVLALFSSINSGVYASSRILFFRLKDRKGSSRKLAVLNKHQVPQ
                     RAVLFCTSVLYLGVALSYFVGDKLFGYLAGSLSYTVLLIWIIISAASFVLALRKGTLW
                     DKGISFFALAVLGLIFLGILFTNSIGVTVLTGLLYLFIYFSYQKKNDAFVLTNE"
     gene            7494..7937
                     /locus_tag="ACG842_001159"
     CDS             7494..7937
                     /locus_tag="ACG842_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HFQ0247718.1"
                     /translation="MKKYWKFLLMIGVSTVVMFGMMYLNVYALDHIFFSRTRVFMALM
                     MGAVMAVIMLLFMWKMYENKKINVMILAASLLVFAGSLFMVRSQTTVNDTAWMKAMIP
                     HHSIAILASKRADLKDPEVKKLAEEIIQAQEEEIDKMKKMIKEME"
     gene            complement(8441..9859)
                     /gene="nhaC"
                     /locus_tag="ACG842_001160"
     CDS             complement(8441..9859)
                     /gene="nhaC"
                     /locus_tag="ACG842_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HFQ0247719.1"
                     /translation="MGKKGTPSFLEATLVLMVIVLGIAIGVVGLKVSPNITILAAIGM
                     VMLYAVIKRYPTDWLHEGIINGIKPGIIPIFIFILVGSLIAVWIQAGIIPTLMVIGFK
                     LISVQWFVPSVFVVCAIVGSAVGSAFTVMSTIGIAFFGIGTTLGLNPALVVGAIVSGA
                     VFGDKMSPLSESTNLAAAIVDADLFKHIKHMMWSTVPAFVVSFFLFMILGHTSKAASL
                     SSIREVTDVLEANFTISIWSLIPLFLMLLCAWKKVPAILTILLNIAVAVGMIFIQNPQ
                     TSLTKLAGVIESGFVSNTGNQQIDSLLSRGGIESMMPTVSLIILTLSLGGLLIEFGLI
                     STVMERVSRKMTNTPNLIFTTLMTSIGVNIFIGEQFLSVILPGNAFKETYEKAGFDPT
                     VLGRTLEDGGTVINYLVPWGIAGSFVASTFGIPTLTYLPFVFFSLLSPVFSMVSAFTG
                     WGISKKFVKPQEAVSKTAQLEQ"
     gene            complement(10262..11485)
                     /locus_tag="ACG842_001161"
     CDS             complement(10262..11485)
                     /locus_tag="ACG842_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HFQ0247720.1"
                     /translation="MTTTISTNQLKDLIQYRLPEYQALALDIHEHPEVSNYEFYSSAA
                     LIKQLEKERFWVEKEVAGHRTAFDARYISNKPGPTIAFLAEFDALPGIGHACGHNLFG
                     TYSVLAASIVKQFIDETGGEIRVYGTPGEEGGENGSAKGSFVREGFFEDVDVALCVHP
                     AFRYGKTTESLANDPVDIKFYGVASHAAAAPEKGVNALEALIQVFNGINALRLQLPKD
                     VNIHGIITDGGVAANVIPDYAAGRFYLRAGNRQTLDKVYEKVENIVRGAAISTGTTFE
                     FGLFQNAVDDVIVTPSFDDLFFQHAKEAGVPDEEIETEQRTSLGSSDVGNVSQVIPTI
                     QPTVAISETYIAGHTEEFKAAAKSDKGLASIAIAAELLANTALDLLKEPKLLERIKQE
                     HQEIVARKKQEGATF"
     gene            complement(11516..12355)
                     /locus_tag="ACG842_001162"
     CDS             complement(11516..12355)
                     /locus_tag="ACG842_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HFQ0247721.1"
                     /translation="MKKIIRFVVITFVAIIWLAGCSQGKADTTDKKETIVKLGIIGED
                     TDVWDNVKDRLKKENINLEYVKFTDYNQPNVALADGSIDLNSFQHQFFLDNYNKEHGT
                     DLVSIGNTVNAPLGIYSNTLKDVADIKDGAKVAIPNDVTNGGRALLLLQTAGLIKVDK
                     AKGQAPTVSDITENRKNLDISELDASQTARALSDVDISVINSGVAVDAGFNPASDAIF
                     LEPVDDHARPYVNIIVARKEDENNKTYQKIVDAYQTEDTVKVIEKTSKGSSVPAWETF
                     GRK"
     gene            complement(12372..13052)
                     /locus_tag="ACG842_001163"
     CDS             complement(12372..13052)
                     /locus_tag="ACG842_001163"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease"
                     /protein_id="HFQ0247722.1"
                     /translation="MTDFFKTYLPNVYLIPDEFVEATKQTLYMSFWTAFIGGMIGIIL
                     GVTLVVTRPNGLLANRLLFEILDKLINIIRSIPFIILLSLLALTTRFLVGTTIGAKAA
                     LVPLIFGVVPFFARQIENALLEVDPGVIEAAEAMGTSPIGIILRVYLLEGLPGIIRVS
                     ALTIINVIGLTTMAGAVGAGGLGNLAISRGYNRFQTDVTIVATLLILILVFLSQFISN
                     QLIKKITH"
     gene            complement(13054..14127)
                     /locus_tag="ACG842_001164"
     CDS             complement(13054..14127)
                     /locus_tag="ACG842_001164"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HFQ0247723.1"
                     /translation="MIELRDVSVCFKQKEKEIQAVDSVDLTIEKGDVYGIVGYSGAGK
                     STLVRVMNLLQKPTAGEVIVNQTNLSQLSPKELRKERKAIGMIFQHFNLMESRTIFDN
                     VDFSLKYSGKSKQERRQKVNELLELVGLEEKASAYPKQLSGGQKQRVAIARALANEPK
                     VLLCDEATSALDPKTTLQILALLKKLNRQLGLTIVLITHEMQVVKEICNKVAVMEDGR
                     IVEKGSSIQIFSNPEEELTKDFIRTATHLDQALETIIAHSAFADQIANKWLVELSYIG
                     NQTNEPLIAHLYSKYQVTANILYGNVELLQETPLGSLIVTLAGETKQRKKALDYLIKS
                     GVKINILQKNDLDSRIKVIEGGR"
     misc_binding    complement(14183..14421)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     misc_binding    complement(14464..14697)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(14774..15445)
                     /locus_tag="ACG842_001165"
     CDS             complement(14774..15445)
                     /locus_tag="ACG842_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFQ0247724.1"
                     /translation="MKGAIFDLDGLLVDTEKTYRDGWLWGFQQFDIHIPKEIVDAWGG
                     KNWKQSFDILVKAAGSPEKVQEVRAKREEYFYQQLTSGAIHLKPYAKEILADLKERHM
                     KLGIATTTVTKRATDILDHFKLADYLDTFTFGDEVSENKPSPIPYLTALERTGLEASS
                     AFAVEDSLVGATAASRAGLGVVLIPDSSFVRNYTVEEKEKLKLLAEGKDLRTVIEMLN
                     KKTEK"
     gene            complement(15532..18180)
                     /gene="mgtA"
                     /locus_tag="ACG842_001166"
     CDS             complement(15532..18180)
                     /gene="mgtA"
                     /locus_tag="ACG842_001166"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015444 - P-type magnesium transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HFQ0247725.1"
                     /translation="MEKKKDTQKIYAAFAGKTKQELFELFHASANGLTEEQIERSREN
                     YGENAISYGKKTPFIVEILKAYITPFTLVLIALGIISFITEYVLAAPGDKDLFGVAII
                     FTMVIVSGTMTLIQSVRSNQAAEKLKSLVKVTAAVKRNGKYTEIPMEEIVCGDLVRLS
                     AGDMIPADMRLLSAKDLFISQAALTGESYPVEKHAKVCEDDQQSETSYENLVFMGSNV
                     ISGSAEGIIVSVGNETLFGHVAETLSEKPIQSSFELGIHKTSMLLIKFMSLMAPAVIV
                     INGLTKGDWLEAFLFGLSVAVGLTPEMLPMIVTTNLVKGASVMAKKGTVIKNLNAIQN
                     FGAIDVLCTDKTGTLTQDKIILEYHMDCEGNEDDRVLRHAYLNSYYQTGLKNLLDVAI
                     IDEATETLDTDKINYQKVDEIPFDFERRRMSVVVADPAGKTQMITKGAIEEMLNISKF
                     IDLGGTVTPLTKERKEAVLAKVQSLNEDGLRVIGVAQKTNPSVVGEFSVKDESDMVLI
                     GYLAFLDPPKETTKQALKALKEHGVAVKVLTGDNELVTRSVCRQVGLEINELVTGEKI
                     SEMSDHELAQVAEDHEVFVKLNPQQKARLTTALRKNGHTVGFLGDGINDAPAMKVADI
                     GISVDTAVDIAKESADVILLEKDLTILERGLLSGRETFGNIMKYIKATASSNFGNMFS
                     VLIASTFLPFLPMLPLQLLFLNLIYDVSCISLPWDRMDKEYLDEPKKWNASSIGEFMV
                     YFGPTSSIFDITTYLLMYFVICPAVVGGSFHTLDSSQRVIFIALFHAGWFVESLWTQT
                     LVLHALRTPKVPFIQSNATFAMFVITTLGIIVGSVLPFTSFGAELGLMPLPGNYWLWL
                     FATVIAYLALVTAVKKIYIRRFQELL"
     regulatory      complement(18522..18696)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00380"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="The M-box (ykoK) riboswitch appears to function as
                     a genetic 'off' switch that is important for maintaining
                     magnesium homeostasis in bacteria; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="magnesium"
                     /db_xref="RFAM:RF00380"
     gene            18869..19306
                     /gene="ndk"
                     /locus_tag="ACG842_001167"
     CDS             18869..19306
                     /gene="ndk"
                     /locus_tag="ACG842_001167"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HFQ0247726.1"
                     /translation="MERTLVIIKPDGVRRHLVGSIIQRFEAKGLAIAEMKFETMTPEL
                     AKEHYQHLTERSFFDELIDYMTSGPVVYLVLVGEEVIDIVRKMVGATKAADAVPGTIR
                     GDYALPGTENIIHASDSRDAAVKEIARFFPPFDINKPSEKAAK"
     gene            complement(19328..20194)
                     /locus_tag="ACG842_001168"
     CDS             complement(19328..20194)
                     /locus_tag="ACG842_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFQ0247727.1"
                     /translation="MYDGELIKKLRVNKKLTQSQLAEGICFKTSLVGIETNSVKKMSF
                     VTLRSFLERMNMTLAEYEWMRNQLDEPIKLKKKRRMLDRVQEDSFDPYKEISNNRRHF
                     KKTEDLYYLVLNLHMVEKTEVSETEFEFLRYECRIIEEYLMQIPDYGQFELSILADFP
                     WIFSDRFIENNYLKIKKRMRRLAQLNNHEQYLFTFLMNLTSFYIENGRLKKARSVNED
                     MKRSLAHKERSTVIYETLMADYYQRLISAALGEEKTKDYQEFFTVLEYMLGKNQRRSL
                     EKKLLAIAEPSN"
     gene            complement(20354..22447)
                     /locus_tag="ACG842_001169"
     CDS             complement(20354..22447)
                     /locus_tag="ACG842_001169"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HFQ0247728.1"
                     /translation="MTQLKKLCQNRLAIVLTAVFFFWLKTIIAYYADFSLGVEGTIQY
                     FILWINPIATTLLFFGLSLYIKKPKPALAILLIIDILNTLLLYLNIIFYREFTDFITV
                     KSVLGFSKVSQGLSGSSFSLMKPHDVIYWLDIAVFIGLLVWLKVKKIPIKSNPVGKPM
                     AIAVTCLSGLIFSGNLALSEANRPQLLQRTFDRSYIVKYLGIDAYTIYDGIKTGMTSS
                     VRAHASSNGIDEVLDYTKKHYAEPNPETFGIAKGKNVIVLHLESFQQFLINMKVDGQE
                     VTPFLNSIFQNQATISFDNFFHEVGQGKTSDAENMLETGTFGLPQGSLFTELGSDNVF
                     QAAPAILGQKQGYTSAVFHGNVASFWNRDHVYKNLGYDNFFDRSYFDESDETLGYGIL
                     DKDLFRESAQYLEHLQQPFYTKFLSVTNHTPYYTDDKNFDFPSLNTGNSTVDNYVRTA
                     HYLDQSLEQFFTYLKKSGIYQNSIFVIYGDHFGISNTDNKDLASALGKDPDTWDEFDN
                     AQMQRVPLMIHMPGYTKGTVNHEYGGEIDVLPTLLHLLGIDDKDYLHFGTDLLSSQHD
                     QVVAFRNGNFVTPKYTVLGGKAYNNQTGEIIDTKAAGIDEEISKDQEKVKTALSLSDK
                     LNQENLLRFYVPNGFETIDPKKYDYKNEAERNEAIEKQKGEHSTSVFSKNGNKTTTNL
                     YPVTRTDVEQQKNNQ"
     gene            complement(23196..23819)
                     /locus_tag="ACG842_001170"
     CDS             complement(23196..23819)
                     /locus_tag="ACG842_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0247729.1"
                     /translation="MRKKRPFLLFWLFGFFLLLILSAAFSGKMTADLAQKETEKIVAS
                     KESVDNFKKEFSTYWWAEYKITANYEKGELDIYLPKGKEENQFINIDDVLSAISLRQY
                     QFKDKHLTVLLLTHDGDKVARLKNGKVITAYSEKVDKKVVADRLAEWKKTNEQVKKTT
                     VSSQKNESTGTSPSDSGSGQTITGSEENQASIITNESSQGQYQSYGQ"
     gene            complement(23978..25375)
                     /locus_tag="ACG842_001171"
     CDS             complement(23978..25375)
                     /locus_tag="ACG842_001171"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFQ0247730.1"
                     /translation="MKKYQFPANFWWGSAASGPQTEGRISGDGKGENIFDYWYKKEPQ
                     KFFDQVGPEKTSQVYTKYQEDVQLMKQTGHNSFRTSIQWSRLIPDESGTINEEAVRFY
                     HRYFDELIENGIEPFVNLYHFDMPMYLQEKGGWLNRETVEAYEKYAKSCFELFGSKVK
                     KWFTHNEPIVPVEAGYLYRWHYPEESDMKKAIQVGYHEALASALAIKSYHEMEQGGVI
                     GIILNLTPSYPRDESNPEDVKAAQIADAFFNRSFLDPAVKGEFPSELIEIVKELDLMP
                     VIHAEDLTVIKENTVDLLGINYYQPRRVKAKETPIDTKNGPMPEDYFDNYEMPGRKMN
                     PYRGWEIYEQGIYDILINVRDHYGNIDCFISENGMGVENEARFIKEDGMIHDDYRIEF
                     VQSHLKYVHQAIQEGANCLGYHMWTCMDNWSWTNAYKNRYGFISVDLAQDGKRTIKKS
                     GHWFKDVSDKNGFEA"
     gene            complement(25407..26378)
                     /locus_tag="ACG842_001172"
     CDS             complement(25407..26378)
                     /locus_tag="ACG842_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFQ0247731.1"
                     /translation="MASIRDVAKMAGVSVGTVSRYLNGQQLKEKNMKKIAEAISALDY
                     KENIIAKGLKNNRSFSIGLLINGISSRFGSEVVSGIERVAEENGYSLLLSGFADQPEK
                     IEQKIEYLMKHVIDGLIVFLSEEEWSGFEMLTKMSIPVIALNCPEGPAGIDTILVNDR
                     ESVAKVIAHHGEQGHKKIGFIAATQTDYVARERLAGAKEAVQNDPSIQLEVFTGDYSR
                     KSGYYGAKALLEKGATAIFVSNYNMSIGAIECFNQEGVRIGKDIAFSHYDYLDKNDLS
                     ILPKITITPPTDKIGERAAEQLLKRIKKEEEENEKVIVMENIIHGIN"
     gene            26752..27177
                     /locus_tag="ACG842_001173"
     CDS             26752..27177
                     /locus_tag="ACG842_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0247732.1"
                     /translation="MKKLIVLFILSAGLLTLTGCDLFGGSDQSSDETTDSSVLKAEKA
                     DTTNSVYTLKVRKDMDLSAGQLTFTDKKMEWTRTYQTQGTDSSESSEEKTVLTDIKIT
                     TKDDEYIISGKENKKETTVIFTKIGNYRLKDEDGNIYSL"
BASE COUNT         7952 a         5726 c         4809 g         9145 t
ORIGIN      
        1 ttgtcataac ctaaaaaagg cactgctaag caaaagcagt gccttgaaat attttattta
       61 aataacacaa aaacataatt actcataaga aaccaattct gtattatcta ccatgaatgt
      121 aaagaacccc tcattaggca tcggatgaaa ataagcattg atcactctta tcaaggcatt
      181 atggcaaact aacagataga cattttcttc gtcttcgatg cattctttca ataaaggaac
      241 gatccgtgca tacacatcta agactgactc cccatttggg aatcgtacag cgaacgctaa
      301 acgtgctttt tgaaagttct catctttgct tggcagtcca tcataatccc caaaatccat
      361 ttctaccaat cgttcatcaa cggtcagcgg caagctgaga cgatcagcaa ctgcttgtgc
      421 tgtatctctt gctcgttgaa gaggggaatg gatgattttc gtgattggaa caggcaaatc
      481 tactaccttt tctgctagtt tctgtgcctg catctgtcct ttttcagtca gcggcacatc
      541 tgcccgtccg caaactaatc cttgtgcgtt ccaagttgtt tctccgtgtc tcgttacata
      601 aagcatgtga ttctctcctt cgttggcaaa gttcccctta accatacctt gttgttcttg
      661 agattacaag ggttagattc tctcaagaga aataatctca gtagatactt tatccaggaa
      721 ggtttgatca tgttccacta gtaacatggc aggtttcacg ttcaagatca gttgttctaa
      781 ttgttcttga ttgaagacat ccaaataatt caatggttca tcccatgtat atagttcagc
      841 tggctgtgat aaagatttag ccaattccac ttttttccgt tggcccatac tcatctgctc
      901 gatcttgtta tgaaaaacat ctctttccat cccaagcttt cggaggttgt tcaaaaatgc
      961 ttggtagtct acttggtttt tctctgcaaa ttccgccaac gttcctcgat tgtcttcata
     1021 attttggctt gcataactaa tgctcagatg ttttggctgg tatttttctc ctataacctt
     1081 gccgttgaat gcccccagaa gataatggat aatggatgac tttcctgcac cgttaggacc
     1141 tgaaatcgcc acccgctgat gaggctcgat tgtaaaatga attggctcga ataacagatt
     1201 ttcataccct aattgaagat cttctacgct taaaagtcgc ttatggtgag acgcttggct
     1261 attcatcgtc aaagaatcga tatattcgat atcttttaat agtttttctt tctcgctgat
     1321 ctgggtctcc atccgattca cgattgcttt ggatcgtttc atcgtccgtg cagcatcagc
     1381 acccactgct cctttattca ctcgtctaga ttcagtatcg atgaatccga cttgtttctt
     1441 tgttttatct ccttctcggg aacgagacca ttcggctttt tcagctgctg ttttctttag
     1501 cctactgact tcttttttca atttttcatt ttgagccatt tcaaattcat cacgaagttt
     1561 tttctgttct tcatagatag agaaattccc ttgataaagt tccagttgac ttttttcgat
     1621 tgctaaaaca tggtccacta cttcatcgat aaatccccgg tcatggctga ccacgatgaa
     1681 gccttgtttt ttctttttca aataagccgc tacttgtttt ctaccagaga tatccaaatg
     1741 attcgttggt tcatcgatta acgggaaatg agtgtcatcc acaaataaaa gtgccagtag
     1801 gacttttgtc ttctctcccc ccgatagtgt gctgaattct ctccataaga tttcaggatc
     1861 tgtttgcatc aattggagct ctctttcgat ttcccatatc tcaaaatccg taatatcatt
     1921 taacacgtaa taggttaaac gttctttatc ttttgtctgt tgcgggaaat aggcaaattc
     1981 ttgctgatgg attacttgcc cttgataagg tagtttgttt tgtagaatat tcagtaaagt
     2041 tgtctttcct cgaccgtttc gtccgataag tcctagtttc cattgtgtgt caaaatttag
     2101 atttgcttgt tcaaataata atgtgccttg gctgtcgtag ccgaatgtca gattttttat
     2161 ttcgatttta gacattttcc atcacctcgt aaaaaaagac cagtcctatt ttcagaactg
     2221 gctaaagagg atgcaagggt tggatacaca caaaaagacc catacatcga aaaaaccaag
     2281 ccaatcccga aaatctgcac actgcttcct tgtctccaca aggtacagtc tgaatgcagc
     2341 tttcatcaat cgacttaatt tttcaatgca cggatccctc actttgtcct atatttgaaa
     2401 ttacgatagc ataaagaaaa tgattttgtc aactacttcg ggaggatggt gaaaaatctg
     2461 ctaagtgtct gtcctaagat ttttgttttt tctaatgttt gcctgtgttc cattgcttct
     2521 tctaaaccta ttggacccaa atgaatactt aagacaattg ggagaaaatc agaaagtcct
     2581 tctagctctt ggtctctact tccgcaaata gcgattattg gttttcgata tttttttgca
     2641 agcactgcta caccgtatgg aacttttccg tggatcgttt gctgatcgat ccgcccttct
     2701 cctgtaatga tgacatctgc ttgttcgatc tttttttcca atccagtgat ttggctgact
     2761 aaatcgaaac cagaaaccat cttcgctccc aacaaaaaaa gggctccacc aataccgcct
     2821 gctgctccac ttcctggaat atcatctaaa tccacctccc agaaatcttt tatttgctga
     2881 gcaatctttc ctgcttgttt atctaagaaa gctagcgtcg tatctattcc tcctttttgt
     2941 cttccaaatt gccaagcagc tccttgttga cctgtatatg gattggtaac atctgcagcg
     3001 ataatcaatt ttactttaga aacaagaacg tttcttcgat caaaatgaag ttttttagta
     3061 gataataaag gatttccttc tgtgtaccct tctaaagaag cacccaagga ttggagtaat
     3121 cctagaccac catcgctaca cccgcttcct cctaaagtga caataatttg ttctacttct
     3181 tgaagcagtg catctttaat aagttctcct aaggcataac tggaagcaag tcggatagtt
     3241 tgatccgttg gtttatcaag tagatctaac cccaatgctt gtgcagattc gatgactgcg
     3301 gtgttttttc cttgccaagt tgtcaataaa tagctgattt ttcgctgacg gaataagaga
     3361 tccatcccat caatggtacg ccatgttccc ctgagcgcgt aatagattgc ttctgcagta
     3421 ttttctccgc catctgcaat cggcacattg atttttcttc ctttacgatc ttcccagcca
     3481 tttagaactg cctgatttag ttcaaatgaa gaagcacttc ctttaaatga atcaattgcc
     3541 gccaaaatat ccatctgtat ccctcacttt cttcttaagt aagacacttt ttttcatttt
     3601 tttcaaacaa aacaatgcta ttttcttcca tttttctata tactaaaagg gaactcattg
     3661 aataaaggag agaaaaaaca tgaaaatcgc aatagctggg gccggagcaa tgggcagccg
     3721 aatcggattg atgctacatc aaagcggaaa tgaggtcctc ttgatcgatc gttggcctgc
     3781 tcacattgaa gccattcgta caaatgggtt gattgctgac tttaacggta aagaagtggt
     3841 cgcaaaactg ccaatctatt caccagaaga aatcatcgaa agcaacgaac acgtggactt
     3901 gatcgtagca ttaacaaaag ccaatcaatt agatgatatg ttttgttcga tccaatccat
     3961 tattactgac aatacttatg ttttgtgttt actaaatgga ttaggtcatg aagatgtact
     4021 ggaaaaatat gtgccaaaga aaaatatctt atttgggatc acgatgtgga cagctggtct
     4081 agctggtccc ggaaaagtca cattgttagg tgatggagaa atcgaactag agaacttgga
     4141 accagaaggc gaagcattta caaaaaaagt tgtagaagtc tttcaagaag caaatttaaa
     4201 tccgatctat agccataacg ttcgttattc catttggcga aaagcttgtg taaatggaac
     4261 attgaatggt ttatgcacga ttttggattg taatattgct gaactaggcg cgcaaaaagc
     4321 ggcagagagc atggttcgaa caatcgtatc tgaatttgct tctatcgcag caaaagaagg
     4381 aatcatacta gatcaagaag aagtgtacca acatattgct tctacttatg atcctgacaa
     4441 catcgggttg cattacccat ctatgtacca agacctgatc aaaaaccatc gtttgactga
     4501 aatcgactac atcaatggtg ccatttggcg aaaaggacaa aaatatgata ttgctacacc
     4561 atattgtgcc tttttgaccc aattagtcca tgcaaaagaa ggaatccttg gcgcagaata
     4621 aaaggctctc tgatgagaat cagctgaatc agcagctggt tctctttttt taagcccaat
     4681 aaaagatcaa aaagaaaaca caactctcat gatgccataa gaattgtgct tgtttgtttc
     4741 agtacataat ctcttcaaaa tgtaggtttt ttatttgttt tcaacagagg agaggtcata
     4801 ttcgtattgc tcataaatag tggaatcgtt agtgataaat cgttttgccc aattttctgc
     4861 atcataaaat tccaatagct ctgataggat tggcttaaat ggtgtatgca gaaattccgg
     4921 taattcatct aaatcacgtt ctcgaagcaa aaaaatattt tcaggacgat aaaaatgata
     4981 atgagcaatc gcttgattat ccgtcactag tttcttttga aaatacatgg attcaaatgt
     5041 ccgcaaagtc actccttgct gaccctcccg caacagttcc agaatcccgc gcgattcccc
     5101 tgtttttttc aggtactctt cataagtaag atatccaggt tgttcattcc ctcgatgtgg
     5161 aagaatgaaa tagttggtcg taacatctct ttttataaat tcctgcttat agctttctgc
     5221 tcgttctttt ctcccattat tcgttgcacc aaaaaataga tccgtctttg gttcgctaga
     5281 agaaaaattc atatgtttcg aataaaatga actattatgt ttcagcccaa gttcttttgc
     5341 ttccaaaaaa tcaaaatgat aaatctcatc gattgccggt tcatcttgtg cctgcttcag
     5401 catcgcctcg tgttctgctt tgaagtgatt ccaaaaaaat aacacaattt ttgcattggt
     5461 aaatgggcgc acgcgtcgag ccatctccat atcaaatcca taatctaaaa aaacgatcag
     5521 ctgataattt gccaactgtt gtctagctga tttaatgaat tccgactgta gtaaatcttt
     5581 tccggaccaa acatctaaag agcggatgga aggcaaaaaa taaagtgatt tattttttgc
     5641 aactagcagt acgtcatttt tgttcatctt ttccgcctca ctttctctgt cactttttcc
     5701 tatgatagcc tgtttaaata gtaaaaagcc agtaaaacgt ttcctatatt tgtatgaaaa
     5761 tttaacattt gtatgaaaat atgatgaaat aaatcattct tctattaaaa ttcgcctaaa
     5821 agttttctga atagttgtaa ttgtctgaaa atttgttatc attagtcatt gttaaatatt
     5881 gctatttatt cttaatataa attttgattg tgaatttaaa agaaagaggt catttatgga
     5941 agataaaaaa ttgaaaaaac aacttagctc cagacatatc accatgttag ctttaggtgg
     6001 agcaatcggt gcaggtctat ttaaaggaag tggcgaagcg attgggatcg ccggcccttc
     6061 tgtattgatt gcttttttga ttggtgggct gattttatat atcgtgatga aaggtctggg
     6121 aaaaatcgtt ttaagcggtg gcgacacgca tcacggatta tctggtttga tacgccctta
     6181 tctaggtgct cattctgctg attttactga ttgggtctac tggtctatgt ggattatcaa
     6241 tatcatcgct gaagctgttg cagctgcttc ctttttgcag ttgtggttcc cgcacattcc
     6301 agcctgggta tttgttttta tgttggctgt tttaactacg atcatcaatc tttattcggt
     6361 acggttattc gcagaaacag aatattggct ggcttttgct aaaatcagtg tcattatctt
     6421 actgatcatt tttgctactt atcttgtcgg tcagcaaatg ttgggcacag gcgttttccc
     6481 tacccttcag caattgactg accatggtgg gtttactcct catggtatga agggaatcat
     6541 cagttctttg cttgtagtta tctattctta cggcggttct gaactgattg ctatcacggt
     6601 aagcgaagca gatgatccta aacgtgcgat tcctaaagca atcaaaggtg tcatgggaag
     6661 aatcatttcc ttttacatca ttcctttgtt tttgcttttg attctttttc cttggaatac
     6721 tttagcaggc acgaatgtca gtccttttgt gatggtattt gaaaaaatga atatcccatt
     6781 tgcagcggat atcgtcaatt ttgtgattgt ccttgctttg ttctcatcta tcaattcggg
     6841 tgtgtatgca tcttcaagaa ttttgttctt ccgtttgaag gatcgaaaag gttcctctag
     6901 aaaactagct gttttaaaca agcatcaagt accgcaacgt gctgttttat tttgtaccag
     6961 tgttctttat cttggtgtag ctctttctta ttttgtcggt gacaaattat ttggctatct
     7021 ggcaggttcc ctttcgtata cagtattatt gatctggatc attatcagtg cggcatcctt
     7081 tgtactggca ctaagaaaag gaacattatg ggataaagga atcagctttt ttgcattagc
     7141 tgtcttagga ttgattttcc ttggtatcct gttcacgaac tccattgggg taactgtatt
     7201 gactggcctg ctttacctgt tcatttattt cagttatcaa aaaaagaatg atgcgttcgt
     7261 attgacgaat gaatgaaatc gatagaaaat tttattttat ttatgtggaa tagatggagc
     7321 tattgaagaa aggagttgtg acaaaagtct tgttgcaact cctttttccg aataaacagt
     7381 ctcatgacct cttggtaaac ggtgttcaaa aaccctctgt gcaaaataat tgattgttct
     7441 ccatgctcat gacatgatag aagaaaggaa tgaaaaaaag gagtttgttt taaatgaaaa
     7501 agtattggaa gtttttgttg atgatcggtg tttcaacggt agttatgttt ggtatgatgt
     7561 acttgaatgt ctatgcgctc gatcatatct tcttcagtcg tacacgagta ttcatggcgt
     7621 tgatgatggg tgcagtcatg gcagtcatca tgctgttgtt catgtggaaa atgtatgaaa
     7681 acaaaaagat aaatgttatg atcttagccg ctagtcttct tgtttttgcc ggttccttgt
     7741 ttatggtacg aagccaaact actgtcaacg acacagcttg gatgaaggcc atgattcctc
     7801 atcattctat cgctatcctg gcaagtaaac gagctgattt aaaagatcct gaagtaaaaa
     7861 aattagcgga agaaatcatt caggcacaag aagaagagat cgacaaaatg aaaaaaatga
     7921 tcaaagaaat ggaatgacca ttatccgtcc aaataaaaaa agaggatgtt ccagaagtta
     7981 tcagcttcaa gaaataagaa gaagctactt ctggaacgcc gtgaataagg ggttgtgaaa
     8041 aagctgccct gcatcaagta ataagaacag ccaaacaaaa atagcttctc ctatttttcg
     8101 tttggttagg cttattaccg cagatgcaag cttttgaaca ccgtttatta agaggttgta
     8161 aaatcagcgt tctgacctga gtattagaga gacaaatgga aaaatagttc ctcatatttt
     8221 gccatttttt gagctaatac cgagggtcag ctgattgaac accgtttatc gagaggttgt
     8281 gaaaaagtcg cttagtttcg cgtattaaga aaaattttgt gaaatggctt tccacatttt
     8341 tgtataaatt tggcttaata ccgagaaact ggcttttgaa taccgtttat tcgtaaaagg
     8401 gattgtgaca aaagtcttgt cacaatccct ttttatgttt ttattgttct aattgcgctg
     8461 tcttgcttac tgcttcttgc ggtttcacaa atttttttga gattccccaa cctgtgaagg
     8521 cactgaccat cgaaaacaca ggagaaagca aactgaaaaa tacgaatggc aagtaagtga
     8581 gtgtaggaat accaaatgta cttgctacga aacttccggc aattccccaa ggcactaagt
     8641 agttgattac tgttccacca tcttctagcg ttctacctaa tacagttgga tcaaatcctg
     8701 ctttttcata agtttcttta aatgcattac caggcagaat aacagatagg aattgttcac
     8761 caataaaaat atttactccg atacttgtca tcaatgttgt aaaaatcaaa tttggtgtgt
     8821 tcgtcatctt tcttgatact cgttccatca cggttgagat caagccaaat tcgatcaaca
     8881 atccacctaa tgacaaagtt agaataatca atgacacagt aggcatcata ctttcgattc
     8941 ctccgcggct aagcagtgaa tcgatttgtt ggtttccagt atttgaaaca aaccctgatt
     9001 cgatgacccc agccaacttc gttaaggaag tttgtgggtt ttgaataaaa atcattccta
     9061 ctgctacagc gatattcaac aaaatcgtta aaattgccgg cacctttttc catgcacaca
     9121 gtaacattaa aaataaagga atcagtgacc agattgaaat agtaaagttt gcttctagaa
     9181 catcagtcac ttcacgaata gatgaaagac ttgcggcttt acttgtgtgt cctaaaatca
     9241 tgaataaaaa gaaagaaaca acaaatgccg ggacagttga ccacatcata tgcttgatgt
     9301 gtttgaacaa atcggcatca acaatagctg ctgctagatt tgttgattcg gataatggag
     9361 acatcttatc tccaaaaacg gcacctgaaa caatagctcc aacaaccagc gctggattta
     9421 agcctagtgt tgtcccaata ccgaaaaatg caatcccaat cgttgacata acagtaaagg
     9481 cactccctac agcgctacct acgattgcac acacaacaaa tacagaaggg acaaaccact
     9541 ggacactgat caatttgaac ccaatgacca ttagagttgg aatgatccct gcttgtatcc
     9601 atacagcaat caatgaaccg actaaaataa aaataaagat tgggatgatt cctggtttga
     9661 tgccattgat gattccttcg tgcagccaat ctgtcgggta tctttttatc actgcataca
     9721 acatgaccat tccgatcgct gctaagatcg tgatattagg tgagaccttt aatccgacaa
     9781 cgccaatcgc aatgcctaaa acaataacca ttaatacaag tgttgcttct aaaaaacttg
     9841 gtgttccttt ttttcccatg ataatctctc cttttttagt tgttcttatg ttctaaaaaa
     9901 gttaagatat cggattactc cgccgcaatt catccgcgat tcctccttta taaaaataaa
     9961 aagacctgtt ttaaactaag gacgaatgac tccgcggtac caccttaatt aaaaacagat
    10021 ctgtttttca ctttcaaact tttattcaaa tgtatggact ttccatgtaa ttcgtcaatt
    10081 aggcttgatc ggttcacagc gaccaccgac tttctgaaca aaacacctaa ccgctactgc
    10141 gtgaaaagtt gacttgtacg aagtttacct tccataagat catttgtcaa tgaaaaagaa
    10201 aatatttttt catttttctg aaaattattt ttataacttt cacacacaag attatttttt
    10261 actagaaagt agcgccctct tgtttttttc ttgctactat ttcttgatgt tcttgcttga
    10321 ttctttctaa tagctttggc tctttcaaca gatcaagagc tgtattggcg agtagttctg
    10381 ctgcaatcgc aatagaagca aggcctttgt cacttttagc agcagctttg aattcttctg
    10441 tgtgtccggc aatatatgtc tcagagattg ctaccgtagg ctggattgtt ggtatgactt
    10501 ggctgacatt tcctacatca gaagaaccga gacttgttct ttgttccgtt tctatttctt
    10561 catctggaac acctgcttct tttgcatgct ggaagaataa gtcatcaaaa gaaggagtaa
    10621 cgatgacatc atcaactgca ttttggaaca atccaaactc aaaagtcgtg cccgtactta
    10681 tcgcagcacc tcgtacgata ttttccactt tttcatatac tttatctaat gtttgccgat
    10741 ttcctgcacg taaatagaaa cgtccagcag cgtaatctgg aatgacgttg gcagctactc
    10801 ctccgtctgt aatgatccca tggatattga catcttttgg caattgcaag cgtagagcgt
    10861 tgattccatt aaacacttgg atcaatgctt ccaaagcatt tactcctttt tcaggtgctg
    10921 ctgctgcatg ggaagcgaca ccgtaaaatt tgatgtcgac tggatcattc gctaaagatt
    10981 ctgtcgtttt cccataacga aaagctggat ggacgcataa cgctacatcg acatcctcaa
    11041 aaaaaccttc tcgaacaaag ctgcctttag ctgatccgtt ctcgccccct tcttcccctg
    11101 gggtgccata tacacggatt tctccaccag tttcatcaat gaattgcttc actatgctgg
    11161 cggctaatac ggagtaagta ccaaacaaat tatgcccgca tgcatgacca atacctggta
    11221 aagcatcgaa ttctgccaaa aaggcaattg tgggaccggg tttattggaa atataacgtg
    11281 catcaaaggc tgttcggtgt ccagctactt ctttttctac ccaaaacctt tctttttcta
    11341 attgtttaat caacgctgct gaggaataaa actcatagtt acttacttca ggatgttcgt
    11401 ggatatctag agcaagtgct tgatattctg gtaaacgata ttggattaga tccttcaatt
    11461 ggtttgttga gattgttgtt gtcataaaaa attcctccta tgctagtcat ttttcttatt
    11521 ttctcccgaa tgtttcccat gcaggcacgc ttgatccttt tgatgttttc tcgattactt
    11581 tgactgtgtc ttctgtttga taagcgtcta ctattttttg atacgtttta ttgttttcat
    11641 cttcttttct tgcaacgatg atattcacat aaggacgtgc atggtcatcc actggttcta
    11701 agaagatggc atcagaagca ggattaaatc cagcatctac agctacaccg ctattgatca
    11761 ctgaaatgtc tacatctgac aaagcacgtg ctgtttggga agcatctaat tcagaaatgt
    11821 ctagattttt tctattctcg gtaatatcag aaacagtggg cgcttgtcct tttgccttat
    11881 ccactttgat caatccagca gtttgcaata acagaagagc tctgccgcca tttgtaacat
    11941 cattagggat tgctactttt gctccgtctt tgatatcagc tacatccttc aaagtgttag
    12001 aataaattcc cagcggtgca ttgacagtat ttccgatcga tactaaatca gttccgtgtt
    12061 ctttattgta attgtctaaa aagaattgat gttggaaaga attcaaatca atcgatccat
    12121 ctgctaaagc cacatttggt tgattgtaat ctgtaaattt cacgtattcc aaattaatgt
    12181 tctccttttt caaacggtct ttcacgttgt cccatacatc tgtatcttct ccaataatcc
    12241 ctagttttac gatagtttct tttttgtcag ttgtatctgc ttttccttgg ctacaccctg
    12301 ccaaccaaat aattgctaca aatgtaatga ctacaaaacg aataattttt ttcatttttt
    12361 ctcctccact ttcaatgtgt aattttttta atcagttgat tactgataaa ttgacttaaa
    12421 aaaactaaga ttagaatcaa taatgtagcc acaatcgtta catctgtttg gaatcgatta
    12481 taccctctcg atatagcaag attccctaat ccgcctgcac ctactgctcc agccatggta
    12541 gtcagtccga tcacgttgat gatagtcaac gccgagaccc gaatgatacc aggcaaacct
    12601 tctaacaaat acacacgtaa aatgataccg attggactgg ttcccatcgc ttctgccgct
    12661 tcgatcaccc caggatcaac ttctagcagt gcgttctcga tttgacgagc aaaaaacgga
    12721 acaactccaa aaatcaatgg cactaaagct gctttcgcac caatcgtcgt tccaactaag
    12781 aaacgagtag tcaatgctaa aagagaaagc aagataatga aagggatgga acgaatgata
    12841 ttgatcagct tatcaagtat ttcaaatagc agacggttag ctagtaatcc attagggcgt
    12901 gtcactacta acgtcacacc taagatgatt ccaatcatcc cgccaatgaa tgctgtccaa
    12961 aaagacatat atagtgtttg cttcgtcgct tcgacaaatt catccggaat caaataaaca
    13021 ttgggcaaat atgttttaaa gaaatcagtc attttatctt cctccttcga tcactttgat
    13081 acgactatct aaatcgtttt tttgtaaaat atttattttt acaccagatt tgatcaaata
    13141 atccaatgct tttttccgct gtttggtttc tccagctaaa gtaacgatca aactccctaa
    13201 cggcgtttct tgtaataact ctacattgcc atataaaata ttcgctgtta cttgatattt
    13261 gctgtacaga tgagcgatta gaggttcgtt cgtctgattt ccaatataac ttaattccac
    13321 cagccattta ttggcgatct gatcggcaaa agcagaatgg gcaatgatcg tttctaatgc
    13381 ttggtcaaga tgagtagctg ttcgaataaa atctttggtc aattcttctt caggattgct
    13441 gaagatctgg atgctgctac ctttttcaac gattcttcca tcttccataa ccgctacctt
    13501 gttgcagatt tctttgacaa cttgcatctc atgagtgatc aggacgattg ttaaacctaa
    13561 ctggcgattc aattttttta acaatgcaag gatctgaagg gtcgttttcg gatcaagtgc
    13621 actggttgct tcatcacata ataatacctt aggttcattc gccaaagctc gtgcgattgc
    13681 tactcgttgt ttttgtcccc ccgacagttg tttaggataa gcagatgctt tttcttccaa
    13741 accgacaagt tccagtagtt cattcacttt ttgacgacgt tcctgtttcg attttccaga
    13801 atactttaaa gaaaaatcaa cgttgtcaaa aatagttcgg ctttccatta gattgaaatg
    13861 ctgaaagatc ataccgatgg cctttctttc ttttcgcaat tctttgggag agagctgact
    13921 cagattcgtc tgattgacaa tgacttctcc tgctgttggt ttctgcagca aattcatcac
    13981 acgaactaac gtacttttcc ctgcacctga gtaaccgacg attccataaa catcgccttt
    14041 ttcaatcgtt aaatctacag aatctactgc ctgtatctct ttttcttttt gtttgaaaca
    14101 tacagaaaca tctcgcaatt caatcattaa aattcctcct tatctaaaaa aagcgtcctc
    14161 aagaaaatga aaatgtcatt ttcttgagga cgcttttttg cgtgttacca cctcatgttc
    14221 aagctgtgtt cacacacaac tcctcttcaa gtacgatcat actctagcaa gatatcggtc
    14281 gcagccgtca tcgccttaca tctatgctcg ggatgaattg ctcgatggcc attttccaat
    14341 agtcgttcct tgcttcttct cagctaccga agctctctgt caaaggttct tctatcgtac
    14401 ttaccatttc aatgcaaata atattcaaaa aaaaagtcct tttactaaca taaactatgt
    14461 cagtaaaagg acgacgtatc gcggtaccac cttttttcgg aagtttcctt ccctcaagcc
    14521 caacatctca aaaagagaga ttggcgttcg ttttaacggt cgaacagttc cgaatttgcc
    14581 tacttatcga caaattattc tccaaggcca ttttcagctt ttcttacttg actcttctca
    14641 ccagccagag cctctctgca aagtttccaa acttactttc cttttcaacg aaatcgatat
    14701 tttgttgttg ggattaatct acatgaggaa aactgaaaag tcaagtacga aattcaaatt
    14761 attttttcta tatttatttt tctgtttttt tatttaacat ttcaataaca gttcgaagat
    14821 cttttccttc cgcaagaagt ttcaattttt ccttttcttc gacggtataa tttcggacaa
    14881 aactggaatc aggtatcaaa acaacgccta atcctgctcg tgaagcagct gttgctccta
    14941 ctaaagaatc ttccactgca aaggctgaac tagcttctaa tcctgttcgt tccaaagctg
    15001 tcagataagg aatcggtgaa ggcttgttct ccgacacttc gtctccgaat gtaaacgtgt
    15061 ccaagtaatc agctaattta aagtgatcca agatatctgt tgctcttttc gtaacagtgg
    15121 tcgtagcaat gcctagtttc atatgacgtt cttttaagtc tgccaatatt tcttttgcat
    15181 atggttttaa gtggatggct ccgcttgtca gctgttgata aaaatattct tcacgcttcg
    15241 ctcggacttc ctgtactttt tcagggctac ctgctgcttt cactaaaata tcaaaacttt
    15301 gtttccagtt ttttccgccc caagcgtcaa cgatttcttt tgggatatgg atatcaaatt
    15361 gctgaaatcc ccaaagccag ccatcacgat acgttttttc tgtgtcaact aacaaaccat
    15421 ctaaatcaaa tattgcacct ttcatctata cattaatcct ttccaaaaca atacacatag
    15481 tttagcacga ctggtccaaa aagaacagaa gcaaagaacc agccttctcg tttataagag
    15541 ttcttggaat cgacggatat agattttctt cacagctgtc acaagcgcca gataagcgat
    15601 gactgtagca aataaccata accagtaatt gccaggaagt ggcatcaagc ctaattcagc
    15661 accaaaactt gtaaaaggaa ggacagaacc gacaataatt cctaatgtag tgataacaaa
    15721 catggcaaat gttgcgttgc tttggataaa tggtactttc ggtgtacgca gtgcatggag
    15781 cactaaggtt tgtgtccata gtgattcgac aaaccatcct gcatggaata atgcgatgaa
    15841 aatcactcgc tgtgaactat ccaatgtatg gaaacttccg cccactacag caggacaaat
    15901 aacaaaatac atcaacaagt aagtagtaat atcaaatatc gaacttgttg gaccaaagta
    15961 aaccatgaat tccccaatac tagatgcatt ccattttttt ggctcgtcca aatattcttt
    16021 atccatccga tcccatggca aggaaatgca ggaaacatca tagatcagat tcaaaaacaa
    16081 aagttgtaaa ggcagcattg gtaaaaatgg caggaacgta cttgcaatca gcacggaaaa
    16141 catattgcca aaattagaac ttgctgtagc cttgatatat ttcatgatat tcccaaaagt
    16201 ttctcgtcca gaaagaaggc ctctttccaa aatcgtcaaa tctttttcta aaaggatcac
    16261 gtctgcagat tctttcgcaa tatccacagc tgtatctaca gaaataccga tatcagccac
    16321 tttcattgca ggagcgtcat tgataccatc tcctaaaaat ccgacagtat gaccattttt
    16381 acgtaaagct gtcgtcaaac gtgctttttg ctgaggattc aattttacga atacttcatg
    16441 gtcttcagct acttgtgcta attcatgatc actcatttcc gagattttct caccggtaac
    16501 caattcattg atctctagac ctacttgacg gcacactgaa cgtgtaacta attcgttgtc
    16561 cccagtcaga acttttacag ctacaccatg ttctttcaga gctttcaacg cttgtttcgt
    16621 tgtttctttt ggtggatcaa ggaaagcaag ataaccaatc aaaaccatgt ctgactcatc
    16681 tttgactgaa aattctccaa cgacgcttgg attggtcttt tgtgcgacac caatcactcg
    16741 taagccatct tcattaagtg actgtacttt tgcaagtacg gcttcttttc gttcttttgt
    16801 taatggtgta acagtaccgc caagatcaat aaatttagaa atgttcagca tttcttcaat
    16861 cgcacctttt gtgatcattt gtgttttacc agctggatca gcaaccacaa cactcatacg
    16921 acgacgttca aaatcaaaag gaatttcatc cactttttga taattgatct tatctgtatc
    16981 taaagtttct gttgcttcat caatgatagc cacatctaac aaatttttca gccctgtttg
    17041 atagtagcta tttagatacg catggcgcaa aacacgatca tcttcatttc cttcacaatc
    17101 catatgatat tcaagaatga ttttatcttg tgtcaatgtc cccgttttat ccgtacataa
    17161 cacatcgatt gcaccaaagt tctggatggc attcaaattt ttgatcactg ttcctttttt
    17221 ggccatcaca cttgctcctt tgaccaaatt cgtcgtaacg atcattggaa gcatttcagg
    17281 tgttagacca actgctacag atagtccaaa aagaaaagct tctagccaat ccccttttgt
    17341 aagcccattg atgacgataa ctgctggagc catcaatgac ataaatttga tcagtagcat
    17401 ggatgtttta tggatgccta attcaaagct tgattgaatt ggtttttcag atagcgtttc
    17461 tgctacatga ccaaatagtg tttcatttcc aacagaaaca atgattcctt ccgcacttcc
    17521 actgatgaca ttacttccca taaagacaag attttcgtaa cttgtttcgc tttgctgatc
    17581 gtcttcacat actttcgcat gcttttctac tggatagctt tcccctgtca atgctgcttg
    17641 ggaaatgaat aaatcctttg cggacaataa acgcatatcc gctggtatca tatcccctgc
    17701 tgaaagccga acaagatctc cacagacgat ttcttccata ggaatctctg tgtactttcc
    17761 attacgtttt acagctgccg ttactttcac aagtgatttc agtttttcag ccgcttgatt
    17821 ggaacgtacg gactgaatca acgtcattgt cccgctgacg ataaccatag tgaagataat
    17881 ggctacaccg aaaagatctt tatctccagg agctgctaat acatattccg tgatgaatga
    17941 aataattcct aatgcgatca acactaatgt aaatggcgtg atgtatgctt tcaatatttc
    18001 tacaatgaat ggtgtttttt tgccatatga aatcgcattt tcaccgtaat tttctcttga
    18061 acgttcgatt tgttcttctg tcaatccatt tgctgatgca tggaatagtt caaataattc
    18121 ttgtttcgtt tttccagcaa aagctgcata gattttttgt gtatctttct ttttttccat
    18181 ctcaatctct cctttgtgtc caaaagacag ctgttctcgc tgcttttggt ctttagctat
    18241 tcatcggatc gatagagaaa aaactgaaag gggctgagaa cagatagata atagccctgg
    18301 cctttcctct agatccttgt ttttcgaatg actgtcatcc atttgttctc atctccttta
    18361 ctaagtaatt ttaattcatg acttccaatc atttttcgaa tagttatttt tccaattaga
    18421 ccacctcctt tttatttttc accgatgggg atttgcggca acaaaaaaac ctctcgccaa
    18481 atactcagcg aaaggtttga aaaagcatat taaaaaaagc ttctcagatc tcatcgttga
    18541 gttttgacac tgtacaacgt aaaaagcaga tacctgcttt tagctacctt atgaaaagcc
    18601 ttatctcgac aattcctgtt ctacccattg gcgtctctcg acgtttttgg gcagtagcct
    18661 atgtttgtat aggagcctca cctaacaaat cttattcggt tttccttcgt taatataaca
    18721 actaaaaaag aggatgtcaa atcaattttc catgtttttc atactttttt taaaatacac
    18781 acgttatatt cctgtctcac gcggttttat ttgtctcatt ttccacctcc cgctataata
    18841 gaaatatagt aggaaacgag gtgctgttat ggaaagaaca cttgttatta tcaagccaga
    18901 cggcgtgaga cgccatttag tcggaagcat catccaacgt tttgaagcaa agggactggc
    18961 cattgctgaa atgaagtttg aaactatgac tcctgaatta gcaaaagaac attatcagca
    19021 cttgactgag cgcagttttt ttgatgaact gatcgattac atgacttccg gtcctgtcgt
    19081 ttatcttgtg ctggtcggcg aggaagtgat cgatatcgtc cgtaagatgg ttggagcaac
    19141 aaaagctgcc gatgctgttc caggtacgat ccgaggagac tacgcgctgc cggggactga
    19201 aaacatcatt cacgcttctg attcccgtga tgctgcggta aaagagatcg cccgcttttt
    19261 cccgccattc gatataaaca aaccgtctga aaaagcggcg aagtgataca aattctaaac
    19321 tgctggtcta gttgctgggt tcagcaatgg ccagcagttt tttctctaag gacctccgtt
    19381 gattctttcc taacatatat tcgagcactg tgaagaattc ttggtagtct ttcgtctttt
    19441 cttcccctaa agcagcagaa atcaatcttt gataataatc tgccatcaat gtttcataga
    19501 tcactgtact tctttcttta tgtgctaagc tccgtttcat atcttcattc acactacgtg
    19561 cctttttcaa ccgaccattt tctatataaa acgaagtcag gttcattaaa aaagtaaaga
    19621 gatactgctc atgattgttc aactgtgcta atcgtcgcat tctttttttg attttcaaat
    19681 aattgttctc aatgaaccga tcactgaata tccaaggaaa atcagctaga atagatagtt
    19741 caaattgacc atagtctggt atctgcatca agtattcttc aatgattcgg cattcataac
    19801 gtaaaaattc aaattctgtc tcagatactt ctgttttttc gaccatgtga agattcaaaa
    19861 ccaaataata cagatcttct gttttcttaa aatgcctacg gttattgctg atctctttat
    19921 aaggatcaaa gctgtcttct tgtacccgat ctagcattcg tctttttttc tttaacttta
    19981 ttggttcatc cagctggttt cgcatccatt catattctgc aagtgtcatg ttcattcgct
    20041 ctaaaaatga tctcaatgtt acaaatgaca tttttttcac tgaatttgtc tcaatcccga
    20101 ctagagaagt cttaaaacag attccttccg ctaattggga ctgtgtcaac ttcttgttga
    20161 ctcgaagttt cttgatcaat tcaccatcat acataatagt tctccttttt aatttaaatt
    20221 tttccacatt caataaatag atacgttaaa aaggaaaaat acacgaaaaa aataaaaaaa
    20281 tgtttttttc ataaaaaata ccaaccttca agcattagaa tgttgtctaa ctactgaagg
    20341 ttggtttgat tgtttattga ttattttttt gctgttcaac gtctgttctt gttactggat
    20401 ataagttggt tgttgtttta ttaccatttt ttgaaaagac acttgtcgaa tgttcgcctt
    20461 tttgcttttc gattgcttca ttacgttccg cttcattttt atagtcatat tttttcggat
    20521 caatcgtctc aaatccgttt ggcacataaa accgcaagag attttcctga ttcagcttat
    20581 ctgaaagact taacgcagtc ttgacttttt cctgatcttt ggagatttcc tcatcgattc
    20641 ctgctgcttt cgtatcgatg atttctcctg tctgattatt gtaagctttt cctcctagga
    20701 cggtgtattt gggtgtcaca aagttcccat ttcgaaaagc aactacttga tcatgctgag
    20761 aagataataa gtctgtacca aaatgaagat aatctttatc atcgattccc aatagatgta
    20821 gtaatgtcgg caaaacatcg atttctccac cgtattcatg attgactgtc cctttagtat
    20881 aaccaggcat atggatcatc aatggcactc gttgcatctg tgcgttgtca aattcatccc
    20941 atgtatctgg atctttacca agagcgcttg ctaaatcttt attatctgta ttcgagatcc
    21001 caaaatgatc cccgtaaatc acaaagattg aattttggta aataccagat tttttcaaat
    21061 acgtaaaaaa ttgttccaat gattgatcca gataatgtgc cgttcgaaca taattatcta
    21121 ctgtgctatt gccagtattc aaagatggaa agtcgaaatt cttgtcatcc gtataataag
    21181 gcgtgtggtt ggttacggaa agaaatttcg tgtaaaacgg ctgttgtaga tgctcaagat
    21241 attgggcaga ttctctgaat aaatctttat ctaatatccc ataacctaac gtttcgtcgg
    21301 attcatcaaa ataagatcga tcaaagaaat tgtcataacc taagttttta tatacatgat
    21361 ctctattcca aaaactagct acattcccat ggaaaacagc gcttgtatag ccttgctttt
    21421 gtccaaggat cgctggtgcg gcttgaaaga cattgtctga cccaagttct gtgaataatg
    21481 agccttgagg taaaccaaat gtgccagttt ccaacatatt ttcagcatca cttgtctttc
    21541 cctgtcctac ttcatgaaag aaattgtcaa agctgatcgt tgcttgattc tggaaaatac
    21601 tatttaaaaa cggtgtgact tcttgtccgt ctaccttcat attaatcaaa aactgctgga
    21661 aactttctaa atggagaacg atcacatttt tccctttagc gatcccaaat gtttcgggat
    21721 ttggctctgc ataatgtttc tttgtataat ctaaaacttc atcaatccca ttactgcttg
    21781 catgcgcgcg aacactgcta gtcattcctg ttttgattcc gtcataaata gtatacgcat
    21841 cgattcctag atatttgaca atatatgaac ggtcaaatgt tcgctgcagc aattgtggac
    21901 ggttggcttc gctcagcgct agatttcctg aaaaaataag ccctgaaaga caggtaacag
    21961 cgatagccat cggtttgccg acagggttgc ttttgatagg tattttcttg accttcaacc
    22021 aaaccagtag cccaatgaac acagcgatat ccagccagta gatgacatca tggggcttca
    22081 tcaaagaaaa ggagcttcct gacaatcctt gcgacacttt cgaaaagcct aatacagatt
    22141 tgactgtaat gaaatctgtg aattcccgat aaaaaataat attcaagtac aacaaaagcg
    22201 tatttaaaat atcaatgatc aaaagaatag ctaacgctgg ctttggcttt ttaatataca
    22261 aagaaaggcc aaaaaacaat aaggtagtcg caattgggtt gatccacaag ataaagtact
    22321 ggatcgtacc ttccacacct aatgaaaaat ctgcatagta ggcaatgatc gtcttcagcc
    22381 agaaaaagaa aacagctgtc aaaacgattg ctaaacgatt ttggcacaat tttttaagtt
    22441 gtgtcacgta tatcacttct ctctattcac attttttctt attttgctcc tgaaaaatga
    22501 aaatttccta aaaaagttgt tatcttaaga tttttttatt ctttctaaag attattccgt
    22561 ccacttttca cagcataaaa ccgctgcgat gtaagcgatt aaactaatga agagataaaa
    22621 gtcttgtggt gaccgcttca ctccacggta ttcaaaagcc agtttctcgg tattcgtaca
    22681 aaaaaagagg ctgtgacaaa attcttgtcg cagctcctta tttcttgatg caggacagct
    22741 ttttcacaac ttcttcctta aacggtgttc tcctgccaac tcctcggcca caagtcacaa
    22801 tatttataaa acatgagaag ctgttttttg aaattgttcc ttgtggctca gagttaaacg
    22861 gcaggctcaa gacctcttga tacacggtgt tcaatcagct gaccctcggt attagattat
    22921 gtcaggacgc taattttaca acctcttggt aaacggtgtt caaaagcttg catctgcggt
    22981 aataagccca accaaacgaa aaatataagg agctattttt tgaaattgct ccttgtggct
    23041 cagagttaaa cggcaggctc aagacctctt gatacacggt gttcaatcag ctgaccttcg
    23101 gtattagctc aaaaaacggc aaaatatgag gaactatttt gccgtttgtt tctctaatac
    23161 tcaggtcaaa acgctgattt cacaacctct gtttttcatt gtccatagga ttgatattgt
    23221 ccttgtgatg actcattcgt aataattgag gcttgatttt cttcacttcc cgttatcgtt
    23281 tgtccactgc cagaatctga aggacttgtt cctgtcgact cgtttttttg agaagagaca
    23341 gttgttttct tgacttgttc atttgtcttt ttccattccg ctaaacgatc ggcgaccact
    23401 tttttgtcta ctttttcaga ataagcggtg atcacttttc catttttcaa acgggctact
    23461 ttgtcgccat catgagtcaa aagcaaaacg gtcaaatgct tgtctttaaa ttgatattgt
    23521 ctgagagaaa tcgcactaag tacgtcatcg atattaataa attgattttc ttcttttcct
    23581 tttggcaaat aaatgtccaa ttcgcctttt tcgtaattgg cagttatttt atactccgcc
    23641 caccaataag ttgagaattc ttttttgaaa ttatctactg attctttgct tgctacgatt
    23701 ttttctgttt ctttctgagc aagatctgct gtcatttttc ctgaaaatgc tgcggataaa
    23761 ataagcagta agaaaaaacc gaataaccaa aagagaagaa acggtctttt tttcctcatg
    23821 ctttcctcca ctcctattta attttgacta ttctattata tacaaaaaat gaaatagaaa
    23881 gacaaaaaag ttgaaaaaga tcgtaaataa ttgaacttct ttttcacaaa atggtaagaa
    23941 tggagaattt cacgcatttg ctctcaacta ttctttctta agcctcaaat ccgtttttat
    24001 ctgatacgtc tttgaaccaa tgacccgatt ttttgattgt tctttttcca tcttgtgcta
    24061 agtctactga aataaagcca tatcgattct tatatgcatt ggtccacgac cagttatcca
    24121 tacatgtcca catatgatag cctaaacaat tggcaccttc ttgaatggct tgatggacat
    24181 atttcagatg agattggaca aattcgatcc gataatcatc gtggatcatg ccatcttctt
    24241 tgataaatcg tgcttcgttt tctacaccca taccgttttc agaaataaag cagtcgatat
    24301 ttccataatg atctcgtaca ttgatcaaaa tatcatagat tccttgttca tagatctccc
    24361 atccgcgata aggattcatt ttacgaccag gcatttcata attgtcgaaa taatcctcag
    24421 gcattggacc gtttttagta tcgattggtg tttcttttgc ttttacccgg cgtggctggt
    24481 aataattgat acctaaaaga tctactgtat tttctttgat aaccgtcaaa tcctctgcat
    24541 ggatcacagg catgagatca agttctttta ctatctcaat caattcactt ggaaactcgc
    24601 cttttacagc tggatcaagg aatgaacgat tgaagaacgc atctgctatt tgcgctgctt
    24661 taacatcttc agggtttgat tcgtctcttg gatagcttgg tgtcagattc aagatgatac
    24721 caataacacc accttgctcc atttcatggt aagatttgat ggctaacgct gaagccaacg
    24781 cttcatgata gccaacttgg atcgcttttt tcatatctga ctcttctgga taatgccatc
    24841 gatataaata acctgcttcg actggaacga ttggttcatt atgagtaaac cattttttta
    24901 ctttcgaacc aaacaattca aaacaacttt tggcatattt ttcataggct tcaaccgttt
    24961 ctcggttcaa ccatccgcct ttttcttgca gatacatcgg catatcgaaa tgatacagat
    25021 tcacaaaggg ttcgataccg ttttcaatca actcatcaaa ataacgatga tagaaacgaa
    25081 cggcttcttc gttgattgtt ccagattcat caggtatcaa acggctccat tggatagatg
    25141 ttcgaaatga attatgccca gtctgtttca tcaactgtac atcctcttga tacttcgtat
    25201 aaacttgcga ggttttttca ggtcctactt gatcaaagaa cttttgcggt tcttttttgt
    25261 accaataatc aaaaatgttc tctccttttc catcgcctga aatccttcct tctgtttgtg
    25321 ggccacttgc tgcagaaccc caccaaaaat ttgctggaaa ttgatatttt ttcatcaaag
    25381 cacctctttt tcttttttag ttcgttttag ttgattccat gtatgatatt ttccatcaca
    25441 atcacttttt cattttcttc ctcttctttt ttgattcgtt ttaacagctg ttctgctgct
    25501 cgttccccga ttttatctgt tggtggcgtg atcgtgattt tcggaaggat actcagatcg
    25561 tttttatcta aataatcata gtgactgaac gcaatatcct tcccgattct gacaccttct
    25621 tgattgaaac attcgatggc accaatcgac atattgtaat tgctgacaaa aatagcagtt
    25681 gccccttttt ctaataatgc ttttgccccg taatagccgc tttttctaga atagtctcct
    25741 gtaaagacct ctaattggat cgatggatca ttttggaccg cttctttggc tcctgctaaa
    25801 cgttctcttg ctacgtagtc tgtttgagtg gcagcaataa atccgatttt tttatgtcct
    25861 tgttcaccat gatgagcgat cacttttgct acactttctc gatcattcac taatattgta
    25921 tcaatacctg ctggtccttc tggacaattc aatgcgataa ctggaatact catttttgtg
    25981 agcatttcaa atccagacca ctcttcttcg gataagaaga caatcagccc atcgatcaca
    26041 tgcttcatca ggtattcaat tttttgttca attttttctg gctgatcggc aaacccgctt
    26101 aataataagc tatacccatt ttcttcagct actctttcga tccctgagac aacttccgac
    26161 ccaaaacggc tggaaatccc gtttatcaaa agtccaatag agaagcttcg attattcttc
    26221 aaccctttag caatgatgtt ttctttatag tccaacgctg aaatcgcttc tgctattttt
    26281 ttcatgttct tttccttcag ttgctgacca ttcaagtaac gggaaactgt tcccactgaa
    26341 acaccagcca tcttagccac atcacggatc gatgccatac attctcactc ctgccagtta
    26401 catttgattt ctctttcttg ttgttcgatt atgatgaccg cattctttgc atagtgttgc
    26461 ttgtcatcaa tatccatata actaaagtct tcagtagagc aacttcctct cactattaat
    26521 tatacttaaa caagtatttg aaccggttca tttttaatat agcatttttt taaaagattg
    26581 caacactttt ttaaaacgct ttctgaaagt ggcggatttt ttgccttttt aaaattttat
    26641 tctaaaattt actctatttg tgggtgtttt gtgcgttttt ctcttccatc cgtttgaagt
    26701 tttacattcc aatggtataa tttctttatc ctatcaaatg ggggaagtat tttgaagaaa
    26761 ttaatcgtac tatttatttt gtctgcggga cttttgacat tgactggctg tgatttgttt
    26821 ggcggctctg accaatcctc ggacgaaaca accgattctt ctgtcttaaa agcggaaaaa
    26881 gcagatacca ccaacagtgt atatacgcta aaagtacgta aagatatgga tttatccgcg
    26941 ggacagttga catttacaga taaaaaaatg gaatggacac gcacgtatca gacacaagga
    27001 actgattctt ctgaatcatc tgaagaaaaa acagtgctga cagatatcaa gatcactacc
    27061 aaagatgacg aatatattat ttcaggaaaa gaaaataaaa aagaaacaac agtcatattt
    27121 acaaaaatcg gtaattaccg tttgaaggat gaggatggca acatctacag tctatagtgc
    27181 cgtctgttgg atcttctttt gtttctcccc ttcttttctg ctatagtaaa gaagcaagat
    27241 gattgtaaag gagacgaaat ggatgatcga tagtcctgat tattgtacat gtaataacaa
    27301 caaaaatatc catacagaat ttaatgacca aggaagcttg agcatatgcg acaactgtca
    27361 caaactagtc aaagattcat ttaaaacatt tgaatcttat cctgttcaaa attatgtaaa
    27421 aagtagaaga agataaacaa atccaagcaa aactcactat ggctgatttt tatgaacttc
    27481 aaactcagct tcttcctttg atcaagcttc atcagtaaaa caaacagtgg atcatttcag
    27541 tttacaaatc aaagagtaaa gatttattta tttcaaatat ttgaatataa aataaccaaa
    27601 cgatagaaag gcttattcta catcacgtag ta
//
LOCUS       DAZKLM010000030        27262 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3764
            SAMEA115706913-rid22582563.denovo.030, whole genome shotgun
            sequence.
ACCESSION   DAZKLM010000030 DAZKLM010000000
VERSION     DAZKLM010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706913
            Sequence Read Archive: ERR13196647
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27262)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27262)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287823.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287823.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,004
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,849
            CDSs (with protein)               :: 2,849
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 41 of 96
            Pseudo Genes (incomplete)         :: 52 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 15 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27262
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706913-rid22582563.denovo.030"
                     /isolate="VRE3764"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            269..1438
                     /locus_tag="ACG842_001174"
     CDS             269..1438
                     /locus_tag="ACG842_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HFQ0247733.1"
                     /translation="MPNFTTEIMETLINKGDLDELFRCHLELAVNSLLQAELTAFLDY
                     EKYDRAGFNSGNSRNGNYSRSFRTEYGELNLVIPRDRNGKFSQQTLPAYKRTNDSLET
                     TIIQLFQKGITMSEISELIEKMYGHHYTPQTISNMTKIVSEDIIAFKERSLESRYSVI
                     FMDATHIPLKRQTVSKEAVYIVIGIRLDGTKEVLGFSLAPTESAYVWKEILQDLKDRG
                     LKEVLLVVTDGLSGINDSIHSVYPNAQFQQCCVHISRNIAHKVRVSDRQEICSDFKLV
                     YQASSKEEANNQIRFMIDKWKKQYPRVVKLLMNPAILTFYNFPPSIRRTIYSTNLIEG
                     FNKQLKKYTKRKEQFPNEESLERFLVSQFNNYNQKFLCRVHKGFKEIHDTLESMF"
     gene            1911..2465
                     /locus_tag="ACG842_001175"
     CDS             1911..2465
                     /locus_tag="ACG842_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFQ0247734.1"
                     /translation="MSYNVQPLRTNEEIQDFQFWLRRTSNPERDSFLFLFGINNGLRM
                     SDIIGLKVGDIRGKSKPIIVEHKTGKRKPIFIDNLREEILLYTEGKEENDWLFPSRQQ
                     GRHITRDRVYQIYADIAEKLGRDDIGTHTLRKTFGYHYYKKTRDIATLMFIFNHSSQA
                     ITKRYIGITEDEIGASLRGFKLGV"
     gene            complement(2638..3075)
                     /locus_tag="ACG842_001176"
     CDS             complement(2638..3075)
                     /locus_tag="ACG842_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFQ0247735.1"
                     /translation="MFSSEENKPLYLDYVNHNLKIIIKENNLKHITLHGFRHTHCSLL
                     FESGASVKEVYVRLGHTDIKTTMDDIYTHIAKKQTEETAIRFADFMSSTEEKQGSLQK
                     VKSMVKNEKSRLSLLDSLLNVELSVFFLFRSVCVFETVLILFF"
     gene            3123..4070
                     /locus_tag="ACG842_001177"
                     /pseudo
     CDS             3123..4070
                     /locus_tag="ACG842_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016634498.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
     gene            4341..4776
                     /locus_tag="ACG842_001178"
                     /pseudo
     CDS             4341..4776
                     /locus_tag="ACG842_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345965.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit A"
     gene            4792..5625
                     /locus_tag="ACG842_001179"
     CDS             4792..5625
                     /locus_tag="ACG842_001179"
                     /inference="COORDINATES: protein motif:HMM:NF012742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFQ0247736.1"
                     /translation="MLYFSAIDNADFVFSDPFTAEVLSGIEEQAKNHGYFVLVHNVNS
                     AEDVKTIQRNWRFEGFIVVGVRVKEFLELNNVFDGPVVYIDTHLSKETLRELEQQMNR
                     LFVNTNDYQASTLAVEHLIHYGHEKIAFLSFDYIVGEPSVIQERHQAYIDTLKKYHLT
                     FKSHWIYNNSEFERIYQELAEFTAIVVTGDYLAAKLVKFLKNKNKRVMEQLSIIGFDD
                     ITFAELMDPALTTIRLNPGNKGAIAFHQLIGLAKQEEIKNQVLLLEGELISRDSVHKI
                     K"
     gene            5870..6895
                     /locus_tag="ACG842_001180"
     CDS             5870..6895
                     /locus_tag="ACG842_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFQ0247737.1"
                     /translation="MVGIRDVAKRAGVSIATVSRVLNEDPTLSVTDETKRKIAESVNF
                     YHYVKKSPVAKNLTNIALITTVSEVDELEDPYFRTIRRGIQVEAEQSKVNLKKIIRLS
                     ETPLEQDDLLKCQGVLIIGQVLSSVIEEVSAINSNVVVIDDPSIEAEVDAVYTDLTKA
                     TNQHLDRLYKKGHRNIAFIGGGRVRLDSQGNKHVSDDDYRKIAYETWMKQKGLEKYSQ
                     IYLEGWTTLDGMQAADLLLKKHAKDKLPTAIMVGNDPIAVGVYRSLQKNGLHIPEDIS
                     IVSFDNIEVAEFLTPALNTVNVNTEEIGKLAVRMVNERIKEVRNVSIKVIVSNNIIVR
                     ESEKNID"
     gene            7033..7158
                     /locus_tag="ACG842_001181"
     CDS             7033..7158
                     /locus_tag="ACG842_001181"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0247738.1"
                     /translation="MKHFKKIALTGIAVLTGTLLAACGGGDSNSKDGQVTLSYAS"
     gene            7183..8298
                     /locus_tag="ACG842_001182"
                     /pseudo
     CDS             7183..8298
                     /locus_tag="ACG842_001182"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
     gene            8381..9277
                     /locus_tag="ACG842_001183"
                     /pseudo
     CDS             8381..9277
                     /locus_tag="ACG842_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338086.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
     gene            9293..10117
                     /locus_tag="ACG842_001184"
     CDS             9293..10117
                     /locus_tag="ACG842_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002405323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0247739.1"
                     /translation="MKNKFFSNAPIHIMLILGGIAMALPFIWMILTSGKTLVESTQIP
                     PTIFPEKFQLSNYQQVLDLLPFMAFYINTFLMVVGRVIGSVLFSAMAAYACARLSFPG
                     KNILFSLVLIQMMIPGSIFITPQYLLAQKLGILNTIPALILPGVVSAFGTFLLRQFFL
                     GLPDELEEAAYLEGANIWTIFFKIMLPLAKSGLVALGIFTALFAFKDLMWPLIVNTSI
                     DKMPLAPGLANLQGQYATNFPQLMAGSLLAIWPIVVIFIVFQRKFIEGIATTGSKL"
     gene            10149..11459
                     /locus_tag="ACG842_001185"
     CDS             10149..11459
                     /locus_tag="ACG842_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005235059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase/alpha-galactosidase"
                     /protein_id="HFQ0247740.1"
                     /translation="MTKITFIGAGSTIFAKNVLGDAMLTPSLQDAHIALYDIDEKRLK
                     ESNLMLKNINKNSNENRADIVSYYDRKEALREADFVINAIQVGGYKPSTVIDFDIPKK
                     YGLRQTIGDTMGIGGIFRTLRTLPVMLDFAKEMEEVCPNAWLLNYTNPMAMLTMGVLK
                     ATSIKTVGLCHSVQVCVPELFEHLGIADQYDLNDFQWKIAGINHMAFLLEITKNGEDF
                     YPTIRQLAAEKPNPHPDSVRFELLKRFGYYITESSEHNAEYHPYFIKSKYPELIDELG
                     IPLDEYLRRCEQQINDWESMREDIVNNGELTHTRSREYASYIMDSITTGTPSVIAGNV
                     LNKGLITNLPTDVCVEVPCLVDKNGVQPTYVGELPTQLAALNRTNINVQELTVEAALT
                     LDKSKIYQAAYLDPHLSSELSLTDIKNLVDDLIEAHGDYLPEYN"
     gene            11607..12866
                     /locus_tag="ACG842_001186"
     CDS             11607..12866
                     /locus_tag="ACG842_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFQ0247741.1"
                     /translation="MKWSKKIILACIALGATISLTACGKNQEDASGKVEIEFFNQKKE
                     MTQTIQEIAKDFEAKNPDIHVKVVDVPNAGEVIKTRMLAGDVPDVINLYPQSIELKEW
                     AKAGYLEDLTEEPYLENIKNGYAQRFAIEDRVYSIPLTANVYGFYYNKTAFEEMGIQA
                     PETWAEFEKIVAEIKDQNKVPFAIAGAEGWTLNGYHQLAIATAAGGEEEANNVWRFSE
                     VNGINAESKEMQKDFNRLDLLREPKALQNNWQGAGYNDTVVTFTKGDALIMPNGSWAM
                     PMIHSQNPDFEVGTFPFPADEAGQSLTIGAGDLALSVSATSQHKEEAKRFVEYMTTPE
                     AMQKYYDVDGAPCAVEGVIENTQDSPLSGLTELAFTDRHLVWLAKDWNSENDFYTLTT
                     NYLHNGNQKAMINALNAFFNPMKADGR"
     gene            12876..13748
                     /locus_tag="ACG842_001187"
     CDS             12876..13748
                     /locus_tag="ACG842_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018363968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0247742.1"
                     /translation="MKIKEILNRYWAIIFVAIPIILQMIFFYFPMVQGAFYSLTNWTG
                     LTYNFDFVGLNNYKILFSDPKFIKSIGFTLIVTLCMIAGQIGFGILIARALNANIKGK
                     TFFRAWFFFPAVLSGVVVSLVFKQIFNYGLPKIGSMLGIEFLQTSLLGTTWGAIFAVI
                     FVLLWQGIAMPVIIFLAGFQSIPQEIIEAARMDGANDKQIFWNIEITYLLPSISMVFI
                     MALKGGLTAFDQIFALTGGGPSDATTSLGLLVYNYAFKNNQFGYANAIALVLFILIGI
                     VSLAQLKLSKKFEI"
     gene            13760..14590
                     /locus_tag="ACG842_001188"
     CDS             13760..14590
                     /locus_tag="ACG842_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0247743.1"
                     /translation="MKTSKEKNISKYLLLLLGGFMILVPLVSTLFGSFKTTPDIMNNF
                     FRFPNPATLENFRRLLADGIGHYFWNSAIITIIAVVAVTAIIPMAAYSLARKMTRGKA
                     YGLMYLFLILGIFVPFQVIMIPITVMMSKIGLANMWGLILLYLTYAVPQTLFLYVGFI
                     KLNVPISLDEAAEIDGASHFTTYFKIVFPMLKPMHATTIILNALWFWNDFMLPLLILN
                     RDSNMWTLPLFQYNYTGQYFNDYGPSFASYTVGIVTITLVYLAFQRSIIAGMSNGAVK
                     "
     gene            14630..16813
                     /locus_tag="ACG842_001189"
     CDS             14630..16813
                     /locus_tag="ACG842_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HFQ0247744.1"
                     /translation="MIIFDNHTQTFHLQNKKISYLLGIEENNYLTHLYFGKKITHYSG
                     GYRYPRTDRSFSPNNANAQDRLLSLDTLPLEFPGYGHGDFREPAHNIKLGNGSRVNDF
                     RYDSYEIQKGKPSLAGLPALYTEDDDEAETLIIQLKDAISGLTLQLFYTIYQNHSVII
                     RSTQLVNNSDETVEINRLASQSIDFPNRPLELIHLNGTWARERQMTREKIHTGIKVLD
                     SKRGSSSHQQNPFVALVDAKTDEFQGEAYGFNLVYSGNHETVIQKDPFDQTRVIMGIN
                     SFNFGWQLKTGDSFQTPEVVMVYSNEGLNTLSQTYHELYNHRLIRGNYRLKERPTLIN
                     NWEATYFDFNEEKIMNIVDDAKNLGIEMFVLDDGWFGGRENDFTSLGDWVETEGKLAC
                     GLENLAKQVHDNGMKFGIWFEPEMISEDSELFRAHPDWALGIPGRDRSISRNQYVLDF
                     SRQDVRDNIYQQMTAIFDRVPIDYVKWDMNRNMTEVYSVLLDSTMQGEVAHRYMLGLY
                     EFLEKLTTNYPTILFESCSGGGGRFDAGFLYYMPQTWTSDNTDAVARLKIQYGTSLVY
                     PISSMGAHVSAVPNHQTGRETSLTIRGNAAMAGVFGYELDLTQLTSEEKAEIKEQVAF
                     YKKHRQLLQYGTFYRLESPFEGNDTAWMFVSKDQTEAMVFYFRVLAEAAHPLVTLKLA
                     GLAEEATYQMEDVTFAGDELMNLGFYIDSELHGDYATQRFYLKQI"
     gene            16874..18094
                     /locus_tag="ACG842_001190"
     CDS             16874..18094
                     /locus_tag="ACG842_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HFQ0247745.1"
                     /translation="MFYLHAFTKKQPDLNWENEEVREEIYAMINYWLDKGLGGFRIDA
                     ILNIKKKLEYGIFESDGEDGLAFIGPWILNQSGIEVWLKEMRERTFKPHNSMTVAEAD
                     VPNERLDEYIGEDGYYSMVFDFSYTDIDVPETGEWFKDSQWTWTDMRTNIFTNQLVTQ
                     DKGWGALYLENHDQPRSINKYIPEEWINDYSKKMLATLFMLLRGTPFIYQGQELGMTN
                     IEMASLEDFDDVATHSQYKRALEYGLAPEQALKAVNKRSRDNSRTPMQWNTQKNARFS
                     TSDNVWLKVNPNYPELNAEAQLTNQQSVFNYYHQLIKLRKNSVYKDVLIYGQFLPVKD
                     KNEHILLYERQLEDKIVLVILNVTATEQEYYVDEQYQQVLINNYEDVLLDQSRKLRLR
                     PFESIVLANYGGQL"
     gene            18091..19551
                     /gene="gtfA"
                     /locus_tag="ACG842_001191"
     CDS             18091..19551
                     /gene="gtfA"
                     /locus_tag="ACG842_001191"
                     /EC_number="2.4.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004645 - 1,4-alpha-oligoglucan
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose phosphorylase"
                     /protein_id="HFQ0247746.1"
                     /translation="MKIKNEAMLITYADSLGKNMGELKQVLDKHLQGVIGGVHLLPFF
                     PSTGDRGFAPSDYTTVDPALGTWEDVEALGEDYYLMFDFMINHISRESKFFQDFKKNH
                     EQSPYKEMFIRIHEFFPENRPTQEDIDLIYKRKDKAPFQTVEFADGTTEEVWNTFGEE
                     QIDLDVTKEVVKEFIRETIKDMANHGCSLIRLDAFAYAIKKLDTNDFFVEPDIWELLD
                     EVRQEAAKYDVELLPEIHEHYSIQMKIANHDYYIYDFALPMVTLYSLYSGKSERLANW
                     LKMSPMKQFTTLDTHDGIGVVDARDLLTDEELDYTSEELYKVGANVKKVYSSANYNNL
                     DIYQINSTYYSALGDNDRSYLLARAIQVFAPGIPQIYYVGLLAGKNDIELLEATKEGR
                     NINRHYYDLAEIEEEVQRPVIQQLFALLKFRNESAAFDLDGTIEVATPSESEIVITRK
                     NKNGSDSATLTANLDTKEFIIESKDIKIFTSVALKF"
     gene            19628..20734
                     /locus_tag="ACG842_001192"
                     /pseudo
     CDS             19628..20734
                     /locus_tag="ACG842_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175341.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
     gene            21056..21265
                     /locus_tag="ACG842_001193"
     CDS             21056..21265
                     /locus_tag="ACG842_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HFQ0247747.1"
                     /translation="MVVITKNELMKLGYGKYQSERIIRQAKAIMVSKSYTYYENRRLG
                     LVPVESVEEVLGMKIEVEEDFQNHG"
     gene            21258..21407
                     /locus_tag="ACG842_001194"
     CDS             21258..21407
                     /locus_tag="ACG842_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HFQ0247748.1"
                     /translation="MVNFKKDKNGKYHFTASLGFDKRTGKRVQKNVEGLKQLKKKKSV
                     YRTCF"
     gene            21561..22226
                     /locus_tag="ACG842_001195"
     CDS             21561..22226
                     /locus_tag="ACG842_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFQ0247749.1"
                     /translation="MELREIRPIDIQKWQNNLLSKYKNSYVRNMYGIFQMSLDRAVVL
                     GLLEKNPAKVIGNVKKRKTTVDFWTKEEFTKVISTFYIDDLYQHFGFICMWLLFMTVM
                     RLGEATALTWKDIDFENKNLRVNKTLYYKRAKTYEFVEPETASSIRTIELDDNTITFL
                     KAWKERQVSIGGMSFVLSYNGTPTQKYTIRHIMRRHATIVGVHIIRIHGLRHSHASLL
                     ISQ"
     gene            complement(22510..23595)
                     /gene="xerS"
                     /locus_tag="ACG842_001196"
     CDS             complement(22510..23595)
                     /gene="xerS"
                     /locus_tag="ACG842_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerS"
                     /protein_id="HFQ0247750.1"
                     /translation="MKNTEIYQKIDTIILHMPDYIKDFVQDREDKDQSPRTTLEYLKN
                     YKLFYEWLLSESIVPDTSITSIRQLTAYDLNLYKSHLKRRTKENVKKETTKTKLKDNN
                     NLGLSTSTINRNITALKVLFKYLSKSSNNPLGKPYLDNNPMDQVATITDKTTLAARAN
                     AIEKKLFLDKDTQNYLDYIANEYKNTLSKRALIYYQRDVERDLAINALILGSGLRLSE
                     VININLDDLSLDKNNVVVTRKGNKRDAVNIAAFAMEYLANYLAIRKERYKVTENEKAL
                     FLAIYQGEAKRISGIAIERMVAKYSKGFRIQVSPHKLRHTLATRLYQQTNSLVLTAQQ
                     LGHSSTNTTTLYTHIDNAATIDALNAL"
     gene            23929..24162
                     /locus_tag="ACG842_001197"
     CDS             23929..24162
                     /locus_tag="ACG842_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module antitoxin"
                     /protein_id="HFQ0247751.1"
                     /translation="MAIKERKFREVGNSVVLTISKEFLKENGLNPGDTVFLDEEKLRA
                     AITKKQALSDEIDLAISQALTEYDTTFRELVDK"
     gene            24159..24566
                     /locus_tag="ACG842_001198"
     CDS             24159..24566
                     /locus_tag="ACG842_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HFQ0247752.1"
                     /translation="MSIHYLEAKDIIKMNVIQIKKYSPNEPLGVKDANALEMCVGQLR
                     ASAFGEEVYPSIYEKAAILLIQLIKKHPFHNANKRTAFLATFVFLKINGQLLAIDQKD
                     AVNLAVYIATYDKDFDQLKYEVINTIKTHTQPI"
     gene            complement(25703..26221)
                     /locus_tag="ACG842_001199"
     CDS             complement(25703..26221)
                     /locus_tag="ACG842_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013852041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HFQ0247753.1"
                     /translation="MRTYKNKEELKKEINRTYTKYILEFETIPEELKDKRVEEVDRTP
                     AENLAYQVGWTNLILKWENDERNGLSVKTPSDQFKWNQLGELYKWFTNTYAHLPLKEL
                     EEILNRNIDDINMMIDSMSDEDLFTAHKRKWADEATKTAVWEVYKFIHVNTVAPFGTF
                     RTKIRKWKRLAL"
     gene            26588..26836
                     /locus_tag="ACG842_001200"
     CDS             26588..26836
                     /locus_tag="ACG842_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0247754.1"
                     /translation="MVKLKMLYIGLQHRSFFDCALGKKYYEVKIDDDTFLTAHQMKQL
                     QKRNDQLEENLILKKRLPYSRLARRTIKGQDLPEDHPF"
BASE COUNT         9325 a         4140 c         5035 g         8762 t
ORIGIN      
        1 gtagtataat ttaaaggtaa taagttattc ttttattact atgtttgggt tttatagcaa
       61 agaaagttta ataagagttt ggacctagca aacaatatta atgaggtcat taaatcttat
      121 tatctttagt gatataacga aatatctaca cagacaaaat gatagcgtaa aatattttgt
      181 gtaaatagat atttagggta gaaaaaaaga aagtccattc tgtaaaatta aagttaccac
      241 aaccgaaatt ttataggagg actttctcat gcccaatttt actacagaaa ttatggaaac
      301 actaatcaat aaaggtgatt tagatgaatt atttcgatgt catttagaat tagctgttaa
      361 ttcattattg caggctgaat taacagcttt tcttgattat gaaaaatatg atcgtgctgg
      421 gttcaattca ggtaattccc gtaatgggaa ttattcacgt tcattcagaa cagagtatgg
      481 agaattaaat ttggtaattc ctagagatag aaatggtaag ttttctcaac aaactttacc
      541 tgcttataaa agaaccaatg actctttaga aaccactatt attcagctat ttcaaaaagg
      601 aattactatg tctgaaatct ctgaattgat tgaaaaaatg tacgggcatc actatacgcc
      661 acaaaccatt tcaaatatga ctaaaatagt ttctgaagat attattgcct ttaaagaaag
      721 gtccttagaa tccagatact ctgtcatttt tatggatgct actcacattc ctttaaaaag
      781 acaaactgtt tcaaaagaag cagtttatat tgtaattggc attcgattag atggaaccaa
      841 agaggttctt gggtttagtc ttgctccaac tgaatctgct tatgtttgga aagagattct
      901 tcaagaccta aaagaccgtg gtttaaaaga ggttttatta gtcgtaactg atggtttaag
      961 tggcattaac gatagtattc atagtgttta tccaaatgct caatttcaac aatgttgtgt
     1021 ccatatatct agaaatatcg ctcataaggt tcgtgttagt gatcgacaag aaatctgtag
     1081 cgatttcaaa ttagtttatc aagcaagttc aaaagaagaa gcaaataatc aaataaggtt
     1141 tatgatagat aaatggaaaa agcagtatcc acgagtggtt aaattgctca tgaatcctgc
     1201 tatattaacc ttttataatt tccctccgtc aatcagaaga actatttatt caactaattt
     1261 gattgaaggt tttaacaaac aattaaagaa atataccaaa agaaaagagc aatttcctaa
     1321 tgaagaatct ctggagagat ttctagtatc tcaattcaac aactataatc aaaagtttct
     1381 atgccgagtt cataaaggtt ttaaagaaat acatgataca ttagaatcaa tgttttaact
     1441 tgatcaacag aatgggcttt tccatttaca caaaattatt gacgctcccg acaaaattat
     1501 gatcttgcgt aaagttataa aatattaata aaaatatttt attaatgaaa agcttggtag
     1561 cacggttatt catatgctaa tttatttcac tttatagtaa ggggagttct tagtatattg
     1621 ttcaaactta aatttcatat aatttaatta tgtaaattag ctattctaag aacttctacg
     1681 gataactgcc cgtctaaaat ttaaataaga gcttagaatc ttcatataag taaggttaat
     1741 ttttttgtac aaaaaagtcg gaatattctg agttactgaa atgatataaa atcataaact
     1801 aaatcccttg attttattga ctttcaaaag tttatatata taagttaatg attatggtaa
     1861 aatatataca taatcattaa ctcagaatat ttttaagaag ggaaacaata gtgagctata
     1921 atgtacaacc gttaaggaca aatgaagaga tacaagattt tcagttttgg ctaagaagaa
     1981 caagtaatcc ggaaagagat agttttttat ttttgtttgg aatcaataat gggttacgta
     2041 tgtcagatat tataggatta aaagttggag atatacgagg caaaagtaag cctattattg
     2101 ttgaacataa aacaggtaag cgaaaaccga tttttataga taatttgaga gaagaaattt
     2161 tattatatac tgaaggtaaa gaagaaaatg attggttatt tccgagtcgg caacaaggtc
     2221 gacatataac aagggatcgt gtctatcaga tatatgcaga tatagcagaa aaattaggac
     2281 gagatgatat aggaacacat acattaagaa aaacttttgg gtatcactac tacaagaaaa
     2341 ctagagacat tgctacctta atgtttattt ttaatcatag ctcgcaagca attacaaaaa
     2401 ggtacattgg tataactgaa gatgaaatag gagcatcact tcgtggattt aaactaggtg
     2461 tataatttaa aaatttttat aaacagtaaa gacaaagatt aaaagctatt tttatctaga
     2521 acgattttat ttcataaagt tcgcggaata aaagctgggg ttactggtac ttggtatagg
     2581 ttcgtgttat ataaactaag taattaaata acatttttag gttggaaaag gatgaaatta
     2641 aaaaaagaga atcaatacgg tctcaaaaac gcaaacgctg cggaataaga aaaatactga
     2701 taactcaacg tttagcagac tatctaatag ggatagtctg cttttttcgt ttttgaccat
     2761 acttttgacc ttctgcaaac ttccttgctt ttcctctgta gaactcatga aatctgcaaa
     2821 acgaattgcc gtttcctctg tctgcttttt cgcgatatgg gtgtatatat catccatagt
     2881 ggttttaata tctgtatgtc caagtcgaac atacacttct ttgacagacg caccagattc
     2941 aaagagcaga ctacaatggg tatgacgaaa accatgaagt gtgatatgtt ttaagttatt
     3001 ctctttaatg atgattttca aattgtgatt tacatagtct aaataaaggg gcttattttc
     3061 ttctgatgaa aacaaaagtt gttcaccatc ttttataggt ttgattctca tttttagata
     3121 ttatgcccgc acgtattcat cacatttcta ttgtgaatcg ctttattcga ccgacatttg
     3181 acttttatca taatatactc ggtttaaaat tactaatgaa aacaattaat caagacgatc
     3241 ataccatgta tcacctgttt ttttcagata atcaccaccg tgtgggaact gaactgactt
     3301 tttttgaagt acaagaaggg aacaaccagt tttttggaac caatacgatt gaacgaacga
     3361 tactaaaagt tccttccgaa gcatcattgc atttttggga aatatatttt gaaacacaag
     3421 gcgtttgcca ttatggaatc gaatcatttt ctggacgacc tatcttacgt tttgaagggc
     3481 cagatgcaac acgattagct ttagttccct tacgcgaatt tgaagatatt gatgattatt
     3541 tcccgtatgt cacagaccag atccctacag agcatgccat tttaggaatt gatgccattc
     3601 aactacgcgt acaatattcc gatgcgactg aacgagagtt attgtcggta ggctggtagc
     3661 acaaagaata cgtcagtttc ttttcgggtc atcaacgagt gacaatctta gaaaatcaag
     3721 acactcagtt ctatcaagaa gttcagatta ttcaagatcg tgagaatcca gttgccattg
     3781 aagggattgg tagcgtccat catgttgcgt ttggcgtaga aaataaatct gatttacaac
     3841 gcatcgacaa acaattacaa gagcgcaatt ttataaattc aggaataaaa gatcgtgaat
     3901 tttttgtatc actttattat cgtgacccaa atcaattatt gattgaaatt gctacagggg
     3961 aaggaaacct agatgcaaaa gcgtatgaaa atcaaagtcc acgctttgaa gaaattcctt
     4021 tatttttacc gcagtactta aatttcattc gtgaacaagt ggagcaataa ttagctaatc
     4081 aaatccatga cgaagagtaa gaggttgact cataagtcaa aaaataaaga aaaacgcatc
     4141 aaatccattc taaagaatgt tgatttgatg cggttcctat ttttataaac tatcattttc
     4201 gactaataaa actacttatg agtcagcctc tctttttttc ttgattttta tacctctttc
     4261 taggtaaact aaacatctgc caaaaatacc gtgtctcatt gaaacattct caactatttc
     4321 aaaataaagg ggagtaccaa atgaacgagt atattgaaat tatccacgca agagaaacaa
     4381 cttaaaaatg tcagtctcaa aattcccaaa aataaaatta gtatttttac cagcgtatct
     4441 ggttcaggaa aatcatcgat tgtttttgat actattaccc aagaagctgg tcgtcaactg
     4501 aatgacacct atgatagttt tacccgttta tttttaccta aatacagtcg tccagaggtt
     4561 gatgagattg ataatctttc tacggcgatt gtggtcaatc aaaagaagat tggtggcagt
     4621 gcgcgctcaa cgttagggac gattactaac atcaatgcgt tatttcgtgt gttgttttca
     4681 tgttttgcaa cacctagttt aggatatgca aatgcttatt catttaatga tcctagtgga
     4741 atgtgttctg tctgtcaggg aatcggtaag acgattgcat ttttaatagg attgctttat
     4801 ttttcggcta ttgataatgc tgattttgta ttttctgatc catttaccgc agaagttttg
     4861 tctggaattg aggaacaggc aaagaatcat ggttattttg tattggtgca taatgttaat
     4921 tcagcagaag atgttaaaac tattcagaga aattggcgtt ttgaggggtt cattgttgtt
     4981 ggtgtacgtg taaaagaatt tttggaatta aataacgttt ttgatggtcc tgttgtttat
     5041 attgatacgc atctttcaaa agagacatta agagaacttg aacaacaaat gaatcgttta
     5101 tttgtaaata caaatgatta ccaagctagt acattggcag tggaacattt gattcattat
     5161 ggtcatgaga aaatagcgtt tctttctttt gactacattg taggtgaacc aagtgttatt
     5221 caagaacgac atcaagctta tatcgataca ttaaagaaat atcatttaac ctttaaatcg
     5281 cattggatat ataacaactc tgaatttgag cgaatttatc aagaattagc tgaatttact
     5341 gcaattgttg ttactggaga ttatttagcg gctaaattag taaaattttt aaaaaataag
     5401 aataaacgag tgatggaaca attatcgatt attggatttg atgatattac ttttgcagaa
     5461 ttaatggatc ctgcgttgac aacaatacga ctaaatccag gtaacaaggg ggcgattgct
     5521 tttcaccaac tgattggact ggccaaacaa gaagaaataa aaaatcaagt actactatta
     5581 gagggagaat tgatttctcg agactctgtt cacaaaatca aataaaaaag ctctgtgaaa
     5641 taagtatagt taacaataaa atatccgaac gatagagaga atgaactgtg atctagcagt
     5701 tcctcgtcgc aaattcacta gaattagcat tatttactct aaattgttcg gatatttttt
     5761 cttgttttta attatgatta cagtgcctat ttttaacgaa atttgcttat tttcaaaatg
     5821 tatgattata tgttagtaaa cactttcaaa tatgaaaaga ggagctatta tggtaggaat
     5881 tagggatgtt gcaaaacggg cgggtgtgtc cattgcaaca gtatcgcgcg tgctaaatga
     5941 ggatccgaca ctttcagtaa ccgatgaaac caaacgaaag attgcagaat cggttaattt
     6001 ttatcattat gtcaaaaaaa gtcccgttgc aaaaaattta acaaatattg cattaataac
     6061 gacagtttct gaagtagatg agttggaaga tccctatttt cgaacgattc gcagagggat
     6121 tcaagtcgaa gcagaacaaa gcaaagtcaa tttgaagaaa attattcgtt tgtcagaaac
     6181 accgttggag caagacgatt tgctaaaatg tcaaggtgtg ttgattattg ggcaggtgct
     6241 atcttctgtg attgaagaag tatcagcaat taattcgaat gtcgtagtga ttgatgaccc
     6301 tagtattgaa gctgaagtag atgcagttta tactgattta acaaaagcaa cgaatcagca
     6361 tttagatcga ttgtataaaa aaggacaccg aaacattgcg tttatcggag gaggacgtgt
     6421 tcgtttagat agccaaggaa ataaacacgt gtcagatgat gattatcgaa aaatagccta
     6481 tgagacgtgg atgaaacaaa aaggattaga aaaatatagt caaatttatt tagaaggctg
     6541 gaccacactt gacggaatgc aggcagcaga tttacttttg aaaaaacatg ctaaagataa
     6601 gttgccgact gctatcatgg taggaaatga tcctattgct gtgggggttt atcgttcact
     6661 tcaaaaaaat ggtctacata ttccagaaga tatttcgatt gtgagttttg ataatattga
     6721 agttgctgaa tttttaacac ctgcattaaa tacggtgaat gtgaatacag aagaaattgg
     6781 aaaactagca gttcgcatgg ttaatgaacg aataaaagaa gttagaaatg tttcgatcaa
     6841 agtgattgtt tctaataata ttattgttag agaaagtgaa aaaaatatcg attaaaaaag
     6901 taaacatcca aaaaagggtg tttacttttt tgtttactaa tatttttagt aaaaaatctc
     6961 gacaaaacat taagtaaaca gttatggtgt tgttaacaaa aagaaaacgc attcaaaaca
     7021 ggaggaatta agatgaaaca tttcaaaaaa atagctttaa cagggatagc tgttttaact
     7081 gggacattac tcgcagcatg tggtggggga gatagtaatt ctaaagacgg gcaagtaact
     7141 ttaagctatg cgagttgaga tactgaccaa gctccaggac taagaaaaat gttagatgaa
     7201 tttgaaaaac aaaatcctga tatcaaagta gaaatggaga caacgccttg ggatcaatat
     7261 tgggttaaat tagaagctgc aacaacggga ggtaatatgc cagatgtagt aaccatgcac
     7321 tctagcgaat cctataaata tatgtcacag aatgctttga tgggacttga tgatgttatc
     7381 aaagaaaatg atattgacat gggaaacttt actgaaggaa ttgctgattt ctatacgctt
     7441 gaagacgaac tatacgcaat ccctaaagat gcttctgtag tggggctatg gtacaacaaa
     7501 gaattatttg ataatgcagg tgtcgattat ccagatgaaa catggacatg ggaaacactc
     7561 agacaagctg caattgattt aacagatgaa tcaaaaggca tctatggttt tgcatcacat
     7621 attggaacag agactggcta ctggccattt atttatcaaa atgggggaga agtatttgcc
     7681 gatgacaata caaaatcagg tttgaatacc ccagaagcac aagaagcttt agctttttat
     7741 cgagatattg cacttgttga tcatgcttca ccaacaatta cagaattaca agatcctgat
     7801 aagttaagtc gttttcaatc aggtagatta gcaatgatga ttgaaggaaa ctggtttgca
     7861 ccgggatttt tgaaaaatga ctatctagtt gaaaatgttg cttctgccgt gttacctaag
     7921 ggaaaagtta atgcaacaat tacaaatgga cttggttggg ctgccagtgc gagtacaaaa
     7981 tacccagaag aagtgaaaaa actaatggca tttttagcta gtgaggacgc caataaaatc
     8041 caagcggaaa cgggggcatc aatcccagca ctaaaaggct ttgaagacga gtgggctgct
     8101 tcttcagata caattgattt gagtgctttt ggaaaaatgt tggagtatgg agtccctcgt
     8161 ccatacaata aagatggatt acgagctgaa caagcagaag ttcaaattat taaccgtatt
     8221 ctgtctggtg aagtaactgt tgaagatgga accgctcaaa ttgaaaaaga agtcaatgcg
     8281 atattgagcg gaaattaaat tagttaatta tacaatatac aggcgaaagg attctatttc
     8341 gtctgtatat taatggatga ataccaaaaa gggggatatt atgaaaaatc gaatttttgg
     8401 aaaatatcgt aaagatcaag tgatatgggg ctatattttt atttttccaa cggcattagg
     8461 attaatttta ttaaatatat ggccagcaat tcaaacagca attttaagtt ttcaaaaaac
     8521 ggtagggttc ggtgatactg aatgggtcgg attaagaaat taccaaaaaa tgtttgcaga
     8581 cggtgaagtt tttcagtctt tattaaacac attaatttat gcagtggttt cgatacctag
     8641 tattgttgta ttatccttgt tggtggcagt tttaatgaat agaaaagtta aaggattgtc
     8701 gatttatcgg acaatttatt ttctaccagt cgttgctgca ccatccgcag tcgcaatgat
     8761 ttggcgctgg atgttcaata atgattatgg actgattaat aacatgttgg ctaaaatagg
     8821 tctcgatgga ccagcatggt taacggatcc atcgattgcg atttattcta ttatcattgt
     8881 aggagtttgg agtgctattg gttataacat ggtgttatta ctagccggct tataagaaat
     8941 tcctaaagat tattatgaag cagcggaaat tgatggtgct agtccaatcc agcagttttt
     9001 caacattacg ttaccattag ttacaccaac attatttttt gtagttgtaa caacagttat
     9061 caatgctttc caagtatttg atgttatttt tatgatgatt actcctaata gtaccgccat
     9121 gtttaaatca caatcattag cctatttatt ctataaacat tcatttattt taaatgacaa
     9181 gggatatggt tcggcaatcg ttatgttcct tttagtgatt atattaatta ttactgcaat
     9241 tcaaatgaag ttgcagaaaa agtgggtaaa ctattaagga ggctatcaaa ttatgaaaaa
     9301 taaatttttt tcaaatgcgc caatccatat catgcttatt ttaggtggta ttgcaatggc
     9361 attacctttt atttggatga ttttaacttc agggaaaacg ttggttgaat ctacccaaat
     9421 tccacctaca attttccctg agaaatttca gctaagtaac tatcaacaag tacttgattt
     9481 attaccattc atggcttttt atatcaatac atttttaatg gttgttggac gagtgattgg
     9541 ctcggtttta ttttcagcaa tggctgcata tgcttgtgcg cgtctaagtt ttccagggaa
     9601 aaatatcctt ttcagcttag tacttattca aatgatgatt cccggttcca tttttattac
     9661 tccacagtat ttattagcac aaaaattggg tattttaaat acaattccag cattaatatt
     9721 accaggggta gttagtgcgt ttggtacgtt tttattaaga caatttttct taggtttacc
     9781 tgatgaattg gaggaagcgg cctatttaga aggtgccaat atttggacga tctttttcaa
     9841 aattatgttg ccgcttgcta aatcaggatt ggttgcactt ggaatattca cagcgttgtt
     9901 tgcatttaaa gatttaatgt ggccattgat tgtcaatact tcaattgata agatgccttt
     9961 agctccggga ttagcaaact tacaaggaca atatgccacg aacttcccac aattaatggc
    10021 aggttcgtta ttagcaattt ggccaatagt tgtgatattc atcgttttcc aaagaaaatt
    10081 cattgaagga attgctacaa caggtagtaa attataaaac tattaattag tgattggaga
    10141 gtaagtacat gacaaaaatt acatttattg gtgcaggaag tactattttt gcaaaaaacg
    10201 tattaggcga tgcgatgtta actccaagtc tacaagatgc acatattgca ttgtacgata
    10261 ttgatgaaaa acgactaaaa gaatcaaatt taatgcttaa aaatattaat aaaaattcga
    10321 atgaaaatcg tgctgatatt gtttcttatt atgatcgcaa agaagcgtta agagaggctg
    10381 attttgttat caatgctatt caagtgggtg gttataaacc ttcaacagtg attgattttg
    10441 atattcctaa aaaatatggg ttgcgacaaa ctattggtga caccatgggg attggtggga
    10501 tttttagaac cctacggaca ctaccagtaa tgttagactt tgctaaagaa atggaagaag
    10561 tttgtccaaa tgcttggtta ttgaactata caaatccgat ggcgatgtta accatgggtg
    10621 ttttaaaagc gacgagtatt aagactgttg gtttatgtca tagcgttcaa gtatgcgtac
    10681 cagaattgtt tgagcattta ggaatagccg atcaatatga cttaaatgac tttcaatgga
    10741 aaattgcagg cattaatcat atggcattct tgttggaaat tacaaaaaat ggggaagatt
    10801 tctatccaac aattcgtcag ttagctgcag aaaaaccaaa tccacatcca gattctgtac
    10861 gttttgaatt gttgaaacgt tttggttact atattacaga atcaagtgaa cacaatgcag
    10921 aataccatcc atactttatt aaatcaaaat acccagaatt gattgacgaa ttagggattc
    10981 cgttggatga atacttacgt cgttgtgaac aacaaattaa tgattgggaa agcatgcgag
    11041 aggacattgt aaataatggg gagttaacac atacgagaag ccgtgaatat gcttcatata
    11101 tcatggattc tattacgaca ggaactccgt cagtgattgc aggaaatgtc ttaaataaag
    11161 gcttaatcac aaacttaccg acagatgttt gtgtggaagt accttgttta gtcgataaaa
    11221 atggcgtgca accaacctat gtcggtgaat tgccaacaca attagctgct ttaaatcgga
    11281 cgaatatcaa tgttcaagaa ctgacagtag aagccgcttt aacactagat aaatcaaaaa
    11341 tttatcaagc agcgtattta gatccacatc tatcatctga attgtcttta acagatatca
    11401 aaaatttagt ggatgattta atcgaagcac atggggacta tttacctgaa tataattaaa
    11461 aagaagctga aagagtaagg aattttcaat tccttactct tttcttagtt aaatttagta
    11521 aaaatattga caaaacactt agtaaacagt tacattagtg ttaacaaaaa gaaaacgcat
    11581 tcaaattgta taaggaggag cataaaatga agtggtctaa aaaaattatt ttagcttgta
    11641 tagcactagg agcaacgatc tctttgactg cttgtggaaa aaatcaggaa gatgcaagcg
    11701 gtaaggtaga aatagagttt tttaatcaaa aaaaagaaat gactcaaacg attcaggaga
    11761 ttgctaaaga ttttgaagca aaaaaccctg atattcatgt aaaagttgtg gatgtaccga
    11821 atgctggtga agtaattaaa acacgtatgc ttgcaggcga tgtaccagat gtcattaatc
    11881 tttatcctca aagtatagaa ttgaaagaat gggcgaaagc tggttattta gaggacttaa
    11941 ccgaagaacc gtatttagag aatatcaaaa atggatatgc acaacgtttt gctattgagg
    12001 atcgtgtata tagcatacca ttaaccgcta atgtctatgg tttttactat aacaaaactg
    12061 cttttgagga aatggggatt caagcaccag aaacttgggc agaatttgaa aaaatagtag
    12121 ctgagattaa agatcaaaac aaagtaccgt ttgcgattgc aggagcagaa ggctggactt
    12181 taaatggtta tcaccaacta gctatagcga ctgctgctgg tggtgaagag gaagcgaaca
    12241 atgtttggcg tttctcagaa gttaatggaa ttaatgctga aagcaaagaa atgcaaaaag
    12301 attttaatcg attagatttg ttaagagaac caaaagcttt gcaaaacaac tggcaaggtg
    12361 ccggttataa cgatacagta gttactttta caaaaggtga tgcgttgatt atgccgaatg
    12421 gttcatgggc aatgccgatg attcattcac aaaatccaga ttttgaagta ggaacttttc
    12481 cttttccagc agatgaagca ggtcaaagtc taacgattgg cgcaggagac ctagccttat
    12541 cagtttctgc aacttctcaa cataaagaag aagccaaacg ttttgtagaa tatatgacaa
    12601 cacctgaagc gatgcagaaa tattatgatg tagatggtgc accgtgtgct gttgagggcg
    12661 ttatcgagaa tactcaggat tcaccattaa gtggtttaac tgaacttgct tttactgatc
    12721 gtcatttagt ttggttagcc aaagattgga atagtgaaaa tgatttttat accttaacaa
    12781 caaattattt acacaatggc aatcaaaaag ccatgattaa cgcattaaat gctttcttta
    12841 atccaatgaa agctgacgga aggtaggaaa ataaaatgaa aataaaagaa attttaaatc
    12901 gttactgggc aatcattttt gtagcaattc caattatttt gcagatgatt tttttctact
    12961 ttccaatggt tcaaggtgct ttttacagtt taactaattg gactggtctg acttataatt
    13021 ttgattttgt gggtttgaac aattataaga ttcttttttc tgatccgaag tttatcaaat
    13081 cgattggctt cactttaatt gtgacgcttt gcatgattgc tggacaaatt ggttttggta
    13141 ttttgattgc gcgggcgttg aacgcaaata ttaaagggaa aacattcttt agagcatggt
    13201 tcttttttcc agcggtctta tcgggcgtgg ttgtttcttt agtatttaaa caaattttta
    13261 actatggttt accaaaaatt ggtagtatgt taggcattga atttttacaa accagtcttt
    13321 taggaacaac atggggagcg atttttgcag taatttttgt tttactttgg caaggaattg
    13381 ccatgccagt catcattttc ttagcgggtt ttcaaagtat tccacaagaa attattgaag
    13441 ctgctagaat ggatggggcg aatgataagc aaattttttg gaatattgaa atcacctact
    13501 tattaccaag tatttcaatg gtatttatta tggcattaaa aggtggctta acagcatttg
    13561 atcaaatttt tgctttaact ggtggtggac caagcgatgc cacaacatct ttggggttac
    13621 ttgtttataa ttatgctttt aaaaacaatc agtttggtta tgcgaatgct attgctttag
    13681 tcttatttat cttaattggg attgtttcac tggcacaatt aaaattatcg aaaaaatttg
    13741 aaatctagga ggtttcccaa tgaaaacatc aaaagaaaaa aatatttcta aatacttgct
    13801 tttattatta gggggcttca tgattttagt tcccttagtt tcaacgctat tcggttcatt
    13861 taaaacaaca cccgatatta tgaataattt ctttcgtttt cctaatccag cgactttgga
    13921 aaatttccga agattgttag cggatggaat tggtcattac ttttggaatt cggcaatcat
    13981 cacaatcatt gcagtagttg ctgtgacagc aattattcct atggctgctt attcactagc
    14041 aagaaaaatg actcgtggga aagcatacgg attgatgtat ttatttttaa ttttaggaat
    14101 ttttgttcct ttccaagtaa ttatgattcc gattaccgtt atgatgagta aaattggttt
    14161 agcaaatatg tggggattaa tcttactcta tctcacttat gccgtaccac agacactatt
    14221 tttatatgtt ggttttatta aactaaatgt gcctatcagt ctggatgagg ctgctgaaat
    14281 tgatggggct agtcacttta caacttattt caaaattgtt ttcccaatgt taaaaccaat
    14341 gcatgcgacg actattattc ttaatgctct atggttttgg aatgatttca tgctaccatt
    14401 attaatttta aatcgtgatt ctaatatgtg gacactacca ttgtttcaat ataattatac
    14461 gggacaatat tttaacgact atggtccaag ttttgcttcc tatacagtag gaattgtcac
    14521 gattacacta gtttatcttg ctttccaaag aagtattata gctggaatga gcaatggagc
    14581 agtgaaataa ccaataaatt attctcatta taaaggagtt cataaactta tgattatatt
    14641 tgacaatcac acacaaacat ttcatttaca aaataaaaaa attagctatc tattaggaat
    14701 cgaagaaaat aattatttaa cgcatcttta ttttggaaaa aagattacgc actattctgg
    14761 tgggtatcgc tacccccgaa cagatcgttc cttctcaccc aacaatgcaa atgcgcaaga
    14821 tcgtttatta tctttagaca cattaccatt agaatttcca ggatatggac atggtgattt
    14881 tagagaaccg gcgcataata ttaaattagg aaacggtagt cgtgtaaatg attttcgtta
    14941 tgatagctac gaaattcaaa aaggaaagcc cagcttagcg ggattgccag ccttatatac
    15001 ggaagatgat gatgaagctg aaacgctgat tattcaattg aaagacgcga ttagtggatt
    15061 gactttacag ttgttctaca caatttatca aaatcacagt gtaattattc gttctacgca
    15121 attagtaaat aatagtgatg aaacagtgga gattaaccgc ttagctagtc aatcaattga
    15181 ttttccaaat cgtccactcg agttaattca tttaaatggt acctgggcaa gagaacgtca
    15241 aatgactaga gaaaaaattc atacagggat aaaagtatta gatagtaaac gcggatcaag
    15301 cagccatcaa caaaatccgt ttgttgcttt agtagatgcc aaaacagatg aatttcaagg
    15361 agaggcatat ggatttaatt tagtttatag cggtaatcac gaaacggtga ttcaaaaaga
    15421 tccatttgac caaacacggg tcattatggg aattaattca tttaattttg gttggcaact
    15481 aaaaactggc gactcatttc aaacgccaga agtcgtgatg gtttatagta atgaaggtct
    15541 gaatacgtta tcacagacgt atcatgaatt gtataatcac cgtttaattc gtggaaacta
    15601 tcgtttaaaa gaacgtccca cgttaatcaa caactgggaa gcaacgtatt ttgattttaa
    15661 cgaagaaaaa atcatgaata ttgtcgatga tgcgaagaac ttgggcattg aaatgtttgt
    15721 tttagatgat ggttggtttg gcggtcgtga aaatgacttc acttcattag gtgattgggt
    15781 tgaaacagaa ggcaaacttg cttgtggttt ggaaaattta gccaagcaag tgcatgataa
    15841 cgggatgaaa tttgggattt ggtttgaacc ggaaatgatt tctgaagata gtgaactatt
    15901 tcgagcacat ccagattggg cattgggtat tccaggtcgt gaccgctcaa ttagtcgaaa
    15961 tcaatatgtt cttgattttt caagacaaga tgttcgtgat aatatctatc aacaaatgac
    16021 ggcgattttt gatcgtgtac cgattgatta tgtgaagtgg gatatgaatc gaaatatgac
    16081 agaagtctat tctgtcttgt tggactcaac gatgcaaggt gaagtggctc atcgttatat
    16141 gttagggtta tatgaatttt tagaaaagtt aacaacgaat tatccaacta ttttatttga
    16201 aagttgttcg ggtggaggcg gtcgttttga tgcaggtttc ttgtattaca tgccacaaac
    16261 ttggacgagc gacaataccg atgccgtggc tcgtctgaaa attcaatatg gaaccagctt
    16321 agtgtatcca atttcaagta tgggtgcgca tgtttcagca gtcccaaatc atcaaacagg
    16381 aagagaaacg agtttaacta ttcgtggcaa tgctgcgatg gctggtgttt ttggttatga
    16441 gctagatcta acgcaattaa cttctgaaga aaaagcagaa attaaagaac aagttgcctt
    16501 ttataaaaag catcgccaac ttttgcaata tggaactttt tatcgcttgg aaagtccgtt
    16561 tgaaggcaat gatactgcat ggatgtttgt ctcaaaagac caaacagaag ccatggtctt
    16621 ttatttccga gtattagcag aagctgcgca tccattagtc acattgaaat tagcaggatt
    16681 agcagaagaa gcaacttatc aaatggaaga tgtaacattt gctggcgatg aattgatgaa
    16741 tctcggtttt tatattgact cagaactaca tggtgattat gcgacacaac gcttttattt
    16801 gaaacagata tagtagcttg aatttacaat ataagtgcaa caccaaaaga gactcattcc
    16861 aaacgaagaa aatatgtttt atttacacgc atttactaaa aagcagcctg atttgaattg
    16921 ggaaaatgaa gaagtacgtg aagaaatcta tgcaatgatt aactattggt tagataaagg
    16981 actaggcgga tttagaatcg atgcgatatt aaacatcaaa aaaaaactag agtatggcat
    17041 ctttgaatct gatggagaag atgggttagc cttcattggg ccctggattc ttaatcagtc
    17101 gggcattgaa gtgtggttaa aagaaatgag agagcggaca tttaaaccac ataatagtat
    17161 gacggtagca gaagccgatg ttcctaatga acgtttagat gaatatattg gtgaagatgg
    17221 ctattattcg atggtttttg attttagtta tacggatatt gatgtacctg aaactggtga
    17281 atggtttaaa gatagccaat ggacatggac ggatatgcgt accaatattt ttacgaatca
    17341 attagttacg caagataaag gttggggcgc cttatattta gaaaaccacg atcaaccacg
    17401 ttcaattaat aaatatattc cagaagaatg gatcaatgat tatagtaaaa aaatgttggc
    17461 gacgcttttt atgttactaa gaggtacccc ttttatttat caaggacaag aactaggaat
    17521 gaccaacata gaaatggcat cattagaaga ttttgatgat gtagctacgc attctcagta
    17581 taaacgggca ttagaatatg gattggcacc agagcaagca ttgaaagctg tcaataaacg
    17641 aagtcgggat aattctcgca cacctatgca atggaatacg caaaaaaatg ccagattctc
    17701 aacttctgat aatgtatggt taaaagtaaa tcctaattat ccagaactca atgcagaagc
    17761 tcagttaacc aatcaacagt cggtattcaa ttattatcat caattaatta aattaagaaa
    17821 aaatagtgta tacaaagacg tgctaatcta cggacaattt ctaccagtga aagataaaaa
    17881 tgaacatatt ttgctttatg aacgccaatt agaagataaa atcgtattag ttatcttaaa
    17941 cgttacagca acagaacaag aatattatgt agatgaacag taccaacaag tattaatcaa
    18001 taattatgaa gatgttttat tagatcaaag tcgaaaattg cgtttacgac cgtttgaaag
    18061 tattgtatta gctaattatg gaggacaatt atgaaaatta aaaacgaagc aatgctcatc
    18121 acctatgcag atagtttagg aaaaaacatg ggcgaattaa aacaagtctt agacaaacat
    18181 ttacaaggtg ttattggtgg tgtacatctg ttgccttttt ttccatcgac aggtgatcga
    18241 ggctttgcgc cgagtgatta tacgacagtt gatccagctc ttggaacatg ggaagatgtg
    18301 gaagcattag gtgaagatta ttatttgatg tttgatttta tgattaatca catttctcgt
    18361 gaatcaaaat ttttccaaga tttcaaaaaa aaccatgagc agtcaccata caaagaaatg
    18421 tttatccgta ttcatgaatt tttcccagaa aatcgaccaa cacaagaaga tattgattta
    18481 atctacaagc gtaaagacaa agcgccgttt caaacggtag aatttgcgga tggtacgaca
    18541 gaagaggttt ggaatacctt tggggaagaa caaattgatt tagatgtgac caaagaagtt
    18601 gtgaaagaat ttattcgaga aaccatcaaa gatatggcga accatggctg ttctttaatt
    18661 cgattagatg cgtttgctta tgcgattaaa aaattagata ccaatgattt cttcgtggaa
    18721 ccagatattt gggaactttt agacgaagtg cgtcaagaag cagcgaaata tgacgtcgaa
    18781 ttattaccag aaatccatga gcattattcc attcaaatga aaattgccaa tcatgattat
    18841 tatatctatg acttcgcctt gccaatggtc actctgtatt cactttatag tggaaaatca
    18901 gaacgtttag ccaattggtt gaaaatgagt ccgatgaagc agtttacaac attggatacg
    18961 catgatggaa ttggtgttgt ggacgctcgt gatttattaa cagatgaaga attggattac
    19021 acctctgagg aattgtataa agtcggggcg aatgtgaaga aggtctattc aagtgccaat
    19081 tacaataatt tagatattta tcaaattaac agcacctatt atagtgcatt aggcgataat
    19141 gaccgtagct atctgttagc acgtgcgatt caagtatttg cgccagggat tccacaaatt
    19201 tattacgttg ggttattagc aggaaaaaat gatattgaac tgttggaagc aacgaaagaa
    19261 gggcgtaata ttaatcgtca ttactatgat ttagccgaaa ttgaagaaga agtgcaacgg
    19321 ccagtgattc aacaattatt tgcactttta aaatttagaa atgaatctgc ggcgtttgat
    19381 ttggatggaa cgattgaagt agcaacgcca tcagaatcag aaatagtgat tacacgtaaa
    19441 aataaaaacg gtagtgatag tgcaacatta acggctaact tggatacgaa agaatttatc
    19501 attgagtcta aagatataaa aattttcact agtgttgcat taaaatttta acctattcca
    19561 tagaacaaaa aatcctttat actagcactt ggatgactta tccaagacca ataaaaagga
    19621 ctcatacatg gataagtata aaacaaatac agcatttaat aaatggtttt ctgccataaa
    19681 attaggaaaa ctttctgtca actcccaaaa atgtattttt gattacaatt cgtatcgtaa
    19741 aaaactgtct tttgaagctg tgctcaagct ctttctctac gcagttaatg aagaaaaaga
    19801 aagtttgcgt gatttgagta cagctcttat cgaccgatcg ctccaactag aaaccgatgt
    19861 gtcatcgatt agtcataccc aattgtctcg agcatttaat gcacttgaac ctaaagtgtt
    19921 agaagaaatt tttcaacaac tgttagaaaa agttcaacat caaatcaaac caaccaagcg
    19981 aaatcatctg tatctgattg attccagtac gttttcgctt agtttaaaac gtcataagtg
    20041 ggcgactttc cgtcaaacaa aatcaggtgt caagttacac ttaaactatt gttatatgga
    20101 cgatactacg atgtatccaa ctgattttac cttaacaaat gcccatgaac atgatctcaa
    20161 tcaactacca gtattggtga atcaaccaga ggcaacttac gtgtttgatc gtgggtatta
    20221 ggactttgaa aagatggatc agatgcattg ggatggctat ttcttcgtca ctcggattaa
    20281 aaaaaacacc aaagttcacg tgatagatac tctggaaaca tcgccagaaa cagaaattct
    20341 acgagacgag ctggttcgtt taggatctaa aacctatcta accgccaatt tccgactagt
    20401 cactgttcaa gataagaacg gacgtgtgtt tcaatttatc accaatcgaa tggacgtctc
    20461 ttcaaaagaa atctctgata tgtatcacgc tcgttggcaa atcgaattgt ttttcaagca
    20521 catcaaacaa cacatgacca ttaaaacctt cttttctcaa agtgaaaaag gcgtgcagaa
    20581 tcaattgatt ttaacgatga tttccgctct cctcacgttt ctcatcaaat tggagacgaa
    20641 aacggaaaaa tcagtgttcc aaatcaaacg attctttcgt tatttactgt ttcagccctt
    20701 tgaatgttgt ctagaaaaat taattcccac atgagaaaaa agaaatttgg ctcgttactt
    20761 ttggttttca gttgaataac agataaaaaa ttttctggaa aaagtcatcc tctgcgtcag
    20821 cacagttttt tttgacttat tttcagagga aatagttttc agaatattct aaaatgtttt
    20881 atgcaacact agtgatattt tttagaatta gatttctaat gtgcattttt tgagtattaa
    20941 ggagcaagta tacaaagatt tataaagctg attaaatttg aataaaaata tgcaggaacg
    21001 ctactatttt tagtagtgtt cttttttata tttgaataaa aaatgtggtg gttaaatggt
    21061 ggttataaca aaaaatgaat taatgaaatt aggttatggg aagtatcagt ctgagagaat
    21121 catacgacag gcaaaagcaa ttatggtttc aaaaagctat acctattacg aaaatagaag
    21181 attaggacta gtacctgttg aatctgtaga agaagtttta ggtatgaaga tagaggtaga
    21241 ggaggatttt cagaatcatg gttaatttta aaaaagataa aaatgggaaa tatcatttta
    21301 ctgctagttt gggattcgac aaacgaacag ggaaaagagt acaaaaaaac gtagaggggt
    21361 taaaacagtt aaagaagaaa aaaagcgtat accgaacttg tttctaatta tggaaaagaa
    21421 gcagaaactt ctcaaagtac tatgttgttt gaggagtttt tttataaatt attcctacct
    21481 tattatcaag ggaaagtaga agaaagaaca tataaaaatc ggttatctac aatcaaaaaa
    21541 acttttctta tttctttaaa atggagttaa gagaaatacg tccaattgac attcaaaagt
    21601 ggcaaaataa tttattgagc aaatataaaa attcttatgt aagaaacatg tatgggattt
    21661 ttcagatgtc attagatcga gcagttgttt tgggactact tgaaaaaaat cctgcaaaag
    21721 ttattggaaa tgtgaagaaa aggaaaacta cagttgattt ttggacaaaa gaagaattta
    21781 caaaagttat ttcaactttt tatattgatg atttgtacca acattttggt tttatttgta
    21841 tgtggttact ttttatgacc gttatgcgtt tgggagaagc cactgcatta acttggaaag
    21901 atattgattt tgaaaataaa aacttgcgtg tgaataaaac actctattat aagagagcca
    21961 aaacctatga gtttgtagaa cccgaaacgg cttctagtat tcgtacaatc gaactagacg
    22021 acaatacaat taccttttta aaagcttgga aggagcgtca agtttcaata ggaggcatgt
    22081 cgtttgtact ttcttataat ggaacaccta ctcagaaata tacaatccgt catattatga
    22141 gaagacatgc aacgatcgtt ggtgttcata taatccgcat acacggatta aggcatagtc
    22201 atgcttcttt gcttatatca caataaaaag atgcacttcg aattaaagaa aggttaaaac
    22261 atgctgatgt acaaacgact ttaggaactt atggtcattt gtatctaaat tcattgagag
    22321 agataacaaa tgatcccaca gattatatcc aaactaattt ttctaaaatc aaaccgacac
    22381 ctatttacga taataaaaaa taaatgataa acaaaagtag gatcacacca aaagaaacac
    22441 actagaaaaa ctataataca ggcttttcta gcgtgttgtt tcttatttcc gcctatttat
    22501 acattatact cataaagcat ttaatgcatc gatcgtagcc gcattgtcta tatgggtata
    22561 taaagttgtt gtattggtac ttgaatgtcc caattgttgg gcagttaata ccaatgaatt
    22621 tgtctgttgg tagagtctag tagctaatgt atgacgcagt ttgtgagggc tgacttgaat
    22681 tctaaagcct tttgaatatt ttgctaccat ccgttcgatt gcaataccgg atatacgctt
    22741 ggcttctcct tggtaaattg ctagaaataa agctttttcg ttttctgtta ctttatatcg
    22801 ttctttccga atagctagat aattagctaa atattccatt gcgaatgctg ctatattgac
    22861 agcatctctt ttatttcctt ttctagttac taccacatta ttcttatcta aagataggtc
    22921 atctaaatta atatttataa cctcggagag tctcaatcca gatcctaaaa tcagtgcatt
    22981 gatcgccagg tctctttcta catctcgttg ataatagatc aaagctcgtt tactcaaagt
    23041 atttttatat tcattggcaa tataatctag ataattttgt gtatccttat ctagaaataa
    23101 ttttttctca atggcattgg cacgtgcagc taacgtagtt ttatctgtga tggttgctac
    23161 ttgatccata ggattattgt ctaagtatgg ttttcctaga ggattattgg aacttttcga
    23221 aagatattta aatagaacct ttaatgccgt gatatttcta ttgatagtag aagtagatag
    23281 acctaagtta ttattatctt tgagcttagt ttttgttgtc tcttttttta cattttcttt
    23341 tgtgcgacgt ttcaaatgag atttgtacaa gtttaggtca taggcagtta actgtcttat
    23401 cgaggtgata gaagtatcag gaacgataga ctcactcaga agccattcat aaaagagctt
    23461 ataatttttt agatattcta aagtggttcg aggtgattga tctttatcct cacgatcttg
    23521 aacaaaatct tttatataat cgggcatatg aagtatgatt gtatctattt tttgatatat
    23581 ttctgtattt ttcatgtcat tcatcctttc aataagtagg ggagaagagt atctctataa
    23641 ttggactata ccaattatag aatgttttgt caattggtat gctctaaaat ttattaattt
    23701 gtttcaaaaa aagcatataa acaggtatac gtttaataat ttaaaataaa tggtatacat
    23761 aaatagggaa ggaaatagaa gggagcaatc taaatttttt gaattttctt aattaattat
    23821 atagaaagaa caatctttct atagagttag acatgagagg gactatgctt gatttaaaat
    23881 caacaagcgt atatactgta tatacaaaga aaaaggaggg agaagactat ggcaattaaa
    23941 gaaagaaaat ttagggaagt ggggaactca gtagtattaa ctatttcaaa ggagtttctt
    24001 aaagaaaatg ggttaaatcc cggagatacg gtttttttag atgaagaaaa acttcgagcg
    24061 gcaattacaa aaaaacaagc cttatctgat gaaattgatt tagcaatatc tcaagcatta
    24121 actgaatatg atactacttt tagagagctt gtcgataaat gagtattcat tacctagaag
    24181 ccaaagatat tataaaaatg aatgtaatcc aaataaaaaa atactctcct aatgaacccc
    24241 ttggtgtaaa ggatgcgaat gctttagaaa tgtgtgtagg gcaacttcga gcctcagctt
    24301 ttggtgaaga ggtttatcct tcgatttatg aaaaagcagc tatcctactt atacaattga
    24361 taaagaaaca tccttttcac aatgcaaata aacgtactgc tttcttagct acttttgtct
    24421 ttttgaaaat caatggacag cttttagcta ttgatcaaaa agatgctgta aatttagcgg
    24481 tatatattgc tacgtatgat aaggatttcg atcaactaaa atatgaagtg atcaacacta
    24541 taaagacaca tacgcagccc atttaaggag ttactgtatg tgtctttata gggttattag
    24601 ctgctatagc gaaataattg aagtaaatta caaatagcta taattcaaat ccaactatat
    24661 agaaaacgta aattttctat atagttggat taagataacg actatttatt ttatcatatt
    24721 tttactatac cctatacttt tacttggtat atttatgaat aatcgcaaat ttacccctat
    24781 actactagtt tgtaagttag attaataaaa atactaaagt tatttgttct tttattaaat
    24841 acacctacaa cttataaatt gatggtattt agataacacg aaagaataaa aataacagct
    24901 gatagtaaag tttgttgacg attgctactc aatcgaaagt gagttagtgc gtatgaactt
    24961 gcttctgaaa acttatgaaa tgaaagacct tttacatctt gcagaaatcg ttactgggat
    25021 taaactaagc aataaaaact aatgttcccc tcattctttg aacttctact atttgattta
    25081 tttattctga taaatgacga tttcaccttc aggatcttca aatcccgtat agaatttatt
    25141 taagatctct tcagtatcag taataggata ggcgataggc cacataccct ttgatggtta
    25201 gaataaactg ctcaaaatct gtatcgtatg ttggctttag catattaaca aaggctatct
    25261 ccaaattata aaccataagc gttgaatcgt tcttaacaat ttcaattctt cctaacccaa
    25321 agtcagaaga tataattacc ggacttgcct tacccttcat gtgggttgtt gagattccat
    25381 atttaaaggg aattgtcggt actatcggcg gtctgaaaaa catcccaaaa ataaaaaaat
    25441 ctttcccctg ctagtctcct aagtcccttt gtagcggata tggcaccata atctcatttt
    25501 atcgaagtga gtcatggttt atctgttggc caaccaaaat tgatacaatc taacgatgca
    25561 ccctctacgg cattctaggg gagtgcactt gcattcaccc aataaaaacc ggcagttagc
    25621 aaagaactta tgtagtcgta tgttcttact gttaacatac aagaggtctc ctttccacaa
    25681 ttgcttagca gtaaacggga atttacaaag ctaatctctt ccattttctt atctttgttc
    25741 taaaagtccc aaatggagca actgtattta catgaataaa tttatatact tcccatacag
    25801 ccgttttggt tgcctcgtct gcccattttc ttttgtgtgc agtgaataga tcttcatcac
    25861 tcattgaatc aatcatcatg ttaatatcat caatattacg attgagaatt tcctctaatt
    25921 ctttcaatgg tagatgtgca taggtatttg taaaccactt atataactct cctaactgat
    25981 tccatttaaa ttgatcagaa ggcgttttaa cagataatcc atttctttca tcattttccc
    26041 attttagaat taaattcgtc caaccaactt gatatgctaa attttcagca ggtgttctat
    26101 ccacttcctc aactctttta tccttcaatt cttcaggaat agtttcaaat tctaaaatat
    26161 atttagtata agtcctattt atttcttttt ttaattcttc tttattttta tatgttctca
    26221 ttatattacc tccataaatt cctaattgtc tcctttaatt catctataat ttcatccacg
    26281 atattttaat ccgttctaca atgcatatct gatatgcatt gactgtctaa ctcatatgtg
    26341 aatgatttat aaaatatccc aacgtctcat ctaaagaaat gttgatcgtc agcaaggagc
    26401 taaaaaatac gaatatattt cttttgtagt aaaatcggac tggtttgcat atcattttct
    26461 tcgtaaatat caaaatcatg ttaactacta tcaattacta ataaaaacta ccactttaag
    26521 cgttaagaac tgttaagttt agttaagtga ggatagttca caaaatccat tgttaaactt
    26581 cacgaaagtg gtaaaactca aaatgcttta tataggatta cagcatcgct ctttcttcga
    26641 ttgcgcgttg ggtaaaaaat attatgaagt aaaaattgac gatgacacgt tccttacggc
    26701 tcatcagatg aaacagctac aaaaacgaaa tgatcaactc gaagaaaatt taattctaaa
    26761 aaagcgattg ccatattcac gcctcgctcg acgaactatt aaaggtcaag atttacctga
    26821 agatcatcct ttttaggaaa ttgaagcttt ttatgttgca caatgcattt ataatactac
    26881 aatgatgttt tcaccagata ttcttctagg gtgcggtgta atgaaacaga tacacttgca
    26941 aaagaaaatt caccgcgaat ttactaaaat tatgaataat tatgttaatc ttccgaaatt
    27001 tttttccaaa attaagggat caagctggtg ttatagattg tttaaggttg gcaaaaaaat
    27061 aacttctttt taaacaatta tagaaaaccg atactctgtt aagttggttg acacggtgtc
    27121 gatttttatg actttcttat tctgaaactt cactaacgga cagtacctaa aaagtctata
    27181 gtatggacgt ttacgagtac tattttatca aaaatgctgc tcatttcaac ttatatgttt
    27241 tagtgaacta cccccactta gc
//
LOCUS       DAZKLM010000031        26723 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3764
            SAMEA115706913-rid22582563.denovo.031, whole genome shotgun
            sequence.
ACCESSION   DAZKLM010000031 DAZKLM010000000
VERSION     DAZKLM010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706913
            Sequence Read Archive: ERR13196647
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26723)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26723)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287823.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287823.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,004
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,849
            CDSs (with protein)               :: 2,849
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 41 of 96
            Pseudo Genes (incomplete)         :: 52 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 15 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26723
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706913-rid22582563.denovo.031"
                     /isolate="VRE3764"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(26..961)
                     /gene="murB"
                     /locus_tag="ACG842_001201"
     CDS             complement(26..961)
                     /gene="murB"
                     /locus_tag="ACG842_001201"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HFQ0247755.1"
                     /translation="MNKEEIVESLATITILFDEPLMNYTFTKTGGPADVLAFPKKQEE
                     VKQIIDYCRIHDIPWMVLGNASNLIVQDGGIRGVVIMLTEMKQIHVKGTMVIAEAGAS
                     LIDTTYAALAESLTGFEFACGIPGSVGGAVYMNAGAYGGEIKDVFAEVDLLLEDGTLK
                     TLTKDEMVFSYRHSKVQELRAIVLEARFSLQTGDYEAIKARMDELTELRQSKQPLEYP
                     SCGSVFKRPVGHYTGQLIQQAGLQGLKWGGAQVSEKHAGFIVNIDHATATDYIELIAH
                     IQEVIKEKFDVSLETEVRIIGEKQAISETSRSLAN"
     gene            complement(1034..1954)
                     /locus_tag="ACG842_001202"
     CDS             complement(1034..1954)
                     /locus_tag="ACG842_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFQ0247756.1"
                     /translation="MKIGVIGVGNIAEKAYLPTYAKKQGTLDFYFATRNKETKERIQK
                     EYGFQHLYETIDELLEEKIEACMIHAATKVHYELAKKCLENGIHVYIDKPLSVHLNEV
                     RELQELADKNQIILMIGFNRRFAPMVEELKRIPDKRLIHLQKNRIAAKETTEFVLYDL
                     FLHLIDTAVYLLDEPILRTKYQIRETKEFLEYAILQLETQNQTAILTMDLASGANIEK
                     YQVTSKNGTYEVNDLTQLVIQDADGKKVKEFGDWTNTLVKRGFEPMVEAFFSQIRLGK
                     SETELLKQKEVVRSHELCEEVLKDHYRHQL"
     gene            2067..2819
                     /locus_tag="ACG842_001203"
     CDS             2067..2819
                     /locus_tag="ACG842_001203"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HFQ0247757.1"
                     /translation="MKFISWNVNGLRAIVNKNFLEVFHELDADFFCLQETKLQAGQID
                     LDLPGYYQYWNYAERKGYSGTAIFAKKPALNATYGMGIDIHDTEGRLITLEYSDFFLV
                     TCYTPNSQSELKRLDYRLEWEEAFYNYLENLKKQKPVIVCGDLNVAHQKIDLKNWKTN
                     QKNAGFTPEERAALSRLLDNGFIDTFRYFYPTQEGVYSWWNYRFNSRKNNAGWRIDYF
                     LTSKDLEPGLADAKIHTDIMGSDHCPVELDLK"
     gene            3683..4216
                     /locus_tag="ACG842_001204"
     CDS             3683..4216
                     /locus_tag="ACG842_001204"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HFQ0247758.1"
                     /translation="MNFVAMDFETANHQPYSACSLALVMVKNSQIVDEFYTLIQPETP
                     FFWRNVQIHGIHQEDVRNAPKFPEVWQTIQPYFQKNRLVVAHNAAFDTKVLAGCLDYY
                     HLEQPNYLSLCTVKSSRRLFPEFPNHRLNTVCENLNIQLDHHHDALEDSRACAEILLY
                     QEKKFGTDPLKKLVTIK"
     gene            complement(4706..5230)
                     /locus_tag="ACG842_001205"
     CDS             complement(4706..5230)
                     /locus_tag="ACG842_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase family protein"
                     /protein_id="HFQ0247759.1"
                     /translation="MSEEVGIILREAVPGDAKDILLMMGQVNKETEFLVLDEAELLLP
                     PETLEEELDYIYESNNNLLLLAIYEGTIIGTASVKADSQFRLSHVGEVGISILQEYWG
                     MGLGTLMLEEIISWAKEMGVLFRLELDVQVRNERAVHLYRKMGFQIEAVMPRGARTDL
                     GEFLDVYKMSYLIE"
     gene            complement(5223..5696)
                     /gene="tsaE"
                     /locus_tag="ACG842_001206"
     CDS             complement(5223..5696)
                     /gene="tsaE"
                     /locus_tag="ACG842_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HFQ0247760.1"
                     /translation="MIELSGLTMTEQFAEAIGHSAMPGDNLILTGDLGAGKTTLTKGI
                     AQGLGITQMIKSPTYTIIREYSQGRIPLYHMDIYRVAASGADLGLDEYFEGDGLSVVE
                     WGNLLEEALPEDYLELILEKSDTDLEKRYVKLISYGEQSETFKQRILEKWAENHE"
     gene            complement(5819..6799)
                     /gene="pta"
                     /locus_tag="ACG842_001207"
     CDS             complement(5819..6799)
                     /gene="pta"
                     /locus_tag="ACG842_001207"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008959 - phosphate acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HFQ0247761.1"
                     /translation="MELFDSLRFKIVRRGIKIVFPEATDARILGAAARLKAEELVEPI
                     LVGNEEEIRNAAHARGIKTSSFTIISPDNYDKWDEMVDTFVERRNGKASKEQAEKILK
                     DVNYFGTMLTYMGIADGMVSGAIHSTGDTVRPALQIIKTKPGISRTSGAMIMVRGRDQ
                     EKYVFSDCAINVNPTAQELAEIAVDSAKTAELFDIEPKVAMMSFSTKGSAKSPEVDKV
                     VEATKIAKKLAPQYMIDGELQFDASYVPSVAQLKAPDSEVAGQATVFVFPELQSGNIG
                     YKIAQRFGNFEAIGPILQGLNKPVSDLSRGANEEDVYKLSIITAAQTLID"
     gene            complement(6952..7632)
                     /locus_tag="ACG842_001208"
     CDS             complement(6952..7632)
                     /locus_tag="ACG842_001208"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HFQ0247762.1"
                     /translation="MKEIIHNSWQEVLSSEFSKDYYLHLREFLKKEYASQKIHPDMYH
                     IYEALELTPYEEVKVVILGQDPYHGENQAHGLSFSVQPGVKIPPSLRNIYKELYDDLG
                     IAPVQHGNLVSWAKQGVLLLNTVLTVREGQAYSHRGKGWERLTDTIIEKLNEREKPIV
                     FILWGKPAQEKIKMIDKSRHIIITSPHPSPLSASRGFFGSKPFSKTNDALLALGEEPI
                     DWQLPETV"
     gene            7758..8627
                     /locus_tag="ACG842_001209"
     CDS             7758..8627
                     /locus_tag="ACG842_001209"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFQ0247763.1"
                     /translation="MIKLIASDMDGTLLDAHMSVSPENAEAIRFANDAGVEFMVATGR
                     NYQEARAALDEVGIDCAMITLNGAQVFDKEGNSLFTVSIPNNQAISVLDILDANGIYY
                     EVATNDGLYSENQAKRIESFASMVATHLPHLTYKMAIAMASAKLELLHITYVDSIRAI
                     LEEKNLEVLKIICFHTEGPAVLGPVGKQISLLGDLAVTSSGQNNIEVNHKNAQKGIAV
                     AHVAHERGISLEEVMTIGDNFNDVSMLQVAGVSFAMGNAEIEVKDYAKYTTDTNLESG
                     VGKAILRAIAEDL"
     gene            8653..9168
                     /locus_tag="ACG842_001210"
     CDS             8653..9168
                     /locus_tag="ACG842_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LURP-one-related/scramblase family protein"
                     /protein_id="HFQ0247764.1"
                     /translation="MSEFFIQEKQLGRATRTIVKNEKGHSLFLMVGRWGTKGDALSLY
                     AMNGDLVAHIKQISLTFGTRFELYKEFEKVGTMQKIFNWPGDFYYIRRLHWTVQGDIY
                     NHQYQIHHFNQQIMKMDKATLFSGDYYVLDVSNDEDAPVCICIAAVLDYWLYRKDKSR
                     DKPVSYRLNLS"
     gene            complement(9215..9778)
                     /locus_tag="ACG842_001211"
     CDS             complement(9215..9778)
                     /locus_tag="ACG842_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0247765.1"
                     /translation="MIRFARKEDAPEIAPLILVILKDMELPFLKQTGEQKTLEILEEA
                     ITDPNYRYSYSRGIVEEIDGKVAGVAYGYTDEEESIIDQPLVPILEKYQLDASTRLFI
                     DKETFPNEWYLDSISVSEDFRGQGIGSRLLEALPKLAKKANRSVIGLSVDEKNPKAKK
                     LYERHDFKVVGQRMISGHLYDHMQKNI"
     gene            complement(9833..10012)
                     /locus_tag="ACG842_001212"
     CDS             complement(9833..10012)
                     /locus_tag="ACG842_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0247766.1"
                     /translation="MEKMKCPNCGKKFAYEEVNNVVEHNDKEMPVVCPYCRTEAARIV
                     THGYFITEKIEDFLK"
     gene            complement(10089..10598)
                     /locus_tag="ACG842_001213"
     CDS             complement(10089..10598)
                     /locus_tag="ACG842_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0247767.1"
                     /translation="MKKMMFGVAALIVIGITGMTGLLNAAFHMDDISDQEYRISLKKA
                     AAIVKNETKEHNISAIHFAVEKDLENPTNATYEYQFFCEGQVIAVNPSTGKTTVRNDT
                     EKAAEHLFDIEKTEEFKQPQAAMKEAVSYYGKKAKVKEWTLLVKDERPYYKVRLIDGE
                     QDRQVWISA"
     gene            complement(10694..11074)
                     /locus_tag="ACG842_001214"
     CDS             complement(10694..11074)
                     /locus_tag="ACG842_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFQ0247768.1"
                     /translation="MNLTTIHHIAIIVSDYQKSRDFYVNKLGFEVIRENYRKERDDYK
                     LDLKLGSAELEIFGIANAPQRPNYPEACGLRHLAFKVDDIEETIAELQSLGIESEPIR
                     EDTFTGKKMTFFFDPDGLPLELHE"
     gene            11404..12153
                     /gene="pnuC"
                     /locus_tag="ACG842_001215"
     CDS             11404..12153
                     /gene="pnuC"
                     /locus_tag="ACG842_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015663 - nicotinamide mononucleotide
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015890 - nicotinamide mononucleotide
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HFQ0247769.1"
                     /translation="MKSYFQFLAHQLKGWPQQNYYLFFFSLGCQVMTLVNSPITWLSV
                     ITFIGTTLGVLCILSINAAKSVNGILGILSAICFIIVGFSAKNYLSIGEQIAYMITLD
                     IPVLLSKEWNHNMASKIRKFTGKSWFIAIISTFLVYLVSGFLIGRYTDDPRPWVDAIA
                     FSISLTAGIISFLRYNNQYYWWLASGLAQMVLWYISFTHGSASLAMFVNSSVYLLNDV
                     LAFTVSPWYNQKERERMQKMEEEYYNAQQHA"
     gene            complement(12474..13691)
                     /locus_tag="ACG842_001216"
     CDS             complement(12474..13691)
                     /locus_tag="ACG842_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HFQ0247770.1"
                     /translation="MPYILALDVSMGHSYSVLYKDDTCLFEDEIEHTQRGFHALLEEI
                     QHLPERPLIVFEATGIYSRPVEKFFQDHHFSYCLLNPLEAKKQMEESTLRSWKTDKSD
                     AHRLAQTHLKNQRQPKEVQSNFYLEMRDLARFYQEIEKKITRLRMDLHNCLQLTFPEL
                     EQFFSNRLTPYALTLIRLFPHPDFVLASTRTKIKNKLINETRKKISANRAEQKADQII
                     HYAQCAYPAVEKDSIHCQKTIYYAELLQDLLEQKKALATQMIKKAEGSPCFLLYQTFP
                     GIGALTAALLLGELGDITRFKTHKQLNAFVGIDIRRYHSGKYTGQDRINKRGNPKARK
                     IIFFTIRNMIRQQRAAPNHIVDYYYKLKKQPIPKKDKVATVACMNKLLKCMHAMVRAH
                     TEYDYAYAVSVDH"
     gene            complement(13949..15226)
                     /locus_tag="ACG842_001217"
     CDS             complement(13949..15226)
                     /locus_tag="ACG842_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0247771.1"
                     /translation="MEEWQSVFEEWFPKEISKSYPIKISKQYTSSQRWEIYAKLTKKQ
                     RELVDKHRRYLISSRFMEEHYLAATDWVFSDFKINPFFRTKRSQQKLYCECGRELKVQ
                     YIVKSPKTGKILKLGINHFADHLHVSPTVAASIHQGMTKVDLALDELLWLKQKNIDFP
                     EGLWQKYCFVLYQNRRMKQPYLPDIKLAQRLAEFRQVEMPIYIADYQALENEIKKISE
                     HINGQSKKRQIKKELFDDFAEELVKDVEEFLINYRAFLRKDWQSIVYEEVPVHPNAYF
                     ETFISVLRKTKRQRTPEVTAQMEYFAKNQRFIQPKIYLFIWKQYCRYGFTEGFFDSIP
                     RIVRNGFLKVLRKEREAIQSADKKDRTVSKEKWQLVVKDIQSGNVQETIDKWKGKHYR
                     FTEAQKQALEYYQKLEESLRFNDEARKYLKELL"
     gene            complement(15305..15949)
                     /gene="pcp"
                     /locus_tag="ACG842_001218"
     CDS             complement(15305..15949)
                     /gene="pcp"
                     /locus_tag="ACG842_001218"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016920 - pyroglutamyl-peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HFQ0247772.1"
                     /translation="MKVLVTGFDPFGGDKVNPAYEAVKKMPDEISGAEIIKVEVPTVF
                     EKSSQVVKEAIQQHQPDVVICVGQAGGRSAVSFERVAINLAEARIPDNEGNQPFDTAL
                     EENGPAAYFTSLPIKAMTKNVQDHGLPAYISYTAGTFVCNDIMYRLLHLIETQFPAIR
                     GGFIHVPFSPDQVIDRPVGTPSMSLEDIASSLTYAVEAAVENKEDISGNAGTTH"
     gene            complement(16148..17092)
                     /locus_tag="ACG842_001219"
     CDS             complement(16148..17092)
                     /locus_tag="ACG842_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HFQ0247773.1"
                     /translation="MDFFTNPDILLGDKLLEILYIVMGLVLIYTGVRNLTDKTNPHRY
                     GSAFFWTVLGVVIAGGRWLPAIVNGGLIFAMTIPAIAKKVSKGESRLPSKEYMEKMSD
                     KLGMKIFIPALSIGVFAILFALFTNLGALVGVGVGVFAAMLILMFFSRDNRPSTFLDD
                     AADMLGTVGPLSMLPMLLASLGAVFTSAGVGTVISNAVGTIVPEGNVNIGIIIYALGM
                     VIFTVIMGNAFAAITVMTVGIGAPFVFSHGANPALIGMVALTCGFCGTLLTPMAANFN
                     IVPVAMLEMKDKYGVIKNQLFIALFMLVFQIAYMILFK"
     gene            complement(17094..17780)
                     /locus_tag="ACG842_001220"
     CDS             complement(17094..17780)
                     /locus_tag="ACG842_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HFQ0247774.1"
                     /translation="MENYLSLLGVLIVILGFAFKLDSILIVMVALVVTALTGGLGIQG
                     MLELLGTSFVNNRGMAIFIIIMLATGTLERNGLKESAASLIKRFKKVSAGMIVDIYGI
                     FRMIFAAFNVSFGGVAGFVRPIILPMALGTIESKGLEVHPKHEEELKGMASAMENVCW
                     FFGQVLFIGGAGALLVQSTLKDLGYDVTLGQLALVEVPVAITALVVASVYFYLKEKKL
                     AKKYYPGKGQ"
     gene            18254..18574
                     /locus_tag="ACG842_001221"
     CDS             18254..18574
                     /locus_tag="ACG842_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HFQ0247775.1"
                     /translation="MAWMELIIAGILEVFWSTMMKWSDGFSKINYSFYTVIGMIASFY
                     FLSKAIKSLPMSLSYPIWTGIGAVGSVIIGVVLFHDKLNLSTWFFVGMLLISIIGIKI
                     TSGH"
     gene            18728..19633
                     /locus_tag="ACG842_001222"
     CDS             18728..19633
                     /locus_tag="ACG842_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0247776.1"
                     /translation="MKSLSVQELSKNFGHKQALSDVSFSLSPGKIVGLVGPNGAGKTT
                     IMKAILGLIHYSDGTICIGENEISPSSHKGLEQVGALIEYPGIYPFLTGYDHLKLFSE
                     TNDVSAIDTIVNQLKMEKYIHKKAKSYSLGMKQKLGIALALLNHPSIVILDEPMNGLD
                     PQATRDVRKIITDLATQGTSFLISSHLLSELEKMVDELILINQGKIIKQCTMAELNQT
                     AQDFIVISTTNDLAARTILTEAGYTLEDPDKIKLLKENEELLGTVIQLLSAQNIHVTD
                     VQHLTNDLESSLLEILDSVKGEEEK"
     gene            19630..20403
                     /locus_tag="ACG842_001223"
     CDS             19630..20403
                     /locus_tag="ACG842_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFQ0247777.1"
                     /translation="MTLLVKQELFKLIKKKSTAVLSVLLVVLLIGTALLAKKYTTIID
                     PVEMTAQLFSATSWIVFIMIAAASTIISMEAQYGTLKNLLYRKYSRGEILVSKWITLV
                     IYSVYLYLLAIIVTVLMKLILFPSISFTEKVSTGQTLIQSLFTYTLGSYIGLWLILSL
                     VLMIACFINSSGASISAGIVFYFASSIISGILIALIQKWEWIKWNPISMLNLQNQIGN
                     EEIMKQLTHLSTNQMLFGNLAYIVLFLALGYLVFKRKNV"
     gene            complement(20584..22008)
                     /locus_tag="ACG842_001224"
     CDS             complement(20584..22008)
                     /locus_tag="ACG842_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="basic amino acid/polyamine antiporter"
                     /protein_id="HFQ0247778.1"
                     /translation="MDTEQQKKGIGLIPLAALVIGSAIGGGVFGIMTDISGSAGGPAI
                     FSWVLVGTAMLMLSLSINNINKKRPDLKGGIFSYAEEGFGRFAGFISGWGYWLTCWLG
                     NVAFATLLMSALGYFFPVFEGGQNLASIVMSTVFLWGYAWLVNRGVENASFVNAIVTF
                     CKLVPLFLFIVVAISTFSIQQFIDNFSSDIILNANGQPIPFSSQVLSCIMVMLWVFVG
                     IEGASVVGSRAKKQSDVGKATILGIFGLIIIYVLVSMLPYGTMTMNQLQTIKAQPAMG
                     YVFEMMVGKWGAVVINGGLIISLVGVWLSWTILPIETMGNMAEDGLLPKAWGKLNKNG
                     APTYAIVLTTLCTNVFLLSLLFTDSAYNFAYTLGTAAIFFTWLFLGLYQMKLSYQRKE
                     WVQFLVGLLASAFQVWAMLVVAFNEVMLVLVLFLPGIYFYLKARKEQNEPVQHIHLDK
                     ILLIGVTMVGVIAMVLFATGMLHV"
     gene            22219..22665
                     /gene="nrdI"
                     /locus_tag="ACG842_001225"
     CDS             22219..22665
                     /gene="nrdI"
                     /locus_tag="ACG842_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HFQ0247779.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEHEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQTFGFPFLADYELRGTPSDVTRIYQILAENNK"
     gene            complement(22730..24712)
                     /locus_tag="ACG842_001226"
     CDS             complement(22730..24712)
                     /locus_tag="ACG842_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose PTS transporter subunit IIA"
                     /protein_id="HFQ0247780.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSTATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKVVPQTSA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVVSSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAAGK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(24726..25622)
                     /gene="murQ"
                     /locus_tag="ACG842_001227"
     CDS             complement(24726..25622)
                     /gene="murQ"
                     /locus_tag="ACG842_001227"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA];
                     GO_process: GO:0046348 - amino sugar catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HFQ0247781.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            complement(25625..26686)
                     /locus_tag="ACG842_001228"
     CDS             complement(25625..26686)
                     /locus_tag="ACG842_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MupG family TIM beta-alpha barrel fold protein"
                     /protein_id="HFQ0247782.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
BASE COUNT         7723 a         5550 c         4801 g         8649 t
ORIGIN      
        1 tacttatttt aagttccatt acttattaat tggctaaact tctgcttgtt tctgatattg
       61 cttgtttttc tccaatgatc cgcacttctg tttctaaaga cacatcaaat ttttctttga
      121 ttacttcttg gatgtgcgca atcaattcaa tataatctgt cgctgtcgca tgatcgatat
      181 tgacgatgaa tccagcatgt ttttcagaga cttgtgcacc gccccatttc aggccttgca
      241 atcctgcttg ttggatcagc tgacctgtat agtgtcctac tgggcgctta aagacacttc
      301 cgcatgaagg gtattctaat ggctgttttg attgacgcaa ttcggtcaac tcatccattc
      361 ttgctttgat tgcttcataa tcccctgttt gcaaactgaa tcgagcttcc aatacgatag
      421 cacgtaattc ttggacttta ctgtgacgat aagaaaatac catttcgtct tttgtcaaag
      481 ttttcaatgt tccatcttca agcaaaagat ccacttcagc aaaaacatct ttgatctctc
      541 cgccatacgc acctgcattc atataaaccg ctccaccgac acttcccggg atgccgcaag
      601 caaattcaaa tccagtcaat gattctgcca aagctgcata agtcgtatca atcagtgagg
      661 cacctgcttc cgcaatgacc atcgttcctt tgacatgaat ctgtttcatt tcagtcagca
      721 tgatgactac accgcgaatc ccgccatctt gaacgatcag attgcttgca tttcctaata
      781 ccatccaagg tatatcatgg attcggcaat aatcgatgat ttgctttact tcttcttgtt
      841 ttttcgggaa tgcaaggaca tcagctggtc cacctgtttt cgtaaacgta tagttcatca
      901 atggttcatc aaacaatata gtaatcgtgg ctaagctttc aactatttct tctttattca
      961 ctgaggattt tcctttcttt tctcaaacgt cctcattatt ctatcacgct taatacaatc
     1021 ttgccagtct tgttcaaagt tgatgacggt agtgatcttt aagcacttcc tcacataatt
     1081 catgacttct tacaacttct ttttgtttaa gcaattctgt ttccgattta cctaaacgga
     1141 tctgagaaaa gaaggcctct accattggct caaacccgcg tttgactagc gtgtttgtcc
     1201 agtcgccaaa ttctttcact ttttttccat cagcatcttg aatgacaagc tgggtcagat
     1261 cgtttacttc ataagtacca tttttacttg ttacttgata tttttctata ttagcgccac
     1321 ttgctaaatc catcgttaaa atagccgttt gattttgtgt ttctaattgt aaaatggcat
     1381 attccaaaaa ttcctttgtt tctcttatct ggtattttgt tctcaaaatg ggttcatcta
     1441 ataaatacac agctgtatca ataagatgta aaaacaaatc ataaaggacg aactctgtcg
     1501 tttcttttgc cgctattcga tttttttgca ggtggatcaa ccgcttatct gggatacgtt
     1561 tcaattcttc taccatagga gcaaagcgcc ggttgaaacc gatcatcaaa attatttggt
     1621 tcttatcagc taattcttgc aattcacgaa cttcatttag atggacactc agtggtttgt
     1681 caatataaac atggatacca ttttctagac attttttggc taattcataa tgtacttttg
     1741 ttgctgcgtg gatcatacat gcttcgattt tttcttctaa aagctcatcg atcgtttcat
     1801 atagatgttg gaacccgtac tctttttgaa ttctttcttt tgtttcttta tttcttgttg
     1861 caaaataaaa atcaagtgtt ccttgttttt tcgcataagt tggtaaatat gctttttctg
     1921 cgatattccc tacaccgatt acaccaatct tcatctactt tcctcctgga tcatttagtt
     1981 actttcatct tacaccattt tttactaaaa tgaggtacaa tagtatcgca aaggtaagga
     2041 aattaattcg aaggagtgtg cttttattga aatttatctc ttggaacgta aacgggcttc
     2101 gtgccatcgt aaataaaaat tttttagaag tttttcatga gttagacgcc gattttttct
     2161 gtctgcagga aacgaaactc caagcaggac aaattgattt agatcttcca ggttattatc
     2221 aatattggaa ttacgcggaa cgaaaaggct attccggcac agcgattttt gctaaaaaac
     2281 cagcattgaa tgccacttac ggaatgggta tcgatatcca tgatacagaa ggccgtctga
     2341 ttactttgga atattccgat ttctttcttg taacctgtta cacgccaaat tctcaaagcg
     2401 aattaaaacg tttagattac cgattagaat gggaagaagc cttttataat tatttggaaa
     2461 atctaaaaaa acaaaaacca gtcatcgtgt gtggggatct aaatgtcgct catcaaaaga
     2521 ttgatttgaa aaactggaaa acgaatcaaa aaaatgctgg attcactcca gaagaaagag
     2581 cggctctttc tcgactgttg gataacgggt ttatcgatac attccgttat ttttatccaa
     2641 cccaggaagg tgtctactct tggtggaact accgattcaa ttcaaggaaa aacaatgctg
     2701 gttggcggat cgattacttt ttgacgagca aagatttaga gccaggacta gctgatgcaa
     2761 agatccatac ggacattatg ggcagtgatc attgtccagt agaattagat ttgaaataaa
     2821 gaggttgtga aaaagtcgtt tagtttcgag tattaagaaa aattttgtga aaatggcttt
     2881 ccacattttg cataaatttg gcataatacc gagaaactgg cttttgaaca ctgtttatcg
     2941 agagattgtg aaaaagctgt cctgcatcaa gtaataagaa cagccaaaca aaaatagttc
     3001 ctcatatttt tcgtttggtt gggcttatta ccgcagatac aagcttttga acaccgttta
     3061 ttaagaggtt gtgaaaaagc tgcactgcat caagtaataa gaacagccaa acaaaaatag
     3121 ttcctcatat ttttcgtttg gttgggctta ttaccgcaga tgcaagcttt tgaacaccgt
     3181 ttattaagag gttgtgaaaa agctgcactg catcaagtaa taagaacagc caaacaaaaa
     3241 tagttcctca tatttttcgt ttggttggac ttattaccgc agatgcaagc ttttgaacac
     3301 cgtttattga gaagttgtga aatcagcgtc ctgacctgag tattagagaa acaaatggca
     3361 aaatagttcc tcatattttg ctattttttg agctaatacc gagggtcagc tgattgaaca
     3421 ccgtttagtg gagaagttgt gaaaaagtcg cctactttcg agtcgtaaga aatttttttg
     3481 aaaatggttc ctcacaactt tgcataaatt tgacttaata ccgagaagtc atgatcccca
     3541 tctaactcca aatcacaagg aatgagttca aaaactagct tttctcaaca aatattacat
     3601 aaaaaattct atgttttttg ttaaatttat ttgctttttt caaaagtatg ttacagtaat
     3661 ttcccaagga ggttttacag aaatgaattt tgttgccatg gattttgaaa ctgccaatca
     3721 tcagccttat agtgcttgtt ctcttgcgct agtcatggta aaaaatagcc agatcgttga
     3781 tgaattctat acattgatac agcctgagac tccttttttc tggcgaaatg tccaaattca
     3841 cggaatccat caagaagacg tacgcaatgc gccgaaattc ccagaagtct ggcaaactat
     3901 ccaaccatat tttcaaaaaa atcgtttagt cgttgcacat aatgctgctt ttgatacaaa
     3961 ggtcttagcg ggatgcttag attattatca tcttgaacag cctaattatc tttctttatg
     4021 tacagtgaaa agtagccgtc gcctgtttcc tgaatttcca aatcatcgat tgaacactgt
     4081 gtgcgaaaat ttgaacatcc aattagatca tcaccatgat gcattggaag atagtcgtgc
     4141 atgtgcggaa atcctgcttt atcaagaaaa aaaattcggg acagatccat tgaaaaaact
     4201 tgtgactata aagtaaaaga atacttatga aacacacaaa gaggtcgtga gcctgccgtt
     4261 tcactccgag tcacaaggag caatttcaaa aaatagcttc tcatgtttat taaatattgt
     4321 gactggtgac cgaggagctg gcaggagaac accgtttatt ggagaggttg tgaaaaagct
     4381 gttctgcatc aagtaataag aacagccaaa caaaaatagc ttctcatatt tttcatttgg
     4441 ttggacttat taccgcagat gcaagctttt ggacaccgtt taccaagagg ttgtgaaatc
     4501 agcgttttga cctgagtatt agagaaacaa acggcaaaat agttcctcat attttgccgt
     4561 tttttgagct aataccgaag gtcagctgat tgaacaccgt ttattcaaaa aaaatggact
     4621 gcgacacgac ttttgtcaca gtcccttcgt gtgtttttct gccttctcaa aaggatgaca
     4681 gtttctcact cttttcctat ttcttctatt caatcaaata actcatttta tatacatcta
     4741 aaaattcacc tagatctgtt cttgcccctc taggcatgac cgcttcgatt tggaagccca
     4801 tttttcggta aaggtgtacg gctctttcat tacgaacttg aacgtcaagc tccaatcgaa
     4861 aaagtacacc catttccttc gcccaactga tgatttcttc tagcataaga gtgcctagtc
     4921 ccattcccca atattcctgc agaatactga taccgacttc tcccacgtga gacaaacgaa
     4981 attgactgtc tgccttgaca gatgctgttc caataatcgt cccttcatag attgcaagca
     5041 gcaataaatt attattgctt tcgtatatat aatccaactc ttcttctaag gtttctggtg
     5101 gtaacaggag ttctgcttca tccaaaacta aaaattctgt ctccttatta acttgtccca
     5161 tcatcaaaag aatgtctttt gcatctcctg gcactgcttc ccgcaagatg atcccaactt
     5221 cttcactcat gattttcagc ccatttttct agaattcgct gtttgaaggt ttcactttgc
     5281 tccccgtatg agattagttt cacataccgc ttttctaaat ctgtatcact tttttctaaa
     5341 atcagttcca aataatcctc cggcagtgct tcttctaata gatttcccca ttcaacaacg
     5401 gaaagcccat caccttcaaa atattcatct aaccctaaat ctgctccgct agctgctacc
     5461 cgataaatat ccatatgata caacggaatc cgaccttgac tatattctct aataatcgtg
     5521 tatgtcggac ttttgatcat ttgcgttatc cctaatcctt gggcaattcc tttcgtcaac
     5581 gttgttttac ccgcacctag atcaccagtc aatatcagat tgtcacctgg cattgcggaa
     5641 tgaccaattg cctctgcgaa ttgttctgtc attgttaatc ctgatagttc gatcattttt
     5701 catcttctcc cgctcttctt tctttttgcc agtatatcgc gtaatgaagt ttttttaaag
     5761 aaaaaatgaa ctagtcaaga acagcagctg ctgtttttgg ctagttcgcg tgtatttgtt
     5821 aatcaatcaa tgtttgtgct gctgtgataa tcgataattt atatacatct tcttcatttg
     5881 ctccacgaga caaatcagaa accggtttgt tcaagccttg tagaattggt ccgattgctt
     5941 caaagttacc gaaacgctga gcaattttat agccgatatt tcctgactgc aattctggaa
     6001 atacaaagac tgttgcttga ccagctactt ctgaatctgg cgctttcagt tgtgcaactg
     6061 aaggaacata agaagcatca aattgcaatt cgccgtcgat catgtattgt ggcgctagtt
     6121 ttttagcgat tttagttgct tcaactactt tgtctacttc tggcgatttc gctgagcctt
     6181 ttgtcgagaa gctcatcatt gctacttttg gttcgatatc gaacaattct gctgtttttg
     6241 cagaatctac ggcgatttca gccaattctt gcgcagttgg attcacgttg atcgcacaat
     6301 ccgaaaagac atatttttct tgatctcttc cacgtaccat gatcatcgct ccgcttgtcc
     6361 gactgatgcc aggctttgtt ttgatgatct gaagtgctgg gcgaacagtg tcgccagtcg
     6421 aatggatcgc tccagagacc attccgtctg caatccccat atacgtaagc atcgtaccaa
     6481 agtaattcac atcttttaaa atcttttctg cttgttcttt ggatgccttt ccatttctac
     6541 gttcaacaaa agtatccacc atttcatccc acttatcgta attatcaggg ctgatgatcg
     6601 taaagctaga tgttttgatt cctctggcat gcgctgcatt tcttatttct tcttcgttcc
     6661 ctactaaaat gggttccaca agttcttctg ctttcaaccg ggcagctgcg cctaaaatac
     6721 gagcatcagt agcttccggg aaaacgattt tgatcccacg gcgaacaatt ttaaatctta
     6781 aactatcaaa caattccaca agatgacctc cagacgtttt tatatgtatt atcatacact
     6841 attccagaaa aaaagaacag tttattttat ctgtatcagt caaaaatgct ggaaaaatta
     6901 agtttttcac atacaaagcg aactgcacta taacaggaaa gcgctgtttt attacactgt
     6961 ttcaggcagt tgccaatcaa tcggttcttc tcctaacgca aggagcgcat cattcgtttt
     7021 cgagaagggt ttggaaccaa aaaagccacg acttgcagat aacggactgg gatgtggaga
     7081 tgttatgatg atatggcgac tcttatcgat cattttgatt ttttcttgtg ccggttttcc
     7141 ccacaagata aacacgattg gtttttcccg ttcatttaat ttttcaatga tcgtgtctgt
     7201 taatctttcc caacctttcc cgcgatgaga atacgcttgc ccttcccgaa cggtaagaac
     7261 ggtgttgagt aaaaggactc cctgtttagc ccatgacact agatttccat gttgaacagg
     7321 tgcgattcca agatcatcat aaagctcttt atagatattt cttaacgatg gcgggatctt
     7381 tacgccaggt tgcacagaaa aagataagcc atgagcttga ttttctccat gataaggatc
     7441 ttgtcctaaa atcaccactt taacttcttc ataaggagtc aattccaatg cttcatagat
     7501 atgatacata tctggatgga tcttttggga cgcatattct ttttttaaaa attctcttag
     7561 atgcagataa taatcttttg aaaattctga ggacaaaact tcttgccaac tgttatgaat
     7621 gatttctttc aaaaactcca cctcctaata aaaagaataa ctcacatttt cttcaatcac
     7681 aagggacaat cttcaccctc agcacttgat taatatggta aactaggaac aagaagcaga
     7741 aaggaacgat aatcattatg atcaaattaa ttgcatcaga tatggacggc acattattag
     7801 atgcccatat gagtgtttct cctgaaaatg ccgaggcgat acgttttgca aatgatgccg
     7861 gtgttgagtt catggtcgct actggtcgaa actatcaaga agctcgcgca gctttagatg
     7921 aggtaggaat cgattgtgcg atgatcactt tgaacggggc acaggtattt gataaagaag
     7981 gaaattcatt gtttaccgtg tcgattccaa ataatcaagc aatctctgtt ttagatattc
     8041 tagatgctaa cggtatttac tatgaagtag caaccaacga tggtctttat tctgaaaatc
     8101 aagcgaaacg aattgaaagt tttgcttcca tggtggctac ccatttacct caccttacgt
     8161 ataaaatggc gattgccatg gcctcagcta agctggagtt attgcacatc acatacgtgg
     8221 acagtattcg tgctattttg gaagaaaaga atctggaagt tttaaaaatt atttgttttc
     8281 atacagaagg gccagctgta ttaggtccag ttggaaagca gatttctctt ttaggcgatc
     8341 tagccgttac ttcctctggt caaaacaata tcgaagtgaa ccataaaaac gctcaaaaag
     8401 gaattgctgt tgcccatgtc gctcacgagc gtgggatttc tttagaagaa gtcatgacga
     8461 ttggcgataa cttcaatgat gtcagcatgc tgcaagtagc aggagtcagt ttcgcaatgg
     8521 gaaatgcaga aatcgaagta aaagattatg ccaaatatac aacggacaca aaccttgaat
     8581 ctggcgtagg aaaagccata ttgcgcgcga ttgcagaaga cttgtagttt tatctagaaa
     8641 agagggatat agatgtcaga atttttcatt caggaaaagc agcttggacg tgcaactcgg
     8701 acaattgtca aaaacgaaaa aggacattcc ctgtttttga tggttggtcg ctggggaaca
     8761 aagggcgatg cattgtctct ttatgccatg aatggcgatc tcgttgctca catcaagcag
     8821 ataagcctga cattcggtac gcgttttgag ctgtataaag agttcgaaaa ggtgggaacc
     8881 atgcagaaaa tattcaattg gcccggtgac ttttattata ttcgtagact tcactggaca
     8941 gtccaaggtg atatctataa tcatcaatat caaatccatc atttcaatca gcaaatcatg
     9001 aaaatggata aagcaacact tttttcagga gattactatg tattggatgt ctcaaatgat
     9061 gaagatgctc cagtttgtat ttgtattgct gcggttttag attactggct ctaccgtaaa
     9121 gacaaaagtc gcgataagcc tgtttcttac cgacttaact taagttaaaa agatgtacct
     9181 gaaatgcgga aatacgcatt tcaggtacat ctttttagat attcttttgc atatgatcat
     9241 aaagatgacc actgatcatc cgctgaccga caactttgaa gtcatggcgt tcgtataatt
     9301 ttttagcctt tggatttttt tcatccacac ttagaccgat gacggagcga ttagctttct
     9361 tcgccaattt aggtaatgct tctaataatc gcgaaccgat gccttgtcct ctaaaatctt
     9421 ctgatacact gatagagtcc aaataccatt cgttgggaaa cgtttcttta tcaatgaaca
     9481 aacgtgtaga cgcatctagc tgatatttct ctaagatagg taccagtggc tgatcgatga
     9541 ttgattcttc ttcatctgta taaccgtaag caactcctgc aactttaccg tctatttctt
     9601 ctacgatacc tcgtgaataa ctgtaacgat aatttggatc agtgattgct tcttctaaaa
     9661 tctctaaagt cttttgttcc cctgtctgct tcaaaaatgg cagttccata tcttttagaa
     9721 tcactaagat caatggggcg atttctggag catcttcttt tctcgcaaaa cgaatcatag
     9781 tttcctctcc ttttctccat tctaacatgc agaaaaagaa atcgcaaaat cgttatttta
     9841 aaaaatcttc aatcttttca gtaatgaaat aaccatgagt aacgattctt gccgcttcag
     9901 ttcggcaata agggcagacg actggcattt ctttatcatt atgttcgact acattattga
     9961 cttcttcgta tgcaaatttt tttccgcaat tagggcattt cattttttcc atgttatctc
    10021 aacttccttt catctttact ctcttagctt actcttatat tgtaaaaaag agaagcaata
    10081 tccttcttct atgcagatat ccatacctga cggtcctgtt ctccatctat taatcggact
    10141 ttataataag gacgttcatc tttgactaga agtgtccact ctttcacttt tgcttttttc
    10201 ccgtagtagc tgactgcttc cttcatcgct gcttgcggtt gtttgaactc ttccgttttt
    10261 tctatatcaa aaagatgttc tgcggccttt tccgtgtcat ttcgtacggt tgttttccca
    10321 gtggatggat tgactgctat gacttgtcct tcacaaaaaa actggtattc atatgttgca
    10381 ttcgtaggat tctccaaatc tttttcaact gcaaaatgaa tagcagatat gttatgttct
    10441 tttgtttcgt tttttacgat tgctgctgct tttttcaatg agatccgata ttcctggtca
    10501 ctaatatcat ccatgtgaaa agcggcattg agcagtcccg tcataccggt aatccctatt
    10561 acgatgaggg cagcaacacc aaacatcatt tttttcattc ttcattctcc tgtttctttt
    10621 ttttaaagaa tatcaagaaa tgaacgaatg aaaaatcgaa tgaaacgaaa aaaaagtgac
    10681 aattttgtta tctttattcg tgcagttcta atggcaatcc gtctggatca aagaaaaacg
    10741 tcattttttt tcctgtaaag gtatcttctc taatcggttc tgattcgatc cctaaggact
    10801 gcaattctgc aattgtttct tcaatatcgt ctactttgaa agcaagatga cgcaagccac
    10861 aagcttctgg atagtttggt cgttgtggcg cattagcaat tccaaaaatc tccaattcag
    10921 cacttcctag tttcagatct aatttatagt catctcgttc tttgcggtaa ttttctctga
    10981 ttacttcaaa tcctaattta ttaacataaa aatcacgtga tttctgataa tctgaaacaa
    11041 tgatggctat atgatggatc gttgtaaggt tcatatgagc actcctattc ttcgtttaag
    11101 ctcggatcta tattaatctc cagttccatt gtaaatgtgt cagattgaaa aaaatagctg
    11161 tttttccttt ttataagaaa aaaggcaaaa aaaacagaaa gtaggtcctc caagacactc
    11221 ttcctgttcc caatataata aatgaaagta agaaattata ttttcaagaa aacaaacaga
    11281 aaattatcat aacacaaatt atttataatt gaaagactag ttttttttca agaaaagcat
    11341 acaatagatt gtgttagtaa atacagaaca cactacattt agtgtcttaa ggagtcctca
    11401 tacatgaagt catattttca gtttttagct catcagctaa aaggctggcc gcaacaaaat
    11461 tattatcttt tctttttcag tcttggctgc caggtcatga ctcttgtcaa tagtcccatc
    11521 acttggctgt ccgttattac atttatcggt accacgcttg gtgtattgtg tattttatcc
    11581 atcaatgcgg caaaatcagt caacggtatt ttaggtattt tgtctgcgat ttgttttatt
    11641 atcgttgggt tttcagcaaa gaactattta agtatcggtg aacagattgc ttatatgatc
    11701 acattggata tccccgtatt gttaagtaaa gaatggaatc ataatatggc ttcaaaaatc
    11761 cgaaaattca ctggaaaaag ctggtttatc gcaatcatca gtactttcct tgtctatctt
    11821 gtttcaggat tcctgattgg tcgttataca gatgatcctc ggccgtgggt agatgccatt
    11881 gctttttcta ttagcttgac tgctggtatc atcagtttct tgcgttacaa caaccaatat
    11941 tactggtggt tagcttcagg gcttgctcaa atggtccttt ggtatatttc atttacgcat
    12001 ggatctgcaa gtttggcgat gttcgtgaac agctctgttt acttgctgaa tgatgtcctt
    12061 gcatttaccg tttcaccttg gtataaccaa aaagaacgtg aacgtatgca aaaaatggaa
    12121 gaagaatact acaacgcaca acaacacgca taaaaaaact gagcgtggga caaaagtaaa
    12181 gaacactttt gtctcacgct ctaaacacgt ataaacggcg gaatcagaag caactccttc
    12241 ggaaataagc tgaaattcac aaaaatttga aaagcaattt tcgtgaattt cttcttattt
    12301 cttggagtta aacgcttctg tcccagcctc tatttcctac gattagtcaa gtaaaaggtg
    12361 caagaaaaat agctcgtaaa cgaaagcgac aaaatcaaag cacaccaaat aaagccccgt
    12421 tcgtaaaaaa ttttcgatct gggcaaagaa gtggtatact gtaagtggtt tagttaatgg
    12481 tccactgaga ccgcgtacgc atagtcgtac tctgtatgtg ccctaaccat agcgtgcata
    12541 catttcagaa gcttgttcat acaagcaact gtggcaacct tatctttctt agggataggt
    12601 tgctttttta atttgtagta ataatcgaca atatgattag gagctgcgcg ttgctggcga
    12661 atcatatttc gaatggtgaa gaaaataatt ttccgtgctt tgggattacc acgcttattg
    12721 atgcggtctt ggccagtata cttacctgaa tgataccgtc ggatgtcgat tccgacaaag
    12781 gcattcaatt gtttatgggt tttaaatcgt gtgatatctc ctaattcacc gagcaataaa
    12841 gcggctgtga gtgcgccaat cccagggaac gtttggtaca acaaaaagca aggagagcct
    12901 tcggcttttt tgatcatttg tgtcgccagt gctttttttt gttcgagtaa atcttggaga
    12961 agttcggcgt aatagatcgt tttttgacag tggatactgt ctttttcaac agctggatag
    13021 gcacactgtg catagtggat gatttgatct gccttttgct cggcacgatt cgcggagatt
    13081 tttttccgtg tttcgttgat cagtttattt tttatttttg tccgagtaga tgccaataca
    13141 aaatctggat ggggaaaaag acggatcaat gtcaatgcgt aaggggtcag cctgtttgaa
    13201 aagaactgtt caagctctgg aaaagtcaat tgaaggcagt tgtgtaaatc catgcgcaga
    13261 cgagtgattt tcttctcaat ttcttgataa aaacgggcta aatcgcgcat ctctaagtaa
    13321 aaattgcttt gtacttcttt tggctgacgt tgatttttaa gatgagtctg tgcgagccgg
    13381 tgggcatccg atttatctgt tttccaactg cgtaaggtgc tttcttccat ctgttttttc
    13441 gcctctaacg ggtttaacaa acagtaagaa aagtgatgat cttgaaagaa cttttcgact
    13501 ggtctggaat aaatccccgt tgcttcaaaa acaatcaatg gacgctcagg caagtgctgg
    13561 atctcttcga gtaatgcatg aaatcctctt tgagtgtgtt caatctcgtc ttcaaataag
    13621 caggtgtcgt ctttgtatag gacactgtag ctgtgtccca ttgaaacatc taatgcaaga
    13681 atgtaaggca tttgttttcc tcctttttaa gttttgaaag aactttactg cttcatttcg
    13741 attccaattt cctatacacg ggctcatggc cccaacatac gtaaacctga ttctaaaatg
    13801 acagtaaaga agttcggttt ttgataggga ctcgtagtcc tcaaggcttt gttcgaactt
    13861 tctttcactc ttcactataa tctaaaaata gaaaatagag gagaacatca ttcgtcaatg
    13921 atgttcccct ctaatcttag tatgtttttt atagtaattc cttcagatac tttcttgcct
    13981 catcattgaa acgcagtgat tcttctaact tctgataata ttccaacgct tgtttctgag
    14041 cttcagtaaa acgataatgt tttcctttcc atttgtcgat tgtctcttgc acatttccag
    14101 attggatatc tttcactact agttgccatt tttcttttga gactgtacgg tctttcttat
    14161 cagcagattg tatagcttct cgttctttac gcaatacttt taaaaaacca tttctgacta
    14221 tccgaggaat gctgtcgaag aacccttctg taaaaccata gcgacaatat tgcttccaaa
    14281 taaatagata gatttttggt tggatgaatc gttggttttt cgcaaaatat tccatctgag
    14341 cagtgacttc tggtgttcgt tgacgttttg tctttctcaa cacggaaata aatgtttcaa
    14401 aataagcatt aggatgtact ggcacttctt catatacgat ggattgccaa tctttacgta
    14461 aaaatgcgcg atagttgatc aagaattctt ctacatcctt tactaattct tctgcaaagt
    14521 cgtcaaatag ttcttttttt atctgtcgct tttttgactg cccattgatg tgttcactta
    14581 ttttcttgat ttcattttcc aatgcttgat aatccgctat gtaaatcggc atttctactt
    14641 ggcgaaattc tgcgagccgc tgagctaatt ttatatccgg taaatatggc tgtttcatcc
    14701 gacgattttg gtacaagaca aaacagtatt tttgccatag tccttcagga aaatcgatat
    14761 tcttctgctt aagccataat aattcgtcta aggccagatc aacttttgtc attccctgat
    14821 ggatcgaagc tgccactgtc ggagagacat gcaggtgatc ggcaaagtgg ttgatgccta
    14881 gttttaggat ctttccggtt tttggcgatt taacgatgta ttgcactttt aattctctac
    14941 cacactcaca atacaatttt tgctggcttc gttttgttcg aaaaaacgga ttgatcttga
    15001 agtcagaaaa cacccaatct gttgcagcta aataatgttc ttccataaaa cgagagctga
    15061 ttagatatcg gcggtgctta tcgactagtt cccgctgttt tttcgttaat tttgcataga
    15121 tctcccaacg ttgactgctt gtgtattgtt tagaaatttt gatgggatag cttttgctga
    15181 tttcttttgg aaaccattct tcaaagacgg attgccattc ttccacggct gtttctttcc
    15241 tttctaatta caaaaagctg gctactaaca taacgttagc aaccagccag tgtttcttgc
    15301 taaattagtg agttgttccg gcatttccgg aaatatcttc tttattttct actgctgctt
    15361 caactgcata agtcaaagaa gaagcaatgt cttctaaaga catagaaggc gtacccactg
    15421 gacgatcgat cacttgatcg ggtgagaatg gcacgtggat gaacccacct cgaattgccg
    15481 ggaattgtgt ttcgatcaaa tgaagcaaac gatacatgat atcattgcag acaaatgttc
    15541 ctgctgtata agagatgtac gctggtagac catgatcttg tacatttttt gtcattgctt
    15601 tgattggcaa tgaggtaaag taagcggctg gaccgttttc ttctaaagcc gtatcgaatg
    15661 gctgatttcc ttcattatca gggattcttg cttctgctag attgattgcg acacgttcga
    15721 acgagactgc tgaccgccca cctgcttgtc cgacacagat cacgacatct ggttggtgct
    15781 gttggatggc ttctttgact acttgactgc ttttttcgaa tacggtcggt acttccactt
    15841 tgatgatttc agcaccgctg atttcatctg gcattttttt tactgcttca taggctggat
    15901 tcactttgtc cccaccaaat ggatcaaaac ctgttactag tactttcatc tgttgttcct
    15961 ccgtccgatt gatttaacat atggttatct tttatcatac cattttcctt gaacagcgta
    16021 aataaaggaa acaatagttt aaataacata acagatcaaa cttaatatat tcttataaaa
    16081 aaatagtgtt ccactacaat gttagaagag gttgcgacaa ttattctgtc acaacccctc
    16141 tcttctatta tttaaataga atcatataag caatttggaa aactagcata aataacgcaa
    16201 taaacaactg gtttttgatc actccgtatt tgtctttcat ctccagcatc gctacaggca
    16261 cgatattgaa gttagcagcc atcggtgtca gcagggtccc acaaaaaccg caagtcaatg
    16321 caaccattcc tattaacgct ggattcgctc catgactaaa tacgaatggt gcaccgatcc
    16381 caaccgtcat cacggtgatg gccgcaaatg cattccccat gattaccgta aatatcacca
    16441 tgccaagagc gtaaatgatg atgccgatgt tcacatttcc ttctggaacg atcgtaccta
    16501 ctgcattgga aatcactgtc ccaactccag cgcttgtaaa taccgcacct agagaagcta
    16561 aaagcattgg caacatgctt aacggcccta ctgtgcccag catatctgct gcatcatcta
    16621 aaaatgtact tggtcgatta tctcgagaaa agaacatcaa gattagcatg gctgcaaaaa
    16681 caccgacgcc cacgccgact aaagcaccta aattggtaaa tagagcaaac aagatcgcaa
    16741 agacaccgat actcaaagct ggaataaaaa ttttcattcc tagtttgtct gacatttttt
    16801 ccatatattc ttttgatgga agccggcttt ctcctttgct gactttctta gcaattgctg
    16861 gtatggtcat cgcaaagatc aagccaccgt tcacgattgc cggaagccat cttcctcctg
    16921 caataactac ccctaagacc gtccagaaaa aggctgaccc ataacgatga ggattggttt
    16981 tatctgttaa atttcttact ccagtgtaaa tcaatacgag tcccatcacg atataaagga
    17041 tctccagtaa tttatcgcct aataaaatat caggatttgt aaaaaagtcc atcttattgc
    17101 ccctttcctg gataatattt cttcgctaat ttcttttctt taagatagaa gtagacactt
    17161 gccacaacta aagccgtgat agccactgga acttccacta aagcaagctg tcccaatgtt
    17221 acgtcatatc ctagatcttt cagtgttgat tggactagca acgcccctgc tcctccgatg
    17281 aatagtactt ggccaaagaa ccagcagaca ttttccatag ctgaagccat accttttagt
    17341 tcttcttcat gcttaggatg gacttctaac ccttttgatt cgatggtccc caatgccatc
    17401 ggtaaaatga ttggacgaac aaatccagca actccaccaa aactcacgtt aaaagccgca
    17461 aagatcattc gaaagatccc ataaatatct acaatcatac cagcagatac tttcttgaat
    17521 cgcttgatca aggaagccgc tgattctttc agaccatttc tttcaagcgt tcctgttgcc
    17581 aacataatga tgataaaaat cgccatcccg cgattgttta caaaacttgt acctaaaagt
    17641 tctaacattc cttgaatgcc aagaccgcca gtcaaagctg taacaactaa agcaaccatt
    17701 acgatcaata tcgagtctaa tttaaatgca aacccgagaa taacaatcaa gacgcctaaa
    17761 aggctaagat aattttccat atattttcct ccataaaatg acaatatttt tttaattata
    17821 aagcaaaaca cataaaaaat atagttgttt tctcacgaaa tagacactat ttttaaatat
    17881 ttttcgttat ttttttatca caaaaattat cttacttatt gtaaggtaaa gcttttttta
    17941 atttattgcc cgataatata tagaaataag gtctagttac tgtttttttt aaatgattaa
    18001 taaataacaa cttctttcta tgaaattgca aagcattcat cttcttttcc gctaacgata
    18061 tatccgtcag tgcttggtac actttactaa aaagtatgag attgccgata tctggatagg
    18121 tgtgctatac tacagaaaaa gatatacaat ctgccaccgg gtaggataaa gaggatgatc
    18181 attccattag gtctttgaag ggatgatttt cctctttttg tgtatttttt tatagttaag
    18241 gaggaatgta agtatggcat ggatggaatt aatcatagca ggaattttgg aagtattctg
    18301 gtcaacgatg atgaaatgga gtgatggttt ttctaaaatc aattattctt tttacacagt
    18361 gattggcatg attgctagtt tttacttttt gtcaaaggca atcaaaagtt tgccgatgag
    18421 tctctcttat cctatctgga cagggattgg cgcagttggt tcggtcataa ttggtgtggt
    18481 cttgtttcat gataagctga atctttccac ttggttcttt gtggggatgc tactcatcag
    18541 tatcattggc atcaagatta ctagcggcca ctagacactt ggaataagta ctgatcagaa
    18601 aaaataagcc tttttattta atattcaatt tttttatgaa aaaataagaa ataagtccaa
    18661 tgacctagcg acttctccac tcatcaggtg tatgcttgta ctatctatag aaaaggagtg
    18721 aatatttttg aaaagtcttt cggtccaaga attatccaag aactttggtc acaaacaagc
    18781 cctcagcgat gtcagtttta gcttatcccc tggcaaaatc gttggattag taggtcctaa
    18841 tggcgctgga aaaacaacga tcatgaaagc tattttagga ctgatccatt actcagatgg
    18901 aacaatctgt attggtgaaa atgagatttc tccctctagt cataaagggt tggaacaagt
    18961 aggagcattg atcgaatacc cgggtatcta tccattctta acaggctatg atcatctgaa
    19021 gttattctct gaaaccaacg atgtatctgc tatcgacact atcgtgaatc agctcaagat
    19081 ggaaaagtac attcacaaaa aagcgaaatc ctactcgttg ggaatgaaac aaaaattggg
    19141 aattgctctt gccttactga accatccgtc tatcgttatt ttagatgagc cgatgaacgg
    19201 tttggatcca caagcaacaa gagatgtgcg aaaaatcatt accgacttgg ctactcaggg
    19261 cacctctttc ttgatctcca gtcacttact cagcgagctt gaaaaaatgg tcgatgagct
    19321 gatcctgatc aatcaaggaa agatcatcaa acaatgtaca atggctgaat tgaaccaaac
    19381 agcgcaagat ttcatcgtta tctctacaac aaatgatcta gctgctcgaa cgatcttgac
    19441 agaagctgga tatactttgg aagatcctga taaaatcaag cttttaaaag aaaacgaaga
    19501 attattagga accgtgatcc agctattgtc tgcacaaaat atccatgtga cagacgtcca
    19561 gcatctgacg aatgatttgg aatcttctct attggaaatt ctggattcag taaaggggga
    19621 agaagaaaaa tgacattgct tgtaaaacaa gaattattta aattgatcaa gaaaaagtca
    19681 actgctgttt tatctgttct attagttgtt ttgctcatag gaacagcact tctagccaaa
    19741 aaatacacga cgatcattga tccagttgaa atgactgctc agctttttag tgccacatct
    19801 tggattgtct ttatcatgat tgcagctgca agcacgatca tttcgatgga agcacaatat
    19861 ggtacattga aaaatctcct ttatcgaaag tattctcgag gagaaatatt agtaagcaaa
    19921 tggatcacat tagttattta ttctgtttat ttataccttt tagctattat tgtcactgtg
    19981 ttgatgaaac tgattctttt cccttcgatt tccttcacgg agaaggtttc tactggtcaa
    20041 acactgatcc aaagcttgtt cacttatact ttgggtagtt atatcggttt atggctgatt
    20101 ttaagtttag tgttgatgat tgcttgcttt atcaatagtt ccggcgcatc catctcagca
    20161 ggaatcgtct tttattttgc ttcgtcaatc atatctggta tccttattgc actcatccaa
    20221 aaatgggagt ggatcaaatg gaatccgatc agtatgttga atctgcaaaa ccaaattgga
    20281 aacgaagaga tcatgaagca attgactcat ctttctacta atcaaatgct attcggtaat
    20341 ttagcttaca tcgttttgtt cttagcatta ggatatctcg tatttaagcg aaaaaatgta
    20401 tgatgactca tcaaaaaaga ggctgtgaaa gagacgttca gccttattgc cgattggctg
    20461 tctcttaaac accgtttatt ctgaaaaagg agctgtgaca acatttgtgt cacagctcct
    20521 ttcctagtat gtttttgaat aaacgacagg ctcacaacct ctctttatac ttcttttata
    20581 cttctatacg tgaagcatcc cagtggcaaa taaaaccatt gcaatcactc cgaccatcgt
    20641 cacacctata agtaaaatct tatctaagtg gatatgttgt acaggctcgt tttgctcctt
    20701 acgtgctttg aggtaaaaat aaatgcctgg caagaaaaga acaagaacaa gcattacttc
    20761 attaaaggct acgactagca tagcccatac ttgaaaggca cttgctaaaa gccctactaa
    20821 aaattggacc cattctttcc gctgataaga aagtttcatt tggtacaatc ctaagaaaag
    20881 ccacgtgaag aaaatagctg ctgtaccaag tgtgtaagca aagttataag cactgtcagt
    20941 gaataataat gaaagtaaaa agacattcgt acataacgtc gtcaatacga tagcataagt
    21001 tggtgcccca tttttgttga gttttcccca tgctttaggc aaaagtccgt cttcagccat
    21061 gtttcccatt gtttcaatcg gtaagatcgt ccatgagagc caaacaccaa ctaaagaaat
    21121 gatcaaaccg ccgttgatca cgactgctcc ccatttacca accatcattt caaacacata
    21181 gcccatagca ggctgcgcct tgatcgtttg cagctgattc atcgtcattg tcccatatgg
    21241 aagcatggaa accaagacgt aaataataat caagccgaat atccctagaa tcgttgcttt
    21301 cccaacatct gattgttttt ttgctcgtga acctacgaca gaggctcctt caataccaac
    21361 aaatacccaa agcatcacca tgatgcaact caatacttga ctggaaaacg ggatcggctg
    21421 tccattcgca ttcaatataa tgtcgctaga aaagttatca atgaattgtt ggatcgaaaa
    21481 cgtgctgata gctactacaa taaataaaaa taatggcact aatttacaga atgtgacgat
    21541 agcattgacg aaactagcat tttcgactcc ccgattcact agccatgcat atccccataa
    21601 aaagactgta ctcatcacga tcgatgccag attttgccca ccttcaaata ctgggaagaa
    21661 atatcctaat gcgctcatca acaatgttgc aaaagctacg ttgcctaacc agcatgtgag
    21721 ccagtaaccc caaccgctga taaaaccagc gaaacggcca aaaccttctt ctgcgtaact
    21781 aaagatcccg ccttttaaat ctggacgttt tttattgata ttattgatcg acaaggagag
    21841 catcaacatt gctgttccta cgagtaccca actgaaaata gctgggccgc cagccgatcc
    21901 ggagatgtcc gtcataatac caaatacgcc acctccaatc gctgaaccaa ttactaaggc
    21961 agcaagtgga attaaaccaa ttcctttttt ctgttgctct gtatccatgt aagcatcctc
    22021 caaaataaaa ccgcttttat caaaaagtat aacaaacctt ttttattttt acttaattaa
    22081 taaaaacttt aatagaaata taattctacc ggcttttttc tcattataca agcaaagcgc
    22141 ctagtccctt tccaaattac accaccaaaa aacttaaaaa agatgctata ctaaaaggaa
    22201 agaattggag gatgatccat gaacatttta tatatatcga tttcaggaaa tacgcgtgcc
    22261 tttgccaaac atctagcaga atatgctgaa aaaatgcatg cagaagatcc tgtcaatcct
    22321 gaagttactt taaaagaaat ccatgaaaat agtgatttta cgaaagaaac agaaccattt
    22381 tttactttcg ttcctactta tttagacggc ggaaatggtt tagataacgg tgatacagaa
    22441 atcttgaccg aaacgatgcg tgaatatctg gaacatgaag acaatcataa actttgtctt
    22501 ggtgtagtcg gtagcggaaa taaaaacttc aataatcagt attgtctaac agccaaacaa
    22561 tatgcacaaa catttggctt tcctttcttg gcagattatg aattacgggg aacaccatct
    22621 gatgtgacac gaatttatca gattttagca gaaaacaata aataataaaa aagaagaagc
    22681 tgaaaaatag atttcagttt cttctttcgt ttctattgtt tctatcatat taagctttga
    22741 catccatcac aggtgctttt gcttttgctt tacctgtttt ttccaagaca aagtttttta
    22801 cacgatccat attggttacc aagacgatca tggatgtttc tttgcctgct gctttgatcg
    22861 cttctaagtc agcttctgct acaagcgtat caggtgtcac ttgctgccct tctgttactt
    22921 tcacatcaaa tggcgtaccg cctaagtcaa ctgtgttgat tcccatatga agcaagattt
    22981 ctaaaccatt tggcgttgtg atcccaatag cgtgtttcgt tgggaagaca gagctgacta
    23041 ccccagtaac tggagaatag atatgtccgt tttctggttc tacaaagtag ccttcaccca
    23101 tcatcttttg tgcaaaaact ggatcacttg ctttatctaa agaaacaact gttccgtcag
    23161 caactgatga gaggatagtc acactttcag ctgaagtttg aggtactact ttttcaggca
    23221 cttcatttac tgtggctgtt tcagtcgcta attcttcttt ttctaaacgg tctttaggaa
    23281 taccgaagaa gaatgtagca acgaagccac cagcataagc tgccaataat ccaaggatgt
    23341 agcctaacca tttaccgtca gagatcaatg ggatcaaagc ggctcctgaa ggtccaatag
    23401 cgatagcccc gacatttccg atcattccga tcactgcccc gccgatacct ccaccgatac
    23461 aagcagtaat aaatgggcgt cctagaggta atgttactcc gtaaatcaat ggttcgccga
    23521 ttcctaggat cccgactggt aaagctcctt tgatcatttc tgttaattct ttatcttttt
    23581 tgcagcgaat ccataatgcc aatgctgcac ccacttgtcc tgcaccagcc atcgcaagaa
    23641 ttggaagcaa tagcgtacga ccagtttcat caatcatttg cacatggatt ggagtcaaaa
    23701 tttgatgcag tccgaacata accatcggca agaataatgt gcctagaacg aagccggcaa
    23761 atgctccacc aacgccaagt acccagttga tcccaccgac catgccatca gaaatgaaac
    23821 ctgctaaagg catgatgaag aaaatttcaa tcagaccgat ggcaatcaat gaaagagttg
    23881 gtgtaacgat gatatctacc gcatcaggta ctactttatg cagttttttc tcaacgattg
    23941 acaatagcca aacagcgaag atgacaccta ggatcccacc ttgtccagct gcaagagctt
    24001 caccagtaaa taagtttttc agtggtgctt ccggattcat ccctgtcagc atcacaacgg
    24061 ccccgataac tccaccaaga gttggtgttg caccaaaaac ttgtgcagca ttgatacctg
    24121 tatagataac taggtagcta aacatcccgt ttttcaagat gttcatcaca tcgatatatt
    24181 gttgccatgt tgctgcatca agtgttccgg ctgtgactaa gtttgctaaa actgcggcaa
    24241 tccctgccac gatcccagta ccgacaaatg caggaatcat tggcacaaag atattagaaa
    24301 tgtcttttaa taccgatttg attttagatg gtttttgttt cgatttttgt gccgctttca
    24361 tttcttgtgc ccgttcgttt acacggtctt ttccagatgc tgatgaattt gctgtagctg
    24421 tacttacaga agaaagtttt tctcctaatt tcacgcctgc caaatcaacc atttgctgtg
    24481 ctactttgtt cactttccca ggtccgatga tgacttgaag ctgttcatct tctactacac
    24541 caagcactcc tggaatcgct tttaattcag agacatttac aagatcatca ttgatgatct
    24601 tcatacgcac acgagtcatg cagtggacaa tgctttctac attgcccatg ccgcctgccc
    24661 ctttataaat ttgttttgcc agttcttcca aagtcagttc gttttttgcc atggtctttt
    24721 ccccctcata aggtcccttt tacaaatccg tttgcttcag ctaattttgc ttgagcttcc
    24781 tctttatcac tatttgtcaa aatcatgacg atagccagct taacattttg atctgcttct
    24841 tcaaagtact tttcagctgt ttcgtaatca gcttctgtcg cttgcatgat gattcgtttt
    24901 gagcgttcga ttagtttttc atttgttggt ttgacatcga ccatcaagtt attgaatact
    24961 ttacctatac caatcatact gatagtagaa atcatattca agataagttt ttgtgctgtt
    25021 cctgacttca atcgagtaga gcctgtcaaa aattctggac ctgcatcgac ttcgattggg
    25081 aaatccgcgt gtttactgat ttcggcttgt ttattgcaag aaatcgttcc tgtgacagca
    25141 ccgatcgatt ttgcgtaatc taatccaccg atcacatatg gtgtacgacc gctagctgcg
    25201 ataccaataa ctatatcatt ttttgtcaaa ttcagcttga ttagatcttc ttgccctagc
    25261 tcataagaat cttcggcccc ttcgacagct acagtcattg cttcttctcc accagcaatt
    25321 agacctatga ccatttccgg ttctacaccg aatgtcggta cgcattcagc agcatctaac
    25381 acacctagtc ttccacttgt tccggctccc atataaatca gacgtccacc ttttttaaag
    25441 gactcaatcg tgttagcaat cactggttcg atacttgtca gttctttttc aaccgcagcg
    25501 gccactttct gatcttcttt gttcattagt tgaacggctt gagaaatact catctcatct
    25561 aatccaaaag tttcttggtt tctgcgttcc gttgttaggt tctctaaatt gattttgctc
    25621 atttttatct tccttctttt cttataaatt gatagttcat accagctttg atttggttga
    25681 tcaatggtag ctcttctttg ataactttag ctactacatt cactttttca tccgctggca
    25741 aatcatattt ggttaattgg atctcaccca tatagcgcaa atacttttcg ttatctaaag
    25801 taatagaccc tttcatacgc gtagctgtat ttcttgctgg gataggcgga atctctcgaa
    25861 aacgtgcatc tacgcttcgt atgacatctc ttgcatcgtc ttgccgattc gtatgcttcc
    25921 ctaatatata ttcgtaaaat tcttcatcta caggttccgt atggagcaag actgtctgtt
    25981 ctttttggaa agaagcaaat tgctcctgaa cgttttctga cagacctgca tctccgatgt
    26041 aaacaatatc cgtgttactt gctaatagat ctagagcgcc tgcaagagga tggatccctc
    26101 gatgttcttc caaagttggc aatccttgat atagtgggcc tctcaactct gtatctcctg
    26161 gtacaaaacc ttggacaagg agaccttgaa ttttgagcca ttgatttttt ttctgatacc
    26221 attctttgtc cagccctgtt tcgggacgtg gataataatt atgccaagct tcaatctgtg
    26281 agaaatcagc ttctgcttct ttcaattcat cgatatcttt aggcgtgatc gtgctagcat
    26341 tcaaactgat tttcaattga tgtgaccagt cggcaatctg ctgattggaa atatgataat
    26401 ccattcttaa acctgttaca ccaattgctt tcaaaggcgt tagatcttct aatgaaaaac
    26461 ccgctcgttc caatgcctct cctgaaatat cgaccatcaa ttctagttta ttctttttcg
    26521 cttcgcttcc tagttcgatc aatcgctttt gatatgccga aacatcatct tctggtatat
    26581 gcatggaagt gaaaataccg acaaagccat tggatgccat ttggcggata tactgtttct
    26641 tctcttctga caaagaatcg ttcataaata ttgaaaagcc aaacatatta tccctctttt
    26701 tctgtatccg tcttcatttc tat
//
LOCUS       DAZKLM010000032        25824 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3764
            SAMEA115706913-rid22582563.denovo.032, whole genome shotgun
            sequence.
ACCESSION   DAZKLM010000032 DAZKLM010000000
VERSION     DAZKLM010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706913
            Sequence Read Archive: ERR13196647
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25824)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25824)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287823.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287823.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,004
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,849
            CDSs (with protein)               :: 2,849
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 41 of 96
            Pseudo Genes (incomplete)         :: 52 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 15 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25824
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706913-rid22582563.denovo.032"
                     /isolate="VRE3764"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            148..2208
                     /locus_tag="ACG842_001229"
     CDS             148..2208
                     /locus_tag="ACG842_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterial Ig-like domain-containing protein"
                     /protein_id="HFQ0247783.1"
                     /translation="MCSKRRSKQLNKHACRLDKERSCSEKHYVKFMMRTMLISLSVGV
                     YMHVPVQATTQTIKVVRTSVSETTDSPITITHTFTDANGKQLLPALTSYNKVSYPPTI
                     NHYWLIPNKTLWTSETGSSVPLSIFSDMTDPTKGDQLKQITDILNHALTDIKDGGSAQ
                     VSYVYEKDYSQFERKKIDVLAGTKVSISDLVERLKNADGTQGDMSKVISNNGNIVDTT
                     NTGTSTISLSYFDPIALKTMTASAVIHVRADQTAIKGKDLHLRTGESYAPKDLVEYVT
                     KSDGTSGNKEEIKMNGTVDTTTPGEYPVVLTYIDSKTSNVAETTAVVYVEASAASSTV
                     ITHFIDSQTNKELQAETHMSSVNRIPDLRLPAEFSDRVPNIDLSTYEQNGKVTSISDW
                     MQANGLDRKSHWSEILEVLHSELMNNRKQSGGEITFTYVYIPDNRSLEGHDLIVHPNE
                     KINKEELIQSATNSLGKPVPLKDVSVTINEVPLEDGYLTKQTGDFTIMYSYHDPYSLQ
                     ELHAQSLLHVLPLNKQIDQKEQKRQEESLSEKKQQMAHAVSPTGKVKQTRSLELARIS
                     RKISESNKTIRQEAHLAPQVSGLENEILEPKIKQQSEKEFVAKQSEDQKESDDQKETK
                     KKRLSTIDSTDEHLQPQVPLPSPGTPAPGGGIPSSALGDFMRGMAGTWVYANRNEFDA
                     DDWF"
     gene            2291..2860
                     /locus_tag="ACG842_001230"
     CDS             2291..2860
                     /locus_tag="ACG842_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0247784.1"
                     /translation="MLDKTLPYAEIWMTRPLQQNVPITPLAEGFEFRFYQKEDESAWA
                     EIETSVGEFQTTDEALEYFQQNFAPYPEELERRMFFVETKKGEKVATCTAWRKKSREQ
                     VYPVFHWLAVKPDYQGKGLAKALTARVLQEFPTLHTDGPIYLHTQTWSHQAIALYKKM
                     GFTFIAENLDGTPNPDYEKVMQVLSKLDK"
     gene            complement(3035..3841)
                     /locus_tag="ACG842_001231"
     CDS             complement(3035..3841)
                     /locus_tag="ACG842_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0247785.1"
                     /translation="MNHLKTFFTNSTKTAGRITNGIYILLRIIVVIMLVDQVWNQHWG
                     NTMLLLLTLILFEIPYFLEKILKIQIPNMLEIILLSFIFSSTVLGELSDFYGYFSMWD
                     TFLHAFSGFLAGGVGFSLVYLLNKNTKTMNLTPLLLAVVSFCFSMTVGVMWEFVEFSA
                     DKLLQFDMQKDTIIQQINSVELSKDGNTVYHVPDITRTIIESQNADGKTDKTIIQNGY
                     LDVGIMDTMKDLFVNFIGAVVFSVFGYLYSRNDYQKFTFIKNFIPKKIKR"
     gene            3979..5493
                     /locus_tag="ACG842_001232"
     CDS             3979..5493
                     /locus_tag="ACG842_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavocytochrome c"
                     /protein_id="HFQ0247786.1"
                     /translation="MKKLVAGLLTAVALLAMSACGADQKNTAQTSSSTKESAEVSSGA
                     SEQVYTDPKEMKESYDVVIIGAGGAGMAAAIEAKDAGLNPVILEKMPVAGGNTSKSSS
                     GMNASETKFQKEEGIKDSNDAFYEETLKGGGGTNDKEMLRYFVDHSADAVDWLDQNGI
                     KLDNLTITGGMSEKRTHRPSDGSAIGGYLVEGLLNNVHEREIPIFVNADVTDIKKNGE
                     TVNEVTVHVQGEEPKEVTGKAIVVTTGGFGASKEFIEKYRPDLADYITTNQEGSTGDG
                     ITMIEKLGGQTVDMDKIQIHPTVQQDEGVLIGEVVRGEGAILVDQEGKRFVNEMDTRD
                     KVSAAITALPEKSAYLIFDQGVRDRATAIEFYDQKGYVTTGKTMEELAEKIDLPKETL
                     AKTVDGWNTDVAEKKDAQFNRATAMEHDLSSPNYYAIKIAPGIHYTMGGVKINTNTEV
                     LDKENQPIRGLYAAGEVTGGLHGNNRIGGNSVAEIIIFGRQAGIQAARFASENE"
     gene            complement(5896..6879)
                     /locus_tag="ACG842_001233"
     CDS             complement(5896..6879)
                     /locus_tag="ACG842_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HFQ0247787.1"
                     /translation="MRAISIKHPGSVDVLEQIEVPVPKPKQGELLIKVHTAAVNRTDI
                     MKRESVSLKPPYPILGVEVAGEVVENKSDISTFTPGTKVYGLVNLGGYAEYAVMPADR
                     AILLPDTLDYVSAAGISEVFLTAYQTLYWLGKLQEGETVLIHAGASGVGTAAIQLARQ
                     LSHAKIIVTAGSEEKLAFCKELGADELINYKKQDFQEEVRKITEGRGVDVILDFIGAS
                     YWEKNLASIAVDGRWVLIGMLGGTIVPEIDLGVLIGKRVQLLGTLLTPRSDQYKAELA
                     QTFMQKAGPFLENGKIRPIIDRTFQFSAVKQAHEYMEANKNIGKIILKITE"
     gene            complement(6884..7531)
                     /locus_tag="ACG842_001234"
     CDS             complement(6884..7531)
                     /locus_tag="ACG842_001234"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding oxidoreductase"
                     /protein_id="HFQ0247788.1"
                     /translation="MKIFVVGANGQIGRHLIKDLAPSSHEIFAGVRDVASQTLVKKEN
                     VSYVSFDLTWSVEKMTEAFKGIDVLIFAAGSQGKNLLQVDLDGAIKTVIAAENAHVSR
                     YLMVSAVYADEPAKWPESMTDYYITKHYADEWLKRTNLDFVILQPVTLTNDEEVTSIQ
                     LTKPNEKASKTITRSTVAAVLAALVEETDISRTTLVLSEGSKELNTAFQEWAKEE"
     gene            complement(7616..8248)
                     /locus_tag="ACG842_001235"
     CDS             complement(7616..8248)
                     /locus_tag="ACG842_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFQ0247789.1"
                     /translation="MKNTKNFTLTAMFLAILILLAVTPLGFIPIGPINATTMHIPVII
                     ASIVLGPRLGAFLGGTFGLISMIRSTVIQTPLSFVFSPFIPVIGTEHGSWKALLIAFI
                     PRILIGVVPYFVYKGLRKLMKQKADSVSLFLAGLSGSLTNTILVMNMIYFLFQQDYAK
                     VIGTNLNAVYSAILAVIFTSGIPEGIVAGLATAAVCNVLLRYFKHPSLTN"
     gene            complement(8264..8815)
                     /gene="coaC"
                     /locus_tag="ACG842_001236"
     CDS             complement(8264..8815)
                     /gene="coaC"
                     /locus_tag="ACG842_001236"
                     /EC_number="4.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HFQ0247790.1"
                     /translation="MKKILLGVTGSISAYKSADLTSQFVKLGAQVDVIMSKSSTHFIT
                     PLTLQSLSKRAVHTDVMQESNPQVINHIELAKQADLFLIAPATANIIGKLANGIADDL
                     LSTVAMALPVGTPKLIAPAMNTNMYQNPITQRNLETLRSVGYQEIEPRESLLACGDFG
                     KGALADIQTIIEQVMLTLNERNA"
     gene            complement(8812..9549)
                     /locus_tag="ACG842_001237"
     CDS             complement(8812..9549)
                     /locus_tag="ACG842_001237"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate--cysteine ligase"
                     /protein_id="HFQ0247791.1"
                     /translation="MKILITAGGTTEKIDQVRAITNHSTGRLGQALADYLAANPDTTI
                     DYVTTKQALKPKRHSNITIYTIESALDLFLQLEELTKKEHYDAIIHSMAVSDFTPAFS
                     FSEEQLAKNLPTSSTQEELDNWFAENEQTDTTVSKISSNTEHLVLVLKKTPKIISYLR
                     EWQPKAKIIGFKLLVDVPKESLLAVAKNSLINNKTDFIFANDLTEIHGETHHGYLLSK
                     DGTVEEAQSKSEIAALITEKIRLEESK"
     gene            complement(9546..10535)
                     /locus_tag="ACG842_001238"
     CDS             complement(9546..10535)
                     /locus_tag="ACG842_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HFQ0247792.1"
                     /translation="MNAFRSFQVIKEPVFHTAISDLPFTELAENEVLVKIAYSDVNYK
                     DALAMSESGQVIRTYPMTPGIDLSGTVVQSNNPRFSKEDLVLATGFGLGVTHPGGYSQ
                     YQKVPGDWLVPLPKNMTLRQAMILGTAGFTAMLCVNALIRQGMTSDKKVVVTGASGGV
                     GSTAIALLHKLGFQSIITFSRKEESVTWLKSLGALQVVRPDEFLPETTKALGKQQIDY
                     VIDTVGGEQLSSLLPLISYNGAVALCGNAGGIKLNATVLPFILRNIQLIGIDSVNVPI
                     DQRLSLWQQMADLQIADELVVQEITLDQLPETASKLLAGTHQGRTLVNVGDHK"
     gene            10793..11335
                     /locus_tag="ACG842_001239"
     CDS             10793..11335
                     /locus_tag="ACG842_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HFQ0247793.1"
                     /translation="MEIGEKLRNLRIQKNLTQEELGERTDLSKGYISQLERDLSSPSM
                     ETFFSILEVLGVTPEEFFHQEAANLQVVYSKEDHTVYFDEENGYELEWLVADSNEKEM
                     EPVLLTFEGAGEYKTFEPSLSETFIFVLEGELELSLGETVYVAKAGQSIYYQATKQHQ
                     LRNHLKKRTKVLIVATQSYL"
     gene            11352..12437
                     /locus_tag="ACG842_001240"
     CDS             11352..12437
                     /locus_tag="ACG842_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016185868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0247794.1"
                     /translation="MGKPIISFDQVVKRYDDETVLKKVSFEIEQGKFYTLLGPSGCGK
                     TTILRIIAGFTEATEGDIYFEGKRLNDLPANKRQVNTVFQDYALFPHMNVFDNVAFGL
                     KIKKMSKSDIEKKVKDALRMVQLPGYEKREISEMSGGQRQRVAIARAIVNEPKVLLLD
                     EPLSALDLKLRTDMQYELRDLQQRLGITFIFVTHDQEEALAMSDEIFVMNKGKIVQSG
                     TPVDIYDEPINHFVADFVGESNIVDGVMIEDNLVEFVGKQFECVDGGMRLNEPVEVVL
                     RPEDLTITTPDKGKLVVTVDTQLFRGVHYEIICYDEQQNEWMVHSTKKAKEGSKVGLA
                     FEPEDIHVMRFNESEEEFDARLDSYEE"
     gene            12449..13255
                     /locus_tag="ACG842_001241"
     CDS             12449..13255
                     /locus_tag="ACG842_001241"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0247795.1"
                     /translation="MKTMRRIYSVPYILWLGLFVIAPVLMIIYQSFFDMNGQFTLNNY
                     LSYFTSGTSLSMTINSVWYAFLITFFTLIISYPTAYFLTKLKHKQLWLMLIILPTWVN
                     LLLKAYAFIGIFSVHGSVNHFFDFIGIGPKQILFTDFSFLFVATYIEIPFMIMPIFNA
                     LEEMNPSLISASRDLGANSFETFRRVIFPLSLNGVKSGVQAVFIPSLSLFMLTRLIGG
                     NRVITLGTAIEQHFLVTQNWGMGSTIGVILIIAMFIVMLLTGEKKKGGRS"
     gene            13256..14083
                     /locus_tag="ACG842_001242"
     CDS             13256..14083
                     /locus_tag="ACG842_001242"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0247796.1"
                     /translation="MKKFKWSYLYLGIVFLFLYAPIFYLIFYSFNEGGTMNSFTGFTW
                     ENYEAVFEDTRLIIIVLNTFMLAFLSALIATIIGTFGAMGIYYTKKRRARTALLSMNN
                     ILLVSPDVIIGASFLIFFTLLGFGLGFQSVLLSHIAFSIPIVVLMVLPKLQEMNDSMV
                     DAARDLGANNFQVVKNIILPYLSPGIIAGYFMAFTYSLDDFAVTFFVTGNGFTTLSVE
                     IYSRARQGISLEINALSALVFLFSMILVIGYYFISKENRPKRLKKNRRVREGVARLR"
     gene            14080..15153
                     /locus_tag="ACG842_001243"
     CDS             14080..15153
                     /locus_tag="ACG842_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFQ0247797.1"
                     /translation="MKKLQSLFLGILIIIAVLLLGVRQLEKTSGMSGAKVLTIYNWGD
                     YIDPSLITKFEKEYGYKVNYETFDSNEAMFTKIQQGGTNYDIAIPSEYMIQKMIKEKL
                     VLPLDHSKIKGLNNIDPRFLDLDFDPDNQYSIPYFWGTLGIVYNDKFFDANQIKHWDD
                     LWKPELKDSLMLIDGAREVMGLSLNSLGYSLNSKNMAQLTEAANKLNHLTPNVKAIVA
                     DEIKMYMINEEASVAVTFSGEAADMMWENEHLHYVIPPEGSNLWFDNIVMPKTVKNKE
                     GAYDFINFMLEPENAAQNAEYIGYSTPNEEAKALLPKDISSDEQFYPSDDTISHLEVY
                     KDLGSKYLGIYNDLFLEFKMYRR"
     gene            15581..18277
                     /locus_tag="ACG842_001244"
     CDS             15581..18277
                     /locus_tag="ACG842_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFQ0247798.1"
                     /translation="MEYKYRIDILQPIVYYAFRHIKLEEKMKKSAKDRVFDELIRLTG
                     NMSSVTAEKISKNLGISRQNASHYLTRLVEDKKVEKLPGKPVLWKPLDEYAVVDNTEQ
                     INEAFHSVVGHDGSLREVIQKCIAAVKYPPNGLSVLINGATGVGKSFLATKIFEYAIH
                     EQIIEKDAPFAILNCADYADNPELLSATLFGYKKGAFTGAEKDTDGLLATANNGYLFL
                     DEVHRLSKENQEKLFLFIDTGNYRPVGENVNWHSAKVRFIFATTEKGENYLLDTFDRR
                     IQISVMLPTFEDRPIRERLELIQLFFQNEADVLQKDIIVSREALSLMLSHPFSGNIGK
                     LKNIIKISCADVYSRTQEEPLTIGKNEILIQLNMLESEVESLPIASLLIKKNQKIKTD
                     RIKPFDYDSILEKITQLINEGNFNENFSSIKNEVQHLVHHIDKDSFLSMSRKLMIQMF
                     RKVWVSVLGKTYGLTTSGPVAEIISRSYVISPSYKTDLTGINRTLAANLPRTTYLCNQ
                     FLEHLPPLSEKDENFLRLLIVVTLSDYVDESIELKGLLLAHGDSTASSIQAVVNQLCG
                     NYVFEALDMPVESNVSEIVESTRNFVKRQVNTDSLVLIVDMGSLNQIYTQIKNDLKGE
                     LLLLNNLTTSIALDIGLKMTMNAPFKQIAEDAADKYSINVQYFEGFSQSSNIIISCMS
                     GLGISDKLKDVFRHHLSDESIEVFTKDYRELRELIDQNDETYFDKTKLVITTSDLPRS
                     FSIPNINVYDILDGDGAANLEHILSPEMKPAEFNRLIQELVKFFSLEGIADRLNFLNP
                     TIVMSEVETVISKYENYYHFTLNGRVKLNLYMHTALMMERLFLARTEKSEEPNYDLKP
                     DEDEFYYVSRSIFQSMEMKYNFKVNAYELSLLYELLGPYIQK"
     gene            18342..18842
                     /locus_tag="ACG842_001245"
     CDS             18342..18842
                     /locus_tag="ACG842_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFQ0247799.1"
                     /translation="MIKPMFLKVGTGIAYIKVNRKKGINHMKKILIATHGHLASGFLS
                     SIQLLTGKVQEITVINAYVDECDFEQELNVFVNQVNEQDQIFVFTDLFGGSVNQKITK
                     TFLEKEIAVTILSGFNLPILLEIVLATKELTKEDIQELVNKCQQELKMTILDEVHVVN
                     EEEDFF"
     gene            18854..19348
                     /locus_tag="ACG842_001246"
     CDS             18854..19348
                     /locus_tag="ACG842_001246"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFQ0247800.1"
                     /translation="MIKAVRIDERLIHGQVAMTWTKSLNLTGLVVASDEAATNDIQKM
                     TLKMAAPSNVKVIIKTVDDAIHLLKDPRAKDMRLMVLVPNVKDAVKVAKAFPTEIELM
                     NVGNAGKMTPPSEDKKTLSKEVMLTTSEIQDLKELVELYPDTVFQPTPAMEKRSAISI
                     LSDF"
     gene            19369..20178
                     /locus_tag="ACG842_001247"
     CDS             19369..20178
                     /locus_tag="ACG842_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFQ0247801.1"
                     /translation="MYDAFVVALAVFIGVAGHEMFGMSMLSRPIVVAPLAGALMGDLQ
                     TGLIVGASLEAIFMGVVNIGISSTAEPSLAAGLATAFAIKMGGNVDAIIPIVFPLAVL
                     GLQLMNMIFSFVCGPMAAKFFKFAKSGDQKGIINLHFFMWFVHYALYALIPFFAVLFG
                     ADVVKSVLDNIPEVIMNGLTVAGNLLPAVGMAMLLRMLWSKNIAVWFFFGAVVMAYLE
                     LPLIAIAAVGTIIAIVIAQRDLQLKNISVHAVQSDAAVNATLDQEEEDFFQ"
     gene            20175..20996
                     /locus_tag="ACG842_001248"
     CDS             20175..20996
                     /locus_tag="ACG842_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFQ0247802.1"
                     /translation="MKLADGISKDEKKLVRKMFWRSMTMYASVNPVTMGGGGFAYSMM
                     PFINHFYKSEEDRAAALERHTQYFSTTIPCASFVMGIAASMEKENSEKAGFDVESINA
                     IKTSLMGPLAGIGDSLFWGVWRVVTAGLAINLANAGNILAPIIFLVMFNIPNYLMRYY
                     GGVLGYSLGSKYIEKLYANGLIEILTKAASILGLIMVGAMTISMVKFNTVLSFSMKGE
                     ELMNVQEILDSIFKGIVPLLVTLSCFKLLDKKVGVIKIIFGMVVLGILLSLLGIA"
     gene            21047..23146
                     /locus_tag="ACG842_001249"
     CDS             21047..23146
                     /locus_tag="ACG842_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HFQ0247803.1"
                     /translation="MTINEVIYSPKHPFFNAELNDGNVTTSNEYSYYRNIPDVKGLTF
                     DVSNQKTLANLDVCGTIQTLTFYRENLLTEEKPGVWVNKQLSQTNSLSLKVEVNGIVY
                     HLSESDHRIEVDVIQDCLPRYIHHYSTFKVIVVSFAPILANKRLSMLVQQVYIVNETH
                     ESLQVSVKDVPSYQEKYSDQQNVLISQKGNKDTIAQQEVASFAIAFVDPNAFEEVMTF
                     QESDIEKWLKETLNYFAQLFGQLSMPNDRVVHLYRRALYQSFSSFGMNRQGQVVGSNW
                     GSYPATNRIWNKDMYYASLPFTMFEPELCQKTVLWFDQYGVKFPGSKFTGGINHSLSN
                     SLSSALLASLYFEHTGDTKFFERCPQVLLNASRIIEDILAQRRSGEPMLFPSVWLSDA
                     FALGKYHTGSNLCLWKACIGLAEIFEALEQLSLSKRYKNIACKLKESICKQMTIDGPF
                     GEQFLEGIGDEEKTTYSVKHYQKPILEQGLIFLSDVIVDGKINLLMHDGEESDTTLMP
                     FYQFLDNKDHLYQNTMAFSASSFNPTYSEEIKGITWGLESGATFPGFITVLMSELHNP
                     QAFVTRLEELICLADLDGSWWWWPYRLEAQQGEVVRDFGCGKCGWASGLFVSLMISQY
                     FGLKFKKGVLQIDPVDNLTFSWKNLKFGQAFISIECTQSELSISNLSEKPLKISDTKK
                     ILHVEKGKTIHIQRRKR"
     gene            23143..24261
                     /locus_tag="ACG842_001250"
     CDS             23143..24261
                     /locus_tag="ACG842_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 172 protein"
                     /protein_id="HFQ0247804.1"
                     /translation="MSILKNITQFKKIQSRAITPENPTGEKGKAAMLPSELGPSRKGR
                     GAIPLTSGEETVIADITGTGIIRHMWMTIRDRTEFGSFVLRDLILRIYWDGSDSPAVE
                     SPLGDFFCNGFGERCEVNSLPIVVNPTGGFNSYFEMPFQTSAKITIENLHPKDMSSFF
                     YTINYALVDELEENSLYFHANWHRERITKLQEDYKLIDRLKGQGYYVGTYLALTCLER
                     YWWGEGEFKFYIDGDSEYPTVTSTGSEDYFGGAWAFHEYDEFGRPHAKNYSTAFLGYP
                     YQSNRDATRDRFETGKLNAVHAFGDDAMPRHGLYRWHLQDPICFTEDLSVTLQQIGND
                     DVRLFERQDDVASVAYWYSTEASGNTKRHFTDRERLPR"
     gene            24300..25178
                     /locus_tag="ACG842_001251"
     CDS             24300..25178
                     /locus_tag="ACG842_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFQ0247805.1"
                     /translation="MIYGAIEAGGTKFVCAVGTEELTILERVSFPTTTPVETMKQVID
                     FFKPFKEQLAGIGVGCFGPIDIQCKSTSFGHITSTPKLAWQNFNFVGTLKQYFNIPIE
                     WTTDVNAACYGEYVAGSGKGLAGVAYFTVGTGIGGGALFNGTFVEGFSHPEMGHMLIK
                     RHPKDNFSGNCPFHQDCLEGMAAGPAIEKRLGVKGQNLLADDSFWQIEAFYLAQCAYN
                     TTLMFSPDRIIFGGGVMKQEHMKKKVQDKFVELINGYVEIPPIDSYIITPELGDNAGI
                     IGGLALARKAVRNKQP"
     gene            25324..25521
                     /locus_tag="ACG842_001252"
     CDS             25324..25521
                     /locus_tag="ACG842_001252"
                     /inference="COORDINATES: protein motif:HMM:NF016526.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009372 - quorum sensing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HFQ0247806.1"
                     /translation="MLLRFLFSFCCALYTLRIFTGGVHLNSNILCYFMSVVIVIVAYQ
                     CMFIFRNVDFIILGLMFEFFW"
     gene            25505..25735
                     /locus_tag="ACG842_001253"
     CDS             25505..25735
                     /locus_tag="ACG842_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0247807.1"
                     /translation="MNFFGDRFFQPYKINRNSIIESCDYKKIARKKKKVSIHFKYFIP
                     ISIAKKVEILYLPLTLASLANVVSILIDNKLR"
BASE COUNT         8171 a         4480 c         5440 g         7733 t
ORIGIN      
        1 attattccga ttttcttgaa acaataaaaa atcaaaaaaa atactctcct aatagtccat
       61 cttcttttga ttttatttat ataatgaaat catcttatga aaattactac ttcatgttta
      121 gacaagagta gggaggacta agaataaatg tgttcaaaaa gacgttcaaa acaactaaat
      181 aaacacgctt gtcgtttaga taaggaacgg tcgtgttctg aaaaacacta cgttaagttt
      241 atgatgcgca cgatgctgat cagtttatct gtaggtgtct atatgcatgt acccgttcag
      301 gcaacgacac aaaccatcaa ggtcgtacgt acctctgttt ctgagaccac tgattcccct
      361 atcactatta ctcatacttt cactgatgca aatggaaaac agctacttcc tgcattgact
      421 tcctataata aagtcagtta tcctccaacg ataaaccact actggttaat tcctaacaaa
      481 acattatgga catcggaaac cggaagcagt gtgccgcttt ctatcttttc cgatatgact
      541 gatcctacaa aaggtgatca gttaaaacag atcactgata ttttgaatca tgctttgaca
      601 gatataaagg acggtggaag cgcacaagtt tcttatgtat atgaaaaaga ttattcacag
      661 tttgaaagaa agaaaatcga tgttctggca gggacaaaag tttcgatatc tgatctggta
      721 gaaaggctga agaacgcaga tggaacacaa ggagatatgt ccaaagtaat ttcaaacaat
      781 gggaatatcg tggatacgac taatacagga acatctacta tttccttgtc ctattttgat
      841 ccaattgctt taaaaacaat gactgcatct gctgtgatcc atgttcgtgc tgatcaaaca
      901 gctatcaaag gaaaagatct tcacttaaga acaggagaga gttacgcccc taaagatctt
      961 gttgaatatg taacaaaaag cgatggaact tcaggaaata aagaagaaat caaaatgaac
     1021 gggacagtag atactacaac tccgggagaa tatcccgttg tattaactta tatagactcg
     1081 aaaacatcaa atgtggcaga aacaacagct gtagtttatg tagaagcatc tgctgcttca
     1141 tcgactgtca tcactcattt tatagatagt caaacaaata aagaacttca agcagaaaca
     1201 catatgagta gtgtaaatcg tattcctgat ttgcgcctac ctgctgaatt ttctgatcga
     1261 gtgccaaata tagatttatc gacttatgaa caaaatggga aagtcacttc tatttctgat
     1321 tggatgcaag caaacggatt agacagaaaa agtcactggt cggagatcct tgaagtactt
     1381 cactcagaac tgatgaataa taggaagcaa tcaggagggg aaattacctt tacttatgtt
     1441 tatataccag acaatcgttc attagaaggt catgatctta ttgttcatcc aaacgaaaaa
     1501 ataaacaaag aagagttgat ccaatcagca acaaattcct taggtaaacc cgtaccgtta
     1561 aaggatgtat cggttacaat caatgaagta ccattggagg atggttattt gaccaaacaa
     1621 acaggtgatt ttacgattat gtatagttat catgacccgt attcactcca agaactccat
     1681 gctcagtcac tattacatgt tttaccatta aacaaacaga tagatcaaaa agaacaaaaa
     1741 agacaagaag aaagcttgtc agaaaaaaaa caacaaatgg ctcatgcagt ttctccaaca
     1801 gggaaagtaa aacaaacaag aagcctagag ttagcaagaa tatctagaaa aatctcagaa
     1861 tctaataaaa cgataaggca agaagctcat ttggccccac aagtttctgg attggaaaat
     1921 gaaattttag agcctaaaat aaaacaacaa tcagaaaaag aatttgttgc taaacagtcg
     1981 gaagatcaga aggaatcaga tgaccagaag gaaacgaaga aaaaacgctt gtcaactatt
     2041 gattccaccg atgagcatct gcagccgcaa gtaccacttc ctagtccggg aacacccgca
     2101 cctggtggag gtattccttc ttctgcttta ggagatttca tgcgaggtat ggcaggaaca
     2161 tgggtatacg ctaatcgaaa tgaatttgac gcagatgact ggttctgatt tatatctcta
     2221 tctgttatag tcaaataagt agctgagtca tatgttattt ggcataatgg agacgaggag
     2281 ggatagaaaa atgttagata aaacgttacc ttacgcagaa atctggatga caagaccact
     2341 tcaacaaaat gttccaatca cgcctttagc ggaaggtttt gaatttcgat tttatcaaaa
     2401 agaagatgaa tctgcttggg cggagattga gacttccgtt ggggaatttc aaacgacaga
     2461 tgaagcactt gagtattttc aacaaaactt tgctccttat cctgaagaac ttgaaagaag
     2521 gatgtttttt gtagagacaa aaaaaggaga aaaagtagca acctgtaccg cttggcgaaa
     2581 aaaatcaaga gagcaagtgt atccggtttt tcattggtta gcagtgaaac cagattacca
     2641 aggaaaaggg ctcgcaaaag cattgactgc cagagtcttg caagaatttc ctaccctcca
     2701 tacggacgga ccaatctatc ttcacacaca gacatggagc catcaggcga ttgctcttta
     2761 taaaaaaatg ggttttactt ttattgctga aaatctagac ggcacaccta atccagatta
     2821 tgaaaaagtt atgcaggttt taagtaaatt agataaataa cgaaatggtg aaataagtcc
     2881 tcagtattgg gataaaaaac gaaatagtca ttcctcatat cttacttttt tgagttaata
     2941 ctatcggata actaatggca tgccatacat tcaaaaaaga aggggcagtg acaaaagtat
     3001 tgtcactgcc ccttctcttg ttttttagca tgttttatcg tttgattttt ttaggaataa
     3061 aattcttgat gaacgtaaat ttttggtaat cgtttcgact atagaggtaa ccaaatacac
     3121 tgaaaacaac tgctccaata aaattaacaa ataaatcctt catagtatcc atgattccca
     3181 cgtctaagta accattttgg atgattgttt tatctgtttt accatctgca ttttgacttt
     3241 caatgattgt ccgagtgata tctggaacat gatagacagt gtttccgtct ttacttaact
     3301 ccacactatt gatttgctgg atgatcgtat ctttttgcat atcgaattgc agtaatttat
     3361 ctgctgaaaa ttccacaaac tcccacataa caccaactgt catcgaaaaa cagaaggaaa
     3421 caactgctag taataaagga gtcaagttca tggtttttgt atttttattg agcaaataaa
     3481 caagggaaaa gccgacaccc ccagctaaaa atccgctgaa tgcatgtaga aaagtatccc
     3541 acattgaaaa atagccataa aaatcactta actctcctaa aactgttgaa ctaaaaataa
     3601 aagataacaa aatgatttct agcatatttg gaatctgtat ctttaatatc ttttccagaa
     3661 aataagggat ttcaaataaa attaatgtca gtaataaaag cattgtattt ccccagtgtt
     3721 ggttccagac ctgatccact agcataataa cgacgatgat acgtaataaa atatagattc
     3781 cgtttgtgat tctacctgca gtttttgtac tgttcgtaaa aaaggttttc aaatgattca
     3841 taaaataact ccttcatgta tatccttaat tatactaaga agcgtgtgtt taccaaagct
     3901 atatgactta aagaaaaaca gagttgatat tgtgaaaaaa atcacttaat atagtataag
     3961 gagaaaaggg ggatacaaat gaaaaaatta gttgctggtt tattaacagc cgttgcactt
     4021 cttgctatga gcgcatgcgg cgcagatcag aaaaatacag cgcagacaag ttctagtacg
     4081 aaggaatctg ccgaagtatc atcaggcgct tctgaacaag tatatacaga tccaaaagaa
     4141 atgaaagaat cttatgatgt cgtgattatt ggagctggtg gtgcagggat ggcagcagct
     4201 atcgaagcaa aagatgctgg gttgaatcca gtaattttgg aaaagatgcc tgttgccgga
     4261 ggaaacacat ctaaatcatc aagcggtatg aatgcttcag aaacaaaatt tcaaaaagaa
     4321 gaaggaatca aagactcgaa tgatgccttc tatgaagaaa cgctaaaagg tggtggagga
     4381 acgaatgata aagaaatgct tcgatacttt gtcgatcact ctgctgatgc ggtcgattgg
     4441 ctagatcaaa acggtatcaa actggacaac ttaacgatca ctggagggat gagtgaaaag
     4501 aggacacatc gtccgtctga tggctcggct attggtggat acttagtgga ggggttattg
     4561 aataacgtcc atgagcggga aatccctatt tttgtcaacg cagatgtgac agacattaag
     4621 aaaaatgggg aaacggtcaa tgaagtgact gtacatgttc aaggagaaga accaaaagag
     4681 gtcaccggaa aagcaatcgt agttaccaca ggcggttttg gtgccagcaa agaatttatt
     4741 gaaaaatatc gtcccgattt agctgattat attacgacaa atcaagaagg atcaactgga
     4801 gatggtatca ccatgattga aaaattaggt ggacaaaccg ttgatatgga taaaatacaa
     4861 attcatccaa ctgtgcagca ggatgagggg gtcttgattg gtgaggttgt tcgcggagaa
     4921 ggagcaatct tggtcgatca agaggggaaa agatttgtca atgaaatgga tacaagggat
     4981 aaagtatctg cagcaattac agcattgcct gaaaaatcag cttatttgat ttttgaccaa
     5041 ggcgtgcgtg atcgtgccac agctattgaa ttttatgacc aaaaaggata tgtcacaact
     5101 ggaaagacaa tggaagaatt agcagaaaaa atcgatttac caaaagaaac actagcaaaa
     5161 acagttgatg gatggaatac ggatgttgcg gagaaaaaag atgctcaatt caaccgtgcc
     5221 acagccatgg aacatgactt atcgtctccg aattattatg cgatcaaaat cgcaccagga
     5281 atccattaca cgatgggcgg tgtgaaaatc aatacgaaca ctgaagtatt agataaagaa
     5341 aaccagccaa ttagggggtt atatgctgct ggtgaagtca caggtggctt acatggaaac
     5401 aaccggatcg gtggaaattc agttgcggag atcattattt ttgggcgaca agcgggcata
     5461 caagcagctc gatttgcttc ggaaaatgaa tgaaatagaa cgtaaataaa aagaggttgt
     5521 gaaatcagcg ttttgagcta ataccgaggg tcagctgatt gaacactgtt tagtgaagag
     5581 gttgtgaaaa agctgccctg catcaagtaa taagaacagc caagtaaaaa cagcttctta
     5641 tgtttttaac ttggttggac ttattaccgc agatgcaagc ttttgaacac cgtttattcg
     5701 gaaaaagagc gtgggacaaa agtaaagaac acttttgtct cacgctctaa acacgtataa
     5761 acggcggaag cagaagaaat tccttcggaa ataagctgaa attcacaaaa atttgaaaag
     5821 caattttcgt gaatttcttc ttatttcttg gagttaaacg cttctgtccc agcctctttt
     5881 ttgatcagca tgattttatt cagttatctt caaaatgatt tttccgatgt ttttgtttgc
     5941 ttccatatat tcatgcgctt gtttgactgc gctgaattgg aatgtacggt caatgattgg
     6001 ccgaatcttg ccattttcta gaaaaggccc agctttttgc ataaaagttt gtgcgagctc
     6061 tgctttatac tgatcacttc taggtgtcaa taatgtacca agcagttgga cacgtttccc
     6121 aatcaataca ccaaggtcta tctcaggcac gatggtccca cctagcatac caatcaagac
     6181 ccaacgtcca tcaacggcta tagaagctaa attcttttcc cagtaagaag cgccgatgaa
     6241 atccaaaata acatcgactc cccgaccttc agtgattttt cgtacctctt cttgaaagtc
     6301 ttgtttttta tagttgatca attcatctgc accaagttct ttacaaaagg cgagtttttc
     6361 ttctgatcct gcagtaacga tgattttcgc gtgagaaagc tgtctagcta attggatcgc
     6421 tgctgttccc acaccacttg ctcctgcatg gatcaagaca gtttcgcctt cttgtaattt
     6481 tccaagccag taaagtgttt gataagcagt caaaaaaact tctgagattc cagcagcaga
     6541 aacatagtct agcgtatcag ggagaaggat tgcacgatcg gcaggcatca cagcatattc
     6601 ggcataacca cctagattga ctaatccgta tacttttgtc ccaggagtaa aagtggagat
     6661 atcactttta ttctcaacta cctctccagc tacttcgact cccaaaatag ggtagggagg
     6721 tttcaaagag actgattctc ttttcataat atctgttcga ttgacagctg ctgtatggac
     6781 tttgatcagt aattcgcctt gttttggttt aggaacaggc acttcaattt gttctaatac
     6841 atctacagaa ccaggatgct tgattgaaat ggcacgcatg gatctattcc tcctttgccc
     6901 attcttggaa tgctgtattc aactctttgc taccttcaga aagtactaga gtcgtacgag
     6961 aaatatccgt ttcttccact agagcggcaa gtacagcagc aactgtagag cgagtgatcg
     7021 tttttgatgc cttctcatta ggcttagtta attgaatgga agtaacttct tcatcgttag
     7081 tcaatgtgac tggttgaaga atgacaaaat ctaagttagt tcgtttcagc cattcatctg
     7141 cataatgttt agtaatataa taatctgtca tggactcagg ccattttgct ggttcatctg
     7201 cataaacagc actgaccatc agatagcgag agacatgcgc attttcagct gcgatcactg
     7261 ttttgattgc gccatccaag tctacttgca gaagattctt tccttgagag ccggcagcaa
     7321 aaatcaatac atcgatgcct ttgaatgctt cggtcatttt ttccacggac caagttaaat
     7381 caaaagagac atacgaaaca ttctcttttt ttactaatgt ttgggaagca acatctctca
     7441 cccctgcaaa aatttcatgg gaacttggag ctaaatcttt tatcaggtgt cggccaattt
     7501 gtccgtttgc acctacaaca aaaattttca tttggatcat cctttcgacg tcaaatcttt
     7561 ccattcaata gtaacaccaa aagagaaaaa aatcagagat cggctacgat gactatcagt
     7621 tagtaagaga agggtgcttg aagtatctta acaagacatt gcatactgct gcagtcgcaa
     7681 gacctgcgac gattccttca ggtataccac ttgtaaaaat aaccgctaga atagctgaat
     7741 aaacagcatt taaatttgtt ccgatgactt tggcataatc ttgctggaat aaaaagtaga
     7801 tcatattcat cactaaaatc gtattggtca atgaaccgga aagtccagct aagaataaag
     7861 aaacggaatc tgctttttgt ttcattagtt tgcgcagacc tttataaacg aaataaggaa
     7921 ccaccccaat caagatgcga ggaataaacg cgatcagcaa ggctttccag cttccatgtt
     7981 ctgttccgat gactgggata aacggagaaa agacaaaaga caaaggtgtt tggataactg
     8041 tgctacggat catgctgatc agtccaaatg taccgccaag aaaagcacct agtcttggtc
     8101 ctaaaacgat cgaagcaatg attacaggaa tatgcatggt tgttgcattg attggaccga
     8161 tagggataaa acctaaaggc gtaacagcta ataaaatcaa aatagctaaa aacatcgcag
     8221 ttaatgtaaa gttttttgta tttttcataa gtactccttt tttctaagca tttctttcat
     8281 taagagtcag catgacctgt tcgatgatcg tctgaatatc agctaaggca ccttttccaa
     8341 aatctccaca agctaaaaga gattctcttg gttcaatttc ctgatagccg acagatctca
     8401 atgtttccag attacgctga gtgatcgggt tttgatacat attcgtgttc atggcagggg
     8461 caatcaattt tggtgttccc actggaagcg ccattgcgac tgttgaaaga agatcgtctg
     8521 caatgccgtt tgctagcttg ccgataatgt ttgctgtagc aggcgcgatg agaaacaagt
     8581 cagcttgttt tgctagttcg atatgattga ttacctgcgg atttgattct tgcatgacgt
     8641 ctgtatggac tgcgcgcttc gacagagatt gcaaggttaa tggcgtgata aaatgtgtgc
     8701 tgcttttaga cataatcaca tctacctggg cacctaattt gacgaattga cttgtaagat
     8761 cagcgctttt gtaagcagaa atacttccag taacgcctag taagatcttt ttcattttga
     8821 ttcctccagt ctaattttct cagtaatcaa tgcagcaatc tcagatttac tttgagcttc
     8881 ttccactgtt ccatctttag ataagagata gccgtggtgt gtctcgccat ggatttccgt
     8941 caaatcattt gcgaaaataa aatcagtttt gttatttatc agactatttt ttgcgactgc
     9001 cagaagagat tcctttggta catctactaa tagtttgaaa ccaatgattt ttgcttttgg
     9061 ctgccattca cgtagataag aaatgatttt tggtgttttc ttcaaaacga gcacaagatg
     9121 ctcggtgtta gaagagatct ttgaaacagt ggtatctgtc tgttcatttt ccgcaaacca
     9181 attgtccagt tcttcttgcg ttgaagaagt gggtaggttt tttgccagct gttcttctga
     9241 aaaactaaaa gcaggagtaa aatcactgac tgccatactg tggataatcg catcgtagtg
     9301 ttcttttttc gttagttctt ctaattgtaa aaataagtcc agtgcagatt cgatcgtata
     9361 aatcgttata ttggagtgtc ttttcggttt cagtgcttgt ttcgtagtca cgtaatcgat
     9421 tgtggtatcc ggattggcag ctaaataatc agctaatgct tgccctaatc gtcctgtcga
     9481 atgatttgta atagcacgta cttgatcgat tttttcagtc gttcctccag ctgtgattag
     9541 aatcttcatt tatggtcacc tacatttact agtgttcttc cttggtgtgt tccagcaagt
     9601 agtttagatg ctgtttcagg caattgatcc agagtaatct cttggacgac cagttcatct
     9661 gcaatctgca aatccgccat ctgttgccat aaagaaagac gttgatctat tggtacattg
     9721 acagaatcaa ttccaatcaa ctggatgttt cgcagaataa aaggcaagac agtcgcgttt
     9781 agtttgattc ccccagcatt gccacataaa gctactgcac cgttataaga gatcaatgga
     9841 agaagactgc ttagctgctc gccacctact gtgtcgatga catagtcgat ttgctgtttt
     9901 cctaaagctt ttgttgtctc tggcagaaat tcatcaggtc gtacgacttg caaagcacct
     9961 agacttttta gccatgtgac agactcttct tttctggaaa aggtaatgat tgactggaat
    10021 cctagtttat gtaacagggc tatcgctgta ctgcctacac cgccagaagc accagtaacg
    10081 acaacttttt tatcggaggt cataccttga cggattaaag cgttcacaca caacatagca
    10141 gtaaaccctg ctgtaccaag gatcatcgct tgacgcagcg tcatattttt tggtagagga
    10201 actaaccagt ctcctggtac tttttgatat tggctataac cgcctggatg ggtgacgcca
    10261 agcccaaaac cagtagcaag tacaagatct tcttttgaaa aacgtggatt gttactttgg
    10321 acgacagttc cggataaatc aatccctgga gtcatcggat atgttcgtat cacctgacca
    10381 gactcgctca ttgccaatgc atctttataa ttgacatcag aataggcaat ttttaccaag
    10441 acttcatttt cagccaactc agtaaatggc agatcactga ttgctgtatg gaaaacaggt
    10501 tcctttatta cctgaaaaga tcgaaaagcg ttcattcgtg agacactcct tttaaaattt
    10561 tcttttatca taccataaag cctactcttt tattcttgtt cttccgtaaa tttagacaaa
    10621 aattaggtga aaaataaaga atcaaacaac tagaatttat tccaaaagat aaagaaatat
    10681 tcagcaaaaa aaattttaca aagggttgcc tttttcaata aatttcgtat actaatacga
    10741 gtggtagtaa acaggaagtt tactattaga aaacttcgat tcggaggaaa ttatggaaat
    10801 tggtgagaag ttaagaaatt tgcgcatcca aaagaatttg acacaagaag aactaggcga
    10861 acgcacagat ttgtcaaaag ggtatatttc ccagttggaa cgagatttaa gttcaccatc
    10921 aatggaaaca ttcttttcta ttcttgaagt cttaggtgtg actccagaag aattttttca
    10981 tcaagaagca gcgaatctgc aagtggttta ttcaaaagaa gatcatacgg tttatttcga
    11041 tgaagaaaac gggtatgaat tggaatggct cgtggctgat tcgaatgaaa aagaaatgga
    11101 gcctgtcttg ctgacttttg aaggtgcagg agaatataag acgtttgaac cttcattatc
    11161 tgaaacgttt atttttgtat tagaagggga attagagtta agcttaggag agacggtcta
    11221 tgtggcaaaa gcaggacagt cgatctatta tcaagcgaca aaacaacatc aattaaggaa
    11281 tcatctcaaa aaacgaacaa aagtattgat tgttgcaacg caatcatatt tgtaatatag
    11341 ggggtaaaaa cgtggggaaa ccaatcattt catttgatca agtagtgaaa cgctatgatg
    11401 atgaaacagt attaaaaaaa gtaagttttg agatcgaaca aggaaaattt tatacactgc
    11461 ttggtccttc cggctgtggt aaaacaacga tcctacgtat tatagctgga ttcaccgaag
    11521 ctacagaagg ggacatctat tttgaaggga aacggttgaa tgatttgcca gctaataagc
    11581 gacaagtaaa tacagtcttc caagattatg cactttttcc tcatatgaat gtttttgata
    11641 acgttgcttt tggattgaag atcaagaaaa tgtccaaatc agatatcgaa aagaaggtaa
    11701 aagatgcttt gcgtatggtc cagcttcctg gatacgaaaa acgggagatt agtgaaatgt
    11761 ctggtggtca aagacagcgt gtagcgattg cacgagcaat tgtaaatgaa ccgaaagtat
    11821 tgctattaga tgaaccgtta tcggcgcttg atttgaaact acgtacagat atgcagtatg
    11881 aactgcggga tctgcaacaa cgattaggaa tcacgtttat ttttgttact catgaccaag
    11941 aagaagcgtt ggcaatgagt gatgagattt ttgtcatgaa taaagggaaa atcgtccaaa
    12001 gtggtacacc cgtagatatt tatgatgagc caatcaatca tttcgttgct gattttgttg
    12061 gggagagcaa tatcgtggac ggtgtaatga tcgaagataa tctagttgag tttgttggta
    12121 agcaatttga atgtgtcgat ggcgggatgc gtctaaatga accagttgaa gttgttttac
    12181 gcccagagga tttgacgatc acgacaccag ataaaggaaa actagtcgta acggtcgata
    12241 ctcagctgtt tcgtggtgtc cattatgaaa tcatctgtta tgatgaacag caaaatgaat
    12301 ggatggtcca ttcaacgaaa aaagctaaag aaggaagcaa agtaggattg gcctttgagc
    12361 cagaagatat ccatgtcatg cgtttcaacg agtctgaaga agaattcgat gcacgtcttg
    12421 acagctacga agaatagggg ggacaagaat gaaaacaatg cgcaggatct attccgtccc
    12481 ttatattttg tggctcggtt tattcgtgat cgcgcctgta ttaatgatca tttatcaatc
    12541 tttttttgat atgaatgggc aattcacgct gaataattat ctatcttatt ttacgtcggg
    12601 gacatcgctt tctatgacga tcaattctgt atggtatgca tttttgatca cgtttttcac
    12661 gctgatcatc agttacccga ctgcttattt tttaacaaaa ctgaagcaca agcaattatg
    12721 gctgatgctg attattttgc ctacatgggt gaatctgctg cttaaagctt acgcatttat
    12781 tgggattttc agtgtgcacg gcagcgtgaa tcatttcttt gattttatcg ggatcgggcc
    12841 gaaacagata ttattcacag acttcagttt tctgttcgtg gcaacgtata tcgagattcc
    12901 atttatgatt atgccaattt tcaatgcatt ggaagagatg aatccttctt tgatcagtgc
    12961 tagtagagat ttaggcgcga atagctttga gaccttccga cgagtgattt ttccgttatc
    13021 attaaatggt gtgaaaagtg gtgtccaagc agtatttatt ccttctcttt ctttattcat
    13081 gctgactcgt ctgattggcg gaaatcgggt gatcacttta ggaacggcta tcgaacagca
    13141 tttcttggtt acacaaaact gggggatggg ttctacaatc ggtgtgattt taatcattgc
    13201 tatgttcatc gtgatgctcc tgacaggcga gaagaaaaaa ggaggtcgct cataaatgaa
    13261 aaaatttaaa tggtcgtatc tttatttagg gatcgtcttt ttattcttat atgcaccgat
    13321 cttctatctg attttttatt ccttcaatga aggaggaacg atgaacagct ttacaggatt
    13381 tacttgggaa aattatgaag ctgtttttga agatacacgt ttgatcatta tcgtattgaa
    13441 tacgttcatg ctggctttct tatctgcatt gatcgccacg atcatcggga catttggggc
    13501 tatgggaatc tattacacga aaaaacgacg tgcacggaca gcgttgttga gcatgaataa
    13561 catcttatta gtctctcctg atgttattat cggtgctagt ttcttgattt tctttacact
    13621 actaggattc ggattaggtt ttcagagtgt attgctttcc catattgctt tcagcattcc
    13681 aatcgtcgtc ttgatggtct tgccaaaatt gcaggaaatg aacgattcaa tggtcgatgc
    13741 ggctagagac cttggtgcta ataatttcca agtagtcaaa aatatcattt tgccttatct
    13801 ttctccagga atcatagctg gatattttat ggcatttacc tattctcttg atgattttgc
    13861 ggttacattt ttcgtcacag gaaatggctt tacgacgctc tcagtagaga tctattctcg
    13921 tgcaagacaa ggaatcagtt tagaaatcaa tgcgctaagt gcgttagttt tcttgttctc
    13981 catgatattg gtcattggat actactttat tagtaaagaa aaccgtccaa aacggctgaa
    14041 gaaaaacaga agagtacgcg agggggtggc acggctgcga tgaaaaaatt acaatctctg
    14101 tttttaggta ttctcatcat tattgcggtg ctgctgcttg gtgttcgtca acttgaaaaa
    14161 acaagtggca tgagtggagc aaaggtactt accatctata actggggaga ttatatcgat
    14221 cctagtttga tcacgaaatt tgaaaaagaa tatggataca aagtgaatta tgaaacattt
    14281 gattctaatg aagcgatgtt cacaaaaatc cagcaaggtg ggacaaacta cgacatcgct
    14341 ataccaagtg aatatatgat ccaaaaaatg atcaaagaaa aactagtact tccattagat
    14401 cattcgaaga tcaaaggatt aaataatatt gatccacgat ttttagactt ggattttgat
    14461 ccagataatc agtattcgat tccatatttt tggggaactt tagggatcgt atataacgat
    14521 aaattctttg atgcaaatca aattaagcat tgggatgatt tgtggaagcc ggaattaaaa
    14581 gacagtttga tgctgattga cggtgcacga gaagttatgg ggctttcttt gaatagttta
    14641 ggctattctt tgaatagtaa gaatatggca cagctgacag aagcagcaaa taaattgaat
    14701 catttaacac caaatgtcaa agcaatcgtg gcagatgaga tcaaaatgta tatgatcaat
    14761 gaagaagcct ccgtggcagt gacattttct ggcgaagctg ccgatatgat gtgggaaaat
    14821 gaacatttac attatgtgat cccaccagaa ggatccaatc tttggtttga taatatcgtt
    14881 atgccaaaaa ccgtgaaaaa taaagaaggt gcctatgatt tcatcaactt tatgctagaa
    14941 ccggaaaatg cggcacaaaa tgcagagtat attggttatt ctacaccaaa tgaagaagca
    15001 aaagcgttat tgccaaaaga catttcgtcg gacgaacaat tctatccatc tgatgacacg
    15061 atctcccatc ttgaagtcta caaagatcta ggatcgaaat acctaggcat ctataatgac
    15121 ctattcttag aattcaagat gtataggaga taatggcaaa actcagtaag aatggggact
    15181 tatcaacgtt ctaaagagct aaaaatcgtc ctttttctat ctgagttttc caatcttcta
    15241 ccaaaagcgg atgaaacgcc gctttttttg tgttatttta ttttttctac cacgtttttc
    15301 taccagagaa aagtggacaa aaccattgta aaatagggcc cgagacgggc tagtaaatct
    15361 aagattgaaa aagttacaga aggataaagt aattggaaac tatgcaaata tacttaaccc
    15421 ttctgtaatc tattaataaa actaatatat gaatccagtt caatatttgt agctatgaaa
    15481 aaacataaaa atcatattag ataaaaacta aattggtaga taaattattt tgtcagatat
    15541 ctgaggcaaa tattcgacta tttgtgtttc aaagtaaaaa ttggaataca aatatagaat
    15601 agacatattg cagccgatag tttattatgc ttttagacac attaaactgg aggaaaaaat
    15661 gaaaaagagc gctaaagatc gagtttttga tgagctgata cgactaacag ggaatatgtc
    15721 ttctgtcact gccgagaaaa tttcgaagaa tctaggtatc tctcgtcaga atgccagcca
    15781 ttatttgacg agattagtgg aagataaaaa ggtcgagaaa ttaccgggaa agcctgtttt
    15841 gtggaagcct ttagatgaat atgcggttgt agataatacg gagcaaataa acgaagcctt
    15901 tcattctgta gttggtcatg acggaagtct gagagaggtc attcaaaaat gtatagccgc
    15961 tgtcaaatat ccgcctaacg gactcagtgt attaataaat ggagccacgg gtgttggaaa
    16021 aagtttttta gccacaaaaa tttttgaata cgctatccat gagcaaatta ttgaaaaaga
    16081 tgcaccattt gctatcttaa actgtgccga ttatgcggac aatcccgaac tgctttcggc
    16141 aacactgttt ggatacaaga agggggcctt tacgggggct gaaaaggata ccgatggttt
    16201 attggcaacg gccaacaacg gctatctttt tctcgatgag gtccatcgcc tatcgaaaga
    16261 aaatcaagaa aagctctttc tatttattga tactggaaac tatcgtccag tcggtgaaaa
    16321 tgtaaattgg cattctgcta aggttcgttt tattttcgcc acgacggaaa aaggtgaaaa
    16381 ttatttatta gacacctttg atcggcgtat tcaaattagt gttatgttgc caacttttga
    16441 agatcgcccc attcgagaac gattagagct gattcaatta ttttttcaaa atgaagcgga
    16501 tgttttacaa aaagatatta ttgtttcaag agaagccttg tctttaatgt tatctcatcc
    16561 ctttagtggg aatattggta agctgaagaa tatcattaaa atcagttgtg cggatgtgta
    16621 tagtcgaaca caagaagaac cattaacgat tgggaaaaat gagatcttga ttcagttaaa
    16681 tatgctggaa tccgaagtag aaagcttgcc gattgctagt ttattgataa agaaaaatca
    16741 aaagataaaa acggatcgaa tcaaaccgtt tgattatgat tcgattttgg aaaaaattac
    16801 tcagctgata aatgaaggta atttcaatga aaatttttct tcgataaaga atgaagtaca
    16861 gcatttggtg catcacattg ataaggattc ttttttatca atgagtagaa aattgatgat
    16921 tcagatgttt agaaaggtct gggtatccgt tttaggaaaa acgtacggat tgacgacgag
    16981 tggacctgtt gcagaaatta ttagcagatc ctatgtcatt tccccaagct ataagacaga
    17041 cttaaccggg ataaatcgta cattagctgc aaatctacct cggacgactt acttatgtaa
    17101 tcagttcttg gagcatttac caccgctctc agaaaaggac gagaactttc taagactgct
    17161 tattgtcgtg actttatctg attatgtaga tgaatccatc gagctgaagg gcctgttgct
    17221 tgctcacgga gatagtacgg ctagcagcat tcaagcagta gtgaatcaat tatgcggcaa
    17281 ttatgttttt gaagcgttgg atatgccagt agagagtaat gtgtcagaga ttgtcgaatc
    17341 gacaaggaat ttcgtgaaac ggcaagtgaa tacagatagt ctagtgctga tcgtagatat
    17401 gggctcttta aatcaaattt acacacagat caagaacgat ttaaaagggg agttacttct
    17461 tctgaataat ttgacgactt ccattgcact tgatattggc ttgaagatga cgatgaatgc
    17521 gccatttaaa cagattgcgg aggatgcagc agacaagtat tcaatcaatg ttcaatactt
    17581 tgaaggcttt tctcaaagta gcaacattat catttcctgt atgtcgggtt tggggatttc
    17641 ggacaaattg aaagatgttt ttcgtcatca tttatcggat gaatccatcg aagttttcac
    17701 gaaggactac agagaactac gtgaattaat cgaccaaaat gatgaaactt attttgacaa
    17761 gacaaaattg gtcatcacga cttcggatct tccacgttcc ttttcaattc ctaatattaa
    17821 tgtttatgac atcttagacg gtgatggagc agccaatttg gagcatattt tgagtcctga
    17881 aatgaagccg gcagaattta accgcttgat tcaggagcta gtaaaattct tttcgttgga
    17941 aggaattgcg gaccgtttga actttctgaa cccaacgatc gtgatgagtg aagtggaaac
    18001 agtcatttct aaatatgaaa attactatca tttcacttta aatggccgtg taaaactaaa
    18061 tctctatatg catacggcat tgatgatgga acgtttattc ttggctcgga cagaaaaatc
    18121 tgaagaaccg aattatgatt tgaaaccaga tgaagatgaa ttttattatg tttctagaag
    18181 tatttttcaa tcaatggaga tgaaatataa ttttaaagtc aatgcttatg agctttcgct
    18241 gctctatgaa ttattaggac cgtatattca aaaataaagt gtctaaaaaa gtgtctaatc
    18301 tgtttttttt aggttagaca ctttttttaa tgtgaattaa cgtgattaaa ccaatgtttt
    18361 taaaagttgg cacaggaatt gcttatataa aagtgaacag aaagaaagga attaaccata
    18421 tgaagaaaat tctaatagcg actcacggtc acttagcttc aggctttctt agcagtattc
    18481 aattactgac ggggaaggtt caagagatta cggtgattaa tgcttatgtg gatgagtgtg
    18541 attttgaaca ggagttgaat gtatttgtaa accaggtgaa cgagcaggat cagatttttg
    18601 tttttaccga tttatttggc ggaagtgtca atcagaagat taccaaaacc tttttggaga
    18661 aagagattgc tgtaactatt ttgtctggct ttaatctgcc aattctgctc gaaatagtat
    18721 tggcaaccaa ggaattaaca aaggaggata ttcaggaact ggtaaataaa tgccaacagg
    18781 aattaaagat gaccattctt gatgaggtac atgtagtaaa tgaagaggaa gatttttttt
    18841 aggaggaaga aaaatgatta aagcagtgcg aattgatgaa cgattaattc atggacaagt
    18901 agcgatgact tggacaaaga gtctaaattt aaccgggtta gtagtagcaa gcgatgaggc
    18961 ggctaccaac gatattcaaa agatgacatt gaagatggct gcaccatcga atgtcaaagt
    19021 cattattaaa acagttgatg acgcgattca tttgttaaaa gatccgcggg cgaaggacat
    19081 gcgcttgatg gttttagtcc ctaatgtcaa agatgcggta aaagttgcga aagctttccc
    19141 aacggaaatc gaattaatga acgtggggaa tgctgggaaa atgacaccgc catcggaaga
    19201 taagaaaacc ttatcaaagg aagtgatgtt aacgacgtca gaaattcaag acttaaaaga
    19261 attagttgaa ctttatccag ataccgtttt tcagcctaca ccggcgatgg aaaaacgttc
    19321 agctatctct attttgagtg atttttagaa aaaggaagga gaaaaattat gtatgatgca
    19381 tttgtagtcg ctttggccgt ttttattgga gttgctggac atgaaatgtt cgggatgtcc
    19441 atgctgagtc gtccgattgt tgttgctccg ttagcaggag cgttgatggg ggatttgcag
    19501 acggggttga ttgtcggggc gtcactagaa gccatcttta tgggtgtcgt caatatcgga
    19561 atctctagta cggcggagcc ttctttggct gctgggctgg ctacagcgtt cgcaatcaaa
    19621 atgggaggaa acgtggatgc gattattccc attgtctttc cactagcagt attaggatta
    19681 cagttaatga atatgatttt ttcttttgta tgtggaccga tggctgctaa atttttcaaa
    19741 tttgcaaaat ctggtgatca aaaaggaatc atcaatctgc acttctttat gtggtttgtc
    19801 cactatgcgc tttatgctct tattccattc tttgccgttt tatttggagc agatgttgtc
    19861 aaaagcgtct tggataatat tccagaggtc atcatgaacg gattaacagt cgctggaaat
    19921 ctgctgccgg cagttgggat ggcgatgctg ttacggatgc tttggagtaa aaatattgct
    19981 gtgtggtttt tctttggggc cgttgttatg gcgtatctcg agttaccatt gattgcaatt
    20041 gccgcagttg gaacgattat tgcaattgtt atagcccaaa gagatctaca actaaaaaat
    20101 atatctgttc atgcagttca aagcgatgct gctgtaaatg caacgcttga ccaagaagag
    20161 gaggatttct ttcaatgaaa ttagctgatg gaatttctaa agatgagaaa aaattggttc
    20221 ggaagatgtt ttggcgctcg atgactatgt atgcttcggt gaatccggtg acgatgggcg
    20281 gcggtggttt cgcctattct atgatgccct tcattaatca tttctacaaa agtgaagaag
    20341 atcgagctgc ggcgctggaa cgtcatacac agtatttttc gacgaccatt ccttgcgcgt
    20401 cctttgtcat ggggattgcg gcatcgatgg agaaggaaaa ttctgaaaaa gctggttttg
    20461 acgtggaaag tatcaacgcg atcaaaacga gtttgatggg accattggct ggaattggcg
    20521 attcattgtt ctggggagtt tggcgtgtcg ttaccgcggg actagcgatc aacttagcca
    20581 atgccggaaa tattttggca ccaattatct ttttagttat gttcaatata cctaattact
    20641 tgatgcgata ctatggcggc gttttaggct attcattagg ctctaaatat attgaaaagc
    20701 tgtatgcgaa cgggttaatt gaaattctga caaaagctgc tagtatcttg ggtctgatca
    20761 tggtaggtgc aatgacgatc agtatggtca aattcaatac cgttttgagc ttttcaatga
    20821 agggcgaaga actaatgaat gtccaagaaa tcctcgatag tatttttaaa ggaattgtac
    20881 cgttattggt aacattgagc tgtttcaaat tactagataa aaaagtcggc gtcatcaaaa
    20941 ttatttttgg aatggtcgtc ttgggaattt tattgtcatt acttggaatc gcatagtagg
    21001 aagctgcagc tactaggctg cagttttttt agcaaggagg gaaaagatga ctattaatga
    21061 agtaatttat tcacccaaac acccattttt taatgctgag ttaaacgacg gaaatgtgac
    21121 tacttcaaac gaatacagct actatcggaa tattcctgat gtaaagggat tgaccttcga
    21181 tgtaagtaat caaaagacat tagcaaacct tgatgtttgc gggacgattc aaacgttgac
    21241 cttttatcga gaaaatttac taactgagga aaaaccaggt gtttgggtca ataaacagct
    21301 ctctcaaacc aattcgttat ccttaaaggt tgaagtaaac gggattgtct accatttgtc
    21361 agaatcggat catcgtattg aagtcgatgt gattcaagac tgtttgccta gatatattca
    21421 tcattattcg acgtttaagg tcattgtagt atcctttgct cccatactgg ctaataagcg
    21481 tttaagtatg cttgttcaac aggtttacat tgtgaatgaa acacacgaaa gccttcaagt
    21541 ttcagtgaaa gatgtgcctt cgtatcaaga gaagtattcg gatcagcaaa atgtgttgat
    21601 ttctcaaaaa ggaaataagg acacgatcgc acaacaggaa gtggcaagct tcgccattgc
    21661 ttttgtggac ccaaatgctt ttgaagaagt aatgactttt caggaatctg atatcgaaaa
    21721 atggctgaag gaaacattga actattttgc tcagctattt gggcagctgt caatgccaaa
    21781 cgatagagtt gtccatctct atcgtcgtgc actttatcaa agcttttcta gtttcggtat
    21841 gaatcgacag ggacaggtcg tcggcagcaa ttggggcagc tatcctgcta ctaatcgaat
    21901 ttggaataag gatatgtatt acgcatcatt gccattcacc atgtttgaac cggagttatg
    21961 tcaaaaaacg gttctatggt ttgatcaata tggcgttaag tttcctggtt caaaatttac
    22021 aggaggtatc aatcattctc tgagtaattc gttatccagt gcgttactgg ctagtcttta
    22081 ttttgaacat actggggata caaaattctt tgagcggtgt ccgcaggttc tactgaatgc
    22141 ttcaagaatt attgaggaca tattagctca acgtcggtca ggggaaccaa tgctttttcc
    22201 atctgtttgg ttatcagacg cctttgcact tggaaagtat cataccggca gcaatctttg
    22261 tctttggaag gcctgtattg gacttgcaga aatttttgaa gccctagaac aactgagtct
    22321 tagcaagcga tacaaaaaca ttgcctgtaa actaaaggaa tcaatttgta aacaaatgac
    22381 tatcgatggt ccttttggtg agcaattttt ggagggaatc ggtgatgagg agaaaacaac
    22441 ctattcagtg aagcactatc aaaaaccgat tcttgagcaa gggttaatct ttttaagtga
    22501 tgtgattgtg gatggaaaaa ttaatttatt gatgcatgat ggagaagaat cagacactac
    22561 gttgatgcct ttttatcaat ttttagataa taaagatcat ttatatcaga acaccatggc
    22621 cttttctgct agttctttca atccaaccta tagcgaggaa atcaaaggca tcacttgggg
    22681 attagaatcc ggcgccactt ttcctggctt tatcactgtt ttaatgagcg agctgcacaa
    22741 ccctcaagct ttcgtaaccc gattagaaga actaatttgt ttggcagatt tagacggttc
    22801 gtggtggtgg tggccgtatc gcttagaggc tcaacaagga gaagtcgtgc gagactttgg
    22861 ctgcggcaaa tgcggctggg catcgggtct ttttgtcagc ctaatgatca gtcaatattt
    22921 cggattgaaa ttcaaaaaag gagtattgca aattgatcct gttgataatt taacgttttc
    22981 ttggaagaat ttgaagttcg gtcaagcctt catttcgatt gaatgtacgc aatccgagtt
    23041 gagtatttca aatttgtcag aaaagccatt gaaaataagt gatacgaaaa aaattcttca
    23101 cgtagaaaag ggaaaaacga ttcacattca aaggagaaaa agatgagtat actaaaaaat
    23161 attacccaat ttaaaaaaat ccagagtcga gcaatcactc ccgaaaatcc aacaggcgag
    23221 aaaggtaaag ctgcgatgtt gccaagcgag ctaggaccaa gccgcaaagg tcgtggcgca
    23281 attccgctga cttctggtga agaaactgtg attgcggata ttactggaac gggaataatt
    23341 cgtcatatgt ggatgacgat tcgtgatcga acagaatttg gcagttttgt tttacgtgat
    23401 ttaatcttaa gaatttattg ggatgggtcg gatagccctg ctgttgaaag tcctttaggg
    23461 gatttctttt gtaatgggtt tggagagcgt tgcgaggtga actcgttgcc gatagtggtt
    23521 aatcctacag ggggctttaa ttcctatttc gaaatgcctt tccaaacatc cgctaaaatt
    23581 acgatcgaga atttacatcc gaaggatatg tcctcatttt tttatacgat aaattacgct
    23641 ttggtggacg aattggaaga aaatagccta tattttcacg caaattggca tcgggaacgc
    23701 attaccaaat tacaggagga ttataagtta attgatcgat taaagggtca aggctattac
    23761 gtgggtactt accttgcgct tacctgccta gaacgttatt ggtggggcga aggcgagttt
    23821 aaattctaca ttgatgggga tagtgagtat ccgaccgtaa cctcaacagg ttcagaagat
    23881 tattttggcg gggcgtgggc ctttcatgag tacgatgagt tcggacgtcc acatgcgaaa
    23941 aactattcaa cagccttttt agggtatcct taccaatcaa atagagatgc cacaagagat
    24001 cgttttgaaa cgggaaaatt gaatgccgtt catgcctttg gtgacgacgc aatgccacga
    24061 catggtcttt atcgttggca tcttcaagac cccatctgtt ttaccgaaga tttatcggtc
    24121 actttacaac aaatcggcaa cgatgatgtt cgcttatttg agcggcaaga cgatgttgct
    24181 agtgtcgctt actggtattc aacagaagct tcaggaaata ctaaacgtca tttcactgat
    24241 cgagaacgct tgccgcgtta atgaagtgtt tttcacatat tcgaagggag gaacctatga
    24301 tgatatatgg ggcaattgaa gctggaggga ctaaatttgt ctgtgcagtc ggtactgagg
    24361 aattaacgat tttggaaaga gtcagttttc ccacaacaac tccagttgaa acaatgaagc
    24421 aggtcattga tttttttaaa ccttttaagg aacaattggc aggcatcggg gtcggctgct
    24481 ttggtccaat tgatattcaa tgcaaatcaa cgagtttcgg tcatatcaca tcaacaccaa
    24541 aacttgcttg gcaaaatttc aattttgtag gaacgctaaa gcaatatttc aatattccga
    24601 ttgaatggac aacagatgtc aatgcggctt gctatggtga atatgttgct ggttctggca
    24661 aagggttagc aggtgttgca tacttcactg tgggaacagg tatcggaggt ggtgccttgt
    24721 tcaatgggac tttcgtggaa gggttcagtc atccggaaat ggggcatatg ttgatcaagc
    24781 gacatccaaa ggacaatttt tcaggaaact gtcccttcca ccaagattgt ttagagggga
    24841 tggcagcagg accagcgatt gaaaaacggt tgggtgtaaa aggccagaat ttattggctg
    24901 acgattcgtt ttggcagatt gaagcctttt accttgccca atgtgcatac aatacaacac
    24961 tgatgttttc gccagataga attatttttg gcggtggtgt aatgaaacaa gaacacatga
    25021 agaaaaaagt tcaggataaa tttgttgagc ttataaatgg atatgttgaa ataccaccaa
    25081 ttgatagcta cattattacg cccgaattag gtgataatgc cggaataatc ggcggattag
    25141 cattggccag aaaagcggta agaaataagc aaccataatt aatgaatgtt tcattgatac
    25201 tcatgtcaaa agtatttaac cgagtaatca aatgatcaaa tgaagttgga taaagcgaaa
    25261 atgaattcaa ataaaaaggc tctaagtcaa atcgtgttgt tttcattaaa tcactgaaag
    25321 gaaatgcttc tgcgtttcct tttttctttt tgttgtgcgc tgtatacctt gaggatattc
    25381 actggtggcg tacacttaaa tagtaatata ttgtgttact ttatgtctgt ggtaatagtt
    25441 atagttgctt accaatgtat gttcattttt cgaaatgttg acttcataat ccttggctta
    25501 atgtttgaat ttttttggtg atagattttt tcagccgtat aaaataaatc gtaattctat
    25561 tatagagagt tgtgattata aaaagatagc aagaaaaaaa aagaaagttt ctatacattt
    25621 taaatatttt attcctattt ctattgctaa aaaagtagaa attctatatt tacctttaac
    25681 tctcgcttct cttgcaaatg tagttagcat tttaatcgat aataaactca ggtagttata
    25741 ttaccttgaa atgattgata attttgtcac ttctattttt tttgcaataa attttttatt
    25801 ttagtgaaaa ataagaaact attt
//
LOCUS       DAZKLM010000033        25127 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3764
            SAMEA115706913-rid22582563.denovo.033, whole genome shotgun
            sequence.
ACCESSION   DAZKLM010000033 DAZKLM010000000
VERSION     DAZKLM010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706913
            Sequence Read Archive: ERR13196647
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25127)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25127)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287823.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287823.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,004
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,849
            CDSs (with protein)               :: 2,849
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 41 of 96
            Pseudo Genes (incomplete)         :: 52 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 15 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25127
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706913-rid22582563.denovo.033"
                     /isolate="VRE3764"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..250)
                     /locus_tag="ACG842_001254"
     CDS             complement(<1..250)
                     /locus_tag="ACG842_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4041 domain-containing protein"
                     /protein_id="HFQ0247808.1"
                     /translation="MLLLIGLILLIIGITKLSKANTIKKEAYELEQKNDEKLNKLHDR
                     ERFILSKEETLSDLNKQITMLKNDIILLKKEKEDLNDEI"
     gene            complement(341..793)
                     /locus_tag="ACG842_001255"
     CDS             complement(341..793)
                     /locus_tag="ACG842_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HFQ0247809.1"
                     /translation="MHLNKYWILEEIEKLFVEFGSDAINPYKIIKYSDIDLIETDLGN
                     KTLGQTIHNKRCYVILINQNLSPSMKRFVLWHEIAHVRLHKGICTAAFRANNLSCMIY
                     GIEAEANMFAIEMLTRTIDEQDLSDMSQFQIIEYLGLPHNLEHLVLQH"
     gene            complement(806..1216)
                     /locus_tag="ACG842_001256"
     CDS             complement(806..1216)
                     /locus_tag="ACG842_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFQ0247810.1"
                     /translation="MLLDRIKILLKERSMTLAELERKLNFGNGSMSRWNKSTPSGDKI
                     QKVADYFNVSTDYLLGRTDKRHYYDLTEKDKQDIALQAEKLLEGLNSDAETNYYGEPL
                     DDEDKEKLYDAIQLALALTKVKAKKKFTPKKYRQ"
     gene            1491..1703
                     /locus_tag="ACG842_001257"
     CDS             1491..1703
                     /locus_tag="ACG842_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiprotein-bridging factor 1 family protein"
                     /protein_id="HFQ0247811.1"
                     /translation="MTLKQQVLIALVKKGWSQRELARRMGISITYLRDIFIEKRKPKE
                     RLKQIEELLDIKLEVDSNDQPSEQEA"
     gene            1706..1918
                     /locus_tag="ACG842_001258"
     CDS             1706..1918
                     /locus_tag="ACG842_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0247812.1"
                     /translation="MVQRLSKAEFKEKYKYSESTYQRRMKEFKKSRFSEGYNAVTSKE
                     ITIDVDLYEQFRTWRSKNRFRVRAVC"
     gene            2058..2213
                     /locus_tag="ACG842_001259"
     CDS             2058..2213
                     /locus_tag="ACG842_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0247813.1"
                     /translation="MKEESKIGILNENNYTRLIIETEEGKKVAEVTLTDATPADGYRI
                     RLTPKYD"
     gene            complement(2210..2434)
                     /locus_tag="ACG842_001260"
     CDS             complement(2210..2434)
                     /locus_tag="ACG842_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HFQ0247814.1"
                     /translation="MGKNQHVVPNSNGGWNVKGAGNSKATAHTNTKSEAVKIAREISK
                     NQGSELFIHGRDGKIQSRDSHGNDPHPPKG"
     gene            2512..2700
                     /locus_tag="ACG842_001261"
     CDS             2512..2700
                     /locus_tag="ACG842_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0247815.1"
                     /translation="MAYTLQQEHQILGLIKQRRKQLQDDRAALRKADELSDRQAELIA
                     SELEDLRMLEIKNREIRL"
     gene            2697..2870
                     /locus_tag="ACG842_001262"
     CDS             2697..2870
                     /locus_tag="ACG842_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0247816.1"
                     /translation="MKKTDTLFIGFILGLLVIVAHQSIIGGSLFAALMVLINLLDSKE
                     RSNYGTRRSAKNR"
     gene            2839..2967
                     /locus_tag="ACG842_001263"
     CDS             2839..2967
                     /locus_tag="ACG842_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0247817.1"
                     /translation="MAREEALKIGKVIADNRWGNSRPIILSKQHIEKQKAWQQIKK"
     gene            3026..3361
                     /locus_tag="ACG842_001264"
     CDS             3026..3361
                     /locus_tag="ACG842_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0247818.1"
                     /translation="MNKKIENLIEELKRECQKQGVSIICTAQKEGELKSLVYGETTEI
                     LLCLAMQEEHLDENLPLSAHIMRRIAVDAYEQAKNEEENQPSNHTFVINNKEDLADVM
                     TRILKGEFQ"
     gene            3594..4535
                     /locus_tag="ACG842_001265"
     CDS             3594..4535
                     /locus_tag="ACG842_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lambda-exonuclease family protein"
                     /protein_id="HFQ0247819.1"
                     /translation="MSKSTLEMSHQEWLEDRKKGIGGSDVGTILGLNKWKSPYQLWLE
                     KTGQVVLEESGSEPAYWGNVLEEVVAKEFQERTGKKVRRRNQVFEHPLHPFLRANIDR
                     EVVGENAILECKTANQFLGKEWEGEEVPLSYLCQVQHYMNVLNKDYCYIAVLIGGQKF
                     IWKRIERDQELIDTITEQLVEFWETNVLGGIEPIIDGSQATADFLKEKYADVEDVQTT
                     LPIHFDELVEQKNELKRTKKEIESAIRQVDNEIISELGKREASIGITQKNIISWKFVR
                     TRRMNSKKLAEKYPDVANDEEIYNVTESRRLTEREIK"
     gene            4537..5427
                     /locus_tag="ACG842_001266"
     CDS             4537..5427
                     /locus_tag="ACG842_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecT"
                     /protein_id="HFQ0247820.1"
                     /translation="MATNESLKNQLAAKPQKQVAPGQLGLKALMNTPTMRKKFEEVLH
                     DNANAFMSNVMTLVSNDSYLADSEPMSIMSGALTAATLNLGLDKNLGYAYLVPFNSKN
                     KQTGKWEKKAQFMLGYKGYIQLAQRSGKYKALNVIEVYEGELKSWNRLTEEFEFDPNG
                     RTSDEVIGYVGYFELLNGFKKTVYWTKQEIEAHRIANNKDRDKTKLSGVWASDYNAMA
                     RKTVLRNLLSKWGILSIEMQEATTSDERVQRVQEDGSIIAETEVEEDIPERKEAEVIS
                     EENEDVQTGLFDASNPPLNK"
     gene            5470..6270
                     /locus_tag="ACG842_001267"
     CDS             5470..6270
                     /locus_tag="ACG842_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HFQ0247821.1"
                     /translation="MNTGYVKLHRKVMNSFVWTNPYMYKLWNLCLMKASHENRKILFN
                     GKEIQVNSGEFVTGRDAITSEMNEGVKREHQVNSGSVWRWLKQFEKNGMLNIKSTTKY
                     SVISIKNWSLYQSSEQQMNIKRTTSEQQVHTNKNEKNYKNEKNNKTSSSQPRKKRVYD
                     TDSVYYILAEELFKQICQNQEIKKPNLQSWADDIRKMIEIDKRTENQVRGMIEWSQHN
                     VFWASNILSAKKLREKYDTMAAQANRDYKTKQTKTLEYEKFSTDELPI"
     gene            6285..7136
                     /locus_tag="ACG842_001268"
     CDS             6285..7136
                     /locus_tag="ACG842_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFQ0247822.1"
                     /translation="METVGEIMEKLIQKVLVQRGECPECGQPLYGWRTKNPDGSERCK
                     PTCMQCGYKALRVQEDLQTERIYNESLKARAINFFKGGSVVPNQALFDCTLQNYQIVD
                     QETRQAVEVTKRFVNSVLLGNPSHLVLTGKQGTGKSHLAMAAAWEVLKRSNYDKKILF
                     IGLQEMLDQIKFSYNNPELRKTIEGSLIADIKTADLVIIDDIGSELGKDASDSRAFGI
                     NTLNSFLDARQNLATIITTNLLGEELKKAYGTRTISRMFVNSDGFTMVFSQTADKRIK
                     PVKGAIA"
     gene            7202..7336
                     /locus_tag="ACG842_001269"
     CDS             7202..7336
                     /locus_tag="ACG842_001269"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0247823.1"
                     /translation="MDELSQQESRMVRKISKLIIYRDTERFLTVLGNVYENPELLEVE
                     "
     gene            7337..7798
                     /locus_tag="ACG842_001270"
     CDS             7337..7798
                     /locus_tag="ACG842_001270"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFQ0247824.1"
                     /translation="MPKILDACCGSRLFWFDKNNSDVTFMDCRQQYEELSTGHVINVD
                     PDVVADFRDMPFEDDVFDMVVFDPPHLRHVGENSWLAKKYGKLDELWPEDIRQGFAEC
                     MRVLRPSGTLIFKWNEEQIPLSDVLEAIGEQPLFGNKRSKTHWLVFMKKFS"
     gene            7824..8276
                     /locus_tag="ACG842_001271"
     CDS             7824..8276
                     /locus_tag="ACG842_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010756066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0247825.1"
                     /translation="MKIVLNKCFGGFGLSHEAKMEIFKRKNIEVFPYINNFSYDSDDE
                     YTRYTGQKLGSMDFIYYFKKDPKIDKVTGIYSEIDRLYGIADDSSFSSDSNRGDKDLV
                     AVVEKLGSEASGPYASLKVVDIPDGAEWEISDYDGVETAHYGFQTGSI"
     gene            8382..8576
                     /locus_tag="ACG842_001272"
     CDS             8382..8576
                     /locus_tag="ACG842_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0247826.1"
                     /translation="MDASEVVNKINSLKEVFGDVEVVINAQGYDNEFFKQITDISIQQ
                     GMEDEDGNFIDEVAILVTCE"
     gene            8607..8900
                     /locus_tag="ACG842_001273"
     CDS             8607..8900
                     /locus_tag="ACG842_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0247827.1"
                     /translation="MKLKDGFYSSGHGIGGLMLDMPTKNPKTRKKPKFKVGDMVRCEA
                     EGFIYPFRGYVEHVYNHSAIIRIENTMECDKWLAKSKENLAVVRLVDMEVIKS"
     gene            8831..9370
                     /locus_tag="ACG842_001274"
     CDS             8831..9370
                     /locus_tag="ACG842_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArpU family phage packaging/lysis
                     transcriptional regulator"
                     /protein_id="HFQ0247828.1"
                     /translation="MVSEKQREFSSGEIGGHGSYKILILLFYNYPTTMSVIPQLEGEE
                     AIVNILENIDIKQTRKNARRLLKRYRNLERLVGPVKIDFSVMTVTKNLKFTIDSQNEE
                     IIEAISTRDSVIEALTRLSRIHFQVLYYSYCFPNKMSMYQIGEKLGYSDRTIERMKAV
                     ALVEFAEAYKSGELISRTK"
     gene            9445..9912
                     /locus_tag="ACG842_001275"
     CDS             9445..9912
                     /locus_tag="ACG842_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArpU family transcriptional regulator"
                     /protein_id="HFQ0247829.1"
                     /translation="MVLFDVKKYETPSAKDVDMERTKHNVAVFLSAYLSARCRVGQHR
                     EPKVTASYSLVPPSTADHIFEAERMLIDKEEAQEEFEYLHKLFVRGYSAIQHPHKPDV
                     TERRKKIFYDRYINGLPIYVTAQRNNTSEESVKVESNRIIIQFASSLELVAFK"
     gene            10659..10889
                     /locus_tag="ACG842_001276"
     CDS             10659..10889
                     /locus_tag="ACG842_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0247830.1"
                     /translation="MRNYWYVSLTNEYPRTIDDCSVRVVRSVQIKGKYSIVEMGREAE
                     PCEIDACKLVYCGHGFYDEPNIQNNINKNLRD"
     gene            11048..11881
                     /gene="terS"
                     /locus_tag="ACG842_001277"
     CDS             11048..11881
                     /gene="terS"
                     /locus_tag="ACG842_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit"
                     /protein_id="HFQ0247831.1"
                     /translation="MARQRDPRRDEAKRIWLESNGEKQLKEIASELNVSDSQVRKWKS
                     QDKWSAELISNVTNGKSNVTNQGGAPIGNQNAKGNKGNSRASPPVGNKNALKTGEYET
                     IFFDTLTDNEKDIYSSLNDDPSFALSEEIRLLKIRQLRMMKRIQQAEAGLNDEEVERL
                     QQLRKIKTPIEKNGKKLEIKREVMQDVQISRKKHRKIDDILSIEDSLTRISNQLAKAI
                     KQMNELYMNEYRTDLIKAQTDKIQAETNEIGGNNSGEEIEEWKQAVLNAANKRAVKEN
                     E"
     gene            11874..13289
                     /locus_tag="ACG842_001278"
     CDS             11874..13289
                     /locus_tag="ACG842_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HFQ0247832.1"
                     /translation="MNNEFIPFADIGSAIDYYYDKPVAFCQDILHLNPDEWQENVLND
                     LAEFSKVSVRSGQGVGKTALEAGAILWFLTCRPYAKVIATAPTMKQLYDVLWAEVAKW
                     LNDSLIKNLLKWTKTKIYMVGDSERWFATARTATKPESMQGFHEDHMLIVVDEASGVS
                     DPIMEAILGTLSGFDNKLLMCGNPNNIEGVFYDSHNSDRDKYRVHKVSSYDSKRTNKD
                     NIEMILKKYGKESDVARVRIFGEFPKGALDSFISLETVELATEKQISDSLVNKTKVAH
                     IGVDVARYGDDSTILFPRIATRALEYEKYSKRSTMETTGYVINMAKNLMSQYPSIDKV
                     MIKVDDTGVGGGVTDRLEELIEDKHYPFEVFGVNNGSTSEDDFYDNLGTQLWGNIKEM
                     LEENMTANLNGEQPVIELPSDSSLIKELSTRKFKMTSRSRIRLESKDDMKKRNIGSPD
                     IADALALAFYEPPSHYQFIQF"
     gene            13301..14830
                     /locus_tag="ACG842_001279"
     CDS             13301..14830
                     /locus_tag="ACG842_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFQ0247833.1"
                     /translation="MISTVLSLERYKKLRVKYATQIEDGMFDPNGFIEDMKPFFADRE
                     RKYLAYTSEKNEIDNRPKPNTDIVKVNNKLHAGMYSIVVDQAVNHFTGIPIKWDYDVS
                     EQKRTLIQRLKDKFLKNDIELPTVPEAFNKLTSNLDSMRFAMLDSETATFQGACGVAF
                     RLLEPVEEDDGWKLRASNIEPWRAERYGNAGIYIKEKYDSYQKKFFQEMKVMTRNKIL
                     TYACYGDLNFVTSGTFKKIDETDNPLGTITLAEFKNNTNRYCDFEVAEEIGDAIDRAL
                     SDQQNEIEQFKLAYMLVTGTTMSKGTAKEMMNQLGIINLKAPTAKAEYVTKNLAKDFN
                     EYHMDLLKKQFYTICKAIDFNDEVFKSNSSGEARKWQIISLEAKTNTKEQYFREGLKE
                     CAETIAAFLKFHDKVEIEPEKIIFTFSRSLPTDLSYLAEALPKLAPYVSKRTIQSQIP
                     FVTDVDYENEMMELESGSAYPDSEYNFGGGGNSDDRNEVLDQTSRTGGSSKAQTRKSD
                     T"
     gene            14748..15800
                     /locus_tag="ACG842_001280"
     CDS             14748..15800
                     /locus_tag="ACG842_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
                     /protein_id="HFQ0247834.1"
                     /translation="MTIETKYWTKRRELEDQARLKQENQTLKKLTSVFPEALKEIQAK
                     LLSQADLHNITYPEMMEFYSTSNQKKYREYVEKNYKSLKMYDAKYKEFIDEFFPPFDY
                     AKVNRLLQIRSDVFKILAEYAMDADVNQYFSDRLEEILQRTYSSNANVFVQLLNVDIP
                     NYLPENEIKHYLNYPWSGKTFSRRLWGNISSLEQKLSNAIVKSVASGEGVIHALNTMR
                     LDSEICDMFKLEESKYNKAIENLVRTEYAKFAQDGIEKSYLETGIEEYNVLTAKDERV
                     CRICGGKASKNPYKLKDAVIGENRAPFHSRCRCTDVPNLPKLGKDIDEEYDRLFGDLL
                     DEFAHDSFGINLKRRK"
     gene            15802..16119
                     /locus_tag="ACG842_001281"
     CDS             15802..16119
                     /locus_tag="ACG842_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0247835.1"
                     /translation="MKDFFEAVLTINVNADIAEAYKTAIESENHPNGLRDHWNGNYAY
                     VVIGDQTVNYQDNTPVDKNTVNLTIQLLSHSLPNLKETVDWYEKMGCIVVRTDYKEGK
                     SSN"
     gene            16246..16869
                     /locus_tag="ACG842_001282"
     CDS             16246..16869
                     /locus_tag="ACG842_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4355 domain-containing protein"
                     /protein_id="HFQ0247836.1"
                     /translation="MSMKHKSLMPMNLQYFAEGDDQKFSFDDFKSFVESNEEAQKFIQ
                     SQSQSVADKQLEAWKQNNLDKIKQDTIKEYEESKKNKSPEQIQLEKLQAEFEAEKALR
                     VTSDNKAFVAEQIAGLELDGELKESISQFMLNNLVSSDTDFTKNAVEGFTSVLNAINE
                     KHADALKELQMKSAFGGTQQSNNQVQQNNETLTNPEEQLGQILQQFN"
     gene            16884..17774
                     /locus_tag="ACG842_001283"
     CDS             16884..17774
                     /locus_tag="ACG842_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5309 family protein"
                     /protein_id="HFQ0247837.1"
                     /translation="MKKTSLNNLEYLDISPAINAMQVPNTPFLSYLLGAGKTEPANST
                     EIKWREYDINNDDSSEKLEGGEYPDAESGRTWFNNYTEIFRKSTSVSGTLDAINVNGV
                     GNELTNQVALRGMEMKIDLNRKLITGVKADENGSKGRRMNGILNLINSANKAETATAG
                     AVTRKDIDTLFKLMYEKGYMGEKLCLISPDMQELMTDELDGKSTKIVQFGERVTFGLQ
                     IGNIVSNYGTGIALLEPSLPKGTIAAIDTNYVKLRPLREWRAEELAKTTDSRRIGLVG
                     EYSLEYNASNSGAILNLKSE"
     gene            17797..18012
                     /locus_tag="ACG842_001284"
     CDS             17797..18012
                     /locus_tag="ACG842_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0247838.1"
                     /translation="MIGGITVVKKDETKKDEVVKYRVGKTKNFVGFVHPKTRRFITAD
                     SNNEFIISIDDKEAIAILEDAIDVNEI"
     gene            18024..18365
                     /locus_tag="ACG842_001285"
     CDS             18024..18365
                     /locus_tag="ACG842_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0247839.1"
                     /translation="MDESLKTEIIESTKEDFPDLSEERITNLLEIILLEIESYNTCKN
                     DISWEKLKSVISEVLYKIIKNESEKTVSSVRRGDTTISYASTTNDVSELLLGYGDLIR
                     RVIGCGGLEFF"
     gene            18365..18736
                     /locus_tag="ACG842_001286"
     CDS             18365..18736
                     /locus_tag="ACG842_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0247840.1"
                     /translation="MNEADILETTYEDSCIIERLTDIEDSNTNITIQDYKKVYDNPIS
                     CALSQGQIDGLAVIEDGEMVNVSTDTYKLFVHPKIKLKKGDRITITQKASGLIFSLFA
                     TKPFYYPSHCEVNLIGSEKNG"
     gene            18729..19124
                     /locus_tag="ACG842_001287"
     CDS             18729..19124
                     /locus_tag="ACG842_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HFQ0247841.1"
                     /translation="MGNLKYESNAEKIIENFKNMTVIAQKEGISFVNDSMNKVVSLAK
                     PLTPVKSGNLRRGYRVVKARKLSSGRIVGEVINNEHYFKYVEEGHRTKNGGFVKGKFM
                     LTRATNLANMSYIPRRFKQMAIKIVKKGK"
     gene            19126..19503
                     /locus_tag="ACG842_001288"
     CDS             19126..19503
                     /locus_tag="ACG842_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6838 family protein"
                     /protein_id="HFQ0247842.1"
                     /translation="MKDEIIAAISSKLKEIYPDGTIYLDSVMQSTKDFYFVLSVMESG
                     TENVGIDIQNVSFLIDIALIDNNPNRNLINELVSRCGTFFNTITIDEQILFPKAYLPD
                     EADGVQHIRFTLEFPQYIEWSER"
     gene            19504..20112
                     /locus_tag="ACG842_001289"
     CDS             19504..20112
                     /locus_tag="ACG842_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="major tail protein"
                     /protein_id="HFQ0247843.1"
                     /translation="MGEKRSKTGIISVEKPTWFPLEDETGNFPVYGTALPMGTAVSIK
                     PTANYETTQDYGDSVVQDQFTAFGGAEVELEANGYTHKVLSTITGGKIVKGGALRSGE
                     DIAQDGAFAYRRKKSNGKYRYTVFYKGQFALDSDETSTIEGSKVSFTHPTWKGSFVDV
                     PGLGYMYSVDEDDEGVDKAMIGNWFTKVAIPIEEAELSGGTE"
     gene            20112..20453
                     /locus_tag="ACG842_001290"
     CDS             20112..20453
                     /locus_tag="ACG842_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0247844.1"
                     /translation="MSKYQTTIKLTKKDEEGKYEQVQFKSAEFLPGTVVEDAAGVMEE
                     MQTATDKQSVKKALSRAYSFIADTLFEGQFTGEDYCKGIDAREIASLTGKLLKSVTAG
                     FDETYTETKKK"
     gene            20695..23484
                     /locus_tag="ACG842_001291"
     CDS             20695..23484
                     /locus_tag="ACG842_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HFQ0247845.1"
                     /translation="MILDESGFTAGMNNAVKKLNSFDETIERKSRNSGSSLGSIWKIF
                     AGSFLASGVTRIVGAGFDLIKGSISGAVDRVDTMNNALRNFQNMGFSNSEIMKNIGKN
                     GLLSQGIQGLPTALNDAISHVQLLASSTGDLTRSTQIFKALNDGILGFGGSTDQVNEA
                     VIQLSQSFSNGKVDAQTWNSMINAQLGPTLSAIAKKMGITMGDLKEGLSQGKISVEEF
                     QNQLIEMDTKGGGGLKSLSQIAKDSTKGIKTSIQNAKTAVTRGVGEVIEGLNKALVDS
                     DLGGFKGIIDKVGSSMESFLKVIAANIPTAVSFLSNLFNEIQKFGSALKFMMPFLVPA
                     ATAFGAFMFQLKGIPAIIKSFNNFKNAIIGVGNSLKIMGAIAAANPFVLIIGAVVGAI
                     AVFGYFMATNEELRNKVISIWNDVKDSVIGVLKNIKDWGIDTWNSAKEMASNAVEGVK
                     DAWSGTKEWFSNTWKGIKDGATGLFDKTVETSRNAVDSVKNAWSNMKKWFSDTWQSIK
                     DSAREKWNGIKGSIMSVAGPLITGIKNAFLHVTFYLETLWNNLVEIGKNVFEILKNVI
                     LAPVLFITSMISGGWEETKNNMIAVWNNIKESAINIWESIKNIFVSYFTNIYFAALNI
                     WTGFKLTLINIWNEVVNQAKSIWLNIKYFFINLWIDIKYFAIQKWIELKFSIIQTWID
                     LKYNAITTWNNIKQFFKDTWKNIKDTAYNTWISIKNTMINTWNNIKDSFWNIVTGIVN
                     SAENAWTNLKNGVSNAINRVKEIFNSLREVNLFEIGKNIIDGLINGVKEKWNALKDTI
                     KGIAGNIKNWIKGALDIHSPSRWMRDMVGKNIVQGIIVGIDKEQSKLDQTMTNLVKTP
                     SVQPVVNGISHLPITQTKQNISASETKEIHLHLNVYGDLPDSMVRKITTKIKTELTRQ
                     MKRDAGAVGGTLYAT"
     gene            23474..24214
                     /locus_tag="ACG842_001292"
     CDS             23474..24214
                     /locus_tag="ACG842_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFQ0247846.1"
                     /translation="MQLKRGQFFINQHYSSEFNVYIQNRPASVSASRVIELREREGND
                     SIIIDKGYYKNVTRKIECYYKAPSIDMVQEWEDRITEWLDMSSYSDFILYYDEQYIYQ
                     AVVIEGPEFKGTRKTGNIVPFEFTVSIRPFKENYNGRFTIRQTETFEIYNPEKYSSKP
                     LIKLSGSGDASFYINKDKYDLKSLDRELFIDSKLEEAYRKLDGNLEHQDQVTLFLDFP
                     FLYPGKNEIKWTKNIHSFEIMPRWWRKV"
     gene            24211..>25127
                     /locus_tag="ACG842_001293"
     CDS             24211..>25127
                     /locus_tag="ACG842_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail spike protein"
                     /protein_id="HFQ0247847.1"
                     /translation="MKPRIYSPTETDFSTNGLGILKDCTRCGIYEVANGKYELELDYP
                     LGTRFDEYFENDYQIKAKPNDQEEYHIFFIDDKDIDTFLNTVTIYAQSRTNRLGRRVV
                     TLVEVDSKTGQEAISIIETKMDKKSDIRLYSDITAVSSTIFEARNVLNCIAGEQGSLL
                     QYWGGEIKREPFKLSLLKRRGRNNVGTIRYGKDMSGLKVKLDWTGIKTRIIPYADPQS
                     EVGTTNRIYGSPVDSAYINNYPDVYTEHVQFTEEQGVKDIKSLNKIAKNYFKTINPGC
                     DKPKVSITVEFDKLTDSEEAKEFAKIRNYG"
BASE COUNT         9052 a         3619 c         5093 g         7363 t
ORIGIN      
        1 ttatttcatc atttaaatct tctttctctt tttttaataa aataatatca ttttttaaca
       61 tagtaatttg tttatttaaa tctgatagtg tttcttcctt acttaatata aatctttctc
      121 tatcatgtaa tttatttagt ttttcatcat ttttttgttc taattcataa gcttcttttt
      181 ttattgtgtt agctttcgac agtttagtga ttcctataat taataaaata agtccaatca
      241 ataataacat tcaaaactcc tcatttcttt gatataatta ttgtagagtt tactcagaaa
      301 tgaggaaaga gtccatgtga aagcatgggc ttttttattt ttaatgttgt aatactaaat
      361 gttcaagatt atgaggaagc cctaaatatt caataatttg gaactgactc atatcagata
      421 aatcttgttc atctattgtt ctagttaaca tttcaattgc aaacatatta gcttcagctt
      481 ctataccata aatcatacaa ctaaggttgt ttgctctaaa agctgctgtg caaatacctt
      541 tatggagtct aacgtgggct atttcatgcc ataatacaaa tcttttcata gaaggtgata
      601 ggttttgatt aattaatatc acataacatc ttttattgtg tatcgtttgt cctaatgttt
      661 tattccctaa atcagtttca attaaatcta tatcagaata tttgattatt ttatatggat
      721 taatcgcatc agatccaaac tctacaaata atttttctat ttcttctaat atccagtatt
      781 tgtttaaatg cataccaaag cccacctatt gacgatattt tttaggagtg aattttttct
      841 ttgctttaac tttggttaag gcaagtgcaa gttgaatggc atcgtataat ttttccttat
      901 cttcatcgtc taaaggttca ccataataat ttgtttcagc atctgaattc aagccttcaa
      961 gtaatttttc agcttgtaaa gcaatatctt gtttatcttt ttctgttaaa tcataataat
     1021 ggcgtttatc agtacgtcct aaaagataat cagttgatac attaaagtaa tctgcaactt
     1081 tttgtatttt atctccagat ggagtacttt tattccatct agacatgctt ccatttccga
     1141 aatttaactt tctttcaagt tctgccaacg tcattgaacg ttcttttaat aagattttta
     1201 tacggtctaa tagcatagga tatgcattcc tttcaaaata ccgacaaaaa aagtattttt
     1261 ctgcaaatta tctatttaca aatagagaat ttgcagttat actagttttg taagcaagat
     1321 atttacggca tacaaagact aaaagcaata aactaatagt ctccccagac atcagtaagc
     1381 tcgctttttg tgcgctgtat tttgtatgct tacataatag catatttgca gtttttgtca
     1441 aacttattag acaaaaatat tagaaaatat tcgataaggg ggtatgaagc atgactttaa
     1501 aacagcaagt attaattgct ttagtaaaga aaggttggag tcaaagagag ttagctcgac
     1561 gaatgggtat atcaattact tatttacgag atatcttcat cgaaaaaaga aaacctaagg
     1621 aacgtttaaa acaaatcgaa gaacttttgg atatcaagtt agaagtagat tctaatgatc
     1681 agccatcaga acaggaggcg taaaaatggt acaacgtttg agcaaagcgg agtttaaaga
     1741 gaaatataag tattctgaaa gtacttatca aagaagaatg aaggaattta aaaaatcacg
     1801 ttttagtgag gggtataacg cagttacttc caaagaaata acaattgatg tcgatttata
     1861 tgaacagttt agaacatggc gttctaaaaa tcgatttaga gttagagctg tttgttaata
     1921 gctaaaaaat ctaaattcac cacttctgaa aagaggagtt gctgctctcc tttcaagttt
     1981 gccaaagtcc acgttttttc attttggcaa ctcctcttct tggaggtggt gacggcgaga
     2041 gacgaaggag ggatgacatg aaggaagaat caaaaatagg gattttgaat gagaacaatt
     2101 acacaagatt aattattgaa acagaagaag ggaaaaaagt agcagaagtt acattgaccg
     2161 atgcaactcc tgctgacggt tatcgtattc gattaactcc taaatatgat taaccttttg
     2221 gtgggtgtgg atcgtttccg tgactatcac ggctttgaat ttttccatct ctgccatgaa
     2281 taaatagctc agatccttga tttttagaaa tctctctagc gatttttact gcttctgatt
     2341 ttgtattagt atgtgcagtc gctttagaat ttccagcacc tttgacattc caaccaccat
     2401 tggaattagg tacaacatgt tgattttttc ccatagtttt cacctccatt tctatagaaa
     2461 tattttatca aatagaaatg gagaaattca ataaacgaaa ggagaagtga tatggcatat
     2521 accttgcaac aagaacatca aattctcggt ttgattaaac aacgcaggaa acaattacaa
     2581 gatgaccgtg cagcgcttag aaaagccgat gagctatcag ataggcaagc tgaactaatt
     2641 gcttctgaac ttgaggattt gagaatgcta gaaataaaaa atagggagat tagattatga
     2701 agaagacaga cacacttttt ataggattca ttttggggtt attagtgatt gtagcgcacc
     2761 aaagtattat cgggggaagc ttgttcgcag cattgatggt tttaatcaat ctgcttgatt
     2821 caaaagaaag gagcaactat ggcacgagaa gaagcgctaa aaatcggtaa agtgattgct
     2881 gataatcggt ggggaaatag ccgccctatt attttaagca agcaacatat cgaaaagcaa
     2941 aaagcatggc aacaaataaa aaagtgactc tgccagccag caaagagtca catacaaatt
     3001 tataaaacta ggagaattat atcacatgaa taagaaaatt gaaaatctta tcgaagaatt
     3061 gaaacgtgaa tgccaaaagc aaggtgtttc tattatttgt acagcccaaa aagaagggga
     3121 acttaaaagt ctcgtttatg gtgagacaac agaaatttta ctttgtcttg ctatgcaaga
     3181 agaacattta gatgaaaatc tcccattgtc agcacatatt atgcgtagaa ttgctgtgga
     3241 tgcttacgaa caagcgaaaa atgaagaaga aaatcaacct tcaaatcata cttttgttat
     3301 taataacaag gaagatttag ctgatgtgat gactcgtatt ctcaaggggg aatttcaatg
     3361 atgaatgaaa aaacaattga tctactcatt attttatttt tcacgttagt gattgtagcg
     3421 aaaagttatg taatctttgg cttgttattc gcaattacca tgcttgtgct ttgcgtgtta
     3481 gcaagcagaa aagaggttaa acatgaacga gttagcaaat cttgacaact atttaactga
     3541 tcctgattat gctaaaccac catatgaagc accaattgat gaggaggatg aagatgagta
     3601 aatctacctt agaaatgagc catcaagaat ggcttgaaga ccgtaagaaa ggcatcggag
     3661 gttcggatgt tggaacaatt ttaggattga ataaatggaa atctccttat caactatggc
     3721 tagaaaaaac aggacaagta gtacttgaag aatcaggaag tgagcccgct tattggggta
     3781 atgttttaga agaagtggtt gctaaagagt ttcaggaacg tacaggcaaa aaagtacgca
     3841 gaagaaacca agtatttgaa catccattac atccattttt aagagcaaat attgatcggg
     3901 aagtagtggg agaaaatgcc attctggaat gcaaaacagc caatcaattt ctcggtaaag
     3961 aatgggaagg cgaagaggta ccgcttagct atctctgtca agttcaacat tacatgaacg
     4021 ttctaaacaa agactattgt tacatcgctg tcttaatcgg tggtcaaaaa tttatctgga
     4081 agcggattga acgagatcaa gagctgatcg atacaatcac tgaacaatta gtagagtttt
     4141 gggaaacaaa cgttcttgga ggtatcgagc caattattga tggtagtcaa gcgacagctg
     4201 atttcttgaa agagaagtat gcagatgttg aagacgttca aacgacgtta ccaattcatt
     4261 tcgatgaact agttgaacag aaaaacgaac tcaaacggac taagaaagaa attgaatcag
     4321 ctatccgtca agtagacaac gagattatca gcgagctagg taaacgtgaa gccagtatcg
     4381 gtatcactca aaaaaacatc atcagttgga aatttgtccg tacgagacgt atgaactcga
     4441 agaaactagc agagaaatat ccagatgtcg caaatgatga agagatttat aacgttactg
     4501 aatcaagaag gctaaccgaa agggagatca aataatatgg caacaaatga atcgttaaaa
     4561 aatcaattgg cagcaaagcc acagaaacaa gttgcaccag gtcaattagg acttaaagct
     4621 ttgatgaata caccaacaat gagaaagaag ttcgaagagg tgcttcatga caatgctaat
     4681 gcttttatgt cgaatgttat gactcttgta tctaatgaca gttatcttgc agatagtgaa
     4741 ccgatgtcta tcatgagtgg tgcgttaact gctgcaacat taaatcttgg gctagataag
     4801 aatttaggtt atgcatattt agttccattc aatagtaaaa acaagcaaac aggaaaatgg
     4861 gaaaagaaag ctcaattcat gcttggctat aaaggatata tccaattagc ccaacgatca
     4921 ggtaaataca aagcattaaa tgtgatcgaa gtttacgaag gagaactaaa aagctggaac
     4981 cgactgacag aagagtttga gtttgatcca aatggtagaa cgtctgatga agtcattgga
     5041 tatgttggct atttcgagtt actgaatgga ttcaaaaaaa ctgtctattg gaccaaacaa
     5101 gaaatcgaag ctcatcggat tgctaacaat aaagatcgcg ataagacaaa gttaagtggt
     5161 gtgtgggcat ctgattacaa tgcaatggca cgaaaaactg ttttgagaaa tcttctttct
     5221 aaatggggaa tcttgtccat cgaaatgcaa gaagccacca catcggatga gagagtccaa
     5281 agggttcaag aagacggcag cattattgct gaaacagaag ttgaagaaga tattcctgaa
     5341 agaaaagaag cagaggttat atctgaagaa aatgaagatg tacaaactgg attatttgat
     5401 gcatctaatc cgccgttaaa caaataatga gggagctttc tcccttgctt ttctagagga
     5461 gaaatacgaa tgaacacagg atatgtcaaa ttacatcgaa aagtgatgaa ttcattcgtt
     5521 tggaccaatc cttatatgta taaattatgg aacttgtgtt tgatgaaagc gagtcatgag
     5581 aatcgcaaga tactttttaa tggaaaagag atacaagtga acagcggaga attcgtcaca
     5641 gggcgcgatg ctatcacatc tgagatgaac gaaggcgtga aacgtgaaca tcaagtgaac
     5701 agcggttctg tatggagatg gctaaaacaa tttgaaaaaa acggaatgtt gaacatcaaa
     5761 tcaactacga aatacagcgt tatatcaata aaaaactggt ctttgtacca aagcagtgaa
     5821 caacaaatga acatcaaacg cacaacaagt gaacaacaag tgcacacaaa caagaatgaa
     5881 aagaattata agaatgaaaa gaataataaa acatcatcgt cgcaaccacg caaaaagcgt
     5941 gtttacgaca ccgactcagt ttactacatt ctcgcggagg agttattcaa acagatttgt
     6001 cagaatcagg aaatcaaaaa gccgaatctg caaagttggg cagatgacat tcggaaaatg
     6061 atcgagatcg acaagcggac cgaaaaccag gtacggggaa tgattgaatg gagtcagcac
     6121 aatgtgtttt gggcatcgaa tattttatct gctaaaaaat tgcgagaaaa atacgacaca
     6181 atggcagcgc aagcaaatcg tgattataaa acaaaacaaa ctaaaacgct tgaatacgag
     6241 aaatttagca cagatgagtt gcctatttga gaggaggcgt aggcatggaa actgttgggg
     6301 aaatcatgga aaagctgata cagaaagtgc ttgtccaacg tggcgaatgt cctgaatgcg
     6361 gacagccttt gtatggatgg cgtacgaaga accctgatgg ctcagaacgt tgtaagccaa
     6421 cctgtatgca atgtgggtat aaagcattgc gagttcagga agacttacaa acagaacgaa
     6481 tttacaacga gagcctgaaa gcaagagcaa tcaatttttt caaaggtggt tctgttgtgc
     6541 ctaatcaagc gttgtttgat tgcacattgc agaattatca aattgtcgat caagaaacaa
     6601 gacaagcggt tgaagtaacc aaacgctttg ttaattcagt cttgttagga aatccaagtc
     6661 accttgtttt aactggaaaa caaggaacag gtaaaagtca cctagcgatg gcagcggctt
     6721 gggaagtctt gaagcgatca aactacgata aaaaaatctt atttatcggg ttacaggaaa
     6781 tgctggatca aatcaaattt tcgtacaaca atcctgaact cagaaaaacg attgagggat
     6841 cgttgattgc agatatcaaa acagcggatt tagtcatcat tgatgatatc ggttcagaac
     6901 taggaaaaga tgcatcagat agtcgagcgt ttggcataaa cacgctaaat tcgttcttgg
     6961 atgcacgaca gaacctagca acgattatca caacgaactt gcttggtgaa gaactgaaaa
     7021 aagcttacgg tacgagaaca atatcaagaa tgtttgtcaa ctctgatgga tttacgatgg
     7081 tattttctca aacagcagac aagcgcataa aaccagtcaa aggagcgatc gcatgaattt
     7141 gactaaacaa gaaaaaagaa tctgtcgtca gataatgttg caaattatag gtgatcgtga
     7201 catggacgaa ttatctcaac aagagagtcg aatggttcgg aagataagta agctaattat
     7261 ttatcgggat acagaaagat ttctcactgt tttaggtaac gtctacgaga atccagagtt
     7321 attggaggta gagtaattgc caaaaatatt agatgcatgt tgcggtagta gattgttctg
     7381 gtttgataag aacaattctg acgtaacatt tatggattgt cgtcagcagt atgaagaatt
     7441 atcaacaggt catgtgatca atgttgatcc agatgtggtc gcagactttc gcgatatgcc
     7501 ttttgaagat gatgtgttcg atatggttgt atttgacccg ccacacttaa gacatgtcgg
     7561 cgaaaactca tggcttgcca aaaaatatgg aaaattagac gaactttggc cagaagacat
     7621 caggcaaggt tttgcagagt gtatgcgagt tttacgacct tcaggaacat taatttttaa
     7681 gtggaacgag gaacagatac cactttctga tgtgttagag gctattggag aacagccatt
     7741 gtttggtaat aaacgaagca aaacacattg gcttgtgttt atgaaaaaat tcagctaacg
     7801 gaggaaacaa aggaggaaaa gtaatgaaga ttgtattaaa taaatgtttt ggtggctttg
     7861 ggttgagcca tgaagcaaaa atggaaattt ttaaaagaaa aaatattgaa gtattcccat
     7921 acatcaataa ctttagctat gactcagatg atgaatacac gagatacaca ggccaaaagc
     7981 taggttcgat ggattttatc tactatttca aaaaagaccc gaaaatcgac aaagttacag
     8041 gaatttatag cgaaattgac cgactgtacg gaattgctga tgattccagt ttttcaagtg
     8101 attcaaacag aggtgataag gacctcgttg ctgttgtcga aaaactagga agtgaggcca
     8161 gtggaccata tgcaagtctt aaagtcgttg atatacccga tggggctgag tgggaaataa
     8221 gtgattatga cggcgttgaa actgcacatt atggttttca aacaggtagt atttagtcca
     8281 ctaatgacaa ctaaagagga ggcagaagga tgagtgaaga tgaatacttt gaacagtagt
     8341 ctattaacga cagagatttt taactggaaa ggggtgtagt catggacgca tcggaagtag
     8401 taaataaaat taatagtctc aaagaggttt tcggagacgt cgaagtagtg ataaacgcac
     8461 aaggctatga caacgaattt tttaagcaga ttaccgacat tagcattcag caaggaatgg
     8521 aagacgaaga cggaaacttt atcgatgaag tagccatttt agtgacttgc gagtaagtaa
     8581 ttgacagcta aagaggagga agcggaatga aactaaaaga cggattttac tccagcggcc
     8641 acggcatcgg cggtttaatg ctagatatgc cgacaaagaa tcctaaaaca cgtaagaaac
     8701 caaaattcaa agtcggtgac atggttcgct gcgaagcaga aggattcatc tatccatttc
     8761 gtggatatgt agaacacgtc tataatcact cagcaatcat tcgcattgaa aacacgatgg
     8821 aatgtgataa gtggttagcg aaaagcaaag agaatttagc agtggtgaga ttggtggaca
     8881 tggaagttat aaaatcttaa ttttgctttt ttacaactat cctacaacaa tgagcgttat
     8941 tccacaactg gaaggagaag aggcaattgt gaacatttta gagaatatag atattaagca
     9001 aacaaggaaa aatgctagac gattactaaa aagatacaga aatttagaac gtttggtcgg
     9061 accagtgaaa atagactttt ccgtgatgac tgttaccaaa aatttgaaat tcacaattga
     9121 cagtcaaaac gaagaaatca ttgaagcgat aagtactcga gattcggtta tcgaagcatt
     9181 aacacggcta agtagaatcc atttccaggt actttattat agttattgtt ttcctaataa
     9241 gatgtcaatg tatcaaatag gagaaaaatt aggatattcc gatagaacta tcgagagaat
     9301 gaaggcagta gctttggttg aatttgctga ggcgtataaa tcaggagaac tcatttcacg
     9361 tacaaaataa aaaaagtcgg attcctccga ctatgaataa tatttccgac ataagtatta
     9421 tatcataatt gggggaatca gaggatggta cttttcgatg taaagaaata tgagacacca
     9481 agcgcgaaag atgttgatat ggaacgcaca aaacataatg tcgctgtgtt tctttcagca
     9541 tatctatcag ctagatgtag agtagggcaa catcgagaac caaaagtgac agcatcttat
     9601 tccttggttc caccttctac agctgatcat atatttgaag cagaaagaat gctgatcgat
     9661 aaagaagaag cacaggaaga atttgagtat ctgcataaat tgtttgttcg aggctattca
     9721 gcgatacagc atccgcataa gcctgatgtg actgaaaggc gcaagaagat attctatgat
     9781 cgttatatca atggtctgcc catttatgta actgctcaaa ggaataatac tagcgaagaa
     9841 tcggttaaag tagaatcaaa cagaattatc atccaatttg cttcatcgtt agaactggtt
     9901 gctttcaagt agccagtttt tacacttttt ataccctttt attaccaatt tggtttccat
     9961 tttatacctt ttttatacca atcacttacc tattcaacgt tgtattatga tagtgtcgaa
    10021 aaattaggaa acaggatcga caaaataaaa tgtaagggag gaaatctccc tcatcgttta
    10081 attaagcttc gataggcagc aacggaaata ttaagaataa ggatgtgaat ttcaactcct
    10141 tctaaattgt tcttattatc tatcatccgt tgctgtctat tgttttttta attattcaca
    10201 cgataactaa aggtaagtga agagaaatga ttccattaat aatttcaatt tttgcgcttt
    10261 gtcttaatgt atatatgatt ggatttaaaa atgggcaaaa taaaaaatag tagcagccaa
    10321 gaataatttt atagtgtcac tgtggcggaa agggtagacg ctaagcatgt gtgctaggtc
    10381 aatgcttcgg caaccatgca aggttcgatt ccttgccagt gactttggca accgaggcat
    10441 cggcggttta aaaatatagg ggtgcgcaat ttcgtacgcg ttttgtgcat cgtgtaggtt
    10501 gctattacat aactggttag gttagattga gatttgggat tcggtacaaa tgaatcgtca
    10561 aatgtctcaa gcacaggatc ggaaacgtcc ctgcctgtgc attacatatt agatcactct
    10621 ttgagtggtc tttttcttta cataaaggag gctacataat gagaaactac tggtatgtat
    10681 cgctaactaa tgaatatcct cgaaccattg atgattgttc agtgcgtgtt gtgcgttctg
    10741 tacaaatcaa agggaagtac tctattgtcg aaatggggag agaagcagaa ccatgtgaga
    10801 ttgatgcgtg caagctcgtt tattgcggtc atggtttcta tgatgaacca aacattcaaa
    10861 ataatattaa caagaatttg agggattaga atgcaagaaa tggcttaaaa atctccaatc
    10921 aggacaaatc aagttagaca aagtttccga tttaaagata ttgattgaag ctgatttaat
    10981 gctaaaagat attgaaaatt agaaaacaaa actcaaccta aacagaatgt gaggtggtgt
    11041 gtattgaatg gcaagacaac gtgatccaag acgtgatgaa gccaaaagaa tttggttaga
    11101 atccaacgga gaaaagcagt taaaggaaat tgcatctgaa ttaaatgttt cagattctca
    11161 ggttagaaaa tggaaatcgc aagacaaatg gagcgctgaa ttgattagta acgttaccaa
    11221 tggcaaaagt aacgttacta atcaaggtgg cgctcctatt ggtaatcaaa atgctaaagg
    11281 taacaaagga aatagccgag cttctccgcc agtgggtaat aaaaacgctt tgaaaacagg
    11341 tgagtatgaa acaatatttt ttgacacgtt aaccgataat gagaaggaca tctattctag
    11401 tttgaatgat gatccttctt ttgctttgtc cgaagaaata cgacttctta aaataagaca
    11461 acttcgaatg atgaaaagaa tccaacaagc tgaagctgga ctaaatgatg aagaagtcga
    11521 acgattgcag cagctaagaa agattaaaac tccgattgaa aaaaatggta aaaagctaga
    11581 aatcaagcgt gaggttatgc aagatgtgca gattagcaga aaaaaacatc gcaaaattga
    11641 tgatattctt tcaattgaag attcattgac tcggattagc aaccagttag ctaaagccat
    11701 caagcaaatg aatgaacttt atatgaatga atacagaact gatttaatta aagctcagac
    11761 tgataagatc caagctgaga caaatgaaat tggcggaaat aattcaggtg aagaaataga
    11821 agaatggaaa caggcagttt taaatgccgc aaacaaacgg gcggtgaaag aaaatgaata
    11881 atgaatttat tccttttgct gatattggtt ctgccattga ttattactat gataaaccag
    11941 tagctttttg ccaagatatt ttgcatttga atcctgatga atggcaagaa aatgttttaa
    12001 atgatttagc tgaattttca aaggtttctg ttcgttctgg tcaaggagtt ggaaaaacag
    12061 cattagaagc aggagcaata ctttggttct taacgtgtcg accctacgct aaagtaatag
    12121 caacagctcc gacaatgaag caactttacg atgtactttg ggcagaggta gctaaatggt
    12181 taaatgatag cttgatcaaa aacttactga agtggacaaa gaccaaaatt tatatggttg
    12241 gtgattcaga gcgttggttt gctacggcta gaacagcgac taaaccagaa agtatgcaag
    12301 gttttcacga ggaccatatg ttgattgtgg tagatgaagc ttctggtgtg tctgatccaa
    12361 ttatggaagc tattcttggt acgctatcag gttttgataa taagctgttg atgtgtggaa
    12421 accccaataa tattgaaggt gtcttttatg attcccataa ttcagaccgt gataaataca
    12481 gagttcataa agtatcaagc tatgatagta aacgtacaaa caaagacaat atagaaatga
    12541 ttcttaaaaa atatggaaaa gaaagtgatg ttgctcgtgt ccgtattttt ggagaatttc
    12601 cgaaaggtgc gttggattca tttatcagtc ttgaaacagt tgaattggct acagaaaaac
    12661 aaattagtga ttctttagtc aataaaacaa aggttgctca tattggtgtc gacgtagctc
    12721 gatatggtga tgattctacg attctctttc ctagaattgc taccagggca ttggagtatg
    12781 agaagtattc aaaacgtagc accatggaaa caacaggata tgtcatcaac atggccaaga
    12841 atctaatgag tcaatatccg agtattgata aagtgatgat taaagtcgat gacactggtg
    12901 tcggaggtgg tgtaaccgac cgcctagaag aacttataga agacaaacat tatccttttg
    12961 aggtgtttgg agtgaataac ggttcaacat cagaagacga tttttacgat aatttaggta
    13021 ctcaactatg gggaaacatc aaggaaatgt tagaagaaaa tatgacagca aatcttaacg
    13081 gagaacagcc tgttattgaa ttgccttctg atagttcgtt aatcaaagaa ttaagtactc
    13141 gcaaattcaa aatgacaagt agaagtcgta tacgtttaga aagtaaagat gatatgaaaa
    13201 agcgaaatat tggtagtccc gatattgctg acgcactggc tttagcgttt tatgagccac
    13261 caagtcacta tcaatttatt caattttagg aggtgagctt ttgattagta cagtattatc
    13321 tttagagaga tataagaagc tacgtgttaa atatgcaaca caaattgaag atggaatgtt
    13381 tgatccgaat ggttttatag aagatatgaa accatttttt gctgatcgcg aaagaaaata
    13441 tctagcttat actagcgaaa agaatgaaat agataataga ccaaagccta acactgatat
    13501 tgtaaaagtt aataataaac ttcatgctgg tatgtattca attgtcgtag accaagcagt
    13561 caatcatttt actggtatac ctatcaaatg ggattacgat gtatcggaac aaaaaagaac
    13621 actcattcaa agattaaaag ataaattctt aaaaaatgat atagaacttc cgacagttcc
    13681 agaagctttt aacaaattaa caagcaatct tgattccatg agattcgcaa tgcttgattc
    13741 tgaaactgca acttttcaag gagcttgtgg agtagctttc cgattgctag agcctgtgga
    13801 agaagatgat ggttggaagt taagagcaag taatattgaa ccttggagag cagaaagata
    13861 tggaaatgcc ggaatctata tcaaagaaaa gtatgactct taccagaaaa aattttttca
    13921 agaaatgaaa gttatgacaa gaaataaaat tctgacatat gcttgctatg gtgatttgaa
    13981 ttttgttaca agcggaacgt tcaaaaaaat tgatgagacc gataaccctt tggggacgat
    14041 taccttggca gaatttaaaa ataatacgaa tcgttattgt gattttgaag tagctgagga
    14101 aattggagat gcaattgatc gtgctttgtc tgatcaacaa aacgaaatcg aacaatttaa
    14161 gcttgcgtac atgcttgtta ctggcacaac aatgagcaaa ggcactgcta aagaaatgat
    14221 gaatcagcta gggattatca acttaaaagc ccctactgca aaagctgaat atgtcacaaa
    14281 aaatttagca aaagatttca atgaatatca tatggatcta ttgaaaaaac agttttacac
    14341 catttgtaaa gcaatcgatt ttaacgatga ggtgtttaaa tcaaacagtt ctggagaagc
    14401 tcgcaagtgg caaatcatta gcctagaagc gaaaacaaat actaaagagc aatattttag
    14461 agaaggattg aaagaatgcg cagaaacaat tgctgctttt cttaagtttc atgacaaagt
    14521 agaaattgag cctgaaaaaa ttatttttac tttctctaga tctttgccaa ctgatttaag
    14581 ttatttagct gaagctttac ctaaacttgc accatacgta tccaaacgaa ctattcagag
    14641 ccaaattcca tttgtaacag atgttgatta tgaaaatgaa atgatggaat tggaaagtgg
    14701 aagtgcttat ccagatagtg aatacaattt tggcggaggt ggcaatagtg acgatagaaa
    14761 cgaagtattg gaccaaacgt cgagaactgg aggatcaagc aaggctcaaa caagaaaatc
    14821 agacacttaa aaaattaact agtgtatttc ctgaagcact gaaagagata caagcaaaac
    14881 tattatcaca agctgactta cacaatatca cttatccaga aatgatggag ttttacagta
    14941 caagtaatca gaaaaaatat cgtgaatatg tggaaaaaaa ttataagtca ttaaaaatgt
    15001 atgatgcaaa atacaaagag tttatcgatg aattttttcc accatttgac tatgcaaaag
    15061 tcaatcgctt attacaaata cgatcagatg tatttaaaat tcttgcagaa tatgcgatgg
    15121 atgcagatgt gaatcaatat ttttctgatc gcttagagga aattcttcaa agaacatatt
    15181 cttctaatgc taatgttttt gttcaacttt taaacgttga tataccaaat tatttaccag
    15241 aaaatgaaat taaacattat ttgaactatc catggtcagg aaagacattt tcaagaagac
    15301 tttggggaaa tatttcatcg cttgaacaga aactctctaa tgctattgta aaaagtgttg
    15361 ctagtggaga aggcgttata cacgcattaa acaccatgag attagactca gaaatttgtg
    15421 acatgtttaa gttagaagaa tcaaagtata acaaagcgat agaaaatctc gttcgaacgg
    15481 agtatgcaaa atttgcacaa gatggtattg aaaaatcata tttagaaaca ggtattgagg
    15541 aatacaacgt attgactgca aaagatgaga gagtttgccg gatttgtgga ggaaaggcaa
    15601 gtaagaatcc ctataaactg aaagatgctg tcataggtga aaatcgagca cctttccata
    15661 gtcgttgtag atgtacggat gttcctaatt taccaaaatt aggaaaggat attgatgaag
    15721 aatatgaccg tttatttggc gatttattag atgagtttgc acatgattct tttggaatta
    15781 atttgaaacg gaggaagtag aatgaaagat ttttttgaag cagtactaac aattaatgta
    15841 aatgctgata ttgcagaagc ctacaaaaca gctattgagt ctgagaacca tcctaatggc
    15901 ttgagagacc attggaatgg caattatgcc tacgtggtta ttggcgatca aactgttaat
    15961 tatcaagata atactccagt tgataagaat accgttaatt taacgattca attattatct
    16021 cattcattac caaatttaaa agaaacagtt gattggtatg aaaagatggg atgtattgtt
    16081 gttagaactg actacaaaga aggaaagtct agtaattagg cttttttatt ttgtccgaaa
    16141 tgacgttaaa ctagcgcaat actgggcttg gttgaatggt ggggcgcaac tattaaaact
    16201 caaagcaatg cggggcgtga aaacgaatcg tggggcgaaa ggagaatgag catgaaacac
    16261 aaatcattaa tgccaatgaa tttgcaatac tttgccgaag gtgacgatca aaagttttct
    16321 tttgatgact tcaaatcttt tgtggaatcc aatgaagaag ctcaaaaatt tattcagtca
    16381 caatctcaaa gtgttgccga caaacaattg gaagcttgga aacaaaataa cttagataaa
    16441 attaaacagg ataccatcaa ggaatatgag gaatctaaga aaaacaagtc acctgagcaa
    16501 attcaactgg aaaaactaca agctgaattt gaagcagaaa aagcgttgcg tgtgacaagt
    16561 gataataaag catttgttgc agaacaaatt gctggattag aactagatgg agagttaaaa
    16621 gagtctattt ctcaatttat gctaaataat cttgttagtt cggatacaga tttcactaag
    16681 aatgctgttg aaggttttac aagtgttttg aatgcaatta atgagaaaca tgcagatgca
    16741 ttaaaagaac tacaaatgaa gtctgcattt ggtggaactc aacaatcgaa taaccaggtt
    16801 cagcagaaca atgaaacatt aacaaatcca gaagaacaat taggacaaat tcttcaacaa
    16861 tttaactagg agagtgaaaa attatgaaaa aaacatcttt aaataattta gagtatttgg
    16921 atatttcacc agcgattaat gctatgcaag taccaaatac acctttttta agctatttac
    16981 ttggtgctgg aaaaacagag ccagcaaact cgacagaaat taaatggcgt gaatatgata
    17041 tcaacaacga tgattcttct gaaaaacttg agggcggaga atatccagat gctgaatcag
    17101 gtcgaacttg gtttaacaac tatactgaaa tttttagaaa atcaacctct gtatctggta
    17161 cattagatgc tattaatgtg aatggagtcg gaaatgaatt aactaatcaa gtagcactac
    17221 gtggtatgga aatgaaaatt gatttgaacc gaaaattgat tactggtgta aaagctgatg
    17281 aaaatggttc taaaggtcgt cgaatgaatg ggattttgaa cttgatcaat tcagcaaata
    17341 aggcagaaac agccactgcg ggtgcagtaa caagaaaaga tatcgatact ttatttaaat
    17401 tgatgtatga aaaaggttat atgggagaaa aactatgctt gatttctcca gatatgcagg
    17461 agttaatgac tgatgagtta gatggaaaat caacaaaaat tgttcagttc ggggaaagag
    17521 taacttttgg attgcaaatt ggaaatatcg tgtctaatta cggtacaggt attgctctac
    17581 tagaaccatc attgccaaaa ggaacaattg ccgcaatcga tactaattat gtgaaactac
    17641 gtccattacg tgaatggaga gcagaagaac ttgcaaaaac aacggattct agacgtatcg
    17701 gtcttgtagg tgaatactct cttgaataca atgcttcaaa ctctggggca attttaaatt
    17761 taaagtctga ataaaaggga gttagtactc ccttttttga taggaggaat tacagtggta
    17821 aaaaaagatg aaactaaaaa agatgaagtc gtgaaatata gagtaggtaa aactaaaaat
    17881 tttgttgggt ttgttcatcc taaaactcgt agatttatca cagcagattc aaataacgaa
    17941 tttatcattt ctatagatga taaagaagca attgcaattt tggaagatgc aattgatgtt
    18001 aatgaaattt aggaagtgat ctgatggatg aatcgctaaa aacggaaatc attgagtcta
    18061 caaaagaaga ttttccagat ttgagtgaag aacgcataac taatttatta gaaataattt
    18121 tgctagaaat tgaatcatac aacacttgta aaaatgatat ttcatgggaa aagttaaaaa
    18181 gcgtgattag tgaagtgttg tataaaataa taaaaaatga atcagaaaaa acagtatctt
    18241 cagttagacg tggcgataca acgattagtt atgcttcaac aacaaatgat gttagtgagt
    18301 tacttctagg ctatggtgat ttgatacgaa gagttattgg ttgtggagga ttggagtttt
    18361 tttaatgaat gaagcagata ttttagaaac tacttatgaa gatagttgta ttattgaaag
    18421 actgacggac attgaagatt ctaatacaaa tattactatt caagattaca aaaaagtata
    18481 tgataatcct atttcttgcg ctctttcaca aggtcaaatc gatggactag cagtcataga
    18541 agatggagag atggtaaatg tttcaactga cacatataaa ttatttgttc atcctaagat
    18601 taaactaaaa aaaggagatc gaataacaat aactcaaaag gccagtggct taattttttc
    18661 tctatttgct actaagcctt tttactatcc tagtcattgt gaagtgaatt tgataggaag
    18721 tgagaaaaat gggtaatctc aaatatgaat caaatgcaga aaagatcatt gagaatttta
    18781 aaaatatgac tgtaattgct caaaaagaag gaatatcctt cgttaacgat tcaatgaata
    18841 aagtcgttag tttagctaaa ccattaactc ctgtaaaatc aggtaattta aggcgaggat
    18901 atcgtgtagt aaaagctaga aaactatcaa gcggtcgaat tgttggagaa gttataaaca
    18961 atgaacatta ttttaaatat gtagaagaag gtcataggac taaaaatggt ggctttgtca
    19021 aagggaaatt catgttaact cgtgcaacga atcttgcaaa tatgtcttat attcctcgaa
    19081 gatttaaaca aatggcaata aaaatcgtta agaaaggaaa gtaacatgaa agatgaaatc
    19141 attgctgcaa tcagtagcaa attaaaagaa atctatcctg atgggacaat atatcttgat
    19201 tcagttatgc agtcaactaa agatttttat ttcgttttat cagtaatgga atctggaact
    19261 gaaaatgtag gaattgatat tcaaaatgtt tctttcttaa ttgatattgc attgattgat
    19321 aataacccta atagaaattt aattaatgaa ttagtctcac gttgtgggac tttttttaat
    19381 acgattacaa tcgatgagca aatattattt ccaaaagcct atttacctga tgaagcagat
    19441 ggtgttcaac atattcgttt tacattagaa tttccacaat atattgaatg gagtgaaaga
    19501 taaatgggag aaaaaagaag taaaaccgga attatttctg tcgaaaaacc tacttggttt
    19561 cctttggaag atgagacagg gaattttccg gtatatggta ctgcgttgcc aatgggaaca
    19621 gcagtaagta tcaaaccaac agctaattat gaaacaacac aagattatgg ggattcagtg
    19681 gttcaagatc aatttacagc ttttggtggt gcagaagttg aactggaagc aaatggatat
    19741 actcataaag ttttatctac aattactgga gggaaaattg ttaaaggtgg tgccttgcgt
    19801 tctggagaag atattgcgca agatggtgcg tttgcatata gacgtaaaaa atcgaatggg
    19861 aaataccgtt atacggtctt ttataaagga cagtttgctc tagattcaga tgaaacatca
    19921 acgattgaag gaagtaaagt aagttttact catccaactt ggaagggttc atttgtagat
    19981 gtaccaggac ttggatatat gtattcagtt gatgaagatg atgaaggcgt ggataaagca
    20041 atgatcggaa attggtttac aaaagtagca attccaattg aagaagcaga actttcagga
    20101 ggtacagaat aatgtcaaaa tatcaaacaa cgattaaatt aacgaaaaaa gacgaagaag
    20161 gtaaatatga gcaagtacaa tttaaatctg ctgaattttt accaggaaca gttgtagaag
    20221 atgctgcagg agttatggaa gaaatgcaaa cagcgactga taagcaatca gttaaaaaag
    20281 ctttaagtcg tgcttattca tttattgcag atactttatt tgagggacaa tttactggtg
    20341 aagattattg caaaggaatt gatgctcgtg agattgcctc attgacagga aaattattga
    20401 agtctgttac tgcaggtttt gatgaaactt atacagagac gaaaaaaaag taagtgaggc
    20461 gctcaagtca ccttcattta agtattcgat tacttaccga gaattagata taaagacgca
    20521 attactcgaa gcaggttgga cgttaccaga aattgaacgt actgacttgg atgagttaat
    20581 gcgtcttttt gcttttagag atgcggtaaa agagcatgaa gatgttgagt actatgataa
    20641 tttcactcaa ttttaggagg tgatactttg aataacgatg acctaattct gaagatgata
    20701 ctagatgaat ctggttttac tgccggtatg aataatgctg tcaaaaagtt gaactctttt
    20761 gatgaaacaa tcgaaagaaa aagtagaaat agtggcagtt cattaggtag tatttggaaa
    20821 atatttgctg gaagttttct agctagcgga gtaactagaa tcgtaggagc tggctttgat
    20881 ctaatcaagg gttccataag tggagcggtt gatcgagtag atacgatgaa taacgctcta
    20941 cgaaatttcc aaaatatggg atttagtaat tcagaaatta tgaagaatat cggaaagaat
    21001 gggcttttat ctcaaggtat tcaaggactt cctactgctt tgaatgatgc gataagtcat
    21061 gttcaacttc ttgcttcttc tacaggtgat ttaactcgtt caactcaaat atttaaagct
    21121 ttaaatgatg gaattcttgg tttcggtggt tcaactgatc aagttaatga agctgttatt
    21181 caattatctc aaagtttctc aaacggaaaa gtagacgcac aaacttggaa ctcaatgatc
    21241 aacgctcaac ttggacctac tctttctgct attgctaaaa agatgggaat tacaatggga
    21301 gatctgaaag aaggtttatc tcaaggtaag atttctgttg aagaattcca aaatcaatta
    21361 atagaaatgg ataccaaagg tggcggagga cttaaatcat taagtcaaat cgctaaagat
    21421 tctactaagg gaattaaaac ctctatacaa aatgcaaaaa cagctgttac acgtggtgtt
    21481 ggtgaggtaa tagaaggatt aaataaagca ttagtagatt cagatttagg tggatttaag
    21541 ggaattattg ataaagtggg cagttcaatg gaatcgttct taaaagtaat tgctgcaaat
    21601 attcctacag cagtatcatt tttaagtaat ttatttaacg aaattcaaaa atttggttct
    21661 gctttgaaat tcatgatgcc ctttcttgtt cctgcagcta ccgcttttgg agcgttcatg
    21721 tttcaactta aaggtatacc agcaataata aaaagcttca ataattttaa gaatgccata
    21781 attggcgttg gaaattcatt aaagattatg ggggcaatag ctgctgctaa tccatttgtt
    21841 ttaattattg gtgcagtcgt tggtgcaata gctgttttcg gttactttat ggcaaccaat
    21901 gaagagctta gaaacaaggt tatatctatt tggaatgatg tgaaagattc cgtaattggt
    21961 gtattaaaaa atataaagga ctggggaatt gatacttgga attctgctaa agaaatggca
    22021 tcaaatgcag ttgagggtgt caaagatgct tggtcaggaa caaaggaatg gttttctaac
    22081 acctggaaag gtattaagga tggggccaca ggtttatttg ataaaacagt agagacttca
    22141 agaaatgcag tcgatagtgt aaaaaatgca tggtctaata tgaagaaatg gttttctgat
    22201 acttggcaaa gtataaaaga ttcagccaga gaaaaatgga atggaatcaa aggttctatt
    22261 atgtcagttg ctggtccatt gattacaggt ataaagaatg catttttaca tgttactttt
    22321 tatctagaga ccttatggaa taatttagtt gagattggta aaaacgtctt tgagatttta
    22381 aaaaatgtta tacttgcgcc ggttttgttt attacctcca tgatttctgg tggatgggaa
    22441 gaaacaaaaa ataatatgat tgctgtttgg aataatatta aagaaagtgc cataaatatt
    22501 tgggaatcta taaagaatat atttgtaagt tattttacaa atatttattt tgctgcactc
    22561 aatatttgga cagggttcaa gcttacattg ataaatattt ggaatgaagt ggtaaatcaa
    22621 gctaaatcga tttggttaaa cataaagtat ttttttatta atctttggat tgatataaaa
    22681 tattttgcaa ttcagaaatg gattgaattg aagttttcta ttattcaaac ttggatcgat
    22741 ttaaaatata atgccattac tacttggaat aatattaaac agtttttcaa agatacttgg
    22801 aaaaatatta aagatacggc atacaatacg tggatttcta taaaaaatac catgattaat
    22861 acatggaata acattaagga ctctttctgg aatattgtta ctggaattgt taattctgct
    22921 gaaaatgcat ggacaaatct aaaaaatggt gtttcaaatg ctattaatcg ggtaaaagaa
    22981 atctttaatt cgttaaggga agtcaattta tttgaaattg gcaagaacat cattgatgga
    23041 cttataaatg gtgtaaaaga aaagtggaat gctttgaaag atactatcaa aggtattgca
    23101 ggtaatatca aaaattggat taaaggagct ttagatattc attctccatc aagatggatg
    23161 cgtgatatgg ttggtaaaaa tattgttcaa ggtattatcg ttggtattga taaagagcaa
    23221 agtaagttag atcaaaccat gacaaattta gtaaaaacac cgtctgttca acctgtagta
    23281 aatggaataa gtcacctacc aattacacaa acaaagcaaa atatatctgc tagtgaaaca
    23341 aaagaaattc atttacattt aaatgtttat ggagatttac ctgattcaat ggttcggaag
    23401 attaccacaa aaattaaaac agaattgaca agacaaatga aacgagatgc tggtgcagta
    23461 ggaggaacat tatatgcaac ttaagagagg acaatttttt attaatcaac attattcttc
    23521 tgaatttaat gtgtatattc aaaacaggcc tgcttctgtt tcagctagtc gagtaattga
    23581 attgagagag cgagaaggaa atgattctat cattattgat aaaggctatt acaaaaatgt
    23641 gactagaaag attgagtgtt actataaagc accttcaatt gatatggtgc aggagtggga
    23701 agatcgaatc actgaatggt tagatatgtc gtcttatagt gattttattc tttactatga
    23761 tgaacaatat atttatcaag ctgtagtaat agaaggacct gaatttaaag gaacaagaaa
    23821 aacaggaaat attgtccctt ttgaatttac agtgagtatt cgaccattta aggaaaatta
    23881 caatggtaga tttactattc gacaaacaga aacttttgaa atttataacc cagagaagta
    23941 ttcttcaaaa ccgcttatta aattgagtgg ttctggagat gcttcttttt atattaataa
    24001 ggataaatat gatttgaaat cgttggacag agaattattt atagattcta aattagaaga
    24061 ggcttatcga aaattagatg gtaatttaga acatcaggat caggtcacct tatttttaga
    24121 ttttccattt ttatatcctg gaaaaaatga aattaaatgg acgaaaaata ttcattcatt
    24181 tgaaataatg cctaggtggt ggagaaaagt atgaaaccaa gaatatatag tcctactgaa
    24241 acagatttct caacgaatgg cttaggaatt ttaaaggatt gtacaagatg tggaatatat
    24301 gaagtagcta atggaaaata tgaattagag ttggattatc ctttaggaac tagatttgat
    24361 gaatattttg aaaatgacta tcaaataaaa gcaaagccaa acgatcaaga agagtatcat
    24421 atctttttta ttgatgataa agatatagat acttttttaa atacagtaac tatttatgct
    24481 cagagtcgta caaatcgact tggaagacgg gtagtcactc ttgtggaagt ggactctaaa
    24541 actggtcaag aagcgatatc aattattgaa actaaaatgg ataaaaaatc tgacatacga
    24601 ctttattctg atattacggc tgtttctagc acaatctttg aagcgagaaa tgttttaaat
    24661 tgtattgctg gtgaacaagg atcattgctt caatattggg gcggagaaat taaacgtgaa
    24721 ccatttaagc tttctttgtt aaagcgaaga ggtcgcaata atgttggaac gattcggtat
    24781 ggaaaagata tgtctggttt gaaggtcaaa ttagattgga caggtataaa aacaagaatt
    24841 attccatatg ctgatcctca aagtgaagta ggtacgacta atcgaattta tggttcgcca
    24901 gtagatagtg cctatattaa taactatcct gatgtctata cagaacatgt tcagtttaca
    24961 gaagaacaag gagtaaaaga cattaagagc ttaaataaaa tagctaaaaa ttactttaaa
    25021 acgatcaatc ctggctgtga taagcctaaa gtttctatta cggttgaatt tgataagttg
    25081 actgatagtg aagaagcgaa agaatttgcg aagattagaa attatgg
//
LOCUS       DAZKLM010000034        24943 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3764
            SAMEA115706913-rid22582563.denovo.034, whole genome shotgun
            sequence.
ACCESSION   DAZKLM010000034 DAZKLM010000000
VERSION     DAZKLM010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706913
            Sequence Read Archive: ERR13196647
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24943)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24943)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287823.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287823.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,004
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,849
            CDSs (with protein)               :: 2,849
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 41 of 96
            Pseudo Genes (incomplete)         :: 52 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 15 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24943
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706913-rid22582563.denovo.034"
                     /isolate="VRE3764"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..497)
                     /locus_tag="ACG842_001294"
     CDS             complement(<1..497)
                     /locus_tag="ACG842_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISEf1 family transposase"
                     /protein_id="HFQ0247848.1"
                     /translation="MNDFTTEIVQTLVTKGDLNELFRSHLEKAINTLLRTELTAFLDY
                     EKYDRTGFNSGNSRNGSYFRSIKTEYGELTLEIPRDRNGEFKQQTLPAYKRTNDTLET
                     TIIHLFEKGVTMSEIADLIEKMYGHHYTPQTMSNMTKVLTEEVNAFKSRALNDKYVAI
                     FMDAT"
     gene            complement(738..3083)
                     /locus_tag="ACG842_001295"
     CDS             complement(738..3083)
                     /locus_tag="ACG842_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HFQ0247849.1"
                     /translation="MPRKNTRKKQNRRKKEKWFVPKIIFRVFQSLTVFITVLLLLFAA
                     LGIGIGAGYFAYLVEDTQLPTKKELQTELGNITETSKIVYADNTEISKIQTDLMRTTI
                     SSDKISPLLKTAIISTEDEYFDKHQGYVPKAVLRALVSEATGIGSSGGSTLTQQLVKQ
                     QILTDETTFKRKANEIVLSAQVEKHFSKNEIITTYLNVSPFGRNNKGQNIAGVQEAAR
                     GIFGVDAKDVTLPQAAYIAGLPQSPITYSPYTNTGALKEDLSAGLARKDFVLFSMYRE
                     GQITKEQYEEAKAYDLTKDFLPQQIAEQTDREFLYYTVMNEATRIIAQQLAEKDNADM
                     SDSSVSEAYYQKAQQTIQNKGYTIHSTIDKDIYAALQTGVENYGYMLDDGAGSQVETG
                     NVLMDNRTGRIYGFVGGRNYSQNQNNHAFDTERQAGSSIKPVLVYGPAIDMGLVGSES
                     RVSDYATTWQEGKNAGEKIVNATNEGSNTFQTVRESLEWSNNIPAYHLYQDVLNSGGS
                     KQYAYEHYLAKMNYPANDNWGVESAPLGTVDVTTLQQTNGFQALANGGVYEEGYIIDS
                     ITDNAGNVIYKHESNSVRIYSEATASIMNDMMRSVINEKITTPFKDVISSLNGNLGNA
                     DWVGKTGSTNEYRDSWLIVSTPSITISSWAGHDDNTGMDSQARIRSANYLANLINQAY
                     QANPEIFGTDEKFDLSSDVMKKKVSGFTGQTAGKVTVNKKSIQTPGKTVESLWAKNGP
                     KKSAFKFGVGGTDENYTDYWNTASAYARANPETKESDDKDD"
     gene            3362..4264
                     /locus_tag="ACG842_001296"
     CDS             3362..4264
                     /locus_tag="ACG842_001296"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HFQ0247850.1"
                     /translation="MLLGSHVSMSGKKMLLGAAEEAASYGSSTFMIYTGAPQNTRRKA
                     IEEMNIDAGKAFMAENGLTNIVVHAPYIINLGNTIKPEMFPFAVQFLREEIVRAEALG
                     ATQITLHPGAHVGAGAEAGIARIIEGLNEVLTKEQTAQIALETMAGKGTEIGRTFEEL
                     AQIIDGVKLNEKLSVTFDTCHTNDAGYNIKEDFSGVMEEFDKVIGLDRLKVLHINDSK
                     NPQGSHKDRHANIGFGTIGFDTLNKIVHDPMFDNISKILETPYVGKDKKKAYAPYGKE
                     ISMFKAQAFDPEVFPELLAEEVTL"
     gene            4475..6250
                     /locus_tag="ACG842_001297"
     CDS             4475..6250
                     /locus_tag="ACG842_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyltransferase"
                     /protein_id="HFQ0247851.1"
                     /translation="MLNILNPLIDQLKKKHTYFILGIAVLFTIIRLYLYSKAIYGTDM
                     SATYDDQLLIHYAQNILDGKWLGEYTTTTLSKGISYSLFLVLAHKLHLSYAVLFGMFS
                     IFASILVALAFKPIVKNKYLLLSIYFYFLFSPISFTHEYSTRTYRNALVIPAVEIIIA
                     CLLAIYYRKYGSSRKLLPWFLGLSVAFPFFWFIREDSIWLLPFTVVALGISFVQILLG
                     QDFSFKEVKKLKKRLFLLSKREWLKLSLFLLPIVGFSIVYQWIEYKNETTYGIATIND
                     RSSGEFGRLMKQLIRIDDETNLNKENSEIWVSHAALNKALAASPTFHTVSTRIDELYT
                     THAWTQGGKVKELPGDIIFWALRDVVSESGYYQNNAKETNDFWKKVNQELEEAYHKGT
                     IKKKKEFYLMGSGDGKTMSDLPIVFDFLQSGYAYAWRYKDFHQGGNYSFGTQEQVKQA
                     QELLNLPLMENWIDSNEPRTPNFQLTKSAKVANVIIKIYQVLAIPVLIASIAGILLMI
                     LGTVFAKADRAYYSSSLIVVTGLILTQLVFLFGVSWFCSYAPEKKDYFLSVYTGAGVP
                     LTQFIVVTSMLVFSKLNLKKALSSK"
     gene            complement(6316..7902)
                     /locus_tag="ACG842_001298"
     CDS             complement(6316..7902)
                     /locus_tag="ACG842_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HFQ0247852.1"
                     /translation="MNNQSNKQFHKHRLIEHTNGLSLAEVNGTVDVPKGKSFFKTLLA
                     YSGPGALVAVGYMDPGNWSTSITGGQNFQYLLMSVILMSSLIAMLLQYMAAKLGIVTQ
                     MDLAQAIRARTSKTLGVILWILTELAIMATDIAEVIGAAIALYLLFDIPLVIAVFITV
                     LDVFVLLLLTKVGFRKIEALVVCLIFVILFVFVYQVGLSNPDWHSLFAGFIPNSHTFS
                     DSPVIGGETPLTGALGIIGATVMPHNLYLHSAVSQTRKIDHQNEEDVAQAVRFSTWDS
                     NIQLSMAFLVNALLLIMGVAVFKTGSVQDPSFFGLYEALSDPSAMSNGILIAVAKSGI
                     LSTLFAVALLASGQNSTITGTLTGQVIMEGFIHMRIPIWLRRLVTRLLSVIPVLICVL
                     FTSTKGTIEEHAALNNLMNESQVFLAFALPFSMIPLLMMTNSQAEMGSRFKNSFIVKL
                     LGWFSVISLTYLNLRGLPGQIESFFGEQATSAQLALADHIAYVIIVLVLLLLAWTIGD
                     LYKGNKKYEQLLQETVEEIE"
     gene            8153..8377
                     /locus_tag="ACG842_001299"
     CDS             8153..8377
                     /locus_tag="ACG842_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HFQ0247853.1"
                     /translation="MVSTGIVVLIAIIALLIGAVGGFFLARKYMKDYLEKNPPVNEDM
                     LRSMMMSMGQKPSEKKIRQMMQQMKNQGKK"
     gene            8519..10270
                     /locus_tag="ACG842_001300"
     CDS             8519..10270
                     /locus_tag="ACG842_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HFQ0247854.1"
                     /translation="MSIFRKLGWFFRQEKKHYLIGIFSLFAVALVQLVPPKVIGIIID
                     EIADKNLSIKIISWWILLLILAAFLQYLFRYIWRTNIWGSAARLERELRQQLFKHFTE
                     MDNLFYQKYRTGDLMAHATNDLNAIQNVAGAGILTFADSVITGGTTIIAMILFIDWRL
                     TLIALIPLPLLAVTSRVLGSKLHDAFRDSQEAFSSINDKTQESITGIKVIKTFGQEKE
                     DLADFEEKIEDAIIKNKRVNFLDSLFDPFITLIIGLSYVATIILGGKYVLEGTISIGQ
                     MVSFISYIGMLVWPMFAIGRLFNVLERGNASYDRVNELLHEKTHIVEQLGAVQTPAKG
                     ELNVAISSFNYPKEESPALEQIKFAIQEGETLGIVGKTGAGKTTIIKLLMREYDDYQG
                     KIQFGGRSIKEYSLNALLGSIGYVPQDHFLFSMTIRDNIRFSNPKLSEEEVEHAASLA
                     FINNEIKELPKGYDTLVGERGVSLSGGQKQRISIARALIKNPELLILDDALSAVDAKT
                     EEAILHNLKEIRKEKTTIIAAHRLSSVMHAKEIIVVDQGKIIERGTHHELLALGGWYE
                     RMWEKQQLEAKIEGGEA"
     gene            10270..12054
                     /locus_tag="ACG842_001301"
     CDS             10270..12054
                     /locus_tag="ACG842_001301"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0247855.1"
                     /translation="MDENYQSEWTKSIPLKEQKKIIKRLMKFAKPFRRTFIVAILFAF
                     ALSVINVLLPRIIQTFMDDHLAKQSATTQVILFFAGVYLFGVIVKSIIWFFQWYLYSM
                     ASLKTYQYVRVRLFEKLHTLGMRYFDQTPAGSTVSRVTNDTETLFEFWYVFLMVLTGI
                     FAVVSSFVAMFQINGEIALWTLVFLPILGIVIWYYQKFSSRIYRNMREKLSQLNTKLN
                     ESISGMRIIQQFRQEARLAKEFEQVNNEYLDTRYAMIKTNSILLSPIINFLYALAIAL
                     ALTLFGIDALHSPVEVGLIYAFTTYVQEFFNPMSQMMDFLSTFTDGMVAGSRILKIMD
                     TEEYAPQQNPGADQAITSGKIEFRNVSFSYDGEHNVLNNISFVANPGETVALVGHTGS
                     GKSSIINVLMRFYEFYEGQILIDDHDIREYPMEELRKKMGLVLQDAFLFYGDIAGNIR
                     MMNPAITDEQIRHAAEFVQANKFIDELPKGYHSKVIEHGASYSSGQRQLITFARTIVT
                     DPKILVLDEATANIDTETEGLIQEGLAKMRQGRTTIAIAHRLSTIRDAELILVLDKGH
                     IVERGNHETLLAQNGLYADMYKLQSSGV"
     gene            12389..13241
                     /gene="licT"
                     /locus_tag="ACG842_001302"
                     /pseudo
     CDS             12389..13241
                     /gene="licT"
                     /locus_tag="ACG842_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289170.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator LicT"
     gene            13338..15224
                     /locus_tag="ACG842_001303"
     CDS             13338..15224
                     /locus_tag="ACG842_001303"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
                     /protein_id="HFQ0247856.1"
                     /translation="MGKYQELARKIVENVGGKENINGLTHCITRLRFKLKNEEKANDE
                     ILKNMDGIVTVMRAGGQYQVVIGNHVPVVFEEVIKAGNLTFDEAVSTEKMRPFDMLID
                     GISGCFQPFLAILAADGMIRGLTAFLVFLGAFDRGSGTFVMFDNIGDSVFQFMPVIIG
                     LTAARKFKVNEFVGMLLGAALMNPSLSLEALSGAAEAPLTTIFSGTIFEAPIYQTVFG
                     IPWIARNYASSVIPIIFIVLLASQIQKPIKKLVPEMIANFFVPFFTVLITMPLGFLLV
                     GPVFTFATDILMAGFETLLALSPVIYGAIVGFFWQILVMFGLHWAIVPMGLMQFSVNG
                     WQNIMTPVAVVSFGQTAALTALYFKLRNPKDKAIAIPAIVSGIVGITEPAIYGFTLPR
                     KKIFIFTCVGGALGGAYSGLMNLTSWNQGGLGIFTIPNYIRPDGDLTDVINVLIGIAI
                     AMVVSFALTFFFWKEEADETDDRQKRSGKEIVKTPIQGQIAPLNAAKDAAFAQGTLGR
                     GILIYPEKGEVRAPFDGTVMSLFPTKHAIGMVSEAGLELLIHVGLDTVQLEGKYFESL
                     VQQGERVKQGDLLLRFDKKKIEEEGYSLETPVIVINYMDYLDILENQGETVGPDDALI
                     TALT"
     gene            complement(15265..16167)
                     /locus_tag="ACG842_001304"
     CDS             complement(15265..16167)
                     /locus_tag="ACG842_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFQ0247857.1"
                     /translation="MLLREKIKEISFSPSENEVIQFLLSDSPALGEMTIQEIAERCYV
                     HPSTLIRVAKKMGFDGWVSFRNAFLAETAYLEGNFQDVDANLPFSTTDGIMTIANKMA
                     SLEQMTIQDTLSLIHHDQLQKAKQLMLQAKKIVIFASNSNTLISQDFMLKMKRIKMDV
                     TIVTTFGESFYEAYNCTSDMCGILISYTGENNVILRTAEILKQNHVPILALTSIGDST
                     LSSMSTAALRLTTRERLYSKIGNFTINTSICYLLDVLYSCVFAENYQQNLVHLISVGQ
                     KFDKRPISSAIMKEPFNFENKEVD"
     gene            16397..17800
                     /locus_tag="ACG842_001305"
     CDS             16397..17800
                     /locus_tag="ACG842_001305"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008706 - 6-phospho-beta-glucosidase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HFQ0247858.1"
                     /translation="MAKGIKIATIGGGSSYTPELMEGFIKRYEELPIREIWLVDVEEG
                     KEKLEIVSQMSQRMWDASPYEVKIYATLDREEALKDADFVTTQFRVGQLDARIKDERI
                     PSYYGMIGQETNGAGGIFKAFRTVPVVLAIVEDMKRLCPNAWLINFANPSGMVTEAVI
                     RYGKWDKVIGLCNVPVMAMMTEPTMLGKKKEELIYKFAGLNHFHWHKVADNKGNDRTA
                     ELIDRLYDEDNGLPKNIFDIPFFKEQLKQMNMIPCGYHRYYYREEEMLEHALEEYQTI
                     GTRAQQVKETEAELFELYKDPELDHKPEQLQKRGGAYYSDAACETIASIYGNKETQIV
                     VSTKNDGAVPDLPADCVVEVTAYVGAQGARNVAFGSLPAAERGWLQVMKNMELLTIEA
                     AVTGDYGTALQAFTINPLIRSGKTAKRIMDELFIAHQQYLPQFKETISRLKAEGIEVQ
                     DELARELSSNTHVMEEV"
     gene            17800..19233
                     /locus_tag="ACG842_001306"
     CDS             17800..19233
                     /locus_tag="ACG842_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HFQ0247859.1"
                     /translation="MGFRKDFLWGGATAANQCEGGYDEGGRGLANVDVVPIGEDRFPV
                     ITGKKKMFEFDENHFYPAKIAIDMYHHYKEDIALFAEMGFKTYRMSIAWSRIFPMGDE
                     KEPNESGLKFYEDVFKECQKYGIEPLVTITHFDCPMHLIKEYGAWRSRELVGFYENLC
                     RVIFNRYKGLVKYWLTFNEINMILHAPFMGAGLYFEEGENEEQIKYQAAHHELVASAI
                     ATKIAHEVDPENKVGCMLAAGAYYPYTCNPEDVWASRKSDRENYFFIDVQSRGEYPAY
                     ALKEMERNGIQIKMEPGDAALLKEHTVDFISFSYYSSRVSTTDNELLEQTAGNIFASV
                     KNPYLEASEWGWQIDPLGLRITMNDIYDRYQKPLFIVENGLGAVDTPDENGYVEDDYR
                     IDYLAAHIQAMKDAVELDGVDLLGYTTWGCIDLVSAGTGEMKKRYGFIYVDRDNEGNG
                     TLARSKKKSFNWYKKVIDSNGENLSND"
     gene            19378..20304
                     /gene="trxB"
                     /locus_tag="ACG842_001307"
     CDS             19378..20304
                     /gene="trxB"
                     /locus_tag="ACG842_001307"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0019430 - removal of superoxide radicals
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HFQ0247860.1"
                     /translation="MYDVIVIGAGPAGMTAALYASRSNLSVLMIEQGAPGGQMNNTAE
                     IENYPGFDSIMGPDLAYKMYEGVSRFGTENAYGIVQDIKDHGSHKEVICEDKTYEGKT
                     VIIATGCIHRKLGVPGEEEFAGRGVSYCAVCDGAFFRNKRLVVIGGGDSAVEEAIYLT
                     QFASEVVIVHRRDELRAQKIIQDRAFANEKISFMWDTVVDEIVGNEMVVTGVKARNVK
                     TGNEFDLAADGVFIYVGLDPLTAPFREAGLTNEAGWIPTDNEMKTNIPGVFAIGDVRE
                     KDLRQITTAVGDGGIAGQQVYKYLESLEETTA"
     gene            20517..22244
                     /locus_tag="ACG842_001308"
     CDS             20517..22244
                     /locus_tag="ACG842_001308"
                     /EC_number="5.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HFQ0247861.1"
                     /translation="MSWEQVYEQWVHEENLEENLKKQLNDLGEDPEKLEDAFYAPLEF
                     GTAGMRGILGPGINRMNIYTVRQATEGLARFMDTQGEETKRRGVAIAYDSRHQSPEFA
                     MEAAKTLAHHNIPSYVFESLRPTPELSFAVRHLKTFAGIMVTASHNPAAYNGYKVYGE
                     DGGQMPPADADALTKYVREVSNPLKVEVLSDDEAEHSGLITIIGEEVDAAYLEEIKAV
                     TIDHELVETMGKDLKLVYTPLHGTGKMLGERALKQAGFEQFVLVPEQAVADPDFTTVK
                     SPNPEEHSAFEYAMRLGEKEGADLLIATDPDADRLGAAVRLPNGSYEVLTGNQIGALF
                     AQYILEAHQSRGTLPENAAVLKSIVSSELPTAIAEHYGATMFNVLTGFKFIAEKIQQF
                     EEDHSHTFMFGFEESYGYLVKPFVRDKDAIQALLLLAEVAAYYKKQGKTLYDGLQTIF
                     ETYGYYAEKTISVTMSGQEGSAKISALMEKFREEAPTEFAGAKVIQTEDFKELTRTDR
                     EGNVEKLTTPPSDVLKYTLEENGWIAIRPSGTEPKIKFYIGVKGNSSEDAAEKVAAYE
                     AIIKKMTEE"
     gene            22260..22610
                     /locus_tag="ACG842_001309"
     CDS             22260..22610
                     /locus_tag="ACG842_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HFQ0247862.1"
                     /translation="MEKVVNFRSQSFFKLEVNGLPDTQKTKVEKVSQLFKMLSDPTRL
                     KILLYLKDGEQNVTAITQAVEMEQSAVSHQLRLLRENHVVKSHREGKAILYSLDDSHV
                     LDILNQTLKHVEHQ"
     gene            complement(22704..23564)
                     /locus_tag="ACG842_001310"
     CDS             complement(22704..23564)
                     /locus_tag="ACG842_001310"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, water dikinase regulatory protein"
                     /protein_id="HFQ0247863.1"
                     /translation="MSSDSTEIITFFVISDSAGETATKLAQATMAQYPTVEFNLFRRT
                     FVTDEKTLMQALQDALKEKALVLHTLINQELIEMTRSFCEEHQLFSFDVMTPPISEIE
                     RLTGIKPIRQPGALHLLNENYFKRIKAMEFAVKYDDGKDPRGFLEADVVLLGVSRTSK
                     TPLSLFLANKNLKVANLPLIPQAHIPKQLWEIDPKKIVGLTNNPDILNNIRKERMRSY
                     GLNPDTAYSDIEKIRAELDFANDLYEKLGCVVINVASLSIEETASLILNALDLEDHSY
                     YGTETTEETD"
     gene            23656..24084
                     /locus_tag="ACG842_001311"
     CDS             23656..24084
                     /locus_tag="ACG842_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HFQ0247864.1"
                     /translation="MKKQTLIEQSLETLKNNGFKYTKKREALLTYLVKRNRYVSAKEV
                     FDYMNQEFKGVSYDTIYRNLHDFAEIDLLEETDLNGEMKFRFHCCQGEIGHHHHFICT
                     VCGTTKEIHMCPMNYFEEQLGGCTIEGHRFEIFGRCENCQ"
     gene            24246..24422
                     /gene="rpsU"
                     /locus_tag="ACG842_001312"
     CDS             24246..24422
                     /gene="rpsU"
                     /locus_tag="ACG842_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002884573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HFQ0247865.1"
                     /translation="MSKTVVRKNESLDDALRRFKRSVSKAGTLQESRKREFYEKPSVR
                     RKKKSEAARKRKKF"
     gene            24449..24895
                     /locus_tag="ACG842_001313"
     CDS             24449..24895
                     /locus_tag="ACG842_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HFQ0247866.1"
                     /translation="MSLLSRLNDDMKTAMKAKDKESLQVIRMIKSSIQNEQIKEGHDL
                     TEEEELTVLSREMKQRRDSLHEFEEAGRDDLAEKVKSEIVIVEKYMPEQLSDEEIRQL
                     VQEAITQTGASSMKEFGKVMGAIMPKVKGKADGNQVNAIVKELLQS"
BASE COUNT         7878 a         4452 c         5293 g         7320 t
ORIGIN      
        1 taagtagcgt ccataaaaat agcgacatac ttatcattta aggctctgga tttaaaggca
       61 tttacttctt cagtcagaac tttagtcatg ttggacatgg tttgtggagt atagtgatga
      121 ccgtacattt tttcgatcaa atcagcaatt tcagacatcg taacaccttt ttcgaataaa
      181 tggataatag tggtttccaa tgtatcgttt gttcttttgt aggctggtaa agtttgttgt
      241 ttaaactcac cattacgatc tctaggtatt tccaatgtta attcaccata ttcggttttg
      301 attgatcgaa agtaagaacc gtttctcgaa ttacctgaat taaaaccagt gcgatcatat
      361 ttttcgtaat ctaaaaaagc cgttaattca gtccgtagga gtgtgtttat cgctttttct
      421 aagtgcgaac ggaataattc atttaaatcg cctttagtga ctagagtttg cacaatttct
      481 gtagtaaaat cattcatagg gaagtcctct tttctgtgaa ttggttgtcg ttaactttat
      541 tctacagaag ggacttcctt ttttgtatgg attttttcat ttacacaaaa tattttatac
      601 tctctggttt ttcacatttt tacataaatt tgtcttaata tcgtagatgt aagcttttga
      661 acaccattta ttcggaaaaa gggagctgtg acaagatttt tgtcgcagct ccctttaatt
      721 ctgattaatc tattagatca atcatcttta tcgtctgatt ctttggtctc tggattcgct
      781 ctagcataag ctgaagcggt gttccagtaa tctgtatagt tttcatcagt accgcctacg
      841 ccgaatttaa aggcgctttt cttcggacca ttttttgccc aaagtgactc cactgttttt
      901 cctggcgttt ggatggattt tttattgaca gtaacttttc cagctgtttg tcctgtgaac
      961 ccagatactt ttttcttcat cacatcggat gataagtcaa atttctcatc agtgccgaaa
     1021 atctcagggt ttgcttggta agcttgattg attagattgg caagataatt agccgaacgt
     1081 attcttgctt ggctgtccat tccagtattg tcatcgtgtc ctgcccaact actgatagtg
     1141 atagatggag ttgagacgat cagccatgaa tctcggtatt cattggtaga accagttttt
     1201 ccgacccaat ctgcatttcc tagatttccg tttaagcttg agatgacatc tttgaatggt
     1261 gtagtgattt tttcattgat cacactacgc atcatgtcat tcatgatcga tgccgttgct
     1321 tcggaataaa tacggacaga attactttca tgtttataaa tcacattacc tgcattatct
     1381 gtgatggaat caatgatata gccttcttcg tagactccac catttgctaa ggcctggaat
     1441 ccatttgtct gttgaagcgt cgttacatct actgtcccta aaggagccga ttcaacgccc
     1501 caattatcat ttgctgggta gttcatcttc gccaagtagt gctcgtaagc gtattgtttc
     1561 gatccaccgg aattcaacac atcttgatat aagtgataag ctgggatatt gttggaccat
     1621 tccagtgact cacggacggt ttggaaggtg ttcgaccctt cattggtcgc attgacgatt
     1681 ttctcaccag cattttttcc ttcttgccaa gtcgttgcat aatctgatac acgggattct
     1741 gatccgacaa gtcccatatc gattgcagga ccgtaaacaa gaaccggttt gatggatgaa
     1801 ccagcttgac gttctgtatc aaaggcgtgg ttattttgat tctggctgta attccgacca
     1861 cccacgaagc catagatacg tccggttctg ttatccatca atacatttcc agtctccact
     1921 tggctaccag caccatcatc taacatgtag ccataatttt ctacgccggt ttgtaatgca
     1981 gcatagatat ctttatcgat cgtcgaatgg atcgtatatc ctttgttttg gatcgtttgc
     2041 tgtgcttttt gataataagc ttcactgacc gatgaatctg acatatcagc attatctttt
     2101 tctgccaatt gttgtgcaat aatccttgtt gcttcgttca tcacggtata atacaagaat
     2161 tctctgtcag tctgttccgc gatttgttgt gggagaaagt cttttgtaag atcataggct
     2221 ttcgcttctt cgtattgttc tttcgtaatt tgtccttcac ggtacatact gaataaaaca
     2281 aaatcttttc tggcaagacc ggcagaaaga tcttctttta gcgcgcctgt attcgtgtat
     2341 ggactatacg tgatagggct ttgaggcaaa ccagcgatat aagcagcttg tggcagagtc
     2401 acatcctttg catctacacc aaagattcct ctagcagctt cctggactcc agcaatattt
     2461 tgccccttat tattccgacc aaatggtgag acattcaaat acgtagtaat gatctcattt
     2521 tttgaaaagt gtttttctac ttgtgcagaa agaacgatct cgtttgcttt acgtttgaat
     2581 gttgtttcat cggttaggat ctgttgtttg actaattgct gtgtcaaagt ggatcctcca
     2641 gacgagccga tgccagtcgc ttctgaaact agtgctcgta aaacagcttt agggacataa
     2701 ccttgatgtt tatcgaagta ttcatcttct gtactgataa tcgcggtttt caataaaggc
     2761 gaaatcttat cagatgaaat agtcgtccgc atcagatctg tctggatttt tgatatctct
     2821 gtattatctg cataaacaat ctttgacgtt tccgtaatgt ttcctagttc tgtctgcagt
     2881 tcttttttag tcggcaattg tgtgtcttcg acaagatagg caaagtagcc agcacctatt
     2941 ccgataccta gagctgcgaa caacaaaaga agaacagtaa taaaaacagt caatgattga
     3001 aacactctaa aaataatctt aggaacaaac catttttctt tttttcttct attctgtttt
     3061 tttcgagtat ttttgcgtgg caaaagggtc acctcaacta atggttttat ttcgctaatt
     3121 atagcaaata ataggacaaa aaaacagaca aggctgtttt tgacaggaat ttctttttcg
     3181 aatgagataa aacgtgagga aatgatcttt tttatgaaag agttcttaag cttttcttta
     3241 gggaaagttt cagttactat ggagaacagg gcaaagaaaa acaaaccatt ttctttgtac
     3301 atcatcttat caatatacag gaattgttcg aaacaattgc ctttcaagga ggaatccatc
     3361 catgttatta ggctcacatg tcagcatgag cggaaagaaa atgctgttag gcgcggcaga
     3421 agaagcagcc agttacggat cttcgacctt tatgatctat acaggcgctc ctcaaaatac
     3481 tagaagaaaa gctatcgaag agatgaatat tgatgccgga aaagcattta tggcagaaaa
     3541 cggactaaca aatatcgtcg ttcatgcgcc ttatatcatt aatttgggaa atacgatcaa
     3601 accagaaatg tttccatttg ctgtacaatt tctgagagaa gagattgtcc gtgcggaagc
     3661 tttaggtgct actcaaatca cccttcaccc aggcgctcat gtaggagcag gggcagaagc
     3721 tgggattgct agaattattg aggggttgaa tgaagttttg acaaaagaac agacggcaca
     3781 gatcgctttg gagacgatgg cagggaaagg tacggaaatc ggtcgaacat ttgaggaatt
     3841 agcgcaaatc atcgatggag taaaattgaa tgaaaaatta tccgttactt ttgatacatg
     3901 tcatacgaat gatgccggat ataatataaa agaagatttt tcaggtgtaa tggaagagtt
     3961 tgataaggtc atcggattag atcggcttaa agtactgcat atcaatgatt ctaaaaatcc
     4021 acaaggttct cataaagatc ggcatgccaa tatcggtttt ggaacaattg gatttgacac
     4081 attgaacaaa atcgtccatg atccaatgtt tgacaacatt tcgaaaatat tagaaacccc
     4141 atatgtcggt aaagataaaa agaaagctta cgcaccatat ggaaaagaaa tttccatgtt
     4201 caaagcgcaa gcattcgatc ccgaagtgtt ccctgagtta ttagcagaag aagtcacact
     4261 ttaagttttc tacctgacag caacaaaaaa ggattgggca gtctgccttg tccttttttg
     4321 tttaagaaac aaaggagtgc tttttgcatt cgttttcgcc atgctccttt tgccccctct
     4381 ttttaaattc agacaaacag ggtatactga tagaaaagta aaaaacgaaa agtttcacaa
     4441 acaagtagca aaacgtgatt gaaaggacga aaagatgctg aacattttaa accccttgat
     4501 cgaccaacta aaaaagaaac atacatactt tattcttggc atagccgtat tatttacaat
     4561 tattcggttg tatttatata gtaaagcaat ctatggaaca gatatgtcgg cgacctatga
     4621 tgatcagctg ctgattcact atgctcaaaa tattttggat ggtaaatggc taggggagta
     4681 tacgactaca acactttcca aaggaatctc ctacagctta tttttagtac ttgcacataa
     4741 actgcatctt tcttatgcag ttttgttcgg catgttcagt atttttgcca gtatattggt
     4801 tgcactcgcg tttaaaccga ttgtgaagaa caagtatctt cttttaagta tctattttta
     4861 ttttttgttt tctccgatta gttttacaca tgaatattcc actcgaacat atcgtaatgc
     4921 cctcgtgatt ccggctgttg agatcattat cgcttgttta ttagctattt attaccgcaa
     4981 atacggttct agcagaaaat tgctgccatg gtttttaggg ctttctgtcg cttttccttt
     5041 cttttggttc attcgcgaag attctatctg gcttcttcct tttactgtag tagctttggg
     5101 tatcagtttc gtccagatct tgctagggca agacttttct ttcaaagaag taaagaagtt
     5161 aaaaaaacga ctctttcttc tctcgaaacg ggaatggctg aaactttcat tgtttttact
     5221 tcctatagtt ggcttttcca tcgtctatca gtggatcgaa tataaaaatg agacaacata
     5281 cggtattgca accatcaatg accgttccag tggagaattt ggacgtttga tgaaacaact
     5341 gatccgaata gatgatgaaa cgaacttgaa taaagaaaac agtgagatat gggtctcaca
     5401 tgctgcacta aataaagcat tagccgcatc tcctacattc catactgttt ctacccgtat
     5461 tgacgaatta tatacgactc acgcgtggac tcaaggtgga aaagtaaaag aattacccgg
     5521 agatatcatc ttttgggcat tgcgagatgt ggtaagcgaa tctggttatt accaaaacaa
     5581 tgcaaaagaa accaatgatt tttggaaaaa agtcaaccaa gaattagaag aagcctatca
     5641 caagggaacc atcaaaaaga aaaaagagtt ctatctgatg ggatcaggag atggaaagac
     5701 gatgagtgat ctgccaatcg tattcgattt ccttcaatca ggatacgctt atgcatggcg
     5761 atataaagat ttccatcaag gaggaaatta tagtttcgga acgcaagaac aagtcaaaca
     5821 ggctcaagaa ttattaaacc ttccgctaat ggagaattgg atcgatagta atgaaccgag
     5881 aacacctaat ttccaattaa caaaatcagc aaaagtcgcg aatgtcatca taaaaattta
     5941 ccaagttctg gctattcccg tgctgattgc ttcaatagca gggatattac ttatgatact
     6001 aggaaccgta tttgcaaaag ctgatcgtgc ctattatagt tcctctttga ttgtggtcac
     6061 cggattaata ctgacacaat tggtttttct tttcggtgtc tcatggtttt gttcttatgc
     6121 gccagaaaaa aaggactatt ttttaagtgt ctacactggt gcaggcgtgc cattaacgca
     6181 attcatcgta gtaacaagca tgctagtttt cagtaaatta aatctgaaaa aagctttatc
     6241 ttctaaataa aaagaccctt ggtagctaga tttttaacct aactatcaag ggtctttgtg
     6301 tggaaaaatc agtttttatt cgatttcttc taccgtttcc tgcaaaagct gttcatattt
     6361 tttgtttcct ttatataaat caccaatcgt ccatgccaac aggagaagga caagaacaat
     6421 gattacataa gcgatgtgat cagccaatgc tagttgagcg gaagtggctt gctcgccgaa
     6481 gaaactttcg atctgtcctg gtaatcctcg taagttcaag taagttagac taatcacaga
     6541 gaaccagcca agaagtttta cgatgaatga atttttaaat cggctaccca tctccgcttg
     6601 gctgttggtc atcatcagta aaggaatcat cgaaaatggc aacgcaaaag ccaagaacac
     6661 ctgggattca ttcattaagt tattcaaagc ggcatgttct tcgatcgttc cttttgtact
     6721 tgtaaacaaa acacaaatca atacaggaat cacagaaagc aatcgtgtga ccagtcttct
     6781 tagccaaatc gggatacgca tatggatgaa tccttccatg atgacttgac cggtcaacgt
     6841 accagtaata gtagagttct ggccagaagc aagaagtgct acagcaaata aagtggacaa
     6901 gataccagat tttgctacag caatcaaaat accattgctc atagctgatg gatcagataa
     6961 agcttcatac agaccaaaaa aggaaggatc ttggacagaa ccggttttga agacagcgac
     7021 acccatgatc aatagcagtg cattgactag aaaagccatt gacaactgga tattagaatc
     7081 ccaagtagaa aaacgtacag cttgtgctac atcttcttcg ttttggtgat cgatcttccg
     7141 agtttgtgaa actgctgaat gaagatataa gttatgcggc atgactgttg caccaataat
     7201 ccctaaagct ccagtcagcg gagtttcgcc gccgatgacc ggtgagtctg agaatgtatg
     7261 actgttaggg ataaagccag caaataaact gtgccaatct gggttagaca agccaacttg
     7321 atagacaaaa acgaataaaa tgacgaaaat taagcagaca accagtgctt cgatcttacg
     7381 aaacccgact tttgtcagta gtaagagaac gaaaacatct aatacagtaa taaagacggc
     7441 aatcaccaat ggaatatcaa aaagtaagta gagggcgatt gctgcaccaa tgacttctgc
     7501 gatgtctgtt gccataatgg ctaattcagt caagatccat aaaatgacac ccaatgtctt
     7561 acttgtccgt gctcggattg cttgggccaa atccatttgg gtcacgattc ctaattttgc
     7621 tgccatatac tgtaaaagca tagcaattag actagacatc aaaataactg acatcaataa
     7681 atattggaaa ttttgtccac cagtgatcga agtggaccag ttacctggat ccatataacc
     7741 aactgctact aatgcacctg gtccagagta agcaagaagt gttttaaaaa aactttttcc
     7801 ctttggtaca tccactgttc catttacttc agctaaagat aaaccatttg tatgttcaat
     7861 aagtcgatgc ttatgaaatt gtttgttgga ttgattattc aagaaaaaac cacctttcct
     7921 atttatctac gattacattc tacacctcag aaagaaaaaa ttaaagaaaa agattcggta
     7981 tgcctaattt tttttcgtgg tgtgtcttgc tggttaatga aagaaatttt gttcttctct
     8041 aaaaaaacca ctattttttg catgaaacta aagaatacgc ctttcttttt tcaataaact
     8101 ttagtaaaat aaatcattga gagcaattcg gaaatagaaa ggagagattt atatggtatc
     8161 aacaggtatt gtcgtactta ttgctattat tgctttgcta atcggcgcag taggtggctt
     8221 tttcctagcg cgtaaatata tgaaagacta tttagaaaaa aatcctcccg ttaatgagga
     8281 catgttgcga tcaatgatga tgtcaatggg gcaaaaacca tctgagaaga aaattcgcca
     8341 gatgatgcag caaatgaaga accaagggaa aaaataggtt ctgacccaaa gactttttgc
     8401 gggctgattt tttcagtccg ttttttattt cgtaatttgt tagactattc agactattca
     8461 gcaaaaaaga caagcacaga caatcctatg ctcttgataa agacaaagga ggagtaaaat
     8521 gtcgattttt agaaaattag gttggttttt tcgtcaggaa aagaaacatt atttaattgg
     8581 gattttctcc ctattcgcag tggccttggt acagttagtt cctccaaaag tgattgggat
     8641 catcattgat gagatcgccg ataagaattt atcaataaag atcatttcct ggtggatctt
     8701 attattgatc cttgcagctt tcttacaata tctttttcgt tatatctggc ggacgaatat
     8761 ttggggaagt gcagctcggt tggagagaga acttcggcag cagctattca aacattttac
     8821 agaaatggac aatctctttt accaaaaata tcggactgga gatttgatgg ctcatgcaac
     8881 gaatgatttg aatgcaatcc agaatgtagc aggagctgga atcttgacct ttgcagattc
     8941 agtgattacg ggcggaacaa cgatcattgc gatgattttg tttattgatt ggcgcttaac
     9001 actgattgcg ctgattcctc tgcctttgct agccgttact tcacgagtgc tggggtcaaa
     9061 actccacgat gcttttcgtg attcgcaaga agctttttct agtatcaatg ataagacaca
     9121 agaaagtatc actgggatca aagtcattaa aacatttgga caagaaaaag aagatctcgc
     9181 ggattttgaa gaaaagattg aagatgcaat tattaaaaac aaacgagtca attttttaga
     9241 ctccttattt gatccattca ttaccttgat tatcggtctg tcttatgttg caacaattat
     9301 tttaggtgga aagtatgtct tggaaggcac gatctcaatc gggcagatgg tctcatttat
     9361 tagctatatc ggtatgcttg tttggccgat gtttgcgatt ggtcgattgt tcaacgtatt
     9421 ggaaagagga aatgcttctt atgatcgcgt caatgaactt ttgcatgaaa agacgcatat
     9481 cgtggaacaa ctgggtgcag tgcagacccc ggcaaaaggc gagctgaatg tagctatttc
     9541 ctcttttaac tatccgaaag aagaatcacc agcattagaa caaatcaaat ttgcgatcca
     9601 agaaggagaa acattaggta ttgttgggaa aactggtgcg ggaaaaacga caatcatcaa
     9661 attactgatg cgtgaatacg atgattatca agggaaaatc caatttggcg gacgttcgat
     9721 caaagaatat tcattgaatg ctttattagg atcaatcggt tatgtgcctc aggatcactt
     9781 cctgttttcg atgaccattc gagataatat ccgtttttct aatccaaaac tttcagaaga
     9841 agaagtagag catgcggctt ctttagcatt tatcaacaat gaaatcaagg aattgccaaa
     9901 aggttatgat accttagttg gagaacgtgg agtctcttta tctggcggac agaaacagag
     9961 gatttcgatt gctcgagcat tgatcaaaaa tcctgaattg ctgatcttag atgatgcact
    10021 ttctgcagta gatgccaaaa cagaagaagc tatcttgcat aatttaaaag aaataagaaa
    10081 agagaaaaca accatcattg cagctcaccg tttaagcagc gtgatgcatg caaaagaaat
    10141 cattgttgtc gatcaaggga aaatcattga acgaggaacc caccacgagc tgcttgcatt
    10201 aggtggctgg tatgaacgaa tgtgggaaaa acagcaatta gaagcgaaga tagaaggagg
    10261 cgaggcctga tggatgaaaa ttatcaatcg gaatggacaa aatcgattcc acttaaagaa
    10321 cagaagaaga tcatcaaacg actgatgaag ttcgccaaac cttttagaag aacgttcatc
    10381 gttgctatct tatttgcgtt tgccctgtct gtgatcaatg tcctattgcc aaggatcatc
    10441 caaactttta tggatgatca tctggctaaa cagtcagcta cgacacaagt tattttgttt
    10501 tttgctggtg tgtacttgtt tggagtcata gtcaaaagta tcatttggtt tttccaatgg
    10561 tatttgtatt ccatggcttc attgaagacc tatcagtatg ttcgggtaag attatttgaa
    10621 aagctgcata cactaggtat gcgttatttt gaccagacac cggcaggatc gaccgtttct
    10681 cgagtgacga atgacacaga aacgctattt gaattttggt atgtcttttt gatggtattg
    10741 actgggatct ttgcagtcgt ttcttctttc gttgccatgt ttcaaatcaa tggagaaatt
    10801 gccttgtgga ctttggtgtt cttgccgatt ttaggaatcg tcatctggta ttaccagaaa
    10861 ttcagttctc gtatttaccg aaatatgcgg gaaaaattaa gtcagctgaa cacgaaattg
    10921 aatgaatcga tttccggcat gcgtattatc cagcagtttc gccaagaagc tcgtttagct
    10981 aaagaatttg aacaagtgaa caacgagtat ttagacacgc gctatgcgat gatcaaaacg
    11041 aattcgattc tgttaagtcc aatcatcaat tttctctatg cattggcgat tgcgttggcg
    11101 ttgactttgt ttgggatcga tgcgttgcat tctcccgtag aagttggact gatctatgca
    11161 tttacgacct atgtccagga attttttaat ccaatgtctc aaatgatgga ctttttgagc
    11221 acctttacag atgggatggt tgccggtagt cgtattttga aaatcatgga tacagaagaa
    11281 tatgcaccgc aacaaaatcc tggagcagat caagcgatta cttcaggtaa gatcgagttt
    11341 cgcaatgtaa gcttttctta tgatggagag cacaatgtat tgaacaatat ctcttttgtc
    11401 gctaatccgg gtgagaccgt tgcattagtg gggcacaccg gaagcggaaa aagctcgatc
    11461 atcaacgtat tgatgcggtt ttacgaattt tatgaaggcc aaatcttgat tgatgatcat
    11521 gacattcgag aatatccaat ggaagagtta cgtaagaaaa tgggattggt cttgcaagat
    11581 gccttcttgt tttatggaga tattgcagga aatatccgga tgatgaatcc agctatcaca
    11641 gacgaacaga ttcgccatgc agctgagttt gttcaagcaa acaagttcat cgatgaatta
    11701 ccaaaaggat atcactcaaa agtgatcgaa cacggtgcca gctattctag tggacaacga
    11761 cagctgatca cttttgcgcg aacaatcgtg acagatccta aaatactagt attggatgaa
    11821 gcaacagcaa atatcgatac tgaaaccgaa ggattgatcc aagaaggatt ggcaaaaatg
    11881 cgacaaggac ggacaacgat agcgattgcc catcggctgt ccacgattcg ggatgcagaa
    11941 ttgattttag ttttagacaa aggtcatata gtagagcgtg ggaatcatga aacacttcta
    12001 gcgcaaaatg gtttgtatgc agatatgtac aaactgcaaa gcagtggtgt gtaaaaagag
    12061 gttgtgacag tagcatagta gcattttgcc ctaagcgcta gagtgaaaat aaaacagagc
    12121 ttcgcagatt cttcatctgt gagcgttagt ggcgtagtca cccgatttaa aaaagacgac
    12181 gacagctagt caaaagcggc taataaccac taagaaagcg ttgacaaaag cgtttgcttt
    12241 cgataatata aagaaaacga tacaacttgg atagtgattg cctgatgcaa gctaaacctg
    12301 atgagtctcc catggttcca tggacggctt tttgggtttt tctttttgtt ttggatacag
    12361 ccctatatat catggaaagg agcaaaacat gctgattgag aaagtactga ataacaacgt
    12421 cgtaataacc agggatgaaa gaaatcagga aatgatcgtg atgggaaaag gtttggcatt
    12481 caaaaagaaa gtgggtgacc cgatttttga ctcatgcatc gacaaaaaat ttcagttgac
    12541 aaatgacggg ctttcacaac agttccaaga attggcacaa gcgattcctc ttgagtactt
    12601 agaaatttct tgtgaaatca ttgaatatac aaaagaggct ctacaagtcc agttgaatga
    12661 ttctgtgtat attgcattga cggatcattt gtatacggct atcgaacgtc acaaaatggg
    12721 aatatttgta ccaaatgtat tgctgtatga tatcaaacaa ctatttccta aagagtatgc
    12781 catcgggaaa aaaaacagtg aagaaaatct cggataaata ccacatcaag ttatcagaag
    12841 atgaagccgg atttatcgca ttgcatttag tcaatgcaca aggcattcaa agccaacaag
    12901 acaataccta cctgttcact ataacaatac aggatattct aaatatcgtc cgctattact
    12961 ataagattac atttgatgaa gattcagtcc atttttttcg attcgtcaca cacttacgtt
    13021 tttttctgac acgcgtccta gatggagaaa aatcagaagt gggagaagtt tctgaggaat
    13081 tgtttcaggt tattcaaaaa aaatataagg atacgtttgg ttgcgttgag aaaatagcag
    13141 actttttagc cgagacgttc aactaccaga tgtccacgga cgaaaaaatg tatttaacga
    13201 tccatactgc gcgtttagtg catgagaatc gttgtaaata aataaaaaaa ggaggataaa
    13261 ttaacaagaa cgtatgaaat aagggtagtg acattcagtt ggctaaacct tccactaata
    13321 aattggaggg aatagaaatg ggaaaatacc aagaattagc gagaaaaatc gtagaaaatg
    13381 tcggcgggaa ggaaaatatc aatggtttga cacattgtat cacaaggctt cgtttcaaat
    13441 taaaaaacga agaaaaagca aatgatgaga tcttgaaaaa tatggacggt attgtaacgg
    13501 taatgcgagc gggagggcag tatcaagttg tcatcgggaa tcatgtgcca gtcgtgtttg
    13561 aagaggttat aaaagcagga aaccttactt ttgatgaggc tgtttcaaca gaaaaaatga
    13621 gaccgtttga tatgcttatt gatggaatat cgggctgttt ccaaccattc cttgctatcc
    13681 tagctgctga cgggatgatt cgtggactga cagcattcct tgtcttctta ggcgcgtttg
    13741 accgcggttc tggcacattt gttatgtttg ataatatcgg tgattctgta ttccaattta
    13801 tgcctgttat tatcggttta acagctgccc gtaaatttaa agtaaatgaa ttcgtaggga
    13861 tgttgcttgg cgcagcatta atgaatccta gcttgtcgct ggaagctttg agtggtgcag
    13921 cagaagcacc actaacgacc atattttctg gcactatttt tgaagcacct atttatcaaa
    13981 cagtattcgg cattccctgg attgcgcgta actacgcttc ttctgtcatt cccattatct
    14041 ttattgtgtt gttagctagc caaattcaga aaccaattaa aaagttagta cctgagatga
    14101 tcgcgaattt cttcgttcca ttcttcaccg ttcttattac aatgccttta ggttttttac
    14161 tcgttggacc agtattcaca ttcgcaactg atattttaat ggctggattt gaaacacttt
    14221 tagcactttc gccagttatt tatggagcaa tcgtaggctt cttctggcaa attctggtga
    14281 tgtttggtct ccattgggca atcgtaccaa tgggattgat gcaattttct gtgaatggtt
    14341 ggcaaaatat tatgacacct gttgcagtag tgtcttttgg acaaactgca gcacttaccg
    14401 cactctactt taagcttaga aatccaaaag acaaagcaat tgcaatacca gccattgttt
    14461 ctggtattgt ggggattaca gagccagcta tttatgggtt tacattgcca cgtaaaaaaa
    14521 tatttatttt tacctgtgtc ggtggtgcgt tagggggcgc gtatagtggg ttaatgaatt
    14581 taacttcttg gaatcaaggt ggactaggca tctttacaat tcccaactat attagaccag
    14641 atggtgattt aacagatgtg attaatgtat tgattgggat cgccattgcg atggtggtat
    14701 cctttgcgct gacttttttc ttttggaagg aggaagcaga tgaaacagat gatagacaga
    14761 aaagatcagg taaagaaatt gtaaaaacac cgatacaagg acaaatcgct ccactgaatg
    14821 cagcaaagga tgcagctttt gcacaaggaa cactaggtcg aggaatcttg atctaccctg
    14881 aaaaaggaga agtacgtgcc ccttttgacg gaacagtcat gtccctgttt ccgacgaaac
    14941 atgcaatcgg gatggtttct gaagcaggtc tggaattgct gattcatgtc ggattggata
    15001 ctgttcagtt ggaagggaaa tattttgaaa gcttggtgca gcaaggagaa agagtgaaac
    15061 aaggcgatct gttgcttcgc ttcgacaaaa agaaaatcga agaggaagga tacagtttag
    15121 aaacacctgt gattgtcatc aattacatgg attatttgga tattttggaa aatcaagggg
    15181 aaacggtcgg gccagacgat gcacttatca ccgcactgac ctgaacggtt aggtgaaaga
    15241 atggactagg cttaactatt ctttttagtc cacttcttta ttttcaaaat tgaaaggctc
    15301 tttcatgatg gctgagctga tagggcgttt atcaaatttt tgtcctacag aaattagatg
    15361 gactaaattc tgttgataat tttctgcaaa aacacaacta tataacacgt ctagaagata
    15421 acaaatcgat gtattgatcg taaaattacc aattttcgaa tacagtcgtt cgcgtgtcgt
    15481 aagtcgcaaa gcggctgtac tcattgaaga tagcgttgaa tcaccgatac tggtcaaagc
    15541 taaaattggc acatgatttt gtttcaatat ttctgcggta cgaaggatca cattgttttc
    15601 acccgtataa gaaatcagaa tcccacacat atcacttgtg caattgtacg cttcatagaa
    15661 cgattcacca aaagtggtga caatcgtgac atccattttg atgcgcttca ttttcagcat
    15721 gaaatcttga gaaatcaaag tattcgagtt actggcgaaa atcacgattt tttttgcttg
    15781 aagcattagt tgtttggctt tttgcagttg gtcatgatga atgagagaaa gtgtatcttg
    15841 aatcgtcatc tgttctaaag aagccatttt atttgcgatc gtcataatac cgtctgtggt
    15901 ggaaaaagga agattcgcat ctacgtcttg aaaattccct tcaagatagg ctgtttcagc
    15961 taaaaaagca ttacgaaaac tgacccagcc atcaaaaccc attttttttg ctacacggat
    16021 gagtgtggac gggtggacat aacatcgctc agcaatctct tggatggtca tttcgccaag
    16081 tgctggagaa tcgcttaaga gaaactgaat cacttcattt tctgaaggag aaaaggagat
    16141 ttctttgatt ttttcacgta ataacatgta gataccttct tcttgttttt ttacgtaaat
    16201 cataactttt ttgaaaatga tttgtaaacg gatttatgtt atatagctta ataaagacat
    16261 tttttgacaa gatatttaca aaaatccaga agaaaacact aacttttgta aatgttactg
    16321 gaaagaaacg aatttctatt gtttgttttg tttacaaaaa agataataaa agtgtaaaaa
    16381 gtaaaggagt gagaacatgg caaaaggaat caaaatcgct acgattggtg gaggcagcag
    16441 ttacaccccc gagttgatgg aaggatttat caaaagatat gaggaattac cgatacgaga
    16501 aatctggctc gtagatgtag aagaaggaaa agaaaagctt gagatcgtca gtcagatgtc
    16561 tcagcgtatg tgggatgctt ctccgtatga agtcaaaatc tatgcaactc tggatcggga
    16621 agaagcattg aaagatgcgg attttgttac gactcagttt cgtgtcggtc aattagatgc
    16681 acgaatcaaa gacgagcgga tccctagtta ttatgggatg attgggcaag aaacaaatgg
    16741 tgctggcggg atattcaaag cttttcgtac cgttccagtg gttttggcaa tcgttgaaga
    16801 catgaagcgt ttatgtccaa atgcttggct gattaatttc gcgaacccaa gcgggatggt
    16861 gacagaagca gttatcagat atggcaaatg ggataaagtg atcggattat gcaatgtgcc
    16921 agtaatggcg atgatgacag aaccaacaat gcttggcaag aaaaaagaag agctgatcta
    16981 caagtttgcc ggtctaaatc acttccattg gcacaaagta gcagataata aaggaaatga
    17041 ccgcacggca gaattgattg atcgtttata cgatgaagac aatggattgc cgaaaaatat
    17101 ctttgacatc ccgtttttca aagaacaatt aaaacaaatg aacatgatcc cttgtggcta
    17161 ccaccgctat tactatcgtg aagaagaaat gctggaacat gctttagaag aatatcaaac
    17221 gattggtaca agagcgcagc aagtgaaaga aacagaagca gagctgtttg aattatacaa
    17281 agatccagaa ttggatcata aacctgaaca attgcaaaaa cgaggtggtg cttattattc
    17341 agatgctgcc tgtgaaacga ttgcttcgat ctatggaaat aaagaaacac agatcgtggt
    17401 atctacaaag aatgatggag ctgttcctga tctgccggca gattgtgtag tagaagtcac
    17461 tgcctatgtt ggcgcacaag gcgcacgaaa tgtagcgttt ggttcccttc ctgctgctga
    17521 aagaggctgg ttgcaagtaa tgaagaatat ggaattgctg acgatcgaag cagccgtgac
    17581 tggtgattat ggaacagctt tgcaagcgtt tacgatcaat ccgcttatac gatcaggaaa
    17641 gacagcaaaa cggatcatgg atgagttgtt tattgcacat cagcagtatt taccgcaatt
    17701 caaggaaaca atcagccggt taaaagcaga aggaatcgaa gtccaagatg aactggcaag
    17761 agaattatca agcaatacac atgtaatgga ggaagtatga tgggattcag aaaagatttt
    17821 ttatggggcg gagctacagc agccaatcaa tgcgagggcg gttatgatga aggcggacgt
    17881 gggttagcga acgtagatgt tgtgccaatc ggagaagacc gttttcctgt cattactgga
    17941 aaaaagaaaa tgtttgaatt tgatgaaaat catttttatc cagcaaaaat tgcgatcgat
    18001 atgtatcatc attataagga agatattgct ttatttgcag aaatggggtt caagacttat
    18061 cggatgtcca ttgcttggag tcgtattttt ccaatgggcg atgaaaaaga gccaaatgaa
    18121 tctgggttga aattttatga ggatgtcttt aaagaatgtc aaaagtacgg gatcgagccg
    18181 cttgtgacga ttacacattt tgattgtccg atgcatttga tcaaagaata tggtgcgtgg
    18241 cgttccagag aactcgttgg tttttatgaa aatctgtgcc gagtcatctt caatcgctat
    18301 aaaggacttg tcaaatattg gttgacattc aatgagatca atatgatcct tcatgcacca
    18361 ttcatgggcg cagggctgta ttttgaagaa ggagaaaatg aagaacaaat caaatatcaa
    18421 gcggcacatc atgagttggt tgcaagtgcc atcgcaacga agatcgctca tgaagtagat
    18481 ccggagaaca aagtaggctg tatgctggca gcaggagcat attatccata cacttgcaat
    18541 cctgaagatg tttgggcttc tagaaagtca gatcgagaaa attatttctt cattgatgtc
    18601 cagtcacgag gagaatatcc cgcttatgca ttgaaagaaa tggaacgaaa tggcattcag
    18661 atcaaaatgg aacctgggga tgccgcattg ctgaaagaac atactgttga ttttatctcc
    18721 ttttcttatt attcttctcg agtttcgact actgacaacg aactcttgga acagactgca
    18781 ggaaatatct ttgcttctgt caaaaatcca tatctggaag caagtgaatg gggttggcaa
    18841 attgatccat taggcttgag aataacgatg aatgatattt atgatcgtta tcaaaaacca
    18901 ctatttattg tagaaaacgg cttaggtgca gtcgatacgc ccgatgagaa tggttatgtg
    18961 gaagatgatt atcgaatcga ctatctggca gcacatatcc aggcaatgaa agatgcagta
    19021 gaattagatg gagtcgatct attaggctat acgacttggg gatgtattga tttagtatca
    19081 gctggtacag gtgagatgaa gaaaaggtac ggattcattt atgtcgatcg tgacaacgaa
    19141 ggaaatggca cattagcccg ttcgaagaag aaatcattca actggtataa aaaagtaatt
    19201 gattcaaatg gagaaaatct ttcgaacgat tgatcaatga tttataaaag tctattgttt
    19261 ttcagcggat ttttcgagta taataaagct attcgagaaa cgcaagcaga acaatagact
    19321 ttttttcgca ttggctgtaa agtatgctta cttgattaag taaaggagaa tctacatatg
    19381 tatgatgtaa tcgttattgg cgcagggcct gctgggatga ctgctgcttt atatgcttca
    19441 cgttcaaact tatccgtatt gatgatcgaa caaggcgctc ctggcggcca aatgaacaat
    19501 acggcagaaa tcgaaaacta tcctggtttc gattcaatca tgggtcctga tctagcttat
    19561 aagatgtatg aaggtgtttc aagatttggg acagaaaatg cttatggaat cgtgcaagat
    19621 atcaaggatc acggcagcca caaagaagtt atttgtgaag ataaaactta cgaaggaaaa
    19681 acagtcatta tcgcaacagg ctgtatccac cgtaaattag gcgtacctgg cgaggaagaa
    19741 tttgctggac gtggcgtttc ttactgtgca gtttgtgatg gtgcattttt ccgtaacaaa
    19801 cgtctagtag tgatcggagg aggagactca gcagtagaag aagccatcta tttgacacaa
    19861 tttgcttctg aagtagtcat cgtccaccgt agagatgaat tacgtgcaca aaagatcatc
    19921 caagaccgtg catttgcgaa tgaaaaaatt tcctttatgt gggatacagt cgttgacgaa
    19981 atcgttggaa atgaaatggt agtgacaggt gtgaaagcac gtaatgtcaa aactggcaat
    20041 gagtttgatt tggcagcaga cggagtattc atctatgtag gattggatcc attaactgca
    20101 ccatttagag aagctgggtt gacgaacgaa gccggctgga ttccaacaga caacgaaatg
    20161 aagacgaata taccaggcgt ttttgccatc ggagatgttc gtgaaaaaga tttgcgacaa
    20221 atcactactg ctgttggtga tggtgggatt gctggacaac aagtgtataa atacttggaa
    20281 tctttagaag aaacaactgc ataagtttaa aaagagtgaa agaacggttt caattgttct
    20341 tttactcttt tttttgcgtg aaatctactg aatccgattt atgcgaatac ttgtaaccgt
    20401 gcttgataaa agaatctttt cttgatttaa gcgttttttg gtcctcatac tttcatatct
    20461 aataagtgaa tgctatactt attaagaacc atatttcaga aagaggtgtt attttcatgt
    20521 cttgggaaca agtgtatgaa caatgggtac atgaagaaaa tttagaagaa aacttaaaaa
    20581 aacaattaaa tgatctgggt gaagatcctg aaaaacttga agatgctttt tatgcaccgt
    20641 tggaattcgg tactgcaggc atgcggggaa tcttaggacc tgggatcaat cgaatgaaca
    20701 tctatacggt aagacaagca acagaaggac ttgcacgttt catggataca caaggtgaag
    20761 aaacaaaacg ccgaggtgtc gctatcgctt atgactcacg ccatcaatca ccagaatttg
    20821 ccatggaagc agccaaaaca ttagcacatc acaatatccc ttcttatgta ttcgaaagtt
    20881 tacgtccaac accagagctt tcttttgcag tccgtcattt gaagacgttt gcgggaatca
    20941 tggtaactgc atcccacaat ccagcagctt acaacggata taaagtctac ggagaagatg
    21001 ggggacaaat gcctcctgca gacgcagatg cattgacgaa atatgtccgt gaagtcagca
    21061 atccgttaaa agttgaagta ttatctgacg acgaagctga acatagcgga ttgatcacga
    21121 tcatcggaga agaagtggat gccgcttatc tagaagaaat caaagcagta acgattgatc
    21181 atgaattggt ggaaacaatg ggtaaagatt tgaagcttgt gtacacacca cttcacggaa
    21241 caggaaaaat gttgggcgaa cgtgcgttga aacaagctgg attcgaacaa ttcgtactag
    21301 ttccagaaca agctgtcgca gacccggatt tcactacagt caaatcaccg aatccagaag
    21361 aacactctgc tttcgagtat gcgatgcgac ttggcgagaa agaaggcgct gacttactga
    21421 ttgcaacgga tcctgatgca gaccgtttag gcgcagccgt tcgtttgcca aatggctcct
    21481 atgaagtatt gacaggaaac caaatcggag ccctgtttgc tcaatatatt ttggaagcac
    21541 accaatcgcg aggcacttta cctgaaaatg cagcggtatt gaaatcaatc gtttctagcg
    21601 aattgcctac agcaattgcg gaacactatg gggctaccat gttcaatgta ttgacaggat
    21661 tcaaattcat tgctgaaaaa attcaacaat ttgaagaaga tcattcccat acttttatgt
    21721 tcggattcga agaaagttac ggttatttag ttaaaccttt cgttcgcgac aaagacgcga
    21781 ttcaagcatt gctcttatta gcagaagtag cagcttacta taaaaaacaa ggcaaaacat
    21841 tgtatgacgg cttgcaaacg atttttgaaa catatggcta ctacgcagaa aaaacgattt
    21901 ctgtgacaat gagcggacaa gaaggctctg caaaaatctc tgctttgatg gaaaaattcc
    21961 gtgaagaagc accgacagaa tttgccggag caaaagtcat ccaaacagaa gacttcaaag
    22021 agttgacgag aacagaccgt gaaggaaacg tagaaaaact aacgacaccg ccatctgacg
    22081 tattgaagta tacgctggaa gaaaatggct ggatcgctat ccgtccttca ggaactgagc
    22141 cgaaaatcaa attttatatc ggtgtaaaag gaaactcttc agaagacgca gctgaaaaag
    22201 tagccgctta tgaagcgatt atcaaaaaaa tgacagaaga ataaaaaaac ttgagactga
    22261 tggaaaaagt ggtaaacttt agaagtcagt cttttttcaa gttggaggtg aatggattgc
    22321 cagacacgca aaaaacaaaa gtagagaaag tcagccaact attcaaaatg ttgagtgacc
    22381 ccactcgtct caaaattctg ctgtatctga aagacgggga acaaaatgtg acagcaatca
    22441 cccaagcagt agaaatggaa caatctgctg tatcgcatca actgcgttta ttaagagaaa
    22501 accatgtcgt caagtcccat cgtgagggaa aagcgatttt gtatagtctt gatgattctc
    22561 acgtactgga tattttgaac cagacgctca aacatgtgga acatcaataa cgataggtgc
    22621 aaatagagac aaactggact gtatcaacga atcttcttcg ctgacacagt ccagtttttt
    22681 taagaaaaag acaagaaaaa ctgttagtct gtttcttcgg tggtttcagt tccataataa
    22741 ctatgatctt ctaggtctaa agcattcaaa ataagtgaag ctgtttcttc gatagataaa
    22801 gaagcgacat tgattactac gcagcccaat ttttcataaa gatcattcgc aaaatcgagt
    22861 tcagctcgta ttttttcgat atcggaataa gcagtatcag gatttaatcc gtaagagcgc
    22921 atccgttctt tacggatatt gtttaaaata tctgggttat tcgtcagtcc cacgattttt
    22981 ttaggatcga tctcccaaag ctgtttggga atatgtgctt gtgggatcaa aggtagattg
    23041 gcaaccttta aatttttgtt agctaaaaac aagctcaaag gcgttttgga agttctggat
    23101 acgcctagca aaacgacatc tgcttctaga aacccacgag gatctttccc gtcatcgtat
    23161 tttacagcaa attccattgc tttgatccgt ttgaaatagt tctcattcaa taaatgaagg
    23221 gcgccaggtt gccgaatggg cttgatacca gttaagcgct cgatttctga tatcggcgga
    23281 gtcatcacat caaaactaaa taattgatgt tcttcacaaa aagaacgcgt catctcgatc
    23341 aattcttgat tgatcaatgt gtgtaggaca agggcttttt cttttagtgc atcttgcagt
    23401 gcctgcatca atgttttctc atctgtcaca aatgttctgc ggaataaatt gaactcaacg
    23461 gtaggatatt gggccatagt ggcttgcgct aattttgtcg cagtttctcc agcagagtca
    23521 gagatgacaa aaaaagtaat gatttctgta gaatcggaag acatggataa aacctcactt
    23581 gcattttttt tatgtttatt ataccatact ctagaatgtg tacttcgtaa tcattttgat
    23641 ttggaggaat aagtcatgaa aaaacagaca ttgattgaac aatcactaga aacattgaaa
    23701 aataacggtt ttaaatatac taaaaaaaga gaagcattgt taacttattt agtgaagcgc
    23761 aatcgctacg tatccgctaa agaagttttt gactatatga atcaggaatt caaaggagta
    23821 agttacgata cgatttatcg aaatctccat gattttgcag aaatcgattt actggaagaa
    23881 accgatttaa atggagagat gaagtttcgt tttcattgct gtcaagggga aatcggccat
    23941 caccatcact ttatctgtac ggtgtgcgga acaacgaaag aaatccatat gtgtccaatg
    24001 aattatttcg aagaacagct cggtggatgt acgatcgaag ggcatagatt cgagattttc
    24061 ggaagatgtg aaaattgtca ataaggattt aaataaaata acatttgata attttaaaaa
    24121 aaagcaagat ttccttgacg gtcaaaacag cattagatat aatatttaca ggacagtctt
    24181 gttttttgag atatatttca ataacaatta cggttacttt ttaagctcgg agggagggaa
    24241 ttaacatgtc aaaaacagtc gttcgcaaaa acgaatcact tgacgatgct cttcgtcgct
    24301 tcaaacgttc cgtgtcaaaa gcgggtactt tacaagaatc tcgtaaacgt gaattctacg
    24361 aaaaaccaag tgtaagacgc aagaaaaaat ctgaagcagc tagaaaacgc aaaaaattct
    24421 agttctgtga tgaattggag ttggtagtgt gtcactacta agtagattaa acgatgacat
    24481 gaaaacagcg atgaaagcga aagataagga atctttacaa gtcatccgaa tgatcaagtc
    24541 atctatacaa aacgagcaga ttaaagaagg tcacgacttg acagaagaag aagaattaac
    24601 ggtgttgtct cgcgaaatga aacagcgacg tgattctttg catgagtttg aagaagcagg
    24661 acgtgacgat ttagctgaaa aagtaaaaag tgaaattgtg atagtcgaaa aatacatgcc
    24721 ggaacaactt tccgacgaag aaattcgaca acttgttcaa gaagcaatca cacaaactgg
    24781 tgcttcatca atgaaagaat ttggcaaagt catgggtgcg atcatgccta aagtcaaagg
    24841 aaaagcagat ggaaaccaag taaatgccat tgtgaaagaa ctattacaat cttaatttta
    24901 tcatttaaag gctcttcgtc aatgagtgtt ggtgaagaaa ttc
//
LOCUS       DAZKLM010000035        24862 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3764
            SAMEA115706913-rid22582563.denovo.035, whole genome shotgun
            sequence.
ACCESSION   DAZKLM010000035 DAZKLM010000000
VERSION     DAZKLM010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706913
            Sequence Read Archive: ERR13196647
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24862)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24862)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287823.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287823.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,004
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,849
            CDSs (with protein)               :: 2,849
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 41 of 96
            Pseudo Genes (incomplete)         :: 52 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 15 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24862
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706913-rid22582563.denovo.035"
                     /isolate="VRE3764"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(104..1336)
                     /locus_tag="ACG842_001314"
     CDS             complement(104..1336)
                     /locus_tag="ACG842_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HFQ0247867.1"
                     /translation="MLSDFNELLKKYARLIAETGVATEKGHTVVLQISVEQAPLARLI
                     TQEAYKLGAAEVIVQWTDDQIQREFLLHAATDRIENVPQSKIDQADEWLEKGASRISV
                     VSADPDAFAGVDSHRVASYQSANGKALMNLRKATQANKVSWTVVAAAGKQWAAKVFPE
                     LPEEKQVEALWDQIFKTTRVYEEDPVLAWKKHDEKLAKKAEELNREQFSALHYTAPGT
                     DIIIGLPKNHLWEGAGSYNARGEKFMANMPTEEVFTAPDSHRVDGYISSTKPLSYAGT
                     IISGMKFTFKDGKVVDFSAEQGEDVLAKLLDTDEGARRLGEVALVPDPSPISQSGIIF
                     FNTLFDENASNHLALGSAYAFSVKGGTEMSDEELAEAGLNRSQTHVDFMVGSDKMDID
                     GIREDGSTVPIFRNGDWA"
     gene            1591..2160
                     /locus_tag="ACG842_001315"
     CDS             1591..2160
                     /locus_tag="ACG842_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFQ0247868.1"
                     /translation="MARKKTITREQILAAAYEVAATDGFTRFTARNIANKMNCSTQPI
                     YLEFKNMDDLRQALFEQIYDHLQYEVFTVEHTGNTIIDLAFNYIHFARREKKLYRSLY
                     LEEYGGGKEMQNFSYTYFVNTVKRDPAYVNLSEEQIESLYNGTWIIATGIAALMSSSI
                     IHPTDEQIEKMIQDSIDAILKFKEPIEID"
     gene            complement(2338..2778)
                     /locus_tag="ACG842_001316"
     CDS             complement(2338..2778)
                     /locus_tag="ACG842_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HFQ0247869.1"
                     /translation="MALAKIVYASMTGNTEEIADIVAEAFENLDIEVEINECTQVDAD
                     EFEEADICVVATYTYDDGDLPDEIVDFYEDLQELDLLGKIYGVCGSGDTFYDEFCKSV
                     DDFDAAFAKTGASKGAENVKVDLNAEEEDIENLEAFAKELVAKI"
     gene            2936..3700
                     /gene="map"
                     /locus_tag="ACG842_001317"
     CDS             2936..3700
                     /gene="map"
                     /locus_tag="ACG842_001317"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008377375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HFQ0247870.1"
                     /translation="MITLKSAREIEMMAESGALLADVHKNLRDFIKPGITSWDIEVFV
                     RNYIESHGGIAAQIGFEGYEYATCISINDEICHGFPRKKPLKNGDLVKVDMCIDLKGG
                     ISDSCWAYVVGESTPEIDRLMEVTKKALYIGIEQAQVGNRIGDIGHAIQTYVEGENLA
                     IVRDFIGHGVGPTIHEEPAVPHYGEAGKGLRLKEGMVITIEPMVNTGTWKMKMDPNGW
                     TAYTRDGGLSCQYEHTLAITKDGPRILTSQGEEGTY"
     gene            3732..4655
                     /locus_tag="ACG842_001318"
     CDS             3732..4655
                     /locus_tag="ACG842_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HFQ0247871.1"
                     /translation="MKVWNKIKQNENLMRFIETTQKRITDSEMSTTSVVVAYYLLLSL
                     FPLIIAFGNILPFLHIDQETVLTYLREVIPETVYQFIGPAIKDLLTQSSGGLLSVSAL
                     AALWSASQSINALQIAMNKAYGVENRKNFLIVRFFSLIVIVLFMIAISGVTIVLGLGQ
                     LILDAIQPIVQIPVNFIDQFQTLKWPITLVALFVIMFLIYLIVPNAQLKLKTIIPGTI
                     FATVGWMLLSQVFSIYARYFATRISGYQIIGSFIVLMLWLNFAATIIILGGIINAVVQ
                     EYVTGHQIKKRRDPSRKWFNKLKTKFSHKKK"
     gene            4731..5870
                     /locus_tag="ACG842_001319"
     CDS             4731..5870
                     /locus_tag="ACG842_001319"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HFQ0247872.1"
                     /translation="MLVSFSMVIRFFLTILLSLILTPVFKIISVQTGMVDKPNERRIN
                     KVPMPSAGGLPIFISFVISTLFLFRKIIPQSYIVPILIASLVIIVTGVLDDKYELTPK
                     QKSIGILIASLIIYFVADIRIDSFTLPFIGYIQLGWLSFPVTIFWIFGITNAVNLIDG
                     LDGLAAGISLIGLITIGIIGYFFLHASTVYIPVVIFCLVASIIGFFPYNFYPAKIYLG
                     DTGALFLGFMMAVLSLQGLKNVTFVSSISLLIVMGVPVTDTFFAIIRRKANRVSFSTA
                     DKKHLHHRLISLGFTHKGAVLTIYAMALMFSFTAMVMNYTGRIGTIILIIAMLFAAIL
                     LFELIGLINEKHQFILKTLRFLGNKEYRTQVKQKYLKKWNRHIDE"
     gene            5863..6663
                     /locus_tag="ACG842_001320"
     CDS             5863..6663
                     /locus_tag="ACG842_001320"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFQ0247873.1"
                     /translation="MNNKLNQPILISIVTANSDKIFRTLDNFISTVKDETAVKIRIFD
                     NNSLPEYKKRLTEYRKYPYIDIYFHDENSGFGYGHNHNLIASDSRYGIICNPDILVDE
                     ETVTALVLLLKEHPECAMLAPKILNEDGTTQHLIREQLAVFDYVLRFVPFKWVKKKFD
                     KRLAVFECRGLPTDANSYVRMISGSFMVADIDKFKEIKGFDERFFMYFEDNDLCLSFE
                     KAGYKLLYTPFYAVIHFYGKGAHRNAKLFRVFLTSMMKFFNKWGWRFF"
     gene            6663..7490
                     /locus_tag="ACG842_001321"
     CDS             6663..7490
                     /locus_tag="ACG842_001321"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFQ0247874.1"
                     /translation="MKPEILVVVVLYQQLFSQSPAYGPLSKALSEKQIQLIIYDNSPQ
                     RQQNELFEKENIVYHHDPQNPGLAVAYNFALSQADEGTRCLVTLDQDTRLVDDYFELL
                     RKVTFTDECVAAVPMIFSGSRQVSPLYSDRYINRTAQAVEVEGPTAERIMAINSGVAW
                     SVKFLKKIGGFNPAFSLDFLDHWLFWKVDQLEKTVEILPVRMEHDLSVLDYEKVSSNR
                     YRSILQAENRFYHEYDQRHLSSHRRQLLLRTAKQFLTVKNRHIWRMTLRSYVKNWKV"
     gene            7495..8202
                     /locus_tag="ACG842_001322"
     CDS             7495..8202
                     /locus_tag="ACG842_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFQ0247875.1"
                     /translation="MRSVCIATFNGGRYLREQLDSILSQLSAEDEVVISDDGSVDDTW
                     DILTEYAEKDKRIQLFSGPRQGLIANFGYAIQQTKGDLIFLADQDDVWLETKVKKITD
                     YFEQNPDQLVVVSDLIIVDSELQEIHDSYFSFRNSKEGWLNNVIRSHYIGAGMAFRSS
                     LKPDILPIPSEVPMHDMWIGLLGGKKTGFLREPLTLYRRHDLNASEIETHSKLRQKIS
                     WRVHLITALIKRKWFKR"
     gene            8302..9168
                     /gene="rfbA"
                     /locus_tag="ACG842_001323"
     CDS             8302..9168
                     /gene="rfbA"
                     /locus_tag="ACG842_001323"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HFQ0247876.1"
                     /translation="MKGIILAGGSGTRLYPLTKATSKQLMPIYDKPMIYYPMSTLMLA
                     GIKEILIISTPQDTPRFKELFGNGNDLGLQIEYAVQESPDGLAQAFIIGEEFIGDDSV
                     CLVLGDNIYYGGGLSKMLQRAASKDTGATVFGYHVNDPERFGVVEFDENMQALSIEEK
                     PAQPKSNYAVTGLYFYDNEVISIAKGIKPSERGELEITDVNKAYLDKGKLSVELMGRG
                     FAWLDTGTHESLLEASTFIETIEKRQNLKVACLEEIAYRMGYIDKEQLLALAQPLKKN
                     QYGQYLLNLAAE"
     gene            9182..9754
                     /gene="rfbC"
                     /locus_tag="ACG842_001324"
     CDS             9182..9754
                     /gene="rfbC"
                     /locus_tag="ACG842_001324"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010771301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HFQ0247877.1"
                     /translation="MKVTDTKLTDVKIVEMDVFGDHRGFFTESYSKAKFAEHGLKYDF
                     VQDNHSLSAEAGVLRGLHFQKGEAAQTKLIRVVTGAVLDVVVDIRKGSPTYGQWDGFI
                     LSEHNHRQLLVPKGYAHGFVTLTPNVNFLYKCDNYYNAEADGGIAFDDPDLAIDWPID
                     PSRAIMSEKDHRHPTLKEFEAENPFVYGEI"
     gene            9776..10804
                     /gene="rfbB"
                     /locus_tag="ACG842_001325"
     CDS             9776..10804
                     /gene="rfbB"
                     /locus_tag="ACG842_001325"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HFQ0247878.1"
                     /translation="MKNIIVTGGAGFIGSNFVHYVVNNHPEVHVTVLDKLTYAGNKEN
                     LAGLPSDRVELVVGDVADAELVDRLVKEADAVVHYAAESHNDNSLKDPFPFVQTNIIG
                     TYTLLEACRKYDVRYHHVSTDEVYGDLPLREDLPGHGEGEGEKFTAETPYNPSSPYSS
                     TKAGSDLLVKAWVRSFGLRATISNCSNNYGPYQHIEKFIPRQVTNILSGIRPKLYGEG
                     KNVRDWIHTNDHSSAVWLILTKGRIGETYLIGADGEEDNKTVMELILEMMGQPKDAYD
                     HVNDRAGHDLRYAIDSTKLREELGWKPEFTNFRDGLADTIKWYEEHEDWWKKEKEAVE
                     AAYAKNGQ"
     gene            10902..11753
                     /gene="rfbD"
                     /locus_tag="ACG842_001326"
     CDS             10902..11753
                     /gene="rfbD"
                     /locus_tag="ACG842_001326"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HFQ0247879.1"
                     /translation="MFLLTGGNGQLGTELRHLLDEKNLQYVSTDAKDLDITDAEKTMA
                     YITELKPDVIFHCAAYTAVDKAEEEAKELDEKINVDGTRNVALAAKAVNAQFVYISTD
                     YVFDGKNKNVEYEVDDQTNPLNEYGRTKLLGEKAVEEILEDYYIIRTSWVFGIYGHNF
                     VFTMQRLAETRDKLTVVNDQFGRPTWTRTLAEFMVYVIEEKAPFGIYHLSNENSCSWY
                     EFAKEILKDTDVEVAPVTSEEFPQKATRPQYSVMSLKKTEELGFVIPTWQEALAQMLE
                     AAKKMNK"
     gene            11787..13820
                     /locus_tag="ACG842_001327"
     CDS             11787..13820
                     /locus_tag="ACG842_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0247880.1"
                     /translation="MEKQTRSARRKEKPKANQADSNLVLDLFLKLRYLIGVLVIVVIV
                     TFNLNGSSLGVWDKYVSQRDDGKKTDVIFGQNREVRSDEWLVQTPFYLSQAEEDFPLV
                     NEDYSLNGQNMIIAYNSPVKDITVIGKPFNWGFFFLGRDRGLSFYWAMKLVVMVLLGF
                     EVLMILTKRKKALSLIGAFALTFSPAVQWWFMQHVGDLIFFTLGLMTAFYHYFYNHDK
                     KWLRTLMMLLVVIFGIGFILVIYPAHQVMLAYLLIFYFVGLLIYYSKKVSWDLLDVVL
                     IGGAVLFIGGVMVHFWLTSKDALLASLNTLYPGKRVSTGGKWTIGEFFSFLTNWKIPF
                     EDINYSNNSEVALFYHFFPTVFLASPFVLFGKKDSEQKLLGRILMIFCLFSIFWITVG
                     LPKWLAEITLLSYVPPLRAILTFSFAACLLSIWFIAYIWREDVIPIWYKFILLAVNAW
                     SYFYAITHSKMDNYFSDFETIAVAVLGTVILAFALFGWRRLFYLAFAALVIVSGFFVN
                     PVVEGTGAIFEKTLAKEIQKIDQQDPGKVWLSEDELYNFTPTLGVKSFNTVRFYPDIA
                     AWKKIDPKEEYEKYYNRYAHTRAFIAKEDTKFILDRPDMFSVTLNFEDAEKLGIDYVI
                     SKRPLTDYNQLYEAQFTQLYGPDKDGYMIFKVEYPDYPDVPQTQTQENQLVVQ"
     gene            13864..15144
                     /locus_tag="ACG842_001328"
     CDS             13864..15144
                     /locus_tag="ACG842_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpaQ family protein"
                     /protein_id="HFQ0247881.1"
                     /translation="MEIIQAIVQKFVNKMSVGFLLLLLTLVFFVWTAGLGFPATLFIY
                     NNSSLLIAFSVAILLILNVYKAKGVDFLYLGVALALFIFFSYFRELRNESLFGDLLFP
                     LIILFCLVIKWIEWTKWDRGIYLIVFSVFLGITLYRVFTEIQVPEGQSIWAPGNSLSD
                     IWINTNIIGSSLMTLALLISGFASSFERWYVRIIGAVAVVAAFLGIWVCQSRGALVAL
                     IVFAILDLLPKSLMRVIRAPFIAYTVTILLALPISYLAAVSEKVNLFTGREDIWHKFY
                     QTLGEKSEQILLGMKTFIFQRGNQFLGNHNSYNSILNIYGLIGFGIAALLLILFIGRL
                     TLKADLSNGQMTFIWAFFAVMMQSFMEDTLTSYPWVPIIYILLAMAVHRYDEPEVAEE
                     FYVMEEAYDDMTYDDTSYEEQPDSAHSSRIQRYH"
     gene            15354..16160
                     /locus_tag="ACG842_001329"
     CDS             15354..16160
                     /locus_tag="ACG842_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0247882.1"
                     /translation="MFKDLYLFMKDIYQNRKLLLQFSINDFKSRYAGSFLGILWAFIN
                     PLFTVLIYWLVFGVGLKAQMIDAKYPFIVYLVTGMIPWFFFSDAFASTTVVFREYTYL
                     VKKVVFNIRILPTTKILSNLYTHLFFILIGIAVALINGIYPSAMSLQLIYYLFCMCAF
                     LTGLTWLTASIQPFLPDIMQLITIVLQLIMWTLPILWSPNQFDSGLVSILKLNPLYYV
                     VQGYRESFLSEAWFWEHGTYTLYFWVFTLVMFVIGSAVFRRLKPHFSDVL"
     gene            16172..17392
                     /locus_tag="ACG842_001330"
     CDS             16172..17392
                     /locus_tag="ACG842_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0247883.1"
                     /translation="MTEYAIRLKDITKSYNMYAKPSDRFREALNPFKKSYHDVFYALK
                     NVNVEIKKGEMIGFVGENGSGKSTMLKIITGVLTPTSGTMEIEGKISALLELGSGFNP
                     EYSGYENIYLNGMVLGFSREEVDEMVDDIIEFADIGDHIYQPVKTYSSGMFVRLAFAV
                     AINVNPDILIVDEALAVGDLEFQLKCMEKFTEIKNSGKTILFVSHDISSIRRFCDRSY
                     WLKNGEVVEYGDTMEVTTNYENFLKKKSVKTVDRNKNQVERFAAPDIVDVTKAELLDK
                     DKNPVDMLEQGEKLYVKVTYEVKDDTIKNPVLGIALRTVDNFYVCGLNTLLDGIKIPW
                     KKGENIFYLEYEKLGLLAGEYYFDVAVFEENATVPLVYKTKYMNLFVSGSYIGEGIVV
                     LDHKWEEGTHSNEI"
     gene            17382..18968
                     /locus_tag="ACG842_001331"
     CDS             17382..18968
                     /locus_tag="ACG842_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFQ0247884.1"
                     /translation="MKYDFEMDLDEQSSVGKIAAQIKPGSKVLEFGPGNGRLTKHLIG
                     AKQCEVSIVELDKELFDFVSEFAQDGFYGDIESFEWANYYAGQTFDYVLFADVLEHLV
                     DPGKTLKKVREFLNEEGEILITFPNLAHNSVMIDLFNNQLPWASYGLLDETHNSFYTH
                     DGFQKVFEKAGLFINIEDYLYLAVGDTELKSTYEELPEAVRYDFKMRPFGEVYQYFFS
                     LMKHPVAQSSIAEPQNSNYVKVLEVTQQTKQDETIQQIPFNNFTGENETLSFPVSETT
                     ERMVFRFAQQPSFIEFSAEAAGEKLTFIDSNAVVKTVNDCYLFDGKELPEFVLTDISG
                     KEVTIHCHYRFIGELTPTMKELLETIRPMAEVTKQLSEKNDELERENHHLLEENTRLD
                     HTLKTTTNRYCTLLESDEWTIKHRLGRRKKETSKKIQEKELSICIDEKIWDAETKILK
                     IIGWGIANSDRQPLSYKLSADQSPFFEAIPVSREEVNQAEQLAKGTEAGFELRILCEQ
                     ERSFLVEAVAQNGQSWIIEM"
     gene            19007..21145
                     /locus_tag="ACG842_001332"
     CDS             19007..21145
                     /locus_tag="ACG842_001332"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFQ0247885.1"
                     /translation="MQKKEIAVFIDQITRERATGDLLIVGWAIDEVTKEIPTIKVEKE
                     NVIAEATHVVRLDINHLYNLDVKTQSGFKVRLSGKMRGKAILDFQTARHQNGIAVKLN
                     GKYPYDDGIESSWERKKRLLKKGINYARTHGVKKAIRRVKLELKPGSIDYAEWISRHE
                     KPDLKEQRKEAQGFAYRPLISILMPVYNVEIKWLEKCIDSVLDQTYDHWELCISDDAS
                     TDPAIRKCLESYQAKDDRIKVVFRKENGHISLATNSALEIAEGEFIALLDNDDELPPF
                     ALYEVAKVLNVHPELDVIYSDEDKIDADGNRFDPHFKADWSPDTLMGNNYISHLGVYR
                     TSIVKELGGFRKGYEGSQDYDLVLRVTEQIPADHIYHIDRVLYHWRTIPGSTASNGEA
                     KSYIYDSGVKALTDALSRRNIKGSVRPGRISGFYEIAYDVLQEDLVSVIIPTKNGYED
                     LKTCVDSIIEKTSYSNYEIIIADNGSTDPKMQELFAEYKHQLKDRFIVELIDIPFNYS
                     RINNLAAEKANGKYFLFLNNDTEVIEPDWMTVMVSYAQFDRIGCVGAKLFYPDDTTQH
                     AGVLLGIGGVAGHALNNYDRTHCGYFGRLVIDVNYLAVTAACMMVKAADFNAVNGFDE
                     TLEVAFNDVDLCLKVYELGRYNVYAHQAELYHFESKSRGYEDTPEKQRRFAGEIKKMQ
                     DKWPAYIAHDPFYNDNLTKEGIGDFSLRPD"
     gene            complement(21513..22496)
                     /locus_tag="ACG842_001333"
     CDS             complement(21513..22496)
                     /locus_tag="ACG842_001333"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HFQ0247886.1"
                     /translation="MNKQELHDFIGFRQPNICQIVAYKDGVEVYTDEWNGYKKDDTCH
                     IMSATKSIVSLLIGIALEQGLIKSIDDKVLDYFPEYKVKRGEKTIYAITIKHLLTMKA
                     PYKCKGDPWTKVCSSEDWTKTSLDLLGGRKGITGEFNYQTVCLHILTGLLYQTSKLTT
                     VDFANKYLFDPIGIPNHVNYYAETAEEHKQFTMDKSPKTNVWFCDPLNIGTAGYGLCL
                     SASDLAKIGVLCLNKGMYKGKQIVPPMWINEMFKPRTSALEDLQNMRYGYLWWVLDSE
                     NEIYSAIGNSGNVLYIDAKNNLVISITSYFKPMIIDRVAFVEEYLKPFILK"
     gene            22874..24265
                     /locus_tag="ACG842_001334"
     CDS             22874..24265
                     /locus_tag="ACG842_001334"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HFQ0247887.1"
                     /translation="MNKNGEWSAARRIFIILMDVIVYNAAIYSSFLLKFRGEIPLRNL
                     ETFQHSAIIISVIFIALNILLGAYVFYNRMISDIVFVTVIGQVLMTLGIMVVTFAGRW
                     FAFPRLVILLSFVLGTLFLSIYRILVYKLYLRVSRDKKVVIIGLEKDVAPAIQNFGSK
                     KNNKHKVEAVILDNYYANIKNMIKDIDIVYLASHIEETEKIKIYQLVTKNEKKLFLNT
                     TFENLTLINPNIMNFEDESIIEASGFRIQPEYELFKRLFDIIVSLVLLVIASPFMLFT
                     AILVKVTSPGPIIYKQVRITKNQKEFSIYKFRSMTATAEAKSGPVLAKSNDARVTPVG
                     KFIRAVRFDELPQIFNVLKGDMSIVGPRPERPFFVDQFNAENPYYYLRHNVRAGITGY
                     AQVYGKYVSDYNSKLRFDLLYIKKYSLMLDLKILLQTIKILFDKMSSQGLEEDENVIS
                     LAEIAFPNEKIFR"
     gene            24281..>24862
                     /locus_tag="ACG842_001335"
     CDS             24281..>24862
                     /locus_tag="ACG842_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFQ0247888.1"
                     /translation="MNNPKFSIIVPVYNAEATIGRTIDILKELDYEEYEVVLVNDGST
                     DQTQEIIQELIENDERFELITTTNQGPGLARNEGIRQSKGEYLLFFDADDTPVKTILS
                     DYDHLLEKSPKADLVVSSFVFRTMDEGRIVSEKENLVTECHYLTHEEFIKDMYQLMNE
                     QLMYVVWNKCYRSDILKNNQILFKTYNSCEDRIF"
BASE COUNT         7860 a         4223 c         5080 g         7699 t
ORIGIN      
        1 aaaaaacggt ggggttgttt agcgaaataa aggatctaag ccaatagctt agatccttta
       61 ttctatgctt tatattattt tttgcatgtg tgcgaaagta tactcaagcc caatcgccat
      121 ttcggaaaat cggtactgta gagccatctt cacggattcc gtcgatatcc atcttgtcag
      181 atcctaccat gaaatctacg tgggtttgac ttctattcaa tccagcttct gctaattctt
      241 catcggacat ttctgtaccg cctttcacac tgaaggcata agcagaacct agtgctaagt
      301 gattcgatgc gttttcatca aataatgtat tgaaaaagat gatgccagat tgagaaatag
      361 gtgaagggtc aggtacaagt gcgacttctc ctaaacgacg cgcaccttca tctgtatcaa
      421 gcagttttgc taagacatct tctccttgtt cggcagaaaa atcaacaact tttccatctt
      481 taaaagtaaa tttcataccc gagatgatgg tgccagcata actcagtggc tttgtacttg
      541 aaatgtaacc atctactcga tgactgtcag gtgctgtgaa cacttcttcc gtcggcatat
      601 ttgccatgaa tttttctcca cgagcattat agcttccagc gccttcccaa agatggtttt
      661 taggcaatcc gataatgata tctgtaccgg gagcagtata gtgaagggca gaaaattgtt
      721 ctcgattcaa ttcttctgct ttttttgcta atttttcatc atgcttcttc catgctaaga
      781 cagggtcttc ttcatagaca cgagtcgttt tgaaaatctg atcccataat gcctcaactt
      841 gtttttcttc tggcagttct gggaagactt ttgctgccca ttgtttacca gccgcagcca
      901 cgacagtcca actgacttta tttgcttgag ttgccttgcg taaattcatt agtgctttgc
      961 cattcgctga ttgataagac gctactcgat ggctatctac accagcaaat gcatcgggat
     1021 cggcagaaac aacgctgatc ctgcttgctc ctttttcaag ccattcatcc gcttgatcga
     1081 ttttagattg tggtacattc tcaatacgat ccgttgcagc atgtaaaaga aattctcttt
     1141 ggatctggtc gtcagtccat tgaacgataa cctcagcggc tcctagttta taagcttctt
     1201 gtgtgatcaa tcgagcaagg ggtgcctgtt ccacgctgat ctgtaaaaca actgtgtgtc
     1261 ctttttctgt ggctacgcca gtttctgcaa ttaagcgtgc atattttttt agaagttcat
     1321 taaaatcaga taacataata aaaattcctc ctcaccattt tttctggtaa ctattagcat
     1381 gataccattt aattatgatt caaacaatga ggctttaaat aaaaaaagga ggttattttt
     1441 ttaaggttaa gtttcatgat atatgttact acttgctatt tcagcttgtg aagaaaatca
     1501 aaaactaatt ttgcatatca aaaaaaacat gttacaatga aattaatcaa tggtggaagc
     1561 gttataaaaa aattaaggag attaaaaaat atggcaagaa aaaagacgat tactagagaa
     1621 cagattttgg cagctgctta tgaagtagct gccaccgatg gatttacacg atttactgcg
     1681 agaaacatcg cgaacaaaat gaattgttcc acgcaaccga tttatcttga gtttaaaaat
     1741 atggatgact tgagacaagc tctttttgag cagatctatg atcatttaca atatgaagtg
     1801 ttcactgttg agcatacagg gaatacgatt atcgatcttg cttttaatta tatccatttt
     1861 gcaagaagag agaagaagtt gtatcgttca ctgtacttag aagaatatgg tggaggaaaa
     1921 gagatgcaga atttctctta tacttacttt gtgaatacag tgaaacgcga tccagcttat
     1981 gtgaatcttt cagaagagca gatcgaatct ttgtataatg ggacatggat catagctact
     2041 gggatcgcag ctttgatgtc ttcaagcatc atccatccaa ctgatgaaca gattgaaaag
     2101 atgatccaag acagtataga tgctatcttg aaattcaaag aaccaatcga aatcgattga
     2161 atatactcaa aagttccaca aatcagtagg taacaagaga tgtgtcaaaa taaggaaaag
     2221 ttcgtttttc ttacagcgta tgcattttta actcccgaag aactcttcca tgaatgcaat
     2281 gaataaaaga gccatacaga ttgaataaat ctggatggct cttttattgc aagtatatta
     2341 aatttttgcg actagttctt tagcgaaagc ttctaaattt tcgatatctt cttcttcagc
     2401 attcagatcc actttaacat tttcggctcc ttttgatgcg cctgtttttg caaaagcagc
     2461 atcgaaatca tccaccgatt tgcagaattc atcgtaaaaa gtatcaccag atccgcagac
     2521 accgtaaatt tttcccaata gatccaattc ttgcaagtct tcataaaagt ctacgatctc
     2581 atcaggcaga tcgccatcat cgtatgtata agtagctact acgcaaatat ctgcctcctc
     2641 gaattcatct gcatctactt gggtacattc gttgatttcg acttcgatat ccagattttc
     2701 aaatgcttct gcaacaatat cggcgatttc ttcagtgttt cctgtcatgc tagcatatac
     2761 gatttttgct aaagccatga tgtgcctcct cgtttataat tatttaacaa ttatgaatta
     2821 taccaaaaca tcattgaaaa gaaaataaaa cctagagaca cctggggaat gtcatttggc
     2881 taaatgtatg ccgctatgtt aaaatgagag ggatttgtaa aaaggagacg aaaatatgat
     2941 tactttaaaa tcagcacgtg aaattgaaat gatggcagaa tctggagctt tattagcaga
     3001 tgtacataaa aatctgcgtg attttataaa accaggaatc acaagttggg atattgaggt
     3061 atttgtcaga aactatatcg agagtcatgg cggaattgct gcgcaaattg gctttgaagg
     3121 gtatgaatat gcgacttgta tcagtataaa tgatgaaatt tgtcatggat tccctcgtaa
     3181 aaagcctttg aaaaacggtg atttggtaaa agtcgatatg tgtatcgatt tgaaaggcgg
     3241 catttctgat tcttgttggg cctatgtagt tggagaatct acacctgaga tcgaccgatt
     3301 gatggaagta acgaagaaag ctttgtacat cggtattgag caagcacaag tagggaatcg
     3361 gattggtgac atcggtcatg ctatccaaac ttatgtagaa ggcgaaaatc tagcgatcgt
     3421 tcgtgatttt attggtcatg gcgttggtcc tacgattcat gaagaaccag cagttcctca
     3481 ttacggagaa gctggaaaag ggttgcggtt gaaagaaggc atggttatca cgatcgaacc
     3541 tatggttaat accggaactt ggaaaatgaa aatggatcct aacggctgga ctgcgtatac
     3601 aagagacggc ggactttctt gccaatatga acatacattg gctattacta aagatggtcc
     3661 aagaattttg acatcacaag gagaagaagg cacttattga tctatccagt caataggtac
     3721 gggagaggta catgaaagtt tggaacaaaa taaaacaaaa tgaaaatctg atgcgcttta
     3781 ttgagacgac tcaaaagcgc atcactgatt cagaaatgag tacgacctct gtcgtcgttg
     3841 cttattattt actcttgtct ttatttccat tgattattgc ttttggtaat attttaccgt
     3901 ttctccacat cgatcaagag actgtactga cctatttacg agaagtgatt cctgagaccg
     3961 tttaccagtt tatcggtcct gcaatcaaag accttttgac acaaagttca ggaggcttgt
     4021 tatccgtttc tgcattagcg gctttatggt ctgcaagtca aagtatcaat gctttgcaaa
     4081 ttgcgatgaa taaagcgtat ggcgtagaaa atagaaaaaa ttttttgatc gtccgtttct
     4141 tctcactaat tgtcattgtc ttgttcatga ttgctattag tggggtaact atcgttcttg
     4201 ggttgggaca gctgattttg gatgctattc agccaatcgt acaaataccc gtgaacttta
     4261 tcgatcagtt ccaaactttg aagtggccaa tcacattggt agctttattt gtcatcatgt
     4321 tcttgattta tttaattgtc cctaatgctc agttgaagtt gaagacgatc attccgggca
     4381 cgatcttcgc tactgtcggc tggatgctgt tatcacaagt gtttagtatc tatgctaggt
     4441 attttgcaac gaggatcagc ggttatcaga tcataggaag ttttattgta ctcatgcttt
     4501 ggcttaattt tgctgctacg atcatcattt tgggcggaat catcaatgca gttgttcaag
     4561 aatacgtgac gggtcatcaa atcaaaaaac gtcgagatcc aagccgaaaa tggttcaata
     4621 aattgaaaac taaattttct cataaaaaaa agtagaaaca agagttctta ttgtaaattt
     4681 atatcagtaa tttataataa ttatgactct taattaaagt aggtgcccat atgcttgttt
     4741 cttttagtat ggttataaga ttttttctga caattttatt atcactgata ttaacacctg
     4801 tattcaaaat catttcagta caaacaggta tggtcgacaa accaaatgaa agaagaataa
     4861 ataaagttcc aatgccttca gcgggcggac tgcctatctt tatttcattt gttatatcga
     4921 ctttattctt attccgtaaa attatccctc aatcgtatat tgtaccgatc ttgattgctt
     4981 ctttagtaat catagtcaca ggagtgctcg atgataaata tgagctgaca cctaaacaaa
     5041 aaagtatagg gatcttgatt gcttctctta ttatttattt tgtagcagat atacgtattg
     5101 attccttcac gcttcctttt attggatata tccaactagg ctggcttagt tttcccgtaa
     5161 cgattttttg gatcttcggt atcacgaatg cggtcaatct gattgacggt ttggatgggc
     5221 tggcggcagg gatttcattg atcggtttaa ttacgatagg aatcattgga tattttttcc
     5281 tccatgcttc tacagtgtat attccagttg ttattttctg tcttgtagca agtattatcg
     5341 gtttttttcc ttacaatttt tatccggcaa agatttattt aggcgataca ggagctctat
     5401 tcctagggtt tatgatggcc gtcctttcac tacaagggct gaagaacgtg acttttgttt
     5461 catcgatttc tttgctgatc gtcatgggag tgccggtaac agacacgttt tttgctatca
     5521 ttaggagaaa agcaaaccgt gtttcttttt ctacagcaga taaaaaacat cttcatcacc
     5581 gactaatcag tttaggcttt actcataaag gtgctgtcct gacgatttat gcgatggctt
     5641 tgatgttttc tttcacagct atggtcatga attatactgg aagaatcgga acgatcatat
     5701 tgattattgc catgttgttt gctgcaattt tattatttga attgattggt ctgatcaatg
     5761 agaaacatca gtttatttta aaaacattgc gatttttagg gaataaagag taccgtacgc
     5821 aagtaaaaca gaaatatttg aagaaatgga accgtcatat agatgaataa caaattaaac
     5881 cagccgatcc ttattagtat tgtgacggca aacagtgata aaatttttcg gacactagat
     5941 aattttattt caactgtaaa agatgaaaca gcagtaaaaa tccgaatttt tgacaacaat
     6001 tcacttcccg agtacaaaaa aagactaaca gagtatagga aatatcccta catcgatatc
     6061 tattttcatg atgaaaattc cggatttggt tatggacata atcacaatct gatagctagc
     6121 gattctcgct atggcattat ttgtaaccca gatattttag tggatgaaga gacagttaca
     6181 gcattggtct tgttattgaa agaacatccg gaatgtgcga tgttagcacc taagattctt
     6241 aatgaagatg gaacaactca gcacctcatc cgtgagcagc tggctgtttt tgattacgtt
     6301 ttgcgctttg tgcctttcaa atgggtcaaa aaaaagtttg acaaacgact ggctgtgttt
     6361 gaatgccgag gattgcctac tgatgctaat agttatgtac ggatgatctc aggtagtttt
     6421 atggtagctg atattgataa atttaaagag ataaaggggt ttgatgagcg tttcttcatg
     6481 tactttgaag acaacgactt gtgtttgagt tttgaaaaag caggttacaa gttattgtac
     6541 acaccctttt atgcagttat ccatttttac gggaagggtg cgcatcgaaa tgcaaaactg
     6601 tttcgcgtgt ttttgacttc tatgatgaaa tttttcaata agtgggggtg gcgttttttc
     6661 tgatgaaacc agaaatatta gtagtcgttg tgctttatca gcaactattc agtcaatctc
     6721 cagcttatgg accattaagc aaagcactta gcgaaaagca aattcagctt attatttacg
     6781 ataatagtcc tcaaaggcag caaaatgagc tgtttgaaaa agaaaatatc gtttatcatc
     6841 atgatccgca aaatcctggt ttagcggtgg cttataattt tgcgttgtct caagcagatg
     6901 aaggaacacg ttgtctcgtc actctggatc aggatacgag gctagtcgac gattattttg
     6961 aactattgcg taaagtaacc tttacagatg aatgtgttgc tgcagttccg atgatttttt
     7021 ctgggagccg acaagtttct cccctatatt cagatcgtta tatcaatcgt acagctcaag
     7081 cagtagaagt tgaaggccca acagctgaac gaatcatggc aatcaattca ggtgttgcct
     7141 ggtctgtcaa atttctaaaa aagattggcg ggtttaatcc tgctttttct ttagattttt
     7201 tggatcattg gctgttttgg aaagtggatc agttggaaaa aactgtggag atccttcctg
     7261 tacgaatgga acatgatctt tcggttttag actatgaaaa agtttcaagt aatcgatacc
     7321 gttctatttt acaagcagaa aatcgatttt atcatgaata tgatcaaagg catttatcca
     7381 gtcaccgacg acaattattg ttaagaacag ctaaacagtt cttgaccgtc aaaaaccggc
     7441 atatctggcg gatgactttg cgctcatatg ttaagaattg gaaggtgtga attcgtgcgt
     7501 tcggtttgta tagctacctt taatggaggc agatatttaa gagagcagtt agatagtatt
     7561 ttatctcagc tttcagcaga agatgaagta gtcatttctg atgatgggtc agtagatgat
     7621 acatgggata tattaactga atacgcagaa aaagataagc gaatccagct tttttcgggg
     7681 cctcgtcaag gattgatcgc taattttggt tatgcgatcc agcagacgaa aggagatcta
     7741 atctttttgg cagaccaaga tgatgtttgg ttggagacaa aagtgaaaaa gatcactgac
     7801 tattttgagc agaatccgga tcagctagta gtagtttctg atttaatcat tgtggacagc
     7861 gagctgcaag agatccatga ttcgtatttt tcgtttcgaa attccaaaga aggatggctt
     7921 aataatgtga taagaagtca ctatattggt gctggtatgg ctttccggtc ttctttaaaa
     7981 cccgatattt tgccgatccc gtcagaagtg ccgatgcatg atatgtggat tggtttgctg
     8041 ggaggaaaga agactgggtt cctaagagaa ccgttaactc tgtaccgaag acatgattta
     8101 aacgcgagtg agattgaaac acattctaaa ttacggcaaa aaatttcttg gcgtgttcat
     8161 ctgattacgg cgttgatcaa acgaaaatgg tttaaacgtt gataaaagat gtttaaatcc
     8221 ttaatatttc catgacaaat ataacaattg atgagataat aacaaagtga aatttgaaat
     8281 caaaaaggag agcagatatc catgaaggga atcattcttg ctgggggcag cggaacacgt
     8341 ctgtaccctt taacaaaggc aacatcgaaa caattgatgc cgatttatga caaaccaatg
     8401 atttattatc caatgtctac tctgatgttg gcgggaatca aagaaattct gattatttca
     8461 acgccacaag atacaccacg tttcaaggaa ttatttggaa acggaaatga tttaggactg
     8521 cagatcgaat atgcagtaca agaaagtcca gatggattgg cacaagcgtt tatcattgga
     8581 gaagaattta ttggagatga tagtgtctgc ttggtattag gggacaatat ttactatggt
     8641 ggcggtctgt ctaaaatgtt gcaacgagca gcttcaaaag atacaggcgc gacagtattt
     8701 ggctatcatg tcaatgatcc agaacgcttt ggtgtagtag aatttgatga gaatatgcaa
     8761 gctttgtcga ttgaagaaaa gcctgctcag ccaaaatcaa attacgctgt aactggcctt
     8821 tatttttatg acaatgaggt aatttctatc gctaaaggta tcaaaccttc agaacgtgga
     8881 gaactagaga ttacggatgt caataaagca tatttggata aaggaaaact atccgtagaa
     8941 cttatgggac gcggatttgc ttggcttgat acaggaacac atgaatcatt gcttgaagca
     9001 tctaccttta tcgaaacaat tgaaaaacgt cagaacttaa aagtggcttg tttggaagaa
     9061 attgcttacc gtatgggcta catcgataag gaacaattgc tggcccttgc acagccattg
     9121 aaaaaaaatc aatacggtca atatttattg aatttggccg ctgaataagg agtccttaca
     9181 gatgaaagta acagatacaa aattaacaga tgtcaaaatc gtagaaatgg acgtctttgg
     9241 cgatcatcgc ggtttcttca cagaaagtta ttcaaaagca aaatttgcag aacatggatt
     9301 gaaatatgac tttgttcaag acaaccattc actatcagct gaagctggtg tccttcgggg
     9361 gcttcatttc caaaaaggag aagctgcaca gacaaaattg attcgggtag tcactggagc
     9421 agttttagat gtagtagtgg atatccgcaa aggtagcccg acttatggtc aatgggacgg
     9481 atttatctta tctgaacata atcatcgaca gttgcttgta cctaaaggat atgcccatgg
     9541 attcgttact ttgacaccaa atgtgaactt cctttataag tgtgataatt actataatgc
     9601 agaagcagat gggggcatcg catttgatga tccagattta gcgattgatt ggccaattga
     9661 tccatcaaga gcgattatgt cggaaaaaga tcaccgtcat ccgacattaa aagaatttga
     9721 agcagaaaat ccatttgttt acggagaaat ctaagaatta aggagaaatt ttcttatgaa
     9781 aaacatcatt gttaccggag gtgccggatt catcggctct aatttcgttc attacgtggt
     9841 aaacaatcat ccagaagtgc atgtcactgt tttggataaa ctgacttatg caggaaacaa
     9901 agagaacttg gctggacttc ctagcgatcg cgtagaattg gtcgtaggag atgttgcaga
     9961 tgcagaatta gtggaccgtc tagtaaaaga agcggacgca gttgttcatt acgcggcaga
    10021 atcccataac gataattcat taaaagatcc tttcccattt gtacaaacga atatcattgg
    10081 tacgtataca cttttagaag cttgccgcaa gtatgatgta cgctatcatc acgtatctac
    10141 tgacgaggtt tacggcgatc ttccattacg tgaagacctg ccaggacatg gggaaggcga
    10201 aggagaaaaa ttcactgcgg aaacaccata taatccttca agtccatatt cttctacaaa
    10261 agctggttca gatctgttag taaaagcatg ggtgcgttct tttggcttac gtgcaaccat
    10321 ttcaaactgt tcaaacaact atggtcctta tcagcatatt gaaaaattta ttcctcgcca
    10381 agtaacgaat atcttaagcg gtattcgtcc aaaattatat ggcgaaggaa agaatgttcg
    10441 tgactggatc catacgaatg atcattcttc agcagtgtgg ctgattttga cgaaaggcag
    10501 aattggcgaa acctatctga tcggtgcaga cggtgaagaa gataataaga ccgtgatgga
    10561 attgatctta gaaatgatgg gtcaaccaaa agatgcgtac gaccatgtaa acgaccgtgc
    10621 aggacacgac ttgcgttatg caatcgattc gacaaaatta cgggaagagt taggatggaa
    10681 gcctgagttt acgaacttcc gtgatgggtt ggctgacacg atcaaatggt acgaagaaca
    10741 tgaagactgg tggaaaaaag aaaaagaggc cgttgaagca gcatatgcca aaaatggaca
    10801 ataatacaga gaaaatgtca ctgtattaat ggaaaaacac atgtgaggca ggcgctcgcc
    10861 tgtctctttt ttaaaataaa aatcaaggag cggataatca tatgttttta ttaactggag
    10921 gaaatgggca actaggtaca gaattgcgcc atttattaga tgaaaaaaat ctgcaatatg
    10981 tttcaacaga tgcaaaagac ttggatatca cagatgccga gaaaacaatg gcttacatca
    11041 cagaattaaa acctgatgtt attttccatt gtgcagctta cacagcagta gataaagcag
    11101 aagaagaagc aaaagaatta gatgagaaga tcaatgttga tggtacaaga aatgtagcac
    11161 ttgcagcaaa agctgtgaat gctcaattcg tctatatcag tacagactat gttttcgatg
    11221 gaaaaaacaa aaatgtagaa tacgaagtag acgaccaaac gaatccacta aatgaatatg
    11281 gtcgtacgaa attattaggc gaaaaagcag tagaagagat tttagaagac tattatatca
    11341 tacgaacttc ttgggttttc gggatctatg gacacaattt cgtcttcacg atgcaacgcc
    11401 tagcggaaac ccgcgacaaa ctgactgtag taaatgacca gtttgggcga ccaacttgga
    11461 cacgtacttt ggcagaattt atggtatatg tcatcgaaga aaaagcacca tttggtattt
    11521 atcatttgtc gaacgaaaat agctgttctt ggtatgaatt cgcaaaagaa atattgaaag
    11581 atacagatgt agaagttgca cctgttacgt cagaagaatt tccgcaaaaa gcaacacgtc
    11641 cgcagtattc agtcatgagc ctgaaaaaga cagaagagtt aggatttgtc ataccaactt
    11701 ggcaagaagc acttgcgcaa atgctggaag ctgccaaaaa aatgaataag taatttcctt
    11761 ttatcaaata gaatttggag catgatatgg aaaaacaaac aagaagtgca cgacgaaaag
    11821 aaaagccaaa agcgaaccag gcagattcga atttggtgct tgacctgttt ctaaaactcc
    11881 gctatcttat tggcgtttta gtcatcgtgg tcatcgttac ttttaattta aacggaagtt
    11941 cacttggtgt ttgggataaa tatgtgtcgc aaagagacga tgggaaaaaa actgatgtga
    12001 tttttggaca gaatcgggaa gtgcggtcag acgaatggct ggtgcagaca cctttttacc
    12061 tctcacaagc tgaagaggac tttccgttgg tgaacgagga ttattcatta aacggacaaa
    12121 acatgatcat tgcgtataat tctcctgtaa aggatattac ggttatcgga aaaccattca
    12181 actggggatt ctttttcctc ggaagagatc gaggactttc cttttattgg gcgatgaaat
    12241 tagtcgtcat ggtcttgctt ggattcgaag tactgatgat attaacgaag agaaaaaaag
    12301 ctctatcgct aatcggtgca tttgcgctga cattttctcc agcagtccag tggtggttca
    12361 tgcaacatgt gggcgatttg attttcttta ctttgggact gatgactgct ttttatcatt
    12421 atttctacaa tcatgataaa aaatggctac gaacgttgat gatgcttttg gtggttattt
    12481 tcggtattgg gtttatttta gtgatttacc cagcccatca agtgatgctg gcgtacttgc
    12541 tgatttttta ttttgttggc ctgcttattt actatagcaa gaaagtttca tgggatctgt
    12601 tggatgtagt gttgattgga ggggcagttt tatttattgg cggagtcatg gttcatttct
    12661 ggctaacgtc aaaagacgca ttgttagctt ctttgaatac cttatatcca ggaaaacggg
    12721 taagtactgg aggaaagtgg acgattggcg aatttttttc tttccttaca aattggaaaa
    12781 ttccttttga agatatcaac tattcgaata actctgaagt ggccctgttc tatcactttt
    12841 ttccaactgt attcctagca agtccatttg tgttatttgg caaaaaagat agcgaacaaa
    12901 agttactggg acgtattttg atgatttttt gtctgttttc tatcttttgg attaccgttg
    12961 ggttgccaaa atggctagca gaaatcactc tgctgtctta tgttcctcca ttaagagcca
    13021 tccttacttt tagttttgcg gcttgtttat tatcgatctg gtttatcgct tatatttgga
    13081 gagaagatgt tattcctatt tggtataaat tcattttgtt ggcagtaaat gcatggtctt
    13141 atttctatgc gattacgcat tcgaaaatgg ataattattt ttcggatttt gaaaccatcg
    13201 cagttgctgt gttgggaaca gtgattttag cttttgcatt atttggctgg agacgcttgt
    13261 tttacctcgc ttttgccgcc ttagttatcg tatctggctt tttcgtcaat ccagtcgtag
    13321 aaggtacagg agcgattttt gaaaaaacac tggcaaaaga aatccaaaaa atcgatcaac
    13381 aagatcctgg taaagtttgg ttgtctgaag atgaattata taacttcacc ccaacattgg
    13441 gagtgaaatc cttcaataca gtacgctttt atccagatat agctgcatgg aagaaaatcg
    13501 atccgaaaga agaatacgag aagtattata atcggtatgc tcatacaaga gcttttatcg
    13561 ctaaggaaga tacaaagttc attttagatc gtccagatat gttttcggtc acgttgaatt
    13621 ttgaagacgc agaaaaatta gggatcgact atgtgatcag taaacgacca ttaactgatt
    13681 acaaccaatt atatgaagcg cagttcacgc agctatatgg accggataaa gacggttata
    13741 tgatttttaa agtagaatat ccggattatc cagatgtacc acaaacacaa actcaggaaa
    13801 atcagctagt cgtacagtaa taggctagct gagaataatc ctgaaatgtt agtagaaagg
    13861 agagtggaga taattcaagc aatcgtacaa aaatttgtca acaaaatgag tgtcggtttc
    13921 ttgcttttgt tactgacgct agtctttttt gtttggacag cagggttagg ttttccagca
    13981 actttgttta tatataacaa tagttcatta cttatcgctt ttagtgtggc tattttgctc
    14041 attttaaatg tttataaagc aaaaggcgtc gattttcttt atctaggtgt tgcgcttgcc
    14101 cttttcattt tcttttctta ctttcgggaa ttaagaaatg agtctttatt tggagactta
    14161 ctgtttcctt tgattatttt attttgttta gtaattaaat ggatcgagtg gacaaaatgg
    14221 gatcgcggta tttatctgat tgtcttttcg gtcttcctcg gaatcacgtt atatcgtgta
    14281 tttacagaga tccaagtacc agaaggacaa agcatctggg ctccaggcaa ttcgctttct
    14341 gatatttgga tcaatacgaa tataatcgga agttctttga tgactcttgc tttattgatc
    14401 agtggatttg ccagttcttt tgaacgatgg tatgtacgaa tcatcggtgc agtagcagtt
    14461 gttgcagctt ttctcgggat ttgggtctgc caatctagag gtgcattagt cgcgttgatt
    14521 gtttttgcga tattggatct tttacctaaa tcgttgatgc gtgtcattcg tgcgccattt
    14581 attgcttaca cggttacgat tcttttagct ttgccgattt cttatctggc ggctgtctct
    14641 gaaaaagtca atttgtttac cggtcgagaa gatatttggc ataaattcta tcagacgcta
    14701 ggagaaaaat ctgaacaaat tttactagga atgaaaacat ttatcttcca acgaggaaac
    14761 caatttttag gaaaccacaa tagttataat tctattttga atatctatgg attgatagga
    14821 tttgggattg ctgctttgct gcttatcctt tttattggtc gcttaacgtt gaaagcagat
    14881 ttatccaacg ggcagatgac ttttatctgg gccttttttg cggtgatgat gcagtcattt
    14941 atggaggata cattgacttc atacccatgg gttccgatta tttatatcct gttggctatg
    15001 gccgttcatc gatatgatga accagaagtt gcggaggaat tctatgttat ggaagaggca
    15061 tatgacgata tgacttatga tgatacatct tatgaggaac aaccagatag cgctcattct
    15121 tccaggatcc aacgctatca ttgataggca caaaatacga gagaggctgt gacaaccgtc
    15181 gcagcctttt ttggtttatt gcaattgtct agcatcttct gtaaaataga aacgttagag
    15241 aaaatcttgt atcatagaaa attaagaaag aaataagaac gcagctcggt accaaaaata
    15301 tggtaaaatg gcacttgact gtaattcact gaaaatcgct tggaggaaca caaatgttta
    15361 aagatttgta tttatttatg aaggacattt atcagaacag aaaattattg ctacagtttt
    15421 cgatcaatga cttcaaatca agatatgcag gatcgttttt aggaatctta tgggcgttta
    15481 tcaatccgct atttactgta ttgatttatt ggctggtatt tggtgttgga ctgaaagcac
    15541 aaatgatcga tgcaaaatac ccatttatcg tatatttagt taccgggatg attccctggt
    15601 tcttcttctc agatgcattt gcatcaacta cggttgtttt tcgggaatat acttatttag
    15661 taaagaaagt cgtattcaat attcgtatct tgcccacgac gaaaatccta tccaatctgt
    15721 acacccactt attcttcatt ctgattggca tagctgtcgc gcttataaat ggtatatatc
    15781 cttcagcaat gtctttacag ctgatttatt atttattttg catgtgtgct ttcttgacag
    15841 gattgacttg gctgacggca tcgatccaac catttttacc tgatatcatg caattaatta
    15901 cgatcgtgct gcagttgatc atgtggacat taccgatatt atggagccct aatcagtttg
    15961 attcaggtct tgtaagcata ttgaaattaa atccgctgta ctatgttgtt caaggttatc
    16021 gtgagtcatt tttaagtgaa gcttggttct gggagcatgg aacatatact ttgtatttct
    16081 gggttttcac gttagtaatg ttcgtaatcg gctctgcagt gttccgtaga ttgaaaccac
    16141 atttttcaga tgtgctgtaa ggagaatagc tatgacagaa tatgcaatta gactaaaaga
    16201 tatcacaaaa tcttataata tgtacgcaaa accttcggat cgatttcgtg aagccctcaa
    16261 tccttttaag aaatcctatc atgatgtatt ttatgcgttg aaaaacgtga atgtagaaat
    16321 aaaaaaaggt gagatgatcg gtttcgtggg agaaaacgga tcgggaaaat ctacaatgtt
    16381 gaaaatcatt acgggagtac tgacaccaac atccggcacg atggaaatcg aaggaaaaat
    16441 ctcagctttg ctagaactag gatcaggatt caatcctgag tattctggct atgaaaatat
    16501 ctatttaaat ggaatggttc taggattttc aagagaagaa gtcgatgaaa tggtcgatga
    16561 tatcattgaa tttgctgaca tcggagacca tatttatcaa cctgtaaaaa cttattcaag
    16621 cgggatgttc gttcgtctag cttttgctgt agcaatcaat gtgaatcccg atattttgat
    16681 tgtggatgaa gcattggcag taggcgatct tgaatttcaa ctaaaatgta tggaaaaatt
    16741 tacagaaatc aagaactcgg gaaagacgat tttattcgtt tctcatgata tcagttcgat
    16801 tcgtcgtttt tgtgatcgtt cctattggtt gaaaaacgga gaagtcgtcg aatatggcga
    16861 tacgatggaa gtaacgacga attatgaaaa ctttttaaag aaaaaatcag ttaaaacagt
    16921 ggatcggaat aaaaaccaag tagaacggtt cgccgctcca gatatcgtcg acgtgacgaa
    16981 agcggaattg ttggataaag acaaaaatcc ggtggatatg ctggaacaag gagaaaaact
    17041 atacgttaaa gtaacttatg aagtaaaaga tgatacaatc aaaaatccgg ttttaggaat
    17101 tgcgctacga acagtcgaca atttttatgt atgcggactt aatactctac tagatggtat
    17161 caagattcct tggaaaaaag gagagaatat cttttatctg gagtatgaaa agttaggttt
    17221 gttagctggt gaatattatt ttgacgttgc ggtctttgaa gaaaatgcaa cggttccttt
    17281 ggtatataag acaaagtaca tgaacttgtt tgtcagcggt tcttatatcg gagaaggcat
    17341 cgtggtctta gaccataaat gggaagaagg gactcatagc aatgaaatat gattttgaaa
    17401 tggacctaga tgaacaaagc agtgtgggaa aaattgccgc acagatcaaa ccaggaagta
    17461 aagttttaga gtttggtccc ggaaacggcc gattaacgaa acatttaatc ggagcaaaac
    17521 aatgtgaagt gagcattgtt gagttagata aggaattgtt cgattttgtc agcgaatttg
    17581 ctcaagatgg attttatggt gatatcgaaa gttttgagtg ggcaaactat tatgctggac
    17641 agacatttga ttacgtcttg tttgcggatg ttttagaaca tttggtagat cctggaaaaa
    17701 cattgaagaa agtcagagaa tttttgaatg aagaaggaga gatcctgatt acattcccta
    17761 acttggcgca taattctgtc atgatcgatt tgttcaataa ccaattgccg tgggcttctt
    17821 atggtctcct agatgaaacc cataattctt tttatactca tgatggcttc caaaaagttt
    17881 ttgaaaaagc gggattattc attaatattg aagattattt gtacttagct gtaggtgata
    17941 cagaattaaa atcaacttat gaagaattgc cggaagctgt acgctatgat ttcaaaatgc
    18001 gaccatttgg tgaagtctac caatactttt tctcattgat gaaacatcca gtagcacaaa
    18061 gttccattgc agagccacaa aattctaatt atgtaaaagt gttagaagta acccagcaaa
    18121 cgaaacaaga tgaaacaatc caacaaatac cattcaataa tttcacaggg gaaaatgaga
    18181 cactaagttt ccccgtatca gaaacaacag aaagaatggt gtttcgcttc gcgcaacaac
    18241 ctagctttat cgaattcagc gcagaagctg ccggagaaaa actaacattt atcgattcga
    18301 atgctgtagt caaaacagta aatgattgtt atttgtttga tgggaaagaa ttaccagagt
    18361 tcgttctaac ggatatttct gggaaagaag tgactatcca ttgtcactat cgctttatcg
    18421 gggaattgac accaacaatg aaagaattgt tggaaacgat tcgtcccatg gctgaagtca
    18481 caaagcagct ttctgagaaa aatgacgaat tagaaagaga aaatcatcat cttcttgagg
    18541 aaaatacaag actagaccat acgttaaaaa caaccacaaa tcgctattgc acattgttgg
    18601 aaagtgatga atggacaata aaacatagac taggacgacg taagaaagaa acttcgaaaa
    18661 aaatccaaga aaaagaactt tctatatgta ttgacgagaa aatctgggat gcagaaacaa
    18721 aaatattaaa aatcatcgga tggggaatcg ctaattcgga tcgccagccc ttgtcctata
    18781 agttatcagc tgaccaatcg ccatttttcg aagcaattcc tgtttcacga gaagaagtca
    18841 atcaagcaga acagctcgca aaaggaacag aagcgggatt tgagttacga atcctttgcg
    18901 agcaagaacg ttcattctta gtggaagcag tagctcaaaa tggccaatca tggatcattg
    18961 aaatgtaaat aatgattaac ttataaagat aaggagtaaa cgaatcgtgc aaaaaaaaga
    19021 aattgccgta tttatcgatc aaatcactcg tgaacgggcc actggcgatt tgcttattgt
    19081 gggctgggca attgatgaag tgaccaaaga aatccctacg ataaaggtag aaaaagaaaa
    19141 tgtgattgct gaagcaacac atgtcgtaag attggatatc aatcatttat ataatcttga
    19201 tgttaagacc caaagcggtt ttaaggtacg tttatcaggg aaaatgagag gaaaagccat
    19261 cttagacttc caaacagcga gacatcaaaa tggcatagct gtgaaattaa acggtaaata
    19321 cccttatgat gacggcattg aatcttcatg ggaaagaaaa aaaagattac tgaaaaaagg
    19381 gataaattat gcaaggactc atggcgtaaa aaaagcgatc cgtcgggtca aattggaatt
    19441 gaagccagga tcgatcgact atgcagagtg gatcagtcgt catgagaaac cagatttgaa
    19501 agagcaaagg aaagaagcac aaggatttgc gtaccgtcca ttgatttcca tacttatgcc
    19561 tgtctataac gttgaaatca aatggctcga aaaatgcatt gattcggtac tggatcaaac
    19621 gtatgatcat tgggaactat gtatcagtga tgatgcttct actgatccag ctatccgaaa
    19681 gtgtctggaa tcctatcaag caaaagatga tagaatcaaa gtcgttttcc gaaaagaaaa
    19741 tggacacatc agtctggcaa ctaattctgc attggaaata gcagaaggag aatttatagc
    19801 actcttagat aatgatgatg aattaccgcc atttgcatta tatgaagtgg caaaagtgct
    19861 aaacgtacat ccagaactgg atgtgatcta cagtgacgaa gataaaatcg atgcagatgg
    19921 caatcgcttt gatccgcatt tcaaagcaga ctggtcgcct gatacattga tgggaaataa
    19981 ttatatctct cacttaggcg tatatcgaac aagcattgtt aaagaacttg gcggtttccg
    20041 taaaggctat gaaggttctc aagattatga tttggtcctt cgtgtgacag aacaaatccc
    20101 tgcagatcat atttaccaca ttgaccgagt cttgtatcat tggcggacga tcccaggatc
    20161 aactgcaagt aatggcgaag ccaaaagtta tatctatgat tccggagtga aagcattgac
    20221 agatgccctg tctagaagaa acatcaaagg aagcgtacgt cctggcagaa tttctggatt
    20281 ttatgagatt gcttatgacg tcttgcaaga ggatttggtc agtgtgatca ttccaacgaa
    20341 gaatggctat gaagatctaa aaacttgtgt ggattcgatc atcgaaaaaa cgtcttattc
    20401 aaattatgaa atcattattg cggataacgg aagtacagat ccaaaaatgc aagaattatt
    20461 tgcagaatac aagcatcagc tgaaagaccg ctttatcgta gaattgattg acattccatt
    20521 caattattct cgaattaaca atttagcagc agaaaaagca aacggcaaat actttttatt
    20581 cttgaataac gatacagaag tgattgagcc agattggatg acggtgatgg tttcttatgc
    20641 acagtttgat cgaatcggct gtgtaggagc aaaactgttt tatccagatg atacaacgca
    20701 gcatgcagga gtattattag gaatcggtgg tgttgcaggg catgcattga ataactatga
    20761 tcgaacacat tgtggttatt tcggccgatt ggtcattgac gtcaattact tagcagtcac
    20821 tgcagcttgt atgatggtca aggcagctga cttcaatgct gttaacgggt ttgatgaaac
    20881 attagaagta gcattcaacg atgttgactt gtgcttgaaa gtttatgaac tgggtcgtta
    20941 caatgtctat gcacatcaag cagaattgta ccatttcgaa tctaaatcaa gaggttatga
    21001 agatactcca gaaaaacaaa gacgttttgc tggtgaaatc aaaaaaatgc aagataaatg
    21061 gccagcatat attgcgcatg atccatttta caatgataat ttaacaaaag aaggaatcgg
    21121 cgacttttcg ttgagaccag attaaactat aataaaaagc tgttatgttg tgattcgctc
    21181 gcacataacg gctttttata gttttttgtc aaaaaaaggc agaaggtgcc gactcaaaaa
    21241 aataagtcga ggttgggaca gaagtgttta actccgagaa ataagaagaa attcacgaaa
    21301 attgcttttc aaatttttgt gaatttcggc ttatttaaga agaagttgct tctgctccca
    21361 ccgtttattc gtttttaaag aatgagacaa aagtgtttct tacttttgtc ccacgctctt
    21421 attcatgctc agagcgccaa tagatacaaa atcatagcca aagtttcacg aaccctagct
    21481 gtcaaagaat cgatatttta ttttaatata ggctatttta aaataaatgg tttaaggtat
    21541 tcctctacaa aagccactct atcaattatc atgggtttaa aataagaagt gatggagata
    21601 accagattgt tcttagcgtc aatataaaga acatttccac tattaccaat tgctgaatat
    21661 atttcgttct cagaatctaa tacccaccat aaatagccat atctcatatt ttgtaagtct
    21721 tctaaagcac tggttctagg tttgaacatc tcgtttatcc acatgggtgg tacgatttgt
    21781 tttcctttat acatcccttt atttaggcat aatacaccaa tttttgccag atcacttgcc
    21841 gataaacaca aaccatatcc tgccgtcccg atatttagtg gatcacaaaa ccagacattg
    21901 gttttaggag acttatccat tgtaaactgc ttatgttctt cagctgtttc agcataataa
    21961 ttgacatggt ttggtatccc tataggatca aatagatact tattggcaaa atcaacagtt
    22021 gttagcttac ttgtctgata caacaagcca gttaatatgt gtaaacagac agtttgatag
    22081 ttgaattcgc cggttatacc ttttcttcct cccaataaat ctagtgatgt ttttgtccaa
    22141 tcttcacttg aacagacttt tgtccatgga tcgcctttac atttgtaggg tgctttcatt
    22201 gttagcaagt gcttgattgt aatagcgtaa atagttttct ccccgcgttt cactttatac
    22261 tctggaaaat aatctaatac tttatcatcg atacttttga ttaatccttg ctctaaagca
    22321 atgccaatca gcaaagatac aatacttttc gttgctgaca tgatatgaca agtatcgtct
    22381 tttttataac cattccattc atctgtataa acttctaccc catccttata tgcgacaatt
    22441 tgacatatat ttggttgtcg aaaaccaata aaatcatgca actcttgttt attcataaga
    22501 tattacctca ctttcttcta taagcataat gtcattaaaa atcgatttca attttttcga
    22561 ttcttcacgt aatacaggta ttcttatgga ctagacagcg ctttgggaag atggattctc
    22621 tgaaaaatat aaaggttcac tttatgagtg gataaaaaaa gcagcaaata cttcgacttc
    22681 tttaatttac caacattagt gaaaatcgat tcatctggag atttgtaatt tagagtcctt
    22741 gtcttgatta gaggtgtata aagcttttta ttagacattt ttaaattcca ttacaatttg
    22801 cttataaact aggtttttaa aggtgactat ggtaagataa tagagatatt attttgtgat
    22861 taggagtgta tatatgaata aaaatggaga atggagtgct gcacgacgga tattcattat
    22921 attaatggat gttattgtat acaatgcagc aatatatagt agtttcttac tgaagtttcg
    22981 tggagagatc ccattacgaa atttagaaac tttccaacac tcagcaatta ttatttcagt
    23041 gatttttatt gcgttgaaca ttttgttggg tgcatatgtt ttttataatc gaatgatcag
    23101 tgacatcgtg tttgtcacag tcatcggtca ggtattgatg acactaggga tcatggtagt
    23161 gacttttgcg ggccgctggt ttgcttttcc acgtcttgtt atcttacttt catttgttct
    23221 cggtacgctt ttcttaagta tctatcgtat tctcgtatat aaactttatt tacgcgtaag
    23281 tcgagataaa aaggtcgtca ttattgggtt agaaaaggat gtcgcaccag caatccaaaa
    23341 ttttggttca aagaaaaaca acaaacataa agtcgaagca gtcattcttg ataactatta
    23401 tgcaaacatc aaaaatatga ttaaagacat cgatattgtc tatcttgctt ctcatatcga
    23461 agaaactgaa aaaatcaaaa tctatcaatt agttactaaa aatgaaaaaa aattgttttt
    23521 gaatacaaca tttgaaaact tgacattgat caatccaaat attatgaatt tcgaagatga
    23581 aagtattatt gaagcatctg gatttagaat ccaacctgaa tatgaactat tcaaacgttt
    23641 gtttgatatc atcgtttcac ttgttttatt agttattgct tcaccattta tgttattcac
    23701 agcaatttta gtcaaagtga catcaccggg accaatcatc tataaacaag tgcggatcac
    23761 aaaaaatcaa aaggaatttt caatctataa attccgctcg atgaccgcca cagccgaagc
    23821 aaaatctgga ccagttttag ctaagagcaa tgatgcacga gtaacgcctg taggtaaatt
    23881 tattcgagct gtccgctttg atgaattacc acaaatcttc aatgtgctga aaggcgatat
    23941 gtcgatcgta ggaccacgtc ctgaacgtcc tttcttcgtc gatcagttca atgcagaaaa
    24001 cccttattac tacttacggc ataatgttcg tgctggtatc actgggtatg cacaagtcta
    24061 tggaaaatat gtctctgatt acaacagtaa attacgtttt gatttacttt atatcaaaaa
    24121 atattcattg atgcttgatt taaagatctt gctgcaaacc atcaagatcc tatttgataa
    24181 aatgtcctct caaggacttg aagaagacga aaatgtgatc tcattggcag aaattgcttt
    24241 tccgaatgaa aaaatctttc gctagttggg aggcgtaaaa atgaacaatc cgaaattcag
    24301 cataatcgta ccagtataca atgcagaagc aactattggt cgaacgattg atattctaaa
    24361 agaattggat tatgaagagt acgaagtcgt tttagtaaat gatggatcaa cagatcaaac
    24421 acaggaaatc attcaagaac tgattgaaaa tgatgaacgt tttgaattga ttactacgac
    24481 caatcaaggg cccggattag caagaaacga aggaataaga cagtcaaaag gagaatatct
    24541 cttattcttc gatgcagatg acacgccagt aaaaacgatc ttgtcagatt atgatcattt
    24601 gctagaaaag agtccaaaag cggatcttgt tgttagttcg tttgtttttc gtacaatgga
    24661 cgagggaaga atcgtttccg aaaaagaaaa cttagttaca gaatgtcact atctaactca
    24721 cgaagagttt ataaaagaca tgtatcagtt aatgaatgaa cagttgatgt atgttgtatg
    24781 gaacaaatgc tatcgtagcg atattctgaa aaataaccaa attctattta aaacttataa
    24841 tagttgtgag gatcgaatat tt
//
LOCUS       DAZKLM010000036        24258 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3764
            SAMEA115706913-rid22582563.denovo.036, whole genome shotgun
            sequence.
ACCESSION   DAZKLM010000036 DAZKLM010000000
VERSION     DAZKLM010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706913
            Sequence Read Archive: ERR13196647
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24258)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24258)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287823.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287823.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,004
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,849
            CDSs (with protein)               :: 2,849
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 41 of 96
            Pseudo Genes (incomplete)         :: 52 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 15 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24258
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706913-rid22582563.denovo.036"
                     /isolate="VRE3764"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(45..1340)
                     /gene="purB"
                     /locus_tag="ACG842_001336"
     CDS             complement(45..1340)
                     /gene="purB"
                     /locus_tag="ACG842_001336"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HFQ0247889.1"
                     /translation="MLERYTRPEMGNIWTEENKYRAWLEVEILADEAWAELGEIPQED
                     VKKIREHASFDIDRILEIEKLTRHDVVAFTRAVSETLGEERKWVHYGLTSTDVVDTAY
                     GYLIKQANDILREDLKRFTEIIGEKAKEHKYTVMMGRTHGVHAEPTTFGLKLATWYSE
                     MKRNIERFEHAAKGVEAGKISGAVGTFANIPPFIEEYVCKQLGIRPQEISTQVLPRDL
                     HAEYFSAMALIATSIERFATEIRGLQKSETREVEEFFAKGQKGSSAMPHKRNPIGSEN
                     MAGLARVVRGHMVTAYENVTLWHERDISHSSAERIILPDTTILLNYMLNRFGNIVKNL
                     TVFPENMKRNMSATFGLIYSQRVMLKLIDKGMTREEAYDLVQPKTAYAWDNQVQFRPL
                     LEEDPTITSVLSKEEIDDAFDYHHHLKNVDVIFKRVGLE"
     gene            complement(1353..2477)
                     /gene="purK"
                     /locus_tag="ACG842_001337"
     CDS             complement(1353..2477)
                     /gene="purK"
                     /locus_tag="ACG842_001337"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HFQ0247890.1"
                     /translation="MVKPLLPGSTIGIVGGGQLGRMMAISAKEMGFKVGVLDPVSDCP
                     AAQVADWHIESVYDDTFALKELARRTDVITYEFENVSVEALNAILPMSFIPQGTDLLA
                     ITQDRLLEKSFLETNNIVIAPYATIVSPTDIQDAIDGIGYPCVLKTTRGGYDGKGQYV
                     LKSRADLAPAMDLLREGTCVLEAWIPFEKEISIMVAGNGQGDFTTFPVVENIHHNNIL
                     HETIAPAAIDQDVIEEAERIARVIAEAVSLSGVLGVEMFLTKTGGLYVNELAPRPHNS
                     GHYSIEACSMSQFDAHIRGICGWPLGEKVRLISNAVMVNVLGEQLLESYRLIENKPDW
                     QFHFYGKKQAKKGRKMGHITILTEDIQQTLEEIKQTNIWD"
     gene            complement(2470..2958)
                     /gene="purE"
                     /locus_tag="ACG842_001338"
     CDS             complement(2470..2958)
                     /gene="purE"
                     /locus_tag="ACG842_001338"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HFQ0247891.1"
                     /translation="MTAKISVIMGSTSDWETMKEACQILDEFGVSYEKKVVSAHRTPD
                     LMFEYAQNARKKGIKIIIAGAGGAAHLPGMVASKTTLPVIGVPVQSRTLNGLDSLLSI
                     VQMPGGVPVATTAIGKAGAINAGLLAIQMLSMYDLELEAKLAERRSMLAKTVIESSDQ
                     LG"
     gene            complement(3374..4681)
                     /locus_tag="ACG842_001339"
     CDS             complement(3374..4681)
                     /locus_tag="ACG842_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleobase:cation symporter-2 family protein"
                     /protein_id="HFQ0247892.1"
                     /translation="MEKETQNGKAAVLGLQHLLAMYAGAVAVPLLIGTGLGFNQEQMT
                     YLISIDIFMCGLATLLQLTVNRFFGIGLPVVLGCAIQAVAPLILIGTNEGVGAIYGSI
                     IASGIFVVLVSGVFSKIKKLFPPIVTGTVITVIGLTLIPVAIEKMGGGNATAADFGDK
                     TNLLLAFVTILLIVGVQMLAKGFIRSIAVLIGLVGGSILAAFMGMIDLSAIGDAPLFH
                     VPQPFYFGKPTFDVWSILLMIIISMVSMVESTGVYFALGDITGKNVGEEELKKGYRAE
                     GLAVILGGIFNTFPYTGFSQNVGLVQLSGIKTRKPIYFSAFFLIVLGLFPKIGALAQI
                     IPEPVLGGGMLVMFGMVAVQGIRMLLEVDFSNDKNLLIVAVSVGFGLGFNVTPTLFSQ
                     MPQTLQMFTGNGIVMSSITAIILNLIFNGLKKDEEILEEAFKK"
     gene            complement(4684..5265)
                     /locus_tag="ACG842_001340"
     CDS             complement(4684..5265)
                     /locus_tag="ACG842_001340"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0043101 - purine-containing compound
                     salvage [Evidence IEA];
                     GO_process: GO:0046110 - xanthine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HFQ0247893.1"
                     /translation="MKELVERIQKDGRILGEGVLKVDSFVTHQVDPVLMEQMGKRFAE
                     VFKEQGITKVVTIEASGIAPALFAARELDVPMIFARKAKSLTMDEELLTASVYSFTKQ
                     VTSTISISRKFLSSEDKVLIIDDFLANGQAAKGLIELCQQAGAQVEGIGIVIEKSFQD
                     GRQLLEEMGLRVVSLARIASLTNGQVEFLEEDA"
     regulatory      complement(5381..5478)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            complement(5837..7129)
                     /locus_tag="ACG842_001341"
     CDS             complement(5837..7129)
                     /locus_tag="ACG842_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFQ0247894.1"
                     /translation="MKKKYVQVGLGGRARFFYEAIASTYHQTCELVGFCDVSQTRMDY
                     ANRILVNKYGQKKVKTCMPSQFEQLLDDVRPAEVIITSVDRTHHDYIIRAMKKGYDVI
                     CEKPLTIDEEKAQEIITVQKQTGRSLRVTFNYRYAPYHTKIKELIESDVIGDVFSIHF
                     EWLLNTQHGADYFRRWHRNKHNSGGLLVHKSTHHFDLVNFWLNSRPQKVFAFGGLNFY
                     GMHNAEKRGVEIFYSRCYGSELAKADPFAIDLSANEELREMYLEAEKDSGYIRDRSVF
                     SDDISIEDTLGVTVKYETGAILTYSLNAYMPWEGFNVSFNGSKGRIEMKVIEKSYINA
                     GGDKKDEGAAVLQSIHVYPMFGEPYEVIVEQEAGGHGGGDPRLLDDLFGEEKEEDPWN
                     RAATHVDGILSILTGIAANHSIASGKAVVIDELVSLKE"
     gene            complement(7126..7749)
                     /locus_tag="ACG842_001342"
     CDS             complement(7126..7749)
                     /locus_tag="ACG842_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0247895.1"
                     /translation="MKKPIEEMFLYKLNYYVCRIFLTSLISNALFALVLGCLFTLGLV
                     LSARVVYLIYFLMMYPSYYLSVHYLAEKKVCWRKGKIKRMCFIITLFQLFVIIFAVDW
                     YLLVGKIGAWYLIPFLLCLLLFVSHSFFSTLIDICKEDKADSLVYLIKRNIWLLSKNV
                     KGFLWYLCLLYGLFWCIQHDFLLTALLYPGLPFVTVFCLKEKENDFK"
     gene            complement(7752..8588)
                     /locus_tag="ACG842_001343"
     CDS             complement(7752..8588)
                     /locus_tag="ACG842_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0247896.1"
                     /translation="MNNTVTKWFQYGFILLLGLVMIYPVLWMIAGSFKTDQEIMSGSL
                     NLIPETFQWENYAKGWAGFAGISFFTFFRNSFVISAVSTIGTVLSSTCIAYALSRIDF
                     KGKRFWFVVMLVTMMIPAQVILIPQFIIYNRLNLVGTYVPLILPHFFGQAFFIYQIMQ
                     FMVSIPKELDESAIIDGCSKYSVFTKIIFPLLKPSIITTIIIQFYWKWDDFMGPLIYL
                     NKPRSYTVSIAIKLFADAGSSTTYASMFAMSTLSLLPVFLIFLFFNKYLVQGISTSGL
                     KG"
     gene            complement(8605..9492)
                     /locus_tag="ACG842_001344"
     CDS             complement(8605..9492)
                     /locus_tag="ACG842_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0247897.1"
                     /translation="MKKEKLKRFLDQDNVVGYVFASPFILGFLLITVIPMILSLYYSF
                     TDYRLGNPVSWIGLDNYIKMLSDARFLNSIRVTFLYVFASVPVKLIFGLLIAYLLTRN
                     IKGTTFFRSLYYVPSLIGGSISIALVWRELFSKNGLVNIFLERLGAPALSWFSDQKLV
                     MIPLVLMSAWQFGSAMIIFAAGIKEIPGSYYEAAEIDGANKWQTFYKITLPLLSPIIL
                     YNLVMQTIAAFMTFTQAFIITQGGPNDATNFYALYVYNQAFSFNSMGYASAISWIMLL
                     IMGAVTFLIFKSSKKWVVV"
     gene            complement(9508..10791)
                     /locus_tag="ACG842_001345"
     CDS             complement(9508..10791)
                     /locus_tag="ACG842_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFQ0247898.1"
                     /translation="MKKRMRIILALSVMLFFLTACGQEDDKKITLNMAWWGSQVRHDA
                     TVKVIELYEEKNPHVTIDYEFYDYEGYYTKLGTLAASDDIWDIFQLNNQFPQYINQIE
                     PLDEYVEQGIIDISNADESYLATTTYDGQLVGLSNGVNSFAIAYNKNIFEQLNIPEPA
                     SNWTWDEFEQISYQITEELGIYAISRFEDFIAGCIIQIPQVEKGLNFYNREDLSNSLG
                     FENPDYLTDFFQLRKNLVDKGAHPEPGGGAASTTDLSFQAVRDREAAMVFLSSNQLTE
                     ILSGAPEDMEIQLINPPRRKADGESGIPLRSSQMLSMAKSSAHKEEAAKFIDFFQNSE
                     EANEILKGERGVPIMSNIREQLARADDATLQATFDYLEMISDMDNGEVNVFDSALNPE
                     IQDQYNLLVEKVIYEEVTAEEAAESLYEFASNILG"
     gene            complement(11474..12157)
                     /locus_tag="ACG842_001346"
     CDS             complement(11474..12157)
                     /locus_tag="ACG842_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HFQ0247899.1"
                     /translation="MAEQFTPEIMTELRKDIVRVPEIILEASGIKIFGKLIRSIIFTT
                     DIAIIRNTNANAVIAVYPFTPHPAITKSIIEAADIPVFSGVGGGLTQGARSSYMSMFA
                     EAQGSLGVVLNGPTPLETVKEVCRVVDIPVISTVTSKYTEIDEKLAAGISIINISAGK
                     ETAEAVRHFRKKYPELPIIATGGPTEESIAETIQAGANAITYTPPSSGELFSRKMEKY
                     RETEKKNHE"
     gene            complement(12175..12792)
                     /locus_tag="ACG842_001347"
     CDS             complement(12175..12792)
                     /locus_tag="ACG842_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HFQ0247900.1"
                     /translation="MAKKKWKKQKRKNIRWPAVVAGVAVILLAFVFSLKNLSSPFTDN
                     QQENEAQSHQQFIERLVPHAQELQDGYGVLPSIILGQAILESNWGKSTLASKYNNLFG
                     IKAYGDQKKVSLETKEFVNEEWITIQGDFKVYDSWEQSMDDHTQLFVQGVDWNPALYE
                     KVITATNYQEAAQALQDAGYATDPGYAQKIIQVIETYQLNQYDRR"
     gene            complement(12802..13626)
                     /locus_tag="ACG842_001348"
     CDS             complement(12802..13626)
                     /locus_tag="ACG842_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HFQ0247901.1"
                     /translation="MNKFLGFGAVVIMIAAMGYVIFSDDDSHVQAGNQAVERTSTKTT
                     ASKEKNSQTTSSKVKDKETKDRTSKQLPDVKTDDWKLVLVGPDHKLEKEIDESTQLAS
                     LSNGYLIDKRIAPEYEAFAKAAEAAGFPLVMVSAYRSVSSQQQVFEQNVQDVMSRQGV
                     SEEEATKITKETITEPGYSEHHTGLAVDVVDQNWYNSYPSTVLDASYGDQPGAKWIAD
                     NAAQYGFIVRYPKGHEDITKITYEPWHLRYVGKENAEYITKHQLTLEEYLNELNEK"
     gene            complement(13619..14179)
                     /locus_tag="ACG842_001349"
     CDS             complement(13619..14179)
                     /locus_tag="ACG842_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0247902.1"
                     /translation="MHQVYIDLIMSVLLEQFETEKNFLDEYLLISPEQWQKWKDGEKN
                     LPSEAMQKVKNLFTDYEWMLTQKILRQTIIFPEKRNVAVAEYKQIKTRIAQKWLSSKA
                     AKVELIPLKNNQQAGRYLDLKVSIQYDEWGYDDILNFRLPADVQKQIEGEKIELLDWV
                     NENLEETYVHEANGEREEQRKDFSSE"
     gene            14353..15309
                     /locus_tag="ACG842_001350"
     CDS             14353..15309
                     /locus_tag="ACG842_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFQ0247903.1"
                     /translation="MKKYQWGIVGLGTIAHEFAESFNQETSELTAVASRTSEKAENFA
                     HRYNIPKAYGSYQEMLDDAEIDIVYIAVPNRQHIDHILAALEAGKHVLCEKAITMNKK
                     ELADAMRLAEEKNLILAEAMTIFNMPLYQQLRSIMDTGKLGALKMIQAPFGSYKEPDP
                     KNRFFNPELAGGALLDIGTYAVSFARFFLSSQPEVVASTMVPFETGVDEQSVTILRNK
                     ENELAAVSLTFQAKMPKVGIVAFENGYVTIADYPRADRAEILFTDGTKEFIESGNTSE
                     AMNYEIKNMVHMIEGDLPNRSLFLTKDVIEILDQMQVLWKNM"
     gene            complement(15614..16570)
                     /locus_tag="ACG842_001351"
     CDS             complement(15614..16570)
                     /locus_tag="ACG842_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HFQ0247904.1"
                     /translation="MRIGMRTVKTVISVFFSMVTAGSLSLLYWPAAGIIALLSVGNTK
                     RSTLRTGMNRIAAFILATATALFSFFAVGYTIFGFSLFLFLFIPLASRFKLTEGIVVN
                     SVLVTHYLVEENMSWQLIGNEAGLMAIGLVFALLANVYMPDRTKQLKENQIQIEKEFR
                     NILRQMAEFLLSENKEDVQIKCEHLLTFIRESQEDAREHQENYWLRQPLYYETYFSMR
                     RAQANVIKDMLENLERIQQPAYYGKHIYGLLIYTAETFSESNDGRQILTRIEEVYVLY
                     RQMPLPTSRPEFEDRAELFQFLQSFKSFIEIKAEFSQQKIQK"
     gene            16674..17276
                     /locus_tag="ACG842_001352"
     CDS             16674..17276
                     /locus_tag="ACG842_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFQ0247905.1"
                     /translation="MQFGRFRLGMRTIKSALAVFLCILFFHLTQRGIPMIAALSAVFS
                     LRQDLTTTVSFGKSRIIGNTLGGTLAIIYFFVKSYFHNDFLVELILLPILVIIVIVVS
                     DGINNNSGIISAIATLLLIALSIPQGESALYAIQRVLDTFIGTFIAIGINFFLRPPET
                     EKKEELSEDLVELQKKERLLRTNLQEIEAKIQKQKKNSED"
     gene            complement(17364..18863)
                     /locus_tag="ACG842_001353"
     CDS             complement(17364..18863)
                     /locus_tag="ACG842_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HFQ0247906.1"
                     /translation="MEIPVVFIAIAIAALILLMLLIVFVAKYQTAKPDEALIISGSYL
                     GSKNVHIDESNNKIKIVRGGGAFVLPVFQRSNRISLLSSKLDVSTPEVYTEQGVPVMC
                     DGTSIIKIGSSVEEIATAAEQFLGKTREELENEAREVLEGHLRSILGSMTVEEIYQNR
                     DKFSQSVQEVASVDLAKMGLIIVSFTIKEVRDKNGYLDSLGKPRIAQVKRDADIAEAE
                     ALKETRIKKAQAEKESQAAELQRQTEIAESLKEKELKLATYKQEQDVAKAKADQAYNL
                     ESARAQQQVIEQEMQIKIVERQKQIELEEKEITRREKQYDSEVKKKADADRYAKEQEA
                     QAQKVKEVTEAEAERFRVEALAEAEANKTRLAGQAEAEAALAKGKAEAEAKQKIANAF
                     KEYGEAAVLSMVIDMLPQLMREAAQPLGNIEKISVVDTGSSSGETSGANRVTNYATNL
                     LSSTQETLKATTGLDLKEIVENLSTRGTTSQVNMYEAPKEAEQNSMKEE"
     gene            complement(18879..19403)
                     /locus_tag="ACG842_001354"
     CDS             complement(18879..19403)
                     /locus_tag="ACG842_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HFQ0247907.1"
                     /translation="MIGGIALETIYWYTLLICAGLAVLLIIFGDVFDFDGPLDPMLII
                     PWLTFTSLFGFLGERLTDQSSLFLFIASGGISIVLVFLLNFYILMPMKHAESTMSSSE
                     KTLEGQVAVVITPIPVRGMGEIQLKSVTGSISRPACFYSPQEQTAERGTKVLIIEIKE
                     RICYVMPYESMIKA"
     gene            19701..20450
                     /locus_tag="ACG842_001355"
     CDS             19701..20450
                     /locus_tag="ACG842_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HFQ0247908.1"
                     /translation="MNRSSVSDKISSIAKIVANDKLIQKKLQTGDPTHAIQGITNEIM
                     IASNLDFIVVMDPEGIRYSHADPSYIGLPFSSHEDVKKVIGGQEFFSEKEGMLGFGFR
                     YFVPIYYDQEIIGAVCVGLTNNTIDNQVKDGQKMIVFSLIIGLSVGIIGAVFLAQKMK
                     KVLLGLEPQEIAEQLSEKTIIENEVDEGILAISSDKTILLINTPAKNIFYQTEQLLDF
                     EVGKKSVIIYMTFCLKRRLSIKRPFGLNPCI"
     gene            20441..21226
                     /locus_tag="ACG842_001356"
     CDS             20441..21226
                     /locus_tag="ACG842_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFQ0247909.1"
                     /translation="MYLNGIETISSVTPLFSNDRFLGAVATLRNRSEVMKLMEELGGT
                     KQYINSMRAQTHEFMNKLHVISGLIDLKKYEEVTHFIQQLNQNYKKEIGQVSSLIKVP
                     AIAGFLLGKINEAKEQSVEMLLESDSFIPKMEMDDSIHSLLQVLGNLLDNAKEAVLQK
                     HERNGQVRIHLNYDTEAEVFIVTVCDDGVGISEEISEKIFTLGFSTKGINRGFGLNLI
                     HSIVKRHGGFLEFSKQTNGGTIVYLELPKKEDQNDDKCPDNRR"
     gene            21195..21911
                     /locus_tag="ACG842_001357"
     CDS             21195..21911
                     /locus_tag="ACG842_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFQ0247910.1"
                     /translation="MMTNVLIIEDDPMVAELNRTFVESIPNMNVIESFRNVEEGKKYF
                     RQKNADLLLLDVYMPGESGIHFLSWLRQQGNDIPVILITAADDIETIKNVIRYGALDY
                     LIKPFTLQRFQLAIEKYLAFSQTIQTTEKADQKLIDQVFNHTTPIHEIPSFIDLPKGL
                     SKLTLKKISAFILKEDTIFSTKELAQKSSLSRISVKKYLNFLVEQDILNERIKYLEIG
                     RPLTCYELTKNGRKKLTDYC"
     gene            complement(22013..23032)
                     /locus_tag="ACG842_001358"
     CDS             complement(22013..23032)
                     /locus_tag="ACG842_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacid dehydrogenase"
                     /protein_id="HFQ0247911.1"
                     /translation="MRIACYGVRPNEIDFFEKLNIYHYDLSLYEELLTHDNIVSAKDH
                     DAVLLRGNCIADETNLAKMQEYGIRYVFTRTVGVNHIDLQAAADFGMTVARVPSYSPN
                     AIAELSLTFAMMLLRNTAYTTIRTSFKDFRVDEQMFSREIRNCTVGIIGTGRIGLTEA
                     VLFKGLGAKVIGYDIHQTEAAKKVLTFHSLEELLAKSDIVSIHVPHIPGENDQMINEG
                     FLAQMKKGSILINTARGELQDNEAILRALKSNHLAGFATDVFANETEVFFRSFKPWET
                     IPDPAIQQLVEMYPRVLVTPHVGSNTDEALSNMISTSFENFREIIETGKTKNEVSLPK
                     ARQLR"
     gene            complement(23051..24052)
                     /locus_tag="ACG842_001359"
     CDS             complement(23051..24052)
                     /locus_tag="ACG842_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HFQ0247912.1"
                     /translation="MTSTVFIILLGFVCRKKGIFSESVGKILSKVVLTVALPALAFRA
                     FMQDINTELLMQGINVLIWGLVIYIILILVSKPFFLSYKGDKQTTLRVLTIFGSTTFF
                     GTPIVSAIYGPVGVMYSSIFNIGYRIFLYSYGYIKMSGLKMEAKNIKAMFVNPIIIAT
                     FAGLFIWLFQGYLPQVSVTGAEGEISQVAFLRIDQTAPWLFQSMDYLAGLSSPLAWLA
                     IGATLGEVSFKKAASEKTSWYYSLVKVVFVPALNIVILAVLTATNILPVNFEALATIV
                     IMMATPAATVAAAYAISFDREALLASNASLLSTIFAVVLTPVWIVVLQMIHGIGLFS"
BASE COUNT         7189 a         5221 c         4224 g         7624 t
ORIGIN      
        1 tttttgctat tctattttaa ttgaattttg caactcactc ttctttattc caaacctact
       61 cgtttgaata tcacgtcaac gtttttcaaa tgatgatgat aatcaaaggc atcgtctatt
      121 tcttctttag ataataccga tgtgatggtt ggatcttctt ctaacagcgg gcggaactgc
      181 acttggttgt cccaagcgta cgcagttttt ggctgaacaa gatcataagc ttcttctctt
      241 gtcattccct tatcgatcaa tttcagcata acgcgctggc tataaatcaa accaaatgtt
      301 gcagacatat tccgcttcat gttttcagga aagaccgtta gatttttaac gatattgcca
      361 aaacgattta acatgtaatt caataaaatc gtggtatcag gcaagatgat tcgttctgct
      421 gaagaatgag aaatatctct ttcatgccat aatgtgacat tttcataggc agtgaccatg
      481 tgtccgcgca ccactctggc tagaccagcc atattttctg aaccgatcgg attccttttg
      541 tgcggcatag cagaagaacc tttttgacct ttcgcaaaaa attcttctac ttcacgtgtt
      601 tcggattttt gcaatccgcg tatctctgtt gcaaaacgtt caatacttgt agcaataagc
      661 gccatcgctg agaaatattc agcatgtaga tcccgaggga gaacttgagt agagatttct
      721 tgtggacgga tccccaattg tttacataca tactcttcaa taaatggcgg gatatttgcg
      781 aacgttccca ctgctcctga gatttttcct gcttctactc cttttgccgc atgttcaaat
      841 cgttcgatgt tccgtttcat ttctgaatac catgtggcaa gtttcaatcc aaaagtagtt
      901 ggttcagcat ggacaccgtg tgtccgaccc atcatcactg tatatttgtg ttcttttgct
      961 ttttcgccaa tgatttccgt aaaacgtttc aagtcttccc gtaaaatatc atttgcttgc
     1021 ttgatgagat agccataagc agtatctacg acatccgtac ttgttagacc ataatggacc
     1081 cattttcttt cttctcccaa tgtttcagaa accgctcgtg taaacgcaac gacatcatgg
     1141 cgtgttaatt tttcgatttc caagatccga tcgatatcaa atgatgcatg ctcacgaatt
     1201 tttttcacat cttcttgcgg gatttcacca agttctgccc aagcttcatc tgctaaaatc
     1261 tcgacttcga gccacgcacg gtatttgttc tcttccgtcc agatattccc catttcaggg
     1321 cgtgtataac gttctaacat gttttttctc ctctaatccc aaatattcgt ttgtttgatt
     1381 tcttccagcg tttgctggat gtcttctgtc aagatagtga tatgtcccat cttgcgacct
     1441 ttttttgctt gtttttttcc atagaaatgg aattgccagt ctggcttatt ctcaatcaag
     1501 cgatagcttt ccagcagttg ttctcccaaa acattgacca ttactgcatt gcttatcaaa
     1561 cgtacttttt ctcctaatgg ccaaccacaa atacctcgaa tatgggcatc gaactgactc
     1621 atagaacaag cttcaataga ataatgtcca gaattgtgag gacgaggagc taattcattc
     1681 acatatagcc cgcctgtttt tgttaaaaac atttctaccc ctaaaacacc tgaaaggctc
     1741 actgcttctg caatcacacg ggcaatacgc tcagcctcct cgatcacatc ctgatcaatc
     1801 gcggctggag caatcgtttc atgaagaata ttgttgtggt ggatgttttc cacgaccgga
     1861 aaagtcgtga aatctccttg accatttcct gccaccatga tcgaaatctc tttttcaaac
     1921 gggatccatg cttctaaaac acaggttcct tctcttaaaa ggtccatggc aggagctaga
     1981 tccgcccgac ttttcaaaac atactgcccc ttgccatcat agccgcctcg agtggttttc
     2041 aaaacacaag gatagccgat cccgtcaatc gcatcttgga tatccgtcgg actgacgatc
     2101 gttgcataag gagcaatcac gatgttattc gtttccaaaa aagatttttc taacaggcgg
     2161 tcttgcgtaa tcgcaagcaa gtctgtccct tgaggaataa atgacatggg taaaatcgca
     2221 tttaacgcct ctacactgac gttttcgaat tcataggtaa tgacatctgt tcttctggca
     2281 agctctttca gcgcaaaggt gtcatcatag acactttcaa tgtgccaatc tgcaacttgt
     2341 gcggctggac aatcgctcac aggatccagc acacctactt taaagcccat ttcttttgca
     2401 ctaatcgcca tcatacgacc taattgaccg ccaccgacga tcccgatcgt tgaaccaggc
     2461 aacaaaggtt taaccaagtt gatcactact ttctataaca gttttagcca acattgaacg
     2521 tctttcagcc aatttagcct ccaattctaa atcatacatg gaaagcatct gtatcgcaag
     2581 tagtccagca ttaattgctc ccgcttttcc gattgctgta gtagctacag gtactcctcc
     2641 aggcatttgg acaatcgaaa gaagcgaatc caaaccattt aatgttcgtg attggaccgg
     2701 tacaccgata accggcaaag ttgtttttga tgcgaccata cccggcagat gcgcggctcc
     2761 ccctgctcct gcaataatga ttttgattcc tttttttcga gcgttttgtg catattcaaa
     2821 catcaaatct ggggttcgat gagcagaaac gacctttttt tcataagaca caccgaattc
     2881 atccaatatt tgacatgctt ctttcattgt ttcccaatct gaagtactcc ccataatcac
     2941 agagattttt gctgtcattg tttttcctcc cagtttttct acattcatat tttaacaatc
     3001 actttttttg atgtcaaaga aatttcgaat gttaacaaag aaaaacattt tattgttcgt
     3061 gtttattaaa gataaaaaaa aggaatgtga caaaagttag gtcacattcc tttttatgga
     3121 taaacggtgt tctcctgcca actcctcggt cacaaggaac aatattcaac aaacatgaga
     3181 agcttttttt tgaaattgtt ccttgtgact cggagatgaa cggcaggctc acgacctctt
     3241 ccttcaacgg tgttcaatca gctgaccctc ggtattagct caaacaaacg gcaaaatacg
     3301 aggaactatt tttccgtttg tttctctaac aatcaggtca aaacgctgat ttcacaacct
     3361 ttcttttgct ctattacttc ttgaaggctt cttccaatat ttcttcatct tttttcaatc
     3421 cattaaagat caaattcaat atgatagccg taatgctact cattacgatg ccatttccag
     3481 tgaacatttg caatgtttgc ggcatttgac tgaataatgt cggcgtaaca ttgaaaccca
     3541 gcccaaatcc aacagaaaca gcaacaatca atagattttt atcattgctg aaatcaactt
     3601 ccaacagcat acggattcct tggactgcca ccatcccgaa catgactaac attcctccac
     3661 caagtaccgg ctcagggatg atttgtgcta acgcaccgat tttagggaat aatcctaaaa
     3721 caatcaagaa gaatgcagag aaatagatgg gttttcttgt tttgattccc gaaagttgaa
     3781 ccaatccgac attttgagaa aagccagtat aagggaatgt attgaaaata ccgcctaaga
     3841 taacggcaag tccttctgca cgatatccct ttttcaattc ttcttctcca acatttttcc
     3901 cagtaatgtc accaagtgca aaatatacac ctgttgattc taccatgctg accatggaaa
     3961 tgatgatcat tagtaaaatc gaccatacgt cgaaagttgg tttaccaaaa taaaagggtt
     4021 gtggaacatg gaacaacggc gcatctccaa tcgcgctcag atcaatcatt cccatgaaag
     4081 cagctaagat gcttcctccg actaaaccaa tcaataccgc gattgaacga atgaaccctt
     4141 ttgccagcat ttgcacgcct acaattagga gaatcgtcac aaatgcaagt agcagatttg
     4201 ttttgtcacc aaaatcagct gctgttgcat tacctccacc cattttctcg attgctacag
     4261 gaatcaaagt caaaccaata acggtgataa ccgttcctgt tactatagga gggaatagtt
     4321 ttttaatttt agaaaagaca ccagaaacta acacaacaaa gatcccagaa gcgataatag
     4381 atccgtagat agctcctact ccttcattgg ttccgatcaa gatcagtgga gctactgctt
     4441 ggatcgcaca tcctaaaacg acaggcaagc caattccgaa aaatcggtta actgtcaatt
     4501 gcaataatgt cgctaatcca cacataaaga tatcaattga aatcaaataa gtcatctgtt
     4561 cttgattaaa gcctagtcct gtaccgatca aaagaggcac cgctactgct cctgcataca
     4621 tcgctagcag atgctgcagc cctaaaaccg cagcttttcc attttgtgtt tctttttcca
     4681 cgattatgcg tcctcctcaa gaaactcaac ttgtccattc gtcaaggaag caatacgcgc
     4741 aagagaaacg actctcaatc ccatttcttc aagtaactga cgtccatctt ggaaagactt
     4801 ttcgatgacg atcccgattc cttctacttg cgctccagct tgctggcata attcgatcaa
     4861 tccttttgcc gcttgtccgt ttgccaagaa atcgtcgata atcaatactt tgtcttctga
     4921 agataaaaat ttacgagaaa tagaaatcgt acttgtcact tgtttcgtaa aagaataaac
     4981 agaagctgtc aataattctt catccatcgt caaacttttt gcttttctag cgaagatcat
     5041 tggaacatct aattctcttg cagcaaataa agctggtgcg atcccagaag cttcaatggt
     5101 cacaactttc gtgattcctt gttctttaaa tacttctgca aaccgttttc ccatttgttc
     5161 catcaataca gggtctactt ggtgagtgac gaagctgtcg acttttaata cgccctcgcc
     5221 taatatacgt ccgtcttttt gaatacgttc aactaattct ttcactttat ttgctccttt
     5281 atcatttatt aataccgaaa taaccgcttt tgtgcaaaaa cctactatgt ttctgttaaa
     5341 gaaaaacacc ctccacttta tcataagaaa agaagggtga aatagccaat cttttactta
     5401 tagtccagca tttacggtac tggctagaaa ctgtcatcca tattatgacg atatataagt
     5461 aggtatgtaa aactaataat agtgtaaatt cattttcttg caactgtttt gcacgtgaag
     5521 gcagtttgcc atatttgaat aaaaaatgtt gtttcaaata ttgaaaacag tctaacacta
     5581 ctttctgaaa ctgacaacgt ggtttactta tttttttgta aaatctaatg atttttaatg
     5641 cattattcct ttttgttcgg gtttaaatag ttatttttat tttctcctat ctaaaaaagc
     5701 agcccacata aaaagaaggc atgacaaaaa gcatgtcata gccccttttt ccgaataaat
     5761 agtgttcaaa agacagccta tcggcaataa ggcaagattt tataaactac gggaagtaat
     5821 ttccgtaaat ctattcttat tcttttaagc tgacaagctc gtcaatcact actgcttttc
     5881 ctgaagcaat agaatgattt gctgcgattc ctgtcaaaat cgacaaaata ccatctacgt
     5941 gagttgctgc ccgattccac ggatcttctt ctttttcttc accaaataga tcatctaata
     6001 aacgcggatc tcctccgcca tgtccgcccg cttcttgttc gacgattacc tcatatggct
     6061 caccgaacat cggatagaca tggatggact gaagaacggc tgctccttca tcctttttat
     6121 ctccacctgc attgatgtaa gacttttcga tcactttcat ttcgatccgt cctttgctgc
     6181 cattgaaaga aacattgaat ccttcccacg gcatatacgc gtttagcgaa tacgtcaaaa
     6241 tcgcaccagt ttcatatttg accgttacgc ctagtgtatc ttcaatagag atatcatcgg
     6301 aaaatacact gcggtcacga atatatccac tgtctttttc tgcttctaaa tacatttctc
     6361 gcaattcctc attggctgac aggtctatag caaatggatc agctttagct agttcgcttc
     6421 catagcatcg actgtaaaat atttctactc cgcgtttctc cgcattatgc atcccataaa
     6481 agtttaatcc accgaatgca aatacctttt ggggacggct gttcaaccaa aaattcacta
     6541 gatcaaaatg atgggtagat ttatgaacaa gaagcccgcc gctattgtgc ttatttcgat
     6601 gccatcttct gaaataatct gccccgtgtt gcgtatttaa cagccattcg aagtggatag
     6661 aaaacacatc accaatcaca tcactttcta tcagttcctt tatcttcgtg tgatacggtg
     6721 cataacgata attaaaagtg actcgcaggc ttcttcccgt ctgtttttgg accgttatta
     6781 tttcttgagc tttttcctca tcgatagtca gaggtttttc gcaaataaca tcatatcctt
     6841 ttttcattgc acgaatgata taatcatgat gcgttcgatc tactgacgta ataatgactt
     6901 cagccggccg tacgtcatcc agtagttgct caaattgtga tggcatacat gtctttactt
     6961 ttttctgtcc atacttgttc acaagtatcc gatttgcata atccatcctt gtttgagata
     7021 catcacaaaa accgactagt tcacaagttt gatgatacgt agaggcaatt gcctcataaa
     7081 aaaaacgcgc tctcccacca agtccgactt gtacatattt tttcttcatt taaagtcatt
     7141 ctccttttct tttaagcaaa atactgtgac aaatggcagc cctggataca gtaatgcagt
     7201 gagtaaaaaa tcatgctgta tacaccagaa cagtccatac aaaaggcata aataccacag
     7261 aaaccccttg acgttttttg acaacagcca gatattcctt ttgatcaaat aaacaagact
     7321 atctgcttta tcttctttgc agatatcaat tagcgtacta aaaaaactat gggatacaaa
     7381 aagcaacaga cacaatagaa aaggaatcag ataccaagct ccaatctttc ccactaataa
     7441 ataccaatca actgcaaaaa taatgacaaa cagctgaaac aacgtaatga tgaaacacat
     7501 ccgtttgatt tttcctttcc tccaacatac ctttttttct gccaagtaat ggacagatag
     7561 ataataagaa ggatacatca tcaaaaaata aatcagatag acaacacgag cagacagcac
     7621 taatcctaaa gtgaacaaac atcctagaac taaagcgaaa agagcattac tgataagact
     7681 ggttaaaaaa atccgacaga catagtagtt gagcttgtaa agaaacatct cttctattgg
     7741 ctttttcata ttcagccctt cagaccgctt gtactgatcc cctggacaag atatttattg
     7801 aagaataaga aaatcagaaa aaccggtagc agcgacaacg tagacatcgc aaacatactc
     7861 gcgtaagtcg tactagatcc agcgtctgca aataatttga tggcaatcga taccgtgtaa
     7921 gatctcggct tgtttaaata aatcaatggt cccatgaagt cgtcccattt ccaataaaac
     7981 tggatgatga tcgtcgtgat gatcgaaggt ttcagcaaag gaaaaataat tttggtaaag
     8041 acactatact tgctgcatcc gtcaataatc gctgattcat ctaactcttt ggggatgctg
     8101 accatgaatt gcatgatctg gtaaatgaaa aatgcttgtc caaaaaaatg tggtaaaatc
     8161 agcggcacat acgtcccaac caaattcagc cgattgtaaa taataaactg cggaatcaaa
     8221 atcacttgtg caggaatcat catcgttact aacatgacga cgaaccagaa tctttttcct
     8281 ttgaaatcga ttctcgataa tgcgtaagca atacaagtcg agctcagaac tgtccctatc
     8341 gtcgaaactg cagagatgac aaacgaattc ctaaagaaag taaaaaatga aattccagca
     8401 aaccccgccc atccttttgc gtaattttcc cattgaaaag tctctggaat caaattcagc
     8461 gaaccactca taatctcttg atccgtttta aaagaacctg cgatcatcca taagaccgga
     8521 taaatcatga ctagacctaa tagcaaaata aaaccgtatt gaaaccattt cgttactgta
     8581 ttattcattt cttcctcctc tcattcacac aacgacccat ttttttgatg atttgaaaat
     8641 cagaaaggtg acagctccca taatcaacag catgatccac gaaatagcac tagcataccc
     8701 catagaatta aaagaaaaag cttggttata cacataaaga gcataaaagt tcgtggcatc
     8761 attcggacca ccttgggtaa tgataaatgc ctgcgtaaaa gtcataaacg ccgcgattgt
     8821 ctgcataacc aagttataca aaatgatcgg cgaaagaaga ggtaacgtga tcttgtaaaa
     8881 cgtctgccat ttgttcgctc catcgatctc tgccgcttca taatacgagc ctgggatctc
     8941 tttgatacct gcagcaaaga tgatcattgc cgaaccgaat tgccaagcag acatcaaaac
     9001 aagagggatc atgaccagtt tttgatcgct aaaccaggaa agcgcaggtg cgcctaatcg
     9061 ttccaggaaa atattcacta acccgttttt agaaaacaac tcccgccaaa ctagagcgat
     9121 agaaatactg ccaccgatca aactaggcac ataatacaaa gaacgaaaga aagtcgttcc
     9181 tttgatattc ctcgttaaaa gataagcaat caatagacca aagatcagtt tcacaggtac
     9241 agatgcaaat acatagagaa aagtgactcg gatggagttc aaaaaacggg catcactcag
     9301 catctttata taattatcca agccgatcca tgagactgga tttcccaaac gataatcggt
     9361 gaacgaataa taaagtgaca agatcatcgg tatcactgtt atcaatagaa atcctaggat
     9421 gaatggagaa gcaaatacgt aaccgaccac attgtcttga tccaagaacc gttttagttt
     9481 ttcttttttc atgccttcct cctgctgcta tcctagaatg tttgacgcaa actcataaag
     9541 actctctgct gcttcttctg ctgttacttc ttcataaatg actttttcaa ctaaaagatt
     9601 gtattggtct tgaatttccg gattcaaagc actatcaaag acattcactt cgccgttgtc
     9661 catatcggaa atcatttcta aataatcaaa tgtcgcttgc aaagttgcat catctgcacg
     9721 agcgagctgt tctcgaatat tcgacatgat tggtacacca cgttctcctt ttaaaatttc
     9781 attggcttct tcactgtttt gaaagaaatc aataaactta gcagcttctt ctttatgggc
     9841 tgagcttttt gccatactca gcatttgcga agaacgtaaa gggataccag attctccatc
     9901 tgcttttctg cgaggtggat taatcaactg aatctccata tcttcaggag cccctgacaa
     9961 tatttctgtc aactggttac ttgatagaaa caccattgcc gcttctctat ccctaaccgc
    10021 ctgaaaagac agatccgtcg tacttgcagc tccaccacct ggttctggat gggccccttt
    10081 gtctaccaga tttttacgca gctggaaaaa atctgttaga tagtctggat tttcaaatcc
    10141 caagctgtta gatagatctt cccgattata aaaattcaat cctttctcaa cttgtggtat
    10201 ttgaataata cagccagcta taaaatcttc gaatcttgaa atagcataga ttcctaattc
    10261 ttcagtgatt tgatagctga tttgttcaaa ttcatcccat gtccaatttg aagctggttc
    10321 agggatattt aattgttcaa atatattttt attgtaagca atcgcaaacg aattcacccc
    10381 gttagacagt ccaactaatt gcccgtcgta agtcgtcgtt gctaaatagg attcatctgc
    10441 attagaaata tcaattatcc cttgctccac atattcatct aatggttcaa tctgattgat
    10501 gtattgagga aattgattgt tcagttggaa aatgtcccag atatcatctg atgctgctag
    10561 tgtacctaat tttgtgtaat atccttcata atcataaaat tcataatcga tcgtcacatg
    10621 gggatttttt tcttcataaa gctcgattac ttttactgtt gcatcgtgtc ggacttgcga
    10681 accccaccaa gccatgttca gcgtgatttt tttgtcatct tcttgcccac aagccgtcaa
    10741 gaagaataac ataacactta aagctaagat gattctcatt cttttcttca ttctctgcac
    10801 ctctacttcc attgttattt ttcaatatac tgtattttta tacagaacat atttattaaa
    10861 cgatcttttt caaggggaat aaaaaccaag acttgcctta tcgttctgcc taggatgaac
    10921 aactcaatac tttcaagaaa aattagatag tgctgattct tctttcatcg ctaaaaacac
    10981 ttcttgcttc aatgatggga ggataattac gcaaagaaaa aaggatatca gacaactgcc
    11041 caatatcctt gacatagcta aataagcatg cttcttcgtg tgcgctcttc cctaaaaaac
    11101 agaaaaataa ctagtaattc tgtatgaaat taggtacaat atacacgtcg atgcacaagc
    11161 attgactatg gcaacttgat tggtcagttc ccgctgactt ttcaagcccc gacaatttag
    11221 ctgctactaa attgtcgggt gcctttttta gtttttattc gtttttcatt ctatagaaag
    11281 cgcttcaata aatcaagaca tttttttgtt agaatgctaa taataacaaa aggttttaac
    11341 tcttcttatg tacattacag gtaaaaaaac aaaaaaattt ctcttttttt tgaattctga
    11401 acaaatagta ctggagtaca aaagacgagg aagagccact atctgctctt cctcgtcttt
    11461 tgattctcat ctgttattca tgattctttt tctcagtttc ccgatatttt tccattttac
    11521 gactaaaaag ctctccgcta cttggtggcg tatatgtgat ggcatttgct cctgcttgaa
    11581 tggtctcagc aatactttct tccgttggac cacctgtcgc aatgatagga agttcaggat
    11641 attttttacg aaaatggcgt actgcttctg cagtttcttt acctgcactg atattgataa
    11701 tcgaaatacc agctgctaac ttttcatcta tttctgtata tttcgacgtt accgtactga
    11761 taactggaat atcaactact cgacaaactt cttttactgt ctctaaaggc gtaggaccgt
    11821 tcaaaacgac ccctaacgaa ccttgcgctt ctgcgaacat gctcatgtag gaagaacgag
    11881 caccttgtgt caaaccacca cctacaccgg agaaaactgg aatgtcagct gcctcaataa
    11941 tgcttttagt aatcgctgga tgaggtgtaa aaggatacac agcaattact gcattcgcat
    12001 ttgtattacg tatgatcgca atatccgtag taaagatgat cgaacggatc aattttccaa
    12061 atatcttgat cccgcttgct tctaaaatga tttccggtac tcttacgata tcttttctca
    12121 attccgtcat gatttctggc gtaaattgtt ctgccatcag ccacttctcc tttgctatct
    12181 ccggtcatac tgattcaatt gatacgtctc gatgacctgg atgatttttt gcgcataacc
    12241 ggggtctgtc gcataacctg catcttgcaa tgcttgtgcg gcttcctgat agttggtggc
    12301 cgtaatcact ttttcataca gcgcaggatt ccaatctact ccttggacga acagttgtgt
    12361 atggtcatcc atggactgtt cccatgaatc atacactttg aagtctcctt ggatagttat
    12421 ccattcttcg ttaacaaatt cctttgtttc caggcttact ttcttctgat ccccataagc
    12481 cttgatcccg aaaagattat tgtatttgct agccaaggta ctttttcccc aatttgattc
    12541 taatatagct tgtcctaaaa tgatactagg aagcactcca taaccatctt gtagttcttg
    12601 ggcatgcgga acaagccgtt caataaattg ctgatgggat tgcgcttcat tttcttgctg
    12661 attatcagta aaaggagaag acagattctt caaggaaaaa acaaaggcta agagtatcac
    12721 tgctacaccg gcaaccactg ctggccaacg gatattcttg cgcttttgct ttttccactt
    12781 ttttttcgcc atgatcaatt cctacttttc atttaattca ttcagatatt cttctagcgt
    12841 caattggtgt ttggtgatat actcagcgtt ttctttaccg acataacgta agtgccaagg
    12901 ttcatatgtg atcttagtta tatcttcgtg tccttttgga taacgaacga taaatccata
    12961 ctgcgcagca ttatctgcga tccatttagc tccaggctga tctccgtaac ttgcatccaa
    13021 tacagtagaa ggataactgt tgtaccagtt ttgatcgacg acatcgactg ccagacctgt
    13081 gtggtgttcg ctgtatcctg gctccgtaat cgtttcttta gtaatctttg tcgcctcctc
    13141 ttctgataca ccttgacgac tcatcacatc ttggacattt tgttcgaaaa cttgttgttg
    13201 ggatgataca gaccggtaag ctgagaccat cactaatgga aatccagctg cttcagcagc
    13261 cttggcaaat gcttcatatt ctggagcaat gcgtttgtcg atcaagtatc cgttacttaa
    13321 tgatgccaat tgagtagact catcaatttc tttttctaat ttgtgatctg gaccaaccaa
    13381 aactagcttc caatcgtctg ttttaacatc tggcaattgt ttggacgtac gatcttttgt
    13441 ttctttgtcc tttaccttgc tgctggttgt ttgactgttc ttttctttac tagcagttgt
    13501 tttggtgctt gttcgttcga ctgcttgatt gccggcttga acatgcgaat catcatcgct
    13561 aaaaataaca tagcccattg ctgcgatcat aatcacaact gctccaaatc ctaaaaattt
    13621 attcacttga aaaatccttt cgttgttctt cacgttcccc attcgcctca tgaacgtaag
    13681 tttcttccag attttcattc acccaatcga gaagttcgat tttttctcct tcgatctgtt
    13741 tttgaacatc tgctggcaga cgaaaattca aaatatcatc atagccccat tcatcatatt
    13801 gaatacttac tttcaagtca aggtatctac cagcctgctg attatttttc aaagggatca
    13861 gctcgacttt tgctgctttg ctgctaagcc atttttgcgc gatgcgtgtt ttgatttgct
    13921 tgtactctgc aacggcaacg tttcgttttt cgggaaaaat aatcgtttga cgcaaaatct
    13981 tttgcgttag catccattca taatcagtga aaagattttt tactttctgc atcgcttcag
    14041 atggcagatt tttctcgcca tctttccatt tttgccattg ttctggactg atcagcaagt
    14101 attcatctaa aaaatttttt tcagtttcaa actgttctaa taatacactc ataataagat
    14161 caatatatac ttgatgcacg tttatcccct ccttttaact attctactta agttttgatc
    14221 aaaaagcacc tcttaaatcg ctgctttcat tttttttaat gtttcaagtc ctctcctctt
    14281 tattcttcgt gatttttgtt ataataatag acagacaaga aactcaatcc aactatgaag
    14341 gagcgttttc agatgaaaaa atatcaatgg ggaatcgttg gtcttggtac catcgcacat
    14401 gagtttgccg aaagcttcaa tcaagagacg agcgaactaa cagcagttgc ttccagaact
    14461 tcagaaaaag cggaaaactt cgctcaccgt tacaatattc caaaagctta cggaagctat
    14521 caagaaatgt tagacgatgc tgaaatcgac atcgtgtaca ttgctgtacc aaatagacag
    14581 catatcgacc atatcttggc agcattggaa gcaggcaaac acgttctatg tgaaaaagcc
    14641 atcacgatga ataaaaaaga actggctgac gctatgcgct tagcagaaga aaagaatttg
    14701 atcttagcag aagcgatgac aatcttcaac atgccacttt atcagcagtt acgctcaata
    14761 atggatacag gaaaactggg ggctttaaaa atgatccaag cacctttcgg cagttacaaa
    14821 gaaccagacc ctaaaaaccg gttcttcaat ccagaattag ctggaggagc tctgttggat
    14881 atcggtacgt atgctgtatc tttcgctcgt ttctttttga gctctcaacc tgaagtagtt
    14941 gcctctacaa tggttccttt tgaaacaggc gtggacgaac aatccgtcac tatcttgcgt
    15001 aacaaagaaa atgaacttgc tgctgtcagc ttgacttttc aggcaaaaat gccaaaagtc
    15061 ggtatcgttg cctttgagaa cggttatgtg acgattgctg attacccgcg tgctgatcgt
    15121 gcggagatct tatttacaga tggtacaaag gaattcatcg aaagcgggaa tacatctgaa
    15181 gcaatgaatt acgaaataaa gaatatggta cacatgattg aaggtgactt gcccaatcgc
    15241 tctctcttct tgacaaaaga tgtcatcgaa atcttggatc aaatgcaagt tctatggaaa
    15301 aatatgtaat tttaaaaggt tgtgaaaaag ctgttctgca tcaagtaata agaacagcca
    15361 aacaaaaata gctcctccta tttttcgttt ggctggactt attaccgcag aggaaagctt
    15421 ttgaacaccg tttaagaaag aggttgtgag cctaccgttc agctccgagt cacaaggagc
    15481 aatttcaaaa aacagcttct catgtttgtt gaatattgtg acttgtgacc gaggagttga
    15541 caggagaaca ccgtttaaag aagaggttgt gaaatcagct ttttgacctg aatattagct
    15601 gaattcacaa cctctatttt tggatttttt gttgggagaa ttctgccttg atttcaataa
    15661 aacttttaaa agactgcaaa aactggaaca attctgctcg atcctcgaat tcagggcgac
    15721 tagtcggcag aggcatttgc cgatacaata cataaacttc ttcaatacgt gttaaaattt
    15781 gtctgccatc atttgactct gaaaatgttt ctgcagtata aatcaataaa ccatagatat
    15841 gttttccata ataagctggt tgctggattc tctctagatt ttccagcatg tctttaatca
    15901 cattggcctg tgctcttctc atagagaagt aagtttcgta gtatagcggc tgtctgagcc
    15961 agtagttttc ttgatgttct ctggcatctt cttggctttc gcgaatgaat gtcagtaaat
    16021 gttcgcattt gatctgcaca tcttctttat tttctgataa aagaaattct gccatttgtc
    16081 tcaaaatatt tcgaaattct ttttcgatct gtatttggtt ttccttcagc tgcttcgtac
    16141 gatctggcat gtaaacattt gcaagcaaag caaatactaa tccaatcgcc attaatcccg
    16201 cttcattgcc gatcagctgc caagacatgt tttcctccac taaataatga gtgaccaaaa
    16261 ccgaatttac tactatccct tcggttaatt tgaagcgaga ggcaagcggg atgaacagga
    16321 ataaaaaaag actgaagcca aaaatagtat acccaacagc aaaaaaactg aacagtgctg
    16381 ttgccgtagc taatataaag gctgcgattc gattcatgcc cgtacgcaat gtagagcgct
    16441 tggtatttcc tacacttaat aatgcaatga tccctgcagc tggccaatac aacagcgata
    16501 agctacccgc tgtgaccatc gaaaagaaga cactgatcac tgttttgact gttcgcattc
    16561 ctatacgcat ttacttcccc cttttttatt ttaattatac ttgaataatg aaaaactagc
    16621 tatcagacaa aagtttaggt atgatagtaa aaagaccaaa aaggagaatc tctatgcaat
    16681 ttggccgttt tcgactaggt atgcgtacga tcaaaagtgc cttagctgtt tttttatgta
    16741 tcttgttttt tcatttgact caacgaggaa tcccgatgat tgctgcatta tctgctgttt
    16801 tttctttgcg tcaggacttg actacaaccg tttcttttgg aaagtctagg atcatcggca
    16861 atacattagg tggtacatta gctatcattt atttttttgt caaaagctat tttcacaatg
    16921 attttttggt agaattgatc ttgcttccta ttctagttat tattgtgatc gtcgtgtcag
    16981 atggtattaa caataattcc ggtatcattt ccgcaatagc tacgttgttg ctgattgcgt
    17041 tgagcatccc tcaaggcgaa tccgctttgt atgctatcca acgtgtacta gatactttta
    17101 tagggacatt cattgcaatc ggtatcaatt tctttcttcg tcctcctgaa acagaaaaga
    17161 aggaagaact atcagaagat ttggtcgaac tccaaaagaa agaacgattg ctaagaacaa
    17221 atttacaaga gatagaagct aagatccaaa aacaaaagaa aaatagcgag gactaatttt
    17281 cttttgcaat aaaataaaaa aatggagaga agaactttaa aaagtcttcc tccatttttg
    17341 attgaacggt gaagtgtact actttattct tccttcatcg agttttgttc tgcctctttt
    17401 ggcgcttcat acatatttac ttggctagta gtcccacgtg tcgacaaatt ctctacgatt
    17461 tccttcaagt caagtcctgt tgtcgctttt agtgtttctt gagtagaaga aagcaaattt
    17521 gtggcataat ttgtcactcg gttcgctcca ctggtttctc ctgaactgct tcctgtatcg
    17581 acaacagaga ttttttcgat atttcctagc ggctgtgctg cttcgcgcat caattgcggt
    17641 agcatatcga taaccatact caagacagct gcttcgccat attccttgaa ggcattggcg
    17701 atcttttgtt ttgcttcggc ttctgctttt ccttttgcta gtgctgcttc ggcttctgct
    17761 tgtccagcta aacgtgtctt attggcttcc gcttctgcca gtgcttccac acggaatcgt
    17821 tctgcttcgg cttcagtcac ttctttgact ttttgagctt gtgcttcttg ttctttagcg
    17881 taccgatcgg catctgcttt tttcttcact tccgaatcat attgcttttc acgacgtgtg
    17941 atttcttttt cttccagttc gatttgtttt tgacgttcta cgattttaat ctgcatttcc
    18001 tgttcgatta cttgttgttg tgctcgggca ctttctaaat tgtaggcttg gtctgctttt
    18061 gctttagcaa catcttgttc ttgtttgtag gttgctaatt tcagttcttt ttcttttaat
    18121 gattctgcga tctctgtttg tcgctgtaat tcagctgctt gtgattcttt ttctgcttga
    18181 gcttttttga tacgcgtttc ttttagagct tccgcttctg ctatatctgc atctcttttg
    18241 acttgggcga ttcttggttt ccctaaagag tcaagataac cgtttttgtc tctgacctct
    18301 ttgatagtga aagaaacgat gatcaacccc atttttgcaa ggtctacaga agcgacttct
    18361 tgtacgcttt gactgaactt atcgcgattt tgatagattt cttctacagt catagaacca
    18421 agaatcgagc gcaagtgtcc ttctaatact tctcttgctt cattttccag ttcttctctt
    18481 gtcttgccta aaaactgttc tgctgctgta gcaatctctt ctacagatga accgattttg
    18541 atgatcgaag taccatcaca catcaccggt acgccttgtt ccgtatacac ttcaggggtg
    18601 gagacgtcca atttactcga taatagactg atccgattcg aacgttgaaa aactggcaag
    18661 acgaatgcgc cacctccacg cacaattttg attttgttat tgctttcatc aatgtgcaca
    18721 tttttgcttc ctaaataact tccactgatg atcaacgctt catccggttt tgctgtctgg
    18781 tacttcgcta caaagacgat cagcaacatc agtaaaatca aagctgcgat tgcgatcgcg
    18841 ataaaaacaa ctggtatttc catttaacac acgctccttt aagcttttat catgctttca
    18901 taaggcatga cataacagat tctttccttg atctcaatga tcaatacttt cgttccccgc
    18961 tcagctgtct gttcttgggg gctgtaaaaa caagctggac gactaatgga accggtaaca
    19021 cttttcaact gaatttctcc catacctcgt acagggatcg gtgtgatcac aacagctact
    19081 tgtccttcca gagttttttc agaagacgac atcgttgatt cggcatgttt catcggcatc
    19141 aatatataga aattaagcaa aaataccaac actatcgata tgcctccact agcaataaat
    19201 aaaaataaag atgattgatc tgttaaacgc tcacctagaa aaccaaataa gcttgtgaaa
    19261 gttaaccaag ggataataag catcggatca agcgggccat caaaatcgaa tacatctcca
    19321 aaaataatca ataggactgc caatcctgca caaatcagca atgtatacca ataaatcgtc
    19381 tcaagtgcga ttccccctat catatacacg cctccttcca tttctacgtc tatcatacca
    19441 atgtttgaaa attctgtata gcttctttta ccgactcagt ggtgtttttt ataggaaggt
    19501 tgtgtttatc gttgacaatt tgttaaattt ttgttttcat gttaaaattt tcacaataaa
    19561 ttctttcgag gtagcctatg tttccttttt ttaagaaaaa accattaaag ctccagaaca
    19621 cgatcatcct tgtcgtttgt ttctccacat tcgtttcttt gattgtttct gccatattga
    19681 tcaggaattt tattctttcg atgaaccgat ctagtgtgtc agataaaatc tctagcattg
    19741 ctaaaatcgt tgccaatgat aaactcatcc aaaaaaagct gcagactggt gacccgacac
    19801 atgcgattca aggaataaca aatgaaatca tgattgcatc gaatttagat tttatcgttg
    19861 tcatggaccc tgaaggcatt cgatattctc acgcagaccc aagttacatc ggtcttcctt
    19921 tctcaagtca tgaagatgtg aaaaaagtga ttggaggaca agaatttttc tctgaaaaag
    19981 aaggaatgtt agggttcggc ttccgctatt tcgtccctat ttattatgat caagagatta
    20041 ttggtgcagt ttgtgttggc ttaacaaaca acacgattga caatcaagtc aaagatggac
    20101 aaaaaatgat tgttttttca ttgattattg gacttagcgt aggcatcata ggcgccgttt
    20161 ttcttgccca aaaaatgaaa aaagtactgc ttggacttga accacaagaa atagcagaac
    20221 agttaagtga aaaaacgatt atcgaaaatg aagtagatga aggaatctta gccatctctt
    20281 ctgataaaac tatcctcttg atcaatacac ctgcaaaaaa cattttctac caaacagagc
    20341 agctattgga tttcgaagta ggaaaaaaat cagtgataat ctatatgacg ttttgtttaa
    20401 agagacgatt gagcatcaaa cgaccgttcg gactcaatcc atgtatttaa atgggataga
    20461 aactatttct agcgttacac ctctttttag caatgaccgt tttttaggag cagtcgcaac
    20521 tttacgaaat cgatcagaag taatgaaatt aatggaggag cttgggggga cgaaacaata
    20581 tataaactcc atgagagctc aaacccatga attcatgaat aaacttcatg tcatttctgg
    20641 tttgattgat ttgaagaagt atgaagaagt cacccatttt atccagcagc taaatcaaaa
    20701 ttataaaaaa gagattggcc aagtctcaag tctgatcaaa gtacctgcaa ttgccggttt
    20761 tttattaggc aaaataaacg aagccaaaga acaatctgtt gaaatgctgt tagaaagcga
    20821 ttcttttatc cctaaaatgg agatggatga ctccatccat tcacttttgc aagtgttagg
    20881 gaacttactt gataatgcaa aagaagccgt gctacaaaaa catgaaagaa acggacaggt
    20941 ccggattcat ttaaactatg acacagaagc tgaagtattc atcgtgaccg tctgcgatga
    21001 cggtgtcggg atatcagaag aaatctcaga aaaaatattt acgctaggtt tttcgacgaa
    21061 gggaatcaat cgtgggtttg gattgaatct catccattca atcgtcaaac gccacggtgg
    21121 atttttagaa ttttccaaac aaacaaatgg cggtacaatc gtctatctag aattaccaaa
    21181 aaaggaagat caaaatgatg acaaatgtcc tgataatcga agatgatcca atggttgcag
    21241 aattgaatcg tacttttgtg gaaagcatcc ctaatatgaa cgtaatcgaa agtttcagaa
    21301 atgtagaaga aggaaaaaaa tatttcagac aaaaaaacgc agatctgctg ctgcttgatg
    21361 tatacatgcc tggcgaaagt ggtatccatt ttttatcttg gcttagacag caaggaaatg
    21421 atatcccagt gattttgatc actgctgcag atgatatcga aaccataaag aatgtgatcc
    21481 gctacggtgc cttagattat ttgatcaaac cgttcacttt gcaacgtttt cagctggcga
    21541 tcgaaaagta tcttgctttc tctcagacaa tccaaacgac tgaaaaagcc gatcagaaat
    21601 tgatcgatca agtattcaac cacaccactc ccattcacga aataccatca ttcatcgatt
    21661 tgcctaaagg tctgtcaaaa ctgactttaa aaaaaataag tgcatttatt ctaaaagaag
    21721 atactatttt ttcaacaaaa gaactggctc aaaaaagctc actgtcacgt atatccgtca
    21781 agaaatattt gaatttttta gtagaacaag atatattgaa cgaaagaatc aaatatttgg
    21841 aaatcggccg gccgctaacc tgttatgaat tgacaaagaa tgggcggaaa aagctcacag
    21901 attactgttg agtcatcgaa ttatcccata caaaatagtc caagtataca gaagtaacaa
    21961 ctgtttactt ggactatttt taaacatttt attgacgaat atccatgttt tcttatctta
    22021 attgacgggc tttaggaaga gatacctcat ttttcgtttt tccagtttct attatttcac
    22081 gaaagttctc gaaacttgtc gaaatcatat ttgataatgc ctcatctgta tttgaaccta
    22141 catgcggggt tacaagaact ctagggtaca tttctactaa ttgctggata gcagggtctg
    22201 gaatcgtctc ccatggcttg aaggatcgga aaaaaacctc tgtttcattt gcaaaaacat
    22261 cggttgcaaa accagctaaa tgattgcttt tcaaagctct taaaatagct tcattatctt
    22321 gaagctctcc acgggctgta ttaatcaaga tgctaccttt tttcatttga gctaaaaatc
    22381 cttcattgat catttgatcg ttttcacctg ggatatgagg tacatgaata ctgacaatat
    22441 cgcttttggc taataactct tcaagcgagt ggaacgtcaa tactttttta gcagcttctg
    22501 tctgatgaat atcgtagccg atcactttgg ctcctagtcc cttaaacaaa acagcttccg
    22561 tcaaccctat cctgcctgtt cctatgatcc ctactgtgca atttctgatt tcacgactaa
    22621 acatttgctc atccactcga aaatctttga aagatgttcg gatcgtcgta tacgcagtat
    22681 ttctcaaaag catcatggca aaagtcaaag ataactctgc tatcgcattt ggcgaatacg
    22741 atggtacacg ggctactgtc ataccaaaat cagctgccgc ttgtagatcg atatgattga
    22801 ctcccacagt ccgtgtaaaa acataacgaa tcccatattc ctgcattttt gcaagattgg
    22861 tctcatctgc tatacaattt cccctcaaca atacagcatc atggtccttt gctgacacga
    22921 tattgtcatg tgtcaacagt tcttcataaa gactgagatc ataatggtaa atattcaatt
    22981 tttcaaaaaa atctatctcg ttcggacgaa caccgtaaca tgcgattctc atactttctt
    23041 cctccatttc ttatgaaaat aaacctatgc catgaatcat ttgtaataca acgatccata
    23101 caggtgtcag taccacagca aaaatagttg acagtaagga tgcattggag gctaaaagtg
    23161 cctccctatc aaaactgata gcataggctg ctgccactgt tgcagctggt gtagccatca
    23221 tgataacgat tgttgccagt gcttcaaaat tgactggtag aatattcgtt gccgtcaaaa
    23281 ctgctagaat cacgatattc aatgcaggta caaataccac tttgactaga ctgtaatacc
    23341 atgaagtttt ttcagatgca gcttttttga agcttacttc acctaatgtt gcgccgattg
    23401 ctagccacgc caatggtgaa cttaaaccag ctaaataatc catcgactga aatagccacg
    23461 gtgcagtctg gtcaattcgc aaaaacgcta cttggcttat ctctccttct gcacctgtaa
    23521 ctgacacttg tggcagatac ccttggaaca gccaaatgaa aaggccggca aaagtagcga
    23581 taatgattgg attgacgaac atcgctttga tatttttagc ttccatcttc aagccactca
    23641 tttttatgta tccataagaa tacaaaaaga ttcggtagcc aatattgaaa attgaggaat
    23701 acatcacgcc gacaggacca taaatcgcac tgacgatagg cgttccgaaa aaggtcgtag
    23761 aaccaaagat cgtcaggaca cgcaacgttg tttgcttatc ccctttatag cttaagaaaa
    23821 aaggttttga aactagaata agaataatat aaataactaa tccccaaatc aagacattga
    23881 ttccttgcat cagtaattct gtattgatat cttgcatgaa agctcgaaat gccagtgctg
    23941 gcaaagccac tgttaagacg actttcgata gaatttttcc aacagattca gaaaaaatcc
    24001 ccttttttct acaaacaaac cctaataata taataaaaac tgttgaagtg attgcactga
    24061 caatcgacat atctgtcaaa gtcgatcgaa aaatgtcaaa taagttcatc tttctcctcc
    24121 taattatgtt catttattca caataaaaaa agcttttatt ttgctaatac gtattaacat
    24181 ttttaatttt atctttacat tgttacttat tgaacaatag gaaattaaag aaacttttga
    24241 aacttatgta aagtgatt
//
LOCUS       DAZKLM010000037        24022 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3764
            SAMEA115706913-rid22582563.denovo.037, whole genome shotgun
            sequence.
ACCESSION   DAZKLM010000037 DAZKLM010000000
VERSION     DAZKLM010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706913
            Sequence Read Archive: ERR13196647
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24022)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24022)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287823.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287823.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,004
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,849
            CDSs (with protein)               :: 2,849
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 41 of 96
            Pseudo Genes (incomplete)         :: 52 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 15 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24022
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706913-rid22582563.denovo.037"
                     /isolate="VRE3764"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(55..789)
                     /locus_tag="ACG842_001360"
     CDS             complement(55..789)
                     /locus_tag="ACG842_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MIP/aquaporin family protein"
                     /protein_id="HFQ0247913.1"
                     /translation="MDYSLMTRLAAEMIGTALLIVLGNGAVANVDLKGTKGYGSDWML
                     IAVGYGFGVMMPAMIFGGISGNHINPAFTIALASNGLFPWAEVVPYIIAQLIGAAIGQ
                     LIVVACYKPYYDQTEDVNHVLGTFSTINSVGSKFNGFVNEFFGSFVLFFGALGITKAP
                     FFQDNLGTAHLALGFLVWTLVASLGGPTGPGLNPARDFAPRLVHALLPLKHKGSSQWG
                     YAWVPVVAPICAGVVAVFLYKLLFMA"
     gene            1116..1526
                     /locus_tag="ACG842_001361"
     CDS             1116..1526
                     /locus_tag="ACG842_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HFQ0247914.1"
                     /translation="MKFFQEFFIIIFFSVLGEGIRIFAHLPIPGSILGILFLFLAFEM
                     HLVDPEKIGATGDFLLNNLAILFVPAGVGLLEYFDDIASIWPVLLGAVVVCSIVTMVA
                     AGKTAEGVEALLGYVRKKKAERVSVNEMEENQID"
     gene            1519..2220
                     /locus_tag="ACG842_001362"
     CDS             1519..2220
                     /locus_tag="ACG842_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HFQ0247915.1"
                     /translation="MTEFFVNPLFGLSLTLGVYVLSDKFLRKYKLPVLNPLVLSIVVI
                     ILFLHFSGISYKDYNIGGNFLTMLITPATVALAIPLYKNFRLLKENFYPVMAAILAGI
                     VANCLVSVGFGYFFSLHKEMVISLLPKSVTTAISVDLSHTMGGVNAVTLAIVVSTGIF
                     GSLIATHIFRLFKIESPVARGVALGSTSHAIGTAKAIEIGEIEGIISGLAICVNGILT
                     VLLLPLFFQPFAGLF"
     gene            2220..3806
                     /locus_tag="ACG842_001363"
     CDS             2220..3806
                     /locus_tag="ACG842_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0247916.1"
                     /translation="MRKPLFYATITKTFEHKKGGKDVYLSSEYLKQFDKDLYYKILLL
                     ESFEDQAWHTAAQLAQVVQLDARSVSKYLNELSKNYQQFSGKTHPLFTKNHRSGYNFY
                     DTLDSIEHERFLIYLVQSTLKFQLLHDIFFEEFHTMYQFAQKHYISESTAHRKINEWK
                     QQLQTYGIRLQRGTYIAQGEEEIIRLYLHMTFWQLFRGKIWPFETISQMDVKNMAEHI
                     MAFFNVRLNEIKKRRLEYMLGAFFLRKSQKHYVVLNEKKRRLISDNLLFQRFCQVMEP
                     VFPNYFQVEDELGALFLVLMTREEYYSDPKIRKKIFDFHQQAKTPPFTALSEAKAALS
                     LYQEEQGLPAENLTFEAENYLFSSHFFAYLFPNAKETIDGNSSDFINHLVIENKELKQ
                     WLVHFFESRHKHPNHLAFKNHAFLMGRYLTVFKTLGAFTPQLPKITILLMTDFPLFEE
                     QLLEEGLRTFFRNEYQLIFLPTDYRGREVDLLISTSKVHRKPWADLDYFIVTEELKLI
                     DYIQLSQKFEMIQKQKLLKQ"
     gene            3825..4046
                     /locus_tag="ACG842_001364"
     CDS             3825..4046
                     /locus_tag="ACG842_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HFQ0247917.1"
                     /translation="MTFEEILPELKKGAKIIRKGWSGFELYVVLVSDDEFDGCPVTPY
                     LLIKTSDEGFSSFAPTVCDILADDWAIVE"
     gene            4043..4804
                     /locus_tag="ACG842_001365"
     CDS             4043..4804
                     /locus_tag="ACG842_001365"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HFQ0247918.1"
                     /translation="MKNQYPDLAKQVVFITGAASGIGAAQARAFLEQDAFVFGLDQKF
                     GKMEEISLRFPETFAYALGDVRQMKELQQAVSKCQNHFGEVTILLNTAGILDAYKPLM
                     ETDESLWDLIYETNVKSMYQLTKIILPDMIRQKSGTIINMASIAGLIAGGGGIAYTSA
                     KHAIVGFTKQLALDYAGQGIRVKGIAPGAIQTPMNAADFEGKGEMAEWVANETPVKRW
                     AQPEEVAELTLFLASPQASYIQGTIIPIDGGWLLK"
     gene            5184..5693
                     /locus_tag="ACG842_001366"
     CDS             5184..5693
                     /locus_tag="ACG842_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HFQ0247919.1"
                     /translation="MEKNNTRSAKRWTTTDVTRMALVTGLYVAVTLVLSVFSFGVIQI
                     RLSEMFNYLSLYNKRYIVAVTAGVALANIASPLGLIDVAVGSVSTLLVLLINYKITSR
                     IKNMKIKMAVTACVFAFSMFTVAGQLTILYQLPFFLNWWVIALGELASMIVGGIIIYW
                     IGKKVDLSK"
     gene            complement(5768..6307)
                     /locus_tag="ACG842_001367"
     CDS             complement(5768..6307)
                     /locus_tag="ACG842_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HFQ0247920.1"
                     /translation="MNRTLKELILAAEFAAIISVLSQMTIPFGLVPLTGQTFAIGLTA
                     TFLGRKLGLISVGVYLLLGLIGLPVFSGMTGGIAVLFGPTGGYLIGFLLQTWITGWMI
                     ERTDAHYLYAIFANLMGSLAALICGTIWLKISGDLTFTTAFASGLLPFLLPEAIKATG
                     AAYIGILMKKRLNRFLHTN"
     gene            complement(6447..7058)
                     /gene="rpsD"
                     /locus_tag="ACG842_001368"
     CDS             complement(6447..7058)
                     /gene="rpsD"
                     /locus_tag="ACG842_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HFQ0247921.1"
                     /translation="MSRYTGPSWKISRRLGISLSGTGKELARRPYAPGQHGPNSRGKQ
                     SEYGMQLTEKQKLRHMYGMNERQFRNLFVKASKIKEGKHGVNFMILLEQRLDNVVYRL
                     GLATTRRQARQLVNHGHILVDGKRVDIPSYHVEVGQVISVREKSQNIVTVKEAVEATV
                     GRPAFVSFDAEKLEGSLTRLPERDELYPEIDEALVVEYYNQSL"
     gene            7320..8171
                     /locus_tag="ACG842_001369"
     CDS             7320..8171
                     /locus_tag="ACG842_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015499 - formate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015724 - formate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HFQ0247922.1"
                     /translation="MRGYVQMEKSEGSLRRKSMNPVSPLFEKIDASIEKKANLFENSF
                     SRYAVRAVLACLFLTLGTAVAFAIAMKGEDIAHGLGKILYAFMFSWGLVMILYMNAEL
                     GTSNMLYMTVGVFRQRLALSKAAKILFACIFFNMIGGILCGFLISMTTPFQDLTLDNF
                     MLESIAVKLAKPSATILVEGMFANIVVNTAVLISMRMKEDVGKVITVVFIIFIFAFLG
                     YEHVIANFPAFTLTYFASHGQMDGMTVSNVLHNLFFALAGNYIGGGLVMGLGYAWLNQ
                     SKSSYVD"
     gene            8199..8834
                     /locus_tag="ACG842_001370"
     CDS             8199..8834
                     /locus_tag="ACG842_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HFQ0247923.1"
                     /translation="MEVLVFPKNEEKALQMEKYMKNLFSFAGVPKPERALLQQPLLDE
                     SKNLSISEVLAFVHYYYEQIEREYQYVAIDLVQKNIKRLTFEDLVVLLPLVQKKEWWD
                     SIDSWRKVYSTWCKAYPEQREEVFQLFEKKEDFWLRRIAITLQLGYKQQTDTGLLIRA
                     IDEDRQTKEFFIQKAIGWALRDYSKTDADWVKRQLTTDLSKLAVREASKYL"
     gene            8854..9627
                     /locus_tag="ACG842_001371"
     CDS             8854..9627
                     /locus_tag="ACG842_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFQ0247924.1"
                     /translation="MRRKLFAFDIDGTLLDSNKQALDSTREALEKLRKAGHLVTIATG
                     RSRFHAQEIIRDLDFTNYILCNGAAGFLDHEQVYKNLLDRDQLDAFVQEAHHEQIDTA
                     FVSLDNIKRSSSNDVTVMEEAMHSFGAVLPELDQFFAEEQEIYQALAFFDQTYDGKFS
                     SYDKIRFVRWHENSVDVVPKNGSKAATILHLAEQVGIPHEDIISFGDGQNDREMLRMS
                     GIGIAMGNAVPEVQAEAKMVTDTNDQDGIWKALKELSVI"
     gene            10035..10832
                     /locus_tag="ACG842_001372"
     CDS             10035..10832
                     /locus_tag="ACG842_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HFQ0247925.1"
                     /translation="MRVLFIGDVVGSMGREMITEYLPRLKKKYRPQVTIVNGENAASG
                     RGITEKIYKKFLQDGVDVVTMGNHTWDNRGIFEFVNEAKKMVRPANFPEGTPGQGMVF
                     VKVNQVELAVINMQARSFMVDLDDPFRKMNELVEEARKRTPIIFVDFHGETTSEKQAM
                     GWFLDGKVSAVVGTHTHVQTNDARILPRGTAYLTDVGMTGPYDGILGMRREPVIEKFL
                     TALPKRFEVVEQGRSILSGCILELDDTTGHAKEIQLIQINDDRPFME"
     gene            10810..11223
                     /locus_tag="ACG842_001373"
     CDS             10810..11223
                     /locus_tag="ACG842_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HFQ0247926.1"
                     /translation="MIDRSWSRKRGSFLQQEPEIQKEVRKLTKLLRENETIIRYKELE
                     EKIQQNQYLAELREKIKQAQKDAVHFAHYDKPAAEKEAIKQADQFMQEFDQHPLVVAY
                     RKQLLEADDLLHHLTTMIQEEINGQIEEEKHASKN"
     gene            11207..13852
                     /gene="mutS"
                     /locus_tag="ACG842_001374"
     CDS             11207..13852
                     /gene="mutS"
                     /locus_tag="ACG842_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HFQ0247927.1"
                     /translation="MPQKTKNTPMMEQYLSIKAQYQDAFLFYRLGDFYELFYEDAIKA
                     SQLLELTLTSRNRNAEEPIPMCGVPHHAAQGYIDALIEKGYKVAICEQVEDPKTTKGM
                     VKREVIQLVTPGTVMDSKGLSAKDNNFLTAVVSQGNEFGFAYADLSTGELKTARLHDE
                     EAVLNEASALQTKELVLGSEITDTLREQLSGRLGLVFSQQNEMEENAEFSFLTSELVD
                     PLEKEATGKLLTYLSVTQKRSLAHIQKAVEYQPDHFLKMDYYSKFNLELSQSIRTGKK
                     HGTLLWLLDETKTAMGGRLLKQWLDRPLIQAKQIKARQEMVASLLDSYFERIDLQSAL
                     TKVYDLERLAGRVAFGNVNGRDLIQLKTSLEQVPMIRGLIEGIDQGQWQSLLSEMQPM
                     DHLVQLIDQAIQDDPPLQITEGNIIKDGFNEQLDMYRSAMRNGKKWLAELEAKERQET
                     GIKNLKIGFNRVFGYYIEITKANLGNAELEKYERKQTLANAERFITPELKELETQILE
                     AEEKSVDLEYQLFLAVREEVKKAIQPLQVLAKAISAVDVLQSFATISERYQYVRPELV
                     SDKHQLLIKDGRHPVVEKVLGHQEYIPNSVEMAEDEMILLITGPNMSGKSTYMRQLAL
                     TVLMAQMGCFVPAKQAVLPIFDRIFTRIGASDDLIAGQSTFMVEMMEANQALRYATPN
                     SLILFDELGRGTATYDGMALAQAIIEYIHRHVQAKTLFSTHYHELTVLEKELPQLKNV
                     HVGAVEKDGEVVFLHKMMDGPADKSYGIHVAKIAGLPSNLLERAADILHTLENGDNGH
                     HTVPAEVEEKTKTAKQEQIKPPAEKVNQDHIQDRVKEENQQLSLFSELSENETEVIES
                     LKQINLLEMTPMDALNTLYELQKQL"
     gene            13870..15978
                     /gene="mutL"
                     /locus_tag="ACG842_001375"
     CDS             13870..15978
                     /gene="mutL"
                     /locus_tag="ACG842_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032300 - mismatch repair complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HFQ0247928.1"
                     /translation="MAKIQELSERLANQIAAGEVVERPASVVKELVENAIDAGSTQID
                     ILVEEAGLRTIQVIDNGEGILADDVENAFKRHATSKIHNRDDLFRIRTLGFRGEALPS
                     IASVSEMTLETANQEEKQGTYLSLKGGEVIEHRPAPLRQGTKITVSNLFFNTPARLKY
                     VKTLHTELANIGDIVNRLALSHPSIAFRLVHDGNKMLATAGNGDLKQTIAGIYGLSTA
                     KKMLKIEGKDLDFELFGYVSLPEVTRASRNYLSTIINGRFIKNFSLNKAIVAGYGSKL
                     MVGRFPIAVLEIKMDPLLVDVNVHPTKQEVRLSKEKELTELISNAIQKALSQENLIPS
                     AADNLRFKKKVPDQPKTEQIEIDLSYAFEEEKPKKPSSLNFDRETGTFFVEELKNTFV
                     DRIVDKSVENVENYSGFSEENTTYPEKTAKTVDKFGENVDNFVEKTVEKSEKKEFPIV
                     DNMEKTNEEQADKSVEEDEKIYEEELTLHPEFDFTQGNDKTLEQAIKKLETEKPVQRF
                     PQLEYFGQMHGTYLFAQSSDGLYIIDQHAAQERIKYEYFREKIGEVSDDLQELLVPFV
                     LDYPNSDALKLKEQKHLLEDAGIYLEEFGQNSFIVRAHPTWYPNGEEESIIREMIDML
                     LTTGSVSVKKFREATAIMMSCKRSIKANHYLNEAQARVLLKDLARCENPFNCPHGRPV
                     LIHFTNSDMERMFKRIQDPH"
     gene            16000..16560
                     /locus_tag="ACG842_001376"
     CDS             16000..16560
                     /locus_tag="ACG842_001376"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase"
                     /protein_id="HFQ0247929.1"
                     /translation="MKIVLASQSPRRKELLAHIVSEFEIEPADIDETPFPDEQPVDYV
                     RRMAEEKARTVWEQDEKEDHLVIASDTTVVLNQEIMGKPEDLAEAEFMLKKLSGETHT
                     VYTAVVLKTKEREERILAEAHVTFYPLTDEEIKRYLETGDYADKAGAYGIQNAASVFV
                     KEISGDYYSIVGFPIGAVNQALKTFI"
     gene            complement(16645..18633)
                     /gene="treP"
                     /locus_tag="ACG842_001377"
     CDS             complement(16645..18633)
                     /gene="treP"
                     /locus_tag="ACG842_001377"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015574 - trehalose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015771 - trehalose transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HFQ0247930.1"
                     /translation="MAKYQADAEKLLHDIGGKENIAAVSHCATRMRFVLNDPGKADEK
                     AIEDIPSVKGMFTNAGQFQVIIGNDVSTFYNDFVAVSGVEGVSKEQGKAAAKQNLHPV
                     QRAIAVLAEIFTPLIPAIIVGGLILGFRNVLEGIQFESLGGTIVEHSKFWNGVNAFLW
                     LPGEAIFHFLPVGITWSIAKKMGTTQILGIVLGITLVSPQLLNAYSVASTAAADIPFW
                     DFGFAQVQMIGYQAQVIPAMLAGFMLAYLEIFFRKYIPQSISMIFVPLFSLLPTVLAA
                     HVILGPIGWTVGSWISNIVNTGLTSSISWLFSAVFGFLYAPLVITGLHHMTNAIDMQL
                     IADFGSTNLWPMIALSNIAQGSAVLAIIFLHRGNKKEEQISIPAMISCYLGVTEPAMF
                     GINLKYVYPFVAAMVGSGLAGMFANLMGVRANAIGVGGLPGILAIQAETWVPFIIAMI
                     IAVIIPFGLTIIFRRQGILNKIDPAVPENAADVQLQTANGATATPQSFEPVSATGTAV
                     ATKETLFAVAAGTIKEITEVNDPVFSQKMMGDGYAVEPSNGKVYAPVNGKVTSVFETK
                     HAIGILSNEGLEVLVHMGLDTVELKGVPFNVFVKEGYLVTPETLIAEMDLPEIEQAGK
                     KTDIIVALTNNEKVAGLSLDQSGLVRPGEAVGKAEVKS"
     gene            18856..19569
                     /gene="treR"
                     /locus_tag="ACG842_001378"
     CDS             18856..19569
                     /gene="treR"
                     /locus_tag="ACG842_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor"
                     /protein_id="HFQ0247931.1"
                     /translation="MNKFNEIFLDLEQKILAKEYPPHSLLPSENQLIKIYGVSRETIR
                     KALNLLTTEGYIQKKQGKGSIVLDRNRFDFPISGLTSYKELQETQRIPSETIIHTLEE
                     TEVTKALNEITGWEIGAPVWHLIRERKINGEVVILDTDYLLKEIVPILTPAQAQGSIY
                     EYFENELSLTIAYAQKEITVEEVTEIMKTAMDLHDDTYAVVVRSLVHLEDTRCFEYSE
                     SVHRLDKFRFVEFARRRKV"
     gene            19646..20092
                     /gene="msrB"
                     /locus_tag="ACG842_001379"
     CDS             19646..20092
                     /gene="msrB"
                     /locus_tag="ACG842_001379"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HFQ0247932.1"
                     /translation="MDKPIRPEKEELKEKLSPMAYAVTQENATERPFSGKYDDFYEKG
                     IYVDIVSGEPLFSSAEKYDAGCGWPSFSKPITKRQVREKADFSHGMHRIEVRSKEADS
                     HLGHVFTDGPVDQGGLRYCINSASLKFIPYEKMDELGYGEFKSLVE"
     gene            20262..20864
                     /gene="ruvA"
                     /locus_tag="ACG842_001380"
     CDS             20262..20864
                     /gene="ruvA"
                     /locus_tag="ACG842_001380"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HFQ0247933.1"
                     /translation="MYEYLTGVVTFINPYYIVVETNGIGYQIALGNPYRYSSKLNQKI
                     KIYVHQVIREDAHLLYGFDSLEEKQLFLRLVSVSGIGPKSALAIMASEDHSGLIQAVE
                     SSDVTYLTKFPGVGKKTAQQMILDLKGKFGELSIDTPFSLFDEANTKDATALSEAMEA
                     LSALGYSDREIKRVEKQLKEVDNQTTDEYLRQALKLMMKK"
     gene            20877..21878
                     /gene="ruvB"
                     /locus_tag="ACG842_001381"
     CDS             20877..21878
                     /gene="ruvB"
                     /locus_tag="ACG842_001381"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HFQ0247934.1"
                     /translation="MTDEYLLSPESGENELSLEKSLRPQLLSQYIGQRKVKQELQIYI
                     EAARNREEPLDHVLLYGPPGLGKTTMAMVIANEMTVNIRTTSGPAIERPGDLVAILNE
                     LEAGDVLFIDEIHRLPRVVEEMLYSAMEDFYVDIMVGQGPTAHPVHFPLPPFTLIGAT
                     TRAGMLSAPLRDRFGIISHMEYYDEQDLQEIVLRSSDIFQTEIIEEGALEIARRSRGT
                     PRIANRLLKRVRDFAQVQGDGKIDRPIADQALTLLQVDQAGLDYVDQKLLRTMIELYG
                     GGPVGLSTLSVNIGEERETVEDMYEPYLIQKGFLKRTPRGRIATAYAYEHFGYDYHI"
     gene            21907..22485
                     /locus_tag="ACG842_001382"
     CDS             21907..22485
                     /locus_tag="ACG842_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFQ0247935.1"
                     /translation="MSDTTRTKQLIKNVLIDLCQTQSFRKISVQKIANEAKINRQTFY
                     YHFTDKYDLLRWVYCEDALHYLDPQCLSLDNWEEQALKMLKAIAEKKEFYANTVLSDP
                     EILQKEFSQLIQKSFIKIFEQMDEERQLSARDKLFYARFFSYGCSGVLVNWITNEYEE
                     TPMEIAMQLFRLAKDTELYSYRLYAKEELEEQ"
     gene            22537..23019
                     /locus_tag="ACG842_001383"
     CDS             22537..23019
                     /locus_tag="ACG842_001383"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase"
                     /protein_id="HFQ0247936.1"
                     /translation="MTKILFVCLGNICRSPMAEGLLRKRIALEGREAEFFVDSAATST
                     YEVGKTPHPGTKKILNQEKVDMTNMVARQITPHDFETFDWIIGMDQENVEELKRRAPR
                     SAQGKIHLFLSSIPGKEKENVPDPYYTNNFDETYHLITEGLNHWWNIWIDENKITNNL
                     "
     gene            23136..23510
                     /locus_tag="ACG842_001384"
     CDS             23136..23510
                     /locus_tag="ACG842_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0247937.1"
                     /translation="MNEETVSPMKKLTKLVTHTSYQLFDSFVNDTEEHAKNVRLQGKF
                     ILELQKAATEHSLVILQVRANRLSSKYETISGWVATKTVEKDRVMIRLQNEEQQIRMV
                     PVEQIEKITILSPAGEQERLSK"
     gene            complement(23885..>24022)
                     /locus_tag="ACG842_001385"
     CDS             complement(23885..>24022)
                     /locus_tag="ACG842_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HFQ0247938.1"
                     /translation="EQFQNEESLERFLVSIFDTYNQKFLNRSHKGFQQVTDTLVSMFT
                     E"
BASE COUNT         7841 a         4314 c         5150 g         6717 t
ORIGIN      
        1 ataaaaaaac ttggtacgca atcgctgtac caagtttttt tatttagctt ttgattaagc
       61 cataaataat aatttatata agaatactgc tacgacacca gcacagatag gtgctacaac
      121 tggtacccat gcatatcccc attgagaaga acctttatgt ttcaatggaa gaagtgcgtg
      181 tacaagacgt ggagcaaagt cgcgggctgg gtttagtcca ggacctgtag gaccaccaag
      241 tgaagcaact aatgtccata caaggaaacc aagtgctaag tgagctgtac caagattatc
      301 ttggaagaat ggtgcttttg taatacctag ggcaccaaag aataaaacga atgaaccaaa
      361 gaattcattg acgaaaccat tgaatttaga accaacgctg ttgattgttg agaatgtacc
      421 taacacatgg tttacgtctt ctgtttggtc gtagtaaggt ttgtaacaag ctactacgat
      481 caattgtcca atcgctgcac caatcaattg tgcaatgata taaggaacaa cttcagccca
      541 agggaataga ccattagatg ctaaagcaat tgtaaatgct gggttgatgt ggttaccgct
      601 gattccacca aaaatcattg ctggcatcat aaccccgaat ccatacccga cagcaatcag
      661 catccagtca ctgccatatc cttttgtacc ttttaaatct acgtttgcta cagcaccgtt
      721 acctagaacg attagtaatg ctgtaccaat catttccgcc gcaagacggg tcattagtga
      781 gtaatccata aatacctcca aaagttataa aaatgcaaaa aaaaaagaaa catcaacacg
      841 aaaagcatcg gtgcagtgtt tcgtgtcgga aggatttctg cagtcttaac aaatgtgaat
      901 aaaaaaatga attccattac ttataagaaa ccagaccaac agtaaaactc caactctttt
      961 taaaagttct ccacaaatca ccaagcttaa ctataaatat accaaaaaaa aaacgttttt
     1021 tttctcaaaa attgtatctt agaaacagca tgttttgaaa ctttcagaaa aggtttgtta
     1081 gtaaaatgaa agctatacgt aaggagtgtt gtttgatgaa attttttcaa gagtttttca
     1141 ttattatttt cttttctgta ttaggagaag gcatacggat ttttgctcat ttacctattc
     1201 ctggaagtat cttaggtatt ctttttttat ttttagcatt tgaaatgcat cttgttgatc
     1261 ctgaaaaaat cggagcaact ggtgattttt tattgaataa tctagccatt ttatttgttc
     1321 ctgctggtgt aggtttattg gaatattttg atgatattgc ttcgatatgg cctgttttac
     1381 tgggagcagt cgtcgtgtgt tccatcgtca cgatggttgc agcgggaaaa acggcagaag
     1441 gtgtagaagc tttgctgggc tatgtccgta agaaaaaagc agaaagggta tctgtaaacg
     1501 aaatggagga gaatcaaatt gactgagttt tttgtgaatc cattatttgg cttaagtttg
     1561 actttaggag tgtacgtatt atctgacaaa tttttgcgga aatataaact gcctgttttg
     1621 aatcctctcg tattatcgat tgttgttatt atcttatttt tgcatttttc tgggatcagc
     1681 tataaagact ataatatcgg gggcaatttt ttgactatgc tgattactcc tgcgacagtg
     1741 gcacttgcca tccctcttta taaaaacttc cgtttactga aagagaattt ttatcctgta
     1801 atggctgcga ttttagcagg aatagtcgca aattgcttgg ttagtgtagg gttcggttac
     1861 ttcttctctc ttcataaaga gatggtgatc tcattactac ctaaatctgt aacgacggct
     1921 atttctgtcg atttatctca tacgatgggc ggggtgaatg ccgtgacact tgctattgtt
     1981 gtctctacag gtatttttgg ttcgctgatc gccactcata ttttccgcct attcaagatc
     2041 gaaagtccag ttgctcgcgg tgtggcgtta ggaagtactt ctcatgctat tggtacagca
     2101 aaagcaatcg agattggtga aatagaagga atcatttccg gacttgcgat atgcgtcaac
     2161 ggaatattga ccgttttatt gctaccgtta ttctttcagc cttttgctgg cttgttttga
     2221 tgcgaaaacc actattttat gctacaataa ccaaaacgtt cgagcataaa aaaggaggga
     2281 aagacgtgta tttgtcttcc gagtatctta aacaatttga taaagatcta tattataaga
     2341 ttctcctcct agaaagtttt gaggatcaag cttggcatac cgctgcacaa ttagcgcaag
     2401 ttgttcagct ggatgcccgc agcgtgtcga aatacctgaa cgagctgtct aaaaactatc
     2461 agcagtttag cggtaagaca caccctttgt ttacaaaaaa tcaccgttct ggttataact
     2521 tttatgatac gctcgattca attgaacatg agcgtttttt gatctatctc gtgcaaagta
     2581 cgctgaaatt ccagttgctg cacgacattt tttttgaaga attccataca atgtaccaat
     2641 ttgcgcaaaa gcattacatt agtgaatcta ctgctcatcg aaaaatcaat gaatggaaac
     2701 agcaactgca aacttacgga attcggctgc aacgaggtac atatatcgct caaggcgaag
     2761 aagaaatcat tcgactttac ttgcatatga cattctggca gttgtttcga gggaaaatct
     2821 ggccatttga aacgatttct caaatggacg taaagaatat ggcagaacat atcatggctt
     2881 tttttaatgt tcgtctgaac gagatcaaaa aaagacgatt ggaatatatg ctaggtgcct
     2941 tttttttaag aaaatcacag aagcattatg ttgtattgaa tgagaaaaaa agacggctga
     3001 tttctgacaa tctgttgttc cagcgatttt gccaggtcat ggagccggta tttcctaatt
     3061 attttcaagt agaagacgag ttaggcgcat tatttcttgt actgatgact cgtgaagaat
     3121 attatagtga tcctaagata cggaagaaaa tttttgattt tcatcaacaa gcaaaaacac
     3181 cgccctttac agccctttca gaagcaaaag ccgctctttc tctttatcag gaagagcagg
     3241 ggctgccagc agaaaatcta accttcgagg cagaaaacta tttattcagc agtcattttt
     3301 tcgcctatct ttttcctaat gcgaaagaaa cgattgatgg caatagtagt gattttatta
     3361 atcatttagt tattgaaaac aaggaattga aacaatggct cgtacatttt tttgaaagta
     3421 gacacaaaca tcccaatcat ctggcattta aaaatcatgc gtttttgatg gggcggtatc
     3481 tgactgtgtt taaaacatta ggagccttta ctccacagct tccaaagatc acgatccttt
     3541 taatgacaga tttcccatta tttgaagagc aactgttaga ggagggatta cggacatttt
     3601 tccgtaatga gtatcagctg atttttctgc caacagatta tcgggggaga gaagttgatc
     3661 ttctgatttc gacaagtaaa gtccaccgca aaccatgggc ggatcttgac tattttattg
     3721 tgactgaaga actaaaatta attgattaca tccaactatc tcaaaaattt gaaatgattc
     3781 aaaaacaaaa actattaaaa caataacaaa gaaggagaaa aaagatgaca tttgaagaaa
     3841 ttttaccaga attgaaaaaa ggtgcaaaga ttattcgtaa aggatggagt ggatttgaac
     3901 tttatgttgt tctcgtatcc gatgatgaat ttgatggctg tccggtgacg ccgtacttat
     3961 tgatcaaaac aagtgacgaa ggtttttcca gcttcgctcc tacagtttgt gatattttag
     4021 cagatgattg ggcgatcgta gaatgaaaaa ccagtatcca gatctagcta aacaggttgt
     4081 atttatcaca ggagctgctt ctggaattgg tgctgcgcaa gcccgagcgt ttcttgaaca
     4141 agatgctttt gtatttggat tagatcaaaa gttcggtaaa atggaagaaa tttctttgcg
     4201 atttcctgaa acttttgcct acgcactagg tgatgttcga caaatgaaag aactccaaca
     4261 agcggtatcg aaatgccaaa atcattttgg tgaagttacg attttactga atacagcggg
     4321 gatattggat gcctacaaac ccttgatgga aactgacgaa tcgctgtggg atctgattta
     4381 tgagacgaat gtgaaaagca tgtatcagct gacaaaaatc atccttcctg atatgatccg
     4441 ccaaaaaagc ggaacgatca tcaatatggc ttcgattgct ggcttgattg ctggtggagg
     4501 tgggatcgct tatacaagcg cgaaacatgc gattgtcgga tttaccaaac aactcgctct
     4561 ggattacgca ggacagggta tccgagtaaa aggaattgct ccaggagcca tccaaacacc
     4621 aatgaatgcg gctgattttg aagggaaagg tgagatggca gagtgggtgg caaatgaaac
     4681 accagtcaag cgatgggcac agccggaaga agtagctgag ctgacgttat ttcttgcctc
     4741 tcctcaagca agctacatac aaggaacgat catcccaatc gacggcggat ggttgttaaa
     4801 ataaccttgt gtttttttca agcttctgta tgataaaact aaagagagaa acaacggttg
     4861 atgataaaaa aagtcgatga ctgttcccga cattcgatgc cgaacagcca gttcaaaaaa
     4921 ttctttttaa taatcgttaa tattcgaaac taagcggctt ttcacgacct cttgatataa
     4981 acgatattca aaagcttgca actgcggtaa caagtccagc caaatgaaaa atataaggag
     5041 ctatttttat ttgaccattc ttattacttg ttgcagggca gctttttcac taccttctgc
     5101 gacacttcag ttggaatttt cttcccatcc tttggtcgcg gtcattacca agataaaatt
     5161 atcttgcaaa ggagaaatag aacatggaaa aaaataatac ccgttcagcc aaacgctgga
     5221 caacgacaga tgtgactcgg atggcgctag tcactggttt atatgtggca gtgacgctgg
     5281 ttttgtctgt ctttagcttt ggagtgattc agattcgtct ctcggagatg ttcaactatc
     5341 tgtccttgta taacaaaaga tacatcgtcg cggtaacagc gggagtagct ttggctaata
     5401 ttgcttctcc tcttggactg atagatgttg cagtaggaag cgtcagcacg ctgcttgtat
     5461 tgcttatcaa ctataaaatc actagtcgca tcaaaaacat gaaaatcaaa atggccgtga
     5521 cagcatgcgt atttgctttt tccatgttta cagtagctgg tcagctaacg attttatatc
     5581 aactaccttt tttcttgaat tggtgggtga ttgctttagg agaattggca tcaatgattg
     5641 tcggtgggat aattatttat tggatcggta aaaaagtgga tttgtcaaaa taaagatctg
     5701 tttaatttaa aatagggcag catggaagag actgaatgtt gagtcttctt ccatgctgct
     5761 tttttgatta atttgtatga agaaatcgat ttagccgttt tttcatcaag ataccgatat
     5821 aagctgctcc agttgccttg attgcttccg gcagtaaaaa cggcagtagt ccactggcaa
     5881 atgcagttgt aaaagtaaga tcaccactta ttttcagcca gatcgttccg cagatcaatg
     5941 cagcaagaga gcccattaga ttggcaaaga ttgcatataa gtaatgagca tccgttcttt
     6001 cgatcatcca accagttatc cacgtttgta agagaaatcc aatcaaatat ccgccagttg
     6061 gcccaaataa aacagctata cctccagtca tccctgaaaa tacaggaaga ccaatcaagc
     6121 caagtaaaag ataaactcca acagatatca atcctagttt tcttccgaga aaagttgctg
     6181 ttaatccaat agcaaatgtt tgccctgtca aaggaaccaa accaaaagga atggtcatct
     6241 gagaaaggac agaaataatc gcggcaaatt ccgcagcaag tatcaattct ttcaatgttc
     6301 ggttcataac gccctctttt cgttaactta atatttttat ttggttaacg aaattgtaaa
     6361 acaaagtgat tactttgtaa atagaaaata taaaaaaaga ccagctatcc aatagatagc
     6421 ggtctttttc aagaaagctt ttgaaattat aagctttggt tgtagtattc aacgacaaga
     6481 gcttcgtcga tttctggata tagttcgtca cgttctggta aacgagtcaa tgaaccttcc
     6541 aatttttcag cgtcaaagct tacgaatgct gggcgtccaa cagttgcttc tacagcttct
     6601 tttactgtta cgatgttttg tgatttttca cgaactgaga tcacttgacc cacttctacg
     6661 tggtatgaag ggatatcaac gcgtttgcca tcaactaaaa tgtgaccatg gttaactaat
     6721 tgacgtgctt gacgacgagt cgttgcaagt cctaaacggt aaacaacatt gtctaaacgt
     6781 tgttctagta agatcatgaa gttaacacca tgtttacctt ctttgatttt gctagctttt
     6841 acgaacaagt tacggaattg acgttcgttc attccgtaca tgtgacgaag tttttgtttt
     6901 tcagttaatt gcatgccgta ttctgattgt ttgccacggc tattaggtcc atgttgacct
     6961 ggtgcgtaag ggcgacgtgc taattctttt cctgtgcctg acaatgagat gcctaagcga
     7021 cgagaaattt tccatgatgg tcctgtataa cgagacatta aaatttcctc caatgaatat
     7081 aagtttttgg agtaaaataa tccgttgaaa aattcataat catatagttt gttcttcaat
     7141 cttcaccttt gcagccgcgg ttacacaatc gaacccgaga cggggcaaca aactagggat
     7201 gagttattat ttttctgctg cattatttta cacaaattac agtatatgat agatcatcca
     7261 cgaatgtcaa gataattagt acataaaaac gctttacaaa cctaggctgt ctttataaaa
     7321 tgagagggta tgtgcaaatg gaaaaaagcg aaggaagttt aaggagaaaa tcaatgaatc
     7381 cggtatcacc attatttgag aaaatcgatg cttcaattga aaagaaggcg aatctttttg
     7441 aaaacagctt ttcacgttac gctgttcgag cggttctcgc ctgtttgttt ttgactttag
     7501 gaacggctgt ggcatttgcg atcgcgatga agggggaaga tatcgcgcat ggtctaggaa
     7561 agattttata cgcatttatg ttcagctggg gtctggtcat gattctatac atgaacgccg
     7621 aattaggaac atcgaatatg ctttatatga ccgtgggtgt ttttcggcag agacttgctt
     7681 taagtaaggc agccaagatt ctatttgcct gtattttttt caatatgatt ggcggtatat
     7741 tatgtggatt tctaatttct atgactacgc catttcaaga tttaacgttg gacaacttta
     7801 tgttagaatc aatagcagta aaactagcaa aaccttcagc aacgattttg gtggaaggga
     7861 tgtttgcgaa tatcgttgtc aataccgctg tactgatcag catgcggatg aaagaggatg
     7921 tcggaaaagt gatcacagtc gtattcatca ttttcatttt tgctttttta ggatatgaac
     7981 acgtgatagc gaattttcct gcctttacgc ttacttactt tgcttctcac ggacagatgg
     8041 atggaatgac agtcagcaat gtgttacata atttgttttt tgctttggca ggaaattata
     8101 ttggtggcgg tttagtaatg ggactgggat atgcttggct gaatcagtca aaatcatcct
     8161 atgtggacta aaaagtcgtt cattaaaggg gaagtgccat ggaagtttta gtttttccaa
     8221 aaaatgagga aaaagcgtta caaatggaaa agtacatgaa aaatcttttt tcctttgctg
     8281 gagtacctaa gccggaacgc gctcttttac aacaaccgtt actggatgag agcaaaaatt
     8341 tatctatttc tgaagtttta gcttttgttc attattatta tgaacaaatc gaaagagagt
     8401 atcagtatgt agcgatcgat ttagtccaga aaaacatcaa acggcttaca tttgaagact
     8461 tagtcgtatt gttgccacta gttcagaaaa aagaatggtg ggacagcatc gactcttgga
     8521 gaaaagtata cagcacatgg tgtaaagcct acccagagca gcgtgaagaa gtctttcaat
     8581 tgtttgaaaa aaaggaagat ttttggctac gtcgaatagc aatcacgttg caattaggtt
     8641 ataaacagca aacagacaca ggactattga tccgagcaat agacgaagat cgtcaaacaa
     8701 aggaattctt tatccaaaaa gcaattggtt gggcattacg cgattatagt aagaccgatg
     8761 ccgattgggt aaaaagacag ctgacaacag atttgagtaa gttagctgtt cgagaggcaa
     8821 gtaagtattt ataagaaagc gagacgacaa gagatgagac gcaaattatt tgcttttgat
     8881 attgatggaa cgttgttaga ttcaaacaaa caagctttag atagcacaag agaagcattg
     8941 gaaaaattac gcaaagccgg tcatctggtc acgattgcta cagggcggag ccgatttcat
     9001 gcgcaagaga tcattcgcga tcttgatttt acgaactaca ttttatgtaa tggagcggcc
     9061 ggttttttag accatgaaca agtatataag aatttactag atcgcgacca gttggatgcg
     9121 ttcgttcaag aagcgcatca tgagcagatc gatacagctt ttgtcagctt agacaatatc
     9181 aagcgttctt cttccaatga tgtaacggtg atggaagaag cgatgcattc tttcggtgct
     9241 gtcttgcctg aattagatca gttttttgca gaggaacaag aaatatacca agcgttagca
     9301 tttttcgatc aaacatatga tgggaagttc tcttcatatg ataaaattcg atttgtccgc
     9361 tggcatgaga acagtgtaga tgtcgtacct aaaaatggtt ctaaagcagc gacaattctg
     9421 cacttagcag aacaagtagg catccctcat gaagacatca tcagctttgg cgatggacaa
     9481 aacgaccgtg aaatgttgcg catgtcagga attgggatag ctatggggaa tgctgtgcca
     9541 gaagtacaag cagaagctaa gatggtcact gatacaaacg atcaggatgg aatttggaag
     9601 gcattgaaag agttaagcgt gatttaaaag aggttgtgaa aaagctgttc tgcatcaagt
     9661 aataagccca gccaaacaaa aatagctcct cctatttttc gtttttctgg gcttattacc
     9721 gcagatgcaa gctttcgaac accgtttaag gaagaggtcg tgacagaagt ggtcagtttc
     9781 aagaaataag aagggattta cgaaaattgc ttctcaaatt tttgtaaaat tacggcttat
     9841 ttctgaagaa gtagatgaaa caacgtttat tcgggaaatg ggactgtgac aagatgtttg
     9901 tcatagtccc atttttttat tttatttttc aaaactaaga aataccagga aagagttcct
     9961 tttgtttgac cttttctttc ttattgaaaa caactataat atagagtgat gttaaaagag
    10021 aagaggaatt tagtgtgcgg gtattgttta ttggtgatgt agtaggttca atgggacgag
    10081 agatgattac tgaatatctt cctagattga agaagaagta ccgtccgcaa gtaacgattg
    10141 taaatggtga gaatgccgct tctggacgtg gaatcacaga aaaaatctat aaaaaatttt
    10201 tgcaagacgg cgtagatgtc gtgacgatgg ggaatcatac gtgggacaac cgcggaatct
    10261 ttgagtttgt aaatgaagct aaaaagatgg ttcgtccggc aaattttccg gaagggacgc
    10321 ctggacaagg gatggtcttt gtcaaagtga accaagtcga actggcagtg atcaacatgc
    10381 aggctcgttc ttttatggtg gacttggatg atccttttcg taaaatgaat gagctggtag
    10441 aagaagccag aaaacgtacg ccgatcattt ttgtagattt tcatggagaa acaacaagtg
    10501 aaaaacaagc aatgggctgg tttttagatg ggaaagtttc agcagtggtc ggtacacata
    10561 cacacgttca gacaaatgat gcgcgaattt tgccaagagg aacagcgtat ctgacagacg
    10621 ttgggatgac aggtccctat gatggtattt tgggtatgcg cagagaacct gtaatcgaaa
    10681 agtttctgac tgcattacca aaacgttttg aagttgtgga acaaggccga tcgattttaa
    10741 gcggctgtat cttggaattg gatgatacga caggtcatgc aaaagaaatc cagctgattc
    10801 agatcaacga tgatcgaccg ttcatggagt agaaagagag gcagtttttt gcagcaggag
    10861 ccggaaattc aaaaagaagt aaggaaactg acaaaacttt taagagagaa tgaaacgatc
    10921 attcgctaca aagaacttga agaaaagatc cagcaaaacc agtatttagc tgagttgaga
    10981 gaaaaaatca aacaagcgca aaaagatgct gttcattttg cccactatga caaaccagca
    11041 gcagaaaaag aagcaatcaa acaagcggat caattcatgc aagaatttga tcagcatccg
    11101 cttgttgtag cttatagaaa acagctgttg gaagcagatg atctgcttca tcatttaaca
    11161 actatgatac aagaagaaat caatggacaa atagaggagg aaaaacatgc ctcaaaaaac
    11221 taaaaatact cctatgatgg aacaatatct aagcatcaag gcccaatacc aagatgcttt
    11281 tttgttctat cgtttaggag acttttatga actattttac gaagatgcga tcaaagcgtc
    11341 acagcttttg gaattaacgt tgacgagcag aaatcgaaat gcagaagaac ctattcctat
    11401 gtgcggtgta cctcaccatg cagcacaagg atatatcgat gccttgattg aaaaaggcta
    11461 taaagtagcg atttgcgagc aggtagaaga tccaaagacg actaaaggga tggtcaagcg
    11521 cgaagtgatc cagctagtca ctcctggtac agtcatggac agcaaaggat tgtcggcaaa
    11581 agacaacaat tttttaacag ctgtagttag tcaaggaaat gaatttggct ttgcttatgc
    11641 cgatttaagt acgggagaat tgaagacggc tcggttgcat gatgaagaag ctgttttaaa
    11701 tgaagcctct gcattgcaga caaaagaact cgtattaggc agtgagatca ctgataccct
    11761 tcgtgaacag ctaagcgggc gtttagggct agtcttttct caacaaaatg agatggaaga
    11821 aaacgcggaa tttagctttt tgacaagcga actagtcgat ccgctggaaa aagaagcaac
    11881 cggcaaacta ttgacttatt tatcggtgac acaaaagcgg agtttagctc atatccaaaa
    11941 agcagtagaa taccaaccag atcatttttt gaaaatggat tattattcaa aattcaacct
    12001 agaattaagc caatctattc ggactgggaa aaagcacggc actcttctat ggttgttaga
    12061 tgaaacaaaa acagcaatgg gtggccgttt actaaaacaa tggctagacc gtccgcttat
    12121 ccaggcaaaa caaattaaag caagacaaga aatggtcgct tctttgctag acagctattt
    12181 tgaacgtatc gatttgcagt cagcattaac gaaagtctat gatctggaac gcctagcagg
    12241 tcgagtagct ttcggtaatg tcaatgggcg agacttgatc cagctgaaaa cttctttaga
    12301 acaagtgcca atgatcagag ggttgattga aggaatcgat caaggtcaat ggcaaagtct
    12361 gctttcagaa atgcagccga tggaccatct cgtccaattg atcgaccaag ctatccaaga
    12421 tgatccgccg ttacaaataa cggaaggaaa catcatcaaa gatggattca atgagcagct
    12481 cgatatgtac cgttctgcta tgagaaacgg aaagaaatgg ttggcagaac ttgaagcaaa
    12541 agagcgtcaa gaaacaggaa tcaagaactt aaaaatcgga tttaaccgcg ttttcggtta
    12601 ttatatagaa attacaaaag caaatcttgg aaatgcagaa ctggagaagt atgaacgaaa
    12661 acaaacttta gcaaatgcag agcgtttcat cacaccagaa ctaaaagaat tggagacaca
    12721 aatcctcgaa gcagaagaaa aatctgttga tttagaatat cagttgtttt tagctgtgag
    12781 agaagaagtc aaaaaggcta tccagccgct acaagtactt gcaaaagcaa tcagtgcagt
    12841 agatgtgcta caaagttttg caacgatcag cgaacgctat cagtatgttc ggccagaact
    12901 tgtcagtgac aagcatcagc ttctgatcaa agacggacga catcctgtag tagaaaaagt
    12961 attaggacat caggaatata tccctaacag cgtagaaatg gcagaagacg aaatgatctt
    13021 gctgattaca ggaccgaata tgtctggtaa aagtacgtat atgcgacagt tggcattaac
    13081 tgttctgatg gcgcaaatgg gctgtttcgt tccggcaaaa caagcagttt tacctatttt
    13141 tgatcggatt tttacccgaa ttggtgcaag tgacgactta attgctggac aaagtacttt
    13201 tatggtagag atgatggaag ccaatcaagc cctgcgttat gctacgccaa acagtttgat
    13261 tttgtttgac gaactagggc gaggaacagc aacctatgac ggaatggcgt tggcccaagc
    13321 aatcatcgaa tatatccatc gtcatgtaca agcaaaaaca ctcttttcta ctcattatca
    13381 tgaactgacg gtacttgaga aagaacttcc gcagcttaaa aatgtccatg ttggagccgt
    13441 tgaaaaagac ggagaagttg tcttcttgca taaaatgatg gatggacctg cggataaaag
    13501 ctacggcata catgtggcaa agatcgcagg attgccttcc aatctactag aacgagcagc
    13561 agatatcctg catacgctag aaaatggcga taacggacac catactgttc cagctgaagt
    13621 agaagagaaa actaaaacag ccaaacagga gcaaataaaa ccacctgcag aaaaagtaaa
    13681 tcaagaccat atacaagacc gtgtaaaaga agaaaaccaa cagctttctt tattcagtga
    13741 attatccgaa aatgaaacag aagtcattga atcactgaag caaataaatt tactagaaat
    13801 gacgccaatg gatgcactaa acacattata tgaattacaa aaacaactgt aggaagaagg
    13861 cggatatcga tggccaaaat ccaagaatta tctgaacgtt tagctaatca gatcgctgcg
    13921 ggagaagtag tggagcgacc agcttccgtt gtgaaagaat tagtggaaaa tgcaattgac
    13981 gccggcagta ctcagattga tatcttagtt gaagaagcgg gactgcggac gatccaagtg
    14041 attgataacg gagaaggcat tttggcagac gacgtagaga atgccttcaa gcggcatgcg
    14101 acaagtaaga tccataatcg cgacgatctt tttcgcatac gaacgctggg ctttcgaggg
    14161 gaagctttgc caagtattgc ctctgtttct gaaatgactc tggaaacggc gaatcaggaa
    14221 gaaaaacaag gcacctatct gtcgctgaaa ggcggagaag ttattgagca tcgaccagca
    14281 ccactgcgtc aaggaacgaa gattaccgtc agcaatctgt ttttcaatac gccagcccgc
    14341 ttgaaatacg tgaaaacact gcacacagaa ttagcgaaca tcggggacat cgtcaatcga
    14401 ctggcattga gtcatccatc gattgctttt cgtttagtac atgatggaaa caaaatgctg
    14461 gcaactgctg gaaatggcga tctgaaacaa acaattgcgg gaatctacgg attatctacc
    14521 gcaaaaaaaa tgctgaaaat cgaaggaaaa gatctagatt ttgagttatt tggctatgtt
    14581 tcattgcctg aggtgactcg tgccagtagg aattacttat cgacgatcat caatggacgt
    14641 tttatcaaga acttttcctt gaataaagca atcgttgcag gttatgggtc aaaattaatg
    14701 gtcggtcgtt tccctattgc tgtattggaa atcaaaatgg acccgctttt agtagatgta
    14761 aatgttcatc cgacgaagca ggaagtccgg ctgtcgaagg aaaaagaact aacagagcta
    14821 attagtaacg ccattcaaaa agcattgtct caagaaaatc tgattccaag tgcagcggat
    14881 aatctacggt ttaagaaaaa agtacctgat cagcctaaaa cagaacaaat cgaaatcgat
    14941 ttgtcctacg cttttgaaga agaaaaacct aaaaagccaa gtagtttgaa ttttgatcga
    15001 gagacaggta cctttttcgt agaagaacta aagaacacat ttgttgacag aattgttgat
    15061 aagtctgtgg aaaatgttga aaactattct ggtttttctg aagaaaacac cacttatcca
    15121 gaaaaaacag cgaaaacggt ggataagttt ggtgaaaatg ttgataactt tgtagaaaaa
    15181 acagtggaaa aatctgaaaa gaaagaattt cccattgtgg ataacatgga gaaaacaaat
    15241 gaagaacaag cagataaatc cgtagaagag gatgaaaaga tttacgagga agaattgaca
    15301 ctccatcctg aatttgattt cactcaagga aacgacaaaa cattagaaca agccatcaag
    15361 aaattagaaa cggaaaaacc agtacaacgt tttcctcagt tggaatattt tggacagatg
    15421 cacggaacgt atctttttgc ccaaagtagc gatgggttgt acatcattga tcagcatgct
    15481 gcccaagagc gcattaagta tgaatatttc cgggaaaaaa ttggggaagt atctgatgat
    15541 ctgcaagaat tgcttgttcc atttgtgtta gactatccaa acagtgatgc tttgaaatta
    15601 aaagagcaaa aacacttgct agaagatgca gggatttatt tagaagaatt tggtcaaaac
    15661 agctttattg tacgggccca tcctacttgg tatcccaatg gagaagaaga atcgattatt
    15721 cgggagatga tcgatatgct gctgacaact ggaagtgtga gtgtaaaaaa attccgtgaa
    15781 gcaacagcaa tcatgatgag ttgcaaacga tcgattaaag ccaaccatta tttgaatgaa
    15841 gcacaagcac gtgtgctgct gaaggatctg gcacgatgcg aaaatccttt caattgtccc
    15901 catggccgtc cagtattgat tcactttacc aattcggata tggaaaggat gttcaaacga
    15961 atccaagacc cgcattaagt caaagagaag agggaaaaca tgaagattgt attagcatcc
    16021 caatcaccaa gaagaaaaga gttattggct catatcgttt cagaattcga gattgaacct
    16081 gctgatatcg atgaaacgcc atttccagac gaacagcccg tcgattatgt tcgtagaatg
    16141 gctgaagaaa aagcacgaac tgtctgggaa caagatgaaa aagaagacca tttagtgatt
    16201 gccagtgaca cgactgttgt tctcaatcaa gagatcatgg gtaaaccaga agatctggct
    16261 gaggctgaat tcatgctgaa aaaactaagc ggggaaacac atactgtata tacagctgtt
    16321 gttttaaaaa caaaggaacg agaagaacgg atactagcag aagcacatgt cactttttat
    16381 cctttgacag acgaagagat caaacgctac ttggagacgg gggattatgc ggataaggca
    16441 ggggcatacg gcatccaaaa tgctgcgagt gtattcgtca aagaaatttc tggagattat
    16501 tacagtatcg taggatttcc gattggtgct gtcaatcagg cactgaaaac attcatttaa
    16561 acaaaaaagc ggactatctg attttcgatt gaaaatccat gatagcccgt tttttttatt
    16621 gaaaagatta cttatctact ctgactaaga ttttacttca gctttaccga ctgcttcgcc
    16681 cgggcgaaca agtcctgatt ggtcaagaga aagtcctgca actttttcat tattggtcaa
    16741 agcaacaata atgtcagttt tcttgccagc ttgttctatt tctggcaaat ccatttcagc
    16801 aatcagggtc tctggtgtga caaggtatcc ttctttcacg aatacattga aaggaacacc
    16861 ctttagttca acagtatcta atcccatgtg aacaagtact tccagcccct cattagaaag
    16921 aataccaatg gcatgttttg tttcaaagac actcgtcact ttaccgttta ctggtgcata
    16981 gactttgccg tttgacggtt cgacagcata accgtcaccc atcatttttt gtgagaaaac
    17041 tgggtcattt acttcagtta tttccttgat ggtccctgca gcgactgcga ataatgtttc
    17101 ttttgttgca actgccgtac cagttgctga aacaggttcg aaagactgtg gcgttgctgt
    17161 tgcgccattt gctgtttgaa gttgtacatc agcagcattt tcaggaacag caggatcaat
    17221 cttattcaaa attccctgac gtctaaagat gatcgtcaag ccaaatggaa tgatgacagc
    17281 aatgatcatt gcgatgatga aaggtaccca tgtttctgct tggatagcta gtattcctgg
    17341 caaaccgcct actccgatcg cgttagctcg aacacccatc aagttagcaa acatgccagc
    17401 taatccagag ccgaccattg cagcgacgaa aggatagacg tatttcaaat tgattccgaa
    17461 catagcaggt tcagttacgc ctaagtaaca agaaatcatt gctggaatcg agatttgttc
    17521 ttctttttta ttcccacgat gtaagaagat gatggctaag actgcagagc cttgggcgat
    17581 atttgataaa gcaatcatcg gccataaatt cgttgatccg aaatctgcaa tcagctgcat
    17641 atcgatggca ttggtcatat ggtggagacc agtaatcact aaaggagcat acaggaatcc
    17701 gaatacggca ctaaacaacc agctgataga agacgtcaat ccagtattta cgatatttga
    17761 gatccagctt ccgactgtcc agccgatagg acctaaaata acgtgtgcag ctaaaacagt
    17821 tggtaacaat gaaaacaatg gaacaaaaat catcgagatc gactgaggga tatatttgcg
    17881 gaagaagatt tctagatacg ctaacataaa ccctgccagc atggctggaa taacttgcgc
    17941 ttgataacca atcatctgga cttgagcaaa accaaaatcc caaaatggaa tatctgctgc
    18001 ggctgtggaa gctacactat aagcatttaa taattgcgga gaaactaacg taattcctaa
    18061 aacgattcct aatatttgtg tagtacccat cttttttgca atactccaag tgatacctac
    18121 tggaagaaaa tggaaaattg cttcaccggg taaccacaaa aaggcattga caccattcca
    18181 aaacttcgag tgttcaacga ttgttccacc aagactttca aactggatac cttcaagtac
    18241 atttctgaat ccaaggatca atccaccgac aatgattgca ggaatcagtg gtgtaaagat
    18301 ttctgctaaa acggcaatag cacgttgaac gggatgaagg ttttgttttg cagctgcttt
    18361 tccttgttct ttggaaaccc cttcaactcc agatactgca acgaagtcgt tataaaaagt
    18421 agaaacatcg tttcctatga tgacttggaa ctgtcctgca ttcgtgaaca tcccttttac
    18481 gctaggtata tcttcgatcg ctttttcgtc cgcttttcct ggatcattca ggacaaaacg
    18541 catacgagtc gcacaatgac taactgcagc aatattttct tttccgccga tatcatgtaa
    18601 cagcttctcg gcatctgctt gatattttgc catatcaata aacccctttc tcttaacttg
    18661 tctatgcaag ttgtttcttc atttactata ctttttttcg gagagaattg caagcgtttt
    18721 attaaaaagt acgagagaaa atgatatcgt tttaaaaagc ttaaaataag cttttcttaa
    18781 aaataggaac attgaaatac aaaaagagct gtagtatagt gaagaaatga tggaagaacg
    18841 agtgagggaa aaaacatgaa taagttcaat gaaatttttt tagatttgga acagaaaatt
    18901 ttagcgaaag aatatcctcc gcattcgctt ttacccagtg agaaccaatt gatcaaaatt
    18961 tatggtgttt cacgagaaac aattagaaaa gcgctgaatc ttttgactac ggaaggttat
    19021 attcaaaaaa aacaaggaaa aggttcgatc gtactggatc ggaatcgctt cgactttcct
    19081 atttctggat tgactagcta taaagagcta caggagaccc aacgtattcc aagtgaaacg
    19141 atcatccata cgttagaaga aacagaagtg acaaaagcat tgaacgagat cactggctgg
    19201 gagatcggtg cgcctgtttg gcatttgata cgcgaaagaa aaatcaatgg cgaagtagtt
    19261 atattagaca ccgattatct attaaaggaa atcgttccta ttttaactcc agcacaagca
    19321 caaggatcga tctatgaata ttttgaaaat gagctttctt tgacgattgc ttacgcgcaa
    19381 aaggagatca cagtagaaga agtaacggaa atcatgaaaa cagcgatgga tcttcacgat
    19441 gacacttatg cagtagtagt cagaagcctc gttcatctgg aagatacccg ctgtttcgag
    19501 tattctgagt cggttcaccg attagataaa ttccgtttcg ttgagtttgc ccgacgccga
    19561 aaagtgtaaa tagttcaaaa gaaagcaaat tgttggcatc tgtccacttt ggcgctatga
    19621 taggatcaca aagggggcat ttttaatgga taaaccaata agaccagaaa aagaagaact
    19681 gaaagaaaag ttatcaccca tggcttatgc agtcacacaa gaaaatgcaa cagaacgacc
    19741 atttagcggg aaatatgatg atttttacga aaaggggatc tatgtagata tcgtcagcgg
    19801 tgaaccgtta ttcagctctg cagaaaaata tgatgcaggt tgtggctggc catcattcag
    19861 caaaccaata acgaaaagac aagtgcgcga aaaagccgat ttttctcatg gcatgcatcg
    19921 aatagaagtt cgaagcaaag aagcagattc tcatctcgga catgtgttta ccgatggacc
    19981 agtagatcaa ggtggtctac gttactgcat caattctgcc tcactgaaat tcattccata
    20041 tgaaaagatg gatgaattag gttatgggga attcaaatca cttgtagaat aatggaaata
    20101 cacaaagagc ggtacaatac ggggatttac tcgatcgtcc gctctttttt tattcaagcg
    20161 attgctattt gaaagaaacg ctccctagac tccttttttc acaaggtgaa atgtgctaca
    20221 ataaagggaa cgtatgtgtg caaaagaaag aggactagaa tatgtatgaa tatctgacag
    20281 gtgtggttac ctttatcaac ccctattaca tagtagtaga aaccaatggt atcggctacc
    20341 agatcgcact aggaaatcct taccgctaca gtagcaaact aaatcaaaaa ataaaaattt
    20401 atgtccatca ggtaatacgt gaagatgcgc atcttctgta tggatttgat tcgctagaag
    20461 aaaagcaact gtttttgcga ttagtaagtg tttcaggaat cggtccgaaa agtgcccttg
    20521 caatcatggc aagcgaagac cactccggtt tgattcaagc agtagaatcc agtgatgtca
    20581 cttatttgac gaaatttcca ggagtaggga aaaagacagc acaacaaatg atcttggatc
    20641 taaaaggaaa attcggtgag ctgagtatcg atacaccttt tagtctattt gatgaagcaa
    20701 acacaaaaga cgctacagct ctatctgaag caatggaagc tctctcggct cttggttaca
    20761 gtgatcgaga aatcaaacgt gtagaaaaac aattaaaaga agtagataat cagacaacgg
    20821 atgagtatct acgccaagca ctcaagctaa tgatgaaaaa ataaggagga gaccaaatga
    20881 cagatgaata cttgttatca ccagaatctg gcgaaaatga actgagtttg gaaaaatctc
    20941 ttcgaccaca gctgctttct caatatatcg gacagagaaa agtcaaacaa gaacttcaga
    21001 tttacatcga agctgccaga aatcgggaag aacctttaga tcatgttctg ttgtacgggc
    21061 ctcctggatt aggaaaaacg acgatggcca tggtcattgc taatgagatg accgtaaata
    21121 ttcgtacgac gagtggccct gctatcgaac gtccgggtga cttagtagca atcttgaatg
    21181 aactggaagc aggggatgtc ctgtttatcg atgagatcca tcgattacct agagtggtag
    21241 aagagatgct ttattcagct atggaagatt tctatgtaga tatcatggta ggacaagggc
    21301 caaccgcaca tccggttcat ttccctcttc ctccctttac tttgattggc gcgacgacac
    21361 gggcaggtat gctttctgca cctttacgag atcgatttgg catcatttct catatggaat
    21421 attatgacga acaggatctg caggagatcg ttttacgttc ttcggatatt tttcaaacag
    21481 agatcattga agaaggggca ttggaaattg ctcgccgatc acgaggaacg cctagaatcg
    21541 ctaatcgctt gctcaaacgg gttcgtgatt ttgcgcaagt ccaaggagac gggaaaatcg
    21601 atcgtcctat agcggatcag gcacttactt tgctacaggt cgatcaggct ggattggatt
    21661 atgttgacca gaaattgttg cgaacaatga tcgagttata tggcggcgga ccagtagggt
    21721 taagtacgct ttcagtaaac ataggagaag agcgtgagac ggtagaagat atgtatgaac
    21781 cgtatctgat ccaaaaagga tttttaaagc gtacacctag agggcgaatt gctactgctt
    21841 atgcctatga acattttggt tacgactatc atatatgatt tttgctttta gattggagga
    21901 aaatgaatga gtgatacaac acgtacgaaa caattgatca aaaacgtatt gattgatttg
    21961 tgccaaacgc aatcttttcg aaaaatcagc gtccaaaaaa tcgcaaacga agcaaaaatc
    22021 aatcgacaaa cattttatta tcattttaca gataaatacg atttgcttcg ttgggtctat
    22081 tgtgaggatg cattgcatta tttagatcca caatgtcttt cacttgataa ttgggaagag
    22141 caagcattga aaatgctgaa agcaattgct gagaagaaag aattttatgc aaatactgtg
    22201 ctttctgacc cagaaatttt acaaaaagag ttttctcaac tgatccaaaa atcatttatc
    22261 aagatttttg aacaaatgga tgaagaaaga caactttctg caagagacaa actattttat
    22321 gctcgttttt tttcttacgg ttgcagtggt gtattagtca attggatcac gaatgaatat
    22381 gaagaaacac cgatggaaat cgctatgcag cttttccgtt tagccaagga tacggaatta
    22441 tattcttatc gtctttatgc aaaagaagag ttggaagaac agtaatagaa aaattttttg
    22501 ttggaaaagc aataatcgct aaaaggagat ggggaaatga caaaaatttt atttgtttgt
    22561 ttgggaaata tttgccgttc accgatggca gaaggcttgc tgagaaaacg aatagcatta
    22621 gaaggaaggg aagcagaatt ttttgtggat tctgctgcta caagcacgta tgaagttgga
    22681 aagacaccgc accctggaac gaaaaaaata ttaaaccaag aaaaagtaga tatgacgaat
    22741 atggttgctc ggcaaatcac accccacgat tttgaaactt ttgattggat cattggcatg
    22801 gatcaagaaa atgtagaaga actgaaacga agagcaccac gctcagcgca ggggaaaatc
    22861 catttgtttt taagtagcat tcctggaaaa gaaaaagaaa atgttcccga cccatactat
    22921 accaataact ttgatgagac atatcacttg atcacagaag gcctgaatca ttggtggaat
    22981 atttggattg atgaaaataa aataacaaac aatttgtaaa gttaacgctt tctttttaag
    23041 gcgatgttag agcatttcct tgtttttttg taaaatgtta ggtaaaataa aattaattaa
    23101 gtaacacttt cttagctaaa ggaggagtgc attcgatgaa cgaagagaca gtatcgccaa
    23161 tgaagaagct tacgaagtta gttactcata cttcgtatca actttttgat tcttttgtaa
    23221 atgacactga agaacatgct aagaatgtac gattgcaagg aaagtttatt cttgagctgc
    23281 aaaaagctgc tactgaacat agtctagtta tcttacaagt aagagcaaat cgtttgagta
    23341 gcaagtatga aacgatctca ggctgggtgg cgacaaaaac ggtcgaaaaa gaccgagtga
    23401 tgatccgcct acaaaacgaa gagcagcaga tccggatggt tcctgtagaa caaattgaaa
    23461 aaataacaat cctttcacca gcaggggaac aggaacggtt atcaaaataa tttataccag
    23521 atagaaaagc acgtatgagc ttgtaccatc aatcattact aatcaaaaat aaccgaatgc
    23581 tttaagctga tatcagtaaa acggaacgtt ttaggatgag tatcacagca tgacagcatt
    23641 gggttgatta gttgaatcga ttggatggta caagtttttt tgataaaaaa aggtcctgct
    23701 agccggggag ctagcaggat aaggagttaa aaatgaaaaa gtgttgttag ggttgtttgt
    23761 tggtatgctt atataatagt cactagttgt gaatattttg tgacgaaagt tttcgaaagt
    23821 tgttaagttt ttgggagcgt caataatttt gtgtaaataa attgtcctcc tgcaaaataa
    23881 ttagttactc agtaaacatt gaaactaatg tatcggttac ctgttgaaaa cctttatggc
    23941 ttctgtttag aaatttttga ttgtatgtat caaaaatgct gactagaaag cgttctagtg
    24001 attcttcatt ttgaaactgc tc
//
LOCUS       DAZKLM010000038        23836 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3764
            SAMEA115706913-rid22582563.denovo.038, whole genome shotgun
            sequence.
ACCESSION   DAZKLM010000038 DAZKLM010000000
VERSION     DAZKLM010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706913
            Sequence Read Archive: ERR13196647
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23836)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23836)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287823.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287823.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,004
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,849
            CDSs (with protein)               :: 2,849
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 41 of 96
            Pseudo Genes (incomplete)         :: 52 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 15 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23836
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706913-rid22582563.denovo.038"
                     /isolate="VRE3764"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            385..1824
                     /locus_tag="ACG842_001386"
     CDS             385..1824
                     /locus_tag="ACG842_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFQ0247939.1"
                     /translation="MYSYQEDAKVQKNRWWILIAISMFTFMSTLDGSIVNIALPTISD
                     DMGIPMNQAEWVVSVYLMIVCSCLLLFGKIGDSRGKIKVFRIGTLIFVLGSLLCGFNH
                     SLGILLFARVVQAIGASMTMATNAGIITEVFPIHERGRALGAIGAFVSLGSIAGPGLG
                     GLILSQFSWAYIFWINVPVGIVTMLIAEKFLPKDITMSGHTIDGGGFAAIAATIISFF
                     GAIFLGQESGFFHPVPLVLFVISVIAFVSFLKVEKKAASPLISFGIFKNKIFTMSLIT
                     ALLIFSSNFFVNVVIPFYLQNARGLSPSYAGLLMMVFPLMMVVGAPLSGYLTDRVGPE
                     ILVLVGLGLLSVTQVMYMFLSVDSPLWLYIVATAIMGAGNALFQSPNNTMVMSSVTIE
                     NLGLAGSMNSFARNLGMVSGISLSTTVLYQAMSAKMGQQVTNYIANRPDVFLYGMKIT
                     FLGSFVLCFVAFFLTIWRIKKQGGIQKNV"
     gene            2119..2337
                     /locus_tag="ACG842_001387"
                     /pseudo
     CDS             2119..2337
                     /locus_tag="ACG842_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
     gene            2586..2801
                     /locus_tag="ACG842_001388"
     CDS             2586..2801
                     /locus_tag="ACG842_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0247940.1"
                     /translation="MNAGIADMNLIKKTLNDFTSNSISKGTGINLSTIKKLKSGERSV
                     EKLNLLDAIKITEFAMKNGKAEIEIWR"
     gene            complement(3018..3274)
                     /locus_tag="ACG842_001389"
                     /pseudo
     CDS             complement(3018..3274)
                     /locus_tag="ACG842_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(3461..4372)
                     /locus_tag="ACG842_001390"
     CDS             complement(3461..4372)
                     /locus_tag="ACG842_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFQ0247941.1"
                     /translation="MKIKIERNQKEKSPKKKKERVVSVGKHRKMVLALWLLLSCSLAF
                     GVYKNFTAIDQHTTHEKVVIKEKVVNTSGVESFTEDFAKEYFSWKNNKEAIEKRMSNL
                     EQYLTENGLALIQDMVRADIPTSSEVQSVKILDVEKHSEEFVVSFLVEQHITEGKKTQ
                     SISSAYRVTIFEDENRNHLVTSLPTMIGKPDKAKYEAKQVENDSEIDAKTTEEITDFL
                     ETFFKLYPSASEKELEYYVENGAMRPINIDLKFSELENPVYLQNGKNFLVSTKIKYLD
                     NVSKTTSYFQYKLVLQKNGNWKIVDVK"
     gene            complement(4391..5395)
                     /locus_tag="ACG842_001391"
     CDS             complement(4391..5395)
                     /locus_tag="ACG842_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HFQ0247942.1"
                     /translation="MNLKKVGVISLTFLTISFMGILMCVGLLFGEDSEGNGSSGTSPG
                     GTSVSEEVLKHRAMVEKYAKESDITEYIPILLAIIQVESGGTMEDVMQSSESAGLPPN
                     SLSTEASIKQGCLYFASLVKRSKELGCDQDSIIQAYNYGGGYLDHVGKNGKKHSFALA
                     ESFSKEKSGGQKVDYPNPIAIKENGGWRYNYGNMFYCLLVKQYLTTTQFDDKIVQEIF
                     DEAFKYEGTAYVFGGSSPETGFDCSGLTQWCYAKVDLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLVFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTENYWRQHLIGAGRYK
                     "
     gene            complement(5392..7515)
                     /locus_tag="ACG842_001392"
     CDS             complement(5392..7515)
                     /locus_tag="ACG842_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CD3337/EF1877 family mobilome membrane protein"
                     /protein_id="HFQ0247943.1"
                     /translation="MKKKLFRIVGIVFISILTLIVLLSLVGTVAEATGLVDDTVEAGN
                     LYSKYSLNNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAESIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRAVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALTLGTEIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEIGEDRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKKAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMSMFNLQSNDSQSMGRRVMRKPQMLMNRKLR
                     QLNRNVGRALAFGGGAAVGNKLAKEQSKSKFKPSGSSLRKNSRLPNDHEILSDLTKEN
                     PVSNNKKQSRMNLAGRKIGKVLDTQALVKDKTKQVKDQVRNTPTNLKYNLHKGIEKSK
                     KAPEEFKRGLFQEKADRVDLREKQRQRRNAKMDEKRKILSEATNRQGKRRANIPIDKA
                     AQPQKDMMLKKEAPKRIVRANLNPEIKRKLTSQEIISKGKSQSASKKNSFQQVKQRST
                     LPKQTNQKVQKRMNHRPKSRSGEKK"
     gene            complement(7520..9967)
                     /locus_tag="ACG842_001393"
     CDS             complement(7520..9967)
                     /locus_tag="ACG842_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFQ0247944.1"
                     /translation="MYPIKYIENNLVFNQEGECFAYYELIPYNYSFLSPEQKYQVHDN
                     FRQLIAQNREGKIHALQIATESSIRATQERSKKEITGRLTEVAKQRIDLQTEALVSMI
                     GDSQIDYRFFIGFKLIATDEEVNLKNLKKSFFSGFQEFVYGVNHHLMGDFVSLSNEEI
                     RRYTKLEKLMESKLARRFKVRRVTPSDLMYLIEHIYGEKGTPFEEYEFQLPKKKLKSE
                     TLVKRYDLLRPSRCLIEEKPRYLCMEHENHESYVAYLTINTIVGEMEFPSSELFYYQQ
                     QQFTFPIDTSMNVEIVTNKKALATVRNKKKELKDLDNHAYQSDNETNSNVLDALDSVD
                     ELETTLDQSKESMYKLSYVVRVSAESVDELKRRCDEVLDFYDDTNVKLVRPFGDMMGL
                     HEEFLPSSKRYMNDYIQYVTSDFLAGLGFGATQMLGELEGIYFGYNVDTGRNVYLKPA
                     LASQGVKDSVTNALAAAFLGSLGGGKSFSNNLLVYYAVLFGGQAVIVDPKGERGGWKE
                     TLPEIADEINILNLTSKPKNQGLLDPYVIMEKKKDSESLAIDILTFLTGISSRDGEKF
                     PVLRKAIRTVTQSNQRGLLQVINELRKEGSPVAENIADHIESFTDYDFAHLLFSDGSI
                     TQSISLDRQLNIIQVADLVLPDAETTFEEYTTMELLSVSMLIVISTFALDFIHSNREI
                     FKIVDLDEAWSFLQVAQGKTLSNKLVRAGRAMNAGVYFVTQNSDDLLDERLKNNIGLK
                     FAFRSTDIHEIKKTLEFFGLDREDESNQKRLRDLENGQCLMQDLYGRVGVVQVHPVFE
                     ELFHAFDTRPPVQERME"
     gene            complement(9954..10343)
                     /locus_tag="ACG842_001394"
     CDS             complement(9954..10343)
                     /locus_tag="ACG842_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFQ0247945.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLLFV
                     IVFAHVPPLSMIEGAFLKYLGIPVAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPITLVRSVNYVPD"
     gene            complement(10403..10906)
                     /locus_tag="ACG842_001395"
     CDS             complement(10403..10906)
                     /locus_tag="ACG842_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HFQ0247946.1"
                     /translation="MEQMRVYIANLGKYNEGELVGAWFTPPVDFDEVKERIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCGLAEELEGTPVGEVASEIQHAFFNSFEEMVEH
                     VDDIIYYPDCDDMSDVAYFLIVEAGDFGEVPEHLKTYIDFDACGRDLEIGGNYLVTSR
                     GVFEYPY"
     gene            complement(10919..11143)
                     /locus_tag="ACG842_001396"
     CDS             complement(10919..11143)
                     /locus_tag="ACG842_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0247947.1"
                     /translation="MSGTYLSIYQWFLTNAQSLVLLAIVVIGLFLGFKREFSKLIGFL
                     VIALIAVGLVFNASGVKDVLLSLFNRIVGA"
     gene            complement(11247..13157)
                     /gene="ltrA"
                     /locus_tag="ACG842_001397"
     CDS             complement(11247..13157)
                     /gene="ltrA"
                     /locus_tag="ACG842_001397"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HFQ0247948.1"
                     /translation="MPKNKNETLCVEDLRHAEYYEMQNTFDDLYAKSKNGDIFTHLMD
                     IILSRENILLAYRNIKANAGSKTAGTDGTIIKDIGKLPAETVVKKVRYIVAGSPHGYR
                     PKPVRRKEIPKPNGKTRPLGIPCMWDRLIQQCIKQVLEPICEAKFSENSYGFRPNRSV
                     ENAIKATYNRLQISQLHYVIEFDIKGFFDNVNHSKLIKQIWAMGIRDKHLIFILKRIL
                     KAPIKMTNGTITYPEKGTPQGGIISPLLANIVLNELDHWVESQWQENPVTKNYVVHIN
                     KSGSPCKSNAYKEMKKTKLKEMYMVRYADDFRVFCRYKESAEKAKIAITQWIEQRLKL
                     EVSQEKTRIVNVRKRYSDFLGFKIKMIPRRKKLVVKSHISDKQFKNQKDKLVSQAKKI
                     ASPSKGKTELEETRLYNSMVLGMQNYYQIATNVNLDCMKLNRAVMAIFTNRLGTNKRG
                     RLRKTGRQLTTKEATRYGKSKMLRYVAGEGEPIFPVGYIQHRKPMAKVYLANCYTLEG
                     RKFIHTNLSVDKLLMYQLMKLSLENRTIEYADNRISLFSAQHGKCAITLEEFQQANEI
                     HCHHIVPTGAGGNDDYKNLILVKEAVHRLIHAKTEETIQKYIALLKLNKVQLIRLNKY
                     RVLAGNQELNLI"
     gene            complement(13903..14040)
                     /locus_tag="ACG842_001398"
     CDS             complement(13903..14040)
                     /locus_tag="ACG842_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HFQ0247949.1"
                     /translation="MKRGIVMGIVKDIFLVFGGSMLGVTVMCLMNASAAADRAMEKKG
                     K"
     gene            complement(14037..15221)
                     /gene="mobT"
                     /locus_tag="ACG842_001399"
     CDS             complement(14037..15221)
                     /gene="mobT"
                     /locus_tag="ACG842_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MobT family relaxase"
                     /protein_id="HFQ0247950.1"
                     /translation="MAQRNLDYRLLKDRRNEYGVSQNKLATACGLSRPYLNQIENGGV
                     TASIKTMRKIFDQLESFNPDLPLTLLFDYVRIRFPTTDARKIIQEILHLKFEYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCL
                     KAGGVMKRLDLAINDRVGLLDIPELTKKCQKEECISLFRTFKSYRSGELLKADEKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAFHAIQDLL
                     KGRSIESTTFSIINRYLRFADKVEGKRRTNWPLNEQWGRFIGRNRKEIQLTSEPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNRTTYIQDMVQNARLSDRHKKILEQQSMAIENL
                     II"
     gene            complement(15480..15770)
                     /locus_tag="ACG842_001400"
     CDS             complement(15480..15770)
                     /locus_tag="ACG842_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0247951.1"
                     /translation="MVYDELIGEIYWVIGKIQSDPELEEQLRRLKFDIRKNGVKVAGD
                     PYLMNEETDARIEINQVIAEFERIADQAKEPDIRQYLLEMKAELEVNGITDE"
     gene            complement(15760..16902)
                     /gene="dcm"
                     /locus_tag="ACG842_001401"
                     /pseudo
     CDS             complement(15760..16902)
                     /gene="dcm"
                     /locus_tag="ACG842_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613853.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
     gene            complement(16978..17142)
                     /locus_tag="ACG842_001402"
     CDS             complement(16978..17142)
                     /locus_tag="ACG842_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0247952.1"
                     /translation="MTEKEQVTKIVKKYNKSIADLSENATAKEFKTVIKYVADQANEK
                     QRKLVGLNKK"
     gene            complement(17135..17809)
                     /locus_tag="ACG842_001403"
     CDS             complement(17135..17809)
                     /locus_tag="ACG842_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HFQ0247953.1"
                     /translation="MESNDSYLEYAKANKDKFISYIIKGKSPEVGRDAVFMAGSPGAG
                     KSEVAIGLAENYDNHVIIDADYFRTQFPDYNGKNSSLFQKASSWLVEQAFRYVLDHGY
                     SFILDATFAILSAEKNISRALKKHFRVTIFYVYQDPKIAWKFTQEREIAEGRHVPKKA
                     FINAFFKSRENIAKVKSKHPDVLLHIIIKDYTNNISEVHYATDNLELFLPLGYSRKDL
                     EVQLHD"
     gene            complement(17887..19227)
                     /locus_tag="ACG842_001404"
     CDS             complement(17887..19227)
                     /locus_tag="ACG842_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsK/SpoIIIE domain-containing protein"
                     /protein_id="HFQ0247954.1"
                     /translation="MYKGHRIRAGDQHLVYHFVLGWLLALFIGWMSVFYFQEFRQFDI
                     SKLSLSTIEIVWSIKDLVCLLGSLAFSGAMILLYIHFFLDHWRSLWHRQKLARMILEN
                     HWYEVKQTQSEGFFKDLNSSRTRETISYFPKICYRMKDGLLSIRVQISLGKYQDQLLK
                     LEKKLESGLYCELVEKELKDSYVEYTLLYDMIANRIGIDEVVAENGTLRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIEALLKSDAELFILDPKNADLADLGTVMPHVYSQK
                     EEISACVEDFYERMMARSKAMKEMSNYKTGENYAYLGLPPNFLIFDEYVAYMEMLTTK
                     ESAVILNKLKQIVMLGRQSGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSELGYS
                     MMFGEVEKNFFMKHIKGRGYVDTGGSVISEFYTPLVPKGYDFLDSISKLEKMND"
     gene            complement(19310..19645)
                     /locus_tag="ACG842_001405"
     CDS             complement(19310..19645)
                     /locus_tag="ACG842_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein J"
                     /protein_id="HFQ0247955.1"
                     /translation="MEINVEHILDCLDQYGKGELTEEQLVKALTSDEKMFLIIHQGLL
                     GENSDSEEDSDVLDWLGEEESFFIVVEVNENLCRQAESILEEIGVEMPDAIEGFLNQL
                     VETKQLPSS"
     gene            complement(19655..20029)
                     /locus_tag="ACG842_001406"
     CDS             complement(19655..20029)
                     /locus_tag="ACG842_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HFQ0247956.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTIGNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIVDTVANYVFREGTTVNWFIKA
                     DDLVLKRQPNQGNPTNQNERKK"
     gene            complement(20042..20356)
                     /locus_tag="ACG842_001407"
     CDS             complement(20042..20356)
                     /locus_tag="ACG842_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HFQ0247957.1"
                     /translation="MELKFVVPDMAETFGKLSYAGEGEIITEGYGRNTTVIGRSYHLY
                     SSKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLVAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            complement(20469..23696)
                     /gene="fss3"
                     /locus_tag="ACG842_001408"
     CDS             complement(20469..23696)
                     /gene="fss3"
                     /locus_tag="ACG842_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
                     /protein_id="HFQ0247958.1"
                     /translation="MYKKQKKKLCVLTILSLAVQAIVGAIAPTITFADEITHPQTVTV
                     ELDLAHQYAVEGTFSDGRPMSEVTVPHYAVYNGFKQDIFCIEPGVPIYNEFTPGYEKN
                     PLPDMSDKAKLVSVLWKKVGTDADTHIVAQKMIWQEVNGYTLHSIKRSDGSAVNIAAI
                     EAKINQAIDDYQKKPTFHNSTAKTVLGQSTTVTDTNNLNLSEFDEVVENAANIDYRVN
                     GNQLIITPNANSKESGVLTLKKSAGTGTPVAYKKAGQQTLMAGAIDKPNTYTIKIDVE
                     TEGSLKIKKVDKESGDVVPGTVFHLDFGKTLPAKDVTTDKEGIATLDEIPHGTKVTIT
                     EKSVPAPYTIDTTPMTTTIKAGETIYVTSKNAREKGQIILDKSGVETGSDLWNDNYSL
                     AGNTFAIRKDSPTGEIVQEMTTDENGHAETPKEIANALELGTYYVTETKASHGFVNTF
                     KPVKVELKYANQTVAPVTSNVKGQNQEVTGETTLTKEDKDTGDKAQGKAVFEGAEYTL
                     FTAKDGKAVKWSEAFKPELAKGTKGSDETVTLTLDEKNQVAVKHLAINEYYWQETKAP
                     EGYSLDETKYPVSIKKVDDSEKNAVITRNVTAKEQVIRFGFDFFKFAGSAAGTTETGF
                     NDLTFKVSPLEGTNEITGAEDEATTAYNEQLGFDGYGKFENLPYGDYLLEEVEAPEGF
                     QKITPLEIRSTFKENKEDFVKSEYVFTITEQDQKQPIKTVTVPYEKLTNKAFSVSLNR
                     LMLYDLPEEEDSLTSLATWKDGNKELTSLDSTELVDKLSYNLHEIKEDWYVVAQAIDV
                     DATKAAQEKDEKAKPVVIAETSATLANKEKTGTWKIQHKLTAEQVLNKTIVLFNYVYE
                     NKEAFEAGDKPVAKDVSLNNQAQTVSCTVEHHVSIQTKAHLENGSQTFTHGDVVDMFD
                     DVSITHDVLDGSKEAFETILYALLPDGTNKEIWKSGKIDYEVNDKEFTKTVLAKKVDT
                     GKYPEGTKFTFAEINYDKDGNINGKHNEDLKEKSQTLTPKEVPTILSTPKQPETPTVP
                     SGSQESSPTVKTFPQTGEKNSNVLLFIGFTLIFATAGYYFWNRRN"
BASE COUNT         6670 a         5145 c         4111 g         7910 t
ORIGIN      
        1 tttagtataa tcctatggaa ttgaatatca tagcaatgta tttgaaaagc aaattggaaa
       61 acaaataggt gcaacaccat aaatatgaaa aaattgggaa aaagtagaat ggaaatttgc
      121 ttacgtctca cgagatagct cacactatta atctgaagaa caatgaagtc gacttttaga
      181 aattgctaga agaaagtaaa gcgaattatc actagttact atagagtaag ttcaaataaa
      241 gcatctccaa cttacatggt tacatgagga tatcaaatcg aaattactca agcttcctat
      301 ggctagagtc gcgagaagtt gttgggcagt agacataggt tatattgttc ttagctacac
      361 gatcattaga aaggaaatcc tattatgtat agttatcaag aagatgcaaa agtccaaaaa
      421 aatcgctggt ggattttgat tgctatatct atgtttacct tcatgtccac attggatggc
      481 agtattgtca atattgcact acctaccatc tcagatgata tgggcatacc aatgaatcaa
      541 gcggaatggg tagtgtccgt ttatctgatg attgtctgtt cttgtttgtt gctgtttggt
      601 aaaattggcg atagccgtgg gaaaatcaaa gtatttcgga ttggtacgct gatctttgtc
      661 ttgggatcgc tgttgtgtgg gttcaatcat tcgctaggta ttttattgtt cgcacgggtg
      721 gtacaagcca ttggtgctag tatgactatg gctacaaatg ccggaatcat cacggaagtc
      781 tttcccatac atgaacgtgg tcgggcattg ggagcaatag gtgcgtttgt ttcattaggc
      841 tcgattgctg gaccaggtct tggcggcttg attttgtccc aattttcttg ggcgtacatt
      901 ttttggatca atgtaccggt gggaatcgta acgatgttga ttgctgaaaa attcttacca
      961 aaagatatca ccatgagcgg ccatacaatc gatggagggg gctttgctgc cattgcggct
     1021 acgattatca gcttttttgg agcaatcttt ttggggcaag aaagtggctt tttccatcca
     1081 gtcccccttg tactgttcgt aatttcagtg attgcctttg tcagcttttt aaaagttgaa
     1141 aagaaagcag cctctccatt gatttctttc ggtattttta agaacaagat ttttaccatg
     1201 agcttgatta cagccttgtt gattttttct tccaacttct ttgtcaatgt agtgattcct
     1261 ttttatctgc aaaatgctcg cgggttgagt cctagctatg ctgggctgtt gatgatggtc
     1321 tttcctttga tgatggtcgt cggggcgcct ttaagcggct acctgaccga ccgggttggc
     1381 ccggagattt tggtattagt aggcttgggc ttattgtcgg taacccaggt catgtatatg
     1441 tttttgtcag tggactctcc tttgtggcta tacattgtcg ctactgccat catgggtgcc
     1501 ggcaatgcct tgttccagtc ccccaataat acgatggtta tgagctcggt cactatcgaa
     1561 aacttgggac tggcaggctc gatgaattcc tttgcccgaa atttggggat ggtcagcggg
     1621 atttcgcttt ctacgacggt cttatatcaa gcgatgtctg ctaagatggg ccagcaagta
     1681 actaactata tcgcaaatcg gccggatgtc tttttatatg gaatgaagat taccttttta
     1741 ggcagctttg ttttgtgttt tgttgccttt ttcctgacta tctggcgtat caaaaaacaa
     1801 ggaggtattc agaaaaatgt ctgaaaaaac cactgttgaa gaatttttgg aggcctgcgt
     1861 atgggtgagt gggatttcca tggatgatgg aagatgaagt gaacttctaa aagccgaaaa
     1921 actcaagtat tatcttcaaa atactagtgc tcaatgagat gcttgatttg atcacaagaa
     1981 gactgcgaaa gccgctggca accgcaagct aggtatccca ccgatggact acgttttaga
     2041 ggctactggt tatgaatacg gagccaacgc tctcatctgc ataagaatac atcacccaga
     2101 ttatgtaaag attttcgata cttctgtcaa aaaatcatta agatagataa atgatttccg
     2161 tctagtcaaa ttggttcaga atgtagacac acagatggta agaagtcact cacaattcga
     2221 gaatggactt gtcctatttg ccgtatacat cataatcgaa acgtcaacga tagtatcaat
     2281 attttgacca agggctatga atacaagcat tgccttagaa aacatcaaga accttaggaa
     2341 caacggggat agcttggtta ataagagata ccgctgttta ggcttgcaag tatactcttt
     2401 tatcaagaat tccgtggctt tagtcatgag aggcttaata ctatgaaaat tttctttagc
     2461 atttttgatt taggttcctt ttctaattat aataagatga ccactacagt aacctactga
     2521 aatatataaa cagccactgt agtaccattt tcaaagtagt ctatgagagg agaatcttca
     2581 ttcttatgaa cgctggaatt gcggatatga atttaataaa aaaaaccctt aatgatttta
     2641 cttccaattc aatatcaaaa ggtactggta tcaatttaag cacaataaaa aagctgaaaa
     2701 gtggtgaaag atcagtggaa aagctgaatc ttttagatgc tataaaaata acggaatttg
     2761 ctatgaaaaa tggaaaagct gagattgaaa tatggagata attatcgtct agtgaattac
     2821 ttgccactta gctgaattta acgatccttg gtgcttgaag ggggagattc ttgggaatag
     2881 agcatacttg caagcctatg tctttactaa caacgatatc tcttattaac caagctatcc
     2941 ccgtagttct aaggctttta cttttatcta agcaataatc gtattcaaag gaaatttttg
     3001 acccttagaa agtctcattt tatcagacgt gagcttaggc attgctgaat tttgttaatt
     3061 cggttttagg ttgtgctttg agcataatat gaaagtattc tttatcacga tttcattcat
     3121 ctaaggtaat gtgatgtgat tctgaaattc tttcaaaggc agtttttgca taagctgaca
     3181 ttacatcatc aatgacatgt ttacaatgtt tcactactaa aataaggggg taaccaagaa
     3241 gaaaaactga atgattattt gtatctaatt ccatggtata tcaaccattt cctatataga
     3301 ctgattatga catggattaa aataaaaagg cactatgtct taattgtcgg ttttcgagtt
     3361 accgaatggc attcatcacc cacctataga ggatgggcga cttctgcctg aaataagtta
     3421 cactacttat aacttctaat aaaattagat tgagtcatta ttatttaaca tcaacgattt
     3481 tccagtttcc atttttttgt aatactaatt tgtactgaaa gtagctagtg gtttttgata
     3541 cattatcaag gtattttatc tttgtactca ctagaaaatt ttttccgttt tgtagataaa
     3601 ctggattctc taactccgaa aactttaagt caatattgat tggtcgcatc gcaccatttt
     3661 caacataata ctccaattct ttttcagaag ctgatggata aagtttaaaa aatgtttcta
     3721 aaaaatcggt gatttcttcc gttgttttgg catcaatttc tgaatcgttt tctacttgtt
     3781 ttgcttcata ttttgccttg tctggctttc caatcattgt tggcaaacta gttactaaat
     3841 gattcctgtt ttcatcttca aagattgtca ctcgatatgc agaagaaatt gattgcgtct
     3901 tttttccctc tgtaatatgt tgctccacta aaaatgaaac gacaaattct tccgagtgtt
     3961 tttcaacatc tagaattttt acagattgaa cttcggaact cgtgggaata tctgctcgaa
     4021 ccatatcttg gattaaagcg agaccgtttt ctgtaaggta ctgttcgaga ttactcattc
     4081 tcttttcaat tgcttctttg ttatttttcc atgaaaaata ttctttcgca aaatcctctg
     4141 taaaactttc aacgccagaa gtattcacta ccttctcttt tataaccact ttttcatgag
     4201 ttgtatgttg atcaatggcg gtgaaatttt tataaacgcc aaaagcaaga ctacaagaca
     4261 ataaaagcca aagggcaagc accatttttc gatgcttacc cacggataca actcgttctt
     4321 ttttcttttt tgggctcttt tccttttgat tccgttcaat ttttatcttc attctttttc
     4381 ctcttttctc ttatttgtat cggccagctc cgataagatg ctgccgccaa tagttttccg
     4441 ttagattcgt ccaccccact ggatctcctg catgaaacat gcgattctcc cctgcgtaaa
     4501 tgcccacatg ggtcacataa gtaccggcat cataggttga atgaaagaaa actaaatcac
     4561 ctggtttggc atcttttaga tcaatatgag tcatggcatc atattgatcc tgagccaccc
     4621 gaggaagttt caggtcaact ttggcataac accattgggt caagccacta caatcaaacc
     4681 ccgtttctgg acttgagcca ccaaacacat aggccgttcc ttcatactta aatgcctcat
     4741 caaaaatttc ttggactatt ttgtcatcaa actgagttgt agttaggtat tgcttcacta
     4801 ataaacagta aaacatattg ccataattat accgccaccc accattttcc ttaatcgcga
     4861 ttgggttggg gtaatcgact ttttgaccac ctgatttttc ctttgaaaaa gattcagcta
     4921 aagcaaaact atgcttcttc ccgtttttcc cgacatgatc tagatacccg ccaccatagt
     4981 tataggcctg aataatacta tcttgatcac accctagttc tttggaacgc ttaaccaaag
     5041 acgcaaaata gagacagcct tgtttgatgg atgcctcggt acttaatgaa ttaggtggta
     5101 atccggctga ctcagaactt tgcataacgt cttccattgt gccaccagat tccacttgaa
     5161 taatcgccaa aagaattgga atatactctg ttatatccga ttctttggca tacttttcca
     5221 ccattgcacg gtgtttcaat acttcttctg agacactagt tccgccagga gaagtgccag
     5281 aactaccatt tccctcactg tcttcaccaa acaacaaacc cacacacatc aaaataccca
     5341 taaaagagat ggtcagaaaa gttaatgaaa taacacccac tttcttcaag ttcatttttt
     5401 ctcacctgac cttgattttg gacgatggtt cattcttttt tgtacttttt gattggtctg
     5461 ctttggcaga gtggacctct gtttcacttg ttggaaagaa tttttcttac ttgctgattg
     5521 gcttttccct tttgaaatga tttcttgact agttagtttt cgtttgattt cgggattcag
     5581 atttgctctc acaatccgct ttggtgcttc ttttttcagc atcatatctt tctgaggttg
     5641 agctgcttta tctattggaa tattcgccct tctttttccc tgacgatttg ttgcttcact
     5701 cagtatcttg cgtttttcgt ccattttcgc atttcttcgt tgtcgttgtt tttctcgcaa
     5761 atccactcga tccgcttttt cttggaacag tccgcgcttg aattcttctg gtgctttttt
     5821 cgacttttcg attcctttat ggagattgta tttcaaattc gttggcgtat tccttacctg
     5881 atcctttact tgtttagtct tatctttcac caaagcttgg gtatccaaaa ctttgccgat
     5941 ttttctccca gccaaattca ttcgggattg tttcttatta tttgaaactg gattctcctt
     6001 agttaagtca gatagtattt cgtgatcgtt cggtaatcgt gaattctttc gtagagaaga
     6061 accggatggc ttaaatttgg attttgattg ttcctttgcc aacttatttc cgactgctgc
     6121 accaccacca aaagccaaag ctcgtcccac atttcgattg agttgccgca attttcgatt
     6181 cattaacatt tgcggttttc gcatgacgcg tcttcccatt gactggctat cattactctg
     6241 gaggttgaac atactcataa tgtcgcccaa tttgaaatag ataccggcaa aggtcacaat
     6301 ttgcagaaaa gcaaccatga aaaatggata ggttgccgaa atattgaaaa acatcgtgga
     6361 gatgctaaat gcagtcgtaa tcaccaaagt aacacccgct cgcatcataa tcgtattgaa
     6421 taaacgaata atcgccttct ttcctaaact ttcataagtc ggcaacatgg acaataagaa
     6481 actaattggc agaaacatcg caaagataat gaagagaatt tgcgagaaca acataatccc
     6541 tgttagaaga aaaacaaaga aggaaatgcc aatattgaac aataccaaaa agaccacggt
     6601 ccctaaacgg ttcatggtct tggtaatact gagattcgca ttgtcattgt cttcaatctc
     6661 tgccttgacc gcttcttccc gatctttgcc tttgttttca tctggactca cagatagaat
     6721 cttgttgacc cggtcttcac caatttcttc tatgttggaa tcatcaaatt gcagtagtaa
     6781 ccaaggttgt tggacttgaa tcgaaaacaa actatcccga atcaaatcca cactatcttt
     6841 tccttggctt tctgaattgg gtacaacgat ctcggttcca agggtaaggg ccgcttcact
     6901 aacatctgag ctaaagtcat tgatttttgt gatataggta ggcgcataag caatgaacga
     6961 acccgatagt agaaaaatca tcacaaaatt aatcacggct cgaactgctt tcgtggtttc
     7021 tcgttttaat aatccggtat acgccacata cacccccaga gctaaaatca tcaataatag
     7081 aaacccaaaa taaaatccgg aggtctggag tccattttca gtaataccag ccaatgtttg
     7141 gatattcttt ccaatacttt cagccgtatc cgaaatgaaa tctaatttgt aagcctcttg
     7201 aaccacataa cccgtggcgt tcgacaaata caaactcacc gtccaaatga aattagtaat
     7261 ggcataaaga ccatacatca cgcttttccc taagccatca ccccaattcc aaggcaacca
     7321 atcccaagag ctatccacaa agaaatctag ttgatagttg ttcagtgaat atttggaata
     7381 tagatttcct gcttcaactg tatcatcaac tagccctgtc gcctcggcta ccgtaccaac
     7441 taaactcaat agaactatca gagtgagtat gctaataaaa acgattccca caatccgaaa
     7501 aagtttcttt ttcatcccct cactccattc tctcttgaac aggtggacgg gtatcaaagg
     7561 catggaagag ttcttcaaag actggatgta cctgaaccac gcctacccga ccatacaaat
     7621 cctgcatcaa gcattggcca ttttccaaat ctcgtaggcg tttttgattg ctttcatctt
     7681 ctcgatccaa gccaaagaat tccaaggttt tcttgatttc atgaatatcg gtactgcgaa
     7741 acgcaaactt caacccgata ttattcttca agcgttcatc cagtaaatca tcactatttt
     7801 gcgtgacaaa gtaaaccccg gcattcatag cccgcccagc acgaacgagc ttattcgata
     7861 atgttttccc ttgcgctact tgaagaaaac tccacgcctc atctaagtct acaattttga
     7921 agatttctcg attactatgg ataaagtcca aggcaaaagt ggaaatgacg attagcatgg
     7981 aaacactaag caattccatg gtggtatatt cttcaaaagt ggtttctgca tctggtaaaa
     8041 ccaaatccgc tacttgaatg atatttaact gcctatctaa actaatcgac tgggtaatcg
     8101 aaccatctga gaaaagcaaa tgggcaaaat cataatcggt aaaggactcg atgtgatccg
     8161 cgatattttc cgcaactggt gagccttctt ttcgaagctc attaattact tgtaataatc
     8221 cccgttgatt gctttgggta accgtccgaa tggccttacg caacactgga aacttctccc
     8281 catctcgact agaaattcct gttaaaaagg tcagaatatc aattgctaga ctttcagaat
     8341 ccttcttttt ctccataatc acataaggat caagtaatcc ttgatttttt ggcttactag
     8401 ttaagttgag aatattgatt tcatccgcaa tttctggcaa ggtttctttc cagccacccc
     8461 gttccccttt tggatcgaca atcacagctt gtccaccaaa aaggacggcg taataaacta
     8521 agagattgtt actaaaggac tttccgccac cgagtgaacc gaggaatgcc gctgctagcg
     8581 cattggtaac tgaatccttg accccttgtg aggccaatgc cggtttcagg taaacattgc
     8641 gccccgtatc tacgttataa ccaaagtaaa tgccttccaa ctcccctaac atctgagtgg
     8701 cgccaaaacc aagtccggcg agaaaatcag acgtaacata ctggatataa tcattcatgt
     8761 atcgcttact agaaggtaag aattcttcat gtagccccat catatcgcca aaaggccgga
     8821 ctagttttac attggtatca tcataaaaat ctagcacttc atcacaccgg cgctttagtt
     8881 catctactga ttcggcactt actcgaacca catagctcag tttgtacatg gattctttgg
     8941 actgatccaa ggtagtttcc aattcatcta cggaatccaa tgcatctaag acattggaat
     9001 ttgtttcatt gtcagattga taggcatggt tgtccaagtc ttttaattcc tttttcttat
     9061 tgcgaacggt agcaagtgcc tttttgttcg tcacaatctc cacattcata ctagtgtcaa
     9121 ttgggaatgt aaattgttgt tgctggtaat agaaaagttc tgaggaggga aactccattt
     9181 cccccacaat tgtattaatt gttaagtacg ctacatatga ttcatgattc tcatgttcca
     9241 tgcataagta ccggggcttt tcttcaatca aacaacgact gggacgtaat aagtcgtaac
     9301 gtttcaccaa agtttccgac ttcagctttt tctttggtag ctgaaattca tattcttcaa
     9361 atggtgtacc cttttcgcca tagatatgtt caatcaaata catcagatca ctcggtgtga
     9421 ctcgtctgac tttaaatctt cgagccaatt tactttccat tagtttttca agcttggtat
     9481 atcggcgaat ttcttcattg gataaggaaa caaaatcgcc catcaaatga tgattcactc
     9541 catagacaaa ttcttgaaag ccggagaaga aagatttctt gagattcttt agattgacct
     9601 cttcatctgt cgcaatcagt ttgaagccaa taaaaaagcg ataatcaatc tgactatcgc
     9661 caatcatgga tactaatgcc tctgtctgta aatcaattcg ttgtttcgct acttctgtta
     9721 atcgcccggt aatttccttt tttgatcgtt cttgcgtggc acgaatacta ctttctgtgg
     9781 caatttgtaa ggcatgaatc ttgccttcac gattttgcgc aatcaattga cgaaagttat
     9841 cgtggacttg atatttctgt tcaggtgaca aaaaggagta attgtaaggt attaactcat
     9901 agtaggcaaa acattctccc tcttgattaa agacaagatt attttcaata tacttaatcg
     9961 ggtacatagt tcacactcct taccagagta atgggttcgt cccatcgttt cttctgttct
    10021 ttgactttct ttcctgcgaa agtgacctta aatcgtaagt gatagctaat aaaagatcgt
    10081 aaaaatccca atggtttctt accgtcaaac gttttttgcg acatgaacca agtaactgcc
    10141 accggtatac ctaagtattt taaaaaggcc ccctctatca tagatagtgg aggcacatgg
    10201 gcaaatacga tgacaaacag caaggataaa acaaaccaag ccatctggtt aaaagtcaca
    10261 ggaaaaggca gttgaaagtc attaattgca taaatgacct tttcaactgc ccaaatactg
    10321 gtgtagcttt ttattttctt catcaaatca ttcctttaaa aagaaggata gtttagaaat
    10381 caactaccct tcaataattt tgttaatatg gatactcaaa aacacctcga ctcgttacca
    10441 aataattgcc tccaatctcc aagtctctac cacacgcatc aaaatcaata tacgtcttca
    10501 agtgttctgg tacttctcca aaatcaccag cttctacaat caagaaatat gctacatcac
    10561 tcatatcatc gcaatctgga taatagataa tatcatccac atgctctacc atctcctcaa
    10621 aagaattgaa gaaagcatgt tgaatctcag aagcaacttc accaactggc gttccttcaa
    10681 gttcctccgc taagccacac aaccgattaa tttcctcaat tggcgtatac tcatcaatct
    10741 caaagggaag ttcataatca tgaattgcat attcctcgta ttcgtcattt aacccaattc
    10801 gttctttcac ttcatcaaaa tcgacaggtg gggtaaacca cgcccccacc aattcacctt
    10861 cgttatattt cccgaggttc gcaatataaa cacgcatctg ttccatcgtg cttccccctt
    10921 acgcaccgac aattcggtta aacaaactga gtaagacatc cttcacccca gaagcattaa
    10981 agaccaatcc gaccgcaatc aaagcaatca ccaaaaagcc aatcagtttg gaaaactccc
    11041 gcttaaatcc taaaaacaaa ccaatgacca caattgccag taagactaag gattgggcat
    11101 tggttaaaaa ccattgatat atggataggt aagttcctga tattataaat atctcaaact
    11161 tttcccccca ttcacacgga gcgtgcgact ttcaccgcac tccgcgttcc atcgtgttta
    11221 tattgaaaag tatttatgta gtagacctat atcaagttta attcttgatt gccagctaat
    11281 actctatatt tatttagtct tatgagctgg actttattta gcttcaatag agctatatat
    11341 ttttgtattg tttcttctgt tttagcgtgt attagacggt gtaccgcctc ttttaccaaa
    11401 ataagatttt tataatcgtc atttccacca gctccagttg gtacaatatg atgacaatgt
    11461 atttcattag cttgttgaaa ttcttccaga gttatagcac attttccatg ttgagcagaa
    11521 aatagcgaaa ttcgattgtc tgcgtattct attgtgcgat tttccaaaga taatttcatt
    11581 aactggtaca tcagtaattt atcaacactt agattggtgt ggataaattt tcttccctct
    11641 agagtgtagc aattcgctaa atacaccttt gccattggtt ttctgtgttg gatatatcca
    11701 acagggaaaa taggttcgcc ctcgccagca acataccgaa gcattttaga tttaccatat
    11761 cttgttgcct cctttgtcgt aagttgtctg cctgtttttc tcaatctgcc ccttttgttt
    11821 gtacctaatc tgttagtgaa gatagccatt acagctctat tcagtttcat acaatccaga
    11881 tttacattcg ttgcaatctg ataataattt tgcataccta ataccattga attataaagt
    11941 cttgtttctt ctaattccgt tttccctttt gagggagaag caattttctt tgcctgtgat
    12001 accagcttgt ctttttggtt tttgaattgt ttatcactta tatgtgattt aacaactaac
    12061 ttctttcttc tcggtatcat tttaatcttg aaaccaagaa agtcggagta tctttttcta
    12121 acatttacaa ttcttgtttt ttcttgtgat acttccaatt ttaatcgttg ttcaatccat
    12181 tgagtgattg ctattttggc tttttcagca ctttccttgt agcggcagaa aactctaaaa
    12241 tcatctgcat aacgaaccat atacatttcc tttaactttg ttttcttcat ttctttgtaa
    12301 gcgttgcttt tacatgggct accgctttta ttgatatgaa ccacatagtt ttttgtaaca
    12361 ggattttcct gccattggct ttctacccaa tggtctaact cattaagaac aatgtttgct
    12421 agtagtgggg atattattcc cccttgcgga gttcctttct ctggatatgt tattgtaccg
    12481 ttagtcattt tgattggagc ttttaatatc ctttttaaaa tgaatatcaa gtgcttgtcc
    12541 cgtattccca tagcccatat ctgttttatc agcttggaat ggttcacgtt gtcaaagaaa
    12601 cccttaatat caaattcaat cacataatgt agctggctga tttgtaggcg gttataggtt
    12661 gcttttattg cattttcaac tgacctatta ggtctaaaac catagctgtt ttcactaaat
    12721 ttagcttcgc agattggttc taatacttgt tttatacatt gttgtataag cctgtcccac
    12781 atacaaggaa tacctagagg tctggtttta ccatttggtt ttggtatttc ttttcgcctc
    12841 actggcttag gtcgataccc atgtggactg cctgcaacaa tgtatcttac ttttttgaca
    12901 actgtttctg ctggcaattt gccaatatct ttaattattg taccgtctgt tcctgctgtt
    12961 ttactgcctg catttgcttt gatatttcga tatgccagta atatattttc tctcgatagg
    13021 ataatatcca taagatgagt gaaaatatct ccattcttac ttttggcata taaatcatca
    13081 aaggtgtttt gcatttcgta atattcagcg tgtcttaaat cttctacaca caatgtttca
    13141 tttttatttt taggcataag gcttcactcc tttacttgaa attgagtgag cttttttatc
    13201 ttactcgtaa tccttattga aacaacataa tacttttcta atatatacac gacttgaggg
    13261 cattcctcca ttctcattac aagaatttct taggttcgcc ctctgctttt cagcattagt
    13321 tcaactgctt attgttcttc gtcagtttat tctcttgatg agaacccaat acctttcctc
    13381 gttccgataa tcttatctat acatatatat ccttaggtca ctagctataa gcctgctggc
    13441 ttgataccgc ctgtaacgtt atatggcatt tcataaccta tattcttact ttgccacacc
    13501 agctacgatt tctcgtacct ttgcatttct acaaagttca aatttagacc cgtacattcg
    13561 ctgtttcgtc agttccttat agaacattct aaccatagat attttataga cccccgacct
    13621 gttggatacg ctacccacct ctggatagtt ttcgatagaa gatatatgtt catcaactat
    13681 tacggtatct ttcagccgtc tttaccgagc ttcaaacatt ctgagtttcc tcatacaatg
    13741 cttgtcggag gctcagggca ggcgtttcag agcgttactt catcattcca cctgtcagat
    13801 tatcagttct cctaataacc aaactttttc gttcttttat tagtgcctta gcttttcact
    13861 aaggaacgag tcgcacataa attttgtccg aaattcatct tgtcactttc ctttcttttc
    13921 cattgctcga tccgctgctg cacttgcatt catcaagcac atcacagtaa caccaagcat
    13981 actgccacca aataccaaaa aaatatcttt tacaattccc atgacgattc ctcttttcat
    14041 ataatcaaat tttcaattgc catactttgt tgctccaaaa tctttttatg tcggtctgat
    14101 aagcgtgcat tctgtaccat atcctggata taggttgtcc gattcaatcg atccaactcc
    14161 ttagccattt tccacgtagg ggcgacttgt cggcctagcc aattcaaggt tcgttcaatg
    14221 gtatacggtt ttggctcgga agttaattga atctctttcc gattccgtcc aataaaacgc
    14281 ccccattgtt cattcaaggg ccagttggtt cgccttttac cttctacttt atccgcaaat
    14341 cgcaagtaac gattgataat ggaaaacgtg gtgctctcaa tgctacgacc ttttaacaaa
    14401 tcttgaatcg catgaaaagc acggtcattt ttcagtcgaa tctcaaaacg atttttcgtc
    14461 tcagtttggt ctaatggaat ccctaacttg atatattgtt catagtcttt ttcatacaaa
    14521 cagaaataaa cctcactctt gagactgccg atatataagg tattgcccat gccgtccttt
    14581 tcatcagcct ttaaaagttc cccggaacga taacttttaa aggtacgaaa caaggagata
    14641 cattcttcct tctgacattt tttcgttagc tcaggtatat ccaatagccc cactcgatca
    14701 ttgattgcca agtccaagcg tttcatcacg ccaccggcct ttagacaatc ctcgaaaaag
    14761 tcgtaccagc tacgcttttg agcgagtaaa aaagtttcaa attgtcggca acctcgcccc
    14821 tttaattcta aaagcacacc tttatcttct tcatgagaca acatcaccac aatatcaccc
    14881 atgacatact gttcttgata ggagtaaaag gcataatctt catggagcat atactcaaat
    14941 ttcaaatgga gaatttcttg aataattttt cgtgcatccg ttgtgggaaa gcgaatcctt
    15001 acatagtcaa acagtaacgt caaaggcaaa tcgggattaa agctttccag ttggtcaaaa
    15061 attttcctca tggtttttat agatgcggtt acgccaccat tttctatctg atttaagtac
    15121 ggccgactaa gtccacaagc cgtagccaat ttattttgtg aaacgccgta ttcgttgcgt
    15181 cggtctttta atagccgata atctaaattc cgttgtgcca agttctcacc tccaaattca
    15241 aggtgttaac tttttcaaat gagttaacac ctatacaaat tacttactat ctttgataca
    15301 acaagcattc ttaatggttg cttacttatt ttttgtgttt ttgtaccccc ctgttagata
    15361 cgggggtgag acatcatggt cggctaacgc cgtccatgaa caggcacagc accacggagg
    15421 atttctgctt cgcgaatcct ccgcggtcag cgcctaacag tcagttgtgt tggtttaggt
    15481 tactcatctg taataccatt tacttccaac tctgctttca tttctaacaa gtattgacga
    15541 atatctggtt cttttgcttg gtccgcaatc cgctcaaatt ctgcaattac ttgatttatt
    15601 tcaattcgag catccgtctc ctcattcatt aaatatggat cgccagcgac tttgacacca
    15661 ttcttgcgaa tatcaaattt taaccgtcgc aattgttctt ctaattctgg gtctgactga
    15721 atctttccaa tcacccaata aatttctcca atcaactcat cataaaccat gtacatccaa
    15781 ctcttttaat cttctggcaa tatcaaaaat gaccggaact gttacagagt ttccggcttg
    15841 cttatacaac tgactatctg aattgacttg agaatctcga tcaaaagccc aatcgggaaa
    15901 gccctgaagt cgccaacact ctctaggtgt tagcttccga atttgatact gatccgtgac
    15961 gatcgcttgt tggtctcctg ttagtaaggt atgaacactc tgttttccca gacgacctcg
    16021 gcgacttgtg gaattaggaa aagccagatt cacactgtct tctggcttca cctcttgata
    16081 ttctgtttca ttttttcgaa tcatgatttt tgtaccacct tgtttcgttg acaaggtggg
    16141 ggcggttcca gtaacttcat aaactcgatt accttgttca aactttcccg gtaatagtcc
    16201 tgctacagag attccatccc ttgctggcag tgcgattttg attccttcat cacctgttaa
    16261 ggttggcgct acgccatctg aaccatagac ttgtccaccc attcctcgat tggaaggatt
    16321 gatatttacc aaatttctga tgttttgatc agatgttgaa gacggtccaa tgataggaaa
    16381 tacttgttcg gtacatgcgt ctcgagaatg tccgataagg aatatccgct cccggctttg
    16441 gggaatgtag gcctttgagt taagcaccta ccactccacg tcatacccca atccatccag
    16501 cgttcggagg atcgtctcga acgtagcccc tccttcgtga ttgagcaatc ctcggacgtt
    16561 ctcaaggaat aaaagcttag gtcggagaag agtggtgaac cgagcgattt caaagaataa
    16621 agtacctcga gtatcggaaa atctttgccg ctttcccgca attgaaaaag cttggcacgg
    16681 aaatccgccg caaaggatgt ccactggtcc gagggattga ataaactcat ctgatacact
    16741 tgtgatgtca tgcatttcca cctccttact tgtatcatga attgctttat aactacgtct
    16801 agcaaattta tcaatttcac aaaaaccgat acaatgatgg ctcgcctgtt ccatacctaa
    16861 acgaaagcca ccaataccgg cgaataaatc taaaaatctc atacattacc tcattcattg
    16921 caaagaaaaa agccaagaac attttgctct tggctcatac attattaatt tgtcctacta
    16981 ttttttattt aatccaacta atttccgttg tttttcattg gcctgatcgg ctacatattt
    17041 aatcaccgtt ttgaactctt ttgcagtagc attttccgat aaatccgcaa tgctcttatt
    17101 gtatttcttt acaattttcg ttacttgttc tttctcagtc atgtaattga acctccaaat
    17161 ctttcctact ataccctagt ggtaaaaata attctagatt atccgtagca taatgaactt
    17221 cggaaatatt attcgtataa tcttttatta tgatatgaag caatacatct ggatgtttac
    17281 tctttacttt tgcaatgttc tctcttgatt tgaaaaaagc gttaataaat gcctttttcg
    17341 gcacatgacg tccctctgca atttcacgtt cttgtgtaaa tttccaagcg attttagggt
    17401 cttgataaac atagaaaatc gtcactcgaa aatgtttttt caacgcccgc gagatatttt
    17461 tttcagcact taatattgca aaagttgcat ccagaataaa agaatagcca tgatctaaga
    17521 catatctaaa tgcctgctct accaaccaag aagatgcttt ttgaaagaga ctagagtttt
    17581 ttccattata atcaggaaac tgggtgcgaa agtaatccgc atcaataatc acatggttat
    17641 cataattctc agctaatcca atggcaacct ctgattttcc agccccagga ctccctgcca
    17701 taaaaactgc gtctcgtcca acttcggggg atttcccttt gataatataa gaaataaact
    17761 tatccttatt tgcttttgca tactctaaat aagaatcatt actttccata ttaattatcc
    17821 cctttcttcc tactatataa aagtattata cgttacttga agtataaagt cagtattgaa
    17881 gatcactcaa tcattcatct tttctaattt actaattgaa tctaagaaat catacccttt
    17941 tggtacaagt ggcgtataaa actcactaat cacactgcca cctgtatcca cataacctcg
    18001 acctttgatg tgcttcataa agaagttttt ttcgacctcc ccaaacatca tggaatatcc
    18061 gagttcactc atacgaccca aagccacgcg aaagttgaat tgatcgcgga ttccgtctcc
    18121 taaatatttc gcatccggcc tttggcaagc caaaatcaga aagaaaccag attgacgacc
    18181 caacatcacg atttgtttga gcttattcaa aatcaccgca ctttctttcg tcgttaacat
    18241 ctccatataa gccacgtatt catcaaagat taaaaagttg ggtggaagtc caagataggc
    18301 ataattctct cctgttttgt agttagacat ttctttcatt gccttgctac gagccatcat
    18361 acgttcatag aaatcttcca cacaagcaga aatttcttcc ttttgtgaat aaacatgagg
    18421 catcaccgta cctaaatcag ctaaatctgc atttttggga tcgaggataa acaattctgc
    18481 atctgacttc aatagtgctt cgataatggt gaggaggaaa taggtcttcc ctccacccgt
    18541 gccaccagcg attaacatat ggggtaaaga atcataagcc catacttgat ttttcatcaa
    18601 tcgcagagtc ccattttctg ccactacttc gtctatccca attcgattgg cgatcatatc
    18661 atacagtaaa gtatactcca cataggagtc tttgagttcc ttttctacta actcacaata
    18721 cagtccgctt tctaacttct tttccaattt taaaagctga tcttggtatt tcccaagtga
    18781 aatttgaacg cgaatagaca gtaatccatc tttcattcga taacagattt tggggaagta
    18841 actgatagtc tctctggttc ggctactatt caaatctttg aaaaagcctt cactttgagt
    18901 ttgtttcact tcataccaat ggttctctag aatcatccga gctagctttt gtcgatgcca
    18961 caagcttcgc caatgatcaa gaaaaaagtg aatataaagc aatatcatag ctcctgaaaa
    19021 agcgagactt ccaagcagac atactaaatc ttttatagac caaacgattt caatagtaga
    19081 aagtgatagc ttagaaatat caaattgtct aaactcttga aaataaaaga cgctcatcca
    19141 accgatgaac agagcgagta gccagccaag aacaaagtga taaaccaagt gctgatctcc
    19201 tgcgcgtatg cgatgtcctt tgtacatttt gatggtttcc acctccaatc gaaaaagccc
    19261 aataggattt cattaggcaa tttcaaaaag ttatgctagt catttactgc tacgaggagg
    19321 ggagttgttt cgtttcaacc aattgattta aaaagccttc aatagcatcc ggcatctcca
    19381 caccaatttc ttccaaaatg gattctgcct gccgacataa gttttcattt acttcgacaa
    19441 ctataaagaa agactcttct tctcctaacc agtctagtac atcagagtct tcttcactgt
    19501 cgctattttc tccaagtaaa ccttgatgta taatcagaaa cattttttca tcagaggtta
    19561 atgctttaac gagttgttcc tcagtcagct cgccttttcc atattgatcg agacaatcta
    19621 aaatatgttc aacattaatt tccataatct tcacctattt ctttctttca ttttggttcg
    19681 taggatttcc ttgattcggc tgccgcttca agactaaatc atccgcctta atgaaccaat
    19741 taacggtagt tccttctcga aaaacataat tggcaacggt atccacgatt ggattgacca
    19801 actctacctc tgcatcataa gcaaactcgc gtaagggtac attagctggg atactaactt
    19861 gaatcattcg cccttgtgca gaggatttta aattataggt tcgttctttt acctcattac
    19921 caattgttcc atcctcattt tgcttccgta cttctcggcg taatgcggaa aaacgcaact
    19981 tcccaaatgt taaacctgaa tctacgacaa taccttctgt taatctcatc ttcttttcct
    20041 cctatgcttt cactaaatca tcaacataca acacgtaatc agtaaaccca cgattctcaa
    20101 tctcatagcc tttggcaacc aaatggggat tcacggcttt tagcggttgg tcttgatcaa
    20161 aatccttttc accggcttcc gcagctacta ccacctcaat atcatcggcc cgttgcttac
    20221 tggaatacaa atgataactg cgaccaatca ccgtagtatt ccgtccatat ccttctgtta
    20281 taatttctcc ttcaccggca taacttaact ttccaaaggt ttcagccata tctggcacga
    20341 caaatttcaa ttccatcttc ttttcctctt ttcattgtta gttttggata caaaaatagc
    20401 gcgactactt ttttaggagg cggtcgcgca cgttggttct taggatttac ttgtatcatc
    20461 tcatcacctt agttccggcg attccagaaa taatagcccg ccgtcgcaaa aatcaaagta
    20521 aaaccaatga acagtaaaac attggaattt ttctccccgg tttgagggaa ggtcttcact
    20581 gtgggactag attcttgaga gccacttgga acagttggtg tttccggttg tttcggcgta
    20641 cttagtatgg ttggaacttc ttttggtgtt aaggtttgag atttttcttt caaatcttca
    20701 ttgtgttttc cattgatatt cccatcttta tcgtaattga tttccgcaaa agtaaacttg
    20761 gttccttctg gatattttcc ggtatctact tttttcgcaa gcacagtttt ggtgaattct
    20821 ttatcattta cttcataatc aatcttgcca gatttccaaa tttctttgtt tgtaccatcc
    20881 ggtaataaag catacagaat tgtttcgaaa gcttcttttg agccatccag tacatcatga
    20941 gtaatcgaca catcatcaaa catatccacc acatcaccat gagtaaaggt ctgagaaccg
    21001 ttttctaggt gggcctttgt ttggatagaa acatggtgtt ccactgtaca actgacagtt
    21061 tgggcttggt tattcaagct aacatccttc gctactggct tatcgcctgc ttcaaaagct
    21121 tccttatttt catacacata gttgaataag acaatagttt tatttaaaac ttgttcggcg
    21181 gttaatttat gctgaatttt ccaagttcct gttttctctt tgttcgccaa cgttgcgctt
    21241 gtttctgcaa tcaccactgg tttggctttt tcatcttttt cttgggcagc ttttgtcgca
    21301 tccacatcaa tggcttgcgc taccacatac cagtcttctt tgatttcatg tagattgtaa
    21361 ctcaatttat caactagctc ggtagaatcc agagaagtta attctttgtt tccgtctttc
    21421 caagtcgcaa gagaagttaa actatcttct tcctcaggca aatcatagag cattaaacgg
    21481 ttcaaactaa cagaaaatgc tttgttcgtc agcttctcgt aaggaacggt caccgtttta
    21541 attggttgtt tttggtcttg ttcggtaatg gtaaagacat actcactctt cacaaagtct
    21601 tctttgtttt ccttaaaagt agaacggatt tctaatggcg taatcttttg aaaaccttct
    21661 ggggcttcta cttcttcaag taaataatcc ccataaggaa gattttcaaa cttgccataa
    21721 ccatcaaacc ctaattgctc gttataagct gtggtcgctt catcttcggc acctgtgatt
    21781 tcattggtcc cttccaatgg cgacacttta aaggtcagat cattgaagcc agtttcggta
    21841 gtgcccgcag ctgatccagc aaatttaaag aaatcaaagc caaagcgaat cacttgttct
    21901 tttgccgtaa catttcgggt aatcacggca tttttttcgc tatcatccac ctttttgatg
    21961 gatacaggat actttgtttc atcaaggcta tatccttcgg gcgcttttgt ttcttgccaa
    22021 taatactcgt tgatagctaa atgtttaacg gcaacttggt tcttttcgtc taaagtcaaa
    22081 gtgaccgttt catcagaacc tttcgttccc ttcgctaact ctggtttaaa agcctcactc
    22141 catttaacag cttttccatc cttagcagta aacaaggtgt attcagcacc ctcaaagact
    22201 gccttacctt gtgctttatc cccagtatct ttgtcttctt tcgtcaaagt ggtttcgcca
    22261 gtgacttctt gattttgccc ttttacgtta ctggtaacag gggctacggt ttgattggca
    22321 tacttcaatt cgacttttac aggtttgaac gtattcacga acccatgact agctttagtt
    22381 tcggtcacat aataggttcc caattccaac gcattagcaa tctcttttgg tgtttccgca
    22441 tgaccgtttt catccgtggt catttcttga acgatttcac cggttggact gtctttgcga
    22501 atggcaaacg tattaccagc taaggaataa ttgtcattcc aaagatcact ccctgtttca
    22561 actccgcttt tatccagaat gatttgccct ttttcccgtg cgttttttga agtcacgtaa
    22621 atggtttcgc ctgctttaat ggtggtagtc atgggggtgg tatcaatcgt ataaggcgcc
    22681 ggtactgatt tttcggtaat ggttactttt gtaccatgag gaatctcatc caatgtagca
    22741 atgccttctt tgtcagtagt cacgtctttt gcaggtaaag tttttccaaa gtctaaatgg
    22801 aaaaccgttc ctggtacaac atcacccgat tctttatcga cttttttgat ctttaaagaa
    22861 ccctccgttt ccacatcaat tttgattgtg taggtattag gtttatcaat cgccccggcc
    22921 attaaagtct gttggccagc ctttttatag gcgacaggtg ttccggtacc agcagatttc
    22981 ttaagcgtca gcacaccact ttcttttgaa ttggcatttg gggtgattat caactgatta
    23041 ccattcacac gataatcaat gtttgcggca ttttccacaa cctcatcgaa ctctgacaaa
    23101 ttcagattat tcgtatccgt cacagtagtc gattgcccta atactgtttt agccgtacta
    23161 ttatggaagg ttggtttctt ctgataatcg tcaatggctt gatttatttt tgcctcaatg
    23221 gctgcgatat ttacggcact accatctgat cgttttattg agtgaagggt atacccatta
    23281 acttcttgcc agatcatctt ctgtgccaca atatgagtat ctgcatctgt acctactttt
    23341 ttccataaaa cggaaactaa tttggcttta tcggacatat ctggtaacgg gtttttctca
    23401 tagccaggag tgaactcatt gtaaatcggg actcctggtt caatacagaa aatatcttgt
    23461 ttaaagccgt tgtacactgc ataatgcgga acagtgactt ctgacattgg acgaccatca
    23521 ctaaaagttc cttcaacagc atattgatgt gctaaatcta gttctacggt taccgtttgt
    23581 ggatgggtaa tttcatcagc aaaagtaatc gtaggagcaa ttgctccaac aattgcttga
    23641 acagccaaac tcaaaatagt taatacacat aatttctttt tttgtttttt atacatgaaa
    23701 tgttctcctt tcaaattaag cacaaaaaaa taagatgctc gcttgaacat ctttgtttat
    23761 aaagtctttt taaaattttt attgactgaa tagaatggat ttgagaatga atgagcctaa
    23821 aaatttaatc taaaat
//
LOCUS       DAZKLM010000039        23647 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3764
            SAMEA115706913-rid22582563.denovo.039, whole genome shotgun
            sequence.
ACCESSION   DAZKLM010000039 DAZKLM010000000
VERSION     DAZKLM010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706913
            Sequence Read Archive: ERR13196647
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23647)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23647)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287823.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287823.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,004
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,849
            CDSs (with protein)               :: 2,849
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 41 of 96
            Pseudo Genes (incomplete)         :: 52 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 15 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23647
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706913-rid22582563.denovo.039"
                     /isolate="VRE3764"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(461..1090)
                     /gene="udk"
                     /locus_tag="ACG842_001409"
     CDS             complement(461..1090)
                     /gene="udk"
                     /locus_tag="ACG842_001409"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004849 - uridine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HFQ0247959.1"
                     /translation="MTKSKPIIIGVTGGSGSGKTSVSRAIFNNFPDHSIMMLEQDSYY
                     KDQSHLSFEERLNTNYDHPFAFDNDLLIQHVGDLLNYKAIEKPVYDYVAHTRSQATII
                     QEPKEVIILEGILILEDERLRDLMDIKVYVDTDDDIRIIRRIKRDMEERGRTLDSVIE
                     QYLTVVKPMYHQFIEPTKRYADIIVPEGGENHVAIDLITTKVASFLNHK"
     gene            complement(1174..1869)
                     /locus_tag="ACG842_001410"
     CDS             complement(1174..1869)
                     /locus_tag="ACG842_001410"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjjG family noncanonical pyrimidine
                     nucleotidase"
                     /protein_id="HFQ0247960.1"
                     /translation="MKYKTLLFDVDDTLLDFQLTEANALSALFAEQGITLTPEIETSY
                     KALNHHLWQEFEKGRLSREEIIGSRFGLLFQQFGKTVDSDQMEKRYRHYLNQGHDLVP
                     GSLEILKKVHPHADLYIVTNGVSHTQHRRLTDAGMLDYFKDIFVSEAVGAQKPMKEFF
                     DHVFSHIPNFDKKQTVIIGDSLTSDIKGGNMAGIDTIWFNRNKLPEIEEIQPTYRIDS
                     LEELYSLLEIEKD"
     gene            complement(1882..2598)
                     /locus_tag="ACG842_001411"
     CDS             complement(1882..2598)
                     /locus_tag="ACG842_001411"
                     /EC_number="5.4.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0140098 - catalytic activity, acting on
                     RNA [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase"
                     /protein_id="HFQ0247961.1"
                     /translation="MRLDKLIEEKLETTRKEMKRLFALKQVMIDGTVEFRQNRNVDSL
                     LHQIEVAGEHLETNESYYLLNKPEKAVTAVSDPEKTTVIDLIASADRFQPLYPVGRLD
                     RDTTGLLLITSNGQLGYEMLLPEKKVSKTYQVVVNERVTPADVTAFENGIVFADGYQC
                     KPADLKILKADKHQSTVLLTIQEGKFHQVKKMFLAQGKKVTALKRLSMGPLELDEQLG
                     VGEYRSLTLAEMKKLSIYFK"
     gene            2928..3536
                     /locus_tag="ACG842_001412"
     CDS             2928..3536
                     /locus_tag="ACG842_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HFQ0247962.1"
                     /translation="MSVSLEVSKREVRPRSLRNKLRHEGKVPAVVYGYKVESTPISFD
                     GLTLSKLLREHGANTVITMDIDGQKVNTLVHKIQTNTFTNHFEHAEFLSVNMSEETEV
                     ETEVVLVGESVGAKSGGVLAQNLYTVVVSATPDKLPERIEVDVTNLELGDSITVADLP
                     KNDDYKFVTDSEEQIAAVVAQSEEEESTGEGGEPEVIGSTEE"
     gene            3861..4184
                     /locus_tag="ACG842_001413"
     CDS             3861..4184
                     /locus_tag="ACG842_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HFQ0247963.1"
                     /translation="MFESFDSSRNRYASVGVVSSLPDELIDSIWFIIDLDLKGVIPLE
                     NILTFDLIENRGKVTMHFSQAGSNVEMDIDLPFAYSNQFPAQVYAYDDGTRETILLPS
                     EIRQY"
     gene            4366..5736
                     /locus_tag="ACG842_001414"
     CDS             4366..5736
                     /locus_tag="ACG842_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HFQ0247964.1"
                     /translation="MRDLTKGSPAKLILMFTVPLLIGNVFQQFYNMIDMIIVGQTLGK
                     NALAAVGATGSLTFLIIGFAQGLTAGLAIITAQRYGAKDYRGLKKSFAASVVISLVVT
                     VILTVLSLLFIRPMLQLMQTPPEILDQAQTFISIILLGIFASVSFNLLSNVIRALGDS
                     RTPLFFLIIAVIINVVLDLIFIIYFGMGVEGAAIATVIAQVSSSVLCLVYIKKKMPLL
                     QLRKKDFKFEKEEFAVHLNAALPMAFQSSIIAVGAIVLQAALNSLGTDVVAAQAAAGR
                     IDQFANQPMMSFGIAMATFTAQNYGAKEYGRILQGVRQTLLMSVGFSLLAGAIVIFFG
                     HFFVQLFVSPSETRVFELAQTYFNINGSLYWILAVLFILRYTLQGLGQSKIPTIAGMM
                     ELLMRSFAAIVLTGMWGYPGAAAASPLAWIGSVAVLLYSYMRSMKQLKHMGEEEHFTL
                     EHAEAR"
     gene            complement(5866..7167)
                     /locus_tag="ACG842_001415"
     CDS             complement(5866..7167)
                     /locus_tag="ACG842_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HFQ0247965.1"
                     /translation="MVINFIILFLMIAITSFFVASEFALVKIRRSRLEQLEKENVKNA
                     KLALHVTHHLDNYLSASQLGITLTGLIIGWVGEGSVAALLEPVIGKLPFSSAISSTIS
                     VALGFILVTYIDVVIGELLPKSYSIVNTEKVVLFVVKPLHYFYKVMFPFIWVLNHSAA
                     GLGKLLGVRLVSEGEETLSQEELTLVALNSYEKGELTKEEYYYLENVFEFDDTLAKDI
                     QVDRTSMQVFEASETVKEAIKASIEQGHTRYPVILESKDNVLGYVTLPDLIKQSYKDD
                     QLTVEQLIEEPIVTTETIPIKKLLTIMRKKGKHIAILKDEYGGTSGLVTIEDILEEIV
                     GEIRDETDLDEALIAEQSDGSYIISGKLTLDDFQRYFHVEIPEFEETNFTTLAGFASS
                     RYKEIKAGTIIEIASFRFTVLEYQHAHIDYFKVESTERKTE"
     gene            complement(7521..9512)
                     /locus_tag="ACG842_001416"
     CDS             complement(7521..9512)
                     /locus_tag="ACG842_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HFQ0247966.1"
                     /translation="MATTHNTKKISMTGLLVAMGVVYGDIGTSPLYVMKSIVEDNGGL
                     SGINPDFVIGSVSLIFWTLTLLTTIKYVVIALNADNHGEGGIFSLYTLVRKGGKYLII
                     PAMIGGAALLADGVLTPAVTVTTAIEGLRGIPQFFDRFGNNQNVIVIITLVIIFVLFM
                     VQRFGTDIVGKAFGPIMFAWFTFLGVMGLMNFSQDWTVIRALNPYYAIQLLFSADNKL
                     GLFILGNIFLATTGAEALYSDLGHVGKRNIRLSWPYVKICLVINYFGQAAWLLNVYQN
                     PEAQQIKNLNPFFQMMPQSWIVIGVAFATVAAVIASQALITGSFTLVSEAIKLKLLPR
                     LKIMYPGNSIGQMYIPAVNLILWIACSLVVVTFRTSHHMEAAYGLSITVTMLMTTALL
                     YFYLIQNGHSRWLAYLVTFFFGAIEVVFFISSVVKFFRGGFVAVLIGLIILVVMFIWE
                     QGNIIRESSTEDVSLRDYIPQLRELRDDTSLPIYQTNVVFLVPDMAEDKVGRQFIYSI
                     LDKRPKRARVYWFVHVEVTDEPYTKEYQVDMMDTDFIVQVNLFLGFRVQQEINVYVRQ
                     VIHDLMKQGRLPKQPQRYSLTPGREVGDFQFVLIDEVVSNVTTLGKWQRQIMQAKLAI
                     KKIATSPETWFGLEYSEVKHETVPLIIGNTRKTWLKERL"
     gene            complement(9656..9922)
                     /locus_tag="ACG842_001417"
     CDS             complement(9656..9922)
                     /locus_tag="ACG842_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFQ0247967.1"
                     /translation="MLNFLWSLFIGGILGLIAGAILGKEVPGGVSGNIIIGFLGSWLG
                     EFLLGPLGPELGGFYLIPALLGAIFCLALYSFVARRIRERPKKE"
     gene            complement(10432..12558)
                     /gene="pnp"
                     /locus_tag="ACG842_001418"
     CDS             complement(10432..12558)
                     /gene="pnp"
                     /locus_tag="ACG842_001418"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HFQ0247968.1"
                     /translation="MSEKQVFKTTWGGRPLQIEVGQLAKQANGAVLVRYGDTVVLSAA
                     VASKEAKDTDFFPLTINYEEKMYAAGKIPGGFIKREGRPSTEATLTARLIDRPIRPMF
                     AEGFRNEVQVTNIVMSVETDCSPAMAAMLGSSLALSISDIPFDGPIAGVEVGRVNGEY
                     VLNPTVEQAEQTDIELTVAGTKQAINMVESGAKEVSEEDMLGALLFGFDAIKELVAFQ
                     EEIVQAVGKEKMEVTLLQVDEVLKKEIFDASYATMKAAVMTEEKLAREDNIEQVKIDI
                     REAYAEKFAGHEDEDHLLKEVKQITEDLEKDVVRELITIDKIRPDGRKLDEIRPLSSE
                     VSLLPRVHGSGLFTRGQTQALSACTLAPLGEHQIIDGLGVEVSKRFIHHYNFPQFSVG
                     STGRAGSPGRREIGHGALGERALAQVIPSEEEFPYTIRLVAEVLESNGSSSQASICAG
                     TLALMDAGVPIKAPVAGIAMGLVSDGENYTILTDIQGLEDHLGDMDFKVAGTKDGITA
                     LQMDIKIQGITEQILTEALTQAKQARMEILEELTSTIAAPREELSQYAPKIEMIQIEP
                     AKIKDVIGKGGDTINGIIDETGVKIDIDQDGKVSIASADAEMIKKAIKIIEDLTKEVK
                     VGEVYLGKVVRIEKFGAFVNLIKGKDGLVHISQLANDRVNKVEDVVKLGDEILVKVTE
                     IDKQGRVNLSRKAMLNEDGSTKNEEK"
     gene            complement(12746..13015)
                     /gene="rpsO"
                     /locus_tag="ACG842_001419"
     CDS             complement(12746..13015)
                     /gene="rpsO"
                     /locus_tag="ACG842_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HFQ0247969.1"
                     /translation="MAISKERKNEIINEYARHEGDTGSPEVQIAVLTEEINHLNEHAR
                     VHKKDHHSYRGLMKKVGHRRNLLAYLRKTDVQRYRELIKRLGLRR"
     gene            13266..13829
                     /gene="def"
                     /locus_tag="ACG842_001420"
     CDS             13266..13829
                     /gene="def"
                     /locus_tag="ACG842_001420"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HFQ0247970.1"
                     /translation="MITMDDIIREGNPTLREVAKEVSLPLSEEDISLGKEMLEFLKNS
                     QDPIKAEELHLRGGVGLAAPQLDISKRIIAVHVPSPDPEADGPSLSTVMYNPKILSHS
                     VQDACLGEGEGCLSVDREVPGYVVRHAKITVSYYDMNGEKHKIRLKNYESIVVQHEID
                     HINGVMFYDHINDQNPFALKEGVLVIE"
     gene            complement(14082..14603)
                     /locus_tag="ACG842_001421"
     CDS             complement(14082..14603)
                     /locus_tag="ACG842_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HFQ0247971.1"
                     /translation="MIEGETRRKQIMQTLANTEKPVSASKFAKTFGVSRQIIVGDIAL
                     LRAAGKSIVATARGYILETEETNTGHISKIAVQHGKDQTEEELRLIVENGGEIIDVIV
                     EHPLYGELAGTLHIKTPQDIDSFMRRYQKSNATLLSELTGGIHLHTIRYPEKHMLKNI
                     KQKLAEAGILYEG"
     gene            complement(14606..15448)
                     /locus_tag="ACG842_001422"
     CDS             complement(14606..15448)
                     /locus_tag="ACG842_001422"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HFQ0247972.1"
                     /translation="MTEFKTEKEKMIAGELYFANDPELVADRMFARSQSQIINQAESA
                     ELRSQLLKETFGRTGKKIYMEPVINFDYGYNIFVGENFYANFNCTFLDVSTIEIGDNC
                     MFAPNVQLYTATHPLHPVKRNSGLEYAKPIKIGNNVWLGGGVIVTPGVTLGDNVVVGA
                     GSVVTKSFPDNVVIAGNPARIIKTVEEELTEESLETLRHKIDMIDRELIALLEKRMDT
                     VTKIGQVKKEENQAVYDAKREQQVLDKVVSLLKNKGYKETITDTYVDLMKHSREYQNK
                     MKEE"
     gene            complement(15441..16166)
                     /locus_tag="ACG842_001423"
     CDS             complement(15441..16166)
                     /locus_tag="ACG842_001423"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0034979 - NAD-dependent protein lysine
                     deacetylase activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HFQ0247973.1"
                     /translation="MKQLTVDEAAKEILAYQQITFLTGAGVSTPSGVPDYRSLSGIYH
                     GMEQPEYLLSHQAMVNEPDKFYQFVKKIYHPEAKPNIIHLEMAHLAKEKNVWVVSQNI
                     DGLHAKAKSPQLVNFHGSLYQCHCRKCQQTVDWKEYLHSDKHQACGGQIRPDIVLYGE
                     GFQDGVMEQAAYAVSQAELIVIVGTSFQVHPFCDLVQFRQPSAKILVINQTPVYLAEP
                     YTFIQEDGAVVFKKIQELGADYD"
     gene            16342..16638
                     /locus_tag="ACG842_001424"
     CDS             16342..16638
                     /locus_tag="ACG842_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0247974.1"
                     /translation="MLPFFIYWGVILACIAWLALSVYFSVFYLVRKENGNLWAFALFN
                     VIAAIVLAITLAVYRTWGWGITQYSSLIYLILAIYGVVVLLQAILGREPKKAAA"
     regulatory      16894..17068
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00168"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Lysine riboswitch is most abundant in Firmicutes
                     and Gammaproteobacteria where they are found upstream of a
                     number of genes involved in lysine biosynthesis, transport
                     and catabolism; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="lysine"
                     /db_xref="RFAM:RF00168"
     gene            17206..18798
                     /locus_tag="ACG842_001425"
     CDS             17206..18798
                     /locus_tag="ACG842_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327538.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015837 - amine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFQ0247975.1"
                     /translation="MKKNMVSVAVLFAFVFSLFSVGSLANADEKTPSDEFRVGMEAGY
                     APFNWSQQTDENNALPIQGQNSYAGGYDVQIAKKVADGLGKKLVIVQTKWDGLAPALQ
                     SGKIDAIIAGMSPTAERRKEIDFTDPYYESQLVVVVQKDSKYAKAKSLADLSGAKITA
                     QLNTFHYTVIDQIPDVQKQQAMDNFSAMRTALASGMIDGYVSERPEGVTATSVNKDLE
                     MLEFSGADGFQTNPEDVQISVGMRKGDPDIQKVNQILSGISNDERTKIMDQAVKDQPA
                     ATDSEEKPGIISDFTTIWKQYGNMFLRGAGLTLFIALIGTVVGTTLGLLIGVFRSVPE
                     SGNALTRFFQKIGNALLSIYVEVFRGTPMMVQAMVIFYGLALAFGISLNRTIAALFIV
                     SLNTGAYMTEIVRGGIFAVDEGQFEAAQAIGMTHGQTMRKVVIPQVLRNILPATGNEF
                     VINIKDTAVLSVIGVADLFFQGNSASGANFQFFQTFTIVGIIYLVMTFTITRILRAVE
                     KKIDGPSAYTKLEEIDNSNLQE"
     gene            18811..19551
                     /locus_tag="ACG842_001426"
     CDS             18811..19551
                     /locus_tag="ACG842_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFQ0247976.1"
                     /translation="MNEKIIEIKHLRKSFGENEVLKDISVTVNKGEVVTIIGSSGSGK
                     STFLRCINLLEKPTGGEIIYNGENVLAPKYNLPKYRTNLGMVFQSFNLFNNMNVLENC
                     MSGQTTVLKRDKETAKKVAIENLEKVGMDRYIEAKPAQLSGGQKQRVAIARALSMNPD
                     VLLFDEPTSALDPEMVGEVLNTIKDLAHTGLTMLIVTHEMEFAKDVSDRVIFMDKGVI
                     AEEGTPEDIFVHPKEERTKEFLSRILNA"
     gene            19719..21068
                     /locus_tag="ACG842_001427"
     CDS             19719..21068
                     /locus_tag="ACG842_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFQ0247977.1"
                     /translation="MNEEQELQRGLKNRHVQLISIGGAIGTGLFLGSGKSIQLAGPSI
                     LLAYLITGCICFFIMRALGELLLSNTNNHSFLDFVAEYLGKKAAFITGWTYWFCWVSI
                     AMADLTAIGMYVRYWAPGVPQWLPELIALGLLLCLNMVAVSLFGELEFWFALIKVVAI
                     IAFILVGAYMILTHYTTDIGTASITNLWSHGGFFPTGAKGFILAFQMVTFAFAGIELV
                     GLVAGETENPEKVLPEAINNIPIRIILFYLGSLFVIMAIYPWNSLNPDNSPFVEVFSE
                     IGITIAASLINLVVLSAAASACNSAIYSTGRMLRSLAQEGSAPKKFKKLTTHHVPGNA
                     LTFSTIVIFISVILNYVMPSEVFTLVSSIATTCFLFIWSMLVYTHMKYRKSILGKKAH
                     SFKMPLYPFSNYLVFLYMAFVCVVLFLGKDTRIALLLTPVWFLLLLLIYHMKYERKK"
     gene            complement(21196..21786)
                     /locus_tag="ACG842_001428"
     CDS             complement(21196..21786)
                     /locus_tag="ACG842_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071805 - potassium ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM175 family protein"
                     /protein_id="HFQ0247978.1"
                     /translation="MTKSRLEAFTDGVVAIVLRVLVLEIKIPNTSSFHSLWVIRNTLL
                     AYTISFIFVAVIWVNHHRIFQMAERINYRVVWSNIFLLFWLTLCPSVTSWVGRNPEAF
                     WPEMAYVLVYTMWSFSFGILMRQIMKANKPDSHVVRVLSKDRRSLISMMINLCLIAGV
                     FFVPLIGLFGRFFVSGIWIFSYKKADAYYQRLFPGK"
     gene            21933..23285
                     /gene="brnQ"
                     /locus_tag="ACG842_001429"
     CDS             21933..23285
                     /gene="brnQ"
                     /locus_tag="ACG842_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HFQ0247979.1"
                     /translation="MTKKLSFKDYLFIGSMLFGLFFGAGNLIFPVHLGQEAGAATFWA
                     NLGFLVTGIGLPFLGVIAIGVSKSSGVYELAKRINQTYAMVFTILLYLVIGPFFALPR
                     LATTSFEIGLAPFIDPSRQTLYLAIFSIVFFVLAWWFSRKPTKILDYVGKFLNPAFLV
                     LLGALLLLAFLNPLGSVNQAPIQPNYQEHAFFTGFTQGYNTLDALAALAFGIIIVTTI
                     QNMGVTKPAEIAKDTIKSGAISIILMGIIYTLLAYLGAMSLGSFALSENGGITLAQIA
                     DHYLGTYGSILLAFIVILACLKTGIGLITAFAETFTDLFPQRNYAFFVALASILPCLA
                     ANVGLTNIIQFSLPVLMFIYPLAMTLILLVLVGPLFKQRTAVYRMTTYFTLIASIFDG
                     LNACPETIKQTPIVQNLLYVAESYLPFFKLGMGWIVPAVIGFVIGLIWSFAKKEEVAD
                     "
BASE COUNT         6771 a         4762 c         4503 g         7611 t
ORIGIN      
        1 tagggggtgc gacaaaagag cttcttcaaa aaaataagtc gaaatctcac acaaattcga
       61 gaaataattt tcgtaagttt cttcttattt attgcatctg accacttctg ttacaacctc
      121 tcgttaaacg gtgttcaaaa gcttgcatct acgataataa gcccaaccca acgaaaaacg
      181 tgaggagctg ttttatttgg atgctcttat cgcttgatgc aaagacagct tttttacaac
      241 ctctacttca cggtgctcaa tcagctgacc ttcggtatta gctcaaaaaa cggtaaaata
      301 taaacagcta ttttccgttt cttcttttta atacttaggt caagatgctg ttttcacaac
      361 ctcttgttat aaataaaaaa acagttggct ggtgactgtt caaaaagcag aatccttgct
      421 tttcaaacgg tcgcagtccg ttcaactgtt tcgctgcgca ctatttatgg tttaagaaac
      481 tagctacttt tgttgtaatc aaatcaatag caacgtggtt ttctccgcct tctggaacaa
      541 tgatatccgc atagcgcttc gttggttcga taaattgatg atacatcggt ttaacaactg
      601 tcaaatattg ttcgatcacg gagtccaatg tgcgtccccg ttcttccata tcccgtttga
      661 tacgacgaat gatacgaatg tcatcatctg tatctacgta tactttgata tccatcaagt
      721 cacgcagacg ttcgtcttct aaaatcagga ttccttctag aatgatcact tcttttggtt
      781 cctgaatgat cgttgcctga cttcttgtat gcgctacata gtcatacacg ggtttttcaa
      841 tggctttata gttcagtaag tcgcctacat gctgaatcag cagatcatta tcaaaggcaa
      901 atgggtgatc gtaatttgtg ttcaatcgtt cttcaaaact caaatggctc tgatccttgt
      961 aataagaatc ttgttcaagc atcatgatgg aatggtccgg gaagttgttg aaaatcgcac
     1021 ggctgacact tgtctttccg cttccggatc cacccgtcac accaatgata attggcttgc
     1081 tttttgtcat tgactaaaac ctctttcgta tccgtttttt cattctaatc tattatagcg
     1141 aaaaaacacg aaaatgctat atttttctga aaattaatcc ttttcaattt ccaataatga
     1201 ataaagttcc tctaatgaat cgattcgata agtaggctgg atttcttcga tttctggtaa
     1261 tttatttcta ttgaaccaaa tcgtatcgat ccctgccata tttcctcctt tgatatcaga
     1321 agtcaaagaa tcaccaatga tcacagtttg ctttttatca aaattaggaa tgtgggaaaa
     1381 gacatgatca aaaaattctt tcatcggctt ttgcgctccg acagcttctg agacaaagat
     1441 atccttgaag taatcaagca ttcctgcatc tgttaatcgc cgatgttgcg tgtgcgacac
     1501 accgttcgtc acaatataaa gatcagcatg cggatggacc tttttcagga tttctagact
     1561 tcctggaaca agatcatgac cttggttcaa gtagtggcgg tagcgttttt ccatttgatc
     1621 actatctact gttttcccaa attgctggaa aagtaaccca aaacgactgc ctattatttc
     1681 ttctcgtgat agtctgcctt tttcaaactc ctgccataaa tgatgattca aagccttata
     1741 gctggtttcg atttctggtg tcaatgtgat tccttgttct gcaaacaaag cacttaaagc
     1801 attggcttca gttaattgaa aatctaacaa tgtatcatct acatcaaaca ataaagtttt
     1861 atatttcatc tttttgacct cctatttaaa ataaatactt aactttttca tctctgctaa
     1921 tgtcaacgat cgatactcgc ctactcctag ctgttcatct aattccaatg gtcccatgct
     1981 tagccgtttc aatgcggtca ctttctttcc ttgggctaag aacatctttt tgacttgatg
     2041 gaattttcct tcttggatcg tcaataaaac cgtactttgg tgcttgtctg ctttgagtat
     2101 ttttaaatca gccggcttac attgataccc atcagcgaat acgattccat tttcaaatgc
     2161 tgttacgtct gccggtgtca ccctttcgtt cacaactact tgataagtct ttgaaacctt
     2221 tttttctgga agtagcattt catatcccaa ttgtccattc gaagtgatca gcaatagtcc
     2281 tgttgtatct ctatctagtc ttcccactgg atataatggt tggaaacgat cggcacttgc
     2341 aatcagatcg attactgtcg tcttttctgg atctgataca gctgtaacag ctttttcagg
     2401 tttattgaga agataatagg actcatttgt ttctaagtgt tcacctgcta cttcgatttg
     2461 atgcagtaaa ctatctacat ttcgattttg tcgaaattcg actgtgccat ctatcatcac
     2521 ttgcttcaaa gcaaacagcc gcttcatttc cttgcgtgtc gtttctaatt tttcttctat
     2581 taatttatct aaacgcatgc tgtttccttt cttattacta ctaattaatt ataccttgaa
     2641 tattcttgct tgcaaataca tcatcatagt tattccttga cttaattaac agttcacgtt
     2701 acggttaaat agtaaagtga attggatttc tcatacacag cgtaatgacc tggtggagaa
     2761 aagttgaatc ctgactttct aaggggagat gcgcgcatct ctccttttgc gcttttttaa
     2821 gcgccctgcc ttcgatgatg tgcacgagat tagcctttag aattcactta aataaaatct
     2881 gtccgttacc aaagcagaca gtcacatttt aaggaggaat tttggttatg tcagtatcat
     2941 tagaagtaag taaaagagaa gtacgtcccc gttcattaag aaacaaactt cgtcacgaag
     3001 ggaaagttcc agctgttgtt tatgggtata aagtagaaag tacccctatc tcattcgatg
     3061 gtttgacact aagcaaattg ttgcgtgaac atggagcgaa cacagtcatc acgatggaca
     3121 tcgatggaca aaaagtcaat acattggttc acaagatcca aacgaacact tttaccaatc
     3181 atttcgaaca tgcagaattt ctatctgtaa acatgtcgga agaaacagaa gtcgaaacag
     3241 aagtcgttct tgttggcgaa tcagtgggtg ctaaatctgg tggagtgtta gctcaaaact
     3301 tgtatacagt agtcgtatct gctacaccag acaaattgcc tgaacgaatt gaagttgacg
     3361 tgacaaactt ggaattaggt gattctatta cagtagccga tcttccaaaa aacgacgatt
     3421 acaaattcgt gaccgacagt gaagagcaaa tcgctgctgt agtcgctcaa agtgaagaag
     3481 aagaaagcac aggtgaaggt ggcgaaccag aagtgatcgg ttcaacagaa gaataatact
     3541 gaaacaatca ggtggtgtta gaagcggcgg gtcagcagct tttaacacgc cttttttatt
     3601 tctaatcgtt tattttcata tggggcttcc acttttgtaa atggctttac aaacgaaaaa
     3661 tcctaattta ccaagctttt cctcgcatac ttttatcgta cctttataaa aatttatact
     3721 ttgaaaaaaa gtattcaatt attgttgcat ttttcctgat tctttattag aatgaaccct
     3781 cgttgaagta agtatttcta gtttatccta ggattaatta taaaaataaa acattcgttg
     3841 attcgaagga ggttaaacag atgtttgaaa gttttgatag cagccgcaac cgttacgctt
     3901 cagttggtgt cgtatcaagt ttgccagatg aattgattga cagtatatgg tttattattg
     3961 acctagatct taagggagtc attccattag aaaatatatt aacctttgat ttgatcgaaa
     4021 atcgtggaaa agtgacgatg catttttctc aagcaggaag caatgtggaa atggatattg
     4081 atttgccgtt tgcctattcg aaccaatttc ctgctcaagt atacgcctat gatgacggta
     4141 cgagagaaac gatcctttta ccaagtgaga ttagacaata ttaaggtgat agataaacgt
     4201 ggatgtccct tattttacag accaggccaa gttgttgatt ctttgactca attcttggct
     4261 tctttttatg gagtttcata aattatcatt gatttctaat tggtattctc aggataaacg
     4321 ctatatacta ctaactaaat tttaaagata taaggagttt aaaagatgcg cgatttaaca
     4381 aaaggaagcc ccgctaaact gattttgatg ttcactgttc ctttactgat cggaaatgtt
     4441 tttcagcagt tctacaatat gatcgatatg atcatcgtgg gtcagacgct aggtaaaaat
     4501 gcgctggcag cggttggtgc aactggaagc ctgacatttt tgatcattgg ctttgcccaa
     4561 ggtttaacag ctggtttagc cattattacc gcacaacgtt acggtgcaaa agattatcgg
     4621 ggtctgaaga aaagttttgc tgctagtgtt gtcattagtc ttgtcgtcac tgtgatttta
     4681 actgtcctaa gtctcctgtt tattcgtccg atgcttcaat tgatgcaaac tcctcctgaa
     4741 atacttgatc aagcacaaac atttatttca attattttat tagggatctt tgcatcagtc
     4801 agtttcaatt tgctttctaa tgtcattcgc gcacttggtg acagccgcac accactattc
     4861 tttctgatca ttgcggtcat catcaacgtc gtacttgatt tgatctttat tatctatttt
     4921 ggcatgggtg tggaaggtgc agctattgct acagtaatcg cacaagtttc ttccagcgta
     4981 ctgtgtctag tgtatatcaa gaaaaaaatg cctttgttac agttacgcaa aaaagatttc
     5041 aaatttgaaa aagaagagtt tgctgtacac ctaaatgctg ctttgccaat ggcgtttcaa
     5101 tcttcgatca tcgctgttgg tgctatcgtg ttacaagcag cactaaatag tctagggaca
     5161 gatgtcgtag ctgctcaagc cgctgctggt cgtatcgacc aatttgcaaa tcagccgatg
     5221 atgtcatttg gtattgcaat ggcaactttc actgcacaaa actatggtgc gaaagaatat
     5281 ggccgaatct tgcaaggggt acgtcaaact ttactgatga gtgtcggatt tagtctctta
     5341 gcaggtgcta tcgtcatttt cttcggtcat ttctttgttc aattgtttgt tagcccatca
     5401 gaaacacgtg tatttgaatt agcacaaacc tacttcaata tcaatgggtc tctttactgg
     5461 atcttagcag tcctgttcat tttacgctac acacttcaag ggcttggaca aagcaagatc
     5521 cctacgatcg ccggtatgat ggaattgctt atgcgttctt ttgcagctat cgttcttaca
     5581 ggaatgtggg gatacccagg agcagcagca gcttctcctt tagcttggat cggttctgtt
     5641 gccgtgttgc tttactctta tatgcgttcg atgaaacaat tgaaacacat gggtgaagag
     5701 gaacatttca cattagaaca tgcagaagca agatagcctt gattaaaaga aatcgttgcg
     5761 gctaaaaatg aaaactttta ttgaaaatat cttcttcgtt taccgaaaac aggtttttta
     5821 gataacaaaa aggctgtgac aaaaaatgtc acagcctttt ttcttttatt ctgtttttct
     5881 ttccgtggac tctactttga aataatcgat atgtgcatgc tggtattcta ataccgtgaa
     5941 acggaatgat gcaatctcaa tgattgttcc tgcttttatt tctttatatc ggctagaagc
     6001 aaagcccgca agtgtagtaa aattcgtttc ttcaaactct ggaatctcta catgaaaata
     6061 acgctggaaa tcatctaatg ttagtttgcc ggagatgata taagagccat ctgactgttc
     6121 cgctatcaat gcttcgtcca aatctgtctc atctcggatt tccccgacga tttcttctaa
     6181 aatatcttca atcgtcacta agccgcttgt ccctccgtat tcatctttta agatagcaat
     6241 atgcttaccc ttttttcgca tgatcgtcaa taattttttt atagggatcg tttcagttgt
     6301 tacgatcggc tcttcaatca actgttctac tgtcagctgg tcatctttat aagattgctt
     6361 gatcaaatct ggcaaggtga cataacctaa aacattgtct tttgattcta agataaccgg
     6421 atagcgagta tgtccttgtt caatcgaagc ttttatcgct tctttgacgg tttctgaagc
     6481 ttcgaatact tgcatggacg tacggtctac ttgtatatct tttgccaagg tatcatcaaa
     6541 ttcaaacacg ttttccaaat aataatattc ttctttggtc aactctcctt tttcataaga
     6601 attcaatgcg actaatgtca gctcttcttg agacaatgtc tcttctcctt cagatacgag
     6661 acgcacacct aatagcttcc ctagccctgc agctgaatga ttcaataccc aaataaaagg
     6721 aaacattact ttataaaaat aatgaagtgg tttaactacg aacaagacga ccttctccgt
     6781 attgacgata ctatagctct taggaagcag ttcgccaata accacatcga tatacgtaac
     6841 taaaataaat cccagcgcca cagagattgt actgctaata gccgaactaa aaggtagttt
     6901 accgatcact ggttctagta aagccgcgac agatccttca ccgacccacc cgatgatcaa
     6961 accggttagc gtaattccta attgactagc tgataaatag ttatctaaat ggtgtgtaac
     7021 atgaagagct agcttggcgt tctttacatt ttccttttct agttgttcca atcttgatcg
     7081 tctgattttc actaaagcaa attcacttgc tacaaaaaat gaagtaattg caatcatcaa
     7141 aaataaaata atgaaattga taaccatatt ctcccgcctt tctctttttc tattgtaccc
     7201 tttttctaca aaaagattga aaataatgcg tattcatatt taggaataaa aaaggagatg
     7261 cggcattttt cagccacatc tccttatcac ttcaaaatca ttgcgttact tgttgctaaa
     7321 cagctttttc atgacctctg ataaaaacgg tattcaaaag cttgcatcta cggcattaag
     7381 cccaaacaga tgaaaaacat gagaagctgt ttttatctgc tcgttcttac tgcttgttgc
     7441 agtacagctt tttcatgacc tctgataaac ggtgttcaag tagctgacat tcgacattag
     7501 ttcaggtcaa agcgctactt tcacaaccgc tctttcaacc atgttttccg agtatttcca
     7561 atgataagcg gaacagtttc atgttttact tcgctgtact ctagcccaaa ccatgtttct
     7621 ggcgaggtag cgattttttt gattgccagt tttgcctgca tgatttgacg ttgccatttt
     7681 cctagagttg tcacattgga aactacttca tcaatcagga caaattggaa atcgccaact
     7741 tctcgccctg gtgtcagaga gtatctttgt ggctgttttg gcaaacggcc ttgtttcatc
     7801 aaatcatgga tgacttggcg aacatatacg ttgatttcct gttggaccct gaaacctaag
     7861 aacagattca cttggacaat aaaatcagtg tccatcatat ctacttgata ttctttcgta
     7921 tatggctcat ccgtcacttc cacatgaaca aaccaataga ctcgtgctct tttaggccgt
     7981 ttatctaaaa tcgaatagat gaactgtcgg ccaactttat cttcagccat atcaggaacc
     8041 aagaagacca catttgtctg atagattggt aaagaagtat catcacgtaa ttctctaagc
     8101 tgcgggatat aatctctcaa agacacatcc tcggttgacg attcgcgaat aatgttccct
     8161 tgttcccaaa taaacatcac gactaagatg atcaaaccaa tcagaacggc tacaaaaccg
     8221 cctcggaaga atttgacaac acttgagata aagaaaacga cttctatcgc accgaagaaa
     8281 aaggtaacta aatatgcaag ccaacgagaa tgcccatttt ggataagata aaagtaaagc
     8341 agtgctgttg tcatcaacat cgtcacagtg atcgatagac cgtaagcagc ttccatatga
     8401 tgagacgttc ggaatgtgac aaccactaga gaacaggcga tccataaaat cagattgact
     8461 gctgggatat acatttgccc gatactgttc ccaggataca tgatctttaa ccgaggcagc
     8521 agtttcaact tgattgcttc tgaaactagc gtaaacgaac ctgtaatcaa tgcttgcgaa
     8581 gcaatgactg cagcgactgt tgcaaaagca acgccgatca cgatccaact ttgaggcatc
     8641 atttggaaga atggattcag gttcttgatt tgctgtgctt cagggttttg ataaacattc
     8701 aacaaccacg cagcctgccc aaaataattg ataaccaaac aaattttgac gtaaggccag
     8761 ctcagacgaa tatttcgttt gccgacatgt ccaagatcgg aataaagcgc ctctgcacct
     8821 gtcgtagcca ggaaaatatt tccaagaatg aacaagccta atttgttatc tgcactaaaa
     8881 agcagctgga tagcataata aggattcagt gcacggataa ctgtccagtc ttgactgaag
     8941 ttcattaatc ccattacgcc taaaaaagta aaccaagcga acatgattgg tccgaatgct
     9001 tttccgacaa tatctgtacc aaagcgttgg accatgaata aaacaaaaat aataactaaa
     9061 gtgataatga cgatcacgtt ctggttatta ccgaaccggt caaaaaactg cggaattcct
     9121 cgcaagcctt caatcgcagt cgttaccgtc acagcagggg tcaatacccc atctgctagt
     9181 aaggcagctc ctccgatcat tgcaggaatg atcaaatatt tcccaccttt tcggacaagt
     9241 gtgtagagtg agaaaattcc accttcacca tggttatctg cattcaatgc aatcacgaca
     9301 tatttgattg ttgtcagtag agtcaatgtc caaaaaatca atgagactga tccgataaca
     9361 aaatcaggat tgattcctga caatccgcca ttatcttcaa caatcgattt cataacgtaa
     9421 agcggacttg tcccgatgtc tccgtagacg actcccatcg ctacaagcag gccagtcatt
     9481 gatatctttt tggtattgtg cgttgttgcc acgtttttcc ctcatttctt gttatatgga
     9541 tatcctttag gtaagcttga atgatagatg atattcttat ccatgacacc cgctttccta
     9601 aattgcttct aattatccaa gtaacacact gaagtgtcaa cttatttgaa taaaattatt
     9661 ctttctttgg acgttctctg atacgtcttg ccacaaatga ataaagagca aggcaaaaaa
     9721 ttgcacccaa taatgctggt atcaaataaa aaccgccgag ttccggtccc agtggtccta
     9781 gtaaaaattc gccgagccaa ctacctaaaa aaccgataat gatatttcca gatacgcctc
     9841 ccgggacttc ctttcctaaa attgcgccag ctatcaaacc taatataccg ccgataaata
     9901 acgaccataa aaaattcagc atctcctttc cctcctttat ccatatttct cacttttatt
     9961 ttacgcttta ttcagaagga ctccaaaaat atgcgccaag aaactccaga aaaaaagaag
    10021 ttgtaacaaa agtgttcagc ttatttccgt agaagctgct tctggaacac cgtgaatcag
    10081 aggttgtgaa atcagcggtt tgacctgagt attagagaaa caaacggaaa aatagttcct
    10141 catattttgc cgttttttga gctaatatcg agggacagct gattgaacat cgtttattgg
    10201 agaggttgta aaaaagctgt tctgcatcaa gtaataagaa cagccaaata aaaacagctc
    10261 ctaatgtttt taacttggct ggacttatta ccgcagatgc aagcttttga acaccgttta
    10321 ttcgaaaaaa aaggactgtg acacggcttt tgtcacagtc cccttttttc gaataattat
    10381 tgtccccctg tcaccttcac gatcacaagt acattattcc tatgaattaa attatttttc
    10441 ttcatttttc gttgaaccat cttcgttcaa cattgctttt cttgacagat ttacgcgtcc
    10501 ttgtttatcg atttcagtta ctttcacaag gatctcatca cctagtttaa caacatcttc
    10561 gactttattg acacgatcgt ttgctaattg ggaaatatgg acaagtccat cttttccttt
    10621 gatcaagttg acgaatgcac cgaatttttc gatacggaca actttcccta gataaacttc
    10681 accaactttg acttctttgg tcaaatcttc gatgatcttg atagcttttt tgatcatttc
    10741 tgcatcagct gaagcaatac ttacttttcc atcttgatcg atatcgattt tcacaccagt
    10801 ttcatcgatg attccgttga tcgtatcgcc gcctttaccg ataacatctt tgattttagc
    10861 aggttcgatc tggatcattt cgatttttgg tgcatattgg cttaattctt cacgtggagc
    10921 agcaatcgtt gaagtcaatt cttctaagat ttccatacgt gcttgttttg cttgagttaa
    10981 tgcttcagtt aggatttgtt ctgtgattcc ttgaatcttg atatccatct gtaaagcagt
    11041 gataccatct tttgtaccgg caactttaaa gtccatatcg ccaagatgat cttccagccc
    11101 ttggatatca gttaggatcg tatagttttc accatcagaa acaagtccca tcgcaatacc
    11161 ggcgactggc gctttgattg gtacacctgc atccatcaat gctaatgtgc cagcacagat
    11221 acttgcttgg gaagaagaac catttgattc caatacttct gctactaaac ggatcgtata
    11281 aggaaattct tcttctgaag ggattacttg tgctaacgca cgttcaccta atgcgccatg
    11341 tccgatttcc cgacgccctg gagagcctgc acgtcctgta gaaccaacag aaaattgagg
    11401 gaaattgtag tggtggatga atcgtttgct gacttccacc cctaaaccat caatgatttg
    11461 gtgctctcct agaggtgcga gtgtacaagc tgataaagct tgtgtttgtc ctcgtgtgaa
    11521 caaacctgat ccatgtacac gtggcaataa gctgacctct gatgacaatg gacggatttc
    11581 atccaattta cgcccgtcag gacggatctt atcgatggtg atcaattcac gtacgacatc
    11641 tttttcaaga tcttctgtga tttgtttaac ttctttcaac aagtgatctt catcttcatg
    11701 acctgcaaat ttctcagcat aagcttcgcg gatatcgatc ttcacctgct cgatattatc
    11761 ttcgcgagca agtttttctt cagtcattac tgctgctttc attgtagcgt aagaagcatc
    11821 aaagatttct tttttcaata cttcatcaac ttgcagtaaa gtcacttcca ttttttcttt
    11881 ccctactgct tgaacgattt cttcttggaa agcaactaac tctttgatag cgtcaaaacc
    11941 aaacagcaat gcaccaagca tgtcttcttc tgaaacttct ttcgcgccgc tttcaaccat
    12001 attgatggct tgtttcgttc cggcaacagt caattcgata tctgtttgtt ctgcttgttc
    12061 tactgttgga ttcaagacgt actcgccatt tacacgacca acttctacgc ctgcgattgg
    12121 gccgtcaaat gggatatctg agattgatag tgctaaagaa gaacctagca ttgctgccat
    12181 agctggcgag cagtctgttt ctacactcat cacgatgttc gttacttgga cttcattacg
    12241 gaagccttct gcaaacatcg ggcgaattgg acggtcgatc aaacgggcag tcaatgttgc
    12301 ttccgtactt ggacggcctt ctcgtttgat gaaacctcca gggattttcc ctgctgcata
    12361 cattttttct tcataattga tcgttaatgg gaaaaagtct gtatcttttg cttctttaga
    12421 ggccacagct gcacttaaga ctactgtatc tccataacgg actaaaacgg ctccgtttgc
    12481 ttgttttgct agctgaccga cttcgatttg taagggacgg ccgccccatg tcgttttgaa
    12541 tacttgtttt tctgacataa cttctccttt tccttctcgt gaaagcaaga cgctactaaa
    12601 caaatgaaaa ctgccgaaag ccgtcttttg agcagctttc atttggttag taggagtcca
    12661 tctagcttga tcacggaatt ttacttaatt gatatacgag taaaaaaagt gagattcatg
    12721 tgaatcccac tctttttgac taaaattaac gacgcaagcc taaacgtttg atcaattcgc
    12781 ggtaacgttg aacgtcagtt ttacgtaagt aagctaacaa gttacggcgg tgaccaactt
    12841 ttttcattag gccgcggtat gaatgatggt cttttttgtg cacgcgtgca tgttcattca
    12901 agtggttgat ttcttcagtt aatacagcga tttgtacttc tggagaacca gtatcccctt
    12961 catggcgagc gtattcgttg atgatttcat tcttgcgttc ttttgaaatt gccatttctt
    13021 tcacctcttt cgattattcc cctgcgactg agtaaaacgt tggtgattcg cttaaccaag
    13081 tggcaggtca tgtgcctaga gcacacagct atactttacc tgaaaccggg ttgaaaatca
    13141 agtagttaat cattaagaaa aggtagtttt caaacaatct tactactact tttccatttt
    13201 tgtggtaaac tataatgcga aaatagactt gaaacacagt cttttaagaa aggggtacat
    13261 attccatgat tacaatggat gatatcattc gtgaaggaaa tccgacctta cgcgaagttg
    13321 caaaagaagt ttctcttcca ttaagcgaag aagatatctc tttaggaaaa gagatgcttg
    13381 agttcttaaa aaacagccaa gatccaatca aagctgaaga actacactta cgaggcggtg
    13441 ttggcttagc tgctcctcaa ttagatattt ctaaaagaat cattgctgtt cacgtaccaa
    13501 gtcctgaccc agaggcagat ggaccttctc taagtactgt tatgtataat cctaaaattt
    13561 tgagccattc tgttcaagat gcttgtttag gggaaggtga aggctgttta tctgttgatc
    13621 gagaagtacc aggatatgtt gttcgccatg ccaaaatcac tgtgtcttat tatgatatga
    13681 atggtgaaaa acacaagatt cgtttgaaga attatgaatc gatcgtcgtt caacatgaaa
    13741 tcgaccatat caacggtgtg atgttctatg accacatcaa cgatcaaaat ccatttgcac
    13801 taaaagaagg cgtattggtg atcgagtaga gaattggcag gttccttcga tactatgcta
    13861 caagaaagct ttataaaaaa ggggagtgct caaaagctgc acttcccttt tttatgtttc
    13921 tccactaatc gaaatttaga tagacatcta gaaatcgcaa ttccaaaaaa ttctgtgtat
    13981 tggatacgca tcttttctta ttttttctta tttcttccat atgggtgttt atctcagaga
    14041 tgatagaata gtttctagga taaaaagtag cagctacgat gttatccttc gtagaggatt
    14101 cctgcttcag ctagtttttg tttgatgttt ttcagcatat gcttttctgg gtagcggatc
    14161 gtatgcagat ggatgccgcc tgttaattcg gataacaaag tagcattact tttttgataa
    14221 cgtctcataa aggaatcgat atcttgtggc gttttgatgt gcaaagttcc tgctaactca
    14281 ccgtaaaggg gatgttccac aattacatca atgatttcac caccattttc aacgattagt
    14341 ctcagttctt cttctgtttg atcttttcca tgctggacag cgattttact gatgtgccca
    14401 gtattggttt cttctgtttc gaggatataa cctcttgctg ttgccacgat acttttacct
    14461 gcagctcgta gtaaagcaat atcgcctaca atgatctgac ggctgactcc aaaagttttc
    14521 gcaaacttgc tagcactcac tggtttttct gtatttgcca aggtttgcat gatttgtttt
    14581 cgccgtgtct ctccttcgat cacacttatt cctccttcat tttgttttgg tattcgcgcg
    14641 agtgtttcat caaatcaaca tacgtatctg ttattgtttc cttatatccc ttatttttca
    14701 gcagactaac aactttatcc aatacttgct gctctctttt tgcatcataa actgcttggt
    14761 tttcttcttt tttcacttgt cctattttag ttaccgtatc cattcttttt tctaataaag
    14821 caatcaactc tcgatcgatc atatcgatct tatgacgtaa tgtttcaaga gactcttctg
    14881 ttagttcttc ctctactgtc ttgatgatac gagcaggatt tcctgcaatc actacattgt
    14941 cgggaaaaga tttggtcaca accgaacctg cgccgactac tacgttgtcc cctagagtga
    15001 cacccggcgt gacgataact cctccgccta accaaacatt attaccgatt ttaatgggtt
    15061 ttgcgtactc cagaccgctg ttacgtttca ctggatgtag tggatgcgtc gccgtgtaaa
    15121 gctggacatt cggagcgaac atgcagttat caccgatttc aatcgtgctg acatctaaaa
    15181 atgtacaatt gaaatttgca taaaaattct ccccaacaaa tatattatat ccgtaatcaa
    15241 aattgataac tggctccata tagatttttt tccctgtacg gccaaaagtc tctttcaaca
    15301 attgggatcg cagttccgct gattctgctt ggttgatgat ttggctttga cttcttgcaa
    15361 acatacggtc ggcaaccagc tcaggatcat tggcaaaata aagctcgcct gcaatcattt
    15421 tttctttttc ggttttgaat tcagtcataa tctgctccta attcttgtat ttttttgaaa
    15481 acaaccgctc cgtcttcttg aataaacgta taaggctccg ctaaataaac aggcgtttga
    15541 ttgattacta agattttggc tgacggctgg cgaaactgta ccagatcaca aaacggatga
    15601 acttgaaaac tcgttccaac aatgacgatc agctctgctt ggctgacggc ataagccgcc
    15661 tgctccatga ctccatcttg aaacccttca ccatataaca cgatgtctgg gcgtatttgt
    15721 cctccgcatg cttgatgttt gtcgctgtga agatattctt tccagtctac cgtttgttga
    15781 cattttcgac aatgacattg atacaaactt ccatgaaaat tgacgagttg cgggctttta
    15841 gcctttgcgt gcaaaccgtc gatgttttgg gaaacaaccc aaacattttt ttctttcgct
    15901 agatgtgcca tttccaaatg aatgatattg ggttttgctt caggatgata aatctttttg
    15961 acaaattgat agaatttatc cggttcattc accattgctt gatgactcag caagtattcc
    16021 ggctgctcca tcccgtggta gattcctgac aaagatcgat agtctggcac tccagaaggt
    16081 gtggataccc ctgcaccggt caaaaatgta atttgctgat aagctaatat ctcttttgct
    16141 gcttcgtcta ctgttaattg tttcatgttt tgtcacctca ctttccatta tagcccatac
    16201 tcgaacaaat ttattgttga aaagtcagaa aaatgggaat aaaaaagtgt tatgggcatt
    16261 tgaacaattt atcacaatgt tcactgacaa aagaatgaaa acgtgttaca atactgctgt
    16321 ataaaagtga ggtgaatcga tatgttacca ttctttattt attggggcgt tattctggct
    16381 tgtatcgcct ggttagcgtt aagcgtttat ttctctgttt tttatcttgt acgcaaagaa
    16441 aatggaaatc tttgggcttt tgctttattc aacgtgattg ccgcaattgt tttagccatt
    16501 acattagccg tatatcgaac atggggctgg ggaatcaccc aatactcaag tttgatttat
    16561 ctaatattag ccatttatgg cgtcgttgtt cttttgcaag cgatcctagg acgtgagcca
    16621 aagaaagcgg cagcttaaat gatcgagatg gaactggaca caggaaattt tctgttctag
    16681 ttcttttttt attgtattac atacattttt gtgtataatt atactatcgt ttgcaatgga
    16741 tagatgattc acattttgat tgttttttcg gtaaaagtaa agatttctat atttatcagt
    16801 attttttcaa aaaacagcag ttatagattg acaaagcctc tcgagtatcg taaacttttt
    16861 ttgtataaat aactgaatac gagatgcctc aatcaatgag gtagaggtcg cgagaatcat
    16921 taaccctgtg gagtgaagca aacatggaga agcaggaata gggaggatcg ccgaaatgtg
    16981 cacttttttg cttggagtgt atgttgggac gcaagttaag agcttgcgga ctgtcttagc
    17041 agtgatgcta agttgcgcta tgttttgtga gaggtttatc accttatgga attttagcgg
    17101 cctttttata tctataaatc ggtcgctttt tattttttca gataaagctt taccgcattt
    17161 catctagacc gctcgtgtat catataatca gaaagggaga aaaaaatgaa aaaaaatatg
    17221 gtttcagttg ccgtactatt tgcttttgtc ttctcactat tcagtgtggg atctctggca
    17281 aatgcagatg aaaaaacacc gagcgatgag tttcgcgtag gtatggaggc tgggtatgca
    17341 cccttcaact ggtctcaaca aacagatgag aataatgcct tgccaattca aggccaaaat
    17401 agttacgccg gcggttatga tgttcaaatc gctaaaaaag tagcagatgg tttagggaaa
    17461 aaattagtca tcgtccaaac gaaatgggat ggtctggctc ctgcccttca atcaggcaag
    17521 atcgacgcga tcatcgccgg aatgagccct actgctgaac gacgaaaaga aattgatttt
    17581 accgatcctt attacgaatc acaattagta gtagtagttc aaaaagatag caaatatgca
    17641 aaagcaaaaa gtttagcaga tctttctgga gcaaagatta ctgctcagtt aaatacattc
    17701 cactatactg tgatcgatca gataccagat gtgcagaaac aacaagcaat ggataatttc
    17761 tcagcaatga gaacagcttt agcttctgga atgatcgatg gttatgtcag cgaacgtccg
    17821 gaaggtgtaa cggcaactag tgtgaacaaa gacttggaaa tgcttgaatt cagcggtgca
    17881 gatggcttcc agacaaaccc ggaagatgtt cagatttctg ttggtatgcg taaaggcgac
    17941 ccggatatcc aaaaagtaaa ccagattctt tctggcatct ctaacgatga acgaaccaaa
    18001 attatggacc aagcagtcaa agatcagcct gctgcaactg atagtgaaga aaaacctggc
    18061 atcatttctg atttcactac gatttggaaa caatacggca atatgttctt acgaggtgct
    18121 ggacttacct tgttcatcgc attgatcggg acagttgtcg gtactacact tggattactg
    18181 attggtgtct tccgttcggt acctgaatca ggaaatgcac tgactcgctt cttccaaaaa
    18241 atcggaaatg cacttctttc tatctatgtc gaagtattcc gcggaacacc gatgatggta
    18301 caagcaatgg tcatcttcta cggtctggca ctcgcttttg gcatttcatt gaaccgaacg
    18361 attgccgctt tgtttattgt ctctctcaat actggagctt acatgacaga gatcgttcgt
    18421 ggggggattt ttgcagtaga tgaagggcag ttcgaagctg cgcaagccat tggtatgacc
    18481 catggccaaa cgatgagaaa agttgttatt ccacaagttt tacggaatat cttgccagca
    18541 acaggaaatg agtttgttat caatatcaaa gatacagcag ttttaagtgt aatcggcgta
    18601 gctgatttgt tcttccaagg aaactctgct tccggtgcga atttccaatt cttccaaaca
    18661 tttacgatcg tcgggatcat ctacttagtc atgaccttta cgatcaccag aatcctgcgg
    18721 gcggtagaaa agaaaatcga tggtccttca gcttatacca agcttgaaga aatcgacaat
    18781 tcaaatttgc aggaataagg aggaagaatc atgaatgaaa aaattattga aatcaaacat
    18841 ctaagaaaaa gcttcggtga aaatgaagta ttgaaagata tcagcgtaac agtcaataaa
    18901 ggtgaagttg tgacgatcat cggttcttcc ggttctggta aatctacttt cttgcgctgt
    18961 atcaatttac tggaaaaacc aacaggcggc gagatcattt ataatggaga aaatgtatta
    19021 gctcctaaat ataatttacc taaatatcga acgaatctag gtatggtttt ccaatccttc
    19081 aacctgttca acaatatgaa tgtcttggaa aactgtatgt ctggtcaaac aactgtcctg
    19141 aaacgagata aagaaactgc caaaaaagta gcaatcgaaa atctggaaaa agttggtatg
    19201 gatcgttata tcgaagccaa accagcacaa ttatctggtg gacagaaaca gcgtgtcgca
    19261 attgctcggg cattatcaat gaatccagat gtattgctat tcgacgaacc tacttcggcg
    19321 ttagaccctg aaatggttgg cgaagtgctt aatacgatca aagacttagc acatactggc
    19381 ttgacaatgc ttatcgtgac gcatgagatg gaatttgcaa aagatgtatc tgaccgtgtc
    19441 atttttatgg ataaaggtgt gattgcagaa gaaggtacac cagaagatat ctttgtccat
    19501 ccgaaagaag aacgtacaaa agaattcctt tcacgtattt taaatgctta ggatcaatag
    19561 ctgattggga caaagagctg cggtgtttac cgcagttctt ttttatgaat tactcataaa
    19621 aggtaaattt ctgaaaattt tggaatctta gttgtgtttt tcagaaaact cttttaatat
    19681 ggaagtaatt aaactttttt agttaggatg atttaaaaat gaatgaagag caagaactgc
    19741 agcgtggact gaaaaataga catgtccaac tgatttctat tggcggagcg attggaacgg
    19801 ggttgttttt aggttccgga aagtctatcc agctagctgg cccttcgata ttgctcgcct
    19861 atcttattac tggatgcatc tgttttttca ttatgcgtgc gttaggcgaa cttctgttat
    19921 caaacaccaa taaccattct tttctggatt ttgttgcaga atatcttggc aaaaaagcgg
    19981 ctttcatcac tggctggacg tattggttct gctgggtatc gattgcaatg gccgatttaa
    20041 cagccatcgg aatgtatgta cgctactggg cgcctggtgt tcctcagtgg cttcctgaac
    20101 tgattgcatt aggtcttctt ctttgcttga atatggtggc tgtctcattg tttggtgagt
    20161 tagagttttg gtttgcattg atcaaagttg tagcgatcat tgcctttatc ctcgtaggag
    20221 cttatatgat cctcacccac tatacgacag atattggcac agccagtatc acgaatctat
    20281 ggtctcatgg cggattcttt ccaactggtg caaaaggatt tatcttagct ttccaaatgg
    20341 taacctttgc ttttgctggg atagaattag ttggattagt cgctggagaa acagaaaatc
    20401 ctgaaaaagt gttgccggaa gctatcaata atattcctat caggatcatc ttattttact
    20461 taggatctct ttttgtcatt atggcaatct atccatggaa cagcttgaat ccagataaca
    20521 gtccatttgt agaagtattt tcggaaattg ggatcacgat tgctgcttcc ttgatcaatt
    20581 tagtcgtatt aagtgcagca gcttctgcct gcaacagtgc aatatatagt acaggacgta
    20641 tgcttcgttc tttagcgcaa gaaggcagtg cccctaaaaa attcaaaaag ttaacaacac
    20701 atcatgtgcc agggaatgct ttgacatttt ctactatcgt cattttcatt tctgttatct
    20761 tgaattacgt gatgcctagt gaagtattca cactagtaag cagtattgcc actacttgtt
    20821 tcttgtttat ctggagtatg cttgtctaca cgcacatgaa atatcgtaaa agtatcctcg
    20881 gcaaaaaagc tcattcattt aaaatgccgt tgtacccatt tagtaattat ctggtatttc
    20941 tttacatggc ctttgtgtgt gtcgtcttgt tcctaggaaa agatacgaga atcgctttat
    21001 tactaacacc tgtgtggttc ttgcttctat tactaatcta tcatatgaaa tatgaacgta
    21061 agaagtaaga aagtagttgt gacagaagtg gtcagcttcg agaaataaga aggaatttac
    21121 gaaaattgct tcttaaattt ttgtgaaatt tcggcttatt tcttggagtt aaaagcttct
    21181 gtcccagcct cttttctatt tccccggaaa caaccgctga taataggcat ctgctttttt
    21241 ataggaaaaa atccaaatac cagatacaaa aaaacgtccg aataacccga tcaacggtac
    21301 aaaaaagaca ccggcaatca agcataagtt aatcatcatg gagataagac ttcgacggtc
    21361 ctttgacaaa actctcacga catgggaatc tggtttattt gctttcatga tttgcctcat
    21421 caagatacca aaagaaaaac tccacatcgt gtatactaag acataagcca tctcaggcca
    21481 aaaagcttca gggttacgcc cgacccaact agtaacagaa ggacataaag tcagccaaaa
    21541 gagcaaaaag atattcgacc agaccactcg gtaattaatt cgttctgcca tctgaaatat
    21601 acgatgatgg ttcacccaga tcaccgcaac aaatataaag ctgatggtat aagctaataa
    21661 tgtatttcgg atgacccata aagaatgaaa gctagatgta ttagggatct tgatctctaa
    21721 tacaagaact ctcaaaacaa tggctaccac tccatcagta aatgcttcta atctcgactt
    21781 tgtcacgatg attcctcctc agatgattca gcgcttttat tttatcactt acgttattaa
    21841 ataaccgtcg ttttatatag gaaattttct gaaaattgtt gcatattttt cgaaattttg
    21901 ttacttttaa aaaaaccgta aaggagttat gtatgacaaa aaaattatca tttaaagact
    21961 atctttttat aggctccatg cttttcggtc tattttttgg tgctggaaat cttatctttc
    22021 ccgttcattt gggtcaagaa gcaggcgctg ctaccttttg ggcgaatcta ggtttcttgg
    22081 ttaccggtat cggtcttccc ttcttagggg tgattgccat tggtgtatcg aaaagctcag
    22141 gtgtatatga attagcaaaa cggataaatc aaacatatgc gatggttttt accatcttgc
    22201 tttacttagt tatcggaccg ttttttgctt tacctcgcct tgcaaccaca tctttcgaga
    22261 ttggtttagc accatttatc gatcctagca gacagacgct atatctagca attttcagta
    22321 ttgtattttt tgtgctggct tggtggtttt caagaaaacc gacaaagata ttagattatg
    22381 ttggaaagtt cctgaatcct gcatttttgg ttttattagg tgctttgctt ctccttgctt
    22441 ttttgaatcc actaggttct gtaaatcaag cgcctattca gccaaattat caagagcatg
    22501 cttttttcac agggtttact caaggatata acacattaga tgcgcttgct gctttagcat
    22561 ttgggatcat cattgtcaca acaatccaaa acatgggtgt aacaaaacct gctgaaatcg
    22621 ctaaagacac gatcaaatct ggtgcgatca gcattatatt gatgggaatc atttatactt
    22681 tgctcgctta tttaggtgct atgagcttag gtagctttgc tctaagtgaa aatggcggga
    22741 tcacgctggc tcagattgct gatcattatt taggtactta tggcagtatc ttattagcat
    22801 ttatcgttat attagcttgc ttgaagaccg gtattggttt gattacagct tttgcagaaa
    22861 cattcacaga tttgtttccg caaagaaatt acgcattctt tgttgcactt gctagtatcc
    22921 ttccttgttt agcggcaaac gtaggcttga cgaatatcat tcaattttcg ttgcctgttt
    22981 tgatgttcat ctatccatta gcaatgacac tgattttact agtgttagtt ggtcctttgt
    23041 ttaagcagcg tacggctgtt tatcgaatga ctacttattt tacattgatt gcttcgattt
    23101 ttgatgggtt gaacgcttgc ccagaaacta tcaaacaaac acctattgtt caaaatcttt
    23161 tatatgttgc ggaaagctac ttgccgtttt tcaaattggg gatggggtgg atcgtaccag
    23221 cagttatcgg ttttgttatc ggactgattt ggagtttcgc taagaaagaa gaagtagcag
    23281 attgataaaa gaggttgtga aagagacgtt cagccttatt gccgataggc tgtctcttga
    23341 acactgttta ttgagaggtc gtgaaaaagc cgctcagttt cgagtattaa gaaatatttt
    23401 ttgaaaatgg cttctcacat ttttgcataa atttgactta ataccgagaa actggctttt
    23461 gaacaccgtt tattgagcgt ctataaactg acctgagtat tagagaaaca aaacggcaaa
    23521 gtagctccat atactttgcc gttttgtttt gaactaatac cgaagagaag ctgatcaaac
    23581 aacatttctt ttataatata gattttagac atatatatga tataaaaata gttttcgcaa
    23641 aaaaact
//
LOCUS       DAZKLM010000040        23501 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3764
            SAMEA115706913-rid22582563.denovo.040, whole genome shotgun
            sequence.
ACCESSION   DAZKLM010000040 DAZKLM010000000
VERSION     DAZKLM010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706913
            Sequence Read Archive: ERR13196647
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23501)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23501)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287823.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287823.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,004
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,849
            CDSs (with protein)               :: 2,849
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 41 of 96
            Pseudo Genes (incomplete)         :: 52 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 15 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23501
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706913-rid22582563.denovo.040"
                     /isolate="VRE3764"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            54..1715
                     /locus_tag="ACG842_001430"
     CDS             54..1715
                     /locus_tag="ACG842_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HFQ0247980.1"
                     /translation="MKKKFTFGLVAVCGLVLAGCYGGSSATSSSSAKSGSADGGGVFN
                     LVVPQEMPTADLSVATDTISFTALNNVYEGIYRLDEDSKPQPAGASELAEVSEDGLTY
                     KIKLREDAKWSNGDPVTAADYVYGWQRTVDPATASEYAYLYAPVENAEDITAGKKDKS
                     ELGIKAVGDYELEIKLTKQTPYFQYLLAFPSFFPQKQSVVEENGDAYASASDKAVYNG
                     PFTLEDFDGPGTDTEWTYKKNDHYWDKDTVKLSEIKVSVVKESSTALNLFKDGQADDV
                     ILSGELAQQNANDPAYTSVKEARTSYIEFNQREKNSPFNNVNLRKAISYSIDREALVK
                     QVLGDGSVVSTGLIPSDMVKSPTTNEDFAKEAGKLVSYDKDKAKEYWEKAKKELGVDS
                     LEFDLMASDDDSSKKVIEYVQNSIQENLDGVTVKPTPVPFSVRLDRSTSGDFDAVLGG
                     WAADYADPSSFTDLFVTGNSYNRGRWSNAEYDEAVKAASSTDAGDEQARWDDFQKAIK
                     IISDDMGVVPVYQKAEGHLVNEKIKGIVHHAAGASWDYKWTYVEE"
     gene            complement(1785..2132)
                     /locus_tag="ACG842_001431"
     CDS             complement(1785..2132)
                     /locus_tag="ACG842_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3899 domain-containing protein"
                     /protein_id="HFQ0247981.1"
                     /translation="MKKKSIPYAVAFLLILVILIKNLINHSFTLIQLSNDLFLWSLPF
                     LIIGGFLWVFSSGFFDHFQRSVHLARTRNRKKKPEFSSLSSASYGMYSFWLIIAGILI
                     ALSAIFMLFSLLG"
     gene            2216..3157
                     /gene="opp3b"
                     /locus_tag="ACG842_001432"
     CDS             2216..3157
                     /gene="opp3b"
                     /locus_tag="ACG842_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease"
                     /protein_id="HFQ0247982.1"
                     /translation="MNSYIKYVLKRVFFMIITLWLIATITFFLMQLLPGTPYTNQERL
                     SPETIEMLNKQVGLDKPVIVQYGIYLSNLLQGDFGISFQFKNQPVAHLLAGRIGPSLQ
                     LGLQAIIFGTVLGTILGTISAMKQNTWADTSSTLVAILGRSIPNFVFAVLLQYIFAIK
                     LQVLPIAKWDGFVYTILPTIALAMSPLADSARFIRTEMVEVLHSDYVELAKAKGLSRW
                     EIAFKHGLRNSLIPLMTLLGPLAVALMTGSLVVENIFAIPGIGEQFVKSITTNDYPTI
                     MAVTILYSFMLIFVILIVDLLYGLVDPRIRVSEGSRG"
     gene            3158..4201
                     /locus_tag="ACG842_001433"
     CDS             3158..4201
                     /locus_tag="ACG842_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0247983.1"
                     /translation="MEMIPKRYETVADIPADEFLPLQKNTEEEREQITAPSLNFIQDS
                     WRRLKKNKAAVISMVLLLVIILISVITIFVSPHDPTAQNVAYINLPPRIPGINIDGLN
                     GKAMVGGELVDKYAQANVPSNVNFFLGTDGLGRDVLSRLFMGMRISLLIAFIAALLDV
                     TIGVAYGLVSGLLGGRVDNVMQRFLEILSGIPNLVVMILMLVVFEPGIFSIVAAMAIT
                     NWIPMARIVRAQTMKLKDQEYVLAGLTLGESRMKIAFKHILPNISSVIIIQMMFSIPS
                     AIFFEAFLSFIGLGLTPPSASLGTMLSEGYKTFLYLPYLLWIPAVTLSVIMIGFNLLA
                     DGLRDAFDPKMKE"
     gene            4207..5277
                     /locus_tag="ACG842_001434"
     CDS             4207..5277
                     /locus_tag="ACG842_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0247984.1"
                     /translation="MTKILEVKDLEISFDTYAGKVRAIRGVNFHLNKGETLAIVGESG
                     SGKSVTTRSIMRLLSSNANIDAGQILFKGNDIVGKSEREMQKIRGKEIAMIFQDPMTS
                     LDPTMPIGKQVAESLRKHNKVSKKEGQKAALELLKLVGIPEAEKRINSYPHQFSGGQR
                     QRIVIAIALVCYPEILIADEPTTALDVTIQAQILELLKEIQTKIDTSIIFITHDLGVV
                     ANVADRVAVMYGGRIVEVGTSEEIFYNPQHPYTWGLLGSMPTLDSANDRLYAIPGSPP
                     DLLNPPKGDAFYPRNEFAMKIDAEQEPPFFELSKTHKAATWLLAPQAPKVTPPEEIQR
                     RWAIFKEKQKHTYGQGGIADAK"
     gene            5267..6220
                     /locus_tag="ACG842_001435"
     CDS             5267..6220
                     /locus_tag="ACG842_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0247985.1"
                     /translation="MPNKLLEVKGLKQYFNVGKKNEVHAVNDISFHIYEGETFGLVGE
                     SGSGKSTTGRTIIRLNEPTDGEILFDGQDITKIKDKKGLTKFRHDVQMIFQDPYASLN
                     PRMKVRDIIAEGIDVNGLAKSSEERAKKVDDLLRTVGLNPSHGTRYPHEFSGGQRQRI
                     GIARALAVKPRFIICDEPISALDVSIQAQVVNLLQDLQKEHQLTYLFIAHDLSMVKHI
                     SDRIGVMHNGLLLEMGTSDEIYNHGVHPYTESLLSAIPLPDPDHERKRRRIKYQPEPD
                     DGQVRQLREIAPEHFVYATEQEVPYYAKKLKRQKESLLVAN"
     gene            6232..7242
                     /locus_tag="ACG842_001436"
     CDS             6232..7242
                     /locus_tag="ACG842_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease TnpB family protein"
                     /protein_id="HFQ0247986.1"
                     /translation="MLKAFKFRMYPTEEQKQQLIRTFGCARFTYNHLLKKRQKSWQQT
                     GVANFSLTPATLKKEYPFLKEVDSLALANAQLNLDRAFRNYFKGRASFPKLKTKKSMW
                     QSYTTNNQTRTIYLENGYLKLPKQKELIKINQHRPVEGSIRSATISARYNEEFYVALL
                     CDVSPVKKESLAKWIGIAYHPKTLIQTSRPLEVTLPKFHQTEEKLQHAQRKLNVKVRS
                     AHHRKIRLDQASNYQKQKRKVMDLYLKQKNQREDYLEQLSGKLVKQYDYLFVESFPKE
                     EAHADFSIHDWHKLITKLRYKSQWYNKKFLLINTDGAEESNSVRKSQVVEKMGRHSVI
                     KG"
     gene            complement(7527..8348)
                     /locus_tag="ACG842_001437"
     CDS             complement(7527..8348)
                     /locus_tag="ACG842_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HFQ0247987.1"
                     /translation="MNRFASKEEEKLYYEHKASEEEFLNWYQKQERPEYDKPSLTVDI
                     VLMCYNKEEDQLKVLLIQRKGHPYRNSWALPGGFVQKDESTGESVLRETKEETGVVIS
                     KENIEQLHTFSTPNRDPRGWVVTVSYLAFIGEEPLIAGDDAKEVRWFTLERHSNKIHL
                     SSGEVAISLDLVTGESSGKDTLAFDHSQIILKAFNRVVNKMEHEPQVLQVLGKDFTIT
                     EARKVFAKFLGIDYKTIDHSNFKKAMLQYFDEIGERPVGIGRPSKIYQLKPGHHE"
     gene            complement(8445..8723)
                     /locus_tag="ACG842_001438"
     CDS             complement(8445..8723)
                     /locus_tag="ACG842_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0247988.1"
                     /translation="MQKKHLFFTLSIAFLSLAHLIFSYFYIRMYGYFNLHGHLNSFMT
                     AAWILRFIIDVYIVICGFFAIREERYKVLPFYLLFFLFNLILPFIFHI"
     gene            8991..11000
                     /gene="metG"
                     /locus_tag="ACG842_001439"
     CDS             8991..11000
                     /gene="metG"
                     /locus_tag="ACG842_001439"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HFQ0247989.1"
                     /translation="MAEKETFYITTPIYYPSGKLHIGNSYTTIACDAIARYKRLMGFD
                     VFYLTGVDEHGQKIETKAEELGVKPQEYVDKMAADVKKLWKTLDISYDKFIRTTDDYH
                     KAAVQKIFDRLLEQGDIYLGEYEGWYSVSDEEFFTETQLAEVYKDDEGNVIGGKAPSG
                     HEVELVKEESYFFRMSKYADRLLEYYEEHPEFIQPESRKNEMINNFIKPGLEDLAVSR
                     TTFTWGVPVKSDPKHVVYVWIDALSNYITALGYGSEDESLFEKYWPADVQMVGKEIVR
                     FHTIYWPIMLMALDLPLPKKVFGHGWLLMKDGKMSKSKGNVVYPEMLVERYGLDALRY
                     YLLRAVPFGSDGVFTPEDFVSRVNYDLANDLGNLLNRTIAMINKYCDGLVPDYASLVT
                     PFDSELSTTAANVVGRYHDAMEKMEFNTALAEIWVLISRANKYIDETEPWVLAKDEEK
                     KAELNSVMIHLAESLRIAAILLQPIMTETPEKIFNQLGLDSETMNLEGLHFGEFPSGT
                     KVVAKGTPIFPRLDMEEEVAFIQEKMSEGTQTNEDTVKWDPEETELVSTKEKQIKFDV
                     FEKVELKVAEVINCQKVEGADKLLQFRLDAGDSQDRQILSGIAEFYPDPSELIGKKVV
                     IVANLKPRKMRGQISQGMILSAEAPDGSLQVIEAPKSMPNGSEIA"
     gene            complement(11479..12066)
                     /locus_tag="ACG842_001440"
     CDS             complement(11479..12066)
                     /locus_tag="ACG842_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0247990.1"
                     /translation="MDFKRSYPKLADYLSNRVDFSRKEQQQSHWHWFTLILFGLMTVA
                     TLSQKLILLTLFIAIVALIKGPVMILWGMLYSFLVGLFPPIGILLSAIFFLLNIGTFT
                     KNWRMTLVGIYFYFYPFGVMALSEVMHWDNHWFIAGSLLLGLILLHVMLTKLYQHYGI
                     GRTIFWYVFSIPFALLTALLPSRLKTKIKGYHKIK"
     gene            12241..13563
                     /locus_tag="ACG842_001441"
     CDS             12241..13563
                     /locus_tag="ACG842_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFQ0247991.1"
                     /translation="MKGKLKREINLFGALATVMGTVIGAGVFFKTAAVTASTQSTSLT
                     LLAWLLGGFLTICAGLTVAELATAIPETGGAVKYIEAAYGKLPSFLLGWAQSLIYFPA
                     NIAALSIIFATQLTNLLQLSTDYLLLIAVITAVSVTGLNLLGTKVGASVQSVTLIVKL
                     IPIAVIVIWGLLTPGQGTVQLFPIEAGKDVTFVEGLSSALLATLFAYDGWLGVGAMAG
                     EMKRPEKDLPKAIILGLSFVTVVYLLINFVFLKTLPIDHLAGNLNAASEASDVIFGGI
                     GGKLVTIGILISVYGALNGYTLTGIRVPYAMALEDELPFSKQLTKLSKKFTVPYVPAV
                     FQLAVACIMMSLGSFDFLTDMLIFVMWLFSLLIFIGVFILRKKAPELPRPYKVPLYPI
                     VPIIAILGAIFILGMTMMTQTKLALIGIGVTLIGIPVYYQKKKKRSEE"
     gene            13718..14494
                     /locus_tag="ACG842_001442"
     CDS             13718..14494
                     /locus_tag="ACG842_001442"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HFQ0247992.1"
                     /translation="MIFDSHTHLNAEQFNEDIPETIQRAQEMGVTKMAVVGFDTPTIE
                     KSLQLSHDYPNIYSIIGWHPTEAGSYTKEIENKLQEQLTLPKVVALGEIGLDYHWMED
                     PKEVQDKVFRRQIAIAKEMNLPISIHTREALEDTYQILKEEDVRDIGGIMHSFSGDYE
                     WAKRFLDLGMHISFSGVVTFKKALDVQEAAANVPIERLLVETDAPYLAPVPFRGKRNE
                     PGYTRYTVEKIAELRNLPTEEVAAQTWTNAHRLFGLSEHG"
     gene            14487..15056
                     /gene="rnmV"
                     /locus_tag="ACG842_001443"
     CDS             14487..15056
                     /gene="rnmV"
                     /locus_tag="ACG842_001443"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043822 - ribonuclease M5 activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HFQ0247993.1"
                     /translation="MDKMKIQEIIVVEGKDDTRRLREVVDADTIETIGSAINEEILMQ
                     IEHAQETRGVIVFTDPDFSGEKIRKIIMEAVPQAKHAFLPRTQAKPKKKGSLGVEHAS
                     DAAILEALKKVVTPDISQTAVSEITRQDLLDYGLIAGAQAKEKREKLGDELRIGYTNG
                     KQLEKRLKMFRITPKELAQAMKIVEEQHE"
     gene            15049..15933
                     /gene="rsmA"
                     /locus_tag="ACG842_001444"
     CDS             15049..15933
                     /gene="rsmA"
                     /locus_tag="ACG842_001444"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HFQ0247994.1"
                     /translation="MSEYREIATPSRTKEILLKHGFSFKKSLGQNFLTEPNILRKIVE
                     TAQIDEHTNVIEVGPGIGALTEQLAKHAKQVVAFEIDDRLIPVLADTMQPYDNVTIVH
                     QDILKTDLSTAVRETFHEELPLKVVANLPYYITTPIMMHFLESDLVVDELVVMMQKEV
                     ADRISAEPGTKAYGSLSIAVQYYMEASLAFIVPKTVFVPQPNVDSAILKLTRRDIPAV
                     EVTDEKAFFRLTKAAFQQRRKTLWNNLQHSYGKDDQTKAWLAKSLETAGIDPKRRGET
                     LSLQEFAALSNAMSENMQ"
     gene            16384..17013
                     /locus_tag="ACG842_001445"
     CDS             16384..17013
                     /locus_tag="ACG842_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0247995.1"
                     /translation="MRKNSVRKLTISALLIGMGVIIPMVMPKIMIPPASFTLASHVPL
                     FIAMFFTPGVAVAVALGTTFGFFLTTPVIIALRALSHLVFALIGAWYLQKHPSIVLKN
                     GQFTFANWRFQGFNFVIGVIHSLAEMLVVSIFYFIGNMPETYYSQGYFYTVFILMGIG
                     GLIHSLVDYNIAYFIAGTLSKYFDIPVFTAAKKENRKESIQGYAKKTTY"
     gene            complement(17137..18246)
                     /locus_tag="ACG842_001446"
     CDS             complement(17137..18246)
                     /locus_tag="ACG842_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0247996.1"
                     /translation="MVEMALKNVYKKYDNAENYSVTDFNLSIKDREFIVFVGPSGCGK
                     STTLRMIAGLEDISEGELNIGGKVMNDVAPKDRDIAMVFQNYALYPHMTVFDNMAFGL
                     KLRKYDKADIKKRVDNAAEILGLTEYLKRKPAALSGGQRQRVALGRAIVRDAKVFLMD
                     EPLSNLDAKLRVAMRAEIAKLHQRLETTTIYVTHDQTEAMTMADRIVIMKDGFIQQIG
                     TPKEVYDTPVNMFVAGFIGSPAMNFFNVTLKNGVITDGHGLKLRIPEGKNKVLVEKGY
                     EGKEVIFGIRPEDIHSEQVALEASPEAVVKSEVVVSELLGAETMLYTKTGSTEFISKV
                     DARDFHKPGEIVDLAFNISKGHFFDKETEQVIKLP"
     gene            18455..18877
                     /gene="mgsA"
                     /locus_tag="ACG842_001447"
     CDS             18455..18877
                     /gene="mgsA"
                     /locus_tag="ACG842_001447"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008929 - methylglyoxal synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006007 - glucose catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019242 - methylglyoxal biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HFQ0247997.1"
                     /translation="MKIALIAHDRKKPMMVKLATAYKTILEKHELFATGTTGQRITEA
                     TGLPIHRFKSGPLGGDQQIGAMISEDQLDLVIFLRDPLAAQPHEPDVTALIRLSDVYE
                     IPLATNIGTAEVLLRGLEAGFLDFRRIVHEIDKHPLSF"
     regulatory      19064..19162
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01831"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="THF riboswitches regulate genes encoding folate
                     transporters and folate metabolism; They are most commonly
                     found in Clostridiales and Lactobacillales; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="tetrahydrofolate"
                     /db_xref="RFAM:RF01831"
     gene            19200..19736
                     /locus_tag="ACG842_001448"
     CDS             19200..19736
                     /locus_tag="ACG842_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HFQ0247998.1"
                     /translation="MKKHRLDARMIAIMGLLIALMVTLSRLVAIETPFIKISVTFIPQ
                     VIMGILFGPFWSGIGAVLADLVGMALFSKSAFFIGFTLNAFIEGAIYGFFFYRKEITW
                     KNAILATLSVTLIINLFLTPLWLALMYHVPLFSWVVWAPRLLKTVIWLPIQSIAIYYV
                     GRSIPYKKILRSLAIHAK"
     gene            complement(19776..21539)
                     /locus_tag="ACG842_001449"
     CDS             complement(19776..21539)
                     /locus_tag="ACG842_001449"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0247999.1"
                     /translation="MKVKKLSLQTLRRFIPYLTAYAKDIWLAVILGIINGIATVVMTL
                     QIGQSIDQMIGEGQVRFNRLFHLLILFGGIVLINVVSQWLIQVLSNRLAYLSVAKLRK
                     DAFAHLNRLPLRYYDQNSHGNIVSRFTNDMDNISIAISAAFNQLFSGVAIIILSFIFM
                     MRLSPLLTLVVLCSTPIIFLVSWIVARASQTNFDRQQRIIGDISGFVTERIGNQKIVK
                     AFQQEEASQQQFELLNADLYEKGQRAQFSSSLTNPSSRFVDHLAYLSIGLVGGLLALK
                     SGSSITVGVISSFTIYSSQFSKPFIELSGITTQLQTAFSGLERAFDLIDQPEEKPDLP
                     DAYALEPKNIKGKVSFDHVSFSYDPKQRLIEDFNFTAYPGQTIAIVGKTGAGKSTLVN
                     LLMRFYDTTKGTITVDDHDIRSIQRDTLRKSFGMVLQDTWLFDSTLRENLRFGNPEAS
                     DAEINDALKASYMYEFVERLPEKLDTKIGGQGLKISDGQRQLLTIARTMISNPPMLIL
                     DEATSSVDTLTEKKIQQAFLAMMEGKTSFVIAHRLSTIKNADQILVMDQGAIVEMGTH
                     DELLQKDGYYRRLYQAQFTAQ"
     gene            complement(21542..23257)
                     /locus_tag="ACG842_001450"
     CDS             complement(21542..23257)
                     /locus_tag="ACG842_001450"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0248000.1"
                     /translation="MIQLLRYAKDYRKQIILGPFFKFLEAVFELVLPLMMASLIDNGL
                     KMNDRGKIIEMGLWMVAMSVIGLICAIICQYYASVASQGFGTELRNQLIKKINTFSHN
                     ELNHFGTDTLITRMTNDINQLQLALAMVIRLLIRAPFLSIGSVVMAFVIDWEVGLFFL
                     ALLPIFSIILFFIIKKTVPLYQKVQEKLDQLNEHVSQNLSGVRVIRAFAQTKKETEKF
                     DKSTNDLANNYLRVSNISALLSPATTLIMNVGIICLLTVGGIKVNIGSLQQGQVLALI
                     NYMNQMLLALIVVSNLVVIFTRAEASGNRVKEVLDTENSILEAEVSTTPDFSSEAIIA
                     FDHVDFRYTPDSGLSLQNITFQLKRNTVLGIIGPTGSGKTTLTQLIPRFYDVSSGTVI
                     MDGMDVRSWQLDHLRQQIALVPQTSVLFTGTIRENLQWGKKDATEKECWEALKIAQAE
                     EFVRQLPNGLDTKVMENGKNFSGGQKQRLTIARALIAKPALLILDDSLSALDYQTDLD
                     LRKALQTYLGSTVIIISQRVRSIQEAHHILVMDQGKIAAQGTHEELLAQSAEYQEIVA
                     SQEEE"
BASE COUNT         7573 a         4303 c         4778 g         6847 t
ORIGIN      
        1 caacttcaga aaatattaat tattctgaaa attccaataa aggggtacgt aaaatgaaaa
       61 agaaatttac atttggtttg gttgcggtat gcggccttgt acttgctgga tgttatggcg
      121 gcagttcagc cacaagcagt agttcagcaa aaagcggttc agcagatgga ggaggcgtat
      181 ttaacttagt cgtgcctcaa gaaatgccta cagctgactt atcagttgca acagatacaa
      241 tcagttttac agcattgaat aacgtttatg aaggaattta ccgtcttgat gaagacagca
      301 aaccacagcc ggcaggagct agcgagttgg cagaagtcag tgaagacgga ttaacttaca
      361 aaatcaaatt acgagaagac gcaaaatggt caaatggtga tcctgttaca gcagcggatt
      421 acgtttatgg ctggcaacga acagtagacc cagctacagc ttctgaatat gcttacctgt
      481 atgcgccagt tgaaaatgca gaagacatta cagctggaaa gaaagataaa tcagaactag
      541 ggatcaaagc agttggtgac tatgaattag aaatcaaatt gacgaaacaa acaccatatt
      601 tccaatatct gcttgctttc ccatcatttt tcccacaaaa acaatccgta gtggaagaaa
      661 atggggatgc ctatgcatct gcaagtgata aagctgttta taatggacca ttcacattag
      721 aagattttga tggaccagga acagatacag aatggactta taagaaaaat gatcattact
      781 gggacaaaga tacagtaaaa ctttctgaaa tcaaagtcag tgtagtaaaa gaatcttcta
      841 ctgcattgaa cttattcaaa gatggccaag cagatgacgt gatcttgtct ggtgagttag
      901 cacagcaaaa cgcaaacgat ccagcttata cttctgttaa agaagcgcgg acaagctata
      961 tcgaattcaa tcaacgtgaa aagaattcac cattcaacaa tgtgaaccta agaaaagcta
     1021 tttcttattc tatcgatcgt gaagctttag taaaacaagt actaggtgac ggatcagttg
     1081 tctctacagg attgatccca tcagatatgg taaaaagtcc aacaacgaac gaagactttg
     1141 caaaagaagc tggaaaatta gtttcttatg acaaagataa agcaaaagaa tattgggaaa
     1201 aagcgaaaaa agaattgggc gttgattctt tagaatttga tttgatggct tctgatgatg
     1261 attcatcgaa aaaagtaatc gaatatgttc aaaactcaat ccaagaaaac ttggatggcg
     1321 taacagtcaa accaacacct gttccattct ctgtacgtct tgaccgctca acttcgggtg
     1381 attttgacgc agtactaggc ggatgggctg ctgactatgc agacccaagc agctttactg
     1441 atttgtttgt cacaggtaat tcatacaacc gtggtcgctg gagcaatgca gaatatgacg
     1501 aagcagttaa agcagcttca tcaacagacg ctggcgatga acaagcccgt tgggatgatt
     1561 tccaaaaagc aatcaagatc atctctgatg atatgggtgt cgttccggtg taccaaaaag
     1621 cagaaggtca cctagtgaat gaaaaaatca aaggtatcgt tcaccatgcg gcaggcgctt
     1681 catgggatta caaatggaca tacgttgaag aataatcgtt aataccgtga gcatgagaaa
     1741 aaacgaggct gcggcaaatg tcgcaacctc gttctcatat tcacttatcc taacaaagaa
     1801 aaaagcatga aaatagcaga aagagcaatt agtattccgg caatgatcag ccaaaagctg
     1861 tacataccgt agctggcaga cgagagcgag gaaaattctg gttttttctt tcggttcctt
     1921 gttcgtgcaa gatggaccga tcgctgaaaa tgatcgaaga aacccgaaga aaagacccat
     1981 aaaaaaccgc cgataatcag aaacggcaat gaccataaga atagatcatt agataattga
     2041 atgagtgtaa aggaatggtt gatgagattt tttattaaaa taaccagaat cagtagaaaa
     2101 gctactgcgt aggggataga tttttttttc atttgattgc tcctttattt ttggttcatt
     2161 atctaataat gtagcgaaac atctgtcaga agtcaaaagc tagggggaaa cattagtgaa
     2221 tagttatatc aaatatgtat taaaacgtgt ctttttcatg atcatcacgc tttggttaat
     2281 tgcaacaatc acctttttct tgatgcaatt gctgccgggc acgccatata cgaatcagga
     2341 acgtttgagt cctgaaacaa tcgagatgct aaataaacaa gtcggtttgg ataaaccagt
     2401 tatcgtacaa tatgggattt atctttctaa tttgcttcaa ggagattttg gaatttcctt
     2461 ccaatttaaa aaccagccag tcgcacattt gttagctgga agaatcggac catccttgca
     2521 gttagggtta caggcaatta tctttggaac agtcctaggg acgattttag gaacgatctc
     2581 tgccatgaag caaaatacat gggcagatac atcttctaca ctcgtagcga tattaggtcg
     2641 ttccattcca aactttgtat ttgctgtttt attacaatat atctttgcaa tcaaattaca
     2701 agttttgcca atcgcaaaat gggatggctt tgtgtacaca atcttaccaa caattgcgtt
     2761 ggccatgtcg ccgcttgctg actctgctcg gtttatccgt acggaaatgg tggaagtact
     2821 gcatagtgat tatgtggaat tagcaaaagc aaaaggattg agccgttggg aaatcgcctt
     2881 taaacatggt cttcgaaata gtttgattcc attgatgact ttattaggac cgttagctgt
     2941 agcattgatg accggatcac tggtggtaga aaatatcttt gctattcctg gtatcgggga
     3001 acaattcgtt aagtcaatca caacaaatga ttatcctacg atcatggctg taacgatcct
     3061 ttactcattt atgctgattt ttgttatttt gatcgtggat ctattgtacg gtctagttga
     3121 tccaagaatt cgtgtttcag aagggagccg aggataaatg gaaatgatac caaagcgtta
     3181 tgaaacagtt gcagatatcc ctgcggatga atttcttcca ttacaaaaaa ataccgaaga
     3241 agaacgtgaa cagattactg caccttcttt gaattttatc caagattcat ggcgccgttt
     3301 aaagaaaaac aaagctgccg ttatttcaat ggttttacta ttggtcatca ttttgatctc
     3361 tgtgatcaca atctttgttt ctcctcatga tccaactgcc caaaacgtgg cttatatcaa
     3421 tttgcctcct cgtattccgg gaatcaatat cgatggattg aatggaaaag caatggttgg
     3481 tggtgaactt gttgataaat atgctcaagc aaacgtccct tcaaacgtaa atttcttttt
     3541 aggaacagac ggtttgggac gtgatgtatt aagccgctta tttatgggaa tgagaatctc
     3601 gttgctgatc gcatttattg ctgcattact agatgtaacg attggtgtag cttacgggtt
     3661 ggtttctggt ttgttaggcg gacgtgtcga taacgtgatg caacgttttt tggaaatctt
     3721 atcaggaatt ccaaacctcg ttgtcatgat tttgatgttg gtcgtttttg aaccaggaat
     3781 tttttcaatc gtggctgcta tggccatcac aaactggata ccaatggcac gtatcgttcg
     3841 tgcacaaaca atgaaattaa aagatcaaga atatgtttta gctggattga cattaggtga
     3901 atcaagaatg aaaattgcat tcaaacatat tttgccgaat atctctagtg tcattattat
     3961 tcaaatgatg tttagtattc catctgctat tttcttcgaa gcctttttaa gctttatcgg
     4021 tttaggtctt acgcctccgt cagcttcatt aggaacgatg ttgagcgaag ggtacaagac
     4081 attcctgtat ctgccttatc tattatggat tccggcagta actttatcgg ttatcatgat
     4141 tggctttaac ttacttgctg atggattgcg tgatgccttt gatcctaaaa tgaaagagtg
     4201 aagacgatga cgaaaatttt agaagtgaaa gacttagaaa tctcttttga tacgtatgct
     4261 ggaaaggtac gagcgattcg cggtgtcaat tttcatttga ataaaggaga aacattggcg
     4321 atcgttgggg aatctggaag tggaaaatca gtgacgacca gaagcatcat gcgcttgctg
     4381 tcaagcaacg ccaatataga tgctggtcaa atcctcttta aaggtaacga tatcgttgga
     4441 aaatcagaaa gagagatgca aaaaatccgt ggtaaagaaa tagcgatgat tttccaagat
     4501 ccaatgacct ctttagatcc gacgatgccc attgggaagc aagtcgctga gtcccttaga
     4561 aagcataata aagtttcaaa aaaagaggga caaaaagctg cattagaatt gcttaaatta
     4621 gttggaatac ccgaagctga gaaacgtatc aacagttatc cgcatcagtt ctcaggaggt
     4681 cagcgtcaac ggatcgtgat cgcgattgca ctggtatgtt atccggaaat actgattgct
     4741 gatgaaccta caacagcgct tgatgtgact attcaagcac agattttgga gctgctgaaa
     4801 gaaattcaga caaaaatcga tacatcaatt atctttatca ctcatgacct tggtgtagta
     4861 gcgaatgtcg ctgatcgtgt cgctgtcatg tatggaggaa gaatcgttga agttggaaca
     4921 tcggaagaaa ttttctacaa cccgcaacat ccctatactt ggggactgct tggttcaatg
     4981 ccgacattag atagtgcgaa cgatcgattg tatgctattc ctggttctcc accggattta
     5041 ttgaacccgc caaaaggaga cgcattctat ccaagaaatg aattcgcgat gaaaatcgat
     5101 gcagaacaag aaccaccatt ttttgaacta tcaaaaacac acaaggcagc aacttggcta
     5161 cttgctccgc aggcacccaa agtgacaccg ccagaagaaa tccaacgtcg ctgggctatc
     5221 tttaaagaga aacaaaaaca tacttacggt caaggaggaa ttgctgatgc caaataaact
     5281 acttgaagtt aaaggattga aacagtattt caatgtagga aaaaagaatg aagtacatgc
     5341 tgtaaatgac atcagctttc acatttatga aggagaaaca ttcggtttag ttggagaatc
     5401 tggaagcgga aaatccacta ctggccgtac gatcattcgc ttgaatgaac ctactgatgg
     5461 tgagatatta ttcgacggtc aagatataac gaagatcaaa gataaaaaag gtttgacgaa
     5521 attccggcat gatgttcaga tgatctttca agatccatac gcttcgttga atcctcgtat
     5581 gaaagtcaga gatatcattg ccgaaggaat tgatgttaat ggactagcta aatcatctga
     5641 agagcgtgca aaaaaagtcg atgatctatt gagaactgtt ggattgaatc caagccacgg
     5701 tactcgttat cctcatgaat tttcaggtgg gcaacgccag cgtattggta ttgcacgagc
     5761 attggcagta aaaccgcgtt ttatcatttg tgatgaacca atttctgcac tagatgtgtc
     5821 gatccaagcc caagtggtca atttgttgca agatctccaa aaagaacatc aattgaccta
     5881 tctatttatt gcacatgatt tgtcgatggt taaacatatt agtgaccgaa tcggtgtaat
     5941 gcataacggc ttgttattag agatgggaac aagtgatgaa atctataatc acggtgttca
     6001 tccttataca gaaagtttgt tatctgctat tcctctacca gatccagacc atgagagaaa
     6061 acgcagaaga atcaaatatc aacctgaacc agatgacgga caagtacgtc aacttcgtga
     6121 aatcgcacca gaacattttg tctatgcaac tgagcaggaa gtcccatatt acgcaaaaaa
     6181 actaaaacga caaaaggaat ctcttctggt tgctaactag gagggacata gatgctaaaa
     6241 gcttttaaat tccgtatgta tccgacagaa gaacaaaaac agcaattgat tcgtactttt
     6301 ggatgtgctc gatttaccta taaccatttg ctgaaaaagc ggcagaaaag ttggcaacaa
     6361 acaggagtag ccaatttttc gctcacaccc gctacattaa aaaaagaata cccattctta
     6421 aaagaagtag acagtcttgc tttagcaaat gcacagctta atttggatcg ggcttttcgc
     6481 aattatttca agggacgtgc cagcttccca aagcttaaaa cgaaaaaaag tatgtggcaa
     6541 tcttatacga ccaacaacca aacccgtacg atctatctgg aaaatggcta cttaaagtta
     6601 ccaaaacaaa aagaactaat caagataaat cagcatcggc cagttgaagg atcgattcgc
     6661 tccgctacga tttctgctcg atataatgaa gaattttatg tcgcactact ttgtgatgtt
     6721 tcgccagtaa aaaaagaatc tttagctaaa tggatcggga tcgcctatca tccgaagaca
     6781 ttgatccaaa catctcgacc tctagaagta actttaccaa aatttcacca gacagaagaa
     6841 aagctgcaac atgcacagcg gaaattgaat gtgaaagtcc gctccgctca ccatagaaag
     6901 atcaggttag atcaggcaag caattatcag aaacaaaaga gaaaagtaat ggatctttat
     6961 ctgaaacaaa agaaccagcg agaagattat cttgaacaat tatctgggaa attggtcaaa
     7021 caatatgact acttatttgt agaatctttt ccaaaagaag aggcacatgc tgatttttct
     7081 attcacgact ggcataaatt aataacaaaa ttgcgttata agtcccaatg gtataataaa
     7141 aaatttcttt tgatcaatac cgacggagca gaagaaagca attccgtaag aaagagccaa
     7201 gtagtagaaa aaatgggcag acattcggtt atcaagggat aaaaaatgaa tgaaactgat
     7261 aggagcgtta gggttagcct tgttaataaa aagtcttaat gacttttgtt tctaggaatt
     7321 tttcttttga agaaaagctc actaattatg acaaaccaat aaaacaatag cagaaaaagt
     7381 tcgtacgagc tttttctgct attgttaatt ttttattcag aaagtttacg aatacatcaa
     7441 aattctgaaa caaaaaacgt aactcacaaa gagttgtttt tgcgtttttt cttgaatgat
     7501 taggtctgaa tcttttgctc acaaccttat tcatggtgcc ccggcttgag ctgatagatt
     7561 tttgaaggac ggccgattcc tacaggacgt tcaccgatct catcgaaata ttggagcata
     7621 gctttcttaa aattggaatg atcgatcgtt ttataatcga tccctaagaa tttagcaaat
     7681 acttttcgag cttccgtaat cgtgaaatct ttacctaaaa cttgtagaac ttgcggctcg
     7741 tgttccattt tattgactac acggttgaat gctttcaaga tgatttgact atggtcaaaa
     7801 gccaaagtat ctttgccaga agactctcca gtgactaaat caagcgagat agccacttct
     7861 ccgctagata aatggatctt gttactatga cgttctagtg tgaaccagcg tacttccttg
     7921 gcatcatctc cggcgattag cggttcttct ccaataaagg ctaagtagct gactgtgacg
     7981 acccagcctc gcggatctcg attaggcgta ctgaatgtat gcagttgctc aatattttct
     8041 tttgaaataa ccacaccagt ttcttccttt gtttctctta agacgctttc tccagttgac
     8101 tcatcctttt ggacaaagcc accaggcaat gcccatgaat ttcgataagg atgccctttt
     8161 ctttggatca acaaaacttt cagctgatct tcttctttgt tatagcacat cagtacgata
     8221 tcgacagtca aagagggctt gtcatattct ggacgttcct gtttttgata ccagttaaga
     8281 aattcttctt cacttgcttt atgttcataa tataattttt cttcttcttt agaagcaaaa
     8341 cggttcatct gaccacctcc attgacagta aaagtacttt ttaccttatt gtagcagact
     8401 ctgaaaaaaa accaaggatt tacttgaaaa atctaggtta tcatttaaat atgaaaaata
     8461 aatggaagta tgagattgaa taaaaagaaa agcagataaa acggcaatac tttataccgc
     8521 tcttcacgaa tggcaaagaa gccacaaata acgatatata cgtcaatgat aaaacgcaaa
     8581 atccaagctg ctgtcataaa actgttcaaa tgcccatgga ggttaaaata accatacata
     8641 cgaatataaa aatagctgaa tattaaatgt gctaatgaaa gaaaagcaat actaagtgta
     8701 aaaaaaagat gtttcttctg catgattgtt ccagtccctt actaagatag atagtcttta
     8761 ttataccacg tgtgagggga gaaataaaaa gccgtttttt agtagatatg aaacaaaaca
     8821 aaaacccgca tttattcaac ctattcatcc ttttaaagga tagattgaac gtagatagaa
     8881 gtcgtttatc cagtcttttt cattttgaaa aaaagctaac agcatgcttt ttttatggta
     8941 tactagcaag gaaacgaatt cgcatcatgc gataaaggag gaagagcccc gtggcagaaa
     9001 aggaaacttt ttatatcact acgccaatct attatccaag cggcaaactt catatcggca
     9061 actcgtatac tacgattgca tgtgacgcaa ttgctcgtta taaacggttg atgggctttg
     9121 atgtgtttta tctcactgga gtggatgaac atggtcaaaa aatcgaaaca aaagcagaag
     9181 aactaggtgt aaaaccgcaa gaatatgtag ataaaatggc ggctgatgtc aagaaattat
     9241 ggaaaacgct tgatatcagt tatgataaat tcattcgcac gacagatgat taccataaag
     9301 cagctgttca aaaaatattt gatcgtttat tagaacaggg agatatttac ttaggtgaat
     9361 atgaaggctg gtactctgtt tctgacgaag aattctttac agaaacacaa cttgcagaag
     9421 tgtacaaaga tgacgaaggt aacgtgatcg gtggtaaagc gccaagtgga cacgaagtag
     9481 aattggtaaa agaagaatca tacttcttcc gcatgagcaa atacgcagac cgtctattgg
     9541 aatactatga agaacatccc gaattcatcc agccagaatc tcgtaaaaac gaaatgatca
     9601 acaactttat caaaccagga ttagaggatc ttgcagtttc acgaacaact tttacttggg
     9661 gagtaccagt caagagtgat ccgaaacatg tggtgtatgt atggatcgat gcattatcca
     9721 attatattac tgcactagga tatggctccg aagatgaaag tctattcgaa aaatactggc
     9781 ctgcagatgt tcagatggtc ggtaaagaaa tcgttcgctt tcatacgatc tattggccaa
     9841 tcatgctgat ggcccttgat ttgcctctac ctaaaaaagt atttgggcat ggctggctat
     9901 tgatgaaaga cggaaaaatg tctaaatcaa aaggaaatgt cgtttatcct gaaatgcttg
     9961 tggaacgtta tggtttagat gcactccgtt attatttgct tcgtgctgtt ccgtttgggt
    10021 cagatggtgt ctttacgcca gaagattttg tttcccgtgt caattatgat ctggcaaatg
    10081 atctagggaa cttattgaat cgaacgattg ctatgatcaa caaatattgt gatggactcg
    10141 ttcctgacta tgcctcactt gtcactccat ttgatagtga actgtctacg acggctgcta
    10201 acgttgtcgg acgttaccat gatgcaatgg aaaaaatgga attcaataca gcattggctg
    10261 aaatctgggt gctgatttct cgtgcaaaca aatacattga tgaaacagaa ccatgggtat
    10321 tagcaaaaga cgaagaaaag aaagctgaat tgaatagtgt catgatccat ttagctgaat
    10381 ctttgcgtat cgcagcgata ttacttcagc caatcatgac agaaacgcca gagaagatct
    10441 tcaatcaact aggattggac tctgaaacaa tgaatcttga aggattacac tttggcgaat
    10501 ttccttcagg aacaaaagta gttgcaaaag gtacaccaat cttcccacgt ttagatatgg
    10561 aagaagaagt tgcttttatt caagaaaaaa tgtctgaagg tactcaaaca aacgaagata
    10621 cagtcaaatg ggatccagaa gaaacagaac ttgtttcaac gaaagaaaaa caaatcaaat
    10681 ttgatgtatt tgaaaaagta gaactaaaag tagcagaagt catcaactgt caaaaagtag
    10741 aaggggcaga taagttgctg caattccgac tagatgctgg agacagccaa gaccgtcaaa
    10801 tcttgtcagg aatcgctgaa ttttatcctg atccaagcga attgatcggg aaaaaagtcg
    10861 tcattgtagc aaatctgaaa ccacgaaaga tgcgtggaca aatcagtcaa ggaatgatcc
    10921 tttctgcaga agcaccagat ggctctttac aagtaatcga agcaccaaaa tccatgccaa
    10981 atggctcaga aatcgcatag aaattcaaag ggggtatgac actagttgtg tcataccccc
    11041 tttttccgaa atttgagaag caattttcgt aaattccttc ttattacttg aagccaatca
    11101 tataatacag aaacagagaa ctatccaaaa taaatcctag aaaaagaagt acgagcttcg
    11161 ctgatttttg ttaatttaga ataatactaa taggaatcgt aaaaacagaa aaaaagcata
    11221 atgataaagg gtcgggcaga ttttaattga ttcatctggt actctttttt attgtcctta
    11281 cattcataaa gtttgaatgc aatgacttta ttacatctat atttatatct aacagtagcg
    11341 ttgatgtgtt aattataagt tctatcactt tatgttcaat tagtttgtgc tcttatatgt
    11401 gtgattgaaa atatagataa aaaaacaatc atcggctttg acttcgatga ttgttttctt
    11461 tattttgtaa aaatcctatc acttgatttt atggtaacct tttatttttg tcttcaaacg
    11521 gcttggtaag agagcagtaa gcagtgcaaa tgggatcgaa aagacatacc agaaaatcgt
    11581 acgtccgata ccataatgtt gatagagctt tgtcaacatc acgtgtaaca agatcaaccc
    11641 taaaagcaag gagccagcta taaaccaatg attatcccaa tgcatgactt cactaagtgc
    11701 catcactcca aaaggataga aataaaaata gatcccgact aaggtcatcc gccagttctt
    11761 tgtaaatgtt ccgatattca ataaaaagaa aatcgctgaa agcaaaatac cgataggcgg
    11821 aaaaagccca accaaaaagg aatacaacat cccccacaag atcatcacag gacctttgat
    11881 caatgcaacg attgcgatga acaaagtcag caatattagt ttttgtgata acgttgcaac
    11941 agtcattaat ccaaataaaa ttaatgtgaa ccaatgccaa tgggactgct gttgttcttt
    12001 acgggaaaaa tcgacccggt tggataaata atctgctaat ttcggataag aacgcttaaa
    12061 atccataaac tgttcctcct tcgtggaaaa ggtaggttta gtatacgaaa aataaaaaag
    12121 aaatacatcc gtcttaagag ggagtttttc actgaaagtg gagaaaaaag aatggtaaat
    12181 taaaatattc tcagttatac tgaaaattat accaaatttc aaagaagaaa gaggatggga
    12241 atgaaaggca aactaaaacg agaaatcaat ctgtttggcg cattagctac cgttatggga
    12301 accgtgatcg gtgcaggcgt cttcttcaaa actgctgctg ttactgctag tactcagtct
    12361 acaagtttga cattgctagc ttggcttttg ggtggtttct taacgatctg tgctggattg
    12421 acagtggctg aattagctac agcaatcccg gaaactggtg gagctgtaaa atatattgaa
    12481 gctgcgtatg gaaaattgcc cagcttttta ttaggatggg cgcaaagctt gatttacttt
    12541 cctgctaata ttgctgcatt atcgattatc tttgcgacac aattgacgaa cttattacaa
    12601 ttatctaccg attatctttt gttgattgca gtgatcaccg ctgtttctgt gacagggttg
    12661 aatttgttag ggacaaaagt cggtgcttct gtacaatcag tgacgttgat tgttaaatta
    12721 ataccaattg ctgtgattgt catatgggga ttattgaccc caggacaagg aactgtccag
    12781 ttatttccaa tcgaagctgg aaaagatgtt acttttgtcg aaggattgag cagtgcgttg
    12841 ttagcaacgt tgtttgctta tgacggctgg ttaggtgtcg gtgcaatggc aggggaaatg
    12901 aaacggccgg aaaaagattt gcctaaagca attattcttg gtttgagttt tgtaacagtc
    12961 gtttatctat tgatcaattt tgtttttttg aaaactttgc cgattgatca tcttgccggt
    13021 aatttgaatg cagcttctga agcatcagac gtcatttttg gaggaatcgg tggaaagctg
    13081 gtcactattg ggatcttgat ctcggtatac ggtgcattga acggttatac actaactggg
    13141 atccgcgtgc cttatgcgat ggctttagaa gacgagctac ctttcagtaa acagctgaca
    13201 aagctttcaa agaagtttac ggttccttat gttcctgcgg ttttccagtt agcagtagct
    13261 tgtattatga tgagccttgg ttctttcgac tttttaacag acatgttgat ttttgtgatg
    13321 tggctattca gtttactgat ttttatcggt gtctttattt taagaaaaaa agcgccggaa
    13381 ttgccacgtc catataaagt accactttat ccaatcgttc cgattattgc gattctcggt
    13441 gctattttca ttttaggtat gacgatgatg acgcaaacga aattagcatt gatcggtata
    13501 ggtgttacct tgataggtat cccagtctat tatcaaaaaa agaaaaaacg ctcagaagag
    13561 taaaatattt caacaacaga cagaaaaagt acgaactagg tcctctagat cttcgtgctt
    13621 tttctatttc aataaaacaa gttcttctat tttgattttt gctacttctg caagtagtgc
    13681 ttatgtgcta aagtaagaat gaaaaggaga gataaacatg atatttgatt ctcataccca
    13741 tttaaatgct gaacagttca atgaagatat tccagaaacg attcagcgag cacaagaaat
    13801 gggggtaacg aaaatggcgg tcgtgggctt cgatacgcca acgattgaaa agtctttgca
    13861 attaagccat gactatccaa atatatacag tattatcggc tggcatccga cagaggctgg
    13921 cagttatacg aaagaaatcg aaaataaatt acaagaacaa ttgactttac caaaggttgt
    13981 agcattaggg gaaattggct tggattatca ttggatggaa gacccgaaag aagtccaaga
    14041 taaagtattt cgcagacaga ttgctatcgc aaaagaaatg aacctaccaa tcagtattca
    14101 tactagagaa gcacttgaag acacgtatca aatcctaaaa gaagaagatg ttcgagatat
    14161 cggtggaatc atgcatagtt tcagtggtga ttatgaatgg gcaaaacgat ttcttgactt
    14221 agggatgcat atttcattta gcggtgtcgt cactttcaaa aaagcattag acgtccaaga
    14281 ggctgcagca aatgtaccaa tcgaacgatt acttgtggaa acagatgccc cttatctggc
    14341 accagttcct tttagaggaa agcgaaatga accaggctat actcgttata cagtagaaaa
    14401 gattgctgaa cttcggaatc ttccgacaga agaagtggct gcccaaacat ggacaaatgc
    14461 gcaccgatta tttggtctaa gcgaacatgg ataagatgaa gatccaagag attatagtcg
    14521 ttgaaggaaa agacgataca agaaggctgc gggaagtagt tgatgcagat acgatagaaa
    14581 caatagggtc tgcaatcaat gaagagatcc tgatgcaaat tgaacatgcc caagagactc
    14641 gtggagtgat cgtttttacc gatcctgatt tttcaggaga aaaaataaga aaaatcatta
    14701 tggaagctgt tcctcaggca aaacatgctt ttttgccgcg cacacaagct aaaccaaaga
    14761 aaaagggaag ccttggtgtc gagcatgcaa gtgatgctgc tattttagaa gcattgaaaa
    14821 aggtcgtgac gccagacatt tcccaaacgg ctgtttctga aatcactaga caagaccttt
    14881 tagactatgg tttaatagct ggggcacaag caaaagaaaa acgagaaaaa ctaggtgacg
    14941 agttgcgcat cggttacacg aacggcaaac aattggaaaa acgtttgaag atgtttcgta
    15001 ttacgccgaa agaactcgcc caagcaatga aaatagtgga ggaacaacat gagtgaatat
    15061 cgtgaaattg cgacaccctc tagaacaaaa gagattttat tgaaacatgg tttttcattt
    15121 aaaaaaagtt taggacaaaa ttttctgact gaaccaaata ttttaaggaa aatcgtagaa
    15181 actgcgcaaa tagatgaaca tacgaacgtg atcgaagttg gaccaggaat cggtgcattg
    15241 acggaacaat tagccaagca tgcaaaacaa gtcgtagcat ttgagatcga tgatcgtttg
    15301 atccctgtat tggctgatac gatgcaaccc tatgacaatg tgactattgt acatcaagat
    15361 attttaaaga ccgatttgtc aacagctgtt agagaaacat ttcatgagga gcttccgtta
    15421 aaagtcgtag cgaatttacc ttactatatt acgacaccga tcatgatgca ttttcttgaa
    15481 tcagatttgg ttgtggatga actagtcgtc atgatgcaaa aagaagtagc tgatcgtatc
    15541 tctgctgaac caggaacgaa agcttatggt tcattatcga ttgctgttca atattatatg
    15601 gaagccagtt tagcattcat cgtaccaaaa acagtatttg tccctcagcc aaatgtagat
    15661 tcagcgattt tgaaactgac aagaagagat ataccggcag tcgaagtgac agacgaaaaa
    15721 gcatttttcc gattgacaaa agcggcattt cagcaaagaa gaaaaacgct ttggaataac
    15781 ttgcagcatt cgtatggcaa agatgaccag acaaaagcat ggttagcaaa aagcttggaa
    15841 acagctggaa tcgatccaaa acgtcgtggg gagactttgt cattacaaga attcgccgca
    15901 ttaagcaatg cgatgtcaga aaatatgcag taaaaaaaga ggttgtgaca gaagttgtga
    15961 aaaagctgtc ctgcatcaag taataagaac agccaaacaa aaatagttcc tcatgttttt
    16021 cgtttgtttg ggcttattac cgcagatgca agcttttgaa caccgtttaa gaaagaggtt
    16081 gtgggcctgc cgtttaactc taagtcacaa ggaacaattt caaaaaacag cttctcatgt
    16141 ttttgagtta gttggtctta ttacttgatg caagcttttg accactatta tgccgaacaa
    16201 ggagatgtga cacaaatttt gtcacatctc ctatttttgt ttcttgatca aaaaattctt
    16261 atacttgata ctcacactca ttttgtatgg atttagaaaa gaatatgtgt cttgacagtt
    16321 attagtaagt gagttatact aagtcgacat ttatgtgtca agacatatta ggagagagaa
    16381 aaaatgagaa aaaattcagt tcgaaaacta acgatttctg ctttattgat tgggatgggg
    16441 gtcattatcc caatggtcat gcctaaaatc atgattccac cagcttcctt tactttggcg
    16501 tctcatgtgc cattatttat tgcgatgttt tttacaccag gagttgcagt ggctgttgcg
    16561 ctaggtacga catttggttt ctttttaaca acgccggtta tcatcgcatt gcgagcattg
    16621 tcacatcttg tgtttgcgtt gattggggct tggtacttgc aaaagcatcc gagtatcgtt
    16681 ttaaaaaatg gacagtttac ttttgctaat tggcgtttcc aagggttcaa ttttgtgatc
    16741 ggtgtgattc attctttagc agaaatgcta gtagtcagca tcttttactt tattggtaat
    16801 atgcctgaaa catattattc tcaaggatat ttttatacag tattcatctt gatggggatt
    16861 ggtgggctta tccacagttt agttgattat aatattgctt attttatagc aggaacactc
    16921 agcaaatatt ttgatatccc cgtatttacg gcagcaaaaa aagaaaatcg gaaagagtcg
    16981 attcaagggt acgcgaagaa aacgacatat tagacatgat ctctcaaaaa aaaaactctc
    17041 atctagtaat ggataataaa tcctctaaga tgagagtttt ttgtcctaaa aagagctgca
    17101 ccaacatctt ctggcacagc tctaacaggg ggcaatttat ggaagtttga tgacttgttc
    17161 tgtttcttta tcaaagaagt ggcctttgct gatgttgaag gcaaggtcga ctatttcgcc
    17221 tggtttatgg aaatctcttg catctacttt tgagatgaac tcagtagaac cagtttttgt
    17281 gtaaagcatc gtttcagctc caagtaattc ggaaacgaca acttctgatt tcactacggc
    17341 ttcaggtgaa gcttcaaggg ctacttgttc actgtggatg tcttctggac gaataccgaa
    17401 gataacttct tttccttcat aacctttttc aactaatact ttgttttttc cttcaggaat
    17461 acgaagctta agcccatgtc catctgtgat aacaccattt ttcaatgtca cattaaagaa
    17521 gttcatggca ggagatccaa taaagccggc aacgaacata ttgacgggtg tatcataaac
    17581 ttctttcggt gtaccgattt gttggataaa tccgtctttc atgataacga ttcggtcagc
    17641 catagtcatt gcttctgtct gatcgtgagt aacatagatg gtcgttgttt caagacgctg
    17701 gtgaagtttg gcaatctcag cacgcatggc tacacgtaat ttagcatcta aatttgataa
    17761 tggctcatcc attaagaaga cttttgcatc ccgaacgatt gcccgtccca atgccacacg
    17821 ttggcgttgt ccaccagata aagcagctgg ttttcgtttc aagtattctg tcagacctaa
    17881 gatctctgct gcattgtcca cacgtttttt gatgtctgct ttatcatatt tacgtaattt
    17941 caagccaaaa gccatattat caaaaacagt catatgagga tacaatgcgt agttttggaa
    18001 aaccatggca atatcacggt ctttaggtgc cacgtcattc atgacttttc cgccgatatt
    18061 tagttctcct tcagagatat cttccagtcc cgcgatcatt cgcaaagtag ttgactttcc
    18121 acaaccggaa ggaccaacaa aaacgataaa ttcacggtct ttgattgata gattaaaatc
    18181 agttacagaa taattttctg cattgtcata ttttttatat acgtttttca gagccatttc
    18241 taccataata cgttccacac tttctttttt agttttatta actatttaaa gtataaatga
    18301 aagcgttaac taacttcaat gtcatgttga acaagaattt tttctttttt ttgtcaatgt
    18361 gctagtttta agctttaata aatatccata agaaataaaa actgatacac tggagtaaat
    18421 aagtatgtta acaatatcaa aaggagtgtc tcttatgaaa atcgctttga tcgctcatga
    18481 ccgaaaaaaa cctatgatgg tgaaattagc aacagcgtat aaaacaatat tggaaaaaca
    18541 tgaacttttt gctaccggga cgactgggca gaggatcacg gaagcaaccg gcttgccgat
    18601 ccatcggttt aaatccggac cgttaggcgg ggatcagcaa attggagcca tgatttcgga
    18661 ggatcagtta gatttggtga tttttcttcg agacccgctg gctgcacagc cccatgaacc
    18721 agatgtaaca gcacttatcc gtttatctga tgtctatgag attccgttag ctacgaatat
    18781 cggaacagct gaggtgctac taagaggact ggaagccggc ttcctagatt ttcgaagaat
    18841 cgtccatgaa atagacaagc atccactttc attttgatgg ataagcgtat ggaaaaataa
    18901 cgtttaatgt tcgcctatat gtagctataa aagctaaaca aactgataat atgcgatata
    18961 atacgaacgt attggataat ataaacaata atattagttt ttcgtatttg cagtaaatgt
    19021 gaaaatatgc tatagtttct gtgggaaaag atcccaaaaa taatacagag tagaaaataa
    19081 agcgttaagt gctaagagga tgggatgttg cctcttggac gaaggacgta atgttcgcgg
    19141 ttttattttc gcattcgctg catgatacga tgtagcagct cttaaaggag atgctaaaaa
    19201 tgaagaaaca ccggttagac gcacgtatga ttgctattat gggcttattg attgcattga
    19261 tggtcacttt gtcccgactg gtagcaatcg agaccccatt tattaaaatc agtgtcacat
    19321 ttattccaca agtgatcatg ggcattcttt ttggtccttt ttggtcaggg ataggcgcag
    19381 ttttagctga tttagtagga atggccctct tctcgaaatc tgcattcttt attggattta
    19441 cattgaatgc atttattgaa ggagcaatct acggattctt tttctatcga aaagagatca
    19501 cttggaaaaa tgccattctg gctacgctta gtgtcacgct gatcatcaac ttatttttga
    19561 caccattatg gcttgcgttg atgtaccatg tgccattgtt cagctgggtc gtttgggcgc
    19621 cccgcctgtt aaaaacagta atatggttac cgattcaatc aattgccatt tattacgtag
    19681 gacgtagtat tccttacaaa aaaattttaa gaagtttagc gatccacgct aaataaaaaa
    19741 caagcagtct tcccatccca gaagactgct tgtttttatt gtgctgtgaa ttgtgcttga
    19801 tagagccgtc ggtaataacc atctttttga agcagctcat catgggtgcc catttcgaca
    19861 atagctccct gatccatcac taatatctgg tccgcatttt tgattgtgga caggcgatgg
    19921 gcaatcacaa aactggtttt tccttccatc attgctaaga aggcttgttg gatttttttc
    19981 tctgtcaatg tatcgactga gctggtagct tcatctaaga tcaacatagg gggattactg
    20041 atcatcgttc gagcaatcgt caacagttgg cgttgtccat ctgatatctt taacccttga
    20101 ccgccaatct tagtatctaa tttttccggc aaacgttcta caaattcata catataagag
    20161 gcttttaggg catcattgat ttctgcgtca cttgcttccg gattcccaaa acgaagattt
    20221 tctcgcaatg tactgtcaaa tagccaagta tcttgtaaca ccattccaaa acttttacgt
    20281 aatgtatcac gttggataga gcggatgtca tggtcatcga ctgtaattgt tccttttgtt
    20341 gtgtcataaa aacgcatcag caaattaaca agtgtggatt ttccggcccc tgtttttcct
    20401 actatagcaa tggtttgacc tggataagca gtaaaattga aatcttcgat caaacgctgc
    20461 ttggggtcat aagaaaaaga aacatgatca aaagagactt ttcctttgat atttttgggt
    20521 tccaaggcat aggcatctgg caaatcaggc ttttcttctg gctgatcgat caaatcaaat
    20581 gctcgttcta gaccagaaaa agcggtttgc agttgtgtcg tgattccaga aagttcgata
    20641 aatggtttag aaaactgact tgagtagatc gtaaagctgg aaatcacacc aacagtgatt
    20701 gacgaaccac tcttcaatgc caataggccg ccaactaaac caatcgataa ataagcaagg
    20761 tgatctacaa aacgagaaga cgggtttgtc aaggaagaag aaaattgtgc ccgttgtcct
    20821 ttttcatata gatcagcatt tagcagttca aattgctgtt gactagcttc ttcttgctga
    20881 aaagctttca cgattttctg gttaccgatt cgttcggtca caaagcccga gatatcccca
    20941 ataatccttt gctgtctatc gaaatttgtt tgtgaagcac gagcaacgat ccaactcaca
    21001 aggaaaataa ttggtgtaga acaaagaacg accaatgtca gaagtggact tagacgcatc
    21061 ataaaaataa acgaaagaat gatgattgcc actccagaaa ataattgatt gaaggccgca
    21121 gaaattgcaa tggaaatatt gtccatatca tttgtaaatc gactgacgat atttccgtgg
    21181 gagttttgat cataataacg aagtggcaat cgattcagat gagcaaatgc atcttttctt
    21241 aattttgcaa cggataagta agctaaccga ttgcttaaca cttggatgag ccattgactg
    21301 actacgttga ttaaaacgat accaccgaat agaataagta aatggaacag tcgattgaag
    21361 cggacctgtc cttctccaat catttgatcg atactttgac caatttgtaa cgtcatcaca
    21421 actgttgcaa tcccattgat gattcctagt atcacagcta gccaaatgtc ttttgcatag
    21481 gcagtaaggt aaggaatgaa tcggcggagc gtctgcaagg ataatttttt tactttcata
    21541 actattcttc ctcctgtgat gcgacgattt cctgatattc tgctgattga gcaagtaatt
    21601 cttcatgggt accctgagca gcaatcttac cttgatccat cactaagata tgatgtgctt
    21661 cttgaataga gcggacacgt tgggagataa taatcactgt actgccaaga taagtctgta
    21721 gcgccttacg caaatccaag tctgtttgat aatccaacgc acttaaagag tcatctaaaa
    21781 tcagtaatgc tggcttggca atcaaagctc ttgcaatcgt cagtcgctgt ttttgtccgc
    21841 cagagaaatt tttcccattt tccatgactt ttgtatctaa accatttgga agctgacgga
    21901 caaattcttc tgcctgagcg attttcagag cttcccaaca ttctttttct gtggcgtctt
    21961 tcttacccca ttgaaggttt tcacgaatag tcccggtaaa tagtacagaa gtttggggaa
    22021 caagagcaat ttgctgacgg agatgatcca gctgccaaga acgaacatcc attccgtcca
    22081 ttataactgt accggaactg acatcataga aacgcggaat caattgtgtc aaagtcgttt
    22141 tcccgcttcc ggttggacca atgattccaa ggactgtatt tcgcttcagc tgaaatgtaa
    22201 tattttggag agaaagacca gaatcaggag tataacggaa gtcaacatgg tcaaaagcga
    22261 taattgcttc agaagaaaaa tcgggagtag tactgacctc agcttcaaga atcgaatttt
    22321 ctgtgtctag cacctctttc acccgatttc ctgaagcttc ggcacgtgtg aagatcacga
    22381 cgaggtttga aacaacgatc agtgccaaaa gcatttgatt catataattg atcaaagcaa
    22441 gaacttgtcc ttgttgcaag ctaccaatat ttactttgat cccgccaact gtcagcaaac
    22501 agatgatccc gacattcatg atcaaggttg tagcaggtga taatagggca gagatattgg
    22561 aaacacgtaa ataattgttc gctagatcat ttgtgctttt atcgaacttc tctgtttctt
    22621 tttttgtctg cgcaaatgcg cggatgacac gaacaccgct aagattctga ctaacgtgtt
    22681 cattgagctg atctagtttt tcttgcactt tttggtagag aggaacggtt tttttgatga
    22741 taaaaaataa aatgattgag aaaataggca ataatgctaa gaaaaacaga cctacttccc
    22801 agtcgatcac aaaagccatg accactgacc cgatacttaa aaacggtgct ctgatcaata
    22861 aacgaatgac cattgctaaa gctagctgaa gctgattgat gtcgtttgtc attcgggtga
    22921 tcaaggtatc tgttccaaaa tgattcaact cgttgtgaga gaaagtattg atttttttga
    22981 ttagctgatt acgtaactct gtaccaaatc cttgtgaagc tactgaagca tagtattggc
    23041 aaatgatagc acaaatcaga ccaatcacag acatagcgac catccataag cccatttcaa
    23101 tgatttttcc tcggtcattc atttttagtc cattatcgat caaggaagcc atcattaaag
    23161 gcaggacaag ttcgaagaca gcttcaagaa atttgaagaa cgggcccaaa atgatttgct
    23221 tacgatagtc ttttgcataa cgtaataact gaatcatatg attcctccta ttatttattt
    23281 gtccacaatt gaaaaaacaa gtactaccac attgtagctt aggaaattta gaatgaaaag
    23341 agaacaagcc ttttcataaa tgaaagtttt attatgaaga agctttagaa attgaataga
    23401 ctattttttt taaaaaaaag aaaatgagaa aacatgccag tctcttcatt cataggaacg
    23461 tctattttac ttaatttttc tataaatgaa ttctgcataa a
//
LOCUS       DAZKLM010000041        23414 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3764
            SAMEA115706913-rid22582563.denovo.041, whole genome shotgun
            sequence.
ACCESSION   DAZKLM010000041 DAZKLM010000000
VERSION     DAZKLM010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706913
            Sequence Read Archive: ERR13196647
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23414)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23414)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287823.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287823.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,004
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,849
            CDSs (with protein)               :: 2,849
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 41 of 96
            Pseudo Genes (incomplete)         :: 52 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 15 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23414
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706913-rid22582563.denovo.041"
                     /isolate="VRE3764"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(33..1076)
                     /locus_tag="ACG842_001451"
     CDS             complement(33..1076)
                     /locus_tag="ACG842_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HFQ0248001.1"
                     /translation="MKKILVSLLFLVSLVTLVGCSQSQGEKQSEKEKSKIALVLGFGG
                     VDDRSFNQAGWEGLEAWGKEKKLTAKKDFTYIETNNPEDNSTNIDQAIRNGFTTIISL
                     SYLQAEELENAAKDNPKLKFALVDGEITGKKNISSATFKDQESAYLAGLAAAYTTKSD
                     QVGFIGGMEGPVIQRFQSGFEAGVKAGATALNKEITVNVQYAASYDSLDKGKAIAASM
                     YKEGVDILYQAAGSTGAGVFQEAKARNENDYSRKVWVIGVDRDQADEGDYKMENGETG
                     NFTLTSTKKNVGAEVKEFAETSHNDKFPGGKHLTFGLKEEGVDLTKGQLSEEAYQAVS
                     DAKEKIISGDFRL"
     gene            complement(1237..1920)
                     /locus_tag="ACG842_001452"
     CDS             complement(1237..1920)
                     /locus_tag="ACG842_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HFQ0248002.1"
                     /translation="MKKIHDTKQLTAYVEKHNLQDYMDSDILAMASLLFFEKEEHLIQ
                     TGKTSDYLYFLVDGTVMVYSYSSDTQNICIDYAKPATPLGEASSLWELSPKSSVKAVT
                     KCICISIPLNQYRTQLQKDVRFLQNICQLLSYRLNSGINLANSLTEPVETRLAKFILT
                     HHENERFSFRLTTCASILNVSYRHLLRTITNFREENIIEKKKNYYLIHDIEALEDLAE
                     NISSPVKAE"
     gene            2115..3044
                     /locus_tag="ACG842_001453"
     CDS             2115..3044
                     /locus_tag="ACG842_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010743455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate/phosphite/phosphonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HFQ0248003.1"
                     /translation="MQFKQLAKGFIGVMLLTTLAACGSGNTSSSSEKESKEKLVVQFV
                     PTNNDGSMEAKAKPFAKYLSKKLDREVEVTLATDYSTIVEAMSSGQVDVGIMPPAAYV
                     QAKDAGAADAILTSQLGDYDQETGLPLKDKLTNTFKGEVLVRADSGLNKLTDLKGKNI
                     ATLSPNSASGYIYPVAELKDAGIDPTADTKMTTVNDIPSEITAVLNKQMDAAFVFEGA
                     RNVFASNFPDNDLFKDLKVLYLTKGDIPNDAIAVQPDMSKDLKAKIKETFLNMKDDKD
                     GKEAMALWGHQGYEEAADSAYDTIRDYTKKAAE"
     gene            3105..3896
                     /gene="phnC"
                     /locus_tag="ACG842_001454"
     CDS             3105..3896
                     /gene="phnC"
                     /locus_tag="ACG842_001454"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015416 - ABC-type phosphonate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter ATP-binding protein"
                     /protein_id="HFQ0248004.1"
                     /translation="MIIFDNVSKVYPNGVVGLKDIDLTIEDGEFVSIIGLSGAGKSTL
                     LRSINRLAETTKGDIRINGTSITKANKKELRQIRRQIGLISQSFNLVKRSTVQKNVLS
                     GRLGYYSTWKSILGLFTKEDYQQTKEALEKVGLSDKLQTRSDELSGGQQQRVSIARAL
                     VQQAPIILADEPVASLDPITTQKVMMDLKNINQTMDKTVIVNLHSVPLAREFSTRVLA
                     LKAAQVVFDGRPDDLTDERLEQIYGNAIFEEKAGEIDELERNRSL"
     gene            3868..4647
                     /gene="phnE"
                     /locus_tag="ACG842_001455"
     CDS             3868..4647
                     /gene="phnE"
                     /locus_tag="ACG842_001455"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010743453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015416 - ABC-type phosphonate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HFQ0248005.1"
                     /translation="MSLKETVHFKWYKHLLILLFLIVCFQGSAWITSADFSQVFQNSN
                     QMTVFLSRFIHPDFSYLPKLVEPMVKTLQMSLLGTVIGLILAIPVSFLATTVVTENRL
                     LSTIFRFLLGLVRTIPTLLLAALFVAVFGIGEATGVLTIAVFTFGMVSQLMFQAIETI
                     DFGPIEAAAAVGANKMQIAIWSIAPQVLSQFASYAFYAFEVNVRASTVLGYVGAGGIG
                     VILNSSLALLNYERVSIIILTILVTVAIVDKLSERVRRSLL"
     gene            4644..5426
                     /gene="phnE"
                     /locus_tag="ACG842_001456"
     CDS             4644..5426
                     /gene="phnE"
                     /locus_tag="ACG842_001456"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005233297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015416 - ABC-type phosphonate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HFQ0248006.1"
                     /translation="MMLREMNRRTLLSLVALVILVILSAATLDYSGLSTFSPSMGMDV
                     LRGLGQPDWQFLYDGSGEDLISLLLLTIGIACLGTMIATVLAIPITLISAVNLWQAHP
                     WVTKIGKLICNVLRAFPELVFAIIFVKVVGPGPFAGVMAIGVHQIGMLGKLFTEEMEA
                     MDERLVEEMHAVGANFWQTIFFVRIPYLMPIYCSLALNHFEIAVRSAATLGLVGAGGI
                     GAPLIFAIQTRSWSKVSIILIGVVVTVFILDQITGIIRKRLR"
     gene            5429..6232
                     /locus_tag="ACG842_001457"
     CDS             5429..6232
                     /locus_tag="ACG842_001457"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFQ0248007.1"
                     /translation="MNILHISDIHFRREYEACEEGYKGMLATMQNPLIPLEQCIHHLL
                     QQTPLDLVIISGDLTEDGEIDDYRYLKTWLENALGETTILVTLGNHDIKEHFWVGWCD
                     EAASSAPYNQIVKYPEFTVISFDNSSYGYADGIVDDQQFQWLKKALEQEKDQPIFLVT
                     HHHLLSHQSSIPKWPGTERFLKLIAPYDIRCILNGHTHHTFTDEINGIPYFTVSSMSF
                     VGEDEGDGFVRFEERHGYNLYHLDQGRIVRQTTENFIPGNILKTLQMKD"
     gene            6316..7863
                     /locus_tag="ACG842_001458"
     CDS             6316..7863
                     /locus_tag="ACG842_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HFQ0248008.1"
                     /translation="MNITFLETSDMHGYVSPTDYSGRTDLALGTAKVSSKLKELRAKA
                     DGPVITIENGDFIQGSPLSYYLAKEYGSAKELTDLINQMSYDVQVLGNHEFNYGVDYL
                     EEAIKNYQAPILGANMVNKNDQPYFGNAYKIIEKVGVKIAILGLTTQYIPHWEKPETL
                     AGITFKSIVETAKEYVPLLRKQADLVIVSYHGGFEKDLLTGEDTEALTGENEGYELLQ
                     QVEGIDALFTGHQHRKIAETVHGVPVVQPGYRGEFIGEIRLTVEKKEGQVVVTDHQVA
                     LHSVEKSEPDPEILAAIVPVEEKLEKWLDQPLGKVNGNMRINDPMQARLEEHPYVEFI
                     NRVQLFASGSEISGTALFNNEGKGFGETITMRDVITNYIYPNTLAVIQINGADLRAAL
                     EQTANYLAVENNQIVFNPAFIQPKPQYYNYDMYEGIQYTIDMKQPVGQRITELRFKGH
                     PVLPQQMLEVVTNQYRAIGGGNYAMFGAEKIVREIQIDMTELIADYLNIHPRIEAKVN
                     HNFQVIL"
     gene            complement(8160..8357)
                     /locus_tag="ACG842_001459"
     CDS             complement(8160..8357)
                     /locus_tag="ACG842_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0248009.1"
                     /translation="MVECLKNKKMEANFVKAVISGSIVFQEEYRDMEKDFSKKGIELI
                     DYPRSCPILDKEYPEILTTFF"
     gene            complement(8433..8633)
                     /locus_tag="ACG842_001460"
     CDS             complement(8433..8633)
                     /locus_tag="ACG842_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HFQ0248010.1"
                     /translation="MIEEEQKNIWDRYYKADPSSKNTKIGESGLGLSIVKELVRLHHG
                     SIEVTSRKNEGTTFLIQFPKEK"
     gene            complement(8753..9940)
                     /locus_tag="ACG842_001461"
                     /pseudo
     CDS             complement(8753..9940)
                     /locus_tag="ACG842_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(9985..11111)
                     /locus_tag="ACG842_001462"
     CDS             complement(join(9985..10883,10883..11111))
                     /locus_tag="ACG842_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611172.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFQ0248011.1"
                     /translation="MTRYEENFKQMIVELNQTGRSVRGLAKEYGLSEATIYKWKNLYL
                     PDQSTGLTGKEVAELRKENARLNEELEILKKAGSHILSENLNSLVQFIEKWCKDYTVS
                     LLCRLLEIPRSVYYFYKNKPLTATEIRNNKLKKKISTIFFTNKQRYGATKIHQVLLKE
                     GISVSLKHVQKLMKQLNLRSIVVKKYRPQRSNKPIISKENLLNQDFSTETICEKWAAD
                     ITYIPTKKNGWCYLSSIMDLHTKKIISYTFSKRMTVDCVIQTLNKAKIHYHIPEGMIL
                     HTDLGSQYTAREVEQWLKTNKIRHSYSRKGTPYDNAGIESFHASLKKEEVYTTSYSDF
                     EEANRALFSYIEGFYNRNRIHSSIHYLTPQEFEELAKEKMA"
     gene            complement(11515..12297)
                     /locus_tag="ACG842_001463"
     CDS             complement(11515..12297)
                     /locus_tag="ACG842_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0248012.1"
                     /translation="MENSSNSAEVNVSTSTSEVVKCSQETTSLPEELLGDWQINNKDA
                     GIEISDSKAVFRIGRSQVESKDYYVDVNNKMLLHVYFSFEYDSVKYIEVSIERTGYSE
                     FISKSVGILTMHTKDGQYISLGVKRVSGENNETEAVTEQTMDERIDSSKSNETEVDTG
                     KTIDERIDSFIETYTAIPPTWKLVNEDKGITINTDTEISTDAQSLSDLLANLTGISNA
                     VTEQVGKDIPLRLLENGKKDYSVVFKNGQVTDYNEVFKKVIE"
     gene            complement(12362..13198)
                     /locus_tag="ACG842_001464"
     CDS             complement(12362..13198)
                     /locus_tag="ACG842_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFQ0248013.1"
                     /translation="MRVIKTLNNSLILCLDKQGKEVILMGKGIGFQLKKGDLVPADKI
                     QKVFKLDSLSDALKKDYIKLFEAAPDDLIICVKRIIDYANAKFDFKLNKSLFFTLVDH
                     LKHAIERSQKGIVFQNRLLIEIQKYYPKEFEIGCYAIQLINKKMNVQLPEEEAGNIAF
                     HIVNAQSEQANMEETMLSVRALKDILAMLGYLFPEKKVDKDSMLYLRFVTHLQFFIAR
                     MIKKEELPEKDDFLLASVKQRFPKEYRAALKIKEYVQEEMGASANQDELLYLTLHISR
                     VY"
     gene            complement(13216..14625)
                     /locus_tag="ACG842_001465"
     CDS             complement(13216..14625)
                     /locus_tag="ACG842_001465"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010757186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFQ0248014.1"
                     /translation="MQYKQLSAFPEKFLWGASISAYQAEGAYQEDGKQPSIIDKYQHT
                     EGVANFEVASDFYHHYKEDIQLFKELGLKAFRFSIAWTRIMDIENNKTNQKGISFYHQ
                     VIDECLNQGIEPIVTMYHFDLPYFLEEQGGWLNRATIDYFVDYVQVLLTEYGDKVNYW
                     LTINEQNTMILHPGAIGLPPSGELPSKKELFQINHHVLLAQARVIHLYHQLNLKGKIG
                     PAINLTAMYQATSSPEDAIAAHNWETLRGWSFLDVAVRGKYNYLFENYLNDRGLYPKI
                     EAEDQAILSSGKPDFIAINYYSTATIAASKNDGSDVSARAGDQQIMLGEEGVYRAAEN
                     PYVDKTPYGWVVDPTGLRLTLRKLYDRYDLPILITENGYGAPDIVEEDRRINDQDRID
                     YLAKHILAIQAALTDGVDVFGYLPWSAIDVVSTHQGFNKRYGFIYVDRTDENLKELKR
                     IKKESFFWYQKVIARNGLA"
     gene            complement(14639..16462)
                     /locus_tag="ACG842_001466"
     CDS             complement(14639..16462)
                     /locus_tag="ACG842_001466"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
                     /protein_id="HFQ0248015.1"
                     /translation="MDYREVGRNVLQQVGGKENVVSLTHCATRLRFELKDKRKADTKA
                     LEKTLGVISVVDSGGQYQVVIGNEVQTAFKEIQKEVGTSIKKGPSSDNQESIISRLIS
                     VISTTFTPMIPAITGAGMVKAILAVLTLTGVLSDSSQTYIILNTIADAAFYFMPVLLA
                     YGAAIKFDVNPILAITVAGILLHPNISQLFSTGDAVSFLGIPVLAADYAGSVLPIIFT
                     VWIMSYVEPFADRISPSFIKFFTKPMLVFLIMGPLALVVIGPFGTLLNDLIAMGAEWI
                     NGKAAWLIPFLMGGLQPFLVVTGTAWAMTPIATMQLSSTGSEMINGPGMLASNIAQSG
                     ATFAVAAKTKNKELKQLAVSTGITALMGITEPSLYGVTLKLKKPLVASMIGGAIAGLY
                     AGLSGLVRYAFVSPGLAALPAFIGENPMNIVHAIITCVIAFVATFIIAYLIGFDDTSD
                     ESEQTAIAEAVIVENPVKGTVCPISEVNDAVFSKGLLGKGVAIHPEENSIYSPVEGEV
                     ITILESKHAIGLRSKEGIELLIHVGIDTVNLKGEPFEVFVKAGDQVSAGQLLINADFA
                     AIRNAGYDDTVIVVVTNSENYAGISEASGSLKEHEPLMQIS"
     gene            complement(17305..17631)
                     /locus_tag="ACG842_001467"
     CDS             complement(17305..17631)
                     /locus_tag="ACG842_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0248016.1"
                     /translation="MNKVNMNKKFIIIVIVVLAIIGIIFSKMTGTSGLEKSSKPLVTQ
                     ILQEQYNLNRSCDDVTITHENGDNTYRAKAILDNGSAININIEYYPKKDRIYVEIPYA
                     EVLMLN"
     gene            complement(17677..18039)
                     /locus_tag="ACG842_001468"
     CDS             complement(17677..18039)
                     /locus_tag="ACG842_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010717431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HFQ0248017.1"
                     /translation="MYCRKCGKEITDESKYCQFCGALQETDETTGADAVKKSTEQTIN
                     EMKEQIVHGQETGKLYLIIGWISMVVALAFIPVVFGAVAVIMGYLYRDKDEQMGTILM
                     IAGVAGAIFGVLLGMSMY"
     gene            complement(18076..18290)
                     /locus_tag="ACG842_001469"
                     /pseudo
     CDS             complement(18076..18290)
                     /locus_tag="ACG842_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324259.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            18418..18753
                     /locus_tag="ACG842_001470"
     CDS             18418..18753
                     /locus_tag="ACG842_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFQ0248018.1"
                     /translation="MKQKQYAKVIREKRKALGWTQKELAEKIFSTQQAVARWENSVTE
                     PNLDSLTALSRALGTPVSHFLDNVVVDYEEEFLALYRSLSTEDAVRTIDYMKLLKRQE
                     NERNQLLKD"
     gene            complement(18829..20826)
                     /locus_tag="ACG842_001471"
     CDS             complement(18829..20826)
                     /locus_tag="ACG842_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0248019.1"
                     /translation="METSMINIPIYVDKEKLLEKPVTKKEKTDVRDRCLDNYREVTRD
                     EFVEILNSSQSFIPSKTKNKGNDKESFVETRVIILDVDNTVKDEKGKVVDLSKDDSRY
                     LSIEKVLSINLVHNSAFAIQKSIRYSENLEKYKIVFLLKEAITDYGKMVAVYEYLKKN
                     IPGCDDNVNASNRMFFGGYKSDAVIIINEDNMLDITELPIDFTMTCSKNCQSDSGREY
                     ELVNETDFVKLIKNDDKEEMKQWFKDCLFDSSDMSFNEIYERLLTIDMNKLLKSSKNL
                     RCLFHNDHNPSASIFTSKTGHSIFYCHSSNCDISENFIGVVMLILKKDSRVETFNWLL
                     ENLDLYPTHFRKLKEESKVLFDTLESMKDKLFRTIRNYLEEMRRVYDVLLSDVNFFDD
                     SKESVTCILSGEQLAKKMSFYYGKTYDIDKCNKLLSLMTFIGLIKKLDDEDVPLKMLE
                     YLNRLKEEKALGNFNHKRSNVYRLDVFNATSVIQKLNEDILPLLEKFHFTYQYFSYEW
                     VKICFNEKEAKRVFPQNSRSELSKEKQLILDEAHEVLQGLSWQGTYLMTEKELTNTVC
                     ERLRNVGNNSVENALKENRGYFVKQGFTLERASKDIKRLYNVKASSSFLVYLAKGAGI
                     DEVIKELILSGVELVPLYQFTSVTRDDGRRVRKRKDPIVLN"
     gene            complement(20924..21199)
                     /locus_tag="ACG842_001472"
     CDS             complement(20924..21199)
                     /locus_tag="ACG842_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0248020.1"
                     /translation="MSLQIIDSIVSIQVEQQVKEIITVLKPMLEDIISGVTLEYMDYQ
                     QASEYLGVSVGTIRKYVSQYGLPVIKIDTVIRFKKSDIDSFMERYKG"
     gene            21431..22165
                     /locus_tag="ACG842_001473"
     CDS             21431..22165
                     /locus_tag="ACG842_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFQ0248021.1"
                     /translation="MIKRPPINYLERKKILGTKIKAIRKSKKLTQPAFGLMINNGQLI
                     DKKTIYEWEKGTYLPIPERLSRIADLGNMSIEELVCGNVEEYILGIILYRDSIVLDGI
                     TFPDKNLFQHLRQQFPPVHSNLDTWLDRYSKLEPEMQEFIANKTCNKVKNEKISLFNI
                     LKIEELFINAIVEEFDNNILFLTSSIEELLERMVDEWLPIQLKDMSYPEEAVREITDN
                     INKLEQTISSIGKKYTKKKMKGGDTI"
     gene            22204..23346
                     /locus_tag="ACG842_001474"
     CDS             22204..23346
                     /locus_tag="ACG842_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFQ0248022.1"
                     /translation="MAKISKYKKKNGQVAWMYKGHIATDPRTGEKINTTRRGFSSKQE
                     AQKDYDEYRHQILYGVKKKAPDMTFEDLYNEWITHQRTSVKASTIAISVRYANNQILP
                     AFGKLKLSNISVPYCQKVVDEWHSKYESYDYMRKQTAQILRYGVAMQYIDNNPMEKTL
                     LPRKKEYEKNRKFYSKEELNNLLDAFKDFGNMKQYAFFRLLAYTGMRKSEVLALQWKD
                     IDTFNKELHVNKTLAVDEFGKVIIQSPKTRASRRVISLDTETLSILNNWKLQQKEEYL
                     KLGYNTSSKEQHVFTTVKNTLYIPNTVNDWLRYILKKYNLPRITPHGFRHTHASLLLE
                     AGESVKVVQQRLGHENSKVTLDIYAHITNNAPKKTGQDFADMMAHQ"
BASE COUNT         7224 a         4623 c         4094 g         7473 t
ORIGIN      
        1 actgtagaca tcatccataa aatggacttt gtctacagtc tgaaatcacc actgatgatt
       61 ttctcttttg cgtctgaaac tgcctgatag gcttcctctg ataattgtcc ttttgtcaga
      121 tctacccctt cttctttcaa accaaaggtc aaatgcttgc cgccaggaaa tttatcgtta
      181 tgagaagttt ctgcaaattc tttgacttct gcaccgacat ttttctttgt agaagtcaac
      241 gtgaaattcc ctgtctcgcc gttctccatt ttataatccc cttcatccgc ttgatcgcga
      301 tctacaccaa tcacccatac ttttctgcta tagtcgtttt cattgcgggc ttttgcttct
      361 tggaacaccc cagctccagt acttcctgcc gcctgataaa gaatatcgac tccctctttg
      421 tacatagaag cagcaatagc ttttccttta tctagcgaat cataggatgc agcatattgt
      481 acgttaacag taatttcctt gttcagtgct gtggcccctg ccttaacgcc agcttcaaat
      541 ccgctttgga aacgttggat cacgggccct tccattccac caataaagcc tacttgatca
      601 ctttttgtcg tatatgcggc agctagtcct gctaaatacg cactttcttg atctttaaag
      661 gttgctgatg agatattctt tttcccagta atctcaccat ctaccaaggc aaatttcaac
      721 tttggattat ctttagcagc attttccagt tcctctgctt gcaagtagct caagctaata
      781 atggtggtaa agccgtttct gattgcctga tcgatattgg tactgttatc ctctggattg
      841 ttcgtttcta tatacgtaaa atcttttttt gctgtcagct ttttctcttt tccccaagcc
      901 tctaatcctt cccagccagc ttgattgaag gagcggtcat caaccccgcc aaatccaaga
      961 accaaagcga ttttactttt ctctttttca ctttgcttct ccccctgaga ctgactacag
     1021 ccgactaacg ttactaatga caccaaaaac aacaatgaaa caagtatttt cttcaaaatt
     1081 tttctcccct ttttatgtgt cttgacacat tgaactttta aaagtatagc ataccaatat
     1141 atagaacact actcttcaac gaagattttc cgtttcccaa tttctccact atcaaaaaac
     1201 cagcaatcac ttctgactgc tgttttttta tagctgttat tctgctttta ctggactact
     1261 tatattctca gctaagtcct ctaaagcttc gatatcatga atcaagtaat aattcttttt
     1321 cttttcgata atattctcct cacgaaaatt cgtgattgtt cgtaaaagat gtcgataact
     1381 gacatttaag attgaggcgc aggtggttaa ccgaaatgag aaacgttcgt tttcgtgatg
     1441 ggtcaaaata aactttgcta aacgagtttc aacaggttct gtcagtgagt tcgccaaatt
     1501 tatcccggaa tttaaacgat aacttagtaa ctggcaaata ttctgcaaaa agcgaacatc
     1561 tttttgaagc tgtgttcgat attgatttag cggaatgctg atacagatgc acttagtcac
     1621 tgctttaacg ctgcttttcg gcgataattc ccacaatgaa gaggcttcgc cgagtggtgt
     1681 agcgggcttt gcgtaatcga tgcaaatatt ttgtgtatca gaagaatacg agtacaccat
     1741 taccgtccca tccactaaaa aatacaaata atctgaggtt tttcccgttt gaatgaggtg
     1801 ctcttccttt tcaaaaaaaa gtaaagaagc cattgccaat atatctgaat ccatataatc
     1861 ctgcaggttg tgcttctcaa cgtaagcagt taattgcttc gtatcatgga tttttttcat
     1921 gaaatcgtca cctctaagaa gtttttcttt attatagctt agattgtttt tcggctcaat
     1981 acgaataatt atttataatt gtttgtaaat tgtatgtaaa agttgtgtaa aataggacgt
     2041 gcgtcacttc attgattttg attccctgct atgatttaga cgtattagaa attatatcca
     2101 acaggaggag aaaaatgcag ttcaaacagt tagcaaaagg gttcatcgga gtcatgttat
     2161 tgaccacttt ggcggcctgc ggttctggga atacaagtag cagctcagaa aaagagagca
     2221 aagaaaaatt agttgttcaa ttcgttccaa caaacaatga tggtagtatg gaggcaaaag
     2281 caaaaccatt tgctaagtat ttatcgaaaa agttagatcg tgaggtagaa gtgacgttag
     2341 ctactgatta ttcaacgatc gtcgaagcga tgtcttctgg tcaagtcgat gtcggaatta
     2401 tgccgcctgc agcctatgtt caagcaaagg atgctggagc tgcagatgca atcctgactt
     2461 ctcagctcgg cgattatgat caagaaactg gcttgccatt aaaggacaaa cttacgaaca
     2521 cctttaaagg agaagtattg gttcgtgccg atagcggatt gaataagtta actgatttaa
     2581 aaggaaaaaa tatcgcaacc ttaagtccta actctgctag cggctacatc tatcctgtcg
     2641 ctgaattgaa agatgctggg attgatccta ctgcagatac aaagatgacc actgtgaatg
     2701 atattccgag tgagattacc gcagtgttga acaagcaaat ggatgcggcg tttgtttttg
     2761 aaggcgcgcg aaatgttttt gcttcgaatt tcccagataa tgatttattt aaggatttaa
     2821 aagtcctgta cctgacaaaa ggcgatattc cgaatgacgc aatcgctgta cagcctgaca
     2881 tgtcaaaaga tttaaaagcg aaaattaaag agaccttctt aaatatgaag gatgataaag
     2941 atggcaaaga agcaatggct ttgtgggggc atcagggcta tgaagaagct gctgattctg
     3001 cctatgacac gatcagagac tacacgaaaa aagcagcaga ataaaacatc agtaagtgtc
     3061 agcaagcttt tttttgctta cacttaaaaa aatggagaag gataatgatt atttttgaca
     3121 atgtttcgaa ggtctatccc aatggcgttg ttggcttgaa ggatattgac ttaacaattg
     3181 aagatggaga atttgtttcc atcatagggt tgagtggtgc tggaaagagc actctgctgc
     3241 gcagtatcaa tcgcttggca gaaaccacca aaggagatat tcgcattaat ggaacctcta
     3301 ttacaaaagc gaataaaaaa gagctgcgac agattcgccg ccagattggt ctaatctcgc
     3361 aaagcttcaa tctggtaaag cgtagtacgg ttcagaaaaa tgtgctatct ggacgtttgg
     3421 gctattactc cacttggaaa agtattttag gtctttttac gaaggaagac taccagcaga
     3481 caaaggaagc actggaaaaa gttggtttgt ctgataaatt gcagacacgc agtgatgagc
     3541 tcagtggtgg gcaacaacag cgagtatcca ttgcgcgagc actcgtgcag caagccccca
     3601 ttattttagc ggatgaaccc gttgcatcat tagatccaat cacgactcaa aaggttatga
     3661 tggacctaaa aaatatcaat caaacgatgg ataaaacagt cattgtcaat ctgcattcag
     3721 tccctctggc tcgtgagttt tctacgcgtg tgctcgcgtt aaaagctgct caggttgtct
     3781 ttgatggtcg tccagacgac ttaaccgatg aacgcttaga gcaaatttat ggcaatgcaa
     3841 tctttgaaga aaaggcaggc gaaatcgatg agcttgaaag aaaccgttca ctttaaatgg
     3901 tataaacacc tgcttattct tctgttctta attgtttgtt ttcaaggaag tgcctggatc
     3961 acgtcagctg atttttctca ggtttttcaa aactccaatc aaatgactgt ctttttatca
     4021 cgcttcattc atccagactt tagctattta cctaaattag tcgagcctat ggtgaaaaca
     4081 cttcaaatgt ctttgcttgg aacggtcatt ggcttaattt tagcgatccc tgttagtttt
     4141 ttagcgacca ctgtagtgac agaaaaccgt cttctttcta ccatctttcg ttttctttta
     4201 ggtttagtac ggaccatccc taccctgctt ttagccgcgc tttttgttgc tgtgtttgga
     4261 attggtgaag cgactggcgt gttgactatc gctgtcttta cttttggtat ggtgtcacaa
     4321 ttaatgtttc aagcaattga aaccattgac tttggcccga ttgaagccgc tgcggctgtt
     4381 ggagcaaata aaatgcaaat cgctatttgg tcgatcgctc cacaagtttt gagtcagttt
     4441 gcgagctatg ctttttacgc gtttgaggtg aatgttcgtg cttctactgt ccttggatat
     4501 gtcggagctg gtggtattgg cgttattcta aattcctcac ttgcgctttt gaattatgaa
     4561 cgagtttcaa tcattattct aacgatccta gtaactgtcg cgatcgttga taaattaagt
     4621 gaacgtgtca ggaggtccct gttatgatgc ttcgtgaaat gaacagaaga acccttttat
     4681 cattagttgc tttagtgatt ctcgttattt tgtcggccgc tactctggat tactctggat
     4741 taagcacctt ctctccttcc atgggaatgg acgttttgag agggttaggt cagcctgatt
     4801 ggcagtttct atatgatggc agcggcgagg atttgattag tttgctatta ttaaccattg
     4861 gaatcgcctg tttagggaca atgatcgcta cggtattagc gattcccatt accttgatca
     4921 gtgcggttaa cctctggcaa gcacatccat gggtaacaaa aattggcaag cttatctgta
     4981 atgttttacg agcttttcct gaactagttt ttgcaattat ttttgtgaaa gtcgtcggac
     5041 ctggcccctt tgctggtgtt atggcgattg gcgtccatca gatcggaatg cttggcaaac
     5101 tttttacaga agaaatggaa gcgatggatg agcgacttgt tgaagaaatg catgcggtag
     5161 gagcaaattt ttggcaaacg attttctttg ttcgcatccc ctatttgatg ccgatttatt
     5221 gttctttagc attaaatcat tttgaaatag cggttcgtag tgcggctacc ttaggcttag
     5281 ttggcgctgg cggaatcggt gctccattga tattcgcgat tcaaactcgg tcttggagca
     5341 aagtgagtat tattttgatt ggcgtagtcg taaccgtgtt tattttagat caaataaccg
     5401 gaattataag aaagagattg agataagaat gaatatattg catatatcag atatacactt
     5461 tcgcagagag tatgaggctt gtgaagaagg ctataaggga atgctggcta cgatgcagaa
     5521 tccccttatc cctctggagc aatgcattca tcatctcctg cagcaaacac ccttggacct
     5581 tgtgattatc agcggagatt taaccgaaga tggcgaaata gacgattatc gatacttgaa
     5641 gacttggctg gaaaatgcgc taggagaaac gacgattctt gttactctag gcaatcacga
     5701 tatcaaagaa catttttggg taggctggtg cgatgaagcc gcctcttctg ccccgtacaa
     5761 ccagatagtc aaatatcctg agtttacagt gatttccttt gataattcaa gctatggata
     5821 cgcagatggt atagtggacg atcagcaatt tcaatggctg aaaaaagcct tagagcaaga
     5881 aaaagaccaa ccgattttct tggtcaccca tcatcatcta ctgtctcacc aaagcagtat
     5941 accaaaatgg cctggtaccg aacgtttctt gaagctaatc gcaccttacg atattcgctg
     6001 tatcctaaac ggtcataccc atcacacatt tactgatgaa attaatggga tcccctattt
     6061 tactgtatcg agtatgtcct ttgttggtga agatgaaggc gatggcttcg tacgctttga
     6121 agaacgtcat gggtataacc tttatcatct cgatcaagga aggattgttc gccagacaac
     6181 agaaaacttt attccaggca acattttaaa aacacttcaa atgaaggatt aaggcattat
     6241 aaatcaaaaa aagagaccga acagctgaat acgcttcggt ttttctattt tgagtcagct
     6301 aaaggagcac gcagaatgaa cattacgttt ttagagacaa gtgatatgca tggctatgtt
     6361 tcaccaacag actattccgg cagaaccgat ctggccttag gaacggctaa agtatcgtcg
     6421 aaactgaagg aattacgggc aaaagcagat ggaccggtaa ttacgatcga aaatggtgat
     6481 ttcatccaag ggtctccttt gagttattat cttgctaaag aatatggttc agccaaagag
     6541 cttacagact tgattaacca aatgagctat gatgttcaag tgttgggaaa tcatgagttt
     6601 aactatggtg tggattatct cgaggaagcg attaagaatt atcaagcgcc tatattagga
     6661 gccaatatgg tgaacaaaaa tgaccaacca tactttggaa acgcctataa gattatcgaa
     6721 aaggtgggtg taaaaattgc tatcttggga ttaacgacac aatacattcc tcactgggaa
     6781 aagccagaaa ccttagcagg aattacgttt aagagtatcg tcgaaacagc aaaagaatat
     6841 gttcctctat taaggaaaca agcagacttg gtaatcgtta gttaccacgg cggatttgaa
     6901 aaagacttgt taaccggtga ggacacagaa gcgctaactg gagagaatga aggctacgag
     6961 ctattgcagc aggttgaagg cattgacgcc cttttcaccg gtcatcagca ccggaaaatt
     7021 gctgaaacgg ttcatggagt tcccgttgtt caaccaggct atcgtggcga gttcatcggt
     7081 gaaattcgtc ttaccgtcga gaaaaaagag gggcaagtgg ttgttacaga tcaccaggtt
     7141 gctttacact cagttgaaaa atcagagcct gatccagaaa ttcttgcagc gatcgtaccg
     7201 gtagaagaaa aactagagaa atggttggat caacccttag gtaaagtgaa cggaaatatg
     7261 cgtattaatg atccgatgca ggctcgtctt gaggaacatc cctatgtaga gtttattaat
     7321 cgtgtacaat tatttgccag cggctcagag atttctggaa ctgcgttatt caataatgaa
     7381 ggaaaaggct ttggtgagac gatcacgatg cgggacgtca tcaccaacta tatttatccg
     7441 aatactctgg cagtgattca aataaatggt gcagatttga gagcggcgtt ggagcagacc
     7501 gcaaattatt tagctgtaga aaataaccaa atcgtattca atccagcctt tattcagcct
     7561 aagccccagt actacaatta tgacatgtat gaagggattc agtacactat tgatatgaaa
     7621 cagcctgttg gacaacggat taccgaactt cgttttaaag gtcaccccgt tcttccgcaa
     7681 caaatgctgg aagtggtgac aaatcaatat cgggcaatcg gcggaggaaa ttatgcgatg
     7741 tttggtgcgg agaaaattgt ccgagaaatc caaatcgaca tgaccgaatt aattgccgac
     7801 tatttgaata ttcacccacg catcgaagca aaagtgaatc ataattttca agtgattttg
     7861 tagtatataa ataaaaaatt caaaaaggtg tgagcagttt tttgcccaca cctttgttat
     7921 gccaaattgt taggctgtct gatttccatc cagccattct ttagccagcg atctacctca
     7981 tcaaaacaaa aaattttcct ctgatggttc cttaaataag actattttga tcctttttcc
     8041 atgaataaga ttttgcatca gacaatactt catttctgaa aaagttgcgg caccaatata
     8101 gcctaatacc cctttccgat cttgattaaa tacaaaaaat aaatccgctg ctgcgatatt
     8161 taaaaaaaag tcgtcaagat ttctggatac tctttgtcta ggatcggaca tgagcgagga
     8221 taatcaatta attcaatgcc ttttttactg aaatcttttt ccatatctcg atattcttct
     8281 tggaaaacaa tacttccaga aataacagct ttcacaaaat tggcctccat ttttttattt
     8341 ttcagacact ccaccatacc ggatacctct caaagaaaag acatagcaaa aatcaatagc
     8401 agctatctct ccacggtttt ttactggtaa ttttattttt cttttggaaa ttgtatcagg
     8461 aaggtcgttc cttcattttt tcggctggtc acttcaattg aaccgtggtg caggcgcact
     8521 agttccttca cgattgataa tccaagacct gactcgccta tctttgtatt tttacttgac
     8581 ggatcggctt tataatagcg atcccaaatg tttttttgtt cctcttcaat catgccgatg
     8641 ccattgtcct cgacagtaat aaatacaggc tctttgtcaa atgatgttgg taaagagaaa
     8701 tcttagaata atttggacag aacagaaaga agaaactctc tttctgttct gtttatttgg
     8761 ttaaaaatgt tttgccatga cgaatcatga tccgtagttt gaaattgtaa aagctgcgaa
     8821 agccgtacgc attgcgtttt aatactttga tgtggttgtt taaacattcc aatggtccat
     8881 ttgaatagct gtagtgaagc gcattttgga tctgtggcag aaacttttta aacgttcgta
     8941 aagtggttgt ataggtttca ggaagaccgc tgacttctct gtttaaacag tcctcaaata
     9001 atgactgatc tcttttcttt accgcataaa gaatgtcttg gtataactga tagccttggc
     9061 gcaggttggg acaagcagat aacagtcgat cgacaatctc actttcagtc aaataatctt
     9121 taaaactggc acgccaaacc cttttttcgt agtttaactt tgcttcttct ttttgtagga
     9181 gtttccaata gcgtttcaat tttttcccta accctcgttg agagtgggaa gttcctttaa
     9241 gccgtgccgt ggtttctttc actcgatgat tcaagaaggt tctgccaata tgttggacga
     9301 tgtggaaacg gtcgatgatc aactgagcat tcgggaaaag ctttttcacc aatgaaacat
     9361 aaggcgcata catatcaata acaatccatt gaacggcttc acgagtcgct aatgaaaacc
     9421 gagaaaaata gcattctaaa taaggcaagc gtcgattttc gacaatatcc aataatgcat
     9481 ggctttgtcc gtccatcatg ataaaactca ttgcactggc aacacttcga accgatttga
     9541 attcatcaaa gcataagacc tttggcaaag cctgtttgaa aggattcaat gggcggtaga
     9601 atcgcttcat gacacgatag accgaagaag tggaaatatt tttctgacga gcgatttccg
     9661 tcatggaagt attctctttt aagcgagcaa tgacagacca acgaactttt tgactaatga
     9721 aacagtgttt ttcagcaaca gaggtatccg ctacgaatgt tctttggcaa gacttacatt
     9781 taaatcgttg tttcttcaac tccagataag tgcgatactc agacgcatca ttcagtaaca
     9841 accgaacggt tgtcgtcccc caacgaatga tcctagaggg gacgacacaa agacaatggg
     9901 aacactcttc tggcttgtac agaagagttc ctttgtaaat catggatttg agtcaatatc
     9961 ttggacacat tttagtagag acggttaagc cattttttct tttgctaatt cttcaaattc
    10021 ctgtggggtt agatagtgaa tcgagctatg gattcgattt cggttataaa atccttcaat
    10081 gtagctaaat agcgctcgat ttgcttcttc aaaatctgag tagctagtcg tgtagacttc
    10141 ttcttttttc aatgaggcat ggaaagattc gattccagca ttatcataag gtgttccttt
    10201 tctactataa gaatgcctta ttttgttggt tttaagccat tgttccactt ctcttgctgt
    10261 gtattggctg cccaagtcag tgtgtagaat cattccttct ggaatgtgat aatgtatttt
    10321 tgctttattc aatgtttgaa tgacacaatc cacagtcatt cgttttgaaa atgtataact
    10381 aataattttt ttcgtgtgta aatccatgat tgaagataag taacaccaac catttttctt
    10441 agtaggaatg tacgtaatgt cagctgccca tttctcacag atcgtttcag tagaaaagtc
    10501 ctgatttaag agattctctt ttgaaatgat cggtttatta gacctttgag gtctatattt
    10561 tttaactaca atcgacctta aatttaattg tttcattagc ttttggacat gtttaagaga
    10621 tactgaaatc ccttctttta ataaaacttg atggatcttt gtggcaccat agcgttgttt
    10681 attcgtaaaa aaaattgttg aaatcttctt tttcaactta ttatttctga tttctgtagc
    10741 tgtcaacggt ttattcttat aaaaataata gacacttcta gggatttcta ataaccgaca
    10801 taacaaagaa acagtgtaat ccttacacca tttttcaata aattgaacaa gcgaatttag
    10861 gttttccgag agaatatggc tgcggctttt tttaagattt caagttcctc atttaaacga
    10921 gcattttctt ttctcagttc agctacttct tttccagtca gtcctgtgga ctgatcaggt
    10981 aaatataaat tcttccattt gtaaatcgtt gcttcagata agccatattc tttcgctaac
    11041 cctcgaacag aacgtccagt ttgattcagt tcaacaatca tttgtttaaa attttcttca
    11101 tatcgggtca taaaaattca tcctctcgta gataagttta atttattctt gtctctacga
    11161 aaagctgtct aagatttgat actaacacca agttatcttt agaaccattt cagatacata
    11221 tgaaacaatt acatatgatt ttttcataaa aaaacagtac tgtctagtag tataaaatat
    11281 acaaatttct taaaaatttt ttcactaaaa tcactacctg aaacagtcta aaacacttat
    11341 atatcaacgt ttaatgaaac tatctccgtt tcatttcaga tacaaataaa tatagtcggg
    11401 ctccaaatcc gaaaaaaaat acccccagta gcttatccta agctactgga gatgtataag
    11461 tacggtcata taacaacggg gtagggtaaa taaatgaaaa agatggaaga atcattattc
    11521 aataactttt ttaaatactt cgttgtaatc agttacctgt ccgttcttaa ataccacaga
    11581 ataatctttt ttcccatttt caagtaaccg aagtggaatg tctttaccaa cttgctctgt
    11641 cactgcatta ctgatacctg ttagattagc taataaatct gataaagatt gagcatctgt
    11701 agaaatctct gtatcagtat ttatcgtgat tcctttatct tcatttacaa gcttccaagt
    11761 aggtggtata gctgtgtatg tttcgatgaa tgaatcaatt ctttcatcta tcgtcttccc
    11821 agtatctact tctgtctcat tactcttaga tgaatcgatt ctttcatcca tcgtctgctc
    11881 cgtaactgct tctgtctcat tattctcacc ggaaactctc ttcacaccca aacttatata
    11941 ttgtccatct ttagtgtgca tagttaaaat tcccacgctt ttactaataa attcactata
    12001 accagttctt tcaatagaga cttcaatgta tttcacacta tcatactcaa aagaaaaata
    12061 gacatgtaaa agcattttgt tgtttacatc tacataataa tcctttgact ctacttgaga
    12121 ccttcctata cggaatacag ctttactgtc tgatatctct ataccagcat ccttattatt
    12181 aatctgccaa tctccaagta gctcttctgg caagctagta gtttcttgtg aacactttac
    12241 cacttcactg gtagaggtcg aaacattcac ctccgcactg ttagaagaat tttccaagga
    12301 atcaggagcc ttagcacaac caacaagtct ttatatattg tgcagccgat attttcttca
    12361 ttcagtacac ccttgaaata tgcagtgtta gataaagcag ttcgtcctga tttgctgagg
    12421 cacccatctc ttcttgaacg tactccttga ttttcaaggc cgcccgatac tctttcggaa
    12481 aacgctgctt cactgaagcc agcaaaaaat catccttctc cggcaactct tccttcttaa
    12541 tcattcgggc aataaaaaac tgtaaatgcg tcacaaatct taagtaaagc atcgaatctt
    12601 tatcaacctt tttttcggga aacagatatc ctagcatagc caagatatcc tttagtgctc
    12661 ttaccgagag catggtttct tccatatttg cttgttcact ttgggcattt acaatgtgaa
    12721 acgcaatgtt tccagcctcc tcttctggca actgaacatt catttttttg ttgatcaatt
    12781 ggatagcata gcagccgatc tcaaattctt ttgggtaata cttttgaatt tcaatcagca
    12841 ggcgattttg aaaaacaatg cctttttgac tgcgttcaat ggcatgcttc aagtgatcaa
    12901 ccaaggtaaa aaatagactt ttattcagct taaagtcaaa cttggcgttg gcataatcga
    12961 taattcgctt gacgcaaata attagatcat ccggagcagc ctcaaacagt ttaatgtaat
    13021 ccttttttag agcatcagaa agtgaatcta atttaaatac tttttggatt ttgtcagccg
    13081 gaaccaaatc gcctttcttt agttgaaatc caatcccttt ccccatcaaa attacttctt
    13141 tgccttgctt atctaagcaa agaattaaac tgttgtttaa cgttttaatt actctcatta
    13201 gattcaccgt tctctctaag ctaagccatt tcttgcgatt actttttgat accaaaagaa
    13261 gctttctttt tttattcgtt ttaattcttt aagattctca tcggttcggt caacatatat
    13321 aaaaccataa cgcttattga agccttgatg ggtactaaca acatcaatcg ctgaccaagg
    13381 taaataacca aaaacatcta caccatctgt taaagctgct tgaatagcca gaatatgttt
    13441 ggctagataa tcaattcgat cctggtcatt aattcgtcta tcttcctcaa ctatgtcggg
    13501 tgccccatag ccattttcag taattaaaat aggtaaatca tagcgatcgt acaatttcct
    13561 caatgttaat cgtagtccag tagggtcaac tacccagcca tatggcgttt tatctacata
    13621 aggattttca gctgcccggt agacaccttc ttctcccaac atgatctgtt gatcccccgc
    13681 tctagcggaa acatctgatc cgtcattttt gctggcagcg atggtggcag ttgaataata
    13741 attaatcgca ataaaatcgg gtttaccgga ggataaaatt gcctgatcct ctgcctcaat
    13801 tttaggatat aatccacgat cattcaaata gttttcaaac aaataattgt attttccccg
    13861 aactgctaca tctaagaaag accagcctct caatgtttcc caattatggg ctgcaattgc
    13921 atcttctgga ctggaggtag cttgatacat agccgttaaa ttgattgctg gaccgatttt
    13981 tcctttaaga ttcaactgat gatacaagtg aattactcta gcttgagcta ataaaacatg
    14041 gtgattaatt tgaaagagtt ctttttttga aggcagctct ccacttggtg gcaaaccaat
    14101 cgctccagga tgaaggatca tcgtgttttg ctcattaatt gtcagccaat agttcacctt
    14161 gtcaccatac tcagtaagca atacctgtac atagtccaca aaataatcga tagttgctcg
    14221 atttaaccaa ccgccctgct cctctagaaa ataaggtaaa tcgaaatggt acatggtgac
    14281 aatcggttcg attccctgat tcaggcattc atcaattact tgatgataaa aagaaattcc
    14341 cttttgattg gttttattat tttcaatatc cataattcgc gtccaagcga tggaaaagcg
    14401 aaaagctttt aagcctaatt ctttaaacag ctgtatatct tctttatagt gatggtaaaa
    14461 atccgaggca acttcaaaat tagctacccc ttctgtatgc tgatacttgt caataataga
    14521 cggttgtttc ccatcctcct gataggcacc ctctgcctgg taggcactaa tggaggcacc
    14581 ccataaaaac ttctctggaa atgctgaaag ctgtttgtat tgcacaaaaa tcctctcctt
    14641 aactaatttg catcaatggt tcgtgttcct ttaagcttcc gcttgcttca ctaattcctg
    14701 cgtaattttc actgttggtc actaccacaa tcactgtatc atcgtagcca gcgtttctaa
    14761 tggcagcgaa atcggcgttg attaataatt gtcctgctga cacctgatct cctgctttta
    14821 caaatacttc aaagggttcc ccttttaaat tcacggtgtc gatccccaca tggatcaata
    14881 attcaatacc ttctttagaa cgtagaccaa tcgcatgttt agattctaaa atagtaatga
    14941 cttctccctc aacaggtgaa taaatgctgt tttcttctgg atgaatggct acgccctttc
    15001 ccaatagtcc cttagaaaag acagcatcgt tcacttcaga aataggacac acggttcctt
    15061 ttaccggatt ttcaacaatg actgcttcgg ctattgccgt ttgctctgat tcatcagaag
    15121 tatcgtcaaa tccaattaga taagcaatga taaaggttgc aacaaaggcg attacacacg
    15181 taataattgc gtgaacaata ttcattggat tttctccaat aaaggctggt aaggctgcta
    15241 agccagggga aacaaaggca tatctgacta aaccgctcaa gccagcatac aaaccagcaa
    15301 ttgcaccacc aatcatggaa gcaactaatg gttttttcag cttcaaggtt actccataga
    15361 gtgaaggctc agtaattccc attaaagcag tgatcccagt ggacacagct agctgcttca
    15421 actctttgtt tttagttttc gcggctacag caaaggtagc acctgattga gcaatattag
    15481 aagccagcat tcctggccca ttgatcattt cagaacccgt actgctcaat tgcatagtag
    15541 cgattggcgt cattgcccaa gctgtacccg taaccaccaa aaatggctgc aaaccaccca
    15601 tcaaaaatgg aattaaccaa gctgctttgc cgttaatcca ttcagccccc attgctataa
    15661 gatcgttcag caacgttcca aacggtccaa tgacaaccaa cgctaaaggc cccataatta
    15721 aaaataccag catcggctta gtaaagaatt taataaacga aggcgaaatt cgatccgcaa
    15781 agggctctac ataggacatg atccaaacag taaagataat cggtaaaact gatcccgcat
    15841 aatctgcagc taataccgga attcctagaa aagaaacggc atcgccagta gaaaaaagct
    15901 gtgaaatatt tggatgaagc aaaatgccag caacagtaat cgccaaaatt gggttgacat
    15961 caaactttat ggctgcccca taggctaata gtactggcat aaaataaaag gctgcatcag
    16021 caatagtatt caaaatgatg taagtttgac tactgtctga tagtacacca gtcaaagtca
    16081 agacggccaa aatggctttt accatcccgg cacctgttat agctggaatc atcggtgtaa
    16141 aggtggtaga aatgacacta atcaatcttg aaataatact ttcttgattg tctgaagagg
    16201 ggcccttttt aattgaagtt cccacttcct tttgaatttc cttgaaggct gtttggactt
    16261 cattgccaat caccacttgg tattgtccac cgctatccac cacactaatt acaccaagtg
    16321 ttttttctaa ggctttcgta tcggctttcc ttttatcttt gagttcaaaa cgcagtctgg
    16381 tggcacaatg ggttaagctg accacatttt cttttccccc aacctgttgc aatacatttc
    16441 ttcctacttc tctgtaatcc attattctct ctccttaaag cagtaaaaaa gaccaagtca
    16501 aaccacgccc caatcaacat tgaagtgtta gtttgtcttg gtcttgcctg cataatcagt
    16561 aacatgccaa taacgttatt ttgctgtaat ccaataatag cacattatga tagcgcaatc
    16621 aacagggaat ttaatttttt atcttttcgt ttgaccgtat agggtaatat aacagttaga
    16681 tcacatcaac tagggtatat ctgcgtatac ttactgctaa aataagtata tctaatatta
    16741 actataccag taaaaccatt cctacataac cagcgggaaa ttcccaaaat cgtgaaagca
    16801 tcaaaaatgc atataacgga tcacacaact tcaacaattc tgctcttggg aaaaattgat
    16861 gaatttggtg agtaaaggat ggaataaaca gcattccaac aatataggca acaatagtga
    16921 tacttgtttt tttggtaaaa atcagtaagc aatagccaat ttcaatcatt ccgagatata
    16981 aaggtatcga tagaatgatt tgggctagta ttcttaaaaa ttaactagca aatgtttaaa
    17041 ctgatttatt tctaatctac atgtagtaag tatttcaatt ccaccaatta tcaataaaaa
    17101 taccaaacat atccataata attttttctt tttcatttct tttatattgt tataacagtg
    17161 aataacataa aaattcccga caatcattgt aatatttttt tgtcacctgt attagtattt
    17221 aatttatagg aaactataaa aactccctag tgaatttaca cctttgggag ttcctgtttg
    17281 gacggtatgc ataatacatt taaatcaatt taacattaac acctcagcat atggtatctc
    17341 cacatatata cggtctttct ttggatagta ctcaatattt atgttgatag cacttccatt
    17401 gtctaatata gctttagccc ggtatgtgtt atctccattt tcatgagtaa tcgtcacatc
    17461 atcacatgat cgatttaagt tgtactgttc ttgtaaaatc tgtgttacca atggtttcga
    17521 tgacttctct aatccagatg tacctgtcat ttttgaaaaa atgataccta taatagccaa
    17581 gacaacgata actataatga taaacttctt gttcatattt actttattca actttttatc
    17641 tcctatctcc aaaataattt gctcttatcc ctcttatcag tacattgaca tccctaaaag
    17701 tacaccaaag attgctccag ctacccctgc tatcatcaaa atcgtaccca tttgttcatc
    17761 tttatctcga tataagtagc ccataataac agcaacagca ccaaatacaa caggaataaa
    17821 tgctaacgct actaccatag aaatccaacc aataatcaaa tacagcttcc ctgtctcttg
    17881 tccatgtacg atttgttcct tcatttcatt aattgtctgc tcagtacttt tctttactgc
    17941 gtctgctcca gttgtctcat ctgtttcttg tagtgctccg caaaactggc aatacttact
    18001 ttcatctgtt atttctttgc cacattttct acaatacatg tcttctcctc ctaaaaacat
    18061 tcaattgttt attagttaac tgtttacttt attaaataca tcagaaaaca gctcattcat
    18121 tggtacactt aatatctcag aaagcatctt catttgatag ggtttaggaa tcgttctccc
    18181 tgtttgccac gaacttacac attgctgtgt aacattcatt tttttgctaa ctccgtttga
    18241 gaaagtcctt tttcttttct gtatcggctg agattactta cttttttcat gattctcccc
    18301 tcctgtttta ttatatacaa caatatgatg tatttcaact cataaaatat aaaaaaactt
    18361 caaataccat tacaaataca acaacttgtt gtactatact gagatggggg tgtattaatg
    18421 aaacagaaac aatatgcaaa ggtcattaga gaaaagcgaa aagctctcgg gtggacacaa
    18481 aaagaattag ctgaaaaaat cttctctaca caacaggcag tagcaagatg ggaaaattct
    18541 gtaacagagc ctaacctcga tagcttaacc gctctttcta gagccctagg aacccctgta
    18601 agccattttc tagacaatgt tgtcgtagat tatgaggaag agtttttggc tctctatcgt
    18661 tctcttagca ctgaagatgc agttcgaaca attgactata tgaagctatt aaaaaggcaa
    18721 gaaaatgaac gcaatcagct attgaaagat tgaaaaataa ataacccacc gaactttaac
    18781 ggtgggtgta ttgagatgaa ataagattaa gagctatgga taacctgtct agtttaatac
    18841 aattgggtct tttctcttcc ttactctccg tccatcatct cttgttacac ttgtaaattg
    18901 ataaagagga actaattcaa ctcctgataa tatcaattct tttattactt catctatccc
    18961 tgctcctttt gctaggtaga ctaaaaaact actacttgct ttcacattgt atagtctctt
    19021 aatatcctta gaagctcgtt caagagtgaa tccttgttta acaaagtagc ctctgttttc
    19081 ttttaaagca ttttccacgc tgttattccc aacattacgt agacgttcac aaacagtgtt
    19141 agtaagttct ttctctgtca ttagatatgt tccttgccac gataagccct gtaaaacttc
    19201 atgtgcttca tctaaaatca attgtttttc cttgctaagt tctgatcggg aattttgagg
    19261 aaatactctt ttagcctctt tttcgttaaa acatatctta acccattcat aagaaaaata
    19321 ttgatatgta aaatgaaatt tttccagaag tggaaggata tcttcattca atttttgaat
    19381 cacagaagtt gcattaaaaa cgtctaatcg atagacattg cttctcttat gattaaagtt
    19441 tcctaacgct ttctcttcct ttaacctatt taggtattcg agcatcttca aaggaacatc
    19501 ttcatcatct aattttttta tcaatcctat aaaagtcata agtgatagta acttattaca
    19561 tttatctatg tcataagttt taccatagta aaaactcatt tttttagcta attgttcccc
    19621 cgacaaaata caagtcacac tttccttact atcatcaaaa aaattaacat cagaaagcaa
    19681 gacatcatag acacgtcgca tttcctctag ataattcctt attgtcctga aaagtttgtc
    19741 tttcattgat tcaagagtgt caaaaagtac ttttgattcc tctttcaatt ttcgaaaatg
    19801 tgtaggatac aagtctaggt tctctaatag ccaattaaat gtttctaccc gactgtcttt
    19861 ctttaaaatt agcatcacaa ctccaataaa attttcactg atatcacaat tactggaatg
    19921 acaataaaaa atggaatgcc cagttttact ggtaaaaata ctagcagagg gattatgatc
    19981 gttgtgaaac agacatctta agtttttact tgacttaagt aatttattca tatcaatggt
    20041 taacaatctt tcataaatct cattaaaaga catgtcggag gaatcaaaca ggcagtcttt
    20101 aaaccactgt ttcatttctt ctttgtcgtc attttttatc aacttaacaa aatcggtttc
    20161 attgactagt tcatactctc tccctgaatc actttgacaa tttttagaac atgtcatggt
    20221 aaaatcaatc ggaagctcag taatatctaa catgttgtct tcattaatta ttatcactgc
    20281 atctgactta tatcctccaa aaaacattct attcgaagca tttacattgt catcacaccc
    20341 ggggatgttc tttttcaagt attcgtaaac agcaaccatt ttaccatagt cagtaattgc
    20401 ttcttttaat agaaagacaa tcttgtattt ttctagattt tcagagtacc gaatactttt
    20461 ttgtatcgca aacgcagaat tatgaactaa attgatagat aaaacctttt ctatgcttaa
    20521 gtatctacta tcatctttac tcaaatctac taccttcccc ttctcatctt taacagtgtt
    20581 atcgacatcc agaataatta ctctggtttc cacaaaactc tccttgtcat tgcctttatt
    20641 ctttgtcttg cttggaataa atgattgtga tgaatttagt atctctacaa actcatctct
    20701 tgtaacttct ctatagttat ctaaacacct atctcttaca tcagtttttt ctttttttgt
    20761 aactggcttc tctaataatt tttctttatc tacataaatt ggaatattaa tcattgatgt
    20821 ttccatttgt gtttcctcct tataaaattc tgttaaagac taaaacatac agataataaa
    20881 aacaaactct ttattactat atgttttagt cttatttgtt tactcaacct ttataccgtt
    20941 ccatgaaact atcaatatca gattttttaa atctaatgac cgtatcaatt tttataacag
    21001 gtaacccgta ttgagaaaca tattttctaa tcgttcctac tgatacacct agatattcac
    21061 ttgcctgctg atagtccata tactctagag tgactccaga aataatgtct tctagcatag
    21121 gctttagcac tgtaattatc tctttcactt gttgttcaac ttgtatagaa acaatgctat
    21181 caatgatttg tagagacata tttccccctc ctttcgtgat tgcttgaaca aattttctaa
    21241 gaattatctt cgtatttttt gctcaaaata aaagtatttt gttattaacc gattaatcaa
    21301 acaaaaaatg attaccagtt catcgacata cacataatac ctcgataaaa aagaggtgtc
    21361 aatatctttg taaaatttat ttacaacacc cctttttcga ggtatactaa ttttaggagg
    21421 tgtggtcaat atgataaaac gtccaccaat aaattattta gaacgaaaaa agatactcgg
    21481 aacaaaaata aaagctataa gaaagagtaa aaaattaaca caaccagcat ttggtcttat
    21541 gattaacaac ggtcaattaa ttgacaaaaa aactatctac gaatgggaga agggaaccta
    21601 tttaccaata ccagagcgat tatcccgaat tgctgatttg ggaaatatga gtatagagga
    21661 gttagtttgt ggcaatgtag aagaatatat tttgggaatt attctttata gagatagcat
    21721 tgtactagat ggtataacat ttccagataa aaacctgttc cagcatcttc gtcaacagtt
    21781 cccaccagta cactctaatt tagatacatg gctggataga tattccaaat tagaaccaga
    21841 gatgcaagaa tttattgcta acaagacttg taataaagtt aaaaatgaaa aaattagtct
    21901 tttcaatatc ttaaaaatag aagaattatt catcaatgct attgtagaag aatttgacaa
    21961 taacattctt ttcctcacta gcagtataga agaactacta gaaagaatgg tagatgaatg
    22021 gctacctatt caattgaaag acatgtctta tcccgaagaa gctgtgagag aaattactga
    22081 taatataaac aagctagaac aaacgatttc atcaattggg aaaaaataca ctaagaaaaa
    22141 aatgaaggga ggtgatacca tctagtggta agtactcctt gtaaactaag aggagatgat
    22201 ttcatggcaa aaatatcaaa gtacaaaaag aaaaacggtc aagtggcgtg gatgtataaa
    22261 ggacacattg ccaccgatcc acgaactggt gaaaaaataa atactacgag aagaggtttt
    22321 agtagcaaac aagaagctca aaaagattat gacgagtaca gacatcaaat attatacggt
    22381 gttaaaaaga aagcacctga tatgacattt gaagacctgt ataatgaatg gataactcat
    22441 cagcgtacca gtgttaaagc ttcaacaatc gcaatctcag taagatacgc aaataatcaa
    22501 atacttccag catttggtaa gcttaaacta tcgaatatct ctgtcccata ttgtcaaaaa
    22561 gtggtagatg aatggcacag taaatatgaa agttacgatt atatgagaaa acagactgca
    22621 caaattttac gctacggggt tgcaatgcaa tatattgaca ataatcctat ggaaaaaaca
    22681 cttcttccac gaaaaaaaga gtatgagaaa aatcgtaaat tttactcaaa agaggagctt
    22741 aataatttac tggatgcttt taaagatttt ggtaacatga aacaatacgc cttcttcaga
    22801 ttattggcat acacaggtat gagaaagagt gaagtactcg ctttacagtg gaaagacata
    22861 gacacgttta ataaagaact acatgtcaat aaaactcttg ctgtagatga atttggtaaa
    22921 gttataattc aatctccgaa aacaagagct tctagacgag ttatctcttt agatactgag
    22981 actctttcta tcctcaacaa ctggaaatta cagcaaaaag aagagtattt aaaacttgga
    23041 tacaatacta gctctaaaga gcaacatgtt tttacaacag ttaaaaacac tctatacatt
    23101 ccaaacactg ttaatgattg gttaagatat attttgaaaa aatataactt gcctcgtatt
    23161 acacctcatg ggtttagaca tacacatgct agtttattat tagaagcagg agaatctgtc
    23221 aaggtagttc aacaacgatt agggcatgaa aatagtaaag tgacattaga catctatgca
    23281 catattacaa ataatgcacc taagaaaaca ggtcaagact ttgctgatat gatggctcat
    23341 cagtaaaaga gctggtcaaa atctggtcat ttctagtata caggcaaaac aaaaaagcta
    23401 gaacccttat atat
//
LOCUS       DAZKLM010000042        22973 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3764
            SAMEA115706913-rid22582563.denovo.042, whole genome shotgun
            sequence.
ACCESSION   DAZKLM010000042 DAZKLM010000000
VERSION     DAZKLM010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706913
            Sequence Read Archive: ERR13196647
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22973)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22973)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287823.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287823.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,004
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,849
            CDSs (with protein)               :: 2,849
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 41 of 96
            Pseudo Genes (incomplete)         :: 52 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 15 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22973
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706913-rid22582563.denovo.042"
                     /isolate="VRE3764"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..1835)
                     /locus_tag="ACG842_001475"
     CDS             complement(<1..1835)
                     /locus_tag="ACG842_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail spike protein"
                     /protein_id="HFQ0248023.1"
                     /translation="MTQEFIYAYKKMPDDLSVNGASLVDWEDLPEINRVLNGQYRFYG
                     NYSRSGQYRSYLKKGNFIKAKVPDGSWQYFEIYNVKKNLTSVSVTARHIGFMANKNFI
                     VKSFTDNGNGSQIMTNLKNSLAFDQKFNYLSNVGTTHQFTARQVAPVEAIIGSNNGNQ
                     NLTGVTSAELDMDNYDLKLVKQIGSDNGFRIDFGINLEAIEEEIDEESIVNSLYLVGG
                     VPDNDYDEDKEPIEYGYLEIDGVTNENRRIAKRENGDCKTVDELIKWGKTLFDNDRIH
                     EPKATHTVSMVALEHTLEYGDMYQELASLHFGDVAHVRAKELDIEIKERMVEYTYFPT
                     LGKYKDIVLGNDLSLYTSAVNTQAQELKKKIDNRTETLVQNVLNATAWITGNSGGHVV
                     FRPEKAPSEILIMDTPNVANAKRVWRWNLNGLGYSDNGVNGPFGIAITSKGEIVADFI
                     KVGTINAEVFETSFNAYGDVLKLVKGTLQIWNENKKIMELTKKGMEFWNRKESIGTIG
                     TTDSAGNPFPNAVTPTPLEENSLVIRTNGDGKYILISPTAEKGFVLLGNGKAYYFGDL
                     DIQGKLTVRGKEVIPGQNGGPSGGGETPGGYPDELKTDAEKRAWR"
     gene            complement(1832..2536)
                     /locus_tag="ACG842_001476"
     CDS             complement(1832..2536)
                     /locus_tag="ACG842_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFQ0248024.1"
                     /translation="MNPDEPNFIWKNRNAVIDMNCIIEAELPDIMPNKRYETYTIQGR
                     SGELTETFDDYEPFDLEIEGITIPHSKLREVKRWLTGKSQLITHNDPDKYLEAICSMD
                     KEVPFENEWGFFYTFDVTFRCQPLKRKIGEAPKIFHSSVMDIFDHGDEIAHPYLEIES
                     NGGDITIAVGDKSLTILNTLAATIVVDTQLGKAIQEGLNLFTRGDWPVLQPEWNQVKI
                     SGKFKEVRFWNRSVYL"
     gene            complement(2548..4857)
                     /locus_tag="ACG842_001477"
     CDS             complement(2548..4857)
                     /locus_tag="ACG842_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFQ0248025.1"
                     /translation="MAGKESDVVLNFKTNGEVSYSKTIKEINKEMNLAAAEYKNQVSA
                     MDKDATQTEKLRAAKQKLEKQLGLAEQRSQMLREEYEKSVKETGEYSAESEKLYKQLL
                     NSETGENKLRTALEQTNDALKEQGDVSVDTAKKLQKIEETGEKVKGVGEKMSVGVTAP
                     IVAAGAAGLAAFGEVDEALDTIITKTGATGDQADRLSQSFKNVGSNTHLPLQTVGEAI
                     GEVNTQFGFMDKKLEDSTNYLLQYAEINDTDVSQSAISARQAIDAYGLEYDDLNSVLD
                     VTTKTSQNTGQSVDDLMQKAIDGAPQIKQLGLSFGEGVTLLGQFEQSGVDSSAALSSL
                     SKATVAYAKDGKSLSQGLEELQDKVKNAGSETEAINAAAEVFGTKGGPRMADAIRRGT
                     LNLEDLAKTAGESGGAVGDTFDATLDPIDQADQAMNNAKLAMADVGEAVQVSLLPFFE
                     KATSLLQEFSKWWGSLDQDTKNWIITIAGIAAAVGPVLIVLGSLMGSVTKIVGEVKSF
                     IGVWQGLAGLFGMSGGWFALAVIAIGALVTGLIWAYNNVKWFHDGVNSFFKGVSDVAV
                     EIFNFLSGYFGGFFDGVIANFNNFYNAGKRIFSGFLDFITGVFTGNWSKAWQGLVDIF
                     GGIFDGIVAVGKAPINAMIGLINGFIGGLNNIKIPKWVPGVGGKSFSISKLPYLAQGG
                     HLINGQAIVGEAGPELLTAKNGKTTVTPLSDEEKRKGISGRVSGGSIEQHIHIGKVDA
                     NNPSELDRMNRKFAKANKQAIRDLGGVPI"
     gene            complement(5052..5516)
                     /locus_tag="ACG842_001478"
     CDS             complement(5052..5516)
                     /locus_tag="ACG842_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0248026.1"
                     /translation="MARLSDYGIHVEDLKNSAVVIIQGAEFPISFTMQTMEFIADVYG
                     GDYSQFESDMNAMLSKKEGQISSANLSPSDLKIMRALIYAMLRTGGLEEDPETIFKFL
                     GMSGEVLSAYSTCMEIFASQTFQVEDLKKSKKPQDFQKAQAKRKKNKKNRKR"
     gene            complement(5516..6142)
                     /locus_tag="ACG842_001479"
     CDS             complement(5516..6142)
                     /locus_tag="ACG842_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFQ0248027.1"
                     /translation="MGKQDTFYFEGLDDILIAMMATPDSVGTAPTYSEVLRLPIATKI
                     GVKGNGTALEKWASSKMFRRVSRETKHELALDHVGIPIEVMDEIKGLVAKSGVTFSKN
                     TAREFPYFAFGFIGNIENGGKKAVWYPNTQLSNVIDEEYATAEEETKIDDVTANLVSI
                     GLKYNNVMYASFDSNRDGASLELFNKFIAQPVYDEEQWKTLAKVGGAG"
     gene            complement(6149..6514)
                     /locus_tag="ACG842_001480"
     CDS             complement(6149..6514)
                     /locus_tag="ACG842_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97-gp10 family putative phage morphogenesis
                     protein"
                     /protein_id="HFQ0248028.1"
                     /translation="MPSNKNGFSEISDYLGNLSKVDPKKLSLESLEEAANFYLKQLLP
                     NIPKSLLKKKHMRDQIKVVVENDQVKVQFEETAFYWRFAENGTTNQRAQHFASGTYEQ
                     NKEKIEEIMTRKILDLWEG"
     gene            complement(6504..6842)
                     /locus_tag="ACG842_001481"
     CDS             complement(6504..6842)
                     /locus_tag="ACG842_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0248029.1"
                     /translation="MSISFDELKTALKSTKLAVFRDKARLGTMYPYIVYSNVSNSKKM
                     ASGKVYKKLPYYQVSFFTLGTEQDLVVIEQALQNAGIPYSDFTGIQGDENDDTVTNYY
                     TYVRCVENAK"
     gene            complement(6832..7158)
                     /locus_tag="ACG842_001482"
     CDS             complement(6832..7158)
                     /locus_tag="ACG842_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HFQ0248030.1"
                     /translation="MPKRQTNNLRWKAELLDIKTGTDGNDRPTTIYEFKRLIFYEELG
                     VTSQEKYLSQQAKTDVVRRIKVRWDKSITEKLSALKIDSVTYNITRIYTNPDAREMEL
                     SLAYVD"
     gene            complement(7136..7417)
                     /locus_tag="ACG842_001483"
     CDS             complement(7136..7417)
                     /locus_tag="ACG842_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002395304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail connector protein"
                     /protein_id="HFQ0248031.1"
                     /translation="MEEQTKELSLEEKFKSHIHFEEGMDDSLLSFYLNMAKDYVKTAT
                     GGQQEYLILMVAGIAYEYRVSEDELDKAMNAMTPFIVQGAIQNAEETDQ"
     gene            complement(7419..8783)
                     /locus_tag="ACG842_001484"
     CDS             complement(7419..8783)
                     /locus_tag="ACG842_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016622740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HFQ0248032.1"
                     /translation="MDKELLKKMKARREQRLTELREKVESGELREADLEAVKEEIDSV
                     IDELNGIKDELGADSGTDETDDNTDDQSNSTDSDESRSGEDNDQEEDSDSEDSSENRS
                     GMITQQQRDGLLGSIKNGLEARAKMTKKQKDQQLRKAFANFVAGNISEAEARALGIEA
                     GNGSVTVPEVIASEVITYAQEENLLRKYGTVVRTSGDVKYPILVKKADANVNKKERST
                     DIAETAIQFDEILLNPAEFDALATVTKKLLKMSGVPVEDIVVEELKKAYVRKEINYMF
                     NGDDAGNENPGALAKKAVAFKKPLDLTASGAGQKLYDALIEFKNTPVTEVMKKGRFII
                     NRAALTAIEKMKTDDGFPLLRPFTQAEGGIGYQLVGYPVDWTDAADKKGEPDTPVLYF
                     GDFSAFKIQEVIGALEIQKLVEKFSGKNQIGFQIYNLLDGQLVYSPFEPAVYRYEITK
                     PVGG"
     gene            complement(8796..9365)
                     /locus_tag="ACG842_001485"
     CDS             complement(8796..9365)
                     /locus_tag="ACG842_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA];
                     GO_process: GO:0019082 - viral protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HFQ0248033.1"
                     /translation="MKKKEIRTIDITNLSTRSDEETHTRTISGYAAVFNSQTLLWDDL
                     SEVIVPGAFSKAISNSDVRCLFNHDWSNVLGRTKSGTLRLEEDERGLKFEVDLPDTTV
                     ARDLVKSMERGDINQCSFGFVPTEETWDYNSEPMLRTIHEVELYEVSIVPLPAYEDTE
                     AALRSRDELEKNVEERKKLIKKINQALEA"
     gene            complement(9337..10524)
                     /locus_tag="ACG842_001486"
     CDS             complement(9337..10524)
                     /locus_tag="ACG842_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFQ0248034.1"
                     /translation="MEKRGYIEDIFGHYTRYGQRYVTDSSIMESSDVYELVQDISNQV
                     ALATPIVIGPDGNEVKDHHLLNILKSPNDYLTGFEFTKLETNTLLINGETFPLTDRDQ
                     LHLAYGVTTKINERLQEEFEMNGQKIPGQMIRHIKNIGTDSLKGAGIIDLARNTLEGV
                     LSAEKVLTDKYTKGGLLAFMLKLDAHINPNNSAQTKIVKAILDQLEGTQNESDHSVKM
                     IPLGKGYSIETLKSPVDDAAILNYLGVYKKDLGKFLGINVDTYQSLMKTDIEKAMMYL
                     HNKAIKPILKNKSEHYTALFFMPNSGYRVEWKINILDFVPYSTKTNIGYNIVRTGITS
                     PDNVAEMLGFPKQNTPETQAIYISNDLSRIGQKNATDDSLPTNDQDLKGGDGNEEEGN
                     SHD"
     gene            complement(10588..12315)
                     /locus_tag="ACG842_001487"
     CDS             complement(10588..12315)
                     /locus_tag="ACG842_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="terminase TerL endonuclease subunit"
                     /protein_id="HFQ0248035.1"
                     /translation="MIEPGVNYADLFAKEVRKKPKKYPKTVRLAIDRWYQWKKRKDIW
                     FDVDRANEMMDWVESFIVHTKGEMVGKPFLLEPWEKFIYSWIYGWVKENEKGQIVRVT
                     REAYVQIPKKNGKTLIAVGSLGYAMYGEGALSVDCYACASDFAQAQYAAKPFAVTILN
                     NPILLEGTKIFRGPKGTVSSITYDYIHEDMAYSNKFIVQTKNIDNIEGSNPYFVLNDE
                     LHKQEKMEQYDNFKSAQISLPQPLMFNISTAGKGSSSVGMRVYREAKEVLKRDDNDSN
                     FVLIYEPNKNYDWTDRKVWEMCNPNWGISVDLSALESAFKTAQRSAHSKAEFLTKHLD
                     VFVNGADNFFEQDQVEPCLVTTQELGDLSGEPCYIGLDLSRTRDLTCVSLNFPTWDED
                     GKAVLKVKQLYFIPNEDLEFREKEDNVPYSDLAEQGFVEFCDGKMIDQDQILQYIEDC
                     MNLYDIQQVNYDPAMSDKLVEKLENLGLECVEVAQYPKVLNAPFDDVERLFYEKRIQF
                     DNPLFLYCTLNVVAITNINGQKAPSKRQSKKKIDGFVAFLCGHKETMNQMTDIDSDEL
                     DDYLSSIYR"
     gene            complement(12312..12764)
                     /locus_tag="ACG842_001488"
     CDS             complement(12312..12764)
                     /locus_tag="ACG842_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P27 family phage terminase small subunit"
                     /protein_id="HFQ0248036.1"
                     /translation="MTTKAQRKAIIDEKVSAEKARILEIMNLSDLYTITLDPLIESYL
                     DIFEIYQHKYLLWKEKGFPETQKFTNKSGATNQSKHPLAQQVETWADKKMKALDLLGL
                     TNKAKTGRLIAGGSTARKDEEITRPEVKPVDELAAHRNKWRKKAGAEK"
     gene            complement(12876..13256)
                     /locus_tag="ACG842_001489"
     CDS             complement(12876..13256)
                     /locus_tag="ACG842_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HFQ0248037.1"
                     /translation="MKYCQFDGCTNKIAKGIYCTEHKRSSRSRKKKQQAKSVYHHENK
                     PFYRTQAWKDMRQFIYEREGGHCQRCGQFIFGKRAHVHHIVPIKDNELLKLDPNNLML
                     LCSKCHPIVENETEDKKVFPSYFN"
     gene            complement(13253..13639)
                     /locus_tag="ACG842_001490"
     CDS             complement(13253..13639)
                     /locus_tag="ACG842_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rho termination factor N-terminal
                     domain-containing protein"
                     /protein_id="HFQ0248038.1"
                     /translation="MAMIKIRTSISGTEYWDSEKKRTVVVPKDQEPNFEVKEEKGILS
                     DGKSFVAVNGELIAHSSEILDSDGNTAANFDGDEATNDQSTEDTDELDNMTAKELRAY
                     AKKHGIDIPGAIRAKGDIINLIREVE"
     gene            complement(13641..13919)
                     /locus_tag="ACG842_001491"
     CDS             complement(13641..13919)
                     /locus_tag="ACG842_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0248039.1"
                     /translation="MNDFHEAVLSIEVESSLAKVYKKAIEAENSPYRENWNGNHAHVQ
                     VENDDYRTGMNTLVISLLSHTLPNLQETIEWYERMGAKVIRTNYKGEN"
     gene            13967..14695
                     /locus_tag="ACG842_001492"
     CDS             13967..14695
                     /locus_tag="ACG842_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4145 domain-containing protein"
                     /protein_id="HFQ0248040.1"
                     /translation="MRGVLMLQEVNIKYRDRQNTETIYLPNSCPHCGNIMTPYVYFGV
                     SSYSAFDNDRIFGVLVQCINDECNKFYSLQFINTFNEDEGYSENILIKDYYRPPIKID
                     LPENIEKVSPTFVDIYTQATQAKSEQLEQIAGVGYRKALEFLIKDYAISKNSEDEDKI
                     KKMLLGKTIENYLNDFPKLQRLAKAATWIGNDETHYVRRHTNKDVHDMDAFIKSAAQF
                     IAADYDADLADQFINGNNFMPTTN"
     gene            complement(14841..15317)
                     /locus_tag="ACG842_001493"
     CDS             complement(14841..15317)
                     /locus_tag="ACG842_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HFQ0248041.1"
                     /translation="MRFHWLKDYQELDEQILYLKWNLNKSKLELNRWVCGDLANVRIE
                     KNSRTSSLEENIQKIENELELLIEQKEEMLLLIDSFSGIDNQIVKMKYVDQMSLEDIA
                     ESVGYSSSYVRQRHAEIRKTLNFLDEYEHRQADRLKKENEIDFYNSEKYKEQLSLF"
     gene            complement(15277..15633)
                     /locus_tag="ACG842_001494"
     CDS             complement(15277..15633)
                     /locus_tag="ACG842_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0248042.1"
                     /translation="MKKVNVIDLPVHFRGTNYFYASNLIQKKMSKDERKILANFYLVL
                     KIEQNVFSERRIVWEKQNQRLRRKNDVWNKRQSRMERLRKNKKATPFEIAAQYYSTIR
                     GGVCEISLVKRLPRTR"
     gene            complement(15611..15796)
                     /locus_tag="ACG842_001495"
     CDS             complement(15611..15796)
                     /locus_tag="ACG842_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0248043.1"
                     /translation="MKVYILAITEGTWMFPVGSGKIYKSKTAAYKAFEKYKKENGGGT
                     NAKILVADNWHEEGERN"
     gene            complement(15797..16069)
                     /locus_tag="ACG842_001496"
     CDS             complement(15797..16069)
                     /locus_tag="ACG842_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH/PUA domain-containing protein"
                     /protein_id="HFQ0248044.1"
                     /translation="MIHELKILPEYFEAVTSGRKQFEIRKNDRDFKVGDQLILREYKK
                     YIKHELPGLTRPTFSYTGDSYKAEITYITDYMQKSGYVVLGIKGVN"
     gene            complement(16066..16266)
                     /locus_tag="ACG842_001497"
     CDS             complement(16066..16266)
                     /locus_tag="ACG842_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0248045.1"
                     /translation="MKVREQFKIVSDEGNVYKIKIVNINEYREADHKYLVDLYMNDKY
                     LGEGFCGDSLFDDERILKEETE"
     gene            16394..16606
                     /locus_tag="ACG842_001498"
     CDS             16394..16606
                     /locus_tag="ACG842_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0248046.1"
                     /translation="MNINNLVKLVEQRFKESGLEQLKKDNSPELFSNFTEDDIPTLKQ
                     LKYYSDYWTQTRENELNLEKLIIKKK"
     gene            complement(16639..16833)
                     /locus_tag="ACG842_001499"
     CDS             complement(16639..16833)
                     /locus_tag="ACG842_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0248047.1"
                     /translation="MMIQLAGIQTGKIYFSGESKSEANQWLLKTYTNNKKLRKKYPDL
                     LLKDDQIMPEPMILTRKEGS"
     gene            complement(16846..17028)
                     /locus_tag="ACG842_001500"
     CDS             complement(16846..17028)
                     /locus_tag="ACG842_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0248048.1"
                     /translation="MRATDPVIILEEAKFIWTHEEIEQARLLFSQGVKPSKVAEIMGQ
                     KILDVGLLLLHLAEKI"
     gene            complement(17025..17165)
                     /locus_tag="ACG842_001501"
     CDS             complement(17025..17165)
                     /locus_tag="ACG842_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0248049.1"
                     /translation="MNEIAADHELSETALIDQIELARQYFVPYADTWSKHKNDIIFPE
                     EQ"
     gene            complement(17655..18020)
                     /locus_tag="ACG842_001502"
     CDS             complement(17655..18020)
                     /locus_tag="ACG842_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0248050.1"
                     /translation="MIDAKQVQKQKDGMLMFEAYVLPFLNQFEVLECSASGEELEYVV
                     IRETKENVQKLNEFLCTINCWDMIAPGFLCPAMGEFLEYCRLEDAGTLDLAYLVYNYL
                     NINTDHLWFGTAERKWVVR"
     gene            complement(18060..18362)
                     /locus_tag="ACG842_001503"
     CDS             complement(18060..18362)
                     /locus_tag="ACG842_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like family protein"
                     /protein_id="HFQ0248051.1"
                     /translation="MDELIAKVEQWAKDKGLDQADFSKQMLKTVEEIGEVAASLARKD
                     EHGLRDGIGDVVVTLIILAMQNDMDLYECLDFAYDEIKGRTGKMVNGVFVKSSDLK"
     gene            complement(18362..18661)
                     /locus_tag="ACG842_001504"
     CDS             complement(18362..18661)
                     /locus_tag="ACG842_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0248052.1"
                     /translation="MDKKAAMQRIIELTYSEDWQEDKKIVAEVQKLGKSMWTEKPKRR
                     TPRKIAIWHGDRILVTGTAKQLSEITGLSKNIIWDRARSLWIDSKGRQFRYVEEK"
     gene            complement(18654..18818)
                     /locus_tag="ACG842_001505"
     CDS             complement(18654..18818)
                     /locus_tag="ACG842_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0248053.1"
                     /translation="MDLKTFTAQIELMHQEALRQSVSYEDKWLNTFHGGRESALDQVL
                     KLLKGECRDG"
     gene            complement(18824..19093)
                     /locus_tag="ACG842_001506"
     CDS             complement(18824..19093)
                     /locus_tag="ACG842_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0248054.1"
                     /translation="MKIDYLELINEIANYKKGEELDVLRDVYDQLEEAGIEGIKNDRS
                     SWSKLRYYFALYIDGTQLRNLAYTKLLFIDCVKGLQKHLNELEQV"
     gene            complement(19105..19854)
                     /locus_tag="ACG842_001507"
     CDS             complement(19105..19854)
                     /locus_tag="ACG842_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0248055.1"
                     /translation="MTEHRSYYAIIPANVRYDKRLKPNTKLLYGEITALCNERGFCWA
                     GNEYFADLYGVNKETISRWVSDLIKFGYLNREIIYKEGTNQIINRYLRINQYPIDEKR
                     NTPIDEKVKDNNTSINNTFNNTKEYIRELPPSKKSKAKPVRHKYGEYKNVLLSDEQME
                     KLKTEFPNDYQERIERLSEYCESSGKTYKNYLATIRSWARKEKSEPKNASSGYKRTGR
                     REKLPEWAIDQEAYLKKKALERANRQSKAPF"
     gene            complement(19851..20543)
                     /locus_tag="ACG842_001508"
     CDS             complement(19851..20543)
                     /locus_tag="ACG842_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative HNHc nuclease"
                     /protein_id="HFQ0248056.1"
                     /translation="MFKPLIDSYSAVLKKFKGKDISATINEEVNIDRLKTMYDGYDGD
                     RVIEIRFIDPRRFTVQQRNFIYALIGDIFIDTGMPTDFWKEFFYFRFEGVTGRKISLK
                     DESNTTVSDANVLANIILDFIFEHHIPFKEGYEILPANQEYYFYKCITKRVCCICGKT
                     GADIDHFDKALGRRKRKEVDHAEYTFAALCRIHHTEKHKIGVINFKNKYQIKGIKLNQ
                     KTIKKLNIGGEK"
     gene            complement(20549..21220)
                     /locus_tag="ACG842_001509"
     CDS             complement(20549..21220)
                     /locus_tag="ACG842_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HFQ0248057.1"
                     /translation="MSEIKGTTNFEKLFSRKLNKILKKKGNFDYLSWAHAWEIMKKND
                     PQATVTINEYKHYRVVSGTHQDFLVEEYKPFLMDETGTYVSVSVTVKGHTETELFPVL
                     DYRNQPVVKPNAMQINNSLKRCFVKALALHGLGLYVFQGEDIPTPPRIDTKKLNMLET
                     ILEAFNEQMGKDMTKTLIEYVNEQTDKLGLLADNVETIEQLSYEQCALMERAIAAKRK
                     ELDKK"
     gene            complement(21213..21554)
                     /locus_tag="ACG842_001510"
     CDS             complement(21213..21554)
                     /locus_tag="ACG842_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0248058.1"
                     /translation="MEKELSTLDQYLIDPDWGKPKIEETSGRKIRRNLLTNEELAWDQ
                     DDLGNHVTIWDHVYLIHLSKHSNKPEYIYVIEDGLIDALEEYDRDNLIDISYYGPGKK
                     YIAEMEAEFDE"
     gene            complement(21732..22430)
                     /locus_tag="ACG842_001511"
     CDS             complement(21732..22430)
                     /locus_tag="ACG842_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage regulatory protein"
                     /protein_id="HFQ0248059.1"
                     /translation="MQELVILKNKEAVTTSLQVAESFEKKHQHVLRDIDALKKDVSNF
                     GQMFVEGNEPDSYGRNRRVFFISRDGFFLLAMGFTGKKAIYFKQKYIEAFNEMEDVIR
                     KNTVPQTIEDMMIYQLEEMKDVKKDVSMLKDTMRISGQQEFEIKQKGNMKVMGVLGGK
                     ESRAYEEISKKVFSKFWSEFKRTFSIPRYGELPRKRFDDAVSFIEMWLPETAIRMEID
                     QLNRQQRLFGDDNE"
     gene            22517..>22973
                     /locus_tag="ACG842_001512"
     CDS             22517..>22973
                     /locus_tag="ACG842_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0248060.1"
                     /translation="MSIISFILSTFLTGIKIKGMRPQLEVELNASYFAADMIYTKVII
                     SNYSTEPAMLVNLELSSANLNRRWSATPFKKLIAKGGSVDDNRIYSEAVPLNIPPKSA
                     ISCYLAFEVGKINFNKLLNHQTKMIFTLNRTQIHKIVDIKNTNFPIEKLV"
BASE COUNT         6388 a         4832 c         3560 g         8193 t
ORIGIN      
        1 attctccaag ctctcttttc tgcatctgtt tttaattcat caggataccc accaggtgtt
       61 tctcctccgc cagatggacc gccattttgt cctggtatca cttctttacc tctaacagta
      121 agttttccct gaatatctaa atcaccgaaa taatatgctt ttccgtttcc taataacaca
      181 aatccttttt ctgcagttgg agaaattaaa atgtattttc catcgccatt tgtacgtatt
      241 accaaagaat tttcctcaag aggtgtaggt gttacagcat ttggaaatgg atttcctgct
      301 gaatctgttg ttccgatcgt tccaatggat tcttttctat tccagaattc catacctttt
      361 ttggttagct ccatgatttt tttgttttca ttccaaattt gaagcgtgcc ttttacaagt
      421 tttaacacat caccataagc attaaaggaa gtttcgaata cttctgcatt tattgttcct
      481 actttgatga agtcagcaac aatttctcct tttgatgtga tagcaatacc gaacgggcca
      541 tttactccgt tgtctgaata acctaagcca tttaaattcc aacgccacac tcgctttgca
      601 ttcgccacat ttggagtatc catgataaga atctctgatg gagctttttc tggacgaaaa
      661 acgacatgcc caccagaatt accagtgatc catgccgtcg cattcaacac attttgaact
      721 aacgtttctg ttcgattgtc gattttcttt ttcaattctt gtgcttgagt atttactgcc
      781 gaagtgtaca gtgataaatc attacctaaa acaatatcct tatatttccc aagcgttggg
      841 aaataagtgt attcgaccat acgctctttt atctcgatat ctagctcttt cgccctaaca
      901 tgagcgacat caccaaagtg taaagacgct agctcttgat acatgtcacc gtattcgagt
      961 gtgtgttcca acgccaccat actgactgta tgagtagctt ttggctcatg gatacgatca
     1021 ttgtcaaata aggttttgcc ccatttgatc aattcatcaa ctgttttaca atctccgttt
     1081 tcacgtttgg caatacgtcg attctcgttg gttactccat caatttctaa ataaccatat
     1141 tcgataggtt ctttgtcttc atcataatcg ttatcaggaa caccgccaac caaatacaaa
     1201 ctattgacaa ttgattcttc atcaatttct tcttcgattg cttctaaatt gatgccaaaa
     1261 tcaatccgaa aaccattatc tgatccaatc tgttttacca gtttcaaatc atagttatcc
     1321 atatcaagtt ccgcgctagt cacgccagtc aaattttgat taccgttatt tgaaccaatg
     1381 attgcttcaa ctggcgcgac ttgtcttgct gtaaattggt gcgttgtccc tacattcgaa
     1441 agatagttaa atttttgatc aaatgccaaa ctgtttttaa gattggtcat gatctgagac
     1501 ccattgccgt tatccgtaaa cgacttgaca ataaagttct tattggccat aaaaccaata
     1561 tgccgagctg ttacagaaac ggaggtcaaa ttctttttga cattataaat ttcaaaatat
     1621 tgccatgatc catctggtac cttcgctttt ataaaatttc ctttttttaa ataagagcga
     1681 tactgtccgc ttcttgaata gttaccataa aaacgatatt ggccattcag cacacgattg
     1741 atttctggca aatcttccca atcaactaac gacgctccgt tcacgctcaa atcatctggc
     1801 atttttttat aagcataaat aaattcttgc gtcacagata cacactccta ttccaaaacc
     1861 gcacttcttt aaattttccg gatattttta cctgattcca ttcaggctgc aatactggcc
     1921 aatcaccacg cgtaaataaa ttcaaacctt cttgtattgc ttttccaagc tgagtatcaa
     1981 ccacgattgt tgcagctaac gtattaagta ttgttaggct tttatcaccg acagcgatcg
     2041 ttatatctcc tccgttcgat tctatttcca gatagggatg tgcaatttcg tcaccatgat
     2101 caaaaatatc cataacacta gaatgaaaaa ttttaggtgc ttcaccgatt tttcttttga
     2161 gtggctgaca acgaaaagta acatcgaatg tataaaagaa gccccactcg ttttcgaaag
     2221 ggacttcttt atccatgctg cagatagcct ctagatactt gtctggatca ttatgtgtga
     2281 ttaattggct tttaccagtg agccatcgtt tgacttctct cagtttcgaa tgtggaatag
     2341 tgatcccttc tatttccaaa tcaaaaggtt catagtcatc aaacgtctcc gtcaattctc
     2401 cgcttcggcc ctgaatcgtg taagtttcgt atcgcttgtt cggcataata tctggaagct
     2461 ctgcctcaat gatgcaattc atatcaatta ctgcattccg atttttccaa ataaaatttg
     2521 gttcatctgg attcataaat tgtcgactca aataggaaca cctcccaaat cacgaatagc
     2581 ctgtttgttt gctttagcaa atttacgatt catacgatct aactcagatg gattgtttgc
     2641 atcgactttg ccaatgtgta tatgctgttc aatgctacct ccagaaactc ttccactgat
     2701 ccctttgcgt ttttcttcgt ctgataatgg agtaactgtg gttttaccat tcttggcagt
     2761 cagtaactca ggtccagctt caccaacaat cgcttgtcca ttgattagat gaccgccttg
     2821 tgctaaataa ggcagttttg aaatagaaaa tgattttcca cctacaccag gaacccattt
     2881 tggtatcttt atattattca atccaccaat aaatccattg atcaatccaa tcatggcatt
     2941 aataggagct ttccctacag caacaatacc atcgaaaata cctccaaaaa tatcaactag
     3001 accttgccat gcttttgacc aattccctgt aaatactcca gtgataaaat ctagaaaacc
     3061 actgaaaatt cgttttccag cattataaaa attattgaaa tttgctataa caccatcgaa
     3121 aaatccacca aaataaccac ttaaaaaatt aaatatttca acagctacat ctgaaacacc
     3181 tttgaagaaa gaattcactc catcatgaaa ccatttaacg ttattatatg cccaaattag
     3241 tccagtaact aatgcgccaa tagctataac tgctaatgca aaccagcctc ccgacattcc
     3301 aaataaacca gcaagtcctt gccaaactcc tataaaactt ttaacttccc caacgatttt
     3361 tgtaacagat cccataagtg aaccaagaac gattagtact ggtccaactg ctgccgctat
     3421 accagcaatt gttatgatcc aattctttgt atcttgatct agagaccccc accatttaga
     3481 gaattcctgt aataaggaag ttgctttttc aaaaaaaggc aaaaggctta cttggactgc
     3541 ttcacctacg tcagccattg ctaattttgc attgttcata gcttgatcgg cttgatcaat
     3601 tgggtcgaga gtagcatcga atgtgtctcc tacagctccc ccactttctc cagcagtttt
     3661 tgctaaatct tccaggttca aagtacctct acgaattgca tcggccattc taggaccgcc
     3721 tttagtcccg aatacttctg ctgctgcatt gatggcttct gtttcagaac ctgcattttt
     3781 taccttgtct tgtagttctt ctagtccttg acttaaagat tttccgtctt tcgcataagc
     3841 cactgttgct ttagataaac tacttaaagc tgcgcttgaa tcaacaccag attgctcaaa
     3901 ttgtccaagt aaagtgaccc cttcaccaaa gctcaaccct aattgtttaa tttgtggtgc
     3961 gccatcaatt gctttttgca tcaagtcgtc tacagattga ccagtattct gcgatgtttt
     4021 cgttgttaca tcaaggacag aattcaaatc atcatattct agtccataag catcaatagc
     4081 ttgtcgagca gatattgctg attgcgaaac atctgtatca ttgatttcag cgtactgtag
     4141 gagataattg gttgaatctt ccagtttttt atccatgaat ccaaattgtg tatttacctc
     4201 accaatagct tccccaaccg tttgtaaagg taaatgagta tttgaaccaa cgtttttgaa
     4261 agactgtgaa agtctatcag cttgatcacc tgttgctccg gtttttgtaa tgatggtatc
     4321 aagtgcctcg tcaacttcac caaatgctgc aagtcctgct gctcctgccg ctacaatagg
     4381 cgcggttact ccaacagaca ttttttcacc aacgcctttt actttctcac ctgtttcttc
     4441 gattttctgg agtttttttg ctgtatcaac agaaacgtca ccttgttctt taagtgcatc
     4501 gttcgtctgt tctaatgctg tacgaagttt attttctccg gtttcagaat tgagtaattg
     4561 tttataaagt ttttctgatt cagcagaata ttcaccggtt tctttgacag atttttcata
     4621 ttcctctctc agcatttgag atcgttgttc agccaatcct aattgttttt ctaatttttg
     4681 tttagccgct cgcaattttt ctgtttgagt tgcatccttg tccatcgcag acacttggtt
     4741 tttgtactcg gcagcggcta agttcatttc tttgttgatt tctttgattg ttttcgaata
     4801 actgacttcg ccattcgttt taaaattaag aacgacatca gattctttac cagccattta
     4861 tcttagcgct cctttctacc accaaggcga tttatccata gttacagatt gaggtggttc
     4921 aaaatcggtg ttttgttgta accactgtaa ataagatttg agccacaagt taggtgtaga
     4981 ctttaaaaag aaactctcac tccaattcaa tagagtgaga gcaacgtaaa tataaaaact
     5041 ccaaggagtt cctatctctt ccgattcttt ttgtttttct ttctttttgc ttgcgctttt
     5101 tgaaagtctt gtggcttctt ggattttttt aggtcttcca cctgaaatgt ctggctagca
     5161 aaaatttcca tacaggtact ataagcagac aacacctctc cactcattcc caaaaatttg
     5221 aaaatagttt ctggatcttc ctctaaacca ccagtacgca gcatggcata aatcaaggca
     5281 cgcatgattt ttagatcact aggcgataag ttagcagaag aaatttgtcc ttctttttta
     5341 gatagcatgg cgttcatatc agattcaaat tgcgaataat ctccaccata cacatctgct
     5401 ataaattcca tcgtttgcat agtaaatgaa atagggaatt ctgcgccttg aataattaca
     5461 acagcagaat tttttaagtc ttcaacgtga attccataat cagataaccg agccattaac
     5521 ccgcacctcc aactttagcc aatgttttcc actgttcttc atcgtataca ggttgagcaa
     5581 tgaatttatt aaataattct agagaagctc cgtctctatt tgaatcaaaa cttgcataca
     5641 tcacattatt gtatttcagt ccgatggaaa ccagatttgc agttacatcg tcaatttttg
     5701 tttcttcctc tgcagtggca tactcttcgt caatcacatt ggatagttgt gtattaggat
     5761 accaaactgc ttttttaccg ccattttcaa tatttccaat aaatccaaag gcgaagtacg
     5821 gaaattctcg tgcggtattt ttgctaaacg ttactccgct tttagcaact aatcctttga
     5881 tttcatccat cacttctatc ggaataccca cgtgatccaa cgcaagttca tgttttgttt
     5941 ctcgcgacac acgacgaaac attttgctgg atgcccattt ttctaatgct gttccattcc
     6001 ctttgacacc gatttttgtg gcaattggca atcgtaaaac ttcactataa gtcggtgcag
     6061 ttccaacaga atctggcgtt gccatcatcg caattaaaat gtcgtccaat ccttcaaaat
     6121 aaaaagtatc ttgttttccc aattaaaatc acccttccca taaatcaagt atttttctag
     6181 tcataatttc ttcaattttt tctttgtttt gttcatacgt accacttgca aaatgttggg
     6241 cacgttgatt tgttgtgcca ttttcggcaa agcgccagta aaaggccgtc tcttcaaatt
     6301 gaactttcac ttgatcgttt tcaactacta cttttatttg atcgcgcata tgtttctttt
     6361 ttaagagcga cttaggaata ttaggaagca actgctttag ataaaagtta gctgcttctt
     6421 ctaaagattc caacgataat tttttcggat caacttttga aaggtttcct aaataatccg
     6481 atatttcaga aaaaccattc ttattacttg gcattttcca cacacctcac atatgtgtaa
     6541 tagttggtta cggtatcgtc gttctcatca ccttgtatac ctgtaaaatc tgaatatgga
     6601 atgccagcat tttgcagcgc ttgttcaata acaactaaat cctgttctgt tccaagtgta
     6661 aaaaaagaga cttggtaata aggtaatttt ttatagactt taccggatgc catttttttg
     6721 ctgttactca catttgagta cacaatgtat ggatacatcg tccctaatct ggctttgtct
     6781 ctgaacactg ctaactttgt tgatttcagc gctgttttca attcatcaaa gctaatcgac
     6841 ataagctaaa ctcaactcca tttctcttgc atcgggattc gtataaatgc gagtaatgtt
     6901 atacgttaca gaatcaattt tgagcgcact taatttctct gtgatggatt tatcccatct
     6961 gactttaatt cgtctgacaa cgtctgtctt ggcttgctgt gataaatatt tttcttgaga
     7021 agtcacaccg agttcttcat agaatattag acgcttaaat tcgtaaattg tagttggacg
     7081 atcgtttccg tctgttcctg ttttgatgtc tagcaattcg gctttccatc tgagattatt
     7141 ggtctgtctc ttcggcattt tgaatcgctc cttgcacgat aaatggcgtc atggcattca
     7201 tagctttgtc gagttcatcc tctgaaactc tatattcata ggcaatgccg gcaaccatca
     7261 aaataagata ttcttgttgg ccaccagttg ctgttttgac ataatctttt gccatattta
     7321 aataaaaaga gagcaaagaa tcatccatgc cctcttcaaa atgaatatgt gatttgaatt
     7381 tttcctctaa agacaattct ttagtttgct cttccatctt aaccaccaac tggttttgta
     7441 atttcgtagc gatatactgc cggttcaaat ggagaataaa ccaattgacc atctagcaag
     7501 ttgtaaattt ggaatccaat ttgattttta ccagagaatt tttcaacaag tttttgaatt
     7561 tccaaggcac caataacttc ttgaatttta aatgcagaaa aatcgccaaa atataaaact
     7621 ggcgtgtctg gttcaccctt tttatctgct gcatctgtcc aatccacagg atagccaact
     7681 aattggtaac caattccacc ttctgcttgt gtgaatggcc gcaacaaagg aaatccatca
     7741 tctgttttca ttttttcaat ggcagtcaaa gcagctcgat taataataaa gcgtcccttt
     7801 ttcatcactt ctgtcactgg tgtattttta aattcgatta atgcatcata taatttttgc
     7861 ccagcacctg aagcagttag atctaaaggt tttttaaatg ctacagcctt tttggctaat
     7921 gcaccaggat tttcatttcc agcgtcatca ccattgaaca tataattgat ttctttacgc
     7981 acataagctt ttttcaattc ttccacaaca atatcttcaa ctggaacacc agacattttt
     8041 aataattttt tagttactgt tgccaaagca tcgaattcgg caggattaag caaaatttca
     8101 tcaaactgaa tagctgtttc agcaatatca gttgaacgct ctttcttgtt tacattcgca
     8161 tctgctttct tcacaagaat tggatatttg acatctcctg atgttcgcac cactgttccg
     8221 tatttacgaa gtaaattttc ttcttgagca taagtaataa cttcagatgc aattacttct
     8281 gggacagtaa ctgaaccgtt gccagcttca atccctaaag ctcgagcttc tgcttcagaa
     8341 atatttccag ctacaaaatt agcaaatgct tttcgtagtt gttgatcttt ttgtttcttg
     8401 gtcatttttg cacgtgcctc caatccgttc ttaattgatc caagtaatcc atctcgttgc
     8461 tgttgagtaa tcattccaga acgattttct gaactatctt cactgtctga atcttcttct
     8521 tgatcgttgt cttcaccaga acggctttcg tctgaatccg tactattcga ttgatcatct
     8581 gtattgtcgt ctgtttcatc agttccagaa tctgcgccta attcgtcttt aattccgttc
     8641 aattcatcaa taacactgtc aatttcttct ttcacggctt ctaaatctgc ttcgcgtaat
     8701 tctcctgatt caactttttc acgtaattca gtcaatcgtt gctcacgacg tgccttcatt
     8761 tttttcaata attctttatc catgtatttt tcctcctacg cttctagcgc ttgattaatt
     8821 tttttgatta atttttttct ttcttcgacg tttttttcta attcatcacg acttcttaat
     8881 gcagcttctg tatcttcgta tgctggcaaa ggcacaatag aaacctcgta taattccact
     8941 tcatggattg ttcgaagcat tggttcagaa ttgtagtccc acgtttcttc agtcggcaca
     9001 aaaccaaaac tacattggtt gatgtctcct cgttccatag atttaactaa gtcccttgct
     9061 accgttgtgt ccggtaaatc aacttcgaat ttcaaaccac gttcatcttc ttcaaggcga
     9121 agggttccgc ttttagtacg tcctagcaca ttggaccaat cgtgattgaa caaacaacga
     9181 acatccgaat tactaattgc ctttgagaat gcaccaggaa caatcacttc gcttaagtca
     9241 tcccataaaa gtgtttgact gttgaataca gcagcgtagc cgctaatggt cctcgtatga
     9301 gtttcttcat cggaacgtgt tgaaagattg gtgatgtcaa tcgtgcgaat ttccttcttc
     9361 ttcatttcca tcacctcctt tcaagtcttg atcattcgtt ggtaaggaat catctgttgc
     9421 atttttctgg ccaatcctag ataagtcatt tgaaatatag atagcttgtg tttctggagt
     9481 attctgttta ggaaaaccaa gcatttctgc cacgttatct ggacttgtaa tcccggtacg
     9541 aacgatgttg tagccaatat ttgtttttgt tgagtaagga acaaaatcca aaatattaat
     9601 tttccattcc actctatagc cagaattagg cataaaaaaa agagcggtgt aatgttcgct
     9661 cttgtttttc aatattggtt tgattgcttt gttgtgcaga tacatcatcg ctttttcaat
     9721 atctgtcttc atcagcgatt gatacgtatc aacattgatt cctagaaatt ttcctaagtc
     9781 ttttttgtaa acacctaaat aattcaaaat tgccgcgtca tcaacaggac tttttaatgt
     9841 ctcgatggaa tatccttttc ccaaaggaat catcttaaca gaatgatcac tctcattttg
     9901 cgttccttcc agttgatcca atatagcttt gacaattttt gtttgggcgc tattatttgg
     9961 attgatgtgg gcgtctagtt taagcatgaa cgcaagtaaa ccgcctttag tatatttgtc
    10021 cgtcaaaact ttttcagcgc tcagaacgcc ttctagagtg tttcttgcaa gatcaattat
    10081 tccagcacct tttaatgaat cagttccgat gttcttaatg tgtcgaatca tttgaccagg
    10141 tattttttga ccattcattt caaattcttc ttgaagtcgt tcattgattt tagttgttac
    10201 accgtacgcc aaatgaagct gatccctatc cgttaatggg aatgtctcac cattgatcaa
    10261 taaagtattt gtttccaatt tggtaaattc gaatcctgtc aaataatcat tgggactctt
    10321 caaaatattt agtaagtgat gatctttgac ttcgttgccg tcgggaccaa taacaattgg
    10381 tgtggccaat gccacctgat tagatatgtc ttgaactaat tcataaacat cagaagattc
    10441 catgatagat gaatccgtca catatctttg accataacgt gtgtagtgtc caaaaatatc
    10501 ctcgatatat ccacgttttt ccataaaaga atagactgca ttcgataacc gatcacgtaa
    10561 tttcaatttt tttcaccgcc tttctattta tctatagatg gaacttaggt aatcatctaa
    10621 ctcatctgaa tcaatatctg tcatttgatt catcgtttcc ttatgaccac acaaaaacgc
    10681 cacgaaccca tcaatctttt tctttgattg acgtttactt ggcgcttttt gtccattgat
    10741 gttagtaatt gctacaacgt tcaaagtgca ataaaggaat aatggattat caaattgaat
    10801 tcgtttctca taaaacaacc gttcgacatc gtcaaaagga gcattcaata ctttaggata
    10861 ttgagcaact tcaacgcatt ccaatcctaa gttttctaat ttctcaacaa gtttgtcact
    10921 catcgctgga tcataattca cttgctgaat atcatataaa ttcatgcagt cctctatgta
    10981 ctgcaaaatc tgatcttgat caatcatttt tccgtcgcaa aattcaacga aaccttgttc
    11041 agccaaatca ctgtaaggaa cattatcttc tttttctcga aactctaaat cttcattcgg
    11101 aataaaataa agttgcttta ctttaagaac cgctttacca tcttcatccc atgttgggaa
    11161 gtttaaagat acacaggtca aatctcgtgt acgtgataaa tctaaaccga tataacatgg
    11221 ttcgccactt aaatcaccaa gttcttgtgt ggtaactaaa catggctcta cttgatcctg
    11281 ttcaaagaaa ttatccgcgc cgttcacaaa tacatccaag tgtttcgtta gaaattcagc
    11341 tttggaatga gctgaccgtt gcgctgtttt aaatgccgat tctaaagcag ataaatcgac
    11401 tgatattccc cagttaggat tgcacatttc ccagactttt ctatccgtcc aatcataatt
    11461 tttatttggc tcatagatta gaacaaagtt tgaatcatta tcatcacgtt tcaacacttc
    11521 ttttgcttcg cgatatacac gcattccaac agacgacgaa cctttcccag cagttgagat
    11581 attaaacatc aatggctgtg gtaaagatat ctgagcagac ttaaaattgt catactgttc
    11641 cattttctcc tgtttatgca attcatcgtt caatacaaaa tatggattgg atccctcgat
    11701 attatcaata ttcttggttt gaacaataaa tttatttgag taagccatat cttcatgaat
    11761 atagtcataa gtaatactag aaacagttcc ctttggcccc ctaaatattt tagttccttc
    11821 aagtaggatt ggattgttta agattgtaac tgcaaaaggt ttggcagcat attgcgcttg
    11881 agcaaaatca gatgcgcaag catagcaatc aactgataac gccccttctc cgtacattgc
    11941 atatcctaat gaaccgacag caatcaatgt tttaccattc ttttttggaa tctggacata
    12001 agcctcacga gtaacgcgta caatttggcc tttttcattt tctttaaccc aaccataaat
    12061 ccaagaataa ataaattttt cccagggttc taaaagaaac ggttttccca ccatttctcc
    12121 ttttgtgtga acaataaatg attctaccca atccatcatt tcattcgcgc gatctacatc
    12181 aaaccaaata tcttttcttt ttttccactg ataccagcga tctattgcta aacgaacggt
    12241 cttaggatac ttcttaggtt tctttcttac ttcttttgca aataaatcag cataatttac
    12301 accaggttca atcatttttc agcacctgcc ttcttacgcc atttatttct gtgcgctgct
    12361 agttcatcta ctggctttac ttctgggcgt gtaatttctt catcttttct tgcggtcgat
    12421 cctccagcaa ttagtctacc agtttttgct ttgtttgtta atcctaataa atctagagct
    12481 ttcatcttct tatctgccca agtttctact tgttgtgcca atggatgttt tgattgatta
    12541 gtagctcctg atttattcgt gaatttttgc gtctccggaa atcctttttc cttccacaat
    12601 aaatatttgt gttggtaaat ttcaaaaata tccaagtatg attcgattaa tggatcaaga
    12661 gtgatggtgt acaaatcaga caaattcatt atttctaaga tacgagcttt ttcagcactt
    12721 actttttcat caataatcgc tttacgttgc gctttagtcg tcatttttgt atacaccccc
    12781 ctttgttttt tgaaaaattt gacctaacga tgcgcgtgac tccccgctac cctatctccc
    12841 cacgcgaaaa aattttgaaa atgaataggg gggcttcaat tgaaatacga aggaaaaact
    12901 tttttgtctt ccgtttcgtt ttcaacaatt ggatgacatt ttgaacataa tagcatgaga
    12961 ttgtttggat caagcttaag cagttcgttg tctttgattg gtacaatgtg atggacgtgt
    13021 gctctctttc caaatatgaa ctgaccacac cgctgacaat gaccaccttc tctttcataa
    13081 ataaattgac gcatatcttt ccatgcttgc gttcgataga atggtttgtt ctcatgatga
    13141 taaacagact ttgcttgctg cttcttcttg cgtgatctgc ttgatctctt gtgttcagta
    13201 cagtagatac cctttgctat tttgttcgtg catccgtcaa actgacagta tttcattcta
    13261 cttcacgaat aagatttatg atatcgcctt ttgcacggat agcaccagga atatcaatac
    13321 catgtttctt agcatatgca cgcaattctt ttgcagtcat attgtccagt tcatccgtat
    13381 cttcagtgga ctgatcatta gtagcttcgt ctccatcaaa attagcagca gtgtttccat
    13441 cgctatcaag aatttcactg ctatgagcta tgagttcgcc attgacagca acaaatgatt
    13501 tgccatcact caaaattcct ttttcttctt ttacttcaaa atttggttct tgatctttcg
    13561 gaacaactac tgtccttttc ttttctgaat cccaatactc tgttccacta atggatgttc
    13621 taattttgat cattgccatt ttaattctct cctttgtaat ttgtacggat tacttttgcg
    13681 cccattcgct cataccattc aatcgtttcc tgcagattgg gtagagtgtg tgacaataaa
    13741 gaaataacta acgtattcat tccagttctg taatcatcat tctccacttg cacatgtgca
    13801 tgattaccat tccaattttc tctgtacgga ctattctctg cttcaattgc tttcttataa
    13861 actttagcca aacttgattc tacttcaata ctaagtaccg cttcatgaaa atcattcatt
    13921 tgtttactct cctatcagat attttgtggt atgatttata aaaaagatgc gaggtgtttt
    13981 aatgcttcaa gaagttaata ttaagtatcg tgatcgtcaa aatacagaga caatttattt
    14041 gccaaatagt tgtccgcatt gcggaaatat aatgactccg tatgtgtatt ttggggtttc
    14101 atcatatagt gcgttcgata atgatagaat ctttggcgta ttagtacagt gtataaatga
    14161 tgagtgcaac aaattttata gtttacaatt tataaatacg tttaatgaag atgaaggata
    14221 ttctgaaaat attttaatta aagattatta ccgtccgccg attaaaatag atttaccaga
    14281 aaatattgaa aaagtttctc ctacatttgt tgatatttat actcaagcca cacaagctaa
    14341 atctgagcaa ttggaacaaa ttgctggagt tggttataga aaagctctag aatttctaat
    14401 taaagattat gcaatatcta aaaattcaga ggatgaagat aaaataaaaa aaatgttatt
    14461 aggtaaaaca attgaaaatt atttgaatga ttttcctaaa ctccaaagat tagccaaagc
    14521 tgctacttgg attggtaatg atgaaacgca ctatgttcgt agacatacta ataaagatgt
    14581 tcatgatatg gatgcattta ttaaatcagc agcgcaattt atcgcggctg attatgatgc
    14641 tgatttagct gatcaattta taaatggaaa taactttatg ccaactacta attaaaagaa
    14701 taaaagagcc acagatacca attaataaaa cggttgggat atcttggctc tttcatattt
    14761 ttttgacact aatagaatat catgctgcta acaatgttta catagctaca atgaccgaac
    14821 atttagtgaa cagtgtaatt ttagaataaa gagagttgtt ctttgtactt ctcgctattg
    14881 taaaaatcaa tttcgttttc ttttttcaat cggtcagctt gtctgtgttc atactcatct
    14941 aaaaagttca atgtctttct aatttctgca tgtcgctgtc tgacatacga tgagctatac
    15001 ccaacagatt cagcaatatc ttctaagctc atttgatcaa catatttcat cttaacaatt
    15061 tgattatcga taccagaaaa gctatctatc agcaataaca tttcttcttt ttgctcgatc
    15121 aacaattcca attcattttc tatcttttgg atattctctt ctaaagaaga agttctcgaa
    15181 ttcttttcga tgcggacgtt tgctaaatca ccacagaccc atcgattcaa ttcaagctta
    15241 cttttattaa gattccactt taagtaaaga atctgctcat ctagttcttg gtaatctttt
    15301 aaccaatgaa atctcacaaa cgccacccct tatggtagaa tagtattgcg ctgctatctc
    15361 gaaaggggta gcttttttat tttttcttag ccgttccatt ctggattgcc ttttgttcca
    15421 aacgtcgttt tttcttctta atctttgatt ttgtttttcc catacaattc tcctttccga
    15481 aaacacattt tgttcgattt ttaatactaa ataaaagttg gccagaattt ttcgttcgtc
    15541 tttactcatc tttttttgaa taagattact agcataaaaa tagttagtac cacgaaaatg
    15601 aacaggtaaa tcaattacgt tcaccttctt catgccagtt atcagccact agaatctttg
    15661 cgttagtgcc gccaccgttt tcttttttgt atttttcaaa tgctttataa gcagctgttt
    15721 ttgatttata gatttttccg gaaccgactg ggaacatcca tgtgccctct gtgatagcta
    15781 agatgtatac tttcatttaa ttcacccctt taatccccaa cactacatat ccggacttct
    15841 gcatgtaatc agtaatataa gtgatctctg ctttgtatga gtcaccagtg taactaaatg
    15901 ttggtctcgt tagtccaggt aattcatgtt taatgtattt tttatattca cgtagtatta
    15961 actgatcacc aactttgaag tcacgatcat tcttgcggat ctcgaattgt ttgcgtccgc
    16021 tcgtcactgc ttcaaaatat tctggtagta tttttagttc gtggatcatt ctgtttcctc
    16081 cttcaaaatt cgctcatcat caaaaagtga atctccacaa aatccttctc ccaaatattt
    16141 atcgttcatg tataagtcaa ccaaatattt gtgatctgct tctcgatatt cattaatatt
    16201 aacgatcttt attttgtaaa cattaccttc gtcgctcact atcttgaatt gttctctgac
    16261 tttcaccacc gttcctcctc tttttgaaaa aattttagtg tttttgtaag accccctaaa
    16321 tcagcttttt tatttgatat aatacttgta tcgattaatc aaatcgaaat taaatttaaa
    16381 aaggtgattt atcatgaata tcaacaattt agtaaaactc gttgaacaac gttttaaaga
    16441 atcaggatta gaacaattaa aaaaagataa ttctccagaa ttattttcta attttaccga
    16501 agacgacatt cctactctta aacaattaaa atattattct gattattgga cgcaaactcg
    16561 tgagaatgaa ttaaatttag aaaaactcat tattaaaaag aaatagcaac tccagcccac
    16621 acggctggtt ttttagtttc aacttccctc cttacgggtc aaaatcatcg gttccggcat
    16681 aatctgatca tcctttagca acaaatcagg atattttttc cttagcttct tattgttcgt
    16741 gtaagttttc aatagccatt ggttggcttc gcttttgctt tctccagaaa aataaatttt
    16801 ccctgtttgg atgcctgcaa gttgaatcat cattttcacc tcaaatcaaa ttttttctgc
    16861 tagatggagc aaaagcaatc cgacatcaag aatcttttga cccattattt cagctacttt
    16921 gctcggctta actccttgag aaaagagcaa gcgtgcttgc tctatctctt cgtgagtcca
    16981 aataaatttg gcttcctcaa ggataattac tggatcagtt gctctcattg ttcctctgga
    17041 aaaatgatgt cattcttatg cttggaccag gtatctgcat atggaacaaa gtattgtcta
    17101 gctaattcga tttgatcaat taaagcagtt tctgataatt catggtctgc agcaatttca
    17161 ttcatactat cgccttcatc taaacagata agaccgttga cgttatcgat ttttacattt
    17221 gtgactgctt gactttctcc acgctcataa tattctacag ttgatactga tacatcagct
    17281 tcggagttta attgcagaat ctgttactgc cgggataatt taatattctt ttcataattc
    17341 accttttttc ctactgacat cgaatatttt tcatgttatg attagccgtg aaaggagagc
    17401 tatattatgt gtttacacat gatgtcctta gctcattcgg ttctttggaa gcttttttgg
    17461 aaataactct caggttcttg acgcaaatct catttattct ttgaaacaaa aaacttccat
    17521 tttccgagag ctttggatca cttcaaaaga gatttgcatt tatatttctg tgaccttgag
    17581 tcgcgtaaag aaccttacag caatggcggc aattccactt tcaggaaacc gccttttttc
    17641 attgatattg gtggttagcg gactacccat tttctttcgg cagtgccaaa ccacaaatga
    17701 tctgtgttga tgtttaggta gttgtatacc agataagcta aatccaaagt gcctgcatct
    17761 tcaagacgac aatattccaa aaattctccc atagctggac ataaaaatcc tggagctatc
    17821 atatcccaac agttaatagt gcaaagaaat tcgtttagct tctgaacgtt ttctttggtt
    17881 tcacgaatta caacatactc caattcttct ccgctggcag agcattccaa aacttcaaat
    17941 tgattaagaa atggtagaac atatgcttca aacatcaaca ttccatcttt ctgtttttgc
    18001 acttgttttg catcaatcat gtctatcctc ctattcagtt atttcttcca ttaatggaat
    18061 tatttcaggt cactggactt aacgaaaaca ccattgacca tctttccagt ccgacctttg
    18121 atttcgtcat aagcaaaatc caagcactcg tataaatcca tatcattttg cattgccaag
    18181 atgatcaagg ttactactac atcgccaatt ccgtctctta aaccatgttc atcttttcta
    18241 gctagagaag cggcaacttc cccgatttct tcgaccgttt ttaacatttg cttgctgaaa
    18301 tccgcttgat ccaatccctt atcttttgcc cactgctcta ctttcgcgat tagttcgtcc
    18361 attatttctc ctccacatac ctaaactgtc ttccttttga atcaatccat aagctcctag
    18421 ctctatccca aataatattt ttgctcagac cagtaatttc agataactgt ttagcggtac
    18481 ccgttactag aattcgatca ccatgccaga ttgcaatctt tctcggcgtt ctccgtttgg
    18541 gcttttcagt ccacattgat ttaccgagct tttggacttc tgcaactatt tttttatctt
    18601 cctgccaatc ttctgaataa gtcaattcga taattcgctg cattgccgct ttcttatcca
    18661 tcccgacatt ctcctttcag taatttgagt acttgatcaa gtgcgctctc acgtccgcca
    18721 tgaaatgtgt tgagccactt gtcttcgtac gacacacttt gtcttaaagc ttcttgatgc
    18781 attaattcga tctgtgctgt aaatgtcttt aggtccatct tgtttacacc tgctcaagtt
    18841 cattaagatg tttttgcaag cctttaacac aatcaatgaa tagtaatttg gtatatgcta
    18901 aatttcttaa ttgcgttcca tcgatataga gtgcgaaata gtatctgagt ttactccaac
    18961 tcgaacgatc attcttaatt ccttcaattc cagcttcttc gagttgatca tatacgtctc
    19021 tcaggacgtc taattcctcg ccctttttat aattcgctat ttcattaatt agttctagat
    19081 aatcgatctt catttttcca cctcttagaa tggtgctttt gattgtctat tagctcgttc
    19141 tagcgctttt ttcttgagat aggcttcttg gtcgattgcc cattcaggaa gcttctctcg
    19201 tcttcctgtg cgcttgtatc cactgcttgc gttcttaggt tcactttttt ctttccttgc
    19261 ccaacttcga atagttgcca aatagttttt ataagtctta ccagatgatt cacaatactc
    19321 tgacagtcgt tcgattcgct cttggtaatc attagggaat tctgttttga gtttctccat
    19381 ctgctcatct gacaaaagaa catttttata ctctccgtat ttatgacgga cgggcttagc
    19441 cttcgatttt ttcgaaggcg gtaactctct tatatattct tttgtattat taaatgtatt
    19501 attaatagat gtattattat ctttgacttt ttcgtcaata ggggtattgc gtttttcgtc
    19561 aatagggtat tgattaattc gtaggtacct attgattatt tgattggtac cctctttgta
    19621 aatgatttcc cgattcaagt atccaaactt aatcaaatca cttacccatc gcgatatggt
    19681 ctctttattc acaccatata aatctgcaaa gtactcattg cctgcccaac aaaagcctct
    19741 ttcattacac aaggccgtta tctctccgta taacaactta gtatttggtt taagtctttt
    19801 gtcgtacctt acgttggctg gtataatcgc ataataactt cgatgttctg tcatttttcc
    19861 cctccaatat ttaacttttt gattgttttc tggtttaatt tgatcccttt gatttgatat
    19921 ttatttttga aattaatcac acctattttg tgcttctctg tgtgatggat tctgcagagt
    19981 gctgcaaatg tgtactctgc atgatcaact tctttgcgct ttcgtcttcc tagcgctttg
    20041 tcaaagtgat cgatgtcagc tcctgttttg ccacagatgc agcagactct ttttgtgatg
    20101 catttgtaga agtaatattc ttgattcgca ggtaaaatct catagccttc tttgaaagga
    20161 atatgatgtt caaagatgaa atctaagatg atatttgcta agacattggc atcactcaca
    20221 gttgtattcg attcgtcttt caggcttatt ttgcgccctg tgacaccttc aaaacgaaag
    20281 tagaagaatt ccttccagaa gtccgttggc atgcctgtat cgataaaaat atcgcctatc
    20341 agcgcataga tgaagtttcg ttgctgtacg gtgaaacgtc taggatcaat aaaacgaatt
    20401 tcaatgactc gatcaccatc gtagccgtca tacatcgtct ttagtcgatc aatgttcact
    20461 tcctcattga tggttgcgct tatgtctttt cctttgaact ttttcagaac cgctgaatat
    20521 gaatcgatta atggtttaaa cactcatatc acttcttatc taattctttt ctcttagctg
    20581 ctattgctcg ctccatcaag gcacattgct catagcttaa ctgttcaata gtttcaacgt
    20641 tatcagctaa gagccctaat ttatctgtct gctcattaac atattcgatt aaggttttgg
    20701 tcatatcttt acccatctgc tcattgaaag cttctagaat cgtctctagc atgtttaatt
    20761 tctttgtatc gattctaggt ggtgttggaa tatcttcccc ttgaaataca tataatccca
    20821 gtccgtgtag agccaatgct ttcacaaagc atcgcttcaa tgagttattg atttgcattg
    20881 catttggttt aacaactggt tggtttcgat aatctaaaac aggaaataat tcggtttccg
    20941 tgtgtccttt aaccgttact gagacagata cataagtccc agtttcatcc ataagaaaag
    21001 gtttatattc ctcaacaaga aagtcttgat gagttccaga aacaaccctg tagtgtttgt
    21061 actcattaat agttaccgtt gcctgtggat cattcttttt cataatctcc cacgcgtgag
    21121 cccaagataa ataatcaaaa tttccttttt tcttgagaat tttatttaac ttacgactaa
    21181 aaagtttttc aaagttcgtt gtccctttga tttcactcat caaattctgc ctccatttca
    21241 gcaatgtatt tcttacctgg tccgtaataa gagatatcaa tcaagttatc tctgtcgtac
    21301 tcttctagcg catcaatcaa gccatcttcg atgacgtaaa tatattcagg tttattcgaa
    21361 tgcttcgata gatggataag ataaacatga tcccaaatag ttacatggtt gcctaaatca
    21421 tcttgatccc aagctagttc ttcattcgtc aaaagatttc gtctgatttt tcgaccactt
    21481 gtttcctcaa ttttcggctt gccccaatca ggatcaatca aatattgatc tagagtggaa
    21541 agttcttttt ccatatgcta aaatctcctt atgatgtgtt ttctttgtga ctctttgctt
    21601 gccggcggag tcactttttt atttgttgcc atgctttttg cttgtcaata tgttgttggc
    21661 ttaggatgat tggtttatag tatttccacc agcaattagc aattgccgtc cctattctta
    21721 gcgcttcagc tctattcatt gtcatcacca aaaagtctct gttgtctgtt cagttgatca
    21781 atttccatac ggatcgcagt ttctggcaac cacatttcaa taaatgaaac agcatcatcg
    21841 aatctcttac gaggtaactc gccatatctt gggattgaaa aggtacgttt aaattcagac
    21901 caaaattttg agaatacttt tttgctgatt tcttcataag ctcggctttc ttttccccct
    21961 agaactccca taactttcat atttcctttt tgcttaattt caaactcttg ttgtccgcta
    22021 attcgcatag tatctttaag catggaaaca tcttttttaa catctttcat ttcttctaat
    22081 tgatagatca tcatatcttc aattgtttga ggaacagtat tcttgcgaat aacatcttcc
    22141 atttcgttga atgcttcaat gtatttttgt ttgaagtaga tagctttctt tcctgtaaac
    22201 cccatagcca gcaagaaaaa tccatctcta ctaatgaaga aaactcgtcg atttctgcca
    22261 tatgaatctg gttcattacc ttctacaaac atctgtccaa aattggacac atcttttttt
    22321 agtgcatcaa tatctcttaa aacatgttga tgttttttct cgaagctttc tgccacttgt
    22381 aagctcgtag tcacagcttc tttatttttc aaaattacta attcttgcat tatttcttct
    22441 ctcctttttg gtataattta gttaaaaact ggtggtgttt attttgaatt tttttcactt
    22501 aacaattttt caatggatgt caataataag ttttatattg agtacgtttt taacagggat
    22561 aaaaataaaa ggaatgcgcc ctcaactaga agttgaactt aatgcttctt acttcgctgc
    22621 agatatgatt tatacaaaag taatcatttc taactattca acagaaccag ctatgttagt
    22681 taatttagaa ctttcatccg ctaacttaaa ccgcagatgg tcagctactc catttaagaa
    22741 attaattgct aaaggaggat cagtcgatga taatagaata tactctgaag ctgtaccgct
    22801 aaatattcct cctaaaagtg ctatatcttg ttaccttgca tttgaagtag ggaaaattaa
    22861 ttttaataaa ctccttaatc accaaacaaa aatgatattt actctaaata gaacccaaat
    22921 ccacaaaatt gttgatatca aaaatacaaa cttcccaata gagaaattag tga
//
LOCUS       DAZKLM010000043        22721 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3764
            SAMEA115706913-rid22582563.denovo.043, whole genome shotgun
            sequence.
ACCESSION   DAZKLM010000043 DAZKLM010000000
VERSION     DAZKLM010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706913
            Sequence Read Archive: ERR13196647
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22721)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22721)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287823.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287823.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,004
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,849
            CDSs (with protein)               :: 2,849
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 41 of 96
            Pseudo Genes (incomplete)         :: 52 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 15 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22721
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706913-rid22582563.denovo.043"
                     /isolate="VRE3764"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            71..217
                     /locus_tag="ACG842_001513"
     CDS             71..217
                     /locus_tag="ACG842_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EntF family bacteriocin induction factor"
                     /protein_id="HFQ0248061.1"
                     /translation="MEEKNRLNAKQCSDQELKKIKGGAGTKPQGKPASNLVECVFSLF
                     KKCN"
     gene            293..1540
                     /locus_tag="ACG842_001514"
     CDS             293..1540
                     /locus_tag="ACG842_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HFQ0248062.1"
                     /translation="MFVGNFFFFLLFYMTNTDLNKRKYLFVCLLIPLTFFVELFTDVS
                     AAIPIIGSYFILKEKKKTNIVLLNKLLLCMLIDDFRSIITSMVMTYMFSFEGIKGYGY
                     VVFQIMLDSLFLLMFIWLYRKLAINRIVEQSSSKLTAIFMIYLLTIGLFVSYVAHQYQ
                     VFDHFVFGVLIFLIIQMFVVLFLFIRITIRQREQYEQQLKRKELVYLKKYTDSLEKDQ
                     EKFARFRHDYKNLLLSLKEIANQNHDSILEKEIKELEIYSNSYLTNKFEYRYLKNIKN
                     EYLKSLLIAKLYQANNNKICCKFECPSTIIEVPVPIFDCIRVLGIILDNAIEASIESQ
                     DRELSLAIYRDDKQIEISISNSCREILLSIDTLMKKGITTKKGHQGLGLSSIEEINKK
                     NPNMFVNYQNDPYQFTTQVILLF"
     gene            1555..2307
                     /locus_tag="ACG842_001515"
     CDS             1555..2307
                     /locus_tag="ACG842_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFQ0248063.1"
                     /translation="MSYPIILCEDQIIQLNQLERIIDNFILFHDKVFKIVLKTQSPLE
                     VKKYLKQFRPKQGIYFLDIDLNHEVNGIELAEVIRKYDVQAKIIFTTTHDEMLPVTIK
                     RRVETLGFVTKDQTLDEYRNEIVELLLLAQERIDATKQVTNQAFVFSIGSQTFTFDIN
                     DIYFVEASKLPHRLSLCTKDGQYEFYGRISELEKKYPMLTRISRACLVNIFNVKEIDF
                     KKRSLYFDSELARNFTLGKAQKIKEKLKESTV"
     gene            complement(3010..3225)
                     /locus_tag="ACG842_001516"
     CDS             complement(3010..3225)
                     /locus_tag="ACG842_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042742 - defense response to bacterium
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Blp family class II bacteriocin"
                     /protein_id="HFQ0248064.1"
                     /translation="MHIKNTKTTFILSSEELKNIQGGSAVGVLGTTFSGATAGVKLCS
                     AGGPYAIAACGVGGALLGAGFSMWTGA"
     gene            complement(3244..3480)
                     /locus_tag="ACG842_001517"
     CDS             complement(3244..3480)
                     /locus_tag="ACG842_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin"
                     /protein_id="HFQ0248065.1"
                     /translation="MFKHAKCKGTKYCGNAQTVGGGNNAWGKLGQVVGGLTTGAVGGA
                     GLGTAICGPACGVVGGLYGAVAGGAAAGWDARKK"
     gene            3738..5891
                     /locus_tag="ACG842_001518"
     CDS             3738..5891
                     /locus_tag="ACG842_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0043214 - ABC-type bacteriocin transporter
                     activity [Evidence IEA];
                     GO_process: GO:0043213 - bacteriocin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide cleavage/export ABC transporter"
                     /protein_id="HFQ0248066.1"
                     /translation="MIKSFPFVQQQDEKDCGVACLSMILKYFKTDIPIHKLRDLSGTD
                     LEGTSAFGLKKTFEKLSFDCMAIQADSEIWKERDLILPLIAHILVDNSYMHYVVIYKT
                     KGNVLWIADPANGKNRKTIEAFQKEWTGILLLPMPRETYIPIKEKVAGLSSFLPIIWK
                     QKGLVFHIVLASIFITFFGIGSSYYFQGILDFFIPNQARSTLNIISIGLIAVYLFRVL
                     FEYSRSYLLIILGQRMSMAIMLQYFKHVLTLPMNFFATRKSGEIISRFLDASKIIDAL
                     ASATLSIFLDIGMVLLVGVTLAIQNSTLFLITLCSLPFYVVAIFAFVKSYEKSNREEM
                     AAGAAVNSSIIESLKGIETIKAYSGEEKVYNRIDHEFVKLMKKSFRTVTLDNIQQGIK
                     HLIQLISSALILWLGSYFVMDNRISLGQLITYNALLVFLTDPLQNIINLQVKMQTAQV
                     ANNRLNEIFAIDSEKSNQSFEYMLSEQLFQQDITVKNVSFSYSMKAPTLNEISCTIPS
                     HSKTAIVGVSGSGKSTLAKLFVRFYEASEGSIHYGNINHLDIPFQILRDRVTYLPQES
                     FFFSGTIIENLIFGLDSPPSFEQIIEVCEIVQLNEFINQQPLRYETLLEEGGNNLSGG
                     QRQRLALARALLKNPDILILDEATSGLDTLLEHAIMNYLLNLKEKTIIFIAHHLPIAK
                     ACDQILVLHEGHLVEKGTHDELRFADGIYQKLWEI"
     gene            5860..7275
                     /locus_tag="ACG842_001519"
     CDS             5860..7275
                     /locus_tag="ACG842_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HFQ0248067.1"
                     /translation="MVSIKNYGKSRRGKQFMQNNNWFEHSSIYTKQHHSFYRWVLYPV
                     ILFLCIIGLFLTFAKKEVVIRTTAQLNPEKIEKLQVPLEAKIMENFLKENQFVHKDDV
                     LVRLDCSLIENEKAQIEQENQRITQQIKMAQLFIESISKGKNLFSTDDSFGYSNQLKS
                     MLSEKESLRYALKQSELNDQKQLEVYEKTKRQLEKQIESSDSKLQEWQQVQVAWSNNQ
                     SLKDFSKEMMANYENWQEQLNNVSDDQKNQVKLTISASINEQIEQLKKEVEQYQSEKA
                     KLVKPTTSENDRISQTEKGKQELEQAITTVKQTVTELNEKQEKNQSVIKSLDDQLSKG
                     ILKAPVSGTVHLNEETKGQAELAKGTVLAEIYPIHEKSKMKFTALLPANESTYIKEGM
                     KVHFKLDQKGGAPITIDGTLDEISENSTATEKGVFYAVKGTLKTTQSFPFRYGLTGEL
                     SLIVGEKTYWQQIVDVFLNKK"
     gene            7308..8342
                     /gene="sppA"
                     /locus_tag="ACG842_001520"
     CDS             7308..8342
                     /gene="sppA"
                     /locus_tag="ACG842_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HFQ0248068.1"
                     /translation="MNKRRWIAVGVACGLLLLSIIVALIPGKDKEEASNTTLTGINKI
                     FYGSNEITEETLEEGASNKKIVKLSVNGVIADTGESNLFSREQYNHQNFLTQLKKIQE
                     DKAVKGVLLEVNSPGGGIYESAEIAKEMAKIKKLDIPIYTAFKNTAASGGYYISAGSD
                     KIFATEETTTGSIGVIISGLNYSGLLEKLGVTDATYKSGALKDMMSPQHKPSEEENKV
                     IQEFVMSAYDRFVNVVAKGRNMDTNAVKELADGRIYDGNQAVENGLVDQIGYSEDALD
                     SLKKEKKLTDATVIEYKNDTTGFASSWLGNKIAEWQGLKATTSDRVISVFEKLGFSDT
                     PKPMYYYGGL"
     gene            8344..8865
                     /locus_tag="ACG842_001521"
     CDS             8344..8865
                     /locus_tag="ACG842_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HFQ0248069.1"
                     /translation="MNKKSGIETLPNYVFAGFWLRFFAFLIDLICISIITHATIGLFY
                     KIMGLESDKSIITFYGIASLAIYLTYFVLLTKLNSGQTIGKMIFGIRVVSFEEKELSW
                     QTIFIRECICRFILKNFVFQIGYLVAAFTPKKQHIGDYFSNTSVVVIRSLEAINYGQK
                     ISNSKTLNLERIK"
     gene            8862..9248
                     /locus_tag="ACG842_001522"
     CDS             8862..9248
                     /locus_tag="ACG842_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HFQ0248070.1"
                     /translation="MNKRKTEFILILISGITGILCSLLALMGGMFLSSIDSTTLNTTE
                     LTSKDIDMLVNLSGNVVLFGGITLVFSIMIFIIGFLVRKNQKTVVYGISLLILSIIPF
                     FLLTLLWVIPGVLGIIAGLMLLIRKS"
     gene            complement(9744..9816)
                     /locus_tag="ACG842_001523"
     tRNA            complement(9744..9816)
                     /locus_tag="ACG842_001523"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9781..9783),aa:Lys,seq:ctt)
     gene            10197..10766
                     /locus_tag="ACG842_001524"
     CDS             10197..10766
                     /locus_tag="ACG842_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HFQ0248071.1"
                     /translation="MEKMRQQLMEWGKELETFRLPHWEELPDLELYMDQVRTLVDRYL
                     SPVIQGEKHPLLTSSMVNNYVKLGLIPAPVKKRYNKEHVAFLLAITTLKQVLTIPEIK
                     EGILFQGKTVGIREAYNLFCDEQEAAVWMVSQLAQGKSHPQKFDQATPVEYIAVRAAT
                     LSFAMKLLAEKTIVLETEYLKEEKTNEKQ"
     gene            10753..11613
                     /locus_tag="ACG842_001525"
     CDS             10753..11613
                     /locus_tag="ACG842_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HFQ0248072.1"
                     /translation="MKNNKIAILVDSGTDVPEELIKKYEMYMIPLKIIYKDQVYTDKI
                     DITPEEIYQRLPEEIPGTSLPDGESITEIFEQIKKDGYEKVLAVTISSGLSGTYNVVR
                     LIAQQQEELEAYVLDTKNIGIGAGFSAIQAAKWLEEGVEWDQLISNLNELVERTKVFF
                     NVATLEYLQKGGRIGLVASIVGTALKLNPIISCNEEGIYYTVGKARGRKKSLDKTVAY
                     VKEKIGNAKVFNLAVAHGDAKEEAIEMEARLKREFPQANQIYFGQISPALVVHTGPGL
                     LGVGVQVLEG"
     gene            11803..12507
                     /gene="yycF"
                     /locus_tag="ACG842_001526"
     CDS             11803..12507
                     /gene="yycF"
                     /locus_tag="ACG842_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator YycF"
                     /protein_id="HFQ0248073.1"
                     /translation="MKKVLVVDDEKPISDIVKFNLAKEGYDVYTAYDGEEALEKVAEV
                     EPDLILLDLMLPKMDGLEVAREVRKTYDMPIIMVTAKDSEIDKVLGLELGADDYVTKP
                     FSNRELVARVKANLRRGATAAKEPEEAAPAELTIGDLTIHPEAYMVTKRGETIELTHR
                     EFELLFYLAKHLGQVMTREHLLQTVWGYDYFGDVRTVDVTVRRLREKIEDNPSHPNYL
                     VTRRGVGYYLRNPEQE"
     gene            12512..14347
                     /gene="walK"
                     /locus_tag="ACG842_001527"
     CDS             12512..14347
                     /gene="walK"
                     /locus_tag="ACG842_001527"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     WalK"
                     /protein_id="HFQ0248074.1"
                     /translation="MKRKVRFFRSVNFKIAITFILILLISIEIIGAYFIRGLERSTIN
                     TFIKDMNQTVESLATTISPELNRKDNADDEEVNANIKRFIENSATSDIIEIRVVDEKG
                     IIRGTTDVNEQSAVGKKNDYVDINDFTTKRYVALDNDDKRVNINVQPILSPTGDAVIG
                     ALYVKSNIEQKYSEINNTAVIFFTASLIAAFISMIVSVLVARSITQPIGEMREQAIRI
                     ARGDYSRKVKVHGQDELGQLADTFNQLAERIEEAQDTMEAERNRLDSVLSHMTDGVIA
                     TDRRGKVITINDMAVSLLDIKNEEAIGQSILTLLDIEEEYTLRKLLEDPNEMVLDRST
                     STLESDQMILRVDFSMIRRESGFISGLVAVLHDVTEQEKTERERREFVSNVSHELRTP
                     LTSMRSYIEALSEGAWKDEEVAPNFLKVTLDETDRMIRMINDLLNLSRMDTGNTQLQL
                     EYVNFNELVNFVLDRFDMMVGNQEKNYKIRREFTQRDLWVEVDTDKIIQVVDNIMNNA
                     IKYSPDGGTITCRLLETHNNVILSITDQGLGIPKKDLNRVFERFYRVDKARARAQGGT
                     GLGLAISREVIKAHRGAIWAESKEGKGSTFYISLPYEPYEEEWWE"
     gene            14344..15660
                     /gene="yycH"
                     /locus_tag="ACG842_001528"
     CDS             14344..15660
                     /gene="yycH"
                     /locus_tag="ACG842_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system activity regulator YycH"
                     /protein_id="HFQ0248075.1"
                     /translation="MRISEKVVRIGLILMVALSIYFSYAIWLSPAGKSSINVDETNTQ
                     MIESQSNRKTSEVFLPLHVTWHHSGMIQETNSENLVYRLQLIIDKARFGKLSEVVSGD
                     KSQFEQVKNLNSGFELAYNAPFLLREYKEAFDLDLDVSNLAKESENMYFTRIQFDQGN
                     KKIRFLNYSNHLVYEANISLNWTDINKELTAKDTKWTEMTTEPSTIDTQYATKHSIKL
                     KKYSYILSQQPYTLFRNAFFQKPDSVKNNDESNELIFYGGNETMSIHSETQVVDFRGE
                     LPEKKENASVFSESFPYISKLGSSLGNLRYFDRSDNTIDYRIFVEGFPVFGTDSRGQI
                     GVEVGGETNGSVQVNIQTSLNTIQVPIPSDEEVELASTNEIIQQLKAKGADSNKIGSV
                     IVGYTWQNVKETNRVVDLLPEWYVKYKDNWYSANDLLEKLPGLEAN"
     gene            15661..16530
                     /locus_tag="ACG842_001529"
     CDS             15661..16530
                     /locus_tag="ACG842_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system regulatory protein YycI"
                     /protein_id="HFQ0248076.1"
                     /translation="MDFKKIEWIFFVAFLGLNVFLFSSYHDAVYQENNVIRSNQTESI
                     QQRLASDDITYTGKISSENKQGYYLSAEQTNLSTSLANYRKAGKSGLLASGVFLDGDV
                     LTKSLSSSDAEKNVIDPDKISSSLEDFLSNKDNILFGNDYVYTKNFSHLEEEPLEIQA
                     SQEYEGIPFNDDTAKIVLSLEKNDNDYSITKYMQTHLSDIEQLREKTELYTEEEAINT
                     LYINNKIPRGSKILWRQLAYSCILKVREKNVYVPVWHVAIETSDKVVQIESVNAFSNT
                     IVTNNTVPKVEDQ"
     gene            16585..17394
                     /locus_tag="ACG842_001530"
     CDS             16585..17394
                     /locus_tag="ACG842_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFQ0248077.1"
                     /translation="MAEEKPAFKISILASGSSGNSLYIESEKKRLLVDAGLSGKKITS
                     LMAQIDRTPDQLDGILVTHEHRDHIHGVGVLARKYHLDVFANQQTWEAMEPLIGNVPV
                     EQKHLFEMGKVRTFGDIDIESFGVSHDAAAPQFYRFHKENRSFVMLTDTGYCSDYMRG
                     VIENADGYLMESNHDLEMLRMGPYPWNLKQRILGDRGHLSNEDGALVMTDVIGNRTKR
                     IYLGHLSKENNMKELAHLTMENVLKEKDFGVGHDFEIYDTDPDEATELFSI"
     gene            complement(17497..18126)
                     /locus_tag="ACG842_001531"
     CDS             complement(17497..18126)
                     /locus_tag="ACG842_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HFQ0248078.1"
                     /translation="MKIALIGATGHAGSFILDEALSRDLDVTAIVRHPEKLPSDVPFI
                     QKDLFELTTTDLASFDVVVDAFNAPKGKEEMHQTSLSHLIEMLEGTDTRLIVVGGASS
                     LFLDTEKTTRMIDQVPKNAPFYPTAFNMYEGLLKLKQSKNLHWTYISPASLFDPHGKR
                     TGTYLLSDDYLQRNAASDSTISMADYAIALVDEILDEKHEKQHISVVSR"
     gene            18296..19588
                     /locus_tag="ACG842_001532"
     CDS             18296..19588
                     /locus_tag="ACG842_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFQ0248079.1"
                     /translation="MKEWLDRAFLPMITSVVNLRPLTALRNGMTYTIPIIIVGSLFFL
                     FAHFPVDYIAQWIDKTGFSMYFTQVYQATFDVIAFWIVFAIAYAYAKEEDIDRLPVGI
                     TALSAYLLLIQPLNTQIDTVWTGAEGILGAVTTGLIIGWGYARLVRLAKGWKQREKIP
                     ENVWQSFTSLIPIIVIVTFTLIGSAVFQHLFRMTVVQGVWTYVQLPIQSFIDTLFGVL
                     LLGFFVPFLWLFGLHGSAMVNGLVSPVLQANSLANAEILASGQELTVANGGHIVTQQF
                     LDQFMTVTGAGLTLGAVFFMTVFAKSKKYRELGNLALLPAFFNINESIIFSTPIVMNP
                     MMAVPFIFAPVVSGLITYSALYFGFVPLFTAVQVPWTTPPILSGFLVGGVPAAILQGI
                     VLSISFFIYFPFLKKIDSANCKKERQTKNDGAAEKMND"
     gene            complement(19664..20326)
                     /locus_tag="ACG842_001533"
     CDS             complement(19664..20326)
                     /locus_tag="ACG842_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HFQ0248080.1"
                     /translation="MSLKKYSFLSIFCYGLIFISPLILASIGLVKSTSELITATAVMY
                     ILGAVVLAIFYFKQREPLAIESAVKKASAPKIVIYGLVGIFVALILQSIAVMLESVLF
                     GKATPSENTQNIIQMIMEAPAFILATTVAGPIMEEFVFRRSILGIISRYSNFWVGAVI
                     SSLLFAFAHNDGHLLIYFFLGFFFSLEYKATGRIWTSMITHVGMNTLVVLVQLAVQKG
                     LV"
     gene            20551..20835
                     /gene="groES"
                     /locus_tag="ACG842_001534"
     CDS             20551..20835
                     /gene="groES"
                     /locus_tag="ACG842_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HFQ0248081.1"
                     /translation="MLKPLGDRVIIEVAKEEEKTVGGIVLASAAKEKPQTGTVVAVGE
                     GRLLENGEKVPAAVKAGDQVMFEKYAGTEVKYEGKEYLIVAGKDIMAIVE"
     gene            20886..22511
                     /gene="groL"
                     /locus_tag="ACG842_001535"
     CDS             20886..22511
                     /gene="groL"
                     /locus_tag="ACG842_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736685.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HFQ0248082.1"
                     /translation="MAKELKFAEDARAAMLRGVDKLADTVKVTLGPKGRNVVLEKSYG
                     SPLITNDGVTIAKEIELEDHFENMGAKLVSEVASKTNDIAGDGTTTATVLTQAIVREG
                     LKNVTAGANPLGIRRGIELATKAAVEELHNISTVVDSKEAIAQVAAVSSGSDKVGHLI
                     ADAMEKVGNDGVITIEESKGIETELDVVEGMQFDRGYLSQYMVTDNDKMEAVLENPYI
                     LITDKKISNIQDILPLLEQILQQSRPLLIIADDVDGEALPTLVLNKIRGTFNVVAVKA
                     PGFGDRRKAMLEDIAILTGGTVITDDLGLELKDATIENLGNASKVVVDKDNTTIVEGS
                     GEKEAIEARVQLIKNQIAETTSDFDREKLQERLAKLAGGVAVVKVGAATETELKELKL
                     RIEDALNATRAAVEEGMVSGGGTALVNVISKVSAVEAEGDVATGIKIVVRALEEPIRQ
                     IAENAGYEGSVIVDKLKNVELGTGFNAATGEWVNMVEAGIVDPTKVTRSALQNAASVS
                     ALLLTTEAVVADKPEPAAPAAPAMDPSMMGGMM"
BASE COUNT         7782 a         3605 c         4523 g         6811 t
ORIGIN      
        1 ttttgattag aaaaataggt atattgaatc agtgacataa aagtatttat agtttgtagg
       61 gggaatcgta atggaagaaa aaaatagact aaatgctaaa caatgttcag atcaagagct
      121 taaaaaaatt aaaggaggtg ctggaacaaa acctcaagga aaaccggcat caaatttagt
      181 ggaatgtgtt ttttcattat ttaaaaagtg taactaaaat tttttttatg atatcctaaa
      241 atcaggaggg gatctcttga ttactatcga tacaaaagct tatctattaa gaatgttcgt
      301 gggaaacttt ttcttttttc tcttatttta tatgactaat actgatctta acaaaagaaa
      361 atatcttttt gtatgtttat tgataccact cacttttttt gttgaattat ttactgatgt
      421 ttccgcagct atacctataa tcggaagtta ttttatatta aaggaaaaga agaaaactaa
      481 tatagtgctt ttaaataaat tactattatg tatgcttatt gatgatttca gatctattat
      541 tacttccatg gttatgacgt atatgttttc atttgaaggg ataaaagggt atggttatgt
      601 agtattccag ataatgcttg attctttgtt tttgctgatg tttatttggt tatatagaaa
      661 attagctata aataggattg ttgaacagtc cagttctaaa cttactgcta tattcatgat
      721 ttatttactt acaattggtt tatttgtttc ttatgttgct catcaatatc aagtatttga
      781 tcattttgtg tttggtgtct taatattttt aatcattcaa atgtttgttg ttctattttt
      841 atttattaga ataactatta ggcaacgaga acaatatgaa cagcaattga aaagaaaaga
      901 attggtttat ctaaaaaaat atactgattc actggaaaaa gatcaagaaa aatttgccag
      961 atttagacat gactataaaa atttattgct gagtttaaaa gaaatcgcta atcaaaacca
     1021 tgattcgata ttggaaaaag aaataaaaga attagagatc tattctaatt cttatctaac
     1081 caataagttt gaatatcgtt atttaaaaaa tataaaaaat gaatatttga aaagtttgtt
     1141 gatagcgaag ctataccaag caaataacaa taagatttgt tgtaaattcg aatgtccaag
     1201 tactattatt gaagttcctg ttccaatatt tgactgtatt agggtgcttg gaattattct
     1261 cgataatgca attgaagcat ctatagagag tcaagataga gaactgtctc tagctattta
     1321 ccgagatgat aaacagatag aaatttcgat atcgaatagt tgtagagaga tactactctc
     1381 tatagatact ctaatgaaaa aaggaataac aacaaaaaag ggacatcaag ggttaggttt
     1441 atctagtata gaggaaatca ataaaaagaa tcccaatatg tttgtgaact atcaaaacga
     1501 tccgtatcaa tttactactc aagttatttt gttgttttga gggaaggagg atttatgagt
     1561 tatccaataa tattatgtga agatcaaatt atacaattaa atcagttaga acgaattatt
     1621 gataatttca ttctttttca cgataaagta tttaagatag tattgaaaac acaaagtccg
     1681 ttagaagtta aaaaatacct caaacaattc cgaccaaagc aaggaatata tttcttagat
     1741 attgatttaa atcatgaagt taacggtata gaattagcag aagtaatcag aaaatatgat
     1801 gttcaagcaa aaatcatttt tacaactact catgatgaga tgttacccgt aacaataaaa
     1861 agaagagttg aaacgttagg atttgtaaca aaagatcaaa cactagatga gtatcgaaac
     1921 gagattgttg agttattgtt attagcgcaa gaaaggatag atgcaacaaa acaggttacg
     1981 aatcaagcat ttgttttttc cataggttct caaacattta cttttgatat aaatgacatt
     2041 tattttgtag aagcttcaaa attaccacat agattatcat tgtgtactaa agatgggcag
     2101 tacgaatttt atggaagaat tagtgagctt gaaaagaaat atcctatgtt aacaagaatt
     2161 agtcgagcgt gtttagtaaa tatctttaac gttaaagaaa tagattttaa aaaacgttca
     2221 ctatattttg attcagagtt ggccagaaac tttactttag ggaaagcaca aaaaattaag
     2281 gagaagttga aagaaagcac tgtttagtgg ttttttcttt agggagattg catttttcta
     2341 ataaaataga ttccttcact agtgcaaatt tttctgaatc ctattaaagt tgtaccaatt
     2401 tttatagctg ttattagtta cgtgcataac agcaatatta gttactggag gaatatttct
     2461 atggtctgga accaaaaaag ggccaaatga atagttcttt tcttgttttt attaagtacc
     2521 ataaagggga attaaaatta aatgtatggt ggaagatgcg aaagatttgg caaattctac
     2581 taatttttag aaagtgtttt tcatttttta gttaaaaaat ttgtcctgtc aagtgcaatc
     2641 ctatgattgt acttgatagg atttgagata attactttcc tgtttttata aatttaactt
     2701 gcaggaacag gctttaatgc acatccgaca agtacattac caatacctcc tgtaacggcc
     2761 gaaataggtc ctcctgcagc accaactaca gcgctgcaag tccttttgta aaaatatctc
     2821 taccattaca tttccaaaga atttttatct gactttaata aaagataaaa atttaatata
     2881 attataaaaa ctagcacaag aagctttagt aaaaagttct gtccattcca aacttttgaa
     2941 aagataaaga acatacctat gttcgtaatc gacacaaacc aatcttgagg tttgatattt
     3001 tttaattgtt tacgcaccgg tccacatgct gaatccagca cctaataaag ctccacctac
     3061 accacaagca gctattgcat aaggtcctcc ggctgaacaa agttttactc ctgctgttgc
     3121 cccagaaaaa gtagtgccta aaactcccac ggcactccca ccttgaatat ttttcaactc
     3181 ttctgaagat aatataaatg ttgtttttgt gtttttaata tgcatactac ttattctcct
     3241 tttttatttt tttcgagcat cccagccagc tgcagcacct ccagcgacag ctccatataa
     3301 ccctccaaca acaccgcaag ctggtccgca aattgctgtt cctaaacctg caccacctac
     3361 tgcccctgtg gtaagtccac ctacaacttg ccccaatttt ccccaagcat tattgcctcc
     3421 accaacagtt tgtgcatttc cacaatactt agttccttta cattttgcat gtttaaacac
     3481 gcttctttaa ataatatttt ttacggtgct actgtaacat acgtatttga ttaacagtaa
     3541 aaatcttcct gaaacttatc aagtttattc ttgaatagta aaatttccaa aataaatttg
     3601 attcaggaat gaaaaagtta gtgttcaaga actgttttca aagtttttgt ggaattatga
     3661 gacaatattt tcactgtatt aaagctatat tgagaatttt tctataaaaa ttttttagaa
     3721 ggagttgtgt tagataagtg ataaaatcat ttccttttgt gcagcaacaa gatgaaaaag
     3781 attgtggtgt tgcttgcctt tcgatgattt taaaatattt taaaactgat atcccgattc
     3841 ataaattacg ggacctatct gggactgatt tagagggtac ttcagcattt ggattgaaaa
     3901 agacatttga aaaattgtct tttgattgta tggctattca agcagatagt gagatctgga
     3961 aagaaagaga tcttatctta ccattaattg cacatatatt ggttgataac agctatatgc
     4021 actacgttgt aatttataaa acgaagggaa acgtactatg gattgcagat cccgctaatg
     4081 gaaaaaatag aaaaacgatt gaagcgtttc aaaaagaatg gacaggtatt cttttattac
     4141 ctatgcctag agaaacttat attcctatta aagaaaaagt agctggatta agttcttttt
     4201 tgcctataat ttggaaacaa aaaggattag tattccacat cgttttagca tcgattttca
     4261 ttacgttttt tggcatagga agctcatatt attttcaagg tattcttgat tttttcatcc
     4321 ctaatcaagc acgttcaact ttaaatataa tttccattgg cttgatcgct gtctatttat
     4381 ttcgggtatt gtttgaatat agtcgtagtt atcttcttat tattttagga cagcgaatga
     4441 gtatggcaat tatgcttcag tatttcaaac atgtgttaac tttaccaatg aatttttttg
     4501 ctacgagaaa atcaggggaa attatttctc gttttcttga tgccagtaag attattgatg
     4561 ctttagcaag tgctacgtta tcaatatttt tagatattgg tatggtgttg cttgttgggg
     4621 ttacgttagc tattcagaat agtacgttgt ttttaatcac tctatgctct ttgccttttt
     4681 atgtagttgc aatattcgct ttcgtgaaaa gctatgaaaa atcgaacagg gaagaaatgg
     4741 cagccggtgc agctgtaaat tctagtatca tagaaagtct taaaggaata gaaacaatta
     4801 aagcatatag cggtgaagag aaagtatata accgtataga ccatgaattt gttaaattaa
     4861 tgaaaaaatc atttcgtacg gttacgcttg ataatattca acaaggaatt aaacatttga
     4921 ttcaattaat tagcagtgca ttaattttat ggctaggttc ttattttgtt atggataata
     4981 gaattagtct aggtcaactt atcacataca atgctttact agtatttttg acagatcctt
     5041 tacaaaacat tattaattta caagtgaaaa tgcaaacggc tcaagttgca aataatcgtt
     5101 taaatgaaat tttcgcgatt gattcagaaa aatcgaacca gtcttttgaa tatatgcttt
     5161 ctgaacaatt atttcaacaa gatatcactg taaaaaacgt ttcattctcg tatagcatga
     5221 aggccccaac gttgaacgaa atttcatgta caattccttc gcacagtaaa acggctatag
     5281 ttggagttag tggttctgga aaatcaactc ttgcaaagtt atttgttcgt ttttatgaag
     5341 catcagaagg atcgatccat tatggaaaca ttaatcactt agacattcct tttcagatat
     5401 tacgcgatcg agtgacatat ttgcctcaag agtcattttt ctttagtgga acaatcattg
     5461 agaatcttat attcggttta gatagcccac caagtttcga acaaataata gaagtttgtg
     5521 agatagtcca actaaatgaa tttattaatc aacagccatt acgttacgag acattgttgg
     5581 aagaaggtgg aaataatctt tcaggagggc aacgacaacg attagcctta gcacgggctt
     5641 tattaaaaaa tccggacata ttaattttag atgaagcaac tagtggatta gataccttgt
     5701 tagaacatgc aattatgaat tatctcctca atttaaaaga aaaaacgatt atttttattg
     5761 cgcatcattt accaatagca aaagcatgtg atcaaatttt agttctccat gaagggcatt
     5821 tggtagaaaa aggaacgcac gatgaacttc gttttgccga tggtatctat caaaaattat
     5881 gggaaatcta ggagagggaa acaatttatg cagaataata attggttcga acattctagt
     5941 atctatacta aacaacatca tagcttctac cgttgggtgt tataccctgt aattcttttt
     6001 ttatgtatta ttggattgtt tttaaccttt gctaaaaaag aggttgttat tcggacaacg
     6061 gctcaattga accccgaaaa aatagagaag ttacaagtgc cattagaagc taaaataatg
     6121 gaaaacttcc taaaagaaaa tcaatttgtt cataaggacg atgtgttggt tagattggat
     6181 tgttctttaa tagagaatga aaaggctcag atagaacaag aaaaccaacg tattactcaa
     6241 caaataaaga tggctcagct atttattgaa agtataagta aaggaaaaaa tttgttttca
     6301 acggatgaca gttttggcta cagtaatcaa ttaaagagca tgttgtcaga aaaagaatca
     6361 ctccgctacg ctttgaagca aagtgaatta aatgatcaaa agcaattaga agtatacgaa
     6421 aagacaaaaa gacaactaga aaaacaaatt gagagttcag atagtaaatt acaagaatgg
     6481 caacaagtac aggtagcttg gagtaataat caatcattaa aagatttttc aaaagaaatg
     6541 atggcaaact atgagaattg gcaagaacaa ctaaataatg tttctgatga tcaaaaaaat
     6601 caagtgaaac tgacaatttc agcaagcata aatgaacaaa ttgagcaact aaaaaaagaa
     6661 gtagaacagt atcagtcaga aaaagctaaa ttagttaaac caactacttc tgagaatgac
     6721 agaattagtc aaacggaaaa aggaaagcaa gagctagaac aagcaataac aactgtaaaa
     6781 caaacagtta cagagctaaa tgaaaaacaa gagaagaacc agtcagttat caaaagtttg
     6841 gacgatcaac tttcaaaagg aatactaaaa gctcctgtat ctggaacggt tcatttaaat
     6901 gaagaaacaa agggacaagc agaactagcc aaaggaacag tgcttgcaga aatttatccg
     6961 atccacgaaa aatcaaaaat gaaatttaca gcacttttac ctgcgaatga aagtacctat
     7021 attaaagaag ggatgaaggt tcattttaaa ttggatcaaa aaggtggggc tccgattacg
     7081 atcgacggaa ctttagatga aatttcagaa aattctactg ccactgagaa aggtgttttt
     7141 tatgcagtga aaggaacgtt aaaaacaact caatcgtttc cgtttcgtta tggattaact
     7201 ggagagttgt ctttaattgt aggagaaaaa acatattggc aacaaattgt cgatgtgttt
     7261 ctaaataaaa aataattatt tttactaaga atataggagg agtttatatg aataaaagaa
     7321 gatggattgc tgtgggagta gcgtgcggat tattgttact atccattatc gtcgcgctaa
     7381 ttccaggaaa agataaagag gaagcatcaa atacaaccct aacaggaata aataagattt
     7441 tttacggatc taacgaaatc acagaagaaa cgttagaaga aggcgcatca aataaaaaga
     7501 ttgtcaaact atccgtcaat ggagttattg cagatacagg tgaaagcaat ctttttagta
     7561 gggaacaata taaccatcag aattttttaa cacagctaaa gaaaattcaa gaagacaaag
     7621 cggttaaggg agtgttatta gaagtaaatt cacctggggg aggaatatat gaaagtgctg
     7681 agattgctaa agaaatggca aaaattaaaa agctagatat tcctatctat actgcattta
     7741 aaaatacagc ggcaagtgga gggtactaca tttctgctgg ttcagataaa atatttgcta
     7801 cggaagaaac aacaactgga tcaattggtg tcattatttc tggtttaaat tattctggct
     7861 tattagaaaa acttggcgta actgatgcga cgtataaaag tggtgcatta aaagatatga
     7921 tgtcacctca acacaaacct tcagaggaag agaataaagt tatacaggaa tttgtgatga
     7981 gtgcttatga tcgttttgtc aatgttgtag caaaaggaag aaatatggat actaatgcag
     8041 tgaaagaact tgcggatgga agaatttatg atggtaacca agcagtagaa aatgggttgg
     8101 tggatcaaat tggttactct gaagatgcat tagattccct aaaaaaagag aagaaattaa
     8161 ctgatgcgac ggttatagaa tataaaaatg acacaactgg ctttgcttct tcttggttag
     8221 gtaataagat tgctgaatgg caagggctaa aggcaactac atcagatcgt gtaatttcag
     8281 tttttgaaaa acttggattt tctgatactc caaaaccaat gtattactat ggagggctat
     8341 aaaatgaata agaagagtgg cattgaaact ttgcctaatt atgtatttgc gggattttgg
     8401 ttacgatttt ttgcttttct tattgattta atttgtatta gcatcatcac ccatgctact
     8461 ataggtctct tttacaaaat catgggactg gaatctgaca aatccattat tactttttat
     8521 ggtatagcgt cattagctat ttatttaacc tattttgtac ttttgactaa gctgaattca
     8581 ggacaaacaa ttgggaaaat gatttttggg ataagagtag tgagctttga ggaaaaagag
     8641 ctaagttggc aaactatctt tattcgtgag tgtatatgta ggtttatatt aaaaaatttt
     8701 gtatttcaaa ttgggtattt ggtggctgct ttcacgccca agaaacaaca tattggggat
     8761 tatttttcta acacgtcagt ggttgttatt cgttcattag aagctattaa ctatggtcaa
     8821 aaaatttcta attctaaaac acttaatctg gagagaatta aatgaataag agaaaaacag
     8881 agtttatttt gattttaatt agtggaataa cagggatttt atgtagtctt ttagcattga
     8941 tgggtggtat gtttctatct tctattgatt caactacttt aaatactaca gaactaacca
     9001 gtaaggatat agatatgtta gtcaatctaa gcgggaatgt tgttttattt ggaggaatta
     9061 ccctagtttt ttcgataatg attttcataa taggtttttt agtaagaaaa aatcaaaaaa
     9121 cagtggtcta tggtattagt ttactcattt tatctattat tccatttttt cttttaacat
     9181 tattgtgggt aattcctgga gttttaggta ttattgcggg attgatgctt ctaataagaa
     9241 aatcttaatt aggcaatgtg tagacaacgt gaaacgatta atcaattaaa agctaaatac
     9301 gttttgagaa atgataactt gtagtcgatg gaagacatgt agataaaaaa actacgtgtc
     9361 ttttatttat tctaattttt tcgaaaaaaa gacactctct gattgttgta ttaatggaaa
     9421 aggggaaagc agcaagtaaa tttagttttt ctattaaata ttcttcactt tttaaccttt
     9481 tatagtactt agatttaatc ctatatgttt agagagttca ttggcagcat gattttgcaa
     9541 agcttctcga attattgtta tattagaaac tttactgttc ataattatgc ttctttttta
     9601 actcaaagtc caaagggcta cagtggactt tgagttaaaa ataaaaagcc ctagttattt
     9661 tgaaaaataa aaaaagaaca gcgtaaatgt tttcgaaatg ctttcgcacc aacatttacc
     9721 tgttccgtgg gtttggcacc caatgggccg tgaggggctc gaacccgcga cccgctgatt
     9781 aagagtcagc tgctctacca actgagctaa cggcccgtac aaaaagtact taataatagt
     9841 agcactaaga attttaaaat gcaagactac atttaaagaa aatcagaaaa atctttactt
     9901 ccttatagga aatcattcat agattattca catttgctcg ctattatata agcataccaa
     9961 caaacaaagt tcttgcaaga gaacaaaccc taacactttt ttcatttttt actcctttcc
    10021 cgccgtctcc ccggcgggtc tcctgttctt aacgataaaa gacaagaagg cttagccttc
    10081 ttgttttttg ttttcatgga atcccgctaa tcaaatcacc tagttaaagc aactagattg
    10141 aatgtttatc ataagtctga tatcatgttt atgaaatgaa aaaaggagcg gttatgatgg
    10201 aaaaaatgcg tcagcaatta atggagtggg ggaaggaact agaaacattc cgtctgcctc
    10261 actgggaaga actccctgac ttagaacttt atatggacca agtcagaacg ttagttgatc
    10321 gttatctttc acctgtgata caaggagaaa agcatccatt gcttacctct tctatggtaa
    10381 acaattacgt taaactaggt ttgataccag caccagttaa aaaaagatat aacaaagaac
    10441 acgtagcatt tttacttgca atcactacgt tgaaacaagt attgactatt cctgaaatca
    10501 aagaagggat attgttccaa ggaaaaacag taggaattcg tgaagcctat aatctatttt
    10561 gcgatgaaca agaagcagct gtgtggatgg tcagccaatt agcacaaggt aaatctcatc
    10621 cgcaaaaatt cgaccaggca acccctgtag aatatattgc tgtaagagcg gcaactttat
    10681 cgtttgcaat gaaacttctt gcggaaaaaa cgattgttct agaaaccgaa tatttaaaag
    10741 aggagaaaac aaatgaaaaa caataaaatc gcaattttag tagattcagg tacggatgtt
    10801 ccagaagaat tgataaaaaa atacgagatg tatatgatcc cattgaaaat tatttataaa
    10861 gatcaggtat atacagataa aattgacatt acccctgaag agatctatca acgtttgcca
    10921 gaggaaatcc ctggtacatc tttgccggat ggtgagtcga tcacagaaat ctttgagcaa
    10981 atcaaaaaag acggttatga aaaggtactt gcggtaacaa tttccagtgg attgagcggg
    11041 acatataatg tggttcgttt aattgcacaa cagcaagaag aattggaagc atatgtacta
    11101 gatacgaaaa atattggtat tggtgcaggt ttttctgcga tccaagcagc aaaatggctg
    11161 gaagaaggag tagaatggga tcaactgatc agtaatctga atgagttagt cgaaagaaca
    11221 aaagttttct ttaatgttgc gacattggaa tatcttcaaa aaggtggacg gatcggttta
    11281 gttgcttcta ttgtaggaac cgctttaaag cttaacccaa tcatctcatg taatgaagaa
    11341 gggatttatt acacagtagg aaaagctagg ggacgtaaaa aaagtttgga taaaacggtt
    11401 gcttacgtca aagaaaaaat cggtaatgca aaggtcttca acttagctgt tgcccatggg
    11461 gatgcgaaag aagaagcaat tgaaatggaa gcgagactta aaagagagtt cccacaagca
    11521 aaccaaatct actttggcca gatttctccg gcattagtag tccacactgg tccgggatta
    11581 ttgggtgtcg gtgtacaagt tttagaagga taaatcaaaa aaaggaaaca ctgaggtgaa
    11641 aatcagtgtt ttcctttttt attgaagtta gaacaaaaaa taataaagta ttttgcactt
    11701 tcattttaat tttctctttt gttttatagg caaagatgaa acaaaagcag aaatacggta
    11761 taatgagaag gtatgacaat aatatataag aggtggctct ttatgaaaaa agttttagtt
    11821 gtcgatgatg agaagccgat ttcggatatc gtcaaattca atttagccaa agaaggctat
    11881 gacgtttata ctgcatatga tggggaagaa gccttggaaa aagttgctga ggtagaacct
    11941 gatttgattt tacttgattt gatgctacct aaaatggacg gcttagaagt tgctcgtgag
    12001 gtacgcaaaa cttatgatat gccgatcatt atggtaacag ccaaagattc tgaaattgat
    12061 aaagtcctag gcttagagtt aggcgccgat gattacgtga ccaagccatt ttccaaccga
    12121 gaattagttg cacgtgtcaa agcaaatctt cgtcgcggtg ccacggctgc gaaggaacca
    12181 gaagaggcag cacctgcgga attgacgatt ggtgatttga cgatccatcc agaagcttac
    12241 atggtgacga aacgaggaga aacaatcgag ttaacacatc gtgaattcga actactcttt
    12301 tacttggcga agcaccttgg acaagtgatg acaagagaac atttattaca aacggtttgg
    12361 ggttatgatt attttggaga tgtacgaaca gtggatgtaa ctgtacgtcg tttgcgtgaa
    12421 aagatcgaag ataatccaag ccatccaaat tatttagtga cacgtcgtgg tgtcggttac
    12481 tatttgcgta acccagaaca ggagtaattg gatgaaaaga aaagtccgct tttttcgatc
    12541 agtcaatttt aagatcgcta ttacctttat tctgatttta ttgatctcga tcgaaattat
    12601 tggtgcctac tttatacgtg gtttagaacg ttccacgatc aatacattta ttaaagatat
    12661 gaatcaaacg gtggaatcat tagcaacaac aatcagcccg gaattgaatc gaaaagataa
    12721 tgcagatgat gaagaggtaa atgcgaatat caaaagattt attgaaaaca gtgcgactag
    12781 cgatatcatc gaaatacggg tcgttgacga aaaagggatc atccgaggaa caaccgatgt
    12841 caatgaacaa agcgctgtgg gaaagaaaaa tgattatgtt gatatcaatg atttcacgac
    12901 taaacgttat gttgctttag ataacgacga taaacgggtg aatatcaatg ttcaaccgat
    12961 cctgtccccg accggcgatg ccgttatcgg ggctctttac gtcaaaagta atatcgaaca
    13021 aaaatattcg gaaatcaata acacagcagt cattttcttt actgcctcac taattgcggc
    13081 atttatttca atgatcgtat ctgttttagt tgctcgttcg attacacagc cgatcggtga
    13141 aatgagagaa caagccattc gaatcgctag aggagactac agtcggaaag tcaaagtgca
    13201 tggacaagat gaacttggac agctggctga taccttcaat cagttagctg aacgtattga
    13261 agaagctcaa gatacaatgg aagctgaacg caatcgtttg gatagcgtgc tatcccatat
    13321 gacagacggc gtcattgcga cagatcgcag gggtaaagtc attacgatca atgatatggc
    13381 tgtttccttg ctagatatca aaaacgagga agcaatcgga caatcgattt tgacactttt
    13441 agatatcgaa gaagaatata cgttgcggaa attattagaa gatccaaacg aaatggtact
    13501 tgaccgttca accagtacat tggaaagtga tcagatgatc ttgcgagtag acttttctat
    13561 gatccgtcgc gaatcaggtt ttatcagcgg attagtagct gtcttgcatg atgtgacaga
    13621 acaagaaaaa acagaacggg aacgtcgtga attcgtctct aatgtttccc atgaacttcg
    13681 tacaccgttg acaagtatgc gtagttatat cgaagcattg agcgaaggtg cgtggaaaga
    13741 cgaggaagta gcaccaaatt ttttgaaggt aacgctagat gaaacagacc gaatgattcg
    13801 aatgatcaat gacctcttaa atctatctcg catggataca ggtaataccc agcttcaatt
    13861 agaatatgtc aatttcaacg agttagtgaa ttttgttctt gaccggtttg atatgatggt
    13921 gggaaatcag gaaaaaaatt ataagatccg tcgtgaattt acgcaaagag atctttgggt
    13981 cgaagtagat acagataaaa tcatccaagt agtcgataat atcatgaata acgcgatcaa
    14041 gtattcacca gatgggggaa cgattacttg tcgcttatta gaaacgcata ataacgtgat
    14101 tttaagtatc accgatcaag gattggggat tcctaaaaaa gatctgaacc gagtgttcga
    14161 gcggttctat cgagtagata aagcacgtgc aagagcacaa ggtgggacag gacttggatt
    14221 agctatttct agagaagtga ttaaggcgca tcgaggcgct atttgggcag agagcaagga
    14281 aggaaaaggg tcgacttttt atatctcgct gccatatgaa ccttatgagg aggaatggtg
    14341 ggaatgagaa tctctgaaaa agtcgttcgt ataggactca ttttgatggt tgcgttaagc
    14401 atatacttct cttatgcaat ctggctgagc ccagcaggaa aaagttcaat caacgtagat
    14461 gagacaaaca ctcaaatgat cgagtctcaa agcaatcgaa aaacttccga agtctttctt
    14521 ccgcttcatg ttacatggca ccattcggga atgatccaag aaaccaacag tgaaaatctc
    14581 gtgtaccgat tgcaattgat catcgataaa gcaagatttg gaaagctttc tgaagtagtt
    14641 tcaggagaca aatcacagtt tgaacaggtg aaaaatctga actctggttt tgagttggcc
    14701 tataatgcgc cttttttgct aagagaatac aaggaagcat ttgatttaga tttagatgtg
    14761 tcgaacttgg caaaagaaag cgaaaatatg tattttacta gaatacaatt cgatcaagga
    14821 aataaaaaaa tccgattttt gaattattcg aaccatctcg tatatgaagc aaatatttcg
    14881 ttgaattgga cagatatcaa taaagaatta acagcaaaag acacaaaatg gacagaaatg
    14941 acaacagaac catcgacgat cgacacgcaa tatgctacga aacattcgat caaactgaag
    15001 aagtatagtt atatcctgtc acagcaaccc tatacacttt ttagaaatgc ttttttccag
    15061 aaaccagata gcgtgaaaaa taatgatgaa agcaatgaat tgatttttta cggtgggaat
    15121 gaaacaatgt ctatccattc ggaaacgcaa gtagttgatt ttcgtggaga gctgccagag
    15181 aaaaaagaaa atgctagtgt attttctgaa agtttcccct atattagcaa attaggcagt
    15241 tctttaggga acctcagata ttttgaccgc agtgacaata cgatcgatta tcggatattc
    15301 gtggaaggct tcccggtttt tgggacagac agcagaggac agattggtgt ggaagttggc
    15361 ggtgaaacga atggttctgt gcaagtgaat atccaaacca gtttgaatac gattcaagtg
    15421 ccgatacctt cagacgaaga agtagaactt gcaagtacga atgaaatcat ccagcagtta
    15481 aaagctaagg gagcagatag caacaagatt ggttcagtga tcgttggcta tacatggcaa
    15541 aatgtcaaag aaacgaatcg tgtcgttgat ctcttacctg aatggtatgt gaaatataaa
    15601 gataactggt attcagctaa cgaccttctg gaaaaattac ctggattgga ggcgaattaa
    15661 ttggatttca aaaaaatcga atggatattt ttcgtcgctt ttttagggtt gaatgtgttt
    15721 ttattcagca gttatcatga cgcggtatac caagaaaaca atgtcattcg ctctaatcag
    15781 actgaatcaa tccaacaacg tctggctagc gacgatatca cttacacggg gaagatttcg
    15841 tctgagaaca agcaaggcta ttatcttagc gcggagcaga ccaatcttag cacttcatta
    15901 gcgaattatc gcaaggctgg taaaagcggc ttgctggcaa gtggtgtgtt tttagacggt
    15961 gatgttttga cgaaatcttt atccagttca gatgctgaaa agaacgtgat cgatccagat
    16021 aaaattagta gttccttgga agatttttta tctaacaaag acaatatttt attcggtaat
    16081 gattatgtgt acacgaaaaa cttttctcat ttagaagaag aaccactaga aatccaagca
    16141 agccaagagt atgaaggcat cccattcaat gatgatacag caaaaatcgt tctttcttta
    16201 gaaaaaaacg ataatgatta tagcatcacg aaatacatgc agacgcattt atctgatatc
    16261 gagcagctgc gcgaaaagac ggaactgtat acggaagagg aagcaatcaa tacattgtac
    16321 atcaataaca aaataccaag aggatcaaaa attttatggc gtcagctagc ttattcctgt
    16381 attctgaaag tccgggaaaa aaatgtgtat gttcctgttt ggcatgtagc gatcgaaaca
    16441 agcgataaag tggtccaaat tgagagcgtc aacgctttca gcaatacgat cgtgacaaac
    16501 aatactgtac caaaagtgga agatcaataa aaaagcgagt ataatgaaaa aagtttacaa
    16561 agtaagaagg gaagtaggag tcaaatggct gaagaaaaac cagcttttaa aatcagtatc
    16621 ctagcaagtg gaagttcggg taattcactt tatatcgaaa gtgaaaaaaa acggcttttg
    16681 gtcgatgcag gacttagcgg taaaaaaatc acttccttga tggctcaaat tgatcggaca
    16741 ccggaccaat tagatggaat acttgtgacg catgaacatc gagatcatat acatggcgtg
    16801 ggtgtcttgg ctcgtaagta tcatttggat gtttttgcca atcagcagac ttgggaagcg
    16861 atggaaccac tgattggaaa tgtaccagtg gaacaaaaac atctatttga gatggggaaa
    16921 gtcagaacat tcggcgatat cgatattgag agtttcggcg tctcccatga cgcagcggct
    16981 ccgcaatttt atcgtttcca taaagaaaat cggtcattcg tcatgttgac ggacaccgga
    17041 tattgcagtg attatatgcg aggtgtcatt gaaaatgccg atggttattt gatggaaagt
    17101 aatcatgatc ttgagatgtt acgcatggga ccttatccgt ggaatttaaa acagcgtatt
    17161 ttaggagata gaggacatct ttccaacgaa gacggtgcac ttgtcatgac tgatgtgatc
    17221 ggtaatcgga ccaaacgaat ctatcttggc catcttagca aggaaaacaa tatgaaagaa
    17281 cttgctcatt taacaatgga aaatgttttg aaagaaaaag attttggcgt tggacatgat
    17341 tttgaaatct atgatacaga tccagatgaa gcaactgagc tattttcgat ttaaacagtt
    17401 caatcaataa aaatataaaa agcagaagat aaaatcaatc agggttggtt ttatcttcta
    17461 tttttatttt tttagcatat taaaaaaata accgtattat cgactgacta cactgatgtg
    17521 ctgcttttca tgtttctcat ccagtatctc atctactaat gcaatcgcgt aatctgccat
    17581 gctgatcgta ctgtcgctag cagcgtttct ttgtaaataa tcatcactta acaaataagt
    17641 acctgtacgt ttgccatgtg gatcaaataa agaagctgga ctgatataag tccaatgtag
    17701 atttttagat tgtttcaatt ttaacaatcc ttcgtacata ttgaaagctg ttggatagaa
    17761 gggtgcattt ttaggtactt ggtcaatcat acgagtcgtc ttttctgtat ctaagaataa
    17821 cgaagaggca cccccaacga caattaagcg tgtatcggtt ccttcaagca tctcaatcaa
    17881 atgggacaaa gaagtttgat gcatttcttc ttttcctttg ggagcgttga aagcatcaac
    17941 gacaacatca aaagatgcaa gatcagtagt tgtcaactca aataaatcct tttgtataaa
    18001 cggaacatct gatggcaatt tttctggatg acgcacgatt gccgtcacgt ctaaatctct
    18061 ggacaatgct tcgtctaaaa taaatgaacc tgcatggcca gtagctccaa tgagtgcaat
    18121 tttcataaaa aacctccttt tttattcctg tccattgtaa gcagttgctg aatattcgtc
    18181 aaagaatccg atttttgaaa agctcagtgc gcattccttt catggttcgt gatcattggg
    18241 tatagttaaa ttaagaaaac tgattgataa aaatagaagt tgaaggagat aagaaatgaa
    18301 agagtggtta gatcgtgcct ttttaccaat gataacatct gttgtgaatc ttagaccctt
    18361 aactgctttg cgtaatggaa tgacttacac gatcccaata attattgttg gctcgctgtt
    18421 tttcttattt gctcattttc ctgtggatta catcgctcag tggatagaca aaacaggatt
    18481 ttctatgtat tttacacaag tataccaggc tacatttgat gtgatagcct tttggatcgt
    18541 ttttgccatt gcatacgctt atgcaaaaga agaagacatc gatcgcttac cagtcggcat
    18601 aacagccttg tctgcttatc tactgcttat tcagccacta aatactcaga tagatactgt
    18661 ctggacaggt gctgaaggga tactaggagc agttacaact gggctcatca tcggatgggg
    18721 ctatgcacgt ttagtaagac ttgcgaaagg gtggaagcaa agagagaaga tacctgaaaa
    18781 tgtttggcag tcttttactt cattgattcc aatcattgtt atagtcactt ttacgcttat
    18841 cggttcagcg gtttttcaac atttgttcag aatgactgtt gttcaaggag tatggacata
    18901 tgtccagctt cccattcaat cgttcataga cacgttgttt ggtgtgcttt tattaggttt
    18961 ttttgttccg tttttatggt tatttggtct tcatggatct gcgatggtca atgggttggt
    19021 tagtcccgtc ttgcaagcga attcattagc aaatgcagaa atcttagcct caggacaaga
    19081 gttgacggtg gcaaatggcg gacatatcgt tactcagcaa tttctcgatc agttcatgac
    19141 ggttacgggt gctggattga cgttaggggc tgtatttttt atgacggttt ttgcgaaatc
    19201 gaagaaatac agagaattag gaaatcttgc tcttttacca gcctttttca acatcaatga
    19261 atcaattatt ttttccacac cgatcgtgat gaacccgatg atggctgttc cttttatttt
    19321 cgcaccggtc gtttcaggat tgattaccta ttccgcttta tattttggat tcgttccttt
    19381 atttacagca gtccaagtcc cttggacaac accgccgatc ctctcgggtt tcttagtagg
    19441 cggagtgcct gcagcgattt tacaaggtat cgtgttgtca attagttttt tcatttattt
    19501 cccatttttg aaaaaaatag attctgcaaa ctgcaaaaag gaaagacaga caaaaaatga
    19561 tggagcagca gaaaagatga acgactaaaa aacaaaaata ctcctgtcgc tttagattgg
    19621 gaaaatcacc aatctaggat gacaggagta tttatagtca tgtttatact agcccttttt
    19681 ggacagctaa ttgaacaaga acaactaatg tgttcattcc tacgtgtgtg atcatcgatg
    19741 tccagatccg accagttgct ttgtactcta ggctgaagaa gaaacctagg aaaaagtaga
    19801 ttagcagatg accatcatta tgagcaaacg caaacagcaa agagctgatc actgctccga
    19861 cccaaaaatt tgaatatcga ctgatgatcc ccagtatact gcggcggaat acgaactctt
    19921 ccatgatggg tccagcaaca gttgtcgcca aaataaaagc gggtgcttcc atgatcatct
    19981 ggatgatatt ttgtgtattc tcggaagggg ttgctttgcc aaacaataca gattctaaca
    20041 tgacagcgat gctttgcaga atcaatgcga caaagattcc aaccaatcca taaataacaa
    20101 tctttggagc agaagctttt tttaccgctg attcgatcgc taagggttca cgttgcttaa
    20161 aataaaaaat agctaggaca acggcgccta gtatatacat gactgcagtt gctgtgatca
    20221 attcagacgt agattttaca agtcctatcg aagctaggat caagggtgag ataaaaatta
    20281 gaccatagca aaaaatgctg aggaagctat atttttttaa agacatgtaa ttcctccttt
    20341 taagggataa caagcatgca aggcaagctt atatcgattg atttcaatta tatcataatt
    20401 tcaataagca gctgtcagaa taaaatttct tttctttgat ttaaaagctt gcaaaaaaga
    20461 gggagaatga gtattattaa aaatgtaggt tagcactcga aggttaagag tgctaacaga
    20521 ttaaaaaaag agttatcgga gggatttatc gtgttaaaac cattaggtga tcgcgtcatt
    20581 atcgaagtcg caaaagaaga agaaaaaact gtcggaggaa tcgttttagc ttctgctgct
    20641 aaagagaaac cccaaactgg gacagttgtt gcagtcggtg aaggccgttt gttagaaaac
    20701 ggtgaaaaag ttcctgccgc tgtaaaagca ggcgaccaag ttatgttcga aaaatacgcc
    20761 ggtacagaag taaaatacga aggaaaagaa tatctgatcg tcgcaggaaa agatatcatg
    20821 gcgattgttg agtaaatttg gaagataaaa aaataatcaa aaataatcaa tgaggtgaat
    20881 caatcatggc aaaagaattg aaatttgcag aagacgcacg cgcagctatg ttacgcgggg
    20941 tagataaatt agcagataca gtgaaagtaa cattaggacc taaaggccga aatgtcgtac
    21001 tagaaaaatc ttacggctct ccattgatca caaatgatgg tgtgacgatc gcaaaagaaa
    21061 tcgaattaga agatcatttt gaaaacatgg gtgcaaaact tgtatcagaa gtagcatcaa
    21121 aaacaaatga tattgcagga gacggtacaa cgacagcaac tgttctgaca caagcaatcg
    21181 ttcgcgaagg tttaaaaaac gtaactgcag gtgctaatcc tctaggcatt cgtcgcggga
    21241 tcgaattggc aacaaaagca gcagtcgaag aattgcataa tatctcaact gttgttgatt
    21301 caaaagaagc cattgcacaa gtagcagctg tttcttcggg ttctgataaa gttggacatt
    21361 tgattgctga tgcaatggaa aaagttggaa acgacggtgt catcacaatc gaagaatcaa
    21421 aagggatcga aacagaacta gatgttgtag aaggtatgca gtttgatcgt ggttacttat
    21481 cacaatatat ggtaacagat aacgacaaaa tggaagctgt tctagaaaac ccatatatct
    21541 tgatcacaga caaaaaaatc tctaacatcc aagatattct gccattgtta gaacaaatct
    21601 tgcaacaatc acgtccattg ttgattattg ctgacgatgt agatggtgaa gcattgccaa
    21661 ctcttgtatt gaacaaaatc cgtggaacat tcaacgtagt agctgtaaaa gctcctggtt
    21721 tcggcgatcg tcgtaaagca atgcttgaag atatcgctat tttgacaggt ggtacagtaa
    21781 ttactgacga tctaggttta gaattgaaag atgcaacaat cgaaaactta ggaaatgctt
    21841 ctaaagttgt tgtagataaa gacaatacaa cgatcgtaga aggttctgga gaaaaagaag
    21901 caatcgaagc tcgtgtgcaa ttgatcaaaa accaaattgc tgaaacaact tctgattttg
    21961 accgcgaaaa attacaagaa cgcttagcta aattagcagg tggtgtagct gttgttaaag
    22021 ttggtgcagc tactgaaaca gaattgaaag aattaaaatt acgcattgaa gatgcattga
    22081 atgctacacg tgccgcagta gaagaaggaa tggtctcagg tggtggtaca gctctagtga
    22141 acgttatcag caaagtttct gctgtagaag cagaaggaga cgtggcaaca ggtatcaaga
    22201 tcgttgttcg tgctttagaa gaaccaattc gtcaaatcgc tgaaaacgct ggttatgaag
    22261 gatcagtgat tgttgataaa ttgaagaacg tggaactagg cactggtttc aatgctgcta
    22321 ctggagaatg ggtaaacatg gttgaagcag gtatcgtaga cccaactaaa gtaacacgtt
    22381 ctgccttgca aaatgccgct tccgtttctg ccttgttatt aacgacagaa gcagtcgttg
    22441 cagataaacc agaaccagca gcgccagcag ctcctgcaat ggatccatca atgatgggcg
    22501 gtatgatgta agaaaaagca acttgcttat ctacaactga tcattagtga aagacagata
    22561 aaaagaggct ggaacagaag cgtttaactc caagaaataa gaagaaattc acggaaattg
    22621 cttttcaaat ttttgtgaat ttcagcttat ttccgaagga gttgcttctg cttccgctgt
    22681 ttatacgtgt ttagagcgtg agacaaaagt gttctttact t
//
LOCUS       DAZKLM010000044        22719 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3764
            SAMEA115706913-rid22582563.denovo.044, whole genome shotgun
            sequence.
ACCESSION   DAZKLM010000044 DAZKLM010000000
VERSION     DAZKLM010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706913
            Sequence Read Archive: ERR13196647
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22719)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22719)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287823.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287823.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,004
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,849
            CDSs (with protein)               :: 2,849
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 41 of 96
            Pseudo Genes (incomplete)         :: 52 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 15 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22719
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706913-rid22582563.denovo.044"
                     /isolate="VRE3764"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(11..1942)
                     /locus_tag="ACG842_001536"
     CDS             complement(11..1942)
                     /locus_tag="ACG842_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-specific PTS transporter subunit EIIC"
                     /protein_id="HFQ0248083.1"
                     /translation="MNIKDLMVKEAMIMDLQATDKKGAIDEMVQKMFDAGRISDIETY
                     KEGILAREAQTSTGLGDGIAMPHAKNSAVKEATVLFAKSQKGVDYEALDGQPTYLFFM
                     IAAPEGANDTHLQALAALSRLLIDADFVGKLKEANTPEEVQDLFQTAEEQKEAEEKAE
                     QETQAAEASAHPERKFVVAVTACPTGIAHTYMAEDALKKKAKEMGVEIKVETNGSEGI
                     KNRLTAEDIARAEGVIVAADKKVEMNRFDGKELINRPVSDGIRKPEELINLALSGTAP
                     VFHGDGKESSSEEGNADGTIGQRIYKDLMNGVSHMLPFVIGGGIAIALSFMIDQFIGV
                     PHDQLSNLGNYNQAASWFNQIGQAAFGFMLPVLAGFIASSIGDRPGLIVGFAAGALAN
                     TGGAGFLGALIGGFLAGYVIVFLRKLFKNLPKSLDGIKTILFYPVFGLLITGFLMLLV
                     NVPMKAINDALNGFLTSMSGSNAALLGALLAGMMAADLGGPINKAAYVFGTATLATTV
                     ATGGSVVMASVMAGGMVPPLAIFVATRLFKNKFSKTDQDAGLTNIVMGLSFVTEGSIP
                     FAAADPIRAIPSFIIGSALTGGLVGAFGIKLLAPHGGIFVVFLLSHPVMYLVFIAIGA
                     VVSGVIYGLLRKAPAVAEA"
     gene            complement(1960..2874)
                     /gene="pfkB"
                     /locus_tag="ACG842_001537"
     CDS             complement(1960..2874)
                     /gene="pfkB"
                     /locus_tag="ACG842_001537"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008662 - 1-phosphofructokinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HFQ0248084.1"
                     /translation="MIYTVTLNPSIDYIVRVDGLKLGDLNRMKEDFKLPGGKGINVSR
                     ILKRIDSQSTALGFLGGFTGEFISKWLEKEQIQSLFTTVKEDTRINIKLKSDTETEIN
                     GLGPSISDEEIDELKQALGTVGQGDIVVLSGSAPASLRKGFYQELIDIIREKGADFVI
                     DTTGDDLKNALVKKPLLIKPNNHELAELYNTTFQSVEEIIPYGKKLLAEGAQNVLISM
                     AGDGALLFTEQGTYKTNVLVRPLKNSVGAGDSMIAGFIGAYSRTKDPIEAFKWGVACG
                     SATAFSDDLAARELIDELLPEVEITKID"
     gene            complement(2871..3623)
                     /locus_tag="ACG842_001538"
     CDS             complement(2871..3623)
                     /locus_tag="ACG842_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFQ0248085.1"
                     /translation="MLTEERRQKILDLLEQQKIVKSQDLVNLLNASESTIRRDLQELE
                     NSGFLERVHGGAKKEQLLNFEQNMSEKSLKNVHEKQQIAKSAAETINDEDVIYLDAGS
                     TTLEMIPFLQGKQITVVTNSVQHAANLVDLELATIILGGTIKLATNAVLGSNAIEQLK
                     NFHFNKAFMGMNGAHLEQGFTTPDPEEAAVKRLAMKNSEETFVLIDHTKFNQLSFTSV
                     APLEAATIITERCPLEFVNEFREKTTIKEAIQ"
     gene            complement(3782..3991)
                     /locus_tag="ACG842_001539"
     CDS             complement(3782..3991)
                     /locus_tag="ACG842_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0248086.1"
                     /translation="MDKQLYYRIMVPASLLSVCLFFVPNFPIRSVIGFLVMFGGMGLY
                     YYLLHKERKPASVYMKKTRSENKKR"
     gene            complement(4002..5330)
                     /locus_tag="ACG842_001540"
     CDS             complement(4002..5330)
                     /locus_tag="ACG842_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0248087.1"
                     /translation="MIGEDKLLMTIQLTDLNEEEKKQEISDWAILTRLLIQPTFIRSF
                     TQQADPYDLRKLQEKIMLASVRDESWLVVGNDENECSFRLEDNQLLIKNVLSISVFKE
                     KQFLIRDFIQKKMIAHGVFAYMRAYSEFIYHNTKQISQRLLFEKKDEIEHLPKMKQQN
                     GEVVVDCNQFPGYDLFYQGLCFTSCWEMYYSRYYHQIIPKPIFLDIQQVEKVKELENE
                     VICIQLYRDPFNWQKPNNQMFQSYFRDQLGFDHLAWDNGVGLLKPPFVEYIYTDHTIQ
                     SVQYQNAQMQPVPKKNASFFVTKSYDIQQGDYKERRVRGTLNAQAYFPWVDENRSRMM
                     CYKVIDPTVALDNGIEAYCYYIREYLEVEVTDEKYQEYLLSLRIYVPSESLSELPLKE
                     IKHRLSDVTFKRIKKKRRSIQFDVKKGEQHLRVQFLDYRELEQLITLQKI"
     gene            5466..5621
                     /locus_tag="ACG842_001541"
     CDS             5466..5621
                     /locus_tag="ACG842_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPJ_0845 family protein"
                     /protein_id="HFQ0248088.1"
                     /translation="MGLKFERTDNLDKLFESFAVDPEKTVAPKEEIDRFLKPQPQNEK
                     KKDKKQK"
     gene            complement(5695..6282)
                     /gene="yihA"
                     /locus_tag="ACG842_001542"
     CDS             complement(5695..6282)
                     /gene="yihA"
                     /locus_tag="ACG842_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HFQ0248089.1"
                     /translation="MKVHQAEIIISAVAPKQYPDTQLPEIALAGRSNVGKSSFINTLI
                     DRKNLARTSGKPGKTQTLNFYLIENALHFVDVPGYGYAKVSKTERAKWGKMIETYLTT
                     RDQLRAVVSLVDLRHKPSQEDIQMYQFLKYYDIPVIVVATKADKIPRGKWNKHESEIK
                     KALDFDRNDTFILFSSVTKQGKEEAWAAIEQAIES"
     gene            complement(6365..7615)
                     /gene="clpX"
                     /locus_tag="ACG842_001543"
     CDS             complement(6365..7615)
                     /gene="clpX"
                     /locus_tag="ACG842_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HFQ0248090.1"
                     /translation="MYDNPSSNETVRCSFCGKTQEEVKKIVAGPGVYICNECIDLCKE
                     IIDEEFYEEAVREFTEVPKPLEILEVLNNYVIGQDRAKRSLAVAVYNHYKRVNQQAQE
                     NTDDVELQKSNICLIGPTGSGKTFLAQTLAKTLNVPFAIADATSLTEAGYVGEDVENI
                     LLKLLQSADYNVELAEKGIIYIDEIDKIARKSENVSITRDVSGEGVQQALLKILEGTV
                     ASVPPQGGRKHPHQEFIQIDTTNILFIVGGAFDGIETIVKNRMGEKTIGFGTQNQKLS
                     EDESVMQHIIPEDLLKFGLIPEFIGRLPVMAALEKLTTDDLVRILTEPKNALVKQYQK
                     LLSLDNTELEFEPEALRAIAKKAIERNTGARGLRSIIEEIMMDVMFDIPSNENIEKVI
                     ITKEAAELSGEPTVIYKDSEKKAG"
     gene            complement(7862..9151)
                     /gene="tig"
                     /locus_tag="ACG842_001544"
     CDS             complement(7862..9151)
                     /gene="tig"
                     /locus_tag="ACG842_001544"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HFQ0248091.1"
                     /translation="MTATFEKKGTNDGVLKFTIAQDEIQKGLTTAFNKVKKSLNVPGF
                     RKGKVSRQVFNRMYGEEALYEDALNAVLPEAYEAAVKEAGIDPVSQPKIDVESMEKGQ
                     DWVITAEVTVKPEVKLGQYKDLVVEKQDREVTDADVDARIKREQEAQAELVIKEDAAA
                     ENGDTVVIDFEGFVDGEAFDGGKGENYSLELGSGSFIPGFEDQLVGHKSGESLDVNVT
                     FPEDYQAEDLAGKEAVFKVTIHEVKAKEVPELDDEFAKDVDDEVSTLDELKEKYRKEL
                     VETKEKAADDAKDEAAIRMAVENAEIVELPHVMVHDEVHRSMDEFLNNMQRQGISPEM
                     YYQLTGSTEEDLHKQFEGEAEMRTRTNLVIEAIAAAENLQATEEEINAEIQELSETYN
                     MPIEQIKRVLTEDMLEHDVTMKKAIELITGTATEAKE"
     gene            complement(9305..10237)
                     /locus_tag="ACG842_001545"
     CDS             complement(9305..10237)
                     /locus_tag="ACG842_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0248092.1"
                     /translation="MKPIDFPEKYENLMRVAQQALANQQYQQAKELFQRAYELKESFE
                     ANSLLVFCLYELDEKKEALKQALLHEKQYLQNEEFAEFYFDLLISTQDFLYARKLIAS
                     TDFYEAFEQRVIEKIQFAEELSGQMERQKIKALHNKSEELPSLEPARQLASIDEIEQL
                     PYHEFIQTASKLIVSPEVHILARAKLLETLRQLNECHQVFYLTIEEKLVKVIPKDLPK
                     PQQQASYQQLCVFSDRYGNEDALLSSVLKEEFTLQSAIVYPVYDTYIEDPKRWFQLTV
                     EACTGKTMYEGTEDEKQDFLKKREKILQQMIFFH"
     gene            complement(10394..11053)
                     /locus_tag="ACG842_001546"
     CDS             complement(10394..11053)
                     /locus_tag="ACG842_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HFQ0248093.1"
                     /translation="MSELSNENKEQEKDKQQQPAVGPDSNFSQKVLSSFEREPLTPEE
                     IKKKQRKWKKYTDTRNEPPKANDFPKYFYAGFFIRMFAFLVDLLCIGAITRLTLGIAK
                     NLGWLNFSDSYLSLYGFTALLIYLAYFILLTKFNHGQTIGKMIFGIRVISFDEEELSW
                     STVLVREGACRFILKFPLLMLGYLPAVFNQKKQHLGDFFTNTSVVTINLIKAFNQEVN
                     S"
     gene            complement(11056..12081)
                     /gene="sppA"
                     /locus_tag="ACG842_001547"
     CDS             complement(11056..12081)
                     /gene="sppA"
                     /locus_tag="ACG842_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HFQ0248094.1"
                     /translation="MNKRRWAAVLIAAGLFVVSLVSASLTKPQENDQIEGLNSWLYGD
                     EELAPNVLEEGDSTQKIVKLSVDGTIAAGGSSGLFASEGYNHKNFMEQLKAIEEDPTV
                     KGIFLEVNSPGGGVYESAEIAKKLDTIRKEHDIPMYVSMKNMAASGGYYISAQADKIF
                     ATEETVTGSIGVIMSGLNYSGLLEKLGVEDTTVKSGALKDMGSGTRPETKEEEAVLQA
                     YIDSAYSRFVAIVSEGRNKSEEEVKKIADGRIYDGVQAKEVGLVDELGFPEDALKAIR
                     KEQQLEDAELVEYSSTPSGFASTWLGSKLAEFQGLKPSETSQILSVLENLGTAESPKA
                     MYYYGGE"
     gene            complement(12094..12384)
                     /locus_tag="ACG842_001548"
     CDS             complement(12094..12384)
                     /locus_tag="ACG842_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0248095.1"
                     /translation="MDSKIEIMTLGMLKKQLSKFEASAGVSDDTKIFLDTGWDSIQEI
                     APDALEVAQAREFTVEDELTKESFSGYAREEKAERFDTSEQSETVIVIKNLY"
     gene            12793..14598
                     /gene="glmS"
                     /locus_tag="ACG842_001549"
     CDS             12793..14598
                     /gene="glmS"
                     /locus_tag="ACG842_001549"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:1901137 - carbohydrate derivative
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HFQ0248096.1"
                     /translation="MCGIVGMIGLKNVTPGLIDGLEKLEYRGYDSAGIFVSDGTTDYL
                     VKAQGRIQNLKNKINIDTTGNIGIGHTRWATHGQPSEENAHPHTSQSGRFVLVHNGVI
                     ENFEELKIAYLANDHFIGETDTEIIAHLIETFAKDTTTQEAFLKALRVIKGSYAFALI
                     DRTAPDVIYVAKNKSPLLIGLGDGFNVIASDAMAMLAHTKEFVEIEDEEMVTVTSEKV
                     IIQNFAGDIVERSSFEAQVDASDIEKGTYPFYMLKEIDEQPIIMRRIAQKYVTEDNRV
                     SLDQKLVDTLSDSDRIYIVACGTSYHAGLAGKQTLEKLTQIPVEVHLASEFGYNTPLL
                     SKKPFFIFLSQSGETADSRQVLVKINRLGYPSLTITNVAGSTLSREASFTLLLHAGPE
                     IAVASTKAYTAQIAVLTLLAKAIGDKKEFETSLVFDITHELSLVANAMESVIAQKDYL
                     EELAEVYLSDTRNAFYIGRGADYNVSLEAALKLKEISYIQAEGFAAGELKHGTIALIE
                     EGTPVIGIISEEVTGAHTRGNLKEVESRGAKTLVIVSEGLEKESDQLVLPTVHPYLTT
                     LTTVVPTQLLAYYATLLRGFDVDKPRNLAKSVTVE"
     gene            complement(14670..16025)
                     /gene="glmM"
                     /locus_tag="ACG842_001550"
     CDS             complement(14670..16025)
                     /gene="glmM"
                     /locus_tag="ACG842_001550"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010739004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HFQ0248097.1"
                     /translation="MGKYFGTDGVRGEANKELTPELAFKLGRCGGYVLSQHEEEGKRP
                     RVLVGRDTRISGQMLEEALISGLLSVGIEVFQLGVISTPGVAYLTRLQKASAGVMISA
                     SHNPAQDNGIKFFGADGFKLVDDQELEIEALLDAEVDELPRPSAEGLGTVEEFPEGLL
                     KYSQFLVQTINGDLSGLTICVDAANGATSTSVNRLFADLETDFYTMGTNPDGLNINDG
                     VGSTHPERLAEMVVEKGADAGLAFDGDGDRIIAVDELGNIVDGDKIMFICAKYLAEKN
                     RLKKDTIVTTVMSNLGFHKAVEGIGLKDVVTQVGDRYVVEEMRKNDYNFGGEQSGHMI
                     FLDFNTTGDGMLSGIQLLNIMKQTGKKLSELAAEVTIYPQKLVNIRVSDKNGAMDVPA
                     IKKVIEEAEAEMGSEGRILVRPSGTEPLLRVMAEAPTDEKVNYYVDKIAAVVKEEIGL
                     D"
     gene            complement(16049..17218)
                     /locus_tag="ACG842_001551"
     CDS             complement(16049..17218)
                     /locus_tag="ACG842_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CdaR family protein"
                     /protein_id="HFQ0248098.1"
                     /translation="MISKEKRTSILYSFIALLFSIALYFNANGQSVPNTLSGNEAYNQ
                     TVTGVPIKISYDSKRYYIHGYENAVTVKLSSANRVQLNAEANEDTRMFRVTADITKLG
                     EGTHEVPLKIQNLSSAVTAKIEPSTITVTVEKKVIKDFDVETLIPSTFTPSGYELDDT
                     SVSPKKVSITTGDKTLAEIKRVVADVDASMVTDDGINSEVPIQALNAAGEVLSIVSDP
                     VQVTVRADSIKPSKSVRLYGIQQGTPAAGVESYDFSFSKLEAEVSGSSDLLASIGDSI
                     AVPINVSGINHRTTRKIEIPVEEGLSIKPESVSVEITPVLQTSSSSSASTSSRTQENT
                     STSRSISSADSGTEPSTTTISSSSESGTSSSTTSTEETSETSESTQMSRTNSSEN"
     gene            complement(17215..18102)
                     /gene="cdaA"
                     /locus_tag="ACG842_001552"
     CDS             complement(17215..18102)
                     /gene="cdaA"
                     /locus_tag="ACG842_001552"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0006171 - cAMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase CdaA"
                     /protein_id="HFQ0248099.1"
                     /translation="MSFQIGELFNLDYWQQMFSGDLFSLNFLVNILDILVVWYLVYKL
                     IQLLRGTKAIQLLKGVAIFIVIRIVAEIIGLHTLSWLMNQVITYGVIAAIVIFQPEIR
                     RGLEHLGRSSLFKQSKSEQQEDEKMILSFDKAIQYMSKRKIGALITIERHTGLDEYIE
                     TGIALDADITGELLINIFIPNTPLHDGAVIVKEGKIAVASAYLPLSESMLIPKEFGTR
                     HRAAVGISEVSDAITIVVSEETGDVSITLDNELMAGLSQQEYLAILRRELIAEEKTKN
                     KKNVIQSFLDGMTKGGDRK"
     gene            complement(18291..21983)
                     /gene="nifJ"
                     /locus_tag="ACG842_001553"
     CDS             complement(18291..21983)
                     /gene="nifJ"
                     /locus_tag="ACG842_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HFQ0248100.1"
                     /translation="MRKMKTMDGNTAAAYISYAFTEIAAIYPITPSSTMAELVDQWSA
                     AGKENVFGQPVKVVEMQSEAGAAGVVHGSLKTGALTTTYTASQGLLLMIPNMYKIAGE
                     LLPSVFHVASRAVTTNALNIFGDHGDVMAARQTGFAMLAESSVQEVMDLAPVAHLASI
                     EGEVPFLNFFDGFRTSHEIQKIEVLEYEELAPLINQEKLASFRKRGMNPNHPTVSGTN
                     QNPDIHFQQRETINPYYDKLPEIVQKYMKEINRLRGTSYDLVTYYGADDAEEVIVSMG
                     SVAQTIEQTIDYLNANGRKTGFLNIHLYRPFPVETFVQKLPHTVKTIAVLDRSKEPGA
                     GGEPLLLDVQSALYDSELRPAVIGGRYGLGSKDVTPDQISAVFDELKKDPSIRKKRFT
                     IGIVDDVTYQSLEPKESLDLTEPQTFQAKFWGFGSDGTVGANKSAIKIIGDHTDKYAQ
                     GYFYYDSKKSGGLTVSHLRFGDTPIRSAYLVEHADLVACHTPAYLHSYDLVKGLKPGG
                     IFLLNTLWSDEQLETHLPLKLKRYLAENNIRFYTINAMRLAQEVGLGRRINTAMETAF
                     FKLADIIPFDEVLPLLKEEALKSYGHKSMAIVEKNIQAIDRTTELLHQVAIPDHWRTL
                     EMPEKINKRNTGYIQEILEPVNAQEGNQLSVGTLVANGMTTGEMPLGTASMEKRGVAL
                     EVPEWISDRCTMCNECAFVCPHAAIRPFLADEEEMEEAPEGFIVREMRGADGVKYRIQ
                     VSVEDCTGCGLCVEACPAKEKALVMKPYEEQKEQAVNWAFAMTLRQKANPVKGKNTVL
                     STQFNQPLLEFSGACSGCGETPYVKLLTQMFGDRMMIANATGCSSIWGGASPASPYTT
                     NECGQGPAWSNSLLEDNAEFGYGMLLAAQTRREALASSIKEVLPKVSEELRELMEDWL
                     VHMFESEGTQQRAAKLQAAIESERSIFPELEDIYKRKDLFVKNSQWMIGGDGWAYDIG
                     FGGIDHVLASGADVNILVLDNEVYSNTGGQTSKATPASAIAKFSASGKYASKKDLGMM
                     AMTYGNVYVAQIASGANQMQTIKAFEEAEKYPGPSLIIAYTPCITHGLLGGMKESLKE
                     AKEAVSSGYWSLYRYNPTMVEKNKNPMTLDYKKPDFSAMPDFMRKQVRFSSLDNANPN
                     LAKALFEKTVHDAKTRFYNYAALTGELEKIKKRLEPEESIDVPVKTRKEKTTDLEAEE
                     RRAARRAARAERRKKMEQATKEKKEK"
     gene            22182..22703
                     /locus_tag="ACG842_001554"
     CDS             22182..22703
                     /locus_tag="ACG842_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HFQ0248101.1"
                     /translation="MDISVIDATKTNTQKGILYGSSNAPKKMVEFINLACPYCRQWFE
                     ESYELLEEAVQSGQLQRVIKLFDKEKESLQRGNVMHRYLTISDGQKAIKEIKQIFDTQ
                     DEWKHLSLQEVADFAVDKLKLTEQKDEQLSQAVINEAEQAHIRFVPTVILGKEIFDES
                     ISIEELKELIQTK"
BASE COUNT         6326 a         4932 c         4041 g         7420 t
ORIGIN      
        1 ttttttattt ctatgcttct gctactgcag gagcttttct caataaacca taaatcacac
       61 cagatacgac tgctccgata gcaataaaca ccaaatacat gaccgggtga ctcagtaaga
      121 atacaacgaa aatcccgccg tgaggagcca gtaatttgat tccgaaagct ccgactaatc
      181 cgccagttaa agcagaacca ataataaaac ttggaatggc acgaatcggg tcagctgctg
      241 caaatggaat cgatccttcc gtgacaaacg atagtcccat cacgatgttc gtcaaaccag
      301 catcttgatc tgttttgctg aatttattct taaacaaacg tgtagcaacg aaaattgcca
      361 atggaggtac cattcctcca gccataacag aagccatcac gacacttccg ccagttgcaa
      421 cagtcgtagc taaagttgct gtaccaaaca cataagcggc tttgttgatt ggaccgccta
      481 aatctgccgc catcattcca gctaataatg caccaagtaa agcagcattc gaaccactca
      541 tacttgtcaa gaaaccgttc aatgcatcat tgattgcttt catagggaca ttgaccagca
      601 acatcaagaa accagtaatc agtaagccaa acactggata aaagaggatc gttttaattc
      661 catccaatga ttttggtaag ttcttgaata atttacgtaa aaatacgatg acataaccag
      721 ctaagaatcc accaatcagt gcacctaaga atcctgcacc gcctgtatta gctaatgcac
      781 cagcagcaaa tccaacgatc aaaccagggc ggtcaccgat acttgaagca ataaatcctg
      841 ctaataccgg cagcatgaaa ccaaatgcag cttgtccgat ttggttgaac cagctagcag
      901 cttgattgta attccctaga tttgataatt gatcatgtgg cacgccaata aattggtcga
      961 tcatgaaaga aagcgcaata gcgattcctc caccaataac gaaaggtaac atgtgtgata
     1021 cgccgttcat caaatctttg taaattcttt gtccgatagt accatccgca tttccttctt
     1081 ctgaactgct ttcttttcca tcaccatgga atactggtgc agtaccacta agggctagat
     1141 tgatcaactc ttctggttta cggataccat cactgacagg acgattgatc agttcttttc
     1201 cgtcaaagcg attcatctct acttttttgt ctgctgcaac gattacacct tctgcacggg
     1261 cgatgtcttc agcagtcaat cggtttttga ttccttctga accgttcgtt tcgactttga
     1321 tctccacacc catttctttc gcttttttct tcaaagcatc ttctgccatg taagtgtgag
     1381 cgatccctgt tggacaagct gtaacggcaa caacgaattt tctttctgga tgagcagaag
     1441 cttctgcagc ttgtgtttct tgttctgctt tttcttctgc ttctttttgc tcttcagctg
     1501 tttggaacaa gtcttggact tcttctggtg tatttgcttc ttttaatttt cctacgaaat
     1561 ctgcatcaat cagcaatcga gatagtgctg ctaatgcttg caaatgtgtg tcgtttgcgc
     1621 cttctggagc tgcgatcatg aaaaacagat aagttggctg tccgtctaag gcttcataat
     1681 ctactccttt ttggcttttt gcgaataaaa ctgttgcttc tttaactgca ctgtttttcg
     1741 cgtgaggcat ggcgattcca tcaccaagac ctgtagaagt ttgtgcttcg cgggctagaa
     1801 ttccttcttt atatgtttcg atatcggaaa tccggccggc atcaaacatt ttttggacca
     1861 tttcatcgat tgcgcctttt ttatcagtcg cttgcaaatc catgatcatc gcttctttga
     1921 ccattaaatc cttgatattc atgattatcc tccttgaaac taatcaattt ttgtgatttc
     1981 aacttctggc agcaactcat caatcaattc gcgagctgcc aagtcatcag aaaaagctgt
     2041 tgcgcttccg caagcaactc cccatttaaa ggcttcgatt ggatctttcg tacggctgta
     2101 agccccaata aagccagcaa tcattgaatc ccctgcacct actgaatttt tcaatgggcg
     2161 aactaataca ttcgttttat aagttccttg ttccgtgaag agcaaagcac cgtctccagc
     2221 cattgagatc agcacattct gtgccccttc agctagtaat tttttcccat acggaatgat
     2281 ttcttctaca gattggaagg ttgtgttgta aagctcagct aattcgtgat tgtttggttt
     2341 gatcaacaac ggtttcttaa caagtgcatt ttttaggtca tcacctgttg tatcaatcac
     2401 gaaatctgca cctttttctc gaatgatatc aatcagttct tgataaaatc cttttcgcag
     2461 gctagctggc gcgcttcctg acaatacgac gatatctcct tgtcctactg ttcctaatgc
     2521 ttgcttcaac tcatcgattt cttcatctga aatagatggg cctaagccat tgatctcagt
     2581 ttcagtgtct gattttagtt tgatattgat tcttgtatct tcttttacgg ttgtgaacaa
     2641 agactggatt tgttcttttt caagccactt cgaaataaat tcgccagtga accctcctaa
     2701 aaaccctagt gctgtagatt gagaatcgat ccgtttcaaa atacgggaaa cattgatccc
     2761 ttttcctcca ggtaacttga agtcttcctt cattcggttc aaatcgccta gttttaaccc
     2821 atctacgcgt acgatataat caattgatgg attaagtgtt actgtgtaaa tcattgtatt
     2881 gcctccttaa ttgttgtctt ttcacggaat tcattaacga attctaatgg acaacgctcg
     2941 gttatgatcg tggctgcttc caaaggtgca acagaggtga aggacaactg gttgaatttg
     3001 gtatgatcaa tcaatacaaa tgtctcttcg gaatttttca tggctaaacg tttaacagct
     3061 gcctcttctg gatccggagt cgtgaatcct tgttcaagat gggcaccgtt cattcccata
     3121 aatgctttat taaaatgaaa attttttagt tgttctatcg cattagatcc taaaacagca
     3181 ttagttgcca gctttatcgt tccacctaaa ataatggtag caagctccaa atcaactaaa
     3241 tttgccgcat gttgaacgga atttgtcact actgtgatct gttttccttg cagaaaagga
     3301 atcatttcta acgtagtaga gccagcgtct aaataaatga catcttcgtc attaatggtt
     3361 tcagctgccg atttggcgat ttgctgtttt tcgtgaacgt ttttgagtga tttttcactc
     3421 atattctgct caaaattcaa taattgctct ttcttagctc cgccatgaac tctttctaga
     3481 aacccactat tctccagctc ttgtaaatca cgtcttatcg ttgattcaga agcattcaaa
     3541 aggtttacaa gatcttgtga tttcacgatt ttctgctgtt caagcaagtc taggattttt
     3601 tgtctgcgtt cctctgtaag cattttcacg atcacctcaa tacaaataat agcatatttt
     3661 ttttctacta gcaatataaa tcagtcaaaa acgttcataa aaattataaa tcagtcaaaa
     3721 aaaaataaaa aaccagtcag aaagactggt tttttatttt tttgtctatc atcaagccgt
     3781 tttatcgttt tttattttcg cttcttgttt ttttcatata aacagaagca ggttttcttt
     3841 ccttatgaag aagataataa taaagtccca tcccgccaaa catgaccaaa aaaccgatga
     3901 ctgatcgaat aggaaaatta ggaacaaaaa agagacagac ggataataat gatgccggaa
     3961 ccattattcg ataatagagt tgtttatcca ttttcttcac ctcatatttt ttgtaaagta
     4021 atcaattgtt ctaattctcg gtaatctaaa aattgcacac gcagatgctg ctcacctttc
     4081 ttgacatcga attggatact tcgccgcttt ttctttattc gtttgaaggt aacatcagag
     4141 agtctatgtt tgatttcctt taacggtaat tctgacaggg attctgaagg aacatagatt
     4201 cttaaggata aaagatactc ttgatatttt tcatctgtta cctctacttc tagatattcc
     4261 ctaatataat aacaataagc ttcgattcca ttatccaaag caacagtcgg atcgataact
     4321 ttatagcaca tcatgcgtga tcgattctcg tcaacccaag gaaaatacgc ctgagcattt
     4381 agtgttccgc gaacacgcct ttctttataa tctccttgtt ggatatcata actttttgtt
     4441 acaaaaaatg aagcattctt tttaggaaca ggttgcattt gtgcattctg atactggaca
     4501 gattggatcg tatggtcagt gtaaatgtat tcaacgaatg gcggtttcaa taaaccgaca
     4561 ccattgtccc aagcaagatg atcaaaccca agctggtccc taaaatagga ttgaaacatt
     4621 tgattgtttg gcttttgcca gttaaagggg tctcgataaa gctggataca tatgacttcg
     4681 ttttctaatt ccttcacttt ttctacttgt tggatgtcga gaaaaatcgg tttagggatg
     4741 atttgatgat aatagcgact ataatacatt tcccaacacg aagtgaaaca taaaccttgg
     4801 taaaaaagat cgtatcctgg aaattggttg caatcgacta ctacttcccc gttttgttgt
     4861 ttcattttag gtaaatgttc gatttcatcc tttttctcaa acaatagccg ttgagagatc
     4921 tgttttgtat tgtgatagat aaactcgcta taagctcgca tataggcgaa caccccatga
     4981 gcaatcattt ttttctgaat gaagtctcga atcaaaaatt gcttttcttt gaaaacagag
     5041 atagacaata cattttttat caataattga ttatcttcca agcgaaaact acattcgttc
     5101 tcgtcatttc ctacaactaa ccaactttcg tctcttaccg atgctagcat tattttttct
     5161 tgaagctttc gaagatcata cggatccgct tgttgtgtga aagagcggat aaatgttggc
     5221 tggatcaaca gacgtgtcaa aatagcccaa tcgcttattt cctgtttttt ttcttcttca
     5281 tttaagtctg tgagctggat agtcatcaat aatttatctt cgccaatcat tttctcacct
     5341 ctttcaaatc tttttataat ttaattataa ccaatgataa agaaaaacta aaactttatg
     5401 tcttagaaac caatttggac gattacatga tagactaagt aaaaagaaac aaagaggtgt
     5461 ttacaatggg gttgaaattt gaacgtacag ataatttaga taaacttttc gaatcatttg
     5521 ctgtcgatcc ggaaaaaaca gttgcaccaa aagaagagat cgatcgattt ctaaaaccac
     5581 agccacaaaa tgaaaagaaa aaagacaaga aacaaaagta agttaagctc aaaaaaagac
     5641 ttaccagcca aacttttatg aaagagtttg actggtaagt cttttttatt ctttttatga
     5701 ttcaattgct tgttcgattg ctgcccacgc ttcttctttt ccctgttttg taacggaaga
     5761 aaagagaata aacgtgtcat tgcgatcaaa atccaacgct tttttgattt cgctctcatg
     5821 cttgttccat ttaccacgag ggatcttatc tgccttagtg gcaacaacaa ttacagggat
     5881 atcataatac ttcaaaaatt gatacatctg gatatcttct tgagaaggct tatgacgcaa
     5941 atctactaaa gaaacgaccg cacgtagctg gtctctagtc gttaaatatg tctcgatcat
     6001 ctttccccat tttgctcgct cagttttgga tacttttgca tacccatatc ccggaacatc
     6061 gacaaaatgt aatgcgttct cgatcaaata aaaattcaat gtctgggtct ttcctggctt
     6121 accggaagta cgggctaaat tctttcgatc aatcaatgtg ttgataaaag aagatttccc
     6181 tacatttgat cgtcctgcca gagctatttc tggcaattgt gtatctggat attgttttgg
     6241 tgccactgca cttatgataa tttcagcttg atggactttc atggtctctc tccttaacaa
     6301 ggtgatgaat aaagcaagcg gctgtcgcaa aagccaaaat gacttttacg gcagcctctt
     6361 ttcatcaacc cgcttttttc tctgaatctt tatagattac tgttggctca ccagacaatt
     6421 cagctgcttc ttttgtgata atcacttttt cgatgttttc atttgaaggg atatcaaaca
     6481 tcacatccat catgatttct tcaatgatgg aacgaagtcc tcgagcaccg gtattgcgtt
     6541 cgattgcttt tttcgcaatg gctctcaacg cttcaggttc gaattcaagt tcggtattat
     6601 ctagagaaag aagtttttga tattgtttga ctagcgcatt tttcggctca gtcaagatcc
     6661 gtaccagatc atctgttgtc aatttctcta aagctgccat aacaggtaat cgtccgataa
     6721 actcagggat caatccgaat tttagtaaat cttcaggaat gatatgctgc atgacacttt
     6781 cgtcttcaga aagtttttgg ttttgtgtac caaatccaat cgtcttttcg cccatacgat
     6841 tcttcacaat cgtttcgatg ccatcaaatg caccacctac gatgaacaat atgttcgtcg
     6901 tatcaatctg aataaattct tgatgtggat gtttacgtcc gccctgaggt ggcacacttg
     6961 caacagttcc ttctagaatt ttcagcaagg cttgttggac accttcgccg gaaacgtcac
     7021 gagtaataga tacattttcg cttttacgcg caattttatc gatctcatca atatagatga
     7081 tccctttttc tgcaagttcc acattgtaat cagcagattg aagcaattta agcaaaatgt
     7141 tttctacatc ttcccccaca taaccagctt ctgtcaaact tgtcgcatct gcgattgcaa
     7201 aaggtacatt caatgttttt gctaatgttt gtgctaaaaa tgttttacca gaccctgtag
     7261 gtccgatcaa gcagatattg cttttttgca gttcaacatc atctgtgttt tcttgtgctt
     7321 gctgattgac acgtttatag tgattgtata cggcaacagc taacgagcgt tttgcgcgat
     7381 cttgtccaat cacgtagtta ttcaatactt caagaatttc tagtggttta gggacttcag
     7441 tgaattcgcg tacagcttct tcataaaatt cttcatcaat gatttcttta cataggtcga
     7501 tacattcatt acagatgtag acccctggac cagctacgat ctttttcact tcttcttggg
     7561 ttttcccgca aaaagaacag cgtacggtct cattgctact tggattgtca tacatggtct
     7621 tcacccctta acttgcaaaa agtatgcaag aaactatcaa agactttaca gtcttttact
     7681 atcataacat agtttagtaa aaagaaaaag tgtttaacaa aggatcaact gacctatacc
     7741 tttgcatttt ttactcttaa caaaaaataa ggaactgtga caaaatcgtg tcacagtccc
     7801 ttatttcgaa taaacaattt tctagaagta gcttcttcag aaatgattta ttacttgatt
     7861 tctattcttt cgcttctgtc gctgtacctg tgatcaattc gattgctttt ttcattgtca
     7921 catcatgttc cagcatatct tctgtcaaca cgcgtttgat ttgttcgatt ggcatgttgt
     7981 atgtttctga taattcttgg atttctgcat tgatttcttc ttctgttgct tgaagatttt
     8041 cagcagctgc aattgcttcg atcacaagat ttgttcttgt gcgcatttct gcttcacctt
     8101 cgaattgttt atgcaagtct tcttctgtag aaccagttaa ttgatagtac atttcaggag
     8161 agataccttg acgttgcatg ttgttcaaga attcgtccat tgaacgatgt acttcatcat
     8221 gaaccatcac gtgtggcaat tcaacgattt ctgcgttttc aacagccata cggatcgctg
     8281 cttcatcttt tgcatcgtcc gctgcttttt cttttgtttc tactaattct ttgcgatatt
     8341 tttctttcaa ttcgtctaat gttgatactt cgtcatcaac atcttttgcg aattcgtcat
     8401 ctaattcagg cacttctttt gcttttactt catggattgt cactttgaag actgcttctt
     8461 ttccagcaag atcttctgct tgataatctt ctgggaaagt tacatttaca tctagacttt
     8521 cacctgattt atgaccaact aattgatctt caaaacctgg aatgaatgag cctgaaccta
     8581 attcaagtga gtaattttcg ccttttccgc catcaaacgc ttcgccatct acaaagcctt
     8641 cgaagtcgat tactactgta tcgccatttt cagctgcagc atcttctttg ataactaatt
     8701 cagcttgtgc ttcttgttca cgtttgatac gtgcatccac gtcagcatct gttacttcac
     8761 ggtcttgttt ttcaacaact agatctttat attgtcctaa ttttacttct ggtttcactg
     8821 ttacttcagc agtgattacc cagtcttgac ctttttccat gctttcaaca tcgatttttg
     8881 gttgagaaac aggatcgatg ccagcttctt tgacagcagc ttcataagct tctggtaata
     8941 cagcattcaa tgcatcttca tataaagctt cttcaccgta catacggttg aatacttgac
     9001 gagaaacttt ccctttacgg aatccaggta cgttcaagct ttttttcact ttattaaacg
     9061 cagttgttaa tcctttttgg atttcgtctt gcgcaatcgt aaattttaaa acaccatcat
     9121 tcgtgccttt tttttcaaat gttgcagtca tttaattccc tccgagttta gaaattcatt
     9181 ttatacatca aaaacgtttc atgcatacct ttaaatagta cactaaccct ttcaaattgt
     9241 aaacatattg aatggataga atccttgttt ttcataacat ttttatcttt ttcactgata
     9301 aaatctaatg gaagaagatc atttgctgca aaattttttc tctttttttt aaaaaatctt
     9361 gtttttcatc ttctgtacct tcatacatcg tttttcctgt acacgcctct acagtcagtt
     9421 gaaaccagcg cttcgggtct tcgatgtatg tatcatagac aggataaaca attgcagatt
     9481 gcaacgtgaa ttcttctttt aacacggaac taagcagcgc atcttcattc ccgtaacgat
     9541 ctgaaaatac acataattgc tggtaacttg cctgctgctg cggctttggc agatcttttg
     9601 gtatgacttt gactaacttt tcttcaatcg tcaaatagaa aacttgatgg cattcgttca
     9661 attgcctaag tgtctccagt aattttgccc ttgctaaaat atggacctct ggcgatacaa
     9721 taagtttact cgctgtttgg ataaactcat gataaggaag ctgttcgatc tcatcaatag
     9781 acgcaagctg acgtgcaggt tccaacgaag gaagttcctc cgatttattg tggagtgcct
     9841 tgatcttctg cctctccatt tgtccggata attcttctgc aaattggatt ttttcaatga
     9901 cccgctgctc gaaagcttca taaaaatctg tacttgctat caattttcta gcatataaaa
     9961 aatcttgtgt actgattaac aagtcaaaat aaaattcagc aaactcctca ttttgaagat
    10021 actgtttttc atgcaagagt gcttgcttca atgcttcttt tttctcgtcc aactcgtaaa
    10081 gacaaaaaac aagcaggcta ttagcctcaa agctttcttt caactcgtaa gctcgttgaa
    10141 ataattcttt tgcctgctga tattgctggt ttgctagtgc ttgttgtgcc actcgcatta
    10201 aattctcata tttttctgga aaatcaatcg gcttcacgca gtgtcctcct cttgctcata
    10261 ttcagctcat gttcaatagt agcaaaacaa aaaaagcatg acaattaaaa ataactgaag
    10321 caccctctaa aagctagatt ttagatctga cttttagaag gtgctttcaa ctgttactgc
    10381 tttttattgc tcatcagctg ttgacttcct ggttgaacgc tttgatcaga ttgatcgtaa
    10441 caacacttgt attagtgaaa aaatcgccca gatgttgctt tttctgatta aagactgctg
    10501 gaagataccc taacatcagc aaaggaaatt ttagaatgaa ccggcaagca ccttctctaa
    10561 ctaaaacggt tgaccagcta agttcttctt catcaaagct gatgactcga ataccaaaga
    10621 tcattttccc aatcgtttgg ccatgattga atttcgtcaa taaaataaaa tacgctagat
    10681 aaataagtaa ggctgtgaat ccgtagagac tcagataaga atctgaaaaa ttaagccaac
    10741 ctagattttt ggcaatacca agtgttaaac gcgtaatagc tccgatacac aaaagatcga
    10801 ccaagaaggc aaacattcgg ataaaaaaac ctgcatagaa atacttagga aaatcatttg
    10861 cttttggcgg ttcatttctt gtgtccgtat actttttcca tttacgttgt ttctttttga
    10921 tttcttcggg cgtcaaaggc tctctttcaa aagagctcag taccttttgg gaaaagttgg
    10981 agtcaggtcc tacagcaggc tgctgttgct tatctttttc ttgttcctta ttttcatttg
    11041 aaagctctga catgcttact cacctccgta ataatacatt gcttttggtg attccgctgt
    11101 ccctagattc tcaagaacgg ataagatctg actagtctca gaaggcttca atccctggaa
    11161 ttctgctagt ttacttccta accatgtact agcaaaccca gatggcgtgg aagaatattc
    11221 tactagttcc gcatcttcaa gctgttgttc ctttcgtata gcttttaaag catcttcagg
    11281 aaatcccaat tcatcgacta aacctacttc ttttgcttgg acaccatcat aaatccttcc
    11341 atcagcaatt tttttgactt cttcttcaga tttattccgt ccctcgctga cgattgctac
    11401 aaaacgacta tatgcgctgt cgatatatgc ttgaagcacc gcttcttctt cttttgtctc
    11461 tggtcgcgtt cctgaaccca tatctttcaa cgctccgctt ttaactgttg tatcttccac
    11521 acccagtttt tctagaagtc ctgaataatt caatcctgac atgatcacac caattgagcc
    11581 ggtcactgtt tcttctgttg caaagatttt atctgcttga gcagaaatat agtagccacc
    11641 gctagctgcc atgtttttca tgctgacata cattgggata tcatgttctt tacgaatcgt
    11701 atccaatttc ttagcgattt ccgcactttc gtagacaccg ccaccaggag aatttacttc
    11761 taaaaagata cctttgactg ttggatcttc ttcaatcgcc ttcagttgtt ccataaaatt
    11821 tttatgattg taaccttcag aagcaaataa acctgaagac cctcccgcag caatcgttcc
    11881 atctactgat aattttacga ttttttgcgt actgtctccc tcttccaaca catttggagc
    11941 tagttcttca tccccatata accaactgtt caatccctca atttgatcgt tttcctgagg
    12001 cttagtcaaa ctagctgaaa ctaacgaaac cacaaataat cctgcagcaa tcagaactgc
    12061 tgcccaacgt cttttattca ttctttctcc tttctaatac aagtttttta tgacgatcac
    12121 agtttctgac tgctcagaag tgtcgaaccg ctctgctttt tcttcccgcg catacccgga
    12181 aaacgactct tttgttaatt catcttctac cgtaaactca cgagcttgag caacttccaa
    12241 ggcatcaggt gcgatttctt ggatgctatc ccagccagta tctaaaaata ttttcgtatc
    12301 atctgataca ccagctgatg cttcgaattt ggacaattgt ttttttaaca ttcccagtgt
    12361 catgatctca atttttgaat ccatttttac tcctcctcta tccgattact ttctattgta
    12421 ccgaactcct gaaaaagatg atagataaaa acccattatt ttttgacata aaaaggacat
    12481 ttgacaaaga atttgatata taccaattta ttttttattc tttttttgtt gacaagaaca
    12541 ttcgcaaaag gttatgattt taaatgtttc cagataaaaa acaaaaagga ggatagttat
    12601 accttttagg atagcgccag gtctgtgtaa acagatgacg aggagagggt ttatcgaaag
    12661 attcggcgga tggccctagg gactgcactc tacagaaaat ccacaaaaaa tgtctgcaaa
    12721 gataaaacaa aaggattttc ctgcagctgg aagcattcat acgaattcat tatagaaagt
    12781 aggatgtttt ttatgtgcgg aattgttgga atgattggat taaaaaatgt aacaccagga
    12841 ttgattgacg gtctagaaaa attagaatac cgtggttatg attcagctgg tatctttgtc
    12901 tcagatggca caactgatta tctggtcaaa gcacaaggca gaattcaaaa tttgaaaaac
    12961 aaaataaata ttgatactac aggaaatata ggtattggtc atacacgatg ggcaactcac
    13021 gggcaacctt ctgaagaaaa cgcccatcca catacttccc aaagtggacg cttcgtgctc
    13081 gttcacaacg gcgtaatcga aaactttgaa gaacttaaaa ttgcttattt agcaaatgac
    13141 cactttattg gtgagaccga tactgagatt atcgctcatc taattgaaac tttcgcgaag
    13201 gatactacaa cgcaagaggc gtttttaaaa gctttgcggg tcatcaaagg ttcttacgct
    13261 ttcgctttga ttgatcggac agcacctgat gttatttacg tcgctaaaaa taaaagtcca
    13321 ctattaatcg gactcggcga tggattcaat gtgattgcca gcgatgcaat ggctatgctt
    13381 gctcatacga aggaattcgt tgagattgaa gatgaagaga tggttaccgt cacatcagaa
    13441 aaagttatta ttcaaaattt tgcaggtgac atcgtagaac gttcttcatt cgaagcacaa
    13501 gtagatgctt cagatatcga gaaaggtacc tatccttttt atatgctgaa agaaatcgac
    13561 gaacagccta ttatcatgcg tcgtatcgct caaaaatacg taacagaaga caatcgtgtc
    13621 tctcttgatc aaaagctggt cgatacattg tctgatagcg atcgaatcta tatcgttgct
    13681 tgcggaacta gttaccatgc ggggttagca ggtaaacaga ctttggaaaa attgacacag
    13741 attcctgttg aggttcattt agccagtgaa tttggctata acacacctct tctttcaaaa
    13801 aaaccattct ttattttctt gagtcaaagt ggcgaaacag cagacagccg ccaagtgctg
    13861 gtaaaaatca accgtctcgg ctacccttca cttacgatta caaatgttgc tggttctact
    13921 ctttcgcggg aagcatcctt tacactgcta ttgcatgctg gacccgaaat cgcagtagca
    13981 tcaacgaaag cttatactgc acaaatagct gtactaactt tattagccaa agctatcggt
    14041 gataaaaaag aatttgaaac ttctcttgta ttcgatatca ctcatgaatt gagtttagtt
    14101 gctaatgcga tggaatcggt catcgcccaa aaagattatt tagaagagtt ggcagaagta
    14161 tatctcagcg atacaagaaa tgccttttac attggacgtg gtgctgacta caacgtttct
    14221 cttgaagctg ctttgaaatt gaaagaaatc agttatatcc aggcagaagg ttttgccgct
    14281 ggcgaattga aacatggaac gattgctttg atcgaagaag gaacaccagt gatcggaatc
    14341 atcagtgaag aagtgactgg tgcgcatact cgaggaaacc tgaaagaagt cgaaagccgt
    14401 ggcgctaaaa cactagttat tgtctcagag ggattagaaa aggaaagtga ccagctggtg
    14461 ctaccaactg tccaccctta tctaactact ctaaccacag tcgtaccgac tcaattactt
    14521 gcatattacg caacgctatt acggggattc gacgttgaca aaccacgtaa tttagcgaaa
    14581 tcagtgacag tagaataata aaagaacaag gaccagtgtc agacatctga cactggtcct
    14641 tgttctttta ttttttatga aaatatctct taatccagtc cgatttcttc tttgactact
    14701 gccgcgattt tatcgacata gtaattaact ttctcatcag taggcgcttc tgccatcact
    14761 cgaagtaatg gttctgtacc ggaaggacga actaggattc ttccttctga tcccatttca
    14821 gcttctgcct cttcaatcac ttttttgatt gccggaacat ccatcgcacc gtttttatca
    14881 cttacgcgga tattcactaa tttttgcgga tagattgtta cctctgctgc aagctctgat
    14941 aatttcttac ctgtctgttt catgatattc agtaactgaa ttcctgaaag catcccatct
    15001 ccagttgtgt taaaatctaa gaaaatcatg tgcccagact gctcgccgcc aaagttataa
    15061 tcgtttttgc gcatttcttc tacaacgtaa cgatcaccaa cttgtgtaac gacatctttc
    15121 aaaccgattc cttccaccgc tttgtggaat cctaagttac tcatgacagt cgtaacgatc
    15181 gtatcttttt tcaatcgatt tttttctgcc aagtatttag cacagataaa catgatttta
    15241 tcaccgtcga caatgtttcc aagttcatcc accgcaataa tacgatcgcc atcgccatcg
    15301 aaagctagtc cagcatctgc tcctttttca acgaccatct cagctaaacg ttctggatgg
    15361 gtagagccta cgccgtcatt gatatttaac ccgtctggat ttgtgcccat tgtataaaaa
    15421 tctgtttcaa gatcggcaaa caaacgatta acacttgttg aagttgctcc gtttgctgca
    15481 tcgacacaaa tggtcaaacc ggaaaggtca ccgttaatcg tttgtacaag gaattgagaa
    15541 tatttcaata agccttctgg aaattcttct acagttccca atccttcagc tgatggacga
    15601 ggcaattcat ccacttcagc atctaataat gcttcaattt ctagctcttg gtcatctact
    15661 agtttaaaac catctgcacc gaaaaatttg attccattat cttgtgctgg attatgagaa
    15721 gcagaaatca tgacccctgc gcttgctttt tgcaaacgtg tcagataagc aacacctggt
    15781 gtagagatca cacctagctg gaacacttcg atacccaccg ataaaagtcc tgaaatcagc
    15841 gcttcttcta acatttgacc tgagattctt gtatcccgcc ctactaacac gcgtgggcgt
    15901 tttccttctt cttcatgctg gcttaggaca taacctccgc accgtcccag cttaaatgca
    15961 agttctggcg taagttcctt gttggcttca ccgcgtacgc cgtctgttcc aaaatattta
    16021 cccatagttg taaaattact cctttaactt aattttctga ggaatttgtt ctgctcattt
    16081 gtgttgattc gcttgtttct gatgtttctt ctgtacttgt ggtcgagctg cttgtcccag
    16141 attccgaact ggaagaaatg gttgttgtcg atggttccgt tcctgaatca gcagatgaaa
    16201 tacttctact cgtacttgta ttttcttgtg tccgactgct cgtcgaagca gagctactgc
    16261 ttgacgtttg caacacgggt gtaatctcca cactaactga ttcgggcttg atacttaacc
    16321 cctcttctac tggtatttca attttacgag ttgttcgatg attgatacct gagacattga
    16381 tcggtacagc aatactgtca ccgatcgatg caagcaaatc gctagagccg cttacttctg
    16441 cttccagttt tgaaaaactg aaatcatagc tttctacccc ggcagctggt gttccttgct
    16501 gtatcccata aagtcgtacc gacttgcttg gcttgatcga atcagctctc actgttactt
    16561 gcactggatc agagacgata cttaatactt cccctgctgc attcagcgcc tgtattggga
    16621 cctcactatt aatcccatca tctgtgacca tcgaagcgtc cacatcagct actactcgtt
    16681 tgatttctgc caatgttttg tcacctgtag tgatagagac ttttttagga ctaacggaag
    16741 tatcatcaag ttcatatcca gatggtgtga acgttgaagg aatcaacgtt tccacatcga
    16801 aatcttttat tacctttttc tcaaccgtta ctgtaatagt cgatggttcg atctttgctg
    16861 tgacagcact gctcaaattt tgaattttta gcggaacttc atgtgttcct tcgcccagct
    16921 tagtgatatc tgctgtgacg cggaacatcc gtgtatcttc gtttgcctct gcattcaact
    16981 gaactcggtt tgcactggaa agtttgacag tcactgcatt ttcatatcca tggatatagt
    17041 agcgctttga atcataagaa atcttgatcg gcacccccgt aactgtttga ttatacgctt
    17101 catttccaga aagcgtattt ggaacggact ggccatttgc attgaagtac agcgcaatac
    17161 tgaacagcaa tgcaataaaa ctgtaaagaa tgcttgtccg tttctctttt gagatcattt
    17221 gcgatcccct cccttagtca taccgtctag gaaagattga atcacgtttt tcttgttttt
    17281 tgttttctct tcagcaatta gttctctacg caagattgcc aaatattctt gctgggataa
    17341 gccagccatc agttcattat ctaacgtgat gctgacgtcc ccggtttctt cagagacaac
    17401 gatggtaatc gcgtcactga cttcgcttat accgacagct gcccggtgtc gagtcccgaa
    17461 ttctttaggg atcaacatac tttctgatag aggaagatag gcgcttgcta ctgcgatctt
    17521 tccttctttt acaataactg caccgtcatg cagcggtgta ttaggaataa agatattgat
    17581 cagcagctcc ccagtgatat ctgcatctaa agcaatccct gtctcaatat actcatctaa
    17641 tccagtatga cgttcgatag tgatcaacgc accgattttt cgcttggaca tatattggat
    17701 ggctttgtca aaagacaaga tcatcttctc atcttcctgc tgttcgcttt ttgattgttt
    17761 gaaaagcgaa ctgcgtccaa gatgttccag tcctctccgg atttccggct gaaaaatcac
    17821 gatagcagct atcaccccgt acgtaatgac ttggttcatc agccaagaca aggtgtgcag
    17881 accaatgatt tccgctacga ttcggataac aataaaaata gctacacctt tcagaagctg
    17941 aatcgctttt gtgcctcgca gcagttggat cagtttgtat acaagatacc agacaaccag
    18001 aatatcaagt atgttcacca aaaaattcag agagaacaag tcaccggaaa acatttgctg
    18061 ccaatagtcc aaattaaaaa gttccccgat ttgaaatgac ataaaaagcc tctcttctct
    18121 tttgagattt cctctctagt atatcatatg ttttaatttt agaggctgat atgctcgtat
    18181 ttttcttgag aaaaacgcat aattttcaag aaaattgcaa gtatgcgttt tatttttctc
    18241 tgcaataaat atttttaaac ttcagagaat tattcattat attttttctg ttatttttcc
    18301 tttttttctt ttgtagcttg ttccattttt ttacgtcgtt ctgctctggc agctcttcta
    18361 gcagctcttc tttcttctgc ttcaaggtcc gtcgtttttt cttttctagt cttaacgggt
    18421 acatcgatag attcctctgg ttcaagtcgt ttcttgattt tttctaattc tcccgtcagt
    18481 gcagcatagt tgtaaaaacg tgttttagcg tcgtggacag tcttttcaaa taaagctttt
    18541 gcaagatttg gatttgcgtt atctagcgag gaaaagcgaa cttgtttcct catgaaatca
    18601 ggcatagcag agaaatcagg ttttttgtag tccaaagtca ttgggttctt gtttttttcg
    18661 accattgtag ggttatatcg atataatgac caataaccag aagaaacagc ttcttttgct
    18721 tctttaagcg attctttcat tccgccaagc aagccatgtg tgatacaagg cgtataggca
    18781 ataatcagag aaggaccagg atacttttct gcttcttcaa atgccttgat cgtttgcatt
    18841 tgattggcac ctgaagcaat ttgcgccaca taaacatttc catatgtcat cgccatcatt
    18901 cctagatctt tctttgaagc atactttcca ctagccgaaa atttagcgat tgccgaagct
    18961 ggtgttgctt ttgaggtttg accgccagta ttggaataga cttcgttatc caaaacaagg
    19021 atattcacgt ctgctccgct ggcaagtaca tgatcgatcc cgccaaaacc aatgtcgtaa
    19081 gcccatccgt ctccaccaat catccattgg ctgtttttta cgaatagatc ctttctcttg
    19141 tatatatcct ccagttcagg gaaaattgat cgttcgcttt cgatcgcagc ttgcagtttt
    19201 gctgctcttt gctgcgttcc ttcactctca aacatgtgta ctaaccagtc ttccatcaat
    19261 tctcgtagtt cttctgatac ttttggcaat acttctttga ttgaacttgc caaagcctcg
    19321 cgacgagtct gtgccgctag aagcatacca taaccaaatt cagcattatc ttccaataaa
    19381 gaatttgacc atgcaggccc ttgaccacat tcattggtcg tataaggaga agcaggagaa
    19441 gcaccgcccc aaatagaaga acagcctgtc gcattcgcga tcatcattcg atcaccgaac
    19501 atttgtgtca ataattttac ataaggtgtt tcgccacagc ctgagcaagc gcccgagaat
    19561 tcaagaagcg gttgattgaa ttgtgtagac aagacagtat tctttccttt gactggattt
    19621 gctttttgtc ttaatgtcat tgcaaaagcc caattcacag cttgttcttt ctgttcttca
    19681 taaggcttca tcactaaagc tttttctttt gcgggacaag cctctacaca cagaccacaa
    19741 cctgtacagt cttccactga aacttggata cggtatttta cgccgtctgc gcctcgcatt
    19801 tctctcacga taaatccttc tggcgcttct tccatttcct cttcatctgc taaaaatgga
    19861 cggatggcag catgaggaca aacaaaggca cattcattac acatcgtaca gcgatcacta
    19921 atccactcag gtacttctaa agcgacgccc cgcttttcca tagaagccgt tcctaaaggc
    19981 atttcgccag ttgtcattcc atttgctaca agtgtaccaa cagagagttg atttccttcc
    20041 tgtgcattca ctggttcaag tatttcttgt atataaccag tgttcctttt attgattttt
    20101 tcaggcatct ccaacgttct ccaatggtct gggatcgcga cttgatgaag cagttcggtc
    20161 gtccgatcga ttgcttgtat gtttttttct acgatagcca ttgatttatg tccatagctt
    20221 ttcagggctt cttcttttag cagcggaagc acctcatcaa aaggaatgat gtcagctaat
    20281 ttaaaaaaag ccgtttccat tgccgtattg atccggcgtc ccaaaccgac ttcttgggcc
    20341 aatctcatgg cattgatcgt atagaatcta atgttatttt cagccaagta acgttttaat
    20401 tttaagggca gatgcgtttc taattgttcg tcgctccaaa gagtattcaa taaaaaaatg
    20461 ccacctggct tcaatccttt gaccaagtca tagctatgca ggtaagctgg tgtgtgacag
    20521 gcaacaaggt ccgcatgctc gacaagatac gctgaacgta tcggcgtatc tccaaaacga
    20581 agatgagaga cagtcagacc gccagatttt ttcgaatcat aataaaaata gccttgcgca
    20641 tatttatcgg tgtgatctcc aataatcttg atagctgatt tatttgctcc aactgttccg
    20701 tcagatccaa atccccaaaa cttggcctgg aatgtttgcg gttctgtcaa atccagactt
    20761 tcctttggtt ccaaagattg ataagtcaca tcatccacga tccctatggt aaaccgtttt
    20821 ttgcgtatgc ttgggtcttt tttcaattca tcaaaaactg ctgagatttg gtcgggagtc
    20881 acatctttag accccaatcc gtagcgacca ccaataacag ccgggcgaag ttcagaatca
    20941 tataacgcac tttgaacgtc taataagaga ggttctccac ctgcccctgg ttctttactt
    21001 cgatcaagaa cagcaattgt ttttactgta tgaggcaact tttgcacgaa tgtttccact
    21061 ggaaaaggac gataaagatg gatattcaaa aaacctgttt tccgtccgtt tgcattcaag
    21121 taatcaatcg tttgttcaat cgtctgtgca actgatccca tagaaacaat gacttcttcc
    21181 gcatcgtctg ctccgtaata agtcaccaag tcataagaag ttcctcgtaa tcggttgatc
    21241 tctttcatat atttttgaac gatttcaggt aatttatcat aataggggtt gatcgtttcc
    21301 cgttgttgaa aatggatgtc tggattctgg ttcgtaccac taacagtcgg atgattggga
    21361 ttcatccctc gtttacgaaa tgaagctaat ttctcctgat tgatcagagg agctaattct
    21421 tcatattcca aaacttcgat tttttgaatc tcgtgactcg tacggaatcc atcaaagaaa
    21481 ttcaaaaaag ggacttctcc ttcaatagaa gcaagatggg caacaggtgc taaatccatc
    21541 acttcttgta cactgctttc agctaacata gcaaatcctg tttgtcttgc agccatcacg
    21601 tctccgtgat ctccgaaaat attcaacgca tttgtcgtca ctgcccgact agcaacgtgg
    21661 aaaacacttg gcagtagttc cccagcaatc ttgtacatat tcggtatcat caaaagcagc
    21721 ccttgtgaag cggtatatgt agttgtcagt gcaccagttt tcaatgatcc atggaccaca
    21781 ccagctgctc ctgcttcgga ttgcatttct actactttta ctggttgacc gaatacgttt
    21841 tcttttcctg ctgctgacca ttgatctact aattcagcca tggtcgaact gggtgttatt
    21901 ggatatattg cagcaatctc ggtaaatgca taagaaatat atgcggctgc cgtattgcca
    21961 tccattgttt tcatttttcg catgattcat catccttgtt catattctct cttattatct
    22021 gttccattat aacgtaaaac atgccatttt tttcttttat ttataaaaat attttacatg
    22081 tatctatttg aattggtcat ctaattttta gacaaacagt ctaaatcgtt gccttttttt
    22141 gtcagacatg tataaactat aagcgaaaga ggtgttttaa catggatata tcagtgattg
    22201 atgcaacgaa aacaaataca caaaaaggaa tcctttatgg ttcatcgaat gctcctaaaa
    22261 aaatggtcga attcatcaac ttagcttgtc cgtattgccg acaatggttt gaagaatctt
    22321 atgagctatt agaagaagca gtccaaagcg ggcaattgca gcgagtgatc aaattgttcg
    22381 ataaagaaaa agaaagctta cagcgaggga acgtgatgca ccggtatctc acaatcagcg
    22441 atggccaaaa agcgatcaaa gagatcaaac aaatattcga tactcaagac gagtggaaac
    22501 atctttctct ccaagaagta gcggattttg cagtagataa actaaagctg acagaacaaa
    22561 aagacgaaca gctgagccaa gctgtcatca acgaagctga gcaagcacat attcgatttg
    22621 ttccaactgt catcttgggt aaagaaatct ttgatgaatc tatttcaata gaggaattaa
    22681 aagaattgat ccaaacaaaa taaaaaaata aggagcgtg
//
LOCUS       DAZKLM010000045        22422 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3764
            SAMEA115706913-rid22582563.denovo.045, whole genome shotgun
            sequence.
ACCESSION   DAZKLM010000045 DAZKLM010000000
VERSION     DAZKLM010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706913
            Sequence Read Archive: ERR13196647
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22422)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22422)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287823.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287823.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,004
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,849
            CDSs (with protein)               :: 2,849
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 41 of 96
            Pseudo Genes (incomplete)         :: 52 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 15 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22422
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706913-rid22582563.denovo.045"
                     /isolate="VRE3764"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            6..305
                     /locus_tag="ACG842_001555"
     CDS             6..305
                     /locus_tag="ACG842_001555"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFQ0248102.1"
                     /translation="MKNRLIVACGSGVATSQTIASKIQSMLEDDGIAFPVEAVDYKSI
                     QNELLTAGIYVYVAQPDEEVLEQAKDLGIEVFPGIPFLTGMGVEPIYDSIKELVQ"
     gene            378..1739
                     /locus_tag="ACG842_001556"
     CDS             378..1739
                     /locus_tag="ACG842_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIC"
                     /protein_id="HFQ0248103.1"
                     /translation="MEVFQTIINYVLNLGSAIFVPLIILILGLLAGMKLKKAFMSALT
                     LGIAFSGMSMVIGYMSNAVSPASEALAKNTGISLPALDLGWTGAASITWSWSYAFIFF
                     AVTIGVNFVLLLFNWTKTLNVDMWNVWGKALTAYLVYYVSGSLAAGFLTAMVQVILEL
                     KLGDMFQKHIQDLTGIPLVTVTHFMTSAAVLLLPFNMIMDKIPALNKRADTNALKKKI
                     GIFSENSVMGFIIGLGLGFAAAYGVSGSLNLAIQTATALSLFPMISKMFMQSLSPLAD
                     AMSEFMKKRFKDREVYIGLDWPILAGRSEIWVTAILLVPVFIGYAIILPGNQVLPLAG
                     IINYSIAVGGLLLTGGNLARMLILGIITMPIYLYGATYLAPILSDLAEKSGAVEGIKK
                     GQLITWSSIEGPEFRVLFAEAFNRNILAIVGCVIFLGLFVLLYKYMIKAKVPSERYAE
                     LQK"
     gene            1761..2021
                     /locus_tag="ACG842_001557"
     CDS             1761..2021
                     /locus_tag="ACG842_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0248104.1"
                     /translation="MKREETDKIKWTVALCGTLLLFLYGLFTQNIIINLLVIFFALVI
                     YKYGNHVLFREYDEKRKRKIEESMKIKEATKEILREKSFIKR"
     gene            2035..2787
                     /locus_tag="ACG842_001558"
     CDS             2035..2787
                     /locus_tag="ACG842_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HFQ0248105.1"
                     /translation="MSDGRMLFERERQDMARIVRVIFERKNTNVAGGNFSFKTTDDQG
                     KDYIIMTPTMMSEAYLGEISPAQVLVVEPRTRRIVDGVGHLTREINMHEAMYETNPAI
                     KAVLHAHAPNAMFWATTGLPMPNLTEATAKVKEIPVMDFEPNCSEELAELTSDYVKKQ
                     HLELPHMLLLNSHGVLINTGGESGIEAIHKALQILDTVEWNAEIAYKQTIFQALGITD
                     GFYSKGEKIGGLEELKHKTAIYNKSVIAAGGD"
     gene            complement(2881..3486)
                     /locus_tag="ACG842_001559"
     CDS             complement(2881..3486)
                     /locus_tag="ACG842_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFQ0248106.1"
                     /translation="MTYIFEKGTSGKHLILLHGTGGDEHSLLDIAHFLAPNSTLLSFR
                     GTVQEDGMNRFFKRNGLNQFDLDSLEKETDKLHEKIKEISEKELIPLSDWLLVGYSNG
                     ANIAAHLLLERETELNKGLFFHPMSLGVHQSRSTLEDKTVWISYGDNDPIVSPASFKE
                     LADQFETRQANVTIQLTVTGHQLTMDEIKQAREWTDHLLTK"
     gene            complement(3539..4513)
                     /locus_tag="ACG842_001560"
     CDS             complement(3539..4513)
                     /locus_tag="ACG842_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
                     /protein_id="HFQ0248107.1"
                     /translation="MDTQIRGIHHVTAMTSSATKIYRFFTDILGMRLIKKTVNQDDIE
                     TYHLYFTDDTGAPGTDMTFFDFPNQPKGKKGTDTISRASFRVPSDAALDFWLERFEKY
                     DVTHSAINERFGKKYIEFEDFDNQYYQLISDENNQGVAAGIPWKNSNVPTEFAITGLG
                     PVFVRVSQLEHIRLILEKILGFKETDHSGDFYLFEIGEGGNGASIIIEHRTDLPKAIE
                     GFGNIHHLALRVADKEALEYWIQVINKIELPNSGFVDRFYFQSEYFLAAHHVLFELAT
                     DGPGFLEDEPAESAGEKLSLPPFLEERREAIEAYVRPFDTSDANKKRE"
     gene            complement(4891..5610)
                     /locus_tag="ACG842_001561"
     CDS             complement(4891..5610)
                     /locus_tag="ACG842_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFQ0248108.1"
                     /translation="MPKYEEIANILRERIRSKEYPLDSFLPNQVDLVKEFGASRMTIK
                     KAINILAMEGLVYSQRGSGTKILNHPFMQKDTSPANEYEGLSLQMKRRHRSLKSQIIH
                     FDVEFPNQYIQERLMIDAEQPVYNIHRLRILDGEPYVLEHTFMPVHLVPGLKKEHLLA
                     SIYDHLHQELNVHFAGAYRAISADKSDEYDQQYLNCAENDPVLEVQQVVYLKDGQPIE
                     YSSSRNRFDVRNYSVLDVRST"
     gene            complement(5737..7008)
                     /gene="celB"
                     /locus_tag="ACG842_001562"
     CDS             complement(5737..7008)
                     /gene="celB"
                     /locus_tag="ACG842_001562"
                     /EC_number="2.7.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0019191 - cellobiose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HFQ0248109.1"
                     /translation="MNGFMDKLSEKIMPLANLLGQNRYLTVLRDAFMLSFPLTMFGSI
                     VVVINNLPFFSDATKGTLSNLFGNGQNATMSIMSVFVTFGIGYYLSKSYDVEGIFGGA
                     VSFASFLILTPFAMKTAGGEDVTGVLSLDRLGAKGMFIGMIAAFLAGEIYCRITKRGW
                     QIKMPDGVPPAVTKSFAALIPAVVTLTIFLVINAVMTGIFNANLHDVVYEVIQKPLTG
                     LGSSLPATLIALFFVQFLWFFGLHGQIIVNSVMDPIWNTLMLDNLEAYQHGKELPHII
                     TKPFMETFTVGIGGSGMTLAVVLLMAFVLKKKQYRDVGRLALAPGIFNVNEPAIFGLP
                     IVLNATILIPWVVAPLIVTTFNYLVMAAGLVPAPTGVSVPWTVPIIASGVLATNSWLG
                     GMLQVIDFVIVAFIWYPFLKVLDKQPDLDIA"
     gene            7161..8558
                     /locus_tag="ACG842_001563"
     CDS             7161..8558
                     /locus_tag="ACG842_001563"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFQ0248110.1"
                     /translation="MKTYTFPENFWWGSAASGPQTEGRVSDDGKGENIWDHWYELEPE
                     KFFDQVGPEKTSQVYTKYKEDVQLMKETGHNTFRTSIQWSHLIPEGTGEINQKAVSFY
                     RDYFTELLDNGIEPFVNLYHFDMPLPLQEKGGWLSRETVDAYVSYAAACFELFGDLVK
                     KWFTHNEPIVPVEGGYLYQFHYPNHINMKEAVQVGYHENLASAKVIKAYHEGGHDGEI
                     GIILNLTPSYPRDETNPEDVKAAKIADAFFNRSFLDPAVKGEFPQELIEIVKELAIMP
                     EIHEGDLELIRENTIDLLGINYYQPRRIKAKETPINHEHSPMPDDYFDNYDMPNKKMN
                     PYRGWEIYEKGIYDILTNTRENYGNIKCYISENGMGVEGEERFVNSEGIIEDDYRIEF
                     VTDHLKYVHQAIQEGTNCVGYHMWTCMDNWSWTNAYKNRYGFIAVDLAQDGKRTIKKS
                     GRWFKTVSEKNGFDA"
     gene            8639..9079
                     /locus_tag="ACG842_001564"
     CDS             8639..9079
                     /locus_tag="ACG842_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HFQ0248111.1"
                     /translation="MVDTYRNILVAIDGSEKAEKAFLEAITIAKRNQATLHILYVNEV
                     TGNYFGDFAFVTTSLQEELDEVAENQMKEHRNLAIEKGLTDIETYVLYGYPKTLIANF
                     HESKEPIDLIVMGATGLNAVERALVGSTTSYVVNHASCNVMVVK"
     gene            9227..9553
                     /locus_tag="ACG842_001565"
     CDS             9227..9553
                     /locus_tag="ACG842_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFQ0248112.1"
                     /translation="MISSDGIRGYNDAIILSILNKNDSYGYEISKTIRERTNDSYVIK
                     ETTLYSVFARLEKNQLIVSYPGEVTHGKKRTYYKITEIGRNLLEEKKQEWLTTKDVVD
                     QFLEGN"
     gene            9554..10303
                     /locus_tag="ACG842_001566"
     CDS             9554..10303
                     /locus_tag="ACG842_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease prefix domain 1-containing protein"
                     /protein_id="HFQ0248113.1"
                     /translation="MDTIRNYLDSLFIGVPQSTEIDKLKTDLLANMEDHYHELMGEGK
                     NEQEAIGTVISTFGSIDELLEELDVEKKHQADETETNTASIYLSEAENYWKEYRAASL
                     QVASGVLFISLSFASFLFFCSAGYVFMGISCLIFGIALAVGFFIASGMKITRLNHFLH
                     HRKIPEKVLAEAKEKEEEYQRSFGFSLIAGIGLCIFSLFPLLASLMWYMDGSIGASIF
                     FVTVGTGVFLIIYGSLVRHSYRQFTQSAYYW"
     gene            complement(10383..10838)
                     /locus_tag="ACG842_001567"
     CDS             complement(10383..10838)
                     /locus_tag="ACG842_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HFQ0248114.1"
                     /translation="MLTIYSSRTHSYHLMANWLKDHQMPYRERVFTPKQPLTRKEIFY
                     LLSLTENGFDDLLAKRSIDYKNLKDQIDQCTTSELVDLIVSHFTLLQRPIITDGKKLL
                     VGFNEDSMRLFIPVQWRRSQLKSVYNDFTWVSNLENSTDLDDEFLNYYI"
     gene            complement(11265..11540)
                     /locus_tag="ACG842_001568"
     CDS             complement(11265..11540)
                     /locus_tag="ACG842_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0248115.1"
                     /translation="MKLEDKKVILKKIKRLPDQIDGYTICSFKKDRSIKAYTQNTEKG
                     IIYFLHEEGYDTQTFMFTEKKEFHKQMKKLIEKEFPRSHKLYVNQQF"
     gene            11834..12649
                     /gene="thiD"
                     /locus_tag="ACG842_001569"
     CDS             11834..12649
                     /gene="thiD"
                     /locus_tag="ACG842_001569"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HFQ0248116.1"
                     /translation="MEDQYVQAMTIAGSDSDGSAGMQADMHTFFTRNVYGVSVMTACV
                     AGNSYGIGASVTLPTDFIDKEFELIAKDFQIRAAKTGMLADSKLIETVVKNYQKYDFG
                     PLVVDPVIVTKHGNLLLEESALQSLKEKLVPLAEVLTPNFYEAEKLTGRTLAAEEDFV
                     QAAKELQDMGAKNVMIKGHHAKNAQQETVNDYVLFESGKSFWLNAPFYPTDRINGTGD
                     SLSACIAAELAKGKTVEQAIRLAKDYVNRAIREEINVGHKYGPINHWAAGELE"
     gene            complement(12978..14429)
                     /gene="cls"
                     /locus_tag="ACG842_001570"
     CDS             complement(12978..14429)
                     /gene="cls"
                     /locus_tag="ACG842_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HFQ0248117.1"
                     /translation="MVSSIITALYLLNALIALVAILIKPRDVAAIWAWLLVLFALPGV
                     GFVLYLFFGRGLTDKKKFYLRQSDLKELENFQSFRGDTIEHYDPDMGDKDKQQFVDFF
                     SSLNRMPLTRMNSVTLLTDGQEKLDSLLQDLKKAKHSIHIEYYAFVTDNIGQQVLHVL
                     EEKAAEGVEVRILYDAFGSHGTKAKDFNRLIKNGGHVHTFVTSQRALLRFRLNYHDHR
                     KIVVIDGKISYTGGFNIANQYVNTTKKFGYWRDTHIRIFGAASSLLQLRFLTDWNVSV
                     PEEKKVGYHLNYFFKKADRDESKLADTSIQLVSSGPNNEREQIKLSFIKLITSAKKRV
                     WIQTPYLVPDESVIAALKIATASGVDVKIMIPNKPDHPFIYRATQYYARQLIKENVQI
                     LVYENGFLHAKTLIMDDEICMVGSANQDIRSYRLNFETSAVIYDPEFLEELATQFKED
                     ETHCSSMTTETVKEMSNWLLFKQQISRLFSPIL"
     gene            14770..17436
                     /locus_tag="ACG842_001571"
     CDS             14770..17436
                     /locus_tag="ACG842_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HFQ0248118.1"
                     /translation="MENKEQAQQAVYKETIDSLMQQTQSSTDGLSTTQAKEKLERDGL
                     NQLKEKPKKPTWKLFLETFKDAMVIVLLIVALIQMVMGDYIEFLVIFAVLMLNSIISV
                     VQTKKAEGSLEALKNLSAPDARVIRDGKEKTIPANELVVGDIVLLDAGDYVPADGRLL
                     DAGSLKVDEGMLTGESTSAEKEVTDITSTVPIGDRTNMVFSGTIVTYGRGKFLVTSTG
                     NNTEIGQVAGLLESTTEQVTPLQRGLDKFSKKLSLAILALSLLILGIQLFRIYLGEGT
                     GDMTADIVSAVMFAVAVAVAAIPEALQSIVTIVLSLGTNKMAKRHAIIRKLPAVETLG
                     STSIICTDKTGTLTQNKMTVVDYFLGNGDTGDFTNDPSKWTADERRLIEISVLANDAS
                     ISEDGTKLGDPTEVAFIDFSEKLNQPYQEIRNNYPRQAELPFDSDRKLMSTGHTIDNK
                     NYLFVKGGPDIVFQRSKKVLLNGEVVPMTDELMKKFQEQNEAFSTRALRVLAFAYRPL
                     DNFDLSFEDEDDLILVGLLAMIDPPRAEVTQAVTEARSAGIKTIMITGDHKTTAVAIA
                     KQIGIFEEGNLALTGTELDELTEDQLNEVLEKVTVYARVSPENKIRIVRAWQNKGHIS
                     AMTGDGVNDAPALKQADIGIAMGTGTDVAKDAAAMVLTDDNFASIVSAVEVGRNVFDN
                     IKKAISYLFAGNLGAIIAIIFALVADWSNPFTALQLLFINLVNDSLPAIALGTEKAEP
                     ATMNRPPRDPNSGIFTDKTLISVTYRGVLIGIAVIFAQWLGMQHSPEFGVAMSFSTLI
                     WCRTLQTFPARSNTQTAIQAGFFSNRSVLLAVVVCSALYCLTMIPGLKEVFSIPSAFG
                     LTEVFTCMGIALVTIVLMEITKLIIVRVQGNK"
     gene            17800..18552
                     /locus_tag="ACG842_001572"
     CDS             17800..18552
                     /locus_tag="ACG842_001572"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFQ0248119.1"
                     /translation="MNEKEWDEFAEEYYEIQQESQVTIAEDVKNYLKTEKVLPAETAA
                     DVAGGSGRFLSLAKEINRYDLIDFSSGMLENAKKEAEKNELPNVSFIKQSLEEFIQSN
                     ETYEMVFTAANPALVHLIQLEKLMKKSSKWCVIIRVVESIDDVFYPIEKQLGMIEEDP
                     NTSPVVMDQFETYLIDEGFKVKKKDFTYFTQESITEELMKLYYDAYAKDPVVTRYIRK
                     LFSEKREILSTTKLTYRLLLWSADKKRTFHDS"
     gene            complement(18574..19728)
                     /locus_tag="ACG842_001573"
     CDS             complement(18574..19728)
                     /locus_tag="ACG842_001573"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004421 - hydroxymethylglutaryl-CoA
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HFQ0248120.1"
                     /translation="MKIGIDRLSFFIPNLYLDMTELAESRGDDPAKYHIGIGQDQMAV
                     NRANEDIITLGANAASKIVTEKDRELIDMVIVGTESGIDHSKASAVIIHHLLKIQSFA
                     RSFEVKEACYGGTAALHMAKEYVKNHPERKVLVIASDIARYSLASGGEVTQGVGAVAM
                     MITQNPRILSIEDDSVFLTEDIYDFWRPDYSEFPVVDGPLSNSTYIESFQKVWNRHKE
                     LSGRGLEDYQAIAFHIPYTKMGKKALQSVLDQTDEENQERLMARYEESIRYSRRIGNL
                     YTGSLYLGLTSLLENSKSLQPGDRIGLFSYGSGAVSEFFTGYLEENYQEYLFAQSHQE
                     MLDSRTRITVDEYETIFSETLPEHGECAEYTSDVPFSITKIENDIRYYKI"
     gene            19913..22354
                     /locus_tag="ACG842_001574"
     CDS             19913..22354
                     /locus_tag="ACG842_001574"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004420 - hydroxymethylglutaryl-CoA
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0015936 - coenzyme A metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HFQ0248121.1"
                     /translation="MKEVVMIDAARTPIGKYRGSLSPFTAVELGTLVTKGLLDKTKLK
                     KDKIDQVIFGNVLQAGNGQNVARQIALNSGLPVDVPAMTINEVCGSGMKAVILARQLI
                     QLGEAELVIAGGTESMSQAPMLKQYQSETNEYGEPISSMVNDGLTDAFSNAHMGLTAE
                     NVATQFSVSREEQDRYALSSQLKAAHAVEAGVFSEEIIPVKISDEDVLSEDEAVRGNS
                     TLEKLGTLRTVFSEEGTVTAGNASPLNDGASVVILASKEYAENNNLPYLATIKEVAEV
                     GIDPSIMGIAPIKAIQKLTDRSGMNLSTIDLFEINEAFAASSIVVSQELQLDEEKVNI
                     YGGAIALGHPIGASGARILTTLAYGLLREQKRYGIASLCIGGGLGLAVLLEANMEQTH
                     KDVQKKKFYQLTPSERRSQLIEKNVLTQETALIFQEQTLSEELSDHMIENQVSEVEIP
                     MGIAQNFQINGKKKWIPMATEEPSVIAAASNGAKICGNICAETPQRLMRGQIVLSGKS
                     EYQAVINAVNHRKEELILCANESYPSIVKRGGGVQDISTREFMGSFHAYLSIDFLVDV
                     KDAMGANMINSILESVANKLREWFPEEEILFSILSNFATESLASACCEIPFERLGRNK
                     EIGEQIAKKIQQAGEYAKLDPYRAATHNKGIMNGIEAVVAATGNDTRAVSASIHAYAA
                     RNGLYQGLTDWQIKGDKLVGKLTVPLAVATVGGASNILPKAKASLAMLDIDSAKELAQ
                     VIAAVGLAQNLAALRALVTEGIQKGHMGLQARSLAISIGAIGEEIEQVAKKLREAEKM
                     NQQTAIQILEKIREK"
BASE COUNT         7198 a         3934 c         4673 g         6617 t
ORIGIN      
        1 aaaaaatgaa aaatcggtta attgtcgcat gtggaagtgg agtagcaacg agtcagacaa
       61 tcgctagtaa gatccaaagt atgttagaag atgacggcat cgcatttcct gtagaagcag
      121 ttgattacaa atcgattcaa aatgagcttc taactgctgg aatctatgtg tatgtcgcac
      181 aacctgatga ggaagtactt gaacaagcaa aagatttggg tattgaagta tttccaggaa
      241 ttccattttt aactggaatg ggtgtagaac ctatttatga cagtatcaaa gagttagtac
      301 agtaaaggag gattgttgga taaaaaagtt ggttcatagt tttatttact atatttagaa
      361 agaaagaagg tttatttatg gaagttttcc aaaccattat caattatgtt ttgaacttag
      421 gatcagccat ttttgttccc ctgattattt tgatacttgg attgttggca gggatgaaat
      481 taaaaaaagc attcatgtct gctttgactt tagggattgc ttttagcgga atgtctatgg
      541 tcattggcta tatgtctaac gcagtaagtc cagcatcaga agcattggct aaaaatacgg
      601 gaatcagttt gcctgcactt gatcttgggt ggacaggtgc agcaagcatc acatggtcgt
      661 ggagttatgc ttttatattt tttgcagtaa cgattggggt caattttgta ctgttacttt
      721 ttaattggac gaagacgtta aatgttgata tgtggaatgt ttgggggaaa gctttaactg
      781 cttatttagt ttattatgtt tccggtagtt tggctgcagg ctttcttact gcaatggtcc
      841 aagttatatt ggaattaaag cttggtgata tgtttcaaaa acatatacaa gatctcacag
      901 ggattccatt agtaacagtg actcatttta tgacatctgc cgctgtcttg ttgctgcctt
      961 tcaatatgat catggataaa attcctgctt taaataaacg agcggatacg aatgcattga
     1021 agaagaaaat tgggatcttc tctgaaaact ctgtaatggg attcattatt ggattggggc
     1081 ttggctttgc tgctgcgtat ggcgtttcag gttctcttaa tctagcgatc caaacagcta
     1141 cagcattaag tttgtttcct atgatctcta aaatgttcat gcaatctttg tctcctctag
     1201 cagacgctat gtcagaattt atgaaaaagc ggtttaagga tcgtgaagta tatatcggat
     1261 tggactggcc gatattagct ggtcgttcag aaatttgggt tactgctatt ttacttgttc
     1321 ctgtgtttat tggttatgcc attattttgc caggaaacca agtattgcca ttagcaggga
     1381 ttatcaacta ttcgattgcg gtcggcggat tacttttaac aggaggaaac ttggctcgta
     1441 tgctgatctt gggaatcatc actatgccta tttatttgta cggggccact taccttgcgc
     1501 caatactcag tgatttagca gaaaaaagcg gagcagtcga gggaatcaag aaaggacagt
     1561 tgatcacatg gtcatccata gaagggccgg agttcagggt tttatttgcg gaggcattta
     1621 ataggaatat tctcgctatt gtaggatgcg tgatttttct aggactgttt gttctacttt
     1681 ataaatatat gataaaagcg aaagtaccga gtgaacgtta tgctgaacta caaaaatagc
     1741 attgaaatgg tgggaaagta atgaaaagag aagagacaga taaaataaaa tggacagtcg
     1801 ctctttgtgg tacacttctg ctatttctat atggactctt tactcagaat atcatcatca
     1861 atctattggt catttttttc gcactagtga tctataaata tggaaatcac gttttattca
     1921 gagagtacga tgaaaaaaga aaacggaaaa tagaagaatc aatgaaaata aaagaagcta
     1981 ccaaagaaat attgagagaa aaaagtttta taaaaagata atggaggggt cattatgtca
     2041 gatggacgta tgttatttga gagagaacga caggacatgg cacgtatcgt aagagtgatt
     2101 ttcgaaagga aaaacacaaa tgtcgcagga gggaattttt cctttaaaac cactgatgat
     2161 caagggaaag actacatcat catgacgccg accatgatgt cagaagccta tctaggagag
     2221 atttctcctg cacaggttct agtagtagaa ccaagaacgc gtagaatcgt tgacggagtc
     2281 ggtcatttaa ctcgtgaaat caatatgcat gaagcgatgt atgagactaa tccagctatc
     2341 aaagctgtcc tacatgctca tgcgcctaat gcaatgttct gggcgactac tgggttgcct
     2401 atgcctaatt tgacagaagc cacagctaaa gtaaaagaga ttcctgtcat ggactttgag
     2461 cctaattgct ctgaagaact tgctgaattg acaagcgact atgtaaaaaa acagcatctc
     2521 gaattaccgc atatgttgct gctcaactct catggcgtat tgatcaacac aggtggagaa
     2581 tcaggaatcg aagctatcca taaagcactg caaatccttg atacagttga atggaatgca
     2641 gaaattgcct acaagcagac gatttttcaa gcattaggga ttactgacgg tttttattca
     2701 aaaggtgaga aaatcggggg attggaagag ttgaaacata aaacggctat ttataataaa
     2761 tctgttattg cagctggtgg agactaacct atctgacaca aaaaactacg actttcacaa
     2821 aatctgatcc agatcgatat ctgcttcagg ttgtattatg taagtcgtag ttcttttctt
     2881 ttatttagtt aagaggtggt ctgtccattc cctcgcttgc ttgatttcgt ccatcgtcaa
     2941 ctgatgacct gttacagtta gctgaatagt cacatttgct tggcgtgttt cgaattgatc
     3001 ggccaattct ttaaaggaag caggggaaac gattggatcg ttatcaccat aagaaatcca
     3061 gacggtttta tcctctaatg tggaacggct ttgatgaaca cctagagaca tcggatggaa
     3121 aaacaaccct ttatttagct cggtctcacg ctccaataat aaatgagcag cgatgtttgc
     3181 gccatttgaa tagccgacaa gcagccagtc acttaacggt atcagttctt tttcgctgat
     3241 ttctttgatt ttttcatgga gtttatctgt ttctttttcc aaactgtcca ggtcgaattg
     3301 attcaatcca ttacgcttga aaaaacgatt catcccatct tcttggaccg tgccacgaaa
     3361 agaaagcaat gtactgtttg gtgcaagaaa gtgagcgata tccagtaaag aatgttcatc
     3421 gccacctgtg ccatgaagta agatcaaatg cttgccactt gttccttttt caaaaatgta
     3481 ggtcattttg ttccctcttt tctgtttcta tagatgcaaa tgaagtagca tgaagagatt
     3541 attcacgttt tttatttgca tcacttgtat cgaaaggacg gacataagct tcaatggctt
     3601 cacgacgttc ttctaagaat ggaggaagag atagtttttc tcctgctgat tctgctggtt
     3661 catcttcaag gaatccagga ccatcagttg ctaattcaaa cagtacgtga tgagcggcta
     3721 ggaagtactc agattggaaa tagaaacgat cgacgaaacc agaatttggc aattcaatct
     3781 tattgattac ttgaatccag tattccaatg cttctttgtc tgctacacgc aatgctaagt
     3841 gatggatatt tccaaatcct tcgattgctt ttggcaagtc tgtccgatgt tcaataatga
     3901 tgcttgcacc atttccccct tcaccgattt caaataagta gaaatctcct gaatggtctg
     3961 tttctttgaa tcctaagatt ttttcaagaa tcaagcggat atgctctaat tgactgacac
     4021 ggacaaagac aggtccaagt cctgtgatcg caaactctgt cggtacattg ctgtttttcc
     4081 aaggaatgcc agctgctaca ccttgattgt tttcgtcaga gatcaattga taatactgat
     4141 tatcgaaatc ttcaaattcg atatattttt tgccgaaacg ttcattgatt gctgaatgag
     4201 taacgtcata tttttcaaaa cgttccaacc aaaagtctaa tgctgcgtca cttggcacac
     4261 ggaaagaagc acgagagatc gtatctgttc ctttttttcc ttttggctga tttggaaaat
     4321 caaagaaagt catatctgtt cctggcgcac cagtatcgtc tgtaaagtaa agatggtaag
     4381 tttcgatgtc atcttggtta actgtttttt tgattaagcg cattcctaaa atatctgtaa
     4441 aaaagcggta gatttttgta gcgctagatg tcattgctgt gacgtggtga attcctctga
     4501 tttgtgtatc catgataaat ttcctcctgt taacttacaa aaagtaagta gttgatttag
     4561 acatagtata gagggtgcat ctctattttg caattaattt gtcttctaaa gtatcaggaa
     4621 ggaatcaaga atggaaaaga ggagggacgg atcaataaaa taaaaagact tacgactagc
     4681 tgtatcgatt tcaaaaaaac tagctttaaa aagctaactt tagaatcatt acgggtggtc
     4741 ataagtcttt ttatgctgag ttattttttc atttttgagc taatgcttag gatagaatag
     4801 aatgttgatt tcacgatctc ttcttgttac ttgaaactgc ttaccactgt cacgatctct
     4861 tggtaaacgg ctttttcaca acctctttgg ttaagttgaa cgcacatcca gcacagaata
     4921 gttccgaaca tcaaatcggt ttcggctgga agaatattca atcggctgtc catctttgag
     4981 atagacgact tgctgaactt ccagtacagg atcgttttct gcacagttca aatattgctg
     5041 atcatattca tcacttttat ctgcagaaat agcacgataa gcccctgcaa aatggacatt
     5101 caattcttga tgaagatgat catagataga ggctaacaaa tgttcttttt ttaaacccgg
     5161 cacaagatgg actggcataa aggtatgctc taaaacatat ggctcgccat ctaagatcct
     5221 taaccggtga atattgtaga caggttgctc ggcatcgatc atcaatcgtt cttggatata
     5281 ttgattaggg aattcaacat caaaatgaat gatttgactc ttcaaagaac gatgtctgcg
     5341 tttcatttgg agactcaaac cttcatactc gttagcgggc gatgtgtctt tttgcataaa
     5401 tggatgattc aagattttcg ttcctgatcc tcgctgcgaa tagacaagtc cttccattgc
     5461 taagatattg atggcttttt tgattgtcat ccggctcgca ccaaattctt ttaccaagtc
     5521 gacttgatta ggcaaaaaac tatcgagagg atactctttt gaacggatac gttctcttaa
     5581 aatatttgcg atttcttcgt attttggcac gttattcacc tcttctagag tataacgaaa
     5641 gattatgctt ccgacaactc gaagtagcat ctggaacatt gtttattcga aaaaagaggt
     5701 catgacaagt ttttgtcaca accccttaat gatctattaa gcaatatcta aatctggttg
     5761 tttatctagt actttcagga atgggtacca gataaaggca acgatgacaa agtcgatgac
     5821 ttgcagcata ccaccaagcc atgaatttgt tgctaagaca ccgcttgcaa tgattggcac
     5881 agtccatgga acagagacgc cagttggtgc agggacaagt ccagcagcca ttaccaaata
     5941 attgaacgtc gttacgatta gaggtgccac aacccaagga ataaggattg ttgcattcaa
     6001 tacgatcggt agaccgaaga tcgctggttc atttacattg aaaataccag gtgctaaagc
     6061 taatcgtcca acatcacgat actgtttctt tttaagaaca aaggccatca ataaaacgac
     6121 agctaaagtc attccagagc caccaatacc aacagtgaag gtttccatga atggttttgt
     6181 gatgatgtgt ggtaattctt ttccatgttg ataagcttct aagttatcaa gcatcagtgt
     6241 attccaaatc gggtccatta cagagttgac gatgatttgt ccgtgaagtc cgaagaacca
     6301 taagaattgg acaaagaaca aagcaatcaa tgttgcaggt aaactacttc ccaaaccagt
     6361 taatggtttt tggatcactt cataaacgac gtcatgtaag ttagcattga aaataccagt
     6421 catcacagca ttgattacta agaaaatagt tagtgtcact acagcaggga tcaaagcggc
     6481 aaatgatttt gttactgcag gcggaactcc atcaggcatt ttgatctgcc agccgcgttt
     6541 cgtgattctg cagtagattt ctccagccaa gaaagcagcg atcattccaa tgaacattcc
     6601 ttttgcgcca agacgatcaa gtgaaagcac accagtcaca tcttcaccgc cagcggtttt
     6661 cattgcaaaa ggcgtaagaa tcaagaagct ggcaaaagac acggcaccac cgaaaatacc
     6721 ttcaacgtca taagattttg ataaataata gccgatacca aaagtaacaa aaaccgacat
     6781 gatgctcatt gttgcatttt gcccattacc gaataaattc gataaagttc cctttgttgc
     6841 gtcactgaaa aaaggcaagt tattgataac gacaacgatc gaaccgaaca tcgttaaagg
     6901 gaaggaaagc atgaaagcat cccgcagcac tgtaaggtaa cgattttgcc ctaataaatt
     6961 agctaagggc atgattttct cagaaagctt gtccataaaa ccgttcattt ttattcacct
     7021 caaatttatt tgcaatcgat tacatacaaa gtataacgcc aaaaatttta aatgtctagt
     7081 cattttcata aaaagtattg atttattttt tagggttcgt tatgattacg gtatcaagaa
     7141 aaaagagagg aagtaaaaaa atgaaaacct acacattccc agaaaatttt tggtggggtt
     7201 ctgctgctag cggaccgcaa acagaaggaa gagtaagtga tgatggtaaa ggagaaaata
     7261 tttgggatca ttggtatgaa ttagagcctg aaaaattctt cgatcaagta ggacctgaaa
     7321 aaacttcgca agtttacaca aaatacaaag aagatgttca gttaatgaaa gaaactggcc
     7381 ataacacatt tcgtacatct atccaatgga gtcatttgat tccagaaggg acaggtgaaa
     7441 tcaatcaaaa agctgtttct ttttatcgag attattttac tgaattgctg gataatggta
     7501 ttgaaccttt tgtgaacctt taccatttcg atatgccact accactgcaa gaaaaaggcg
     7561 gatggttatc aagagaaacg gtcgacgctt atgtttctta tgcagcagcg tgctttgaac
     7621 tgtttggaga tctggtcaaa aaatggttta ctcataatga accaatcgta cctgtagaag
     7681 gaggctactt gtatcaattc cactatccaa atcatatcaa tatgaaagaa gcggtccaag
     7741 taggctatca tgagaatcta gcaagcgcaa aggtaatcaa agcctaccat gaaggcggac
     7801 atgatggtga gatcggaatc atcttgaatc tgacaccaag ctatccgaga gatgaaacga
     7861 acccagaaga tgtaaaagcg gcgaagatcg ctgacgcatt cttcaaccga tcattccttg
     7921 accctgcagt gaaaggagag tttccacaag agttgatcga aattgtcaaa gaattggcta
     7981 taatgccaga gatccatgaa ggagacctag aactcatccg tgaaaacacg attgacctgc
     8041 taggaatcaa ttactatcag cctcgtcgaa tcaaagcaaa agaaactcca atcaatcacg
     8101 aacatagccc catgccagat gattattttg ataattacga catgcccaat aaaaaaatga
     8161 atccatatcg tggatgggaa atttacgaaa aaggaatcta tgatatttta acgaatacaa
     8221 gagaaaacta tggtaatatc aaatgctata tttctgaaaa tgggatgggg gtcgaaggag
     8281 aagaacgatt tgttaacagc gaaggaatta ttgaagatga ttaccggatc gagtttgtta
     8341 cggatcattt gaaatacgtc catcaagcaa tccaagaagg aaccaattgt gtgggttacc
     8401 acatgtggac atgtatggac aactggtctt ggacaaatgc ttataagaat cgctatggct
     8461 ttatcgctgt tgatttggca caagacggta aacggacgat caaaaaatca ggtcgctggt
     8521 ttaaaacggt ctctgaaaaa aacggatttg atgcctgata ttttttgata aaacgttcac
     8581 aaaatgtttg ttctaacact gtaatcgtgc tacactaaca gtgaaggagg cgtttacaat
     8641 ggtagatact tatcgtaata ttttagtagc aattgatggt tctgaaaagg cagaaaaagc
     8701 atttcttgag gcaatcacga ttgccaaacg aaatcaggca acgttgcaca tcctgtatgt
     8761 caatgaagta acaggcaatt actttggtga ttttgcattt gttacaacaa gccttcagga
     8821 agaattggat gaggttgcgg aaaaccagat gaaagaacat cgaaacttgg caattgaaaa
     8881 aggcctgaca gatatcgaga catatgttct ttacggttat ccaaagacac tgattgctaa
     8941 tttccatgaa tcgaaagaac caatcgatct gattgtcatg ggggcgacag gtctgaatgc
     9001 ggtagaacgt gcactagtag gttccactac ttcctatgta gtcaaccacg catcatgtaa
     9061 cgtcatggtt gtaaaataaa taaattaagg gaacagaaag caaaaaagca taacgcttct
     9121 tttgctttct gttcctttta tttttttgta aaaaattact ttacctgatg aagtaatcat
     9181 gctattctaa ttacaacgac agatggagta atatggagga aagaaaatga tttcaagtga
     9241 tggaatacgc ggatacaatg atgcgattat tttatcgatc cttaacaaaa acgattctta
     9301 tggatatgaa atttctaaaa cgattcgtga gaggacaaat gattcctatg tgatcaaaga
     9361 gacaacactt tactccgttt ttgcccgttt agaaaagaat caactgattg tttcgtatcc
     9421 tggagaagtg actcatggaa aaaagcggac atattataaa atcactgaaa tagggcgtaa
     9481 tcttttagaa gaaaaaaagc aagagtggct gacgacgaaa gacgtagtcg atcaattttt
     9541 ggagggaaat taaatggata caatccgtaa ttatttagac agtcttttta tcggagtacc
     9601 tcaaagtacc gagatagata agctgaaaac tgatttgttg gcaaatatgg aagaccatta
     9661 tcatgaattg atgggagaag gaaaaaatga acaggaggcc atcggtacag taatttcgac
     9721 atttggttca atcgatgaat tattagaaga actagatgtc gaaaaaaaac atcaagctga
     9781 cgagacggaa acaaacacag cttctattta tttaagcgaa gcagaaaact actggaaaga
     9841 atacagagcg gcaagtctcc aagtggcatc aggtgttcta ttcattagct tatcttttgc
     9901 aagttttctt ttcttttgtt cagcaggata tgtgttcatg ggtatttctt gtctgatttt
     9961 cggtatagca ttagctgtcg gtttttttat tgcttctggt atgaaaatca cacgtttaaa
    10021 tcactttttg caccatcgga aaatacctga aaaagtatta gcagaagcaa aagaaaaaga
    10081 agaagagtat cagcgaagtt ttggcttctc actgattgca ggaattggat tatgtatttt
    10141 ttcacttttc ccacttttgg cttctttaat gtggtatatg gatggtagta tcggtgcctc
    10201 tatatttttt gttacggtag gaacgggtgt cttcttgatc atttatggca gtctagtccg
    10261 tcattcttat cgtcaattca ctcaaagcgc ttattactgg tgaatgtatt cagatttgta
    10321 aaaatattct atttatccgg cgtagaaaaa aagactgtga cacgcttttg tcacagtctt
    10381 ttttatatat aataatttaa aaattcatca tctaaatccg tagaattttc tagattgctt
    10441 acccatgtaa aatcgttata gacagatttc agttgacttc tgcgccattg tacagggata
    10501 aaaagacgca tgctatcttc attgaatccg actaataatt tctttccgtc agtaatgatg
    10561 ggacgttgta acaaagtaaa atgcgagacg atcagatcga caagctctga ggtcgtacat
    10621 tggtcgattt ggtctttcaa atttttataa tcaatcgagc gtttagctaa tagatcgtca
    10681 aatccgtttt ctgttaatga caataaataa aatatctctt tccttgttaa aggctgttta
    10741 ggtgtgaaaa ctcgttctct gtaaggcatt tgatggtctt tcagccagtt tgccatcaga
    10801 tggtaactat gtgtgcgaga agaatagatc gtaagcataa aaaaactcct taaatagtcc
    10861 attaatttgt tagatctgtt gagataaata ataggtaagg agctgtgcat aagcattgat
    10921 caaaacaacc caaacatcat tagaaataac tttctaaagt cttaaacttg cgacgagaag
    10981 caactagact aatagaaaaa atgtcttagc catttggctt cctgtttgtt tttttgctta
    11041 tgctgcagct cctcgggaaa gggttctaac aatttgaaga aggttgatga ttgatctagt
    11101 ttaaattgtg tgaaatttga aatctggtgt ttttactaga tgaatcacct aacaactatg
    11161 attataactt atagaacttc tgttttgcat tcggttttgt tatagttgtt ataaagtttt
    11221 attttattac agaacatttt taattaataa agaacagtta gaagttaaaa ttgctgattc
    11281 acatagagtt tatgacttct tggaaattct ttttcaatca gttttttcat ctgtttatga
    11341 aattcttttt tttcagtaaa cataaaggtt tgggtatcgt aaccttcttc atgtaggaaa
    11401 tagattattc ctttttctgt gttttgcgta taagctttta tactcctgtc ttttttgaaa
    11461 gaacagatcg tatagccatc tatttggtct ggtagtcgtt tgattttctt caaaatgact
    11521 tttttatcct caagtttcat ttttttcacc tgactttatt tttttcatat ggaaatcata
    11581 gcgcctttgg ttatatgaaa acaagaagga tactgaaata tatataaatc aaatagtggt
    11641 tatttattat tagaatagcg aatacaaata aacgaccact attttctgat ttattgcaaa
    11701 tgttttttca aataaatttg attaccaaga aaaggtagtg gtatatttac tgagggatac
    11761 tttgaaaaac atatgataga aacagtaaca gtattatgaa aaatcttttt ataaaaatgg
    11821 aggaatcgat ttgatggaag atcagtatgt acaagcaatg acgatcgcag gatcagatag
    11881 cgatggaagc gcagggatgc aagcagatat gcatacattc tttacaagaa atgtttacgg
    11941 tgtttctgtc atgaccgcat gtgttgcagg taattcttac ggaatcggag caagtgtgac
    12001 attgccaaca gattttatcg acaaagaatt tgaactgatt gcgaaggact ttcaaatacg
    12061 tgcagctaaa acaggtatgc tagcagattc aaagttgatt gagacagttg taaaaaatta
    12121 ccaaaaatat gattttggac cattagtcgt ggatcccgta atcgtaacga aacatggaaa
    12181 tcttttacta gaagaatctg ctttgcaaag cttgaaagaa aagcttgtcc ctttggccga
    12241 agtactgaca cctaattttt atgaagcaga aaagctgact ggacggacat tagcagctga
    12301 agaagatttt gtgcaagcag caaaagaact gcaagatatg ggtgcaaaaa acgttatgat
    12361 caaaggacat catgctaaaa atgcgcagca agaaacagtc aatgactacg tattgttcga
    12421 atcaggaaaa tcattttggt taaatgcacc attttatcct accgaccgga tcaacggaac
    12481 tggcgattcg ctatctgcat gtattgcagc tgagttagcg aaaggaaaaa cagttgaaca
    12541 agcgatccga ttggcaaaag actatgtaaa ccgtgcaatt cgtgaagaaa taaatgtagg
    12601 gcacaaatat ggccccatca atcattgggc agctggtgaa ttagagtaaa agaaagaagt
    12661 tgtgaaaaag ttgccctgca tcaagtaata agaacagcca agtaaaaaca gcttcttatg
    12721 tttttaactt ggttggactt attaccgcag atgcaagctt ttgaacaccg tttaagaaag
    12781 aggtcgtgag cctgccgtcc agctccgagt cacaaggaac aagttcaaaa aacagcttct
    12841 catgtttgtt gaatattgtg acttgtgacc gaggagttgg caggagaaca ccgtttgttc
    12901 ggaagaaggg actgtgacaa gatttttgtc acagtccctt taatcttagt atatttttct
    12961 ctacttgaat aaaaaaatta taggattgga gaaaataatc gagaaatttg ttgcttgaat
    13021 aatagccagt tagacatttc cttgactgtt tcagttgtca tggatgaaca atgtgtctca
    13081 tcttctttga actgagtagc aagttcttct aaaaactcag gatcgtaaat gacagcactt
    13141 gtttcaaaat tcaatcggta gctacgaata tcttgatttg ctgaacctac catgcagatt
    13201 tcatcatcca ttatcaatgt ttttgcatgg aggaagccgt tctcatagac aaggatttgt
    13261 acattttcct tgatcagctg ccgagcataa tattgtgttg ctcgataaat aaaaggatga
    13321 tccggtttgt tgggaatcat gattttcaca tctacaccag aggcagttgc gatttttaaa
    13381 gcagcaatga cactttcatc aggaacaagg taaggtgtct gtatccaaac acgtttttta
    13441 gcagaagtaa tcaatttgat aaatgaaagc ttgatttgtt ccctttcgtt attcggtccg
    13501 cttgaaacaa gctggatgga tgtatcagca agcttagatt catctcgatc tgctttttta
    13561 aagaaataat tcaaatgata gccgaccttt ttttcttcag gtaccgagac gttccagtct
    13621 gttaagaagc gaagctggag caaagaagaa gcggcaccga aaatccgtat atgcgtatcg
    13681 cgccaatagc cgaatttttt tgttgtattt acatattgat tggcaatatt gaaaccaccg
    13741 gtataactaa tctttccatc gatcacaacg atttttcggt gatcatggta attcaatcgg
    13801 aaacgaagta atgccctttg tgaggtaaca aatgtatgga catgtccacc atttttgatt
    13861 agacgattga aatcttttgc ttttgtgcca tgagagccaa atgcatcata taatattcga
    13921 acttccacgc cttctgcggc tttttcttct aaaacatgta agacttgctg gccgatatta
    13981 tctgtcacaa atgcgtaata ttcgatatgg atcgaatgtt tggctttttt tagatcttga
    14041 agcagtgaat ccaatttctc ttgtccgtct gtgagaagag tgacagaatt cattcttgtc
    14101 agcggcatac gatttaatga agagaagaag tcaacaaatt gctgtttgtc tttatcgccc
    14161 atgtcaggat cgtaatgttc aatggtatct cctctaaaag actgaaagtt ttctaattct
    14221 ttcaagtcac tttgtcggag atagaatttt ttcttatccg ttaatccacg tccgaaaaat
    14281 aaatataata caaagcccac cccaggaagg gcaaacaata ctaagagcca tgcccaaatg
    14341 gctgctacat ctcggggttt gatcaaaata gccaccaaag caataagtgc atttaataga
    14401 taaagggcgg ttataatact agataccaca aaaatgttcc tccttacata caataacatt
    14461 agtatagcat gatagaagaa agaagcctac caaggtgaat atacttttca ctcctttcat
    14521 gaaaatgtag gtaaatcggg ctaaagtagt tgctttttgt aggttaataa ggtaggatat
    14581 tagtacagtt gaatatctcc ttaggggagt aaccggcaac gtttgttgcg ataatatcaa
    14641 cagtaagtta accaaatggt tttctggtat tgtatgaaat ggtgagacct aagacaaatg
    14701 cgtatttgtc ttaggtctct tttttttcag acaggactag ggccaagatc aaaaaatggg
    14761 ggatgaaatt tggaaaacaa ggaacaagca caacaagcgg tctataaaga aacgatcgac
    14821 tcgttgatgc agcaaacgca gagctcaacc gatggattga gcaccacgca agcgaaggaa
    14881 aaattagaaa gagatggctt gaaccagcta aaagaaaagc cgaagaagcc aacttggaaa
    14941 ttgttcttag aaactttcaa agatgcaatg gttatcgtat tgctgattgt cgcactgatt
    15001 cagatggtca tgggtgacta catcgaattc ttagttattt ttgcagtatt gatgttgaat
    15061 tctataatca gtgttgtcca aacaaaaaaa gcagaaggtt ctttagaagc gttgaaaaac
    15121 ctttcagcgc ccgatgctcg agtgattcgt gatggtaagg aaaaaacgat tccggcaaac
    15181 gaacttgtgg taggagatat cgtgttgtta gacgcaggcg attatgtacc tgctgatgga
    15241 cgtttactag atgcgggatc attaaaagta gatgaaggta tgctgacagg agagtcgact
    15301 tctgcagaaa aagaagtaac ggatatcaca agtacggtac caatcggaga tcgtacaaat
    15361 atggtattca gtggaacgat cgtaacttac ggacgcggta aattcttagt gacttcaact
    15421 gggaataata cggagatcgg acaagttgct ggccttttgg aatcaacaac tgagcaagtc
    15481 actccgttac aacgaggtct ggataaattc agtaaaaaac taagtttggc tattttggca
    15541 ttgtctttat tgattttagg tatccaactg tttcgtattt atcttggaga aggtacagga
    15601 gatatgactg cagatatcgt cagcgcagtg atgtttgccg tagccgtagc cgtagctgct
    15661 attccagaag cattacagtc tatcgtaaca atcgtgttat ctttgggaac gaataaaatg
    15721 gcaaaacgtc atgccattat tcggaagctt ccagcagttg aaacacttgg ttcgacaagt
    15781 atcatctgta cagataagac tggtacattg acacaaaaca aaatgacagt tgttgactac
    15841 tttttaggta atggcgatac tggggatttc acaaatgacc cttcaaaatg gacagctgac
    15901 gaacgtcgct tgatcgaaat cagtgtttta gcgaatgatg catcgatcag tgaagacggt
    15961 actaaattgg gcgaccctac ggaagtagcg tttatcgact ttagtgaaaa attaaaccag
    16021 ccttatcagg aaatccgaaa caactaccca agacaagcag aacttccttt tgactcagat
    16081 cgaaaattaa tgtctactgg acacacaatt gataacaaaa attacttgtt tgttaaaggc
    16141 ggaccagata tcgtcttcca acgcagtaaa aaagtattgc taaatggtga agtcgttccg
    16201 atgacggatg aattgatgaa aaaattccaa gagcaaaatg aagctttttc aacacgcgca
    16261 ttacgtgtac tagcttttgc ttaccgtcca cttgataatt ttgatttatc atttgaagat
    16321 gaagacgatt tgatccttgt tggtttgctt gctatgatcg atcctccacg tgctgaagtg
    16381 actcaggcgg taacagaagc aagaagtgca ggaatcaaaa caatcatgat tactggtgac
    16441 cacaaaacaa cagctgttgc tatcgcaaaa caaatcggga tttttgaaga aggaaacctg
    16501 gctttgaccg ggacggaact agatgaattg acagaagatc aactaaacga agttttagaa
    16561 aaagtcactg tttatgcacg tgtttcacct gagaataaaa ttcgtatcgt tcgtgcttgg
    16621 caaaataaag gtcatatctc agctatgaca ggtgatggtg taaatgacgc accagcattg
    16681 aaacaagcag acatcggaat tgctatggga acgggtacag atgtggcaaa agatgcagca
    16741 gcgatggtat tgacggatga taactttgct tctattgtca gcgcagtaga agtaggacgt
    16801 aatgtgtttg ataatatcaa gaaagctatt tcttatctat ttgcaggaaa tttaggtgcg
    16861 atcattgcga tcatctttgc cttagttgct gactggtcca atccatttac agcattacaa
    16921 ctgttgttca tcaatttagt caatgattca ctgccagcaa ttgcattagg tacagaaaaa
    16981 gctgaacctg ctacgatgaa ccgcccgcca agagatccaa attcaggaat ctttacggac
    17041 aaaacattga tttctgtaac gtatcgtggt gtgctgattg ggattgctgt tatctttgct
    17101 caatggcttg gcatgcagca ttcacctgaa ttcggtgtag ccatgtcatt ttcaacattg
    17161 atttggtgtc ggacattgca aacattccca gcacgttcaa acacacaaac agctatccaa
    17221 gcagggttct tctctaacag aagtgtctta ctagctgtag ttgtatgtag cgcgctttac
    17281 tgcttaacaa tgatcccagg actgaaagaa gtcttctcta tcccatcagc atttggattg
    17341 acagaagtct ttacatgtat gggaatcgca cttgtgacaa ttgttttgat ggaaatcact
    17401 aaattgatca ttgtacgagt acaaggaaat aaataaaacg aatcaacgag gttgtgagcc
    17461 tgccgtttaa ttctgagtca caaggtgcaa tttcaacaaa cagcttctca tgtttgttga
    17521 atattgtgac ttgtgaccga agagttggca ggagaacacc gtttaaggaa gaagttgtaa
    17581 aatcagcatt ttgagctaat actgagggtc atctgattgg acactgttta ttcggaaaga
    17641 gggactttga cacgactttg gtcacagtcc ctttttttat ttatgataga cggaaagcca
    17701 atcattttat tattcatcaa aagagataga atagataaaa gaggagaatg atttattccg
    17761 aaaagtgaaa acatataaca aaaaattaga ggaaatcaaa tgaatgaaaa agaatgggat
    17821 gaatttgctg aagaatatta tgaaatccag caagaatcgc aagtaactat tgctgaagat
    17881 gtaaaaaatt atctgaaaac ggagaaagtc ctgcctgctg aaacagcagc agacgttgct
    17941 ggaggatctg gcagatttct ttccttagca aaagaaatta atcgctatga tttgatcgat
    18001 ttttcttcag gaatgctaga aaatgcaaaa aaagaagcag aaaaaaatga gttgccaaat
    18061 gtttccttta tcaaacagtc gttagaagaa tttatccaat caaatgagac atatgaaatg
    18121 gtttttacag cagcaaatcc agcattggta catcttattc aattagaaaa attgatgaaa
    18181 aagtcaagca aatggtgcgt gataatcaga gtagtggaat caatcgatga tgttttttat
    18241 cctatcgaaa agcaactagg aatgatagaa gaagatccta atacatcgcc agttgtaatg
    18301 gatcaatttg aaacatactt aattgatgaa ggattcaaag tgaagaaaaa agattttact
    18361 tattttacac aggaatcaat tactgaagaa ttgatgaagc tatactatga cgcgtacgca
    18421 aaagatcctg ttgtgaccag gtacattcgc aaactattca gtgaaaaacg agaaatcctt
    18481 agtacgacta agctgaccta tagactgctc ctttggtccg cagataaaaa aagaaccttt
    18541 cacgactcgt gaagggttct ttttatgatt ttattatatt ttgtagtaac gaatatcatt
    18601 ttcgatcttt gtaatcgaaa aaggtacatc tgaagtatat tctgcacatt ctccgtgttc
    18661 aggaagcgtc tcactgaaaa tcgtttcata ttcgtcaaca gtgatccgtg ttcgagaatc
    18721 tagcatctct tgatgagatt gtgcgaagag atattcttga tagttttctt ccagataacc
    18781 tgtaaaaaat tcacttactg caccagaacc gtagctgaat agaccgatgc gatctcctgg
    18841 ctgtagtgat ttcgaatttt ccagtagaga agttagcccc aggtataatg aaccagtgta
    18901 aagattaccg attcgtcggc tgtaacggat gctttcttca tagcgagcca taagacgttc
    18961 ctgattttct tcgtctgttt ggtctaatac gctttgcaat gccttttttc ccatcttagt
    19021 atacggaata tggaaagcaa tcgcctgata atcttcgagt cctcgacccg acaattcttt
    19081 atgtcgattc caaacttttt ggaatgattc gatatacgta gaattagata aaggaccatc
    19141 aacaacagga aattcgctat aatctggacg ccagaaatca tagatatctt ctgtcagaaa
    19201 tacgctgtcg tcttcaatcg ataaaatacg cgggttttga gtgatcatca tcgcaacagc
    19261 accgacacct tgcgtcactt caccaccgct tgccaagctg taacgagcaa tatcacttgc
    19321 tatgactagt acttttcgtt ctggatgatt tttgacatat tctttcgcca tgtgcaaagc
    19381 tgcggtgcca ccgtagcaag cctcttttac ttcaaaagaa cgagcaaaag attggatttt
    19441 cagtaaatgg tggataatta ccgcactcgc ttttgaatga tcaatcccgg attctgttcc
    19501 gacgatgacc atgtcgatta gctcacggtc tttttctgtt acgattttgc tggcagcatt
    19561 tgctcctagt gtaatgatgt cttcatttgc acgattgact gccatttggt cttgaccaat
    19621 ccctatatgg tattttgcag gatcatcccc acggctttct gccaactctg tcatatctaa
    19681 atataaatta ggaataaaaa aggaaagacg atcaatccct attttcattt caacacctct
    19741 ttacttatga taaaaaactt actttactaa ggtatcataa aaagattaaa agaaatataa
    19801 aaaagagaat atttaaggat tgttaaaatt taatcttaga aaaaaataac tggaacaaga
    19861 gctatttgtt tttacagcaa agaatggtat atttttgtag gaggtggctt acttgaaaga
    19921 agtcgttatg atagatgctg caagaacgcc gataggaaag tatcgcggaa gtttaagtcc
    19981 atttactgca gttgaattag gcacacttgt gactaaagga ttattagata aaacaaaact
    20041 gaaaaaagat aaaatcgatc aagtaatatt tggaaatgtc ctgcaagctg gtaatggtca
    20101 aaatgttgcc agacagattg ctttgaacag cggtctccct gtagatgtcc cagcaatgac
    20161 gatcaatgaa gtttgcggat ctggtatgaa agcagtgatc ttagcacgcc agctgatcca
    20221 attaggtgaa gcagaacttg tgatagccgg cggaacagag agtatgtctc aagcacctat
    20281 gctgaaacag tatcagtcag aaacaaatga atatggtgaa ccaatttcca gtatggtcaa
    20341 cgacggattg actgacgcat tttcaaatgc acatatggga ttaaccgcag agaatgttgc
    20401 aacacaattt tctgtgagca gagaagagca ggatcgctat gccttgtcgt cccagttgaa
    20461 agcagcacat gctgtcgaag ccggtgtatt ttctgaggag atcatcccag tcaagatttc
    20521 tgatgaagac gtgttatctg aggatgaagc agttcgtgga aatagtacat tggaaaaact
    20581 gggcacgtta cgtacagtat tctcagaaga aggaactgta acagcaggaa atgcttcccc
    20641 gttgaatgac ggtgcctctg tggtgatcct tgcatccaaa gaatacgcag aaaataataa
    20701 tctgccttat ttagcaacca tcaaagaagt agcggaggtc ggtattgacc caagtatcat
    20761 gggaatcgct ccaatcaaag ccattcaaaa attgacagat agatcaggaa tgaatttatc
    20821 aacaatcgat ctttttgaga tcaatgaagc atttgcagct tcttctatcg tagttagcca
    20881 agaactgcag ttagatgaag aaaaggtcaa tatatatgga ggagcaatcg cattaggcca
    20941 tccaattggt gcaagcggtg cacgtatctt gactacatta gcttacggat tgcttcgtga
    21001 acagaagaga tacggtattg cttcattatg tattggtgga ggtttaggct tggcagtctt
    21061 gttagaagcg aatatggagc aaacacataa ggatgtgcaa aaaaaaaagt tttatcagct
    21121 tacgccatct gaacggcgca gtcaattaat agaaaaaaat gtattgacac aagaaactgc
    21181 gttgattttt caggaacaaa cactttctga agagctctcc gatcacatga tcgaaaacca
    21241 agtgagtgaa gtggaaatcc caatggggat cgcgcagaat tttcaaatca atggcaagaa
    21301 aaaatggatt ccgatggcaa cagaggaacc atctgtcatt gcagctgcaa gtaatggtgc
    21361 aaaaatctgt ggaaatatct gcgcagaaac acctcagcgg ctgatgcgag ggcaaatcgt
    21421 tctatcagga aaatcggagt atcaagcagt gatcaatgca gttaatcatc gaaaagaaga
    21481 gctgattctt tgtgcaaatg aaagttatcc ttcaatcgtg aaacgcgggg gcggtgttca
    21541 agatatctct actcgggaat tcatggggag tttccatgca tacttatcta ttgatttttt
    21601 agtggatgta aaagatgcaa tgggcgctaa catgatcaac tcgattcttg agagtgtggc
    21661 aaacaagtta cgggagtggt tccctgaaga agagattcta ttcagtattt taagtaattt
    21721 tgctacagaa tcattggctt ctgcctgttg tgaaatccca ttcgaacgct tagggagaaa
    21781 caaagaaatc ggtgaacaaa ttgccaaaaa aatccaacag gcaggggaat atgcgaagct
    21841 tgacccttac cgtgcagcaa cgcacaacaa agggatcatg aatggtatag aagctgtagt
    21901 ggcagctaca ggaaatgata cacgtgcggt tagcgcttcg atccacgcct acgcggcaag
    21961 aaatggatta taccaaggat taacagactg gcagatcaaa ggggataaac tagttggaaa
    22021 actaacggtg cctttggctg ttgccacagt aggcggagct tccaatatcc tgccaaaagc
    22081 aaaagcatct ttagctatgt tagatattga ttcagccaag gaactagcac aagtaattgc
    22141 tgcagtaggg ttagctcaaa acctggctgc tttgcgtgca ttagttacag aaggaatcca
    22201 aaaaggccac atgggacttc aagctcgttc attagcgatc tcaattgggg ctattggtga
    22261 ggaaatcgag caagttgcga aaaaattacg tgaagcagaa aagatgaacc agcagacagc
    22321 tatacaaata ttggaaaaaa tccgggaaaa ataaaaaagc aatatcttta gaagaataaa
    22381 gcaagattta cagttatttt atttactgca gatcttgctt tt
//
LOCUS       DAZKLM010000046        22351 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3764
            SAMEA115706913-rid22582563.denovo.046, whole genome shotgun
            sequence.
ACCESSION   DAZKLM010000046 DAZKLM010000000
VERSION     DAZKLM010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706913
            Sequence Read Archive: ERR13196647
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22351)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22351)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287823.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287823.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,004
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,849
            CDSs (with protein)               :: 2,849
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 41 of 96
            Pseudo Genes (incomplete)         :: 52 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 15 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22351
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706913-rid22582563.denovo.046"
                     /isolate="VRE3764"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(530..2026)
                     /gene="lysS"
                     /locus_tag="ACG842_001575"
     CDS             complement(530..2026)
                     /gene="lysS"
                     /locus_tag="ACG842_001575"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HFQ0248122.1"
                     /translation="MTQEQQHQVEDLNDQMLVRRQKMEQLREDGIDPFGKRFERTHNS
                     EELHELFDPRTKEELAEMGLTASVAGRMMTRRGKGKAGFAHLQDREGQIQIYVRKDQV
                     GDEAYEVFKHADLGDFFGVTGQIMKTDTGEVSIKASEITILSKALRPLPDKYHGLTNI
                     EQRYRQRYLDLISNRESFDRFMKRSQIISEIRRYLDGNGYIEVETPVLHNEAGGAAAR
                     PFITHHNALDIDLYLRIALELHLKRLIVGGMEKVYEIGRVFRNEGIDTTHNPEFTMLE
                     AYTAYTDFNDVMNLTEGIIRNAAEKVLGTAKITYDGQAVDLESDFKRIHMVDAIKEQT
                     GVDFWQEMTLEEALALAEKHNVEVTEAMGVGHVINEFFETFVEETLTQPTFVYGHPVE
                     VSPLAKKNPEDPRFTDRFELFIVGREFANAFTELNDPIDQRERFEAQEKERELGNDEA
                     HGVDEDFLEALEYGMPPTGGLGIGIDRLVMLLTDAQSIRDVLLFPTMR"
     gene            complement(2137..3141)
                     /gene="dusB"
                     /locus_tag="ACG842_001576"
     CDS             complement(2137..3141)
                     /gene="dusB"
                     /locus_tag="ACG842_001576"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HFQ0248123.1"
                     /translation="MEHTWKIGNVEIPNRVVVAPMAGITNSAFRVTVKEFGAGLVVCE
                     MISDQGIHFRNKKTLEMLHIEETEHPLSLQIFGGNKDSLVEAAQFVEENTAADIIDIN
                     MGCPVNKVIKAEAGAKWLLDPEKVYEMVQAVSSAVNIPVTIKMRIGWDEEHVFAVENA
                     LAAQSAGASAIAMHGRTRVQMYEGKADWEVLKEVKKHLTIPFMGNGDVKTPEDAKWML
                     DYVGADGVMIGRAALGNPWMIHRTQHYLETGELIPEPTPAEKIATAKLHLQRLADLKG
                     EKVACREFRTHAAYYLKGIPRAAKVKVAINQTEKQQEINDLLDQFVEKAEKVLLKQTL
                     "
     gene            complement(3142..4029)
                     /gene="hslO"
                     /locus_tag="ACG842_001577"
     CDS             complement(3142..4029)
                     /gene="hslO"
                     /locus_tag="ACG842_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HFQ0248124.1"
                     /translation="MKDYLVKALAHDGFVRAYAVQATNTVAEAQRRHDTWHTSSAALG
                     RTLVGSLLLGATLKGEDKLTVRIQGDGPATGIVVDANGKGDVKGYIKNPHLSLPLNSE
                     GKIDVRGAVGTQGMFTVTKDLGLKEPFSGQTPIVSGEIAEDFTYYLAISEQIPSAVGL
                     SVLVNTDDSIKTAGGFMIQIMPGADEETISKIEEQLKTTPRISTLLDEGQTPEEILKG
                     LLGTEELNILEKMPVQFKCDCSKEKFAEAIIALGAEEIQAMIDEDHGAEAVCSFCNNK
                     YKYSEEELDGLKKEALGGI"
     gene            complement(4246..6357)
                     /gene="ftsH"
                     /locus_tag="ACG842_001578"
     CDS             complement(4246..6357)
                     /gene="ftsH"
                     /locus_tag="ACG842_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HFQ0248125.1"
                     /translation="MNKKNNGMMKNALYYVLVILAMVMVVYFIFGNNGQRSSDIEYSK
                     FTEQLQAGKIKEFEVQPANGVYKITGEYKEQQTVANSGGLAILGSTDTTTTHFTTIIL
                     PNDTTLSQVTELAKENKVATTVKEESSSGIWVSLLLSFLPIVIIIFFFYMMMGQQGGG
                     GGGGGRVMNFGKSKAKEADKKANRVRFSDVAGAEEEKQELVEVVEFLKDPRRFVELGA
                     RIPAGVLLEGPPGTGKTLLAKAVAGEAGVPFYSISGSDFVEMFVGVGASRVRDLFETA
                     KKNAPAIIFIDEIDAVGRQRGAGMGGGHDEREQTLNQLLVEMDGFDGNEGVIVIAATN
                     RSDVLDPALLRPGRFDRQILVGRPDVKGREAILRVHARNKPMADDVDLKVVAQQTPGF
                     AGADLENVLNEAALVAARRNKKKIDASDIDEAEDRVIAGPAKKDRVISKKEREMVAYH
                     EAGHTIVGLVLSRARVVHKVTIIPRGRAGGYMIALPKEDQFLMTKEDMFEQIVGLLGG
                     RTAEEIIFNVQSTGASNDFEQATALARSMVTEYGMSDRLGPVQYEGNHQVFVGRDYGQ
                     TKAYSEQVAFEIDQEVRKILMEAHDKAREIIEAHRAQHKLIAEKLLEFETLDAKAIKS
                     LFEHGVMPEGTESNDYPSEKEAKTFEEAKRALEEKDAEKQAEEKRDFEEAKHELNEEN
                     KAEHDSDSQQSEDNHSDDQYK"
     gene            complement(6447..6992)
                     /gene="hpt"
                     /locus_tag="ACG842_001579"
     CDS             complement(6447..6992)
                     /gene="hpt"
                     /locus_tag="ACG842_001579"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HFQ0248126.1"
                     /translation="MLEKDIERILISQEEIQVRCKELGKELTEIYQNTNPLVVGVLKG
                     AVPFMADIVRSIDTYLELDFMDVSSYGNATVSSGEVKIVKDLDTNVEGRDLLIVEDII
                     DSGRTLAYLVDLFKYRKAKSVKIVTLLDKPEGRVVNIEADYVGFNVPNEFVVGYGLDY
                     AEAYRNLPYIGVLKPSVYQSN"
     gene            complement(6992..8437)
                     /gene="tilS"
                     /locus_tag="ACG842_001580"
     CDS             complement(6992..8437)
                     /gene="tilS"
                     /locus_tag="ACG842_001580"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HFQ0248127.1"
                     /translation="MNVEKGGKKAPLLQQSFIRHGNEQGFWQQGSRILAAVSGGVDSM
                     VLLHLLLQVQRQIGFSLGIVHINHQLREESAEEAAYLRSYCEEKNLPLYVSIWEDPAK
                     SAIETEARNFRYQEFTRVMEEGQYDTLMTAHHGDDQIETVLMKIVRGGQLNTFSGIKE
                     VQPFATGRLIRPLLSFSKEDLYTYAAESQLVYFEDQTNQLLDIQRNRLRHLVVPQLKQ
                     ENTQALRHFQQFSQQIQWADQVIQKYMGQLIEKEVEQWEEQFRFSAEMIEEMTDAERY
                     YFFYSFFDKAFSKTGIILKEQQMESILDQWQQKKSQWQLSIGNNWILKREYHFVVLSK
                     KEKQATSQTEDQRLLLIPEQGIYLNETEWIGLVKPENAGDIKATDSWSLFSHDIWIPA
                     DVQLYVEKRKAGDRIKLTENLTKKVSRYFIDQKVPNSQRQHAWIITDAKRNIYSLIPF
                     AFSYLSIREETDKIHYILLYKYQKEAIGRRT"
     gene            complement(8557..9027)
                     /locus_tag="ACG842_001581"
     CDS             complement(8557..9027)
                     /locus_tag="ACG842_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 domain-containing RNA-binding protein"
                     /protein_id="HFQ0248128.1"
                     /translation="MSIEVGAKLQGKVSGITNFGAFIDLGAGKTGLVHISEVSNGYVK
                     DIHDVLSVGDEVTVKVISVNDDGKIGLSIRKAQEQPAESGEESRPKKYQQKDPRDNRN
                     RPAQKKSFSRSQPSAQKEDFDSLMSSFLKDSDDRLSSLKRNTEGKRGGRGGRRS"
     gene            complement(9073..9498)
                     /locus_tag="ACG842_001582"
     CDS             complement(9073..9498)
                     /locus_tag="ACG842_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HFQ0248129.1"
                     /translation="MTKKSNKIAALDTEYAKKKYAEFQQQQRQLIFRRRRLAAIFVGA
                     LVIFAIIGVQIFHDMQRMHQLNELRVEANTEMKEVNADVDQLHQDVSLLKDDEYVAKL
                     ARSKFFYSKDGEKVYPILESNTDSSTNETDQSTSSTTAK"
     gene            complement(9565..9834)
                     /locus_tag="ACG842_001583"
     CDS             complement(9565..9834)
                     /locus_tag="ACG842_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HFQ0248130.1"
                     /translation="MRLDKFLKISRIIKRRSVAKEVADKGRIQINGKLAKSSSDVKVG
                     DILKVQFGNKTLEVKITELHESTKKEDAQKMYEIISENRTETLEK"
     gene            complement(9845..11440)
                     /locus_tag="ACG842_001584"
     CDS             complement(9845..11440)
                     /locus_tag="ACG842_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HFQ0248131.1"
                     /translation="MDHKEMRRVMQGAFVLTIASFIAKVLSALYRIPLQNLVGDEGFY
                     VYQQVYPIYGIAMTLALSGLPQFISKYVAERKNPFERKQALQNLYPLVFWTGVFLWLF
                     VFAFSSWIAWMMGDIQLKPLIQVVSFTFLLMPGLSFYRGNFQGRFLMEPTAVSQVVEQ
                     FVRVFVIVCSAFAFRKLGWTVYQTGTAAMSGAVFGGLCAYGILHYYEQKIHGGALSFR
                     RFPLFKQPSKPLIRRFLVEGGLVSIYSGLLIFFQLIDSFFVKNALEVYGLSEYGAKIA
                     KGVYDRGQPLVQLGLVIATALSATFLPALTRHLTNRIYRQFLQTAKIYLRLTTALALA
                     ASLGLALLLPYINYALFKDYAGNAALVLFVFSIAFTAVIQAYQSIAQSKNSFRPSLKG
                     AGWGLLVKGLTTSFLTGLLGTAGASLSTLLGLGVTLWYFVRIETKELNAFWKERHFGR
                     KLMFSLLCMTVVLLLYYGVLNIVFGPVTHRSTAFFVSVIGVGIGASVFIYAVIRLRLF
                     TVREWLMLPFGKKILRLNKKIKK"
     gene            complement(11455..14976)
                     /gene="mfd"
                     /locus_tag="ACG842_001585"
     CDS             complement(11455..14976)
                     /gene="mfd"
                     /locus_tag="ACG842_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HFQ0248132.1"
                     /translation="MDIIQLVGKDPQVKEWVSNLEEKMPRQLITGLAGSAKTLLFARA
                     FKKTNKNMIILMPNLYYANQLAEDLQHVIPQDQIHLFPVDEVLSAEMAFSSPEARGER
                     VATLNALQEKKAGIYLLPVASLHKRLPNKKTWLQAQLNWHIGDEIDVENLPRQLTLMG
                     YVREEMVAKPGEFSIRGSIIDIYPLTTTYPVRVELFDVEIDSMRYFEAETQRSIEKTE
                     EIWVSPTSEQVYGPEDLEHGIEQMQKMLEKRLAATTEEADREFLQDYFGQLTSSWQAG
                     VPTEEAKFYSDLLYQESVTILDYFPSNSLLVVDDYQRIMETNREIEREAAEWQTQKIS
                     ELRVFSEQTFSADIQKIVQKEKFTTTFFSLFQKGMGNLRFQAIHNFQYRTMQQFFGQM
                     PLLKTEMDRWRKQKQTVVIFIPTKDRIRKAEQMLRDEDILVVETEKNALIRGQVQLVE
                     GALQAGFELPQEKIVAITEKEIFQKTTKKQTRRQTVTNAERLKSYNELKAGDYVVHAN
                     HGIGKYIGMETLEVDGVHQDYMTILYQNDDKLFIPVTQLNLIQKYVASEAKAPRINKL
                     GGSEWTKTKRKVSSKIEDIADDLIKLYAARESEKGYAFGPDDAYQKEFENAFPYSETD
                     DQLRSAAEIKRDMEKEKPMDRLLVGDVGYGKTEVALRAAFKAIKESKQVAFLVPTTIL
                     AQQHYETMLERFEGFPVNIGLLSRFRTRKQQKETIEQLRTGQVDIVVGTHRILSKDIE
                     FSDLGLLIIDEEQRFGVKHKERLKQLRAQVDVLTLTATPIPRTLHMSMLGVRDLSVIE
                     TPPANRYPIQTYVMEKNPGAIREAIHREMGRGGQVFYLYNRVETIEQKVEEIQELVPE
                     ARIGYAHGQMTEAQLENTLFEFIEGQYDVLVTTTIIETGVDIPNANTLFVENADYMGL
                     STLYQLRGRVGRSSRVAYAYFMYEQQKILNEVSEKRLQAIKDFTELGSGFKIAMRDLS
                     IRGAGNLLGAQQHGFIDAVGFDMYSQMLSEAVSRKQGKNSQVEKTTVEIDLGVDAYLP
                     ETYVADQRQKIEIYKRIRELDSQEMLDELEDDLLDRFGEYPEEVAHLLTIGQIKMDGD
                     RALLETIRKQEAHIVFTLSKVGTKRYSVEQLFEALTATKLKASLGVEKEQMVIRLTIP
                     NQMEEAVWLQEIQKFVKALREQKYLSA"
     gene            complement(14993..15553)
                     /gene="pth"
                     /locus_tag="ACG842_001586"
     CDS             complement(14993..15553)
                     /gene="pth"
                     /locus_tag="ACG842_001586"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HFQ0248133.1"
                     /translation="MKMIVGLGNPGTKYQYTKHNIGFMVVDKIAREHQATFKKNPFEA
                     EVAEFFHNGEKILLVKPQTFMNESGRAVGPLMTYFGIYPEELVVIYDDLDLAVGKIRL
                     RQKGSAGGHNGIKSIISHLNTNVFDRIKVGIGRPEGKKTVVQHVLSPFSKENQPLIEE
                     SMCQSVKAVEYLIEGHSFVDAMNRFN"
     gene            15906..16880
                     /locus_tag="ACG842_001587"
     CDS             15906..16880
                     /locus_tag="ACG842_001587"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004459 - L-lactate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HFQ0248134.1"
                     /translation="MTAAAEKKDHQKVILVGDGAVGSSYAFALVTQNIAQEVGIVDIN
                     TAKTEGDAIDLSHALAFTSPKKIYSATYADAHDADLVVITAGAPQKPGETRLDLVNKN
                     LKINRDVVTQIVESGFNGIFLVAANPVDILTYSTWKFSGFPKERVIGSGTSLDSARFR
                     QALAELVDVDARNVHAYILGEHGDSEFPVWSHANVAGLQIYEWVKNNPEIDEEAMVNL
                     FFGVRDAAYTIIEKKGATFYGIAVALARITRAILDDENAVLPLSVFMNGEYGLNDIYI
                     GAPAVINRQGIQKVIEIPLSDSEQDRMAASAKQLKEILDEAFAKLDAE"
     gene            17050..18450
                     /locus_tag="ACG842_001588"
     CDS             17050..18450
                     /locus_tag="ACG842_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HFQ0248135.1"
                     /translation="MTRSSHRKKSKHVALLSVLGVLLVLISFYTYRSTYYTKHFLPNT
                     EINGINVSNLTVEKANEKLKEAYSDKKLAIKEDGKVWQEVPKSELGYKDDFTSELSPI
                     LNEQNGWTWGMTYVSAAEKQEIDPVSQNHQKLDTTVQTLTTKLTDLNKDRTPTTDATI
                     EKAGDSFVIKPEVNGNTIDVDAAVKQLKSAVNSGKDTIELTEFKEKPKVTSEDSSLKE
                     QLASMNAIANVKATYSINGETFQIPSSDIMSWLTYNDGKVDLDTEQVRQYVTDLGTKY
                     NTSTNDTKFKSTKRGEVTVPVGTYSWTIQTDSETEALKKAILAGQDFTRSPIVQGGTT
                     ADHPLIEDTYIEVDLENQHMWYYKDGKVALETDIVSGKPTTPTPAGVFYVWNKEEDAT
                     LKGTNDDGTPYESPVNYWMPIDWTGVGIHDSDWQPEYGGDLWKTRGSHGCINTPPSVM
                     KELFGMVEKGTPVLVF"
     gene            complement(18528..19250)
                     /locus_tag="ACG842_001589"
     CDS             complement(18528..19250)
                     /locus_tag="ACG842_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFQ0248136.1"
                     /translation="MSGHSKWHNIQGRKNAQDAKRGKVFQKLSREIYMAAKAGGPDPS
                     MNPALRLVVDKAKAANMPNDNVERAIKKATSAVDETNYDEITYEGYGPGGVGILVHAL
                     TDNRNRTATNVRVAFTRNGGSLGETGSVSYLFDRKGYIAIERDGLTVDEETMFEDVLE
                     AGAEDLQTSPEVFEIYTAPEDFAAVRDELEKEFTLAQAELTMIPQTTIDLNEEQKEQL
                     ERLVDKLEDDDDVSEVFTAAEM"
     gene            complement(19329..19835)
                     /locus_tag="ACG842_001590"
     CDS             complement(19329..19835)
                     /locus_tag="ACG842_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HFQ0248137.1"
                     /translation="MNSSKKQMKNGNIYLVIALIVVIILFYSSSQTYGQQSQVSRLES
                     WLPNQPFKDTLAGIRFNYGGSMVSINHLGYAKFLEFFLRKGAHFGTYFILGGSLFLGV
                     FPKLKIWWLTAILAWLSATGYAGLDEFHQMMTGGRTPMFQDVMLDSIGALTAVILCVL
                     FAAFKKRL"
     gene            19958..21757
                     /locus_tag="ACG842_001591"
     CDS             19958..21757
                     /locus_tag="ACG842_001591"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HFQ0248138.1"
                     /translation="MSYSLNWNLDSIFSGGSHSDALNQRMKQLEDQMNEYYHRVTKWS
                     PSSDNAEQLNAILQLQETITNGFTQCSSYITALLSANVNDSDAKVLSGKLYAMLPRLQ
                     SAETVLSKKFAEISDNDWNQMLSQSSFETIAFRLNEIRRDGSQLLSEAEENIINTLSL
                     DGLNAWSSHYDTIVASISIPFEQDGQVVELSAGQAFNKMMGDPDPKVRETLFAAWEEA
                     WKEKAPLFTDTLNHLDGFRLSDYELHGVTDFLQKPLEYNRLKKETLSVMWDTIQKNKQ
                     PLVNYLTRKANLFGKEKMEWQDQDAPIILGDLKEKTFSFDEAAAFIIENFNKFSPKMA
                     KFAQSAFEKSWIEAEDRPGKRPGGYCTELPETKESRIFMTYSNSVNEVATLAHELGHA
                     FHSSTMWDLPSLNREYAMNVAETASTFAELIVADATLKVAKTKEEKINLLDTKLQNAL
                     AMFMNIHSRFIFENRFYEARQEGLVSEDKITEMMVEAQKEGYQGALATYHPYFWAAKL
                     HFFIDDVPFYNFPYTFGYLFSLGIYAYANQKGANFEEEYIHLLRDTASMTTEDLAKKH
                     LNVDLTQPDFWQAGIDMVTEDIQTFMELTEEFI"
     gene            complement(21825..>22351)
                     /locus_tag="ACG842_001592"
     CDS             complement(21825..>22351)
                     /locus_tag="ACG842_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFQ0248139.1"
                     /translation="PESSTTEESTTPAPTTPSTDQSVDTGNGTGSSTPAPTPTPTPEQ
                     PKPVTPAPAPSGSVNGAAIVAEAYKYIGTPYVWGGKDPSGFDCSGFTRYVYMQVTGRD
                     IGGWTVPQESAGTKISVSQAKAGDLLFWGSQGGTYHVAIALGGGQYIHAPQPGESVKV
                     GSVQWFAPDFAVSM"
BASE COUNT         6181 a         4874 c         4037 g         7259 t
ORIGIN      
        1 gagtgtttcc cgattcattt ttggctgccg tagcaacttt tatatcatat cagagagaca
       61 agcgattgtc aactcttttt taaaagtttt tttcgatttc gtttttcgaa atctttcggc
      121 aactcattta tcataactta tcgtttgtta tttgtcaaga acttttttga agtttttttc
      181 gatagaatca agcttcgcaa gtggttcttt tttaacgact cagttatctt atcatgtcgt
      241 tagtaacatg tcaataactt ttttgttatg tttttgaata aatattttct tctgtctttt
      301 ggacaagtaa taatatacca actaaattta gcttggtcaa cgaatttttt gtattttttc
      361 aagttttttt caataaacga ttattcataa cttttctttc tttatcgttc gttttcgtct
      421 tctttaatgt ctagaatagt cggcattagc ttttgaactt tcgttattat ttattccttt
      481 ctacacagca aaagctgcct aaggaattcc ttagacagct tttattcgat tatctcattg
      541 ttgggaatag caaaacatcc cgaattgatt gtgcatcagt tagcaacatt actaatctgt
      601 cgatcccgat gcctagacca ccagttggcg gcataccgta ttctaaggct tccaagaagt
      661 cttcgtctac accgtgagct tcgtcatttc ctagttcacg ttctttttct tgcgcttcga
      721 atctttctct ttgatcaatt ggatcgttca gttctgtaaa ggcattagca aattcgcgcc
      781 ccactatgaa tagttcgaat cgatctgtga atcgaggatc ttctggattt ttcttcgcta
      841 aaggcgaaac ttcgactgga tgtccgtata caaacgtcgg ttgtgtcaat gtttcttcaa
      901 caaatgtttc aaagaattca ttgataacat gtccgacacc catagcttcg gttacttcta
      961 cattgtgttt ttcggctaaa gctaatgctt cttccaatgt catttcttgc cagaagtcta
     1021 caccagtttg ttctttgatt gcatctacca tatggatgcg tttaaagtca ctttctaagt
     1081 cgacagcttg tccatcatat gtgatttttg ctgttcctag tactttttca gctgcgttac
     1141 ggataatacc ttctgtcaga ttcattacat cattgaaatc agtatatgct gtgtatgctt
     1201 ctaacatcgt aaattctgga ttatgtgtag tatcaattcc ttcattacgg aaaacgcggc
     1261 cgatttcata aactttttcc attccgccaa caattagtcg ttttaaatga agttctagcg
     1321 caatacgcaa atataaatca atgtccagtg cattatgatg cgtgataaat gggcgtgcgg
     1381 cagctccacc tgcctcattg tgcaatactg gtgtttctac ttcaatatag ccattgccat
     1441 ctaaatagcg acggatttcg ctgataattt ggcttcgttt cataaatcgg tcaaagcttt
     1501 cgcgattact gattaaatcc aagtagcgtt gtctgtagcg ttgttcaata ttcgttaagc
     1561 catggtattt atctggcagt ggacgaagtg cttttgacaa aatcgtgatt tctgaagctt
     1621 tgatagaaac ttcgcctgtg tctgttttca tgatttgtcc agttacacca aagaaatctc
     1681 caaggtctgc atgtttgaat acttcatagg cctcatcacc tacttggtct tttcttacat
     1741 aaatttgaat ttggccttca cgatcttgta gatgcgcaaa tccagctttc ccttttcctc
     1801 ggcgagtcat catacgacca gctacactag cggtcaatcc catttctgct agttcttctt
     1861 ttgttcttgg atcaaatagt tcatgcaatt cttctgagtt gtgcgtgcgt tcaaatcgtt
     1921 ttccaaatgg atcgattcca tcttcacgca attgttccat tttttgacga cgtacaagca
     1981 tttgatcatt taaatcttct acttgatgtt gttgttcctg tgtcacaaaa gttcctccat
     2041 tctcatgtta atctctttcc tataatgcca atgaaacaca aaaaaagcaa gccgaacttg
     2101 gaatttcggc ttgaaaattc acgaaaaact tagaaatcac aacgtttgtt tcaatagtac
     2161 tttttccgct ttttctacaa attgatccag caggtcattg atttcttgct gtttttctgt
     2221 ctgattgatt gctactttca ctttcgctgc tcgaggaatc ccttttaaat aataagccgc
     2281 atgtgttctg aattctctac aagctacttt ttctcctttg agatctgcta agcgttgtaa
     2341 atgaagttta gctgtagcaa ttttttcggc tggtgttggt tcaggaatca gttcccctgt
     2401 ttcgagataa tgctgtgtgc ggtgaatcat ccatggattg cccagcgctg cacgaccaat
     2461 catcacaccg tctgcaccga catagtcaag catccatttt gcatcttctg gcgttttgac
     2521 atccccgttc cccataaatg gaatcgtcaa atgttttttc acttctttca atacttccca
     2581 gtccgctttt ccttcataca tctgaacccg tgtacgtcca tgcatagcga tggcacttgc
     2641 tccagctgat tgtgctgcta atgcattttc tacagcaaag acgtgctctt catcccaacc
     2701 aatacgcatc ttgattgtta ctgggatatt tactgctgaa gaaacagctt ggaccatttc
     2761 gtatactttc tcaggatcta aaagccattt tgctcctgct tctgctttga ttactttatt
     2821 tactggacag cccatattga tatctattat atcggcagct gtgttttctt cgacaaattg
     2881 agcagcttca actagactat ctttatttcc gccaaatatc tgtaaactta atggatgttc
     2941 tgtttcttca atgtggagca tctctaaagt ttttttgttt ctgaaatgaa taccttggtc
     3001 actgatcatt tcacatacga ctaagcccgc accaaattct ttgacagtga ctcgaaatgc
     3061 tgaatttgtt attccagcca tcggtgcaac aacgacacga ttagggattt ccacattgcc
     3121 aattttccat gtgtgttcca cttaaatccc tcctaaggct tctttcttca atccatctaa
     3181 ttcttcttcg ctatacttgt atttgttatt acagaaagaa caaaccgctt ctgcaccgtg
     3241 atcttcgtct atcattgctt ggatttcttc tgcacctaac gcgataattg cttctgcaaa
     3301 tttttctttt gaacagtcac atttgaattg aacaggcatt ttttccaaaa tgttcaattc
     3361 ttctgtacca agcaatcctt tcaatatttc ttctggtgtc tgtccttcat ctaagagggt
     3421 agagattctt ggtgtcgttt ttaattgttc ttcgatcttg ctgattgttt cttcatcggc
     3481 acctggcatg atttggatca tgaatcctcc cgccgtcttg atgctgtcgt cagtattgac
     3541 caatacactc agtccaacag ctgaagggat ttgttctgag atagcaagat agtaagtaaa
     3601 atcttcagcg atttctccag aaacaatcgg cgtttgccca gaaaaaggtt cttttaatcc
     3661 taagtctttt gtgacagtaa acattccttg tgttccaact gctccccgca catcgatttt
     3721 accttcacta tttagaggaa gactgagatg cggatttttg atgtaacctt tcacatctcc
     3781 ctttccattt gcatctacta caattcctgt tgctggacca tcgccttgga ttcgaacagt
     3841 tagtttgtct tctcctttga gcgtcgcacc aagtagcaga ctaccgacca atgtccgtcc
     3901 caaagcagct gaggaggtgt gccaagtatc atgacgtctt tgtgcctccg ctacggtatt
     3961 tgttgcttga acggcgtatg ctcggacgaa tccgtcgtga gcaagtgcct taaccaaata
     4021 atctttcatt tgttatgtac tcctttttcc gtttttaaac tcatagtgaa tcatactagc
     4081 aactactgct tttttcaact ttggaatgct tcttttaaaa ataattcaga atcatctaga
     4141 aaacaggaca tcaccctatt tacaacttaa ataaagaaat ctcccgaaaa ccggtttttg
     4201 ttccggtttt cgggagtcat atcagatcaa ttgacctata tttttttatt tgtattgatc
     4261 gtctgaatga ttatcttctg attgttgaga atctgaatca tgttctgctt tattttcctc
     4321 attcagttca tgttttgctt cttcaaaatc acgtttttct tccgcttgtt tttcagcgtc
     4381 tttttcttcc aaagcacgtt ttgcttcttc aaaagttttc gcttcttttt cgcttggata
     4441 atcatttgac tcagtacctt caggcatcac accgtgttca aacaatgact tgattgcttt
     4501 ggcatccaat gtttcgaatt ccaaaagttt ttcagcaatc agtttatgct gtgcacgatg
     4561 agcttcgatg atttctcgag ctttatcatg agcttccatc aagatttttc gtacttcttg
     4621 atcaatctcg aaagcgactt gttccgagta agcttttgtt tgaccatagt cgcggccaac
     4681 aaacacttga tggtttcctt catattgaac aggacctaaa cgatcactca ttccatattc
     4741 tgttaccatg cttcgtgcta gagctgtcgc ttgctcaaag tcattggaag caccagttga
     4801 ttggacattg aagatgattt cttcagctgt acgtccacca agtaagccta cgatttgttc
     4861 aaacatatct tctttcgtca tcaagaactg atcttctttt ggtaaagcga tcatatatcc
     4921 gccggcgcgt ccgcgaggga tgatcgttac tttgtggacg acccgagcgc gactcaatac
     4981 taatccgaca atcgtatgac cagcttcgtg ataagcaacc atttcgcgtt cttttttgct
     5041 gattacgcga tcttttttag ctggacctgc aatcacgcga tcttccgctt catcgatatc
     5101 agaagcatcg attttctttt tatttcttct agcagctact aacgctgctt cattcaagac
     5161 attttctaaa tctgctccgg caaaacctgg tgtctgttga gccacaactt tcaagtcaac
     5221 gtcatctgcc atcggtttat tccgtgcatg gactcgcaag attgcttcac gaccttttac
     5281 atctggacgt ccaactaaaa tctggcggtc aaaacggcct ggacgtagta acgctgggtc
     5341 tagtacatca gaacggttgg ttgcagcaat gacgattact ccttcatttc cgtcgaagcc
     5401 atccatttct acaagcaatt ggttcaatgt ttgttcgcgt tcatcatgtc cgccgcccat
     5461 accggcacca cgttgacgac ctaccgcatc aatttcatca atgaaaatga tagctggtgc
     5521 gtttttcttc gctgtttcaa acaaatcacg aacacgactt gctccaacac cgacaaacat
     5581 ttctacgaaa tcagaacccg agatcgagta aaacggtact ccagcttctc cggcaacagc
     5641 ttttgctaat aaggttttac ctgttcctgg aggtccttct aaaagtacac ctgccgggat
     5701 acgggcacct aattcaacaa atcggcgagg atctttcaga aattcaacaa cttcaactag
     5761 ttcttgtttt tcttcttccg cacccgctac atcagaaaaa cgtacgcgat tagctttttt
     5821 gtctgcttct ttggcttttg atttaccaaa gttcatgaca cggccgccac cgccaccgcc
     5881 gccgccttgt tgtcccatca tcatgtaaaa gaaaaagatg atgattacga ttggcaagaa
     5941 gctaagcaat agggaaaccc atataccgct gcttgattct tcttttactg ttgtagcaac
     6001 tttgttttct tttgccagtt ctgttacttg gctcaatgtt gtatcatttg gcaaaatgat
     6061 tgttgtgaaa tgggtagtag tcgtatctgt tgaacctaag atagctaacc caccgctgtt
     6121 agcgacggtc tgctgttctt tgtattcacc agtgatctta tatacaccat ttgcaggctg
     6181 cacttcaaat tctttgatct tgcctgcttg tagttgttct gtgaattttg agtactcgat
     6241 atctgacgat cgctggccgt tattaccaaa aatgaaataa acgaccatta ccatcgctaa
     6301 gattacgagg acataataca aggcattttt catcatgccg ttgttctttt tgttcatagt
     6361 tgccctccct aagcgtcaat ttaatttaac agttttttat caatcctgac cattctattg
     6421 taccataatt taattgagat acagcattaa tttgattgat aaacactagg tttcaataca
     6481 ccgatgtacg gaaggttacg atatgcttct gcataatcca agccataacc gaccacaaat
     6541 tcatttggta cgttgaagcc aacataatct gcttcgatat ttactactct tccttcaggt
     6601 ttgtcaagta gtgtaacaat tttgactgat tttgctttgc ggtatttgaa aagatccact
     6661 aaataagcaa gtgttcttcc gctgtcaatg atatcttcaa cgatcaacag atcgcgacct
     6721 tctacattcg tatctaagtc tttgacgatt tttacttccc ctgacgagac tgttgcattc
     6781 ccgtagcttg aaacatccat gaaatccaat tccaaatacg tatcgataga acgaacgata
     6841 tctgccatga atggtaccgc tccttttaag acacctacta ctaggggatt ggtgttttgg
     6901 taaatttctg ttaactcttt gcctagttct ttgcaacgaa cttgtatctc ttcttgtgag
     6961 atcaaaattc tctcaatatc cttttccaac atcaggttct ccttccaatt gcttcttttt
     7021 gatatttgta aagaagtatg tagtgtattt tatcagtttc ttcacgaata ctcaaatatg
     7081 aaaaggcaaa aggtatcaag ctatatatat ttcttttggc atctgtaatg atccaagcat
     7141 gctgcctttg tgaattaggt actttctgat cgataaaata tctagatact ttttttgtga
     7201 gattctctgt taatttaatg cggtctcctg cttttctttt ttcgacatac agttgcacat
     7261 cagcaggaat ccaaatgtca tgagaaaata gagaccatga atctgttgct ttgatgtccc
     7321 ctgcattttc aggtttgacc agaccaatcc attctgtttc attcaaatat attccttgtt
     7381 ctggaatcag caacaagcgc tggtcttctg tctggctagt tgcttgtttt tcttttttag
     7441 agagtacaac aaaatgatac tctcttttca gtatccaatt attgccaata ctgagttgcc
     7501 attggctttt tttctgttgc cactggtcta aaatcgactc catttgttgt tctttcaaaa
     7561 taattcctgt ttttgagaat gctttgtcga aaaaggagta aaaaaaatag tatcgttcag
     7621 catctgtcat ttcttcaatc atttctgcag aaaatcgaaa ttgttcctcc cactgttcga
     7681 cttccttttc gatcaactgt cccatatatt tttgaatgac ttggtctgcc cattggatct
     7741 gttgcgaaaa ttgctggaaa tgtctcaacg cctgtgtatt ttcttgtttc aattgaggaa
     7801 ctaccaaatg acgaagccga tttctctgga tatctaacaa ttggtttgtc tgatcctcaa
     7861 agtaaacaag ctgcgattcc gctgcataag tatataggtc ttctttgcta aaactcagca
     7921 acggacgaat cagtctgccc gtagcaaatg gttgtacttc tttgattcca gaaaaagtat
     7981 ttaactgtcc cccacgaacg attttcatta acaccgtctc gatttgatca tctccgtgat
     8041 gagcagtcat caacgtgtca tattgtcctt cttccatcac gcgagtgaat tcttgataac
     8101 gaaagttacg agcttctgtt tctatggcgg atttggctgg atcttcccaa atacttacat
     8161 ataaagggag attcttctct tcacaatatg agcgaagata ggcagcttct tctgctgatt
     8221 cttctcttaa ttggtgattg atgtggacga tacctaaaga aaaaccgatt tggcgttgca
     8281 cttgtaaaag taaatggagc aaaaccatcg aatcaacacc acctgatacc gctgctaaaa
     8341 ttctcgatcc ttgttgccag aatccttgtt cattcccgtg ccgaataaat gactgctgta
     8401 atagcggtgc ttttttcccg cctttttcaa cgttcatcct ttccacaccc tttcttccta
     8461 aaaaaaagag agcgggaaaa cgtctaaaac gacaattaac ctgccctctg ttgatactat
     8521 gacccaaata gtcgcactat cattatttgc ggctttttag ctgcgacgtc cgccacggcc
     8581 accgcgcttt ccttctgtgt ttcgcttcaa tgaagataag cggtcatcgc tatctttcaa
     8641 aaatgaactc atcaatgaat cgaagtcttc tttttgagct gaaggttgac tgcgagaaaa
     8701 agattttttc tgtgctggac gatttcggtt gtcacgggga tctttttgtt gatatttttt
     8761 aggacggctt tcttctccag attcagcagg ttgttcctgc gctttccgga tagatagtcc
     8821 gatttttcca tcatcattta ctgaaataac tttaactgtt acttcatctc ctacacttaa
     8881 aacatcatgg atgtctttta catagccatt tgacacttcg ctaatatgaa ctagacccgt
     8941 ttttccagca cctagatcaa taaacgcacc aaaatttgtg attcctgata cttttccttg
     9001 cagttttgct cctacctcga ttgacataaa aaaaatgttc ctcctaatta tttccctttt
     9061 ttatattcct gcttattttg cagttgtaga actagttgat tgatctgttt catttgtcga
     9121 gctgtctgta tttgattcta atataggata aactttttca ccgtcttttg agtaaaagaa
     9181 tttgcttctg gcaagtttag ctacatactc atcatctttc agcaatgaaa catcttggtg
     9241 cagctgatca acgtctgcgt tgacttcttt catttcagta tttgcttcga ctctcaattc
     9301 gttcaattga tgcatccttt gcatgtcatg gaaaatctgc acacctatta tcgcgaaaat
     9361 gaccaaagca ccaacaaaaa ttgcagccag tctgcgtctt cgaaaaatca gttgacgttg
     9421 ttgttgctga aattcagcat actttttctt tgcatattcc gtatctagcg cagcgatttt
     9481 attgcttttt ttcgtcactt ggattcgctc caatcttttt gattgtcttt ttcattatac
     9541 caatatcgtg aaagcttgtc taatctattt ctctagtgtt tctgtgcgat tttcagaaat
     9601 aatttcgtac attttttgtg catcttcttt tttggttgat tcatgtaatt ccgtaatttt
     9661 cacttccaac gttttgttgc caaactggac tttcaaaata tcgcctactt tgacatcact
     9721 ggaagatttc gccaatttac cgttgatttg aattcttcct ttatcagcta cttcttttgc
     9781 tactgatcgt cttttgatga ttcgggaaat ttttaaaaat ttatctaatc gcatatctta
     9841 ttcctcactt tttaattttt ttatttaatc gcaggatttt cttaccaaaa ggcaacatta
     9901 gccattctcg tactgtaaac agacgaagac gaatcactgc ataaatgaac acagaagccc
     9961 cgatcccaac tcctatcacg ctgacaaaaa aagcagtgga acgatgagtt acaggaccaa
    10021 atactatatt caatacacca taatacagca gcagtactac tgtcatacaa agaaggctga
    10081 acatcaactt ccgtccaaaa tgccgttctt tccaaaaagc atttagctct tttgtttcaa
    10141 ttcgaacaaa ataccaaagc gtcacaccta atcctaaaag ggtagacagg cttgcacctg
    10201 ctgttcctaa taaaccggtc agaaaagaag tagtcaatcc ttttaccaat agtccccatc
    10261 ctgcgccttt taaagaaggg cgaaacgagt tcttgctttg agcaatactt tgataagctt
    10321 ggatgactgc agtgaaagca atggaaaaaa caaataatac aagcgctgca tttcccgcat
    10381 aatctttaaa caacgcataa ttgatataag ggagcagcaa ggctaatccc aaagacgcag
    10441 ctaatgctaa agcggtcgtc aatctaagat agattttggc tgtctgtaaa aactgacgat
    10501 agatccggtt ggtcaaatga cgagtcaacg caggcaggaa tgtcgcactt aatgcagtgg
    10561 caatcactaa acctaattgt actaaaggct gacctcgatc atagacccct tttgctatct
    10621 tcgcaccgta ttctgacagt ccgtaaactt ccaaagcatt ttttacaaag aaagagtcga
    10681 tcagctggaa aaagataagc aatccactat aaatcgaaac cagtccgcct tctaccagaa
    10741 aacgccggat caagggcttt gacggctgtt tgaacaatgg aaaccgtcgg aagcttaacg
    10801 ctcctccatg aatcttttgt tcatagtagt gaaggatccc atatgcgcat aaaccgccaa
    10861 aaacagctcc actcattgct gccgtccctg tttggtacac cgtccatcca agctttctaa
    10921 aagcaaacgc actacagaca atcacaaaaa cacgaacgaa ctgctcaact acttgagaaa
    10981 cagcagttgg ttccatcaag aatcttcctt ggaaattccc tcgataaaaa gatagaccag
    11041 gcatcagcaa aaaggtaaag gaaactactt ggatcagcgg tttcaattgt atgtctccca
    11101 tcatccaagc aatccagctg ctaaatgcaa agacaaacaa ccacaaaaag acacctgtcc
    11161 aaaatactaa gggataaaga ttttgcaaag cttgctttcg ctcgaaagga ttctttcttt
    11221 ctgcaacgta ctttgatatg aactgcggaa gaccggataa agctaaagtc attgctatcc
    11281 cataaattgg ataaacttgc tgataaacat aaaaaccttc gtcgccgact aagttttgga
    11341 ggggaattcg gtaaagggca cttaatactt ttgctatgaa agaagcaatc gtcaatacaa
    11401 atgctccttg catgacgcgc cgcatttctt tatgatccaa atcattgctc cttcctacgc
    11461 tgacagatat ttttgctcac gcaacgcttt gacaaacttt tggatttcct gaagccatac
    11521 tgcttcttcc atttggttgg ggatcgtcaa gcgtatcacc atctgttctt tttcaacgcc
    11581 tagactggct tttagttttg tcgctgtcag tgcttcgaat aactgttcca cactatagcg
    11641 ttttgttcca actttactta gagtaaagac tatatgcgcc tcctgtttcc ttatcgtttc
    11701 taggagcgct cgatcgccat ccattttgat ctgcccaatt gtcaagagat gtgctacttc
    11761 ttctggatat tctccaaaac ggtcgagcag atcatcttct agttcatcta gcatttcttg
    11821 cgaatcaagt tcacgaattc gtttatagat ctcgattttc tgccgttgat ctgccacgta
    11881 tgtttcaggt agatacgcat cgacaccgag gtcgatctca acagtggtct tctccacttg
    11941 actattcttt ccttgtttac gagaaacagc ttcgcttagc atttgagaat acatatcgaa
    12001 accgactgca tcaataaatc catgctgctg agcacctaag agattacctg caccgcgaat
    12061 ggaaagatcc cgcatcgcaa tcttgaatcc agatcccagt tctgtaaaat ctttgattgc
    12121 ctgaagacgt ttttcactga cttcgttaag gatcttctgc tgctcataca taaagtaagc
    12181 atatgcgaca cggctgcttc ttcctacccg ccctcgcaat tggtataacg tggataatcc
    12241 catataatca gcgttctcaa cgaacaacgt atttgcgttc gggatatcta ctcctgtttc
    12301 aatgatggtt gtagtgacaa gaacatcata ttgaccttca ataaattcaa aaagcgtatt
    12361 ttctaattgc gcctctgtca tttgcccatg agcataaccg atccgtgctt ccggaacgag
    12421 ttcttggatt tcttctactt tttgttcgat cgtttctacc cgattatata aatagaatac
    12481 ttgtccacct cgtcccattt ctcgatggat tgcttcgcga atagccccag gatttttttc
    12541 cataacatac gtttggatcg gataacgatt cgcaggcggt gtttcgatta ctgaaagatc
    12601 ccgcacgcct aacatcgaca tatgcaaagt tctcggaatc ggtgttgcag tcagagtcaa
    12661 aacatccact tgtgcacgta actgtttcaa tcgttcctta tgttttacac caaaacgttg
    12721 ttcttcgtca atgatcagta gacccaaatc gctaaattca atatcttttg acaaaattcg
    12781 gtgggttcct acaacaatat caacttgacc tgtacgcagt tgttcgatgg tttctttttg
    12841 ttgctttctt gttctgaaac gacttaatag cccgatattt acggggaaac cttcaaatct
    12901 ttccagcatc gtttcatagt gctgctgtgc caagatcgtt gttggaacta aaaaggctac
    12961 ttgtttgctt tcctttatcg ctttaaaggc cgctcgaagc gctacttctg ttttcccata
    13021 accaacatca cctacgagta gtcgatccat tggtttttct ttttccatat cccgtttgat
    13081 ctccgctgca cttctcaact ggtcatcagt ttcagagtag gggaaggcat tttcgaattc
    13141 tttttggtag gcgtcatcag gtccaaaagc atatcctttt tctgactctc ttgcggcata
    13201 taatttgatt agatcgtcag cgatatcctc gatttttgaa gagacttttc gcttggtttt
    13261 cgtccattca cttccgccta gcttattgat gcgaggcgct ttggcttcag aggcgacata
    13321 tttttggatc agattcagtt gagtaacagg aataaacagt ttgtcgtcgt tttgatacaa
    13381 aatagtcata tagtcttggt gaacgccatc tacttctagc gtttccatcc caatgtattt
    13441 cccgatacca tgattggcat ggacaacgta atctcctgct tttaactcat tatagctttt
    13501 caatcgctct gcgtttgtta ctgtttgacg tctcgtttgt tttttcgtcg ttttttggaa
    13561 aatttctttt tctgtgatag ctactatttt ttcttgagga agttcaaaac cagcctgcaa
    13621 agctccttca actaattgta cctgtcctct gatcaatgca tttttttctg tttctactac
    13681 taatatgtct tcatctcgaa gcatttgttc tgctttgcgt attctgtcct ttgtcggaat
    13741 aaagatcaca acagtctgtt tttgcttgcg ccaacggtcc atttctgttt tcagcaatgg
    13801 catttgtccg aagaactgtt gcatcgtccg atattgaaaa ttatggattg cttggaaacg
    13861 aagatttccc atcccttttt ggaagagtga gaaaaaggtt gtggtgaact tttccttctg
    13921 cacgattttt tgtatatctg ctgaaaatgt ctgctcagaa aacacccgca attcgctgat
    13981 tttttgagtc tgccattctg ccgcttccct ttcgatttcc cgatttgttt ccatgattcg
    14041 ctgataatca tcaacgacta gtaaactatt tgaagggaaa taatccaaga tcgtcacaga
    14101 ttcttggtaa agaagatctg agtagaattt tgcttcttct gtcgggacac ctgcttgcca
    14161 gcttgaagtc agctgaccga agtaatcttg caaaaactcg cggtctgctt cttctgtggt
    14221 cgcagcaagc cgtttttcta gcattttttg catttgctct attccatgtt ctagatcctc
    14281 tggtccgtag acttgttcac tcgttgggga aacccagatt tcttccgttt tttcgattga
    14341 gcgttgtgtt tctgcttcaa aataccgcat agaatctatc tctacatcaa atagttctac
    14401 acgaacagga taggttgtcg ttaacgggta aatatcgata atgcttcctc gaatactgaa
    14461 ttctcccggt tttgcgacca tctcttctcg tacatagccc atcaaagtca attggcgagg
    14521 aagattttcc acgtctatct catcaccaat atgccaattc aattgcgcct gtagccaagt
    14581 tttcttattc ggcagtcgtt tgtgcaaaga tgcgactggc aacaaataga tacctgcttt
    14641 tttttcttga agtgcgttaa gcgttgcaac tctctcgcct cgcgcttctg gagaagaaaa
    14701 ggccatttca gcagaaagca cttcatccac tggaaataaa tggatctgat cttgcggaat
    14761 cacatgttgc agatcttctg ctaactgatt cgcataatac aaatttggca tcagaataat
    14821 catgttttta tttgttttct taaacgctcg agcaaataga agtgttttcg ctgagcccgc
    14881 taatcctgtg attagttgtc taggcatttt ttcttctaaa ttgctgaccc attcttttac
    14941 ttgcggatct tttccgacta attgaataat atccattcct tttcctcctt cctcaattga
    15001 atcgattcat cgcatccacg aacgaatgcc cttctatgag atattcgact gctttaacag
    15061 attgacacat gctttcttct atcaaaggct gattttcttt gctgaaagga ctaagcacat
    15121 gctggactac tgtttttttc ccttctggcc tgccgattcc caccttgatt cgatcaaaga
    15181 cattcgtatt caaatgggaa ataatacttt tgataccgtt atgcccgccg gcacttccct
    15241 tttgacggag ccggattttt cccactgcca gatccaaatc atcgtagatt acgactaatt
    15301 cctctggata aatcccaaaa taagtcatta gtgggccgac tgcacgacca gattcattca
    15361 tgaatgtttg tggtttgact aaaaggatct tttctccatt atgaaaaaat tcggcaactt
    15421 ctgcttcgaa cggatttttt ttgaaagtag cttgatgctc gcgtgcaatt ttatccacga
    15481 ccataaagcc gatattgtgt tttgtatatt ggtatttggt acctgggttt cctaacccga
    15541 cgatcatttt catttttgtt ttccacctaa ttctaaaaag cagcacaaaa ggctggactg
    15601 ctaattagcg gtccagtcca tcgagctttt ttctttttta cacttgtcaa tgcatagtat
    15661 aacacaaaac aaggcgtttc ggctaaaaga gaatcgttaa taaatgataa agaaattaaa
    15721 aatttcttca aagtttaatg aaataattga tatataaaaa tagtgaaata acgcacatgt
    15781 aagcttttac atataataca accttgttat ctgttatatt ttatagccaa atagcgtgac
    15841 atggggcttg agaaatgtta gcataagcat gggaaaaaac aaatcaatgg aaggattgat
    15901 actaaatgac tgcagctgca gaaaaaaaag atcatcaaaa agttattctt gttggagacg
    15961 gcgccgtagg ttctagctat gcctttgctc tagttactca aaatatcgct caagaagtag
    16021 gtattgtgga tatcaatact gctaaaactg aaggagacgc aattgacttg tctcacgctt
    16081 tggcatttac ttcacctaaa aaaatctatt ctgctactta cgcagacgca catgatgctg
    16141 acttagttgt tattacagct ggcgcaccac aaaaaccagg tgaaactcgt ttagacttag
    16201 taaacaaaaa cctaaaaatc aaccgcgatg ttgtcacaca aatcgttgaa tcaggattta
    16261 acggtatctt tttagttgcc gctaacccag tagatatttt gacttactca acttggaaat
    16321 tctcaggttt ccctaaagaa cgcgtaatcg gttcaggtac ctctcttgac tcagctcgtt
    16381 tccgtcaagc acttgcggaa ttagttgacg ttgatgcacg taacgtccat gcttacatct
    16441 taggtgaaca cggagattca gaattcccag tttggtcaca tgcaaatgtt gccggcttgc
    16501 aaatctatga atgggtaaaa aacaacccag aaatcgatga agaagctatg gttaacctat
    16561 tctttggcgt acgtgacgct gcttacacaa ttatcgagaa aaaaggcgct actttctatg
    16621 gtatcgcagt agcattagca cgtatcactc gtgccatctt agatgatgaa aatgctgttc
    16681 ttcctttatc agtattcatg aatggcgaat atggattgaa cgacatctac attggtgcac
    16741 ctgctgttat caaccgtcaa ggtatccaaa aagtaatcga aattccttta agtgacagcg
    16801 agcaagaccg tatggctgct tctgctaagc aattaaaaga aattctagat gaagcttttg
    16861 ctaaactaga cgctgaataa tctttgaaca tgacggaaaa catctattcg gtgttttccg
    16921 tttttttatt ttatcaccct tatggtttct aaattttttc ataaagtaga gataaccttc
    16981 gtcttttcct ctaaattatg ctatcatgag accgttccac cctaaacttt tacttaagga
    17041 gttgtcgata tgacaagatc ttcgcatcga aaaaaatcga aacatgttgc tttgctttca
    17101 gttcttggtg ttctcttagt gctgatcagc ttttatacct atcgcagtac atactacaca
    17161 aagcattttt tgccgaatac agaaataaat ggaataaatg tcagcaatct aacagtcgaa
    17221 aaagcaaatg aaaaattaaa agaagcttat tctgataaaa aactggctat taaagaagat
    17281 ggaaaagtct ggcaggaagt accaaaatcc gagctcggct acaaagatga tttcacttcg
    17341 gagctttctc cgatcctaaa tgaacaaaac ggatggactt ggggaatgac ttatgtctca
    17401 gcggcagaaa aacaagaaat agatcctgtc agtcaaaacc accaaaaact agacactacc
    17461 gttcagacat tgacaactaa gctgactgat ttgaacaaag accggacacc tactactgat
    17521 gcaacgatcg aaaaagcagg tgactctttc gttatcaagc ctgaagtcaa tggaaatacg
    17581 atcgacgtcg atgctgcggt caaacaatta aaaagtgcgg taaactctgg taaagataca
    17641 attgaattga ctgaattcaa agaaaaacca aaagtaacat ctgaagacag ctcactgaaa
    17701 gaacaactcg catccatgaa tgctatcgca aatgtaaaag ctacttacag tataaacggt
    17761 gagactttcc agatcccatc aagtgatatt atgagttggc tgacctacaa tgatggaaaa
    17821 gtcgatttag atacagaaca agtacgtcaa tatgtaacag atttaggcac aaagtataac
    17881 acaagtacga atgacacaaa atttaaaagt acaaaaagag gagaagttac ggtcccagtt
    17941 ggcacttaca gttggacgat ccaaacagac agtgagacag aagcattgaa aaaagcgatt
    18001 ctggcaggcc aagattttac tcgttcaccc attgtgcaag gtggcacaac cgctgatcat
    18061 ccattgatcg aagatacgta tatcgaagtt gatctggaga accagcatat gtggtattac
    18121 aaagacggaa aagtggccct tgaaacagat atcgtttccg gaaagccaac tacacctaca
    18181 cctgctggag tcttctatgt ttggaataaa gaagaagatg caactttaaa aggaacaaat
    18241 gacgatggaa caccttacga gagtccggta aactactgga tgccgattga ctggacaggt
    18301 gttggtatcc acgattcaga ctggcaacct gaatacggtg gagacttatg gaaaacccgc
    18361 ggttcacacg gctgcatcaa caccccacca agtgtaatga aggaattatt cgggatggtc
    18421 gaaaaaggaa cgcctgtatt ggtcttctaa caaaaataat atataaaaag aaagcacccg
    18481 tacgattggt acaggtgctt tctttttatt atctaacaat gtgctactta catttcagca
    18541 gcagtaaaga cttctgaaac atcatcatcg tcttctagct tatctactag acgttccaat
    18601 tgctcttttt gttcctcatt taaatcaatc gtcgtttgtg gaatcatcgt caattctgct
    18661 tgcgccaaag tgaactcttt ttccaactca tctcgtacag cagcaaaatc ttctggtgca
    18721 gtataaatct cgaatacttc aggagaagtt tgcagatctt ctgcccccgc ctctaataca
    18781 tcctcaaaca tcgtctcttc atcaacagtc aacccatcgc gttcaatagc gatatatcct
    18841 ttgcggtcaa acaagtaact gactgaaccc gtttctccca gtgatccacc gtttcgagta
    18901 aaagctactc ggacatttgt cgctgtccga ttacggttat ctgtcaatgc atggactaag
    18961 attcctactc cacccggacc atatccttca tacgtgattt catcgtaatt cgtttcatct
    19021 acggcactcg tagccttttt aatcgcccgt tccacattgt cgttaggcat atttgcagct
    19081 tttgctttgt ccacaacaag ccgcaaagct ggattcatag atggatcagg accgcctgct
    19141 ttagctgcca tataaatttc acgagaaagt ttctgaaaga ctttccctcg ttttgcatct
    19201 tgggcatttt ttcttccttg gatattatgc catttactat gtcctgacat gatttttcct
    19261 cccttgttct tttgagaata cacttctatt ctagtactat ttcacaaaaa aacaaagcca
    19321 cagccaatct ataaacgctt cttgaatgcc gcaaaaagca cacataatat cacagcagtc
    19381 agagcaccga tcgaatccaa catgacatct tggaacatag gtgttcgtcc accggtcatc
    19441 atctgatgga attcgtccaa acctgcgtac cctgttgctg aaagccaagc aaggatcgct
    19501 gtcagccacc aaatttttag cttggggaaa acacctaaaa acaagctgcc tcccaaaata
    19561 aaataggtac cgaaatgtgc gcccttcctt aaaaagaact ctaaaaattt ggcgtagccc
    19621 agatggttga tactcaccat acttccacca taattaaaac gtatacccgc taatgtgtct
    19681 ttaaatggct gattaggcag ccagctttct aatcgcgaaa cttgtgattg ctgtccgtaa
    19741 gtttgagaag aactgtaaaa taagattata acgactatca gtgcgattac taaataaata
    19801 tttccgtttt tcatttgttt ttttgaagaa ttcataagtc acctctgtat tttatcttag
    19861 actttttttg aagaaacaac aatcattttc atagagactt cttccataaa aaagtgctat
    19921 acttaatttt gtattacaat atgaagggag ttttttcatg agttattcat taaattggaa
    19981 tctagattct attttttccg gaggcagtca ttctgatgct ttgaaccaac ggatgaagca
    20041 attggaagac caaatgaacg agtattatca ccgcgtaaca aaatggtcac ctagttctga
    20101 caatgcagag cagttaaacg ctattcttca attgcaagaa acgattacga atggttttac
    20161 ccaatgcagc agctatatta ctgcattgct ttctgccaat gtcaatgatt cagatgcaaa
    20221 ggtcttatca ggcaagcttt acgcaatgct gcctcgtctg caatcagccg aaactgtatt
    20281 gtcaaagaaa tttgcagaaa tatctgacaa tgattggaat cagatgcttt ctcaatcttc
    20341 gtttgaaaca attgcttttc gtttaaacga gattcgtcgt gatggctcac aattattatc
    20401 agaagctgaa gaaaacatta taaatacgct ttctttagat ggtctaaatg cttggagcag
    20461 tcattatgat acgattgtag ccagcatttc gattcccttc gaacaagatg gacaagtagt
    20521 cgaattatct gctggacaag ccttcaataa gatgatgggc gatccagatc caaaagtcag
    20581 agaaacactt ttcgcagcat gggaagaagc ctggaaagaa aaggcacccc tcttcacgga
    20641 tactttgaat catttagatg gttttagact ttctgactat gaacttcatg gtgtcacaga
    20701 ttttctacaa aaaccattag agtacaatcg gttgaagaaa gaaacattgt ctgtcatgtg
    20761 ggatacaatc cagaaaaaca aacaacccct tgttaattac ttgactagaa aagctaatct
    20821 tttcggtaaa gaaaaaatgg aatggcaaga tcaagatgca cctattattt taggcgattt
    20881 gaaagaaaaa acctttagtt ttgacgaagc agcagcattt attatcgaaa actttaacaa
    20941 attcagtcca aaaatggcta aatttgccca atctgctttt gaaaagagct ggatcgaagc
    21001 agaagatcgt cctgggaaac gtcctggtgg ttattgtact gagttaccag aaacaaaaga
    21061 atcaaggatc tttatgacct acagtaattc tgttaatgag gttgcgacac ttgctcatga
    21121 actaggacat gctttccata gcagcacgat gtgggattta ccttctttaa atcgcgaata
    21181 tgccatgaat gtcgcagaaa cagcaagtac attcgcagaa ctgatcgtag ccgatgctac
    21241 attaaaagtc gcaaaaacaa aagaagaaaa aatcaatttg ctagatacaa aactgcaaaa
    21301 tgctttagct atgttcatga atatccattc ccgattcatt tttgaaaatc gattctatga
    21361 agcacggcaa gaaggattag tctcagaaga taagatcaca gagatgatgg ttgaggcgca
    21421 aaaagaaggc tatcaaggag ctttagctac ttaccatcca tatttctggg ctgcaaaatt
    21481 gcatttcttt attgatgacg taccgtttta taacttccca tacacatttg gctacctctt
    21541 tagtttgggc atctatgctt acgccaacca aaagggagca aattttgaag aagaatatat
    21601 ccatttacta cgcgatactg cctcaatgac gacggaagat cttgcgaaga aacatttgaa
    21661 tgtcgatctt acccaaccag atttttggca agcaggtatc gacatggtga ctgaagatat
    21721 ccaaacgttc atggaattga cagaagaatt tatctgattt ctattttatc acaataaaaa
    21781 aagagttagt ccagaatggg ctaactcttt ttgattaatc attcttacat gctgacagca
    21841 aagtcagggg caaaccattg aactgaacca acttttacgc tttcgcctgg ttgaggagca
    21901 tggatatatt gtcctccgcc taaagcaatc gctacgtggt aagttccgcc ttgtgacccc
    21961 cagaataaca agtctccagc ttttgcttga gaaactgaaa tttttgttcc agctgattct
    22021 tgaggaactg tccaaccacc gatgtcacga ccagttactt gcatgtaaac atagcgtgtg
    22081 aaccctgagc agtcaaagcc acttggatct ttaccgcccc aaacatatgg agtaccaata
    22141 tatttgtaag cttctgctac gattgctgca ccattcacac ttccagaagg agctggagca
    22201 ggtgttacag gttttggttg ttcaggtgtt ggtgttggcg ttggagccgg agtactactt
    22261 cctgtgccgt ttcctgtatc aacactttga tctgttgatg gtgttgtagg cgcaggtgtt
    22321 gttgattctt ctgttgttga agattcaggc a
//
LOCUS       DAZKLM010000047        22301 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3764
            SAMEA115706913-rid22582563.denovo.047, whole genome shotgun
            sequence.
ACCESSION   DAZKLM010000047 DAZKLM010000000
VERSION     DAZKLM010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706913
            Sequence Read Archive: ERR13196647
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22301)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22301)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287823.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287823.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,004
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,849
            CDSs (with protein)               :: 2,849
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 41 of 96
            Pseudo Genes (incomplete)         :: 52 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 15 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22301
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706913-rid22582563.denovo.047"
                     /isolate="VRE3764"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            5..403
                     /locus_tag="ACG842_001593"
     CDS             5..403
                     /locus_tag="ACG842_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HFQ0248140.1"
                     /translation="MTIFETVVTDVGIEAEVFRKEKIMILFGDEAPETLTDYCYKIEL
                     NQVRKEITCDMKLCFDKQMYVITAVGDVVQTNLNELGHITIKFDGSHEPELPGTMYVE
                     DKEMPHLRKGTIIKIDQEDQNGGKLYGNHA"
     gene            387..1082
                     /locus_tag="ACG842_001594"
     CDS             387..1082
                     /locus_tag="ACG842_001594"
                     /EC_number="4.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HFQ0248141.1"
                     /translation="MEIMLDTANTKSIYSCQQFLNLTGVTTNPTILKKEGASNFFSHM
                     KEIKKIIGQIPIHIQTIGRTEEEMIEDARVIIEELGKDTFIKVPVNQAGLQAIKKLKK
                     EGYLITGTAIYTELQGYLAINNGADYIAPYYNRMQNQGIDAMKVIDSLRTEIERTQRP
                     TKILAASFKNCEQVTTAIRSGAHAITLGKELLDQVLEMPSIDKAVRGFQEDWVQTFQA
                     ETIGELKKVADYF"
     gene            complement(1676..2563)
                     /locus_tag="ACG842_001595"
     CDS             complement(1676..2563)
                     /locus_tag="ACG842_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFQ0248142.1"
                     /translation="MGNFLKKQLLIMCLSACAILLFSGCSKSNETIATFKGGTLTVQD
                     FYDNPKVKSNNQTLLKKMIVFKAFDDVYGKEISSKEVTKEYNEQIKKLGPNYKEQLKA
                     VGQTEETYKKLFKQMLAFQYGLKANVKLTDKDLDTAWKEFYPEVSTQIILFSTKEEAD
                     KAKKEANEGENFSKLVQAYGKNKTLKETDGKMNFDSTNPEIPTEVKKAAFKLKNGEVS
                     DIIPVTDPTTYQQSYYLVKMVKKQDKGSNKDKYKSELEKIATEAKTLDTEFMDKTIRK
                     VMKKDNVTIKDPYVKNIFK"
     gene            2748..3587
                     /locus_tag="ACG842_001596"
     CDS             2748..3587
                     /locus_tag="ACG842_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HFQ0248143.1"
                     /translation="MIRIIRLIGSVLIVLVGFVLGILVNNALADMFIGNSEWIGAIAG
                     TVGHLAVFLLALFLASKVEGKRIEDYGVSVRGKDWGYLAGGLVVGIGVFLLITSPLYL
                     TGAYRLDSGNANIVPLITSFILFIAVGASEELLFRGFFQHQMLRFGPLIAMIGSAALF
                     ALLHGLNPNMTFLAVFNIFLAGCFFSALIYSTGSLFTAIGAHITWNWTQGAILGIPVS
                     GTQEQGFFSTLIQSQNTIITGGNFGAEASISTTLFLLVLIVGLLLYAYKTKRMKKYTA
                     VYQ"
     gene            3783..4757
                     /gene="sgrA"
                     /locus_tag="ACG842_001597"
     CDS             3783..4757
                     /gene="sgrA"
                     /locus_tag="ACG842_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored fibrinogen/nidogen-binding
                     adhesin SgrA"
                     /protein_id="HFQ0248144.1"
                     /translation="MKKTATVLFVILGLSIIAAMIGISTSANERANENLSFTVKTDRI
                     VYDMTQQVITIPVKPNKSVNASDVHAVLTYGWDGNGSSEKVIGEVYLKDVQWTAGIEY
                     TIMISAELSIDEIKSKDKVDLIVFYDGQMTITENLKPSSWTVVGPPSSTDSTSSESST
                     ENTSGESSTESTSSESSTENTSSESSTESTSSESSTGSTSSESSTESTSSESSTGSMS
                     SESSTGSTSSESSTGKVNNELDLVPIIPTKEQKRNAGSNLKSVLKQPDFVSRGIDHQE
                     KAKETDNRKSDLPETGSRTLNKLITWMGVLLILIVGASYFRSLFHRVK"
     gene            5055..5411
                     /locus_tag="ACG842_001598"
     CDS             5055..5411
                     /locus_tag="ACG842_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0248145.1"
                     /translation="MFEEFIDINERQVYQFLNYCYERDEKLYVVKDIALDLNYTLAKM
                     NSVIQQAESFCERYPEYKLSFLSENKMIKVEFSSQFLLSKVYSILLEGTIGYILLDSL
                     YKGTYQSLENLSQKII"
     gene            5456..6571
                     /locus_tag="ACG842_001599"
     CDS             5456..6571
                     /locus_tag="ACG842_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0248146.1"
                     /translation="MKNYHLSCSLKKGNPIEGEEYRIRYFFHLMYWQSFDENHSDILS
                     SIKNKRQLLEKFAKHAPYTRSIDRKKWISLLRISLQRMKQGHSITYVPEEMNKFVHPL
                     ITKEEFTHYILEPFFEANFWSMNHITPTEQAYLYFMFGVMNTYLEEDLLDTPDKVQES
                     WGWIESSIVENFEKNFKICFTTSEKNYLYVNLAMIHLYSRVFGTKKKVDAFGKSAEDR
                     DFQHIFPVMYPIMQQFFSGVARRIPELNTYYEKNRRLIFQYCMLVRDIFIKHEQPVIF
                     YIQSKYGKTQELWAKKRILSMTERPIKYSASADQLPDIVISDYPIDKKAYDQTNTQIF
                     YWNGQPTKNDWARLKAEIIKIRNEKNRSILEKLDFVN"
     gene            complement(6597..7037)
                     /locus_tag="ACG842_001600"
     CDS             complement(6597..7037)
                     /locus_tag="ACG842_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HFQ0248147.1"
                     /translation="MMIKLYTGSGCSSSRRAKRWLIVHDIPFIEQNLIRQPLTKNEFI
                     HLLKLTNNGVTEMLSTHSISYKNLVHRIDELSLSELYIEIRTSPSLLKKPIIYDQSAK
                     LLTGFSEDKIRMFLSKTKRKQELNELLLSGHLVDFKENNLFAMS"
     gene            7197..7703
                     /locus_tag="ACG842_001601"
     CDS             7197..7703
                     /locus_tag="ACG842_001601"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase family protein"
                     /protein_id="HFQ0248148.1"
                     /translation="MSNTADAIVVIDLQKGVCFDSSPIHELETVTALVNKRIKTYETI
                     GKPVIFVQHEDAQLVPQTKPWELLSELSIPEHSYFVRKTHANSFYKTNLSVLLTELDC
                     HSIELCGAQAEYCIDTTVKFAHGLGYQLMMCRGATTTYDNPFMKAKETIAFYEKIWDK
                     RFLTFLEQ"
     gene            7871..9784
                     /locus_tag="ACG842_001602"
     CDS             7871..9784
                     /locus_tag="ACG842_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFQ0248149.1"
                     /translation="MNSLRTKQVLKGALVILFAVSTSVPQWTVYGESISSESESREVE
                     TTETTDAGNETKSSTSQTETNTETTVETSVTSTDQETTATTDNQQTEEATATKMSNDE
                     SSSAKVSDKENGTENDDIGKGENFNGKEYLNYFGKQMTEILNGPTEDIKSKLIELLVT
                     ELAVNDYGVDKEIIKHLIATYLLYDIAFAKNSVLMSMLGGTDDGTTPTENTGFYPNTK
                     QLHQTVLNKQDGKMMDVYAYYVDQGSDKTVMIHGGFRGNWNNGIVTEEYNDFYKAGYN
                     LLFVDSRATGNSGGDYVTYGQYESDDVLYWINQEVRERPSQKILLYGGSMGAATMMSV
                     LAKDIPVNVKGIIENCGFASIDEQLRFTYSQTVAPALPDAIKNQLDIIGDQEHEDLFM
                     GLLKQYYFDQEMHLDTKAALPTIGMSDSLPKLIIHGTADDVVPVSNAQKLYELSGGYK
                     DLLLVEGAGHGKAQEVDHAAYTKHVTDFLKVVFHDQINVKYVDENNKSLLKDQDEIQL
                     YGAYGENYMTEQKTFEGYELANVEGPTEGIFNETIPTIIFYYKKIPVAPPKKQDPTEN
                     ADNKGKNEPREDQSSDDTQPIQMTVKVQKKTDAKKRMLPKTGEKKHSLLIYSGGFLSV
                     STIITWIWRRKRG"
     gene            9789..10790
                     /locus_tag="ACG842_001603"
     CDS             9789..10790
                     /locus_tag="ACG842_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFQ0248150.1"
                     /translation="MKWFKRIVIAVPVIIVVILVTVFLINQITPKPISLLVRKQFDTS
                     EKEQVYARPDDFQQKIEQIDIKKEISYPSKYKNNNMDIYTPKVEKKKLPILFWMHGGA
                     YVGGDKNDCRDYLEYLCSDTQQIIVNIDYERSPEAKHPTPVIQLNEAIQAAKKEIKDQ
                     ADWSNISIGGNSAGAQISGEYLLALQNEEIKKADHLDPTLENKQITKFISLSGLLDPS
                     ALTQVSDKISSFLYEKCGWAYFDNKDFEQSEKVKSLALVRHADRWTQNTFLTDGNTNT
                     FTKQMEEVTSVFEKAGVKVTKVDYAEKNAVLNHEYQFDFSNKQAQETYQKLVMFFKE"
     gene            complement(10992..11198)
                     /locus_tag="ACG842_001604"
     CDS             complement(10992..11198)
                     /locus_tag="ACG842_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3955 domain-containing protein"
                     /protein_id="HFQ0248151.1"
                     /translation="MKAIHISLLFFASALILFAIHLCSQPYINNNGILVEPAFFCLPL
                     GFLSLLASAGFGFVAFFKRSKQQI"
     gene            complement(11844..12692)
                     /locus_tag="ACG842_001605"
     CDS             complement(11844..12692)
                     /locus_tag="ACG842_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFQ0248152.1"
                     /translation="MSVSIRLQIKTMYNDFSPKEQAIADYILENPSKVSHSPISNLAS
                     ELGIADSTFFQFTKKLGYNGFKDFKMAMLIQENDLSAISIHENIQKTDTELTMAQKVF
                     ESSISTLNDTKKLLQQKDLKKAATIINESKRVYFFGVGGSEIVATDAYHKFLRSPIAT
                     SHSTDYHIQLMEASLLTEKDCAVLISHTGQSKETIHIAETVRKTGAKTIVITSQANSS
                     LAKLGDVVFISISEETEFRSEALASRISQLSILDSLYVILMFYNEKESRRSIAKVRHT
                     ISEIKE"
     gene            12856..13758
                     /gene="gnd"
                     /locus_tag="ACG842_001606"
     CDS             12856..13758
                     /gene="gnd"
                     /locus_tag="ACG842_001606"
                     /EC_number="1.1.1.343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydrogenase
                     (NAD(+)-dependent, decarboxylating)"
                     /protein_id="HFQ0248153.1"
                     /translation="MEIGVIGLGKMGLNLALNLKEHHHKVQGLDISSTAVSQARENDI
                     PAYQEISEFLATFEQKKIIWLMLPAGEITENMLRKLFPKLGPGDIIIEGGNSYYKDSI
                     RRAEEFQKNEIGYLDCGTSGGIEGARHQACLMIGGDKETFLSVEQLFKDVAIERGYLY
                     AGASGSGHFLKMIHNGIEYGMMQAIGEGFQLVQKSEYAFDLEQVARVWNHGSVIRGWL
                     MEIMEDQLNEHPNLSNYRGIVSASGEAKWTVETALEMAVPVPVIATSLFMRNLSQEED
                     SFSAKVVSALRNGFGGHEIVEKGE"
     gene            13760..14761
                     /locus_tag="ACG842_001607"
     CDS             13760..14761
                     /locus_tag="ACG842_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-isomer specific 2-hydroxyacid dehydrogenase
                     family protein"
                     /protein_id="HFQ0248154.1"
                     /translation="MPDYHIAIVNSSSFGRVFPHHLERLKSVGDVHFFTVDQEIPGKE
                     LAELLKGFNIIIASVTPFFRRDFFENKDELLLISRHGIGYNNIDLKAAKEHQTIVSII
                     PALVERNAVAENNITNLLSMLRRTTQAQARVKDDRWEDRAEFVGRTLFNKTVGVIGAG
                     NTGSGVIEILRNGFRCDVLAYDPYKSDLYIESFGAKKVELQELLEQSDIICLCANLTE
                     ENYHMISDKEVDQMKENVYLSNSARGALVDEEAMINGLKSGKIAGYATDVLEEEPGRQ
                     DHPYLAFEQVVITPHISAYTRECLEAMGEKCVRDVEQISQHLLPERSVQTKSNYIKG"
     gene            14758..15042
                     /locus_tag="ACG842_001608"
     CDS             14758..15042
                     /locus_tag="ACG842_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HFQ0248155.1"
                     /translation="MMKKMVNGLKVKTGPQFYLYEEGGISKVSDLLKSYGAKRVLVTH
                     GTVSWEKALPKLVFLNDETIQFFYHRYSGECSYAEARRIATIIKKMKSIS"
     gene            15075..15872
                     /locus_tag="ACG842_001609"
     CDS             15075..15872
                     /locus_tag="ACG842_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HFQ0248156.1"
                     /translation="MGYSANLARIDFGVIPTLASNCAPWTPLAVMYKENGQAEGKTEH
                     FYRQAAFFITDPQLVIDSPVPYFIAGLADTLAKWYESELILEQDFLQDESFLKLAQDT
                     AKICKEEILNHSAKAIQDMAQRKLTSEFKQLSEIIFAVAGLVGGLGDKYARNVAAHAM
                     HDAISKYIPESHRFLHGEKVAYGIFYQLALEGRWEKIDELYPLYRELELPVSLEQMGL
                     FPKDKQIIDKMAEFIDSKEKVHLIPIEVTQKSLIQSMMELEGYIRKI"
     gene            15886..16719
                     /locus_tag="ACG842_001610"
     CDS             15886..16719
                     /locus_tag="ACG842_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFQ0248157.1"
                     /translation="METLSVVQSLLIALWVAAVMSRWLGGGATLTLRFSPLMTGLVVG
                     LIMGEVAQAMIVTAALQMIYMGVFSPGGSMPAEPSIAAAIAVPVALLGDLSPEAAIAV
                     AVPVGLLGSYLYQFRFFINTFLGKYTDKAVEDLNTKAMARSIILYPTVASFLLFVPLV
                     FIALYLGAPLIADVIKALEGTVVIHILEVVGGGLAAIGIATTVYVIGRKDYLVFFFLA
                     YFMSVVLKSLDITMVTYAIFGIIIALIFVQSQKGKQVQPATSNNNSASFDDEDDYDDG
                     F"
     gene            16737..17594
                     /locus_tag="ACG842_001611"
     CDS             16737..17594
                     /locus_tag="ACG842_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFQ0248158.1"
                     /translation="MAEIMDNQTGAPEEITKKDVTKAYLRWHFANEIPHSFERYLAPS
                     LLYGMMPILKKLYKDEDSLKAAYKRQLLFFNTQLSWGGGVITGLMSSMEQQRAEEEYE
                     EKEILMQDDLMYNTKAGLMGALAGIGDAIDSGTVQYIFIAIAVPWAQQGSPLGALFPF
                     ICFGLYQVLLGVFFARQAFTMGRNATGLKQNTGVQTAIEMLSVLGLFMMGVLAGNYVK
                     VESSLKFAISGREFVIQEILDQIVPGILPLAVVMGVYLFYTKKGLKVTQALLWLTGIL
                     IVLAGIGIL"
     gene            17616..18125
                     /locus_tag="ACG842_001612"
     CDS             17616..18125
                     /locus_tag="ACG842_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HFQ0248159.1"
                     /translation="MGVVNLARVDERLIHGQVMVTLSQKSGVNSIFVVDEVVAKDKFM
                     RDLYKNAGSRTGQKTIVITPEKAKFYWDEYQFKEYNCILIAKTVQTIYDLVKHGIPME
                     ELNIGGIAQKNPDTDLLVTKSVYLNKSDAEKLKEMNEQYGVKNIYFQATPSAPKTGLK
                     DVLNKFDLS"
     gene            18162..18563
                     /locus_tag="ACG842_001613"
     CDS             18162..18563
                     /locus_tag="ACG842_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0248160.1"
                     /translation="MKKNELFRDWEFRYRYIYRKRRTKKSKQRFLSALVSDIYSMRTD
                     VTVIAYDTLAYRSKNIYVGDIEKAEKVICTYYDTPVHALGSYFMFDWKDQRKKTIYSI
                     LLSFILLFSLGWWGMMIYNKNPHHVFDLLSV"
     gene            18675..19028
                     /locus_tag="ACG842_001614"
     CDS             18675..19028
                     /locus_tag="ACG842_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0248161.1"
                     /translation="MLEMIRTIDDPSVAYAFVDEGCYGEKGLDSVRSGMKKEAILFYL
                     DSVGADTPLQFSGNYFSNKEQWLKQVDKLKEKNVNYIFSARKKQAQFFYLTKTDLRGK
                     TFNWQNANQIIALFR"
     gene            19062..19505
                     /locus_tag="ACG842_001615"
     CDS             19062..19505
                     /locus_tag="ACG842_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFQ0248162.1"
                     /translation="MLEIMIVTHGKLSEGLKDAAEVIVGTSSHIETMCLESGEDIQTF
                     GEKVKEKLTMINTAEPMLVFVDLISASPYNQALLAVNELTEEKKAATYVIGGVNLPML
                     LEAINHQMLHTPIEEAVEQIVKQGKDNLTVWHASAEEKKLDEDDF"
     gene            19522..20607
                     /gene="uxuA"
                     /locus_tag="ACG842_001616"
     CDS             19522..20607
                     /gene="uxuA"
                     /locus_tag="ACG842_001616"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HFQ0248163.1"
                     /translation="MKWGFRWYGEKGDSIPLKYVRQIPGINGIIGTLLTKMPGDVWEI
                     SEIEELKASVESQGLELLGIESVAIHDAIKAGTDERDYYIDNYIRTIKNLSRCGVRLI
                     CYSFKPIFGWAKTDLFNENNDGSYSLLYDQKIVDQMEPGEMYQLIHSQSKGFKLPGWE
                     EERLKKFAQLEKLYEGMTEEELFENLTYFLEKVIPVCEKADVKMAIHPDDPPWELFGF
                     PRVTKNLGDLKKILNAVDSPYNGLTLCTGSLGANPENDLVEIIHEVGSRIHFVHFRNV
                     EFLGERKFKETAHPSTEGSLDMYHIMKALVQEGFDGPIRPDHGRTVWGEVAMPGYGLY
                     DRAMGLTYMQGLYEAISKEKPLGEHYE"
     gene            20600..21313
                     /locus_tag="ACG842_001617"
     CDS             20600..21313
                     /locus_tag="ACG842_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFQ0248164.1"
                     /translation="MNKIKLFIFDMDGLLLETGRLAYRAYVKSAQKYDYEMRKEVYYL
                     LTGQTEMAIREQMGILYGEDVPYIKWREAINQYKEKIVKEDKRVYTKKGAEEILSFAK
                     ERGIHTIVASSNTRVKVEMYLKMENLYALFDQIISGDDVKKGKPEPEIFLKACSKMNI
                     PPSEALVFEDSIAGIEAARRAGILSFLIKDHLIGLPDHNGKHKLKVDVSLYESNKADY
                     EFYDLHQAKRFLESKGLYL"
     gene            complement(21520..21669)
                     /locus_tag="ACG842_001618"
     CDS             complement(21520..21669)
                     /locus_tag="ACG842_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0248165.1"
                     /translation="MTFYNKDIQYRVTLDTDLNLFVVFDKENTNHVATGVTIEQAVQE
                     LKKSA"
     gene            22065..22265
                     /locus_tag="ACG842_001619"
     CDS             22065..22265
                     /locus_tag="ACG842_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFQ0248166.1"
                     /translation="MNNGTVKWFNADKGFGFITGEDGNDVFAHFSAIQGDGFKTLEEG
                     QAVSYDVEDGQRGPQATNIVKA"
BASE COUNT         7683 a         3427 c         4620 g         6571 t
ORIGIN      
        1 agagatgacg atctttgaaa cggttgtaac agatgttggt atagaagcag aagtattcag
       61 gaaagaaaaa ataatgattt tatttggaga tgaagcaccg gaaacattaa cagattattg
      121 ttataagatt gaattgaatc aggtaagaaa agaaataact tgtgatatga agctctgttt
      181 tgataaacaa atgtatgtaa tcacagcagt aggagatgtt gtccaaacta atttgaatga
      241 attaggacat atcacgataa aatttgatgg atctcatgag ccggaacttc caggaacaat
      301 gtatgtagaa gataaggaaa tgccgcattt aagaaagggg acaatcatta aaatcgatca
      361 agaggatcag aatgggggaa agctatatgg aaatcatgct tgatacagca aatacaaaaa
      421 gtatttattc ttgtcaacag tttttgaatt taacaggcgt aacaacaaat cctacaattc
      481 tgaaaaaaga aggagcgtca aacttcttct cgcatatgaa agaaatcaaa aaaataatcg
      541 gacagattcc tatccatatt cagacaatag gaagaacgga agaagaaatg atagaggatg
      601 ctcgagttat catagaagaa ctgggaaaag atacgtttat caaggtgccg gtgaatcaag
      661 caggccttca agcgatcaaa aagttgaaaa aagaaggtta tttgattacc ggtacagcta
      721 tttacacaga attacaaggg tatttagcta ttaataatgg agcagattat attgcacctt
      781 attataacag gatgcaaaat caaggcattg atgctatgaa agtgattgat tcgttgcgga
      841 ctgagatcga acgtacgcaa agaccaacaa aaatattagc agctagtttc aaaaattgtg
      901 aacaagtcac tacagccatt cgatctggag ctcatgcgat aacgttaggt aaagagctac
      961 tagatcaagt attagagatg ccctcgattg ataaagcagt gaggggcttc caagaagatt
     1021 gggtgcaaac tttccaagca gaaacgattg gtgaactaaa aaaggtcgct gattacttct
     1081 gaatcaattt ctccataaaa taaacgatag ccttctcgaa aactcgagga ggctatctga
     1141 cgattcaata ttcattaatt taaagtttgg gtgactatta ctaactatta cttatttctc
     1201 atttaaacga tgtattccct aacctctaat acatccttgt aactctgaag ttacgccagt
     1261 tttgaataaa tttcataaaa acaaatgaaa agtgtcgcat aaaaatatct ttcgtgtttc
     1321 tctagtataa cagaataaga agaacaagtc cagaagggat atttttagtc ttttttaaaa
     1381 taacttttca gtcgagtttg agtttatgat agttgttttt gaagcgatac ggcaagtgaa
     1441 aaaataggga agcatgaaag aatctactta atggcgcatt gattcatgtg atagaaataa
     1501 aaaacttttt taaattatat cctcatgatt gtaacaaatc cagtgatttg ttttggtaca
     1561 aataaaaaaa ggccccgatg aagttgtggc aaaagtggtt tgcctcaaaa ataagaagga
     1621 atttccaaaa atcacttctc aaattttgcc acatccccaa ttgggtcaaa tttgtttatt
     1681 tgaatatatt ttttacataa ggatctttaa tagttacatt gtcttttttc atcactttac
     1741 ggattgtttt atccatgaat tccgtatcaa gagtttttgc ttcggtagca attttttcta
     1801 gctcagattt gtatttgtct ttattagaac ctttatcctg tttttttacc atttttacta
     1861 gataatagct ttgctgataa gtcgttggat ctgttacagg aatgatatca gaaacttccc
     1921 cgttttttag tttaaatgct gcttttttta cttcagtagg aatctctgga ttggttgaat
     1981 caaaattcat ttttccatca gtttccttta atgttttatt ttttccataa gcttgaacaa
     2041 gcttggagaa attttctcct tcatttgctt ctttttttgc tttatctgct tcttctttag
     2101 tcgaaaatag aatgatctgt gtactaactt ctggatagaa ttctttccaa gcagtgtcta
     2161 gatctttatc tgttaatttt acattggctt tcaaaccgta ttggaaagcc agcatctgtt
     2221 taaagagttt cttatatgtc tcttctgttt ggccaactgc ttttaattgt tccttgtagt
     2281 ttggaccgag ttttttgatt tgttcattat attcttttgt tacttccttt gaagaaatct
     2341 cttttccgta tacatcatca aatgctttga agacaatcat tttttttaat agtgtttgat
     2401 tatttgattt aacttttggg ttatcataaa aatcctgaac tgtaagtgtt ccacctttaa
     2461 acgttgcaat cgtttcattg gacttgctac aacctgagaa tagtaaaatt gcacatgctg
     2521 aaaggcacat aattagtagt tgttttttca aaaaatttcc catcctttcc ttattaaaaa
     2581 cagtctaaca gaagaattag ggagacagtc tttcattttc gtaaggttgc tgtagatatt
     2641 cttagttcgt aaaaatctat tctctctatg atggagaaca tttacatagt tttcacgaga
     2701 caagaaagaa aagggttgtt aaacttaata taaaggaggg atgaaagatg atcaggatta
     2761 ttcggttgat tggatctgtt ttaatagtct tagttggttt tgttttagga attcttgtca
     2821 ataatgcact tgcggatatg tttattggaa atagtgaatg gattggagca attgccggta
     2881 cggtcggaca tttagctgtt tttttactag ctttatttct tgctagtaag gtcgaaggaa
     2941 aaagaataga agattatggt gtttctgttc gaggaaaaga ctgggggtat ttagcgggag
     3001 gtctcgttgt tggaattgga gtgtttctgc ttattactag tccattatat ttgacggggg
     3061 cgtatcggtt agattcagga aacgcaaaca ttgtgccgct gataacgagt tttattttat
     3121 ttattgcagt gggggcatca gaagagcttt tattccgtgg cttttttcag catcagatgc
     3181 ttagatttgg tccgttaata gcaatgattg gatctgctgc attatttgct cttttgcacg
     3241 gattgaatcc taacatgaca tttctggcag ttttcaatat ctttttagca ggttgctttt
     3301 tcagcgcact tatctacagt acaggaagtt tatttacggc tattggtgct catattactt
     3361 ggaattggac acaaggagct attttgggta taccagtcag cggaacgcaa gaacaaggtt
     3421 ttttctccac tctgatccaa tcgcaaaata caataattac tgggggaaac tttggggctg
     3481 aagcatctat atcaactaca ttattcttgt tagtcttgat tgttggtttg ttactctatg
     3541 catataaaac aaagcggatg aagaaataca ctgctgttta tcaataaaag atgaagaaag
     3601 caaggctgat atcggtcttg cttttttaag tctgtcattg attaaacgaa aaagtaaaca
     3661 aattcaagcg aatacacgac atcttttgtt agattttcac aactgactgt cgtgttctca
     3721 tcattttcat tttatataat tcctttatca aatagaaggt aattatatgg agagtgatag
     3781 aaatgaagaa aacagcgacc gtattatttg ttattcttgg gctgagtata attgcagcaa
     3841 tgattggaat cagtacttct gcaaatgaac gggcaaatga gaatctttca tttactgtga
     3901 aaactgatcg gattgtttat gacatgacac aacaagtcat tactattcca gtcaaaccaa
     3961 acaaaagcgt gaatgctagt gatgtacatg ctgttctaac atatggttgg gatggtaatg
     4021 gaagttcaga aaaagttatt ggggaagttt atttaaaaga cgtacaatgg accgctggta
     4081 ttgagtatac aataatgatt tctgcagaac tatcgattga tgagatcaag agtaaagata
     4141 aagttgattt gattgttttt tatgatggac agatgacgat cacggagaat ttgaaaccaa
     4201 gcagttggac agttgttgga ccgccaagca gtacggatag cacaagtagt gaaagcagta
     4261 cagaaaacac gagcggtgaa agcagtacag agagtacgag cagtgaaagt agcacagaaa
     4321 acacgagcag cgaaagcagt acggagagta cgagtagtga aagtagcaca ggaagcacga
     4381 gcagcgaaag cagtacggag agtacgagta gtgaaagtag cacaggaagc atgagtagtg
     4441 agagtagcac aggaagcacg agcagcgaaa gcagcacagg aaaagtaaat aatgaacttg
     4501 atttggtacc tatcatacca actaaggaac aaaagagaaa tgcagggagc aacctaaaaa
     4561 gtgtgctaaa acagcctgat tttgtttctc gtggaattga tcatcaagaa aaagcgaaag
     4621 aaacagataa tagaaaaagc gatttaccag aaactggtag tagaaccttg aacaagctaa
     4681 ttacatggat gggtgtgtta ctcattttga ttgtaggagc aagctatttt cgttctctct
     4741 tccatcgagt gaaataaaga ataaaagcaa cagatctaaa attgaataaa acgatagatt
     4801 tttgagtgaa tatttcattc gagagtgctg tattgcctgg cgaagttgta gctttgccag
     4861 atctattttt gtttactaac atatttgaga aacaggaaat aacaggaaca caaatggaaa
     4921 ataagagcaa gaataattga caatgcacac tgcgcatggt agtatctctg aaaaatcagt
     4981 aacgaatcta tttgtacgtt attcatgtag ctgtcggaat aagtctaaat aatagataaa
     5041 aaggagtatg tactttgttt gaagaattta ttgatatcaa tgaaaggcaa gtatatcaat
     5101 ttttgaacta ttgttatgaa agagacgaga aattatatgt ggtgaaggat atcgctttag
     5161 atctaaacta caccctggct aagatgaatt cggtgattca acaagcagaa tcgttttgcg
     5221 aaaggtatcc agaatataaa ttatcttttc tttcagaaaa caagatgata aaagtcgagt
     5281 tttcttctca gtttttactg agtaaagttt attctatact tttagaggga acgattggtt
     5341 atattttact ggattcactt tacaaaggta cttatcaatc cttagaaaat cttagtcaaa
     5401 aaattatata agtttgcgta cgattcaaag aaaattaaga gaacttaata ctattttgaa
     5461 aaactatcat ttatcttgca gcttgaaaaa aggtaatccg atagaaggag aagaataccg
     5521 tatccgatac ttttttcact tgatgtattg gcagtcattc gacgaaaatc attctgatat
     5581 attatcaagt ataaaaaata aacgtcaact attagaaaaa tttgcgaaac atgcgccata
     5641 tacgcgtagt atagatagaa agaaatggat cagcctacta agaatttcgt tgcaacgaat
     5701 gaaacaaggc cactcgatta cgtatgtccc agaagagatg aacaaatttg tgcatccatt
     5761 gataacaaaa gaagagttta cgcattacat actagagcct ttttttgaag caaacttctg
     5821 gtcgatgaat cacatcactc caacagagca agcttacctt tattttatgt ttggtgtaat
     5881 gaacacctat ttggaagaag atttgttgga tactccagat aaagtacaag aaagttgggg
     5941 ctggattgaa tcgtctattg tagaaaattt tgaaaaaaac ttcaagattt gttttacaac
     6001 ttcagaaaaa aattatctat atgtcaattt ggcaatgatc catttgtatt caagggtttt
     6061 tgggacaaaa aagaaggtgg atgcttttgg aaaatcagca gaagacagag attttcagca
     6121 tatatttcca gtgatgtatc caatcatgca acagtttttt agtggggtag ctcgcagaat
     6181 tccagagctt aatacttatt atgaaaagaa cagacggttg atttttcaat actgtatgtt
     6241 agtcagggat atttttataa aacacgaaca acctgtcata ttctatattc aatcaaagta
     6301 tggtaaaaca caggaattat gggcaaagaa aaggatatta tctatgactg aacgcccaat
     6361 aaaatatagt gcatccgctg atcagttacc tgatattgtc attagcgatt atcctatcga
     6421 caaaaaggca tatgatcaaa cgaatacaca gatattttac tggaatggac aacctacaaa
     6481 aaatgattgg gcgcgtctga aagctgaaat cataaaaatc aggaatgaga aaaatagatc
     6541 catattggaa aaattggatt tcgttaatta agaaaagcag attgtttatg ccaaaattaa
     6601 gacattgcaa aaagattatt ttctttaaaa tcgacaagat gaccagaaag caagagttcg
     6661 ttgagctctt gctttctttt agttttagaa aggaacatcc tgattttatc ttcgctaaat
     6721 ccagtcaaaa gttttgcaga ttggtcgtag ataatcggtt ttttcaataa agaaggggat
     6781 gtacgaatct caatatatag ttcactcaaa ctgagttcat ctattctgtg aacaagattt
     6841 ttataactaa tagaatgagt agagagcatt tcggtcacgc cgttgtttgt caattttagt
     6901 aagtggataa attcattttt ggttagtggt tgacgaatta agttctgttc gataaacggt
     6961 atatcatgga cgataagcca acgttttgcg cgtcttgaag acgagcagcc agaacctgta
     7021 tacaatttaa tcatcataca ttcctccaat agctaagtat ttatgaaata ataacatgaa
     7081 gaaatagtcg aaaagtgcca tttagaggtg taaaataaga aaatgttgtc acgtataatg
     7141 gaactagcta gataagaaat actattaaat caagaaaaaa gaaagaaggc acaagtatgt
     7201 caaacacagc cgacgcaata gtagtgatcg atttacaaaa aggggtatgt tttgattctt
     7261 cgccgataca cgaattagaa acagtgacag cattagtgaa taaaagaatc aaaacatacg
     7321 aaacgattgg gaaaccagtt attttcgttc aacacgaaga tgctcaatta gtgccacaaa
     7381 caaaaccatg ggagctattg tcggaattat ctattcctga gcacagttat tttgtgagaa
     7441 aaacccatgc gaattcattt tataagacta atttgagtgt tttattaact gaattggact
     7501 gtcattcgat cgaattatgt ggggcacaag cagagtattg tatagatacg actgtgaagt
     7561 ttgctcatgg attaggatac cagcttatga tgtgtagagg agcaacgaca acttatgata
     7621 atccttttat gaaagcaaag gaaacaatcg ctttttacga gaaaatttgg gataaacgat
     7681 ttctgacatt tttggagcaa taagttgttt cttcgtttaa aaaaagcaga atggatactg
     7741 gtttatccat tctgctttat tacgtaaaat gagaaaataa tcctgttcta tttttatggg
     7801 ataaaccatt tattttttgt ataaaaaatg tttgcgttta catataattc tcctagaaac
     7861 ataaaaagga gtgaatagct tgagaacaaa acaagtatta aagggagcgt tggtcatctt
     7921 atttgctgta agtacaagcg tcccgcaatg gacagtatat ggagagagca tttcttccga
     7981 atcggaatct cgggaggtag aaaccacaga aactactgat gctggaaatg aaacgaaatc
     8041 gagtacttca caaacagaaa caaatactga aacaacggtt gagacatctg ttactagcac
     8101 agatcaagaa acgaccgcta ccacagataa ccaacaaaca gaagaagcaa cagctacaaa
     8161 aatgtcaaac gacgaatcct cttctgctaa agtaagtgac aaagagaatg ggacagaaaa
     8221 tgatgatatc ggtaaaggtg aaaatttcaa tggtaaagaa tatttgaatt acttcggtaa
     8281 acagatgaca gaaatactta atggaccaac agaagacatc aagtcaaaat taatagagtt
     8341 attagtcact gagcttgctg ttaatgatta tggtgtagat aaagagatta tcaaacatct
     8401 gattgctact tatctattgt acgatatcgc ttttgcaaaa aacagtgtgt tgatgtcgat
     8461 gcttggaggt acagatgatg ggacaacgcc tacagaaaac acaggcttct atccaaatac
     8521 aaaacaactt catcaaactg tactaaacaa acaagatggc aaaatgatgg atgtatatgc
     8581 atattatgtc gatcaaggtt cagataaaac agtcatgatc catggcggat ttcgagggaa
     8641 ttggaataac gggatcgtta cagaagaata caatgatttt tataaggccg ggtataattt
     8701 attatttgtt gattccagag cgactggtaa tagcggaggc gattatgtga cctatggaca
     8761 atatgagtca gatgacgtcc tttactggat aaatcaagaa gttcgagagc ggccatctca
     8821 aaagatttta ctttatggtg gttcgatggg agcagctacg atgatgtctg tattagcgaa
     8881 agatattcct gtcaatgtca aaggaattat tgaaaattgc ggatttgcat caattgatga
     8941 acaactacgt tttacgtaca gccagacagt agcccccgca ctaccggatg ccataaaaaa
     9001 tcagttagac atcattgggg atcaagagca tgaagattta tttatgggcc ttttgaaaca
     9061 atactatttt gatcaggaga tgcatttgga tacaaaagct gctctaccta cgattggaat
     9121 gtcagattca ttgcctaaat tgattatcca tggaacagca gatgatgtag tacctgtgtc
     9181 taacgcgcaa aaattatatg agttatcagg agggtataaa gatcttttat tagtagaagg
     9241 agcagggcat ggaaaggcac aggaagttga ccatgctgcc tacacgaaac atgtaacgga
     9301 ctttttgaaa gtagtgttcc atgatcagat aaacgtaaag tatgtggatg aaaacaataa
     9361 aagtctactc aaagaccagg acgaaatcca gctttacgga gcttatggag aaaattatat
     9421 gacagagcaa aagacattcg aaggatacga attagcaaat gttgaaggac cgactgaagg
     9481 gatttttaat gagacgatac caactattat tttctattac aaaaaaatcc cagtagctcc
     9541 accaaaaaag caagatccga cagagaatgc agacaataag ggaaagaatg aacctcgaga
     9601 ggatcaatca agtgatgata cgcaaccgat tcagatgaca gtgaaagtac aaaaaaagac
     9661 agatgcaaaa aaaagaatgt tgccgaaaac tggagaaaag aaacattcgc ttctcattta
     9721 ttcaggtgga tttttatctg ttagcacgat tatcacatgg atttggcgaa gaaagagagg
     9781 gtaaatgaat gaaatggttc aagcgaatag tgattgcagt accagttatc atcgtagtta
     9841 ttttagtcac tgtatttttg attaatcaaa tcacaccaaa gccgattagc ttgcttgtta
     9901 gaaaacaatt tgacacaagt gaaaaagaac aagtatatgc ccgaccagat gatttccagc
     9961 aaaaaatcga acaaatcgat ataaaaaaag aaatatccta tccttcaaag tacaaaaata
    10021 ataatatgga tatctacaca ccgaaagtgg aaaagaaaaa attaccgatt cttttctgga
    10081 tgcatggagg cgcatatgta ggaggggata aaaatgattg tcgtgattat ttggaatatc
    10141 tatgttcaga cacgcagcaa atcatcgtaa atatagatta tgaacgatca ccggaagcaa
    10201 aacatcctac accagttatc caattgaacg aagccattca agcagctaaa aaagagataa
    10261 aagatcaagc agattggtcg aatatttcga ttggtggaaa ttcggcaggt gcacaaatta
    10321 gtggagaata tttgttagca cttcaaaatg aagaaattaa aaaagctgat catcttgatc
    10381 cgactttgga gaacaagcag attacgaagt ttatttcttt gtctggtctg ttggatcctt
    10441 ctgcacttac tcaagtaagt gataaaatct ctagctttct ttatgaaaaa tgtggatggg
    10501 cttactttga taataaggac tttgaacaat cagagaaggt caaaagttta gccttggtca
    10561 gacatgcgga tcgttggacg caaaacacat tcttaacgga tggaaataca aatacgttta
    10621 cgaaacaaat ggaagaagta acttctgtat ttgagaaagc gggggtgaaa gtcacaaaag
    10681 tagattatgc ggagaaaaat gctgttttga accatgaata tcagtttgat ttcagcaata
    10741 aacaagcgca agaaacatat caaaagttag taatgttttt caaggagtaa agtcttatga
    10801 tgcaaaagag gttgtaatct gtcacttagc tccaagtcac aaggagcaat ttcaataaac
    10861 aagcttttca tggtttatga atattgtaac ttgtgactga ggagttagag atggcaaggg
    10921 gcacctttta ttcgaaattt gggaatatca cacgattatt gtcacgatct cttttacgta
    10981 aattttataa gtcagatttg ttgttttgat cgtttaaaaa aagcaacaaa tccgaagcct
    11041 gcggaggcaa gaagggacaa aaagccgagt ggtaagcaaa agaatgctgg ttccactaat
    11101 attccgttgt tattgatata aggctgggaa caaagatgaa tagcaaataa aatcaatgca
    11161 ctagcgaaaa aaagcaaaga aatatggata gctttcataa gcagcacctc cttttttata
    11221 attgtatgat aaatagatta agaatctata aatgtgacaa gtttgttagg atgtgaataa
    11281 aatgatcatc tagcgaactt gaaacaaaga tggctaatga taagcaatta atgattcagc
    11341 aatcagatag gtacaaagtt taccatcata cgtctagacc aataatagaa aatatgggaa
    11401 tataatagaa aagatttagg atacccccaa aagaatttcc ccaaaaattc gatgtatcct
    11461 aaactttttt attttacata acaatcatat caactgtgaa ataaaattac aaatgataac
    11521 ctaaaatgag gccaaaagcc agtgttatca aggaaatact taattcacca aaattttttt
    11581 taatttttta gaaaataaga aacaaaaata ataaaaaaaa gaaaagataa attttttact
    11641 actgtatact aactttgtta tcgcatctaa caagtaactc gaataatacc aatacaacct
    11701 tctccaaaga tgcgatgaca tacgttctaa cccaccgact attgatgttc tgtaccaccc
    11761 ttgcagaacc aaaaaagcca ctaccaaata tttggtagtg gctttttttg ttttatggat
    11821 ttttcgcttt ttctgttatg gtgctattct ttgatttcag aaatagtatg tcgaacttta
    11881 gcgatagaac gacgtgattc tttttcgttg taaaacatca aaatcacata tagagagtcc
    11941 aaaatgctta gttgagaaat acgggaagct aaagcttcag atcggaattc tgtttcttca
    12001 gaaatagaaa taaaaacgac atcgccaagt ttagcaaggg aagaattagc ttggctagta
    12061 ataacgattg tttttgctcc agtttttctt acggtttcag caatatggat tgtttctttt
    12121 gattgccctg tgtgagaaat caaaacagca cagtcttttt ccgtcaataa agaagcttcc
    12181 atcaattgaa tatggtaatc cgtgctgtga gaagttgcga taggggaacg caaaaattta
    12241 tgataagcgt cagttgctac aatctctgaa ccgccaacac cgaaaaaata gacacgttta
    12301 gattcattga taatagtagc cgcttttttt agatcctttt gttgtaacaa cttttttgtg
    12361 tcatttaaag ttgaaatact agactcgaac accttttgcg ccatagtgag ctcagtatct
    12421 gttttctgaa tattttcgtg gatagaaata gcagatagat cattttcttg gatcaacatg
    12481 gccattttaa aatctttaaa tccattataa ccaagttttt ttgtaaattg aaaaaaagta
    12541 gagtcagcaa tgcccagttc acttgctaaa ttgctaatag gactatgact tactttgctt
    12601 ggattttcta aaatataatc ggcgatagcc tgttcttttg ggctgaagtc gttgtacatt
    12661 gttttgattt gtagtcttat ggagacgctc atgagaattc ctcattttca taataagatt
    12721 atcatttttt ccgttctcat taatgttaaa gatacaagga gagtaggaaa aaatcaaaca
    12781 aaaaatattt ttgtaaaatc tatttacaaa aaaatttttt tgtataatga gggtataaga
    12841 aatggaggcg ctatcatgga aattggtgta attggtttgg gaaaaatggg attgaattta
    12901 gctttaaact taaaagagca ccatcacaaa gtacaaggac tcgacatttc aagtacagct
    12961 gttagtcagg caagagaaaa tgacattcct gcttatcaag agatctctga atttctagct
    13021 acttttgagc agaaaaaaat catctggtta atgcttcctg ctggagagat aaccgaaaac
    13081 atgttaagaa aactttttcc aaaactcgga ccaggagaca ttattattga aggcggaaac
    13141 tcttattata aagattcaat ccgtcgagca gaagagtttc agaaaaatga aatcggttac
    13201 ttggattgtg ggacttctgg aggaatcgaa ggagcacgac atcaagcctg cttaatgatt
    13261 ggaggagata aagaaacttt tctatccgtg gaacaattat tcaaagatgt agcaattgaa
    13321 agaggatatc tgtatgctgg tgcatccgga agcggacact tcttgaaaat gatccataat
    13381 ggaatcgaat atggaatgat gcaagcaatc ggtgaaggat tccaacttgt acaaaaaagt
    13441 gagtatgctt ttgacttgga acaagtcgca cgtgtttgga atcatggttc ggtgattcgc
    13501 ggctggctga tggaaatcat ggaagatcag ttaaacgaac atcccaattt aagtaattat
    13561 cggggaatcg tttctgcatc aggtgaagca aaatggacgg ttgaaacagc gctagaaatg
    13621 gctgttccag ttccagtaat tgcaactagt ttgttcatga ggaatctttc acaggaagaa
    13681 gatagttttt cagcaaaagt agtttcagca ctaagaaatg ggtttggcgg acatgaaata
    13741 gttgaaaagg gtgagtaaaa tgccagatta tcatatagca atcgttaatt ccagcagttt
    13801 cggaagagtc tttccacacc acttggaaag attaaagtca gttggagacg ttcatttctt
    13861 tactgttgat caagagattc caggaaaaga actagcggag ttacttaaag gattcaatat
    13921 cattattgca agtgttacgc cattttttcg acgagatttt tttgaaaaca aagatgaact
    13981 gctattgatt tcccgacatg gtattggtta caacaatatt gatttaaaag cagcaaaaga
    14041 acaccagaca atcgtatcta tcatacctgc tttagttgaa cgaaatgctg tagcagaaaa
    14101 taatataact aatttgttgt ccatgcttcg acgcacgaca caagcacaag caagagtcaa
    14161 ggacgatcga tgggaagatc gggcggagtt cgtcggtagg acgctgttta acaaaacagt
    14221 tggtgtgatt ggtgcaggaa ataccggaag cggagtaatc gaaattttgc gtaacggttt
    14281 tcgctgtgat gttctagctt atgatcctta caaatcagat ttatacatcg aaagctttgg
    14341 tgcaaaaaaa gtagaacttc aagaattact ggaacaatca gatatcattt gtttatgtgc
    14401 gaatttgaca gaagagaact atcacatgat ttctgataaa gaagtcgatc agatgaaaga
    14461 aaatgtttat ctatctaata gtgcaagagg cgctctagtt gatgaagaag cgatgattaa
    14521 tggtctgaaa agcggaaaga tcgcaggata tgccacagat gttttagaag aagaaccggg
    14581 acgtcaagat catccgtatt tagcgtttga acaagtagtg attacgccac atatatcggc
    14641 ctacacaagg gaatgtttag aagcaatggg tgaaaaatgt gtcagagatg tcgaacagat
    14701 cagtcagcac cttcttcctg aacgttctgt ccagacaaag agcaactata taaaaggatg
    14761 atgaagaaaa tggtgaacgg tttaaaagtg aaaacaggcc ctcaatttta tttatatgaa
    14821 gaaggtggta tttcaaaagt ttctgatctc ttgaaatcat acggcgcgaa aagagtactg
    14881 gtcacccacg gaacggtatc atgggaaaag gcgctaccta aattagtatt tttgaatgac
    14941 gaaacgattc aattttttta tcatcgttat tctggagaat gtagttatgc tgaagccaga
    15001 cgaatagcta caatcatcaa aaaaatgaaa tcgatttcgt agttggcgtt ggcggaggaa
    15061 aactgaccga tctagtgggt tatagtgcca atcttgcaag aatcgatttc ggagtcatcc
    15121 ctactttagc tagtaattgt gctccatgga cacctcttgc tgtcatgtat aaggagaatg
    15181 gacaagcaga agggaaaaca gaacactttt acagacaagc tgcttttttt ataacagatc
    15241 cccaattagt gattgattcc cctgttccat atttcattgc cgggttagca gatacactag
    15301 caaaatggta tgaatctgag ctcatcttag aacaagattt tttgcaagat gaatcatttt
    15361 tgaagctagc acaagatact gcgaagatct gcaaagaaga gattttgaat cattctgcta
    15421 aagcaatcca agatatggca caaagaaaac tgacatcgga gttcaaacag ctttcagaga
    15481 ttatctttgc tgttgctggt ttagttggag gattaggaga taaatacgca agaaatgtgg
    15541 cagcacatgc tatgcatgac gctatcagta aatatattcc ggaaagccat cgttttctac
    15601 atggagaaaa agtagcgtat gggattttct atcaactggc actagaaggt cgctgggaaa
    15661 aaatagacga gttatatcct ttgtatcggg aattagaact gccggtttct ctagaacaaa
    15721 tgggattatt tccgaaagat aaacagataa tagataaaat ggctgaattt attgattcga
    15781 aagagaaagt gcatttgatt cctatagagg taacacagaa atcactgata cagtcaatga
    15841 tggaactgga aggttatatc agaaagatct gaaggaggag gacacatgga aacattaagt
    15901 gtagtacaaa gtttgttgat tgctttatgg gtagccgcgg ttatgtcccg ctggttaggt
    15961 ggaggagcca cgttgacctt acgtttttct cctttgatga ctggattagt cgtcgggttg
    16021 atcatggggg aagttgcaca agcgatgatt gttacagccg ctttgcaaat gatttatatg
    16081 ggggtttttt ctccaggagg atcaatgcca gctgaaccat ctattgcagc agctatcgca
    16141 gtaccagtcg cattattagg ggatctgtcg ccagaagcag cgattgccgt tgcggtgcca
    16201 gtcggattgc tcggaagtta cttgtatcag tttcgttttt tcatcaatac ttttttaggt
    16261 aaatatacag acaaagctgt agaagatttg aatacaaaag ctatggcccg atcaattatt
    16321 ctttatccaa cggttgcctc atttttatta ttcgttccgc ttgttttcat tgctttatat
    16381 ttaggggcgc cattgatcgc tgatgtaatc aaagctttag agggaacagt cgttatccac
    16441 attcttgaag tagtaggtgg agggttagcg gcaatcggta tcgcgacaac tgtatacgtt
    16501 atcggtcgaa aagattatct ggtctttttc ttccttgctt attttatgag tgttgttttg
    16561 aaatctttag atattacgat ggttacttat gccatttttg ggattattat cgctttgatc
    16621 tttgtccagt cgcaaaaagg gaaacaagta cagcctgcca caagcaataa caattcagca
    16681 tcttttgatg acgaagatga ctatgatgac ggattttaat ttaggaggaa tgggaaatgg
    16741 ctgaaataat ggataatcaa acaggtgctc ctgaagaaat cacgaaaaaa gatgtaacaa
    16801 aagcctatct gagatggcat tttgcaaacg aaattcctca ctcttttgaa cgttacttgg
    16861 cgccatcact actatacgga atgatgccaa tcttgaaaaa actttataaa gatgaagact
    16921 ctttaaaagc agcatataaa cggcagttac tgttcttcaa cactcaatta agctggggtg
    16981 gcggtgtgat tactggccta atgtcttcta tggaacagca aagagcagaa gaagagtatg
    17041 aagaaaaaga gatcttgatg caggatgact tgatgtataa cacgaaagcg ggattgatgg
    17101 gggcactagc aggaatcgga gatgcaatcg attcaggaac cgtccaatat atttttatcg
    17161 caatcgcggt accttgggca caacagggaa gtcctttagg cgcacttttt ccttttatct
    17221 gctttgggtt gtatcaggtt ttactagggg tatttttcgc aagacaagct ttcactatgg
    17281 gccgtaatgc tacaggccta aagcaaaaca ctggtgttca gacagcgatt gaaatgctat
    17341 cagttttagg gttgtttatg atgggggtgc ttgcgggaaa ctatgtaaaa gtagaatctt
    17401 cattgaaatt tgcgatatcc ggacgtgagt ttgttatcca ggaaattctt gatcaaatcg
    17461 tgccagggat attaccttta gctgtcgtaa tgggtgtgta cttgttctat acgaaaaaag
    17521 gattgaaagt cacacaagca ttattatggc tgactggaat attaattgtg ctagcaggaa
    17581 tcggtatttt ataaaaagaa gggaacgtga ataagatggg tgtagtcaat ttagcgcgtg
    17641 tggatgagcg tttgatccat gggcaagtaa tggtaacgtt atctcaaaaa agtggagtga
    17701 attccatttt tgttgtagac gaagttgtag caaaagataa atttatgaga gatctttaca
    17761 aaaatgcagg aagccgtaca ggacaaaaaa caattgtcat tacaccagag aaagctaaat
    17821 tctattggga tgaatatcaa tttaaagaat acaattgtat tttgatcgca aaaacagtcc
    17881 aaacgattta cgacttagta aaacacggaa tccccatgga agaattgaat attggaggga
    17941 ttgctcaaaa aaatcctgat acagatcttt tagtgacgaa atctgtttat ttaaataaat
    18001 cagatgctga aaaattgaaa gaaatgaatg aacaatatgg cgtaaagaat atttattttc
    18061 aagccactcc atctgcacct aaaacaggat taaaagatgt attgaacaag tttgatttgt
    18121 cataaacaaa tgcttagaaa aaagataaag agggcgaaaa aatgaagaag aacgagctgt
    18181 ttcgtgattg ggaatttcgt tatcgctata tttaccgtaa gcgccgtaca aaaaagagta
    18241 aacagcgttt tctttcagca ttagtatcag atatttacag tatgagaaca gatgtgacag
    18301 tcattgctta cgatacgcta gcctaccgat caaaaaatat ttatgttgga gatatcgaaa
    18361 aagcagagaa agttatctgt acttattacg atacacccgt tcatgcatta ggaagttatt
    18421 tcatgtttga ttggaaagac caaaggaaaa aaacaatcta ctctatatta ttgtccttca
    18481 ttttgttgtt cagtttaggt tggtggggaa tgatgatata taacaaaaat cctcatcacg
    18541 tattcgatct gctatccgta taaacaggta tcactgtact agcatttggg agctactttt
    18601 tcttattggg aaaagcagct cgcggatggc ctagcagaca aacttttatt cgaaatactt
    18661 catccatatt aacgatgcta gaaatgatcc gtacgattga tgatccgagt gtagcgtatg
    18721 cgtttgttga tgaaggctgt tatggagaaa agggcttaga cagcgtgaga tcgggtatga
    18781 aaaaagaggc tattcttttt tatctagact ctgttggggc agatacaccg ctacagtttt
    18841 cgggaaatta tttttcaaat aaagaacagt ggctgaaaca agttgataag ttgaaagaaa
    18901 aaaacgtcaa ttatattttt agtgcaagaa aaaagcaagc gcagttcttc tatttgacca
    18961 aaacagattt acgagggaaa acatttaatt ggcaaaatgc caatcagatt attgctcttt
    19021 ttcgataagt aaaaagtgca aaacaaggag tgagaagact aatgttggaa atcatgatcg
    19081 ttacacatgg taagttgagc gaaggactga aagatgcggc ggaagtaatt gtcgggacat
    19141 cgagccatat tgaaacaatg tgcttagagt ctggggagga catccaaaca ttcggagaga
    19201 aggtcaaaga aaaattaact atgatcaaca cagcagaacc catgctagta tttgttgatc
    19261 taattagcgc aagtccgtac aatcaagcgc tcttggcagt caatgaactg actgaagaaa
    19321 agaaagcagc cacttatgtc atagggggcg tgaatctgcc aatgttgtta gaagcaatca
    19381 atcatcagat gcttcacaca ccgatagaag aagcggtgga acaaattgtc aaacaaggaa
    19441 aagataattt aactgtttgg catgcttcag cggaagaaaa aaaattagat gaggacgatt
    19501 tttagaagga aggctgaaaa gatgaagtgg ggatttagat ggtatggtga aaaaggggat
    19561 tcgatcccat taaaatatgt tcgccaaata ccgggaataa atgggataat tggaactttg
    19621 ctgactaaaa tgcctggaga tgtttgggag atttcagaaa ttgaggagtt gaaagcatct
    19681 gttgagagcc aaggactgga actgcttgga attgaaagtg tagcgatcca cgatgcaatc
    19741 aaggcaggga cagatgaacg agattactat attgataact atatacgaac aatcaaaaac
    19801 ttgtctcgat gtggggttcg attgatttgt tatagcttca aaccaatttt tggatgggcg
    19861 aaaactgatt tgtttaatga aaacaacgat ggaagctatt ctcttttata cgatcagaaa
    19921 atcgtcgatc agatggagcc aggagaaatg tatcaattga tacacagcca gtcaaaggga
    19981 ttcaaattgc cgggttggga agaagaacgg ttgaaaaaat tcgctcagct ggaaaaactg
    20041 tatgaaggaa tgaccgaaga agaattattt gaaaatctga cttatttttt agaaaaagtg
    20101 attccagtgt gcgaaaaagc agatgtaaaa atggctatcc atccagatga tccgccatgg
    20161 gaactttttg gttttcctag agtgacgaaa aatcttggcg acttgaagaa aattctaaat
    20221 gcagtagatt ctccatataa tggactaacg ctgtgcacag gatctttagg ggcaaatccg
    20281 gaaaacgatt tagttgaaat catccatgag gtaggttcgc gcatccattt tgtccacttc
    20341 agaaacgtgg agtttttagg agaaagaaag ttcaaagaaa cggcacatcc ctctacagaa
    20401 ggctcccttg atatgtatca tattatgaaa gcgttggtgc aagaagggtt cgacggtcct
    20461 atacgccctg atcatggtag aaccgtgtgg ggtgaagtgg ctatgcctgg atatggcctg
    20521 tatgatcgtg caatgggact cacttacatg caaggtctat atgaggcgat aagtaaggaa
    20581 aaaccactgg gagagcatta tgaataaaat caagctcttc atctttgata tggatggcct
    20641 tttattagaa actggacgtt tagcttatcg tgcttatgta aaatcagcac aaaagtatga
    20701 ttatgaaatg cgtaaggaag tctattattt actaactggc caaacagaaa tggcgataag
    20761 agaacaaatg ggcattttgt acggagaaga tgttccatat atcaaatggc gagaagcaat
    20821 caatcaatat aaagaaaaaa tcgtgaaaga agataagaga gtatatacga aaaaaggagc
    20881 agaagaaatt ttatcttttg caaaagaaag aggaatccat acaatcgtgg cttcgtctaa
    20941 tacacgggta aaagtagaaa tgtatttaaa aatggaaaat ctatatgcct tgtttgatca
    21001 aatcatttca ggggatgatg taaaaaaagg aaagccggaa ccagagatct ttttgaaagc
    21061 ttgttcaaaa atgaatattc ctccaagtga agcacttgta tttgaagact cgattgcagg
    21121 gatcgaagca gcaaggcgag cgggcatttt gtcttttctc atcaaagatc atttgatagg
    21181 gttgcccgat cataatggga agcataagtt gaaagtagat gtaagtttat atgaatccaa
    21241 taaagcagac tatgaatttt atgacctgca tcaagcaaaa cgatttttag aaagtaaagg
    21301 gttgtatctc taataaagag gactattttc tagtcctctt tatttattgt cctgcacgac
    21361 tgagggaaca gatgaaaaag aagtaatttt ttattttgaa gtatctacct tatttgtagt
    21421 gaacaattta gaacaaacac aaaacgcatc tactgttatc taccagtaga tgcgtttaaa
    21481 gagaagaacg agaatcgtcc atatgagggt ttccactcct tatgcagatt tttttaattc
    21541 ttgcactgct tgttcgatcg taacacctgt cgctacatga ttggtatttt ctttgtcaaa
    21601 aacgacaaat agattcaaat ctgtatccaa ggttactcga tattgtatat ctttgttata
    21661 gaatgtcata gccattctcc tttctttaaa tacttgctga aaggcaacga acgcgttgat
    21721 aacgaaattc catttataag tataccacca attgaaaggt tttcatagtt aagaaatttt
    21781 aaagtcaaaa gttgcagata aaagcaaata aacaagaaaa caaaaagtga atcaatcgcg
    21841 gttattcaac tttttcttcg attcgaagac aatagaagac aatgaaattt atatttcatt
    21901 gcattatcct tgtgttatta gaaaaagcat gatatattaa atgagtaatc tattttgaaa
    21961 cgtaatttga gcgatatatc tacatagata aaactccttt tgcctatcaa atatttattg
    22021 caacgaaaaa acgtacattg atacgtatca ggaggaattt atttatgaat aacggtacag
    22081 taaaatggtt caacgcagac aaaggttttg gctttatcac tggtgaagat ggaaatgatg
    22141 tatttgcaca tttttcagct atccaaggtg acggcttcaa aactttagaa gaaggccaag
    22201 cagtgtctta cgatgttgaa gatggtcaac gtggccctca agcaacaaac attgttaaag
    22261 cttaataaaa attgactttt aaccgtcttt tcgaaagaaa a
//
LOCUS       DAZKLM010000048        22267 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3764
            SAMEA115706913-rid22582563.denovo.048, whole genome shotgun
            sequence.
ACCESSION   DAZKLM010000048 DAZKLM010000000
VERSION     DAZKLM010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706913
            Sequence Read Archive: ERR13196647
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22267)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22267)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287823.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287823.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,004
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,849
            CDSs (with protein)               :: 2,849
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 41 of 96
            Pseudo Genes (incomplete)         :: 52 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 15 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22267
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706913-rid22582563.denovo.048"
                     /isolate="VRE3764"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(154..534)
                     /gene="yajC"
                     /locus_tag="ACG842_001620"
     CDS             complement(154..534)
                     /gene="yajC"
                     /locus_tag="ACG842_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HFQ0248167.1"
                     /translation="MGTLPMLVMFIALLAMWFFMSRSQKKQQQERQNLLDSMKPGDSV
                     VTIGGLHGVVSEIDNDKRTVVLDCEGIFLEYDRAAIKTVKPGTVVTNDATTTVETTEE
                     PAETVEVKTETTETLEETEEKEEK"
     gene            complement(631..1776)
                     /gene="tgt"
                     /locus_tag="ACG842_001621"
     CDS             complement(631..1776)
                     /gene="tgt"
                     /locus_tag="ACG842_001621"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HFQ0248168.1"
                     /translation="MTQPAIRYRLIKKEKHTGARLGELITPHGTFPTPMFMPVGTLAT
                     VKTMSPEELKEMGAGVILSNTYHLWLRPGEDLVEEAGGLHKFMNWDQPILTDSGGFQV
                     FSLSDMRNIEEEGVHFKNHLNGSRMFLSPEKAINIQNKLGSDIMMSFDECPPFDESYD
                     YVKKSVERTSRWAERGLKAHANPDRQGLFGIIQGAGFEDLRRQSAKDLVGMDFPGYSI
                     GGLSVGEPKSEMNRVLEFTTPLIPENKPRYLMGVGAADSLIDGVIRGVDMFDCVLPTR
                     IARNGTCMTSQGRLVVKNAKYAHDFRPIDEKCDCYTCKNYTRAYIRHLIKCDETFGIR
                     LTSYHNLYFLLNLMKQVRQAIMDDNLLEFREAFFEEYGFNKENAKSF"
     gene            1994..2692
                     /locus_tag="ACG842_001622"
     CDS             1994..2692
                     /locus_tag="ACG842_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HFQ0248169.1"
                     /translation="MQSSAELKRQAKESLKGRWGQAVLLNLIPTLIGIATILLIMIPI
                     ILFATTIYDGSASVSEATTSGGGSSGGGGGIISSIISALFMSGISWTYLDILRGQKDQ
                     IEPFKDAFRGFKGLFIGGVISLAVLISIFTTLWGLLLVIPGIIKSYSYSQSYFIYYDI
                     VQETGQTPKVLDTITASRKLMDGYKGQLFWLDLSFIGWHILALATAGIGYLWLSPYIS
                     ATKAAFYEQLPKDI"
     gene            complement(2860..5499)
                     /locus_tag="ACG842_001623"
     CDS             complement(2860..5499)
                     /locus_tag="ACG842_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HFQ0248170.1"
                     /translation="MEEYKQTIEEVKKVTEADPDTGLSSQEVDERRKKQGLNKFDEAP
                     KESMIKKFFRSLSDFTTIILLVAAVISFYTAFATEHGDLFEGLLIIAIVVINSVLAIV
                     QEGNAEKALESLQDMNKQTATVIRDGKVTTVESEQLVVGDILVLESGDAISADARLIE
                     ASQLRVEESALTGESEAVEKDAAFIAEEDESLGDQLNMVFKGCTVAAGRGKAIVTAIG
                     MATEMGKIADLLNENTMQKTPLQVRLNQLGKRISMIALAAAALVFVIGELQGEPLLEM
                     FMTSISLAVAAVPETLTVIVTLTLAYGVQKMARKHAIIRQLPAVETLGTANVICSDKT
                     GTLTQNEMRVRRVWSKEDEVTNIEDSMTNSAMEILKMAALCTDVTVEQEDDDLVIKGN
                     PTEVAIVRAVEENYHTKAELEEKYPRVNELPFDSERKMMTTVHQMGEKYISITKGAFD
                     VLAPRFSSGDVEQAAIVNDRFGKRALRVIAVGYATYDEEPQDISSEALEKDLRLIGLI
                     GMIDPPRPESKGAIKRAKKAGIKTVMITGDHVVTASAIAKELGILNDPSEALSGSELH
                     QLSDEELDARVKALSVYARVTPEDKIRIVKSWQRTGAVVAMTGDGVNDAPALKASNVG
                     CAMGITGTDVAQSAADMILTDDNFATIVDAVSQGRSVYQNIRKAINFLLSCNISEIFI
                     VLIAMLLGWGAPFTAVQLLFVNVVADGLPGFALGREPAEHGIMDQPPIPKNEGIFARG
                     LLQKIAINAGVFTIVTLFGYYLGSYVDTISPWVDASQHVGQTVAFLILAYSSILHVFN
                     VRSSQSIFKVNLATNKALVEMALLALAITTAVALLPFTQELFGLVHISLNHWFLVGIL
                     SIVPIAVNELIKFHRLPEAEEEE"
     gene            complement(6118..7149)
                     /gene="queA"
                     /locus_tag="ACG842_001624"
     CDS             complement(6118..7149)
                     /gene="queA"
                     /locus_tag="ACG842_001624"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HFQ0248171.1"
                     /translation="MLTTEDFDFDLPEELIAQTPLKDRDHSRLLVLDRKTGEMEDKHF
                     HEIIEELQPGDALVMNNTRVLPARLYGEKPDTGAHLEVLLLTNTQGDTWETLIKPAKR
                     AKVGTRISFGDGRLQAVVKEELEHGGRIIDFEYEGIFLEILESLGEMPLPPYIKERLE
                     DPDRYQTVYAKENGSAAAPTAGLHFTKELLDEIEAKGVELVYLTLHVGLGTFRPVSVD
                     KIEEHHMHSEFYRLTEEAAERLNQVRKNGGKIVAVGTTSIRTLETIGTKFNGEIKADS
                     GWTDIFITPGYEFKVVEAFSTNFHLPKSTLVMLVSAFAGRELTLSAYQHAIEERYRFF
                     SFGDAMFVK"
     gene            7342..8516
                     /locus_tag="ACG842_001625"
                     /pseudo
     CDS             7342..8516
                     /locus_tag="ACG842_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628506.1"
                     /note="Members of the family are the ATP-binding subunit
                     of ABC transporters for substrates such as betaine,
                     L-proline or other amino acids, choline, carnitine, etc.
                     The substrate specificity is best determined from the
                     substrate-binding subunit, rather than this subunit, as it
                     interacts with the permease subunit and not with substrate
                     directly.; frameshifted; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015837 - amine transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="betaine/proline/choline family ABC transporter
                     ATP-binding protein"
     gene            8520..9161
                     /locus_tag="ACG842_001626"
     CDS             8520..9161
                     /locus_tag="ACG842_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0248172.1"
                     /translation="MQQFFHQHGSEILSKGFEHLYISGLALIIGIIIAVPLGVLLTRF
                     PKAATIVIGLTSALQTIPSLALLALMIPLFGVGKLPAIIALFIYSLLPILRNTYIGMK
                     NVDWNYRDVAKGMGMTEFQSIFSVELPIAMPTIMAGIRLAAVYVIAWATLASYIGAGG
                     LGDLIFSGLNNYQPDLILAGTIPVTILALLADWLLGLLEHRLTPIALREENEE"
     gene            9158..10075
                     /locus_tag="ACG842_001627"
     CDS             9158..10075
                     /locus_tag="ACG842_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmoprotectant ABC transporter substrate-binding
                     protein"
                     /protein_id="HFQ0248173.1"
                     /translation="MKRILKMTMVLFSLLILASCSFPGLASNTDEDTISITGGITSEA
                     QILASLVAGMVEHYTDKNTAIINNLATTTINHQAMMNGDASISAARYTGTDLTTTLNL
                     PPEKDPKKAFATVKDEFEKRYGQTWFPSYSFENTYVFLVRKDTAQKYHLSKVSDLKNV
                     ADELVAGVDTSWINRKGDGYDGFQETYGFSFNSILPMQIGLVYDAVEAGKMDIVLGYS
                     TDGRIASYDLVMLEDDRQFFPPYDAAPVVDNKILADTPHLKEALNKLGHTISTEKMQE
                     LNYEADNNLVEPSVVAQRFLQENHYFEGK"
     gene            10072..10743
                     /locus_tag="ACG842_001628"
     CDS             10072..10743
                     /locus_tag="ACG842_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0248174.1"
                     /translation="MKQLDKMNVFQQFFYYFQENGSYIFAQFIRHFLISIYGVLFAAV
                     VGIPVGIMISRRRKLANWVIRLANIIQTIPSLAMISILIIGLGLGVNVVIVTVFLYSL
                     LPIIKNTYTGMIQVDKNILDVGKGMGMTARQRLFMVELPLSVSVIMAGIRNALVVAIG
                     ITAIGAFVGAGGLGDIIIRGTNATEGTSIILAGALPTALMAIITDWLLGILERRLDPA
                     SRTSR"
     gene            complement(11284..11772)
                     /locus_tag="ACG842_001629"
     CDS             complement(11284..11772)
                     /locus_tag="ACG842_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosamidase"
                     /protein_id="HFQ0248175.1"
                     /translation="MTRKISKIGASLLVLAVATTIDYRQSEAKTIGNVVYRETAKSML
                     KKAMDNQPESSYWFPEDSLAWSFGKDTDTKYNTSVVPLAERVSKATLPNMNVTQTEEV
                     KVVALSAMNSSINGNASRGIDIFYANVFSYWQYIDQLVYGGALLVKDHRIAKSSCDGC
                     SP"
     gene            complement(11973..12239)
                     /locus_tag="ACG842_001630"
     CDS             complement(11973..12239)
                     /locus_tag="ACG842_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HFQ0248176.1"
                     /translation="MSNYTVAIKNSAKVDLRKIKQTNLKKQFEEVIQTLKEDPYMPTQ
                     SFEKLRPTHEGRYSRRLNRQHRVVYKVDEENKVVEIYSAWTHYE"
     gene            complement(12239..12502)
                     /locus_tag="ACG842_001631"
     CDS             complement(12239..12502)
                     /locus_tag="ACG842_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045927 - positive regulation of growth
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HFQ0248177.1"
                     /translation="MANTTMNPSTARKNFYQLLKEVNENHTEIEIISDRSGNNAVLIG
                     LEDWRAIQETLFLEQTGTLDKVRDREKDDSSFTNIDDIDWEAL"
     gene            13023..13163
                     /locus_tag="ACG842_001632"
                     /pseudo
     CDS             13023..13163
                     /locus_tag="ACG842_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736676.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
     gene            complement(13305..15128)
                     /locus_tag="ACG842_001633"
     CDS             complement(13305..15128)
                     /locus_tag="ACG842_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFQ0248178.1"
                     /translation="MDYLKRLLVGKPLKSAENDEHKLTRFAALALLSSDALSSIAYGT
                     EQIVVVLVALSAAAIWYSLPIAAFVIILLISLTLSYRQIIHAYPHGGGAYVVSSENLG
                     KNAGLISGGSLLIDYMLTVAVSVSAGAEAITSAVPALYGHQVAISVTIVLLLMMLNLR
                     GLRESASFLLFPVYTFILVISLLIVVGLYNIVTGAVPLQATALPGAAVPGVSIALILR
                     AFSSGSSSLTGVEAISNAVPFFKKPRAKNAAATLTMMALILGFFFVGITFINYWYGIV
                     PEKEVTVLSQIGKAVFGHGILYYVLQFATALILAVAANTGFSAFPVLAYNLAKDKFMP
                     HMYQDRGDRLGYSNGIITLALGSIVLLFIFHGSTERLIPLYSIGVFIPFALSQTGMVV
                     KWKKEGKRWLSKSIANITGAFISYAIIAILFVYRLGDIWPFFIIMPIVMFIFYKIHDH
                     YKKVAEQLRLENDAKLHDYDGNTVLVLVGNVTRVNIGALNYARSIGDYVVAMHVSLDE
                     DVEKEKEIEAEFKKHFPDVRFSIVHSSYRSIENPIIRYVDIVSKNAAKQNYTTTVLIP
                     QFVPNRRWQNILHNQTSLRLRLRLSWRENIVVSTYSYHLKK"
     gene            complement(15726..18431)
                     /locus_tag="ACG842_001634"
     CDS             complement(15726..18431)
                     /locus_tag="ACG842_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip family protein"
                     /protein_id="HFQ0248179.1"
                     /translation="MQSIKNTLRLYLLDWKRIFKNPIATLLIIAIMIIPSLYAWFNIK
                     ALWDPYGNTGELPIAVYSADKPAEFQGKEVAIGEQVIESLHKNKQLGWQFVDSKEQLE
                     DGVRSGKYYAGIYLPKDFSEDLLSFTSGDIKKPKIEYTVNEKINAIAPKITDKGASSI
                     QSQITNEFIKTASSTLLKVFNEIGYDIDTNLVSINKVKDMILSTDENLDTIDGYTKQV
                     LELQSQLPEIKEKLNKANEFVDYIPKVDEMGEKVVALNDKMPELKDQAKIILDLQEKI
                     PEIQNAGKQLAEIDGDFASIQETMNQGIDEAKQGLTIIQQVQDILPDVKNLENQASDL
                     ANQTKSGASQLKDAIPSITNSVDVTLKSIGQVATTTGSLIDTVRTAIEDGKLTDDEKK
                     NISNVLNGFIDGISQQQNAIDKLVEFLTKLQTSAGNTKLQPIIDQLNQLKPLLSNLQN
                     RLSALNTAVQNEDTKAIDELLDQIDAVVNNINGIIAGINTGQISSTVTSLLDQVIDTI
                     GTAQGALSKADQIDFDSLLSSTKATVTNAVAILEKYQKEMPAIGQEIHDANTMLNGNM
                     DTIVNGINEGADLYNNELPIIQQKLGLAADFIQNDWPALKNEITGTLTTVNEKMPEVE
                     TAVNAAADLINNDWPNIKAGIQKAADAIRKGEQEVDLGEVIKLLKLDATKESDFFTQP
                     VELQTNALYPIANNGSASTPFYTALCLWVGAVLLSSVATTEYYLGKKDRNNFTKREQF
                     VARMLTFLTMAIAQSLIVTLGNIFLLGVDVQNPVYSVSFAVLIALAFMMIVYVLAALF
                     DNVGKGIAIIILVLSISGGGGNYPIQVSGKFFQMINPFLPFTHAVNLLRESAGGIYWP
                     NATKAIWIMIGLFVVFGIVGTAIYPFIEEKMTKFKEAAHESHIFH"
     gene            complement(18737..19684)
                     /locus_tag="ACG842_001635"
     CDS             complement(18737..19684)
                     /locus_tag="ACG842_001635"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HFQ0248180.1"
                     /translation="MRRFAINGYGEAQDVFEEIDAHPREVGSGHVRVEIKAFSVNPYD
                     VALRLGEMKEIRTLKFPYVPGNDGAGIVTEIGSDVTTVHVGDRVAVHAVGGTYGEEVV
                     LPSAKVAKIPDKMSWEEAAGMVTPGITAYNLINHLTEIQPTDTVMILGASGAVGSSLI
                     QLLHEKGIRILTSASSKNEEKVKKLGASAFAAYDKTNPGLQFADQADLVIDATKGSIK
                     GETGIQIMKPGGRYVALNDLPDLDLRQKKEGFYESFVPRKEYLDAEAFAGIIKAYQKG
                     AFHVFISMNLSASLKHVIQAHQLVEGHPPAGKIILSFEK"
     gene            19874..20143
                     /gene="rpsN"
                     /locus_tag="ACG842_001636"
     CDS             19874..20143
                     /gene="rpsN"
                     /locus_tag="ACG842_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HFQ0248181.1"
                     /translation="MAKKSKIAKAKKQQETIERYAALRQELKAQKDYEGLRKLPLDAR
                     PERLKNRDLIDGRPRGYMRKFGMSRIKFRELAHKGLIPGVKKASW"
     gene            complement(20319..20681)
                     /locus_tag="ACG842_001637"
     CDS             complement(20319..20681)
                     /locus_tag="ACG842_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFQ0248182.1"
                     /translation="MVKRGDIYFADLSPVVGSEQGGTRPVLVIQNNLGNHFSPTIIVA
                     AITAKMAKPKLPTHIGIKATGTGIERDSVILLEQIRTIDKSRLKEKVCHLDRPIMAEV
                     DRALKISVGIEALTPVTK"
     gene            complement(20692..21813)
                     /gene="alr"
                     /locus_tag="ACG842_001638"
     CDS             complement(20692..21813)
                     /gene="alr"
                     /locus_tag="ACG842_001638"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HFQ0248183.1"
                     /translation="MVVAWHRPTKAVIHKKAITENVANEVARLPQGKELFAVVKANGY
                     GHGAIETAEAAVAGGASGFCVSNLDEGVELREAGFTQPILILNMVPYDALTVAVAHDL
                     SVTAGTREWLQAAAAVLEKSKLETPLSIHLKADTGMGRIGFCTPEEVKEAAAFIKESR
                     VLEWEGLFTHFSTADQADDTYWNLQKERFIEVLKKLPELPRYVHVSNSATALWHDETI
                     GNMIRYGVAMYGLNPSGHALPEVYPLQPALELVSELIQVKKLPAGEGIGYGETYITPE
                     AEWIGTIPIGYADGWPRKMQGFSLLVEGNYCETIGRVCMDQLMIRLPQEFPVGTKVTL
                     IGKNADKEITMQDIADQLGTIHYEVACGLGQRIPREYQE"
     gene            complement(21829..22179)
                     /gene="acpS"
                     /locus_tag="ACG842_001639"
     CDS             complement(21829..22179)
                     /gene="acpS"
                     /locus_tag="ACG842_001639"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HFQ0248184.1"
                     /translation="MIKGIGIDAVELPRITRLIEEKPKFIARILTSDEMKLFQSLPFH
                     RQVEFLGGRYACKEAFSKAWGTGIGKVTFQDVEILKNENGQPVVTRSPHEGNVWVSIT
                     HTNETAFAQIILED"
BASE COUNT         6305 a         4763 c         4145 g         7054 t
ORIGIN      
        1 aaatatataa agttatttat tattatttat atgacacacc cgattattca atattgagca
       61 atatatccca tgctcttatc tctttctttt ttgaataaaa aatgaggtag gacatctttc
      121 tgccctacct cgtctttaaa taagctttga tacttatttt tcttccttct cttctgtctc
      181 ttccagcgtt tctgttgttt cagtttttac ttcaactgtc tcagctggtt cttctgttgt
      241 ttcaacagta gtcgttgcat catttgttac gacagtccct ggtttgaccg ttttgattgc
      301 tgcacgatcg tactcaagga agatcccttc gcagtctaaa acaactgtcc gtttgtcatt
      361 gtcgatttct gaaacaactc catgtaatcc gccgatcgtt acgactgaat cacctggttt
      421 catgctatct aacaagtttt gacgttcttg ctgttgtttc ttttgcgaac gtgacataaa
      481 aaaccacatt gctaatagcg cgataaacat cacaagcatt ggtaatgttc ccattttttt
      541 cacctcacta attctgtatc tacaaaaaac actttagcag aaaaatgctg ataacactag
      601 ttttttactt aatttttaaa agattttcct ttagaagctt tttgcgtttt ctttattgaa
      661 cccgtattct tcaaaaaagg cttctctaaa ttcaagcaaa ttatcatcca tgattgcttg
      721 acgtacttgt ttcattagat tcaacaagaa gtataaattg tggtaagaag tcaaacgaat
      781 cccaaatgtt tcatcacatt tgatcaaatg gcggatatag gcacgtgtat agtttttaca
      841 tgtataacaa tcacatttct catcaattgg acggaaatca tgggcatatt tcgcattttt
      901 cactactaga cggccttgag aagtcataca tgtgccattt cgagcgattc gagtaggcaa
      961 gacacagtca aacatgtcta ctccacggat aacaccatcg atcaatgaat cagcagcacc
     1021 gacacccatc aagtaacgtg gtttgttttc ggggatcaac ggtgtagtga attctaatac
     1081 acgattcatt tctgatttag gttccccgac agataacccg ccgattgagt aaccagggaa
     1141 atccatcccg actagatctt tcgcgctttg acgacgaaga tcttcaaacc ctgctccttg
     1201 gatgattcca aataatcctt gacggtctgg attagcatga gctttcaatc ctcgttctgc
     1261 ccatcgagac gtacgttcta ctgatttttt cacgtagtcg taactttcat cgaatggtgg
     1321 acattcgtca aaactcatca tgatatcaga gcccaattta ttttggatgt tgattgcttt
     1381 ttctggtgat aaaaacatac gcgaaccgtt caagtgattt ttgaaatgca cgccttcttc
     1441 ttcgatattc cgcatatcac ttaatgaaaa aacttgaaag ccgcctgaat ctgttaaaat
     1501 cggttgatcc cagttcataa atttatgcag tccgccagct tcttctacta agtcttcacc
     1561 agggcgtagc cataaatgat atgtattact taaaataacg ccagcaccca tttctttcaa
     1621 ctcttctggg gacattgttt ttactgtagc taatgttcct actggcatga acattggtgt
     1681 ggggaaagtt ccgtgtggcg tgatcaattc ccctaaacgc gcgccagtat gtttttcttt
     1741 cttgattaaa cgatagcgaa tggcaggttg cgtcattcat ttccctactt tctatataaa
     1801 atatcttgac aactcccacc atcataatcg tttcaagtgc tttttgcaag ttccctcggc
     1861 ttccttctac tttatttaat attctttttt ttatcctttt tttatgaata acaaaaaaaa
     1921 gaaaaacatt atgacagaaa tattttttga tacatggtat attggtgtta ttaaagaaaa
     1981 ggagggaaaa atcatgcaat cttctgcaga attaaaacgc caagcaaaag agagtctaaa
     2041 aggacgttgg ggacaagctg ttttactaaa tctaatccct acattgattg gtattgctac
     2101 tattcttctt attatgattc ctattatctt gtttgccacc accatatatg atggatcagc
     2161 gagcgtctca gaagcgacaa cttcaggagg cggtagttca ggaggcggtg gcggaattat
     2221 ttccagcatc attagcgctt tgttcatgag cggtatttct tggacttatc ttgatatttt
     2281 acgtggacaa aaagatcaga tcgaaccatt caaagatgca tttcgtggat tcaaaggttt
     2341 gtttatcggc ggagtgatca gcttagctgt gctgatctcg atctttacaa cattatgggg
     2401 tttgctatta gttattcctg gaatcattaa aagctattct tattctcaaa gctattttat
     2461 ttactacgat atcgtccaag aaacaggaca aacacctaaa gtactggata cgatcactgc
     2521 aagtcgaaaa ttgatggatg gttataaagg acaacttttc tggttagatc tttcatttat
     2581 cggctggcat atcttagcat tagcaactgc tggtattggt tatctatggc tcagcccata
     2641 catttctgca acaaaagcag cattttatga acaattgcca aaagatattt aaaagagagt
     2701 gtgacttgtg accgaggggg tggcaggaga acaccgttta tcgagaggca gtaaaagaaa
     2761 cattcagccc attagcaaaa aggaggtgtg acaacattca tgtcacacct cctttttgta
     2821 tagttccagt aaaaagaaaa tcaacttaga aacagttaat cattcctctt cttctgcttc
     2881 cggtaatcga tggaacttga tcagttcatt caccgcgatt ggtacgatag ataaaatccc
     2941 taccaagaac cagtgattca aactgatgtg tactaggccg aatagttctt gcgtaaatgg
     3001 cagcaaagca acagccgttg taatagctaa tgccagtaac gccatttcta ccaaagcttt
     3061 attggtcgct aaattgactt tgaagatcga ttggctgctg cgtacattga acacatgcag
     3121 aatcgatgaa taagctaaga tcaagaatgc aacagtttga ccgacatgct gacttgcgtc
     3181 tacccatggg ctgatagtat cgacataact acctaaataa taaccaaaca aggttacgat
     3241 ggtgaagacc cctgcattta tggcaatttt ttgcaataaa cctcgagcaa agatcccttc
     3301 atttttcgga atgggtggct gatccatgat gccgtgttcc gctggctctc ttcctaaagc
     3361 aaatcctgga agtccatcag caactacatt gacaaacagt agttgcacag ctgtaaatgg
     3421 tgctccccat cctaacagca tcgcaatcaa aacaataaag atctccgaaa tattacagct
     3481 tagcaaaaag ttgatagctt tacgaatatt ctgataaacc gaacgtcctt gactgacagc
     3541 gtctacgatc gtcgcaaagt tgtcatccgt caaaatcata tctgcggcac tttgcgccac
     3601 atctgttcct gtgatcccca tggcacaccc aacgttactt gctttcaacg ctggtgcatc
     3661 attcacacca tccccagtca tcgctacgac tgcacccgta cgttgccaag atttgacgat
     3721 ccggatcttg tcttctggcg ttacccgagc atagacagag agagctttga cacgcgcatc
     3781 taattcttcg tctgataatt gatgcagttc tgatcctgaa agtgcttctg acggatcatt
     3841 caagatacct aattctttcg ctatggcact ggcagtcacc acatgatctc cagtgatcat
     3901 gactgttttg atgccagcct ttttcgctcg cttgattgct cctttacttt ctggacgtgg
     3961 tggatcgatc attccgatca gaccaatcaa acgtaagtct ttttccagtg cttcagaaga
     4021 aatatcttgc ggttcttcgt cataagtagc ataaccaaca gcaatcactc tcagtgcccg
     4081 tttaccaaag cgatcattaa cgatcgccgc ttgttctaca tcgcctgaac taaagcgcgg
     4141 agcaagaaca tcgaatgctc ctttggtgat tgagatgtat ttttcgccca tttggtgaac
     4201 tgttgtcatc atttttcgtt cagaatcaaa aggaagctcg tttactcgtg gatatttttc
     4261 ttctaattct gcctttgtat gataattctc ttcaacagcc cttacgatgg ctacttctgt
     4321 tggattacct ttgataacaa ggtcgtcatc ttcttgctcg accgtcacat ctgtacatag
     4381 tgcagccatt ttcaatattt ccatcgcgct gttcgtcatt gaatcttcga tattcgttac
     4441 ttcatcttct ttgctccata cccggcggac gcgcatttca ttttgtgtca gcgttcctgt
     4501 tttgtctgag cagataacat tagcggttcc tagcgtttca actgctggca actgtcggat
     4561 gatcgcatgt ttcctcgcca tcttctgcac accgtatgca agtgtcaacg taacaataac
     4621 agtcaaggtt tccggtacag ctgcaacagc caaggaaatc gaagtcatga acatctcaag
     4681 gagtggttct ccttgcagtt caccaatcac aaatactaaa gccgcagccg ctaaagcgat
     4741 catactgatt cgtttgccta gctgattcaa tcggacctgt aaaggtgttt tttgcatagt
     4801 attttcgttc aaaagatccg cgatcttacc catttctgta gccatgccaa tggcagtgac
     4861 gatcgctttc ccacgacctg cagcaaccgt acatccttta aataccatat taagttggtc
     4921 acctaatgac tcatcttctt cagcaataaa tgctgcatct ttttcaactg cttcactttc
     4981 tccagtcaac gccgattctt ctacacgtaa ttgtgatgct tcgatcaaac gggcatcggc
     5041 agaaatagca tcaccggact ctaaaacaag aatatcaccc actaccagtt gctctgactc
     5101 aacagtggtc actttgccgt ctcgtatgac agttgccgtt tgtttattca tatcttgcaa
     5161 ggattccagt gctttttctg cgtttccttc ttggacaatc gccaataccg agttgatcac
     5221 aacgatagcg atgatcagca gtccttcaaa taaatcccca tgttctgtag caaatgcggt
     5281 ataaaaggaa atcaccgctg ctacaagaag gatgatcgtt gtaaaatcgg acaagcttcg
     5341 aaagaatttt ttgatcatcg attctttcgg cgcctcatcg aatttattca atccctgctt
     5401 ttttcgccgt tcatccactt cttgtgaaga caacccagta tctggatctg cttctgttac
     5461 cttctttact tcttctattg tctgcttgta ttcttccatc ctattcctcc tttaaaatct
     5521 tcgtacaaaa aaaggtactt attttactat aacatccttt ttttatttac ttcaaataaa
     5581 gggcttatcg attatttgtt aagaaagcgt aaaatttaaa gtgcttatat ttgcaaacgc
     5641 taccaaagga tgctattata tagatgtaag gaaggaggat accatcaaat agacagaaga
     5701 atttctcgat agaacagtgt aagcaaacaa agttgttaga agtacaggtt tcacccagac
     5761 atgcgtgtag agtacaaacc tataaatagt gaatgcgtat ggatgtttgg atcacgaagt
     5821 atccgaaaca agaagtaatg cgatacgtaa caattgaata acgtaaacgt ctgaaaattg
     5881 aataggaaca agagatgttt caaatgttga agatgtaacg aaaacaaaat ttttgtttaa
     5941 catgaagaaa ttcaacaccg tgctttgcat gtatgatgga aagttacaat catttctgtt
     6001 tatttgaatc cttccgaggt acaagaaagc cgagcttatc gagagctcgg ctttttcttt
     6061 gtcataaaat ctttgtaaag cttttttgtt attcaaaact agcggctttt cataacctta
     6121 tttcacgaac atcgcatctc caaaactaaa gaaacgatag cgctcttcaa tcgcatgctg
     6181 gtaagcagat aaagtcaatt ctcttcctgc aaaggcacta actaacatga ctaaagtcga
     6241 ttttggcaaa tggaaattag ttgagaaagc ttctaccact ttgaattcat agccaggcgt
     6301 aatgaaaata tcagtccatc cgctgtctgc tttgatttct ccattaaatt tcgttccaat
     6361 cgtttctaat gttcgaattg aagttgttcc gacagccacg attttcccgc catttttacg
     6421 tacttggttc aatcgctcag ccgcttcttc cgtcaaacgg tagaattcac tatgcatatg
     6481 gtgttcttca attttatcta cgctgacagg tcggaatgta cctaacccta catgtagtgt
     6541 cagatagact aattcaacac cctttgcttc gatctcatca agtaattctt tcgtaaaatg
     6601 caatccagct gttggcgctg cagcagaacc attttctttc gcatagacag tttgatagcg
     6661 atctgggtct tccagacgtt ctttgatata tggaggcaat ggcatttctc ccaatgattc
     6721 aagaatctct agaaaaattc cttcatactc aaagtcaatg atccgtccgc cgtgttccaa
     6781 ttcttctttc actactgctt gcaatctgcc atcaccaaaa gagattcttg tcccaacttt
     6841 ggcacgtttc gcaggtttga tcaatgtctc ccacgtatct ccttgtgtat tcgttagaag
     6901 aaggacttct aaatgcgcac cagtatctgg tttttctcca tataaacgag caggcagaac
     6961 tcgtgtatta ttcataacta gcgcatcacc aggctgtaac tcttcaatga tttcatggaa
     7021 atgtttatct tccatctctc cagttttccg atccaaaaca agtaaacgag aatgatcgcg
     7081 gtcttttaat ggtgtttgtg cgattaattc ttccggcaag tcaaaatcaa aatcttctgt
     7141 tgttaacata tcgtcactca ctttctatca tgtgcttcat cattctatca tgtttttcgt
     7201 tctgttggca aaatcagcac tttttcactt ctattttttg ttttcctaaa taaaaaaact
     7261 accctatttg tattttttca ttgttttagg catactagta atagatagtt cttcattgaa
     7321 aatagaaaaa gaggtggcac catgattgaa tttcaaaatg tgtcaaaaat ttataaaggt
     7381 ggaaaagtcg ctgtagagga cgtcaatctt tcatttgaaa agggtgagtt catttgtttt
     7441 atcggtacga gcggaagcgg aaaaacgacg acgatgcgga tgatcaaccg aatgactgat
     7501 ccaacacaag ggaaaatttt gattgatgga aaatccatcc aagaaattga tccagtggaa
     7561 ttgcgacgtc agatcggcta tgttatccaa agtattggat tgatgcctca catgacgatc
     7621 cgtgaaaata tcactcttgt ccaaaaattg ttgaaagtca gtcaagaaga acggaataaa
     7681 acagctgaaa aaatgattga tcttgtggaa ttgccccgtg agatgttaga tcgctaccca
     7741 catgaattat ctggtggtca gcagcagcgt attggagtcg ttcgtgccct tgcagcgaat
     7801 caagatatca tcttgatgga cgagccattc ggtgcgcttg atccaatcac tcgtgattcc
     7861 ttgcaagacc ttgtcaaaga tttacaagag cgtttgggaa aaacgatcgt atttgtaacc
     7921 catgacatgg atgaagcgat taaactagca agccgtatcg ccatcatgag tgaaggccgt
     7981 gtgatcaatt tgatacacca gaaaatattt tacgtcatcc tgcaaacgat ttcgtagaag
     8041 agttgattgg agaagatcgc ctactacaag ccaagccaaa tgcgacaaga gtcggcgaag
     8101 ttatgctaaa tacagcaatc acgattacac cggaaaaatc attacaagaa gcaatcaaac
     8161 tgatgaggga aaaacgtgta gatacgttgt tagttaccga taattcgaat gtattaaaag
     8221 gatttatcga tgtagaaacg ttgaataaaa aacgaggaaa agtatccagt gtcggcgaca
     8281 ttttgaataa acatgttttt tacgtaaaag aatccgccta tcttcgtgat accttgcaac
     8341 gcattttaaa acgcgggctg aaatacgtgc cggtcgttga tgaacagaac aaagtcgtag
     8401 gtatcctgac tcgtgcatca ttagtggata tcgtctacga tgtgatttgg ggagaagatc
     8461 ccgcaactac tgatgttgca gagtcagcta ctcctgaaga atctaaggag gtctgaccta
     8521 tgcagcagtt ttttcatcag cacggttcag aaattctctc aaaaggtttt gaacatcttt
     8581 atatttcagg acttgctttg atcattggta tcatcattgc tgtaccgctt ggtgtcttac
     8641 tgacacgttt tccaaaagct gccacgattg tgattggctt aaccagtgcc ttgcagacaa
     8701 tcccttctct tgctttattg gcattgatga tcccgctatt tggcgtaggt aaattacctg
     8761 cgattatcgc actctttatt tattcattat tgccgatttt acgtaatacg tacattggga
     8821 tgaaaaatgt cgattggaat tatcgggatg tggcaaaagg catggggatg actgagttcc
     8881 aatccatttt ttccgttgaa ttgcccattg ctatgccaac gatcatggct ggtattcgtt
     8941 tagctgccgt ttacgtgatt gcatgggcta cacttgcctc ttatatcggc gctggtggtt
     9001 taggcgattt gatttttagt ggactgaata attaccaacc agatctcatt ttggcaggta
     9061 cgatccctgt aacaatttta gcactattgg ctgattggct tctaggtctc ttggaacacc
     9121 ggctgacacc gatcgcactt agagaggaga atgaagaatg aaacgtatac taaaaatgac
     9181 catggttctt ttctctcttc ttatacttgc gagttgttcc ttccctggtc ttgccagcaa
     9241 tacagatgaa gacaccattt cgatcacagg tggaatcact tcagaagcac agatcttagc
     9301 aagtctagtt gccggaatgg ttgaacatta tacagataaa aataccgcaa tcatcaacaa
     9361 tctggctact acaactatca accatcaagc gatgatgaac ggagatgctt caatttcagc
     9421 ggctcgctac acaggaacag atttaacgac tacattgaac cttcctcctg aaaaagatcc
     9481 gaaaaaagcg tttgctaccg tgaaagatga atttgagaaa agatacggtc aaacgtggtt
     9541 cccgtcctat agttttgaaa atacgtatgt ctttcttgtt cgaaaagaca ccgcacaaaa
     9601 ataccatcta tctaaagtca gcgatctgaa aaatgtagcg gatgaattag tagctggtgt
     9661 agatacttct tggatcaatc gtaaaggaga cggttatgac ggcttccaag aaacttatgg
     9721 cttctccttc aattcgatat tacctatgca gattggactt gtttatgacg cagtcgaagc
     9781 aggaaaaatg gatattgttc ttggctactc tacggatgga cggatcgcca gttatgatct
     9841 agtcatgttg gaagatgatc gtcaattctt cccaccatat gatgctgctc cagttgtaga
     9901 caacaagata ttagctgata cgcctcattt aaaagaagcc ttaaataagt taggtcatac
     9961 catcagtacg gaaaaaatgc aggaattgaa ctacgaagca gataataatc tagtggaacc
    10021 atcagtagta gcacaacgtt tcttacaaga aaaccactat tttgaaggga agtgaagcaa
    10081 ttggataaaa tgaatgtctt tcaacaattt ttctattatt tccaggaaaa tggctcctat
    10141 atctttgcac aatttatccg tcacttcctg atttctatct atggtgtcct ctttgcagca
    10201 gttgtcggga ttccggttgg gatcatgatt tctaggagac gtaaattagc aaactgggtg
    10261 attcgtttgg cgaatatcat ccagacgatc ccttctctag ccatgatctc cattttaatc
    10321 attggattag gcctgggagt caatgtcgtg attgttactg tatttttgta ttccctctta
    10381 ccgatcatca agaatacgta taccgggatg atccaagtag acaaaaacat ccttgatgtc
    10441 ggcaaaggca tggggatgac tgctagacaa cgactattca tggtagagtt accactctct
    10501 gtttctgtta tcatggcagg cataagaaac gcgttagttg ttgccatcgg gataaccgcg
    10561 atcggtgcat tcgtcggtgc tggtggtctt ggagatatca ttatccgagg aaccaatgca
    10621 acagagggca catcgattat tttagcaggt gcattgccaa ctgcattaat ggcgatcatc
    10681 acggactggc ttttaggtat tttggaaaga cggctagatc cagccagccg tacatcacgc
    10741 tgatgttatt gtaaaaaaca aaagcttctg cgaccatttg ttggtcgcaa aggctttttt
    10801 agttctgtca tttttaaggc aataatccac tctgattctt ccctccttat gggcaaagag
    10861 ttgcttttct tagctattta tccattccgt atactccaac ttcatactgc caacgggttc
    10921 atatctgcat ccaccgacta agtttttttc ttatccgcta aggcattttg ccaatccatt
    10981 ttcccatcac tcgtcatgga attcatacca catgatctct aaatactcgt ctgcttgttc
    11041 tttgaattgg gcatcaattc ttacatcctt tggcgtggct ttcatttaag ccttttccat
    11101 cagtcctcga ttgttcgatg ccttcagctg catcatcaaa actggtgact gccgtatcgg
    11161 taacttcgat catcttttat acaatcggga acttgccatc ctcatccttg gctaaaaaca
    11221 tatctagcca ttccagcttc tcgccatggg aggtttgcgg ttcctaaaac cggtacacga
    11281 ttcttatgga ctgcatccgt cgcaactaga cttggcgata cgatgatcct tcaccagaag
    11341 agcacccccg taaaccaatt gatcgatata ttgccaataa gaaaagacat tggcgtaaaa
    11401 gatatcgatc cctcgtgatg cattgccgtt gatactgcta ttcatggccg aaagcgccac
    11461 gactttaact tcttccgttt gagtgacatt catgttcggc aatgtcgctt tggatacccg
    11521 ctctgctaaa ggaacgacac tggtattgta tttcgtatct gtatcttttc caaaagacca
    11581 tgccaacgaa tcctctggga accaataaga cgactccggt tgattgtcca ttgctttttt
    11641 caacatactt ttggctgttt ctcgataaac gacatttcca attgtcttcg cctcactttg
    11701 gcgataatcg atggtcgttg ccactgccaa cactaacaac gatgctccaa ttttactgat
    11761 tttccttgtc acacttcttc ctccttgttt tacataaacc cgtcaacatt ccattcctaa
    11821 actcaaacaa tgacccttca aaggtggaga tataggaaca aattaaagaa atcatgcaat
    11881 aaatccaaga aggttggttt tagaacaaaa aaagagtctc ctttttgggg agactctttg
    11941 ttaactatct aaaaaggttg gctcttctca cttcactcat aatgtgtcca ggcagaataa
    12001 atttctacaa ctttattttc ctcatcaact ttgtaaacca ctcgatgctg tctattcagc
    12061 ctgcgtgagt atctgccttc atgtgtaggt ctcagctttt caaaagactg cgtgggcata
    12121 tatggatctt ccttcaatgt ctgaatcact tcttcaaatt gtttctttaa attcgtttgc
    12181 ttgatttttc ttaaatccac tttcgcagag tttttgattg cgactgtata gttactcatc
    12241 aaagagcttc ccaatcaata tcatcgatat tcgtaaagct actgtcgtct ttttcgcggt
    12301 ctcgtacttt atctaaagta cctgtttgct caagaaaaag ggtttcttga atcgctctcc
    12361 agtcttccag cccaatcaat acagcattgt ttccactacg atcactaata atctcgattt
    12421 cagtatgatt ttcatttacc tctttcaata actgataaaa atttttacga gcagtgctag
    12481 gattcatcgt tgtattagcc attgtctctc ctcctttttc tgatataatt atagcgtact
    12541 tgattaagta cgtcaaaaaa atacaataaa gcacttaaat tcagcaaact tttcctcttt
    12601 tttacataga gaaaaagacc cctttttagg gaggatcttt gtccacgctc acttcttact
    12661 cctttacgac tagcggtagc caaacgtaaa gtaattggta acaacgtatc atctttagca
    12721 aaggtcgaat ccaaagcctg tgagtctatg caacaacacc aatacacgaa ggttcctcgt
    12781 tgcagtgtga cttgattgat atcttgaata acctgaggat acgcacgaaa gatttgtaac
    12841 ggttattcaa aactgatttt ggccgtcaag tcttctactg cctcaaaaat gattgcctct
    12901 ttcgtctcct gtcattcatg ttcactatcg atttgatact tgcttagacc gttgggtttg
    12961 tgaatcacca attaaaaatg gcactgagtt atggtaacaa aaaatggtca cttgacgagg
    13021 ctgagattcc tacttatgaa gacacatggc acttaagagt cgaggtaaag gaagaagctg
    13081 aaaatatagg aggctgtaca ttatttggca aaggattcgg aaattatgat caagacatca
    13141 aaatcaaaat gttttacagc taaataggaa gaataataac atttcatacg ctgttcccat
    13201 ctttaatggc ttatagaggg cacctttagc tgagaataag acaaaacaga ccctctagct
    13261 tcaattaaga gctagagggt ctgtttttga ttttaaaact tggtttattt cttcaaatgg
    13321 tagctatagg tgctgacaac gatattttca cgccaagaca atcgcaatct taaccgcaaa
    13381 cttgtttggt tatggagaat attctgccag cgtctgtttg gtacaaattg aggaatcaac
    13441 acagtagttg tatagttttg tttcgctgca tttttactta cgatatctac ataacggata
    13501 attggatttt caatcgagcg ataagaagag tggacgattg aaaaacggac atctgggaaa
    13561 tgctttttaa attctgcttc gatttctttt tctttttcga catcttcgtc tagcgaaaca
    13621 tgcatcgcaa cgacataatc accgatcgaa cgtgcgtaat tcaacgcacc gatatttacc
    13681 cgtgtcacat tcccgactaa tacaagaacg gtattcccgt cataatcatg tagttttgca
    13741 tcattttcta aacgaagctg ttctgctact tttttataat gatcatggat cttataaaaa
    13801 atgaacataa cgattggcat aatgatgaag aaaggccaaa tgtcccctaa ccgatagaca
    13861 aacaaaatcg caatgattgc atatgagatg aacgcacccg taatattggc aatcgatttg
    13921 cttaaccatc tttttccttc ttttttccat ttgacgacca tccctgtttg agataaagca
    13981 aatggaatga acacgccgat agagtataac ggaatcaagc gttctgttga gccatggaaa
    14041 ataaacagca atacgatcga acctagcgct agtgtgataa tcccattgga atagcctagt
    14101 cgatccccac gatcttgata catgtgaggc atgaatttat cttttgccag attgtatgca
    14161 agaactggaa aagcagaaaa accggtgtta gcagcaactg ctaaaatcag cgctgtagca
    14221 aattgaagga cgtaatacaa aataccatgt ccaaagacag ctttaccaat ttgagacaaa
    14281 acagtgactt ctttttcagg aacgatccca taccaatagt tgataaatgt gatccccacg
    14341 aagaagaaac ctaagatcaa tgccatcatc gtcaatgtag cagcggcatt ttttgctctt
    14401 ggcttcttaa agaatggtac agcattacta atcgcctcca ccccagttaa agaggaagaa
    14461 ccagaagaaa atgctcgtaa aatcaaagcg atagaaacac ccggcacagc cgctcctgga
    14521 agtgcagtag cttgcaatgg cacagcacct gtcacgatat tatataaacc aacaacgatc
    14581 agtaacgaga tcacaaggat aaatgtgtac accggaaata ataggaacga tgcagattcc
    14641 ctcagcccac gaagattcag catcatcagt agcaaaacga ttgtcacaga aatagcgact
    14701 tgatgaccgt agagtgctgg caccgctgat gtgatcgctt cagcacctgc tgagacagat
    14761 actgctactg tcaacatgta gtcgatcaat aaagagccac cggaaatcaa gccagcattc
    14821 tttccaagat tctcactgct gacgacatat gctcccccgc catgaggata agcatggatg
    14881 atttgacgat atgatagcgt taatgaaatc aataaaataa taacaaaagc ggcaatgggt
    14941 agcgaatacc agatggctgc agcagacaaa gcgactaaaa cgacaacgat ttgttccgta
    15001 ccatatgcaa tagatgataa cgcatcagac gacaataatg ccaaagcagc aaatcgggtt
    15061 aatttgtgct catcattttc agcagatttt aaaggcttac ctactaacaa cctttttaag
    15121 taatccaatt tctaaactcc tttacacaca attttacttc aagttttata gtcgaaaatt
    15181 tatagtaaac aagatgttat agtacgctca ctgaccttaa aagtcaataa actttaagaa
    15241 aataattgga aaaaaacaga aaaatatcaa gcgctttttc aaacgcttta atacagcaaa
    15301 aatctaacat ttttttatgg gctttttctt tgaattctat aataaaagga tagaacttga
    15361 cttagaaaat caagttctat cctttttgat atcttaactt ttggaagaga gatgtaaggc
    15421 tgctttctac ccatcattct tactactcga agcatagcgc agttgtcaca acctcttgat
    15481 aaacggtgtt taaaagcttg catctgcggt aataagccca accaaattaa aaacataaga
    15541 agctgttttt atttggtcgt tcttattact tgatgcagag cagctttttt acaacctctt
    15601 ggtacacggt gttcaatcag ctgaccctcg gtattagctc aaaaaaacgg caaaatatga
    15661 ggaactattt ttccattttt ttctctaata ctcaggtcaa aacactgatt tcacaacctc
    15721 tttttttaat gaaaaatatg gctctcatgc gccgcttctt tgaatttcgt cattttttct
    15781 tcgatgaacg gatagattgc cgttcctacg attccaaaga caacaaacaa accgatcatg
    15841 atccagattg ctttcgtagc atttggccaa taaattcctc ctgcagattc ccgtaacaag
    15901 tttaccgcat gagtaaatgg caagaacgga ttgatcattt ggaagaattt ccctgagact
    15961 tggatcggat agttcccgcc tccgccagag atcgacagta ctaagatgat aatagcgatc
    16021 cctttaccga cattatcgaa taatgcagcc aagacataga cgatcatcat gaacgctaag
    16081 gcgatcaaga cagcaaatga cacgctgtag accggatttt ggacatctac tcctagtaag
    16141 aaaatattcc ctaatgtaac gatcaagctt tgcgcaatcg ccattgtcaa aaatgtcagc
    16201 atacgagcaa cgaattgttc tcgtttcgta aaattgtttc ggtctttctt tcctaaatag
    16261 tattctgtcg tagcaacact tgaaagaaga acagcgccga cccacagaca tagtgcagta
    16321 taaaacgggg tgctagctga accgttattt gcgattggat aaagcgcatt ggtttgtagc
    16381 tcaacaggtt gtgtaaagaa gtcactttct tttgttgcgt ctagtttcaa taatttgatg
    16441 acttcgccta gatcgacttc ttgttcgcct tttcggatcg cgtctgctgc tttttggata
    16501 ccagctttga tatttggcca gtcattgttg atcaagtccg cagcagcgtt cacagctgtt
    16561 tcgacttctg gcattttttc atttactgtt gtcaatgtac cggtgatttc attttttagt
    16621 gctggccaat cattttggat aaaatcagcg gccaatccta atttttgctg aatgatcggt
    16681 aattcattgt tgtataggtc tgccccttca ttgatgccat tgacgatggt atccatattt
    16741 ccattcagca ttgtattggc atcatggatt tcttgtccaa ttgcaggcat ttctttttga
    16801 tatttttcta aaattgccac tgcattcgtt acagttgctt tcgttgagct gagaagcgaa
    16861 tcaaagtcga tctggtccgc tttgctcagt gcgccttgtg cagtcccaat cgtatcgatg
    16921 acttggtcta gtagagaagt gacagtggaa ctgatttgac ctgtattgat accagcaatg
    16981 atgccattga tattattaac aacggcatca atttgatcta gcaattcatc aatagccttc
    17041 gtgtcttcat tttgaacagc tgtgttcaaa gcagataggc ggttttgcag attgcttagc
    17101 aagggtttta attgatttag ctgatcaata attggctgta atttcgtatt tccagcactt
    17161 gtttgcaatt tagtcaggaa ctcgaccagt ttatcgattg cattttgttg ttgagatatt
    17221 ccgtcaataa agccgttcaa tacattgctg atgtttttct tttcatcatc agtcagctta
    17281 ccatcttcaa tagctgttct tacagtatct attaacgaac ctgtcgttgt tgccacttgt
    17341 ccaattgact tcaatgtaac atctacactg tttgtgatgc ttggtatcgc atctttcagc
    17401 tgagaggcac ctgattttgt ttggtttgct agatcacttg cctgattttc taaatttttc
    17461 acatctggca agatatcttg tacttgttgg atgatcgtca agccttgttt tgcttcatcg
    17521 attccttggt tcattgtctc ttggatcgaa gcaaaatcgc cgtcgatctc tgctagttgc
    17581 ttaccggcat tttggatctc tgggattttt tcttgcaaat caaggatgat ttttgcttga
    17641 tctttcaact caggcatttt gtcattcaat gcaacgactt tttcacccat ctcatctact
    17701 ttagggatgt aatccacgaa ttcatttgct ttattcagtt tttccttgat ttcaggtagt
    17761 tgactttgta attcgaggac ttgttttgtg tagccgtcga tcgtgtccaa attttcatct
    17821 gttgataaaa tcatatcttt aactttattg atacttacta ggttcgtatc gatgtcgtaa
    17881 cctatctcat tgaatacttt caataaagtc gaactggctg ttttgataaa ttcatttgtg
    17941 atttgcgatt ggatcgaact tgctccttta tcggtgattt tcggtgcaat cgcattgatt
    18001 ttttcattta ccgtatattc gatttttggt ttcttgatat caccactagt aaaactcagc
    18061 agatcttctg agaaatcctt tggcaagtaa atacctgcgt aatactttcc ggaacggact
    18121 ccgtcttcta attgttcttt tgaatcaacg aattgccatc ctaattgttt atttttatga
    18181 agcgattcga tgacttgctc cccaatggcc acttctttac cttgaaactc tgctggcttg
    18241 tctgcactgt aaacagcaat tggcagctct ccagtatttc catatggatc ccataatgct
    18301 ttgatgttaa accacgcata tagcgaaggg atgatcatga tcgcgatgat cagaagtgtt
    18361 gcaatcgggt ttttgaaaat ccgtttccag tcaagcaaat ataatcgaag cgtatttttg
    18421 attgactgca tagttctccc ccttcttctt ttttcagctg aattacttta ccatgtattt
    18481 catacttttt tcaatcggct tttttcaaaa aagtcgaatt tttggacata tcacggcaat
    18541 tgtctcattg taatctctta catattgaga aataattaag gattacagcc tttactttat
    18601 catgaaataa aagggaaaga taattttatg ccaaagtttt tatctattat acgtatacgc
    18661 aaaaaagcta aaacattttt tgtctttgcc cctacaaaaa tgtttcagct tttttcgttt
    18721 tttattcctg tatttcttat ttttcgaatg acaggatgat tttcccagca ggaggatgtc
    18781 cttcaactag ctgatgtgct tggatgacat gcttaagtga agcagacaga ttcattgaaa
    18841 taaaaacatg aaaggctcct ttttggtagg ctttgattat gccagcaaag gcttctgcat
    18901 ctagatattc ctttcttgga acaaacgatt cataaaagcc ttcctttttt tgccgcaaat
    18961 ctagatctgg gaggtcattt aatgcaacat acctgccgcc tggtttcatg atctggatgc
    19021 cggtttctcc tttgatactt cctttcgtcg catcgattac aaggtctgcc tgatcagcaa
    19081 attgcagacc tggatttgtt ttatcataag cagcaaatgc tgaagctcct aattttttta
    19141 ccttttcctc atttttactg gatgcactgg ttaagattcg gattcccttt tcatggagaa
    19201 gctggatcag acttgacccg accgcacctg atgctcctag tatcattaca gtatctgtcg
    19261 gctggatttc cgtcaaatgg ttgattagat tataagccgt aattcctggc gtgaccattc
    19321 cggctgcttc ttcccaactc attttatctg gaatttttgc tacttttgct gatggcaaca
    19381 cgacttcttc tccgtaagtc ccgccgactg catggacagc tactcgatca cctacatgaa
    19441 cagttgtgac atctgaacca atctctgtca caataccagc gccatcatta cctggtacat
    19501 aagggaattt caatgttcgt atctctttca tttctcctaa acgcaaggca acatcataag
    19561 gattcacact gaatgctttt atttctacac gaacatgtcc agaaccgact tctctgggat
    19621 gagcatcgat ttcttcaaaa acatcttgtg cttctccgta accgttaatt gcaaatcttc
    19681 gcatggtcac catctccctt tttctttaag tttacgttta tgcctttatt ttgacaaaaa
    19741 aaatgtcttt tcgttttggc gcatgattca aaacaaaata gttgtctttt ttcgcgtaac
    19801 gtgttaaatt atttatctaa aagcgtaaag attacgattt acgctaataa aaaataattg
    19861 gaggaatttg ttgatggcta aaaaatcaaa gattgccaaa gcaaaaaaac agcaagaaac
    19921 gattgaacgc tatgctgctt tacgccagga attaaaagcg caaaaagatt acgaaggctt
    19981 gcgtaaactt cctttagatg ctcgtccaga acgattgaaa aatcgtgatt taatcgatgg
    20041 acgccctcgc ggatacatgc gtaaattcgg gatgtcccgt ataaaattcc gtgaattagc
    20101 acataaaggt ttgattccag gagtaaaaaa agccagctgg taatccaagt ctggctgtaa
    20161 tgtaaaggag gagggcgcat ggaccactcc ttggatcatc aaaaaagata cgttcgcttc
    20221 ttctagcaga aatgctggta ctagaagcag acgtatcttt ttttacgcac agctggtcct
    20281 tttttatatg taaaggctgc atggttactt cgctatattt atttagtaac aggagtcagt
    20341 gcttcgatac ccacactaat tttcaatgct cggtcaactt ctgccataat gggtcgatcc
    20401 aaatggcaga ccttttcttt caatcttgat ttatcgatcg ttctgatctg ctccaacagg
    20461 ataacggagt ctctttctat tcctgttcca gtagctttga tcccaatgtg agtcggaagc
    20521 ttaggtttag ccatttttgc cgttatggcc gcaacaatga tcgtcgggct aaaatggtta
    20581 cctagattat tttgaataac caaaactggg cgtgttccgc cttgctccga tcccacgact
    20641 ggagaaagat cagcaaaata aatatctcct ctttttacca taggatcctc cttattcttg
    20701 atattctctt ggtatccgct gtcctaatcc gcaagccact tcatagtgga tcgtacctaa
    20761 ttgatctgct atatcctgca tagtgatttc tttgtctgca tttttgccga taagcgtcac
    20821 ttttgttccg acaggaaatt cttgaggcaa tcggatcatc agttgatcca tacatacacg
    20881 cccaatcgtt tcacagtagt tcccttctac caatagagaa aaaccctgca tctttcgtgg
    20941 ccagccatct gcataaccga tcggtatcgt tccgatccac tctgcttcag gagtgatata
    21001 tgtttctcca tagccgattc cttcacctgc tgggagtttt ttgacttgaa tcaattcaga
    21061 aaccagctca agtgctggct gcaatggata aacttccggt agtgcgtgcc cagagggatt
    21121 aagtccgtac attgctactc cataacgaat catattgccg attgtttcat catgccataa
    21181 agccgttgca ctattgctga catgtacata tcgaggaagt tcgggaagtt ttttcagcac
    21241 ttctataaag cgttcttttt gcagattcca atatgtatca tctgcctgat ctgctgtaga
    21301 aaaatgggta aataaacctt cccattctaa tacacgactt tctttgatga atgcagctgc
    21361 ctcttttact tcttcaggtg tacaaaatcc gatacgaccc atcccagtgt ctgcttttag
    21421 atgaatcgaa agaggcgtct ctagcttact tttctctaaa acagcagctg ctgcttgcaa
    21481 ccattcccgt gtacctgcag ttactgataa atcatgagca acagctacag tcagtgcgtc
    21541 gtaaggaacc atattcaaga taaggatggg ctgagtaaat ccagcttctc tcaactcgac
    21601 tccttcatct aaatttgaaa cacaaaagcc cgaagctcca cctgcaactg ctgcctcagc
    21661 tgtttcaata gctccatgac cgtatccgtt cgcttttact actgcaaaca attcttttcc
    21721 ttgcggaagt cttgcaactt catttgctac attttcagtt atagcttttt tatgtatcac
    21781 tgctttcgtt gggcggtgcc aagcgacaac catcgttttt ccttctttct agtcttccaa
    21841 aataatttgt gcaaaagctg tttcatttgt atgagtaata ctgacccata cattgccttc
    21901 atgaggtgaa cgcgtcacaa caggctgacc attttcattt tttaaaatct cgacatcttg
    21961 aaaggtaact tttccgatcc cagttcccca agctttagag aaagcctctt tgcaagcgta
    22021 acgcccgcct aaaaattcta cttggcgatg aaaaggcaag gactggaata gtttcatttc
    22081 atcagaggtc aagattcgtg caatgaattt tggtttttct tcgatcagcc gagtgatcct
    22141 aggaagttcg actgcatcga tcccaattcc ttttatcata tgcttttctc ttttcaataa
    22201 atttagaaca ctctcattct accaaattat aaccattttg ccacaaataa gtccacaaat
    22261 aacaaaa
//
LOCUS       DAZKLM010000049        22211 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3764
            SAMEA115706913-rid22582563.denovo.049, whole genome shotgun
            sequence.
ACCESSION   DAZKLM010000049 DAZKLM010000000
VERSION     DAZKLM010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706913
            Sequence Read Archive: ERR13196647
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22211)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22211)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287823.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287823.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,004
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,849
            CDSs (with protein)               :: 2,849
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 41 of 96
            Pseudo Genes (incomplete)         :: 52 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 15 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22211
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706913-rid22582563.denovo.049"
                     /isolate="VRE3764"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            281..2116
                     /gene="tdc"
                     /locus_tag="ACG842_001640"
     CDS             281..2116
                     /gene="tdc"
                     /locus_tag="ACG842_001640"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004837 - tyrosine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HFQ0248185.1"
                     /translation="MKDMDIKAVFIGDKAENGPVYKMLLNKMVDEHLGWRENYIPSDM
                     PAISEGDKLTPDYLATRDHMIEVLDEVSQRLRAGSIPWHSAGRYWGQMNAETLMPALL
                     AYNYAMLWNPNNVALESSMATSQMEAEVGQDFASLFNMTDGWGHIAADGSIANLEGLW
                     YARCIKSIPLAVKEVLPEKVKKMSEWELLNLSVEEILEMTESFTDEEMDEVKAASSRS
                     GKNIQRLGKWLVPQTKHYSWMKALDICGVGLDQMVAIPVQEDYRMDINALEKTIRELA
                     GQKIPILGVVAVVGTTEEGQVDSVDKIVQLRERLKDEGIYFYLHVDAAYGGYARSLFL
                     NEAGEFVPYASLAEFFEEHHVFHHCVTIDKEVYEGFRAISEADSVTIDPHKMGYVPYA
                     AGGIVIKHKNMRNIISYFAPYVFEKSVKAPDMLGAYILEGSKAGATAAAVWTAHRVLP
                     LNVTGYGQLIGASIEAAQRFREFLDHLTFTVKGKTIEVYPLNHPDFNMVNWVFKEQGC
                     TDLNAINELNEKMFDRSSYMDGDVYGERFITSHTTFTQEDYGDSPIRFVERMGLTKEE
                     WKKEQKITLLRAAIMTPYLNDDRIFNFYTKKIAKAMEKKLNEIIQ"
     gene            2421..3899
                     /locus_tag="ACG842_001641"
     CDS             2421..3899
                     /locus_tag="ACG842_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFQ0248186.1"
                     /translation="MKDTGSSKQITTMTFIGMTCALVASVRNIPDVAATSWTMIFYML
                     VAVLLYAFPISLISGEFAGMFPQKGGPELWVSNALGKKWGFVVSWLLWVQMFPGMVMV
                     ASALAPLFGNIIDNVPLGLNSKFTLVVILVVYWIITFLNLKFDMAKIGGKVGVWLGLY
                     VPLTMMLLLGFAAWIKVGIVPTGTLGSFSWDKLIPDTTTASSFVYFAPIMFIFTGIEM
                     SSVYITRLENPVKTYIRGVFAALIFIFFVNILNALVVANVVPKGQMELNNIAQSISIY
                     CQILGLPHLIVNLFSLLVFIGVAVQLSAWASGPAKTVTESARKGAYPPKFNFWKTNQF
                     DVSKSVILTQSVIISIFALFYLLIPGVNQAFLMLVNSTTVIYCIVYVIMGIAVLRLRY
                     THAKLNRPFRIGKKEKKSNLGVWTVVIVLFAAIGFSVGLTMKAGTWINLIAVTAISVI
                     LFVVPLCIEKIKKPSWEQEVAMLLEKEKGELDGKTGNETDSN"
     gene            3865..5196
                     /locus_tag="ACG842_001642"
     CDS             3865..5196
                     /locus_tag="ACG842_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFQ0248187.1"
                     /translation="MEKPAMKRTLTNRNIQLIALGGAIGTGLFLGSAKAIASAGPAVI
                     LVYLICGLAIYGLMRAMGDLFLANSTFNSISDFVDYYLGKKWAFFVNWTYWLCWLGAG
                     LAELTAIGLYIHFWFVHVPALISGGSALMILMAVNLVAVKWFGELESFFSIIKILGIF
                     LFILLGIGLSVSDFHTQSIGIKENFGQIDELLPFGFAGMVVSFPMVLFSFAGIEMIGL
                     TVGETSDPQKNLKKAINCLPWRILFFYIGTILSILFVVPWHCLDLKESPLVTMLQVIH
                     CQGGAAIVNLMILVASLSSANSAVYSTSRILYQTAKEKSLPHWFCALSKKGVPLHGIL
                     ITGMLFFAGLCLHFFVADSRLFFQLLAGMTTSCFLFVWSSILCAHVQFIKEKKRNTTQ
                     RYKDLALLVFFTSIAFSLLFESTTRFIPFLLVIWFVFLNFVYKRISNRNIS"
     gene            5310..7862
                     /locus_tag="ACG842_001643"
     CDS             5310..7862
                     /locus_tag="ACG842_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HFQ0248188.1"
                     /translation="MNWYRLTVEDVLKNYQTSTEGLSQEERQKRLTDQGYNEIKARQQ
                     TKRWKKIAKHFTDLLMIVLIIASFLKFASSEYIEGSIILFVVIVNGLVSYWQERKAEE
                     SLNGLKQLMGQEAIVLSNGMQEKIPAKELVKGDVIVLRAGDVVPADIRLIEAHDLLVE
                     ESILTGESEASEKSHAVLTEEESIGDQKNMGFSGTLVQSGSAVGVVVETGNTTEIGKI
                     NQALQSVEQQTTPLIKKINRLNKQIFQGILCLSLFLVIFTTFRYGMDWHILLSATIAL
                     VVSMVPEGLPAVLTMILSVGVHEMAKEKAIIKGLPSVETLGSMTVICSDKTGTLTKNE
                     MTVVDVAAKEEACVLSIMKNCQELKTKEEQKTENLSGNPTEVALIRHTETANLPLKKI
                     EAKIPFSSEYKYMATMHAEEKGAVIYVKGAPEVLFAKSTLSSAEQEAWSQTAAEFARK
                     GQRVLGFAYKKVDSKQELTHETLTQLTFAGIAGLIDPPKESAVKAVKECQQAGISVKM
                     ITGDHKDTAKAIAEQIGLKHTAKVLEGIDLDLMSDEELIQQVPIVDVFARTTPEHKLR
                     IVKALQKNGEIVGMTGDGVNDAPALKRSDVGIAMGIKGSEVSKQAADMVLGDDNFHTI
                     AKAVKEGRRILDNLQKTINFFLPTALAQGLILIWALMLNRPLPLSPVQILWVNMVTTI
                     TLSYALGFESPDPEIMKRPPRDPKQGILSGYHLFRIIYVSLLIMVPAYLLAMQFEGQS
                     LQQTILLQNIVLAQAVYMINCRELSKPSLNQGLLQNKALFLSLGILALLQGMVIFLPV
                     GQQLIGTVSLTMTQQLLIGANVILLFLVVEIEKGIMNKLFRKKEKTAREYSR"
     gene            complement(7869..9308)
                     /locus_tag="ACG842_001644"
     CDS             complement(7869..9308)
                     /locus_tag="ACG842_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0248189.1"
                     /translation="MYSLMKKIITEKDIRRHVSLVEQLLNHTKITVNELAEIIGTTER
                     TIFSDLQSIRSQLPEGWDIISDQAGISLQNQQNLLTNDLWEIFFKQSVSVELLKNLLF
                     TKKVAVPDFLADHGLSYGTLKRHVTKINQRLASYDLQIDLTKYTACILGKERAIRTFY
                     HRLLIPFTHNNYFFEDYSIHESHYFQFLRNLSQTELAVETEEIFGTCWFFINTIRIKA
                     NCRLDSSIHTNSTLSSLYDSALKKLYLTEGIYLKDTELSFASFCFLESWNYNNNYGQE
                     TARCLHHSPFLEVLETFVEELASELSLDQLKKTSLTDNLALLILKYHESPILIEALDR
                     QYHRFLETYDQQYAHLYQQKENLLNDLKKEIEINEPNYFLQLLSLLIQQTIFSIKPNL
                     FNVYFIFQGEPSWKAFLQQELKDYLGKRVCFLPIELTDLSTISFEKTDILISNFPLDS
                     LDIPIVYISSIPTKNELNRLTELTFKTFL"
     gene            9283..9465
                     /locus_tag="ACG842_001645"
     CDS             9283..9465
                     /locus_tag="ACG842_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0248190.1"
                     /translation="MIFFISEYINQTPFYSKNTEKINGGQVSTDFSIIVYFSGAFTDD
                     KNFIMNLKNDTTKIKK"
     gene            9654..10016
                     /locus_tag="ACG842_001646"
     CDS             9654..10016
                     /locus_tag="ACG842_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HFQ0248191.1"
                     /translation="MLTKKELIDHINETYNLSPEYIFKKFPDYCVFRHQKSKKWFGVL
                     MSVPEEKIYDDASDHRIINILDLKVDPELGGILRGKEGYFPAYHMNKEHWISIDLRKI
                     EKLDELADLIDGSFKSTQ"
     gene            complement(10203..11060)
                     /locus_tag="ACG842_001647"
     CDS             complement(10203..11060)
                     /locus_tag="ACG842_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFQ0248192.1"
                     /translation="MKLLHLQYFKKVFETKNVTQAAKELFISQPALSRAIKHLENELG
                     VPLFYHSGRNIETTVYAEEFYPYAVKTLDTLEEGIKLLSSVNEKIVTSVILYLEVASV
                     SIPNLVRIFMEKHPDIQLTIMQHHLPDDITDPVLYITSEMKPGMTNIPIMKEPVYVAI
                     PKKHPLAKKEHLQLEDIQRTPILMLSKSNAFRHTLDAALEAKSIELTISSTTDDPATL
                     RSIVKQGLAVSFFPKVSWSYDKNDPFVLREIKDLPLTRTIYLSSSFTEDHPLIKMIAK
                     TMREFYLGK"
     gene            11180..12091
                     /locus_tag="ACG842_001648"
     CDS             11180..12091
                     /locus_tag="ACG842_001648"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HFQ0248193.1"
                     /translation="MSTGFLFPGQGSQKLGMLADIPEMYMERLENVTGYRLVDKEENY
                     QDTVFIQLALLTKAVFYLDEMEKNGIQPDIAAGHSIGAFCAAVACRSLRFENAAELVY
                     HRAVLMKEAYPSGYAMGVVVGVTRSEAEEIVAKTFDPDHPVYLSNENCPMQHTLSGSI
                     EGLSVTLAEAKRHQAQTAKLLKVPVPSHCLLMNDTVKKFKPYMQSVHLSDARCLYIKN
                     TDGRATQDAEEIRKDLLENLAFPVQWNQMMDLAKELGMDLTIEFPPGNTLTKLIHTKF
                     GVDKGIRTINLDQHGIDDATFLYQKWR"
     gene            12098..13744
                     /gene="mdcA"
                     /locus_tag="ACG842_001649"
     CDS             12098..13744
                     /gene="mdcA"
                     /locus_tag="ACG842_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015451 - decarboxylation-driven active
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit alpha"
                     /protein_id="HFQ0248194.1"
                     /translation="MEKPNRNWASKREKKAQRLEKISNLLNGKYVETEKIVEVMEKLI
                     APGDKVVLEGDNQKQASFLSESLEKVDAEKVNDLHLIMSSISRPEHLNIFEKGIASKI
                     DFSYAGAQSLRVAQMIEDGTMKLGDIHTYLELYGRLFIDLIPNVVLVAADKADKDGNL
                     YTGFNTEETPTIIEAAAFKDGIVIVQVNEVVDSLPRVDIPGDWVDVVVKADKPYQLEA
                     LFTRDPQNITELQILMAMMAIKGIYAEHGVQSLNHGIGFNTAAIELLLPTYGESLGLK
                     GKIAKNWVLNPHPTMIPAIESGWVESIHSFGGEVGMEKYIAARPDVFFVGKDGTMRSN
                     RTLSQAAGQYAIDMFIGSTLQLDYEGNSSTVTKGRLSGFGGAPNMGHNPGGRRHSTPA
                     WQSLRDNDDPLGKGQKLVVQMVETYGSNKKPVFVEELDALAVQKQAGLANAPVMIYSE
                     DTTHIVTEEGIAYLYKAKTKEERQAAIAAIGGVTPLGMTSTKESLDSLRAAGIVKLPE
                     DLGIKRSEAKRSLLAAQTIDDLIEWSDGLYEPPMKFRTWS"
     gene            13846..14145
                     /locus_tag="ACG842_001650"
     CDS             13846..14145
                     /locus_tag="ACG842_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit delta"
                     /protein_id="HFQ0248195.1"
                     /translation="MEQLAYKYPTKQPINKKIHVGVVGSGDLEILMFPTEKTESTVKI
                     CTGSDGFGEVWNNVLTRFFDRYPISADIVINDFGATPGVVYLRLTQAMEELSDEK"
     gene            14135..15811
                     /gene="mdcD"
                     /locus_tag="ACG842_001651"
     CDS             14135..15811
                     /gene="mdcD"
                     /locus_tag="ACG842_001651"
                     /EC_number="4.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-independent malonate decarboxylase
                     subunit beta"
                     /protein_id="HFQ0248196.1"
                     /translation="MKNSFVELNARERVNALLDNGRELIDPFDQIIAPSLPAQGIVPE
                     SDDGIVVSRGTINGKQAVVIAMEGKFQGGGIGEVSGAKIVAALEHVLEENKKGNEIFP
                     VIVLDTGGVRLQEANYGLLSISEIGNVIIALKQYVPVIGLIPGRVGSFGGMSITSALM
                     SYLIATKKARVGLNGPEVIEQEAGVREFDSSDKDLIWNTLGSRQRVESKIIDELVTDS
                     VEAIKAAVIAAMDERKDTHRSESSAFYLSLLDSLDLSKPMAIDDYNEAIANHQAKSID
                     LPEVAEGNEPAAASVGYDWFAALTGMKNPTSSISTVYYGKSSKFIEEAIVTSIVPDPK
                     NPMYRVRNGEVGLVEGFRMGQILDHVYEEDKDKKVKRPIIVVIDVPSQAYGYNEELIG
                     IHVALANSAAAYAKLRQAGHPVIGLIAGNAISGAFLAHGLQSSRLIALNNKEITVQAM
                     SKESAARVTKRSVAEIEAAAEKVPAIAYDINNYTKLGAIYHFLEDITDQAASEQNVSK
                     VIEAINEAIQDVRNTNDTMLTNRYTNETAKTFGRVETNKVRAKMDEEWDA"
     gene            15815..16408
                     /locus_tag="ACG842_001652"
     CDS             15815..16408
                     /locus_tag="ACG842_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase holo-ACP synthase"
                     /protein_id="HFQ0248197.1"
                     /translation="MIQAHDIVLFDKHEIEGFTLPEWVVLTEKMFGTVRRASTDHAEI
                     LPIGLRGPARNQRWSTEISKHAVKQVIHPWDIIEQESYRQEKIMDYPVYQQFHAARNI
                     LSGCKWGIGGSLGFELSTGIPAVKETSDFDLLLYANSPIELPIQVIQSHPAFFEQFDT
                     QVVTRQGGFSLKEYLREPEKKILLKTVEGPKLTKELW"
     gene            16432..17508
                     /locus_tag="ACG842_001653"
     CDS             16432..17508
                     /locus_tag="ACG842_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HFQ0248198.1"
                     /translation="MFENKKIKINVSDFQKVSAVTAVMMQTILSFALANSHSATNTAF
                     LGTFYVIVKYTAPMFIFAIVYNMVKTSQHLSYWEFLKEKFFELVVPYLLWTTAYLWLF
                     PAVQQQTPYTDTASFLLKYVTGDGAPHLWYTVMMLQIQLFMPFFVWLGYKVFANKKYV
                     WPVLIGSTILYVAWYLFYQTQVLTGPYHDSWYLLDRFVASFMIYGVAAFVYHEKVYQY
                     LDRVRYLFLPVALVIAFFSVRSLLAHPGDLSFANAPYLNTIQSLYSLVIIFAVFIGAS
                     KMIVNDSPKLPLFKWLSVYAYRTYLANVFVFQVLLLLFKDSWLQLPSGVMILVAYLMT
                     ASCAFALSWLLHIIWVAIKKGFSK"
     gene            17533..18402
                     /gene="mdcB"
                     /locus_tag="ACG842_001654"
     CDS             17533..18402
                     /gene="mdcB"
                     /locus_tag="ACG842_001654"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046917 - triphosphoribosyl-dephospho-CoA
                     synthase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA];
                     GO_process: GO:0019361 -
                     2'-(5''-triphosphoribosyl)-3'-dephospho-CoA biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase MdcB"
                     /protein_id="HFQ0248199.1"
                     /translation="MINDERKKAVTQQLAALVEEALIAEVTLSPKPGLVDALDAGAHS
                     DMDYALFLKSAKTLTPFFEEMAQIAWHHAIDQELRERIAEIGRKAEKAMLIATNGINT
                     HKGAIWAMGLLISVIAQKISMEQPFSLSKILDDVSKLAAFPDTKYRHKKTTHGHRVKQ
                     KYGVNGAYEEAILGYPHIRLAIQAAKELDNDSYVQKQMHMLLVLMSSLDDTCVLHRSD
                     QKTLVEMQKLAHAASQSALPNRPFTALLHFCQENQISPGGSADLLSASLFMLKIEELW
                     TADTYICPMLLND"
     gene            18513..19277
                     /locus_tag="ACG842_001655"
     CDS             18513..19277
                     /locus_tag="ACG842_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM44-like domain-containing protein"
                     /protein_id="HFQ0248200.1"
                     /translation="MKKINYLAIILLVGLCLFALPTQGFAVAGGHGGGTSSGRSSGRG
                     SFGGRSYGGSYFGSRSYGRDSYGYYGPGTGPSSYEQTGFLALAVLSVGGVSAFSIVKN
                     KRQKKRLKAEILSRMSGTAKEKKAQFLEIQKIFMKIQSAWEEETPEKARSCYTDKLFA
                     EHQKVLQKNKADGVRNHTKKITIQEVGNYRQIHENSFAIRIDFRCCDYSVDRKDGRLI
                     SGSKTQRQYFSQIWYFDFSEKEKRWQADFIQPIMLD"
     gene            19766..21859
                     /locus_tag="ACG842_001656"
     CDS             19766..21859
                     /locus_tag="ACG842_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFQ0248201.1"
                     /translation="MKTRTIEILHRIMTAQYPVKAKHLASEFNVSQRTIRQEVLEANI
                     WLSSKKLPEIRTIRNKGFLLKLEKEKQARLETEIKDVKDGFLNRRERIFDLVLAVAYS
                     RIPIHLNKKEEEFQISKSTLDEDMRRLRATLVSYGIEIVSYGKQGLVYKGSERSIRTM
                     IYDFINQNLGMTDFFMNQDAQLTPAQKIFLHYFPLVEIKKIQEIYSNKLLKQEDDIYK
                     KQILLFTLIWIQRIRKHEFISAINWKNTEIKDSSFSAFIDHMIEEFQLDNVPQVERNY
                     LRFTIETFNARDISNSIEWVQAQLLTIQLIQFVENETHVPFHLKEEALFENLYKHMAA
                     LIVRIKNHIQVMNPLKENIRTNYFPIYRAVTHFVPTIEEVIGGKILEDEIAFLVIHFS
                     TIASVIKRDLNYVYKSVVVCNHGMATGNLLAENLKEKFPQIEITAVLSSKEIELVDKL
                     DVNLVFSTFQLNYRNKPLLVVDPILTETNILVIEDFLKRNEELQRLEPNSHESTELFT
                     QIIDVMKESGGRVDRIIYGKLEKLFETNNLEINKREIQPMLKDILTDSNIMIKEKATT
                     WEESIELAARPLVKENIVEERYVEAMIRAVNDYGPYIVIGKHMALAHARPEDGVNKLG
                     VSVATIEQPIDFGNPEMDPVKIIFCLAAVDSYSHLNIMKELIELINNEEKLDRLIECT
                     NIEDFKQLLFSVVEK"
     gene            21884..22186
                     /locus_tag="ACG842_001657"
     CDS             21884..22186
                     /locus_tag="ACG842_001657"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFQ0248202.1"
                     /translation="MNKLNILFVCGAGLGSSFAAQMSAEDVLNKLGVEAKLDHCDISS
                     AVSMKPDVIITAENFRSQFEKFAVDPKTAMIYLKNIVSKNEIEEKITPVLQDKGVI"
BASE COUNT         7195 a         3789 c         4730 g         6497 t
ORIGIN      
        1 aataaaaata tgtttagtga atgaaggaca gtcattttat ttgtttgttc gcttttttta
       61 ggttatctat tttagaagac tgatttattg aaaaattgtt cgcttttttg cgttgaatta
      121 tcagaaaaat ccgactttat ttatttttgt ttgtaaaaat ctacgtggat ttgatgttat
      181 aaaaaaaaaa ttctcatttt tggcgctata gtgaagaggt gttacagata aacacatgta
      241 tctgcaagac attgaccaac aaaatatgga gggaagaaaa atgaaagata tggatatcaa
      301 ggccgtcttt ataggcgaca aagctgaaaa tggaccagtt tataaaatgt tgttgaacaa
      361 gatggtagat gaacatcttg gatggagaga aaattatatt ccttcagata tgcctgcaat
      421 tagcgaagga gataagctca ctccagatta tctagccacc cgcgaccata tgatagaggt
      481 cttggatgaa gtcagtcagc gattacgcgc aggatcgatc ccatggcatt cagcaggccg
      541 ttactgggga caaatgaatg ctgaaacatt gatgcctgct ttattagctt acaactatgc
      601 gatgctgtgg aacccaaata atgttgcatt ggaatcttct atggcaactt cgcaaatgga
      661 agcagaagta ggacaggatt ttgcttcatt attcaatatg acagacggat ggggacacat
      721 tgcagcagat ggttctattg ctaatcttga aggattatgg tacgcccgtt gcatcaaatc
      781 gattccttta gctgtcaagg aagtgctgcc tgaaaaagtg aaaaaaatgt ctgagtggga
      841 acttttgaat ttatctgttg aagaaatttt ggaaatgacc gaaagcttta cggacgaaga
      901 aatggatgag gtcaaagcag cttcttcccg tagcggaaaa aatatccaaa gattagggaa
      961 atggctggtc cctcaaacaa aacactattc ttggatgaag gcgttagata tttgcggagt
     1021 cggcttagat cagatggttg ctatcccagt tcaagaggat tatcgaatgg atattaatgc
     1081 tttggaaaaa acaattcgtg aattagctgg acaaaaaatc ccaattctag gtgtggttgc
     1141 tgtcgtagga acgacagaag aaggtcaagt agacagtgta gataaaatcg tccaattaag
     1201 agaaaggtta aaagatgaag ggatctattt ctatttacat gttgatgccg catatggcgg
     1261 ctatgcgcgt tcactgtttc taaacgaagc aggagaattt gtgccgtatg cttccttagc
     1321 tgaattcttt gaagaacatc atgttttcca ccactgcgta acgattgaca aagaggtata
     1381 tgaagggttc agagctattt cagaagcgga ttctgtcacg atagatcctc ataagatggg
     1441 ctatgtccct tatgctgctg gaggaatcgt aatcaaacat aaaaatatga gaaatatcat
     1501 ttcttatttc gcgccatatg tgtttgaaaa atcagtaaag gctccagata tgttaggagc
     1561 ttatattttg gaaggatcaa aagctggagc aaccgccgcc gcagtctgga cagcacatcg
     1621 tgtattgcca ttgaatgtga ccggatatgg tcaattgatt ggggcttcaa tcgaggcagc
     1681 tcaaagattc agagaatttc tcgatcatct gacttttact gttaaaggaa aaacaatcga
     1741 agtctatcca ttgaatcatc ctgattttaa tatggtcaat tgggtattca aagaacaagg
     1801 ctgtacagat ttgaacgcta tcaatgaatt aaatgaaaaa atgttcgatc ggtcttctta
     1861 tatggatggc gatgtttatg gtgaacggtt tattacttct catactacat ttacacaaga
     1921 agattacggt gattctccga ttcgttttgt tgaaagaatg ggtctaacga aagaagaatg
     1981 gaaaaaagaa cagaaaatca ctctgctacg tgcggctatc atgactcctt atttgaatga
     2041 tgatcgaatt ttcaattttt atacaaaaaa aatcgccaaa gcaatggaaa agaaactaaa
     2101 cgaaatcatc caatagaact gatttttgtg tgaaagaata acaagagaca tatggagaac
     2161 agacaattat ttgcttcatc aaaacaattg tatttccccc tgtttttcat atattcctcg
     2221 ccaagatatt cttttttata aaacgttcta taagttggat cgttcgtttc atgtatcaaa
     2281 cccagttcac taagatcgtt caatggatgt ttcggtgata ctgggttttg tactgaatcg
     2341 atgaaacctt gtcatttctg ttcttgaaca aaaataaaag aaaacaaaaa catatgtcgc
     2401 tattgaaaga aggaataaag atgaaagata ctggttcatc aaagcaaatc acgaccatga
     2461 cctttatcgg aatgacctgc gcccttgtag caagtgtacg aaatattcca gatgtagcag
     2521 caacgagctg gacgatgatt ttttacatgc tggttgcagt attattgtac gcttttccaa
     2581 tctcattgat ctctggtgaa tttgctggaa tgtttcccca aaaaggtggt ccagaacttt
     2641 gggtatcgaa tgctctagga aaaaaatggg ggttcgttgt ctcatggctg ttatgggtcc
     2701 aaatgtttcc tgggatggtg atggtcgctt cagcactcgc tccgttgttt ggtaatatta
     2761 tcgataatgt gccgcttggt ttgaacagca aatttacatt agttgtcatt ttagtcgttt
     2821 attggatcat cacttttttg aatttgaaat ttgatatggc aaaaatcggc ggaaaagtcg
     2881 gtgtttggtt aggattgtac gtgcctttga cgatgatgct cttattaggg tttgctgcgt
     2941 ggatcaaagt tgggatcgta ccaaccggaa cattaggcag tttttcatgg gacaaattaa
     3001 taccagatac tacgactgca tcctcatttg tttattttgc gccgatcatg tttattttta
     3061 ctggtatcga aatgtcctct gtgtatatca ctcgattgga aaatccagtt aaaacgtata
     3121 ttcgcggagt atttgccgcg ttaattttta tatttttcgt caatattttg aatgctttag
     3181 ttgtagcaaa tgtcgtacca aaaggacaaa tggaattgaa taatatcgcg caatctattt
     3241 cgatttattg ccaaattctt ggtttgccac atcttattgt gaatctgttt agtttacttg
     3301 tcttcattgg tgtagcagtc caattatctg cttgggcctc aggaccagca aagacagtga
     3361 cagaaagtgc tagaaaaggt gcgtatccac caaaattcaa tttttggaag acgaatcaat
     3421 ttgacgtatc caaatccgtt attttgactc agtctgtcat tatctcaatc tttgcacttt
     3481 tttatttgct gattcctggc gtcaatcaag catttttgat gctcgtaaat tcaactactg
     3541 ttatctactg tatcgtctat gtcatcatgg ggatcgcagt tttgagattg cgttatacac
     3601 atgcaaagtt gaatcgacca tttagaattg gaaaaaaaga gaaaaagagc aatcttggag
     3661 tttggactgt cgttattgtt ttatttgcgg caatcggatt ttctgtcgga ctgacgatga
     3721 aagctggaac atggatcaat ctaattgcag tcacagccat cagtgtcatt ttgtttgtag
     3781 taccactctg tattgaaaaa ataaagaaac cttcttggga acaggaagtc gccatgctgc
     3841 ttgaaaaaga aaaaggtgaa cttgatggaa aaaccggcaa tgaaacggac tctaactaat
     3901 cgaaatatcc agttaatcgc attaggtggc gcaattggaa caggattatt tttaggttca
     3961 gcaaaggcaa ttgcatcagc agggccagct gttattctcg tttatttgat atgcggtttg
     4021 gctatttatg gtttgatgcg ggccatgggt gatttatttt tagcaaatag tacatttaac
     4081 tctatcagtg atttcgttga ctattattta ggcaaaaagt gggcattttt tgtcaattgg
     4141 acttattggc tttgctggct tggagctgga cttgcggaat taacagcaat aggattgtat
     4201 atccatttct ggtttgtaca tgtaccagct ttgataagcg gaggtagcgc actaatgatc
     4261 ttaatggcag tcaaccttgt tgcagtcaag tggtttggtg aattggaatc ttttttctca
     4321 atcatcaaga tcttggggat tttccttttt atcttattgg gtataggatt gagtgtctca
     4381 gattttcata cccagtctat cggaatcaaa gaaaattttg gacagatcga tgaattacta
     4441 ccgtttggct ttgctggaat ggtagtctca tttcctatgg ttttattcag ttttgctgga
     4501 attgaaatga tcggcttaac cgttggcgag acaagtgatc cgcaaaagaa tttgaaaaaa
     4561 gcaatcaact gtctgccttg gcgtattctc tttttctata taggaacgat tttgtcgatt
     4621 ttatttgttg ttccatggca ttgtttagat ttaaaagaaa gtcctttagt gactatgctg
     4681 caagtgatcc attgccaagg cggagcagca attgtcaatc taatgatttt ggttgcttct
     4741 ttgtcctcag caaatagtgc agtctacagt actagcagga ttttatatca aacagcaaaa
     4801 gaaaaaagtt tgccccattg gttttgtgcg ctttctaaga aaggcgtacc tttacacggt
     4861 attttaatta caggaatgtt attttttgcc ggtctttgtc tacatttttt tgtagctgat
     4921 agtcgattgt tttttcagct tttggcaggc atgacaacga gttgttttct atttgtgtgg
     4981 tcttcgatct tgtgtgcaca tgttcaattt attaaagaaa agaaacggaa cactacacaa
     5041 agatataaag atcttgcttt gttggtgttt tttacttcaa ttgctttcag cttgttattc
     5101 gaaagcacga cacgctttat tcctttttta ttggttatct ggtttgtatt cttaaatttt
     5161 gtatataaaa gaataagcaa tagaaatatc agttaggccc agatacattt tgcaaaacga
     5221 attatttcag acccccctga aataatcgat acgcctgaaa aaacttccct gcgtataata
     5281 acagtatcaa ttaaacagga ggggatagga tgaactggta cagattgacg gtagaagatg
     5341 tattgaaaaa ctatcaaaca tcaacagaag gcttgtcgca agaagaacga cagaaacgcc
     5401 tgactgatca aggatataat gaaataaaag caaggcagca gacaaaacga tggaaaaaaa
     5461 tagcaaagca ttttactgat ctgctgatga tcgtgttgat cattgcctct tttttgaaat
     5521 ttgcttctag tgaatatatt gaaggaagta ttattttatt cgtagtgatc gtcaatggac
     5581 tcgttagtta ctggcaagaa agaaaagcgg aagaatcatt gaatggtttg aagcagctga
     5641 tgggacaaga agcgatagta ctatcaaacg ggatgcaaga aaaaatccct gcgaaagaac
     5701 tggtaaaagg agatgtcatt gtcttacgtg ccggagatgt cgttccagca gatatccgat
     5761 taatcgaagc acatgattta ttagtggaag aatcgatttt gacgggagaa tcagaagcat
     5821 ctgaaaaaag tcatgccgtt ttgacagaag aagaatcgat cggcgaccag aaaaatatgg
     5881 gtttttcagg aacgcttgta caatctggtt ctgcagtcgg tgtcgttgta gaaacaggaa
     5941 acacgacaga aatcggtaaa atcaatcaag cattgcaatc ggtcgaacaa caaacgacgc
     6001 ctctgattaa aaaaatcaac cgattaaaca aacaaatttt tcaaggcatt ctttgtttga
     6061 gcctattctt agttattttt acgacatttc gatatggtat ggattggcat atccttcttt
     6121 cggctacgat tgctttggtg gtgtcaatgg tacccgaagg attgccagct gttttgacca
     6181 tgattctatc cgttggagta catgaaatgg caaaagaaaa ggcgatcatc aaaggacttc
     6241 cttcggtaga aacattaggt tctatgacag tgatctgttc agataagaca ggaacattga
     6301 caaaaaatga aatgacagtt gtggatgtag ctgcaaaaga agaagcctgc gtgttgtcca
     6361 ttatgaaaaa ctgtcaggaa ttaaaaacaa aagaagaaca gaaaacagaa aatttaagtg
     6421 gcaatccgac agaagttgcg ctgattcgac acacagaaac agccaatcta cctttgaaaa
     6481 aaatagaagc aaagattccg ttcagttctg aatacaaata tatggcaacg atgcatgcgg
     6541 aggaaaaagg agcggtgatt tacgtcaaag gtgcaccaga agtattattt gctaaatcaa
     6601 ctctttcttc tgcagaacaa gaagcatgga gtcagacagc tgcagaattt gcgagaaaag
     6661 gtcagcgtgt tttaggattt gcttataaaa aagtagattc gaaacaggaa ctcacgcatg
     6721 agactttaac ccaattaact tttgcaggga tcgccggatt gatcgatccg ccaaaagaaa
     6781 gtgcagtcaa agctgtaaag gaatgccaac aagcagggat ctcagtgaaa atgattacag
     6841 gggatcacaa agacacagca aaagcgattg cggaacaaat tggcttaaaa catactgcca
     6901 aagttttaga agggatcgat ttggatctta tgtctgatga agagttgatt cagcaagtgc
     6961 caatagtcga tgtctttgct cgaacgacac cagaacataa actgcgaatt gttaaagctt
     7021 tgcagaaaaa tggagaaatc gtcggaatga ccggagacgg cgtaaatgat gcacctgctt
     7081 tgaaaagatc cgatgtagga attgctatgg gaatcaaagg tagtgaagta agcaaacagg
     7141 cagctgatat ggttttagga gatgataatt tccatacgat tgcaaaagcc gtaaaagagg
     7201 gccggcggat tttggataat ttgcaaaaaa ccatcaattt cttcttgcca actgcattag
     7261 ctcaaggatt gatcttgatt tgggcactta tgctgaatcg cccgctacct ttgtccccag
     7321 tacagatttt atgggtcaat atggtaacga caatcacttt atcttatgca ctagggttcg
     7381 agtcgccaga tccagaaatc atgaagcgcc caccaagaga tccaaagcaa ggaatcctat
     7441 ctggttatca tttgtttcgg atcatctatg tctccctact gatcatggtt cctgcctatt
     7501 tattggctat gcagtttgaa gggcaaagct tacagcaaac cattcttctg caaaatatcg
     7561 tcttagctca agctgtttac atgatcaatt gcagagagtt gtccaagccg tcattgaacc
     7621 aaggtctttt gcaaaacaaa gcgctttttc tttcattagg tatccttgct ttattgcaag
     7681 ggatggttat ttttctacca gtcggccagc agctgatcgg tacagtaagt ttaacgatga
     7741 cacaacagtt attgattgga gcaaatgtga ttcttctatt cttagttgta gaaatagaaa
     7801 aagggatcat gaacaaactg tttcgcaaaa aagaaaaaac agctagagaa tacagtcgct
     7861 agccgttact ataaaaaagt tttgaaagtg agttcagtca aacgattgag ctcatttttc
     7921 gttggaatag aagaaatgta tacaataggg atatctagag aatctagagg gaaattggaa
     7981 atcaagatat ctgttttttc aaaagaaata gtggaaagat cggttagttc gataggtaag
     8041 aagcagacac gttttcctaa gtaatctttt aattcctgct gcaagaacgc cttccatgaa
     8101 ggttctcctt ggaaaataaa atagacattg aacaaattcg gtttgataga aaaaatcgtt
     8161 tgttggatca gtagagacaa gagttggaga aagtaatttg gttcattgat ttctatttct
     8221 ttttttagat cgttcagtaa attctctttc tgttgataca agtgtgcata ttgctgatca
     8281 tatgtttcta aaaaacgatg gtattgacgg tccaatgctt caatcaatat cggcgattca
     8341 tgatatttta atatgaggag agccaagtta tcagtcagag atgttttttt tagttgatca
     8401 agagacagct ctgaagctag ttcctctaca aacgtttcga ggacctctaa aaatggagaa
     8461 tgatgcaggc agcgagcggt ttcttgtcca tagttattgt tgtaattcca actttccaga
     8521 aagcagaaag aagcaaaaga aagctcagtg tctttaagat aaataccttc cgttaaatac
     8581 aattttttca atgcagagtc atacagggag gagagtgtgc tgttggtatg gatagaagaa
     8641 tctaatcggc aattcgcttt tatacggatc gtattgataa agaaccaaca tgttccgaaa
     8701 atctcttctg tttcaaccgc tagttcagtt tgactgaggt ttcttaaaaa ttgaaaataa
     8761 tgggattcat ggatcgaata atcctcgaaa aaatagttgt tatgagtgaa aggaatcagc
     8821 aatcgatgat aaaaagtacg aatcgcacgc tcttttccaa gaatacaagc tgtatatttc
     8881 gtcaaatcga tctgcaagtc gtaacttgct aaacgctgat tgattttagt cacatgtctt
     8941 ttcaatgtcc cataagaaag cccatggtcg gctagaaaat cagggacagc aacttttttt
     9001 gtaaaaagca gatttttcag cagttcgaca ctgactgatt gtttgaaaaa aatctcccaa
     9061 aggtcatttg ttaataaatt ttgctggttt tgcaaactga ttcctgcttg atcgctgatg
     9121 atgtcccagc cttcgggcag ctgagagcga atagattgaa ggtcagaaaa aatcgttcgt
     9181 tcggttgtcc ctatgatttc agctaattca tttacggtta tctttgtatg attcagcaat
     9241 tgttctacta aagaaacatg acgtcgaata tctttttcgg taatgatttt tttcattagt
     9301 gaatacatca atcagactcc cttctattct aagaatacgg agaaaataaa cggtggtcaa
     9361 gtttcaactg atttttcaat cattgtttat ttttctggag catttactga tgataagaat
     9421 ttcatcatga acttgaaaaa tgataccact aaaatcaaaa aataggtggg caaacaataa
     9481 taaacatttt aatagtagat ggtagcaaga ccaacacagg cggattgaac atttaagagt
     9541 tgataaaaaa gaaaaatatc gaagtatttg attagcaact acttgtattc atttttctat
     9601 tcttacgtgg tatctttgaa taaagcgaat caataagaaa gagatggaag cagatgctaa
     9661 cgaaaaaaga actaatcgat cacatcaatg aaacatacaa tttgtctcca gaatatattt
     9721 ttaaaaaatt tcctgattat tgcgtattca gacatcaaaa aagcaaaaaa tggtttgggg
     9781 tcctgatgtc tgttccagaa gaaaaaatct atgatgatgc aagtgaccat aggatcatca
     9841 atattcttga tttgaaggtc gatcctgaac ttggcggaat tttgcgagga aaagaggggt
     9901 attttccagc ctatcacatg aataaagaac attggatctc tattgatcta cgtaaaatag
     9961 aaaagctaga tgaactagca gatttgattg atggaagttt taaatcaacg caatgaatgt
    10021 ctaggacaat agagaagagg ttgtgacaga aatgtttagc ttcaagaaat aagaaggaat
    10081 ttacgaaaat tgcttctcaa atttttggga aatttcggct tattttctaa gaaattactt
    10141 ctggaacacc gtttgttcga aaaaagagct gcgacaagaa ttttgtccca gctctttttt
    10201 ttttattttc ctaaataaaa ttcccgcatc gtttttgcaa tcattttgat caaaggatga
    10261 tcttctgtga agctagaaga taaataaatt gttcgtgtca agggcaaatc tttgatttcc
    10321 cgcaagacga aaggatcatt tttatcgtaa gaccaagaaa cttttgggaa aaagctaaca
    10381 gctaatcctt gcttgacaat agaacgtaat gtcgcaggat catctgtcgt cgaactgatc
    10441 gtcaattcga tacttttcgc ttcaagtgct gcatccagtg tatgacggaa ggcgttgctc
    10501 tttgacagca tcaagatggg ggtgcgttgg atatcttcta attgcagatg ttctttttta
    10561 gcaagaggat gtttcttagg aatggcgaca taaacaggtt ctttcatgat aggaatattg
    10621 gtcatgccag gcttcatctc cgaagtgatg tacagtacag gatcggtaat atcatctggt
    10681 aaatgatgtt gcatgatcgt taattggata tcaggatgct tttccataaa aattcggacc
    10741 aagttcggaa tagaaacaga ggctacctct aaatacaata tgacggaagt aacgatcttc
    10801 tcattgaccg aagacaataa tttgattcct tcttctaatg tgtctaatgt cttgactgca
    10861 tatggataaa attcttctgc ataaacggtc gtctcaatgt ttctgccgct atggtaaaac
    10921 aaagggacac ctaattcgtt ttccaaatgt ttgatcgcac ggctcaaggc aggttgagaa
    10981 ataaataatt ctttggctgc ttgggtaaca ttttttgttt cgaatacttt tttaaaatac
    11041 tgcagatgaa gcaatttcat aattatccat cccatccata cctttttgtt atggtttttt
    11101 ctttttttgg cattttttat tatagcagaa attgactaag atagcagtgg aaaaagtaag
    11161 tgctgaggag gcgaggctca tgagtacagg tttcttattt cctggacaag gcagtcagaa
    11221 gttagggatg ttagcggata tccctgaaat gtacatggaa cgtttagaaa atgtgacggg
    11281 gtatcggtta gttgataaag aagaaaacta tcaagatacg gtttttatcc agctagcatt
    11341 actgacaaaa gcagtatttt atttagatga aatggaaaaa aacggtattc agcctgatat
    11401 tgcagcagga cattcgattg gtgctttttg tgcagctgtt gcttgtcgat ctttgagatt
    11461 tgagaatgca gcggaactag tctatcaccg cgcagtctta atgaaagaag cttatccaag
    11521 cggttatgca atgggcgtag tagtaggtgt gacgagaagc gaagctgagg aaattgtagc
    11581 aaaaacattt gatcctgacc atccagttta tctgtcaaat gaaaattgtc cgatgcaaca
    11641 tacgttatct gggagtatcg aaggtttatc cgttacgttg gcagaagcca aacggcacca
    11701 agcgcaaaca gcgaagctgc tgaaagtccc tgtgccatct cactgtctct tgatgaatga
    11761 tacggtcaag aaattcaagc cgtatatgca gtctgtccat ctatctgatg ctagatgttt
    11821 gtatataaaa aatacggatg gaagagccac tcaggacgca gaagagattc gtaaagattt
    11881 attggaaaac ctagctttcc ctgtgcagtg gaaccaaatg atggacttgg caaaagaatt
    11941 aggaatggat ctgacgattg aatttccccc cggcaatact ttgacaaagc tgattcatac
    12001 aaaatttggt gtagataaag ggatccgtac gatcaatcta gaccaacatg gtattgatga
    12061 cgccacgttt ctatatcaaa aatggagatg actaattatg gaaaaaccaa accgcaactg
    12121 ggcttctaaa cgcgaaaaaa aagcgcagcg attggaaaaa atcagcaact tgctcaatgg
    12181 caagtatgta gaaactgaaa aaatcgtaga agtaatggaa aaactgattg ctcccggcga
    12241 caaagttgta ttagaaggcg acaatcaaaa acaagcaagt tttctttcag aaagtcttga
    12301 aaaagtagat gcagaaaaag tgaatgatct gcatttgatc atgtctagta tctcacgacc
    12361 agaacatttg aatatttttg aaaaaggaat cgcaagcaag atcgatttct cttatgctgg
    12421 tgcacaaagc ttgcgtgtcg cccaaatgat tgaagacggc acaatgaaat taggtgatat
    12481 ccatacttat ttggaacttt atggacgctt attcatcgat ttgattccaa atgttgtttt
    12541 agtagctgca gataaagcag ataaagacgg caacttgtac acaggtttca atacggaaga
    12601 aacaccaaca atcatcgaag ctgcagcttt caaagatggt atcgtgatcg ttcaagttaa
    12661 cgaagttgtc gattctttac cacgtgtaga tatccctggt gactgggtag acgttgtcgt
    12721 gaaagcagat aaaccttatc aattagaagc tttattcaca cgagatcctc aaaacatcac
    12781 agaattgcaa attctaatgg caatgatggc aatcaaagga atctacgcag aacacggcgt
    12841 acaatctttg aatcacggta ttggattcaa tacagcagct atcgaattgt tactgccaac
    12901 ttatggtgaa tcactagggt taaaagggaa aatcgcgaag aactgggtat tgaacccaca
    12961 tccaacgatg attccagcca tcgaatcagg ttgggttgaa agcatccata gttttggtgg
    13021 cgaagttggt atggaaaaat atattgctgc tcgcccagat gtcttttttg tcggtaaaga
    13081 cggcacgatg cgcagtaacc gaacattatc acaagctgca ggacaatatg ccatcgatat
    13141 gtttatcggt tcaaccttgc aattagatta cgaaggaaac tcttctaccg taacaaaagg
    13201 acgtttatct ggttttggcg gtgcgcctaa catgggacat aatcctggcg gtcgtcgtca
    13261 ttctacacca gcatggcaat cacttcgcga caatgatgat ccattaggga aaggccaaaa
    13321 attagttgtt caaatggttg aaacatatgg ttcaaataaa aaaccagtct ttgtcgaaga
    13381 attagatgca ctagctgttc aaaaacaagc aggcttagcc aatgcaccag tgatgattta
    13441 tagcgaagat actacacata tcgtgacaga agaaggaatt gcttatttgt acaaagcaaa
    13501 aacaaaagaa gaacgacaag cagcaattgc agcaattggc ggtgttacac ctcttggaat
    13561 gactagcacg aaagaatcac tagatagctt gcgtgcagcc ggcatcgtga aattaccaga
    13621 agatctaggc atcaaacgta gtgaagcgaa acgttcatta ttagctgcac aaaccatcga
    13681 tgatttgatc gaatggtcag atggcctgta tgaaccacca atgaaattca gaacttggtc
    13741 ataaaattgg cgagaaaaac cttttgtcgc catttaccca gatggaagaa gaaagacgaa
    13801 taagcagaac gtgtatgctc tgttttacaa aaaggggaga gatccatgga acaattagct
    13861 tataaatatc caacgaaaca gccgatcaat aagaaaatcc atgtaggagt ggtcggttca
    13921 ggagatttgg aaatcttgat gtttccaaca gaaaaaacag aatctactgt aaaaatttgt
    13981 acaggaagcg atggcttcgg cgaagtatgg aacaatgtat taacgagatt ttttgatcgt
    14041 tatccaattt cagcagatat cgtgatcaat gacttcggtg ctacaccagg agttgtgtac
    14101 ctacgattga cgcaagcaat ggaggaactt tcagatgaaa aatagttttg tagaattaaa
    14161 cgccagagaa cgagtgaacg ctctacttga caatggacgt gaattgattg atccattcga
    14221 tcaaatcatc gcgccaagtt taccagcaca aggaatcgtc cctgaaagtg atgacgggat
    14281 cgttgtcagc cgtggcacga tcaatggcaa acaagcagta gtaatcgcta tggaaggaaa
    14341 attccaaggt ggcggtatcg gtgaagtttc cggtgcaaaa atcgttgctg cacttgaaca
    14401 tgtgttagaa gaaaataaaa aaggaaatga gatctttcca gtcattgtac tagatacagg
    14461 tggtgttcgt ttgcaagaag caaactacgg actgttatct atttcagaaa tcggaaatgt
    14521 aatcattgct ctaaaacaat atgtaccagt catcggtttg attccaggac gtgtaggatc
    14581 atttggcggt atgtcgatta cttcagcatt aatgagttat ttaatcgcaa caaaaaaagc
    14641 gagagtcggt ttgaatggac cagaagttat tgaacaagaa gcaggcgttc gagaatttga
    14701 ttctagtgac aaagacttga tttggaatac attaggatct cgtcaacggg tagaatcaaa
    14761 aatcattgat gaattagtga ctgatagtgt agaagcaatc aaagctgctg tcatcgcagc
    14821 aatggacgaa cgaaaagaca cgcatcgcag tgaaagctca gcattttatt tatcattact
    14881 tgattcttta gatctttcaa aaccaatggc cattgacgat tacaatgaag caattgctaa
    14941 ccatcaagca aaatcaattg acttaccaga agtagcagaa ggaaatgaac cagctgcagc
    15001 aagtgttggt tacgattggt ttgcggcact aactggcatg aaaaatccaa cttcttctat
    15061 tagtactgtg tattatggta aatcaagtaa attcatagaa gaagccatcg tgacatcaat
    15121 cgtacctgat cctaaaaacc cgatgtaccg tgtacgaaac ggtgaagttg gtttagttga
    15181 aggtttccgc atgggacaaa ttttggatca cgtttacgaa gaagataaag acaaaaaagt
    15241 gaaacggcca atcatcgttg tgatcgatgt accaagccaa gcatacggct ataacgaaga
    15301 attgatcggt atccatgtgg ctttggctaa tagtgctgct gcttatgcaa aactccgtca
    15361 agctggacat ccagttattg gattgattgc aggaaatgct atttcaggtg catttttagc
    15421 acatggatta caatcaagcc gcttgattgc tttaaacaat aaagaaatta ccgtccaagc
    15481 gatgtctaaa gaaagtgctg cacgtgtgac aaaacgttcg gttgctgaaa tcgaagcagc
    15541 tgctgaaaaa gttccagcta tcgcttacga tatcaataat tatacaaaac taggagcgat
    15601 ctatcatttc ctagaagata tcaccgacca agctgcttca gaacaaaatg tcagcaaagt
    15661 catcgaagca atcaatgaag ccattcaaga tgtccgcaat acaaatgaca cgatgctgac
    15721 aaatcgatac acaaacgaaa cagccaaaac attcggacgt gtcgaaacaa ataaagtccg
    15781 tgccaaaatg gacgaagaat gggatgcata aaaaatgatt caagcacatg atattgtcct
    15841 ttttgataaa catgaaatag aagggttcac cttgcctgaa tgggtcgttc taactgaaaa
    15901 gatgttcgga acagttcgtc gtgcgtcgac ggatcacgca gaaatactac cgattggttt
    15961 acgtggacct gcccgcaatc agagatggtc aacagaaatc tcaaaacatg ccgtcaaaca
    16021 agtcattcat ccgtgggaca tcatcgaaca agaatcgtat agacaagaga aaatcatgga
    16081 ttatcctgtt tatcagcaat ttcatgctgc tagaaacata ttatcaggat gtaaatgggg
    16141 aatcggcggc agcttaggat ttgaattatc gactgggatt ccggcagtaa aagaaaccag
    16201 tgattttgat ttgctgctgt acgcaaattc accgattgaa ttgccaattc aggtaattca
    16261 atcgcatcct gctttttttg agcaattcga tacccaagta gtgactcggc aaggcggttt
    16321 ttcactaaaa gagtatttga gagagccaga gaaaaaaatt ctattgaaaa cagtcgaagg
    16381 acctaaatta accaaagaac tttggtaata aagaaaaaga gagagaaaga gatgtttgaa
    16441 aacaaaaaaa tcaaaatcaa cgtaagtgac ttccagaaag tatcagctgt aacggctgtt
    16501 atgatgcaaa cgatattgag ctttgctttg gcaaacagcc acagcgcaac aaatacagct
    16561 tttcttggta ccttttacgt catcgttaaa tacacagcac caatgtttat ttttgcaatt
    16621 gtttataata tggtaaaaac aagtcagcat ttgtcttatt gggaattttt gaaagaaaag
    16681 ttttttgaac tagtcgttcc gtatcttcta tggacgacgg cttatctttg gctatttcca
    16741 gcagtccaac aacaaactcc gtatacggat actgcaagct ttttactgaa atatgtcaca
    16801 ggtgatggag cgcctcattt atggtatacc gttatgatgc tgcagatcca attgttcatg
    16861 ccgttttttg tttggctcgg ttacaaagta tttgctaata aaaaatatgt atggccagta
    16921 ttgattggtt caacgatttt atatgtcgca tggtatctgt tctatcaaac acaagtattg
    16981 actgggcctt atcatgacag ctggtactta ttagatcggt ttgttgctag tttcatgatt
    17041 tatggggtag ccgcatttgt ttatcacgaa aaagtctatc aatatttgga cagagtacgt
    17101 tatcttttct tacctgttgc cttagttatc gcgttctttt ctgtccgttc attgctcgca
    17161 catccaggag acttgagctt tgcgaatgca ccttacttga atacgatcca aagtttatac
    17221 agtttagtca tcatctttgc tgtcttcata ggcgcttcca aaatgattgt aaacgattcg
    17281 ccaaaattgc cattgttcaa atggttgtct gtttatgctt atcgtacgta tttagccaat
    17341 gtatttgttt tccaagtact gttattattg tttaaagata gttggctgca gttaccttca
    17401 ggagtcatga ttcttgtagc ttatctaatg acggcaagtt gtgcatttgc tttaagctgg
    17461 ttacttcata tcatttgggt agctatcaaa aaaggtttca gcaagtaata gtaggaagaa
    17521 ggagtgagtg tcatgataaa tgacgaacga aaaaaagcag tgactcagca actcgctgcg
    17581 ttggtagagg aagcattgat cgcagaagta acattaagtc caaaacctgg attagtggat
    17641 gcactggatg cgggtgcaca ttccgatatg gactatgctt tatttcttaa gtctgctaag
    17701 acgctaactc ctttttttga agaaatggct caaatagcat ggcatcatgc tattgatcag
    17761 gagttaagag aaagaattgc tgagattggg cgaaaagcgg aaaaggcaat gctgattgct
    17821 acaaatggca tcaataccca taaaggagcg atttgggcaa tggggctatt gatcagtgtc
    17881 attgcacaaa aaatcagtat ggaacagcct ttttctttat cgaaaatact tgatgatgtc
    17941 agcaagcttg ctgcttttcc tgatacaaaa tatcgtcata aaaaaaccac tcatggtcat
    18001 agggtgaagc aaaaatatgg cgtaaatggt gcatatgaag aagcgatatt agggtatccg
    18061 catatcaggc ttgcaattca agcagctaag gagctagata atgattcata tgtacagaaa
    18121 caaatgcata tgttgttagt tttgatgagt tcgctagatg acacatgcgt attgcatcgc
    18181 agtgatcaga aaacgttagt ggaaatgcaa aaacttgctc atgctgctag tcaaagtgct
    18241 ttaccaaatc gtccatttac agcactgttg cacttttgtc aggaaaacca aatctctcct
    18301 ggaggaagcg cagatctctt gtctgcaagc ttgttcatgc tgaagataga ggaattatgg
    18361 actgccgaca catacatttg tccgatgtta ctcaatgact aaataagaca aaattgctaa
    18421 aaaaacagtt tatgcatctg gttattgact cttcctttta gcaaggctat cataaaaata
    18481 gttttttgta ataaaagata acgaggaaga caatgaaaaa aatcaattat ttagcaatca
    18541 ttttgttagt gggcctatgt ttgtttgctc tgccgaccca aggattcgct gtcgcgggtg
    18601 gtcatggtgg aggtacatct agcggtcgaa gttcgggcag aggttcattt ggtggtcgtt
    18661 catatggggg cagttatttt ggcagtcgtt cttacggcag agattcttat gggtattatg
    18721 gtccaggtac aggtccttct agttatgagc aaacgggatt tcttgcatta gctgttttgt
    18781 cagtaggagg ggtttctgct ttttctatcg tcaagaataa aagacaaaag aaaaggctga
    18841 aagcggagat attgtcccgc atgtcaggaa cggctaaaga aaagaaagca cagtttctgg
    18901 aaatccaaaa gatattcatg aagatccaat ccgcttggga agaagaaact cctgaaaaag
    18961 ccagaagctg ttatacagac aagctgtttg ccgaacatca gaaagttctg caaaaaaaca
    19021 aagcagacgg tgtaagaaat catactaaaa aaataactat tcaggaagta ggaaattatc
    19081 gacagatcca cgaaaatagt tttgccatcc gaattgattt cagatgctgt gattattcag
    19141 tcgatcgaaa agatgggcga ttgatctcag gaagtaagac acaacgacag tatttttctc
    19201 agatatggta ttttgatttt tctgagaaag aaaaaagatg gcaagccgat tttattcagc
    19261 cgattatgct ggattgacag gaaatattta tgaacggagt tgtgacaaaa gccttgtcgc
    19321 agctcctttt tctgaataaa cggtgtgcca gagatagcct atcggcaata aggcacgatt
    19381 ttgtaaaata tgggaagcga tttttcaaaa tcgcttctta tcgctcaagg ctgaacatct
    19441 ctgtcacaac ctctccacta aatggtgttt aaaagcttgc atctgcgata ataagaacaa
    19501 ccaaacgaaa aataggaagc actattttag tttagttgtt cttattactt gatgcaggac
    19561 agctttttca caacctcttt tttgtataga tgggcaaaat ttttgtaata ataggcgtat
    19621 gtctttttcg ttgaggagat gaaaaagcat atggcttatt ttttgtttgc tttgcttact
    19681 ttctttccac ttcaagtggc agaaatatct actttttatt atgtaagcgg aaccatatac
    19741 ttgaaatcag aaggagatca tttctatgaa aacaagaaca attgaaatac ttcacagaat
    19801 catgacagcc caatatccag taaaagcaaa acatctagca agtgagttca atgtcagtca
    19861 gcggacgatc cgtcaggaag ttcttgaagc gaatatctgg ttgagcagta aaaagcttcc
    19921 ggagatccgt acaattcgca acaaaggttt tttgttgaag ctggaaaagg aaaagcaagc
    19981 tcgattagaa actgaaataa aagatgtgaa agacggcttt ttgaatcggc gcgaacgtat
    20041 ttttgatcta gttttagccg ttgcttacag ccgaatcccg atccatctaa acaaaaaaga
    20101 agaagaattt caaatctcta aaagtacatt agatgaagat atgagacggt tacgagcaac
    20161 actggtcagt tatgggatag agatcgtcag ttatggaaag caaggactgg tttataaagg
    20221 ttcagaacga tctatccgta cgatgatcta tgattttatc aatcagaatc tgggaatgac
    20281 cgacttcttt atgaatcaag atgcacaatt aacaccggct caaaaaatct ttctccatta
    20341 ttttcctttg gtggaaatca aaaaaataca ggagatctat tccaataagt tgttgaaaca
    20401 agaagatgat atttataaaa aacaaatcct gctttttaca ttgatctgga tccagcgaat
    20461 caggaaacat gaatttatct ctgccattaa ttggaaaaat acagaaataa aagacagtag
    20521 cttttctgca tttattgacc acatgatcga ggaatttcaa ttggacaatg tcccgcaagt
    20581 agaaagaaat tatcttcgtt tcactattga gacgttcaat gctagagata tctccaattc
    20641 aatcgagtgg gtacaagctc aattactaac gatccaattg atccaatttg tagagaacga
    20701 gacacacgta cctttccatc taaaggaaga agctttattc gaaaatctct ataagcacat
    20761 ggctgcactg attgtaagaa tcaagaatca tatccaagtc atgaatccat tgaaagaaaa
    20821 catccgaaca aattatttcc caatctaccg cgcagtcact cattttgttc ctacgatcga
    20881 agaagtaatt ggtggcaaga tactggagga cgagatagct tttcttgtca tccacttttc
    20941 gacgatcgcc agtgtaatca agcgagactt gaattatgtt tataaatcag tcgttgtctg
    21001 taaccatggg atggctactg gaaatctgtt agcagagaat ctgaaagaaa agtttcctca
    21061 gatcgagatt actgcagttt taagttcaaa agaaatcgaa ttagtagata aattggatgt
    21121 caatctggtt ttcagcacct ttcaattgaa ttatcgaaat aagccgcttt tagttgtcga
    21181 tccgatttta actgagacga atatcttggt tatagaagac tttttgaaaa gaaatgaaga
    21241 actacagcgg ttagagccta acagtcatga atcgacggag ttattcactc aaataataga
    21301 tgtcatgaaa gaaagtggtg gccgagtaga tcgaatcatc tatggcaagt tagaaaaatt
    21361 attcgaaaca aacaatttag aaatcaacaa aagggagatc cagcctatgt taaaagatat
    21421 tttgacggac agcaatatca tgatcaaaga aaaagccact acttgggagg aatcgattga
    21481 actagctgct agaccattgg ttaaagaaaa cattgtcgaa gagcgctatg tcgaagcgat
    21541 gattcgtgca gtgaatgatt acggaccgta tatcgtgatt ggcaaacaca tggcactagc
    21601 ccatgcccga ccagaagatg gggtcaataa attaggagtc agcgtagcta cgattgagca
    21661 accaatcgat ttcggtaatc ctgaaatgga tccagtaaag attatttttt gtttggcagc
    21721 agtagactcc tactcgcatc tgaacatcat gaaagaacta attgaattga tcaacaacga
    21781 agagaagttg gatcgattga ttgaatgcac aaatatcgaa gatttcaaac aactgctttt
    21841 ttcagtagtt gagaaataat aaatgatacg ggggagaaca aagatgaaca aattgaacat
    21901 tttattcgta tgcggtgcag gtttaggaag tagttttgca gcacagatgt ctgctgagga
    21961 tgttttgaac aaattaggag tagaagccaa actggaccat tgtgatattt cttccgcagt
    22021 ttccatgaaa ccagatgtga tcatcacagc agaaaatttt cgttcgcagt tcgaaaagtt
    22081 cgcagttgat cctaaaacgg cgatgatcta tttgaaaaat attgtttcaa aaaatgaaat
    22141 tgaagaaaaa atcaccccag ttctgcaaga taagggagtt atctaaatac aaaaagaaaa
    22201 gagggtaaaa a
//
LOCUS       DAZKLM010000050        21941 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3764
            SAMEA115706913-rid22582563.denovo.051, whole genome shotgun
            sequence.
ACCESSION   DAZKLM010000050 DAZKLM010000000
VERSION     DAZKLM010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706913
            Sequence Read Archive: ERR13196647
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21941)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21941)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287823.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287823.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,004
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,849
            CDSs (with protein)               :: 2,849
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 41 of 96
            Pseudo Genes (incomplete)         :: 52 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 15 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21941
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706913-rid22582563.denovo.051"
                     /isolate="VRE3764"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(160..1227)
                     /locus_tag="ACG842_001658"
     CDS             complement(160..1227)
                     /locus_tag="ACG842_001658"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFQ0248203.1"
                     /translation="MQRVLMIGNDSSVKGGITSIITQMLNFNWEKKNIEMTFIPSYKG
                     GNAIFKSLYFMYAFAKIIGISLLSKPNIIHMHMSYRGSFSRKYVLNKWFRLLNIPVII
                     HLHGSEFKKWYDSVSVKKQNQIRLLLRESDSTIVLGEKWKKIIKEIEPNCKTVVVENS
                     IVVPEYTIKWNSKMCRFLFMGVLIKRKGVHDLVEAVKNIAEQYPEKKFIFEIAGAGDE
                     EISLKELIEEYNLQSYFEFTGWVVGEKKEECYRRSNVMILPSYNEGLPISILEAISYG
                     MPVIATDVGDISSAVRDGENGYLFQPGDVSALTSAILKINDKDKYVKFSRCSKDLAIT
                     KFNDGNFFDRILNLYQKGSKE"
     gene            complement(1247..1726)
                     /gene="pssE"
                     /locus_tag="ACG842_001659"
     CDS             complement(1247..1726)
                     /gene="pssE"
                     /locus_tag="ACG842_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PssE/Cps14G family polysaccharide biosynthesis
                     glycosyltransferase"
                     /protein_id="HFQ0248204.1"
                     /translation="MIFITLGSQKFQFDRLLKAIDDLIEKGVIIESTFAQSGYSDYKP
                     LNYSYKAFLNRDEFTETMQKAEMVITHGGTGAIIGAVKKGKRVIAVPRLAKYGEHVDD
                     HQLQLIRQFDEMNIIYPCYELENLEDAVKETRVRMFNKYVSNTETIINSIEVLIEKI"
     gene            complement(1743..2021)
                     /gene="pssD"
                     /locus_tag="ACG842_001660"
     CDS             complement(1743..2021)
                     /gene="pssD"
                     /locus_tag="ACG842_001660"
                     /inference="COORDINATES: protein motif:HMM:NF041549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PssD/Cps14F family polysaccharide biosynthesis
                     glycosyltransferase"
                     /protein_id="HFQ0248205.1"
                     /translation="MCTILINLFKSIIIYIKEKPDLVICTGVLAMIPICLITKMMGKK
                     LIYIESFAKVTSPTQTGKLMYHFADQFYVQWETMLQFYPNAIYLGGIY"
     gene            complement(1973..2203)
                     /locus_tag="ACG842_001661"
     CDS             complement(1973..2203)
                     /locus_tag="ACG842_001661"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0248206.1"
                     /translation="MNKKICFAASSGGHYEQLMMLKPLMEKYESFVITEQTEYSAESK
                     GEKTYYMRQVNRKEKFFCVLYLLIYSNQLLYI"
     gene            complement(2217..3467)
                     /locus_tag="ACG842_001662"
     CDS             complement(2217..3467)
                     /locus_tag="ACG842_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HFQ0248207.1"
                     /translation="MKKKYNIAVAGTGYVGLSIATLLAQHHQVTAVDIIPEKIELINK
                     RKSPIQDEYIEKYLTEKELDLTATLDARSAYKSADFVVIATPTNYDSRTQHFDTSAVE
                     SVIEVVMDVNPDTIMVIKSTIPVGYTESVRETTGSKNIIFSPEFLRESKALYDNLYPS
                     RIIVGTDMTDERLLNAAHVFATLLQKSAIKENVDTLFMGFTEAEAVKLFANTYLALRV
                     SYFNELDTYAEMKGLNTQQIINGVCLDPRIGTHYNNPSFGYGGYCLPKDTKQLLANYA
                     DVPGNLIEAIVESNRTRKDFIADRVLQMAGYYNYAEYNQYDVNRENPVLIGIYRLTMK
                     SNSDNFRQSSIQGVMKRVKAKGATVVIYEPALKDGETFFGSKVINDLEQFKAMCDSII
                     ANRYDTCLDDIKEKVYTRDIFQRD"
     gene            complement(3845..5296)
                     /locus_tag="ACG842_001663"
     CDS             complement(3845..5296)
                     /locus_tag="ACG842_001663"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HFQ0248208.1"
                     /translation="MYKKSSRGWVKHIDFIILDLLCIQLAFYLSYVLRQVDWNPYVIP
                     MYQNMAVFIELADLLVIFMFEAYRGVLKRGYYIEFASSVKQAIMLVLLGSLYLISVQG
                     GNDYSRVVLFLMGGLYVVITYITRLLWKHYLKHKMKNEGNKSLIIVTSSSIAEVVIRN
                     VKEHNYKMYKINGVIIIDKSMIGEDVEGVTVVSDSKNAADYLCQGWVDEVFIILNKST
                     SCMKEFIERCSEMGLTVHLNLAKVSDSALGKQSIGRIGEYTVLTTSINVMTTQQAFIK
                     RTIDILAGIVGCIATGIIFIFIAPLIYINSPGPIFFAQERIGQNGKPFKMYKFRSMYM
                     DAEERKVELMKENKMSNNLMFKMEFDPRIIGNKILPDGTKKTGIGQFIRSTSLDEFPQ
                     FFNVLTGKMSLIGFRPCLKSEYEEYDYHHRARIAIKPGITGMWQVSGRSDITDFEEVA
                     KLDTEYIKNWSMGLDFKILFKTVKTVLQRGGSM"
     gene            complement(5617..6381)
                     /locus_tag="ACG842_001664"
     CDS             complement(5617..6381)
                     /locus_tag="ACG842_001664"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CpsB/CapC family capsule biosynthesis tyrosine
                     phosphatase"
                     /protein_id="HFQ0248209.1"
                     /translation="MIDLHCHILPGIDDGAENLEASIAMAEKAIQQGITHILCTPHHN
                     NGKYSNEKSQVISLVASLQAELEKRQLPLTLLEGQEVRITGTLIEDIHRDEILFTDLD
                     DTYLLIEFPTLEVPLYAERLFFELLELGKTPVIVHPERNVHFREDPNHLLPFLDMGCL
                     AQLTAPSYVGVFGKDIQKTAKTMVKHNLVQMAASDAHGVSKRTFYLKECYEQIAKDFG
                     KEKVVQMKQVAKDLINGDEISYPVYEEVKKKKFGLF"
     gene            complement(6409..7107)
                     /locus_tag="ACG842_001665"
     CDS             complement(6409..7107)
                     /locus_tag="ACG842_001665"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004713 - protein tyrosine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0004715 - non-membrane spanning protein
                     tyrosine kinase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HFQ0248210.1"
                     /translation="MARTQKQKAAQTHAVSLITLTNPSSPIAEQYRTIRTNIQFASAA
                     GQQIKTIVVTSSGPGEGKSTTAANIAVVFAKSGQRVLLVDADLRKPVVYKTFQLNNAS
                     GLSTALSSSGSVADVIQRTPVENLSILPSGPKPPNPSELLSSPRMDQILSEARQLFDV
                     VIFDMPPVVAVTDAQIMSLKTDGTLLVVRENMSRKESLTKAKELLEMVQARVLGVVYN
                     GAEHSKDAGYYYGN"
     gene            complement(7119..7898)
                     /locus_tag="ACG842_001666"
     CDS             complement(7119..7898)
                     /locus_tag="ACG842_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzz/FepE/Etk N-terminal domain-containing
                     protein"
                     /protein_id="HFQ0248211.1"
                     /translation="MEETISLKEIFETLRKHLVIIIISMFAGLAISGIVTFFILTPKY
                     SSQAQLIVTLPQSDTANVNDVNTNLQMINTYKDMIVSDLVLNEVKDRLETEDNVKMTA
                     EQIKDAISVNQAENSQMFSIQAISTNAVAAQQIANTTSQVFQENAKDVMNVDKISIIS
                     GAVANTTPVSPNNKLNLMTGLMLGMMVGVGLAFLLELLDRTVKDEKYVTDTLGFPILG
                     TVPEMSAKELNAGIRKKGTAPARPKATNDKTAPSRRSRSRV"
     gene            complement(7914..8858)
                     /locus_tag="ACG842_001667"
     CDS             complement(7914..8858)
                     /locus_tag="ACG842_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HFQ0248212.1"
                     /translation="MARRRRKSRGKKIFLIILAVLIVLVLAIVGVGAKLYLDLSHSIE
                     KTYESVERTQGERTKPVDLDKQESFSVLLMGIDTGDLGRVDQGRSDTMMVATVSPEDK
                     QTTIVSIPRDTYVDIVGRDTKDKINHAYAFGGAAMAMDTVQKYVDIPIDHYVSINMKG
                     LKELVDAVGGIEVNNDLTFSQDGYDFPIGKITLNGDQALSYSRMRYEDPNGDYGRQGR
                     QRKIVEGVAKKVLSLNGVSNYQSVLNALESNMKTDMNFDEMKKIALDYREAFGTIKQD
                     QLQGEGFMQDNISYQKVSDQELDRVKKELKDQMNLENK"
     gene            complement(8904..9683)
                     /locus_tag="ACG842_001668"
     CDS             complement(8904..9683)
                     /locus_tag="ACG842_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0248213.1"
                     /translation="MEKPFVKLLATVAVGTGAIVICLFGYHLNNQRQYHQRINYAESA
                     ITNQKDTVTSLSKEVDKLYSTKEKIFLNPEITEETVSNLSHKLSSIKLSADDYDIKES
                     ELPKEAAAIQEEKKAVLTQLEDAESKLKIQTAVNKLFTKNVSNWQQAVDDVIIKEKLA
                     SADVAHVRENMSFFKDSAWKTVVMQYLGFADTQIAQVTQLDQLFDTMLKDGQVTATAT
                     YDQYLTALSQIEQIRNEKIRAAYATKAQTVAQQMGYSNISY"
     gene            complement(10063..12138)
                     /gene="glyS"
                     /locus_tag="ACG842_001669"
     CDS             complement(10063..12138)
                     /gene="glyS"
                     /locus_tag="ACG842_001669"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HFQ0248214.1"
                     /translation="MAKDLLLEIGLEEMPAHVVTPSMKQLEEKTAKFLDENQLTYDTI
                     ETFSTPRRLAIKVTNIPERQADSEEEVKGPAKKIALDADGNWTKAAEGFVRGQGLTTD
                     AITFRELKGVEYVYVTKYTHGKEAKEVLTGLAGVIKSLTFPVTMHWAEYDFEYIRPIH
                     WIVALLDDEVIPFEILDVKTGRASRGHRFLGEDVTFAHAKEYEEKLNEQFVIASPQKR
                     QEMIVTQANELAEKNHWVIDLDEDLLEEVNNLVEYPTVFSGDFEEKYLSVPEEVLVTS
                     MKEHQRYFEVRNDQGMLLPHFISARNGDRVKLEDVVRGNQKVLTARLEDAEFFYNEDK
                     KASIDQYVEKLKTVTFHEKIGSIYEKMQRVQVIARIIGEQVGLSEEELMDLQRAAEIY
                     KFDLVTNMVGEFPELQGIMGEKYALLKGEKPAVATAIREHYMPISSEGELPQSTVGSV
                     LAIADKLDSIFSFFAVGMIPSGSNDPYALRRQAYGVVRIIESKKWAFPLSVLQETISD
                     VISKDTDRFGIGLSEGQQQVIDFIKGRLRQLLTTKNIRHDVIDAVLNAEQKDLTKVFA
                     AAQLFKQHLADEDFKPSMEALTRVVNLAKKAQLEKQSEVDPELFENDAEKELHKAVEA
                     MKASFADQTIHENYDQLVALRPLIENYFDQTMVMADEPAVRKNRLTQLSQIAAMALSL
                     ASLDQIITK"
     gene            complement(12140..13057)
                     /gene="glyQ"
                     /locus_tag="ACG842_001670"
     CDS             complement(12140..13057)
                     /gene="glyQ"
                     /locus_tag="ACG842_001670"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HFQ0248215.1"
                     /translation="MSKKLTVQDMILTLQKFWSSNGCMLMQAYDTEKGAGTMSPYTFL
                     RAIGPEPWNAAYVEPSRRPADGRYGENPNRLYQHHQFQVVMKPSPENIQELYLESLRL
                     LGIDPLEHDIRFVEDNWENPSMGCAGVGWEVWLDGMEITQFTYFQQVGGLPCKPVTSE
                     ITYGLERLASYIQEVESVYDLEWADGVKYGEIFKQPEYEHSKYSFEVSDQDLLLSNFD
                     RFEKEAKRCIDENLVHPAYDYILKCSHTFNLLDARGAVSVTERAGYLSRIRNMARAVA
                     KIFVAEREKLGFPLLNKNTANPETVKEAE"
     gene            complement(13443..14261)
                     /gene="recO"
                     /locus_tag="ACG842_001671"
     CDS             complement(13443..14261)
                     /gene="recO"
                     /locus_tag="ACG842_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HFQ0248216.1"
                     /translation="MSQLAESKGLILSSRNYKEKDKLVKIFTESSGKMMFYIKEIHRQ
                     NQPLAPALLPFTEAVYIGNFRSEGLSFLSSVKDVHAFRHIQEDIFLSGYGTYLLNLVD
                     AAIEDHQYDPHLFQFTQQALQRMDQGDDAEIITNIFEVQILQRFGIAPIWTHCVVCGE
                     TKGKFDYSSKYGGVICERHWSMDDHRYHADPRAVYFVRMFSAISYDKISGIHLKEETK
                     QAIRELIDQLYDEYVGIHLKSKKFINQMKSWAHVLKTSEKDMSEKEKETPEKEE"
     gene            complement(14404..15303)
                     /gene="era"
                     /locus_tag="ACG842_001672"
     CDS             complement(14404..15303)
                     /gene="era"
                     /locus_tag="ACG842_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HFQ0248217.1"
                     /translation="MPEHKSGFVAIVGRPNVGKSTLLNRIVGQKIAIMSDKAQTTRNK
                     IQGVYTTPEAQLIFIDTPGIHKPKHRLGDFMVEAAYSALKEVDAVLFMISADQKRGRG
                     DDFIIERLKNVHSPVYLIINKIDKIHPDELLGIIEDYSTQMPFAQVIPISATEGNNVE
                     RLMDVLVSEMPEGPQYFPDDQVTDHPEYFIVSELIREKVLFLTRDEVPHSVAVVVDSM
                     KRNENDKIHIQATIIVERDSQKGIIIGKGGKMLKQVGTKARKDIENLLGDKVFLELWV
                     KVQKDWRDKKVYLQDFGYRKDEY"
     gene            complement(15318..15713)
                     /locus_tag="ACG842_001673"
     CDS             complement(15318..15713)
                     /locus_tag="ACG842_001673"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HFQ0248218.1"
                     /translation="MDLKDKNVEKNKHFLMSVEFAVQGVKTVFEEERNMRKHVAFGIL
                     ALFMGVIFQLDRIEWLWLLLAVFLVWIVEIINTVFENVVDMFTDFHFHPIGKKIKDMA
                     AGAVLVTACFTVIVGLILFVPKIYHLLFN"
     gene            complement(15697..16173)
                     /gene="ybeY"
                     /locus_tag="ACG842_001674"
     CDS             complement(15697..16173)
                     /gene="ybeY"
                     /locus_tag="ACG842_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HFQ0248219.1"
                     /translation="MDITFMDETNQVSEEKMKEIDDLLQFAASYLELPEETEMSVTFM
                     DNAAIQVINRDYRGKDAPTDVISFALEEEGEDEIPVIFEDGENPLPRVLGDIMISTER
                     AAEQAEAYGHSYDRELGFLAVHGFLHINGYDHMTPEDEKEMFGLQKEILDAYGLKR"
     gene            complement(16202..18394)
                     /locus_tag="ACG842_001675"
     CDS             complement(16202..18394)
                     /locus_tag="ACG842_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing metalloprotein"
                     /protein_id="HFQ0248220.1"
                     /translation="MLNKTLQKLQEKLGKAALPFLLFLFFIIVCGVTFSSVKQKAIDF
                     REGQVAEESIRANKTIENTEETEQRRKLAAEAVTPEYTYQKDLADDQNNRIKQLFELI
                     DKTNDTINKSYNGKVDKAKEDENIPKPTTEERIATLKKNFENVNAENVAFYQKLPNNF
                     YSTIFEMTETEIHTVRDESLKLIDEQMSKQVRESELEAFKQEAEDQIQYLNVTTEQQQ
                     MIRYLVDQGIVVNDVLNEKKTEELKQSAREAVQPVMIFQGEIIVREGNQIDASAMKKL
                     ELLGLTNQTTSIFPLIAMVLAVLLQIGVLIYNSLQFQNAGERTKYVLFYVTAMSVSVV
                     LMKFFQLFQTEQAAYIPLFYPAAFAPLVLSFFLNRRAGIMAGIFQAVSALFIFYDSIG
                     TNFLTVILMSYVFSGLMATIVRRKRISEQGLSAAMWVIIFPVMMDVILIIYQGMSFGD
                     ATTWLTLVCGFTGALFSFLLTIGLHPYIELMVNDDSVIVLNELSNPNHPLLKQLLEEA
                     PGTYHHSMMVANLSANAVAEIGGRSLLTRVACYYHDIGKIKHASFFVENLPSGAENPH
                     NFLLPEDSKHIIFGHVTDGAKILEDYNMPQMVIDICRQHHGTTLMRYFYVKAKERNPE
                     VTEEQFRYPGPKPQSREAGIVNIADSCEAAVRAMDHPTSEKIESFVHNLIEERISDGQ
                     LDDSGLTLKEIRKVEKSLISGLSSTFHSRIKYPKMKSEVEKIKEEQEE"
     gene            complement(18418..19389)
                     /locus_tag="ACG842_001676"
     CDS             complement(18418..19389)
                     /locus_tag="ACG842_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HFQ0248221.1"
                     /translation="MTEESSSLEIRLTAADDVSMLLGAHDKHIKLIEETTETTIHTRG
                     EIIQIIGEQSQISLAASVIRTLQELIKRGIHVSTPDVVTALKMGQKGTLDYFVEMYEE
                     EIVKDRNGKPIRVKNSGQKKYVESVAKHDIVFGVGPAGTGKTFLAVVLAIAALKKGEV
                     QKIILTRPAVEAGENLGFLPGDLKEKVDPYLRPVYDALYQIFGLDHTNRLMERGVIEI
                     APLAYMRGRTLDDAFVILDEAQNTTVAQMKMFLTRLGYQSKMIVNGDTSQIDLPRGTT
                     SGLVHAERTLKQIKKIDFVNFEASDVVRHPVVAEIIKAYEKADLHQE"
     gene            20094..21473
                     /gene="brnQ"
                     /locus_tag="ACG842_001677"
     CDS             20094..21473
                     /gene="brnQ"
                     /locus_tag="ACG842_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HFQ0248222.1"
                     /translation="MEETKTYSWKQLLMVSSLIFGMFFGSGNLIFPVHLGQMAGANWV
                     SAGVGFAISGALFPLLAILAVVVTKSDGLYDLAKPVGKWYAALFLILVHLTIGPFFGT
                     PRTAATAFEMAAKPFLPEKYNSLGMFLFSALFFLLAYFLTVHPTRLVKYVGKYLNSLF
                     LALLAIIFVVSLFHPMGNLNQSAADTYQTNALTNGVLEGYNTVDAVALLALSVTFVHA
                     VRDLGFKDKKVTELTAKAGSLSVALEVMIYFGLVLLGAMSLNKMSLSENGGTALSQIT
                     NFYLGNFGASFLGVMVTLGVFTTAMGLVVSFAQDFHKLFPKVSYMTWLRLTTFVSFVV
                     ANAGLDNIIQWSLPVLMLLYPLSLALILLSLTAKFFQKTPFVYQVTMLFAAVPAVLDM
                     LANSPALVSQQRVVASMLEFYHHHVPFAALGLGWMVPTLLGYAGSLLFYYAYRLSGYK
                     QEANELPEE"
     gene            21755..>21941
                     /locus_tag="ACG842_001678"
     CDS             21755..>21941
                     /locus_tag="ACG842_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HFQ0248223.1"
                     /translation="MSYSEFTKELLDILDLNLIFHEDAFRKERINDETCFVFDGTLTY
                     QPEECFHCHYQNKQTIIK"
BASE COUNT         6150 a         4622 c         3699 g         7470 t
ORIGIN      
        1 cttgtactct tgctctatta caagcactaa aacactacta ttcatttgaa ttcttacacg
       61 gataaaaatt tacatcgtct atttcaatat tttttctctc tattttattc acttaataag
      121 ttcattacat aatcgaaaca ttttttattt tccttgtcat cactccttgc tccctttttg
      181 atacagattt aaaattctat caaagaaatt tccatcgtta aatttcgtta ttgccaaatc
      241 cttcgaacaa cgtgaaaatt ttacgtattt atctttatca ttgattttta gtattgcaga
      301 tgtcaacgct gatacgtcac caggttgaaa caaatatcca ttttctccat cacgtactgc
      361 tgaagaaata tctcctacat ctgtagcaat tacaggcatt ccataagaaa tcgcctctag
      421 aatagatata ggcaatccct cgttatagga cggtaaaatc atcacatttg atcttctata
      481 gcactcctcc tttttttctc ccacaaccca acctgtaaat tcaaaatacg attgcaaatt
      541 atattcttct atcaattctt tgagcgatat ttcttcatca cctgctccag caatctcaaa
      601 aataaacttt ttttctggat attgttctgc aatgttttta actgcttcta ccaaatcgtg
      661 tacacctttt cgctttatca aaactcccat aaacagaaat cggcacattt ttgaattcca
      721 ctttatggta tattctggaa ctacaataga attttctaca acaacagttt tacaatttgg
      781 ctcaatttct ttaattatct tcttccattt ttctccaagt acaatcgtgg aatcagactc
      841 tcttaacagc aagcgaatct gattttgttt ttttacagaa acactatcat accatttttt
      901 aaattccgat ccatgaagat ggatgattac aggaatattt aataatctaa accacttatt
      961 cagcacatat ttcctactaa aactccctct gtacgacatg tgcatatgaa taatattagg
     1021 tttactcaat aaagagatac ctataatttt agcaaatgca tacataaaat ataaagattt
     1081 aaatattgca tttccaccct tataactcgg tataaaagtc atctcgatat tctttttttc
     1141 ccaattaaaa ttgagcattt gtgtaattat acttgtaatt ccacctttta ccgagctgtc
     1201 attaccaatc ataagtactc tttgcataac gctttactcc ttatatttat attttttcta
     1261 ttaatacctc tatcgaatta ataatcgttt ccgtgttaga tacatacttg ttaaacatcc
     1321 gaactcttgt ttctttaacg gcatcttcta aattttctaa ttcataacaa ggatagataa
     1381 tattcatctc atcaaattgt ctaattaact gcagttggtg atcatcgaca tgctcaccat
     1441 acttagccaa tcgaggaaca gcaatgaccc ttttaccttt ttttacggcc ccaattatag
     1501 cacctgtacc tccatgagta atcaccattt ctgctttttg catagtttca gtaaactcat
     1561 ccctatttag aaatgcttta taggaataat ttagtggttt ataatcacta taaccactct
     1621 gtgcaaatgt actttcgatt attactcctt tttcaattaa atcatcaata gcttttaaca
     1681 atctatcaaa ttgaaatttt tgtgaaccta atgttataaa tatcattttt ttctcctatt
     1741 atttaatata tcccacccaa ataaatagca ttaggataaa attgtagcat agtttcccac
     1801 tgaacataaa attgatctgc aaaatgatac atcaattttc ctgtctgagt agggctggtt
     1861 acttttgcaa acgattcaat ataaatcagt ttttttccca tcatttttgt tattaagcag
     1921 atagggatca tagctaaaac acctgtacaa atcacgagat ccggtttttc ctttatatat
     1981 ataataattg atttgaataa attaataagt atagtacaca aaaaaacttt tcttttctat
     2041 ttacttgtct catgtagtac gttttttcgc cttttgactc agctgaatat tctgtctgtt
     2101 ctgttataac aaaactctca tacttctcca tcagtggttt taacatcatt aactgttcat
     2161 aatgcccccc agaggaagcc gcaaaacata tctttttgtt catattaacc tctctatcag
     2221 tcacgctgaa aaatatctct agtgtaaact ttttctttaa tatcatccaa gcaggtatca
     2281 tatcgattgg caataataga atcacacatc gccttaaact gctctaaatc gttgataacc
     2341 ttacttccga aaaaagtctc tccatctttc agtgcaggct cataaatcac cacagtcgca
     2401 cctttagctt ttacgcgctt cataactccc tggattgaac tctgacgaaa attatcactg
     2461 ttgctcttca tggtcagccg gtatataccg attaaaactg gattttccct gttgacatca
     2521 tactgattat attcagcgta gttgtaatag cccgccatct gaagaacacg atctgcgatg
     2581 aaatccttac gggtacgatt agattcaacg atagcctcaa tcaaatttcc aggaacatct
     2641 gcatagtttg ccaacaactg cttggtatct tttggcaagc aataacctcc atatccaaag
     2701 gatggattgt tgtagtgagt tccaatacga ggatcgagac aaacaccatt gattatctgc
     2761 tgagtattca gccccttcat ttcagcataa gtgtctaatt cattgaaata gctgactcgc
     2821 agtgctaaat aggtatttgc aaacagcttt actgcttcgg cttcagtgaa tcccataaaa
     2881 agagtatcca cgttctcctt aattgcactt ttctgcagaa gtgttgcaaa aacatgagca
     2941 gcatttaaca agcgctcatc agtcatatca gtaccaacaa taatacgaga tggatacaga
     3001 ttatcatata aagccttgct ctcgcgcaga aactctggac taaaaataat gttcttactc
     3061 cccgttgtct cacggactga ttcagtgtag ccaacaggaa ttgtagactt gatgaccatg
     3121 atggtatcag gattcacgtc catcacaacc tcaataactg actcaacagc agaagtatcg
     3181 aaatgctgag tacgactgtc atagttagtc ggtgtagcaa taacaacgaa atctgcggat
     3241 ttataagcgc tccttgcatc caaagtagca gttaagtcaa gttctttttc tgtaagatac
     3301 ttttcaatat attcatcctg aattggagac ttccgtttat taatcagttc aatcttttca
     3361 gggataatgt ctaccgcggt tacctggtga tgctgcgcca atagtgtggc gatactcaaa
     3421 ccaacataac cggtgccggc tactgcgatg ttgtactttt tcttcacaat atctcctctt
     3481 tcctctctta agtatggaga atgttactcc tacggctatt catcatgtca aaataacaca
     3541 gcttagaatg ttgataaaat aattttccag aatgtgataa aatctctttc atttataaac
     3601 cacacatcta attttatcta ttctccacaa gacatgtaga gtattcttta ataacaagat
     3661 acgaccttac taaatacttc aaaaaattat catatttaat tcataaatat aaatcttcat
     3721 tttaaatgta tgcccaaaaa tttatctgta atgcgtcatc aaaatctaga ttccaaatct
     3781 ttatcaattt ttatccactc attctaccac tttagtaata ttttctattt ataacacttt
     3841 cgtcctacat tgatcccccc ctctgaagta cagtttttac tgtcttaaat aaaattttaa
     3901 aatctaaccc catactccaa ttttttatat attccgtgtc aagttttgct acttcttcaa
     3961 aatcagtaat atcacttcta ccacttacct gccacattcc agtaattcct ggcttaatag
     4021 caattcttgc acgatgatga taatcatatt cttcatactc tgatttaagg catggtcgaa
     4081 atcctataag gctcatcttt cctgttagaa cgttgaaaaa ttgagggaat tcatctaaag
     4141 acgtggatct aataaattga ccgattcctg tcttttttgt tccatctgga agtattttat
     4201 ttccgataat tcttggatca aactccatct taaacataag attattgctc atcttatttt
     4261 ctttcattaa ttctactttt ctttcttctg catccatata catacttcta aacttataca
     4321 tcttgaacgg tttaccattc tgacctatac gttcttgtgc aaagaaaatt gggcctggtg
     4381 aattaatata aataagtgga gcaataaaga taaaaataat tcccgttgca atacatccaa
     4441 caatacctgc aagaatatca attgttcgtt ttataaatgc ctgttgagtt gtcataacat
     4501 tgatacttgt tgtaagtact gtatattctc ctatacgacc aatagattgt tttcccaaag
     4561 cactatctga tacttttgca agattcaaat gtacagtaag gcccatttca gagcatcgtt
     4621 caataaattc tttcatacac gaagtacttt tattcaaaat aataaagact tcatctaccc
     4681 atccctgaca taagtaatcc gctgcatttt tggaatcaga aacaactgtc actccctcaa
     4741 catcttcacc aatcatgctt ttatctatga taatgacacc atttatttta tacattttat
     4801 agttatgctc tttcacatta cgaataacta cttcagcaat actactagat gtaactataa
     4861 taagtgattt gttcccctca tttttcattt tatgtttcaa gtaatgtttc cataagaggc
     4921 gagttatata tgtaattaca acgtataaac ctcccatcaa gaaaagaacg actctagaat
     4981 aatcatttcc accctgaaca ctaatgagat acaaacttcc taacagcacc aacataatcg
     5041 cttgctttac agaagaagca aattctatat agtaccctct ttttaataca cctctatatg
     5101 cttcaaacat gaaaataact aaaagatctg ccagttcaat gaagactgcc atgttttgat
     5161 acattggaat cacataagga ttccaatcta cctggcgtaa gacatacgat agataaaaag
     5221 caagctggat acacaataaa tctaaaataa taaaatcaat atgtttaacc catccccttg
     5281 aacttttctt gtacattcct ttttactccc ctctttttaa taaaataaat tgtattactt
     5341 gttttctctc ctaaatatct ttctgaagga ttattgactc gacaaattgg cttgtggata
     5401 ttcttatata tagaacatcc gtaagccaac ttaaaactcc ttaacaaaat ttttttactc
     5461 atattgtaga tcaaaacaag tatccaagaa atcatatttt atttcttttt tctgcttact
     5521 caacaacagc taaaagaaaa atcatcataa acaaattcaa ctaagccaac gatccccttg
     5581 tgaaatcaag ttttttcttg atttacgaat aaagagctaa aataaaccaa acttcttctt
     5641 ttttacctct tcatagactg gataactgat ttcatccccg ttgatcagat cctttgcgac
     5701 ttgtttcatc tgtactactt tttccttacc aaagtccttt gcaatctgct catagcactc
     5761 ttttaagtaa aaggtgcgct tagagacccc atgggcatca gaagcagcca tctgcacgag
     5821 attatgtttg accatcgttt tggctgtttt ctggatgtct ttgccaaata ccccaacata
     5881 acttggcgca gtgagttggg ccaaacatcc catatctagg aacggaagca aatgatttgg
     5941 gtcttcgcga aaatgcacgt tgcgttctgg atggacgatt actggtgtct ttcctagttc
     6001 taacaactca aaaaaaagcc gttctgcata taacggcact tccagtgtcg gaaattcgat
     6061 cagaagatat gtatcatcta aatctgtaaa taatatttca tctcggtgga tgtcttcgat
     6121 gagcgttccg gtaatacgta cttcttgtcc ttccaataac gtcaaaggca actgtctttt
     6181 ctctaattcg gcttgtaacg aagcgactag agaaatgact tgtgattttt cattgctgta
     6241 ttttccatta ttgtggtgtg gtgtgcataa aatatgcgtg atgccttgct gaatcgcttt
     6301 ttcagccatt gcaatacttg cttctaagtt ttctgccccg tcatcgattc ctggtaggat
     6361 atggcaatgt agatcgatca tttctcttgt ctcctctctt cttattcctt aatttccgta
     6421 gtaataacca gcatctttgc tatgttctgc tccgttgtac acaacaccta agactcgtgc
     6481 ttgtaccatc tccagcagtt cttttgcttt agtcaaggat tctttacgtg acatattttc
     6541 acgtacgacc aataaagtgc catccgtttt taaagacatg atctgtgcat ctgttacggc
     6601 tactactggt ggcatatcga atatcaccac gtcaaataac tgtcttgctt cggataggat
     6661 ctgatccatt cttggcgagc ttaacaattc tgatgggttg ggtggcttag gtccactagg
     6721 caaaatcgac aagttctcga ctggcgttcg ttggatcaca tctgctacac ttcctgatga
     6781 actaagtgct gtacttaatc cactggcatt gttcagctgg aatgtcttat agacgacggg
     6841 tttacgtaaa tctgcatcga ctagtagaac tcgttgacct gatttagcaa aaacgactgc
     6901 aatgtttgct gcagtggtgg atttcccttc tccaggacct gatgaagtca cgacaatcgt
     6961 tttgatttgc tgaccagctg cggaggcaaa ctggatattc gtccggatcg tacgatattg
     7021 ttcggcgatt ggtgaagatg ggtttgttaa tgtgatcaaa ctgactgcat gagtttgtgc
     7081 tgctttttgt ttctgtgttc gtgccatttt tcttcctcct agactcttga gcggcttctg
     7141 cgtgatgggg ctgttttatc gtttgtagct tttggtcgtg caggagctgt tcctttttta
     7201 cggataccag cgttcaattc tttcgctgac atttctggaa ctgttcctaa gattgggaat
     7261 cctaatgtat ctgtcacata tttttcatct ttcactgtcc gatcgagtaa ttccaataag
     7321 aatgccagac ctacacctac catcattcct aacattaagc cagtcattaa attcaattta
     7381 ttatttggcg aaacaggtgt cgtattggca actgcacctg aaatgatcga gattttatca
     7441 acattcatca catcttttgc gttctcttgg aaaacttgtg aagttgtgtt agcgatctgt
     7501 tgagcagcca ctgcattcgt acttattgcc tgaatcgaga acatttgtga attttctgcc
     7561 tgattaactg agatggcatc tttgatttgt tcagcagtca ttttcacatt gtcttctgtt
     7621 tctaaacgat ctttgacttc attgagcact aaatcactga cgatcatatc tttatacgta
     7681 ttgatcattt gcaggttcgt attgacatca ttgacatttg cagtgtctga ttgtggcagt
     7741 gtcacgatca attgtgcttg tgaactgtat tttggtgtca agatgaaaaa tgttacgatt
     7801 cctgagatgg ccaagccagc aaacattgag atgatgatta ttaccaagtg tttgcgtaat
     7861 gtttcaaaaa tttctttcaa gctgattgtc tcttccattt tttctccact ttcctattta
     7921 ttctctagat tcatttgatc ttttaattct tttttcacac gatcaagttc ttgatcactg
     7981 actttttgat aggagatatt atcttgcatg aagccttctc cttgcagctg atcttgctta
     8041 atcgtaccaa atgcctcccg ataatctaaa gcaatttttt tcatttcatc aaagttcatg
     8101 tctgttttca tattcgactc tagagcattc aaaacggatt gatagttgct gacaccgttt
     8161 agactcaata cttttttcgc tacgccttct acgatctttc tttggcgccc ttgtctaccg
     8221 taatcaccat ttgggtcttc ataacgcata cgagaataag ataatgcctg atccccgttc
     8281 aatgtaattt taccgattgg aaagtcatag ccatcttgtg agaaagtgag gtcattattg
     8341 acttcgatac caccaactgc atcaacgagt tcctttagtc ctttcatatt gatagagaca
     8401 tagtgatcga ttgggatgtc tacatatttt tgtacggtat ccatcgccat ggcagctccg
     8461 ccgaatgcgt aagcgtggtt gatcttgtct tttgtgtcac gacctacgat gtcgacatac
     8521 gtatctcgag gaatgctgac gatcgttgtc tgtttgtctt caggactgac tgtggcaacc
     8581 atcatcgtat ctgatcttcc ttgatcgacc cgacctaaat ctccagtatc aatccccatc
     8641 aatagaacag agaaagattc ttgtttgtcc agatcaactg gtttcgttcg ttctccttgg
     8701 gttcgttcca ctgattcgta agttttctca atagaatgtg agagatccag atataatttt
     8761 gctcctactc ctacaattgc taatactaat acgataagaa ctgctaaaat aatcaaaaat
     8821 atttttttcc ctcttgattt tcttcttcgt cttgccatag tttcctccgt ttgctaacct
     8881 tgcttgaatc aacgttttaa ctgtcaatag cttatattgc tatagcccat ttgctgagca
     8941 acagtttgtg cttttgttgc gtatgcggct cttatttttt cattccggat ctgctctatt
     9001 tgactgagcg ccgtcaaata ttgatcgtaa gtagcggtag ctgttacttg accgtctttc
     9061 agcatcgtat cgaatagctg atctagttgc gtgacttgtg caatttgggt atcagcgaat
     9121 cctagatatt gcataacgac tgttttccaa gcactatcct taaaaaagct catattttca
     9181 cgtacatggg cgacatctgc acttgccaac ttttctttga tgatgacatc atccacagct
     9241 tgctgccagt ttgatacgtt tttagtaaat aatttattga cagccgtctg gatcttcagt
     9301 tttgattctg catcctctaa ctgcgttaag actgctttct tttcttcttg gatcgcagca
     9361 gcttctttag gtaattctga ttccttgatg tcataatcat ctgccgaaag tttgatagaa
     9421 gatagtttat gtgacaaatt tgaaactgtt tcttctgtta tttcaggatt taggaaaatt
     9481 ttttcttttg tactgtacag cttatctact tcttttgata aagaggtcac ggtgtctttt
     9541 tgattggtga tggcagattc tgcgtaattg atccgttgat gatattgccg ctgattattg
     9601 agatggtatc caaacaagca aatcacaata gcgcctgtgc ctaccgccac agtagccaat
     9661 aacttgacga acggcttttc catagattca ctcctaaata ttgatgatag ataagtaaag
     9721 atgtgattca ttcacatcag aatgagcgct ttttatttta aaagcataat taaactaata
     9781 tattcataga aatatttata aacaggtttt ccctatataa tcataccaca tacagttttt
     9841 ttgactaggg aaattcctaa tttaaaagta aataaaacag taacaagatt ataattaatc
     9901 gttttcattt ttatttcctc acttttcatg agcgttttga aaattcgaac aaagaataaa
     9961 ataacaaaaa ttattaaaat agaattaata acagctattt ataacaaaaa aaacagagac
    10021 acagcacgtt tcgtgcatgc tctctgtttt tcaaacaaaa gactattttg tgatgatttg
    10081 atccaagctt gctaatgaca aagccatggc agctatttga cttagttgtg ttagtcggtt
    10141 cttacgtact gctggttcat cagccatgac catcgtttga tcaaaataat tttcgatcag
    10201 tggacgaaga gcgacaagct ggtcgtagtt ttcgtggatc gtttggtcag caaaacttgc
    10261 tttcattgct tctactgctt tgtgtaattc tttctctgcg tcgttttcga acagctcagg
    10321 gtctacttct gattgttttt caagctgcgc ttttttcgct aagttcacga cacgtgtcaa
    10381 agcttccatt gaaggtttga agtcttcatc tgcaagatgt tgtttgaaca attgtgctgc
    10441 tgcaaatact tttgtcagat ctttttgttc cgcattcaaa acagcatcga tgacatcatg
    10501 acgaatattc ttcgttgtca ataattggcg cagacggcct ttgataaagt cgatcacttg
    10561 ttgttgccct tctgataaac caatgccgaa acgatccgta tctttactga taacatcgct
    10621 aatcgtttct tgcagaacag acaaaggaaa ggcccatttt ttcgattcga tgatccgaac
    10681 gacaccgtat gcttggcgac gtaaggcata aggatcgttt gacccgcttg gaatcatccc
    10741 aactgcaaag aaagaaaaga tgctatctaa tttatctgca atcgctaaaa cagaacctac
    10801 tgtagactga ggtagctctc cttcactgga tataggcatg taatgctcac gaatcgctgt
    10861 cgctacagca ggtttctcgc ctttcagaag agcatatttt tcgcccatga tcccttgtag
    10921 ttcaggaaat tcccctacca tgttggttac tagatcaaat ttatagattt cagcagctcg
    10981 ttgcaaatcc atcaattctt cctcagatag tcctacttgt tcgccaatga tacgggcaat
    11041 gacttgtacc cgttgcattt tctcatagat actaccgatt ttttcatgga aagtaacagt
    11101 ttttagtttt tcaacgtatt ggtcgattga agctttcttg tcttcattat aaaagaattc
    11161 cgcatcttct aaacgagccg tcaatacttt ttgattgcct cgaaccacat cttccaattt
    11221 tacccgatca ccattacgag cagaaataaa atgaggaagc agcatacctt gatcattccg
    11281 tacctcgaaa taacgttgat gttctttcat agaagtgact agtacttctt caggaacaga
    11341 aagatatttt tcttcgaagt cgccagaaaa gacagttgga tattcgacta aattgttgac
    11401 ttcttctagt aaatcttcgt ctagatcaat aacccagtga tttttttctg ctaattcgtt
    11461 tgcttgtgta acgatcattt cttgtctttt ttgcggagac gcaatgacga attgttcatt
    11521 caatttttct tcatattctt ttgcatgcgc aaatgtcaca tcttcgccta agaaacgatg
    11581 acctcgagag gctctgcctg ttttgacatc caagatttcg aatgggatta cttcgtcatc
    11641 taacaatgcc acgatccagt gaatagggcg gatatattca aaatcgtatt ctgcccaatg
    11701 catcgttaca gggaaagtca aacttttgat cacccctgct aagcctgtca atacttcttt
    11761 tgcttcctta ccatgagtgt atttcgttac atagacatat tccacacctt ttaattctct
    11821 aaaagtgata gcgtctgtcg tcagaccttg tccgcggaca aatccttccg ctgctttcgt
    11881 ccagtttcca tctgcgtcca aagcaatttt tttggctggt cctttgactt cttcttcact
    11941 gtccgcttgt cgttctggaa tatttgttac tttgattgct aaacggcgcg gtgtggaaaa
    12001 tgtttcgatc gtatcataag tcaattggtt ttcatctaaa aattttgctg ttttttcttc
    12061 taattgtttc atgcttggtg tgacaacatg ggcaggcatt tcttccagcc cgatttcaag
    12121 taataagtct tttgccatct tattcagcct ccttcacggt ttctggattt gctgtgtttt
    12181 tattcaacaa agggaatcct aatttttcgc gttctgccac aaagattttg gctacagcac
    12241 gagccatgtt gcggatgcga gaaagataac cggcacgctc tgttacggat actgcaccgc
    12301 gggcgtctaa taagttgaat gtatggctac atttcaagat ataatcatac gccggatgaa
    12361 caagattttc atcgatacag cgtttagctt ctttttcaaa acgatcaaag ttgcttagta
    12421 gcaagtcttg gtcgcttact tcaaaagaat atttggaatg ttcatattct ggttgtttga
    12481 agatctcgcc atatttcacg ccatcagccc attctaaatc atagacactt tcaacttctt
    12541 ggatataaga agcaagacgt tctaatccat aagtgatctc agaagttact ggtttacaag
    12601 gcagtccacc aacttgctgg aagtacgtga attgtgtgat ttccattccg tcaagccata
    12661 cttcccaacc cacaccggca cagcccatgg atgggttctc ccagttgtct tcaacaaaac
    12721 ggatgtcgtg ttctaatgga tcgattccta aaaggcgcaa gctttctaaa taaagctctt
    12781 gaatattctc aggtgaaggt ttcatcacca cttggaattg atgatgctga tacaaacggt
    12841 ttgggttttc accataacgt ccatcagctg gtcggcgaga tggctctacg taagcagcat
    12901 tccatggttc tggtccgatt gcccgtaaaa aggtatatgg gctcatcgta cctgctcctt
    12961 tttctgtgtc ataagcttgc atcagcatgc agccgtttga tgaccaaaat ttttgtaatg
    13021 ttaaaatcat atcttggacg gtcaattttt tactcatctg ttttgcccct ttcaatttta
    13081 aagcgttgct gtcaaataag aatacaggaa agaaaacgac aaaaaatccc tatgtctgat
    13141 tacagacata gggacgaata attcgcggtt ccaccctact tccgaataaa gattcggcag
    13201 cttcatttat cgtactcgca agtgcctctt caaaaatcgt ttgggtccgg ctcccactct
    13261 cccgaactcg ctaaaccacg tgttttttac tgatcttggt caatgtacgt attcattttg
    13321 cagttttctt gtttctataa tagatcctat cactgaacga tctatttaat ctatggttaa
    13381 taatagacgg aactccctgt gtttgtcaaa tggttttgat tttttgttgt atgttttatt
    13441 tactattctt ctttttcagg tgtctctttt tctttttcag acatgtcttt ctctgacgtc
    13501 tttaatacgt gtgcccaaga cttcatttga ttgatgaact ttttgctttt caaatggata
    13561 ccgacatatt catcatacag ctggtcgatg agttcacgga ttgcttgttt ggtttcttct
    13621 ttcagatgga ttccactaat tttatcataa gaaatagctg aaaacatccg aacgaaataa
    13681 acagctcgag gatctgcatg ataacggtga tcgtccatcg accaatgtct ttcgcatatc
    13741 acaccaccat atttggaaga atagtcaaat tttccttttg tttccccgca tacgacgcag
    13801 tgtgtccaga ttggtgcaat tccgaagcgt tgcagtattt ggacttcaaa aatattcgtg
    13861 atgatttctg catcgtctcc ttggtccatc cgttgcagtg cttgttgcgt aaactggaac
    13921 aaatgcgggt cgtactgatg atcttcgatt gccgcatcta ctagattcaa taaatatgtg
    13981 ccatagccgc tcaaaaagat gtcttcttgg atgtgtcgaa acgcatggac atctttgaca
    14041 ctgcttaaaa atgacaaacc ttcacttcga aaattcccga tataaactgc ttcagtaaaa
    14101 gggagcaaag ccggagctaa gggctgattt tgccgatgga tctctttgat ataaaacatc
    14161 attttgccag aagattctgt aaagattttg actaatttat ctttttcttt gtaattacgg
    14221 ctagataaaa tcaagccttt tgattctgca agctggctca tattctccct cctaaaaaat
    14281 aaatctggaa aagcagattc tgccgatcca gatcatttat gttgcgatct tccgcagctt
    14341 ttctcaacac gcggattttt attcagttta tttctatacc taatttacaa gaagctcaga
    14401 atatcagtat tcgtcttttc ggtagccgaa gtcttgcaga tagacttttt tgtctcgcca
    14461 gtctttttga actttgaccc aaagttctag aaagactttg tctcctaaaa gattttcgat
    14521 atcttttctt gctttcgttc ctacttgctt cagcattttt ccgcctttgc cgataatgat
    14581 ccctttttgg ctatctcttt cgacaataat cgtagcctgg atatggattt tatcattctc
    14641 gtttcgcttc atcgaatcaa caacgactgc gacagaatga ggcacttcgt ctcgtgttaa
    14701 aaacaagact ttctcacgga tcaattcaga aacgatgaag tattcaggat gatcagtcac
    14761 ttgatcatct ggaaaatatt gtgggccctc tggcatttca ctgactaata catccatcaa
    14821 acgttcgacg ttgtttcctt ctgtagcaga gattggaatc acttgtgcaa aaggcatctg
    14881 cgttgaataa tcttcgatga ttcccaacaa ctcgtctgga tggattttgt cgattttatt
    14941 gataatcaga taaacaggag aatgcacgtt ttttagacgt tcaataataa aatcgtctcc
    15001 ccgaccgcgt ttttgatcag cactaatcat aaataagacc gcgtccactt ctttcaacgc
    15061 actgtaagct gcttctacca taaaatctcc taaacgatgc tttggtttat ggattcctgg
    15121 tgtatcaata aaaatcaact gagcttctgg tgtagtgtat actccttgga ttttatttct
    15181 agttgtctgt gccttgtcac tcatgatggc aattttttga cccacgattc gattcaataa
    15241 tgtggatttc cctacatttg ggcggccgac gattgcgaca aaccctgatt tatgttctgg
    15301 catatatgtt ctccttttta attaaacaac aaatgatata ttttagggac aaataaaatc
    15361 aacccaacga tgacggtaaa acaagcggtc actaaaactg ctccagctgc catatctttg
    15421 atttttttcc caatcggatg aaaatgaaaa tccgtaaaca tgtccacgac attttcaaaa
    15481 acagtattga tgatctctac tatccatacg agaaaaacag ctaaaagcag ccataaccat
    15541 tctatccgat caagttggaa aatcacgccc ataaacaaag cgaggattcc gaacgcaaca
    15601 tgtttcctca tgttgcgttc ttcctcaaaa acagttttga ctccttggac ggcaaattcc
    15661 acggacatca ggaaatgttt gtttttctct acgtttttat cttttaagtc cataggcatc
    15721 taagatctct ttctgtaaac caaacatttc tttctcatcc tctggtgtca tatggtcata
    15781 cccgttgata tgcaagaatc catgaaccgc taaaaagcca agttcccgat catacgaatg
    15841 gccgtaagct tctgcttgct ctgcagcacg ttcagtagag atcatgatgt ctcccagtac
    15901 acgaggcaat ggattctctc catcttcaaa aatcactggg atttcgtctt cgccctcttc
    15961 ttccaaggca aaactgatga cgtctgtcgg tgcatctttt ccacggtaat cacgattgat
    16021 tacttggatt gctgcattgt ccatgaaagt aacagacatt tctgtttctt ctggcaactc
    16081 taaatatgat gctgcaaatt gcagcaagtc gtcgatttct ttcattttct cttctgagac
    16141 ttggtttgtt tcatccatga aggtaatatc cattatacat cgcttctttc tattcttctt
    16201 gctattcttc ttgttcttct tttatcttct caacttccga tttcatttta ggatatttga
    16261 tccgagaatg gaaggtactg cttaaaccgc tgatcaatga tttttctact ttacggattt
    16321 cttttaacgt caatccactg tcgtccaatt gtccatcgga aatacgctct tcaataagat
    16381 tatggacaaa actttcgatt ttttcgctcg ttggatggtc cattgcccgt actgccgctt
    16441 cacaactgtc agctatattg acgattcctg cttcacggct ttgaggtttt ggtccaggat
    16501 aacggaattg ctcttccgta acttccgggt tgcgttcctt tgccttaacg tagaaataac
    16561 gcatcagcgt ggttccatga tgctgtctac aaatatcgat caccatttgc ggcatattat
    16621 aatcttccaa tattttggca ccatctgtca catgtccaaa gataatatgt ttactgtctt
    16681 ctggcaataa gaaattgtgc ggattttccg ctcctgaagg caagttctca acgaaaaagc
    16741 tggcgtgttt gatcttgccg atatcatgat aatagcaagc tactcgagtt agtaaagaac
    16801 gtccaccgat ttctgctaca gcattagcac ttagattggc taccatcatg ctatggtgat
    16861 acgtacctgg tgcttcttcc agcaactgtt tcaataaagg atgattggga ttgctgagtt
    16921 catttaatac gatcacacta tcatcattga ccatcaattc aatatagggg tgcagaccta
    16981 tcgtcagcaa gaatgaaaac agtgcacctg tgaatccgca gactagcgtt aaccaagtcg
    17041 ttgcatcccc aaagctcatt ccttggtaga taatcaaaat gacatccatc atcacaggaa
    17101 aaataatgac ccacatagca gcggataacc cttgttctga gatccgtttg cgtctgacga
    17161 tcgttgccat gagaccagaa aatacatagg acatcaaaat aactgtcaga aagttcgtgc
    17221 caatcgaatc ataaaaaatg aaaagtgcgg atactgcttg aaaaatacca gccatgatcc
    17281 ctgctcttcg attcaagaaa aagcttaaaa caagtggagc aaaagccgcg gggtaaaaca
    17341 gcggaatata agcagcttgt tctgtttgga agagttggaa aaatttcatt agtacgacgc
    17401 ttactgacat tgccgtcacg tagaaaagta cgtattttgt tctttctcct gcattttgga
    17461 attgaagtga attataaatc agtacaccga tttgaagcaa aacagctaaa accatggcaa
    17521 tgagtgggaa aatagaagtc gtttgattcg tcaatcctag taattccaat tttttcatcg
    17581 ctgaagcatc aatttgattt ccttcgcgaa caataatttc tccttggaag atcatcactg
    17641 gttgtacagc ttctcgtgca gattgtttta attcttctgt ctttttctca ttcaatacat
    17701 cattaactac gatgccttga tccactaagt agcgaatcat ttgttgctgc tctgttgtga
    17761 cattcaaata ttgaatctga tcttctgctt cttgtttaaa agcttccaat tcgctttcac
    17821 gaacttgttt actcatctgt tcgtcaatga gcttcaaact ttcatcccta actgtgtgaa
    17881 tttccgtttc agtcatttca aatatcgtcg aataaaaatt gttcggcagt ttttggtaaa
    17941 acgcaacgtt ttctgcattc acgttttcaa aatttttctt cagtgtagca atccgctctt
    18001 ctgtagtagg tttcgggata ttttcatctt ccttcgcctt atctaccttg ccattataag
    18061 atttgttgat ggtatcattc gtcttatcga tcaattcgaa taattgcttg atccgattgt
    18121 tctggtcatc tgccagatct ttttgataag tatattctgg agtcactgct tctgctgcca
    18181 gttttcttct ttgttcggtt tcttccgtgt tctcaatcgt tttattcgca cggatactct
    18241 cttctgctac ttggccttct ctgaaatcaa tggccttttg tttgacacta ctaaatgtaa
    18301 cgccgcagac aatgataaaa aacaagaaca gaaggaaagg caatgctgcc tttccgagtt
    18361 tttcttgtag tttctgcagt gttttattca acatgtgctt atcctcctaa gcaaagctta
    18421 ttcttgatga agatccgcct tttcataagc tttgatgatt tcggcaacta cagggtgccg
    18481 aacaacgtca cttgcttcaa aattaacaaa atcgattttt ttgatttgct ttaatgttcg
    18541 ttctgcatga acaagaccac ttgtcgtccc acgaggcaag tctatctgac ttgtatcccc
    18601 attgacaatc atttttgatt gataaccgag acgggtcaag aacattttca tctgagcgac
    18661 tgtcgtgttt tgtgcttcgt ccaagataac gaatgcatca tccaatgtcc gcccccgcat
    18721 gtaagctaaa ggtgcgattt cgatcacgcc tcgttccatc aatcggttcg tatgatccaa
    18781 tccaaaaatt tggtataaag catcataaac aggccgcaga taaggatcca ctttttcttt
    18841 cagatccccg ggtagaaagc ctaggttttc ccctgcctcc acagcgggcc gcgtcaaaat
    18901 gattttttgc acttcgcctt tttttagggc agcaattgct aatactacgg caagaaacgt
    18961 tttccctgtt ccggctggcc ctacaccaaa tacgatgtca tgtttggcta ccgattcaac
    19021 gtatttcttt tgaccgctgt tttttacccg tataggtttt ccattacggt ctttgacgat
    19081 ctcttcctca tacatctcaa caaaataatc taatgtacct ttttgcccca ttttcaatgc
    19141 cgtcacaaca tctggcgtac ttacgtggat tcctcgtttg atcaattcct gtaatgtccg
    19201 aatgactgag gcagccaagg aaatctggga ttgctcacca atgatctgaa tgatttctcc
    19261 acgtgtatgg atggttgttt ccgttgtttc ctcgatcagt tttatatgct tgtcgtgtgc
    19321 acctaaaagc atgctgacat catcagcagc tgtcaatcta atctctaaag aactagactc
    19381 ttccgtcaaa aatagacatc cccttttttt cgattcattc tttcctattt taccatactc
    19441 agaagaaact atatagcaaa taagaaaagg ttgtaaaaga gtcgcttaac catacgtacc
    19501 aagaaacgtt aaaaaatagc cgtttccatt cgctaaaggt ctcgttttga taaaaagaag
    19561 ctgagaatgt aagttatatg aaagtggaga acaaaatatt tcgtttcatt caacaagtat
    19621 tctctaggta tttttcctgt ttcaaaaaaa gaaaacaaaa tagttaaaat attcttgcat
    19681 ttcaaaaaaa acaggtgtat tattacgtca acaaataaac atacagcaaa gcgtcgagga
    19741 aaacagtagg ctattcaacg atttattccc agtgagtcat gtaagtgaga aatgacaata
    19801 aatcgaatag acgaacatca tttccgagtc aatgtcgaaa acgtagatca ttgtggctgc
    19861 ctccattatc aggctagcgg ataactttat caaaaagcgt ccgtgaatga agaagctttt
    19921 ttaagcgaaa aaaggtggta ccgttctaga atctagagcc cttttcccaa gtggaaaagg
    19981 gctttttttt attttaaggt ggtggttttc atggaatctg atattagtgg cattactaat
    20041 tgctgcaacg tttatatcgt caatcaaaat gataattatt ggaggaaaaa tttatggaag
    20101 aaacaaaaac ttattcatgg aaacaactat tgatggtcag ttcacttatc ttcgggatgt
    20161 tcttcgggtc aggtaatcta atctttcctg tccacttagg tcaaatggct ggcgcaaact
    20221 gggtcagtgc tggcgtagga tttgctatct caggtgctct tttcccatta ctagctatct
    20281 tagctgtagt agtgacaaaa agtgatggtc tgtatgactt agctaaacct gtcggaaaat
    20341 ggtatgctgc cttgttctta atccttgttc atttgacgat cggaccattt ttcggtacac
    20401 cacggacagc tgctactgct tttgaaatgg ctgctaaacc atttttgcct gaaaaatata
    20461 attcattagg tatgttttta ttctctgctc ttttcttttt acttgcttat ttccttactg
    20521 tccatccaac acggttggtc aaatacgtcg gaaagtatct aaactccttg tttcttgcat
    20581 tattagcaat catttttgtc gtttctttat tccatccaat gggaaatcta aatcaatctg
    20641 ctgcagatac ttatcaaaca aacgctttaa caaatggtgt cctagaaggt tataacacgg
    20701 ttgatgcagt agctttgctt gccttaagtg tcacttttgt ccatgcagtc cgtgaccttg
    20761 gtttcaaaga taaaaaagtc acagaattaa cggcaaaagc tggtagcctg agcgttgcgc
    20821 tagaagtcat gatctatttt ggacttgttc ttctcggtgc gatgagtttg aataagatgt
    20881 ctttatcaga aaatggcgga actgcacttt ctcagatcac taatttttat ttaggtaact
    20941 tcggcgctag cttcttagga gtgatggtca cattaggtgt cttcactacc gctatgggac
    21001 tcgttgtttc ttttgcgcaa gatttccata aactgttccc taaagtcagt tatatgacat
    21061 ggctgcggtt aacgactttc gtctcttttg tcgttgcaaa tgctggttta gacaatatca
    21121 tccaatggtc tttaccagta ttgatgcttt tgtatccatt gtcgcttgcg ttgatcttgc
    21181 tttccttaac agcaaaattt ttccaaaaga caccatttgt ctatcaagta acgatgttgt
    21241 ttgctgctgt tcctgccgta ttggatatgc tggcaaattc gcctgcttta gtgagtcaac
    21301 aaagagttgt agcaagcatg ctcgaatttt atcatcatca cgttcctttt gctgcattag
    21361 gacttggctg gatggtccca acgttacttg gttacgctgg cagcctactg ttttattacg
    21421 cctatcgcct atcagggtat aaacaagaag caaatgaact accagaagaa taaccaaatc
    21481 aaacacaatt taacataaga aatattaaac aggaaaaaac cagctagatg aaagaatcct
    21541 attctttcgt ctagctattt tttagttctc ttttttacgc gcacaaaaaa actgcggaca
    21601 atgattgtcc gcagtttaaa tgggctctat aatatctagt gttggtgacg ccttctgttg
    21661 ccaatacttt ttcttttttc taataaacgc aaacagacac cttgaaatcc tttagaataa
    21721 agtcgacgaa aaccattcaa aggagtgttt caagatgtcc tattcagagt ttaccaaaga
    21781 gttactagat attctagacc taaatcttat ctttcatgaa gatgcctttc gcaaagagcg
    21841 aatcaatgac gaaacatgtt ttgtttttga tggcacattg acctatcaac cagaggaatg
    21901 cttccattgt cactatcaaa ataagcaaac gatcattaaa t
//
LOCUS       DAZKLM010000051        21234 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3764
            SAMEA115706913-rid22582563.denovo.052, whole genome shotgun
            sequence.
ACCESSION   DAZKLM010000051 DAZKLM010000000
VERSION     DAZKLM010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706913
            Sequence Read Archive: ERR13196647
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21234)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21234)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287823.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287823.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,004
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,849
            CDSs (with protein)               :: 2,849
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 41 of 96
            Pseudo Genes (incomplete)         :: 52 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 15 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21234
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706913-rid22582563.denovo.052"
                     /isolate="VRE3764"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(36..749)
                     /locus_tag="ACG842_001679"
     CDS             complement(36..749)
                     /locus_tag="ACG842_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016611336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFQ0248224.1"
                     /translation="MKKIAKYMEIYLDIKKKIVNGEYKIGEKLPTGDELAVIYHTSKL
                     TVKKGVDLLVSEGVLRSRSGYGTEVVRTPIDNSRVLGPNEGLFSVVGEEHVDSEIHTF
                     SIELPSKKVADKLSISVKDYVYNIVRSRFIDCQPYSIEQTFMPLAVIPGLEPKHLKKS
                     VYAYITNELGLEIKTSHIRIKGDTANEFDAKILKIDPGEFMIEIDKVVALSSGKPFEY
                     SVSRHLYQDFVFEAVFVEK"
     gene            924..2348
                     /locus_tag="ACG842_001680"
     CDS             924..2348
                     /locus_tag="ACG842_001680"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFQ0248225.1"
                     /translation="MTTMKKLTFPDDFWWGSAWSAEQAEGKGETGKAETIWDRWFKEQ
                     PYRFYQRIDSSITTDHIHQYKEDIELMKKTGHNSFRVSISWARMFPNGGTGKVNPKAI
                     SFYRKLFSEMNNNGIKVFANLYHFDMPATLQDKGGWESREVVDAYVHYAETCFKEFGD
                     LVCHWFTFNEPLGPILGTYLEDFHYPNLIDFKRGAQAAFFTILAHAKAIQVFKELALP
                     SKIGVILNLSPTFPRSQNPADVKAAEIADLFYTRSFLDPMVKGNFSKKLILLLQEYNQ
                     LPEDYTKEDLVCIYENTAQILGLNYYEPRRVKSRSSMVDTSSPFLPEWFFEPHIMPGR
                     RMNEYRGWEIYEKGIYDLCMDIKNHYGNIEAFISENGMGVADEERFMDDTGQIIDDYR
                     IEYIKDHLAYLWKAINEGCNIKGYHLWTFIDCWSWINAYKNRYGLVSLDLTTQKRTIK
                     KSGEFYKQLSDTNGFRYDVDLLVE"
     gene            2647..3354
                     /locus_tag="ACG842_001681"
     CDS             2647..3354
                     /locus_tag="ACG842_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HFQ0248226.1"
                     /translation="MDEKLDIKTAIKDTLPTVFGYIGIGLAFGIVGKAAGFHPLVVTL
                     MSLLIYAGSAQFITVSMLASHSPLLSIVLSTFLVNSRMILMSMTIAPYFKKNRLLQNL
                     LIGTLLTDESFALGMNKLNYTGQKLNFRWMNTANWISYLTWVGSSLVGALLGNFITDP
                     KKFGLDFAIVAMFIGLLYLQVISDRNTSKRLQLILIGLTLILVYVGLIFIPSNLVILV
                     VTLIGCGLGVWIKHAFF"
     gene            3341..3664
                     /locus_tag="ACG842_001682"
     CDS             3341..3664
                     /locus_tag="ACG842_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HFQ0248227.1"
                     /translation="MPSFEFTLLTILGCTLATWLSRILPFVLLKKFDLPQPLLEYLSF
                     VPIVIMSALWFSSLFTQNIGHLPQINLENALASVPTLLAAILSKSLLVIVLVGILSLS
                     LIRLL"
     gene            complement(3753..4790)
                     /locus_tag="ACG842_001683"
     CDS             complement(3753..4790)
                     /locus_tag="ACG842_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HFQ0248228.1"
                     /translation="MSEKELMKVVGFYEGLSIDDPKSFIDEKEQIPVPSSRDLLVKVN
                     AVSVNPVDTKLRQDNGIRNALRILGFDGVGKVVAVGDEVQKFSVGDRVFYAGTTTRAG
                     SNQEYQLVDERIVALAPKNLSDEEAAALPLTSLTAYELLFEKFGLTPEENANRGKKIL
                     VINGAGGVGSILNQLAHWAGLEVYATASPKNFEWLKKTGVDYPIDYHHDLSKSFRELG
                     IDQVEYVAVLFDITRYFDQIKHLVRPFGHVGTIVGIKEPLDIGVWKNQSISFDWEYMF
                     AKTDYNYEIETQGEALAHIAALANEGIIRSTLSKVYSDGMNAKNLKQATKDVETGHIL
                     GKVVVSGPFNG"
     gene            complement(5111..6094)
                     /locus_tag="ACG842_001684"
     CDS             complement(5111..6094)
                     /locus_tag="ACG842_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase"
                     /protein_id="HFQ0248229.1"
                     /translation="MTTNFWKELPKPFFVLAPMEDVTDVVFRHVVKHAAAPDVFFTEF
                     TNSDSFCHPDGKDSVRGRLTFTEDEQPMVAHIWGDKPEFFREMSIAMAEMGFQGIDIN
                     MGCPVPNVAERGKGSGLILRPEVAAQLIEAAKAGGLPVSVKTRIGYTEMSEMEEWISH
                     LLHQDIANLSVHLRTRKEMSKVDAHWEVIPKIIALRDQIAPQTLITINGDIPDRQTGE
                     ELAEKYGVDGIMIGRGIFKNPYAFEKEPREHTEKELIGLLRLQLDLQDHYSEIIPRSI
                     TGLHRFFKIYIKGFPGANDLRVQLMNTKSTDEVREILDTFEKEHPTLFSEQ"
     gene            complement(6509..7279)
                     /locus_tag="ACG842_001685"
     CDS             complement(6509..7279)
                     /locus_tag="ACG842_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0248230.1"
                     /translation="MFNLYFTALKSLAIKETNRYLRIWVQTLVPPVITTSLYFVIFGK
                     MIGGRIGDMGGFSYMEFIVPGLIMMSAITSSYANVSSSFFSQKFQKNIEEILVAPVPT
                     HVIIWGFVIGGVGRSILVGSLVTIISLFFVPLHVYSWFMVIITLLMTAILFSLAGLLN
                     GIFAQSYDDVSIVPTFVLQPLTYLGGVFYAISMLPPFWQAVSKVNPIVYMISGFRYGF
                     LGTIDVPVATSMIVLVLFIVVLYAVTWYLINKGRGLRS"
     gene            complement(7272..8207)
                     /locus_tag="ACG842_001686"
     CDS             complement(7272..8207)
                     /locus_tag="ACG842_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0248231.1"
                     /translation="MANALEINNLKKVYGSGVEALRGIDLSVKEGDFYALLGPNGAGK
                     STTIGIITSLVNKTSGKVNVFGYDLDTDLVRAKQQIGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVSRKEAMKRSEKYLKQSNLWEKRNVRARMLSGGMKRRLMIARALMHEPRLLIL
                     DEPTAGVDIELRREMWEFLRELNENGTTIILTTHYLEEAEMLCRNIGIIQSGELIENT
                     SMKELLSKLQFETFIFDLAPYETKPVIEGYACTFEDEQTIAVEVERNQGVNGIFDQLT
                     RQGIKVLSMRNKSNRLEELFLKITEENNHTEEKNV"
     gene            complement(8523..10172)
                     /locus_tag="ACG842_001687"
     CDS             complement(8523..10172)
                     /locus_tag="ACG842_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HFQ0248232.1"
                     /translation="MGQREFIVLIIIAAVILLVALDIFSRLKLKETVRSKWEKIPYQP
                     RFDKEESLKEAWLTEKKFRSWDSEIDDLTWYDLDMFEVFEGINSTYSSVGSEALYQRL
                     RSFDFGEDQQLEKLIAFYQENPQLREKIQYQFARLGKKDHNFAKQYLADGKSKKIGRV
                     GLFIFLGLLPLVGLGLLLLGQVLGIYLAIGGLVFNTVYYLVKKQTLETELNSMSYLVS
                     TISAANQLAKLPTPLQEELKQQTKPLRDIPKLGFSFRMKSGSEAELIFEYVNIMFMLP
                     FISYHFVLTKLEKHSTEAIKLWELLGELEVAAAILNYRTYMPITCRPEFTEGGVTAKD
                     IYHPLLKEAVVNPVNWQKNTLVTGSNASGKSTYVKSIAISCILAQTIQTAIAEQFTLQ
                     PGHVLTSMAIEDDLFEGDSYFVSEIKSIKRLLDQVASKERCYCFVDEILKGTNTIERI
                     ASSASVVRWLAEYPSLAFVATHDIELTEILKNYCENVHFEEQVTEGKGISFDFKLKEG
                     PSRTRNAIALLKVLDYPETLVETAKTEAALFDEKRQWHVLG"
     gene            complement(10289..12892)
                     /locus_tag="ACG842_001688"
     CDS             complement(10289..12892)
                     /locus_tag="ACG842_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HFQ0248233.1"
                     /translation="MVNAHIIQHTHWDREWYFTNEDAIVLSDQVFTEVLDELERNPKV
                     NFCLDGQTSIVDEYLEINPQKLKVIQRLMKTGQLFVGPWYAQTDALLVDAESILRNLM
                     IGISDTILKYGDPMMVGYLPDTFGFNANLPTLLHQVEIDNFMCWRGLNFETMVPSPYF
                     IWKGLGDKFVYAMNFPFGYMTGMMTLDAIKDIPEFAKEKLDEAVEMLHMQGKNKDILV
                     PSGVDQKSMILNFDQVVEKLNKYSKFHNIISDYPSYVEVIRNKKELPEYKGELRQPVY
                     SRVHRSIGSVRTKMKIENFKLEQKILKRVEPLMLIAERNGIHISKGLLRRLWKKILQN
                     QAHDSIGGCVSDNVAEDIFHRIKEANEIADGIENLIVKRMADALKLKSNEVLVFNTTT
                     ENYSGSKIVHVVADSKKIHFKGSPYNIIEKEVYFPERKNAYRMIATGFEYFDEPAYYE
                     LDIKINVNVPALGYTVVEFEKNNEKLETAIEIDKTEISNNKYKLSFKDGQLNLIVGDR
                     QYLDFVHLIDSANDGDTYDYSPLEGDTELSLKLETAKVYKDSLQETLVVYGKAQLPKD
                     LKDRLSEKPEMEEISYEISFSLGESQIVEGTLKIHNTVYSHRMRLAINVLDKSEKSIA
                     QIQNGFIENTPDVIPPNWSETMVEKPVNLEIFDKSVSVAENDHYFTFYADGLKEYERI
                     ENQLIITLFSTTGELGKPNLAWRPGRASGDTTNEGHVMMETPLAQEIGEYKVTFGFNV
                     EEGRFNEFKVAKQAEKRLEQSISYQKQKLNVFIHRLDNKIWPLQNELDVPRKFSLIEL
                     PEDLIVSSIYPSYFHEQGFVLRLANPTEQEKIVPEAILSLGTVVNALENKQELTTIPP
                     YDYLSILINER"
     gene            complement(12904..13953)
                     /locus_tag="ACG842_001689"
     CDS             complement(12904..13953)
                     /locus_tag="ACG842_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HFQ0248234.1"
                     /translation="MEFKKQGREILNHFQSGVSYMIPLVVAAGLLTSIAVIFGGTGVW
                     DQTDTFWGVLRMIGQTGLQFIVPMISAYIAYSIADRPGLAPAFITGMMCQNLGMGFIG
                     GMVAGLACGYLANALKKVKVPMQVISLKSIMLIPFVTTLVVGIILWYVLATPIQAMTT
                     GLTNWLEGMSGANAALMSAILGGMMAFDMGGPINKIAYAFGVASFSSGSFGPSTAMLL
                     GIAIPPFGMFLATILNKKLYDESERDNAKTAMIMGLVGITEGTIPFAVKDPLRVIPSI
                     VVGTAVACGLNGFFGVTQETMLSTFMAIPFVTNIPLYLVSIVIGGVVTALMVNALKSI
                     KYKKEQQVNSVEEGK"
     gene            complement(13979..14293)
                     /locus_tag="ACG842_001690"
     CDS             complement(13979..14293)
                     /locus_tag="ACG842_001690"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_function: GO:0090563 - protein-phosphocysteine-sugar
                     phosphotransferase activity [Evidence IEA];
                     GO_function: GO:0090582 -
                     protein-phosphocysteine-D-fructose-phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HFQ0248235.1"
                     /translation="MTKIVAVTACIAGIAHTYMAKANLEKFAKKKGLDIKVETQGAMG
                     IENKLSISDVSEADVVIFAVDTNVSERGRFDGKPILTVGTSEVVKDGEETIDEAIALI
                     NK"
     gene            complement(14309..14755)
                     /locus_tag="ACG842_001691"
     CDS             complement(14309..14755)
                     /locus_tag="ACG842_001691"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose PTS transporter subunit IIA"
                     /protein_id="HFQ0248236.1"
                     /translation="MISEKININNIVIDLNASDKKSVVEKLSKLLETNGFVSDHKQFQ
                     KDVFDRENHMTTGIGKNIAIPHGKSLAVIESTVAVAKLKRSVEWGALDDQPVDLVFLL
                     AIKDTDKGTNHLRILAELSGRLMDDEFVKRIKDSRNKKELVKALSF"
     gene            complement(14756..16678)
                     /locus_tag="ACG842_001692"
     CDS             complement(14756..16678)
                     /locus_tag="ACG842_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFQ0248237.1"
                     /translation="MGIEFSERQMKILYFLIENEHTTYKELSEQLYVSTKTISKDILI
                     LKEIIQKMPDVQMLVQPKKGVSLIGKKETLRNLLSSHKINIADSPEDRLTYVFAKLLN
                     TNDSLKIQNLADELYVSRSTVESTMKDVRKRLNKHGMSLVSDRNGVRIKADEKSKRRL
                     MSEVVNYYWGGFTASNSKTDLLDLNINFIGEANNFLNKDILKKVAELLNRYMNKTQLK
                     FTDYEFQTLAIHLAIAIERIETSNFLSRSNNSEEEISKNTLILIKMIEKAFSIIVPID
                     EQAYVDIHITSIEKNAMNHDLDQTKLSYLDMGVELQELVRIKLNEFYPDEELINNLVL
                     HLNAAVKRLKLGVNIYNPYTDKIKYSFKRSFMISVDLLEEIEERFCIHFNEDEIAYVT
                     LHIQSLLDRYKPDKTKVILVCSSGYGTSKLLEQRINNGFADMVEIEDVLSINELQDRK
                     VTDELVISTIPIENTNFPVIVVSPLMLGADVRKVKQYLQLNEQTEDAFLNLLDKRFCF
                     FTHQIKDNQVNVLNIITKKLVKENYAKEGILEGAIKREKLSSTAMNDFAMPHIEVDFI
                     KKPVICVYVNPEGIDWDGTLVHSVFFFALNEKVKPELNKIYETFNEIASNHELLANLK
                     TSSSFEKLVYILKEGL"
     gene            complement(16890..17786)
                     /locus_tag="ACG842_001693"
     CDS             complement(16890..17786)
                     /locus_tag="ACG842_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFQ0248238.1"
                     /translation="MIKWLLLLVVIFMGSFIFFLQREIKRLTKKIATLSERQEYGGRL
                     SVEFREKNLMDLVDALNTWLDENEQKGQAFQEMEENIRLSIVGLSHDLRTPLTAINGY
                     VQLLEQVEDTEKREKYLTIIKQSVARLLSMTDQFYDLARIETKQKEMDLHTLSFSKEV
                     EEVFFSFYEQFSEKNIEVAFAEHSINTTVLADPLMLLRVLQNIIQNLLRYADGQAKVT
                     FSKEDPFIRLTVANPIKPGTQTSVEKVFQRFYTENTSRTNTESSGIGLYLSKKLVEGM
                     GGEMTARLDGGIFSISVKLRRV"
     gene            complement(17779..18459)
                     /locus_tag="ACG842_001694"
     CDS             complement(17779..18459)
                     /locus_tag="ACG842_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFQ0248239.1"
                     /translation="MVLLETILIIEDDEAVHSLLEEVLEQRYKLLDAYSGTEGKLLLS
                     TYPVDLILLDLMLPGLSGEALLAEIRQTSNVPIIVLSAKSDQRDKVSLLAAGADDYVT
                     KPFDIEELLLRIGIQLRHQTSVQVKDLSQRIYYKEILLDKESRDVNVNGVAVHLTGRE
                     FDLLKLFLEHPKKVFSRANLYETVWQEPYFDSEKTVNMHISNLRGKLAKAHAVYIHTV
                     WGVGFRFD"
     gene            complement(18460..19242)
                     /locus_tag="ACG842_001695"
     CDS             complement(18460..19242)
                     /locus_tag="ACG842_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFQ0248240.1"
                     /translation="MFNLLTAEKIKVWKSRKMWVSLGLMVVLPLFYSWNEWYMHTKYG
                     NELNQATDTVINGATGILMVEKMAAWILLAFAAFACFYIGEEFQNGTIRNTLSLGRNR
                     MTYYVSKLLVTLLITTLGVVLITGLAMLAYTLAFGFGEVEGIKNYGNYVLKVFPVLLL
                     LTLGTLSVPVALTFITRSTSVSLLLSFLYIMGTAFIPGVFAKIKGLEFLTEWFTETWL
                     MYTDFAQQATYSQAPKMILVSLVTIVVSSALGMFIFQKSDIK"
     gene            complement(19235..20152)
                     /locus_tag="ACG842_001696"
     CDS             complement(19235..20152)
                     /locus_tag="ACG842_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFQ0248241.1"
                     /translation="MDFGNETVLKATGITKKYGAAKALDKVSIEIKRGMIYGLIGENG
                     AGKSTFMRTIMGLISIDEGSIELFGTTDLQAARRRMGQSIETPALYPELTARDNLRIQ
                     AANGGVSDREIEDLLKMMRLENTGKKKAKNFSLGMRQRLAIANALITNPEFLILDEPT
                     NGMDPAGMAEMREIIQRLVKERGITVLLSSHLLDELSQIATHYGILHEGHLIKELSKE
                     ELAQESRQFIKIDTSATEQAVTVLDSLGYRDYFVQSSRVIQLFEGIDQVAAINQALVE
                     AKVPVDGIHLVGQKLEDYFLQLTGGNPHV"
     gene            complement(20316..20570)
                     /locus_tag="ACG842_001697"
     CDS             complement(20316..20570)
                     /locus_tag="ACG842_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0248242.1"
                     /translation="MSTITTITMVASPLGLALFGALFDWKTTQPRLRDSLIFLGGSLA
                     IVILITGLVKLLKLDLKEAKIFSEEEYRETTLVEKKESGN"
BASE COUNT         6296 a         4371 c         3528 g         7039 t
ORIGIN      
        1 aaaaaataag ctctgaaaaa ataattttcc cgagcttatt tttcaacaaa aacagcttca
       61 aaaacaaagt cttgatataa atgtctcgat accgaatact cgaaaggttt accagacgat
      121 agagcaacaa ctttgtctat ctcaatcata aattcacctg gatctatttt taatattttt
      181 gcgtcaaatt cattcgcagt atctcctttg atacgtatgt gcgaagtctt tatttccaga
      241 cccagttcat ttgtgatata agcatataca gatttcttta aatgttttgg ttccaagcct
      301 ggtatgactg ctaatggcat gaatgtttgc tcaatcgagt atggttgaca atcaataaaa
      361 cggcttcgta caatattata tacatagtct tttacactga tacttaattt atcagctact
      421 tttttcgatg gaagttcaat tgagaaagta tggatttctg aatctacgtg ttcttcacca
      481 accacgctaa acaaaccttc gtttggacca agaactcgtg agttatcaat tggtgttctg
      541 acgacttcag tcccataccc acttctactt cgcaaaactc cttcggaaac aagtaagtct
      601 acgcctttct ttactgttaa cttgcttgta tgataaataa ctgccaattc atctcctgta
      661 ggaagtttct caccaatttt atactctcca tttacaattt ttttctttat atctaagtaa
      721 atttccatat acttcgcaat ttttttcaat ggtgaccctc caatcaaaaa catatcttca
      781 tctatacttt aacataaata aaccgataaa atccacatca aaattataca cacaataaaa
      841 aagatatata tatttaaaca aaactattta ctttaagttg taagcggtgt tatcattaag
      901 tggaaacagg aggtaaaaaa attatgacaa ctatgaaaaa gctaactttt ccagatgatt
      961 tttggtgggg ttccgcttgg tcagctgagc aagcagaagg aaaaggagaa acaggaaaag
     1021 cagagactat ctgggatcgc tggtttaaag aacaacctta ccggttttat caacgaatcg
     1081 attcctctat tacaaccgat cacatccatc aatataaaga agatatagaa ctaatgaaaa
     1141 aaactgggca taactccttt cgcgtttcta tttcttgggc acgaatgttt ccaaatggcg
     1201 gtaccggaaa agtaaatccc aaagccattt ctttctatcg aaaattattt tccgaaatga
     1261 ataataacgg aatcaaagta tttgccaact tatatcattt tgacatgcct gctactcttc
     1321 aagataaagg aggatgggaa tcacgtgaag tagttgatgc ttatgttcat tacgctgaaa
     1381 cttgttttaa agaatttggt gacttagttt gtcattggtt taccttcaat gaaccacttg
     1441 ggcctatttt aggtacttat cttgaagatt ttcattatcc aaacctcatt gactttaaac
     1501 gcggagcgca agcagcattc ttcactatac ttgcacacgc aaaagcaata caagtattta
     1561 aagaactagc tcttcctagt aaaataggcg ttattctaaa tttgagtcct acttttcctc
     1621 gtagtcaaaa tcctgctgat gtgaaagcag cagaaatcgc tgatttattt tatacgcgga
     1681 gctttttaga tcctatggtt aaagggaatt tttctaaaaa gcttatcctg ttattacaag
     1741 aatataatca gcttcctgaa gattatacga aagaagatct tgtatgtatc tacgaaaata
     1801 ccgctcaaat tcttggacta aactattacg agccacgtcg cgtcaaatct cgttcttcta
     1861 tggtggatac atctagtcca tttttaccag agtggttttt cgaaccacat atcatgcctg
     1921 gacgacgaat gaacgaatac cggggatggg aaatttacga aaaaggaatt tatgatttgt
     1981 gcatggatat caagaaccat tatggaaaca tcgaagcatt tatctcggag aacggaatgg
     2041 gcgttgcgga tgaagaacgt ttcatggatg atacagggca aataattgat gattatcgga
     2101 ttgagtatat caaagatcat cttgcttact tatggaaggc aataaatgaa ggatgtaaca
     2161 ttaaggggta tcatctttgg acatttattg attgctggtc atggatcaac gcctataaaa
     2221 accgatatgg gctggtttca ctcgacctta ctactcaaaa aagaacaatt aaaaaaagtg
     2281 gcgaatttta caagcaatta agtgatacaa atggatttag atacgatgta gacttgttag
     2341 ttgaataata ttctaaaatt ccacttgaag tgaacacatt agaaaacaag aataatgaga
     2401 aataccaata acaaatactt tttcttcata cttcatttat acttgtcagt ataagagact
     2461 gtgacaaatg tagtgatcct cagaagttag gtctggaaat ctaacttgag aagatttttt
     2521 taaactacgt ccagtctttt ttatttgttt aaaattgaag ttgacaagtc ttcattaaaa
     2581 atttagaata ttcacaagtt tgacgacaat tatgaaaatt tatgtcgtgc taaaggagtt
     2641 ttgttattgg atgaaaagtt agatataaaa actgcgatca aagacacgtt acctaccgtt
     2701 tttggttata tcggtattgg acttgcattt ggtatcgttg ggaaagctgc cgggtttcat
     2761 ccgctggtcg tcacgttgat gtccctgctg atctatgctg gttctgccca atttatcacg
     2821 gtcagcatgc ttgctagcca tagtccactg ctctccatcg ttctctcgac ctttctagtc
     2881 aattcccgaa tgattctgat gagtatgacg attgctcctt atttcaagaa aaatcggctg
     2941 cttcaaaatc tgctgattgg cacattgctg acagatgaaa gtttcgcact gggaatgaat
     3001 aaattgaact atactggaca aaagttaaac tttcgttgga tgaatacagc taattggatt
     3061 tcttatttga cgtgggtggg ttcttctctt gttggcgcac ttttagggaa ttttattact
     3121 gatccgaaaa agtttggttt ggatttcgcc atcgtcgcta tgtttatcgg gttactttat
     3181 cttcaagtca tttctgatag aaacacctct aaacgattac agttgatcct gattggtctc
     3241 actttgattc tggtatatgt cggactgatt tttatcccaa gtaacttagt gattttagtt
     3301 gtaacactga ttgggtgcgg attgggggtg tggatcaaac atgccttctt ttgagtttac
     3361 attgctgact attttaggtt gtactttagc aacttggctt tctaggatcc taccatttgt
     3421 cttactgaaa aaattcgatt tgccgcaacc gttgcttgag tatttaagtt ttgtcccgat
     3481 cgtcattatg tctgctttat ggttcagcag tctatttacc caaaatatcg gacatctgcc
     3541 gcagatcaat ctcgaaaatg ctttggcttc agttcctaca cttttagctg ctattctttc
     3601 aaaaagctta ttggtgatcg tcttggtagg aattctttcc ctttcgttga ttcgcttatt
     3661 ataaataaaa aaacaagagc ttatggcaaa ttactgtcat gcgctcttgt ttttatcaaa
     3721 gtcaaataag aaaaaaatcg gcgtattctt gcttatccgt tgaacggacc actaacaacg
     3781 acttttccta atatatgtcc tgtttctacg tctttggttg cttgtttcaa gttcttcgca
     3841 ttcatcccat ctgaatagac cttgctcaat gtactgcgga taatcccttc atttgctaaa
     3901 gcagcaatgt gcgctaaagc ttctccttgc gtttcgattt catagttata gtctgtttta
     3961 gcgaacatat actcccagtc gaagctgata gattggtttt tccaaacacc tatatctaac
     4021 ggctctttga tccctacgat cgttcccaca tgtccaaatg gtcggacaag gtgtttgatt
     4081 tgatcaaagt agcgggtaat atcgaataat acagcgacat actctacttg atcgattcct
     4141 aattcacgaa aacttttact taagtcgtga tgataatcga ttggatagtc tacacctgtt
     4201 tttttcagcc attcaaaatt cttcggactt gcagtcgcgt agacttctag gccagcccaa
     4261 tgcgctagtt gattcaaaat cgaaccgacg cccccagctc cgttgatcac taagattttt
     4321 ttcccgcggt tggcattttc ttctggagtc agaccaaatt tttcaaataa caattcataa
     4381 gcggtcaatg aggtcaacgg caaggctgcg gcttcttcat ctgaaagatt ttttggtgca
     4441 agagcgacaa tacgttcgtc aaccagttgg tattcttgat tgcttcctgc tcttgtcgtt
     4501 gtacctgcgt aaaaaacgcg atcgccgaca gaaaattttt gcacttcatc acctacagca
     4561 acaactttcc cgactccatc aaaccctaag atacgtaaag catttcttat cccgttatct
     4621 tggcgaagtt tcgtatcgac aggattaaca gatactgcgt tgacttttac cagaagatcc
     4681 cgtgaactgg gtacaggtat ctgttctttt tcatcaataa agctttttgg atcatcgatc
     4741 gacaaccctt cataaaaacc gactaccttc attaattcct tttctgacat acgaattcct
     4801 ccttagcttc atcaagttgg agcatccata cagttttcat ttcaatatat atagtatgct
     4861 atttgttcca cttttattca tcataattat tctcttactt aaagtaaact atttgcactt
     4921 ttttggttaa aaaagaggtt gcgacaagac ttttgtcaca acctctttta aacggtgttc
     4981 aatcaactga ccctcggtat tcgctcaaaa aacgacaaaa tatgaggagc tatttttgtt
     5041 tggctgttct tattacttga tgcaggacag ctttttcacg acctctaatt cacggtgttc
     5101 aagcaagaaa tcattgttca ctgaaaagcg ttggatgttc tttttcaaag gtgtctaaga
     5161 tctctcgtac ttcatcagta gatttcgtat tcatcaactg aactcgcaag tcatttgctc
     5221 ctggaaatcc tttgatatag attttaaaga aacgatgtag tcctgtgatg gaacgcggga
     5281 tgatttctga ataatgatct tgcagatcta gttgcagacg aagtagtccg atcagttctt
     5341 tttctgtatg ttccctcggt tctttttcaa atgcataagg attcttgaaa atcccccggc
     5401 caatcatgat gccatctacg ccatattttt cggccaattc ttctcctgtc tggcgatctg
     5461 ggatatctcc gttgatcgtg atcagtgttt gcggagcgat ttggtcacgt aaagcaatga
     5521 ttttagggat gacttcccaa tgtgcatcta ctttactcat ctctttacgc gtacgcaaat
     5581 ggacagaaag attggctata tcttgatgaa gtaggtgact gatccattct tccatctcag
     5641 acatttccgt atagccgata cgcgtcttga cgctgacagg caaaccgcca gctttagccg
     5701 cttcaatcaa ttgtgccgct acttctgggc gaaggatcaa gccactgcct tttccacgtt
     5761 ctgctacatt agggactggg cagcccatgt tgatatcgat tccttggaag cccatttctg
     5821 ccatagcaat gctcatttct ctaaaaaatt ctggcttgtc tccccaaata tgggcaacca
     5881 tcggctgttc atcttctgta aaggtcaatc ttcctcgtac actgtctttt ccatctggat
     5941 gacagaaact gtctgagttt gtaaactcag taaagaagac atccggtgct gctgcatgct
     6001 ttacgacatg gcgaaataca acatctgtta catcttccat tggtgctaaa acaaaaaaag
     6061 gctttggcag ctctttccaa aaattagtag tcatttattt gttactctcc attctatcaa
     6121 gcagtgaagt cgcaatcttt ctcaaaaaca ttgtcattat actaggaaac aaaaaaagaa
     6181 gcaaagtgaa cagcttaaaa gatgtttctg attgagcaaa aaaagactgt gacaaaaatc
     6241 ttatcacaat tccttttccg gaataaatgg tgttcaaaag cttgcatctg cggtaataag
     6301 tccaaccaaa cgaaaaatag gaggagctat ttttgtttgg ctgttcttat tacttgatgc
     6361 agaacagctt tttcacaacc tctccactaa acggtgttca atcagctgac cctcggtatt
     6421 agctcaacaa acgacaaaat atgaggaact attttcccgt ttgtttctct aatactcagg
     6481 tcaaaacgct gatttcacaa cctcttttct aacttctcaa tcctcttcct ttattgatca
     6541 aataccaagt aacggcgtac aaaacaacga tgaatagcac aagtacgatc atcgatgttg
     6601 ctactggcac atcgattgtt ccaaggaatc cgtaacggaa accggagatc atataaacga
     6661 tcggattgac tttcgaaaca gcttgccaaa atggcggcaa catagagatg gcatagaaaa
     6721 cgccacctag ataagttaac ggctgcaaga caaatgtagg cacgatcgat acatcgtcat
     6781 aagactgagc aaaaatcccg ttgagcagtc ctgctaacga aaacaagatt gccgtcatca
     6841 ataatgtaat gatgaccatg aaccatgaat acacatgaag tggtacaaag aacaaggaaa
     6901 tgatcgttac gagagagccg acgagaatac tgcgacccac tccgccaatc acaaagcccc
     6961 aaatgatcac atgcgtagga acaggcgcta ccaagatttc ttcgatgttt ttctggaatt
     7021 tttgagagaa gaaggacgaa gaaacgttgg catacgaact agtgatagca gacatcataa
     7081 tcaaaccagg cacgatgaat tccatatatg aaaaaccacc catgtcaccg atccgaccac
     7141 cgatcatttt tccgaaaata acaaagtaca aggatgtcgt gatcacaggc ggaaccagtg
     7201 tttggaccca gatacgcaaa tagcggttcg tttccttgat agctaaactt tttaatgcag
     7261 taaaataaag attaaacatt tttctcctcc gtatgattat tttcttctgt gattttcaaa
     7321 aacagttctt ctaaacgatt cgacttattc ctcatagaaa gaactttgat tccttgtctt
     7381 gtcagctgat cgaagattcc gttcacccct tgatttcgtt caacttcgac agcgattgtc
     7441 tgttcatctt caaatgtaca agcgtatcct tcaatcacag gtttggtctc atatggcgct
     7501 aaatcaaaga taaatgtttc aaattgcagt ttggatagaa gctctttcat actagtattc
     7561 tcgatcagtt cacctgattg gatgatcccg atgttgcgac aaagcatttc agcttcttct
     7621 aaataatgcg tcgtcaaaat gatcgttgta ccattttcgt tcaattctcg gagaaactcc
     7681 cacatctcgc gtctcaattc aatatcgact cctgctgttg gttcatccaa gatcagcaaa
     7741 cgtggttcat gcatcaatgc cctagcaatc atcagtcttc gcttcattcc cccggaaagc
     7801 atacgtgcac ggacatttct cttttcccaa agattggact gcttcaaata tttttcgctg
     7861 cgtttcatcg cttctttgcg agagacaccg tagtagcctg cttgattcac gacgatttgc
     7921 tggaccgttt caaagggatt gaaattgaac tcttgcggga ctaatccaat ctgttgcttt
     7981 gctcggacta gatccgtatc cagatcataa ccgaagacat tcacttttcc agatgtttta
     8041 ttgactaaag aagtaatgat tccgatcgtt gtagacttcc cagctccatt tggtcctaat
     8101 agcgcataga aatctccttc tttcacggaa agatcgattc ctcgcaatgc ttcgacccca
     8161 cttccataaa cctttttcaa attatttatt tcaagcgcat ttgccattta tttcctatct
     8221 ccttatagac atgattcttt attgtatcat ggaatacagc agatgaaaaa acaacttggt
     8281 tccagcgaaa aatcaggttg ctgggctcag gaaaatctcc taatgaaaga catcaatcgg
     8341 acgaagtcag acaacctttt cagaaaaaac aaatcgaaga taaaggatta ctattcatat
     8401 gtgcaacaaa aggagcgtga gacaaaagta aagaacactt ttgtctcacg ctctaaacac
     8461 gtataaacgg cggaagcaaa agccaagatt tttgatccaa taaatacttt cgatcacttc
     8521 gcctatccca atacatgcca ctgccgcttt tcatcaaata acgcggcttc cgtttttgcg
     8581 gtttctacta aagtctctgg gtaatccaaa actttcagca aggcaatcgc atttcgggtc
     8641 cttgaaggtc cttcttttaa tttgaaatca aaactgattc cttttccttc tgttacttgt
     8701 tcttcaaagt ggacattctc acaataattc tttaaaatct ctgtcaattc gatatcatgt
     8761 gtggcgacaa atgctaaaga tggatactcc gcaagccacc gaacgactga agcggaagaa
     8821 gcaatccgtt cgatcgtatt tgtccctttt aaaatctcat ctacgaagca gtagcagcgt
     8881 tctttggaag ccacttggtc caacaaacgt ttgatcgatt tgatttctga aacaaaataa
     8941 ctgtcgcctt caaataaatc atcttcgatt gccatggaag tcaacacatg tccaggttgc
     9001 agggtaaact gttctgcaat cgctgtttgg atcgtctgtg ctagaataca gctgatggct
     9061 atacttttga catacgtaga ttttcctgat gcattggaac ccgtgactaa tgtattcttc
     9121 tgccagttga ccggattcac taccgcttct tttaacaatg gatgatagat atctttcgct
     9181 gtcacaccgc cctcagtaaa ctctggtcgg caagtgatgg gcatataagt acggtaattc
     9241 aatattgcag cggctacttc taattctccc agcaattccc acagcttgat ggcttcagtt
     9301 gaatgctttt ctaatttagt gagaacgaaa tgataggaga taaatggcag catgaacatg
     9361 atgttgacat attcaaaaat caactctgct tcactcccac tcttcatacg aaatgaaaaa
     9421 cctaatttag ggatgtctct taatggttta gtttgctgtt tcagctcttc ttgaagtggt
     9481 gtcggcagtt ttgctagctg attggcggct gaaatcgttg aaaccaaata actcatgcta
     9541 ttcagctctg tttctagtgt ttgtttcttc actagataat agaccgtatt gaatactaac
     9601 ccaccgattg ccaaatagat tcctagtact tgtcctaaaa gaagcaaacc caaacccacc
     9661 agcggaagca agccaagaaa aataaataaa cctacgcgtc caatcttttt actttttcca
     9721 tccgctagat attgttttgc aaaattatga tctttttttc ctaaacgagc gaattgatat
     9781 tggatttttt ctctcagttg aggattttcc tgataaaaag caatcagttt ttctaactgc
     9841 tgatcttcac caaaatcaaa gctgcgtaaa cgttgataca acgcttctga acctacacta
     9901 gaataggtac tgttgattcc ttcgaatact tcgaacatat ccaggtcata ccaagtaaga
     9961 tcatcgattt cgctatccca tgaacggaac ttcttctccg tgagccatgc ttccttcaag
    10021 ctttcttctt tatcaaatct cggttgataa ggaatttttt cccatttgct acgaactgtt
    10081 tcctttaatt tcaaacgtga aaaaatgtct aaagcaacta ataaaataac agctgcaata
    10141 ataattaaaa cgataaattc tctttgaccc atatccctct ccgttccttt atttgttagc
    10201 tttagtatag cactgttgcg ctatttgaaa agagggtaaa aaaacacctt tcaaaataaa
    10261 aggtgttttt ttcttgtata ttacatattt atctctcatt tatcaaaatt gacaaataat
    10321 catagggagg aattgttgtt aattcttgtt tattctccag tgcattgaca actgtcccaa
    10381 gggaaaggat agcctcaggt actatttttt cttgttctgt tggattagct aagcgaagaa
    10441 caaatccttg ttcatgaaaa taagaaggat aaatcgatga tacaattaag tcttcaggta
    10501 attcaatcaa tgaaaattta cgaggaacat caagttcatt ttgtaatggc caaatcttgt
    10561 tatctaacct gtgaataaat acattcagtt tctgcttttg ataagaaata gattgttcta
    10621 accgtttttc tgcctgtttt gccactttga actcattaaa tcttccttct tctacattaa
    10681 atccaaaagt gactttatac tcacctatct cttgagctag cggtgtttcc atcatgacat
    10741 gcccttcatt tgtcgtatct cctgaagctc gcccaggtcg ccaagcaaga ttaggttttc
    10801 caagttcccc tgttgttgaa aataaggtta tgattaattg attttctata cgttcatact
    10861 cttttaaacc atctgcataa aacgtaaaat aatgatcatt ttctgccaca gagacactct
    10921 tatcgaatat ttctaaatta acaggtttct ctaccatagt ttctgaccaa ttcggaggaa
    10981 tgacatcagg ggtattttca ataaatccgt tttgaatttg tgcaattgat ttttcagatt
    11041 tatctaaaac attaattgct aagcgcatac gatgcgagta aacagtatta tgaattttga
    11101 gtgtgccttc aacgatttga ctttctccca gactgaacga tatttcataa gatatttctt
    11161 ccatttcggg tttttcagat aaacgatctt ttaaatcttt aggaagttga gcttttccgt
    11221 aaacaactaa tgtttcttgt aaagaatctt tatatacttt agctgtttct aattttagag
    11281 atagttctgt atctccttct aatggtgaat aatcatacgt atcgccatca tttgctgaat
    11341 cgatcaaatg aacgaaatca agatattgtc tatctccaac tatcaaattc aattgcccat
    11401 ctttaaaaga aagtttatat ttattgttac ttatctcagt tttgtctatc tcgattgctg
    11461 tttctaattt ttcattattt ttctcaaatt caacaacagt gtatcctagt gctggcacat
    11521 ttacatttat ttttatgtca agttcataat aagctggttc atcaaaatat tcgaaacctg
    11581 tagcaatcat ccgataagca tttttccgtt ctggaaaata aacttctttt tcaatgatat
    11641 tatagggact acctttgaag tgtattttct ttgaatcagc tactacatgc acgattttcg
    11701 atccactata attctcagtc gttgtgttga aaacaaggac ttcattcgat tttagcttta
    11761 atgcatcagc catccgtttt acaataaggt tttcgattcc gtcagctatc tcattagctt
    11821 ctttgattcg atggaaaata tcttccgcaa cattatctga aacacagcca ccaatactat
    11881 catgagcctg gttttgcaaa atctttttcc ataaccgacg aagtaggcct ttactgatat
    11941 ggataccatt tctttctgca atcagcatga gcggctcaac tcgttttaga attttttgtt
    12001 ccaatttaaa attttctatt ttcatttttg ttcttactga gccaatagaa cgatgaacac
    12061 gagagtaaac cggctgacga agttcacctt tatattcagg taattctttt ttgtttctga
    12121 taacttctac ataagaagga taatctgaaa taatattgtg gaatttactg tatttattca
    12181 acttctctac gacttgatca aaatttaaaa tcattgactt ttgatctaca cccgaaggta
    12241 ctaaaatatc tttgtttttt ccttgcatat gaagcatttc aactgcttca tccaactttt
    12301 cttttgcaaa ttcaggaata tctttaattg cgtctaaggt catcattcca gtcatataac
    12361 caaatggaaa attcatggca taaacaaatt tatctcccaa tcctttccag ataaaataag
    12421 gagatggaac catcgtttca aagtttaatc ctcgccaaca catgaaatta tcgatttcta
    12481 cttgatgcaa aagtgtaggc aaattcgcat tgaacccaaa cgtgtcaggc aaatacccta
    12541 ccatcattgg atctccatat ttaaggatcg tatcactgat tccaatcatt aagtttctta
    12601 aaattgattc agcatccact aataaggcat ctgtttgtgc ataccacggt cccacgaata
    12661 gttgtcctgt tttcatcagt cgttggataa ccttaagctt ttgcggattg atctccaaat
    12721 actcatcgac gattgaagtt tgtccatcta aacaaaagtt tactttaggg ttcctttcca
    12781 attcatctag aacttcagta aagacctgat cagacaatac gatggcatct tcatttgtaa
    12841 aataccattc tctgtcccag tgcgtgtgtt gaattatatg tgcatttacc atatcagatt
    12901 ctcctacttt ccttcttcta ctgaatttac ttgttgttct tttttatact tgatggattt
    12961 tagtgcattg accattaacg ctgtaaccac tcccccaata acaattgata ctaaatataa
    13021 aggtatattc gtaacaaaag gaattgccat aaaggttgaa agcattgttt cttgagtaac
    13081 accaaaaaat ccatttagac cacatgctac tgcagtgccc accacgatgc ttggtattac
    13141 tcgtaaagga tctttcacgg caaacgggat cgtcccttca gtaatcccca caaggcccat
    13201 aatcatagct gtctttgcat tatctcgttc cgattcatca taaagttttt tgtttaaaat
    13261 tgttgctaaa aacattccaa atggcggaat agcaattcct aataacatgg ctgtactagg
    13321 tccaaagctt ccgcttgaaa aagaagcaac cccaaaagca tatgcgattt tattgatagg
    13381 tcctcccata tcaaacgcca tcattcctcc taaaatagcc gacatcaaag cagcatttgc
    13441 tcctgacatg ccttctagcc aattagtcag tcctgttgtc attgcttgaa taggcgtagc
    13501 tagtacatac cataatatga ttccaacaac taatgtagta acaaaaggaa tcagcataat
    13561 tgattttaga ctaataactt gcatcggtac ttttactttt ttcaatgcat tagctaaata
    13621 gccacaagct aatccagcaa ccattcctcc aataaatccc ataccaagat tttgacacat
    13681 catcccagtg ataaaagctg gtgctagacc gggacggtca gcaattgaat atgcgatata
    13741 ggctgaaatc atcggtacga taaactgtaa tcccgtctga ccaatcattc gcaggactcc
    13801 ccagaatgta tccgtctggt cccaaacacc tgtgccacca aaaatgactg caatagaagt
    13861 tagtaaccct gctgctacga caagcgggat catataggaa accccacttt ggaaatgatt
    13921 tagaatttct cttccttgtt tcttgaattc cattagtaac tcccccacga tatatttttt
    13981 atttgttaat taatgcaatt gcttcatcaa tcgtctcttc accatctttt actacctctg
    14041 aggtccctac tgttaaaatc ggtttaccgt caaagcgacc tcgttctgaa acatttgtat
    14101 ctactgcaaa tatgacaaca tctgcctcgg aaacatccga aattgaaagt ttgttttcta
    14161 tccccatagc cccctgtgtt tctactttaa tatctaaccc tttcttcttc gcaaactttt
    14221 ctaaattagc ttttgccata taagtatggg caatccctgc gatacatgct gttactgcta
    14281 ctatttttgt catatgaaat tcctcctttt aaaatgatag agcttttacc aattcttttt
    14341 tatttctcga atcttttatt cttttaacaa actcgtcatc catcaatctt ccagataatt
    14401 ctgctaaaat acgtaaatga ttagtacctt tatcagtgtc ttttattgct aacaaaaaga
    14461 cgagatcaac tggttgatca tctaatgcgc cccattcaac agaccgcttt aacttggcta
    14521 cagccactgt tgattcaatt actgctaggc ttttgccatg aggaatggcg atatttttcc
    14581 caattcctgt ggtcatatgg ttttctctgt caaatacgtc tttttgaaat tgtttgtggt
    14641 cagacacaaa cccattagtc tctaacaatt tcgataattt ttcgactaca ctttttttat
    14701 cagatgcatt taaatctatc actatattat ttatattgat tttttcactg atcatttata
    14761 agccttcttt caatatataa actaattttt caaacgacga actcgttttt aaatttgcta
    14821 ataactcatg attcgaagcg atttcattga atgtttcata aattttattc aattcaggtt
    14881 ttactttttc attaagtgca aaaaagaaaa cagaatgaac aagtgtccca tcccaatcaa
    14941 ttccttctgg attaacgtag acacagatta ctggcttttt tataaaatct acttcgatat
    15001 gcggcatagc aaaatcattc atcgctgttg aagatagttt ttctcttttg attgctccct
    15061 ctagtatccc ttctttcgcg taattttctt ttaccaattt tttggtaatt atatttaaaa
    15121 cgttcacttg attatccttt atttggtggg taaaaaagca aaatcgttta tctaataaat
    15181 ttagaaaagc atcttctgtt tgttcattta actgtagata ctgttttact tttctgacat
    15241 ctgcccctaa catcaaagga ctaacaacaa taacaggaaa atttgtattt tcaatcggaa
    15301 tcgtgcttat aacaagttca tcagtgactt ttctatcttg tagttcattg atgcttagta
    15361 catcttctat ctcaaccata tctgcaaaac cgttatttat tctttgctct aataattttg
    15421 atgttccata tccgctacta caaactaata tcactttcgt tttgtcaggt ttgtaacgat
    15481 ccaaaagtga ttgaatatgg agtgttacgt aagcgatttc atcttcatta aaatgtatac
    15541 agaatctctc ttcgatttcc tctaataaat ctacacttat cataaatgac cttttaaaag
    15601 aatatttgat tttgtccgta taagggttat agatatttac tcccaatttg agtcttttaa
    15661 ctgctgcatt gagatgtaat actagattat tgatcaattc ttcatcagga taaaattcat
    15721 ttaatttaat tcttactaac tcttgtagct caacacccat atctaaatag gaaagcttcg
    15781 tttgatctaa gtcgtgattc atagcatttt tttcaatcga agtaatgtga atgtcaacat
    15841 aagcttgttc atctattggc acaataattg aaaatgcttt ttcaatcatt ttgattaaaa
    15901 taagtgtatt ctttgagatt tcttcttcag aattattact acgtgataag aaattgcttg
    15961 tttcaatcct ttctatcgca atagcaaggt ggatagctaa agtttgaaat tcataatctg
    16021 taaatttcaa ttgtgtctta ttcatgtatc gattaagcaa ttcggctact tttttcaata
    16081 tgtccttatt taagaaatta ttcgcttcac caatgaaatt gatattcaag tctaagagat
    16141 ctgttttact gttagatgct gtaaagccac cccaataata gttcacaact tctgacatca
    16201 accttctttt acttttttca tcagctttga tgcgcacgcc atttcgatct gaaactaagg
    16261 acatcccatg cttgtttagt ctttttctaa catctttcat tgtgctttct accgtcgacc
    16321 tgctcacgta taattcgtca gcaagatttt gaatctttag agaatcatta gtattcaata
    16381 atttagcaaa cacataagtt agacggtcct ccggagagtc tgcgatattg attttatgac
    16441 tggataataa atttcgaagt gtttcttttt ttccaataag cgaaacgcct tttttaggtt
    16501 gaaccaacat ttgtacatct ggcatttttt ggattatttc tttcaaaatt aaaatatctt
    16561 tgctaattgt tttcgtagat acatataatt gttctgataa ttctttgtaa gttgtgtgct
    16621 cattttcaat caaaaagtat agtattttca tttgccgttc gctaaattcg atgcccatta
    16681 tcttcacctc ctgaatacag cttaagtgta caattattat ctctattttc atacagaaga
    16741 aagttcttga ttaatgtaga agtttctaat attcttgatt atttcaaatc aataaaaaag
    16801 cctgcaatat ttatatcaca ggcttttttc tctattaaat atatataaga taatacattt
    16861 ttctactagt tctcactaat cttgtataat taaaccctcc gcaacttgac ggagatggag
    16921 aagatgcccc cgtctaatct agcggtcatc tctcctccca ttccttccac caattttttt
    16981 gacaggtaaa gcccgattcc gctagattcc gtatttgtgc ggctggtatt ttcggtataa
    17041 aaacgctgga agactttttc aacagaggtt tgcgttcctg gtttgatagg attggccact
    17101 gttaatcgaa tgaatggatc ctcttttgaa aatgtcacct ttgcttgtcc atctgcataa
    17161 cgcaataaat tctgtatgat attttgcagc actcgcagca acatgagcgg atctgctaat
    17221 acagttgtat ttatcgagtg ttcagcgaac gctacctcga tatttttctc agaaaattgt
    17281 tcataaaaag agaaaaatac ttcttccacc tctttagaaa aagaaagcgt atggagatcc
    17341 atttcttttt gcttcgtctc tattcgagca agatcataaa actgatccgt catactgagt
    17401 aatcttgcaa cagattgttt gatgatcgtt aaatacttct cccgtttctc cgtatcctct
    17461 acctgttcca gtaattgtac ataaccgtta atagcagtca aaggagttct gagatcatga
    17521 gaaaggccga cgatcgatag tctgatgttt tcttccattt cttgaaatgc ttgtcctttt
    17581 tgttcatttt catccaacca tgtattcagt gcatctacta ggtccattag attcttttct
    17641 cgaaattcga ctgaaagacg accaccatat tcttgtcgtt cagaaagagt ggcgattttc
    17701 ttagttaatc tcttgatttc cctttgcagg aagaaaataa aacttcccat aaagataaca
    17761 acgagaagca acagccattt aatcaaatct aaaccccact ccccacaccg tatgaatata
    17821 cacagcatgt gcctttgcta gcttccctcg aagattggaa atatgcatat tcactgtttt
    17881 ctcactatca aaatatggtt cttgccaaac tgtttcataa agatttgctc gcgaaaatac
    17941 ttttttcgga tgttctaaaa aaagcttcag cagatcgaat tctcttcctg tcaaatggac
    18001 agccacacca ttcacattta catccctcga ctctttgtcc agtaagattt ccttgtaata
    18061 gatgcgttgc gacagatctt ttacttggac agaagtctga tgacgaagtt ggatgccgat
    18121 tcgtaacaaa agttcttcga tatcaaatgg tttcgtcaca tagtcatccg ctcctgctgc
    18181 caacaaggaa actttatctc tttgatcgct ttttgctgaa agaacgatga tcggaacatt
    18241 cgatgtctgt cggatttctg ctaacaatgc ttcacctgaa agccctggaa gcatgagatc
    18301 tagaaggatc aaatcgacag gatatgtgct taacaataat tttccttccg tacctgaata
    18361 agcatcaaga agtttgtatc tctgttcaag aacctcctcc agtaaggaat gaacagcttc
    18421 atcgtcttcg ataatcaaaa tagtttccag caggaccacc tacttaatgt cagatttttg
    18481 gaaaataaac attcctagag ctgatgatac cacgattgtt actagactga ccagaatcat
    18541 tttcggtgct tgcgaataag ttgcttgttg cgcaaagtcc gtatacatga gccacgtttc
    18601 cgtaaaccac tccgttagaa attccaagcc tttgattttt gcgaaaacac cgggaatgaa
    18661 ggccgttccc atgatataaa gaaaagaaag aagcaaactg acactggtac ttctagtgat
    18721 gaatgtcaaa gccactggaa cagataatgt ccctaatgtc agcagaagca atacaggaaa
    18781 tacttttaga acataatttc catagttttt gattccctca acttcaccaa atccgaaagc
    18841 aagtgtgtaa gcaagcatgg ctagtcccgt gatcaaaacc acgccaagtg ttgtgatcaa
    18901 aagcgtaacg agaagtttcg acacataata agtcattcga ttccgtccta aagataacgt
    18961 attgcgaatc gtaccgtttt gaaattcttc cccaatataa aagcacgcaa atgctgcgaa
    19021 agccagaaga atccatgcag ccattttttc taccattaaa atccctgttg caccgttgat
    19081 gactgtatcc gttgcttggt ttaattcatt gccgtatttt gtatgcatat accattcgtt
    19141 ccaagaataa aaaagcggaa gtacgaccat cagtcctagt gagacccaca ttttacggct
    19201 cttccatact tttatttttt cagcagttaa tagattaaac atgtggattg cctccagtta
    19261 attgtaagaa ataatcttcc agtttttgcc caactaggtg aataccatcc acaggaactt
    19321 ttgcttctac caatgcttga ttgatggctg ctacttggtc aattccttca aaaagctgga
    19381 taacacggct agattgtaca aaataatccc tataaccaag agagtcgaga accgttactg
    19441 cttgttccgt cgccgaagtg tcaattttta taaattgacg agattcttgt gccaattctt
    19501 ctttcgataa ttctttgatc aagtgtcctt catgcaaaat accatagtga gtggcgatct
    19561 gtgacaattc atctagcaag tggctagaaa gcaagacggt gatgccccgt tcttttacca
    19621 aacgttggat gatttctcgc atttctgcca tccctgctgg atccatccca ttcgtcggtt
    19681 catccaaaat caaaaattct ggattcgtga tcagtgcatt tgcaatagct aaacgctggc
    19741 gcatacctaa agagaaattc tttgctttct ttttccctgt attttccaat cgcatcattt
    19801 tcagcaggtc ttcgatttct ctatccgaca caccgccgtt cgctgcttgg atacgtaggt
    19861 tatctcttgc agttaattct ggataaagag caggggtttc aatcgattga cccatacgtc
    19921 gtcttgctgc ctgcaaatcc gtcgttccaa ataattcgat cgaaccttca tcaatcgaga
    19981 tcaaacccat aatagttctc ataaaggtag acttacccgc tccattttcc ccaatcaatc
    20041 catagatcat tccacgtttg atttcgattg atactttatc taatgctttg gcagcaccgt
    20101 attttttggt tataccagtt gctttcaaga ccgtttcatt tccaaaatcc atacttatcc
    20161 ttctttctta ttgatgagtg aagtatagtt gacgggtata aagaaagcag ccagaaatgt
    20221 ataaagaaag tgtaaatttt ccaattgaat gtcaaaaaaa acaatcggac aatacgtgtc
    20281 tttcctatta tcaggcagtt tgttatacgc ttgtatcagt ttcctgattc tttcttttcg
    20341 accaaagtcg tttctctgta ttcctcttca ctgaaaattt ttgcttcttt cagatcaagc
    20401 ttgagtaatt ttaccagacc ggttataagg ataactatag caagactccc tcccagaaaa
    20461 attagactgt ctcgcagacg tggttgcgtt gtcttccaat caaagagcgc accaaacagt
    20521 gctaatccaa gtggactggc caccatggta atcgttgtta tcgttgacat aagtcgtcct
    20581 tgtatatgcg ttggaacttc tcgctgaagc cagaccatat aaaaagcaaa agaaaacatt
    20641 aatccttaaa aacggactta tgaaaaataa tacatgccgt ttgggattta caattagcta
    20701 ccaaataaga ggttatgaca aaagactttt cacaatccct tcttccgaat aaacggtgtt
    20761 ctcctgccaa ctcctcggtc acaagtcaca atatttatca aacatgagaa gcaatttttg
    20821 taaattcctt cttatttctt gaagctgacc acttctgtca caacctcaga agcggataaa
    20881 taatattgtt tgatgatact ttatcttttt cccgtttgtt tatagatcga atgatattat
    20941 agaccaagaa tcctacctat taacatagct aaaaacacaa aaaaggcact ttccaaattg
    21001 tggaaagtgc cagaaaagtt aggattaagg taactgcgct cagcattgct tcgcccagta
    21061 cgattctcgt aactcgtgaa ctcgaacgat aatcgcaatt aacgttttga gaactgtgaa
    21121 gctttacggg ctttcttaag acctggtttt ttcttcgtat cattgaataa gtttgaaaaa
    21181 ccttgattta taaaggtttt cccgtaaata tattttatta tatttgatta aatt
//
LOCUS       DAZKLM010000052        20984 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3764
            SAMEA115706913-rid22582563.denovo.053, whole genome shotgun
            sequence.
ACCESSION   DAZKLM010000052 DAZKLM010000000
VERSION     DAZKLM010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706913
            Sequence Read Archive: ERR13196647
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20984)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20984)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287823.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287823.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,004
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,849
            CDSs (with protein)               :: 2,849
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 41 of 96
            Pseudo Genes (incomplete)         :: 52 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 15 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20984
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706913-rid22582563.denovo.053"
                     /isolate="VRE3764"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..563)
                     /locus_tag="ACG842_001698"
     CDS             complement(<1..563)
                     /locus_tag="ACG842_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF443 family protein"
                     /protein_id="HFQ0248243.1"
                     /translation="METVRLWIFMKNFRSALRYVILESKSGYYLIDTDHYTFWGGYLF
                     PIFNWIIPHKVMKINLPAEEINRLILNKDLQRKREKEHSNVAWIGPAFAVVTSSIVGP
                     MLESIYSDFSIGIRYLFVTLSIAVLLCIKGYVSKKNKDILTLIGVNNLSSTKIILFPE
                     SIKEGSRLLIIFIMLILFSVLFGVMFI"
     gene            complement(750..1400)
                     /locus_tag="ACG842_001699"
     CDS             complement(750..1400)
                     /locus_tag="ACG842_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0248244.1"
                     /translation="MFIKGGQKMCFFTTFTIIMIAVLFIFLDIAKRNTAFLLYRVLLR
                     AGLITFISIVGFFLFTVIVFIWRTTAPPLPEITYGEFPFSLEYEIDEERILIEDTVIA
                     EFIGSFRGNVTTQPRRSWSIKLLNNSGELKAFHYEFLIIESEDVTITLSSGSAGYFMN
                     DGGSKPQLTVRDRIAGFMHFSHYDLEEARKILAEYGITLINWEYAPPIRNFFNDYY"
     gene            complement(1943..2878)
                     /gene="whiA"
                     /locus_tag="ACG842_001700"
     CDS             complement(1943..2878)
                     /gene="whiA"
                     /locus_tag="ACG842_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0043937 - regulation of sporulation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HFQ0248245.1"
                     /translation="MSFAADVKKELTGLEVHREHAKAELAALLRMNGSLGIVNQQFIL
                     NVQTENAAIARRIYSLLKDHYQVRSELLVRKKMKLKKNNVYIVRLKQGTKDVLMDLDI
                     MDGMMFNGHVSDEIMGNSQKMRSYLRGAFMASGSVNNPETSRYHLEIFSIYEQHNQDI
                     CDMLNYYGLHARTLERRNGYISYLKGAEKIADFLTLIGATNSMLRFEDVRIVRDMRNS
                     VNRLVNCETANLNKTIDAASKQIENIELIEAKVGLHALPEKLQEIAELRLQHPEVSLK
                     ELGEMIPSGAISKSGINHRIRKINEFAEKLREEAV"
     gene            complement(2912..3910)
                     /gene="yvcK"
                     /locus_tag="ACG842_001701"
     CDS             complement(2912..3910)
                     /gene="yvcK"
                     /locus_tag="ACG842_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine diphosphate-N-acetylglucosamine-binding
                     protein YvcK"
                     /protein_id="HFQ0248246.1"
                     /translation="MKMKTYRIRKPKIVVVGGGTGLPVILKSLRNQGADITAVVTVAD
                     DGGSSGAIRESIAMAPPGDLRNVLVALSDMPQFYEDIFQYRFKKEDQFLANHTIGNLI
                     IAAVSEMRGSTYEAIQLLAKMMHVDGHVYPSSETPLTLHAVFKDGTTAVGESKIAINR
                     KTIDRVYVCNTNDKSEAKAARKVVSAIMDADMVVLGPGSLFTSILPNLVIPEIGEAML
                     KTTAETVYICNIMTQKGETEHFTDADHIRVLHKHLNQPFIDTVLVNTEKVPEDYMDPE
                     IYDEYLVQVAHDFNGLRNEGCRVISTDFLELKDGGVFHDGDKVVEELFRLVFGSKY"
     gene            complement(3907..4791)
                     /gene="rapZ"
                     /locus_tag="ACG842_001702"
     CDS             complement(3907..4791)
                     /gene="rapZ"
                     /locus_tag="ACG842_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HFQ0248247.1"
                     /translation="MTDNLQLVIITGMSGAGKTVAIQSFEDMGYFCIDNMPPRLIPKF
                     WELIKESGKVTKMALGVDLRSRSFFEEIQNMLIEIENTNFIDTSILFLDASDEELVAR
                     YKETRRAHPMAMDGLITEGIRKERAILDDLKAQASVIIDTTTLTPRQLREKINESFKS
                     KGDQGFRIEFVSFGFKYGLPIDSDIVMDVRFLPNPHYIPELRPLTGKDQPVYDYVMSF
                     DETQSFYHKFLDLLETVLPGYIKEGKSSLTVAIGCTGGQHRSVALTERLANALGKNYK
                     VNVTHRDKDKRKETVNRS"
     gene            complement(4926..5657)
                     /locus_tag="ACG842_001703"
     CDS             complement(4926..5657)
                     /locus_tag="ACG842_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate racemase"
                     /protein_id="HFQ0248248.1"
                     /translation="MENFFSILGGMGTMATESFVRLINHRTKATKDQEYLNYVLFNHA
                     TVPDRTAYILDRSEENPMPFLLDDIEKQNLLRPNFIVLTCNTAHYFFEELQAATDIPI
                     LHMPREAANELVRQHTTGRVAILGTEGSMKAGIYEREVKNLGFETVIPDTALQEKINY
                     LIYHEIKESDYLNQELYYEILEEAVERLNCEKVILGCTELSLMHEFAEDNHYPVIDAQ
                     SILADRTIERALAERSEALDTASEK"
     gene            complement(5661..6926)
                     /locus_tag="ACG842_001704"
     CDS             complement(5661..6926)
                     /locus_tag="ACG842_001704"
                     /EC_number="6.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aspartate ligase"
                     /protein_id="HFQ0248249.1"
                     /translation="MMNSIENEEFIPILLGSDMNVYGMARSFNEAYGKICQAYASDQL
                     APTRYSKIVNVEVIPGFDKDPVFIETMLRLAKERYSDKSKKYLLIACGDGYAELISQH
                     KQELSEYFICPYIDYSLFERLINKVSFYEVCEEYDLPYPKTLIVREEMLVNGHLEQEL
                     PFEFPVALKPANSVEYLSVQFEGRKKAFILETREEFDLILGRIYEAGYKSEMIVQDFI
                     PGDDSNMRVLNAYVDEDHQVRMMCLGHPLLEDPTPASIGNYVVIMPDYNEKIYQTIKA
                     FLEKIEYTGFANFDMKYDPRDGEYKLFEINLRQGRSSFFVTLNGLNLARFVTEDRVFN
                     KPFVETTYGTNQSDKARLWMGVPKKIFLEYARENEDKKLAEQMIKENRYGTTVFYEKD
                     RSIKRWLLMKYMFHNYIPRFKKYFHVKEG"
     gene            complement(7189..10005)
                     /gene="uvrA"
                     /locus_tag="ACG842_001705"
     CDS             complement(7189..10005)
                     /gene="uvrA"
                     /locus_tag="ACG842_001705"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HFQ0248250.1"
                     /translation="MANDKITIHGARAHNLKNIDVTIPRDKFVVVTGLSGSGKSSLAF
                     DTLYAEGQRRYVESLSAYARQFLGQMDKPDVDSIDGLSPAISIDQKTTSKNPRSTVGT
                     VTEINDYLRLLFARVGHPICPNDHIEITSQSVDQMADQVMALPERTKIQVLAPIVVKK
                     KGQHKKIFERIQKEGYVRVRVDGEIYDLSEVPELEKNKKHDIAIVIDRIIVKEGIRSR
                     LFDSLEAALRLADGYALVDVIGEEEMLFSEHYACPYCGFTVGELEPRLFSFNAPFGAC
                     PDCDGLGVKLEVDQDLVIPDRTKTLSQGAIVPWNPISSQYYPQMLAQACESFGIDLDT
                     PFEELPEEHQEIILNGTDDLFHFHYENDFGGVRDVETTFEGVLKNIERRYHDTNSDFT
                     REQMRLYMTELTCQTCKGYRLNPQALSVQINGTNIGQTNELSIQKAVDFFSNLTLSEQ
                     EKVIAKPILKEVNDRLSFLENVGLDYLTLSRASGTLSGGEAQRIRLATQIGSNLSGVL
                     YILDEPSIGLHQRDNDRLIGSLKKMRDLGNTLVVVEHDEDTMWAADYLIDVGPGAGEQ
                     GGQIVAAGTPEEVANDENSLTGKYLSGKKSIPVPKERRKGNGKAIKITGASENNLKNI
                     SVEFPLGEFVAVTGVSGSGKSTLVNSILKKSLAQKLNKNSAKPGKFKTISGYESIEKI
                     IDIDQSPIGRTPRSNPATYTSVFDDIRGLFAQTNEAKMRGYKKGRFSFNVKGGRCEAC
                     RGDGIIKIEMHFLPDVYVPCEVCHGKRYNSETLEVHYKGKSIADILEMTVEDAVEFFK
                     HIPKIHRKLQTIVDVGLGYVTMGQPATTLSGGEAQRMKLASELHKISNGKNFYILDEP
                     TTGLHSDDIARLLHVLQRLVDAGNTVLVIEHNLDVIKTADYIIDLGPEGGEGGGTILA
                     TGTPEEIINVKESYTGHYLKKIMV"
     gene            complement(10015..12009)
                     /gene="uvrB"
                     /locus_tag="ACG842_001706"
     CDS             complement(10015..12009)
                     /gene="uvrB"
                     /locus_tag="ACG842_001706"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HFQ0248251.1"
                     /translation="MIERDTSRHFELVSKYQPAGDQPEAINQLVDGVVGGKKAQILLG
                     ATGTGKTYTISNLIEKVNKPTLIIAHNKTLAGQLYGEFKEFFPNNAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVNDEIDKLRHSATSSLLERNDVIVIASVSCIFGLGSPFEYQ
                     KQVVSIRQGAELDRNQLIRDLVSIQFERNDIDFQRGRFRVRGDVVEIFPASRDERALR
                     VEFFGDEVERIREVNALTGEVLGETEHVAIFPATHFVTNDEHMEHAVANIKEELEQRL
                     TVLRNENKLLEAQRLEQRTNYDIEMMLEMGYTSGIENYSRHMDGRKEGEPPYTLLDFF
                     PEDFLIVADESHVTMPQIRGMYNGDRARKQMLVDYGFRLPSALDNRPLRLEEFEKHVN
                     QIIYVSATPGPYEHEQTDTVIQQIIRPTGLLDPVIEVRPIMGQIDDLVGEINERVEKD
                     QRVFVTTLTKKMAEDLTDYFKELGIKVKYLHSDIKTLERTEIIRDLRLGEFDVLVGIN
                     LLREGLDVPEVSLVAILDADKEGFLRSERSLVQTIGRAARNEEGKVIMYADKVTDSMR
                     LAMDETSRRRTIQQKYNEEHGIVPKTIIKEIRDLISITKESEDDTKEAVQVSYEEMTK
                     EEKDTLLMKLEKEMKDAAKALDFETAANVRDMILELKASK"
     gene            12268..13770
                     /locus_tag="ACG842_001707"
     CDS             12268..13770
                     /locus_tag="ACG842_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HFQ0248252.1"
                     /translation="MKRKHLFHSFVFLMTGLVTLAFGHVAQADNTTTAESSTSETMQK
                     IEPKKDVYVIASDSAFAPFEFQNAKGEYEGIDVKLMENIAKLQGFNIRMDYRGFSAAL
                     QALESGQADGMIAGMTVTDERKKSYDFSDSYFDSGIQLAVKKGDTSIKSYEDLKGKTV
                     GAKVGTESAEFLSDHESEYGYSIKYFDAADQLYDGLKVGAIDAMMDDYPVIGYAIKQG
                     QELETPIKKESGGKYAFAVKKGQNPELLNMFNEGLKELKRTGEYDKIIKEYVADGSET
                     SSESSTADESTFLGLIQNNYQALLKGLWRTILLTLISFALAIVIGVIFGLFSVAPIRA
                     LRTIASIYVDIIRGIPMMVLAFFIFFGLPGIVGFTIPDFLAGVITLTLNASAYIAEIV
                     RGGINAVPVGQMEASRSLGLSYNRTMQKIILPQAIRIMIPSFVNQFVISLKDTTIISA
                     IGVVELLQTGKIIVARTTQSTYVYLIIAIMYLILITALTKLAKVLEKKVK"
     gene            13772..14509
                     /locus_tag="ACG842_001708"
     CDS             13772..14509
                     /locus_tag="ACG842_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFQ0248253.1"
                     /translation="MAEKILVEHLVKKYGDNTVLNDINVSINEGDVVCIIGPSGSGKS
                     TFLRCLNRLEEPSDGEIIIDGAHLMDKSTDINLVRQHIGMVFQHFNLFPHLSILENII
                     LAPMDLKKESREEAEKKAIKLLQTVGLEDKKDMYPEMLSGGQKQRVAIARALAMNPDI
                     MLFDEPTSALDPEMVGDVLNVMKKLAEQGMTMVIVTHEMGFAKEVANRVMFIDDGNFL
                     EDGTPQQIFENPQNERTKDFLNKVLNI"
     gene            complement(14680..15873)
                     /locus_tag="ACG842_001709"
     CDS             complement(14680..15873)
                     /locus_tag="ACG842_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005336078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFQ0248254.1"
                     /translation="MKEKRRDNKGRILHTGESQRTDRKYLYKYVDAFGNTKYVYAWRL
                     TPTDPTPKGKREKPSLRELEQQIRRDIEDGIDSTGKKMTLCQLYAKQNAQRANVKKST
                     QKQREQLMRLLKEDKLGARSIDTIKPSDAKEWALRMKDKGFSYNTINNHKRSLKASFY
                     IAIQDDYVRKNPFDFKLSEVLENDTKKKVALTEEQEQALLSFIKTDNVYHKHYDDVLI
                     LLKTGLRISELCGLTVADIDFKNEVVIIDHQLLKSKEQGYYIETPKTKSGIRQVPLSK
                     ETIQAFQQVMKKRPKAEPFVIDGRGNFLFVNHKGKPKVAIDYNALFIRMVKKYNKHHK
                     DNPLPHITPHTLRHTFCTRLASKNMNPKDLQYIMGHSNISITMNWYAHASIDTAKSEV
                     QRLIA"
     gene            complement(15952..16158)
                     /locus_tag="ACG842_001710"
     CDS             complement(15952..16158)
                     /locus_tag="ACG842_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HFQ0248255.1"
                     /translation="MSNAQDIPVWEKYTLTIEEASKYFRIGENKLRRLAEENKDAGWL
                     IMNGNRIQIKRRQFEQVIDKLDAI"
     gene            complement(16151..17293)
                     /locus_tag="ACG842_001711"
     CDS             complement(16151..17293)
                     /locus_tag="ACG842_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005336080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HFQ0248256.1"
                     /translation="MNNLEFALEMKNKRLASGLSQGELASLTHVSRYSINRFENGKAN
                     ASKETQNIILRCLNYYICDKPFYLLVDYLSVRYPTTDALEIIRKVLGMKADYFIHYDY
                     GYYGYKEHYAYGEIKVMASDDEHMGVFLELKGAGSRNMEYVLQAQNRDWYSFLNRCLD
                     CDGVIRRFDLAINDMCGLLDIPVLSEKYRNGGADCRCKNYENVQGGKLSGKNRNLAST
                     LYIGSKASTKYFCLYEKQKEQATKKKHTDIINRFEIRLRGTKAVQAVEELLLTYNPHG
                     LVFYLITDFVQFPDYPLWEIFISHDSLPFEMNPVPVNMERTLQWLERQVMPSIVMIEE
                     IDRLTGSNYMKMIDECTHLSEKQEMLVEQMCTDIADVIESEGVFYE"
     gene            17805..18440
                     /locus_tag="ACG842_001712"
     CDS             17805..18440
                     /locus_tag="ACG842_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFQ0248257.1"
                     /translation="MEGYAMKISHLGNNIQTIRKFRGMKQQELADKIGINMQSLSKIE
                     RGLNYPAYETLEKIMEVLDVTPNELLSGEWKYVNQSEKEVCQFLRAEERLNAELKHGH
                     YDNFFDSEEEWLEYELEKLREYITDYINGKSIEASDLYPIKEFIQHLKFQKLLDRYDD
                     LYSMDMFGESIEGHKYKTPYQVVKMINPNSKEDMELLREVLRNNNFDDEDE"
     gene            18594..18671
                     /locus_tag="ACG842_001713"
                     /pseudo
     CDS             18594..18671
                     /locus_tag="ACG842_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345006.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
     gene            18985..19032
                     /locus_tag="ACG842_001714"
                     /pseudo
     CDS             18985..19032
                     /locus_tag="ACG842_001714"
                     /inference="COORDINATES: protein motif:HMM:NF011335.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            19048..20967
                     /gene="tet(M)"
                     /locus_tag="ACG842_001715"
     CDS             19048..20967
                     /gene="tet(M)"
                     /locus_tag="ACG842_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HFQ0248258.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLFPLYH
                     GSAKSNIGIDNLIEVITNKFYSSTHRGPSELCGNVFKIEYTKKRQRLAYIRLYSGVLH
                     LRDSVRVSEKEKIKVTEMYTSINGELCKIDRAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRKKIENPHPLLQTTVEPSKPEQREMLLDALLEISDSDPLLRYYVDSTTHEIILSFLG
                     KVQMEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVS
                     PLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAPQEYLSRAYNDAPKYCANIV
                     DTQLKNNEVILSGEIPARCIQEYRSDLTFFTNGRSVCLTELKGYHVTTGEPVCQPRRP
                     NSRIDKVRYMFNKIT"
BASE COUNT         6175 a         4299 c         3725 g         6785 t
ORIGIN      
        1 tctataaaca tcaccccaaa taaaacagaa aacagtataa gcattataaa aattatcaac
       61 aaccttgatc cttcttttat agattcagga aacagtataa ttttagtact agataaattg
      121 tttacaccga ttagtgttaa aatatcttta ttcttcttgc taacataacc ctttatgcac
      181 aaaagaacag ctatacttaa agtaacaaat aaataacgta ttccaataga aaaatcacta
      241 taaatactct ctagcatagg accgacaatt gaagaagtca ctactgcaaa tgctggacct
      301 atccaagcaa cattagaatg ttctttctct cttttccttt gtaaatcttt gttgagtatt
      361 aatctattta tttcttctgc aggcaagtta attttcataa ccttatgggg gattatccaa
      421 ttaaaaatcg gaaaaaggta gcctccccaa aaagtatagt gatctgtgtc aatcagataa
      481 taacctgatt ttgattccag aataacatat ctcaaagccg aacgaaaatt tttcatgaat
      541 atccataatc tcactgtttc cacacctaat tcctccaatt ccaattaaat ggatttatag
      601 catcaccaat tatgttaact gcctgaccaa cctcattgat aaaattggca ccactatccc
      661 atgcttcaac aatgctatat atctatcaaa caaaaaatgg aaaactaaat actcgcgcac
      721 ataaatttta cattctgttt tttataagac taataataat cattgaaaaa atttcttatc
      781 ggtggcgcat attcccaatt gattaatgtg atcccatatt ctgcaagtat tttacgagct
      841 tcttctaagt catagtgaga aaaatgcata aaacctgcaa tacgatcacg gactgtgagc
      901 tgtggcttgc ttccaccatc attcataaaa tatccagcag agccactact taaagtgatt
      961 gttacatctt cactttctat tattaagaat tcataatgaa atgctttcaa ctctccacta
     1021 ttatttaaaa gttttatact ccaacttcgt ctaggttgag ttgtcacatt ccctctaaaa
     1081 cttcctataa attctgcaat caccgtatct tcaatcaaaa ttctttcctc atctatttca
     1141 tattcaagac taaacggaaa ttctccatat gtaatctctg gcaatggtgg tgctgtagtt
     1201 ctccaaataa atacaataac tgtgaataga aaaaatccaa cgatcgatat aaatgttatg
     1261 agacctgctc gaagtaaaac ccgatataat aaaaatgctg tatttcgctt tgctatatct
     1321 agaaaaataa aaagtactgc aatcataatg atagtaaaag ttgtaaaaaa gcacattttt
     1381 tgccctccct ttataaacat cgattctcaa attctaaacc aaatcttgat agttcaagtt
     1441 caggtataac gtataccaac catacatccc aattactatt tatgaactta tgattatcac
     1501 tgaataaatc actccatccc cttgttaaaa cttctacatt aattacacct ggttgttttc
     1561 gactagctaa tccaatgaac ttatcaacca tccttatcca ccaaccacga gaatcttgac
     1621 cagaagtaag aaaaacagtt ctcaaggttg atcgagcagt tccttccagt ttttaattaa
     1681 tctccagtta tagataaata cttgatccta tctatatcag gacttctata ttaagcaaaa
     1741 gtttatgtat aaaactaaaa tcttcattca gttgtctccc cgctttttat acgattgtat
     1801 taaaaaacaa tagaagcctt tcaacattat gttactccag taaagttgga attatagttt
     1861 gacggaagaa aagcaagtat agcttttaaa aaaaactgat acattcgcat ctcctgcttc
     1921 atgtatcagt tttttctttg gattaaactg cttcttcacg cagtttttcc gcaaattcat
     1981 ttattttgcg aattcggtga ttaattcccg attttgatat tgcacctgag ggaatcattt
     2041 cccctaattc tttcaagctg acctctggat gttgaagccg aagttctgcg atttcctgaa
     2101 gtttttcagg aagggcatgt agaccgacct ttgcttcgat caattcgatg ttttcgattt
     2161 gtttagaagc cgcatcaatc gtcttgttca gatttgctgt ctcacaattg actagacgat
     2221 tgaccgaatt tctcatatcc cgtacgattc gaacatcttc aaatcggagc attgagttgg
     2281 ttgcaccaat caatgttaag aaatcagcaa ttttttctgc tccttttaaa tacgaaatat
     2341 agccattgcg ccgttcaagc gttcgagcat gtagaccgta ataatttaac atatcgcaaa
     2401 tgtcttggtt atgctgttca taaatagaaa atatttccaa atgataacga ctcgtttccg
     2461 gattattgac tgaacctgag gccataaaag caccacgtaa gtaagaacgc attttttgcg
     2521 aatttcccat aatctcgtcc gaaacgtgtc cattgaacat cataccatcc atgatatcaa
     2581 gatccatcaa cacatctttt gtgccttgct tcaaccggac gatatagaca ttgttctttt
     2641 ttaatttcat ttttttacga acgagtagct cacttcgtac ctgataatga tctttcaaaa
     2701 gagaatagat acgtctggca attgcagcat tttctgtttg gacgttcaaa ataaactgct
     2761 ggttcacaat acctaaagag ccgttcattc gcagcaaagc agctaattcc gctttcgcat
     2821 gttctcgatg aacttccaaa ccagttagtt ctttcttcac atctgcagca aatgacatcc
     2881 tattctcctc ctttctccaa caataaattg cttaatattt tgaaccgaaa actaaacgga
     2941 acaattcttc tactactttg tctccatcgt ggaagacacc accatctttc aattctaaaa
     3001 agtcggtgga aatcactcga catccttcat ttctcaatcc gttgaagtcg tgtgcgactt
     3061 gtactagata ctcatcatag atctctgggt ccatataatc ttcaggcact ttttccgtat
     3121 tgaccaatac tgtatcgata aaaggctgat tcaaatgctt gtgtaaaaca cgtatatgat
     3181 cggcatctgt gaaatgttcc gtttcccctt tttgggtcat gatattacag atatagacgg
     3241 tttcagcagt tgtcttgagc attgcttcgc cgatttctgg tatcactaaa tttggaagga
     3301 tactggtgaa taaacttccg ggtcccaaaa cgaccatgtc ggcgtccata attgctgaaa
     3361 cgacttttcg agccgctttg gcttcacttt tatcatttgt gttgcagaca tacacacggt
     3421 caatggtttt acggttgatc gcgattttag attctcctac tgcagtcgtt ccgtctttaa
     3481 aaacagcatg caaggtcaaa ggagtttctg aagaaggata aacatgtcca tctacatgca
     3541 tcatttttgc taataattga atcgcctcat aagtgcttcc ccgcatctcg gaaactgcgg
     3601 caatgatcag attaccgatc gtatgattgg ctaaaaactg atcttccttt ttgaaacggt
     3661 attggaaaat atcttcatag aattgtggca tatccgataa cgccactaag acatttctca
     3721 agtcaccagg aggagccata gcgatcgatt ctctgattgc accgctgctt ccaccatcat
     3781 ctgcaacagt cactactgct gtgatatctg ctccttgatt tcgtaaactt ttcaggataa
     3841 ctggcagacc agtgccaccg ccgaccacta cgatctttgg ttttcttatc cggtaggttt
     3901 tcattttcac gaccggttca ccgtctcttt ccttttatcc ttgtcccgat gtgtcacatt
     3961 gactttatag tttttcccta aagcatttgc caaacgttca gtcaatgcca cagaacggtg
     4021 ttgaccgcct gtacagccga ttgcaactgt caagctgctt tttccttctt taatgtatcc
     4081 aggcaataca gtttccagaa gatccaaaaa tttatggtag aatgattgcg tttcatcaaa
     4141 ggacatcaca taatcataga ctggctgatc ttttccggtc aatggtctta gttccgggat
     4201 ataatgaggg ttgggcaaaa aacgcacatc catcacaata tccgaatcga tcggcaaccc
     4261 gtatttaaaa ccaaatgata caaattcgat ccgaaatcct tggtctccct ttgatttgaa
     4321 tgactcattg attttttcac gtaattgtcg tggtgtcaat gttgtcgtat caatgatcac
     4381 ggaagcttgg gctttcaagt cgtctagtat cgcccgttct ttacgtatcc cttcagtgat
     4441 caaaccgtcc atcgccattg gatgtgctcg tcttgtttct ttgtagcgag ccactagttc
     4501 ttcgtctgaa gcatctaaaa ataagataga agtgtcgatg aagttcgtat tttcgatttc
     4561 gatcagcatg ttttggattt cttcaaagaa ggaacgagaa cgcaaatcca ctcctaaagc
     4621 catttttgtg actttacctg attctttgat caattcccag aattttggaa tcaatcgagg
     4681 cggcatatta tcgatacaaa aatagcccat atcttcaaaa ctttggatag ctactgtttt
     4741 ccctgcacca ctcatacctg ttatgatgac taattgcaaa ttatctgtca tatttacgaa
     4801 cccctctcac attgtgcata ataaaattat atcatcccgg gcgtatcatt accagttttc
     4861 tttttctttt acaaaaagat ttggaccaaa tatcttttag gatatttgcc ccaaatccaa
     4921 tttttttact tttccgatgc tgtatccaat gcctcgcttc tttcagctaa tgcacgttcg
     4981 atcgttcgat ccgctaaaat agattgagca tcgatcactg ggtaatgatt atcttcagca
     5041 aattcatgca tcaaagataa ttctgtgcaa cctaggatga ctttttcaca gtttaggcgc
     5101 tctaccgctt cctctaagat ttcgtaatag agctcttgat tcaaataatc cgattccttg
     5161 atctcatgat agatcaggta attgattttt tcttgaagcg cagtatcagg aatcactgtt
     5221 tcgaaaccaa gatttttgac ttcgcgttca tagattccag ctttcatact tccttctgtt
     5281 cctagaatcg ctactctccc tgtcgtatgc tgacggacaa gttcatttgc tgcttctctt
     5341 ggcatatgca agataggaat atccgttgcc gcttgtaact cttcaaaaaa gtagtgtgcg
     5401 gtgttacaag tcaacacaat gaagtttggt cgcaataaat tttgtttctc aatatcatcc
     5461 agcagaaacg gcattggatt ttcttctgat cgatctaaaa tataagctgt acgatcagga
     5521 actgttgcat gattgaataa gacataattc aaatattctt gatcttttgt agctttcgtt
     5581 cggtggttga tcaagcgaac aaaactttct gtcgccatcg tgcccattcc gcctaaaata
     5641 ctgaaaaaat tctccatgaa ctagccttct ttcacatgaa aatacttttt gaatcttgga
     5701 atatagttat ggaacatata tttcatcaac aaccagcgct tgattgatcg gtctttttca
     5761 taaaatacag tagtaccgta acgattttct ttgatcattt gctcagctag ttttttgtct
     5821 tcattttccc gagcatattc taaaaagatt tttttgggca cacccatcca taatcttgct
     5881 ttatctgatt gattcgttcc ataggtcgtt tcaacaaagg gtttgttgaa cactcgatct
     5941 tctgtgacaa aacgagccag attcaagccg ttcaatgtca caaagaaact gctacgacct
     6001 tgtctcaaat tgatttcaaa taatttatat tctccatctc taggatcata cttcatatcg
     6061 aaattggcaa agcctgtata ttcgattttt tccaaaaatg ccttaatggt ttgatagatt
     6121 ttttcgttat aatctggcat gatcacaacg taattaccaa tcgatgctgg tgtaggatct
     6181 tctaatagtg gatgaccaag gcacatcatc cgaacttgat gatcttcatc tacatacgca
     6241 tttagcacac gcatattact atcatcaccc gggataaaat cttgtacaat catttcactt
     6301 ttataaccgg cttcgtatat ccggcctaaa attaaatcga actcttctct agtctctaaa
     6361 ataaaagctt tttttcttcc ttcaaattgg acagaaagat actccacact gtttgcaggt
     6421 ttcaaagcaa ctgggaattc aaaaggcaat tcctgttcca aatgtccatt tactaacatt
     6481 tcttctctga cgatcaatgt tttaggatac ggaagatcat attcttcaca aacttcataa
     6541 aagcttactt tattgatcag cctttcaaat aaagaataat caatatatgg acagataaaa
     6601 tattctgaca gctcttgttt atgttgggag atcaattctg catatccatc accacaagca
     6661 atcaaaagat attttttact cttatcagaa tatctttctt ttgccaaacg aagcatcgtc
     6721 tcgataaata cagggtcttt atcaaatcct ggaataactt ctacattgac aattttactg
     6781 tagcgggtag gggctaactg atccgaagca tatgcttggc agattttgcc gtatgcttca
     6841 ttaaaagaac gtgccatacc ataaacgttc atgtcgcttc ctaataatat agggataaat
     6901 tcttcatttt caatactgtt catcattatc tctcacttac tcataatctc ttgtttttct
     6961 tttccaacta cacaaggaat atcataccat aaagaaaaaa gcaatcaaag gaaagtcctc
     7021 cgattgctca attctttttt gaacggattg aacttatagg aagtttttat ctataccaat
     7081 cagtgtagaa agaactacca tgtaatgatt ctagaacact gatattaata aaacaagcaa
     7141 aaattcattt ttttatgttt ttttaactat ctcagctatc tatcctcttt ataccattat
     7201 ttttttcaaa tagtgacctg tataactttc ttttacgtta atgatttctt ctggtgttcc
     7261 agtcgcaagg atcgttcctc caccttctcc accttctggt cctaaatcaa tgatataatc
     7321 tgctgttttg attacatcta gattgtgttc aatcactaaa actgtgttac cagcatctac
     7381 taatctttgt aatacatgca acaagcgggc gatgtcatcg ctatgaagtc ctgtcgttgg
     7441 ttcatctagt atatagaaat tctttccatt agagattttg tgcaattcac tggcaagttt
     7501 catccgttgt gcctcaccac cggacaatgt cgttgctggt tgccccatcg tcacataacc
     7561 taagccaaca tcaacaatcg tttgcagttt gcgatgaatc tttggaatgt gcttgaagaa
     7621 ttctacagca tcctctactg tcatttccaa aatatcagca atgctttttc ctttgtaatg
     7681 cacttctaat gtttcagaat tatatcgttt accatgacat acttcacaag gaacatagac
     7741 atcaggcaaa aagtgcattt cgatcttaat aattccatcc ccgcgacaag cttcacaacg
     7801 accgcctttt acgttgaaac taaaacgccc tttcttataa ccccgcattt ttgcctcgtt
     7861 cgtttgagca aataacccac ggatatcatc aaatacactt gtataagtcg ctggattact
     7921 tctcggcgtc cggccgattg ggctttgatc gatatcgatg atcttttcga tactttcgta
     7981 gccggaaatt gtcttgaatt tacctggctt agcagaattc ttatttaatt tttgcgctaa
     8041 cgatttcttt aagatactat tgaccaatgt actctttcca gaaccagaca cacctgtgac
     8101 tgctacaaat tctcctaatg gaaattcaac actaatattt ttcagattgt tttctgaagc
     8161 accagtgatc ttgattgctt tcccattacc tttacggcgt tcttttggta cagggattga
     8221 ttttttccct gaaagatatt tccctgtcaa agaattttca tcattagcta cttcttcagg
     8281 agtgcctgca gccacgattt gtccgccttg ttctccggcc ccaggtccga cgtcaatcaa
     8341 ataatctgct gcccacatcg tatcttcatc atgttcgact acaacgaggg tgttgccaag
     8401 atcacgcatt ttctttaatg agccaatcag tcgatcgttg tcgcgttgat gcaatccgat
     8461 cgatggttca tctaaaatat acaaaactcc agaaagattc gatccaattt gtgtagccaa
     8521 tctgattcgc tgcgcttctc ccccagataa tgttccggaa gcgcgactca aagttagata
     8581 gtctagtccc acattttcca agaatgaaag tctgtcattt acttctttta aaatcggttt
     8641 ggcaattact ttctcttgtt cagataacgt taagtttgag aagaaatcta ctgctttttg
     8701 aatagacaac tcatttgttt gcccgatatt cgttccattg atttgaacag atagagcttg
     8761 gggattcaat cgataacctt tacatgtctg acaagttaat tctgtcatat aaagacgcat
     8821 ttgctcacgc gtgaaatcac tgttcgtatc atgataacga cgctcgatat tcttcaatac
     8881 cccttcgaaa gtcgtttcta catcgcgaac gccaccaaaa tcattttcat aatggaaatg
     8941 gaaaagatca tctgtcccat tcaagatgat ctcctgatgc tcttccggta attcttcgaa
     9001 tggtgtatct aaatcgatac cgaaactttc acaagcctgc gctaacattt gaggataata
     9061 ctgagagctg attggattcc atggaacgat agcaccttga ctaagtgtct tcgttctatc
     9121 tggaatcact aaatcttgat cgacttctaa tttgacacct aaaccatcac agtctggaca
     9181 agctccaaaa ggcgcattga atgagaacag tcttggttct agttcgccaa cagtaaatcc
     9241 gcaataagga caggcatagt gttcgctaaa caacatttct tcttcaccga tcacgtcaac
     9301 cagagcatag ccgtctgcta atcgcagggc cgcctctaaa gaatcaaata ggcgagaacg
     9361 aatcccttct ttcacaatga tccggtcgat gacgatcgcg atatcatgtt ttttgttttt
     9421 ctccagctca ggcacttcac ttaaatcata gatctcacca tcgactcgaa cgcggacgta
     9481 tccctctttt tggattcgtt cgaatatttt tttatgttgt ccttttttct ttaccacgat
     9541 cggtgccaac acttggatct ttgtgcgctc aggcaaagcc attacttgat ctgccatttg
     9601 atctactgat tgactggtaa tctcgatatg atcgttggga caaatcggat gtcctacacg
     9661 tgcaaacaac aagcgtaaat agtcattgat ttccgtcaca gtacctactg tcgaccgagg
     9721 atttttactt gttgttttct gatcaataga aatcgccgga ctcaatccgt cgatgctatc
     9781 tacgtccggc ttatccattt gtcctaaaaa ctgacgagca taagcagaaa ggctctctac
     9841 gtatctccgc tgcccttcag cgtacagcgt atcaaatgct agggaacttt ttccagaacc
     9901 ggacaaacct gtaacgacca cgaatttatc tcgtggaatc gttacatcta tatttttcaa
     9961 gttatgggca cgtgcaccat gaatcgtaat tttatcgtta gccatgttgt cctcctactt
    10021 tgaagctttt aattctaaaa tcatatctcg cacattcgcg gctgtttcaa aatccaaagc
    10081 ttttgccgca tctttcattt ctttttctaa tttcatcaat aacgtgtctt tttcttcctt
    10141 cgtcatttct tcgtacgaga cttgtaccgc ttcttttgta tcatcttccg attctttcgt
    10201 aatagaaatc aaatctcgga tttctttaat gatcgttttt ggaacgatcc catgttcttc
    10261 attgtacttc tgctgaatcg tacgacgtct tgacgtttca tccatcgcta gacgcataga
    10321 atctgttact ttgtctgcgt acatgattac tttaccttct tcattccgag cagctcgtcc
    10381 aatcgtttgg actaaggaac gttcactacg taaaaagcct tctttatccg catccaagat
    10441 agcaacaagg gaaacttcag gtacgtctaa tccttcacga agcaaattga ttccaactaa
    10501 cacatcaaat tcacctaatc gaagatctcg aatgatttct gttcgttcca gcgttttgat
    10561 atcactatgc aaatatttga ctttgatccc taattctttg aaataatctg tgaggtcttc
    10621 tgccattttt ttcgtcaatg tcgttacaaa cacccgctgg tctttttcga ctcgttcatt
    10681 tatttcgcct actagatcat cgatttgtcc catgattgga cgaacttcga tcactgggtc
    10741 aagcagccct gtcggacgga tgatttgttg gataacggta tctgtttgtt catgttcata
    10801 tggtcctggc gtcgcagaga cataaatgat ctgattgaca tgtttttcaa actcttctaa
    10861 ccgaagtggc cgattgtcta aagcagatgg caaacggaaa ccgtaatcta ctagcatctg
    10921 ttttctcgct cggtcaccat tatacattcc tctgatttga ggcatggtta cgtgcgattc
    10981 gtccgccacg atcaaaaaat cttcaggaaa gaaatccaat agcgtataag gcggttcacc
    11041 ttctttacgt ccgtccatat gccgagaata attttcaatt cccgacgtgt agcccatttc
    11101 taacatcatt tcaatatcgt aattggttcg ctgttctaaa cgttgagctt ccagaagttt
    11161 gttttcattt cgcaagacag ttaaacgctg ttctaattcc tctttgatat tcgctacggc
    11221 atgctccata tgttcatcat tcgtcacaaa gtgagtcgcc gggaagattg ctacgtgttc
    11281 cgtttctcct aaaacttcac ctgtcagtgc attgacttct cggatacgtt cgacctcatc
    11341 tccaaaaaac tccacacgta aggcacgttc atctcttgaa gccggaaaaa tctccacgac
    11401 atcaccgcgc actcgaaaac gaccgcgctg aaaatctata tcatttcttt cgaattggat
    11461 actgactaag tcacgaatca actgattgcg atctagttct gctccctgtc gaatcgaaac
    11521 tacttgtttt tggtactcaa aaggcgagcc taatccaaaa atacatgaaa cggatgcaat
    11581 gacgatcaca tcatttcttt ctaataatga actagtagcc gaatgtcgta atttgtcgat
    11641 ctcatcattt acacttgaat ctttttcgat atacgtatcg cttgaaggca cataagcttc
    11701 aggctgataa taatcataat aagaaacaaa atattcaaca gcattgttag ggaaaaattc
    11761 cttgaattcc ccgtacagct gaccagccaa tgttttgtta tgggcaataa tcagtgttgg
    11821 tttattcact ttctcaataa gattggaaat cgtatatgtt ttccctgttc cagtcgcacc
    11881 taataagatt tgtgcttttt tcccgccgac tacgccatcc actaattggt taatcgcttc
    11941 aggctgatca ccagccggtt ggtatttcga gacaagctca aagtgtcggg aggtatctct
    12001 ctcaatcatt cttcgtcctc cttttcattc attccttttc attttacgac caataaaaag
    12061 aaaaggaaag caaaggcatt ccttactaaa aaattatacc acaccgaaca tactttcgcc
    12121 acttttttat cttattatac atgttatgtt ttataacata aaatgttaga aactcgcatt
    12181 tttttcattt tttcgagctt acagtcttgt ctgattcagc acttttcgcc tataataata
    12241 aaatattgga aatttaggag gttgattatg aaaagaaagc atttatttca ctcatttgtt
    12301 ttcttaatga caggacttgt gacacttgcg tttggtcatg ttgcacaagc tgacaacact
    12361 acaacggcag agtcaagcac aagcgagaca atgcaaaaaa tagagcccaa aaaagacgta
    12421 tacgtaattg ccagtgactc tgcttttgct ccatttgaat ttcaaaacgc aaagggcgag
    12481 tacgaaggca tcgatgtaaa attgatggaa aacatcgcca aactgcaagg atttaacatc
    12541 cgaatggact accgtggttt ctctgctgct ttgcaagctt tggaatcagg acaagcagac
    12601 ggaatgattg ccggaatgac tgtaacagat gaacgaaaga aaagctatga tttttctgat
    12661 tcttactttg atagcgggat ccaattagct gtcaaaaaag gagatacttc gatcaagtct
    12721 tatgaagatt tgaaaggaaa aacagtcgga gcaaaagttg gtaccgaaag tgctgaattt
    12781 ttaagcgacc atgaatctga atatggctat tctatcaaat attttgatgc tgctgatcaa
    12841 ttgtacgacg ggttaaaagt tggcgcaatc gatgcaatga tggatgatta tccggtaatc
    12901 ggttatgcga tcaaacaagg acaagaattg gaaacaccga tcaaaaaaga atctggcgga
    12961 aaatatgcat ttgccgttaa aaaaggacaa aatcctgagc ttcttaatat gttcaatgaa
    13021 ggattgaaag aattaaaaag aacaggcgaa tacgataaga ttattaaaga atatgtagcg
    13081 gatggttcag aaacttccag tgagtcttcc actgctgatg aatcaacatt tcttggactt
    13141 atccaaaaca actatcaagc attgttgaaa ggactttgga gaacaattct cctaactttg
    13201 atctcctttg ctttggctat agtaatcggt gtgatttttg gattattcag tgtagcacca
    13261 atacgcgctc ttcgcacaat tgctagtatt tatgtcgata tcatccgagg aattccaatg
    13321 atggttcttg cgttctttat tttctttggg ttgcctggta tcgttggatt tacgattcct
    13381 gacttccttg ctggtgttat caccttgact cttaacgcaa gtgcttatat cgcagagatt
    13441 gttcgaggcg ggatcaacgc cgttcctgtc ggacaaatgg aagcttccag aagtttaggt
    13501 ctttcttata atcggacaat gcaaaaaatc attttgccgc aagcgatacg catcatgatc
    13561 ccatcattcg tcaatcagtt cgttatctca ttaaaagaca caacgatcat ttccgcaatc
    13621 ggtgtagtcg aattgctgca aactggtaag atcattgttg cacgtacaac acaaagtacg
    13681 tatgtttact tgatcattgc gatcatgtac ttgattttga tcacagcatt gacaaaatta
    13741 gcaaaagttt tagaaaagaa ggtgaagtaa gatggcagaa aaaatcttag ttgagcattt
    13801 agtcaaaaaa tacggcgata acacagtatt aaacgacatc aatgtatcca tcaacgaagg
    13861 agatgtcgtc tgtatcattg gcccttcagg ttcaggaaaa agtacattct tgcgttgctt
    13921 gaatcgttta gaagaacctt ctgacggtga aattattatt gacggcgccc acttgatgga
    13981 taaaagcaca gatatcaact tggtgagaca acatatcgga atggtattcc aacactttaa
    14041 cctattccct catctttcta ttttggaaaa catcatttta gcaccgatgg acctgaagaa
    14101 agaatctaga gaagaagcag agaaaaaagc aatcaaatta ctgcagacag ttggtctaga
    14161 agataaaaaa gatatgtatc ctgaaatgct ttctggtgga caaaaacagc gtgtggctat
    14221 tgctcgtgct ctagcgatga accctgacat catgcttttt gatgagccta cttctgcact
    14281 cgatcctgaa atggtcggtg atgtcttgaa cgtcatgaaa aagttagcag aacaaggtat
    14341 gacgatggtt atcgttacgc acgaaatggg gttcgctaaa gaagtggcca atcgcgtcat
    14401 gttcattgac gacggaaact ttttagaaga tggaacgcct caacaaatct ttgaaaatcc
    14461 gcaaaatgaa cggacaaaag atttcttgaa taaagtatta aatatttaaa ataaattata
    14521 tatgtctttt tgactatctt tttatatctt cttaaatgtc cgcaaagcct tattttatag
    14581 gctctacggg cattttgctt ttgtggtaaa cctcacatat ctaggtctat cttcttatat
    14641 tttcactatc aagcgtggtt aaaatcgtgg taaatacttc tatgcaatta gacgctgaac
    14701 ctctgatttt gcggtatcta tggacgcatg agcataccag ttcattgtga tactgatatt
    14761 tgaatgcccc atgatatact gtaaatcttt tgggttcatg ttcttgcttg ccagtcttgt
    14821 gcagaatgta tggcgtagcg tatgcggtgt gatatgtggc aatggattgt ccttgtggtg
    14881 cttgttgtat ttctttacca tacggataaa taaggcgtta taatcaatcg caactttggg
    14941 cttgccctta tgattgacaa ataggaagtt gccccgtccg tctatcacaa atggttctgc
    15001 ctttggacgt tttttcataa cctgttgaaa tgcctgtatc gtttctttgc ttaatggtac
    15061 ttgccttatt ccgctttttg ttttaggcgt ttcaatataa tagccctgtt ccttgctctt
    15121 tagtaactgg tggtcgataa tcacaacttc attcttgaaa tcaatgtcgg ctactgtcag
    15181 tccgcacagt tccgaaatac gaagtcctgt ctttaacagt atcagcacat catcataatg
    15241 cttgtgatac acattgtccg tcttgatgaa tgagagtaag gcttgttcct gttcctctgt
    15301 caatgcgact ttctttttgg tatcattttc taggacttca ctcaacttga aatcaaaggg
    15361 atttttcctc acataatcat cttgaatggc tatgtagaat gacgctttta acgagcgttt
    15421 atggttgtta atggtattgt aggaaaagcc tttgtctttc atgcgtaacg cccattcctt
    15481 agcgtcagag ggtttgattg tatcaatgct cctagcacct aacttgtcct ctttcaataa
    15541 ccgcatgagt tgttcccgtt gtttctgtgt gcttttcttc acatttgccc tctgtgcgtt
    15601 ctgtttggca taaagttggc aaagcgtcat tttcttgcca gtgctgtcga taccgtcctc
    15661 aatatcccgt cttatctgct gttccagttc tcgaagtgag ggtttttccc gttttccctt
    15721 tggtgtcggg tctgtgggtg tcaatctcca agcatacaca tattttgtgt ttccaaatgc
    15781 gtctacatat ttatataagt attttctgtc tgttcgttgg ctctctccag tatgcaggat
    15841 acgacctttg ttatcccgtc ttttttcttt catggtgtct gctcctttcc ttgttggaaa
    15901 gagccttgat atgacttgtg ttcatcataa cacatacaag gctcatttgc attagatagc
    15961 gtccaattta tcaatgacct gttcaaactg tcggcgttta atctgtatgc ggttgccatt
    16021 cataatgagc cagccagcgt ccttgttttc ctctgccaat cgtcttaact tgttttctcc
    16081 gatacggaaa tactttgacg cttcttcaat ggtaagggtg tatttttccc atacgggaat
    16141 atcctgtgca ttactcataa aacaccccct cactttcgat tacgtctgct atgtccgtac
    16201 acatctgttc cacaagcatt tcctgctttt cagagaggtg ggtacattca tcaatcattt
    16261 tcatgtagtt tgagcctgtc agcctgtcga tttcctctat catcacgatt gacggcatga
    16321 cctgtctttc cagccattgc agagtgcgtt ccatattgac aggtacgggg ttcatttcaa
    16381 aaggtaaact gtcatgggaa atgaatatct cccacagcgg atagtcggga aattgcacaa
    16441 aatcagtaat aaggtaaaac accagcccat gagggttata tgtcaataac aattcctcaa
    16501 ctgcctgtac tgcttttgta ccacgcagac gaatttcaaa acgattgata atatccgtat
    16561 gtttcttttt ggttgcctgt tccttttgtt tttcatacag gcagaagtat tttgtgcttg
    16621 cctttgaacc gatataaagg gtgcttgcta aattacggtt ttttccgctt agttttccac
    16681 cctgcacatt ttcataattt ttacagcgac aatccgcacc gccatttctg tatttttcag
    16741 acaaaacggg aatatccaac agaccacaca tatcattgat tgccaagtca aaacgtcgga
    16801 taacaccgtc acagtcaaga cagcggttta aaaatgaata ccaatctctg ttctgtgcct
    16861 gcaagacata ttccatgttg cgtgagcctg ccccttttaa ttccaagaat acgcccatgt
    16921 gttcgtcatc agaagccatg accttgattt ctccgtaggc gtaatgctct ttatagccat
    16981 aatacccata gtcataatgg ataaaataat ctgctttcat gccaagcacc tttcggataa
    17041 tttccaatgc gtcagtcgtt ggataacgga cagacagata atcaacgagc agataaaaag
    17101 gcttatcgca gatataataa ttcagacaac gcaggataat attttgtgtt tccttgctgg
    17161 cgttggcttt tccgttctca aagcggttga tactatatct tgatacatgg gtaagacttg
    17221 caagttctcc ttgtgaaaga ccgcttgcaa gacgtttgtt cttcatttca agtgcaaatt
    17281 ctaaattgtt cattgtattc ctccaatccc acaatagcct tgaaatacct gtaaacctct
    17341 attcaaaaag tgtgatatgc actatcttct taaaacttct cattactact cacaactttg
    17401 ggattgtatg gtttttgact tgtcttttgt gaaatagtgc ccccctgtta gataacgggg
    17461 gcacagacaa ggctcgcagg ctcgccagcc gacaaagcat ttcttcccct tgacacaagg
    17521 tgtcatggcg gtaagcctga gaggcttccg ccaccgtgtc agaaatactt tgtcagcaag
    17581 ttctgttttg ccttttctaa caagttccat ttcaaccgat attcatatct acggatttaa
    17641 tcagataggt atttcttttt gaatgttttt atatttcatt ctattgtgct tattcgatta
    17701 caagaacata gtaattctta tatgcttatt tgtcaatatc tttttttgcc tttacaaatc
    17761 atattaagca tgatagactt ataatagcga agatataacg agatatggag ggttatgcaa
    17821 tgaaaataag ccatttagga aataatatac agaccataag aaaatttagg ggaatgaaac
    17881 aacaggaact tgcggacaaa atcggtatca atatgcagag cctttccaag attgaaagag
    17941 ggttgaacta tcctgcctat gaaacgctgg aaaagataat ggaagtgctg gacgttacgc
    18001 ccaatgaatt gttgtcggga gaatggaagt atgtcaatca atctgaaaag gaagtctgtc
    18061 agtttttaag agccgaagaa cgtctaaatg cagaactgaa acatggacac tatgataact
    18121 tctttgacag cgaggaagaa tggctggaat atgagttaga aaagttacgg gaatatatta
    18181 cagactatat caacgggaaa agcattgaag cgtccgacct ttacccaatc aaagaattta
    18241 tccagcattt gaagtttcaa aaattattag accgctatga tgatttatac agtatggata
    18301 tgtttgggga aagcatagag ggacataagt ataagacacc ttatcaagtc gtgaaaatga
    18361 taaacccgaa ctcaaaagaa gatatggaac tgttacgaga agtattaagg aataacaatt
    18421 ttgatgatga ggacgagtaa cttttttcct cttgcgagaa gatacagcaa acaaaaagcc
    18481 cgatcaagag tgcaaagcac caccaatggt ggcaaagctc ttgacagggc tttttctttt
    18541 ctgttgtggg caatcaagag gaagaaaggg ataaaatctg atttgtggta aatcaggcaa
    18601 aggcaacgca ggtatctcaa tttgatttgg tacttgaaaa gaacgggagt aattggaaga
    18661 ttatagaata acaaatattg gtacattatt acagctattt tgtaatcacg tactctcttt
    18721 gataaaaaat tggagattcc tttacaaata tgctcttacg tgctattatt taagtatcta
    18781 tttaaaagga gttaataaat atgcggcaag gtattcttaa ataaactgtc aatttgatag
    18841 tgggaacaaa taattggatg tcctttttta ggagggctta gttttttgta cccagtttaa
    18901 gaataccttt atcatgtgat tctaaagtat ccggagaata tctgtatgct ttgtatgcct
    18961 atggttatgc ataaaaatcc cagtgataaa agtatttatc actgggattt ttatgccctt
    19021 ttgggttttt gaatggagga aaatcacatg aaaattatta atattggagt tttagctcat
    19081 gttgatgcag gaaaaactac cttaacagaa agcttattat ataacagtgg agcgattaca
    19141 gaattaggaa gcgtggacaa aggtacaacg aggacggata atacgctttt agaacgtcag
    19201 agaggaatta caattcagac aggaataacc tcttttcagt gggaaaatac gaaggtgaac
    19261 atcatagaca cgccaggaca tatggatttc ttagcagaag tatatcgttc attatcagtt
    19321 ttagatgggg caattctact gatttctgca aaagatggcg tacaagcaca aactcgtata
    19381 ttatttcatg cacttaggaa aatggggatt cccacaatct tttttatcaa taagattgac
    19441 caaaatggaa ttgatttatc aacggtttat caggatatta aagagaaact ttctgccgaa
    19501 attgtaatca aacagaaggt agaactgtat cctaatatgt gtgtgacgaa ctttaccgaa
    19561 tctgaacaat gggatacggt aatagaggga aacgatgacc ttttagagaa atatatgtcc
    19621 ggtaaatcat tagaagcatt ggaactcgaa caagaggaaa gcataagatt tcagaattgt
    19681 tctctgttcc ctctttatca tggaagtgca aaaagtaata tagggattga taaccttata
    19741 gaagttatta ctaataaatt ttattcatca acacatcgag gtccgtctga actttgcgga
    19801 aatgttttca aaattgaata tacaaaaaaa agacaacgtc ttgcatatat acgcctttat
    19861 agtggagtac tacatttacg agattcggtt agagtatcag aaaaagaaaa aataaaagtt
    19921 acagaaatgt atacttcaat aaatggtgaa ttatgtaaga ttgatagagc ttattctgga
    19981 gaaattgtta ttttgcaaaa tgagtttttg aagttaaata gtgttcttgg agatacaaaa
    20041 ctattgccac agagaaaaaa gattgaaaat ccgcaccctc tactacaaac aactgttgaa
    20101 ccgagtaaac ctgaacagag agaaatgttg cttgatgccc ttttggaaat ctcagatagt
    20161 gatccgcttc tacgatatta cgtggattct acgacacatg aaattatact ttctttctta
    20221 gggaaagtac aaatggaagt gattagtgca ctgttgcaag aaaagtatca tgtggagata
    20281 gaactaaaag agcctacagt catttatatg gagagaccgt taaaaaatgc agaatatacc
    20341 attcacatcg aagtgccgcc aaatcctttc tgggcttcca ttggtttatc tgtatcaccg
    20401 cttccgttgg gaagtggaat gcagtatgag agctcggttt ctcttggata cttaaatcaa
    20461 tcgtttcaaa atgcagttat ggaggggata cgctatggct gtgaacaagg attgtatggt
    20521 tggaatgtga cggactgtaa aatctgtttt aagtatggct tatactatag ccctgttagt
    20581 accccagcag attttcggat gcttgctcct attgtattgg aacaagtctt aaaaaaagct
    20641 ggaacagaat tgttagagcc atatcttagt tttaaaattt atgcgccaca ggaatatctt
    20701 tcacgagcat acaacgatgc tcctaaatat tgtgcgaaca tcgtagacac tcaattgaaa
    20761 aataatgagg tcattcttag tggagaaatc cctgctcggt gtattcaaga atatcgtagt
    20821 gatttaactt tctttacaaa tggacgtagt gtttgtttaa cagagttaaa agggtaccat
    20881 gttactaccg gtgaacctgt ttgccagccc cgtcgtccaa atagtcggat agataaagta
    20941 cgatatatgt tcaataaaat aacttagtgt attttatgtt gtta
//
LOCUS       DAZKLM010000053        20465 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3764
            SAMEA115706913-rid22582563.denovo.054, whole genome shotgun
            sequence.
ACCESSION   DAZKLM010000053 DAZKLM010000000
VERSION     DAZKLM010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706913
            Sequence Read Archive: ERR13196647
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20465)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20465)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287823.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287823.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,004
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,849
            CDSs (with protein)               :: 2,849
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 41 of 96
            Pseudo Genes (incomplete)         :: 52 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 15 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20465
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706913-rid22582563.denovo.054"
                     /isolate="VRE3764"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            291..1430
                     /locus_tag="ACG842_001716"
     CDS             291..1430
                     /locus_tag="ACG842_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgE/DapE family deacylase"
                     /protein_id="HFQ0248259.1"
                     /translation="MKKEEKIAILQEIIRIKSVNGNEGEVAAYLNKLLARHDITGEIV
                     SYRDGRDNLIARYQKGQSGKVLGLSGHMDVVAAGDESSWTYAPFAAEIHGNRLYGRGA
                     TDMKSGLAAMVIAMIELKESGKPFNGTVKLLATVGEEVGELGGEQLTKAGYVDDLDAL
                     IIGEPTNYSLMYTHMGSINYTVTSHGKEAHSSMPDQGYNAINHLNEFITKANAEMNHL
                     AETIENPVLGKTIHNVTLISGGNQVNSIPSHAQLQGNIRSIPEYPNDKIIALLQSIVN
                     ELNQETDYHLELMIDYNKIPVKADPDSPLIHSIQQQFSQPLPLVGAAATTDAAEFTKA
                     NHSFDFVVFGPGVVTLPHQVDEYVEIDNYLDMIEKYQGIILSYLA"
     gene            complement(1497..2072)
                     /locus_tag="ACG842_001717"
     CDS             complement(1497..2072)
                     /locus_tag="ACG842_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFQ0248260.1"
                     /translation="MNKNKMATRVLTVMALCIGINYIGGTIALWLRLPIYLDSIGTIF
                     AGALLGPVPGMLTGLSSGILSGVTTDIFSLYYSPIQILTGLLAGLILPRYLGKDKNSL
                     KLLLLALILSVPGTIVSSIITVQLFGGITSSGSSMIVQLLHGLGLSQTTSVILVQVGT
                     DYLDRLLSIVVVSLVITMLPQRLIYSKRKIG"
     gene            complement(2077..3051)
                     /locus_tag="ACG842_001718"
     CDS             complement(2077..3051)
                     /locus_tag="ACG842_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HFQ0248261.1"
                     /translation="MRKVIIDCDPGIDDTLALSLAVKSPDIEVVAITVVCGNVPVDIG
                     TQNVLKCLERCDRLDIPVYQGMEKPLKQPFISAQDTHGLDGLGDTNFPMVLAKQAESL
                     HAVDFLTDYFKEKTDTSVIALGPLTNIASALKNSPSVGKHMDRFVSMGGTYKSHGNCS
                     PVAEYNYWCDPEAASYVFEKLKQPIEMVGLDVTREIVLTPTILEYCCQMNPEEGEYLK
                     AITRFYFDFHWKQERILGCVINDPLAVAYFIDESICEGFKSFTAVETQGISRGQTLVD
                     RYEFWQKPANSKIMTKVDTRLFFRKFLTVLLNAQEETIMKDLERLKMG"
     regulatory      complement(3075..3119)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            3334..4302
                     /locus_tag="ACG842_001719"
     CDS             3334..4302
                     /locus_tag="ACG842_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFQ0248262.1"
                     /translation="MVIFYFILILIFIGISYHFLSYFYAKKLVRAGVQRGYDWYEETG
                     HRLMDPAAVTSVEKKRQKLEEECESFWEQGIPKTIKSYDGLKLAGQMFLQPTQQNKWV
                     ICVHDYRSTGKRDMSHIGKRYAEKGFNVLIPDLRAHGESEGEIIGMGWLDRLDLIAWI
                     QLILDEQPDASIILHGSSMGASTIMMASGEKLPSAVKGFILDSGYVSVYAEFRYMLSK
                     ITVFPKKMIMRYANHYAQKYAGYSLKQASATRQLGSNHLPLLVIHGERDHFVPTEAAY
                     TIQNATAGDKALLLVPEAEHLEASMKDPKTYWTVIFSFIEQRITMD"
     gene            4415..4693
                     /locus_tag="ACG842_001720"
     CDS             4415..4693
                     /locus_tag="ACG842_001720"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0248263.1"
                     /translation="MEIKEEKNRLALFNDEQQEIGEMTWSDAGPDIMIIDHTFVDPAY
                     RGQKLAEKLVYTGVELARREGKKIIPSCPYAKKEFEKKPEYHDVLRQS"
     gene            4811..5047
                     /gene="secG"
                     /locus_tag="ACG842_001721"
     CDS             4811..5047
                     /gene="secG"
                     /locus_tag="ACG842_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015136 - sialic acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015739 - sialic acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HFQ0248264.1"
                     /translation="MYNLILGIVIVLSIVMVIAIMMQPSKQNSAASAFTGGADQLFGK
                     QKARGFEAVMQRSTAVMGAVWMILLFVLAFLSSK"
     gene            5150..5905
                     /locus_tag="ACG842_001722"
     CDS             5150..5905
                     /locus_tag="ACG842_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFQ0248265.1"
                     /translation="MKRVKQLPKPLYAKHGKRAVLLLHAYSGSPNDVRMLARFLEKAD
                     YTVYAPLFKGHGTMEPYDILQEKAESWWADTKKAIHFLQSEQFSDIAVLGLSMGGIFA
                     VRALEEESVIGGGFFCSPLSPVETNVPENFEKYVRQVLKIAGKSEKEINEKAVSYRSL
                     AEHQLMDIQDQAAIAESRLSDIQQPIFLAQAGKDEMIDPNGVFETARKLSRQRVTLQW
                     YPESGHVITVGTARRELEKDVLEFLEKLPWNEE"
     gene            5908..8265
                     /gene="rnr"
                     /locus_tag="ACG842_001723"
     CDS             5908..8265
                     /gene="rnr"
                     /locus_tag="ACG842_001723"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HFQ0248266.1"
                     /translation="MTKETIKEKILFFMESSRKRSFSMEEIAEGLGFQKSDDFKLLVQ
                     TIAQMEREQSIVFNKKGKVKLPLKPILVEGTFRANERGFGFVTIDPEEDDIYIPKEAT
                     GFAMDGDTVAIDIMKRANTDMDRGAEGKVVEIRDRATTQLAGEFVAYSDEEIQETDLY
                     GVVIPKDRKLNQFKVYAAAEGVRPVDGSIVLIELTHYPEKGYATSLEGIIKQVIGHKN
                     DPGMDILSIVVANGIPTKFPDDVLSEADEVPDQISEKDIVGRRDLRDQLIVTIDGEDA
                     KDLDDAVTVKKLENGNYFLGVHIADVSYYVTQGSQLDREAYERGTSVYLTDRVIPMIP
                     QRLSNGICSLNPHVPRLTMSCEMEIDPNGQVVSHEIFPSVIQTTERMTYTAVNQILED
                     QDEETMQRYAQLVPMFQEMGELHRILEMMRIKRGAISFEDREAKILVDAEGHPNDIQL
                     RSRGIGERLIESFMLAANETVAEHYNKLNLPFIYRIHEQPKEEKMQRFFDFASALGIL
                     VRGTKGTITPKDLQHVIEGVEDKPEAAVINTMLLRSMQQARYSEDNFGHYGLAADYYT
                     HFTSPIRRYPDLIVHRLIRSYSEDKSESNQSSWAAELPEIADHSSKMERRAVETEREV
                     DSMKKAEFMMDKVGEEFDGIISSVVKFGLFIELPNTVEGLIHINELKQDYFHFVENHL
                     ALVGERTGLTFKIGQKVRVKVVKADPDERAVDFELVSAEEVTALERPKARNGKSGKRE
                     GKQAGKRSEKNGKKNKRYSDSKQTFGKKKGKKPFYKGVKKKKKKK"
     gene            8287..8751
                     /gene="smpB"
                     /locus_tag="ACG842_001724"
     CDS             8287..8751
                     /gene="smpB"
                     /locus_tag="ACG842_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HFQ0248267.1"
                     /translation="MPKGEGKLIAQNKKARHDYSIIDTMEAGMVLQGTEIKSIRNSRI
                     NLKDGFIRVRNGEAFLHNVHISPYEQGNIFNHDPLRTRKLLLHKKQIVRLENELKNTG
                     ITVVPLKVYIRNGYAKVLIGLAKGKKSYDKREDLKRKDIDRQIDRTLKNFSR"
     gene            9053..9772
                     /gene="atpB"
                     /locus_tag="ACG842_001725"
     CDS             9053..9772
                     /gene="atpB"
                     /locus_tag="ACG842_001725"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HFQ0248268.1"
                     /translation="MDERSLTFHIGPIWFDGTVCIMVLLTCLIVFFLAYFFTRNLKLK
                     PTGKQNAIEWVIDFSRGIVTDNLPRKELSNFHLLAFTLFLFVFVANNIGLVTKIVLPN
                     ETTLWKSPTADPFVTLTLAFIMILLTHLFGVKKLGFKGYFVNSFLKPYSFMFPMKLIE
                     EFTNLLTLALRLYGNIYAGEVLLTLIAKMMTSLGWFSLPLAIPLEMVWIAFSLFIGSI
                     QAFVFVTLSMVYMSHKIEVEE"
     gene            9825..10040
                     /gene="atpE"
                     /locus_tag="ACG842_001726"
     CDS             9825..10040
                     /gene="atpE"
                     /locus_tag="ACG842_001726"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HFQ0248269.1"
                     /translation="MNYIAAAIAIMGAAIGAGYGNGQVISKTIESMARQPEMSGQFRT
                     TMFIGVALVEAVPILGVVIALILVFAV"
     gene            10131..10655
                     /gene="atpF"
                     /locus_tag="ACG842_001727"
     CDS             10131..10655
                     /gene="atpF"
                     /locus_tag="ACG842_001727"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HFQ0248270.1"
                     /translation="MLNQLAIAEVGNAMLGNIIVVSGSFLILLALLKHFAWGPISDIL
                     KKREDKIANDLDSAEQSRINSAKMEQEREQQLLASRSDAADIIKNAKESGELSRQNIL
                     KDAQEEAARLKSKAQADITVERDSALNSVKDDVAELSLQIAEKILNKELSPEMHESLI
                     NQYIEGLGSSNETR"
     gene            10642..11184
                     /locus_tag="ACG842_001728"
     CDS             10642..11184
                     /locus_tag="ACG842_001728"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HFQ0248271.1"
                     /translation="MKLDKYTVGRRYGKALFELAIDSNQAEEIYQDLVSLRQIYEQVP
                     GLGNMLSDVRLEPHEKREIMDKLVSGYDGIVKNFLEVVYEYNRMDDLLFMIEEYERRY
                     DEHQGLLLGSVTTAVPLSDEQRIQLEKNVAKTMDYQSVELKQIVDSSIIGGAIVEANH
                     RVIDGSIRTQLEKMRNQLNR"
     gene            11207..12763
                     /gene="atpA"
                     /locus_tag="ACG842_001729"
     CDS             11207..12763
                     /gene="atpA"
                     /locus_tag="ACG842_001729"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HFQ0248272.1"
                     /translation="MAIKAEEISALIKEQIENYQNVLSVEEIGTVTYVGDGIARAHGL
                     ENAMSGELLEFSNGSYGMAQNLESNDVGIIILGDFESIREGDKVKRTGKIMEVPVGEA
                     LIGRVVNPLGQPIDGLGEIVTDKARPVEAMAPGVMQRKSVNEPMQTGLKAIDALVPIG
                     RGQRELVIGDRKTGKTSIAIDTIINQKGQDMICIYVAIGQKDSTVRTQVETLKKYGAM
                     DYTIVVNAGASQPAPLLYIAPYAGTAMGEEFMYNGKHVLIIFDDLSKQAVAYRELSLL
                     LRRPPGREAYPGDVFYLHSRLLERAAKLSDDLGGGSMTALPFVETQAGDISAYIPTNV
                     ISITDGQIFLESDLFYAGTRPAVDAGLSVSRVGGSAQIKAMKKVAGTLRLDLASYREL
                     EAFTQFGSDLDAATQAKLNRGRRTVEILKQKLHAPLAVEKQVVILYALTHGFLDSIPV
                     DSILDFEHELFEYLDTNHSDIFETIRTTKDLPEEETLNSAIQEYKDMFLATKGNNSST
                     EDKLKSIQNA"
     gene            12777..13679
                     /locus_tag="ACG842_001730"
     CDS             12777..13679
                     /locus_tag="ACG842_001730"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HFQ0248273.1"
                     /translation="MGASLNEIKTRIASTKKTSQITRAMQMVSASKLTKSEASSQKFQ
                     VYATKVREIVTHLTATQLNDIASENPRGDINYNSMLLSRPVKKTGYIVITADGGLVGG
                     YNSSILKQTMSILEEDHQSPDDYVMIAIGGTGADFFKARGINLAYELRNLSDQPSFDE
                     VRKIVNMATTMYQNEVFDELYVCYNHHINSLTSQFRVEKMLPISDLDPEEATTFEQEY
                     IFEPSKEEILSQLLPQYAESLIYGAIVDAKTAEHAAGMTAMKTATDNAANIIDELTVS
                     YNRARQGAITQEITEIVAGASALE"
     gene            13702..15108
                     /gene="atpD"
                     /locus_tag="ACG842_001731"
     CDS             13702..15108
                     /gene="atpD"
                     /locus_tag="ACG842_001731"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HFQ0248274.1"
                     /translation="MSSGKIVQVIGPVVDVEFSLDQSLPDINNALVVYKNDENKSKVV
                     LEAALELGDGVIRTIAMESTDGLQRGMEVIDTGKAISVPVGKETLGRVFNVLGDTIDL
                     ETPFPEDAERSEIHKKAPAFDELSTSTEILETGIKVIDLLAPYLKGGKVGLFGGAGVG
                     KTVLIQELIHNIAQEHGGISVFTGVGERTREGNDLYYEMKDSGVIEKTAMVFGQMNEP
                     PGARMRVALTGLTIAEYFRDVEGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTKKGSITSIQAIYVPADDYTDPAPATAFAHLDATTNLERKLTE
                     QGIYPAVDPLASSSSALAPEIVGEEHYKVATEVQHVLQRYRELQDIIAILGMDELSDQ
                     EKVLVSRARRVQFFLSQNFNVAEQFTGLPGSYVPVEETVKGFREILEGKYDDLPEEAF
                     RSVGRIEDVVEKAKTLGY"
     gene            15122..15544
                     /locus_tag="ACG842_001732"
     CDS             15122..15544
                     /locus_tag="ACG842_001732"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HFQ0248275.1"
                     /translation="MDQYLTVNVVTPAGLVYDHHAAIVVARTTAGELGILPKHAPIIV
                     PLTIDEVRVKRTDSDTHVDWIAVNGGIMEVRDNVVSIVADSAERERDIDVSRAERAKQ
                     RAERQIEEAKAKQDTNELKRATVALHRAINRIKVSKHS"
     gene            16013..16243
                     /locus_tag="ACG842_001733"
     CDS             16013..16243
                     /locus_tag="ACG842_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 family protein"
                     /protein_id="HFQ0248276.1"
                     /translation="MQVYGIDAVLRIVSHLAFIYLAFWSLRSLRIETFFRPMHNTQIR
                     MAIVLFSIFLGYTASNFFLEVIALCRNLFVSL"
     gene            16340..17641
                     /gene="murA"
                     /locus_tag="ACG842_001734"
     CDS             16340..17641
                     /gene="murA"
                     /locus_tag="ACG842_001734"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HFQ0248277.1"
                     /translation="MEEIIVRGGNQLNGTVRIEGAKNAVLPILAASLLAEEGITTLDN
                     VPILSDVFTMNQVIRHLNVDVDFDEQKNQVTIDASRQLEIEAPYEYVSQMRASIVVMG
                     PLLARNGHAKVAMPGGCAIGKRPIDLHLKGFQALGAKIIQKNGYIEAIADELIGNTIY
                     LDFPSVGATQNIMMAAVKAKGTTIIENVAREPEIVDLANILNKMGAQVYGAGTETMRI
                     EGVDHLHAVNHSIVQDRIEAGTFMVAAAMTQGNVLIADAISEHNCPLISKLIEMGAEI
                     IEEEGGVRVIGPKHILPTDVKTMPHPGFPTDMQAQMTAIQLVAEGTSVVTETVFENRF
                     QHLEEMRRMNAHVKIDGNVAIMDGNHELQGAEVYATDLRAAAALVLAGLKANGITRVR
                     NLNYLDRGYYNFHIKLQQLGADVERVDMDQTSAEKTAQTIA"
     gene            17654..17830
                     /locus_tag="ACG842_001735"
     CDS             17654..17830
                     /locus_tag="ACG842_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HFQ0248278.1"
                     /translation="MSNQRYILLTLIKILVVILLLILLFVAGTMIGYGVIGGGNPFKV
                     FQPSLWIHIRDFFH"
     gene            17965..18261
                     /gene="yidD"
                     /locus_tag="ACG842_001736"
     CDS             17965..18261
                     /gene="yidD"
                     /locus_tag="ACG842_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HFQ0248279.1"
                     /translation="MKKILIYLVRGYQRFISPAFPPSCRYYPTCSNYMIKAIQTHGAL
                     KGTVMGTARILRCHPFVKGGIDYVPLTFRLTRNKKDKKEPDYKKVVKNTGSTER"
     gene            18325..18708
                     /locus_tag="ACG842_001737"
     CDS             18325..18708
                     /locus_tag="ACG842_001737"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0248280.1"
                     /translation="MIEELNKKDLPWSLLLDADPDKEKVQVYVSRGSGWIWKEKEKTI
                     GVLIYVAREKEYEIVNVAVTPTYQGKGIGGKLLETAFQKLSQLASNQTRIIIRTGTTS
                     SAALHLYQKMGYVEIGRVKDYFIHH"
     gene            18877..19761
                     /locus_tag="ACG842_001738"
     CDS             18877..19761
                     /locus_tag="ACG842_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFQ0248281.1"
                     /translation="MGILVFDFGGSAVKYGCWDGKEIKGKGQFATPESWEEMKTQLFQ
                     VYKKILVSFEVEGIGISSPGVVNNEKQVIEGISAIPYIHGFNIFRDLETLFQLPVTIE
                     NDANCAGMAEFYEGAARDYQDVAFVVAGTGIGGALFTKGQINKGAHLYGGEFGLMFLD
                     GDKTFSKLGTAVQMAWRYCERKGLDKNAYTGKDVFELAETGDAIAKEEVESFYTYLTK
                     GLFSIQFSFDPEVIVLGGGVSAKEGLIDEIKSRMKKLTEQFDLHDFEPQILLCEYRND
                     ANLVGAAANYLAVRQGEN"
     gene            19875..20111
                     /locus_tag="ACG842_001739"
     CDS             19875..20111
                     /locus_tag="ACG842_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2969 domain-containing protein"
                     /protein_id="HFQ0248282.1"
                     /translation="MMSKKNKDIEVRIEEVKKDIKGKNYEVTQLFIGKKMIGEILAYG
                     PKEYEIFFGEEDFGKEKSLENAIETVIRHWNLHE"
     gene            20199..20279
                     /locus_tag="ACG842_001740"
     tRNA            20199..20279
                     /locus_tag="ACG842_001740"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:20233..20235,aa:Tyr,seq:gta)
     gene            20286..20357
                     /locus_tag="ACG842_001741"
     tRNA            20286..20357
                     /locus_tag="ACG842_001741"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:20318..20320,aa:Gln,seq:ttg)
BASE COUNT         6790 a         3584 c         4444 g         5647 t
ORIGIN      
        1 ttttttgcaa ctagcttctg tatatgatgt ttacaaagaa tggtacaaga aaagatcatc
       61 cttgaaaaag gaaagctaac aataactagc atcatataag agcttttaag gatagaacag
      121 tagaatagga atctatgggc aagtcctctc gtataaataa aataggaggg aagtagcagc
      181 atatttttca taaagtggct ctctttcttc atttttactt tggttgctcc tatttgaaaa
      241 ttcgagcaca ctcaaataca ataaaaagaa atggcaaggg agaggattag atgaaaaaag
      301 aagaaaaaat cgcgatttta caagagatta tccgaattaa atcagtgaat ggaaacgaag
      361 gtgaagtagc agcctatttg aataagcttt tggcaaggca tgacataaca ggagagattg
      421 tttcttacag agatggtaga gataatttga ttgctcgtta ccaaaagggc caatcaggaa
      481 aagtgttagg cctatctgga catatggatg tcgtggcggc tggtgatgaa tcgtcttgga
      541 cctatgcacc ctttgcagca gaaatccatg ggaaccgttt gtacggacgt ggtgctacag
      601 atatgaagag tggcttggca gcaatggtga ttgccatgat cgaactgaaa gagtcaggaa
      661 aaccatttaa cggaacagtc aaattattag ccactgtcgg agaagaagtg ggtgaactag
      721 gtggggaaca gctcacgaaa gcaggctatg tagatgactt agatgccttg atcatcggag
      781 agccaaccaa ttacagtttg atgtatacgc acatgggatc aatcaactat acagtcacct
      841 cgcacggaaa agaagcgcat agctctatgc cagaccaagg ttataatgca atcaatcacc
      901 taaatgaatt cattacgaaa gcaaatgctg aaatgaatca cctcgctgaa acaatcgaga
      961 atcctgtcct aggaaaaaca atccataatg ttacattgat cagtggcggg aatcaagtca
     1021 acagcatccc aagccatgcg cagctccaag ggaacattcg atcgattcca gaatacccaa
     1081 atgataaaat cattgcattg ctgcaatcaa tcgtcaatga attgaatcaa gagacagact
     1141 atcatttgga attgatgatc gattacaaca aaatacctgt caaagctgat ccagattctc
     1201 ctttgattca ttccattcag caacaattca gccagccatt acctttagtt ggagcggcag
     1261 caacaacgga tgcagccgaa tttacgaaag ctaaccattc cttcgacttt gtcgtctttg
     1321 ggcctggtgt cgttactttg cctcatcaag tagatgagta tgttgaaatc gataattatt
     1381 tggatatgat cgaaaaatat caaggaatta ttttatccta cttagcgtaa gattttttta
     1441 ataaatgaaa aaacagagcc agattagcat tattttgcta atccagctct gtttattcag
     1501 cctatttttc tttttgaata tatcaagcgt tggggcaaca tagtgatgac taatgaaaca
     1561 acgacgatag acaacaagcg atcaaggtag tctgtaccca cctgtaccaa gatcacactt
     1621 gttgtctgac ttaaacctaa cccatgtaat agctgaacga tcatactgga tcctgaggag
     1681 gtgatcccgc caaataactg aacggtaatg atcgaagaaa cgatcgttcc aggtaccgac
     1741 aaaatcaaag cgagtaataa cagtttcaag ctgtttttat ctttgccgag atatcttggc
     1801 aaaatcaatc cagcaagcaa gcctgtgaga atttggattg gcgaataata cagagaaaaa
     1861 atatctgtag tcactccact taaaataccg ctggacaatc ctgtcagcat tccaggtaca
     1921 ggcccaagca atgcaccagc aaaaatcgtt ccgatagaat cgagatagat tggcaaacga
     1981 agccataaag caatcgttcc tccaatataa ttgatcccaa tacacaaagc catcactgta
     2041 agtacgcgag ttgccatttt gtttttattc attttgttag cccatcttta acctttccaa
     2101 atctttcata atcgtttctt cttgtgcatt taaaagaacg gttaaaaact ttcggaaaaa
     2161 aagacgagta tctacttttg tcataatttt actgttagca ggtttctgcc aaaattcata
     2221 acgatcaacc aatgtttgtc cgcgacttat tccttgagtt tctacagcag tgaagctttt
     2281 aaagccttca cagatggact catcgataaa ataagcaaca gctaatggat cattgatgac
     2341 acatcctaag attctttctt gtttccagtg aaagtcaaaa taaaaacgag tgatcgcttt
     2401 tagatactct ccctcttcag gattcatttg gcagcaatat tcaaggatag tcggtgtcaa
     2461 gacgatttct cttgtcacat ctaacccaac catttctatc ggttgtttga gcttttcgaa
     2521 tacataagaa gctgcttcag gatcacacca gtaattgtat tcagcaactg gtgaacagtt
     2581 accatggctt ttgtaagttc cgcccattga gacaaaacga tccatatgtt tcccaacaga
     2641 tgggctgttt ttcaaagcag aagcgatatt ggtcaaaggc cccaaagcaa tgactgatgt
     2701 atctgttttt tctttgaaat aatcagtcag aaaatcaact gcatggagcg attctgcctg
     2761 tttggctaag accatcggga aatttgtgtc ccctaatcca tccaacccat gagtgtcttg
     2821 cgcactaatg aaaggttgct tcaaaggttt ttccattcct tgataaacag ggatatctaa
     2881 acgatcacag cgctccaaac atttcagcac attctgcgta ccgatatcta cagggacatt
     2941 cccgcaaacg acggtaatag caactacttc gatatcaggg gatttgacag ctagtgataa
     3001 agctaaagtg tcgtcgatcc ctggatcgca atcaataatt actttacgca ttatttccaa
     3061 tctcctttct ttagagatac ttagtttttt attctgggaa gtttccgaac cagtccaaga
     3121 cgagtgtctt gtttataatg cacgaaatta gtatactggc ttttattgtg aatgtaaatg
     3181 ctctcttaca agtaaatcta ttgagaagaa ctttaagtaa atgatacaat gaaaaagaaa
     3241 tggaatttac gtgtgccgaa gaaaaagaat cctattgatc ggtatattgt ataataaata
     3301 gattatagtt tggcagaaag cggcgggtgt acgatggtta ttttttattt cattcttatt
     3361 ctgatattta taggaatcag ttaccatttt ctttcttatt tttatgcaaa gaaattagta
     3421 agagcggggg tacagagagg ctatgactgg tatgaagaaa ctgggcaccg cttgatggat
     3481 cctgctgcag tcacgtcagt agaaaaaaag cgacaaaaat tagaagaaga gtgtgaaagc
     3541 ttctgggaac aaggaatacc gaaaacgatc aaaagttatg acgggttgaa actagctgga
     3601 cagatgttcc tccaaccaac tcagcagaat aaatgggtca tttgtgtaca tgattatcga
     3661 agtacaggga aaagagatat gtctcatata ggtaaaagat atgcagagaa aggattcaac
     3721 gttcttatac ccgatttacg tgcgcatgga gaaagtgaag gagaaatcat cggaatggga
     3781 tggctagacc ggctagattt aattgcatgg atccaactca tcttagatga acaaccagat
     3841 gcatccatta ttttgcacgg cagttcgatg ggcgcttcta ctattatgat ggcaagtggt
     3901 gaaaagcttc catctgcagt caaaggattt atcttagaca gcggatatgt ttctgtttat
     3961 gcggaatttc ggtatatgtt aagtaaaatc acggtctttc ctaaaaaaat gatcatgaga
     4021 tatgcgaatc actatgctca gaaatacgca ggttattctt taaagcaagc atcagctaca
     4081 agacagcttg gtagtaacca tttgcctttg ttagtcatcc atggtgaaag agatcatttt
     4141 gttcctacag aagcagcata cacgattcaa aacgctacgg caggggataa agcattactg
     4201 cttgttccag aagcagaaca tttggaagca tcgatgaaag atccgaaaac ctattggaca
     4261 gtgatttttt catttattga acaaagaata acgatggatt gattttctct tcctttttgt
     4321 aagcaacacg aggataaata attgtttata gaccatgaga ctgttaaaat aaggtaggaa
     4381 taaaaaaata tcagttattt gaggagtgcg gcatatggaa atcaaagagg aaaaaaatcg
     4441 tcttgcttta ttcaatgatg agcaacaaga aatcggagaa atgacatggt cggatgcggg
     4501 gccagacatc atgatcattg accatacatt tgtcgatcca gcatatcgtg gtcaaaaatt
     4561 agctgaaaag ctagtgtata ctggagtaga actagcaaga cgagagggga aaaagattat
     4621 cccttcatgc ccttacgcaa aaaaagagtt tgagaagaaa ccagagtatc atgacgtctt
     4681 acgccaatca taataataac agctgaaaga tttgcacttt cagctgtttt tccatttata
     4741 tagagttgtc acggagtttt atctatgata aagttaaaga gacgatatct tttgggaagg
     4801 aggctgctat atgtataatt taattttggg aatcgtgatc gttttatcga ttgttatggt
     4861 cattgcgatt atgatgcagc caagcaagca aaatagtgca gcgagtgcat ttaccggcgg
     4921 tgcggatcaa ctattcggca aacaaaaagc tcgcggcttt gaagctgtca tgcagcgatc
     4981 aactgcagtc atgggagctg tctggatgat cttattattt gtcctagcat ttttatcttc
     5041 gaagtagtcg aataagcctc tcgcacaacg gcgggcggct ttttttattg caaagagaac
     5101 gcttaagaag aagttatgaa agaaactatg gtaaaatgag gcgaaagagg tgaaacgagt
     5161 gaaacaatta ccaaaacccc tttatgcaaa acatggaaaa cgagcagttt tgctgcttca
     5221 tgcgtactca ggaagtccaa atgatgtccg gatgctcgca cggtttttag aaaaagcaga
     5281 ttatacagtc tatgcgccgc tatttaaagg acatggaacg atggagccat atgatatctt
     5341 gcaagaaaaa gcagaaagct ggtgggcaga tactaaaaaa gctatccatt ttttacagtc
     5401 tgaacagttt tctgatattg ccgtattggg attatcgatg ggtggcatct ttgctgtacg
     5461 ggcactagaa gaagaatcag taattggcgg cggttttttc tgttcgcccc tgtctcctgt
     5521 agaaacaaac gtgcctgaaa attttgaaaa atatgtacgt caagtgctga agatagcagg
     5581 caaatcagaa aaagaaatca atgaaaaagc tgtgtcttac cgctcgttag ctgaacatca
     5641 attaatggat atacaagacc aagcagcaat cgcagaaagt agactatcag atatccaaca
     5701 gccaatattt ttagctcaag ctgggaaaga tgaaatgatc gacccaaatg gtgtatttga
     5761 aacggcacgc aaattgagcc gccaacgagt gacacttcaa tggtatcctg aaagcggtca
     5821 tgtgattaca gttggcacag caagacgcga attagaaaaa gacgtgttgg aatttttaga
     5881 aaaattacct tggaatgagg aataaatatg acaaaagaaa caataaaaga gaaaattctc
     5941 tttttcatgg aaagcagtcg caaacgtagt ttttctatgg aagaaatcgc tgaaggtcta
     6001 ggctttcaaa aaagcgatga ttttaaatta ctagtacaaa cgattgctca aatggaaaga
     6061 gaacagagta tcgttttcaa taaaaaagga aaagtcaaac ttcctttaaa accgatcttg
     6121 gtcgaaggga catttcgggc aaatgaacga ggatttggat ttgtaacgat tgatcccgaa
     6181 gaagatgata tctatatccc gaaagaagcg actggctttg caatggatgg ggataccgta
     6241 gcaattgata tcatgaagcg agcaaataca gatatggatc gcggagcaga gggaaaagta
     6301 gtggagattc gagaccgagc gactactcag ttagccggag aatttgttgc ttattcagac
     6361 gaagagatac aggaaacgga tctttatggt gtggtcatcc cgaaagatag aaagcttaac
     6421 caattcaaag tgtatgcagc ggcagaggga gttcgtccag ttgacggaag tattgtattg
     6481 atcgaactga cccattaccc tgaaaaaggg tatgcaacta gtttagaagg gataatcaag
     6541 caagtgattg gtcataagaa tgaccctggc atggatatcc tctcgattgt cgtggctaac
     6601 ggtattccta caaaatttcc tgatgatgtg ttatctgagg cagatgaagt tccagaccag
     6661 atcagcgaaa aggatattgt tggacgcaga gatttaagag atcagctcat cgttacgatt
     6721 gatggagaag atgcaaaaga tctagatgat gcagtgacgg tcaaaaaatt agaaaatggt
     6781 aattattttc ttggagtaca cattgcagat gtatcttact atgtgacgca aggcagtcaa
     6841 ctggacagag aagcatacga aagaggaaca agtgtctatt tgaccgatcg agtgattccg
     6901 atgatcccac aacgtttatc caatggtatc tgttcattga atccccatgt tccacgactc
     6961 accatgagtt gtgaaatgga gatcgatcca aatggacaag ttgtctctca tgagattttt
     7021 ccaagtgtga tccaaacaac agaacggatg acctacacag ctgtcaatca aatcttagaa
     7081 gatcaagatg aagaaacgat gcaacgttat gctcagctcg tgcctatgtt ccaagaaatg
     7141 ggtgaattgc atcgaatcct tgaaatgatg cgaatcaaac gtggagcgat ttcttttgag
     7201 gatcgtgaag cgaagatcct tgttgatgct gaaggacacc caaatgatat ccagctaaga
     7261 agcagaggga taggtgaacg cttgatcgaa tcctttatgc ttgctgccaa cgaaacggta
     7321 gctgaacatt ataataaatt aaatcttccg tttatttatc gaatccatga acagccaaaa
     7381 gaagaaaaaa tgcagcgctt ctttgatttt gcttcagctt tgggcattct ggtccgagga
     7441 acgaaaggaa cgatcacacc aaaagattta cagcatgtga tcgaaggagt agaagacaaa
     7501 ccagaagctg cagttatcaa tactatgttg ctaagaagca tgcaacaagc acgctattca
     7561 gaagacaatt ttgggcatta tggcttagcg gcagattact acacacactt tacatctcct
     7621 attcgacgct atcccgatct aatcgtccat cgtctgatcc gttcttacag tgaagataaa
     7681 agtgaaagca atcaaagttc gtgggctgcg gaacttcctg aaattgcaga tcatagttct
     7741 aagatggaac gtcgtgcagt agaaacagaa cgtgaagtag attccatgaa gaaagcagaa
     7801 ttcatgatgg acaaagtggg agaagaattc gatgggatca tcagctctgt tgtgaaattc
     7861 ggcctgttta tcgaactgcc gaatacagta gaaggattga tccatatcaa tgaattgaaa
     7921 caggactact tccattttgt tgaaaatcat ttagctcttg taggtgaaag aactggtctg
     7981 acttttaaaa tcggacaaaa agtgcgcgta aaagtagtca aagctgatcc agatgaacga
     8041 gcagtggatt ttgaactcgt ttccgcagaa gaggtcactg cgttggaacg tccaaaagca
     8101 cgcaatggaa aatctggaaa acgagaagga aaacaagcag gcaaacgctc agaaaaaaat
     8161 ggcaagaaga acaaacgata tagtgatagt aagcaaacgt tcggtaagaa aaaagggaaa
     8221 aagccatttt acaaaggtgt caaaaagaag aaaaagaaaa aataaaatga gttggaggaa
     8281 acagctatgc caaaaggcga gggaaaacta attgcacaaa acaagaaagc tcgccatgat
     8341 tattcgatca tcgacacgat ggaagcaggg atggtcttgc aaggaacgga gatcaagtcg
     8401 atacgaaaca gccggatcaa tctgaaagat ggatttattc gcgtccgcaa cggggaagct
     8461 ttcttgcata atgttcatat cagtccttat gaacaaggaa atatttttaa tcatgatccg
     8521 ttgcgcacga gaaagttatt attacacaag aaacaaatcg ttcgtcttga aaacgaattg
     8581 aaaaacactg gaatcactgt tgttccttta aaagtctata ttcgtaacgg ctatgccaag
     8641 gtattgattg gtctggcaaa agggaaaaaa tcgtatgata aacgggaaga tttgaaacga
     8701 aaagatatcg atcgacaaat tgatcgaaca ttaaaaaatt tctctagata atttcattat
     8761 cctttttcgc atattcggtg cttttgcttt tacttaatga taaaatttgt tagcatttaa
     8821 tctgataaaa catgattatc acaggttttt tcttagatca ggagcaccgt gcatggggaa
     8881 aaattcgctt cgtttttgac tgaaataaaa aagtgtctaa ttttcatgaa aatgttttga
     8941 attttctgtt tcatggtggt atgataccgt gtggttagaa accgattgga aaaaaccaga
     9001 tacgcacatg aattgatttt atgtgaattt cggggaaaag aggtgaaatg gattggatga
     9061 acgctcgcta acgtttcaca ttggaccaat ttggtttgat ggtacagttt gtataatggt
     9121 gttactgacc tgtttgattg ttttcttctt agcttacttt tttacaagaa atcttaagtt
     9181 gaaaccaaca ggcaaacaaa atgcgataga atgggtcatt gattttagtc gaggaatcgt
     9241 aacagataat ctgcctagaa aagaattgag caatttccat ttgttggcat ttacattgtt
     9301 tttatttgtt tttgttgcaa acaacatagg tttagtaaca aaaatcgtct tacctaacga
     9361 aacaacactt tggaaaagcc cgacagcaga cccgtttgtt actttaactt tggcatttat
     9421 aatgatctta cttactcact tattcggggt gaaaaagtta ggcttcaaag gatatttcgt
     9481 aaattcgttc ttgaaaccct atagttttat gtttccgatg aaactgattg aagaatttac
     9541 aaacttatta acacttgctt tacgtcttta tgggaatatt tatgcggggg aagttttgct
     9601 aaccttgatt gcaaaaatga tgacaagctt aggctggttc tcattaccat tagcaattcc
     9661 attagagatg gtctggatcg cgttctcctt gtttattggg agcatccaag catttgtctt
     9721 tgtcactttg tcaatggttt atatgagcca taagattgaa gtagaagaat aatactcggt
     9781 taaaaaacaa actactattt atctatttta ggaggaaaac aattatgaat tatatcgcag
     9841 cagcaatcgc aatcatggga gcagcaatcg gagccggtta tggtaacggt caagttatct
     9901 ctaaaacaat cgaatcaatg gctcgccaac ctgaaatgtc tggtcaattc cgtacaacaa
     9961 tgtttatcgg ggtagctcta gttgaagctg ttcctatctt aggggtagtt atcgccttga
    10021 ttctagtttt tgccgtttaa ttgaatcagt attaaggcag aaacaacatc gtttgttact
    10081 gcttttttac atggaaacta gaaatactcc aaagaaagga aggctcgcct atgctgaatc
    10141 aattggcaat tgcagaagtt ggcaatgcga tgttaggtaa tatcatcgtt gtcagcggct
    10201 catttttgat actactggct ctcttgaagc actttgcttg gggaccaatc agcgatattt
    10261 tgaaaaaacg tgaagacaag atcgccaatg atttagattc tgcagaacaa tctcgcatca
    10321 actcagcgaa aatggaacaa gaacgcgaac aacaattgtt agcctctcgt tctgatgcag
    10381 ctgatatcat caaaaatgcg aaagaaagtg gagaattaag ccgccaaaat attttgaagg
    10441 atgctcaaga agaagcagct cgtctaaaaa gcaaagccca agctgatatc actgtagaac
    10501 gtgattcagc gctgaactct gtaaaagacg acgttgcaga actctctctt caaatcgcgg
    10561 aaaaaatctt gaacaaagaa ttatctccag aaatgcatga atcattaatc aatcaataca
    10621 ttgaaggtct aggttcttca aatgaaacta gataaatata cagtaggaag acgttacggg
    10681 aaagctttat ttgaactggc gatcgattca aatcaagcag aagaaatcta tcaagatctc
    10741 gtaagtttac gtcaaatcta tgaacaagta cctggattag ggaatatgtt aagtgatgtt
    10801 cgtctagaac ctcatgaaaa acgagaaatc atggacaagc ttgtcagcgg ttatgatgga
    10861 atcgttaaaa actttttaga agttgtttat gagtataacc gaatggatga ccttttgttc
    10921 atgattgaag aatatgaacg tcgatatgat gaacatcaag ggttactatt gggaagtgtc
    10981 acaacagcag tcccactttc agacgaacaa cgaatacaat tagaaaagaa tgttgcaaaa
    11041 acaatggact atcaatcagt tgaattaaaa caaatcgttg attcttctat tattggtggc
    11101 gcgattgttg aagccaacca tcgagtaatc gacggaagca ttcgcacgca attagaaaaa
    11161 atgcgtaatc aattaaacag atagtacaaa gaggtaggtg aatcgaatgg ccatcaaagc
    11221 agaagaaatt agtgccttga ttaaagaaca aattgaaaat tatcaaaacg tgctttcagt
    11281 cgaagaaatc ggtaccgtaa cttatgtagg tgacgggatc gctcgtgcac atggtttaga
    11341 aaacgcgatg agtggtgagt tgcttgaatt ttcaaacggc tcatacggaa tggcgcaaaa
    11401 cttagagtca aatgatgtag gtatcattat ccttggcgat ttcgagtcca ttcgtgaagg
    11461 agataaagta aaacgaacag gtaagatcat ggaagttcca gttggagagg ccttgatcgg
    11521 tcgggtagtc aatccgctag gtcaaccaat cgacggacta ggtgaaatcg ttactgacaa
    11581 agctcgtcca gtagaagcga tggcaccagg tgttatgcaa cgtaaatctg ttaacgaacc
    11641 aatgcaaaca gggctaaaag cgatcgacgc ccttgtgcca atcggacgcg gacaacgtga
    11701 attagtcatc ggtgaccgta aaacagggaa aacttctatt gcgattgata cgatcatcaa
    11761 ccaaaaaggt caagatatga tctgtatcta tgtagcaatc ggacaaaaag attctacagt
    11821 tcgtacacaa gttgaaacat tgaaaaaata tggcgcaatg gattacacaa tcgttgtgaa
    11881 tgccggtgcg tctcaaccag caccattact ttatatcgca ccatatgctg gtactgcaat
    11941 gggtgaagaa ttcatgtaca acggtaaaca tgtattgatc atttttgatg atctttcaaa
    12001 acaagctgtt gcttatcgtg aactttcctt gctacttcgc cgtccaccag gtcgtgaagc
    12061 ttatcctggg gatgttttct acttgcactc acgtttatta gaacgtgcag caaaattaag
    12121 tgatgatcta ggcggcggtt caatgactgc tttgccattt gttgaaacac aagctggaga
    12181 tatctctgct tatatcccaa caaacgttat ctcaatcact gacggacaaa tcttcttaga
    12241 aagcgacttg ttctacgcgg gtactcgtcc agcggttgat gccggactat ctgtctctcg
    12301 tgtagggggt tctgcgcaaa tcaaagcaat gaaaaaagtt gctgggactt tacgtctaga
    12361 cttagcaagt tatcgtgaat tagaagcatt tacacaattc ggttctgatt tggatgcggc
    12421 aacacaagca aaactaaatc gtggacgccg tacagtagaa atcttgaaac aaaaactgca
    12481 tgcaccgcta gctgttgaga aacaagtagt gattttgtat gccttgacac atggattctt
    12541 ggacagtatc cctgtagaca gtattttaga cttcgaacat gaattattcg aatatctgga
    12601 taccaatcat tcagatattt ttgaaacaat ccgtacaaca aaagatcttc cagaagaaga
    12661 aacgttgaac agtgcgattc aggaatataa agacatgttc ttagctacaa aaggaaataa
    12721 ttcctctaca gaagataaat tgaaatccat tcaaaatgca tagtgaggtg agatgaatgg
    12781 gtgcttcatt aaatgaaatc aagacacgta tcgcttcaac gaaaaaaacg agccaaatca
    12841 cgcgggcaat gcagatggtg tctgcttcta agttgacaaa atctgaagct tcttcacaga
    12901 agtttcaggt ttatgcaaca aaagtaagag aaatcgttac gcatttgact gctactcagt
    12961 taaatgatat tgcctcagaa aatcctagag gggatatcaa ttacaacagc atgctgctca
    13021 gccgtcctgt gaagaaaacc ggttatatcg tcatcactgc agatggcggt ctggtcggtg
    13081 gatataacag ttcgatattg aaacaaacga tgtctatttt agaagaagac caccaatcac
    13141 ccgatgatta cgtgatgatc gcaattggcg gaactggtgc agacttcttt aaagcacgcg
    13201 gtatcaacct agcatatgag ctgcgcaatt tatctgatca gccaagcttt gatgaagtcc
    13261 gcaaaatcgt aaatatggca acaactatgt accaaaacga agtattcgat gaattatacg
    13321 tatgttataa ccatcatatc aattcgctaa ctagtcagtt tcgtgtagaa aaaatgctgc
    13381 caatctcaga cttagatcca gaagaagcaa cgactttcga acaagaatat atttttgaac
    13441 cttcaaaaga agaaatcttg tctcagttat tgcctcagta tgctgaaagt ttgatctacg
    13501 gtgcgatcgt tgacgccaaa acagcagaac atgctgctgg tatgacagca atgaaaacag
    13561 caactgataa tgctgctaat atcattgatg agctgacggt ttcttataac cgtgcaagac
    13621 aaggggctat tacccaagaa attaccgaaa ttgttgccgg agcttcggca ctagaatagt
    13681 attgagattg gaggaaaaaa gatgagttca ggcaagattg ttcaagtaat cggtcccgtt
    13741 gtcgacgtgg aattttcttt agaccaatcc ttaccagata tcaacaacgc attagttgtt
    13801 tataaaaatg acgaaaataa atcaaaagtt gttcttgaag cagcgttaga attaggggac
    13861 ggagtgatcc ggaccattgc gatggaatca acagatggtt tacaaagagg aatggaagtc
    13921 attgatacag gcaaagcaat ctctgttcct gtaggtaaag aaacattagg tcgcgtattc
    13981 aacgtactag gagatacgat cgatttagaa acacctttcc cagaagatgc ggaaagaagc
    14041 gaaattcata aaaaagcacc agcctttgac gaattaagta caagtacaga aattttggaa
    14101 acagggatca aagttatcga tttgcttgcc ccatatttaa aaggtgggaa agtcggacta
    14161 ttcggtggtg ccggtgttgg taaaaccgta ctgatccaag aactgatcca taatatcgcc
    14221 caagaacatg gtggtatttc tgtatttacc ggtgtaggtg aacgtactcg tgaaggtaat
    14281 gacttgtatt atgaaatgaa agattcagga gttatcgaaa aaacagccat ggtgttcgga
    14341 caaatgaacg aaccaccagg tgcacgtatg cgtgttgctt tgactggttt gacgattgcg
    14401 gaatatttcc gtgatgtaga aggtcaagat gtactgttgt ttatcgacaa cattttccgt
    14461 ttcactcaag ctggatctga agtatcagcc ttgttaggac ggatgccttc tgcggttggt
    14521 tatcaaccaa cattggcaac agaaatgggt caattgcaag aacgtatcac atctacgaaa
    14581 aaaggttcga tcacatcgat ccaagcaatc tatgtgccag ccgatgacta cactgacccg
    14641 gcgccggcaa cagcattcgc ccatttggat gcgacaacca acttggaacg taaattaaca
    14701 gaacaaggga tttatccagc ggtagatcca ttggcttctt cttcaagtgc tttagcacct
    14761 gaaatcgtgg gagaagaaca ttataaagta gcaacagaag tccaacacgt attgcaacga
    14821 tatcgtgaat tacaagatat cattgctatc ttaggtatgg atgaattatc cgatcaagaa
    14881 aaagtgttag tttctcgtgc ccgcagagtc caattcttct tgtcacaaaa ctttaacgtg
    14941 gctgaacaat ttactggtct tccaggttca tatgtccctg tagaagaaac agtgaaagga
    15001 ttccgcgaaa tcttggaagg caaatacgat gatcttcctg aagaagcttt ccgaagtgtt
    15061 ggccgaatcg aagacgtcgt agaaaaagcg aaaacactag gctactagaa aggggtgccc
    15121 catggatcaa tatttaacag taaatgtggt aactcctgcc ggtttggtct atgatcacca
    15181 tgcagctatc gtcgttgcac gtacgacagc tggtgagctt gggattttgc ctaaacatgc
    15241 accaatcatc gttccattga cgattgatga agttcgtgta aaacgaacag attcagatac
    15301 ccacgtcgat tggatcgctg ttaatggcgg aatcatggaa gttcgtgata atgttgtttc
    15361 aatcgttgct gatagtgcgg aacgtgaacg tgatatcgat gtatctcgtg cggaacgtgc
    15421 aaaacaacgc gcagaacgcc aaatcgaaga agcaaaagca aaacaagata cgaacgaatt
    15481 gaaacgggca actgttgcgt tgcaccgagc aatcaatcgt atcaaagtat ctaagcactc
    15541 ataaaatgag atgcggatgt ttatccgtgt ttaagaacag taaaaagagg ttatgacaga
    15601 agtggtcagc ttcaagacat aagaaggaat ttacgaaaat tgcttctcaa atttttgtga
    15661 aatctcagct tatttttcga agaagctact tctggaacac cgtatatcag aggttgtgaa
    15721 atcagcgttt tgtcctgagt attagagaaa caaatggaaa atagttcctc atattttgcc
    15781 gttttttgag ctaataccga gggtcagatg attgaacacc gtttatttgg aataagggac
    15841 tgcgacaaga cttttgtctc agtccctttt tttgcatatt tttagcattt atttcctaaa
    15901 aaatgtaagg ggattcctat atttttaagt atacttgttt ttccttttat acattttatg
    15961 gtattatcat tttattagtt attataaaac aatcgttaga aaggacgtca ttatgcaagt
    16021 atacggaatt gatgcggtgc ttcgaatcgt gagtcatttg gcgtttatct acttagcctt
    16081 ctggtcttta cgttcgttac gcattgaaac ttttttcaga ccaatgcata atacgcaaat
    16141 tagaatggca atcgtacttt tttcaatttt tttagggtac actgcaagta attttttctt
    16201 agaagttatc gctttatgcc gaaatctttt cgttagcctg taaattttaa aaaaaggtac
    16261 tattttaaaa gcaaggatgt tataatagaa aagattttga gtaagtgtat gctcattgga
    16321 attttttgga gggaacaaca tggaagagat catcgtaaga ggtggcaatc aattaaatgg
    16381 aacagtaaga attgaaggag ctaaaaatgc tgttttgcct attttagctg ctagtttgtt
    16441 agcagaagaa ggtattacga cattagacaa cgttccgatt ctttctgatg tcttcactat
    16501 gaatcaagtg attcgtcact taaacgtaga tgtagatttt gatgaacaaa agaatcaagt
    16561 aacaatcgac gcttcaagac aattagaaat cgaagcgcct tatgagtatg ttagtcaaat
    16621 gcgtgcttca atcgttgtga tgggtccgct acttgccaga aatggacatg caaaagtagc
    16681 tatgcctggt ggatgtgcga ttggtaaacg accaatcgat ttgcatttaa aaggatttca
    16741 agctttagga gcaaaaatta ttcaaaaaaa cggctatatc gaagcaattg cggatgaatt
    16801 gatcggtaac acgatttatt tagatttccc tagtgtgggt gctactcaaa atatcatgat
    16861 ggctgccgta aaagcaaaag gaacaaccat cattgaaaac gtagctcgcg aaccagaaat
    16921 cgttgattta gcaaacattt tgaataaaat gggcgctcaa gtctatggag ctggtactga
    16981 aacaatgcgt attgaaggcg tggatcattt acatgcagtc aaccactcaa tcgtacaaga
    17041 tcgtatcgaa gcaggtacct ttatggttgc tgctgcaatg acacaaggca atgtgctgat
    17101 tgcagatgct atctctgaac ataactgccc attgatttct aaattgatcg aaatgggtgc
    17161 agaaatcatt gaagaagaag gcggagtccg tgtcatcgga ccaaaacata tcttacctac
    17221 agatgtaaaa acgatgcctc atcctggttt ccctacagat atgcaagctc aaatgacagc
    17281 tatccaatta gttgcagaag gtacaagtgt ggtgactgaa acagtgtttg aaaatcgttt
    17341 ccaacattta gaagagatgc gccgtatgaa cgctcatgtc aaaatcgacg gaaatgtagc
    17401 aatcatggat ggaaaccatg aattgcaagg tgcagaagta tatgcaacag acttgcgggc
    17461 agcagcagca ttagttttag ctggcttgaa agcaaatgga atcacacgtg tgcgtaactt
    17521 gaattatcta gaccgtggat attataattt ccatatcaaa ctacagcaat taggtgcaga
    17581 cgtcgaacgt gtagacatgg atcaaacttc tgctgaaaaa acagcacaaa cgattgctta
    17641 aggaagtggc tgaatgagta accaacgata tatcttatta actttgataa agattttagt
    17701 ggtgatctta ttgcttatcc ttctttttgt ggccggaacg atgatcggat atggtgtcat
    17761 cggtggcggc aatccattta aagtattcca gccaagtttg tggattcata ttcgagactt
    17821 ttttcattga taaataagaa tcacagtggt tctagtcgat atgaagtgag ccatgaagca
    17881 gaacatgata aaagaaaaca gaagggaacg ttgacgttag tcgcgatccc tttttttagt
    17941 aaaagaaaag aaggaatcta attgatgaag aagatattga tttatttagt tagaggatat
    18001 cagcgcttta tttcgcctgc atttccacct agctgccgtt attacccaac ctgttccaat
    18061 tatatgataa aagcaattca aacccatggt gcgctaaaag ggacggtaat ggggactgca
    18121 cggatcttac gctgtcatcc ttttgtcaaa ggcggtatcg attatgtacc attgacattc
    18181 cgattaacgc gaaataagaa agataaaaaa gaaccagatt ataaaaaggt tgtgaaaaat
    18241 acgggtagca ctgagcgata aagaaaaaga ggaaatttat tgtcaaagta acaactccgc
    18301 aactacttat atgggggaca aaaaatgatc gaagaactaa acaagaaaga tttaccatgg
    18361 tccttgttat tagatgcaga tcctgataaa gaaaaagttc aagtatatgt ttccagaggg
    18421 agtggctgga tatggaaaga aaaagaaaaa acaataggtg tactgatcta tgtggcaagg
    18481 gaaaaagaat acgaaatcgt caatgtagca gtaaccccaa cgtatcaagg aaaggggatt
    18541 ggcggaaaat tacttgagac agcttttcag aagctttcac agctagcttc aaaccaaact
    18601 cggatcatta tacgtactgg cactactagt agcgctgcgt tacacctcta tcaaaaaatg
    18661 ggctatgtgg agattggtcg agtcaaagac tattttatcc atcattaatc tgagcccatt
    18721 tttgaaaatg gagatcaatt aagggatcaa gtgattttag caattcactt acctaaaaat
    18781 agctttaact aataaacttt caattcgaga aaatgctttc aaatgctaaa tagatacatt
    18841 acaatagctt tagcaaacaa aaaggagaat atttttatgg gaatattagt ttttgatttt
    18901 ggtggatcgg ctgtcaaata tggttgttgg gatggcaaag agattaaagg taagggacaa
    18961 tttgcaacgc ctgaatcttg ggaagaaatg aaaacgcaat tatttcaagt ttataaaaaa
    19021 atactcgttt cttttgaagt cgaaggaatt ggaatcagct ctcctggagt agtgaataat
    19081 gagaaacagg tgattgaggg aatcagtgcg attccttata ttcatggatt taatattttc
    19141 cgagatttgg aaactctgtt tcaactccca gtaacgattg aaaatgacgc caactgtgcc
    19201 ggaatggctg aattttatga aggtgctgcc cgtgattatc aagatgttgc ttttgttgtg
    19261 gcaggaactg ggattggcgg ggcgttgttc acgaaaggac aaataaataa aggggcacat
    19321 ctttacggtg gagaatttgg gttgatgttc ttagatggcg ataagacatt cagtaaactt
    19381 ggtacagcgg tacagatggc ttggcgttat tgtgaacgca aaggtctcga taaaaatgct
    19441 tataccggaa aagatgtctt tgaattagca gaaactggag atgcaatagc aaaagaagaa
    19501 gttgagagtt tttatacgta tcttacaaag ggattattca gtattcaatt ttcgtttgat
    19561 ccagaagtga tcgtattagg cggcggggtt tctgcaaaag aagggctcat tgatgagatc
    19621 aaaagccgaa tgaagaagct gacagaacaa ttcgatctgc atgatttcga accgcagatt
    19681 ttattatgtg agtacagaaa cgatgctaat cttgtaggtg cagcagcaaa ctatctcgct
    19741 gtacgtcaag gagagaatta agagctttag agaaggtgca gcttgcataa aagaagagaa
    19801 tgaaatgaat aggagatttt tacctaagga atgttttatt tgctgcagta tgataaaata
    19861 tctgagaggt gaaaatgatg tcaaaaaaaa ataaagatat tgaagtgcgt atagaagaag
    19921 tgaaaaaaga tatcaaaggt aagaattatg aagtcactca gttatttata gggaaaaaaa
    19981 tgattggtga gatcttagcc tatgggccaa aagaatatga aattttcttt ggtgaagaag
    20041 attttggaaa agaaaaaagt cttgaaaatg cgatagaaac agtgattcga cactggaatc
    20101 tgcacgaata aaaaaatatc aaaaaaactt tcaataaagg gttgcaaaaa tctggaaaat
    20161 taggtattat aactaagtca ggttgataca aacctttcgg aggagtagcg aagtggctaa
    20221 acgcgacgga ctgtaaatcc gttccttagg gttcagtggt tcgaatccac tctcctccat
    20281 ttcattgggg tatagccaag cggtaaggca agggactttg actccctcat gcgttggttc
    20341 gaatccagct accccagtat accggctctt tgtcaataag gactgatgag ttgtgcaaaa
    20401 tcaaatctga gtcagaatga accacattct ggctcagatt ttttgttatc ttacattgat
    20461 taatt
//
LOCUS       DAZKLM010000054        19190 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3764
            SAMEA115706913-rid22582563.denovo.055, whole genome shotgun
            sequence.
ACCESSION   DAZKLM010000054 DAZKLM010000000
VERSION     DAZKLM010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706913
            Sequence Read Archive: ERR13196647
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19190)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19190)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287823.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287823.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,004
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,849
            CDSs (with protein)               :: 2,849
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 41 of 96
            Pseudo Genes (incomplete)         :: 52 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 15 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19190
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706913-rid22582563.denovo.055"
                     /isolate="VRE3764"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            136..1563
                     /locus_tag="ACG842_001742"
     CDS             136..1563
                     /locus_tag="ACG842_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0248283.1"
                     /translation="MIEEYIEKNILRQLFLCGQFYVNKEVNLEKLSNLLHVCKTTLLN
                     DINNIKKEFEEQIAYTHREKDRYTLYFSEHIPRCKIMQQLSQNSLFLKTCLLYLEEDE
                     PDYLQLTECEFISVSKAYSLKKQVLAYFNDCGIEIDRYSPRFTEMERRLLLLNVSYRL
                     GGFNSWELPESFFERADRFIESVTENSGRFYDKENKEILSIGFAISFLRQQVCAVTID
                     SKFIEEIKKRPIYNYVESAWENTDFQTYYKKEEFAFILTLFNLCNYGFHSYQLIAEDF
                     QQLHQVFIDNTPEIKELVATFESHFNQELFGNQPFERALIHLMRSAWDNYQLFMPEKF
                     YLLNEEQTNLYKEVQTIFSSWSSQLPYDLRLNPNCMRAFVIELSGILRLTKEHLTIYI
                     VTNSDVHYLIYREALEAVTTFDFQVAPTIYSSISDIKKYAQQSSNRVLCERTLYTPDA
                     VQYENIIPISINTIDRAIISAVQNK"
     gene            complement(1655..2020)
                     /locus_tag="ACG842_001743"
     CDS             complement(1655..2020)
                     /locus_tag="ACG842_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HFQ0248284.1"
                     /translation="MTTELMQFLLPYFEKLVLGLDIFAICLLVWGVCLSIKDFLKYLF
                     VHTKVQSDRVSENNAIKKMLGGYILLSLEILVSAGIIESIIKPTLQDIFQLAALVVIR
                     TIISYFLNKEIGPVDVQNK"
     gene            complement(2017..2382)
                     /locus_tag="ACG842_001744"
     CDS             complement(2017..2382)
                     /locus_tag="ACG842_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HFQ0248285.1"
                     /translation="MFRSNLIEFLEWIIAILNICSIGILIVGIVLVLFKLFDWSHVKK
                     NYAERNTRNADTRKLLASYILLSLEVLVVADIIESVIKPTWTDILKLALIIIIRTVIS
                     YFLNQEIEDKEKQRRGEVG"
     gene            complement(2574..2822)
                     /locus_tag="ACG842_001745"
     CDS             complement(2574..2822)
                     /locus_tag="ACG842_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0248286.1"
                     /translation="MKNASLFSLSISKDSSFLLECQMIGREEKGNFDKKEEVSDFPVN
                     KNRVGKKKRFEEVYGSIFRNLGTKKAPRQCFFEKLMTD"
     gene            2821..2949
                     /locus_tag="ACG842_001746"
     CDS             2821..2949
                     /locus_tag="ACG842_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methanol dehydrogenase"
                     /protein_id="HFQ0248287.1"
                     /translation="MSKKLIKVGIGLGLLALGAAYLGKKTGLFEDDSHLYDEFESI"
     gene            3006..4700
                     /locus_tag="ACG842_001747"
     CDS             3006..4700
                     /locus_tag="ACG842_001747"
                     /EC_number="4.2.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050151 - oleate hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oleate hydratase"
                     /protein_id="HFQ0248288.1"
                     /translation="MRKSKAIMIGAGIANMAAAVYLIQEGKWKGDQITFYSLDDHGSN
                     DGAPTDTVVDEYWNKNHPLENQKGYIARGGRMLNYRTYVDLMDLLSRIPSVTEPGMTA
                     EEDTRDFDAKHRTFDKARLMEGGKGIIDGGKLGFNNKDRILLTKLIAMPDSEEEKLDN
                     ITIEEYFKDDPHFFETNFWFMWETTFAFRTRSSAQELRRYMHQMIYEFTQIEHLVGVN
                     RTRYNQYESIMLPLIEYLKEQGCKIILNRRVTDWEFKETPMQDEITVTGLKITNTLTQ
                     EEEYVVVDEDTAVIFTNGSITDSATLGDYETPAVENMDYGAASSLWKKASERFYNLGN
                     PDKFFADRDASEWVSFTLTTKNHLLLNEITRITTQEPGNALNSFISTTPITPLGQKDV
                     NMSIVVHHQPHFTSQKPNETVIWGYFLYPRRRGEFVDKQYIKMNGKEMLEELIGQLSK
                     VDPGPVNIREKETEIFDSVINNIPVYMPYASALFNNRAKSDRPKVIPKHSTNLAFTGE
                     FVEQPYQMIFTEQSAVRSGEIAAFHFAGIPMSRLVKTPRYDKDIPTLARAAKKMFE"
     gene            4800..4973
                     /locus_tag="ACG842_001748"
     CDS             4800..4973
                     /locus_tag="ACG842_001748"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0248289.1"
                     /translation="MQAFEHRLPRGYEKAALHQVIRAAKLKQLLMFLTWLDLLPQMQA
                     FEHRLPRGYEKGV"
     gene            5351..6484
                     /locus_tag="ACG842_001749"
     CDS             5351..6484
                     /locus_tag="ACG842_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HFQ0248290.1"
                     /translation="MKAVTWQGKQKMEIRNVDDPKILEPTDAIIRITATAICGSDLHL
                     YHHGEAVLEKGYVVGHEPMGIVEEVGSQVQTLKKGQRVVIPFNISCGHCHFCLNHMES
                     QCDESNPDQLYGGLFGFGKLNGNHWGGQAEYLRVPYADSTSFVVPDNDLPDEKVLFLS
                     DILPTAYWSVENAGVKKGDTVVILGSGPVGLFAQKFAVMAGAERVIAVDPVQHRLDKA
                     ANYNGVETYFLEDTAQAGTELYELAKGGADVIIDCVGMDGLEPVKEKAKNLVSLQSGT
                     ISPLQMASQAVKKFGTVQVTGVYMTPASSYPLQEFFMRDIAVKHGQAPIIHLMPKIYA
                     MIAEGLFDPTQIITHSLPLSEAAQAYEIFDKKEDKNIKVVLKP"
     gene            6515..6835
                     /locus_tag="ACG842_001750"
     CDS             6515..6835
                     /locus_tag="ACG842_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YrhK family protein"
                     /protein_id="HFQ0248291.1"
                     /translation="MPKIKRNTHEIEIGKEEDIEIRGRRFRFYFQNRYTLISLAVDLL
                     TGIFYIFGSLASLTKIPDRYGTYFYLAGAIFLTIRPILRIVRNIFIYDEKKAMKRTSK
                     KKEA"
     gene            complement(6864..7328)
                     /locus_tag="ACG842_001751"
     CDS             complement(6864..7328)
                     /locus_tag="ACG842_001751"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0248292.1"
                     /translation="MVTYNWIKLREHPELVKQSANWFSSKWGVPAVVYEESIRTGIAN
                     KESIPQWYVVLDEKETIVGGAGVIENDFHNRPDLTPNLCALFVEPEKRKQGIAKRILA
                     EIRKEMHQLGYEKLYLITDHTTFYERYEWTFLTMVKEESGTTARLYQSDTIG"
     gene            complement(7460..8713)
                     /locus_tag="ACG842_001752"
     CDS             complement(7460..8713)
                     /locus_tag="ACG842_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFQ0248293.1"
                     /translation="MDKFVAFMEKHFIPVASKIGAQRHLVAIRDSFMVSMPLMILGAL
                     AVMINNLPIPGFQELMNSIFGGESWKGFGAAAWNGTFAILSVLIAFLLAYHLANGYRK
                     DGVSAGVISLGSFFALGGALGMSSNGLFIAIIVGIISTEIFVRLSGNEKLIIKMPDGV
                     PPAVAKAFASLLPAMITISAFALVAAIFAAFGVDDIVGSFYTVVQEPFMGLANSYPSA
                     LLLAFITPFLWFFGLHGANMVDPLMQTINAPAIEANVNAIAAGHSAPFIVNKPFFDSF
                     VNLGGTGATLGLLLAIYLVGRKNKPYMVVTNLSIAPGVFNINEPTMFGLPIVLNPIMF
                     IPFILTPMVLVSVAYFATSTGLVPAATVMPPWVTPPIIGGVLATKSIAGGVLAAVNLV
                     LSILIYLPFVKVATIQERRKEALDA"
     gene            complement(8742..9059)
                     /locus_tag="ACG842_001753"
     CDS             complement(8742..9059)
                     /locus_tag="ACG842_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HFQ0248294.1"
                     /translation="MEDQKNLEAIMGLIMFGGNAKSDAMEAIAAAKKGDFTLADEKIK
                     DAEESLVQAHHSQTEMLTQEAQGNHVEVTLLTVHSQDHLMTSIAFTDLAKEIIDLYRR
                     LNA"
     gene            complement(9085..9405)
                     /locus_tag="ACG842_001754"
     CDS             complement(9085..9405)
                     /locus_tag="ACG842_001754"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFQ0248295.1"
                     /translation="MEKKTIMLVCSAGMSTSLLVTKMQKAAEAKGMEADIFAVSASDA
                     DNQLESKNVDVLLLGPQVRFMQNQFAEKLAPKGIPLDVINMADYGMMNGEKVLDQAEK
                     LINK"
     gene            complement(9869..11284)
                     /locus_tag="ACG842_001755"
     CDS             complement(9869..11284)
                     /locus_tag="ACG842_001755"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFQ0248296.1"
                     /translation="MKITIPKNFILGAASSAWQTEGWKGKKEGQDSFIDSWYKNEHFV
                     WHNGYGPAVATNFMEQYQQDVDLMKSIHLTHYRTSINWARFFTDYENCIVDEEYAQHI
                     SEMIDSLIEADVEPMICLEHYEVPAFLLEKYDGWSSRKVVDFYAKYAQIAFDRYGDRV
                     KNWFTFNEPIVPQTRCYLDAVRWPHEQNTKKWMMWNYHKVLATAKAVEIYRSGNYGGR
                     IGCILNPEMVYARSNSPEDQKAAYMYDLFYNRVFFDPMVKGYYTEEFLELCQKQSIYF
                     DPATEDLETIQHHTVDFLGINQYYPKRVRAPRYQWNEETPFHPERYYEVFDLPGKKMN
                     NSRGWEIYPKIMYDIAMYLKENYHNIPWLITENGMGREYEEQYMDEKGIVQDDYRIEF
                     IGQHLHYLIKATEEGANCEGYMLWAFTDCVSPMNAFKNRYGLVRIELNDERSRTLKKS
                     ASWYQTLIDKRELDLPDEIYK"
     gene            complement(11301..12617)
                     /locus_tag="ACG842_001756"
     CDS             complement(11301..12617)
                     /locus_tag="ACG842_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFQ0248297.1"
                     /translation="MRGSGMSFMDKLANVLGNVATRINNLRYIMVIKNAFAALIPVII
                     TGAFGTLFSAMVFDSENGLAQISFLRFLEELKPIASAISYVTLSFLTIYAVFLIGIEL
                     AKLNKVDGVFPGIIAVMSYLSVNPTVYELVNENQTVIVENVLAKQYTDTKGLFLGMLV
                     SILSIELYCWLRKQKKLQLRMPDTVPTNVSASFSALFPTILTVSGIAAAGFIIKELTG
                     MYLYDIIYNVVQRPLEGVVQGLPGILLLMFIAQVFWVIGIHGNQMVKPIREPLLLAAI
                     AVNTEAFEAGKEVPNIITMPFWDMYMSMGGSGVTIGLLIAVLLVGKREDMKQITKLSL
                     APGIFNINEPVIFGMPIMLNPILAIPFILTPLITGTIGYFATALGFAAKAVVMVPWPM
                     PPIVNAYLATAGNIGAVITQLVCIIVSILIYLPFVKVANRVENDGI"
     gene            complement(12767..13465)
                     /locus_tag="ACG842_001757"
     CDS             complement(12767..13465)
                     /locus_tag="ACG842_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HFQ0248298.1"
                     /translation="MEENKTLVEVTTDKILRYLKEMQLQVGDKLPNEYELSDYLEVGR
                     STVREAVRALASRNILEVRQGAGTFVSDKRGIANDPLGFSLIEDQDRMIRDLFELRYL
                     LEPKMAGLAAEYATKNQIVQMKKLTQKIETSFREGTQEHVQLDIDFHTMIAEASGNVA
                     LLHIIPIINESIALFNKNYDIQHLKIETIDIHTEIVQAIERRNALAAFDAMTVHMAIN
                     RRELQKIIGEKETN"
     gene            complement(13754..14743)
                     /locus_tag="ACG842_001758"
     CDS             complement(13754..14743)
                     /locus_tag="ACG842_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFQ0248299.1"
                     /translation="MYIADSARYEKMEYRKSGHSGLKLPILSLGLWQNFGDYDPIHNQ
                     REILRGAFDMGITHFDLANNYGGPAGAAEKNFGRIFREDFQAYRDELIISSKAGYHMW
                     EGPYGEWGSRKSIISSCDQSLQRMGLDYVDIFYHHRPDPDTPLEETAEALMQLVRQGK
                     ALYIGISNYNGEDTKKMTEILKRKEAPFIIHQMRYNMFSRALLEDDLSPVLEEEGLGA
                     ITFSPLAQGLLTNRYLHGIPEDSRAHRKEIPFLSEEQVGSTLEKIKALQTIAVSRGQS
                     LAQMALAWNLRQKSVTSVLVGASRLSQLQESVRMMDNLDFSPEEELRIDQILE"
     gene            complement(14823..15656)
                     /gene="msrA"
                     /locus_tag="ACG842_001759"
     CDS             complement(14823..15656)
                     /gene="msrA"
                     /locus_tag="ACG842_001759"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HFQ0248300.1"
                     /translation="MDEEQIFIQTLYNLILNPNTRDWERKVLIQTKNDIRENISVKEQ
                     LSKLEATLRPLAIRMNLTPDVMDFYLLITEGFDKEQKYDFSKHAMQDADYQERAVFAG
                     GCFWCMVEPFESKKGILSVLSGYTGGHVEKPNYDQVSGGYTGHVEAVEIIYDTREISY
                     SELLTIYWQITDPTDTFGQFQDRGKQYRPVIFYQVERQKELAEQSKQKLDSSGTFHQP
                     IVTKIEPAGTFWPAENYHQQFYKKQPKRYKKIQQARNQFLIYQRVKNKWQKNIRKNHF
                     D"
     gene            complement(15831..17465)
                     /locus_tag="ACG842_001760"
     CDS             complement(15831..17465)
                     /locus_tag="ACG842_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-pyruvoyl-tetrahydropterin synthase-related
                     protein"
                     /protein_id="HFQ0248301.1"
                     /translation="MKINKKVVILSGLIIVSSIYSFIFFKNTQSIYTTGDLSFHLSRI
                     KGLSTIFTSPINYETFNYTGYGVNYFYPFLTFFPAVMLYWMTKNLIVSYIIYVWLLNL
                     CTTLVAYHYGERFLKQKKAAFLFSCLYIFSAYRTVDIYYRSAIAEAIAITLVIPVLFY
                     AYQIISGKEEKYPSVKLALSMSLLVYSHVLSTLMSTALIIIFIFIRLVSKGFKNADFI
                     AIFKKLFSAAGMTLVLTSAFWYPMFEQMLYQKINKPSVTNLYAHASNVFDSLTEAMNN
                     DLTTYSMGLVGLLSLCIPLILFKKLTNIEKKIYYGTCLTWLATTSLVPWYLLQNTPAK
                     LLQFPWRILSLQIIFSSLILTMIFFKNRRYNKTRELFYLFMTVLLLITLTVSSKKNFN
                     QKIISQPGHIVVNKETVEAYTTKGMDYFLYDYSPIASLENKEHIIKHEAKIGSQWVNL
                     PYKTTGSSIIYRVTNEKSQVITLPVYAYLGTNVEINHQPVKEQVNANGLVQISGVKGV
                     ADIKVSTSYTVTTIISYLVTLTAYLCLFFKIVFHKR"
     gene            complement(17452..>19190)
                     /locus_tag="ACG842_001761"
     CDS             complement(17452..>19190)
                     /locus_tag="ACG842_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0248302.1"
                     /translation="NSSILKIELKKENNKSMKISKKAFLLVVLILLSTLYSVNFMRNA
                     QEIYTTGDLSFHLSRIKGLSSIFEGPINYTTFNNYGDGLNYFYPFLTIIPAVVFYGIS
                     NNLILSYVLYIWLLNICTILISFYYGEKFFKKIDAAFLFSCLYTFATYRTVDIYYRSA
                     IAEAIALTIIPAVLYYAYGLIFNREKKVIALSVSISLLVYTHVLTTLLCSALIACMII
                     VRLFLSKQIKNDIKFVVVNLVKAVFLTCALTSYFWYPMLQQMNYQEIHSPFKTDLQLE
                     ALSISDSIIQALNNDITTYTMGLLGLVALIFPLFILKEMEKKEKIIYVGVVSTWVCVT
                     NLFPWFLFQKTPISLLQFPWRILGIQIFFSSMIITLVFMKKTMNKKYSWLIIGATVVF
                     LLVTSIAAKENYAKVIQSKHGHIVINEETLPRYTTSDMGGLYDYAPTEAIKERPHLEK
                     HEVKVGESWEKGKYKAADNNITYTVASNKKQKVTVPVYAYLGTQVKVNGKVVNDSYKK
                     HGLVNVDVNAGENKIEITTKYTPTALMALWISILTYCLVVYSSFKRFAPNKLKDSRYV
                     TKKEINKKNENK"
BASE COUNT         6098 a         3755 c         3189 g         6148 t
ORIGIN      
        1 caaattttat aacataaaaa acctttttta acggcattta tatgcttttt cagaaattaa
       61 ctgatgattt caaaaagaaa acatgtcata atatttccca tattgtgcaa aaaaatacaa
      121 ggcggaggct ctactatgat tgaagaatat attgagaaaa atattttaag acagctgttt
      181 ttatgtgggc aattctatgt aaataaagaa gtcaatctag aaaagctctc caatctcctg
      241 catgtttgca aaaccacttt actcaatgat atcaataaca taaaaaaaga atttgaagaa
      301 cagatcgctt atacacatcg ggaaaaggac cgttacactt tatatttttc agaacatata
      361 cctcgatgca agatcatgca gcaattgtct caaaattcgt tgtttttaaa aacttgtctg
      421 ctttatttgg aagaagatga acctgattat cttcaattaa ctgaatgtga attcatctct
      481 gtcagcaaag cttattcatt aaaaaaacaa gtattagctt attttaatga ctgcgggatc
      541 gaaatcgacc gctactcccc tcgattcact gaaatggaaa gacgtctgct gcttcttaat
      601 gtgtcgtatc gtttaggcgg ttttaattct tgggagctac ctgaatcgtt ttttgaacgt
      661 gccgatcgtt tcattgaatc cgtaacggaa aatagtggca gattttatga taaagaaaat
      721 aaagaaatct tatcgattgg ttttgctatc agtttcctac gccagcaagt gtgtgctgtg
      781 acaatagatt caaaatttat cgaagaaatc aaaaaacgtc ctatttacaa ttatgtcgaa
      841 tcagcatggg aaaacactga tttccaaacc tattataaaa aagaagaatt cgcctttatt
      901 ttgacactat tcaatctttg taattatggt ttccattctt atcagttgat tgcggaagac
      961 tttcaacagc tacaccaagt gtttattgac aatactcccg aaataaaaga actagttgct
     1021 acctttgaat ctcacttcaa tcaagaatta ttcggaaacc aaccgttcga acgtgcgctg
     1081 attcatctga tgcgttcggc ttgggataac tatcaattat tcatgccaga aaaattttat
     1141 cttttaaatg aggaacagac aaatttatat aaagaagtcc agacaatttt ttccagctgg
     1201 tccagccagt taccttatga tctacggttg aatcctaatt gcatgcgggc ttttgtgatc
     1261 gaattgagcg gaattctccg tctgacaaaa gaacatctga ctatctatat cgtcacaaat
     1321 tcggatgttc attatctgat ttatcgggaa gccttggaag ctgtaactac tttcgatttt
     1381 caagttgcac caacaattta ttcaagtata tctgatataa agaagtatgc gcagcagtcc
     1441 tcaaaccggg tattatgtga acgtacatta tatacaccag atgcggtcca atatgaaaat
     1501 attattccta tttcgatcaa tacgatcgat cgagctatca tttcagctgt gcaaaacaaa
     1561 taaggggctg tgacaaaaat catgtcacag cctcttattt gttgaagatg cccatttctg
     1621 taacaatctc ttgatacacg tcaggctcac aacctcactt attttggaca tctacgggtc
     1681 cgatctcttt atttaaaaaa taagaaatga tggttcggat aactaccaac gccgctagtt
     1741 ggaaaatatc ctgcaaggta ggtttgataa tcgattcgat gatcccagct gaaacaagaa
     1801 tttccaaact tagaagtata tagcctccca acatcttttt aatagcattg ttctcgctga
     1861 ctcggtcgct ttgcactttt gtatggacaa ataaatattt caaaaaatcc ttgattgata
     1921 gacaaacacc ccaaaccaat aaacagatag caaagatatc aagtcccagt acaagttttt
     1981 cgaaatatgg aagaagaaac tgcatcaact ctgtcgtcat cctacctctc ctcttctttg
     2041 tttttctttg tcttctattt cctgattcaa gaaataagaa atgactgttc gaatgataat
     2101 aataagtgca agcttcaaaa tatctgtcca agtcggcttg ataactgact cgatgatatc
     2161 agcaacgact agtacttcca agcttaacaa aatataactt gctagcagtt ttcttgtatc
     2221 tgcattacga gtattacgtt ctgcatagtt tttcttgaca tgagaccaat caaaaagttt
     2281 gaatagaaca agaacgatcc cgacaattaa gataccgata gaacaaatat ttaaaatagc
     2341 aatgatccat tctagaaatt caattaaatt tgagcgaaac atatacgcta cctactttct
     2401 tttaatttgt tcctattctt ttattgtaaa agaaagttac cttgttcgtc tatttaaaag
     2461 ttgttctaat gataacggtt cgcttaaatt tttctttttc actctcccat ttttctccct
     2521 ccatccataa atcgtggaag tatccaaaac aacgtagcaa gttaaaataa ggcttagtcc
     2581 gtcatcagct tttcaaagaa gcactgtctc ggtgcttttt ttgtacccaa atttctaaaa
     2641 atagaaccat acacctcctc aaatcttttc ttcttaccta ccctattttt atttacagga
     2701 aaatcagaca cttcttcttt tttgtcaaaa tttccttttt cttctcgtcc tatcatttgg
     2761 cattctaata aaaacgaact atccttggat atagaaagag aaaacaagga ggcgtttttc
     2821 atgagtaaaa aattaatcaa agtcggcatc ggtttaggtt tgcttgccct tggcgctgca
     2881 tatctaggaa aaaagacagg actattcgaa gatgacagtc atttatatga tgaattcgaa
     2941 tcaatctagc atcctattta gttgaatcac tacggacata cttggaagac attggaggaa
     3001 tcacaatgag aaaaagtaaa gcaataatga ttggtgccgg tattgccaat atggcagcgg
     3061 ctgtttattt gattcaagaa ggcaaatgga aaggggatca gatcaccttt tattcattgg
     3121 atgatcacgg ttcaaatgat ggtgcaccga ccgacactgt agtggatgaa tattggaata
     3181 aaaaccatcc attagaaaac caaaaaggct atattgctcg aggcggtcgc atgcttaatt
     3241 accgtaccta tgtagatttg atggatctgt taagtcgaat accttctgtt actgagccag
     3301 ggatgactgc tgaagaagat acgagagatt ttgatgcaaa acaccgaacc tttgacaaag
     3361 cacgtttgat ggaaggtggc aaaggaatca ttgatggcgg aaaactggga ttcaataata
     3421 aagaccgtat tcttctgact aaactgattg ctatgcctga ttcagaagaa gaaaaactag
     3481 acaatatcac tatcgaagaa tatttcaaag atgatccaca cttttttgaa acaaacttct
     3541 ggtttatgtg ggaaacaaca tttgctttca gaacacgtag ttctgctcaa gaattgcgtc
     3601 gctatatgca tcaaatgatt tatgaattca ctcaaatcga acatctagtg ggcgtcaatc
     3661 gaacacgtta caatcaatac gaaagtatta tgcttccttt gatcgagtac ttgaaagaac
     3721 aaggatgcaa aatcattttg aaccgccgtg tcactgactg ggaattcaaa gaaacaccga
     3781 tgcaagatga aatcactgtg actggtttga aaattaccaa tacactgact caagaagaag
     3841 aatacgttgt ggtcgatgaa gatacagcag ttattttcac taatgggtcc attacagatt
     3901 cagctacttt aggagattac gaaacaccag ctgtagaaaa tatggattat ggtgcagctt
     3961 ctagtctctg gaaaaaagcc agtgaacgtt tctacaactt gggaaatcct gacaaattct
     4021 ttgctgatcg agatgcaagc gaatgggtca gctttaccct gacgacgaaa aaccatttgt
     4081 tgttgaatga aatcacccgt atcacgactc aagaaccagg aaatgccttg aattcattta
     4141 tttcaaccac accgatcacg ccgttaggcc aaaaagatgt caatatgtct attgtcgtgc
     4201 atcatcaacc gcattttacg tctcagaaac caaatgaaac agttatttgg ggttatttcc
     4261 tttatccaag acgacgtggc gaatttgtgg ataaacagta tattaaaatg aatggaaaag
     4321 aaatgctaga agaactgatt ggacaacttt caaaagtaga tcctggacca gtgaatattc
     4381 gtgaaaaaga aacagaaatt ttcgacagcg taataaataa tatccctgtc tatatgcctt
     4441 atgcttccgc gttgttcaat aatcgtgcca aaagcgatcg accaaaagtc attcctaaac
     4501 attcgaccaa tctggccttt acaggagaat ttgtcgagca gccttatcag atgatcttta
     4561 ctgaacaaag tgctgtacgt tctggagaaa tcgctgcttt ccattttgcg ggtattccta
     4621 tgtcccgtct tgtcaaaaca ccgcgttacg acaaagatat tcctactcta gctcgtgccg
     4681 caaaaaaaat gtttgaataa tcaagaggtt gtgaaaaagc tgccctgcat caagtaataa
     4741 gaacaaccag gctaaaacag cttcttatgt ttttgatttg gttggactta ttaccgcaga
     4801 tgcaagcttt tgaacaccgt ttaccaagag gttatgaaaa agctgccctg catcaagtaa
     4861 taagagcagc caagctaaaa cagcttctta tgtttttgac ttggttagac ttattaccgc
     4921 agatgcaagc ttttgaacac cgtttaccaa gaggttatga aaaaggcgtt tagtttcgag
     4981 tatgaagaaa aattttgtga aaatggttcc tcacgttttt gcataaattt gacttaatac
     5041 cgagaaactg atcccttctg tcataacctc tttttattta tgtctgtttc tttttgaaaa
     5101 caactatttt ttcaacgttt actctcttgc caatccaaca tcatctaacg gatacaacta
     5161 tttcaatgat taccattaca ctaaaaaatc ggaaacacac ctctccgaac acaaatacat
     5221 aagactatag aatgtttcat ttaagaaaaa aagcgtttca ttatcaaaaa ggtaagatct
     5281 cctttaaaat gaaattatct gtagtactaa taatgcagac aattttatga atctgaaagg
     5341 agcatctcca atgaaagcag tcacttggca aggaaaacag aaaatggaaa tcagaaacgt
     5401 agatgatcca aaaatattag aaccaacaga tgcaatcatc cgtatcacag caacagccat
     5461 ttgcggctcc gatctgcatc tttaccatca tggtgaagca gttttggaaa aagggtatgt
     5521 tgtaggacac gaaccaatgg gaatcgttga agaagtggga tctcaagttc aaacattgaa
     5581 aaaagggcaa cgtgtggtta tcccttttaa tattagttgt ggtcattgtc atttctgcct
     5641 gaatcacatg gaaagccaat gtgatgaatc aaatccagat cagttatatg gcgggctttt
     5701 cggatttggc aagttgaacg gtaatcattg gggtggtcaa gcggaatatc taagagttcc
     5761 ttatgcagac tctacttcat ttgttgtacc agataatgat cttcctgatg aaaaagtttt
     5821 atttctatca gatattttgc ccacagctta ttggagcgtt gaaaacgcag gagtaaaaaa
     5881 aggcgataca gttgtgattc taggttctgg tccagtcggt ctattcgctc aaaaattcgc
     5941 tgttatggct ggagcagagc gcgtaatcgc agtagatcca gtacagcacc gtctagataa
     6001 agcggctaat tacaatgggg ttgaaactta tttcttagaa gacacggcac aagctggaac
     6061 agaattgtat gaactggcta aaggtggcgc agatgtcatt atcgattgtg taggaatgga
     6121 cggattagag cctgtcaaag aaaaagcgaa aaatttagta agcttacaaa gcggcacaat
     6181 ctctccatta cagatggcat cgcaagctgt caaaaaattt ggaacagtcc aagtaactgg
     6241 tgtttatatg actccagctt cttcttaccc gctgcaagaa ttttttatgc gggatatcgc
     6301 tgtcaagcat ggacaggcac caattatcca tctaatgcca aaaatttacg caatgattgc
     6361 agaaggacta ttcgatccta cacaaatcat tactcattcg ctgccactat cagaagcagc
     6421 acaagcatat gagatctttg acaaaaaaga agataaaaat ataaaagttg ttttgaaacc
     6481 ttgaattcag taaaaaatac aggtgatttt ctttatgccc aaaatcaaac gaaataccca
     6541 tgagatcgaa ataggaaaag aagaagatat cgaaatacga ggacgtcgct tccgcttcta
     6601 tttccaaaat cgttacacat tgatctcact ggctgtcgat ttattaaccg ggattttcta
     6661 tatttttgga agccttgctt ccttaactaa aatccccgat cgatatggca cctattttta
     6721 tttggctggt gctattttct tgacgattcg tcctattctc agaatcgtaa ggaacatttt
     6781 tatttacgac gaaaaaaaag caatgaagag aacaagcaaa aaaaaagagg catgagaatt
     6841 tttcaaatct catgcctctt ttttcaccct atcgtatcac tttgatacag acgagcggtc
     6901 gtcccacttt cttccttgac cattgtcaaa aatgtccact cgtatcgttc ataaaacgtg
     6961 gtatgatctg taattaagta aagtttttcg tacccaagtt gatgcatttc ttttctgatt
     7021 tccgctaata tccgcttggc tatcccctgc ttacgctttt ccggttcaac aaacagggca
     7081 caaagatttg gtgtcagatc agggcggtta tggaagtcgt tttcaatgac gcccgctcct
     7141 ccgacgatgg tttctttttc atctaagacc acataccatt gaggaatact ttccttatta
     7201 gcgattccag tacgaatact ttcctcatac acaacagcag gaactcccca ttttgatgaa
     7261 aaccaattcg cgctttgttt tactaattct gggtgttctc ttaatttgat ccagttatat
     7321 gtaaccaatg tttaaacctc cgactaaaat aaaacgttta caaaaattat acaagtcttt
     7381 atgaaaatta gcaatgacaa caatagtgat gtaaaaaaaa aaacggaaaa caatcacttg
     7441 ttctccgttt tttgattaat caagcatcta aagcttcttt tcttctttct tggatcgtag
     7501 ctaccttgac gaacggtaga tagatcaaaa tagaaagaac caaattgaca gcggctagta
     7561 caccacctgc aatacttttt gttgccaata caccgccaat aattggtgga gttacccatg
     7621 gaggcataac ggtagctgct ggcactaaac ctgtagaagt cgcaaagtaa gcaacactaa
     7681 ctaagaccat tggtgtcaaa ataaatggaa tgaacatgat cgggttcaac acgataggta
     7741 acccaaacat cgttggttca ttaatattga agacacctgg cgcaatcgac aagtttgtta
     7801 caaccatata tggtttgttt ttacgtccta ctaaataaat agctaacagt aaacctaaag
     7861 ttgcacctgt tcctcctaag ttgacaaagc tgtcaaagaa aggtttgttc acgataaacg
     7921 gtgcgctatg tccagctgcg attgcattaa cgtttgcttc aatggctggc gcattgattg
     7981 tttgcatcaa aggatcgacc atattcgctc cgtgtaaacc aaagaaccat aagaatggtg
     8041 tgataaatgc aagcagtaag gcagatggat aagagtttgc aagtcccata aatggttctt
     8101 ggaccactgt atagaaagaa cctacgatat catcaacacc gaatgcagca aaaattgcag
     8161 caactaaagc aaacgcagag atagtgatca ttgctggtag taatgaagca aatgcttttg
     8221 caactgccgg tggcacacca tcaggcattt tgatgatcaa tttttcattt ccgcttaaac
     8281 gtacaaagat ttctgttgaa ataatcccaa caataatagc aataaacaaa ccgtttgagc
     8341 tcatacctaa tgctccgcct aaagcgaaga aagagcctaa agaaatcaca cctgcggaaa
     8401 caccgtcttt tcggtagccg ttagctaaat gataagcaag caagaaagca atcaagacag
     8461 aaagaatggc aaatgtaccg ttccaagcag cagccccgaa tcctttccat gattcaccac
     8521 caaaaattga gttcatcaat tcttggaacc cgggaatcgg taagttattg atcattactg
     8581 ctaatgctcc taagatcatt aacggcatac tcaccataaa tgagtcccga atcgcaacta
     8641 aatgccgttg tgcaccaatt tttgatgcta caggtataaa atgtttctcc ataaatgcaa
     8701 caaatttatc cataaatctt ccctcttttc tttactgtag attacgcgtt caatcgacga
     8761 tacagatcaa tgatctcttt tgctaaatct gtaaaagcta tagatgtcat cagatgatct
     8821 tgactgtgga ccgtcaaaag cgtgacttca acgtgatttc cttgtgcttc ttgtgtcagc
     8881 atctctgttt gagagtgatg tgcttggact aaagattcct ctgcatcttt gattttttca
     8941 tctgctaaag tgaaatcacc ttttttcgca gcagcaatcg cttccattgc atcgcttttt
     9001 gcatttccgc caaacatgat cagacccatg atggcttcta gatttttttg atcttccatt
     9061 tattgtatcc tccgataatt tttattattt gtttattagt ttttctgctt ggtcgagtac
     9121 tttttcacca ttcatcatgc catagtcagc catattgatt acatctaaag gaatgccttt
     9181 aggtgctagc ttttcagcaa attgattttg cataaagcgt acttgcggtc ctaaaagtaa
     9241 aacgtctaca tttttagatt ctagttgatt atccgcatct gaagcagata cagcaaagat
     9301 gtctgcttcc attccttttg cttctgctgc cttttgcatt tttgtgacta ggagacttgt
     9361 actcattcca gctgaacata caagcatgat tgttttcttt tccattttaa acacgttcct
     9421 ttctatactt acacttgtat ataagcaagt agcgtgccaa ctttaaaagc gctttaaaaa
     9481 gttgatatca tcacatttaa ggtctatacc aagttgcata cacactaaaa aagaaagcgc
     9541 acactaaaca cacaaattaa tttttattac cttttttctt ttaacgtgca caaaaaaagt
     9601 tgtgacacaa aacttgtcac aacttttttc aaataaacgg tattcaaaag cttgcatctg
     9661 cggtaataag tccaaccaag ttaaaaacat aagatgttgt ttttactttg ctattcttat
     9721 tacttgatgc aggacagctt tttcacaacc tctcaataaa cggtgttcaa tcagctgacc
     9781 ctgggtatta gctcaaaacg ctggattcac aacctctttt atttcaaata accagtaaat
     9841 acaatgaaca tctacaaaaa acttctattt atttatagat ttcgtcaggc aaatccaatt
     9901 ctctcttatc aatcaaagtc tgataccacg aagcagattt tttcaatgtt ctgcttcgtt
     9961 catcgttcaa ttcgattcga actaatccat atcgattctt aaatgcattc attggtgaca
    10021 cacagtcagt gaatgcccat aacatatagc cttcgcaatt tgcgccttct tctgttgctt
    10081 tgatcaagta atgcaaatgc tgtccgataa attcgatacg gtaatcatct tgcacgattc
    10141 ctttctcatc catatactgt tcttcatatt ctcttcccat accgttttct gttatcagcc
    10201 aagggatatt atgataattt tcttttagat acatggctat atcatacatg attttcggat
    10261 agatttccca tcccctggaa ttattcattt tcttcccagg taaatcaaag acttcataat
    10321 atcgttcagg atggaaaggt gtctcttcat tccattgata acgaggggcg cgaacgcgtt
    10381 taggataata ttgattgatt ccaaggaaat ctactgtatg atgctgaatc gtttctaaat
    10441 cctctgttgc aggatcgaaa taaatcgatt gtttttgaca caactccagg aactcttctg
    10501 tgtagtatcc ttttaccatc ggatcaaaaa atactcgatt ataaaacaaa tcatacatat
    10561 aagcagcttt ttggtcttct ggagaattgg atcgagcata gaccatttca ggatttaaaa
    10621 tacagccaat ccttccacca taatttccgc tgcggtaaat ctctactgct ttagccgttg
    10681 ctaaaacttt atgataattc cacatcatcc acttttttgt gttttgttca tgcggccagc
    10741 gaacagcatc caaatagcaa cgcgtttggg gaacgattgg ttcgttgaat gtaaaccagt
    10801 tttttaccct atcaccgtaa cgatcaaagg caatttgagc atacttagca taaaaatcaa
    10861 ctacttttct tgaagaccag ccatcatatt tttccaaaag aaaagctggg acttcataat
    10921 gttccaaaca aatcatcggt tctacgtctg cttcgatcaa agagtcgatc atttcactta
    10981 tatgctgagc atattcttca tctactatac agttctcata atcagtaaaa aaacgagccc
    11041 aattgataga agtccgataa tgtgtcagat ggatggattt catcagatcc acatcctgct
    11101 ggtattgttc cataaaatta gttgctacgg ctgggccata cccattatgc caaacaaaat
    11161 gttcattttt ataccagcta tctataaacg aatcctgtcc ttcttttttt cctttccaac
    11221 cttccgtttg ccaagcagaa gatgcagcac caagaataaa attttttgga atagtaattt
    11281 tcatgatttc ctcctgtttt ttagatccca tcattttcca cacgatttgc tacttttaca
    11341 aatggcagat agatcaaaat agaaacaata atacaaacaa gttgcgtaat aactgcaccg
    11401 atatttcctg ctgttgccag ataagcattg acgatcggcg gcattggcca agggaccatt
    11461 acaactgctt tcgccgcaaa tcctaaagcg gttgcaaaat aaccaatcgt tccagtgata
    11521 agaggcgtta agataaacgg aatagctaaa attggattca gcatgatcgg cataccaaaa
    11581 ataactggtt cattgatgtt gaagatccca ggagcaaggg ataacttggt gatttgtttc
    11641 atatcttccc gttttccaac taacaatact gcaatcagta aaccgatcgt gactccagac
    11701 ccgcccatac tcatatacat atcccaaaat ggcatagtaa taatgttcgg tacttctttt
    11761 ccagcttcaa aagcttccgt atttacagca attgccgcta aaagcaacgg ttcccgtatc
    11821 ggcttcacca tttgatttcc atggatacca atcacccaaa agacttgcgc aataaacatc
    11881 aatagcaaaa tacccggtaa cccttgtacg acaccttcta atgggcgttg tacgacattg
    11941 taaatgatat cataaaggta cattcctgta agttctttga tgataaatcc cgcagccgcg
    12001 atacccgata cagtcagaat cgtcgggaat aaagcagaaa aactagcaga aacattcgtt
    12061 ggcacggtat caggcattct cagctgtagt ttcttctgtt tcctcaacca gcaataaagt
    12121 tcaatagaaa ggatggagac caacattccc aaaaataacc ctttcgtatc tgtatattgt
    12181 ttagccaata cgttttctac tataacagtc tgattttcat tcactaattc ataaacagtt
    12241 ggattaacag ataaataaga catcactgcg atgataccag ggaatacacc atccacttta
    12301 ttcagcttgg ctaattcgat ccctatcaga aaaacagcat agatggtcaa gaaacttagt
    12361 gtgacatagc tgatggcaga tgcgataggc tttaattctt ctaaaaaacg caagaaactt
    12421 atctgtgcta agccattttc cgagtcaaag accattgctg aaaacaatgt gccaaaagca
    12481 cccgtaataa ttaccggaat caatgcagca aatgcgtttt ttatcaccat gatatacctt
    12541 aaattattga ttctcgttgc cacgttccct aaaacattgg ctaatttatc cataaaactc
    12601 attccagatc ccctcattcc tgcttcttct ccttgacgtc atctcttttt gtctattagt
    12661 cagcgcttac atttcgatga acagaaacta tttactcctc ttttttacca aatataaaac
    12721 aaaaattcat ctgatgtatt tatcataaaa tacatcagat gaactgtcaa ttagtttctt
    12781 tttctcctat tattttctgt aattctctcc gatttattgc catatggaca gtcattgcat
    12841 caaacgcagc aagtgcattc cgcctttcga tggcctgaac gatctctgta tgaatatcga
    12901 tcgtctcgat tttcagatgc tggatatcat aatttttatt aaacaaagca attgattcat
    12961 taatgattgg gataatatgc agcaaggcta cgtttccgct agcttctgca atcatcgtat
    13021 gaaaatcgat gtctaattga acatgttctt gtgtgccttc tcgaaaagaa gtttcaatct
    13081 tttgcgttaa ttttttcatt tggacgattt gattcttcgt tgcatattcc gctgccaaac
    13141 ctgccatctt aggctccaat aaatatctca gttcaaataa gtctcgtatc atccgatcct
    13201 gatcttcaat caaagaaaaa ccgagtgggt cattggcaat ccctcgctta tcactgacaa
    13261 atgtccccgc tccttgccga acttcaagga tattcctgga agctagagcc cgcacagctt
    13321 ctcgcaccgt gcttcgcccc acctctaaat aatcggacaa ttcatattca ttcggcagtt
    13381 tatctcctac ttgcaactgc atttccttta aatatctaag gatcttgtca gtagttactt
    13441 ctactagggt tttattttct tccaaaccac atacctcctt ttcatctgat gtattttttc
    13501 gtaattatta taacacgttt gaatcatctg atgatttttt gtatacaaaa ttgattaatc
    13561 cgttcataga catacaaacg aagaagatat ttaccataaa aaagactagg ataaaattca
    13621 ctcctagcct tcgatcattt ttattttgaa cgacaaaaaa tatgatgttc aataacagac
    13681 gatcttcatt cgattcttga tgtcatcttt tgtttttttt aatatatgga taaaaagttt
    13741 gattacatcg tttctattct agaatttggt cgattctcaa ttcttcttct ggtgagaaat
    13801 ctaaattatc catcattcga acactttctt gtaactgact taaccgactt gctccgacca
    13861 gtacacttgt aacagatttt tgtcgcaaat tccatgcaag ggccatttgc gctaatgatt
    13921 gtcctcgtga tacggcgatt gtttgcagtg ctttgatttt ttccagagtt gatccaactt
    13981 gttcttctga aaggaatggt atttccttac gatgtgctct agaatcttca ggaataccat
    14041 gtagataacg atttgtcagc aacccttgag caagcggact gaacgtgatt gcccctagcc
    14101 cttcttcttc taatactgga gatagatcgt cttctagtag tgcacgagaa aacatattgt
    14161 agcgcatctg atgaataatg aacggtgctt cctttctttt taaaatttca gtcatttttt
    14221 ttgtatcttc tccattatag ttggatatac caatatataa tgcttttcct tgtcttacaa
    14281 gttgcatcaa agcttctgct gtttcttcca aaggtgtatc agggtctggg cgatgatgat
    14341 agaagatgtc aacataatct agccccattc tttggagact ttggtcacaa cttgaaatga
    14401 tggatttacg agagccccat tccccatatg gcccttccca catatgataa cctgctttgc
    14461 tagaaataat cagttcatca cggtaggctt gaaaatcttc tctaaaaata cgcccaaagt
    14521 ttttctctgc agctccagca gggcctccat aattattcgc aaggtcgaaa tgggtaattc
    14581 ccatgtcgaa tgcgcctctt aaaatctctc tttgattatg gataggatca taatcaccaa
    14641 aattttgcca taatccgaga gataaaatcg gtaatttcaa acctgaatga ccggatttac
    14701 gatattccat tttttcataa cgtgcactat ctgcaatata caatttttct cctcctcatc
    14761 aaatcttttt aaaacgcttt cttttttcta tttttagttt actacatttt aaagaaaagg
    14821 agttaatcaa aatgattttt tcgtatattt ttttgccatt tgttttttac tcgttgatag
    14881 atcagaaatt gattacgtgc ttgctggatt tttttataac gtttcggttg ttttttgtaa
    14941 aattgctgat gatagttttc tgctggccaa aacgtaccgg ctggttcgat ttttgtgacg
    15001 attggttggt ggaaagttcc tgaactgtct agtttctgtt ttgattgttc cgctaattct
    15061 ttttgtcgtt cgacttgata aaaaatcact ggacgatatt gcttccctct atcttgaaat
    15121 tgtccaaatg tatctgtggg atcagtaatt tgccaataga ttgtcagcaa ctcgctataa
    15181 cttatttctc ttgtgtcata aatgatctcc acagcttcca catgacctgt gtaaccacca
    15241 cttacttgat cataatttgg tttttcaaca tgtccgcccg tgtatcctga caaaacagat
    15301 aggatccctt ttttcgattc aaacggctcg accatacacc agaaacatcc ccctgcaaat
    15361 actgcacgtt cttgatagtc agcatcttgc atggcatgct tggaaaagtc atatttttgc
    15421 tccttatcaa atccttccgt aatcaataaa taaaagtcca ttacatcagg cgtcagattc
    15481 attcggatag ctaatggacg tagtgttgct tctagttttg ataactgttc tttaacggat
    15541 atattttctc ttatgtcatt ctttgtctga atcaatactt ttcgttccca atctcgtgta
    15601 ttcggattca aaatcagatt ataaagtgtt tgaatgaata tttgttcctc atccatagat
    15661 tctcctcctt tccgaagaag acaagctagt gactttagct gtaatttatc ataaagtgag
    15721 gaaataagcg attacttcat ccccctacag atttttcgta taaaaaagag agaatcctta
    15781 tcgacagatt ctccttttta atcaatttat tttataaatg aaaagttaat ttacctttta
    15841 tggaaaacta ttttaaaaaa taaacataaa tatgcagtta atgtaacaag ataagaaata
    15901 atagtcgtta ctgtataact tgtgcttact ttaatatcag caactccttt gactccgctt
    15961 atttgtacca atccattagc attcacctgc tcctttacag gttgatgatt tatttctaca
    16021 ttcgttccta aataagcata tacaggaagt gtgattactt ggctcttctc attagttact
    16081 ctatatatga tagaactacc agttgtttta taaggaaggt ttacccattg actgccaatc
    16141 tttgcttcgt gttttattat atgctcctta ttctccaatg aagcgatagg agaataatca
    16201 tataaaaaat aatccattcc tttagtggta tacgcctcta ctgtttcttt gtttacaaca
    16261 atatgtcccg gctgagaaat tattttttga ttgaaatttt ttttactaga aacagttaaa
    16321 gtgattaata acaaaacagt cataaataaa taaaatagtt ctcttgtctt attgtatctt
    16381 ctatttttga aaaatatcat cgttaatatc agagaagaga atattatttg caagcttaag
    16441 atccgccaag gaaattgtaa caatttggct ggcgtatttt gcagaagata ccatggcacc
    16501 aaactagttg tagctagcca agtcaaacac gtaccataat aaatcttttt ttctatgttt
    16561 gttaattttt taaataaaat caggggaata cataaagata acagaccaac tagtcccatt
    16621 gagtaagtag ttaagtcgtt gttcatagct tcagtcagac tatcgaacac attagatgca
    16681 tgagcatata aatttgtcac gctaggtttg ttgatttttt ggtagagcat ttgttcaaac
    16741 atcggatacc aaaaagcact cgtcagaacc aatgtcattc ctgccgcact aaataatttt
    16801 ttaaatatag caataaaatc tgcattcttg aaacctttag aaaccaaacg aataaaaata
    16861 aaaatgataa ttaatgctgt actcattaat gttgatagta catggctgta cactagcaaa
    16921 ctcatactca atgctagctt tacagacgga tacttttctt cttttccaga gatgatttga
    16981 taagcataaa ataatacagg gataacaagt gttattgcga tagcttcggc aattgcggaa
    17041 cgatagtaga tgtctactgt ccgataagca gagaaaatat agagacaact aaataaaaat
    17101 gccgcttttt tttgttttaa aaaccgctcg ccataatgat aagctacgag tgttgtacac
    17161 aagttcaata accaaacgta aataatatag cttacaatca aattttttgt catccaatat
    17221 aacattacag ctggaaaaaa agtcagaaag ggataaaaat aatttacacc atatccagta
    17281 taattgaatg tctcataatt aatcggactg gtaaaaatag tcgataaccc cttgattcga
    17341 gataagtgaa aagaaagatc tccagtggta taaatactct gtgtattttt gaaaaagatg
    17401 aaagaatata tactactaac tataattaat ccactcaata ttactacttt tttatttatt
    17461 ttcattcttc ttgtttatct cctttttagt cacatatcga gaatctttca acttattagg
    17521 tgcaaatcgt ttaaaacttg aatacaccac taaacaataa gtcagaatac tgatccataa
    17581 agccatgaga gcagttggag tatattttgt tgtaatttct atcttatttt ctccagcatt
    17641 tacatccaca ttcactaagc catgtttttt ataagagtca tttacgactt taccgtttac
    17701 ttttacctgt gtacctaaat aagcatatac tggtactgtc actttttgct ttttgttgct
    17761 tgccactgtg taagtaatgt tattatctgc agctttatac ttcccttttt cccacgattc
    17821 tcctactttt acttcatgtt tttctaagtg aggcctttct tttatcgctt ctgttggggc
    17881 gtaatcgtat aatcctccca tatctgatgt tgtataacgt gggagtgttt cttcattgat
    17941 aacaatatga ccgtgttttg attggataac ttttgcgtaa ttttcttttg ctgctataga
    18001 cgtgacgagt agaaacacca cagtagcgcc aatgattaac catgaatatt ttttattcat
    18061 agtctttttc ataaaaacaa gcgttatgat cattgaagaa aaaaagattt ggatacctaa
    18121 gatacgccaa ggaaactgga gtaagcttat cggtgttttt tgaaacaaaa accatggaaa
    18181 taaattagtt acgcataccc atgtagaaac tacgccaaca tagatgattt tttctttttt
    18241 ctccatttct ttcagtatga ataaaggaaa aatcaacgca accaatccta ataaccccat
    18301 agtgtatgtt gtaatatcgt tatttaacgc ttgaatgata ctatctgaaa tagaaagtgc
    18361 ttctagctgc aaatctgttt tgaagggact gtgtatttct tgataattca tctgctgcaa
    18421 cataggatac caaaaatagc tagttaatgc acaagtcaaa aaaacagcct ttactaaatt
    18481 cacgaccaca aattttatat cgttttttat ttgcttgctt aaaaacaaac gaactataat
    18541 catacaagca atcaatgcac tacacaatag tgtagttaag acatgagtat acacaagtag
    18601 acttatactg acagataaag cgatgacttt tttttctcta ttgaatatca atccataagc
    18661 ataatataaa acagctggga taatcgtaag agcgattgct tctgcaattg cggatcgata
    18721 atagatatca actgttcgat aagttgcaaa agtatataaa caactaaata aaaaagcagc
    18781 atctatcttt ttaaaaaact tttctccata ataaaaacta atcagaatcg tacaaatatt
    18841 taataaccaa atatatagaa cataacttaa tattaagtta tttgaaattc cataaaaaac
    18901 gactgcagga attattgtaa gaaaaggata aaaatagttc agtccatctc cataattgtt
    18961 aaatgttgta tagttgattg gcccttcaaa aatagaagat aaccctttga ttcgtgaaag
    19021 atgaaaagat aaatcacctg ttgtataaat ttcttgagca tttctcataa aatttactga
    19081 atataaagta ctaagtaaga taagaactac taaaagaaat gcttttttac ttattttcat
    19141 agatttgttg ttctcctttt ttaattctat tttcagtata ctagaattgt
//
LOCUS       DAZKLM010000055        18657 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3764
            SAMEA115706913-rid22582563.denovo.056, whole genome shotgun
            sequence.
ACCESSION   DAZKLM010000055 DAZKLM010000000
VERSION     DAZKLM010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706913
            Sequence Read Archive: ERR13196647
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18657)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18657)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287823.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287823.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,004
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,849
            CDSs (with protein)               :: 2,849
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 41 of 96
            Pseudo Genes (incomplete)         :: 52 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 15 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18657
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706913-rid22582563.denovo.056"
                     /isolate="VRE3764"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(43..954)
                     /locus_tag="ACG842_001762"
     CDS             complement(43..954)
                     /locus_tag="ACG842_001762"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFQ0248303.1"
                     /translation="MKKRFLALAIVLGTGLLSGCTNAGEKTAVSYKGGTISEQEVMDS
                     LKKMQGADSAVQQLIVYQVFEDKYGDDVSTKEIDSQYDQTKKQLGDSFDSQLKSAGYT
                     EQTFKDSIKQSLAFQEGLKKHIKLTDEDLKTAWESFHPEVEAQIIQVASEDDAKDVKK
                     AADKGDDFSKLAKDKSTDTTTKEDGGKVKFDSTTTTVPAEVKEAAFKLKDGQVSDVIT
                     STNASTYTTEYYVVKMVKNQNKGNDMDKYKKELKEIATDTKLSDSTFQNKVIGEVLKD
                     ANVKIKDKDFENVLSTFTSDSSTASSK"
     gene            complement(1331..1969)
                     /locus_tag="ACG842_001763"
     CDS             complement(1331..1969)
                     /locus_tag="ACG842_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0248304.1"
                     /translation="MEPLIVIKGAKKIIRNKTILHLDRFTADAGDFVTVVGESGAGKS
                     TLLNILGLNDQFSSGQFYLFNQETKHLTGREKRLIKRNALSFLFQDFGLIEYETIDFN
                     LDIGLKFSNLNVSDKRTAKYEALKKVNINKKLSTPIYTLSGGEKQRIACARVLLKNSK
                     IILADEPTGSLDKRNKEQVVEILSQQASQGTLVIVVTHDPYLTKIGNKKIML"
     gene            complement(1973..3955)
                     /locus_tag="ACG842_001764"
                     /pseudo
     CDS             complement(1973..3955)
                     /locus_tag="ACG842_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015370994.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1430 domain-containing protein"
     gene            4135..4800
                     /locus_tag="ACG842_001765"
     CDS             4135..4800
                     /locus_tag="ACG842_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0248305.1"
                     /translation="MNIGEKIRTYRRNCKLTQKELGEKIGVSDKTISSWENSRTMPDL
                     EMLSLLHQTLGLPIDFPSIESATESIEPTLPDEPLVIKKSYSSKQPFSLKKTLSFFLL
                     VFCLVYLFLINYQYKNETVDRFNPFLKPQIGYTTLPVGYVENSIEHWVCNDIFVHGTW
                     LSFHAGEVEKGKNHAVIKHKGKHVMNVRLLTWEAIPSDYQEILIQDYDDALKRSPQEP
                     DRH"
     gene            complement(5197..7230)
                     /gene="brxL"
                     /locus_tag="ACG842_001766"
     CDS             complement(5197..7230)
                     /gene="brxL"
                     /locus_tag="ACG842_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease Lon-related BREX system protein BrxL"
                     /protein_id="HFQ0248306.1"
                     /translation="MEQQEILKLSEFFPGKIVRKDLTKKIKEGANVPVYVLEYLLGMY
                     ASSLDETLVEKGVENVKNILARNYVRPDEAEKVKAKIRELGGYTVIDKVTVHLNEKKD
                     CYEAEFSNLGLKNVQISEEYPKKFDRLLGGGIWCIVSMEYWYDENDRWNSPFIAKKVD
                     PIQMPGLDIEAYKMARKEFTTNEWIDLLLRSIGMEPTQFNKRVKWLHLARLIPLIENN
                     FNLCELGPRGTGKSHVYKEISPNSILVSGGQTTVANLFYNMASRTMGLVGMWDCVAFD
                     EVAGIKFKDNDGVQIMKDYMASGSFARGKEEKNASASMAFVGNINQSVDVILKTSNLF
                     EPFPEVMGTDSAFLDRMHCYLPGWEIPKYRPDFFTNDYGFITDYFSEVMRELRKVSFG
                     DSFDKYFKLGSQLNQRDTIAVRRIVSGMMKLLYPDGRFEKSDVEEILRFALEMRRRVK
                     EQLKKIGGMEFYDVNFSYIDLEEEREEYVSVPEQGGGTLIPEGLGKPGHLYTVSYGKT
                     GQLGTYKLETQMTSGNGKFERTGINSDAGAREAMDSAYRYLRANYGSVSQSISLTTKD
                     FVVHVQDMNGIGMTKTLSLSTIIALCSVSLNKPVLPSLVILGDITIGGSMIKVDNLAN
                     CLQVAKDAGAKKVLVPMISAADIGTVPPELMGTFNLIFYQTPEDAVFKALGVE"
     gene            complement(7348..9870)
                     /gene="pglZ"
                     /locus_tag="ACG842_001767"
     CDS             complement(7348..9870)
                     /gene="pglZ"
                     /locus_tag="ACG842_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BREX-1 system phosphatase PglZ type A"
                     /protein_id="HFQ0248307.1"
                     /translation="MTDINTQQIEEKLNQRFPNYGCRKIIFWFDSNQDFLEEIDSLVI
                     QNAKIYRLEKDAQFKTKRLLEYEDPETNYLIYAPFPRWDDKDEDNHLLSVLNYSEEFS
                     ADRLAIIMNELAIPLRFHQTVKQYRNFFNAKDRMRSFGEFSTHMTINKSETIELMIMA
                     VLLKSKSIRLNDLLRVVLKSFTESRENPLRELDKYQLTAKFWEFIQMKFGFTDSQPTL
                     EKLTMCLYLNYFYYQIDQEMPKKFKGFEILHLKANIITFMDQFMNDTRYMESFEQLSK
                     KVYQAIDGDGLLDSIEIDDLIEADVFSEIHAKILMFYAERLKASDTTTKIAGQTIFDS
                     LKQREKMHFASDYIYEYQLLKHAYYLVDKNRLCLPMNMDNLLTQYEKEMYWVDTHYRK
                     FIWNSDHIEEKQKYGQLYRLVEANYSKFLDQSGQIWNQTFEFKNRPSIRKFYNQAVMK
                     RSVKTVVIISDAFRYELGMNLERKLVREKKYSTNMASFLSVLPSVTEFGKAALLPNNQ
                     LTYNEGTEVLIDGKKTNGLSNRNAILQQRDANAVALNYDDVVKMNKAEVREVFNGKSV
                     IYIYHDQVDRTGDHSNENRVFEACDLAISEISRLINRLHNDGSIYRFIVTADHGFIYK
                     RSHVEEVDKIENPSRNEKDRVERRFILSEEEYEIIGTKNYSLGDSLGNDDTRNICVPI
                     TSSIFKKAGGGQSYVHGGSSIQEMLVPVLEITVAKGSNAKEPVAIELMTSNRRITGLS
                     TTLEFYQKNAISDVYDPASFWLYFEDEYGERISNEEIYVADSKTEDANQRFTKFVFEF
                     VNRRYTVEDTYYLVIKNQKNDLTERIKFIIDNPFAESFDFDI"
     gene            complement(9986..13618)
                     /gene="pglX"
                     /locus_tag="ACG842_001768"
     CDS             complement(9986..13618)
                     /gene="pglX"
                     /locus_tag="ACG842_001768"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BREX-1 system adenine-specific
                     DNA-methyltransferase PglX"
                     /protein_id="HFQ0248308.1"
                     /translation="MDKKAIKNFAVNARRKLIDEVMTKAKRLGIDESKIEDVKKIDSN
                     LQEIEKSGIRIQGKEIAQRNKLIAELKQRASVTSYSEAFQELIEEVAYTWFNRLIAIR
                     FMEVNDYLPDTYRVLSSEISGKIEPDIITDIYDADIFEELSSNEQQQVRTWKTDNSAE
                     AMDKLYQLIFIKICNQLNDYLPELFEEVEDYTELLFTASYIKKDGVIADLLSIPEDDF
                     YVQAGGQVEIIGWLYQYYNSEPHNQVVNIYKGIVKKQDIPAATQLFTTDWVVRYMVDN
                     SLGKYWLDHYPNSELRNKLKYLIPDKYAFEQATSVSNLKPEELKIIDNAMGSGHILVY
                     AFDVLMEIYLTLGYSERDAARAIVEYNLYGLEIDKRAYQLSYFAIMMKGRQYDRLFLK
                     KSLSPNLREFIDSPKVTDEYQERISELNNDKYSERWNEYKELIFDQFENGKELGSIIK
                     LVDKNNQAITKEYLGKVREYISKFNEFTNMDILYGMKEIQEKYLHILDVAEMLVDKYV
                     AVVTNPPYLNKMDKKLKKYVNDNYSDVKKDLFSVFIKLNSQMLIDNGYASFMTPFVWM
                     FISSYEKLRSFLINERNISSLIQMEYSAFEEATVPICTFTIKNSNEQDGSYIKLSDFK
                     GGMEVQKIKTLEAIHQHGECDYFYSTNQDNFKKIPGMPIAYWASDNVITDFAQGKLTN
                     DVADAKQGLATGNNNKFLRYWFEVEFNKIKFDAKTLEDAKKSRKKWFPTTKGGRFRKW
                     YGNNEYVVNWENDGDEIKNYVNENGKLKSRPQNLNYYFKKGITWSTLSSGQVSFRLQN
                     QAIFETKGSVLFSKDITDFEEIFGYVNSKLVNHFLTFISPTLDYHEGPMGKLPFLKVT
                     SERKLKKIREKVDENIDIARKDYNALETSYEFRRNILLSYMFKKDLSEAFNCLSRQLV
                     NQFNTLKSNEEELNDIFINHYSLEKELTKEVQDEYISARLPDKAREIKDFISYSIGCI
                     FGRYSLDEDGLIFAGGEWDPSRYQKFKPDTDNVLLITDDEYFKDSQIDIVNRFIDFVE
                     ITFGKETLEENLQFIADTLGGKGTSREIIRRYFVQDFYKDHLKTYSNRPIYWQLDSGK
                     QNGFKALMYLHRYTPDQLGKVRTEYLHELQKAYDNRIELRQQQISTSDNPKERNAMTK
                     EIVKIQKQLKEVREYDEKLGHLALKRIELDLDDGVIVNYDKLQRDPKTGEKFQIFAKG
                     VKEPKK"
     gene            complement(13682..17353)
                     /gene="brxC"
                     /locus_tag="ACG842_001769"
     CDS             complement(13682..17353)
                     /gene="brxC"
                     /locus_tag="ACG842_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BREX system P-loop protein BrxC"
                     /protein_id="HFQ0248309.1"
                     /translation="MLIKDIFAKPIDRNIQGVIKVGQAKDENVQQELEEYVVTKELQK
                     HFKTIFDAYQRSINTPTDKMGVWIQGFFGSGKSHFLKIISYLLANREINDKKAIDYFK
                     EDGKIKDPLTLATMERCSQINTDVILFNIDSKSDAGGKKDKNGIVNVFLKVFNEMQGL
                     STIPHLADFERRLIKEEKFDAFKAAFEEINGAKWQEERHEFDWIQDDIKEAALKIDFM
                     SEAAIDNFLEKASGNYLISIDDFANLVKSYLDSKEADHHIAFLVDEVGQYIGGNRELT
                     LQLQTVVEDLGRICEGKAWVLVTSQEAIDHVTNFDNNSDNDFSKIKDRFATTVSLSSA
                     NADEVIKERILKKKEFANDSLALQFETVQTELKNKLLFVDSPELKLFQDAENYAEVYP
                     FIPYQFNLLGQILTEIRNHSYQGSNLSSGERSLLAMFKEAAERNKENDTTSLVSLDQF
                     YYSLERWIDTAISRVITQATNNSHIQRPTDIDNFNVNVLRILFMIKYVDQAIKPNVEN
                     ITSLMIRNINEDRLETKKRVEEALDLLIKENFVRKNLDSYIFQTDEEQEITREIKNQN
                     ITDSDLIVELSKRIFDGVLDRNSFAYEKLGMSGGNKFKNRYTFTYAQVLDQRPYKQAG
                     NAEMTVNFISPYSDLAGQEAELALKSSRNLLFVELPSDKEFKDDIAMYARMNLYLTTT
                     YSSVVKNFDAIRSEKSGELQELSASINDQLNELLKSAKFFYNGAEIQLTGSDFKAKFQ
                     SALQKMAEEVYHKLNQIDAPKEKEDILYLVKESKNEIIEDPSNEQAVQEIFNFINDQK
                     RNHLTITMKTIRDRFETKQPYGFTELDVEWCIAKLFMSGKIDLLYNNVQISRMDDPNS
                     IVMIIQKRRESEKIKIEPSAVISPKAKKLVDEIGRELFQSRNIVDENNNEHTMENFKK
                     YLKGQLDNLRSYFDLQNTYTYPSAYEYEQIKKYLEVIVQTSDVEDFFYTIKKYRDELF
                     DWAETYDKIKNFHKQNSTQKKIWENAQDKIRLIEKSRRQTSSQEIDQLIHEMKTILKK
                     ADPYSDMMKLNELIQEFIEQYNQLLELESQEILNKLTEEKQATFKLLEGKDFKDQFVP
                     IVDQEFQELKAKIEQANTIDDLVFLSLNVAEQYLIFNQRFDNEENRRRQEEQARKQIE
                     AANLIRETEKDEIDSAAKAEEIAIVKPTIQQKTVPMRTLNVHVKTIKSAADIDELTAE
                     INQKLKEQLQENTEITIQF"
     gene            complement(17378..17953)
                     /locus_tag="ACG842_001770"
     CDS             complement(17378..17953)
                     /locus_tag="ACG842_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1788 domain-containing protein"
                     /protein_id="HFQ0248310.1"
                     /translation="MSLSIQEHLEKLNEVIQKEDFLLNRGLGNEVGYYIFDYDPKDEL
                     IVRDYINDISGTSVGDNYEVKVYNLYVIMLEYLEKRGFTEKAIKMEERRGLQKLVESI
                     GRLLKMDLPEEENVFFEYVDAHTPEKSVVFLTGIGELYPLIRAHSILNKMHQVFDRVP
                     VVMMYPGEYDELSLTILNTNKDDNYYRAFRI"
     gene            complement(17953..18552)
                     /locus_tag="ACG842_001771"
     CDS             complement(17953..18552)
                     /locus_tag="ACG842_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1819 family protein"
                     /protein_id="HFQ0248311.1"
                     /translation="MNKKYSAGLVSKRFWFSEFKLYIQLRLEGYTDIEIKEKNEHENI
                     FFAVSSNRQKEIYQNVKRRVSQLDEAGLQLFNQLNIDNQKLVNLISILMIDDLFNEFM
                     TEVYAENLRKGNLQLTPVDYRSFFTEKRRSSEDISKWKDYTIKRLIAAYQTNLIETGL
                     VRGEKEYGRMTPILLDKRLIQWLVDKNRKDIVLALGGIL"
BASE COUNT         5747 a         3562 c         2612 g         6736 t
ORIGIN      
        1 gttttattga tttaaatagt tcatattttt tatttatacc gcttatttac tacttgctgt
       61 actagaatct gaagtgaatg tactcaaaac attttcaaag tctttgtctt tgatttttac
      121 attagcgtct ttcaatactt cgccaattac tttattctgg aaggtactgt cacttaattt
      181 tgtgtctgtt gcaatttctt tcaattcttt tttgtattta tccatgtcgt tgcctttgtt
      241 ttggtttttc accattttaa ccacataata ttccgtagta taagttgaag cattggtaga
      301 agtgatcaca tcagaaactt gaccatcttt caacttaaag gctgcttctt tgacttctgc
      361 cggaaccgtt gtagtagtgg aatcaaattt cacttttccg ccatcttctt ttgtcgtagt
      421 atcagttgac ttgtctttgg ctaatttaga gaaatcgtct cccttatcag cagctttctt
      481 cacatctttt gcatcatctt cacttgctac ttggataatt tgagcttcca cttctggatg
      541 gaaagattcc caagccgttt ttaaatcttc atcggtcaat ttgatatgtt tctttagacc
      601 ttcttgaaag gctagacttt gtttgatact gtctttgaat gtttgttctg tgtaacctgc
      661 agatttcaat tgtgaatcaa atgaatcacc caattgtttc tttgtttgat catattggga
      721 atcgatttct ttggttgata cgtcatctcc atatttatct tcaaatactt gataaacgat
      781 caactgttgc acagcggaat ctgctccttg catttttttc aagctatcca ttacttcttg
      841 ttcgctgatt gtaccgcctt tataactaac ggctgttttt tcaccggcat tcgtacagcc
      901 tgacaataag ccagtgccta gtacgatggc aagtgctaaa aatctttttt tcatttatca
      961 attctcctac ttttcgtttt ttgcgaattg ctaaaaacaa aggtgttcaa gatgaaaatc
     1021 atcaagaaca cctttactaa tctgctcgat acgtcctgaa tagatataag gctacacaat
     1081 ccagaaagtt cgctcagcag aataaatgtc aagtagcaca tcacacctta atatactact
     1141 tcttttgtta ccgtttttca agtctatctt gcgttccgta tttaacgtaa ctaattctta
     1201 gaacaaatgt tatcgatttt gtttttcttc ttccgctgtt gggatattca aatcagacac
     1261 ttttatgttc gattttccag acattttttt gttgttttta ttagaaaaag tttgtttttt
     1321 catttgtttt ctaaagcatg atcttcttgt ttcctatttt ggtcagatat ggatcatgcg
     1381 tgaccacgat aactaacgta ccttgggaag cctgttgcga taagatttca acgacttgct
     1441 ccttatttcg tttatctaat gatccagttg gttcatcggc taaaatgatt ttgctattct
     1501 tcaataagac tctcgcacag gcgattcttt gcttctcacc accagataat gtgtagatag
     1561 gcgtacttag cttcttgttt atatttactt ttttcaatgc ctcatacttt gctgttcgct
     1621 tatctgaaac atttaaattc gaaaacttca atccaatatc taaattaaaa tcgattgttt
     1681 catattcaat caacccaaaa tcttgaaaaa gaaaagagag ggcatttctt tttatcaagc
     1741 gcttctctct tccagttaaa tgttttgttt cttgattaaa taaataaaat tgccctgaac
     1801 tgaattgatc attcaatcct aatatattca ataaagtact tttccctgct ccgctctctc
     1861 caactacagt cacaaaatcc ccagcatccg cagtgaaacg atccaaatga agaatcgttt
     1921 tgtttcgaat gatttttttc gcacctttta tcactatcaa cggctccaat tgttaacctc
     1981 cattcatcag caataactga cttttctttt ctgtagacaa aataaaaaca actgaaagaa
     2041 taaaatcaat gaagctaaag aaaaaaatat taaaaagata tttaggctct ctttctataa
     2101 gaaaaaggaa gattacactt gtcgttcccc atttgatgag gttcatcatg aagaaatctt
     2161 tatacgtttc aaagagcgaa taaccacttg aacgaaagat agcgattttc cgacaattgt
     2221 agacaaaata tatgcataac atgacatagc tcaacaagaa gaaagaaaag aaagtgagta
     2281 aaaatatctg tatcaagtaa tgaaatgatc tgcttaacac tttgacagct atctcttcag
     2341 tctgagtata cggaaatgat aactgcaagt tatcacgtaa ctgactttct tcaatcagct
     2401 caaataacct ttctttcgac aaagagccta tcttgatctt taacggtgga tagatttctc
     2461 cgctgagaat atttctttcc caatacgtac tgtttttcaa tgtcagtatt tcaacaaccg
     2521 gataatgttc gatccagggg atagaaggaa taaatgtata aatgggttga ttgtttttta
     2581 taaatagaaa tttcggttct ttttcactga tttcttgata atattctgtt atttgcgcca
     2641 ggtcattttt taaatctgta tgtctatatc gttcagggat caatataaca gggtgcgttt
     2701 ctttttcggt tatttggatc aaccttccag agacatcaat caaaggaaat ttcttaagat
     2761 aattcagatt tatttttatc cctctgccaa actgttcatt ttcttcattc aagtaatctg
     2821 atacattgac taatatactt ccgtttttat tcaaagtttc atataattcc tgatcggctg
     2881 ctgatgcaaa acgattggaa taaataaaat ccacgtggtt tttcccgatg gttactggat
     2941 aaaatacatg atagtcctca ccagcgattt cttttggtgt ttcatgggaa acagctaact
     3001 gtgaagaaga aaaaacgata gttgctaatt cgacattggc aacggtcaaa agaagagaaa
     3061 acaacaatcc aaatgtaaaa agaatgtatt ggatatagtt gaacgcttct cttaacgaag
     3121 cgattttttc aactaataaa agagaaatca aagagatacc ctatattccg ctgaacaaga
     3181 aaacgtacat ctggaacaaa taaagaaatt gctccttata atacataaaa ataaaaccac
     3241 caatgattcc tgcaattatg aatgtaataa tgattttctt tttccccaat aaacaccaca
     3301 atacttgatg taatgaaaag ccattattca acaataaact gatttcttta ttcatcaata
     3361 agaaaaagga tgataacaaa aggaataaaa gaatcaatcc aatagttaaa tacactcgat
     3421 aattatatac gtctgtactt gaaataaccg aatacgaata gctattttca aagtcttcaa
     3481 aaatattagt ggcataatca gtgtgaaatg cttgattgaa tcgttccaca aatagtaaaa
     3541 tgaaccgttc ataggcttct ttttctctcg tcatcaagaa aaattcacct tcaaaatctt
     3601 ttattttgat cgattcttca atggacgtta cgcgaaagga ggtattagaa tagggtacct
     3661 tctcgtgaga agatttgaca tccccttgat aatacaatgt catttcaagc tttggctgta
     3721 tcttgaagtt ccaagatgta tctacttcat tggattcatt tactgttttt ttcatataag
     3781 aaagctcagt ttctgatgag acattttcaa ataccatgat tagctgctca tattcttctt
     3841 gcccaataaa atgttgtggc aatcgaatcg gagtataagt atctgtttga tggaaaaatt
     3901 catttgtccg atagagtacc tgtttattta taataaccaa ggcaaaacca ctcatgatac
     3961 cggtcactat gattgtgagt aaaactgtga actttttttt gattatcacc actctccctt
     4021 acttaaatga ttctttgata aaatcatacc gtttttcatc aaaaaaacgt atctatcaaa
     4081 tgtataggtc atgtagaata aatctatata ctgtagaatc gaaaggagaa gaagatgaac
     4141 atcggagaaa aaatacgaac ttaccgcaga aattgtaagc ttacgcaaaa agaattaggc
     4201 gaaaaaatcg gtgtttccga taaaacaatc tctagttggg aaaattctcg tacaatgcca
     4261 gaccttgaaa tgctgtcact tttacatcag actttaggtc tgccgattga ttttccttct
     4321 attgaatcag ctaccgaatc aatagaacca acattgccgg atgaacctct tgttatcaag
     4381 aaatcttatt catcaaaaca acccttctca ctcaaaaaga cactatcttt ctttctgctc
     4441 gtcttttgtt tggtctatct ttttcttatc aattatcaat ataaaaatga aacggttgat
     4501 cgattcaatc cttttttgaa accacaaatc ggctatacca cactccctgt aggatacgtg
     4561 gaaaattcca tcgaacattg ggtatgtaat gatatctttg ttcatggtac atggctgtct
     4621 tttcacgctg gagaggtaga aaaaggtaag aatcatgctg taatcaaaca caaaggaaaa
     4681 cacgtcatga acgttcgcct gctcacttgg gaagccattc cctcagatta ccaagaaatc
     4741 ctcatccaag actatgacga tgccttaaaa agaagccctc aggagccaga taggcattaa
     4801 gtaggagatg aacgtagaga tagaaaaaat caatgcacat gatcttatca aaaaaaaaca
     4861 ggggtacaaa ttaattgaaa aagctttgag atgaaagttc ctaaataaaa aagttcagaa
     4921 tgataggtaa aaaaatgact aactattcta gtattaggct atttgtatta ttcttctgtt
     4981 atttttatat caaaaaatcc agaaataact aaatataggt atttctggct tatagtataa
     5041 tatttacgcc acagccccca atgattcttt ttggaatcat gatcaaggac ggacatacac
     5101 aaaaatattt tgcccattcg atccgaatag attaacgact aatctagtaa gtaatataga
     5161 ttggtacgta gtcaaaccag caaatggaaa agttgtctat tccaccccaa gggctttaaa
     5221 gacagcatct tccggcgttt gataaaaaat caaattaaag gtacccatta attctggggg
     5281 tacagttcca atatcagcag cgcttatcat aggtacaagt acttttttcg ctccggcatc
     5341 ttttgctact tgtaaacagt tagctaaatt atctacctta atcatagaac caccaattgt
     5401 aatatcccct aaaataacta aacttggtaa tacaggttta tttaaggaaa ctgagcataa
     5461 agctattata gttgataacg ataatgtttt tgtcatacca atgccattca tatcttgaac
     5521 atgaacgaca aaatcttttg tcgtcaaact aatagactga ctaacacttc cataattagc
     5581 tcttaaatat cggtatgccg aatccatcgc ttctcttgct ccagcatctg aattgatccc
     5641 agtacgttca aactttccat ttcccgaagt catttgcgtt tccaatttat aggtacctaa
     5701 ttgcccagtt ttgccataag ataccgtgta caaatgccct ggcttcccta acccttctgg
     5761 aatcaaagtt cctccacctt gttcaggaac agaaacatac tcttctcgct cttcttctaa
     5821 atcaatgtat gaaaaattga catcataaaa ttccattcca ccaattttct ttaattgttc
     5881 ttttacacga cgacgcattt caagagcaaa tcttagtatt tcttcgacat cacttttttc
     5941 aaatctacca tcaggataca acagtttcat catcccagat acaatccgac gaactgcaat
     6001 cgtatcccgc tgatttaatt gagaaccaag tttgaaatat ttatcaaaac tatcgccaaa
     6061 actaactttt cgaagctcac gcatgacttc agaaaaataa tctgtaatga agccatagtc
     6121 atttgtaaaa aaatctggtc gatatttagg aatttcccaa cctggtaaat aacaatgcat
     6181 ccgatccaaa aaggctgaat ccgtccccat aacctccgga aatggctcaa ataaattcga
     6241 agttttcaat attacatcaa cgctttgatt aatatttcct acaaaagcca ttgatgctga
     6301 agcattcttt tcttctttgc ctctagcaaa agatcctgaa gccatgtaat ctttcatgat
     6361 ttgaacgcca tcattatcct taaatttaat cccagcaact tcgtcaaaag caacacagtc
     6421 ccacatacct acaagtccca ttgtacgact agccatatta tagaaaaggt ttgctactgt
     6481 tgtttgtcca cctgaaacaa ggattgagtt tggtgaaatt tctttatata catgcgattt
     6541 acctgtacca cgtggtccta gttcacagag attaaaatta ttttcaatta atggaataag
     6601 tcttgctaaa tgaagccatt ttaccctttt attaaattgt gtaggttcca taccaatgga
     6661 ccgtagaagt aagtcaatcc attcatttgt agtaaactcc ttgcgagcca tcttgtaagc
     6721 ctcaatgtct aaaccaggca tttggattgg atcaaccttt tttgcaataa aagggctatt
     6781 ccaacgatca ttctcatcat accaatattc catcgaaaca atacaccaga tccccccacc
     6841 taataatcga tcaaattttt tcggatactc ttcactaatt tgaacatttt ttaatcccag
     6901 gttagagaat tctgcttcat aacaatcttt cttttcattt aaatgtacag ttactttatc
     6961 aataactgta taaccaccca attcacgaat ttttgctttt actttttctg cttcatcagg
     7021 acgcacatag tttcgagcaa gaatattttt aacattttcc acaccttttt caaccaatgt
     7081 ttcatccaat gacgaagcat acataccaag taaatattcc aatacataaa caggtacatt
     7141 cgcaccttct ttaattttct ttgttaaatc ttttctgaca atctttccgg gaaagaattc
     7201 agataacttt aaaatttcct gttgttccac tagaattcct ccatgtgttc agtcattata
     7261 atagcaatta aagtatatca tacttaacca gtaggcttga taatacaaaa gaaaaagtat
     7321 ccacaaaaaa ttgtggatac ctcgttttta tatatcaaaa tcaaatgatt ctgcaaaagg
     7381 attatctata atgaatttta ttcgctcggt caaatcattt ttttgatttt ttatcactaa
     7441 ataataagta tcttcaactg tatatcgacg atttacaaat tcaaaaacaa atttagtaaa
     7501 ccgctgattt gcatcttctg tttttgaatc cgcaacatag atttcttcat ttgagatacg
     7561 ttcaccatat tcatcttcaa aataaagcca gaaagatgcg gggtcataaa catcactaat
     7621 tgcatttttt tgatagaatt ctaacgtggt tgataatcct gtaattctgc ggttgctagt
     7681 cattaattca attgctactg gttcctttgc attgctacct ttggctaccg taatttctag
     7741 aacagggact agcatttctt gaatagatga ccctccatga acgtagcttt gtcccccgcc
     7801 agctttctta aaaatacttg aagtaatcgg gacacaaatg tttctagtgt catcatttcc
     7861 taaagaatct cccaaagagt aatttttcgt accaattatt tcgtattctt cttcactaag
     7921 aataaaacga cgttctacac gatctttttc atttctagat ggattttcaa tcttatctac
     7981 ttcttcaacg tggcttcttt tataaataaa tccatgatca gccgtaacta taaaacgata
     8041 gatactgcca tcattatgca agcgattaat aagtcgggaa atttcgctta tcgctaaatc
     8101 acaggcctca aaaactcgat tttcattcga gtgatcacct gttctatcca cttgatcgtg
     8161 ataaatataa atgactgatt tgccattaaa tacttctcgt acctcagcct tattcatctt
     8221 aaccacatca tcataattta aagctacagc atttgcatct cgttgctgga gaatcgcatt
     8281 tcgattggat aaaccattcg tttttttccc atcaatcagt acttctgttc cttcattgta
     8341 agttagttga ttgtttggta ataaggcggc tttaccgaat tcagtaactg aaggtaatac
     8401 agaaagaaag ctagccatgt ttgttgaata cttcttttct ctaactaact tacgttctaa
     8461 attcatccct aactcatatc gaaaagcatc tgaaataata actacagttt taacagagcg
     8521 tttcattaca gcttgattat aaaattttcg aatggatgga cgattcttaa attcaaaagt
     8581 ttgattccaa atttgtcctg attgatcaag aaacttcgaa taatttgctt caactaatcg
     8641 atacaattgg ccatattttt gcttttcttc tatatgatca ctattccaaa taaatttccg
     8701 ataatgcgta tcaacccaat acatttcctt ctcatattga gttagcaaat tatccatgtt
     8761 catcggcaag cacaatctgt ttttatccac aagataataa gcatgtttca ataattgata
     8821 ctcataaata taatccgatg cgaaatgcat tttttcgcgc tgctttaaac tatcaaaaat
     8881 tgtttgtcct gcaattttcg ttgtagtgtc agaagccttt aatcgttctg cataaaacat
     8941 taatattttt gcatgaatct cagaaaatac gtctgcttca attagatcat caatttctat
     9001 actgtctaat aatccatcac catcaatagc ctgatatact tttttagata gctgttcaaa
     9061 gctttccatg taacgtgtat cattcataaa ctgatccatg aaagtaataa tgtttgcttt
     9121 caaatgtagt atttcaaatc ctttgaactt cttcggcatc tcttgatcaa tttgataata
     9181 aaaatagttc aaataaagac acatcgtcag cttttccaat gttggctggc tatccgtaaa
     9241 gccaaatttc atttgaataa attcccaaaa tttagctgtt aactgatact tatcaagttc
     9301 ccttaaagga ttctccctac tttctgtaaa gctttttaga actacacgca gtaaatcatt
     9361 taatcgaata cttttcgatt tcaacaaaac agccattatc attaattcaa tcgtttctga
     9421 tttattaatt gtcatatgcg tactaaattc accaaaactt ctcatgcgat cctttgcatt
     9481 aaagaaatta cgatactgtt taactgtctg atgaaatcta agtggaatag ccaattcatt
     9541 catgataatc gctaagcggt ctgcactaaa ttcctctgaa taattcaata cactcaacaa
     9601 atgattatct tcatctttat catcccaacg aggaaatggc gcatagatta aatagttagt
     9661 ttcgggatct tcatattcca ataaacgctt agttttaaat tgtgcgtcct tttctaatcg
     9721 atagattttt gcattttgaa tgaccaatga gtcaatttct tcaagaaaat cctgatttga
     9781 atcaaaccaa aaaatgattt tcctacatcc ataattagga aaacgttgat ttaatttttc
     9841 ttcaatttgt tgcgtattta tatctgtcat caatctcacc taatcgttct ctttctatac
     9901 cccttatatt gacataattt ggataatttc gcacgcaatt taagaataaa aaagaaagtt
     9961 gttagaaaac aactttctta gataactatt tttttggttc tttgacccct tttgcaaaaa
    10021 tttggaattt ctcgccggtt ttaggatcac gctgcaactt atcataattg acaattacac
    10081 catcatctaa gtctaattca attcgtttta atgctaagtg acccaacttc tcgtcatact
    10141 cacgaacttc ttttaattgc ttctgaatct ttacaatttc ttttgtcatt gcatttcttt
    10201 cttttgggtt atcacttgtt gaaatttgtt gctgacgcaa ttcgattcga ttgtcatacg
    10261 ctttttgtag ttcgtgtagg tattcagtac gtacttttcc taattgatct ggtgtatagc
    10321 gatgcaagta cattaacgct ttaaagccat tttgttttcc tgagtccaat tgccaataaa
    10381 ttggtcgatt ggagtatgtt tttaaatggt ctttgtaaaa atcttgtacg aagtaacgac
    10441 gaataatttc tcgacttgtc cctttgccac ctaaagtgtc agcgataaac tggagatttt
    10501 cttcaagggt ttctttacca aaagtaatct caacgaaatc aatgaaacga ttgacaatgt
    10561 caatttggct atccttgaaa tactcatcat cggtgatgag taaaacatta tcagtatcag
    10621 gtttaaattt ttgatagcgg gaaggatccc attcgccgcc agcgaagatg agaccatctt
    10681 cgtctaggga gtaacgaccg aagatacaac ctattgaata agaaataaaa tccttaattt
    10741 ccctcgcctt atcaggtaac cttgcagaga tatattcatc ttgaacctct tttgttaatt
    10801 ctttttcaag agaataatga ttaatgaata tgtcgttcag ttcctcttca ttcgatttta
    10861 gagtattaaa ttgattaacc aactgcctac ttagacagtt aaatgcctct gagagatctt
    10921 ttttaaacat ataacttaac aaaatgtttc ttctaaactc gtatgacgtt tcaagagcat
    10981 tatagtcttt tctagctata tcgatatttt catccacttt ttctcttatt ttttttaatt
    11041 ttctctcaga tgtaacttta agaaaaggta gttttcccat tggtccttca tggtaatcca
    11101 aggtgggaga aataaaagtt aaaaagtgat taactaattt agagtttaca tagccaaata
    11161 tttcttcaaa gtcagttata tcctttgaga acagaacaga tcctttagtt tcaaaaattg
    11221 cctgattctg caatctaaaa gatacttgtc cagatgaaag tgtcgaccaa gtaattccct
    11281 ttttaaaata gtaattaaga ttttgtggtc tagattttaa cttcccattt tcattaacat
    11341 aattttttat ttcatctcca tcattctccc agttaaccac gtattcattg tttccatacc
    11401 attttctaaa acgaccacct ttagttgtag gaaaccattt ttttctagac tttttagcgt
    11461 cctctaatgt tttagcatca aactttattt tgttaaattc tacttcaaac caataccgca
    11521 aaaatttatt gttatttccg gttgccaacc cttgttttgc atctgcaaca tcgttagtta
    11581 attttccttg agcaaaatca gtgattacgt tatcgcttgc ccagtaagca atcggcattc
    11641 ctgggatttt tttgaagttg tcttgattag tactataaaa ataatcgcat tcaccatgct
    11701 gatgaattgc ttctaacgtt ttaatttttt gaacttccat tccaccttta aaatctgata
    11761 attttatata gcttccatct tgttcatttg aattttttat cgtaaacgta caaattggaa
    11821 cggttgcttc ttcaaaagct gaatactcca tttgaatcaa actagaaatg tttctttcat
    11881 taattaaaaa tgagcgtaac ttttcatagc tagaaatgaa catccaaaca aatggcgtca
    11941 taaaggaagc atatccatta tcaatcagca tttggctatt caatttaata aacacagaaa
    12001 atagatcttt tttgacatca gaatagttat catttacata tttttttaat ttcttatcca
    12061 ttttattaag ataaggcgga ttagtgacta ccgctacata tttatcaact agcatttcag
    12121 ccacgtcaag aatgtgcaag tatttttctt gtatttcctt catcccataa agaatatcca
    12181 tattcgtaaa ttcgttgaat tttgatatat actctcttac ttttcctaag tattctttag
    12241 ttattgcctg attattttta tcgactaact taatgattga tccaagttct ttcccatttt
    12301 caaattgatc aaaaattaac tctttgtatt cattccagcg ttcactatac ttatcattgt
    12361 tcaattcact aatacgctct tgatactcgt ctgtcacttt aggagaatca ataaactctc
    12421 ttaaattcgg cgatagactt tttttcaaaa aaagacgatc gtattgacgc cctttcatca
    12481 taattgcaaa ataagacaat tgatacgctc ttttatcgat ttcaagacca tataaattgt
    12541 attcaacgat tgcacgtgca gcatcacgct cagaatatcc taacgtaaga taaatttcca
    12601 tcaatacatc gaaggcgtaa actaaaatat gcccagaccc cattgcgtta tcgataattt
    12661 ttagttcttc tggttttaaa ttacttacag atgttgcttg ttcaaaagca tatttatctg
    12721 gaattaagta tttcaactta tttcttaatt cactattggg ataatgatct aaccaatact
    12781 ttcctaaact attgtcaacc atataacgaa cgacccaatc tgtggtaaat agttgagttg
    12841 ctgccggaat atcttgtttt ttgacaatcc ctttataaat attcaccact tgattatgtg
    12901 gctcactatt atagtactga tacaaccacc caataatctc tacctgtcca ccagcttgaa
    12961 catagaaatc atcctctgga atacttaaaa gatcagcgat tacaccatct tttttaatat
    13021 aagaagctgt aaacaaaagt tctgtataat cttctacttc ttcaaatagc tcaggtaagt
    13081 aatcattcaa ttgattacag atttttataa aaatcaattg atacagcttg tccattgctt
    13141 ctgctgaatt atctgttttc caagttctga cttgttgctg ttcattacta gataattctt
    13201 caaagatatc ggcatcataa atatctgtaa taatatctgg ctcaattttc ccacttattt
    13261 cactagataa tacacgatac gtgtcaggta aataatcatt tacctccata aaccgaatgg
    13321 caatcaatcg gttaaaccac gtataagcaa cctcttcgat taactcttga aaagcttctg
    13381 aataagaagt gacagaagca cgttgtttta gttctgcaat caatttgttt ctttgcgcta
    13441 tttcttttcc ttgaattctt attcctgatt tttcgatttc ttgtaaattg ctatcaattt
    13501 tcttcacatc ttcaattttt gattcatcaa ttcctaaacg ttttgcttta gtcattactt
    13561 catcaattaa ttttctacgg gcattgaccg cgaaattctt tatcgctttc ttatccattt
    13621 ttctttccct tctatatgta acctttgaaa agacaggcac ctttttatgc ctgtcttttc
    13681 actagaattg aatcgtgatt tctgtatttt cttgaagttg ttccttcagt ttttgattaa
    13741 tttcagctgt taactcatca atatctgctg ctgacttaat agttttaaca tgaacgttaa
    13801 gtgttctcat tggtactgtt ttctgttgaa tagtaggttt tactatcgct atttcttcag
    13861 cttttgctgc agagtctatt tcatcttttt cagtttcacg aatcaagttt gctgcttcaa
    13921 tttgtttacg tgcttgttct tcttgacgtc tgcggttttc ttcattatcg aaacgttgat
    13981 taaagatcaa atactgttct gctacgttta atgataagaa aacaagatca tctatagtat
    14041 tcgcttgttc aatttttgct ttcagttctt gaaattcttg gtcaactatt ggtacaaatt
    14101 gatccttaaa atcttttcct tctaataatt taaatgttgc ttgtttttct tccgttaatt
    14161 tatttaatat ttcctgactt tccaactcta acagttgatt atattgttca ataaattctt
    14221 gaatcaactc atttagtttc atcatatctg aataaggatc agcctttttc aaaatggttt
    14281 tcatctcatg aatcaattga tcaatttctt ggctagaagt ttgacgccga cttttttcga
    14341 ttaatctaat tttatcttgt gcattctccc agattttttt ctgagtacta ttttgcttgt
    14401 ggaaattttt aattttatca tatgtttcag cccagtcgaa taattcatcg cgatattttt
    14461 tgattgtata aaaaaagtct tcaacatctg acgtttgaac aattacttca agatattttt
    14521 ttatttgctc atattcataa gcactagggt aggtataagt gttttgtaaa tcaaaataag
    14581 agcgtaaatt gtctaattga ccttttaaat atttcttgaa attttccatt gtatgttcat
    14641 tgttattttc atcaacaata ttacgacttt gaaacagttc tctaccgatt tcgtcaacca
    14701 atttttttgc ttttggcgaa atcaccgcgc ttggttcaat ttttatcttt tcactttctc
    14761 gtcgtttttg aataatcatt acaatactat ttggatcatc catccgtgaa atttgtacat
    14821 tgttataaag taaatcaatt ttaccagaca tgaaaagttt agcgatgcac cattccacat
    14881 caagttctgt gaatccatac ggttgttttg tttcaaaacg atcacgaatt gttttcatcg
    14941 taatcgttag gtgatttctc ttttgatcat tgataaaatt gaaaatttcc tgaactgctt
    15001 gctcatttga tggatcttca ataatttcat ttttgctttc tttcactaaa tataagatat
    15061 cttctttttc tttaggcgca tcaatttggt ttaatttatg gtatacttct tcagccattt
    15121 tctgaagtgc tgattggaat tttgctttaa agtcggagcc tgtcagttga atttctgcac
    15181 cgttatagaa aaattttgca gatttcaaca gttcatttaa ttgatcgttg atacttgcac
    15241 ttagttcctg taattcacca gatttttccg aacgaatcgc gtcaaagttc tttactacac
    15301 tcgaataagt cgttgttaaa taaagattca ttctagcata cattgctata tcatctttaa
    15361 attctttatc tgatggcaat tctacgaata ataagttacg ggaactctta agcgcaagtt
    15421 ctgcctcttg acctgctaaa tcagagtatg gtgaaataaa attaactgtc atttcagcat
    15481 ttccagcttg cttgtatgga cgttgatcta acacttgtgc ataagtaaac gtataccggt
    15541 ttttaaattt atttccacct gacatgccta atttttcata ggcaaaacta tttcggtcta
    15601 gaacgccgtc aaaaattcgt ttagatagtt ctacgatcaa atcactatct gtaatatttt
    15661 gatttttaat ctcacgagta atttcctgtt cttcatcagt ttggaaaata taactatcta
    15721 agttcttccg cacaaagttt tccttaatta aaaggtccaa tgcttcttct actcgttttt
    15781 tagtttccag gcggtcttcg ttgatatttc ttatcatcaa gctcgtaata ttttcaacat
    15841 ttggtttgat ggcttgatca acatatttaa tcatgaataa aattcgtaag acatttacgt
    15901 taaagttatc tatatcagtt ggtctttgaa tatgagaatt atttgtagct tgtgtaatca
    15961 ctcggctaat tgctgtatca atccaacgtt ctaacgagta ataaaactga tctaaagaaa
    16021 ccaaactggt tgtatcattt tccttatttc gttcagcggc ttctttaaac attgctagta
    16081 atgaacgttc accagacgat aagtttgatc cttgatagct gtgattacga atctcggtta
    16141 gaatttgccc caataaattg aattgatagg gaataaatgg ataaacttct gcgtaatttt
    16201 cagcatcttg gaagagcttt aattcaggac tatcaacaaa gagtaattta tttttcaatt
    16261 ctgtttgaac agtttcaaat tgtaaggcta aagaatcatt ggcaaactct tttttcttca
    16321 aaatacgttc tttaattact tcatcggcat ttgctgaaga taaactcaca gttgtggcaa
    16381 aacgatcctt gatcttagaa aaatcattat cagaattgtt atcaaagttt gttacgtgat
    16441 caatcgcttc ctgacttgtc actaataccc aagccttacc ttcacaaatt cgacccaaat
    16501 cttccacaac ggtttgtaac tgcaaagtca actcacgatt accgccgata tattgtccga
    16561 cctcatcgac tagaaaggca atatgatggt ctgcttcttt ggaatcaaga tagcttttaa
    16621 ctagatttgc aaagtcatca atgcttatta aataatttcc tgatgctttt tctaaaaaat
    16681 tatcaatagc ggcttcactc ataaaatcga ttttaagtgc cgcttctttg atatcatctt
    16741 ggatccaatc aaactcatgg cgctcttctt gccattttgc cccattaatc tcttcaaaag
    16801 cagctttgaa tgcatcaaac ttttcttctt taattaaccg acgttcaaaa tctgctagat
    16861 gtggaatcgt gcttaatcct tgcatttcgt taaatacctt taaaaaaaca ttcacgattc
    16921 cgtttttgtc ttttttacca cctgcatcac tttttgaatc aatattaaac agaataacat
    16981 ctgtatttat ttgtgagcac cgttccatcg ttgccaaagt cagtggatct ttgattttcc
    17041 catcttcttt gaagtaatca atggctttct tgtcatttat ttctcgattt gcgagcaagt
    17101 aagaaataat ttttaagaaa tgagatttac ctgaaccaaa gaacccctga atccaaacac
    17161 ccattttatc tgttggtgta ttgatactcc gttggtaggc atcaaaaatt gttttaaagt
    17221 gtttttgtag ttctttcgta accacgtatt cttctaattc ttgttgaaca ttttcatcct
    17281 ttgcttgacc gaccttaata actccttgaa tatttcggtc aattggtttt gcaaaaatat
    17341 ctttaattag catttaatct cctaccttcc atcccaacta aattctaaat gctcgataat
    17401 aattatcatc tttattggta ttaagaattg tcaaacttaa ttcatcatat tcccctggat
    17461 acatcatcac aactggtacg cgatcaaata cctgatgcat tttatttaaa atagagtgcg
    17521 ctctaattaa tggatataat tcgccaattc ctgtcaggaa cacaactgat ttttctggtg
    17581 tatgggcatc cacatattca aaaaatacat tttcttcctc aggtaaatcc attttcaata
    17641 aacgaccaat gctttccact aatttttgta aaccacgtcg ttcttccatc ttaatcgctt
    17701 tttcagtgaa accgcgtttt tctagatact ctaacataat cacatagaga ttataaactt
    17761 tcacttcata attatcacca acagatgttc cagatatatc attaatataa tctcttacaa
    17821 ttaactcgtc tttcggatcg tagtcaaaga tataatagcc tacttcatta cctaaaccac
    17881 gattcaataa aaaatcttct ttttgaatta cttcattcaa cttttccaaa tgctcttgaa
    17941 tcgataatga cattataaaa ttcctcccag tgctagtaca atatctttac gattcttgtc
    18001 aactaaccat tgaatcaatc gtttatctaa taatatcgga gtcattcgtc catattcttt
    18061 ttcccctctg actaatcctg tttcaattag attcgtttga taagcggcaa taagccgttt
    18121 aatcgtataa tctttccatt ttgaaatatc ttctgaactt ctccgcttct ctgtaaaaaa
    18181 agaacggtaa tcaactggtg ttaattgcaa atttccttta cgtagatttt cagcatatac
    18241 ctcagtcata aattcattaa aaagatcatc aatcataagg atggaaatca aattcactaa
    18301 cttttgatta tctatattta actgattgaa aagttgcaat ccagcttcat ccagttgact
    18361 aactctcctt ttcacgttct gatagatttc tttttgacgg ttactgctca ctgcaaaaaa
    18421 aatattttca tgttcatttt tctctttaat ttctatatct gtatatcctt ctagtcgtaa
    18481 ttgaatatat aacttgaact ctgaaaacca aaagcgttta gaaactagac cagcagaata
    18541 ttttttattc aatagattgc ctcctaatca tctataattc aataagtaaa caagtgtttt
    18601 ttgctcatcg ttagcattca ttttttagta tctactatac aatacatatt tattata
//
LOCUS       DAZKLM010000056        18534 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3764
            SAMEA115706913-rid22582563.denovo.057, whole genome shotgun
            sequence.
ACCESSION   DAZKLM010000056 DAZKLM010000000
VERSION     DAZKLM010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706913
            Sequence Read Archive: ERR13196647
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18534)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18534)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287823.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287823.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,004
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,849
            CDSs (with protein)               :: 2,849
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 41 of 96
            Pseudo Genes (incomplete)         :: 52 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 15 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18534
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706913-rid22582563.denovo.057"
                     /isolate="VRE3764"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            172..516
                     /locus_tag="ACG842_001772"
     CDS             172..516
                     /locus_tag="ACG842_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HFQ0248312.1"
                     /translation="MAEKQTAGRDQLGDFAPKFAELNDDVLFGQVWSREELSAHQRSI
                     ITISALISGGNFEQLPAHLKIGKQNGITSEEIAEIITHLAFYVGWPKAWSAFSLAKEI
                     YLEENSEDKKEA"
     gene            517..936
                     /locus_tag="ACG842_001773"
                     /pseudo
     CDS             517..936
                     /locus_tag="ACG842_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297629.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
     gene            complement(1028..1378)
                     /locus_tag="ACG842_001774"
     CDS             complement(1028..1378)
                     /locus_tag="ACG842_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0248313.1"
                     /translation="MEKELSKRIIDELLTYLYSHEITEIRIGVEFAEEGLFIEIQGTA
                     ETQPEDLDVLADLLNNPRDPSMEIYYQELLGNPRHDREDYHLLGSLIDDVEIMYAQPI
                     INIKIFRRASHSYY"
     gene            complement(1344..2675)
                     /locus_tag="ACG842_001775"
     CDS             complement(1344..2675)
                     /locus_tag="ACG842_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1576 domain-containing protein"
                     /protein_id="HFQ0248314.1"
                     /translation="MENETDVIYIHPQKRIVSQKRKYFYLGFTGVFFLFIGLLSNTPT
                     DNWSGLLTILTSPSNLLTDYFALGGFGSAFINVGILTLLSVLLAYRHKVILNGPLFAS
                     ILTVTGFSFFGKNLYNSISIILGVYLYAVFVNKPFSQYIMIGLFGSALSPVVSYITFG
                     MRFPLLIGILLGNLAGIAIGLLLPPLAAQTLVFHRGFTLYNIGFTSGLIAMTFTAVLR
                     LFSYSIVENTLVFNEYHFPLIWIIFGFFSLTVGIGFYYNSFRLLGIREIFDSSGKLTT
                     DFIANSGIGATLINIGLVGLMLSSYVLLVGGQLNGPVIGAILSAVGFSAFGCHLKNSF
                     PILVGIFIASLFGTFHEITSTGMLVAAVFGTGLAPISGFYGSFYGVIAGVLHIALVHN
                     VSTLHGGLNLYNSGFSTGFVAGILVPILDNFTAVRKEKKTLGKRIIKKNHR"
     gene            2931..3497
                     /locus_tag="ACG842_001776"
     CDS             2931..3497
                     /locus_tag="ACG842_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFQ0248315.1"
                     /translation="MRRKVYTKDQILKAAYDVVAKEGFKGFTARNIAKKMGISTQPIY
                     LEFKNMEDLKNTLLDSIFEQLKEDVFPIERTGDKIIDLGLNYIYFAKKHHSLYMALYI
                     DEHGDGKKMHDFSYNYFREIVKEDERYNQLSDEYVDALHTGTWVAVTGLASLMSSNIM
                     HPTEEQIIALIKRTIDSILALDNPEIIR"
     gene            3642..3729
                     /locus_tag="ACG842_001777"
     tRNA            3642..3729
                     /locus_tag="ACG842_001777"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3674..3676,aa:Ser,seq:cga)
     gene            4063..5037
                     /gene="bsh"
                     /locus_tag="ACG842_001778"
     CDS             4063..5037
                     /gene="bsh"
                     /locus_tag="ACG842_001778"
                     /EC_number="3.5.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase"
                     /protein_id="HFQ0248316.1"
                     /translation="MCTAITYATKDHYFGRNFDYEMSYNEVVTITPRNYRFDFRKVKN
                     LDKHYAMIGIAAGVSNYPLYYEATNEKGLSMAGLNFPGNADYKELQEGKDNVAPFEFI
                     PWILGQCSTIDEAKELLATINLVNIDFSEKLPLSPLHWLLADKEKSIVIESMKDGLHL
                     YDNPVGVLTNNPPFDYQLFNLNNYRSLSNGTPENHFSNQISLNVYSRGMGGLGLPGDL
                     SSVSRFVKATFTKMNAASGDSESESISQFFHILGSVEQQKGVCDTGERKYEYTIYSSC
                     CNVDKGIYYYRTYEDSQITAVNMNKEDLDRHELVSYPVIKEQQINYVN"
     gene            5362..7257
                     /gene="asnB"
                     /locus_tag="ACG842_001779"
     CDS             5362..7257
                     /gene="asnB"
                     /locus_tag="ACG842_001779"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HFQ0248317.1"
                     /translation="MCGIVGFVNDKDNKKTIINTMMDRIVHRGPNSSGEYIDKHVALG
                     FRRLSIIDLEGGTQPIYNEDRTKIIIFNGEIYNYQPLREELIAAGHVFQTHADTEVLL
                     HGYEEWGTELLQKIRGMFAFAIWDNEKNELFGARDHFGIKPYYYAEMNGTFMFGSEIK
                     SFLPHPDFNKELNKEALKPYMTFQYSPLNGETFFKDVYRLPEGHYYTYKDGKIDIQQY
                     WDADFETKETHSRQEWIEKIDETVQASIEAHTVSDVEVGSFLSSGVDSSYVTSVLKPD
                     HSFSIGFDDKTYNEAIEARKLTELLDLDNTAAVIDGDMSFKAFPLIQYHLDEPDSNPS
                     CVPLYFLANLASQSVRVVQSGEGADELFAGYQTYGFHTNSKFIRVIAQGLKKLPKGTR
                     YNLGRKIGKMKNFHGRIHLYESLAPAKEYFIGHARVFEESEASEVLTPKYQTAPSVDE
                     IMTVHYEKTEGIKDEVNKMQYVDLHQWMPKDILLKADKLSMASSLEVRVPLLDIEVMK
                     LAQQIPSKFLLNQNNTKDIFRQAANKHLPEKWSNRVKLGFPVPIKAWLKEEHGYEQVK
                     ALFEADFAKEFFDQEKIMKLLDDHHEGRKEEQRKIWTIFSFLTWYKVYFVDETIPQAE
                     AIDYVTV"
     gene            complement(7452..7898)
                     /locus_tag="ACG842_001780"
     CDS             complement(7452..7898)
                     /locus_tag="ACG842_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HFQ0248318.1"
                     /translation="MPWNMKDFPASMKNLDKLTRKKAIDIANALLDEGYPDNRAIPIA
                     IDQAKKWYEDASESEKRTFEKEKNPSKTDEHDTNPRAGKLLDSDVIVEYEEEQWIVKS
                     KGAKKASNHFDTKKEAIEKGKQVAQNKESTLFIYKKDGTKEKEIDY"
     gene            8126..8275
                     /gene="rpmG"
                     /locus_tag="ACG842_001781"
     CDS             8126..8275
                     /gene="rpmG"
                     /locus_tag="ACG842_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HFQ0248319.1"
                     /translation="MRVNITLECTSCKERNYLTSKNKRNNPDRLEKQKYCPRERKVTL
                     HRETK"
     gene            8363..8881
                     /locus_tag="ACG842_001782"
     CDS             8363..8881
                     /locus_tag="ACG842_001782"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0005542 - folic acid binding [Evidence
                     IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HFQ0248320.1"
                     /translation="MFISMWAQDKNGLIGKNGLLPWRLPNDMRFFREHTMDRILVMGR
                     KTYEGMGDLSLPYRHIIVLTTQPNFRTKENAEVMHSIDELLSYSQTISEDIYVSGGSR
                     IFQELLPYTGRIWRTLIDSTFEGDTYIGNIDFSDFALIEEYEGITDQENLYAHRFQKW
                     ERVKNRVASKEE"
     gene            complement(8958..9914)
                     /locus_tag="ACG842_001783"
     CDS             complement(8958..9914)
                     /locus_tag="ACG842_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFQ0248321.1"
                     /translation="MKLKTKVFTLSSILLLAGVLSACSPSSDKETATTANSTTSSNST
                     HTVTDTLGHKVEIPNAPKRIIGSYLEDYLIALGEKPIAQWTVGSGSIQDYLQDDLKDV
                     PTISYDLPYEKVLSFEPDLLLISSSATVEGGKYEQYSKIAPTYVVKNGTDVTWEEQLK
                     DVGKALAKEKEAEKIISDYQKSIKKTREELADKIENKTAAVLWVTNNSAFMVSKNRSS
                     GRIVYDDLEFGVPDLVEEVSKKATSDWSAVSSEKLAELDADYIILVNSDKDAAMFNEP
                     SWKNLKAVTDNHVLQFGPESSWLYNGPIASQEMVNDIKEKLN"
     gene            10080..10886
                     /locus_tag="ACG842_001784"
     CDS             10080..10886
                     /locus_tag="ACG842_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0248322.1"
                     /translation="MTRLRAADIAAGYENKKIIENMSVTIPEGKITSLIGPNGSGKST
                     LLKSFTRILPVKSGKIIVNNSDISTYSSKELAKKIALLAQTSEHPTGMTVEEVVSYGR
                     YPYQKLFSGMDTQDHEAIEWALDATGLAGFRMRDIATLSGGQTQRVWIAMALAQETDI
                     LILDEPTTFLDPAHQLEILQLLTEINQMKQTTILMSIHDINHASRFSDYLLAMKNGKL
                     ITEGTPEEVITKECMKKIFEIDATIVTLPRTNKPVFLTYDLLPKEQEDQL"
     gene            10883..11872
                     /locus_tag="ACG842_001785"
     CDS             10883..11872
                     /locus_tag="ACG842_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FecCD family ABC transporter permease"
                     /protein_id="HFQ0248323.1"
                     /translation="MRTMKKNRLLLLVCFVLLIIFFVALSNGAATVGKADFWQALFDF
                     DRSKQSHQIIRNIRLPRVAGAFLVGSCFALSGALMQGVTRNPLADSGLLGINAGASLA
                     MALSFAFWPKASDFSVFLMSLAGSFAVTLLVFGFLQFSKMGMNPLQLILAGVAISSFF
                     TAISQALTQFFNLQQDLAFWFVGGAANITWKQLAVLFPLYILAVLVSFSLGKAISLIH
                     LSETHALALGSHPKRTRLIVFCLVAVLAAIAVSLVGPVSFIGLLVPPVVRGMVGTDYR
                     YVLPVSFLVGGGLVMLADFTARMINPPFETPFGLVIALIGVPFLLIQVRREQR"
     gene            11869..12873
                     /locus_tag="ACG842_001786"
     CDS             11869..12873
                     /locus_tag="ACG842_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FecCD family ABC transporter permease"
                     /protein_id="HFQ0248324.1"
                     /translation="MKKIYWTFYFLLFVLVILSIGSLMIGTPFIYLDQLFATFIGKES
                     SSLALIVREFRVPRLLISLLAGACLGVSGYILQGVTRNELADSSILGINAGAGFLVML
                     YLGFFSHYSLPFLLLVVAFLGGVLAACCVYLAAYDRNGFLGMNRILLSGIAVNAGLSA
                     LTLLCTIQLSKENYGFVNAWLAGSIWGASWPYVWALLPWAVILIPLGGLYAQRIVLLS
                     FGQERAQGLGLNVAKSQKILLLLAVALASGSVAVTGSLSFVGLLAPHAAKLVVRKENN
                     WTFVLSGLFGSIFLLSADMVARVILLSGEVPTGILIALFGAPYFLYALFIRQKHALNS
                     "
     gene            13005..13547
                     /locus_tag="ACG842_001787"
     CDS             13005..13547
                     /locus_tag="ACG842_001787"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HFQ0248325.1"
                     /translation="MDQLKVRLRRKGIEKLKNLAKSGKKKEKEQRIYKQLFASEYWKS
                     AKVIGTTLSNEFELDTQPVIQRAEKEQKKIVVPKTLPKRQMAFYEMDEQSVLVRSSFG
                     VLEPQSEKLYLPEMIDLLIVPGIIYNRAGYRIGFGGGYYDRYLNNFHKKTCSLVFREQ
                     LNEQWQPESFDQKIQQLFID"
     gene            13567..14265
                     /locus_tag="ACG842_001788"
     CDS             13567..14265
                     /locus_tag="ACG842_001788"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HFQ0248326.1"
                     /translation="MNYQQQIKLRVWLRRPLWTYAFLGIQTAVFLMMELFPRLEIPYY
                     AGMYGPYLVHFNEWWRLVTPIFIHFGLMHFVMNSLILYFMGQQIEAIYGHWRFFLIYM
                     FSGIMGNTASFAFNEANVLSGGASTSIFGLFGALFILGFHFKHNPAIQQLVRHFLLFI
                     VLTFVFGLLDTSVDVWGHVGGLIGGLVLGNVLGLPKQQTSYSIHQRILSTLVFVFLFV
                     ICILLGLKKYGLLV"
     gene            14286..14498
                     /locus_tag="ACG842_001789"
     CDS             14286..14498
                     /locus_tag="ACG842_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HFQ0248327.1"
                     /translation="MESLYDVQQLFKKFGIFIYVGARIYDIELMTIELRNLYDSRLID
                     HDTYMTAWRILKREHRIEESRKKGNL"
     gene            14498..15460
                     /locus_tag="ACG842_001790"
     CDS             14498..15460
                     /locus_tag="ACG842_001790"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0051156 - glucose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HFQ0248328.1"
                     /translation="MDKKIIGIDLGGTTAKFAILTPEGEIQQKWSIDTNILDDGKHIV
                     PEIIESINHRLNLYNMKAEDFIGIGMGTPGSVDSEAGTVIGAYNLNWTEVQFVKKLIE
                     AGTGIKFAIDNDANVAALGERWKGAGENDPDVVFVTLGTGVGGGIVAGGNLIHGVSGA
                     GGEIGHITVDPEGFECTCGKRGCLETVSSATGVVRLGRFLAEEYAGDSKLKAMLDNGE
                     EVTSKDIFEMAQEDDPFALMVVDRVCFYLGLACGNLGNTLNPSSIVLGGGVSAAGEFL
                     RSRVEKYFKEFTFPQVRESTKIKLAELGNEAGVIGAASLALKFA"
     gene            15478..15873
                     /locus_tag="ACG842_001791"
     CDS             15478..15873
                     /locus_tag="ACG842_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFQ0248329.1"
                     /translation="MVLWVINIILLLIVLAIILWEVYLRVMAKRSATTLTEEEFREGM
                     RKAQVIDVREKDVFDAGHILGARNIPYTVLKDSLGSIRKDQPVYIYDQKKSLSIRTAN
                     KLRKAGYQEIYLLKGGYDGWSGKIKSKKV"
     gene            16036..16401
                     /locus_tag="ACG842_001792"
     CDS             16036..16401
                     /locus_tag="ACG842_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HFQ0248330.1"
                     /translation="MIKLKRVYLDAEKEDGYRILVDRFWPRGISKEKAQLDLWLKEIA
                     PSNEARKTFDHVPERFASFAKKYQQELIFEEEKKQAIFKLSQLMKEHPVITFVYGAKN
                     EHQNHAVLLKKFIEMEACK"
     gene            16398..18209
                     /locus_tag="ACG842_001793"
     CDS             16398..18209
                     /locus_tag="ACG842_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HFQ0248331.1"
                     /translation="MKIGIIGAGPRGLSVAERILHNNQDRHSLELFLFDPNGPGGRVW
                     RLNQPPELLMNSVSQQVTLFTDETLSSGGLVTQGPNLFQWSKGPATDYIEQTVTKNKQ
                     LIIEEINRLEKDQQSTRCLYGLYQRWFFSKLQEEFPASIQFVYSLVKKVSKRKDGYLL
                     QTDQQSYSIDQLVLASGHWENDETEEERVWVDYAKKNVLLYQLPSNPADVDLSKIPPK
                     ENVFLRGLGLSFFDYVGLFTVSRGGRFENEKGKLVYHPSGKEPIVYSGSRRGLPYYPR
                     GRNQKQGGAMAWPRLLTNENLEQWHQSGFLTGETFFDYLKKDVELFYYKKIIEEYHLP
                     IPKKNFEHDFLSLSSEECLGKHPALIPYRWDWSYLETPLAYQNEPFSEASRIFIEGQI
                     KEAEKGNCTGAFASTFDALKDWRDPIRQAIEWHVFSAKEYKEILWGWFTHLNSFLTIG
                     PPIIRTKELAALIDAGIFHLIEPPFDIQQKHNRYVISLQDKEISSRYLIEARLPQTSF
                     SRSSNPVIKNLKENKLVRPFSFSDNTGSYHSEAIDVERGTNRVIDASGEIQSDFYCYG
                     IPVEGVDWLTAAVARPYTDPWNLRQADKIAQLILTDK"
     gene            complement(18271..18438)
                     /locus_tag="ACG842_001794"
     CDS             complement(18271..18438)
                     /locus_tag="ACG842_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3042 family protein"
                     /protein_id="HFQ0248332.1"
                     /translation="MKKFVSGILVGSLATAAAVAGLVASVKKTVIDPIDEKEAMIEEN
                     RKKAMRKRVSR"
BASE COUNT         5960 a         3133 c         3823 g         5618 t
ORIGIN      
        1 ataaaaaaag cgttagcaac tatgcttaaa aagcatatcg actgaataaa ctaagcattg
       61 gacaggctga aagactacat ttaaaatgaa atgcgaagaa acaatagaaa ataaagttgc
      121 cttagaacca tagttgaatc acctaacagt cgtgaaggga gaataagaga aatggcagaa
      181 aaacaaaccg caggacgaga tcaattagga gattttgcac caaaatttgc agaattgaat
      241 gatgatgttt tatttggaca agtatggtcc agagaagaat tatctgctca tcagcgtagt
      301 atcatcacca tatctgcatt aatttcagga ggaaattttg aacaattgcc agctcatctg
      361 aagattggca agcaaaatgg cattactagt gaagaaatag cagaaatcat tacccattta
      421 gctttttatg taggatggcc aaaagcttgg tcagctttta gtttagcaaa agaaatctat
      481 ttagaagaaa atagcgaaga taagaaagag gcgtaaatga cgacaaaaca cgaagaaata
      541 cacaatggcg tgatttttcc agcaggtgaa aaaaacgaag cttacgctca agtatttacc
      601 gggtaaagct atctgaatat gttagtgaat gatcccgaag tttctgttgg tgtaggtaat
      661 gtaacttttg aaccaggttg tcgcaatcac tggcatgtcc ataaagacgg ctatcaaatc
      721 ttacttgtga ccggaggaga aggctggtat caggaagaag gaaagccagc aagacgatta
      781 atggctggag atgtcgtcgt aacgcacaaa gggattaaac attggcatgg tgcgacaaaa
      841 gacagttggt ttgaacattt ggcgatcact gctggtaatt cagagttttt agaacctgta
      901 acagatgaag tgtatgaagg attggaaaat caataatgtc aagcttgaac agctcctgaa
      961 agtagatttt aatcgaactt tcaggagctg cttttttgat tcagtctttt ataaatagaa
     1021 aatagggtta ataataggaa tgagaagcac gtcgaaagat cttaatattg ataatcggct
     1081 gggcatacat gatttccacg tcatcaatca aactacctaa cagatgataa tcttcccgat
     1141 catgtcgggg attacctaat agttcttgat aatagatttc catactaggg tcccgaggat
     1201 tgttcagcaa atcagcaagt acatctaaat cttctggctg agtttctgcc gtaccctgta
     1261 tctctatgaa aagcccttcc tcagcaaatt ctactccaat acgtatttca gttatttcat
     1321 gggaatataa ataagtaaga agctcatcga tgattctttt tgataattct ttttccaagt
     1381 gtcttttttt cctttctgac agcggtaaag ttatctagta tcggtacaag gatacctgca
     1441 acgaaaccag ttgaaaaacc actgttatat agatttagtc ctccgtgcaa agtgctgacg
     1501 ttatggacaa gagcaatatg tagtacacca gctattacac cgtaaaaact gccgtaaaaa
     1561 ccgcttatgg gcgcgagacc tgtcccaaac acagctgcaa caagcatgcc agtactggtg
     1621 atctcatgaa aagttccaaa caaagaagca ataaatattc cgactagtat cggaaaactg
     1681 ttttttaaat gacaaccaaa agcactgaaa cccaccgcag ataaaatagc accgatgaca
     1741 ggtccattta actgaccgcc aaccagcaaa acataagaag acagcatcag accaactaag
     1801 cctatgttga tcaaagtagc accaatacca gaattagcaa tgaaatcagt tgtcaatttt
     1861 ccagatgaat cgaaaatttc tcgaatcccc aaaagccgaa aggaattata atagaaacca
     1921 attccgactg tcagcgaaaa aaagccaaaa ataatccaaa tgagaggaaa gtgatattca
     1981 ttgaaaacca atgtattttc gacgattgaa tagctgaata acctcaaaac agcagtaaaa
     2041 gtcatcgcaa tcaacccaga ggtgaatcca atattataaa gtgtgaatcc acgatggaaa
     2101 actaaagttt gagcagctaa gggcggaaga agtagaccaa ttgctatgcc agctaaattc
     2161 cccaataaaa tacctatcag taaaggaaaa cgcataccaa aagtgatata actaacgacc
     2221 ggtgataatg cgctaccaaa cagtccgatc atgatgtact gagaaaaagg tttattgaca
     2281 aatacagcgt atagatacac acctaaaatg attgagatag aattataaag gtttttgcca
     2341 aaaaaagaga aaccggtaac tgttagaatc gaagcaaata agggaccgtt taaaatcacc
     2401 ttgtgccgat aagctaaaag cacagaaagt aaggtcaaaa tccctacatt tatgaatgcg
     2461 cttccgaaac cgcctaaagc aaaataatct gtcaaaagat tgcttggcga ggtaagaata
     2521 gttagcaatc cactccaatt atcagtagga gtattagaaa gcaagccaat aaaaagaaaa
     2581 aaaacacctg taaaaccaag ataaaaatac tttcttttct gtgaaacgat tcgtttttga
     2641 ggatgaatat aaataacgtc agtttcgttt tccattacac accttctttt tcattttctt
     2701 taactataac ttgaaatgaa agatttggac aactatcatt taggtcaaaa ctaaaaatgt
     2761 aagggaaccc attgaattta accaaatttt tttaattttg caaaaaaaag ttaaagaaga
     2821 ctgaaaaaaa atttaaaata taacgttttg agtaaaatgt ttgcaaaaaa tgctaaaaat
     2881 agggtacaat aaaacaacat atactgcagt aaagaagtgg gggagtttta atgagaagaa
     2941 aagtctatac caaggatcaa attctaaaag ctgcttatga tgttgttgca aaggaagggt
     3001 ttaaaggatt tactgcgaga aatattgcta aaaagatggg gatttcgaca cagccgattt
     3061 atctggaatt caaaaacatg gaagatttaa aaaatacgtt gctggattct atctttgaac
     3121 aactaaaaga agatgttttc cctattgaac gtactggtga taaaatcatt gatctcggtt
     3181 tgaactatat ctactttgca aaaaagcatc atagtcttta catggcattg tacattgatg
     3241 aacatgggga cggaaagaaa atgcatgatt tttcatacaa ttatttccgt gaaatagtta
     3301 aagaagatga aagatacaat caattatcgg atgaatatgt cgatgctctg catactggta
     3361 catgggtagc agtgactggc ttagcgtcat tgatgtcatc aaacatcatg catccaacag
     3421 aagaacaaat cattgcactg atcaaacgga caattgactc gattttagca ttagataatc
     3481 ctgaaataat cagatagtgt tctatcagta tgaatttgat cggtaagtcc gttggctgca
     3541 aactgtcaat ggactttttt gctaaaaaaa gcgatttctg ttatttatct attagaaata
     3601 ggcttgacat ttcgtgaaaa aagtttaaaa atagtactta cggaaggttg gcagagtggt
     3661 aatgcaccgg actcgaaatc cggcgaaccg gctaataccg gcgcgcaggt tcaaatcctg
     3721 taccttcctt atatcaaaga ttagaggttg tgaagaagct gtcctgcatc aagtaataag
     3781 aacggccaaa caaaaatagc ttttcctatt ttttcgtttg attgggctta ttaccgcaga
     3841 tgcaagcttt tgaacaccgt ttgttcggaa taaggggctg tgacaagact tttgtcacag
     3901 ccccttattg ttgtactttt aacctacaat attttttgat tccccctgag tactattttt
     3961 tataaatgct agtaaatcaa tcttctttgg cctttttttg ataaaaaaaa tactagatgt
     4021 aagataaaag tgtaaaaacg atttgtttta ggaggggaaa cgatgtgtac cgcaatcact
     4081 tatgcaacga aagaccatta ttttggaagg aattttgatt atgagatgtc ttataatgaa
     4141 gtggtcacta ttactccaag aaattatcgt ttcgatttta gaaaggtaaa aaatttagat
     4201 aaacattatg caatgatagg aatagcagct ggggtatcca actatccact ctattatgag
     4261 gctacaaatg aaaaagggtt gagtatggca ggactgaatt ttcctggcaa tgctgattat
     4321 aaagaattac aagaaggaaa agataacgtt gcaccatttg agtttattcc atggatacta
     4381 gggcaatgct caaccataga cgaagctaaa gaattattgg ctactatcaa tctagtcaat
     4441 atcgatttta gtgaaaaact acccttgtca cctttacact ggttattagc tgataaagaa
     4501 aaatcgatag taattgaaag tatgaaagat ggacttcatt tatatgataa tcctgtcggt
     4561 gtgttgacaa ataatccgcc atttgattat caattattta atctgaacaa ttatcgctcg
     4621 ctatcaaatg gaacaccaga aaatcatttt tcaaaccaga tcagcttgaa tgtttatagt
     4681 cgtgggatgg gaggattggg cttacctgga gatctctctt ctgtttctcg atttgtaaaa
     4741 gccacattta ctaaaatgaa tgctgcatca ggtgattcag aatctgaaag tatcagtcaa
     4801 ttttttcata tactaggctc agtcgagcaa caaaaaggag tatgcgatac tggagaaaga
     4861 aaatacgaat atacgattta ttcttcttgc tgtaacgttg ataaagggat ttattattat
     4921 cgtacgtatg aagacagtca aattactgct gtaaatatga ataaagaaga cttggatcgt
     4981 catgaattgg tcagttatcc agtcataaaa gagcaacaga taaactatgt caattaatta
     5041 atgaatagat ccgtttggta tcatattttc tgatacagtc ataattagac acgatcttta
     5101 ggtcgtgtct ttttttgctg gtttttttgg aaatagcatc cactaatatt tttttaacac
     5161 gaagagaata aggtagcctc aaaaaagaaa acatggtaaa ttagatattg ggactttatt
     5221 ttctgtaaaa acagaaactt tcatcttttg ttttcacgtg acgaaattgt aagagagaaa
     5281 atattcgtcc gctatttatt ctatgataag atgcatatga atgaaataaa cagtaatttt
     5341 atattaggat gtgaaagtta aatgtgcggt attgtaggtt ttgttaacga caaggacaac
     5401 aaaaaaacaa ttattaatac aatgatggat cgaatcgttc accgtggacc taatagttca
     5461 ggagaatata tcgataaaca tgttgcttta ggattcagaa gattaagtat tattgactta
     5521 gagggcggta ctcagcctat ttacaatgaa gatcggacaa agatcattat ttttaatggc
     5581 gaaatctata attaccagcc tttgagagaa gaattgattg cagctggcca cgtttttcaa
     5641 acacatgcag atacagaagt ccttttgcat ggttacgaag aatggggaac agaattattg
     5701 caaaagatcc gcggaatgtt tgcttttgct atttgggata atgaaaagaa tgagttattc
     5761 ggtgcaagag accactttgg gatcaaacca tattattatg cagaaatgaa cggaacattt
     5821 atgtttggtt cagaaatcaa aagttttctg cctcatcctg attttaacaa agaattgaac
     5881 aaagaagcgt tgaaacctta tatgactttc caatattctc cattaaacgg cgaaacattc
     5941 ttcaaagatg tctatcgttt gcctgaaggc cattattata cgtataaaga tggaaaaata
     6001 gacattcagc aatattggga tgcagatttt gaaacaaaag aaacgcattc ccgacaagaa
     6061 tggatcgaaa aaatcgacga aaccgttcaa gcttctattg aagcacatac ggtcagtgat
     6121 gtagaagttg gttctttctt atccagcggg gttgattcca gttatgtaac ctctgtattg
     6181 aaaccagatc attctttctc aatcgggttt gatgataaaa cctataatga agcgattgaa
     6241 gccagaaaac taaccgaact attggactta gataatacag cagcagtgat cgacggagac
     6301 atgtcgttca aagcattccc tttgatccag tatcacttag atgaacctga ttcaaatcct
     6361 tcttgcgtac ctttgtactt tttagcaaat cttgcttcac aaagtgtgcg ggtagtccaa
     6421 tctggagaag gggcggatga actgtttgcg ggataccaaa cgtatggatt ccatacgaat
     6481 tcaaaattca tccgtgtgat tgctcaagga ttgaaaaaat taccaaaagg aacacgctat
     6541 aatttaggac gtaaaatcgg aaaaatgaaa aatttccatg gacggatcca tttatatgaa
     6601 tcacttgctc ctgcaaaaga atattttatc ggtcatgctc gagtgttcga agaatcagaa
     6661 gcatcagaag tgttaacacc aaaatatcaa acagcaccat ctgtggatga aatcatgact
     6721 gttcattacg agaaaacaga aggaatcaaa gatgaagtca ataagatgca atatgtcgat
     6781 cttcatcaat ggatgccaaa agatatcttg ttaaaggcag ataaactttc tatggcaagt
     6841 tctttggaag tccgagtacc tttattggat atcgaagtca tgaaattggc tcaacaaata
     6901 ccaagtaagt ttttattgaa tcaaaacaac acaaaagata ttttcagaca agctgcgaac
     6961 aaacatcttc cggaaaaatg gtcgaatcgt gtgaaattag gattccctgt gccaatcaaa
     7021 gcttggttga aagaagaaca cggctacgaa caagtcaaag cattgttcga agcagatttt
     7081 gcaaaagaat tttttgatca agaaaaaatc atgaagttgc tagatgacca tcacgaagga
     7141 cgtaaagaag agcaaagaaa aatctggaca atcttcagtt tcttgacatg gtataaagtc
     7201 tactttgtag atgaaacgat tccgcaagca gaagcaatcg actatgtgac tgtttgatga
     7261 aaatcaaata tcaataaaaa agaggttttc tcctaccgtt taacactgag tgataagaaa
     7321 caatttcaaa aaacagctct tcccgtttta tagatattat gacttatggt tcggagttgg
     7381 cagaggaaca ccattgtttc gaacataagg gggttgtgac acgattttcg tcacaacccc
     7441 tttacatatg ttcaataatc gatttctttt tcttttgtcc catctttttt ataaatgaac
     7501 aaagttgatt ctttgttttg tgctacttgc tttccttttt cgattgcttc tttttttgta
     7561 tcaaaatggt tgcttgcctt ttttgcccct ttagatttca cgatccattg ttcttcctca
     7621 tattctacga tcacatctga atcaagcagt tttccagcac gaggattcgt atcgtgttca
     7681 tccgtcttgc ttggattttt ttctttttcg aaagttcgtt tttctgattc actagcatct
     7741 tcataccatt tttttgcttg atcaatggca attgggatcg cacggttatc agggtaccct
     7801 tcatctaata atgcatttgc gatgtcgatt gctttttttc ttgtcaattt atctaagttt
     7861 ttcattgatg caggaaagtc cttcatgttc catggcatat tagtatcccc ctttattaaa
     7921 aaacataatc tatataaaca agtgtaaggg tatttataca aaaagaaaaa gattttgttt
     7981 gagaagtaaa accgttgtat ttatcgtcaa gtagttttac ttaacaaaat cgtgaaaaac
     8041 actagcaatc tagaaaaaat catggtaaga taatcaagtc tgagagtcat cttagaggat
     8101 tcttgtaaaa atgggaggga aaaacatgcg cgtaaacatc actttagagt gtacttcttg
     8161 taaagaacgt aactatctaa caagcaaaaa caaacgtaac aatcctgatc gtttggaaaa
     8221 acaaaaatat tgcccacgcg aacgcaaagt tactttgcac cgtgaaacaa aataatgttt
     8281 ttaaagttct caattccttg ctggtcaaag aattgagact ttttttatct caaaacatct
     8341 aaaatcataa ggagcggata atatgtttat ttccatgtgg gcacaagata aaaatggtct
     8401 gatcggcaaa aatggtttgt taccatggcg attgccaaat gatatgcgtt tctttcgcga
     8461 acatacaatg gatcgaattt tagtgatggg aagaaagacc tatgaaggaa tgggggatct
     8521 ttcacttcct tatcgccata tcatcgtgtt gacaacgcag ccaaattttc gcacgaaaga
     8581 aaatgcggaa gtaatgcatt cgatcgatga acttttgtcg tatagtcaaa cgatctcaga
     8641 agatatctat gtttcgggtg gtagtcggat ttttcaagaa cttcttcctt acactggtcg
     8701 gatctggcga acattgatcg atagcacgtt tgaaggcgat acgtatattg ggaatattga
     8761 tttttcagac ttcgccctta tagaggagta tgaggggata acggatcaag aaaatctata
     8821 tgcccatcgt tttcaaaaat gggaaagagt aaaaaatagg gttgcttcaa aagaagaata
     8881 aaatctatgc gtctagtttg ttaataagaa aaaaagcata tcacgactaa tcgtgaagat
     8941 gcttttttta gttaaaatca atttagtttt tctttgatat cattgaccat ttcctgactg
     9001 gcaataggtc cgttatatag ccaactagat tcaggtccga attgcagtac atgattgtct
     9061 gtcactgctt taagattctt ccagcttggt tcattgaaca tggcagcatc tttgtcactg
     9121 ttcacaagga tgatgtagtc agcatctaat tcagctagct tttcagagga aacagcagac
     9181 cagtcagaag tggctttttt ggagacctct tccactagat caggaacacc aaattccaga
     9241 tcgtcgtata cgattcttcc acttgaccga tttttgctga ccataaaggc tgagttgttg
     9301 gtcacccaca acactgcagc tgtcttgttc tcgattttat ctgctagttc ttctcgagtt
     9361 ttcttgatgg atttttgata atcggaaatg atcttttctg cctctttttc ttttgccaaa
     9421 gctttgccga catcttttag ttgttcttcc catgtgacat ctgttccgtt ttttacaaca
     9481 taagttggcg cgattttgct atattgttcg tatttcccgc cttcgacagt agcggaagaa
     9541 gaaatcaaca gcaaatcagg ctcaaagctt aacactttct cataaggaag gtcataagaa
     9601 atcgttggga catcctttaa atcatcttgc agataatctt gaatggaacc actgccaaca
     9661 gtccactggg cgataggttt ttcccctagt gcaatcagat agtcttctaa gtaactgccg
     9721 atgatccgtt tgggcgcgtt tggaatctct actttatgtc caagtgtatc tgtgactgta
     9781 tgagtactat tggatgaagt cgtcgaattg gcagttgtag ctgtttcttt atctgaagag
     9841 ggggagcaag cgctcagtac tccagccagc agcaatatgc tagataatgt gaatactttt
     9901 gtttttaatt tcatttcttt cctcctaaca taattgagaa tctttctcat tggttattat
     9961 tcctgatatt tcgaacgctg tcaaccgctt catttggcta tagatatgaa agtagcgctt
    10021 gacgaaatgt tgaaacagcg tcataattga gaatgatttt cagcgaaagg atgggtgaaa
    10081 tgacgagatt aagagcagct gacattgcag ccggctacga aaacaaaaaa atcattgaaa
    10141 acatgtctgt tacgattcct gaagggaaaa tcactagttt gattgggcca aatggaagtg
    10201 gaaaatctac tttattgaaa agttttaccc gtatactacc tgtgaaatca ggaaagatca
    10261 ttgtcaataa ctctgatatc tctacataca gttcaaaaga acttgcaaaa aaaattgcat
    10321 tattagcaca aactagtgaa caccctacag gtatgacagt agaagaagta gtatcttacg
    10381 gacgataccc ttatcaaaaa ctgttcagtg gcatggacac gcaagatcat gaagcaatcg
    10441 agtgggcgtt ggatgctaca ggtctagctg gatttcgtat gcgggatatc gcaacgctgt
    10501 ctggtggaca aacccagcgt gtctggatcg ctatggcgct tgctcaggaa acagatatct
    10561 tgatcttgga tgaaccgaca acatttttag atccggcaca ccaattagag atcctacagt
    10621 tattgactga aatcaatcaa atgaaacaaa caacgatctt gatgtcgatt catgacatta
    10681 atcatgcttc acgtttttca gattatttat tagcaatgaa aaacggtaag ttgatcacag
    10741 aaggtacacc tgaagaagtg atcacaaaag agtgtatgaa aaaaatattt gagattgatg
    10801 caacgattgt cacgcttcca agaacaaata aaccagtttt tctaacctat gatttgttgc
    10861 caaaagagca ggaggatcaa ttatgaggac tatgaagaaa aatagactcc tgcttttggt
    10921 ctgttttgtt cttttgatta ttttttttgt tgctctttca aatggcgcag cgactgtggg
    10981 aaaagcagat ttttggcagg cgttgtttga ttttgataga tcaaaacaaa gtcaccaaat
    11041 catacgaaat attcgtttgc ctcgtgtggc gggagcattt cttgtaggga gttgttttgc
    11101 attgagcggt gcattgatgc aaggagtgac aagaaatcct ttagcagatt caggcttact
    11161 aggaatcaac gcgggtgctt cgttggcaat ggcccttagt tttgcttttt ggcctaaagc
    11221 ttctgatttt tctgtgtttc taatgtcatt ggcaggttct tttgctgtaa ctttgctcgt
    11281 atttggtttt ttgcagtttt cgaaaatggg catgaatcct ttacagctga ttttagctgg
    11341 cgtagctatt agttcttttt ttactgctat cagccaagca cttacccaat tctttaatct
    11401 gcagcaggat cttgcttttt ggtttgtagg cggagctgcc aatatcacat ggaaacaatt
    11461 agcagtattg tttcctttat atatacttgc tgttttggtt tctttttcat taggaaaagc
    11521 aatatctttg attcatttga gtgaaacaca tgcattagct cttggaagtc atcctaaacg
    11581 aacgcggttg attgttttct gtctagttgc agtgttagca gccattgccg tttcgttagt
    11641 cggacctgtt tcatttattg ggctgttggt tccgccagtt gttcgcggaa tggttggaac
    11701 ggactatcgt tatgttctac ctgtctcttt tttagttggc ggtggattgg ttatgttggc
    11761 ggattttaca gcgaggatga tcaatccccc ttttgaaaca ccttttggat tagtcattgc
    11821 attgattggc gttccgtttt tactgataca agtaaggagg gaacaacggt gaaaaaaata
    11881 tattggacgt tctatttctt gctttttgta cttgtcatcc tgtctatagg tagtttaatg
    11941 attggtacgc cttttattta cctggatcag ctttttgcca cgttcatagg gaaagaatca
    12001 tcttctttgg cgctgattgt tagagaattt cgagttcctc gattactgat cagtttatta
    12061 gcaggagctt gtcttggcgt ttctggttat atcttgcaag gagtaacaag aaatgaattg
    12121 gcagattcta gtatattggg cattaatgcc ggagcaggat ttttagtgat gctttattta
    12181 ggtttctttt cgcactattc gctgccgttt ctattgctag ttgtagcctt tctaggtggt
    12241 gttttagctg cttgctgtgt ctatcttgcg gcctatgatc gaaatggctt tttgggtatg
    12301 aatcgaatct tattgtccgg tattgccgtt aatgccggcc tttctgcatt aacattgcta
    12361 tgcacgatcc aattatctaa ggaaaattat ggatttgtga atgcatggtt agcgggatca
    12421 atctggggtg cgagctggcc ttatgtttgg gcattgcttc catgggcggt tatcttaatt
    12481 ccgctaggag gactttatgc acagaggatt gtattgctat catttggtca agaacgcgcg
    12541 caaggattag gccttaacgt ggctaaaagt caaaaaatcc ttttgttgtt agctgttgct
    12601 ttggcaagcg gtagtgtagc ggtgacaggt agtttatctt ttgtaggact actagctcct
    12661 catgctgcga agctagtcgt acgtaaagaa aataactgga cctttgttct gagcggtttg
    12721 ttcggttcca tttttctgct tagtgcagat atggttgcta gagtgattct actaagtggg
    12781 gaagtaccta caggaatttt gattgccctt tttggtgcgc catattttct gtatgcactt
    12841 tttatcagac aaaaacacgc gctgaacagt tgaaattcat taaaaaagga aggccaatca
    12901 tttctaggtc ttctttttat tctatttatg catttatttg aaagttcgat tgctattttt
    12961 ctttttttcc tataccattt tatgagagaa ggaagggtga gtttttggac caattgaaag
    13021 taagattacg ccggaaagga atcgaaaagc tgaaaaatct ggccaaaagt ggcaagaaaa
    13081 aggaaaaaga acagcgaatc tataaacagc tattcgccag cgagtactgg aagtcagcaa
    13141 aagtgatagg gacgacttta tcgaatgaat ttgaactgga tacacagccg gttatccaac
    13201 gagcagaaaa agaacaaaag aaaatagttg tacctaaaac tttgccgaaa cgtcagatgg
    13261 cgttttatga aatggatgaa cagtccgttc ttgtacgttc gagttttggt gttcttgagc
    13321 cacaatcaga gaagctctat ttgccggaga tgatcgatct cctcattgta ccaggtatta
    13381 tctacaatag agccggttat cgaatcggtt ttggcggtgg ttattacgac cggtatttaa
    13441 ataattttca taaaaaaaca tgtagccttg tttttcgtga acaattgaat gaacaatggc
    13501 aaccggagag ctttgatcag aagatccaac aattgtttat tgactaaaat gaaatgggag
    13561 gttttcatga attatcaaca gcaaataaaa ttgcgtgtat ggttaagacg accgttatgg
    13621 acctatgctt ttttagggat acagacagcg gtcttcctca tgatggaatt atttccgcga
    13681 cttgagatcc cttattatgc cggaatgtat ggtccttatc ttgttcactt caatgaatgg
    13741 tggcgtttag tgacaccaat cttcatccat tttggactga tgcatttcgt aatgaactca
    13801 ctgatactgt attttatggg gcagcaaatc gaggcaatct atggccattg gcgttttttc
    13861 ttgatttata tgttcagcgg gataatggga aatacagcaa gctttgcttt taacgaagcg
    13921 aatgttctgt ctggtggtgc aagtacttcg atattcggtt tgttcggtgc gttgtttatc
    13981 ttagggtttc attttaaaca taacccagcg atccagcagc tagtcagaca ttttctttta
    14041 tttattgtgt tgacatttgt ttttggtctt ctggatacct ctgtagatgt ttggggacat
    14101 gtcggtgggc taattggcgg attagtgtta gggaatgttt tgggtctacc gaaacaacag
    14161 acaagctatt cgatacacca aaggattctt tctacactag tttttgtatt tctttttgta
    14221 atatgtattt tactgggttt aaaaaaatat ggtttacttg tataatatga aggaagtgct
    14281 taaagatgga gagcttatat gatgtacagc agctgttcaa aaaatttggc atctttatct
    14341 atgtgggtgc acgcatctat gacattgaat taatgactat cgaattgcgt aatctgtatg
    14401 atagccgttt gattgatcat gatacataca tgacagcgtg gcgcatatta aaaagagagc
    14461 accgaattga agaaagccga aagaaaggaa atttgtaatg gacaagaaga ttattgggat
    14521 tgatttaggt ggaacaacag caaaatttgc aattttaaca ccagaaggag agattcagca
    14581 aaaatggagc atcgatacca atattttgga tgatggtaag catatcgttc ctgaaatcat
    14641 tgaatcaatc aatcatcgtt tgaatttata taatatgaaa gccgaagact ttatcggaat
    14701 cggcatgggt accccaggaa gcgtggacag cgaagctgga acggttattg gtgcgtataa
    14761 cttaaactgg acagaagttc aatttgtgaa aaaactgatc gaggcaggaa caggaatcaa
    14821 attcgctatc gataatgacg cgaatgttgc tgctttaggt gaacgatgga aaggtgctgg
    14881 agaaaacgat ccagacgttg tattcgttac tctaggaaca ggtgttggcg gaggtatcgt
    14941 tgctggcgga aatttgattc acggcgtgag cggagctgga ggagaaatcg gacatatcac
    15001 tgtggatcct gaagggttcg aatgtacttg cggtaaacgc ggatgtttag aaactgtatc
    15061 tagcgctaca ggtgtggtac gcctaggtcg tttcttggca gaagaatacg caggggattc
    15121 aaaactgaaa gcaatgctag acaacggaga agaagtaaca agtaaagata tctttgaaat
    15181 ggctcaagaa gacgatccgt ttgcattgat ggttgttgat cgcgtatgct tctacttagg
    15241 tttagcttgc ggaaacttgg ggaatacatt aaatccgtca agtatcgtgt taggtggagg
    15301 agtatcggca gcaggagaat ttttacgtag ccgtgtagag aaatatttta aagaatttac
    15361 tttcccgcaa gtaagagaat caacaaaaat caaattagca gagcttggta atgaagctgg
    15421 tgtcattggt gctgcctcat tagccttgaa atttgcttaa aaaaacggag gcattacatg
    15481 gttttatggg taattaatat cattttgcta ttgattgtgc ttgcaatcat cctttgggaa
    15541 gtctatctac gagtaatggc caaacgttca gcaacgactt tgacagaaga agaatttcga
    15601 gaaggaatga gaaaagcaca agtaatcgat gttcgcgaaa aagatgtgtt tgatgctggg
    15661 catattttag gtgcaagaaa catcccttac actgttttga aagattcatt aggatctatt
    15721 cgaaaagacc agcctgtcta tatttacgat caaaagaaaa gtttgagcat ccgtacagca
    15781 aataaattac gtaaagcagg ttatcaagag atttatttac ttaagggtgg atatgacgga
    15841 tggtctggaa aaatcaaaag taaaaaagta taaaacagat gattgtatga cagctttaaa
    15901 aaccgaatca atcattagat tttgaccaag atcacatgaa cggcgcaatg gatagttgtg
    15961 ccgttcattt tttttatttt gtgctattat ctacatatat aagctaagcg aaaaggtaga
    16021 aagaaggaaa caactatgat caaattaaaa cgtgtttacc ttgatgctga aaaagaggat
    16081 ggctatagaa tactcgtgga tagattctgg ccacgaggaa tttcaaaaga aaaagcgcag
    16141 ttagatctgt ggttgaaaga aattgcacct agtaatgaag cacgcaaaac ttttgaccat
    16201 gtaccagaac gatttgcttc atttgcaaag aaataccagc aagaattgat tttcgaagaa
    16261 gaaaagaagc aagctatttt taaactcagt caacttatga aagaacatcc agtcattact
    16321 tttgtttatg gtgcaaaaaa tgaacatcag aaccatgctg ttttattgaa aaaatttatc
    16381 gaaatggagg catgcaaatg aaaataggga tcattggtgc cggacctaga ggattaagtg
    16441 tagcagaaag gattctccat aataatcagg acagacattc tcttgaactt tttcttttcg
    16501 atcccaatgg tcctggaggt cgagtttggc ggttgaatca gccaccggag ttattaatga
    16561 attctgtttc tcaacaagtg acgcttttta cagatgaaac attgtccagt ggaggattgg
    16621 taacacaggg acccaacttg tttcaatgga gtaaaggacc tgctacagat tatatcgaac
    16681 agacggtaac caaaaataag caactaatta tagaagaaat caatcgtctg gaaaaagatc
    16741 agcaatctac tcgatgttta tacggactct atcaacgctg gtttttctca aagttgcaag
    16801 aagaatttcc agcttctatt cagtttgttt attctttagt aaaaaaagta agcaaaagaa
    16861 aagatggtta tcttttacag acagatcagc aaagctattc tatcgatcag cttgttttag
    16921 caagcggtca ctgggaaaat gatgagacag aagaagaaag agtgtgggta gactacgcaa
    16981 agaaaaatgt tttactgtat cagcttcctt ctaatcctgc agatgtagat ctttcgaaga
    17041 taccgcctaa agaaaatgtc tttttaagag gtctaggctt aagttttttt gattatgtcg
    17101 gattgtttac agtatcaaga ggagggcgct tcgagaacga aaagggaaaa ctggtctatc
    17161 atccatcagg caaggaaccg attgtataca gtggatcaag aagggggctc ccttattacc
    17221 ctcgaggaag aaatcaaaag caaggcggag caatggcatg gccacggtta ctaacaaatg
    17281 aaaacctgga gcaatggcat cagtcgggat ttttgactgg cgaaacattt tttgactatc
    17341 tgaaaaaaga cgttgagttg ttttattaca agaaaatcat cgaagaatat cacttgccga
    17401 ttccaaagaa aaattttgag catgattttc tctccctctc ttcagaggag tgtctcggaa
    17461 agcatccagc actaatccct tatagatggg attggtcgta tttagaaact cctcttgcct
    17521 atcagaatga gcccttttca gaagctagcc gaatatttat tgaagggcag ataaaagaag
    17581 cggaaaaagg caattgcaca ggagcatttg cttcaacatt tgatgcatta aaagactggc
    17641 gtgatccaat ccggcaggct attgaatggc acgttttttc tgctaaggaa tacaaagaaa
    17701 tactatgggg atggtttact catctaaata gctttttaac gatcggtccg ccaatcattc
    17761 ggacgaaaga attagcagca ctgatagatg cgggtatttt tcatttgatc gaaccgccat
    17821 ttgatattca gcaaaagcac aaccgctatg ttatttctct acaagataaa gaaatcagca
    17881 gtcgctattt gattgaagct cgattgccac aaacaagctt ttctcgttca agtaatcctg
    17941 tcataaaaaa tttgaaggaa aacaaacttg ttcgcccttt ttcattttca gataatacag
    18001 gaagttatca ttcagaagct atagatgttg agcgaggtac gaatcgcgtg attgatgctt
    18061 caggggaaat ccagtctgac ttttattgct atggaatacc tgttgaaggt gtggattggc
    18121 taactgcagc agtagctaga ccttatacag atccatggaa tctgagacag gcagataaaa
    18181 tagcacaact tatattgact gacaaataaa aatacgcatg atgacttatc tgttttttag
    18241 aaagtcttca tgcgtatttt taaataatta ttaacgagaa acgcgtttac gcattgcttt
    18301 tttacggttt tcttcgatca ttgcttcttt ttcgtcaatt ggatcgatca ctgttttttt
    18361 cacagaagca accaaaccag caacggcagc ggctgttgcc agacttccta ctaatattcc
    18421 agatacaaat tttttcattg aatccacctc atttattatt tgttatatct attatggaac
    18481 aaaagagaaa aaaaggctag ttttacgttt gtttgtaaac ctttgcaaaa aatc
//
LOCUS       DAZKLM010000057        18488 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3764
            SAMEA115706913-rid22582563.denovo.058, whole genome shotgun
            sequence.
ACCESSION   DAZKLM010000057 DAZKLM010000000
VERSION     DAZKLM010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706913
            Sequence Read Archive: ERR13196647
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18488)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18488)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287823.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287823.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,004
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,849
            CDSs (with protein)               :: 2,849
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 41 of 96
            Pseudo Genes (incomplete)         :: 52 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 15 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18488
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706913-rid22582563.denovo.058"
                     /isolate="VRE3764"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(112..657)
                     /locus_tag="ACG842_001795"
     CDS             complement(112..657)
                     /locus_tag="ACG842_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase family protein"
                     /protein_id="HFQ0248333.1"
                     /translation="MNALISIDYTYDFVAEDGKLTTGTAGQVIEKKLTAHTKKFIDQK
                     DFVVFAIDAHDPKDSFHPENRLFPPHNVIGTGGRELYGSLKSLYQDYEQEENVYWIDK
                     RHYSAFSGTDLDIRLRERQITDIYLTGVCTDICVLHTAVDAYNLGYKLHIFKDSVASF
                     DPVGHEWALRHFESTLGAEIL"
     gene            complement(669..1361)
                     /locus_tag="ACG842_001796"
     CDS             complement(669..1361)
                     /locus_tag="ACG842_001796"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008782 - adenosylhomocysteine
                     nucleosidase activity [Evidence IEA];
                     GO_function: GO:0008930 - methylthioadenosine nucleosidase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009164 - nucleoside catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HFQ0248334.1"
                     /translation="MKIGIIGAMEEEVKILRENLSEPLSWERAGALFISGSLGNHEVI
                     VVRSGIGKVLASITTSLLIQQYGVNMVINTGSAGGIGEGLQVGDIVISDKVAYFDADA
                     TGFGYNPGQLPGMPLYYEASTYLRSEMVKAAKATNLNAKEGLIVTGDTFVDSPDKIKE
                     ILTNFPEALACEMEGAAVGQTARQFNIPFLIVRAMSDTADHSATQSFDEFIEDAGKRS
                     AEMVIEFVKHLV"
     gene            complement(1380..1664)
                     /gene="macP"
                     /locus_tag="ACG842_001797"
     CDS             complement(1380..1664)
                     /gene="macP"
                     /locus_tag="ACG842_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall synthase accessory phosphoprotein
                     MacP"
                     /protein_id="HFQ0248335.1"
                     /translation="MAKGPLITRSELRKRQQAQASESLKKQRKAETAYQQEEKKIASF
                     YRKESKKNKPITKTRISEREKTTKWNSFLMKSLIIVILMLCVVFLAIAFI"
     gene            complement(1677..2240)
                     /locus_tag="ACG842_001798"
     CDS             complement(1677..2240)
                     /locus_tag="ACG842_001798"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HFQ0248336.1"
                     /translation="MLNNEQFEEKTISRKEIYSGKIIDVAVDEVRLPDGGTSKRELVF
                     HPGGVGIIAFDEQDRLLLVKQFRKPLEKVILEIPAGKIDPGEGQNPEMTAARELEEET
                     GYRAKSLSHLTSMYLSPGFANEVLHIYHAQGVEKVENPLAQDEDEVLELYHLTLEEAQ
                     QAMKDQLICDAKTIYAIQYWELLTKGK"
     gene            2468..2722
                     /locus_tag="ACG842_001799"
                     /pseudo
     CDS             2468..2722
                     /locus_tag="ACG842_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318486.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="5-bromo-4-chloroindolyl phosphate hydrolysis
                     protein"
     gene            2817..3776
                     /locus_tag="ACG842_001800"
     CDS             2817..3776
                     /locus_tag="ACG842_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFQ0248337.1"
                     /translation="MTYNHLTPTELVMIEAYFNQSQPVSKVANLLQRSRQTIYKVYRF
                     LKSGGSAMSYYKQYKKNKRNCGRHPIVLPDEQQEYIQKKVVQGWAPDVLIGRAEFPIA
                     CSVRTLYRMFKDKTFDTHDLPMKGKRKPNGHKEKRGKQGFRRSIRDRKTDYTQFDHEF
                     GHLEGDTIVGGKHKSAVITLVERLSKVIITLKPEGRQAKDIENRLNQWFRCVPKNLFK
                     SITFDCGKEFSNWKKISNTNDISIYFADPGTPSQRGLNEHSNGLLRKDGLYKAMDFNG
                     VSETFIQSVASKRNHIPRKSLHYRTPMEVFLSYVTKDDLSNLI"
     gene            3786..4196
                     /locus_tag="ACG842_001801"
                     /pseudo
     CDS             3786..4196
                     /locus_tag="ACG842_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318486.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="5-bromo-4-chloroindolyl phosphate hydrolysis
                     family protein"
     gene            4320..5516
                     /locus_tag="ACG842_001802"
     CDS             4320..5516
                     /locus_tag="ACG842_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HFQ0248338.1"
                     /translation="MDHKPTETKDVTPVNDTLEDLLNNPFSTPTDTLTTSQQAEIDQL
                     QEQQTAARLIDKLPAERQEQAKQLAAKIDANDAQSVISYGSAAQAKLSEFSQSMLNHV
                     QAQDIGPVGDSLTELMYRLQEANPDELRAGEGNIFQRVFGKVKQSIYEVTAKYQKIGA
                     QIDKISVKLDKEKDGLLKDNLMLEQLYQKNKDYFDALNIYIAAGELKMEELQTTIIPE
                     AMKRAEETGDQMDVQIANDYTQFLDRLDKRTHDLRLARQITIQQAPQIRLIQNTNQAL
                     AEKIQASIATAIPLWKNQVVIALTLLRQKDAVTAQRQVSETTNDLLKKNSEMLKISAI
                     ETAKENERGIVDIETLQTTQNDLIETIQETLRIQKEGKEKRRHAEVELGHMEEDLKQK
                     LLDLTQ"
     gene            complement(5617..6993)
                     /locus_tag="ACG842_001803"
     CDS             complement(5617..6993)
                     /locus_tag="ACG842_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFQ0248339.1"
                     /translation="MDLFRKKEINLNHTSEMKKELKTSDLIMLGIGAIIGTGIFVVTG
                     VAANQNAGPALSLSFVLAAIVVILSGLSFAEFASRVPVIGGPYAYLYVVFGEFAAWLT
                     GWLLIGEFLLAVSSVASGWSGYMQGFLKSLGAELPQALTGGYNPENGTYIDLIAVLVV
                     IFVTYIVSLEAKKALRLNNAMVYVKFGIIALFIIVGIFFVKPDNWQPFMPFGFSGVLD
                     GAALVFFAFLGFDAVAMAAEEVKNPQKDVPRGIIGSILIATVLYIIVTLILTGIVPYT
                     ELGVNDPVAFAMRYVGHGTVGAVIAVGAILTLLTVTISMMYSLARLLFAVSKDGLLPK
                     FMTEIDQKHRTPKKATYAAGVAAVFFAGFFPLNVLAELTNIMALAYLMLVSLGLLKLR
                     KMFGKPKAGEFKVPFVPLLPIVSILTCIVLMLRLQTVTWIVFGIVMVIGLIIYFGYGY
                     GHSKMNEK"
     gene            complement(7030..7896)
                     /locus_tag="ACG842_001804"
     CDS             complement(7030..7896)
                     /locus_tag="ACG842_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GRP family sugar transporter"
                     /protein_id="HFQ0248340.1"
                     /translation="MEILIALVPMLAWGSIGLVSGKIGGDANQQTLGMTIGAFLFSLV
                     VFFVVSPVITPWIFLIGFLSGLAWSVGQNGQFHGMKYMGVSVGLPLSTGFQLILNTIA
                     GAVFFHEWTQTKDYVYGIIALALLVSGAYLTARQDDEGKIDTDNKMLDFGKGFRALIF
                     STIGYGVYTIIVNWANLDAMSIILPQSIGMILGASFFAFRKVKVDQFVWKNMICGLLW
                     GLGNICMLLTVKSLGLAVGFSLSQMGIIISTLGGIFILGERKTKKELIYVIVGCLLVI
                     LGGILLGYMKTV"
     gene            complement(7985..9010)
                     /locus_tag="ACG842_001805"
     CDS             complement(7985..9010)
                     /locus_tag="ACG842_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFQ0248341.1"
                     /translation="MNFFKYLKDQWTLILGWLFFIALTIFVMWLAPNIKVDLSTIAYL
                     ALIEGVFLIFYLLGGYLSKRRWWGKLADIQKTSPLQNYLSGGRTEEEKLVEEYINQLI
                     REHQEVMQSAISNQQDQKDYIDSWVHEIKVPLSASKLLVRSIEFDIDDQKYTLLENEL
                     SKIDEYVEQVLYYARLDSFSRDYLIQEYGLKGIIQPVIRSQANYFIQKNIRYEIVGED
                     HTVLTDAKWIAFIFNQLLSNAIKYTPDHGNIIVSIEKEAQGVSLSVKDSGIGIPAEDL
                     KRIFDKGFTGRNGRLSKTHSTGLGLYLAKNLAEKLGIHLTAESTEGKGTTMTLFFPIL
                     NFYNEKR"
     gene            complement(9028..9702)
                     /locus_tag="ACG842_001806"
     CDS             complement(9028..9702)
                     /locus_tag="ACG842_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFQ0248342.1"
                     /translation="MAKIMVVEDEEIIRQLIMEELEKWQFETFGTTDFNQVFSDFERE
                     EPQLVLLDINLPVLDGYYWCQKIREVSKVPIIFISSRNTNMDMIMAMNMGADDFVTKH
                     FQIDVLIAKINALLRRSYNYTELSSEMMSHNGITLNVDNGSMEINGEIIDLSKNEYRL
                     LFILMKHNGKILSREKLLRALWDDERFVDDNTLTVNINRLRRKIEQAGIQGYIETKVG
                     QGYIVP"
     gene            complement(9886..10779)
                     /locus_tag="ACG842_001807"
     CDS             complement(9886..10779)
                     /locus_tag="ACG842_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFQ0248343.1"
                     /translation="MKKKIGNRPQTQISLLLQLFMAVMFLAGAAVFTYPFLADALSNY
                     LDQRRIENYQKQLERDKEEKHEQQLAVHEKKNQTLAHTSVIPGMGQVKDPFEQAVRNV
                     QNPGKEYYEQHMIGAIYIPKINVSLPLFDETNDLLLDRGATVLQGTSFPTGGESTHSV
                     ITAHSGIAEKKLFTDLEEMEQDDRFYLEVYGRMLAYEVVEKIVVLPTKTNTLAIREKQ
                     DLVTLITCTPYTVNTHRLLVTGRRVPFTEEVSSKIEQTKKYHLYRLLILLLGILLVLT
                     LFGYWCYRKFKRQKQRKKNNR"
     gene            complement(10853..12550)
                     /locus_tag="ACG842_001808"
     CDS             complement(10853..12550)
                     /locus_tag="ACG842_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HFQ0248344.1"
                     /translation="MNKKMIVTGMSVLLLVTAGIGIFGNGKLVKAEEVAQKPSEVTIT
                     LHKKGFSSVPEERPNSGLVSTDFGEENIPGVDFDLFDVTEVYYDLIRDNPLTPEREDG
                     LNSAEAIEWIQKRHTESWFLKYRLTSIDKQTTNEAGEAVFSTVQVTEEAPSSRDKVYL
                     FLETYSPAHISRIASPMVVMMPVMMPDMVDGVWDGSTWKDTYNTDVHLYPKNEIREAD
                     KQMNVEESDLRQVTIINEAGEQETISYIDLERGKTASYTITAPIPYFIDSVLENGSAV
                     IKNYKITDTPTVGLTYYDQEIEVRAGETILTKGQDYIVEVVSNGFVVTILTEENGVAK
                     VDTLGRLADARGGDLTITYNLKVSTELEADDFHNNTAVIEIGRNDEFDYEEGVEPPEK
                     VTTGGRKFEKYDASSSELLKDARFELWNEDRSEYAIFYKGESPLAVYESGADRIEWAT
                     SGQATEFVADGNGYFEVQGLDYGTYQMKETMAPEGYVLPTGEAAFTEFIISYGSYNEE
                     IQIVGVENPGPERVPNMKRGSLPATGGNGLLAFLLIGISLMIGAYSWYRKSKMKSEV"
     gene            complement(12543..13856)
                     /locus_tag="ACG842_001809"
     CDS             complement(12543..13856)
                     /locus_tag="ACG842_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilin N-terminal domain-containing protein"
                     /protein_id="HFQ0248345.1"
                     /translation="MKKLLCLMLQLLVLLPLAAVLLPHAMSAEESGEDVVEFVLHKRM
                     IRDIDYNDQFEYHENDGLAISEEAGETADIISQTVPLNGATFAIYDMTDYYHEKKAAE
                     NMTPKEFVQQIAETNRNNIRQLITEERLVQIGTSVTTGKDTVGGLGDGIARIKAPKKN
                     QDRDAVYLIFEESIDPEVGLDVDIDQTAIPIAAILPIYHPTESTEELQEIHIYPKNVG
                     YLRDPYFFKYGRQRGTTEKGKPLEGAVFALYQMIEGGKYYLDMAPANDLKNQWIKPAD
                     NDPLNDKNVSKFISDKEGLVTTGQRFLPSGTYYFEELKGVEGYEMDEASKRIEVIIPE
                     FWVDAKGQPQYVVVNGQEMAELESGKVPVSAYESATPRVYNETVKETTDKPKEPSGPS
                     NPGQSGKWSVFPQTNEARTLISLLGILLLLGAVLLIKKERGEKNE"
     gene            complement(13856..17797)
                     /locus_tag="ACG842_001810"
     CDS             complement(13856..17797)
                     /locus_tag="ACG842_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HFQ0248346.1"
                     /translation="MEKLDEPIVPSGQALQRIQRQLFNGILERLGPTATQDPYEYIYP
                     SGEVENEQHRYPKFTTNDYTKVLGNWTQSNERYQGDKGSPYDGENLIDDSDYTNGGAN
                     WRNYDYATNNGSTAGEEDRNVPAQTVQLWGNERNFENSYLDYNGAYIKKWVEPVLPSS
                     TASDLHPEDATTLYNVYLDVIGGEKKEISPIDIVFVLDKSASMSELTAGTNSQTKNAA
                     LIEAVNEMSKDLLSDPSLDIRIGMVNFYHNSTAINNHEQISSDIFPLTNDINRLTGSE
                     NTALNRTPIGGTPLTLGLKNGYETLYKDNGGENRNPEKILIVVGDGTPTFSYAPIQSS
                     YRTSTNGAWSNWTVMEDKIAEDNDVLFRNFEEFSGNTSNAGFTHPVTYASDFNRPEDE
                     VNVHYRYGEVKEGDNKATHWVGDGSSNNNTNGSPTSQEKSSAINTVAYHHWLKNKYQE
                     NPPSIFSIGLGIDGNVSGRQRLDAIGRNVLKNIADLEEDGVTPRYYNANNKNDIVTAL
                     EDISSTFKRTIQQATLYDETGFNVSPFGAGRSADIQFYHLDPGENGMKYQEPEIWDEE
                     RHGQAPPEVVATPTTYVGRENQAYKFSPISLGEGEMVRIKYQVKLDAGAQDGNFCTVN
                     NMAYIQNLEDYENDITTGRMYFPSSSIRYEHKGRNFQVSKKGQNGEPLAGVEFALYDQ
                     NPESGEAEPIETSKTTTDGIAIFETKMPILGDETGAVSDIFWIREIAGPPRYQLVDET
                     YQFQIKKYAANEDEMLGHYELVDEYTSGSRVGGPEGKDPNSYYEGEYHKLSVVQEPNI
                     EGTEYDELYVKLEMRNEFKPIHMNINKLIEGSNMPINGAEFELYETDGNGLEIGYPVA
                     KGLSGVNEVEEEKGRLTFYSVDEDGKYVIVDGEKVIHPIGEPDSFVYKDSEETTNYAE
                     YKIIESQNPEGFKEPDENDTWILRLYDNAIGTIQLKQTGEENWQYLTYTTDEEDRLWV
                     AFTAYNTFDYREIRVRKLDHEGHTLDGAGFDIRKVGDSSFPLYRAYTGNPLEPEESKR
                     LPGIGYFYSRAVGVEKYVDLNYDQPLHLSIGEYEVSESVAPNGYRLSDQVFQFALNEE
                     GKFIIEDTVIEDETAPLPEGYEIIDGVLQVTLEDELAPIDLELLKIDSMNSRRLEGAE
                     FSIEKQNSAGEYEIIEGGLAPDPNDSSLFNASDLLEGVYRIKEVKSPDSYRKLPGYFI
                     LEISYREEPDIENDRVVPGKEAGTLKVEISYYPNEEAETPDLQQELEYELLETNRIRL
                     QLSVGNDPENPLPATGGSGRLFYILLAAFFMALAGAAYGLFVRQSKKEGAR"
     gene            complement(17906..18364)
                     /locus_tag="ACG842_001811"
     CDS             complement(17906..18364)
                     /locus_tag="ACG842_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFQ0248347.1"
                     /translation="MKGLKLKLLQLVTVSLLFGPYFQSVYHMTTLDVAAESTETKEDE
                     VFLDEDYAKIEGKYIEKEETLEWHLAFEKKESANEGRIRLAIDAAAAGIGTVKNVRGT
                     GLSSYDEDGEDLKVETIDGQEWYVGKVYSKEAETGTLIFETEKIPDVNEG"
BASE COUNT         5281 a         4095 c         3221 g         5891 t
ORIGIN      
        1 aataagtttt tttaaaataa cgtataaaaa agagcataaa acaaagtcgt ttgttttatg
       61 ctcttctttt attattttgt gctttttatt ttatcaaatc actttgtttt tttataaaat
      121 ttctgcgcct agtgtggact caaaatgtct caatgcccat tcatgtccaa cgggatcgaa
      181 acttgccacg gaatctttga aaatatggag tttgtatcct agattatacg catcgactgc
      241 tgtatgcaac acacagatat ctgtacatac gccggtcaaa taaatatctg tgatctggcg
      301 ttctcgcaaa cggatgtcta gatctgttcc actaaaagcg gaataatgcc gtttgtctat
      361 ccaataaaca ttttcctctt gctcatagtc ttggtataaa ctttttaaag agccgtacaa
      421 ttcccgtcca cccgttccta tcacattatg cggaggaaat aagcggttct ccggatgaaa
      481 ggaatctttt ggatcatgcg catcaatggc aaaaacgaca aaatcctttt gatcaataaa
      541 cttctttgta tgagcagtca gctttttttc aattacttgt cctgctgtcc cagtagtcag
      601 ctttccatcc tctgcaacaa aatcatatgt gtagtcaatc gagatcaatg cgttcatctt
      661 agtcctcctt atacgagatg tttgacaaat tcgatcacca tttctgctga tcgtttgcct
      721 gcgtcttcga taaattcgtc aaagctttgt gtcgccgagt gatctgctgt gtcgctcatt
      781 gcacgtacga tcaaaaatgg gatattgaac tgacgagctg tttgtccaac agcggctcct
      841 tccatttcgc aagctaatgc ttcagggaaa ttcgttaaga tttctttgat tttatctggc
      901 gagtcgacaa atgtatcacc tgtaacgatc aatccttctt ttgcgttcaa attcgttgct
      961 tttgctgctt taaccatttc actgcgcaaa tatgtgcttg cttcataata tagaggcatt
     1021 cctggtaatt gtcctgggtt gtagccaaat cctgttgcat ctgcatcaaa ataagctact
     1081 ttgtctgata taacgatgtc acctacttgt aagccttcac cgattccgcc agctgaacct
     1141 gtattgatca ccatgttcac gccgtattgt tggatcaaca gacttgtggt aatagatgct
     1201 aagacttttc ctataccgga acgtacgacg atcacttcgt ggttacctaa tgaacctgaa
     1261 ataaataaag cgcctgcgcg ttcccatgat aatggttcgc ttaggttctc tctcaaaatt
     1321 tttacttctt cttccattgc gccgataata ccgattttca tctttttaat tccttctttc
     1381 tatataaatg cgattgctaa aaaaaccaca cataacatta ggataacaat aataagagat
     1441 ttcatcaaaa aagagttcca tttggtcgtc ttttcccgtt cgctgatcct tgttttcgtg
     1501 attggtttat tctttttgct ttctttacga taaaagctgg caatcttttt ttcttcttgc
     1561 tgataagctg tttctgcttt tcgctgtttt tttaatgact cgcttgcttg tgcttgttgt
     1621 cgcttgcgaa gttcgcttcg cgtaataagc ggtcctttgg ccataagccg atccctctac
     1681 tttcctttgg tcaatagttc ccagtattga atcgcataaa tcgtctttgc atcacaaatc
     1741 aactgatctt tcatcgcttg ttgcgcctct tcaagagtta gatgatacaa ctccagtact
     1801 tcgtcttcat cttgtgctaa aggattctcg accttctcta ccccttgtgc atgataaata
     1861 tgaaggactt catttgcaaa acctggtgaa agatacatcg aagtaagatg gctcagcgac
     1921 ttcgcacgat agccagtttc ttcttctaat tctctagctg ctgtcatttc gggattttgt
     1981 ccttctcctg gatcgatttt tccagctgga atctccaaaa tcactttttc taaaggtttt
     2041 cgaaattgtt tgacaagaag cagacgatct tgttcgtcaa aagcaatgat cccgacaccg
     2101 ccaggatgaa ataccagttc tctcttcgaa gttcctccat ctggcaaacg aacttcgtct
     2161 acagcaacat caataatttt tccgctatag atttctttgc gactgattgt tttttcttca
     2221 aattgctcat tgttcagcat atcttctcct gctttctttg taatcataac tgcttttttt
     2281 tcatttttca atttttctca aaaactcctt attagtttac catagataaa ccttagattt
     2341 tctctaaata tagaaaatcc ttctttagtc cgatactctt tctagtagtc cgtatgctaa
     2401 aataagtatg taatcgaata acgaaaagaa ttcaatactc aacgataaat gacatggagg
     2461 gtttattatg ataaaacgaa attggccgtg gcttctagca ttggtcttgc tatttctctt
     2521 tgtccgaaac aatagttttt cttttgttct aggactttta ttgataggtg tagttttagt
     2581 tttgtgggga ctctttggtt cgcgcaggaa aaagggaaaa caagaagaaa ttcctgcact
     2641 gactgacgaa cttgaaactc actatgaaaa aagcggtatg acagcgaatg aaatttcatt
     2701 ttttcgacaa acaatgaatc aacgccgatt gtaaaattaa gctagacaac taaaaaaagt
     2761 catttgtgct acactcaaaa tagttccgac gaaagaatta taaggagtga gtacaaatga
     2821 cctataacca tcttacacca actgaactcg taatgataga agcttatttc aatcaatcac
     2881 aacctgtttc aaaagtagcc aatctgttac aacgttctcg acaaactatt tataaggtct
     2941 atcgttttct taaatctggt ggaagcgcaa tgagctacta taaacaatat aagaaaaaca
     3001 aacgtaactg cggaagacat ccgattgttt tacctgatga gcaacaggaa tacattcaaa
     3061 aaaaggttgt tcaagggtgg gctcctgatg tccttattgg tcgagcagag tttcctattg
     3121 cttgttcggt acgcacactg tatcgaatgt ttaaagataa aacctttgac actcacgatt
     3181 taccaatgaa gggaaaaaga aaaccaaacg gtcataaaga aaaaagagga aaacaagggt
     3241 ttcgccggtc aatccgagac agaaagacag actataccca gtttgatcat gaatttggtc
     3301 acctggaagg tgacactatc gtgggtggaa aacataaaag cgcggtcatc acattagttg
     3361 aacggctatc taaagtcatc attaccttga aacccgaagg cagacaggct aaagacattg
     3421 aaaataggct gaatcaatgg tttcgttgtg tcccaaaaaa cttatttaaa tccatcacat
     3481 ttgattgtgg aaaagaattc tcaaattgga aaaagattag taataccaac gatatttcaa
     3541 tttattttgc ggatccagga acaccttctc aacgaggatt gaatgaacat tcaaatggct
     3601 tattacgtaa agatggtttg tataaagcta tggattttaa tggtgtatct gaaaccttta
     3661 tccaatcggt ggcatcgaaa cgaaatcata ttcctagaaa atcattacac tatcggacac
     3721 caatggaagt atttttgagt tacgtcacaa aagatgattt gtctaactta atttgacaaa
     3781 cgaaacaaac aaaaaatgag atcaatcaat tgcaaatgaa tatgcagcag acagccaaac
     3841 ttaaagcaat cgatctccgc catgatccag tcaaagcagc gaaagcttta ttcaaagaat
     3901 tggtgaaaga accaaaccga ctgcatgaag caagtcagtt tctttatacg catctgccga
     3961 atctagttga tttgacaaat aagtataatg agatcaatga ccatgagatc aaaaacaaac
     4021 agacctatga aaaactagaa gaaagcgcgc agatcatcga ccagctgtca gctttaatcg
     4081 cacaagatta tcaaaaattc gttgctgagg atctagatga tatcgacgta gagatatctg
     4141 ttgcgaaaca gagcttaaag cgtgataata aatatgaacc agaacaaaag gattaactct
     4201 tttgttcata aaagaagact cggaacattc agatgatgtc tggctgtttc gagtactttt
     4261 tctaaagtag agacattcat gtaagaatcg agaacagatt caacaaggag gaatttttta
     4321 tggatcataa accgactgaa acaaaagatg ttacaccagt taacgataca cttgaagatt
     4381 tattgaacaa cccgttttca actccaacag atacgctgac cacttcacaa caagcggaga
     4441 ttgatcagct ccaagagcaa cagacagctg ctcgtttaat tgacaaacta ccggcggaac
     4501 gccaagaaca agctaaacag ctagcagcaa aaatcgatgc taatgatgca cagtctgtca
     4561 tcagctacgg atcggctgcc caagcaaaat taagtgaatt ctcacaatcg atgttgaatc
     4621 atgtgcaagc acaagatatt ggtcctgtag gagattcctt aactgaactg atgtatcgtc
     4681 tccaagaagc caatcctgac gaactgcgtg ccggagaagg gaacatcttc cagcgtgtct
     4741 tcgggaaggt caaacaatcg atctatgaag tgacagcaaa atatcaaaag attggtgcac
     4801 aaattgacaa gatttcagtg aaattagata aagaaaaaga cggtctgcta aaagataatc
     4861 tgatgttaga acagctttac cagaaaaaca aagactactt tgatgcactg aatatttata
     4921 tcgctgctgg agaattgaaa atggaagaac ttcaaacaac gatcattcca gaagcgatga
     4981 aaagagcaga agaaactggc gatcagatgg atgttcagat tgccaatgat tacacgcaat
     5041 tcctagatcg tctggataaa cgtacgcatg atcttcgctt agctagacag atcacgatcc
     5101 agcaagcacc acaaatccgt ttgatccaaa acacaaatca ggccttggct gaaaagatcc
     5161 aagcatctat cgctacagcc attccgctgt ggaaaaacca agtcgtgatc gcattgacct
     5221 tgcttcgtca aaaagatgca gtaactgctc aacgccaagt ctctgaaaca acaaacgact
     5281 tattgaagaa aaattcagaa atgctgaaaa tctcagccat cgaaacagcg aaagaaaatg
     5341 aacgtggaat cgtggatatc gagacccttc aaaccactca aaatgatttg atcgaaacaa
     5401 tccaagagac attgcgtatc caaaaagaag gaaaagaaaa acgtcgtcat gcagaagtag
     5461 agcttggaca catggaagaa gatctgaaac aaaaactgtt ggatttaacc caatagatca
     5521 cctttcgctt gttactaaaa caaacgccca acaacagttt attacaactg ttgatgggcg
     5581 tttttcttat ctttcttcta acttctccat tggagtctat ttctcattca tcttgctgtg
     5641 tccatagcca tagccaaaat aaatgatcag tccaataacc attacgatac cgaacacgat
     5701 ccatgtcaca gtttgcagac gaagcatcaa tacaatacaa gtcaagatgg agacgattgg
     5761 caatagcgga acgaatggca ctttgaattc tccagctttt ggtttgccaa acatttttct
     5821 caacttcagt aagcctagac taacaagcat caaataggcg agcgccatga tattcgttag
     5881 ttccgctaat acattcaatg ggaaaaatcc ggcaaagaat acagctgcca caccagcggc
     5941 ataagtcgct tttttcggtg tacgatgctt ttgatcgatt tctgtcataa acttaggaag
     6001 taagccatcc ttgctgacag caaacagtaa acgagcaagt gaatacatca tcgagatcgt
     6061 taccgtcagt agcgttaaaa ttgcccctac agcgatcacc gcgcccactg ttccatgtcc
     6121 tacatacctc attgcaaaag ccactgggtc attgactcct agttcagtat acggaacgat
     6181 tcctgtcaag atcagcgtca cgatgatgta aaggacagta gcaatcagta tcgaaccaat
     6241 gattcctctt ggcacatctt tttgcgggtt tttgacttcc tcggctgcca ttgcaacggc
     6301 atcaaatccc aagaaagcaa aaaacacaag agctgcacca tctagcacac cggaaaaacc
     6361 aaaaggcatg aatggctgcc agttatcagg tttaacgaaa aatattccta caatgataaa
     6421 caacgcgatg ataccaaatt tgacatatac catggcatta ttcaaacgta atgctttctt
     6481 ggcttcaagc gacacgatgt aagtgacgaa tatcactacg agcaccgcaa tcaagtcaat
     6541 gtatgtacca ttttccggat tgtacccgcc agttaatgct tgtggaagtt cggcccctaa
     6601 acttttcaaa aaaccttgca tatagccaga ccatccggaa gcaacagagg aaaccgctag
     6661 taaaaattcc ccgatcaaca accaccctgt cagccaggca gcaaattcac caaatacaac
     6721 atacagatag gcatatggcc caccaatcac tggcactcgc gaagcaaatt ctgcgaaact
     6781 tagtccagac aaaatgacaa cgattgcagc taagacaaat gaaagtgaca gtgctggacc
     6841 agcattttga ttggccgcaa cccctgtcac cacaaatatt cccgtaccga tgatcgcacc
     6901 aattccaagc atgatcaaat cagacgtttt tagttctttt ttcatctcac tcgtatgatt
     6961 gagattgatc tctttcttac gaaacaaatc cattgaagtt cctccccatt ttaggttgga
     7021 aaacagcgat taaactgttt tcatataccc taaaaggata ccacctaaaa tgactaaaag
     7081 gcaaccaaca ataacataaa tgagctcttt tttcgttttt ctttcgccaa ggatgaaaat
     7141 cccacctaaa gtagagataa tgatccccat ttgtgaaagt gagaagccaa cagctaaacc
     7201 aagtgatttg actgttagaa gcatgcagat gtttcctaag ccccataata atccacagat
     7261 catgtttttc cacacaaact gatcgacttt aactttacgg aaagcaaaga agcttgcgcc
     7321 taagatcatt ccgatacttt gaggtaagat aattgacatc gcatccaaat tcgcccagtt
     7381 gacgatgatg gtatatacac cataaccaat cgtagagaag atcaatgctc taaaaccctt
     7441 accaaaatca agcattttat tatctgtatc tatttttcct tcgtcatctt gtcgggcagt
     7501 caaatacgca ccgcttacaa gaagagctaa agcaataata ccgtatacat aatctttcgt
     7561 ttgtgtccat tcatggaaga aaacggctcc cgcaatcgta ttcaaaatta attgaaaacc
     7621 agtagacaat ggtagcccta cagaaacacc catgtacttc attccatgga actgtccatt
     7681 ttgtccaaca ctccaagcta agccggataa aaatccgatc aagaaaatcc aaggggtgat
     7741 tacaggacta acaacgaaaa agacaactaa agaaaacaga aatgcaccga tcgtcattcc
     7801 tagtgtttgt tgattggcat cgccaccaat tttaccactt actaaaccaa tactacccca
     7861 tgccaacata gggactaatg ctattaaaat ttccataacc aatctccttt tatttcacat
     7921 gataagaaac ttatcagcga aaacgttaac aacacaagca ctttttttcg tttctaacga
     7981 ttttctaacg tttttcatta taaaaattta agattgggaa aaataaagtc attgttgtcc
     8041 cttttccttc agttgattca gccgtcaaat ggatacctaa tttttcggcc agattttttg
     8101 ctaaataaag tcctaatcct gttgaatgtg ttttgctaag tcgaccattt ctcccagtaa
     8161 atcccttatc aaagatccgt ttcaagtctt ccgctgggat accgatccct gaatctttaa
     8221 ctgaaaggct gactccttgc gcttcctttt ctatgctcac aatgatgttt ccgtgatccg
     8281 gcgtatattt gatggcatta ctaagcaatt ggttgaagat aaaagcgatc catttagcat
     8341 cggtcaaaac agtatgatcc tcaccaacga tctcgtagcg aatatttttt tggataaaat
     8401 aatttgcctg actgcgtatc acaggctgaa tgattccttt tagtccatat tcttggatga
     8461 ggtagtcccg cgagaaactg tcgagtcttg cataatacaa aacttgttct acgtattcat
     8521 cgatttttga taattcattt tctagtaatg tatacttttg atcgtcaata tcgaactcga
     8581 tcgaacgaac tagcaattta gaagcggaaa gtggcacttt gatttcatgg acccatgagt
     8641 caatataatc tttttggtct tgttgattac tgattgctga ttgcattact tcttggtgtt
     8701 cacggatcag ctgattgata tattcttcta ccagtttttc ttcttctgtc ctcccaccac
     8761 tcaaataatt ttgcaaaggg gaagtttttt gtatgtcggc taattttccc caccatcttc
     8821 tcttgctcaa gtaaccaccc agcagataaa aaatcagaaa gacgccctca atcaaagcta
     8881 aataagcaat tgtgctcaaa tcaactttga tgtttggcgc cagccacatc acaaagatcg
     8941 tcaatgcaat aaaaaacagc catcccaaga tcaatgtcca ctgatctttc aaatatttaa
     9001 aaaaattcat cttaaccgct catcctttca gggaacgata tatccctgtc ctacttttgt
     9061 ttcgatatat ccttggatac ctgcctgttc gatttttcgg cgtaaccgat tgatgttgac
     9121 tgtcaatgta ttgtcatcta caaatcgttc gtcatcccac agtgctcgca gcaatttttc
     9181 ccgacttaag atctttccat tgtgcttcat caaaataaat aatagacgat attcattttt
     9241 gctcaaatca atgatctctc cgttgatttc catacttccg ttgtcaacat tcaacgtaat
     9301 gccattgtgg ctcatcattt cactagataa ttctgtgtag ttgtaagaac ggcgaagtaa
     9361 tgcattgatt tttgcaatca gtacatcgat ttggaaatgt tttgtcacaa aatcatctgc
     9421 ccccatgttc atagccatga tcatatccat attcgtattc ctgctggaga taaaaatgat
     9481 cggtactttc gatacttcac gtattttttg acaccaataa tagccatcca aaacgggaag
     9541 attaatatct agtaatacaa gctgtggttc ttctctttca aagtctgaga acacttgatt
     9601 gaaatcggtc gttccaaagg tttcaaattg ccatttctca agttcctcca tgatcaattg
     9661 tcgaatgatc tcttcgtctt cgactaccat gatttttgcc ataaaaaatc cctccatctt
     9721 tcttcttgct actacaatat caaagaagcc cttgtgaaac aagttggctt acctggaaaa
     9781 tgtcatttta gcaaacgtta aaaaaaactg gaaatatgcg atttaatcgc gtttgtctct
     9841 tccaagcagc ttaaatttat tatgttattc ttaataattg gtctattatc tgttattttt
     9901 ctttcgttgt ttttgtcttt taaattttcg ataacaccaa taaccgaaaa gcgttagcac
     9961 tagcaatata cctagaagga gtataagtaa tcgataaaga tggtattttt ttgtctgttc
    10021 tatctttgaa gagacttctt ctgtaaaggg aactcgtctt cctgtcacta gtaatcggtg
    10081 agtgttcacg gtgtaaggtg tacaagtgat aagtgtgact agatcctgct tttctctaat
    10141 tgctaatgta tttgtttttg ttggaaggac aacaattttc tcaacgactt cataagcgag
    10201 catccgacca tacacttcaa gatagaatcg gtcatcttgc tccatttctt caagatccgt
    10261 aaagagtttt ttctcagcaa taccgctatg ggcggtaatc acggaatgtg tactctctcc
    10321 tccagttgga aaagaggtgc cttgcaggac tgtcgctcct ctatctaaca aaagatcatt
    10381 cgtttcatca aataaaggca agctaacatt gattttcgga atatagatgg ctccgatcat
    10441 atgttgttcg taatattctt ttcctggatt ttggacattt ctaaccgctt gctcaaaagg
    10501 atctttgact tgtcccattc ctgggattac tgatgtatga gcaagcgtct gatttttctt
    10561 ttcatgcact gctaattgtt gctcatgttt ttcttcttta tcgcgttcaa gctgcttttg
    10621 ataattctcg atccttcttt gatccaaata attgctgaga gcatctgcta aaaatggata
    10681 tgtaaaaact gcagctccgg caagaaacat cactgccata aataattgta ataaaagact
    10741 gatttgggtt tgaggtctat tacctatttt ctttttcatt ttcttcacct tcttatttaa
    10801 aaaaactgca gcaaccttta taaaaccgat tgctgcagct tatttttgtt gcttatactt
    10861 cagacttcat tttagattta cggtaccagc tgtacgctcc aatcatcaaa cttattccga
    10921 ttagtaagaa tgccaagagc ccgttcccac cagtagcagg aagtgacccg cgtttcatat
    10981 tcggtactct ttctggccct ggattttcta ctcctacgat ctgaatttct tcattatagc
    11041 ttccataaga gatgataaat tcagtaaagg ctgcctcacc tgttggaagt acataacctt
    11101 ctggcgccat ggtctctttc atttgatagg ttccataatc taacccttga acctcaaagt
    11161 atccatttcc atctgcgaca aattctgttg cttgtccgct tgttgcccac tcaattctat
    11221 ctgctcctga ttcataaaca gctaaaggcg actccccttt ataaaatatt gcgtattcag
    11281 aacggtcttc attccacaat tcgaatcttg catctttaag caattcactg cttgatgcat
    11341 catatttttc aaactttctt ccacctgttg tgactttctc tggcggctct actccttctt
    11401 cataatcaaa ttcatcattt cgtccgattt caataaccgc tgtgttgtta tggaaatcat
    11461 ccgcttctag ttctgtggaa accttcaaat tatacgtgat cgttaaatct cctccacgtg
    11521 catctgctaa tctacctaaa gtatctactt tggctactcc attttcttct gtcaaaatcg
    11581 tcacgacaaa cccgttgctt actacttcta ctatataatc ttgtcctttt gttaaaatcg
    11641 tctcgccggc tcgcacttct atttcctgat cataataagt tagaccaact gtaggcgtat
    11701 ctgtgatttt gtaatttttg ataacagcgc ttccgttttc aaggacagaa tcgatgaaat
    11761 aaggaatagg tgctgtgatc gtgtaagaag ccgtttttcc tctttctaga tcaatataag
    11821 aaatcgtttc ttgttctcca gcttcattaa taatcgttac ttgtctaagg tcgctttcct
    11881 ctacattcat ctgtttatct gcttctctga tttcgttttt cggatacaaa tgcacatctg
    11941 tattgtatgt gtctttccat gtagaaccat cccacacgcc atccaccata tcaggcatca
    12001 tcactggcat cataacaacc attggagacg cgattctgga aatatgagct ggcgaataag
    12061 tttctaagaa aagatagact ttatctctag aagaaggtgc ttcttccgtc acttggacgg
    12121 tagagaatac cgcttctcca gcttcattcg ttgtttgttt gtcaatactt gtcagtcgat
    12181 attttaaaaa ccaactttct gtgtgccgtt tttgaatcca ttcaatcgct tcggcagaat
    12241 taagaccgtc ttctcgttcc ggtgttaaag gattatctcg aatcaaatcg taatatactt
    12301 ccgttacgtc aaacaaatca aaatcgactc ctggaatatt ttcttcacca aaatccgtag
    12361 aaaccaatcc gctattcggt cgttcttccg gaacagatga gaaacctttt ttgtgtaatg
    12421 taatcgtcac ttcacttggt ttttgtgcga cttcttcggc tttgactagt tttccgttcc
    12481 cgaaaatccc tattcccgca gtaacgagta acagtacact cattcccgtg acaatcattt
    12541 ttttattcat ttttttctcc ccgttctttt tttattaata aaacagcacc taacaataaa
    12601 agaattccta gtaaagaaat caacgttctt gcttcattcg tttgcgggaa tacggaccat
    12661 tttcctgatt gacctggatt gcttggacca ctcggctctt tcggtttgtc cgttgtttct
    12721 tttacggtct cgttataaac acgtggtgtt gcggattcat aggcagagac tggaactttc
    12781 ccggattcta attcagccat ctcctgtccg ttaactacta catattgtgg ttggcctttt
    12841 gcatccaccc aaaattcagg gatgatgacc tcgattctct tacttgcttc atccatctcg
    12901 taaccttcca caccttttaa ttcttcaaaa taataggtgc cagaaggcaa gaaacgttgt
    12961 cctgtcgtaa caaggccctc tttatccgaa atgaatttgc ttacattttt gtcattcaaa
    13021 ggatcattgt ctgctggttt gatccattga tttttcaagt cattagcagg agccatatct
    13081 aaataatatt tccccccttc tatcatctga tatagagcaa aaacagcacc ttctaatggt
    13141 tttccttttt cagttgttcc tctttgacgt ccatacttga agaagtatgg atctcttaga
    13201 taaccgacat ttttaggata gatatgaatc tcttgcagtt cctcggttga ttctgtcggg
    13261 tgataaattg gcaaaatagc agcaattggg atagctgtct gatcgatatc aacatccaat
    13321 ccaacttctg gatcaatcga ttcttcgaaa atcaaataaa ctgcatcccg atcttggttt
    13381 ttcttaggtg ctttgatacg tgcaatcccg tctcctaatc cgccaaccgt atctttccca
    13441 gttgttaccg aagtccctat ttggacaagc ctttcttctg tgattagctg acggatattg
    13501 tttctgttcg tttcagcgat ttgctggacg aattctttag gagtcatgtt ttcagcagct
    13561 tttttttcat gataataatc ggtcatgtcg taaatggcaa atgtcgctcc atttagtggc
    13621 accgtctgtg aaatgatatc tgctgtttcc cctgcttcct cagagatagc caagccatca
    13681 ttttcatggt attcaaattg atcgttataa tcgatatctc gaatcatccg tttatgcaac
    13741 acgaattcca cgacatcttc ccctgattct tctgccgaca tcgcatgtgg aagaaggact
    13801 gctgcaagcg gtagtaaaac aagtaattga agcatcaaac acaacagctt tttcattacc
    13861 ttgctccctc ctttttagat tgtctgacaa acaaaccata ggctgctcct gcaagcgcca
    13921 tgaagaatgc agcaagcagg atatagaata atcgtccgga tcctcctgta gcaggcaacg
    13981 gattttcagg atcgttcccg acactaagct gtaatctgat tcgatttgtc tccaaaagtt
    14041 catattctaa ctcttgctgt agatcaggcg tctctgcttc ttcgtttgga taatacgaaa
    14101 tctcaacttt tagtgtaccc gcttcttttc ctggaactac ccgatcattt tctatgtcag
    14161 gttcttctcg ataactaatt tctaaaataa agtaacctgg aagttttcta tagctgtcag
    14221 gtgacttcac ttccttgatc cgatagaccc cttccaacaa gtctgaagca ttgaataggg
    14281 aagaatcgtt tggatctggt gccaagcctc cctcgatgat ttcatactct cctgcactat
    14341 tttgtttctc aattgaaaat tctgcgcctt caagacgtcg gctattcata ctgtcaattt
    14401 tcaaaagttc caaatcgatt ggcgctagtt catcttctaa ggttacttgt agtacaccat
    14461 cgatgatttc atagccttcc ggcaagggag cagtttcgtc ttcgatgacc gtatcttcaa
    14521 tgataaactt accttcttca ttcagtgcaa actggaacac ttgatcagat agccgatacc
    14581 catttggtgc gacagattcc gagacctcat attcaccaat agacagatgc agaggttggt
    14641 cgtaatttaa atcgacatac ttttctactc ctacggcacg agaatagaag tatccgatcc
    14701 caggcaaacg tttcgattct tctggttcta acggattacc agtgtatgct cgatacagcg
    14761 ggaaggaaga atcccccact ttcctgatat cgaaacctgc accatctaat gtatggcctt
    14821 catggtctaa ttttctaact cggatctccc gataatcaaa cgtattgtaa gcagtgaatg
    14881 ctacccataa acgatcctct tcatctgttg tatacgtgag atattgccaa ttttcttcac
    14941 ctgtttgctt gagttggata gtccctatag cattatcgta aagtcgcaag atccatgtgt
    15001 cattttcatc aggttctttg aaaccttctg ggttctggct ctcaatgatt ttgtattctg
    15061 catagttggt agtttcttca ctatccttat aaacaaatga atctggctct ccgattggat
    15121 gaataacttt ctcaccatcc acgattacat atttcccgtc ttcatctact gaatagaacg
    15181 tgagtctccc tttctcttcc tctacttcgt tcacaccaga aagccctttc gctactggat
    15241 atccgatttc cagtccgtta ccatctgttt cataaagttc aaactctgcg ccattgattg
    15301 gcatattact gccttcaatt agtttattga tattcatgtg gattggcttg aattcatttc
    15361 gcatttccag tttgacatac aactcatcat actctgtgcc ttctatgtta ggctcttgta
    15421 caacagaaag cttgtgatat tctccttcat aataggaatt cggatcttta ccttccggtc
    15481 cgccgactct cgaaccagat gtatattcat ctacaagctc gtagtgacct aacatctcat
    15541 cttcgttggc tgcatatttt ttgatttgga attgatacgt ctcatctact aactgataac
    15601 gtggtggtcc agcgatctct ctgatccaaa atatgtcgct gactgctcca gtttcatctc
    15661 ctaaaatcgg catcttcgtt tcaaagatcg ctattccgtc cgtggtagtt ttacttgttt
    15721 cgattggttc ggcttcccca ctctctggat tttggtcata taatgcaaat tcgactccag
    15781 ccaacggctc gccattttga cccttcttgc tgacttggaa attacggcct ttatgttcgt
    15841 aacgaatgga agaagatggg aaatacatcc gtccagtagt gatatcattc tcataatcct
    15901 ccaggttctg aatataggcc atattgttca ctgtgcaaaa atttccgtct tgagctcccg
    15961 catcaagttt tacttggtac ttgatcctga ccatttcccc ttcacctaaa gaaatcggag
    16021 aaaatttata agcttggttt tcccgtccga catacgttgt cggagtcgcc actacttctg
    16081 gtggggcttg tccatgacgt tcttcatccc atatttctgg ctcttggtac ttcatgccgt
    16141 tttctcctgg atctagatgg taaaactgaa tatctgccga tctgccagct ccaaatggtg
    16201 agacattaaa accagtttca tcataaagtg tggcttgctg gatcgttctc ttgaaggtag
    16261 aagagatatc ctccaaggct gtaacaatgt cattcttgtt gttcgcattg taatatcgcg
    16321 gtgttacgcc atcttcttct aaatcagcaa tatttttcaa tacatttcgt ccaatagcat
    16381 ctagtcgttg tctcccgcta acgtttccat cgattcctaa accaatggag aagatacttg
    16441 gcgggttctc ttgatacttg tttttcagcc aatgatgata agcaactgtg ttgatcgcag
    16501 atgacttttc ttgcgaagta ggactgccat tcgtattgtt attagaagaa ccatcaccaa
    16561 cccagtgagt cgctttatta tctccttctt ttacctctcc ataacgatag tgtacattta
    16621 cttcatcttc tggacggtta aagtcagatg cataggtaac tggatgagta aaccctgcat
    16681 tagaagtatt cccagaaaac tcctcaaaat ttctgaacaa aacgtcatta tcttcagcaa
    16741 tcttatcttc catcacagtc cagttgctcc aagctccatt agtgctcgta cgataacttg
    16801 actgaatcgg tgcataactg aaggtcggtg tcccatctcc cacaacgatt aggatttttt
    16861 ctggatttct attttctccg ccattgtctt tatacaatgt ttcatagcca tttttaagcc
    16921 ctagtgtaag cggagttcct ccgattggtg tacgatttaa tgctgtattc tcacttcccg
    16981 tcaaacgatt gatatcattt gtcaatggaa agatgtccga actgatttgc tcatggttat
    17041 tgatagctgt actattatgg taaaaattca ccataccgat tcgaatatcc agactcgggt
    17101 cagagagcag atctttactc atttcgttta ctgcctcaat caacgcagca tttttagttt
    17161 gactattggt cccagcggtt aattcactca tactcgctga cttatctaga acaaaaacga
    17221 tatcgattgg actgatttcc tttttctcgc ccccgatgac atctagatag acattgtaaa
    17281 gggttgttgc atcttcagga tgtagatcag atgctgtgct gcttggcaga acaggttcta
    17341 cccatttttt gatgtacgcc ccgttataat ccaagtaact gttttcaaaa ttccgttcat
    17401 ttccccaaag ctgaacggtt tgagctggaa cgtttctatc ttcttcgcct gcagttgatc
    17461 cattattagt agcatagtcg taatttcgcc agttcgctcc cccgtttgta taatcactat
    17521 catcgatcaa gttctctccg tcatatggcg agcctttgtc tccttgatag cgttcattgc
    17581 tttgtgtcca atttcctaac acttttgtat agtcattcgt tgtaaacttc gggtagcgat
    17641 gctgttcgtt ttcaacttct ccgctaggat agatatattc ataggggtct tgcgtagctg
    17701 ttggcccaag acgctccaaa ataccgttga ataactggcg ttgaatgcgc tggagtgctt
    17761 gaccgctggg tacgattggt tcgtccagct tttccatctc attttcttcg ctacgttgaa
    17821 tttcagcgga atcttctgta tcagtttcat tttcttgtaa ttcttggtta tctattcttt
    17881 catcaaccac cacttgaaga ggtagttacc cttcgttcac atcggggatc ttttctgttt
    17941 caaaaatcaa tgtgccggtt tctgcttctt ttgaataaac ttttccgaca taccattctt
    18001 gaccatcgat cgtctcgact tttaggtctt ctccatcttc atcataggag gatagacctg
    18061 ttccacgaac gttttttacg gtaccaatcc cggcggctgc cgcatcgatt gctaaacgta
    18121 ttctaccttc attggcactt tccttttttt caaaagccag atgccattca agggtttctt
    18181 ctttttcaat atacttccct tcaatttttg catagtcctc atctaagaat acctcatcct
    18241 cctttgtttc tgttgactca gcagctacat ccagggtcgt catatgatag actgactgga
    18301 aatatggtcc aaatagcaaa ctgacagtta ctaactgcaa aagcttcaat tttaaccctt
    18361 tcacatcctt atactccttt caaaaatatt tgtcttcttt cacaacaatc aacaaaacaa
    18421 tgcccatcta attataattt aatttaattt taattttaaa tcaaatgata tcctttttag
    18481 ttaaaaaa
//
LOCUS       DAZKLM010000058        17459 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3764
            SAMEA115706913-rid22582563.denovo.059, whole genome shotgun
            sequence.
ACCESSION   DAZKLM010000058 DAZKLM010000000
VERSION     DAZKLM010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706913
            Sequence Read Archive: ERR13196647
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17459)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17459)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287823.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287823.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,004
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,849
            CDSs (with protein)               :: 2,849
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 41 of 96
            Pseudo Genes (incomplete)         :: 52 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 15 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17459
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706913-rid22582563.denovo.059"
                     /isolate="VRE3764"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(107..1405)
                     /gene="eno"
                     /locus_tag="ACG842_001812"
     CDS             complement(107..1405)
                     /gene="eno"
                     /locus_tag="ACG842_001812"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000015 - phosphopyruvate hydratase
                     complex [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HFQ0248348.1"
                     /translation="MSIITDVYAREILDSRGNPTIEVEVYTESGAFGRGMVPSGASTG
                     EYEAVELRDGDKARYGGKGVTKAVDNVNNIIAEAIIGYDVRDQMAIDKAMIALDGTPN
                     KGKLGANAILGVSIAVARAAADYLEVPLYHYLGGFNTKVLPTPMMNIINGGSHADNSI
                     DFQEFMIMPVGAPTFKEALRMGAEVFHALASILKARGLATSVGDEGGFAPNLGSNEEG
                     FEVIIEAIEKAGYVPGKDVVLAMDAASSEFYDKEKGVYVLADSGEGEKTTDEMIKFYE
                     ELVSKYPIISIEDGLDENDWDGFKKLTDVLGDKVQLVGDDLFVTNTQKLSEGIEKGIA
                     NSILIKVNQIGTLTETFEAIEMAKEAGYTAVVSHRSGETEDSTISDIAVATNAGQIKT
                     GSLSRTDRIAKYNQLLRIEDQLGEVAEYKGLKSFYNLKNK"
     gene            complement(1521..2276)
                     /gene="tpiA"
                     /locus_tag="ACG842_001813"
     CDS             complement(1521..2276)
                     /gene="tpiA"
                     /locus_tag="ACG842_001813"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HFQ0248349.1"
                     /translation="MRKPIIAGNWKMNKTASEAKEFAEAVKNKIPSNDKVDSVIGSPA
                     LFLQELMTSAEGTELKISAQNCYWENSGAFTGETSPAALADLGVHYVIIGHSERREYF
                     HETDEDINKKAKAIFANGMTPILCCGESLETYEAGKTAEWIEGQITAGLADLSAEQVS
                     NMVIAYEPIWAIGTGKSADAQIADEICGVVRQTVEKLYGKEVSEAVRIQYGGSVKPEN
                     IAEYMAKENVDGALVGGASLQADSFLSLLDAVK"
     gene            complement(2380..3570)
                     /gene="pgk"
                     /locus_tag="ACG842_001814"
     CDS             complement(2380..3570)
                     /gene="pgk"
                     /locus_tag="ACG842_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005228679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HFQ0248350.1"
                     /translation="MAKKTVKDIDLKDKKVLVRVDFNVPLKDGVITDDTRIKAALPTI
                     NYVLEQGGKAILFSHLGRVKTEEDKEGKSLAPVAKRLGELLGKEVTFVPETRGEQLEE
                     AIRNMNDGDVVVFENTRFEDIDGKKESKNDTELGKYWASLGDVFVNDAFGTAHRAHAS
                     NVGIASTGIPTVAGFLMEKEIKFIGEAVEEPKRPMIAILGGAKVSDKIGVIENLIPKA
                     DKILIGGGMTYTFYEAKGIKIGNSLVEADKVELAKELIEKAGDKLVLPIDNVCAPEFS
                     NDVETQVIEGDIPDGLMALDIGPASVKLFADTLQGAKTVVWNGPMGVFEMSNFAKGTI
                     GVCEAIANLEGATTIIGGGDSAAAAEQLGFADKFTHISTGGGASLELLEGKELPGLAA
                     INDK"
     gene            complement(3680..4681)
                     /gene="gap"
                     /locus_tag="ACG842_001815"
     CDS             complement(3680..4681)
                     /gene="gap"
                     /locus_tag="ACG842_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HFQ0248351.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIQDVDGIEVVAINDLTDAKMLAHL
                     LKYDTTQGRFNGTVEVHEGSFNVNGKEVKVLANRNPEELPWGELGVDIVLECTGFFTS
                     KSAAEKHLTAGAKRVVISAPGGNDVPTIVYNTNHETLTGKETVISGASCTTNCLAPMA
                     KTLNDKFGVVEGLMTTIHAYTGDQMTLDGPHPKGDFRRARAAAANIVPNSTGAAKAIG
                     LVIPELNGKLDGAAQRVPVPTGSLTELVTVLEKEVTVDEINAAMKEASNESYGYNTDE
                     IVSSDIVGMTYGSLFDATQTKVMTVGDKQLVKTVAWYDNEMSYTAQLVRTLEYFANL"
     gene            complement(4722..5813)
                     /locus_tag="ACG842_001816"
     CDS             complement(4722..5813)
                     /locus_tag="ACG842_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="central glycolytic genes regulator"
                     /protein_id="HFQ0248352.1"
                     /translation="MGRFKSTLDVKCPTKEIAMLDEIKMIESVAPDIIEVLQERYKIL
                     RNIYWMQPIGRRSLSESMGITERVLRTETDLLKNLNLIDTSKSGMTLTTKGEEVYQSL
                     ENFMDQLLGTHQTEQQLAEYFGNQRCIVVSGNSEEQTKVADAFGEALSEALDRLLPEG
                     ENIIAVMGGTTMAVVAEQLSNLENKKRHNLFVPARGGIGEAITVQANSVSARMAAKAN
                     GNHRALYVPEQLSLATYNSLLNEPSIQEVLNLISEANCVIHSIGRALHMAARRKMTEK
                     ELVMLKQANAVAESFGYFFNEKGEVVYKVPRIGIELQDLEKVPIIMAIAGGKSKAKAI
                     RAYMKNAPKQTWLITDEAAANEILKGVTL"
     gene            complement(5962..7296)
                     /gene="rpoN"
                     /locus_tag="ACG842_001817"
     CDS             complement(5962..7296)
                     /gene="rpoN"
                     /locus_tag="ACG842_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HFQ0248353.1"
                     /translation="MKFEQNFSQKQQQTQKLAMTQQLQQSIQVLQYNSEELLAFVENQ
                     AMENPLVEVVEPEWQPDYIKASSSSYEGEETNYLNQIPDTKGSLFDSLIEQVHLNYRD
                     TFLRKIVLYLVEYIDLNGFLTISLEEAGKEIGATPIQMLDALTLIQQLEPAGVGARSL
                     QECLMLQTERDDYAPELAYIVLEECFEELVERKWKEIAQRFSVDLHAVQQIFDYLQTL
                     SPSPGRIFDRSSELYIIPDVRVLVDDDKNVQVISNRKNQPNIRFQESYFKQMSQQADK
                     ETENYLKERKQEFEWLKKTILQRGDTILRVAQVIVSRQKAFFIDKERPIKPLTLKEVA
                     TEIDVHESTVSRAVNGKYLETDFGVFELKKFFTTRIPTNSTEQTEDLSADTAKKKLQE
                     LVDQEDKNKPLSDQKLVELLKKDEIAISRRTVAKYRDLLGIPSSSKRKRYDN"
     gene            7356..7427
                     /locus_tag="ACG842_001818"
     tRNA            7356..7427
                     /locus_tag="ACG842_001818"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7389..7391,aa:Arg,seq:ccg)
     gene            complement(7619..8503)
                     /locus_tag="ACG842_001819"
     CDS             complement(7619..8503)
                     /locus_tag="ACG842_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HFQ0248354.1"
                     /translation="MKRNYLVISIIALSVLLFFEITSVASLITEVKGQTHSSEHSSQS
                     FKQSEQSTIKSQSTETANVKEAIQESAEKVQSQSSQSTQDSTKTSRDPAVKSVAISFD
                     DGPGATTTPQLLRILKEKNVHATFFVLGENTAQHPEIVKQTAEAGHEIGNHTYDHQNL
                     ATLSAQSMTEEVTKADTEIKKVTGKTPTFVRPPYGSVTNVGATIIQRPIIEWSVDSED
                     WKTRNPDLILQKIQATVYDGAIILFHDIYPETIRAVPQVIDYLKEQGYRITTVGDLLG
                     HPTAVENYYGRNDHRPVQ"
     gene            complement(8590..9489)
                     /locus_tag="ACG842_001820"
     CDS             complement(8590..9489)
                     /locus_tag="ACG842_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsiV family protein"
                     /protein_id="HFQ0248355.1"
                     /translation="MYSQKENLKKLKKTYKEQAIPKGLKEEIRQRFIIEEQSFIKAKR
                     RKRRLQGFGVSLAIVMITTSSLLFNSQVRSFAEDLPILGSVIELILGERFTDRSEKID
                     IQVPKINTQNEKENKTIHGLNQKYFREGQADFEKAEKEYGNFETDHYQVLGDYQKIVD
                     DNRFLVIERQITQTAADSHVEKRYDTIDKKNSVQLSLPLLFKDDTYLSVLTKEVKRQM
                     AEQVKEDPSKYYWTEQDIQEGTIEKPTLVTPTRSFYLNKEHQLVLTFSQYEIAPGYMG
                     TPEFVIPKSVTKTILASEDYLDH"
     gene            complement(9506..10012)
                     /locus_tag="ACG842_001821"
     CDS             complement(9506..10012)
                     /locus_tag="ACG842_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HFQ0248356.1"
                     /translation="MIFTGYKKKRQIEKYRHLLNGLIETDYQKMFRITMNYVHDKEEA
                     LDVMQDSFHKALNYFEKEKEIEHFSAWFYRILIRTALDSWRRQKRTPVDLFEIQDIPQ
                     QLPLETSVAELHMILDKIDSPEKEILILYFFEGFRLKEIATILDMNENTVKTKMYRSL
                     NALRQILE"
     gene            complement(10201..10821)
                     /gene="rpoE"
                     /locus_tag="ACG842_001822"
     CDS             complement(10201..10821)
                     /gene="rpoE"
                     /locus_tag="ACG842_001822"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HFQ0248357.1"
                     /translation="MEISVFEGANKSELSMIEVAHAILEQRGDVMDFSDLANQIQNYL
                     GKSDSEIRDSLAQFYTDLNIDGSFISLGDNRWGLRSWYAIDSIDEEVNHGMDEEDEDT
                     PRRRKRKKVNAFINDDEDAIDYNDDDPEDTDLTEEDDDDLFDDDDDEDEEIAAYNSDL
                     QEIGADDPTDDEEDLPGNIEEDLSIIDEDDDDEDYEEEEFSDSEKE"
     gene            complement(10897..11334)
                     /locus_tag="ACG842_001823"
     CDS             complement(10897..11334)
                     /locus_tag="ACG842_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HFQ0248358.1"
                     /translation="MELSQGTPISIKLRTKVKQQGEVQDFYFDLKGQMVTIGDTLYIR
                     YKEIQEETGDEIPVTIKLVPDGHVQLIRAGEMRMRLKFGYKERLETSYRTPYGMIQIA
                     TFTKELHVSLKDRPTAGKVRIDYDLFMGPEKIGEYYLTLDFTA"
     gene            11497..12327
                     /locus_tag="ACG842_001824"
     CDS             11497..12327
                     /locus_tag="ACG842_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HFQ0248359.1"
                     /translation="MNPAIRLDQHIYQKNDFSPFALTDILTEYSKEHQQIFLHFWQYD
                     CTVILGMKDVRTPFLSDGIQTLEDAGYAPVIRNSGGLGIVSDEGILNISLIFPQSDTK
                     KITIDDAYEQMLLLTKYAFPKAPIQAFEITHSYCPGTYDLSIHGKKFAGIAQRRIKNG
                     ISVMMYLSVNGNQEKRGQVMKRFYQSALKEDFGTNGYPAVDPATMATLEELLDQPLFV
                     KEVKEAFAKAFEKLYSASDLQDSTDWLKKEIQLEQWNNQIQRMEQRNNIKELHYDNPL
                     "
     gene            12311..13678
                     /locus_tag="ACG842_001825"
     CDS             12311..13678
                     /locus_tag="ACG842_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFQ0248360.1"
                     /translation="MTTPYKYQMLPMEKVFRDPVHNYIHVQHQVILDLINSKEVQRLR
                     RIKQLGTSSFTFHGAEHSRFTHSLGVYEISRRICDIFSRNFSKEKIGNGGWDDQERLV
                     TLCAALLHDVGHGPYSHTFEHIFHTNHEAITVEIITSPETEVYQILNRVEEGFPEKVA
                     SVIQKTYPNPQVVQMISSQIDADRMDYLLRDAYFTGTEYGTFDLTRILRVIRPYEGGI
                     AFSMSGMHAVEDYIVSRYQMYVQVYFHPVSRGMEVILEHLLHRAHELYQEDASSFELH
                     SQLLLPFLDGSFTLEEYLKLDDGVLGTYFTQWSESADPILNDLAKRFLNRKPLKSATF
                     SGNRDSKLVQELTLLVEKVGYNPVYYTAVNSSYDLPYDFYRPEQGRHRTQIEILRNDG
                     TLIELSQVSQLVAALAGQEQGDERFFFPKEMVDPSLRDHYDLFDETYQEFASHIRNGA
                     LIEIN"
     gene            13697..14506
                     /gene="yidA"
                     /locus_tag="ACG842_001826"
     CDS             13697..14506
                     /gene="yidA"
                     /locus_tag="ACG842_001826"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HFQ0248361.1"
                     /translation="MSIKLVAIDIDGTLLNEKREVTKEVKEAIAAAVAKGVSIVLCTG
                     RPLPGVQEQLNELNLFQDNDYVITYNGALVQQTKSGKIIARHGLTHEDFLEIEVMARR
                     VGSHLHSIDDKAIYTPNRDISAYTIHEASLVKMPLKYRTPEEMTPDMNIVKMMMIDEP
                     EILDAAIARLPQTFRDKYTTVKSAPYYFEVLNKEASKGAAVANLAQHLGIDQDEIMAI
                     GDNENDLSMIEYAGLGVAMGNAVSLVKEAANVITSSNDEHGVAEAIKKYVL"
     gene            complement(14549..14992)
                     /locus_tag="ACG842_001827"
     CDS             complement(14549..14992)
                     /locus_tag="ACG842_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HFQ0248362.1"
                     /translation="MDKPIFGEKKQFADYQTRYAAYIVIERDQQIAVIEAPNGAFFLP
                     GGEIEGEETKEEAIVRELIEEMGIVAEISSYLGEADEYFYSNYRATYYYNPGYFFVAD
                     SWKRIGEPTEKTNKIWWVSPQEALVKLKRGSHRWAVERWIEKNKN"
     gene            15126..15497
                     /locus_tag="ACG842_001828"
     CDS             15126..15497
                     /locus_tag="ACG842_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFQ0248363.1"
                     /translation="MKMAHTCVRVKDLEASIEFYQKAFGFEESRRRDFPENKFTLVYL
                     TLPGDDYELELTYNYDHGAYDLGDGYGHIAIAADDLEKLHEQHKAAGLEITDLKGLPG
                     TAPSYYFVIDPDGYKIEVIRG"
     gene            complement(15666..16448)
                     /locus_tag="ACG842_001829"
     CDS             complement(15666..16448)
                     /locus_tag="ACG842_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HFQ0248364.1"
                     /translation="MNLDYYVVDAFADEVFKGNPAAVYVLEEWLPEGTMQKIAIENNL
                     SETAFTVKKNQEFELRWFTPDREIDLCGHATLATAFVLFNYYKIPDETIKFSTQSGNL
                     FVTRIKDYYYMDFPSIMPKKVPILSEYEEAIGAKIKEAYLARDLFFVLEDEKTVAKLQ
                     PDFTAIKNFELGIGVIVTAESVQKDFVSRTFFPKLTINEDPVCGSAHSNLIPYWAERM
                     NKTKLSAYQMSSRGGDLECELKEDRVIIGGKAVLFSKGTSFI"
     gene            complement(16445..17416)
                     /locus_tag="ACG842_001830"
     CDS             complement(16445..17416)
                     /locus_tag="ACG842_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HFQ0248365.1"
                     /translation="MKIINFEEVVASFSFEEAIQVMKKCFFDYESGKISQNPRLVEIL
                     PDGKGKNMFAMMPAYLGENRYFGTKVITAFPDNHQYHLPSHLGEIFLFDAQNGLPKAL
                     INANAVTWIRTAAVSALATDYLANPHAETLALIGAGQQALSHLTAISTIRPITTVFVY
                     DILEERRNTFIAKAKKQYPKITFINAISVEDAVRPAEIICTLTSSKEAFLQKEWVRED
                     AHINAVGTFTPDTREISTSLMETSQIYVDDYSAALTESGDLLIAMAEGDLEESCIVGT
                     LGELVSEKKTFYKGESRPTIFDAVGLAIEDLCCAEYIYNKLEKGALK"
BASE COUNT         4842 a         3626 c         3135 g         5856 t
ORIGIN      
        1 tctctacctg ctgatatttc agcaagtaga gagttcgatt acttaactaa gtgatcatac
       61 tcgactatct ttcaatagtc gatcatgtat cttcattaga aaagaattat ttgtttttta
      121 agttgtagaa agatttcaaa cctttgtatt ctgcaacttc accaagttgg tcttcgatac
      181 gtagtaattg gttgtattta gcaatacggt cagtacgtga tagagaacca gttttgattt
      241 ggccagcatt tgttgcaaca gcgatatcag agattgttga atcttctgtt tcaccagaac
      301 ggtgagatac aactgctgtg tagccagctt ctttagccat ttcgatagct tcgaaagttt
      361 ctgttaatgt accgatttgg ttcactttga taaggattga gttagcaatt cctttttcga
      421 taccttcaga taatttttga gtgtttgtta cgaacaagtc gtcaccaact aattgaactt
      481 tgtcgcctaa tacgtcagtt aatttcttga atccgtccca gtcgttttcg tctaatccat
      541 cttcgattga gatgattgga tatttagaaa ctaattcttc gtagaatttg atcatttcgt
      601 cagttgtttt ttcgccttcg cctgagtcag ctagtacgta aacacctttt tctttgtcgt
      661 agaattctga agaagcagca tccatagcaa gaacaacgtc tttaccaggt acatagccag
      721 ctttttcgat tgcttcgatg attacttcaa aaccttcttc gtttgaacca aggtttggag
      781 caaatccacc ttcgtcacca acagaagttg ctaatccgcg agctttcaag attgaagcta
      841 atgcgtggaa tacttcagca cccatacgta gagcttcttt gaatgttgga gcgccaacag
      901 gcatgatcat gaattcttgg aagtcgattg agttatcagc atgtgatccg ccgttgatga
      961 tgttcatcat tggtgttggc aatacttttg tgttgaatcc gcctaggtag tggtataaag
     1021 gtacttctag gtagtcagca gctgcacgtg caacagcgat agaaacacct aaaatagcgt
     1081 tcgcacctaa tttacctttg ttaggagttc cgtctaaagc gatcatagct ttgtcgatag
     1141 ccatttggtc acgaacatcg tagccaatga ttgcttcagc aatgatgttg tttacgttgt
     1201 cgacagcttt agttacacct ttaccgccat aacgagcttt gtctccgtca cgtaattcaa
     1261 ctgcttcgta ttcaccagtt gaagcacctg aaggaaccat tccgcggcca aaagctccgc
     1321 tttctgtgta tacttctact tcgattgttg ggttaccacg tgagtctaag atctcgcgag
     1381 cgtaaacatc agtaataatt gacatgtttt gtctctcctt tgagtttgtt ttttcctaag
     1441 ggatttccct catatacgat tttagttaat tttgctcatt agtgcaaaca tttcttgcat
     1501 ttttcgcaaa ttttcaacaa ttattttaca gcgtctaaca atgacaaaaa tgaatctgct
     1561 tgcaagcttg cgccaccgac taaagcaccg tcaacatttt cttttgccat gtattcagca
     1621 atattttctg gttttacaga accgccgtat tggatacgaa ctgcttctga tacttctttt
     1681 ccataaagtt tttcaactgt ttgacgtaca actccacaaa tttcgtcagc gatttgtgcg
     1741 tcagctgatt taccagtacc gattgcccag attggttcat aagcaataac catgtttgac
     1801 acttgttcag ctgaaagatc agctaaacca gctgtaattt gtccttcgat ccattcagca
     1861 gttttgcctg cttcataggt ttctaaagat tcgccgcagc ataggattgg tgtcatacca
     1921 tttgcaaaga tagcttttgc ttttttattg atgtcttcgt ctgtttcatg gaaatattca
     1981 cgacgttctg aatgtccgat gatcacataa tgaacaccta gatcagcaag agctgctggt
     2041 gaagtttcac cagtgaacgc acctgagttt tcccagtagc agttttgtgc tgagattttt
     2101 agttctgtac cttcagctga tgtcattaat tcttgtaaga ataatgcagg agatccgatg
     2161 actgaatcaa ctttgtcatt tgaagggatt ttgtttttta ctgcttcagc aaattctttt
     2221 gcttctgatg cagttttgtt cattttccag ttacctgcga taataggttt acgcattgag
     2281 gaacacactc cttaatttat tgaaaaatgg ctagggagtc atgaaaactc ccttcacctt
     2341 ttgatcgatt actcgtcaga attgagcaaa cttggtcgtt tatttatcgt tgattgctgc
     2401 tagtcctggt aattctttac cttcaagcaa ttccaagctt gcgccaccac ctgttgagat
     2461 gtgtgtgaat ttgtcagcaa atcctaattg ttctgctgct gcagctgagt ctccgccacc
     2521 gatgattgtt gttgcgcctt ctaagttagc gattgcttca caaacaccga ttgtaccttt
     2581 agcaaagtta ctcatttcaa atacacccat aggtccgttc caaacaactg tttttgcacc
     2641 ttgtaatgta tcagcaaata attttactga tgctgggccg atgtctaaag ccatcaaacc
     2701 atcaggaatg tctccttcga ttacttgtgt ttcaacatca tttgaaaatt ctggtgcaca
     2761 gacattgtcg attggcaaga caagtttgtc gcctgctttt tcgattaatt cttttgctag
     2821 ttccacttta tcagcttcta ctagagagtt accgatcttg attccttttg cttcgtagaa
     2881 tgtgtaagtc attccgccac cgatcaagat tttatctgct ttaggaatca agttttcgat
     2941 tacgccgatt ttatcagaaa cttttgcgcc accaaggatt gcaatcattg gtcgttttgg
     3001 ttcttctact gcttcaccga tgaacttgat ttctttttcc atcaagaatc cagcaactgt
     3061 tgggatacca gttgaagcga tacctacgtt agaagcatgt gcacggtgag ctgtaccgaa
     3121 agcatcgtta acaaatacat cgcctaatga agcccagtat ttacctagtt ctgtatcatt
     3181 tttgctttct tttttaccgt caatatcttc aaatcgtgta ttttcgaata cgacaacgtc
     3241 gccgtcattc atgttgcgga ttgcttcttc taattgttct ccgcgagttt caggtacgaa
     3301 tgttacttct ttacctaata attcgcctag acgttttgca actggagcta aagattttcc
     3361 ttctttatct tcttctgttt ttacacgtcc aaggtgagag aaaaggattg cttttccgcc
     3421 ttgttctaga acgtagttga ttgttggcaa tgctgctttg atacgagtgt cgtcagtgat
     3481 cacgccgtct ttcaaaggaa cgttgaagtc aacacggaca agaacttttt tgtcttttaa
     3541 atcgatatct ttcactgttt tcttagccat tttatttcct cctgaataaa cttattatca
     3601 aaaaaagcgg aagaagcgcg attgcttccc cgcttttaaa tgtacatgtc tataatttac
     3661 acttgtacgt ttgtctatct tataagttag cgaagtattc taaagtacgt actaattgtg
     3721 cagtgtatga catttcgttg tcgtaccaag caacagtttt aactaattgt ttgtcgccaa
     3781 ctgtcattac tttagtttgt gtagcatcaa ataatgaacc gtaagtcata ccaacgatat
     3841 ctgaagaaac gatttcgtct gtgttgtatc cgtaagattc gtttgaagct tctttcattg
     3901 ctgcattgat ttcgtcaaca gttacttctt tttcaagaac tgttactaat tctgttaatg
     3961 aacctgttgg tacaggaaca cgttgagcag ctccgtctaa tttaccgttc aattcaggaa
     4021 ttactaaacc gatagcttta gcagcacctg ttgagttagg aacgatgttt gcagcagcag
     4081 cgcgtgcacg gcggaagtca cctttaggat gaggtccgtc tagagtcatt tggtcacctg
     4141 tgtaagcgtg gatagttgtc attaatcctt caacaacacc aaatttgtcg ttcaatgttt
     4201 tagccatagg agctaaacag ttagtagtac atgaagcacc tgagataact gtttctttac
     4261 cagttaatgt ttcgtggttt gtgttataaa cgattgttgg aacatcgttt ccgccaggag
     4321 cagagataac aacgcgttta gcaccagctg ttaaatgttt ttcagctgct gatttagaag
     4381 tgaagaaacc agtacattct agaacgatat ctacgcctag ttcgccccat ggtaattctt
     4441 ctgggttgcg gttagctaat actttaactt cttttccgtt tacgttgaat gaaccttcat
     4501 gaacttcaac tgttccgttg aaacgtcctt gagttgtgtc gtattttaac aagtgtgcca
     4561 acattttagc atctgtcaag tcgttgattg ctactacttc gattccatct acatcttgga
     4621 tacgacggaa tgctagacgt ccaatacgtc caaaaccatt aatacctact ttaactgtca
     4681 tttaagattt cctccttatg aaaatcaaaa aaatatttat tttaaagggt tacccctttt
     4741 aaaatctcat ttgcagcagc ttcatcagtg atgagccacg tttgttttgg tgcatttttc
     4801 atataagctc gaattgcttt agcttttgat tttccgcccg cgattgccat gatgatcggg
     4861 actttttcca aatcttgcaa ttctatgccg attctaggga ccttataaac gacttcacct
     4921 ttttcattga aaaagtagcc aaaagattcc gcaacagcat ttgcttgttt cagcatgact
     4981 aattcttttt cagtcatctt acgacgtgct gccatgtgta atgcacgccc aatactatga
     5041 ataacacaat tcgcttcact aatcaagttt aagacttctt ggatagatgg ctcattgagt
     5101 aaagaattgt aagttgccag acttaattgt tccggaacat aaagtgcacg gtggttaccg
     5161 tttgctttag cagccattct tgcactgaca gaatttgcct gtacagttat cgcttcacca
     5221 atcccgcctc ttgcagggac aaacaggtta tgccgtttct tattttccag attagacaat
     5281 tgttcggcta ccacagccat agtagttcct cccatcacag cgatgatatt ctcgccttct
     5341 ggaagtagtc ggtctagggc ttcgctcagc gcttcgccga atgcatcagc cactttggtc
     5401 tgctcttcgc tgtttcctga aacaacgata cagcgttggt taccaaaata ttctgccaac
     5461 tgttgttcgg tttgatgcgt acctaaaagc tgatccatga agttttcaag gctttgataa
     5521 acttcttcgc ccttagttgt cagcgtcata ccgctttttg aggtgtcgat caaattcaga
     5581 tttttcaaaa gatctgtttc tgttcgcaat actcgttctg tgatccccat actttccgat
     5641 aaacttcgac gtccaatcgg ctgcatccaa tagatatttc gtaaaatttt atagcgttct
     5701 tgcaacactt cgataatatc tggtgcaaca gattcaatca tttttatttc atccagcata
     5761 gcgatctcct tagttggaca ctttacgtcc aatgtagact taaaacgacc catatacgat
     5821 aaaaaaagaa attaactatt cgaatttcac atcaataaag ggaaatctca acctcacgac
     5881 tgtaagtcta acagttttgc tctcgttttt caactaaaaa gactagaaaa agtgaaatac
     5941 tttttctagt cttttgactg attagttatc ataacgtttt ctttttgatg aactcgggat
     6001 tcctaataaa tcacgatatt ttgctactgt tcgtcttgag atcgctatct catctttttt
     6061 cagcaattca accaatttct ggtcagacaa cggcttgttt ttgtcttctt gatcgactaa
     6121 ttcctgcagc tttttcttcg ctgtatctgc tgaaaggtct tctgtctgtt ctgtactatt
     6181 cgtaggaatt cgtgtagtga aaaatttttt taattcaaat acaccgaagt ctgtttccaa
     6241 atatttcccg ttgactgcac gactgaccgt ggattcatgc acatctatct cagttgcaac
     6301 ttcctttaac gtaagaggct tgattggacg ttccttgtcg ataaagaaag ctttttgccg
     6361 ggaaacaatg acttgagcaa cccgcaaaat tgtatcccca cgttgtagga tcgttttctt
     6421 caaccattcg aattcctgtt tccgttcttt taaataattt tcagtttctt tatctgcttg
     6481 ttgtgacatt tgtttgaagt aactctcttg aaagcgtata tttggctgat tttttcggtt
     6541 tgagataact tgtacatttt tatcgtcgtc cactaatacc cggacatcag gaataatgta
     6601 caattcgctg ctacggtcga aaatccggcc agggcttgga ctcaacgttt gtaaatagtc
     6661 gaatatctgc tgaacagcat gtagatcaac agaaaaacgt tgtgcgattt ctttccattt
     6721 tcgctccacc agttcctcaa aacattcttc caacacgata taagctaatt ccggcgcata
     6781 atcatctcgt tctgtttgaa gcatgagaca ttcttgcaaa ctacgtgcac ctactccagc
     6841 tggttctaat tgctgaatca gagtcaacgc atcgagcatt tggataggcg tagctccaat
     6901 ctcttttccg gcttcttcta aactaatcgt caaaaatccg ttcaaatcga tgtattctac
     6961 aagataaaga acgattttcc tcaaaaaagt atctcggtaa ttcaaatgga cttgttcgat
     7021 caatgaatca aataaagacc cttttgtatc aggaatctga tttaggtaat ttgtctcttc
     7081 tccttcataa gaagatgaag aagcttttat gtaatctggc tgccattccg gctcaacaac
     7141 ttccaccaat ggattttcca ttgcttgatt ctcaacgaat gccaacagct cctcactgtt
     7201 atattgtaaa acttggatgg actgttgcaa ctgttgcgtc atggctagtt tctgtgtctg
     7261 ctgctgtttt tgtgaaaaat tttgctcaaa tttcattttt ttgtccaatc cccttgtaat
     7321 tttttatctg tttagttaca attatcccag tgcttgcgtc cttagtgtag tggatatcac
     7381 gtaagattcc ggttcttgag acgggggttc gattccctca ggacgcgttc tactgctatt
     7441 ttagcataat ttacgaatag ttgcttcttt atttttaagt gaaaactgtc cgccaaagaa
     7501 aattggtgga cagttttttg ttgtacctgt atagatattg ttaagccttt gcaagtaagg
     7561 tagttatgtg ttcaactact tattaaaatg tgttatttat taaagatact tgtctcactt
     7621 actgaactgg ccgatgatcg tttctgccat aatagttctc gacagccgtt ggatgcccta
     7681 ataaatcccc aactgtcgtg atgcgatacc cttgctcttt cagataatcg atcacttgag
     7741 gaacggcacg aatcgtttca ggatagatat catggaataa aataattgcc ccatcgtata
     7801 ctgttgcttg gatcttctgc aaaatcaaat caggatttct tgtcttccaa tcttctgaat
     7861 caacagacca ttcgatgatc ggtcgctgga tgatcgttgc tcctacattc gtgacagaac
     7921 catagggcgg acgaacaaaa gtgggggttt tgccagtgac ttttttgatc tcagtgtctg
     7981 ctttcgtcac ttcctccgtc atcgactggg cagaaagagt ggccagattc tgatgatcgt
     8041 atgtgtgatt gccaatctcg tgccctgctt cagctgtctg cttcacgatt tcaggatgct
     8101 gcgccgtatt ttctcctaag acgaaaaaag tggcatgcac gtttttttct tttaagattc
     8161 gcagaagctg cggtgttgtg gtcgctcctg gtccatcatc aaagctgatt gcgacgcttt
     8221 tgacagccgg atctcggctc gtttttgtac tgtcttgtgt tgattgtgat gactgacttt
     8281 gtaccttttc cgctgattct tgaatggctt ctttgacgtt agctgtttca gttgattggc
     8341 ttttaatggt tgattgttct gattgtttga aagattgtga cgaatgctcc gaagagtgtg
     8401 tttgtccttt tacttcggtg atcaaggagg ccaccgaagt aatttcgaaa aacaaaagca
     8461 cggataaagc aataatactg ataactaaat aatttctttt cactatatct cttctttctc
     8521 tttttctctt cttattattt acttgtactt acttgaaaat tgaaactgct agtcgcaact
     8581 acttctcgtc taatgatcca aataatcttc cgatgccaaa atcgtttttg tcacggactt
     8641 aggaatgaca aattctggtg tacccatata accaggagca atttcgtatt gagaaaaagt
     8701 caaaactaat tgatgctctt tgttcaaata aaaacttcgt gtaggcgtaa caagtgttgg
     8761 tttttctatc gtcccttctt gaatatcttg ctcagtccaa tagtacttag atggatcttc
     8821 cttgacttgt tcggccatct gccgtttgac ttcttttgtc aaaacagata gataggtgtc
     8881 atctttgaat aaaagtggaa gtgagagctg gacactgttt ttcttgtcga ttgtgtcgta
     8941 tcttttttca acatggctat ctgctgctgt ttgggtgatc tgcctttcaa tcacgagaaa
     9001 ccggttatcg tctacgattt tctgataatc tcccaaaact tggtaatgat ccgtttcaaa
     9061 atttccatat tctttttcag ctttttcaaa atctgcttgt ccttcgcgaa agtatttttg
     9121 atttaatcca tgaattgttt tattttcttt ctcattttgt gtattgatct tcggtacctg
     9181 aatatcgatt ttttcagatc gatcagtaaa gcgttcgcct aagatcaatt caatgactga
     9241 tcctaagatg ggtaaatctt ctgcaaagct tcgaacttga ctattaaaaa gaaggctact
     9301 tgttgtaatc attacgatag ccaaagatac tccgaaccct tggagacgtc tctttctccg
     9361 ctttgctttt ataaatgatt gctcttcgat aataaaacgc tggcggatct cctctttaag
     9421 ccctttggga attgcttgtt ctttataagt tttctttagt ttttttaaat tttctttttg
     9481 ggaatacatg gcactcttct ctccttcatt ctaggatttg acgtaaagca ttcaatgagc
     9541 ggtacatttt cgttttaact gtgttttcat tcatatccaa gatagtcgca atttctttca
     9601 aacgaaatcc ttcaaaaaag tagaggatca gaatctcttt ttccggactg tctattttat
     9661 ctaaaatcat atggagctct gcaacggaag tttctaaagg taactgttgg gggatatctt
     9721 gaatttcaaa taagtccaca ggcgtccttt tttgtctgcg ccaactatct aatgccgtgc
     9781 ggatcaatat ccgataaaac catgcactaa aatgctcgat ctctttttct ttttcaaaat
     9841 agttcagggc tttatgaaaa ctatcttgca tcacatctaa ggcctcttct ttatcatgta
     9901 cgtaattcat cgtgatacgg aacatttttt gataatcagt ttcgatgagt ccattcagca
     9961 ggtgacggta tttttcgatc tgccgttttt tcttatatcc agtaaaaatc attctccact
    10021 cctttctgcc tcacttaata tgacgtacaa ggtgcgctaa aagtttcaat ttttttattt
    10081 tttatgtatt tacttgggat ttttatatac aaaaaagaag gtgtgacaaa aatcgtgtca
    10141 cacctcctta ttctgatagg ctgatcgtct ctgtcacaac ctctcgtaag aaaataatgt
    10201 ttattctttt tcagagtcag aaaattcttc ctcttcataa tcttcatcat catcgtcttc
    10261 gtctatgata cttaaatctt cttcgatatt tcctggcaag tcttcttcat catctgtcgg
    10321 gtcatctgct ccgatttctt gcaagtcaga attataagca gcgatttctt catcttcatc
    10381 atcatcgtca tcaaacagat catcgtcatc ttcttctgtc agatctgtat cttctgggtc
    10441 atcgtcattg taatcgatcg cgtcttcatc atcattgata aaggcgttga ctttcttgcg
    10501 tttacggcga cgtggagtat cttcatcctc ttcgtccatt ccatggttta cttcttcatc
    10561 gatcgaatcg atagcatacc atgaacgcaa gccccaacga ttatccccta atgagataaa
    10621 gctgccgtca atattcaagt cagtataaaa ttgtgcgagt gaatcacgaa tttcactgtc
    10681 tgatttccca agatagtttt ggatctggtt agctaaatca gaaaaatcca tgacatcgcc
    10741 acgttgttct aagatcgcgt gtgctacttc gatcattgat aattcgcttt tgtttgcacc
    10801 ttcaaataca ctaatttcca atcaggacac gtcctttcac agtctactcc ttatcataca
    10861 aaatcagaca caaaaaatca aacttttctt ttatatttac gctgtaaaat ccaaggtcaa
    10921 ataatattcg ccgatttttt ccggtcccat gaacagatca taatcgattc gaaccttccc
    10981 tgctgtggga cggtctttca aactaacatg cagttctttc gtaaaagtag caatctggat
    11041 cataccgtat ggtgttcgat aggatgtttc taaccgttct ttatagccaa atttcaaacg
    11101 catccgcatt tctcctgcac ggatcagctg tacatgacca tctggaacta atttgatcgt
    11161 tacaggaatc tcatcgcccg tctcctcctg aatctcttta taacgtatgt acagcgtatc
    11221 gccaattgtc accatttgcc ctttcaaatc aaaataaaaa tcttgcactt ccccttgctg
    11281 ttttactttt gtacgcaatt tgattgaaat cggtgttcct tgtgataatt ccattattca
    11341 ttctccttta cttctcatag ctacattgtt cctagtttct tttgttttgt caaccaactt
    11401 gtccattttt acagaaaacc ttttttccaa aacaactctt tgattttaga gtataatatt
    11461 gtagaaaata tgaatttata caaaggagcc ggttgaatga accctgcgat tcgactagat
    11521 cagcacattt atcaaaaaaa cgacttttcc ccttttgcat taacagatat attaacggaa
    11581 tattctaaag aacaccagca aatcttttta catttctggc aatatgactg cacagtcatc
    11641 ttaggtatga aggatgttcg aactcctttt ctaagtgacg ggatccaaac actggaagat
    11701 gcaggttacg ctccagtgat cagaaattca ggtggattag ggatcgtatc agatgaagga
    11761 attttgaata tttctttgat tttcccccaa agtgacacaa aaaaaataac catagatgac
    11821 gcttacgagc aaatgctcct attgactaaa tacgcttttc caaaggcacc gatacaagca
    11881 ttcgaaatca cccattctta ttgtcctgga acttatgatt taagcatcca tggcaaaaaa
    11941 tttgccggta tcgctcagcg tcggatcaaa aatgggattt ctgttatgat gtacttaagt
    12001 gtcaacggta atcaggaaaa acgtgggcaa gtcatgaagc gattttatca atctgcttta
    12061 aaagaagact ttggtacaaa tggctatcct gcagtggatc ctgctacaat ggcaacactt
    12121 gaagaactgc ttgaccaacc cctttttgta aaagaagtca aagaagcatt tgcaaaagct
    12181 tttgaaaagc tgtattctgc aagtgattta caggattcta ccgactggct aaaaaaagaa
    12241 atccagctgg agcaatggaa caaccaaatc cagagaatgg aacaacgtaa taacataaag
    12301 gagctacatt atgacaaccc cttataaata tcaaatgctg cctatggaaa aagtttttcg
    12361 tgatccagtc cataactata tccatgtcca gcatcaagta atcttagatt tgatcaattc
    12421 gaaagaagtc caacgtctac gacgcattaa acaattaggc acttcttctt tcacttttca
    12481 cggagcagaa catagccgtt ttactcattc attaggcgtt tatgagattt caagacgaat
    12541 ctgcgatatc ttttctcgaa atttttcaaa agaaaaaata ggcaacggag gctgggatga
    12601 tcaggaacgg ctggtgactt tatgtgcggc actgttgcat gatgtcggtc atggtcctta
    12661 ctctcataca tttgaacata tcttccatac gaatcatgaa gccatcactg tagagatcat
    12721 cacttctcca gaaacagaag tgtatcagat tcttaaccga gtagaagaag gttttcctga
    12781 aaaggtagct agtgtgatcc aaaaaactta ccccaaccca caagttgtcc aaatgatttc
    12841 tagtcagatc gatgcagatc gaatggacta tttgcttcgg gatgcttatt tcactggaac
    12901 agaatatgga acatttgatc ttacccgaat attacgtgtg attcgtcctt atgaaggcgg
    12961 gatcgctttt tccatgagtg gcatgcatgc agtggaagat tatatcgtta gccgatatca
    13021 aatgtatgtg caagtgtact tccatcctgt ttcccgtggt atggaagtaa ttttggagca
    13081 tctgcttcat cgcgcacacg agttgtatca agaagatgcc agcagttttg aactccattc
    13141 tcaattactt ctgccatttc ttgacggaag ctttacttta gaagaatacc tcaaacttga
    13201 tgatggggtc ttgggcactt actttactca atggtctgaa tctgctgatc ctattttaaa
    13261 tgatttagct aaacgttttt tgaatcgtaa accactgaaa tctgctactt tttctggcaa
    13321 ccgggattca aaattagtcc aagaactcac tttgttagta gaaaaggtcg gatataatcc
    13381 tgtttattac actgctgtga attctagcta cgatctacct tatgattttt atcgcccaga
    13441 acaaggcaga catcgcacac aaatcgagat tttgcgaaat gacggaactt tgatagaatt
    13501 atcacaggtt agtcagcttg ttgctgcatt agcgggacaa gaacaaggag atgagcgatt
    13561 tttctttcca aaagaaatgg ttgatccgtc cttgcgtgat cattatgatt tatttgacga
    13621 aacttatcaa gaatttgcca gccatatccg taatggcgca ttgattgaaa taaattaaaa
    13681 tatattggag gaaattatgt ctatcaaact agtcgctatc gacatcgatg gtactttatt
    13741 aaatgagaaa agagaagtaa caaaagaagt aaaagaagcg attgccgctg ctgttgcaaa
    13801 aggtgtttcg atcgttttat gcactggccg tcccttacca ggtgtccagg aacagttaaa
    13861 cgagttgaac ctttttcaag ataatgatta cgttattact tataacgggg cactcgtaca
    13921 acaaacgaaa tcaggtaaaa tcatcgcccg tcacggactg acacatgaag atttcctaga
    13981 gatcgaagta atggcacgcc gtgtcggcag tcatctccat tcgatcgatg acaaagcgat
    14041 ctacacgcct aatcgagata tcagcgctta tacgatccac gaagcaagtt tagtcaagat
    14101 gccgctaaaa tatcgaacac ctgaagaaat gacaccagac atgaacatcg tcaaaatgat
    14161 gatgatcgac gaaccagaaa ttttagatgc agcaattgcg cgtttaccgc aaactttccg
    14221 tgataaatac acaacggtca aaagtgcgcc ctattacttc gaagtactaa ataaagaagc
    14281 aagtaaaggt gcggcagttg cgaatctcgc acagcacctt ggtattgatc aagatgaaat
    14341 catggctatc ggagataacg agaacgactt atccatgatt gaatatgctg gtctaggagt
    14401 agcaatggga aatgccgttt ctcttgtcaa agaagcagca aatgtcatca cttcatcaaa
    14461 cgacgagcac ggtgtagctg aagcaatcaa aaaatatgta ctgtaataaa taaaaaaact
    14521 gagagaaagc tgatctactc gctttctttc agtttttatt tttttcgatc catctctcga
    14581 ctgcccatcg gtggctacca cgtttcaatt tcacaagcgc ttcttgaggg ctcacccacc
    14641 aaattttatt ggtcttttca gttggttctc ctatacgctt ccaagaatca gcaacaaaga
    14701 aatatcctgg attataataa taagttgcac gataattcga gtaaaaatac tcatcagctt
    14761 ctcccagata agaagaaatt tctgccacaa ttcccatttc ttctatcagc tcacggacaa
    14821 tcgcttcttc tttcgtctct tctccttcaa tttccccgcc aggaagaaaa aaagcaccat
    14881 ttggagcctc gattactgca atctgctgat ctctttctat gacaatatat gctgcataac
    14941 gggtttgata atcagcaaat tgcttttttt caccaaaaat tggtttatcc atgaatagtt
    15001 tccaccttaa cagttttatt ttctatccat tttacatgag aaatttctca tgaacaagca
    15061 caaatcaact atttccttac ttctttttgt tatgattgag taaaaggaaa aggagagatg
    15121 aatctatgaa aatggctcac acatgtgttc gagtgaaaga tcttgaagca tcaatcgaat
    15181 tttatcaaaa ggcatttggt tttgaagaaa gccgccgccg tgattttcca gaaaacaaat
    15241 ttacacttgt ttatttgaca cttcctgggg atgactatga attagagttg acttacaact
    15301 atgatcatgg tgcgtatgat ctaggagacg gctatggtca tatcgctata gcagctgatg
    15361 atctagaaaa attacacgag caacataaag ctgctggtct tgagattact gacttaaaag
    15421 gattacctgg cactgcgcct tcttactact ttgtcattga ccctgatggt tataaaatcg
    15481 aagtcatccg aggatagtta gtatggggca ttttactctt gattgaataa aaaacaaaca
    15541 ccaataatta aaatgagacg caaacttttc attaaattga aaaagtttgc gtcttttttt
    15601 aacaaccatt gtagctataa ggttgccact ctccaccaat ttatttttaa ttcatttgaa
    15661 agaaatcaaa taaacgaggt acctttagaa aacaaaactg cttttcctcc tattatcaca
    15721 cgatcttctt ttaattcaca ttctagatcc ccgccccttg atgacatttg ataagctgac
    15781 aattttgttt tattcattct ttctgcccaa taaggaataa gattagaatg tgctgatcca
    15841 catactggat cttcattgat tgttaatttt ggaaagaagg ttctagaaac aaagtctttt
    15901 tgcacacttt ctgctgtcac gatgactcct attcctaatt caaaattttt gatagctgta
    15961 aaatctggct gcaatttagc caccgttttt tcatcttcta atacgaaaaa taaatcacgt
    16021 gctaagtagg cttctttaat cttagcccca atcgcttctt catattcaga caatatcggc
    16081 acttttttcg gcataatact aggaaaatcc atgtaataat aatctttaat tcttgtcaca
    16141 aatagattac cgctttgagt agaaaattta atcgtttcat caggtatttt gtaatagtta
    16201 aataaaacaa atgctgttgc taatgtcgcg tgaccacaaa gatcgatttc tcgatctggc
    16261 gtaaaccatc taagctcaaa ttcttggttt ttcttcactg taaatgctgt ttcagataaa
    16321 ttgttttcaa tggcaatttt ttgcatcgta ccttctggca gccattcttc tagcacataa
    16381 accgctgctg gatttccttt gaacacctca tctgcaaatg catcaactac gtaataatct
    16441 aagttcattt taaagcccct ttctctaatt tgttataaat atattctgca caacaaagat
    16501 cttcaatagc tagaccaact gcgtcgaaaa tcgtcggcct cgattctcct ttataaaatg
    16561 tttttttctc agaaactaat tcgcctaatg taccaacaat acaagattcc tctaaatctc
    16621 cttctgccat ggctataagc aggtccccgc tttctgttaa ggcagctgag taatcatcta
    16681 catagatttg agaagtttcc atcaacgatg tacttatctc tcttgtatca ggagtaaatg
    16741 tgcccacagc attgatatgt gcgtcttccc ttacccactc tttttgaaga aatgcctctt
    16801 tactagaggt caacgtacaa atgatttccg ctggacgtac tgcatcttct actgatattg
    16861 catttataaa cgttatttta ggatactgtt ttttagcttt agcgataaaa gtatttcgtc
    16921 tttcttctaa aatatcgtaa acaaacactg tagtgatggg acgaatggta gaaatcgcag
    16981 tcaaatgcga caatgcttgt tgaccagctc caattaaagc aagtgtttct gcatgcggat
    17041 ttgctaagta atctgttgcc agcgcagata cagcagctgt tcgaatccag gtgactgcat
    17101 ttgcgtttat caatgctttg ggtagtccgt tttgagcatc aaacaagaaa atctctccta
    17161 aatgagaagg taaatgatac tgatgattat ccggaaaagc ggtaatgact tttgtgccaa
    17221 aatatctgtt ctcccccaaa taagcaggca tcatcgcaaa catatttttg ccttttccgt
    17281 caggtaaaat ttccaccaat cgaggatttt ggctgatttt accactttca taatcaaaaa
    17341 aacatttttt cattacttga atcgcttctt caaaactaaa agaagctact acttcctcaa
    17401 aattaataat tttcatagac aattccctcc tcacaaaaga ttaacttcta aaatcaata
//
LOCUS       DAZKLM010000059        16220 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3764
            SAMEA115706913-rid22582563.denovo.061, whole genome shotgun
            sequence.
ACCESSION   DAZKLM010000059 DAZKLM010000000
VERSION     DAZKLM010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706913
            Sequence Read Archive: ERR13196647
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16220)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16220)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287823.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287823.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,004
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,849
            CDSs (with protein)               :: 2,849
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 41 of 96
            Pseudo Genes (incomplete)         :: 52 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 15 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16220
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706913-rid22582563.denovo.061"
                     /isolate="VRE3764"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..477
                     /gene="scm"
                     /locus_tag="ACG842_001831"
     CDS             <1..477
                     /gene="scm"
                     /locus_tag="ACG842_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HFQ0248366.1"
                     /translation="STDTSASSDSTDTSTTTSDSTDTSASSDSTDTTSTTSDSSDSST
                     TTSDSTDTSASSDSTDTSSTTSDSSDSSTSSDSTDTSSTTSDTRHASVSFSKTSSDNR
                     TNFNLTNKTSNDNGNKSKRALPKTGSQSNNWITLAGVILLVIGLRITFMSYKKSKR"
     gene            complement(632..1618)
                     /locus_tag="ACG842_001832"
     CDS             complement(632..1618)
                     /locus_tag="ACG842_001832"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HFQ0248367.1"
                     /translation="MKLHPMWENYPALSKELTETLKRMEETVRLKNKPVEQAIKETIH
                     AGGKLLRPAYQLLFSQFGPEQDRDKAIALAASIEMLHTATLIHDDIVDEADLRRGQPN
                     IRSQFGNVVSVYAGDYLFVCCFKLLSDYSTSLKSLQLNSRSMEKVLNGELGQMDDRYN
                     YELSVKEYLENISGKTAELFQLSCSVGAFESGTSERFAKKAGDIGLSIGMAFQIIDDI
                     LDYTKESQEIGKPVLEDMRQGVYSLPLIYSLQKNKPKLLPYLEKKAAMTEEDVDAVRK
                     IVEHTGGVEEARKLAASYTEKALKEIKKLPATSLRTKENLFSLTQLILDRKD"
     gene            complement(1641..2207)
                     /locus_tag="ACG842_001833"
     CDS             complement(1641..2207)
                     /locus_tag="ACG842_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HFQ0248368.1"
                     /translation="MSKLQKMIYISLLVAQGVIIGLLENMIPYPFSFAPGAKLGLANL
                     ITIVAIFTMKKRDSFLLLWLRLFLTTLLGGTISTFLYSMSGALLSYFGMLLVKQLGPK
                     RVSIIGISAAGGFMHNVGQLLMASFIAQSWTVMLYLPVLSVLGILSGIAIGIAANYLL
                     RHVQTLQRFQKDYEQGTKQKWSPMFTKK"
     gene            complement(2221..2637)
                     /locus_tag="ACG842_001834"
     CDS             complement(2221..2637)
                     /locus_tag="ACG842_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HFQ0248369.1"
                     /translation="MSLKEFAEKSLLKPWDVFIILLLTVLSFLPVMIFGLQQNTATAE
                     KEAVLRVDGTEIKTFPLMAGEESNTYTYKDEHGDYNLIEIDGDRVRIKEADCDDQICV
                     RRGWATKNGETIVCLPHKLVIEVRSTDGGDEDSLIY"
     gene            complement(3094..5028)
                     /locus_tag="ACG842_001835"
     CDS             complement(3094..5028)
                     /locus_tag="ACG842_001835"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFQ0248370.1"
                     /translation="MAKQNIVVVGAGYAGVSATKYLAKKLKKEDVAITLIDRHSYHTM
                     MTELHEVAGGRVEPEAIQYDLQHLFARQKNVQLVTDTVIGIDKERKIVKTKLGSYPFD
                     QLILGMGGEPNDFGTPGVKAHGFTLWSFEDSVRIREHIEKTVAKAALEPDDELRKAML
                     TFVVCGSGFTGIEMIGELIDWKNRLAKDYKLDPNEITLMVVEAMPTILNMLDRNDAAK
                     AERYLRKHNVELLLNAPIVEVAADHIKLKDGRNIPTHTLIWTAGVKGTSDAADFGLES
                     ARGQRLIANEYMQAKGYEDKNIYIIGDLVFYEEFPGTPTPQIVQAAEQTGHTAAANIV
                     ASIKGGEKHKFKGNYQGFMVSIGAKWGVANLFDKIHLSGFLAIVMKHIVNLKYFFDIR
                     SGYYMFQYLMHEFFHIKDDRNVTRGHSSRYGNVLWSVPLRIFYGLVWFVEASKKIIGN
                     GEVFKPSTWFGEGSWFTGNSVFPWTWEYNVTDVASGASAAGDATTAASGAGASAGEAA
                     TQAAHFGLSYAYGEEPMAVIEKSPDWFTKIMQVIMPNNDVAMFFQKFMVFFEIALALA
                     LIAGLFTWLCSATTIVLVITFCLSGMFYWVNIWFIFVAFALMNGSGRAIGLDRWVIPW
                     LQRTLGNWWYGRPSSQYGKK"
     gene            5330..6265
                     /gene="pplA"
                     /locus_tag="ACG842_001836"
     CDS             5330..6265
                     /gene="pplA"
                     /locus_tag="ACG842_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular electron transfer flavoprotein
                     PplA"
                     /protein_id="HFQ0248371.1"
                     /translation="MKKKRFVTGFAVLAFSALVLGACGADDNGSSNSSSESSTAQSST
                     AKSTTESSAKVVAGGDLQDGTYKLEEKNYSNGYRAVFEMVVKDGKITESKYDNVNENG
                     ESKTKDAEYNKNMEAKSGTNPEKYIPELNDQFLKAQSASGVEVVTGATHSSESFQNYA
                     QQLIQAAQAGNTDTIEIDNGADLKDGTYKLEEKNYSNGYRVQFEMTVAGGKVTESNFD
                     YIDKDGKSKQDDTEYNENMKAKSGTEPKTYIPTLNDEFVKAMGEEDGSPANVEVVTGA
                     THSSHSFIMYAQQLVNAAEKGDTQTIEVDNIVTEK"
     gene            6355..7458
                     /locus_tag="ACG842_001837"
     CDS             6355..7458
                     /locus_tag="ACG842_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HFQ0248372.1"
                     /translation="MRKKLLFSLITLGISFMVLTGCSNSANKTTTESSAATKILKDPY
                     SDEQFLLGTYVRIRVYDEGKESALKPAFDRVKELGDKITINQKGSEIDEVNEQAGIKP
                     VKVSDDVYTLVKRAYEYSQDSQGGFDMAIGAITQLWRIGFDDARKPSQEEIDQALKLV
                     DYHKIELNDKEKTVYLKEKGMIIDLGAIAKGYITDEVVKVLKKQGVTTAIVDLGGNVY
                     VLGHSPRGENQDWTVGIQDPNMARGSVLGSIKERNKTLVTSGIYERYLEVDGKKYHHL
                     FDPKTGYPFDNDIASVTIITDKSIDGDGLSTAVFSMGVKKGLEYVESELNNGTEAIFV
                     TKDDKVYVTDPIKDTFKLSEDSHYTMGDRSELK"
     gene            7471..8424
                     /gene="menA"
                     /locus_tag="ACG842_001838"
     CDS             7471..8424
                     /gene="menA"
                     /locus_tag="ACG842_001838"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HFQ0248373.1"
                     /translation="MTLKIFLEVVEIRTKVASVFPFAVGVLFSIAFFHEIHWLNTFLF
                     FIGMLVFDLATTAINNYMDYEKAHSEVYKYEENVIGRRNLSPVLIRNMILAMIAFVFV
                     IGVILTINTGWLLLLMGFICCFIGVFYTFGPIPLSRMPLGEIFSGVTMGLGIFAMVVY
                     VNTFGSDVYDLTVNFSSGQFALVGNLWAIAAIVLASLPLVFTIANIMLANNLRDLDRD
                     IENHRYTLVFYIGRPAGTVLFQLLMYACYFVILMGLLSGIYEWPILVTFLTLPIVKKH
                     LDQFKQTLPQPISFSYSIKNMVLFNASYVLGLLLTIIMQAI"
     gene            complement(9238..10584)
                     /gene="gor"
                     /locus_tag="ACG842_001839"
     CDS             complement(9238..10584)
                     /gene="gor"
                     /locus_tag="ACG842_001839"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004362 - glutathione-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HFQ0248374.1"
                     /translation="MQIYDYIVIGGGSGGIASANRAGMHGAKVLLIEANELGGTCVNV
                     GCVPKKVMWQASTILETIERDASSYGIQADLKNVDFAGLVERREKYIDFLHGAYQRGL
                     DSNHVEAIKGYATFIDSQTIEVNGETYRAPKILIATGGRASKMTIPGGEYAIDSNGFF
                     ELTELPKRAIVLGAGYIAAELSGVFRGLGSEVMWAYRKERPLRTFDKMLSDNLIQMYQ
                     EAGIKTYAHHIAKEITKNNDEYTVLFENGETLTGDCVLFAGGRVPNTEKLGLENTNVE
                     LDKKGFIKVDKYQNTTDEHIFAVGDVIGKLDLTPVAIAAGRRLSERLFNGKKDSYLDY
                     KLVPTVVFTHPPIATIGLTEEEALEKYGENELKVYRSRFTPMYFALNDYRQKCEMKLI
                     CVGKEERIVGLHAIGVGVDEMLQGFAVAIKMGATKEDFDNTVAIHPTGAEEFVTMR"
     gene            complement(10718..11971)
                     /locus_tag="ACG842_001840"
     CDS             complement(10718..11971)
                     /locus_tag="ACG842_001840"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1901576 - obsolete organic substance
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HFQ0248375.1"
                     /translation="MMKEMTVKRKLKRPEILAPAGTLEKLKTAIHYGADAVYIGGNAY
                     GLRSRAGNFTYEEMAEGVAFAKEHDAKVYVAANMVTHEGNEDGAGEFFRSLRDVGISA
                     VIVSDPALIEICATEAPGLPIHLSTQASATNFETLEFWKEEGLERVVLAREVSMEEVA
                     EIRKNTDTEIEAFIHGAMCISYSGRCTLSNHMSMRDANRGGCSQSCRWKYGLFDMPFG
                     QERNSLTDTGEVEEEFSMSAVDMSMIEHIPELIENGVDSFKIEGRMKSIHYVSTVSNV
                     YKAAVDSYMEDPENYVCKQEWIDELWKVAQRELATGFYYNTPTENEQLFGERRKIPVY
                     KFVGEVLSYDEATQIATIRQRNLFSVGDEIEFYGPGFSHFSQTVEVMHNEEGESIDRA
                     PNPMMILTMPVAQPVKPGDMIRKRK"
     gene            complement(11977..12900)
                     /locus_tag="ACG842_001841"
     CDS             complement(11977..12900)
                     /locus_tag="ACG842_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase U32 family protein"
                     /protein_id="HFQ0248376.1"
                     /translation="MIELITTVETIEQAEQLLAAGADTLYFGEEQFGLRLPASFTREE
                     QRQLVELAHKYGKKANIAVNGIIHPEKMKNVPEYLAFLKEINVDVITVGDTGIIYTMK
                     KNPELRIPYIYDAETLVTSARQINFWAKKGAVGAVLAREVPFEEMKAMEEKLDIPVET
                     LVYGATCIHQSKRPLLQNYYNYTKQDEQKDRERGLFISEPKKEETHYSIYEDSHGTHI
                     FASNDLNLSNELNELYQHHYCTWKLDGLFTPGEKFVNITRLFAEAKKQILEGSWDQEK
                     AEKMTSEISKLHPENRELDTGFFYIDPSAIK"
     gene            complement(13121..15613)
                     /locus_tag="ACG842_001842"
     CDS             complement(13121..15613)
                     /locus_tag="ACG842_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HFQ0248377.1"
                     /translation="MEELFTERAQAVLEIAQEEAKRLKHQSVSSEHVLLALVVEQNGI
                     AGKVLREMDLNETEIYEEIEHLIGYGGIRSYPKGVFLPYSPRMKQVFALASSEVKKTG
                     SQKVGTEHILMSLLKDESIMATRIMINLGISLSKARQVLKQKMGLAGDSAGKGMNRRR
                     NVNVQDKRQTPQGTPTLDSLARDLTKLAKENKLDPVVGRSREVKRLIQILSRRTKNNP
                     VLVGEPGVGKTAIAEGLAQKIILGEVPEDMQEKRLMMLDMGSLVAGTKYRGEFEDRMK
                     KVIDEIYNDGQVILFIDELHTLIGAGGAEGAIDASNILKPALARGELQTIGATTLDEY
                     QKYIEKDSALERRFARIQVDEPTPEEAEVILQGLRTRYEEHHGVEITDEAVRAAVNLS
                     VRYITSRQLPDKAIDLIDESAAKVRLDQTDHLTKSTVIKLEIDELVQEKEAAIQKQDF
                     ENAAQLRRQEKALRKKLQKVSAIEAKQEQGYSDRVTEEDVATVVSEWTGVPLQQLEKK
                     ESERLLELEGLLHERVVGQDEAVKAVSRAIRRARSGLKDPDRPIGSFMFLGPTGVGKT
                     ELAKALSEVMFGSEDALIRVDMSEFMEKYSTSRLIGSPPGYVGYDEGGQLTEKIRQKP
                     YSVILLDEVEKAHPDVFNLLLQVLDDGHLTDSKGRKVDFRNTIMIMTSNIGATQIREE
                     KNVGFNVQDVTKDHKAMQKRILEELKKAFRPEFLNRIDETVVFHSLSKDEIHTIVQIM
                     SKAIIKRLKEQDIQVKITPSAIDVIGKAGFDPEYGARPIRRALQKEIEDRLSEALLGG
                     EIRLGDHVTVGASKGKITLNVRGPKKETVGSL"
     gene            complement(15620..16087)
                     /locus_tag="ACG842_001843"
     CDS             complement(15620..16087)
                     /locus_tag="ACG842_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HFQ0248378.1"
                     /translation="MAHQNTSDLIESYIKKILENSEMIEIRRIEMADLFNCVPSQINY
                     VINTRFTIQRGYLVESKRGGGGYIRIAKVRISDKKQLLEQINQLFDDTISEKNAFAII
                     QKLYEDQIITKKEGNLMLSAIAKNTLNFNEYEDHTRARILRAFLERLSYEDGK"
BASE COUNT         4765 a         3394 c         2887 g         5174 t
ORIGIN      
        1 agcacggaca cgtcagcttc aagcgacagt acggatacat ctaccacaac aagtgatagc
       61 acggacacgt cagcttcaag tgacagtaca gatactacct ctaccacaag tgatagctca
      121 gattcatcta ccactacaag tgatagcacg gacacgtcag cttcaagtga cagtacagat
      181 acatcctcta ccacaagtga tagctcagat tcatcaactt caagcgatag tacagacacg
      241 tcgtctacca caagcgatac acgtcatgca agtgtctcct tctcaaaaac aagttcggat
      301 aacaggacga actttaactt aactaataaa acaagcaatg acaatggaaa caaatcaaaa
      361 agagcacttc ctaaaactgg aagtcaatcg aataattgga taacactcgc aggcgttata
      421 ttacttgtaa tcggtttaag aatcactttt atgagttata agaaatcgaa aaggtaaaaa
      481 aatattcaag ataaaaaaga gcaacgtcgc taacggacgt tgctctttat ttttgctttt
      541 tatcataaat acaagaacta caattcaatt tgtcaaaaaa cacgtagatt tccttctgag
      601 aaatttattt acgtatcgaa cctttcccta ttcagtcttt gcgatccaaa atcagttggg
      661 taagggaaaa gagattctct tttgttcgaa gagaggtggc aggtaatttc tttatttcct
      721 tcaaagcctt ttctgtgtaa gaggccgcta acttcctagc ttcttccacg cctcctgtat
      781 gttcaacgat tttgcgtacg gcgtcgacat cttcttctgt cattgccgct ttcttctcta
      841 aataaggtag tagctttggt ttgttttttt gcaaagagta gatcaacggt aaagaataga
      901 ccccttgacg catgtcttct agtacaggtt tgccgatttc ctggctctct tttgtataat
      961 ctaaaatatc atcgataatc tgaaaggcca tcccgataga aagtccgata tctcctgctt
     1021 tttttgcgaa gcgctcgctt gttccacttt caaaagcgcc aacagaacaa cttaattgga
     1081 acagttcagc tgtcttacct gaaatatttt ccaaatattc ttttacagaa agttcataat
     1141 tatagcgatc atccatctgc cctaattcgc cattcaatac tttttccata ctgcgagaat
     1201 tcaattggag acttttcaag gaagtggagt aatcagacaa tagtttaaaa cagcagacaa
     1261 ataaatagtc tcctgcatag acagaaacga cattaccaaa ctgactacgg atattaggct
     1321 gaccacgtcg cagatccgct tcatcaacga tatcatcatg gatcagtgtg gccgtatgga
     1381 gcatttcgat agaagctgcc aaagcgatgg ctttgtctcg atcttgctct ggtccaaatt
     1441 gtgagaacaa tagctgatag gcaggacgca acagctttcc gccggcatgg atcgtttctt
     1501 tgattgcttg ttcgacaggt ttgtttttca ggcggacagt ttcttccatc cgttttagtg
     1561 tttctgtcaa ttctttactt aaagcaggat agttttccca cattggatgt agcttcataa
     1621 aaaaactcct tgtgtgacga ttattttttg gtaaacattg ggctccattt ttgtttggtt
     1681 ccctgctcat aatctttttg gaatcgttga agtgtctgca catgtcgtaa aagataattc
     1741 gctgcgatcc cgattgcaat ccctgataaa atccctaaaa ctgaaaggac tggcaagtac
     1801 aacatgactg tccatgattg tgcaatgaaa gaagccatca aaagctgtcc gacattatgc
     1861 atgaaaccgc cggccgcact aattccgatg atactgacac gttttggtcc taattgcttg
     1921 accaataaca ttccaaaata actaagcaaa gcaccactca tactataaag gaatgtggaa
     1981 atcgttccgc ctagcaaagt agtcaggaat agtcgtagcc aaagtagtaa gaagctgtct
     2041 cgttttttca tggtaaaaat ggcaacgatc gtgattagat tggctaagcc tagttttgca
     2101 ccaggcgcaa aagaaaaagg ataagggatc atattttcca ataaaccaat gatcactcct
     2161 tgtgcaacaa gtagcgaaat ataaatcatt ttttgtaatt tactcatggt gtgtgaaggt
     2221 ctaataaatc agactgtctt cgtcacctcc atctgttgaa cggacttcga tcactaattt
     2281 atgtggcaaa cagacgatgg tttcgccatt tttcgttgcc cagcctcgac gtacgcagat
     2341 ctggtcatcg caatctgctt cttttatccg tactcgatcg ccatctatct cgatcaagtt
     2401 ataatctccg tgttcatctt tgtaagtata tgtgttgctc tcttctccag ccatcaaagg
     2461 gaatgttttg atttctgtgc catccacacg cagtaccgct tctttttctg ctgttgcggt
     2521 gttttgctga agaccaaaaa tcatgactgg taaaaaagaa agaacagtca aaagcaaaat
     2581 aataaaaaca tcccaaggtt tgagcagact tttttcagca aattctttaa ggctcatttt
     2641 tgttctcctt tttccagatt aactcaccca attttagcat aaaaacaaga ggttgtgaca
     2701 cctgtgtctt gctttgagta ataagaacga agaatagaaa acagctttcc atgttttcta
     2761 attctttggg cttattaccg agaagcagca ggtggaacac cgtttataac aagaggttgt
     2821 gacagaagtg ggcaacttca agaaataaga aggaatttac gaaaattact tttcaagttt
     2881 ttgtgaaatt tcggcttatt tttcgaagaa gctacttctg gaacaccgtt tataacaaga
     2941 ggttgtaaca gaggttgtga cacctgtgtc ttgctcatgg aggcttatct cgggcatctt
     3001 attcctttaa ttttacataa aaaggaggct gtgacggttt gtctcagccc ctttctattg
     3061 aaatcttgca ttcaaataac gttctttatc tatttatttc ttgccgtact gggaacttgg
     3121 tctaccgtac caccaattac ctagtgtacg ttgtagccat ggaattaccc agcgatccaa
     3181 cccaatcgca cgtccggaac cgttcatcaa tgcgaaagca acaaaaatga accaaatgtt
     3241 tacccagtaa aacatgcctg ataagcagaa cgtgatcacc aatacaatcg ttgtcgcact
     3301 gcatagccat gtgaataatc cagcaatcaa tgctaatgcc aaggcaattt caaagaatac
     3361 catgaatttt tggaagaaca ttgctacatc attgtttggc atgatcactt gcatgatctt
     3421 tgtgaaccag tctggtgact tctcaatcac agccattggt tcttcaccgt atgcataact
     3481 caaaccaaaa tgtgcagctt gagtcgctgc ttctccggca cttgcccctg ctccgctggc
     3541 agctgtggtt gcatcaccag cagcagaagc accactggca acatctgtta cattgtattc
     3601 ccatgtccaa gggaacacac tgtttccagt gaaccatgat ccttcaccaa accaagtact
     3661 tggtttgaac acttcaccat tcccaatgat ttttttggaa gcttcaacga accaaactaa
     3721 accatagaaa atacgaagtg gcacactcca taagacattg ccgtaacgag aagaatgtcc
     3781 tcgagtcacg ttgcgatcat ctttgatatg gaaaaactca tgcattaaat attggaacat
     3841 ataataacca gaacgaatat caaagaagta tttcaaattc acgatatgtt tcattacgat
     3901 agctaaaaat ccgcttaaat ggatcttatc aaataggtta gctacccccc atttggcacc
     3961 aatagaaacc ataaatcctt gatagtttcc tttaaattta tgcttttctc cgcctttgat
     4021 cgaagccacg atattcgctg cggctgtgtg tcctgtttgt tcagcagctt gaacgatctg
     4081 cggtgtcggt gttcctggga attcttcata aaagactaaa tcaccaatga tatagatgtt
     4141 tttatcttcg tagccttttg cctgcatata ttcattggcg atcaaccgct gtccgcgtgc
     4201 tgattctaat ccaaagtcag cagcgtcaga agttcctttg acaccagctg tccaaatcag
     4261 tgtgtgagtc ggaatgttgc gtccgtcttt caatttgata tgatcagcag ccacttctac
     4321 gattggtgca ttcaataata attccacgtt gtgcttacgc aaatagcgtt cagcttttgc
     4381 agcatcattt cgatccaaca tattcaagat cgtcggcatt gcctctacga ccatcagtgt
     4441 gatttcgttt ggatctaatt tataatcttt ggctaggcga tttttccagt cgatcagttc
     4501 cccaatcatt tcaataccgg taaatccaga accacagaca acgaaagtca gcatggcttt
     4561 acgcagttca tcatctggtt ccaatgcagc ttttgctact gtcttctcaa tatgttcccg
     4621 aatccttaca gaatcttcaa atgaccaaag ggtgaatccg tgtgctttga caccaggtgt
     4681 accaaagtca ttcggctctc cgcccattcc aaggatcagc tgatcaaaag gataggaacc
     4741 taatttggtc ttgacgattt ttcgttcttt atcgataccg attacagtgt ctgtgacaag
     4801 ttgtacattc ttttgacgag caaacaaatg ctgcagatcg tattggattg cttctggttc
     4861 tacacgtccg ccagctactt catgcaactc ggtcatcata gtatggtacg aatgacgatc
     4921 gatcagagta attgctacat cttctttttt cagtttcttt gccaagtatt ttgtagctga
     4981 aacaccggca tatcctgctc ctacaacgac aatattttgc tttgccattg atcatccgct
     5041 ccttatagac tatctatata atgaatatat gataattctt ttagtttgat acttattaca
     5101 caatctccat atattatata gatgttctgt tcttttgtct aactcctttt ggattttttt
     5161 caaattttaa aataaagcgt tttttcacga aatttgctga aagaaaacgt ttgaaatatt
     5221 acatgggtta tttatcaaaa atagttgact tataggctaa aaagattaga atgtataatg
     5281 aatcacaaag aaaaattgtg gactagaaat ggaatggtgg ggtattaaaa tgaagaaaaa
     5341 acgttttgta actggatttg ccgtattagc attttcagct ctagtgttag gcgcttgcgg
     5401 cgcagatgac aacggaagct caaactcttc ttctgaatca tctacagcac aatcttcgac
     5461 tgcaaaaagc acaacagaat catctgcaaa agttgtagct ggcggagatt tgcaagacgg
     5521 cacatataaa ttagaagaaa aaaattattc aaatggttac cgtgcagtct ttgaaatggt
     5581 tgtaaaagac ggcaaaatca ctgaatcaaa atatgataac gtcaatgaaa acggcgaatc
     5641 aaaaacaaaa gatgcagaat acaacaaaaa catggaagca aaatctggca caaacccaga
     5701 aaaatacatt cctgagttga atgaccaatt cttaaaagca caaagcgcaa gcggtgtgga
     5761 agtcgtaact ggagctactc actcatctga aagcttccaa aactacgcac aacaattgat
     5821 ccaagctgct caagctggta acacagatac aatcgaaatc gacaatggtg ctgatttgaa
     5881 agacggcact tacaagcttg aagaaaagaa ctactctaac ggctaccgtg tgcaattcga
     5941 aatgacagtt gctggcggca aagtaactga atcaaacttt gattatatcg acaaagacgg
     6001 taaatcaaaa caagatgata cagaatataa tgaaaatatg aaagcaaaaa gcggtacaga
     6061 accaaaaact tacatcccta ctctgaacga cgaattcgtc aaagcaatgg gcgaagaaga
     6121 tggctcacct gctaatgtag aagttgttac aggtgctaca cacagctcac attcattcat
     6181 catgtacgca caacaattag tgaatgcagc tgaaaaaggc gacactcaaa caatcgaagt
     6241 agataatatc gtaacagaaa aataatcaca taatcatact aataaaagtt ggagggtggg
     6301 acgatagcca gattaggcag tcgtctcacc tttcaaaatg aagagaggaa aaagatgcgg
     6361 aaaaaactac tattttcctt aatcacactg ggcatctctt ttatggtgct aactggctgc
     6421 agcaactcag caaataaaac aaccactgaa agttcagcag ctaccaagat attgaaagat
     6481 ccatattctg atgaacagtt cttgttaggg acttacgttc gtatccgtgt gtatgatgaa
     6541 ggcaaagaat cagcattgaa acctgccttc gatcgagtaa aagaacttgg tgataaaatc
     6601 acgatcaatc aaaaaggttc tgagatcgat gaagtgaatg agcaagctgg gatcaaacca
     6661 gtaaaagttt ctgatgacgt ctacacgtta gtcaagcggg cctacgaata tagccaagat
     6721 tctcaaggcg gattcgatat ggcgattggt gcaatcacac aattatggcg aatcggtttt
     6781 gatgacgcca gaaaaccttc ccaagaagaa atcgatcagg cattgaaatt agtggattat
     6841 cataagatcg aactaaatga caaagaaaaa actgtttatc taaaagaaaa aggaatgatc
     6901 atcgatttag gtgcaatcgc caaaggttac attacggacg aagtagtgaa agtcttgaaa
     6961 aaacaaggag tgaccactgc catcgttgac ttgggcggta atgtctatgt cttaggccac
     7021 agcccaagag gcgaaaacca agactggaca gtcggcatcc aagatccgaa catggctcgc
     7081 ggatctgttt taggttctat taaagaacgg aacaaaacat tagttacctc tggaatttac
     7141 gagcgttatt tagaagtaga tggcaaaaaa taccatcatt tgtttgatcc aaaaacgggt
     7201 tatccgttcg acaatgatat cgcaagtgtc acgatcataa cagataaatc aattgatggt
     7261 gacgggttgt ctacagcagt cttttcaatg ggtgtgaaaa aaggactgga atacgtcgaa
     7321 agcgaattga ataacgggac ggaagcgatc ttcgtgacaa aagacgataa agtatatgtg
     7381 accgatccaa tcaaagacac cttcaaatta tcggaggact cccattacac gatgggtgac
     7441 cgttctgaac taaaataagg agtacgaacc atgacactaa aaatcttttt agaagtagtt
     7501 gaaatacgaa caaaggtggc tagtgtattt ccttttgcgg taggggtatt attctctatc
     7561 gctttttttc atgagataca ttggctaaac acttttttat tttttattgg aatgctagtt
     7621 tttgatttag cgactaccgc tatcaataat tacatggatt atgaaaaggc tcattctgaa
     7681 gtatataagt atgaagaaaa tgtcattggc cgcagaaatc tatctcctgt attgattcga
     7741 aacatgattt tagctatgat tgcatttgtt tttgttatcg gcgttattct aacgatcaat
     7801 acgggttggc ttttactttt gatgggattt atctgttgtt ttattggagt attctataca
     7861 ttcggaccga ttcctttatc tcgaatgcct ttaggggaaa ttttcagtgg ggtgactatg
     7921 ggccttggaa tttttgccat ggtcgtttat gtcaatacat tcggttcaga tgtctatgat
     7981 ctgacagtca acttttcatc tggacaattt gctttggtag gaaatctttg ggctatagca
     8041 gcaatcgtat tagcttcctt acccttggtg tttacgattg ccaatatcat gttggctaat
     8101 aatttgcggg atcttgaccg tgatattgaa aatcatcgct atacactagt tttttatatc
     8161 ggacgtcctg cgggaacagt cttattccag ctattgatgt atgcttgcta tttcgtgatt
     8221 ttaatggggt tgttgagcgg tatctatgaa tggccgatct tggtcacctt cttgaccttg
     8281 ccaattgtga agaagcattt ggatcaattc aagcaaacgt taccccagcc tatcagtttc
     8341 agctattcca tcaaaaatat ggtcttattc aatgccagtt atgtattggg gctattacta
     8401 acaatcatta tgcaagccat ttaacagcat gaagaggttg tgaaaaaact gccctgcatc
     8461 aagtaataag aacagccaaa taaaaatagc tccttatatt tttcgtttgg ttgggcttat
     8521 taccgcagat gcaagctttt gaacaccgtt tatcaggagg ttgtgaaaaa gtcgcttagt
     8581 ttcgagtatt aagaaaaatt ttgtgaaaat ggctttccac atttttgcat aaatttggct
     8641 taataccgag aaactggctt ttgaacaccg tttatcagag gttgtaaaat cagtgttttg
     8701 tcctgagtat tagagaaaca aatggaaaaa tagttcctca tattttgccg ttttttgagc
     8761 taataccgaa ggacagctga ttgaacacta tttgttcgga aaaagtgact aactatgaca
     8821 caattttttt cacagtccct tttttcactt gatttgattc gtaccagcga acttgacgag
     8881 aactttcttg gtttggtaac tatatggtat tcttttaaca tcctcgtttg cttcttctaa
     8941 tttatttata caaagtcaca cttttttaaa catctgttct tcgatacatt tctatttctt
     9001 ttaaacatcg ataccaatat tttgaactta agagtgcata ccaaatggat tcatttactc
     9061 ttcgtcagtt acgaagataa tcaaaatctc caacttccct acttatttcg ttttgagaat
     9121 tatcgaagca atcttacata aataactaaa aaacaaaaaa ccagaaaatc agtatactga
     9181 ttttctggtt ttacatggtt tgttcaataa aaagctgttg gaactgttaa tattaaatta
     9241 tcgcatcgtt acgaattctt ccgctcctgt tggatggatg gctactgtat tgtcaaagtc
     9301 ttctttagtt gcacccatct tgatagccac ggcaaaacct tgcagcatct catcgacacc
     9361 aacaccaata gcatgtaagc cgacgattct ttcttctttt ccgacacaga tcaatttcat
     9421 ttcgcatttt tggcgataat catttaatgc aaagtacatt ggggtaaagc gtgaacgata
     9481 aactttcaat tcattttcgc catatttttc caatgcttct tcttctgtca aaccaattgt
     9541 tgcaattggt ggatgcgtaa agacgactgt cggaacaagt ttatagtcaa gataagaatc
     9601 tttctttcca ttaaataaac gttctgataa acgtctacca gctgcgattg caacaggagt
     9661 cagatctaat ttaccgatca catcaccaac agcaaaaata tgttcatccg ttgtattttg
     9721 gtatttatct actttgataa accctttttt gtccaattcc acatttgtat tttccagacc
     9781 taatttttcg gtattcggta cacgtccacc ggcaaaaagt acacaatcac cagttaaggt
     9841 ttcaccattt tcaaatagca ccgtatattc gtcattgttt ttagtaattt ctttagcaat
     9901 atgatgtgcg taagttttta ttccagcttc ttggtacatt tggatcaaat tgtccgaaag
     9961 cattttatca aacgttctaa gtggtcgttc ttttcgatat gcccacatga cttcactacc
    10021 taaaccacga aatacacctg aaagttcagc cgcaatgtat ccagcaccta aaacgattgc
    10081 tctcttaggt aactctgtta attcaaaaaa tccatttgaa tcaattgcgt attctccacc
    10141 aggaatggtc attttacttg cacggcctcc agtagcgatc aagattttag gagcacgata
    10201 ggtttcaccg tttacttcga tcgtttgact atcgataaaa gtagcgtaac cttttatagc
    10261 ctcgacatga ttgctatcta atcctctttg ataagcaccg tgtagaaaat caatatattt
    10321 ttctcgtctt tctactagac cggcgaaatc aacatttttt aaatctgctt ggataccgta
    10381 acttgaggca tctcgttcaa ttgtttccaa aatcgtactt gcttgccaca taactttttt
    10441 aggtacacaa ccgacgttga cacatgttcc gcccaattca ttcgcttcaa tcagcaaaac
    10501 ttttgcgcca tgcatccctg ctctgtttgc tgaagcaata ccgccagagc cgccaccaat
    10561 tacgatgtaa tcatatattt gcattttgtt cctccttatt gaagagatca atcaaaacct
    10621 ttatttaacg atcctttttc tacagaaaac aagtgtaaca cttttcttga attttgtatg
    10681 cgaagatgct taaaacaggg atatatcagc tttttcctta tttacgtttt cggatcatat
    10741 ctccaggttt caccggctgt gcgactggca tagttaaaat catcattgga tttggtgctc
    10801 ggtcaatgga ttcgccttct tcattatgca taacttcaac ggtttgactg aaatgactga
    10861 atcctggacc ataaaattcg atttcatctc ctacagaaaa caagtttctc tgacgtatcg
    10921 tagcaatctg tgttgcctca tcataagata atacttcacc gacaaattta tatactggga
    10981 ttttacgacg ttcaccgaat aattgttcgt tttcagttgg tgtattgtag taaaaacctg
    11041 tagctaattc tcgttgtgct actttccata actcgtcgat ccattcttgc ttgcagacat
    11101 agttttcagg atcttccata taactgtcta cagccgcttt atagacattt gacaccgtag
    11161 atacataatg gatcgatttc attcttcctt cgatcttgaa gctgtctacg ccgttttcaa
    11221 tcaattctgg aatatgttcg atcattgaca tatccactgc actcatcgag aattcttctt
    11281 ccacttcgcc tgtatcagtt aaactgtttc gttcttgacc aaaaggcata tcgaacaatc
    11341 catacttcca gcgacaagat tgagaacatc cgccacggtt tgcatcccgc atcgacatat
    11401 ggttggacaa tgtgcatcga ccagaataag agatacacat ggcaccatgg ataaatgctt
    11461 cgatttcggt atccgtattt ttacgaatct ctgccacttc ctccattgaa acttcacgag
    11521 ctaaaacgac acgttccaac ccttcttctt tccagaattc caatgtctca aaattagttg
    11581 ctgaagcttg agtggataaa tggatcggca gacctggggc ttctgtcgca caaatttcga
    11641 tcaaagctgg atcagaaaca atcactgcag aaataccgac atcacgcaaa ctgcggaaaa
    11701 attcccctgc tccatcttca tttccttcat gggtgaccat attagcagca acgtatacct
    11761 tcgcatcatg ttcttttgca aaagcaactc cttcagccat ttcttcataa gtaaaattac
    11821 ctgcacggct tcttaaacca tatgcattcc cgccaatata tactgcatct gctccataat
    11881 ggatcgcggt ttttaacttt tccagtgttc ctgctggcgc taggatttct ggtcgtttca
    11941 attttctttt tactgtcatt tctttcatca tactccttat ttgatagcag aaggatcaat
    12001 ataaaagaat cctgtatcta attcacggtt ttcaggatgc aatttactga tttcgctagt
    12061 cattttttcc gctttttctt gatcccagct accttctaaa atttgttttt ttgcttcggc
    12121 gaatagtcga gtgatgttta cgaatttttc gcctggagta aataatccgt ccaatttcca
    12181 tgtacagtaa tgatgctgat aaagttcatt caattcattc gagagattca gatcattgct
    12241 ggcaaaaata tgtgtgccat gactatcttc ataaatagaa taatgtgtct cttctttttt
    12301 aggttccgag ataaaaagac ctctttcacg atctttttgt tcgtcttgtt tcgtatagtt
    12361 atagtaattc tgtagcagtg gacgtttgga ttgatggatg catgtagcac catacacaag
    12421 ggtttctaca ggaatatcga gtttttcttc catcgccttc atctcttcaa aaggaacttc
    12481 acgagcaagt actgctccaa ctgcgccttt tttcgcccag aaattgattt gacgtgcgct
    12541 cgttactaat gtttcagcat cataaatata cggtatacgc aattcaggat tttttttcat
    12601 cgtataaata atacctgtat caccaactgt gatcacatct acgtttattt cttttaaaaa
    12661 agccaaatat tcaggtacat ttttcatttt ttcaggatga atgatcccat ttacagcaat
    12721 attcgctttt ttaccatact tgtgtgctaa ttctaccaat tgtcgctgtt cttctcgtgt
    12781 aaatgaagca ggcagacgca atccaaattg ctcttcaccg aaatatagtg tatctgcacc
    12841 tgcagcaagc aactgttctg cttgttcaat agtttctact gtggttatga gttcgatcat
    12901 aattttctcc tcctacaact tcataatagt agcatagctc taaaaacttt ccaatacttc
    12961 ttctatttaa agtcggattt ttgaaaatta tttcacaaaa aatcaaaata aagttcgata
    13021 tattctgtcc aaatataaaa agagattgtg acaaaaatct tgtcacaacc tcttaatcaa
    13081 gtcttactat tataaaaatt cagctacaca ggccaagtaa ctatagggaa cctactgttt
    13141 cctttttagg tccgcgaaca ttcaatgtga tcttaccttt tgaagcacct actgttacgt
    13201 ggtcacctaa acgaatttct ccgccaagca aagcctcgct caatcgatct tcaatttctt
    13261 tttgaagtgc gcgacggatt ggacgtgcac catattcagg atcaaatcca gctttcccga
    13321 tcacatcgat tgcagatggt gtgattttca cttgaatatc ttgttctttc aatcgtttga
    13381 taatcgcttt gctcatgatt tggacgattg tatggatttc atctttactc aatgaatgga
    13441 agacgacagt ctcatcgatt cgattcaaga attcaggtcg gaaagctttc tttaactctt
    13501 ctaagatacg tttttgcatg gctttgtgat ctttagtaac atcttggaca ttgaatccga
    13561 catttttttc ttctcgaatt tgtgtcgctc cgatatttga agtcataatc atgattgtat
    13621 ttctaaagtc cacttttcta cctttagaat ctgttaaatg accatcatcc aatacttgca
    13681 gaagtaaatt gaaaacgtct ggatgtgctt tctccacttc atctaagaga atcactgaat
    13741 atggtttttg acgaattttt tctgtcaact gtccaccttc atcataccct acgtatcctg
    13801 gaggagaccc gattaatcgg cttgtactgt atttttccat aaattctgac atatcaacac
    13861 ggatcagggc atcttcactt ccaaacatga cttctgaaag tgctttggcc aattctgttt
    13921 ttccgacacc agttggtcca aggaacatga atgaaccaat tggacgatca ggatctttca
    13981 atccgcttcg tgcacggcgg atcgcacgtg aaactgcttt gacagcctca tcttgaccaa
    14041 cgacacgttc atgcagaagt ccttccagtt ctaacaagcg ttcgctttct tttttctcca
    14101 actgctgtag agggacacca gtccactccg atacaactgt agcaacatct tcttctgtta
    14161 cacgatcaga ataaccttgc tcctgttttg cttcaattgc agaaactttt tgcagctttt
    14221 tacgtagtgc tttttcttgt ctgcgtaatt gagcagcatt ttcaaagtct tgtttttgga
    14281 tcgctgcttc cttttcttgt actagttcat cgatttctag tttgatcaca gttgattttg
    14341 ttaagtgatc ggtttgatcc aaacggactt tcgctgctga ttcatcgatc aaatcaattg
    14401 ctttatcggg tagttgacga gaagtgatat aacgaacaga aagattgact gctgctcgta
    14461 cagcttcatc tgtgatctcc actccatggt gctcttcata tcttgtacgt aatccttgaa
    14521 gaatcacttc tgcttcttca ggagttggct catctacttg aatgcgagca aaacgtcgct
    14581 ctaatgcaga atctttttcg atatattttt gatattcatc tagggtcgtt gctccgatcg
    14641 tttgtaattc tcctcgagct aacgccggtt tcaagatatt cgaagcatca attgctcctt
    14701 ctgctccacc tgcaccaatt aaggtatgca attcatcaat gaataagatt acttgaccat
    14761 cattatagat ttcgtcgatg acctttttca ttcgatcttc aaattcaccg cgatatttcg
    14821 taccagcaac caaagatccc atatcaagca tcatcaggcg cttttcttgc atgtcttctg
    14881 gcacttcacc taaaatgatt ttttgagcta acccttcagc aatggctgtt ttgcctacac
    14941 caggttcacc caccagtaca gggttattct ttgtccgtcg gctaagaatc tggataagcc
    15001 gtttcacttc tctcgaacga ccaacaactg ggtccagctt gttttctttt gctaatttcg
    15061 tcaaatcacg tgctaatgaa tctagcgtag gtgttccttg cggtgtttgc cgtttatctt
    15121 gtacatttac atttctacga cgattcattc cttttccagc agaatcacca gctagcccca
    15181 ttttttgttt cagaacttga cgggcttttg aaaggctgat acctaagttg atcatgattc
    15241 gagtagccat gatactttca tcttttaata agctcatcaa aatatgttct gttccaactt
    15301 tttgagaacc agttttcttc acttcgctgc tagctaaagc aaaaacttgt ttcattcttg
    15361 gtgaatatgg aagaaagact cctttagggt aagaacggat accaccataa ccaatcagat
    15421 gttcaatttc ttcataaatt tccgtttcat tcaaatccat ttcacgtaat acttttccag
    15481 caattccatt ttgctcgacc actaaggcta gtaaaacatg ttctgaacta actgattgat
    15541 gtttcaaccg ttttgcttct tcttgtgcaa tttctaatac tgcttgggct ctttcagtaa
    15601 aaagttcttc catctaatct cacttcccat cttcataact taaacgttcc agaaatgctc
    15661 ggagaatccg agcacgtgta tgatcttcat attcattgaa attcaatgtg ttttttgcaa
    15721 ttgcactcaa catcaagttt ccttcttttt ttgtaattat ttggtcttca taaagttttt
    15781 gaataatagc aaaagcattt ttctcactga ttgtatcatc aaataactga tttatctgct
    15841 caagtaattg ttttttatct gaaatcctta cttttgcaat tcgtatataa ccaccaccac
    15901 cacgtttgct ttctactaga taaccacgtt ggatggtaaa acgggtatta atcacatagt
    15961 tgatttgtga aggaacacag ttaaagaggt ctgccatttc aatccttcga atctcgatca
    16021 tttcactatt ttccagaatt ttctttatat atgattcaat taaatccgag gtattctgat
    16081 gtgccatctt attcattccc tttgactaat attgacctta attataacga atttgataat
    16141 agaaagaaag aagaacgctt tttgttaaat aaatccttta gaatcaacaa aaaaaaatac
    16201 ggaatatact attccgtatc
//
LOCUS       DAZKLM010000060        15742 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3764
            SAMEA115706913-rid22582563.denovo.062, whole genome shotgun
            sequence.
ACCESSION   DAZKLM010000060 DAZKLM010000000
VERSION     DAZKLM010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706913
            Sequence Read Archive: ERR13196647
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15742)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15742)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287823.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287823.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,004
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,849
            CDSs (with protein)               :: 2,849
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 41 of 96
            Pseudo Genes (incomplete)         :: 52 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 15 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15742
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706913-rid22582563.denovo.062"
                     /isolate="VRE3764"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(24..722)
                     /locus_tag="ACG842_001844"
     CDS             complement(24..722)
                     /locus_tag="ACG842_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HFQ0248379.1"
                     /translation="MANYELNIAHLYGNLMNTYGDNGNLLMLKYIAEKMGVSCHTEIV
                     SIHEPFDADKYDLVFFGGGQDFEQLIISKDIQKKKDSLINYIENDGVMLAICGGYQLL
                     GHYYMGANGEKIQGISALDHYTLSQENNRFIGDIVIHNEEFNETYYGFENHNGRTFLG
                     EGERPLGKVVQGQGNNGEDQSEGVIYRNVFGSYFHGPILARNENLAARLIRLALEQRY
                     GKELDLPYLEGQTA"
     gene            complement(715..2067)
                     /locus_tag="ACG842_001845"
     CDS             complement(715..2067)
                     /locus_tag="ACG842_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurT ligase domain-containing protein"
                     /protein_id="HFQ0248380.1"
                     /translation="MGIRSHFAIAVGKTSQWALKTFFKGGSSLPGKLALKVDPHILDS
                     LAKDYQVVVVTGTNGKTLTTALTVNILRQQFDEVLTNPTGANMVQGIVSTFLQAKPKK
                     GQKKFAVLEIDEASLSKVTEYMKPELFLFTNIFRDQMDRYGEIYTTYKMIVDGAAKSP
                     NATIISNGDSPIFNSIETVNPRKYYGFDHEEDREQMAHYNTDGVLCPNCHHILHYKMI
                     TYANLGKYYCPNCDFKRPELDYRLTDMVRMDNVSADFVIDGHEYGIEVGGMYNVYNAL
                     AATAVAEYFNVDPDKIKQGLGYDEKVFGRQEVININGKKCTLVLVKNPVGINQVIDMI
                     GLAPYPFSLVALLNANYADGIDVSWIWDGNFENFADMDIPAVISGGDRHTDMTLRLKV
                     AGIPENKLTETKELPEVIEKIKELPTEHVYILATYTAVLQLRKELAAQGFIKGGMNRG
                     "
     gene            complement(2290..3690)
                     /locus_tag="ACG842_001846"
     CDS             complement(2290..3690)
                     /locus_tag="ACG842_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HFQ0248381.1"
                     /translation="MHVDLFFRRLQRRGQKFAANHFSSIQIIVFYYILMTVLSLVLFY
                     MPIFREPDSHVSFVDMLFMAISTVSVTGLSTFDINSVFNDNGIILLEILFQVGGLGIM
                     MISTAFVIFSKRRITLKQRQLIMTDMNQPRLSGIVRLIRITFAILIWFQLLFGTFFSI
                     YFYYRGYFDRWRDAVFYGFYQAISAVTNSGFDVTGDSIKPFAHDYFFLILIMFLIFVG
                     GIGFPVLMECREWLLYKRSNAKLPFRFSLFTKLAVLAFVILFVSGTVLIYLLEKDHLF
                     QDSNMSVKWINSMFYSITTRNAGLQIHDLGDFQITTLIIFSLLMFIGCSPSSVGGGIR
                     TTTVAIIGLYLYSFLKSEDNINIFGRRIDQDDVRKSVVVFMLSLGMCFFCIVFLSATE
                     EQTLISIIIEVTSAFGTTGLSLGITGDLSVVGKITIATLMFIGRIGMLYTLMLFVPKE
                     TRDLGYEYPSEKIIIG"
     gene            3851..4588
                     /locus_tag="ACG842_001847"
     CDS             3851..4588
                     /locus_tag="ACG842_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HFQ0248382.1"
                     /translation="MINAQQIVEKMKNQKINYDTVLKKMIMQWEKEEVKPKILIHTCC
                     APCSTHTLEFMSQYAEITLFFSNSNIHPKSEYLRRLHEQKRFVDQFNEKTDHHVALIV
                     DEYRPNEFTKMVLTNHLENEKEGGARCSACFNMRLDLVAQKAQELGYDYFGSALTISP
                     KKNAALINQIGMDIQKIYGTQYLPSDFKKNKGYERSLQMCKEYDIYRQCYCGCVFAAK
                     QQGCDLKEINREAKAYLKTSTVVFEEN"
     gene            4713..5192
                     /locus_tag="ACG842_001848"
     CDS             4713..5192
                     /locus_tag="ACG842_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A-agglutinin attachment subunit precursor"
                     /protein_id="HFQ0248383.1"
                     /translation="MRKVAPILKPIAVLTISTIVATTTGIVNSSSYRAPSSSSSTVEE
                     TTPTTSVSTSEPTTTESLNETTTTSTWDDTTTSSSYDETYTTDSSSETSNTVPDGTPS
                     DSTNPSTSPDTSASSETIPSSTTVDDNTQNDDAARTAQQNQDSGNTQTYNQQAGDSQ"
     gene            5189..5956
                     /locus_tag="ACG842_001849"
     CDS             5189..5956
                     /locus_tag="ACG842_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6681 family protein"
                     /protein_id="HFQ0248384.1"
                     /translation="MTFLLDLINGVHKFLGYLDISPKYLNRGYTILSVIPTLYLLRIV
                     YGLWQNQNYLQFFLYGLAFLVLVYFTVLNVFYYFLDKNTKADVTQLFVKYLPDEAFNI
                     QTEESIDNGNSIDKVNTQEVMVSYDEDYQLKLAENMRYLIGNGEIKTNELGRMDGFLV
                     DRNTLYPYYYVKKTGEKEYVLNIGRSYSDMEKVGTIRLDSTEDTLEPVGLFIVGGDFV
                     KDGLRYHEPYRLKLIAKKAQSQEEPVVYSRSQRRRHS"
     gene            6186..6755
                     /gene="lepB"
                     /locus_tag="ACG842_001850"
     CDS             6186..6755
                     /gene="lepB"
                     /locus_tag="ACG842_001850"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HFQ0248385.1"
                     /translation="MKEFLKNWGILILVLAAILLARVYVFTPVTVNGHSMDPTLSDGQ
                     RLISSKISNYERMDIITTKEPGDEERMIVKRIIGMPGDTVKMENDQLTINGKKYDEPY
                     LDEFKKEFSEDKLQGEYAYSSGFQAQAESSSTFTSDFEYTVPKGKYLVLGDNRLISKD
                     SRMFGLVDKDMIQGKVVFRYWPLSEIKIM"
     gene            complement(6859..7461)
                     /locus_tag="ACG842_001851"
     CDS             complement(6859..7461)
                     /locus_tag="ACG842_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HFQ0248386.1"
                     /translation="MTKNIFDSIANHYDSPDRQALAKIIREELTTYLPRDSHQKVLLD
                     YGGGTGLVSLPLAERFKELIIADASETMLKMAEEKIQAADLKNVRTIHADASVEFPAV
                     QANLILLSLVLLHIPDTENILTKLYEILAPGGQLIIVDFDKNEQISHPKVHNGFSQEE
                     LNNRLEKTGFVSATSHTFHRGEKLFMNKNASLFLSISQKA"
     gene            7567..7797
                     /locus_tag="ACG842_001852"
     CDS             7567..7797
                     /locus_tag="ACG842_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0248387.1"
                     /translation="MPISFVKDREEKGKCVREILLDLPEWFGLPESTEKYIEESSKLP
                     LWCEKRKEEYLGFITLSQTSEDTAEIYSIVWE"
     gene            7788..8036
                     /locus_tag="ACG842_001853"
     CDS             7788..8036
                     /locus_tag="ACG842_001853"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0248388.1"
                     /translation="MGIKKDYHHQGLGTKLFKEAEGYAAKHYKYLQVKTVDEGHYSIY
                     DQTICFYESLGFSRLEVFPNLWDEWNPCLVLVKKLEQK"
     gene            8142..8726
                     /locus_tag="ACG842_001854"
     CDS             8142..8726
                     /locus_tag="ACG842_001854"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004797 - thymidine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HFQ0248389.1"
                     /translation="MAQLFFKYGAMNSGKTIEILKVAHNYEEQNKPVVLMTSGIDTRD
                     GLGVVSSRIGLKRNAMPIFEETDVFDVIQKMESKPYCVLIDEAQFLKKKHVIAFTRIV
                     DELDIPVMAFGLKNDFRNELFEGSKYLLLYADKIEEMKTICWFCHKKAIMNLHYIDGN
                     PVYEGDQVQIGGNEAYYPVCRKHYFHPSMITEGE"
     gene            8729..9802
                     /gene="prfA"
                     /locus_tag="ACG842_001855"
     CDS             8729..9802
                     /gene="prfA"
                     /locus_tag="ACG842_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HFQ0248390.1"
                     /translation="MYDQLQAIEDRYEELGELLSDPEVISDTKRFMQLSKEEANTRET
                     VEVYRRYKEVVQGITDTEELLGEKLDDEMAELAKEELSELKKEKEVLEEKIKILLLPK
                     DPNDDKNIIMEIRGAAGGDEAALFAGDLFNMYQKYAEAQGWKTEVMEANVTGIGGYKE
                     VIMMITGDNVYSKLKYESGAHRVQRVPSTESQGRIHTSTATVVVMPEAEEVEIDIEDK
                     DIRTDIYHASGAGGQHVNKTASAVRLTHLPTGIVVAMQDERSQIKNREKAMKILRARV
                     YDKIQQEAQSEYDANRKSAVGTGDRSERIRTYNFPQNRVTDHRIGLTIQKLDQILAGK
                     LDEIVDALIMYDQTSKLEAMQNG"
     gene            9795..10634
                     /gene="prmC"
                     /locus_tag="ACG842_001856"
     CDS             9795..10634
                     /gene="prmC"
                     /locus_tag="ACG842_001856"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HFQ0248391.1"
                     /translation="MGKMTYREVLSRASSFLEDHGKEAYSIQFLFLERKQWKKLDWLL
                     HMNEEISEEEQRLIETDLRLLLADHPPQYLLGYADFYDHRLKVTEATLIPRPETEELV
                     EWCLDETPGVPLEVIDIGTGTGAIAISLKAARKNWHISAVDLSEEALEVAKENAQKEG
                     TKISFYHGDTLGPVMDQQFDVIISNPPYISRNEWELMDESVRSFEPKMALFAENDGLA
                     IYEKIAVEAPSVLKSNGKIFLEIGFRQGEAVKKIFQQAFPDKKVAIKKDLFGNERMIQ
                     VYG"
     gene            10627..10806
                     /locus_tag="ACG842_001857"
     CDS             10627..10806
                     /locus_tag="ACG842_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0248392.1"
                     /translation="MVEVVDEKFFICFFIIKKHLFDKDVNDFLVYQQVYPLLTNRLST
                     NSGKLEDKLRQNKNV"
     gene            10828..11853
                     /locus_tag="ACG842_001858"
     CDS             10828..11853
                     /locus_tag="ACG842_001858"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HFQ0248393.1"
                     /translation="METKRYDETELQEAAEALKEGELVAFPTETVYGLGANALLPNAV
                     KKVFSVKGRPQDNPLIVHVASFEQVKEYVDNFHPAAEKIVKNFWPGPLTLIFKIKKDT
                     LPSVVTGGLSTAAFRIPDNKKTLEVIELSGVPLVGPSANTSGKPSPTTADHVYHDLQG
                     KITGIIDDGATRIGVESTVLDLSDPTAMPMILRPGAVTKEQIEAVIESPVAIDQHLVK
                     ENETPKAPGMKYKHYSPDTRVLMVRDGDWSTAVQWAKNKKIRAGVIASPEIADQVRTD
                     TAAVYMYNDNSVEAAAKGLFAGLRGLDEPTLGLDLIFVQVYPETGLGNAYMNRLKKAA
                     GQNYFEK"
     gene            12028..13272
                     /gene="glyA"
                     /locus_tag="ACG842_001859"
     CDS             12028..13272
                     /gene="glyA"
                     /locus_tag="ACG842_001859"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HFQ0248394.1"
                     /translation="MVDYKTFDPDLWAAIAKEEERQEHNLELIASENFVSEAVMAAQG
                     SILTNKYAEGYPGHRYYGGCEFVDIVENLAIDRAKELFGAKFANVQPHSGSQANTAAY
                     LALVEPGDTILGMDLSAGGHLTHGSPVNFSGKTYHFVAYGVDPTTEVIDYNVVRILAR
                     KHQPKLIVAGASAYGRTIDFAKFREIADEVGAKLMVDMAHIAGLVAAGLHPNPVPYAD
                     ITTTTTHKTLRGPRGGMILTNDEALAKKINSAVFPGIQGGPLEHVIAGKAVAFKEALD
                     PAFKEYSEQIIANAKAMVKVFNQAIGTRVISGATDNHLMLIDVRELGINGKEAESILD
                     SVNITVNKNSIPFETLSPFKTSGIRIGTPAITTRGFKEEDAVKVAELVVKALQAKDDN
                     AQLDEVKTGVRELTEKFPLHKK"
     gene            13425..14372
                     /locus_tag="ACG842_001860"
     CDS             13425..14372
                     /locus_tag="ACG842_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase fold domain-containing
                     protein"
                     /protein_id="HFQ0248395.1"
                     /translation="MTDRISLEKEAVAFSDANAPHPRIYELAPEEGRVLLEEVQESPV
                     NKYSVDIEDLMVDTGEWGKLNIRLVRPEGNMDKLPVIFYIHGAGWVFGSAQTHDKLIR
                     ELAVRTNSVVVFPEYSRSPEAKYPTAIEQNYAVLQQLNKLAKSKNLDLENLIVAGDSV
                     GGNMATVMTILTKERKGLPISKQLLYYPVTNADFDTDSYHEFGENYFLTREGMMWFWD
                     QYTTNEEERAEITASPLRANINELNDLPSAMILTGEADVLRDEGEAYARKLRDAGVPV
                     TQVRFQGIIHDFVMVNAMDQTKATRAAMALSTSWIKKKE"
     gene            14506..15135
                     /gene="upp"
                     /locus_tag="ACG842_001861"
     CDS             14506..15135
                     /gene="upp"
                     /locus_tag="ACG842_001861"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013774406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HFQ0248396.1"
                     /translation="MGKFQVIDHPLIQHKLTIIRDKNCGTKVFREVVDEIAMLMAYEV
                     SRDMPLEDIVIETPITETTQKTLSGKKVAIIPILRAGIGMVDGILELIPAAKVGHIGL
                     YRDEETLEPHEYFVKLPEDIDARQLFVVDPMLATGGSAIMAIDALKERGASNIKFVCL
                     VAAPEGVKALQEAHPDIDIYTASLDEKLNEHGYIVPGLGDAGDRLFGTK"
     gene            complement(15274..15516)
                     /locus_tag="ACG842_001862"
     CDS             complement(15274..15516)
                     /locus_tag="ACG842_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0248397.1"
                     /translation="MKMATFGKFDTTIVPSEIGEKFKINTHVMFTIHGKSQTGTISKQ
                     LKNSAVVEIDETAENANLMTQSKGVVIVNYKELKKI"
     gene            15637..15709
                     /locus_tag="ACG842_001863"
     tRNA            15637..15709
                     /locus_tag="ACG842_001863"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:15670..15672,aa:Thr,seq:cgt)
BASE COUNT         5349 a         2823 c         3195 g         4375 t
ORIGIN      
        1 tcacattccc ctttttcgaa taactaggct gtttgtcctt caagataggg cagatccaat
       61 tctttaccgt aacgttgttc taaagctagg cggatcaaac gagctgccag gttctcattt
      121 cttgcaagga taggtccatg gaaataagaa ccgaagacat ttcggtaaat gacaccttca
      181 gattggtctt caccattatt tccttgacct tgtaccactt tccccaaagg tcgttcacct
      241 tcacctaaga aagtccgacc attgtggttt tcaaatccat aataagtttc attgaattct
      301 tcgttatgga taacgatatc accgataaac cggttatttt cctgacttaa cgtgtagtga
      361 tccagtgcac tgattccttg gatcttttct ccattagctc ccatgtagta gtgacctagc
      421 aattggtagc caccacaaat ggctaacatg acgccatcat tttcgatata gttgatcaaa
      481 gaatctttct ttttctggat gtcttttgaa atgattaatt gctcaaagtc ttgaccaccg
      541 ccaaagaaaa ccaagtcata tttatcagca tcaaaaggtt catgtatact aacgatctcc
      601 gtatggcaag aaacccccat tttttctgct atatatttca acatcaaaag gtttccgtta
      661 tcaccatatg tgttcatcaa atttccataa aggtgggcga tattgagttc ataattagcc
      721 acggttcatt cctcctttga taaatccttg agcagctagt tctttacgaa gctgcaatac
      781 agcagtgtaa gtagctaaaa tatagacatg ctcagttggc aattctttga ttttttcgat
      841 tacttctggt aattcttttg tttctgtcaa tttattttct ggaatcccgg caacttttag
      901 acgtaaagtc atatctgtat gacggtctcc tccagaaata acagctggga tatccatatc
      961 ggcaaagttt tcaaaattac catcccaaat ccagctgaca tcgattccgt ctgcgtaatt
     1021 ggcatttaat aaagccacta aagaaaatgg ataaggagct agaccaatca tatcgattac
     1081 ttgattgata cctacaggat ttttgacaag tactaatgta cattttttcc cattgatatt
     1141 gatgacttct tgtcggccga atactttctc gtcatatcct aaaccttgtt tgattttatc
     1201 aggatcgaca ttgaagtact ctgccactgc agtagctgct aaagcattgt agacattata
     1261 cataccgcca acttcgattc cgtattcatg accatcgatc acgaaatctg ctgacacatt
     1321 gtccatccgt accatatcag ttaatcgata atccaattca ggacgtttga agtcacaatt
     1381 tggacagtaa tatttcccta agtttgcata agtgatcatt ttataatgca agatgtgatg
     1441 acaatttgga cataagacac cgtctgtatt gtaatgagcc atttgttcac gatcttcttc
     1501 atggtcaaag ccataatatt ttctaggatt gactgtttcg atagaattga agattggtga
     1561 atcaccgtta ctgataatcg ttgcatttgg tgactttgcg gcaccgtcta caatcatttt
     1621 ataggtcgta tagatttctc cataccgatc catttgatca cggaaaatat ttgtaaataa
     1681 gaacagttct ggtttcatat attctgtgac tttgctcaaa cttgcttcat ctatttctag
     1741 gacagcaaac tttttctgtc ctttttttgg ttttgcctgc aagaaagtcg atacaattcc
     1801 ctgtaccata ttcgcaccag tagggttggt tagaacttca tcgaattgct ggcgcaaaat
     1861 attgactgtt aaagcggtag tcaatgtttt accgtttgtt ccggtgacaa cgacgacttg
     1921 gtagtctttt gccaaactat ctagtatatg tggatcgact ttcagcgcta gtttacctgg
     1981 taaacttgat ccgcctttaa aaaaagtttt taatgcccat tgcgacgttt ttccaactgc
     2041 aatcgcaaaa tgactgcgta ttcccattat gtaacctcca aaaacgattt gctgacgaat
     2101 aaattcaact aatcatacca tatgttgaaa aaaaggttaa attgcttttc ttattcttaa
     2161 gaaaaggtta cggctgaaag tgcatgaaat cggacaaaat cgcatgattt aacaaaaaaa
     2221 cagcttgaaa caaaatgatt gtttcaagct gctgtgattt tattattccc tcttacctat
     2281 attcccattt taaccgataa taattttctc gcttggatat tcatagccta gatctcgtgt
     2341 ttcttttggc acgaataaca tcaatgtata tagcattccg atccgcccaa taaacatcag
     2401 tgtagcaatc gtgattttgc ccacaacaga caagtctccg gtaatcccta aagataaacc
     2461 agttgttcca aacgcagaag ttacttcgat gatgatcgag atcagcgttt gttcttctgt
     2521 agcagataaa aaaacgatac aaaagaaaca cataccaaga gaaagcataa agacaacgac
     2581 tgattttcgc acatcatctt gatcgattct ccgtccaaaa atattgatgt tatcttcact
     2641 tttgaggaag gaatatagat acaaaccgat gattgctaca gtagttgttc gaatcccgcc
     2701 gcctacagaa ctaggactgc atccgataaa catcagtaaa gaaaaaatga tcagtgttgt
     2761 gatttgaaaa tcacccaaat cgtgaatctg cagaccagca ttacgagtcg taatcgaata
     2821 aaacatcgaa ttgatccatt tgacactcat attggaatct tggaataaat ggtccttttc
     2881 caatagataa atcaagacag ttcccgaaac aaacaagata acaaaagcta acacagctaa
     2941 tttggtaaac aaggaaaaac gaaacggaag cttcgcattg gaacgtttgt ataatagcca
     3001 ttcccggcac tccatcaata caggaaaacc aattcctccg acaaaaatca aaaacatgat
     3061 caggatcaag aaaaaatagt catgtgcaaa gggcttgatg gaatccccag tgacgtcaaa
     3121 cccagagttc gtgacagccg atatcgcctg ataaaaaccg taaaaaacgg catcacgcca
     3181 acgatcaaaa tagcctctgt aataaaaata aatagaaaag aatgtcccaa aaagtaattg
     3241 aaaccagatt aaaatagcaa aagtgatccg aatcagtcgg acgataccag acaaacgcgg
     3301 ttgattcata tccgtcatga tcagctgtcg ctgtttcagt gtgatccgtc gtttagaaaa
     3361 aatgacaaaa gcagtagaaa tcatcatgat ccccaaacca ccaacttgaa ataatatttc
     3421 cagcagtata ataccgttat cattgaacac agaattgata tcaaaagtcg atagtccagt
     3481 cacactcact gtactgattg ccatgaacag catgtccaca aaagaaacat ggctatctgg
     3541 ttcgcgaaaa atcggcatat aaaatagaac gagtgataag accgtcatca atatataata
     3601 aaaaacgatg atttggatcg atgagaaatg attggcagca aacttctgtc ctcgccgctg
     3661 cagccttcga aagaataaat caacatgcac agtactatcc tcctgacaat agtcgtttct
     3721 accagtatac aaggggaatt aggaatactc aaagaaatta tcaaacagat aacaatacag
     3781 ctttcgatta aaatagaaat ggcttataat aaggaaaatc ctagaaaaaa gaaggaggag
     3841 gaataacaaa ttgattaatg ctcaacaaat cgtagaaaaa atgaaaaatc aaaaaataaa
     3901 ttatgataca gttctgaaaa aaatgatcat gcaatgggag aaagaagaag taaagcctaa
     3961 gatattaata catacatgct gtgctccttg cagtacgcac acattggaat tcatgtctca
     4021 atatgcagag atcacgttat ttttttcaaa ttcgaatatt catcctaaaa gtgagtattt
     4081 gcgaagatta catgaacaaa aacgatttgt agaccaattt aatgagaaaa ctgaccatca
     4141 tgttgcgcta atcgttgatg aataccgtcc aaatgaattc accaaaatgg ttttaacaaa
     4201 ccatcttgaa aacgaaaaag aaggtggtgc tcgctgcagt gcatgcttca atatgcgatt
     4261 agatcttgtt gcgcaaaaag cgcaagaact aggatacgat tactttggaa gtgccctgac
     4321 catctcgcca aagaaaaatg cagctttgat caatcagatc ggaatggata tccaaaagat
     4381 ttacgggaca caatacttgc caagtgattt caagaaaaac aaaggctatg aacgctcttt
     4441 gcaaatgtgc aaagagtatg atatttatcg ccaatgttat tgtggttgcg tgttcgctgc
     4501 caagcagcaa ggatgcgatc tgaaagaaat caatcgggaa gcaaaagctt acttaaaaac
     4561 atcaaccgta gtttttgagg aaaattaaag aaatctttcg gaaaaacgta agaaaaaaat
     4621 aaagaattta tggtagaaat aaggtagact gtataatttg tccgtagtaa agtaaggaaa
     4681 caagaataaa tttcaatgaa ggaagagata agatgcgaaa agtagcacct attttgaagc
     4741 caatcgctgt attaacaatc tcgacgattg tcgcgactac tacaggtatt gtgaatagtt
     4801 ccagctaccg tgcaccaagt tcaagttcta gtactgtaga agaaacaaca ccaactactt
     4861 cagtctcgac atctgaacca acaacaacgg aaagtttgaa tgaaaccact acgacaagta
     4921 catgggacga tacaacaact agttcgtcgt atgatgagac atatacaact gacagctcaa
     4981 gcgaaacaag taatacggta ccagatggca ctccatcgga ttcaaccaat ccaagtacaa
     5041 gtccggatac aagtgctagc tccgaaacga taccaagcag tacgactgta gatgataaca
     5101 cgcaaaatga tgatgcagca cgtacagcac agcaaaacca agattcagga aatacccaaa
     5161 cgtataacca gcaagcaggt gattctcaat gacattttta ctagacctta tcaacggggt
     5221 gcataaattc cttggctatt tagacatcag tcctaaatat cttaaccgtg gatatacgat
     5281 tttaagtgtg attcccacgc tttatttatt gagaattgtt tatggacttt ggcaaaacca
     5341 aaactatctt caatttttct tatatggact tgcatttcta gtgcttgttt attttactgt
     5401 tttaaatgta ttttattact ttttagataa aaacacgaaa gccgatgtca ctcagctttt
     5461 tgtgaagtat ttacctgatg aagcgttcaa catccagaca gaagaatcga tagataatgg
     5521 aaattcgatc gataaagtga atacacaaga ggtcatggtt tcttatgacg aagattatca
     5581 attgaaatta gccgagaata tgcggtattt gattggaaat ggtgagatca aaacaaatga
     5641 actcggtcgc atggatggat ttctggtcga tagaaataca ttgtatcctt attactatgt
     5701 caagaaaaca ggagagaaag agtatgttct aaatattgga cgaagctact cggacatgga
     5761 aaaagtaggg accattcgac ttgactcaac agaagacaca ttggaacctg tcggattgtt
     5821 catcgtcggc ggtgattttg tcaaagacgg gttacgctat catgagcctt atcgtctgaa
     5881 attgattgca aaaaaggcac agtcgcagga agaaccagtg gtttatagtc gtagccaaag
     5941 aagaagacat tcataatgtg atgaaacgcc gtgtatcagt ggttgcgata aaaacgttta
     6001 ttcggaataa gggggatgtg acacgtcttt tgtcacgttc cttttttata taacgagaaa
     6061 gataagacga acacatcact tattctttcc tttcatttat aaacattgta gaataaagaa
     6121 agtacaacga agtattgtga aaaaacgtaa taaataacga ggaaaataca agtaggaggt
     6181 aagctgtgaa agaatttttg aaaaattggg ggattttgat ccttgtatta gcggctattt
     6241 tattagctag agtgtacgtg tttacaccag ttactgtaaa cgggcattcg atggacccta
     6301 cattaagtga tggacaacga ttgatttcat ctaagatctc aaactatgaa cgaatggata
     6361 ttattactac aaaggaaccg ggggatgaag aacggatgat tgttaaacgg atcatcggta
     6421 tgcctggaga tacagtcaag atggaaaatg accagttgac cattaatggc aaaaagtatg
     6481 atgaacctta tctggatgag ttcaaaaaag aattttcaga agataagtta caaggagaat
     6541 atgcttatag ctcaggattc caagcacaag cagaaagttc atccaccttt acgagtgact
     6601 ttgaatatac tgtgccaaaa ggaaagtatc ttgttctagg cgataaccgc ttgatttcaa
     6661 aagacagccg aatgtttggg ttagtcgaca aagatatgat ccaaggaaaa gttgttttcc
     6721 gttattggcc tctttcagaa attaaaatca tgtgatgaaa aaataaaaag aagctgtgac
     6781 atgttttttg tcacagcttc tttttccgaa aattccttct tatttcttgg agttgctcac
     6841 tttttttgca atctcatttt aagctttttg gctgatggat aagaaaagcg aagcgttttt
     6901 gttcataaat agcttttccc cacgatggaa ggtatgacta gtagcagaga caaagcccgt
     6961 tttttccaat cgattattca attcttcttg gctaaagcca ttatggactt tcggatgact
     7021 aatttgttca ttcttgtcaa agtctacgat gatcaattga ccgccaggag ctaaaatttc
     7081 ataaagtttt gtaagaatat tctccgtatc aggaatgtgt aaaagtacaa gtgatagaag
     7141 gatgagatta gcttggacag caggaaattc gacagaagca tcagcgtgga tcgttcggac
     7201 atttttcaga tctgctgctt ggatcttttc ttctgccatt ttcagcatag tttctgaagc
     7261 atcggcaatg atcagttctt tgaatctttc cgcaagcggt aaactaacaa gcccagttcc
     7321 gcctccgtaa tccagcagga ctttttggtg gctgtcgcgc ggtagataag tagttagttc
     7381 ttcgcgaata atcttggcta atgcttgacg gtcaggggaa tcataatggt tagcgatgga
     7441 atcaaaaata ttttttgtca tcaaatagct ccttacgtaa aactttgaaa ttttaactta
     7501 ttatagcacg tgaaaagaaa gaaagcatgc tatactcaat tcaaaaaggg agaatctaga
     7561 aagaatatgc cgatatcgtt tgtaaaagat agagaggaaa aaggaaagtg tgtccgagaa
     7621 atattactag acttgcctga gtggtttggg cttcctgaat caacggaaaa atatatcgag
     7681 gaatcaagta aactaccttt atggtgtgaa aaaagaaaag aagaatattt aggctttatc
     7741 actctttcac agacaagtga agatactgcc gaaatctatt ctattgtatg ggaataaaaa
     7801 aagactatca tcaccaagga ttaggaacaa aactttttaa agaagcagaa ggttacgcag
     7861 caaaacatta caaatatttg caagtgaaga ctgtagacga ggggcactac tctatatatg
     7921 atcaaactat ttgtttctac gaatcattag gattttcaag gttggaagtt tttccaaatt
     7981 tatgggatga atggaaccca tgtttagttc ttgtcaaaaa attggaacaa aagtgagcaa
     8041 tagatttaat actaatctgt gcttgatgaa aattctcact gtaaaaaatg ctgaaaatca
     8101 agtataatac cataggaaca aactggaagg tgaaaaagag catggcacaa ctatttttta
     8161 agtatggcgc aatgaatagc ggcaagacga tcgagatcct aaaagtcgca cataattatg
     8221 aagaacaaaa taagccagtt gtcttaatga caagcggtat tgatacaaga gatggtttag
     8281 gcgttgtttc aagccgaatc ggactgaaac gtaatgcgat gccgatattt gaagaaacag
     8341 acgtatttga cgtaattcag aaaatggaaa gcaaacccta ttgtgtgctg atcgatgaag
     8401 cgcaattctt aaaaaagaaa catgtcattg cttttacgag aattgtggac gaattggata
     8461 ttccggttat ggcatttggt ttgaaaaatg attttcgaaa cgagttattt gaaggatcaa
     8521 aatatttatt gctttatgca gacaaaatcg aagaaatgaa aacaatctgt tggttctgcc
     8581 acaaaaaagc aatcatgaat ttacattata ttgatggaaa ccctgtttat gaaggcgatc
     8641 aagtgcaaat tggcggaaat gaagcttatt accccgtttg tcgaaaacat tactttcacc
     8701 catcaatgat tacagaagga gaataagcat gtacgatcaa ttacaagcaa ttgaagaccg
     8761 ttatgaagag cttggagaac ttttaagtga cccagaagtg atttctgata caaagcgttt
     8821 tatgcagctt tcaaaagaag aagcaaacac gcgcgaaaca gtcgaagttt atcgtcgtta
     8881 taaagaagta gtacaaggaa tcaccgatac agaagaactg cttggtgaaa aactggatga
     8941 tgaaatggct gaactagcaa aagaagaatt atctgaatta aaaaaagaaa aagaagtctt
     9001 agaagaaaaa atcaagatct tgcttttacc aaaagatcca aatgacgata aaaatatcat
     9061 catggaaatc cgtggagcag caggcggaga cgaagcagca ttattcgctg gcgatctttt
     9121 caatatgtac caaaagtatg ctgaagcaca aggctggaaa acagaagtga tggaagctaa
     9181 cgttaccgga atcggtggat ataaagaagt gatcatgatg atcaccggag acaacgtata
     9241 ttcgaaatta aaatatgaaa gtggtgcgca ccgcgtccag cgtgttcctt caactgaatc
     9301 gcaagggcgt atccatactt ccaccgcaac agttgtcgtg atgccagaag ctgaagaagt
     9361 agagatcgat attgaagaca aagatatccg tacagatatc tatcatgctt caggtgctgg
     9421 cggacagcac gtcaataaaa cagcatctgc tgtacgtttg actcacttac caacagggat
     9481 cgttgttgct atgcaagatg aacgttctca aatcaagaac cgtgaaaaag caatgaaaat
     9541 cttgcgtgct cgtgtgtacg ataagatcca gcaagaagca caaagcgagt atgatgccaa
     9601 tcgaaaatca gcagtcggaa ctggggatcg ttcagaacgg atccggacgt ataatttccc
     9661 gcaaaatcgt gtgactgatc atcgtatcgg attaactatc caaaaattag atcagatttt
     9721 agcagggaaa ttagatgaga tcgttgatgc attgatcatg tatgatcaaa catcaaaact
     9781 ggaagctatg caaaatgggt aaaatgacct atcgagaagt cctgtcacgg gcttcttctt
     9841 ttttagagga tcatgggaaa gaagcatata gtatccaatt tttatttttg gaacgaaaac
     9901 aatggaaaaa acttgattgg ctccttcata tgaacgaaga gatttcagaa gaagaacagc
     9961 ggttgatcga aaccgatctg cgacttctgc ttgcagatca tccacctcag tatttgctag
    10021 gatatgcaga tttttatgat catcggttaa aggtaactga agcaacactg atccctcgac
    10081 cagaaacaga ggagttagtc gaatggtgtt tagacgagac gcctggtgta ccgttagaag
    10141 tgatagatat tgggacaggg actggagcaa ttgctattag tttgaaagca gcaagaaaaa
    10201 attggcatat atctgctgtg gatctttcag aagaagcatt agaagtagcg aaagaaaatg
    10261 ctcagaaaga aggaacgaaa atcagttttt atcatggaga tacattagga ccagtaatgg
    10321 atcagcaatt tgatgtgatt atttctaatc cgccgtacat cagtcggaat gaatgggaac
    10381 tgatggatga aagtgtccgg tcattcgaac ctaaaatggc cctttttgct gagaacgatg
    10441 gattagctat ttatgaaaaa atagcagttg aagcaccaag cgtactaaaa tcaaatggaa
    10501 agattttttt agaaatcgga tttcgccaag gtgaagcagt aaaaaaaata tttcagcaag
    10561 catttccaga taaaaaagtg gcgataaaaa aggatctttt tggcaatgaa cgaatgatcc
    10621 aagtttatgg ttgaagttgt ggatgaaaaa ttttttattt gtttttttat tataaaaaaa
    10681 cacttatttg acaaggatgt aaacgacttt ttggtttatc aacaagttta tccactattg
    10741 acaaatagat tatccacaaa cagtggaaaa cttgaggata aactcagaca aaataagaat
    10801 gtatgaaaaa ggggttgtgg ataaactgtg gaaacgaaac gatatgatga aacagaattg
    10861 caagaggcag ccgaagcact gaaagaagga gaacttgttg cttttcctac cgaaacagta
    10921 tacggattgg gcgccaacgc tcttttacca aacgctgtca agaaagtgtt ttctgtaaaa
    10981 gggagaccgc aagataatcc attaatcgtc catgtcgcct cttttgaaca agtaaaagag
    11041 tatgtggaca actttcatcc ggcagcagag aagattgtga agaacttctg gcctggacca
    11101 ttaactttga tatttaagat caaaaaagac actttgccct cagttgtgac gggaggatta
    11161 tccacagctg catttcgtat accggataac aaaaaaacac tagaagtaat cgaactttcc
    11221 ggtgttccgc ttgtcggtcc aagtgcaaat acgtcgggaa aaccaagtcc aacgactgca
    11281 gatcatgtat atcatgatct acaaggaaaa attaccggga ttattgatga tggagctacg
    11341 cgaattggtg tagaatccac tgttcttgat ttaagtgatc caacagccat gcctatgatt
    11401 ttacgaccag gtgcagtaac gaaagaacaa atcgaagcag tcatcgaatc tcctgttgct
    11461 attgatcagc atcttgtaaa agaaaatgaa acaccaaaag cccctgggat gaaatacaaa
    11521 cactactcac ccgatacacg tgttctgatg gtcagagatg gcgattggtc aactgcagtt
    11581 caatgggcaa aaaataagaa gatccgtgca ggtgtcatag caagccccga aattgcggat
    11641 caagtgcgga cagatactgc agctgtttat atgtataacg acaactcagt agaagcggcg
    11701 gctaaaggat tgtttgcagg tctaagagga ttagatgaac caacacttgg attagatttg
    11761 atttttgtac aagtgtatcc agaaactgga ttggggaatg cttacatgaa tcgcttgaaa
    11821 aaagcagcag gacaaaatta ttttgaaaaa tgatcaattg gttattgtcg cattctttta
    11881 gtatgataca atgctatcaa gtaaatcttc tatcagtgaa cgaaaaacta agcgtaacta
    11941 atcgtaatag aagagatacg aatatttggc aatttgtttt tgatgggaaa aataaaattg
    12001 taggaaaata aaaaggagct gttgtttgtg gtagattaca aaacgtttga ccctgatctt
    12061 tgggctgcga tcgccaaaga agaagaaaga caagaacata atttggagct gattgcctct
    12121 gaaaactttg tctccgaagc agtgatggct gcacaaggaa gtatcttgac aaataaatac
    12181 gcagaaggtt atccagggca tcgctattat ggtgggtgtg aatttgtcga tatcgttgaa
    12241 aatttagcga ttgaccgtgc aaaagaatta ttcggagcga aatttgcgaa cgtccagcct
    12301 cactccggct ctcaagccaa cacggctgct tatctcgctt tggtagaacc aggggacacg
    12361 atcttaggaa tggatctttc cgcaggcgga catctaacac atggatcacc agtcaacttc
    12421 agtggcaaaa cctatcactt tgttgcttac ggtgttgatc cgacaacaga agttattgat
    12481 tataatgtgg tacgtatttt agctagaaaa catcaaccaa aattgatcgt agcaggtgcc
    12541 agtgcttacg gacgaacgat cgactttgcg aaattcagag aaatcgcaga tgaagtaggc
    12601 gcaaaactga tggtagatat ggcacatatc gcaggattag tagcagcagg acttcatcct
    12661 aatccagttc cttatgcaga tatcacaacg actaccactc ataaaacatt gcgtggccct
    12721 cgaggcggca tgattttgac aaacgatgaa gcattagcaa aaaaaataaa tagtgcggta
    12781 tttcctggta ttcaaggcgg ccctctagag catgtgattg cgggtaaagc agtagcattc
    12841 aaagaagcat tagatcctgc atttaaagaa tatagtgaac aaatcatcgc caatgcaaaa
    12901 gcaatggtca aagttttcaa tcaagcaatt ggaaccagag tcatttcagg ggcgacagat
    12961 aatcatctga tgttgatcga tgttcgcgag ttaggtataa atggaaaaga agcagagagc
    13021 attttggaca gtgtgaatat cactgtaaat aaaaactcga ttccttttga aacactaagt
    13081 ccattcaaaa caagcggtat tcgaatcggc acgccagcaa tcactactcg cgggttcaaa
    13141 gaagaagatg ctgtaaaagt ggctgaacta gttgtcaaag cattacaagc aaaagatgat
    13201 aacgctcaac tagacgaagt caaaacaggt gtacgcgaat taactgaaaa atttccgttg
    13261 cataaaaaat aaaaatttaa attccattag taaacagaaa agttcaatgt tgggcttttc
    13321 tgtttatttt ttgggaaaac ggattctttg acaatatcgg tgatgctctc tagtatatga
    13381 ggtgtaattt tagtcttcac actaaatctc agggaggaaa agtcatgact gatcgtatct
    13441 cgttagaaaa ggaagcagta gcattcagtg atgcaaatgc gcctcatccg cgtatctatg
    13501 aactagcacc ggaagaagga agagttttac tagaagaagt acaagaatca cctgtgaaca
    13561 aatattccgt cgatattgaa gacttgatgg tggatactgg cgaatgggga aaactcaata
    13621 ttcgtctcgt acgaccagaa gggaatatgg ataaattacc agttattttt tatattcatg
    13681 gagcaggctg ggtttttggc agtgctcaaa cccatgataa attgatcaga gaacttgcgg
    13741 ttcgtacgaa ttccgtcgtt gtctttccag aatatagtcg atcaccagaa gcaaaatatc
    13801 caacagcaat tgaacaaaat tatgctgtgt tgcaacagct gaataaatta gccaagtcaa
    13861 aaaatctgga tctcgagaat ctaatcgttg caggagattc agtaggtggt aacatggcta
    13921 cagtcatgac gatcttaaca aaagaacgaa agggcctccc aatatccaag caattgctgt
    13981 attatccagt cacaaacgct gattttgata cagattccta tcatgaattc ggagaaaact
    14041 attttttgac aagagaagga atgatgtggt tttgggacca atatacgacg aatgaagaag
    14101 agcgtgctga aatcacagct tcgcctttga gagcaaatat aaatgaacta aatgatcttc
    14161 cttcagcaat gattttaacg ggagaagcgg atgtattaag agatgaaggt gaggcttatg
    14221 cgcgcaaact tcgtgatgca ggggttcctg ttacacaagt acgcttccaa ggaatcatcc
    14281 atgattttgt catggtaaat gctatggatc agacaaaggc aacgcgtgca gcaatggcat
    14341 tatctacttc ttggatcaag aaaaaagaat aagctttgct tgtccgcagg aaaacttctt
    14401 ttcaaaaacg gattttctta ggaaaaatgg cgagtttgac tagtcaatca cttgattttg
    14461 ttttacaatt aaaaagaaac gacagaacaa aggagagctt aaaaaatggg caaatttcaa
    14521 gtgatcgatc atccattgat tcaacacaaa ctaactatta ttcgggacaa aaactgcgga
    14581 acaaaggttt ttcgtgaagt agtcgatgaa atcgctatgt tgatggcata tgaagtatca
    14641 cgggatatgc cattggaaga catcgtgatc gaaacaccga tcactgaaac aacacaaaaa
    14701 acgttgtctg gtaaaaaagt agcgattatt ccaatcttgc gcgcaggtat tggaatggtt
    14761 gacggtatct tggaattgat tccagctgct aaagtgggac atatcggtct ttatcgagat
    14821 gaagaaacat tggaaccaca tgaatatttc gtcaaacttc cagaagacat tgatgcacgt
    14881 cagttgtttg tagtagatcc aatgttggca acaggcggat cagcaatcat ggcaatcgat
    14941 gcattgaaag aacgtggcgc gtcgaacatt aaatttgttt gtttggtagc agcacctgag
    15001 ggagtaaaag ctttgcaaga agcgcatcct gatattgaca tctatacagc ttctctagat
    15061 gaaaaattga atgaacatgg ttatatcgtt cctggactag gggatgcggg tgaccgtttg
    15121 tttggtacaa aataagtaaa tacgaaaaag ggtgtgacac aacttttgtc acatcctttt
    15181 ttggtcattt ttaacgtttg atgcagaaca gctttttcac agctttcgtt tgcatataaa
    15241 aaaaccatcc caaaaatgca gagatggtca aattcaaatc ttttttagtt ctttgtagtt
    15301 aacgattact acacccttac tttgcgtcat caagttcgca ttttctgcag tttcatcaat
    15361 ctcaacaaca gcggaatttt tcaattgctt tgaaatcgtt ccagtttgcg acttgccgtg
    15421 aattgtgaac attacgtgag tattgatctt gaatttttca ccaatttccg atggtacgat
    15481 agttgtatca aattttccaa aagttgccat tttcataaaa cctgcctttc acatcaatta
    15541 taccacgttt tatgaaaaat atcatgaaat taaactaatt ttccatcgga ctctttacaa
    15601 tcagccaaat agagtatata atttcttttg gataaggcca gcttagctca gttggtagag
    15661 caacgcactc gtaacgcgta ggtcgtaggt tcgatcccta tagctggcat taaaagagaa
    15721 gacagatata tcaaggattt tt
//
LOCUS       DAZKLM010000061        15314 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3764
            SAMEA115706913-rid22582563.denovo.063, whole genome shotgun
            sequence.
ACCESSION   DAZKLM010000061 DAZKLM010000000
VERSION     DAZKLM010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706913
            Sequence Read Archive: ERR13196647
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15314)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15314)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287823.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287823.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,004
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,849
            CDSs (with protein)               :: 2,849
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 41 of 96
            Pseudo Genes (incomplete)         :: 52 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 15 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15314
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706913-rid22582563.denovo.063"
                     /isolate="VRE3764"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            278..787
                     /locus_tag="ACG842_001864"
     CDS             278..787
                     /locus_tag="ACG842_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFQ0248398.1"
                     /translation="MVQGIERGVYVVDKIQFNQVIEEFIKVNNLMTHIQKRPIRITND
                     LKLSTSMIHLIDVIGNYPETSITELADRLGVTKGAVSQQIPTLEKMNLISISQKKGNK
                     KTKLVSLTKYGRKVYESHNYLHEELYSSINEALESFSPDQISTIKQILEKVSYTINEY
                     QVSLTRKED"
     gene            880..2042
                     /locus_tag="ACG842_001865"
     CDS             join(880..1156,1156..2042)
                     /locus_tag="ACG842_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFQ0248399.1"
                     /translation="MSRRQRRTYSKEFKQQVVDLYLAGKPRAEIIRDYELTTSAFDKW
                     VKQAQTTGSFQEKDNLTPEQVELIALRKKNKQLEMENDILKQAALILGPKRQVIDTNK
                     HKYSISAMCKVLNISCQTYYYQAKPVEDESELEELVQEEFIRNRKAYGTRKLKQCLAN
                     LGLQVSQRRIGRMMKQRGLRSTYTLAHFKFHSSTCNEAKTANILDGTFLQEKSLEAIV
                     TDLTYVRVGKKWQYICLILDLFNREIIGYSCGEKKDATLVKEAFAKIPYSLNNVQLFH
                     TDRGKEFDNQTIDEILWAFNITRSLSKKGCPYDNAVAESTYKSVKVEFVHQYRFETLA
                     NLRLELFDYVHWWNTIRLHGTLGYETPISFRQQRLAQRILDNELGCDTGGEAP"
     gene            2322..3302
                     /locus_tag="ACG842_001866"
     CDS             2322..3302
                     /locus_tag="ACG842_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFQ0248400.1"
                     /translation="MKKIGIREVAKEANVSPTTVSRVLNDRGYLSEETKRKVFDAMKK
                     LEYYPNELARALFKNETYTVGLIFPTITNPFHAELIQDIEFELSSRGYKVLLCNSLNN
                     PTKEKEYLTMLRKNQVDGIIVGTHNKDIADYDIPGLPIVAIDRSLGENTVTVSCDNYR
                     GGELATQLLVNHGCQNILCIRGDSKIKLPANSRTLAYKDVLNKFMLEPLVLEVPFVKE
                     INEKNEIIKTYLETHPNIDGVFAGDDLLASLAINNLGSSGKRVPKEVKVIGFDGAQQT
                     LIYNPELSTIQQPIDQISKVAVKKLLNMIKGEKETDELSLPVKLIKKNTA"
     gene            3424..4842
                     /locus_tag="ACG842_001867"
     CDS             3424..4842
                     /locus_tag="ACG842_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HFQ0248401.1"
                     /translation="MEKLLIKSNNHQLYYQPKDVWVGDVMPYGKEGKFFIYHQRDTRN
                     PVPFGEPFGWALATTKDFVHFEDFGESLLRGDDNERDQFIYAGSVFEANGKVHAFYTG
                     YNREFLAQGKTSQVLLHAESDDFVHWEKSETALELEPQEGYDIRNWRDPFVVWDDEKE
                     EYLLILGARKGQDKHLQTGRLVEFTSKDLTNWEFKGDFWAPGLYTMFEMPELFKIGDW
                     WYLVYSEYSDGNKILYRMSQSLEGPWIKPKDEAFDGRAYYAGRTAYDGKRRVLFGWVP
                     TKDQENDKNNYLWGGTLVPHEVYQKEDFSLGVKPIDEVWNAFINLHDIADVEIETADT
                     REEVTLVKNSGTLFNLETTISFDENLSDFSLRLYKNLETDESYEYRFNLDEGRLTFDK
                     NPCYPWFQYMDKGLIRPISLKPNQSYKLKVIVDDNIMTLYIDGIALNARVHGCFGQAI
                     TITATNGSMTAINTKFSNEIKK"
     gene            4913..6382
                     /locus_tag="ACG842_001868"
     CDS             4913..6382
                     /locus_tag="ACG842_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFQ0248402.1"
                     /translation="MDYKKIAEDVLENVGGKENISSATHCITRLRLILKDKSKANMEM
                     LEEIEGAKGVLFNSGQLQVIFGTGIIEKVYKEFVELTGAKEVSVSEMKDEGVNNSGNR
                     LQRAFKVFSDIFIPIIPAFIGAAMVLGIRSLITTPGMFGMDGSFADHNVFLNDLSKFL
                     GIIATTFDYLPVLVMYSATKRFGGNPILGLLVGFVMIHPGLANRNDFVMGTIKPDYWH
                     LFGLSIPAVAFQGGVFPAILTSWFLAKVEKITNKYSPQVLAYILVPTVTIVLANFALF
                     LVFGPLGNFIGNVLGGIIDFLYMKMGAFGAFVFAGALQPLVVTGTQHAIQGIEANLVA
                     TTGFNYIQPIWSVSIIAQGGGCIGMYLLFKKKSKDRDIAISSFIPTLVGITEPAIFAV
                     NLKYSIVPFVCSFIGAGFGGAFMKIFDVKAIGQGLTVLPGLTIANPVLPYIFGNLIAF
                     ILPIIFILAYDKYKVFIPEAKHRKFAEKTDSKKLPVQVK"
     gene            6392..6859
                     /locus_tag="ACG842_001869"
     CDS             6392..6859
                     /locus_tag="ACG842_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HFQ0248403.1"
                     /translation="MLNFFRKNEISVVAPATGVFEKLEKLSDPVFSKGMMGQGFAIDA
                     KNETIVSPIDGFVSSIFPTKHALGIKTKSGIEILVHVGIDTVELNGEGFDIKVQEGQK
                     VKAGDILMTINFVVFEKNHKTKDVIVVFPDYQDEIFIPKLEEVKQGQELFVLG"
     gene            6895..7767
                     /gene="scrK"
                     /locus_tag="ACG842_001870"
     CDS             6895..7767
                     /gene="scrK"
                     /locus_tag="ACG842_001870"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008865 - fructokinase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase ScrK"
                     /protein_id="HFQ0248404.1"
                     /translation="MVFGAIEAGGTKFVCAIGDKDLNIFEKVSFPTTTPKETMNLVLD
                     FFNHHKDDLEGIGVGSFGPIDIHCESKTYGFITSTPKLAWQDFDFIGSLKKQLNIPTA
                     WTTDVNASCYGEYVSGNGRGLSSVVYYTVGTGIGGGAIQNGNFIEGFSHPEMGHMLVK
                     RHNKDNFSGTCPFHGDCLEGLAAGPAIERRSGKKGQEVTENDVTWEIEAYYIAQCAYN
                     TTLMLSPDVIIFGGGVMKQKHLKKKVQDKFIELINGYVAIPDVDNYIMTPKLLDNSGI
                     VGCLALARDVKEKS"
     gene            7849..10383
                     /locus_tag="ACG842_001871"
     CDS             7849..10383
                     /locus_tag="ACG842_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFQ0248405.1"
                     /translation="MKKKMKDEVLEYIKNNLKYYDFSAQDIAMKFCIKRNVASHYLNQ
                     LFSDGKLLKNDSVRPVMFKYNQQKPKDCFSKFIGADISLKSTIDKCKATVMYPPNGLP
                     LIIKGNSGVGKSFLASLIYQYALDRKVIHNDAKFVVVNCADYANNPELLSAVLFGYKK
                     GAFTGAEKDTAGMLSSANGGYLFLDEVHNLSAENQEKLFLLMDSQKYRKLGESVQWEY
                     ANVRLILATTEDKNTSLLATFRRRIPAEITLPDYNNRSTSEKIQLLFEFLKDEAITID
                     SKIFCSIELFTDLLSKTFEGNVGELRNEIKLLCAEGYLGNKRKSSIYLGDKLDSGFWI
                     DPEISIDLKKMFLKSLSEIDLIELFQNHISIEESMSQLRDRILKECAGSSEYNYLESD
                     EFIALRNYVVNKISPIIDSTGLCLLDEFLADISLFIMFIDVIEETSRNFLLSKFRKFR
                     MKNDKQKLLANEIWSSLEVEESKQELLLKWILFLVKKFYVKIPETHCLIVMHGKITAS
                     AIARETNKLLNTYVYEAFDMPIEGETSDLINLINNFCKSIDTTNGLILLVDMGSLEQM
                     YEKIESNVIGDLVILNNVSTALAIECGIQVCQKKPISHFYQMDFDSFQVKVQYYKGLS
                     QKKNVIVSCLSGEGISEKVKDILKRYLDNQVEILIFDFNALKKIAKEKDTMIFKNTIC
                     VLSTTEFYIQGIDCLNLENLINGNQTLEKLNKYMDSDSCEFCLNELVKLFTIEGASTK
                     LRFLDSKKVFNEIEKVLYLYEKYYDVKIPSFLRINLFLHLSGMIERIMIGDGITNEHL
                     KEGAQLFDTFLNVSKDFFSEIKDIYCIEIPKGEYELIYTIFEQTIF"
     gene            10539..11312
                     /locus_tag="ACG842_001872"
     CDS             10539..11312
                     /locus_tag="ACG842_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFQ0248406.1"
                     /translation="MAFWQTFLVVLYGFFINWDKEGPKIGTSQPVVAGFLTGLIMGDI
                     QTGLYIGGTLQLMTLGITSFGGASVPDYQTAALIGTYLAISTNQDASIGITLAIPVAM
                     LIVQLDVLKWSTNIFFQQKAEKFAQKEDYQKLEWMQYLASINTSFVSGIPVLLTVILG
                     PTLVGSAINYIPEWVTGGLKVAGGLLPAVGIGMLLKYLPTKDYFAYLLVGFVLAVYFQ
                     IPILGISLLGAAIALIVYKNRSNNTQVAPAATGGFDEDE"
     gene            11305..12096
                     /locus_tag="ACG842_001873"
     CDS             11305..12096
                     /locus_tag="ACG842_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFQ0248407.1"
                     /translation="MNKKLTKIDKQVIFRWILNGATSLNYEKMEGLGYAYSMLPFIKD
                     TYKDDPEGMKQSVLAHLQFFNTTPYTAPYIIGMNVGIEKEEKNKSLEAVSALKTGLMG
                     PLAGIGDSVFVVIPWTIFGAIAANMALEGSFVGIILWILASILLRITAYPLYLAGKES
                     GASLLTQIETKLKSITDAVSILGLMVVGALIATVVKVNVGFEFKQGDLTMTGNEIFDQ
                     VMPGLLPACVVAIVYYLLGRKVKPVYVILLILVVSIALRALNILK"
     gene            12106..12597
                     /locus_tag="ACG842_001874"
     CDS             12106..12597
                     /locus_tag="ACG842_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HFQ0248408.1"
                     /translation="MDEKMKGIIHVRIDDRMIHGQVATQWTNQLSANRIMVINNEVAN
                     DDVKKAVVRLAAPPNVRTSIITREVAVKNILSGKYEGQKVLIVAVSPIDVKFLIDNGL
                     PITSVNVGNLSRRNGTERIRPTINITEEEREAFKELISDGVEVTVIQTPRDSKEFLKD
                     FIY"
     gene            12615..13025
                     /locus_tag="ACG842_001875"
     CDS             12615..13025
                     /locus_tag="ACG842_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFQ0248409.1"
                     /translation="MEFIITSHGNIAIGFVDTLKMFFGDNLNCHVVTLGDAGIEEFRE
                     NIEKIVSQVKNQEVMVFADLIGGTPFNEFAKRSEVFQNGFQLLGGMNLGILIEAVNLR
                     LQGKKSDDIIENLRNMNTIACLADMQRHTNEEDE"
     gene            13029..14429
                     /locus_tag="ACG842_001876"
     CDS             13029..14429
                     /locus_tag="ACG842_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-fructofuranosidase"
                     /protein_id="HFQ0248410.1"
                     /translation="MQKIFYKPKDFWFGDCMPFYKDGTFYLFHQRDTRNPKPLTDPFG
                     WSLVTTTDFVKFEEYGEVIHRGGNDDIDQYIYAGSIVQGINEYLALYTGHNRKAKLDG
                     KTSEVLMIASSKDLIHWKKDGEAAKLVPQEGYDKNDWRDPFAIFDQDHGKYVLILGAR
                     KPASKKKPTGRLVYFESEDLKDWNFKGDYWANNEFNMIEMPDIFEIDGTWYLLFSEYC
                     EDKKTKYRVGAGLFSDWKSPVDEAFDGKAYYAARTVGTNETGRFLTGWVATKEGENDQ
                     NNWDWGGALVPHQIIQKTDKTLGVKLPEKIIENFKEITPFSSFKLEKEFGKKERVIVP
                     KIEDYFLFETTLTITSLTKEFALKLFVDSDTQEGYEFRISLINNQLTFGKTPNYPWPQ
                     MFDKGLQRPLFLEIGKEYELKLIVDDTIAVLYIDNVALSTRMYDKVGTALSMTVTEGA
                     IKVDKLDYSINYECGF"
     gene            14452..14826
                     /locus_tag="ACG842_001877"
     CDS             14452..14826
                     /locus_tag="ACG842_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFQ0248411.1"
                     /translation="MKLNGLQHIGIPTKDFGASKIFYESLGFSLINEEMNVGSKVGFF
                     ELNGTIIEIWEDKTTECRGAIDHLALDTDDIDEVFNSIQDLGYELLDKNIMELPFWEN
                     GIRYFNFYGPNHEIIEISQMKK"
BASE COUNT         5315 a         2156 c         3060 g         4783 t
ORIGIN      
        1 ttttttattt gtctcgctta attatacaaa cggcgtgata aaaaacgaga aatgaattat
       61 aggtttagac ctttatcatt tctcgtttta ttttattgta tcaaggctct atcataagtg
      121 gtaaaatcta cttttaaatc acatgtaatc attgataagt ccagtgtttt agcggataat
      181 cagcttttta tttatttttt cttataaatt tgataaatag aaattagtat agccctaaac
      241 aaattatttg tgttttattg tttagggcta tactatattg gtacaaggaa tagaaagagg
      301 tgtttatgtg gttgataaaa ttcagtttaa tcaagttata gaagaattca tcaaagtaaa
      361 taatttaatg acccatattc aaaaacgacc aatcagaata actaatgatc taaaactcag
      421 tacgagtatg attcatttga ttgatgtgat tggaaattat cctgaaacta gtattacgga
      481 attagctgat cgattaggcg ttaccaaagg agctgtatcc caacagattc ccactttaga
      541 gaaaatgaat cttataagca tttctcaaaa aaaaggaaat aagaaaacta aacttgtatc
      601 gttaacaaaa tatggtcgaa aggtgtacga atcacataat tatttacatg aagagcttta
      661 tagctcaata aatgaagcgt tagaatcatt ctcaccagac caaatttcaa ccattaaaca
      721 gattctagaa aaagtatctt atacaatcaa tgaatatcaa gtaagtctga caagaaagga
      781 agattgacat gaaaaaaatg atttttgaat cggctacact agactagaca gaaaaaataa
      841 ggtgtgtaaa ataaatgaaa acacactgga ggaattttta tgtcaagacg tcaacgtaga
      901 acctattcaa aagaattcaa acaacaagtc gtcgatcttt atctcgcagg aaagccccga
      961 gctgaaatca ttcgagatta tgagctgact acctcggcct ttgataagtg ggtcaagcaa
     1021 gcgcaaacta ctggctcgtt tcaggaaaaa gataacttga cccctgaaca agtagaacta
     1081 attgcattac gaaagaaaaa taaacagctt gaaatggaga atgacatttt aaagcaagcg
     1141 gcgctgatat tgggacgaaa agacaagtaa ttgataccaa taagcacaaa tactcaatat
     1201 cagcgatgtg taaagtgtta aacatctctt gccaaaccta ttattatcaa gccaaaccag
     1261 ttgaagatga atcagaactg gaagaactcg ttcaagaaga atttattcgc aatcgaaaag
     1321 cttatggtac caggaagctg aagcagtgct tggccaatct aggtcttcaa gtgagtcaac
     1381 gcagaatcgg ccgtatgatg aaacagcgtg gcttgcgttc aacatacaca ttggctcatt
     1441 tcaaattcca ttcttctact tgtaacgaag ccaagaccgc caatattcta gacggaacct
     1501 tcctacagga aaagtcatta gaagcgattg taaccgacct tacctatgtt cgtgttggga
     1561 agaaatggca gtatatctgc ctgattcttg atttgtttaa tcgagaaatc atcggctatt
     1621 catgcgggga gaagaaagat gcgacgttag tcaaggaagc ttttgcaaag attccgtact
     1681 cgttgaataa tgtacaactt tttcacacgg atcgtggaaa agaattcgat aaccagacga
     1741 ttgacgaaat tttatgggcc tttaatatca cgcgttcctt aagcaaaaaa ggctgcccct
     1801 atgataacgc cgttgcagaa tccacctata aatctgtcaa agtagaattc gttcatcaat
     1861 accgctttga gacattggct aatttgcgat tagagctatt tgattacgtc cattggtgga
     1921 ataccatccg gctgcacggc acgttaggct atgagacacc gatcagtttt cgacagcaga
     1981 gattggcgca gcgaatcctt gataatgagc tcggatgtga taccggtggc gaggcacctt
     2041 aattatttga atgagcttct gccttagaga atcagattcg aggaggctcg ttgtcaaggg
     2101 cagtcggaac accagcagca agcttacgca ataccttata atttttgtca aaaaaagtgt
     2161 tgccattcca catatatctt taaacagcta ttgccagata aaaataaatg atattatata
     2221 tataaatact tatccgaaaa ggggaatgag agagtcaagg ttcttggaat gtcgaatagt
     2281 cgagtaggta gattttctat ctacaacctt gttgtttaaa aatgaaaaaa attggaatta
     2341 gagaagttgc gaaagaggca aatgtttctc ctacgactgt gtcgagggta ttgaatgatc
     2401 gtggatattt gagtgaggaa acaaaaagaa aagtttttga tgccatgaaa aaattagaat
     2461 actatcctaa tgaactggcg agagcacttt ttaaaaacga aacatatacc gtaggtttga
     2521 tttttcctac aattactaat ccatttcacg ctgagttaat tcaagatatc gagttcgagc
     2581 tttccagcag gggatataaa gtgttgctgt gcaatagctt aaacaatcca acaaaagaaa
     2641 aagagtactt gacgatgttg agaaagaatc aggttgatgg aattatagta ggaacgcaca
     2701 ataaagatat tgcggattat gatattccag ggctacctat tgtagcgatt gatagaagtc
     2761 ttggtgaaaa tacagttaca gtatcttgtg ataattacag gggtggggaa ttagctactc
     2821 aactacttgt aaatcatggc tgtcagaata tactgtgtat tcggggagat agtaagataa
     2881 agctacccgc gaatagtagg actttagcat ataaagatgt tttgaataaa ttcatgctgg
     2941 aaccgttagt cctagaagta ccatttgtaa aagaaatcaa tgaaaaaaat gaaataatta
     3001 agacatatct ggaaactcat ccaaatattg acggggtttt tgcaggagat gatttattgg
     3061 cttctttggc gattaacaac ttaggaagtt cgggcaaaag agttccaaaa gaggttaaag
     3121 tgattggatt tgatggagct caacaaacat tgatttataa tccagaatta tcaactattc
     3181 aacagcctat agatcaaata tctaaagtag cagtaaaaaa attattgaat atgattaagg
     3241 gcgaaaagga gacggatgaa ctttcacttc cagtaaagct cataaaaaaa aatacagcat
     3301 aagttaaatc ggcgaaaaat tccttcgtcg atttaaaaat atttttatga aacgggttga
     3361 catatgaaac gggttgacat atattagttg tgaagataaa aaccttttca ctggaggaag
     3421 aaaatggaga aattattaat taaaagcaat aatcatcaac tttactacca acctaaggat
     3481 gtttgggtcg gagatgttat gccttacggg aaagaaggaa aattctttat ttatcatcaa
     3541 cgtgatacta gaaatccagt tccttttgga gaaccgtttg gctgggcttt agcaactacg
     3601 aaagattttg ttcattttga agactttggg gaatctcttt taagagggga tgacaatgag
     3661 agagaccagt ttatctacgc tggttctgta tttgaggcaa atggaaaggt tcacgcattt
     3721 tatacagggt ataatcgtga attcttagcg cagggaaaga catctcaagt gttgcttcat
     3781 gctgagagtg atgattttgt tcattgggaa aaatcagaaa cagcattgga gttagaacca
     3841 caagaagggt atgatattcg aaactggaga gatccatttg tcgtttggga tgatgaaaaa
     3901 gaagaatatt tgctaatttt aggggcgcga aagggtcagg ataaacatct acaaaccgga
     3961 cgtttagtgg aatttacatc aaaagattta actaattggg aattcaaagg tgatttttgg
     4021 gctccgggat tgtataccat gtttgaaatg ccagaacttt tcaagattgg tgactggtgg
     4081 tatcttgtct attcagaata ctcagatgga aataaaatac tctatcgaat gagtcaaagt
     4141 ttggaaggtc cttggattaa gcctaaagat gaagcttttg atggacgggc ttattatgca
     4201 ggtagaacag cttatgatgg aaaacgtcga gtcctatttg gatgggtacc aacaaaagat
     4261 caagaaaatg ataaaaacaa ttatttatgg ggaggtactt tggttcctca tgaagtctat
     4321 caaaaagagg atttttcact tggggtaaaa cccatagacg aagtgtggaa tgcattcata
     4381 aatttacatg atattgccga tgtagagata gaaacagcag acacaagaga agaagtaacg
     4441 cttgtcaaaa atagtggaac tttgtttaat ttagaaacaa caatttcatt tgatgagaat
     4501 cttagtgact tttcactgag attatataaa aaccttgaga cagatgaatc ttacgaatat
     4561 cgcttcaatt tagatgaggg gcgtcttaca tttgataaaa acccttgtta cccttggttt
     4621 caatatatgg ataaaggact tattagacct atatctttaa agcctaacca atcttataaa
     4681 ctaaaagtga ttgttgatga caatataatg acactttata ttgatggaat cgctcttaac
     4741 gctcgtgtac atggttgttt tggtcaagca attactatta cagcaacaaa tggatcaatg
     4801 actgcaataa ataccaagtt ttcaaatgaa ataaagaaat aataaacgtc aatcatgtgg
     4861 caggtctgaa aatcaaacat gacctgtcac atttaaaagg gaggataaat gaatggacta
     4921 caaaaaaatt gccgaagatg tattggaaaa cgttggagga aaagagaata tctcaagcgc
     4981 tacacactgc attactcgtt tacgtttaat tcttaaagac aaaagcaaag cgaatatgga
     5041 aatgcttgag gaaatagaag gtgcaaaagg tgttcttttc aatagtggac agttacaagt
     5101 aattttcgga acaggtataa ttgaaaaggt ttacaaagag ttcgttgaat tgacaggagc
     5161 taaggaagta tccgtttctg aaatgaaaga cgaaggagtc aataattcag gaaatagact
     5221 tcaacgagct tttaaagttt tctcggatat ctttattccg atcattccag cttttattgg
     5281 ggcagcaatg gttttaggaa ttcgttcgtt gattaccact cctggaatgt ttggaatgga
     5341 tggctcattc gctgatcaca atgtattcct aaatgattta tccaaatttc tgggtattat
     5401 tgcaaccaca tttgattatt taccagtttt agttatgtat tctgcaacaa aacgttttgg
     5461 cggaaaccct attttaggat tacttgtagg atttgttatg attcatccag gactagccaa
     5521 tcggaatgat ttcgtcatgg gaactataaa gcctgattat tggcatttat ttggattaag
     5581 cattccagca gttgcgtttc aagggggtgt ttttccagca attttgactt cttggttttt
     5641 agcaaaagtt gagaaaatta caaataaata ttctcctcaa gtacttgctt acattttagt
     5701 cccaactgtg acaattgttc ttgccaactt tgcactattt ttagtgtttg gtccattggg
     5761 gaattttatc ggtaatgttt taggtggaat cattgatttc ctatatatga agatgggtgc
     5821 ctttggtgct tttgtttttg caggcgcgtt acaaccatta gttgtaacag gaacacaaca
     5881 tgctattcaa ggtattgagg caaacttagt cgcaacaact ggattcaatt atatccaacc
     5941 tatttggtca gtttcaatta ttgcccaagg tggcggttgt attggtatgt atttactctt
     6001 taagaaaaaa tctaaagata gagatattgc gatttcaagc tttattccaa cactggtagg
     6061 tattactgaa ccggctatct ttgctgttaa tttgaaatat tcaatcgttc cttttgtttg
     6121 ctcgtttatt ggtgctggtt ttggtggagc atttatgaaa atttttgatg taaaagccat
     6181 tggacaagga ttgactgtat tgccaggtct gactattgct aatccagttc ttccttatat
     6241 atttggtaat ctcatagcat tcattttacc aatcattttt attctcgctt atgataaata
     6301 taaagtgttt atccctgaag ctaaacaccg taaatttgca gaaaaaactg actcaaaaaa
     6361 attgccagtt caagtaaagt gagatgagag catgttaaat ttttttagaa aaaatgaaat
     6421 aagcgttgta gcacctgcaa ccggtgtttt tgaaaaactg gagaaattaa gtgatccagt
     6481 cttttcaaaa ggaatgatgg gacaaggatt tgcaattgat gcaaaaaacg aaacgattgt
     6541 atctccaatc gatggttttg tttcaagtat ttttcctaca aaacatgcct taggaataaa
     6601 aacaaaatca ggaattgaga tacttgttca tgtaggaata gatacagttg agctaaatgg
     6661 tgagggattt gatattaaag ttcaagaagg acaaaaagtt aaggctgggg atattcttat
     6721 gaccattaat tttgtagttt ttgaaaagaa tcacaagact aaagatgtca ttgttgtttt
     6781 tccagactac caagacgaaa ttttcattcc gaaacttgaa gaagtaaaac aaggacaaga
     6841 attgtttgtt cttggctaat gataagtaat taataataga atcgaggaaa atgtatggtt
     6901 tttggggcta ttgaagctgg tggaacaaaa tttgtttgtg ccattggaga taaagatttg
     6961 aacatatttg aaaaagtcag ctttcccacg actacaccca aagaaacaat gaatttagtt
     7021 ttagattttt ttaatcatca taaagatgat ttagaaggta tcggagtggg atcgttcggt
     7081 cctattgata ttcattgtga atctaaaacg tatggcttca ttacatcaac gccaaaactt
     7141 gcttggcagg attttgattt tattggaagt ttaaaaaagc aacttaatat tccaactgct
     7201 tggacgactg atgtaaatgc ctcttgctat ggtgaatatg tttctggaaa tggcagaggc
     7261 ctttcaagtg ttgtttacta taccgtagga acgggcatag gaggtggcgc tatccaaaac
     7321 ggaaacttca ttgaaggttt tagtcatcca gaaatgggac atatgcttgt aaaacgacac
     7381 aataaagaca acttttctgg gacatgtcca ttccacggag actgtttaga gggcttagct
     7441 gctggtccgg caatcgaaag aagatcaggg aaaaagggac aagaagtgac tgaaaatgac
     7501 gttacttggg aaatagaagc ttattacatt gctcagtgtg catataatac aacattgatg
     7561 ttatcacctg atgtcattat atttggtggc ggagtaatga aacaaaaaca cttaaaaaag
     7621 aaagtacaag ataagtttat tgagttgatc aacggatatg tcgctattcc cgatgttgac
     7681 aattatatta tgacaccaaa attgttagat aattctggta ttgtaggttg tttagcttta
     7741 gcaagagacg ttaaagaaaa atcttaattt ggaaaaaaga tcgtctcgac tatttgtttg
     7801 gttgatgcga tctttttttt caattagtaa ttgaagggac gcctaaaagt gaaaaaaaaa
     7861 atgaaagatg aagtattaga gtatatcaaa aataatttaa agtattacga tttttctgct
     7921 caagatattg caatgaaatt ttgtattaaa agaaatgtcg cttcccacta tttgaatcag
     7981 ttattttcag atggaaaatt actaaagaat gattctgtta gaccagtaat gttcaagtat
     8041 aatcaacaaa agccaaaaga ctgcttttct aagtttattg gagcagacat atctttgaaa
     8101 tccactatag ataaatgcaa agctactgta atgtatcccc ctaatggcct cccattaata
     8161 ataaaaggaa acagtggcgt tggaaaaagt tttttagcaa gtttgatcta tcagtatgct
     8221 cttgatagaa aagtaattca taatgacgca aaatttgttg ttgtgaattg tgcagattat
     8281 gcaaataatc cagagctatt gtcagcagtt ttatttggat ataaaaaagg agcctttact
     8341 ggggctgaaa aagatactgc agggatgcta tcttctgcta atggaggata ccttttttta
     8401 gatgaggttc acaatctaag tgcagaaaac caagagaaat tgtttcttct tatggattct
     8461 caaaagtatc gtaaacttgg tgaaagtgtg caatgggagt atgcaaatgt tcgccttatc
     8521 ttagccacaa cagaagataa aaatacctct ttgttagcaa cttttagaag acgcatccca
     8581 gctgaaatca ctctgccaga ttacaataat agaagtacat ctgaaaaaat tcaattatta
     8641 tttgaatttc ttaaagatga agcaattaca attgattcaa aaatattttg ttcgattgaa
     8701 ctttttactg atcttttatc gaaaacattt gaaggaaatg ttggagaatt gaggaatgaa
     8761 atcaagcttc tttgtgcaga aggttattta ggaaataaga gaaagtcttc aatttattta
     8821 ggggacaaac tagacagcgg tttttggata gatccagaaa ttagtataga tttaaaaaaa
     8881 atgtttctta aatcactttc tgaaattgat ttgattgaac tttttcaaaa tcacatttca
     8941 attgaagaaa gcatgagtca attaagagat agaatactta aagagtgtgc aggttcttct
     9001 gagtataatt atttagaaag tgacgaattt atagcattaa gaaactatgt tgttaataaa
     9061 atatcaccaa ttattgattc gacaggtcta tgtttattag atgaattctt ggcggatatc
     9121 agtctattta taatgtttat tgatgtaatt gaagaaacat caagaaattt tttgctgtct
     9181 aagttcagaa aatttcgtat gaaaaatgat aaacaaaaat tattggcaaa tgagatttgg
     9241 agtagtttag aagtcgaaga aagtaaacaa gaacttttgt tgaaatggat actttttctt
     9301 gtaaagaagt tctatgtgaa aataccagaa acccattgtc tgatagttat gcacggtaaa
     9361 ataacagctt ctgcgatcgc aagggaaaca aataagttgc tgaatacata tgtttatgag
     9421 gctttcgata tgccaattga aggtgagact agtgacttaa ttaacctaat caacaatttt
     9481 tgtaaaagta tagatacgac gaatggactc attctgctag ttgatatggg atcattggaa
     9541 caaatgtatg aaaaaattga atccaatgta ataggtgatt tggtaatttt aaacaatgtt
     9601 tctacagctt tagcgattga atgtgggatt caagtttgcc aaaaaaagcc aattagtcat
     9661 ttttaccaga tggattttga ttcgtttcaa gttaaagttc agtattataa aggattatca
     9721 caaaagaaaa atgtaatagt atcttgtctc tctggtgagg ggatttctga aaaagttaag
     9781 gatattttaa aacggtatct cgataatcaa gtggagattt tgatttttga ttttaacgca
     9841 ttaaagaaaa tagcaaagga aaaagatact atgattttta agaatacaat ttgtgttctt
     9901 tcaaccactg aattttacat tcaaggaatc gattgtttaa atttagaaaa tctaattaat
     9961 gggaatcaaa ccttagaaaa attaaataag tatatggact cggatagctg tgagttctgc
    10021 ttaaatgaat tagttaaact ctttactatt gaaggcgcta gtactaagct tcgcttttta
    10081 gattccaaaa aagtattcaa tgagatagaa aaagtactat atttgtatga aaaatattac
    10141 gatgtcaaaa tcccaagttt tctgagaatt aatctatttc tacatctttc tggaatgatt
    10201 gaacgaatta tgattggtga tggaataaca aatgaacatt tgaaagaggg agcgcaacta
    10261 tttgatactt ttttaaatgt atcaaaagat tttttttcag aaatcaaaga tatttactgt
    10321 attgaaatac ccaaaggaga gtatgagtta atctatacaa tttttgagca gactattttc
    10381 taaatagcat actagcgtct aaaaaaatga aagggtgtcc aaagtatatt tagacgctca
    10441 tttaattaat atttactgta ttttcccgct ttattggata atgttagttg gcacagtaat
    10501 tgcttatata aaagtataag aagaaaggag agaacaaaat ggcgttttgg caaacatttt
    10561 tagttgtgtt gtatggattt ttcattaact gggataagga agggcccaaa atcggaacct
    10621 cccaacctgt cgttgcaggt ttcttgaccg gacttattat gggagatatt caaactggac
    10681 tttatatcgg tgggactttg caattaatga cactaggtat tactagtttt ggtggtgcat
    10741 cagtaccgga ttatcaaaca gcagcattga tcggtactta tttagcaatc tcaactaacc
    10801 aggatgcttc tataggaata actttagcaa taccggtagc aatgttaatt gtacaactag
    10861 atgttctaaa gtggtcaaca aacatttttt ttcaacaaaa ggcagaaaaa ttcgcacaaa
    10921 aagaagatta tcaaaaatta gagtggatgc aatatttggc atcaattaat acaagctttg
    10981 tatctggaat tcctgttttg ttaactgtga tattaggccc aacgttagtg ggaagtgcaa
    11041 tcaattatat tcctgaatgg gttaccggtg gtttaaaggt agcaggggga ctattacctg
    11101 ctgttgggat aggaatgcta cttaaatatt tacctacaaa agattacttt gcctatttgt
    11161 tagttggttt cgttttggca gtttatttcc aaataccaat tttagggatt tcgttacttg
    11221 gcgctgcaat cgcactaatc gtatataaaa atagatcaaa taatactcaa gtagcaccag
    11281 ctgcaacagg aggttttgac gaagatgaat aagaaactga caaaaatcga caaacaagta
    11341 attttccggt ggattctcaa tggtgcaact tcattaaatt atgaaaaaat ggaagggttg
    11401 ggttatgcgt attctatgct tccttttatc aaggacacat ataaagatga tcctgaagga
    11461 atgaaacaat ccgttttagc tcacctacaa ttttttaaca ctacgccgta tacagcgcct
    11521 tacattattg gtatgaatgt tggaattgaa aaagaagaaa aaaataagtc tttggaagca
    11581 gtaagtgctc tgaaaacagg tctaatgggt cctttagcgg gaattggaga tagtgtattt
    11641 gtagttattc catggactat ttttggagca attgcagcta atatggcatt ggaaggtagt
    11701 tttgttggaa ttattttgtg gatactggca tcaattttat taaggattac tgcttatcct
    11761 ttataccttg ctgggaaaga atcgggtgcg agcctactaa ctcaaattga aacaaaatta
    11821 aagtctatta ctgatgcagt atcaatttta gggttaatgg tggtcggtgc actcattgcc
    11881 acagttgtaa aagtgaatgt tggctttgaa tttaaacaag gcgacttaac aatgaccgga
    11941 aatgaaatct ttgatcaagt tatgcctggt ttattacctg catgtgtagt agcaattgtg
    12001 tattatttgt taggacgaaa ggttaaacca gtttatgtaa ttctattaat cttagtagtt
    12061 tctattgcgt taagagcgtt aaatattttg aagtaggtgt tgaatatgga tgaaaaaatg
    12121 aaaggtatta tacatgttcg tattgatgat cgaatgattc atggacaagt agcaacacaa
    12181 tggacaaatc aattgagtgc taacagaatt atggttatta ataatgaagt agccaatgat
    12241 gacgtaaaaa aagctgtagt tcgcctcgct gcaccaccta atgttagaac ttcaataatt
    12301 acaagggaag tagcagttaa aaatattctt tctggtaaat atgaaggaca aaaagttctt
    12361 attgttgcag tttctccaat cgatgttaaa tttttgattg ataacggttt accaataaca
    12421 agtgtaaacg taggaaattt atcaagaagg aatggaacag aacgcatccg tccgacgata
    12481 aatattactg aagaagagag agaggctttt aaagaactaa ttagtgatgg agttgaagtg
    12541 acagtgatac aaactcctag agattcaaaa gaatttttaa aggattttat ttattaagaa
    12601 cagaggatga gtggatggag tttataatta catctcatgg aaatattgcc attggatttg
    12661 tagatacatt aaaaatgttt tttggcgata atttaaattg tcatgtggta acacttggag
    12721 atgcaggcat tgaagaattc cgtgaaaaca ttgaaaaaat agtttctcaa gtaaaaaatc
    12781 aagaagttat ggtatttgct gacttgattg gtggaacacc tttcaacgaa tttgctaaaa
    12841 gaagtgaagt atttcaaaat gggtttcaac ttcttggtgg catgaattta ggaattttga
    12901 ttgaggctgt caatttgaga ttacaaggaa aaaaaagcga tgatatcatc gaaaatctaa
    12961 gaaatatgaa tacaattgcg tgtcttgcag atatgcaaag acatactaac gaagaggatg
    13021 agtgataaat gcaaaaaatt ttttacaagc ctaaagattt ttggtttggg gattgcatgc
    13081 ctttttataa agatggaact ttttatctat ttcatcaacg agatacgaga aatcctaaac
    13141 ctttaactga tccttttggg tggtcactag ttacaaccac tgattttgtg aaatttgaag
    13201 aatacgggga agtaattcat cgtggtggca acgatgacat tgatcaatac atttacgctg
    13261 gaagtattgt ccaaggaatc aatgagtatt tagctttgta tactggacat aatcgcaaag
    13321 ctaagttgga tggaaaaact tcagaagtgt taatgattgc aagttctaaa gatttaattc
    13381 attggaaaaa ggatggcgaa gcagcaaaac ttgttcctca agaaggatat gataagaatg
    13441 attggcgcga tccttttgca atttttgatc aagatcatgg aaaatacgtt ttgatactag
    13501 gagctagaaa accagcaagt aagaaaaagc cgacggggag attggtctat tttgaatccg
    13561 aagatttaaa agattggaat ttcaaaggag actactgggc taataatgaa tttaacatga
    13621 ttgaaatgcc tgatatattt gaaattgatg gaacgtggta tttacttttt tcagagtatt
    13681 gtgaagataa aaagactaaa tatcgagtcg gagcaggatt attttcagat tggaaatcac
    13741 cagtagacga agctttcgac ggaaaagcct attatgcagc aagaacagtc ggaactaatg
    13801 aaactggccg ttttctaact ggctgggtgg caacaaaaga aggggagaac gatcaaaata
    13861 actgggattg gggcggtgca cttgttccac atcaaattat tcaaaagaca gacaaaactc
    13921 ttggtgttaa actgcctgaa aaaataattg agaattttaa agaaatcact ccattttcaa
    13981 gttttaagtt agagaaagaa tttggaaaaa aagaaagagt aattgttcct aaaatcgaag
    14041 attattttct atttgaaaca actttgacta ttacatcgtt aacaaaggag tttgctttaa
    14101 aattatttgt agatagtgat actcaagaag gatacgaatt tcgtatttca ttgattaata
    14161 atcagcttac tttcggaaaa actccgaact atccttggcc tcaaatgttt gataaggggc
    14221 tgcaacgacc actatttttg gaaataggca aagaatatga actaaagtta attgttgatg
    14281 atacgatagc tgttttatat attgataatg ttgctttaag tactagaatg tatgataaag
    14341 tcggaacagc attaagtatg actgttacgg agggagcaat caaggtagat aaactagact
    14401 attcaataaa ttatgaatgt ggattttaaa acaattgaag ggatgatgta agtgaaatta
    14461 aatggtttac aacatattgg aattccaaca aaagattttg gagcttcaaa aatcttttat
    14521 gaatccttag ggtttagtct gattaatgaa gaaatgaatg tgggaagtaa agtcggcttt
    14581 ttcgagttga atggaacgat aatagaaata tgggaagata aaactactga gtgtagagga
    14641 gccattgatc atctagcatt agatacggat gacatagatg aggtattcaa ttcaattcaa
    14701 gatttaggat atgaattatt agataaaaat attatggaat taccgttctg ggaaaatgga
    14761 atccgttact ttaatttcta cggaccaaat catgagatta tagagataag ccaaatgaag
    14821 aaataaatag ataacgaagt gctctaagtt ggtatgaacg aaaagtactt cattttttgg
    14881 tatcagcatt tcaatagttt aatgagaatt tactatttca aattaattca aagtgacagt
    14941 attttttact tatttggttt caaattttgt aatccttcct atttattgga atacactcca
    15001 cattttgtat acaagtcata tcatgtttta gcaaagctct taccaagcaa agttataatg
    15061 atgttttcca aagtttgatc tttcgatgga ggctaacaca tattttttgc aggacagcca
    15121 aaattagatg tatagaatag agtctgtgag gcgtatagac agtttattgt ctgtatgcct
    15181 ttttttattt tgaaaaattt ttagtatttt tcgaaaaatg ctcactccta gagtaagcgc
    15241 cccatagaac agtaccttac taacaaatgc acctcaagat atacttgagc tatcaagaag
    15301 tctggaggtt ttct
//
LOCUS       DAZKLM010000062        15255 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3764
            SAMEA115706913-rid22582563.denovo.064, whole genome shotgun
            sequence.
ACCESSION   DAZKLM010000062 DAZKLM010000000
VERSION     DAZKLM010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706913
            Sequence Read Archive: ERR13196647
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15255)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15255)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287823.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287823.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,004
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,849
            CDSs (with protein)               :: 2,849
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 41 of 96
            Pseudo Genes (incomplete)         :: 52 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 15 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15255
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706913-rid22582563.denovo.064"
                     /isolate="VRE3764"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            2..1021
                     /locus_tag="ACG842_001878"
     CDS             2..1021
                     /locus_tag="ACG842_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HFQ0248412.1"
                     /translation="MSIITIFFICVLTSWVIQHQAALYEVPVIEIEKVRIKEDHQLIT
                     GKLMNSDQKGESIYLSVPYQKNQIETANIKARQMYLVQMHGDEWQLVAKKNDGWLFFF
                     TSIFIGAMLLIGGKQGLFALGSLIINIVLLLLLLLIFTKTEGISLLNKSILFVVIGMI
                     ISILALDGWNRSSIIKISAAVTSVFLAFFICLLTMNHWKDQGLRYEELNYLTRPYRSV
                     FLSSVLIGTAGGTLDTVITVIATLEELTKRQPEITAKQLWLSGRQVGRDVIGSMTNIL
                     LFVYISGSVPSLLLYLGNQWSFKETIEQHLSLEFLRVIAGSLGIVLSIPISVLFFLTV
                     RRLKR"
     gene            1018..1785
                     /locus_tag="ACG842_001879"
     CDS             1018..1785
                     /locus_tag="ACG842_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HFQ0248413.1"
                     /translation="MNVLIILVGILAISVLLIGGTQGMYILLGLFINLGIFFLLLFGY
                     HQKWPILALSVIGFLLIAVVILFFINGYNLKMRAAFVSILIFLFCFLLLLPITDFLAI
                     QGFTSVELEELSGLDKTLAIDFRLLTRSLLLISLSGAVLDASVAISSGTFEVYQANPQ
                     LSFYQLRHSSFAIAKKILASTVMTLLFAFMGSSLALILWFIDLDIPFQQVINEKSFVL
                     EYTMAILTTLSALIVLPLTCVTTAYLFTKKKEQLKME"
     gene            1885..3453
                     /gene="liaX"
                     /locus_tag="ACG842_001880"
     CDS             1885..3453
                     /gene="liaX"
                     /locus_tag="ACG842_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="daptomycin-sensing surface protein LiaX"
                     /protein_id="HFQ0248414.1"
                     /translation="MKERERILDLVKKGILSTEEGLDLLESMATEKDEKQIKKEADKV
                     TASHKEKDKASQLIDKLENGEEEISEPVDPKERERQDQENLEKILDELATEANKTSAR
                     LDEVNAQFADNKAARNEKQEALMQLNTKEELGELTEEELAQRQALEAEIKELEASGDT
                     LLEEQLKLEAELKDIRKNQWSEKKETFTDKFELPDDWKDQATDTLNQVGEKMSEAGSQ
                     LGKFLKKTFQTVSETVNDNMEWKDVSLRVPGIATTKFEHEFYYEAPAASILDIKAANG
                     NVTLKTWDSNDVKVEAKIKLYGKMGAEPFEAFSERSQIEVNEDHISFQIPNKRVRADL
                     VFYLPKRVYDHAAIKLLNGNIMIETLEAKDIYTKSTNGNIIVDQLTATMLEVEGVNGN
                     IDIRNGNILDSIIETVNGTVTFGATAENLSVSLVNGDVRLTIKEDNLKKVEASSVNGN
                     VKVALPDGIGLEGHAKTSLGSINSRLSNYEVVREKKERTNQMLQFRRVSDDEIAQVQL
                     STTTGSIYLKDTDK"
     gene            3750..4070
                     /locus_tag="ACG842_001881"
     CDS             3750..4070
                     /locus_tag="ACG842_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0080135 - regulation of cellular response
                     to stress [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HFQ0248415.1"
                     /translation="MMKKKLTKSNKNVVLTGTLAGIGEYIGIDPTVIRVLYVFLSLVA
                     FGSPIILYILLALIIPSARSARTNYGHNNAYYEKNNYREQTREQTKKRKEAEKIDDDE
                     WSDF"
     gene            4070..4420
                     /locus_tag="ACG842_001882"
     CDS             4070..4420
                     /locus_tag="ACG842_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HFQ0248416.1"
                     /translation="MSYFQRILVNTLTFISLAVLFPAMVHVSSIWTAVLASFVLSVLN
                     MFVKPILTILSLPFTLVTLGLFTFVINAAILKMTSFFVGAEHFSFSSFGAALLVAILM
                     SLVNVIVSEHNSRK"
     gene            4587..5525
                     /gene="hprK"
                     /locus_tag="ACG842_001883"
     CDS             4587..5525
                     /gene="hprK"
                     /locus_tag="ACG842_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006109 - regulation of carbohydrate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr(Ser) kinase/phosphatase"
                     /protein_id="HFQ0248417.1"
                     /translation="MSEVVKVSQLVKKLSLEIVTGDEKSLDRVIVTGDISRPGLELTG
                     YFNYYSHNRLQLFGSKEISFAERMIPEERLIIMRRLCSEDMPAFIISRGLPAPEEMIQ
                     AANENGIAVLRSPISTSRLLGELSSYLDGRLAPRTSVHGVLVDVYGLGVLIQGDSGIG
                     KSETALELIKRGHRLIADDRVDVYQQDELTVIGEPPKILEHLIEIRGIGIIDVMNLFG
                     ASAVRGSMQVQLAVYLEAWAKDKKYDRLGSEDTTVEIAEVGIPQIKIPVKTGRNVAII
                     IEVAAMNFRARTMGFDATKTFEERLSRLIEENSGND"
     gene            5540..6370
                     /gene="lgt"
                     /locus_tag="ACG842_001884"
     CDS             5540..6370
                     /gene="lgt"
                     /locus_tag="ACG842_001884"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HFQ0248418.1"
                     /translation="MLAQINRVAFELVGIPIYWYALIIVSGIIIAMWLSSREAVRVGL
                     KADDVTDFMLWGLPISIIGARLYYVLFDLPQYIADPIQIFNIRSGGLAIYGGLIAGAI
                     TLYFFTKYHFISLWTFLDIAAPSVLLAQAIGRWGNFMNHEAYGPDTTRSFLESLHLPQ
                     FIIDNMYIEGIYRQPTFLYESLWSLIGFILLLFLRKKPHLLKKGEVLLCYLMWYSFGR
                     FFIEGLRTDSLYFLGVIRISQLLSAVLFVGTLILFIWRRKKGNLPDYDRSLGKNQQII
                     "
     gene            6414..7439
                     /locus_tag="ACG842_001885"
     CDS             6414..7439
                     /locus_tag="ACG842_001885"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046168 - glycerol-3-phosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HFQ0248419.1"
                     /translation="MNYRVAVLGPGSWGTALAQVLAENGHDVRIWGHRASQVEEINTQ
                     HTNQRYLPDHYLPDSIQAFTNMEDAVKDADAVLFVIPTKAIRSVAKELVPKLNTKPVI
                     IHASKGLEQGTHKRISDILLEEIPAEKRKAVVVLSGPSHAEEVAKHDITTITAASTDA
                     KAAKYVQELFMNNYFRIYTNPDVIGVEMGAALKNIIAIGAGAIHGLGFGDNAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGIGDLVVTCTSVHSRNWRAGNLLGQGQPLEEVLA
                     NMGMVVEGVNTTQAAMELAQMMEIEMPITQTIYEVLYEQKEIKTAAKEIMLRDGKKEN
                     EFSLTIF"
     gene            7454..8392
                     /gene="galU"
                     /locus_tag="ACG842_001886"
     CDS             7454..8392
                     /gene="galU"
                     /locus_tag="ACG842_001886"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006011 - UDP-glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HFQ0248420.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LKSGIEDILIVTGKAKRPIEDHFDSNIELETNLSEKGKTELLKLVEETTDVNLHFIRQ
                     SHPKGLGHAVLQARAFIGNEPFVVMLGDDIMEDEVPLTQQLIDDYERTHASTIAVMQV
                     PHDETSKYGIINPGEVLEDGLYNVKNFVEKPDPSKAPSDLAIIGRYLLTPEIFDVLSE
                     QQPGAGNEIQLTDAIDTLNKTQRVFARKFTGKRYDVGDKFGFMKTSIEYGLTHPEVGA
                     PLREYIMSLGKTLEAEGSKINHNPKGITEIKEEKKK"
     gene            8517..8957
                     /locus_tag="ACG842_001887"
     CDS             8517..8957
                     /locus_tag="ACG842_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HFQ0248421.1"
                     /translation="MSGGEIAGLIAAIAFAILVVFIVRVLLQVSKTLEKVDQTVAEAN
                     TTIEIVTQDVDLLSRQVEGLLVKSNELLLDINGKVATIDPLFTAVADLSESVSELNHS
                     SKNIVTKVGGIGKTTAKATVAGKVGGTAMKFFKNKKHTETTGGK"
     gene            8960..9562
                     /locus_tag="ACG842_001888"
     CDS             8960..9562
                     /locus_tag="ACG842_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HFQ0248422.1"
                     /translation="MAKKGGFLLGAVVGGTAAAIAALLLAPKSGKELRDELAAQADDL
                     MDMASDYTDMAKEKSSELTGIAKEKAQGLSQQAGDLAGNVKQKVSESAEEAGSESDDI
                     LLNLKEQSADLSNRFKKTAEDLKKPADELGSIAKDTSEDIMIDVKDAADKAGETVNEG
                     MSEAKPVADDTKEMLKMAKEDAKEAKKEMKDTMTDSTTNH"
     gene            complement(9625..10728)
                     /locus_tag="ACG842_001889"
     CDS             complement(9625..10728)
                     /locus_tag="ACG842_001889"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HFQ0248423.1"
                     /translation="MNNVKINELRQWMAQHQVELAYISDPGHIAYFSGYESDPHERVL
                     ALFIPLEQDPFLFTPALEVEDAQNSSWAYDVRGYLDSENPWEIIGKELRARYGQPGNV
                     GIEKNALTVDRFDAISDILTGTDSYTDLTSIIQRLQLIKTVEEKDKLIEAGKWADVAF
                     EIGFKTVKEGVTEQAIIAEIEYQLKKQGVSQMSFDTLVLAGANAASPHGTPGSTKVSP
                     NELVLFDLGVIWNGYCSDATRTVAYQKPTEFQEKIYNITLEAQLAAQEAVRPGVTAGE
                     LDQIARNVINSYGYGEYFNHRLGHGIGTTVHEFPSLVEGNDLVIEEGMCFSLEPGIYI
                     PEKVGVRIEDCVYVTSDGCVPFTTTPKELLVLE"
     gene            10938..11939
                     /gene="ccpA"
                     /locus_tag="ACG842_001890"
     CDS             10938..11939
                     /gene="ccpA"
                     /locus_tag="ACG842_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001228 - DNA-binding transcription
                     activator activity, RNA polymerase II-specific [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HFQ0248424.1"
                     /translation="MEKQTITIYDVAREANVSMATVSRVVNGNPNVKPATRKKVLEVI
                     DRLDYRPNAVARGLASKKTTTVGVIIPDVSNMFFASLARGIDDVATMYKYNIILANSD
                     GNDQKEVNVLNNLLAKQVDGVIFMGHHITDEIRGEFSRSKTPVVLAGSIDPDEQVGSV
                     NIDYVSATKDAVNKLAKSGNKKIAFVSGALIDPINGQNRLKGYKEALSENNLPYSEGL
                     IFEAQYNFKDGLSLVDRIHNSGATAVYVSDDELAIGILDGLLDRGVKVPEEFEIITSN
                     NSLLTEVARPRLTSITQPLYDLGAVSMRLLTKMMNKEEVEEKTIILPYGIEEKGSTK"
     gene            12294..12689
                     /locus_tag="ACG842_001891"
     CDS             12294..12689
                     /locus_tag="ACG842_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0248425.1"
                     /translation="MPKKRRKTIVIILTILTIGIGGKFYMDKREMQRQKDLLAVEKQS
                     VKVLKNTFADIKEVKIEKSARNEMTGSYRIVILMTNKQDQSIYFSYSFWKERNEIGSY
                     GIVDEKKQKEGNTLNKVKVTYSNGNEESI"
     gene            12689..12940
                     /locus_tag="ACG842_001892"
     CDS             12689..12940
                     /locus_tag="ACG842_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0248426.1"
                     /translation="MITDKQYSELSDAVYWLDPKHRDYVPEMQENLSFKINGTMYKIL
                     KIKNSFDGMQAMAVAPIVHSKLEKNFKNKKIPANFRVLK"
     gene            12983..13378
                     /locus_tag="ACG842_001893"
     CDS             12983..13378
                     /locus_tag="ACG842_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0248427.1"
                     /translation="MSRKIRKLSLAILLIVILGIGGKVYMDKREAQRQKDLLAVEKQS
                     VKVLKNTFADIREVKVEEFKKNPVTGSYGALVTMTNNEGKSVYFDYSFWKERNELGGF
                     GIENRAVQKTGVTIKRVKVIFSNGQEELV"
     gene            13375..14631
                     /locus_tag="ACG842_001894"
     CDS             13375..14631
                     /locus_tag="ACG842_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipase"
                     /protein_id="HFQ0248428.1"
                     /translation="MTINDKHYNDISEKVYWLDPKYPRYNEGYKKNSVKEFAGMEFQI
                     LQIKDSLDGMQAMSVAPIKNGKVDLSQVVIAFAGTNLSDWKDLETDARSIMNLYGNRP
                     GASFSEKATTNKILSVSGLKTAGQINSAIAFANEVKKEYGEAAISTTGHSLGGFLALY
                     VAAEKQWKNVGFNGPDPYDLLSPEAKKWVEENPGMLTNYRNHADFIGNFGGNGTGAEV
                     RISMETAFLNLLASHSLTSWKFDENGKLIIPENENNVKARKQQKENQATQLFSYQLQE
                     LNNIRKKLRLSGGRLTVNEQIYLDDTQARLVVEKLSAKMKSAMEEVIRINQRGIFELE
                     KKWQEGLRQARVVAPSLTEQEIIEALASVGATKENLVDSAKEVYLLRINKARRMGEAF
                     DTLAKEIQDKINEIVARDRELAQQFN"
     gene            14643..14966
                     /locus_tag="ACG842_001895"
     CDS             14643..14966
                     /locus_tag="ACG842_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0248429.1"
                     /translation="MKLEELERDYRSERRQLEAQEDELIHFKNQGLRLMEQEEQRLHH
                     VLKNLALDNEPLFQARRSYSNYQEHYQQALQVERRKLYQQQDELERSYRQKIRQITME
                     KSSYE"
     gene            14959..15225
                     /locus_tag="ACG842_001896"
     CDS             14959..15225
                     /locus_tag="ACG842_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0248430.1"
                     /translation="MNEFLMQLLKELEKNLHRSNVRSVGMGISQQIWAEKRLTDQNYQ
                     MTAEEAAQEFSNRHVHLKGSVKGQFAEKLTELFQRQEKKLKQFR"
BASE COUNT         5242 a         2580 c         3225 g         4208 t
ORIGIN      
        1 aattagcatc atcactatat tttttatttg tgtgcttact tcatgggtta ttcagcatca
       61 agccgcttta tatgaagtac ctgtcatcga gatagaaaaa gtacgaataa aagaggatca
      121 tcaattgatt acaggaaaac tgatgaactc ggatcaaaaa ggagaatcga tttatttatc
      181 ggttccttat caaaaaaatc aaatagaaac agcaaatata aaagcgagac aaatgtatct
      241 agtccaaatg catggagatg aatggcagct agtagcaaaa aaaaatgatg gctggctttt
      301 cttcttcaca agtatcttca ttggcgccat gttgcttatt ggcggaaaac agggtctatt
      361 tgctttagga agtctcatca tcaatatcgt tttactttta cttttgttgc tcatctttac
      421 taaaacagaa gggatttctc tattaaataa atctatcctg ttcgtcgtta tagggatgat
      481 tattagcata ttggctttag atggatggaa tagaagcagt ataatcaaaa tttcagctgc
      541 agttacgagt gtttttttag cattttttat ttgtcttttg acaatgaatc attggaaaga
      601 ccaaggactg cgctatgagg aactgaatta tctgacacga ccgtatcgtt ctgtttttct
      661 ttctagcgta ctgattggaa cagctggagg aacactcgat accgttatca cagtcattgc
      721 gacattagaa gaattgacta agcgacaacc agaaataaca gcgaaacagt tatggctttc
      781 aggaaggcaa gtgggtcgag atgtgattgg ctcgatgacc aatatccttt tgttcgtcta
      841 tatcagtggg tcagttccat cgcttctttt atatttagga aatcaatggt ctttcaaaga
      901 aacgatagaa cagcatttat cattggaatt tttacgcgtc attgctggca gcttaggaat
      961 agtattatcg atccccatct cggtcctctt ttttctgact gtaaggaggc tgaaacgatg
     1021 aatgtactta ttatacttgt tgggatttta gcaatcagcg tgctacttat tggcggtacg
     1081 caaggtatgt atattctttt aggcttattc atcaatttag ggatcttttt cctgttgtta
     1141 ttcggttatc atcagaagtg gccgatactg gcattgtcgg tcataggatt tttattgatt
     1201 gctgtggtta tcttattttt tatcaacggt tataatctaa aaatgcgtgc agcttttgtg
     1261 agtatactga tctttttatt ttgtttccta ctattgcttc ctatcactga ttttttagcg
     1321 atccaaggtt ttacatcggt agaactagaa gaattatctg gcttagataa gactttagcg
     1381 attgatttta gattacttac gagatcgttg cttctgatta gtcttagtgg tgctgtttta
     1441 gatgcgagtg tagcaatttc tagtgggaca ttcgaagtat atcaggcaaa tccgcaatta
     1501 tctttttacc aattgcgcca ttctagtttc gctattgcta aaaaaatatt ggcgtcaaca
     1561 gtaatgacac ttctttttgc attcatggga agttctttag cgttgatcct ctggtttatt
     1621 gatttagaca ttccttttca gcaagtgatc aatgaaaaat catttgttct agaatatacg
     1681 atggctattc ttaccacatt gtccgcacta attgttcttc ctttaacctg cgtgactaca
     1741 gcatatctct ttacaaaaaa gaaagaacaa ctcaagatgg agtaaaagtt acaacttaag
     1801 tcctatgaca tttttactga atcagtgcat aatatggatg taaggaaaag cttgcaaaca
     1861 attttgctta aggaggcaat ttccatgaaa gaaagagaac gtattttaga tttagtgaaa
     1921 aaaggaatcc tttcgactga agaaggctta gatttattag aaagcatggc aactgaaaaa
     1981 gacgaaaagc aaatcaaaaa agaagcagac aaggtaactg ctagtcataa agaaaaagat
     2041 aaggcaagtc aattgatcga taaacttgaa aacggcgaag aagaaatttc tgagccagtt
     2101 gatccaaagg aaagagaaag acaagatcaa gaaaacttag aaaagatctt agatgaacta
     2161 gcaactgaag cgaataaaac ttcggcgcgt ctagatgaag taaatgctca attcgctgac
     2221 aataaggcag cacgcaatga gaaacaggaa gcattgatgc agctgaatac aaaagaagaa
     2281 ttaggcgagt tgacagaaga agaattagca cagcgtcagg cgttagaggc agaaatcaaa
     2341 gagttagaag ctagtggcga tacactgtta gaagaacaat tgaaattaga agcagaatta
     2401 aaagatatcc gtaagaatca atggtctgag aaaaaagaaa cgtttactga taagtttgaa
     2461 ttgccagatg attggaaaga tcaagcaaca gatacactga atcaagtagg cgaaaaaatg
     2521 tcagaagctg gatcacagtt aggcaaattc ttgaagaaaa cattccaaac tgtttcagaa
     2581 acagtgaatg acaacatgga atggaaagat gtcagcttac gtgtccctgg gattgcgaca
     2641 acgaaatttg agcatgaatt ttactatgaa gctcctgcag caagtatcct tgatatcaag
     2701 gcagcaaacg gaaacgtgac gttaaaaacg tgggacagca acgatgtgaa agttgaagct
     2761 aagatcaaac tttacggaaa aatgggtgct gaaccatttg aagcattctc tgaacggagc
     2821 cagattgagg ttaatgaaga tcatatctcc ttccaaattc caaataaacg tgtgcgtgca
     2881 gaccttgttt tctacttacc taaacgagta tacgaccacg cagctattaa acttctaaat
     2941 ggcaacatca tgattgaaac attggaagct aaagatattt atacaaaatc tacgaatggt
     3001 aacatcatcg tggaccaatt aactgcaacg atgctggaag tagaaggtgt aaacggcaat
     3061 atcgatattc gaaacgggaa catcttagat tctattatcg aaacagtaaa tggtacagtg
     3121 acatttggtg cgacagcaga aaatctttct gtgtctttag taaatggtga tgtacgtctt
     3181 acgattaaag aagataattt gaaaaaagta gaagcaagtt cagtgaacgg aaatgtcaaa
     3241 gtcgctttac cagatggcat cggtttggaa ggacatgcta aaacaagtct tggaagtatc
     3301 aatagccgtc tgtccaatta cgaagtagtt cgtgagaaaa aagaacggac aaaccaaatg
     3361 ttgcaattcc gtcgtgtttc agatgatgag atcgcgcaag tgcaactttc aacgacaaca
     3421 ggaagtatct atttaaaaga tacagataaa taaagaggtt gtgaaagaga tgttcagcct
     3481 tgagcaataa gaagcgattt tgtaaaatag cttcccctat tttacaaaat tgtgccttat
     3541 tgccgatagt ctatctcttg aacaccgttt accaagagat tatgaacctg ccaattatcg
     3601 ccgagtcaca aggaataatt tcagaaaaag ctcgtcacgt tttatgaata tggtaatttg
     3661 tgaccgagaa ttttgcagaa agacaccgat taagaagaag gttgtcacaa cgattttctt
     3721 catttaaaat agtagaacag aggtgagaca tgatgaaaaa aaagttgaca aaatctaata
     3781 aaaatgttgt tttgacaggg actttagcag gaataggaga atacatcggg attgatccga
     3841 ctgttatccg tgtgctttat gtattcctga gcttagttgc ttttggaagt ccaattattt
     3901 tatacatttt attagctttg attatcccat ctgcgcgttc tgcacgaaca aactatggac
     3961 ataacaatgc atattatgaa aaaaacaatt atcgtgaaca aactcgtgaa caaacaaaaa
     4021 aacgcaaaga agcggaaaaa atcgatgatg acgaatggag tgacttttaa tgtcgtactt
     4081 tcaacgtatt ttagttaata cgttaacatt catatcattg gctgttcttt ttcctgcgat
     4141 ggtccatgtg agcagtattt ggacggccgt tcttgccagc ttcgtattgt ctgtgttgaa
     4201 tatgtttgtc aaaccgattt tgacgatact gtccctgccc tttacattag tcacattggg
     4261 attgtttacg tttgttatca atgcagcgat tcttaaaatg acctcctttt ttgtgggagc
     4321 agaacatttt agtttttctt cattcggtgc agcgttgtta gtagctatct tgatgtcgct
     4381 tgtcaatgta attgtcagcg aacataacag tagaaaataa aagaaagctg tgccgaacta
     4441 tcggcgcagt ttttttattt agaatcagga atgcgttttc tattacattg gtaattaaat
     4501 aaaaacgttt tttttaattg gtagtacttt tggctagaca atgataaaat gatagagaac
     4561 actctaatag aaatgaggta gcatgaatgt cagaagttgt aaaagttagc caattagtga
     4621 aaaaattatc tttggaaatc gtgacaggcg acgagaaaag cttagaccgt gtcatcgtaa
     4681 ctggggacat ctcacgccca ggactagagt tgactggtta ttttaattat tattcccata
     4741 atcgtttaca gctatttggc agtaaagaaa tcagttttgc ggaacggatg atcccagaag
     4801 agcgtttgat catcatgaga agattatgta gtgaagatat gcccgcattt attatttcaa
     4861 gaggattacc agctccagaa gaaatgatcc aggcagcaaa tgaaaatggg atagctgtac
     4921 tgcgttctcc catctcaact tctcgtttat taggagaatt atccagttat ttagatggtc
     4981 gccttgcccc tcgaacaagt gtccacggtg ttttagtgga tgtttacgga ttaggtgtac
     5041 tgattcaagg tgacagtggg ataggaaaaa gtgaaacagc gttggagttg atcaaacgcg
     5101 gccatcgatt gatcgcagac gatcgagtag atgtttatca gcaagatgaa ttaacggtga
     5161 tcggagaacc gcctaaaatc ctggaacatc tgattgaaat ccgaggaatc gggatcattg
     5221 atgtaatgaa tctttttggt gcgagtgcgg tacgtggctc tatgcaagtc cagttggctg
     5281 tttatctgga agcatgggca aaagataaga aatacgaccg tctgggctct gaagacacga
     5341 ccgttgaaat cgcggaagtg ggcattccac aaataaaaat ccctgtcaaa acaggacgaa
     5401 acgttgcaat catcattgaa gtagccgcga tgaatttccg tgcacgtaca atgggctttg
     5461 acgcaacgaa aacatttgaa gaaagattaa gtcgcttgat tgaagaaaac tcaggcaacg
     5521 actagcaagg agtgacaata tgttagcaca aatcaatcga gttgcttttg agcttgtagg
     5581 gattcctatc tactggtatg cattgatcat cgtatcaggt atcattatcg ctatgtggct
     5641 tagcagcaga gaggctgttc gggtaggatt aaaagcagat gatgtgacag attttatgtt
     5701 gtggggattg cctatctcca ttattggcgc tcgcttatat tatgttttat ttgatcttcc
     5761 ccagtatata gctgatccaa tccaaatatt caatatccgt tcaggaggct tagctattta
     5821 tggcggactg attgcaggcg cgatcacgct gtattttttt actaaatatc attttatttc
     5881 tttgtggact tttttagata tcgctgcacc aagtgtatta ttagcacagg caatcggtcg
     5941 ttggggaaat ttcatgaacc acgaagcata tggaccagac acgacgcgaa gcttcttaga
     6001 aagtctgcat ttaccgcaat tcatcattga taatatgtat atcgaaggga tctatcgtca
     6061 accaacattt ctttatgaat cactttggag tttgattgga tttattttgc ttttgttttt
     6121 acgaaagaaa ccacaccttt tgaaaaaagg cgaagtcctt ttatgttatc taatgtggta
     6181 tagtttcggg cgtttcttta ttgaaggatt acgtacagac agtctttact ttttaggtgt
     6241 gatccgtatt tctcaattat tatctgctgt gttatttgtc ggtacattga ttctatttat
     6301 ttggcgcaga aaaaaaggaa atttaccgga ttatgaccgc tcattaggaa aaaatcaaca
     6361 aattatctga tttttagagc gttaaaaatg aaaaaaattt caggagtggt aaaatgaatt
     6421 atagagtagc ggttttagga ccaggctctt ggggtactgc tttggcacaa gtcctcgccg
     6481 aaaacggaca tgacgttcgg atttggggac atcgtgcttc acaagtagaa gaaatcaaca
     6541 cccaacacac gaatcaacgt tatttaccag atcattatct acccgattcc atccaagcat
     6601 tcaccaatat ggaagatgca gtaaaagacg cagatgcggt gctattcgtc attccaacga
     6661 aagcaatacg ttctgttgca aaagaactag tgcctaaatt gaatacaaag ccagtgatca
     6721 tccatgcaag taaaggatta gaacagggaa cacataaaag gatctctgac atcttgttag
     6781 aggaaatccc agcagaaaaa agaaaagctg ttgttgttct ttctggacca agccatgcag
     6841 aagaagtagc aaaacatgac atcactacga tcacagctgc aagtacagat gcaaaagcag
     6901 caaaatatgt acaagagctg ttcatgaata attattttcg gatctatacc aatcctgatg
     6961 tgatcggtgt agagatgggg gcagctttga aaaatattat tgctattggt gctggcgcta
     7021 ttcacggctt agggtttggc gataatgcaa aggcagcaat catgacgcgc ggattagctg
     7081 agatcagccg tttaggtgta gcaatgggtg ccaacccgct gacctttatc ggattaagcg
     7141 gaataggcga tctagtcgtt acttgcacaa gtgttcattc taggaactgg cgcgcaggca
     7201 acttgttagg acaagggcag cccctagaag aggtgctagc caatatggga atggtcgttg
     7261 aaggtgtgaa tactacccaa gcagcgatgg aactagccca aatgatggaa atcgaaatgc
     7321 ccatcactca aacaatctat gaagtgctgt atgaacaaaa agaaatcaaa acagccgcaa
     7381 aagaaatcat gttacgtgat ggaaaaaaag aaaatgaatt ctcattaaca attttttgaa
     7441 ggagcgaatg tatatgaaag ttaaaaaagc agttattcca gcagcaggat taggaacaag
     7501 atttttacca gcaacaaaag ctatggcaaa ggaaatgttg ccaattgttg ataaaccaac
     7561 aatccagttc atcgttgaag aagcgttgaa atcaggtatc gaagacatct tgatcgttac
     7621 aggaaaagct aaacgaccaa tcgaagatca ctttgattca aatattgaat tagaaacaaa
     7681 cctaagtgaa aaaggaaaga ccgaattatt gaaattagta gaagaaacga cagatgtcaa
     7741 cttgcatttc atccgtcaat ctcatccaaa gggtttagga catgccgttc ttcaagcaag
     7801 agcctttatt ggaaatgaac catttgtcgt tatgctaggc gatgatataa tggaagacga
     7861 agtaccattg acacagcaat tgatcgatga ctatgagcgt acacacgctt ctacgattgc
     7921 cgttatgcaa gttcctcacg atgaaacttc aaaatacgga atcatcaacc caggggaagt
     7981 actggaagat gggttataca atgtcaaaaa ctttgttgaa aaaccagatc caagcaaagc
     8041 accaagtgat ttagctatta ttgggcgtta cttattgact ccagaaatct ttgatgtatt
     8101 atcagaacaa caaccaggtg ctggaaatga gatccaacta actgacgcaa tcgatacgtt
     8161 gaataaaaca caacgcgtat ttgcccgtaa attcacaggt aaacgttatg acgttggtga
     8221 caaatttggt tttatgaaaa caagtattga atatggtctg acacatccag aagtaggggc
     8281 acctttgcgt gaatacatca tgtctttagg taaaacatta gaagcagaag gaagcaagat
     8341 caatcataat ccaaaaggaa taacagaaat caaagaagaa aagaaaaaat aatctttttt
     8401 tctaacgagc ttcagctgct aattgaataa aaagtagttg aagctcgttt ttatttgttt
     8461 aaaaaacacg aaaatgctat ccttatagaa aagagagaca agaagggagt ttgatcatgt
     8521 caggaggaga aattgcagga ttgatcgcag caatcgcctt tgcgatactt gtcgttttca
     8581 tcgttcgcgt attgttgcaa gtttccaaaa ctttagaaaa agtagaccag actgtcgcag
     8641 aagcaaatac gacaattgag atcgtcacac aagacgtaga tcttttatct agacaagtag
     8701 aaggcttgct agtcaaaagt aatgagctgc tcttggatat caatggaaaa gtcgcaacga
     8761 tcgatccatt gttcacagca gttgctgatt taagtgaaag tgtatcggaa cttaatcatt
     8821 ctagtaaaaa catcgtgacg aaagtcggcg gaatcggaaa aacgacagct aaagcgactg
     8881 ttgccggaaa agtcggtgga acagcgatga aattttttaa aaacaaaaaa catactgaaa
     8941 cgacaggagg aaaataagaa tggctaaaaa aggtggattt ttattaggtg cagtagttgg
     9001 aggtacagcc gcagcaatcg ccgcattatt gttagcaccc aaatctggta aagaacttcg
     9061 ggatgaattg gctgctcaag cagatgattt gatggacatg gcttcagatt acacagatat
     9121 ggcaaaagaa aaatcatctg agttaacagg tattgcaaaa gaaaaagcgc aaggtctttc
     9181 tcaacaagca ggggatctag caggcaatgt taagcagaag gtaagtgaat cagcagagga
     9241 agcaggaagt gaatcagatg atatcttgtt aaacctcaaa gaacaatcag ctgatttatc
     9301 taatcgtttc aaaaaaacag cagaagatct gaaaaagcca gcagacgaat taggtagtat
     9361 tgctaaagat acttcagaag atatcatgat cgatgtaaaa gatgctgcag ataaagcagg
     9421 agagacggtc aatgaaggaa tgtcagaagc aaaaccagtt gcagatgaca caaaagaaat
     9481 gttgaaaatg gcaaaagaag atgcaaaaga agctaaaaaa gaaatgaaag atacaatgac
     9541 agattcgact accaatcact aagattactc atttctagaa taaaaaaggt gagagcggac
     9601 cagctctcac cttttttatt ttcattactc taaaacaagt aattcttttg gtgtagtcgt
     9661 gaatggcaca caaccatcac ttgtcacata cacacaatct tcgatacgta cccctacttt
     9721 ttcaggaata tagattcctg gttcaagaga gaagcacatc ccttcttcaa tcactaagtc
     9781 gtttccttct acaagagaag ggaattcgtg gacggttgtt ccgattccgt gacctaaacg
     9841 gtgattgaaa tattcgccgt agccatagct gttgatcacg tttcgtgcga tttgatcaag
     9901 ttcacctgct gtcacacctg ggcgaactgc ctcttgtgcg gcaagctgtg cttcaagagt
     9961 gatgttatag attttttctt ggaactcagt tggtttttga taagcaactg tacgagtagc
    10021 atcactgcaa tagccattcc aaataacgcc aagatcgaat aacactagtt catttggtga
    10081 aaccttcgtg cttcctggtg taccatgagg gcttgcagca tttgctcctg caaggactaa
    10141 tgtgtcgaaa gacatttgtg aaacaccttg tttttttaat tggtattcga tttctgcgat
    10201 aattgcttgt tcagttacgc cttcttttac tgttttgaag ccaatttcaa aggcaacatc
    10261 ggcccattta ccagcttcga tcagtttgtc tttttcttct accgttttga tcagttgcag
    10321 acgctggata atagaagtta gatcggtgta agaatctgta cctgtcaaga tatcagagat
    10381 agcatcgaag cgatcgacag tcagagcatt cttttcaata cctacattac caggttgccc
    10441 atagcgtgcg cgaagctctt ttccgatgat ctcccaagga ttttcgctgt ctaagtagcc
    10501 acgtacatcg tacgcccagc tgctgttttg agcatcttca acttccagag caggagtgaa
    10561 aaggaaagga tcttgttcca gagggataaa aagagctagt acacgctcgt gaggatcact
    10621 ttcatatccg gaaaagtaag cgatgtggcc agggtcgctg atataagcca attctacttg
    10681 gtgttgtgcc atccattgac gtagttcatt gatttttacg ttattcattt ggcaacctac
    10741 tttctaaatt aattttgtat tcaataattg taccactgat tgattaaaag accaacgttc
    10801 ttaaaactga aaaaagtaaa aattttcatg aaaacagaga taaaacggtt gcaattgttg
    10861 agattatttg atatcctatt ttagaaatgt aagacgtatt ttcagaaaac atataaaata
    10921 tgaataggag aacacaaatg gaaaagcaaa caatcactat ctatgatgta gcgagagagg
    10981 caaatgtttc gatggctact gtttcacgtg tcgtaaatgg caatccaaac gtcaaaccag
    11041 caacaagaaa aaaagtcttg gaagttatcg atcgcttgga ttatcgtcca aatgcagtcg
    11101 cacgtggctt ggcaagtaag aaaacaacaa cagttggggt aatcattcct gatgtcagca
    11161 atatgttctt tgcctcttta gcacgtggta tcgacgatgt ggcaacaatg tacaaataca
    11221 atatcatctt agcaaactca gatggcaacg atcaaaaaga agtcaatgta ttgaataatt
    11281 tattggctaa gcaagtggac ggcgtgatct ttatgggtca ccatatcaca gacgaaatcc
    11341 gtggagaatt ttcacgttca aaaacgcctg ttgtactagc tggttccatt gatccagatg
    11401 aacaagtagg tagcgtaaac attgattatg tgtcagcaac aaaagatgca gtaaataaat
    11461 tagcaaaaag cggcaataaa aaaatcgctt ttgtcagcgg tgcgctgatt gatcctatca
    11521 atggtcaaaa ccgtctaaaa ggctataaag aagcattaag cgaaaacaat ctaccatata
    11581 gcgaaggatt gatttttgag gcacaataca actttaaaga tggtctgtct ctagttgacc
    11641 ggatccataa tagcggtgca acagctgtct atgtttcaga cgatgaatta gccattggta
    11701 tcttggatgg tttattagac cgcggcgtaa aagtaccaga agaattcgaa atcatcacaa
    11761 gcaacaattc tttactgaca gaagtagctc gtcctcgttt gacaagtatc acacaaccat
    11821 tatacgattt aggagcggta tccatgcgcc tattgacgaa aatgatgaat aaagaagaag
    11881 tcgaagaaaa aacgattatc ttgccttatg gaatcgaaga aaaaggatca actaaataat
    11941 aaaagatcaa tcatttaaag gttgttccaa aagtggtcaa cttctggaac aacgtttata
    12001 cgagaaaggg ggttatgaca aaattttgtc ataacccctt tttttttgtg actttaaaaa
    12061 aagagaaaat ggcggaaaca aaccttacgt ccatcgtata cgacacaatc tgtgcgtaga
    12121 tgctacattt tgttcactac tatcaaaatt actttaaaat gaaaacgtga taaaaaagaa
    12181 agtagaaaag tagataagta actctaaaaa atccaaaaaa atccaggctc gattgcgtac
    12241 ttatcttaaa ggagaaacaa tccatcatat ataacccgaa aggagacttc tatttgccta
    12301 agaaaagacg aaagaccata gtcatcattt tgaccattct tactatagga atcggaggga
    12361 aattttacat ggacaaaaga gaaatgcaaa gacagaaaga tttactcgca gtagaaaaac
    12421 agtccgtcaa agttctgaaa aatacctttg cggatatcaa ggaagtgaag attgaaaaat
    12481 ctgctcgaaa tgaaatgacg ggttcatacc ggatagttat tctaatgact aataaacaag
    12541 atcaatcgat ttatttttct tatagttttt ggaaagaaag aaatgagata ggaagttacg
    12601 gaatagttga tgaaaaaaag caaaaagaag gaaatacgct caataaagtt aaagtaactt
    12661 actctaatgg aaatgaggaa agtatttaat gattactgac aaacaatata gtgaattaag
    12721 tgatgctgtt tactggctag atcctaaaca tagagactat gtacctgaaa tgcaagaaaa
    12781 tttaagtttt aaaattaacg gcactatgta taaaatccta aaaatcaaaa actccttcga
    12841 cggcatgcag gcgatggctg tagcgccaat cgtccatagc aaattggaga aaaatttcaa
    12901 aaataaaaaa attcctgcaa actttcgcgt acttaaataa tgaaggtgtc aatacttaca
    12961 taaaaagaaa ggaggcacgt tcttgtcaag gaaaataaga aaactcagct tagccatttt
    13021 attaatcgtc atattaggga tcggagggaa agtctatatg gacaaaagag aagcacaaag
    13081 gcagaaagat ttactcgcag tagaaaaaca gtcagtcaaa gttctgaaaa atacctttgc
    13141 ggatatcagg gaagtgaagg tagaagaatt taaaaaaaac cctgttaccg gaagttatgg
    13201 tgcacttgtg acaatgacaa ataatgaagg gaaatctgtt tattttgatt atagtttttg
    13261 gaaggagaga aatgaacttg gtggttttgg cattgagaac agagcggtac aaaagacagg
    13321 agtcactata aaaagagtaa aagtaatttt ttctaacgga caggaggagt tagtatgaca
    13381 ataaacgata agcattacaa tgatattagt gagaaggttt attggctaga tcccaaatat
    13441 ccccgctaca atgagggata caaaaagaac tctgttaaag aatttgctgg tatggaattt
    13501 caaatcctac aaatcaaaga ctcattggac ggcatgcagg cgatgtctgt agcgccaatc
    13561 aagaacggta aagtggatct ttctcaagta gtgattgctt ttgcaggaac gaacttaagt
    13621 gattggaaag atttggaaac ggatgcaaga agtatcatga atctctacgg gaatagacca
    13681 ggagcaagtt tttctgaaaa agcaacaaca aacaagattt tatccgttag tggattgaaa
    13741 acagcggggc aaatcaattc agcgattgca tttgctaatg aagtaaaaaa ggaatatgga
    13801 gaagctgcta tttctactac cggccattca ttaggtggat tcttagcatt atatgtagca
    13861 gcagagaaac aatggaaaaa tgtcggcttc aacgggccgg acccgtatga tctcttatca
    13921 ccagaagcaa aaaaatgggt ggaagaaaat cccggtatgc tcaccaatta ccgaaatcac
    13981 gccgatttta tcggtaattt tggtggtaat ggcacaggtg ctgaagttcg gatcagtatg
    14041 gaaacagcat tcttgaatct gcttgcttcc cacagtttaa cctcttggaa gtttgatgaa
    14101 aatggaaagc tgattattcc tgaaaatgaa aacaatgtta aagctcgaaa acaacagaaa
    14161 gaaaaccaag cgacccagtt gttttcctat cagttgcaag aactgaacaa catacgtaaa
    14221 aaactacgat taagtggcgg ccggctgaca gttaatgaac aaatttactt agatgacaca
    14281 caagctaggt tagtagtgga aaagttatcg gcaaaaatga aaagcgctat ggaagaagtt
    14341 atccgcatca atcaaagagg aatttttgag ctagagaaaa aatggcaaga aggcttgcgg
    14401 caagcacgtg ttgtagcccc ttcgttgact gaacaggaaa tcatagaagc attagctagt
    14461 gtgggcgcca caaaagaaaa cttagtcgat tctgcgaaag aagtctatct gctacgaatc
    14521 aataaggcaa gacgtatggg ggaggccttt gatacattgg cgaaggagat ccaagacaaa
    14581 atcaatgaaa tcgtggcacg agatcgagaa ttagcacagc aattcaatta aaggagaagt
    14641 agatgaaact agaagaattg gaaagagatt atcgaagtga acgaagacaa ttagaagctc
    14701 aagaagatga attgatccac tttaagaatc aaggattacg attgatggag caagaggagc
    14761 agcgactgca tcatgtatta aagaacttag ctttagataa tgagcctctg tttcaagcaa
    14821 gacgaagtta ttcgaattat caggagcatt atcaacaagc cctacaagta gaacgacgaa
    14881 aactctatca gcagcaagat gaactagaac gttcctatcg tcaaaaaatc agacaaataa
    14941 ctatggagaa atcatcttat gaatgaattt ttaatgcaat tactaaaaga attagagaaa
    15001 aatctacatc gatcaaatgt acgttcagtt ggtatgggga tatctcagca aatttgggca
    15061 gaaaaacgat tgacagatca aaactaccaa atgactgcag aagaagctgc tcaagaattt
    15121 tctaaccgac atgtacactt gaaaggatca gtaaaagggc aatttgcaga aaaactaact
    15181 gaattattcc aaagacaaga aaagaaatta aaacagtttc gttagtaaag ttatatagaa
    15241 aaattttgtg aaaat
//
LOCUS       DAZKLM010000063        15125 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3764
            SAMEA115706913-rid22582563.denovo.065, whole genome shotgun
            sequence.
ACCESSION   DAZKLM010000063 DAZKLM010000000
VERSION     DAZKLM010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706913
            Sequence Read Archive: ERR13196647
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15125)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15125)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287823.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287823.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,004
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,849
            CDSs (with protein)               :: 2,849
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 41 of 96
            Pseudo Genes (incomplete)         :: 52 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 15 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15125
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706913-rid22582563.denovo.065"
                     /isolate="VRE3764"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..687
                     /locus_tag="ACG842_001897"
     CDS             <1..687
                     /locus_tag="ACG842_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFQ0248431.1"
                     /translation="ILYNVAFILLNLLISVVFAIIMSELRNKRLVKVYQTMSLLPYFL
                     SWVIISYFVYAFLSPDKGIFNQWITGHGGEAVNWYNEPKYWPFILVFIGTWKGIGYNS
                     IIYFASVMGIDPTYYEAAMVDGASKWQQIKHVTIPQLIPLMTILTILAVGNIFRADFG
                     LFYNIPRNSGALYDVTQVLDTYIYNGLTSTGDFGMTAAAGLYQSVVGFVLLMITNTIA
                     RRFDSDSALF"
     gene            699..1619
                     /locus_tag="ACG842_001898"
     CDS             699..1619
                     /locus_tag="ACG842_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0248432.1"
                     /translation="MKKKKVERVQVRSFNKTANFFSNIMIALFAISCILPFIFVIAIS
                     FTDESILSTHGYSFWPEKFSIFGYTYLFDQMQDKIFQALFVTVLVTVLGTLINSTATS
                     LYAYAISRSNFPFRRFFTVFCLITMLFSPGMVANYLVMTNLLQLKDTIWALILPMAVS
                     PFNIIVMRTFFKRSVSDSIIESARIDGASELRIFVQIVLPLAVPGIATISLFAALGYW
                     NDWFNALLYIQDDKLVPLQYLLMKIQSNIQYLTQNSGAGSQLTGGLASVPGESARMAI
                     VVISTLPIAISYPFFQKHFVKGLTIGGVKE"
     gene            1660..3117
                     /locus_tag="ACG842_001899"
     CDS             1660..3117
                     /locus_tag="ACG842_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFQ0248433.1"
                     /translation="MKLWKKLAFTGVSALLLGTLAACGGSGSKDQAEASNSDTLQMYQ
                     IGDKPDNFDQLMEVANKRIEKEIGVKVNINYIGWGDYEKKMNVIISSGENYDIAFANN
                     YVSNAQKGAFADLTELAPKYAEKAYNDLDEAYIKGNLVNGKLYAFPVNGNVFAQQVLT
                     FNKALLDKYDLSIDGIQSYADAEKVLKEFHEKEPNTAAFAIGQGFKVQGNFDYPLGNT
                     LPFAIDLDGDDSKIINQYDNETYIGLLRTMHSWYKQGLIPSDAATSNKDYPLEGNTWL
                     MRGETQGPYDYGDTILTNAAKQELVSKAITVPLKSTAQAQMANFVVSNTSKNKEKSVE
                     LLGLLNSDPELLNGLVWGIEGEAWEKVDGSDDKIKLLDGYQPNTHMSAWNTGNNKILY
                     TQESITDDMIAKRDQSIADAETSPILGFSFNTDSVKTELSNISNVMNQYLDGLNTGTV
                     DPDETLPKLKDALDKAGYDKVLKEMQKQYDEFRQE"
     gene            3462..4076
                     /locus_tag="ACG842_001900"
     CDS             3462..4076
                     /locus_tag="ACG842_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HFQ0248434.1"
                     /translation="MLGKVLEELFIRIWVIIKLTLYFWIYTFAGGIIFGLGAAWKTVN
                     ELFYLYGFEYKEITIKRGWNIYKRNFLRGNLLFSLFLSGTALLSYNLYLSVQIKGLIF
                     LAIDFILLFALLCLFATYQYSMILDSEYTISVPNLLKLSFISVFSSFSSFLKIIIGSG
                     IILGVTWQFKGLILFGVIGLLTVWNGTMTTHWREELDKQLESYE"
     gene            4069..5793
                     /locus_tag="ACG842_001901"
     CDS             4069..5793
                     /locus_tag="ACG842_001901"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFQ0248435.1"
                     /translation="MNKFRILFSKGTLLNRLLRKYAVIMIGVTMTATVIFSIHTWEQN
                     QKQAENMTSDAVQSTSRMLNDKTTLSRIIKNQLVGNSEKIENVTTYLTKPIDQYLMYV
                     YEQQNSTDELVSFPNQIKDLYANYEELSAIYIVLNQLPEYYESSRENKGGEIKKGTPK
                     LLDAFYIKLPITQGGAESGSIFIGFQKKELDMILENLTSFNGLSLYMISGTTNRLYTF
                     HDNKIAKETFERQEMIITESLKKNSQLPIDDLEKNNFVQHQELSDDYRILAVLDKKSV
                     KIETGKNLAPLIIGIIILDNILLIFLYKTFKRYSQQVSIVMQAMDQTAAGNLETRIDI
                     TNTEYELKELSIGINEMLDSINQFVEDIYKLEIKQQDAHMRALQAQINPHFLYNTLEY
                     IRMYAISEGSEELADVVYAFSALLRNNTNQEKTITLKEELDFCEKYVYLYQMRYPNRV
                     AYHFMIDPDLEKIEVPKFVIQPLVENYFKHGIDFTRFDNALSVKVLQEGKRVRIIIKD
                     NGKGMTEKRLKQIEEKLSHPKVELHGSIGLQNVNERLRASFGSSYYMSLENNETGGLT
                     VSITFKEG"
     gene            5790..7241
                     /locus_tag="ACG842_001902"
     CDS             5790..7241
                     /locus_tag="ACG842_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFQ0248436.1"
                     /translation="MSVYQVLLVDDEYMILNGLKKIIDWQSLGFQIVATAENAKEGLA
                     VLEQRQIDLVVTDVTMPEINGLEFIEAAQKERHNFEFMILSGYQEFDYLKGGMQLGAV
                     NYLMKPVNKFELVESLKKIKTRLDQQNEQKNQQEIYQELLFSQWLNEELDEISEEEII
                     GQISEKQHRIVLIQLARTHDTLINRWLQERQQIFYYQRNYGDRVLITLLLEADEVDGF
                     CCFVQENFPDQEWLISIGEETDSLDKIPETFQQAKDNLQLHQFYGDKDEHVFYAVQAE
                     TKDQTIDFSSFRRVLQNKQLEVAQKMITDFFEQFQLAAMMPEDIRYSSFLLFMEIQRE
                     LIDLEDEEYLQGIEKIHQAKTVQELQQLLLSFIKKHQRQKKYSDNVEKVIVILHQHYQ
                     EPLTLKEVSENLHLNVMYLGQLFKKETKKSFSAYLNHLRMEKAKQLLLHSNQNINEIA
                     SEIGYNNTTYFSKLFKKIVGRSPKEYRENSGES"
     gene            complement(7714..9867)
                     /locus_tag="ACG842_001903"
     CDS             complement(7714..9867)
                     /locus_tag="ACG842_001903"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HFQ0248437.1"
                     /translation="MNIGQIDTRHATANQPNFSNGNCQPYTGVPFGMNYFAPQTTDQN
                     GSWWFHPDDRVFQGYRLTHQPSPWMGDFSHMLFTPINGKLQENTLFHAQSSYRPEESI
                     FCPTHLSICQLRYGICSTLIPSMYGGILSIDYSKNDSGLLLSFPGRHQLFVIDPYTVE
                     GKITHFAGSEDPDFSFYFILRFEYPLTIDELAVSNKNSDSIPLYFEQTKKQTIRFGTS
                     FISSEQAAYNLELEQNQSKETYLAHSQEQWQSYFDRIQLTHHDQQQLRTFYHNLYRVF
                     LFPQTFYEIDPSGQKIHYDTTSRSIKPGVLYTNNGFWDTYKTVYPLYSLIAVEKYEEM
                     LEGFLNSYRESGYLPKWLSPDERGLMPGTLIDAVIADAAVKDIRRDLMPEFLTAMEKG
                     ATVQSKHPNYGRQGTEDYLTYGYVPNHYHESVNHTLDYAYSDFCISQVAKVLEEEEMT
                     KKYQQQSLNYQNIFDYESGFMRSKDKDGKFREPFSANRWGQDYAEGSAWQSSFAVYHD
                     FSGLIGKYGGSERYKEKLIELCNQPPTFDPGGYGFEIHEMSEMAAIEFGQLAISNQPS
                     FHYPYLFSYIGQPEMAQPLLKQLMTQTFDDSPMGYPGDEDNGSMAAWYIFNSLGFYPV
                     TPGTGEYVIGMPLIDKAVLLLSNGKELTIEATPNRPQQQFIHKIKRMDKEYTKLYFTH
                     QDLLKGGTISYQLGIIPHPKKYQAKDLPFSLSNYN"
     gene            complement(9869..10915)
                     /locus_tag="ACG842_001904"
     CDS             complement(9869..10915)
                     /locus_tag="ACG842_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFQ0248438.1"
                     /translation="MNQPLYQTIFNDLQTAILTGELPVDSQLPTEKELSRTYKVSRIT
                     SKRALTELEQLGLIYRVRGKGSFVKSASPEQILASPTKTRRILFLLPYVADLSVGDFS
                     KGLNPVMQDKQIDVMMTTLDFLQNKTAKELINEFDGLIYYALDTEQHLDLLFELSLKE
                     FPVVILDKKIYELPFPTVLSDNFQGGALAASYLIEQGHSKIAYLFGTQTHPQSVRQRY
                     LGYLEALDKAGLTFHTSLDDKSATLDELLSYITKYEVTAFVCENDVVAIQTMNLLRQH
                     GYHIPEDFSIIGFDDIQAAALVDPPLTTIAQDFEQLGKLAGDALVTWLETKDRPSDSK
                     FPVSFIKRQSTKEK"
     gene            11065..12354
                     /locus_tag="ACG842_001905"
     CDS             11065..12354
                     /locus_tag="ACG842_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HFQ0248439.1"
                     /translation="MAYKDIPKSVQKFMEDITQKCGDIHADWAKNFTAAFANTLLTTV
                     KKQNDGTTFLLTGDIPAMWLRDSTAQVRPYLVIAKEDEELADMISGLVKKQFFYINID
                     PYANAFNEEANGAGHQTDHTKMNDWIWERKYEIDSLCYPIQLAYLLYKNTGKTDQFNQ
                     SFIEGIKKILDVFKTEQDHEHSPYTFVRDTTRKEDTLINDGRGAKTAFTGMTWSGFRP
                     SDDTCRYGYLVPSNMFAVVVLGYVEEIFQTILDDKEIVQQTQKLKKEIQEGIQNHGRT
                     VNQKGQTIYAYEVDGMGNATVMDDSNVPNLIAAPYLGYCSEQEEDYLQTRRTLLSKEN
                     PYFYEGKYAKGIGSSHTPEKYVWPIALAMEGMTTSDKSEKERILNLLVATDAGTHLMH
                     EGFDVNNPENYTREWFSWANMMFCELVMDYFDIRVEK"
     gene            12367..15069
                     /locus_tag="ACG842_001906"
     CDS             12367..15069
                     /locus_tag="ACG842_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HFQ0248440.1"
                     /translation="MKKKVYIISHSHWDREWYMAYEQHHMRLIELIDDLLELFEVDPS
                     FNSFHLDGQTIILDDYLQVRPEKRQAIQQAINEGKLRIGPFYILQDDFLISSESNVRN
                     MLIGMEESRKWGTPVMLGYFPDTFGNMGQTPQLMKQAGISAAAFGRGVKPIGFDNQVL
                     EAENYSSQYSEMWWKGPDQTAIFGLLFANWYSNGNEIPVEKEAALAFWKQKLADAEQY
                     ASTNHLLMMNGVDHQPVQKDISKAIHLANELFPDYEFIHSNFTDYLEAVQKDVPEDLG
                     SVEGELTSQETDGWYTLANTASARVYLKQWNTKVQRQLENITEPLATMAYEVSGNYPH
                     DQLDYAWKTLMQNHPHDSICGCSVDSVHREMIPRFEKADEVGKYLAQDSLEQLTAAID
                     TTGFPKDSFPFVIVNTAGMDKTGEAEITIELERKRFAEGIPEQLYQELENLPKRKYHV
                     ETKSGATIPAILSEETVQFGYDLPKDRFRVPYMARMIKVTLPLENMPAFSWETFALVE
                     GEAKAEEKETMIHQSGRIIENGPLHLTIEKNGTITMEDRKNKRKLNNLHIFEDIGDIG
                     NEYIFKQPFCDQPILSSNKENSEVKVLVDTPEIAKISILQEMEIPVSADERLEKEQQM
                     VVEFRYRKAERSKEKRILQIKTIMTIRKDSKKIDFETTLDNQMKDHRLRVLFPTKLHV
                     EHHEADSIFEVVKRPNHVSKSWENPTNPQHQQAFVNIHDEEYGVTVGNFGLNEYEVTE
                     DGQIAVTLLRSVGELGDWGYFPTPEAQCLGEHRFNYSIELHGPEEKFSTYLHAYAAQI
                     PFSTQQIKHHEGTLISKQQYLTIKSETFAITALKRSKFSDKVVVRGFNMSSHLEKLEI
                     TKDNGKTVILNLLEEPTKQAVVPIIQPYEIRTIGFEEENECMAE"
BASE COUNT         5096 a         2639 c         3049 g         4341 t
ORIGIN      
        1 attttatata atgtagcatt tatccttttg aatctgctga tctcagtcgt atttgcaatc
       61 attatgagcg agttgagaaa taaacgactc gttaaagttt atcaaacgat gtccttacta
      121 ccttattttc tatcttgggt cattatcagt tactttgttt acgctttttt aagtcctgac
      181 aaaggaatat tcaatcagtg gatcactgga catggaggag aagctgtcaa ttggtataat
      241 gaaccgaaat attggccgtt tatcttggtg ttcattggta catggaaagg gattggttat
      301 aacagcatta tttactttgc atctgttatg ggaatcgatc cgacttatta tgaagctgct
      361 atggttgatg gagctagcaa atggcagcag atcaaacatg tgacgatacc acaacttatt
      421 ccactaatga cgattttaac gattttagcc gtaggaaaca ttttccgagc tgattttggc
      481 ctgttttata acattcctag gaattccggt gcattatatg atgtgactca agttttggat
      541 acgtatatct acaatggcct gacatccaca ggagattttg gtatgacggc tgctgcagga
      601 ctttaccaat cagttgtcgg ctttgtcttg ttgatgatca caaatacgat cgcccgacgt
      661 tttgatagcg attctgcatt attctaagga ggaaaaaaat gaaaaaaaag aaagtggaga
      721 gagtccaagt ccgctctttc aataagacgg ctaatttttt ctctaatata atgattgcac
      781 tctttgctat ttcctgtatt ttgccattta tatttgttat cgccatctct tttaccgatg
      841 agtccatatt gtcgactcac gggtacagtt tttggccaga aaagttcagt atatttggtt
      901 atacgtactt gttcgaccag atgcaggata aaattttcca agcgcttttc gtcactgtac
      961 ttgttactgt tttaggtaca ctgatcaata gcacggctac ttctttatat gcttatgcga
     1021 tttcacggtc aaactttcca ttccgtcgtt tttttaccgt tttttgtttg attacgatgt
     1081 tgttctcacc agggatggtg gccaattatc ttgttatgac aaatcttctt cagttaaaag
     1141 atacgatttg ggcattgatc ttgccaatgg ctgtcagtcc cttcaatatt attgtgatgc
     1201 gcacattctt taaacgttcg gtctctgatt cgattattga atcggcacgg atcgatggag
     1261 ctagcgaact gcgaatattt gttcagatcg ttttaccgtt agctgttcca ggtattgcga
     1321 caatcagttt atttgccgca ttgggttact ggaatgactg gttcaatgct ttactataca
     1381 tccaagatga taagctagtt ccactacagt atcttttgat gaaaatccaa agtaatattc
     1441 aatatctgac acaaaattca ggagcaggaa gccaattgac aggcggatta gcctcagttc
     1501 caggagaatc tgccagaatg gcgatcgtag tcatctcgac gttgcctatt gcgatcagct
     1561 atccattttt ccagaagcat tttgtcaaag gtctaacaat cggcggagta aaagaataaa
     1621 gaaacaaaaa tataaatcat aaaattagga ggaagaaaaa tgaagttatg gaagaaacta
     1681 gcctttacag gtgtttcagc gttgttgttg ggaacattag cagcatgcgg aggttctggt
     1741 tctaaagacc aggcagaagc tagcaattcg gacactttgc agatgtatca aatcggagat
     1801 aaaccagata atttcgatca attgatggaa gtagcaaaca aacgaatcga aaaagagatc
     1861 ggtgtaaaag tcaatatcaa ttacattggc tggggcgatt acgaaaagaa aatgaacgtc
     1921 atcatttctt ccggggaaaa ttatgatatc gcctttgcga acaactatgt ttccaatgcc
     1981 caaaaaggag cctttgctga tttgactgaa ttagcgccta agtacgctga aaaagcttat
     2041 aatgatttag atgaagcata tattaaagga aatctggtca atggaaaatt atatgctttc
     2101 ccagtcaatg gaaatgtctt tgctcaacaa gtacttactt ttaacaaagc tttactagat
     2161 aaatacgatc tttcaattga tggaatccaa tcttacgctg atgcagaaaa agtcttgaag
     2221 gaattccatg aaaaagaacc aaacacggca gcttttgcca ttggacaagg cttcaaagtc
     2281 caagggaact ttgattatcc tttagggaat acgttaccat ttgctattga tctggatgga
     2341 gatgattcga aaatcatcaa ccaatacgat aatgaaacgt atatcggact gttacgtaca
     2401 atgcatagct ggtacaaaca aggactcatt ccaagtgacg ctgcaacgag caataaggat
     2461 tatccactag aaggaaatac ttggctaatg cgtggagaaa cacaaggacc gtatgattat
     2521 ggcgatacaa ttttgacaaa tgcagcaaaa caagaacttg tctcaaaagc aattactgtt
     2581 ccactaaaat ctacagctca agcacaaatg gcaaattttg tcgtatcaaa tacttcaaaa
     2641 aataaagaaa aatcagtaga acttctaggg ctgcttaaca gtgatccaga attattgaat
     2701 gggcttgttt gggggatcga gggagaagca tgggaaaaag tcgatggttc agatgataaa
     2761 atcaaattgc tagatggtta tcagccaaat acacatatgt ctgcttggaa tacaggaaac
     2821 aacaagattc tatatacaca agagtctatt acggatgaca tgattgccaa acgtgatcaa
     2881 tcgattgctg atgcagaaac ttcaccgatt ttaggattca gcttcaacac tgacagtgtc
     2941 aaaacagaat taagtaatat ttctaatgtc atgaatcaat atttagatgg attgaataca
     3001 gggactgttg atccagatga aacattaccg aaattgaaag acgctttgga caaggctggc
     3061 tatgataagg tattgaaaga aatgcaaaaa caatatgatg agttccgaca agaataataa
     3121 tgaataaaga caaaggggtt gtgacaaaag acgtgtcaca acctcttgtt ccgaataaac
     3181 ggtgttcaaa agccagtttc tcggtattaa gctaaatata tgtaaaaatg tggaaagcca
     3241 tattcacaaa atttttctta ctactcgaaa ctaagcggct atttcacaaa ctcttggtaa
     3301 acggtgttca atcagctaac cctcggtatt cgctcacaaa acgacaaaat atgaggaact
     3361 atttttccgt ttgtttctct aataatcagg tcaaggtgct gattgcccaa cctttttttg
     3421 tataatgaga aagtataaac ttaatgtttg gaggagaaga tatgttaggg aaggttttgg
     3481 aagagttatt tatccggatc tgggtcatta taaaactaac cttgtatttc tggatatata
     3541 catttgccgg cgggatcatc tttggattag gtgcggcatg gaaaacagtc aatgaactat
     3601 tttatttgta cggattcgag tataaagaaa tcacaataaa aagaggctgg aatatatata
     3661 aaagaaattt tttaagaggc aatctgctat ttagtttatt tttatctggt actgctttat
     3721 tgagttacaa tttatattta tctgttcaaa tcaaagggct cattttttta gctattgatt
     3781 ttattttact ttttgccttg ctctgcctat ttgctactta tcaatacagt atgattttag
     3841 atagtgaata tacgatctct gttcctaatc tgctaaagct aagttttatt tcggtttttt
     3901 ccagcttttc gtcttttcta aaaataatta ttggtagcgg tataatttta ggcgtgactt
     3961 ggcaattcaa aggattgatc ctgtttggtg tcattggtct tttgacggtt tggaatggta
     4021 cgatgacaac acattggcgt gaagaattag ataaacagtt ggaaagttat gaataagttt
     4081 cggattctat tttcaaaagg aaccttacta aatcgtctgt tgcggaaata cgcggttatc
     4141 atgatcggtg taacgatgac tgctaccgtt atttttagta tccatacttg ggaacaaaat
     4201 caaaaacagg cagaaaacat gacaagtgat gccgttcaaa gcacaagcag aatgctgaat
     4261 gataaaacaa ccttgagtag gatcatcaaa aaccagcttg tagggaattc cgaaaagatc
     4321 gaaaatgtga cgacctactt aacaaaaccc atagatcagt atctgatgta tgtatacgaa
     4381 cagcaaaaca gtaccgatga attagtttct ttcccaaatc aaatcaaaga tctctacgca
     4441 aattatgaag aactaagtgc gatatatatc gtgttgaatc aactaccgga gtattatgaa
     4501 tcttctagag aaaacaaagg tggggaaata aaaaagggga caccaaaatt actagacgcc
     4561 ttttatatca agttgcctat cacacaagga ggagctgagt caggaagtat ttttattggt
     4621 tttcaaaaga aagaactaga tatgatttta gaaaatctaa cgtcattcaa tggactctct
     4681 ttatacatga tttctggaac gacaaatcga ctatacacat tccatgataa taaaatagct
     4741 aaagaaacgt ttgaaagaca agaaatgatc atcacagaga gtctgaagaa aaattcacaa
     4801 ttgccgattg acgatctgga aaaaaacaat tttgttcaac accaagaact gtctgatgat
     4861 tatcgtatat tagctgtact agataaaaaa agtgtaaaaa tcgaaacggg aaaaaatctt
     4921 gccccgctga tcattggtat cattatttta gacaatatac tgcttatctt tctttataaa
     4981 acattcaaac gatattcaca gcaagtatct atcgtcatgc aggccatgga tcaaacagca
     5041 gcagggaatt tagagacgcg gatcgacata acaaacacgg aatatgaact gaaggaactt
     5101 tcaatcggta tcaacgaaat gttggatagc atcaatcagt ttgtagaaga catctacaaa
     5161 ttagaaatca aacagcaaga cgcacatatg cgggcgcttc aagcacagat caatccacac
     5221 tttttatata atacattaga atatatccga atgtatgcaa tcagcgaagg aagtgaagaa
     5281 ttagcagatg ttgtgtatgc gttttccgct ttgcttcgaa acaatacgaa tcaagagaaa
     5341 acgatcactt taaaagaaga actggacttt tgcgaaaaat atgtctattt gtatcaaatg
     5401 cgttatccta atcgagtagc ctatcatttt atgatcgatc ctgatttgga aaagatcgaa
     5461 gtacctaaat ttgtgatcca gccattagtt gaaaattatt ttaaacacgg tattgatttt
     5521 actcgattcg ataatgcgct aagtgtcaaa gtcttgcagg aaggcaaacg ggtaaggatc
     5581 atcatcaaag ataatggaaa agggatgact gaaaaacggc taaaacagat tgaagaaaaa
     5641 ctgtctcatc ctaaagtgga acttcacggt tctatcggtc tgcaaaacgt gaatgaacgt
     5701 ttacgtgcca gcttcggcag cagttattat atgtcgcttg agaataatga gacaggtgga
     5761 ctgaccgttt ctattacatt caaggaggga tgagcgtgta tcaagtgtta ttggtagatg
     5821 atgaatacat gattttgaat gggttaaaaa agataattga ttggcaaagt ttaggctttc
     5881 aaatagtagc aacagcagag aatgccaaag aaggactagc tgtacttgaa cagcgacaga
     5941 ttgatttagt agtaacggat gtgacaatgc cagaaataaa tggactggaa tttattgaag
     6001 cggctcaaaa agaacgtcat aattttgaat ttatgatttt atcaggatac caagaattcg
     6061 attatttgaa aggaggtatg caattaggcg ccgtgaatta tttgatgaaa cccgtgaata
     6121 agttcgaatt ggttgaaagc ctcaagaaaa tcaaaacacg gttagatcag caaaatgaac
     6181 aaaaaaatca acaagaaatc tatcaagaac tgttattcag tcaatggttg aatgaagaat
     6241 tggatgaaat aagtgaagaa gaaataatag gacaaataag tgagaagcag cataggatag
     6301 tattgattca actggcaaga actcatgata ctcttatcaa tagatggttg caagaacggc
     6361 aacaaatctt ttactatcaa cgaaattacg gagaccgtgt attgatcacg ttgttgttgg
     6421 aggcagatga agttgatggc ttctgttgtt ttgttcaaga aaactttcct gatcaggaat
     6481 ggctgatcag tatcggggaa gagacagatt ctttggacaa aatccctgaa acattccaac
     6541 aagcgaaaga caatctacag ttgcatcaat tttatggaga taaagacgag catgtttttt
     6601 acgcagtaca agcagaaaca aaagatcaaa ccatcgattt ttcaagtttc agacgcgtgc
     6661 tgcaaaataa acaattagaa gtcgctcaaa aaatgatcac tgattttttt gaacagtttc
     6721 agttagcagc catgatgccg gaagatatac gatattccag ttttttactg tttatggaga
     6781 ttcaacggga attgattgat ctggaagatg aggaatattt acaaggaatc gaaaaaatcc
     6841 atcaggcaaa aacggtgcaa gaacttcagc agctacttct gtcattcatc aaaaagcatc
     6901 agagacagaa aaagtacagt gacaatgtgg aaaaagtaat cgtaatcctg caccagcatt
     6961 atcaagaacc acttacactc aaagaagtgt cagaaaacct gcatttaaat gtcatgtatc
     7021 taggacagct gttcaaaaaa gaaacgaaaa aaagtttttc agcttatttg aaccatttac
     7081 gtatggaaaa agcgaaacaa ctgcttttgc acagtaatca aaacatcaat gagatagcaa
     7141 gtgagattgg ctacaacaac accacgtatt tctctaaatt attcaagaaa atcgtcggca
     7201 gatcccctaa agagtaccga gaaaacagtg gagaatcata aaagaaaaag ccaatgtaat
     7261 gaaataactt ataaaaaatt gtctagaaat ctaattttgg gctgttctta ttacttgagg
     7321 caaggcagct ttttcatgac ctctcgataa acggagttca aaagccagtt tctcggtatt
     7381 aagccaaatt tatgcaaaaa tgtggaaagc cattttcaca aaatttttct taatactcga
     7441 aactaaacag ctttttcacg acctctccac taaacggtgt tcaatcagct gaccctcggt
     7501 attagctcaa aaaatggcaa aatatgagga actattttgc catttgtctc tctaatactc
     7561 aggtcagaac gctgatttca caacctctca ataaacggtg ttcaaaagct tgcatctgcg
     7621 gtaataagac caaccaaacg aaaaatatga ggatctattt ttgcttggct gttcttatta
     7681 cttgatgcag gacagctttt tcacaacctc ttgttaatta tagtttgata aggaaaaagg
     7741 caaatctttt gcttgatatt tcttagggtg agggatgatc cctaattggt aagagatcgt
     7801 gccgcctttc agcaaatctt gatgggtaaa atataatttc gtatattctt tgtccatccg
     7861 tttgatttta tggataaatt gttgctgcgg ccgattaggc gttgcttcaa tcgtcaattc
     7921 ctttccattt gacaaaagca aaacagcctt gtcaattaat ggcataccga taacatattc
     7981 accggttcca ggcgtgacag gataaaatcc cagactattg aagatatacc aagcggccat
     8041 acttccattg tcctcatctc cagggtaacc cattggagaa tcgtcgaagg tctgagtcat
     8101 caactgttta agcagtggct gtgccatttc aggttgtcct atataactga acaagtaagg
     8161 atagtgaaaa cttggctggt ttgaaatagc taattgtcca aattctatcg cggccatctc
     8221 actcatttcg tggatctcaa aaccatatcc tcctgggtca aacgttgggg gctgattgca
     8281 cagctcaatg agtttttctt tatatctctc actgccacca tattttccga tcagtcctga
     8341 aaaatcgtga taaacagcaa agctgctttg ccaagcactt ccttctgcat aatcttgtcc
     8401 ccaacgatta gctgaaaatg gttctctgaa cttgccatct ttatcttttg aacgcataaa
     8461 tcctgactca tagtcaaaga tattttgata gttcaatgat tgctgctgat atttcttcgt
     8521 catttcctct tcttctagta ccttggcaac ttggctaata caaaaatcgc tataagcata
     8581 gtctaatgtg tgattgactg attcgtggta gtgattagga acatagccat atgtcaagta
     8641 atcttctgtt ccttggcgtc cataatttgg atgtttgctt tgaaccgtag cacctttttc
     8701 cattgccgtt agaaattcag gcatcaagtc tcgtcggata tcttttacag cagcatctgc
     8761 aatgaccgca tcgatcaatg tcccaggcat aagtccacgt tcatcaggag acagccattt
     8821 tggcaaatag ccactttctc tatagctgtt caaaaaacct tccagcattt cttcgtattt
     8881 ttctacagca atcaaagaat aaagaggata aaccgtttta tacgtatccc aaaacccatt
     8941 atttgtatag agaacaccag gttttataga tcgactagtc gtatcatagt ggattttttg
     9001 cccactagga tcaatttcat aaaatgtttg tggaaataaa aacacccgat ataaattatg
     9061 ataaaacgtc cgcaattgtt gttgatcatg gtgtgtgagc tgtatacgat caaaatagga
     9121 ctgccattgt tcttgactgt gagcaaggta agtctctttg ctttggtttt gttctagttc
     9181 caggttataa gctgcttgtt ccgagctgat aaaagatgtc ccaaatcgga tagtttgttt
     9241 ttttgtctgt tcaaaataca acgggatcga gtcgctattt ttgttggaaa cagctaactc
     9301 atcaattgtt aaaggatatt caaatctcaa tataaaataa aaagaaaaat caggatcttc
     9361 tgaaccagca aaatgtgtga tttttccttc cactgtgtaa ggatctataa caaagagctg
     9421 atggcgtccc ggaaaggata aaagtaaacc gctgtcattt ttgctgtaat caatagataa
     9481 gattccgcca tacatgctag gtatcaatgt agaacagatg ccataacgta attgacagat
     9541 cgacagatga gtcgggcaaa atatactttc ttctgggcgg taagaacttt gagcatggaa
     9601 aagggtgttc tcctgtagtt ttccattgat cggcgtaaac agcatatgac tgaaatcacc
     9661 catccaagga cttggttgat gggtaagacg gtagccttga aaaacgcggt catcaggatg
     9721 aaaccaccag ctgccgtttt gatccgtagt ttgcggtgca aaataattca tcccaaaagg
     9781 aacgccagta taaggctggc agttcccatt agagaaattt ggctgattcg ctgttgcatg
     9841 tctagtatct atttgaccga tattcatttt atttctcctt tgttgattgt cttttgatga
     9901 atgaaactgg aaatttacta tcggatggtc gatcttttgt ctctaaccaa gtgaccaaag
     9961 catctcccgc aagcttacct aactgttcaa aatcttgtgc gatggttgtc agtggcgggt
    10021 caacaagagc tgctgcttgg atatcatcaa atccgataat tgaaaaatct tcagggatat
    10081 gataaccatg ctgtctcaag agattcatgg tctggatagc gacaacatcg ttttcacaaa
    10141 caaaagcagt tacttcatac ttagtgatat aagaaagcaa ttcatctaat gtcgcgcttt
    10201 tgtcatccag tgaagtgtgg aaagtcaaac cagctttgtc caatgcttca aggtaaccaa
    10261 ggtaacgctg tcgaacggat tgtggatgag tctgcgtacc aaacaaataa gcgatttttg
    10321 aatgtccctg ttcgatcaaa taagaggcag caagcgcacc gccttggaaa ttatcggaaa
    10381 gaacggtagg aaaaggtaac tcatagattt ttttatctaa tatgacgaca gggaattctt
    10441 tcaatgaaag ctcaaaaagt aagtcaagat gttgttctgt atcaagagca taataaatca
    10501 atccgtcaaa ttcattgatt agctcttttg ctgttttatt ttgtagaaag tcaagcgtcg
    10561 tcatcatgac atcaatctgt ttatcttgca tcacagggtt caatcccttg ctaaaatcac
    10621 caacagacaa atcagcgaca tatggaagaa gaaacagaat ccgtcgtgtt tttgtaggag
    10681 aagcaagaat ctgttccgga gaagcggatt taacgaaact gcctttcccg cgaacgcgat
    10741 aaataagtcc taattgctct aattcagtca atgcacgttt ggaagtgatt ctgcttactt
    10801 tataggtacg tgaaagttct ttttcagttg gtagttggct gtctacaggt aattcaccag
    10861 ttaaaatagc cgtttgcaaa tcattaaata ttgtctggta caatggttga ttcatataaa
    10921 cacctcttaa tgatatatca tattcaaata ataccataat ggatgaaaaa gtaaataaaa
    10981 agatattttt tgaaagcgta gacataattg gctgtatgaa gtataatctt taaagatata
    11041 tcaaatagaa agaaggtttt attagtggct tataaagata taccaaaatc ggtgcagaaa
    11101 tttatggaag atatcacaca aaaatgtggg gatatccatg cggactgggc aaagaatttt
    11161 acagcagcat ttgccaatac tctgttgaca acagtcaaaa agcaaaatga cggaaccacc
    11221 tttttattga cgggagacat tccagcgatg tggttaagag actctactgc acaagttcgt
    11281 ccttatttag tgattgcaaa agaagatgag gaattagctg acatgatcag cgggttagtc
    11341 aaaaagcaat ttttttatat caatatcgat ccatatgcga atgccttcaa cgaagaagct
    11401 aacggtgctg gccaccaaac agatcataca aaaatgaacg attggatctg ggaaagaaaa
    11461 tatgaaattg attctttatg ctacccaata cagttggcct atcttcttta caaaaataca
    11521 ggaaaaactg atcaattcaa tcaatctttt attgaaggta tcaaaaaaat cttggacgtg
    11581 ttcaaaacag agcaagatca tgaacactct ccttatacat ttgtacgaga cacgacaaga
    11641 aaagaagata cgctgatcaa tgacgggcga ggtgcaaaaa cagcttttac aggaatgaca
    11701 tggtctggtt tccgtcctag cgatgatact tgccgttatg gatatctggt accatccaat
    11761 atgtttgcgg tggtcgtgtt aggttatgtg gaagaaattt ttcaaacgat actcgatgat
    11821 aaagaaattg tacagcaaac acagaaattg aagaaagaga tccaagaggg gattcagaat
    11881 cacggacgaa cggttaatca aaaagggcaa acgatttatg catatgaagt agatggaatg
    11941 ggaaacgcca cagtcatgga tgacagtaat gtgcccaatc tgatcgccgc accttacttg
    12001 ggatattgtt ctgaacaaga ggaagattat ctgcaaacaa gaagaacatt gttgagtaaa
    12061 gaaaatccat atttctatga aggaaagtac gcaaaaggaa ttggatcctc ccatacgcca
    12121 gaaaaatacg tttggccgat tgcattagct atggaaggaa tgacaacgtc agacaaatct
    12181 gaaaaagagc ggatattgaa tttacttgta gcaactgacg caggaacaca ccttatgcac
    12241 gaaggattcg atgtgaacaa tcctgaaaat tatacgagag aatggttttc ttgggcaaat
    12301 atgatgtttt gcgaattagt catggattac tttgatatta gagtagaaaa gtaggaggta
    12361 tcgtaaatga aaaagaaagt atatatcatt tctcatagcc actgggatcg tgaatggtac
    12421 atggcttacg aacagcatca catgcgactg attgaattga tagacgatct actagagcta
    12481 tttgaagttg atccgtcctt caacagtttt catttggatg ggcaaacgat tattttagat
    12541 gactatcttc aagtaaggcc agaaaaaaga caggcaatcc aacaagcaat caatgaagga
    12601 aaattgagaa ttggaccatt ttatatcttg caagatgatt ttcttatcag ctcggaatcg
    12661 aacgtgagaa atatgctgat tggcatggaa gaaagccgta aatggggaac ccctgtcatg
    12721 ttaggctatt ttcccgatac atttggaaat atgggacaga cgcctcaatt aatgaaacaa
    12781 gctggtattt cagcagcagc gtttggccgt ggtgtaaaac caattggctt tgataatcaa
    12841 gttttggaag cagaaaacta ttcttcccaa tactcagaga tgtggtggaa aggacctgat
    12901 caaacagcaa tctttggtct gctatttgcc aattggtata gcaacgggaa tgagattcca
    12961 gtagaaaaag aagcagctct tgccttttgg aagcaaaaat tagcggatgc agaacaatat
    13021 gcctctacga accacttatt gatgatgaat ggtgttgatc atcaaccggt acaaaaagat
    13081 atatcaaaag caatccatct cgcaaatgaa ctgtttccag attacgagtt tattcattct
    13141 aattttactg actatctgga agcagttcaa aaagatgtgc cagaagactt aggctcagta
    13201 gaaggagaat tgacaagtca agaaacggat ggctggtata ctttagccaa tacagcatct
    13261 gctcgggttt acttaaaaca atggaatacc aaggtccagc gacagctaga aaatattaca
    13321 gaaccacttg cgacgatggc ttatgaagtt agtggaaact atccccatga tcaattagat
    13381 tatgcttgga agacattaat gcaaaatcat cctcacgaca gtatttgcgg ttgttcagtc
    13441 gattctgtcc atcgggaaat gatacctcgt tttgaaaaag cagatgaagt tggaaagtat
    13501 cttgcacaag attccttaga acagctgacc gcagcaatcg atacgaccgg attcccaaaa
    13561 gacagctttc catttgtcat tgtaaatact gcaggaatgg ataaaactgg tgaagcagaa
    13621 ataaccatcg aattagaaag aaaaagattc gctgaaggaa tacctgaaca actatatcaa
    13681 gagttagaaa atttgcctaa acgtaagtat catgtagaaa caaaaagtgg ggccactata
    13741 ccagctattt tatctgaaga aactgtccaa tttggttatg atttgccgaa agaccgtttc
    13801 cgtgtgccat atatggcaag aatgatcaaa gtgactttac cattagaaaa catgccagca
    13861 ttttcttggg aaacattcgc gcttgtagaa ggggaagcga aagcagaaga aaaggaaaca
    13921 atgattcatc agagtggacg aatcatcgaa aacggccctc tacatttgac gattgaaaaa
    13981 aatggtacca ttacaatgga agatcgcaaa aacaaacgaa agttgaataa tctgcatatt
    14041 tttgaagata tcggcgatat aggaaatgag tatatcttta aacaaccttt ctgtgatcag
    14101 ccaatcctat ccagtaacaa agaaaactct gaagtaaaag tattagtaga tacaccggaa
    14161 atagcaaaaa taagtattct acaggaaatg gaaatccctg tttctgcaga cgagcgttta
    14221 gaaaaagaac agcagatggt tgtggaattt cgttatcgaa aagcagaacg atccaaagaa
    14281 aaacgaatcc ttcaaataaa aacaatcatg acaatcagaa aagatagtaa aaaaatcgac
    14341 tttgaaacga ctctcgacaa tcagatgaaa gatcatcgct tacgtgtctt gtttcctaca
    14401 aagttacatg tggaacatca cgaagcagat agtatttttg aagtggtgaa gcgcccaaac
    14461 catgtgtcta aaagttggga aaaccccacg aatccgcaac atcagcaagc atttgtcaat
    14521 attcatgatg aagagtacgg tgtaacagtc ggaaactttg gtttgaatga atatgaagta
    14581 acagaagacg gacaaatcgc agtgacgcta cttcgcagtg tgggagaact aggcgattgg
    14641 ggatattttc caacaccaga agcacagtgt ctaggtgagc atcggttcaa ttacagcatt
    14701 gaactacatg gaccagaaga aaaattctct acttatttgc atgcatacgc agcacagatt
    14761 cctttttcca ctcaacaaat caaacatcat gaaggaacat tgatttcaaa acaacaatat
    14821 ttaacgataa aaagtgaaac gtttgcaatt acagctttga aacgcagcaa gtttagtgat
    14881 aaagtagtag taagaggttt caatatgtct agtcatttag aaaaattgga aataactaaa
    14941 gacaatggaa aaactgtcat attgaatctg cttgaagaac caacgaagca agcggttgtc
    15001 ccaataatcc aaccttatga gattcggaca atcggattcg aggaggaaaa cgaatgtatg
    15061 gcggaataga agcaggcgga acaaaatttg tgtgtgcggt ttcagatggt ggagaaattt
    15121 tagaa
//
LOCUS       DAZKLM010000064        14668 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3764
            SAMEA115706913-rid22582563.denovo.066, whole genome shotgun
            sequence.
ACCESSION   DAZKLM010000064 DAZKLM010000000
VERSION     DAZKLM010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706913
            Sequence Read Archive: ERR13196647
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14668)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14668)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287823.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287823.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,004
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,849
            CDSs (with protein)               :: 2,849
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 41 of 96
            Pseudo Genes (incomplete)         :: 52 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 15 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14668
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706913-rid22582563.denovo.066"
                     /isolate="VRE3764"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(366..1484)
                     /locus_tag="ACG842_001907"
     CDS             complement(366..1484)
                     /locus_tag="ACG842_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0248441.1"
                     /translation="MAKKDKELLTQLTSEGAKGPFVTIMLNTHVAHQDVEKDQIKFKN
                     FAKEAKKRFEKKYSEHDWSPFQEKIENLLGDPSFWRSATTSVAVIITADQTYIHRLSI
                     PVDDQYYVSDLPYLLAIIKNGQFNYSYYLLGLNRDSMKLYEVSNKQVKEVSLPEGAPV
                     DVVTALGSELTGGGLNFSSNGGGGARDGVTFHGVNPKDEEVEIDWVNYYQAVDTYLKD
                     SFENPEQWPIYLFALPENQTMFKKIAKNPYYCADVAISASPAQLSFRDIEEGAKKLSS
                     ELAAQESKEYNKLLDKKFIDQLVDIVPAAKEGKVSHLFIATSNLADGFGEDPETEYDR
                     RQVLNALADDVIINGGKVFILDQKDAPDEKSLTAILRY"
     gene            1717..1839
                     /locus_tag="ACG842_001908"
     CDS             1717..1839
                     /locus_tag="ACG842_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0248442.1"
                     /translation="MHLIMIFSLLSIISLFAFSASRLLDAETQPIPVKVRKNDR"
     gene            1955..3262
                     /locus_tag="ACG842_001909"
     CDS             1955..3262
                     /locus_tag="ACG842_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA];
                     GO_process: GO:0030655 - beta-lactam antibiotic catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HFQ0248443.1"
                     /translation="MSIKKTRLVLLLASLILCLASFLPAVAVQAEDTFKVNAKAAFAV
                     DAQSGKILYDQDGEKTMGIASITKIIGLYIVLDQVKEGKLSWGDKVSISDYAENLSIT
                     PDLSNVPLHKENTYTVKELFDSAIIQSANASMVALAEKISGSEAKFTERMKEQLKDWG
                     IKDATIVNASGLNNSYLGENRPEGTGENDENQMSAQDVAIVARHLILDFPEILDVSST
                     TTQMFGENTQSPVEMVNWNWMLPGFVNYKEGVDGLKTGTTELAGACFVGTITKNDQRI
                     ITVVLNADGHAENPSARFNETDRLMDYCYDNWSEKELGKANASIPSLKTIDVKDGKES
                     TVPVVLKSPVKVWVRNDMDTGKLTITPTLDKKQVTDNEIQAPMDKGTKIGTATITLAD
                     DKLGYLEDNNAPSTEIITDKTVEKANIFVIGWRRVAEFFGNLF"
     gene            3381..4580
                     /locus_tag="ACG842_001910"
     CDS             3381..4580
                     /locus_tag="ACG842_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HFQ0248444.1"
                     /translation="MNLTRDLIFLVLYLIGVFSIVYWRKQKKDPSLFFYGSTWTFTVI
                     LTYFVVLEIFYSTFYFLIPLLFFSIFAFSYFTEKRRLLNGLLFNVLLISFGIYLFVLL
                     YETQNIFLGGLIALITIPLLLVLLFGIYGLIVFLFWNGVTVLRRESHSLANLLTLILA
                     IFLTLFLVFDFFLLKYLPQWINALFFCVPLILIYLFIVFYNFLTVSFLYQFNRPRYNQ
                     DFIVVLGAGLINGETVSPLLAKRINKAIAFYRAQSRATLNPPILLMSGGQGADEKVPE
                     AIAMKQYAMEQGIPERDILVETNSTTTLENMLYSKEIMDQQMKGPYRAIFSSNNYHIF
                     RAGLYARQAKLKANGIGAKTAFYYLPNAFLREYLAILMMNKKRHMIICGLLFAGSAFL
                     SLITLLF"
     gene            complement(4607..5875)
                     /locus_tag="ACG842_001911"
     CDS             complement(4607..5875)
                     /locus_tag="ACG842_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated chloride channel family protein"
                     /protein_id="HFQ0248445.1"
                     /translation="MEKQLPSPVRILVYMLKWLVITAILGIFMGSLSAFFLNSLTVVT
                     DIRLSHPWLLCLLPVSGALFAYLYAYHGGLSSRGNNLVIDQGNGGEEKIPLRLIPLTL
                     FGTITTHLFGGSVGREGTAVQMGGALADNTARLFRLDKSEREILIISGISAGFSSVFG
                     TPLAGTLFGLEVLAIGKVRTEAIFPSFFAALFANFMTESYGVSHLHYQMGVIPEWSML
                     LFFKVFVAGIAFGLIGWVFSRSIVFLKARYAQWIPHPVCRNTIGGAVVVAAALILQTQ
                     RYLGLSLPLLQESFNGQAHVYDFIGKLFFTVLSLGAGYQGGEVTPLFEIGATLGAALA
                     PLLHVSVPFLAGLGFIGVFSGATNTPIACFVMGIELFGSQAAVYFFMICLISFMCSGN
                     SGIYSAQQVAVKKGILFLPIVKRMKEKNKV"
     regulatory      complement(5930..5996)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01734"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Fluoride riboswitches increase expression of
                     downstream genes that help mitigate the toxic effects of
                     very high levels of fluoride; They are found in a wide
                     variety of bacteria and archaea; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="fluoride"
                     /db_xref="RFAM:RF01734"
     gene            complement(6038..6684)
                     /locus_tag="ACG842_001912"
                     /pseudo
     CDS             complement(6038..6684)
                     /locus_tag="ACG842_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728800.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FusB/FusC family EF-G-binding protein"
     gene            complement(7092..7595)
                     /locus_tag="ACG842_001913"
     CDS             complement(7092..7595)
                     /locus_tag="ACG842_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFQ0248446.1"
                     /translation="MTEKWREDEEYMSYVEDLLETEAVKKLANYTQHVHSTRLEHSIS
                     VSYYSYLLAKKWGGNAKATARAGLLHDLFYYDWRTTKFDEGTHAYIHPRIAVKNAEKI
                     TELSDLERDIILKHMWGATIAPPKYKEGYIVTFVDKYCAVKEAAQPMSASMRKRWQRY
                     FGKESSI"
     gene            complement(7718..8482)
                     /locus_tag="ACG842_001914"
     CDS             complement(7718..8482)
                     /locus_tag="ACG842_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HFQ0248447.1"
                     /translation="MKEIQSAKNPRIKELKKLHKKKYREEKEEYLLEGFHLIEEAASS
                     DAEIKELFVTQRGMKEWGDWLAANKEISTFLVSDEVMDAVSDLPTPQGILAVMALTAE
                     GTPDFSGGWLLLDNVQDPGNVGTMIRTADAFDMAGVLLGKGTADIYSTKVLRSMQGSN
                     YHLPVLQKDLTEVISLMKDKGFTVYGTELNEEAHALDKVEKSDNFAIIMGNEGQGVSQ
                     EILSLTDQNVYIPMKGNAESLNVGVAAGIVLYHFSL"
     gene            8590..8865
                     /locus_tag="ACG842_001915"
     CDS             8590..8865
                     /locus_tag="ACG842_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HFQ0248448.1"
                     /translation="MRKVKMNVQGRVQGVGFRYMTKMVADQLGVTGTVKNEDDGSVTI
                     EAIGNDDIIQKFIEEVKKSPSPSGRVQYVDVQEDPMIEERKKFDVVG"
     gene            9303..10253
                     /gene="yidC"
                     /locus_tag="ACG842_001916"
     CDS             9303..10253
                     /gene="yidC"
                     /locus_tag="ACG842_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0051205 - protein insertion into membrane
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HFQ0248449.1"
                     /translation="MNKWKNWLLGSGLVSILLFLSGCVRMDSNGNPDTSGIVYRVLVH
                     PMGQAITYLVENFNWSYGWAVIAMTVIVRIIILPLGISQSKKTMIQSEKMQALKPQVE
                     AAQQKLKAATTREEQMAAQAEMQQVYRENGLSMTGGIGCLPLLIQMPIFSALYFTARY
                     TEGIRESSFYGIDLGSPSMVLVAIAGVAYLLQGYISTIGIPEEQKKTMRTMLIVSPAM
                     IVFMSISAPAGVTLYWVVGGVFSCLQTFITNVMMKPRLKAQVAEELKQNPPKQVVTPR
                     IDVTESAKPADSKQATKQIKQPKNSTNNKGRNAGKQQKRK"
     gene            complement(10432..10611)
                     /locus_tag="ACG842_001917"
                     /pseudo
     CDS             complement(10432..10611)
                     /locus_tag="ACG842_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308674.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            11437..12396
                     /locus_tag="ACG842_001918"
     CDS             11437..12396
                     /locus_tag="ACG842_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30-like element IS6770 family transposase"
                     /protein_id="HFQ0248450.1"
                     /translation="MTYKHLTIDELTMIESYYLQHNKPVEIANRMGRAIQTIYNVVNK
                     FKQGKTALDYWHQYKENKKKCGRKVIQLPAHEVDYIKEKVTLGWTPDVIIGRKERSVS
                     CGMRTLYRLFSKGIFDIDTLPMKGKRKPNGHQEKRGKQQYQRSIHDRPDNYPDFNSEF
                     GHLEGDTIVGIHHKSAVITLVERLSKVIITIKPNGRKALDIETALNQWFSRFPKNFFK
                     SITFDCGKEFSNWKAISNQHDIDIYFADPGTPSQRPLNENSNGILRRNGLPKSIDFRE
                     VNQTFISSVSNQRNHIPRKSLNYRTPIEIFLSYVQEAFYSSLI"
     gene            complement(13262..13687)
                     /locus_tag="ACG842_001919"
     CDS             complement(13262..13687)
                     /locus_tag="ACG842_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transferase"
                     /protein_id="HFQ0248451.1"
                     /translation="MHRKVRITRKSRGKIEIVLQSGKIVYLGFPVLNTAGNEETIISI
                     EGILRIQGPAFIAQGAHIDIRKNAVLDIGSKFNSTGKMSIICFKEIQFKDGCTVSWDT
                     LFMDTDSHTIFELNTHRILNKNNKIVIGKHCWIGAKSVI"
     gene            complement(13789..14406)
                     /locus_tag="ACG842_001920"
     CDS             complement(13789..14406)
                     /locus_tag="ACG842_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HFQ0248452.1"
                     /translation="MIIVIAEIITDILYKLYEPFGFAVLLTILGLVYFMYATDGIAAG
                     EGYKRILMELYLHLKKDRKYRKVCALIFYTAMILFTTLINRTIWLNPLSDVMGDWWIY
                     KYNTTTGTKELSTACLENIILFIPYVYMVMSIKYENIKKPKIWSSIVYGGKIAFYFSA
                     IIEFLQLFLRLGTFQISDFVYNTLGGILGVILYKLILITKRDGER"
BASE COUNT         4526 a         3013 c         2431 g         4698 t
ORIGIN      
        1 tttaaacggt gttccagaag tagcttcttc ggaaataagc cgaaatttca caaaaatttg
       61 agaaataatt tttgtcaatc tcttcttatt tcttgaagct gaccacttct gtcacaacct
      121 cttccttaaa cggtgttcaa aagcttgcat ctgcggtaat aagcccacca aacaaaaaat
      181 atgaagagct atttttgctt gctgttctta ttacttgatg cagaacagct ttttcacaac
      241 ctctcataca cggtgtttaa tcagctgacc ttcggtatta gctcaaaaaa cggaaaaata
      301 tgtgaaacta tttttccgtt tgtttctcta atactcaggt caaaacactg atttcacaac
      361 ctcttttaat aacgaagaat cgctgtcaga ctcttttcat ctggcgcatc tttttgatcc
      421 aaaataaaca ctttcccgcc attaatgatc acatcatctg ctaatgcatt cagcacttga
      481 cgccgatcat attctgtttc aggatcttca ccgaaaccat cagctaagtt tgacgtagcg
      541 ataaataaat gtgacacttt tccttctttc gcagctggaa cgatatcaac taactgatcg
      601 atgaatttct tatctaagag tttattgtat tcttttgatt cttgagctgc gagttcactt
      661 gacagcttct tcgccccttc ttcgatatct ctaaaggata gctgcgcagg agaagcagaa
      721 atcgcaacgt ctgcacaata atagggattt ttagcaattt ttttgaacat cgtctgattt
      781 tctggtaatg caaataaata aatcggccat tgttcaggat tttcgaaaga atccttcaag
      841 tacgtatcaa cagcttggta atagttgacc caatcgatct ctacttcttc atcttttgga
      901 tttacgccat ggaaagttac tccatctcgg gctccgcctc ctccattaga agagaagttc
      961 aaaccgccac ctgtcagttc agatcccaat gcagtaacga catcaaccgg tgccccttct
     1021 ggtagagata cttcttttac ttgtttgttg ctgacctcat acaatttcat ggagtcgcgg
     1081 ttcaatccca ataaataata agaatagttg aactgcccat ttttgataat agccagtaaa
     1141 taaggaagat cacttacata gtattgatcg tctaccggga tactcaaacg atggatatag
     1201 gtctggtcag cagttataat gaccgctaca ctcgtagttg cactacgcca aaacgaagga
     1261 tctcctaaca aattttcaat tttttcttgg aacggggacc agtcatgttc ggaatatttt
     1321 ttctcaaaac gtttctttgc ttctttcgca aaatttttga atttgatctg atctttttcc
     1381 acatcttgat gggcgacatg cgtattcagc atgatcgtga cgaatggtcc tttagcgcct
     1441 tctgaagtca attgtgtcaa taattcttta tccttttttg ccataaacgt acccctccta
     1501 aaaaaatcat tcctttgttt ctatctaaag tctaccacaa cactattttt ccatacaata
     1561 aaacgtatct aaaaaaataa aacggtttct acggttttaa atttagctat ttttttaagt
     1621 tatcttcata atttcctcaa ttatttagcc ccagtcttca actattcttc atgaaatgct
     1681 gataaactaa gaaaaagaat aaaagaggtg gatcttatgc atttgattat gattttcagt
     1741 ttattaagta ttatcagttt atttgctttc agcgcttccc gcttattaga tgcggaaaca
     1801 cagcctattc ctgtaaaagt acgtaaaaac gaccgctaaa cttccataac gcgcgttctt
     1861 atcctaaagt tttttcagaa acattgaaac tctctcccct ctctgataaa attttaattt
     1921 gtagcgaaac aaattaatta caaggagatc ggatatgtcc ataaaaaaaa cacgtctagt
     1981 cctattattg gctagtctca ttctttgcct tgcttctttt ctaccagctg ttgctgtgca
     2041 agctgaagat acgttcaaag taaatgcaaa agcagctttt gctgtagatg cgcaaagcgg
     2101 taagattctt tatgatcaag atggagaaaa aaccatgggg atcgcttcaa tcacaaaaat
     2161 catcggtctt tacatcgtcc ttgatcaagt aaaagaagga aaactgtcat ggggtgataa
     2221 agtttcgatt tcagattatg cagaaaattt gagtatcacc ccagatttat cgaatgttcc
     2281 tttgcataaa gaaaacacct acactgtcaa agaattattc gactctgcaa tcatccagtc
     2341 agccaatgct tcaatggtgg ccttggcaga aaaaatctct ggcagcgaag ctaaattcac
     2401 tgaacgaatg aaagaacaat tgaaagattg gggaatcaaa gatgcaacaa tcgttaatgc
     2461 atcaggattg aacaactctt atcttggcga aaaccgacca gaaggcactg gagaaaacga
     2521 cgaaaatcaa atgtctgctc aagatgtggc catcgttgcc cgccatttga tcttggattt
     2581 cccagaaata ctggatgtat caagtactac gacccaaatg tttggcgaaa atacgcaatc
     2641 accagtggaa atggtcaact ggaactggat gctcccagga ttcgttaatt acaaggaagg
     2701 cgtggacgga ttaaaaactg gtacgactga gcttgcaggt gcttgtttcg ttggtacgat
     2761 cactaaaaac gatcaacgga tcatcactgt cgtattgaat gcggacggcc atgcggaaaa
     2821 cccgagtgcc cgcttcaatg aaacagaccg tttgatggac tactgctatg acaattggtc
     2881 agaaaaagag ctcggaaaag ctaacgcaag tattcctagt ctaaaaacca ttgatgtaaa
     2941 agacggaaaa gaatcaacag ttcctgttgt gcttaaaagc cctgtaaaag tatgggtacg
     3001 aaacgatatg gatactggca aattaacgat cacgccaaca cttgacaaga agcaagtcac
     3061 tgacaatgag atccaagcac ctatggataa aggtacaaaa atcggtaccg ctacgatcac
     3121 cttggcagac gataaattag gctatttaga agacaacaat gcaccatcaa cagagatcat
     3181 tacggataaa acagtggaaa aagcgaatat attcgtgatt ggctggcgac gcgtcgcaga
     3241 atttttcggc aacttgtttt aattgaacaa taagaaaaac atctttccat cggaaagatg
     3301 tttttttgct ttttctagtt taaataacag cttttttcat ctattttcgt tatactgtat
     3361 aagaaaaagg agttgaattt atgaatctaa ccagagatct gatcttttta gttctctatc
     3421 ttattggggt attctctatc gtttattggc gaaaacagaa gaaggatcct tccttgttct
     3481 tttacggcag tacgtggaca tttacggtaa tcttaactta ttttgtcgtg ttggaaattt
     3541 tttattcgac cttttatttt ttgattccat tgttgttctt tagtatcttt gctttttctt
     3601 atttcacaga aaaacgaagg ctactgaatg gtcttttatt caatgtttta ctcatcagtt
     3661 tcggcatcta cttgtttgtc ttgctgtatg aaacgcaaaa tatttttctt ggcggactga
     3721 tcgccttgat cacgattcca ttgctacttg tccttttatt tggtatttat ggattgattg
     3781 tttttctttt ttggaacggg gtcacagttt tacgtagaga atcgcattca ttagctaatc
     3841 ttttaacgtt gatactagcc atttttctta cactgtttct tgtttttgac tttttcttgt
     3901 taaaatattt gccgcaatgg ataaatgctt tgtttttctg cgttcccctg atcttgatct
     3961 atctatttat cgtattctac aactttttga ctgtctcatt cttgtatcag ttcaaccggc
     4021 ctcgatacaa tcaagacttc atcgttgtgt taggcgctgg attgatcaat ggagaaacag
     4081 tttctcctct tctagccaag cgaatcaata aggcaattgc tttttatcgg gctcaaagcc
     4141 gtgctacatt aaatccgccc atcttattga tgtctggcgg gcaaggagca gacgaaaaag
     4201 taccagaggc cattgcgatg aaacaatatg ccatggaaca aggaatccct gaaagagaca
     4261 ttcttgtcga aaccaattcg acaaccactt tggaaaacat gctctattca aaggaaatca
     4321 tggatcagca aatgaaaggg ccataccgcg cgatctttag ttccaacaat taccacattt
     4381 ttagagccgg tctttatgcg cggcaagcca aattgaaagc aaatggtatt ggtgcaaaaa
     4441 cagctttcta ctacttgcct aatgcgtttt taagagaata cctcgccatc ttgatgatga
     4501 acaaaaaaag acatatgatc atttgcggat tgcttttcgc aggctccgca ttcttatcgc
     4561 tgattacttt acttttctaa aaaaacaagg tgtgacaaaa catatgtcac accttgtttt
     4621 tttctttcat tctcttaact attggcaaaa agagtatccc ttttttgacc gctacttgct
     4681 gagcagaata aataccgctg ttgcctgaac acataaagct gatcaagcag atcataaaga
     4741 agtatactgc cgcttgtgaa ccgaacaatt cgatccccat cacaaagcag gcaatcggtg
     4801 tatttgtcgc accagaaaat actccgataa agcccaatcc tgcaagaaaa ggaacagaaa
     4861 catggagcaa aggcgctagt gctgcaccaa gcgttgcacc aatttcaaac aatggtgtca
     4921 cttctccccc ttgatatccc gcgcctaaag acaaaactgt gaagaataac ttcccaataa
     4981 aatcatagac atgtgcctgt ccattaaatg attcttgaag taacggaaga ctcaatccga
     5041 gataacgttg tgtttgcaaa atcaatgctg ctgccacaac tacagctcca cctatcgtat
     5101 ttcgacaaac aggatgagga atccattgtg catatcgcgc ttttaaaaag acgatcgaac
     5161 ggctgaatac ccaaccaatc aaaccaaaag caatcccagc aacaaaaact ttgaagaaca
     5221 gcaacatact ccattcagga atcacaccca tttggtaatg tagatgagaa acaccatagc
     5281 tttctgtcat gaaatttgca aacagtgcag caaagaagct agggaagatc gcttctgttc
     5341 ggactttccc gattgccaac acttctaatc caaataatgt tccagctaaa ggtgtgccaa
     5401 atacagaact aaaacccgca ctgattccac tgataatcaa gatttcccgt tctgatttat
     5461 ccaatcggaa caaacgtgcc gtattatctg ctaacgctcc ccccatttgg actgctgttc
     5521 cttcacggcc aacagatccc ccaaataaat gggtagttat ggttccaaac agtgtaagtg
     5581 gaataagccg caacgggatt ttttcttctc caccatttcc ttgatcgatc actaagttgt
     5641 ttccacgact agaaagtccg ccgtgatacg catagagata agcaaaaagc gcaccactaa
     5701 ctggtagtag acaaaggagc caaggatgtg atagtcggat atctgtcaca actgtcaggc
     5761 tatttaagaa aaaagcagac aagctcccca tgaatatacc gagaatcgct gtgatcacca
     5821 gccacttcag catatacact aaaatgcgta ctggactcgg taattgtttt tccataaaaa
     5881 attcctcctc aataaaaaaa cgcctactaa agaaaggggc tgtccccaga ctttagtagg
     5941 cgtcattagc tatctttcgt tagcatttaa aggcgaacac catcacctat tcacttccat
     6001 taaaagatag catatttttc tgtaaaaaac aataaaatta tcgttctttc tttacacgtt
     6061 cagcaaattc atagaaagga gccaatttcg ttaactgttg attacattgg tcactatcat
     6121 gacaaatata attccccttt ttcgtatacg tgccatcaga acctgctttc gttgtagaaa
     6181 gaaaaagcga aacattcgat gttttctgac agattgcaca gattccctta tttatcgttg
     6241 gtgataaagt accgctaatc ccttttaatt tttcatcttc gtaatagacc atgaattttt
     6301 tctgcgtacc tggatcattc cagccgatgt acgtatgttc ttgcaagtcg atttcattcc
     6361 aagctggttg tttgagtttt ttcacttttc ggaataattt ctcgatttgt ggtttgctag
     6421 gctgcataaa cggctgaacg atttgcttca gctcctccaa tgctctttct gcttgcgctt
     6481 ttgtcagttt tacgtctaaa actgtcgtta aaaatgtatc gattctggca gttcgtgatc
     6541 taatatgtct cgaatctttt cagctgctag tgcctgaact gtttgtaccg tttttggatc
     6601 attgacagat gagtacgcat ttaataactg ttctacttct cttattataa aaaaatattg
     6661 gtatggtttt attgtttgtt ccatgatttt aaagtccttt cttctaacca aagtgcatac
     6721 tttgtagaaa aaagtgatct tctgcaaagt atgtgttacc tgaagatccc ggactctttt
     6781 aaataagata cgttttgcat cttatcatca ccccctttaa tgcttattat tctatgtaca
     6841 tgattcattc gataaataaa agtaaaccgt atcttgaaca gatagtcaac tgcaaacaga
     6901 aaaaaggact gtgacaaaag tcgtgtcaaa gtcccttgtt tcgaatgaat ggtgttcaaa
     6961 agctggcatc tgcggtaata agccgaaact tcacaaaaat ttgagaagca attttcgtaa
     7021 attccttctt atttcttgaa gctgaccact tctgtcacaa cctcaatatg gtctattatt
     7081 ggttttatct attaaatcga ggattctttc ccaaagtaac gttgccatct cttacgcata
     7141 cttgctgaca taggctgagc ggcttctttg actgcgcagt atttgtctac gaatgttacg
     7201 atatatcctt ctttgtactt aggcggtgca atcgttgcgc cccacatgtg cttcaaaatg
     7261 atatctcgct caagatccga caattcggtg atcttttctg cattctttac agcaattctt
     7321 ggatggatat acgcatgtgt gccttcatca aatttcgtcg tacgccaatc atagtagaac
     7381 agatcatgca aaagtccagc acgcgctgtt gctttagcat tgccacccca tttttttgcg
     7441 agcagataac tatagtaaga aacactgatg gaatgctcca aacgagtgga atgaacatgc
     7501 tgtgtgtaat tcgctaattt tttcactgct tctgtttcta aaagatcttc tacataagac
     7561 atatattctt cgtcttcacg ccatttttct gtcataatta tcgatctgcc acctttttga
     7621 atttcatttt cttctgaaat tgatctaagt ataccaccag tgttcggatt attctagtcg
     7681 ccttggataa aatagccaaa gcttaatagt tccttaatca taaagaaaaa tgataaagaa
     7741 caatccctgc cgcaacccca acattcaacg attccgcatt tcctttcatc gggatatata
     7801 cgttctgatc agtcaaagac aagatttcct gacttacccc ctgaccttcg ttccccatga
     7861 ttattgcgaa gttatctgat ttttctactt tgtctaatgc gtgtgcttct tcatttaatt
     7921 ctgtgccata cactgtaaac cctttgtctt tcattaaaga aatcacttct gtcaaatctt
     7981 tttgcaacac aggcaaatga taattgcttc cctgcatgct gcgcagcacc tttgtactat
     8041 aaatatctgc cgttcctttg cctaataaaa caccagccat atcaaatgca tctgccgtac
     8101 ggatcatcgt ccctacattt ccaggatctt gtacattatc taacaacagc catcctcctg
     8161 aaaaatctgg cgtgccttca gccgttaacg ccatcactgc taagattcct tgtggtgtag
     8221 gtagatccga tacagcatcc atgacctcat ccgaaactag aaaagtagaa atctctttgt
     8281 ttgcggccag ccagtctccc cattctttca tgcctcgctg tgtcacaaaa agttctttta
     8341 tttctgcatc agacgacgca gcttcttcaa tcagatggaa cccttccaaa agatattctt
     8401 ccttttcttc ccgatatttc tttttatgta gtttttttaa ttctttgatt cttggatttt
     8461 ttgctgattg gatctctttc acattcctca cctccccatg attatatcat ggaataggtg
     8521 cttgagcttg tttcatccta aaaaaatcgg tatcattaat ttataaaata ggaaagaaag
     8581 gtgacgaaca tgcgcaaagt taaaatgaac gtccaaggac gcgtccaagg cgtaggattt
     8641 cgctacatga ccaagatggt cgctgatcaa ctaggcgtga ctggaacggt aaaaaacgaa
     8701 gatgacggct cagtcacgat cgaagcaatc ggcaatgacg acatcatcca gaaatttatt
     8761 gaagaagtca agaaatctcc gagcccaagt ggacgagtgc agtatgtgga tgtccaagaa
     8821 gacccgatga ttgaggaacg aaagaaattt gatgtggtgg gataaggttg tgaaaaagct
     8881 ggtctgcatc aagcattaag gacaggttgt gaaaaatagc tctccatatt tttcaacagg
     8941 ctggacttaa tgccgtagat gcaagctttt gaacaccgtc gataaggtta ttcttgaggc
     9001 gctttgcctc aaaaattaag gacagcagtg aaaaagctga tctatcactt gcagtgagtt
     9061 catttgactc cgctttttcg ggcagaaaaa aagtttttag tttattaaag taagaaagaa
     9121 acacttggaa tcattctact ttttcctagt atttctttct tgattttttg tctttttttc
     9181 aaaatttttc gctacgtaaa agaacgaata tttatatgaa atttctatga agttgattga
     9241 attccatgcg cttttttagt atagttaagt ttgtgtaaaa attatagaag aggaagtatt
     9301 ctatgaataa atggaaaaac tggttattgg gatccggact tgtcagtatc ctgctctttt
     9361 tatctggctg tgtgcggatg gattcaaacg gaaaccctga cacttcggga atcgtttatc
     9421 gagtactcgt ccatcctatg ggccaagcga ttacatatct agttgaaaat ttcaattggt
     9481 cttatggctg ggcagttatt gcaatgactg tgatcgtccg tatcatcatc ttgccactag
     9541 gtattagtca atcgaaaaaa acaatgatcc aatctgaaaa aatgcaagcg ttaaaaccac
     9601 aagttgaagc agcccaacaa aaattgaaag ctgcaacaac acgtgaagaa caaatggcag
     9661 cacaagcaga aatgcagcaa gtataccgcg aaaacggatt gagtatgacc ggcggaatcg
     9721 gctgtctgcc tctattgatc caaatgccga tcttctctgc cttgtacttt acggcacgtt
     9781 atacagaagg tatccgggaa tcatctttct acggcatcga tttaggctca ccaagtatgg
     9841 tacttgttgc cattgcaggg gtcgcttatc tgcttcaagg atacatctca acgatcggta
     9901 ttcctgaaga acagaaaaag acgatgcgta cgatgctgat cgtctctcct gctatgatcg
     9961 tcttcatgtc gatcagtgcg ccagccggtg ttacacttta ctgggtagtc ggtggtgtgt
    10021 tcagctgtct gcaaacattc atcacaaacg ttatgatgaa accacgtttg aaagcacaag
    10081 tggcagaaga gttgaaacaa aacccaccaa aacaagtcgt cactccaaga attgatgtga
    10141 cagaatcagc taaaccagct gacagcaaac aagcaaccaa acaaatcaag caacccaaaa
    10201 acagtacaaa caataaaggc cgcaacgcag gaaagcagca gaagcggaag tagatagtaa
    10261 ataagagtgt aataaaaatt cattaacagt tttagcaatt aaaaatatcg accaagattt
    10321 ttgaaaaaga aatctggtcg atatttttat ttaagtgaat gtttaatttg aatcatcgat
    10381 tgatcgctat ctaattgcag cagcatcttt ttatgaagaa acaacttttt cttatctatc
    10441 aacgtagaca ggtgttgctg tttcttctaa tagtgtactt aattccttaa tattttgttt
    10501 tccttcttct aacatccaac tactcgcttt ttcttcgcct tcggtaacaa aaggcgccac
    10561 tcccttcgcc catgttgctc gcccacataa gacaccattc tccataaagg acaacgcgga
    10621 gataccaccc atctaaataa catccataaa cctttctagt agaagaatag tgatagtatc
    10681 catccctttt tctactggga atacaattac acgattcaaa gcttgataca caacatttcc
    10741 cttcttataa accgctattg taaccgaatt gttttgaatc acctttttgg gatagaaact
    10801 ggctcctttc cgcaacttct taaatgttgg tttcttttat actttctttg tagcaactac
    10861 aaatttcttc aaatctctaa atgtttgcat aacaaccgcc tttctgactt ttactcagga
    10921 tggtatttaa aaatcagcaa aataaggctt ctctccatct tttttatttc ctagctgctt
    10981 taaagacact cttgctgttt tttaatctct atcaaggggg ctctgaatct attttgatga
    11041 acttatctta ttaatttctc aaatgagctg tcagacacaa ctgtattttt tcaatctcat
    11101 accggacaac tatgccagct taaaaaaatt tcctctggca aaacttcttt attgaaaaat
    11161 gacaatgaaa aaagaccatt ctttttgttg aatgctcatt gtgtaaacat atttcctgcg
    11221 actgctcttc gaaccattct cagtgcaatc cctaatttat ctattaaatc ttcttttagt
    11281 tcctcatctt tcatattcaa aatttccttt gtatgttaca caagtttgtt ctctaaataa
    11341 cgcgaattgt aaaattaagt tagaaaaata aaaaggcatt tatggtacac tcaaattgta
    11401 tttccgacga aagaaaacaa aggagtgtaa ccataaatga cctacaaaca tcttaccata
    11461 gacgaactga caatgataga atcatattat cttcaacata ataagccggt tgaaatcgct
    11521 aaccgaatgg gacgtgctat acaaactatt tataatgtag tcaataagtt caagcaaggc
    11581 aagactgctc ttgattattg gcaccagtat aaagaaaata agaaaaaatg tggtagaaaa
    11641 gtcattcaat tacctgctca tgaagtagat tacattaaag agaaagtcac tcttggttgg
    11701 acgcctgacg tcattatcgg gcgaaaagaa aggtctgttt catgcggtat gagaacactt
    11761 tatcgtttat tttctaaagg aatatttgat attgacacac taccgatgaa aggtaaaaga
    11821 aaacccaatg gccatcagga aaaacgggga aaacaacagt atcagcgctc aatccatgat
    11881 cgacctgata attatcctga tttcaattct gagtttggtc accttgaagg tgatacgatc
    11941 gttggcattc atcataaaag tgccgtcatt actttagttg aaagattatc taaagtcatt
    12001 atcacgatta aacccaacgg ccgtaaggca ttagatattg aaactgccct taatcaatgg
    12061 ttttctcgct tccctaaaaa cttctttaaa tctattacgt ttgactgtgg aaaagaattt
    12121 tctaactgga aagccattag taaccaacat gatattgata tatattttgc ggaccctgga
    12181 acaccttctc aacgcccatt aaacgaaaat tctaacggga ttctgcgtcg taatggactg
    12241 ccgaaatcaa tagattttag agaagtgaat cagacattta tttccagtgt cagcaatcaa
    12301 cgcaatcata ttccaagaaa atcattgaat tacagaacac caatcgaaat atttttgagc
    12361 tatgtacaag aagcatttta ttctagctta atttgacaaa tcatataatt taaaattaac
    12421 attgattctc ctatgaaaat atcttttacc tttctcctgt gaaaaggaaa tttccttttt
    12481 tatagtgtta ttgatataat cagaatattc atcaaactct gtatctggag ttaaaaatct
    12541 acagtaacat ctataagctt tgctagtaat cttcttgggt tctctctcca ttgtcaagca
    12601 acatcaaaac acctacttac tattcgattc ttttctgcca aaaacgagtc agcatcactc
    12661 acaacttatg tgttgctgac tccgactagt cctttgttca atatcaactt attaaacaga
    12721 tataatattt ttctttattt cgtatactcc aagacttcca tctttctttc atcttatctc
    12781 tgaaaaaaac acagcaaagt gcccttaata aatatacatt agaatttgca aattttttca
    12841 tcttaagata tatcctattc atattcttta caatcctaat gggtgattac aaatctattt
    12901 agttcaagtt ctacattata aacacctttc ttgatagtat catctgctgc acaagcgatg
    12961 tcgatccact tattagggga tttacgtgct caaaagtggc tattcctttt gggaagtcca
    13021 tccataataa tggtccatgg atttttttag ggtcttgtcg ttcaaatttc tcaaaaactt
    13081 tattatactt ccatagtttt ataattattt ggtttcactt tggctcgttc aaacaccaac
    13141 ttgtatttct tttcaacgtt cgaactggag ccccccctat aatctcatta ttattttcat
    13201 gactttttac taccgtggaa ttacaagcaa caacaatatt atctgctaaa tgaacacctt
    13261 tctatatcac gctttttgct ccaatccaac aatgtttacc tataactatt ttattatttt
    13321 tattcaaaat cctatgagta ttcaattcaa aaatcgtatg tgaatcggta tccataaata
    13381 atgtatccca cgatactgta cagccatctt tgaattgaat ttccttaaag caaattatac
    13441 tcatttttcc tgtcgaatta aactttgacc caatatctaa aactgcattt ttccttatgt
    13501 caatatgagc cccttgtgcg ataaacgctg gcccttgtat tcttagtatt ccttcaatac
    13561 ttataatcgt ttcttcattt cccgcagtat ttaatactgg aaagcccaaa taaactattt
    13621 ttccagattg taatactatc tcaattttcc ctctcgattt gcgtgtaatc cgtactttac
    13681 gatgcaccaa aataggtaat ctccacgcat ctgaaatatg aagaaattta taattgaagt
    13741 atatcgtttt aggtaaattt aatatattct ttattatcaa tccgagtctc atctttcacc
    13801 atctcttttt gtaatcaaaa ttagtttata aaggattaca cccaatatac cgcccaacgt
    13861 attatataca aaatccgaaa tttgaaacgt tcctagcctc aaaaaaagtt gtaaaaattc
    13921 aattattgct gaaaaataaa atgcaatttt tcctccatat actattgagg accatatttt
    13981 gggtttcttt atattttcat atttaataga cattaccata taaacatatg gaataaaaag
    14041 aataatattt tctaaacacg ctgtagataa ctcttttgtt ccagttgttg tattatactt
    14101 ataaatccac cagtctccca taacatcaga taaaggattt aaccaaattg ttcgattgat
    14161 taatgttgta aataaaatca tcgcagtata gaatatcaaa gcacaaactt ttctatattt
    14221 tctatccttc tttaaatgaa gataaagttc cattagtata cgtttatagc cctcacctgc
    14281 agcaatgcca tctgttgcat acataaaata gaccagtcct aaaattgtca ataaaacagc
    14341 aaacccaaag ggttcatata atttatataa tatatctgta ataatttctg caatcactat
    14401 tatcataatt atttctccaa tatcaagtat acacttatcg cttccaatta cacatgatca
    14461 tattagagtg tattatcaca ttctctaata ttcaatttat ttttcctaaa atattcgaaa
    14521 taatcgtctt gataacgagt aagtattttt atacgattga taacatagta acaatatttt
    14581 taattatagt aaaaaatcat ctgccttttt catccaccta atcttatttt tcattttatt
    14641 caacatcctt aaataaactt ccgttcct
//
LOCUS       DAZKLM010000065        14625 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3764
            SAMEA115706913-rid22582563.denovo.067, whole genome shotgun
            sequence.
ACCESSION   DAZKLM010000065 DAZKLM010000000
VERSION     DAZKLM010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706913
            Sequence Read Archive: ERR13196647
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14625)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14625)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287823.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287823.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,004
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,849
            CDSs (with protein)               :: 2,849
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 41 of 96
            Pseudo Genes (incomplete)         :: 52 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 15 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14625
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706913-rid22582563.denovo.067"
                     /isolate="VRE3764"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            4..816
                     /locus_tag="ACG842_001921"
     CDS             4..816
                     /locus_tag="ACG842_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HFQ0248453.1"
                     /translation="MLRFFPQKNKYIQEKSEFQFNTDGPIPQHIAIIMDGNGRWAQNR
                     RLPRVAGHKEGMETVKKVTKKASRLGVKVLTLYAFSTENWKRPKDEVSFLMQLPVDFF
                     DTFVPELIKENVKVHVMGYENVLPEHTQDAVRRAIEQTKNNTGMVLNFALNYGSRAEI
                     VTAVQEIAEEVAKGEIHAEEIDDELIAKHLMTGFLPKELQDPELMIRTSGEERISNFL
                     LWQIAYSELYFTKALWPDFDGAHLEEAIASYQNRDRRFGGVKKTEDEKGDQS"
     gene            813..1613
                     /locus_tag="ACG842_001922"
     CDS             813..1613
                     /locus_tag="ACG842_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HFQ0248454.1"
                     /translation="MKQRVITAAVALVLFIPIIWIGGIAVELTAAILAVVGVYELFRM
                     KGLTLLSFEGVLSAVGAVLLVLPKERWFFFLPENVSTFMLFYLIVMILLGTSVISKNT
                     YTIDEAGFPVLVSLYVGIGFQNFVNARTESLAVLIFGLFIVWATDIGAYMVGRRYGQH
                     KLSPDISPNKTIEGALGGIGSAVVVAFLYLLLYPAKELFNHGMFVMLLMTIIFSAVGQ
                     FGDLVESAIKRHYDVKDSGNILPGHGGILDRFDSLLFVFPIMHLFGII"
     gene            1774..3042
                     /gene="rseP"
                     /locus_tag="ACG842_001923"
     CDS             1774..3042
                     /gene="rseP"
                     /locus_tag="ACG842_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HFQ0248455.1"
                     /translation="MKTILTFIIVFGILVIVHEFGHFFFAKRSGILVREFAIGMGPKI
                     YGHQAKDGTTYTLRLLPIGGYVRMAGNGDDETEMAPGMPLSLLLNSDGIVEKINLSKK
                     IQLTNAIPMELSRYDLEDELTITGYVNGDETEVVTYPVDHDATIIENDGTEIRIAPKD
                     VQFQSAKLWQRMLTNFAGPMNNFILAIVLFIILAFMQGGVQVTNTNRVGEIMPNGAAA
                     EAGLKENDEVVSVDGKEIHSWNDLTTVITKNPGKTLDFKIEREGQVQSVDVTPKSVES
                     NGEKVGQLGIKAPMNTGFMDKIIGGTRQAFSGSLEIFKALGSLFTGFSLDKLGGPVMM
                     YQLSSEAANQGVTTVISLMALLSMNLGIVNLLPIPALDGGKLVLNIFEGIRGKPLSQE
                     KEGILTLAGFGFLMLLMVLVTWNDIQRFFF"
     gene            3110..4819
                     /locus_tag="ACG842_001924"
     CDS             3110..4819
                     /locus_tag="ACG842_001924"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HFQ0248456.1"
                     /translation="MRQSKMLIPTLREVPNDAEVLSHQILLRAGYIRQVSAGIYSYLP
                     LANRVLEKLKRIMREEFEKIGAVEMLMPAILPAELWEESGRYETYGPNLYRFKDRNDR
                     KMILGPTHEETFTDLMRNEINSYKKLPLNLYQIQPKYRDEKRPRFGLLRGREFIMKDA
                     YSFHATEESLDETYKDYEKAYTEVFKRCGLEFRSIIGDGGAMGGKDSKEFMAISEIGE
                     DTICYSTESDYAANLEMATSYYVSKKSHETQLELEKVATPNVKSIDEVADFFSVEPQK
                     IIKSVLFIADEQPVLVLVRGDHEVNDVKLKNFLSADFLEEATEEEAQKYLGAEFGSVG
                     PVGVSDDVKVYADRYVQDLANAITGANETGFHYKNVNPERDFTVLSYEDLRFVQEGDP
                     SPDNNGVLAFTRGIEIGHIFKIGTRYSESMGATVLDENGREKFVIMGCYGIGVSRLLS
                     AIVEQHSDEQGINWPKGIAPFDLHIVQMNLKDEYQTSLTNELEEAMTKAGYEVLVDDR
                     NERAGVKFADSDLIGCPIRITVGKKAVDGIVEVKIKKTGEMVEVRKEELANTLPILLN
                     QGE"
     gene            5031..9389
                     /locus_tag="ACG842_001925"
     CDS             5031..9389
                     /locus_tag="ACG842_001925"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HFQ0248457.1"
                     /translation="MSEKRDLFEKLLEQIQLEEAEKNHPLLTAGEMDSVVVHRKSRLW
                     EFTLHFSKILPIMLYRSLTQHLTIAFKDIAQVKLNILADDQTFDEQLLQDYWAQALEN
                     QQCDTPLAQQVLKTQVPVIKDRKVILPIDSTGAISYLKQQYLPLVEELFVSYGFPKFR
                     IEPEVDEQQAERVLKLFEERKQEQAEAFMKQAAESLVVHEQKKKERKEQSPALEGPIH
                     IQLGRNIPADEPTTPMISIVEEERRVTLEGYVFDKEVRELRSKRKILTLKITDYTSSF
                     IVKKFSNGEKDEQVFDAISVGSWLKVRGSIQEDTFVRDLVMNAQDIIEVKHTPRKDYA
                     PEGEKRVELHVHSNMSTMDATNSISDLVAQAGKWGHRAIAITDHGGAQAFPEAHSAGK
                     KAGVKILYGVEANVVDDGVPIAYNDAHEALSEATYVVFDVETTGLSAVYDTIIELAAV
                     KMYKGNVIESFDEFIDPGHPLSRTTVDLTGITDGMVRGSKSEEEVLRMFLEFSKDTIL
                     VAHNAAFDMGFLNTSYARYGIPEAANPVIDTLELARYLYPQFKRFGLGVLSKKFGVSL
                     EQHHRAIYDAEATGHLAWIFVKEAMDNHNMLYHDQLNEHIGEGDSYKRARPFHVTILA
                     KNQAGLKDLFKLISMSNVEYFERVPRIPRSQLKKMRENLLIGSACDKGEIFEAMMQKG
                     VEEARNRAKFYDYIEVMPKAVYAPLIEQELVKNEHDLEEIIQNLVEIGKSLDKIVVAT
                     GNVHYLNEEDAIYRKILINSMGGANPLNRHSLPDVHFRTTDEMLTAFHFLGEETAKEI
                     VVENTNKIADICEEVIPVKDELYTPKIPGSEDEISELSYTKAKQMYGDPLPEIIQKRL
                     KKELNSINGNGFSVIYLIAQKLVHKSNEDGYLVGSRGSVGSSFVATMTGITEVNPLAP
                     HYYCPECQYSEFFEDGTYGSGFDMPEKQCPKCGARLNKDGHDIPFETFLGFHGDKVPD
                     IDLNFSGDYQAEAHNYTKVLFGEDYVYRAGTIGTVADKTAYGYVKGYERDNNLQFRSA
                     EVDRLAKGATGVKRTTGQHPGGIIVIPDYMDVYDFTPIQYPADDQNSEWKTTHFDFHS
                     IHDNVLKLDILGHDDPTVIRMLQDLSGIDPQTIPTDDPEVMRIFAGPEVLGVSQEQIY
                     SKTGTLGIPEFGTRFVRGMLEETHPTTFAELLQISGLSHGTDVWLGNAEELIRRGDAT
                     LAEVIGCRDDIMVYLIHAGLDSGMAFKIMETVRKGQWNKIPDELRETYLSAMKENNVP
                     DWYIDSCSKIKYMFPKAHAAAYVLMALRVAYFKVYFPILYYCAYFSVRADDFDLVSMC
                     KGKDAVKQAMKEITDKGLDASVKEKNQLTVLELANEMLERGFKFGMIDLYKSDAVNFV
                     IEGDTLIAPFRAVPSLGTNVAKQIVEARKDGPFLSKEDLATRGKVSKTLIEYMNDNGV
                     LKDLPDENQLSLFDML"
     gene            9534..10007
                     /gene="rimP"
                     /locus_tag="ACG842_001926"
     CDS             9534..10007
                     /gene="rimP"
                     /locus_tag="ACG842_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HFQ0248458.1"
                     /translation="MSSVVETVTEMVTPILDEQNFELVEVEFVKEGKSWFLRVFIDKE
                     GGIDIEECAFVSEKLSEKLDAADPDPIPQAYFLEVSSPGAERPLKKESDYEKALGDYV
                     HVSLYQPVDGEKQYEGFLQSFDAEQLTLKIRIKTREKELTFDRKNIAKARLAIQF"
     gene            10030..11205
                     /gene="nusA"
                     /locus_tag="ACG842_001927"
     CDS             10030..11205
                     /gene="nusA"
                     /locus_tag="ACG842_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HFQ0248459.1"
                     /translation="MSKEMLNALDALEAEKGISKEIVIDALEAALVSAYKRHYGQAQN
                     VEVEFDQKKGKIHVYAVKEVTEEVMDSQLEVSLKDALLINPAYEIGDTIRFEVTPKDF
                     GRIAAQTAKQVILQRVREAERTIIYNEFSAYEKDIMQGIVERQDKRYIYVNLGKIEAV
                     LSKQDQMPNEFYQPHDRIKVYVSRVENTSKGPQVFVSRSHPDLLRRLFEQEVPEVYDG
                     IVEIVSIAREAGDRSKVAVRSNDPNIDAVGTCVGPKGQRVQAIVNELKGENMDIVEWN
                     EDPAVFIANALNPAQVMDVIFDETNPKACTVVVPDYQLSLAIGKRGQNARLAAKLTNH
                     KIDIKSESDMTEFYENQEQQKETEELHDEAIVQSDLTDDEYETIAFNNETVEEKPEA"
     gene            11227..11520
                     /locus_tag="ACG842_001928"
     CDS             11227..11520
                     /locus_tag="ACG842_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HFQ0248460.1"
                     /translation="MKQRKIPLRKSVVSGEMKPKKEMVRITRSKEGEVSIDPTGKMPG
                     RGAYVSLEPEEVQQAWDKKILDRVLETTLTDEFYQELLDYVTHQKARKELFGK"
     gene            11517..11828
                     /locus_tag="ACG842_001929"
     CDS             11517..11828
                     /locus_tag="ACG842_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ-related RNA-binding protein"
                     /protein_id="HFQ0248461.1"
                     /translation="MNTVNRQKAMNLIGLAMRAGKLITGEELTIAEIRKQKAKLVFVA
                     TDASENTRKKIKDKSSYYEVPCFELFSEAEITQMIGKPRKVFGIMDAGFAKKTKELIE
                     G"
     gene            11841..14147
                     /gene="infB"
                     /locus_tag="ACG842_001930"
     CDS             11841..14147
                     /gene="infB"
                     /locus_tag="ACG842_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HFQ0248462.1"
                     /translation="MGNKRIYELAKELKKSSKDVVEKAQQLGIDVKNHMGAITSNDEK
                     KLQQAFKNPSTKQQAAQQASRKPANQQPNDQRKKETTNNQQKNKNNRSYQDRGQGSGQ
                     VNQGKKPTTNQNNTGNTSSQNRQGTSQGGNQNRTNNNNKPNNNNRGKFNNNNRNRYNK
                     KGKKGKQQQSTKPAVPPRKFRELPDVLEYTEGMNVADIAKKIHREPAEIIKKLFMLGV
                     MVNQNQALDKDTIELLATDYGMEPQEKVQVDIADIDKFFEAEEVDPDKLVSRPPVVTI
                     MGHVDHGKTTLLDTLRHSRVTSGEAGGITQHIGAYQIDIDGKPITFLDTPGHAAFTSM
                     RARGASITDITILVVAADDGVMPQTVEAINHAKAAGVPIIVAVNKIDKPGANPQHVMQ
                     ELSEYELIPEAWGGETIFVEISAKFGQNIEELLEMILLVAEVEDLKADPTQRAIGTVI
                     EARLDKGKGPVSTLLVQEGTLHVGDPIVVGNTYGRVRVMTNDLGRRDKEAGPATPVEI
                     TGLNDVPQAGDRFVVFEDEKTARAAGEERAKRALLENRAATSRVTLDNLFESLKEGEL
                     KEVNVIIKADVQGSAEALAASLKKIDVEGVRVKIVHSAVGAINESDVTLAAASNAIII
                     GFNVRPTPQAKVQADTEEVDIRLHRIIYKAIEEIETAMKGMLDPEFEEKITGQMIVRE
                     TFKVSKVGTIAGAYVTEGYIRRDSGVRVIRDGIVIYEGQLASLKRFKDDVKEVKMGYE
                     CGAMIEKFNDIKVDDVIEGFIMEEVKND"
     gene            14174..14521
                     /gene="rbfA"
                     /locus_tag="ACG842_001931"
     CDS             14174..14521
                     /gene="rbfA"
                     /locus_tag="ACG842_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HFQ0248463.1"
                     /translation="MANYRDRRVGQEILKEVNDILRKKVRDPRVENVTITDVKVTGDL
                     QQATIYYSILSDLASDKKTAQDGLNKASGLIRRELGHNLSIYKTPELFFELDESVVYG
                     NHIDELLRDLNKD"
BASE COUNT         4983 a         2598 c         3255 g         3789 t
ORIGIN      
        1 agaatgttac gtttttttcc gcaaaaaaac aaatatattc aagaaaaaag cgaatttcaa
       61 tttaataccg atggaccaat tcctcagcac attgccatca tcatggacgg caatggtcgt
      121 tgggcgcaaa accgtcgttt gccacgagtt gctggccata aagaaggcat ggaaacagta
      181 aagaaagtca caaaaaaagc atcaaggctt ggagtgaaag tgttgacgct ttatgccttt
      241 tcgactgaaa actggaagcg accaaaagat gaagtaagtt ttctcatgca actacctgtc
      301 gacttctttg atacgttcgt accagaattg attaaagaaa atgtcaaagt tcatgtcatg
      361 ggttatgaaa acgtattgcc agaacatacg caagatgctg taagacgtgc aattgagcaa
      421 acaaaaaaca atactggaat ggtattgaat tttgctttga attatggaag tagagcagag
      481 atcgtgactg ctgtccaaga aattgccgaa gaagtagcaa aaggagaaat acacgcagaa
      541 gaaatcgatg atgaattgat tgctaagcat ttaatgacag gtttcttgcc taaagagtta
      601 caagatcctg agctgatgat ccgtacaagt ggagaagagc gaattagtaa ttttctgtta
      661 tggcagatcg catatagtga gctgtatttt acaaaagcgc tttggcctga ttttgatggt
      721 gcgcatcttg aagaagcaat cgcctcttat caaaatcgag accgtcgatt cggcggtgta
      781 aaaaaaacag aagatgagaa aggggatcag tcatgaaaca acgtgtgatc acagcagctg
      841 tggcgctagt cttgtttatt ccgattatat ggatcggagg catagcggtt gagcttacag
      901 cagcaatctt agcggtagta ggagtttacg agctgttccg gatgaaagga ttgacactct
      961 taagttttga aggagtttta tccgccgttg gtgcagtgtt acttgttttg ccaaaagaaa
     1021 gatggttttt ctttcttcca gagaatgtaa gtacatttat gttattttat cttatagtta
     1081 tgattctttt aggaacatca gttatttcta aaaatacgta tacgattgat gaagcaggtt
     1141 ttcctgtgct tgtcagcttg tatgtgggaa taggtttcca gaattttgtc aatgcacgaa
     1201 cagaaagctt ggctgttttg atatttggtc tatttattgt ttgggcaact gatatcggag
     1261 catatatggt tggtcgacgt tatggtcagc ataagttaag tcctgatatt tcgcccaaca
     1321 aaacgattga aggtgcgctc ggtggtatcg gtagtgctgt tgtagtcgca tttctttatt
     1381 tattgctata cccagcaaaa gaactattca atcatggtat gtttgtcatg cttctgatga
     1441 cgattatttt ttcagcagtc ggacaatttg gtgatttggt cgaatctgca atcaaacgtc
     1501 attatgatgt gaaagactcg ggaaatatct taccaggcca tggtggaatc ttagaccgtt
     1561 tcgacagttt gctgtttgta tttcctatca tgcatttatt tggcattatt tagtaagtat
     1621 acctgcgttt ctttgtgaat ttcataaaag acgcaggttt ttatttttca ttatattgat
     1681 actattggtg tctttggtat actatggtaa aattatagtg ttttatcgtt gttttataaa
     1741 aaacacaaaa aacaagtaaa ggagaaaaaa gctatgaaaa cgattctgac atttatcatc
     1801 gtttttggta tattagtgat tgttcatgag tttggtcatt tcttctttgc gaaacgatca
     1861 ggaatcctcg tccgcgaatt tgctatcggt atgggtccga aaatttatgg gcatcaagca
     1921 aaagacggga caacatatac gcttcgccta ttacctattg gcggttatgt tcgaatggca
     1981 ggcaatgggg atgatgaaac agaaatggca ccaggaatgc ctctttctct tttactaaat
     2041 tctgacggca tcgtagagaa gataaattta agtaagaaaa tccaattgac aaatgctatc
     2101 ccgatggaat tgagccgtta tgatttagaa gatgagctga ctatcactgg ttatgtgaat
     2161 ggagacgaaa cagaagtagt aacctaccct gttgatcacg atgcgacgat tattgaaaac
     2221 gacggaacag aaatcagaat tgcgccaaaa gacgtacagt tccaatcggc taaattgtgg
     2281 cagcgtatgc tgaccaactt tgctggaccc atgaataact ttattttggc aatcgtgtta
     2341 ttcatcattc tggcatttat gcaaggtggt gttcaagtga cgaacactaa tcgcgtagga
     2401 gagatcatgc ctaatggagc tgcggcagaa gcaggcttaa aagaaaatga tgaggtagtc
     2461 agtgtggacg gaaaagaaat ccattcatgg aatgatctga caacagtcat tacgaaaaat
     2521 ccaggtaaaa cattagactt taagatcgaa cgagaaggtc aggtgcaatc tgtcgacgtg
     2581 acaccgaaat ctgtagagtc aaatggcgaa aaagttggcc aacttggtat aaaagcccca
     2641 atgaatacag gttttatgga taagatcatt ggagggacgc gtcaagcttt cagcggttca
     2701 ttggaaattt tcaaagcctt aggatcacta tttacaggtt tcagtttaga taaattaggc
     2761 ggacctgtca tgatgtacca attgtcttca gaagcagcaa atcaaggtgt aacaactgtt
     2821 attagtttga tggcgcttct ttcaatgaat ctcggaatcg tcaatctgct tccgattcct
     2881 gccttagatg gcgggaaatt agtcttgaat atttttgaag ggattcgcgg gaaaccatta
     2941 agtcaagaaa aagaaggaat attgacatta gctggctttg gatttttaat gttgttgatg
     3001 gtgctagtta catggaacga tattcaacga ttctttttct agaaaaagtt gacaaatagg
     3061 gtataataac aaacgataac tattttgagg aaataaagga gtatatagaa tgagacaatc
     3121 aaaaatgttg attccaacat tacgcgaagt tccaaatgat gcagaagtat taagccatca
     3181 aatcttgcta agagctggat atatccgcca agtatcagcg ggtatttatt catatctgcc
     3241 attagcaaat cgtgtattgg aaaaactgaa acgcattatg cgggaagaat ttgaaaaaat
     3301 cggagcggta gaaatgctga tgcctgccat cttgccagca gaactttggg aagaatccgg
     3361 gcgttacgaa acatatggac caaacttgta tcgtttcaaa gaccgcaatg atcgaaaaat
     3421 gatccttgga ccaactcacg aagaaacttt tactgatttg atgcgtaatg aaatcaattc
     3481 atataagaaa ttacctttaa atctttatca aatccaacct aaatatcgtg acgaaaaacg
     3541 acctcgcttc ggtttgcttc gcggaagaga gtttatcatg aaagatgcat attctttcca
     3601 tgcaacggaa gaaagtttgg acgagacata taaagattac gaaaaagcct ataccgaagt
     3661 attcaagcgt tgtggcttag aatttcgctc gatcatcgga gacggcggcg caatgggcgg
     3721 gaaagactca aaggaattca tggcgatctc agaaatcggg gaggatacga tctgttattc
     3781 gactgaaagt gattatgctg caaacttgga gatggctaca agctactatg tatcaaaaaa
     3841 atcgcatgaa acacagcttg aattggagaa agtagctaca cctaacgtaa aatcaattga
     3901 cgaagtagca gatttctttt ctgttgaacc tcaaaaaatc atcaagtctg tattgttcat
     3961 tgcggatgaa caacctgtcc ttgttttggt acgtggagac catgaagtca atgacgtaaa
     4021 actgaaaaac tttttatctg ctgacttttt agaagaagca acagaagaag aagcacaaaa
     4081 atatctaggt gctgagtttg gttctgtcgg accagtcggt gtttcagatg acgtaaaagt
     4141 atatgcagac cgttatgtcc aagatttagc gaatgccatt actggagcga acgaaacagg
     4201 attccactat aaaaatgtga atccagaacg cgatttcact gtgctttctt atgaagatct
     4261 tcgttttgta caagaaggag atccttcacc agacaacaac ggtgttcttg cctttactcg
     4321 aggaatcgaa atcggacata tattcaaaat cggcactcgt tacagtgaat ctatgggagc
     4381 aactgtacta gacgaaaatg gacgagaaaa atttgtgatt atgggctgtt atggtatcgg
     4441 tgtaagtcgt ttattatcag caatcgtaga acaacattca gatgaacaag gaatcaattg
     4501 gccaaaagga attgcgccat ttgatctgca tatcgtccaa atgaatctaa aagacgaata
     4561 ccaaacaagc ctgacgaatg aactagaaga agcgatgaca aaagctggct atgaagtact
     4621 ggttgatgac agaaatgaac gcgcaggagt gaaattcgcg gattctgatt tgattggctg
     4681 tccaatcaga atcactgtcg gtaaaaaagc agtggatgga atcgtagaag tgaaaatcaa
     4741 gaaaactggc gaaatggttg aagtacgtaa agaagaattg gcgaatactt taccaatcct
     4801 cttgaaccaa ggcgaataat aagcgttaaa acacagaaaa taaaaatagc tgaatcagct
     4861 gtttttgaca atctgatgat tcgcttttaa aattggaact gcgatttgaa tcgcagttcc
     4921 aattttttaa tgtaaaactc agaaaaaacg agtatactaa aaggtactga aaaagaagag
     4981 gataagatgc gatcaattgc attgtttctc atcagaaagg gcgtatatct ttgtcagaaa
     5041 aacgagattt atttgaaaaa ctgttggaac aaatccaact agaggaagca gaaaaaaatc
     5101 atccactgct tactgctggt gaaatggata gtgtagtggt ccatcgtaaa tcgcgtttat
     5161 gggaatttac gctacatttt tccaaaattt tgcctattat gctatatcgg tcattgactc
     5221 agcatctgac tatcgctttt aaagatattg cacaagtcaa actgaatatc ttagcagatg
     5281 accagacatt cgatgaacag ctcttgcaag attactgggc ccaagctttg gaaaatcagc
     5341 aatgcgatac accactagca cagcaagtat tgaaaacgca ggtacctgtg ataaaagatc
     5401 gtaaagtcat cttgccaatc gacagtacag gagctatttc ttatttgaag caacaatact
     5461 tgccattagt agaagagttg ttcgtcagtt acggattccc aaaatttcgt atcgaaccag
     5521 aagtggatga gcagcaagcg gaacgagtat tgaaactttt tgaagaacgc aaacaagaac
     5581 aggcagaagc ctttatgaag caagcagctg aaagtctagt tgtccacgaa caaaagaaaa
     5641 aggaaagaaa agagcaatcg ccagctcttg aaggacccat ccacatccag ctaggacgaa
     5701 atattccagc cgatgaacca acgacaccaa tgatcagtat cgttgaagaa gaacgaagag
     5761 tcacacttga ggggtacgta ttcgacaaag aagtcagaga actacgttca aaacgcaaga
     5821 tcttgacttt gaaaatcaca gattatactt catcatttat cgtcaaaaaa ttctcgaacg
     5881 gcgaaaaaga tgaacaagta tttgatgcga tcagtgtcgg tagctggtta aaggtacgag
     5941 gaagtattca agaagatacg tttgtccgtg atttggtcat gaatgcgcaa gatatcatag
     6001 aagtcaaaca tacacctcgc aaagattatg ctcctgaagg ggaaaaacga gtagagcttc
     6061 atgttcatag caatatgagt acgatggatg caacaaacag catctctgat cttgtggcac
     6121 aagcagggaa atggggacac cgagctatcg ccattacaga ccacggtggt gcgcaagcct
     6181 ttccggaagc acatagtgct ggaaaaaaag caggtgtgaa aatcttatac ggcgtggaag
     6241 cgaatgtcgt agatgatggt gtcccaattg cttataatga tgcacatgaa gcattgagtg
     6301 aagccacata tgtcgtattt gacgtggaaa cgacaggatt gtctgcagta tatgacacga
     6361 tcatcgagct agccgcagtc aaaatgtata aaggaaatgt gatcgaaagc tttgatgaat
     6421 ttatcgatcc aggacatcct ttgtcacgaa caacggtaga tctgactggt atcacggatg
     6481 gaatggttcg aggatcaaag tcggaagaag aagtattgcg gatgttcctt gaattttcaa
     6541 aagatacaat tttggtagcc cacaacgcgg cttttgatat ggggttcttg aatactagct
     6601 acgcaaggta tggtattcct gaagcggcca atccagtcat tgatacgttg gaattagcta
     6661 gatatctgta tcctcaattc aaacgtttcg gattaggtgt gttatctaaa aaatttggtg
     6721 tcagcttgga acagcatcac cgagcaatct atgatgctga agcaactggt cacttagcat
     6781 ggatctttgt gaaagaggca atggacaatc ataacatgct ttatcatgat cagctaaatg
     6841 agcatatcgg agaaggagat tcttacaaaa gggctcgacc tttccatgta acgattttag
     6901 caaaaaatca ggcaggactg aaagatttgt tcaaactgat ttcaatgtcg aatgtcgaat
     6961 acttcgaacg cgtcccacga ataccacgct cccaattgaa aaagatgcgt gaaaatctat
     7021 tgatcggctc agcttgtgat aagggagaaa tattcgaagc catgatgcaa aaaggggtag
     7081 aagaggcaag aaaccgagca aaattctacg attatattga agttatgcca aaagcggtct
     7141 atgcgccttt gatcgagcaa gaattggtaa aaaatgaaca tgatttggaa gaaattatcc
     7201 aaaatttggt agaaatcgga aaaagtttgg ataaaatcgt tgttgcaaca ggtaatgtac
     7261 attatctgaa cgaagaagat gccatttacc gaaaaatttt gatcaattct atgggcggag
     7321 cgaatccgtt gaatcgtcat agtttgccgg atgtccattt ccgaacaacc gatgagatgc
     7381 taactgcatt tcacttttta ggggaagaaa cagcgaaaga gatcgtagta gaaaatacga
     7441 ataaaatcgc agatatctgc gaagaagtga ttccagtcaa agatgaactt tatacaccga
     7501 agattccagg ttcagaagat gaaatatcag aattgagtta cacaaaagca aaacaaatgt
     7561 acggagatcc gttacctgag atcattcaaa aacgattaaa aaaagagttg aattctatta
     7621 atggtaatgg cttctcggtt atttatctga tcgcccaaaa actagtgcat aaaagtaatg
     7681 aagatggcta tctagtaggt tcacgtggct ctgttggctc tagctttgtt gcgacgatga
     7741 caggaatcac tgaagttaat ccgttagccc ctcattatta ttgtccagag tgccaatatt
     7801 ccgagttttt tgaagatggt acgtatggtt ctggatttga catgccagaa aaacaatgtc
     7861 caaaatgtgg tgcacgctta aacaaagacg gtcatgatat tccatttgag acatttttag
     7921 gattccatgg agacaaagtg cccgatattg atttgaactt ctcaggtgat tatcaagcag
     7981 aagcccataa ctatacgaaa gtattgtttg gcgaagacta tgtttaccgt gccggaacga
     8041 ttggtacagt cgccgacaaa acagcatatg gctatgtaaa aggatacgaa agggacaata
     8101 atctgcagtt tcgaagtgca gaagtcgaca gattagcaaa aggcgctact ggagtcaaac
     8161 ggacaaccgg ccagcatcca ggggggatca tcgttattcc cgattatatg gatgtctatg
     8221 attttacgcc gatccaatat ccagcagacg atcaaaattc ggaatggaaa acgactcatt
     8281 ttgattttca ctcgattcac gataatgtat tgaaattaga tattctaggg catgatgacc
     8341 caactgttat tcggatgctt caagatttat cagggatcga tccacaaacg atccctacgg
     8401 atgacccgga agtgatgcgg atatttgccg gtcctgaagt acttggagta agtcaggaac
     8461 aaatttattc caaaacaggg acattaggta ttccagaatt cggcactcgt tttgttcgag
     8521 ggatgctaga agaaacgcat cctacaactt ttgcagaact actgcaaatt tccggactat
     8581 ctcatggtac agacgtatgg ttaggaaatg cggaagaatt gattcgacga ggagatgcga
     8641 cactggcgga agtaatcggc tgtcgtgatg atattatggt ttatttgatc catgctggat
     8701 tagatagcgg gatggcattt aaaatcatgg aaactgttcg taaaggacaa tggaacaaga
     8761 tccctgatga attgcgagaa acctatctat cggcgatgaa agaaaataat gtgcccgact
     8821 ggtatatcga ttcttgttca aaaatcaaat atatgtttcc gaaagcccat gctgcagctt
     8881 acgtattgat ggctcttcgt gtcgcttatt tcaaggttta tttccctatt ttgtattact
     8941 gtgcatactt ttctgtacgt gcagacgact ttgatctagt ctccatgtgc aaaggaaaag
     9001 atgctgtgaa acaagcaatg aaagaaatca cggacaaagg attagatgct tccgtaaaag
     9061 aaaagaatca actgacagtg ctggagttag ccaatgaaat gctggaacga ggattcaaat
     9121 ttggaatgat tgatttgtat aaatcggatg ccgttaactt tgttattgaa ggtgatacgt
     9181 tgatcgcacc atttagagct gtcccaagtt tgggaaccaa cgtggcaaaa caaatcgttg
     9241 aagcaagaaa agacggtcca tttttatcca aagaagatct tgccacccgc ggtaaagttt
     9301 caaaaacatt gatcgaatat atgaacgaca atggcgtatt gaaagatcta ccagatgaaa
     9361 atcaattatc gttattcgat atgctataag catctgtttt cctgtctgct tgtaatttta
     9421 agcagagtat ggtatagttc ttataggtaa tgatactaac tgtgagtgag cggatttttc
     9481 gctcactctt tttaatggaa ttacgcactt aattttttaa ggaggcgaaa cttttgagta
     9541 gcgtcgttga aacagtcaca gaaatggtga caccgatttt agatgaacaa aattttgaac
     9601 ttgtcgaagt cgaatttgta aaagaaggaa aaagctggtt cttacgagta tttattgaca
     9661 aagaaggcgg tatcgatatc gaagaatgcg cctttgtcag tgagaaactg agtgaaaaac
     9721 ttgatgcagc agatccagat ccgattcctc aagcttattt cctggaggta tcctccccgg
     9781 gagcagagcg gccattgaaa aaagaaagcg actatgaaaa ggcacttggt gattatgtcc
     9841 atgtctcctt ataccaacca gtagatggcg aaaaacaata tgaaggattt cttcaatcat
     9901 ttgatgctga acagcttaca ttgaaaatac gcataaaaac acgagaaaaa gaattgactt
     9961 ttgaccgaaa aaatattgca aaagctcgct tagctatcca attttaatca cggagaggaa
    10021 tcagaaaaaa tgagtaaaga aatgttaaat gcgttagacg ctttagaagc tgaaaaaggg
    10081 atctcaaaag aaatcgtgat cgatgcttta gaagcagcat tggtttctgc atacaaacgt
    10141 cactatggcc aagcacaaaa cgtcgaagtg gaatttgatc agaaaaaagg aaagatccat
    10201 gtctatgcgg taaaagaagt aacggaagaa gtgatggatt cacagttgga agtctcattg
    10261 aaagatgcac ttttaatcaa tccagcatat gagatcggtg atacgatccg ttttgaagta
    10321 acaccaaaag attttgggcg gatcgctgcg caaacggcta aacaagttat tttacagcgt
    10381 gtacgtgaag cagaaagaac gattatttat aacgaattca gtgcttatga aaaagacatc
    10441 atgcaaggaa tcgtcgaacg ccaggataaa cgttatattt atgtcaatct tggtaaaatt
    10501 gaggcagttc tttctaaaca agatcaaatg ccaaatgaat tttatcaacc acatgatcga
    10561 atcaaagttt atgtttcccg agtagaaaat acgtctaaag gtccgcaagt atttgttagc
    10621 cgcagccatc cagacctatt gcgccgctta tttgagcaag aagtaccaga agtctatgat
    10681 ggaatcgttg aaattgtcag catcgcccgt gaagctggcg atcgttcaaa agtggcagtt
    10741 cgctcaaatg atccaaatat cgatgcagtc ggtacatgtg tcgggccaaa gggacaacga
    10801 gttcaagcaa tcgtcaatga attaaaaggc gaaaacatgg atatcgtcga atggaatgaa
    10861 gatccagctg tattcattgc aaatgcattg aatcctgcac aagtcatgga tgtgatcttc
    10921 gatgagacaa atccaaaggc ctgcacagtt gtcgttccag actaccaatt atcgctagca
    10981 atcggtaaac gcggacaaaa cgcacgttta gctgctaaat tgactaacca taaaatcgat
    11041 atcaaatcag aatctgacat gacggaattt tatgaaaatc aagaacaaca aaaagaaaca
    11101 gaagaactgc atgatgaagc aatcgttcag tcagatttaa cagacgatga atatgaaaca
    11161 attgcattca acaatgaaac ggtcgaagaa aaaccagaag cttaacttta gtaaggaggc
    11221 aaagagatga aacaaaggaa aattccttta cgcaaatccg tggtttcagg tgaaatgaaa
    11281 cctaaaaaag agatggtccg aatcacccgt tcaaaagaag gggaagtatc gatcgatcca
    11341 actggaaaaa tgcctggacg aggagcgtat gtctcacttg agccggaaga agtacaacaa
    11401 gcttgggata aaaaaattct cgatcgagta ctcgaaacaa cattaacaga tgagttttat
    11461 caagaacttc ttgactatgt cactcaccaa aaagcacgta aagagttatt tggaaaatga
    11521 atacagttaa tcggcaaaaa gccatgaatc tcatcggtct agcgatgcgc gcgggaaaac
    11581 taatcacggg tgaagaactg accattgctg aaatccgcaa acaaaaagca aaattggtgt
    11641 ttgtggcaac cgatgcaagc gagaatacaa gaaaaaagat aaaagataag agttcatatt
    11701 atgaagttcc ttgctttgag ctattttctg aagcagaaat cacgcagatg atcggaaaac
    11761 ctcgcaaggt attcggaata atggatgccg gatttgcgaa gaagaccaag gagctaattg
    11821 aaggttagga aggtgattgc atgggcaaca aaagaatcta tgaattagca aaagaactca
    11881 aaaaatctag taaagacgtc gtggaaaaag cacaacaact tggcatcgat gtgaaaaacc
    11941 atatgggtgc gattactagc aatgatgaga agaaattgca acaagcgttc aaaaacccaa
    12001 gcacgaaaca acaagctgca caacaagcaa gcagaaaacc agccaatcaa cagccgaacg
    12061 atcaacgtaa aaaagaaaca acgaacaatc aacaaaaaaa taaaaataac cgcagttatc
    12121 aagatcgcgg tcaagggagc ggtcaagtga atcaaggaaa aaaaccaaca acaaaccaaa
    12181 ataatacggg aaataccagc agccaaaatc gtcaaggaac gagccaaggc ggcaatcaaa
    12241 accgtactaa caataacaac aaaccaaaca ataacaatcg cggcaaattt aataataata
    12301 accgtaatcg ttataataaa aaaggtaaaa aaggcaaaca gcaacaatca actaaaccag
    12361 ctgtaccacc acgcaaattc cgtgaattgc cagatgtatt agagtatact gaaggtatga
    12421 acgtagcaga tattgcgaaa aaaattcatc gtgagccagc agaaatcatt aaaaaattat
    12481 ttatgcttgg tgtcatggtc aatcaaaacc aagcactgga taaagatacg attgaactgt
    12541 tagcgacgga ctatggcatg gaaccacaag aaaaagtcca agttgacatt gctgatatcg
    12601 ataaattctt cgaggcagaa gaagtcgatc cagataaatt agtctcacgt ccgccagtcg
    12661 taacgattat gggacacgta gaccatggga aaacaacttt attggataca ttacgccatt
    12721 cacgtgtaac aagcggcgaa gccggcggaa tcacacagca tatcggtgcc taccagatcg
    12781 atattgatgg taaaccaatc actttcttgg atacaccagg acatgcggcc tttacaagta
    12841 tgcgtgcacg tggagcaagt atcacagata tcacgatctt agttgttgct gcagatgatg
    12901 gcgtcatgcc acaaacagtt gaagcaatca accatgccaa agctgcaggc gttccaatca
    12961 tcgtcgctgt aaataaaatt gataaaccag gtgcaaaccc tcaacatgtt atgcaagaat
    13021 taagtgagta tgagttaatt cctgaagcat ggggtggaga aacgatcttt gttgagatct
    13081 cagcgaaatt cggccaaaac atcgaagaat tgctggaaat gatcctgtta gtcgcagaag
    13141 tagaagattt aaaagcagat ccaacacaac gcgcaatcgg aacagtgatc gaagcacgtc
    13201 ttgataaagg taaaggtcct gtgtcaacct tacttgtcca agaaggaaca cttcatgttg
    13261 gtgacccaat cgttgtcggt aatacttacg gacgtgtccg tgtaatgaca aacgatcttg
    13321 gccgtcgcga taaagaagca ggacctgcaa cgccagtaga aatcactggc ttgaatgatg
    13381 tacctcaagc aggtgaccgt ttcgtagtct tcgaagacga aaagacagca cgtgcagcgg
    13441 gtgaagaacg cgcgaaacgt gcattattag aaaaccgtgc tgccacaagc cgtgtgactt
    13501 tggataactt atttgaaagc ctgaaagaag gcgaattgaa agaagttaac gtaatcatca
    13561 aagcagatgt acaaggttca gcagaagcac tggctgcatc attgaagaag attgatgtgg
    13621 aaggtgtccg tgtaaaaatc gtccattcag ctgtcggagc aatcaatgaa agtgatgtaa
    13681 ccttagcagc agccagcaac gctattatca tcggtttcaa cgttcgtcct acaccgcagg
    13741 caaaagtgca agctgatact gaagaagtcg atattcgtct tcaccgtatc atctataaag
    13801 ccatcgaaga aatcgaaact gcgatgaaag ggatgctaga ccctgaattt gaagaaaaaa
    13861 tcaccggtca aatgatcgtt cgtgaaacct tcaaagtttc taaagttggt acgattgcag
    13921 gtgcctatgt aacagaagga tatattcgta gagatagcgg tgtccgtgta atccgtgacg
    13981 gaatcgtaat ctatgaaggc caacttgcaa gtctgaaacg attcaaagat gacgtgaaag
    14041 aagtcaaaat gggttatgaa tgtggtgcaa tgattgaaaa attcaatgac atcaaagtag
    14101 atgacgtaat tgaaggattt atcatggaag aagtcaaaaa tgactaaaaa ctgataaaag
    14161 gaggccaatc attatggcta actaccgtga ccgccgagtc ggtcaagaaa tcttaaaaga
    14221 agtcaatgat attttgcgca aaaaggtccg tgaccctcgt gtggagaacg taacgatcac
    14281 agatgtgaaa gttacggggg acttacagca agcaacgatc tattacagca ttttatctga
    14341 cttagcatca gataagaaaa cagcacaaga tggattgaat aaagcatcag gattgattcg
    14401 tcgagagtta gggcacaatc taagtattta taagacacct gaattgttct ttgaacttga
    14461 tgaatcagtt gtctatggca accatatcga tgaattgctt cgcgacttga acaaagacta
    14521 atcaaaaact cccgcaaatc ctgtgaaatc aggctctttg tcaataagga ctgatgagtt
    14581 gtgcaaaatc aaatctgagt cagaatgaac cacattctgg ctcag
//
LOCUS       DAZKLM010000066        14620 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3764
            SAMEA115706913-rid22582563.denovo.068, whole genome shotgun
            sequence.
ACCESSION   DAZKLM010000066 DAZKLM010000000
VERSION     DAZKLM010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706913
            Sequence Read Archive: ERR13196647
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14620)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14620)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287823.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287823.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,004
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,849
            CDSs (with protein)               :: 2,849
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 41 of 96
            Pseudo Genes (incomplete)         :: 52 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 15 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14620
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706913-rid22582563.denovo.068"
                     /isolate="VRE3764"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(189..755)
                     /locus_tag="ACG842_001932"
     CDS             complement(189..755)
                     /locus_tag="ACG842_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFQ0248464.1"
                     /translation="MLQTALHFSHTLLQEILQPGDHVVDATMGNGHDTVFLAEHIGKT
                     GHVYSFDIQQQAIDATRERLEQRQLEERVSLFLQGHETLGEVIAEQQNLKAGIFNLGY
                     LPKSDKAIITMPETTRTAMEEILKRLVPRGRLILVVYYGHEGGEKELDMVDSFCSKLP
                     QETYNVLNYRFINQKNQPPILYCIEKKR"
     gene            complement(755..1711)
                     /locus_tag="ACG842_001933"
     CDS             complement(755..1711)
                     /locus_tag="ACG842_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390904.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HFQ0248465.1"
                     /translation="MTFPYSEGNKRYHSWNYALRNEFGGKIFKVPIDGGFDCPNRDGT
                     VAHGGCTFCSVSGSGDMIVAPEDPLPIQFQKEIDMMHQKWPHVQQYIVYFQNFTNTHA
                     PVEIIKERFEQVVNLPGVVGLSVGTRPDCLPDEVVDYLAELNERMYLWVELGLQTTFE
                     ETSKLINRAHDYQTYLDGVAKLRKHNIRVCTHLINGLPGESLEMMKENVRRTILDSDI
                     QGIKLHLMHLMRKTRMLRDYHEGRLQLMSRPDYVNVICDQLEMIPKEIIIHRLTGDAP
                     WDSLIGPMWSLKKWEVLNAIDEELLRRDSFQGKYDVRKKVSV"
     gene            complement(1711..2367)
                     /locus_tag="ACG842_001934"
     CDS             complement(1711..2367)
                     /locus_tag="ACG842_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HFQ0248466.1"
                     /translation="MKYKTSFQVAGSFFLVLFFILGWIVKFHLSWLSGFDQWFTGIVR
                     ASYPDLNSYQLFMTKFGNPLTVIILFACAALWMWYKKKRAETFWFVSGFIVIAGIINP
                     VIKLFFMRERPSLAHLVVETSYSFPSGHAAGSMILYGSLLFLLPAFVQKRTLRTLLQL
                     LMMFIIVSVGISRIYLGVHFPTDIIGGYLLSLSWLCLTYPVYREKRKQIDNSYRKKDF
                     "
     gene            2737..4755
                     /locus_tag="ACG842_001935"
     CDS             2737..4755
                     /locus_tag="ACG842_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFQ0248467.1"
                     /translation="MENIARKERRRLEQTKRFRKAKRGAAIVGTAMVGCSVAAPLVQP
                     VPVQADETPVQFGARINTAAFITEIATYAQPIAQANDLYASVMIAQAVVESGWGSSAL
                     SQAPYYNLFGIKGSYQGQTVYMDTLEFLNNQWVNKKEPFRQYPSFAESFNDNAYVLRN
                     TSFGNGYYYAGTWKSNTKSYTDATACLTGRYATDPGYAGKLNNIIATYGLTQYDTPAS
                     GNAGGGATTGGNNTGSAGSTGGSSNSGNTNSGSATSTTYTVQSGDSVWGISNKFGITM
                     DQLIQWNNIQNNFIYPGQKLTIKGSQENGSSTNNSGNNTNSSGNAGTSNGGQTTGAKY
                     TVQSGDSVWKISNDHGITMAQLIEWNNIKNNFVYPGQQLIVSNGGSSNAGANTGSNTG
                     NTSGSSSSSSSSSNTASGTKYTVKAGDSVWSVSNKYGITMAQLIQWNNIQNNFIYPGQ
                     QLTVSNSGSTNSTSGNTGSSTNTNNSSSVNTGSSSSQGTYTVKAGESVWSVANKNGIT
                     MDQLIEWNNIKNNFIYPGQQLIVKGGTSANTNTGSTTSAAKPNTPNTSATTSTSSTGN
                     TMYTVKAGESVWSVANKHHITMDQLIEWNNIKNNFIYPGQQVIVKKGSSQSTNQQSVT
                     GSKTYTVKSGESVWSVADSHGITMAQLIEWNNIKNNFIYPGQTLIVAK"
     misc_binding    4825..5031
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            5182..7596
                     /gene="leuS"
                     /locus_tag="ACG842_001936"
     CDS             5182..7596
                     /gene="leuS"
                     /locus_tag="ACG842_001936"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HFQ0248468.1"
                     /translation="MSYNHKEIEKKWQKYWAKTNTFNTHDDPEKPKFYALDMFPYPSG
                     QGLHVGHPEGYTATDILSRVKRSQGYNVLHPMGWDAFGLPAEQYALDTGNDPAEFTQK
                     NIETFRRQINSLGFSYDWNREVNTTDPEYYKWTQWIFTKLYEKGLAYEAEVAVNWVPE
                     LGTVISNEEVIDGKSERGGYDVVRKPMRQWMLKITAYADRLLDDLELVDWPESIKEMQ
                     RNWIGRSVGANIEFKVAGTDKSYTVFTTRPDTLFGATYSVLAPELDLVREITTPEQKA
                     AVEAYIEETAKKSDLKRTDLAKEKTGVFTGAYAINPVNGKEIPIWIADYVLSSYGTGA
                     IMAVPAHDERDFEFAQTFGLEILPVIEGGDVEKAAYTEDGTHINSEFLDGMNKQEAID
                     KMNEWLEENGVGKKEVSYRLRDWLFSRQRYWGEPIPIIHWEDGTVTAVPEEELPLRLP
                     KTKNIKPSGTGESPLANIEEWVNVVDPVTGKKGRRETNTMPQWAGSSWYYLRYIDPHN
                     KKELADYEKLKRWLPVDIYIGGAEHAVLHLLYARFWHKFLYDIGVVPTKEPFQKLYNQ
                     GMILGENNEKMSKSRGNVVNPDDVVETYGADTLRMYEMFMGPLDASIAWSENGLEGSR
                     KFLDRVWRLIVDENNKMRDRITTLNDGKLDKVYHQTVKKVTEDYENLHFNTAISQLMV
                     FINEAYKVDALPYEYIEGFVQLLAPIAPHMGEELWAILGNDGGISYAPWPTYDEAALV
                     EDEVEVVFQVNGKVRAKSNVPRDLGKDELEKAALANEIVQEYIEGKTVRKVIAVPNKL
                     VNIVAN"
     gene            7944..9497
                     /locus_tag="ACG842_001937"
     CDS             7944..9497
                     /locus_tag="ACG842_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WD40/YVTN/BNR-like repeat-containing protein"
                     /protein_id="HFQ0248469.1"
                     /translation="MDQELFIIEVLSAKQKIIEKAKKRAKRIMFKLLSLVFFSGLLFF
                     GAVSFLYRHMSAVVRSAEGMQKLNNLYHFSWGVGGITIVVLIVFLLLIWLRYHQLSRH
                     FDQWLSQRAQQFLHNKPTSQDDDYLYFEKTSTSQAFRIQKSDCQLLLRSLDDLPIYLG
                     ESKSKFGMNTFHIFLLENEPRWEKSQSSPGGYLTTKMSTLVTGFFLILLAGTIYYGRY
                     TQPKMSSHINAASHMTMNSTNDTHIQKTNEGLLADPKQKVNLTTQKINDLKLDTETHS
                     LYMTTDSGANWVFVPIDSQWLRFGEYTLTTGLIPEGYWMDNTYDISADFSWFIYSSGD
                     DVRLLTSRDNGKTWQTSIITQSVQPIRYRKAVFYGNDGLFICTSASGMSAETLYIYTT
                     MDGGITWIKSGSTMINQPVQNVSFVTTSLGFVSTRESLFYTNNGGRSFKESVLSIPEN
                     YQTGGIDLFQSPNEVTQISANTLETKFYLLKKGPIDAGKMFACLFRSTDNGETWQFVQ
                     QLSQVEQGD"
     gene            complement(9878..11662)
                     /locus_tag="ACG842_001938"
     CDS             complement(9878..11662)
                     /locus_tag="ACG842_001938"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0248470.1"
                     /translation="MEKKKTLNTRALIRFWKMIKPEHKYFYGLFLCSLFGNLLVVAMP
                     LIMGIGIDDLLSEIRKTGIGQLSFSDIKQALLFPVLLLIGCSVLSSITSFIQERTMAA
                     LSEKITLRVRKEITKKFKTLPMSFFDNHQVGDIISRTTTGLNQLSQVLLTGINQFFTS
                     VVTIAAAGLMLFYIESKLTLLVLLLIVGSSIVTTKIANKNKQISEQNQYELGVLNNRM
                     EEILAGNLVVKTFNQQAHAEAAIHEVNKKQYNAFKRAQFMNCAIYPAIRFINQLAFII
                     SAVFGAMLVLSGGVTIGFLQAYLQYINQISEPISTASYVINSIQSAMAAIDRIFEILD
                     ETDEIPDLPDARLLEQPKGKIAFKNVQFGYTSDKILMNQVDFSVKPKQTVAIVGPTGA
                     GKTTLVNLLMRFYELNKGKIEFDGLDITKLSRGELRRSFGMVLQNTWLFEGTVADNIA
                     YGKMDATREQIIEAAEIAQCDHFIRTLPDGYDTIISSENGTLSQGQQQLLTIARVILA
                     NPPVVILDEATSSVDTRTEALIQQAMEAVTKDRTSFVIAHRLSTIENADMILVMKNGD
                     IVEKGTHSELLQSPTLYASLYNSQFQIA"
     gene            complement(11677..13398)
                     /locus_tag="ACG842_001939"
     CDS             complement(11677..13398)
                     /locus_tag="ACG842_001939"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0248471.1"
                     /translation="MSILKTFLQKNKGLALLTFVMICLEIAGTLGVPKLVAKLIDTGI
                     ASGEASVIKKIGVEMLLVAFLGTIAAVASSYFSALASARFGFQTREKYFRKFQQLSMK
                     DISRFSSGSLLTRMTNDVDNVQQMIVLFCQMILPAPVICVFTILMMFRYSLLLTWVTL
                     ISVVFYVWIVYRLMKRGAPLSLSIQPKMDRITITLREFFTGINMIRAFDNQSYEEKRT
                     NKSFADYANQMIKVNRIFAWVTPIAFLLMGIVYASILWFGGNLVAEGALQIGVVTAVV
                     EYSMLTLAYLMIAAMVLVIIPKSFASLRRIEEILQAEIEIEEETIKTVAEPTFDQTNA
                     AAFEHVTFKYTEISDPVLEDIDFTIPKGKTTAIVGGTGSGKSSLAKLLLRLSDPTIGK
                     VTLGGIPLTQMTQEQIRSHISYVPQKAFLFSGTILSNLQMGNEYASTEELSKAIKIAQ
                     LEELVDTLPDGLDSFVAQGGSNYSGGQKQRICIARALIKPAEIYVFDDSFSALDYKTD
                     AALRAALHKHMSEKTLVIVAQRLSTIQQADNIIVLDEGKIVGQGTHEELLSNCQTYQE
                     FAKSQGI"
     gene            13498..14259
                     /locus_tag="ACG842_001940"
     CDS             13498..14259
                     /locus_tag="ACG842_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFQ0248472.1"
                     /translation="MKKMYTIGQVSELFDLPKSTIRYWDEQGLIRSSRQEENDYRLFD
                     IDDIFMLYDIVFYRKLDIPIKQMKNLYGKTLTELYETLDETERRIHKELVVMKQKQKE
                     IREQKKQLKLMIDTNEEEFPVEEIPFDCMISTEFEDIVEIKKFLPNYSSFGMMSMPAS
                     SSQTMYGFFIDPSEVHLFTQDVIWEKKDTAVYRRFLLKSEMNHAERNNILEIRERMYE
                     KGWKTGEVIGQYLLTNTDENNIRTEYYHAWIEMKK"
BASE COUNT         4745 a         2704 c         2913 g         4258 t
ORIGIN      
        1 ctgtcctgca tcaagtaata agaacagcca atcaaaaaca gcttctcatg tttttgattt
       61 ggttggactt attaccgcag atataagctt ttgagcaccg tgaataaaga ggttatgaaa
      121 tcaacgctct aacctgagta ttagagaaaa aaacgcaaaa catatcattt atgttttgcg
      181 ttttttattt atcttttttt ctcaatacaa tataaaatag gcggctggtt cttttggttg
      241 ataaaccgat aattcagcac attatatgtt tcctgtggca gtttggaaca aaaagaatca
      301 accatatcca gttctttttc tccaccttca tgaccatagt agacgacaag gatcaatcgt
      361 ccgcgaggca caagtcgttt caatatttct tccatagctg tgcgtgtagt ctctggcata
      421 gtgatgattg ctttatcact ttttgggaga taccctagat tgaaaatccc tgctttgaga
      481 ttttgttgtt cagctatcac ttctcctaac gtttcgtggc cttgtagaaa caatgagaca
      541 cgttcttcta actgacgctg ttctagcctt tcacgagtag catcgattgc ttgttgctgg
      601 atatcaaaac tatagacatg accagttttt ccgatgtgtt ctgctagaaa aaccgtatcg
      661 tggccattgc ccatcgttgc atctacgacg tgatcccctg gttgcaagat ttcttgcagt
      721 aatgtatggc tgaaatgcaa agcagtttgc aacatcagac gctcaccttt ttccgtacat
      781 catattttcc ttggaagcta tctctgcgaa gaagttcttc atcaattgca ttcaagactt
      841 cccatttttt caagctccac attggtccaa tcaaggaatc ccacggcgca tcccctgtca
      901 aacgatggat gatgatctct tttggtatca tttccagctg gtcgcaaatg acattgacat
      961 agtcaggacg gctcattaat tgaaggcgac cttcatgata gtcccgcaac attctcgttt
     1021 tgcgcatcag atgcatcaaa tggagcttga ttccttggat atctgaatct aaaatggtgc
     1081 gtctgacatt ttctttcatc atttctaaac tttcgcctgg taatccgttg atcaaatgcg
     1141 tacaaacacg gatattatgt ttgcgtaatt tagccacgcc atctaaataa gtttgataat
     1201 catgtgctcg gttgatcagt tttgaagttt cttcaaaagt tgtttgcaat ccaagttcta
     1261 cccataaata catccgctcg ttcagttcag caaggtaatc aactacttca tctggcagac
     1321 aatccggtcg tgtaccgact gataatccta cgacacctgg taaattgact acttgctcaa
     1381 atcgttcttt gattatttca actggcgcat gggtgtttgt aaaattttgg aaataaacga
     1441 tgtattgttg gacatgaggc catttttgat gcatcatgtc gatttctttc tggaattgga
     1501 ttggaagagg gtcttcaggt gcaacgatca tgtctcccga accagataca ctgcaaaaag
     1561 tacaaccgcc atgggcaaca gtcccatctc gattcggaca gtcaaatcct ccatcgatcg
     1621 gcaccttgaa aattttcccg ccaaactcat ttcgtaaagc gtaattccaa gaatgatatc
     1681 ttttatttcc ttctgaataa ggaaaagtca ttaaaagtcc ttctttctgt aagagttatc
     1741 tatttgtttt cttttttctc tatacactgg ataagtcagg cacagccaag aaagactgag
     1801 taagtaaccg ccgatgatat cagtaggaaa atgaacgccc agataaatgc ggcttatccc
     1861 gacacttact ataataaaca tcatcagcaa ttgaagcaat gtgcgtaagg ttcttttttg
     1921 aacaaaagct ggaagcagaa atagtaaaga accgtaaaga atcatactgc ctgctgcatg
     1981 tccactaggg aaactgtaac ttgtttcaac tactaagtgc gccaatgaag gacgctctcg
     2041 catgaaaaat agtttgatca ccgggttgat gattccagca attacaataa atccagaaac
     2101 gaaccaaaat gtttctgccc tttttttctt ataccacatc cataaagccg cacaagcaaa
     2161 taaaatgatg acggtaagag gattaccgaa tttagtcata aagagttggt agctgttcag
     2221 atcaggataa gaagctctga caatgcctgt aaaccactga tcgaaaccag aaagccacga
     2281 cagatggaac tttacgatcc agcccaaaat gaaaaacagg acaagaaaga agctaccagc
     2341 cacctgaaat gatgttttat atttcataca attcctcctt tgaagcaatg acttattata
     2401 gcatttttgg tccagcgaaa aaaacggtaa agagataaaa ccaatgtatg attcctcttt
     2461 gaataaccaa tattcttata aaaaaaataa ctgtaatcgg aaagacattt tttggtaatg
     2521 caaatgaaat gttagggaaa taaaatcagt gtttttcgaa atccttttaa acaagaacgt
     2581 agacgtagga agaacttgaa aatgaacaga agttgttcat tacggtaaat tacaatatga
     2641 ttacaaaaaa caacaaagct cttaaataac ttttaataaa tattaagaga agttataatt
     2701 attaataata gataaattga agttggagga tagaagatgg aaaacattgc aagaaaagaa
     2761 cggagacgtt tagaacagac caaacgtttt cgaaaagcaa aacgaggggc agcaatcgtg
     2821 ggaactgcga tggtaggctg ttctgtcgct gccccattag tacagccggt tcctgttcaa
     2881 gcggatgaaa cacctgttca gttcggcgca agaatcaata cagcggcatt tatcacagaa
     2941 attgctactt atgctcaacc aatcgctcaa gccaacgatc tatatgcatc agtgatgatc
     3001 gcacaagcag ttgtagaaag cggttggggc agtagtgcgt tatctcaagc accttattat
     3061 aatctttttg ggataaaagg cagctatcaa ggacaaaccg tctacatgga tacgcttgaa
     3121 tttttaaata atcaatgggt aaataaaaag gagcctttcc gacaatatcc ttcttttgct
     3181 gaatctttca atgacaatgc ctatgtactt agaaatactt catttggcaa cggttattac
     3241 tatgccggta cttggaagag caacacgaaa tcctatacag atgcgacagc ttgtttgaca
     3301 gggcgttatg cgactgatcc tggttacgca ggcaaattaa ataatatcat cgcaacttac
     3361 ggattgaccc aatatgatac accagctagc ggaaacgcag gcggtggagc gactacaggt
     3421 ggcaataata ctggaagtgc aggtagtact ggcggttcaa gcaattctgg taatacaaac
     3481 tcaggttcag caacttcgac cacttatact gtccaatctg gtgattctgt ttggggaatc
     3541 tcaaataaat tcggtatcac aatggatcaa ttgatccaat ggaacaacat ccaaaacaat
     3601 ttcatttatc caggacaaaa actgacaatc aaaggtagcc aggaaaatgg ctcttcgacg
     3661 aataattccg gtaacaatac caattcttca ggaaatgctg gtacatcaaa cggaggacaa
     3721 acaactggtg cgaaatacac tgttcaatct ggtgattctg tttggaagat ctcaaatgat
     3781 cacggaatta cgatggctca attgattgaa tggaacaata tcaagaacaa tttcgtttat
     3841 ccaggacaac agctaatcgt atcaaatggc ggttcttcta atgctggagc aaatactggc
     3901 tcaaatactg ggaatacaag tggctctagt tcgtccagct caagttcttc taatacagct
     3961 tcaggaacga aatatacagt taaagccgga gactctgttt ggagcgtctc aaataaatac
     4021 ggaattacga tggctcagct aatccaatgg aacaatatcc aaaataactt tatctaccca
     4081 ggacaacaac tgactgtttc aaacagtgga tcaacaaaca gcacgtcagg taatacaggt
     4141 tcgtcaacca atacaaataa cagcagttca gttaatactg gcagcagttc gtctcaaggc
     4201 acttataccg tgaaagcagg tgaatctgtt tggagcgtgg caaataaaaa cggcatcacg
     4261 atggatcagt taatcgaatg gaataatatc aaaaacaact tcatttatcc aggacagcaa
     4321 ttgatcgtta aaggtggaac ttcggcaaat acaaatacag gttcaactac ttctgcagcg
     4381 aaaccaaata caccgaatac gtctgctacg acaagtacat catccactgg taatacgatg
     4441 tataccgtga aagcgggcga gtcagtttgg agcgtagcga acaaacatca tatcacgatg
     4501 gatcagttaa tcgaatggaa taatatcaaa aataatttca tctatccagg acagcaagtg
     4561 attgtgaaga aaggttcatc tcaatcaaca aaccaacaat cagttactgg ttcgaaaacc
     4621 tatactgtga aatctggaga atctgtttgg agtgttgctg acagtcatgg aattacaatg
     4681 gctcaactaa tcgaatggaa caatattaag aacaacttta tttatccagg tcaaacgttg
     4741 attgtagcta aataaaatag ctgttcgaag aaataacgat tgaattccac aaaaatattt
     4801 gttactataa ggctacttaa atatgacaac acttgaaaag gaagtagtaa aaagactgaa
     4861 gtttcagaga gcgcatggct ggtgagaatg tgcacggatg cttttgaact cgcctttgag
     4921 ttgcccttgc tgaatacccg agtaagcaag cggcggtgac ggccgttaca acgttcgagg
     4981 ccttttcact aaggtaaaat taggtggtac cgcgtagatt acgtcctata aaaagagcaa
     5041 tctttttata ggattttttt atttcttttg tgaagtttga gaaaacaaag caaaaggaaa
     5101 taaaaataaa cagattctgc cggttttttc gttatattta taatagaaac aagtgttttc
     5161 aatagaacag gaggacagca aatgagttac aatcataaag aaattgagaa aaaatggcaa
     5221 aaatactggg caaaaacgaa tactttcaac acccatgatg atcctgaaaa gccaaaattt
     5281 tacgcactgg atatgtttcc ctatccatcc ggtcaagggc tacatgtagg gcatcctgaa
     5341 ggttatacgg caacggatat cctttcacgt gtgaaaagaa gtcaaggata caacgtgctg
     5401 catccaatgg ggtgggatgc attcggtctg cctgctgaac aatatgcgct agatacagga
     5461 aacgatccag ctgagtttac ccaaaaaaat atcgaaacat tccgacgcca aatcaattct
     5521 ttaggattta gttatgactg gaatcgcgaa gtgaatacaa ctgatccgga gtattataaa
     5581 tggacacaat ggatctttac gaagctttat gaaaaaggtc tagcttatga agcagaagta
     5641 gcggtaaact gggtacctga attaggaaca gttatttcaa atgaagaagt gatcgatggg
     5701 aaaagtgaac gtggtggcta tgatgtagtc agaaaaccaa tgcgccaatg gatgctgaaa
     5761 atcactgctt atgctgatcg cttattggat gatctagaat tagttgattg gccagaaagc
     5821 attaaagaaa tgcaaagaaa ttggatcggt cgttcagtag gagcaaatat tgaatttaaa
     5881 gtggctggta cggataaatc ctataccgta tttacgacac gaccagatac tctattcggt
     5941 gcaacttact cggttcttgc acctgaacta gatcttgtta gagaaattac gacccctgaa
     6001 caaaaagctg ctgtagaagc ttatatcgaa gagacagcta aaaaatcaga tctaaagaga
     6061 acggatcttg caaaagaaaa aacaggagta tttactggtg cttatgcaat caatccagtt
     6121 aatggcaagg aaatcccaat ctggatcgcc gattatgtat tatcttcata cggcacagga
     6181 gccatcatgg ctgttccagc acatgatgaa cgtgactttg aattcgctca aactttcgga
     6241 ctagaaattc ttccagttat cgaaggtgga gatgttgaaa aagctgctta cacagaagat
     6301 ggtacgcaca tcaactctga atttctagac ggcatgaaca aacaagaagc aatcgataaa
     6361 atgaatgaat ggctagaaga aaatggcgta gggaaaaaag aagtcagcta tcgcttacgt
     6421 gactggctat tctcccgtca acgttactgg ggtgaaccaa tcccaatcat ccattgggag
     6481 gacggaacag tcacagctgt cccagaagaa gagctaccat tacggttgcc taagacaaaa
     6541 aacatcaagc caagcggtac tggagaatct ccattagcaa atatcgagga gtgggtcaat
     6601 gtcgttgatc ctgtaacagg taaaaaaggc cgtcgcgaaa cgaatacaat gccacaatgg
     6661 gcaggaagct catggtatta cctgcgttat atcgaccctc ataacaaaaa agaactagca
     6721 gattatgaaa agttgaagcg ttggctacca gttgacatct acattggtgg agcagaacat
     6781 gcggtacttc atttactata tgcacgattc tggcataaat tcttatacga tatcggtgtt
     6841 gttcctacaa aagaaccatt ccaaaaacta tacaatcaag gaatgatctt aggcgaaaac
     6901 aacgaaaaaa tgtcaaaatc tcgtggtaat gtagtcaatc ctgatgatgt ggtcgaaact
     6961 tacggtgcag atacactgcg catgtacgaa atgttcatgg ggccattaga tgcttcgatt
     7021 gcttggagcg aaaatggctt agaaggaagc cgtaaattct tggatcgtgt atggcgtttg
     7081 atcgttgatg agaacaataa aatgcgtgac cgtatcacta cattgaatga tggcaagctt
     7141 gataaagtgt atcatcaaac agtgaaaaaa gtgacagaag actatgaaaa tcttcatttc
     7201 aatacagcaa tctcccaatt gatggtcttt atcaatgaag cctataaagt ggacgctctg
     7261 ccttatgaat atatcgaagg atttgttcaa ctactcgcac cgattgctcc tcacatgggt
     7321 gaagaactat gggcaatcct tggaaatgat ggtggtattt cttacgcacc atggccaaca
     7381 tacgatgaag cagcacttgt tgaagacgaa gtagaagttg ttttccaagt aaacggtaaa
     7441 gttcgtgcga aaagcaatgt tccccgagac ttaggaaaag atgagctaga aaaagcagca
     7501 ttagccaatg aaatagtgca agaatatatt gaaggcaaaa cagtcagaaa agttattgcg
     7561 gtaccaaaca agttagtcaa tatcgttgct aattaattgg ctctttgtca ataaggactg
     7621 atgagttgtg caaaatcaaa tctgggccag aatgaacttc attctgtctc agattttttg
     7681 ttatttcaaa ttgattaatt gattgatcaa aaaggttttg attttcatgt tctcaaacac
     7741 gttcaatgtc tttttcttct gaatggtctt gtaagagatc tctgtaatca tcaaattttt
     7801 gtctattaat cttagtattt tttgatgtat aaactacctg aaattgagag tacataccaa
     7861 attcataaat tttttattag tgggactagt gattgtatgg attttgacgc ataatgaatg
     7921 aagtatgaga aaaggagaga caaatggatc aagaactatt tattatcgaa gtattatcag
     7981 ctaaacaaaa aattatcgag aaagcgaaaa aaagagcgaa aagaataatg ttcaagcttt
     8041 taagcttagt atttttctct ggtttgcttt ttttcggagc tgtatcgttc ctatatcgcc
     8101 atatgagtgc agtagtccgt tcagctgaag ggatgcaaaa attaaataat ttgtatcact
     8161 tttcgtgggg agttggcgga ataacaattg ttgtgcttat tgtttttctt ctacttattt
     8221 ggctccgtta ccatcaattg tcgcgtcatt tcgatcaatg gcttagtcaa agagcacaac
     8281 agtttttaca taataaacca acttctcaag atgatgacta tctttatttt gagaagactt
     8341 cgacgagtca agcttttcgg atacaaaaaa gtgattgcca gttattgctg agatcacttg
     8401 atgatttacc tatctactta ggcgaaagca aatcaaaatt cggaatgaac acattccata
     8461 tttttttact agaaaacgaa cccagatggg aaaaaagcca gtcttcgcct ggtggatacc
     8521 tgacaactaa gatgagtaca ttagtcactg gttttttcct cattctttta gctggaacaa
     8581 tctattatgg gagatacact cagccaaaaa tgtcttctca tataaatgct gcttctcaca
     8641 tgacaatgaa ctcaactaac gatacgcata tacaaaagac aaatgaaggg ttgttagctg
     8701 atcctaagca aaaggtaaat ttgacaacac aaaagataaa tgatctcaaa cttgatacag
     8761 agacgcactc tctctatatg acaacagata gtggtgctaa ttgggtattt gtgccgattg
     8821 attcgcaatg gttgcgtttt ggcgaataca ccctgacaac ggggctgatt cctgaaggtt
     8881 attggatgga caacacatat gatatttccg ccgatttctc atggtttatc tattcttctg
     8941 gggatgatgt acgtctgctg acttcaagag ataatggtaa aacatggcaa acgtctatta
     9001 tcacccaatc agttcagccg atacgttatc gaaaagcggt attttatggc aacgatggtc
     9061 tatttatctg tacatcagcc agtgggatgt ctgcagaaac tttgtacatt tacactacta
     9121 tggatggcgg aatcacttgg ataaaaagtg gaagtacaat gatcaatcag ccagtacaaa
     9181 atgtcagctt cgttacaact tctttaggtt ttgtttcgac acgtgaaagt ttgttttata
     9241 caaacaacgg aggacgctca ttcaaagaat cagttctgtc gattccggaa aactatcaga
     9301 caggggggat agatcttttt caatcaccaa atgaagttac tcaaataagc gcgaatacgt
     9361 tagagacaaa attttatttg ttgaaaaaag gaccaatcga tgcagggaaa atgtttgcct
     9421 gcctcttccg ttcaactgat aacggggaaa catggcaatt cgttcagcaa ctatcgcaag
     9481 tagaacaagg agattaaaga agagagattg tgagcctgac gttcagcacc gagtcacaag
     9541 aaacaaattc aaaaaacagc ttctcatatt tgttgaatag tgtaactcgt gattcggagt
     9601 tgacaagagc aacaagaagc gattttgaag tatagcttct cgtattttac aagatcgtgt
     9661 cttattgccg ataggttgtc tcttgaacac cgtttattcg taaaaaggga ttgtgacaag
     9721 tcttttgtca ctacttaccg aacgaaactg aaggagctat ccaaaaagta tcattagtat
     9781 tattctttgt gtttctagtg gcaccactag tacatttaaa aaagctatcg atcatggact
     9841 aatcatacat gatcgatagc tttttagata atgtttgtta cgctatctga aattgactat
     9901 tataaagact ggcataaagc gttggtgatt ggagtagttc agaatgtgtt cctttttcga
     9961 cgatatcgcc attcttcatt acaaggatca tgtctgcatt ttcaatcgtt gacaaacggt
    10021 gtgcaatcac aaaacttgtc ctatcttttg tcacagcttc cattgcttgc tgaatcaatg
    10081 cttctgttcg tgtatctacg ctagaagttg cttcatctaa aataacgaca ggaggattcg
    10141 ctagaatgac tcgagcaatt gtcaataatt gttgctgtcc ttgagataac gtgccgtttt
    10201 cgctagaaat gatcgtatca tatccgtctg gcagtgttcg aataaagtga tcacattgtg
    10261 cgatttcagc cgcttcgata atttgttctc ttgtggcatc cattttcccg taggcgatat
    10321 tatcagctac cgttccttca aacagccagg tattttgtaa aaccatccca aaagaacgac
    10381 gtagttctcc acgtgaaagc ttggtaatat caagaccgtc gaattcgatc tttcctttat
    10441 tcagttcata aaaacgcatc agcagattga ctaaagtcgt ttttcctgca ccagttggac
    10501 caacgatcgc taccgtttgt tttggcttta ctgaaaaatc gacttgattc atcaatattt
    10561 tatctgatgt gtaaccaaac tgtacatttt tgaaagctat ttttcctttc ggttgttcta
    10621 aaagtcttgc atctggaagg tctggaattt catctgtttc atctaatatc tcaaatatcc
    10681 tatcgattgc tgccattgcg gattggatcg agttgatcac ataagaagcg gtagaaattg
    10741 gttcagaaat ctgattgatg tactgaagat aagcttgtaa gaagccgatc gtcacaccac
    10801 ctgatagaac aagcatagca ccgaataccg cacttatgat gaaggcgagt tgattaatga
    10861 aacgaatggc cggatagata gcacagttca taaattgagc acgtttaaaa gcgttgtatt
    10921 gctttttatt tacttcgtgt atagcagctt ctgcatgagc ttgctgattg aatgttttga
    10981 ccactagatt accagctaag atttcttcca ttcgattatt taaaacaccc agttcatact
    11041 gattctgttc agaaatctgt ttgtttttgt tcgcaatctt agttgtaaca attgaactgc
    11101 caacgatcag caataagacg agcaaagtaa gcttgctttc gatataaaac agcatcagac
    11161 cagcagcagc tattgttacg acagaagtaa aaaattgatt gatacctgtc aataacactt
    11221 gagaaagttg attgagaccg gtagttgtcc gactaatgat gtcacctact tgatgattgt
    11281 caaaaaacga catcggtaat gttttgaatt tttttgtgat ttcttttcgc accctcaatg
    11341 tgatcttttc gcttaacgct gccattgtcc gttcctgaat aaatgaagtg atgctgctta
    11401 agactgaaca gccgatcagc aataagacag ggaacaacaa tgcttgtttg atatcactga
    11461 aagaaagctg tccaatacct gtttttctta tttctgataa gagatcatcg atcccgattc
    11521 ccatgatcaa gggcatggct acgactaata ggttcccgaa taaactgcat agaaacaatc
    11581 cgtaaaaata tttatgttca ggtttgatca ttttccaaaa acgtatcaaa gcacgcgtgt
    11641 tcaacgtttt tttcttttcc atcgtatccc ttctttctat attccttgtg attttgcaaa
    11701 ctcctgataa gtttggcaat tagacagtag ttcttcatga gtaccttgtc caacgatttt
    11761 tccttcatct aaaacaatga tgttatctgc ttgttggatt gtactgaggc gttgagcaac
    11821 aatcacgaga gttttctccg acatgtgttt atgaagggct gctcttaatg ctgcatctgt
    11881 tttatagtct aaagcggaga aactatcatc gaatacatag atctctgctg gtttgatcaa
    11941 cgctcgagca atacatatcc gctgtttctg gccccctgaa taattagaac cgccttgagc
    12001 aacaaagctg tccaatccgt caggaagggt gtccactagt tcttctaatt gagcgatctt
    12061 gatcgcttta gacaactctt ctgttgaggc atattcattt cccatctgca agttgcttaa
    12121 aattgtcccg ctgaataaga aagccttctg agggacataa ctgatgtggg agcggatctg
    12181 ttcttgagtc atttgagtaa gaggaatacc accaagggtt acttttccta tcgtagggtc
    12241 actaagtctc aagagaagtt tcgccaaact actttttcca gatccggtcc cgccaacgat
    12301 cgctgttgtt tttcctttag gtattgtgaa atcaatatct tctagaactg gatcagagat
    12361 ttcagtatat ttaaatgtga catgttcaaa agcagctgca tttgtctgat caaaggttgg
    12421 ttcagctact gttttgatcg tctcttcctc aatttcgatc tctgcttgca gtatttcttc
    12481 tatccgtcgt aatgaggcaa acgatttagg gataatgacc aataccatag ctgcgatcat
    12541 caagtaagca agtgtcagca tagagtattc gactacagca gtcacgacac cgatttgtaa
    12601 tgctccttct gctacaagat ttccgccaaa ccacaaaata gaagcataaa cgattcccat
    12661 gagcaaaaaa gcaattggag tgacccaagc aaatattcgg ttcactttga tcatttgatt
    12721 agcatagtca gcaaagctct tattcgttcg cttttcttca tatgattgat tatcaaaggc
    12781 acggatcata ttaatcccag taaagaattc tctcaatgtg atggtgatcc gatccatttt
    12841 tggctggata ctcaaagata aaggtgcccc tcgtttcatc agtcgataaa caatccatac
    12901 ataaaaaaca acagagatca atgtaaccca tgttagtaat agagaataac gaaacatcat
    12961 gagaatggtg aaaacgcaaa taaccggtgc tggtaagatc atttggcaaa ataaaacaat
    13021 catttgctgg acattatcga catcattcgt cattcttgtc aaaagagatc cagaactaaa
    13081 acgactgata tccttcattg acagctgttg gaatttacgg aaatatttct cccgcgtttg
    13141 gaatccaaac ctagcggaag ccaatgctga aaagtaacta gaggcaactg cagcgattgt
    13201 tcctagaaac gctacaagca gcatttcaac tccaattttt tttataactg aggcttcgcc
    13261 acttgctata cctgtgtcga tcagctttgc tactaatttt ggtacaccta atgtaccggc
    13321 gatttctaaa cagatcatca caaaggtaag gagagcgagt cctttgtttt tctgtaagaa
    13381 tgtttttaag atagacatat tattcctcct tttttcctga tacttttcga ttctatacct
    13441 tcaagtaact tgaaggtcaa tagaaaaaag aataaactga aaaagaggtg agaaaaaatg
    13501 aaaaaaatgt atacgattgg ccaagtatcg gaactatttg atttgccaaa atcaacgatc
    13561 cgctattggg atgaacaagg attaatccgt tcaagtagac aagaagagaa tgattatcga
    13621 ttgtttgata tcgatgatat ttttatgctt tatgatatcg ttttttatag aaaattagat
    13681 ataccgatca aacagatgaa gaatttatat ggaaaaacat tgacggagtt atatgagaca
    13741 cttgatgaaa cagaaagaag aatccataaa gaactggtgg tgatgaaaca aaaacaaaaa
    13801 gagataaggg agcaaaaaaa acagttgaaa ttgatgatcg atacaaacga ggaagaattt
    13861 cctgtagaag agatcccttt tgactgtatg atctcaacgg agtttgaaga tattgtggaa
    13921 atcaaaaaat ttctacctaa ctattcaagc tttgggatga tgagtatgcc agcttccagt
    13981 tcacagacga tgtatggatt ttttatcgac cctagtgaag tacatttatt tactcaagac
    14041 gtgatttggg agaaaaaaga tacggctgtt tatcgtcgat tcttattgaa atccgagatg
    14101 aaccatgcgg aacgtaataa tattttagaa atcagagaaa gaatgtatga aaaaggatgg
    14161 aaaacaggag aagtgatcgg ccagtatcta ttgacgaata cggatgaaaa caatatcaga
    14221 actgaatatt atcatgcatg gatagaaatg aaaaaatagg aaatatgtgt ttaagataac
    14281 tcgaaaatga tggttgtttt agttataaag aaaaattttc tggttcgatt acgggtttaa
    14341 tgtgggggat tattagtcgt ggatagatag aattttagtt ttttagagga aatatgtctt
    14401 taaaaatgga atggttgcaa ttcttagcga tatatacgat gtaattgtcc tcttaaaaaa
    14461 gtatggaaaa ataattgtat tgggtgttat gtataattca tggtgaactg agactaatga
    14521 gaagaaaaat tcatattgtt atgagtgaga ttgtatcaga tgagaatgat aaattaaata
    14581 ggtgcataaa agcattacac ttaaaataca ttgttgctta
//
LOCUS       DAZKLM010000067        14548 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3764
            SAMEA115706913-rid22582563.denovo.069, whole genome shotgun
            sequence.
ACCESSION   DAZKLM010000067 DAZKLM010000000
VERSION     DAZKLM010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706913
            Sequence Read Archive: ERR13196647
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14548)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14548)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287823.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287823.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,004
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,849
            CDSs (with protein)               :: 2,849
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 41 of 96
            Pseudo Genes (incomplete)         :: 52 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 15 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14548
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706913-rid22582563.denovo.069"
                     /isolate="VRE3764"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(164..388)
                     /locus_tag="ACG842_001941"
     CDS             complement(164..388)
                     /locus_tag="ACG842_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein"
                     /protein_id="HFQ0248473.1"
                     /translation="METEWQRRERKKQKKEEMLRLCRLLDVKDWDKNEQIAQKVRSII
                     HKEEKVKNKDTRRTENEKRKVQKKREKSKS"
     gene            complement(586..2166)
                     /locus_tag="ACG842_001942"
     CDS             complement(586..2166)
                     /locus_tag="ACG842_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HFQ0248474.1"
                     /translation="MNNPKLKQQVDNRRTFAIISHPDAGKTTITEQLLLFGGAIRQAG
                     TVKGKKTGNFAKSDWMEIEKQRGISVTSSVMQFDYQGKRVNILDTPGHEDFSEDTYRT
                     LMAVDSAVMVIDSAKGIEAQTKKLFQVVKKRGIPIFTFINKLDRDGREPLELLEELEE
                     LLDIESYPMNWPIGMGKGLEGLYDIYHNRVEFYRPENYQDERLVELDEDGLLPENHPL
                     TENSLYEQVLEEVELIKEAGDAFNQEKIARGDQTPVFFGSALTNFGVQTFLETFVELA
                     PAPYGHKTQDDQMVSPYEEEFSGFVFKIQANMNPAHRDRIAFVRICSGTFERGMDVWL
                     ERTNKKLKLSNVTQFMADSRENVEKAVAGDIIGVYDTGNYQIGDTLFEGKLKVAYEEL
                     PSFTPELFMKVTAKNVMKQKSFHKGIYQLVQEGAIQLYKTYLTEEYIIGAVGQLQFEV
                     FQYRMLNEYNAEVIMSPMGHKIARWIDPEDLDEKMSSSRNILARDRFDQPLFLFENQF
                     AERWFADKYPNVKLKSLM"
     gene            complement(2567..3943)
                     /locus_tag="ACG842_001943"
     CDS             complement(2567..3943)
                     /locus_tag="ACG842_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016610209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HFQ0248475.1"
                     /translation="MNADPESQSLLAQILLLIVLTLINAFLAASEIAVVSINKNRIEQ
                     KADEGDVKSQKLLKILQNPNNFLSTIQVGITLVNILSGASLANTLSERLAPVLGGGTA
                     AKNIANIIVLAILTYVSIVFGELYPKRIALNKSEEVAKFTSGLIRLIGVVAKPFVWLL
                     SASTSLLARITPMTFDDEDSKMTRDEMRYMLENEGVLNNEELEMLQGVFSLDTKVARE
                     VMVPRTDAFMIDINDTVEENVNEVLSENYSRIPVYNEDKDKVVGILHTKNLLKAAHKF
                     GFDNLDIKKIMQEPLFVPETVFIDDLLYEMKKTQNQMAILLDEYGGVVGLATLEDLLE
                     EIVGEIDDESDEVENLYEKIDEHEYIIQGRMLIDEFNEAFDSDLHMSDVDTMAGYLIT
                     ALGMIPDEGEKLSFDVDNITLVSEEMEGSRILKIRVIFHDPEETEAEPEEERRYFRKE
                     FEDDEPRR"
     gene            complement(4071..5159)
                     /locus_tag="ACG842_001944"
     CDS             complement(4071..5159)
                     /locus_tag="ACG842_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFQ0248476.1"
                     /translation="MFWSLELLIIAALIMISSEINFIFEPIGTFFSTLFAPVLIAGFL
                     YYLLNPIVKLLMKAKIKRIWAVAIVLLLLVAAIIWILLSVIPSLVQQISSLASNMPDF
                     IKQVESWLKEVAELPLFKEVDINKYFEQLDISYGTIIQQFLSGVSNSLGSIVGTIASA
                     TVVIITAPFILFYMLKDGEKLVPNIQQFFPEKRRNQIVELLGQLNKTLANYISGQAIE
                     CLFVGTFTFLGYMVIGVDYAFLFGVIAGLTNLIPYLGPYLGLAPAFLVTVFNDPVKAL
                     LCCVVVLIVQQLDGNIIYPNVIGKSLKIHPLTIIIVLLVAGNIAGLLGIFLGVPFYAI
                     CKTIISYIIKIVKEDKQNENKKKIATTS"
     gene            5331..5765
                     /locus_tag="ACG842_001945"
     CDS             5331..5765
                     /locus_tag="ACG842_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0248477.1"
                     /translation="MKVVQTKDTMSNIYLDAVRIRHQVFVVEQGVPLSREIDKDEAHC
                     IHFVLYSDKKEPQGTVRLLPLENGKMKLQRMAILSEYRHQGLGKILIEEAENFAKNQG
                     YNTILLGAQSTAETFYEKLGYTAYGDPFEDAGMPHIYMKKTL"
     gene            complement(5843..6376)
                     /locus_tag="ACG842_001946"
     CDS             complement(5843..6376)
                     /locus_tag="ACG842_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HFQ0248478.1"
                     /translation="MGVPKEGEFVTIQSYKHDGKLHRTWRDTMVLKTSEHSMIGVNDH
                     TLVTESDGRRWVTREPAIVYFHKKYWFNVIAMIREKGVSYYCNLASPFLLDDEALKYI
                     DYDLDIKVFPDGEKRLLDVDEYEMHSKMMNYPNDIDFILKENVKILVDWINNGDGPFS
                     EGYIDIWYNRYKQLSRK"
     gene            complement(6470..7648)
                     /gene="mutY"
                     /locus_tag="ACG842_001947"
     CDS             complement(6470..7648)
                     /gene="mutY"
                     /locus_tag="ACG842_001947"
                     /EC_number="3.2.2.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HFQ0248479.1"
                     /translation="MNKEWEKWTDKETKEFQDQFIQWYEQEKRNLPWRYNRDPYRIWI
                     SEIMLQQTRVDTVIDYFYRFMEWFPTIEELANAPEEKLLKAWEGLGYYSRARNIQAAA
                     KQIMSEFDGEMPQTPEEISSLKGIGPYTTGAIASIAFGLPEPAVDGNVMRVVSRLFCI
                     EADIAKASSRKIFDEAMRKIIDEKHPGEFNQAMMDLGSAICTPTSPKCETCPIQAFCL
                     ANKRGIQTSFPVKTKKAKPKDVYYISAALQNHSGAYYFEERDSQKLLANMWTFPMMEV
                     TQEEYERLKKEWETKPEIDLFDDLVAEEEQNLPFEKQELFVWQTRHLGEVTHIFSHLK
                     WHVLLFYGRATEGAEQEFTENKTSKWLKPDAFDSVVFPKVQMKLVDQLEKNRNNRNPF
                     "
     gene            complement(7641..8438)
                     /gene="recX"
                     /locus_tag="ACG842_001948"
     CDS             complement(7641..8438)
                     /gene="recX"
                     /locus_tag="ACG842_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HFQ0248480.1"
                     /translation="MLTVTRISKGKGPFYRVDLSEGEALQVSEDVLVRYRLLKGRELD
                     EETIQEIKKSSGADFGFHLAMNYLSYQLRSEKEIRTYLKEKEILQEDRNKIVARLKEL
                     DLVDDLVYGESYVRTNMRLSDKGPKKLAQQMQQKGLKPEIIEQALSQYTFEDQVQNAH
                     QTAEKAFGKNHGKSQKELLRKIQQTLMTKGFSQEVIQQAIADLPQEADQEIEYDYLVK
                     QGDKLWRRNLRFEPKKRNLKVKQSLYQKGFDLDMIQRFITEKEEESE"
     gene            8545..9927
                     /gene="rlmD"
                     /locus_tag="ACG842_001949"
     CDS             8545..9927
                     /gene="rlmD"
                     /locus_tag="ACG842_001949"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HFQ0248481.1"
                     /translation="MAETIVKTGQTVPLKIKRLGINGEGIGYFKRLIIFVPYALPKEE
                     VLVKITKATPRYAEGELIKVKKTSKDRVVAPCPVYYECGGCQLQHLAYQAQLDFKKDL
                     LLQALEKFKPAGFRSYQLLPTIGMDEPWHYRNKAQFQLRKNKQTQKIEAGLYEANSHQ
                     LVPITDCLVQEPRTQKVMNTVVQLLNKFDAPIYDERTNSGIFRTIMVRIGVKTGELQV
                     VFITRSKKFPQKNAMIREINERLPEVVSIMQNVQAQKTSLVMGDETIHLWGKEAIEEQ
                     INEVTFDLSPRAFFQLNPQQTELLYGEGIKALDVQPNETIVDAYCGVGTIGLSVAKQA
                     KEVRGMDTVPQAIADAKKNAERLGYHNTRYEVGTAEQVLPKWLNEGFQPDGIIVDPPR
                     TGLDNALIQSLLKYPPKKLVYISCNVSTLARDLVNLSKKFKIEYLQSVDMFPQTARCE
                     VVVKMTRLAD"
     gene            complement(10101..10679)
                     /locus_tag="ACG842_001950"
     CDS             complement(10101..10679)
                     /locus_tag="ACG842_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic polysaccharide monooxygenase"
                     /protein_id="HFQ0248482.1"
                     /translation="MKKISTLLFIGLVSLGLFGFSIPGFAHGYITSPGSRAYLGTSAA
                     GNLNQNVGRAQWEPQSIEATKNTFIDGKIASAGVSGFEPLDEQTSNRWHKNMVNPGTL
                     NITWNLTAQHRTSTWDYYITKPTWNPNQPLKFSDFELITKIDDKATVPPKTVNQTITL
                     PQDRKGYNVILAVWNISDTTNAFYQVIDVNIQ"
     gene            11001..11471
                     /locus_tag="ACG842_001951"
     CDS             11001..11471
                     /locus_tag="ACG842_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TspO/MBR family protein"
                     /protein_id="HFQ0248483.1"
                     /translation="MKMIKDIRFWVCVIGIVILGFLSGLLSGNPGKYYYSLQLPPFAP
                     PSWIFGPMWTLLYILMGISLYLLLNHNNKKQRNNLVGLFVIQFIFNFIWSALFFNLRN
                     IFIAAIDITLLVIFLSVLMYQLWLHHRLAMWLMIPYYLWVLFATLLNYSIYFLN"
     gene            complement(11579..12805)
                     /locus_tag="ACG842_001952"
     CDS             complement(11579..12805)
                     /locus_tag="ACG842_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HFQ0248484.1"
                     /translation="MKRWQKVLLTLLGVFVLLIAGLSAYGIRFMGEASQTVNKISKNS
                     NRISSKRSERVSIDDKEPFSVLLLGLDTGGLGRTEQGRSDTMMVVTVNPKQKKSTIIS
                     LDRDIYTNIVGYGTVDKLNHAYAFGGVEMAMDSIEQLLDIPIDHYVTINLDGMEDLIN
                     AVGGIKVNNKIDFTLDGVHVPVGEQTLNGEKGLAYSRMRHEDPEGDIGRQRRQREVVT
                     KIVNKVLSLDGVSNYRKILKAVENNVTTDLDWDDMLDVATNYTPAFETIKQDQLQGEG
                     QMINDIYYQILGKNDLLSIQNELKKQLNIKTSDTLPNLKNENASMMFFDDSEDGDSTN
                     GDGSNSEGYTDYSSYSENTYDQYQDPNTYYQQTEDTSQYYTPSQDTQSYDPYAGQEQN
                     GYGYDQNNEYGTTSGY"
     gene            complement(13559..13759)
                     /locus_tag="ACG842_001953"
     CDS             complement(13559..13759)
                     /locus_tag="ACG842_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003230776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFQ0248485.1"
                     /translation="MEHGTVKWFNNEKGFGFITVDGGDDVFVHFSAIQGDGFKSLEEG
                     QEVEFTIVEGARGPQAAEVTKL"
     gene            complement(13893..14501)
                     /locus_tag="ACG842_001954"
     CDS             complement(13893..14501)
                     /locus_tag="ACG842_001954"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HFQ0248486.1"
                     /translation="MTYTLPDLPYAYDALEPYIDEETMHLHHDKHHNTYVTNLNSAIE
                     KYPELGEKTIEELLSDMDAIPTDIKTAVRNNGGGHANHSFFWEIMAPNAGGEPTGEIK
                     EAINEAFGDFSSFKEEFKKAAAGRFGSGWAWLVMENGKLAITSTANQDSPLMEGKTPI
                     LGLDVWEHAYYLKYKNVRPDYIAAFWNVINWDEVNKRFEAAK"
BASE COUNT         4275 a         2941 c         2408 g         4924 t
ORIGIN      
        1 taaagaacac ttttgtctca cgctctaaac acgtataaac ggcgaaagca gaagcaactc
       61 cttcggaaat aagctgaaat tcacgaaaat tgcttttcaa atttttgtga attccagctt
      121 atttcttgga gttaaacgct tctgtctcag cctctttttc tttctagctt tttgattttt
      181 ctcttttctt ttgtactttt ctcttttcat tttcagttct acgcgtgtct ttatttttaa
      241 ctttttcctc tttatggatg attgatcgta ctttttgtgc aatttgctcg tttttgtccc
      301 agtcttttac atctaataat cgacataatc gtaacatctc ttcttttttc tgtttttttc
      361 tttcacgtct ttgccattct gtttccatgt gtcacactct cctttcttta atcatatgta
      421 cgcaaaataa tttttatatt tttttgatta gacatttcta tgattttaat atagtaaaaa
      481 gagccgtcac ggaaaatccg tcacggctct aaacaaaata tgagaggctg tttttgtttg
      541 gctattctta ttacttgatg cagggcagct tgtgtcaaga cttctttaca tcaaactttt
      601 taattttaca ttgggatatt tatccgcaaa ccagcgttct gcaaattggt tctcgaataa
      661 gaacaacggt tggtcgaaac ggtcacgagc caagatattg cgacttgaac tcatcttctc
      721 atccaaatct tctggatcga tccagcgagc gattttgtgt cccattggag acatgattac
      781 ttcggcgtta tactcattta acatacgata ttggaatact tcgaattgta gctgaccaac
      841 agctccaatg atatattctt ccgttagata tgttttataa agctgaatcg ctccttcttg
      901 aactaattga tagattcctt tgtggaagga tttttgtttc atgacatttt ttgcagtcac
      961 tttcataaac agctctggag taaatgaagg gagttcttca taagcaactt ttagttttcc
     1021 ttcgaacaat gtatccccaa tctgataatt gcctgtatca tatacaccga tgatatcgcc
     1081 tgctactgct ttttcgacgt tttctctcga atctgccata aattgtgtga cattgcttaa
     1141 tttcagtttt ttatttgtac gttccaacca gacatccatt cctcgttcga atgttccaga
     1201 acagattcgg acaaaagcga ttcggtcacg atgggctgga ttcatatttg cttggatctt
     1261 gaatacgaaa cctgaaaatt cttcctcata aggacttacc atctgatcat cttgtgtttt
     1321 gtgtccataa ggagctggtg ccaattcaac gaaagtctct aaaaaagtct gtacaccgaa
     1381 gttagtcaat gcagaaccaa aaaatactgg tgtctgatcg ccacgagcaa ttttttcttg
     1441 attaaatgca tctccagcct ctttgattag ttctacttct tctaagactt gttcatataa
     1501 ggaattttca gttagaggat gattttcggg aagcagtcca tcttcatcca attctactaa
     1561 tcgttcatcc tgataatttt ccggacgata aaactctaca cgattatggt agatatcata
     1621 aagaccttct agccctttac ccataccgat tggccaattc atcggataag attcgatatc
     1681 taatagctct tctaattctt ctaacaattc caatggttca cgaccatcac gatctagttt
     1741 gttgataaaa gtaaatatcg ggatgccacg tttttttaca acttggaata atttctttgt
     1801 ctgtgcttcg atcccttttg cactatcgat taccatgacc gcactatcca cggccatcaa
     1861 agtacgataa gtatcttcag aaaaatcttc atgtcctggt gtatccaaga tatttactcg
     1921 tttcccttga taatcaaact gcataacaga actagtaaca gaaatcccac gttgtttctc
     1981 gatttccatc cagtctgatt tggcaaaatt acctgttttt ttgcctttta cggtcccggc
     2041 ttgacggata gccccaccaa acaatagtaa ttgttctgta atcgttgttt tcccagcatc
     2101 cggatgggaa atgatcgcaa aagttctgcg attgtccact tgttgcttta attttggatt
     2161 attcataacc taactccatt tcttccattc aattatttat attttctcta ggcacagatt
     2221 gtccgatctc ttaaagaaag acaatttcat atagggctgt gacaaaagcc gtgtagcagt
     2281 ctctttttcc gagtaaacgg cgttctagag acagcctatc agcaataagc ccacccaagc
     2341 caaaaatacg aaaagctgtt tttgcttggc tgctcttatt acttaatgca ggatagcttt
     2401 ttcacaacct ttgtcaacta ctacaactga atcagtatag catggattca gcgaatgaaa
     2461 aagccaccaa accataagag tcaaatattt ttttgtcagc tgactaaaag aaaagacgtg
     2521 ttgcttgttt attcaagcaa cacgtctttc ataaccaagt ttccaattat cttcttggct
     2581 cgtcatcttc gaattctttt ctgaagtatc ttcgttcttc ttcaggttcc gcttctgttt
     2641 cttccggatc atgaaaaatg acacggattt taaggattcg agaaccttcc atttcttcag
     2701 aaaccagcgt aatattatcg acatcaaacg ataacttttc gccttcatcc ggaatcatgc
     2761 ctaatgctgt aatcaaataa ccagccatcg tatctacatc actcatatgc agatcactgt
     2821 caaaagcttc attgaactca tcaatcagca tacgtccttg gatgatatat tcgtgttcat
     2881 cgattttttc ataaagattc tcaacttcat ctgattcatc atcgatttct ccgacgattt
     2941 cttctaaaag gtcttctagt gttgctaacc caacgacgcc gccatattca tctaataaaa
     3001 tagccatctg attctgcgtc tttttcattt catataacag atcatcaata aagaccgttt
     3061 caggaacgaa taaaggctcc tgcatgattt tcttgatatc taaattatca aagccgaatt
     3121 tatgggcagc ttttagtaaa ttctttgtat gaaggattcc gactacttta tctttgtctt
     3181 cgttataaac agggattctt gaatagtttt cggataaaac ttcattcaca ttttcttcca
     3241 cagtgtcgtt gatatcaatc ataaatgcat ccgttcgtgg gaccatgact tctcgggcaa
     3301 ctttcgtatc aagagaaaaa accccttgga gcatttctaa ttcttcatta ttcaatacgc
     3361 cttcgttttc aagcatataa cgcatttcat ctctggtcat ttttgaatct tcatcatcaa
     3421 aagtcatcgg cgtgatacgc gccaataaac ttgtggaagc agaaagcagc cagacgaagg
     3481 gcttagctac tacaccaatc aagcgtatca gccctgaagt gaactttgcg acttcttctg
     3541 atttatttaa agcaatacgt ttagggtaca attctccaaa aacaatcgat acataggtca
     3601 aaatagccag cacaatgata tttgcaatat tttttgccgc tgtgccgcca cctaataccg
     3661 gagctaaccg ttccgataaa gtattagcta gtgaagcacc agacaaaatg ttgaccaaag
     3721 tgattcctac ttggattgtc gataggaaat tatttggatt ctgcaatatt tttaaaagct
     3781 tttgagattt cacatctcct tcatcagctt tttgctcgat tcgattctta ttgatcgaaa
     3841 caacagcaat ttcggaagct gctaaaaaag cattgatcaa tgtcaaaacg atcaataata
     3901 aaatctgcgc taacagcgac tgactctcag ggtcagcatt cataatcgca ttctctcctt
     3961 ataaaattaa tttttagttt acgatagaaa aaaacgtggc actatagcca cgttaaaatg
     4021 gtatcacaaa gcaattttct ttgcaataaa aacaagtgta ctaaaactac ctacgaggtc
     4081 gtagcaattt tcttcttgtt ttcattttgc ttgtcttctt tgacgatttt gattatatag
     4141 gaaatgatcg tcttgcagat tgcgtaaaaa ggaacaccta agaagatacc aagcaatccg
     4201 gcaatatttc cagctactag aagaacgata atgatcgtta atggatggat tttcagtgat
     4261 ttcccaatta cattaggata aatgatattc ccatccaact gttggacgat tagcacaacc
     4321 acacaacaaa gtaatgcttt taccggatca ttgaagactg ttactagaaa tgcaggtgct
     4381 agtcctagat aaggtcctaa atatggaatt aggtttgtca atcccgcaat gaccccaaat
     4441 aagaaagcat aatccacacc aattaccata tatcctaaaa atgtgaaggt accaacgaat
     4501 aaacattcga ttgcttgtcc gctaatgtaa ttagccaatg ttttattcag ttgccccaat
     4561 aactccacaa tttggtttct tcttttttct gggaaaaact gttggatgtt tggcactagt
     4621 ttttctccgt ctttcaacat atagaacaag atgaatggtg ctgtaatgat tactacggtt
     4681 gccgaagcga tcgttccaac gatcgaacct aaactattgg atactccact aaggaattgc
     4741 tgaatgattg tcccgtaaga aatgtctaat tgctcaaaat atttattgat atctacttcc
     4801 ttgaacaaag gcaattcggc tacctctttt aaccagcttt ctacctgttt gatgaaatca
     4861 ggcatattag atgccaatga ggaaatttgc tgtactagac ttgggatgac actaagtaaa
     4921 atccaaataa ttgctgcaac taaaaggagc agcacaattg cgacagccca tattcttttg
     4981 atttttgctt tcatcaacag cttgacaata ggattcaata aatagtaaag aaagcctgca
     5041 atcagtacag gtgcaaataa cgtggaaaaa aatgtcccga tcggttcgaa aataaagttt
     5101 atttctgatg aaatcatgat caatgcagct atgatcaata attcaagcga ccaaaacatt
     5161 agtttagact gtctaagttt ttcaaacaat agatttcctc ctttctttct tactagtata
     5221 tattatattc gttaaaaatt cttttattct ttttttattt ttgaaaactt gccatacttt
     5281 ttatttgttt tattttccag tatactatac gaaaggaagg tggctagacc atgaaagttg
     5341 tacaaacaaa agatacaatg agcaatattt acttagatgc tgtacggatc aggcatcaag
     5401 tttttgtagt cgaacagggt gtgcctttgt ctcgagaaat agacaaagat gaagcccatt
     5461 gtatccactt cgtcctgtac tcagataaaa aagaaccgca aggaactgta cgtcttttgc
     5521 ctttagaaaa cggaaaaatg aaattacagc ggatggcgat tctttctgaa tacagacacc
     5581 aagggttagg aaaaatattg attgaagaag cagaaaactt tgctaagaat caaggctata
     5641 atacgatttt attaggggca cagtccacag ctgaaacttt ttacgaaaaa ctaggatata
     5701 cagcttacgg cgatcctttt gaagatgctg gcatgcctca tatttatatg aaaaagactt
     5761 tataaaaatt tatttcgaaa aaagcaaaaa acagcaagct tttgagaaaa atctctcact
     5821 ggcttactgt tcttttttgt gtttacttac gcgacaattg cttgtaacgg ttataccaaa
     5881 tatcaatata gccttccgag aacgggccat ctccattatt gatccagtca accagaatct
     5941 tcacattttc ttttaggatg aagtcaatat cgttcgggta attcatcatt ttgctgtgca
     6001 tctcatattc atcgacatcc aatagtcgtt tctcgccgtc aggaaatacc ttgatatcca
     6061 agtcatagtc gatatatttc aaggcctcat cgtccagcaa aaaaggtgaa gctagattac
     6121 aataatagga aacccccttt tctctgatca ttgctattac attgaaccag tattttttat
     6181 gaaaataaac aatcgctggt tcgcgcgtta cccatcggcg tccatcagat tcagtgacta
     6241 atgtgtgatc attcacgccg atcatagagt gttcgcttgt ttttaatacc atggtatctc
     6301 gccacgttcg atgcaatttg ccgtcgtgct tgtaactttg gatcgtcaca aactctcctt
     6361 cttttggaac tcccatagca acccttcctt aagcagcatt ttgtgctttg tgtttgggtg
     6421 ttcattacac cgacatcata attattatat caataaaaat gagctttgcc tagaaaggat
     6481 tcctattgtt tcgatttttc tctaactgat ctaccagttt catttggacc ttaggaaaaa
     6541 cgaccgaatc aaatgcatca ggcttcaacc acttcgaagt tttgttctcg gtaaattctt
     6601 gttctgctcc ctcggttgcc cgtccgtaaa aaaggagaac atgccatttc agatgactaa
     6661 aaatatgagt aacttctcct aaatgtcgtg tctgccaaac aaagagttcc tgcttttcaa
     6721 atgggagatt ttgctcttct tctgctacta ggtcatcaaa cagatcgatt tctggctttg
     6781 tttcccattc ttttttcaat cgctcatact cctcttgtgt cacttccatc attggaaatg
     6841 tccacatatt cgccagcaat ttttggctat ctctttcttc aaaataatac gctccagaat
     6901 gattttgaag agctgcactg atataataaa cgtcttttgg ctttgctttt tttgttttta
     6961 caggaaaact cgtttggatt cctcgtttgt tagctaaaca aaaggcttgg atcggacaag
     7021 tctcacactt tggagaagtt ggtgtacaga tagctgaacc tagatccatc attgcttggt
     7081 tgaattcacc tggatgcttt tcatcaatga ttttccgcat cgcttcatca aagattttcc
     7141 tgctagaagc ttttgcgatg tctgcttcaa tgcaaaacaa tctgctgact actcgcatca
     7201 cgttgccgtc aactgcaggt tctggaagtc caaatgcgat acttgcaatc gcgcctgtcg
     7261 tatatggtcc tattcccttc aatgagctaa tttcttcagg cgtttgcggc atttccccat
     7321 caaattctga catgatctgt ttagcagcag cttggatgtt ccgggctctt gagtaataac
     7381 caaggccctc ccatgctttt aaaagctttt cttctggagc atttgctagc tcttcaattg
     7441 taggaaacca ttccatgaaa cgataaaaat aatcgatcac tgtgtctact ctggtttgct
     7501 gaagcatgat ctcagagatc cagatacgat aaggatcacg attatagcgc caaggcaggt
     7561 ttcttttttc ttgttcatac cattgaatga actgatcttg aaattctttt gtttctttat
     7621 ctgtccattt ttcccattct ttattcactt tcctcttcct tttccgtaat gaatcgctgg
     7681 atcatatcca aatcaaatcc tttttgatat aagctttgct ttacttttag atttcttttt
     7741 ttaggttcaa accttaaatt tcttcgccat agtttatctc cttgtttgac taaataatca
     7801 tattctattt cttgatctgc ttcttgcgga agatcagcta tcgcttgttg gatcacttct
     7861 tgagaaaagc ctttcgtcat caatgtttgt tggatttttc tcaataattc tttttggctt
     7921 tttccgtgat tttttccaaa cgctttttcg gctgtctgat gtgcattttg aacttggtct
     7981 tcaaatgtat attggctgag cgcttgttca atgatttcag gtttcaaacc tttttgctgc
     8041 atctgctgcg ctaatttctt tggtcctttg tcacttaagc gcatattcgt gcgcacataa
     8101 ctttccccat agaccaaatc atcgacaagg tccaattctt ttaagcgggc tacgatttta
     8161 tttcgatctt cttgaaggat ttccttttct tttaagtatg ttcggatttc tttttccgat
     8221 cgcaactgat aacttaagta attcattgct aggtgaaaac cgaaatcagc tccgctactc
     8281 ttcttgatct cttggatagt ttcttcgtct aattcccgtc cttttaataa tcgatagcgt
     8341 actaatacat cttcagatac ttgtaaagct tctccttcag ataaatctac gcgatagaaa
     8401 gggcctttgc ctttactgat tctcgtaacg gttaacataa acatcctcct cgaacatata
     8461 ttctctatca taccacagaa aatccccgta atcaggtatt atatgttaga ataaaccaca
     8521 gaaaaaagaa aaagggagat acttatggca gaaacaattg tcaaaactgg acaaacggtt
     8581 cctttgaaaa tcaagcgttt aggcataaat ggtgaaggaa tcggttattt caaacggttg
     8641 atcatatttg ttccatatgc actaccaaaa gaagaagtac tagtgaaaat cacgaaagca
     8701 actcctcgct atgcagaagg agaattaata aaagtcaaaa aaacaagcaa agatcgcgta
     8761 gtggcaccat gtcctgttta ctatgaatgc ggtggctgtc aattacaaca cttagcttat
     8821 caagctcaat tggattttaa aaaggatctg cttttgcaag cattggaaaa attcaaacca
     8881 gcaggctttc gatcttatca acttttgccg acgatcggga tggatgagcc ttggcattat
     8941 agaaacaaag cacaatttca gttacgaaaa aataagcaga ctcaaaaaat tgaagctggt
     9001 ttatacgaag caaactctca tcagttagtc cctataacag actgcttggt tcaagaacct
     9061 cgaacacaaa aagtaatgaa tactgttgtt caactattga ataaatttga tgcacccatc
     9121 tatgatgaac ggacaaacag tggaatcttc cgcacaatca tggttagaat cggtgttaaa
     9181 actggggagt tacaagttgt ttttattact cgcagtaaaa aattcccgca aaaaaacgca
     9241 atgatccgtg agatcaatga acgactgccc gaagttgttt caatcatgca aaatgtccag
     9301 gcacaaaaaa cgtctctcgt gatgggggat gaaacgatcc acttatgggg aaaagaagcc
     9361 attgaagaac aaatcaatga agtgactttc gatctttctc ctcgtgcctt ctttcaactc
     9421 aatcctcagc agaccgaatt gctttatggc gaaggaatca aagcattaga tgtccagcct
     9481 aatgaaacga tcgtggatgc ttattgcgga gttgggacga tcggcttaag tgtagcaaaa
     9541 caagccaaag aagtacgggg aatggatacc gttcctcaag caatcgctga tgcaaaaaaa
     9601 aatgcggaaa gattaggata tcacaacact cgatatgaag tcgggacagc agagcaagtt
     9661 ctgcctaaat ggttgaatga aggttttcag cctgatggta tcatcgtaga tccaccacgt
     9721 acgggtttgg ataacgcatt gatccaatca ttattgaaat accctccaaa aaaacttgtt
     9781 tatatctcct gtaatgtttc tacacttgcc agagatctgg ttaatttaag taaaaagttc
     9841 aagattgaat atttacagtc cgtcgacatg tttcctcaga ctgcacgttg tgaagttgtc
     9901 gtgaagatga cgagactcgc tgactaaatg aaagaggttg tgaaaaagtc tgccctgtat
     9961 caagtaataa gaacagccaa acaaaaatag ctcttcatgt tttatgaata ctgtacttgt
    10021 gaccgaagag ttaacaggag gacactattt atttaaaaaa agggttgtga catagttttt
    10081 gtcacagccc tttttgatta ttattggata ttcacgtcta tcacttgata aaatgcattt
    10141 gtcgtatcgc tgatattcca tactgctaaa atcacattat atccttttcg atcctgcgga
    10201 agtgtgatcg tttggttgac tgtttttggt ggaacagttg ctttgtcgtc tatttttgtg
    10261 atcaattcaa aatcactaaa tttcaaaggt tgattaggat tccaagttgg tttggtaatg
    10321 tagtaatccc aagtacttgt ccgatgttgt gctgttaaat tccaagtaat atttaatgtt
    10381 cctggattaa ccatgttttt atgccatctg ttagatgtct gttcatctaa aggctcaaag
    10441 ccgctgactc ctgcactagc aatttttcca tcaatgaatg tatttttggt agcttcgata
    10501 ctttgcggtt cccattgtgc tcttccgaca ttttgattta agtttcctgc tgctgatgtt
    10561 cctaggtatg cacggcttcc tggagaagta atataaccat gcgcaaaacc tggaatgcta
    10621 aaaccgaaca accctaaact aactaaccca ataaacaata acgtactaat ctttttcatg
    10681 tagaaacctc cttcatttga taaccacatt gtaaacgctt tattgtatta acacaaacat
    10741 tatgctttta taattttatg ttgaagtaaa aaaggtgaaa aaagtaagat gcagattata
    10801 acagaagtag acgagaaaag tacaaataga aaacatgaga atcaaatcat atgatctttt
    10861 tcaaaaacat cattcatata aacaaatgtt caacttttta ataacaacaa ttatcaattt
    10921 ttttcttact tcacaaacta aatacaggtc ttgctgtgag ccttcatggt aaactaatat
    10981 tatagaaagg agttagtaaa atgaagatga taaaagatat tcgtttttgg gtttgtgtta
    11041 taggaattgt catattaggc tttttatcag ggttgcttag cggaaatccc ggaaaatatt
    11101 actattcttt gcaacttcca ccatttgcac cacctagctg gatatttggg ccgatgtgga
    11161 ccttactgta tatattgatg ggtatttctc tttatttgtt acttaatcac aacaacaaaa
    11221 aacagcgaaa caatcttgtc ggtctttttg tgattcagtt catttttaat tttatatggt
    11281 ctgctctatt ttttaattta agaaatattt tcattgcagc aatcgatatt acacttttag
    11341 ttattttctt gtcagtgctt atgtatcagt tgtggctgca tcatcgcctg gcaatgtggc
    11401 tcatgattcc ttattattta tgggtactat tcgcaactct attgaattat tcaatttatt
    11461 ttttaaacta agcgctaaaa agagacgtcc tgtaatttaa cacatagaaa aagtgccacc
    11521 taagttagat tttttgtcta atttaggtgg cactctttat taaccaaact atttttttct
    11581 agtacccact tgttgttcca tactcattat tctggtcata accataacca ttttgttcct
    11641 gtccagcgta aggatcataa ctttgagtat cttgacttgg tgtatagtac tgacttgtat
    11701 cttctgtttg ctgataatag gtattcggat cttggtactg atcatatgtg ttttctgaat
    11761 aggaagaata atcggtgtac ccttcactat tcgatccatc accgtttgtc gaatcgccat
    11821 cttccgaatc atcaaagaac atcattgaag cattctcatt tttcaaatta ggtaatgtat
    11881 cgcttgtttt tatatttaat tgtttcttca gttcattttg gatcgaaaga agatcatttt
    11941 ttcctaaaat ttgatagtag atatcattga tcatttgtcc ttcgccttgc aactggtctt
    12001 gtttgattgt ttcaaacgcc ggggtgtagt tcgtcgctac atcaagcatg tcatcccaat
    12061 ctaagtccgt cgtcacattg ttctcaactg cttttaaaat ttttctataa ttgcttacgc
    12121 catctaaact taaaacttta ttgacgattt tcgtcactac ttcccgctga cgacgttgtc
    12181 gaccgatatc gccttctgga tcttcgtgtc gcattcgaga ataggctaaa cctttttctc
    12241 cattcaatgt ttgttcacct actggtacat ggacaccatc caaagtaaag tcaattttat
    12301 tattcacttt gatgccaccg accgcattga tcaaatcttc catgccatcc aaattgatcg
    12361 ttacataatg atcaatagga atatcgagca gttgctcaat tgaatccatg gccatttcga
    12421 caccaccaaa tgcgtaggca tgatttaatt tatctaccgt tccataacct acgatattcg
    12481 tataaatatc tcggtccaaa ctgatgatcg ttgatttttt ttgtttcgga ttaactgtta
    12541 ctaccatcat cgtatcagaa cgtccttgtt ccgttcttcc taatccaccg gtatctaaac
    12601 ctaatagcaa aaccgaaaaa ggttctttgt catcaatgct tactctctca gaacgtttgg
    12661 aggatattcg attagagttt ttagaaattt tgtttactgt ttggctcgct tctcccataa
    12721 atctgattcc gtatgctgat aatccggcaa tcagcaaaac gaatacccct aacaaggtta
    12781 atagaacttt ttgccatcgc ttcataatac acctctgttt cacattttct aaaatattgt
    12841 ctcatataac tgttcaatca gcaaattcac tttttaatca taagaaaact ttaaaaaaca
    12901 tttttgtgta acgattatta ttttttaaaa tttctcaaaa ttcttaacta tcataccata
    12961 ccgtgaaaaa gatcgaaaga ccgaatacac gcagttcgca atcgttttat ctttttttat
    13021 gtattttttc cagaaaaagc gaacaacaag gtattttcca aataatcgtg cagtaaaatg
    13081 atcgcacata cctttcccat ctttttaaca gtctttgcct accgttcaga ttatcattcg
    13141 atttcacgac aatttcttcc tatgactggt ccttttgtta aacccatctt ctattcgttt
    13201 tatttccagc tcactgcatg gctcttaaaa tgactcgaac tagtgattgc attaccatca
    13261 tcttttaatg gatagatatg ctcctcttca aagtaaaacg attttctttt aaagtaatag
    13321 aattgacatg gtaatgagta tgtatactag tggtttcaca aatggtcttt tattcaacat
    13381 ccaaaaactg aaatttcttt aaaatccatt actaacgaag aaaatgttcc ttgcttgata
    13441 attagttagg acatgtcaag tgaacaaatg catttactta gccaaaaaat ctaaaaactt
    13501 cataaataga caaaaagaca cgactctcta agaagtcgtg tctttattca acaaattatt
    13561 ataatttagt cacttcagca gcttgaggtc cgcgtgcacc ttcaacaatc gtgaattcta
    13621 cttcttgacc ttcttctagg ctcttgaagc cgtctccttg aattgctgag aaatgaacaa
    13681 atacgtcatc tccgccgtct actgtaataa acccaaatcc tttttcattg ttgaaccatt
    13741 taaccgttcc gtgttccata aatgtatatc ctccaaaaaa gttcttgcaa gtaaccttgc
    13801 aagaacatta tacccaattt agaaaacgct agcaagcttt cgctctttta ttttccaaat
    13861 tatttttaag actgatatga cggaggaaat ggttattttg ctgcttcgaa acgtttgttt
    13921 acttcatccc aattaataac attccagaat gctgcaatat aatctggtcg gacattttta
    13981 tatttcaaat aataagcatg ttcccaaacg tccaaaccta gaattggtgt ctttccttcc
    14041 atcaatggag aatcttgatt tgcagtagag gtaatagcta attttccatt ttccattaca
    14101 agccaagccc atccagaacc aaatcgtcca gcggctgctt ttttgaattc ttctttaaaa
    14161 gaagaaaaat caccaaaagc ttcattaatc gcttctttta tttctcctgt aggttcgcca
    14221 ccagcatttg gtgccatgat ttcccagaaa aatgaatggt tagcatgtcc gccaccattg
    14281 ttacgtacag ctgtcttgat atctgttgga atagcgtcca tatcagataa taattcttct
    14341 attgtttttt cgcctaattc tgggtatttc tcaattgctg aatttaaatt cgtcacataa
    14401 gtattgtgat gcttatcatg atgcagatgc atcgtttctt cgtcaatata aggttccaat
    14461 gcgtcatatg cataaggtag atctggtaat gtgtaagtca taaaaaaatt cctcctcaat
    14521 gttttcttaa taatgtctac attacaaa
//
LOCUS       DAZKLM010000068        14535 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3764
            SAMEA115706913-rid22582563.denovo.070, whole genome shotgun
            sequence.
ACCESSION   DAZKLM010000068 DAZKLM010000000
VERSION     DAZKLM010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706913
            Sequence Read Archive: ERR13196647
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14535)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14535)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287823.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287823.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,004
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,849
            CDSs (with protein)               :: 2,849
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 41 of 96
            Pseudo Genes (incomplete)         :: 52 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 15 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14535
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706913-rid22582563.denovo.070"
                     /isolate="VRE3764"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(17..664)
                     /locus_tag="ACG842_001955"
     CDS             complement(17..664)
                     /locus_tag="ACG842_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0248487.1"
                     /translation="MEAKITLEPFERILSGYRKVEELAVNVTDCSKLAQKYARFGVEG
                     YRLGNYVGTGYLNRYLECMVDRAPMLIYRQNYLIPLLFRRSDSAFRLFEEEYRMEAFF
                     LLLEWSLKHRPEKILIERNEKIDTKKNKVIDSAYLAFRVSEILDCGGYPISNFQSIDQ
                     FIEWNRIYRLIDNGGIGRHSKVFDPEYPENMEELKMIISLVKLKYPETDLDLYIE"
     gene            complement(843..1562)
                     /locus_tag="ACG842_001956"
     CDS             complement(843..1562)
                     /locus_tag="ACG842_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFQ0248488.1"
                     /translation="MGRKWANIVAKKTAKDANNSRIYAKFGIEIYAAAKSGDPDPHAN
                     QKLRFVIDRAKTYNVPKHIIDRAIEKAKGAGDETYSELRYEGFGPSGSMVIVDTLTNN
                     VNRTAADVRAAFGKNGGNMGVSGAVSYMFDNTAIFAFAGEDADEILEYLMEKDIDVRD
                     VMEEEGQIIVYGEVADFHSIQEALKEKGITDFSIAEIQMIPQNEVTLSPEDQETFEKM
                     IDALEDLEDVQHVFHNVALED"
     gene            complement(1693..2844)
                     /locus_tag="ACG842_001957"
     CDS             complement(1693..2844)
                     /locus_tag="ACG842_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HFQ0248489.1"
                     /translation="MKSCGIIVEYNPFHNGHRYHVEMARKITGAEVVIAVMSGNFLQR
                     GEPAIIDKWHRANEALQNGVDLVVELPVEWAVQSADYFAKGAVKILQTLDCDSLCFGT
                     EEETSFDYSSFGRFVQENQDRIEEAFQELSDQTYSYPQKMTEVFRSLYPDITLDFSSP
                     NHILGLSYAKENAKYVHPMELHPIRRKSAGYHEKIITDQRIASATAIRHALEEGKRIA
                     GLVPPQTEEDLSTCSLQTWESYWPYLRYRLLSSTLLELTDIYQMTEGIESRMKTAAAT
                     ADSFEDFVSKVKTKRYTWTRIQRLSCYVLLNIKKQEIPVQQNKNYLRLLGFTDAGRRF
                     LKEKKEISIYSRIGKKEAQEAELLVRSDQIYQLGGNIPEQNFGQIPIRV"
     gene            complement(3061..3801)
                     /locus_tag="ACG842_001958"
     CDS             complement(3061..3801)
                     /locus_tag="ACG842_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFQ0248490.1"
                     /translation="MAYETFAFIYDEVMDETLYQQWLAFSKRHLQEDTKNILELACGT
                     GALAVAFAKDGYKVTGLDLSEEMLMIASQRAYEEDASIQFVEGNMLDLSEIGQYQAIT
                     CFSDSLCYMKNRQEVQQVFDEVYQALEENGRFLFDVHSTYQVDEVFPEYSYHYQGDNF
                     AFLWDSYPGSEKHSIEHFLTFFVEEQEGSGRFIREDELHQERTYPLESYLRMLENSGF
                     SKVEAFSDFTDEAASEKSKRWFFVAYKD"
     gene            complement(3805..4128)
                     /gene="rsfS"
                     /locus_tag="ACG842_001959"
     CDS             complement(3805..4128)
                     /gene="rsfS"
                     /locus_tag="ACG842_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HFQ0248491.1"
                     /translation="MEAADSKRAEDIIALDVRNVSLLADYFMICSANSERQINAITEE
                     IIDKEEENKYEVKRIEGKEGGKWVLIDLGDVIVHVFHAPERNFYNLEKLWSDAPLVDL
                     SEWLD"
     gene            complement(4185..4778)
                     /gene="yqeK"
                     /locus_tag="ACG842_001960"
     CDS             complement(4185..4778)
                     /gene="yqeK"
                     /locus_tag="ACG842_001960"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HFQ0248492.1"
                     /translation="MNMDYSNEYTAYSRENLMQKIQMRMSEQRFKHVLGVEETAVALA
                     KKYGASPEKASIAALTHDYAKERPDEEFKMVIVRDGFDPELLNYNNAIWHGLVGASFV
                     ERELGITDAEILHAIRVHTTGAAKMSLLDKIIYVADYIEPGRDFPGVQDARAIAWADL
                     DEAVAFETKHTLAHLLAQEQQIYPKTIETYNYWVAKK"
     gene            complement(4762..5412)
                     /locus_tag="ACG842_001961"
     CDS             complement(4762..5412)
                     /locus_tag="ACG842_001961"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HFQ0248493.1"
                     /translation="MMNTQAKTFVRSQLFPEEMPQFLEKKKQVGILGGTFNPVHLAHL
                     VMAEQAGRNLGLDRVFLMPSYQPPHVDEKQTIDAKHRLNMLELAVEDNPFLQIETIEL
                     ARGGKSYTYDTMKELTQNNPDTDYYFIIGGDMVEYLPKWYKIDELTSMVNFVGIRRPG
                     YTTDTPYPVIWVDVPEIDISSTKIRQKIKEGCSIRYLVPDKVIDYIQNEGLYEYGL"
     gene            complement(5422..5733)
                     /gene="yhbY"
                     /locus_tag="ACG842_001962"
     CDS             complement(5422..5733)
                     /gene="yhbY"
                     /locus_tag="ACG842_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HFQ0248494.1"
                     /translation="MSLRGKQKRFLRSKAHHLQPIFQIGKGGINSAMIVQIEEALEKR
                     ELIKVSLLQNTDEIAEEAAQVLEKEIDCEIVQIIGRVIVLYKPSTKEKYQRLSKEVKA
                     I"
     gene            complement(5749..6858)
                     /gene="yqeH"
                     /locus_tag="ACG842_001963"
     CDS             complement(5749..6858)
                     /gene="yqeH"
                     /locus_tag="ACG842_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HFQ0248495.1"
                     /translation="MTEELQCIGCGAVIQTNKPGELGYTPQTALEKGLASGEVYCQRC
                     FRLRHYNETQDVSLTDDDFLRLLNELGQKDALIVNVVDIFDFNGSLIPGLHRFVGDNP
                     VLLVGNKVDILPKSLKKTKLTQWMKERAYEAGIRPVDVLLTSAKKSHEMTELLEKIEE
                     YRDGRDVYIVGVTNVGKSTLINQIIKNTAGVQDVITTSQFPGTTLDKIEIPLDDGHFL
                     IDTPGIIHRHQMAHYLGKKDLRIIAPLKEIKPKVYQLNEGQTLFLGGLARFDFISGNR
                     ASFIAYVSNDVNIHRTKLEKADEFYQKHVGGLLQPPRPDEVEAFPELVRFEFSVKEKT
                     DIVFAGLGWITVTAPVVIAGWAPKGVDVLKRKALI"
     gene            complement(6855..7382)
                     /locus_tag="ACG842_001964"
     CDS             complement(6855..7382)
                     /locus_tag="ACG842_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008962 - phosphatidylglycerophosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HFQ0248496.1"
                     /translation="MFSNYKPTWMVEAIYQITPAQLKNLGIKAVLTDLDNTLIAWNNP
                     DGTEELLTWILEMKNAGIPVVVVSNNNSKRVARAIEKFDLLYVARAMKPLARGINIAK
                     KQLDLADDEIVMVGDQIMTDIRGANRAGIRSILVKPIIATDSWKTQFNRFWEKRIMAY
                     LLAKHPDMGWRGGIE"
     gene            complement(7562..8347)
                     /locus_tag="ACG842_001965"
     CDS             complement(7562..8347)
                     /locus_tag="ACG842_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HFQ0248497.1"
                     /translation="MDKTAHDVVQLARAKERWTSLDFFEEIFDSFEECHGDRLFADDE
                     AIVGGIARLDGKPVTVIGIQKGRDLQENIRRNFGSPHPEGYRKALRLMKQAEKFNRPV
                     ITLINTAGAYCGVGAEERGEGEAIARNLLEMSDLKVPIIAIIIGEGGSGGALALGLAD
                     EVWMLEETIYAVLSPEGFASILWKDGSRAAEAAELMKITAPELRELGIVDRVIPETFN
                     GEQLPRYKVAHIMKKALIERLTELVRLDQHKLLEKRYKRFRQY"
     gene            complement(8340..9209)
                     /gene="accD"
                     /locus_tag="ACG842_001966"
     CDS             complement(8340..9209)
                     /gene="accD"
                     /locus_tag="ACG842_001966"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HFQ0248498.1"
                     /translation="MALFKKKKYIRINPNRESKPANSPSVPDNMWAKCPNCKHILYTK
                     DIGEEKVCPHCGYAFRIGAWQRLALIIDEKSFEEWDTDLVTKDPLAFPEYTEKIKKMQ
                     DKTGLHEAVLTGKATIQGIPFIIGVMDPNFIMGSMGTIVGEKITRLFERATKESLPVV
                     LFTASGGARMQEGIFSLMQMAKISAAVKRHSNEGLFYLTVLTDPTTGGVTASFAMEGD
                     IILAEPQSLIGFAGRRVIEQTIKQELPEDFQKAEFLLSHGFVDQIVPRMELKQKIHTL
                     LELHTQKGWIDRG"
     gene            complement(9210..10589)
                     /locus_tag="ACG842_001967"
     CDS             complement(9210..10589)
                     /locus_tag="ACG842_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HFQ0248499.1"
                     /translation="MFSKVLIANRGEIAVRIIRACRELGIQTVAIYSEADAEALHTQL
                     ADEAICIGPAKAVDSYLNVQEVLSAAIVTKAEAIHPGFGFLSENSRFASMCEECNITF
                     IGPKSATIDAMGNKINARQLMQEANVPVIPGSTGVLSTVEEALEIADRIGYPVMLKAA
                     AGGGGKGIRKVQSKEELPQHFSSAQQEAAAAFGNDDMYLEKIIYPARHIEVQILGDRF
                     GHVIHLGERDCSLQRNNQKVLEESPSVVISQTKREALGDAAVRAAKAVNYENAGTIEF
                     LMDEEGDFYFMEMNTRIQVEHPVTEMVTGIDLVKKQVEIAAGEPLNVCQEEVVFQGHA
                     IECRINAENPAFHFAPSPGKIQNLLLPAGGMGLRVDSAVYPGYTIPPYYDSMIAKIIV
                     HGNTRFEALMKMQRALSEFITEGIITNAEFQMDLISHPAVIAGDYSTAFLQEEFLPNW
                     TPETEIGGA"
     gene            complement(10597..11016)
                     /gene="fabZ"
                     /locus_tag="ACG842_001968"
     CDS             complement(10597..11016)
                     /gene="fabZ"
                     /locus_tag="ACG842_001968"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HFQ0248500.1"
                     /translation="MNIQEIKEIIPHRYPMLLIDRVEEMVEGERIVAKKNVTINEPFF
                     QGHFPEEPVMPGVLIVEAMAQAGAVALLSLEQFKGKTAYFGGLDKAKFRKKVTPGDTL
                     YLEVEILKVKASAGIGKGIAKVDGKKVAEAELTFMIG"
     gene            complement(11041..11517)
                     /gene="accB"
                     /locus_tag="ACG842_001969"
     CDS             complement(11041..11517)
                     /gene="accB"
                     /locus_tag="ACG842_001969"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HFQ0248501.1"
                     /translation="MDINEIRELVSQFDQSSLTEFDLREGQFELYMNKNKTSRGMNMV
                     QTNETLPVSEPVNVIPEQATTAPEIMKEKESAVFDGIEVVSPIVGIVYLQPAPDKPAF
                     KKVGDQVAKGEVICIIEAMKLMNEITSEADGVITEILVENESVVEYGQPLFRIQQK"
     gene            complement(11520..12755)
                     /gene="fabF"
                     /locus_tag="ACG842_001970"
     CDS             complement(11520..12755)
                     /gene="fabF"
                     /locus_tag="ACG842_001970"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HFQ0248502.1"
                     /translation="MNRVVITGYGVTSPIGNDADSFLNSLKEGTNGIAPITKFDASET
                     GITLAGEVKDFPYDKYFIKKDSKRMDMFSIYGIYAALEAMEMSGLDKEKMNQDRFGVI
                     IGSGIGGLPTIENQVIRLHEKGAKRVSPMFVPMAIANMAAGNIALRVGAKGICTAIVT
                     ACASGTNSIGEAFRNIKHGYSDVILAGGTEATICEMGIAGFAALTALSESTDPNRGSI
                     PFDENRNGFVMGEGAGVLVLESLEHAQARGAKIYGEIVGYGANCDAYHMTAPTPDGSG
                     ASKAMKLAMEEAGVQPEQVGYINAHGTSTPANDQAEAKAIQIALGENFRDTYVSSTKS
                     MTGHLLGAAGGIEALATLLALEHQFIPPTINVEKQDPEIDLTVVKNESKPASFTYAMS
                     NSLGFGGHNAVLCMKRWEE"
     gene            complement(12774..13511)
                     /gene="fabG"
                     /locus_tag="ACG842_001971"
     CDS             complement(12774..13511)
                     /gene="fabG"
                     /locus_tag="ACG842_001971"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HFQ0248503.1"
                     /translation="MDVKGKNVFITGSTRGIGKAMALAFAKAGANIILNGRGEIPKEK
                     IEEIEAFGVKCVGVSGDISDYEKAGQMIKEAEEKLGSIHVLVNNAGITNDKLVMRMDA
                     EDFKKCLDINLIGTFNMTQHVLKKMMKQREGAIINLSSVSGLIGNIGQANYAASKAGV
                     VGLTKSVAREAATRGITCNAIAPGFITTDMTEVLADKVKEQAEKQIPMQRFGQVEDIA
                     QTAVFLAQNPYITGQVINVDGGLVMHG"
     gene            complement(13523..14440)
                     /gene="fabD"
                     /locus_tag="ACG842_001972"
     CDS             complement(13523..14440)
                     /gene="fabD"
                     /locus_tag="ACG842_001972"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HFQ0248504.1"
                     /translation="MKTAFLFSGQGAQYQGMGKDLYEEAVVKQTFDEASEILGYDMAE
                     LCFTENERLDQTQYTQPAILTVSIAYYRLLKEHGIIPDAALGLSLGEYSALVASNALS
                     FTEAVALVAKRGAYMTEAAPAGSGKMVAVMNAPIETIEESCHEASKYGIVSPANYNTP
                     QQIVIGGEEKAVDEAVLLLKEKGFKRMIPLNVSGPFHTAILEPAAKKLAKDLSQIQFS
                     EPSFPIISNTTTEIMKKETIAGLLEQQVMQPVRFYESIHKLKTIGIEQVIEVGPGKVL
                     SGFMKKIDKTIPVLRVENKQTFDETIAIL"
BASE COUNT         3838 a         3329 c         2503 g         4865 t
ORIGIN      
        1 aatcatttaa ggtcatttat tctatatata aatccaaatc tgtttcagga tattttaatt
       61 tgacaagcga aataatcatt ttcagttctt ccatgttttc tggatattct gggtcaaata
      121 ccttgctatg acggccaatc ccgccattgt cgatcaatcg gtaaattcga ttccattcaa
      181 tgaattggtc aattgattgg aaattgctga ttggatagcc gccacaatcc aaaatctctg
      241 atacacgaaa tgctaggtat gcactatcga tcactttatt ttttttcgtg tctatcttct
      301 catttctttc gataagaatt ttttcaggac gatgtttcaa gctccactct aatagtagaa
      361 agaaagcctc catccgatac tcttcttcaa ataaccgaaa ggctgaatca gaacgacgaa
      421 aaagcagcgg aatcagataa ttttgtctat aaatcagcat tggtgcgcgg tccaccatac
      481 actctaaata acggttcaga taccctgtac cgacatagtt tcctaatcga tagccttcaa
      541 cgccgaatct tgcgtatttt tgcgctaatt ttgaacaatc agttacgttc acagctaact
      601 cttctacttt gcggtaaccg cttaagatac gttcaaaagg ttctaaagtg atttttgctt
      661 ccatattctt ccccgtccat cttccacata cttcatatca tttcacatga tacttatgct
      721 ctcatcatac cacaggttga attagaaaag aaaataaatc acacaaaaaa gcaagacaat
      781 ctgttagtga gattgtcttg cttttcgaaa aagactgaat acttttcgtt aaaactggtt
      841 gcttagtctt ctaatgccac attatggaac acatgctgta catcttctag atcttccagt
      901 gcatcaatca ttttttcaaa tgtttcttga tcttctggag atagtgttac ctcgttttga
      961 gggatcattt ggatctcagc gattgaaaaa tcagtgattc ctttttcttt taatgcttct
     1021 tgtatactat ggaaatccgc tacttcaccg taaacgatga tttgtccttc ttcttccatt
     1081 acatcgcgaa catcgatgtc tttttccatt aggtattcaa ggatctcgtc agcgtcttct
     1141 cctgcaaaag caaagattgc tgtattgtca aacatgtaag aaaccgctcc ggaaacaccc
     1201 atgttcccgc catttttccc aaatgctgcc cgtacgtctg ctgctgttcg attcacattg
     1261 ttcgtcaatg tatcaacaat caccattgag ccgcttggtc caaatccttc atagcgcaat
     1321 tcagagtatg tttcatctcc tgcacctttt gctttttcga ttgctcggtc aataatatgt
     1381 tttggtacat tataagtttt agcgcgatca atgacgaaac gtagtttttg gtttgcatgc
     1441 ggatcaggat cacctgattt tgctgccgcg tagatttcaa tcccaaattt tgcataaatc
     1501 cggctattat tagcatcttt agcagttttc tttgctacga tatttgccca tttacgtccc
     1561 atattttcac ttcgctttct gatttgatag tttcataacg caaaaacgtc ctgttccatt
     1621 atacttgtga atcacgattc tgtgaagctt tttttctttc ttccttcatt cttctcattt
     1681 ttagtcaatt atctatactc ggattgggat ttgcccgaaa ttctgttccg gaatattgcc
     1741 tcccaattga tagatttgat cacttctaac taacagttcc gcttcttgtg cctctttttt
     1801 tccgattcgg gaataaatag aaatctcttt tttttctttt aagaatcttc ttccagcatc
     1861 ggtaaatcca agtagtcgaa gataattttt attttgttgg acaggaatct cttgcttttt
     1921 gatattcagc aagacataac aagataatcg ttggatccgt gtccatgtat atctttttgt
     1981 ttttaccttt gacacaaaat cctcaaaact atcagcagtt gcagctgctg ttttcatgcg
     2041 cgattctatt ccttcggtca tctgatagat atctgtcagt tccagcaaag tagaagataa
     2101 caaccgataa cgcaaatacg gccaataaga ttcccaagtc tgcagcgaac aagttgaaag
     2161 atcttcttct gtctgaggcg gcacaagtcc agctattctt tttccttctt ctaacgcatg
     2221 gcgaatggct gttgcactgg ctatcctctg atctgtgatg attttttcat gatagcctgc
     2281 acttttacgt cggatcggat gtaattccat agggtggaca tacttggcat tttcctttgc
     2341 ataacttaat cccaagatat gatttggcga agaaaaatca agcgtaatat cgggatacag
     2401 actgcgaaaa acctcagtca ttttttgcgg ataactatac gtttgatcag acagttcttg
     2461 aaatgcctct tctatcctat cttgattctc ttggacaaag cgtccgaaac tgctgtaatc
     2521 aaacgaagtc tcttcttctg ttccaaaaca caaactgtca cagtccagtg tttgcaagat
     2581 ttttactgca cctttagcaa aatagtcagc tgactgaacc gcccattcga cgggaagctc
     2641 cactactaaa tccacaccat tttgtaaagc ttcattcgcg cgatgccatt tatcgatgat
     2701 agccggttcg cctctttgca aaaaattccc gctcattaca gcaatgacga cctctgctcc
     2761 tgttattttt cgtgccattt ccacatgata gcgatgtcca ttgtgaaaag gattgtactc
     2821 tacgataatc ccgcagcttt tcatgcgcat cctccttcat agttatcgat aaaaaagaaa
     2881 tagagacaag taaaaaaagg caaaaatcgg aaaaaatacc cgaactatat aaactattct
     2941 cgcaaacaca atacttctgc gacgaaaagc tgcactgcaa gagcaatgcc tatatcgttc
     3001 ggatatataa tttatgttga agatttattc ttttcttgtt aactcgagca ggcaaaaccg
     3061 ctagtctttg tatgctacaa agaaccagcg ctttgatttc tctgatgctg cttcgtctgt
     3121 aaaatcagaa aatgcttcga ctttcgaaaa gccgctgttt tccagcattc ttaaataact
     3181 ttccaatgga taggtacgct cttgatgtag ttcatcttca cgaataaatc gaccagaccc
     3241 ttcttgttct tcaacgaaaa aggtaagaaa atgttcgatg ctgtgtttct cgcttcctgg
     3301 ataactatcc cacaaaaaag caaaattatc cccttgataa tgatagctat attctggaaa
     3361 aacctcatct acttgatagg tggagtgtac gtcaaataaa aatcggccat tttcttctaa
     3421 cgcttgatag acttcatcaa acacttgctg tacctcttgt ctatttttca tatagcacag
     3481 cgaatcagaa aaacaggtaa ttgcctgata ttgtccgatt tctgataaat cgagcatatt
     3541 gccttccaca aattggatcg atgcgtcttc ttcatatgct cgctggctgg caatcatcaa
     3601 catctcttct gaaaggtcta accctgtcac tttatatcca tcttttgcaa aggcaaccgc
     3661 taatgctcca gtaccgcatg ccagttccaa aatatttttt gtgtcttctt gtaaatgacg
     3721 tttcgaaaaa gctaaccatt gctgataaag tgtttcgtcc atcacttcat cgtaaatgaa
     3781 agcaaatgtt tcataagcca tatgttaatc caaccattca ctaagatcga ctagcggagc
     3841 atctgaccat agtttttcca gattatagaa atttcgttct ggcgcatgga aaacatgtac
     3901 gatcacatct ccaagatcga tcaataccca ttttccgcct tcttttcctt caatacgttt
     3961 tacttcatat ttgttttctt cttctttatc aatgatttct tctgtaattg cgttgatttg
     4021 acgttcgcta tttgctgaac agatcatgaa ataatctgcc aacaaggaaa catttcgaac
     4081 gtctaaagcg ataatgtctt ctgcccgttt tgaatctgct gcttcaatcg caatttttaa
     4141 ttgttcttta ctatctatga tgatttcctc ctaatatgag cagcttattt ttttgctacc
     4201 cagtaattat atgtttcgat tgtttttgga taaatctgtt gttcttgcgc aagcaaatgg
     4261 gccaatgtat gttttgtttc aaatgctaca gcttcgtcta gatcagccca tgcaatggct
     4321 cgtgcatctt gtaccccagg aaaatctcga cctggttcaa tatagtctgc tacataaatg
     4381 attttgtcaa ggagactcat cttagcagcc ccagtcgtat gcacccggat tgcatgaaga
     4441 atctctgcat cggtgattcc taattcccgc tctacaaacg aagcgccaac aagaccatgc
     4501 cagattgcat tattataatt caataactcc ggatcaaaac cgtctcttac gattaccatt
     4561 ttgaactcct catctggtcg ttcttttgca taatcatgtg tcaatgcagc aatactagct
     4621 ttttcaggag aagcaccgta ttttttggca agagccacgg ctgtttcttc tactccaagc
     4681 acatgcttga agcgttgttc gctcattctc atctgtattt tttgcatcag gttttctcta
     4741 gaataagcag tatattcatt actatagtcc atattcatac aacccttcat tttgaatata
     4801 atcaattact ttatctggaa caaggtagcg gatagaacag ccttctttga tcttttgacg
     4861 tattttcgtg gaactgatgt cgatctcagg tacatctacc caaattacag gatatggagt
     4921 atccgttgtg tatcctggtc gacgtatacc cacaaagttg accatagaag tcagctcatc
     4981 gattttatac cattttggca aatattctac catatcccca ccgataataa agtaataatc
     5041 tgtatccgga ttgttttgcg tcaattcctt catagtatca taggtgtaac ttttcccacc
     5101 acgagctaat tcaatcgttt caatctgcaa aaaaggattg tcttccaccg ctaattccaa
     5161 catgttgagt cgatgttttg catcaatcgt ctgtttctca tcaacatgag gtggctgata
     5221 agaaggcatc aaaaatacgc ggtcaagacc taaattccgg ccagcttgtt cagccatcac
     5281 taaatgtgca aggtgaacgg gattgaatgt tccgcccaag atccctactt gctttttctt
     5341 ctctaagaat tgaggcattt cttcaggaaa taattgtgaa cgaacaaatg tcttagcttg
     5401 cgtattcatc atggtcactc cttatattgc ttttacttct tttgatagcc tttgatactt
     5461 ttcttttgtg gaaggtttgt ataatacaat aacacgtcca atgatttgga cgatttcgca
     5521 atctatttct ttttctaata cttgagctgc ttcttctgca atctcatctg tattttgaag
     5581 taacgatact ttgatcagtt ctcttttttc taaagcttct tcaatttgaa cgatcattgc
     5641 tgaattgatc ccaccttttc cgatttgaaa aatcggctga agatgatgcg ctttgctacg
     5701 taaaaaacgc ttttgttttc ctcttaaact cataattttc cttcttcctt aaatcaatgc
     5761 tttgcgtttc aatacatcta caccctttgg cgcccagccc gcaattacta ctggcgctgt
     5821 tacagtgatc cagcctagac cggcaaaaac gatgtctgtt ttttctttca cagaaaactc
     5881 aaaacgaaca agctccggaa atgcctcgac ttcatcagga cgaggaggct gtaataatcc
     5941 acctacatgt ttttgataaa attcgtctgc tttttccagt ttcgttcgat gaatatttac
     6001 atcatttgac acataagcaa taaatgaagc acgattgccg ctgataaaat caaatcttgc
     6061 aagtccgccc aagaaaagtg tttgcccttc attcagctga taaactttag gtttgatttc
     6121 ttttaacggt gcaatgatcc gcaaatcttt tttacctaag tagtgcgcca tttgatggcg
     6181 atggataatt cccggtgtat caatcaaaaa atgcccgtca tctaaaggaa tctcgatttt
     6241 atccaaggtc gttcctggaa actgcgaagt tgtgattaca tcttgtactc ccgctgtatt
     6301 tttgatgatt tgattgataa gcgtcgattt tccgacattt gttactccga cgatatagac
     6361 atcccgtcca tcgcggtatt cttcgatttt ttctaatagt tccgtcatct catgagattt
     6421 cttagcacta gttaataata catctactgg acgaattcct gcttcgtaag cacgttcctt
     6481 catccattgt gttaatttag tttttttcaa tgatttaggt aatatatcca ctttgttccc
     6541 aactaaaagt actggattgt caccgacaaa acggtgtagt cctgggatta gtgaaccatt
     6601 gaagtcaaaa atatccacca cattgacgat caatgcatct ttttgcccta attcattcag
     6661 taagcgcaaa aagtcgtcat ccgtcaatga gacatcttgg gtctcattat aatggcgtaa
     6721 tctgaaacaa cgctgacaat agacttcccc ggaagctaaa cctttttcca aagccgtttg
     6781 cggcgtgtat cctaattcac cgggtttgtt tgtttgtata acggcaccac agccgataca
     6841 ttgtaattct tctgtcattc gattccacct cgccagccca tatcagggtg ttttgctaat
     6901 agataggcca taattctttt ttcccaaaaa cgattaaatt gtgtcttcca actatctgtt
     6961 gcgataatgg gttttacaag gatgctacgt atccctgcac gattggctcc acgtatatca
     7021 gtcataatct gatcgcctac cattactatc tcatcatctg ctaaatccaa ttgtttcttt
     7081 gctatgttga tccctcttgc taaaggtttc attgctcgtg caacatacaa aagatcaaat
     7141 ttttcaatcg ctcgcgcgac acgttttgaa ttattgtttg aaacaacaac aacagggatt
     7201 cctgcatttt tcatttctaa aatccaagta agcaactctt ctgttccatc tggattattc
     7261 catgcaatca acgtattgtc taaatcggtc aatactgctt tgatgcctag attctttaat
     7321 tgggcaggtg taatctgata gatggcttct accatccatg ttggtttgta atttgaaaac
     7381 atgctgtctc ctcatctgca aaaaatgaag gagcacataa agtactcctc aaccccgtat
     7441 tatactgcat atcagcaaaa aaaactacct ctatactcgt tcgtctttca gaaaaagcat
     7501 ttctttacaa aatgaccaaa aataaagatt gatcacagaa aaatttagaa acaaaaaaca
     7561 actaatattg gcggaacctc ttgtatcgtt tttccagtaa tttatgctga tctaatcgaa
     7621 caagttccgt caatctttca atcaacgctt ttttcataat gtgtgcgact ttatatcgtg
     7681 gcaactgctc gccgttgaac gtttcaggaa taactcgatc aacgattcct aactcgcgta
     7741 gttctggcgc agtaattttc atcaactcag ctgcttctgc ggcgcgacta ccgtctttcc
     7801 acaaaatcga cgcaaatcct tctggagaaa gcacagcata aatcgtttct tccagcatcc
     7861 agacttcatc cgctaaacct agagcaaggg caccgccact tccaccttca ccaatgatga
     7921 tggcgatgat cggtaccttt aaatcagaca tttcgagcag atttctggca atagcctctc
     7981 cttcgccgcg ctcttctgct cctacaccgc aatatgcacc tgctgtattg atcaaggtaa
     8041 tcaccggtct attgaacttt tccgcttgtt tcatcagtcg taaagctttt cgatagcctt
     8101 caggatgggg agaaccaaaa tttctgcgga tattttcctg cagatcccgt cctttttgaa
     8161 taccgatcac tgttaccggt ttgccatcta atcgagcgat tccaccaaca atcgcttcgt
     8221 catctgcaaa taaacgatca ccatggcatt cctcgaaaga atcgaagatc tcttcaaaaa
     8281 aatccaagct tgtccatcgt tctttagctc ttgctaactg aacgacatca tgtgctgtct
     8341 tatccacgat ctatccatcc tttctgtgta tgcaattcaa gaagcgtatg gattttctgt
     8401 ttcaattcca tgcggggaac aatctgatcg acaaaaccat gagataataa gaattcagcc
     8461 ttttggaaat cttctggaag ttcctgcttg atcgtctgtt cgatgacgcg tcttccagca
     8521 aaaccgatca agctttgggg ttctgctaga ataatatcgc cttccattgc aaaactggca
     8581 gttactcctc cggtcgtcgg gtccgttaaa acagttagat aaaaaagacc ttcattgcta
     8641 tgccgtttga cagcagcaga gatttttgcc atctgcatta gagaaaagat tccttcttgc
     8701 atccgtgcgc cacctgaagc tgtgaacaag acgactggaa gactttcctt tgttgctcgt
     8761 tcgaataatc tggtgatctt ttctccgaca atcgttccca tacttcccat gataaaatta
     8821 ggatccatca ctccaataat gaagggaatc ccctgaatgg ttgctttacc agtgaggact
     8881 gcttcatgaa gtcctgtttt atcttgcatt tttttaattt tttccgtata ttcgggaaat
     8941 gctagcggat ctttggttac aagatccgta tcccattctt caaaactttt ttcatcaata
     9001 atcaaagcta gacgctgcca tgcaccgatt cgaaaagcgt atccgcaatg agggcatact
     9061 ttttcttcac cgatgtcttt cgtataaagg atatgtttgc aatttggaca ctttgcccac
     9121 atgttatcag gaacagatgg cgagttagca ggtttgctct ctctattagg attgatccga
     9181 atatatttct ttttcttaaa cagagccatc tatgcgcctc ctatctccgt ttctggtgtc
     9241 caattcggta aaaattcttc ttgtaaaaat gctgtgctgt aatcacctgc tatgactgca
     9301 ggatgactaa tcaaatccat ctgaaattct gcgttcgtga tgattccttc tgtgataaat
     9361 tcacttaagg ctcgctgcat cttcattaat gcttcaaaac gcgtatttcc atggacgatt
     9421 atttttgcaa tcattgaatc ataatatggc gggatcgtat atcccgggta aactgcactg
     9481 tctactcgca gtcccatccc acctgctgga agcagtaagt tttggatctt ccctggagaa
     9541 ggagcaaaat ggaaagcagg attttctgca ttaatccggc attcgattgc gtgtccttgg
     9601 aatactactt cttcttgaca cacattcaaa ggctcaccgg ctgctatctc cacctgtttt
     9661 tttactaaat cgattcctgt gaccatttct gttactggat gttccacttg gattcgagta
     9721 ttcatttcca tgaaataaaa atcgccttct tcatccatca gaaactcgat cgttccggca
     9781 ttttcataat tgactgcttt agctgctcga acagcagcgt ctcctagtgc ttctcttttt
     9841 gtttgtgaga tcactacaga aggagattct tccagtactt tttgattgtt gcgttgtaaa
     9901 gaacaatctc gctcgcctaa atgaatgaca tgaccaaatc gatcacctaa gatctgaact
     9961 tctatatggc gagcaggata aatgattttt tccaaataca tatcatcatt tccaaatgcc
    10021 gctgctgctt cttgctgagc tgaagaaaaa tgctgtggga gctcttcttt tgattgaact
    10081 ttccgaatgc ctttcccgcc accacctgca gctgctttca gcatcactgg ataaccaatt
    10141 ctgtctgcta tttccaatgc ttcttctact gtagataata caccagtact tcctggaatg
    10201 actgggacat ttgcttcttg catcaattga cgcgcattga tcttatttcc cattgcatcg
    10261 atcgttgcac ttttaggacc aataaatgtg atattgcact cttcacacat ggaggcaaat
    10321 cggctatttt cagataaaaa accaaatcct ggatgaattg cttcggcttt tgtcacgatt
    10381 gcagcgctca gtacttcctg aacattcaga tatgaatcaa cggcttttgc cgggccaatg
    10441 cagattgctt catctgctaa ctgtgtatga agtgcttcgg catctgcttc tgaatatatc
    10501 gctacggttt ggatccctag ttcgcggcat gcacgaatga tacgtacagc gatttcaccg
    10561 cggttagcta tcaatacttt agaaaacata tttcacctat ccaatcataa aggttaattc
    10621 cgcttcggct acttttttac cgtctacttt agcgattcct ttgcctattc cggctgaagc
    10681 tttcactttc aatatctcaa cttcaaggta taaggtatct cccggtgtaa ctttcttacg
    10741 gaattttgct ttgtccaatc cgccgaaata agccgtcttt cccttgaatt gttcaagaga
    10801 aagtaatgca actgctcctg cttgagccat tgcttcaaca atcaataccc ctggcataac
    10861 aggttcttct ggaaaatgtc cttgaaaaaa gggctcattg atcgttacat ttttcttagc
    10921 aacgatccgt tctccttcaa ccatttcttc cactcgatca atcaaaagca ttggataacg
    10981 atgtggaatg atttctttta tttcttgtat gttcattgtt ttccctcttt cctgctttgc
    11041 ttatttttgc tgaatcctaa agagtggctg tccatattcg acaactgact cgttttctac
    11101 aaggatttct gtgatcactc cgtcggcctc actagttatc tcattcatca gtttcatcgc
    11161 ttcaataatg cagataactt ctcctttggc aacttgatca cctacttttt tgaaagctgg
    11221 tttgtcgggc gctggttgca gataaacgat tcccacgatt ggcgaaacaa cctcgattcc
    11281 atcaaatact gccgattctt tttctttcat tatttctgga gcagttgttg cttgttcagg
    11341 tatgacattg actggctccg aaactggcaa cgtttcgttt gtttgaacca tattcatgcc
    11401 tcgacttgtt ttatttttat tcatatacaa ttcaaattga ccttcacgca aatcgaattc
    11461 cgttagcgag gattgatcaa attgactgac taattctctg atttcattga tatccaaaat
    11521 tattcctccc agcgtttcat gcacagaacc gcattatgtc cgccaaaccc taatgaatta
    11581 ctcattgcat aagtaaaact tgcaggtttg ctttcgtttt tgacaactgt caagtcaatc
    11641 tcaggatctt gtttttctac attgatcgta ggtgggataa attgatgttc taatgccagc
    11701 aaagttgcca aggcttcgat cccgccggct gctcccaata aatgtccagt catcgatttc
    11761 gtgctgctga cgtaagtatc tctgaaattt tcacctaatg cgatttggat tgcttttgct
    11821 tctgcctggt cattggctgg tgtacttgtt ccgtgagcat tgatatatcc gacttgttcc
    11881 ggctgtacac cagcttcttc catcgcaagc ttcattgctt tagatgcacc agaaccatct
    11941 ggagtaggag ctgtcatatg gtaagcgtca cagtttgcac cgtatccaac gatttctcca
    12001 tagattttgg caccgcgagc ttgtgcatgt tctaaagatt caagaactag aacgcctgct
    12061 ccttcaccca tcacaaaacc attccgattt tcgtcaaacg gaatcgaacc acgattagga
    12121 tcagttgatt ctgagagtgc tgttaaagct gcaaatccag caatccccat ctcacaaatc
    12181 gttgcttctg ttcctccagc aagaatgaca tcagaatagc catgtttgat gttacggaat
    12241 gcttccccaa ttgaattcgt tccgctagca caagctgtta cgatagctgt acaaatacct
    12301 tttgcaccta cacgcaaagc aatatttcca gctgccatgt tggcaatcgc cattggtacg
    12361 aacattggag aaactcgttt tgcacctttt tcatgtagac gaataacttg gttttcgatt
    12421 gttggaagac caccgatccc agatccgatg atgacgccaa accggtcttg attcattttt
    12481 tctttatcta aaccgctcat ctccattgct tctaaagcag cgtagattcc atagatcgaa
    12541 aacatatcca ttcgtttgct atctttttta ataaaatatt tatcgtaagg gaaatctttg
    12601 acttcacccg ctaaggtaat ccctgtttct gaagcatcaa atttagtgat cggtgcgatg
    12661 ccattcgtcc cctcttttaa gctattcaaa aagctgtctg cgtcatttcc aataggtgaa
    12721 gtgactccat agccagtaat taccactcga ttcatttgtt tgctctcctt ttcttatccg
    12781 tgcatgacca atccgccatc gacattgatg acttgtccgg tgatatatgg attttgtgct
    12841 aagaaaactg ctgtttgtgc aatatcttct acttgtccga aacgctgcat cggaatttgc
    12901 ttttccgctt gttccttgac tttgtctgct aaaacttccg tcatatcagt tgtgatgaat
    12961 cctggcgcaa tggcattaca tgtaatgcct ctagttgcag cttctcgtgc cacagatttt
    13021 gttaatccta ctacgccagc tttactagct gcataatttg cttgaccaat atttccaatc
    13081 aacccagata cactagagag attaatgatg gccccttctc gctgcttcat catttttttc
    13141 aatacgtgct gcgtcatatt gaaggttcca atcaaattga tatccagaca ttttttgaaa
    13201 tcttctgcat ccattcgcat cacaagtttg tcatttgtga ttccggcatt gtttactaac
    13261 acatggatcg atccaagctt ttcttctgct tctttgatca tttgtcccgc tttctcataa
    13321 tcagagatgt caccggaaac tcctacgcat tttacaccga atgcttcgat ttcttctatc
    13381 ttttcttttg gaatctctcc gcgaccattc aaaatgatat ttgctccagc ttttgcaaaa
    13441 gctaaggcca tcgctttgcc gattcctctt gtactacctg tgataaatac attttttcct
    13501 tttacatcca taacgttcct ccttatagta ttgctattgt ttcatcaaat gtctgcttat
    13561 tctcaacacg cagaactggt attgttttat cgattttttt cataaatccg cttaatactt
    13621 ttccggggcc gacttcgatg acttgttcga tgccgattgt ttttaactta tggatactct
    13681 catagaaacg aacaggttgc atgacttgct gttcaagtag tcccgcgatt gtttctttct
    13741 tcattatttc cgttgttgtg ttgcttatga taggaaaaga aggttcagag aattgaatct
    13801 gacttaggtc cttcgccagt ttttttgctg ccggttctaa aatagctgta tgaaaaggcc
    13861 cgctgacatt tagagggatc attcttttga accctttttc ttttaatagt aaaacagctt
    13921 cgtctactgc tttttcttcg ccaccaatca cgatctgctg aggtgtatta taatttgcag
    13981 gagatacgat gccgtacttg ctagcttcat gacagctttc ctcgatcgtt tcaatcggcg
    14041 cattcatcac tgcaaccatt ttcccgcttc cagctggggc agcttctgtc atataagcgc
    14101 ctctttttgc tactaacgca actgcttcag tgaaggatag cgcattacta gctactaatg
    14161 ccgaatattc tcctaaactc agtcctaaag ctgcatctgg gatgattcca tgctccttca
    14221 ataagcgata atacgcgatg ctgactgtta gaatagctgg ctgtgtatat tgcgtctgat
    14281 ccaaacgttc attttctgta aagcaaagtt cagccatatc gtagccgaga atctcacttg
    14341 cttcatcaaa tgtctgcttt acaacggctt cttcatataa atcttttccc ataccttgat
    14401 attgtgcacc ttggccacta aataagaatg ctgttttcat gtttttctcc aatatatcca
    14461 gaatatatgt ggctctataa tttatgggac tgatgaatca gaattgattc ttcagcccca
    14521 taaattatag agcca
//
LOCUS       DAZKLM010000069        14416 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3764
            SAMEA115706913-rid22582563.denovo.071, whole genome shotgun
            sequence.
ACCESSION   DAZKLM010000069 DAZKLM010000000
VERSION     DAZKLM010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706913
            Sequence Read Archive: ERR13196647
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14416)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14416)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287823.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287823.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,004
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,849
            CDSs (with protein)               :: 2,849
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 41 of 96
            Pseudo Genes (incomplete)         :: 52 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 15 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14416
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706913-rid22582563.denovo.071"
                     /isolate="VRE3764"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(6..335)
                     /locus_tag="ACG842_001973"
     CDS             complement(6..335)
                     /locus_tag="ACG842_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4828 domain-containing protein"
                     /protein_id="HFQ0248505.1"
                     /translation="MIYFLGSLITVLSSFIYLKKKKQAEKSEGLSKYQGTWFFSDSQQ
                     KQQLQITSDYTFLINGQSLGTSVVEIDEYRLVVRDQYGFYLIFSYPEQTLYDESADTS
                     YKLLSEK"
     gene            512..1240
                     /gene="sfsA"
                     /locus_tag="ACG842_001974"
     CDS             512..1240
                     /gene="sfsA"
                     /locus_tag="ACG842_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HFQ0248506.1"
                     /translation="MKYQQVEIAFFIDRPNRFIAHCMKKDGEIIIAHVKNTGRGKEVL
                     IPGAEVAIVFAPGPKRKTAYDLIAVKKKGDWFNIDSQLPNRLAIDGILDGTILLPGLT
                     GPFTVYKREVTYLHSKFDIYLETVVGQKAFVEVKGMTLENKAIGAFPDAPTLRGLKHI
                     GELTYAAQDGYAAYVLFIAQFEHLHLATIHEEMQPALADMVRHAQQSGVQILAYNCQV
                     SADEVTVKQAIPFDVDIPFEDPIQ"
     gene            1256..2269
                     /locus_tag="ACG842_001975"
     CDS             1256..2269
                     /locus_tag="ACG842_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFQ0248507.1"
                     /translation="MINLGIIGTNWITHQFVQAALSTKKYELTAVYSRHLEKAQEFGS
                     HYEGDIEFATDLKTFFNITHMDTIYIASPNSLHFSQAKQAILAGKNVIVEKPAFSTPK
                     EMDEIIHLANEHNVYFFEAARNIHESGFKKIADYLPLKDEIIGANFSYMKYSSRYDQV
                     LDGEEPNIFSTHFSGGALMDLGVYLVYAAVAWFGMPKEVHYFPRKISTGVDGLGWGIL
                     RYENFDVSIQPGKIGDSYLPSEIYFDSGTLVMNGVNAIEQAQFHDRAHKEIIDLAVTA
                     AENPMADEAEDFANVMAHPKDPAWGLLYEEWIELARNVNQVIYDLRKNGGIKFDADNE
                     EDF"
     gene            2269..3546
                     /locus_tag="ACG842_001976"
     CDS             2269..3546
                     /locus_tag="ACG842_001976"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFQ0248508.1"
                     /translation="MNEFVQVFPENWQKKYEDKGFISPSLIQRAVFQPLSNKQSIVAV
                     SPTGSGKTLAYLWPLLLNVEPGEASALVIFASSQELAIQVADVAREWGKDKELKVQSL
                     VGGANVKRQIEGLKEKPEILIGTPGRILELMKAKKIKAHQVKTMVFDEADQLFDAGNS
                     QIIDQILHQAPTEYQLAFFSATADRSLESIEKITGKTFETIDVTAEDDSRKGLRHYFL
                     RVPIRKKDDYLRRLTHIEDFHGLIFFNQLTELGVMEEKLLYRGVPVGSLASDQNKLLR
                     KAALEQFKKKMVRALLTTDVAARGLDITGLPYVVNAEVPLSEEAYLHRSGRTGRMGNE
                     GIVITLVQDNNLRDLKRLLRPSNITLEEIFLYGGKLQTEQPTKEDAAPAVNKKYAKTF
                     SGKEQNPEYNKEKIRKKVKNKSKKERNKGARRK"
     gene            3609..6311
                     /locus_tag="ACG842_001977"
     CDS             3609..6311
                     /locus_tag="ACG842_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFQ0248509.1"
                     /translation="MKERQEEILQLLTENQQGLTASDVAERLTIDRSNASRYLSELYK
                     AHHIVKTAGRPVVYSLPTEKSKSDEVHVDSSTQVTFETLVGENDSLKVSIQQAKAAIL
                     YPPRGLHTIIFGETGTGKSMFAECMYHFAIDSEMLSADAPFVSFNCADYDQNPQLLFG
                     HIFGIKKGAYTGAAQDSPGLIAKADGGILFLDEIHRLPPEGQEMLFTFIDKGIYRPLG
                     ESAQVHEASVQIIGATTESSESFLSTFNRRIPMAITLPNLASRSLDERYEIISLFIKQ
                     EANRLNQRIDIEKEAILAFMLYDAEGNIGQVKRDLKLVCAKSFLHYRTHHEKKLIIRK
                     EELSLQVQKGLLKIKEIPERLDRFMDSKSQYLTFEPGFADVVWSQDPERNMQVYNDIE
                     EKVTSLSETGVENIDLETLISKDVDAYFQTYVKELTKSSIPKDLLPDEIWRLTNQLYD
                     VAEKRLDRIYNEKARFAFALHLQSTLDRVREGHMIVHPDLNNVRKNFKREFQVAIDLS
                     TIIEEEEQVEIPFDEIGFISMFLSINLGESEPVKENKVDVLILMHGRQTASSMLEMAQ
                     ELLGTKVGKAFNMPLVMEVQTMYEEVLQYVKNYQEHLTNGLLLLTDMGSLNTFSDLIY
                     EETGIRTKAITMTSTMIVIEAIRMASVGRSLEDIYQNIQLSFESIVRDQFKAEKSETL
                     QRKKAVIVTCFTGEGVAAKLYQRIMPVINQNKVELIQMQFLERESFKKHIDALLEEYE
                     IKAIAGTVDVDYQNIPFFSAYDVFDDEKLNILKRIADDEIPTAKIVDSLAGSLSDVFS
                     IDELIRSLQKTVQQIQTDLSIIVEPGVDTGIVIHLAFMLDGLIKQESHRQFEKLSIFS
                     KIYRLEMDIVRANLMALERKYQVKIPEDDIAFLTQMFLENKIKK"
     gene            6463..6780
                     /locus_tag="ACG842_001978"
     CDS             6463..6780
                     /locus_tag="ACG842_001978"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFQ0248510.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEEKGMEADIFAVSASDA
                     DNNLESKNVDVLLLGPQVRFMKAQFEQKLAPKGIPLDVINMQDYGMMNGEKVLAQAEN
                     LMK"
     gene            6810..7130
                     /locus_tag="ACG842_001979"
     CDS             6810..7130
                     /locus_tag="ACG842_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HFQ0248511.1"
                     /translation="MEDQQNLEAIMGLIMFGGNAKSDAMEAIAAAKIGDFELADRKIA
                     DAEESLIQAHHAQTDMLTQEAQGNHMQVTLLTVHSQDHLMTSIAFTDLAKEIIELYRR
                     VYSE"
     gene            7238..8698
                     /locus_tag="ACG842_001980"
     CDS             7238..8698
                     /locus_tag="ACG842_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFQ0248512.1"
                     /translation="MNGLSSWMEKYILPVAAKIGAQKHLVALRDAFIGILPATMAGSV
                     AVMINAIIRDLPQQFNSTYADSLAADKGIFAVLNVIIGINGFVWNGTLAIAGLIFAFS
                     WGYNLARAYGVNDLAGGIVGLATLIQGISFGPALSAALDVNVPSNVADAINSSEIGAT
                     IADGTLSVGLWGWLKLDHLNGNAYFTVMIMGAISVIIFCKLMLANITIKMPDSVPPAV
                     SKAFAAILPATIALYVIAIINYIVGRVADGQLIIDLVQKYIAEPFLGLSQGLGAVLIV
                     TVFVQVFWFFGIHGSNVLAPVLEGIWGQAQLINIDIFQKGYNGKTGTPAVLDAIEDGK
                     AYMWVRGSFDAFAWFGGSGGTIVLLIAILIFSKRADYLTVAKLSIGPGIFNINEPVMF
                     GLPIVLNAIMFIPFLIAPVVATAIGWTATYLGLVAPVSQQVTWVVPPFLLSFLATGAD
                     WRAPIVTLVCMVVTFLIWAPFVISANKMDPAAGEQQ"
     gene            8766..8987
                     /locus_tag="ACG842_001981"
     CDS             8766..8987
                     /locus_tag="ACG842_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0248513.1"
                     /translation="MITIASAMQQDEIKELLEGYNEEGMTFTFKEKQGIKLFFETSAE
                     DPEVAAKKAKELIKAQPWGTVLYFQSTAV"
     gene            9018..9200
                     /locus_tag="ACG842_001982"
     CDS             9018..9200
                     /locus_tag="ACG842_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3188 domain-containing protein"
                     /protein_id="HFQ0248514.1"
                     /translation="MIKNGLFLCSIGSLIVLYSLNPHSQRYDLYSMVTGLFLTGLGIY
                     FFMKGKKREEAKEKNN"
     gene            9200..9613
                     /locus_tag="ACG842_001983"
     CDS             9200..9613
                     /locus_tag="ACG842_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3284 domain-containing protein"
                     /protein_id="HFQ0248515.1"
                     /translation="MEITKKLNIPASFFYDKVMDSVLFDVRKATGKSVTRKQLKNLEY
                     VKQFSQNNRARIKIEEVIENQSYKFRTSTTKNEFVVHYQIKPLDDSTCEVHYTEKMES
                     YGMLQKLNDMLLGTILMYFKRRRFKKMLDMIEATY"
     gene            9736..10917
                     /locus_tag="ACG842_001984"
     CDS             9736..10917
                     /locus_tag="ACG842_001984"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0248516.1"
                     /translation="MTNKVRELGVSSIEEMFDLATYAFNGADTPERRERFRTLAENSW
                     NYGFFDEEDRLTSQVMATPFPVNFYGQEYLMAGIGYVASYPEARGQGGINQIMEKILE
                     DCRDRKVSLSYLAPFSYPFYRRYGYEQSFDKISYQLNSRDVPFIKKKSGKIKRMDFED
                     AKAAIRRIYEQMPENQRAGLKREDWWYTYKFKQKGNYQFALHFDDNEEATGYIVYQLA
                     TSSFIIAEWGFLDHDAFCSLVRFVSSHNGAFETFEYSCGYGGNDQNYLLENPFASVMI
                     TPYMMARIVDISLFLENYPFKKRQAAFALQIEEDHYAKWNEGIVEVTIENGKNHLSKV
                     EQTKLPIVRGSIQHITQLLLGYRKVEELVFQERIQVTKEYEETLSSILPQQQPILNDY
                     F"
     gene            complement(10988..11152)
                     /locus_tag="ACG842_001985"
     CDS             complement(10988..11152)
                     /locus_tag="ACG842_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0248517.1"
                     /translation="MNDKPKEKVYAERLDSSKKNREKITKEAIQDHKKENPKTKENKK
                     FKFTKKEDLL"
     gene            11306..11379
                     /locus_tag="ACG842_001986"
     tRNA            11306..11379
                     /locus_tag="ACG842_001986"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11340..11342,aa:Arg,seq:cct)
     gene            complement(11448..12587)
                     /locus_tag="ACG842_001987"
     CDS             complement(11448..12587)
                     /locus_tag="ACG842_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFQ0248518.1"
                     /translation="MARLVKRGNSWQYEISYKKDDGKYTKIRKSGFKTKGEAKDAANE
                     LEYNLNKGLKGDRKNLLLSDYFEDWMQLYKEGTVSPITYRKYEDTLMNIKKYMPAVLI
                     SDLDRVGYQRFLNKYAKDHVKSTVIKFNNHIRASLKDAVEEGLIPFDPTRKAVIKGKD
                     SLKPKEDKYLDYDQFKSLMKLVEENLSAQYSSPMLVLVAGATGMRFAELLGLTWEDID
                     FEDQIITINKTWNYKLNEWGKTKNETSNRKISIDKHTIDLLKKFKINQKELFENFEIK
                     NPHNFVFFNLKNGLVSSNAVSKYLRKKLKELGIEKQFTLHGLRHTHASILLYQGVNIL
                     SVSKRLGHSSLETTMSTYLHIVRELEDQDKEKINTVFDSLYKNDN"
     gene            12759..12893
                     /locus_tag="ACG842_001988"
     CDS             12759..12893
                     /locus_tag="ACG842_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0248519.1"
                     /translation="MIKVIFKRITELPEFRLFVDYWYVWFFIVALLIGLDVILERKLK
                     "
     gene            complement(12886..13521)
                     /locus_tag="ACG842_001989"
     CDS             complement(12886..13521)
                     /locus_tag="ACG842_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0248520.1"
                     /translation="MDNSNEKLTKKEYLELIVEGGIGAIPYIGGVVQSLYFGSKNEKR
                     FKRIEKFYKNLNESLENIRDQIPEDVFNLENKDQLIGIFEGINDEVEKSKAQNKIDMY
                     KNLYKNCLLRINNASWDNEEYYLLVLNQLTSIEIQLLACLMSRGNENFTGNISMSGYS
                     QELIDGSLNRLSDFGLLEKTISSIVLGDVGKQNMGYKISHLGIQFINSTLT"
     gene            complement(13634..14269)
                     /locus_tag="ACG842_001990"
     CDS             complement(13634..14269)
                     /locus_tag="ACG842_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3221 domain-containing protein"
                     /protein_id="HFQ0248521.1"
                     /translation="MDGIFGFTGIVLFIIGLIMLIVRFIKKTNKKTPMILLIIGIIFT
                     TVGFSLSSSNEANDNNKQESSSSDTQTTTSNSSNKSLTQFEEFLETNNHDWGAFLDSY
                     YSITPATEQNTAFTKYISGKTYTFEGTVIESMTTRIAIIADKEYDDKSWNDISATPKV
                     SYVIFAKDLNNADTFTKGDKVTFTGEISSRGSNIEKSYAQWDMINSKVSKK"
     gene            complement(14303..>14416)
                     /locus_tag="ACG842_001991"
     CDS             complement(14303..>14416)
                     /locus_tag="ACG842_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HFQ0248522.1"
                     /translation="ETQISSADEIKKYKELLDEGIINQEEFDFKKKELLGL"
BASE COUNT         4620 a         2433 c         2857 g         4506 t
ORIGIN      
        1 gggatctatt tttcagataa caacttatat gaagtgtctg cagattcgtc ataaagagtt
       61 tgttcgggat aagaaaagat aagataaaaa ccatactgat cacgtacgac taaacgatat
      121 tcatctattt ctaccacaga tgtgccaaga gactgaccgt tgatcaaaaa cgtgtaatca
      181 gatgtaatct gtaattgttg tttttgctgg ctatcactaa agaaccatgt accttgatac
      241 tttgagagtc cttctgactt ttccgcttgt tttttctttt tcaagtaaat aaaacttgag
      301 agaacggtaa tgagtgaacc aagaaaatag atcatttttt ttatcaccaa atcactcctt
      361 tatgagaaaa taatcgcata gacatattct ttcttcttta tttcattgta ccgaataaaa
      421 gctaaaattt ctctaagact ttcgactggt ttttttactg tttttctaca aattatgata
      481 ggatacatag taggaagaaa ggtgacataa tttgaagtat cagcaagtgg aaatagcttt
      541 ttttattgac cgcccgaatc ggtttatagc acattgtatg aaaaaagacg gagaaataat
      601 tattgcgcat gtcaaaaata caggtagagg aaaagaggtc ctgataccag gcgctgaagt
      661 ggctattgta tttgctcctg gacctaaacg gaaaaccgcc tatgatctga ttgctgtaaa
      721 aaagaagggt gactggttta atatcgatag tcaattgcct aatcggctgg caattgatgg
      781 tattttagac ggcacgatcc ttttaccagg attaactggt ccatttacgg tatacaagcg
      841 tgaagtaact tatcttcact ccaaatttga tatttattta gagacggtgg tcggacaaaa
      901 agcatttgtt gaagtcaaag gtatgacact tgaaaataaa gcgattggtg cttttcctga
      961 tgcgcccaca ctcagaggat tgaaacatat aggagaatta acgtatgccg cacaagatgg
     1021 ttatgccgct tatgtcttgt ttattgctca gttcgaacat cttcatctgg caacgatcca
     1081 tgaagaaatg caaccagcac tagctgatat ggttcgacat gcgcagcaaa gcggagttca
     1141 aatcctcgcc tataattgtc aagtatcggc agatgaggta actgtcaagc aggccattcc
     1201 atttgatgta gatatccctt ttgaagatcc tatacaatag aaagaaggta aaataatgat
     1261 caacttaggt attatcggaa caaattggat tactcatcaa tttgtccaag cagctttaag
     1321 cacaaaaaaa tatgaattga cagcggtata ttctagacat ttggaaaaag cacaggaatt
     1381 tggttctcat tatgaagggg atatcgaatt cgcaacagat ttgaagactt ttttcaatat
     1441 tactcatatg gacaccattt atattgcttc tccgaatagt cttcacttta gtcaagctaa
     1501 gcaagcaatt ttggcaggaa aaaatgtgat agttgaaaag ccagcttttt cgacgccaaa
     1561 agaaatggat gaaatcatcc atttggcaaa tgaacacaat gtgtattttt ttgaagcagc
     1621 aagaaatatc catgaatcag gttttaaaaa gattgccgat tacttaccat taaaagacga
     1681 gataatcggt gcaaatttca gctatatgaa atattcttcg cgctatgacc aagtattaga
     1741 tggagaagaa ccgaatatct tctcgactca tttctcaggt ggtgcattga tggatttagg
     1801 tgtttacctt gtatatgcag ctgttgcttg gtttggaatg ccaaaagaag tccattattt
     1861 cccaagaaag atttctactg gtgtagatgg tcttggatgg gggatcttac gttatgagaa
     1921 ttttgatgta tcgatccagc caggtaaaat cggtgattct tatttaccgt cagagattta
     1981 ttttgactca ggaacgctag tgatgaatgg agtcaatgca attgaacaag cacaatttca
     2041 tgatcgcgcg cataaagaaa tcattgattt ggcggtaaca gctgcagaaa atccaatggc
     2101 agatgaagca gaggattttg ccaatgtcat ggctcatccg aaagatccag cttggggatt
     2161 gttgtatgaa gagtggatcg agctagctag aaatgtgaac caagtgattt atgatttaag
     2221 aaaaaatggc ggcatcaaat tcgatgcaga taatgaggaa gatttttaat gaacgaattt
     2281 gtacaagtat ttcccgaaaa ttggcagaaa aaatatgagg ataaagggtt tatctcacct
     2341 tctcttattc aacgagcagt ttttcagcca ctaagtaata aacaaagtat agtagcggtt
     2401 tctcctacag gttcaggtaa aacactggct tatctttggc ctctactact aaacgtggaa
     2461 ccaggtgaag caagtgcgtt ggtcattttt gcttcttctc aagaactagc tattcaagta
     2521 gcagatgttg cacgtgaatg gggaaaagac aaagagttga aagtccaatc tttggtgggc
     2581 ggagccaacg tcaaacgtca gattgagggt ttgaaggaaa aaccagaaat cttgattggt
     2641 acaccaggaa gaatattgga attgatgaaa gcaaaaaaaa taaaagcaca tcaagtgaaa
     2701 acaatggtat ttgatgaagc agaccagcta tttgacgctg ggaacagcca aatcattgat
     2761 caaattcttc atcaagctcc aacagagtat cagctagctt ttttttcagc gacagcagat
     2821 cgttcccttg aaagcattga aaaaatcact gggaaaacgt ttgagacaat tgacgtgaca
     2881 gcagaggacg attctcgaaa aggactgaga cattactttt tacgtgtacc gatacgaaag
     2941 aaagatgact acttgagacg tctcacgcat atagaagatt ttcatggtct gattttcttt
     3001 aatcaactga ctgaattagg cgtaatggaa gaaaaattgc tgtatagagg tgtaccggta
     3061 ggaagtctgg cctctgatca gaacaagcta ctccgaaaag cagcgctaga acaattcaaa
     3121 aagaaaatgg tcagagcact tttgaccaca gatgtggcgg ctagaggttt agatattaca
     3181 ggtcttcctt atgtagtgaa tgcagaagtt ccgttaagtg aagaagctta tttgcatcgt
     3241 tcgggaagaa ctggtcgaat gggtaatgaa ggaatagtaa ttacactcgt acaagataat
     3301 aatctaagag atttaaaacg gcttttacgt ccatcaaata taacacttga agagattttt
     3361 ttatacggag gtaagctgca aactgagcag ccaaccaaag aagatgcagc tcctgcagta
     3421 aataaaaagt atgcgaaaac tttttctgga aaggagcaga acccagaata taacaaagaa
     3481 aaaataagaa aaaaagttaa aaataaatca aaaaaagaga gaaataaagg agcaaggcgc
     3541 aaataagcgc ctttttatgc tatgattatg aaaacgggaa caaaattggt tgccagagga
     3601 ggggttatgt gaaagaacgt caagaagaga tcttgcagct attgacagaa aaccaacaag
     3661 gattgacagc aagtgatgta gctgagcgct taactatcga tcgaagcaat gcaagccgat
     3721 atttaagtga actatacaag gcccatcata ttgtaaaaac cgctggccga ccagtcgttt
     3781 actctttgcc aacggaaaag tcaaaatctg atgaagtgca tgtcgactct tctacgcaag
     3841 tgacttttga aacactcgtt ggagaaaatg attctttaaa agtcagcata cagcaggcaa
     3901 aagctgcgat tctttatcca ccgcgggggt tgcatacgat tatttttgga gaaactggta
     3961 cagggaagtc gatgtttgct gaatgtatgt atcattttgc aatcgattca gagatgcttt
     4021 ctgcagatgc gccatttgtt tctttcaatt gtgcggatta tgatcaaaat ccgcaactgc
     4081 tctttggaca catctttggg atcaaaaaag gggcttacac aggagcagct caagatagtc
     4141 ccggtctaat agccaaagca gacggcggta tattgttttt agatgagatc caccgtttac
     4201 cgccggaagg gcaggaaatg ctgtttactt tcattgataa aggtatttac cgtccgcttg
     4261 gagaaagtgc acaagtacat gaagcatctg ttcagatcat tggggcgacg actgaatctt
     4321 cagaaagttt cttatctacg ttcaatcgac ggattcctat ggcaatcacc ttgccgaatc
     4381 ttgcttcacg ttcactggat gagcgttatg aaatcatctc tctttttatc aaacaagaag
     4441 cgaatcgttt gaatcaacgt atcgatatcg aaaaagaagc gattcttgcc ttcatgcttt
     4501 atgatgcgga aggaaatatc ggtcaagtga aacgagactt gaaactagtc tgtgcaaaat
     4561 cttttttaca ttaccgtact catcatgaga aaaagctaat cattcgaaaa gaagaacttt
     4621 cccttcaagt acaaaaagga ttactgaaaa tcaaggaaat accggaacgt ctcgaccgat
     4681 tcatggacag caaaagtcag tatttgacat ttgaaccagg atttgctgat gttgtctggt
     4741 cacaagatcc agaacgaaac atgcaggtct ataacgatat tgaggaaaaa gtcacttctc
     4801 tttccgaaac cggtgtggag aacattgatc tagagaccct catttcaaaa gatgtggatg
     4861 cttatttcca gacatatgtc aaagaattga ctaaaagttc gattcccaaa gatcttttgc
     4921 cagatgaaat ctggcggtta accaatcaat tgtatgatgt ggctgaaaaa cggttagacc
     4981 gtatttataa tgaaaaagct cgttttgctt ttgctttgca tctgcaaagt acattggatc
     5041 gtgtgagaga aggtcatatg atcgtccacc cagatttgaa taacgtacgg aaaaatttta
     5101 agagggaatt ccaggtagca atcgatcttt caacgatcat tgaagaagaa gaacaagttg
     5161 agattccttt tgatgaaatc ggctttatta gtatgtttct ttctatcaat ctaggagaaa
     5221 gtgagcctgt taaagaaaac aaagtagatg ttctcatatt gatgcacggc agacagactg
     5281 cttcaagcat gttggagatg gcacaagaac tattaggaac aaaagtaggg aaagctttta
     5341 atatgccact ggttatggaa gttcaaacaa tgtatgaaga agtgttacaa tatgtcaaaa
     5401 actatcaaga gcatttgacg aatggcctac tcttgttaac agatatggga tcattaaaca
     5461 ccttcagtga tttgatttat gaagagactg gcattcggac gaaagctatc acgatgacaa
     5521 gtaccatgat tgtgatagaa gcgatacgga tggcaagtgt tggaagaagc ttagaagata
     5581 tttatcaaaa tatccagctt tcctttgaat cgatcgtacg tgatcagttc aaagcggaaa
     5641 agtctgaaac tttacagcgt aaaaaagccg taatcgtgac atgctttact ggtgaaggag
     5701 tagctgccaa attatatcag cgtattatgc cagtgattaa tcaaaataag gtagaactga
     5761 tccagatgca gtttttagaa cgtgaatcat tcaaaaaaca catcgatgcc ttattggaag
     5821 aatatgagat caaagcaatt gcgggaacag ttgatgttga ttatcaaaat atcccatttt
     5881 tttcagccta tgacgttttt gatgatgaaa aactaaatat tttaaagcgt atagctgatg
     5941 atgagatccc gacagctaaa attgtcgatt cacttgctgg atcattaagc gatgtctttt
     6001 ctattgatga actgatccgt tcattgcaaa agacagttca gcagatccag accgatttgt
     6061 cgattatcgt ggaacctgga gtagatactg gaatcgttat ccatttagcc ttcatgttag
     6121 acggattgat caaacaagaa agccatcggc aatttgagaa actgtctatt ttctcaaaaa
     6181 tttatcgttt ggaaatggat atcgttcggg caaacttgat ggcactagaa cgaaaatatc
     6241 aggtaaaaat tccagaagat gatatcgcat ttcttacaca aatgtttctt gaaaataaaa
     6301 taaaaaaatg aacacactaa tcatactgtt tacacactaa acatctgtgt gcgcactata
     6361 aaaaagagct aaaaagcatg aaaacgctat ttcagctctt ttttttattg gcatgcttct
     6421 tgcgttatat aaaagtgtaa aaaaataaaa ggagtgttaa aaatggcaaa aaaaacaatc
     6481 atgttagttt gttcagcagg gatgagtacg agtttattgg tcacaaaaat gcaaaaagca
     6541 gcagaagaaa aagggatgga agccgatatt tttgcggtct ctgcctcaga tgcagataat
     6601 aatttagaat ctaagaatgt cgatgtatta ttattaggtc ctcaagtacg tttcatgaaa
     6661 gctcaatttg aacaaaaact tgcgcctaaa ggtattccat tagatgtcat caatatgcaa
     6721 gactatggca tgatgaacgg agaaaaagtt cttgcacaag cagaaaatct aatgaaataa
     6781 atcaagtatg cagttggggg tataacaacg tggaagatca acaaaatcta gaagccatca
     6841 tggggttaat catgtttggc ggtaatgcaa agagcgatgc aatggaagcg attgcggctg
     6901 ctaaaatcgg tgattttgag ttagcggata ggaaaatcgc tgatgcagaa gaatcactta
     6961 tacaagcgca tcatgcacaa acagacatgt tgacacaaga agcacaagga aatcatatgc
     7021 aagtgacatt attgactgtt cacagtcaag atcacttaat gacatctatt gcgtttactg
     7081 atttagcaaa agaaatcatc gaactttatc gtcgcgtata cagcgaataa atacaacttc
     7141 ttttttgaac ggaagaagaa aaatttttga acggattttt tattttgtta taaggagaac
     7201 tctctttata taaaaaactt ataggaggaa gtaaaaaatg aacggactat caagttggat
     7261 ggagaaatac atcctgccgg ttgccgcaaa aatcggagcg caaaaacatt tagttgcttt
     7321 gcgtgatgct tttatcggca tactgcccgc aacaatggcg ggatcagtcg ctgtaatgat
     7381 caatgcaatt attcgtgatt taccgcaaca gtttaacagt acctatgctg acagtttagc
     7441 agcagacaaa ggaatctttg ccgtattgaa tgtcatcatc ggaatcaatg gttttgtctg
     7501 gaacggtaca ctagcaatcg ccggattgat ctttgccttt tcttgggggt ataatcttgc
     7561 ccgagcttat ggagtaaatg atctagctgg ggggatcgtt ggtttagcta ccttgattca
     7621 aggtatttcc tttggtccag cattatcagc agcattagat gtgaatgttc cttcaaatgt
     7681 cgcagatgca atcaatagtt ctgagattgg tgcaacgatt gcagatggca cactgtcagt
     7741 tggattatgg ggttggctaa aactagatca tttgaatgga aacgcttatt ttacagttat
     7801 gatcatggga gctatttcag tcattatttt ttgtaaattg atgcttgcca acattacgat
     7861 caaaatgcct gattcagtac caccagcagt atctaaagca tttgcagcca ttttaccagc
     7921 gacgatcgct ttatacgtca ttgctattat caattatatc gtaggtcgtg tagctgatgg
     7981 gcaactgatc attgatttgg tgcaaaaata tattgcagaa ccattcctag gattatctca
     8041 agggttggga gcagtattga tcgtcacagt atttgttcaa gtattctggt tctttggtat
     8101 tcatggatca aatgtattgg caccagtgct agagggaatc tggggacaag cacaactaat
     8161 caatattgat attttccaaa aaggttataa cggaaaaact ggtacaccag ccgtgttaga
     8221 tgcaatcgaa gatggaaaag cttatatgtg ggtccgcgga tcgttcgatg cttttgcatg
     8281 gtttggcgga tcaggcggaa cgatcgtctt actgatagct atcttgattt tctctaaacg
     8341 tgcagattat ctaacagtag ctaaattatc cattgggcct ggtatcttca atatcaacga
     8401 accagtgatg tttggtctgc caatcgtatt gaatgcaatc atgtttatcc cattcttgat
     8461 tgctccagta gtagcaacag caattggatg gacagctact tatcttggcc tagtcgctcc
     8521 ggtatcgcag caagtcacat gggtagtacc gccgttctta ttgtcattct tagcaacagg
     8581 agcggattgg cgggcaccga tcgtaacatt agtatgtatg gtcgtgactt tcttgatttg
     8641 ggcaccattt gtcatctctg caaacaaaat ggaccctgcc gcaggcgaac aacaataaga
     8701 tgaacgtgag gagcgggacg ctttaagttt cgttcctcat aatgaatttt cgaggaggaa
     8761 ctgaaatgat tacaattgcc agtgcaatgc aacaagatga aatcaaagaa ttgttagaag
     8821 gatacaatga agaaggaatg acttttacat ttaaagaaaa acaaggaatc aagctgttct
     8881 ttgaaacaag tgcagaagat ccggaagttg cggctaaaaa agcaaaggaa ctaatcaaag
     8941 cacagccttg gggaacagtc ctttattttc aatcaacagc tgtttagaaa atggtttaaa
     9001 aagataggat ggttgagatg ataaaaaacg gtttattttt atgtagtatt ggttctttga
     9061 tcgtgttgta tagtttgaat ccacattcgc aaaggtatga tttgtatagt atggtcacag
     9121 gtctttttct aacaggatta ggaatctatt tcttcatgaa ggggaaaaaa agagaagaag
     9181 caaaggagaa aaataactaa tggaaatcac gaaaaaatta aatattccag catcgttttt
     9241 ttacgataaa gttatggact ctgtcctttt cgatgtacgt aaagcgaccg gcaaatcagt
     9301 aactagaaaa cagctgaaaa atttggagta cgtcaaacaa ttctcgcaaa ataaccgggc
     9361 tcgcataaaa atcgaagaag tcattgaaaa ccagtcttat aagtttcgta catcgacaac
     9421 gaagaatgaa ttcgtcgttc attatcaaat caaaccattg gacgattcga cctgtgaggt
     9481 tcattacaca gaaaaaatgg agtcttatgg catgcttcaa aagttgaatg atatgctttt
     9541 aggaacaatc ttgatgtatt ttaaacggag acgtttcaaa aaaatgctag atatgatcga
     9601 agcaacgtat taaatcaatg aaacatggta ctatttagtt attgaaaaat gaagtaagct
     9661 agaaggaatt tttcatagga agatagatct ttcctgcgaa tcctcactag cttttttgta
     9721 ggaaggggca gcaaaatgac aaacaaagta agagagctag gtgtatcttc gattgaggaa
     9781 atgtttgatt tagccactta tgcatttaat ggggcagata cacctgaaag gcgagaacgt
     9841 tttcgtacac ttgcggagaa ttcttggaat tacggatttt ttgatgagga ggatcggcta
     9901 acaagtcaag tcatggcgac cccttttcca gtgaattttt acggacaaga gtacctaatg
     9961 gccggcattg gctatgttgc ttcttatcca gaagcaaggg gacaaggcgg gatcaatcag
    10021 atcatggaaa agatccttga agattgccgt gatcgtaaag taagcttatc ttatctggcg
    10081 ccattctcct atcccttcta tcggcgctat ggctatgaac aatcatttga taaaatcagt
    10141 tatcaactaa acagtcgtga tgtgccgttt atcaaaaaaa aatctggaaa aataaagcgt
    10201 atggattttg aagatgcaaa agctgcaatc agaagaattt atgaacagat gccggaaaac
    10261 caacgagctg gtctgaagcg cgaagactgg tggtatacgt ataaattcaa gcaaaaaggc
    10321 aattaccagt ttgctttgca ttttgatgat aatgaggaag caacgggata tatcgtttat
    10381 cagcttgcaa cgtcttcctt tattattgcg gagtggggat ttttggatca tgatgctttt
    10441 tgcagccttg ttcgcttcgt aagttctcac aatggtgctt ttgaaacatt tgaatattca
    10501 tgcggctacg gcggtaatga tcaaaattat ctattagaaa atccatttgc ttctgtaatg
    10561 atcacacctt atatgatggc gaggatcgta gatatttctt tgtttttgga aaattatcca
    10621 tttaaaaaga gacaagctgc atttgcccta caaatagaag aagatcatta tgcgaaatgg
    10681 aatgaaggaa tcgtggaagt aacaatcgaa aatggaaaaa atcatctctc aaaagtagaa
    10741 caaacaaaac tcccaatcgt tcgaggaagt atccaacata tcacgcagct attattaggg
    10801 tatcgtaaag tggaagagtt ggtattccaa gaaagaatac aggtgacaaa agaatatgaa
    10861 gaaacgttaa gctcaatctt gccacagcaa cagccaattc ttaatgacta tttttgataa
    10921 aaaacgggtg atgacaatat ttttgtcacc acccttttgt ttttcatttg gcatttgaag
    10981 gaaaatgtta aagtaaatcc tcttttttcg taaatttgaa ttttttgttt tcttttgttt
    11041 tagggttttc ttttttgtgg tcttggattg cttctttcgt aatcttttcc cgattttttt
    11101 tagatgaatc taagcgttct gcgtatactt tttcttttgg cttatcattc attggaaagg
    11161 acctccctac attatttcat tctacctcta gtttatcatc tgttaaggac tagtggaaac
    11221 gttttgccta acttataagc tgattttttt agcagataag cttgcaagta aaaaacaaac
    11281 aagctacaat tgatttattg aaattgttcc tgtagctcag taggatagag caatcgcctc
    11341 ctaagcgata ggtcgctggt tcaattccag tcaggaacgt ataaataacc cctgtatcct
    11401 ttgcggtaca ggggtttgtt ttgtatttgt gccaaaaatg tgccaaacta gttatcattt
    11461 ttatataaac tatcgaacac agtattgatt ttttctttat cctgatcttc aagctctcga
    11521 acaatatgaa gataagtaga cattgtagtt tctaaactgc tatgtcctaa acgtttcgat
    11581 acgctaagta tatttactcc ttgataaagt aaaatagatg catgtgtatg ccttagtcca
    11641 tgcaaagtaa attgcttttc aatccctaat tcttttaatt ttttgcgcaa atatttgctg
    11701 acggcgtttg atgaaactaa tccatttttt aagttgaaaa aaacaaaatt atgagggttt
    11761 ttgatttcaa aattctcgaa taattctttt tgattgattt taaacttttt taagagatca
    11821 atcgtatgtt tatcaatgga aattttccta tttgaagttt cgttttttgt ttttccccat
    11881 tcatttaatt tataattcca tgttttatta atagtgatga tttgatcttc gaaatcgata
    11941 tcttcccatg ttaatcctag aagttcagca aatcgcattc cagtggcacc agcaactaac
    12001 acgagcatag gagaagagta ctgcgcagaa aggttttctt ctacgagttt cattaaagat
    12061 ttaaattgat cataatctaa atatttatct tcttttggct tcaatgaatc ttttcctttg
    12121 attactgctt ttctagttgg atcaaacgga attaatcctt cttctacggc atctttcaac
    12181 gacgctctaa tatggttatt aaacttaata acggtagatt ttacatggtc tttagcatac
    12241 ttatttaaaa agcgttgata tcccactcta tctaaatcag aaatcaatac cgctggcata
    12301 tatttcttta tgttcattaa cgtatcttcg tattttctat aagtgatagg agatactgtt
    12361 ccttctttat aaagttgcat ccaatcctca aagtaatctg ataataatag atttttgcga
    12421 tcccctttaa ggcctttgtt caagttatat tctaattcgt tggcggcatc ttttgcttct
    12481 ccttttgtct taaatcctga ttttcttatc ttcgtgtatt ttccgtcatc ttttttgtat
    12541 gaaatttcgt attgccaact atttccacgt ttgactaatc ttgccataat tgaatcagct
    12601 ctctttctct gatacaatag gaactataaa gaagcccatt gtttgggttt tattattttt
    12661 tgcacatcct ttttgcttgc cggcgcaagg atgtgctatt tgtcgtttta atggtataat
    12721 tggatgtgta ttagatcatc caatcagagg tgtttattat gattaaagtg atttttaaaa
    12781 gaataactga acttccagag tttagattat ttgttgatta ttggtatgtc tggtttttta
    12841 ttgttgcatt gctgataggt ctagatgtca tattagaaag aaaacttaag taagagttga
    12901 gttaataaat tgaattccta aatgagatat tttgtagccc atattttgtt tgccgacatc
    12961 acctaatact atggagctaa tcgttttttc taacagacca aaatcagata atctatttaa
    13021 gctaccatct attagctctt gtgaatagcc agacatgctg atatttccag tgaaattttc
    13081 gtttccacga ctcattaagc atgctaacaa ttgaatctca atcgatgtta attgattcag
    13141 tacaagaaga taatactcct cgttatccca tgaagcgttg ttaattcgaa gtaaacaatt
    13201 cttataaaga tttttataca tatctatttt attttgtgcc tttgattttt ctacctcgtc
    13261 atttatccct tcgaatattc caatgagttg gtccttgttc tctaagttga aaacatcttc
    13321 tggaatttga tcgcgaatgt tttctaatga ttcgtttaaa tttttgtaga atttctcgat
    13381 gcgtttgaaa cgcttctcgt ttttagaccc aaaataaagt gactgcacaa cgccaccaat
    13441 ataaggaatt gctccaatac cgccttcaac tattaattca agatattctt ttttggttag
    13501 tttttcattg ctattatcca tagttacctc ctattttgtc atttccttta caagcattgt
    13561 gactcctgat ggcactcttt tgtataggtt ttattattgt gcacaatcta actcttagtg
    13621 gggcgtggtt catttatttt ttacttactt tactattaat catatcccat tgagcatagg
    13681 atttttctat gttcgatcct cttgaactaa tttctcctgt aaaagttact ttatcgccct
    13741 ttgtaaaggt atcagcattg tttaagtcct tcgcaaaaat tacatatgaa actttaggtg
    13801 ttgcagaaat atcattccaa cttttatcat cgtactcttt gtctgctata atggctattc
    13861 tagtagtcat tgattcgata acagttcctt caaaagtata tgttttacca ctaatatatt
    13921 tagtaaaagc tgtattttgc tcagtagctg gagtaataga ataataacta tctaagaaag
    13981 caccccagtc atgattattt gtctctaaaa attcttcaaa ttgagttagg cttttattgc
    14041 tgctgttaga tgtagtagtt tgagtatcag atgatgagct ttcctgtttg ttattgtcat
    14101 ttgcctcgtt tgatgaggaa agcgagaaac ctacggtagt aaaaataatc ccgataataa
    14161 ggagaatcat aggtgttttc ttatttgttt ttttgataaa tcttacaatt aacatgatta
    14221 aacctataat aaataaaaca ataccagtaa aaccaaatat accgtccatt aatagatcct
    14281 ctttctcttt tgatattgaa tattataaac caagcaattc ttttttctta aaatcgaatt
    14341 cttcttgatt aataatccct tcatctaaca actctttata ctttttaatt tcatctgcac
    14401 ttgaaatttg tgtttc
//
LOCUS       DAZKLM010000070        13978 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3764
            SAMEA115706913-rid22582563.denovo.072, whole genome shotgun
            sequence.
ACCESSION   DAZKLM010000070 DAZKLM010000000
VERSION     DAZKLM010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706913
            Sequence Read Archive: ERR13196647
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13978)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13978)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287823.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287823.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,004
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,849
            CDSs (with protein)               :: 2,849
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 41 of 96
            Pseudo Genes (incomplete)         :: 52 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 15 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13978
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706913-rid22582563.denovo.072"
                     /isolate="VRE3764"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            140..460
                     /locus_tag="ACG842_001992"
     CDS             140..460
                     /locus_tag="ACG842_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0248523.1"
                     /translation="MKKRLLWCLLFLSLSLTSQPVFAEETQRSPITGTRLYKNVYVQT
                     TDLDSAIPETEERSLQPRFGYFWYCPTCDYESCWHFLHSTAAAFAFTHTQKYDGHHPV
                     IYEV"
     gene            912..1112
                     /locus_tag="ACG842_001993"
     CDS             912..1112
                     /locus_tag="ACG842_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFQ0248524.1"
                     /translation="METGTVKWFNSEKGFGFITAENGNDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEEGQRGPQATNVTKA"
     gene            1259..4048
                     /locus_tag="ACG842_001994"
     CDS             1259..4048
                     /locus_tag="ACG842_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase C-terminal domain-containing protein"
                     /protein_id="HFQ0248525.1"
                     /translation="MKPRTYAVVDIETTGTNPKEDRIIQFGCVMIESGRITSRFSIDI
                     HPGRKISKQIQHLTGITNQRVQKAPYFEDVAQTIYNLLADTIFVAHNIYFDYNFLNHE
                     LMRCGLPSLKIPGIDTVELAQIFLPTEPSFRLADLSESLGFIHENPHQADSDAEVTGQ
                     LLLLIEERMRKLPIITLEQIARLSRHTGMDTSRFIYHVIEEMKEKPEPLDPSLEIVDG
                     LALQKKEVELFTSVHYGERTYPRKKQAKEKMFGKTLMYRKEQNRLMNAVYDHFTKDES
                     KDLMIEAATGMGKTIGYLLPLSYLATPEKPAVISTVSLVLQQQIIEKDIPLLNQLLDQ
                     PIQPVIIKSYRHYIDLQRFKGTLVEPPEQKQYALYQMAVLVWLTQTKTGDLDELHLTN
                     LNHSFFADIAHRGIGFLARNQSFYEQDFVRFLQKKIRQSNFLIVNHAFLIQETQRKEP
                     LLPASPFLLIDEVHHLPEIAEKVNDKRLSINYFHKQIQQLEEPNQLFDRIQSYLPKEH
                     EALRWLSIYREELSALCEAQICMAEGLMSLVSGKQPNGQYPEEVMMTKEMLDQLPLES
                     GTAIDHLQLFYQEIQQLQSQMRLTFEIIQESWTNQMRIDLASLYSLFDQLDKQYLLMD
                     QWLEDWRDHLIHWFVPGNTAQQMIFHLHDFQAAMLPSTVWYPRYDRILYIGGTLKIGP
                     NRHYLSKRLGIEAAPLKVIASPYDYEKQARIYVPKEAPAVHQVSNQAYIDYLETALTG
                     LIEQEDRPILVLFTSHDVLQKVYQKMHLSMLEKGREILAQGFGGSREKLLKRFILSEK
                     SVLFGADSFWEGVDLPGDALQIVVVTRLPFDNPLRPMVKARNAYLESEGINPFYQESV
                     PKAALKLRQALGRLIRTETDRGVLLILDRRLVTAKYGARIIHALPKKVPIKELAMEEI
                     YQDIHEFLKKNEEGS"
     gene            4095..4622
                     /locus_tag="ACG842_001995"
     CDS             4095..4622
                     /locus_tag="ACG842_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5590 domain-containing protein"
                     /protein_id="HFQ0248526.1"
                     /translation="MKIKERRPLKKKQTKEKTINRVLLGLIVFLLAVIVISSIFYLRS
                     SRPAVQAKKEAVEIAKKYANLEEADHFYWFTRKKTYFSLTGTNDKGKEIAVIIPKSGE
                     KVKVLDQTKGLTENEAKQTIAAAHPEIQIEKAALGMYDDQPVWEVVGKETNGKLNYYL
                     IGFESGDEVKTIKGI"
     gene            4643..5833
                     /locus_tag="ACG842_001996"
     CDS             4643..5833
                     /locus_tag="ACG842_001996"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HFQ0248527.1"
                     /translation="MELSKRTKRLEPSVTLAAAAKAKALKAKGRDVLSLTVGEPDFAT
                     PENIQEAAIEAIRNGKASYYTPTAGIPELRQAIVDYLKKNYGLVYEPSQTVVTDGAKF
                     ALYTLFQTILDPQDEVIIPVPYWVSYGEQVKLAEGLPVFVKGEESNSWKVTVEQLEQA
                     RSEKTKAVIINSPSNPTGMIYSKNELQAIGEWAVKNDILIVADDIYGRLVYNGNEFTP
                     IATISEAIKNQTIIINGVSKTYAMTGWRIGYAVGNSEIINGMIAVASQSTSNPTAVSQ
                     YAAVEALKGEQDTVEEMRNAFEERLNTLYPLVAELPGVSLEKPQGAFYLFPNVKETLR
                     MCKYENVTQWVEDLLEETGVALVTGEGFGAPENVRMSYATDRATLEEAVRRIKQFIES
                     KSQN"
     gene            5873..7171
                     /gene="asnS"
                     /locus_tag="ACG842_001997"
     CDS             5873..7171
                     /gene="asnS"
                     /locus_tag="ACG842_001997"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HFQ0248528.1"
                     /translation="MEQIQIIDSKNHVGETVTIGAWVANKRSSGKITFLQLRDGTAFF
                     QGVVVKSEVSEEVFQTAKNLHQETSVLVTGEIREDSRSKFGYEIGITNVEVVSESHDY
                     PITPKEHGTDFLMDHRHLWLRSSRQHAIMQIRNEIIRATYEFFNQHHFVKIDPPILTG
                     SAPEGTTDLFETNYFDQKAYLSQSGQLYMEAAAMAFGKVFSFGPTFRAEKSKTRRHLI
                     EFWMIEPEMAFTHQEESLEVQEQYVAFLVQNVLDHCEYALDVLGRDKEVLKTYTKLPF
                     PRITYDDAIELLKKNGFDDIEWGEDFGSPHETFIANSFEQPVFILNYPKAIKPFYMKP
                     HPTREDVVICADMIAPEGYGEIIGGSERATDYDFLLEQIRNHGLDEKEYSWYLDLRKY
                     GSVPHSGFGLGLERTVTWIAGIDHVREAIPFPRLLNRIYP"
     gene            7966..8640
                     /locus_tag="ACG842_001998"
     CDS             7966..8640
                     /locus_tag="ACG842_001998"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HFQ0248529.1"
                     /translation="MKKRILFIGDSITDAGRYRAEATDLGKGYPLLIAGKLISQYPTM
                     EFECMNRGIGGDKIADLQVRWQEDCLALQPDYVSILIGINDTWHNTSDQAIFGTEKSA
                     QQFESVYRKLLNELQTAEICNIILMEPFVLPITDDRHGWRKDLDPKIQIVRKLAKEYG
                     ALLVPLDGIFNALGIAYGFSKYTREDGVHPTIGGHEIIAQSWIDAIVAVDFFSGDKNE
                     RKKSEK"
     gene            8637..8978
                     /locus_tag="ACG842_001999"
     CDS             8637..8978
                     /locus_tag="ACG842_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0248530.1"
                     /translation="MMEIRWAVKEDLPQINQLITDHPESFVKAKTAADLNDKNKLLLV
                     AVTKEKRIIGFAQADVQQSEERLDQRPSGKLIQHTLVTKENTEEIQKKFAEYLMLEAK
                     KRRIKSIFAQF"
     gene            9008..9367
                     /locus_tag="ACG842_002000"
     CDS             9008..9367
                     /locus_tag="ACG842_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HFQ0248531.1"
                     /translation="MSCLGNVIWFIFGGLIGGLSWLAAGILWCITIIGIPIGMQCFKI
                     ASLSFAPFGKEVIYHQSGMSLIVNIIWLIVSGLPIALAHVVSGILLCITIIGIPFAKQ
                     SFKLARLALMPFGAEIV"
     gene            9627..9749
                     /locus_tag="ACG842_002001"
     CDS             9627..9749
                     /locus_tag="ACG842_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0248532.1"
                     /translation="MNLDKLPATGFKLSCYPVKIKKASAGWIRAVAMIEEKKKE"
     gene            9891..11975
                     /locus_tag="ACG842_002002"
     CDS             9891..11975
                     /locus_tag="ACG842_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFQ0248533.1"
                     /translation="MKSYRLEGLDCANCAMKIEKGVQKINGVKEATVNFTSGKLTIDA
                     EEDHLATIEQETKKVVKELEPDVKVTEIDKEKVSEHGNEKKRNTLFRILFSLAGIALL
                     LLFDFNEPIRLIGYLLIYLLIGYDVVKKAVMNIVKGKIFDENFLMSVATIGAMLIGEY
                     PEAVAVMLFYQIGEYFQGLAVSHSRKSIRELMAIRPETAHVQTAEGLMTVNPEDVLIG
                     QFVLVKPGERVPLDGTIIEGESLVDTSALTGESVPKSVYVGETVLSGFINKNKPFLVQ
                     VEKSYENSTISKLLELVENASSKKAPAENFITKFARYYTPVVVGLAVLLAVLPPLVVS
                     RAAFSEWIYRALTFLVISCPCALVISVPLSFFGGIGGASKIGVLVKGSNYLELLAQTE
                     TVVFDKTGTLTKGDFSIQTIDPTGIDPKKFMQYVASAEQFSTHPIAQSVLEGYAGPLL
                     PTANIQEFAGEGILAEVDGKQVLVGNHKLMERFEISFPSSQEIGTLLYLAIDQSYSGY
                     LVIADTLKEDAVDALVQLKQAGVKNTVMLTGDSKKIADHIGKQVGVDKIYSELLPEDK
                     VQRLEEILQSNNKKTAFVGDGINDAPVLARADVGIAMGGLGSDAAIEAADVVIMNDQP
                     SKIAEAIHLAKKTLKIVKQNIVFAIGIKILVLSLGAFGFASMGDAVFADVGVTVLAVL
                     NAMRSLHVKNKR"
     gene            12286..13752
                     /locus_tag="ACG842_002003"
     CDS             12286..13752
                     /locus_tag="ACG842_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFQ0248534.1"
                     /translation="MNNKQLRWFTVALIAFNMVWGMGNVVNNYAQQGVSVVTSWLLIL
                     AIYFIPYALIVGQLGSAFKDSKGGVSSWVENTTSNKRLAYYAAWTYWVVHIPYLAQKP
                     QAILIAGGWAVQGNGNIVNTMSVQMVAVISLIIFLAFLYLSTKGLSTLKVIGGLAGTA
                     MFVMSILFILLAVTAPFINPTMEFATPHMNQVKTYIPTFDFSYFTTVSMLVFAVGGAE
                     KISPYVNSTRNPAKEFPKGMIFLAAMVGICAVLGSVAMGMLFASDNIPKDLMANGAYS
                     AFQILGNYYGIGNSLMIIYALTNMIGQISALAFSIDAPLKILLADADPEFVPNWLRKR
                     NRKGTLVNGYLLTGILVGIIILLPIFGIKDMNELVKWLTNLNSVVMPMRYLWVFLAYM
                     MLNKAYKKFNSEYKFVKNPKIGFIFGAWCFAFTAFACILGMVPKVDYTADPKAWWFQL
                     FTNIITPIVLILLGMILPAIARRDKNKEITEPETVVQP"
BASE COUNT         4635 a         2430 c         2974 g         3939 t
ORIGIN      
        1 aaaaagaggt tgtgacgtgg tcacgacctc tttttgacga ttatgataca aaaaacatca
       61 tttatgaaca aaatgtgaat aacgggcgtt cttgagatat gattttaatt gggaaaaact
      121 tatcgataag gaggtcttta tgaaaaaaag actgttatgg tgtttgctgt ttctatccct
      181 aagtttaaca agtcagccag tttttgctga agaaacacag cggtctccta ttacaggcac
      241 acgtctgtac aaaaatgttt atgtacagac aacagatctt gattctgcga ttcctgaaac
      301 agaagaacgg tctttgcagc caagatttgg ttacttttgg tactgtccga cctgtgatta
      361 tgaaagttgc tggcatttct tacattctac agctgcagct tttgctttta ctcatactca
      421 gaaatatgat ggacatcatc ctgttattta tgaggtatag atatgagcaa aaaagaaaaa
      481 ctaatctcgt tgatcgtatt ggtttgcctt gttcttctgg taactgtcat aggctatcag
      541 aaataccatg agaacatcag acctactaaa gaatcttccc cagcctcgac cgttgaaaca
      601 gaaccgataa gtagtcagaa agaataattt ttttagttta aagaagaaca tcagagcaaa
      661 aaaacaccaa ctgaacaata tgtcgtttat tttaaaaaaa gttcggtttt tcactttatg
      721 taatcgctga gaaaactttt tttaataaaa aaagtcgaaa aactctagct tttttgtaaa
      781 cggtatgata tactatagga gtagtttttg ttcggtaatc tgtggtagct tgtttcaaat
      841 aaacatcttc cagatataca ccattcaagt aattgcaaaa tatgtgttga tacacaagga
      901 ggatttttat tatggaaaca ggtacagtaa aatggtttaa ctcagaaaaa ggatttggat
      961 tcatcactgc agaaaacgga aacgatgtat tcgttcattt ctcagctatc caaggcgacg
     1021 gattcaaaac tttagaagaa ggccaagcag taactttcga tgttgaagaa ggccaacgcg
     1081 gacctcaagc aacaaacgtt actaaagctt aattaaccta tactaaaaaa taaacgccca
     1141 gacatttgcg tgtctgggcg tttttgtcta ttttctttaa aaagcgatga gagtattttt
     1201 ctgcactttg tagtataatt tcaaaagaac attagaggca ggagaagagg aagatatttt
     1261 gaaaccaaga acttatgcag tcgttgatat cgaaacgact gggacgaatc caaaagaaga
     1321 tcggatcatc caatttggct gcgtcatgat tgaatcaggt cggatcactt cacgtttttc
     1381 gatcgatatt catccagggc ggaagatttc aaaacaaatc cagcatttaa cagggatcac
     1441 gaatcaacgc gtccaaaaag cgccttattt cgaagatgtc gcccaaacga tttacaatct
     1501 tttggcagac accatatttg tcgcgcataa tatttatttt gattataatt ttttgaacca
     1561 tgaactcatg cgatgcggac ttcctagttt gaagatacca ggcatcgata cagtagaact
     1621 tgcacaaatt ttcttgccga cggaaccaag ctttcgctta gctgatttgt cagaaagcct
     1681 tggatttatt catgagaatc ctcatcaggc agatagtgat gccgaagtga cgggtcaact
     1741 tcttttgctg atcgaagaac ggatgagaaa attgccaatc atcacgttag agcagattgc
     1801 gcgattaagt agacacacag gtatggatac gagtcgtttc atttatcatg tgattgaaga
     1861 aatgaaagaa aaaccagagc cacttgatcc ctcattagaa atcgtcgatg ggctagcttt
     1921 gcagaaaaaa gaagtggaac tattcacatc cgtccattat ggcgagcgga catatccaag
     1981 aaaaaaacaa gcaaaagaaa aaatgtttgg taaaacactg atgtatcgaa aagaacagaa
     2041 cagactgatg aatgcggtct acgaccattt cacaaaagat gaatcaaaag atttgatgat
     2101 cgaagcagct acaggaatgg gtaaaacaat cggttatctg ctgccattga gttatttggc
     2161 tacacctgaa aaacccgctg tcatcagtac cgtttcgcta gttttgcagc agcagatcat
     2221 agaaaaagac attccattgc tgaatcaatt gctagatcag cccatacaac ctgtaattat
     2281 caaaagctat cgccactata ttgatttaca gcgttttaaa ggcacacttg tggaaccgcc
     2341 agaacaaaaa caatatgctt tgtatcaaat ggcggtctta gtttggctga cgcaaacgaa
     2401 gaccggtgat ttggatgaat tacatttaac aaatttgaac cattcattct ttgcagatat
     2461 cgcgcacaga ggtattggat ttttagcaag aaatcagtca ttttacgaac aagactttgt
     2521 tcgtttcttg caaaagaaaa tcaggcaaag taattttttg attgtcaatc atgcgttttt
     2581 aatacaagaa acacagcgaa aagaaccatt gcttccagct agtccatttt tgttgatcga
     2641 tgaagtacat catttgccgg aaatcgctga aaaagtaaat gacaaacgat taagtattaa
     2701 ctatttccat aaacaaatcc agcaattaga agaaccaaat cagctgtttg accgaataca
     2761 aagctattta ccgaaagaac atgaagcctt acgctggtta tcgatttatc gagaagaact
     2821 ttctgcgcta tgcgaagcac agatttgtat ggcagaagga ttaatgagtt tagtgagcgg
     2881 caagcaacct aacggacagt atccagaaga agtcatgatg acaaaagaga tgttggatca
     2941 gctgccactg gaatctggga cagccattga tcacctgcag cttttttatc aggagatcca
     3001 gcagctacag tcacaaatgc gtctgacttt tgagattatc caagaaagct ggacaaacca
     3061 gatgcggatc gatttagcaa gtttatatag cttatttgat caattagaca agcagtactt
     3121 gctgatggat caatggctgg aagactggcg agatcatttg atccattggt tcgttcctgg
     3181 gaacacagcg cagcagatga tttttcatct ccatgatttt caagcggcta tgttgccgtc
     3241 gactgtttgg tatcctcgat atgatcgtat tctttatatt ggaggaacgc tgaagatcgg
     3301 ccctaatcga cactatctct ctaagcggct tggcattgaa gcagcacctc ttaaagtgat
     3361 tgcctctcct tatgattatg agaaacaggc aaggatctat gtgccaaaag aggcacctgc
     3421 cgtccatcaa gtcagcaatc aagcatatat cgattattta gagacagcac ttacaggtct
     3481 gatcgaacaa gaagatcggc caatacttgt gttattcact tctcatgatg ttttgcagaa
     3541 ggtctatcag aaaatgcatc tgtctatgct agaaaaagga cgagaaatac tagctcaagg
     3601 gtttggcgga agccgggaga agcttttgaa acgcttcatt ttgtcagaaa aaagcgtatt
     3661 gtttggcgct gacagctttt gggaaggtgt agatttgcct ggggatgctt tgcagatcgt
     3721 tgtagtcaca cgtttaccct tcgataatcc gcttcgcccg atggtcaaag ctaggaacgc
     3781 ttatttagaa tcagagggga tcaatccttt ttaccaggaa tccgtaccta aagcagcttt
     3841 gaagttacgc caagcattag gtcgcttgat aaggacggag acagatcgtg gcgtattgct
     3901 tattttggat cgaagactag tcactgcaaa atatggtgca cggatcatcc atgcattacc
     3961 gaaaaaagta ccgatcaaag agctagctat ggaggaaatc tatcaggaca tccatgaatt
     4021 cttaaagaaa aatgaagaag gaagttagag gagctgtctt ttttcataat taaaatctgc
     4081 tataatgaaa aggaatgaag ataaaggagc gacgaccctt gaagaaaaaa cagacaaagg
     4141 aaaaaacgat taatcgcgtg ttacttggct tgatcgtatt tttattagcg gtcatcgtga
     4201 tcagctcgat tttttacttg cgttccagtc gtccggcggt gcaggcgaag aaagaagcag
     4261 tagaaatcgc aaagaaatat gcaaatcttg aggaagccga tcatttttat tggtttactc
     4321 gaaagaagac atacttttca ctgacaggta caaatgataa aggaaaagaa atcgccgtta
     4381 tcattcctaa atctggtgaa aaagtaaaag tattggatca aacaaaggga ctgactgaaa
     4441 acgaagcaaa acagacaatt gctgcggccc atcctgaaat ccaaatcgaa aaagctgctt
     4501 tagggatgta tgatgatcag cctgtgtggg aagtagtagg aaaagaaaca aatgggaaac
     4561 tgaattatta cctgattggc tttgaatctg gagatgaagt caagacaatc aagggaattt
     4621 aaaaggtaaa ggaatgaatg ggatggagtt atcaaaaaga acaaaacgat tagaaccgtc
     4681 tgtcactttg gcagctgcgg caaaggccaa ggcattaaaa gcaaaaggca gagatgtgct
     4741 gagtttgact gtgggagaac cggattttgc gacaccagaa aacatccaag aagcagcaat
     4801 cgaagcaatc cgcaatggga aagctagcta ttatacacca acagcaggaa tcccagagct
     4861 tcgacaggca atcgttgatt atctgaagaa aaactatgga cttgtctatg aaccttcaca
     4921 aacagtcgta acagacggag cgaaatttgc tttgtatacc cttttccaaa cgatccttga
     4981 cccgcaagat gaagtcatca ttcctgtgcc ttactgggtc agttacggtg agcaagtaaa
     5041 actggcagaa ggacttcctg tctttgtgaa aggggaagaa tccaatagct ggaaagttac
     5101 tgttgaacag ctagaacaag cacgttcaga aaaaacaaaa gccgtaatca tcaattctcc
     5161 atcgaatccg actggaatga tttatagcaa gaatgaactt caggcaatcg gtgaatgggc
     5221 agtaaaaaat gatattttga tagttgctga tgatatttat ggtcgtttag tttataatgg
     5281 caatgagttt actccaattg caacgatttc agaagcaatc aaaaatcaga ccatcatcat
     5341 caatggcgtg tctaaaacat atgcgatgac aggctggcgc attggttatg cggtggggaa
     5401 ttctgagatc atcaatggaa tgattgctgt tgcttcccag tcaaccagta atccgacagc
     5461 agtcagtcaa tatgcagctg ttgaagcatt gaaaggtgag caagatacag tagaagaaat
     5521 gcggaacgcc tttgaagaac gcctgaatac gttatatccg cttgtggcag aactgccagg
     5581 tgtttctctt gaaaagccgc aaggagcttt ctatcttttt cctaatgtaa aagaaacgct
     5641 acgcatgtgt aaatatgaaa atgtcacaca atgggtagaa gatcttttag aagaaacagg
     5701 ggtagcgctt gtgactggcg aaggctttgg ggcaccagaa aatgtccgca tgagttatgc
     5761 tactgatcgc gctacgttag aagaagcggt acgcagaatc aagcaattta ttgagagtaa
     5821 aagtcaaaat taatcctgaa gaaattcaag gaatataaaa ggagagtcat acgtggaaca
     5881 aattcaaatc atcgattcga aaaaccatgt gggagaaaca gtaacaatcg gcgcttgggt
     5941 cgcaaataag cgatcaagcg gaaaaatcac atttttacaa ttaagagacg gaacagcctt
     6001 tttccaaggt gtagttgtaa aaagcgaagt gtcggaagaa gttttccaaa cagctaaaaa
     6061 tctacaccaa gaaacatccg tgttagtaac tggagaaatc cgtgaagaca gtcgttcaaa
     6121 atttggttat gaaatcggaa ttacaaatgt ggaagtggta agcgagagcc acgattatcc
     6181 gatcacacca aaagaacacg gaacagactt cttgatggat catcgtcatt tatggctgcg
     6241 ttcttcaaga caacatgcga ttatgcagat cagaaatgaa atcattcgtg caacgtatga
     6301 attctttaat caacatcatt ttgtcaaaat tgatccgccg atcttgactg gttctgcacc
     6361 agaaggcact acggatctat tcgaaacaaa ttactttgat caaaaagctt atttatctca
     6421 aagtggccaa ttatacatgg aagctgctgc tatggctttc ggaaaagtct tttcattcgg
     6481 tccgactttc cgtgctgaaa aatcaaaaac acgacgtcat ttgatcgaat tctggatgat
     6541 cgaaccagaa atggctttca ctcatcaaga agaaagttta gaagtacagg aacaatatgt
     6601 agcattctta gtacaaaatg ttttagatca ttgcgaatac gcattagatg tactaggacg
     6661 agacaaagaa gtcttaaaaa cttatacaaa attgccattc cctcgtatta cttacgatga
     6721 tgcaatcgaa ttactgaaga aaaatggatt tgatgacatc gaatggggcg aagactttgg
     6781 ttcacctcat gaaacgttca ttgcgaattc attcgaacag cctgtcttta tcctaaacta
     6841 tccaaaagcc atcaagccat tctacatgaa accacatcca acaagagaag acgtggtaat
     6901 ttgtgcagat atgatcgcac cagaaggtta cggtgaaatt attggcggaa gcgaacgggc
     6961 aacggactat gatttcttgc tagaacaaat ccgtaatcac ggtttggatg aaaaagaata
     7021 ttcatggtat cttgatctac gtaaatacgg ttctgttccg cactctggtt ttggtctagg
     7081 tcttgaacga acagtcactt ggattgctgg tatcgaccat gtacgtgaag ctattccatt
     7141 cccacgtttg ttgaaccgaa tttatccata agaggttgtg cgcctgccgt tcagctccga
     7201 gtcacaagga gtaatttcaa aaaacagttt ttcatgtttg atgactattg tgacttgtgg
     7261 ccggagagca acgtttattt aaaaaaaaga actgcgaact ctcgcagttc ttttttttaa
     7321 atacgggata atggaagttt ccagcagaca agagaagaaa gcaaaagaaa ttgctatttg
     7381 attcacaagg ttctataacg aaatcgcaat caatcaaaca tttacaaaga aggtaattca
     7441 aatggaaatc tgatttgatg atgcttctgc tgttgaaata aagcagcatc tagcacctga
     7501 aaacaaacta atagtaacat tcgaagatgg agtaggtccg tattttcagc atgcaataat
     7561 ccatatgtaa gttcaattca cccttgaaat catcggacca gataaagtta tgaccaatct
     7621 atcccaagca atctagaaga gatattagta aagggacatt tactagaagt cttagatgag
     7681 cctataaata tatgtttcga tggcgtcatc agtggtctga ttgacggaag cttgggtgtg
     7741 atcgatctct ctggaccaaa caacgaagga atcaagggga atccggcacg ttaagaaaaa
     7801 aatagcataa aaaactagac acttgatgag cgttcattca attgtttgaa ggaactaggc
     7861 tggtgtcatt ttttatacat atattttctc tttcgtcttg atagggctta tgggataata
     7921 taaggaagaa aatcatcgtt ttattcaaaa aggagttttt gacaaatgaa aaaacgtata
     7981 ttatttatcg gtgatagcat tacagacgca ggaagatacc gagcagaagc aactgatcta
     8041 ggtaaaggtt atccactttt gattgccgga aaactaatct cccaatatcc aacgatggaa
     8101 tttgaatgca tgaaccgtgg gataggagga gacaaaattg cagatctgca agtacgctgg
     8161 caagaagatt gtttagcttt acaacctgac tatgtatcga ttttgatcgg aatcaatgat
     8221 acctggcata atactagcga tcaggctata ttcggaactg agaaatctgc tcagcaattt
     8281 gaatccgttt atcgaaaatt actaaatgaa cttcaaacag cggaaatctg caacatcatc
     8341 ttgatggagc cgtttgtact tcctataacc gacgaccgtc atggctggcg taaagactta
     8401 gaccctaaaa tccaaattgt tcgtaaactt gctaaggaat atggcgcatt gctggttcct
     8461 ttagatggta tattcaatgc tttagggatt gcttatggtt tttcgaaata tacaagagaa
     8521 gatggggtcc acccaacgat tggcggacat gagatcattg cacaaagctg gatagatgca
     8581 attgtcgcag tcgatttttt tagtggagat aaaaatgagc gaaagaaaag tgaaaaatga
     8641 tggaaataag gtgggcagta aaagaagatt tacctcaaat caatcagctg attactgatc
     8701 atccagaatc attcgtaaaa gcaaaaacgg cagctgacct aaatgataag aataaactat
     8761 tgctcgttgc tgtaacaaaa gaaaaacgga ttatcggatt tgcgcaagcg gacgtgcaac
     8821 agtcagaaga acgattggat cagcgaccat caggaaaact aatccaacac acattagtaa
     8881 caaaagaaaa tacggaagaa atccaaaaaa aatttgcaga atacttgatg ctggaagcta
     8941 aaaaaagaag aataaaatca atttttgccc agttttaatc aaaaacagta atagaaggag
     9001 aaatacaatg agttgtttag gaaatgtcat ctggtttata ttcggcggac taatcggtgg
     9061 tcttagctgg ttagcagcgg ggatactttg gtgtatcacg atcataggta tcccaatcgg
     9121 tatgcaatgt tttaagatcg cctccctttc ttttgcgcct tttggcaaag aagtaattta
     9181 tcaccagtct ggcatgtcat tgatcgttaa tatcatctgg ctgatcgttt caggcctacc
     9241 aattgctctc gcacatgtcg tcagcggcat ccttctatgt atcacgatca tagggatacc
     9301 ttttgccaag caatctttca aacttgctcg attggcgttg atgccattcg gtgcagaaat
     9361 tgtataaaag agaataataa aggttagata aagcatagag ccaggtggga tacatgtatt
     9421 tcacctgact ctatgcgttt tcctgtataa gttcttctat gtgactttta cgtcttttat
     9481 ttatatgggg ttactgttta tggaaagttg aaataatttt cattaaagaa ctctttctat
     9541 atttttgaaa gcgtagtgga gcttcgtata tctgggaagg acattttgca gggattgaaa
     9601 aaagatattg tcctatcgaa aaattaatga atttagacaa attgccagca acaggcttca
     9661 aacttagttg ctatcctgtc aagatcaaga aggcaagtgc gggttggata agagcagtgg
     9721 caatgatcga agagaaaaag aaagaataag ccaaaacatc tgtatggaag aaattttccg
     9781 atgcagatgt ttttcatttt ttctttcgga taccttgaca tatgaataac cattcattta
     9841 taattagtta tgaagagata ttcatatgtt gaggcgagga ggtcattaaa atgaaaagtt
     9901 acagattaga aggtttagat tgtgcgaact gtgcaatgaa aatcgaaaaa ggagttcaga
     9961 aaatcaatgg agtcaaggaa gcaacggtaa acttcacttc tggaaaacta acgattgacg
    10021 cagaggaaga tcatttggct acgattgaac aagaaacaaa aaaagtagtg aaggaattag
    10081 aaccagatgt caaagtaaca gaaatagata aggaaaaagt atctgaacat ggaaatgaaa
    10141 aaaagagaaa tacgctgttc cggattttat tttcccttgc aggtatcgct ttattgctat
    10201 tatttgattt taatgaacca attcgcttga tagggtattt attgatttat ctgctgatag
    10261 gatatgatgt agtgaagaag gcagtgatga atatcgtcaa aggaaaaatc tttgatgaga
    10321 attttcttat gtctgttgct acgattggcg ctatgttgat cggtgaatat ccagaagccg
    10381 tagcagtgat gctattttat caaatcggcg agtatttcca aggattggca gtcagtcatt
    10441 caagaaaatc gattcgcgaa ttgatggcga tccgtccaga aacagctcac gtgcagacgg
    10501 cggaaggatt gatgaccgtg aaccctgaag acgtgctgat cggtcagttt gtacttgtga
    10561 agcctggtga acgtgtaccg ttagatggaa cgatcatcga aggagaatca ttagtcgata
    10621 cgtcagcact tacaggagaa tcagtcccta aatctgtata tgttggtgaa acagtattaa
    10681 gcggatttat caataaaaat aaaccatttt tagttcaagt agaaaaatct tatgaaaatt
    10741 ctactatcag caaattatta gaattagtag aaaatgccag cagtaaaaaa gcaccagctg
    10801 aaaatttcat tactaaattc gcgcgctatt acacaccagt cgttgtagga ttggccgttt
    10861 tattagctgt tttacctcca cttgttgttt ctagagcggc gttttctgaa tggatctatc
    10921 gagcattgac atttttagtg atctcatgtc catgtgcatt agtgatttcc gtgccactta
    10981 gtttctttgg cggtatcgga ggagcaagta aaatcggtgt attagtaaaa ggttcaaatt
    11041 acttggagtt gcttgcacaa actgagacag tggtattcga taaaactggc acattaacga
    11101 aaggtgactt ttcgatccag acgatcgatc caacaggaat tgatccaaag aaatttatgc
    11161 aatatgttgc aagtgcagaa cagttttcta cacatccgat tgctcaatct gttttagaag
    11221 gctacgcagg tccgctttta ccaacagcta atatccagga atttgcaggt gaagggatct
    11281 tagctgaagt tgatggcaaa caagtattag taggtaatca caaattgatg gaaagatttg
    11341 aaatctcttt tccttcaagt caagaaatcg gtacattgct ttatctagcg atcgatcaaa
    11401 gttacagtgg ttatcttgtt attgcagata ccttgaaaga agatgccgta gacgcattgg
    11461 ttcaattaaa acaggctggt gtgaaaaaca cagtcatgtt gacgggcgac tcgaaaaaaa
    11521 ttgctgatca tatcgggaag caagtaggcg tggacaagat atatagcgaa ttgcttccag
    11581 aagataaagt gcagcggtta gaggaaatct tacaaagtaa caataaaaag accgcatttg
    11641 tcggagatgg aatcaacgat gcaccggtgc tagcaagagc agatgtagga atcgcaatgg
    11701 gtggattggg aagcgatgct gcaattgaag cagcagatgt agtcatcatg aatgatcaac
    11761 catcgaagat tgcagaagca atccatttag caaagaaaac attgaaaatc gtcaaacaga
    11821 acattgtctt tgctattggg ataaaaattt tggtactttc cttaggtgct tttggttttg
    11881 catcaatggg agatgcagta tttgcagatg ttggggtgac agtattagct gttttgaatg
    11941 ccatgcgaag cttgcatgta aaaaataaac gttgatcaat gaagaggacg aatgaacgtc
    12001 ctcttttttt tatatccttt tcttaatgaa aaatgtaaag aaaatagata ggattataaa
    12061 ataactaatg tttttaattt tgcagaaaat tcaaaatagc ttgaaattca ataaggtcaa
    12121 tgaaataaaa gaaaataact tatttttttg atggaaaaaa tatgaaaaaa cgatgtgtat
    12181 taaataaaag aatacacaca taaaataaca ctgtcttgta agtttgttaa aaattgaata
    12241 tgattacatc tggataacaa atttttatca taaaggagag aacgaatgaa caacaaacaa
    12301 ttacgctggt ttactgttgc gttaattgca tttaacatgg tttggggcat ggggaatgta
    12361 gttaataact atgctcagca aggagtatca gttgtcacct cttggctatt gattttggcc
    12421 atttatttta ttccatacgc attgatcgtt ggacaattag gatcagcctt caaggatagt
    12481 aaaggaggcg tcagttcatg ggtcgaaaat acgacatcta ataaacgact ggcatactat
    12541 gccgcttgga cttactgggt cgtccatatt ccttatttgg cacaaaagcc tcaagcaatt
    12601 ctgattgctg gtggttgggc agtccaagga aatggaaata tcgtaaatac gatgtcagta
    12661 caaatggtag cagttatttc acttattata tttcttgcct ttttatattt atcaacaaaa
    12721 ggactttcta ctttgaaagt gattggcgga ctggccggaa cagcgatgtt cgtcatgtcg
    12781 atcttattta ttcttttagc tgtaacggca ccgttcatca atccaacgat ggaatttgcc
    12841 acaccacata tgaatcaagt gaaaacatat attccaacat ttgatttcag ttatttcaca
    12901 accgtttcga tgctagtatt tgcagttgga ggggcggaaa aaatatcccc ttatgtcaac
    12961 tctacaagaa atcctgcaaa ggaatttcct aaagggatga tcttcttagc agcaatggta
    13021 gggatttgtg ctgtattagg ctcagtcgca atgggtatgc tgtttgctag cgataatatc
    13081 cctaaagact tgatggcaaa tggggcatac agtgcttttc aaattttagg taattactat
    13141 ggtatcggta actcattgat gatcatttac gcattgacaa acatgattgg acaaatctcc
    13201 gcattagcct tttcaattga tgcaccacta aaaatcttgt tagcagatgc ggatccagaa
    13261 tttgtaccaa actggctacg taaacgtaat cgtaaaggta cattggtgaa tggttatctt
    13321 ctgacaggta tactagtagg aatcattatt ttgttgccga tctttggtat caaagatatg
    13381 aatgaattag taaaatggtt gacgaacttg aactcagttg tcatgccaat gcgttaccta
    13441 tgggtgttct tggcatatat gatgttgaat aaagcttata agaaattcaa ttctgaatat
    13501 aaatttgtta aaaatccaaa aatcggtttt atctttggtg cttggtgctt tgccttcact
    13561 gcgtttgcat gtatcctagg aatggtgcca aaagtagatt atactgctga tcctaaagca
    13621 tggtggttcc aattattcac gaatatcatc acaccaattg tcttgatctt attaggtatg
    13681 atcttgccag caattgctcg tcgagacaaa aacaaagaaa ttactgaacc agaaacagtg
    13741 gttcagcctt aaaatagaaa tacttgttac aagaggttgt gacagaagct acttctggaa
    13801 caccgtgaat cagaggttgt gaaaaagctg tcctgcatca agtaataaga acagccaaac
    13861 aaaaatagct cctcatattt tgccgttttt tgagctaatg ctgaaggtca gctgattgaa
    13921 caccatttat tcgaaacaag ggactttgac acgacttttg tcacagtccc ttgttttt
//
LOCUS       DAZKLM010000071        13909 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3764
            SAMEA115706913-rid22582563.denovo.073, whole genome shotgun
            sequence.
ACCESSION   DAZKLM010000071 DAZKLM010000000
VERSION     DAZKLM010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706913
            Sequence Read Archive: ERR13196647
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13909)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13909)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287823.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287823.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,004
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,849
            CDSs (with protein)               :: 2,849
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 41 of 96
            Pseudo Genes (incomplete)         :: 52 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 15 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13909
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706913-rid22582563.denovo.073"
                     /isolate="VRE3764"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..226)
                     /locus_tag="ACG842_002004"
     CDS             complement(<1..226)
                     /locus_tag="ACG842_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HFQ0248535.1"
                     /translation="MKKRKGFNHFFMNLWRYKALVLMAVPGMLWMIFFFYIPVMANVV
                     AFKNFHISADGFLASLRESPWVGWENFKFLF"
     gene            complement(705..1424)
                     /locus_tag="ACG842_002005"
     CDS             complement(705..1424)
                     /locus_tag="ACG842_002005"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HFQ0248536.1"
                     /translation="MRLDKFLADMGLGSRKEVKGLIKKGTIEVNGQVVKSDKAQVNEF
                     EDAVTYLGEIITYQKDFYYILHKPAGVISATQDNHDQTVIDLLEDQDYREDLFPVGRL
                     DKDTEGLLILTNDGVFAHQLLSPKKHVEKEYLAEVSGIMTEEDIRLFAEGLIIDGDEQ
                     TLPAKLQIDQVDQETETSIIRLILHEGKFHQVKRMVKAVGKEVLYLKRIRMGKFWLPK
                     DLNKGQYRPMTKEELEQVREK"
     gene            complement(1473..3119)
                     /locus_tag="ACG842_002006"
     CDS             complement(1473..3119)
                     /locus_tag="ACG842_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis C-terminal
                     domain-containing protein"
                     /protein_id="HFQ0248537.1"
                     /translation="MDNQRKPALDQLTPQEKMARGSAWMTASNIISRLLGAVYIIPWY
                     AWMGENAKAANGLFNMGYNIYALFLLISTAGIPAAIAKQTARYNSLNEYGTSRRLFIR
                     ALQMMGGLGLLFALFMYIASPWLAHASGGGEELIPTMRSLSIAVLVFPCMSVIRGYLQ
                     GNQEMMPYALSQIVEQVARIFYMLLATFIIMKVMDGDYVTAVTQSTFAAFIGMLASIL
                     VLLYFLKKHQAYTSAFIHYSENKINIATKELLLDTVKEAIPFIIVGSGVTIFKLVDQF
                     TFMKIMSASTEYSNAQLLDLFSIFSANPDKLTMVVIALATSIASTGLPLITEAVTLKD
                     RVGLAKLTSGNLQLFSFFMFPATFGVMLLAYPLNTLFYTPDSLGSNVLIQASFVGLFL
                     GLYMLVSNMLQGMFENKAAINYLVIGFLVKLLLQYPAIRLFEVYGPLLATMIGFAVSC
                     TLILKRIHEVARFKPSFVWRRTLLIFILTLIMLLASWITKMIFGAFLNEDSKFQSLLL
                     ILVVAGIGALVYGYLALKIRLAERLLGPGIARLRHKLKIK"
     gene            3298..3888
                     /locus_tag="ACG842_002007"
     CDS             3298..3888
                     /locus_tag="ACG842_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFQ0248538.1"
                     /translation="MTQEIKKLPVIFLGLFFITVGLNWFLLPHDIASAGVGAIGHLVE
                     TAFSIQRSVTVWSINLMMLLLAAVLLGKSVFFKTVAGSLLFPVVLEIVPKVEMISSHF
                     FSLISGSLLFSLGVYTLYTVGASNGGITIPPIIFHKFFRLPMPQGLLLTNSLIVFLNY
                     YVFGLLETLFVLLSITVSSLFMKILIRLSPVSRKFE"
     gene            3980..5485
                     /locus_tag="ACG842_002008"
     CDS             3980..5485
                     /locus_tag="ACG842_002008"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HFQ0248539.1"
                     /translation="MSFSLEDIRHLLLKENLLKEFVSTRGWHLDVPEKAEFLQLSYDS
                     RKADASTLFFCKGMNFKEEYLDSAIEQGIQYYVSEQPYENSAVGIIVTDIRKAMALLA
                     MAFYDYPQNKLKVIGFTGTKGKTTAAYFTKAILDHTTNKKTALLSTMNTTLDGKTYFK
                     SHLTTPESLDLYRMMSEAVENGMTHLVMEVSSQAYKTQRVYGLTLDVGIFLNISPDHI
                     SPIEHPTFDDYFYCKRQLILNSKTVVLNHESDYFDLLRETADLFQIPTITYGRSEESN
                     YQVIRSDKGTHGFTLSSHEDRLAICDTAYDILLAGGFNQENAASAIIAAALTGASSQD
                     AQDGLKEARVPGRMDQLIQSNGAHVYVDYAHNYLSLKTLLKFAKNEHPDGRVIVVLGS
                     PGNKAISRRHDFGKVLSETADIAFLTADDPAFEDPKKIAEEINEAITNPDLIVHYEMD
                     RPEAIRRALAESAPQDSVVIAGKGVDPYQKINGVDEPYEGDYAIVKRLIEE"
     gene            complement(5569..6921)
                     /gene="celB"
                     /locus_tag="ACG842_002009"
     CDS             complement(5569..6921)
                     /gene="celB"
                     /locus_tag="ACG842_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HFQ0248540.1"
                     /translation="MFDFLQKYLMGPMGKIAQLKIVRAVMAAGMASIPFTIVGSMFLV
                     LNILPLPFPFLEGFFNATFFKVSDLYMIVNTMTMGILSIYFAIVFAYELTSIERDEQK
                     LNVNPLTGALLSVFAFFMCIPELIMTDGKISLIASINETETVVNGIRMGSFVERLGTS
                     GIFTAIIMSIIAVELYCMCVKRNWVIKMPDVVPPGVSRSFTALIPTFVIAFVVMIING
                     LLVALGTDIFKMIAIPFGFVTQLTNTWLGVMVIYFLIHALWIVGIHGANIITSFLTPI
                     VLANMAANAQGANYPLAGEFNNSYVTVGGSGATLGLIIFIAFLAKSDQLKVLGKASLV
                     PGLFNINEPIIFGMPIVYNPYLAVPFFLAPMASATLAYFAIRLEIVKPMLAQMPWPSP
                     VGIGAFVGSGGDWKAAVLAVICAIIAFIIWFPFIKFYDNKLLNEEREKAAELASEKLK
                     "
     gene            complement(6921..7439)
                     /locus_tag="ACG842_002010"
     CDS             complement(6921..7439)
                     /locus_tag="ACG842_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0248541.1"
                     /translation="MQDTVKRKIELEKKQLSLKNMYFNRYLIVRYLTAFFFFMNMQWM
                     ILLLSAKSLGSSLPMILILAILPAVGEQVKLYRKHQTNVPWTKWYFLFQSVFNLLVIP
                     VLFTSGFTFLYPFMANNNGGQLFVFILVVSGIFASAFIQYRLKKISLNQDQQYIRIKQ
                     YEKALYLGKENN"
     gene            complement(7455..7781)
                     /locus_tag="ACG842_002011"
     CDS             complement(7455..7781)
                     /locus_tag="ACG842_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIA"
                     /protein_id="HFQ0248542.1"
                     /translation="MAEKINSETLQVTSFDIIFHSGNARTLIHEAFRSMRKEMFEEVE
                     EKLRQANEEILHAHQSQTQLLKEYASGTKIEMEIIMVHAQDHLMTTMTLLEVAQEMNY
                     LYQKNK"
     gene            complement(7794..9773)
                     /locus_tag="ACG842_002012"
     CDS             complement(7794..9773)
                     /locus_tag="ACG842_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFQ0248543.1"
                     /translation="MKQKEKLLLRYLIDHKKEYVTSQRLASELLLSDRTIRNYLQRIK
                     ELVEKNGGKIIAKPGYGYQLHILQRLTFDLFLSRQEIVPGYAREPQGFYESEDRQKYI
                     LNQLLLEDAILFMDDLAEELYISRSSLTKDMQEIKERLIPYSLKIVSKHGQGTWIEGE
                     ERNRRHFIMDTFFGNHYGNSLKEYLGQSRFFPDISFEELVIIILDETREAKLKVSDFI
                     IQNLTLHLALGIKRLREGFEIKELGIEQEVSKRVEYQVAQRIVQRIEVIAGVSFPEEE
                     IAYLTLHLMAKSNHKENQNDQELSKELAKVLKRLSQEFHFSLVEDLQLFNGLLDHLSP
                     MMIRLSRGIRIENPLTEEIKKENPKVFDAVKRHFSNMPALKNYTINEDEWAYLALHLM
                     AALEKERAAHKLHALIICATGYGSAQLLKNRVVSEFGKNITVVSVKGYYEINETTLKG
                     IDLIISSIDLSTMFFKIPVLHVSIFLNEDDVRKIRKVVGESIPYHSSDRPIPAFSLQQ
                     KKQIYTEQLSEKFFKTYTYEPKKEEILHDLLQKLSINEEEGYIREMRKQIQQRENMGQ
                     IIFSDTVAVPHPAVPVGIETKVAVALIPSGMQWDQYQEIHFVFLVSPSYVENEGITVV
                     TKAIVRLVDQLKVQQEILAEPIFENFSSKFIKLIE"
     gene            complement(9794..10108)
                     /locus_tag="ACG842_002013"
     CDS             complement(9794..10108)
                     /locus_tag="ACG842_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIB"
                     /protein_id="HFQ0248544.1"
                     /translation="MKKALIICAAGMSSSMMASKTTDFFKQKREEVLVDAVSATEGDN
                     MIKNSDFDLFLISPQTTMFLDKFKKLGESVGKPVVSIPFQAYVPIPTGIQKLAEVIEE
                     NI"
     gene            10276..10569
                     /locus_tag="ACG842_002014"
     CDS             10276..10569
                     /locus_tag="ACG842_002014"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase family
                     protein"
                     /protein_id="HFQ0248545.1"
                     /translation="MSITVNILYTSTNGQARKFAEEMVERNIVKAIRAEEGNEKYDYY
                     FPLEDPESLLLIDRWKDEEAIEKHHKSEMMAQINELRKKYKLKMKVERFTDLP"
     gene            complement(10647..11759)
                     /locus_tag="ACG842_002015"
     CDS             complement(10647..11759)
                     /locus_tag="ACG842_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFQ0248546.1"
                     /translation="MTVKNKSKKKGRQQVDFYQSLQLDPFILKQKIREAASKKEKCMY
                     ICSLFLRSLFIVLFAICFIIIITTLFESKHKPYAVVLFCMLMSIRFVDFGYKISHSII
                     GLAIVMFSLLVAPYVQLIKWSVMGMLIHFILLSSILMATASDPKMGNASLYGFSYLFI
                     VYSLPKDSLNKNFFTQTSSLLFLFFCWFSVLLYRKHREKNKDKSLFKEIFLKGMYSQE
                     KIWMLIYAFGISLLIVAGEYVPFQRLMWAGFAFSSIVSSYGLMSIGFKERAVDRIIGS
                     LIGCVLFIGISQFIPFNWVGILGGLALGVCSTYRYKTVFNSFGALAITASLFGVPGAV
                     TIRIFENILGVCLGIMYIGVTEILIRKIREKHGLNH"
     gene            11912..12760
                     /locus_tag="ACG842_002016"
                     /pseudo
     CDS             11912..12760
                     /locus_tag="ACG842_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297185.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(13113..>13909)
                     /locus_tag="ACG842_002017"
     CDS             complement(13113..>13909)
                     /locus_tag="ACG842_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HFQ0248547.1"
                     /translation="DATPIEFEIERSQTAVVELTKANRLTPGGVVLTKIDDQSGEVLQ
                     GAVFELQDANGTVLQSGLTTGADGKLAIDGLAPGVYQLVETQAPTGYELDATPIEFEI
                     ERSQTAVVELTKENRLTPGGVVLTKIDDQSGEVLQGAVFELQDANGTVLQSGLTTGAD
                     GKLAIDGLAPGAYQLVETQAPTGYELDATPVTFKIEKEQEVAIFLTKENRKVADSSDI
                     RGNNPTSSGNKHLPKTGETIISQFILSILGVLLVFISIKFRKKRNI"
BASE COUNT         4348 a         2841 c         2377 g         4343 t
ORIGIN      
        1 aaaataaaaa tttgaaattt tcccaaccca cccaaggact ttccctcaag ctagccaaga
       61 acccatctgc tgaaatatga aaatttttga atgctacgac attcgccatt acaggtatgt
      121 aaaagaaaaa aatcatccaa agcatacccg gtacagccat caggacaagt gctttataac
      181 gccatagatt cataaaaaaa tgattgaacc cctttcgttt tttcatctaa aaaccccttt
      241 cgattcattc gcaacgtaat tatagcgctt tcaataatga gaataaataa acaaagtata
      301 gggaaaatga aacaaagtat aggagaggtt gtgaaatcag cgttttggcc tgagtattag
      361 agaaaaaaac gggaaaatag ttcctcatat tttgccgttt tttgagctaa taccgagggt
      421 cagctgattg aacaccgttt atcagaggtt gtgaattcag cgttttggcc tgagtattag
      481 ggagatcgtg tccttactat ctaactctga gtcacaagga acaatgggga ctgtaacaaa
      541 agtcttgtca cagtccctta ttccgaacaa acggtgttcc agaagtagct tctacggaaa
      601 taagccgaaa tttcacaaaa aagccatccg aaagtcagac ggacaagtct gcttttcaga
      661 tggcgctaca actactctgc ttattctttt agctatccat tcaattattt ttctctcact
      721 tgttctagct cttctttggt catgggtcga tattgccctt tattcaaatc tttaggcagc
      781 caaaattttc ccatacggat tctttttaaa tataagactt cttttcctac tgctttgacc
      841 attcgcttaa cttggtgaaa ctttccttca tgcaaaatca gacggataat tgaagtttct
      901 gtctcttgat ctacttgatc aatttggagt tttgccggca atgtctgctc atcgccatca
      961 ataatcagtc cttctgcaaa taaacggata tcttcttctg tcattatacc gctaacctca
     1021 gctagatatt ctttctccac atgtttttta ggtgatagta actgatgggc aaacaccccg
     1081 tcattcgtta aaatcaagag gccctctgtg tctttatcta atcggccgac tggaaaaaga
     1141 tcttcccgat aatcctggtc ttccaataaa tcgattacgg tttgatcatg attatcctga
     1201 gtagcagaaa tgactcctgc aggtttatgc aaaatgtagt aaaaatcttt ttgatacgta
     1261 atgatttctc ctaaataagt aaccgcatct tcgaattcat ttacttgcgc tttatcgctt
     1321 ttcaccactt gtccattgac ttcgatcgtt ccttttttga ttaatccttt gacttctttg
     1381 cggctgccaa gtcccatatc tgctaaaaac ttatctaaac gcatgcgttt cccccatttc
     1441 aaaagagcat caatctagct cttctgattc acttatttta tttttaattt atgtcgtaaa
     1501 cgcgctattc ctggtcctag tagtctttct gccaagcgga ttttcaaagc taaataacca
     1561 tacactaaag cccctatacc tgctactaca agaatcaaca atagagactg gaatttgcta
     1621 tcttcgttta aaaaagcacc aaaaatcatc tttgtgatcc acgaagccaa aagcataatc
     1681 aaggtaagaa taaagatcag caatgtcctg cgccagacaa aacttggttt aaagcgggct
     1741 acttcatgga ttctctttaa gatcaaggta caagacacag caaatccgat cattgtcgcc
     1801 aataaagggc cgtatacttc aaataaacga atggctgggt attgaagaag gagtttcact
     1861 agaaacccaa taaccaaata attaattgcc gccttgtttt caaacattcc ttgcagcatg
     1921 ttggaaacta gcatatatag tcctaggaag agtcctacaa aacttgcttg gatcagtaca
     1981 ttacttccta gagaatccgg tgtataaaat aatgtattta gcggataggc aagtaacata
     2041 acaccaaatg tcgccggaaa cataaaaaat gaaaacaact gtaaatttcc gcttgtcaat
     2101 tttgccagac ctactcgatc tttcaacgta acagcttctg taatcaacgg taacccagta
     2161 gacgcgatcg atgttgccaa tgcaataact accatcgtca acttatctgg attggcacta
     2221 aaaatcgaaa aaagatccag taactgtgca ttggaatact cggtggatgc agacatgatc
     2281 ttcataaaag tgaactgatc gactaatttg aagatcgtta cccctgaacc cacaataata
     2341 aacggaatcg cttctttaac ggtatctaac aacaattcct tcgttgcaat atttattttg
     2401 ttttcgctgt agtgaataaa cgccgatgta tacgcttgat gtttcttcaa aaaatacaaa
     2461 aggacaagaa tacttgctag cataccgata aatgccgcga aagttgattg cgtgaccgcc
     2521 gtcacatagt caccatccat gactttcatg atgatgaatg tagccaatag catataaaaa
     2581 atacgtgcca cctgttctac aatctgcgac agcgcataag gcatcatttc ttgattgcct
     2641 tgcaaatacc ctcgtatcac actcatacaa ggaaaaacca atacagcgat actcaaagaa
     2701 cgcatcgttg gaatcagttc ttctccgcca ccagaagcat gagctagcca aggagaggcg
     2761 atgtacatga acaaagcaaa caacaggcct aacccgccca tcatctgtaa tgcacggata
     2821 aataatcgtc gtgaagtgcc atattcattc aatgaattat agcgtgcagt ttgttttgca
     2881 atcgcagcag gtattcctgc tgtagagatc agtagaaaca atgcatatat attgtatccc
     2941 atattgaata atccattggc agccttagca ttttcgccca tccaagcata ccagggaatg
     3001 atatagacag cgcctagcaa tcttgaaatg atattactgg cggtcatcca agcagagcct
     3061 cgtgccattt tttcttgcgg tgttaattga tctaatgctg gttttctttg attatccatc
     3121 ggtcagattc caactttcta caaaacatta ctatcatttt aattggtaaa acagaaactg
     3181 gcaatcgatt ttatataact tttataagaa caattgtttt tttgaattcg gtttcgtttt
     3241 tttggtaaag taaagaaata tacagccttt tatggctaac atttatagga gatatcaatg
     3301 acacaagaaa taaaaaaact acccgttata tttttaggtc ttttctttat cacagttgga
     3361 ttgaattggt tcttgctccc tcacgatata gcttccgccg gagtgggcgc catcggacat
     3421 ttagttgaaa cggccttttc tatccaacgc tcagttactg tttggagcat caatcttatg
     3481 atgctgcttc tagccgctgt cctattagga aaatcagtat tttttaagac tgtagcggga
     3541 agtctgcttt ttcctgttgt tttagagatt gttccaaaag tggaaatgat ctcctcacat
     3601 ttcttttcac tgatttcagg aagtcttttg ttcagtcttg gggtttacac attgtacaca
     3661 gtcggggcct caaatggcgg tataacgata ccgccgatta ttttccacaa attttttcgg
     3721 cttccaatgc cgcaaggact tttacttacc aatagtctta tcgtcttttt gaattattat
     3781 gtttttggat tgttggagac actattcgtt ctcctatcta ttacagtcag ttcattgttt
     3841 atgaaaatac tcatccgctt gtcccctgtg tcaagaaaat ttgaataaag ctggattttg
     3901 tcatagcgga aacaaacgat atattaaaaa gagtgtgcta taataaacca agaaattgaa
     3961 taaagcgagg gatcaatcaa tgagcttctc tttagaagac atccgtcatt tactattaaa
     4021 agaaaatcta ttaaaagaat ttgtttcaac aagaggctgg catcttgatg taccagaaaa
     4081 agctgaattt cttcagttaa gttatgattc aagaaaagct gatgcttcta ctttgttttt
     4141 ctgtaaagga atgaatttca aagaagaata tttggattca gcaatcgaac agggtattca
     4201 atattatgta tcagaacaac catacgaaaa ttcagcagta ggaatcatcg taacggatat
     4261 ccgtaaagca atggcgcttc ttgctatggc tttttacgac tatccgcaaa ataaattgaa
     4321 agtgatcgga ttcactggaa caaaaggtaa aacaactgct gcgtacttta caaaagcaat
     4381 cttggatcac acaacaaata aaaaaacagc attgctttct actatgaata caacgttaga
     4441 tggcaaaaca tatttcaaat cccatttgac aacgcctgag tcattagatt tatatcgtat
     4501 gatgtcagaa gcagttgaaa atggtatgac tcatctagtg atggaagttt cttcacaagc
     4561 ttacaaaaca caacgcgtat acggcttgac tttagatgtc ggaatcttct tgaatatctc
     4621 gcctgaccat atcagtccga tcgaacatcc gacatttgat gactatttct attgcaagcg
     4681 acaattgatc ttgaattcaa aaacagttgt tttaaatcat gaaagtgact attttgattt
     4741 acttagggaa acagccgatc tattccagat tccaacgatc acttatggtc gttcggaaga
     4801 aagcaattat caagtgatcc gttcagataa aggtactcac ggttttactt tatcttctca
     4861 tgaagatcgc ttagctatct gcgatactgc ttatgatatt ttgctggctg gaggattcaa
     4921 ccaagaaaat gcagccagtg cgatcattgc cgcagcacta acaggtgcct ccagtcaaga
     4981 tgcacaagac ggattaaaag aagctcgtgt accaggccga atggatcaat tgatccaatc
     5041 caatggagcg catgtctatg tggactatgc acataattat ctaagcttga aaacattgct
     5101 gaaatttgcc aaaaatgaac atccagatgg ccgtgtaatt gttgtattag gcagcccagg
     5161 aaacaaagca atctcccgtc gacatgattt tggaaaagtg ctatcagaaa cagctgacat
     5221 cgcctttttg acggcagatg acccagcatt tgaagatccg aaaaaaatcg cggaagaaat
     5281 caatgaagca attactaatc cagatttgat cgttcattat gaaatggatc gtcctgaagc
     5341 gattcgacgc gctttagcag aaagcgctcc gcaagattca gtcgttattg cgggaaaagg
     5401 cgtggatccc tatcaaaaaa tcaatggtgt agatgaacct tatgaagggg attatgcaat
     5461 cgtgaagcgg ttgattgaag aataatggta tttatatact agtggtttcc catttctaag
     5521 aaaaaaaggg actgtgattt tttgtcacag ttcctttttt cttagaaatc attttaactt
     5581 ttcactagct aattctgctg ccttttctct ttcttcattt aataatttat tatcatagaa
     5641 tttgataaat gggaaccaga taatgaatgc gatgattgca caaatgactg ctaacacagc
     5701 agctttccaa tcaccaccac taccaacaaa tgctccgatc ccgactggtg aaggccacgg
     5761 catctgtgca agcatcggtt tgacgatttc cagccgaatg gcaaaataag cgagtgtagc
     5821 agaagccatc ggagctaaga aaaacggaac cgctaaatag ggattgtaga cgattggcat
     5881 cccgaaaatg attggttcat tgatattgaa taatcctggt acaagtgagg cttttcctaa
     5941 cactttcagc tgatcagatt tcgcgaggaa tgcaataaag atgatcaatc ctaaagtcgc
     6001 acctgatcca ccaacggtta cataagaatt gttgaattct cctgccaacg gataatttgc
     6061 gccttgtgcg ttagctgcca tattagctaa tacgatcggg gtcaagaaag aagtgatgat
     6121 attcgctcca tggatcccta cgatccacaa tgcatggatc aagaaataaa tcaccatgac
     6181 accaagccat gtattcgtca actgcgtaac aaacccaaat gggatagcaa tcattttgaa
     6241 gatatccgtt cctagtgcaa ctaataatcc attgatgatc atcaccacaa acgcaatgac
     6301 aaatgttggg atcagagcag tgaaagaacg agaaactcct ggcggaacaa catctggcat
     6361 ttttatgacc caatttctct tgacacacat acaatacaat tcgaccgcaa taatcgacat
     6421 aataatagct gtaaagattc ctgaagttcc taaacgttcg acaaaagagc ccatcctaat
     6481 accatttact acagtttctg tctcattgat agaagcaatc aagctgattt taccatcagt
     6541 catgatcaat tcagggatac acataaagaa tgcaaaaaca gatagtaatg cccccgtgag
     6601 cggattaaca tttaattttt gttcatctcg ttcaattgac gttaattcat aagcaaacac
     6661 aatggcaaag taaatagata aaatccccat cgtcattgta ttgacgatca tgtacaagtc
     6721 actaactttg aaaaaagttg cattaaaaaa tccttctaaa aatgggaacg gtaatggcaa
     6781 aatatttaaa accaaaaaca tcgagcctac aatcgtgaaa ggaatagaag ccatccccgc
     6841 tgccatgaca gcccgaacga ttttaagttg cgcaattttc cccataggtc ccatcaggta
     6901 tttctgcaaa aaatcaaaca ttagttattc tcctttccta aatataacgc tttttcatat
     6961 tgtttaatac ggatatactg ttggtcttgg ttgagtgaaa ttttctttaa acgatactgt
     7021 ataaatgcac tggcaaaaat accgcttaca acaagaatga aaacaaataa ctgtcctcca
     7081 ttgttatttg ccataaaagg atacaagaat gtaaatcctg aggtaaacaa cactggaatc
     7141 actaataaat taaagacact ctggaacaaa aaataccact ttgtccatgg aacgttcgtt
     7201 tggtgttttc gataaagctt tacttgttct cctactgcag gcaaaatagc taaaatcaga
     7261 atcatcggta aactacttcc taaactttta gctgatagaa gcagaatcat ccattgcatg
     7321 ttcatgaaaa agaaaaaggc agtcaggtaa cgtacgatta gataacgatt gaaatacatg
     7381 tttttcaaac taagttgctt tttttctaat tcgattttcc tttttactgt atcctgcaca
     7441 atgatccttc ctttctattt atttttttga tacaaataat tcatttcctg tgcaacttcc
     7501 aataatgtca tcgtcgtcat cagatgatct tgtgcatgga ccatgataat ttccatttct
     7561 atttttgtcc cgctggcata ttctttcaga agttgagttt gtgactgatg cgcatgaaga
     7621 atctcttcat ttgcttgtcg caacttctct tctacttctt caaacatctc tttacgcatc
     7681 gagcgaaatg cttcatgtat cagtgtccgt gcatttccgc tatggaaaat gatatcaaaa
     7741 gaagtcactt gtagtgtttc actgtttatt ttttcagcca tctgttttcc tccttattcg
     7801 atcagtttga taaatttact gctgaaattc tcaaaaatag gttcagctaa gatttcctgc
     7861 tgtactttta gctggtcaac taacctaacg attgctttag tcacgaccgt aattccttca
     7921 ttttcgacat aagaaggcga aactaaaaaa acaaaatgaa tttcttgata ttgatcccac
     7981 tgcatcccag aaggaatcaa tgcaacggct acctttgttt cgattccaac tggaacagcg
     8041 ggatgtggga ctgccactgt atcactgaat atgatttgtc ccatattttc acgttgctgg
     8101 atctgctttc gcatttctct tatatatcct tcttcttcat ttatgctcag cttttgcagc
     8161 aaatcatgaa gaatttcttc ctttttcggt tcgtacgtat aagtcttgaa aaatttttca
     8221 gacaactgtt cggtgtaaat ctgctttttt tgctgtagag aaaatgctgg tatcggtcta
     8281 tctgaggaat gatagggtat agattctcca actacctttc gtattttacg aacatcatct
     8341 tcattcaaaa aaatactgac atgtaaaact ggaattttga aaaacattgt cgaaagatca
     8401 atagaagaaa taatcaggtc gattcctttt aaagttgttt cattgatctc ataatagcct
     8461 tttacactta ccactgtaat atttttaccg aattcactga ctacgcgatt ctttaatagt
     8521 tgggcactgc cgtaacccgt tgcacagata atcaatgcat gtagtttatg cgcagctctt
     8581 tctttttcca atgccgccat gagatgcaaa gccaaatagg cccattcatc ttcattgatg
     8641 gtatagtttt tcaaagccgg catgttgctg aaatgccttt ttactgcgtc aaagactttg
     8701 ggattttctt tctttatctc ctcagtcaat gggttctcaa tgcgaatacc tcggctcaat
     8761 cgaatcatca tcggtgacaa atgatcgagt agtccattga aaagctggag atcctctact
     8821 aatgaaaaat gaaattcctg agacaatctt ttcagaactt tcgctaattc ctttgatagt
     8881 tcctgatcat tttgattttc tttgtgattc gacttagcca tgagatgtaa tgtcaaataa
     8941 gcaatttctt cttcgggaaa cgatacacct gcgattacct caatacgttg aacgatccgc
     9001 tgcgccactt gatactctac tcgcttagaa acttcttgtt cgatgcctaa ttccttgatt
     9061 tcaaaccctt ctcggagccg tttgatcccc aaagccaagt gtaaagtcag attctgtatg
     9121 ataaaatccg aaacttttaa cttagcttct cttgtttcat cgagaatgat aatgactagc
     9181 tcttcaaaac tgatgtctgg gaaaaaacgg ctttgcccta aatattcttt caatgaattt
     9241 ccataatggt ttccaaaaaa agtatccatg ataaaatgcc gtcgatttct ttcttcccct
     9301 tcgatccatg tcccttgacc atgcttagaa acgattttta gtgaatatgg aatcaatcgc
     9361 tccttgattt cttgcatgtc ctttgtcaaa ctggaacgac tgatataaag ctcttctgcc
     9421 aaatcatcca taaaaagaat ggcatcttcc aaaagcaatt ggttcaatat atacttttgt
     9481 cgatcttcgg actcataaaa tccttgcggt tctctagcat atccaggtac tatttcttgc
     9541 cgactcaaaa acaagtcaaa tgtcagtctt tgtaaaatat gcagttggta gccataacct
     9601 ggtttagcaa tgatcttacc tccgtttttt tctactaact ctttgatccg ctgcaagtaa
     9661 ttacggattg tccgatcaga taataacaat tcagaagcta aacgttgact ggtgacatac
     9721 tccttcttgt gatcaatcaa atatctcaac agcaattttt ccttctgttt cattatcatc
     9781 cactcctatt ccatcatatg ttctcttcga tcacctctgc taacttctgg atgcctgtag
     9841 ggattgggac atacgcttgg aaagggatac taacaacagg ctttccaaca gattctccta
     9901 attttttgaa tttatccaaa aacatcgtcg tttgcgggct gatcagaaat aaatcaaaat
     9961 cactattctt aatcatatta tcgccttctg tagctgaaac tgcatctacg agtacctctt
    10021 ctcttttttg cttaaagaaa tccgttgtct ttgaggccat catcgaggat gacattcctg
    10081 ccgcacaaat aatcaatgct tttttcataa atcaatcact ccttttattt ttataaacca
    10141 agcatatctt gtaagcgttt tctattcaaa atatatattt ccgtagcatt gcggaaaaaa
    10201 tgtgtatcat atcttgaata ataatttctg gcaccgtgat atactagtga tagaattttt
    10261 tcaggagggg atttagtgag tatcacagtg aatattttgt atacaagtac aaatggacaa
    10321 gccagaaaat ttgcagaaga aatggtcgaa agaaacattg tcaaagcgat tcgtgcagaa
    10381 gaaggaaacg agaaatatga ctattacttt ccactagaag atccagaatc tcttttactg
    10441 atcgaccgtt ggaaagatga agaagcaatc gaaaagcatc acaaatccga aatgatggct
    10501 caaataaacg aactccgtaa aaagtataaa ttaaaaatga aagtcgaacg tttcactgat
    10561 ttaccgtaaa aagatacaca tggaatgata gcaatttatc atccatgtgt atcttcactt
    10621 tttccttatt ttttattcga tctcatctaa tgattcaaac cgtgtttttc tcttattttt
    10681 cggataagta tttcagtcac cccaatatac atgattccta aacatacccc aaggatattc
    10741 tcaaaaatgc ggatcgttac tgctcctgga accccgaata aactggcagt gattgccaaa
    10801 gcaccaaaag aattaaaaac tgttttatat ctataggtag aacaaacacc taaagctaga
    10861 ccacctaata taccgaccca gttaaaagga ataaattggc tgataccaat aaataaaaca
    10921 catccaatca atgagccaat gattcggtct actgctcgtt ctttgaaacc aatcgacatc
    10981 aatccatagc tagatacaat agaagaaaat gcaaaccctg cccacatcaa tcgctgaaaa
    11041 gggacatact ctcctgcaac gatcaaaaga ctaatgccaa atgcatagat gagcatccaa
    11101 attttttcct gcgaatacat gcctttcaaa aaaatctctt taaacaagct tttgtctttg
    11161 ttcttttctc gatgttttct gtaaaggaga actgaaaacc aacaaaaaaa caaaaataat
    11221 aagctacttg tttgtgtaaa gaaattcttg ttcaacgaat ctttaggtaa cgaataaaca
    11281 ataaagagat atgaaaagcc gtacaaagaa gcattcccca ttttgggatc actagcggtt
    11341 gccattaata tgctgcttag taaaataaaa tgaatcaaca tgcccataac tgaccatttg
    11401 atcagctgga catacggtgc tactaatagt gagaacatga cgattgctaa acctatgatc
    11461 gaatgggaaa tcttataacc aaagtcaaca aaacgaatac tcatcaacat acagaataaa
    11521 accacagcgt atggtttatg cttcgattca aataatgttg taattataat gataaaacaa
    11581 atggcaaaca atacgatgaa taatgatcgt aaaaacaatg aacaaatata catgcacttt
    11641 tcttttttag aagctgcttc tcgtattttt tgttttaaaa taaacggatc tagttgtaga
    11701 gactgataaa aatccacctg ttgtcgtcct ttctttttac ttttattttt tacagtcatc
    11761 tatgacaggt tgtaaatact atggaacatc atatggcgtt gtcattttct tgtcaactat
    11821 ttttcctatt catgttttta taaaaaaaca tatcgttttc ttgtatgatt aaatcaccac
    11881 aaaataaaca actgagagaa cgatatgctt tatgaatggt tctatcaaaa aaattctgag
    11941 atcaatagac aaaaatttga tgattacaga ggtctcttac gaaaccttac agaagaaaaa
    12001 aacattgatc gttgctgcag ttctcttccc tgctccatgt gtctgtagaa attgtggttc
    12061 tgctgtggta gatggaaata ggaaattaat ggtggtgaaa aacaagaaga aggaaaccat
    12121 tgtccacttt gaataatata atcatatgcc aatggttata cgcctaaaaa agcagcgcta
    12181 tacctgtaaa aactgtcgaa gccattggaa cgctcaaagt tattttatcc gtccaaggca
    12241 ctcgatttca aatcatgtca gacataaaat cacttcttta ctgactgaaa aagtgtcgtt
    12301 attttttatt tcgaaaagtt gttaggtatc tttgacaacc gttattcgca cattgaaaga
    12361 gttgagaagc tatttaccaa agtcttccaa aagaattctg tcttgagtgt tgagggttgt
    12421 gaatttcgtt ctcacgtttc cgttgaggat aaactgagtt ttatttccgc ggatggcgag
    12481 acagaaaaat taatcgagaa aatttaccta cacaaaaaat tcctcgatta acaaactatt
    12541 tccttagctg cactaatcct aaagaaatag aattcttagt tacagacatg aacgccacct
    12601 actttcagct caccaaacat gttctaccaa atgcgaaatt ggtgattgat cgattacttg
    12661 gtttttccac gcctttcaag gaggcgtaac ctttattcac gagttggttg aagctttccg
    12721 agacaaaaga cccgacttgt ttttctcctt attgacaaag agccattaaa actagagaaa
    12781 aaattacaac tgatcgctga ttatttgtag aaagaaaatc aaagtattac ccaataaact
    12841 tgagtcatta ggcaaaaaac actgcgaaac tcaagaatga ttttcgcagt gtttttgtat
    12901 attttttatt aactagtatt actacaaagg tttatttttc acttctcaac gcagcacaaa
    12961 attgtcttta cttatttact ggttgggttc aaccttctct actccgatac taatatttgt
    13021 ttgtataccc cttctaaaat cggtagtttc cattcaattg atgggtacta gttgatttta
    13081 aaaactatgc ttattcaatt ttttatttta gctcatatat ttcgtttctt tctaaatttt
    13141 atactgatga aaacaagtag cacacccaat attgacaata taaactggct aatgatggtt
    13201 tctccagttt ttggaagatg cttatttcca ctgcttgttg gattgtttcc tcttatatct
    13261 gagctatcag caacttttct attctctttt gttaaaaaga tcgccacttc ttgttctttt
    13321 tcgatcttga aagttactgg tgtcgcatcc aattcatacc ccgttggcgc ttgagtttct
    13381 accagctgat atgctcctgg tgctaagcca tctatcgcca gttttccatc tgctcctgtg
    13441 gttagacctg actgcagaac ggttccattg gcatcttgta actcaaaaac agctccttgc
    13501 aggacttctc cgctttggtc atcgatcttt gtcaacacga caccgcctgg tgtaagtcga
    13561 ttttctttcg tcaattctac caccgctgtc tgacttcttt cgatctcaaa ctcgattggt
    13621 gtcgcatcca attcataccc cgttggcgct tgggtttcta ccagctgata cactcctggt
    13681 gctaagccgt caatcgccag ttttccatct gctcctgtgg ttagacctga ctgcagaacg
    13741 gttccattgg catcttgtaa ctcaaaaaca gctccttgca ggacttcccc gctttggtca
    13801 tcgatctttg tcaacacgac accgcctggt gtaagtcggt ttgctttcgt caattctacc
    13861 accgctgtct gacttctttc gatctcaaac tcgatcggtg tcgcatcta
//
LOCUS       DAZKLM010000072        13808 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3764
            SAMEA115706913-rid22582563.denovo.074, whole genome shotgun
            sequence.
ACCESSION   DAZKLM010000072 DAZKLM010000000
VERSION     DAZKLM010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706913
            Sequence Read Archive: ERR13196647
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13808)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13808)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287823.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287823.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,004
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,849
            CDSs (with protein)               :: 2,849
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 41 of 96
            Pseudo Genes (incomplete)         :: 52 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 15 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13808
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706913-rid22582563.denovo.074"
                     /isolate="VRE3764"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(162..2159)
                     /gene="tkt"
                     /locus_tag="ACG842_002018"
     CDS             complement(162..2159)
                     /gene="tkt"
                     /locus_tag="ACG842_002018"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HFQ0248548.1"
                     /translation="MFDKIDQLGVNTIRTLSIEAVQKANSGHPGLPMGAAPMAYALWT
                     KHLKVNPTTSKNWADRDRFVLSAGHGSAMLYSLLHLAGYQVTIDDLKQFRQWDSKTPG
                     HPEVHHTDGVEATTGPLGQGIAMAVGMAMAEAHLAATYNKENFNVMDHYTYAICGDGD
                     LMEGVSQEASSMAGHMKLGKLIVLYDSNDISLDGPTSKAFTENVGARYEAYGWQHILV
                     KDGNDLEAISKAIEEAKAETDKPTLIEVKTVIGYGAPKEGTSAVHGAPLGEDGIKMAK
                     EVYGWEYPDFTVPEEVAARFHQTMIEEGQKAEDAWNEMFANYKKAYPELAQQFEDAFD
                     GKLPENWDAELPTYEVGSSQASRVSSKEVIQELSKAIPSFWGGSADLSGSNNTMVAAD
                     KDFTPEHYEGRNIWFGVREFAMASAMNGIQLHGGTRIYGGTFFVFVDYLRPAVRLAAI
                     QHTPVVYVLTHDSVAVGEDGPTHEPIEQLASVRCMPGVQVIRPADGNETRAAWKVAME
                     TTDAPTILVLSRQNLPVLPSTKEVADEMVKKGAYVLSPSQGETPEGILIATGSEVDLA
                     VKAQKELAEKGKDVSVVSMPSFDLFEKQSSEYKESVLPKSVKKRVAIEAAASFGWERY
                     VGTEGATITIDHFGASAPGTKILEEFGFTVENVVNTYNQLS"
     gene            complement(2261..2512)
                     /locus_tag="ACG842_002019"
     CDS             complement(2261..2512)
                     /locus_tag="ACG842_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 family protein"
                     /protein_id="HFQ0248549.1"
                     /translation="MLSPEKIERINELARKKKENGLSKAEEQEQINLRKEYLEAFRSG
                     MRHHIEGMKVVDPEGNDVTPEKLKEIQKKKGLHNRNDNF"
     gene            2659..3282
                     /gene="lexA"
                     /locus_tag="ACG842_002020"
     CDS             2659..3282
                     /gene="lexA"
                     /locus_tag="ACG842_002020"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HFQ0248550.1"
                     /translation="MARQTESRQIEVLRYIHEQVTQKGYPPTVREIGEAVQLSSTSTV
                     HGHLARLEKKGFIQRDPTKPRAIELTKQGLEKIGIRPTTIPVLGVVTAGEPILAVEDA
                     SDFFPIPPNLSSEEGSLFMLTIRGDSMINAGILDGDNVIVRKQETAQNGDIVIAMTAE
                     DEATCKRFYKEKNFFRLQPENDLLEPIILDQVSILGRVVGLYRSHIY"
     gene            3462..3680
                     /locus_tag="ACG842_002021"
     CDS             3462..3680
                     /locus_tag="ACG842_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0248551.1"
                     /translation="MKSMRRLGLVLTVLFLGIIIGNFGIRPALLVFVPLFTLWFMLWD
                     EKKYRQSERRRIHEDHFYHEPYYRSRQS"
     gene            complement(3969..4481)
                     /locus_tag="ACG842_002022"
     CDS             complement(3969..4481)
                     /locus_tag="ACG842_002022"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HFQ0248552.1"
                     /translation="MDLKDYIASIPDYPSKGIVFRDISPLMADGDAYREATKQIVNYA
                     KEKRIDMVVGPEARGFIVGCPVAYELGVGFAPVRKKGKLPRETIEVTYGLEYGTDTLT
                     LHKDAIKPGQRVLICDDLLATGGTIKATIELIEQLGGVVVGCAFLIELMDLHGRDKIK
                     GYDIITLMEY"
     gene            complement(4495..6792)
                     /gene="recJ"
                     /locus_tag="ACG842_002023"
     CDS             complement(4495..6792)
                     /gene="recJ"
                     /locus_tag="ACG842_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008409 - 5'-3' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HFQ0248553.1"
                     /translation="MRQANFDWKLSETEPSESFLQMIRELKLSPFVGKLLWQRGYHEE
                     KDINRFLHPKEQELHDPYLIHDMDKAISRIQEAVISGEKILVYGDYDADGITSATVMK
                     ETLELLGAEVEVFLPNRFEHGYGPNQSVYQEKIEEGIQLIITVDNGVAGNDAVAYAQQ
                     AGVDVIITDHHELPENLPDAYAIVHPRHPAGHYPFPDLAGVGVAFKVASALLEEPPAE
                     FLDLVAIGTIADLVSLTDENRILVSLGIDAIHHSERIGLQALFEESGVKMRDADETTI
                     GFSIAPRLNAIGRMGDPRPAVSLLATFDEEEASVQAKKLNEINEERKAIVEQITQEAL
                     AMVNKENHIHLLVNRGWHEGVLGIVAGKIMNETGKPTLVLTLKEDGSAKGSGRSIEAL
                     NLFEMLDQMRDLFTYFGGHHAAVGLTMPSENVTILQEKMNQYIVDHQIDLMRGPELRI
                     DEVLLPNEVTVERIDELKLLAPFGTDNPLPQFLFRQVQAENIKRIGANQQHLKFVLSD
                     ASSQLDAVAFGFGTQEEELLNNQVDVVGKLSINEWNGRKKPQLMVSDFSVGGLQVFDW
                     RAKRFREQSRTSEGSLYLAFDSSTLKFVPEIIQEAAIVFEGLDSVKAIIEKRKPNSLV
                     VMDCPDDLADLKEVFRSYSFNRVYLMGISPDEAYLNGVGTREQYAKLFKLIHSQERID
                     IRHKIKAIAQYLRIPEKLLIFMIQVFFELKFVTIENGVLQKVASPESHPLTESRLYQR
                     RLNKIKVEEFLLLSDIPTIKKWLTT"
     gene            complement(6934..7377)
                     /locus_tag="ACG842_002024"
     CDS             complement(6934..7377)
                     /locus_tag="ACG842_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapA
                     domain-containing protein"
                     /protein_id="HFQ0248554.1"
                     /translation="MKELKKQSNVILGFVLVLIIVLFAVLNNKNVPVSFGFTSFSAPL
                     ILVIIGSALIGALIVFLTASATLWQQKKQIKQLTQEMSEYQTELQKKIEEAKEEQQRE
                     FRNERAELEAAYQAELQAKTAQISQLKAPEEQKTQSPKQSFNYFD"
     gene            complement(7378..8166)
                     /locus_tag="ACG842_002025"
     CDS             complement(7378..8166)
                     /locus_tag="ACG842_002025"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HFQ0248555.1"
                     /translation="MNLEDKVVLVTGSSGGLGAQICYEAAKQGAIVISCARRMAFVEG
                     VRDECRRLSGKEAYAFKVDVSNPESVDELYEKVMEEVGRVDILVNNAGFGIFEDFLTF
                     DLGKAYDMFEVNILGMMVLTQKFAIDMAERRQGHIINIASMAGKMATAKSTVYSATKF
                     AVLGFSNALRLELKPLGVAVTTVNPGPIETNFFDKADPSGSYLEKVGQIVLEPAKLAK
                     MIVRNMRHPKREINQPFILEVASKFYTLFPTIGDYLASGIFNKK"
     gene            complement(8163..9104)
                     /gene="rnz"
                     /locus_tag="ACG842_002026"
     CDS             complement(8163..9104)
                     /gene="rnz"
                     /locus_tag="ACG842_002026"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - RNA endonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HFQ0248556.1"
                     /translation="MELQFLGTGAGVPAKHRNVTSIALKLLDERNEVWLFDCGEGTQM
                     QILRTTIRPRKIGKIFITHLHGDHIFGLPGLISSRSFQGGDTPLEIYGPKGIEEYIKV
                     SLGISQTRLSYPLKFIELNETAPIFTDQQFSVYTKKLNHGIDSFGYRVVEHDHKGELQ
                     VDRLKELGIPAGPLYGKLKQGETIQLEDGRTINGKDFVGPDKKGRIVTILGDTRKTHN
                     SVVLAENSDILVHESTFNKDEARMAHNYFHSTTHQAAEVAKEAQAKRLLLTHISARYL
                     GKAALELEKEAQEVFENTTIMKDFDSIEIPFREEEEA"
     gene            complement(9281..9868)
                     /locus_tag="ACG842_002027"
     CDS             complement(9281..9868)
                     /locus_tag="ACG842_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1269 domain-containing protein"
                     /protein_id="HFQ0248557.1"
                     /translation="MSKRVIILNFQVESQAYQAFSEIKKLHAVRSIKGEQMAVVSHDP
                     NGTHQFKIEDFIDFTGNNKSSTGGLIGMLVGVLGGPLGILLGWFAGGMIGATQDAKEI
                     KDAQTVFDFVGNKIGEGATGLILIADEEDNRPLNQLVMMELGGEITRLDYEDVEKDVH
                     DAKEAENHAKEAAQKTWKDKHPQTSNDNESSGSSN"
     gene            complement(10089..11402)
                     /gene="obgE"
                     /locus_tag="ACG842_002028"
     CDS             complement(10089..11402)
                     /gene="obgE"
                     /locus_tag="ACG842_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003126661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HFQ0248558.1"
                     /translation="MSMFLDQVTIDVKAGKGGDGMVAFRREKYVPDGGPAGGDGGRGG
                     DVILIVDEGLRTLMDFRFNRHFKAQPGENGMSKGMHGRGSEDTYVKVPQGTTVRDAET
                     GALLGDLIENGQTLVVAKGGRGGRGNIRFASPRNPAPEIAENGEPGQERKIELELKVL
                     ADVGLVGFPSVGKSTLLSVISSARPKIGAYHFTTLVPNLGMVTTSDGRSFAAADLPGL
                     IEGASQGVGLGTQFLRHIERTRVILHVIDMSGMEGRDPYEDYLAINKELSTYNLRLLE
                     RPQIIVANKMDMPDAPENLVKFKEQLNKEKEDEFADDIPVFPISGVTRQGLDALLNAT
                     ADLLEVTPEFPLYEEELEEETVHYGFNPEGPEFQIDRDSDATWILSGEKIEKLFQMTN
                     FDHDETVMRFARQLRGMGVDEALRARGAKDGDLVRIGEFEFEFVE"
     gene            11690..12700
                     /gene="gap"
                     /locus_tag="ACG842_002029"
     CDS             11690..12700
                     /gene="gap"
                     /locus_tag="ACG842_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HFQ0248559.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIKEVSDDIEVVAINDLTSPTMLAQ
                     LLQFDSTHGTYPGTVTATEDSIVVDGEATRVYAEPDASKIPWAKENGVDIVLECTGFY
                     TSEEKAKAHLDAGVKRVVISAPAGAMKTIVYNVNDDTLDENDKIISAGSCTTNCLAPM
                     AYFLNNEFGIEVGTMTTVHAYTSTQMLLDGPVKGGNLRAARSAADNTIPHSTGAAKAI
                     GLVIPELQGKLQGHAQRVPVVDGSLTELVSVLKTKVTADQVNEAIKKHTVDNPSFGYD
                     DRQIVSSDVIGTTQGSIFDPTQTEVTTAGDFQLVKTVAWYDNEYGFTCQMIRLLEKFA
                     NL"
     gene            complement(12785..13720)
                     /locus_tag="ACG842_002030"
     CDS             complement(12785..13720)
                     /locus_tag="ACG842_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFQ0248560.1"
                     /translation="MKLWMKILLGVALALIVALVFAGNYFYNYAVVPSEKDFLDEDTP
                     GTTQTSKETDAHKWFSSKENRSDWTITSKDGLKLSAIYLPAETKSEKTALVAHGYMGN
                     AETMTNYAKMFHDMGYNVLVPDARGHGKSEGDYIGFGWPERKDYVQWINKVLENNGKS
                     QEIVLYGVSMGAATVMMTSGEKLPDNVKAIIEDCGYSSVHDELAYQLDDMFSLPAFPL
                     MQVTSLVTKVRAGYFFGEANAVEQLKKNQRPMLFIHGDEDTFVPFEMLDKVYRATKGP
                     KEKYVVKGAEHAEAYKTDPEKYQQVVQQFLSQYVH"
BASE COUNT         3808 a         3096 c         2435 g         4469 t
ORIGIN      
        1 ctcaagaaac acaaactaat ccgaagaatt ctttgttttg tcatttaaat gtaacatagc
       61 cgaaaacgaa aagtaatatt gatttattct tctaacacat aaagaacagc tgtctccaac
      121 ttgcagacag ctgttcttat cagtcaaaca attgcttgga tctatgatag ttgattgtac
      181 gtattcacga cattttctac tgtaaatcca aattcttcta aaattttagt gccaggggca
      241 gatgcaccga aatgatcgat tgtaatcgtt gcgccctctg ttcctacgta gcgttcccat
      301 ccaaatgatg cagcagcttc gatagccaca cgtttcttca ctgatttagg aagtacactt
      361 tctttatatt cagaagattg tttttcaaag aggtcaaagc ttggcataga aacaacagaa
      421 acatctttgc ctttttcagc caattctttt tgagctttca cagccaaatc aacttcagaa
      481 cctgttgcaa tcaaaatacc ttctggtgtt tctccttgtg atggagaaag aacatacgcg
      541 ccttttttca ccatttcatc tgctacttct tttgtagaag gcaatactgg aaggttttga
      601 cgactcaata caaggatagt aggcgcatct gttgtctcca tcgctacttt ccaagcagca
      661 cgtgtttcat ttccgtctgc cggacgaatc acttgaacac caggcatgca gcgaacagat
      721 gctagttgtt caatcggttc gtgcgttggt ccgtcttctc cgacagcaac cgagtcatgt
      781 gttaagacgt aaacaacagg agtatgttgg attgctgcta aacgaactgc tggtcttaga
      841 taatcgacaa agacaaagaa tgttccaccg tagatacgag ttccgccatg aagttggata
      901 ccattcatag ccgaagccat tgcaaattcg cggacaccga accagatgtt gcgtccttca
      961 taatgttcag gagtgaaatc tttatctgca gccaccattg tattattgga acctgacaag
     1021 tccgctgaac caccccagaa gcttggtata gctttcgata attcttggat gacttcttta
     1081 cttgaaacac ggcttgcttg actgcttcca acttcataag ttggcaattc tgcatcccag
     1141 ttttctggta gtttgccatc aaaagcatct tcaaattgtt gtgctaattc aggataagct
     1201 tttttataat tggcaaacat ttcattccat gcatcttcag ctttttgtcc ttcttctatc
     1261 atcgtttgat ggaagcgggc agctacttct tctggaacag tgaaatctgg atactcccaa
     1321 ccataaactt ctttagccat cttgatgcca tcttcaccaa gaggagcacc atgaacagca
     1381 gatgttcctt ctttaggagc accatagccg atgactgttt ttacttcgat caatgttggt
     1441 ttgtctgttt cagcttttgc ttcttcaatc gctttagaga ttgcttctag gtcatttccg
     1501 tctttcacta agatatgttg ccagccatat gcttcatagc gagcaccaac gttttctgtg
     1561 aaagcttttg aagttggacc gtctagtgaa atatcgtttg aatcgtataa aacgatcaat
     1621 ttccctagtt tcatatgacc tgccatcgaa cttgcttctt gagatacccc ttccatcaag
     1681 tctccgtcac cgcatattgc atacgtatag tgatccatca cattaaagtt ttctttgtta
     1741 tacgttgctg caaggtgcgc ttctgccatt gccataccga cagccattgc aatcccttgt
     1801 cctaatggcc ctgtagtggc ttctacacca tctgtatgat gtacttcagg atgtcctggt
     1861 gttttagaat cccattgacg gaattgtttc aaatcatcga ttgtcacttg atagccagct
     1921 aaatgaagca agctatacaa cattgctgac ccatgtcctg ccgaaagaac gaaacggtcg
     1981 cggtcagccc agttttttga tgtcgttgga tttactttca ggtgttttgt ccataacgca
     2041 tatgccatag gtgctgcgcc catcggcaat ccaggatgtc ctgagtttgc tttttggact
     2101 gcttcaatac ttaatgtacg aattgtgtta acgccaagtt ggtcaatttt gtcaaacaaa
     2161 ataacccctc ctattaattt aggttttttt tacctttatt tcttacactt tcattctata
     2221 agatgtcaca cgaaaaagca atctttttca gtatttttta ctaaaagttg tcatttcggt
     2281 tgtgtaagcc ttttttcttt tggatttctt ttaatttttc cggggtcacg tcattgccct
     2341 caggatcgac gactttcatt ccttcgatat gatgacgcat accggaacga aaagcttcca
     2401 aatactcttt gcgcaagttg atttgttctt gttcttcggc ttttgagaga ccattttcct
     2461 tctttttacg tgcaagttca ttgatccgct ctattttttc aggtgacagc atgctcagcc
     2521 ctccttttac tctccttcat attatataaa aactgaaaaa aaaacaacta gataaactaa
     2581 ataaaatacg aacattcgtt tgatttagcg ctttttatat ggtactattt atttatcaag
     2641 tgaaagaagg tgtaccttgt ggcccgacaa actgaatcaa gacaaatcga agtcttacga
     2701 tatatccatg aacaagtaac ccaaaaagga tacccgccga ctgtccgtga aatcggggaa
     2761 gctgttcagc tttcttctac ttctactgtt cacggtcatc ttgcacggct tgaaaaaaaa
     2821 gggtttatcc aaagagatcc gacgaaacct cgtgcaatcg agttgacaaa acaaggttta
     2881 gaaaagattg ggattcgtcc aacaactatt ccagtacttg gcgtcgtaac agctggagaa
     2941 cccattttag cagtagagga tgcttcagac ttctttccaa tccctcctaa tctttcttcc
     3001 gaagaaggca gtctctttat gctgacaatt cgtggagata gtatgatcaa tgctgggata
     3061 ttggatggtg acaacgtgat cgttcggaag caagaaactg ctcaaaatgg cgatattgtc
     3121 attgcaatga ctgccgagga cgaagcgact tgcaaacgat tctataaaga aaaaaacttt
     3181 ttccgtctgc agcctgaaaa cgacttgctc gaaccaatca tcttagatca agtatctata
     3241 ttaggacgag tagtaggact ttatcgtagt catatctatt aattgctact aattaaaaga
     3301 aaacttttta aaaagagttt gagacgttgg tctcaaactc ttttttcgcc cgggttcttc
     3361 ttctttcaaa tttttatcac ttccctcttt acaaaccgaa caaatattcg tatactaaat
     3421 atacgaacaa ttgttcgcat aaattaaaga ggtgagaatt catgaaatca atgagacgtc
     3481 ttggcttagt attaactgtt ttgtttttag gtattattat tggtaatttc ggcatacgtc
     3541 cagctttact tgtgtttgtt ccgttattta ctttgtggtt catgttgtgg gatgaaaaga
     3601 agtaccgtca atctgagcgg cgcagaattc atgaagatca tttctatcat gagccctact
     3661 acagatccag gcaatcataa taaggattga ctgctaatta ttcaaaagac aagaagttaa
     3721 acttttacgg gggtgtgaca aaaatcggtc acatcctttt tttcgaataa acggtgttct
     3781 aaagccagtt tttcggtatt aagtcaaatt gcggaaaaag tgatttttta aacttctact
     3841 ggtctttgct tgtttccatt tataaaaaaa tagcgaccct caaaagtcag atagagaatc
     3901 tgacttttga gggtcgcttt aaaatcgctt ctttaagaat aacatcaaga atagcttctt
     3961 ttttctgctt aatattccat taatgtaatg atgtcatatc ctttgatttt gtcgcgtcca
     4021 tggagatcca tcaattcgat caaaaatgcg cagcctacga ctacaccgcc taattgttcg
     4081 atcagttcga tcgtcgcttt gattgttcct ccagtagcta gcaaatcgtc acaaatcagc
     4141 acacgttgac ctggtttaat ggcatcctta tgcaatgtca atgtatctgt tccatattct
     4201 aatccataag tcacttcgat ggtttctcga ggtaacttcc cttttttgcg gactggggca
     4261 aaaccgactc ctaactcata agcaacagga catcctacga tgaatccacg ggcttctggc
     4321 ccaactacca tatcaattcg tttttctttt gcataattta cgatttgttt agtagcttcc
     4381 cgataagcgt caccatctgc catcagtggg gaaatatctc gaaacacaat tccttttgac
     4441 ggatagtcgg gaatactggc aatataatct tttaaatcca ctctttgttt cctcctaggt
     4501 tgttaaccat ttctttatgg taggaatatc gctcaaaagc aaaaattcct caactttgat
     4561 tttattgagc cgtcgctggt ataatctgct ctcagtcaaa ggatgacttt ctgggctagc
     4621 gactttttgt aaaacaccgt tctctattgt aacaaatttc aattcaaaaa acacctgtat
     4681 cataaaaatt aataattttt cagggatacg caaatattga gctattgctt ttattttatg
     4741 acgtatatca attcgttctt gagaatggat caatttaaac aattttgcgt actgttctct
     4801 tgtgcctacc ccattcaaat aggcctcatc aggtgaaatc cccatcagat aaacacgatt
     4861 gaatgaataa gaccgaaaaa cttctttcag atccgctaaa tcatctggac aatccataac
     4921 aactagagag tttggttttc ttttttcaat tattgccttt acagaatcta gtccttcgaa
     4981 aacaatcgca gcttcctgga taatctcagg cacgaatttc aacgtcgaag aatcaaatgc
     5041 taaataaagg ctaccttccg acgttcggct ctgttcacgg aaacgttttg cccgccaatc
     5101 aaatacttgc agaccaccca cagaaaaatc agataccatc agctgcggtt tcttacgccc
     5161 gttccattca ttgatcgaaa gctttcctac cacatctacc tgattattca gcagttcttc
     5221 ttcttgagta ccaaaaccaa aagcgaccgc atcaagctga ctcgatgcat cggaaaggac
     5281 aaatttcaga tgctgttggt ttgctccgat tcttttgata ttttctgctt ggacttgtcg
     5341 aaataaaaac tgaggcaaag gattatccgt accaaatggc gccaatagtt tcagctcatc
     5401 aattcgctct actgttactt catttggcag caatacttca tcaattctca actcaggccc
     5461 tctcatcaag tcaatttgat gatcgactat atattggttc atcttctcct gtagtatagt
     5521 gacattttca cttggcatcg ttaaaccgac tgctgcatga tgtcctccga agtaagtaaa
     5581 taaatctcgc atctggtcaa gcatttcaaa aagattcaag gcttcaatac tacgtccaga
     5641 tcctttagct gatccatctt cttttaaagt aaggacaagc gttggtttac ccgtttcatt
     5701 cattattttc ccagcaacaa tgcccaaaac gccttcatgc catcctcggt ttaccaatag
     5761 atggatatga ttctccttat tgaccatcgc taatgcttct tgtgttatct gctctacgat
     5821 tgcttttctt tcttcattga tctcattcaa tttttttgct tgaacggatg cttcctcttc
     5881 atcaaacgtc gcaagtaacg agacggcagg acgtggatcg cccatccgac cgattgcatt
     5941 tagtctggga gcaatcgaaa aaccgatcgt tgtttcgtct gcgtccctca tttttacgcc
     6001 gctttcttca aatagcgcct gaagtcctat ccgttctgaa tgatgaatgg catcgattcc
     6061 taatgaaaca aggattcgat tctcatcagt caatgaaact aaatcggcga tcgttcctat
     6121 cgctactaaa tccaaaaatt ctgcaggcgg ttcttccaat aatgcagaag ctactttgaa
     6181 tgctactccg actcctgcca aatctggaaa tggatagtgc cccgctggat gacgagggtg
     6241 aacaatcgca taagcgtctg gtaaattctc aggtagttcg tgatgatctg taatgatcac
     6301 gtctacacca gcttgctgag cgtatgctac cgcatcattt cctgctaccc cgttatcaac
     6361 agtgatgatc aactggattc cttcttcgat cttttcttgg tataccgatt ggtttggtcc
     6421 atacccatgc tcaaatcgat ttggcaggaa tacttctact tctgcaccaa gtaattccag
     6481 tgtttctttc attaccgttg cactggtgat cccatctgcg tcataatcac cgtaaacgag
     6541 gattttttct ccactgatta cagcttcttg gattcggctg attgctttat ccatatcgtg
     6601 tatcagatac ggatcatgta attcttgttc ctttggatgt aaaaatcggt tgatatcctt
     6661 ttcctcatgg taacctcttt gccacagtaa cttaccgaca aatggagata atttcagttc
     6721 acggatcatt tggagaaagg attcagaagg ctctgtctcg cttaacttcc aatcaaaatt
     6781 tgcttgtctc actttctcac tcctaactta gttattatag caaaggaaat cagcaaaaaa
     6841 aagcagaaag aaagaataaa aacagatcag atccgctaga cagatgttca gcaggacctg
     6901 atcttttgta cgaccattta tgaaatatcc ctattagtca aaataattga agctttgttt
     6961 gggcgattga gttttttgtt cttccggcgc tttcaattga ctgatttgtg cggttttcgc
     7021 ttgtaactcc gcctgataag cagcttccaa ctccgcacgt tcgtttctaa actcgcgttg
     7081 ctgttcttct tttgcttctt caattttctt ttgaagttct gtttgatatt ctgacatttc
     7141 ctgagtcagc tgtttgattt gctttttttg ctgccatagt gttgccgaag cagtcaaaaa
     7201 tacaatcagt gcaccaatca gcgcagaacc gatgatcact aagatcaatg gcgcagaaaa
     7261 ggaagtgaag ccaaaactta caggaacatt tttattgttc aatacagcaa aaagaacaat
     7321 aatcaaaact aaaacaaatc caagaatcac attgctttgc tttttcaatt ccttcaccta
     7381 ctttttgtta aaaattccgc tggctaaata atcaccaatc gttggaaaca gcgtataaaa
     7441 ttttgatgca acttctaaaa taaacggttg attgatttcc cgttttggat gtctcatatt
     7501 gcggacgatc atttttgcca acttggcagg ttctaaaacg atttgtccga ctttttccaa
     7561 atagcttccg cttggatcag ccttatcgaa gaaatttgtc tcgattggac ctggattcac
     7621 tgtcgtaaca gctacgccaa gtggtttcaa ttctaatcgc aaagcgttgg agaagccaag
     7681 gactgcaaat ttcgtggcag aataaaccgt tgattttgca gttgccattt ttccagccat
     7741 agaggcgata ttaatgatat gaccttgtct tctttctgcc atatcgatcg caaatttttg
     7801 tgtcaaaacc atcattccta agatgttgac ttcaaacata tcgtatgctt ttccaaggtc
     7861 gaatgtaagg aagtcttcga aaatcccaaa acccgcattg tttaccagaa tatcgacgcg
     7921 cccgacttct tccatcacct tttcatataa ttcgtccacg ctttcaggat tgctgacgtc
     7981 taccttgaaa gcataagcct ctttgccgct caagcgtctg cactcgtctc tgacaccttc
     8041 tacaaatgcc attcttcgtg cgcaacttat aacgattgct ccttgctttg ctgcttcata
     8101 acagatttgt gcgcccagac cgcctgaact acctgttact aaaacaactt tatcttctaa
     8161 attcatgcct cttcctcctc gcgaaatgga atctcaatgc tatcaaaatc tttcataatc
     8221 gtcgtgtttt caaacacttc ttgtgcttct ttttcaagct ctaaagctgc ttttcctaaa
     8281 tatctcgcac tgatatgagt aagtagcagc cgttttgcct gcgcttcttt tgcaacttca
     8341 gcagcttgat gagtcgtgga atggaaatag ttgtgcgcca ttcttgcttc atctttatta
     8401 aatgtgcttt catgtaccag aatatcgcta ttttccgcaa gcacaacgga attatgtgtt
     8461 tttctcgtat cgcctaaaat agtcacgatt cgtccttttt tatctggacc tacgaaatct
     8521 tttccattga tcgtccgccc atcttctagt tggatcgttt caccttgctt cagtttgccg
     8581 tacagtggtc cagctggaat acctaattct tttagacgat ccacctgcaa ttctccttta
     8641 tgatcatgtt caaccacacg ataaccaaaa ctgtcgatcc catgatttag tttttttgta
     8701 taaacagaaa attgctgatc agtaaaaatc ggggctgttt catttagctc aatgaatttc
     8761 agcggataac ttaaacgcgt ttgcgaaata cctaaagaga cttttatgta ttcctctatc
     8821 ccttttggcc cgtaaatctc taatggcgta tccccacctt gaaatgaccg gctgctgatg
     8881 agcccgggaa gtccaaaaat atgatcgccg tgtaaatgtg taatgaaaat tttaccgatt
     8941 tttctcggac gaatagtcgt tcgcaaaatc tgcatttgcg tgccttcgcc acagtcgaac
     9001 aaccaaactt cattgcgctc gtctaaaagt ttcaatgcta tacttgtcac atttctatgt
     9061 tttgcaggaa cacctgcacc tgttcctaaa aattgtaatt ccataatcta cctgactttc
     9121 tttaagttcc tctttaattt tagggtaaaa agaagggata atcaaccgta agtcgagaat
     9181 gggttcaaat taaaaataac gttctaaagc gatagcgttt tctttgaaaa ttactataaa
     9241 aacagcagcg aacccgtttg agatcctctg ctgtttttta ttaatttgaa gatccagaac
     9301 tttcattgtc gttggaagtt tgcggatgtt tgtccttcca agtcttttgt gcagcttctt
     9361 tcgcatgatt ttcagcttct ttcgcatcat gcacatcttt ttcgacatct tcataatcca
     9421 agcgagtaat ttctccgcct aattccatca ttaccaattg attcagtggg cgattgtcct
     9481 cttcatcagc aatcaagatc agtcctgtag ctccttcacc gattttattt cctacaaagt
     9541 cgaacacggt ctgggcatct tttatttctt tggcatcttg tgtcgcaccg atcattcctc
     9601 cggcaaacca tcctaataaa atccctagcg gaccacctaa tacaccaacc aacattccga
     9661 ttaatccacc cgtagaagat ttgttattac cggtaaagtc gataaaatct tctattttga
     9721 attgatgtgt cccattagga tcatggctga caactgccat ctgttctcct ttaatcgaac
     9781 gaacagcatg taactttttg atttctgaaa atgcctgata tgcctggctt tctacttgaa
     9841 aatttaaaat gattactcgt ttgctcatta aaaacgcccc tttcatacct tcttgttatt
     9901 tattttacct ttaattgaaa aataagaaaa gaaaagcact ctttttttcc aataaaatag
     9961 ggctgtgaca aaagtcgtat cacagctcct ttttccgaat aaacggcgtt cttgaagctg
    10021 atcacttctg tcacaacctc ccttaaatct gaatctatat tttttggtat ttgtttttat
    10081 ttgcttgcct attcgacaaa ttcaaattca aactcaccga tacgtacaag gtcgccatct
    10141 ttcgcaccgc gtgcccgaag agcttcatcc acacccatac cacgtaattg acgagcaaaa
    10201 cgcatcactg tttcatcatg atcgaaattc gtcatttgga ataatttttc gatcttctca
    10261 ccagataaga tccacgttgc atctgaatca cgatcgattt ggaactcagg accttctgga
    10321 ttgaatccat agtgtacagt ttcttcttcc agttcttctt cgtacaatgg gaattctggc
    10381 gttacttcta acaaatctgc tgtagcattc aataacgcgt ccaatccttg tcgagtcact
    10441 ccagaaattg gaaatactgg gatgtcatca gcaaattcat cttctttttc cttgttcagc
    10501 tgttctttga atttcactag attttccggt gcatccggca tatccatttt gtttgccacg
    10561 atgatttgcg gacgctctag caagcgtaag ttataagtag ataattcttt gttgatggca
    10621 agatagtctt cataaggatc tcgtccttcc atcccactca tatcgatcac atgtaaaatt
    10681 actcgtgttc tttcgatatg acgaaggaat tgtgtaccca gcccaacacc ttgcgaagct
    10741 ccttcaatca agcctggcaa gtctgctgcg gcaaaacttc tgccatcact tgtagtaacc
    10801 attcctagat taggtacgag cgtcgtgaaa tgataagcgc cgatttttgg acgagcagat
    10861 gaaataacgg atagtaaagt cgatttcccg acagatggaa aacctacaag tccaacgtct
    10921 gccaatactt tcaattctag ttcgattttt cgctcttgtc ctggttcacc gttttcagca
    10981 atttccggtg caggattccg aggagaggca aaacggatat tccctcgtcc accgcgtcca
    11041 ccttttgcca caactagtgt ttgaccattt tcaattaaat ctcctaaaag agcaccagtc
    11101 tccgcatctc ggacagtggt tccttgtggt actttaacat aggtatcttc tgaaccacga
    11161 ccgtgcatcc ctttactcat cccattttca cctggctgcg ctttgaaatg gcgattgaac
    11221 cgaaaatcca tcaatgtacg taacccttca tcgacgatca agatgacatc gccgccacga
    11281 ccaccgtctc cgccagccgg tccgccgtca ggaacgtatt tttcgcgacg aaatgcaacc
    11341 attccgtctc cgcccttgcc agctttcaca tcgatcgtta cttgatctaa aaacatggac
    11401 ataattaagt cctcctatta ttcatttatt tatttgactg cctgaatttc cacaataaac
    11461 aaaagtctta cttattttat cggtgaaatc tgattttgtc tatcatttca gcaaaaagat
    11521 tgtttacctt cttctttttt tatcatttgg taaggtttcc aacctttttt ctgctaccaa
    11581 gtataatttt ttgacaaaat attctttgcg tagcagaata acagtgtatt aattgtatta
    11641 attgaggatg ccgtttcaca gcacccctaa taaaggagga acttttgata tgacagtaaa
    11701 agtaggtatt aacggatttg ggcgtattgg ccgtctggct ttccgccgca tcaaagaagt
    11761 ctcagatgat attgaagtag tagcaatcaa cgacttaaca agtccaacta tgttagcaca
    11821 attattgcaa tttgactcta ctcatggtac ttatcctggc acagttactg caacagaaga
    11881 ctcaatcgta gtggatggcg aagcaactcg tgtttatgca gaacccgacg caagcaaaat
    11941 cccttgggca aaagaaaacg gtgtagatat cgtactagaa tgtactggtt tttatacatc
    12001 tgaagaaaaa gcaaaagcgc atttagatgc tggtgtcaaa cgagtagtta tttctgcccc
    12061 agctggtgct atgaaaacca tcgtctataa cgtcaatgat gatacattag atgaaaacga
    12121 caaaatcatc tcagcaggtt catgtacaac aaactgttta gcacctatgg cttatttctt
    12181 aaataatgaa tttgggatcg aagttggtac aatgacaaca gttcatgcct acacttccac
    12241 acaaatgctc ttagatggac ctgtaaaagg cggtaatttg cgtgctgctc gttcagctgc
    12301 tgacaatact atcccacatt ctactggtgc tgcgaaagca atcggcttag ttatccctga
    12361 gctacaagga aaattacaag gacacgcaca acgtgttcca gtcgttgatg gttcattaac
    12421 tgaattggtt tcagtattga aaacaaaagt aactgctgat caagtaaacg aagcaatcaa
    12481 aaaacacaca gttgacaatc cttcattcgg gtatgatgac cgtcaaatcg tttctagcga
    12541 tgtcattgga acaactcaag gttcgatctt tgacccaacg caaacagaag taacaactgc
    12601 tggtgatttc caacttgtta aaactgttgc atggtatgac aatgagtatg gatttacttg
    12661 ccaaatgatt cgcttactag aaaaattcgc taacttgtaa tttgatagat caatcgataa
    12721 aagaagcctt tgactggatc cagtcaaagg cttctttctt ttcatcccct acttttaaac
    12781 tcgttcaatg aacatattgg cttaaaaatt gttggactac ttgctgatat ttttcaggat
    12841 ctgtcttgta tgcttccgca tgctcggctc cttttacaac atacttttct tttggtccct
    12901 tcgttgcacg atagacctta tctaacattt caaatggtac aaatgtatct tcgtctccat
    12961 gtataaagag cattgggcgc tggtttttct tcaattgttc cactgcattt gcttcaccga
    13021 aaaagtagcc agcacggact tttgtcacta gactcgttac ttgcataagc ggaaatgctg
    13081 gaagactaaa catatcgtct agctgataag caagctcatc atgaacggaa gaataaccgc
    13141 aatcttcaat gattgctttc acattatctg gtagtttttc tccgctagtc atcataactg
    13201 ttgctgctcc catgctgaca ccgtatagaa cgatttcttg cgattttcca ttgttttcta
    13261 gcactttgtt tatccactgt acatagtctt tacgctctgg ccatccaaat ccaatgtaat
    13321 ccccttcgct ttttccatgt cctcttgcat caggaacaag gacattgtaa cccatatcat
    13381 gaaacatctt tgcgtaattg gtcatcgttt cagcgtttcc catatagcca tgagccacaa
    13441 gagctgtttt ttcacttttc gtttcagcag gaagataaat cgctgataat ttcagaccgt
    13501 cttttgaagt gatggtccaa tcgcttcggt tttcttttga actaaaccat ttatgtgcat
    13561 ccgtttcttt ggatgtctgt gttgtaccag gtgtatcttc gtctaaaaag tctttctcag
    13621 aaggcacaac agcataatta taaaaataat tccctgcaaa cactaacgca acaatcagag
    13681 ctaaagcgac gcctaaaagg attttcatcc aaagtttcat aaatcatcca tctcatttct
    13741 cttttttcat ataaccttga attagtataa tcaaagaatc tctactatgc aatgttcatt
    13801 tttataac
//
LOCUS       DAZKLM010000073        13429 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3764
            SAMEA115706913-rid22582563.denovo.075, whole genome shotgun
            sequence.
ACCESSION   DAZKLM010000073 DAZKLM010000000
VERSION     DAZKLM010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706913
            Sequence Read Archive: ERR13196647
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13429)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13429)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287823.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287823.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,004
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,849
            CDSs (with protein)               :: 2,849
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 41 of 96
            Pseudo Genes (incomplete)         :: 52 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 15 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13429
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706913-rid22582563.denovo.075"
                     /isolate="VRE3764"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..914)
                     /locus_tag="ACG842_002031"
     CDS             complement(<1..914)
                     /locus_tag="ACG842_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent cysteine synthase family protein"
                     /protein_id="HFQ0248561.1"
                     /translation="MIISTITEAIGSTPLYRFDNQHYSMPKGSAIYAKLEYLNPGGSI
                     KDRLGSYLIKEGFAQGKINEKTTIIEPTAGNTGIGIALAALTYKLRTVFVVPEKFSLE
                     KQQLMKALGAKIIHSPSEQGIIGAISKSKKLAEEISNSYLPLQFENKDNPAAYYHTLG
                     PEIFHEIKENIHSFVAGIGSGGTFAGTSTFLKEKYPDIRIIGVEPEGSVLNGGDPAPH
                     EIEGIGVEFIPPFLSPLSINQIETISDVEGFNYTRQLAREQGLLVGSSSGAAFAAALR
                     EIRRLPPGHRVVTIFPDAADRYLSKNIY"
     gene            1342..1905
                     /gene="efp"
                     /locus_tag="ACG842_002032"
     CDS             1342..1905
                     /gene="efp"
                     /locus_tag="ACG842_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HFQ0248562.1"
                     /translation="MISASDLRAGMTFVQDGKLIKVLDASHHKPGKGNTVMRMKLKDV
                     RSGATYDTTFRPEEKFEKAHIDTKPVQYLYTMDDTAFFMDLETYEQYEIPVETVEEEM
                     KYILENMEVKIQFFGSEVIGVQLPTTVVLRVVETQPSIKGATVTGSGKPATMETGLVV
                     NVPDFIETDELLEINTAEGSYVKRASK"
     gene            2006..2176
                     /locus_tag="ACG842_002033"
     CDS             2006..2176
                     /locus_tag="ACG842_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0248563.1"
                     /translation="MKNKKVFDFIYILKDEWQKLEKEKTALIYEEIEMIHKIENIRTI
                     PNYIEAKIFFHH"
     gene            2391..3650
                     /gene="lysA"
                     /locus_tag="ACG842_002034"
     CDS             2391..3650
                     /gene="lysA"
                     /locus_tag="ACG842_002034"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HFQ0248564.1"
                     /translation="MTELIIGGVTASTLAEKYKTPLYVYDEEKMRQTMLSFKRGFVSK
                     RFDTKVLYASKAFQTIEMLHLVREYGFGLDVVSGGEIYTALQADFPRDTIYFHGNNKT
                     PEELIYALENDLLHIVADNLMEVELLAELSQRYLKKICIMLRLNVGVEAHTHEYIVTA
                     HIDSKFGMSYESKECQQALQLIKESSYLELEGFHAHIGSQIFDVTAWLAEIDKLTNYL
                     TDFDQPLSLNLGGGFGIRYTQNDQPIPVEEALKKLIEYTEEALEKRKLTIRQLLIEPG
                     RSIVGEAGTTLYTIGFIKKTPHKKYYFVDGGMTDNIRPALYQAEYDCDLAAKLSEEKT
                     EKVTIAGKMCESGDVVIKEAYLPKAESGDLLAVYSTGAYGYSMSSNYNRATRPAVVFV
                     NNGQSRLIVRRQAFSDLLRGEVSYDPS"
     gene            3637..4437
                     /gene="dapF"
                     /locus_tag="ACG842_002035"
     CDS             3637..4437
                     /gene="dapF"
                     /locus_tag="ACG842_002035"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HFQ0248565.1"
                     /translation="MILHKYNGCGNDFILLDYAEDTDYSQLAASLCTKEKYDIDGLIA
                     VKIDPLEMIYYNKDGSRAPMCGNGIRCFARYVNDQNMMNEQEFNVETLAGTRKITVLD
                     PSPFYCSVDMGVPDDSPEKIKANQTYPIKNQEIEINGEKVTITSLFLGTIHTVVFVEN
                     AREEVWRKRGELICHHPLFKEKTNVNFVEVVNEQELIVRTYERGVGWTLACGTGCCAS
                     YVVAKETGKISADQVVVHLEQGDLMISGEDAIQMAGPAVHEWTRILEE"
     gene            4440..5321
                     /gene="dapA"
                     /locus_tag="ACG842_002036"
     CDS             4440..5321
                     /gene="dapA"
                     /locus_tag="ACG842_002036"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HFQ0248566.1"
                     /translation="MIKGSIVALITPMNEEGSVDYAGLEKLIQFHLDEQTDGLLVLGT
                     TGESSTLTQSEEEQILQLTVKKVAGRVPVIAGAGTNNTKETIEKAKHFASLGADALLV
                     ITPYYNKTSDAGLAAHFTAIAEASPIPLILYNVPSRTGMSIPIHVLVNLAEHPNIIGL
                     KEASGDMAYVMDAARLIGEEFFLYSGNDDLILPVMSVGGSGVISVWANIQPKIVHELV
                     KDTQDGRWQQAKEKQLNALELIHALFSETNPIPVKAAMSLLDLPSGPLRLPLVSLSKE
                     KKKQLAQLLLKERTVEK"
     gene            5318..6034
                     /gene="dapB"
                     /locus_tag="ACG842_002037"
     CDS             5318..6034
                     /gene="dapB"
                     /locus_tag="ACG842_002037"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HFQ0248567.1"
                     /translation="MKIGLIGSGQMGNRVEEVAQERGDTVLLLRTAPKEATTLAFTEL
                     PELLIDFSHPDNLEKILSYSLSYQLPLVIGTTGYTQSQFQEIKEHAKHVPVMYSANFS
                     FGIMVMNQLIKQAAAMLQGWQIELIEKHHSRKKDAPSGTANMLLQTIQEVQKLQPVYN
                     WQGKKREENQIGVHSIRAGSLPGEHDVLFAATDEIFTIKHESFSNRIFAAGAVEAADW
                     LKDQSPGYYKLEDLLMDKGV"
     gene            6040..6735
                     /gene="dapD"
                     /locus_tag="ACG842_002038"
     CDS             6040..6735
                     /gene="dapD"
                     /locus_tag="ACG842_002038"
                     /EC_number="2.3.1.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047200 - tetrahydrodipicolinate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HFQ0248568.1"
                     /translation="MEAQEIIRYIQTASKKTPVKVYLNVTEPIEFKESKVFGEGSSFV
                     VFGDYETIAPVLEAESEKIIEIEIETAARYSAVPLLDIKKINARIEPGAIIRDQVSIG
                     NNAVIMMGAIINIGAVIGENTMIDMGAVLGGRATVGKNCHIGAGAVLAGVIEPASAKP
                     VIVEDGVLVGANAVIVEGVHIGKDAVVAAGAVVLEDVAAETVVGGIPARVLKISDDQT
                     KENTALIAALREL"
     gene            6751..7752
                     /locus_tag="ACG842_002039"
     CDS             6751..7752
                     /locus_tag="ACG842_002039"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HFQ0248569.1"
                     /translation="MREIDTCIVGATGLVGQTMLKVLEEYDFPVGRLKLLASKRSAGK
                     VCVFKKKKHTVQELTETSFEGYDLALFSAGASVSKEFAPIARSQGLFVIDNSSAWRED
                     SDIPLIVPEVNLEDYTLNRLIANPNCSTIQAVLPLKALQEAFGIQRVNYSTYQAVSGS
                     GQKGIEDLHRTQNDEEPVLYPYNISQTVIPEIDQPLENGYTKEEQKMINETRKILHQP
                     ALPVSATCVRVPIENGHGVSVAVQLQKEFTTEEIRECLSQFPGIQLEDDLAAHKYPTS
                     ILARGTDMVYVGRIRKDTSLENGLLFYTTADNIRKGAAANAVQIAAALMQKIAEVAR"
     gene            7749..9110
                     /locus_tag="ACG842_002040"
     CDS             7749..9110
                     /locus_tag="ACG842_002040"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HFQ0248570.1"
                     /translation="MSNTLKVAKFGGTSMSHEEQFKKVRTIVLSDPNRQVIVVSALGK
                     RTSDDDKVTDLLYLIYAYLQHHVDWTPLWKRICDRFIQVRDVLALQLPIEDELARIQK
                     QLEDEKITEDYLVSRGEYLTAQLMAEYLGYQFIDAKDLIFFDYDGKIDQEKTAQEITK
                     AFKRYPKMVVPGFYGANPAGKEKLLGRGGSDITGAILAKMLQAESYENWTDVSGIMMA
                     DPRIIDHPKIIEEISFRELREMAYMGANVLHEAAVFPVQEADIPIQIKNTNDPFAQGT
                     KISNHEIEKENGLTGIAGRKDFLSITLFKRHMSDEIGFIWKAMSIFAKHEISIEHIPS
                     GIDNIGVIVSAEAIADKLFLITKELKETLGVEEIVVIEDLALISVVGGPHKELIGLSG
                     KVLSILNKLEIRTSILSQGAQELNLIIGVPNNQYETVVKGIYEGMVNTDAERISNGTP
                     SNS"
     gene            9070..10221
                     /locus_tag="ACG842_002041"
     CDS             9070..10221
                     /locus_tag="ACG842_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HFQ0248571.1"
                     /translation="MLKEYRMALHQIPEIGFHEFKTKKYLYEQVKNSEAVIHEIGETG
                     LLLYFDNQQEETIAFRTDIDALPVKEETNLSFASKHEGYMHACGHDGHMAMLLGLTDF
                     ITKHIEDLIYNVVLIYQPSEEISGGAQSVIQSGLLDKYEVQAIFGFHLWPGLAKGEVF
                     SRPGPLMAQSSETDIIIKGQSAHIASSEKGIDSLEAAVRFLKNVYDFDRQLPAEWNHL
                     MKFGQIQGGTIRNVLAGEVIVSGSIRSYSSDMQNYLKRGLDHLAEQFHSTATISFRYN
                     DGYPAVINHRGLYAALLDSNKLHELDAPVLQAEDFGIYTQQYPCVFFFLGVGDTPALH
                     DPTFDFDMDVLENGVEWYKTILFTENLLVKIDSSSKEERVNNDEFIQSI"
     gene            10196..11365
                     /locus_tag="ACG842_002042"
     CDS             10196..11365
                     /locus_tag="ACG842_002042"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HFQ0248572.1"
                     /translation="MMNLSNRFSPRLSRIEVSKIRQFDQQISSIPDVIKLTLGEPDFP
                     TPEHVKQAGIAAIEEDFSHYTGMRGLEELREAACIFQQQRYGLTYDPQTEVLTTVGAT
                     EAIASALLSVLEEGDKVLIPAPAYSGYQPLVELAGAELIPIDTSDTGFVCQPEQFERA
                     FEQYGSAVKAVILNYPNNPTGTTLSAKQLKAIAEVLKKYPVFVISDEVYAELTYSGTH
                     MSIATYLPEQTIVISGLSKSHAMTGWRVGFIFAQKALIDELIKVHQYLVTAATTMSQK
                     AAAEALINGVKDSENMKEQYQTRRDYLVQQLAPLGFEVTQPNGAFYLFCKLPAAIQTD
                     SWQFCVELAEQAKVACIPGIAFGPEGEGYIRISYASSMEKLHEACKRIKDFLTEQ"
     gene            11540..12919
                     /locus_tag="ACG842_002043"
     CDS             11540..12919
                     /locus_tag="ACG842_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFQ0248573.1"
                     /translation="MEKIKVVIVGASHGGHQSILELLTRYGENVDITLFEAGDYISFM
                     SCGMELYLEDKVTDVNDVRNFRPENFPQSNVHILNNHEVKTIDADKKTVTVTSASDNE
                     TKEYPYDKLILSSGVKPNNLPVPGADLENVYLMRGYNWATKIKEKLNDPAIKKIAVVG
                     AGYIGIEAAEASRKAGKEVVLLDVIDRPLGTYLDAEMTDILEQHLNENGIEVLTNAKI
                     EAFTGNGKVEAIKTSEKEIPADLVIQAAGVKANTEWLKGIVDLDERGWIQTNEYLQTN
                     LPDVYAVGDATLAYSIPARKKMPIALATVARREARYVVKHLFEEVPNKPFGGVVGSSA
                     LSVFDYHFAASGLNSFTAAKAGVDVRVSYYEDTLRPKYVPEEQGNPKVSIQLFSDPMT
                     HQLLGGAVLSTYDVTGQGNVLALAIQNKLTLEELAEADFFFQPGFDRQWSMLNLAAQQ
                     ALGEEPFVE"
     gene            13200..13429
                     /locus_tag="ACG842_002044"
                     /pseudo
     CDS             13200..13429
                     /locus_tag="ACG842_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         4484 a         2339 c         2924 g         3682 t
ORIGIN      
        1 aagtaaatgt tttttgataa atagcggtct gctgcgtctg gaaaaatcgt aacgacacga
       61 tgaccaggtg gtaaacgacg aatttctctt aatgctgctg caaaagccgc accactcgaa
      121 cttccaacta atagtccttg ttctcgtgct aactgcctag tatagttaaa cccttctaca
      181 tcagagattg tctcgatctg attgattgaa aggggagaaa gaaacggcgg aatgaattct
      241 acaccgatac cctcaatttc gtgaggggct ggatcaccac catttaacac agagccctct
      301 ggttctacac cgattattcg aatgtcggga tatttttctt tcaaaaaggt agaagttcca
      361 gcaaaggttc caccactccc aattcctgct acaaaactat ggatattttc tttgatttca
      421 tgaaagattt ctggccctaa cgtatgatag taagcagcag gattatcttt attttcaaat
      481 tgaagaggca gatagctgtt tgatatctcc tctgcaagtt ttttactttt gctgatggca
      541 ccgatgattc cttgctcact aggagaatgg atgatctttg ctcccaatgc tttcatcaat
      601 tgttgttttt ctaagctaaa cttctctgga acaacaaaaa ccgttcttaa tttgtatgtg
      661 agtgcggcca gagcgatccc aattcctgta tttccggcag ttggttcgat gatcgtcgtt
      721 ttttcattga tttttccttg ggcaaatcct tctttgatca aataactgcc caatctatct
      781 ttgatacttc ctccaggatt taaatattct agttttgcat aaatagcaga acctttaggc
      841 atagaatagt gctgattgtc aaagcgataa agcggtgtgg aaccaatagc ttctgtaatt
      901 gtcgagataa tcatgtgggc ctcctaaaaa aagataataa aaaaaggagc tgactattaa
      961 atcagctcct tgaaagacat agagaaaaat tgagtagata aaaagataag ttacctggta
     1021 ctcattctat gaagggcaat taatagacat gacaaatagt atgctgcttt tatgcaccat
     1081 acaacagcaa cgattcaatt gtttttggtt gctgactgtc atgtgtatcc ctccaagtaa
     1141 aatttttatt aacgatagca tgaaaaaaaa taaaagtcaa aggcttgaaa gaaaaaaata
     1201 tttcgatagc atcgtttacc cgtgtttatt tttctgacaa ataggcatga gaccttaaaa
     1261 gatagagaat actctagttt tctggccggt tgtctgatac aataaagttg attatgataa
     1321 agaaaagaag gagcaaaaat aatgatctca gcaagtgatt taagagccgg tatgactttc
     1381 gtacaagacg gcaaactgat taaagttctg gatgcaagcc accataaacc tggtaaagga
     1441 aatacagtta tgcgtatgaa actgaaagat gtccgttcag gtgcaacata cgacacaacg
     1501 ttcagaccag aagaaaaatt tgaaaaagca catatcgaca caaaacctgt acaatatttg
     1561 tacacgatgg atgatacagc tttcttcatg gatttggaaa catatgagca atatgaaatt
     1621 cctgtagaaa cagtagaaga agaaatgaaa tatatcctag aaaacatgga agtaaaaatc
     1681 caatttttcg gtagcgaagt catcggcgtt caattaccaa caactgtcgt attacgtgtt
     1741 gtggaaacac aaccttccat caaaggtgca acagtcactg gttcaggtaa gccagcgaca
     1801 atggaaactg gcttagtagt caatgttcct gacttcatcg aaacagatga attgttggaa
     1861 atcaatacag cagaaggttc ttatgtaaaa cgtgcgtcaa aataattcgc taacggattg
     1921 ataagaattt gaaaacagag cttgacctct tgaaaggcag atcatcggat ctgcctttca
     1981 agaggttttt tgtggaatca gcaaattgaa aaataaaaaa gtatttgatt ttatttatat
     2041 tttgaaagat gaatggcaaa agctagagaa agaaaaaact gcacttattt atgaagaaat
     2101 tgaaatgatt cataaaattg aaaatatccg aactattccg aactatattg aagcgaaaat
     2161 attttttcat cactaaatag tgttgaattt atgtttttat gtataaatag cttctaatag
     2221 ttcgggtatt ttgtttttat aaccattttt attcttattt ttaaaaaaat atttcgaaat
     2281 atactagaca attcagaaaa ttttgtttaa tattattcca ataaaattca aatctttatc
     2341 aaatgaaatt ccaataaaaa cggaaaaaaa gtgaaaagag gaatgaaaaa atgacagaat
     2401 tgataattgg cggagtcaca gcaagcacat tggcagaaaa gtataagaca cctctgtatg
     2461 tgtatgatga agaaaagatg agacaaacga tgctgtcatt caagcgaggc tttgtatcga
     2521 aacgttttga tacaaaagtg ctttacgcct ctaaagcatt ccaaacgatt gagatgctgc
     2581 atttagttcg tgaatatgga ttcgggctag atgttgtcag tggtggggaa atctataccg
     2641 ctttgcaagc tgattttcct agagatacta tctacttcca tggtaacaac aagacgccag
     2701 aggaactgat ctatgcactt gaaaatgatt tgttgcatat tgttgccgat aatttgatgg
     2761 aagttgaatt attagcagaa ttaagccaac gctatttaaa aaaaatctgt atcatgcttc
     2821 ggttgaatgt cggagtagaa gcacatactc atgaatatat cgtcactgcg catattgatt
     2881 caaaattcgg catgagctat gaaagtaaag agtgccaaca agcccttcag ttgataaaag
     2941 aaagcagcta tctagagtta gaaggatttc atgctcatat cgggtcacaa atttttgatg
     3001 ttactgcttg gttagctgaa atcgacaaat tgacgaatta cttaaccgat tttgaccaac
     3061 ctctttcctt gaatcttggc ggagggtttg ggattcgtta tacacaaaat gatcagccga
     3121 tcccagtcga agaggcacta aaaaaactga ttgaatatac ggaagaagct ttggaaaaac
     3181 gaaaactgac cattcgtcaa ttactgatcg aaccgggtag aagtattgtt ggagaagctg
     3241 gtacaacact atacacgatt ggtttcatca aaaagacacc acataaaaaa tattactttg
     3301 tagatggcgg gatgacagat aatatccgtc cagctctgta tcaggcagaa tacgattgtg
     3361 atttagcagc aaaactgtcg gaagaaaaaa cagagaaagt gacgattgca ggaaaaatgt
     3421 gtgaatcagg cgatgtggta attaaagaag cttatttgcc gaaagcagaa tcgggtgatc
     3481 ttttagctgt ttattccacg ggagcttatg gatactcgat gagcagcaac tacaatcgag
     3541 cgacacgacc agcagttgtt tttgtcaata atggccaatc aagactgatt gttcgcagac
     3601 aagcattttc tgatctacta aggggggaag tttcctatga tccttcataa atataatggc
     3661 tgcggaaatg attttatctt attagattac gcagaagaca cagattatag tcaattagcc
     3721 gcttctttat gcacaaaaga aaaatatgat atagacggat tgatcgccgt aaaaatcgat
     3781 ccgttggaaa tgatctatta caacaaagat ggcagccgcg cgccgatgtg cggaaatggc
     3841 attcgctgtt ttgctcgata cgtaaatgat cagaacatga tgaatgaaca ggaattcaac
     3901 gtagagacat tagcaggcac aagaaaaatt acggtgttgg atccctcacc tttttattgc
     3961 agcgtagata tgggagtgcc agatgattct ccggaaaaaa tcaaagctaa tcaaacttat
     4021 ccaataaaaa accaggaaat agagatcaat ggtgaaaaag taacgatcac tagtttattt
     4081 ttaggaacga ttcataccgt tgtttttgtt gaaaatgcgc gtgaggaagt ctggagaaaa
     4141 agaggtgaac tgatctgtca tcatcctttg ttcaaagaaa aaaccaatgt caactttgta
     4201 gaagtagtca atgaacaaga attgatcgtc cgtacctatg aaagaggagt aggttggaca
     4261 cttgcttgcg gtacaggttg ctgtgcctct tatgtggttg ctaaggaaac tgggaaaatt
     4321 tcagcagatc aggtagttgt tcacttggaa caaggagact tgatgatctc tggtgaggat
     4381 gcgatacaaa tggctggccc agcagttcat gaatggacaa gaatattgga ggaatgaaga
     4441 tgataaaagg ttcgatcgtg gcgctgatca cgccgatgaa tgaagaggga tcagtagatt
     4501 atgctggttt ggaaaaattg atccaattcc atttagatga acaaacagat ggattattag
     4561 ttttaggtac aacaggagaa tcttctacgc tcacacaatc tgaggaggag cagatccttc
     4621 aactgacagt aaagaaagtg gctggccgtg tgccggtgat tgcaggagct ggaacgaata
     4681 atacgaaaga aacgattgaa aaagcgaagc attttgcttc tttgggggca gacgcacttt
     4741 tggtcatcac cccttactac aacaaaacaa gtgatgcagg actagctgct cattttacag
     4801 caattgcaga agcctcaccg attccgctta ttttgtataa tgttccttct agaacaggca
     4861 tgtcgattcc aatccatgta ttagtaaatt tagcagaaca tcctaatatc attggtttga
     4921 aagaagcctc aggagatatg gcttatgtga tggatgccgc tcggttgatt ggagaagaat
     4981 tcttccttta cagtggaaat gatgatttga tcctaccagt aatgagtgta ggaggaagcg
     5041 gtgtaataag tgtatgggcg aatatccaac ctaaaatagt ccatgaatta gtaaaagata
     5101 cgcaggacgg aagatggcag caagcaaaag aaaagcaatt aaatgcactt gaactgatcc
     5161 atgccttgtt cagtgagacc aatcctattc ctgtcaaagc agccatgtca ttattagact
     5221 taccttccgg acctctacgc ctgcctttag tttctctttc aaaagagaaa aagaaacaat
     5281 tagctcagct tttgttaaaa gaaaggacgg ttgaaaaatg aaaatcggac tgattggcag
     5341 tggacagatg ggaaatagag tcgaagaagt tgcacaagaa agaggagata cggtccttct
     5401 tttacggacg gcacctaaag aagcaactac gcttgctttt acggaactgc cagaactgct
     5461 tattgatttt tctcatccag ataatttaga gaaaatcctt tcgtatagct tatcatatca
     5521 acttccgtta gtcattggca cgacaggtta tacacaatca cagttccaag aaataaaaga
     5581 acatgccaaa catgtacctg tgatgtatag tgctaatttt tcgttcggca tcatggtgat
     5641 gaatcaacta atcaagcaag cagctgcaat gttgcaaggc tggcagatcg aactgattga
     5701 aaaacatcat agtcgtaaaa aagatgcacc atctggtact gcaaatatgc tgcttcaaac
     5761 aatccaagaa gtgcaaaagc tgcagcccgt atacaactgg caagggaaaa aaagagaaga
     5821 aaaccaaatt ggtgtacaca gtattcgtgc aggttcttta cctggtgaac atgatgtatt
     5881 gtttgctgct actgatgaaa tatttacgat caaacatgaa tctttttcta atcgaatctt
     5941 tgctgcaggc gcagtagaag cagcggactg gttgaaagat cagtcaccag gttattacaa
     6001 attagaagat ttattaatgg acaaaggggt gtaaaaaacg tggaagctca agaaatcatc
     6061 cgctacattc aaacggcatc taaaaagacg cctgtgaaag tctatctgaa tgtaaccgag
     6121 ccaatcgaat ttaaagaaag taaagttttt ggtgaaggaa gcagcttcgt tgtttttggt
     6181 gactatgaga cgattgctcc ggttttagaa gctgaatcag aaaaaatcat tgaaatcgaa
     6241 atcgaaactg ctgcaagata cagcgcagtt cctttgctag atattaaaaa aattaatgct
     6301 cgtatcgaac caggtgctat tatcagagat caagtgtcaa tcggcaacaa tgccgtgatc
     6361 atgatgggag cgattatcaa tattggtgca gtaatcggtg aaaacacgat gattgatatg
     6421 ggtgctgtct taggtggacg agcgacagtg ggcaaaaact gccatattgg tgcaggagct
     6481 gtcttagcag gtgtgatcga accagcatcg gccaaaccgg ttatcgtaga agacggcgta
     6541 ctggtaggtg cgaatgcagt gattgttgaa ggtgtccata tcggaaaaga tgcagtcgtg
     6601 gcagcaggag cagtggtttt agaagatgtg gcagcagaaa ctgttgtcgg tggaatacct
     6661 gcacgtgtat taaaaataag tgatgatcag acaaaagaaa atacagcatt gattgctgcg
     6721 ttacgcgaat tatagaggag atggagaaaa atgcgcgaaa tagatacctg tattgtgggg
     6781 gcaaccggat tagtcggcca aacgatgctc aaagtgttag aagaatatga ttttccagta
     6841 ggacgattga aattattagc ttcaaaacgt tcagccggta aagtttgtgt attcaagaaa
     6901 aagaaacaca ctgtacagga attgacagaa acttcatttg aaggatatga tttagcgcta
     6961 ttttctgcag gtgcttctgt atccaaagag tttgcgccaa tcgcacgaag tcagggacta
     7021 tttgtcatag ataattcctc agcatggcgg gaagattcgg atattccatt aatcgttcca
     7081 gaggtcaatc tggaagacta tacattgaat cggctgattg caaatccaaa ttgttcaacc
     7141 attcaagcgg tacttccgct taaagcttta caagaagcct ttggtatcca gcgagtcaat
     7201 tattcgacct atcaagcagt ttctggttcc gggcaaaaag gcattgaaga tcttcatcgt
     7261 acgcaaaatg acgaggaacc agttttgtat ccttataata ttagccaaac ggtgattccg
     7321 gaaatcgatc agcctttgga aaatgggtat acaaaagaag aacagaaaat gataaatgag
     7381 acccgtaaga ttctgcatca gcctgctttg ccagtatcag caacctgcgt acgtgtgcca
     7441 atcgaaaatg gacatggagt ctctgtggca gttcaactgc aaaaagagtt tacgacggaa
     7501 gaaattagag agtgtctcag ccagtttcca gggattcaat tagaagatga tctagcagca
     7561 cataaatatc cgacgagcat tcttgctaga ggaacagata tggtttatgt tgggaggatc
     7621 agaaaagaca cttctttaga gaacggattg ttgttctata cgactgccga taacatacgc
     7681 aaaggagccg ctgcgaatgc cgtacagatt gctgcagctt taatgcagaa aatagcggag
     7741 gtggcaagat gagtaatacg ctgaaagtag cgaaattcgg cggaacatcg atgtctcatg
     7801 aagagcaatt caaaaaagtc cgtacaattg ttctttcaga tcccaatcgc caagtgatcg
     7861 ttgtttctgc tttaggcaaa cgtacgtcag atgatgacaa agtaacagac ttactttatt
     7921 tgatttatgc ttaccttcag catcatgttg attggacacc cctttggaaa aggatttgtg
     7981 atcgtttcat tcaagtaaga gatgttcttg ccctgcagtt acctattgaa gatgaactag
     8041 cacgcataca gaaacaactg gaagacgaaa agataactga agattattta gtaagtagag
     8101 gtgaatatct gactgctcaa ctgatggctg agtatcttgg ttaccagttt attgacgcaa
     8161 aagacctgat tttctttgac tatgatggga aaatcgatca ggaaaaaaca gcacaggaaa
     8221 tcacaaaagc atttaaaaga tatccgaaaa tggttgttcc agggttttac ggggcgaacc
     8281 ctgcaggaaa agaaaaatta ttgggccgtg ggggaagcga cattaccggc gcgatactgg
     8341 cgaagatgtt gcaagcggaa agttatgaga attggacgga tgtttcagga atcatgatgg
     8401 ctgatccgcg aatcatcgat catcctaaaa tcatcgagga aatctctttt agagaactga
     8461 gagagatggc ttatatgggg gctaacgttc ttcatgaagc agctgttttc ccagttcaag
     8521 aggctgatat ccccatccaa ataaaaaata ccaatgatcc tttcgctcaa gggacaaaaa
     8581 tatcgaatca cgaaatagaa aaggaaaatg gactgacagg aatagctgga cgcaaggatt
     8641 ttctttccat cactttattc aaacgtcata tgtccgatga aatcgggttt atctggaaag
     8701 caatgagcat ttttgctaaa catgagatat caatcgaaca tattccttca gggatcgaca
     8761 atatcggagt gatcgtctca gcagaagcga ttgccgataa gttatttctc atcacgaaag
     8821 aattaaaaga aacacttggc gtggaagaaa tcgtcgtgat cgaagactta gctttgatct
     8881 ctgtcgttgg gggtccgcat aaagaattga ttggattatc tgggaaagtg ttatcgatat
     8941 tgaacaaatt agaaattcgc acatcgatct tatcacaagg agcacaggag ttgaatctga
     9001 tcatcggtgt accgaataat caatatgaaa cagtagtgaa gggtatctat gaagggatgg
     9061 tgaatacgga tgctgaaaga atatcgaatg gcactccatc aaattcctga aatcgggttt
     9121 catgaattca agacaaaaaa atatttatat gaacaagtaa aaaacagcga ggccgttatc
     9181 catgaaatcg gcgaaacagg actactcctt tattttgata atcagcaaga agaaacgatt
     9241 gcttttcgaa cagatatcga tgcactgcca gtcaaagaag aaacgaatct ctcttttgct
     9301 tctaagcatg aaggttatat gcatgcatgt ggtcatgacg gacatatggc gatgctttta
     9361 gggttgactg actttataac taaacacatt gaggatctta tttataatgt agtactgatc
     9421 tatcagccat cagaagaaat ttccggtggc gcacaaagcg ttattcagtc aggattgttg
     9481 gataaatatg aagtacaagc aatatttggc tttcatctat ggcctggact agctaaggga
     9541 gaagttttct ctcgaccagg acctttgatg gcacaaagca gtgaaacaga cattatcatt
     9601 aaaggccagt ctgcacatat tgcttctagt gaaaaaggaa tcgatagtct ggaggcagct
     9661 gtacgatttt tgaagaatgt ttatgatttt gaccggcaat taccggcaga gtggaatcat
     9721 ctaatgaaat ttggccaaat ccaaggtgga acgatccgaa atgttttagc tggagaagtt
     9781 attgtatccg gaagtatccg ttcttacagt tcagatatgc aaaattattt gaagagggga
     9841 ttagaccatt tagcagaaca gtttcactct acagctacta tttcttttcg ctataatgac
     9901 ggctatccag ctgtaatcaa ccatagaggc ttatatgcag cactgttaga ttcaaacaaa
     9961 ctacatgaat tagatgctcc agttcttcaa gccgaagatt ttggcatata tacgcaacaa
    10021 tatccatgtg tatttttctt tttaggtgtc ggtgataccc ctgctttaca tgatccgacg
    10081 ttcgattttg atatggatgt tttagaaaat ggtgtagaat ggtacaaaac gattcttttt
    10141 acagaaaatc ttcttgtaaa aatcgatagc agttcaaaag aggagcgagt aaataatgat
    10201 gaatttatcc aatcgattta gtcctaggct ttctagaata gaagtatcaa aaatacgaca
    10261 attcgatcag caaatatcgt cgataccaga tgtgatcaaa ctcaccctgg gagaaccgga
    10321 ttttccaact cctgaacatg tcaagcaagc aggaatagca gcgattgaag aagacttttc
    10381 tcactatacc ggaatgagag gattagagga actgcgggaa gcggcttgta tatttcagca
    10441 acagcgttat ggattaactt atgatccgca aacggaagtt ttgacgacag ttggtgcaac
    10501 ggaagcgatt gcctctgcgt tgttatctgt tttggaagaa ggagacaagg ttcttattcc
    10561 agcgcctgct tattcaggat atcagccatt agtggaacta gctggtgctg aattgatccc
    10621 gatagatact tctgacacag ggtttgtctg tcagccggaa caatttgaac gagcatttga
    10681 acaatatgga tcagctgtca aagcagtcat tttaaactat cctaacaatc ctactgggac
    10741 gacattgtct gcaaaacaat tgaaagcaat cgctgaggtt ttaaaaaaat atccggtatt
    10801 tgttataagt gatgaagtat atgctgaatt gacttatagt ggaacgcata tgtccatagc
    10861 tacttatctt ccagaacaaa caatcgtgat aagcgggtta tccaagtcac atgcgatgac
    10921 tggttggcga gtgggcttta tttttgctca aaaagccctt attgacgaac taattaaagt
    10981 ccatcaatat ctggtgactg ccgctacaac gatgtcgcaa aaagctgcgg ctgaagcgct
    11041 aataaatggg gtgaaagata gcgagaatat gaaagagcaa tatcaaacac gaagagacta
    11101 tctggtccag caattggcgc ctttaggatt tgaagtgacc cagccaaacg gtgcatttta
    11161 cttgttttgc aaactcccag ctgccattca aactgactct tggcagtttt gtgtagaatt
    11221 agcggaacag gcaaaagttg cgtgtatccc aggaattgca tttggcccag aaggagaagg
    11281 ttatatccgg atttcttatg ctagcagcat ggaaaaatta catgaggcct gcaaacggat
    11341 caaagacttt ttaacagaac agtgattcat agattttatc aaaaaaacaa tcgttaattt
    11401 tgcggtatat tctgcaagga gacgattgtt ttttaaattt agttcaaatg aacgcgtttc
    11461 ttttgattca taactaatgt aatgttaagt ttaaattgtt tagaatgatt ttaaaagaat
    11521 attcaaggag gtttcatcaa tggaaaaaat caaagttgtc attgtaggtg catctcatgg
    11581 agggcatcaa tcgatcttgg aactattgac acgatacggt gaaaatgtag atatcacgct
    11641 atttgaagca ggagactata tttccttcat gtcatgtggt atggagctgt atttagaaga
    11701 caaggtaaca gatgtaaatg atgtacgtaa tttcagacca gaaaactttc cgcaatccaa
    11761 tgttcatatt ttaaataatc acgaagtaaa aacgatcgat gccgataaaa aaacagtaac
    11821 ggttactagc gcatcagata acgaaacaaa agaatatcct tatgacaaat tgattttgag
    11881 ttctggtgtc aaaccaaaca atctgccagt accaggagca gatctagaaa atgtctactt
    11941 gatgcgtgga tacaattggg caaccaaaat caaagaaaaa ttaaatgatc cggctattaa
    12001 aaaaatcgct gtcgtaggtg cgggttacat tgggatcgaa gcagcagaag caagccgaaa
    12061 agctgggaaa gaagtcgtat tgcttgatgt gatcgataga cctttaggca cttatttaga
    12121 tgctgagatg acagatattt tagaacaaca cctaaatgaa aacggtattg aagttttgac
    12181 aaatgcaaaa atcgaagctt ttactggtaa cggaaaagtt gaggcaatca aaacaagtga
    12241 aaaagaaatc ccagcagatc tagtcattca agctgccggt gtgaaggcaa atacagaatg
    12301 gttaaaaggg atcgtcgact tagatgaacg tggctggatc caaacaaatg aatacttgca
    12361 aacgaatctt ccagatgtgt atgcagtagg agatgctaca cttgcttatt ctatcccagc
    12421 acgcaaaaaa atgccgatcg ctttagcgac tgttgcacgt agagaagcac gttatgtagt
    12481 aaaacatctt tttgaagaag taccaaacaa accgttcggc ggagtcgtag gttcctctgc
    12541 attaagtgtc tttgactatc attttgcagc aagcggattg aatagcttta cagcagcaaa
    12601 agcaggagta gatgtacgag tgtcttatta tgaagatacg ttacgaccaa aatatgtacc
    12661 agaagaacaa ggaaatccaa aagtgtccat ccaattattc tcggatccaa tgacccatca
    12721 attgctagga ggagcagtat tatctaccta tgatgttaca ggtcaaggaa atgtattggc
    12781 actagcgatc caaaacaaac tgactttaga agaactagca gaagctgact tcttcttcca
    12841 gccaggcttt gaccgacaat ggagcatgtt gaatctagcc gctcaacaag cgttaggaga
    12901 agaaccattt gtagaataag aaaacgaaat atttgaaaag aggttgtgaa aaagctgcct
    12961 tgcatcaagt aataagaaca gccaagtaaa aacagcttct tatgttttta acttggctgg
    13021 acttattacc gcagatgcaa gcttttgaac accgtttatt ttcaaaaaag ggactgtgac
    13081 acgatttgtg taccgacccc caaaagttag accaaaaaat ctaacttttg gaggtcggta
    13141 ttttttatgg ttaaatatac ttttgaactt aaattaaaga tcgttcatga ttatttggat
    13201 ggcaaaggcg attggaccgt aaaaatccag atcaagagat tgaagcggaa atgcttaaaa
    13261 ttcgggagaa gcacaaggat tatggctgtt tacggatgac caatgaacta agaaaccgcg
    13321 gtttctctat caataaaaag aaagttcagc gcttgatcaa gaagctagga ataaaagtaa
    13381 cggcatacac gcgtaaatca cgtcgctaca attcttatcg aggaaaagt
//
LOCUS       DAZKLM010000074        13212 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3764
            SAMEA115706913-rid22582563.denovo.077, whole genome shotgun
            sequence.
ACCESSION   DAZKLM010000074 DAZKLM010000000
VERSION     DAZKLM010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706913
            Sequence Read Archive: ERR13196647
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13212)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13212)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287823.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287823.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,004
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,849
            CDSs (with protein)               :: 2,849
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 41 of 96
            Pseudo Genes (incomplete)         :: 52 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 15 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13212
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706913-rid22582563.denovo.077"
                     /isolate="VRE3764"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            147..1016
                     /locus_tag="ACG842_002045"
     CDS             147..1016
                     /locus_tag="ACG842_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFQ0248574.1"
                     /translation="MKDFQLGAATYLKSVAIRVKDRDKMIDFYKNVVGFDLKREENEL
                     AILGTEEAGSEMLLLEESPRANDHMGVIKKLNRFSLIIPSTEEMADILCRIRKMNYPI
                     KEALEDHGRMGILLVDPEENELEIYHEENKNDQREIPETMDQEALLRKSEEKFSKLST
                     GSYFEKIHLNVTNLTKERQFLNHVLGLKVHNEEEGIHVLNEGDFHVGLTEASGGAIDL
                     PTDEVIGLDFLKFMIDTESMEGLIAHLQQLSVDFFLDKKHTVLTIYDAIGIEWWFVSE
                     PKEKNKTDIRCDA"
     gene            1225..2250
                     /locus_tag="ACG842_002046"
     CDS             1225..2250
                     /locus_tag="ACG842_002046"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HFQ0248575.1"
                     /translation="MYPHTKQLILKKMEEGFYPGVAVAFIKENQVETFTAGFAQVVPE
                     KQLMTEDLLFDAASLTKVVATTSLVLRLLEDGKIELDQPLHKYLPEFENQTITLRHLL
                     THTSDIKTWIPNRDKLSQEELIAAYLKLEPGEQLGKEVKYTDAGTILLGLMLEEIYQE
                     TAVEIFQEQVLEPLGMMNSLFLPFPSKKIVPTEQLPSGEVLRGQTHDPKARVLAEHAG
                     NAGLFIDLKDSITFVQMYLNKGKIRNGRFLEEETIDSLLQDQTPKKNGYRSLGWDLKK
                     DVEDGHAILFHTGYTGTFFLIDIKTQEALIFLSNRVHPVDHRAEYLICRDEIVENFLK
                     EKTVLPK"
     gene            2289..3239
                     /gene="manA"
                     /locus_tag="ACG842_002047"
     CDS             2289..3239
                     /gene="manA"
                     /locus_tag="ACG842_002047"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HFQ0248576.1"
                     /translation="MQEPMFLKPVFQEKIWGGSRLRSVFGFDIPNDKIGEDWAISAHP
                     HGVSVVENGEFKGKRLDELWSEHKELFGHPSEPVFPLLIKILDAEDDLSVQVHPDDAY
                     GMKHEGELGKTECWYIIDAEPGAEIIYGHHAKTREELAEMIKDGRWDDLLKKVPVKKG
                     DFFYVPSGTIHAIGKGIMILETQQSSDTTYRVYDYDRKDAQGQTRELHIQQSIDVTTV
                     PAKTPELQIKEVRKGNSSIVTYLETEFFNVYEWDIKGITSFKKQAPYTLMTVIEGAGD
                     LVVDGKTYPLEKGTSCIIPNGVSEWAVQGELAIIATEPGK"
     gene            3333..5036
                     /locus_tag="ACG842_002048"
     CDS             3333..5036
                     /locus_tag="ACG842_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFQ0248577.1"
                     /translation="MEYKWKANNSSRVSMKQLRKAGHHLNEMIMPNLSLFIAWGILSL
                     ATKGVSGDLHTTLEEVCHWMIQLLLPILISYSGGKSVGGQKGAVAGAIASLGLIARSN
                     APQIVGAMIIGPLAGIVYEQFVSRFHVKFKAGYEMLVSNLLVGLTGSVICIIGIVFIE
                     PVLNSVHLLLASVVSWLVTMNLLPLVSLILEPLKVLFFNNAINHGVLTPLGIEFSQTV
                     GYSSLFLMEANPGPGLGILLSILCFAEKQEKVNASGALMIHAIGGIHEIYFPFVLLRP
                     YLFLAVMAGGASGTLIFQWLDTGLAAPVSPGSLLVIFANAPYPQLAGVSIGIAISTFV
                     TFICAAILLKLPNKEKENKTVKERGEILEPSIIHSVIFACDSGMGSSAMGAALLKKKL
                     AAEKIDLSVGYSSIYELNNQVNQLVIVQHELAGLAREKVPQSQIYVLDHFLETDILLS
                     RIKEAIQFPKREEKKEIADITEQKKSPANIVFLYKNNRRGSQTMGMTILSQLAKKAKK
                     QFVISKESIEKIKIQEETIYIAQRELAVTEQLEEKIPRLVLVEHWVATNEYEKLVKGE
                     I"
     gene            5038..7071
                     /locus_tag="ACG842_002049"
     CDS             5038..7071
                     /locus_tag="ACG842_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HFQ0248578.1"
                     /translation="MYLSPREKQLLAEFLSSPSPVSIQKMMNLLKVSKRTIYRELDNL
                     EVSLKSVNASLKKAARGSFVIEASEEVLEQLKENTDGKSNDLSTAKRQHAILAELLLT
                     TEPLSLAHFMESYRISNTTFYADIKQLEESIRQLPLEIIRNQGYEIEGPEKYRRLLTA
                     NVLELEINEYELFHSISFDASLNYFFRFVDPQHLSLARKVIGEELIQKKTNLSDRKLE
                     HLVLMLTLTMERVTKNHLLTNETYNSLANKELLQTAKRLFSRIASETKQLYPVNEIVF
                     FAGLLNDFTNSFDQNFFEETFDTQLAYLVKQLIQEVSEETNVHFYEDETLYKMLLTHL
                     SGVFSRAVLQEETLTNPILEKIMVQYEEVANAIRKAVERIFQEKKLSEEEIAYMVLHF
                     ANSLERSPKESNISIAGFSPSGLASTSMLELRLKRYFPFIQQIHFYRIADLKKIDLQE
                     EYDVVISTSILPGYGGKYILVSPLLLEDEVKQLKEAFSHIEKKKRQKITYNSNNEWMI
                     EDTYEETMHFMDQINQLLGRFFIQELDNPETISETIRSLFPYFPEGLVTDEEVVHRRL
                     LKRYEQAPIGIPHTNMGLFHTSSSLISKPIFCIFNLKHPLTIEGMDRQPVDLSRMLVM
                     LAPSPIQEKDGILLGKISGAIIMNDLNTEIFRSGNKEIVYQLLAKILIEEIKK"
     gene            7082..7522
                     /locus_tag="ACG842_002050"
     CDS             7082..7522
                     /locus_tag="ACG842_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFQ0248579.1"
                     /translation="MIQLPAEKILLDQSYQNWKEALTEIGQWMTQQGGIKPAYIQSMI
                     ERQEKASVYIGNFVALPHGTDKDEEVVEEGIYLVQVPDGVDFGTKEEPKIATLLFVVA
                     LKKEHQLAVLQDLAFFCSDIDQVMALSDAKTIEEVQMIFEHGHI"
     gene            complement(7722..8849)
                     /locus_tag="ACG842_002051"
     CDS             complement(7722..8849)
                     /locus_tag="ACG842_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0248580.1"
                     /translation="MSLLTFERNLLLKNKVNFLFPLLLVVLFAFPLFFDHKLAYTEFD
                     ELNHNYEEMQRLIETLKEDENEKEFVESLEKSNKLIEAILHAKNTGNVQQMVEATYHY
                     EKDILDRLISGQRQGIPIIEQQKRVELLRYMKEHQIQRYSIFDLPAHLPLANYYENIF
                     SGMISSFLILCITALFLSSIISYEKRKQVISLVNLLPDSMVKKHSIRFTIYYGAAMLS
                     LVMPFLIVSILVIIKNGLGDFRYPVGTIIGQEIRILPMYEYLFQSFLFLLLWVLFLST
                     ISFLLSALFEHSLVNLLGTLLCLFLAEYRLFSSIGWIESISHYLPTSYVDFQNVIIGG
                     DIFSPLASEQVTFMNGILTLGIWSIVLLFIGMGTIYIKKSY"
     gene            complement(8852..9529)
                     /locus_tag="ACG842_002052"
     CDS             complement(8852..9529)
                     /locus_tag="ACG842_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFQ0248581.1"
                     /translation="MTLLIKNMNMKRGKKRVIRQLNLLVESGEVAALIAPNGFGKTTL
                     LNGIAQLLPTTYDQLSLHNISPTAYTDYRRSFFFLESTNQLSDYLSAVDYLTLIKYYW
                     QSKVECQTVFQRLKMTEYLTLPIGKMSLGMKQHVLLAAYLISDSSVMLFDEPLNGLDP
                     GSIDILNSLIRQWQKEGKTILLSSHAIANIQSICSKACFLKNGEIVTESSNMEEILAI
                     YRKLYEV"
     gene            9793..11559
                     /locus_tag="ACG842_002053"
     CDS             9793..11559
                     /locus_tag="ACG842_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol-specific transporter subunit IIBC"
                     /protein_id="HFQ0248582.1"
                     /translation="MERTIPKNNISLKARVQKLGSTLSSMVMPNIGALIAWGVLTALF
                     IPDGYLPNEALATMVSPMLTYLIPLLIGYTGGKVIAGDRGSVVGAIATMGVIVGTDIP
                     MMLGAMIMGPLGGYAIKKFDQLFQKRIKSGFEMLVNNFSAGLIGFGLALLGFSAIGPV
                     VDALTQAMAKGVEIILSAHLIPLTSIFIEPAKILFLNNAINHGILTPLGTEQVAEAGK
                     SILFLLEANPGPGLGVLLAFTLFGKGAAKSSAPGAMIIHFLGGIHEIYFPYVMMKPLL
                     FLSVIGGGMSGSFIFQLMDAGLRAPASPGSIIAILAMTPFNSYLPVILGVIVAAAVSF
                     GISAVILKADAKDTNEDFEKSVQETKAAKQASKGAQGIPQGATFSGVQKIIFACDAGM
                     GSSAMGASILRKKVQEADLPQTVTNQAISQLTDDSNTLIVTQVELQERAKQKAPSARF
                     VSVENFLNSPRYDEIIQELSSKQKTSVSEIENEPAEEFLSANIKQIKEILLVHDDRSG
                     SATMGMTVLKDILDKNHLTIPLRKVNIQDLKDEPDSLIVAKESLAEKVQMAAPQSIHL
                     SVNSMVNPQKYESIAQYLKQTA"
     gene            11574..12011
                     /locus_tag="ACG842_002054"
     CDS             11574..12011
                     /locus_tag="ACG842_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFQ0248583.1"
                     /translation="MQSLPFDMIELNRSFKTKEEAIRYCGQMLVEAGGVDEEYIDAMV
                     DRDKMLSVYMGNFIAIPHGTDEAKKYVKKSGICVVQVPDGVDFGTEEEEKIATVLFGI
                     AGVGDEHLQLVQQIALYCSDMDNVVQLADALSKEEVTENLAIA"
     gene            12024..13175
                     /locus_tag="ACG842_002055"
     CDS             12024..13175
                     /locus_tag="ACG842_002055"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HFQ0248584.1"
                     /translation="MRAVHFGAGNIGRGFIGEVLAANGFEITFVDVNHTIIDALNERK
                     EYEIELADESKEKITVKNVKGINNQTNPEKVIQEIAEADLVTTAIGPNILPFIAELIA
                     KGIQEREKEGSTTSLDIIACENMIGGSAFLEKEVYKYLPETSFTDKYIGFPNAAVDRI
                     VPLQHHEDPLFVQVEPFKEWVIDDSQRKNKKIQLKGVLYVDDLEPYIERKLFSVNTGH
                     ATVAYTGALLGYQTIDEAMQDALVVAQLKSVLQETGSLLIAKWGFDAEQHHAYIEKII
                     HRFQNKNISDAITRVARTPLRKLGYQERFTRPVRELQEHNLTCPHLTATMGIIFNYYD
                     PEDEQSRQLHEMKIHENLEQLIQEVTGINDPKTIGNIKQNVNRYAKQVA"
BASE COUNT         4489 a         2293 c         2731 g         3699 t
ORIGIN      
        1 tttataagag tttttactca ttaatttaca aaaaacaagc aaaatgcgaa ttttctcaca
       61 ttttttttaa caaaatcaaa gttattgctt gaaagaagaa gaatccatcg ttaaaataaa
      121 gcttgacgta gaggggagca agaaatatga aagattttca attgggggct gccacctatt
      181 taaaaagtgt agccattcgg gtgaaagacc gagataaaat gattgatttt tacaagaacg
      241 ttgttggctt tgatttgaaa agagaagaaa acgaattagc cattttagga acagaagaag
      301 ctggaagtga aatgttgctg ctggaagaaa gtcctagagc gaatgatcac atgggggtga
      361 tcaaaaagct gaaccgtttt tcattgatta tccctagcac agaagaaatg gcagatatcc
      421 tttgtcgtat ccgtaaaatg aattatccaa tcaaagaagc tttagaagat catggacgta
      481 tggggatttt gctcgtagac ccagaagaga atgaattgga aatttaccat gaagagaata
      541 agaacgacca aagagaaatt ccagaaacaa tggatcaaga agcattatta agaaaatctg
      601 aagaaaaatt cagtaaacta tcaacaggtt cttattttga aaaaatccat ttaaatgtta
      661 ctaacttaac aaaagaacgc caatttctta atcatgtact tggtttgaag gtacataatg
      721 aagaagaggg catccatgta ttgaatgaag gagatttcca tgttggtttg acagaagcta
      781 gcggaggcgc aattgatttg cctacagatg aagtgattgg acttgatttt ctgaaattca
      841 tgatcgatac tgaaagcatg gaaggactga ttgctcactt gcagcaacta tcagttgatt
      901 tcttcttaga caaaaagcac acagtactca caatctatga tgctatagga atcgaatggt
      961 ggttcgtcag tgagccaaaa gaaaagaata aaactgacat tcgatgcgac gcttaacttt
     1021 ttctttctta acgattgtcg catgtctcgt cattttatga acggtcatac tatcgcttat
     1081 tctagagata gaaagagaaa atagtatacc gggggaaact gcgcggtttt tcgcgcagtt
     1141 tttttgtgta aaaaaggttg attcctcata gattaagctt taagcttgga ctgtaagaat
     1201 ctgatagaaa agcaggagaa aaagatgtat ccacacacga aacagcttat tttgaaaaag
     1261 atggaagaag gattttatcc aggtgtagca gtcgccttca taaaagaaaa tcaagtagaa
     1321 acatttacag ccggctttgc ccaagtcgtt cctgaaaagc agctgatgac agaagatctg
     1381 ttgtttgatg cagcctcact gacgaaagtc gttgcaacca cttcattagt tttacgtcta
     1441 ttagaagatg gaaagataga actcgatcaa ccgcttcata aatatcttcc tgaatttgaa
     1501 aatcagacaa tcactttgcg tcatttactg acacatactt ctgatatcaa aacatggata
     1561 ccaaacagag ataagctatc tcaggaagaa ttgatcgccg cttatttgaa gctagaacca
     1621 ggtgagcaac taggaaaaga agtaaaatat acagatgccg gtacgattct tcttgggttg
     1681 atgctagagg aaatttacca agaaactgca gtggaaatct ttcaagaaca agtattggaa
     1741 ccattaggaa tgatgaacag cttatttctt ccttttcctt ccaaaaaaat cgttcccaca
     1801 gaacaactgc cttcgggtga ggttttgcgt ggacaaactc atgatccgaa agcaagagtt
     1861 ttggctgaac atgcaggtaa cgcgggtctc tttattgatt tgaaagacag cattactttt
     1921 gtccaaatgt atttgaacaa gggaaaaatc cgtaatggtc gttttttaga agaagagacg
     1981 attgattctt tactgcaaga tcaaacaccg aaaaaaaatg gctatcgttc tttaggatgg
     2041 gacttaaaaa aggatgtaga agacggtcat gcgattcttt ttcatactgg ttatacaggt
     2101 acgtttttct tgattgatat aaaaacacaa gaagcgttga tatttttgtc aaatcgcgta
     2161 catcctgtcg atcatcgtgc ggagtatctc atatgtcgag atgaaattgt tgaaaacttt
     2221 ttgaaagaga aaacggtatt accgaaataa tgctataata gctatgaaaa atgagaggag
     2281 tttgaattat gcaggaacct atgtttttaa aaccagtttt tcaagaaaaa atttggggag
     2341 gaagtcgttt acgttcagtc tttggttttg atatccctaa cgataaaatc ggagaagatt
     2401 gggcgatcag tgcgcatcct catggtgtca gtgtagtaga aaatggcgaa ttcaaaggga
     2461 aaagattaga tgaactatgg tcagaacaca aggaattgtt tggccatcct tcagaaccag
     2521 tgtttccatt attgatcaag atcttagacg cagaagatga tctatctgta caagtacatc
     2581 cagatgatgc ctatgggatg aaacatgaag gtgaattagg aaaaactgaa tgctggtata
     2641 tcatcgatgc tgaacctggt gcagaaatca tctatggtca tcatgcaaag acaagagaag
     2701 aactagctga gatgatcaaa gatgggcgct gggatgacct tttgaagaaa gtaccagtta
     2761 aaaaaggtga tttcttttat gtaccaagcg gaacgatcca tgctattggt aaaggaatca
     2821 tgattttaga aacacagcaa agttctgaca cgacttaccg cgtgtatgac tacgacagaa
     2881 aagatgcgca aggacaaaca agagagctgc atatccaaca atcgatcgat gtaacaactg
     2941 tccctgcgaa aacaccagaa ttacaaatca aagaagtccg aaaagggaat tcatcgatcg
     3001 tcacttattt agaaactgag tttttcaatg tatatgagtg ggatattaaa ggaatcacat
     3061 ctttcaaaaa acaagcccct tatacattga tgacggtaat cgaaggagca ggagaccttg
     3121 tcgttgacgg aaaaacatat ccattagaaa aaggaacaag ctgtattatt ccaaatggcg
     3181 tttctgagtg ggcggtccaa ggtgaactag caatcattgc gactgaacca ggcaaatagt
     3241 tcccagatag ctgaaagcag cagacggttt ttcgtttgct gctttttcta cataaaatcc
     3301 gtccatcaat aaatagaagt atggtgaaaa agatggaata caaatggaaa gcaaacaaca
     3361 gcagtcgcgt gtctatgaaa caactgagaa aagctggaca tcacttaaat gaaatgatca
     3421 tgccaaatct aagtctgttt atcgcttggg gaatcttgtc attagcaaca aaaggtgtgt
     3481 ctggcgattt gcacaccaca ttagaagagg tatgccattg gatgatccag ctgcttttac
     3541 ccatcttgat cagttattca ggcgggaaat cggtaggggg acaaaaaggg gcagtcgccg
     3601 gtgcaattgc ttctttaggg ttgatcgctc gttccaatgc tccacaaatc gtaggagcga
     3661 tgattatcgg gccgcttgcc ggcatcgtat atgaacaatt tgtttctaga ttccacgtga
     3721 aattcaaggc tggatatgaa atgcttgtca gcaacctact agtaggactg actggtagcg
     3781 ttatttgtat aattggaatc gtttttattg aaccagtttt aaatagtgtt cacttgttgc
     3841 tcgcctccgt tgtgtcttgg ctagtcacaa tgaatctgtt accacttgtc agtctgatct
     3901 tagaacctct aaaagtacta tttttcaata atgcaatcaa tcatggtgta ttgacacctt
     3961 taggaatcga attctcacaa acagtcgggt actcttcctt atttttgatg gaggcaaatc
     4021 ctggacctgg tttagggatc ttactttcta tcctttgttt tgcagaaaaa caggaaaaag
     4081 taaatgcgag tggagcattg atgatccatg cgattggcgg gattcacgaa atctattttc
     4141 catttgtctt gctgcgaccg tatctatttt tagctgtaat ggctggtggg gcaagcggca
     4201 cgttgatatt tcaatggtta gatacgggat tagctgcacc tgtatcgcct gggtcactac
     4261 tcgtgatttt tgccaatgcg ccttatccac aactagctgg ggtaagtata gggatagcaa
     4321 tcagtacatt tgtgaccttc atttgtgcag cgatactatt gaaattacca aataaagaaa
     4381 aagagaacaa aacggtcaaa gaaaggggag aaatcttgga accatctatc attcattccg
     4441 tcatttttgc ttgcgactca ggtatgggat caagtgctat gggagccgcc ctgctgaaaa
     4501 agaaattagc agcagaaaaa atcgatcttt ctgtcggata cagctctatc tatgaattga
     4561 ataaccaagt aaatcagctg gttatcgtgc aacatgaact agcgggtctt gcaagagaaa
     4621 aagtacctca aagtcaaatc tatgtactgg atcacttttt ggaaacagat atcttgctgt
     4681 cacgaataaa agaagcgatc caatttccaa agcgggaaga aaaaaaagag atagcagata
     4741 tcacagaaca aaaaaagtcg cctgctaata ttgtattttt gtataaaaac aacagacgcg
     4801 gctcacaaac tatgggcatg acgattttaa gtcaattagc aaaaaaagcg aaaaaacagt
     4861 ttgttatctc aaaagaatca atagaaaaaa tcaaaatcca agaagaaacg atttatatcg
     4921 cacaaagaga actagctgta actgaacagc tagaagaaaa gatccctcgg ctagttctag
     4981 ttgaacattg ggtagctacg aatgaatacg aaaaactcgt gaagggagaa atataagatg
     5041 tatttatcgc ccagagaaaa acaattatta gcagaatttc tcagtagccc gtcacctgta
     5101 tctatccaaa aaatgatgaa tttattgaag gtcagtaaac ggactattta ccgcgaatta
     5161 gataatcttg aagtctcatt gaaatcggta aatgcttctt tgaaaaaagc agctagaggc
     5221 tcatttgtca ttgaggcatc agaagaagta ttagagcaat taaaagaaaa tacagatggg
     5281 aaaagtaatg acttatccac tgctaaacga caacacgcga ttttagccga gttgttactg
     5341 acgacagagc cgttgagtct tgctcatttc atggaatcat atcgcatcag taataccact
     5401 ttttatgcag atatcaaaca gttggaagaa agtattcgcc aactgccttt ggaaatcatt
     5461 cggaaccagg gatacgagat cgaaggaccg gaaaaatacc gccggcttct gacagcgaat
     5521 gtattagaat tagaaatcaa tgaatatgaa ctttttcata gtatttcttt cgatgcatca
     5581 ctgaattatt tttttcgatt cgttgatccg caacatctat ctcttgcacg caaagtaatc
     5641 ggggaagaat tgatccaaaa aaagaccaat ctaagtgata gaaagcttga acatttggtt
     5701 ttgatgctga cattgacgat ggagcgtgtg actaaaaatc atttactgac aaatgagaca
     5761 tataatagtc tggcaaacaa agaattacta cagacagcta aacgattgtt ttcacggatc
     5821 gcttctgaga caaaacaact ttatccagta aatgagatcg tcttttttgc aggattatta
     5881 aatgacttca cgaactcatt tgatcagaat ttctttgaag agacgttcga tacacagctt
     5941 gcttatttag taaaacaact gattcaagaa gtaagtgaag agacgaatgt ccatttttat
     6001 gaagatgaaa ctttgtacaa aatgctgttg actcatttat ctggtgtgtt ttcaagagcc
     6061 gttttacaag aagagacact gaccaatccg attttagaaa aaatcatggt tcaatacgag
     6121 gaagttgcga atgcaatccg taaagcagta gagcggattt ttcaagaaaa aaaattatcc
     6181 gaagaagaga tcgcttatat ggtccttcat tttgcgaatt cattggaacg aagtccgaaa
     6241 gaaagcaata tcagtattgc aggattttcg ccaagtggat tagcttctac tagcatgttg
     6301 gaactacgat tgaaaaggta ttttcccttc atccaacaaa ttcatttcta tcggattgct
     6361 gatttaaaaa aaatcgattt acaagaagaa tatgatgttg ttatttctac atctatttta
     6421 ccaggatacg gtggaaaata tattttagtc tccccgctac tgctagaaga tgaagtcaag
     6481 cagctaaaag aagctttttc gcatattgag aagaaaaaaa ggcaaaaaat tacgtataac
     6541 agcaataacg agtggatgat agaagatact tatgaggaaa caatgcattt catggatcaa
     6601 atcaatcaat tactcggtcg tttttttatc caagaactgg ataatcctga gacgatttcc
     6661 gaaacgattc gcagcttatt tccttatttt cctgaaggac tagtaacgga tgaagaggtg
     6721 gttcatcgcc ggctgcttaa gcgatatgaa caagcaccta tcggtattcc tcatacaaat
     6781 atggggttat tccatacatc cagctcattg atttctaaac cgatcttttg tattttcaat
     6841 ttgaaacatc cgttgaccat tgaagggatg gatagacagc cagtcgattt gtcccgtatg
     6901 ttagttatgc tggcgccttc gccaatccaa gaaaaagacg ggattctttt aggaaagatc
     6961 agtggagcaa tcatcatgaa tgacttgaac accgagatct tccgctcagg aaataaagaa
     7021 atcgtttatc agttgttagc gaaaatcctg attgaagaaa taaagaaatg aaggtgaaaa
     7081 gatgatacag ctaccagcag aaaaaatatt gctcgatcaa tcctatcaaa attggaaaga
     7141 agcgttgacc gagatcggcc agtggatgac ccaacaaggg ggaatcaaac cggcatatat
     7201 ccaatcaatg atcgaaagac aagaaaaagc gagtgtctat atcgggaatt tcgtagcctt
     7261 gccacatggg actgacaaag atgaagaagt agtagaagaa ggaatctatt tagtccaagt
     7321 gcctgatggt gtggactttg gaacaaaaga ggaaccaaaa atcgcgacac tcttatttgt
     7381 agtggcttta aaaaaagaac atcagttagc ggtgctccaa gatttagcct ttttctgttc
     7441 agacatcgac caagtaatgg cgctcagtga tgcgaaaacc attgaagaag tccaaatgat
     7501 ttttgaacat ggacatatat aaccaatgtt cacagtaaaa aaagctgcct tgcatcaagt
     7561 aataacttca accaagataa aaataagagg agctattttt gttgggaggt tcttattact
     7621 gcggatgcaa gcttttgaaa actatttatt ctagaaaacc gtttatttga aataggggac
     7681 ggtgacaagg tttttgtcac cgcccctatt atttttgtgt tttaatagct cttttttata
     7741 tagatagtgc ccatacctat gaataataaa acgatactcc atataccaag tgtcaagata
     7801 ccattcatga aagtaacctg ttctgaagct agtggcgaga aaatatctcc tcctatgatc
     7861 acattttgaa aatcaacata agaagttggc aagtaatgac tgattgattc tatccatcca
     7921 atcgaagaaa acaagcgata ctctgctaag aaaagacaaa gaagtgtacc taaaagattg
     7981 actagagaat gttcaaataa agcagataga agaaaactga ttgtacttaa aaagagcacc
     8041 cacaacaaca ggaacagaaa gctctggaaa agatactcat acatcggaag gattcgtatc
     8101 tcctgaccaa tgatcgttcc tacaggataa cggaagtctc ccaaaccatt tttgattatt
     8161 actagtatag agacgatcaa aaatggcatc acgagactta gcatagctgc accataataa
     8221 atggtaaaac ggatgctatg tttctttacc atactatccg gtaatagatt gactagagag
     8281 attacctgct ttcttttttc ataagagatg atggaagaaa gaaacaaggc ggttatacat
     8341 aaaatcaaaa aactagaaat cattcctgaa aaaatatttt cgtagtaatt tgctaaaggg
     8401 agatgcgctg gcaagtcaaa aatagaatat ctttggatct gatgttcttt catataacgg
     8461 agcaactcga cacgtttttg ttgttcaatg attggaattc cttgtctttg accagaaatc
     8521 agtcgatcca gtatatcttt ttcataatga taagttgctt cgaccatttg ctggacattc
     8581 cctgtgtttt ttgcatgtaa gattgcttcg atcagtttgt tgcttttctc taaactttcc
     8641 acaaattctt tctcattctc atcttctttc aacgtttcaa tcaaacgttg catctcttca
     8701 tagttatgat tcagttcatc gaattctgta taagcaagtt tatgatcaaa aaaaagtggg
     8761 aatgcaaaga gcacgaccaa caatagagga aataaaaagt ttactttgtt ttttagtaag
     8821 agattgcgtt caaatgtaag taaagacata gttacacctc atataattta cgataaatag
     8881 ctaaaatctc ttccatattt gaggattctg tgacgatttc tccatttttt agaaaacatg
     8941 ctttagaaca aatcgattgg atattcgcga tagcgtggga agaaagaaga atcgtcttcc
     9001 cctctttctg ccattgtctg atgaggctat tcaagatatc aatacttcct gggtctaatc
     9061 cgttcaacgg ttcatcgaat aacatgacag atgaatcact gatcaagtaa gcagcaagca
     9121 agacatgttg tttcattcct agagacattt ttccaatcgg caaagtaagg tactcagtca
     9181 tcttcaatcg ttggaaaaca gtttggcatt ctactttgct ctgccaatag tatttgatca
     9241 aagtcaaata atcaacagcc gatagatagt cggataactg attcgttgat tctagaaaaa
     9301 agaaagaccg gcgataatct gtataagcag taggagagat gttgtgaagg gacaattgat
     9361 cataagttgt tggcagcagt tgggcaatgc cattcagcaa agttgttttg ccaaatccgt
     9421 tcggggcaat caaagcagct acttctcctg attctactaa aagattcaat tgtcgaatca
     9481 cacgtttttt accacgtttc atgttcatgt ttttgatcag taatgtcata gataggcctc
     9541 caatataaaa tttaataaca taatagaatg gaaaacatct aaattaaatg ctaacaaaag
     9601 ttattgtttg catattataa aaaaattaaa ataacagttt tatttaaaat tgaagttttc
     9661 tgataccgaa atctttttat tttgctgaca tactcaaacc tttggcacaa agtttcgtgc
     9721 caaagtacga ctatttctct ttttagtaag cggatacaat tagagtatca aaaggagaga
     9781 gggagagaca aaatggaacg gacgattccc aaaaacaaca tttcgctaaa agcgagggta
     9841 caaaaattag gcagtacttt atcaagcatg gtcatgccaa atattggagc actgattgcc
     9901 tggggtgtat taacggctct atttatccca gatggttatt taccaaatga ggctttggct
     9961 accatggtaa gcccaatgct aacttatctg attcctttat tgatcggtta tactggcgga
    10021 aaagtaattg ctggtgaccg cggatcagtt gttggggcaa ttgcaacgat gggtgtgatt
    10081 gtcggaacag atattccaat gatgctaggc gcgatgatca tgggacctct tggtggatat
    10141 gccatcaaga aatttgatca gctattccaa aaacggatca aatccggttt tgaaatgcta
    10201 gtcaataatt tttcagcagg tcttatcggt tttggattag cgttattagg tttttctgcg
    10261 attggtccag ttgtcgatgc attgactcaa gcaatggcaa aaggtgtaga gatcatttta
    10321 tctgcccatt tgattccatt gacaagtatt tttattgaac cagcaaaaat cttatttttg
    10381 aataatgcaa tcaatcatgg gattttgaca ccattgggaa ctgaacaagt agcagaggct
    10441 ggaaaatcta ttctattctt gcttgaagca aatcctggtc caggacttgg cgtattactt
    10501 gcttttactt tgtttggcaa aggagcagca aaatcttcag caccaggagc catgatcatt
    10561 cactttttag gcgggatcca tgaaatctat tttccttacg ttatgatgaa accgttatta
    10621 ttcttatcag ttataggcgg aggaatgtca ggaagtttca ttttccaatt gatggatgct
    10681 ggactaagag cgccagcttc tccagggtcg atcattgcga ttctagcgat gacaccgttc
    10741 aattcttacc ttcctgttat tttgggcgtg attgttgccg cagctgtttc gtttggtatt
    10801 tctgcagtaa ttttgaaagc ggatgcgaaa gatacaaatg aagacttcga aaaatctgtt
    10861 caagagacaa aggcagctaa gcaagcttcc aaaggtgccc aaggtattcc tcaaggagct
    10921 acattctctg gcgtacaaaa aatcattttt gcttgtgatg caggaatggg atcaagtgcg
    10981 atgggcgctt cgatcttgag aaaaaaagta caggaagcag acttgcctca aacggtgacc
    11041 aatcaagcaa tcagccaact gacagatgac tcgaatacat tgatcgttac tcaggtcgaa
    11101 ctacaagaaa gagcaaaaca aaaagcaccg agcgcgcgtt ttgtctcagt agaaaacttt
    11161 ttgaattcac ctcgatatga tgaaatcatt caagaattaa gcagcaagca gaaaacatca
    11221 gtttctgaaa ttgagaatga gccagcagaa gagtttcttt cagcaaacat caaacaaatc
    11281 aaagagattc tacttgtcca cgatgatcgt tccggatcag caacaatggg gatgaccgtg
    11341 ctcaaagata tattagacaa gaaccattta actatcccgt taagaaaagt caatatccaa
    11401 gatttaaaag atgaacctga ttcactgatc gtagccaaag aaagtttagc ggaaaaagta
    11461 cagatggctg cacctcaatc cattcattta tcagtcaaca gcatggtcaa tccgcaaaaa
    11521 tacgaatcga ttgcacaata tttgaaacag acagcttaag ggaggaaaac agcatgcaaa
    11581 gtttgccatt cgacatgatc gagctcaatc gatcttttaa aaccaaagaa gaagcgatcc
    11641 gctattgcgg tcagatgctt gtagaagcag ggggcgttga tgaggaatat atcgatgcca
    11701 tggtagatag agacaaaatg ttatctgttt atatggggaa ttttatcgcg attcctcatg
    11761 gaacagatga agcaaaaaaa tatgtgaaaa aatccggcat ctgtgtcgtt caagtaccag
    11821 atggtgtaga ttttggtacc gaagaagaag aaaagattgc gactgttttg ttcggtattg
    11881 caggagtcgg agatgagcat ttgcaacttg tacagcaaat cgcactttat tgcagtgata
    11941 tggacaatgt tgtccagcta gcagacgcgc tcagcaaaga agaagtaacg gaaaatctag
    12001 caattgctta aggagagaga aaaatgagag cagtacattt tggtgcagga aatatcggtc
    12061 gaggatttat tggagaggtt ttggcagcta atggatttga gattactttc gtagatgtga
    12121 atcacacgat cattgatgct ttaaatgaaa gaaaagaata cgaaatcgaa ttagcagatg
    12181 aatcaaaaga aaaaatcact gtaaaaaatg tcaaaggaat caacaatcaa acaaatccag
    12241 aaaaagtaat ccaagaaatc gctgaagcag atttagttac taccgccatt ggcccgaata
    12301 tcttaccttt tattgctgag ttgattgcaa aaggtatcca agaaagagaa aaggaaggaa
    12361 gcacaacatc tttagatatc attgcctgtg aaaatatgat tggcggttca gcatttctag
    12421 aaaaagaagt gtataaatat cttccggaaa catcatttac tgacaaatat atcggctttc
    12481 caaatgcagc agtcgatcga atcgtcccat tacaacacca tgaagatcct ttattcgtcc
    12541 aagtggaacc tttcaaagaa tgggtcattg acgatagcca aagaaaaaat aaaaaaatcc
    12601 aattaaaagg ggttttatat gtagatgatc tagaaccata tatcgaacgc aaactattta
    12661 gtgtcaatac tggtcatgcg actgtcgctt atacaggtgc cttgttaggt taccagacca
    12721 ttgatgaagc aatgcaagat gcgttagttg tggcacaatt aaaatcagtt ttgcaggaaa
    12781 caggaagttt gctgattgcc aaatgggggt ttgatgcaga gcagcatcat gcgtatattg
    12841 aaaaaatcat tcatcgcttc caaaacaaaa atatctctga tgcgatcaca cgtgtcgctc
    12901 ggacaccatt acgtaagtta ggttatcaag aaagatttac acgtccagtg agagaactac
    12961 aagaacataa cttgacttgc ccacatttga cggcaacaat ggggattatt tttaattact
    13021 atgatcctga agatgaacaa agccgtcaat tgcatgaaat gaagatccat gaaaacttgg
    13081 aacaattgat tcaagaagtc acaggaatca atgatccgaa aacaatcggg aatatcaagc
    13141 aaaacgtgaa ccgttatgcc aagcaagtcg cttaaaatag gatacgaaaa aggggaatgt
    13201 gacgaaattt tt
//
LOCUS       DAZKLM010000075        13193 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3764
            SAMEA115706913-rid22582563.denovo.078, whole genome shotgun
            sequence.
ACCESSION   DAZKLM010000075 DAZKLM010000000
VERSION     DAZKLM010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706913
            Sequence Read Archive: ERR13196647
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13193)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13193)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287823.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287823.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,004
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,849
            CDSs (with protein)               :: 2,849
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 41 of 96
            Pseudo Genes (incomplete)         :: 52 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 15 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13193
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706913-rid22582563.denovo.078"
                     /isolate="VRE3764"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            158..1897
                     /gene="yfcC"
                     /locus_tag="ACG842_002056"
     CDS             158..1897
                     /gene="yfcC"
                     /locus_tag="ACG842_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HFQ0248585.1"
                     /translation="MKKTNSSGKPKRDFTKLSTPHTYVIIFGVVIFAWILTFVVPAGK
                     FSTQDIEYKDANGETSTRTVLRQDSFRYAYELDKSYVFDQLEELQDHPAEREKLDVPE
                     KGLEKVIADGEKNLTQEKLDEISLTDDVLYDQYGENIYDTSKKLHKTAKIWGTDDFGG
                     FGFLNFVFEGLVSGDKYGSAVGIAALILVVGGAFGIIMRTGAIDAGIYAFISKTKGLE
                     RLALPLLFFAFSFGGATFGMAEEVIPFSMVMVPFVIALGYDSIVAVTVTYVASQVGNA
                     TSWMSPFSVAVAQGIAGIPVLSGATFRLIMWVVVTALAAGYMMIYAEKIRKKPESSLT
                     YKSDDYFRSHIKKTSDENKKFMLGHKLILLEMLVVLVWIVWGVTQKGYYIPEIASQFF
                     VMGLVAGIIAVLFKLDGMKVNDIASSFQSGAADLAGTAIVVGMAKGILLVLGGSDASV
                     PSALNTILHGIGTALTGVPAVIGAWAMYIFQSLFNLVVTSNSGQAALTMPIMAPLADL
                     VGVSRQVAVLAYQLGSGFMDAFTPVSASLIGVLGVARIEWAKWARFQIKMQGFLFVLG
                     TIFIMIAIAIGLQ"
     gene            2054..3238
                     /gene="iadA"
                     /locus_tag="ACG842_002057"
     CDS             2054..3238
                     /gene="iadA"
                     /locus_tag="ACG842_002057"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008798 - beta-aspartyl-peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HFQ0248586.1"
                     /translation="MKIIRQIEVFAPDYLGKMDVLIAGGKILAVEEQLEGGYEGVEVE
                     ELSGEGKVLTPGFIDCHFHILGGGGEGGYQNRTPEVTLSQLTTAGVTTVVGCLGTDGE
                     GRDMTALISKAKGLEAEGISTYVYEGSYRLPVKTVTDSIIKDFLTIDKIIGIGEIAVS
                     DHRSSQPSFEEFARAVADARVGGMLSGKAGIINVHLGGGKRKLELLTRIVEETEIPIT
                     QFLPTHANRTPELFEACVAFAKRGGTIDFTASEDPDFWEKTDGEVRFSKALKRLIEED
                     VSLDNFTMTSDGQGSLPYFDENNHFLGLGVGSAKALLVGIKEAVQKESIPLEIALRAI
                     TSNPARILKLDKKGKIEIGADADLCILDKETLDIDTVIAKGEIMVQEKEVKVWGTFEK
                     SF"
     gene            3349..3552
                     /locus_tag="ACG842_002058"
     CDS             3349..3552
                     /locus_tag="ACG842_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HFQ0248587.1"
                     /translation="MADLKGRSKDMKDKVVGEGKEAYGKATDDKSKETEGKAQSLGAD
                     IKEKARNLGDEVKDKFSRKDEKK"
     gene            complement(3653..4315)
                     /locus_tag="ACG842_002059"
     CDS             complement(3653..4315)
                     /locus_tag="ACG842_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0248588.1"
                     /translation="MLRTEQLGFWYQDPQDALYKDVELNFEAGKMYAILGASGSGKTT
                     FLSLISGLDAPKEGKVYYQDRSLNKIGLRSYRKNDVSIIFQAYNLLPYLSALDNVIAA
                     MEITKTKQENYRQVALDRLARVGIDEILAKKQVTKLSGGQQQRVAIVRAICCEHQLIV
                     ADEPTGNLDETTSQDIVKLFQEIAHEQNRCIIIVTHEIEVAKSCDEVFELKNKKFQKI
                     EV"
     gene            complement(4328..5830)
                     /locus_tag="ACG842_002060"
     CDS             complement(4328..5830)
                     /locus_tag="ACG842_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0248589.1"
                     /translation="MNFMKRAWKSTKVKWGRSLLLFAVFTAILIFVLAGLTIRSAAEE
                     AANQAQKEVGATVTLSANREASFKKQEQTSSSGSTTEERPDPGSFSLTPVSLTDAEKI
                     AELDNVKSYSFESSASAMAKDGITAITSSDSSEDSSETTDAGGEPAGGMGGNPQMTQA
                     DFQISGVSATAQNSNFSDGTAKITDGEGIDDSDKGTNNVVIDSTLAETNDLAVGDTFT
                     ITSTEDEDTTYEMTIKGIYESRETSGSMGMKFNFMNPSNTLYTSYTFANELNGTSDDN
                     TIDSAIYTLSDPDKMDEFLKEAEKLIDTDTFSLQSNDSMYQSMLEPLNNVASFSKNVV
                     LLVAVAGIIILTLIIMITIRERRHELGVLLSLGESRSKIILQLFTEVAICMILALGVA
                     SLSGNVVANAVGQQLLDQQTETTTQNQQAPGGGQMPGNRQEQGGQRDGQQGTTNNPFA
                     VSDQVSELEINVKPAQLGLLAGVAFGISLFSVFLASIGILRLNPRKILLN"
     gene            6378..8057
                     /locus_tag="ACG842_002061"
     CDS             6378..8057
                     /locus_tag="ACG842_002061"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HFQ0248590.1"
                     /translation="MSTIKIIPLGGVRENGKNMYVAEVGEDIFILDCGLKYPENELLG
                     IDVVIPDFTYLEENIDRVAGVFLTHGHADAIGALPYLLSKISVPVFGTELTIELAKLN
                     VSRNEAAKGFKDFHVVDEHTEIDFGETVVSFFRTTHTIPDSVGINLKTKEGSIVYTGD
                     FKFDQTAIPMYQTDYARLAEIGKEGVLALLSESANAENPQPVASEAQIADEVFDTIKY
                     WEGRIIVACVASNLQRVQQVLDAAEKAQRKVVLTGQDFERIIRTAMRLEKLQLPSEDL
                     LVTLKDMKNYDPEELIILETGRMGEPIKSLQKMANGTHRNVKIEEGDLVYITTTPSIA
                     METIVAKTEDIIYRAGGTVKLISENMRVSGHANPNDLQLMINLMKPTYFVPVQGEYRE
                     LAAHADLAHAVGMPYKNIYITGRGDVLEYKNKRMSVAGTTSAENVMIDGLGIGDIGNI
                     VLRDRKILSEDGIFVAVVTISRREKKIISKPQITSRGFVYVKASRDLMKESSNMIETI
                     VEKHLESNDFEWSKLKQDIRDQLGRYLFEQTKRRPVILPVIMEATQRRNKK"
     gene            8184..8663
                     /locus_tag="ACG842_002062"
     CDS             8184..8663
                     /locus_tag="ACG842_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HFQ0248591.1"
                     /translation="MMSQTKVERYLEEQKISYQPLDFSHVVEESFTEELQKRGIDPAL
                     ICKTLVVKGNKTGVIIALIPLNDHLDYKKTRQVTKDRKVGFPGMDFVLMHTGYPHGAN
                     TPIGIKLAHPDYCFLADSSLKEKEEVIVSSGEIGRSLRIKVADLEKIVHPIYADLIS"
     gene            8882..9562
                     /locus_tag="ACG842_002063"
     CDS             8882..9562
                     /locus_tag="ACG842_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFQ0248592.1"
                     /translation="MKITYHGHSCLTIEMENGQRVMIDPFITGNPLTDLTAEEVKTDW
                     LLITHGHSDHIGDMIPIAKENQATIISIVEIADYANSRGVDSFGMNIGGKHAFPFGVV
                     KFVHAQHSSSYEVDGIVQYMGEPSGIIIQAEGKTIYHAGDTAYFSDLGLLAEEFDIDV
                     AFLPIGDNYTMGPEDAAKAAQTIRAKKVVPIHYNTFPVIEQDPESFVELLEDSEGKIM
                     SVSETLEV"
     gene            9570..10901
                     /locus_tag="ACG842_002064"
     CDS             9570..10901
                     /locus_tag="ACG842_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DRTGG domain-containing protein"
                     /protein_id="HFQ0248593.1"
                     /translation="MATKHDLILEYIESLPVGNRISVRSIAKELNVSEGTAYRAIKDA
                     ENVGLVSTIQRVGTIRIERKLKKHIERLTFGEVVRIIEGDVLGGAAGLDKVLNKFVIG
                     AMTKHAMERYITPGSLMIVGNRAEVQKLALEDGAAVLITGGFNTTAEIAKLADDLEMP
                     ILRTSYDTFTVATMINRALSDQLIKKDIMLVSDIYMPAEKTHYLQQTDTISDYQRLSE
                     ETQHSRFPVVNRHHRLMGIVTAKDVLGKSPNQIIDRVMTKEPISVKKTMSIASVSHQM
                     IWDGLEVMPVVSDDLTLEGLITRQDVMKAMQLVQRQPQIADTISDQISGNIHTIDTDR
                     EGNRLENPKFKFVVAPQMVNGVGTISFGVLSEIISDVAQKTMVMNQKRNILIEQVNLH
                     YLRLIQLESELDIRPRVLEIGRRSAKLDIEVFIENTIVSKAIVVCQVMERS"
     gene            10916..11878
                     /locus_tag="ACG842_002065"
     CDS             10916..11878
                     /locus_tag="ACG842_002065"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HFQ0248594.1"
                     /translation="MTVQQEIVDTIKKYDKIIIHRHLRPDPDALGSQVGLAELLRESF
                     PEKEIRQVGETIEGLRFLAEMQEVEDVFYQGALVIVTDTANSPRISDQRYQLAEKLIK
                     IDHHPNDDPYGDLVWVNTKASSCSEMIAEFALMFPESLKMNVKAARLLYAGIVGDTGR
                     FLYPSTTSRTLEIASILRNYPFDASALNREIEQMPMKVAKLSGYLYQNIQVDENGAGR
                     IILPQEILDKYGIDDSETASIVSLPGVIDEVLAWGIFVQQPSGYYRVRLRSKGPIINE
                     LAKRHHGGGHPLASGANAKDLAEVEEIYKEIQDICQSYKSRKTE"
     gene            11989..12327
                     /locus_tag="ACG842_002066"
     CDS             11989..12327
                     /locus_tag="ACG842_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein YjdM"
                     /protein_id="HFQ0248595.1"
                     /translation="MTLPNCPECGSEYTYEDRGLLICPECGNEWNKEEQETVIEDGLV
                     VLDANGNRLLEGDSVTVIKDLKVKGASGAIKQGTKVKNIRLVEGDHNIDCKVEGFGPM
                     KLKSEFVKKN"
     gene            12406..13113
                     /locus_tag="ACG842_002067"
     CDS             12406..13113
                     /locus_tag="ACG842_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HFQ0248596.1"
                     /translation="MYGYGMYGFLDPTFLLVILGLVISGIASAYVNSTFRKYDQVRSK
                     NNVTGTQAAQYILQSQQINNVGVQQIAGDLTDNYNSGNKMLSLSQATAQSTSVAAIGV
                     AAHECGHAVQDASGYVPLKIRAALVPAANFGSMISFPLIMVGVLLSWNSTLINIGIFA
                     FSLALLFQLVTLPVEFNASRRAIQILSEGGLLTEEEVPMARHVLFAAALTYVAAALST
                     FLQLLRLILLFGGNRRD"
BASE COUNT         4208 a         2324 c         2887 g         3774 t
ORIGIN      
        1 aaaaaaataa tcatacatag gagaaaaaaa acaaaataga aaaaagtgat tttctaataa
       61 attattcatg tggaaatgtc atttttactg tataattcat ttgattatcc attttttatt
      121 catcaaaatt actgaaaaaa gaaaggaaga aataaatttg aagaaaacaa attcttcagg
      181 aaaaccgaaa cgcgacttca ctaaattatc gacaccccat acgtatgtca ttatttttgg
      241 tgtagtcatc tttgcatgga tactgacttt tgtcgttcca gcaggaaagt tcagcacgca
      301 agatattgaa tacaaagatg caaatggcga aacaagtact cgtactgttt tacgtcaaga
      361 ttccttccgt tatgcttacg agctggataa gtcgtatgtg tttgatcaat tagaagaact
      421 gcaagatcat ccagcagaac gtgaaaaact ggatgttccg gaaaaaggat tggaaaaagt
      481 cattgctgac ggagaaaaaa atcttacgca ggaaaaacta gatgaaatct ccttgacaga
      541 tgatgtgctt tacgatcagt acggcgaaaa tatctatgat acttctaaaa aattgcataa
      601 aacagccaaa atatggggaa cagatgattt tggcggcttt ggtttcttaa attttgtttt
      661 tgaaggacta gtatctggag ataagtatgg atctgctgta gggatcgctg cattgatcct
      721 tgttgttgga ggtgcttttg gtattattat gcgtaccgga gcgatcgacg caggtatcta
      781 tgcgtttatc agtaaaacta agggactgga gcgtttagct ttaccgctat tattctttgc
      841 tttttcattc ggtggcgcta cattcgggat ggcagaagag gtaattccat tctcaatggt
      901 gatggttcca tttgtcattg cgttaggata tgactcgatc gtagcagtaa ccgtgactta
      961 tgtcgcatct caagtcggaa atgcgacctc ttggatgagc ccgtttagcg tagcggtggc
     1021 tcaagggatt gctggaatcc ccgtgttgtc tggtgcgaca ttccgattga ttatgtgggt
     1081 agttgttact gcattagccg ctggttatat gatgatatat gcagaaaaaa tccgtaaaaa
     1141 accagaaagt tcgttgactt acaaatcaga tgattatttc cgttctcata tcaaaaagac
     1201 atctgatgaa aataaaaaat tcatgcttgg gcataaattg atcttattag agatgttagt
     1261 tgtccttgtc tggatcgttt ggggcgtgac tcaaaaaggc tattacattc cagaaatcgc
     1321 ttctcaattc tttgtgatgg gattagttgc tggaatcatt gctgttcttt tcaaattaga
     1381 tggaatgaaa gtcaatgaca tcgcttcttc tttccaatct ggtgcagctg atttagctgg
     1441 tacagcaatc gtagtaggta tggccaaagg gattcttctt gtcttgggag gatctgatgc
     1501 atctgttcct tcagctttga atacgatttt gcatgggatc gggacggcgt tgaccggtgt
     1561 tccagctgtc attggtgctt gggcgatgta catcttccaa agtttgttca acttggttgt
     1621 tacatcaaat tcaggacaag cagctttgac tatgccaatc atggctccac tggcagattt
     1681 agtaggtgta tccagacaag tagcagttct tgcttatcag cttggttcag gttttatgga
     1741 cgcttttaca cccgtatcag ccagcttgat tggtgtgcta ggtgttgcac ggatcgaatg
     1801 ggcaaaatgg gcaagattcc aaatcaagat gcaaggattc ctgtttgttt taggaactat
     1861 tttcatcatg attgccattg caataggtct gcagtagtac tggagacctg taaataaaaa
     1921 agtaatccaa acagtagata aggactccaa gaaccgtcat tttacggtaa aatcaaagga
     1981 gtaaagatcc tatcgtttgg attatttttt aagatcctat catcagttat acttaaagaa
     2041 agaggaaaaa tgtatgaaaa tcattcgaca aattgaagtg tttgcaccag attacttagg
     2101 aaaaatggat gtactgattg ccggtgggaa gattttggct gtagaagaac agctagaagg
     2161 cggctacgaa ggagtggaag tagaagaact ttcaggtgag ggaaaagtac taaccccggg
     2221 tttcattgac tgtcatttcc atattttagg tggaggagga gaaggcggtt atcaaaatcg
     2281 aacgccagaa gtcacattga gccaattgac tacagctggt gtaacgacag ttgtcggttg
     2341 tttgggaaca gacggagaag gtagagatat gactgcgttg atcagcaaag cgaaaggtct
     2401 ggaagcagaa ggaatctcca cttatgtata cgaaggttcg taccgattgc cggtaaaaac
     2461 agtaacagat tctatcatca aagacttttt gacaattgat aaaattatcg gaatcggaga
     2521 aatcgcagta tccgatcacc gttcttctca gccaagtttt gaagagttcg ctcgtgcagt
     2581 agcagatgct cgtgtaggag gcatgttatc cggaaaagct gggatcatca atgtccactt
     2641 aggcggagga aaaagaaagc tagagctatt gacacgaatc gtagaagaaa cagaaattcc
     2701 gatcacacaa tttttgccta ctcatgcaaa tcgcacacca gaattatttg aagcttgcgt
     2761 ggcatttgcc aaacgtggtg gcacgattga cttcactgct agcgaagatc ctgatttttg
     2821 ggagaagaca gatggtgaag ttcgcttcag caaagcactg aaacgattga tcgaggaaga
     2881 tgtttcttta gataatttca cgatgacctc agatggacaa ggaagcttgc cttattttga
     2941 tgaaaacaat catttcctag ggttaggagt aggaagtgcc aaggcattgc tagtagggat
     3001 caaagaagct gtgcaaaaag aaagtatccc tttggaaatc gccttacgag ctataaccag
     3061 taatccagca cgtatattga aattggataa aaaaggaaaa atcgaaattg gtgctgatgc
     3121 agatctgtgt atcttggata aagaaacgct tgatattgat acagttatcg ctaaaggaga
     3181 aataatggtc caagaaaaag aagtcaaagt atgggggact ttcgaaaaaa gtttttaatc
     3241 aacagctcta actatctttt ttagaaaacg agcgttctgt ttgtctgtat ctgttgttga
     3301 tgttaccctg aaggtagatc aataaacaaa ctaagaggag gaagcacaat ggcagattta
     3361 aaaggacgtt ctaaagatat gaaagataaa gtagtcggtg aaggaaaaga agcatacggc
     3421 aaagcaacag acgacaagag caaagaaaca gaagggaaag cacaatcact aggtgctgac
     3481 atcaaagaaa aagctcgaaa tttaggcgac gaagtcaaag acaagttttc tcgaaaagac
     3541 gaaaaaaaat aaaaaccact tattactaat tgaatcaagc aaataaaaaa tccgaaccat
     3601 atctgcgaga cagatatggt tcggattttt tatttgatca ttatcctgtt ttttaaactt
     3661 cgattttttg aaatttttta ttttttagtt caaagacttc atcacagctt tttgctactt
     3721 caatctcatg agtcacgatg ataatacagc gattttgttc atgggcgatt tcttggaaaa
     3781 gtttgacgat atcttgtgaa gttgtttcat ctagatttcc agtaggctca tccgctacga
     3841 ttagttgatg ttcacagcag atagcacgaa cgatagcgac tcgctgctgc tgacctcccg
     3901 aaagtttggt cacctgtttt tttgctaata tttcatcaat tcctacacga gccagtcgat
     3961 ctaaagcaac ttgtcgataa ttctcttgct tggtcttagt gatttccatt gctgctatga
     4021 cgttgtctag agccgataaa taaggaagca agttgtaagc ttggaaaata atcgagacat
     4081 catttttacg ataacttctt aacccgattt tgttcagtga acgatcttga tagtatacct
     4141 ttccttcttt tggtgcatct agtccagaaa taagcgaaag aaaagtcgtt tttccagaac
     4201 cacttgctcc taaaatagcg tacatttttc cagcttcgaa attcagttct acatctttat
     4261 aaagcgcatc ttgcggatct tgataccaaa atcccaattg ttctgtccgt aacatcaaat
     4321 tcctccttta gtttaatagg atttttcttg ggtttaatct taatatccct atagaagcta
     4381 aaaagacaga aaacagactg attccaaatg ctactccggc taatagtccc aattgtgcag
     4441 gtttgacatt gatttccaat tcgctgactt gatcagaaac agcaaatgga ttattcgtgg
     4501 ttccttgttg accatcgcgt tgaccgcctt gttcttgtct gttacctggc atttgtccac
     4561 cgccaggagc ttgctggttt tgtgtcgtgg tttctgtctg ttggtcaagt agctgctgtc
     4621 ccacggcatt cgcgacgacg tttccgctaa gcgaagcaac tcctaatgca agaatcatac
     4681 aaatagccac ttcggtaaac aattggagaa tgattttact tctggattct cctaaagata
     4741 gcaagacgcc taactcatgc cgacgctccc gaatggtaat cataatgatc agagtaagta
     4801 tgataatacc ggctacagcc actagtaaaa caacattttt agaaaaactt gccacgttgt
     4861 ttaaaggttc caacattgac tgatacatgg aatcatttga ttgtaagcta aatgtatctg
     4921 tgtcgatcag cttctcagct tctttgagaa actcatccat tttatctgga tcagatagag
     4981 tgtaaatagc agaatctatc gtgttatcgt cagatgtccc atttagttca tttgcaaaag
     5041 tgtaagaagt gtaaagtgta ttcgacggat tcatgaagtt gaatttcata cccatactgc
     5101 cgcttgtttc tctggattca taaattcctt tgatcgtcat ttcataagtg gtatcttcat
     5161 cttcagtact cgtgattgtg aaagtatctc cgactgccag gtcatttgtt tctgctagag
     5221 tagaatcaat gactacattg tttgtgcctt tgtcgctgtc atctattcct tcaccatccg
     5281 tgatttttgc tgttccatca gaaaaattac tattttgtgc agttgctgac acaccactga
     5341 tttgaaagtc tgcttgagtc atttgcggat tcccgcccat tcctccagca ggttcaccgc
     5401 ctgcatcagt agtttctgaa ctgtcttcac ttgaatcaga actagtgatg gcagttattc
     5461 catcttttgc catagctgag gcggaacttt caaaggaata actctttaca ttatctaatt
     5521 cagctatttt ttccgcgtcc gttaaggaaa ctggtgtaag agaaaaactg cctggatctg
     5581 gacgttcttc agtggtagaa ccgcttgaag aagtttgttc ctgtttttta aatgaagctt
     5641 ctcgattggc acttaaagtc actgttgctc caacttcttt ttgtgcttga ttcgctgctt
     5701 cttctgcagc gcttcgaatc gtgaggccag ctagcacaaa aataagaatg gctgtgaaca
     5761 cggcaaacaa taacaatgaa cgtccccatt ttacttttgt acttttccat gcacgtttca
     5821 taaaattcat attttgcatc cctcctagta gatgcatttg ataatagaca acctatctta
     5881 aagctagctt aaatcttatg ctgtttgcac accatcaaca tgaggttctg tatatataaa
     5941 aagtcactat agttattgca aaaaatgtgg taaaatgaaa aaagtatgtt tcaaaccgac
     6001 gaagaagaaa tgaaagagga atagcaaaag aaatcctaga tgctacagag ggatttttac
     6061 gtgcatagtt gttctaacaa ttgctgatac tagcgattta gtttatttgt tgtataataa
     6121 tggggaacag ctgaatactt atataagtat tctggctata gcgtttcgca aaagatccat
     6181 tttgttaatg aagtatatcc aaaataaaat gcccatccaa ctgtcagaac gcggatgtgc
     6241 tggtaatttt cctgctgatt ctccataagt tgagaaatag tgattttgca tatgcttcat
     6301 tggaatggct catatattta gataaagaag aaaatcgaca tctttaaact tggatgtcat
     6361 aagaagaggt gaagtttgtg agtaccatta aaattatccc attaggcggc gttcgtgaaa
     6421 atggaaaaaa catgtatgtc gcagaagtgg gcgaggatat ttttatttta gattgtgggc
     6481 taaaatatcc tgaaaatgag ttgttgggga tcgacgtcgt aatccctgat tttacgtatt
     6541 tagaagaaaa cattgatcgt gtagctggtg tctttttaac acatggacat gcagatgcga
     6601 tcggggcact tccttacttg ttgtcaaaaa tttctgtacc agtattcggg acagagttga
     6661 cgatcgagtt agcaaaattg aatgtcagtc gtaatgaagc agccaaagga tttaaggatt
     6721 tccatgtggt agatgagcat acagaaatag actttggtga aacagtagtg agctttttcc
     6781 gtaccactca tacgatacca gattcagtgg ggatcaatct gaaaacaaaa gaaggtagta
     6841 tcgtttatac gggagatttt aaatttgacc aaacggctat cccaatgtat caaacagatt
     6901 atgcacgatt ggcggaaatc ggcaaagaag gagtcttagc cttactaagt gaatcggcaa
     6961 atgcagaaaa tcctcagcct gtagcttctg aagcccaaat agcagatgaa gtatttgata
     7021 cgatcaaata ttgggaaggg cgtatcatcg tcgcttgtgt agcaagtaat ttgcaacgag
     7081 tgcaacaagt attggatgca gcagaaaaag cacaacgaaa agttgtcttg actggtcaag
     7141 atttcgaacg aattattcgc acagcgatgc gtttagaaaa acttcaatta ccaagtgaag
     7201 atctgcttgt tacgttgaaa gacatgaaaa actatgatcc agaagaactg atcattttag
     7261 aaactggccg aatgggtgaa cccatcaagt ctctgcaaaa aatggcaaac ggtacacacc
     7321 gtaatgtcaa gattgaagaa ggagatttgg tttatatcac tactacgcca agcatcgcga
     7381 tggaaaccat cgtggcgaaa acggaagata tcatttatcg agctggtgga acagtgaagt
     7441 tgatctctga gaatatgcgc gtttctggcc atgccaatcc taatgacttg caattaatga
     7501 tcaatttgat gaaaccaaca tattttgtgc cagttcaagg agagtatcgt gaattagctg
     7561 ctcatgcaga tttggctcat gcagtaggga tgccatacaa aaatatctat attactggac
     7621 gcggagacgt gctggaatat aagaacaagc gtatgagtgt tgcaggaact acaagtgcag
     7681 aaaatgtaat gattgatgga ttaggcatag gtgatatcgg aaacattgtt ttacgtgatc
     7741 gaaaaatctt gtcagaagac gggattttcg tagcggttgt cactatcagt cggcgagaaa
     7801 agaaaatcat ttcaaaacct caaattactt cacggggatt cgtttacgta aaagctagcc
     7861 gtgatctgat gaaagaaagc agcaatatga ttgaaacaat cgttgagaaa catttagaaa
     7921 gcaatgattt tgagtggagc aaattaaagc aagacatccg agatcaatta ggccgttact
     7981 tatttgaaca aacaaaacgc cgcccagtga tcttgccagt cattatggaa gctactcaac
     8041 gtcgaaataa aaaataaaca aaaagagtaa gatgaacctc tctatagagt gctcgtcttg
     8101 ctcttttttt gcgaaaaaca aataattttt gaatacagca tccctcgctt tataataaac
     8161 gatagaataa agggagtgaa tcaatgatga gtcaaacaaa agtcgaacgt tatttagaag
     8221 aacaaaagat cagctatcaa ccacttgatt ttagtcatgt ggtagaagaa tctttcacag
     8281 aagaactaca aaaaagaggg attgatccag ctttgatatg taaaactctc gtagtgaaag
     8341 gaaacaaaac tggggtgatc atcgcattga ttcctttgaa cgatcactta gattacaaaa
     8401 aaacgagaca agtgacgaaa gatcgtaaag ttggttttcc tggaatggat tttgtattaa
     8461 tgcatacagg atatccccac ggagcaaaca cccctattgg aataaaactc gcacatcctg
     8521 attattgttt tttagcagac agttctttaa aagaaaaaga agaagtaatt gtgtcaagcg
     8581 gtgaaatcgg gagaagcctt cggataaaag tagccgattt agaaaagatc gtccatccta
     8641 tttacgctga tttaattagt taagggacga gaagagaagc taagcagagc agagacctca
     8701 aacttgagat tatatctgct tggtacttgg tgcatcctgt attatctgtt acaattgctt
     8761 tgtacatact gtactatcaa tccaatgaaa atatatttag cagctatatt ttttcattga
     8821 tgtgcaagtg atgaaatcgt ttttaataaa aaagatacag agaaaacgcg aggtgtaaaa
     8881 tatgaaaatt acgtatcatg gacattcctg tttaacaatt gaaatggaaa atggccaacg
     8941 cgtgatgatt gacccgttta ttacaggaaa tccattgaca gacttaactg cagaagaagt
     9001 aaagacagat tggttgctga ttactcatgg tcatagtgac cacataggag atatgattcc
     9061 tattgcaaaa gaaaatcaag ccacaattat cagtattgtt gagattgctg attatgcgaa
     9121 cagcagagga gtagattcat tcggtatgaa tatcggtgga aaacatgcgt ttccattcgg
     9181 cgtggtaaaa ttcgtacatg cacagcatag ttcaagctat gaagtagacg gtatcgttca
     9241 gtatatgggc gagcctagcg gaattattat tcaagcagaa ggaaaaacga tttatcatgc
     9301 aggggatact gcttatttca gtgatctagg attgcttgca gaagaattcg atattgatgt
     9361 cgctttcttg ccgattggcg ataactatac gatggggcca gaagatgcag caaaggctgc
     9421 gcaaacaatt cgtgccaaga aagtagttcc aatccattac aatactttcc ctgtcattga
     9481 acaagatccc gaatcttttg tagagttact ggaagatagt gaaggaaaaa taatgagcgt
     9541 ctcggaaacg ctggaagtgt aggcagaaaa tggcgacaaa acatgatttg attttagaat
     9601 atattgaaag tttacctgtt ggcaatcgaa tttctgtaag aagcatcgca aaagaattga
     9661 atgtcagtga aggaacagcg taccgtgcaa tcaaagatgc agaaaatgtc ggactagttt
     9721 caacaattca acgagtaggg accatccgta tcgaacgcaa attaaaaaaa catattgaac
     9781 gtttgacatt tggcgaggtc gtcaggatca ttgaagggga cgttttaggc ggggcggcag
     9841 gattagacaa ggtcttgaat aaattcgtca tcggcgcgat gactaaacat gccatggaac
     9901 gctatattac gccaggatca ctaatgatcg tcggcaaccg ggcggaagtc caaaaattag
     9961 cattggaaga tggcgccgca gtattgatca caggaggatt caatacgact gctgagattg
    10021 caaagctagc tgatgatctt gaaatgccga tcttacgcac ctcttatgat acatttacag
    10081 tcgcaaccat gatcaaccga gcgttaagcg accaattgat caaaaaagat atcatgcttg
    10141 tcagtgatat ctatatgcca gctgaaaaga ctcattattt gcagcagaca gatacgattt
    10201 ctgattatca gcgtttgtca gaagaaacgc agcattcgcg ttttccagtc gtcaaccgcc
    10261 atcatcgttt gatggggatt gttacggcaa aagatgtttt agggaaatcg ccaaaccaga
    10321 tcatcgatcg ggtgatgacg aaagaaccaa tcagtgtaaa gaaaacaatg agtattgcct
    10381 ctgttagcca tcaaatgatc tgggatgggc tagaagtcat gccggtcgtt tccgatgact
    10441 tgacattgga aggcttgatt acccggcaag atgtaatgaa agcgatgcag cttgtccagc
    10501 gacaaccgca gattgcagat acgatttcgg atcagatatc tggaaatatc catacgattg
    10561 atactgaccg tgaaggaaat cgtttggaaa accctaagtt caaattcgta gtagcaccac
    10621 aaatggtcaa tggcgttgga accatttctt ttggcgtact tagcgaaatc atttctgatg
    10681 tcgcacaaaa aacaatggtg atgaaccaaa aacgaaatat cttgattgaa caagtgaacc
    10741 tacattattt gcggctgatc caattggaaa gcgaattaga cattcgtcca cgtgtattgg
    10801 aaatcggcag acgttccgct aaactggata tcgaagtgtt tattgaaaac accatcgttt
    10861 ccaaagcaat cgttgtttgt caagtaatgg aacgttctta gaaagcagga gtgaaatgac
    10921 agtacagcaa gaaatagtag atacaataaa aaaatacgat aaaatcatta tccacaggca
    10981 tttgcgccct gatcctgatg cattaggatc acaagtagga ttagccgaac tattacggga
    11041 aagtttccca gaaaaagaaa tacgtcaagt aggtgaaacg attgaagggc ttcgcttttt
    11101 agcagaaatg caagaagttg aagatgtctt ctaccaagga gcgctagtca tcgtgactga
    11161 tacagctaat tcacccagaa tcagtgatca aagatatcag ttggcagaaa aactgatcaa
    11221 aatcgaccat catcccaatg atgaccctta tggagatctt gtgtgggtaa atacaaaagc
    11281 aagtagctgt agtgagatga ttgcagaatt tgctttgatg tttcctgaat ctttaaagat
    11341 gaacgttaag gctgcacggt tactttatgc aggaatcgtc ggagatacag ggcgcttttt
    11401 gtatccctcc acgacgtccc gtacattaga gattgcttcg atattgagaa attatccatt
    11461 tgatgcaagt gctttgaaca gagagattga acaaatgccg atgaaagtcg ctaaactttc
    11521 cggttatctt tatcaaaata tccaggtgga tgaaaatggt gcaggaagaa taatccttcc
    11581 gcaagaaatt cttgataaat atggaatcga tgattcagag acagcttcga tcgtttcact
    11641 tcctggagtg attgacgaag tgctggcttg ggggattttt gttcagcagc cttcagggta
    11701 ttaccgcgta cgtttacgtt ctaaggggcc gatcatcaat gaattggcta aacgccatca
    11761 tggaggaggc catccattag caagtggtgc gaatgcaaaa gatttagcag aagtagagga
    11821 aatctataaa gagatccaag atatatgcca atcgtataag agcagaaaaa cagaatgata
    11881 cttcgttcga aaacgtgcct tttgtagtaa aaatcacaaa tgtatctttt caagatcttc
    11941 tttttccgtt acaatagtgc tcactaaaga ttttaaagga gaaagaagat gacattacca
    12001 aattgcccag aatgtggctc agagtacaca tatgaagatc gaggcttgtt gatttgccca
    12061 gaatgcggga acgaatggaa caaagaagaa caagaaactg tcatagaaga cggattagtg
    12121 gtattagatg caaatggaaa ccgtctgtta gagggcgata gcgtgacagt gattaaagac
    12181 ttgaaagtaa aaggtgcgtc aggtgccatt aaacaaggaa caaaagtcaa gaacatccgt
    12241 ttagtagaag gcgatcataa tatcgattgc aaggtagaag gatttggacc gatgaaactg
    12301 aaatcggaat ttgtgaaaaa gaattaaaga atattcaagg tcttttcaca ttcttttcct
    12361 actactaatg ataaaataaa gtaaacgaaa aggagtgaaa cgaaaatgta tggttatgga
    12421 atgtatggtt ttttagatcc gacatttctt ttggtcattc ttggattagt tatttccgga
    12481 attgcgtcag cgtacgtcaa tagtactttc cgaaaatacg atcaagtgag aagcaaaaac
    12541 aatgtgactg gtacacaagc agcacaatat atattacaaa gtcaacagat aaataatgtc
    12601 ggtgttcaac aaattgccgg agatctcaca gataactaca attcaggtaa taagatgttg
    12661 agtttgtctc aagcaactgc acagtctact tcagttgctg cgataggtgt agcagctcat
    12721 gaatgcggac atgcggtaca agatgcttcg ggttatgtcc cattgaagat tcgtgcagca
    12781 cttgttccag cagctaactt tggttcgatg atttcgttcc ccttgattat ggtaggtgta
    12841 ttgcttagct ggaactctac gttaatcaat atcgggattt tcgctttttc tttagcatta
    12901 cttttccaat tggtgacatt accagtcgaa tttaacgcgt ctcggcgagc aatccaaatt
    12961 ttgagtgaag gcggactact gacggaagaa gaagtaccga tggcaagaca cgtattattt
    13021 gcagcagcac tgacttatgt tgcagcagcg ctttcgacat tcctgcagct gttgcgcttg
    13081 atcttattgt tcggcggcaa ccgaagagat taaaataaag aagttgtgaa atcagcgctt
    13141 tgatctgagc taataccgag agttagctga ttgaatacca tttatttgga ata
//
LOCUS       DAZKLM010000076        13180 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3764
            SAMEA115706913-rid22582563.denovo.079, whole genome shotgun
            sequence.
ACCESSION   DAZKLM010000076 DAZKLM010000000
VERSION     DAZKLM010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706913
            Sequence Read Archive: ERR13196647
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13180)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13180)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287823.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287823.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,004
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,849
            CDSs (with protein)               :: 2,849
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 41 of 96
            Pseudo Genes (incomplete)         :: 52 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 15 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13180
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706913-rid22582563.denovo.079"
                     /isolate="VRE3764"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            78..686
                     /locus_tag="ACG842_002068"
                     /pseudo
     CDS             78..686
                     /locus_tag="ACG842_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333159.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            706..1734
                     /locus_tag="ACG842_002069"
     CDS             706..1734
                     /locus_tag="ACG842_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HFQ0248597.1"
                     /translation="MNRIWNKSHRNNCSLLLILLLGFILWQTPVHASEFNFAVDFELP
                     ANQIDKNKTYINVRMEPGKTQEIPYTVFNRTDKSVTVETLIHSATTNSNGVIEYGESK
                     SKFDETLSYDMKNLAKTVGEITVPPQSAVTETLSLKAPEKELAGVVAGGITFQEKKEN
                     VSNKSTVGVVNQFSQVKAILIRNSEKESSPNLKLKKTYADQSDGRNVIKANLQNPNAS
                     YMFGLTTSVEIYKKETKEPYLSIKKEKIDVAPNTNFDLLVPLEGKKLAPGHYQAVVKG
                     AWKEKRWNLHTDFEITDDAADQLNEKDIDLKNQPETFDWRWLILVGVILIGLFAGLFI
                     LKERRKKK"
     gene            1731..2489
                     /locus_tag="ACG842_002070"
     CDS             1731..2489
                     /locus_tag="ACG842_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HFQ0248598.1"
                     /translation="MKKTIALSTVLAGSLLLCTQSVFAENKSSATTEGTISVTKPDAA
                     NPEDVVPVPPVDPNDPDTPFTPNEPNQGTSGLLTIDQAPDLDFGTIKLGTAKTVYAAL
                     QNGTDSTGTATQVQNFVQVTDKSGIYAGWKLSVKRTEFIGTTSDTNKLTGSKVKFKNA
                     VIRNSATNSSGTPSTISGTGAEGIEIPADQAVDLVTAGANEGIGTWIYSLGENVEQGR
                     QSVELFVPVSNYAVDNYVSTFTWSLTDAPTEGKA"
     gene            complement(2575..3315)
                     /locus_tag="ACG842_002071"
     CDS             complement(2575..3315)
                     /locus_tag="ACG842_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytR/AlgR family response regulator
                     transcription factor"
                     /protein_id="HFQ0248599.1"
                     /translation="MKKYFIIEDNQFHSEWIIKELVNHHIENIVQLTSISQINQLIQL
                     GTIDNHDVFFIDIDLKQSLTGIDVAEQIRKKNAHSNIVFFTAYTNFAVEIINRKILPI
                     GYIRKDRNLSEQIRQVLEQINWKEQQIVNVDKIFIDQKGGSLVLYPREILYIESIKGL
                     KNQVMVKTTTEDKLVNTSLKQLKTELEKYAYFLLLKSYCINLSQLQKIDSAHYVLEFF
                     NGDSLLVGRKIFEKTKERFHDFQKTASS"
     gene            3376..4629
                     /locus_tag="ACG842_002072"
     CDS             3376..4629
                     /locus_tag="ACG842_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HFQ0248600.1"
                     /translation="MVATVTLYLTFTLILDLGIYLFFSKQLLVKNSFGFIALMVVTLL
                     FPIPKVSFPYLVGLLLMFLFYFTTKNLFISCLISNLFFAITGLSFFLVFDLMDYLGKR
                     HLGTASFFALLIAFIFYLLVKSIDKQTRFLNLLFTYPTEHYFGGICILLSWGVIYLVL
                     GSVHFRSSNYLLLSLLSIVISFFSLFFSIMLISFQVKERQHQESLQLYQINEEYYHHL
                     EEFKHDYKSLLFSLKITLQENKKETLEFLEILEDHSAAIFEKPQIQQLTNIVSPAVRG
                     VFLKFLEKAESEKIPIQVVIPVEVKTIPIDLVVFIRCLSIFISNAFDHRVADQSPITI
                     NLLEEKEGLRFSIANKANPTNLTLSEMVKRGKTSKKNGGLGLYSAKKMLDVYENAELK
                     IEFSKQNHYFFVEMYLASRKSKSAY"
     gene            4924..5178
                     /locus_tag="ACG842_002073"
                     /pseudo
     CDS             4924..5178
                     /locus_tag="ACG842_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308281.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
     gene            5300..5626
                     /locus_tag="ACG842_002074"
     CDS             5300..5626
                     /locus_tag="ACG842_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HFQ0248601.1"
                     /translation="MNSMEKVNQFRDERNWRQFHNEKDLAISISLEASELLELFQWKT
                     PEEVKETQLERIKEELADVLIYSYMMADNLQLDLDEIIEEKLIKNNLKYPVEKSAGVR
                     KKYTEF"
     gene            5694..6407
                     /locus_tag="ACG842_002075"
     CDS             5694..6407
                     /locus_tag="ACG842_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HFQ0248602.1"
                     /translation="MKKKGKAKRWLVNILLFLLLLVGLALIFNEQIKDYLVRETGDKY
                     AIANVTKEDLKKNNDKDVSFDFDAVEPMTTEGVMRSQMRGTDLPVIASIAVPSVSINL
                     PIFKGLDNTSLLYGAGTLSPDQEMGKGNYALASHRATNPELLFTPLENLEMGAKIYLT
                     DLENVYTYKTFFKEKVAPTDTQLLNEVEEKEIVTLITCGDMDAVTRLVVQGELESVTP
                     IKDATDDMRSAFNLETKTF"
     gene            6717..7569
                     /gene="licT"
                     /locus_tag="ACG842_002076"
                     /pseudo
     CDS             6717..7569
                     /gene="licT"
                     /locus_tag="ACG842_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338530.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator LicT"
     gene            7690..9555
                     /locus_tag="ACG842_002077"
     CDS             7690..9555
                     /locus_tag="ACG842_002077"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
                     /protein_id="HFQ0248603.1"
                     /translation="MDNQAVGRRVWEAVGGQKNVKSLVHCATRLRFRLKDESLADTQK
                     LKEDPDVIQVVQSGGQYQVVIGSNVADVYQSIVDEEGLAVSEDGKEESKNILNRLIDI
                     ISSIFTPFLGAMAAAGVLKGFLSLATVMGWLTADSGTYQILFAAADGVFTFLPVMLAF
                     TAAKKFKANQFLAVAIAMALVYPAITAVAGAGEAISFFGIPVILSPSGYTSSVIPIIL
                     AVWVQSKLEPFVKKVIPQFLQMILVPLVVLVVMVPLTFLALGPIGTVAGNALGGLFNS
                     IYGFSPIVAGLIMGSLWQVFVMFGMHWGFVPIMFLNIEQYGFDVLMPMLLPAILAQGG
                     AALAVALRTKDTKLRALGISSTVTSLFGITEPTVYGVTLPLKKPFIAACISGGIGGAI
                     IGFSGVKAFSSSLVSLLTIPTFINTVDGVESNVTVAVIATGIAFVLAFVGTLILGFDE
                     QTQDNQLENKHANAGEPITSARHTLKSPLTGKVLPLSEVPDQVFSSGVMGKGIAIDPE
                     VGELVAPADGEITTIFPTGHAVGITTTDGAEILIHIGMDTVELNGNGFEILVKQGDLV
                     KAGDLLIRFDIEAIRAAGYSVITPVVITNTDAFADILELDQKEIIANEDVLAIVK"
     gene            9569..11012
                     /locus_tag="ACG842_002078"
                     /pseudo
     CDS             9569..11012
                     /locus_tag="ACG842_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738852.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
     gene            11120..11821
                     /locus_tag="ACG842_002079"
     CDS             11120..11821
                     /locus_tag="ACG842_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFQ0248604.1"
                     /translation="MKRTRVLYLEVADKIKEDIFSGKYPVGSMLPTESELEELFQVSK
                     ITVRKAIELLASDEYVAKKSGRGTTVLSNRPYNRLSKAASFTQILENSRYEVRKETLD
                     LQLVHIKKEDPLFSFFGEQAVCFKRLYYLNNQPYIYFVHYLPQEMMQFSREEFGKESL
                     YRLLTKKGYTIDSFSDDFSAVFLTEEEQGILKTDAQIGIKRIRKSSTQIGEMIEYSEA
                     VYHTELHPYHIDYET"
     gene            11947..12459
                     /locus_tag="ACG842_002080"
                     /pseudo
     CDS             11947..12459
                     /locus_tag="ACG842_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014566402.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
     gene            complement(12857..13066)
                     /locus_tag="ACG842_002081"
     CDS             complement(12857..13066)
                     /locus_tag="ACG842_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanyl-tRNA synthetase subunit beta"
                     /protein_id="HFQ0248605.1"
                     /translation="MYYFKQQILVSFINEKIILFSCYVSLINDVSLNKKADELNDQNL
                     IIFVHQLLLKIFLLTINSWICKNIT"
BASE COUNT         4424 a         2207 c         2775 g         3774 t
ORIGIN      
        1 acctccagac ttcttgatag ctcaagtata tcttgaggtg catttgttag taaggtactg
       61 ttctatgggg cgcttaccca gaaaatcaac tttttgcagg agatgtatta gaaattgtat
      121 taaacgacca agcaggagaa gctgctgcta agggtgtgca gaacaagcca agtaccaacg
      181 atgaaattgg gaatcacaat ccaagtgata cgccagttgt ttatcatgat gccccgcctt
      241 ttgcacctgc tacgaagatt gtagtagaag gtggtctctc atttcttgcc ccttccaaac
      301 tcgattgtgg tcagatcaaa gcaactgggt tgacgcaaga agcatttggc caaatggata
      361 tacaggaaaa attatggatt tatgatcagc gagaaacaaa aagtccatgg atcttaacga
      421 taaaattatc tcagcctttc acaaccgatg caggattttc tatgcaggat gtcctatatt
      481 tcaagaaagc tgaggagtat caattagtga atgaagaaga actggaaatt gtcagaggac
      541 aaaattcaac gaatgtgcaa acagattatt cagaatattt aaataatggt cgggcgttta
      601 aacttgtctt aaccaaagaa aatcagatag ctggtaatta ctcagcagag atgacatgga
      661 cattagcaga tgcgccgaca gaataaagat caaggagaat gattgatgaa tcgaatatgg
      721 aataaaagtc atcgaaacaa ctgctcttta cttctgatcc ttctccttgg attcatcctt
      781 tggcagacac ctgtccatgc ctccgaattc aattttgcgg tagattttga attaccggct
      841 aatcagattg ataagaacaa aacatatata aatgtaagga tggagcctgg gaaaacacag
      901 gaaatcccct acacggtctt taaccgaaca gataaatcag taacagttga gacattgatc
      961 cactccgcta cgacgaattc aaacggtgtg atcgaatatg gagaatcaaa aagtaaattt
     1021 gatgaaacat taagttatga tatgaaaaac ttagcaaaaa cagtgggaga aataactgtc
     1081 cctcctcaaa gtgcggtcac tgagacactt tcactgaaag ctccagaaaa agagctggct
     1141 ggtgtggtcg caggaggaat cacgtttcaa gaaaaaaaag agaacgtttc taacaaaagt
     1201 acagttggtg tcgtcaatca gttttctcaa gtgaaagcaa tcttgattcg aaatagcgaa
     1261 aaagagtctt ccccaaattt gaaattaaaa aaaacttatg cggatcaaag cgatggaaga
     1321 aatgtgatta aggcgaatct tcaaaatcca aatgcttctt atatgttcgg cttgactaca
     1381 tcggttgaaa tctacaaaaa agaaacaaaa gagccttatc tttcaataaa aaaagaaaaa
     1441 atagatgtag cacccaatac aaattttgat ttacttgtcc cattagaagg aaaaaaatta
     1501 gctccgggac attatcaagc ggttgtgaag ggtgcttgga aagaaaaacg ctggaatctc
     1561 cataccgatt ttgaaataac tgatgatgca gcggaccaat tgaatgaaaa ggatattgat
     1621 ttaaaaaatc agccagaaac atttgactgg cgatggctga tacttgttgg agtcattctt
     1681 attggcttat ttgctggatt atttatttta aaagaaagaa ggaaaaagaa atgaaaaaaa
     1741 caattgcatt aagtaccgtt ctagctggaa gtctattatt atgtacacaa agtgtttttg
     1801 ctgagaataa gagctcagcc actacagaag gaacgatttc tgtaacaaaa ccagatgccg
     1861 caaatcctga agacgttgtt ccagttcctc ctgttgatcc aaatgatccc gatacgccgt
     1921 ttacacctaa cgaaccaaat cagggaacat ccggattact gacgattgac caagctccag
     1981 atcttgattt tggaacaatc aagttaggta cagcaaaaac agtatatgct gcattacaaa
     2041 acggaacgga cagtacaggg acagcaacac aagtacagaa tttcgtacaa gtaacggata
     2101 aaagcggaat ttatgccggt tggaaactat cagtcaaacg aacagaattt attggtacaa
     2161 catctgatac gaataaacta actggttcaa aggtgaaatt caaaaacgcc gtgattcgaa
     2221 atagcgcaac taatagcagt ggaacgccat ctactatttc tggtactggc gctgaaggaa
     2281 ttgagatccc agcagatcag gcagtagatc tagtaactgc aggagcaaat gaagggattg
     2341 gcacatggat ttattcatta ggagagaatg tggaacaagg gagacaatca gtggaattat
     2401 ttgttcctgt atccaattat gccgtagaca actacgtttc aacattcaca tggtcgttaa
     2461 cagatgctcc tacagaaggg aaagcgtaaa atcaaatcaa tgacttcagg gagtggagcc
     2521 gattcaaggc caactccctg aaatctgcta tgcttacgtc tcagctctta ttcatcaact
     2581 cgaagcagtt ttttggaagt catggaaacg ttcctttgtc ttttcaaaga tctttcgacc
     2641 aacgagtaag gagtcaccat tgaaaaattc taacacataa tgagcagaat caattttctg
     2701 caattgcgat aagttgatac agtaagattt taatagtaaa aagtaagcat acttttccaa
     2761 ctctgtcttc aattgtttta aagaagtatt cactaattta tcttctgtag ttgttttgac
     2821 cataacttgg tttttcaaac ctttgatact ttcaatatat aaaatttccc gaggatacag
     2881 gacgagactt ccaccttttt gatcaataaa gattttatcg acatttacga tttgctgttc
     2941 tttccaattg atctgttcta gcacttgacg aatttgttca gataaattcc tgtcttttct
     3001 gatatatcca atcggaagga ttttccgatt gatgatctct actgcaaaat tcgtataagc
     3061 ggtgaaaaaa acaatattac tatgtgcatt ttttttacgt atttgttcgg cgacatcaat
     3121 acctgtgagt gattgcttca agtcgatatc gataaagaaa acatcgtgat tatcaatagt
     3181 acccaactgg atcaactgat tgatttgaga gatagaagtg agctgcacga tattctcgat
     3241 atgatgattg accaattctt tgatgatcca ttcgctgtga aactggttat cttctatgat
     3301 aaaatacttt ttcatatgca ggctcctatt aaaaaaatat gtgtacattt tgaaacaaaa
     3361 caatggagga ataagatggt cgcaacagtg acactttatt tgacctttac tttgatttta
     3421 gatttaggta tctatttatt cttttcgaaa caactgctag taaaaaacag ctttggtttt
     3481 atagctttga tggtggtgac acttttattt cctatcccta aagtgagctt tccttaccta
     3541 gttggcttat tattgatgtt tcttttttat tttactacga aaaacctgtt tatcagctgt
     3601 ttgatcagta atcttttttt tgctatcact ggactcagct tttttctggt ttttgatctg
     3661 atggattatt taggaaagag acatcttgga acagcaagtt tttttgcact acttatcgca
     3721 tttattttct atctattagt aaaaagtata gacaaacaga cgagatttct caacttgctt
     3781 tttacttatc caactgaaca ctattttgga ggtatttgta ttttattaag ctggggcgtg
     3841 atttacttgg tattgggaag tgttcacttc cgctcatcta attatctgct tctatctctt
     3901 ttgtcgatag tcatcagttt tttttctttg tttttttcca ttatgttgat cagtttccaa
     3961 gtaaaagaaa gacaacatca ggaatcgctt caactgtatc aaataaatga agaatattac
     4021 catcatctag aggaattcaa acatgattat aaaagtctac tgttttcatt aaaaattact
     4081 cttcaggaaa ataaaaaaga aacgttggaa tttttagaaa tcttagaaga tcattctgca
     4141 gctatttttg aaaaaccgca aatacaacag ctaacaaata ttgtatcccc agctgtcagg
     4201 ggtgtctttt taaaatttct agaaaaagca gaatcggaga aaatcccgat ccaagtagtt
     4261 attcctgtcg aagtgaaaac aatcccgatt gatctggttg tttttattcg ctgtttatct
     4321 atttttattt ctaacgcatt cgatcatcgt gtagccgatc agagtccgat tacgatcaat
     4381 ctgctggaag aaaaagaggg attacgtttt agtatagcga acaaggcaaa tccgacgaat
     4441 ttgacgctat cggagatggt caaaagagga aaaacctcta aaaaaaatgg cgggttaggt
     4501 ctatacagtg cgaaaaaaat gttggatgtt tacgaaaatg cagaactaaa aatcgaattt
     4561 tcaaaacaga atcactattt ttttgtagaa atgtatctgg catctagaaa gagcaaatca
     4621 gcctactgac ttactgtcaa aggataagct tgaggaaagt ctacacggag atagagacaa
     4681 caacacagca tattcaaaag aaacagaagc gtgatgaccg ttgtcaccct tctgtttctt
     4741 ttgctatgta gttaaacaaa gtagttgaca cttccgctac ttagtattag tacgaaagta
     4801 tacttcatga tactaagtag ataagaagtg aaaagcggaa cccacgatcg tcaaaatgct
     4861 aactgctttg cttacaccag ataaagggaa gagcaatatc gcttatttta ggcgtggaga
     4921 agcaagaaaa tccctacgat cacacaaaaa atgctgaccc agcagttaag agagttagaa
     4981 caagatggca ttgtccatcg aaaagtctat aaccaagtcc cgcctaaagt agagtactcg
     5041 ctcaccgaag aaggaaaatc attaagagag attctagtag cgatgtctgt ttggggagaa
     5101 gaacgtatcg aaaaacaaaa agcctcaggg gaagaagtca cattactgca tacacatggt
     5161 ggctacctaa attactgatt tctatgacat ttattttaaa taaaagaaaa caggagtttt
     5221 tttaaaagca tcatctattt tttatgtaaa aaaatggtat gattaatcaa gtaagaaatg
     5281 attggaagaa ggcaagcaaa tgaatagcat ggaaaaagtt aaccagttta gagatgaacg
     5341 taattggcga caatttcata acgaaaaaga tttggctatt tctatttctt tggaagcatc
     5401 tgaattgtta gaactttttc aatggaaaac gccggaagaa gtaaaagaaa cacaattaga
     5461 acgaatcaaa gaagaactgg cggatgtatt gatctattca tacatgatgg cagacaacct
     5521 tcagctggat ctagatgaga tcattgaaga aaaattgatc aaaaataatt tgaaatatcc
     5581 tgtagaaaaa agcgcaggtg tacgaaaaaa atacacagag ttttagaaat gtgtcattcg
     5641 gctgttggaa agagcaaaag aaaaacagta aaatgagtct ggagggagag aaaatgaaaa
     5701 aaaaaggaaa agcaaaaaga tggctagtca atatcctctt gttcctcttg ttattagtag
     5761 gactagcttt gattttcaat gaacagatca aggattatct tgtcagagag acaggagata
     5821 aatatgctat agcgaatgta acaaaagaag atctgaaaaa gaataacgat aaagatgtca
     5881 gttttgactt tgatgcggta gaaccaatga cgacagaagg ggtcatgagg tcgcagatga
     5941 gaggaacaga tctaccagtg attgctagta tcgcagtccc ttcagtttca atcaatttac
     6001 cgattttcaa aggcttggat aatacgtcac tactttacgg agcaggaaca ttatctccag
     6061 atcaagaaat gggaaaagga aattatgcct tagcaagcca tcgtgcaacg aaccctgaat
     6121 tgttgtttac accgctggaa aatttagaga tgggtgccaa gatctatttg acagatttag
     6181 aaaatgtgta tacatacaaa acatttttta aagaaaaagt tgctcctaca gatacccaat
     6241 tactgaatga agtcgaggaa aaagaaattg tcacactaat tacttgtggc gacatggacg
     6301 ccgtcacgcg actagttgta caaggagaac tggaaagtgt tacaccgata aaagatgcga
     6361 cagatgatat gagatctgct ttcaatctag agacaaaaac attttaggat actgggacat
     6421 aagtcgttac agattaaaaa cagccaaagg atatgaatcg ataaggaaaa accaatttgc
     6481 tgaaaaaaag aggaacgcaa agaggtgcat ctctgatttg gttgttttct agatagactt
     6541 gtgttccagt ttttttattt tttatatgaa aaggcttgca taaataaaaa ttcgatggta
     6601 tgatgatttt gaataagaaa tggattgtta ctgtacggca ggcaaaacca gaattcagct
     6661 aagaaacagt aggcgaattc taggtttttt tatttttctt gagagggcga agcaaaatga
     6721 aaattgataa aattttgaat aacaatgtcg tcatttcgaa aaatggattc ggggaagaag
     6781 tcgtttgtat gggaaaagga ctagcttttc aaaaaaaaga ttggggacga gatttcgcca
     6841 gaagcggtcc aaaaagaatt tattttaaga gattcttttg ctaaaaacca attccagcag
     6901 ctgatggctg atattccggc agaggagatc gaatgggtca aacaagtagt cgaactggct
     6961 gaagataaac taaaagttga attttcgcct aatatttatc tgacattgac cgatcatctt
     7021 cattatgcga tcacacgagc aaaagaaaat atccaactgc ctaatccatt attatttgaa
     7081 acaaaaaaat tttatccgaa agaatatcaa gcagcgaaag aggctctttt gttcgtgaaa
     7141 caaaagacgg gtgtggaact ttctgaggat gaagccggtt ttattgcctt tcattttgtg
     7201 aatagccaac aaggaaacga agacatgcaa gtgacgatga ccgctaccac aatggtaaga
     7261 gatatactga gtatcatcag taaattcttc ggcatcgtgt tcgatgaaga atctttgaat
     7321 tatcagcgta tcattactca tctgcagttt tttgcccagc gttatctcaa aggggaagga
     7381 tctgacgagc aagatgaatt tctctacgca ttagttcaag gaaagtatcc aaaagcgttt
     7441 cattgtgtcg aacggatcaa tgagtatctt cgacaaacgc aaaaacagga aatggggatc
     7501 gcagagcaga tttatttaac gattcatatt caaagagtag taagtgagaa aaaaagcatc
     7561 caatcataag gattgttact gtatggcagg caaaacctgg attgaaaaaa taggttcacg
     7621 aatgtttgtg actgtttttt tcaatctggg tttttatttt ttaaataaaa aagaaagaag
     7681 gaaaaaacaa tggataatca agcagttggt cgtcgtgtat gggaagcggt aggaggacaa
     7741 aagaatgtca aaagtttagt acattgtgct acgcggttgc gattcagatt aaaagatgaa
     7801 tctttagcag atacgcaaaa gttaaaagaa gacccggacg tgatccaagt cgtacaaagt
     7861 ggcggtcagt atcaagtagt gattggcagt aatgtagcag atgtttatca atcgatcgtg
     7921 gatgaggaag gtcttgcagt ttcggaggat ggaaaagaag agtccaaaaa tattttgaat
     7981 cgtctgatcg atattatctc tagtatattt acaccttttc taggggcgat ggcagccgcc
     8041 ggggtattga aaggattctt atcattggca acggtgatgg gctggctgac agctgatagc
     8101 ggtacttatc aaatattgtt tgctgcagca gatggtgtct ttaccttctt acctgtcatg
     8161 cttgctttta cagcagctaa aaagttcaaa gccaaccagt ttttagcagt agcaattgct
     8221 atggcacttg tgtatccagc tatcacagca gttgctggtg cgggagaagc tatcagtttc
     8281 tttgggatcc cagtgattct ttcaccatct ggttatactt cttctgtcat accaatcatt
     8341 ctagccgtat gggttcaaag taaattagag ccattcgtaa aaaaagtgat tccgcaattt
     8401 ctgcaaatga ttttagttcc gcttgtcgtt ttagtagtga tggttccatt aacatttctt
     8461 gctttaggac caatcgggac ggttgccggt aatgctttag gcggtctctt caactcgatt
     8521 tatggattta gtccaatcgt agcaggtcta atcatgggaa gtttatggca agtattcgtg
     8581 atgttcggca tgcattgggg attcgtaccg atcatgtttt tgaatatcga acaatatggg
     8641 tttgatgtat tgatgccaat gcttcttcca gccatcttgg cacaaggtgg ggctgcttta
     8701 gctgtagcat tgcgaacaaa agatacgaaa cttcgagcat tagggatttc ttctacagtg
     8761 acttcgttat ttggaatcac tgaaccaact gtgtatggtg tgacattacc tttgaagaaa
     8821 ccattcattg ccgcttgtat ttctggaggt attggtggag ctattatcgg attttccgga
     8881 gtaaaagctt tctccagcag tttggtcagc ttattgacga ttccgacttt cattaatacg
     8941 gtggatggtg tagagtcaaa tgtgacagta gcagtaatag ctacaggaat tgcttttgtt
     9001 cttgcattcg taggtacatt gattttagga tttgacgaac aaacacaaga caatcaatta
     9061 gaaaacaaac atgcgaatgc aggagaaccg attacttcag cacggcatac cttaaaaagt
     9121 cctttaacgg gtaaagtact gccattgagt gaagtaccag accaagtttt ttcttctggc
     9181 gtgatgggaa aaggtatcgc tattgatcca gaagtaggag aactagttgc acctgcagac
     9241 ggagagatca cgactatttt tccaactgga catgctgtag ggatcacaac gactgacgga
     9301 gcggagattt tgattcatat cgggatggat acagtagaat taaatgggaa tggctttgag
     9361 atcttagtca aacaagggga tctcgtcaaa gcaggagatt tattgattcg ttttgacatc
     9421 gaagcaatta gagcagctgg ctatagcgta atcactccag ttgtcattac gaatactgat
     9481 gcatttgccg atatacttga attagaccaa aaagagatta ttgcaaacga agatgttctg
     9541 gcaattgtaa aataaggagg aaatcaggat gcaatcacgt ttttcagaaa aatttttatg
     9601 gggtggagca actgctgcca aatcaattag aaggggctta tgatcgagac ggaaaaggac
     9661 tatccgttgc agatgctatg cctggaggta agcagcgctt tgcgattatc ggcagtgaag
     9721 aatttgactg gacgattgat caagaaaaat atatttaccc gaatcatcga ggaatcgatc
     9781 attacgatcg tttcaaagaa gatcttgcct tgtttgcgga aatgggattc aaatgttatc
     9841 gtttttcgat tgcctggaca cgaatttttc caaatggaga cgaaggtact cctaacgaag
     9901 cgggattaga attttatgat cagctgattg atgagtgctt aaaatatgat attgaacctg
     9961 tgatcacgat ttctcattac gaaatgcctc ttcatttggc aaaagaatac ggtggctgga
    10021 aaaatcgaaa actgatcgac ttctatgaac gatttgctca gacagtatta gagagataca
    10081 gcagtaaagt caaatattgg atgactttca atgaaatcaa ttcggccttt cactttccag
    10141 ctttaagtca aggattagta aaaagcaatg gagcaggaga gtaccaaaat attttccaag
    10201 catggcataa tcaattcgtt gcaagtagca aagccgtaaa aatcggccac gaattgcgtt
    10261 cggatatcca aatcggctgc atgattatct atgcaacaac ttacagcatc gatgccaatc
    10321 cagtcaatca ggcagcaaca atgatccaaa accaagaatt caattttttc tgcacggatg
    10381 tccaagtacg tggagaatac cctgcataca cagcgcgtac ccataaaaaa tatggagtcg
    10441 atccggaaaa attagagcaa acggaaaaag acttccagtt gctaaaagaa tatccagtgg
    10501 actacatcgg atttagttat tatatgtcta cggcaatcaa tgagactgat ccacaagcag
    10561 caacttcaga agggaacttg ctgggaggag taaaaaatcc gtttcttgag gcaagtgaat
    10621 ggggctggca gatcgatcca gaagggctga gaatcgcatt gaatgaatta tacaatcgtt
    10681 accagaaacc attattcatt gttgaaaacg gtcttggagc cattgatcat atggaagagg
    10741 atggcacgat tattgatgat tatcggatcg attatttacg tcgtcatatc gaagcgatgg
    10801 ctaatgcagt agcagacggt gtagatttga tgggctatac gccttgggga tgtatcgatc
    10861 ttgtcagtgc atcaacagga gaaatgagta aacgttacgg ctttatctat gtggatctag
    10921 acgatgaagg aaatgggaca ctgaatcgat caaagaaaaa atcttttgat tggtataaag
    10981 aagtaattgc ctcaaatggc gtaaacttgt aattaaagca gctgaatgaa aaagaacaca
    11041 cttctcacat gtgatttgtg ttcttttttt attttttatg tgctattctt ttagtaatag
    11101 taaagcaaag ggtgacgtta tgaaaaggac acgtgtacta tacttggaag tcgcagataa
    11161 aattaaagaa gatattttct caggaaaata tccagtcggt tcgatgttgc cgacagaaag
    11221 tgaattagaa gaactgtttc aagtcagcaa aatcactgtc cgaaaggcaa tcgaactgct
    11281 ggcatcagat gaatatgtcg caaagaaaag tggtcgtggg acgacggttc taagcaatcg
    11341 tccttacaat cgattatcca aagcggcaag ctttacgcaa atcttggaga actcccgata
    11401 tgaagtccgg aaagaaacgc tggatttgca attggtccat ataaagaagg aagatccttt
    11461 gttttccttt ttcggtgagc aagctgtttg ttttaagcgc ttatattatt tgaacaatca
    11521 gccttatatt tattttgttc attatcttcc acaagaaatg atgcaattca gcagagaaga
    11581 atttggcaaa gaatctttgt atcgtttgct gacgaaaaaa ggatatacta ttgactcatt
    11641 ttctgatgat ttctcggcag tatttttaac agaagaagaa caaggaattc tgaaaacaga
    11701 cgcacagatc ggcatcaaac ggatacggaa atcgtctacg caaattgggg aaatgattga
    11761 atattcggag gcggtctacc atacggagct gcatccttac catattgatt atgaaacata
    11821 aataaaaaaa gaggttgtga aaaagctgcc ctgcatcaag taataagaac agccaaacaa
    11881 aaatagcttc tcatattttt cgtttggtta ggcttattac cgcagataca cggctgtttc
    11941 tggattacca tttattctgg ataagggggt gtggcacgat tttcagaagg aatctatgaa
    12001 ccaatcactg tattgatttt agcttcgatg gtaaccccat tcggcttgac gatcgcttat
    12061 ttcttaggga aaatcattcg taaaaatata ctcaatcgtc aagaaatcga tacattgaag
    12121 accgcttttc ctatggggat ctgtcagatc acagagggat gtttccctat cgtattgaac
    12181 gacttggcac gcaatgtcat agcaacggga gtcggtggga cagtaggtgg cggattaagc
    12241 atgttttggg gcgcagatag ccgtattccg gcatcgggaa tgtttgcagt cgctacgatg
    12301 accagaccat gggcattcat tggtgcattg ttagctggtt cttttgtcac aggaataacc
    12361 attttattat tgaaaaaacc agtagatccg aatgcagaaa tcatccaaga agaaaaagag
    12421 gaagaagaca tatcttggga tgatttgaca attagttagg agaggcagaa tggattacaa
    12481 gaaaaaagta caatagatgt accttgagac aggaatggta tgaaagaatc agagttagaa
    12541 agaatcgaat atacagattt tggtttagga gcgatcgaaa ctgtccagag catcggcacc
    12601 ctccttgaaa tgacgatgaa ggaaaaatga gaacctttcg ttgtcgaaag ggtctggtat
    12661 accttatgtc gaaggaaagg aaacaaaaaa actttcctgt actattccaa aaaggaaaga
    12721 aagttattat acagtaaaaa aagaaattat tcttcatgct agagatcagt acacgttcga
    12781 gcccaatatc aaacactagt tccaggcagg cgcaacaggt gcggttatca ctgaattttc
    12841 ttccagcagt gacgattcaa gtgatatttt tacaaatcca gctatttatc gttaacaaaa
    12901 agatttttag gagaagctga tgaacaaaaa tgatcaaatt ttgatcgttt agttcgtcgg
    12961 cttttttatt tagagaaaca tcatttatca gtgaaacata acacgagaac aaaatgattt
    13021 tttcgttaat aaaagaaaca agtatttgtt gtttaaaata atacacgtta ttggcatata
    13081 ggacgaaatc ccagtatctt ctataagtaa gtcatttcat atcgatgaaa acacgttatt
    13141 tatttttgtt ttatctataa aatggctgaa aaatgatttt
//
LOCUS       DAZKLM010000077        13155 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3764
            SAMEA115706913-rid22582563.denovo.080, whole genome shotgun
            sequence.
ACCESSION   DAZKLM010000077 DAZKLM010000000
VERSION     DAZKLM010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706913
            Sequence Read Archive: ERR13196647
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13155)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13155)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287823.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287823.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,004
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,849
            CDSs (with protein)               :: 2,849
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 41 of 96
            Pseudo Genes (incomplete)         :: 52 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 15 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13155
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706913-rid22582563.denovo.080"
                     /isolate="VRE3764"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..795)
                     /locus_tag="ACG842_002082"
     CDS             complement(<1..795)
                     /locus_tag="ACG842_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HFQ0248606.1"
                     /translation="MKKLKIFPKMFIQIFSVLGIIIILVHSLIFFIFPKTYLETRKEK
                     IHNMANEISSNMNGKEIKYIEQTLEFYSKSSEIKVFIKEKNNKNEIQIKDNINVNLES
                     DSNSLIIEEREIKLNDGKKTNLQFVSTADMQKDAKDLSLKFLPYSLLISILFSAIISL
                     IYAKSIKNNIQEIKIVTDKMMKLDKKMSLKVSSNDEVGELKQQINDLYSTLLRTIDDL
                     EFKNKEILKLEKLKYDFFKGASHELKTPLASLKIILENMKYNIGKYK"
     gene            complement(792..1445)
                     /locus_tag="ACG842_002083"
     CDS             complement(792..1445)
                     /locus_tag="ACG842_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFQ0248607.1"
                     /translation="MKILTVEDDNMIREGISEYLSEFGYTVIQAKDGREALSKFNSDI
                     NLVILDIQIPFINGLEVLKEIRKKSNLPILILTAFSDEEYKIDAFTNLVDGYVEKPFS
                     LPVLKARIDSLIKKNYGHLEKFEYKDLSVNFNSYTAKINGEEIDVNAKELEVLKCLLD
                     NNGQVLTRMQIIDYVWKDSEETPYDRVIDVYIKELRKKLQLDCITTIRNVGYKLERK"
     gene            1733..2253
                     /locus_tag="ACG842_002084"
                     /pseudo
     CDS             1733..2253
                     /locus_tag="ACG842_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
     gene            complement(2251..2970)
                     /locus_tag="ACG842_002085"
                     /pseudo
     CDS             complement(2251..2970)
                     /locus_tag="ACG842_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010745868.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
     gene            complement(2921..3130)
                     /locus_tag="ACG842_002086"
     CDS             complement(2921..3130)
                     /locus_tag="ACG842_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0248608.1"
                     /translation="MDVLREYSSLFTEAYLLEQFGYKIPFADYQQGVGTAAELSFYEG
                     ISQAVGRPVDAEHFEQRISSTGKSE"
     gene            complement(3112..3180)
                     /locus_tag="ACG842_002087"
                     /pseudo
     CDS             complement(3112..3180)
                     /locus_tag="ACG842_002087"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
     gene            3407..4680
                     /locus_tag="ACG842_002088"
                     /pseudo
     CDS             3407..4680
                     /locus_tag="ACG842_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250512.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(4787..5818)
                     /locus_tag="ACG842_002089"
     CDS             complement(4787..5818)
                     /locus_tag="ACG842_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0248609.1"
                     /translation="MFLEIHDLNLFFDEKQILAIDEIQIEKGELVTLLGPSGCGKSTL
                     LRCITGLEQPKSGEIVLDNENIADKPTKDRNIGFVFQQYALFPTMTVFENVAFGLKVK
                     KLSPEVIQEKVFEMLSLVDMTEQADKNVQFLSGGQRQRVALARSLVTEPKVLLLDEPL
                     SALDARIRKQLQRDLRAIQQSLGMTMIFVTHDQEEAMRISDRIFVMEAGRVAQVSTSK
                     ELYQNPQSRFVAEFIGNYNRFEWYELNQYTHEFSAKGNRCIYYLRPELIQFEPVPQGV
                     KIPVLWKESFILGNIQRYVFQTLEGKEILVDRLNDREEKISDALYIQLKDILAIPVEI
                     DTQHFAQQS"
     gene            complement(5825..6655)
                     /locus_tag="ACG842_002090"
     CDS             complement(5825..6655)
                     /locus_tag="ACG842_002090"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0248610.1"
                     /translation="MKKLIKPILFCFVLYYLLPIIGTILYASSTKWSKSLLPSDFTLQ
                     WFQQLLTDREFIAAVGRSLLLAGVVLVTILLLMIPTIIWIHLYFPRLNRWLEKLLLLP
                     YALPGVILVTALLRTYAETGIPMFVVLVGALFITALPIVYLSLNNQMRLINLKELVDA
                     AETLGAPMSTIIIQVLFPNIRIGVTLVSLMIFSSVFGEYMLTNLLIGGRFQTVRIYML
                     RRMNENGHLASAVMVLYLLFMVGIALTTFMLTYRQKQQLSLKQTREEKQSKKKPLKEL
                     "
     gene            complement(6652..7467)
                     /locus_tag="ACG842_002091"
     CDS             complement(6652..7467)
                     /locus_tag="ACG842_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0248611.1"
                     /translation="MKEMKKRLVLVPASFIFCAFLLLPLLFMVLCSLHSDLTNQWTLA
                     NYSDIFSNKYYTQAFVNSVLIAVSSSVIGLIGTFILCLCLMNLSTKLQEKITFVSNLA
                     ANFAGIPLAFSFIILLGNVGVLKMILPFLADFNLYSWWGLTITYTYFQIPLGVLFLYP
                     SMKEVKKEWLESAQLLGASSAYAWFKVALPFLRPSIASTFVILFANGMGTYETAYALT
                     SSNVNLLTIRIAALVSGDVFAKPNLGSALAVALGVLLVTAMLIIQKKGKVVQT"
     gene            complement(7464..8288)
                     /locus_tag="ACG842_002092"
     CDS             complement(7464..8288)
                     /locus_tag="ACG842_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HFQ0248612.1"
                     /translation="MQSRLDKLIVVILDGCRYDTAVAQLGFMNHLVEHQQASLLKVKS
                     ELPSNSRPLYEVLMTGVPAYENGIYSNYHAQRSKEWSLFELVKEAGGKTGAAAYHWYS
                     ELYNQAPYNRLVDRIQHQEERLIQHGIFYSEDTYPDSHLFADAHYLVNQYQPDFLVVH
                     SMNIDDVGHKFTADSHEYAAAVNQADRLLAIYLPEWLQKGYQVVVTADHGMDAHGLHG
                     GSLAAHREVPFYLISNKRQKLKEQTVPQLLAAPLFCWLLGIAPSEKMQDIQELMRG"
     gene            complement(8278..9378)
                     /locus_tag="ACG842_002093"
     CDS             complement(8278..9378)
                     /locus_tag="ACG842_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFQ0248613.1"
                     /translation="MKMNEKIRNMTIGLFCAATVSILLTGCGSGEATKQSAAADDSLD
                     TIIEKAKEEGEIASLGMPDTWANWVGTWNDLEKEYGLSHVDTDMSSAEELAKFESEGK
                     NGTADIGDVGISFGPLAESKELTLPYKTNYWEEIPDWAKDDDGDWLLSYTGTISFITD
                     KENVKQAPTSWKELQEGDYQVSVGDVTIANQAQFAVLAAAIANGGDEKDIQPGLNYYK
                     KMAEKGRLSTVDASVANLEKGEIDVAVVWDFNGLNYRDQIDSERFEVTVPTDGSIISG
                     YTTLINKNAPHPNAAKLAREYILSDEGQINLAKGYARPIRKVELPKEVAEKLLPEDAY
                     EAAVPVEDNEAWNKTSVELPELWQAEVLTYAK"
     gene            9556..10341
                     /locus_tag="ACG842_002094"
     CDS             9556..10341
                     /locus_tag="ACG842_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HFQ0248614.1"
                     /translation="MQYYDQHLHTYFSFDSEEKFENYLAYQPEFFVSTDHFDLNNPST
                     GKDDIPDYPAYINKLDSLKETYSTKFLRGIEIGVVPGQEERIVDYLAQHPYDLKLVSI
                     HQNGRFDYMDDVVLTKDPIQTTKEYFDQMLQVLTDFPEGDILTHFDYGLRRFTFTAEE
                     FAEHFEAQLLTIFKKVVERELAMELNAKSFLKYGNEDLYRYAVPLYQSVGGKLFTLGS
                     DAHVAEDYQLGFKKMSQLLADCQVEKLLVIQGKERRLTPLPIL"
     gene            10374..10757
                     /locus_tag="ACG842_002095"
     CDS             10374..10757
                     /locus_tag="ACG842_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HFQ0248615.1"
                     /translation="MSNLWDYNQEAPIHYLIARHWDALKIEAVCRSLLAAVPKQQLEN
                     FLVADSLQREKVQAYFAAFKDQPLEYLHAQFHLFYQVAAPDDYNDLRGQLQLTFQADE
                     TAYTVLLGMARLGDQAKVEWRIFDI"
     gene            10833..10964
                     /locus_tag="ACG842_002096"
     CDS             10833..10964
                     /locus_tag="ACG842_002096"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFQ0248616.1"
                     /translation="MLVTDAGEIRLRLFPDQAPLAVANWRSLAEQGVYDNTPFGSCH"
     gene            10933..11169
                     /locus_tag="ACG842_002097"
     CDS             10933..11169
                     /locus_tag="ACG842_002097"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFQ0248617.1"
                     /translation="MTTHLSARVIKEFVIQGGALDGSGDEAVSSYEGFFADEVHRGLY
                     HFNGALALGDHGPHTNGNQFFIVQNTKAQADLLM"
     gene            11181..11267
                     /locus_tag="ACG842_002098"
                     /pseudo
     CDS             11181..11267
                     /locus_tag="ACG842_002098"
                     /inference="COORDINATES: protein motif:HMM:NF012387.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
     gene            complement(11247..12218)
                     /locus_tag="ACG842_002099"
     CDS             complement(11247..12218)
                     /locus_tag="ACG842_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HFQ0248618.1"
                     /translation="MEGTVLRIEQGSLHDGAGLRTVVYLKGCPLRCAWCSIPESQSKQ
                     IEKGFGQTMTAEEVMDEIEKDAVFYFHSDGGVTISGGEALVQADFAKEILQKSKYIGI
                     NTVLETSFCGAYNEIQKVAPYVDTLFVDVKMFTSKLHKQWTGLDNQQILKNIRRFLIE
                     YPNCEVRIRVPVVPGINMNLTELLTIACFVADLDRFVPLELLPYHCYGMHGYQALGLE
                     YPLADTPAPSPKEMFALADQLARTVPHLLVTALTKTFIYLLNPSFILWINERHSLAAA
                     SSRIELLLKSVSVFLRCFFEVNLSNEHWLNRYIFTVCRLSCDRCYDF"
     gene            12870..>13155
                     /locus_tag="ACG842_002100"
     CDS             12870..>13155
                     /locus_tag="ACG842_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008177227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFQ0248619.1"
                     /translation="MMSKNNTNGRNQFAMLTIDDLVPQDHLVRKIDAALDFEFIYPIV
                     EATYSDLGRPSIDPVILIKLVFIQYLFGIRSMRQTIKEVDTNVAYRWFLGY"
BASE COUNT         3959 a         2900 c         2348 g         3948 t
ORIGIN      
        1 tttatatttt ccaatattgt atttcatgtt ttccagtatt attttaagac tagcaagagg
       61 ggttttaagt tcatgggatg cacctttaaa aaagtcatat tttaactttt ctaattttaa
      121 aatttcttta tttttaaatt ccaaatcgtc aattgttctt aataaagtag aatacaaatc
      181 attaatttgt tgttttaatt ctccaacttc atcattagag ctaactttta aactcatttt
      241 tttatcaagt ttcatcattt tatcagtaac aatttttatt tcttgtatat tattttttat
      301 tgattttgca taaattaaag aaataatagc agaaaataaa attgatatta ataaagaata
      361 tggtaaaaat ttaaggctta aatctttcgc atctttttgc atatcagctg tagaaacaaa
      421 ttgtagattt gtttttttac catcattaag ctttatttct ctttcttcaa ttatcaaaga
      481 attactatca ctttctaaat taacatttat attatctttg atttgtattt cattcttatt
      541 atttttttct tttatgaata cttttatttc actactcttg gaataaaact ccaaagtttg
      601 ttctatatat tttatttctt ttccattcat attactagaa atttcatttg ccatattatg
      661 aatcttttct tttctcgtct ccaaatatgt tttaggaaag ataaaaaaaa ttaatgaatg
      721 aactaatata attattattc caagaacaga aaatatttgt atgaacattt ttggaaatat
      781 ctttaatttt ttcattttct ctccaattta tatcctacat ttcttatagt agttatacag
      841 tctaattgta atttttttct aagttctttt atatatacat ctattactcg atcataagga
      901 gtttcttcgc tatctttcca tacataatca ataatttgca ttcttgttaa cacttgtcca
      961 ttattatcca ataaacattt taatacttct agttcttttg catttacatc tatttcttca
     1021 ccatttattt tagctgtata gctattaaaa ttaaccgata gatccttata ttcaaacttc
     1081 tctaaatgtc cataattctt cttaattaaa gaatctattc tagcttttaa gaccggcaat
     1141 gaaaatggct tttctacata tccgtctact aaattagtaa atgcatcaat tttatattct
     1201 tcatcactaa atgcagtcaa gattagaatt ggtaaattgc ttttctttct aatttctttc
     1261 aacacttcta aaccatttat aaaaggtatc tgaatgtcta agataaccaa atttatatcg
     1321 ctattaaatt ttgacaaggc ttctcttcca tctttagctt gaataacagt atatccaaat
     1381 tctgaaagat attcacttat tccctctctt atcatattat catcttcaac agttaatatt
     1441 ttcatatata cctccactta agcctataca ctagatatta tacagcatat tatggctcta
     1501 taatatctag tgttggtgac gccttctgtt gccaatactt tttctttttt ttaataaatg
     1561 caaacagaca ccttgaaatc ctttagaaaa aaggcttcta taatattttg tgttgttgga
     1621 aaatcgaagg cgattttcta ctaacacttt ttcttcgttt caactcaaaa agagactcac
     1681 ttttggtaaa atttagtcga ccaaaacatc accctaccaa aggagtcgct ctatggataa
     1741 tcatactaga aaattactcg gattaacaga taaacattta ttttttgatg aagaatggct
     1801 gattgaaaaa gaatataaag gcgttcaagc gcagtttatt aaagggagat tggatgactc
     1861 accttcccat tgtgaacact gtggatcgat tcgtattatc cgcaacggtt cctatacgac
     1921 acgtactcag atgttgaaag tcaaagaaaa gttgactatt ttagaattaa aacgtacccg
     1981 ttttctatgc tatgattgtg ggcagacctt ttctgcgaaa acggacttag ttgatgaaca
     2041 tcatcaacta acaaaggaac tgaagcaggc gattctcata gaactgtacg aaaatcaatc
     2101 acgtaatctg aaaatcagaa gaagtaccgt cggctcaagc gatattggaa actcttactc
     2161 aaagattcca cgacattgga gccgctcaaa cggcattatc atcgactgtt taaacgacca
     2221 attagtcaaa cagaaatcgt cgatgaaccg ttgtgcacaa tggtgtcctg aacgtacagc
     2281 gacaccttcc atatccagag cagtcgccac atcacgggga tggatgccat ccaaattaaa
     2341 agcaaccacg ccagtatgat gttcaggatc ttgcggtccg taaatggtga taccgccgat
     2401 cgcttgtaat ttaggtaaaa cgtaggcaac cagttcttgc tcataacgat gaatattctc
     2461 cataccaatc tcattcaagt aatcgattgc tcctgcaatg ttcggagtac cagcttcaaa
     2521 tttccatggc agctctttcc atgaactatc gtaaagattg acaaaatcta tcatttcacc
     2581 gccgaattcg actggctcca tttgttctaa caattgacgt ttgccatata atacaccgat
     2641 tcctgtcggc ccgcacattt tatgcccgct aaaggcgaaa aaatctgcat ccaacgcttg
     2701 tacatcaact gtcatatgcg gtgctgcttg ggcaccgact ggactatcct caactacaac
     2761 agcctgttca accctcagcc gtttcaagca caacaaataa ccttctggat ctggtttgcc
     2821 gacctgaaga tcatcgatcg tcacaaagga atcaattttt tccttaatca tcggatgtgc
     2881 ttcaaaaaaa tagtctagct cccgcagttc agaattgaga ctactccgat tttccagtgc
     2941 tgctgatccg ctgctcaaaa tgttcagcat ccactggtct cccaaccgct tgacttatcc
     3001 cttcataaaa ggacagttct gctgccgtcc caacaccttg ttgatagtct gcaaaaggaa
     3061 ttttatagcc gaactgctcc agtaaatagg cttcagtaaa tagtgatgaa tactctcgta
     3121 aaacatccat tcactgtcaa tcatcgtccc atcaaaatca aaaataagtg ctttggtcat
     3181 cttctttccc ccttacccaa aacaagcaaa agctgtgtcc tgctctctct acagtatcag
     3241 cataaaaact gccgtctgaa aagtcaaagg tgaaagaaat gtggcgctat aatttctagg
     3301 actgatagta aatcattcta tcagtccttt tgagcataaa aaaacaaaac atacagaagc
     3361 tccgttagaa ttgaatcacc acaacacaat caaaggagcg ttctgtatgc ctgatcttca
     3421 tcttatcaaa ctattactag gaatcaaaga tggaaacatt caaatcactc atgtcgagaa
     3481 gaaaaaaata cattcaatca acagtatcgt tgtttctgcc acgctcatta aaacgattca
     3541 gccctgtcct cagtgtaaac aggtgaatca acatgggctg atcgttaaga acggaaagaa
     3601 aaaatcgctg gtccttctca ataagtgtgc gaatcaacgg acttatcttg ctttggcaaa
     3661 gcaacggtac ctctgtcgtg gctgtcacac ctattttacg gcgaatacct acattgttga
     3721 aaaaaactgt tttatcgcca aacaagtaca ttataaaata ttggaggaat tgactgaaaa
     3781 acaagcaatg acgacgattt ctaaacattg cggcgtttct tggtcaaccg tttcgcgaac
     3841 actcgcttct ctgctcccca tgacaaacgt gaaaataaat tggttgcctc gctgttcatt
     3901 ggtagatgaa tttcgttcat tgaaaaatca agtcggtccc tattcttttt cttgtatgga
     3961 tggcgatact gggaaattac tggatattct tccttcacgt aagaagaaag atctcatttc
     4021 ttattttatg caatttgaac gacgtgcacg tttaaacgta aaaatactcg taacagatat
     4081 gaacgcctct tatgctttgc tgataaaaga gtgctttcct catgccaaac tcgttgtgga
     4141 tcgctttcac atcgtcaagc acctcattcg cagttttaag gatatttgac tatgagtcat
     4201 gaaaagcttc gaccgaaacg atccaataca agccaaacac tatcgacaag tgaaggcttt
     4261 atctcgactt ctgatcaaac gacaggacac gcttgtttat gataaatgga caaaatggcg
     4321 taattttggt tgggcctatt taaccgaaag tgaagtcgta gaacgtctac tttctacttc
     4381 tgatgaacta cgaatagctt atgcttacta tcaagaaatt cttcaagctt tttatgacaa
     4441 agaagcggac atcttctttc agcttgtaaa aacaatgccc aagtcggttc caagagaact
     4501 gcatcatata aaaaaagcgt ttataaacta tgaatccggc attcgtttag ccttagagtt
     4561 gccttactca aatggcaaaa tagaaaatct gcatacccac atcaaagcat tgaaacgaat
     4621 agcttacggg ttaggatgag aaccagaatt ttcttaatca atcatctgat cacttattaa
     4681 acataaaaaa acatctaaag cagaaaatca attctacctt agatgttctt atatcactct
     4741 atcagtccta tttgacaaag agccagaaat gtttgccgac tatacactag ctttgttgtg
     4801 caaaatgctg agtatcaatt tccacaggga ttgctagaat atctttcagc tgaatgtata
     4861 aggcatcgct aattttctct tcccgatcgt tcaaccgatc cactaaaatt tctttgccct
     4921 ccagcgtttg aaaaacatac cgttgaatgt tgcctaaaat aaatgattcc ttccatagaa
     4981 ccggaatctt aaccccctga ggaaccggtt caaattgaat cagctccggt cttaaataat
     5041 aaatgcagcg attgccctta gcggaaaact cgtgggtata ctgattcagc tcataccatt
     5101 cgaaacgatt atagttgccg ataaattccg caacaaatcg gctctgagga ttctgataca
     5161 attccttcga ggtggaaacc tgagccaccc gcccagcttc catcacaaaa atccgatcac
     5221 tgattcgcat cgcctcttct tggtcatgag tcacaaaaat catcgtcatc cctagcgatt
     5281 gctggatagc gcgcaaatcc cgctgcagct gttttctaat tcgggcatcc aaagcactta
     5341 atggctcatc cagcagcagg actttcggtt cagtgaccaa tgagcgagcc agtgccaccc
     5401 gttgtcgttg accgccagaa agaaactgga catttttatc tgcctgctcc gtcatatcca
     5461 ccaatgatag catctcaaaa accttttcct gaatcacctc aggactcagc ttctttacct
     5521 tcaaaccaaa ggctacattt tcaaaaacag tcatagttgg aaacaatgca tactgttgaa
     5581 aaacaaagcc gatattgcga tccttcgtag gcttatcggc aatattctca ttgtccagca
     5641 caatctcacc gctctttggt tgttccaatc ccgtgataca gcgcagcaac gtagactttc
     5701 cgcacccgct gggacctagt aaagtaacca gctcaccctt ttcaatctga atttcatcaa
     5761 tagctaaaat ctgtttttca tcaaaaaaca gattgagatc atgtatttct aaaaacattt
     5821 tccgtcacaa ctcctttaag ggcttctttt tcgactgctt ctcttccctc gtctgcttaa
     5881 gggacagctg ttgcttttgt cgataagtca gcataaaggt agttaaagca attcccacca
     5941 taaacagcaa atacaaaacc atcacagcgc tagccaaatg gccgttttcg ttcatccgcc
     6001 gcagcatata gatgcgtacc gtctgaaatc gaccgccaat caacaggttc gtcagcatgt
     6061 actccccaaa aaccgaagaa aaaatcatca gagaaactag ggtcacaccg atacggatgt
     6121 ttggaaacag cacctggata ataatcgtag acatcggggc gcccaaggtt tctgctgcat
     6181 ccactaattc cttcagatta attaagcgca tctggttgtt aagactaaga tagacaatcg
     6241 gcaacgccgt aataaacaat gcacctacca acactacaaa cattgggata ccagtttcag
     6301 catacgttcg aagcaaagcc gttaccaaaa tcactcctgg taaagcatac ggcagcagga
     6361 gcagcttttc cagccaacga ttcaaacgag ggaaatacaa atgaatccaa ataatggtgg
     6421 gaatcatcaa caacagaatc gtcaccaaga ccacacctgc taataaaagt gagcgaccca
     6481 ccgcagcaat aaattccctg tccgtcagca gctgttggaa ccattgcaag gtaaaatcac
     6541 taggtaaaag cgattttgac catttagtgg agctggcata caaaatcgtt ccaataatcg
     6601 gcagcaagta atacaggaca aagcagaaca aaatcggttt tattaacttt ttcacgtctg
     6661 caccaccttg cctttctttt gaataatcaa cattgccgtt accagcagga cacctaaagc
     6721 gactgccaac gcactgccta aattaggctt ggcgaacaca tctcccgaca ctaaagccgc
     6781 aattcgaatc gttaaaagat ttacattact gctggtcagg gcatacgctg tttcataggt
     6841 ccccatgcca ttagcaaata agatgacaaa ggtgctggca atcgatggtc gcaaaaaagg
     6901 caaagccacc ttgaaccaag catacgctga tgaagcgccc agcaattgtg cggactccag
     6961 ccattccttt ttcacttcct tcatgctggg atacaaaaac aagaccccta acggaatttg
     7021 gaaataggta tacgtgatgg tcaatcccca ccaagaatac aaattgaagt ccgctagaaa
     7081 tggcaaaatc attttcagca cacctacatt ccccaacaaa ataataaaag agaatgccag
     7141 cggaatccca gcaaaattag ctgccaaatt ggaaacaaag gtgattttct cctgcaattt
     7201 agtcgataga ttcattaagc ataaacataa aataaacgtc ccgattaacc caatcacact
     7261 ggagctgaca gcaatcagta cgctgttaac aaaggcctgt gtgtaatatt tgttgctgaa
     7321 aatatcactg taattggcca atgtccactg gttagttaaa tcactgtgca agctgcacag
     7381 aaccataaaa agcagcggca gcagtaaaaa tgcacagaag ataaaggagg caggaaccag
     7441 aaccaagcgt tttttcatct ctttcatccc ctcatcaatt cttgaatgtc ctgcattttc
     7501 tctgacggtg caattcctaa taaccagcaa aacaatggag ccgccagcag ctgtggtaca
     7561 gtttgttcct tcagcttttg gcgtttattt gaaatcaaat aaaaaggaac ttcacgatgt
     7621 gcagccaagc tgccgccatg cagtccgtgc gcatccatcc cgtgatccgc agtcaccacg
     7681 acttgataac ctttttgcag ccattcagga aggtaaatag ccaataatct gtccgcctga
     7741 ttaaccgctg ctgcatattc atgagaatcg gcggtaaatt tgtgaccgac atcatcaata
     7801 ttcatcgaat gtacgaccaa aaagtctggc tgatactgat tcactaaata atgggcatcc
     7861 gcaaataaat gcgaatcagg gtaagtgtcc tcactataaa agatgccatg ctgaatcagt
     7921 cgctcctcct gatgctggat gcgatcaacc aaacgattat agggggcttg attgtacaac
     7981 tcgctgtacc agtggtaagc ggcggctcct gtttttccgc cagcttcctt aactaattca
     8041 aacagcgacc actctttgga tcgctgagca tggtaattac tatagatgcc attttcatag
     8101 gctggaacgc cggtcatcag tacttcgtat aacggacgtg aattgctggg cagctcactt
     8161 tttaccttca ataagcttgc ttgctggtgc tcaactaaat gattcatgaa cccaagctgg
     8221 gcgacggctg tgtcgtagcg acagccgtct agtataacta caatcagttt gtcaagtcta
     8281 ctttgcatag gtcagtacct ccgcctgcca aagctctgga agctctacag aggtcttgtt
     8341 ccaagcttca ttgtcttcaa ctggcaccgc cgcttcataa gcatcctccg gcagtaattt
     8401 ttcagctact tctttaggca gctctacctt gcgaataggt cgcgcatagc cctttgccaa
     8461 attgatttgc ccttcatcag ataaaatata ttcccgtgct agctttgccg cattgggatg
     8521 aggggcattt ttattgatta aggttgtata cccgctaatg atggagccgt ctgttggcac
     8581 agttacctca aaacgctcgg agtcaatttg gtcacgataa tttagtccat tgaagtccca
     8641 aacaactgcc acatcgattt cacctttttc aagatttgct acgctggcat caacggtgga
     8701 caaacggcct ttttctgcca tttttttgta gtaattcaga cctggctgaa tatccttttc
     8761 gtcacctccg ttggcaatcg ccgcagccaa tacagcaaat tgtgcttggt tggcaatcgt
     8821 cacatccccc acgctaactt gatagtcacc ctcttgcagc tccttccagc tagtcggtgc
     8881 ttgcttcaca ttctctttat cggtgataaa ggaaatggtt cccgtatagc ttaacaacca
     8941 atccccatca tcatcctttg cccaatccgg aatttcctcc caatagttgg tcttataagg
     9001 taaggttaac tctttgcttt cagccaacgg accgaagctg ataccgacat caccaatatc
     9061 ggcggttcca ttttttcctt ctgattcaaa cttagccaat tcttctgcac tggacatatc
     9121 cgtatccacg tgacttaagc cgtattcttt ttcaaggtcg ttccaagtgc ctacccaatt
     9181 tgcccaagta tccggcattc ccaagctggc aatctctcct tcttccttgg ctttttctat
     9241 aatcgtatct aaagagtcgt ctgccgcagc cgattgcttc gttgcttcac ctgagccgca
     9301 gcctgtcagc aggatactta ctgttgctgc acaaaataaa ccgatagtca tgttacgaat
     9361 tttttcattc attttcattc attcttctcc cctttcgata attccattct aacaaagctt
     9421 tgtaatgaga gaatcagttt cttgaaaagg tttcatgaac aatgtaaagg ttgtatatgg
     9481 tttgtaaatt tcgacgaatt cccgttaacc ttctaaagct tttgcggtat gatagtagaa
     9541 aaaaggaggc tccctatgca gtattatgat cagcatctcc acacgtattt ttcctttgat
     9601 tcagaggaaa aatttgaaaa ctatttagcc tatcagccgg agtttttcgt ttctacggat
     9661 cactttgatt taaacaatcc atctaccgga aaagatgaca ttccggatta tccagcctat
     9721 ataaacaaac tggactcttt aaaagaaacc tattccacca aatttctgcg aggtatcgag
     9781 attggcgttg ttccaggaca agaagagcgg atcgtggatt acctcgctca gcatccttat
     9841 gatttaaagc tggtaagtat tcatcaaaat ggccgctttg attatatgga cgatgtggtt
     9901 ttaaccaaag atcccattca aactaccaag gaatattttg atcagatgtt gcaggtcttg
     9961 acggattttc cagaaggaga tattttgact cactttgatt atggattacg ccgcttcacc
    10021 ttcacagcgg aagaattcgc ggagcatttt gaggctcagc tgctaacgat tttcaaaaag
    10081 gttgttgaac gagaattggc gatggagtta aatgccaaga gcttcttgaa atacggcaac
    10141 gaagaccttt accgttatgc cgttcctttg tatcaaagtg tcggcggaaa gctctttacc
    10201 ttaggctcgg atgctcatgt tgccgaagat tatcagctgg gctttaaaaa aatgagtcag
    10261 ctgttggcag actgtcaggt agaaaagttg ctagtcattc aaggaaaaga acgccgcctg
    10321 acgccgctgc cgattttgtg attcctatat aatagaagaa agtggtgaaa aaaatgagca
    10381 acctgtggga ctacaatcaa gaggcaccca tccattattt aattgcgcga cattgggatg
    10441 cgttgaaaat tgaggcggtt tgccgatcct tgttagcagc cgttccaaaa caacaactgg
    10501 aaaatttttt ggtggctgac agcttgcagc gggaaaaagt tcaggcttat tttgcagcct
    10561 ttaaagatca gcctttggaa tatttgcatg cgcagtttca tcttttttat caagtagccg
    10621 ccccagatga ctacaacgat ttacgaggac agctgcaatt gacctttcaa gcggatgaaa
    10681 ccgcctatac cgttttattg ggaatggctc gccttggtga tcaggcgaag gtcgagtggc
    10741 ggattttcga tatttgatgg caggatcaag gcgtttcagt tgcaaacctt cctctctttc
    10801 aattagaaaa accgcagcag gaagaagttt gtgtgctggt caccgatgct ggtgaaattc
    10861 gtctccgctt atttcctgac caagctcccc tggctgtagc caactggcgc tccctagctg
    10921 agcaaggcgt ttatgacaac acacctttcg gctcgtgtca ttaaggaatt tgttattcaa
    10981 ggtggcgctt tggatggttc tggtgatgaa gctgtttcca gctatgaggg ctttttcgct
    11041 gatgaagtgc atcgcggact ttatcatttt aatggcgcct tggccttagg ggaccacggc
    11101 ccccacacca acggcaacca gttttttatt gtgcaaaaca caaaggcgca ggctgacctg
    11161 ctaatgtaaa gcaagcatac ttggagcttg gaggactacc ggagctggat ggtcgttata
    11221 ctgtttttgg acaggtatac caagggttag aaatcgtagc agcgatcgca gctcagccga
    11281 caaacagtga agatgtaccg attgagccag tgttcattcg acaaattaac ttcgaaaaaa
    11341 catcgtaaaa agacactcac tgacttcagc aaaagctcta ttcttgagct ggctgcagct
    11401 agtgagtgtc tttcatttat ccataagata aagcttggat tcaataaata aataaatgtt
    11461 ttggttaaag ctgtgaccag caaatgagga accgtgcgag ccagttgatc tgccaatgca
    11521 aacatttctt taggtgaagg cgctggcgtg tctgccaacg gatactccag tcccaatgct
    11581 tggtaaccgt gcataccata gcagtggtac ggtagtaatt ctaacggtac gaaccgatca
    11641 agatctgcca caaaacaagc aatcgtcagc agctcggtca ggttcatatt gatacctgga
    11701 accacaggaa ctcgaattcg cacttcgcag ttaggatact caattaaaaa gcgacgaata
    11761 ttcttcaaaa tttgttggtt atctaagccg gtccattgtt tgtggagctt gctggtaaac
    11821 atttttacat ccacaaacaa tgtatccaca tagggagcta ccttttggat ttcattataa
    11881 gcaccacaaa agctggtttc cagtacggta ttgattccaa tgtatttcga tttttgcaaa
    11941 atttctttag caaaatccgc ctgaaccagt gcctcgcctc cgctaatggt cactccgcca
    12001 tccgagtgaa agtaaaaaac agcatccttc tcaatctcat ccattacctc ttcagcggtc
    12061 atggtctggc caaatccttt ttcaatctgc ttgctttgtg attcgggaat cgaacaccag
    12121 gcacagcgta atgggcagcc ctttaaataa acaacggtcc gtaaaccagc accatcatgt
    12181 agagaaccct gttctattct taatactgtt ccttccatat agatttttga attacacact
    12241 agtttaaact aaaacacaca aggaaaaact taccatttaa tcataaaatt agctcatata
    12301 aatatactag gatgtaaaat agactggtca aaatctggtc aaaaaagagg actaatacag
    12361 gtagtcctct tttttgttat tttaatattc taaatcattt tttattggtt tattccctta
    12421 ttcgatgtag actcaaatca ctatatcact agatatcaaa cggcgtcata cggaatcaaa
    12481 aacatcaaat ttgacctgtt aaatggtcat tgaataacat tcaaaatcaa taaaacggag
    12541 tcagacacgg agtcaaaaac gctgagagta aaaaatatat acagccttgt aggtaaagta
    12601 ccacagggct gtatattttt tatttcagat taccttaaat gattccattt actttttatc
    12661 tcattttttc ctactagtta gtatcaaata ctctcctatt ttctcaataa actatttttg
    12721 gatacatggt atctgaaaca gctaaaacct tgtcattaca gcaaaaaatc acgttatcca
    12781 gactgtagac aaagtccatt ttatggatga tgtctacagt cttttctttt ataatagatt
    12841 tatgaaaacg aatgcaagga gtgatagcta tgatgtctaa aaacaatacg aatggtagaa
    12901 atcaatttgc gatgcttacg attgatgatc tagttcctca agatcatctc gttcgaaaga
    12961 ttgatgccgc gcttgatttt gaatttattt atccaatcgt cgaagccact tactccgatt
    13021 taggtcgccc aagtatcgat ccagtgattc tgatcaaatt ggtgtttatt cagtatttat
    13081 tcggtatccg ttccatgcga caaaccatta aggaagtcga taccaatgtc gcttaccgct
    13141 ggtttttagg ctact
//
LOCUS       DAZKLM010000078        12925 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3764
            SAMEA115706913-rid22582563.denovo.081, whole genome shotgun
            sequence.
ACCESSION   DAZKLM010000078 DAZKLM010000000
VERSION     DAZKLM010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706913
            Sequence Read Archive: ERR13196647
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12925)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12925)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287823.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287823.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,004
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,849
            CDSs (with protein)               :: 2,849
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 41 of 96
            Pseudo Genes (incomplete)         :: 52 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 15 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12925
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706913-rid22582563.denovo.081"
                     /isolate="VRE3764"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..375
                     /locus_tag="ACG842_002101"
     CDS             <1..375
                     /locus_tag="ACG842_002101"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0248620.1"
                     /translation="VKDNRNSIRKEVLHLIKFVKKTVAIPSEKKINESTRKLYRRANK
                     KALKLSAFLSSLSLKLSLILFISEHVSITDFLNLAKNPYLKGVRSILWQGLQSLACLE
                     VRTWFSYSSCRKIFLMMILSIN"
     gene            complement(324..893)
                     /locus_tag="ACG842_002102"
     CDS             complement(324..893)
                     /locus_tag="ACG842_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HFQ0248621.1"
                     /translation="MELKKLEVPTSSITEVKRSPMDVFAQARDAGTGVYIFNREKVAG
                     VMLTQEQYETLLQELDVLRQTVKVERKEPKEINEEPINKTASVVSNAEGMEELAQTLQ
                     HSLITGVTITAKNLDERMVALGFITKKTGFGGVVDMLSELMETGKINYQLRRKPQNRT
                     IIAEIIGEQNSQSQLMDKIIIKKIFLHEL"
     gene            complement(967..2256)
                     /locus_tag="ACG842_002103"
     CDS             complement(967..2256)
                     /locus_tag="ACG842_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0248622.1"
                     /translation="MRWQKLLSKNAQNLFSLINLMKDLIWRKADLAEELKIDVKTVDR
                     HLKRLAESDCPIQLILHKKTVQLVYDQGYSEPYLLFVFLIQSPPFCLLKNLLLNEPYE
                     QKYDRSTQERLRKWFGEYHLSFSYKKAQIFGSERKVRYLLFCFLKDFPHFFADDGEQS
                     FSEMFIQLLKQRKDPAEIEILGDSRTLFFELFPELLFREEQILPIKEKNYLAILRLSC
                     NQITEKRWKRIEETQDYRKLTIVTEKLDSLFWLNSEFESENKSKLTKVLYQEWLRYVI
                     GIHDRLIEEVRYYQETLEKFPNSKDKLYQFRLAIQQAFAHPAVEWGMLLLKTLQERGY
                     LDVHPPEVKIVFFFRYEHQEARRLAGVLNKTLRHRKRISIHVCTRNKPPRYADLVITD
                     HFFPLDHNEDQKTYLYHPSFGIERLLTDINYWLGKNR"
     gene            2514..3458
                     /locus_tag="ACG842_002104"
     CDS             2514..3458
                     /locus_tag="ACG842_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFQ0248623.1"
                     /translation="MEEKIEESKQKIDQLIQELEKAVIGKRSTIQLMIVALLAGGHVL
                     FEDIPGVGKTLLVKALAKAVQGTFSRVQCTPDLLPSDILGFSVYNSQSKEFEFRPGPI
                     FTTILLADEINRTTPRTQSALLEAMAENHATIDNNTYPLDNHFFVLATQNPIEYEGTY
                     PLPEAQLDRFLFRLQIGYPSFDDELLLLLDKFEKVLDNLNEVLNSYEIEEIKTNVANV
                     FVSPEVASYALQLVSATRSHAAIQLGISPRGSLSFIQAAKAYALIHGRNYVTPKDLQD
                     LVPYVFSHRLIFYDRSLKEDEKKQIIHTLVAQIPVPVR"
     gene            3472..4545
                     /locus_tag="ACG842_002105"
     CDS             3472..4545
                     /locus_tag="ACG842_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HFQ0248624.1"
                     /translation="MEKIKDYFINFVLFLVYFIIVLFALVFNNSLGWFLLAFFTLYVS
                     WSLISLSSFLKKLSVTCTLQPLVYRKKSMQVIFSIAKKRTFSLPLPRLQIGFPAIFAE
                     KKQEILILTNKPQEVQLLWQPKERGCHETLTVIFTAYDSLGLFRKSAKREISFPVTVL
                     PAFCKIQAEKLQRVLEKKQQLNAYQRGLDFREHRAYRPGDSFNRIDWKLSAHTQEWLY
                     REYDYQEEECSPLFCFWGSHSPKFEPLLDLYFSLWHLRKEKQPELLILGNRAFHGKDP
                     GHTYFASLEAGDEKAFFAHLKKYAKKQIVLFVPENSPEVSEAIETLSKDRTVYLVYFV
                     EKQLMVQEKDKICSLPGGEWIDD"
     gene            4535..6706
                     /locus_tag="ACG842_002106"
     CDS             4535..6706
                     /locus_tag="ACG842_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HFQ0248625.1"
                     /translation="MTIKWEKWLCGVYSFVILSVGAPIFVVANQLQNSYLLILTIGVL
                     CLIVCCIPKKWVRLLLSLPVMIGCLFLYFPSKTFSLLWILQFFSSVTEEFFHLSQGKL
                     YYLPEKTAFFLIMLLIYLFITLTVDYDYLYAPFLSLMAYFMMLTVFREKDLLYEMIAV
                     VTVLFVYLAARQFFSMRLLSGNTRFQSLTTVLLICFLTISAILPLYLPKVHQSLEETS
                     EPIREYLNDEGLYDTLHEYQLTGSARTGFSENDEQLGGPLYDNGEVVFTANQKGNGYW
                     KIENKNHYTGSGWIEDQDAPIQPIQEFPVDITSYQTVMEDKENITLTLKKQAFLPYPY
                     GNTTLTDFSLASGQTLYYLPESQRFIPVPNESTRSATLQTERLVFTREELKSSQLKAD
                     DLENQQTNLQLPKSLPKRIEDLAVELTKNEATLIRKVEAVEQYLKTSGNFRYSKTDTG
                     HTPKEHDYVDYFLFDSKVGYCDNFSTSMVVLLRTLGIPARWTKGFTNGLRTGTDNQGN
                     EEYSILNSHAHSWPEVYFPGFGWLPFEPTPSFANPMGSQPVAETTETVQPAIEQPNES
                     ENMAETISSERSEVIPSEETTLTPAYKIVLFSLGVCLYVLLFFLLLFRKSLRWTSYLI
                     YLHFFASTNTIAYQKLLNHLETLVPRKPAESLQEYSLRIEQEFKLDLRFIRLTTQYEA
                     EIYGRQADPVNFDKKTYLEITHIIRKTVYKKVLTKGSRFKK"
     gene            complement(6818..8989)
                     /gene="copB"
                     /locus_tag="ACG842_002107"
     CDS             complement(6818..8989)
                     /gene="copB"
                     /locus_tag="ACG842_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper/silver-translocating P-type ATPase CopB"
                     /protein_id="HFQ0248626.1"
                     /translation="MNGNDMEKKHEHKKEVVNKESTESHEKQMEMKHDHSQMDHSMHM
                     GHNHGDIANSKQMDHSMHMDHDHGGMDHSMHMGNFKQKFWLSLILAIPIILFSPMMGM
                     EFPFQVTFPGSDWLVLILATILFIYGGQPFLSGAKMELKQKSPAMMTLIAMGITVAYI
                     YSVYSFIANLLNPHTHVMDFFWELATLIVIMLLGHWIEMNAVSNASNALQKLAELLPE
                     SVKRLTKDGKEETVSLKEVNEGDRLIVRSGDKMPTDGVILKGETIVDESAVTGESRGI
                     KKQANDKVIGGSINGDGTIEIEVTGTGENGYLAKVMEMVRKAQGEKSKLESLSDKVAK
                     WLFYVALIVGILAFIAWLFLTDLPNALERMVTVFIIACPHALGLAIPLVVARSTSIAA
                     KNGLLLKNRNALEQANDLDVIMLDKTGTLTEGKFTVTGVEVLDDAFNKNEILQYLGAL
                     EANANHPLAVGIMNYLKEHEIKPYQAENLKNLSGVGLEATVKNQQVKIVNEKEVERLG
                     LNVEQALLKPYQEQGNTISFLILENHLAAIVALGDVVKTEAKEFIRTLKERKITPVML
                     TGDNKNAAQAVADYLGIEEYYGGLLPDDKEAVVQKYLDQGKKVIMVGDGINDAPSLAR
                     ASIGMAIGAGTDIAIDSADVVLTNSDPKDILHFLDLAKQTRKKMIQNLWWGAGYNILA
                     IPLAAGILAPIGIILSPAVGAVLMSLSTVVVALNALTLKIK"
     gene            complement(9009..11195)
                     /locus_tag="ACG842_002108"
     CDS             complement(9009..11195)
                     /locus_tag="ACG842_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFQ0248627.1"
                     /translation="MATAETKMDTFVITGMTCANCSARIEKELNGQPGVVNATVNLAT
                     EKASVKYEGTTTEKLIQSVENIGYGAILYDEAHKQKIAEEKQAYLKKMLFDLILSTVL
                     TLPLMLSMIAMMLGSHAAIVHFFHFPIVQLVLSAPVQFYVGARFYKGAYHAIKSKAPN
                     MDVLVAIGTSAAFALSIYNGFFRGHPQDLYFESSSMIITLILLGKYLEHTAKTKTGNA
                     IKQLMSLQTKTAQVIRNGKEETLAIEEVVVGDQLVIRPGEQIPADGRIISGSSAIDES
                     MLTGENLPVEKNPDDTLFGGTINTNGLLHMEVTQVGKQTVLAQIIQMVEDAQGSKAPI
                     QKIADRISGIFVPIVLVIAFITLIATGLITGDWQLALIHSVSVLVIACPCALGLATPT
                     AIMVGTGVGARNGILIKGGEALEAAAHLDSIVLDKTGTITEGKPKVTDLVGSKEVLSI
                     FYTLEQASEHPLGKAIVEYGKLQEAATYDMIDFTAHPGAGISGTINGVRYFAGTRKRL
                     IELNLSFDEYQEHALELEQQGKTVMFLADEKQVIGLIAVADQIKLEAKQAIKQLQNKG
                     LDVFMLTGDNKLAAETIGKQVGIDPKHIFAEVLPEDKAAYVEKLQKDGKKVGMAGDGI
                     NDAPALALADVGMAMGSGTDIAMETADVTLMSSSLASIAQTIELSRVTLRKIKQNLFW
                     AFVYNTIGIPFAALGFLNPIIAGGAMAFSSVSVLLNSLSLNRHMKK"
     gene            complement(11195..11404)
                     /gene="copZ"
                     /locus_tag="ACG842_002109"
     CDS             complement(11195..11404)
                     /gene="copZ"
                     /locus_tag="ACG842_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone CopZ"
                     /protein_id="HFQ0248628.1"
                     /translation="MKQQFSIEGMSCNHCAARVEETVSALDGVQKVKVNLKKANGTVK
                     FDETKVQSEKICQAINGLGYKAEVI"
     gene            complement(11417..11857)
                     /locus_tag="ACG842_002110"
     CDS             complement(11417..11857)
                     /locus_tag="ACG842_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006825 - copper ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopY/TcrY family copper transport repressor"
                     /protein_id="HFQ0248629.1"
                     /translation="MEKVKTPVKISDSEWEIMRVIWTLGQTTAQEITQILADSMDWKP
                     ATIKTLLGRLVKKEVIWTEQEGKKFIYHPAVSEMETVRSATENLFSHICAKRIGETIA
                     DLVEEATLTQEDINEILKQLEQKQPVDTIECNCIPGQCECKQHQ"
     gene            complement(11931..12429)
                     /locus_tag="ACG842_002111"
                     /pseudo
     CDS             complement(11931..12429)
                     /locus_tag="ACG842_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853035.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="topology modulation family protein"
     gene            12631..>12925
                     /locus_tag="ACG842_002112"
     CDS             12631..>12925
                     /locus_tag="ACG842_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HFQ0248630.1"
                     /translation="MNDSIKKMLRLIEKDLMITEVSYETFQKKKTLIVDAVFSPAPHT
                     CRNCGSTVVDGNGKVIVVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCRTH"
BASE COUNT         3668 a         2703 c         2303 g         4251 t
ORIGIN      
        1 gtcaaggaca atcggaatag catacggaaa gaagttctgc accttataaa atttgtcaaa
       61 aaaactgttg ccattccaag tgaaaaaaag atcaatgaat caactagaaa actatataga
      121 agagcaaata aaaaagcact caaattgagt gcttttttaa gctcactatc actaaaacta
      181 tcactaattt tatttataag cgaacatgtt tctatcactg attttttaaa tctagcgaaa
      241 aacccttatc ttaaaggcgt tcgttcaatt ctttggcaag gtcttcaaag cctggcttgc
      301 ctagaagtgc gaacatggtt ttcttatagc tcatgtagaa aaattttctt gatgatgatt
      361 ttatccatca attgagattg actgttttgc tctcctatga tttcagctat gattgtccga
      421 ttttgcggtt ttcttcttaa ctggtagttg attttcccag tttccatcaa ctcgctaagc
      481 atatccacga ctccgccaaa tccggtcttt ttcgtaataa aacctagtgc taccattcgt
      541 tcatctagat tttttgctgt aatcgtcaca ccagtaatga gcgagtgctg taatgtttgg
      601 gccagctctt ccatcccttc agcattggat acgacggaag ctgttttgtt aataggttct
      661 tcgtttattt cttttggttc tttcctctcc actttcactg tttgacgaag gacatctagt
      721 tcttgcaaga gcgtttcgta ttgttcttgg gtcagcatca cgccagctac tttttcacga
      781 ttaaatatat atacgccagt tcccgcgtct cgcgcttgag caaatacatc catcggcgat
      841 cgtttcactt ctgtgatcga agatgtggga acttctaatt tctttaattc caatggattt
      901 cctcctttac taatcgcttt ttattatagc acagactgac ttgttttgat cttttttcca
      961 aaggttttat cggtttttgc ctaaccagta attgatatcg gtaagaagtc tttcgatacc
     1021 aaatgaagga tggtacagat acgttttttg atcttcgtta tggtctagtg gaaaaaaatg
     1081 atcggtgatc actagatctg cataccgagg cggcttattt cgagtacata catgtataga
     1141 gatccgtttt cgatgtctta gcgttttgtt cagtacccca gcaagtcttc ttgcttcttg
     1201 gtgttcatag cgaaaaaaaa agacgatttt cacttcagga gggtggacat ctaaataccc
     1261 tcgttcctgg agtgttttca gtaaaagcat cccccactct actgcggggt gtgcaaatgc
     1321 ttgttggatc gctagccgaa attgatatag tttatctttg gaattgggaa atttttctaa
     1381 tgtttcctga taataacgaa cttcttctat cagccggtca tggataccaa ttacgtaacg
     1441 aagccattct tggtaaagta cttttgtcag ctttgatttg ttttcgctct caaattcgct
     1501 attcagccaa aacagcgaat caagcttttc agtcacaatc gtcaatttcc gatagtcttg
     1561 tgtctcttct atccgtttcc accgcttttc agtaatctgg ttacagctca agcgcaaaat
     1621 tgctagataa tttttttctt ttattggcaa aatctgctct tctctaaata gcaattcagg
     1681 aaacaattca aagaatagtg ttcgactgtc tcctaaaatc tcgatttctg ccggatcctt
     1741 tctttgcttc aataattgga tgaacatctc agaaaaggac tgctctccat catctgcaaa
     1801 aaaatgcgga aaatctttca aaaagcaaaa cagcagatat cttacttttc tttctgaacc
     1861 aaagatctgt gcttttttat aagaaaaaga aaggtggtac tcgccaaacc actttctcaa
     1921 tcgctcttgc gtagaacgat cgtacttctg ctcgtaaggt tcattcaata gcagattttt
     1981 cagtaaacaa aacgggggtg attggatcag aaaaacaaac aagagataag gttcgctata
     2041 tccttgatca taaactaact gaacggtttt cttatgaaga atcagctgga tgggacagtc
     2101 gctttccgcc aaacgtttca gatgccgatc cactgtttta acatcgattt tcagttcttc
     2161 agctaaatca gcttttcgcc agatcaaatc tttcattaga ttgattagtg aaaataaatt
     2221 ctgtgcattt tttgataata gtttttgcca tctcatcgat catttctcac ctgatttcta
     2281 aacatattgt ttgatcaaaa tgggttcact cctaaaaaga taaaaaactc cctttctatt
     2341 tatttaaata cgcaaatttt cctcattatt tttaatttta ctcaatttaa tcgtattctc
     2401 atttcaaaac gaatgtactt taaaaaaaca taataaaatc actttgcttg taggatgtat
     2461 tcccttccat tacaatgaaa taaatagcaa ttttatgaga aggtgatgtg aaaatggaag
     2521 aaaaaatcga agaatccaaa caaaaaatcg atcagttgat acaagaatta gaaaaagcgg
     2581 tcatcggcaa acgttcaact atccaattga tgatcgtggc cctattagca ggtggtcatg
     2641 tcttattcga agatattccc ggtgtaggta aaacgttact ggtcaaagca ctagcaaaag
     2701 ctgtacaggg aaccttcagc agagtccaat gtactcctga tctgctgcct agtgatattc
     2761 ttggtttttc cgtatacaat tcacaatcaa aagagtttga atttcgtccg ggaccgatct
     2821 ttaccacgat cttgttggca gatgagatca atcgcacgac tcctcgtact cagtcagcac
     2881 ttttagaagc aatggccgag aatcatgcaa cgatcgacaa caatacatac cctctagaca
     2941 atcatttttt tgtcttagct acgcaaaatc caatcgagta tgaaggaaca tatcctttgc
     3001 cggaagcaca gctcgatcgt tttttattcc gactacaaat cggttatcct tcttttgatg
     3061 acgaactttt gctactttta gataaattcg aaaaagtgtt ggataactta aatgaagtat
     3121 tgaacagtta tgaaatagaa gaaataaaaa caaatgtggc gaatgtcttt gtcagtcccg
     3181 aagtggcatc ttatgccttg cagttagtgt cagcaacaag aagccatgct gccatccagc
     3241 ttggcatcag tccacgagga agcctttcct ttattcaggc agcaaaagct tatgctttaa
     3301 tccatgggag aaattacgtc acgcccaaag atttgcagga tttagttcct tacgtgtttt
     3361 cccatcgcct gattttttac gatcgttcat tgaaggaaga cgaaaaaaaa caaatcattc
     3421 atactcttgt tgcacaaatt cctgttcctg tgaggtagat tgaggtagat aatggaaaaa
     3481 atcaaagatt atttcattaa ttttgtgtta tttctggtat atttcatcat tgtgcttttt
     3541 gctcttgtat tcaacaattc tttaggctgg ttcttgttgg ccttttttac tctttatgta
     3601 tcatggagct tgatttcatt aagcagcttt ctaaagaaac tttcggttac ttgtacattg
     3661 cagccactcg tttatcgcaa aaaaagtatg caggttattt tttccatagc aaaaaagaga
     3721 actttttctt tacctctccc tcgtttacaa atcggcttcc cagcaatctt tgcagaaaaa
     3781 aaacaagaga ttttgattct tacaaataaa ccgcaagaag tccaattgct ttggcagcca
     3841 aaagaaagag gatgccatga aacattaact gtcattttta cagcctatga tagtctagga
     3901 cttttcagaa aaagtgcaaa aagagagatc tcttttcctg tgactgttct gcctgctttt
     3961 tgtaagattc aagcagaaaa attacagaga gttctggaaa aaaaacagca gctgaacgcg
     4021 taccaaagag gtcttgattt tcgggaacat cgcgcttatc gtccaggtga ttctttcaac
     4081 cgaatcgatt ggaaattgtc tgctcatact caagagtggt tataccgtga atacgattat
     4141 caagaagaag aatgttctcc tcttttttgc ttttggggtt cgcacagtcc aaaatttgaa
     4201 cctctgctag acttgtattt ttctttatgg catttgagaa aagaaaagca gccggaactt
     4261 ctgatattag gcaaccgcgc ctttcatggc aaagaccccg gtcataccta ttttgcttct
     4321 ttggaagctg gcgatgagaa agcttttttc gcacacctaa aaaaatatgc aaaaaaacaa
     4381 atcgtcctct ttgttcctga gaattctcct gaagtctcag aagctattga aacattgtca
     4441 aaagatcgaa cggtttatct tgtttatttc gtagaaaaac aactcatggt ccaagaaaaa
     4501 gacaagatat gttcattgcc aggaggtgaa tggattgacg attaaatggg aaaaatggct
     4561 gtgtggcgtt tactcctttg tgatactaag tgtaggtgct ccgatatttg tagtagctaa
     4621 tcaattgcaa aacagctacc ttcttatcct aactattggt gtcctatgcc ttattgtctg
     4681 ctgtattcct aaaaaatggg tacgtttgct gctttctttg ccagtaatga taggttgttt
     4741 gtttttgtat tttccttcga agacattttc cttattatgg atcttacagt ttttttcgtc
     4801 agtcacagag gaattcttcc atctatccca aggtaaactt tattatttgc cagaaaagac
     4861 agcttttttt ctcataatgt tgctgatcta tttatttatt acattgaccg tggattacga
     4921 ctatttgtac gcaccctttc tttctttgat ggcttacttc atgatgctta ccgtttttcg
     4981 ggaaaaagat ttactgtacg aaatgattgc ggtagtaact gttttgttcg tgtatctggc
     5041 agcaaggcaa tttttctcca tgcggttgtt gtcgggaaac actcgattcc agtcattgac
     5101 tacagttctg cttatttgtt tcttaactat tagcgctatc ttgcctttat atcttccgaa
     5161 agtacaccaa tctctcgagg aaacgagtga accgatccga gaatatctta atgatgaagg
     5221 gctttatgat acactccatg agtatcaatt aaccggaagt gcaagaacag gcttcagtga
     5281 gaatgatgaa cagcttggtg gacctttata tgataatggc gaagttgttt ttacagcgaa
     5341 ccaaaaagga aatggctatt ggaagatcga aaataaaaat cattacaccg gttctggatg
     5401 gatagaagat caggacgcac ccatccaacc aatccaagaa ttccctgttg acatcacgag
     5461 ctaccaaacc gttatggaag acaaagaaaa cattacattg accctaaaaa aacaagcttt
     5521 cttaccttat ccttatggaa atactacctt gactgatttt tcattagcta gtggacaaac
     5581 gctttactat ttgcctgaaa gccagcgatt cattcctgtc ccgaatgaat cgacacgttc
     5641 tgcaacttta caaacagaaa ggttagtttt tacgagagaa gaattaaaaa gcagtcagtt
     5701 aaaagctgat gatttagaaa atcagcagac aaatcttcaa ttgccaaaaa gtttgcctaa
     5761 acgtattgag gacttggcgg tcgaattgac caaaaatgaa gcaactttaa ttcgtaaagt
     5821 cgaagctgtc gaacagtatt taaaaacaag cggaaatttc cgctattcca aaacggatac
     5881 cggacatact ccgaaagaac acgattatgt ggattatttc ctctttgatt caaaagtcgg
     5941 ctattgtgac aacttttcaa cttctatggt cgtccttctt cgaacacttg gtatcccagc
     6001 tcgctggaca aaaggtttta caaatgggct cagaacaggc acagataatc aagggaatga
     6061 ggaatacagc attctcaata gtcatgctca ttcttggcca gaggtatact ttcctggatt
     6121 tggctggctt ccatttgaac caaccccttc atttgccaat ccaatgggtt cacaaccagt
     6181 tgctgaaaca accgaaaccg tccagcctgc tatcgaacaa ccaaatgaaa gcgaaaatat
     6241 ggcagaaact atttccagtg agagatctga ggtcattcca tcagaagaaa caacactcac
     6301 accagcttat aaaatcgtct tattcagcct tggggtgtgt ttatacgtct tgttgttctt
     6361 tctattgtta ttcagaaaat ctctgcgctg gacaagttat cttatctatc ttcatttttt
     6421 tgcttctact aatacgattg catatcaaaa acttcttaac catttggaaa cattggtacc
     6481 gcgcaaaccc gcagaatctt tgcaggaata tagcctgcga atagaacaag aattcaagct
     6541 agatctgcgt ttcattcgat taactactca atatgaagca gaaatatatg gacgacaagc
     6601 agatccggta aatttcgata aaaaaaccta tttagaaatt acgcatataa taagaaaaac
     6661 ggtatacaaa aaagttctga caaaagggag ccgcttcaaa aaataagata aaatttctgg
     6721 agaatctact tctggaatgc cgagaaccag ctgcttgagt atcgattatt cgaaaaaaag
     6781 gttgtgacac gttttttgtc acaacctttt ttcattatta tttgattttc aatgtcaaag
     6841 catttagagc tacaactacc gtacttaacg acatcaagac agcaccaact gctggactta
     6901 atatgatacc aattggcgca agaattccgg ctgctagcgg aattgcaaga atattgtaac
     6961 ctgcccccca ccagaggttc tggatcattt tctttcgcgt ttgcttggct agatcaagaa
     7021 aatgcaaaat atcttttgga tcgctgtttg tcaatacgac atccgcagaa tcgattgcga
     7081 tatctgtccc tgcaccgatt gccatcccga ttgaagcacg ggctaaactt ggcgcatcat
     7141 tgatcccatc acccaccatg attacttttt tcccttggtc aaggtatttc tggacaacag
     7201 cttctttatc atctggtaat aaaccgccat aatattcttc gatccctaaa taatctgcta
     7261 ctgcttgggc agcgttttta ttgtcaccag taagcatgac aggagtgatt tttctttctt
     7321 tcaatgttcg gataaattct tttgcttctg tttttactac gtcacctaaa gcaacgattg
     7381 cagctagatg attttctaaa atcaggaagc tgatggtatt tccttgttct tggtaaggtt
     7441 tcaataatgc ttgttctacg tttaatccga gacgctcaac ttctttttca ttaactattt
     7501 tcacttgttg gtttttgact gttgcctcta gcccaactcc tgaaagattt tttagatttt
     7561 cggcttgata aggcttgatt tcatgttctt tcaaataatt cataatacca acagccaatg
     7621 gatgattggc attcgcttcc aatgctccta aatattgaag gatttcattt ttattgaacg
     7681 catcatcaag tacttcgaca cccgttacag taaattttcc ttctgttagt gttcctgttt
     7741 tgtccagcat gatcacatct agatcattgg cttgctccag tgcattgcgg tttttcaaaa
     7801 gtaatccgtt tttagctgct attgatgtag agcgagcaac aactaatggg atagctaaac
     7861 ctagagcatg aggacaggcg atgataaaga ctgttaccat acgttctaat gcattaggaa
     7921 gatctgtcaa gaacaaccag gcgatgaatg ccaggatacc cacaattaat gcgacataaa
     7981 acagccattt tgccactttg tcagataatg actctagctt ggatttttct ccttgtgctt
     8041 ttcgcaccat ttccataacc ttcgctagat aaccattttc accagttcca gttacttcga
     8101 tttcgatcgt tccatcccca ttgatagaac cgccaatcac tttgtcattg gcttgttttt
     8161 tgattcctct tgattcgcca gtcacagctg attcgtcaac aattgtttca cctttcaaga
     8221 ttacaccatc tgttggcatt ttatcaccag accggacgat caaacgatca ccttcgttta
     8281 cttctttcaa tgaaactgtc tcttcttttc catctttcgt caagcgtttt actgattctg
     8341 gcagtaattc tgctagtttt tgtaaagcat tgctggcatt cgacacagca ttcatttcaa
     8401 tccagtgacc tagtagcata ataacgatca aagtagccaa ttcccagaag aaatccatga
     8461 cgtgtgtatg tggattgagc agatttgcga taaacgaata gacactataa atatatgcaa
     8521 ctgtaatccc catcgcgatc agtgtcatca tagcaggact tttttgtttc aattccatct
     8581 tagctccact taaaaatggc tgaccgccat agataaatag tatagttgct aagatcaata
     8641 cgagccaatc agaaccagga aacgttactt gaaatggaaa ttccattccc atcattggtg
     8701 aaaaaagaat gattgggata gctaggataa gcgatagcca aaatttttgt ttgaaatttc
     8761 ccatgtgcat ggaatgatcc atgccgccat gatcgtgatc catgtgcatc gaatggtcca
     8821 tttgtttgct gtttgctata tctccatggt tatgtcccat gtgcatggaa tggtccatct
     8881 ggctatggtc atgtttcatc tccatctgtt tttcatgaga ttctgtactt tctttgttta
     8941 ctacttcttt tttatgttca tgttttttct ccatatcgtt cccattcatt tttactccat
     9001 cccttctctt actttttcat gtgccgattt aagctcaggg agttcaacaa tacactgacg
     9061 gaactaaacg ccatcgctcc tcctgcaatg attggattca aaaagcctag tgcggcaaaa
     9121 ggaataccaa tcgtattata gacaaaagcc cagaaaagat tttgtttgat ttttctcaat
     9181 gtcaccctag acaactcgat tgtttgtgca atggatgcca aactgctact catcagcgtc
     9241 acatcggctg tttccatcgc gatatccgtt ccacttccca ttgccatacc aacatcagct
     9301 aatgctaaag ccggtgcatc attgattcca tcgcctgcca taccgacttt tttgccatct
     9361 ttctgaagtt tttctacgta ggctgcttta tcctctggaa gtacttcagc aaagatatgc
     9421 tttggatcaa taccaacttg tttaccaatc gtttcggctg ccagtttgtt gtctcctgtc
     9481 agcataaaaa catctaatcc tttattctgg agctgcttga ttgcttgttt ggcttctagt
     9541 ttgatttgat cagccacggc aatcaacccg atcacttgct tttcatcagc taaaaacatg
     9601 actgtttttc cctgctgttc taattctaac gcgtgttctt ggtattcatc aaatgatagg
     9661 ttcaattcaa ttaatcgttt acgtgtaccc gcaaaatagc gtactccgtt aatcgtaccg
     9721 ctgattccag cacctgggtg agctgtaaaa tcaatcatat cgtatgttgc tgcttcttgc
     9781 agttttccgt attccacgat tgctttacct agtggatgtt cagaagcttg ttctaaagta
     9841 taaaaaatag ataggacttc tttagaaccg actagatctg taactttggg ctttccttct
     9901 gtaatcgtcc cagttttatc caaaacaatg ctgtctaagt gagcagctgc ttctaatgct
     9961 tctccgcctt taattaaaat tccatttcgt gcaccaacac ccgttcctac cataatagct
    10021 gttggagtcg ctaaacctaa tgcacatgga cacgcaatga ctaatacaga gacactatgg
    10081 atcaatgcga gttgccaatc accagtgatc aaacctgtag ctatgagcgt gataaacgcg
    10141 atgactaata cgatcggaac aaaaatccct gagatccgat ctgcgatttt ttggattggg
    10201 gctttgctcc cttgagcatc ttccaccatt tgaatgattt gcgctaatac tgtctgcttg
    10261 ccgacttgtg tgacttccat gtgtagcaga ccattcgtat tgattgtccc gccaaaaagc
    10321 gtgtcatccg gattcttttc tacaggaaga ttttcaccag taagcatact ttcatctatc
    10381 gcactgcttc ctgaaataat tcgaccatct gctggaatct gctcaccagg acggatgact
    10441 agttgatcac caacgactac ttcttcaatc gctaacgttt cttctttccc gtttcttatc
    10501 acttgggcgg tttttgtttg aagagacatc aattgtttga ttgcattacc tgttttcgtt
    10561 tttgctgtat gttccaaata tttgccgagc aaaatcagcg tgatgatcat actgctgctt
    10621 tcaaaataaa gatcttgggg gtgtccacgg aaaaatccgt tgtaaatgct caatgcaaag
    10681 gcagccgatg ttccaattgc taccaataca tccatatttg gtgctttcga cttgattgca
    10741 tggtacgcac ctttataaaa acgagcaccg acataaaatt ggacaggagc agacagcact
    10801 aactggacga ttggaaaatg aaaaaagtga acaatcgccg catggcttcc taacatcata
    10861 gcaatcattg acaacatcaa cggcaaagtc aaaaccgtac tcaatataag atcgaatagc
    10921 atttttttca aatacgcttg cttttcttct gcaatttttt gtttgtgggc ttcatcgtac
    10981 aaaattgcac catagccgat gttttctacg ctttggatta atttttcagt ggtcgtccct
    11041 tcatatttga cacttgcttt ttcagtggcc aagttgactg tggcattgac cacaccaggt
    11101 tgtccgttca gctctttttc gatcctagca gaacaatttg cacaagtcat acctgtaatg
    11161 acgaacgtat ccattttggt ttctgctgtt gccatcaaat cacctctgct ttataaccca
    11221 atccattgat tgcttgacaa atcttttcgg attgaacttt tgtttcatca aacttgaccg
    11281 taccgtttgc ttttttcaaa tttactttca ctttttgcac gccgtcaagt gcgcttactg
    11341 tttcttctac tcttgctgca caatgattgc agctcatacc ttcgattgaa aattgttgtt
    11401 tcattttttc ttcctcctat tgatgttgtt tacattcaca ttgacctggg atacagttac
    11461 attcgatcgt atctactggt tgcttttgtt ccagttgttt caaaatctca ttgatatctt
    11521 cttgagtcaa agtggcttct tctactagat ccgcaatcgt ctctccaatc ctttttgcac
    11581 agatatggga aaaaagattt tccgtcgcac tgcgaactgt ttccatctcc gagacagctg
    11641 gatgataaat gaattttttt ccttcttgtt ctgtccagat cacttctttt tttaccaaac
    11701 gacccagcaa agttttgatc gttgctggtt tccaatccat cgaatcagct aagatctgcg
    11761 tgatttcttg ggctgtcgtc tgtccaagtg tccaaataac acgcatgatc tcccactctg
    11821 aatcactaat ctttactggc gttttgactt tttccattta tttcacacct ttcgtttaca
    11881 aatgtaatca ttaacttatg aatacagatt acacttgtaa acaaaaactg tcaattattt
    11941 ttgctctcta ttttcaattt agttaaaaat tcttgtttct ctcgtcttgt tttaaggatg
    12001 atcagtttat ttcttgcatt agctacttgg atcagctgct gtatttttgg ttcattgttt
    12061 ccttcaaaat gccagataaa agataaaaat tctaagtatt tcctgtttag tttctccgaa
    12121 aatgattcag gcatatctgg ccgagatttt ttgtttatct ttctttttag agaacgccaa
    12181 actactcggt agatggcgag ataacgcggc actttaagcc aaatgatctt atctgcttct
    12241 ttaatccttt cctctaacgt agaagtataa tttccttcaa cgatccatga cggattagat
    12301 accataaacg attgctgttc ttgcaaaaac cacttttttg cctgaatgcg gaaagccggt
    12361 gatttctttg atcttccttg cgagggacga ttttcccgac ccactttgtc caatcacgat
    12421 gatcttcatt cttctccctc acttctcttt ggaatttggt tcgatcatac cacttataac
    12481 gccgcaaaaa aacaggctct ataatttatg ggactgatga atcagaattg attcttcagc
    12541 cccattttta ttttaggtat taaaaaacat atcgttctct agtatgatta aatcacgaca
    12601 aaataaacaa ctgagaggac gatatgctat atgaatgatt ctatcaaaaa aatgctgcga
    12661 ttaatagaga aagatttgat gattacagag gtctcttacg agacctttca gaagaaaaag
    12721 acgttgatcg tcgatgctgt tttctcacct gctccccata cctgtagaaa ttgtggttct
    12781 actgtggtag atggaaacgg gaaagtaatt gtagtgaaaa atgggaagaa ggaaacgatt
    12841 gtccgctttg aacaatacaa tcacatgcct ttggttatgc gcttaaaaaa gcagcgctat
    12901 acctgtaaaa actgtcgaac ccatt
//
LOCUS       DAZKLM010000079        12910 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3764
            SAMEA115706913-rid22582563.denovo.082, whole genome shotgun
            sequence.
ACCESSION   DAZKLM010000079 DAZKLM010000000
VERSION     DAZKLM010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706913
            Sequence Read Archive: ERR13196647
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12910)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12910)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287823.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287823.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,004
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,849
            CDSs (with protein)               :: 2,849
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 41 of 96
            Pseudo Genes (incomplete)         :: 52 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 15 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12910
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706913-rid22582563.denovo.082"
                     /isolate="VRE3764"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            127..3654
                     /locus_tag="ACG842_002113"
     CDS             127..3654
                     /locus_tag="ACG842_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HFQ0248631.1"
                     /translation="MSLQFILGNGKKDHRQVLIDEASAWLDQDASNQVFFLVPNYSKF
                     EQEQEILAEMRRRSGKKTFSTTNVQVFSFYRLAWYLLQQTTLLTGNELSESGSAMILR
                     QILEKNEEELTIFRGEINKRGFIRQLQELYSELRTGNIQPEDLMLLFGDSPNAEDQQL
                     KMKDLKLIFSAYDSELTQRALTNEDALSILANYMQTQDFNNVKFIVSGFSRINGQEYQ
                     LLQAMMEKGQLVVDLLLDRPYTADLPEVLTLFHETGKLYFRLFQSARERKVPILVDRF
                     APEQTRPTELQLLQTMWEETSSQKKAAVHKIGGQEHLHLWEAENPTEEVRQLAVEIRR
                     LVSEENYRYRDIQVLTKNMSLYGNILRPIFQEMAIPFYIDEEQMMENHPLIEWINSLF
                     ALDRYSYRLSDIMRFFKTELFLSTEAETELDEWKQERDEFRKKLDITENVALAYNYQG
                     TYWTREKDWQFVAYDFEAEQIEDVQYLEEQSNTIRRLFQKAVPVFLKKIKKSKTGQEA
                     ANVFYQFLIQSGVQQQLLFWRNQEVDRGNLEAARNHEQTWGALIDLLDEYVVIYGESA
                     FDWSLFQEIITSGLENLSYGKIPTAIDQVRINRLELVRPNQAAVTFALGLNDQVFPDR
                     KETKGLLSSEEREVLNQALPDGQFLFDPSKESISFEPFQAYLVFLSGTDRLYLSYAQS
                     YDTDGSLKMSPYLRRITEYLSVPVERKEHLTIESDPNCYVGTYRSGINTVNRIYRLAL
                     DEKKPVPKHWQKLKELVLNSSWRNFALQIFESQEHLNVPVSMPEKMAEELYGKEIYTS
                     ISRMENFYNCEYKYFMQFGLKLKERTVYGLTPAATGDFYHESLDRFFKLLFSNQLSLV
                     NMSDAQRRDFTEKVLQEVFGELRFEILDSSARMNYIRYQLGQTIQKVTWALQKQSKNT
                     GMHPLQTEILFGQIAGAKGITGLELPLSNGGKLHVRGKIDRIDVASEQEDTWLSVVDY
                     KSSGRSFDVTEAYYGMAMQLLTYLDVALMDAVRLTGKAAVKPAGSYYLHVHNPVLTET
                     DDVEKNTLKKFSYDGVFVDDPALLEVLDHSLEAKEQSLIYPVKKNAKDEYQKGSFSKD
                     KFFTEEELQLFMAHNRENMRLAGDAITSGEIKLNPAYKDKQRIACEFCPFRSICTFDV
                     MLKENNYHRLEKIDKEEALRRILKRSEEE"
     gene            3651..7373
                     /gene="addA"
                     /locus_tag="ACG842_002114"
     CDS             3651..7373
                     /gene="addA"
                     /locus_tag="ACG842_002114"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0000724 - double-strand break repair via
                     homologous recombination [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HFQ0248632.1"
                     /translation="MNQIPLKPQNERFTDDQWQAIFDQGDNLLVSASAGSGKTTVLVR
                     RVIEKLKMGFDIDELLIVTFTEAAAREMKERIQEALQESVNSESDPVRRQHFTKQLVL
                     LPTANISTLHAFCLTVIRRYYYLIDIDPVFRMLTDETETILMKEDVWDELREALYAEN
                     DERFFQLTMNFSNDRSDDGLTNLVFSLYEFARANPDPQKWLEQLSDNYRLPEGLAKSR
                     LYQKQIRPLVLADIYQCVQLYEQMTQLAQGEGLEKMHEQVAGEQQQIKNIYEAFSQDR
                     LEEAYAGLEQLTFSTFKSSRKAELKEISNEVKGMRDKAKKLIQQISKSYFPVSPSQME
                     ELTDKALPLVEEMTKVTQSFMDGFSMRKREKGVLDFNDLEHLALQILTEKTKDVWLPS
                     EASKHYRKKFKEVMVDEYQDVNQLQEAILYWLREPDDTKGNMFMVGDVKQSIYSFRLA
                     DPSLFIGKYENFSKKEGGRRIVLAENFRSRKEVLSFTNLIFEQLMDPAVGQINYDEAA
                     KLIQGFSDFPENEQFEPEIMIYEKEQEESEIEIPTDDILEDKTEGELFMTGLKIRQLI
                     DSSFMIYDKKSKKSRPIEYKDIVLLTPTKKNNLTILEIFKTLDIPLEMNDAQNYFQAT
                     EIRTMISLLQLIDNPYQDIPLAAVLRSPIVGLIEPELASIRLANRAHTYYDAVLAYQA
                     SNEDELAAKLEHFGKQLEHWRELARRSSITDLLWDIYYETGYLEYVVGLPAGAQRQAN
                     LYALVDRAKAYEQSSFRGLYQFVRFIEKMQEKDKDLAEPVISIEDNAVRVMTIHASKG
                     LEFPVVFLLDMTKEFNLQDLRNRYAFEEKLGAGIRYMDPETRVLYDTLPFQAIKLAKQ
                     NKLLSEEMRKLYVGLTRAEQKLFIVGSYKNKEQMIQTWSEAADHEELVFDPALRLKGR
                     SSLMNWIGYGLIRHPEMQKYLEEEISTSLLQHSNAQFSISWMNQQSIIEQRQLLAEKE
                     LVNLDQQMKEDETLLADSLQKRLAYEYPYQASSQTTSYQSVSEIKRLFEDPDDTQESR
                     LTLESSQNKAASRQFRYTQEQLAEPKFLQKDRQVSAAAVGTATHALLQLLPLEMPTTE
                     SIHQKLQELVRKRLVDEKVAKKVDVSSIIWFFQTELGQQLIANKENVKREQPFSMLLP
                     ADEVFQDYPNQEDELLIHGIVDGYLEEKDHLNIYDFKTDFILHPDDPAEIDAIVQKYQ
                     GQLRLYQQAMSEALNKPVENVFLILLRVKQIININK"
     gene            7442..7783
                     /locus_tag="ACG842_002115"
     CDS             7442..7783
                     /locus_tag="ACG842_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HFQ0248633.1"
                     /translation="MELTQTEFVLCPKFEKTFSILGKKWNGLIIDVLLENGPQRFVEL
                     AAKIPMVSDRVLVERLKELEKEGIVTRNFACSEKSRSEYLLTEKGADLQKAMRQIQVW
                     SEKWITEEECG"
     gene            8092..9135
                     /gene="pheS"
                     /locus_tag="ACG842_002116"
     CDS             8092..9135
                     /gene="pheS"
                     /locus_tag="ACG842_002116"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HFQ0248634.1"
                     /translation="MSLQAQLESLKEATLVKITAAQDLNELNQIRVETLGKKGPITEV
                     LRGMKDLSPEERPKVGSFANEIRDLLTEEIERKKQVLEEEALNAALAKETIDVTLPGR
                     KVKHGTRHIVTQIMEEIEDIFVGMGYQIVEGYEVESDHYNFERMNLPKDHPARDMQDT
                     FYISDEILIRTHTSPVQARTMEKHDFSKGALRMISPGKVFRRDTDDATHSHQFHQIEG
                     LVVDKNITMGDLKGTLEVVMKKMFGEDREIRLRPSYFPFTEPSVEVDVSCFKCGGAGC
                     NVCKYTGWIEILGAGMVHPNVLKMSGIDPEEYSGFAFGLGPDRVAMLRYGVNDIRNFY
                     QNDLRFLSQFKGE"
     gene            9140..11560
                     /gene="pheT"
                     /locus_tag="ACG842_002117"
     CDS             9140..11560
                     /gene="pheT"
                     /locus_tag="ACG842_002117"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HFQ0248635.1"
                     /translation="MLVSYKWLNRYVDLSNVTPKELADKMSVTGIEVEGVTVPEEGLK
                     KIVVGEVKECVPHPDSDHLSICQVDIGEDELSQIVCGAPNVKVGIKVIVALPNSRIAG
                     NVKIKKGKMRGQVSNGMICSLQEIGYSDSVIPKEYAEGIYYMPQEAVNGDPVFPYLDM
                     DDSIIELSITPNRADALSMRGVAYEVGAIYRQTPTFDDEKLVESSRPASDKIQVEVED
                     KEAVPAYQIRIIEGVRIQPSPQWLQNLLMNEGIRPINNVVDVTNYILLLFGQPLHAFD
                     YDKLKSKEIFVRRAKENETLVTLDGESRELTTDNLVITNGKEPVALAGVMGGLDSEIT
                     NETTTVALESALFDPISIRRTAKQFNLRSESSARFEKGINHATVGEASEVAAAMIVSL
                     AGGEVLQGAVKGSEIKAEDVSVKISLERINQYLGTSLVVKEVNEIFEALGFSYEEKEY
                     VYSVVVPPRRWDIQIEADIIEEVARIYGYDQLPSTLPSGETVAGSLSKEQETTRKIRT
                     IFEGSGLSEAISYALTTEEKSRQFVINESNITRLDWPMSEERSVLRMNLISGLLDDIS
                     YNTARKNTEIALYEIGRVFFQENDPRTHLPQEVKHAAIAISGIWEEKDWQTKAQNVDF
                     FTIKGLLENLFEQLGITEDVHYQTVTEMKEMHPGRTAAIYLGEKFIGFVGQVHPTITK
                     SYDIPETYVAEFDLSAIVEAAQKGIVFEAVTKFPAVSRDIALLVKETVTNQELTEVIR
                     SAAGRFLTDIQLFDVYQGENIEKGHKSMAYSLTFVNPEATLTDEEINKAMEKVEKALV
                     ENLEASIR"
     gene            complement(11730..12740)
                     /gene="trpS"
                     /locus_tag="ACG842_002118"
     CDS             complement(11730..12740)
                     /gene="trpS"
                     /locus_tag="ACG842_002118"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HFQ0248636.1"
                     /translation="MKNIILTGDRPTGKLHLGHYVGSLKTRVAMQADEDNQLFVMIAD
                     MQALTDNAKNPEKVSSNVLQVALDYLAVGLDPAKSTLFIQSQIPELAELTMYYLNLVS
                     VGRVRRNPTVKTEIEQKKFGESVPTGFFIYPVSQAADITAFKANLVPVGEDQKPMLEQ
                     TQEIVQSFNHTYGEVLVEPKGVFPPKGMGRLPGIDGNGKMSKSLGNGIYISDPADVLQ
                     KKVMSMYTDPNHIHVQDPGQVEGNMVFTYLDVFGTDKEAIEEMKAHYRRGGLGDVKIK
                     RYLIDVLEAEFAPIRARREELEKDPSAVMEILRKGSEEAAKVAAQTLSEVKSAMGINY
                     FH"
BASE COUNT         4387 a         2244 c         2738 g         3541 t
ORIGIN      
        1 cgtatactta aaaaagtaga aatatcagaa ccttaaaagg atataatttg aagaatatag
       61 aacaaatgtt cagtattttt tgatataata aaggagcacc gtatcatgtg ttgaaggagg
      121 aaaagcatgt ctttacaatt tattcttgga aacgggaaaa aagatcaccg tcaagtcttg
      181 atcgacgaag catccgcttg gctcgaccag gatgcaagta accaagtatt tttccttgtt
      241 cccaactaca gcaaattcga acaggaacaa gaaattttag cggagatgcg aagaagaagc
      301 ggaaaaaaaa ctttcagcac aacaaacgtt caagtattta gtttttatcg tctggcttgg
      361 tatttgctgc aacagacaac tcttcttaca ggaaatgaat tatcagaatc aggatctgca
      421 atgatcttgc gacagatttt agaaaaaaat gaggaagaat taacaatctt tcgcggagaa
      481 atcaataaaa gagggtttat tcgtcaatta caagaactct acagtgaatt gcgtacagga
      541 aatattcaac cagaagacct gatgctttta tttggtgatt cacctaatgc agaagaccag
      601 caacttaaaa tgaaagactt gaagctgatt ttttcagctt atgacagcga attgactcaa
      661 cgtgcactaa caaatgaaga tgcactatct atactagcaa attatatgca gacacaagat
      721 ttcaacaacg tcaaatttat tgtcagtggt ttttctcgaa tcaatggtca agagtatcaa
      781 ttactgcagg caatgatgga aaaggggcaa ttggttgtcg atttattgct ggatcgtcct
      841 tatacagcgg atcttccaga agtacttacg ctctttcatg agactggaaa gctttatttc
      901 cgtctttttc aaagtgcaag agaacggaaa gtccctatat tagtggaccg atttgcacca
      961 gagcagactc gtccgacaga gcttcaactg ctgcaaacaa tgtgggaaga gacatctagc
     1021 caaaagaaag cggcagtaca caaaataggg ggacaagagc atctccattt gtgggaagct
     1081 gaaaatccaa ctgaagaagt ccgacaatta gctgttgaaa tcagacgact agtcagtgaa
     1141 gaaaactacc gttatcgtga tatccaagtg ctgacaaaaa atatgtcttt atatggaaat
     1201 atccttcggc cgatctttca agaaatggca attccttttt atatcgatga agaacaaatg
     1261 atggaaaatc atccgctgat cgaatggatc aacagtctgt tcgctcttga ccgctattct
     1321 tatcggctaa gtgatattat gcgttttttc aaaactgaat tatttctgtc gacggaagca
     1381 gaaaccgaac ttgatgaatg gaaacaagag cgagatgaat tcaggaaaaa actggacatc
     1441 actgaaaatg tagcacttgc ttacaattat caaggaactt attggactcg tgaaaaagac
     1501 tggcagtttg tggcgtatga ttttgaagca gaacagattg aagacgtaca gtatttggaa
     1561 gaacagtcaa atacgattag acgattattt cagaaagctg taccagtatt tttgaaaaaa
     1621 ataaagaaaa gcaaaacagg acaagaggcc gcaaatgttt tttatcaatt tttgatccaa
     1681 agtggcgtac agcaacaatt attgttttgg cgtaatcagg aagtagacag aggaaactta
     1741 gaagcggcaa gaaaccatga gcagacatgg ggagcgctga ttgatctgtt ggatgaatat
     1801 gtagtaattt atggtgaatc ggcttttgac tggtcactgt tccaagaaat cattacaagt
     1861 ggtctagaaa atctaagtta tggtaaaatc cctaccgcaa tcgaccaagt gcgaatcaat
     1921 cgtttggaat tagttcgacc aaatcaagct gccgtcactt ttgcattagg gttgaatgat
     1981 caagtgtttc cagaccgaaa agagacaaaa ggattgttgt ccagtgaaga acgagaagta
     2041 ttaaatcagg ccttgccaga tggacagttt ttattcgacc catcaaagga aagtatctct
     2101 tttgagcctt tccaagccta tcttgttttt ctttccggaa cagatcgatt atatcttagt
     2161 tatgcacaaa gctatgacac agatggttcg ctgaagatgt ctccttattt gcgtagaatc
     2221 acagaatatt tatctgttcc cgtcgagaga aaagagcatt tgacgatcga aagcgaccct
     2281 aattgttatg tagggactta cagaagcgga atcaatacag tcaataggat ttatcgatta
     2341 gctttagatg aaaaaaaacc tgtaccaaag cactggcaaa agctcaaaga attagtactg
     2401 aattcgtcat ggcgaaattt tgctttacag atttttgaaa gtcaggaaca tttaaatgtt
     2461 cctgtttcga tgccagaaaa aatggcggaa gagctttatg gaaaagagat ttatacttca
     2521 atttcacgta tggagaattt ttataattgt gaatacaaat acttcatgca atttggtttg
     2581 aaattgaaag aacgaactgt ttatggtcta actccagcag caactggtga tttttatcat
     2641 gaatcattgg atcgtttctt caagctactg ttttcaaacc aattatcctt agtgaatatg
     2701 tccgatgcgc agcgtagaga ctttactgaa aaagtgctgc aggaagtatt cggagaactt
     2761 cggtttgaga tattggatag ttctgcaaga atgaactata tccgctacca gttaggacag
     2821 acgattcaaa aagtgacatg ggctttgcaa aaacagagca aaaatacagg catgcatcct
     2881 cttcagacag agattctatt tggtcagatt gctggagcaa aaggtatcac aggtctggaa
     2941 ttaccgttga gtaatggtgg aaaactccat gttcgaggga aaatcgatcg aatcgatgta
     3001 gcttctgaac aagaagatac ttggctaagc gtcgttgatt acaaatctag cggacgttca
     3061 ttcgatgtca cagaagctta ttatggcatg gctatgcaat tattgacgta tctggacgta
     3121 gcattgatgg atgcggtacg tttgactgga aaggcggctg ttaaaccagc aggatcatat
     3181 tatttacatg ttcataatcc tgtattgacg gagacagatg atgtagagaa gaatacattg
     3241 aaaaaattta gttatgatgg cgtttttgtc gatgatcccg ctcttttaga agtactagat
     3301 cattcactgg aagcaaaaga acaatcactg atctatccag tgaaaaaaaa tgcgaaagat
     3361 gaataccaaa aaggtagttt cagtaaagac aaatttttca cggaagaaga actgcaacta
     3421 ttcatggcgc ataatcggga aaatatgcgg ttagctggag atgcgattac ttccggagaa
     3481 atcaaactaa atccagctta taaagataaa cagcggattg cctgcgaatt ttgtccattc
     3541 agaagcatct gtacgtttga tgtgatgctg aaagaaaata attaccaccg tctggagaaa
     3601 atagataagg aagaagcact gcgtagaata ttgaaaagga gtgaagaaga gtgaatcaaa
     3661 ttcctttaaa accgcaaaat gaacgattca cagatgatca gtggcaagcg atttttgatc
     3721 aaggagataa tctattagtc tctgcgtctg ccggatcagg taaaactaca gtgcttgttc
     3781 gaagagtgat tgaaaaacta aaaatgggtt ttgatatcga tgaattattg attgtgacat
     3841 ttactgaagc tgccgcaaga gaaatgaaag aaagaattca agaggcatta caagaatccg
     3901 tcaatagcga aagtgaccct gtacgtagac agcattttac taagcagtta gttttattgc
     3961 ccacagcgaa tatctcaacc ttacatgctt tttgcctgac agttattcga cgttattatt
     4021 acttaattga tattgatcca gtctttcgca tgttaacaga tgaaacagaa acgatcttga
     4081 tgaaggagga tgtgtgggac gagctgagag aagcacttta tgcagaaaat gatgaacgat
     4141 ttttccaatt aacaatgaat ttttctaatg atcgttcaga tgacggcttg acgaatctgg
     4201 tgttttctct ttatgagttt gcccgtgcca accctgaccc ccaaaaatgg ctagaacagt
     4261 taagcgataa ttatcgtttg cctgaggggc tagcgaaatc tcgattatat caaaaacaaa
     4321 tccgtccttt agttttggca gatatatacc aatgtgtaca gttatatgaa cagatgactc
     4381 agttagcaca aggtgaagga ttagaaaaaa tgcatgagca agtagctgga gaacaacagc
     4441 agatcaagaa tatctatgaa gcgttctcgc aagatcggtt agaagaagct tatgctggtt
     4501 tggaacaact aactttttca acttttaaga gcagtcggaa agcagaattg aaagaaattt
     4561 ccaatgaagt caagggcatg agggataaag caaaaaaact gattcagcag ataagtaaaa
     4621 gttattttcc tgtttcccca agccaaatgg aagaattaac ggacaaagca ctaccgttag
     4681 tcgaagaaat gacaaaagtc acgcaaagct tcatggacgg tttcagtatg cgtaaacggg
     4741 aaaaaggtgt gctggatttc aatgatctgg aacatttagc gttgcagatt ctcacagaaa
     4801 aaacgaaaga tgtatggctg ccaagtgaag cttcaaaaca ttatcgaaaa aaattcaaag
     4861 aagtaatggt agatgaatat caagacgtga accaattaca agaagccatt ttatactggc
     4921 taagagaacc cgatgataca aaaggaaata tgttcatggt tggggatgtc aaacaatcca
     4981 tctattcttt ccgtttagca gatcctagtt tatttatcgg gaaatacgag aatttctcaa
     5041 agaaagaagg cggacgtcga atcgttttgg ctgagaactt ccgctctaga aaagaagtcc
     5101 tttcttttac gaatcttatt tttgaacaac taatggatcc agcagtgggt caaatcaatt
     5161 atgacgaagc agccaaactg atccaaggat tttcagactt tccagagaat gaacagtttg
     5221 agccggaaat catgatctat gaaaaagaac aggaagaatc agagattgag attccgacgg
     5281 atgatatact ggaagacaaa acagaaggcg aactttttat gacaggacta aaaattcgtc
     5341 aattaattga ttcctccttt atgatttatg ataaaaaatc aaaaaaaagt cgtccgatag
     5401 agtacaaaga cattgttctc ttgacaccta caaagaagaa caacttaacg attttagaga
     5461 ttttcaaaac attggatatt ccattagaga tgaatgatgc tcaaaactat tttcaagcga
     5521 cagaaattcg cacgatgatc tcgctgctac aattaatcga taatccctat caagatattc
     5581 cgcttgcggc tgtattgcgc tcaccgatcg tgggtctgat tgaaccagaa ttagccagca
     5641 ttcgattagc taaccgtgca catacttact atgatgctgt gcttgcttat caagcatcaa
     5701 acgaagatga attagcagct aagctggaac attttggcaa gcaactagag cattggcgag
     5761 aacttgctcg ccgttcttcg atcactgact tgttatggga tatctattat gagacgggat
     5821 atctagagta tgtggtcggt ctgccagcag gggcacagag acaagccaat ctttatgcat
     5881 tggttgatcg tgccaaagct tacgagcaaa gttctttcag aggattatac cagttcgtgc
     5941 gtttcattga aaaaatgcaa gaaaaagata aagacttagc agaaccagtc atttcaatag
     6001 aagataatgc tgttcgcgtt atgacgatcc atgcaagtaa aggcttggaa ttcccagttg
     6061 tatttttgtt agatatgaca aaggagttca acttacaaga cttgcgcaat cggtatgctt
     6121 ttgaagaaaa acttggagca ggaattcgct atatggatcc tgaaacacga gtgttatatg
     6181 atacgttacc gtttcaagcc atcaaattgg caaagcaaaa caaattgttg tcggaagaaa
     6241 tgcgtaaatt gtatgtagga ttgactcggg cagaacaaaa gcttttcatt gtcggttcct
     6301 ataaaaataa agagcaaatg atccaaacgt ggtctgaagc agctgatcat gaggagttag
     6361 tttttgatcc tgcgctacgt ttgaagggca gaagcagctt gatgaattgg attggttacg
     6421 gattgatccg tcatcctgaa atgcagaaat acttagagga agaaatttcg acttctcttc
     6481 ttcaacattc aaatgcacag ttttctattt catggatgaa tcagcaatcg atcattgaac
     6541 aaagacagtt gcttgctgaa aaagaactgg ttaacttaga tcagcagatg aaagaagatg
     6601 aaactttatt agcagattcg ttacaaaaac gtctggcata tgaatatcct tatcaagctt
     6661 cttcacaaac cacaagttat caatctgtat ctgaaatcaa acgattattc gaagatccag
     6721 atgatacaca agaatcacgt ttgactctgg aaagcagtca gaacaaagca gcatcccgtc
     6781 aatttcgtta tacgcaagaa caactagcag aaccaaaatt tttgcaaaaa gatcggcaag
     6841 tgtcagcagc tgctgttgga actgctacgc atgctttgtt acaattactg ccattagaga
     6901 tgccaactac agagagtatc catcaaaaat tacaggaatt ggtaaggaaa cggttagtag
     6961 acgaaaaagt cgcgaaaaaa gtagatgttt cttctattat atggttcttt caaacagaac
     7021 ttggtcaaca gctgattgcc aacaaagaaa acgtaaaacg agaacaacct ttttccatgc
     7081 tgcttcctgc tgacgaagta tttcaagact atccaaatca agaagatgaa ctattaatcc
     7141 acggaatcgt cgatggatac ctagaagaaa aagatcatct caatatctat gattttaaaa
     7201 cagattttat tttacatccg gatgatccag cagaaataga tgcaatcgtc caaaaatatc
     7261 aaggacagct aagactttac caacaagcaa tgtcagaagc tttgaataag ccagtagaaa
     7321 atgttttcct aattttgctt cgagtgaaac aaattataaa cattaacaaa taattataat
     7381 tgagcaaact tgacttatgt tttgtaagga ttatgctata ctctgggaag aggtgagtat
     7441 gatggagcta acacagacag aattcgtttt atgccctaaa tttgaaaaaa cattctctat
     7501 tttaggaaaa aaatggaacg gattgattat cgatgtacta ctcgaaaacg gtccgcaacg
     7561 ctttgttgag cttgcggcaa aaatcccgat ggtcagtgat cgagtattag tcgaacgctt
     7621 gaaggaatta gaaaaagaag gaatagtcac tcgaaacttt gcctgtagcg aaaagagccg
     7681 ttcagaatat ctcttgactg aaaaaggagc agacttgcaa aaagcgatgc gtcaaataca
     7741 agtttggagc gaaaaatgga tcacagagga agaatgcggt tgattttgta aaaaaaatct
     7801 tgtaaactat agtccatacg aataagtaaa gaacgatgac gaaaaagagt agtttggcta
     7861 aaccagttca gggagatctg tcgcagactg aaagcagatc ctgtgtttga aaaatgaagt
     7921 tcactttccg agttcgtctg ataaaatcag accggcaatt gtcgttaacg aagtaagagt
     7981 gcggtgtaag tcatttatcc gcaaattagg atggtaacgc gaagacctcg ttccttaaca
     8041 ggaatgaggt ctttttgttt tgtatttgga atttaaagga ggaacaagca catgtctcta
     8101 caagcacaat tagagagctt gaaagaagca acgctcgtca aaatcacagc ggcacaagat
     8161 ttaaatgaat taaatcaaat acgtgttgaa acgttaggaa aaaaaggacc gatcacagaa
     8221 gtattacgtg ggatgaaaga tctaagtcca gaagagcgac caaaagtcgg aagtttcgca
     8281 aatgagatcc gagatctttt gacagaagag atcgagcgaa aaaaacaagt attagaagaa
     8341 gaagcgttaa atgcagcttt ggcaaaagaa acaatagacg tgacactacc tggtagaaaa
     8401 gtaaaacatg gtacacgtca tatcgtgaca caaatcatgg aagaaattga agatattttt
     8461 gtgggtatgg gctatcagat cgtagaagga tatgaagtag aatctgatca ttacaatttc
     8521 gaacggatga atctgccaaa agatcatcca gcaagggaca tgcaagatac tttctatatt
     8581 tcagacgaga tattgattcg gacacatact tcaccagtcc aagcacggac aatggaaaaa
     8641 catgatttct ctaagggtgc tctacgaatg atctcgccag ggaaagtctt ccgcagagat
     8701 acagatgatg cgacccacag tcatcagttc catcaaattg aagggctagt tgttgataaa
     8761 aacatcacaa tgggcgacct taaaggaaca ttagaagtcg taatgaaaaa aatgtttggg
     8821 gaagatcgtg aaattcgttt gcgtccaagt tatttcccat ttacggaacc atcagtagaa
     8881 gtagatgtca gctgtttcaa atgtggtggt gccggttgta acgtatgtaa atacactgga
     8941 tggatcgaaa ttttaggagc tggcatggta cacccgaatg tgttgaagat gtcaggaatc
     9001 gatccagaag aatattcagg ctttgctttc ggccttggac cagaccgcgt agctatgttg
     9061 cggtatggag tgaatgatat ccgtaatttc tatcaaaatg acttacgttt cttaagtcag
     9121 ttcaaggggg agtaagaaaa tgttagtttc atataaatgg ctcaatcgtt atgtagattt
     9181 atcgaatgta acaccaaaag aattagcgga taagatgtct gtgacaggaa tcgaagtaga
     9241 gggagtaacg gttccagaag aaggcttgaa aaaaatcgtt gtcggtgaag tcaaggaatg
     9301 cgtaccacat ccagattctg atcacctttc tatttgtcaa gtcgatattg gcgaagacga
     9361 actgtctcaa atcgtttgtg gagcgcctaa tgtgaaggtt ggaatcaaag tgattgttgc
     9421 acttcctaac tcacgtatcg ctggtaatgt aaaaatcaaa aaaggaaaaa tgcgtggcca
     9481 agtatcaaat ggcatgattt gctcattaca agaaattggc tattcagata gtgttatacc
     9541 gaaagaatac gctgaaggta tctattatat gccacaagaa gctgtaaatg gcgatccagt
     9601 ttttccttat ttggatatgg atgattcgat tattgaatta tctatcacac cgaatcgtgc
     9661 ggatgcattg tcaatgcgtg gcgtagctta tgaagttggg gcaatttatc gtcagactcc
     9721 tacattcgat gacgaaaagc tggtagaatc ctcacgtcct gctagcgata aaattcaagt
     9781 ggaagttgaa gataaagaag cagttcctgc ttatcaaatc agaatcattg aaggagtaag
     9841 aatccagcca agtcctcaat ggctgcaaaa tcttttgatg aacgaaggca ttcgtccaat
     9901 caacaatgta gtagatgtga cgaactatat tttgctttta ttcggacaac cactacatgc
     9961 atttgactac gacaaattga aatcaaaaga gatctttgtc cgccgtgcaa aagaaaatga
    10021 aacattagta acactagatg gagaatctcg ggaattgaca actgacaatc tcgtcattac
    10081 taacggcaaa gaaccagttg ctttagctgg tgtcatggga gggttagact cagagatcac
    10141 gaatgaaaca acaacagttg cactagaatc tgctttattt gatccaattt cgattcgtcg
    10201 tacagcaaaa caatttaatt tgcgtagtga atcctctgca cgttttgaaa aaggaatcaa
    10261 ccatgcgact gttggagaag ccagtgaggt tgctgcagca atgatcgttt ctcttgcggg
    10321 tggggaagtt ctacaaggcg cggtcaaagg ctcagagatc aaagcagaag acgtatcagt
    10381 gaagatttca cttgaacgca tcaatcaata tttaggaaca tctcttgtcg ttaaagaagt
    10441 aaatgagatt tttgaagcac ttggatttag ctatgaagaa aaggagtatg tttattccgt
    10501 agtagtacca ccaagacgct gggatatcca aatcgaagca gatattatcg aagaagttgc
    10561 tcgtatttat ggatacgacc agttgccatc tacacttcca agtggagaaa cggttgctgg
    10621 aagcttgtca aaagaacaag aaactactcg taaaattcgt acaatctttg aaggaagcgg
    10681 attgagcgaa gcaatcagtt atgcattgac aacagaagaa aaatcaagac agtttgttat
    10741 aaatgaaagt aatattacac gtttggactg gccaatgagc gaagaacgtt ctgtattacg
    10801 aatgaatttg atcagcggac tattagatga tatcagttat aacactgccc gaaaaaatac
    10861 agagattgct ttatatgaaa ttggtcgggt gttcttccaa gaaaatgatc cacgtacaca
    10921 tctgccgcaa gaagtaaaac atgcagcaat cgctatttct ggtatttggg aagaaaaaga
    10981 ttggcagacg aaagctcaaa atgtagactt tttcaccatc aagggtcttt tagaaaactt
    11041 atttgaacaa ctaggcatca ctgaagatgt ccattatcaa acagtgacag aaatgaagga
    11101 aatgcatcct ggaagaactg ctgcgattta ccttggcgaa aaatttattg gttttgtcgg
    11161 tcaagtgcat ccaacgatta caaaaagtta tgatattcca gaaacatatg ttgctgagtt
    11221 tgatttatct gctatagtgg aagctgctca aaaagggatc gttttcgaag cagtgacaaa
    11281 attcccagca gtcagccggg acatcgcttt gcttgtaaaa gaaactgtga caaatcagga
    11341 attaacagaa gttatccgtt ctgctgccgg aagattttta acagatatcc aattatttga
    11401 tgtctatcaa ggagaaaaca ttgaaaaagg acataaatcc atggcgtata gcctgacatt
    11461 cgtcaaccca gaagcaacat tgacagatga agaaatcaat aaagcaatgg aaaaagtcga
    11521 aaaagcgttg gtagaaaacc tagaggcaag tattcgctaa gaaggaaaat atagtaagta
    11581 acgatcaagt agcttttgaa gaccatacat ttgaaaaaaa gatgtgacac aacttcgtca
    11641 catctctttt tttgatttta aaaaaggaat agcacagaga cgagataaaa aaacaggcct
    11701 ttacagcctg cttttttaga gtaaatgaat taatggaagt aattgattcc cattgctgat
    11761 tttacttcag aaagtgtttg tgcagctact ttagcagctt cttcactacc tttacgtaag
    11821 atttccataa cagctgaagg atctttttcc aattcttctc ggcgcgcacg gatcggtgca
    11881 aattcagctt ccaatacatc aatgaggtat cgtttgatct ttacatcacc aagtccgccg
    11941 cggcggtaat gagctttcat ttcttcaatt gcttctttat ctgttccgaa tacatccaag
    12001 taggtgaaga ccatattccc ttctacttgt ccaggatctt gtacatggat gtggtttgga
    12061 tcagtataca tactcatgac ttttttctgt aagacatcgg caggatcgga gatataaata
    12121 ccgtttccta acgatttact cattttgcca tttccatcga ttccaggtaa tctacccatt
    12181 ccttttggtg ggaatacgcc ttttggttcc accaagactt caccatatgt atgattgaag
    12241 ctttgtacga tttcctgtgt ctgctctagc attggttttt gatcctcacc aacaggtaca
    12301 agattcgctt tgaatgcagt gatatctgct gcttgagaaa caggatagat aaagaatcct
    12361 gtaggcacgc tttcaccgaa ttttttttgt tcgatttctg ttttgacagt cggattgcgc
    12421 cgaacgcgtc ctacgctgac taagttcaaa taatacatgg ttaattcagc taattccgga
    12481 atttgtgatt ggataaataa agtagatttt gcaggatcta atcccacggc taaatagtct
    12541 aaagcaactt gcaagacatt tgatgaaacc ttctcaggat ttttagcatt atctgtcagt
    12601 gcttgcatgt cagcaatcat gacaaacagt tggttgtctt catctgcctg catagccact
    12661 cgggttttta gagaaccgac gtaatgccct aaatgcagct tcccagttgg tcgatctccc
    12721 gttaaaataa tatttttcaa aaaagacacc tctttacgat ttgtaagtct gagcacttaa
    12781 tatcttaccg tttttcctga aaagaaacaa cagttcctaa ggaaaaaaca aggaaagagg
    12841 ataaaataac taatagatta gaaatggaag aaatatttat gggagaataa acgttctaag
    12901 aaactttaca
//
LOCUS       DAZKLM010000080        12809 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3764
            SAMEA115706913-rid22582563.denovo.083, whole genome shotgun
            sequence.
ACCESSION   DAZKLM010000080 DAZKLM010000000
VERSION     DAZKLM010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706913
            Sequence Read Archive: ERR13196647
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12809)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12809)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287823.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287823.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,004
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,849
            CDSs (with protein)               :: 2,849
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 41 of 96
            Pseudo Genes (incomplete)         :: 52 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 15 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12809
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706913-rid22582563.denovo.083"
                     /isolate="VRE3764"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..177)
                     /locus_tag="ACG842_002119"
     CDS             complement(<1..177)
                     /locus_tag="ACG842_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFQ0248637.1"
                     /translation="MKKTDRRVKKTEKALAETLSTLLVNKKIQAITIRELTETADVHR
                     STFYTHYKDIYDLYD"
     gene            319..933
                     /locus_tag="ACG842_002120"
     CDS             319..933
                     /locus_tag="ACG842_002120"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0248638.1"
                     /translation="MKLTFKPLEKKDYKKAIYFAVEGMNFERYMENELLLNLYGRYFL
                     YMELNRATQILALYAEDELAGVLLAEIKGEKKKHRSFGQQIYVKIFDFLQNIFAKESK
                     GAYETANEQMLAAYLKKHSPDGEIIFLAANPDLKTKGIGTKLLTEFEKREKGKEIFLF
                     TDSGCTYQFYERRGFERLKEKISKIKILDKEIDLRCMLYRKIIQ"
     gene            1122..3002
                     /locus_tag="ACG842_002121"
     CDS             1122..3002
                     /locus_tag="ACG842_002121"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFQ0248639.1"
                     /translation="MKLQKWIQEHRNHITALSAVLIIFGFIGKFTGFVLFYNIALAIA
                     SIIAVIPIMIHAYQAVKVKVISIDLLVTIAVLGAFMIGEYKESAIVTFLFLFGSYLEQ
                     KTLTKTRESIKALTEMAPSTASVVQEDGTTEEVEIDDVEEGDIVLVKTGGAIPVDGRV
                     IDGKGYADEAAVTGESRQVVKISGDEVFSGTILADGYVTIEATKVGDDTTFAKIIELV
                     EEAQDTKSHAEKFIDRFSQYYTPTVLLIALVVGLISRDFRLAITVLVLGCPGALVIGA
                     PVSNVAGIGNGAKRGILVKGGEAMDTFSKIDTFVFDKTGTLTKGTTAVSTIKNYTEDE
                     GDSLALVARLETLSDHPLGRAVIHYAQEKDIDFQQLAVKDNQTIKGQGITAEIDGHTV
                     CAGNTKLIAAHKLSLTPKQVQDLHQLQQTGSSVIIVAIDKKITQIIGVSDVIRPEVAE
                     QLAKLKQAGAKQLVMLTGDNQMTADYVADMLGIDEVHAELLPDEKVQFVKKYQEQGLR
                     VAFIGDGINDSPSLAAADIGIAMGSGTDVAIETSDVVLMQSNFESLVHAYRLAKKTVL
                     NTRENVVIAIGVVLFLLNGLFAGFIYMASGMFVHEASILIVIFNAMRLLYFGHESKEE
                     KS"
     gene            3081..3308
                     /locus_tag="ACG842_002122"
     CDS             3081..3308
                     /locus_tag="ACG842_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HFQ0248640.1"
                     /translation="MKKIIMQLDTLACPSCMQKIEHAVSQQPGVSNTKVLFNASKIKA
                     NFDPDEVHPEQLAAVVEKLGYEVQSAKVKEA"
     gene            3313..3855
                     /locus_tag="ACG842_002123"
     CDS             3313..3855
                     /locus_tag="ACG842_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HFQ0248641.1"
                     /translation="MNIVEEKFVQETKQADIDHHKPTAGAMVGHVLANFKLEEMKLIQ
                     AQLYVLGTSSQELSTLFSEYADQEARWFNKISHELVIENEIVPSTLEEVMRYGKIYEH
                     GQNKYLPASTMLENFAKDFDFQNVFITRAIKLAEKEEKYGLQQLLVELLSFNKEIIYQ
                     VQRLQGKTVRQDLDEEDDDD"
     gene            4082..4798
                     /locus_tag="ACG842_002124"
     CDS             4082..4798
                     /locus_tag="ACG842_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
                     /protein_id="HFQ0248642.1"
                     /translation="MKKRILVTIGLAFLLAGCTNGNESKKESTKETAKATVATTTTTS
                     TESKSTAASTETADTSTSSESSKTTETTQTSTSSSSVEASSSSSESSEQVEQTLWNAD
                     KSAQLEAFMTKWGQTMGQQYKSYTNQMSVDLYGLKVPQAILNGEWKMAIGGVPVSAEW
                     SESGTGQADYQITAVYSDAETEPYLKKHVYLFGFQQNQPKVLVTQQNQGNPDNYLYFN
                     ETANNELKNGFNQIVYGQAN"
     gene            complement(4858..6693)
                     /locus_tag="ACG842_002125"
     CDS             complement(4858..6693)
                     /locus_tag="ACG842_002125"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HFQ0248643.1"
                     /translation="MQIFVLEPKEGWKETIQVALKIFTQRQAPMILTIIIIMGFFFLL
                     LLISNRFWYALTATLIINLLLTISTVIKMEMREEPVFPSDLKMLTGLSELLSMVSPVL
                     LIVGGLILLLLLITSIIVQRRLQKQYSLKIHWKRRFISIAVLLGCFSGVFFINHKNSP
                     SFLLFNLFKVNKTFFNQQGAVKDNGPVIQFLNNIDIKIMEEPAGYSKEKINGIMKKYN
                     TKADEINQTRSDWLNNQTIVLNLSESFSDPARVPNLTVPTDPIPYIKSIITEGTGGLM
                     LSVGYGGGTANMEWEGLTGLSISNLSASLVTPYTQLVERQKISPNITDLFDEKIAIHP
                     FTAALYRRKQVFDKFGFDRFYYLDSPDKLTYTDKIEQSPRISDDSAYKETLKALQSNT
                     ETSQFIQLSTMQNHMPYDDYYSELNYTAEGTAVIPNRRKELQTYMQGLHYTDTAVKKF
                     IQEIDKIDKPITFVFYGDHLPAIYSGNDSKKYGLEQHQTDYFIYNNKYSREQAATLNK
                     EVVAPNNFGAMALEHANIKVTPYYALLTEVSNHLPALTIDPTESLSNRFNGKQVFVTE
                     DSQIIQEKNLTEKQKEILEDYRLIQYDLVAGNQYSATWATQKIKD"
     gene            complement(6889..7887)
                     /locus_tag="ACG842_002126"
     CDS             complement(6889..7887)
                     /locus_tag="ACG842_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0248644.1"
                     /translation="MFLSNFFVRCLFLKKIKKESYGFLTIFVACIMMVLQRILISAVT
                     IPTYATNPGRFYIYTFLQTSVVIGANLIPLYMGYQYQKIARLKVLHYLSRFAFIFLIA
                     TLVCNIFFYVQAGSLNIRDYWLILTPISQNYFTYAVSCTLLFCSIPYTVGFLNKLSKT
                     TIKYLLLFLTIGLVGCSTLFNKDPFALQNGQSILWIFYLFLMGYGLKEWNWKEKVRYK
                     LPQVFISLIVLFGLIILMTQISLIIRGNTDTALRFSKPYSLFAMYYTFSSFLFLEMLS
                     DKLGIKGRGNFLAVFLIVTQVICNFPLVSYRVSTFMKREFPNSGKAWLINIGQFFI"
     gene            8106..9326
                     /gene="sstT"
                     /locus_tag="ACG842_002127"
     CDS             8106..9326
                     /gene="sstT"
                     /locus_tag="ACG842_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0015826 - threonine transport [Evidence
                     IEA];
                     GO_process: GO:0032329 - serine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HFQ0248645.1"
                     /translation="MSLVQRIVVGILVGILFGIFFPTWTFISILGTLFVSCLKAIAPI
                     LVFFLTMASIAKHKIGNKTFVKPILILYLVGTLLSALVAVIVSSIFTVPITLQETVSE
                     KAPQSLESVLSTMLTNVTQNPVQSLIDANYLGVLFWAVLLGLAFRARSESTKELVDQI
                     SIALSQVVQVIIAFAPIGILGLVYQSIATTGIAGLAEYLQLLLVLIGTMLFVALVVYP
                     FLTFLFIKENPYPLIFFCLKESALPAFFTRSSAANIPINMMLAERLKLTKESYSISIP
                     LGATINMGGAAITISLMTLTAVHSLGIEAPFVLKVLLCILSALAACGASGVAGGSLLL
                     IPLACSLFGISNDVAMQIVGIGFIIGVIQDSMETALNSSSDLLFTAIGELGARKRSGE
                     KIKLKDCLDGLSER"
     gene            9473..10108
                     /locus_tag="ACG842_002128"
     CDS             9473..10108
                     /locus_tag="ACG842_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HFQ0248646.1"
                     /translation="MDFLRKFGFFFLLLGVLSDFLTPYVLGLYYPELDQMKSVISVFG
                     EVSSPVRKAFLIWSVVSGIFFILSLPALYAAFVSTSRVLATLVTAAIAFYGIGDCIFT
                     GLFSINTHESSWNVSTWVHNIGSGLGYSGFLLFPVLLFLIYRKNGQMDVSHLYLGLSV
                     ISLLFAAIYGLARIPSINSWPLLNKIGFCQRISFFFNYVPIAWLSILHLKQ"
     gene            10299..11039
                     /gene="truA"
                     /locus_tag="ACG842_002129"
     CDS             10299..11039
                     /gene="truA"
                     /locus_tag="ACG842_002129"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HFQ0248647.1"
                     /translation="MRNIKLTIEYDGKRYSGWQRLGDDDKTIQGKIEKVLHQMTNEEI
                     EIIGSGRTDAGTHARGQVANFKTNTEIGLAEMIDFMNRYLPRDIVIKRIEEMPERFHS
                     RYNAVGKKYSYYVWNNVIPSAFERNHSFYFPQELDMDKLNAACEKLVGTHDFIGFSAL
                     KKSKKSTVRTIDELSIERKGEMLHFTFVGDGFLYKMVRIIMGTLLEIGTGAMPIETID
                     EIFESKVRQHAGETVPAQGLFLDEVYYR"
     gene            complement(11184..>12809)
                     /locus_tag="ACG842_002130"
     CDS             complement(11184..>12809)
                     /locus_tag="ACG842_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0248648.1"
                     /translation="KNQIIDIIFMLFLSFAYVIPLFMSKGIYHSVNQDTYFHLSRIIG
                     LDNVWSSPVNFNNFDHHGTMMNIFYPWLTLYPAFLFYKMMGNLVLSYNIYYFFITFLT
                     MVVSYFSMKQIKNNRYISLLFSIIYTFSAYRAIDIFHRASLGEAVALTFLPLILMGCY
                     EIYIRDYQKWYWLSIGMTLVVYTHLLSVAMVSVFIGGTLFLSFYFWDQKIARLLSLLK
                     ATVLTFFLSAGFLIPFIQQSRAQELKVPLGKELSGMAPSDMLTHILNNNYNNYTIGLF
                     LFLGLIGAFIFIKKLTADDLFIFFLGVFVLFCSTNLFPWQLFNHTPVKSLQFVWRLNG
                     FSSLFIAYTMSIVIYYIFSTKNNSWKIMVFTFLALILHLSGVSNSIHANKDSLTLIAP
                     EDAVAIAENYNHTDYANKESIYHPDMINNDQYLLGNQEINPTTHFMSNKLTIELDNSN
                     NKNTVLTTPIYRYKGQVASINGKLVQTKLSKFGTTELTIPPGINKVVITYQYTKLAIA
                     SRYLSIVTLILFLLYRFTFSKQKQPRREVQHSH"
BASE COUNT         4284 a         2034 c         2508 g         3983 t
ORIGIN      
        1 atcatataga tcatagatat ccttataatg tgtataaaaa gtcgaacgat gtacatctgc
       61 cgtttctgtt agttcgcgta tagtgattgc ttgaatcttt ttatttacta ataatgtgga
      121 tagtgtttca gccaaagctt tttctgtttt tttgacgcgt ctatctgtct ttttcattta
      181 cctatctcct tatccgacat atcaaagcaa agctgtcgct taatcgacaa acttatagat
      241 gattgattat agatagtaga cactgtgtct attataatgt aagtataaca caatcattga
      301 agttgaggag gcgaaatgat gaaactaact tttaaaccct tagaaaagaa agattacaaa
      361 aaagctatct attttgctgt tgaaggaatg aactttgaga gatatatgga aaatgaactt
      421 ctgctgaatc tttacggaag atattttttg tatatggagt tgaatcgagc aactcaaatt
      481 cttgctttgt atgcagagga cgagttggca ggtgttttgc tggcggagat aaaaggagaa
      541 aaaaagaaac atcgttcttt tggacagcaa atatatgtta agatattcga ttttttacaa
      601 aatatatttg ctaaagaaag taaaggagct tacgagacag caaatgaaca aatgcttgca
      661 gcatatttaa agaagcattc gccagatgga gagatcattt ttttagctgc aaatcctgat
      721 ttgaaaacaa aaggcatcgg taccaagttg ctaacagaat ttgaaaaaag agagaaagga
      781 aaagaaatat tcctttttac agatagtggc tgtacgtatc aattctatga acgacgtgga
      841 tttgaaagac taaaggaaaa aattagcaag ataaaaatct tagataaaga aatagatctc
      901 cgatgtatgc tgtacaggaa aatcattcaa tgaaaatttc gatagaaacg acaatagttg
      961 gcaagatcac ttttagtgag tcttcttgct aactattttt tgtagtgtat tctaccaaaa
     1021 gaaaaccgcc tttttgacta aacttgatag tggtcaattt tttatgatgg ataagtatct
     1081 atacttagtc tcgtaagaat gaaaggaaaa gagggagaaa gatgaaatta caaaagtgga
     1141 tccaagagca tcgaaatcat atcaccgcct tatcagctgt gttgattatt tttggtttta
     1201 tcggaaaatt tactggtttt gtcttgttct ataacattgc tctagccata gcttctatta
     1261 ttgcggtcat acctattatg atccatgcgt atcaggcagt gaaagtcaaa gttatctcaa
     1321 ttgatttact cgttactatt gctgtgcttg gtgcctttat gatcggagaa tacaaggagt
     1381 cagctatcgt tacattcttg tttttgtttg gtagctatct ggaacaaaaa acattgacca
     1441 aaacgcggga gtcaatcaag gctttgaccg agatggcccc atctactgct agtgttgtcc
     1501 aagaagatgg aacgactgaa gaagtggaaa tcgatgacgt ggaagaaggc gatatcgtct
     1561 tagtaaaaac tggtggagct attcctgtgg acggacgagt cattgacggg aaagggtatg
     1621 cggatgaagc cgcagtgact ggtgaatcac gacaagtcgt caaaatcagt ggagatgaag
     1681 tcttctctgg aacgatttta gcagatggct acgtgacgat tgaagcaact aaggtcggag
     1741 atgatacgac ctttgccaaa attatcgaat tagtagaaga agcacaagat accaaatccc
     1801 atgcggaaaa attcattgat cgattttctc agtattatac tccaaccgtt ttacttattg
     1861 cgttggttgt tggtctgatt agtcgtgatt ttcgtttagc aatcactgtt ttggttcttg
     1921 gttgtcctgg cgctttagtc attggtgcgc ctgtttcgaa tgtagcagga atcggtaatg
     1981 gagcgaaacg gggcatctta gtcaaaggcg gtgaagcaat ggatactttt tccaaaatcg
     2041 atacttttgt ctttgataaa actggtacat tgaccaaagg aactactgct gtatccacta
     2101 tcaagaacta tacagaggat gaaggggaca gcttagcttt agtggctcga ttagaaacgt
     2161 tatccgatca tcctctgggg cgcgccgtga tccattatgc tcaggagaaa gacattgatt
     2221 ttcaacaatt agcagtcaaa gataatcaga caatcaaggg acaaggaatc acagctgaga
     2281 ttgatggtca tacagtttgt gcgggcaaca ccaaattgat tgccgctcac aaactttctc
     2341 tcacaccaaa gcaagtacaa gatttacatc aattacaaca aacaggcagc tcggtcatta
     2401 ttgtagcaat cgacaaaaaa atcactcaaa tcataggtgt gtcagatgtg attcgaccag
     2461 aagtcgctga acaattagct aagttgaaac aagcaggagc aaaacagtta gtgatgctga
     2521 caggtgataa tcagatgact gctgattatg tagctgatat gttaggcatt gatgaagtcc
     2581 atgctgaatt attaccagac gaaaaggttc aatttgtgaa aaaataccaa gaacaaggat
     2641 tgcgcgttgc ttttattgga gatggaatca atgattctcc ctctttggct gcggctgata
     2701 tcgggattgc tatgggatca ggaacggatg ttgctatcga aacgagtgac gttgtcttga
     2761 tgcaatctaa ttttgaatcg ttggttcatg cctaccgctt ggctaagaaa actgttttaa
     2821 atacccgtga aaatgtagtg attgccattg gtgtcgtctt atttttactc aacggcttat
     2881 ttgccgggtt tatttacatg gctagtggga tgttcgttca cgaagctagt atcttgatcg
     2941 ttatctttaa cgcgatgcgt ttattgtatt ttggacacga aagcaaagaa gaaaaatcgt
     3001 aaaacttgat aagaatcaat ttttttatgg aagctgtaat ctagaataag actatcaaga
     3061 aaaaggaagg aagaactacg atgaaaaaaa ttattatgca attagataca ctagcttgtc
     3121 caagctgtat gcaaaaaatc gaacatgcgg tttctcaaca gcctggggta agcaatacga
     3181 aagttttgtt taatgcgagc aaaatcaaag caaactttga tccagatgaa gtccaccctg
     3241 aacaattagc tgcagttgtg gaaaaattag gttatgaagt ccaatcagca aaagtaaaag
     3301 aggcgtaaat caatgaatat agtggaagaa aaatttgttc aagaaacaaa acaagcagat
     3361 atcgaccatc ataaaccaac tgctggcgcc atggtggggc atgtattagc aaattttaaa
     3421 ttagaagaaa tgaaattgat ccaagcacaa ttgtatgttt tgggtacatc ttcgcaagaa
     3481 ttaagtaccc ttttttcaga atatgctgat caagaagcga gatggttcaa taaaatctct
     3541 catgagttag tgattgaaaa tgaaatcgtt ccgtctactt tagaagaagt catgcgttat
     3601 ggaaaaattt acgaacatgg tcaaaacaaa tatttaccag cctcaacaat gttggaaaac
     3661 tttgcaaaag attttgattt ccaaaatgtc tttatcacac gggcaatcaa attagctgaa
     3721 aaagaagaaa aatatggctt gcaacaattg cttgttgaac ttttaagttt caataaagaa
     3781 atcatctacc aagtacaaag actccaagga aaaactgtcc gacaagattt ggatgaggaa
     3841 gacgatgatg actaaaagtc tatgtttctt caaatacaaa tataaagcgt gaggtaaatt
     3901 ggaaaatcac gaagattaaa ataaaagaag cacccgctga tgtattacag acaacatcag
     3961 cgggtgcttc tcagttttta gaagatttga aagattgatg agaaaatacg aaaaatcaat
     4021 aaaatcacat tgcttatttg atagaataaa gaagtgggag taatcgaaag gagtttttta
     4081 attgaaaaaa aggattttag taacaattgg tctagctttt ttgttagctg gttgtaccaa
     4141 cgggaatgaa tcgaaaaaag aatcaacaaa agaaacagcg aaagcaacag ttgcaactac
     4201 tacaacaact agcacagaga gcaagtcaac tgcagcaagt acagaaacag cagatacctc
     4261 tacttcgtca gagtcttcca aaacgactga aacaactcaa acatctactt caagttcttc
     4321 tgtagaggct tcatcttcct cttcagaaag cagcgaacaa gttgaacaaa cactgtggaa
     4381 tgcagacaaa tccgcacaat tagaggcatt tatgaccaaa tggggccaaa caatgggaca
     4441 acaatacaaa agttatacga atcaaatgag tgtggactta tatggtctta aagtccctca
     4501 agctattttg aacggggagt ggaagatggc tattggtgga gtgcctgttt ctgcagaatg
     4561 gtcagaaagt gggacaggtc aagcagacta tcaaatcaca gccgtctatt ccgatgcaga
     4621 gacagaacca tatctgaaaa agcacgtcta tctattcggg tttcagcaaa atcagccaaa
     4681 agtattggtg acacaacaaa atcaggggaa tcccgataat tatctttact tcaacgagac
     4741 agcgaataat gaattgaaaa atggctttaa tcaaatcgtt tatggacaag caaattaatg
     4801 agataaacaa ggggttgtga catgtctttt atcacaaccc cttgctcagt ttatttgtta
     4861 gtcttttatt ttttgtgtcg cccatgttgc actgtactgg ttacctgcaa caagatcata
     4921 ttgtatcaga cgataatctt ctagaatttc tttttgtttc tcagttaaat ttttttcttg
     4981 gataatttga ctatcctctg taacaaagac ctgttttcca ttaaaacgat tagacaagct
     5041 ttctgtagga tcaattgtta atgcaggtag atgattagat acttctgtca acaacgcata
     5101 gtaaggagtt actttgatat tcgcatgctc aagagccata gcaccaaagt tattaggagc
     5161 aacaacttct ttatttaacg tagctgcttg ttcacgacta tacttgttgt tataaatgaa
     5221 ataatctgtc tggtgctgtt ctaatccata ttttttcgaa tcattcccac tatagattgc
     5281 gggtaaatgg tcaccataaa aaacaaaggt aatcggttta tcgattttgt cgatttcttg
     5341 gatgaatttt tttactgctg tatcggtata gtgaaggcct tgcatataag tttgtaattc
     5401 ttttcttcta ttaggaatga cagcagttcc ttcagcagtg tagttcaatt cactatagta
     5461 atcatcataa ggcatatggt tttgcatagt ggataattgg atgaattgtg acgtttccgt
     5521 attgctttgt aaagctttca aggtttcttt gtatgctgaa tcatccgata tgcgtgggct
     5581 ttgttctatt ttatccgtat aagttaattt atccggactg tctaaataat agaacctgtc
     5641 aaaaccaaat ttatcaaaca cttgttttct gcgataaaga gcagctgtaa aaggatggat
     5701 ggctattttt tcatcgaata gatcggtaat atttggactt attttctgac gctccactag
     5761 ctgggtataa ggtgtaacta acgaagcaga caggttactg atactcaagc ctgtcagtcc
     5821 ttcccattcc atgttagctg ttccgccacc ataaccgacg gataacatca atcctcctgt
     5881 tccttctgtt ataatgcttt tgatgtaagg aatagggtct gtaggtactg tcagattagg
     5941 gacacgtgca ggatcactga agctttcact taaattgaga acgatcgtct gattgtttaa
     6001 ccagtcactt cttgtttggt tgatttcatc tgcctttgtg ttgtactttt tcattattcc
     6061 attaattttt tctttcgaat agccagcagg ttcttccatg attttaatgt cgatattgtt
     6121 taggaattga atgactggtc cgttatcttt tacggcgcct tgctggttaa agaaagtctt
     6181 gtttacttta aataaattaa acagcaagaa agaaggcgaa tttttgtgat taatgaaaaa
     6241 gacacccgaa aaacatccta ataatacagc gatactgata aatcgacgtt tccaatgaat
     6301 cttcaaggaa tactgttttt gtagtctacg ttgtacaata atactagtaa tcaataatag
     6361 gagaaggatc aatccgccca cgattaataa gactgggcta accatcgata atagttctga
     6421 caatccggtc agcattttta aatctgaagg aaagacaggc tcttcccgca tttccatttt
     6481 gattactgtc gaaatagtga gcagtaagtt aatgatcaag gtagcagtca aggcgtacca
     6541 aaaacgattg gaaatcaaca atagtaagaa gaaaaatccc ataataatga tgattgtcag
     6601 gatcatgggt gcttgtcgct gagtaaatat ttttaaggca acctggatag tttctttcca
     6661 accttctttt ggttctaaaa cgaaaatctg cacgaaggta aagcagtaaa aaaatgctgc
     6721 aactaaaaaa taacttttat gaacaaaaag ccaatgaatt ctttttttcc aacgctgagt
     6781 taattgttca atcgaatcaa aagcgaagta ttcgatgaaa cgatgaatga aacggatttt
     6841 ttgtatataa aataaaaaga taactaggag ggacactgct aataaatatc aaataaaaaa
     6901 ctgcccgata tttatcagcc atgccttccc tgagtttgga aattcacgtt tcataaatgt
     6961 agatacgcga taagaaacta aagggaaatt acatataact tgagtgacaa tcaaaaatac
     7021 agcaagaaaa tttcctcttc cttttatacc taatttatca gatagcattt ctaaaaataa
     7081 aaacgaagag aaagtataat acattgcgaa gagagaatat ggtttagaaa acctcagcgc
     7141 tgtatctgta tttcctctga tgatgagtga aatttgtgtc atcaaaataa tcaaaccgaa
     7201 taaaacaatc aagctgataa atacttgagg aagcttatag cggacctttt ctttccagtt
     7261 ccattctttc aaaccataac ccattaaaaa taaataaaaa atccataaaa tactctgtcc
     7321 attctgtaat gcaaacgggt ctttattaaa tagagtagag cagccaacta atccaattgt
     7381 taaaaataat agtagatatt taattgttgt tttcgataat ttatttaaaa atccaactgt
     7441 ataaggaata ctacaaaaaa gcaaagtaca agaaacagca taagtaaagt aattctgact
     7501 gataggtgtt aggataagcc aataatcacg aatattcaag ctgccagctt gtacgtaaaa
     7561 aaagatattg caaactagtg tggctattag gaatataaag gcaaaacgac ttaaataatg
     7621 taaaactttt aaacgggcga ttttttgata ttggtaaccc atgtaaagtg ggatcaaatt
     7681 tgcaccaata acaacggaag tttgtaaaaa tgtatagatg tagaatcgac cagggtttgt
     7741 tgcatatgta ggaatagtga ctgctgaaat caatatacgc tgtaaaacca tcattataca
     7801 agcgacaaag attgtcagga agccataact ctccttcttt atttttttca agaataaaca
     7861 cctcacaaaa aaattggaca aaaacataag aaaacgtttc ctgataagtt gcccattaat
     7921 aacatcctaa cttagaatat ctcaaaaaac aacgtgtaga cagaaaaagt acaaaattag
     7981 caagaaatgg ttgtttgtat tgttagaatt tttagaaaat tataccttta gtttctttgc
     8041 ataaaatgat acaatgattg ttaaaattgt gaaagggtgg tcatttgtta gagaagtttt
     8101 tggatatgtc gttagttcaa cgtattgttg ttggtatttt agtcggaata ttgtttggta
     8161 tctttttccc cacatggaca tttatttcaa tattgggaac gttgtttgtc agttgcttga
     8221 aggcaatcgc acctattctg gtttttttct tgacgatggc gtcaattgcc aagcataaaa
     8281 ttgggaataa gacattcgtg aagcctattt tgatcttata tttagtggga acattgcttt
     8341 ctgcacttgt agcagttatt gtaagttcaa tttttactgt tcctattact ttacaagaaa
     8401 cagtatctga aaaagcaccg caaagtttgg aaagtgtttt atctactatg ttgacgaatg
     8461 tcacacaaaa tcctgttcaa tctcttattg atgcgaatta ccttggcgtc ttattctggg
     8521 ctgtattgtt gggactagct tttcgagcac gttcagaatc gacaaaagag ttggtggatc
     8581 agatatctat agcgttgtca caagtcgtac aagtgattat tgcatttgct ccgataggga
     8641 ttttaggatt ggtttatcaa tcaattgcta cgactggaat cgctggattg gcagagtatc
     8701 tgcagctttt acttgttttg atcggtacga tgctgtttgt cgcattagtt gtgtacccat
     8761 tcctcacgtt tttgtttatt aaagaaaatc cctatccatt gattttcttt tgtttgaaag
     8821 aaagcgcact tcccgctttc tttacccgaa gttctgccgc aaatatcccg ataaacatga
     8881 tgttggctga aagattgaaa ttgacaaaag agtcttattc catatcgatc ccgttaggtg
     8941 ctacgatcaa tatgggcggt gccgcgatta ccatttcctt gatgacgttg actgctgtac
     9001 attctttagg gattgaagca ccttttgttt tgaaagtcct tttatgtatt ttgtctgctt
     9061 tagctgcttg tggagcttca ggtgttgcgg gcggttcact gttattgatt ccattagcat
     9121 gtagtttgtt tggtatctct aatgatgtcg cgatgcaaat cgtcggtatt ggatttatta
     9181 tcggtgttat ccaggattca atggaaactg ccttaaattc gtcaagtgat ctgttgttta
     9241 ctgcaatagg agagctaggc gcgcgtaaaa gaagtgggga aaaaatcaaa ttgaaagatt
     9301 gtttagatgg attaagtgaa cgttgaacgt tagatattta gtatttttaa aatgattcga
     9361 gtttttaatg agttatatag atgttcgtgt aaatagaggt ttctgttaaa tcaagataaa
     9421 gtcgtttata ataacaatta aaagcatagg taattgttat tggaggcgta tcatggattt
     9481 tttacgtaaa tttggttttt tctttttatt attaggcgtg ctgagtgatt ttttgactcc
     9541 gtacgtatta ggattgtatt atcctgaatt agatcaaatg aagagcgtga tcagtgtatt
     9601 tggagaggtt tccagtcctg taagaaaagc gtttcttatt tggtctgtgg tttcagggat
     9661 ctttttcatc ctgtcattgc ctgcattgta tgctgcattt gttagtacat ctcgtgtgtt
     9721 agctacccta gttacagcag ccatcgcgtt ctatggtata ggtgattgta tctttacagg
     9781 tttgttcagt atcaataccc atgaatcttc gtggaatgtc tcgacatggg tccataatat
     9841 cggatcaggt ttaggttaca gtggtttttt attgtttcca gttcttcttt tcttgatcta
     9901 tcgaaaaaat gggcaaatgg acgtaagcca cttatacctt ggactgtctg tgatcagttt
     9961 gctttttgcg gcgatttatg gactggcaag aattccttct ataaattctt ggccattact
    10021 gaataaaata ggtttttgcc aaagaatcag ttttttcttt aattatgtgc ccattgcctg
    10081 gttgagtatc ttgcatctca aacagtaaaa cttgagccgt caaattgaac agtcagcgga
    10141 tacttcatcc ggtggctgtt ttttgttttt ctgaaatata tatttccaca gttttcttgt
    10201 atagcttctg tcttagagaa gcaaggagct atattttgtg ctacaatctt aaaaaggtag
    10261 agagaagacc aaatggaaga cagaggagcc actaaaatat gagaaacatc aagttgacaa
    10321 tcgaatatga cggaaaaaga tattcaggat ggcaaagact tggagatgac gataaaacca
    10381 ttcaaggaaa aatcgaaaaa gtattgcatc agatgacaaa tgaagagata gaaattattg
    10441 gatcaggaag aacagatgct ggcacccatg cgcgaggaca agtagcaaat tttaaaacaa
    10501 acacagaaat tggactggca gaaatgatcg actttatgaa tcgttacctt ccgcgggata
    10561 ttgtcatcaa aagaatcgaa gaaatgcctg agcgcttcca ttcaagatat aatgcggtgg
    10621 gcaaaaaata cagctattat gtttggaaca acgtgattcc ttcagcattt gagcgaaacc
    10681 acagctttta tttccctcaa gagttggata tggacaagct gaatgcagct tgcgaaaaat
    10741 tagtgggtac acatgatttc attggttttt ctgcattgaa aaaatcaaaa aaatccacag
    10801 tgcgtacgat tgatgaatta tctatcgaac gaaagggaga aatgcttcat tttacttttg
    10861 tcggagacgg attcttatat aaaatggtgc ggattatcat ggggacatta ctagaaatcg
    10921 gcacaggtgc catgccgata gaaacaatcg atgaaatatt tgaaagcaaa gtgcggcaac
    10981 atgcaggaga aaccgtccct gcacaaggtc tgtttttgga tgaagtttat tatcgataaa
    11041 aatactttga taatggatga aacgcatgtt tcacagtata aatatcgatg ttaaataatg
    11101 tatgaagcaa attaaagaaa tataaaaaaa gactttttag cctgtttatg tgtacagcta
    11161 aaaagtcttt cttaaataaa aatctagtga gaatgttgta cttctcttct cggttgtttt
    11221 tgtttagaaa aagtaaatcg ataaagtaaa aataaaatca aagtaacgat agataaatat
    11281 cttgaagcaa ttgctagttt tgtgtactga taagtaataa cgactttatt tatacctgga
    11341 ggaatagtta attcagttgt gccaaactta gataattttg tttgtactag tttgccatta
    11401 atagaagcca cttgcccttt ataacgataa ataggtgtcg tcaagacagt atttttatta
    11461 ttcgaattat ctaattcaat tgtaagtttg ttactcataa aatgagttgt tggatttatt
    11521 tcctggttac ctagtaaata ttgatcatta ttaatcatat caggatgata gattgactct
    11581 ttgtttgcat aatcagtatg attgtaattt tcagctatag caacagcgtc ttcaggtgct
    11641 ataagtgtta atgaatcttt gttagcatgg atagaattgc ttactcctga gagatgcaat
    11701 attagtgcaa gaaacgtaaa gaccattatt ttccatgaat tatttttagt agaaaaaata
    11761 taatagatca ctatactcat cgtataggct ataaataaag aagaaaaacc atttaaccgc
    11821 cagacaaatt gtagactctt aactggtgta tggttaaaaa gttgccaagg aaataaattg
    11881 gttgaacaaa aaagaacaaa tacaccaaga aagaaaataa ataaatcatc agcagttaat
    11941 ttttttataa aaataaaagc accaatcaat ccgagaaata agaataatcc aatagtatag
    12001 ttgttatagt tattatttaa aatatgtgtc agcatatcac taggagccat accacttaat
    12061 tcttttccca gtgggacttt caattcttgt gctctagatt gttgaataaa aggaattaag
    12121 aatcccgcac ttaaaaaaaa ggtgaggacg gtagctttta aaagacttaa gagtctagcg
    12181 attttttggt cccaaaaata aaaagaaaga aacaacgttc ctccaataaa tacagaaacc
    12241 atagcgacag aaagtaaatg agtatacact actagcgtca tgccaataga caaccagtac
    12301 catttttggt aatctctaat ataaatttcg taacacccca ttagtatcaa aggcaaaaat
    12361 gttaaagcga cagcttctcc caatgaagca cgatggaata tgtcaatggc acgataggcg
    12421 gaaaatgtat aaataatcga aaataacagc gaaatataac gattgttttt tatttgtttc
    12481 atagaaaaat aagaaacgac cattgttaaa aaagtgataa aaaaatagta gatattataa
    12541 cttaaaacaa gatttcccat cattttataa aataaaaaag ctggataaag agtaagccat
    12601 ggataaaaaa tattcatcat tgttccatga tgatcgaagt tattgaagtt tactggagaa
    12661 ctccatacat tatctagtcc tatgattcga gataaatgaa agtaagtatc ctgattgaca
    12721 ctatgataga ttcccttaga catgaataga gggatgacgt aagcaaaact tagaaataac
    12781 ataaaaatga tatctattat ttggttttt
//
LOCUS       DAZKLM010000081        12746 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3764
            SAMEA115706913-rid22582563.denovo.084, whole genome shotgun
            sequence.
ACCESSION   DAZKLM010000081 DAZKLM010000000
VERSION     DAZKLM010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706913
            Sequence Read Archive: ERR13196647
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12746)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12746)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287823.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287823.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,004
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,849
            CDSs (with protein)               :: 2,849
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 41 of 96
            Pseudo Genes (incomplete)         :: 52 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 15 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12746
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706913-rid22582563.denovo.084"
                     /isolate="VRE3764"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(85..264)
                     /gene="rpmF"
                     /locus_tag="ACG842_002131"
     CDS             complement(85..264)
                     /gene="rpmF"
                     /locus_tag="ACG842_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HFQ0248649.1"
                     /translation="MAVPARRTSKAKKAKRRTHYKLTIKGLNECPNCGEMKKSHHVCA
                     NCGYYDGKDVMSKEA"
     gene            complement(322..891)
                     /locus_tag="ACG842_002132"
     CDS             complement(322..891)
                     /locus_tag="ACG842_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HFQ0248650.1"
                     /translation="MKWSLLELRKYQETPLTFHETLDLKAALMKRDNLILDVAPVEVE
                     GLVSVDKKGYIVHYTVQTTLTVPSTRSLEPVELPMDFSVDEVFMTEEQFQSRDEQMSA
                     DEILLIEGPLLNLAESIEDNILLAIPMRVLTKEEEAATELPKGNDWEVVSEEEYQRRK
                     MEEAENKIDPRLAKLSELFDSKSKDDDNK"
     gene            1077..1979
                     /locus_tag="ACG842_002133"
     CDS             1077..1979
                     /locus_tag="ACG842_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFQ0248651.1"
                     /translation="MNTKDIEYYVQIVKQKNFTKVANYFRVSQPTITYALQRLEKEFD
                     TVLITRDRSHHELIVTPSGKQLFLHAQTILQEISTARAEITQLQKKKLRFGMPPIIGN
                     EYFPKLSSYFIKHELMNQIEITDGGSRDLYGMIRQGRIDLAVLGSTQPIVDQYLETEL
                     LLDKRFMIVVPPTHPLSKQDTVSFKDIADEQFVLLNEHYVHPAAFTKLSKQTHTHPKV
                     VYQSNDLTILKSMVRENVGIGFLAEIAVHPEDHLVCLPLSDEVQPRFLISLAKRTQQI
                     VTPLQEQVVQVIHEFVTAQKNSSS"
     gene            complement(2014..3798)
                     /gene="pyk"
                     /locus_tag="ACG842_002134"
     CDS             complement(2014..3798)
                     /gene="pyk"
                     /locus_tag="ACG842_002134"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0030955 - potassium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HFQ0248652.1"
                     /translation="MKKTKIVSTLGPASNSVEIISQLIESGANVFRFNFSHGDHEEQL
                     SRMTMVREAVKKTGKDVGILLDTKGAEIRTTVQGTTEADFGRAGYIKFEVGDQTRISM
                     DPEHVGSKEKIAVTYPGLFDDVHVGGHILFDDGLIDMQIIEKDEANRELVVEVKNAGM
                     LGSRKGVNAPGVSISLPGITPKDADDIRFGLDNDIDFIAASFVRKAQDVLDIREILEE
                     KDMTHVQIFPKIESQEGIDNIDEIIKVSDGVMIARGDMGVEIPAELVPMVQKRIIKKC
                     NAAGIPVITATQMLESMQENPRPTRAEASDVANAVFDGTDATMLSGESANGDYPVEAV
                     STMARIDQEAENALSELGTFQLNEFDKTDVTETIGLSVARAAKNLGVKTIVAATESGY
                     TAKMISKYRPDADILAVTFDERTKRGLMLNWGVYPTVAEKPSTTDEMFELAAKKAVEL
                     GFASEGDLILITAGVPVGERGTTNVMKIQMIGSKLLEAQGVGQKSVVANAVIASSAEE
                     AVKKAKDGMVLVVPSTDKEYMPAIEKAAAVIVEEGGLTSHAAVVGIAQDIPVIVGAKG
                     ATTTITEGELITVDSRRGIVYRGETTAI"
     gene            complement(3872..4834)
                     /gene="pfkA"
                     /locus_tag="ACG842_002135"
     CDS             complement(3872..4834)
                     /gene="pfkA"
                     /locus_tag="ACG842_002135"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010746303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HFQ0248653.1"
                     /translation="MKRIGILTSGGDAPGMNAAVRAVVRKGIYEGLEVYGINYGFAGL
                     VAGDIRRLDVADVGDKIQRGGTFLYSARYPEFATEEGQLKGIEQLKKFGIEGLVVIGG
                     DGSYHGAMALTKRGFPAVGIPGTIDNDIPGTDFTIGFDTAINTVLESIDRIRDTATSH
                     VRTFVIEVMGRNAGDIALWSGVAGGADEIIIPEHDFDMASVAKKIQEGRDRGKKHCLI
                     ILAEGVMGGNEFAEKLSEFGDYHTRVSILGHVVRGGAPSARDRVLASKFGAYAVELLQ
                     EGKGGLCVGIHDNEIVASDIIDTLENNKHKPDLSLYDLNNSLSF"
     gene            complement(5002..8322)
                     /gene="dnaE"
                     /locus_tag="ACG842_002136"
     CDS             complement(5002..8322)
                     /gene="dnaE"
                     /locus_tag="ACG842_002136"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HFQ0248654.1"
                     /translation="MFFPQLYTITSYSLLQSTIRIREYVQEAKKQGYTSLAITDKDVL
                     YGAPEFYRICVEEGVQPIIGLHLSYTYEEKNYGLLAYAKDEIGYQQLMKLSSRKMIQE
                     KLELAEMTETDHLAFVVPANHSVSEAFNKEDNFFGHWQFLLENVFTRNFYIGLTENTP
                     DQLPEWYQYVIKENYSLCALHPIASLQQEELFALEVMKHIKEGSQIPLENLSASLPLT
                     ANVYLLSEQEMIKRFEKAGLSQAVIGAQRLAESISFEFQFHQKLLPHYPVPNGKQAGE
                     YLRELCYSQVERRITDYTKEYQERLDYELSVIHQMGFDDYFLIVWDVMAFAHRNKIVT
                     GAGRGSAAGSLVAYVLEITDVDPLEYDLLFERFLNPERFTMPDIDLDIPDNRREEVLN
                     YVREKYGQYHMAQIATFGTMAAKMVLRDAARVFGLSQSEANRWSKAIPNQLKITLTQA
                     YKTSHQLVELVQASEKNQLLFKTAVILEGLPRHVSTHAAGVVISDKNLLELVPLQRGS
                     DEAFLTQYTMNEVEAIGLLKMDFLGLRNLSIIDFTLKAVKRVEGYEIRLKEIPLNDAK
                     TLLLFQRGETSGVFQFESAGIRNVLRRLDPENIEDIAAVNALYRPGPMQNIDTFIARK
                     KGKEAIRYPDDSLIPILKNTYGVIVYQEQIMQIASTMAGFTLGQSDILRRAVSKKKKD
                     VLDEEKSHFVSGALQQGYSEKTAATVYEYIEKFANYGFNRSHAFAYSFVGFQMAYLKA
                     HHPGAFFTSLMNSVRHNTSKLKEYIAEARKNKLKLSSPSINQSNSSFELVNKEEIRFG
                     LTAIKGIRRDFVEDILNERKQNGPFQSVDQFLIRIDKRWLKLELLQSLTAVGVFDELS
                     PNRKQLMLDLEGKIQNIVYSGGSLDLLGIMALKEEEVADYTLEERLKLEEEYLGIYLS
                     GHPAEGFDRLRLAKQVQLITDIVPNQPVNLLVMIKEVREIRTKKGELMAFVEGTDSSG
                     EISVTVFPTLYRKYRNLFAVDRVIFVQGRSEISKYNQELQLIGESFADPNQLEAQYPS
                     QTCYLRITEEREDPEIMKQLQVIFQKHPGYIPVVMYHEKTQRKVVLSDAYWVDDSKSK
                     SIKGQLIYLLGEDNVVFK"
     gene            8553..8738
                     /locus_tag="ACG842_002137"
     CDS             8553..8738
                     /locus_tag="ACG842_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzD family protein"
                     /protein_id="HFQ0248655.1"
                     /translation="MRYIVTLFWAVVLGQVVGYIGAALTSGTYDFTLTTIISFIAGVI
                     ILLIGAVAPRKETSAHS"
     gene            complement(8800..9996)
                     /locus_tag="ACG842_002138"
     CDS             complement(8800..9996)
                     /locus_tag="ACG842_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HFQ0248656.1"
                     /translation="MEINWRKNLVIAWIGCFFTGASISLVMPFIPVYVEQLGTPKDQI
                     ELFSGLAISVTAFASAVVAPIWGNLADRKGRKLMMIRAAAGMTLTMGSLAFVPNAYWL
                     LIMRFWNGILSGYIPNATAMIASQAPREKSGWALGTLSTGAIAGNLIGPSMGGALAQW
                     FGMENVFLITGALLMITTVLTIFLVKEDFHPIEKKDLISTKEIFSKMDHLSILIGLFI
                     TTLILQIGITTISPILTLYIRELSGSTENILFVSGLIVSIAGVSAVFSSPKLGKLGDK
                     IGNQKVLIGGLILSFCCYLPMAFVTTPLQLGILRFILGFSTGALMPSVNTLISKITPQ
                     EGVSRIYSYNQMFSNFGQVLGPMLGSTVAHAYNYSAVFIVTSLFVLSNILLSLFNFRK
                     VLRSRL"
     gene            complement(10166..11602)
                     /locus_tag="ACG842_002139"
     CDS             complement(10166..11602)
                     /locus_tag="ACG842_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HFQ0248657.1"
                     /translation="MEKFFGLKKNNTNVSTEIMAGVTTFFAMSYILFVNPTILSASGM
                     PFQAVFLATIIASIIGTLVMGLFANVPYAQAPGMGLNAFFTFTVVFGLGYSWQQALAM
                     VFICGLINIFITVTNIRKMIIRAIPESLQHAIGGGIGIFVAYVGIKNAGFLSFSADQS
                     AISSSVVEGGKATNVTINGGIVPALANFDNAPILLAVIGLVLTAILVVKNVRGAILIG
                     IVATTVLGIFMGVVDLSSIDWQTNSLGNSFKELGTTFGAAFGSEGMQSLFSDSSKIPQ
                     VLMTIIAFSLSDTFDTIGTFIGTGRRTGIFSKEDEIALEDGRGFKTKMDKALFADAIA
                     TSVGAIFGTSNTTTYVESAAGIGAGGRTGLTSVVVAVLFALSSLFSPLIAIVPNQATA
                     PALILVGVMMMASFADIKWLDMEEALPAFFASIFMGLCYSISYGIAAGFIFYTIVKVI
                     KGKYNQVSVALWIVDILFIINFIILATI"
     gene            11895..12704
                     /locus_tag="ACG842_002140"
     CDS             11895..12704
                     /locus_tag="ACG842_002140"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFQ0248658.1"
                     /translation="MNKKLIAIDLDGTTLNQDSMITSKTKETLKKAINAGHHVSIATG
                     RPFRMSHQFYQQLELTTPMVNFNGALVHIPNQHWDGERETLINREIAFEILSQKKQLN
                     LDFIAAENRDTFFIDSFDFFDEKIFASSRPGEKNLLSPKNLTTNPTSLLVRTDKRFAG
                     AVSAELTRQFGSYVDVRTWGGPTAILEIVSKGIQKAKGVQEIANYLSIDQKDVIAFGD
                     EHNDLELLDYAGWGVAMANGTDQLKGIANDVTPLSNQEDGLAVYLEKLLKL"
BASE COUNT         3804 a         2675 c         2250 g         4017 t
ORIGIN      
        1 attttttatt taccgactac aaaaaaataa acctcaaggc tccatggtta gccttgaggt
       61 tttacatttt ctaattaaaa tgtattaagc ttcttttgac attacgtcct taccatcata
      121 gtaaccacaa tttgcacata cgtgatggct ttttttcatt tcaccacagt ttggacattc
      181 gtttaaccct ttgattgtca atttgtagtg tgtacgacgt tttgcttttt tagcttttga
      241 tgttcttcta gctggtactg ccattttgct acacctcctt gtaaagtctt gtatctaaat
      301 tagacacaat atattccaat cttacttgtt atcgtcgtct tttgatttag aatcaaaaag
      361 ttcagatagt tttgcaagac gaggatcaat tttattctcg gcttcttcca tttttctgcg
      421 ctggtattct tcttcggaaa caacttccca atcattgcct tttggaagtt ctgttgctgc
      481 ttcctcttct tttgtcaata cccgcattgg aatcgccaat aagatgttgt cttcaatcga
      541 ctctgctaaa ttcaataatg gaccttcaat tagcaaaatc tcatctgcag acatttgttc
      601 atctctactt tggaattgtt cttctgtcat gaatacttcg tctacagaaa aatccatggg
      661 cagttcaaca ggttctagtg aacgtgttga aggaactgtc aaagtcgtct gcacagtgta
      721 atgaacgata tagcctttct tatcaactga gaccaaacct tcgacttcca ctggcgcaac
      781 atcaagtatt aaattgtctc gcttcatgag agcggctttc aagtcgagcg tctcatgaaa
      841 agttaacggt gtctcctggt attttctcaa ttccaataaa gaccatttca tttttcatca
      901 ctcctaagca acaaaagcaa ttatacaggg caaaaagcat gttgtcaatg aaattttctt
      961 gacaccttgc agttcataaa aaaacagaaa aatctttatt agaaagcctc tgtttgctat
     1021 catcctcaga atcatttaca ataatcatta ttccattaaa ggagcggatg atcttcatga
     1081 acacgaaaga tatcgaatac tatgtccaaa tcgtcaaaca aaaaaatttt acgaaagtgg
     1141 ctaattattt ccgtgtgagc caacctacta ttacatatgc attacaacgt cttgaaaaag
     1201 agtttgacac cgtacttatt acaagagatc gatcgcatca cgaattaatc gtcacgcctt
     1261 ctggaaagca gctgtttctc catgcacaaa ccattctcca agagatttct actgcaagag
     1321 ctgaaatcac tcagttgcaa aagaaaaagc ttcgttttgg catgcctcct attatcggca
     1381 atgaatactt tcctaaactg tcaagttact ttatcaagca tgaattgatg aatcaaattg
     1441 agattaccga tggaggctct cgggacttat acgggatgat ccgacaaggg aggatcgatt
     1501 tagctgtatt aggctcaaca cagccaatcg tggatcaata tttagaaaca gagttacttt
     1561 tggacaaacg tttcatgatc gtcgtgccac ctacacatcc tctttccaag caggatactg
     1621 tctctttcaa agatatagca gatgaacagt ttgtactatt gaacgaacat tatgtccacc
     1681 ccgcagcatt cactaagctt tctaaacaaa cacatactca tccaaaagtt gtttatcaaa
     1741 gcaatgactt gacgatttta aagagtatgg tccgagaaaa tgtgggtatc ggttttttgg
     1801 cagagattgc tgtccatcct gaagatcatt tagtttgcct ccctctttca gatgaagttc
     1861 agcctcgctt cttgatttca ttggcaaaac gaacacagca gatcgtcacg cctcttcaag
     1921 aacaagtagt ccaagttatc catgaattcg ttacagcaca aaaaaacagc tcttcgtaaa
     1981 agaagagctg tttttatttt ttattaagct aaattagata gctgttgttt cgccacggta
     2041 aacgatgccg cgacgtgaat caacggtaat caattcacct tctgtaattg ttgttgttgc
     2101 gcctttagca ccaacgatta caggaatatc ttgtgcaatt cctacaactg cagcgtgaga
     2161 agtcaatccg ccttcttcaa cgataacagc agctgctttt tcgattgcag gcatatattc
     2221 tttgtcagtt gatggtacaa ctagaaccat tccgtctttt gcttttttta cagcttcttc
     2281 agcagatgaa gcaataactg catttgcaac gacagatttt tgtccaacgc cttgagcttc
     2341 taataattta gaaccaatca tttgaatttt catcacgtta gttgttccgc gttcaccaac
     2401 tggtacacca gcagtgatca agattaaatc gccttctgat gcgaaaccta attcaacagc
     2461 ttttttagct gctagttcaa acatttcatc agttgttgat ggtttttcag caactgttgg
     2521 gtaaacgccc cagtttaaca tcaaaccacg ttttgtgcgt tcgtcaaaag taacagccaa
     2581 gatatctgca tctggacgat attttgaaat catttttgca gtgtatcctg attcagttgc
     2641 agcaacgatt gttttcacgc ctagattttt tgcagcacga gctacagata aaccgattgt
     2701 ttcagttaca tctgttttat cgaattcgtt caattggaat gtacctaatt ctgataatgc
     2761 attttcagct tcttggtcga tacgtgccat tgttgaaaca gcttcaactg ggtagtcccc
     2821 gtttgcagat tcaccagata gcattgttgc atctgtgcca tcaaatacag cgttagcaac
     2881 gtcagaagct tccgcacgtg ttggacgtgg gttttcttgc attgattcta acatttgtgt
     2941 agcagtgata actgggatac cagcagcatt acattttttg ataatgcgtt tttgaaccat
     3001 tggtactagt tcagctggga tttcaacacc catatctccg cgggcaatca taacaccgtc
     3061 agaaactttg atgatttcgt caatgttgtc gataccttct tgagattcga ttttagggaa
     3121 gatttgaaca tgagtcatgt ctttttcttc taagatttcg cggatatcta gaacgtcttg
     3181 tgctttacgt acaaaacttg cagcgataaa gtcgatatcg ttgtctaaac caaaacgaat
     3241 atcgtcagca tcttttggag taatacctgg caaactaata gaaacgcctg gtgcatttac
     3301 ccctttacgt gatcctagca tacctgcatt tttaacttct acgactaatt cacggtttgc
     3361 ttcgtctttt tcaatgattt gcatatcgat caaaccatca tcaaataaaa tgtgtccgcc
     3421 aacgtgaaca tcgtcaaata atcctgggta agtaaccgcg attttttctt ttgaacctac
     3481 atgttcagga tccatagaga tgcgtgtttg atctcctact tcaaatttga tgtatcctgc
     3541 acgaccaaaa tcagcttctg ttgtgccttg aacagttgta cggatttcag ctccttttgt
     3601 atctaaaaga ataccgacat cttttcctgt ctttttaact gcttcacgga ccatagtcat
     3661 acgtgataat tgttcttcat ggtcaccatg agaaaaatta aagcggaaga catttgcccc
     3721 agattcaatc aattgagaaa taatttctac tgagttactt gctggtccta atgtactaac
     3781 gattttggtt tttttcattt aaaaaactct cccattcaat tttgtttatt taaagctgta
     3841 cacagcttaa atagcttggt ttctttcact cttaaaatga aagtgaatta tttagatcat
     3901 atagtgataa gtctggttta tgtttgttgt tttcaagtgt atcaatgatg tcagacgcaa
     3961 cgatttcgtt gtcatggata ccgacacata atccaccttt tccttcttga agcaattcga
     4021 cagcgtaagc accgaattta cttgccaaca cgcggtcgcg tgcgcttggt gctccaccac
     4081 ggacaacgtg tcccaagata gatacacgtg tatgatagtc accaaattct gaaagtttct
     4141 cagcgaattc atttccgccc attacacctt cagccaagat gatcaagcaa tgtttcttcc
     4201 cgcgatcgcg gccttcttgg atttttttag caactgaagc catatcaaaa tcatgttctg
     4261 ggatgatgat ttcatccgct ccgccagcca cacctgacca taaagcgatg tctcctgcat
     4321 tgcgtcccat tacttcgata acgaatgtac gaacatgaga agtcgctgtg tcacgaatac
     4381 ggtcaattga ttctagtact gtattgattg ctgtatcaaa accgattgtg aaatcagtcc
     4441 caggaatatc attatcgatt gttccaggaa tacctactgc tgggaaaccg cgttttgtca
     4501 aagccattgc gccatgataa gagccgtctc ctccgataac cactagacct tcgattccga
     4561 attttttcaa ttgttcgatt ccttttaatt gaccttcttc agttgcaaat tctggataac
     4621 gtgcagaata caagaatgta ccaccacgtt ggatcttgtc acctacatct gccacatcaa
     4681 gacgacgaat atctccagca actaaaccgg caaaaccata attgatcccg tatacttcca
     4741 gtccttcgta aataccttta cgaacaacgg cacgaacagc tgcattcatt ccaggtgcgt
     4801 ctccgccact ggtcaaaatt ccgatgcgtt tcataataaa cattcacctc attaaaatac
     4861 tttatcctaa gtatgactta ggtttacaac catttttgcg ttaaccacga ctaacattct
     4921 aacattattt ttctcacttg tcttgattta cggataaaaa aatgaaaaaa aatgaaagca
     4981 agtagtattt tttaccgtaa gttatttgaa aaccacatta tcttcgccaa gcagataaat
     5041 aagttgcccc tttatactct tactcttgct gtcatccacc caataagcat ctgacagaac
     5101 tacttttcgt tgcgtttttt cgtgatacat aactactggt atatatcctg ggtgtttctg
     5161 aaaaatcact tgtaattgtt tcattatctc gggatcttcc cgttcttctg taatacgcaa
     5221 atagcaagtt tgtgaaggat actgggcttc taactggtta ggatcagcaa agctttctcc
     5281 aattagttgc agttcctgat tatatttgct gatttcgctc cgcccttgaa caaagatcac
     5341 tcgatcaacg gcaaacaaat tacgatattt acgataaaga gttggaaaaa ctgtgacaga
     5401 aatttctcca ctactgtcgg ttccttcaac aaaggccatc agttctcctt tttttgtccg
     5461 aatttctcta acttccttga tcatcactaa taaattgact ggttgattag ggacgatatc
     5521 tgtaatcagc tgaacttgtt tagctaaacg aagacgatca aacccttctg caggatgacc
     5581 tgacaaataa atacctaagt actcctcttc cagcttcaag cgttcttcta gtgtatagtc
     5641 agcgacttct tcttctttta atgccatgat tcccagcaag tcaagacttc caccactgta
     5701 aacgatattt tggatttttc cttcgagatc aagcatcaat tgtttcctgt taggagataa
     5761 ttcatcaaaa actcctactg ctgtcagtga ttgcagtaac tctaatttca gccaacgctt
     5821 atcgatccgt atgaggaatt gatccactga ctggaacggg ccattttgtt ttctttcgtt
     5881 taatatatct tctacaaaat cacgccgaat accttttatc gctgtcagtc cgaatctgat
     5941 ttcttcctta ttgaccaact caaaactgga attactttga ttaatggacg gcgaggaaag
     6001 tttgagttta tttttacgtg cttctgcaat atattctttc aattttgatg tgttatgccg
     6061 aacagaattc atcaggctgg taaagaaagc acctggatga tgggctttta gatacgccat
     6121 ttggaatccc acgaatgaat aagcaaatgc atgtgaccga ttgaatccat agtttgcaaa
     6181 tttttcgata tattcatata cggtcgctgc tgttttttcc gaatatccct gttgtaatgc
     6241 tcctgataca aaatgcgact tttcttcatc taatacgtct tttttctttt tactcactgc
     6301 ccgtcgcaga atatctgact gccctaatgt aaatccagcc atagtcgagg cgatctgcat
     6361 gatttgttcc tgatagacga tcactccata tgtgtttttc aaaatcggga tcagtgaatc
     6421 atctgggtag cgaatggctt cttttccttt cttcctggca atgaaggtat ctatattttg
     6481 catagggcct ggtcgataca atgcattgac tgcggcaata tcttcgatgt tttctggatc
     6541 taatcttcgt aaaacgttcc ggataccagc agattcaaat tgaaatactc ctgaggtttc
     6601 gcctcgttga aacaagagca gcgtttttgc atcatttagc gggatctctt ttaatcggat
     6661 ctcataccct tctactcgct taacagcttt caaagtaaaa tcaataatcg ataagttgcg
     6721 tagtcctaaa aagtccattt tcaaaagacc gattgcttct acctcattca tcgtatactg
     6781 ggtcagaaaa gcttcatcag aaccgcgttg gagcggaact agttcaagta gattcttatc
     6841 gctgataact acacctgctg cgtgagtcga aacatgtctt ggcaatcctt ctaaaatcac
     6901 tgcagttttg aataaaagtt ggtttttttc gcttgcctga actaattcta ctaattgatg
     6961 cgaagtttta taagcttggg tcaatgtgat tttcaattga ttgggtattg cttttgacca
     7021 gcggtttgct tcactttgcg ataaaccaaa cactctagct gcatctctaa gaaccatttt
     7081 tgctgccatt gtgccaaatg ttgcgatttg tgccatatga tattgtccat acttttctcg
     7141 aacataattc aatacttctt cacgtcgatt gtcaggaata tccaaatcga tatctggcat
     7201 cgtaaagcgt tcggggttta aaaagcgttc gaacagcaaa tcatattcta atggatccac
     7261 atctgtgatt tctaacacat aggctactaa tgaacccgca gcagaaccac gacctgcacc
     7321 ggtaacgatt ttgtttctat gagcaaaagc cattacatcc catacgatca agaaataatc
     7381 atcgaatccc atttgatgga tgacagataa ctcgtagtcc aaacgttctt ggtattcctt
     7441 tgtatagtca gtgattcggc gttccacttg cgaatagcat aactctctta aatattctcc
     7501 agcttgtttt ccattaggaa ctggataatg cggaagtaat ttctgatgga actggaattc
     7561 aaatgaaatc gattcagcta aacgctgggc gcctattaca gcttgggaaa gacccgcttt
     7621 ttcgaatcgt ttgatcattt cttgttctga aagaagatag acatttgctg ttagtggtaa
     7681 agatgcagat aaattttcta aaggtatttg gcttccctct ttgatatgtt tcatcacttc
     7741 aagtgcaaaa agttcttctt gttgtaaact agcgatggga tgtaatgcac aaagcgaata
     7801 attttctttt atcacatact gataccattc aggcaattga tcaggcgtgt tttctgtcaa
     7861 gccgatataa aaatttctag taaaaacatt ttcaagtaaa aactgccaat gaccgaaaaa
     7921 gttatcctct ttattgaaag cctcagaaac agaatgattt gccggaacaa caaacgctag
     7981 atggtcggtt tctgtcattt ctgcaagttc caatttttct tggatcattt ttcggctgga
     8041 aagtttcatc agctgctgat aacctatttc atcttttgcg tacgcaagca agccataatt
     8101 tttttcttca taagtataac taagatgtaa tccaatgatt ggctgtactc cttcttctac
     8161 acaaatacga tagaattcag gtgcaccgta taagacatct ttatccgtta tggctaatga
     8221 agtgtagcct tgtttttttg cttcttgtac atattctcga atacgtatcg tactttgtaa
     8281 aagagaataa gaagtaattg tataaagttg tgggaaaaac atcttgcatt ccgccttttt
     8341 tttcgttaaa attcactagc gttaaaaggt ttgatccttt tactttcatt aagatagcag
     8401 gcgctgaagt tgattattcc acttcatttg cttgcttgta atctgtagga atgagttctt
     8461 tcgcaagaac tcaaatttta ttgagatttg gtctcgtttc tttacattca aagaaaatct
     8521 ggtaaaatta agatagaaaa gaggtgttct ttatgcgata tatcgttaca ttattctggg
     8581 ctgtcgttct aggtcaagta gtcggttata ttggtgctgc attaacaagc ggaacatatg
     8641 attttacttt gacaacaatc atttcattca ttgcgggtgt cattatcctt ttgattggtg
     8701 cagttgcgcc aagaaaagaa acctctgccc attcgtaaaa aaacagccaa gagcttgaga
     8761 tttctcaagc tcttttttat tttctactcc tcaccaccat tataatctgc tgcgaagaac
     8821 tttacgaaag ttgaataaag aaagaagaat atttgacaga acaaacaaac ttgtcacaat
     8881 aaaaacagca ctgtaattat aagcatgcgc gactgtcgat cctagcatgg gacctaaaac
     8941 ttgtccaaag ttgctaaaca tctgattgta gctatagatt cggctgactc cttcttgagg
     9001 cgtaatctta ctgatcagtg tattgacaga aggcatcaaa gcgccagttg aaaagcctaa
     9061 aataaagcga agtataccta attggagcgg tgtagtgacg aatgccatag gtaaataaca
     9121 gcaaaatgat aaaatgagtc ctccaattag aactttttga ttcccaatct tatcccctag
     9181 ttttcctagt ttcggtgaag aaaagacagc ggaaacacca gctatcgaaa caatcaagcc
     9241 gctgacaaaa aggatatttt ctgtacttcc gctcaattca cgaatatata aggtcaaaat
     9301 agggctgatc gtagtgattc ctatctgtaa aatcaaggtc gtaatgaaga gaccgatcaa
     9361 aatagagaga tgatccattt ttgaaaaaat ttctttggtg cttatcagat cttttttctc
     9421 gattggatgg aaatcttctt tgactaagaa aatggtcaat accgttgtga tcatcaacaa
     9481 agcccctgtg atcaagaata cattttccat accaaaccac tgagccagtg caccgcccat
     9541 tgaaggtccg atcagatttc cagcaatcgc tccggtagac aatgttccta acgcccatcc
     9601 gctcttttct ctaggtgcct gtgaagcaat catcgctgtg gcatttggaa tatatccaga
     9661 caaaatccca ttccaaaacc gcatgatcaa aagccaatac gcgtttggta caaaagccaa
     9721 agatcccatt gttaatgtca tccctgctgc tgcacggatc atcatgagtt ttcggccttt
     9781 tcgatcagct agatttcccc agattggtgc aaccacggca gatgcaaaag cagtcacaga
     9841 gattgctagt cccgaaaaca attcgatttg atcttttggt gttcctagtt gctcaacata
     9901 aactggtatg aatggcataa ctaaactaat acttgcacca gtgaaaaaac agccaatcca
     9961 cgcaatcact aaattttttc tccaattaat ctccatctat catccctctt tcgtctcttt
    10021 tatcatacct attttcgaat aaacaagcaa cgatctcgcc agtaaaaaga gaaaaatccc
    10081 gcaaaggtca ttttatatgt aacgttcttt ttatgtaaaa caaaaagaga tcagaacaaa
    10141 tactgttcca atcccttctc tttagttaaa ttgttgctag aatgatgaag ttgataatga
    10201 ataaaatatc aacgatccac aaagcaacag aaacttgatt gtattttcct ttgattactt
    10261 tcacaattgt atagaaaata aatcctgctg caatcccata ggaaatgcta tagcacaatc
    10321 ccataaagat cgatgcaaag aaggccggta aagcttcttc catatctagc catttgatgt
    10381 ctgcaaaaga agccatcatc attactccaa ctaaaatcaa tgctggcgca gttgcttgat
    10441 ttggtacaat ggcgatcagt ggagagaata aactacttaa tgcgaatagc actgccacta
    10501 ctactgaagt caaaccagta cgtccaccgg ccccgatccc agctgcagat tcgacataag
    10561 ttgttgtatt cgatgtacca aagatagccc ccacagaagt agcgatcgca tcggcaaata
    10621 gtgctttatc catttttgtt ttgaacccac gaccatcttc caacgcgatc tcgtcttctt
    10681 ttgagaaaat acctgtgcgg cgtcctgtac cgataaatgt tccgatcgta tcgaaagtat
    10741 cagataaact gaatgcgatg atcgtcatca atacttgagg aatcttggaa gaatcactga
    10801 ataaagattg catgccttca gaaccaaatg ctgcaccgaa agttgttcct aattctttaa
    10861 atgaattacc taacgaattc gtttgccaat caatggaact taaatcaaca acacccataa
    10921 aaataccaag gactgtggtc gctacgatac cgataagaat agccccacgg acatttttta
    10981 caactaaaat ggctgtcagt accaaaccaa tcacagccaa taagatagga gcgttgtcaa
    11041 agttggctaa cgcagggacg attccaccat ttatcgtaac atttgtcgct tttccgcctt
    11101 caacgacaga gctcgaaata gctgactggt ctgctgaaaa agataagaat cctgcatttt
    11161 tgattccgac atacgcaacg aaaataccga tcccgccacc aatcgcatgt tgaagacttt
    11221 cgggaatcgc ccgaataatc atttttcgaa tatttgtgac agtaataaaa atattgatta
    11281 atccgcaaat aaacaccatt gccaaggctt gttgccaaga ataaccaagc ccaaaaacga
    11341 ccgtaaaagt gaaaaatgcg ttcaatccca taccaggtgc ctgagcatat ggtacatttg
    11401 cgaacaatcc cattaccagc gtaccaataa tcgatgcaat gattgtcgct agaaatactg
    11461 cttggaaggg catacctgat gctgacaaaa tcgtcggatt tacgaataag atataactca
    11521 tggcaaagaa tgtcgttact cccgccatta tttcagttga tacatttgta ttgtttttct
    11581 ttaaaccgaa aaacttttcc ataaaaagtt ccactccctg aatttttttc tgatggaaaa
    11641 ctataataat aaaaaataag taaaataggg caaatccatc gaacaatttc aaattatata
    11701 gacgtttcat tcgcttttca acgttttttt aacaaaaaaa gcgaaaataa tacttaacgt
    11761 tcgtgtttag cttatgttta gctatttata tagccataac tgtagttttt gttaggaata
    11821 gctgattgct cttgttaatt acctaaaata tgctagacta aaacaaatgc tagttgagga
    11881 aggaaggacc cttattgaat aaaaaattaa ttgccatcga tctggacgga actaccctca
    11941 atcaagattc gatgattaca tctaaaacaa aagagactct aaaaaaagca atcaatgccg
    12001 gtcatcatgt cagtattgct acaggacgcc cattccgaat gagccatcag ttttaccaac
    12061 aattagaatt aacaacaccg atggttaatt ttaacggcgc attagtacac atccctaatc
    12121 agcactggga tggtgaaaga gaaacgttga tcaatcgtga aatcgctttt gaaatcttat
    12181 cgcaaaaaaa gcagctaaat ttagatttta tcgctgctga aaatcgtgat acttttttta
    12241 ttgacagttt tgatttcttt gatgaaaaaa tttttgcttc ttctcgtcca ggagagaaga
    12301 atttgctttc tcctaaaaat ttgacgacaa atcctacatc tttgcttgtc cgaacagaca
    12361 aacgttttgc aggagctgta tctgctgaat taacacgtca atttggcagt tatgtcgatg
    12421 ttcggacatg gggtggacct actgccatct tagagattgt ttcaaaagga atccaaaaag
    12481 caaaaggcgt gcaagagatt gctaactacc tgtctatcga tcaaaaagac gtcattgcct
    12541 ttggtgacga gcataatgat ttggaattgt tggattatgc aggttgggga gtagctatgg
    12601 ctaacggtac tgaccaactc aaaggcattg caaatgatgt cactcctttg agcaatcagg
    12661 aagacggttt agctgtttat ctggaaaaac tacttaaact ataatagaga attagattcc
    12721 acaaaaaaga ggttgtgaca attttt
//
LOCUS       DAZKLM010000082        12745 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3764
            SAMEA115706913-rid22582563.denovo.085, whole genome shotgun
            sequence.
ACCESSION   DAZKLM010000082 DAZKLM010000000
VERSION     DAZKLM010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706913
            Sequence Read Archive: ERR13196647
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12745)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12745)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287823.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287823.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,004
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,849
            CDSs (with protein)               :: 2,849
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 41 of 96
            Pseudo Genes (incomplete)         :: 52 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 15 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12745
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706913-rid22582563.denovo.085"
                     /isolate="VRE3764"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            44..781
                     /locus_tag="ACG842_002141"
     CDS             44..781
                     /locus_tag="ACG842_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFQ0248659.1"
                     /translation="MAMIKFDHVDKYYGKFHALKNINLEFEKGEVVVVIGPSGSGKST
                     MLRCINGLETISSGKLLINDTDLHDKKTKLTEVRKNIGMVFQHFNLYPNKTVLENIKL
                     APIKVLKQDQATAVKNAEKFLKTVNMLDKKDSYPSMLSGGQQQRVAIARGLAMNPEML
                     LFDEPTSALDPEMIGDVLDVMKKLAHDGMSMIVVTHEMGFAKEVADRVIFMADGEVLE
                     DSRNVRDFFENPNEVRAQQFISKVINH"
     gene            795..1610
                     /locus_tag="ACG842_002142"
     CDS             795..1610
                     /locus_tag="ACG842_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HFQ0248660.1"
                     /translation="MRRLKKIRLIFLLGIAFLALAGCKGNGLANEDILERSKSTNEII
                     WGVKYDTRLFGMMDIESRTVQGFDVDIAKAITKKILGDNGKTEFVEVTSKTRIPLLKN
                     GNIDAIIATMTITDERKKQVDFSDVYFDAGQALLVKKGSQIKSVDDLNASTTVLAVKG
                     STSAANIRQHAPDAKILELENYAEAFTALQSGQGDAMTTDNAILLGIADENPEYELVG
                     GTFTNEPYGIAINKGQENFLKAVNQALEEMHADGTYDKIYQKWFPNETEGKVE"
     gene            1623..2270
                     /locus_tag="ACG842_002143"
     CDS             1623..2270
                     /locus_tag="ACG842_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFQ0248661.1"
                     /translation="MSELIQTYGPIFVDGFKITLFSSVLALVFSLIIGTLMAIFQLSK
                     NKVVSALAKAYVEFFRNIPLLIIVMFFYVVVPIYWFNLDGFQAGTIGLTIYTSAFIAE
                     TVRSGIQTVPKGQTEAGLSSGFTYSEIMRYIVLPQAFKIVIPPLGNQFINLVKNSSIL
                     AIVAGLDLMYQGDLIASETFNTFDTYIIVGLFYLVITLPLSYLMVYLEKKWAVRS"
     gene            2286..2942
                     /locus_tag="ACG842_002144"
     CDS             2286..2942
                     /locus_tag="ACG842_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFQ0248662.1"
                     /translation="MDFSGAFSWMNIRFLLEGLKVTVEVSLFSIIFSFLIGGLTGVIR
                     FANIPFFSKILGVVIDIIRNLPLLLIIFFTYFALPQIGIQMNIFWSAVAALTIFESAM
                     LSEIFRAGLNAVPKGQMEAGLSTGLTYVESMRTIVLPQAFKSMIPAIVSQLISLIKDT
                     SLAVIISLPELSHQARIIYGQNTNYVLPMFIIMTLMYFVVCYALSLLSGYLEKRRYSY
                     "
     gene            3104..3925
                     /gene="racE"
                     /locus_tag="ACG842_002145"
     CDS             3104..3925
                     /gene="racE"
                     /locus_tag="ACG842_002145"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HFQ0248663.1"
                     /translation="MIRLTDNRPIGFIDSGVGGLTVVKEALKQLPNENILFVGDTARC
                     PYGPRPAEQVIQYTWEMTDYLVEQGIKMLVIACNTATAVALEEIKAALSIPVIGVILP
                     GTRAAVKKTQNKQVGIIGTIGTVKSQAYEKALKEKVPELTVTSLACPKFVSVVESNEY
                     HSSVAKKIVAETLAPLTTKKIDTLILGCTHYPLLRPIIQNVMGENVQLIDSGAETVGE
                     VSMLLDYFNLSNSPQNGRTLCQFYTTGSAKLFEEIAEDWLGIGHLNVEHIELGGK"
     gene            3928..5283
                     /gene="rph"
                     /locus_tag="ACG842_002146"
     CDS             3928..5283
                     /gene="rph"
                     /locus_tag="ACG842_002146"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0009022 - tRNA nucleotidyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HFQ0248664.1"
                     /translation="MRHDGRKAQELRKLELQTNVFKYPEGSVVIRFGDTTVICSATIE
                     NSVPPFLRETGTGWVTAEYSMLPRATSTRNRRESSKGKLSGRTMEIQRLIGRSLRAVV
                     DLEKLGERSIIVDCDVIQADGGTRTASITGAFVALKLAIEKLLREKELSEDPIKEHLA
                     AVSVGILPDGTCVTDLDYQEDSAALVDMNLVMTESGKFVEIQGTGEEATFDGEQLNEM
                     LFFGKNAIEDLIKEQKHALLTEFAQNDERIEETKTIIIATRNPGKAEEFRNMFKEAGY
                     HVKTLLDYPELPDVEETGSTFEENARLKAETIAQLLDQPVLADDSGLKVDALGGMPGI
                     YSARFAGEQKSDAGNNAKLLYELTDVPDEKRTAQFHCTLVFAAPKKDSLVVEAEWPGR
                     VARIPSGENGFGYDPLFIPEGKKQTAAELSSEEKNKISHRAQAMKKLSAEWKQWLEGE
                     R"
     gene            5284..5802
                     /locus_tag="ACG842_002147"
     CDS             5284..5802
                     /locus_tag="ACG842_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFQ0248665.1"
                     /translation="MRYLVVSDNHGDRDILVDLVDRYRSEVDIFFHCGDSELEASDPL
                     WDTFQVVQGNCDYGPGFEQKKVIQTGQDTVFMTHGHLSNVRFGLTQLAIEARAATADM
                     VFFGHTHQIGCEMDQKILFLNPGSISQPRGPIQIPSYALIESLDDQISVQYYNRAHQA
                     VEELAFSFSRER"
     gene            5909..6409
                     /gene="cbpB"
                     /locus_tag="ACG842_002148"
     CDS             5909..6409
                     /gene="cbpB"
                     /locus_tag="ACG842_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0180001 - cyclic-di-AMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP-binding protein CbpB"
                     /protein_id="HFQ0248666.1"
                     /translation="MIGPIVREMLLENQETFLIPAENVANVMYHHPLSHGLLVLSKVG
                     YSKIPVLGKDDHFVGLVSLSDVVNKMIDLQTISMDPLEGLTVADVTETDVPTIDENWE
                     LEEVLHLLVDSPFLPVVTEDRIFKGIITRKELLKAVNFMVHELERRNILSPKIEELKE
                     QIDIVS"
     gene            complement(6689..7609)
                     /locus_tag="ACG842_002149"
     CDS             complement(6689..7609)
                     /locus_tag="ACG842_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HFQ0248667.1"
                     /translation="MFSLLATAGTTDSSGETLTDQAVSKVNAFQRFWDGIDWDAILAA
                     FIQKSLYLIFLIVLFWILHRVGKYLIDKSFNQYSKKAILNESRLKTLHSLLNTIFHYT
                     LGFFFIYAILSAIGVPIGSLLAGAGIAGVAIGLGAQGFMSDVITGFFIIMEQQMDVGD
                     YIKLANLTIEGTVVSVGIRTLQLKATDGTVHFIPNRNITTISNLSRANMQVLLDIRIV
                     PEEGYDKIYEIIDRVNRTLAEKYQEELQTEPSIFGLVDLGNSNFAIRTVCYALNGKQY
                     ALKEEFLSSYVKELTASGFTIPNSPIAVGK"
     gene            7735..8787
                     /locus_tag="ACG842_002150"
     CDS             7735..8787
                     /locus_tag="ACG842_002150"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HFQ0248668.1"
                     /translation="MIKCGENNEGGKYMQIDWNNLGFSYIKTPWRFIAKWKDGQWEEG
                     ELTEDNYLTIHEGSPALHYGQQCFEGLKAYRRKDGKVNLFRPEQNSRRMNQSARRLLM
                     PMVPEKMFVDAVKQVVKANEDFVPPYGTGATLYLRPLLIGVGENIGVHPAPEYLFVVF
                     CTPVGAYFKGGLKPTNFIVSDYDRAAPKGTGAAKVGGNYAASLLPGTEAKELDYSDCV
                     YLDPATHTKIEEVGAANFFGITKDGKFITPQSPSILPSITKYSLLQLAEERLGLQAIE
                     GDIFIDDLDQFAEAGACGTAAVISPIGGIYHNNNLHVFYSETEVGPVTKRLYDELTGI
                     QFGDIEAPEGWIEVVE"
     gene            complement(8897..9652)
                     /gene="fabI"
                     /locus_tag="ACG842_002151"
     CDS             complement(8897..9652)
                     /gene="fabI"
                     /locus_tag="ACG842_002151"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HFQ0248669.1"
                     /translation="MSFLEGKKIVIMGVANKKSIAWGCAEAMLKQGAEIIYTYQNERM
                     KKSLVKLVGEEAFTVECDVSSDESIEKAMNAIGEYAGEIHGLVHAVAYANKEELSGNV
                     SDISRDGYQLAQDISSYSLIAVSKYAQPFLAPNSGIVSMTYLGSERAIPNYNMMGIAK
                     ASLEAAIRYLAAEFSPKGIRVNGISAGAIKTLAVTGVKDYQKLIQLSESRTPDQQGVT
                     IEEVGNACAFLISPLSSGIIGDIIYVDKGVHLS"
     gene            9872..10312
                     /gene="fabZ"
                     /locus_tag="ACG842_002152"
     CDS             9872..10312
                     /gene="fabZ"
                     /locus_tag="ACG842_002152"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HFQ0248670.1"
                     /translation="MSYVLSATEVMELIPNRYPICYIDYVDTLEPGKKIVATKNVTIN
                     EDFFQGHFPDNPVMPGALILETLAQAGSILILKSEEFLGKRAYIGGINKAKFRQKVVP
                     GDVLKCHFEITKMKGSVGTALSAAYVDDKKVCECEFTFIVNQNA"
     gene            complement(10427..11188)
                     /locus_tag="ACG842_002153"
     CDS             complement(10427..11188)
                     /locus_tag="ACG842_002153"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFQ0248671.1"
                     /translation="MKKNKVILSISLAAALLFAAGCSANNTTSSSSSTESSKSTSTAE
                     STTASSVDLNALELPQLTKDVAENEDLVEMVTNKGTIKIKLFPEHAPKAVENFMTHAK
                     DGYYNGLTFHRVIKDFMIQGGDPKGDGTGGESIWGEGFETEINNHLYNIRGALSMARS
                     QDPNSNGSQFFIVQNTDDMHDGLLKDDYPQAIIDAYKNGGYPSLDGHYTVFGQVIEGM
                     DVVDAIANIETDSSDKPKEEVKIDKINILQEAKQK"
     gene            11377..12471
                     /locus_tag="ACG842_002154"
     CDS             11377..12471
                     /locus_tag="ACG842_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HFQ0248672.1"
                     /translation="MGKLGISIYPERSTFEKDKAYLDLAHKYGYKRVFTSLLQINDDK
                     EKVLSEFKEVVDYANSLGMEVMVDINPALFEQLEISYDDLSFFHKMGAYGVRLDIGFT
                     GAEEAKMTRNPFGIKIEINMSSGTNYVDNIMSYSPNTDNLLGSHNFYPHRYSGLGYEH
                     FVFCSEKFRKYNLNTMAFVNSQSAEFGPWPTQDGLCTLEDHRDLEIATQVKHLILTGL
                     IDDISIGNAYASEEELKEMAEAFNADYPTLKVDTEEGITENERICLFDNLHSYRGDRS
                     EYILRSTMTRVYYKDKDFPPHNTRDMHHGDVLIDNEGYGQYKGETQIALKDMKNDGRV
                     NVVGRISDDELFLLDFLKPWSSFKLIENKK"
     gene            complement(12557..>12745)
                     /locus_tag="ACG842_002155"
     CDS             complement(12557..>12745)
                     /locus_tag="ACG842_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFQ0248673.1"
                     /translation="NSNGILRRNGLPKSMDFRKVNQTFISSVSNQRNHIPRKSLNYRT
                     PIEIFLSYVQEAFYSSLI"
BASE COUNT         4105 a         2207 c         2676 g         3757 t
ORIGIN      
        1 ctttaaaaga aatcttacta aataataatg ggagtgtaat tctatggcaa tgattaaatt
       61 tgatcacgtg gataaatatt atggcaagtt ccacgcatta aaaaatatca atctcgaatt
      121 cgagaaagga gaagttgtcg ttgtcattgg tccttctggt tcaggaaaga gcacgatgct
      181 tcgctgtatc aatggattag agacgatctc ttcaggtaag ctgctgatta atgatactga
      241 cttgcatgat aaaaagacaa aactgacaga agtccgaaaa aacatcggca tggttttcca
      301 acatttcaat ttgtacccaa ataaaactgt tctggaaaac atcaaattag caccaatcaa
      361 agtgttgaag caagaccagg caactgcagt caaaaacgcg gagaaatttt tgaaaactgt
      421 caatatgtta gataagaaag attcttatcc ttccatgtta tccgggggac aacagcaacg
      481 ggtggcaatc gccagaggtt tagcgatgaa tcctgagatg ctgctgtttg atgaacctac
      541 ttctgctttg gatccggaaa tgattggtga tgtattagac gttatgaaaa aattggctca
      601 tgacggtatg tcaatgattg tcgtgacaca tgaaatgggc tttgccaaag aagtggcgga
      661 tcgtgttatc ttcatggctg atggagaagt attggaagac agcagaaatg tgagagactt
      721 tttcgaaaat ccaaatgaag tccgagcaca gcaatttatc agtaaagtta tcaatcattg
      781 ataggaggaa aaagatgcgc aggttaaaaa aaatacgcct gattttcctt ttaggcattg
      841 cttttcttgc actagccgga tgtaaaggaa atgggttggc aaatgaagat attttagagc
      901 gaagcaaatc taccaatgaa atcatctggg gtgtcaagta tgatactcga ttatttggaa
      961 tgatggatat tgagagcaga actgtacaag gatttgatgt agatattgca aaagcaatca
     1021 ctaaaaagat ccttggtgac aatggaaaaa cagaatttgt tgaagtcact tctaaaactc
     1081 ggattccatt attgaaaaac ggaaatatcg atgcaattat tgcaacgatg acgatcacag
     1141 atgaacgaaa aaaacaggtt gacttttcag atgtctattt cgatgctggc caagctttgt
     1201 tggtaaagaa aggaagccaa atcaaaagtg tagatgattt gaatgcttct acaacggttc
     1261 tagcagtcaa aggttctaca tcggctgcga atatccgaca gcatgcacca gatgcaaaaa
     1321 ttttagaatt agaaaactat gcagaagcat ttactgcttt acaatctggt cagggagacg
     1381 cgatgactac ggataatgcg attttgttag ggattgcaga tgaaaatcca gagtatgagt
     1441 tagttggtgg aacgtttact aatgagcctt acgggattgc gatcaacaaa gggcaagaaa
     1501 acttcttgaa agctgttaac caagcgctag aggaaatgca tgcagatggc acatacgata
     1561 aaatctatca aaaatggttt ccaaatgaaa ctgaaggaaa agtagagtaa gggggagagg
     1621 caatgagcga attgatacaa acttatggac caatctttgt cgacgggttc aagatcacat
     1681 tgttttcgag tgtcttagca cttgtgttta gtttgattat cggaacgtta atggcaatct
     1741 tccaattatc aaagaataaa gtagtgagcg ccttagcaaa ggcatatgta gaatttttta
     1801 gaaacattcc attattaatc atcgtgatgt ttttttatgt tgtcgtaccg atttattggt
     1861 ttaatctaga tggattccaa gcaggtacga ttggattaac gatctataca tctgctttta
     1921 ttgctgaaac agtacgttca gggattcaaa ccgtaccaaa aggtcaaaca gaagcaggac
     1981 tatcttctgg ttttacctat tcagaaatca tgcgttatat tgttttaccg caagcattca
     2041 aaatcgttat cccgccatta ggcaaccaat ttatcaacct cgtaaaaaac tcatctattt
     2101 tagcaatcgt agctggttta gatttgatgt atcaaggaga cttgatcgct agtgaaacat
     2161 tcaatacctt cgatacgtat atcatcgttg ggttatttta cttagttatc acgctaccgt
     2221 tatcttactt aatggtttat cttgagaaaa aatgggcggt tcgttcatag aaggagggga
     2281 aatacatgga ttttagcggc gctttttcat ggatgaatat ccgtttttta ttggaaggat
     2341 taaaggttac tgttgaagtt tcattgtttt cgatcatttt tagtttcttg atcggtggtc
     2401 tgacaggtgt gattcgtttt gcgaatatcc ctttcttttc caaaatcctt ggtgtagtga
     2461 ttgatattat acggaatctt ccattattat tgatcatttt cttcacttac tttgccttac
     2521 ctcaaatcgg cattcagatg aacatcttct ggtccgctgt cgcagcatta acgatttttg
     2581 agtcagctat gttgtcggaa attttccgag ctggactcaa tgctgttcca aaaggacaga
     2641 tggaagcagg attatcaacg ggactgactt atgtagaatc gatgcgaacg atcgtgttac
     2701 cgcaagcatt taaatcaatg atcccggcaa ttgtgagcca attgatttca ctgatcaaag
     2761 atacttcatt agcagtaatc atttctttac ctgaactttc tcatcaggca cgtattattt
     2821 atggacagaa caccaattat gtgcttccga tgttcatcat catgacactt atgtacttcg
     2881 tggtatgtta cgcgctttct ctgctttccg gttacttgga aaagagacgg tacagttatt
     2941 aatagatgat tcttttagcg gttgtggcaa actaaagtca caaccgcttt tcctctttcc
     3001 attcgaaaaa aagagatttc gattgtttta agcaagtaag agcacttgaa aattcacgct
     3061 aaaaagtaca caatagggag gatagaattt attgaaaaaa aggatgatac gattgacaga
     3121 taatcgccct atcggattta ttgattcagg tgtcggcggc ttgactgtag taaaagaagc
     3181 cctgaaacaa ttaccgaatg aaaatatttt atttgtagga gacacagcac gctgcccata
     3241 tggccctaga cccgcggaac aggtaatcca gtatacttgg gaaatgacgg attatctggt
     3301 ggagcaagga atcaagatgc tggtgatcgc ctgcaatacc gcaactgcgg tggctttaga
     3361 agaaatcaaa gctgctcttt ctattccagt catcggtgtg atccttcccg gtactagagc
     3421 ggcagtaaaa aaaacacaaa ataaacaagt tggcattatc ggtacgattg gtacggtaaa
     3481 aagtcaagct tatgaaaaag cactgaaaga gaaagtacca gaattgactg tgacaagtct
     3541 tgcttgtccc aaatttgttt cagttgtcga aagtaatgaa taccattcat cggtggcgaa
     3601 aaaaattgtg gcagaaacat tagctccttt aaccactaaa aaaatcgata cattgatttt
     3661 gggatgcacc cattatccat tattacgccc catcattcaa aatgtaatgg gagaaaatgt
     3721 tcaactgatc gattctggag cagaaacagt aggtgaagta tctatgctgt tagattattt
     3781 caatttgagc aattcaccgc aaaatggtcg gacattatgc cagttttata caactggctc
     3841 tgccaaactt ttcgaggaaa tagctgaaga ctggcttgga atcggacact taaatgtaga
     3901 acatatcgaa ttgggaggaa aataaggatg cgtcatgacg ggagaaaagc ccaagagctt
     3961 cgcaaactgg agcttcaaac aaatgttttc aaatatccag aaggatcggt agtgatccgt
     4021 tttggtgata caacagttat ttgttctgca acaatagaaa attctgttcc cccttttttg
     4081 agagaaacag gaacaggatg ggtgacagca gaatatagta tgctgcctcg agcaacgagt
     4141 acgagaaatc gtagagaaag cagcaaaggg aaactttctg gtagaacaat ggaaatccag
     4201 cgtcttattg gtcgttcctt gcgtgcagta gtagacttgg aaaaattagg agaacgtagt
     4261 atcatcgtag actgcgatgt gatccaagcg gatggcggta ctagaacagc aagtatcact
     4321 ggggcatttg tggcattgaa attagccatt gaaaaacttt taagagaaaa agaattgtca
     4381 gaagatccaa taaaagaaca tcttgctgca gttagcgtag gaatcttacc agatgggact
     4441 tgcgtcacag atttggatta ccaagaagat tccgcagcac ttgtagacat gaatcttgta
     4501 atgacagaat ctggaaaatt cgtggagatt caaggaactg gagaagaagc aacgtttgat
     4561 ggtgaacagt tgaatgaaat gctctttttt ggaaaaaacg cgattgaaga tttgatcaaa
     4621 gagcaaaaac atgctttgct aacagagttt gctcaaaatg atgagcggat agaagaaaca
     4681 aaaacaatca tcattgcgac aagaaatcca ggaaaagcag aagagtttcg aaatatgttc
     4741 aaagaagcgg gatatcatgt aaaaacatta cttgactatc ctgaattgcc agatgtggaa
     4801 gaaactggca gcacatttga agaaaatgcc cgattaaaag cagaaacgat tgcacaattg
     4861 cttgatcagc cagtcttagc agatgattca ggtttgaaag tggatgcttt aggcggtatg
     4921 ccaggaatct attctgcacg ttttgcaggg gaacaaaaaa gtgatgcggg gaataacgcc
     4981 aaattattat atgaattgac agatgtgccg gatgaaaaaa gaacagcaca attccattgt
     5041 acgttagtat tcgcggcacc caaaaaagac agccttgtag tagaagcgga atggcccggt
     5101 cgagtagccc gtataccatc tggcgaaaat ggctttgggt atgatccttt atttatccca
     5161 gaaggtaaga aacaaacagc agcagaatta tcgagcgaag aaaaaaacaa gatcagtcat
     5221 cgggctcaag caatgaaaaa attgagtgcc gagtggaaac aatggctgga aggagagaga
     5281 tagatgcggt atttagtagt aagtgataat catggagaca gagatatcct cgttgactta
     5341 gtagatcgtt atcgttctga agtggatatc tttttccatt gtggtgattc ggaattagaa
     5401 gcttctgacc cattatggga tacattccaa gtggttcaag gtaattgtga ttatggacca
     5461 ggattcgaac agaaaaaagt gatccaaact ggtcaggata cagtattcat gactcacggt
     5521 catttatcga atgtccgatt tggtttgaca cagttagcaa tcgaagctcg agctgcaact
     5581 gccgatatgg tgttttttgg tcatacccat caaataggct gtgagatgga tcagaaaatc
     5641 ctgtttttga atcctggtag catttctcag cctagaggcc cgatccaaat cccatcatat
     5701 gctcttatcg aaagtttaga tgaccaaatt tctgttcaat actataaccg tgcccatcag
     5761 gcagttgaag agttggcctt ttctttttcg agagagcgct gaataaaaaa actctaattt
     5821 ttaataaaaa taagctttat tttatgaatt ttgcatccgg tttcggtaaa atagtagtgt
     5881 tgaatgaaat agaaatggag gccatcagat gattggacct atagtaagag aaatgctgct
     5941 ggaaaatcag gaaacgtttt tgattcctgc agaaaatgtg gcaaatgtta tgtatcatca
     6001 tccattgtcc catggtttac ttgtgttatc gaaggtcggt tattccaaaa ttcctgtatt
     6061 aggaaaagat gatcactttg taggactagt aagtttatca gatgtggtca ataaaatgat
     6121 tgacttgcaa acaatcagca tggacccgtt agaaggacta acggttgctg atgtgacaga
     6181 aacagatgtt ccgacaattg atgaaaattg ggaactagaa gaagtactcc atttgttagt
     6241 cgattcgcct tttttaccgg ttgtcacaga agatcggatt ttcaaaggaa tcattacaag
     6301 aaaagagctg cttaaagccg taaacttcat ggtgcatgaa ttagaacgtc gtaatatatt
     6361 gtctccgaaa atcgaagaat taaaagaaca aatcgatatc gtgagctaaa gaacgagaaa
     6421 ctaaaagata atcaaaaaat aagtagaaaa aacagcgtac cagttttgac tttatcttga
     6481 aaaagaggct gggacaaaag cgtttaactc caagaaataa gaaggaattc acgaaaattg
     6541 cttttcaaat ttttgtgaat ttcagcttat ttccgaagga gttgcttctg cttccgccgt
     6601 ttatacgtgt ttagagcgtg ggacaaaagt gttctttact tttgtcccac gctcttttta
     6661 gtgagattcg ttgcatcgtt tgtccaaatt attttccgac agcaattgga ctgttcggaa
     6721 tcgtgaaacc agaagctgtc aattctttga cataggagct caagaactct tcttttaagg
     6781 catattgttt tccgttcaat gcgtagcaga ccgtacgaat cgcaaaattg ctgtttccaa
     6841 gatccactaa tccaaagata gaaggttcag tttggagctc ttcttggtat ttttcagcta
     6901 atgtacgatt tactcgatca atgatctcat agattttgtc atatccttct tcaggaacaa
     6961 tccgaatatc taaaagtact tgcatatttg cacgagagag attgctgatc gtggtgatgt
     7021 ttcgattcgg gatgaaatgg acagtaccat ccgttgcttt cagctgaagg gtacggatac
     7081 caacagatac gacggttcct tcaatcgtta agtttgctag cttgatgtag tccccaacat
     7141 ccatctgttg ttccatgata ataaaaaatc cggtaataac atcactcatg aacccttggg
     7201 caccaagacc gattgccaca cctgcaattc cggcaccagc caaaagtgat ccgatcggca
     7261 caccgatagc agataaaatt gcatagataa aaaagaaacc aagggtataa tgaaaaatag
     7321 tatttaaaag actatgtaaa gtctttaagc ggctctcgtt tagaatggct tttttagaat
     7381 actgattaaa agacttatca atcagatatt ttcccacacg gtgaagaatc caaaaaagga
     7441 cgatcaaaaa aatcagatat aaactttttt ggataaaggc agccaaaatg gcgtcccaat
     7501 caatcccgtc ccaaaaccgc tgaaacgcat ttactttgct gacagcttga tctgtcaatg
     7561 tctcgccaga agagtctgtc gtaccggcag ttgctaataa tgaaaacata aaatcctctt
     7621 ttctaaaatt tgattctttc tgtattgtac caagaagttt tcaaaaaagg aaatagcaaa
     7681 aataactata atgaaggaaa aaagctgaaa ataaaatgac aaataatatg gataatgata
     7741 aaatgtggag aaaataacga aggtggtaag tatatgcaaa tcgattggaa caatctaggt
     7801 ttttcttata tcaaaactcc ttggcgtttt attgccaaat ggaaagacgg acaatgggaa
     7861 gaaggagaat tgacagaaga taattacctg acgattcatg aaggctctcc agcgcttcat
     7921 tatggtcagc aatgttttga aggtctaaaa gcttatcgtc gaaaagacgg caaggtcaat
     7981 ctctttcgac ctgaacaaaa tagtcgacgg atgaatcaaa gtgctagacg attgctgatg
     8041 ccaatggtcc ctgaaaagat gttcgttgat gctgtcaagc aagtggtaaa agcaaatgaa
     8101 gattttgtgc cgccctatgg aacaggcgct acattatact tgcgcccttt actgattggg
     8161 gtcggtgaaa acattggtgt ccatccagct ccagaatatt tatttgtcgt cttctgtacg
     8221 ccagtaggag cgtatttcaa aggtggacta aaaccaacga attttattgt atctgattac
     8281 gatcgtgctg ctcctaaggg gactggtgca gcaaaagtcg gcggaaatta cgcagcgagt
     8341 ttacttccag gaacagaagc aaaagaactg gattactcag attgtgttta tcttgatcca
     8401 gccacacata cgaaaatcga agaagtaggt gcagcaaatt tctttggaat tacaaaagac
     8461 ggaaaattca tcactccgca atcgccctct atcttaccaa gtatcacgaa atattccttg
     8521 ttgcagttag ctgaagaacg actaggactg caagcaattg aaggagatat tttcattgat
     8581 gatctagatc aatttgcaga agctggtgca tgtggtacag cggctgttat ttctccaatc
     8641 ggtgggattt atcacaataa caatcttcat gtattttata gtgaaacgga agtaggacca
     8701 gtaacaaaac ggttgtatga tgaattgaca ggtattcaat ttggagacat agaagcacca
     8761 gaaggctgga ttgaagtcgt ggaataattt caaagtaatg agttgaaaaa aacgtaaaaa
     8821 gaaaaaatca ggaagagact ttaaaaagtt ttttcctgat ttttatatgt tttactttaa
     8881 agaatgcaac aaaagtttat gaaagatgga cacctttgtc aacgtagata atatcaccga
     8941 tgattccgct ggaaagtggg ctgattaaga aagcacaagc attaccgact tcttcgatcg
     9001 tcacaccttg ctgatcaggc gtacgagatt cagataactg gatcagtttt tgatagtctt
     9061 taacacctgt gacagctaat gttttgattg ccccagctga gattccattg acccgaattc
     9121 ctttaggcga gaactcagct gccaaataac ggatagcagc ttcaagagaa gctttcgcaa
     9181 tacccatcat gttataattc gggattgcgc gttctgaacc tagataagtc atgctaacga
     9241 tcccgctgtt tggtgcaaga aacggttgtg cgtattttga tacagcaatt aaagagtagc
     9301 tgctgatatc ttgagctaat tgatagccgt ctctggaaat atcagacaca tttccggaca
     9361 attcttcttt gttggcgtaa gcaactgcat ggacaaggcc gtggatctcc ccagcatatt
     9421 caccgatggc attcattgct ttttcgatgc tttcgtcgct agaaacatca cattctacag
     9481 tgaatgcttc ttcgccgacc aatttgacta atgatttttt cattcgttca ttttggtaag
     9541 tatagataat ttctgcccct tgtttcaaca ttgcttcagc acaaccccaa gcaatgcttt
     9601 ttttgttagc gacgcccata atgacgattt tttttccttc tagaaatgac ataatgactc
     9661 tcctttgttt ctgataaaaa tttgatagtc gtaaaaaaat agagaataat agctaaaata
     9721 acttcttgat ttcttaatta ttagtcaaaa tgttttgatt gtcaaactat tttttcttaa
     9781 attaaaaaaa gattgctaaa tatttaggaa tatgatactt tgatagtcaa agtaatgaaa
     9841 atggcaattg cctgaaagaa ggagtttaat tatgtcttac gtattatctg ctactgaagt
     9901 aatggaattg attcctaatc gttatcctat ttgttatatt gattatgtag atactttaga
     9961 accaggaaaa aagatcgttg cgacaaaaaa tgtaacaatc aatgaagatt ttttccaagg
    10021 tcactttcct gacaatccag tgatgccggg tgcgttgatt ctagaaacac tggcacaagc
    10081 aggttcgata cttatcctaa aatcagaaga gtttctagga aaaagagctt atatcggtgg
    10141 gatcaataag gccaaattcc gtcaaaaagt tgttccagga gatgtgctga aatgtcattt
    10201 tgaaatcacc aaaatgaagg gatcagtcgg tacagcgtta tctgcagcat atgtagatga
    10261 taaaaaagtc tgtgagtgcg agttcacgtt catcgtcaac caaaatgctt gactataaaa
    10321 cctgatatag caaaacaaaa ggaagcttac ttgcttttta tgtaaaaaga agacgcctga
    10381 atcctagcat acgttgtcat ctatgcagga ttcagacgtt atttttttat ttttgttttg
    10441 cttcttgcag tatattgatt ttatcgattt ttacttcttc ttttggtttg tctgaactat
    10501 ctgtttcgat atttgcaata gcatccacga catccattcc ttcaatcact tggccgaaga
    10561 cagtataatg gccatctagt gaaggataac cgccgttttt ataagcgtcg atgattgctt
    10621 gaggatagtc gtcttttaaa agaccatcat gcatatcatc tgtattttga acgataaaga
    10681 actggctgcc gttactgtta ggatcttgtg agcgagccat agataacgca cctcttatat
    10741 tataaaggtg gttgttgatc tctgtttcaa atccttctcc ccaaatgctt tcccctccag
    10801 tgccatcgcc tttaggatcg ccaccttgga tcatgaaatc tttgatgacg cgatggaaag
    10861 tcagtccgtt gtagtaaccg tcttttgcat gtgtcataaa gttttcaacc gcttttggtg
    10921 cgtgttcggg aaataatttg attttgattg tgcctttatt cgtcaccatt tcgaccagat
    10981 cttcattttc ggcgacatct ttcgtcaatt gtggtaattc caatgcgttc aaatcgacgc
    11041 tactggcagt cgtgctttcc gcagtagatg tactttttga actttctgta gacgaactag
    11101 aagaagtcgt gttgtttgca gaacaaccag cagcaaacag caatgctgct gctaaactaa
    11161 tacttaaaat aactttattt ttcttcataa ctgttccttt ccaatttact attttgttat
    11221 ctttaatgat aacaaaatag agggaaaata cctacttaaa ttattggaat ttatcattta
    11281 ttttaaaaga aaaagaaaag gtgtagacca tttactttgt gtttgctata atgtaagtgg
    11341 atacaaaaac gaaaagatgc aggaggaaat ggtaatatgg gaaaattagg aatctcgatt
    11401 tatccagaac ggtcaacatt tgaaaaggac aaagcgtact tggatttagc tcataagtat
    11461 gggtataaac gggtttttac cagtttgctg cagatcaatg atgataaaga aaaagttctt
    11521 tcagaattta aagaagtagt tgattacgcg aatagcctag gcatggaagt aatggttgat
    11581 atcaatccag cgttatttga acagttggag atttcttacg atgacttatc ttttttccat
    11641 aagatgggtg cctacggggt ccgcttagat atcggattca caggagcaga agaagcaaaa
    11701 atgacgcgca atccttttgg tatcaaaatc gagatcaata tgagttcggg cacgaattac
    11761 gttgacaaca tcatgagtta ttcgccaaac acggataatt tattaggttc tcataatttt
    11821 tatcctcatc gatactctgg tttaggatac gaacactttg ttttttgttc agaaaaattc
    11881 agaaaatata atttgaatac gatggcattc gtcaattcgc aatctgctga gtttggccct
    11941 tggcctacgc aagatggctt gtgcacgctg gaagaccacc gagatcttga aattgcgaca
    12001 caagtgaagc atttaatcct tacaggattg attgatgata tttctattgg aaatgcgtac
    12061 gcaagtgaag aagaattgaa agaaatggca gaagcattta acgcggatta tccaacattg
    12121 aaagtagata cggaagaagg tattacagaa aacgaacgaa tctgcttgtt tgataacctt
    12181 catagttatc gaggggatcg ctcagaatat atccttcgtt caacgatgac tcgtgtatac
    12241 tacaaagata aagatttccc gcctcataat acgcgagata tgcatcatgg ggatgtactg
    12301 attgacaatg aagggtacgg tcaatacaaa ggagaaacac aaatcgcctt gaaggatatg
    12361 aaaaacgacg gacgtgtcaa tgttgtagga agaatctccg atgacgaatt attcctcctt
    12421 gactttttga aaccatggtc aagctttaaa ttaattgaaa ataaaaaata gataattagt
    12481 tattgcagaa ataatccgct agaaatagcc agttatttct gcttttttat tttgtttttt
    12541 tataggttat gatttgtcaa attaagctag aataaaatgc ttcttgtaca tagctcaaaa
    12601 atatttcgat tggtgttctg taattcaatg attttcttgg aatatgatta cgttgattgc
    12661 tgacactgga aataaatgtc tgattcactt ttctaaaatc cattgatttc ggcagtccat
    12721 tacgacgcag aatcccgtta gaatt
//
LOCUS       DAZKLM010000083        12339 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3764
            SAMEA115706913-rid22582563.denovo.086, whole genome shotgun
            sequence.
ACCESSION   DAZKLM010000083 DAZKLM010000000
VERSION     DAZKLM010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706913
            Sequence Read Archive: ERR13196647
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12339)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12339)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287823.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287823.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,004
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,849
            CDSs (with protein)               :: 2,849
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 41 of 96
            Pseudo Genes (incomplete)         :: 52 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 15 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12339
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706913-rid22582563.denovo.086"
                     /isolate="VRE3764"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..154
                     /locus_tag="ACG842_002156"
     CDS             <1..154
                     /locus_tag="ACG842_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HFQ0248674.1"
                     /translation="SLEKLGCQNFNSRSVKGLESRFESILLAYSVLLSRAQRRFEGTL
                     RYSLGY"
     gene            complement(208..486)
                     /locus_tag="ACG842_002157"
     CDS             complement(208..486)
                     /locus_tag="ACG842_002157"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0248675.1"
                     /translation="MENKVGYVRYNFFSSTPIMKDFASFNEALAVELSHDRERIHYEK
                     HVIINDLWNTEKTDLLALPDTDYPVFKEIEVKANKYNEIKQNKITNLS"
     gene            complement(601..1218)
                     /locus_tag="ACG842_002158"
     CDS             complement(601..1218)
                     /locus_tag="ACG842_002158"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0248676.1"
                     /translation="MLTMTDINTIRNLRNNHDKSINHIAKELDVNWRTAKKYADDDLL
                     PQPKARKKTGMMYTEKWGGIVSLWLSEDAKLPNKKRRTAEAMTTALKADGFPGSYRTV
                     CVFIQDWKATHYLETLEDQGFERLEHPPAEAQLDFGTMEVCHDGAFKDVKVLVMTFPF
                     SNAGFAVVLPAENQECLLEGMKELFSQSGGVPRKIRIDNMSTAVT"
     gene            complement(1613..2437)
                     /locus_tag="ACG842_002159"
     CDS             complement(1613..2437)
                     /locus_tag="ACG842_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFQ0248677.1"
                     /translation="MEIKVLFENSRINLNFKSGHGLSLLINYSGKKYLFDAGANKKLV
                     YNATKMGETLNDLEAVIISHGHSDHTGGLRYLKSGNAPIYTAEGVTLPHYLKILTTFL
                     YVGIPKNEILKMHENLHTIKTKTELAPSFHIIPLGKANSTTKNLYKKMDDKFVYDDFS
                     DELLVVIEQEDGLIVFTGCSHHGIIDMMEIALETFPTKPIKYLIGGFHMIGIPYINNL
                     GLPEQEVMKIGNKLNDSRVEKILTCHCTGSKAFNILKTQLGNKLEAIKTGQHLEIS"
     gene            2780..3196
                     /locus_tag="ACG842_002160"
     CDS             2780..3196
                     /locus_tag="ACG842_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFQ0248678.1"
                     /translation="MATIILASHGEFAQGLKQTATMIIGDSVPIHALSAFRDEDESIL
                     VQIKKLLATIDIEETYILTDILGGSVNNDMLTIIKEYEGLQLITGMNLPLVISIATYN
                     GKIGESELEKLIKESQQSVIDCRKLLVEQLTGGDDL"
     gene            3193..3663
                     /locus_tag="ACG842_002161"
     CDS             3193..3663
                     /locus_tag="ACG842_002161"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFQ0248679.1"
                     /translation="MIKLVRIDHRLLHGQVVFSWSKSLNINRIIIANKEAANDEFKKM
                     SLNLSKPTGIKLTIFSVEQLLEKMPKIETLNENIMLIFGNTKEIRQFCEAYPKVQEIN
                     YGGIIKKEGSKQFSNAIFLNESEQEDAKKLKALGIKQFIQQVPTSKKEDLNTML"
     gene            3677..4450
                     /locus_tag="ACG842_002162"
     CDS             3677..4450
                     /locus_tag="ACG842_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFQ0248680.1"
                     /translation="MLVQSIGLGLIGVFCILDSRLLGRMNFERPLIVSTLVGLVLGDM
                     EKGLIVGASLELMSLGLVNIGAASPPDMNMASIIATAFAILSNANAEAALTIAIPISV
                     LGQILGILMRTILSNLTHVADRFIEQGKFSQARRIHILYGSGLYALMYFIPIFLAIYF
                     GTNLVSQIVDAIPEWLTNGLTVASKILPAYGFALLLQTMLTKKMVPFLLLGFLITAYS
                     GLGIIGIAAFACIVAFVMVQVQGKNSNGNQNEPDPLDDL"
     gene            4464..5276
                     /locus_tag="ACG842_002163"
     CDS             4464..5276
                     /locus_tag="ACG842_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFQ0248681.1"
                     /translation="MKEKDQNAWKYRQFLWRSWAIQASWNYERQMNMGFMYGIAPILD
                     NIYKNPEDEAMKKEAYERHMMYYNCTPQTSAFVLGLTASMEEQYYEDQENFNPESISA
                     VKTSLMGPLSGVGDSFFQGTIRVLAFGLGINLAQQGSILGPILAMLISFVPSFLVTYY
                     GGKIGYNMGNKYLSKLYQEGLMDKVMYVCSIVGLMVIGSMIASMIGITTPIQMGKAFV
                     LQDVLDSIMPQFLPLGLTFFMYWLLQKKVKTGWMLAICLVGGIIFSLLGILQ"
     gene            5330..6295
                     /locus_tag="ACG842_002164"
     CDS             5330..6295
                     /locus_tag="ACG842_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFQ0248682.1"
                     /translation="MNSKEIVKEIVAKKEVKSIVFVGCGASKADLYPAKYFLDQNAKQ
                     LRISHYTASEFNYATPASVDESTVVISASLGGTTPETVEANAVAKEKGATVISLTRSI
                     DSPLTKDADYVIYHRFAENYAAKLEKTGYCLELAVELLHQVEGYDHYEAMQEGFATIY
                     DLAQKSAESARKDAKKFAERFKDEKVIYVMSSGATHEVAYSTSICLMMEMQWINSGTF
                     HSGEFFHGPFEIVDKDVPFILLMNDGKTRNIDARALTFLERFDTKTEVVDALDYGLSS
                     HIAKEVLDYFNPFVITAVFRVYAEELSEAREHPLTKRRYMWKLTY"
     gene            6431..9154
                     /locus_tag="ACG842_002165"
     CDS             6431..9154
                     /locus_tag="ACG842_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFQ0248683.1"
                     /translation="MQRLNDYLYQLTLLLKSTPSDNYSARSISQALQLNRSTISSYLN
                     EGVREGLFIKVKSYPVLFLHRTALEELHITLNNSEIESIESLLTISQKPALDQVIGSK
                     GSLKEAIDQIKTAVLYPGKGLPLLLIGASGSGKTFLANKIYEYAVEEKVIHTSAPFID
                     YNCAQYVSNPELLSSALFGYTKGAFTGASQEHTGLLEKADGGVLFLDEVHRLSEEGQE
                     KLFTFMDTGEFSPMGDNSIRKKADVRLVFATTENIYTTFLPTFLRRLPVIVNLPRFQQ
                     RPSFERLSLIDEFFVSESQILAKELSVSDALIHFLMNSDFEGNVGNIKNIIKYACGNA
                     YIHQKSQQTIQVKLLDMPLEYSYKFKEQLNKPRKKRADRIYYPEETRQVQLERNNQLF
                     VQFFDQLLTGFSEVIENNKDIKLFLEEMVTKVTQVMDTLIFQEDYEKEQSLYAILNYH
                     IRQSIDFMRESYGFEQDGNRVVALANYLYVKDNQVIFENSLQEKKTKDQLNEFFANVL
                     ETPYWYAKKILSLLSECLDQPVYEEDIFFITLYFYSLNVTNVKTGIKSIVLAHGYSTA
                     SSMANVANRMLNKNVFQAYDMPLDITLEKIEAKLIQFLGDYRTDSGLILLVDMGSLNQ
                     LGGLLVNHLNGPLLIIDHINTPLLLEVGQQVLNNKSITEIGAEIETSLTFKKQLLLPK
                     KKKKKAIITCCYTGIGSATQIQEILTKCLKDAVRELVIIPYDYKKLLENKNFETPFQL
                     YDVIMIVGTEDPKINQIPYIGLDRLINGGSIGEFVHYLEKELTIEVNDLQKELIFNFS
                     ISKIVENLTILDPDKVLSFVQKAIKELSKLLNFDFSNNQLFLLYLHCSCMIERIVRKE
                     SVDEQEDIEEYMQANGEIMDKIHSAFFEIEREYTIEIPDLELRLLSDIVQG"
     gene            9167..9799
                     /locus_tag="ACG842_002166"
     CDS             9167..9799
                     /locus_tag="ACG842_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFQ0248684.1"
                     /translation="MTMGVIFDMDGVLIDSETFYFERRMNFFQELEIDPGSNQIDDYV
                     GKTEAAIWETLVPEDKSLREELYPKYVDYRKTHPIDFAKALRKEVKPLLDFLKKRDIP
                     LALASSSPAVEIESMLIQCELTEYFSFVISGESLKQSKPHPEIYLKAKQQLDCDFFIA
                     VEDSTIGIKSAKAANIYTAALKQPMQIDQSEADIQIESLQELEQIVLEKG"
     gene            10007..10231
                     /locus_tag="ACG842_002167"
     CDS             10007..10231
                     /locus_tag="ACG842_002167"
                     /inference="COORDINATES: protein motif:HMM:NF015692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HFQ0248685.1"
                     /translation="MQQNQNTNSYQAAILTAVNLGGDTDTTAAVTGALAGILYGGEVI
                     PENWRKSLQNDQLLKTISQRFCDTVLSQRI"
     gene            10333..10566
                     /gene="relB"
                     /locus_tag="ACG842_002168"
     CDS             10333..10566
                     /gene="relB"
                     /locus_tag="ACG842_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB family
                     antitoxin"
                     /protein_id="HFQ0248686.1"
                     /translation="MSQSTITFRIPDDEKELVLEYAKAHNSSLTELYRNAVLDKIEDE
                     IDLRTLRSAIEVSKEKKESGISQEEMEKLLNEL"
     gene            10556..10825
                     /locus_tag="ACG842_002169"
     CDS             10556..10825
                     /locus_tag="ACG842_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFQ0248687.1"
                     /translation="MNYKVIYMPQAQKQLKKMDRNQAKIIVAWIRKNLENTTDPRQHG
                     KGLTGNKSGEWRYRIGNYRILANICDSEIEIQIFSIGHRSTIYKR"
     gene            complement(11004..12305)
                     /locus_tag="ACG842_002170"
     CDS             complement(11004..12305)
                     /locus_tag="ACG842_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HFQ0248688.1"
                     /translation="MPDLHHIKLLLGIKDKNIFITNVESKSIKNIKSLVVSATLSKEI
                     RRCPLCKQMNHEGMIVKNGKKKSLIQLNKCANQLTYLALAKQRYHCRGCHTYFTANTY
                     IVDRNCFIAKQVRYKILEELTEKQAMTTIAKHCGVSWSTVSRTLASLIPMTKVKRNWL
                     PRCLLMDEFRSLKNQVGPYSFSCMDGDTGKLLDILPSRKKKALVSYFMQFERRARLKV
                     KVIVTDMNVSYASLIKECFPKAKLVVDRFHIVKHLIRKFEDIRVRIMKSFDRNDPTQA
                     KYYRQLKALSRLLIKRQDKLVYDKWTKWRNFGWAYLTESEVVDRLLSISDELRIAYFY
                     YQEILQAFHDKEADTFFKLVRTMPNSVPKELHSIKKAFINYESGIRLALELPYSNAKI
                     ENLHTHIKALKRVAYGFRSFRKMKTRIFLLNNLITYESKNI"
BASE COUNT         4030 a         1999 c         2422 g         3888 t
ORIGIN      
        1 ttccttagaa aaattaggtt gtcaaaattt caactcacgt tctgttaaag ggctagagag
       61 tagatttgaa agcatattac tggcttacag tgttctatta agtcgagcac aacgacgttt
      121 tgaaggaact ttgagatatt ctttaggata ttaaaattaa ctagcaccac gggtaaaata
      181 aatagttttt ctatcaagta tattgaacta cgataaattt gttattttgt tttgtttaat
      241 ttcgttgtac ttattggctt tgacttcgat ttctttaaat actgggtaat cggtgtccgg
      301 caaagctagc aaatctgttt tttctgtgtt ccatagatca ttgataatga catgtttttc
      361 gtaatggatt cgctctcgat cgtgtgacag ctccaccgct aatgcctcgt tgaaactggc
      421 gaaatccttc ataatcggtg tagatgaaaa gaagttgtac cgtacatagc cgactttgtt
      481 ttccatacta cctttctcgt ttccgctacg tggattgcac acctgtgtct caaaaccata
      541 gtgcatagaa aatcggagga aaccgtcagt cagcaccgct tgttcagtct ttgtttttgt
      601 ctaagtaacg gccgttgaca tattatcaat ccgtattttc cgaggaacgc cacctgattg
      661 gctgaagagt tctttcatgc cttccaacaa acattcttga ttctcggcgg ggagtacaac
      721 ggcgaatcct gcattactaa aagggaaagt catcactaat accttcacat ctttaaacgc
      781 tccatcgtga caaacttcca tcgtaccaaa atcaagttgc gcttctgctg gcgggtgttc
      841 aagacgctca aagccttggt cttccagtgt ttccaagtag tgtgtcgctt tccaatcttg
      901 aatgaataca cagactgttc ggtaggatcc tggaaagcca tcggctttta aagcagtagt
      961 cattgcttca gcagtcctac gctttttatt tggcagctta gcatcttctg aaagccatag
     1021 ggaaacgatg cctccccact tttctgtata catcatacca gtctttttgc gtgctttagg
     1081 ttgcggcagc aaatcatcat ctgcatattt tttagccgtg cgccaattta catctagctc
     1141 ctttgcaata tgattaatgg atttatcatg attatttcgt aaatttctga tagtattaat
     1201 atcggtcatt gtgagcatac ctttcttacc tccagttaca gtgtcgagac tataacttta
     1261 gaggagtttc aggttgcccg caatggcttt ttatcttaac ttaatcgatt tgcggagttg
     1321 tgtagttttt cgttgcactt gtatgtactt ttattctgcc ataaacagct taatgaatgt
     1381 ccacttgaaa aagtgaataa acgaaataga tgactcgatt taataaggaa gtaaacccac
     1441 acagaaatat aggtgatttt ccttgaatga ccaagatatt gaaagttagt gaaaaaacac
     1501 aaaaaggtag ccctattcac agaaggtctg ccttttgtgt tatcgccagc aaacattaga
     1561 gggggtattt catatagctt ttgtcctctc gacagtccta tcagtttctc ttttaactta
     1621 tctctaagtg ctgtccagtt ttaatagctt ctagcttgtt tcctaactgt gtttttaata
     1681 tgttgaaggc ttttgaacct gtgcagtggc aagtaaggat tttttctact cgtgagtcat
     1741 tcaatttatt tccaattttc attacctctt gctcaggtag acctaaatta tttatataag
     1801 gtatgccaat catatggaag ccaccaatta ggtattttat gggtttagtc ggaaatgttt
     1861 caagagcaat ttccatcata tcgataatgc catgatgact acagccagta aagacaatca
     1921 gaccgtcttc ttgctcaatt acgaccaata attcatctga aaaatcatca taaacaaatt
     1981 tatcatccat ctttttgtat aaattcttgg tggtcgagtt tgctttgcct agggggatga
     2041 tatggaagct cggagcaagt tctgttttcg tttttattgt atgtaaattc tcatgcattt
     2101 taaggatttc atttttagga ataccaacat acagaaacgt ggttaaaatc tttaaataat
     2161 ggggtaaagt tacaccttca gctgtataaa taggggcgtt tcctgatttt aaatatctta
     2221 acccgccggt gtggtcagaa tgaccgtgtg aaataatcac tgcctccaga tcattcaaag
     2281 tttcccccat tttagtcgca ttatagacta attttttatt agcgccagca tcgaagagat
     2341 atttcttccc tgagtagtta attaagagag ataacccatg tccactttta aagttcaaat
     2401 ttattcttga gttttcaaat aaaaccttga tttccactat aacatctcct ttcacatgaa
     2461 cgtgtattca tatattgtag cacattaagt tctatttttt gttgtaatta ccgaaaaaaa
     2521 tattaatgga cgctttttac gctttatttc gtatgtggaa gaaaatttct taggatgtgg
     2581 cgggggattg gggcaaagct aaattccaat tacagtgatc atttaattag acacctttaa
     2641 atacccacac tttcatcaac actataattt agggaaagaa aagtgttggc atgatataga
     2701 aaaaccttta attaacagcg tttcctaaaa ttggcacgca tcttgcttat agaaaagcgt
     2761 aaggatagga ggaatgaaaa tggcaactat aattcttgcc agccatgggg aatttgctca
     2821 aggattaaag caaacagcga caatgattat tggagatagt gtaccaattc atgccttgtc
     2881 agcatttaga gatgaagatg aatctatttt ggtacaaata aaaaaacttt tagccacaat
     2941 agatatagaa gaaacctaca ttttaactga tattctaggt ggtagtgtga ataatgatat
     3001 gctcactatt attaaagagt atgagggatt gcaattaatt actggaatga acttaccttt
     3061 agttatttct attgcaacct ataatggaaa aattggggaa agtgagctag aaaagcttat
     3121 taaagagagt caacaatcag tgattgattg tagaaaatta ttagtagaac aacttactgg
     3181 aggagatgac ctatgattaa gttagtacgt attgaccatc gtttgttgca cggacaggta
     3241 gtattttctt ggagtaaatc attaaatatc aatcgtatta ttattgccaa taaagaggca
     3301 gcgaatgatg aattcaaaaa aatgtctttg aatttatcta aaccgactgg cattaaatta
     3361 acaatttttt cagttgaaca gttactagaa aaaatgccta aaatcgaaac acttaacgaa
     3421 aatatcatgc ttatttttgg taatacaaaa gaaattcgtc aattttgtga agcctatcct
     3481 aaagtacaag aaatcaacta tggagggata atcaaaaaag aaggttccaa gcaatttagt
     3541 aatgccattt tcttgaatga atctgagcaa gaagatgcca aaaaattaaa agctcttggc
     3601 attaaacaat ttattcaaca ggtaccaact tctaaaaaag aagatttgaa tacaatgtta
     3661 taaaggagga atgaaaatgc tcgttcagtc aattggttta gggttaatcg gtgttttttg
     3721 tattttagac tcaaggcttt taggaagaat gaattttgaa cgaccgttga ttgtcagtac
     3781 gcttgttggt ttggtacttg gagacatgga aaaaggatta attgttggag cctctttgga
     3841 attgatgtcc ttaggattag taaatatcgg agctgcttca ccacctgata tgaatatggc
     3901 atcaattatt gctacagcct ttgccatttt atcaaatgcg aatgcagaag cagcattaac
     3961 gattgccatt ccaatttcag tgttaggtca aatactaggt atcttaatgc ggacgatttt
     4021 gtcaaatttg actcacgttg cggatcgctt tatcgaacaa ggaaaattta gccaagcaag
     4081 gagaattcat atactttatg gctcgggatt atatgctctc atgtatttta ttcctatttt
     4141 tctagcaatt tattttggaa cgaatcttgt ttctcagatt gttgatgcga ttcctgagtg
     4201 gctaacaaat ggcttaacgg ttgcaagtaa aattttacca gcatatgggt ttgcgttgtt
     4261 attacaaacg atgttaacga aaaaaatggt gccattttta ttacttggat ttttaattac
     4321 tgcttattct ggattgggaa tcattggtat tgcagccttc gcatgtattg ttgcgtttgt
     4381 catggttcaa gttcaaggta aaaactcaaa tggtaaccaa aatgagccag atccattaga
     4441 tgacttataa ggaggtagat taagtgaaag agaaagatca aaatgcatgg aaatatcggc
     4501 agttcttatg gcgttcatgg gcaattcaag cctcatggaa ctatgaacga caaatgaaca
     4561 tgggttttat gtacggaatt gcaccaattc tagataacat ttacaagaat ccagaagatg
     4621 aagcaatgaa gaaagaagct tacgagcggc atatgatgta ctacaactgt acccctcaaa
     4681 caagtgcatt tgtgttaggg ttaactgctt ctatggagga gcaatactac gaagaccaag
     4741 aaaactttaa tccggaatca atcagtgcag tgaagacttc tctgatggga ccgctatctg
     4801 gtgttggtga ttctttcttt caaggaacga ttcgggtatt agcctttgga ctaggaatta
     4861 atttagccca acaaggtagt attttaggtc caattctagc aatgttaatt tcctttgtac
     4921 cttccttttt agttacttac tatggtggga aaattggcta taacatgggg aataaatact
     4981 tgtctaaact gtatcaagaa ggtttgatgg ataaagtaat gtatgtttgc tccattgtgg
     5041 ggttgatggt cattggttcg atgattgcca gcatgatcgg gattacaaca ccaattcaaa
     5101 tgggtaaagc ttttgtctta caagatgtat tagatagtat tatgccacaa tttttaccat
     5161 taggattaac cttcttcatg tactggttat tgcagaaaaa ggtcaaaaca ggatggatgt
     5221 tggcaatctg tttagtcgga ggaattattt ttagtttgtt aggtatctta caataaggga
     5281 ataaccaata aatttggttg aataatataa ataaggagga attcaattga tgaattcaaa
     5341 agaaattgtt aaagaaatcg tagcgaaaaa agaggtaaaa tcaatcgtat ttgttggctg
     5401 tggcgcttca aaagcggacc tttatcctgc aaagtatttt ctagaccaaa atgcgaagca
     5461 gttacggatt agtcattaca cagccagcga atttaactat gcaacaccag catctgtaga
     5521 tgagtcaact gtcgttattt cagcttcttt aggaggtacg actccagaaa cagttgaagc
     5581 taacgcagta gctaaagaaa aaggtgctac agttattagt ctaacacgaa gcattgactc
     5641 accgttaaca aaagatgcag attatgtaat ttatcatcga tttgcagaga actatgctgc
     5701 taaattagaa aaaacaggct actgtttaga attggcagta gaacttttac atcaagtaga
     5761 aggttatgac cattatgaag caatgcaaga aggatttgct actatctatg atcttgccca
     5821 aaaaagtgca gaatcagctc gtaaagatgc gaagaaattt gctgaacgct ttaaagatga
     5881 aaaagttatt tatgtaatga gtagtggtgc tacacatgaa gtagcatact caacatcaat
     5941 ttgcttgatg atggaaatgc aatggattaa ttctggtacc ttccattcag gagaattttt
     6001 ccacggacca ttcgaaattg tggataaaga cgtaccgttt atattattaa tgaatgatgg
     6061 aaaaactaga aatatagatg ctcgagcatt aactttcctt gaacgctttg atacgaaaac
     6121 agaagttgta gatgccttgg attatggctt gtcttcacat attgctaaag aagtcttaga
     6181 ctactttaac ccattcgtga ttactgcagt atttagagtt tatgcagaag aattgtcaga
     6241 agcaagagaa catccattaa ctaagagacg ttatatgtgg aaactaactt attaaaaaat
     6301 agatcaaact aaataaggtg gcttgagaaa ttaagccacc ttgttttctt caatgtgatt
     6361 ttatacatat ggtaaaataa ctagtacata tggtaaaata actagtacat attgtgaaag
     6421 caggtggcag atgcagcgat taaatgacta tctttatcaa ctaacattgc ttttgaaatc
     6481 tacgccttca gataattatt cagctcgcag tatttcgcaa gctctacagt tgaatagaag
     6541 tacaatcagt agctacttaa atgaaggtgt tcgtgaaggg cttttcataa aagtaaaaag
     6601 ttacccggtt ttatttttac atagaacagc tcttgaagaa ctacatatca cactgaataa
     6661 ctccgaaata gaatcaattg aaagcttgtt aacaatttct caaaaacccg cattagatca
     6721 agtaattggc tctaaaggaa gtttgaaaga agcaattgat caaattaaaa cggcagtctt
     6781 atatcctgga aaagggttgc ctttattatt gatcggcgct agtggatcag ggaaaacctt
     6841 tctagcgaat aaaatatatg aatatgcagt agaagaaaag gtcattcaca catcggcacc
     6901 tttcatagat tataattgtg cacaatatgt gagcaatcca gagttactat cctctgcttt
     6961 atttggctac acgaaaggag catttaccgg agcaagccag gagcatactg gtttgctaga
     7021 aaaagccgat ggaggagtat tgtttttaga tgaagtgcat cgcttatctg aagaagggca
     7081 agagaaatta tttacattta tggatacggg tgaattctct cctatggggg ataatagtat
     7141 tcggaaaaaa gccgatgttc gattagtctt tgcaacaaca gaaaatatat atacgacttt
     7201 cttaccaact tttcttagga gattgccagt tattgttaat ctacctagat ttcaacaacg
     7261 tccgtcgttt gaacgtctgt cattgattga tgaatttttt gtttctgaga gccaaatttt
     7321 agctaaggaa ctatctgttt ctgatgcatt gattcatttc ttaatgaata gtgattttga
     7381 aggtaacgtg gggaatatta aaaatattat caagtatgct tgcggaaacg catatatcca
     7441 ccagaagagt cagcaaacaa ttcaagttaa attattggat atgcctttag agtacagtta
     7501 taaattcaag gagcaactaa ataaacccag aaagaaaaga gcagatcgaa tctactatcc
     7561 agaagaaaca cgacaagtgc aactagaaag aaacaatcag ctttttgttc aattttttga
     7621 ccaattactt actggattct ctgaagtgat tgaaaataac aaggatatta aactattttt
     7681 agaagaaatg gttaccaagg taacacaagt aatggatacg cttatttttc aagaggatta
     7741 cgaaaaagag cagtctttat acgccattct aaactatcat attcgtcaaa gtattgattt
     7801 tatgcgagaa agctacgggt ttgaacaaga tggtaaccgg gtagtcgctt tggccaacta
     7861 tctatatgtc aaggataatc aagtgatttt tgaaaatagt cttcaagaaa agaaaaccaa
     7921 agatcagctt aacgaatttt ttgctaatgt tcttgaaacc ccttattggt atgcaaaaaa
     7981 aatcttaagc cttttatctg agtgtttaga tcaacctgtg tatgaagagg acattttctt
     8041 tattacgcta tatttttata gtttgaatgt cacgaatgtt aaaacaggta ttaagagtat
     8101 tgtgctagct catggttatt ctacagctag tagtatggcg aatgttgcca accgtatgct
     8161 gaataagaac gtttttcaag cttatgatat gcctttagat attacattag agaaaattga
     8221 agcaaaattg attcaattcc ttggagatta tcgaacagat tcaggcttga ttttattggt
     8281 agatatgggt tcactaaacc aactaggcgg tttattagtg aatcatctaa atggaccact
     8341 gttaatcatt gatcatatca atacaccgtt attattggaa gtcggccaac aagtattaaa
     8401 caataaaagt atcaccgaaa taggagcaga aattgaaaca agtcttactt tcaaaaagca
     8461 gttactactt cccaagaaaa agaaaaagaa agccatcatt acttgttgtt atacagggat
     8521 tggtagtgcg acccagattc aggagatttt aacgaaatgc ttgaaagatg cagttagaga
     8581 attagtaatt attccgtatg attacaaaaa gctgttagaa aacaagaatt ttgagactcc
     8641 ttttcaatta tacgatgtca tcatgattgt aggtacagaa gatcccaaaa ttaatcagat
     8701 tccatatatt ggattagatc gcctgattaa tgggggaagc attggcgaat ttgttcatta
     8761 cttggaaaaa gagctgacta ttgaagtgaa cgatttacaa aaagaattaa ttttcaattt
     8821 ttcaattagt aaaattgtcg aaaatttaac cattttggat ccagataagg tgctatcttt
     8881 tgtacaaaaa gcaattaaag aattatcgaa gttattaaat tttgattttt ctaataatca
     8941 gctgtttttg ttgtaccttc actgtagttg tatgattgaa cgaattgtac gtaaagaaag
     9001 tgtggatgag caagaggaca ttgaagaata tatgcaagca aatggagaaa taatggataa
     9061 aattcatagc gctttttttg agattgaaag ggaatacaca attgaaatac cagatttaga
     9121 gttgcgatta ttgagcgata ttgttcaagg ataggagtga attcgaatga caatgggagt
     9181 aatttttgat atggatgggg tactcataga tagcgagaca ttttattttg agagaagaat
     9241 gaacttcttt caagaactgg agatagatcc aggatcaaac cagattgatg attatgtggg
     9301 gaaaacagaa gctgcaatat gggaaacatt agttcctgag gacaaatcat taagagagga
     9361 actttatccg aaatatgtag attatcgaaa aacgcatcca attgatttcg ctaaagcttt
     9421 acgaaaagaa gtgaaaccgt tacttgactt cctcaagaag cgggatatac cattggcact
     9481 ggcatcttct tctccagctg tcgagataga gagtatgctc attcaatgtg aacttactga
     9541 atatttttcc tttgttataa gtggtgagtc attaaaacaa agcaaaccac atcccgaaat
     9601 atatttaaaa gcgaaacagc aattagactg tgacttcttt attgcagtag aggattcaac
     9661 gattggtatt aaatcagcaa aggcagcaaa tatctatacg gcggcactaa aacaaccaat
     9721 gcaaatcgat cagagtgaag cagacataca aattgaaagt ctacaagaac tggaacaaat
     9781 tgttttagaa aaaggttagt gaattaatgg aaataaggct atatatctgt gctagctatt
     9841 ttgactaacc aaaaaaactt gctataattc aggtgtagta aaaggtgctg gtaacacctt
     9901 ttacgaaaat gcttcggcag tgataatggg attggctttt taagtttttt attttgttcg
     9961 caatccctaa ggtcatgtga atgatcctag ggattgctag aatattttgc aacagaacca
    10021 aaatacgaat agttatcaag cagccatatt gactgcggtc aatttaggag gggatactga
    10081 tactactgct gccgtaactg gtgctcttgc gggtattctt tacgggggtg aagtgatccc
    10141 tgaaaattgg agaaaatcgc ttcaaaatga ccagcttctt aaaacgatca gtcagagatt
    10201 ttgcgatacc gtgctatctc aaagaatttg aataatggat atgatgcaaa tcttgtttgt
    10261 actaacgttg tattgacaaa aaggatttta tttagtacaa tgttagtaca aatgatgaac
    10321 ggaggtagaa tcatgtctca atcaaccatt acatttagga ttcccgacga tgaaaaagaa
    10381 ctggtattag aatatgcaaa agcacacaat tcttcattga ccgaacttta tcgaaatgct
    10441 gtactggata aaatcgaaga tgaaatcgat ctacggactt tacggagtgc aattgaagtt
    10501 tccaaagaga aaaaagagag tggtatttct caagaggaaa tggagaaact gctaaatgaa
    10561 ttataaagtc atctacatgc cgcaagccca gaagcaatta aaaaaaatgg acagaaacca
    10621 agctaaaata attgtcgcct ggatcagaaa gaatttagaa aacacgactg acccgcgtca
    10681 gcacggaaaa ggtttgactg gaaataagag tggggaatgg cgctatcgaa ttggaaacta
    10741 tcgaatattg gctaatattt gtgattctga aattgaaatc cagatattct caattggaca
    10801 tagaagtaca atatataaaa gataaccgtt tttggaacga agtagagttt cgaaagtcaa
    10861 tgtataatct tttgaatttt gcataatacc acaggtagaa actacccgat actatcggtt
    10921 ggtttctgct tttttatggc tctttgtcaa ataggactga tagagtgaga taagaacatc
    10981 taaggtagaa ttccttttct actttagatg ttttttgatt cataggtaat cagattattt
    11041 agtaagaaaa ttctggtctt cattttccga aagcttcgaa agccataagc gactcgtttc
    11101 aatgctttga tgtgggtatg cagattttct attttggcat ttgaataagg caattcgagg
    11161 gctaaacgaa tgccggattc atagtttata aaagcttttt ttatagaatg cagttctttt
    11221 ggaacggaat tgggcatcgt tcttacaagt ttaaagaaag tgtccgcctc tttgtcgtga
    11281 aacgcttgaa gaatttcttg atagtaaaaa taggcgattc gtaattcgtc agagatcgaa
    11341 agcagacgat ccaccacttc actttcggtg agatacgccc aaccaaaatt gcgccatttg
    11401 gtccatttgt cataaacaag cttgtcctgt cgtttgatca gaagccgaga caacgccttt
    11461 aattgtcgat aatatttggc ttgtgttgga tcgtttcgat caaagctttt catgattcgt
    11521 acgcgaatat cctcaaattt acgaatgaga tgtttgacaa tgtgaaaacg atccacaacg
    11581 agttttgcct ttggaaaaca ttcctttatc agagaggcat aagaaacatt catgtccgtc
    11641 acgatgacct ttaccttcaa tcgtgcgcgt cgttcaaatt gcataaaata agaaacgaga
    11701 gcctttttct tccgtgaagg aagaatatcc agtaattttc cagtatcgcc atccatacaa
    11761 gaaaaggagt agggaccgac ttgatttttc agtgaacgaa attcatccat taataaacaa
    11821 cgcggtaacc aatttctttt tacttttgtc atggggatca gagaagcgag tgtccgagaa
    11881 acggtcgacc aagaaacgcc gcaatgctta gcaattgtcg tcattgcttg tttttcagtt
    11941 agctcttcta aaattttata gcgcacttgt ttagcgataa agcagtttct atcgacaatg
    12001 taggtattcg ccgtaaaata ggtgtggcag ccacgacagt ggtagcgttg tttcgctaaa
    12061 gcaagataag tcagttggtt cgcacattta ttaagttgaa tgagcgattt tttctttccg
    12121 ttcttaacga tcatgccttc atgattcatc tgtttacaca aaggacagcg acgaatttct
    12181 ttacttaacg tcgcagaaac aacaagactc ttgatattct taatgctctt cgattccaca
    12241 tttgtgatga aaatgttttt atctttgatt ccaagtagta gtttgatatg atgaagatca
    12301 ggcatacaga acgctccttt gattgtgttg tggtgattc
//
LOCUS       DAZKLM010000084        12211 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3764
            SAMEA115706913-rid22582563.denovo.087, whole genome shotgun
            sequence.
ACCESSION   DAZKLM010000084 DAZKLM010000000
VERSION     DAZKLM010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706913
            Sequence Read Archive: ERR13196647
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12211)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12211)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287823.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287823.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,004
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,849
            CDSs (with protein)               :: 2,849
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 41 of 96
            Pseudo Genes (incomplete)         :: 52 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 15 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12211
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706913-rid22582563.denovo.087"
                     /isolate="VRE3764"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(93..650)
                     /gene="frr"
                     /locus_tag="ACG842_002171"
     CDS             complement(93..650)
                     /gene="frr"
                     /locus_tag="ACG842_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HFQ0248689.1"
                     /translation="MADAIMTEAKEKMKKAAQNLQRELGQIRAGRANASLLDRITVNY
                     YGAPTPLNQMASIQIPEARVLMITPFDKSILQDVEKAIMASDIGISPTNDGNVIRLVI
                     PQLTEERRKELAKDVKKEAENAKIAIRNIRRDAIDEYKKQQKNGDITEDDLRGLEKDV
                     QTLTDNSIKEVDKIAADKEKELLEV"
     gene            complement(653..1375)
                     /gene="pyrH"
                     /locus_tag="ACG842_002172"
     CDS             complement(653..1375)
                     /gene="pyrH"
                     /locus_tag="ACG842_002172"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HFQ0248690.1"
                     /translation="MVTPKYQRVVLKLSGEALAGDAGFGIKPPVIKEIIQEIKEVHEL
                     GVEMAIVVGGGNIWRGQIGAQMGMERAQADYMGMLATVMNALALQDTLENVGVPTRVQ
                     TSIEMRQIAEPYIRRKAERHLEKGRIVIFAGGTGNPYFSTDTTAALRAAEIGADVILM
                     AKNNVDGVYSADPKVDANAVKFEELTHLEVIAKGLQVMDSTASSLSMDNDIPLLVFNL
                     NEHGNIRRAILGENIGTTVRGK"
     gene            complement(1511..2392)
                     /gene="tsf"
                     /locus_tag="ACG842_002173"
     CDS             complement(1511..2392)
                     /gene="tsf"
                     /locus_tag="ACG842_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HFQ0248691.1"
                     /translation="MADVTAKMVKELREMTGVGMMDAKKALVEVEGDMEKAVDLLREK
                     GMAKAAKKNDRIAAEGLASVAVKGNTAAIVEVNSETDFVSKNEMFQDLVKEIAELVAE
                     NKPADMEAAMKIKTDKGTIESDLIEATQVIGEKISFRRFEVVEKDDNAAFGGYLHMGG
                     RIAVLTVLDGTTDETVARDVAMHVAAINPRYVNESQIPEAELEHEKTVLTEQALNEGK
                     PANIVEKMVEGRLKKFKAEIALVDQPFVKDPDMTVEKYVASKGATVKTFVRFEVGEGI
                     EKREDNFVEEVMSQVKK"
     gene            complement(2491..3273)
                     /gene="rpsB"
                     /locus_tag="ACG842_002174"
     CDS             complement(2491..3273)
                     /gene="rpsB"
                     /locus_tag="ACG842_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HFQ0248692.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMKKYIFTERNGIYIIDLQ
                     KTVKLVDAAYDYMKSVAEEGGVALFVGTKKQAQEAIKEEATRAGQYFVNHRWLGGTLT
                     NWDTIQKRISRLKQINAMEEDGTFEVLPKKEVAGLNKERERLEKFLGGIADMPRIPDV
                     MYIVDPRKERIAVQEAHKLNIPIVAMVDTNCDPDEIDVVIPSNDDAIRAVKLITSKMA
                     DAFIEGNQGEDQVVEEDFTEENTATSIEEIVDVVEGDNSSAE"
     gene            3632..4111
                     /locus_tag="ACG842_002175"
     CDS             3632..4111
                     /locus_tag="ACG842_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HFQ0248693.1"
                     /translation="MRKAQRHLLIKQVIEEFPIRTQEELLNKLGHYGVTATQATISRD
                     IRDLKIVKAPDENGVSRFVLFREKGTPETRTEEEDRLVQMIEDIVLKVERVHFLTIVN
                     TLPDNAHLFASVLDEIKPPSIVSTIAGFDTIMVISENEEDAKRVEEFLHHPTEVSLF"
     gene            4328..5419
                     /gene="phnW"
                     /locus_tag="ACG842_002176"
     CDS             4328..5419
                     /gene="phnW"
                     /locus_tag="ACG842_002176"
                     /EC_number="2.6.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047304 - 2-aminoethylphosphonate-pyruvate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0019700 - organic phosphonate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-aminoethylphosphonate--pyruvate transaminase"
                     /protein_id="HFQ0248694.1"
                     /translation="MYKLLTSGPLTTSETVKNAMLIDHCTWDQEYKEITQWIRKKLLD
                     LAQVSEGIYTTVLMQGSSSFGVESVLSSVIRPEDTVLICSNGAYRQRMINMCECLKLN
                     YTVYEVSEHQILQAHVITQLIEENPSISALAMVHSETTSGILNNLEEIAAVTQERDLL
                     FIVDAMSSFGGIPICVAELGIDFLISSSNKCIQGVPGFSFVLANKAELEKRKNVSRSL
                     SLDLYDQYRVMENENGKWRFTSPTHTVLAFQQALKELEAEGGIETRYSRYLANNHVLR
                     QKMTEMGFQPFIQEYQGPFITSFLYPKDWRFDFNHFYQYLKQNGFVIYPGKISDHECF
                     RIGNIGEIYPEDILELTAIIKDYKENVND"
     gene            5412..5540
                     /locus_tag="ACG842_002177"
     CDS             5412..5540
                     /locus_tag="ACG842_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HFQ0248695.1"
                     /translation="MIKTVIFDWAGTTVDFGCMAPVHAFRNAFLEKGTQLTDKEIR"
     gene            5544..6089
                     /locus_tag="ACG842_002178"
     CDS             5544..6089
                     /locus_tag="ACG842_002178"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HFQ0248696.1"
                     /translation="MGKLKWDHIQQLLSLPSVKKQWIAIYGQLPQEKEVEELYQQFEH
                     YLFKDLVKHSTLKPDTLETIERLRNAGINIGSTNGFNAPMMTIVAETAKKERYSPDFM
                     ATFEDVEGYGRPFPYMIHKNMQYFKNKSVDEVIKVGDTVSDIQEGKNAGVLTVGVIEG
                     SSLMGISYDEYQKLEMGKKIV"
     gene            complement(6543..8234)
                     /gene="argS"
                     /locus_tag="ACG842_002179"
     CDS             complement(6543..8234)
                     /gene="argS"
                     /locus_tag="ACG842_002179"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HFQ0248697.1"
                     /translation="MNNKDLVAKAVYDAVKDDLTLEQVEQLLENPKSAEHGDVAFPAF
                     SLAKVYRKAPQQIAADLAEKIDSANFEKIEVVGPYLNFFMNKELISKKVLQTVVKEKE
                     HYGDSNIGNQGTVPIDMSSPNIAKPISMGHLRSTVIGNSIGFIMEKIGYQPIRINHLG
                     DWGTQFGKLIVAYKKWGTEEAVKAEPINELLRLYVQFHEVAETEPELNEEARAWFKRL
                     EEGDKEAIQLWQWFRDESMKEFNKIYDLLEVRFDSLNGEAFYNDKMDEIVKLLEEKHL
                     LNEDKGAEIVDLSAYDLNPALIKKSDGATLYITRDLAAALYRKRTYDFKQSLYVVGNE
                     QSYHFKQLKAVLKEMGFDWSDDMHHIPFGLITQGGKKLSTRKGKIVLLEEVLNEAIQS
                     AKEQISEKNPDLENKDAVAKQVGVGAVIFHDLKNDRLNTFDFNLEEVVRFEGETGPYV
                     QYTHARAVSLLEKAGFVPSETADYALNDDTSWEVVKLVQKYPETVLSAGEKYEPSVIA
                     KHAIKLAQAFNKYYAHTKILADDEQKEARLALVYAVTVLLKEDLRLLGLHAPDKM"
     gene            complement(8654..9601)
                     /gene="arcC"
                     /locus_tag="ACG842_002180"
     CDS             complement(8654..9601)
                     /gene="arcC"
                     /locus_tag="ACG842_002180"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HFQ0248698.1"
                     /translation="MAKRKVVVALGGNAILSTDASAKAQQEALMETAKYLVKFIEQGD
                     ELIISHGNGPQVGNLLIQQQAADSEKTPAMPLDTCVAMTEGSIGYWLQNAMGEVLKEK
                     GIDKDVVSLVTQVIVDENDPSFKNPSKPVGPFYTEEEAKEQMNADSTVTFKEDAGRGW
                     RKVVASPKPISIKEARVIETLVDQGVITVSVGGGGIPVVETATGLEGREAVIDKDFAS
                     EKLAEIIDADLLIVLTGVDNVYVNYQKPDQKKLETVTVSEMKQYIDEKQFAPGSMLPK
                     VEAAIAFVEAKPNAKAIITSLENIENLLASEEGTIIVAD"
     gene            complement(9716..10735)
                     /gene="argF"
                     /locus_tag="ACG842_002181"
     CDS             complement(9716..10735)
                     /gene="argF"
                     /locus_tag="ACG842_002181"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003073325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HFQ0248699.1"
                     /translation="MKESVFQGRSLLAEKDFTKEELQYLIDFSEHLKDLKKRGIPHHY
                     LEGKNIALLFEKTSTRTRSAFTTAAIDLGAHPEYLGANDIQLGKKESTEDTAKVLGRM
                     FDGIEFRGFSQKMVEELAEFSGVPVWNGLTDEWHPTQMIADFLTIQENFGTVEGITVA
                     YCGDGRNNMANSLLVTGAILGANMRIVAPKELQPEEEIVKMAEGFAEKSGAQLMITDD
                     VDKGVDGADVLYSDVWVSMGEEDKFEERIKLLKPYQINMEMVEKTHNTDRLIFLHCLP
                     AFHDTNTVYGEQMKERFGITEMEVTDEVFRSKYARQFDQAENRMHSIKAIMAATLGNL
                     FIPRV"
     gene            complement(10826..12055)
                     /gene="arcA"
                     /locus_tag="ACG842_002182"
     CDS             complement(10826..12055)
                     /gene="arcA"
                     /locus_tag="ACG842_002182"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016990 - arginine deiminase activity
                     [Evidence IEA];
                     GO_process: GO:0019546 - arginine deiminase pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HFQ0248700.1"
                     /translation="MDKPIHVFSEIGKLKTVLLKRPGQEVENLTPDIMDRLLFDDIPY
                     LPIAQEEHDNFAKTLQNEGVETLYLEKLAAEAIDAGNVKEQFLNKMLDESHIASNAVR
                     EGLHEFLLSMETQEMVDKIMAGVRTKDIEVRSSSLYDLSADDDYPFYMDPMPNLYFTR
                     DPSASMGNGMTVNKMTFEARRRESMFTEYILKHHPRFANKGIEVWLDRENPDHIEGGD
                     ELILSDKVVAVGISQRTNAKALEKLARHLFAKNSGFEKVLAIKIPNNRAMMHLDTVFT
                     MVDHDKFTIHPAIQSKDGKMDVFTIEPDGDDIKITHSDNLHETMKAALGLDDLVLIPT
                     GNGDEIVAPREQWNDGSNTLAIAPGVVVTYNRNYVSNELLRSYGIKVLEINSSELSRG
                     RGGPRCMSQPLVREDLK"
BASE COUNT         3345 a         2530 c         2203 g         4133 t
ORIGIN      
        1 ataatttttt ctttttttaa tttttaaata aaagaaatat aaataaaaac agaggaagcg
       61 gaatgatccg cccctctgtt tttatgattt tattaaactt ccaataattc tttttcttta
      121 tcagcagcga tcttatctac ttctttgatg ctgttatctg tcaatgtttg tacgtctttt
      181 tctaatccgc gcaaatcatc ttcggtaata tcaccatttt tttgttgttt tttatattca
      241 tcgatcgcat cacgacggat gttacgaatc gcgatttttg cattttctgc ttcttttttc
      301 acatctttag ctaattcttt tctgcgttct tctgtcagtt gcgggataac taagcgaatg
      361 acatttccat cattggttgg actgattccg atatcacttg ccataatagc tttttcaaca
      421 tcttgaagaa tgctcttgtc aaaaggtgtg atcattaaaa cacgtgcttc tggaatttgg
      481 attgatgcca tttggttcaa tggtgttggc gcaccgtagt agttcacagt aatacggtcc
      541 aaaaggctag cgttcgcacg gcctgcgcgg atttgcccaa gttcacgttg aagattttgt
      601 gctgcttttt tcattttttc ttttgcttct gtcataattg catctgccat ttttatttcc
      661 cccttacagt tgttccgata ttttcgccta agattgcacg gcgaatattt ccgtgttcat
      721 ttaaattgaa gacaagcagt ggaatatcat tatccatgct tagtgaactt gcagttgaat
      781 ccatgacttg taatcctttg gcaataactt ccagatgagt cagttcttca aattttactg
      841 catttgcatc tactttcgga tcagcagaat aaacaccgtc tacattgttt ttagccatca
      901 ggataacatc tgcgccgatt tctgccgcgc gtaatgctgc tgttgtgtct gtcgagaagt
      961 acgggttgcc ggtaccgcct gcaaagataa cgatccgtcc tttttctaaa tgacgttctg
     1021 ctttacggcg aatatatggc tctgcgattt gacgcatttc aatcgaagtt tgcacgcgtg
     1081 tcggtacacc cacgttttcc aacgtatcct gtaaagctaa ggcattcata actgtagcta
     1141 acatacccat gtaatcagct tgagcacgtt ccatgcccat ttgtgctccg atttgtccgc
     1201 gccagatatt tccgccgccc acaacgattg ccatttcaac gcccagctcg tgaacttctt
     1261 tgatttcttg gatgatttct ttgattactg gtggtttgat tccaaatcct gcgtccccag
     1321 ctaaagcttc tccactcaat tttaagacta cacgctgata tttcggtgtg accatgttca
     1381 ttcctccaat attttctatt gccattttac catacttaga tgatttcgac actctaagac
     1441 tggtttttct tgttttgctt aaaaaaaggg agcgcacaaa ccgtgcgttc ccgtctgtta
     1501 ttcaacagaa ttattttttc acttggctca taacttcttc tacaaagtta tcttcgcgtt
     1561 tttcgatacc ttcgccaact tcgaaacgta cgaatgtttt aacagttgcg ccttttgatg
     1621 caacgtattt ttcaacagtc atatcaggat ctttaacgaa tggttggtcc accaaagcga
     1681 tttctgcttt gaatttcttc aagcgtcctt ctaccatttt ttcaacgata ttcgctggtt
     1741 tgccttcgtt caatgcttgt tctgtaagaa cagttttttc atgttctaat tcagcttctg
     1801 gaatttgtga ttcgtttaca taacgaggat tgatagctgc tacgtgcata gctacgtctc
     1861 ttgcaactgt ttcgtcagtt gttccatcta gaacagtcaa tacggcaata cgtccgccca
     1921 tatgcaagta tccaccaaat gcagcattgt cgtctttttc tacaacttca aagcgacgga
     1981 agctgatttt ttctccgata acttgagttg cttcgatcaa gtcagattca attgtacctt
     2041 tatctgtttt gattttcatt gcagcttcca tgtctgctgg tttgttttca gcaacaagtt
     2101 ctgcaatttc ttttactaaa tcttggaaca tttcgttttt agaaacaaag tctgtttctg
     2161 agtttacttc aacgattgct gcagtgttac ctttcacagc aactgaagcc aatccttcag
     2221 ctgcaatgcg gtcatttttc ttcgcagcct ttgccattcc tttttcacgt aaaagatcaa
     2281 ctgctttttc catgtcgcct tctacttcaa ctaatgcttt tttcgcatcc atcattccta
     2341 cgccagtcat ttcgcgtagt tcttttacca ttttagctgt aacgtctgcc atgtttaact
     2401 cctcctgtgt gtttttcttt ttaaaaaaag ctgcttcaaa ggagagtgag gcttgtcgcc
     2461 cagcctttga aacagctcca tcattcaaaa ttattctgct gaagaattgt cgccttcaac
     2521 aacatctacg atttcttcga ttgatgttgc tgtgttttct tctgtgaaat cttcttcaac
     2581 tacttgatct tcaccttggt ttccttcgat gaaagcatca gccatttttg aagtaatcaa
     2641 tttaacggca cgaattgcat cgtcgtttga agggattact acgtcgattt catctggatc
     2701 gcagtttgta tcaaccatag ctacgattgg gatatttaat ttgtgtgctt cttgaacagc
     2761 aatacgttct ttacgtgggt caacgatgta cattacatct gggattcttg gcatatcagc
     2821 gataccgcct aagaattttt caagacgttc acgttctttg ttcaaaccag caacttcttt
     2881 tttaggtaga acttcaaaag ttccatcttc ttccattgca ttgatttgtt tcaaacggct
     2941 gatacgtttt tggatagtat cccagtttgt caatgttccg cctaaccaac gatggttaac
     3001 gaagtattgt cctgcacgag ttgcttcttc tttgattgct tcttgtgctt gtttttttgt
     3061 acctacgaat aatgctacgc cgccttcttc agcaacgctc ttcatgtaat cataagctgc
     3121 atctactaat ttaactgttt tttgcaagtc aatgatgtag attccgtttc tttctgtgaa
     3181 gatatatttc ttcatttttg gattccagcg acgagtttgg tgaccaaagt gtacgccggc
     3241 ttctagtaat tgtttcatag aaattactgc catgtttgtt tcctccgatt tggttttatt
     3301 tttcctcttc ttatcttcta ccttgccggc aaactttttc aagcacctac cgacaatcca
     3361 ataagaatgt ggatttgctg atgcataaag caccctcagc tattatacag acaaaagatc
     3421 cgtatcgcaa gagcaaatat acttttctgc tcaacattca atagtttacc tgaaagataa
     3481 aaagaattca agaaaaacag tagatttctc tttgtttctt tattatcctt tttccataaa
     3541 ataattttag gtataaaaga cgaatattat tgggcaatcc ttcttttttt tgttatgata
     3601 catgtgaaat ctttcgtaaa ggagaaagaa aatgcgcaaa gctcaaagac atttgctgat
     3661 taaacaagtc attgaagaat tcccgatccg tacgcaagaa gaactattaa acaaacttgg
     3721 gcattatggc gttaccgcca cgcaggcaac gatttctcga gatatccgtg atctaaaaat
     3781 cgtgaaggca cctgatgaaa atggcgtttc ccgcttcgta ttgtttcgcg aaaaaggaac
     3841 tcctgagact aggactgagg aagaggatcg gctggttcaa atgattgaag acattgtatt
     3901 gaaagtcgaa cgagtccact ttttgacgat cgtcaatacc ttgccagaca atgctcattt
     3961 atttgcatct gttttagatg agatcaaacc tccctctatt gtcagtacaa ttgctggatt
     4021 cgatacgatc atggtcatct cggaaaatga ggaggacgca aaacgagtag aagaatttct
     4081 tcaccatcca actgaagtat cactttttta atcagagcac gtattcctaa tatggaatgc
     4141 gtgttttttt attttcaata ttttccttat tgaataaacc tttacactct ttacaaaaat
     4201 acatcatttt ttattcaaat ttttcaacac cttgattgaa tcacaataat catcatttat
     4261 attggatgtt aacaagagca gcttcttgct ctttgtgtat ttcaaaaaag aaatgaaagg
     4321 aaatcatatg tataaattat taacttctgg accattgacg acttctgaaa cagtgaaaaa
     4381 tgctatgctg atcgatcatt gtacttggga tcaagaatac aaagaaatta cacaatggat
     4441 aagaaaaaaa ttattggatc ttgctcaagt atcggaagga atatacacaa cggtcttaat
     4501 gcaaggtagc agctccttcg gggtagaatc ggtattaagt tctgtgatca ggccagaaga
     4561 tactgtactg atttgttcaa acggcgcata tagacaacga atgatcaaca tgtgcgaatg
     4621 tctgaaactg aactataccg tttatgaggt atcggaacat caaatcctcc aagctcatgt
     4681 gattacacag ctgattgaag agaatccgtc tatctccgca ctagcaatgg tccatagcga
     4741 gactacttcc ggtattttga ataatttgga agagattgct gctgtcacac aagaaagaga
     4801 tcttttgttt atagtggacg cgatgtcaag ctttggtggg ataccgattt gtgtagccga
     4861 actagggatt gattttttga tttccagctc aaacaaatgt attcaaggtg ttccaggctt
     4921 ttcttttgta cttgctaata aagcggaact ggaaaaacga aaaaatgtta gccgaagttt
     4981 gtcacttgat ctatatgacc agtaccgcgt gatggaaaac gagaatggca aatggcgttt
     5041 cacttcccct acccatacgg ttttggcttt tcaacaagcg ctgaaggaac tagaggcaga
     5101 aggaggaatc gaaactcgat attcacgtta tctcgccaat aatcatgtgc tacgacaaaa
     5161 aatgacagag atggggttcc agcctttcat tcaagaatat caggggccat tcatcacctc
     5221 ttttctttac cctaaggact ggcgatttga ttttaatcat ttttatcagt atttaaagca
     5281 aaatggattt gtgatctatc ccgggaaaat cagcgatcat gaatgttttc gtatcggaaa
     5341 tattggcgag atctatccgg aagatatttt ggaactgaca gcaattatca aagactataa
     5401 ggagaacgtt aatgattaaa actgtaattt ttgattgggc aggaacaact gtggactttg
     5461 gctgtatggc tcctgtccac gcttttagaa atgcttttct agaaaaagga acccaactga
     5521 ctgacaaaga aatccgctaa ccgatgggaa aattaaaatg ggatcatatc caacagcttc
     5581 tttccctccc ttctgtcaaa aaacaatgga tagctattta tggacaacta cctcaggaaa
     5641 aagaggtgga ggaattgtat caacaatttg aacattatct gtttaaagat ttggtcaagc
     5701 attctacctt gaaacccgac acattggaaa cgatcgaacg gttgagaaac gctgggatca
     5761 atattggttc cacgaatgga tttaatgctc ctatgatgac aatcgtagca gaaaccgcaa
     5821 aaaaagaaag gtattcacca gacttcatgg caacttttga agacgtcgaa gggtatggtc
     5881 gaccgtttcc ttatatgatc cataaaaaca tgcagtactt caaaaacaag tcggttgatg
     5941 aagtaatcaa agtcggagat acggtctcag atatccagga agggaaaaat gccggagtgt
     6001 tgacagtagg tgtaattgaa ggtagttcgc taatggggat ttcttatgat gagtaccaaa
     6061 aattagagat ggggaaaaag atcgtttaaa acagtctgtt gctcttagat tccaacaagc
     6121 cggcgctgat atttgtgtaa acaatttaaa agagttaaca gaatacattt tgtacgctga
     6181 caagaatcca gaatgacttt tatacaaaat aagaggttgt gaaaaagctg tcctgtatca
     6241 agtaataaaa acagccaagc aaaaatagct cctcatattt ttcgtttggt taggcttatt
     6301 accgcagatg caagcttttg aacaccgttt attcgaaaaa aaaaagggag ctgtgacacg
     6361 acttttgtca cagtcccctt atttgcaaac aaacagtgtt taagagacaa cctctccact
     6421 aaacggtgtt cattcagctg actctcggta tttgctcaaa aaacgataaa atttgaaaag
     6481 ctattttctc ttttgtttct ctaatactcg gatcagaacg ctgatttcac attctctttt
     6541 tcttacatct tatctggtgc atgaagtcct agcaaacgaa ggtcttcttt caataataca
     6601 gtcactgcat aaactagagc taagcgtgct tctttttgtt catcatcagc taaaatcttc
     6661 gtatgagcat agtatttgtt gaatgcttgt gctaatttga ttgcatgttt cgcaatcact
     6721 gacggttcgt atttttcccc agcagacaag accgtttcag ggtatttttg aactaattta
     6781 accacttccc agctggtatc gtcattcaat gcatagtctg ctgtttctga agggacaaat
     6841 cctgcttttt ccaacaggct gactgcacga gcatgtgtat attgcacata aggtccagtt
     6901 tctccttcaa atcgaacaac ttcttccagg ttaaagtcaa atgtattcag tcgatcgttt
     6961 ttcaaatcgt ggaaaatgac tgccccaacc ccaacttgtt tagctacagc atctttgttt
     7021 tccaaatcag ggtttttctc gctgatttgt tctttagcag attggattgc ttcatttaat
     7081 acttcttcta ataagacgat ctttccttta cgagttgata attttttacc accttgcgtg
     7141 atcaaaccaa atggaatatg gtgcatgtcg tcagaccagt caaagcccat ttccttcaag
     7201 acagctttca actgtttaaa atgatagctt tgttcattcc ctactacata tagagattgt
     7261 ttgaagtcgt aagtacgttt acggtacaat gcagcagcta agtcacgtgt aatgtatagt
     7321 gtcgcaccgt ctgacttctt aatcaacgct ggattcaggt cataagcaga taggtcaacg
     7381 atctctgcac ctttatcctc gttcagcaaa tgtttttctt ctaaaagttt gacgatttca
     7441 tccatcttgt cattgtagaa agcttcaccg ttcaacgaat cgaaacgtac ttccagcaga
     7501 tcatagattt tattgaattc tttcatcgat tcatcacgga accattgcca taattgaata
     7561 gcctctttat cgccttcttc cagacgtttg aaccaagcac gagcttcttc atttaattct
     7621 ggttctgttt cagctacttc atggaattgg acgtataaac gaagcaattc attgattggt
     7681 tctgctttaa cagcttcttc tgtaccccat tttttgtaag cgacgatcag ttttccaaac
     7741 tgtgttcccc agtcacctaa atggttgatg cggattggct gataaccgat tttttccata
     7801 atgaaaccga tcgaattacc gataactgtc gaacgcaaat gtcccattga gattggtttg
     7861 gcaatattag gagaagacat gtcgattggt actgttcctt gattcccgat gttgctatcc
     7921 ccataatgtt ctttttcttt cactactgtt tgcaaaactt ttttgctgat aagttcttta
     7981 ttcatgaaaa agttcaagta aggtccgaca acttcgattt tttcaaagtt ggcagaatcg
     8041 attttttctg ctaaatctgc agcgatttgc tgtggcgctt ttcggtaaac ttttgctaat
     8101 gaaaaggctg gaaatgcaac atctccgtgt tcagctgatt ttggattttc tagtaattgc
     8161 tcgacttgtt ccagtgttaa atcatctttg acagcgtcat agacagcttt tgcgacaaga
     8221 tctttattat tcatctttat tcctcctaaa gtgttccctt gattgttaaa caaaaaaaat
     8281 ctctagcgac ggcatactta gtatgccatc actagagacg agatttcccg cggtaccact
     8341 ccaattgtcc taaaaaagga ccagctccat ctgatatcgg tagacgaccg tctgtttaaa
     8401 acagaattct ccaagtgcgc ttcgttgtat cttcttcatt cggcttccac cacttccgaa
     8461 ttcgctagcc aagaatgata caagtactct cttgttcaac aaatcttagt cttattaaac
     8521 tttcttgaat tatatcagaa aaaaaaacga aaggctagtc gtttcataaa aaaaaaatcc
     8581 agatggccta aaggagaaga aataccacct ggatagctat aaatcatttc gtaaatacga
     8641 aagacttaac aaattaatca gctacgataa tcgttccttc ttcagaagct aaaagattct
     8701 cgatattttc taaagaagtg atgattgcct tagcgtttgg ttttgcttca acaaatgcaa
     8761 tagctgcttc tactttcgga agcatgcttc ctggagcgaa ttgcttttca tcaatgtatt
     8821 gtttcatttc tgaaacagta acagtttcta atttcttttg atctggtttt tggtaattga
     8881 catacacatt gtctactcct gtcaatacga tcagcaaatc cgcatcgatg atttcggcta
     8941 atttttctga agcaaaatct ttatcgatta cggcttctcg tccttctaat ccagtagctg
     9001 tttctacaac aggaatccca ccgccaccaa cagagactgt gataactcct tgatcgacca
     9061 gcgtttcgat gacacgtgct tctttaatcg agatgggttt aggggaagcg actacttttc
     9121 tccatccacg tccagcatct tctttgaatg tcacagtgga gtcagcattc atttgttctt
     9181 tggcttcttc ttccgtgtag aatggaccga ctggtttact tggatttttg aatgaaggat
     9241 cattttcatc cacaataact tgagtgacta aagaaaccac atctttatcg atcccttttt
     9301 ccttcagtac ttcgcccatg gcgttttgta accagtaacc gatagatcct tctgtcattg
     9361 caacacatgt atctaaaggc attgccggtg ttttctcaga atcagcagct tgctgctgaa
     9421 tcaataggtt accgacttgc ggaccatttc cgtgggagat gatcaattca tctccttgtt
     9481 cgataaattt cactaaatac tttgctgttt ccattaacgc ttcttgttgg gcttttgcac
     9541 ttgcatccgt agacaagatg gcattgcccc ctaaagcgac aactactttt cgtttggcca
     9601 tttcattcgt cctttcttca gttctaccga attttctaga taacaaaacg agccagcgtt
     9661 ttgtgatttt ccctcaatgc tgactcgttt tttcgtgaga tgaatgtggt tatgcttaaa
     9721 cgcgagggat aaacaagtta cctaatgttg ctgccatgat cgctttgatt gagtgcatac
     9781 gattttctgc ttggtcaaat tgacgagcat atttgctgcg gaagacttca tctgttactt
     9841 ccatttcagt gatgccaaag cgttctttca tttgttcacc gtaaacagtg tttgtatcat
     9901 ggaaagctgg caagcagtgc aagaagatca aacgatctgt gttgtgtgtt ttttctacca
     9961 tttccatatt gatttggtaa ggtttcaaca atttgatacg ttcttcgaat ttatcttctt
    10021 cacccattga tacccataca tcagaataaa ggacgtctgc tccgtctaca cctttatcaa
    10081 catcgtcggt aatcatcaat tgtgcaccgg atttttcagc aaagccttct gccattttga
    10141 cgatttcttc ttcaggttgt aattcttttg gtgcaacgat acgcatattc gctcctaaaa
    10201 ttgcacctgt gactaataaa gagttagcca tattgtttcg accatctccg cagtaagcaa
    10261 cagtgatacc ttctactgtt ccaaagtttt cttggatagt taaaaagtca gcgatcattt
    10321 gtgttggatg ccattcatca gtcaagccat tccaaactgg aacaccagag aattctgcta
    10381 gttcctctac cattttttga ctgaatccac ggaattcgat tccgtcaaac atacgtccta
    10441 gtactttagc tgtatcttct gtggattctt ttttacctaa ttggatatca tttgcaccta
    10501 gatattcagg atgtgcacct agatcgatcg ctgcagtagt aaatgcagaa cgagtacgtg
    10561 tagatgtttt ttcaaataaa agcgcaatat tttttccttc tagatagtga tgtgggatac
    10621 cacgtttttt caagtctttc aagtgttctg aaaaatcgat caaatattgt aattcttctt
    10681 ttgtaaagtc tttttctgct aataagcttc ttccttggaa tactgattct ttcatattaa
    10741 acattctcct ttagattgtt tttatttaag attaaagagg aaatcccgac ggcagctgac
    10801 agtaagactg ccgtcaggaa acaaattact tcaagtcttc acggacaagt ggttggctca
    10861 tgcagcgtgg accaccacga cctcgtgaca attcacttga attgatttct aacactttga
    10921 tgccatagct acgaagcaat tcgttcgata cataatttcg gttataagtg actacaacac
    10981 ctggagcgat tgccaatgtg tttgatccat cattccattg ttctcgaggt gcaacgatct
    11041 catctccatt accagttggg atcagtacaa gatcatctaa tccaagagca gctttcatcg
    11101 tttcgtgtaa gttatctgaa tgagtaatct tgatatcatc accatctggt tcgattgtaa
    11161 agacatccat cttaccgtct ttactttgaa ttgctggatg gatagtgaat ttatcgtgat
    11221 ctaccattgt aaatacggta tccaaatgca tcattgcacg gttgttcgga attttgattg
    11281 ccaacacttt ttcaaatcct gaattttttg cgaataggtg gcgtgctaat ttttctaaag
    11341 ctttcgcatt agttcgttga gaaatcccaa cagctactac tttatcgctt aagatcaatt
    11401 cgtctccgcc ttcgatatga tctggatttt cacgatccaa ccatacttcg attcctttat
    11461 ttgcaaatct tggatgatgt ttcaaaatat attctgtaaa catcgattca cgacgacgtg
    11521 cttcaaaagt catcttattg actgtcatcc catttcccat tgatgcggaa gggtcgcgtg
    11581 taaagtacaa gtttggcatt gggtccatgt aaaatggata gtcatcgtct gctgacagat
    11641 catatagact gctagaacgt acttcgatat cttttgtacg aaccccagcc atgattttat
    11701 ctaccatttc ttgcgtttcc attgatagaa ggaactcatg aagaccctct cttacagcat
    11761 tcgatgcaat atgagattca tccaacattt tattcaagaa ttgttctttg acattgcctg
    11821 cgtcaatcgc ttctgccgct aatttttcca agtaaagagt ctcgacgcct tcattttgca
    11881 atgtcttcgc aaagttgtca tgttcttctt gagcaatcgg aagatatgga atatcatcga
    11941 acaacagacg atccataata tctggtgtca aattttccac ttcttgtcca ggccgtttca
    12001 gtaaaactgt ctttaatttc cctatttcag agaaaacgtg aataggttta tccatgttgt
    12061 gttgcctcct tctttttgat tacatgtata ggatatcctc cttttgtaat cactttcata
    12121 attttgtctc attcaaaatg agacattcct cacattaata ttcgataatt aaataattaa
    12181 acgtttttct atttttaagc gtaatttatt c
//
LOCUS       DAZKLM010000085        11810 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3764
            SAMEA115706913-rid22582563.denovo.088, whole genome shotgun
            sequence.
ACCESSION   DAZKLM010000085 DAZKLM010000000
VERSION     DAZKLM010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706913
            Sequence Read Archive: ERR13196647
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11810)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11810)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287823.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287823.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,004
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,849
            CDSs (with protein)               :: 2,849
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 41 of 96
            Pseudo Genes (incomplete)         :: 52 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 15 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11810
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706913-rid22582563.denovo.088"
                     /isolate="VRE3764"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(173..658)
                     /locus_tag="ACG842_002183"
     CDS             complement(173..658)
                     /locus_tag="ACG842_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HFQ0248701.1"
                     /translation="MSRATEQEAYELLKKLGISFQKVDHPAITSVKNVPFSLPGPQVK
                     NLLLKSRKGKQIYLVILPDEKHADLKQLAEQLKEKRLSFVSEEQLPDLLGVPAGTVTP
                     LALMHDKEKKIQVVIDAAIDRQDTVGFHPNINTTTLIMDFSDFEKILVYLEHLPVYEN
                     L"
     misc_binding    748..1093
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            1138..1779
                     /locus_tag="ACG842_002184"
     CDS             1138..1779
                     /locus_tag="ACG842_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFQ0248702.1"
                     /translation="MEYLIEVMPQLLVGALTTLKLFLFTLIGSLPLGIVLALGLSLHF
                     KPLRYLLQTYVWVMRGTPLLLQLIFIFYGLPTIGIVFDRFEAALIAFILNYAAYFAEI
                     FRGGIQSIPQGQYEAAQVLQLSPFQTVTKIVIPQVTKIVLPSVGNEVINLVKDSSLIY
                     ILGLGDVMRAGKIAMERDATLLPLLGVAIIYLTLTGILTIIMKQVEGKLNYYR"
     gene            1798..2433
                     /locus_tag="ACG842_002185"
     CDS             1798..2433
                     /locus_tag="ACG842_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFQ0248703.1"
                     /translation="MIELKKVNKAFGEKKIIKDLNLIIPDGKTLAIVGPSGGGKTTLL
                     RILAGLESSDSGEFYLDGVPFNPFELKMKEQVVGVVFQDFQLFPHLNIFENITLAPKM
                     VLKQTKEEYTKKAEELAASLGISDLLNIYPYQLSGGQKQRVAIARALAMNPRVLAYDE
                     PTSALDPELRQQVERLILSLKEDGVTQIIVTHDLVFAENVGDQLLKVSPIH"
     gene            2451..3266
                     /locus_tag="ACG842_002186"
     CDS             2451..3266
                     /locus_tag="ACG842_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HFQ0248704.1"
                     /translation="MKKTSFLFALIAGLLLFAGCSNKKTSADNWATINEEKKIVIGLD
                     DTFVPMGFRDKDGKLTGFDIDLAKAVFEQYGITVDFQPIDWSMKEFELNNGTIDLIWN
                     GYSKTPAREKKVQFTKPYMENDQVLITPKESGITNFGQMNNKRLGAQNGSSGYDVFTK
                     QPEVLKEIVKNQDAVLYDSFNEALIDLKSRRVDGLLMDKVYADYYLKQRNELEDFNIT
                     KGSYESEDFAVGARKSDSTLVKKINEALDQLEKTGEFQKISDKWFGEDVTPRN"
     gene            3585..5021
                     /locus_tag="ACG842_002187"
     CDS             3585..5021
                     /locus_tag="ACG842_002187"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFQ0248705.1"
                     /translation="MEHKELTSFPKDFLWGSASAAYQVEGAWQEDGKGESVWDRFVRI
                     PGKTFKGTNGDLAVDHYHRYKEDIALMKEQGLKAYRFSVAWTRIFPNGRGEVNQAGLA
                     FYERLIDELIENDIEPMLTLYHWDLPQALQDEYNGWESRQIIEDFTHYAETLFEAFRG
                     KVHYWISLNEQNIFTSLGYLLAAHPPGVTDPKRMYEVNHIANLANASVINKFHELEMP
                     GKIGPSFAYTPNYPIDSNPENVLAAENAEDLMANYWMDVYMWGKYPIAAMRFLEEKGW
                     APTIEAGDAELLESAKPDFLGINYYQTATNAFNPLNGVGAGKMNTTGKKGSSEETGVP
                     GMYKKVENPFVERTNWDWEIDPEGLRIGLRRITSRYRMPVLITENGLGEYDKLTEDKQ
                     IHDDYRINYLQSHVKAIKEAISDGAEVLGYCTWSYTDLLSWLNGYQKRYGFVYVDQDE
                     TQEGSLERYKKDSYYWYQKLIEENGENL"
     gene            5210..7657
                     /locus_tag="ACG842_002188"
     CDS             5210..7657
                     /locus_tag="ACG842_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HFQ0248706.1"
                     /translation="MAQKKRPTKKRKKTKKTKKQKQQQEQMLTIGVGFALILFAVFGF
                     LKLGFLGILIANGFRIIAGNTYQILCLLLAVLGFWIVIKNTEFSIGKNRRWFGGILFY
                     FGILLLLHAHLFGKLHTGEPNIMGTTWDLLASDIKQSQVDNNVGGGMIGAILYHFTYF
                     LIAQPGSYLAAVLLLAGGAFLFSNLEGYQLLNGIQSIGERVQELLEGDPAKQARKQAA
                     KEERMKQRAEAKEARRLAAKEAAEKEAAEYEKKKAVSQQRENTADEWQEPENTEPEQL
                     SFVPIDSFQENIHPANLEKPVPDTPKQTNTAEGFSDELPEDDGTSLEFEIEAEQENQD
                     YELPTVDLLDSIPTVDQSDEYKKIEKNIGVLEQTFQSFGVDAKVVKASLGPSVTKFEV
                     QPAVGVKVSKIVNLTDDIALALAAKDVRMEAPIPGKSLIGIEVPNGKISMVSFREIIE
                     AQPNHPDKLLEVPLGRDVSGRVQTADLSKMPHLLVAGSTGSGKSVAINGIITSILMRA
                     KPHEVKLMMIDPKMVELNMYNGIPHLLTPVVTNPRKAAQALQKVVQEMEERYEKFAAT
                     GVRNISGYNEFVQQKNLENGTKHPTLPFIVVIVDELADLMMVASNEVEDAIIRLAQMA
                     RAAGIHMILATQRPSVDVITGIIKANVPSRMAFAVSSGTDSRTIIDSNGAEKLLGRGD
                     MLFLPMGENKPIRVQGAFISDHEVERVVQFVTDQQEAHYEEKMMPTDEVETTGAPEQP
                     QDELFEEAKALVVEMQTASISLLQRRFRIGYNRAARLVDELEAHGVVGPSEGSKPRKV
                     FIEQEETSETLPDDNDM"
     gene            7887..8954
                     /gene="rlmN"
                     /locus_tag="ACG842_002189"
     CDS             7887..8954
                     /gene="rlmN"
                     /locus_tag="ACG842_002189"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA];
                     GO_process: GO:0070475 - rRNA base methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HFQ0248707.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            8944..9150
                     /locus_tag="ACG842_002190"
     CDS             8944..9150
                     /locus_tag="ACG842_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0248708.1"
                     /translation="MPNNRILLVFSITIKGAVFLIKELFNLNKRKDSIAKAIKLFLTR
                     KTLIKQRNVLNLMTACLMGVLAII"
     gene            complement(9147..10250)
                     /locus_tag="ACG842_002191"
     CDS             complement(9147..10250)
                     /locus_tag="ACG842_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HFQ0248709.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
     gene            10803..11351
                     /gene="aac(6')"
                     /locus_tag="ACG842_002192"
     CDS             10803..11351
                     /gene="aac(6')"
                     /locus_tag="ACG842_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HFQ0248710.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
     gene            complement(11599..11718)
                     /locus_tag="ACG842_002193"
     CDS             complement(11599..11718)
                     /locus_tag="ACG842_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HFQ0248711.1"
                     /translation="MVFQKPLNNFRYIVYYCHTKIKKNKQKETVTKVLSQSLI"
BASE COUNT         3925 a         1986 c         2534 g         3365 t
ORIGIN      
        1 tcgtagaatc aataaatatg gatttggcta actatggaag aatactcgta ttagttttca
       61 gcgttcatac tagttgttac attgtttgat tggccaaaag aatttctgat atattttgtg
      121 ctaaataaag aaaaagacgc ttgaattggg aaataagaaa actactctat cattacaaat
      181 tttcataaac tggcagatgt tctaagtaaa caagtatctt ttcaaagtcg gaaaaatcca
      241 taatcaacgt agtggtattg atatttggat gaaagcctac ggtatcttga cgatcaatag
      301 cagcatcgat cacgacttgg atttttttct ctttgtcgtg catcaatgct aatggagtca
      361 ctgtacctgc tggaacgcct aggaggtcag gaagctgttc ttcagaaaca aaagataagc
      421 gcttttcttt taattgttca gctagttgtt ttaaatcggc gtgcttttca tcaggcaaga
      481 tgacaagata gatttgtttt ccttttcttg atttcaataa tagatttttt acttgcggac
      541 caggtaggga aaaagggaca tttttgactg acgtaatcgc aggatgatcg actttttgaa
      601 aagagatacc cagttttttt agtagttcat aagcttcttg ttcggttgca cgactcatcg
      661 cgcatcaccc tccttttttt attagtgtat cataaaaaag agttgacaaa tgaaaaataa
      721 tcatgtaaat tacacgtaaa gttaaaacaa atgacatcga aaagaagagt agccgttagg
      781 tgtttttaga gagtctctgg ttggtggaaa gaggcagcag ttaaccgtga aacacatctt
      841 ggagtcgatt ttctgaagcg tatagtagga aaatccgggg agcccgttac agctgcggtc
      901 gttgctagat gaccagataa ggctggcttt tccgtgagga gtcggtaaaa aaaggtggaa
      961 ccacgtgatt aacgtccttt gatcttcgga tcaaaggact ttttgttttc atctgaacgg
     1021 caacgactgt gtgaggtttt ttctgcaagg aaaaaataaa cagaggtgga accacgtata
     1081 atacgtcctc ttggcatttg ccaagaggat ttttttattt taaaggagga aaagaagatg
     1141 gagtatttga ttgaagttat gccgcaatta ttagtaggag cactcacaac attgaaatta
     1201 tttttattta ccttgattgg ctcattacca ttagggattg ttttagcatt aggattgtct
     1261 ttgcatttta agccgctacg ctacttgctt caaacatatg tatgggttat gcggggaaca
     1321 ccgctgttgt tgcaactgat ttttattttt tatgggttgc cgacgattgg gatcgttttt
     1381 gatcgtttcg aagcagcact cattgccttt atcttaaact atgcagctta ttttgcagag
     1441 atctttcgtg gagggataca atcgattcct caaggtcaat atgaagcagc gcaagttttg
     1501 caattaagtc cctttcaaac ggtgacgaaa atcgtcatcc cacaagtgac aaagattgtt
     1561 ttaccttctg tagggaatga agttatcaac ttggtcaaag attcttcctt gatctatata
     1621 ttaggtttag gtgacgtaat gcgtgctggg aaaatcgcga tggaacgcga cgcgacttta
     1681 ctgcctttgc taggcgtggc aatcatctat cttacattga ctggtattct aacaatcata
     1741 atgaaacaag ttgaaggaaa attaaattat taccgctaac tgataggagg aagaaatatg
     1801 attgaattga agaaagtgaa caaagcattt ggtgaaaaaa aaataatcaa agatttgaat
     1861 ctaatcattc cagatgggaa aacattagct atcgtaggtc cttccggtgg cggtaaaacc
     1921 acgcttttac gtatcttggc tggcttagaa agttctgaca gcggagaatt ttatttagac
     1981 ggtgtaccgt tcaacccatt cgagcttaaa atgaaagaac aagttgtagg agtcgtattc
     2041 caagacttcc agctatttcc acatctgaat atttttgaaa acattacctt agcaccaaaa
     2101 atggtattga aacagacaaa agaagaatac accaaaaaag cagaagaatt agcggcttca
     2161 ttagggatta gtgatttatt gaatatctat ccttatcagc tttctggcgg acaaaaacag
     2221 cgggtagcta ttgccagagc attggctatg aatccacggg tattggctta tgatgaacca
     2281 acaagtgctt tagatcctga attacgccag caagtcgagc gtttgatcct atcattgaaa
     2341 gaagatgggg taacccaaat tattgtcacc catgatttag tatttgcaga aaatgtcggt
     2401 gatcaattac taaaagtttc cccaattcat taagaaagga gaagatagag atgaaaaaaa
     2461 ccagttttct attcgctttg atcgcaggtc ttcttctttt tgcaggctgt tcgaacaaaa
     2521 aaacttcagc agataattgg gcgactatca acgaagagaa aaaaatcgtt ataggtttag
     2581 acgatacatt tgttcctatg ggatttagag ataaagacgg gaagctcaca ggatttgata
     2641 ttgatttagc caaagcagtc tttgaacaat atgggattac ggtagacttt cagcccattg
     2701 actggtcaat gaaggaattt gaactgaaca atggaacgat cgatttgata tggaacggct
     2761 attccaaaac gccggcaaga gaaaagaaag tgcaatttac aaaaccttat atggaaaacg
     2821 atcaagtgct gatcacacct aaagaatcag gaatcacaaa ctttggacaa atgaataata
     2881 agagattagg tgcgcaaaat gggtcatctg gttatgatgt atttacgaaa caaccagaag
     2941 tattaaaaga gatagtcaaa aaccaagatg ctgttttgta tgatagtttt aatgaagcat
     3001 tgatcgatct gaaaagcaga cgggtcgatg gtctgctgat ggataaagta tacgcagact
     3061 attatttgaa acaaagaaac gaactagaag acttcaacat aacaaaaggt tcttatgaga
     3121 gtgaggattt tgcagttggt gcacgcaagt cagatagtac attagtgaaa aaaatcaatg
     3181 aagccctaga tcaactagag aaaacaggtg aattccaaaa aatatccgat aaatggtttg
     3241 gcgaagatgt tacgccgaga aattaacaaa taggggactg tgacaaaaga cgtgtcacag
     3301 tccctttttc cgaataaaca gtgttctcct gccaactcct tgtgactcgg agctgaatgg
     3361 caggctcaca acctcttggt aaacggtgtt caaaagccag tttttcggta ttaagccaaa
     3421 tttatgcaaa aatgtagaaa gccattttca caaaattttt cttactactc gaaactaagc
     3481 gactttttca caacctcctt tacataattt tattttgttg tggaaggggt ttcgtgttat
     3541 tctaaaagag gaacgattga aggttatagg aaaggaagag atgaatggaa cacaaagaac
     3601 ttacctcatt tccaaaagat tttctgtggg gctctgcttc tgcagcgtat caagtagaag
     3661 gagcttggca agaagatggg aaaggagaat cagtctggga tcgatttgta cggatacctg
     3721 gaaagacatt taaaggaacg aacggtgatt tggcagtaga ccactatcat cgttataaag
     3781 aagatattgc tttaatgaaa gaacaaggtc tgaaagctta ccgcttctct gtagcttgga
     3841 cacggatttt tcctaatgga cgaggagaag tcaatcaagc tgggctcgct ttttatgaga
     3901 gattgatcga cgaattgatt gaaaacgata ttgaacctat gttgacttta tatcattggg
     3961 atcttccaca agcattgcaa gatgaatata atggttggga atcacgtcag atcattgaag
     4021 atttcactca ttatgcagaa acattatttg aggcattccg cgggaaagtc cattactgga
     4081 ttagtttgaa tgaacaaaat atcttcactt cattaggtta tttgttagca gcacatccgc
     4141 caggtgtgac agacccgaaa cgaatgtatg aagttaacca tattgctaat ttagcaaatg
     4201 catctgtcat caataaattt cacgagttag aaatgcctgg aaaaatcgga ccaagctttg
     4261 cgtatacgcc aaattatcca attgacagta atcctgaaaa tgttctggct gctgaaaatg
     4321 cagaagacct gatggccaat tattggatgg atgtctatat gtggggaaaa tatccgattg
     4381 ctgccatgag gttcttagaa gaaaaaggat gggcaccaac gattgaagct ggcgatgcag
     4441 aattgctaga atctgctaaa ccagattttc ttgggatcaa ttactatcaa acggcgacga
     4501 atgcattcaa tccattaaac ggagttggtg ctggaaaaat gaatacgaca ggaaaaaaag
     4561 gttcatcaga agaaactgga gttcctggaa tgtataaaaa agttgagaat ccatttgtcg
     4621 aacgaacaaa ttgggactgg gagattgacc cagaaggatt aaggatcgga ctgcgtcgta
     4681 tcacaagccg ttaccgcatg ccggtactga ttacggaaaa tggcttagga gagtatgaca
     4741 agttgacaga agataaacaa atccatgacg actatcgaat caattactta caaagccacg
     4801 tcaaagccat caaagaagca atttcggatg gagcagaagt cctaggctat tgcacctggt
     4861 cttatacaga tttgcttagc tggctgaacg gttatcaaaa gcgatatgga tttgtatatg
     4921 ttgatcaaga cgaaacacaa gaaggatctt tggaacgata taagaaagac agttattact
     4981 ggtaccaaaa actgattgaa gaaaatggag agaaccttta gttcacatac ttaaaacaag
     5041 aaggtacggc tggatatggc cgtgcctttt cttgataaaa tcatttagaa attaagaaaa
     5101 actgtaaaat aagctgaaaa atgaaagaaa gcgtcagttc tttcaaacaa aactttagtt
     5161 cccttacttt tttttgtata atgaaacttg atgaaatgag ggataataaa tggcacaaaa
     5221 gaaacggcct actaaaaaaa ggaaaaagac gaaaaaaacg aaaaagcaaa aacagcagca
     5281 ggaacaaatg ctgacgattg gtgttgggtt tgccttgatt ttatttgctg tcttcggctt
     5341 tttaaaatta ggttttttag gaatattgat tgcaaacggc tttcggatca ttgcaggaaa
     5401 tacttaccaa atcctctgtc ttttattagc cgtattagga ttttggattg tcattaagaa
     5461 tacagagttt tcaattggta aaaaccgacg ctggtttggc ggcatactct tttatttcgg
     5521 tatcttatta ttgttacatg cccatttatt tggcaaactc catactgggg aaccaaatat
     5581 catggggacg acatgggatt tgttagccag tgatataaaa caaagtcaag tagacaataa
     5641 cgttggtggt gggatgattg gagcaatcct ttatcatttt acttacttct tgattgcaca
     5701 gcctggaagt tatttagcag cagtgctatt actggcagga ggagcattcc tgttcagtaa
     5761 tctggaaggc tatcagctgt taaatggtat ccaatcaatt ggagaacgag tacaggaatt
     5821 attggaaggt gatcctgcca agcaagctcg aaagcaagcg gcaaaggaag agagaatgaa
     5881 acagcgcgca gaagcgaaag aagcaaggcg cttagctgca aaagaagctg cagaaaaaga
     5941 agctgcagaa tatgagaaga aaaaagcggt gagccagcaa agggaaaata ctgctgatga
     6001 atggcaggaa ccagaaaata cagagccgga gcagctaagt tttgttccaa tcgacagttt
     6061 tcaagaaaat atccatcctg cgaatctaga gaaaccagta ccagatacac caaaacagac
     6121 caatacagca gaaggttttt cagatgagtt gccagaggat gacggtacct cattagaatt
     6181 tgagattgaa gcagaacagg aaaatcaaga ctatgaatta ccgacagttg atttgcttga
     6241 ttctatacca actgtggatc aaagtgatga gtataaaaaa attgaaaaaa atatcggcgt
     6301 attagaacaa actttccaaa gtttcggtgt cgatgccaag gtcgtcaaag ccagcttagg
     6361 accatccgtc acaaaattcg aagtccagcc ggctgtaggg gtaaaagtaa gtaaaattgt
     6421 gaacttaaca gatgatatcg cgcttgcttt agcggcgaaa gatgtccgga tggaagcacc
     6481 gattccaggt aaatcactga ttggtattga agtaccaaat ggaaagatca gtatggtttc
     6541 ctttagagaa atcatcgaag cacaaccaaa ccacccagat aaattactag aagtaccttt
     6601 aggacgtgat gtttctggac gggtacaaac ggctgatctt tcgaagatgc ctcacttact
     6661 cgtggctggt tcgactggta gtgggaagtc agtagcaatt aatgggatta tcacaagtat
     6721 attaatgcgt gctaagcccc atgaagtaaa actgatgatg atcgatccga aaatggttga
     6781 attgaatatg tacaacggca tacctcatct gttgacgccg gtcgtgacga accctagaaa
     6841 agcagcgcaa gccttacaaa aagtagtaca agaaatggaa gaacgatatg aaaaattcgc
     6901 ggcaaccgga gtaagaaata tttctggtta caatgaattt gttcaacaga aaaacttgga
     6961 aaacggaacg aaacatccaa ccttaccatt catcgtagtg atcgtagatg aattagctga
     7021 tttgatgatg gtagccagca atgaagttga agatgcgatt attcgtttag cgcaaatggc
     7081 tcgggcagcg gggattcata tgatcctagc tactcaacgt ccaagtgtgg atgttatcac
     7141 aggaatcatc aaagcaaatg taccttccag aatggcattt gcggtatcaa gcggtacaga
     7201 ttctcggacg atcattgatt ctaatggggc agaaaagctc ttagggcgag gagatatgct
     7261 attcttgcca atgggtgaga ataaaccgat ccgtgtgcaa ggagcattca tttctgacca
     7321 cgaagtagaa cgggtcgttc aatttgttac agatcagcaa gaagctcatt atgaagagaa
     7381 aatgatgccg acagatgaag ttgaaacgac aggtgctcca gaacagccac aagatgagct
     7441 gtttgaagaa gcaaaagcac ttgtcgttga aatgcaaacg gcaagtattt cccttctcca
     7501 aagaagattt cggatcggtt acaaccgagc agcacgttta gtagatgaac tggaagctca
     7561 tggagttgtt ggcccgtcag aaggaagcaa gcctcggaaa gtattcattg agcaggaaga
     7621 aacatcagaa acgttgccgg acgataatga catgtaaaac ttgtgtgatg aagggttgaa
     7681 aaacgaataa ccaataaaaa gacaaggcat gatgatacca aaagaaaaaa tggttttttt
     7741 ccatcttttc taatacggta tgatgtcttg tctttttagt ttttgtgaga ttaaagttct
     7801 attcgattta tttgtgaagt attgcattaa ggaaatgaat catgtatagt atgtagcgaa
     7861 actttgagaa aaagagggaa aatactttgg aaaaacagtc catttatggc ttgacgaatg
     7921 aagaattaat caattggttt atagaaaacg gcgagaaaaa attccgagct gcccaagttt
     7981 gggaatggtt gtatcaaaaa cgtgtaagta atttcacaga aatgacgaat ctatcgaaac
     8041 aattgatcga aaaattatcg gagcatttta tcatcaatcc tctaaaacag atggtcgtcc
     8101 aagaagcttc ggatggtaca gtaaaatatt tgtttgaact ccctgataaa aacatgattg
     8161 agactgtact gatgagacaa gaatatggct tgtcagtttg tgtgacgaca caagtaggat
     8221 gtaatatcgg ctgtacgttt tgtgcaagcg ggctgctcaa aaagaaccgt gatctgactg
     8281 ctggagaaat tgttgcacag atcatgatgg tccaacatta ttttgatgag cgcaatcttg
     8341 gcgaacgcgt ttcacatgtc gttgtaatgg ggatcggtga gccatttgat aattacgaca
     8401 atgtaatgga ctttttgcat atcatcaatg atgccaaagg attggcaatt ggtgctcggc
     8461 acattacagt ttccactagt ggattagcac acaaaatcaa ggaattcgct aataacggct
     8521 tgcaagtcaa tctggctatt tccttgcacg cgccgaataa tgaggtgcga acatcgatca
     8581 tgcggatcaa ccgaagtttc ccaattgaga aattgatgga agctgtggat gagtatttag
     8641 agaaaacaaa ccgtcgaatc acgtttgaat atatcatgtt gaatcaagta aatgatagac
     8701 cagagcatgc gcaacaatta gcagatctat taaaagataa gaaaaaatta gcatatgtca
     8761 acttgattcc atataatcca gtcagtgaac atgatcaata ttctcgcagt ccaaaagctg
     8821 atgtattgag attttatgat gtattgaaga aaaacggcgt aaactgtgtg atacgtaaag
     8881 aacatggtac agatattgat gcagcttgcg gacaattgcg aagcaagcaa atgaaaaaaa
     8941 cagttgccaa ataatcgtat cctactcgtt ttttcaatta ccataaaagg agcagttttc
     9001 ttaattaagg aactgttcaa cttaaataag agaaaagatt ctatagcgaa agctataaag
     9061 ctttttttga caagaaaaac cctcatcaag caaagaaatg tcctcaattt gatgacagcc
     9121 tgcttgatgg gggttttagc aattatttaa ctgattttct tatatccttg ctggctttct
     9181 tgtgtagcat gataagtttc tacgtcaaga atggcctgtt taaatttttc tcgggtgatt
     9241 tcataaggca catgtttaat atcatttgtt gccatggctt tatctaagat tgcttctaaa
     9301 tcggtcgtga cattgagatt catgtcggtt aacgatactg gaagttggtt ctttttcata
     9361 aacacataga ttttatcaaa caaatcgaac tgctgatcca tcatcaataa ggtcagtaca
     9421 ccataactga cagatgctcc gtgaaggtat ccctccattt tttctagaac agtcgtaccg
     9481 taatagaatg agtgtgccat agcagaatta tagttattgt taactaaaac agaaaccaat
     9541 ccggtagtaa caatgatatc cgaaacgact tgttcaattg catatgaggg acgatcgtcg
     9601 gcactatctt tcaatgcttg cgtaccataa gtcaagagac gatcaatgca catttttccg
     9661 atttggattc ctaatctatc cgtgtagcct agatcatcgc cacgagcaga aaaagtagat
     9721 tcgacttctt tggataaagc atcgccaata cctgcccata agtatttatc aggtgcttcg
     9781 gcaataattg ctgtatcaat gaaggtgtgt acaggtggtt ttgaaggaaa gaagagtccg
     9841 tccattacgc cttcttcgtt atagatgaca cataccgcag tgacgggcga acagttcgaa
     9901 ccaatagtgg gaaaggtgaa gactggttta gctagttttt caccaatgac ttttacagta
     9961 tcgcaagcac ggccaccacc gactgcaaac aggacgtctg ctttttggac actttcatgc
    10021 tggatcaact gttcaatatt tccataagtt gaattacccc cgtaccagag actggcgaca
    10081 acctctattt cctgtgtcaa agctactttt attttgtttt ctgcctttga aagtgcagtc
    10141 ttccctccaa tcagcactac ttttgtccca tattctttga cgattccatt tgctttttca
    10201 tacgcatctg ttccaatggt ataatttggt aagaaaacac tttgactcat attcatttcc
    10261 ttctttcttc gttgaaacgg gttaaaattt taaagagtga tcgatctgac tgcggctgta
    10321 gatggagtag aggtgaacct aagacaggat aagtgatgaa taaggtggca tgaacacctt
    10381 gaaatcatca ataagtatcc taaaaacgaa caagtacaaa aacattgaaa acgacaataa
    10441 tgaatttttg ttcctcttag tatttctacc gattcccttt gcttttttgt aattatctaa
    10501 aaattctaaa aattaagatt attatatgaa aacaggtttt atttgtcaat tcataattgg
    10561 ttttttttaa agcaaaagat aatctatatc atgatgaaca aacagagttt tttcctccaa
    10621 ttgatggctt tcctgatctt tcctattttg aataaagtgt ttctgtataa tttttttatt
    10681 ttataagtaa gataaataat gaaagaatag agatactgat tggtagctaa agatttttga
    10741 gcgagaacat ttcgtttttt caaaaggact gttagaataa gcaagaacaa gggggaagac
    10801 gtatgataat cagtgaattt gaccgtaata atccagtatt gaaagatcag ctttctgatt
    10861 tactgagact gacttggccg gaagaatatg gagacagctc ggcagaagaa gtagaagaaa
    10921 tgatgaatcc agaacgaatc gcggtagcag cggtagacca agatgagtta gtaggattta
    10981 ttggtgcaat ccctcaatac ggtatcacag gttgggaatt gcatccatta gttgtagaaa
    11041 gctcccgacg aaagaaccaa ataggtactc gattagtcaa ttacttagaa aaagaagtag
    11101 cttccagagg aggaatcacg atttatttag gtacggatga tttagaccat ggaacaacgt
    11161 taagtcaaac ggacctgtat gagcatacat ttgataaagt ggcttccatc cagaaccttc
    11221 gtgaacatcc gtatgaattc tatgaaaaat taggttataa aatcgtaggt gtcttaccaa
    11281 atgcaaatgg ctgggacaaa ccggatattt ggatggcaaa aacgattatt cctcgaccaa
    11341 attctcaata ataaaaagag gttgtgacag aagtaatcag cttcaagaaa taagaaggaa
    11401 tttacgaaaa ttacttcttg aatttttgtg aaattccaac ttatttccga agaagctact
    11461 tttggaacac cgtgtaccag aggttgtgaa aaagtcgctt agtttcgagt agtaagaaaa
    11521 attttgtgaa aatggctttc tacatttttg cataaatttg gcttaatacc gagaaactgg
    11581 cttttgaaca ccgtttattc agataaggga ctgtgacaag acttttgtca cagtttcttt
    11641 ttgtttattc ttttttatct ttgtatggca ataatagaca atataacgaa aattatttaa
    11701 tggcttttgg aagaccatgg aaaaccttgt acaaagctat tttttctgaa aaattccttg
    11761 aaacctcacg aaaaaatcat tattttatga aaaagacaaa aaaaagtgtt
//
LOCUS       DAZKLM010000086        11805 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3764
            SAMEA115706913-rid22582563.denovo.089, whole genome shotgun
            sequence.
ACCESSION   DAZKLM010000086 DAZKLM010000000
VERSION     DAZKLM010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706913
            Sequence Read Archive: ERR13196647
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11805)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11805)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287823.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287823.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,004
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,849
            CDSs (with protein)               :: 2,849
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 41 of 96
            Pseudo Genes (incomplete)         :: 52 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 15 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11805
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706913-rid22582563.denovo.089"
                     /isolate="VRE3764"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(159..536)
                     /locus_tag="ACG842_002194"
     CDS             complement(159..536)
                     /locus_tag="ACG842_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0248712.1"
                     /translation="MGKLVAVAQSLLDILKWLMEGSNWIFVLPLLLISLFIMFRMVRK
                     KETFLIFIWYAASVGVATLMVELALGSWEACVFALFFAVLYLIIYIPYMKRKSRIGRK
                     GRHTTTFSSTDSLEQSRRTRRRR"
     gene            complement(836..1717)
                     /gene="galU"
                     /locus_tag="ACG842_002195"
     CDS             complement(836..1717)
                     /gene="galU"
                     /locus_tag="ACG842_002195"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006011 - UDP-glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HFQ0248713.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LDSGIEDILIVTGKEKRPIEDHFDANIELENNLREKEKTELLALVEETTQVNLHFIRQ
                     SHPRGLGDAVLQAKAFIGNEPFVVMLGDDLMKDDIPLTQQLMMDYEETQASAVAVMRV
                     PENETSKYGIIDPKTDSEKGRCRVKGFVEKPEMGKAPSNLAIIGRYLLTPKIFEILET
                     QEPGAGNEIQLTDALQTLNQTEAVYAREFKGKRYDVGDKLGYMKTNIEYGLQHSEIGT
                     SLSAYIIQLSKKLRTES"
     gene            complement(1760..2779)
                     /locus_tag="ACG842_002196"
     CDS             complement(1760..2779)
                     /locus_tag="ACG842_002196"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046168 - glycerol-3-phosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HFQ0248714.1"
                     /translation="MKQKIAVLGPGSWGTALAQTLAENGHDVRIWGNVPEQIDEINKY
                     HTNQHFLPDLTIPESIIGYKELAEAVDNADAILFVVPTKAIRSVAQELIGKMNTKPII
                     IHASKGLEQDTHKRISEVLAEEIPEEKRQAIVVLSGPSHAEEVAVHDITTITAASIDQ
                     KAAAYVQKLFMNDYFRIYTNNDVIGVETGAALKNIIAIGAGAIHGLGFGDDAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLIVTCTSVHSRNWRAGNLLGQGHKLEEVLE
                     NMGMVVEGVATTKAAVELAQQLGVEMPITQTIYNVLYNGEDIKKAAKEIMLRDGKMEN
                     EFSLR"
     gene            complement(3412..4416)
                     /locus_tag="ACG842_002197"
     CDS             complement(3412..4416)
                     /locus_tag="ACG842_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFQ0248715.1"
                     /translation="MLKFNEEEQIKAIKGALALRPQVEEIIDKLYAEKFDAVYYLGIG
                     GTYASSMQAVTYMNGKSNLPVFVQHAAEYYTTGNKRLTKDSIVILSSVTGTTQEVVKA
                     VEQIKKVGATLIGFIDKANSKLSQLCDFVVTYPAPGTEQIKFFMAADRLMYLNGEFEA
                     YSEYYEQLERYLPTGLVEAEKKADAFGLSFAEKHRRDSMHYFIGAGNQWGAVYSYAMC
                     YWEEQSWLPSKSIHAAEFLHGTLEIVEETTPVTLFLGEDEQRVLSERVAKLLPKICSN
                     YTLIDTKDYPVEGISEKYRGRVLSFLLMHVVTQRIDAHVEQLNCHPLEIRRYYRQFDY
                     "
     gene            complement(4429..5478)
                     /locus_tag="ACG842_002198"
     CDS             complement(4429..5478)
                     /locus_tag="ACG842_002198"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFQ0248716.1"
                     /translation="MFTMQDYIYEEKEVLSTILKKNDFSTREHMKKTVNLLILATGSS
                     YNACLAAKPALESYGDLTVDIQEPFYFFNYGKLSPSIDTVIAVSQSGKSASTVEAVKM
                     IQKQGLPVVAITNDVQSPLALEAAQIIDLGAGVESVGFVTKGYSATVLQLLLLGIGIG
                     ISKNKISKKIEQDYMQQLRKIINHLPAIIQKTEEFFDGYQSLFRLAQRFVTIGYGPNW
                     GTAKEAETKLTETIRVPSQGFELEAYMHGPYLEADASHLLFFIEGESVNKERSQKLQR
                     YMSRYVGETLTITTKKARNEKTLGLAIECDEYLSVLALVVPFQLFAYKTAVAKGIDLN
                     KKIFEDFDTVLKSKL"
     gene            complement(5495..6316)
                     /locus_tag="ACG842_002199"
     CDS             complement(5495..6316)
                     /locus_tag="ACG842_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFQ0248717.1"
                     /translation="MKNSEIKNSKITKKELNHVFWRSFQMEFSWNYERQMNMAYVYAM
                     IPILKKLYQSKEEMAAALKRHLEFFNTTPHIVTLILGITAAMEEENKDDPEFDVTAID
                     SIKTSLMGPLAGLGDSFFWGTLRLIATGVGTSLAMQGNILGPILFILIFNIPHILFRY
                     LATGWGYRLGTGFLKKIQANGMMESLTLGASIIGLMVVGAMTATMITINIPLKIGSGE
                     NAVTVQSIFDDIVPNILCLGAFGIVFYLLKKEVKPLTILIGLAFFGIFGSWIGIF"
     gene            complement(6303..7082)
                     /locus_tag="ACG842_002200"
     CDS             complement(6303..7082)
                     /locus_tag="ACG842_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFQ0248718.1"
                     /translation="MIVQAVLLGIVAFVAQSEYALGTSLLSRPIVTGLFTGIVLGDVK
                     TGIIMGATLELAFIGSFSVGASIPPDVVTGGILGTAFAITAGAGTETALLLGLPIATL
                     TLILKNIYLGLLIPIMNHKADNYAEEGNYKGVEHMHLLAGIGLSLMLGVVVMVSYMVG
                     SNTIGNLLNMIPDFVQKGLSVATGLIPALGFAMLARLLINKQVAPYFFLGFAIASYLE
                     IPVTGVAIFGAILAVVVVNIMNVRQLPLQTTAGEVEEDEEF"
     gene            complement(7099..7569)
                     /locus_tag="ACG842_002201"
     CDS             complement(7099..7569)
                     /locus_tag="ACG842_002201"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFQ0248719.1"
                     /translation="MIVLTRVDHRLLHGQVAFSWTQTIGADCILIANDDVPTNEIRKT
                     TIKLAKPQGVKLVIKSIDDSIAALKSGVTDKYKLFIVVESIEDAYKLAEAYPEIKTIN
                     LGGIKAKPGTRSIGKAVNILPEEEERLKRLIDKQIEVEIRQVPADKKVDVREVL"
     gene            complement(7581..8000)
                     /locus_tag="ACG842_002202"
     CDS             complement(7581..8000)
                     /locus_tag="ACG842_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFQ0248720.1"
                     /translation="MDREIILASHGKFASGILDSLELIYGKNHSITVFDCYTDENFDL
                     AETVRQLFMRYKDKEIIVLTDLFGGSVNNEFLQYINRDHVYLVAGLNLPLLIELVSRL
                     DAEVETTVLIQQVLTDSKKMIQFCNERLNQKIEEDEF"
     gene            complement(8081..10858)
                     /locus_tag="ACG842_002203"
     CDS             complement(8081..10858)
                     /locus_tag="ACG842_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFQ0248721.1"
                     /translation="MNKLLKKEILCYLENQTAFFDLEHMNEVFTAQRLADHFGVKRNT
                     VSHYLNQLNDEQLLVKINSRPVIYFHKEAFEKQFFKLRTNFYFSIDDLKEEQPFFAQP
                     KDLFSFMIGYDASLKESIEQIKTALYYPDGGLPLLITGESGTGKSYLVNLVYQYCLLH
                     DLLEDSAPFITFNCAQYADNPELLTSNLFGHVKGAYTGAEENKKGAFEAADGGILFLD
                     EVHRLTPEGQEKLFTYLDQGVIYRMGEPNLSRRIKTRLFFATTEEITSHFLTTFIRRI
                     PIRIELPALNQRSRNERLELIYSFFLEEQRKMGSPLSVSGPALSLLTSQKLSGNIGEL
                     KNIVKVSAAKAYAEQREQPEIHVTIHHLQKELLAQPITKNTAQQGIYITQDQTLEQLM
                     EKQQPEQQRIVKSFEQILIDFKKNNCLLYSCEGKLKQEVIQLFDFLLFETSRQEKHEL
                     LVYWTQSIRETFRQMETAYQIKFDGNSVYALSYYLYQRSTIKWLPEEHDIIQLIKELE
                     QQVSQSYPSSYHYVQRILELSKIKLDIEDTSMDRILLTIYLKKAKWSKEKRLPKAMIA
                     AHGYATASSMANVVNRLLQEDLFESFDMPLDVTPQQIASEILDYCENSDVSNGLIILV
                     DMGSLKEIHQFFKKQLSVPLLILNNVTTPLAITVGECLQKNVSLEEIAEETVRQIQPE
                     WRLLYPEENKPKALITTCFTGIGTAVHLSELLEKSLPTACQLKIIPYEYQQLKDKKNS
                     DPLFSIYEVVGMIGTTDPDITNIDYLSLEDLISGEKMSVLAEWLDTTMNASEKEIFNQ
                     RIIRNFSLEKVLDSVTILDTEKVMGEIDHFMRELEVQLNRSISNPKKLALYVHVSCLI
                     ERLIRQMPIETYQGLEKWKQCQKEGLSAIKSAFSVIEEHYSVMIPNSEIAYIYDILSE
                     DTEFLSIEEEF"
     gene            11049..11273
                     /locus_tag="ACG842_002204"
     CDS             11049..11273
                     /locus_tag="ACG842_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0248722.1"
                     /translation="MKSDKYKNRKHTSWKTFREIIGSYLAVVVFLPLLYMAMTEQTRF
                     IGVDTQYIAETGFLFIFILIFAFYKSFHTK"
BASE COUNT         3427 a         2484 c         1910 g         3984 t
ORIGIN      
        1 cataaaaaaa ggaatggctt atcccgatac aacaagcatt cctttaaaaa agattctttt
       61 atataagtga aaaaagcttt tttccaccaa ttaaataaat ctctcatctc aatagtttat
      121 ccattatttt atcttttttt gcgtatacct actaaatatt accgtcttct tctcgttctt
      181 ctactttgtt cgagagaatc tgtactagaa aaagtagttg tatgtctacc ttttctgcct
      241 atccgagact ttcttttcat atatgggata taaatgatta gatacagtac agcaaaaaat
      301 aaagcaaata cgcaagcttc ccaactacct aacgccaatt caaccatcaa ggtagcaacc
      361 ccaacggatg ctgcatacca gataaaaatc agaaacgttt ctttttttct gaccatgcga
      421 aacatgataa atagactaat aagcaatagt ggcaacacaa atatccaatt gcttccttcc
      481 atcagccact tgagtatatc aagcagcgac tgtgcaacag cgactaattt tcccatagtg
      541 aacacacacc tttttttata attaaaaaat tcgccttttt attctactat gaatcttctg
      601 gcttggcttt catttctctt atgtaatcat tttcttttat acaacttaaa aaaaaattaa
      661 accattagca taatattttc tgaatctatt cacgaaagtc tttttctatc tttcgttttc
      721 cacgactcaa ctgatctaga gtcaaaaaac taaaacgaca gttgcgcaaa tgacgcacaa
      781 gctgccgctt taataacaaa tagcctttca ttctcagcag gccacttatt tccagttaac
      841 tttctgttct tagcttttta ctcaactgga taatatatgc acttaatgaa gtcccaattt
      901 ccgaatgttg aagtccatat tcaatatttg tcttcatata tcctaactta tcgcctacgt
      961 cgtatctttt tcctttaaac tctctcgcat agaccgcctc tgtctggtta agagtctgaa
     1021 gtgcatccgt tagctgtatc tcgtttcctg ctcctggttc ttgagtttct aatatctcaa
     1081 atattttagg agtcagtaga taacgtccga taatggctaa attacttggt gcttttccca
     1141 tttcaggttt ttctacaaac ccttttactc tgcaacgacc cttttcgcta tctgttttag
     1201 gatcaataat tccgtattta gatgtttcat tttctggtac acgcatgacg gcaacagcgg
     1261 aagcttgtgt ttcttcataa tccatcatca attgttgagt caatgggata tcatctttca
     1321 tcaaatcatc ccccagcatg acgacaaacg gttcatttcc tataaatgct tttgcttgaa
     1381 gcacggcatc ccccagtcct cttggatgag attgacggat gaagtgaaga ttgacttgag
     1441 tcgtttcttc aactaaagcg agtaattctg ttttttcctt ttcccgcaaa ttgttttcca
     1501 attcgatatt tgcatcaaag tgatcttcaa tgggtctttt ttcttttccc gttacgatca
     1561 agatgtcttc gattcccgaa tccaatgctt cttccacaat aaactggatc gttggtttat
     1621 ccacgattgg aagcatttct tttgccatgg cttttgttgc cggcaaaaaa cgtgttccta
     1681 gcccagcggc aggaatgact gcttttttga ccttcacttg ctgttcctcc taaacgacga
     1741 ttaactattg atcatctatt taacgaagag aaaattcatt ctccattttt ccgtcacgta
     1801 acatgatttc ttttgctgct tttttgatat cttctccatt atacaaaaca ttatagattg
     1861 tttgagtaat tggcatctca acacctagct gttgagctag ctctactgct gcttttgtcg
     1921 ttgccacacc ctcaacgacc attcccatgt tttctagaac ttcttccagc ttatgtccct
     1981 gtcctaacaa gttaccggca cgccaattgc gagaatgaac acttgtacaa gtaacgatca
     2041 agtcccccac accactcaaa ccgataaatg tcagaggatt ggcacccatc gcgacaccaa
     2101 gacggctgat ttctgccaat cctcgagtca tgattgctgc ttttgcatca tctccgaatc
     2161 ctaatccatg gatagcgccg gcaccaattg caatgatatt tttcagtgct gcaccagttt
     2221 ctacgccaat cacatcatta tttgtataaa tcctgaaata atcattcatg aacagttttt
     2281 gtacataggc tgctgctttt tgatcaatac ttgcagctgt aattgttgtg atatcatgta
     2341 ctgccacttc ctcagcatgg ctaggaccag ataatacaac aattgcctgt cttttttctt
     2401 ctggaatttc ttcagccaaa acttctgaga ttcgtttatg cgtatcttgt tctagtcctt
     2461 tacttgcatg tataatgatt ggcttagtat tcattttacc aataagttcc tgtgcgactg
     2521 aacgaatcgc ttttgttgga acaacaaata aaatagcatc tgcgttatcc actgcttctg
     2581 ccaattcttt atatccgata atcgattctg ggatcgtcaa atcaggtaaa aaatgctgat
     2641 ttgtatgata cttattgatt tcatcaatct gttcaggtac atttccccaa atacggacat
     2701 catgcccgtt ttctgccaat gtttgtgcta aagcagtgcc ccaagatcca ggaccaagaa
     2761 ctgcgatttt ttgtttcatg gatattcctc caaataaata attgataatt tccattatag
     2821 tatacatttc ttaaagttgg gactaatttc cattgttttt aagtgtttgt ttacttcttt
     2881 tatcgttttt acttgaaata atcaatgata tgttacaata gtctacattt tatttttaca
     2941 ctcgttatgc tttgcttgtt cctaagagga agcagttttg acaatgttac taccagcatt
     3001 actattagga ggttaggaaa cttctcctag tgagtattga tttaaaatac ataagagaca
     3061 gattgaacat ttctaaatct gtcaggtcct gcaacggaca gattgtctct gttgacggtt
     3121 gacataggaa cttttgtttt agtcaacatt ctggatacaa aacgctatgt aaactggcgc
     3181 aactcgttgt acttggactt ttactacgga tccatgcaac aactaaatag tactattgca
     3241 taacaaaaaa tggttggcac ccacatgtcc cattaaacag attataagtt gaactgtaga
     3301 taatgcaaaa aatgtgtttt ataacgtaat tgatgccaat attttaaaaa aataaaggga
     3361 gcagttatcg ctgctctctt tattttttat attcttttta tccagtctat cctaataatc
     3421 aaactgacga tagtagcggc gtatctctaa tggatggcag ttcaactgct ccacatgagc
     3481 atcgatgcgt tgtgtcacta catgcatcaa taagaaagac aaaacacgtc cgcgatattt
     3541 ttcactgata ccttctactg gatagtcttt tgtgtctatc aatgtataat tgctgcaaat
     3601 ttttggcaaa agtttagcca cacgttcaga taacactctc tgttcatctt cgcctaaaaa
     3661 taatgtgact ggggttgttt cttctacgat ctctaacgtc ccgtgaagaa actcggcagc
     3721 atggatagat ttcgatggaa gccagctttg ttcttcccag taacacatgg catatgaata
     3781 aacagctccc cattgattcc ctgctccaat aaaataatgc atactatcac gtcgatgttt
     3841 ttctgcaaat gataagccaa atgcatctgc ttttttctct gcttccacta accctgttgg
     3901 taaataacgt tcgagctgtt catagtattc tgaataagct tcgaattctc cattcaaata
     3961 catcaatcga tctgctgcca taaaaaattt gatttgttct gttccaggag ctggataagt
     4021 gaccacaaaa tcgcatagct gagacaactt actgttcgct ttatcgataa atccaatcag
     4081 tgtcgctcct actttcttga tttgttctac tgctttcact acttcttgag ttgtaccggt
     4141 aacagaggac aaaataacga tagaatcttt tgttaatctt ttgtttccag tagtgtaata
     4201 ttctgcagca tgctgtacaa agacaggcaa gttgcttttc ccattcatat aagtgacagc
     4261 ttgcatcgag gaagcatacg ttccaccgat tcctagataa tagacagcgt caaatttttc
     4321 cgcataaagt ttatcgatga tttcttctac ttgcggacgt aaagctagtg cgccttttat
     4381 tgctttgatt tgttcttctt cattgaattt cagcatgatt tccacctctt ataatttact
     4441 tttcaagact gtatcaaagt cttcaaaaat ctttttgttc aagtcaatac ctttagctac
     4501 tgcggtttta taagcgaata gttgaaacgg aacgacaagc gctaatacag acagatattc
     4561 atcacattct attgctaatc caagtgtttt ctcgtttctt gctttttttg tggtaatggt
     4621 gagagtctct ccaacgtacc gactcatata tctttgcaac ttttgcgaac gttccttatt
     4681 tacactttcc ccttcaataa agaatagcaa atgcgaagca tctgcctcca ggtatgggcc
     4741 gtgcatataa gcttctaact caaatccttg tgacggaacg cggattgttt cagtcaattt
     4801 cgtctctgct tctttagctg ttccccagtt tggaccatat ccaatggtga cgaaacgctg
     4861 tgctaaacga aataaacttt gatatccatc aaaaaattct tccgtttttt gaataatcgc
     4921 tggtaaatgg ttgattattt tgcgcaactg ttgcatgtag tcttgttcta tttttttgct
     4981 gattttattt ttacttattc caattcctat ccctaagagt agtaactgta aaacagtcgc
     5041 agaatagcct tttgtaacga agcctactga ttctaccccg gcacctaaat caataatttg
     5101 tgcagcttct aaagcaagtg gactttgcac gtcatttgtg atagcaacaa caggtaaccc
     5161 ttgtttttgt atcattttta ctgcttcaac agtagaagca cttttcccgc tttgtgacac
     5221 agcaatcaca gtatcgattg aaggagaaag ttttccataa ttgaaaaaat aaaaaggttc
     5281 ttgaatatcg acagtaagat caccataaga ttccaatgct ggcttcgctg ctaaacaagc
     5341 gttataagat gaacccgttg ctaagatcag taaattcact gtttttttca tatgttctct
     5401 tgtcgaaaaa tcattctttt tcaagatcgt acttagtact tccttttctt catagatata
     5461 atcttgcatc gtaaacattt tctctgctcc ttttttaaaa aatccctatc cacgaaccaa
     5521 aaattccaaa gaaagctagt ccgatcaaga tcgttaatgg tttcacttct ttcttcaata
     5581 gataaaacac tatgccaaat gctccaagac ataagatatt tggaacgata tcatcaaaaa
     5641 tactttgaac agtcacagca ttttctcctg aaccgatttt taaaggaata ttgatggtga
     5701 tcatcgtggc agtcattgct ccaacgacca tcagtccaat aatagacgcg cctaatgtca
     5761 aactttccat cataccgttt gcctggatct ttttcaaaaa acccgttcct aagcgatatc
     5821 cccaacctgt tgctagatag cgaaacagaa tgtgcggaat attgaaaatc aaaatgaaca
     5881 agatagggcc taaaatattt ccttgcatgg ctaaagaggt acccacacct gtggcaatca
     5941 atcgcaaagt tccccaaaag aaagaatctc ctaaccctgc aagaggtccc atcaaagatg
     6001 ttttgatact atcgatcgca gtcacatcaa attcaggatc atccttattt tcttcctcca
     6061 ttgcagccgt gattcctaaa atcagtgtaa cgatatgtgg ggtcgtgtta aaaaactcta
     6121 gatgccgttt caaagctgct gccatttctt ctttcgactg atataacttt ttcaaaatcg
     6181 gaatcattgc ataaacataa gccatattca tctgacgttc ataattccaa gaaaactcca
     6241 tttgaaaaga acgccaaaat acgtgattta gctctttttt cgttattttg ctgtttttga
     6301 tctcagaatt cttcatcttc ttctacctcc cctgcagttg tttgaagtgg tagttgacgg
     6361 acattcataa tattgactac cacaactgct aaaatcgcac caaaaatagc tactccggtc
     6421 acaggaatct ctaagtaaga agctatagca aaacctaaga aaaaatatgg tgcaacttgt
     6481 ttgttgatca gcaaacgagc aagcatcgca aaaccaagag ctggaatcaa ccctgtagct
     6541 actgacaacc ccttttgaac gaagtcagga atcatattta atagatttcc gatcgtattg
     6601 cttcccacca tataagaaac cattaccaca acaccaagca tcaaggaaag accgataccg
     6661 gcaagcaagt gcatatgctc gactccttta taatttcctt cttctgcata attgtctgct
     6721 ttatggttca taattggaat caaaagccct aagtaaatat ttttcaatat caatgtcaac
     6781 gtagcaatcg gcaaacctag taaaagcgcg gtttctgtac cggctccagc tgtaatagca
     6841 aaagccgtcc ctaaaatccc accggtcaca acatcaggtg gaatcgaagc acctactgag
     6901 aacgatccga taaatgctaa ctctaaagtt gcgcccataa tgatcccagt ttttacatca
     6961 cctaacacga ttcctgtaaa cagccccgtc acaatcggac gagaaagcaa cgatgtccct
     7021 agtgcatatt ctgattgtgc gacaaatgca acaattccta ataagactgc ttgtacaatc
     7081 attgaggttc ttcctttctt acaaaacttc tcttacatcc acttttttat ctgctggtac
     7141 ttgacggatt tctacttcta tttgtttgtc aattaatcgt ttcaagcgtt cctcctcttc
     7201 tggtaaaata tttaccgctt ttccgatgct gcgagtacca ggttttgcct tgatccctcc
     7261 taagttgatg gttttgattt ctggatacgc ttctgccagc ttatatgcat cctcgatcga
     7321 ttccactacg atgaataact tatatttatc tgtcaccccg cttttcaacg cggcaatcga
     7381 gtcatcaatt gatttgatca ctagtttgac tccttgtggc ttggctaatt tgatcgttgt
     7441 ctttcgtatc tcattcgtag gtacatcatc attagcaatc aaaatacagt ctgcgcctat
     7501 tgtttgtgtc catgaaaaag ctacttgtcc gtgtaataac cgatggtcga ctcttgtcaa
     7561 tacaatcatt tttcttcctc ctaaaattcg tcttcttcta ttttctgatt gagccgctcg
     7621 ttacaaaact ggatcatttt ttttgaatca gttagcacct gctggatcaa aacagttgtc
     7681 tctacttcag cgtccaaccg agatactagc tcaatcaata gtggcaaatt caatccggca
     7741 acgagataga catgatcccg attgatatat tgtaagaatt cgttgttgac acttccgcca
     7801 aaaagatccg tcagaacaat gatttcttta tctttgtacc tcataaaaag ctgccttact
     7861 gtttccgcta aatcaaaatt ctcatcagta tagcaatcga atacagtaat agaatgattt
     7921 ttcccataga tcagctctaa tgaatctaat atccctgaag caaattttcc atgggaagct
     7981 aagataattt ctctatccac tttttcatct cctcttttca cttacacata ctaaaaaagc
     8041 aagttgcatg ccaaaattag tggcaacaac ttgcttatgt tcaaaattct tcttctatcg
     8101 agagaaactc tgtatcttca ctcaaaatat catagatata ggcaatctca gaattgggga
     8161 tcatgacact ataatgctct tcaatgacac taaacgcaga tttaatagca gataaccctt
     8221 ctttttgaca ctgcttccat ttctctaatc cttgataggt ttcgattggc atttgtcgga
     8281 tcaatcgttc aatgagacag ctcacatgga catacaacgc taattttttg ggattgctga
     8341 tgcttcgatt caactgcacc tctaattctc tcataaaatg atcgatttct cccatcacct
     8401 tctctgtatc tagaatagtc acggaatcca gtactttttc caatgaaaaa ttacgaatga
     8461 tgcgctgatt gaatatttcc ttctctgaag cattcatagt agtgtccaac cattcagcta
     8521 gtacagacat cttttccccc gagatgaggt cttctaatga aagatagtcg atattggtga
     8581 tatcaggatc tgtggtgcca atcattccta cgacttcgta tatggaaaat aatgggtcac
     8641 tgtttttctt atcttttaat tgctgatatt catatgggat gattttcaac tgacaagccg
     8701 taggtaagct tttttctagc agttcactta agtggaccgc cgtaccgatt ccagtgaagc
     8761 aagtagtaat gagtgctttg ggtttgtttt cctctggata gagcagtcgc cattctggct
     8821 ggatctgtcg aacagtctct tctgctatct cttctaatga tacatttttt tgcaggcatt
     8881 cccctactgt gattgctaaa ggtgtcgtca cattattcaa aatcaaaagc ggtacggata
     8941 actgtttttt aaaaaactga tggatttctt tcagagatcc catgtctact aatatgatca
     9001 atccatttga aacatcgctg ttttcgcaat aatcaaggat ttctgaagca atctgctgtg
     9061 gtgtgacatc tagtggcata tcaaatgatt caaatagatc ctcttgtaat aaacgattta
     9121 ctacatttgc catactgcta gcagttgcat aaccgtgagc agcaatcatc gcttttggca
     9181 gtcgtttttc ttttgaccat ttcgcttttt ttagatagat cgtcagtaaa atccgatcca
     9241 tacttgtgtc ttcaatatct aatttgatct tacttaattc cagaattcgt tggacatagt
     9301 gataactaga tggataagac tgagaaactt gttgttctaa ttctttgatt agttggataa
     9361 tatcatgttc ttccggtaac catttgatag tgcttcgctg atagagatag taactcaagg
     9421 catagacact gtttccatcg aatttgattt gataggcagt ttccatttgt cgaaaggtct
     9481 ctcggataga ttgtgtccaa taaaccagca gctcgtgttt ttcttgtcta ctggtctcaa
     9541 acagcaaaaa atcaaataat tgaatcactt cttgctttaa ttttccttcg caagaatata
     9601 gcagacagtt atttttcttg aaatcaatta ggatttgctc aaaactttta acgattcgct
     9661 gctgttcggg ctgttgcttc tccattaatt gctctaaggt ctggtcttgc gtaatataga
     9721 ttccttgctg tgctgtattt ttagtaatag gctgcgcaag tagttctttt tgcaaatgat
     9781 ggatcgtgac atggatttca ggctgttcac gctgttcagc atatgctttt gctgctgaaa
     9841 ctttgacgat atttttcagc tctcctatgt ttccggataa tttctgactt gttaaaagtg
     9901 acaaagctgg tccactgact gacaaagggc tccccatttt tctttgttct tcgagaaaaa
     9961 aagagtaaat cagttctagc cgttcattac gactacgctg attaagtgca ggtagttcga
    10021 ttcggatagg tattcttcga atgaaagttg ttaagaaatg actagtaatc tcttcagtcg
    10081 tagcaaaaaa gagacgtgtt ttgatccggc gtgacaaatt aggttctccc atacgataaa
    10141 tcacaccttg atcaagatag gtgaacagtt tttcctgacc ttctggtgtt aagcgatgta
    10201 cctcgtctaa aaacaagatc ccaccatctg ctgcttcaaa agcccctttt ttattttctt
    10261 ctgcaccagt gtacgctccc ttaacatgtc caaacaaatt gctagtcaaa agttcaggat
    10321 tatctgcata ttgtgcacaa ttaaaagtga tgaatggtgc agaatcttct agcagatcat
    10381 gaagcagaca atactgatag accaaattaa ctaaataact tttacctgtg ccactttcgc
    10441 ctgtaatcaa aagtggtaat ccgccatctg gatagtaaag agctgttttg atttgttcga
    10501 tactttcttt caaactggcg tcgtatccga tcataaaaga gaataagtct tttggttgcg
    10561 cgaaaaaagg ctgttcctct ttgagatcat cgatagaaaa ataaaaattc gtgcgtaact
    10621 taaaaaattg tttttcaaaa gcttctttgt ggaaataaat cactggtcga gaattgattt
    10681 tgactagtag ttgctcatca tttaactggt tcaaataatg actgactgtg tttcttttta
    10741 ccccaaaatg atcagctaac ctttgtgctg taaagacctc gttcatatgt tctaaatcga
    10801 aaaaggctgt ctggttctct aagtaacata aaatttcctt cttcaacaac ttattcaccc
    10861 ctcctccatc atagaaaacg gattcatttt gtcaataaaa tctttaataa agaattgaaa
    10921 aatatatctt attatagaca gcagcctact tatcccttat aatttttatt ctaaaaaacg
    10981 tacagttcct tccagttttt atactggaat ttcaacatat gaaagaataa ggaggctatg
    11041 actattcaat gaaatcggat aaatataaaa acaggaaaca tacttcctgg aaaacttttc
    11101 gagaaatcat cggatcttat ctcgctgtag ttgtattttt gcctttgctt tatatggcta
    11161 tgacagaaca aactcgtttt attggcgtag atacgcaata tatagccgaa acaggatttt
    11221 tattcatctt tatcttgatt tttgcgttct ataaatcctt ccatacaaaa taagattcac
    11281 agatttcaac tttcctgtct gtttttagct attacaaatc cttatatgtg gtataacaac
    11341 aatttattgg cataatgagg aaaaacgagt tatgatgctt tcagagagta aagaggtact
    11401 gttgtcacca tcctttttat tgattacgta caccactctt agttttttcc cattgattac
    11461 gaaaacaagg tagaaacatt gatgttccta ccttgttttc tttttttatt ttcagatcaa
    11521 aatctcaagt agcttttgac ttgatgtcgg gaggttgtct tcgttccatc aaacctgata
    11581 gaatctgcgc caaaccatag ttttctccac tattatttat tcctctaaga tagtacccac
    11641 aataaacgaa taaatatttt tctcattttc atactccttc tttgaaaatc aattagattt
    11701 ctctcaaccc acttttttac aaaaattacg tagcaaaaaa gattgcaaga acaaattaaa
    11761 aaagaagtaa atagagtttc atctataaac atttacttga tatat
//
LOCUS       DAZKLM010000087        11744 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3764
            SAMEA115706913-rid22582563.denovo.090, whole genome shotgun
            sequence.
ACCESSION   DAZKLM010000087 DAZKLM010000000
VERSION     DAZKLM010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706913
            Sequence Read Archive: ERR13196647
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11744)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11744)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287823.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287823.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,004
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,849
            CDSs (with protein)               :: 2,849
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 41 of 96
            Pseudo Genes (incomplete)         :: 52 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 15 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11744
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706913-rid22582563.denovo.090"
                     /isolate="VRE3764"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(192..806)
                     /locus_tag="ACG842_002205"
     CDS             complement(192..806)
                     /locus_tag="ACG842_002205"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HFQ0248723.1"
                     /translation="MFERSPSELTERENYKLLIGSVIPRPVAVVTTKSAKDIVNIAPF
                     SYFNIVSSNRPILSLAIQRKQDAVKDTAKNILETREAVIHILDEDNVEEVNKTAANLP
                     NEESELYLTDLTLTDSVIISVPALTEAKVRFETSLYEHVEIRNKNKVTADLFLLEVQK
                     YHLNETVYDDKTGRIDPRKLNAVSRLAGNDYAAIGEIFTIARPE"
     gene            complement(953..1636)
                     /locus_tag="ACG842_002206"
     CDS             complement(953..1636)
                     /locus_tag="ACG842_002206"
                     /EC_number="5.1.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009385 - N-acylmannosamine-6-phosphate
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006051 - N-acetylmannosamine metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019262 - N-acetylneuraminate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HFQ0248724.1"
                     /translation="MDTLEQIKGGVVVSCQALENEPLHSSFIMGRMALAAKEGGAVGI
                     RANTTEDINEIKRIVDLPVIGIIKRDYDGSEVFITATEKEVEELLETQAEIIALDATS
                     RKRPTEKTTAELVQMIHEHGRLAMADISTFEEALQAQEDGFDLLSTTLAGYTEYSRKT
                     QTPDFDLLNQIIEQVDIPVIMEGHTDSPEQVEKAYELGAFSVVVGSIITRPQLITKRY
                     VEAASKANN"
     gene            complement(1745..2203)
                     /locus_tag="ACG842_002207"
     CDS             complement(1745..2203)
                     /locus_tag="ACG842_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HFQ0248725.1"
                     /translation="MYQQVEEYVKKFQEKYPSYQSEKLGFFSFGDTEKMADELSELVV
                     KKIKTGTSSGYDLYKQENEPLPKVGQLDVVLNGKNEPVCIIKNINVTILPFSEVTKEH
                     AWKEGEGDRTLAYWRKVHAEFFKHAYKEALNIEFTEEKLVVYEEFEVIFF"
     gene            complement(2287..4050)
                     /locus_tag="ACG842_002208"
     CDS             complement(2287..4050)
                     /locus_tag="ACG842_002208"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HFQ0248726.1"
                     /translation="MSTVLHVLDAINSPADVKKLSVEEMKQLAEEMRQKILQKDSDVG
                     GHVGPNLGVVELTIAFHYVFDSPKDKIVWDVSHQSYGHKLLTGRKNGFEKGHYREVSG
                     YSNQHESKHDYFTVGHTSTSISLATGLAKARDLKKESGNVVAFIGDGSLSGGLAFEGL
                     NNGGVLQSNLIVLVNDNEMSIDENHGSLYKNLAELRQTNGKSSNNLFKAMGFDYRYVD
                     NGNDLETVIRLFEEVKDIDHPIVLHVHTEKGRGYQPAIDNKMKYHWHVPFDLSTGEAK
                     QVTSGETFNMIIMDYLDKKMQTSTAPIAAVNAAIPGAFGLKTFQEKHPDHYFDVGIAE
                     QHSITFASALASQGVRPVVFQSSTFLQRAYDQLSHDLAINENPVIILVGGGTISASDR
                     THQGTFDIPYLSSIPNIKYLAPATKEDMISMLDWAFEQTAQPVAIRIPTHGVEHGTAI
                     ANDYSIPAYQTVNNGGKVAILALGGFLELGKETVKAFRKKGIEATLINPGTITENDVE
                     TLDKLKETHELVITLEDGSLSGGFGENISRYYGDSSMNVLNFGAEKEFTDSVPVGQLY
                     ERYHLTPKQIVEDSLRVLNIQ"
     gene            complement(4271..5731)
                     /locus_tag="ACG842_002209"
     CDS             complement(4271..5731)
                     /locus_tag="ACG842_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0248727.1"
                     /translation="MLENYIERNIFRKVYLCEQLFEFQEIDIEQTAISLRVTTPTILH
                     DLESLAECLEYCIKEQVREKHKYKLVFKHGIALSELTQFLYGQSYFLKFLSYYFNGIF
                     TSTELADLEFISLSKVYTIKKIVLDFFKENDYLKNKEIIIPEFDSRNILLALVRYVNW
                     EGYENKNPYVEEACNELIDYVETHFFKRRYSEDEKSFIIRGIEIAIGRKAFPLGLKEE
                     DKKSAETKPLFQLVCQGLEEQKGILDLQEEDVYYIFSLFNSRNYTNENMELLRKDFEV
                     VYKNFVLNDLSLNELILQIISIAEIDDAENFIFKQSFLQFVRTLWADGQVFLPERIYL
                     LSEKEQLLYGKIVEILEEWKKKNQIQLRWNENFIRKFVKSLSLINSEQAGRQEIEIFV
                     VSESSVKQFFYRTQFHLYAEEGIAEINPILFRSLEELPDECLCAKKRVVLCDAASYQN
                     GLETEKTKIYPISLKGANIHLYHIAQELHLLKKHIS"
     gene            complement(5939..6865)
                     /gene="glsA"
                     /locus_tag="ACG842_002210"
     CDS             complement(5939..6865)
                     /gene="glsA"
                     /locus_tag="ACG842_002210"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HFQ0248728.1"
                     /translation="MEVKELEQLIGKNFHYYQTGQVADYIPALAEVDPKQLGMAIYDL
                     DTKQLIEGGDADVRFAIESMSKVPVLLLAIKDNGIEKVFQTINTEPTGFAFNSIMNME
                     INHRKHPMNPFVNAGAIATTSLIAGKDADEKFERILSFMKEICDDPNITLNEEIYHSE
                     SRTGDINRSLAYYMKGNQMIEGDVPEILDAYFRQCSLNVTATGIAKLAAVLANKGIAP
                     WNGKQIITEESATIVKSIMTTAGLYDESGEFSVHVGVPAKSGVGGGLMAAVPNRYGIG
                     VFSPALDPFGNSAAGIQLLKDVVKELDADIFE"
     gene            complement(6888..8315)
                     /locus_tag="ACG842_002211"
     CDS             complement(6888..8315)
                     /locus_tag="ACG842_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFQ0248729.1"
                     /translation="MQQSKTKIPAKKLTFFGFLAMTISMVVSLYEYPTFATSGFSLVF
                     FLLLGGLLWFIPVALCAAEMATVKGWEKGGVYTWVSRTLGKRFGFAAIFFQWFEITVG
                     YLTMLYFLTGALSYATGIGAIQNNKYLKLAILLIIFWAILLSQLKGTKYTSLIARIGF
                     VAGILLPALVLFGLGIHYVASGAPLQTILSMKTLIPDFSKLPTLVVFVSFILAYMGVE
                     TSASHANEMENPKKDYPLAMFVLVILAIILDTFGGLTVATTVPQHGLSLNTGVIQSLE
                     FLLRHIDPNLAWIAKILAILVCLGVIGEIASWVTSPSKALHVAAEEGLLPEYFAKENT
                     HGVPAHLMIANGIVATIWAAVFTLSGGGNNLSFLAAMSLTVVIYLMLYFMFFAGYLKL
                     VFKDDSLERAYQVPGGKVVKVIVACLGLLTSLFAFCISFVPPASIAAGQHGIYETILI
                     VSYLIVLVIPFTIYACRHKFQHAEK"
     gene            complement(8346..9560)
                     /locus_tag="ACG842_002212"
     CDS             complement(8346..9560)
                     /locus_tag="ACG842_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium channel activity
                     [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HFQ0248730.1"
                     /translation="MNTLPINTGDTAFMILCTAMVCLMTPGLAFFYGGLARKNNILTI
                     MSQSFLSVGITTIMWVFGGFGLAFGRDIGGVIGNPADFFLMRHVTSFPNMFHGATIPF
                     LMFFLYQLMFIVITVPLMTGAFAGRLNLKGYIILVIFWNLLIYFPVCHWIWGGGFLDQ
                     MGFRDFAGGAVIHTTAGFGSLACILTLGQRKIPLGERSQHSSLMAAAIGTGLLWFGWF
                     GFNSGGALRADEQAVNAFGSTFIALAFAMITWLIIAKVKGNGFDFVDVLTGSVAGLAA
                     ITPCAGYVEAKSAMLIGIIAGIVCHAAVDFRKKKQWDDALDVWGVHGMGGFTGTILIG
                     IFASNSHLLTNPSSWYFLGIQVIGVIITAVYAYVLTTVILKSAMHFTTITTTKEEQEE
                     GLDFFFFGNKLN"
     gene            complement(9646..9873)
                     /locus_tag="ACG842_002213"
     CDS             complement(9646..9873)
                     /locus_tag="ACG842_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0248731.1"
                     /translation="MKNKFENRSDTRCGSFLYILYEVFFIPYEKISLKIKKDENKRFS
                     IKRRPFIFCLYLCEKRKRAQHIKLLLIVCLL"
     gene            complement(9901..10143)
                     /locus_tag="ACG842_002214"
     CDS             complement(9901..10143)
                     /locus_tag="ACG842_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFQ0248732.1"
                     /translation="MGFIWALIVGGIIGAIAGAITNKGSSMGIIANIIAGLVGSAIGQ
                     ALLGTWGPTIAGMAIIPSLIGAIILVLVVSFFARKL"
     gene            complement(10175..10732)
                     /locus_tag="ACG842_002215"
     CDS             complement(10175..10732)
                     /locus_tag="ACG842_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HFQ0248733.1"
                     /translation="MENKNMNTTNGSVLANEKGENRMMNGELTYEDKVIQKIIGYALK
                     DVDGLLTLDGGFFSNMAEKLVNTDNVTAGINTEVGKKQVAVDLDVIVEYGKDIEDIYN
                     QIKELISTEVNNMTHLDVIEVNVNVADIKTQEEYQKDQETVQDKVTEAAKSTGQFASR
                     QTDKAKSAVGKGAQKVKENNEPRVQ"
     gene            complement(10745..10933)
                     /locus_tag="ACG842_002216"
     CDS             complement(10745..10933)
                     /locus_tag="ACG842_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HFQ0248734.1"
                     /translation="MKELFSAYKLPIIFGGLGLLLAVLWLTIGFFKTLLIIIMTAIGA
                     GIGFYLKQSGIVDQFFKS"
     gene            complement(10946..11512)
                     /gene="amaP"
                     /locus_tag="ACG842_002217"
     CDS             complement(10946..11512)
                     /gene="amaP"
                     /locus_tag="ACG842_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HFQ0248735.1"
                     /translation="MNKGLKIIGVIMSLLLLSVFLAIALINAPYIMPEQLERFRFFTL
                     TNYYMQQYIFWAAVVFAVLAIIILLVVLFYPKSRGTFVMKREDGKLTIDKKAIEGLVR
                     SHLHEEEFIHSPKVRIRSTKNRIHIHVNGDLKRTSSLVGKTGVLMQDIEEEVTKVLGT
                     KETVKVAVTYSGYEEQEDTRDYKHSRVE"
BASE COUNT         3439 a         2439 c         1927 g         3939 t
ORIGIN      
        1 atcgtttgat ctggcatttc tatttcctta ccatctaaag taacagtagt aacgacaaaa
       61 tggtcttgca atggcggaac ttctaagtac tgcccttgca gaaaatcatt cactgtttca
      121 tattcttttt tcatttcttt tgaatctgtc gatggtttat agcttatttt taacataact
      181 agtgctcctt tttattcggg acgtgcaatc gtgaaaattt ctcctattgc tgcataatca
      241 tttcctgcca aacggcttac agcattcaat tttcttggat cgatccttcc tgtcttatca
      301 tcatagactg tttcatttaa atgatatttc tgtacttcta gcaaaaataa atcagctgtt
      361 actttatttt tattccgtat ctcgacatgt tcatataagg atgtttcaaa acgaactttt
      421 gcttctgtca atgctggtac tgaaatgatt actgaatcag ttaaagtcaa gtcagtcagg
      481 tacagttcgc tttcctcatt aggcagattg gctgctgttt tatttacttc ttctacgttg
      541 tcctcatcaa ggatatggat aacagcttct cttgtctcaa gaatattctt tgctgtgtct
      601 tttactgcat cttgttttct ctggatcgcc aaggataaga tgggtcgatt cgaactgacg
      661 atattgaagt aactgaaggg tgcgatattc actatatctt ttgctgattt agtcgttact
      721 actgctactg gtctagggat gactgaacca atcagcaact tgtaattttc acgttctgtc
      781 aattctgaag gactccgttc aaacatggtt tcgccttctt tctataaaat tcgttaaaaa
      841 gcagatcttg aagacaaaat gaaaagtaat gcttttttca tcttcttaga atctgcttta
      901 aactatctat caatttttct tttttgtcaa gaatctgtgc cgtgcgtgtt ttttagttat
      961 ttgctttact tgcggcttcg acatatcttt tagtgatcaa ttgcggacgg gtgataatag
     1021 atccaactac gactgaaaaa gcacctaatt catatgcttt ctctacttgt tcaggagagt
     1081 cagtatgtcc ttccataatt actgggatat cgacttgttc tatgatttga ttcaataaat
     1141 caaaatcagg tgtctgcgtc tttcgtgaat attctgtata gcctgctaaa gtcgtagaca
     1201 aaagatcaaa accatcttct tgcgcttgaa gcgcttcctc aaaagtcgaa atatctgcca
     1261 tagctaaccg accgtgttcg tgaatcattt ggacaagttc agcagttgtt ttttctgtcg
     1321 gacgtttacg agaagttgca tccagtgcaa tgatttctgc ttgcgtctcc aaaagttctt
     1381 ccacttcttt ttctgtcgca gtaataaaca cttctgaccc atcgtaatct cttttgataa
     1441 tccctattac cggtaaatca acgattcgct tgatttcatt gatatcttct gtcgtatttg
     1501 ctcgaatacc aactgctccg ccttcttttg cagccaaagc cattctcccc ataataaaag
     1561 aactgtgtag cggctcgttt tccaaagctt gacaagaaac gacgactccg cctttgattt
     1621 gttccagtgt atccacaata tctcctccag taatgttata taaaaataca gcttcatata
     1681 aaaatgtaac ccttttctat tcaggagaca ataatgagct atttctttca gcagaccttt
     1741 cttactaaaa gaaaattact tcaaattctt cgtatacgac cagcttctct tctgtaaatt
     1801 ctatgttcaa cgcttcttta taagcatgtt taaaaaactc agcatggact ttccgccaat
     1861 aagctaatgt ccgatcccct tctccttctt tccaagcatg ctctttcgtg acttcactaa
     1921 aaggcaagat ggtcacattt atatttttga tgatacaaac tggttcattt tttccattca
     1981 ggaccacgtc taattgacca acttttggta aaggctcatt ctcttgttta tacagatcgt
     2041 aaccacttga tgtaccagtc tttatctttt ttacaactag ttcgctcaac tcgtctgcca
     2101 ttttttctgt atccccaaat gaaaagaaac ctaatttttc tgattgataa ctaggatatt
     2161 tttcttggaa ctttttgaca tattcttcta cttgctgata cataacatcc acccctttgc
     2221 tttttataat gaaaaaacca tccagatact agtataaaca attatctgga tggtgagcat
     2281 aatgaattat tgaatattca agacacgcag actatcttca acgatttgtt ttggtgttaa
     2341 atgataacgt tcataaagct gccctacagg aacgctatcc gtgaattctt tctctgcacc
     2401 aaaatttaat acgttcattg aagaatcacc ataataacga ctgatatttt cgccaaatcc
     2461 tcctgacaaa ctaccatctt ctaatgtgat gactaattcg tgtgtttctt ttaatttatc
     2521 taatgtttct acgtcatttt ctgtgatcgt tcctggattg atcaacgttg cttcgattcc
     2581 tttttttcta aaagctttta ctgtttcttt tcctaattca agaaaaccgc ctaaagctaa
     2641 aattgctact tttcctccat tatttactgt ttgataggct ggaatactat aatcatttgc
     2701 gatagctgta ccatgttcta caccatgtgt aggaattcgg attgctacag gctgcgctgt
     2761 ttgttcgaac gcccaatcta gcatagagat catatcctct tttgttgctg gagcaagata
     2821 tttgatgttc gggatacttg ataagtaagg aatgtcaaat gttccttggt gtgttcggtc
     2881 actagctgag atcgttccgc caccgactaa aataatcact ggattctcgt tgatcgctaa
     2941 atcatgtgat aattgatcat atgcccgttg caagaaagta cttgattgga agacgactgg
     3001 acgtactccc tgagaagcca aagcagaagc gaaagtgata gagtgctgtt cagcgatccc
     3061 cacgtcaaaa taatgatctg gatgtttttc ttggaatgtt ttcagtccaa aggcgcctgg
     3121 tatcgctgca ttgactgcgg caataggagc tgtacttgtt tgcattttct tatccaaata
     3181 atccatgatg atcatattaa atgtttctcc agaagttact tgctttgcct ccccggtgga
     3241 cagatcaaat ggtacatgcc aatgatattt cattttatta tcgattgccg gctgataacc
     3301 gcgacctttt tccgtatgga cgtgaagtac aatcggatga tcaatatctt ttacttcttc
     3361 aaataaacgg atcaccgttt ccagatcgtt tccattatcc acgtaacgat aatcaaatcc
     3421 cattgcttta aataggttat tgctgctttt accgttggtt tgacgaagct cagctaagtt
     3481 tttatataaa ctgccatgat tctcatcaat agacatctca ttatcgttta ctaatacgat
     3541 tagattggat tgtaaaacac caccattgtt caatccttca aaagctaagc caccacttag
     3601 ggagccgtca ccgataaatg ctaccacatt tcctgattct ttcttcagat ctctcgcttt
     3661 agctaaacct gttgctaagc tgatagatgt agatgtgtgc cccactgtaa aatagtcatg
     3721 tttactttca tgttggttgg aatatccgct cacttcacgg taatgtcctt tttcaaaacc
     3781 attttttcga ccagttaaaa gcttatgacc ataagactga tgggaaacat cccaaacgat
     3841 tttatccttt ggtgaatcaa atacataatg aaacgcaatc gttaattcta ctacacctag
     3901 attcggacca acgtgcccac ctacatcgct gtccttttgc aagatttttt ggcgcatctc
     3961 ttctgccaac tgtttcattt cctcaacaga taattttttg acatctgcgg ggctattgat
     4021 tgcatccaat acatgtaata ctgtactcat aatacattct ctcctccgta ctatcttctt
     4081 ttaatgaaaa tagatttgtt acactttatc tattctacta cttagagttg actctaagtc
     4141 aatagcattt taaaaaaatt tcagagaaag cttgttatat aagtagatct tcgcacgaat
     4201 caggactcaa ataaaaacct tctacagaca attaaaaagc tttgtagaag gtctcctaat
     4261 taaggtctca tcaagaaata tgttttttta ataggtgaag ttcctgagca atatgataca
     4321 aatggatgtt tgcacctttt aatgagattg gatagatctt tgtcttttca gtttctaatc
     4381 cattttgata gctagcagca tcgcacaaaa ccactctttt tttcgcacat agacattcat
     4441 caggcaattc ttctaatgaa cgaaataaaa taggatttat ttcagcgatg ccttcttccg
     4501 catacaaatg aaattgggtt cgataaaaaa attgtttgac tgaactttct gaaacaacaa
     4561 aaatttctat ttcctgtctc cccgcttgtt cactgttgat caaagaaaga cttttgacga
     4621 attttcgaat gaaattttca ttccatctta gttggatttg atttttcttt ttccactctt
     4681 ccaatatttc tacaattttt ccgtacagaa gttgttcttt ttctgataga agatagatcc
     4741 tttccggtaa aaatacttgt ccatctgccc acaacgtacg gacaaattgt aaaaaggatt
     4801 gcttgaaaat aaaattttct gcatcgtcga tttcagcgat actaataatc tgtaaaatca
     4861 attcattcag tgataggtca ttgagaacaa aatttttata aacaacttcg aaatcttttc
     4921 ttaacagctc catgttctca ttggtataat ttctactgtt gaacaaagaa aaaatataat
     4981 agacatcttc ttcttgtaag tctagaattc ctttttgctc ttccaatcct tgacagacta
     5041 attgaaataa cggcttcgtt tctgcgcttt tcttatcttc ttctttcagt ccaaggggaa
     5101 atgcctttct tccgattgcg atctctattc ctcgtatgat aaaggacttt tcatcttccg
     5161 aatatcttct tttaaaaaaa tgagtctcta cataatcaat caactcgtta caagcttctt
     5221 ctacatacgg atttttattt tcatagcctt cccaattgac ataacgtact aaagctagca
     5281 agatgttccg cgagtcaaat tctgggataa tgatttcttt atttttcaaa taatcatttt
     5341 ctttaaaaaa atccaatacg atttttttga ttgtatatac tttgctcaaa gaaataaatt
     5401 caagatcagc cagttctgta ctcgtgaata taccattaaa ataataactt aaaaacttta
     5461 aaaaataaga ttgaccgtac agaaactgtg tgagctcaga aagagcaata ccatgtttga
     5521 atacgagttt atacttatgc ttctctctta cttgttcttt tatacaatat tctagacact
     5581 cggctagact ttctagatcg tgaaggatcg ttggtgtagt cacccttaga gaaatagctg
     5641 tttgttcgat gtctatttct tggaattcaa ataattgttc acacagatac acttttctaa
     5701 atatatttcg ctcgatgtaa ttttccagca ttcctgatac acgcctccag tttttcttat
     5761 gattttattt aatcatatct gtaaatcgta tttttgtttc ctttttattt agaaaagccc
     5821 tttcaaaaac caataaaaaa agaaactggg acaaaagtca aacaagctca caatttcagc
     5881 ctcattgtag gtagctttga gccgtttgga cttttacccc agcctctttc aagaatggtt
     5941 attcaaagat gtcagcgtct agttctttga ctacatcttt caacaattgg ataccagctg
     6001 cgctgttacc aaatggatct agtgccgggc tgaagacacc tatgccgtaa cgatttggca
     6061 cagctgccat caacccgccg cctacaccgc tttttgcagg tacaccaaca tggacggaaa
     6121 actctcctga ttcatcatac aaaccagcag ttgtcataat tgatttgacg attgtagcac
     6181 tttcttcagt aatgatttgt ttgccattcc aaggagcaat tcctttattt gccagcacag
     6241 cagctaattt tgctatgcct gtagcggtga cattgaggga gcattgtctg aaataagcat
     6301 cgagtatctc cggtacgtct ccttcgatca tttgattacc tttcatatag taagcaagag
     6361 agcgattgat atcacctgtt cttgattcag aatgatagat ttcttcattc aatgtgatat
     6421 taggatcatc acagatctct ttcataaatg acaagattcg ctcaaatttt tcatctgcat
     6481 ctttaccggc aatcaacgag gttgttgcaa tcgctcctgc attaacaaaa ggattcattg
     6541 gatgtttgcg atgattgatt tccatattca tgatagaatt gaaggcaaaa ccggttggtt
     6601 ccgtgttgat tgtttgaaag actttttcta ttccgttgtc tttgattgct aataacaata
     6661 cgggaacttt tgacatgctt tcgatggcaa agcgcacatc ggcgtctcct ccctcaatca
     6721 gttgttttgt atccaaatca taaatcgcca tacctaattg tttgggatcc acttcagcta
     6781 atgctggaat gtaatccgcc acttgtcctg tttgataata gtggaaattt tttccaatca
     6841 attgttctaa ttcttttact tccatttgaa taaccagctt tcatttttta tttttctgca
     6901 tgctggaatt tatgacggca tgcgtagatc gtaaatggaa taactaagac gataagataa
     6961 gaaacaatca gaatcgtttc ataaatccca tgctgtccag ctgcgatact agcaggtgga
     7021 acaaatgaga tacaaaaagc aaaaagtgaa gtcaaaagac ctaaacaagc aacgattact
     7081 tttacaactt ttccgcctgg aacctgatag gctctttcaa gactgtcatc tttaaaaacc
     7141 agcttcaaat agcccgcgaa aaacataaag tacaacatca ggtagattac aacagtcaat
     7201 gacatcgctg caagaaatga aagattattc cccccgccgc ttaatgtaaa gacggctgcc
     7261 cagattgttg caacgatccc atttgcgatc atcaaatgag caggaacacc gtgtgtgttt
     7321 tcttttgcaa aatattcagg aagtaatcct tcttctgctg ctacgtgaag tgcttttgaa
     7381 ggagaggtta cccaagaagc aatttctccg atgacaccta aacaaacaag aatcgcaaga
     7441 attttcgcaa tccatgcaag atttggatca atgtgtctta ataggaattc taacgattga
     7501 atcacccctg tattaagaga caaaccatgt tgaggtactg tagtagcgac ggtcaatccg
     7561 ccaaaagtat ctaaaatgat tgccagaata accaatacaa acattgctaa tggataatcc
     7621 tttttagggt tttccatttc atttgcatga ctggcagatg tttctacgcc catatatgct
     7681 aaaataaagg aaacaaaaac aaccagcgta ggcaatttcg aaaaatcagg aatcaatgtt
     7741 ttcatagaca gtatcgtttg tagtggagcg cctgaagcga cataatggat ccctaagcca
     7801 aacaagacta atgctggcaa caagatacca gctacaaaac cgatccgagc aatcaatgaa
     7861 gtgtatttgg ttccttttaa ctgtgagagt aaaatagccc agaaaataat caataggatt
     7921 gccaatttca gatatttatt gttttgtata gcaccaatac ccgttgcata ggataatgca
     7981 ccagttaaga aatacagcat ggtcaagtag ccaactgtta tctcaaacca ttggaaaaag
     8041 attgcggcaa aaccgaatcg ttttcccaat gtgcgactga cccaagtata gacaccgcct
     8101 ttttcccatc ctttcaccgt tgccatctct gctgcgcaca atgccacagg gataaaccat
     8161 agcaaaccgc ctaataacaa gaaaaatact aagctgaagc ctgaggtcgc gaatgtggga
     8221 tattcatata atgaaacgac cattgagatg gtcatagcca aaaagccaaa gaacgttaat
     8281 ttttttgcag gaattttcgt tttgctttgt tgcatgaatc ttcctctttt ctatttgaat
     8341 ttgtttcaat tgagtttgtt gccaaagaag aaaaagtcca atccttcttc ttgttcttct
     8401 tttgtagtag tgatcgttgt aaagtgcatc gccgatttta aaatcactgt tgtcagcaca
     8461 taggcataca cggcagtaat gatgacacca atcacttgaa tccctaagaa gtaccagctg
     8521 cttggattcg ttagtaaatg gctgttagat gcaaagatcc ctatcagaat cgtacctgtg
     8581 aatccgccca tgccatgaac accccacaca tcaagtgcat catcccattg tttcttttta
     8641 cgaaaatcaa cagcagcatg acagacgata cccgcaatga tcccaatcag catggcactt
     8701 tttgcttcta cataaccggc acaaggcgta atggctgcca atccagcaac ggatcctgtt
     8761 agtacgtcga caaaatcaaa gccatttcct tttacttttg caatgatcag ccaagtaatc
     8821 atcgcaaatg caagagcgat gaatgttgaa ccaaatgcat tgactgcttg ttcatctgct
     8881 cgaagtgcac caccggaatt aaaaccgaac cagccaaacc aaagcagtcc tgtaccgata
     8941 gcagctgcca taagactgct atgctgactt ctttctccta aaggaatctt tctttgacca
     9001 agtgtgagga tacatgctaa agagccgaat ccagctgtcg tgtggatgac tgctccacca
     9061 gcgaaatctc taaatcccat ctgatccaaa aatccgccac cccatatcca gtggcaaaca
     9121 gggaaataaa tcagcagatt ccaaaaaata acaagtataa tatagccttt taaattcaat
     9181 ctccctgcaa aagctcctgt catcaaagga acggtaatca cgataaacat cagttggtaa
     9241 agaaagaaca ttaaaaatgg aatcgttgca ccatgaaaca tgttggggaa gcttgtaaca
     9301 tgtctcatca aaaagaaatc tgccgggttt ccaataacac cgccgatatc ccgtccaaag
     9361 gccaatccga atccaccaaa tacccacata atcgttgtta tcccaacgga gagaaaagat
     9421 tgagacatga tcgttagtat attattcttc cgtgctaatc cgccgtaaaa aaaagctaga
     9481 ccaggagtca tcaggcaaac cattgctgta cataaaatca tgaatgctgt atctcctgta
     9541 ttgataggta atgtattcat ttttttcgcc tcctttctac gattacgagt ataaaagaag
     9601 gtgtagttgt ttttttcttc tgtttttttg aattaatact tgcatttata aaagacagac
     9661 aattaataaa agcttgatgt gttgtgctct ttttcgtttt tcacacaaat aaagacaaaa
     9721 aataaacggt cttcttttta tagaaaaccg tttattttcg tcttttttta tttttaaaga
     9781 tattttttca taaggaataa agaatacttc atataaaata tataaaaaag aaccgcagcg
     9841 agtgtcgcta cggttctcaa atttgttctt catttcaaca taagtttttc ttcaatttgc
     9901 ttataattta cgtgcaaaga atgaaacgac taatactaaa atgattgcac cgatcaatga
     9961 tggaatgata gccattcctg caattgttgg tccccaagtt cctagtaaag cttgtccaat
    10021 tgctgaacct actaaaccag caatgatgtt tgcgatgatc cccattgatg atcctttatt
    10081 cgtgattgct cctgcaattg ctccaatgat accgccgacg attaatgccc aaataaatcc
    10141 cattttaaat tcctcctaac tgacttctta cttcttattg tacacgtggt tcgttatttt
    10201 ctttgacttt ttgtgcacct ttgcctacag cagattttgc tttatctgtt tggcgtgaag
    10261 caaattgtcc tgtggatttt gctgcttctg ttactttgtc ttgtactgtt tcttgatctt
    10321 tttggtattc ttcttgtgtt ttgatgtctg caacattcac attcacttcg ataacatcta
    10381 aatgtgtcat attgtttact tcagtactga tcaattcttt gatttggttg taaatatctt
    10441 cgatgtcttt accgtattct acgatcacat ctagatccac agctacttgt ttcttaccta
    10501 cttctgtatt gatacctgct gttacgttat ctgtgttgac taatttttca gccatatttg
    10561 agaaaaatcc gccatctaat gtcaatagac catctacatc tttcaatgca taaccgatga
    10621 ttttttggat gactttgtct tcataagtca attcaccatt catcatgcgg ttttctcctt
    10681 tttcatttgc caaaactgat ccatttgttg tattcatgtt tttgttttcc atctttgttt
    10741 cctcctatga tttaaaaaat tgatcaacta ttcctgactg tttcaaataa aaaccgattc
    10801 ctgcgccaat tgctgtcatg attatgatca acaatgtctt gaaaaatcca atcgttaacc
    10861 ataaaacagc tagcagtagt ccaagacccc caaagataat agggagcttg tatgctgaaa
    10921 atagttcttt cattatttgg ccctcctatt ccactcgaga gtgcttgtag tctcttgtat
    10981 cttcttgttc ttcataaccg ctgtatgtga ctgccacttt gaccgtttct tttgttccga
    11041 gtactttagt tacttcttct tcgatatctt gcatcaatac acctgttttc cctactaatg
    11101 aagaagttcg tttcaagtct ccgttgacat ggatatggat tcgatttttc gttgaacgta
    11161 tccgtacttt aggtgaatga atgaactctt cttcgtgtaa atgggaacgt accaaccctt
    11221 cgatcgcttt tttgtcgatt gttagtttgc catcttcccg tttcataacg aaagttcctc
    11281 ttgactttgg atagaaaaga acgactagca agatgataat agcaagaacg gcaaatacta
    11341 cagctgccca gaaaatatat tgttgcatgt agtaattcgt taaagtaaag aagcgaaatc
    11401 gttccaattg ttcaggcata atatacggcg cattgatcaa cgcaatcgct agaaatactg
    11461 aaagtagcag taaactcatg ataacaccga ttatttttaa ccccttattc attttccacc
    11521 tcctataaac ttatttttac cctgtaaata actctaccga caatcacagt atagatagcg
    11581 aacagttagc atagcatcag taaataaagc gctaccaaac attgctggat cataacggcg
    11641 ttttattgcc agtctgatac tgaaataaca attgcttact tattctctat ttacttttga
    11701 ttgctggcta tacttaaagg atattattca agattaaaaa ttgc
//
LOCUS       DAZKLM010000088        11255 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3764
            SAMEA115706913-rid22582563.denovo.091, whole genome shotgun
            sequence.
ACCESSION   DAZKLM010000088 DAZKLM010000000
VERSION     DAZKLM010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706913
            Sequence Read Archive: ERR13196647
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11255)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11255)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287823.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287823.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,004
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,849
            CDSs (with protein)               :: 2,849
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 41 of 96
            Pseudo Genes (incomplete)         :: 52 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 15 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11255
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706913-rid22582563.denovo.091"
                     /isolate="VRE3764"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..480
                     /locus_tag="ACG842_002218"
     CDS             <1..480
                     /locus_tag="ACG842_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0248736.1"
                     /translation="YSSVVNMVKEMNFQGTYSNEQMENKLEEFENFDYVPIGYKKDQL
                     LLKNHQVTEDGQVIYSSFSPNKDEITFTFKSSKGKTIVLPVFRYKGLTVTVNSKNATV
                     LKQGGPFVTVKSESGINKVTIRYSYTFFSKISLFISIITLIVLLFYCIKLKIRTSHE"
     gene            complement(747..1916)
                     /locus_tag="ACG842_002219"
     CDS             complement(747..1916)
                     /locus_tag="ACG842_002219"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HFQ0248737.1"
                     /translation="MKKGKHAKKRKYSFLMIVVVLFSLSAFSTGTILGWQFYNNEHSQ
                     IVAHAQSAQKKLILTTNEQQLVEKIDQLLRKNDYIGSIYVKKNNRIIMEKGYGYANKL
                     TQQPNDPSLYYQIGSVQKALTTLLILKQVERGHLSLDTKLAQFYPQIAGSQQITIRDL
                     LYMRSGLHRTASPTIPMTDEEVVQFAIQHLKFTDYQTSHYDSLNFTILTGILIKLTNQ
                     PYEKLLTDDIIEPLGLQHTKFYDQVKNSSNHALSYQMSKEDDYFKPLTESETEIHNEL
                     GTGNISMSVYDLNTLFTQVLSGKLVSKDLLFSLWRENANGHPYSGGVYSGDNYILSQG
                     NIDSFDTSAIFSKDSQDAIIMESNIKADKTIKLPTADLRNQIYDLMEGTTKLANS"
     gene            2120..3295
                     /gene="mltG"
                     /locus_tag="ACG842_002220"
     CDS             2120..3295
                     /gene="mltG"
                     /locus_tag="ACG842_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HFQ0248738.1"
                     /translation="MSGNKQHPSKDKKEASKAATADIIDKRRNLRKKEDKIVRKIILV
                     IALTLLIIGGFLGFTVYRYVDSGLKPLDKSDDQLVQVEIPSGSSNKQIGEILEKDNII
                     KSGIVFNYYTKFKNLTGFQAGYYQLAPNMTLDEIGKQLQEGGTSEPTKVADGKIAIPE
                     GYDIDQIAERVAKVTGKDKKEFLDLVNDETFFNELHQKFPELLESASKAENTKYRLEG
                     YLFPATYDYYTSTSLKDLVIEMVNKTNTVMQNYYSAIKQKNLTVQEVLTLASLVEKEG
                     VKENDRKNIAQVFFNRIKANMPLQSDISVLYALGEHKELVTYEDTAVDSPYNLYTNTG
                     YGPGPFDNPSEEAIKAVLEPAENDYYYFVADTSTGNVYFAKTYEEHMELVEKYVNNS"
     gene            3431..3910
                     /gene="greA"
                     /locus_tag="ACG842_002221"
     CDS             3431..3910
                     /gene="greA"
                     /locus_tag="ACG842_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HFQ0248739.1"
                     /translation="MVEKVYPMTLEGKEKLEKELEELKTVKRGEIIERIKIARGFGDL
                     SENSEYESAKDEQAFVEGRITTIENMIRFAQIIDNDGVDADEVSIGKTVTFIELPDGE
                     EEEYTIVGSAEADPFSGKISNDSPIAQALIGKRKNDEVTIATPGGDMLVKIVKVEQA"
     gene            4079..4810
                     /gene="liaF"
                     /locus_tag="ACG842_002222"
     CDS             4079..4810
                     /gene="liaF"
                     /locus_tag="ACG842_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein
                     LiaF"
                     /protein_id="HFQ0248740.1"
                     /translation="MNSSWRFFVVVEALLLLFAVWQIVNNTGLLLLVIFGIFNIYLAM
                     RKHPRTKFQNFQLILGSLVIFFSLVNSPALWMMAVFAVLFIGLKGVEISGIDLTKNAF
                     WRKKQIMMIQTEQLKTHNNERKRQQLFGNQRIGNDVYEWDDINIAVISGDTIIDLGNT
                     LLPKDDNIVIVRKGIGRTRILVPLGVAIRLEHATLVGNVLFEEEQFSLKNEQIKIYSS
                     DYDENPRRLKIITNTLIGDVEVIRI"
     gene            4807..5874
                     /gene="liaS"
                     /locus_tag="ACG842_002223"
     CDS             4807..5874
                     /gene="liaS"
                     /locus_tag="ACG842_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     LiaS"
                     /protein_id="HFQ0248741.1"
                     /translation="MMGKISRAMLAVYSGIAAFLIILFSLFTYFYASNQSHWWGELLR
                     ARLLYVPLIFHLLAISLGVGLIVFLLLSLIQKTKYGKIEEKLRALSSGNYESKLLLLP
                     IPSASDDLYIKDIDKEITKIKEKMIEVSSELQIVTSRPQYVDGQTKEEILELERHRLA
                     RELHDSVSQQLFAAMMMMSALTEQAEKSETPEMFRKQLKMVAEIINASQSEMRALLLH
                     LRPVNLEEKSLKQGIEQLLKELQNKIQISLKWDVEDVKLSSSIEDHLFRIVQELLSNT
                     LRHAKANELEVYLHKIDNNLLLRIIDDGTGFNMNETKTGSYGLNNIKERVAGIGGTVK
                     IISFKGQGTSVEIKVPLMKEA"
     gene            5881..6513
                     /locus_tag="ACG842_002224"
     CDS             5881..6513
                     /locus_tag="ACG842_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFQ0248742.1"
                     /translation="MIKVLLVDDHEMVRLGVSFYLSIQEDIEVIGEAENGRQGYEKAM
                     TLRPDVILMDLVMEEMDGIESTKAILKDWPEAKIIIVTSFIDDEKVYPAIEAGAAGYL
                     LKTSTAHEIADAIRATQRGERVLEPEVTTKMMEKMSRRNEPVLHEELTNRENEILMLI
                     SEGKSNQEIADELFITLKTVKTHVSNILAKLEVEDRTQAAIYAFKHGLVK"
     gene            6653..7318
                     /locus_tag="ACG842_002225"
     CDS             6653..7318
                     /locus_tag="ACG842_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HFQ0248743.1"
                     /translation="MKQNFAIVGLGRFGGSICRTLIEAGQEVLAIDSNEDRVNEYMNI
                     ATHAVVANAQDEMTLRSLGIRNFDHVIVAIGEDIQASILVTLMVKEMGVPNILAKAQN
                     EYHARVLEKIGADRVVHPERDMGIRIAHNLVSKNILDYLELSDKYSLAEIRVSNPKFF
                     NKTLAELNFRQRFDLTVVAIRRVDQTVIASPAADEYVRENDNLLVIGETGDVDILDDK
                     MNQ"
     gene            7346..7639
                     /locus_tag="ACG842_002226"
     CDS             7346..7639
                     /locus_tag="ACG842_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HesB/YadR/YfhF family protein"
                     /protein_id="HFQ0248744.1"
                     /translation="MKLTVTPKAQKWFDSEIDLPETYGIRFFGKVYGKTEVHDGFSIG
                     MSVEQPEHPVKKVEIDGTLFFIEEADEWFFKGYDLLVDYDPNLEEPTYHFKKQ"
     gene            complement(7707..8654)
                     /locus_tag="ACG842_002227"
     CDS             complement(7707..8654)
                     /locus_tag="ACG842_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HFQ0248745.1"
                     /translation="MSERIIYADRPFRDEFIEEIHTIAPGYIFKTELQPEDLQNVEIS
                     LGWNSDYQEKLLASSTLKWVQSISAGVDQLPLETFSKKHILLSNGSGIHSESITEHVL
                     GIILGYSRGLFQAQKAQSDMKWLGSSVHYQSIQGKKILIIGTGHIGKMIAKKCKALDM
                     NCYGINTSGHPAEGMEKTFSLNQLKEVLPEVDFVVNILPLTEETTGLFDQHMFEHFDP
                     KSVFINVGRGASVKTQDLVQALNNQQLSFAALDVFEEEPLPQDHPLWKMDNVLITSHI
                     AGLTPDFQKKLMAIFLTNLKSYFATHELQTNQVKLSAGY"
     gene            complement(8832..9635)
                     /locus_tag="ACG842_002228"
     CDS             complement(8832..9635)
                     /locus_tag="ACG842_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1189 domain-containing protein"
                     /protein_id="HFQ0248746.1"
                     /translation="MTTKQLIISSFIRFKDLKEARNVPFWKAIVYLLVLSIIMALPIS
                     FQVFQVLNNIQLDGQKIATRIPDFTIQNGQLQTEEKEGFIYQTNSIIFTFDPEGKRTE
                     QDISTDLMGNFVSVGMLKNKLIIALPNTGTTSALLNNNQLELPYTNESLKNLTGKQLR
                     SFLSEASIPLWIKVLTFIFSIYPSFLNLLITLLFTNIAAFLYARFRLTKATFLDCLKT
                     LIYSISLPTIIATILMVFLPSFDTSAFIAFAGIFIFAQAVKGWAKISIN"
     gene            9781..10695
                     /locus_tag="ACG842_002229"
     CDS             9781..10695
                     /locus_tag="ACG842_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HFQ0248747.1"
                     /translation="MEEGIVPTQPQRSSKKLFGVVVLNAVITLSEFIGGIMSGSLSLV
                     SDAFHNLSDTLAIIFSYGAQKMARKEANKKRTYGYQRLEILSAFINSFILIILSLFLT
                     VEAFKRFTSPEKINSHLMLTVAVIGLLANLFSVLLLRQEADESLNIKSSYLHLLSDTL
                     SSVSVIIGAILIRFFDIYWIDPVITLIISIYILIEAIMVIKKAAAILIQSAPAIDYEK
                     MEQEIEAIDGVKDVHHVHIWQYSEKIIIFDGHIDFEDQLLSEIEKIYPRIISLLKLKY
                     GITHVTIQAETKAKDQKKLIFLNKDESY"
     gene            10743..11213
                     /locus_tag="ACG842_002230"
     CDS             10743..11213
                     /locus_tag="ACG842_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HFQ0248748.1"
                     /translation="MKQYRQLKGYLEAKGYWEILVYLFFGGLATIVNFVSFALARQYF
                     DLSMALSNSISWFCSVLFAFVTNKLWVFHSKSPNFTHALIECGKFFFYRILSYGLDMG
                     AMVLLINVMNSNEYVAKIITQIIVILANYIFSKLFIFKETEVFEEEVGQSEKDD"
BASE COUNT         3799 a         1724 c         2231 g         3501 t
ORIGIN      
        1 tattcatcag tagttaacat ggtgaaagaa atgaattttc aaggaactta ttctaatgaa
       61 caaatggaaa ataaacttga agaatttgag aattttgatt atgtacctat aggctataaa
      121 aaagatcaat tgcttttgaa aaaccatcaa gttacagaag atggtcaagt tatttatagt
      181 agcttttctc caaataaaga tgaaattaca tttacgttta aatcttcaaa aggcaaaaca
      241 atagttttac ctgtattccg gtataaagga ttaacggtta ctgttaattc aaaaaatgct
      301 acagtactaa aacaaggagg tccttttgtt acagtaaaaa gtgaaagtgg aataaataaa
      361 gtaacaatca ggtattcata cacatttttt tctaaaatct cattatttat ttctataatc
      421 acgctaattg ttcttctttt ttattgcata aaattaaaaa taagaacaag ccatgaatga
      481 tttgctagca catattttag caatctaaag atactatttt agatttaata ggccctttgt
      541 caaataatgt tggtaaagag aaatcttaga ataatttgga cagaacagaa agaagaacac
      601 ctctttctgt tctgtttatt tggaattaaa gtctgtagca aaaattgatt tttagatttg
      661 tcccacactc tttcctacgt ttagtcgagt acttttttta agcaaatgct attcaaagaa
      721 gtagtatttg ctatcttttc atcatatcag ctatttgcca acttagtagt tccttccatc
      781 aggtcataga tttggtttct tagatcagca gtaggcagtt tgatagtttt gtcagctttt
      841 atattgcttt ccatgatgat agcatcctgt gaatctttgc taaatatagc agatgtatca
      901 aaagagtcga tatttccttg cgacaggata taattatctc cgctatatac cccaccacta
      961 tatggatgtc cattcgcatt ttcacgccat aatgagaaga gtaagtcttt ggaaactagt
     1021 tttccagata aaacttgtgt aaacagcgta tttaaatcat agacggacat actgatatta
     1081 ccagtcccta attcgttatg gatctctgtt tcagattctg tcagtggttt aaagtaatca
     1141 tcctctttag acatttgata ggatagtgca tgattggatg aatttttgac ttggtcataa
     1201 aattttgtat gctgtaatcc taaaggttcg atgatatcat cggtcaataa tttttcatac
     1261 ggttgatttg ttaatttgat caaaattcca gtcaatatgg taaagttcaa tgaatcataa
     1321 tgagaggtct gataatctgt aaattttaag tgctgaatcg caaattgaac gacttcttca
     1381 tcagtcatcg gtatggttgg agaagcagtt cggtgcaagc ctgagcgcat atacagcaag
     1441 tcacggatag taatttgctg gctgccagca atttgtggat agaattgagc cagcttagta
     1501 tctaaagaaa ggtgcccacg ctcgacttgt tttaaaatca aaagagtagt caatgctttt
     1561 tgaaccgaac caatctgata ataaagagaa ggatcatttg gttgttgcgt gagtttattc
     1621 gcatagccat agcctttttc catgatgatg cgattatttt tctttacgta aattgagcca
     1681 atataatcat tttttcttaa tagttgatcg attttttcaa cgagctgttg ttcattggta
     1741 gtcagtatca actttttttg agctgattga gcatgggcaa ctatctgaga gtgttcatta
     1801 ttgtaaaatt gccatccaag aatagttcct gtagagaacg cactaaggct aaacaacacg
     1861 acaacgatca ttaaaaatga atattttctt tttttagcat gttttccttt tttcataata
     1921 gtttatttag tctcctttct tttttacaga aagaataaag ctaaaatagc ttgtatcttg
     1981 ataacattta ttttaatctt atagtaatgg atttactact tacatttttg atagaatatt
     2041 taaataaaat cattcacgcc aattcaaata tcatgtataa tgaataaagt tacgaacttg
     2101 atagtaagga ggagttgttt tgtcaggaaa caaacaacac ccaagtaaag ataaaaaaga
     2161 agccagtaag gcagctacag cagatataat cgacaagcgg agaaatctgc gtaaaaaaga
     2221 agataagatt gtgaggaaga tcattttagt gattgctctt acattattga ttattggcgg
     2281 atttcttgga tttaccgtgt atcgttatgt agatagcggg ttgaagcctt tagataaatc
     2341 agatgaccaa ttagtccaag tcgagatccc gagtggctct tctaacaagc agattggtga
     2401 gattttggag aaggacaata ttattaaaag cgggattgtt ttcaattatt atacaaaatt
     2461 taaaaattta actggctttc aagctggcta ttatcagtta gcgcctaata tgactcttga
     2521 cgagattggc aaacagttgc aagaaggcgg tacttctgag ccaacaaaag ttgcagatgg
     2581 gaaaattgcc attccagaag ggtacgatat cgatcagatt gctgaacggg tagcgaaggt
     2641 aactggtaaa gacaagaaag aatttttaga tttagtgaat gatgagactt tctttaacga
     2701 gttgcaccaa aaattcccag aactgttgga aagtgcatct aaagctgaga acacaaaata
     2761 ccgtttggaa ggttacttat tcccagccac atatgattat tacacgagta cttcgcttaa
     2821 ggatctagtg attgaaatgg tgaacaagac gaatacggtc atgcaaaact attattcagc
     2881 tatcaagcag aaaaatttga ctgtacaaga agttttaact ctagcttctc tagttgaaaa
     2941 agaaggcgta aaagaaaatg atcgtaaaaa cattgcacaa gtcttcttta accgaatcaa
     3001 agcaaatatg ccacttcagt cagatatctc tgttttatac gcattaggtg aacacaaaga
     3061 attagtgacg tatgaagata cagctgtaga ttcaccatat aatttgtata ccaataccgg
     3121 ctatggacct ggaccttttg acaatccaag cgaagaggca atcaaggcag tattagaacc
     3181 agcagaaaat gactattatt atttcgtagc agatacgagc actggaaatg tttatttcgc
     3241 taaaacttat gaagaacata tggaattagt ggaaaaatat gtaaataatt cttagaaaaa
     3301 caggctctag acattgatca tttttagaaa aacatttaca tttgaagaaa agcgtggtaa
     3361 tcttttttgg actatattcc tcaaaagatt accttttgcg gtattttatc gaaaaaagga
     3421 gacgttacac atggtagaga aagtttatcc aatgactcta gaaggtaaag aaaaattaga
     3481 aaaagaatta gaagaactaa aaacagtcaa acgcggagaa atcattgaac gaatcaaaat
     3541 tgcccgcggt ttcggagatc tttccgaaaa ctctgaatat gaatcagcga aagatgaaca
     3601 agcatttgtt gaaggaagaa tcacaacaat tgaaaatatg attcgttttg ctcaaatcat
     3661 cgataacgac ggtgtagatg cagatgaggt atcaattggt aaaacagtta cattcatcga
     3721 actaccagat ggagaagaag aagagtacac gattgtcgga agcgctgaag cagatccgtt
     3781 ttctggcaaa atttccaatg attctcctat tgcgcaagca ttgattggca aacgtaaaaa
     3841 tgatgaagta actattgcta ctccaggtgg cgatatgtta gttaaaatcg tcaaagtaga
     3901 acaagcgtag tctgactgaa agatctagag agttcatctt actgatgata cactctagat
     3961 ctttttctct ttgtgtattt tagatttcaa caaacgatct tacatctaaa gtcctatagc
     4021 caacagttaa aaaaataggt atgatgaaat gagaatgtgt caggagggag aaagagcgat
     4081 gaatagttct tggcgttttt ttgtggtagt tgaagcacta ctgctgttat ttgctgtttg
     4141 gcaaatcgta aacaatactg gacttttgct tttagtgata ttcggtattt tcaatatata
     4201 tctagctatg cgcaaacatc ctcgtactaa atttcaaaat tttcaattga tattaggaag
     4261 tttggtcatt ttttttagct tagtcaacag tcctgcttta tggatgatgg ctgtatttgc
     4321 cgttttattt attggtctga aaggtgtaga gatttcaggg atagatttaa ccaaaaatgc
     4381 tttttggcga aagaaacaga tcatgatgat ccaaacagag caattaaaaa cacataataa
     4441 tgagcggaaa agacagcaat tattcggtaa ccaacgtatt ggaaacgacg tctatgaatg
     4501 ggacgatatc aacatcgccg tcatttctgg tgatactatc atagatttgg gcaatacgct
     4561 tttgccaaaa gatgataata ttgtgattgt aagaaaaggg ataggcagaa cacggatctt
     4621 agtacctttg ggcgtagcca tccgattaga gcatgcgaca ttagtaggaa atgtcttgtt
     4681 tgaagaagag caattttctt tgaaaaatga acaaatcaaa atctacagca gtgattatga
     4741 tgaaaaccct cgtagattga agattattac taataccttg attggagatg tcgaggtgat
     4801 acgaatatga tgggaaaaat atccagagcg atgctagctg tttattcggg gattgcggct
     4861 ttccttatta ttctattttc acttttcact tatttttatg ccagcaatca aagtcattgg
     4921 tggggagaat tactgcgtgc acgtttatta tatgttccgc ttatcttcca tttgttagcc
     4981 atttccttag gtgtaggatt aattgtcttt ctattattat cacttattca aaagacaaaa
     5041 tacgggaaaa tcgaagaaaa gctgcgtgca ctttcttctg gcaattatga atccaaattg
     5101 ttgcttcttc cgatcccaag tgcatcggac gatttataca tcaaagatat tgataaggaa
     5161 atcactaaga taaaagaaaa aatgattgaa gtatctagtg aattacaaat tgtaacgagc
     5221 cgtccccaat atgtagatgg acaaaccaaa gaagaaattt tagaattaga aagacatcga
     5281 ttagcccgtg agctgcatga ttcagtttcg caacaattgt ttgcagcaat gatgatgatg
     5341 tcggcattga cggagcaagc agaaaaaagc gagacaccag aaatgtttcg caagcagttg
     5401 aaaatggtag cagagatcat caacgcttcc cagtctgaga tgcgtgcgct gcttcttcat
     5461 ctacgtccag tcaatttaga agagaaaagt ttaaagcaag gtatcgagca gttattgaag
     5521 gaattgcaaa ataaaatcca gatttcgctg aaatgggatg tagaagatgt aaaactatct
     5581 agttccattg aggatcactt gttccgaatc gttcaagaat tgttatcaaa tacattaaga
     5641 catgctaaag ccaatgaatt agaggtatat ttgcacaaaa tagacaataa tcttttgtta
     5701 cgtattattg acgacggaac agggtttaat atgaatgaaa cgaaaacggg aagttatgga
     5761 ttgaacaata tcaaagaacg agtagctggt attggtggaa cagtaaaaat cattagtttt
     5821 aaaggtcaag gtacaagtgt agaaatcaag gtccctttga tgaaggaggc atagataagg
     5881 atgataaaag ttttattagt agatgaccat gaaatggtgc gcttaggcgt ctctttttat
     5941 ttatctattc aagaagacat tgaagtaatt ggagaagctg aaaatggacg acaaggctat
     6001 gagaaggcga tgacacttcg accggacgtc attttaatgg acttagtcat ggaagaaatg
     6061 gatggtatcg aatcaacaaa agcaatctta aaagattggc cagaagctaa aatcattatc
     6121 gtaaccagtt ttattgatga cgaaaaagtt tatcctgcca ttgaagcagg tgcagcaggg
     6181 tatttgttga agacctctac cgcacatgaa attgctgatg ctataagggc aacgcaacgt
     6241 ggcgagcgag tattagaacc agaagtgaca acaaaaatga tggaaaaaat gagtcggcga
     6301 aatgagcctg tattacatga agaactaacg aatcgggaaa atgaaatttt aatgctgatt
     6361 tctgaaggaa aaagtaatca ggaaatagcg gacgaattgt ttattacact gaaaacagta
     6421 aaaacacatg tttccaatat attggctaag ctagaagtgg aagaccgtac acaagccgct
     6481 atctatgcat tcaaacatgg tttggtgaaa taaaaacgat gataaagagg ataatcaaaa
     6541 gctgagaaaa taactattat ttttcttggc ttttttacaa taatgatgct tgcttttgtt
     6601 tttgtaaagg ctttgttata ctgagaaaag aagatactaa aggaggaaaa cggtgaaaca
     6661 aaattttgca attgtaggtc ttggacgatt tggcggaagt atctgccgaa cactgatcga
     6721 agctggtcaa gaagtactgg ctattgatag taatgaagat cgggtcaatg aatatatgaa
     6781 tatagccact catgctgttg tggcaaatgc acaggatgaa atgacacttc gatcattagg
     6841 cataagaaat ttcgatcatg taattgtcgc tattggagaa gatattcaag ccagtatctt
     6901 agttactctg atggtaaagg aaatgggcgt accaaatatt ttagcaaaag cccaaaatga
     6961 gtatcatgcc agagtacttg aaaagatcgg agctgatcgc gtcgtccatc ccgagcgaga
     7021 catgggaata aggattgcgc ataatttagt gtcaaagaat attttagatt acctagagtt
     7081 gtcagataaa tattctttag cagaaatccg agtctccaat cctaaatttt tcaacaaaac
     7141 attagctgaa ttaaatttca ggcaacgttt tgacttaaca gtagtcgcca tacgaagagt
     7201 agatcaaacg gttatcgctt ctcctgcagc agatgaatat gttcgtgaaa atgataatct
     7261 actggttatt ggagagacag gcgatgtgga tattctagac gataagatga atcaatagaa
     7321 agaaaagaga aagtaggttg ttgggatgaa attaacggta acgccaaaag cacaaaagtg
     7381 gtttgactca gaaattgatc tgccagaaac ttacggaata cgtttttttg gaaaagtcta
     7441 cggcaaaaca gaggttcatg atggtttttc gattggaatg tcagttgaac agccagaaca
     7501 tcctgtaaag aaagtggaaa ttgatgggac actttttttt atcgaggaag cagatgaatg
     7561 gttctttaaa ggatatgatt tacttgttga ttatgatccc aacctggagg agccaaccta
     7621 tcactttaaa aaacaataaa aaaagagagg gtgacacaat ttttgtcacc ttctcttttt
     7681 tcttttgaaa atattgtttt cagctattaa tatcctgcag acaattttac ttgattggtt
     7741 tgaagctcat gggtggcaaa gtagcttttc agattggtta aaaaaatagc cattaatttc
     7801 ttttgaaaat caggtgtcaa acctgcaatg tgggaagtaa tcagtacatt gtccattttc
     7861 caaagaggat gatcttgtgg aagaggctct tcctcaaaaa cgtctaatgc tgcaaaggaa
     7921 agttgctggt tatttaaagc ctgcaccaga tcttgtgtct taacagaagc accacggcca
     7981 acattgatga atacagattt tggatcaaag tgttcaaaca tatgctgatc aaagagccca
     8041 gttgtttcct ccgttagagg taagatattg actacgaaat ctacttcagg aagaacttcc
     8101 tttagttgat ttaaagaaaa agtcttttcc attccttctg ctggatgacc ggatgtattg
     8161 attccatagc agttcatgtc taatgctttg catttttttg caatcatttt tccaatatgg
     8221 ccagttccaa tgattagtat ttttttccct tggatagatt ggtaatggac cgatgaacct
     8281 aaccatttca tgtcactctg tgccttttgt gcttgaaaaa gaccacgaga atacccaagg
     8341 atgattccta agacatgctc cgtaatcgat tcactgtgga tgccactacc atttgaaagt
     8401 aaaatatgtt tctttgaaaa agtctcaagg ggtagctgat caacaccagc tgagatagat
     8461 tggacccatt tcaaggtaga agaggctaat agtttttctt ggtagtctga gttccagccc
     8521 aaactgatct ctacattttg tagatcctct ggttgtaatt ctgttttaaa gatataacca
     8581 ggcgctatag tatggatttc ttcgataaat tcatcacgaa atggacgatc tgcataaatg
     8641 attctttctg acatagttaa tttcccgcct ttctttactc taagtgtagc gcatacaaga
     8701 agaactttga agcgccttgc ttgttttttg agaaagaaat atttttcaaa taaaaaaggg
     8761 gctgtgacaa aaaaatgtca caacctctaa aagggtctat tgaagtagaa ataacgagtt
     8821 gtctttattt cttaattgat cgatattttt gcccatcctt tgacagcttg agcaaaaata
     8881 aagattcctg caaaagcaat gaaggcgcta gtgtcaaaag aaggaagaaa gaccatcaag
     8941 attgttgcaa ttatagtagg caatgaaatt gaatagatca atgtttttaa acaatcaaga
     9001 aaagtagctt ttgttagtcg aaagcgtgca tagagaaaag cagcaatgtt ggtaaatagc
     9061 aaagtgatca gcagattcag aaaactagga taaatagaaa atataaatgt caggactttt
     9121 atccaaagtg gtatagatgc ttcacttaaa aaagaacgga gctgcttacc tgttaagttt
     9181 tttaaagatt cattggtgta gggaagttct aattggttat tgttcaacaa agcagaagtc
     9241 gtgcctgtat taggaagagc tatgataagt ttgtttttta acatcccgac actaacaaaa
     9301 ttccccatca aatcagtaga gatgtcctgt tctgttcgtt ttccctctgg atcaaatgtg
     9361 aagataatgg agtttgtttg gtaaatgaat ccttcttttt cttcggtttg aagctgcccg
     9421 ttttggattg taaaatcagg aatgcgagta gcaatctttt gcccatccaa ttgaatgttg
     9481 tttaagactt gaaaaacttg aaaagagatt ggcaaagcca taattatact caaaactaaa
     9541 agatagacaa tggctttcca aaatgggaca tttctcgcct cctttaggtc tttgaatcgt
     9601 ataaaggaac tgataattaa ttgttttgtt gtcataagta ctcctcatct agtagtgtca
     9661 tcacttgcaa ttctagcgaa gtatcaagtg aaatacaagt gctttaagga aacagctgaa
     9721 tgattggtga aatatcaaaa aaatgttatt cttgtctaaa gactatccaa tataaataaa
     9781 atggaggaag gaatagtgcc tacacagcca cagagatcat caaaaaaact atttggagta
     9841 gtggttttga atgcggtgat cactttatca gaatttattg gagggattat gtcagggagt
     9901 ttgtctcttg tttcagatgc tttccataac cttagtgata cattggcaat tattttttct
     9961 tacggggcac aaaaaatggc ccgaaaagaa gcaaataaaa aacgtacgta tggctaccag
    10021 cgtttagaaa tattgagtgc tttcatcaac tcattcatat tgatcatatt atccttgttt
    10081 ttaaccgttg aagcattcaa gcggttcact tcaccagaaa agataaatag tcatttgatg
    10141 ctaacagtag cagttattgg attgctcgcg aatttgttct cagtattatt attacgacaa
    10201 gaagcagatg aaagtttgaa tataaaatct agttatttgc atttgttaag tgatacactg
    10261 tcttcggtaa gtgtgattat cggagcaatt ttgatccgtt ttttcgatat ctactggatc
    10321 gatccagtta ttacactgat catttcgata tatatattga tagaagcgat aatggtgatc
    10381 aaaaaagctg ccgctatttt gatacaaagt gcacctgcta tcgactatga aaaaatggaa
    10441 caagaaattg aagcaatcga tggggtaaag gatgttcatc atgtccatat ttggcagtat
    10501 agcgaaaaga taattatttt cgacggacat attgattttg aagatcagct actaagcgag
    10561 attgaaaaga tatatcctcg tataatttct ttattaaaat taaaatatgg tattacacat
    10621 gtgacgatcc aagcagagac aaaggcaaag gatcaaaaaa aattaatttt tttaaataaa
    10681 gatgaaagtt attgaaatat agagtatgat aaataagaat ttttaataag gtaggttggc
    10741 tcatgaagca gtatcgacag ttaaaaggat atttagaagc aaaaggatat tgggagatac
    10801 tagtctatct cttttttggt ggtttggcta cgattgtaaa ttttgttagt tttgctctcg
    10861 cacgccaata ttttgattta agtatggcct tatccaattc tatttcgtgg ttttgttctg
    10921 tattgtttgc ttttgtaacg aacaaacttt gggtattcca ttcaaaatca ccgaatttta
    10981 cacatgcact tattgaatgc ggtaagttct tcttctaccg tatcctttct tacggattgg
    11041 acatgggggc aatggtattg ttgatcaatg ttatgaacag caatgagtat gtggcgaaaa
    11101 tcatcactca aattattgtt atccttgcga actatatttt cagcaagttg tttattttta
    11161 aagaaactga ggtttttgaa gaagaagtag gtcaaagtga aaaagacgac taggcataac
    11221 aattgaagaa tttccttctc tttgttactc tttgt
//
LOCUS       DAZKLM010000089        11197 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3764
            SAMEA115706913-rid22582563.denovo.092, whole genome shotgun
            sequence.
ACCESSION   DAZKLM010000089 DAZKLM010000000
VERSION     DAZKLM010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706913
            Sequence Read Archive: ERR13196647
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11197)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11197)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287823.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287823.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,004
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,849
            CDSs (with protein)               :: 2,849
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 41 of 96
            Pseudo Genes (incomplete)         :: 52 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 15 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11197
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706913-rid22582563.denovo.092"
                     /isolate="VRE3764"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(13..897)
                     /gene="ftsX"
                     /locus_tag="ACG842_002231"
     CDS             complement(13..897)
                     /gene="ftsX"
                     /locus_tag="ACG842_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HFQ0248749.1"
                     /translation="MIRTFFRHLLESIKSLKRNGWMTVASVSAVTITLTLVGVFMAVI
                     MNATKLAQDIEGNVDVSVFVDIGTKDEEMKKLEKELNDLDHVKKVEFSSKEQELKKIQ
                     NEMGDSWKLFEGDNNPLYDVYVVSATDPSYTKQVAEEAADLKNVSRSDYGGASSDRIF
                     QIAGAVRTWGLVAAALLLFVAMFLISNTIRITILSRQREIQIMRLVGAKNGYIRWPFF
                     LEGGWIGLIGAILPILIITFGYAWFFQLINPSLLRSHYSLIRPQNIVWKINLLMVGIG
                     VIIGSLGSIISMRRFLKF"
     gene            complement(890..1576)
                     /gene="ftsE"
                     /locus_tag="ACG842_002232"
     CDS             complement(890..1576)
                     /gene="ftsE"
                     /locus_tag="ACG842_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HFQ0248750.1"
                     /translation="MIEMKDVMKKYSNGTTAIRNLSIVIEQGEFVYVVGPSGAGKSTF
                     IKLMYREEKASKGTLNVAGHDLIAIKNREVPYLRREIGVVFQDYKLLPRKTVYENVAY
                     AMQVIGRKPRDIKKRVMEVLDLVGLKHKVRVFPSELSGGEQQRVSIARAIVNTPKVLI
                     ADEPTGNLDPENSWEIMKLLDRINAQGTTVVMATHNSTIVNTIRHRVIAIENGRIIRD
                     QAEGEYGYDD"
     gene            complement(1681..2782)
                     /gene="prfB"
                     /locus_tag="ACG842_002233"
     CDS             complement(join(1681..2709,2711..2782))
                     /gene="prfB"
                     /locus_tag="ACG842_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_139124933.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HFQ0248751.1"
                     /translation="MEISEIRNLLTEMQEKVTSFRRSLDLDQLEEDIAEAEAKMAEPG
                     FWDDSEAAQKVISENNANKETYDQFHSLAEELEEIELMVEMLQEEPDAAMQEEAETRI
                     NALDEKMKTYELSMLLDGPYDRNNAIVELHPGAGGTESQDWGSMLLRMYTRWAEQHGY
                     QVETLDYQAGDEAGIKSVTLLIKGHNAFGYLKSEKGVHRLVRISPFDSAKRRHTSFCS
                     VDVMPELDETIDIEINPDDLKIDTYRASGAGGQHINKTESAVRITHLPTGTVVASQAQ
                     RSQLKNREQAMGMLKAKLYQLEVEKKEQEAAALRGEQKEIGWGSQIRSYVFHPYSMVK
                     DHRTNVETGNVQAVMDGDLDLFIDGYLKMHLQ"
     gene            complement(2908..5442)
                     /gene="secA"
                     /locus_tag="ACG842_002234"
     CDS             complement(2908..5442)
                     /gene="secA"
                     /locus_tag="ACG842_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HFQ0248752.1"
                     /translation="MANLIRSLIENDKKELKRLDSIAKKVEAYADQMAALTDEQLQAK
                     TAEFKGRYQKGETLDQLLPEAFAVVREAAKRVLGLYPYHVQLMGGIVLHDGNIPEMRT
                     GEGKTLTATMPVYLNALTGEGVHVVTVNEYLATRDSTEMGELYNFLGLTVGLNINSKS
                     SDEKREAYNCDITYSTNNELGFDYLRDNMVVYRHQMVQRPLNYAIVDEVDSILIDEAR
                     TPLIISGQAEKSTALYTRADNFVKRLKEEEDYKIDVQSKTISLTEAGIEKAEETFGLD
                     NLYDIENTALTHHLDQALRANYIMLLDIDYVVQDGKVLIVDQFTGRIMDGRRYSDGLH
                     QAIEAKEGVEIEDETKTMATITFQNYFRMYKKLAGMTGTAKTEEEEFREIYNIQVYQI
                     PTNRPVIRDDRPDLLYPTLQSKFHAVVQDIKDRYRKGQPVLVGTVAVETSELLSDMLN
                     QERIPHEVLNAKNHFKEAEIIMNAGQKGAVTIATNMAGRGTDIKLGLGVRELGGLAVI
                     GTERHESRRIDNQLRGRAGRQGDPGMSQFYLSLEDDLMKRFGSERIKAFLDRMKIGEE
                     DAVIQSKMLTKQVESAQKRVEGNNYDTRKNVLQYDDVMREQREVIYKQRQEVIMEEKS
                     LSKPLMNMVKRTISRVVDAHTQLEKENWNLESIIDFAGSTLVHEDSITLADIEGKTSQ
                     EIKEYLNERAQEVFNTKAAQLNGPEQLLEFEKVVILRVVDSKWTDHIDAMDQLRQSIG
                     LRAYGQNNPLVEYQTEGYKMFEDMVGAIEYEVTRIFMKAEIRQNVQREQVSQGTASHS
                     EDGDAKENSNTSAKKQPIHVNKVGRNDPCPCGSGKKYKNCHGKNAN"
     gene            complement(5664..6215)
                     /gene="raiA"
                     /locus_tag="ACG842_002235"
     CDS             complement(5664..6215)
                     /gene="raiA"
                     /locus_tag="ACG842_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HFQ0248753.1"
                     /translation="MFRYNVRGENIEVTEAIRDYVEKKVGKLERYFSDAPDATAHVNL
                     KVYTEKTAKVEVTIPLPYLVLRAEETSPDLYASIDLVVDKLERQIRKFKTKINRKSRE
                     TGMNTADAAALFNEEDENENDSELDIVRTKRLSLKPMDSEEAVLQMNMLGHNFFIFED
                     AETNGTSIVYRRKDGKYGLIETD"
     gene            complement(6343..7062)
                     /locus_tag="ACG842_002236"
     CDS             complement(6343..7062)
                     /locus_tag="ACG842_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HFQ0248754.1"
                     /translation="MRCSCCSKDIFQNLTFSEIIGVSKYRLPQTCSVCRAQFHLLETR
                     GCSGCQKPIHFNLESTKKKVKSIPVYCSDCQEWKKKYPTYDFQHRAFFVYDENMKAWL
                     NQYKFMGDIRLAGTFAYKWTQIKKEYPTYIYCPIPLSEKRRTERGFNQVSEMLKAARI
                     PFQMLLKREKHLLAQAKNTRKERLEMPQPFELAVTFSKIQNKKILIIDDVYTTGQTLF
                     HAADCLQVAQPKIIRTISLAR"
     gene            complement(7059..8372)
                     /locus_tag="ACG842_002237"
     CDS             complement(7059..8372)
                     /locus_tag="ACG842_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HFQ0248755.1"
                     /translation="MNHFWGRRLLTRDIEAIDCEEVNLPNYEKIAAVERLGNWILCHR
                     CGMRTSVIDGQLADYGYFCTHCLYLGRCDTQQDLYLFDQPEAKPREVVFFWTGQLTEK
                     QTEISKRILCHSEKRHHLIWAVTGAGKTEMLYPILVETLKAGGRVAICTPRVDVCNEL
                     FLRYRYVFPEEKITLLHGNTATAYTFTSFVICTIHQLYRFYRSFDLIVIDEIDAFPYN
                     GDAGLAHAVQTAIKPDGRFIYLSATPDKKLLKEIKQTFDIHKLALRFHRRLLPEPVLV
                     FMNNWRQKCKNKKKIKSLVRIIHQLIKENHILLFCPSITMMHRLKATLQEALPRYEIT
                     DVSSRDNQRAEKVQKMREAKYDILLTTMILERGVTFERISVIVLGADHPVFTKSSLVQ
                     IAGRADRKGPYTNSQVYFFYEEKTAAIRKACQEIKEMNEEGKKML"
     gene            complement(8453..8773)
                     /locus_tag="ACG842_002238"
     CDS             complement(8453..8773)
                     /locus_tag="ACG842_002238"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFQ0248756.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEAKGIDSDIFAVSASDA
                     DNNLANKDVDVLLLGPQVRFMKADFEKRLEPKGIPLDVINMADYGMMNGEKVLDQAIT
                     LIGE"
     gene            complement(8881..9168)
                     /locus_tag="ACG842_002239"
     CDS             complement(8881..9168)
                     /locus_tag="ACG842_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0248757.1"
                     /translation="MQERMKKYDAITHYLKNNGGSQVTLTFTQFDELLFPSNGLPKTA
                     RESTDWWANDYKHPEKGAYGWINAGYEVVVINLDKEYVVFNKLVKSSWLFD"
     gene            9295..9939
                     /locus_tag="ACG842_002240"
     CDS             9295..9939
                     /locus_tag="ACG842_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HFQ0248758.1"
                     /translation="MIERYRTIKENGQSEIEIKKSRFICSLRRIESEDQAKSFIQEIK
                     KEHWKANHNCSAFIIGEKNEIQRSSDDGEPSGTAGVPMLEVLKKNQLINVAAVVTRYF
                     GGTKLGTGGLIRAYTHAVSQALSDIGIVEGTLQQEISVMITYPQLGKLQNYLELQHFF
                     IKEIQYTDQVSVICAVDEKSADHFKESIIELLNGQVQFEEGSVSYHEQLITDHI"
     gene            complement(9998..10705)
                     /locus_tag="ACG842_002241"
     CDS             complement(9998..10705)
                     /locus_tag="ACG842_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFQ0248759.1"
                     /translation="MSNQLPVYIQIHDQLKNEIEQGIWKVGDRLPSERELAVKFNVSR
                     MTLRQAIQNLADEGILERKIGSGTYVAREKVQEKMSGATSFTEIMESQNRVPSSRTIS
                     YFLTSPSSSEMEKLNLSKDDTILRMERIRFADDVPICFEVASVPQKLIDGYSKAEITE
                     SLYRTLEEKGGKKIGPANQTITAVLASEKIADYLEVKRGDAVLRLRQISYFEDGTPFE
                     YVRTQYVGNRFEFYLEK"
     gene            complement(10925..11095)
                     /locus_tag="ACG842_002242"
     CDS             complement(10925..11095)
                     /locus_tag="ACG842_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0248760.1"
                     /translation="MKWKEKVDEAAEKLYDLVKSEKYNIEVNIPKKGGKAVQVKSKRP
                     TNHTKKWMAKNR"
BASE COUNT         3053 a         2430 c         1956 g         3758 t
ORIGIN      
        1 tatattgtgg tattagaatt tcaaaaaacg tctcatagaa atgatcgagc caagtgaacc
       61 aatgatcaca ccaatgccga ccattaaaag attgattttc caaacgatat tttgtggacg
      121 aatcaatgaa taatgggaac gtaataatga tggattgatc aattggaaaa accatgcata
      181 accaaatgtg atgatcaaaa tcggaagaat cgcaccgatc agaccgatcc agccaccttc
      241 aaggaaaaac ggccagcgaa tatatccatt ttttgcaccg actaaacgca tgatttgaat
      301 ttcacgctgt ctagacaaaa tcgtgatccg aatcgtgttc gaaatgagaa acatcgctac
      361 gaacagcagc aatgccgctg caactaatcc ccatgtccga actgcaccag cgatttggaa
      421 aatccgatct gaggaagctc caccgtaatc agaacgagaa acattcttca aatctgctgc
      481 ttcttctgct acttgttttg tataagaagg atcagtagca cttactacat acacatcata
      541 taacgggtta ttgtcacctt caaacaattt ccaggaatca cccatttcgt tttggatttt
      601 tttcaattcc tgttctttgc ttgaaaattc aactttttta acatgatcta agtcattcaa
      661 ttctttttct agctttttca tctcttcgtc ttttgtaccg atatctacga atacagatac
      721 atcaacgttt ccttctatat cctgagctaa tttagttgca ttcatgataa ctgccatgaa
      781 tacaccgact aaagtcaacg tgatcgttac cgcactgact gaagcaactg tcatccagcc
      841 gttacgtttc aaactcttga tactttctaa taaatggcgg aaaaaggttc taatcatcgt
      901 atccatattc cccttccgct tgatctcgaa taatgcggcc gttttcgata gcaattactc
      961 ggtgccgaat cgtatttacg atcgtactgt tatgtgtagc catcacgaca gtagttcctt
     1021 gggcattgat ccgatctaat aatttcatga tttcccatga gttttctgga tccagatttc
     1081 ctgtcggttc atccgcgata agtactttag gcgtattcac gattgcccga gcaatagaga
     1141 cacgttgctg ttccccaccg gataattcgc taggaaagac tcgaactttg tgtttcaaac
     1201 caacaaggtc cagcacttcc attactcgtt ttttgatatc tcgaggtttt ctgccgataa
     1261 cttgcatagc gtacgcaaca ttttcatata ctgtttttct tggtaataat ttatagtctt
     1321 ggaacaccac gccgatttct ctgcgtagat aaggaacttc gcggtttttg attgctatta
     1381 gatcatgacc tgcgacgttc aaagttcctt ttgaagcttt ttcttctcga tacatcaatt
     1441 tgatgaatgt agatttaccg gctccagacg gaccaaccac atagacaaac tctccttgtt
     1501 cgatgacaat cgagagattg cggatagcgg tcgtaccatt tgagtacttc ttcattacat
     1561 ccttcatttc aatcatggcg ttctctccat atctttttca gtctttagct attatagcat
     1621 ggcaatgttt caacatgctt gcaaaatctt acaattttat ttcaatttaa caatgagttc
     1681 ttattgtaga tgcattttta aatagccgtc aataaataaa tcaaggtcac cgtccataac
     1741 tgcttggaca tttcctgtct ctacgtttgt ccgatggtct tttaccatag aatagggatg
     1801 gaaaacataa gaacggatct gtgaacccca gccgatttct ttttgttctc ctcgaagtgc
     1861 agctgcttct tgttctttct tttccacttc taattgataa agcttagctt ttaacatccc
     1921 catcgcttgc tcacggtttt tcaattgtga gcgttgtgcc tgactagcta ctactgttcc
     1981 tgtaggcaag tgagtgatcc gtacagcaga ttctgtttta ttgatgtgct gaccacctgc
     2041 accacttgct cggtacgtat cgattttcaa atcatctgga ttgatttcta tatcgatcgt
     2101 ttcatcaagc tctggcatca catctaccga acaaaaagaa gtatgacgtc gtttcgctga
     2161 gtcaaaaggc gaaatccgta caaggcgatg aacacctttt tctgatttta gataaccaaa
     2221 agcgttatgt cctttaatca gcagggtcac actcttgatc cctgcttcat cacctgcttg
     2281 ataatctagt gtttctactt gatagccatg ctgctctgcc caacgtgtat acatacgtaa
     2341 aagcatgctg ccccagtctt gggactccgt tccgccagcc cctggatgca attcgacgat
     2401 cgcattgttt cgatcataag gtccgtctaa cagcatagat aattcatacg tcttcatttt
     2461 ttcatctaat gcattgatac gtgtttctgc ttcttcttgc attgctgcat caggttcttc
     2521 ttgcagcatc tccaccatca attcgatttc ttctagttct tcggcaagag aatggaattg
     2581 gtcataggtt tctttattag cgttattttc gctgatcact ttttgtgcgg cttcgctgtc
     2641 atcccaaaaa ccaggttcag ccatttttgc ttctgcttcc gcgatgtctt cttccagctg
     2701 atctaagtca aagagacctc ctgaaacttg tgaccttttc ctgcatttct gtcaggagat
     2761 ttcgaatttc ggatatttcc atgttttttc cttctttcac tatacttttt tcattatatc
     2821 tagtctaaca gaatcagcaa ataacagaaa gaaggtgaga cgaattctta aaaaaagaat
     2881 ttgtctcacc aatcctgctg tcagaaatta gtttgcattt tttccatgac agttcttata
     2941 ttttttcccg cttccacaag gacaaggatc atttcttccg accttgttca catggattgg
     3001 ctgttttttc gcgctagtat tactattttc tttggcatct ccgtcttctg agtgagaagc
     3061 tgttccttga gaaacctgtt cacgctgtac gttttgacgg atctcagctt tcataaagat
     3121 tcgagtgact tcatactcga tggcgcctac catatcttca aacatcttat agccttctgt
     3181 ttgatattcg actaatggat tgttctgtcc ataagcacgt aagccgattg actgacgtaa
     3241 ttgatccatt gcatcaatat ggtctgtcca tttagagtcc acaacacgca agatcacgac
     3301 tttttcaaat tccaacagtt gctctggacc atttagttga gccgctttgg tattaaatac
     3361 ttcttgtgct cgttcgttta gatactcttt gatctcctga gatgtttttc cttcgatatc
     3421 tgcaagagta atactatctt catggacaag agtactgccg gcaaaatcga tgatactttc
     3481 caaattccaa ttttctttct ctaattgtgt atgcgcatct actacacgac tgattgtccg
     3541 tttgaccatg ttcattagtg gcttggataa gcttttttct tccatgatta cttcttgtcg
     3601 ctgtttatag attacttcac gttgttcacg catcacatca tcgtattgta agacgttttt
     3661 acgtgtgtca tagttatttc cttctacacg tttttgtgca gattctactt gtttcgtcag
     3721 catcttgctt tggataacag catcttcctc accgatcttc atccgatcta agaaagcttt
     3781 gattcgttct gaaccgaatc gtttcatcaa atcatcttcc aatgacaagt agaattgtga
     3841 cataccaggg tctccttgac gtcctgcacg tcctcgaagc tggttatcga tacgacgaga
     3901 ttcatgtcgt tctgtcccga taactgctag accaccaagt tctcttaccc caagtcctaa
     3961 cttgatatct gttccccgac cagccatgtt ggtagcaatc gtcacagcac ctttttgacc
     4021 agcattcatg atgatctctg cttctttaaa gtgatttttc gcattcagca cttcatgagg
     4081 aatacgttct tgattcagca tatctgataa taactcagat gtttcaaccg caacagttcc
     4141 gactaacaca ggttgacctt tgcggtagcg atctttgata tcttgcacga cagcgtggaa
     4201 cttgctttgt aatgttgggt aaagtaaatc tggacgatca tcacggataa ccggacgatt
     4261 tgtcggaatc tggtatactt ggatgttata aatttctctg aattcttctt cttctgtttt
     4321 agctgtacca gtcattcccg caagtttctt gtacatacgg aaatagttct ggaaagtgat
     4381 cgtcgccatg gttttcgttt cgtcttcgat ctctacgcct tctttagctt cgattgcttg
     4441 gtgaagtcca tctgaataac ggcgtccatc catgatccga ccagtgaact ggtcaacaat
     4501 cagtactttt ccgtcttgga caacataatc gatatctaaa agcatgatat agttggctct
     4561 caatgcttga tccaagtgat gggtcaatgc agtattttca atgtcgtata agttatccaa
     4621 accgaatgtt tcttctgctt tttcgatccc tgcttcggtt aaactgattg tttttgattg
     4681 tacatcgatt ttataatctt cttcttcttt caaacgtttc acgaagttgt ctgctcttgt
     4741 atataaagct gtggattttt ctgcttgccc tgaaatgatc agcggcgttc tcgcttcatc
     4801 aatcaagatt gagtcaactt catcgacaat ggcataattc agcggacgtt gtaccatttg
     4861 atggcggtaa acgaccatat tgtctctcaa gtagtcaaat cctaattcat tattcgtact
     4921 ataagtgata tcacaattat aagcctcgcg tttttcatcc gaagattttg agttgatatt
     4981 caaccccact gttaatccta agaaattata aagttcgccc atttccgtcg agtcacgggt
     5041 tgctaaatat tcattgacgg taactacgtg taccccttcg ccagtcagag catttagata
     5101 tactggcatc gttgcggtca aggttttacc ttcaccagta cgcatttcag ggatatttcc
     5161 atcgtgaagc acgattccac ccatcaattg tacgtggtat ggatataacc ccaatacacg
     5221 ttttgctgct tctcggacga ctgcaaaagc ttccggaagt aattgatcta atgtttctcc
     5281 tttttggtat ctgcccttga attcagcagt ttttgcctgt aattgttcat cagttaatgc
     5341 tgccatttgg tcagcgtatg cttcaacttt ttttgcaatg ctatctaaac gttttagttc
     5401 tttcttatcg ttttcgataa gtgaacgaat caaattggcc atgcgttgtg taatctcctt
     5461 ttccttctgt gtattatcca gactatgtag actttacttt tcattttatc attagttata
     5521 gataattgaa atatcttaag cgtattaaag taagaacttc ataatttatt catatttcat
     5581 ctaaaagagg aggaaactca aaaaaacttg cggcagctgt cagtagctgc tgcaagtttt
     5641 cgttgttatt taagtaaggc tgatcagtct gtttcaatca gtccgtattt tccatcttta
     5701 cggcggtaaa cgatacttgt accatttgtt tctgcatctt caaaaataaa gaagttatgt
     5761 cccagcatgt tcatttgtaa gactgcttct tcactatcca ttggtttcaa tgaaaggcgt
     5821 ttcgtacgta cgatatctaa ttcagaatca ttttcgtttt cgtcctcttc attgaataaa
     5881 gcagcagcat ctgcagtatt catgcccgtt tcacgggatt tacgattaat ttttgttttg
     5941 aatttgcgga tttggcgttc caatttatca acaaccaaat cgatacttgc gtataaatct
     6001 ggagaagttt cttcggcacg taatacaaga taaggtaatg ggatcgttac ttccactttt
     6061 gctgtttttt ctgtataaac ttttaagttc acatgcgccg ttgcatcggg tgcgtcactg
     6121 aaatagcgtt ctagtttgcc tacttttttc tcaacgtagt cgcgaatcgc ttcagttact
     6181 tcgatatttt cgccgcgtac attatatcta aacataaaaa ttaccccttt cgtctaccac
     6241 aatcaagaag gagaaggacc actctttccc tcttcttaat tctattatat cgaactctag
     6301 gttatttgca aattaaatcg ctatcaaatt tccacatcta attcatctcg ccaaagaaat
     6361 ggttcggatg attttcggct gtgcgacctg caaacaatct gctgcgtgga aaagagtctg
     6421 tcctgttgta tagacatcat caataatcaa tattttttta ttttgtatct tagaaaaggt
     6481 gactgcaagt tcaaaaggtt gaggcatctc caatctttcc tttcgcgtgt tttttgcttg
     6541 agccagcaga tgcttctctc ttttgagtag catctgaaaa ggaattcttg ctgctttcaa
     6601 catctcgctt acttgattga atcctctttc tgttcgcctt ttttcagata aaggtattgg
     6661 acaatagatg tatgtcgggt attctttttt tatctgcgtc catttatatg caaaggtgcc
     6721 tgctagtcgg atatctccca taaacttgta ttgattgagc caggccttca tattttcatc
     6781 gtacacaaaa aaggcacggt gctgaaaatc ataggtcgga tatttcttct tccattcttg
     6841 acagtcactg cagtaaacgg gaatagactt tactttcttt ttcgtactct ctagattgaa
     6901 atggattggt ttttgacagc cagaacagcc tcttgtttcc aataaatgaa attgtgcgcg
     6961 acaaactgaa caagtctgag gtaatctgta tttcgaaaca ccaataatct cgctgaatgt
     7021 taaattttgg aagatatctt tactgcagca actgcacctc acagcatctt cttcccttct
     7081 tcattcattt ctttgatttc ctgacaagct tttcgaatag ctgcagtttt ttcttcatag
     7141 aaaaagtata cttgactgtt cgtataaggc cctttacgat cggctcttcc tgcgatttgt
     7201 accaaagaag atttagtaaa aaccggatga tccgctccta aaacgataac ggatattctt
     7261 tcaaatgtga ctccacgttc aaggatcatc gtggttaaca gtatatcata ttttgcttct
     7321 cgcattttct ggactttttc cgcccgctga ttgtctcttg aagaaacatc tgtgatctca
     7381 tatctcggaa gagcctcttg aagtgtggct ttcaaacgat gcatcatcgt gatacttgga
     7441 caaaataaaa ggatatggtt ttctttaatt aattgatgaa tgatccgcac caaagatttt
     7501 atcttcttct tgttcttgca tttttgtcgc caattattca tgaaaaccaa gacgggttca
     7561 ggaagcaaac gccgatgaaa gcgtaaagca agtttgtgga tgtcaaacgt ctgcttgatt
     7621 tcctttagta actttttatc aggtgtggca cttaagtaga tgaatcgtcc gtctggttta
     7681 atcgctgttt gcacagcatg agctaatcct gcatccccgt tataaggaaa cgcatcgatc
     7741 tcatcgatca ctattaaatc aaaggaccga taaaaacggt atagctgatg aatcgtacag
     7801 ataacaaaag aagtaaaggt ataagccgtt gctgtattcc catggagcaa cgtgattttt
     7861 tcttctggaa aaacataacg atagcgtaaa aacaattcat tacaaacatc gactcgcggt
     7921 gtacaaatcg ctactcttcc tccagctttt aaagtttcca ctaaaatagg ataaagcatc
     7981 tctgttttcc ctgcacctgt tacagcccaa ataagatgat gtcttttttc tgaatggcaa
     8041 agtattctct ttgagatttc cgtttgtttt tcagtaagct gtcctgtcca aaaaaagacg
     8101 acttctcgag gttttgcttc aggctgatca aacaaatata aatcttgttg agtgtcgcat
     8161 cgccctaaat atagacaatg tgtacaaaaa taaccgtaat ctgcaagctg tccatcaatg
     8221 acggacgtcc tcattccaca gcgatgacaa agaatccagt tgcccaaacg ttccactgct
     8281 gcgatcttct cataatttgg taaatttacc tcctcacagt ctattgcttc aatatctctc
     8341 gtcagaagcc ttctacccca aaaatgattc atattcttcg ccctaaaaaa atatacgaaa
     8401 aaacaagcga tctatttttt ggatcgcttg ttcgatagtt atactgttct atttattcac
     8461 ctatgagcgt gattgcttga tctaacactt tttcgccatt catcatacca taatcagcca
     8521 tattgatgac atctaaagga atgccttttg gttccagacg tttctcaaaa tcagctttca
     8581 taaagcgaac ttgtgggcct aataacaaca catccacgtc tttatttgca aggttgttat
     8641 ctgcgtcaga agcggacaca gcgaaaatat ccgagtcgat tccttttgct tcagcagctt
     8701 tttgcatttt tgttactagt aaacttgtgc tcataccagc cgaacataca agcatgattg
     8761 tttttttagc catacttttc tctccttttt aaaacaaact atgcaaacgt tttcttcgcg
     8821 tctgttactc tattatataa aaagaaagaa atttgtcaac gttttctaaa cgcggtttat
     8881 ttaatcaaat agccaactag atttcactaa tttattgaac acgacatatt ctttgtccaa
     8941 attgatgaca actacttcat agcctgcatt tatccagcca taggcacctt tttcagggtg
     9001 tttataatca tttgcccacc aatctgtact ttcccttgct gttttgggaa gtccgttact
     9061 gggaaacagc agttcgtcaa attgggtaaa ggtcaacgtt acttgagagc cgccattgtt
     9121 cttcaaataa tgcgtaattg catcgtactt tttcattcgt tcctgcatcc ccagatccac
     9181 ctgcctttgt ctttattatc gcatactaca gcaattgcgc aaccgttaca gtgttgaaac
     9241 aacctagaac tttatttata ctgaataaag aaaatgaaaa aaagaggtga caagatgatc
     9301 gagcgctatc gtacaataaa agaaaatgga caaagtgaga ttgagatcaa aaaatcacgc
     9361 tttatctgtt ctcttagacg aatagaatca gaggaccaag caaaaagttt cattcaagaa
     9421 ataaaaaaag aacattggaa agcgaaccat aactgcagcg ccttcatcat tggagaaaaa
     9481 aatgaaatcc agcgaagcag cgatgatgga gaaccaagtg gaacggcggg cgtaccgatg
     9541 ttggaagtcc tcaaaaaaaa tcaactgatc aatgtagctg cagtagtcac tcgatatttt
     9601 ggaggaacga aactcggcac aggcggatta atacgagcat acactcatgc ggtctctcaa
     9661 gcattgtctg acattggaat cgtagaaggt accttgcaac aggagatctc tgtgatgatc
     9721 acgtatccgc aactaggaaa attacagaat tatcttgaac tccaacattt ttttataaaa
     9781 gaaatccaat atactgatca agttagcgtc atctgtgctg tagatgaaaa aagtgctgac
     9841 cactttaaag aatccatcat tgagctgctg aacggacaag tccaatttga agagggttct
     9901 gtttcttatc acgagcaact gataactgat catatataaa aacaggtgat tctgattctc
     9961 ttttatttct aataaggaat cagggatcac ctgtttttta tttttccaaa taaaactcaa
    10021 aacgatttcc tacgtattgc gtacgcacat actcaaacgg cgtaccgtct tcaaaataag
    10081 aaatctggcg caaacgaaga actgcatctc ctcgttttac ctctaaatag tcagcaattt
    10141 tctcagaagc caacacagca gtaatcgttt gattcgctgg accgatcttt ttcccgcctt
    10201 tttcttctag cgtgcgatac aacgattcag taatctctgc tttgctgtag ccatcgatca
    10261 gcttttgtgg aacactggca acttcaaaac aaatagggac atcatctgcg aaacgaatac
    10321 gttccattcg caagatcgtg tcatctttac tcaagtttag tttttccatt tcacttgagc
    10381 ttggcgaagt caagaaataa gagattgtcc gactagacgg tactcgattt tgtgattcca
    10441 taatttctgt aaaacttgtt gcaccagaca ttttttcttg tactttttct cgcgcaacat
    10501 acgtacccga accgatcttt ctttctaaga ttccttcatc tgccaagttt tgaattgctt
    10561 gtctcaacgt catacggctg acattgaatt tcactgctaa ttctctttct gaaggaagac
    10621 ggtctccgac tttccatata ccctgttcga tttcattttt taattggtca tgaatctgta
    10681 tatatacagg taattgattc gacataacgt acacccgtcc tttcgtttct tctattataa
    10741 ctggtatata cctgattcac aactgaaaaa gataaaggtt tctatccatc aacagaggat
    10801 ttagacacac atcgttctcc ctactcctat tgtcagaaac aattttttag atatgttcaa
    10861 cctagaaaat aacaaaagac cctaaaatcg atgaaaatgc cgacttatgg atcttttgtt
    10921 attattagcg gtttttagcc atccattttt ttgtatgatt cgttgggcgt tttgatttta
    10981 cctggaccgc ttttccgcct tttttcggaa tattcacttc aatattgtat ttttctgatt
    11041 tgaccaaatc atataacttt tcagctgctt cgtctacttt ttctttccat ttcatgtgga
    11101 tccctccaat tcttgtagtt aacttaactc tatcttaatt cagcagaatc ttcaaggaac
    11161 aaggtagcaa agcaaaaaaa agagctctcg aactaac
//
LOCUS       DAZKLM010000090        10649 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3764
            SAMEA115706913-rid22582563.denovo.094, whole genome shotgun
            sequence.
ACCESSION   DAZKLM010000090 DAZKLM010000000
VERSION     DAZKLM010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706913
            Sequence Read Archive: ERR13196647
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10649)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10649)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287823.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287823.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,004
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,849
            CDSs (with protein)               :: 2,849
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 41 of 96
            Pseudo Genes (incomplete)         :: 52 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 15 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10649
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706913-rid22582563.denovo.094"
                     /isolate="VRE3764"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            34..657
                     /locus_tag="ACG842_002243"
     CDS             34..657
                     /locus_tag="ACG842_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0248761.1"
                     /translation="MFVIWEVVKSRVQEMNEHFSVSFAENPSKYFRRLVFLLILSLLL
                     TVAMFLLPEKIREEQVNSLVYSSIPTSKLKPFDYSKADQKIKQSRAISVMFSKPNGKT
                     YQDMLDVFNDPELMEELNRPIFYYPIVYDVHELEQKYNIRTDEVTFIFFDGGKEANRI
                     TAGKGGITDFDKELIPELNRLPLANIKQLEEELDRTGVTSQNSEIAN"
     gene            1107..2156
                     /gene="trpX"
                     /locus_tag="ACG842_002244"
     CDS             1107..2156
                     /gene="trpX"
                     /locus_tag="ACG842_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan ABC transporter substrate-binding
                     protein"
                     /protein_id="HFQ0248762.1"
                     /translation="MKNKRLITVVVIILVYLAGAFIYGKASGEKESTSTNQTKKTTVV
                     GVLQYVSHPALDEIYRGIKDGLKEEGLKEGENLEIKFQNGQADQSKLATMSQQLVQSD
                     PDALIGIATPAAQSLANTTSSIPVVLGAVTDPVGAGLVASLEKPGGNVTGVSDQPPVT
                     SQIKLASDLLPNAKKVGILYASSEDNSKYQVAQAEEAAKKEGLESVKYAVPSTNEIAQ
                     TVQVMSRQVDFIYVPLDNTIANAMQTVVKEANKANIPVIPSVDTMVEQGGLATIGINQ
                     YQLGLQSGKMAAELASGKEKPETTPVYTFDQGDTVINQSQADRLGIIVPQSMKEKAKI
                     ITDDSQQETSKEDDK"
     gene            2153..3034
                     /locus_tag="ACG842_002245"
     CDS             2153..3034
                     /locus_tag="ACG842_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0248763.1"
                     /translation="MIVSAISQGMLWAILGLGIFMTYRILDFPDMTTEGSFPLGAAVC
                     VTAITHGVAPIVATLLGVGAGMIAGLVTGLLYTKGKIPVILAGILVMSGLNSVILFVM
                     RTPNLSLLNHPILQDSFHRFGLPDYFDTVLLGFISLGILIAVLLFFFNTDLGQGYIAT
                     GDNETMARSLGIKTDRMKILGLTLSNGVIALSGALIAQNDGYADVNKGIGVIVIGLAS
                     IIIGEVIFQELTLAERLIAIVVGSIIYQLLILVVIKLGFDTTYLKLFSAIILAICLMI
                     PQLKKALNLKTGFEKEA"
     gene            3037..3807
                     /locus_tag="ACG842_002246"
     CDS             3037..3807
                     /locus_tag="ACG842_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0248764.1"
                     /translation="MTDILTIKNGRKVVNNGMNEEKVLLDEINLTIREGDFITVLGGN
                     GAGKSTLFNTIAGTLQLSQGSISFKDQLITKDSEEKRARFLSRVFQDPKMGTAPRMTV
                     AENLLLAQKRGQKRKFRLRNLKNQKEDFFRLCQEVGNGLEQHLDTPAGELSGGQRQAL
                     SLLMATITAPELLLLDEHTAALDPKTAKALMRLTNQRINEQKLTCLMITHRMEDALKY
                     GNRLIVLQKGKIVQDISEKEKQHLTMGDLLQFFEEVVD"
     gene            4086..4940
                     /gene="aroE"
                     /locus_tag="ACG842_002247"
     CDS             4086..4940
                     /gene="aroE"
                     /locus_tag="ACG842_002247"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HFQ0248765.1"
                     /translation="MISGQTKLAGLFADPASHSLSPLMHNTAFEANNIDAVYLAFQVN
                     PMNLKQAVESIRTFDMLGVNLSMPNKTAVIPYLDEVSQEAQLIGAVNTIVHEKGRLIG
                     YNTDGAGFMQSVQEAGISIKKKKMTLLGAGGAAKAVTVQAALDGAEEIVVYKRNNQTF
                     AQVKETFEQLSYRTSCKIKVRDFADTHQLRKDLKESCLLVNATDIGMGKKINQSPLLD
                     ISMMHENLAVFDLIYAPRETRLLKEAKKVGAKGYNGLGMLIYQGAIAFELWTKEKMPI
                     HVIEKLFD"
     gene            5118..6281
                     /locus_tag="ACG842_002248"
     CDS             5118..6281
                     /locus_tag="ACG842_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HFQ0248766.1"
                     /translation="MSRMDRYKDIHKKAKPLKRDTGSIFARRERKRTRMENEEDATRI
                     YEREQHSFEHLNQYEQQIYRDAPRAEEQQKNKKFFNRQKKNKPLKPRRKRSWKKIILS
                     ILLVLILYSGISFFIGKYVAEHDTSLEAATSETFHGQKATNGAHNILILGSDTRGEDA
                     GRADTIMVLQLDGPSHKPKLISFMRDSFVTIPGVGQNKINSAYAYGGADLVRQTLVEN
                     FGIDCQYYAKVDFKSFEKVIDALFMNGVKIDAEKDLNLDGVDIKKGVQKMDGHVLLQY
                     ARFRMDEQGDFGRVRRQQQVMNAIFSQLKNPLNLIRAPYAAGKALGYTSTDVTAWFLT
                     KNLFSIARGAGGIDRISVPVDGSWNYGQSDYAGSILEIDNQMNQSAITDFLNK"
     gene            6398..6808
                     /locus_tag="ACG842_002249"
     CDS             6398..6808
                     /locus_tag="ACG842_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFQ0248767.1"
                     /translation="MDIYLNFKNESREAIAFYEKVFQTEASGITTYGEMPGIEQMNLP
                     EEAKNLITNARLNIYDTLVMFADTPEGIGPELVVGNNVSLVIQTRNEEETKRLFESLA
                     EGGKIEMPLDAVPWAQLYGMVTDRFGINWQINQE"
     gene            6982..9210
                     /gene="topB"
                     /locus_tag="ACG842_002250"
     CDS             6982..9210
                     /gene="topB"
                     /locus_tag="ACG842_002250"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HFQ0248768.1"
                     /translation="MKTVILAEKPSQAKAYAETFQQQKRHEGYYEISDPLFAGEVTIT
                     YGFGHLVDMVAPGAYEERWAKWSLENLPIFPETFRYEVPMDKRAQFSIVKRELQSADT
                     IIIATDGDREGEAIAWSIIRQANAFSKEKNYLRLWINSLEKEAIYEGFKNLQPGKNYF
                     PKYKEAQARQNADWLIGMNGSPLYSLLLQRKGIDGSFSLGRVQTPTLYMIYQLQEEIK
                     HFKKETYFEGEGLITTNKGQFSGKIVPKKAFKTQEELTKEIEKLGAHLGKQNGRILEV
                     TKKEKRLHSPRLFSLSSLQTKMNQLMKASAKATLDAAQGLYESKFLSYPRTDSFYITE
                     NEHQYLVKHLQNYKKFLGIEEVQTTEIKAKKRYVDAKKVQEHHAIIPTKTIPTPARFA
                     KLSKLQQAIYLQVLRTTVAMFAADYIYEETTVMTGVQQLKLKSIGKIPLQQGWQEILR
                     IKSKKKEAQTLPPIVENEEAEVELKSMEKETQPPKPYTEGTLITAMKTAGKTLDNDEA
                     QEILKEVEGIGTEATRANIIENLKQKHYIEVNKNEITVTPKGITLCKAVANEPLLTSA
                     EMTARWEGYLRKIGKQEGTPSVFLENIKKFILHLLAEVPNQIDKVDFSDEISGTRQIK
                     AMEKKNDQLGRCPKCKKGIVMLYPKIATCLNPECDFKLWPTVAKKKLTKTNLRDLLSK
                     GKTSKVVKGFTGKKGKFDAVLVLKEDMTIGFSFPWIENSGTKAVDGNKETSRKNNENE
                     VE"
     gene            9381..9665
                     /locus_tag="ACG842_002251"
     CDS             9381..9665
                     /locus_tag="ACG842_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0248769.1"
                     /translation="MGAITIVAVYQAFLKYPDYGTKLLSLTHNQTAYSPTLILSTLTA
                     GAKSATYFFHKTTSMLKRKKQRKQLLVVVAAMIIIYFLPVITQVFHQYFG"
     gene            9825..10061
                     /locus_tag="ACG842_002252"
     CDS             9825..10061
                     /locus_tag="ACG842_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFQ0248770.1"
                     /translation="MKKMKKYKIRKSGNSDVTTIPPEVKEFMGVQTGDAISYVFQSDG
                     SVRMIKAQEEPDIDSLVDSIMNQYEDALKDLVDL"
     gene            10058..10456
                     /locus_tag="ACG842_002253"
     CDS             10058..10456
                     /locus_tag="ACG842_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HFQ0248771.1"
                     /translation="MRYLSTKEIIKINAKVILRHSPGEMIGVKDANALDMAVKQPSQA
                     VFNQELYPEIYEKAAILAINLAKKHPFHNGNKRTALVAMLLFLQLNNCPVAFSRQEAV
                     DFIIMITTSSLDFDSLKSKITSYLRQTAIK"
BASE COUNT         3722 a         1773 c         2253 g         2901 t
ORIGIN      
        1 taataaaaaa taggagaaaa agtagaaaag aaggtgtttg ttatttggga agtagttaaa
       61 tcgagagtac aagaaatgaa tgaacatttc tcagtgtcat tcgcagaaaa tccaagtaag
      121 tattttcgac gtcttgtttt tctgctcatc ttatcattgc tgttaacagt tgcaatgttt
      181 ttattgccgg aaaaaatccg agaagagcaa gtaaatagtc tcgtttattc gtccatacca
      241 accagcaaat tgaaaccttt tgattacagc aaagctgatc agaaaatcaa acaaagccgg
      301 gcgatatcgg tgatgttcag caaaccgaac gggaaaacct atcaggacat gcttgatgtt
      361 tttaatgatc ctgagcttat ggaagaattg aatcgaccga tcttttatta tccgatcgtt
      421 tatgatgtac atgaactgga acaaaaatat aacatacgaa cggatgaagt gacatttatt
      481 ttctttgatg gaggaaaaga agcaaatcgg ataactgcgg gtaagggagg aataactgat
      541 tttgataagg agttgatccc tgaactgaat cgacttccgt tagccaatat caaacaatta
      601 gaagaagaat tggatagaac gggagtaact tcccaaaatt ccgagattgc taattgagac
      661 attggcgcga tgaaacggtg ggtctatata gcgctatttt taattatcag aacaagtgat
      721 gtttgatctt caaaataaca aataaaaaat taaaaaatat agttgacgaa attcaaaaat
      781 tcgattagac ttaaatcaat tcttaaggaa agggtgacct gtaatgaact tatcaaaacg
      841 tatttcagca gaaaacaaaa aaaattggcg cagctggcgt agataagttg tattaatggt
      901 gagactttcc catagataca actttttaga gtgcatttct aaagcttgta tctatgctag
      961 acatgaacat tgactgccgc atagattaga atctatgcgg ttttttgtgt aaagacaatg
     1021 accgctttgg ccattgtctt tttttgtaga ctggatactt gcctgattat aataaataaa
     1081 aagattgact ttgcaggagg aaaaaaatga aaaacaaacg attaataaca gttgtagtaa
     1141 ttattttagt ttatttagca ggtgcattta tttatggaaa agcctcaggc gaaaaagaaa
     1201 gtacttcgac gaaccagaca aaaaaaacaa cagttgttgg ggtattgcaa tacgtgagcc
     1261 atccggcatt agatgagatt tatcgaggaa tcaaagatgg tctgaaagaa gaaggattaa
     1321 aagaaggaga aaatcttgag atcaaatttc aaaatggaca agccgatcag agcaagctag
     1381 ccacaatgag tcaacagcta gtacaatctg atccagatgc cttgattgga atcgcaacac
     1441 ctgccgctca atcattagca aacacgactt catcgatccc agttgtgtta ggtgctgtaa
     1501 cggatcctgt gggggctgga ttagtcgctt ctttagagaa acctggtggt aatgtaacag
     1561 gtgtttctga tcagccgcct gttacttcac aaatcaagct tgcttctgat ttgttgccaa
     1621 atgcaaaaaa agtcggaatc ttgtatgctt cttcagagga taattcaaaa taccaagtag
     1681 cacaagcaga agaagctgca aaaaaagaag ggttagaatc agtaaaatat gcggtgcctt
     1741 ccactaatga gattgcacaa acggttcaag tgatgagccg acaagtcgat tttatctatg
     1801 ttccattaga taataccatt gccaatgcga tgcaaacagt ggtaaaagaa gcaaacaaag
     1861 caaatatccc cgtcattcca tctgtggata caatggtgga acaaggtgga ctagcaacaa
     1921 tcgggatcaa tcaatatcaa ttagggcttc aaagcggtaa gatggcggca gaacttgcga
     1981 gtggaaaaga aaaaccggaa acgacgcctg tttatacatt tgatcaaggg gatacagtga
     2041 tcaatcaatc tcaagctgat cgcttaggaa tcattgttcc ccaatcgatg aaggaaaaag
     2101 ccaaaatcat tacagatgac tcgcagcaag aaacatctaa ggaggatgac aaatgatcgt
     2161 atcagctatt tcacaaggca tgctttgggc aatattagga ttagggattt tcatgactta
     2221 tcggatctta gactttcctg atatgacgac agaagggtcc ttcccattag gtgctgctgt
     2281 ctgtgtgaca gctattactc atggggtagc gccgattgta gctacattat taggcgtagg
     2341 tgctggcatg atagcaggac tcgttacggg gttactttat acaaaaggaa agataccggt
     2401 tattttagca gggattttag tgatgtcagg attgaactca gttattttgt ttgtgatgcg
     2461 aacaccaaat ctttctttat tgaatcatcc gattctacaa gacagcttcc atcgctttgg
     2521 gttacctgat tactttgaca cagtccttct tggcttcatc tctttgggca tcttgattgc
     2581 tgtactgcta ttcttcttca atacagattt gggtcaaggc tatattgcga caggagacaa
     2641 cgaaacgatg gctcggtcgt tagggataaa aaccgatcgt atgaaaattt taggtttaac
     2701 tttgtcgaac ggtgtcattg cattatccgg tgcgctgatt gcccagaacg acggatatgc
     2761 agatgtaaat aaagggattg gcgtaatcgt catcggtctt gcttccatca tcatcggtga
     2821 agtgatcttc caagaattga cgctagcaga aagattgatt gctattgttg tcggcagtat
     2881 catttatcag ctattgattt tagtagtaat caaactaggg tttgacacga cttatctgaa
     2941 gttgttctcc gctatcatct tagctatttg tctgatgatc ccgcaattga agaaagcact
     3001 gaacttaaaa acgggttttg agaaggaggc atagtaatga ctgatatatt gacaatcaaa
     3061 aatggcagaa aagtcgtaaa caatggaatg aatgaagaga aagtactatt ggatgaaatt
     3121 aaccttacaa tcagagaagg ggacttcatc actgtccttg gcgggaatgg tgctggaaaa
     3181 agcacactgt tcaatacgat cgctggtacg cttcaactca gtcaaggatc gatctctttt
     3241 aaagatcagc tgatcacaaa agattcagaa gaaaaaagag cacgattcct ttctcgagtt
     3301 ttccaagacc ccaagatggg aacggctcct cggatgacag tagcagaaaa tttgctactt
     3361 gcacaaaaaa gaggtcaaaa acggaaattc cgtttacgta acttgaaaaa tcaaaaagaa
     3421 gattttttca gactatgtca agaagtcggt aatggcctag agcaacactt ggatactcca
     3481 gcgggtgaat tatctggtgg tcaacgccaa gcattaagct tgctgatggc gacgatcaca
     3541 gctcctgagt tactattatt agatgaacat actgcagctt tagatcctaa aacagctaaa
     3601 gcattaatgc gattaacaaa tcagcggatc aatgaacaaa aattgacttg tctcatgatc
     3661 acgcatcgaa tggaagacgc cttgaaatac ggaaatcgtc tgatcgtact ccaaaaaggg
     3721 aaaatcgttc aagatatttc tgaaaaagaa aagcaacatt tgacaatggg cgaccttttg
     3781 cagttctttg aagaagtagt ggattgattt cgacagaaaa attcctttca gtagaaggac
     3841 ttgaaaactc attgacatct tcctttgatt catcctatac ttaaaacaat aaacaattaa
     3901 acatatttgg gtgttttgta ggatcacgta gtcaatgatt ttaacaatta aatagaatat
     3961 tctttgctct gacaagtaaa cgatgagaaa agaatttgct actttgccac ctagacgttt
     4021 cgtacttttt cgtatcaaac gctaagtggc tttttttatt gaagtaagaa caaaggagga
     4081 ataagatgat ttctggacaa accaaattag ctgggctctt tgctgatccg gcttctcaca
     4141 gtttatcccc attgatgcac aatactgctt ttgaagcaaa caacatcgat gcggtgtatt
     4201 tagctttcca agtaaatcca atgaatttga agcaagcagt cgaaagtatt cgaacttttg
     4261 atatgttagg tgtaaatctt tcgatgccga ataaaacagc ggttattcct tatctggatg
     4321 aggtaagcca ggaagcgcaa ttgatcggag cagtgaatac gatcgtgcac gaaaagggtc
     4381 gtttgattgg ctataatacc gatggtgcgg gcttcatgca atccgtgcaa gaagctggga
     4441 tttcgatcaa aaagaaaaaa atgactctac tgggagctgg aggtgcagca aaggcagtca
     4501 ctgttcaagc tgcgttagac ggcgctgagg aaatcgtagt ttataaacga aacaaccaaa
     4561 cattcgctca agtaaaagaa acattcgaac aactcagcta tcgaacttct tgtaagataa
     4621 aagttcgtga ctttgccgat acgcatcaat taagaaaaga cttgaaagaa agctgtcttc
     4681 tagtcaatgc aacagatatc gggatgggaa agaaaatcaa tcagtcaccg ctcttagata
     4741 taagcatgat gcatgagaat ctagcggttt ttgatttgat ttatgcacct agagaaacac
     4801 gtttattaaa agaagcaaaa aaagtaggag caaaaggata caatggactt ggcatgttga
     4861 tctaccaagg agcaatcgcg tttgaattat ggacaaagga aaagatgccg atccatgtga
     4921 ttgaaaagct gtttgattaa aaaaggataa tagataaaga tcttttattc tcgcataaac
     4981 ggtctcaaga aaagtaatcc tttatgtatc gaagcattta tttcgttatt ttttattaaa
     5041 ttatcaaaaa atgataggct tttgtcgatt ggttatcgta tcctttattt atcagtaaga
     5101 aacgaggagg aaccacaatg agtcggatgg atcgctataa ggacatccat aagaaggcga
     5161 aacctctgaa gcgggatacg gggagtattt ttgctagaag agaacgaaaa cgaacgcgta
     5221 tggaaaatga ggaagatgct acccgtattt atgaaagaga gcagcattca tttgaacatt
     5281 tgaaccaata cgagcaacag atatacagag atgcaccaag agcagaagaa caacaaaaaa
     5341 ataaaaaatt tttcaatcgg caaaagaaga acaaaccgtt aaaacctcga agaaagcgat
     5401 cgtggaaaaa aatcattcta tcgattttgc ttgttttgat cctttattca ggtatctcat
     5461 tttttattgg taaatatgta gctgaacatg acacaagctt agaggcagcg acttcagaaa
     5521 cctttcatgg gcaaaaagcg acgaatggtg cgcataatat tttgatcctt ggaagtgata
     5581 cacgcggaga agatgccggg cgagccgata cgatcatggt cttacagttg gatggtcctt
     5641 cccataagcc gaaactgatt tcttttatgc gtgacagctt cgttacgatc ccaggggtag
     5701 gacaaaataa gatcaattca gcttatgcat acggaggagc cgatctagtg cgtcaaactt
     5761 tagtagaaaa ttttggaatc gattgtcaat actatgcgaa agtcgatttc aaatcgtttg
     5821 aaaaagtgat cgatgcctta ttcatgaacg gagtgaagat cgatgcagaa aaagatctga
     5881 atctggatgg tgtagatatt aaaaaagggg tccagaagat ggatggacat gttcttttgc
     5941 agtacgcacg tttccgaatg gatgaacaag gagactttgg ccgtgtcaga cgccaacagc
     6001 aagtaatgaa cgctattttc agccaattga agaatccatt gaatctgatc cgtgcacctt
     6061 atgcagctgg taaagcttta ggatatacat ccacagatgt cactgcttgg ttcttgacga
     6121 aaaacctctt ttcgattgca cgaggagctg gtggcattga tcgaataagt gtgccagtag
     6181 acggttcgtg gaattatggg caaagtgatt atgctggcag tattcttgaa attgataatc
     6241 aaatgaatca atctgctatt acagattttt tgaataaatg atcataaaaa agatcaagaa
     6301 gaagctgcgc aaggcttttg tcatgaatca atcaaacgat tcactcgtaa aaaacgcttt
     6361 cttatggtag atttaaccaa gagaggagct gtttacattg gatatctatc tgaacttcaa
     6421 aaatgagtcg cgtgaagcca tcgcttttta tgaaaaagtt tttcagactg aagcatcagg
     6481 tatcaccaca tacggtgaaa tgcctggaat agaacaaatg aacttgccag aagaagcaaa
     6541 aaatctaatc acgaatgcac ggctgaacat ttatgataca ttagttatgt ttgcagatac
     6601 gccagaagga attggtcctg aattagttgt gggaaataat gtttctttag tcatccagac
     6661 aagaaatgaa gaagaaacga agcgcctatt tgaaagctta gctgagggtg gtaagattga
     6721 gatgccactg gatgctgttc cgtgggctca gttgtatggg atggtaactg atcgatttgg
     6781 catcaactgg cagattaacc aagagtgaca gctaaagatg aatattttct ggaaacatca
     6841 agcagcaaac ggatcatcgt ttgtttgctt gatgtttttg tgtctatttc gactatacgg
     6901 tatctttttt ttattgccag atgcgaaaat atgttccata atagagaagt ccgcaaaaaa
     6961 ttaagtaacg gaggtcagaa gatgaagacg gtgatactag ctgaaaaacc ttcacaagca
     7021 aaagcctatg ccgaaacctt ccaacagcaa aagcggcatg aagggtatta tgaaatcagc
     7081 gatccattgt ttgctggtga agtaaccatc acttatggtt ttggacattt ggtagatatg
     7141 gttgctccag gagcttatga agagcgttgg gcaaaatggt ctttagaaaa tttgcctatt
     7201 ttcccagaaa ccttccgtta cgaagttcca atggacaaac gtgcacaatt ttcgatcgta
     7261 aaacgagaat tgcagtcagc cgataccatc attattgcaa cagatggtga tcgagaaggt
     7321 gaagccatcg cgtggtcgat tattcgacaa gcaaacgcat tttctaagga aaagaactat
     7381 ttacgtttat ggatcaattc tcttgaaaaa gaagcaatct atgaaggttt taagaatctc
     7441 caacctggaa agaactattt tccaaaatat aaagaagcgc aagcaaggca gaacgctgat
     7501 tggctgattg gaatgaatgg aagtccatta tatagcttgc tacttcaacg aaaagggatt
     7561 gatggcagtt tttctcttgg tcgtgtacaa acaccgacac tctatatgat ttatcagctg
     7621 caagaagaaa tcaaacactt caaaaaagaa acgtactttg aaggagaagg tttaattacg
     7681 acaaacaaag ggcagttttc tggaaaaatc gtaccaaaaa aagcatttaa aacccaagaa
     7741 gagttaacga aggaaataga aaaacttgga gcgcatttag gtaagcaaaa cggacggatt
     7801 cttgaagtta cgaaaaagga aaagaggttg catagtccac gtttattttc gctatctagt
     7861 ttgcagacaa agatgaatca gctaatgaaa gcaagtgcca aagctacact tgatgctgct
     7921 caaggattgt acgaaagcaa gtttctaagc tatccaagaa cggattcctt ttacattaca
     7981 gaaaatgagc atcagtatct ggttaaacat ctgcaaaatt ataaaaaatt tttaggaata
     8041 gaagaagtac agacaactga gatcaaagca aagaaacgat atgtcgatgc gaaaaaagtt
     8101 caagaacatc atgcaattat tccgacaaag acgattccga ctcctgcacg ctttgcgaag
     8161 ctttccaaat tacagcaagc aatctatttg caagttttgc gcactactgt ggctatgttt
     8221 gctgcagatt atatctacga agagacgacc gtaatgacgg gcgtacagca attaaagcta
     8281 aaaagcatcg gaaagatccc attacagcaa ggttggcagg aaatcttacg catcaagtca
     8341 aaaaagaaag aagctcagac tcttcctcct atcgtagaaa atgaagaagc agaagtagaa
     8401 ctgaaaagta tggagaaaga aacacaacct ccaaaaccct ataccgaagg aacgttgatc
     8461 acagcaatga aaactgcggg aaaaacgctg gataatgatg aagcgcaaga aattctcaaa
     8521 gaagtagaag gaatagggac agaagctaca cgagccaata tcattgaaaa tcttaagcag
     8581 aagcattaca tcgaagtcaa taaaaacgaa ataactgtta cgccaaaagg aatcactctt
     8641 tgtaaggcag tagccaatga accattactg acgagcgcag aaatgactgc gcgctgggaa
     8701 gggtacttga gaaaaattgg taaacaagag gggacaccat ctgttttttt agagaatatc
     8761 aaaaaattca tcctacattt attggcagaa gttcctaatc aaatcgacaa ggtagacttt
     8821 tcagatgaga ttagcgggac acgtcagata aaagcgatgg agaagaaaaa cgatcaatta
     8881 ggaagatgtc caaaatgtaa aaagggaata gtcatgcttt atcctaaaat cgctacatgc
     8941 ttgaatccgg aatgcgactt caaattgtgg ccaacagtag ctaaaaagaa gctgactaaa
     9001 acgaatctca gagatttgct ttcaaaagga aagacaagta aagttgtcaa aggttttact
     9061 ggtaaaaaag ggaaattcga tgcagtttta gtattaaaag aagatatgac aatcggtttt
     9121 tcttttccat ggatcgaaaa cagtggaaca aaagcagttg atggaaacaa agaaacatca
     9181 agaaaaaata atgaaaacga agtagagtaa atgaaaacaa tgaaatagcg gaatatttat
     9241 tccgaaaagt gagaatataa ataccaataa aactaaaaaa tatcagtgaa tcaagcaaaa
     9301 acgttggggt aggctgattt tatatagata gcaaggagtt tttttatttg aaaaaagtgc
     9361 attcattttt aaattatctt atgggagcaa tcacgattgt ggctgtgtac caagcatttc
     9421 taaagtatcc tgattacggg acgaagttgt tgtcattgac gcacaatcaa acagcatatt
     9481 caccaacgtt gattttatcg actttgactg caggagctaa aagcgctact tatttttttc
     9541 ataaaacgac aagcatgcta aagcgaaaga aacagcgaaa acaattgttg gtggtggtag
     9601 cggctatgat cattatttat tttctaccag ttattacaca agtatttcat caatattttg
     9661 gttaagaata cgagagtttg tgacaaaagt catgtcacaa acttttttct ttgtcatttt
     9721 ttcgtaagaa aaagcaaaat aaaagtatat acattgatta aaagtatata cttggatata
     9781 atgtacttac tatagaatga gaatggtata gagggtggcg aaatatgaaa aaaatgaaaa
     9841 agtataaaat tagaaaatct ggcaatagcg atgtaacaac tatcccacca gaagtaaaag
     9901 aattcatggg tgtgcagaca ggtgacgcaa tcagttatgt ttttcaatct gatggttctg
     9961 tacgaatgat caaagcacaa gaagaacctg atattgattc attagttgat tcgatcatga
    10021 atcaatatga agacgcgtta aaagatttgg ttgatttatg aggtatcttt cgactaaaga
    10081 aattatcaaa atcaacgcga aagttatttt acgtcactcg ccaggcgaaa tgatcggtgt
    10141 aaaagatgca aatgcattag atatggccgt caaacagcct tcacaagcag tattcaatca
    10201 agaactctat cctgaaatct atgaaaaagc ggcgatttta gcaattaatt tagctaaaaa
    10261 acatcctttt cataatggca ataaacgaac tgctcttgtt gccatgttgt tgtttcttca
    10321 attgaataat tgtccagttg ctttttctcg tcaagaagct gttgatttta tcataatgat
    10381 taccactagt tcattggatt tcgattcact aaagtccaag ataaccagtt atttacgaca
    10441 aaccgcaata aaatgaacga attaaaaact ttatttctca attagatcag catgtcatta
    10501 aggaatatgc tgattttttt atgtttaatc atacaaaata gttttttcat aaaaatatgg
    10561 aaaatatgga ttaattttga aataattgat ttaaaaggaa tacaaaagag tgaatagtag
    10621 gaaatagtgg tttagaacta gtgggctga
//
LOCUS       DAZKLM010000091        10272 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3764
            SAMEA115706913-rid22582563.denovo.095, whole genome shotgun
            sequence.
ACCESSION   DAZKLM010000091 DAZKLM010000000
VERSION     DAZKLM010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706913
            Sequence Read Archive: ERR13196647
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10272)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10272)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287823.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287823.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,004
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,849
            CDSs (with protein)               :: 2,849
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 41 of 96
            Pseudo Genes (incomplete)         :: 52 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 15 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10272
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706913-rid22582563.denovo.095"
                     /isolate="VRE3764"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            194..343
                     /locus_tag="ACG842_002254"
     CDS             194..343
                     /locus_tag="ACG842_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HFQ0248772.1"
                     /translation="MNKWRSNPKLNYWGKFIIRTIFYSAVLLFLIYLYHYKNIQGGTF
                     IYNEF"
     gene            354..1868
                     /gene="dltA"
                     /locus_tag="ACG842_002255"
     CDS             354..1868
                     /gene="dltA"
                     /locus_tag="ACG842_002255"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047473 - D-alanine [D-alanyl carrier
                     protein] ligase activity [Evidence IEA];
                     GO_process: GO:0019350 - teichoic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HFQ0248773.1"
                     /translation="MEIKTIIEAIDDWGINEPDRVAYQADETHTFGELKQASDALAYY
                     LEKKVEGDGPIVVFGNLEFEMIVSFLGVVKSGHAYLPIEEHTPKERILSIFRVAKPSM
                     VISIGDWIEDIPSVPVITKKEFQEIKKIPVGFYPRNSVKGTENFYIIFTSGTTGEPKG
                     VQISHDNLVSFVQWTMEDFGIKPGMHFLAQAPFSFDLSVFSIYPALVSGGMLKPLNKA
                     VVQDFRQLFATLPALKLNVWVSTPSFMDICLMEPTFNGENVPELDMFLFCGEELTKKT
                     AESLLDRFPNARIYNTYGPTEATVAISSIQIDQHVLDSYDRLPIGYVKKDTKVSIVQE
                     GKPVSKGETGEIIIAGPSVSKGYLNNPEKTAAAFFCQNDLASYHTGDAGRLDEYGLLF
                     YEGRMDQQVKLHGYRIELGDIEHYLLQDNRIKQAVVVPKYQGTKVQQLVAFVVLENKT
                     KQPDFQLTKSIKEQLLEVVMDYMIPQKFHYVDLLPQTVNGKIDRKKLTAEVNPE"
     gene            1865..3070
                     /gene="dltB"
                     /locus_tag="ACG842_002256"
     CDS             1865..3070
                     /gene="dltB"
                     /locus_tag="ACG842_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0070395 - lipoteichoic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HFQ0248774.1"
                     /translation="MMLPHMIPYAEPFYFFLLAIALVPIVLSLLIWEKRLPVYQSAVT
                     LFFLFVSFGGNYWQQGAALIGYVLWQTILVWGYFVYRQRKNQTGWFFAAVILAIIPLF
                     LTKVTPFFEHGKASLLGFLGISYLTFKSVQMIMEIRDGMIKTYKPFRYIQFLLFFPTI
                     SSGPIDRYRRFEKDQLHPPVRDKYVDLLGKGVQNIFVGFLYKFIIGYYFGQVLLPVVG
                     RIALQHGGISWALVGYMYVYSMYLFFDFAGYSLFAVGTSYMMGYQTPINFNKPFLSWN
                     IKEFWNRWHMTLSFWFRDYVYMRLMFFMMKKKFFKSKIVMSNIGYFALFLLMGIWHGL
                     TWYYIVYGLYHACLICLTDAWIRFKKKHKEQLPSNKFTHGLAVFLTFQAVCFSFLIFS
                     GFLDKLFFS"
     gene            3115..3348
                     /gene="dltC"
                     /locus_tag="ACG842_002257"
     CDS             3115..3348
                     /gene="dltC"
                     /locus_tag="ACG842_002257"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036370 - D-alanyl carrier activity
                     [Evidence IEA];
                     GO_process: GO:0019350 - teichoic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HFQ0248775.1"
                     /translation="MEEQILSILEEITGTDEVRKNRDIDLFDEGLLDSLASVQLLVEL
                     DGELDIQVPVSEFEREDWCTPNKIIQQATALKG"
     gene            3351..4619
                     /gene="dltD"
                     /locus_tag="ACG842_002258"
     CDS             3351..4619
                     /gene="dltD"
                     /locus_tag="ACG842_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070394 - lipoteichoic acid metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HFQ0248776.1"
                     /translation="MKKKLFAIFGPVVLAVGLLFVFFTSSYQVDISNPETIRRASSSM
                     SVNVLKGDAIKNYALSEKQYIPFFGSSELSRISPFHPSVLAEKYQRNYRPFLLGAPGT
                     QSLSQYMMMRSAGDAMKNKKVVFIISPQWFVKNGVKTDYFNTYYSELQTYDWLFSMKK
                     VTPADRYLARRLLTFSKVKENDTLTAILQTIKKGKLPLPESLNQLRSQWNMLKREDEV
                     FSNIGLKDRQQKIDHESKRLPKQYQETELSILANQIGERETTNNPFGLKNDFYTHRIR
                     AHEPELKQSQKNWDYRFSPEFSDFQLVLDQLAKNHNEVLFIIPPVNEKWSDYTGLSQE
                     MLQGFAKKIKFQLNSQGFNRIADFVNQAGTNYFMEDTIHLGWKGWLAADQQIRPFLEE
                     NHITASKYHLDDAFFSKSWQHQIPDKLQLK"
     gene            4760..5842
                     /locus_tag="ACG842_002259"
     CDS             4760..5842
                     /locus_tag="ACG842_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HFQ0248777.1"
                     /translation="MDTKEEQLLIDLTSAKGVPGNEEEVREVFREYAKPFADDIFYDG
                     LGSVIAKHGAGGGPKVFISGHMDEVGFMVTKITEKGFLEFQTLGGWWGQVMLAQQVEI
                     KTREGKIVHGVIGSKPPHVLTPQVRNKPYEIKDMFIDIGASSQEEAKEWGIRPGDMVT
                     PYIEYKRMNGSKYLLAKAWDNRIGTAVSLRVLENLSKEAHPNVLFAGSDVQEEVGLRG
                     ARTSTHLVNPDIAFALDTGTAGDTPGMTPKEADSILGKGPQILIFDASMIPHKKLLNF
                     VIDVAEEMDIPFQYTVISGGGTDAGQMHLTRDGVPSVAITVPVRYLHSHTSVIHEDDY
                     LNTVKLVTELVKRLDSETVEKIRSYE"
     gene            5842..6645
                     /locus_tag="ACG842_002260"
     CDS             5842..6645
                     /locus_tag="ACG842_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HFQ0248778.1"
                     /translation="MNNRIPISEYRKKARVSLENQWGINTWLVFLSIIFTSVIQNFFD
                     GFSPFPEESMQQHLLSFLVDNLLLFAFNYALFYIALVVVRGGRPKSNLLFSVFQRKYF
                     GSILGINLINSVINWLLNSLIFLPMFLISGVSTYMQLLFSSNAVPTGVMPEDFLDISF
                     VLSMIGMIFVSALIMSIVGGLFQFAAWTKFDYPDLSVIQCLKYGWFLLKDRIGTYILL
                     QLSFIGWYLLGAIALFIGLLWVIVYVNVSVAEFYEQARIEKGGPDAYFT"
     gene            6715..7455
                     /locus_tag="ACG842_002261"
     CDS             6715..7455
                     /locus_tag="ACG842_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFQ0248779.1"
                     /translation="MKLTVLGCLGAYPYKNQGTTGYLLQSDGFNLLIDAGSATLIKLQ
                     EHLDPLDLDAVILSHYHHDHIADLGVLQYYWQLHPKRNPETLLSIYGHTKDSIHFEEL
                     TLENVSKGHPYFEAEELKIGPFLITFMETIHPVVCYAMRIVEETTGKVFVFTGDSGYL
                     ESFADFAKDADLFLADTYLFEGNEHHHAHFTSKEAGELAKSANVKKLVLTHLPQFGDL
                     EQLKNEAQKAAGKGIDIELAAVDKVFNI"
     gene            7466..8470
                     /locus_tag="ACG842_002262"
     CDS             7466..8470
                     /locus_tag="ACG842_002262"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017118 - lipoyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HFQ0248780.1"
                     /translation="MIFVPNENHDPRINLAIEQYLLQEMPLDEPILLFYINEPSIIIG
                     RNQNTLEEINRDYVEENGIHVVRRLSGGGAVYHDFGNLNFSFIMPDDGDSFRDFAKVT
                     QPIIQALHELGVAGAELKGRNDLVIDGMKFSGNAMYATNGRMFAHGTIMFDSDINEVV
                     NALKVKKDKIESKGIKSIRSRVTNIKPFLPEEKQKMTTEEFRQEILLKIFGVESVEEV
                     KTYELTDEDWKKINRISEEYYRNWDWNYGKSPDFNFSRQKRFSIGSIEVHLNVSEGMI
                     KEAKIFGDFFGLGDISDVEKKLVGQKYDKASLSKIVSQIDVKKYFGAIEPEELLTLLY
                     "
     gene            complement(8515..>10272)
                     /locus_tag="ACG842_002263"
     CDS             complement(8515..>10272)
                     /locus_tag="ACG842_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0248781.1"
                     /translation="FLTGIAYFFYFIFSPADSNQMKEIKDSWVFYTKDDPDFKFDSKY
                     TRTIPTVDKDETFIMETTLEKPLDEANLLIKGNHQWITVYLGEKILYQRKDYQEESNP
                     GLSLAVIDLPANYIGKKLTLTVSSPYQNYAGLPPKVYLGTTGPLISFIYSQSVPQVLT
                     MIIAVFIAIGTFFYTIYLMYKQKRLEYSLILLSCFALALGFEAVSEDILSGILFEPFV
                     HSFLSHLFIILTSAFIICYYWSRMFYYKKWYGIFCTFQLLIFIGVLLYATFTPAELPE
                     LMPIANIASVISTLVTSLACIGEAYKNNRFYVACTPWIVLVAIIHCLIYIQTALGIYQ
                     TTINWSTILYIIILIVIISYNLIDHILNTDKDRRQVDFLKIKNDLLEQHYEQLRQHIQ
                     EVNTLRLEFVQEMENLQDLMHTDQTKAKNYVEKILEEAKNFEMLCSFCNHQVTNLILA
                     RYQKLAAKRNIQITFQADLPEELPINDDDLAQLLIHALEHSFRETHAIENPLYRKIYL
                     SIHEQEEHFVICCEHSAHYDTNLFSRGITEELDDQERFDLMMMKDVADRYTGTLTQEK
                     DTFIDRITVQLSRNYPNPV"
BASE COUNT         3296 a         1699 c         2081 g         3196 t
ORIGIN      
        1 aacctcttta ttttttatat tttttaaaag tacgagaaat tataaagaaa aaagcagtga
       61 tttgatgtgc tatttttata aaagctatgt gacaaaactg ttagtaaaaa aacggtttct
      121 gtaagctaga cacatgtttc tttttgtcaa aattagttac actttgtgtg aggttatatg
      181 aggagggaat ccaatgaaca aatggcgttc taatccaaaa ttgaattatt ggggaaaatt
      241 catcattcga acaatttttt atagtgcagt actgctgttc ctgatttatc tgtatcacta
      301 taaaaatatt caaggaggaa cttttatcta taacgaattt taggaggaag agtatggaaa
      361 tcaaaacgat tattgaagca atcgatgatt ggggaataaa tgaacctgat cgagttgctt
      421 atcaggcgga tgagacgcat acttttggtg aattgaaaca agcatcagac gcacttgctt
      481 attatttaga aaaaaaggta gaaggcgatg gaccaattgt cgtttttggc aatttggagt
      541 ttgaaatgat tgtctcattt ctaggggttg tcaaatctgg ccatgcctat ttgccgattg
      601 aagagcatac acccaaggag cgtatcctat cgatctttcg ggtggctaaa ccatctatgg
      661 tcatcagtat cggggactgg atagaagata ttccttcggt tcctgtcata acaaaaaaag
      721 aatttcaaga aatcaagaag ataccagtag gattttatcc cagaaatagc gtaaaaggta
      781 cagaaaattt ttatattatt tttacttctg gtactactgg agaaccaaaa ggcgtacaaa
      841 tcagtcatga taatctagtc agctttgttc aatggacgat ggaagacttt gggatcaagc
      901 ctggtatgca tttcttagcc caagcgccat tttcttttga tttatcggta ttttctattt
      961 atcctgcatt agtttctggc ggaatgctga agccgttaaa taaagcagtt gttcaagact
     1021 ttcgtcaatt attcgcgaca ttgccagcct tgaagttgaa tgtgtgggtt tctactccat
     1081 catttatgga tatttgcttg atggaaccta cattcaatgg agaaaatgtg ccggaattgg
     1141 acatgttttt attttgcgga gaagaattga cgaaaaaaac tgcagaaagt ctacttgatc
     1201 gattcccaaa tgcacggata tataatactt acggtccaac agaagcaact gtagctattt
     1261 caagtattca gattgaccag catgtactag atagttatga ccgactaccg attggctacg
     1321 taaaaaaaga cacgaaagtt tctattgtcc aagaaggtaa acctgtttca aaaggagaaa
     1381 cgggtgaaat cattattgca ggaccaagcg tatcaaaagg ttacctgaac aatcccgaaa
     1441 agacagctgc tgcgttcttt tgtcaaaatg acttagcaag ttaccataca ggggatgcag
     1501 gtagactaga tgaatatggg cttttatttt atgaaggacg tatggatcag caggtcaaat
     1561 tacatggata ccggattgaa ctcggggata tcgaacatta tctgctccaa gacaaccgaa
     1621 tcaaacaagc tgtcgtcgta ccaaaatacc aaggaacaaa agtacagcag ctggttgctt
     1681 ttgtcgtatt agaaaacaaa acgaaacagc cggattttca gttgactaaa tctatcaaag
     1741 aacaattact agaagttgta atggattata tgattcctca gaagttccat tatgtcgatc
     1801 tgctgcctca gacagttaat ggaaaaatcg atcgaaaaaa attaacagct gaggtgaatc
     1861 ctgaatgatg ttacctcata tgatccctta tgcagagccg ttttattttt ttcttttagc
     1921 cattgcgctt gtaccgatcg ttctatcttt attgatatgg gaaaaaagat taccagttta
     1981 tcaatcagca gtgacacttt tctttttatt tgtcagcttt ggaggtaatt attggcaaca
     2041 gggagcggcg ctgattgggt atgttttgtg gcagacgata cttgtatggg gctattttgt
     2101 ttatcggcaa aggaaaaatc agacaggatg gttttttgcc gccgttattt tagcgataat
     2161 tccactgttt ttaacgaaag tgactccttt ttttgaacat ggaaaagcat cgttacttgg
     2221 ttttttagga atctcttatt taacctttaa atctgtacaa atgatcatgg aaataagaga
     2281 tggcatgatc aaaacatata agccatttcg ttatatccag tttctgctgt tctttccaac
     2341 aatctcttct ggaccaatcg atcgctatag gagatttgaa aaagatcagc tccatccgcc
     2401 tgttcgtgat aaatatgttg atcttttggg aaaaggcgtc caaaatattt ttgtcggttt
     2461 tttatataaa ttcattatcg gttattattt tggtcaagta ctgcttcctg tagtgggacg
     2521 cattgcctta cagcatggtg gtatttcatg ggcattagta ggttatatgt acgtgtacag
     2581 tatgtatttg ttttttgatt ttgctggcta tagcttattt gcagtcggta caagctacat
     2641 gatgggatat cagacaccaa tcaatttcaa taaacctttt ttatcatgga atatcaaaga
     2701 attctggaat cgttggcata tgacgctgtc cttttggttc cgtgattatg tatatatgcg
     2761 attgatgttc ttcatgatga agaaaaaatt ttttaaaagc aagatcgtaa tgtcaaatat
     2821 cggttatttt gctttgtttc ttctaatggg tatatggcat ggattgacgt ggtactacat
     2881 cgtatatgga ctgtatcatg cttgtctgat ctgtctgaca gatgcatgga tccgatttaa
     2941 gaagaagcat aaagagcagt tgccttctaa caaatttacg catggcttag ccgtattttt
     3001 gacatttcaa gcagtatgtt ttagcttcct gattttttca ggatttttag ataaactttt
     3061 ttttagctaa cgaatagtga catattaatt tatataacaa aggagaaaag gaaaatggaa
     3121 gaacaaattt tatcaatctt agaagaaata acaggaacag atgaagtaag aaaaaatcgt
     3181 gatattgatc tatttgatga agggttgctt gattctttag catctgttca attattagtc
     3241 gagctagacg gagagctgga tatccaagtc cctgtatctg aattcgagcg agaggattgg
     3301 tgcacaccga ataaaatcat tcaacaagca actgcattga agggttgagt atgaaaaaga
     3361 aactttttgc tattttcggc ccggttgttt tagctgttgg cttactgttt gtgtttttta
     3421 cctcctccta tcaagtagat atctctaatc cagaaacgat tagacgtgcc tcttcttcca
     3481 tgtcagtgaa cgtgttgaaa ggagatgcga taaaaaatta tgcattatct gaaaaacaat
     3541 atattccttt ttttggctca agtgaattaa gcagaatcag tccatttcat ccgtctgttc
     3601 tggcagaaaa ataccagcga aactatcgtc catttttgct gggagcaccc gggacacaat
     3661 cgttaagcca atatatgatg atgcgttctg caggagatgc tatgaaaaac aagaaagtcg
     3721 tctttattat ttctcctcag tggttcgtta aaaatggtgt aaaaactgat tactttaata
     3781 cgtattattc tgaacttcaa acatacgact ggttgttttc aatgaagaaa gtaacgcctg
     3841 ctgatcgcta tttagctaga agattattaa ctttctcaaa agtaaaagag aatgatacgt
     3901 tgacggcgat tctgcaaacg atcaaaaaag ggaagctacc tttacctgaa tctttgaatc
     3961 agctgcgcag ccaatggaac atgctgaaga gagaggatga agtattcagc aatattgggt
     4021 tgaaagatcg acagcaaaaa atcgatcatg agtcaaaacg attaccaaaa caataccaag
     4081 aaacagaatt atccatttta gcaaatcaaa ttggagaacg agaaacgacg aataatccat
     4141 tcgggttgaa aaacgatttt tatacgcatc gtatccgagc ccacgagcca gagctcaaac
     4201 agtcacaaaa aaattgggat tatcgttttt cgccagaatt ttctgatttt cagctggtct
     4261 tggatcaatt agcaaaaaac cataacgaag tattgttcat catcccaccg gttaacgaga
     4321 aatggagcga ttataccgga ctatctcaag aaatgttaca aggatttgcc aaaaagatca
     4381 aatttcaatt aaatagccaa ggattcaatc gaatagctga ttttgtcaat caagcgggta
     4441 ccaactattt tatggaagac acgatccatc taggctggaa aggctggttg gctgcagatc
     4501 aacagatcag accgttttta gaagaaaatc atattactgc tagtaagtat catcttgacg
     4561 atgctttttt tagcaaatct tggcaacatc aaataccaga taaactacaa ttaaaataaa
     4621 gaaaacaggc gagtgttgct cgcttgtttt ctttattttt gaatattctg aataaaagat
     4681 aaaaatacca agtttgcctg aacaaaaatc ataagcaatg gtataatcac aatatgaata
     4741 tagaaatggg ggaaaacatt tggatacaaa agaagaacag ctacttattg atttaacttc
     4801 tgcaaaagga gtacctggaa atgaagaaga ggtaagagaa gtattcagag aatatgccaa
     4861 accattcgct gatgatattt tttatgacgg tttaggcagt gtgattgcca aacatggtgc
     4921 tggcggaggc cccaaggtct ttatttctgg acatatggac gaagttggtt ttatggtaac
     4981 aaaaataacc gaaaaaggat tcttggaatt ccaaactctt ggaggttggt gggggcaagt
     5041 catgcttgct cagcaagttg aaatcaaaac acgtgaaggg aaaatcgtcc acggtgtgat
     5101 tggttccaaa ccaccacatg ttttaacacc tcaagtaaga aataaacctt atgaaataaa
     5161 ggatatgttc attgatattg gcgcatcaag ccaagaagaa gccaaagagt ggggcatacg
     5221 tcctggagat atggtcactc catatattga atacaaacga atgaatggca gtaaatattt
     5281 actggcaaaa gcatgggata atcgtatcgg gacagctgtt tctctgcgag tattagagaa
     5341 tctatctaaa gaagcacacc caaatgtttt attcgcagga agcgatgtgc aagaagaagt
     5401 aggcttgcga ggtgctcgaa caagtacaca ccttgtaaat cccgatattg cttttgcttt
     5461 agatactggt acagcaggag acactccagg aatgacacca aaagaagcag attcgatttt
     5521 aggaaaaggc ccacagattt tgatttttga tgcatctatg atcccgcata aaaaattatt
     5581 gaattttgtt attgatgtag cagaggaaat ggatattcca ttccaatata cagtgatttc
     5641 tggcggagga actgatgcag gacaaatgca tttgacaaga gatggtgttc cgtctgtagc
     5701 cattaccgtt cctgttcgtt atctgcattc ccatacatca gtgattcatg aagatgatta
     5761 tttgaataca gtgaaactag tcacagaatt agtgaaacgg cttgatagtg aaacagttga
     5821 aaaaattcgg agttatgaat aatgaataat agaataccta tttctgaata tcggaaaaaa
     5881 gcacgagttt ctttggaaaa tcaatgggga atcaatactt ggcttgtttt cttatctata
     5941 atatttacga gcgtcatcca gaactttttt gacggtttca gtccttttcc ggaagaaagc
     6001 atgcagcagc acttattgag ttttctagtc gataatttac ttttattcgc attcaactat
     6061 gcattgtttt acattgcgct agtagttgta cggggtggca gaccaaaaag taacctttta
     6121 ttttcagttt tccaaagaaa gtattttggt tcgatacttg gcatcaattt gatcaattca
     6181 gtcatcaact ggctactcaa cagtttaatc tttctgccca tgtttttaat cagcggagtg
     6241 agtacttata tgcagctgct tttcagttca aatgcggtac ctacgggtgt catgccagaa
     6301 gattttcttg atatcagctt tgttttatcg atgataggga tgatttttgt ttcagcactc
     6361 atcatgagta ttgttggcgg tttattccaa tttgctgcat ggacaaagtt cgactatcct
     6421 gatttgtcgg ttattcaatg tttgaaatat ggctggtttt tattgaaaga tagaattggt
     6481 acgtatattt tgttgcagct gtcttttatc ggctggtatt tgctaggagc aatcgctttg
     6541 ttcatcggtc ttttgtgggt cattgtttat gtgaatgtgt cagttgcaga attttatgaa
     6601 caagcacgga ttgaaaaagg aggtccagac gcctatttta cttagcatgt aagcagttta
     6661 ttcatcaaaa aaataacttt tgtggtagga taaatctttg agggggaatg tacgatgaaa
     6721 ttaactgttt taggatgctt aggagcgtat ccttataaaa atcaagggac aacaggttat
     6781 ttgcttcaat ctgacgggtt caatttgttg atcgatgcag gtagtgctac gctgatcaaa
     6841 ttacaagaac accttgatcc acttgatcta gatgctgtga tccttagcca ttatcatcat
     6901 gatcatattg ctgaccttgg tgtattacag tattactggc aacttcatcc taagagaaat
     6961 ccggagacat tgctttcaat ttatggacac acaaaagatt ccattcactt tgaagaattg
     7021 acgttggaaa atgtctcaaa aggacatcct tattttgaag ctgaagaatt aaaaattggt
     7081 ccttttctga ttacttttat ggaaacaatc catccagtag tatgttacgc catgcgtatc
     7141 gtagaagaaa caacggggaa agtattcgta tttacaggtg attcaggtta tttggaaagt
     7201 ttcgctgatt ttgcgaaaga tgctgatttg tttctagcag atacctatct ttttgaagga
     7261 aatgaacatc atcatgccca tttcacttca aaagaagctg gagaacttgc gaaatcagct
     7321 aacgtaaaga aacttgtact tacacaccta cctcagtttg gtgatttaga acaattgaaa
     7381 aatgaagcgc aaaaggcagc aggcaaaggg attgatatag aacttgctgc agttgacaaa
     7441 gtgtttaaca tttaggagga agaacatgat ttttgtacca aatgaaaacc acgacccaag
     7501 aatcaattta gcaattgaac aatatttgct gcaagaaatg cctttagatg aacctatttt
     7561 gctcttttat atcaatgaac cttcaatcat catcggcaga aaccaaaata cgcttgaaga
     7621 aatcaatcga gattatgtgg aagaaaatgg cattcatgtt gtccgtcgct taagtggagg
     7681 cggagctgtc taccatgatt ttggaaacct gaactttagt tttatcatgc ctgatgatgg
     7741 ggattcattc cgtgactttg cgaaagtaac acagccaatc atccaagcac ttcatgaatt
     7801 gggtgttgct ggggcggaat tgaaaggccg aaatgatcta gtcattgatg gcatgaaatt
     7861 ttctggcaac gcgatgtatg ctaccaacgg tcggatgttt gctcatggaa caattatgtt
     7921 cgactctgat atcaatgaag tagtcaatgc attaaaagta aaaaaagaca aaatcgaatc
     7981 aaaaggaatc aaatcgatcc gttctcgtgt aacgaatatc aaaccatttc ttccagaaga
     8041 aaagcaaaaa atgacaacag aagaatttcg tcaagaaatc ttgttgaaaa ttttcggcgt
     8101 agagtcagta gaagaagtaa aaacgtacga attgactgat gaagattgga aaaaaatcaa
     8161 tcgaatctct gaggaatatt atcgaaattg ggattggaat tacgggaagt ctccagattt
     8221 caatttctct cgccaaaaac gtttttctat cggttcgatt gaagtccatt tgaatgtctc
     8281 agaaggaatg atcaaagaag caaaaatctt tggcgatttc ttcggactag gagatatctc
     8341 tgacgtggaa aaaaaattgg ttggacagaa atatgataaa gcatctctat caaaaatagt
     8401 cagccaaatt gatgtgaaga aatattttgg tgcgatagaa ccagaagaat tgcttacctt
     8461 actttattga aaataaaaaa tagcggaatc cacatatatg gattccgcta ttttttatac
     8521 aggattcggg taatttcgag agagttgaac agtgatccga tcaatgaaag tatctttttc
     8581 ctgcgttagt gttcctgtgt aacgatccgc tacgtctttc atcatcatta agtcaaaacg
     8641 ttcctgatca tctaattctt ctgtgatgcc tcgactaaat aggtttgtat cgtaatgtgc
     8701 actatgttcg caacaaatga cgaaatgttc ctcttgttcg tgtatcgaca aataaatttt
     8761 tcgatacagt ggattttcga tggcatgagt ttctctaaag gaatgctcta aagcgtggat
     8821 caaaagttga gccagatcgt catcgttgat aggaagttct tcgggaagat ctgcttggaa
     8881 ggttatctga atattccgtt tggcagctaa cttctggtaa cgcgctaaga taagatttgt
     8941 cacctggtgg ttgcaaaaag aacacagcat ttcgaaattt tttgcttctt ctaatatctt
     9001 ttccacatag tttttagctt ttgtttggtc cgtatgcatt aaatcttgca gattctccat
     9061 ctcttggaca aattccaggc gaagtgtatt tacttcttgg atatgctgtc gaagctgctc
     9121 ataatgttgc tccaacaaat catttttgat ttttaagaaa tctacttgtc tccgatcttt
     9181 gtcggtattt aagatgtgat cgattagatt gtagctgata ataacgatta gaatgataat
     9241 atataaaatc gtagaccaat tgatcgttgt ttgatagata cctaatgccg tttggatgta
     9301 aatcaagcaa tggataatgg ctaccaagac gatccaaggt gtacaagcta cataaaaacg
     9361 gttattctta tatgcttcgc caatgcatgc aagtgaagtg actaaagtgc ttataacact
     9421 agcaatatta gcaatcggca ttaattcagg aagttctgca ggagtaaaag ttgcataaag
     9481 cagaacgccg ataaaaatca aaagttggaa cgtacaaaaa attccgtacc attttttata
     9541 gtaaaacatt cggctccaat agtagcagat gatgaaagct gacgttaaga taataaataa
     9601 atgagataaa aaagaatgaa cgaatggttc aaaaagaata ccacttaaaa tatcttctga
     9661 tacagcttca aatcctaatg ccaaagcaaa gcaacttaag agaataagtg agtattcaag
     9721 acgtttttgt ttatacatca agtagatcgt atagaaaaag gtccctatag cgataaaaac
     9781 agcgattatc atcgtcaata cttgcggcac tgattgtgaa taaataaaac taatcaatgg
     9841 tccagttgtt cctagataaa ctttcggtgg cagtccagca tagttctgat aaggagatga
     9901 gactgtaagg gtcaattttt ttccgatata atttgctggt aaatctatca cagccaatga
     9961 gagtccggga ttgctttctt cctgataatc tttccgttgg tataaaatct tttcgcctag
    10021 atatacagta atccattgat ggtttccttt gattagtaaa tttgcttcgt ccaacggttt
    10081 ttcaagtgtc gtttccataa tgaatgtttc gtctttatcc acggtaggaa tcgtacgagt
    10141 gtacttgctg tcaaacttaa aatcaggatc atcttttgta tagaaaaccc acgaatcttt
    10201 tatttctttc atttgattgc tgtccgcagg agaaaaaata aaataaaaga aataagcaat
    10261 tcctgttaaa aa
//
LOCUS       DAZKLM010000092        10246 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3764
            SAMEA115706913-rid22582563.denovo.096, whole genome shotgun
            sequence.
ACCESSION   DAZKLM010000092 DAZKLM010000000
VERSION     DAZKLM010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706913
            Sequence Read Archive: ERR13196647
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10246)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10246)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287823.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287823.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,004
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,849
            CDSs (with protein)               :: 2,849
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 41 of 96
            Pseudo Genes (incomplete)         :: 52 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 15 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10246
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706913-rid22582563.denovo.096"
                     /isolate="VRE3764"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            74..547
                     /locus_tag="ACG842_002264"
     CDS             74..547
                     /locus_tag="ACG842_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HFQ0248782.1"
                     /translation="MKFKTLLLKFVVLGISAVFILFGVLFYTQIATSEKGFNFDWQTT
                     LFVSVIYFALLLGIVIAYFLLKLLKKIEDNQAFSTQSLNILKKIERTILFIGFAFFGL
                     LPKFMKVADLDDAPGLMLVGIAVVFIPFAVYTLTSVLEKLLEHAILLKTDHDLTV"
     gene            556..783
                     /locus_tag="ACG842_002265"
     CDS             556..783
                     /locus_tag="ACG842_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0248783.1"
                     /translation="MAIIVNLDVVLAQKKMSVTELSEKVGITMANISILKNGKAKAIR
                     FSTLEKICQVLECQPGDLLTYKNDSNTIPEE"
     gene            1419..1790
                     /locus_tag="ACG842_002266"
     CDS             1419..1790
                     /locus_tag="ACG842_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0248784.1"
                     /translation="MPFIVYFFLSLLVIYIPLPTFFLLVNRLAEQHDTIAALTKLCLL
                     LFVCLDYFTSYYWLKQTRVKKRYYFFLVFFNLFEFLIHCFLLHQYHTIHLWIIWVFKV
                     VVLFCMFTFPISRKFREALFY"
     gene            complement(2046..2288)
                     /locus_tag="ACG842_002267"
     CDS             complement(2046..2288)
                     /locus_tag="ACG842_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFQ0248785.1"
                     /translation="MGFIWSLIVGGIIGAIAGAITNKGSSMGIIANVVAGLVGSALGQ
                     ALLGSWGPSIAGMAIVPSLIGAIILVLIVSFFARKL"
     gene            complement(2320..3222)
                     /locus_tag="ACG842_002268"
     CDS             complement(2320..3222)
                     /locus_tag="ACG842_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HFQ0248786.1"
                     /translation="MNNNNKNNNNKMNNQKPVSAHPDPTTPNQSNGVNDEKNKTAPVS
                     AHPDPVVPNVQQGVEEKKHDAEKHAPHHPGTPESKVPEQHDAIDPSKPVEEQKHPSDH
                     AKAQEKLSSDHTNGQGKLGSDHTNNQGKHDSEQEDSMRGELNYEDKVVQKIIGIAIEQ
                     VDGLLSANGGFFSNVAGKLVNTDNVTAGIETEVGKKQVAVDMDVIVEYGKDIEKIFEE
                     MQEVIGKEVKNMTHLEVIEVNANVVDIKTKEEFEKDQETVQDKVTDAAKKTGEFASNQ
                     TDKVKSAAGSGAQKVKENTEPRVQ"
     gene            complement(3235..3423)
                     /locus_tag="ACG842_002269"
     CDS             complement(3235..3423)
                     /locus_tag="ACG842_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HFQ0248787.1"
                     /translation="MKELFSTYKLPIIFGVLGLILAVLWITVGFFKTLLILIMAAIGV
                     AIGFYLKQTKILDHYFKS"
     gene            complement(3437..4000)
                     /gene="amaP"
                     /locus_tag="ACG842_002270"
     CDS             complement(3437..4000)
                     /gene="amaP"
                     /locus_tag="ACG842_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HFQ0248788.1"
                     /translation="MNKGVKTIGVIGTLLLLSVLLFAILLSATYPLPFQLEKFRFFSI
                     TNSYVQQYVFWLAVAFAILTIITLLVFLFYPKTIGNFLLKHGDGELTLDKKAIEGMVR
                     SHLHEDEFIHSPKVNVKATKNKIAVNVKGELKRTSALIGKTGTLMKEIENEVQHILGT
                     KEPVKVSVKYTGFEEPAKRSNDRKRVE"
     gene            complement(4038..4931)
                     /locus_tag="ACG842_002271"
     CDS             complement(4038..4931)
                     /locus_tag="ACG842_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFQ0248789.1"
                     /translation="MTEPKLIDPRNLYHTEKFPKQDQDTPALQQNMIPVPDCGEESYE
                     GNNQLENRRVLITGGDSGIGRAAAIAFAREGADIALQFFPGEEKDAEEVAKYVKVAGR
                     KIVLLPFDLRDEQAPKEIIDKAVSELGGLDTMVLNAGQQISCQSIAELPLEQVKDTFM
                     INIIAMFALVKEAVPHIPAGGAIVTTTSVQAFNPSEHLLDYAATKASIANFTIGLAKQ
                     LAPKGIRVNGVAPGPIWTPLQLDEGQPSDSIPEFGQQSLLERAGQPGELAPVYVFLAS
                     NKASYVTAQIYGVTGGEAINL"
     gene            complement(5009..5932)
                     /locus_tag="ACG842_002272"
     CDS             complement(5009..5932)
                     /locus_tag="ACG842_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HFQ0248790.1"
                     /translation="MDEEKNVIETDEKKYNWLVLDSAKPEEIEQAIEEFELPDDIFVG
                     TEYPEEVSRMERLYETKLEHPFSLVVINLDQSDNRIEKKLTPISFVLSDNLLITCIDR
                     KTEFVDRLLTKHGNHLNSFEKIITYTLLDIYTHYVKELREMKKRIDALDKAARKTTEN
                     EELYKQADLERDIVYIDHTLRDQRETMDQLWNSETFIKRLDDEQLLYDVHLRQRQTEK
                     MIEIYRDLLESIGDLFNGMMDNNLNHIMKYLDSAALIISVPALFAGIWGMNTGGLPGK
                     SSALGFILVVFGSLIAAVVLGYHLYRKDYTN"
     gene            complement(5981..6331)
                     /locus_tag="ACG842_002273"
     CDS             complement(5981..6331)
                     /locus_tag="ACG842_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HFQ0248791.1"
                     /translation="MEITMSKNAVETLIEKVGENTKIALALINDSDPFLRDKGAFAKG
                     SFFQIIPFVSEFGEYATKIEHPLLDIYTSKLEQNYFGKRLNMDFNKQLDSFSLENEIA
                     VLDYNIKLKNCFFS"
     gene            complement(6364..7266)
                     /locus_tag="ACG842_002274"
     CDS             complement(6364..7266)
                     /locus_tag="ACG842_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HFQ0248792.1"
                     /translation="MNKKEIAVVASCNTKFVPHLAALFVSVLDNCNPSKFVRFYVIDD
                     DIDFESKQLLRFSVKNARMNSDVEFLKINKEFFTNVVISDRIPETAYYRIAIPELFRG
                     TEVERILYMDCDMIALQDISKLWRLDFGDSIVAAVEDAGFHQRLEKMKISAKSMRYFN
                     SGLMLINVKKWLDENITQKVLDFIEHNPEKLRFHDQDALNAILHDRWLPLHPRWNAQG
                     YIMAKAKKHPTAAGEREYEETRNNPYIIHFSGHVKPWSKDFEGPTKKYYEKYAGMTAF
                     RCVAKFPKYPKYAKVQPKTDVVVK"
     gene            complement(7259..8116)
                     /locus_tag="ACG842_002275"
     CDS             complement(7259..8116)
                     /locus_tag="ACG842_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HFQ0248793.1"
                     /translation="MEKRYGVVPVVTASDENYAPYLSVMIATALENCNKTRRIKFYVI
                     DDGLSEYSKEGLEETVNKYSSNASIQFLTVEKDIYEDFLVSDHITTTAYLRISLPNLL
                     AKEDYKKVLYLDSDVLVLDDIVKLYDEPLNGKTIGAIIDPGQVKALERLGIDSDDLYF
                     NSGVMVIDIDQWNKKEITEKTIHYLSENGDRIIYHDQDALNAVLYEDWEQLHPKWNMQ
                     TSLIFERHPAPNEKYERQYKEGNEKPSIVHFTGHDKPWNTLKDHPYTNLYLKKLAHST
                     LTKVGEINE"
     gene            8450..9259
                     /locus_tag="ACG842_002276"
     CDS             8450..9259
                     /locus_tag="ACG842_002276"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFQ0248794.1"
                     /translation="MKLAAIDLDGTLLDRNGKIPEENIQALRKFDENGGIVTVATGRN
                     SISAKDIFSELGIGGYLISSNGSLIAEMKDGKMAKVLRRSKIDSDTLRKAFFYAQEAK
                     ISIIASRETQDDQLTFDESNLVKDDPYYEHFNLQNHSFDQILNMLEDPSLSYLKLALT
                     DKSEEKLQKLQRDLQADGIQSVFSDPHFLEITPTGITKAHSLVFLADYLGIESSDIMA
                     FGDQENDIAMLNYSGLSIAMGNAQRQVKEIANQVTLTNEEAGVAYILKNFY"
     gene            9299..9796
                     /locus_tag="ACG842_002277"
     CDS             9299..9796
                     /locus_tag="ACG842_002277"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family cysteine hydrolase"
                     /protein_id="HFQ0248795.1"
                     /translation="MLVIIDMQNHILDPNSEFYIPESDQLAERIAQRLAKARQNNEYI
                     LFTRDIPVELKDKEEEREDLQLIPLLSPQENERVIKKYYFTIPPETLAEIQETLFESK
                     EEQKEIEVVGIETNLCVLSNLIGLQSAFPEADFFVDPMLVSSRKHGESALELLKDFNV
                     SIVES"
     gene            complement(10184..10246)
                     /locus_tag="ACG842_002278"
     tRNA            complement(10184..10246)
                     /locus_tag="ACG842_002278"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10218..10220),aa:Ile,seq:cat)
BASE COUNT         2961 a         1941 c         1764 g         3580 t
ORIGIN      
        1 tcgataaaaa atagttgttt tacaataatt attctgctat attttagcta tcaaagataa
       61 aagagggata aaaatgaaat ttaaaacatt gctattaaaa tttgtcgttt taggtataag
      121 tgccgtattt attttgttcg gcgtactttt ttatactcag atcgctacaa gcgaaaaagg
      181 attcaatttt gattggcaga ccacattatt tgtttctgtc atttactttg cccttctcct
      241 agggatcgtt attgcttatt tcttattgaa actattgaaa aaaatcgaag ataaccaagc
      301 tttttccact caaagcttaa acatactaaa aaaaatcgaa cgaaccattt tgtttattgg
      361 tttcgccttt tttggcttac ttcctaaatt tatgaaagta gcggatttag acgatgctcc
      421 aggtttgatg ttggtaggaa tcgctgtcgt attcattcct tttgcagtat acacattaac
      481 aagcgtgctg gaaaaactgc ttgaacatgc gattttactt aagacagatc atgatttgac
      541 agtgtgaggt gcgacatggc aattatcgtt aatttagatg tagtattagc tcaaaaaaaa
      601 atgagtgtga ctgaattgag tgagaaagtg ggcattacga tggcaaacat ctctattcta
      661 aaaaatggaa aagcaaaagc catccgtttt tccactctcg aaaaaatctg ccaagttttg
      721 gagtgtcaac caggtgattt gttgacctat aaaaatgact cgaatactat accagaagaa
      781 taaaataaag tagaacaaac agtaaataaa aaataatctg aatgatagat gtgtgagact
      841 tttagaagtt atttacgtct attattcaga ttttttattt cgattgaata ccgttttatc
      901 tataaataag atatgtactg actcatacaa agggggcctt ttcttctcca tgtactatca
      961 cttttcctga tacaactggt ttcctgttac gttgtagttg gatctgcttt tagtataata
     1021 ctttattcat tccaccagac tgatagcagt ttgagcagtc taaataatca tgtagtatta
     1081 caggccattc tgtttcatac cttgaagtgc ttaaaacttc agctaatggc ccattgtgtt
     1141 aactcttact ccattctttt ttagtaagct cccatatata ctgtaaagcc ttctatatat
     1201 tatgtcatgt tgctttttaa catttactat gtagttttga taaaatgatt tgaataacag
     1261 tgatggacaa ttctttttta tttttaacat tattttgata ataatgatta tttatttaaa
     1321 aatacattag ataacatttg ttacgacacg aaaaagcttc acaaacaaat atcgttattt
     1381 taaaatgagg gcgaactcac acaattagga ggaatagtat gccatttatt gtttactttt
     1441 ttctttcatt actcgtgatc tatattccat taccaacatt tttcctgctc gtcaatcgtc
     1501 tagcagagca acatgatacg attgctgctc ttacgaaact ttgtttactt ctatttgttt
     1561 gtctagatta ctttacgagc tattattggc taaaacagac aagagtaaaa aagagatatt
     1621 atttcttcct tgtctttttc aatctctttg agtttctgat ccattgcttt ttacttcatc
     1681 agtatcatac gatccatttg tggatcatct gggttttcaa agtggtcgta ttattttgta
     1741 tgttcacctt tcctatctca agaaaattta gggaggcatt gttttattaa agaagcttct
     1801 ctgtatcaat aaagacacat ttaaggaaaa acttttaaaa atctaattgg gaatttgccc
     1861 gcataaataa gaggctgtga caaaagtctt gtcacagcct cttattccgt ataaacagtg
     1921 ttcaaaagct tgcatcttcg ataatatgcc caaccaaatg aaaaatatga gaagctattt
     1981 ttgtttggct gttcttatta cttgatgaag aacagctttt tcacgacctc tttttaaaag
     2041 tctttttaca gttttcgagc aaagaatgat acgattagta ctaaaatgat ggcaccaatc
     2101 aatgatggca cgattgccat tcctgcaata cttggtcccc aagaacctaa taatgcttga
     2161 ccaagtgctg atccaactaa acctgctacg acatttgcaa taatccccat cgaagaacct
     2221 ttattcgtga ttgctcctgc aattgctccg ataattcctc cgacgataag tgaccaaata
     2281 aatcccatgt ttcgttcctc ctaatgtttt acttccttat tactgaaccc gtggttctgt
     2341 attttctttt actttttgtg cgccgcttcc agctgctgat tttactttat ctgtttgatt
     2401 cgaagcaaat tcaccggttt ttttcgctgc gtcagttact ttgtcttgca ccgtttcttg
     2461 atctttttca aattcttctt ttgttttgat gtccactacg ttagcgttga cttcgatcac
     2521 ttctaaatgt gtcatatttt ttacttcttt gccgattact tcttgcattt cttcaaagat
     2581 tttttcgata tctttgccat attccacaat gacatccata tcaactgcta cttgtttttt
     2641 accgacttct gtttcgatcc cagccgttac attatctgta ttcacaagtt ttcctgccac
     2701 attagagaag aaaccaccgt ttgcagataa taaaccgtct acttgttcaa ttgcgattcc
     2761 gatgattttt tgcacaactt tatcttcata attcagctca ccgcgcattg aatcttcctg
     2821 ttcagaatca tgctttcctt ggttatttgt atgatcagaa ccaagttttc cttgtccgtt
     2881 tgtgtggtca gaactaagtt tttcttgtgc tttcgcatgg tcagaaggat gtttttgctc
     2941 ttccacaggt ttagaaggat cgattgcatc atgttgttct ggaactttag attctggagt
     3001 tcctggatga tgaggtgcat gcttttctgc atcatgtttc ttctcttcta ctccttgttg
     3061 aacgttaggg actactggat ctggatgtgc agatactggt gctgttttgt tcttttcatc
     3121 gttcacacca tttgattggt ttggtgtcgt tgggtctgga tgtgcagaca ctggtttttg
     3181 gttgttcatt ttattgttat tatttttgtt attgttattc atggatgttt cctcctaaga
     3241 tttaaaatag tgatctaaga ttttcgtttg tttcagataa aaaccgatag cgacgccgat
     3301 cgctgccatg atcaagatca ataaggtctt gaaaaagccc acggtgatcc ataaaacggc
     3361 taatatcaat ccaagtaccc caaagatgat agggagcttg tatgtcgaaa atagttcttt
     3421 catttttggt ccccccctat tctactcgtt tgcggtcatt gcttcgttta gctggttctt
     3481 caaagcctgt gtatttcacc gatactttca ctggctcttt tgttcccaat atgtgttgta
     3541 cttcattttc gatttctttc atcaatgttc ctgttttacc aatgagtgct gaagttcgtt
     3601 tcaactcccc ttttacattg acggcgatct tgtttttcgt tgctttgaca tttacttttg
     3661 gtgaatggat gaattcatct tcatgcaagt gagaacgaac cattccttca attgcttttt
     3721 tatccaacgt caactcccca tcaccatgtt tcagtagaaa gtttccgatt gtttttggat
     3781 aaaacaggaa aacaagtaac gtgataattg tcaaaatagc aaatgcaacg gcaagccaaa
     3841 atacatattg ttgcacgtat gaattagtga tggaaaagaa tcgaaacttc tctagctgaa
     3901 aaggcaatgg ataggttgca cttaatagta tcgcaaacaa aagcacagat agcaaaagta
     3961 atgttccgat cactccgatc gtttttacac ctttattcat tttcgcacct cctcataggg
     4021 gtgaaacgat caacttctta taagttgatc gcttctccac cagttactcc gtaaatttgg
     4081 gctgtaacat aacttgcttt attcgatgct aagaaaacat aaactggtgc taattctcct
     4141 ggctgacctg cacgttctag taaactttgc tgaccaaatt cagggatcga atcagaaggt
     4201 tgtccttcgt ctagttgtaa tggtgtccag attggaccag gtgctacacc atttacacgg
     4261 attcctttag gtgcaagctg ttttgctaaa ccgatcgtaa agttagcgat tgaagctttt
     4321 gttgctgcgt aatctaataa atgttcgctt ggattgaatg cttgaacaga agttgttgtt
     4381 acgattgctc ctcctgctgg gatatgcgga acagcttctt tcactaaagc aaacatagcg
     4441 atgatgttta tcataaatgt gtctttgact tgctcaagtg gtaactcagc aatagactga
     4501 caagaaattt gctgtcctgc gtttagtacc atcgtatcta gcccgccaag ttcactcacg
     4561 gctttatcta tgatttcttt aggtgcttgt tcgtcacgaa gatcaaaagg tagtaagaca
     4621 atcttacgtc ctgcaacttt cacgtatttt gctacttcct ctgcatcttt ttcttctcct
     4681 gggaagaatt gaagagcgat atctgcccct tctctagcaa atgcaatcgc tgctgcacga
     4741 ccgatccctg aatctccacc agtgattagt acacggcggt tttctagttg attattgcct
     4801 tcataggatt cttctccaca atcaggaaca ggaatcatat tctgctgtaa agctggtgta
     4861 tcctgatctt gtttaggaaa tttctccgta tggtacaagt ttcgcgggtc gattaatttt
     4921 ggttctgtca ttgctgtatt cctcctagtt tttaaattgt attgccaaaa caaatttctt
     4981 tgtttgatac ctgttttaaa tagattcttt aatttgtata atctttccgg tataaatggt
     5041 aacctaaaac tactgctgct atcaaagagc caaagactac gagtataaag cctagagcag
     5101 aacttttacc cggtaatcct cctgtattca ttccccaaat ccctgcgaac aacgccggaa
     5161 cggaaatgat taatgcagct gaatctagat atttcatgat atgatttaga ttattgtcca
     5221 tcatcccgtt gaatagatca ccaatactct ctaataaatc tcgatagatc tcgatcattt
     5281 tttctgtttg tcgctgtctc aaatggacat catagagtag ctgttcatcg tctaaacgtt
     5341 tgatgaaagt ttcagaattc cataattgat ccattgtttc ccgttgatct cttaaggtat
     5401 gatcgatata tacgatgtct ctttcaagat ctgcctgttt atacagttct tcattttccg
     5461 ttgttttacg agctgcttta tccaaagcgt caattcgctt tttcatctct cttagttctt
     5521 tcacgtaatg ggtgtaaata tctaataatg tatacgtgat gatcttttca aacgagttta
     5581 aatgatttcc atgctttgtc agcagccgat ccacaaattc ggtttttcta tcgatacaag
     5641 taatcaagag attatcggaa agtacaaaag atattggcgt taattttttt tcgatacgat
     5701 tatctgactg atctagatta atcactacca aagaaaatgg atgctctagt ttcgtctcat
     5761 aaaggcgctc catccgagaa acttcttccg gatattcggt tcctacgaaa atatcatcag
     5821 gaagctcgaa ttcttcgatt gcttgctcaa tctcttcggg ctttgcacta tcaagtacga
     5881 gccaattata ttttttttca tctgtttcta ttacattctt ttcttcatcc acaaagtagt
     5941 atctcaatgt atacctcctt tttggacttt ctcgaaacta ttagctgaag aagcagttct
     6001 ttaatttgat attataatcg agtactgcga tttcattttc taatgaaaag ctgtcaagct
     6061 gtttgttgaa gtccatgttc aatcgtttac cgaaataatt ttgctctaat ttagatgtat
     6121 agatgtccaa taatggatgc tcgattttag tcgcatattc tccaaattcc gaaacaaaag
     6181 gaatgatttg aaaaaagctg ccttttgcaa aagcaccttt gtcccttaga aatggatcgg
     6241 aatcattgat aagcgcgagt gcgatttttg tattttcccc aaccttttca attaacgtct
     6301 ctactgcatt tttagacatt gtaatttcca ttcctactcc ccctcttcag ttgttgtctt
     6361 ctattattta acaactacat ctgtttttgg ttgaactttt gcatattttg gatatttagg
     6421 gaatttagct acacaacgga aagccgtcat ccctgcatat ttttcgtaat atttcttcgt
     6481 tggcccttca aaatccttgc tccacggttt cacatgtcct gaaaaatgga taatgtaagg
     6541 attgttgcga gtttcttcgt attcacgttc acctgctgct gttggatgtt tttttgcttt
     6601 tgccatgata tagccttgtg cattccaacg cggatgaagt ggtaaccaac ggtcatgtaa
     6661 gatagcattt aatgcatctt ggtcatggaa tcgtaatttt tctggattat gttcgatgaa
     6721 atctaatact ttttgcgtaa tattttcatc taaccatttt ttcacattga tcagcatcaa
     6781 acctgagttg aaataacgca ttgatttagc tgaaattttc attttttcca gtcgttgatg
     6841 gaacccagcg tcttcaacag ctgctacgat tgaatcaccg aagtcaagac gccataattt
     6901 agagatatct tgtaaagcga tcatgtcgca atccatatat aagatacgtt caacttctgt
     6961 gccgcggaac aattccggaa tcgcaatacg ataataagct gtttctggaa tacgatcgct
     7021 gattaccaca tttgtaaaga attctttatt gattttcaag aattccacat ctgagttcat
     7081 tcgtgcgttt ttcactgaga agcgtaaaag ctgtttactt tcaaaatcga tatcatcgtc
     7141 aatcacatag aaccgaacaa atttagacgg gttgcaatta tctaatactg agacaaataa
     7201 tgcagctaaa tgtggtacga attttgtgtt acaacttgcg actactgcta tttctttttt
     7261 attcattgat ctcgcctact ttcgttagag tactgtgtgc taattttttt aagtataaat
     7321 tggtatacgg atgatctttc aacgtattcc aaggtttatc atgtcctgta aagtgaacga
     7381 tcgaaggttt ttcatttcct tctttatact gacgttcata tttttcattt ggtgctggat
     7441 gacgttcaaa aatcagcgaa gtctgcatgt tccattttgg atgaagttgt tcccaatctt
     7501 cataaagtac agcattcaaa gcatcttggt cgtgatagat gattcgatcg ccattttcac
     7561 tgaggtaatg gatagttttt tctgtaattt cttttttatt ccattgatca atgtcaatca
     7621 ccatcactcc tgaattgaag tacaagtcat cggaatcgat tcctaatctt tccaaagctt
     7681 tcacttggcc tggatcgata atagcgccga ttgtttttcc atttaacggc tcatcatata
     7741 gttttacgat atcgtctagt actaacacat ctgaatctaa gtaaaggact ttcttataat
     7801 cctctttagc taataagttt ggtaaagaaa ttcttaagta agcggtagtt gtgatatggt
     7861 cactgactag gaagtcttcg tagatatctt tttccactgt caggaattgg atacttgcat
     7921 tacttgaata tttatttacc gtttcttcca atccttcttt actatattct gatagaccat
     7981 cgtcaatcac ataaaatttt atgcgtcttg ttttattgca attttctaat gcagttgcga
     8041 tcatcacgct caagtaagga gcataatttt cgtctgaagc agttaccaca ggaactacgc
     8101 cgtatctctt ctccatcaaa atcactcctt tttctaatct ttttctaacc tgttctacat
     8161 ctagaatatt cctcatttgg ttttaaagca aaatatatgc tttgttttca ttgaatattt
     8221 taaaattttt ttatcacaaa acgcatcggt aagcgctaac tattcgtttt tcatggcttt
     8281 ttattcaaag gtattgacca aaaactacta tatatagcca cctttttctt aagaatgttt
     8341 tttgaaaaaa ttcataatga caaagcaagt tagtttcatt tttttaaatt ctcatttaat
     8401 cttaaggtaa caggaaaata gatatgtaaa aagaaagtag gaagataaaa tgaaactagc
     8461 agcgattgat ttagacggta ccttgcttga tcgaaatgga aagataccag aagaaaatat
     8521 ccaagcactt cggaaatttg atgaaaatgg cggaatcgta accgtcgcta ccggaaggaa
     8581 cagcatttct gctaaagata ttttttccga attaggaatc ggtggctatt tgatttcttc
     8641 caatggttca ctgattgctg aaatgaagga tggaaaaatg gcgaaagttc tcagacgttc
     8701 aaaaatagat agcgatacac tgagaaaagc cttcttctat gctcaagaag cgaaaatatc
     8761 catcattgct agccgagaaa cccaagatga tcagctgaca ttcgatgaat caaatctagt
     8821 caaagatgac ccttattatg aacatttcaa tttacagaac cattcttttg atcaaatttt
     8881 aaatatgttg gaagatcctt ctcttagcta tcttaaatta gcattaacag ataagagtga
     8941 agaaaaacta cagaagctac aaagagatct gcaagcagat ggtatccaat cagtattctc
     9001 tgatccgcat ttcttagaaa tcacgcctac aggtatcact aaagctcatt cacttgtttt
     9061 tctggcggat taccttggaa tcgaatcgag tgacatcatg gcttttggcg atcaagaaaa
     9121 cgacattgct atgttgaatt acagcgggtt gagtattgcg atggggaatg cacagcgaca
     9181 agtaaaagaa attgcaaacc aagtaacact gacgaatgaa gaagctggtg ttgcttatat
     9241 tttaaaaaac ttctattaaa aagaataaaa tgacgatatg aatgtaggag gaaagaaaat
     9301 gttagttatt attgatatgc aaaatcatat tcttgatcca aacagcgaat tttatattcc
     9361 agagtcagat caattggcag aacgtattgc tcaaagatta gctaaagcac gccaaaataa
     9421 tgaatatatt ctcttcacac gggatatacc agttgaactg aaggacaaag aagaagaacg
     9481 agaagatttg caattgatcc ctcttctttc tccacaagaa aatgaacgag tgattaaaaa
     9541 gtattacttt acgattccac ctgaaacttt agctgaaatc caggaaactt tatttgaaag
     9601 caaagaagaa caaaaagaga tcgaagtagt cgggattgaa acaaacttgt gtgtactctc
     9661 taatctgata ggcctgcaga gtgcttttcc tgaagcagac ttttttgttg atccaatgtt
     9721 agtctccagt agaaaacacg gagaatccgc tttagagctg ttgaaagatt tcaacgtctc
     9781 tatcgtcgaa tcataaaaat atctatcttc atttacgata tttaacacac aataaaaaaa
     9841 gcacgcagaa ctgttttagt cctacgtgct ttttctatca aagtagattg gcttttttat
     9901 gacacagagt gaaacggcag gctcacgacc tctttcttaa atggtgttca atcagctgac
     9961 cctcggtatt agcttaacaa acggcaaaat atgaggaact atttttccgt ttgttaagct
    10021 aatactcagg tcaaaacgct gatttcacaa cctcttcctt aaacggtatt caaaagcttg
    10081 catctgcagt aataagaaca gccaagtaaa aacataagaa gctgttttta cttggctgtt
    10141 cttattactt gatgcagggc agctttttca caacctctta tgtaccttgt aggactcgaa
    10201 cctacgaccg gacggttatg agccgtctgc tctaaccaac tgagct
//
LOCUS       DAZKLM010000093        10018 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3764
            SAMEA115706913-rid22582563.denovo.097, whole genome shotgun
            sequence.
ACCESSION   DAZKLM010000093 DAZKLM010000000
VERSION     DAZKLM010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706913
            Sequence Read Archive: ERR13196647
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10018)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10018)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287823.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287823.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,004
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,849
            CDSs (with protein)               :: 2,849
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 41 of 96
            Pseudo Genes (incomplete)         :: 52 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 15 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10018
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706913-rid22582563.denovo.097"
                     /isolate="VRE3764"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            254..733
                     /locus_tag="ACG842_002279"
     CDS             254..733
                     /locus_tag="ACG842_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6530 family protein"
                     /protein_id="HFQ0248796.1"
                     /translation="MEIPTNLKHKPVIVVEDYDKVDGRNALNMDAKGLSLGLAQWNDR
                     GKVDISGKVWRHTGEKWSRQSEELPITRILDLAILAAQGSLYFQDAYRYEKFYDPENP
                     VVDIIGLQGNRMTVEVDTKNPKIDEDIILYYDTLQKDGELIGERFRILKRLLDELGY"
     gene            766..2994
                     /locus_tag="ACG842_002280"
     CDS             766..2994
                     /locus_tag="ACG842_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFQ0248797.1"
                     /translation="MENFSLTENRTFSRLEKQMIWKKPKSHRTSAEEWRIASEIKGNW
                     QDPEMKISNVLLEGDAGSGKTQLAKALSADLQLPYTKVTCFADMDKSDVFGALLPVVD
                     SNKEQDQELLEAIYQTDTLEAVLTLIQNHYGIDPFSAKEKLAELVQRIEADYPDAIHY
                     SYYPSEIVRAIEKGYLLEIQEPTVIRDASVLVALNSALEPNGMLNLPTGIIRRHPDCV
                     IVITTNRNYQGNRPLNESLRDRMQHAEKMDLPALEVMAERAMAKTMIQKQELLLKMAE
                     IIRLLDETAKANAIKGVAGMRSYFYWANTYKQGQDLLQSIYPKVLYKLSTDSDELAIL
                     EQALSDSGLLLELQDILRKEKWGHPQSEHTKERTISAEEANERNITENAEEILRLDEK
                     KSLSAEEPNEGEEKQEETIEKNEIPPQMQKERSSDIEGKNQKEQQTGDDSQEMSASDM
                     EAHDKEIKKQLNKEARQIMKETIHEKEGLIVHRPAFQGKTPEARELHMQVMPIVESLS
                     RQILELLDNEQTETYQKGKYEGQRFNASKVAYGDLRNFDKKNPPHEQPSLAVAVRIDE
                     SGSMIRDDRIEAAKKAAIAIAEFSKKVDIPLLIYGDTADRSSREKTSLFSYKEFEDGF
                     QWLNEKLVTMKPRQNNRDGAALRLAAEKLNRQSATTKLLLNISDGQPKALPDYTGKKA
                     KEDIQSVLKEYERQGILFVSAAIGQDKEEIKSIYGESRFVDITDLNEFPKQMIQLIAR
                     YL"
     gene            3123..4766
                     /locus_tag="ACG842_002281"
     CDS             3123..4766
                     /locus_tag="ACG842_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HFQ0248798.1"
                     /translation="MIQEQKWWKEAVGYQIYPASFKDSNGDGQGDINGIREKLNYLKD
                     LGIGFIWINPIYQSPFVDNGYDVSDYQDIEERFGTMEDFDLLLKEAHELGIKVIMDLV
                     INHSSDQHQWFEESKKSKESPYRDYYIWVDGVDGKEPNNWTSIFGGSAWEYSHETGQY
                     YLHVFAKEQPDLNWESEKLKEELFNMIRWWLDKGIDGFRLDAISHVKKDEYSVKATEN
                     PFSPFQNVSGIEEHLTDLKHVFEEYDIMTVGEASGVTAEEGPQWVGKDGYFDMIFEFD
                     HIHIWQQEKEGQLDVLKLKHALSAWQTSLDGIGWNALYMENHDVPRAVSVFGDTRPDF
                     WAMSAKAIAMMYFFLQGTPFIYQGQEIGMTNMPFESIDQVDAVDSKRLYKRLLAEGKT
                     REEALDIIRETTRDNSRTPMQWTSEQYAGFSTHEPWLITNPNTKTINVEQQEYEPESV
                     LQFYKNMIRIRQTNKGLIYGSYKEYLHEHPQLYVYERYLEDEQYLIMVNLTESLADYE
                     LPKEADQSWTLLLSNSSSGEFEAKGILAPYEARLYKTNK"
     gene            complement(4839..5384)
                     /locus_tag="ACG842_002282"
     CDS             complement(4839..5384)
                     /locus_tag="ACG842_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HFQ0248799.1"
                     /translation="MKLSEKELKDQLTEIVNDILAEAHLKKGDLFVLGCSTSEVVGGH
                     IGKNSSAEVGQWIIRTLKELLDPKEIALAVQGCEHLNRALVVERTVAEAKNFEIVSVV
                     PALHAGGACSVAAFDQFNDPVEVEHVVGQAGIDIGDTSIGMHIKHVQVPVRPRLKTLG
                     QAHVTALRSRPKYIGGPRANY"
     gene            complement(5407..5892)
                     /locus_tag="ACG842_002283"
     CDS             complement(5407..5892)
                     /locus_tag="ACG842_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFQ0248800.1"
                     /translation="MKTKQLTIYAMLIALTVALSLTILIPVPATNGFVTLCEAGIYTA
                     AFLYGPTGGLVVGASSGLLIDLISGYPQWAVFSLVIHGLQGYVAGSFSKSSTRMWGLG
                     LLLGSLVMIIGYGIAGWILYDWPAGVASIPGNIFQNLIGMGLAFPITASLQRIQARKQ
                     Y"
     gene            complement(5882..6694)
                     /locus_tag="ACG842_002284"
     CDS             complement(5882..6694)
                     /locus_tag="ACG842_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylpyrimidine/phosphomethylpyrimidine
                     kinase"
                     /protein_id="HFQ0248801.1"
                     /translation="MKRILTIGGSDPFAGGGIQTDLKTFENFGLFGLSALTSIGALDI
                     NERFMLESISLELLKRQLVSIDKMTSLDGIKIGLLNSSEAIMVVRDFIKTKQNIPIIL
                     DPVLAFKETEAVINQLYISQLIEELFPLVTMITPNLAEAALLSGQALPSSLTEMIHLA
                     KELTASGTKNIVIKGGTRMAGDEAVDLLYSIDHYEVFSHKKIDTTTVNGAGCAFSSAI
                     TANLVLGADFTKAVAESKKFVAHCISNGVLMNDHTGSVWSGGIQKGGMRNEN"
     gene            complement(6833..8365)
                     /locus_tag="ACG842_002285"
     CDS             complement(6833..8365)
                     /locus_tag="ACG842_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HFQ0248802.1"
                     /translation="MMETITYSPLNLFTNFQEAAANFSETPIIFDEPLSAFPELGLET
                     TYKKCCESVLKRAYQLAHLGVKSGDKVIIYKSSAFDTYLLAVSVSYLGAVPVMTSYHL
                     PTTTMEVFIDRLEDPFILFDDETKKRVREISNGTKSKQIPILYLMEQPALSVSQSFLD
                     KNEISYMTHTSGTTGIPKLICHSAHSMGWRTKWQKTVFTKISEKKLIAFHISPVHSRF
                     NIGISSLMSMGFPMMPLANAQSSKVVHMLEAHRPIALETHPNNFVQWRFTAKEHPEAF
                     SGIRYYHSTFDAINNQTMKTFLRTSMAQDAVFLQVYGQSECGPMILKAHTLESLKTSD
                     ARDMGVGLEDMTSARITDSVGNVLPENTDGHIQLLSKGRALTYYKEDARFQENVYGDW
                     WDSGDYGFMDKHGHLFLKDRQVDLIETINSTLAIEDFLLDSLDFLAEVVIVRDKNHSP
                     QPIIALAPDKEMDWNRWWEQVHELPRLNVPIIRDFDAIPRTATMKVQRLQIEKELKSQ
                     KS"
     gene            complement(8474..9505)
                     /locus_tag="ACG842_002286"
     CDS             complement(8474..9505)
                     /locus_tag="ACG842_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFQ0248803.1"
                     /translation="MDSKTRKQLQEKLKQRTHLLPSVFQQFLEENRSSLSLGSRYEYA
                     KDFHLFTDYLRKVFSEEVDDQLIIHLEKQDYQNFLAKIYRHTRSFQTTANQETEQLFE
                     NSYYGISRKQYSLNHFLRFLYQKGLIEEEISLLGTSLPETTKAAPRYIDAELFKDFEH
                     LFIPIEGFQTSREASFEEKNRPRNLAITAILLYCGLKIHEVVELKRKDVSLTKQILNL
                     NRKENRLNKVPIPKEAMPFLENYFKLTAQNTDENSFVFRSSHDQQISPRTIRQILSKI
                     TVYKNVSPELCRKTYRYYAELYLDDADAVNYLCGNRYLSSHSFQDIWEKMVDFSYHEL
                     AAFVRVSSI"
BASE COUNT         3340 a         1850 c         1927 g         2901 t
ORIGIN      
        1 acaaaaaaga tgttggtgaa gttcaccaac atcaaaaagt atagagccaa aatattccaa
       61 catcatcttg gactttctac atgttactaa tgagcgtgta aaagaacgag atggtgtttt
      121 tatttctatc aatgaagctc tttttctttg caatcctcac ttcaatcctc tataatgtcg
      181 catatagttt agtaaatgag taaactacta atttacttat ttactaaaaa taaaaggatg
      241 aggagttaaa ataatggaaa taccaacaaa tttaaaacat aaaccagtaa tcgttgtaga
      301 agactatgat aaagttgatg gaagaaatgc attaaatatg gatgcaaaag ggctttcact
      361 gggccttgct cagtggaatg atcgtggaaa agtcgatatc tcaggcaagg tatggcgaca
      421 tactggagaa aaatggtcac gacagtcgga agaattacca atcacacgta ttttagattt
      481 agcgatctta gctgcgcaag gaagtttata cttccaagat gcttatcgtt atgaaaaatt
      541 ttatgatcca gaaaatccag tcgtagacat cattggatta caagggaacc gaatgacggt
      601 agaagtagat accaaaaatc caaagatcga tgaagacatc attttgtatt acgatacact
      661 acaaaaagac ggagaactga ttggggaacg attccgtatt ttaaaacgtc ttttagatga
      721 gctaggatat taatagacag agatgatcaa aaggggaaga gaagcatgga gaatttctca
      781 ctaacagaaa accgaacctt ttcacggcta gaaaagcaaa tgatctggaa gaaaccgaaa
      841 agccatcgga caagtgcaga agaatggcgg atcgcctccg aaatcaaagg gaattggcaa
      901 gatcctgaaa tgaaaatcag taacgtactt ttggaaggag atgccgggtc ggggaagaca
      961 caattagcca aagcactatc tgctgatctg caacttccct atacaaaagt gacctgtttt
     1021 gcagatatgg acaaatcaga tgttttcgga gccttattac ctgtagtaga ttctaataag
     1081 gaacaagacc aagaattact agaagctatt taccaaacag atacacttga agctgtactg
     1141 acactgatcc agaaccatta cggcattgat cctttttctg ctaaagaaaa attagcagag
     1201 ctagttcaaa ggatcgaagc tgattatcca gatgcaatcc attacagcta ttacccttct
     1261 gaaatcgttc gagcgattga aaaaggctat ttattagaga ttcaagagcc gactgtcatc
     1321 cgagatgcct ctgttcttgt ggcacttaac tcagcgctcg aaccaaatgg gatgttgaat
     1381 ttaccgacag gaatcattcg tcgccatcca gattgcgtca ttgtgatcac tacaaatcga
     1441 aattaccaag gaaatcgccc attaaatgaa tctttaagag atcggatgca acatgcggaa
     1501 aaaatggatc ttcctgcctt agaagtcatg gcagaacgtg cgatggcgaa aactatgatc
     1561 caaaaacaag aacttctctt aaagatggca gagattatcc gtttgttgga tgaaactgca
     1621 aaagctaacg ccatcaaagg tgtagcagga atgcgttctt atttttattg ggcaaatacc
     1681 tataagcaag gacaagattt actccagtcg atttatccaa aagttttgta taaattatcc
     1741 actgattcag atgaattggc aattcttgaa caagctttgt ctgacagtgg attgctactg
     1801 gaacttcagg acatattgag aaaagaaaaa tggggtcatc ctcaatctga gcatacaaaa
     1861 gaaagaacca ttagcgccga agaagcaaat gaacgaaaca tcactgaaaa tgctgaagaa
     1921 attctgcgct tagatgaaaa gaaatcgttg tctgctgaag aaccaaatga gggagaagag
     1981 aaacaagaag aaacaataga aaaaaacgag attcctcctc aaatgcaaaa agaacgcagt
     2041 agcgatatcg aaggaaaaaa tcaaaaagaa caacaaacag gagatgattc tcaagaaatg
     2101 tctgcttctg acatggaagc acatgataag gaaatcaaga agcaactgaa taaagaagcg
     2161 cgacaaatca tgaaggaaac gattcatgaa aaagaaggtc tcatcgttca ccgaccagct
     2221 ttccaaggaa aaacgccgga agcacgtgag ctacacatgc aagtaatgcc aatagtagaa
     2281 tcacttagcc ggcaaatttt agagttactg gataatgaac aaacagagac gtaccaaaaa
     2341 ggaaaatacg aaggtcagcg gttcaatgca agcaaagtag catatggtga tttgagaaat
     2401 tttgacaaaa agaacccgcc acatgagcaa ccttctttag cggtagctgt acggatcgat
     2461 gaatctgggt caatgatacg ggatgatcgg atcgaagcgg caaaaaaagc agcgattgcc
     2521 attgccgaat tttctaagaa agtcgatatc ccattgttga tttacggaga tacagcagac
     2581 cgttcgtcaa gagaaaagac ttctttgttt tcttataaag aatttgaaga cggatttcaa
     2641 tggttaaatg aaaaattagt cactatgaaa ccaaggcaaa acaatcgtga tggtgctgca
     2701 cttcgtttag cagcagaaaa attaaacaga caatccgcaa caaccaaact gttgcttaat
     2761 attagtgacg gtcagccaaa agcgttgccg gattatacag gcaaaaaagc aaaagaagat
     2821 atccagtctg ttttgaaaga atatgaacgt caaggaatcc tatttgtctc cgcagctatt
     2881 ggccaagaca aagaagaaat caagtcgatc tacggtgaaa gtcgttttgt agatattact
     2941 gacttgaatg aatttcccaa gcaaatgatt caactgattg cccgctatct atgataaaat
     3001 aaagctcaac gaacgcactt agcagattcg ttgagctttt cgtttctagt taccggtaac
     3061 aaggatactt ctaaaataga atcggttata atcaagaggg accgttaaag gagtgaaacg
     3121 atatgataca agaacaaaaa tggtggaaag aagcagtagg ctatcaaatc tatcctgcta
     3181 gtttcaaaga tagcaatggc gatggtcaag gagacatcaa tgggatccgt gaaaaactta
     3241 attatttgaa agatcttggt atcggcttta tctggatcaa tccgatctat caatctccat
     3301 ttgtcgataa tggttatgac gtaagtgact atcaagatat agaagagaga ttcggaacga
     3361 tggaagattt tgatttattg ctaaaagaag cacatgaatt agggatcaaa gtgattatgg
     3421 atcttgtcat caaccatagt tcggatcagc atcaatggtt tgaagaatcg aaaaaatcta
     3481 aagaaagccc gtatcgcgat tattacattt gggtcgacgg ggtagatggc aaagaaccga
     3541 ataattggac atcgattttc ggcggttctg cttgggagta ttctcatgaa actggacagt
     3601 attatctgca tgtttttgca aaagaacaac cagacttaaa ttgggaaagc gagaaattga
     3661 aagaagaact attcaatatg atccgatggt ggttggataa agggatagat ggatttcgtt
     3721 tagatgcgat ttctcatgta aaaaaagatg aatattcagt aaaagccaca gagaaccctt
     3781 tctcaccctt tcaaaatgtc tcaggaatcg aagaacattt gaccgatttg aaacatgttt
     3841 ttgaagagta cgatatcatg actgtcggag aagcaagtgg tgtcactgca gaagaaggac
     3901 cacaatgggt agggaaagac ggttatttcg atatgatatt cgaatttgat catatccata
     3961 tctggcagca agaaaaagaa gggcaattag atgtccttaa attgaaacat gcattaagcg
     4021 cttggcaaac ttcattggat ggtatcggat ggaacgcttt atatatggaa aaccatgatg
     4081 ttcctcgtgc cgtttcagtg tttggagata ctcgtcccga tttttgggct atgtctgcaa
     4141 aagcaatcgc tatgatgtat ttctttttgc aaggtacgcc atttatttat caaggacagg
     4201 aaatcggaat gacgaatatg ccctttgaat cgatcgatca agttgatgca gtagattcga
     4261 aaagacttta taaaaggctg cttgcggaag gtaagacaag agaagaagca ttggatatca
     4321 ttcgtgaaac aacgagagac aacagcagga cacccatgca atggacttcg gaacaatatg
     4381 ctggtttttc tacacatgaa ccttggctga tcactaatcc aaataccaaa acgatcaatg
     4441 tagaacaaca agaatatgaa ccagagtcgg tcctgcaatt ctataagaac atgatccgca
     4501 ttagacaaac caataaaggt ctgatatacg gttcttataa agaatatctt catgaacatc
     4561 cacaacttta tgtctatgaa agatatttag aagatgaaca atatcttatt atggtaaact
     4621 taacagaatc acttgctgac tatgaacttc caaaagaagc agatcagtcc tggacattac
     4681 tcttgtcaaa ttcctctagc ggagaatttg aagctaaagg catactagca ccttatgaag
     4741 ctcggttata taaaacaaac aagtaaaact tcctcgttaa agttcattag ttggactttg
     4801 cgaggaagtt tttcagattg ctctgtcaat tactcatttc aataattcgc tctaggacca
     4861 ccgatatatt taggacgaga gcgaagggct gtcacgtgag cttgtccaag ggttttcaaa
     4921 cgaggtctga caggtacttg gacatgcttg atatgcatac cgattgatgt gtcaccgata
     4981 tcgataccgg cttgtccaac tacgtgttcc acttcgacag gatcattgaa ctggtcaaaa
     5041 gcagcaacag aacaagcacc tccagcatgt agtgcaggaa caacagagac aatttcgaaa
     5101 ttttttgctt ctgccactgt tcgttctact actaatgctc ggttcaaatg ttcacaacct
     5161 tggacagcta gcgcaatttc tttaggatcc aacaactctt ttaacgtacg gatgatccat
     5221 tgaccaactt ccgcactgga attttttcca atatgtcctc caacgacttc gcttgtgctg
     5281 catcctaaaa caaaaagatc accttttttc aagtgtgcct cagccaatat gtcattaact
     5341 atttcagtca attggtcttt taattctttt tctgacaact tcattttcta aaaccttctt
     5401 tttcaattaa tattgcttcc gtgcttgaat acgttgaaga cttgcagtta taggaaaggc
     5461 aagtcccatt ccaatcagat tttggaagat gtttcctggt atagaagcca ctccagccgg
     5521 ccaatcatat aaaatccaac cggcgatacc atagccgatg atcataacta agcttcctaa
     5581 taaaagccct aatccccaca tacgagtaga agatttgctg aaagaaccag caacatatcc
     5641 ttgtaacccg tggatcacta atgaaaaaac tgcccactgc ggatatcctg aaatcaaatc
     5701 gatcaataga ccactggaag caccaacaac taatccaccc gttggaccat aaagaaaagc
     5761 agcggtatag atcccggcct cgcataatgt cacaaatcca ttcgtagccg gtacagggat
     5821 caaaattgtt aatgaaaggg caactgtcaa cgcaatcagc atagcataga tcgttaattg
     5881 cttagttttc atttctcatt cctccttttt gaataccgcc agaccagaca cttccggtat
     5941 gatcgttcat cagcacgcca ttagaaatac aatgggcaac aaatttttta ctttctgcta
     6001 ctgctttggt aaaatctgcc cctaacacaa gattcgctgt tattgcagaa gaaaaggcac
     6061 aaccggcccc gtttactgtt gtagtgtcga tttttttatg agaaaatacc tcataatgat
     6121 caattgaata gagaagatcc acagcttcat ctccagccat ccttgtgccg cctttgatca
     6181 caatgttttt tgttcctgaa gcagtgagtt ctttagcgag atggatcatt tctgttaacg
     6241 aagagggaag agcttgacca gaaagtaaag cagcttctgc caaattaggc gtaatcatcg
     6301 taacaagtgg aaaaagttct tcgatgagct gcgaaatata caattgattg atcacagctt
     6361 cagtttcttt gaatgccaaa acagggtcta aaatgattgg gatattttgt tttgttttta
     6421 tgaagtctcg aacaaccatg attgcttcac ttgaattcaa taacccaatt ttgataccat
     6481 ctaatgaagt cattttatca atcgaaacta actgtcgttt aagcaattcc aaagatatag
     6541 attccagcat aaatcgctca ttaatatcta gtgcccctat agaagtcaag gcgcttagtc
     6601 cgaatagacc aaaattctca aaagttttta aatctgtttg gattcctcca ccagcaaatg
     6661 gatcagaacc gccaattgtc aagattcttt tcataacatt ttcctccatg aataagagta
     6721 taacgaaatt ttcaaaaaag aaaaacagcc aattggcata tttttatctc atccaattta
     6781 aaaaaacaaa aagaaaagaa agaccaaaat ggatctttct ttccttaaat agttaggact
     6841 tttgtgattt taattctttc tcgatttgaa gccgttgcac tttcattgta gctgttcgcg
     6901 ggattgcatc aaaatcacgg atgataggga cgttcaagcg aggaagttca tggacttgct
     6961 cccaccatcg attccaatcc atttctttat caggagctaa agcaataatc ggttgtggag
     7021 aatgattttt atcccttaca atcactactt ctgccaaaaa atctaacgaa tctaataaaa
     7081 agtcttcaat agctaatgta ctattgatcg tttcgatcaa atccacttgc cgatctttca
     7141 aaaataggtg gccatgctta tccataaaac cgtaatctcc gctatcccac caatcgccat
     7201 aaacattttc ctggaaccgt gcatcttcct tgtaataagt caaagcccgt cctttagaca
     7261 gtaattgtat atgaccatct gtgttttcag gtaggacatt cccaactgaa tctgtaatcc
     7321 gagcgcttgt catgtcttcc agtccaacgc ccatatctct tgcatcactt gttttcaaac
     7381 tctctagtgt atgagcctta agaatcattg gtccgcattc actttggcca tatacttgaa
     7441 gaaataccgc atcctgtgcc atcgacgttc gcaaaaatgt cttcattgtc tggttgttta
     7501 tagcatcaaa agtggagtga tagtatcgga tacccgaaaa agcttctgga tgctccttag
     7561 cagtaaaacg ccactgaaca aagttattcg gatgggtctc tagtgcaatt ggccggtgag
     7621 cttccaacat atgaacaact tttgaacttt gtgcgtttgc taaaggcatc ataggaaatc
     7681 ccatggacat caaagaagag ataccaatat tgaatctgga atgcactgga gagatgtgaa
     7741 aagcgattaa tttcttttcc gaaatttttg tgaaaactgt tttttgccat ttcgtccgcc
     7801 atcccataga atgagcagaa tgacaaataa gtttagggat accagttgta ccagaagtat
     7861 gggtcatata agaaatttca tttttgtcta aaaatgattg agaaacagac aaagcaggtt
     7921 gttccattaa ataaagaata ggaatctgtt ttgattttgt tccatttgaa atttctctca
     7981 ctcgcttttt tgtctcatca tcaaataaaa tgaaagggtc ttctaaacga tcgataaata
     8041 cttccattgt tgttgttggt agatgataag aagtcatgac aggcactgca ccaaggtaag
     8101 acacagaaac agctagaagg tatgtgtcaa aagcggaact cttataaata ataactttgt
     8161 ctccggattt cactccaaga tgagccaatt gataagcccg tttcaacaca ctttcgcagc
     8221 attttttgta agttgtttca agtcctaatt ctgggaaagc tgaaagtggc tcatcaaaga
     8281 taatcggggt ctcggaaaag ttagctgcag cttcttgaaa attcgtaaat aagttcaaag
     8341 gggaatacgt tatggtctcc atcattcaca ctccattatc ttttgttaag taatatacaa
     8401 attcaagata atcgtaacat aatcctttat ataataacat agtttattgt gaaaaaaaga
     8461 tcatacattt gttttatatg gaagaaaccc gaacgaatgc cgctaattcg tgataagaaa
     8521 aatcaaccat cttttcccag atatcttgaa aagaatggga tgagaggtat cgattaccac
     8581 ataaataatt taccgcgtca gcatcatcta aatatagttc tgcataataa cgatatgttt
     8641 ttctgcatag ttctggcgat acgtttttat atactgtaat tttgctcaaa atttggcgaa
     8701 tagtccgagg agaaatttgc tgatcatgtg aggaacgaaa aacaaaagaa ttttcgtccg
     8761 tattttgtgc tgttaactta aaatagttct ccagaaatgg catcgcttct tttggtattg
     8821 gtactttatt cagtctgttt tcttttctat tcagattaag aatctgcttg gtcaaagata
     8881 catctttacg tttaagttct actacttcat ggatctttaa tccgcaatac agaagaatgg
     8941 cagtaattgc aaggttcctc ggtctattct tttcttcaaa gctagcttct ctgcttgttt
     9001 gaaagccttc aatcgggata aacaaatgct caaaatcttt gaaaagttct gcatcgatat
     9061 atcgcggagc tgctttggtt gtttccggta aacttgtacc caaaagagaa atctcttctt
     9121 caatcagtcc tttttgatac aaaaatcgta aaaaatgatt taatgaatat tgcttccttg
     9181 aaatcccgta atagctattt tcaaacaatt gctccgtttc ttggttcgct gtagtttgga
     9241 acgaacgagt atgtcgatag attttagcta agaaattctg ataatcttgt ttttctaaat
     9301 ggataatcag ctgatcatcc acttcttcag aaaatacttt tctcaaataa tcagtaaaca
     9361 gatgaaaatc ttttgcgtat tcatagcgac tgcctaatga aagggaagaa cgattttctt
     9421 ctaaaaactg ttgaaagacg gaagggagca agtgtgtacg ttgctttaac ttttcttgca
     9481 gttgttttct agttttgctg tccattacaa attccctcca atagtgttca ttttctatat
     9541 tttaccgcat ttcatcaaat gaagatagac agtttgtctt gatcttagga agcaaatttt
     9601 taactaagta ctagctaaaa gatatgaagt aagaagatac atttttagtt attttattag
     9661 tgcagtcatt cttttgatct tttaattgat ttatcctctt tttagctatg tggttacttc
     9721 atgagcaatg aaatggattt ggttcgttct aatggcagga ggctctttct tttatttccg
     9781 aaaaattaga aaacattttt tcagaatcga ttggctctcc atttattcaa taatggtttg
     9841 tctaacgata ttgttgggat aattcttaaa gaagtattag aaatagttgg tacttcatta
     9901 ctttacatga agatttattc tatggtagga gcaattatat tgagaattaa tcctttttta
     9961 tataagttgt ttgcttattg atggattgga taaattaaac catcacaacg agtggaaa
//
LOCUS       DAZKLM010000094         9953 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3764
            SAMEA115706913-rid22582563.denovo.098, whole genome shotgun
            sequence.
ACCESSION   DAZKLM010000094 DAZKLM010000000
VERSION     DAZKLM010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706913
            Sequence Read Archive: ERR13196647
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9953)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9953)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287823.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287823.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,004
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,849
            CDSs (with protein)               :: 2,849
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 41 of 96
            Pseudo Genes (incomplete)         :: 52 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 15 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9953
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706913-rid22582563.denovo.098"
                     /isolate="VRE3764"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(46..1557)
                     /locus_tag="ACG842_002287"
     CDS             complement(46..1557)
                     /locus_tag="ACG842_002287"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFQ0248804.1"
                     /translation="MKFKELELSPELLKSVERAGFEEATPIQAETIPLALAGKDVIGQ
                     AQTGTGKTAAFGLPMLEKIDPDRHELQGLVIAPTRELAIQTQEELYRLGRDKKVRVQA
                     VYGGADIGRQIRGLKDRPHIVVGTPGRMLDHINRHTLKLGTVQTLVLDEADEMLNMGF
                     LEDIEKIISQVPDQRQTLLFSATMPPAIKNIGVKFMKNPHHVKIKAKEMTADLIDQYY
                     VRAKEYEKFDIMTRLFDVQTPELTIVFGRTKRRVDELARGLEARGYRAEGIHGDLSQQ
                     KRMSVLRSFKSGHLDILVATDVAARGLDISGVTHVYNYDIPQDPESYVHRIGRTGRAG
                     KGGMSVTFVTPNEMDYLHVIENLTKKRMTTLRPPTEKEAFKGQLGAAVEQIETKLAEN
                     GLDKYLQTADKLLEEYSAQDLVALLLKTTAKDPADAVPVKITPERPLPMQKKGYNKNG
                     KRGGGNNRNRRDGGNYRGNKSKGGYSKNNNSQKDGGKRHNDKKRGFVIRSNND"
     gene            complement(1879..3231)
                     /gene="murF"
                     /locus_tag="ACG842_002288"
     CDS             complement(1879..3231)
                     /gene="murF"
                     /locus_tag="ACG842_002288"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HFQ0248805.1"
                     /translation="MNLTIQEIAQAVGSECSALTKITNVEFDSRKITEGSLFVPLAGT
                     RDGHEFIQQAITNGASASFWSLPVEQAPKNFPVIPVADPLKAMQQLATYYLEKVGAEV
                     VAITGSNGKTTTKDLTASVLSEKYKTYKTQGNYNNNIGLPYTILHMPQDTEKIVLEMG
                     MDHANEITELSLMAQPKAAAITMIGEAHIENLGSREGIAKAKMEITDGLVSDGLLVVP
                     ADEPLLTPLTATLVQTVTTFGIGNGDVHAEVTAKGKAQTDFVVEGETFTIPLPGSYNV
                     TNALIAYTIGRFFGLSIPEIRQGLAHVSITQNRTEWLTAGNGAAILSDVYNANPTAMG
                     LVLDTFKNLPTKGRRIAVLADMLELGKESLNMHAQMSEHIDPEKFAMIFLYGEQMHAL
                     KEELNQKYPQLPVFYFEKEKNKLIDAIKQNLEATDSIMLKGSNSMGLFEVVEQLQQMK
                     "
     gene            complement(3384..4460)
                     /locus_tag="ACG842_002289"
     CDS             complement(3384..4460)
                     /locus_tag="ACG842_002289"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HFQ0248806.1"
                     /translation="MKITLLYGGRSAEHEVSILSAFSVLNAIYYNYYQVQLVFITKEG
                     QWVKGPLLTEKPASKDVLHLSWDPSGQTEEGFTGKVINPGEIKEEGAIVFPVLHGPNG
                     EDGTIQGFLETLNMPYVGAGVLTSACAMDKIMTKYILQAAGVPQVPYVPVLKNQWKEN
                     PKKVFDQCEGSLLYPMFVKPANMGSSVGITKAENREELQNALATAYQYDSRAIVEQGI
                     EAREIEVAVLGNEDVRTTLPGEVVKDVAFYDYEAKYINNKIEMQIPAEVPEEVYQKAQ
                     EYAKLAYTMLGGSGLSRCDFFLTNKNELFLNELNSMPGFTEFSMYPLLWENMGLKYGD
                     LIEELIQLGMNRYHQRQSFFEKNE"
     gene            4656..6401
                     /locus_tag="ACG842_002290"
     CDS             4656..6401
                     /locus_tag="ACG842_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/UbiB family protein"
                     /protein_id="HFQ0248807.1"
                     /translation="MAQTNPRSRLAEIIRVFINYNVVPNFVQQKNPEQVKKAFEELGP
                     TFIKIGQMLSVREDLLSSAFTQTFKTLQDSVPSDTFSTVKKTIETELSLSLSDIFDDF
                     SKSPFASASMGQAHRAKLKNGDAVVVKIQHPNIAEEIRLDLQLFERAIPLIKYIPETS
                     VVDLKGVLQEVKRSLINEMDFLKESQNGEQFYQKNNGWKEIRSPKIYDAFCSKKVIVM
                     EEMSGKNLNHLMNAENKTETFITGIQNKQLKQEVAKLLVENFMKQVFDDGFFHADPHP
                     GNLLFHVLTKEEQTQASRKTETVHEKEFGSFAFRASTSAEDPVAPYTINYIDFGMMGH
                     LSAGLRQKLTQAVLALYTKDAYRIEKAVLRLCQQEGSFDESRFHQELTSFLEQYYDSP
                     IDEINLQEVFTHVVTICHQNNLQFDRDITLLLKAFGTLEGVIRVLDPEVSLMEVASPF
                     AQHYFLTHLDVEDTLKQSGLDLLEGMKAAPKIPQQLHHLLEMWTSGQGKVNLELKKQD
                     KLLSRIESMINRLVFGMILAALIVGSSLLVQAAPVENAEVVSLLGIFTYAIAAFVIIF
                     LAIDALIQMYKKRKK"
     gene            complement(6681..7118)
                     /locus_tag="ACG842_002291"
     CDS             complement(6681..7118)
                     /locus_tag="ACG842_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HFQ0248808.1"
                     /translation="MRLFIDGDGSPVKEDVIKLGESYGLDVVIVTSVDHYTTKEYANH
                     VQIVYVDRGADSADYKIVGMVKKNDFLITQDYGLASLVLPKGVRVFHHSGKEFDEANI
                     GTLLEQRYHSGKLRKAGIRTKGPKPFTEKDHMSFRQALKKAID"
     gene            complement(7102..8475)
                     /locus_tag="ACG842_002292"
     CDS             complement(7102..8475)
                     /locus_tag="ACG842_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0248809.1"
                     /translation="MFEKLKRFGRNFTQTTPFILSNTVIFIPYLLFLGLTDSHNWLTI
                     LPFTLFYAFRMTGLFLVNSLRFGLDSYTLLMIALLIGGAGSLLGILGVFYFPLFYFSS
                     VLLGLSAAWLVPANTTVNFHEKTQGFINMDGKKYLFAAIWLFLLYQAIELPAPTQIAA
                     SFTIYTLFYVMAYHTVSHYPRYELDFKDIKRNLVATRELVLFLIFFGLLFLLRNARLL
                     FDERLFDLAIYGFLLLFILFVFVLGHQKKEWKLPSWLNLFTFLNGMLGNFLFLFSSFY
                     VGILYGLEKLSIFMYLPYAAGLIAAKIVGPWILKRLTWSPLAMQLLGLSSSLLVLLIP
                     NGFAIGVFLLSFWHVVTGSWLNKEYYQTDTAIPMDRRIVVKYTTQNKGSVIHQFLLMT
                     LLFVLAKEMGEPIHLLLITLNHESVPIESVQLLILAKWINVGLLLSGLVTVYHLWKKA
                     KKNEAIH"
     gene            complement(8797..9285)
                     /locus_tag="ACG842_002293"
     CDS             complement(8797..9285)
                     /locus_tag="ACG842_002293"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0248810.1"
                     /translation="MEKIKVTENELDELMAVIQEVWPEVFVPIIGQKQVDYMLKTYQS
                     KKQIQKELTEGVSYFLLKSEEKTVGYTAYEEINGKIYLSKLYLLNKVRGQGLTSSVFR
                     WYEELAAQTKEREIFLRVNQGNHQAIEVYKHEGFEITEELISDIGQGFQMVDYKMKKA
                     LA"
     gene            complement(9307..9909)
                     /locus_tag="ACG842_002294"
     CDS             complement(9307..9909)
                     /locus_tag="ACG842_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HFQ0248811.1"
                     /translation="MSKKGSVLQKCLQIMPIIGLFFFFVLILYAYHHGIFRSSTSLQA
                     FIQQFGKYAVMIFVLLQVIQVIIPILPGGISSVAGMLMFGNGFGLLYSCIGLVIGEAI
                     GFLLVRYYGVAFVQLILSPKKYQKFDELLTRKTKDIKKVLVVTLLLPFAPDDLICLVA
                     GLTKLSFREFMQIVIFLKPWSVGVYSMIMLFLFHQAQGHL"
BASE COUNT         2913 a         2120 c         1798 g         3122 t
ORIGIN      
        1 aaaaatgaaa gtttctctta aaaaagagaa actttcattt tatgtttaat cgttatttga
       61 acggattaca aatccgcgtt ttttatcatt gtgacgttta ccgccatctt tttggctgtt
      121 attatttttg ctataaccgc cttttgattt gtttccacga taattaccgc cgtcacgacg
      181 attacggttg tttccgccac cacgtttgcc atttttattg tatccttttt tctgcattgg
      241 caatgggcgt tctggtgtga tcttcactgg cactgcatca gctggatctt ttgctgtcgt
      301 ttttagcaaa agtgcaacta aatcttgtgc agaatattct tctaataatt tatctgctgt
      361 ttgcaggtat ttatctagac cattttctgc aagttttgtt tcgatttgtt caactgcagc
      421 acctaattga cctttgaatg cttctttttc ggttggtgga cgtaaagtcg tcatccgttt
      481 tttcgtcaag ttttcaatca catgcaagta gtccatttca tttggtgtaa cgaaagtcac
      541 agacataccg ccttttccgg cacggcctgt acgtccgata cggtgaacgt agctttctgg
      601 atcttgaggg atatcatagt tgtacacatg agtgacacct gaaatatcta atccacgtgc
      661 agcaacgtct gtcgctacaa ggatatctaa atgaccgctc ttaaatgaac gcaatacgct
      721 cattcgtttt tgttgagaca aatcgccatg gattccttct gcacggtaac cgcgtgcttc
      781 taatccgcgt gccaattcgt ctacacggcg ttttgtacgt ccgaagacga ttgttaattc
      841 aggtgtttga acatcaaaca agcgagtcat gatatcgaat ttttcgtatt cttttgcgcg
      901 gacatagtat tgatcgatca agtcagctgt catttctttc gctttgatct tcacatgatg
      961 tggatttttc atgaatttaa cgccaatatt cttgatagct ggcggcatcg ttgctgagaa
     1021 aagtaatgtt tgtctttgat caggaacttg tgaaatgatt ttttcgatat cttccaagaa
     1081 gcccatattc aacatttcat ctgcttcatc caaaactaat gtttgaacgg ttccaagttt
     1141 caatgtgtgg cggttaatgt ggtcaagcat acgtcctggt gtacccacaa cgatatgtgg
     1201 acgatctttc aagccacgga tctgacggcc aatatctgct ccgccgtaaa cggcttgtac
     1261 acgtactttt ttgtcgcgcc ctaaacgata taattcttct tgtgtttgga tagctaattc
     1321 acgtgttggt gcgataacta aaccttgtaa ttcgtgacga tctggatcga ttttttctaa
     1381 cattggtaat ccaaatgcag ctgttttccc tgtacctgtc tgtgcttgtc caataacgtc
     1441 ttttccagct aatgctagtg gaatcgtttc tgcttggata ggcgttgctt cttcaaaacc
     1501 agcgcgttcg actgatttca acaattctgg tgataattct agttctttaa atttcaaatg
     1561 aatcctccta tatggttgtt aactgaggat gtagcttttt tgctaccccg tcgatttgtt
     1621 cggtcaaagt gtattgtttc tttttgacag tggccacgca cgtttttatc cattcttagc
     1681 ccctgtggca ccaccgaaaa atcactttgc gaagtttagg cattagaaaa gccaaacggg
     1741 tcgcgttcag ctttatcatt attatgacat ctggcttcaa caagttgttt ctatggcaaa
     1801 accagtccaa tttctcttca tctttcggca ataggttatt ctaacatagt ttttcgtttt
     1861 ctgcaagttt acagattttt atttcatttg ctgaagttgt tctaccactt caaataagcc
     1921 catgctgttg ctgcctttta gcataatact atctgttgct tcaagatttt gtttgatagc
     1981 atcaatcaat ttattctttt ctttttcaaa atagaacaca ggtagttggg ggtatttttg
     2041 gttcaattct tcttttagtg catgcatttg ttccccataa aggaaaatca tggcgaattt
     2101 ctcaggatcg atatgttcac tcatttgcgc atgcatattt aaagactctt tccctaattc
     2161 cagcatatct gctaacacag cgattctgcg tccttttgtt ggcagatttt taaaagtatc
     2221 taataccagt cccatggctg ttggatttgc attgtagaca tcgctcaaaa tagctgcacc
     2281 atttcctgcc gttagccatt cggttctgtt ttgagtgata gaaacatggg cgagcccttg
     2341 gcgtatctca ggtatggaca gtccaaagaa gcgcccgatc gtatacgcaa tcaaagcatt
     2401 cgttacatta taacttccag gcagcgggat cgtaaatgtt tctccttcta ctacaaaatc
     2461 ggtttgtgct tttccttttg ctgtcacttc cgcatgaaca tcgccatttc caataccgaa
     2521 tgttgtgact gtttgtacta atgttgccgt taaaggtgtc agcaacggtt catctgcggg
     2581 cacaacgagt aatccgtctg atacaagtcc gtcagtgatt tccattttgg ctttagcgat
     2641 tccttctcgc gacccaagat tttctatgtg tgcttcaccg atcattgtga ttgctgcagc
     2701 ttttggttga gccataagtg aaagttccgt gatttcattg gcgtgatcca tgcccatttc
     2761 caaaacgatc ttttctgtat cttggggcat gtgcaaaatc gtgtaaggaa gaccaatatt
     2821 attgttgtaa tttccttgcg tcttatatgt tttgtatttt tcagacaaga cagacgcggt
     2881 tagatctttc gtggtggttt taccattgct tcctgtaatt gcaacgactt ctgcgcctac
     2941 tttttccaag taataggttg ccaattgctg catggctttc aaaggatctg ctacaggaat
     3001 aactggaaaa ttttttggag cttgttcaac aggcaagctc caaaatgaag cagatgctcc
     3061 gtttgtgatc gcttgctgga taaattcatg tccatctcgc gtaccagcaa gaggaacgaa
     3121 taaacttcct tcggttatct tccggctgtc aaattcgaca ttggtaatct ttgtcagtgc
     3181 agagcattcg ctgcccacgg cttgtgcaat ttcttgtatt gttagattca ttgagatctc
     3241 tcctaaaaaa ggttgtgaaa aaagcgtttg gcctaaacgg cgggttcgca tgactctcac
     3301 ttccaagtaa atttctctat tcgtatttag aagctagaat gaacgcggtt acgcaatcac
     3361 tccagcctct tctttatttc tctttattca tttttttcaa aaaaagattg acgctgatgg
     3421 tatcgattca ttcctaactg gatcagttct tcaatcaaat caccgtattt caagcccata
     3481 ttttcccata agagtgggta catactgaac tccgtaaatc ctggcataga gtttaattca
     3541 ttcaggaata attcattttt atttgtcaaa aagaaatcgc accggctcaa tccgcttcca
     3601 cctaacatcg tgtaagctaa cttcgcgtac tcttgcgctt tttgataaac ttcttctggc
     3661 acttcggctg gaatctgcat ttcgatttta ttattgatat attttgcttc ataatcatag
     3721 aatgctacgt cttttacgac ttcaccaggc aaagtcgtcc gaacgtcttc atttcctaat
     3781 acagcaactt cgatttcgcg cgcttcaatt ccttgttcaa cgattgctcg agaatcatac
     3841 tgataggctg ttgctaaagc attttgcagc tcttctcggt tttctgcttt tgtaatgccg
     3901 acactagaac ccatattcgc cggtttgaca aacatcggat aaagcaaaga accttcacat
     3961 tgatcaaata cttttttagg attttctttc cattgattct taagtactgg tacataagga
     4021 acttgcggca caccagcagc ttgtaaaata tacttggtca tgattttatc catggcacat
     4081 gcactggtca atacgcctgc gccgacataa ggcatattca atgtctctaa gaagccttgg
     4141 atcgttccat cttccccgtt tggcccatgt aaaactggaa aaacgatggc tccttcttct
     4201 ttgatttcgc ccggattgat cacttttcct gtaaagcctt cctctgtctg tccacttggg
     4261 tcccatgaaa gatgcaagac atctttgctg gcaggttttt ctgttagtaa tggacctttg
     4321 acccattgtc cttcttttgt aataaatacg agttgaactt ggtaataatt ataataaatg
     4381 gcatttaaaa ctgaaaatgc ggaaagaatg gacacttcat gctctgcgct gcgtccgcca
     4441 tatagtaaag taatcttcaa aagattgtcc tcctgtattc taaatcgttc agtgatttac
     4501 tctcatattt tagcacaaaa caaaaataaa cgaataactt atttataaaa aaacgagcca
     4561 gcttctaaaa gaacgagcgt catttatttt ttgtcagcta gattatttgt cccgtttttt
     4621 ataaaaagtt atgataaaga aaaggaggga tgatgatggc gcaaacaaat ccacgcagcc
     4681 gcttggcaga aatcattcga gtgttcatca attacaatgt agtgccaaac tttgtacagc
     4741 aaaaaaatcc cgaacaggta aaaaaagcct ttgaagaact aggccccacc ttcatcaaga
     4801 taggacagat gctttctgtt cgagaagatt tgctttcttc tgccttcact caaacattca
     4861 aaacattaca agatagtgta cctagcgaca cattttcaac ggtcaaaaag acgatcgaaa
     4921 cagaactttc tctttcatta tcagatatat tcgatgattt ttcaaaatcg ccttttgcct
     4981 ctgcttctat ggggcaggct catcgtgcca agctgaagaa cggtgacgcg gtagtcgtta
     5041 agatccaaca ccctaacatc gccgaagaga tccgacttga tcttcaacta tttgaacgag
     5101 cgatcccttt gatcaaatac attcctgaaa ctagcgtagt tgatctaaaa ggggtactcc
     5161 aagaagtaaa acgttccttg atcaatgaaa tggacttctt aaaagaaagt caaaatggcg
     5221 aacaattcta tcaaaagaac aatggctgga aagagatccg cagccccaag atttatgacg
     5281 ctttttgttc gaaaaaagtg atcgtgatgg aagaaatgtc tggtaaaaac ttgaatcatt
     5341 taatgaacgc cgaaaacaaa actgaaactt ttatcactgg aattcaaaac aagcagctga
     5401 aacaagaagt tgcaaagctt ttagttgaga actttatgaa acaggtgttt gatgatggtt
     5461 ttttccatgc tgatcctcat ccagggaatc tgctattcca tgtactaaca aaagaagaac
     5521 agacacaagc tagccgaaaa actgaaacag tccatgaaaa agagttcggt tcctttgctt
     5581 ttcgagcctc cacttctgca gaagaccctg ttgcgccata taccatcaac tacatcgatt
     5641 ttggtatgat ggggcatctt tctgcaggac ttaggcaaaa actcacacaa gccgttttag
     5701 cgctgtatac gaaagatgct tatcgaatcg aaaaagcggt ccttcgactt tgtcagcaag
     5761 aaggctcgtt tgatgaaagt cggttccacc aagaactgac aagttttctt gaacagtatt
     5821 atgattctcc tattgatgaa atcaacttgc aagaagtatt cacacatgtc gtgacgattt
     5881 gtcatcaaaa taatctgcag ttcgatcgtg atatcacgct tttgctgaaa gcatttggga
     5941 cattagaagg ggtgatccgc gtcctcgatc ctgaagtttc cctcatggaa gtcgcttcgc
     6001 cttttgctca acattatttt ctcactcatc tggatgtaga agatacattg aaacaaagcg
     6061 gattagactt attggaaggg atgaaggctg cgcctaagat ccctcaacag ctccaccatc
     6121 tattagaaat gtggacttcg ggacaaggaa aagtcaattt ggaattgaaa aagcaagaca
     6181 aattattgag caggatcgaa agtatgatca atcggcttgt tttcggtatg attttagctg
     6241 cattgatcgt agggtcctcc ctgcttgtcc aagcagcacc tgtagaaaac gcagaggtcg
     6301 tttccttatt agggatattt acgtatgcta ttgccgcatt cgtcattatt tttttggcaa
     6361 tcgatgcatt gatccaaatg tataagaaga ggaaaaagta gaaaaaggtt gtgacagaaa
     6421 gaatcagctt caagaaataa gaaggggttt tacataaatt gtttctcaaa tttttgtgaa
     6481 aattcggctt atttttcgaa gaggctactt ctgaaacacc gagaataaaa aatgtgaaaa
     6541 agctgttctg cacccagtaa taagaacaat caaaaaggga acatggaaca catttatcct
     6601 gtttccctgt tccctttttg atgtattcga gcataaataa aataccttcg ttacctacta
     6661 tcttctgtac ttggtaaacc ttagtcaatt gcctttttta acgcttgacg aaaagacata
     6721 tgatctttct ccgtaaaggg ttttggaccc tttgtcctta tgccagcttt tcttaacttt
     6781 ccactatgat aacgttgttc tagaagcgtg ccgatattcg cttcgtcaaa ttcttttcca
     6841 gagtgatgga acactcgcac acccttcggc aatacaagtg aagctaaacc ataatcttgc
     6901 gtgatcaaaa agtcattctt cttaaccata ccgacgattt tataatccgc actatcagct
     6961 ccgcggtcta cgtagacgat ttggacatga ttagcgtatt ctttggtcgt ataatggtcc
     7021 acactggtca cgataaccac gtcaagacca taggattctc ctaattttat gacatcttct
     7081 tttactggag aaccatctcc atcaatgaat agcctcattc tttttcgcct tcttccataa
     7141 atgataaaca gttactaatc cgctcagtaa aagaccaaca ttgatccatt ttgccaagat
     7201 aagcagttgt acactttcta tcggcactga ttcatggttc agcgtgatca ataatagatg
     7261 gattggctct cccatttctt ttgctaaaac aaacaaaagc gtcatcaata aaaactggtg
     7321 gatcacactc cctttatttt gagtagtata cttgacaacg atccggcgat ccattggaat
     7381 agctgtatca gtttgataat attccttatt tagccagctg cctgttacta cgtgccagaa
     7441 actaagcaga aagacaccta tcgcaaaacc atttggaatc aagaggacca ataaagaaga
     7501 actcagtcca agcaattgca tagccaatgg agaccatgtt aaacgtttca aaatccatgg
     7561 tccgactatt tttgctgcaa tcaagcctgc tgcataaggc aaatacataa aaatcgaaag
     7621 tttttctaat ccgtacagta tgccaacata aaaactgcta aataaaaaaa ggaaattccc
     7681 tagcattcca tttagaaaag taaacagatt tagccagctt ggcaacttcc attccttctt
     7741 ctggtgtcct aaaacaaaca cgaacaagat gaacagcagc aagaaaccat aaatagcaag
     7801 atcaaaaagc ctttcatcga ataacaatcg ggcatttctc aaaagaaaca ataagccaaa
     7861 gaatatcaaa aataaaacaa gctctcgcgt agctaccaga tttcttttga tgtctttgaa
     7921 atccagttcg taacgcggat aatgactaac cgtatggtag gccatgacgt aaaaaagtgt
     7981 ataaatagta aagcttgctg caatctgagt cggtgcaggc aactcaatcg cttgatacag
     8041 gagaaatagc cagatagctg caaataagta ctttttccca tccatattga taaaaccttg
     8101 cgtcttttcg tgaaaattga ccgtggtatt agcaggtaca agccatgctg cactcagccc
     8161 tagcaaaaca cttgaaaaat aaaataaagg aaaataaaat acgcctaata ttccaagcaa
     8221 tgaaccagca ccaccaatca gcagagcgat catcagcaac gtgtaactat ctagaccaaa
     8281 tcgtagactg ttgactaaaa atagtccagt catccgaaag gcataaaaca gcgtaaatgg
     8341 caaaatcgtc agccaattat gcgagtctgt tagtcccaaa aataaaagat aaggaataaa
     8401 aataaccgtg ttacttaaga taaagggcgt tgtttgtgta aaattacgcc caaatcgttt
     8461 gagtttttcg aacatgacaa tggctcctta aacagtctta taactaatct gctcttcgat
     8521 tttagcatta aaggggaaat aaaccaattt tacagaatat attcttgtaa aaaaaaatac
     8581 agaaagaagc ataagcttct ttctgcattt ttacggtact tgattactca agcagacaag
     8641 cccttctcca caaaagtctt gcccgacacc cctgcatccg gcaggtgata aagatagaaa
     8701 tcaattatcc tgtgcctcca aaatcaatta ggagctctgt aaaccagagt ccttctcgac
     8761 ccgtcgcata aatttacttt aacacagttc ctatgtttat gcgagagctt ttttcatttt
     8821 ataatcgacc atttgaaatc cttgaccgat atctgaaata agttcttctg taatctcaaa
     8881 tccttcatgc ttataaacct cgattgcttg atgattcccc tgattcactc gcaaaaagat
     8941 ctctctttcc tttgtttgcg cagcaagttc ttcataccag cgaaaaactg aagaagtcaa
     9001 tccttgacca cgtactttat ttagcaaata taatttactt aaataaattt ttccatttat
     9061 ctcttcataa gcagtgtaac cgacagtctt ttcttcgctt tttaataaaa agtaggacac
     9121 accttctgtc agttcttttt gaatctgttt ttttgactga taagttttca gcatgtaatc
     9181 cacttgtttt tgaccaatga tcggcacaaa aacttccggc catacttctt gtatgactgc
     9241 catcagctca tccagttcat tttctgttac ttttattttt tccatacacc cacgtccttt
     9301 tttacattat aaatgacctt gcgcttgatg gaataaaaag agcatgatca tgctatacac
     9361 acccaccgac cacggtttca agaaaatcac aatctgcata aattccctaa aagataattt
     9421 cgtcaaacca gctactagac agatcaagtc gtctggcgca aagggaagca gcaaggtaac
     9481 aactagtact tttttaatat ctttcgtttt tcttgtcagc aattcatcaa atttctgata
     9541 cttttttggc gataaaataa gctggacaaa ggctacgcca tagtaacgga caagcaaaaa
     9601 tccgattgct tccccaatca ctaggccaat acaactatat aaaagcccaa atccattgcc
     9661 aaacatcagc atgcccgcta ctgaggagat ccctcccggc aaaataggaa tgatcacttg
     9721 gatcacttgc aacagaacaa atatcatcac tgcatacttg ccaaattgct ggataaacgc
     9781 ttgcaaagac gtgctcgatc gaaaaatccc atgatgataa gcataaagaa tcaacacaaa
     9841 gaaaaaaaac agaccaataa tgggcatgat ctgaagacat ttttgtaaaa ctgatccttt
     9901 tttagacatc tatattcccc acatcttttt tcatttatta aaactgcctc ctt
//
LOCUS       DAZKLM010000095         9839 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3764
            SAMEA115706913-rid22582563.denovo.099, whole genome shotgun
            sequence.
ACCESSION   DAZKLM010000095 DAZKLM010000000
VERSION     DAZKLM010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706913
            Sequence Read Archive: ERR13196647
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9839)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9839)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287823.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287823.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,004
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,849
            CDSs (with protein)               :: 2,849
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 41 of 96
            Pseudo Genes (incomplete)         :: 52 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 15 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9839
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706913-rid22582563.denovo.099"
                     /isolate="VRE3764"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(34..2180)
                     /locus_tag="ACG842_002295"
                     /pseudo
     CDS             complement(34..2180)
                     /locus_tag="ACG842_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346400.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
     gene            2410..3588
                     /locus_tag="ACG842_002296"
     CDS             2410..3588
                     /locus_tag="ACG842_002296"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HFQ0248812.1"
                     /translation="MKVKVKKRASNRLKKGYPLIQKEDLAHPQKETTQWVELVDSQNQ
                     FIAKGYLGKQNKGIGWVLSQQNIPFDQSFFEEFFVKSKEKRTFFFSDDSTTAFRIFNG
                     EGDGIGGLIVDYYAGYAVFSWYNETLYAHRELLIQAFREIYPEIIGGYEKIRFESVNL
                     PESQFIYGKEAPEPLIVKENGVKFAVYLNEGLMTGIFLDQKEVRGSLAEGFASGKTVL
                     NMFSYTGAFSVAAAMGGAIETTSVDLAKRSLKKTQEQFEVNGLDVDQQKIIVMDVFEY
                     FKYAKRKQFLYDMIVLDPPSFARNKKKVFRVAKDYGELVKDSLDILAEDGILIASTNA
                     ANVSIEQFQTMIEEEFHAKNVSFEQLALYRLPKDFQTTDTFLEGNYLKVFVYQIKNNH
                     "
     gene            3604..4362
                     /gene="aroD"
                     /locus_tag="ACG842_002297"
     CDS             3604..4362
                     /gene="aroD"
                     /locus_tag="ACG842_002297"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HFQ0248813.1"
                     /translation="MVRVKGLNIGSGRPKICAPLIGAVREEVLREAVLAREAGADLVE
                     WRVDHYREVFSFDEVAETLVLIHEMLDGLPLLFTFRTLAEGGEQDISIRQYRDLYEHV
                     IHTGLVDMVDIELFKVESLGKRLIEEIKSLNIPLIISSHDFKETPADPVLLYRLNVME
                     HFGADIGKLAVTPNNERDVLRLMELTRRANAFFSMPIITMSMGNLGKISRLVGGTTGS
                     VMTFGSLSSDSTSAPGQLPVKALKQIIQLLNEPL"
     gene            4385..4870
                     /gene="ybaK"
                     /locus_tag="ACG842_002298"
     CDS             4385..4870
                     /gene="ybaK"
                     /locus_tag="ACG842_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HFQ0248814.1"
                     /translation="MAKKKIVKTNAIRMVEQKKIPYTEHEYEWDESHLSASSVAEQLP
                     ESQSRIFKTLVAVGNVTGPLVAVIPGEAELNLKKLAKVSGNKKVEMLHLKDLEATTGY
                     IRGGCSPIGMKKLFPTYLDQIAESYEQIIVSAGRRGLQMELAPQDICALTSGQFADIK
                     Q"
     gene            complement(4941..6194)
                     /locus_tag="ACG842_002299"
     CDS             complement(4941..6194)
                     /locus_tag="ACG842_002299"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFQ0248815.1"
                     /translation="MEKLYDVIVVGAGTSGMMAAISAAEQGARVLLIEKNKKAGKKLL
                     MTGGGRCNVTNNRPVDDLIAHIPGNGKFLYSTFAQWNNFDIMNFFESQGVHLKEEDHG
                     RMFPVTNKSKTIIEALLNRLKELDVTLLFSTRVEKLIHKEHKIYGIRTEFEEFHAPAI
                     ILTTGGRTYPSTGSTGDGYKIVKRVGHTVTPLYATESPLISDEPYIQEKTLQGLSLQD
                     ITLRVLNKKGRVLTEHTMDLLFTHFGISGPAALRCSSFVNKELEKTGEPVTLSLDCFP
                     TQTKQELIHILTEKSKATKKNLVNAWHGILPERLLVFFLERLEMDHLTGQQASQKQIQ
                     DFVQLCKEFKLSINKTFPIEKSFVTGGGVSLKEIYPKTLESKIIDGLYFAGELLDVNG
                     YTGGFNITAAFATGHVAGMNAGQRA"
     gene            6311..7660
                     /gene="gdhA"
                     /locus_tag="ACG842_002300"
     CDS             6311..7660
                     /gene="gdhA"
                     /locus_tag="ACG842_002300"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HFQ0248816.1"
                     /translation="MTNATEYVQAIQEKLHQKDQGQEEFLQAIDEFMPTVMSFLDTHP
                     EYIEKNILELLTEPERVIQFRVPWQDDEGNWRVNRGYRIQYNSAIGPYKGGLRFHPSV
                     NLSILKFLAFEQIFKNSLTGLPIGGGKGGSDFDPKGKSDNEIMRFCQSFMLELSKHIG
                     PSIDVPAGDIGVGAREIGYLFGEYKRLKKYDTGVLTGKPLDFWGSKIRTEATGYGLVY
                     YVKHLLNEEKDSFNQKTVFVSGSGNVAIYAIEKVQELGGKVVTCSDSSGYIYDPEGID
                     VKLLKEVKEINRQRLTEYANQRPSAIYHAGESVWTLKEKADIALPCATQNEIDESLAE
                     ILVENGIKIVAEGANMPCTVKAVSVLHEAGVWYCPGKAANAGGVAVSALEMSQNVQRL
                     TWEREQVDEQLDQIMETIYHTCRQTAAEYGDEKNLLLGANVAGFEKVAKAMAAEGLV"
     gene            7772..9118
                     /locus_tag="ACG842_002301"
     CDS             7772..9118
                     /locus_tag="ACG842_002301"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HFQ0248817.1"
                     /translation="MAHIHFDYSKVAPFVNEQELGYMQSQVTAAHNELREGTGAGSDF
                     RGWIDLPTNYDKEEFARIKEAAKKIQSDSEVLVVIGIGGSYLGARAAIDFLNHTFFNL
                     LDSNTRKAPQIFFAGNSISSTYIADLIEVIGDRDFSVNVISKSGTTTEPAIAFRVFKE
                     LLVKKYGQEEANKRIYATTDKAKGAVKVEADAEGWETFVIPDDIGGRFSVLTAVGLLP
                     IAVSGADIDALMQGAADASKAYSSDKLEENEAYQYAAMRNILYRKGKVTELLINYEPG
                     MQYFSEWWKQLYGESEGKDQKGIYPSSANFSTDLHSLGQFIQEGRRNIFETVVKVEKP
                     RKSITIPEQAEDLDGLGYLQGKEVDFVNTKAFEGTLLAHTDGDVPNLLVKIPEMDAYT
                     LGYTMYFFEIAVGISGYLNGINPFDQPGVEAYKKNMFALLGKPGFEDLAKELNERL"
     gene            complement(9426..9812)
                     /locus_tag="ACG842_002302"
     CDS             complement(9426..9812)
                     /locus_tag="ACG842_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HFQ0248818.1"
                     /translation="MKKILGLIVIILIAFIGWKGWDYYQSTYVGKDYYAVVKAPMPAE
                     TDIKADNGEVIGRGFKYNVDAYAENGEKRQLDFDVITSGDSANGSAYPEGTILQLKAS
                     EKRIIEKKVITSDKVPSSVKEKLGLS"
BASE COUNT         3141 a         1788 c         2013 g         2897 t
ORIGIN      
        1 aattttgtcg ctgctccttt taaatttatt ttatcattcg gaagaacttg tggtcgttcc
       61 gtgaagttct tggtacgttt gcgttttata aagatcaaca gttgattgtc cgttgtgttc
      121 actaaagaca ctagttgact tgtcaccaag agatttttca gcttctttta atttctctaa
      181 gccattcttg tagtcataat cactggtatc aattgggtct aatccactat tcgtatagaa
      241 ccgaagaaga tcaccattat tgatttggtc agaaacagct aactgtttat caactttttc
      301 tttcagtgaa tcaatttcat tctgtacttc ctcagtaggt tcagtaatag cttcaccagt
      361 ttcgttacta tagatcgtac caccgtagta agtatatttt ggtgtaacaa aatcaccatc
      421 acggaaagaa acaagctgtt catgatcttt cgagaataga tcttgtccta actgaatata
      481 attttttgta tctacaccta gtaagtgaag taatgtaggc aaggcatcaa cttgtccgcc
      541 gtaagtgtga ttgattccgc cctgtgtttg tccagggata tggatcatat aagggacacg
      601 ctgcatttgt gcattgtcgt agtttgtcca agtagaactt gtttttccaa ctaattcagc
      661 tagattttta tttcttgaat tcgagacacc gtaatggtca ccgtaaagga cgatgactga
      721 attatcatac aagccgcttt ctttcaaata attgaagaat tcttgtacgg cggtatctaa
      781 ataattggct gtcgcaaagt aaccgttgat cgtttcatca tttgtattgg ctaacgggaa
      841 tcctgcttcg tcattggtga attttgaata aggataatgg ttagatacag caatgaattt
      901 tgaataaaat ggttgttgca gacgttccaa gtattgtacg gattgttgga aaaatggttt
      961 atcatgcaac ccgtattgga acgaattgtc atcattaaca tcataataag aagaatcaaa
     1021 gaaataatca tatcctaaat tcttgtatgt ctcgtttcga ttccagaatg taccagcgtt
     1081 cccatggaat accgcactaa tgtaaccttg tgtttgactt aaaatatcag gtgcagcttg
     1141 gaaagtattt tttccgccta attgtgtgaa caaggagcct tgattcaagc caaacaatga
     1201 attttccatc agcgtttccg catcgcttgt ttttcctgct tttacttgat ggaaaaagtt
     1261 gtcaaaactg aacgtgctgt tgctgtggta aagactattg atgaaaggtg taacttcatg
     1321 ctggagacca ttctcatctg tcaatttata atcgatcagg aattgctgga agctttctaa
     1381 atggatataa atcacatttt ttcctctagc gagcccgaac aaatcgtcat ttggtgctgc
     1441 ataatgttct tttacataat tttctacttc tttcatatca ttgggacttg cttctgcccg
     1501 cacttggttt gtctggtaag tttgaacacc gtcataaacc atgaaagcat tcaagccaag
     1561 ataaccatga aagcattcaa gccaagatat ttgaccaaat aatcacgaga gaatgttcga
     1621 gtcagcaatt ctggacgatc agcttccgcc ataaaaagat ttcctgagaa aatcataact
     1681 gctaatgtag agatcgcaaa agccattctt gctcggatcg ggcgagggtc caacttgatt
     1741 ttttttgcaa tcagcagaac agtcaagata ataaaatcga tccaatagat cacatcatat
     1801 gggcgaaaca gtcgaatggc actttctccc agaccactcg ctactttccc agcacctaaa
     1861 atcgtgttga tcgtaatgaa gtccgtgaat tctcgataat aagtgacatt cgaaaaaaca
     1921 agcaatgtca aaagaaaata aatgaccatc atcgtgatat aagcaactct agggcgtctg
     1981 atataaagag caattccaag taataacaat gtagttgcaa tagggttgat aaacaagata
     2041 aagtattgaa tgccactttc gattccgaga tggaaatcaa ctaaataggc aaaaatcgtt
     2101 ttcaaccaaa gtaaaacgac tagtaaagaa aaaaatccga gcctcttatt caaaaaggca
     2161 ggtgttttta tacatttcaa gataatcttc ctttctgcac ttcttaatca cagaagtgtc
     2221 ctgaaaccat gttacacagc tcacttattc tcatttattt tactagtatg acaatatagt
     2281 gtcaattgaa accagaagtt tatgctgttt cattaaggtt ttctttaagc ttcatgaaaa
     2341 aatgagcagt aagacaagtc ctatgttttt tgttatactt gggaagagaa agctaaagca
     2401 ggtgaaggaa tgaaagtaaa agtaaaaaaa agagcaagca atcgattgaa aaaaggatat
     2461 ccactgatcc aaaaagaaga ccttgctcat ccccaaaaag aaaccacgca atgggtggaa
     2521 ttagttgatt cgcaaaacca atttatcgca aaaggatatc taggaaagca gaataaggga
     2581 atcggttggg tattgagcca gcagaacatc ccatttgatc aatctttttt tgaagaattt
     2641 tttgtgaagt caaaagaaaa aagaactttc tttttttcag atgattctac taccgctttt
     2701 cgtatattca atggagaagg agacgggatc ggcggtctga ttgttgatta ttatgcaggt
     2761 tatgctgttt tttcttggta taacgaaacg ttatatgcac atcgtgaatt attgattcaa
     2821 gcttttcgtg aaatctaccc ggaaatcata ggaggctatg agaaaattcg atttgaatca
     2881 gtaaatctgc ctgagagcca gttcatttac ggaaaagagg caccggaacc cttgatcgta
     2941 aaagaaaacg gagtaaaatt tgccgtttat ttaaatgaag gattaatgac tgggatcttt
     3001 ttagaccaaa aagaagtacg gggatcatta gcagaaggtt tcgcttctgg gaaaacagtt
     3061 ttgaacatgt ttagttatac cggcgccttt tctgttgctg ccgcaatggg aggggcaatc
     3121 gaaacaacaa gtgtcgatct agcaaaacgc agtttaaaaa aaacacagga acagtttgaa
     3181 gtgaatggtt tggatgtcga tcagcaaaaa atcattgtta tggacgtatt cgagtatttc
     3241 aaatacgcca aaagaaaaca atttttatat gacatgattg tattggatcc accaagtttt
     3301 gccagaaata agaaaaaagt ctttcgcgta gcaaaagatt atggggaact ggtcaaggat
     3361 agtctggata ttttagcaga agacgggata cttattgcgt ctacaaatgc tgcgaatgtc
     3421 tcgatagaac aattccaaac aatgattgaa gaggaattcc atgccaaaaa tgtttctttt
     3481 gaacaacttg ctttatatcg tctgccaaaa gatttccaga cgacggatac ctttttagaa
     3541 ggcaattatc tgaaagtgtt tgtttatcaa attaaaaata accattagaa ggagcgatgc
     3601 agaatggttc gggtaaaggg attgaatata ggaagcggta gaccaaagat ttgtgcgcct
     3661 cttatcgggg ccgtccgaga ggaagtatta cgtgaagcag ttcttgcacg tgaagcaggg
     3721 gccgatttag tcgaatggcg agtcgatcat tatcgtgaag ttttttcttt tgatgaagta
     3781 gcagaaacat tggttcttat ccatgagatg cttgatggac tgccgttatt atttacgttt
     3841 cgtaccttag ctgaaggcgg agaacaagac atatcgatcc gtcagtatcg cgacctttat
     3901 gaacatgtga tacacacagg attagtagat atggtcgata tcgagttgtt caaagtagaa
     3961 agtctaggaa aaagactgat agaggaaatc aagtctttaa acattccttt aattatcagt
     4021 agtcatgatt ttaaagaaac accagcagac cctgtcttgc tttaccgatt gaatgttatg
     4081 gaacattttg gagccgatat cggaaaactg gcagtaactc ccaataatga acgagatgtt
     4141 ttgcggttga tggagttgac tagacgggca aatgcttttt tctccatgcc tattattacg
     4201 atgtccatgg gaaatcttgg taaaatttct cgtctggttg gcggaacgac gggttccgtt
     4261 atgacatttg gttccttaag cagcgattct acttctgctc caggacaatt accggtcaaa
     4321 gcattgaagc aaattattca attattaaat gaaccgttgt agaacaagtt tgaggtgata
     4381 ttttttggca aagaaaaaaa tagtaaaaac gaatgcaatc cgtatggtcg aacaaaaaaa
     4441 gattccctat acagagcacg aatatgagtg ggacgaaagc cacctaagtg cgtcatcggt
     4501 cgctgaacag cttccagaaa gccaatctcg tatatttaaa acactggttg ctgtgggcaa
     4561 tgtgacgggg cctcttgttg ctgtgatacc gggagaagcc gaattgaatc taaaaaaatt
     4621 agcaaaagtt tcagggaata aaaaagtaga aatgctccat ttaaaagatt tggaagcaac
     4681 gaccggatac atacgtggtg gctgctcgcc tataggtatg aaaaaactat ttcctactta
     4741 tttagatcaa attgcagaaa gctatgaaca aatcattgtc tcggctggca gaagagggct
     4801 acagatggag ttagcacctc aagatatctg tgcattaacg agcggacaat ttgcagatat
     4861 aaaacaataa atcatacgta taaaagaaag aaggaggccg ggatgttgtt tgtccagtct
     4921 ccttctttct tttttataca ttatgctctt tgcccggcat tcattcctgc tacatgccct
     4981 gtagcgaaag ccgctgtgat attaaagccg ccagtataac cattgacatc cagtaattct
     5041 cccgcaaaat acagaccatc aatgatttta ctctccaatg ttttaggata aatttccttt
     5101 agagaaaccc ctccgccagt tacaaatgat ttctcaatag gaaaagtttt gttgattgat
     5161 agcttaaact ctttgcatag ttgaacaaaa tcttgaattt gtttttgaga ggcttgttgt
     5221 cctgtcagat gatccatttc aagccgctca agaaagaaaa ctaacaatcg ctcaggcaat
     5281 ataccatgcc aagcattgac caagttcttt ttggttgctt tacttttttc tgtcaatata
     5341 tgaataagct cttgtttggt ttgtgtaggg aaacaatcca gagaaagtgt cactggttct
     5401 cccgttttct ctaattcttt attgacaaaa cttgaacaac gcagtgcagc cggaccagaa
     5461 ataccgaaat gggtgaaaag taaatccatc gtatgttcag tcaatacacg acctttttta
     5521 tttaaaacgc gtaaagtgat gtcttgtaaa gaaagccctt gaagtgtttt ctcctgaata
     5581 tacggttcat ccgatatcaa gggggactct gtcgcataaa gaggcgtcac cgtgtgaccc
     5641 actcgcttca cgatcttgta cccatctcca gtagaaccag tagatggata agtccgtcca
     5701 ccagttgtta agataatggc aggagcatgg aactcttcga attccgtgcg tataccgtaa
     5761 attttatgtt ctttgtggat caatttctct actcgagtag agaatagaag cgtcacatct
     5821 agttctttca gacgattgag caatgcttcg ataatcgttt ttgatttatt tgttacagga
     5881 aacatccgtc catgatcctc ttcttttaaa tgtacgcctt gtgattcaaa gaagttcatg
     5941 atatcgaaat tattccattg cgcaaacgta ctatataaaa actttccatt tccaggaata
     6001 tgtgcaatca aatcatcaac aggacgattg ttcgtaacat tgcaccgacc accacctgtc
     6061 atcagtagtt tttttcctgc ttttttgttt ttttcgatca agagtacgcg tgcgccttgt
     6121 tcagctgcac tgatcgctgc catcatcccg cttgttccag cgccgactac gatcacatca
     6181 tacagttttt ccattccctc acctcaagga aagtgtagca tatctgaaga aagaaaacag
     6241 aaaaaaatgt tttgagccaa aagagaaaat gagctaaaat gagggaagat aagcaaagga
     6301 gcgtggaacg atgacaaacg caacagaata tgttcaagca attcaagaaa agctacatca
     6361 aaaggatcaa gggcaagaag aatttttaca agcaatcgat gaattcatgc ccacggttat
     6421 gtcttttttg gacactcatc ccgaatatat cgaaaagaat atcttagaac ttttaacgga
     6481 accagaaaga gttattcaat ttcgagtgcc ttggcaagat gatgaaggaa actggcgagt
     6541 aaatagaggg taccgtatcc aatacaactc agctatcggc ccttataaag gtggattgcg
     6601 cttccatcct agtgtcaatc taagtatctt gaaatttttg gcttttgaac agattttcaa
     6661 gaatagttta accggtctgc caatcggtgg aggaaaaggc ggaagtgact tcgatcctaa
     6721 aggaaaatct gataatgaga tcatgcgttt ttgtcaaagt ttcatgctgg aattgtcaaa
     6781 acatatcggc ccatcgattg atgttcctgc gggagatatc ggtgtaggag ctagagaaat
     6841 cggctatttg ttcggcgaat ataaacgatt gaaaaaatat gatactggtg tgttgacagg
     6901 taaacctttg gatttttggg gaagtaagat acgtacggaa gctactggtt atggattagt
     6961 gtattatgtc aaacatctat tgaacgaaga aaaagattca tttaatcaaa aaacagtttt
     7021 tgtttccgga agcgggaatg tagccattta tgcaatcgaa aaagtgcaag agctaggcgg
     7081 gaaagttgtt acatgttcag attctagcgg gtacatctat gatccagaag gaatagatgt
     7141 gaaactattg aaagaagtca aagaaatcaa tagacaacgt ttgacggaat acgcaaacca
     7201 acgtccttct gctatttatc atgctggaga atctgtttgg acattgaaag aaaaagcgga
     7261 tatcgctttg ccatgtgcta ctcaaaatga aatagacgaa agcttggccg aaatcttagt
     7321 agaaaatggg atcaaaatcg tagcagaagg cgctaatatg ccatgtacag taaaagctgt
     7381 ttctgttctt catgaagcag gcgtgtggta ttgtccagga aaagctgcta atgctggagg
     7441 agtagcagta tctgctttag aaatgagtca gaacgtccag cgattgactt gggaaagaga
     7501 gcaagtggat gagcagctgg atcaaatcat ggaaacgatt taccatacgt gtcgccaaac
     7561 agcagccgag tacggcgacg aaaaaaatct gcttcttgga gcaaatgttg ctggatttga
     7621 aaaagttgcc aaagcaatgg cagcagaagg tttagtttga aaaatgaaac tttcttggaa
     7681 atattgtcag cattttgttt atttgcttga aaaataataa aaaaaaacgt aaagtattac
     7741 ctgtagagta aacctataag gaggagaaca aatggcacac attcattttg actattcaaa
     7801 agtagcaccg tttgtcaacg aacaagagtt agggtacatg caaagccaag taacagcagc
     7861 ccacaacgaa ttacgggaag gtactggcgc aggaagcgac ttccgcggct ggatcgattt
     7921 accaacgaac tatgacaaag aagaatttgc acgtatcaaa gaagcagcta aaaaaatcca
     7981 atctgattct gaagtattag ttgttatcgg tatcggtggt tcttatttag gcgctcgtgc
     8041 agcgatcgac ttcttgaatc acacattctt caatttatta gatagcaaca caagaaaagc
     8101 accacaaatc ttctttgccg gaaattcaat cagctcaaca tatattgctg acttgatcga
     8161 agtaatcggt gatcgcgact tttcagtaaa cgttatttca aaatctggaa caactactga
     8221 accagcgatt gcgttccgtg tattcaaaga gcttcttgtt aagaaatatg gacaagaaga
     8281 agcaaacaaa cgtatctacg ctacaacaga taaagcaaaa ggtgcagtta aagtagaagc
     8341 agatgcagaa ggttgggaaa ctttcgtgat tcctgatgac atcggcggac gtttctctgt
     8401 attaactgct gttggtttgt taccaatcgc tgttagtggt gcagatatcg atgcattgat
     8461 gcaaggtgcc gctgatgcaa gcaaagcata ctcaagcgac aaactagaag aaaacgaagc
     8521 ttaccaatac gcagcaatgc gcaacattct ttaccgtaaa ggcaaagtaa ctgagttgtt
     8581 gatcaactat gaaccaggaa tgcaatactt ctcagaatgg tggaaacaat tgtatggtga
     8641 atctgaagga aaagaccaaa aaggcatcta tccttcaagc gcaaacttct caactgattt
     8701 gcactcttta ggacaattca tccaagaagg acgtcgcaat atttttgaaa cagttgtgaa
     8761 ggttgaaaaa cctcgtaaat caatcacaat tcctgaacaa gcagaagatt tagacggtct
     8821 aggctacttg caaggaaaag aagtggactt cgtaaataca aaagcatttg aaggaacatt
     8881 gcttgctcat acagacggtg acgtgcctaa cttgctagtc aaaattccag aaatggatgc
     8941 ttatacattg ggttatacaa tgtacttctt tgaaattgct gtaggtattt caggttactt
     9001 gaatggaatc aacccattcg accaaccagg tgtagaagct tacaagaaaa acatgttcgc
     9061 acttctaggc aaaccaggct ttgaagatct tgctaaagaa ttgaacgaac gcctgtaaga
     9121 taagggtttt tcgctagatt taaataatca gtgatagaaa catgttcgct ttaaaatgaa
     9181 aactggtacc catttaggta ctcaaacata ctgcatcgtt tattcagaat aaggggctgt
     9241 gacaagacgt ttgtcacagt cccttgtttg tatagaaatc gattgattgc cttgctcttt
     9301 agtttgtttt aggactttaa gaacagtgag gctgacttcg aaggttaatt ctgttttttt
     9361 gttttctaga atcagtaagc aaaaaagcac tcacttcgag tgcttttttg cgatatattc
     9421 ttagcttaag ataggcctaa tttttctttt acagatgagg gtaccttatc gctggtaatt
     9481 acttttttct ctatgatccg cttttcgctt gctttcaact gcaagatcgt tccttcgggg
     9541 taagcagaac cattggcaga gtcaccagaa gtaatgacat cgaaatctaa ctgtcttttt
     9601 tcaccgtttt cagcataagc atctacgttg tatttaaaac ctcttccaat tacttcccca
     9661 ttatctgctt tgatatcagt ttctgcaggc attggagctt tgactaccgc ataataatct
     9721 ttacctacat aagtagactg atagtagtcc caacctttcc atcctataaa agcaatcaat
     9781 atgataacga tcaaccctaa aatttttttc atgaaagatc tcctttttta tttttatta
//
LOCUS       DAZKLM010000096         9593 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3764
            SAMEA115706913-rid22582563.denovo.100, whole genome shotgun
            sequence.
ACCESSION   DAZKLM010000096 DAZKLM010000000
VERSION     DAZKLM010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706913
            Sequence Read Archive: ERR13196647
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9593)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9593)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287823.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287823.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,004
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,849
            CDSs (with protein)               :: 2,849
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 41 of 96
            Pseudo Genes (incomplete)         :: 52 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 15 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9593
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706913-rid22582563.denovo.100"
                     /isolate="VRE3764"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..641)
                     /locus_tag="ACG842_002303"
     CDS             complement(<1..641)
                     /locus_tag="ACG842_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HFQ0248819.1"
                     /translation="MKNSRWQWMEYAGLFSLFLTLISLAVGVTINFRPLYVFDIGHLQ
                     ILDYTSLDQETLLKNFDHLMNYLNNPFKTILSLPDFPVSASGAHHFYEVKILFLVDYA
                     VFFITLIPSILFIKYLQKNDRLWRLIRPFQIGMLLPVVFGFFMMIGFDRFFILFHETF
                     FNNDDWLFDPVTDPIINVLPEEFFMHSFILFFVLLELFFAVFLFLGKNSLKQT"
     gene            complement(643..1407)
                     /locus_tag="ACG842_002304"
     CDS             complement(643..1407)
                     /locus_tag="ACG842_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HFQ0248820.1"
                     /translation="MDYKGYLIDLDGTIYRGTQPIPAGKRFVEELQKRKLPFLFVTNN
                     TTKSPETVANRLADEFDIHVAPETVYTATLATIDFMKADGKGKKVYVIGEAGLIDLIL
                     AAGFTWEEEAPDYVVVGLDNYLTYEKVVKATLAIQKGATFIGTNPDKNIPTERGLLPG
                     AGSVISFVETATQTPPIYIGKPEAIIMDKAVEVLGLQKEEVIMVGDNYETDIQAGIRN
                     NIDTLLVLSGFTKEEDVPGLPIPATYVKQSLDEWSF"
     gene            complement(1415..2092)
                     /locus_tag="ACG842_002305"
     CDS             complement(1415..2092)
                     /locus_tag="ACG842_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YutD family protein"
                     /protein_id="HFQ0248821.1"
                     /translation="MSAKQNEQQNVEHKTEIAKKKSSAEQETTVTEELTAALEEIVEE
                     PKAEKVKGELVTLLDDSNFLIGERHYRLVSDYREGFNAEKLGERYSDVLARYDYIVGD
                     WGYEQLRLKGFFQADNRRAHPDQRIDSLEDYLYEYCNFGCAYFVIERIGGKKEKTTQR
                     RKKKKNHNRAYIDEKKGPVASNRKKPVIKNRKTESAKTSEKVKKQEQPKQVEKTKTAF
                     TIRKREE"
     gene            complement(2129..3514)
                     /locus_tag="ACG842_002306"
     CDS             complement(2129..3514)
                     /locus_tag="ACG842_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HFQ0248822.1"
                     /translation="MEKIVILHTNDLHSHLENWPRIRRFLDQRKRENEKKEKTTTIAV
                     DLGDFVDRWHPLSEATNGQENILLMNEVGYDAATIGNNEGVGNSKEELNHLYDHAEFD
                     IILDNLFDKNTLQPPAWTKPYKIITTKHQTKIGLIAFTAPFPLTYNPNGWDIRNPYDI
                     LPELVEDLRSQVDVLVLMSHLGIQDDFQIAKEIPAIDVILGSHTHHLFREGHVINDVQ
                     IAAAGKYGQYVGEVHLTIDEEKKILKHSAKAIPTETMTAFTEDEQEINSYLERGHELL
                     KEKEVADLPFSLSLDIFNEHSFIQVAFEAVKERGKTEAAILNSGLFLTEIPKGRVNQD
                     QLHTALPHPMHLLNVTLGGNDLIRLVLEIEKNRNFLRNYPMKGMGFRGKIFGQMVYNG
                     ITYDSINHQVFWKNKPIDLNKNYTFTTVDHLMFVPFFPTIEIAGKNEFLFPEFIRSVV
                     GDYLKAHYPIK"
     gene            complement(3724..3808)
                     /gene="ffs"
                     /locus_tag="ACG842_002307"
     ncRNA           complement(3724..3808)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="ACG842_002307"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(3831..4346)
                     /gene="tadA"
                     /locus_tag="ACG842_002308"
     CDS             complement(3831..4346)
                     /gene="tadA"
                     /locus_tag="ACG842_002308"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HFQ0248823.1"
                     /translation="MVNTNLDAVEKEKWMRLALAEAKKAEMLHEVPIGAVVVLDGKVI
                     GRGYNLRETTQDATTHAEMLAIKEACEKVGSWRLEDAALFVTLEPCPMCSGAMILSRV
                     AEVYFGAYDPKGGTAGTLMNLLEDERFNHQAYVEGGILEDECGMILTDFFRKLRQRKK
                     KLKKDWSTNEK"
     gene            4453..5310
                     /locus_tag="ACG842_002309"
     CDS             4453..5310
                     /locus_tag="ACG842_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0248824.1"
                     /translation="MKMDKLLALYPQAQKKNSPASEADILSFAIDDAFIWIKQDSLSQ
                     QETSLLKALFPVINDQKKHPWYRYLFKDASCVQEKSFRLIQLHVESKGEFLKKEWQDT
                     ILELFRYSADFFFYTDTDALLVEQINRDYLDATEINGIFLSLDADFDTTTSVFVGSFH
                     SPGTDLVPLFAEERKIFLTEQNNLYTHSRTFSMQDVALHYYTKDTVKDSKLIKSYIEL
                     IKQNEMEVIIQELWNELGNVSSAAKTLFLHRNTLKYRIEKFQEQSGFNLKKANDLLFC
                     YLLLLHEHN"
     gene            complement(5391..5726)
                     /locus_tag="ACG842_002310"
     CDS             complement(5391..5726)
                     /locus_tag="ACG842_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0248825.1"
                     /translation="MKTSTKVTIGLSVAAAASVATAVVVSGKVMEKIHHMTTRTKVKK
                     FVYDKFDGNEKLLEIVDGLSDSDLDSLMGMLAKIKSGKKKISVYGDSIKDSTEDVKDR
                     LMHFVEKMM"
     gene            complement(5793..7163)
                     /locus_tag="ACG842_002311"
     CDS             complement(5793..7163)
                     /locus_tag="ACG842_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmF rRNA methyltransferase first C-terminal
                     domain-containing protein"
                     /protein_id="HFQ0248826.1"
                     /translation="MKEEATTLPQQFIKKYRLLLGEEASDFFSALEQGSVKKGFRWNP
                     LKPAGLDMVQTYHSEELQPAPYSNEGFLGTVNGKSFLHQAGYEYSQEPSAMIVGTAAA
                     AKPGEKVLDLCAAPGGKSTQLAAQMKGKGLLVTNEIFPKRAKILSENIERWGVSNAIV
                     TNHAPAELVPHFSGFFDRIVVDAPCSGEGMFRKDPNAIKEWTEESPLYCQKRQQEILS
                     SAIKMLKNKGQLIYSTCTFAPEENEEIISWLVENYPVTIEEIPLTQSVSSGRSEWGSV
                     AGLEKTIRIWPHKDQGEGHFVAKLTFHGQNQMHKEKKTRKKSKVQMTKEQEKLWTEFS
                     NDFHYEATGRLLVFNDHLWEVPELAPSLDGLKVVRTGLHLGDFKKNRFEPSYALALAT
                     KKIENIPCLPITQKEWQSYTAGETFQRDGNQGWVLLVLDKIPVGFGKQVKGTVKNFFP
                     KGLRFH"
     gene            complement(7241..7777)
                     /locus_tag="ACG842_002312"
     CDS             complement(7241..7777)
                     /locus_tag="ACG842_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFQ0248827.1"
                     /translation="MMGRVTNYSKEYLLFKSMVYVEEYGMKSITARELAEFCGCSTYP
                     IYTHFKSISGLKEKILEEITVYFENYLAECSSDDVLSIVYLLKDFFSIHESIREIIAK
                     SDLDMDSLFKRPFFSYVKGHTKIKEEKSLELVWLNALGSLRSDDSSQDFLEMMNAVWD
                     KNEVTDEYLENILKEQSP"
     gene            8086..9567
                     /locus_tag="ACG842_002313"
     CDS             8086..9567
                     /locus_tag="ACG842_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0248828.1"
                     /translation="MLIEARKKELLVLQMVIQKNNCSLQELSNDLHIPKRTIKELIRK
                     INVSLKQFLLIDEFIYSNHKGEIQTKETDQTKKLAVFSKLKLCYLKESNRFNYLMLLI
                     NYPQTAVPKKYLLEQLYISPSYLEKLTKQLNHSLKKFHLRILSVKNCCTLEGNELSIR
                     LYLYFFLSDTFGGNDWPFSSLELNALKMNSTDEQIEKISKQEKNIYLLLTLFILRNKQ
                     QAYFPKTSSEITELMDLLQHTHDYSEYFTSFSVSRPCEETETERAYFNYFFYCFFPNQ
                     ISFRQKQEIGFLFSTSKNLICRQVGQLMTYLTQQFPLNHLENKIPIYFYFLVLAVVFF
                     ELLGESSSSFSTLCFPEATSNHSSSFIQEKKLREKIDTIFSKNGERNQISQYLSQLIQ
                     HLLQTETKEQLLIHLQITKQFTGNYHLQSHLQQLFRKDAVKITAYSEHADLIITDQHE
                     KISDETETFYLDTTHNTVLFGKLIKAVHELYMKKGYENKTKRM"
BASE COUNT         2908 a         1969 c         1502 g         3214 t
ORIGIN      
        1 tttgtttgtt tcaagctatt ttttcctaag aaaagaaaaa cagcaaaaaa taattctaat
       61 aatacaaaaa ataagataaa gctatgcatg aaaaattctt ctggaagtac attgatgatt
      121 ggatcagtaa cgggatcgaa taaccagtca tcgttgttga aaaacgtttc atgaaaaagg
      181 ataaaaaagc gatcaaatcc tatcatcatg aaaaaaccga aaacaactgg cagtaacatg
      241 ccgatttgaa acggacgaat caaccgccaa agccgatcat tcttttgcaa atatttgata
      301 aacaaaatac tcgggatcaa agtgatgaaa aacacagcat aatccactaa aaacaaaatt
      361 ttcacttcat aaaagtgatg ggctccgcta gcagaaactg ggaaatctgg caagctgagg
      421 atggttttga agggattatt gagataattc attaggtgat caaaattttt cagtaacgtc
      481 tcttgatcta aagaagtata atccaaaatt tgcaaatgtc cgatatcaaa tacgtatagt
      541 ggtcgaaagt tgatcgtcac accaactgcc agactgatca gtgtcagaaa taggctgaac
      601 aatccagcat attccatcca ctgccatctc gaatttttca tattagaagc tccattcatc
      661 cagtgattgt ttcacatagg tagctgggat aggcaaccca ggtacatctt cttcttttgt
      721 aaaacctgat aaaacgagca aagtatctat attattgcgt atcccagctt ggatatctgt
      781 ttcatagtta tctccaacca tgattacttc ttctttttgc agccctaata cttcaacggc
      841 tttatccata atgatggctt ctggttttcc gatgtagatc ggcggcgttt gggttgctgt
      901 ctctacaaaa gagatgactg accctgctcc aggaagaagg ccgcgttcag taggtatatt
      961 cttatctggg ttcgttccaa tgaaagtagc acctttttga atagcaagtg ttgctttaac
     1021 tactttttca taggtcagat aattatctaa gcctaccact acatagtcag gagcctcttc
     1081 ttcccatgta aatccggcag ctaaaatcaa gtcaatcaat cctgcttctc cgataacata
     1141 gacttttttc ccttttccat ctgctttcat aaaatcgatt gttgccaaag tagctgtgta
     1201 gactgtctct ggagctacat gaatgtcaaa ctcatctgct agacgattag ctactgtttc
     1261 tggtgactta gtcgtgttat tggtaacaaa caagaaaggt aattttcttt tttgcaattc
     1321 ttccacaaat cgtttaccgg ccggtatggg ttgtgtccca cgataaatcg ttccgtctaa
     1381 atcaattaaa tatcctttat agtccattca tccactactc ttcccttttt ctgatcgtaa
     1441 atgctgtttt tgttttttct acttgtttag gttgttcttg tttttttact ttttcagagg
     1501 tttttgctga ctctgttttt cgatttttaa taaccggttt tttgcgatta gatgcgactg
     1561 gtcctttttt ctcatcgata tacgcacgat tatggttttt tttctttttt cggcgctgag
     1621 ttgtcttttc ttttttaccg ccgattctct cgatgacaaa atacgcacaa ccaaaattgc
     1681 aatattcgta aagatagtct tccaaggaat cgattcgctg atctggatgc gcccgtcgat
     1741 tatccgcttg gaaaaatcct tttaggcgta gttgttcata accccaatct cctacgatat
     1801 aatcatatcg ggctagtaca tcactgtaac gttctccaag cttttctgca ttaaatcctt
     1861 cacgataatc ggacacaagc cgataatgtc tttcacctat caaaaaatta ctgtcatcta
     1921 acagtgtcac tagctcgcct ttgacttttt ctgctttagg ctcctcaacg atttcttcca
     1981 aagcagctgt cagttcttcg gtgactgttg tttcctgttc agctgatgat tttttcttag
     2041 caatttctgt tttatgctca acgttctgct gttcattttg ttttgcagac atgcgttcac
     2101 cacctatctt ttttattata ccttgttttt atttgattgg gtagtgagct ttcaagtaat
     2161 ctcctaccac acttcggata aattcaggga acaaaaattc attttttcca gcaatctcaa
     2221 tcgttggaaa aaatggaaca aacatcaaat gatctacggt agtaaaagta taatttttat
     2281 tcagatcgat tggcttgttc ttccaaaaga cttgatggtt aatagaatca tatgtgattc
     2341 cattatagac catctgccca aaaatcttac cgcgaaatcc cattcccttc ataggatagt
     2401 tacgtaaaaa gttacgattt ttttctattt ctaaaactaa gcggatcaga tcatttccac
     2461 ccaatgtgac atttaacaag tgcatcggat gcggcaacgc agtgtgaagc tgatcctgat
     2521 tgacccgccc ttttggaatt tcagttaaaa acagaccact gtttaatatt gctgcttccg
     2581 tctttcctcg ttctttcacc gcctcaaagg ctacttggat aaacgaatgc tcattgaaga
     2641 tatctagact cagagaaaat ggcaaatctg ctacttcttt ttcttttaat aattcatgcc
     2701 cacgttctag ataactattt atttcttgtt catcttctgt aaaagctgtc attgtttcgg
     2761 tagggatggc tttggctgaa tgcttaagga tttttttctc ttcatcgatg gttaaatgga
     2821 cttcaccgac atactgcccg tactttccag cggctgctat ttggacatca ttgattacgt
     2881 gaccttctct gaataaatga tgggtgtgcg atcctaaaat aacatcaatt gctggtattt
     2941 cttttgcaat ttggaaatca tcctgaatcc cgagatgact catcaaaact aaaacatcca
     3001 cttgtgaacg taaatcttcg actaattctg gcaaaatatc ataaggattc cgtatgtccc
     3061 agccattcgg attataagta agcggaaatg gagcagtaaa tgcaattagt ccgattttcg
     3121 tctggtgttt cgttgtaatg attttatatg gtttcgtcca tgccggtggc tggagcgtat
     3181 ttttatcgaa taagttatcc aggatgatat caaactcagc atgatcgtat aaatgattca
     3241 actcttcttt ggaatttcct acgccttcat tgtttcctat cgtcgctgca tcataaccaa
     3301 cttcattcat cagcaagata ttttcctgtc cattcgttgc ttctgacaat ggatgccaac
     3361 gatccacaaa atcacctaga tctactgcaa tcgttgtcgt tttttctttc ttttcgtttt
     3421 ctcgtttacg ttgatccaaa aaccttctga tccttggcca attttctaaa tgagaatgca
     3481 gatcattcgt atgtaaaatc actatttttt ccatgtaccc tctcgcctct ctttatcttt
     3541 atcataccat aggcagcttt ttcttccatt gaaacaagct ctatttcaga gtttttcctg
     3601 ccttgaagca aattgtataa gtacaatatt cgataaatca gtctggctaa aaaatcagga
     3661 acattcattt ttacagtact ataaaaataa aaaaaacaga cccgaaagtc tgttgtcgtt
     3721 tcattataat cagacacatg gcggaacctg cttagtgctg cttccttcca gacctgacac
     3781 gcttcacaag cccaccattg tcctagcctt tcggcaaaga atagtatacc ttatttttca
     3841 ttcgttgacc agtctttttt taatttcttt tttcgctggc ggagctttcg gaaaaagtca
     3901 gtcaaaatca tcccacattc gtcttctaaa atgccacctt ctacatatgc ttggtggtta
     3961 aaccgctcgt cctccaaaag attcatcaag gtaccggctg ttccgccttt tggatcatac
     4021 gcgccgaaat aaacttctgc tacacgagac aaaatcatcg cgccgctgca cataggacaa
     4081 ggctctaagg taacaaaaag tgcagcatct tccaagcgcc agcttcccac tttttcacag
     4141 gcttccttga ttgcaagcat ttctgcatgt gtagtagcat cttgcgtggt ttctctaagg
     4201 ttatatcctc ttccaatcac ttttccatcc aatacaacca ctgctccaat cggaacttca
     4261 tgaagcattt ctgctttctt tgcttctgcc aatgcaaggc gcatccattt ttctttttct
     4321 actgcatcaa gatttgtatt taccaattac ccttccctgc ctttgttttt tcttgtttta
     4381 ctttagcata tgataaaaag gctgtaacta actttttttt ctatggtaaa ataaatacaa
     4441 gaggtgaaca agatgaaaat ggataaatta cttgctcttt acccacaagc acagaaaaaa
     4501 aactctccag catcagaagc ggatatcctt tcttttgcaa ttgatgatgc ttttatctgg
     4561 atcaaacaag attcattatc tcaacaagaa accagccttt tgaaagcgtt attccctgta
     4621 atcaacgatc aaaaaaaaca cccatggtat cgctatcttt ttaaagatgc ttcttgtgtt
     4681 caagaaaaaa gctttcgttt gatccagctt cacgtcgaat caaaaggaga attcttaaaa
     4741 aaagaatggc aagatactat tttggaatta tttcgctatt ctgctgattt ctttttttat
     4801 acagacacag atgcgctctt agtcgaacaa ataaaccgtg attatcttga tgctactgaa
     4861 atcaacggta tttttctatc tttagatgct gattttgata cgacaacgtc tgtattcgtt
     4921 ggttctttcc attcacctgg caccgacctc gtccctttgt ttgctgaaga aagaaaaatt
     4981 tttttaacag agcaaaacaa tctttatact cacagccgta ctttttctat gcaagatgtg
     5041 gctttacatt actatacaaa agatactgta aaagatagta agttgatcaa aagttacata
     5101 gaactgatca aacaaaatga aatggaagtg atcattcaag aattatggaa tgaattaggc
     5161 aatgtcagct ctgctgcaaa aacactgttt cttcatcgta atactctaaa atatcgaatc
     5221 gaaaaatttc aagaacagtc tggtttcaat ctaaaaaaag ccaacgattt actgttttgc
     5281 tatcttttat tgcttcatga acataattaa agatagatta aaggggttgt gacaataatt
     5341 ttgtcacaac cccttatttc gaatgaatag tgttcaaaag ctagtttctc ttacatcatt
     5401 ttttcaacaa aatgcattag ccggtctttt acatcttcgg ttgaatcttt gattgaatca
     5461 ccataaacag agattttctt ttttcctgat ttgatttttg ctaacattcc catcaaagaa
     5521 tccaagtcac tatctgaaag accatctacg atttctagta atttttcatt tccatcaaat
     5581 ttgtcataga cgaatttttt tactttcgtc cgagtagtca tatgatggat cttttccatt
     5641 acttttcccg atacaacgac tgctgtcgct acactggcag cggctgctac gcttaatccg
     5701 attgttactt ttgttgatgt tttcataagt atcagctcct ttttatctat ctccatcata
     5761 gcatagacaa aaaaataaac gaactaatcc actcagtgaa atctcaatcc tttagggaaa
     5821 aagttcttta ctgttccttt tacttgtttc ccaaatccga caggtatctt atctaatact
     5881 agcaaaaccc atccttgatt cccatcacgt tgaaatgttt ctcctgctgt gtaagattgc
     5941 cattcctttt gagtgatcgg caagcaaggt atattctcta tcttttttgt agcaagcgct
     6001 aatgcatagc taggttcaaa tcgattcttt ttgaaatctc ctaaatgcaa acctgtacgt
     6061 acgactttta aaccatcgag agaaggggct aactcaggaa cttcccaaag atggtcgttg
     6121 aacactaaaa gtcttccagt agcttcataa tgaaaatcat ttgaaaactc cgtccagagt
     6181 ttttcctgct cttttgtcat ttgcacttta cttttttttc gtgtcttctt ttctttatgc
     6241 atctgatttt gtccatgaaa agtcagcttt gcaacaaaat gtccttcacc ctgatcttta
     6301 tgaggccata tacgtattgt tttttccaaa ccagctacgc ttccccattc agaacgtcct
     6361 gaactaacgg attgagtcaa aggaatctcc tcaatcgtta ctggataatt ttctacaagc
     6421 cagctaatga tctcttcatt ttcttctggt gcaaacgtac aagtcgaata aatcagttgt
     6481 cctttatttt ttagcatttt tattgctgaa gacaaaatct cttgctgacg tttttgacag
     6541 tatagcggag attcttctgt ccactctttt attgcatttg gatcttttcg gaacattcct
     6601 tctcctgaac aaggagcatc aaccacgatc cgatcaaaaa agcccgaaaa atgagggaca
     6661 agttctgcgg gtgcatgatt tgtcactatt gcgttcgaaa ctccccatcg ttctatattt
     6721 tctgataaaa tcttcgcacg tttcggaaag atctcattcg tcactaataa ccctttcccc
     6781 ttcatttgag cagctaattg ggttgattta ccaccgggag ccgcacacaa atccaaaact
     6841 ttttcaccgg gttttgctgc agcagctgtt ccaacgatca ttgcacttgg ttcttggcta
     6901 tattcatacc cagcttgatg gaggaaagac tttccattga cagtgccaag aaatccctca
     6961 ttgctgtagg gagcgggttg taattcttcg ctatggtaag tttggaccat gtccaatcct
     7021 gccggtttta gtggattcca ccgaaatcct tttttcacgc ttccttgttc aagcgcagaa
     7081 aaaaaatcgg atgcttcttc gcctaaaaga agacgatatt ttttgataaa ttgctgtggt
     7141 aacgttgtcg cttcctcctt cattccaatg tttcgttaga cattttacaa tcttttttac
     7201 gaaaaataaa ggtggctgat ctactttttc atgttgctgc ctaaggtgac tgttccttta
     7261 agatattttc caagtattca tccgtcacct cgtttttatc ccaaacagcg ttcatcattt
     7321 caagaaaatc ttgagaagaa tcatctgagc gcaaacttcc caatgcatta agccatacca
     7381 attcaagcga cttttcttct ttgatcttcg tgtgaccttt gacatatgag aaaaatggcc
     7441 gcttaaatag cgaatccatg tctaaatcag attttgcaat gatttcacga atactttcat
     7501 ggatagaaaa aaaatctttc aataagtaga caatgcttaa aacatcatcg gaactgcatt
     7561 cagctaagta gttttcaaaa taaacagtga tttcttctaa tatcttctct tttaacccgc
     7621 tgattgattt gaaatgtgta taaatcgggt atgtagaaca accgcagaac tctgctaact
     7681 ctcttgcagt aattgacttc attccatatt cttccacgta taccatactt ttgaacagca
     7741 aatattcttt actataattt gttactctcc ccatcattct tacctccgtc tcttttcatc
     7801 tacccataaa taaataacat aaataattct aaaaatatta ttgataatta tcaaaacata
     7861 tttagttata tccattaaaa atcaattttt ccgccttaaa aacaagaata tcttgacaaa
     7921 ataccctagg aaataaacaa tagaataaac aaggtaacaa aaaaattaaa taacgttata
     7981 taaaaaagaa ggcgacactc tgtgagaata acacagactg aaatgacttt ccactcacag
     8041 tttttgtcta actatataca aaaacagaat aaaaaggagg tatttatgct aattgaagca
     8101 agaaaaaaag agttacttgt tctacaaatg gtcattcaaa aaaataattg ttctttacaa
     8161 gaattatcca atgacttaca tattccaaaa cggacaatca aagaactgat ccgaaaaata
     8221 aatgtatcac tcaagcaatt tttattgatt gatgaattta tttattctaa tcataaagga
     8281 gagatccaaa ccaaagaaac cgatcaaact aaaaagttag ctgttttttc taagttaaaa
     8341 ttatgctacc taaaagaatc caatcgtttt aactatttga tgttactaat caattatcca
     8401 caaactgctg taccaaaaaa atatttactt gaacaacttt atatatcgcc ctcctatctc
     8461 gaaaaactga caaaacagct caatcattcc ttaaaaaaat tccatttacg gatcctatct
     8521 gtaaaaaatt gctgtactct tgaaggaaat gagttatcta tccgtctcta tctttacttt
     8581 tttctttcag atacatttgg aggtaacgat tggccatttt cctcacttga attaaatgca
     8641 ctaaaaatga acagtacaga tgagcaaata gaaaaaattt caaaacaaga aaaaaatatc
     8701 tacttgctgt taactttatt catattacga aacaaacaac aggcatattt tccaaaaact
     8761 tcttctgaaa tcactgaatt gatggactta ttgcagcata cacatgatta ttcagaatac
     8821 tttacttctt tttcagtgag tcgcccttgt gaagagacag aaacagagag agcctacttt
     8881 aattattttt tctattgttt ttttccaaat caaatctctt ttcgacaaaa acaggagatt
     8941 ggttttttat tttccacaag caaaaacctt atttgtcgac aagtcggtca attgatgact
     9001 tacttaactc aacagtttcc tctcaaccat ttagaaaata aaataccaat ttatttttat
     9061 tttcttgtgt tggcagttgt ttttttcgaa ctgttgggtg aatccagcag tagtttttct
     9121 acactttgct ttccagaagc cacgtccaat cattctagtt cttttataca ggaaaagaaa
     9181 ttaagagaaa aaatcgatac aattttttca aaaaatgggg aaagaaacca aatcagtcaa
     9241 taccttagtc aattgatcca acacttacta caaacagaaa cgaaagagca gttactcatc
     9301 catttgcaga taaccaaaca gtttacagga aattatcatc ttcaaagcca tttgcagcag
     9361 ttatttcgca aagatgctgt taaaattacc gcatattcag aacatgctga tttgatcatt
     9421 acagatcagc atgagaaaat ttcagatgaa acggagactt tctatctgga tacaacgcac
     9481 aatacagtgt tatttggcaa gttaataaaa gcggttcatg aactgtatat gaaaaaaggt
     9541 tatgagaaca aaacaaagag gatgtgacaa aattcttgtc acatcctctt att
//
LOCUS       DAZKLM010000097         9467 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3764
            SAMEA115706913-rid22582563.denovo.101, whole genome shotgun
            sequence.
ACCESSION   DAZKLM010000097 DAZKLM010000000
VERSION     DAZKLM010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706913
            Sequence Read Archive: ERR13196647
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9467)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9467)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287823.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287823.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,004
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,849
            CDSs (with protein)               :: 2,849
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 41 of 96
            Pseudo Genes (incomplete)         :: 52 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 15 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9467
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706913-rid22582563.denovo.101"
                     /isolate="VRE3764"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            2..74
                     /locus_tag="ACG842_002314"
     tRNA            2..74
                     /locus_tag="ACG842_002314"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:35..37,aa:Asp,seq:gtc)
     gene            78..150
                     /locus_tag="ACG842_002315"
     tRNA            78..150
                     /locus_tag="ACG842_002315"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:111..113,aa:Phe,seq:gaa)
     gene            155..225
                     /locus_tag="ACG842_002316"
     tRNA            155..225
                     /locus_tag="ACG842_002316"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:187..189,aa:Gly,seq:tcc)
     gene            238..311
                     /locus_tag="ACG842_002317"
     tRNA            238..311
                     /locus_tag="ACG842_002317"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:272..274,aa:Ile,seq:gat)
     gene            323..394
                     /locus_tag="ACG842_002318"
     tRNA            323..394
                     /locus_tag="ACG842_002318"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:356..358,aa:Glu,seq:ttc)
     regulatory      583..662
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01767"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="SMK box riboswitch (SAM-III) riboswitch; SAM
                     riboswitches modulate the expression of downstream genes
                     in response to levels of S-adenosylmethionine; SMK box
                     riboswitch shares no known similarity to SAM-I and SAM-II;
                     Derived by automated computational analysis using gene
                     prediction method: cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF01767"
     gene            660..1853
                     /gene="metK"
                     /locus_tag="ACG842_002319"
     CDS             660..1853
                     /gene="metK"
                     /locus_tag="ACG842_002319"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HFQ0248829.1"
                     /translation="MVERHLFTSESVSEGHPDKIADQISDAILDAILKQDPTARVACE
                     TSVTTGLVLVFGEISTTAYVDIQKVVRETIKEIGYTRAKFGFDGDTAAVLVAIDEQSP
                     DIAQGVDEALEIRDEDKKDVLDEIGAGDQGLMFGFAVDETPELMPLPIALSHRLVRRL
                     ADLRKSNELTYLRPDAKSQVTVEYDDQGQPERVDTIVISTQHDDAVDNETIRHDVIEK
                     VVKEVIPAELLDDQTKYYINPTGRFVIGGPQGDAGLTGRKIIVDTYGGYARHGGGAFS
                     GKDATKVDRSASYAARYIAKNIVAAGLARKAEVQLAYAIGVAQPVSISINTFGTGTVP
                     EEELIAAVRENFDLRPAGIIEMLDLRRPIYKQTAAYGHFGRTDVDLPWEHTDKVDALK
                     ASLAK"
     gene            1983..3455
                     /locus_tag="ACG842_002320"
     CDS             1983..3455
                     /locus_tag="ACG842_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family MFS transporter"
                     /protein_id="HFQ0248830.1"
                     /translation="MKKETNVTLVTVSIFIATFMTAIEGTIVTTAMPTIVGSLHGMEI
                     MNWVFSIYLLTNAMLTPIYGKLADKVGRKPIFIFGTLLFIIGSLFCGLSQSMIGLIAA
                     RAIQGMGAGAMMPVALTIIGDLYSIEKRAKVLGLNSSAWGIASVFGPLAGGFIVETIS
                     WHWIFFINVPIGILLIILIWLYLNEPKIIHESKPMDIFGSFSLMAMLVSLLLGFQLLG
                     DEGMSPLVSALLLGSIVMLGIFVFAEKRAKDPVIMMHLFKNPLFVVVNLAAALASGFL
                     MGIDVYIPMWMQGVLGLNAAIGGLVLAPLSLVWMVGSFLASKWMTQMKTHWVLRIGLL
                     IAAIGGAWLYLMPFRSNYLWFFAISTVIGIGLGVTMTTSTVSAQNSVSRDQLGVATSF
                     NTLVRTIGQTVMVSIFGLLINGVTKRELAAANLTNDADIMNKLVNPQTAKYIDADILL
                     PLRKILYDGLHSVYLVGLLLVIIALVLTLFVHEKSGKIKQ"
     gene            3538..4326
                     /locus_tag="ACG842_002321"
     CDS             3538..4326
                     /locus_tag="ACG842_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFQ0248831.1"
                     /translation="MAFIQANIYSNVLEMEVNLNVILPQTTKKVIGTSSEQETTDIPV
                     LYLLHGMGGNHSVWERRTSIERYVANYGLAVIMPSTDLGWYTDTVYDMKYWTFIAEEL
                     PEICHELFPQLTRKREKTYAAGLSMGGYGALKLGLAKSENFAAVISLSGAVSVGNRMD
                     DLLMVRKASFWEGIFGPLDQIEGSKNDPQYLLKELVKSGKPVPRFYMACGESDFLYQA
                     NQEMVSAMQEQGLAVTFEQGPGDHNWIFWDQWIQRALAWLMEEK"
     gene            complement(4393..5061)
                     /locus_tag="ACG842_002322"
     CDS             complement(4393..5061)
                     /locus_tag="ACG842_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator
                     C-terminal domain-containing protein"
                     /protein_id="HFQ0248832.1"
                     /translation="MEIKLSQAIIIQAAFDILAETQALSQLSMRNIANRIHVQAPALY
                     WYFKNKQQLLQIMAETMEEELALPDPTLPWNEQLLQYMENYYDLYTRFPCGAELEINT
                     VPAFPSRLEHLEQMIQLLVREGFSVTQSHQAIASLHNLLIGQLMDQQHEQQLRQKIMD
                     GNEFLKETVLFMRNYVVENQLEGFKESMQSHAELNEKALFLTSAQTYIEGLCQQNKRR
                     ENEQ"
     gene            5176..6888
                     /locus_tag="ACG842_002323"
     CDS             5176..6888
                     /locus_tag="ACG842_002323"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0248833.1"
                     /translation="MKLILRHAKKYKVAVFISLLSVAVMVAAALWQPKLLQQVLEAII
                     TEDNDEMKTIGIYLISLALLGLAAGVTNTIFSAKVAQGVSADIREEAFRKIQTFSFGN
                     IEQFSAGNLVVRLTNDITQIQNLVMISLQSLFRIPFLFIGAFILAMITMPQLWWIIVA
                     LIVTVFLITALSFTRMGKHFMIIQKLIDRVNSIAKENLLGIRVVKSFVQEENQLKSFS
                     KVSEDLTKHNIIVGTLFSVMIPSFMLAANLAVVGAIFFVSDLVKDDPTLIGGIASFMN
                     YLMQIMMAIIIGGMMMMMTSRAAVSLKRIAEILDAEPDLTYLDVPEQELTGSVTFDHV
                     SFRYPGEDTDTLKDISFSIKPGEMVGIVGATGAGKSTLAQLIPRLFDPTEGKVEVGGV
                     DLRQVNEKSLRQTVSFVLQKAILFSGTIAQNLRQGKKNATEKEMEHASSIAQAKEFIE
                     KLSDRYEAPIEERSSNFSGGQKQRLSITRGVIGNPKILILDDSTSALDARSEKLVREA
                     LDRDLKGTTTIVIAQKIASVVKADRILVLDEGRLVGEGTHEELVAGNRVYQEIFETQK
                     GTEE"
     gene            6891..8663
                     /locus_tag="ACG842_002324"
     CDS             6891..8663
                     /locus_tag="ACG842_002324"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0248834.1"
                     /translation="MTDLIKASKFFYHYLKRYKLSFFFIFITVIIATYLQVKAPQYIG
                     EAFQELANYVGGLMQGVDDKSKFVSVIWKLLLFYVLASAANFIYSILFTQVVGKSTNR
                     MRIGLFNKLEKLTIRFFDSHQDGEILSRFTSDLDNIQNSLNQALLQVITNAVLLVGIL
                     IMMFRQNVELAWATIASTPVAVLIAVLIIRKARKYVDLQQDEVGKLNGYMDEKISGQR
                     VIITNGLQEETIEGFLEHNQSVREATFKGQAYSGLLFPMMQGMSLVNTAIVIFFGGWL
                     ALNGDLERSVALGLVVTFVQYSQQYYQPLMQISSGYSMIQLAITGARRLNEIFDEKDE
                     VKPENCKAFDGVEKGLALQHVDFGYSPEKLVLKDVSINVDKGEMVALVGPTGSGKTTI
                     MNLLNRFYDVNDGSVVIDGIDIRDYDLDALRRHVGIVLQESVLFSGTIKDNITFGKPD
                     ASDEEVINAAKQANIHDFIMTLEKGYDTEISEENNLFSTGQKQLISIARTIITNPSLL
                     ILDEATSNVDTVTEARIQKAMDEAIKGRTSFVIAHRLKTILNADRIVVLRDGEVIEEG
                     SHHELLAENGFYAELYHNQFVFES"
     gene            complement(8745..>9467)
                     /locus_tag="ACG842_002325"
     CDS             complement(8745..>9467)
                     /locus_tag="ACG842_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFQ0248835.1"
                     /translation="LKTFTGMIFKFANRRYGLKYNPVNGVSLARKPKTNEEIKKEKDG
                     YLSKEEVQMIASKQKSSHQQSRYSLITKFLFLTGLRYGELISLTESDYDGNKITVSGT
                     YDYELKIKTTTKNTGSYRTIELSSNAKEIIDQLIEENKLIKNNKDKYIFISKNGNPIS
                     IQAYNQSLRAVSKELNLDKKVSSHMLRHSHISLLTELGIPLKAIMDRVGHEDSKTTLK
                     IYTHTTKNMQNQLVEKLGKIEI"
BASE COUNT         2845 a         1585 c         2047 g         2990 t
ORIGIN      
        1 tggttccgtg gtgtaggggt taacatgcct gcctgtcacg caggagatcg cgggttcgat
       61 tcccgtcgga accgtttggc tcggtagctc agttggtaga gcaatggatt gaagctccat
      121 gtgtcggcag ttcgattctg tcccgcgcca ttatgcgggt gtagtttagt ggtaaaacca
      181 cagccttcca agctgttgtc gcgagttcga ttctcgtcac ccgcttttag tttttatggg
      241 cctatagctc agctggttag agcgcacgcc tgataagcgt gaggtcgatg gttcgagtcc
      301 atttaggccc atttatttta ttggcccgtt ggtcaagcgg ttaagacacc gccctttcac
      361 ggcggtaaca cgggttcgaa tcccgtacgg gtcatggaag atactttttg agtatctttt
      421 tctttttatc tgaaagaatg attttaaaaa gcttagcaga gttatcttta tggatgattg
      481 tgctaagctt ttttggacag ttctaatgta aaatattttt atatttttta agcaaaaaaa
      541 tgaaaaaata cgagcagaaa actgttttat ctgctatact tggttcgttg caagttcccg
      601 aaaggatccg caggacggag atgccttgta accgaattta actaggggga atcattataa
      661 tggtagaaag acacttattt acatcagaat ctgtttctga aggacatcca gataaaattg
      721 ctgaccaaat cagtgatgca atcttggatg caattttaaa acaagatcca acagcacgag
      781 tagcgtgtga aacgtctgta acaactggtc ttgttttagt atttggagaa atatctacga
      841 cggcatatgt agatattcaa aaagtcgtac gggaaacaat aaaagaaatt ggttatacac
      901 gtgcaaaatt cggatttgat ggagatacag cagctgtatt ggttgcgatt gatgaacaat
      961 ctcctgatat tgctcaagga gttgatgaag cacttgagat ccgggatgaa gataaaaaag
     1021 acgtattaga tgagattggt gctggtgacc aaggtttaat gtttgggttt gctgtagatg
     1081 aaacaccaga attgatgcct ttaccaatcg ctttgagtca tcgtctggta cgacgcttgg
     1141 cagatttgcg taaatcaaat gaattaacgt atttacgtcc agatgcaaaa tctcaagtaa
     1201 cggttgaata tgatgatcaa ggacaaccgg aacgcgtaga tacaatcgtt atttcaacac
     1261 agcatgatga tgcagtagat aatgaaacaa ttcgtcatga tgtcatcgaa aaagtagtga
     1321 aggaagttat tccagctgaa ttattagatg atcaaacgaa atattatatc aatccgactg
     1381 gccgatttgt cattggtggt cctcaagggg atgccggatt aacaggaaga aaaatcattg
     1441 ttgatacgta tggcggttat gctcgtcatg gtggcggtgc tttttctggt aaagatgcga
     1501 caaaagttga ccgttctgcc agctatgctg cacgttatat tgctaaaaac attgttgcag
     1561 cagggcttgc tcgcaaagca gaagtacagc tagcttatgc gattggtgtt gctcaacctg
     1621 tttcgatctc aatcaatact ttcggtactg gaacggttcc ggaagaagaa ctgattgctg
     1681 cagtgagaga aaacttcgat cttcggccag caggaatcat cgagatgctt gatttgcgtc
     1741 gtccgattta caaacaaaca gctgcttacg gtcattttgg tcgtacagat gtagatttgc
     1801 cttgggaaca tacggacaaa gtagatgcgt tgaaagcaag tttagcaaaa taagttatat
     1861 tcagctggaa cgatcgccaa gtggtggttg ttccagcttt ttttatggca gttaacctct
     1921 tgaaaaagat agtaattatt tatctatttt tagatcctgt cataatgaaa gggaggaata
     1981 agatgaaaaa agaaacaaat gttactttgg tcacagtgag tattttcatt gcaacgttca
     2041 tgacggctat tgaaggtaca atcgtaacaa ccgctatgcc aacgattgtc ggttcattac
     2101 atggaatgga aatcatgaac tgggtatttt cgatttattt gctgacaaac gctatgctta
     2161 ctccgattta cggaaaactt gcggataaag tgggacggaa accaatcttt atttttggga
     2221 cattattgtt tattatcggt tcgttatttt gcggtttatc tcaaagcatg attgggttga
     2281 ttgctgctcg tgcgattcaa gggatgggtg ctggggcaat gatgccagta gctttgacga
     2341 tcatcggcga tctttattct attgaaaagc gagcaaaagt tttaggtttg aatagttcag
     2401 cctgggggat tgccagtgtt tttggaccgc ttgcgggagg attcatcgtt gaaacgatca
     2461 gctggcactg gatctttttt atcaatgtgc cgatcggaat tctattaatt attttgattt
     2521 ggctatattt aaatgaacca aaaatcatac atgagtctaa accaatggat atttttggta
     2581 gttttagttt gatggccatg cttgtctcat tgctgttagg gttccaatta ttaggagacg
     2641 aaggaatgag tccgcttgtt tctgctttgc tgctaggtag tatcgtaatg ttaggtattt
     2701 ttgtttttgc cgaaaaaaga gctaaagatc ctgtcatcat gatgcattta ttcaaaaatc
     2761 cactatttgt tgtggtcaac ttggctgcag cattggctag tggttttttg atgggaatcg
     2821 atgtttatat ccctatgtgg atgcaaggtg tattagggct gaatgcagca attggtgggc
     2881 tggtattagc accattatca ctagtctgga tggtcggttc tttccttgca agcaagtgga
     2941 tgactcaaat gaaaactcat tgggtcttga gaattggact actaatcgca gctattgggg
     3001 gcgcttggtt gtatcttatg cctttcagaa gtaactattt atggtttttt gctatatcaa
     3061 cggtcatcgg tataggctta ggtgtgacca tgactacctc gactgtcagt gcacaaaata
     3121 gtgtatctcg tgaccagctt ggtgtagcaa cttcttttaa tacgttagtt cgaacaattg
     3181 gtcaaacagt catggtttct atttttggtt tattgataaa tggggtaact aaacgagaac
     3241 ttgcagcagc aaatttaaca aatgatgcag atatcatgaa taaactggtg aatccacaaa
     3301 cggctaaata tattgatgcg gatattttgt taccattaag aaaaattctt tatgatgggt
     3361 tacattccgt ttatctagtt ggcttgttac tcgtgatcat tgctttggtc ttaacgcttt
     3421 tcgttcatga aaaaagtggt aaaataaaac agtagtttcg gttaagatat gtacgtagcc
     3481 gaatcattca aatttaaagt tattcaaaaa taacttctta ctaaggaagg cgaaaatatg
     3541 gcatttattc aagcaaatat ttattcgaat gtgttagaaa tggaagtcaa tctaaatgtg
     3601 atactaccgc aaacaacaaa aaaggtgatt ggcaccagtt cagagcaaga gacaacggat
     3661 atacctgttt tatatttact tcatggaatg ggtggcaatc atagtgtatg ggaaagacgt
     3721 acgtcgattg aacgttatgt agcaaactac ggattagctg tcatcatgcc atctactgat
     3781 cttggctggt acacagatac agtttatgac atgaagtatt ggacattcat tgcagaagag
     3841 ctacctgaga tttgtcatga attattcccg caattaacaa gaaaaagaga aaaaacatat
     3901 gccgctggat tatcgatggg cggatatggc gcgttaaaac taggccttgc aaaatcagaa
     3961 aatttcgcag cagtaatctc tctttcaggc gctgtttctg taggaaatcg aatggatgat
     4021 cttttgatgg ttcgtaaagc atctttttgg gaagggatct ttggaccatt ggatcaaatc
     4081 gaaggttcaa aaaatgatcc gcaatactta ttgaaagaac tagtaaaaag cggaaaacca
     4141 gttccaagat tttatatggc ttgtggggaa tctgattttc tttatcaggc aaatcaggaa
     4201 atggtatctg ctatgcagga acaaggcttg gcggtcacct ttgaacaagg acctggcgat
     4261 cataactgga tcttctggga ccaatggatt caacgtgcat tggcgtggct aatggaagaa
     4321 aaataaaaaa agagtcttaa aaagatagct cctatagaga tcacaaacat agtgatctct
     4381 ataggagctt ttttactgct cgttttctct tcttttattt tgttggcata agccttcaat
     4441 ataggtttgt gcactcgtta gaaagagtgc tttttcattc aattctgcat gggactgcat
     4501 actttcttta aatccctcta attgattttc aactacataa ttacgcataa atagaactgt
     4561 ttctttcaaa aattcatttc cgtccataat tttttgtcga agctgctgtt catgctgctg
     4621 atccattaac tgtccaatca ataaattgtg gagagaggca atggcctgat gactttgggt
     4681 gactgaaaat ccttcacgaa caagtaattg gatcatttgc tccaaatgtt ctaatcgact
     4741 cggaaaagcg ggcacagtat tgatttctaa ttctgcccca caagggaaac gtgtataaag
     4801 gtcatagtaa ttttccatat actgcagcaa ctgttcattc caaggcaaag ttggatctgg
     4861 aagcgctaat tcttcttcca ttgtttcagc cattatttgc aagagctgtt gtttgttttt
     4921 gaaataccaa tataaagcag gtgcttgaac atgaattctg tttgcgatgt ttcgcatgga
     4981 aagctgcgaa agagcttgtg tttcagccag gatatcaaag gcggcctgaa tgatgattgc
     5041 ttgagaaagc tttatttcca ttttatcaac ctcgtgtatt gcattatctt tttaaacatg
     5101 gtaaattgtt agtcggtcta attttacagt gttaaattag tcctgtcaat ttaaacaaag
     5161 gggtgtaatc aacgaatgaa attaatctta cgacatgcga aaaaatataa agtagctgtt
     5221 tttatttcct tgctgtccgt agcagtcatg gttgcagcag cattatggca acctaagtta
     5281 ctacagcaag tattggaagc tattattaca gaagataatg atgaaatgaa aacaattggt
     5341 atttacctga tcagtctggc tttgctagga ttagccgcag gtgtaacgaa cacgattttc
     5401 tctgcaaaag tagctcaagg cgtaagtgca gatatccgag aagaagcatt tcgaaagatc
     5461 cagacatttt catttggaaa tatcgaacag ttctctgctg gaaatcttgt tgttcgtcta
     5521 acaaatgata tcacgcaaat ccaaaattta gtgatgattt ctttgcaatc actttttcga
     5581 attccattct tatttattgg tgccttcatt ttagctatga tcacgatgcc gcaattatgg
     5641 tggatcatcg tagcgcttat cgttacggta tttctgatta cggcactttc atttacaagg
     5701 atgggcaagc acttcatgat cattcaaaaa ctgattgatc gtgtgaatag cattgcaaaa
     5761 gaaaacttac tagggattcg tgtagtgaaa tcttttgtac aagaagaaaa ccaattgaag
     5821 tcattcagca aagtaagtga ggacttaaca aaacacaaca tcatagtcgg tacattattt
     5881 tctgtgatga tcccgtcctt catgctggct gccaatttgg ctgtggttgg ggcgatcttt
     5941 tttgtgagcg atcttgttaa agatgatcct acgttaattg gtggaattgc ttcattcatg
     6001 aactacttga tgcagattat gatggcaatc attattggcg gaatgatgat gatgatgact
     6061 tcaagagcag ccgtatcgct gaagcggatt gcggaaattt tagatgctga accagatctt
     6121 acttatttag acgttccaga acaagagttg acgggttctg tcacttttga ccatgtttct
     6181 tttcgttatc ctggtgagga tacagatacg cttaaagata tcagcttttc tatcaaacct
     6241 ggagaaatgg ttggtatcgt cggggcgact ggtgccggga agtcaacact agcacaattg
     6301 atccctcgtc tatttgatcc gacagaaggc aaagttgaag taggtggtgt agatcttcgt
     6361 caagtaaatg aaaagagttt aagacaaacg gtttcctttg ttttgcaaaa agcaatttta
     6421 ttctcaggaa cgattgctca aaacttgcgc caagggaaaa agaatgcaac agaaaaagag
     6481 atggaacatg cttcttctat tgctcaagca aaagaattca tcgaaaagtt atctgatcgt
     6541 tacgaagcac caatagaaga acgtagcagt aacttttctg gtggtcaaaa gcaacgttta
     6601 tccattactc gtggcgttat cggtaatcca aagatcttga tcctagatga cagtacgagt
     6661 gcactggatg ctcgctctga aaagctggtc cgagaagcac tcgatcgaga tttgaaggga
     6721 acaacgacga ttgtcatcgc acaaaaaatt gcttctgttg tcaaagcaga ccgcatctta
     6781 gtattggatg aaggcagatt agtcggcgaa ggaacccatg aggaattggt agctggaaac
     6841 cgtgtctatc aggaaatctt tgaaactcaa aaaggaacgg aggagtaagt atgactgatc
     6901 taataaaagc aagtaaattt ttctatcact atttgaagcg gtacaaactc tcctttttct
     6961 ttatctttat cacagtgatc attgctacct atctacaagt aaaggcacct caatacatcg
     7021 gggaagcttt tcaagaatta gctaattacg taggaggctt gatgcaaggc gtagacgata
     7081 aaagcaaatt tgtttctgta atatggaaac tcttgctgtt ttatgtacta gctagtgcag
     7141 ccaactttat ctatagtatt ttgttcacac aagtagtagg gaagtcaacg aaccgaatgc
     7201 gtatcggtct attcaacaaa ttggaaaaat tgacgatccg tttctttgat tctcatcaag
     7261 acggtgaaat tttaagtcgt tttaccagtg acttagataa tattcaaaat agtttgaacc
     7321 aagctttgct tcaagtcatc acgaatgctg ttttacttgt cgggatcttg atcatgatgt
     7381 tccgccaaaa tgtagagtta gcttgggcta caattgcttc cactccagta gccgttttga
     7441 tagctgtctt gattatccgc aaagcacgga aatatgtgga tcttcagcaa gatgaagtgg
     7501 ggaaactaaa tggttatatg gatgaaaaaa tcagcggaca acgtgtcatc atcacaaatg
     7561 gcttacaaga agaaacgatt gaaggattct tagagcataa tcaaagtgtt cgcgaagcaa
     7621 ccttcaaagg acaagcttat tctggtctac tatttccgat gatgcaagga atgtcactgg
     7681 tcaatacagc gatagttatc tttttcggcg gctggttagc attaaatggt gatttggaac
     7741 gttcggtggc attaggctta gttgtgacat tcgtccagta ttcacaacaa tattaccaac
     7801 cactgatgca gatttcatct ggatacagca tgatccaatt agcgattaca ggggcaagac
     7861 gattgaatga aatatttgat gaaaaagatg aagtcaaacc agaaaactgt aaagcatttg
     7921 atggtgttga aaaaggtctt gcgttgcagc acgtagattt tggttattct ccagaaaaac
     7981 tagtcttgaa agatgtctcg atcaatgtgg acaaaggaga aatggttgct cttgttggtc
     8041 caacaggatc ggggaaaacg acgatcatga atctgctaaa ccgtttttac gatgtcaatg
     8101 atggttcagt agtcatcgat ggaatagata tccgagatta tgatcttgat gcattacgta
     8161 gacatgtagg aatcgttttg caagaatctg tattgttctc cggaacgatc aaagacaata
     8221 tcacattcgg caaaccggac gcttcagatg aagaagtcat caatgcagca aaacaagcga
     8281 atattcatga cttcatcatg acgttagaaa aaggttatga tacagagata tccgaagaaa
     8341 ataacttatt cagtacaggt caaaagcagt tgatcagtat tgctcgaacg atcatcacaa
     8401 atccatcttt attgattttg gatgaagcaa caagtaatgt agacacggta acagaagcaa
     8461 ggatccaaaa agcaatggat gaagcaatca aaggaagaac cagctttgtg attgctcacc
     8521 ggctgaaaac aatcttgaat gcagatagaa tcgttgtctt gcgtgatggg gaagtcattg
     8581 aagaaggcag tcatcacgag ttattagcag aaaatggatt ttatgcggaa ctttatcata
     8641 atcaatttgt atttgaaagt taaattaaaa gaacctcgtt ataacggggt tctttttata
     8701 tattttatgt tgtttgtttt tataaggggc agaaaagggg caatttaaat ttctattttt
     8761 cctagttttt ctactagttg attttgcata tttttagttg tatgagtata aatctttaat
     8821 gttgtttttg aatcttcatg tcctacacga tccataatgg cttttaatgg gattcctaat
     8881 tctgtaagta gagaaatgtg ggaatgcctt aacatgtgag aagaaacttt tttatctagg
     8941 tttaattctt tggatactgc tcttaaagat tgattatatg cttgaataga tatcgggtta
     9001 ccatttttag agatgaaaat gtatttatct ttattattct taattaattt gttttcttca
     9061 attaattgat ctatgatttc tttagcatta cttgataatt caatggttct ataagaacct
     9121 gtatttttag ttgttgtttt aatttttaac tcgtaatcat aagtaccaga aaccgtaatt
     9181 ttattaccat cataatcaga ttcagtcaaa gatatcaatt caccataacg tagtccagtt
     9241 aaaaacaaaa atttagtaat taaagaataa cgtgactgtt gatgtgaact tttttgttta
     9301 ctagcaatca tttgaacttc ttcttttgat aaataaccat ctttttcttt ttttatttct
     9361 tcgtttgtct taggttttct tgctaaagaa accccattta cgggattata ttttaatcca
     9421 tatcgacggt tggcgaattt aaaaatcatt cctgtaaaag tttttaa
//
LOCUS       DAZKLM010000098         9437 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3764
            SAMEA115706913-rid22582563.denovo.102, whole genome shotgun
            sequence.
ACCESSION   DAZKLM010000098 DAZKLM010000000
VERSION     DAZKLM010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706913
            Sequence Read Archive: ERR13196647
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9437)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9437)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287823.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287823.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,004
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,849
            CDSs (with protein)               :: 2,849
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 41 of 96
            Pseudo Genes (incomplete)         :: 52 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 15 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9437
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706913-rid22582563.denovo.102"
                     /isolate="VRE3764"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            52..246
                     /locus_tag="ACG842_002326"
     CDS             52..246
                     /locus_tag="ACG842_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0248836.1"
                     /translation="MYYVEVKTKGVKNKQYVKGISNEYPLLGSWKEAAPFSKPCAIKI
                     KNELEKELTCGKAVVDIIEK"
     gene            515..814
                     /locus_tag="ACG842_002327"
     CDS             515..814
                     /locus_tag="ACG842_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0248837.1"
                     /translation="MDYTVEFDSELLKQFPQEEHFMYKEAISHKVTEYLEHNFFRIKP
                     VRTRSAKGIYEMKVKINKKNYRIAFALKKNHVFIFYISSNLQKIVFDKEVSKKIK"
     gene            complement(911..1345)
                     /locus_tag="ACG842_002328"
     CDS             complement(911..1345)
                     /locus_tag="ACG842_002328"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0248838.1"
                     /translation="MNIDSLMIDEISELYKSVGWTNYTKDTARLARAFEQSESLIKRN
                     GEGKIIGIVRWITDFATIAFIQDILIHPRYQRQGIGKVLLNEALEKITSYGPVQIELL
                     TDDTEKTKKFYESVGFVQVKEMGGISYVIDTRRKKVLFGNDK"
     gene            complement(1577..2287)
                     /locus_tag="ACG842_002329"
     CDS             complement(1577..2287)
                     /locus_tag="ACG842_002329"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008742 - L-ribulose-phosphate 4-epimerase
                     activity [Evidence IEA];
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019569 - L-arabinose catabolic process to
                     xylulose 5-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HFQ0248839.1"
                     /translation="MSCEEKIAAMKQRVLEANLALPRYGLVKLTWGNVSEVDRELGVI
                     VIKPSGVRYECMQADQMVVTDLSGNIIEEDSLKPSSDLPTHVVLYQTFEDITAITHTH
                     STHSVMWAQAGRDLPAYGTTHADAFYGKVPCTRQLTKEEVREAYEVHTGNVIVETFKE
                     RKLDPNEVPGVLVYGHGPFTWGDSPMKAVENSLILDEICLMAKENELINPNICEIPQY
                     LLDKHYFRKHGKYAYYGQ"
     gene            complement(2277..3137)
                     /locus_tag="ACG842_002330"
     CDS             complement(2277..3137)
                     /locus_tag="ACG842_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016861 - intramolecular oxidoreductase
                     activity, interconverting aldoses and ketoses [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HFQ0248840.1"
                     /translation="MAQIGIYEKALPKDISWKERFSLVKEMDFDFIEMSIDETDERLA
                     RLDWSEEKIAELREEMFSSGVRIHSICLSAHRRFPFGSADPEKKKAAKQLMKKAIHLA
                     HNLSVKVIQIAGYDVYYEEKSMNSREDFIEGIKESVKEASKYGIILSVEIMDDPFMNS
                     ISKFLEIKEQIHSPFLQVYPDLGNLSAWPENNPARELEKGIDYITAIHLKDTYPVTKE
                     YSGQFRDVPFGSGCVDFLGLLRHLKRLDYDGTFLIEMWSEKSQDFRREIQQAKDYLSS
                     KLKEAGYDVL"
     gene            complement(3142..3780)
                     /locus_tag="ACG842_002331"
     CDS             complement(3142..3780)
                     /locus_tag="ACG842_002331"
                     /EC_number="4.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HFQ0248841.1"
                     /translation="MSRPNLQIALDHNDLEHALGDVMKVGDVVDIIEVGTILCLQEGQ
                     KAIRCIRSMFPDKKLVADTKCADAGGTVASNVAKAGADWMTVICCATIPTMEAAQKEI
                     GELQVELYGNWTFEQAMDWHNIGIRQVIYHQSRDALLSGETWGEKDLSKIKKLIELGF
                     NVSVTGGLNPHTLHLFEGIDVYTFITGRGITAANDPMKAAQNFKDEIIRIWG"
     gene            complement(3795..4094)
                     /locus_tag="ACG842_002332"
     CDS             complement(3795..4094)
                     /locus_tag="ACG842_002332"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFQ0248842.1"
                     /translation="MRILVSCANGSGTSLMMMRSVEKAMKELNVPITKIHHCAISEGK
                     SSAGQYDVVFTPVNFLNMFTQAEKKGVTVIGIKNVMSVKEISEKFAETKWYQKNK"
     gene            complement(4137..5621)
                     /locus_tag="ACG842_002333"
     CDS             complement(4137..5621)
                     /locus_tag="ACG842_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HFQ0248843.1"
                     /translation="MGEFLLSIWTYFATNILTQPAYLIGFIVLLGYILLKRPLHECIA
                     GFLKATVGYFILTVGSGGLVNNFRPILVGLKERFNLDAMVTDPYFGQNAVDAGLMETF
                     GRTFGDVMILLLIAFVLNILLVRFQKYTKLRSVFTTGNVQVQQAATAFWILLFCFPDL
                     GRIQALIFMGLILGCYWTVASNLTVGITQELTEGAGFAVAHQQMFGIFIFAKLAEWMK
                     KRDEKKNRSIKQDKKIEDIKLPGFLSIFNENMVATSLLMLFFFGIILIVLGKDYLIQA
                     QFMQEGQSFLFYIMTTSLNFAVYLAILQLGVRTFVDELTQSFQGISNTILPGAVPGID
                     VAATFGFGSPNAVTIGVLFGALGQFLMIGLLILLKSPTIVIAGFIPLFFDNAVIAVYA
                     NNRGGFKAACLFPFLSGIIQVGGSALFATWIGMSTYGGYIGMFDWATAWPLFTVIMKV
                     LGYAGIAVVVIGLLAIPQLQYRKNPDGYFLIVEDYDTYAEKQRV"
     gene            complement(5641..6102)
                     /locus_tag="ACG842_002334"
     CDS             complement(5641..6102)
                     /locus_tag="ACG842_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFQ0248844.1"
                     /translation="MLKYFFDHQLIHYPDKEITDWREAIAESCLVLIDKEIIDETYVE
                     EIIHCVEKYGPYIVIAPQVAMPHSSEKSDGVFGTAISFTKLKQPIRFESEGENEEKTA
                     SLFFTLAAKDPEEHLENIQKLSELLMTDGLVERLISTNSIDDYQVVMAEYN"
     gene            complement(6120..7187)
                     /gene="ulaG"
                     /locus_tag="ACG842_002335"
     CDS             complement(6120..7187)
                     /gene="ulaG"
                     /locus_tag="ACG842_002335"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035460 - L-ascorbate 6-phosphate
                     lactonase activity [Evidence IEA];
                     GO_process: GO:0019854 - L-ascorbic acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HFQ0248845.1"
                     /translation="MSKTVNEVTKEKWLVETFPEWGTYLNEEIETEVVDPGTFAMWWL
                     GCTGIWLKSHEGTNILCDLWCGTGKRSHGNGMMKKGHQMMRMSGVQNMQPNLRTQPFV
                     IDPFAIKNVDALIVTHIHSDHLDIHTAAAVHQNCPEATFIGPKEVVRTWQKWGIPEEK
                     TITVKPGDAVKIKDINVVALEAFDRTALVTCEDPEEVLKGKMPQEMDQIAVNYLFETS
                     GGNLYHAGDSHYSNLFAKHGNEHKIDVCLGAYGENPRGITDKVTSVDMLRMAESLNAK
                     VVIPVHYDIWSNFMADPKEITEIWKFKKDRLKYGFKPYIWQVGGKFIYPTNKDDLEFN
                     FYRGFEDVFSIENDTPFPSFL"
     gene            7405..8175
                     /locus_tag="ACG842_002336"
     CDS             7405..8175
                     /locus_tag="ACG842_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFQ0248846.1"
                     /translation="MKNSLVNIEKRQKEILELLQQTEQLSTFELAESLDVSISTIRRD
                     LNLLEEKNEIIRNYGYCSYNYKNQTDFDQSGPERIKQAIAREASKYINDYDTLFINSS
                     STALKTLDFLQSNHLTVITNNVKINYSPHKDNCSYILTGGEMRFPKEALVGDIALNTI
                     SSVNADICIIGCSGVDVVHGVTTKILNESTINEMMVNKTIKCKILVADHRKIGLTSKF
                     KIADISVFDYLITDKFCSEKVLEEIRNTGIKTIQVDSF"
     gene            complement(8448..9275)
                     /locus_tag="ACG842_002337"
     CDS             complement(8448..9275)
                     /locus_tag="ACG842_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0248847.1"
                     /translation="MIKVDGALLKEYRKKKGLTQVQLAEGICEQATVSNIENKNMSPG
                     INILSALCKKLNVEISDILVETEEDKIRKKINEIQSLNSVSKYQESYKILQTIDQEKV
                     SNSDLLNQILFYQGLLDHLYAKKPDAALFYFNQVLEQTTETDIHHLQAAANVAMIYLS
                     KGELDFAKVYVERTLKILSETDFDNLMVCIVYYDIATYYRKIEDYDKAIQLCEKGIEY
                     NKKHKSTYALEYLLYEIASCHKQLGEDDYLERYMDAKKIARFNGNDYAVKVIENDLK"
BASE COUNT         2932 a         1936 c         1483 g         3086 t
ORIGIN      
        1 cttacatata tttgaatatt aactaaaaac agcaagaaag aagaggatca aatgtattat
       61 gtagaagtaa aaacaaaagg cgtcaaaaac aaacaatatg tgaaaggtat cagtaacgaa
      121 tatcctttac ttggttcatg gaaagaagct gctccttttt ccaaaccatg tgccataaaa
      181 ataaagaacg agctagaaaa agaactgact tgtggtaaag ctgttgtaga tatcatcgaa
      241 aagtaaatcc aatgtataga aaggaagatc acacatgaaa agcgacgtac aaattgctaa
      301 gattatagaa ttagaagaag aagacgcagt gttgacaatt gaaaaaaaat aaatgtttgt
      361 ataccatatg attatttcaa tatgtatcct ggaattggag atctcgtaga gctatatcaa
      421 gatgaatcaa atatacttgt cattcatcat gaaatataga gacttataaa attgtttatt
      481 tttataatgt atcagtatag aaaggaaata aaacatggat tatactgttg aatttgacag
      541 tgaactactt aagcaatttc cacaagaaga acatttcatg tataaagaag caatttccca
      601 caaagtgaca gagtatttgg agcataattt ttttcgtata aagccagtgc gtactcgttc
      661 ggcaaaagga atatacgaga tgaaagtcaa aattaataaa aaaaattatc gcatagcatt
      721 tgctttaaaa aaaaatcatg tgttcatttt ttatattagt tcgaatttgc aaaaaattgt
      781 atttgataaa gaggtgagta aaaaaataaa atagctgagc attggaggat agattgagtg
      841 atactaagtc tatcctcttg tttttctaaa gaactaagct agaatttttt cttttgtacg
      901 ttattatatg ttatttgtca ttaccaaaaa gcaccttttt tcttctagta tctatgacat
      961 aagaaatacc acccatttcc tttacttgaa caaatcctac tgattcataa aatttttttg
     1021 ttttctcagt atcatctgtc aataattcga tttggactgg tccataacta gtgatcttct
     1081 ccaaagcttc attcaaaagc actttgccta ttccttggcg ctgatacctc ggatgaatga
     1141 ggatatcttg aataaatgcg atcgtcgcaa aatcagtgat ccaacgaacg attccgataa
     1201 tcttcccttc cccattacgc tttatcagag attcagattg ttcaaatgct ctagctaacc
     1261 ttgcagtgtc ttttgtgtaa ttcgtccaac caacgctctt atataattca gaaatctcat
     1321 cgatcataag ggaatcaata ttcatttttt tcttcctttc ttatacaatt ctccctctaa
     1381 taatacagtt ctttgactat ttaatctatc acatataact ctcctgtaaa gattctctcg
     1441 attccttaaa attcctcttt ccttttagaa tgcatacttt ttgataaata aaaaggttgt
     1501 gataaatttt cttcacaacc tctgtttctc tctatcaagt gttatctttt aattttctac
     1561 ccttacccta gcatctctat tgcccgtagt atgcatactt tccatgtttg cgaaaataat
     1621 gtttatctag taaatattgc gggatttcac atatattggg gttgatcagc tcgttttctt
     1681 tggccatcag acaaatttca tctaagatca aactgttttc tactgctttc ataggtgaat
     1741 ccccccatgt gaacggtcca tgtccataga caagtactcc agggacttcg tttggatcta
     1801 attttctttc cttaaacgtc tcgacaatca cattccctgt atgaacttcg taagcttctc
     1861 ttacttcttc tttcgttaat tggcgtgtac aaggaacctt accataaaaa gcatcagcat
     1921 gagtcgtgcc ataagctggc aaatcgcgtc ctgcttgcgc ccacatgacg gagtgcgtgg
     1981 aatgagtatg agtaatagca gttatatctt caaaagtttg atacaaaaca acatgtgtgg
     2041 ggagatctga agatggtttt aagctatctt cttctattat atttcccgac aaatctgtta
     2101 ccaccatttg atctgcttgc atgcattcat atctgacacc gcttggctta atgacgatga
     2161 ctcctagctc acggtccact tcactcacat tgccccaagt caatttgact aatccgtatc
     2221 gcggcaatgc cagatttgct tcaaggacac gttgcttcat tgctgcaatt ttttcttcac
     2281 aagacatcat agccagcctc cttcaattta gaacttaagt agtcttttgc ctgctggatt
     2341 tctcttcgaa agtcttggct tttttcactc cacatttcaa tcagaaatgt tccatcataa
     2401 tctagacgtt tcaaatgtct taataatcca agaaaatcaa cacaaccgct accaaaagga
     2461 acatcacgaa actggcctga atattctttt gttacagggt aagtatcttt taagtggata
     2521 gcagtgatat aatcgatccc tttctcaagt tctcgagccg gattattttc cggccatgcg
     2581 gacaaattgc ctaggtctgg atatacttgt agaaaaggtg aatgaatctg ttctttgatt
     2641 tctaaaaatt ttgaaatgga gttcataaat ggatcatcca tgatttccac ggaaagaatg
     2701 atgccatatt tagaagcttc ctttacgctt tcttttattc cttcgataaa gtcttctctg
     2761 gaattcattg atttctcttc ataataaaca tcgtatccag ctatttggat tactttgacg
     2821 cttagattat gcgccaaatg gattgctttt ttcattagtt gtttcgctgc ttttttcttt
     2881 tctggatcag cagaaccaaa cggaaatcgc cgatgcgcac ttaaacaaat cgaatggata
     2941 cgtacaccgg agctgaacat ctcttctctc agctcagcta tcttttcttc agaccaatct
     3001 aatcgtgcta accgctcatc cgtctcatca atcgacatct cgataaaatc gaagtccatt
     3061 tcttttacaa gggaaaaacg ttctttccat gaaatatctt tgggtaacgc tttttcataa
     3121 atcccaattt gagccatttc cttaccccca gatacgaatg atctcatctt tgaagttttg
     3181 tgcggccttc atcggatcat ttgctgctgt aataccacga ccagtgataa aggtatacac
     3241 atcgatccct tcaaataagt gtaatgtgtg tggatttaat ccaccagtta cagaaacatt
     3301 aaagcctagt tcaatcagtt ttttgatctt cgataaatct ttttcacccc aagtttcacc
     3361 agataacagc gcatcacggc tttgatggta gattacctgc cggataccga tgttatgcca
     3421 atccatggct tgttcaaaag tccaattgcc gtagagttct acttgcaatt cacctatttc
     3481 tttttgtgct gcctccatag ttggaatcgt tgcacaacaa atgactgtca tccagtctgc
     3541 tcctgctttt gcaacattac tcgccactgt tcctccagcg tctgcacatt tcgtatcagc
     3601 gactagtttt ttatctggga acatactgcg aatacagcga atcgcttttt gtccttcctg
     3661 taagcaaaga attgtgccca cttcaataat atctactaca tcaccaactt tcataacatc
     3721 tcctaatgca tgttctaagt cattgtgatc taacgcaatt tgcaaattcg gcctgctcat
     3781 cttagttcct tcctttactt gtttttttgg taccactttg tttcagcaaa tttttcactg
     3841 atttctttga ctgacatcac atttttgata ccgataactg taactccttt tttctctgcc
     3901 tgagtaaaca tattcaaaaa attgactggc gtaaaaacta catcgtattg gccagctgaa
     3961 ctttttccct ctgaaatggc acaatgatgg attttggtaa ttggtacgtt tagttctttc
     4021 attgcttttt ccacacttct catcatcatc aagcttgttc ctgatccatt tgcacacgat
     4081 actaaaattc tcatcttaat ccctccatta tgagtgttgt ctaatcagat tttcttttat
     4141 actctctgct tttctgcata tgtgtcatag tcttctacaa tcaaaaaata accatcagga
     4201 tttttccgat attgtaactg cggaatagcc aataaaccaa tgacaactac ggcaattcca
     4261 gcataaccta ggactttcat gattactgta aacaatggcc aagctgttgc ccagtcgaac
     4321 atcccgatat aaccaccgta tgttgacatc ccaatccatg tagcgaataa tgctgaaccg
     4381 cctacttgta tgattcctga aagaaacggg aacaggcaag cggctttgaa tccacctcgg
     4441 ttattggcat aaactgctat aacggcatta tcaaagaaca acggaatgaa accggctatg
     4501 acgatcgttg gtgatttcaa taaaatcagt aaaccaatca tcaaaaactg tcccaaagca
     4561 ccaaacaaga ccccaatcgt taccgcattt ggcgaaccaa agccaaaagt agcagctacg
     4621 tcaattcctg gaacagctcc tggtaaaatc gtgtttgaga ttccttggaa agattgagtt
     4681 agttcatcga cgaatgtccg aacacctaat tgcaagatcg ccagatatac cgcaaaattt
     4741 aaagaagtcg tcataatgta gaataagaaa ctctgccctt cttgcatgaa ttgcgcttga
     4801 atcaaataat ctttgcctag tacaatcaga atgatcccaa agaaaaatag catgagtaac
     4861 gaagtagcaa ccatattctc attgaaaatc gacaagaatc ctggtaattt gatatcttca
     4921 atttttttgt cttgcttgat tgatctattt ttcttttcat cccgcttttt catccactca
     4981 gctaattttg caaatataaa gattccgaac atttgctgat gtgccactgc aaatccggca
     5041 ccttctgtca attcctgcgt gattcctact gttaagttag aagctactgt ccaataacaa
     5101 cctaaaatca gtcccataaa aatcaatgct tgtatcctac ctagatcagg aaagcaaaac
     5161 agtaaaatcc aaaaagcggt agctgcttgt tgaacctgaa cgtttccggt agtgaaaacg
     5221 gaacgcagtt tcgtatattt ctgaaaacgg actaataaga tatttaaaac aaatgcaatc
     5281 agtaataaaa tcatcacatc tccaaaggta cggccaaatg tctccattaa tccagcatcc
     5341 acagcatttt gtccaaaata aggatctgta accatggcat ccaaattgaa ccgttccttt
     5401 agtccaacca agattggccg aaaattattg accaaaccac cagagccaac agtcaaaata
     5461 aagtaaccga ccgttgcctt taagaatcca gcgatgcatt catgaagcgg gcgtttcagc
     5521 aaaatatagc ctaataacac gataaaacca atcaggtatg ctggctgcgt caagatattt
     5581 gttgcaaaat acgtccaaat gcttaacaaa aattccccca tcttttttct cctctatcaa
     5641 ttaattatat tctgccatca caacttggta atcatcgatg gagttcgtag agatgagacg
     5701 ctcaaccaat ccatctgtca ttaataattc ggataatttt tggatatttt ctagatgttc
     5761 ttctggatct tttgctgcga gtgtaaaaaa caaactagcc gttttctcct cattctcccc
     5821 ttcactttca aaacggattg gctgtttcaa cttggtaaat gaaatagctg ttccaaaaac
     5881 accatcactt ttttctgaag aatgcggcat tgctacttga ggtgcgatca caatataagg
     5941 accgtatttt tctacacaat ggatgatctc ctccacatac gtttcatcaa taatttcctt
     6001 atcgatcaat acaagacagc tttcggcgat agcttcacgc caatccgtga tttctttatc
     6061 tgggtaatga atcaattgat ggtcaaaaaa gtatttcagc aagaaactcc ccttctttct
     6121 tataaaaacg acggaaacgg tgtatcattt tcaatcgaga agacatcttc aaatcctcga
     6181 taaaaattaa actcaagatc atctttgttt gtcggataga tgaatttccc gcctacttgc
     6241 cagatataag gtttaaatcc atacttcagg cgatcctttt tgaatttcca aatttcagtg
     6301 atctcttttg gatcagccat aaaattagac caaatatcgt agtggacagg aatcactact
     6361 tttgcattca atgactccgc catgcgcagc atatcaacag aagtaacttt gtctgttatc
     6421 cctcgtgggt tttcaccata ggcccctaaa cagacatcga ttttgtgttc attaccatgt
     6481 ttggcaaata aattggagta atgtgaatct cctgcatgat aaagattccc tcctgatgtt
     6541 tcgaataaat agtttactgc aatttgatcc atctcctgag gcatttttcc ttttaatacc
     6601 tcttctggat cttcacaagt gaccaacgct gtccgatcaa acgcttctaa agctactaca
     6661 ttgatgtctt tgattttgac tgcatcacct ggctttactg taatcgtttt ttcttcagga
     6721 attccccatt tttgccacgt gcgaaccact tcttttggcc caatgaatgt tgcttcagga
     6781 caattttgat gaactgctgc ggctgtatgg atatctagat gatcggaatg gatatgtgta
     6841 acgattaaag cgtctacatt ttttatcgca aaaggatcga ttacgaaagg ctgagtacgt
     6901 aaattaggct gcatattttg aacgccactc atacgcatca tctgatggcc ttttttcatc
     6961 atgccattcc catgactacg ttttcccgtc ccacaccata gatcacataa aatattcgtt
     7021 ccttcatgac ttttcagcca aattcccgta cagcccagcc accacatagc aaaagttcca
     7081 ggatccacta cttctgtttc gatttcttca ttcaagtatg tgccccactc aggaaatgtt
     7141 tctactaacc acttttcttt tgttacttca ttgacagttt tactcatttg cttgctcccc
     7201 ccatatcgtt gttccaactt acaagtaaat attaatgtgt atcagcatcc atgaaaaccc
     7261 ttttatatga ccgtttgttt tttatatgat tttctttcag taatcaatga ttgtttcagt
     7321 ataataaaga cagatatgaa tgtttaaatg aacaactagt caaataaatc catggatcaa
     7381 gatgtccctc aggaggcaaa agtcatgaaa aactcacttg tcaatattga aaaacgtcaa
     7441 aaagaaattt tggaactgct tcagcagact gaacaactat caactttcga attagctgaa
     7501 tcccttgatg tatctattag tacgatacga agagatttaa atttattaga agaaaaaaac
     7561 gagatcatac gcaattacgg ttattgttct tataattaca aaaaccagac tgattttgat
     7621 caatctggtc ctgaaaggat taaacaagcg attgccagag aagctagtaa atacattaac
     7681 gattatgata cgctgtttat caattctagc tcgactgcgt tgaaaacact ggattttctt
     7741 caatctaacc atttgacagt cattacaaac aacgtgaaaa tcaattactc tccgcacaaa
     7801 gataattgta gttatatttt aactggtgga gagatgcgct ttccaaaaga agcactagtg
     7861 ggcgacatcg cattaaatac gatttcttca gtcaatgcag acatttgtat cattggctgt
     7921 agcggagtcg atgtagtaca tggggtcaca acaaagatat taaatgaatc aacgatcaac
     7981 gaaatgatgg tcaacaagac aatcaaatgt aagattttag tcgctgatca tcgaaaaatc
     8041 ggactgacat caaagttcaa gattgctgat atttcagtct ttgattattt gatcactgat
     8101 aaattttgtt ctgaaaaagt attagaggag atcagaaata cggggataaa aaccattcaa
     8161 gtagattcat tttaagaacc aggacgtaaa tatgtaaata ggattatcaa aaaaatgaat
     8221 aacaaattta tcgataaatg aaaaacacgc tacgctcaaa ttgataaagg aatcccaaga
     8281 tgtatccatc caatcgatac catcataatc tattgcttct tttataaccg ttatatgttg
     8341 cctaaatagg aatcaaacaa tatcgaaaaa aggaggtatg acaaaagtca cacctccttt
     8401 tttcgaataa acggtattca aatcagaaca ctgatttcac aaccacttta tttcaggtcg
     8461 ttttcaatca cctttaccgc gtaatcattt ccgttgaaac gcgctatttt ttttgcgtcc
     8521 atatatcttt ctaaataatc atcttctcct aattgcttat ggcaagaagc aatttcataa
     8581 agcaagtatt ccaatgcata agtcgattta tgtttcttat tatactcaat acccttctca
     8641 cataattgga ttgctttatc ataatcctcg attttacggt aataggtggc gatatcataa
     8701 tatacaatgc agaccatcag attatcaaaa tcagtctcac tcaatatttt aagtgtacgc
     8761 tccacataaa ctttcgcaaa atctaattct ccttttgaca agtagatcat tgccacgttt
     8821 gcagctgctt gtaagtgatg gatatctgtt tcggtcgttt gttccaacac ttgattaaaa
     8881 taaaataatg ctgcatctgg ttttttcgca taaagatgat caagcagtcc ttgataaaat
     8941 aaaatttgat ttaaaagatc agaattactt actttttcct gatcgatcgt ttgtaaaatt
     9001 ttataagatt cttggtattt tgatacagaa ttcaatgatt gaatctcatt tatttttttc
     9061 ctgattttgt cttcttctgt ttctactaat atatcggaga tctcgacatt cagctttttg
     9121 caaagcgcac ttaagatatt gatgcctggc gacatattct tgttttcgat attgctgaca
     9181 gttgcttgtt cacaaatgcc ttctgctaat tgtacttgtg ttaacccctt tttctttcgg
     9241 tattctttta gtaatgctcc atctacttta atcaaaacaa tttccttccc tttaaaaaat
     9301 tatttctcct ttatatcata cacaaaaaaa agttctaaac aaccaaaaat gtttagaaca
     9361 gaataaaaat tttaatttta aaataatatt caaattaaac aactggcgta tctgctcctt
     9421 gatttctttt tgtgtta
//
LOCUS       DAZKLM010000099         9291 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3764
            SAMEA115706913-rid22582563.denovo.103, whole genome shotgun
            sequence.
ACCESSION   DAZKLM010000099 DAZKLM010000000
VERSION     DAZKLM010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706913
            Sequence Read Archive: ERR13196647
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9291)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9291)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287823.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287823.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,004
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,849
            CDSs (with protein)               :: 2,849
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 41 of 96
            Pseudo Genes (incomplete)         :: 52 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 15 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9291
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706913-rid22582563.denovo.103"
                     /isolate="VRE3764"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(22..690)
                     /locus_tag="ACG842_002338"
     CDS             complement(22..690)
                     /locus_tag="ACG842_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFQ0248848.1"
                     /translation="MKKLNLFTELCGIILGAGIYGLAVTGINIPSKLADGGVTGIALL
                     LHNLFGFAPSVTSLLFNLPLLLISLFLFGKHAFLRTIVGTFALVFFLHLWESLHIHFA
                     VDSLLINSLMTGILSGIGCGLVFRFGGSTGGTDIIYQAIEKYFHIKIGKSLFLITFGI
                     LVVSLLYLDLTHFLYTLLSCSILSYTLNKVKYLEIGSVLPQLEAKQTTETFEPLDPLE
                     QLEH"
     gene            824..1111
                     /locus_tag="ACG842_002339"
     CDS             824..1111
                     /locus_tag="ACG842_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0248849.1"
                     /translation="MRAADLLTILTKSPKKTEYQGVFLAQEQKLVPVTMVQINKANQF
                     IFLFEHGKAPLAMKDIFLFLMKNREKAVYYQSKEELIPLYGVKEIENKVVI"
     gene            1527..3065
                     /locus_tag="ACG842_002340"
     CDS             1527..3065
                     /locus_tag="ACG842_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HFQ0248850.1"
                     /translation="MNNFIQTLSERKGELLEAAFQHLSISLISLLIAALIAIPLAIWA
                     ANHKKMAEVLLQITGVLQTIPSLALLGLLIPFVGIGTVPALIALVIYALLPIFQNTYI
                     GLAEIDPSIEEAAVAFGMSRMRRLLKVELPIALPVIISGIRTALVLIIGTATLAALIG
                     AGGLGTFILLGIDRNTPVLTLIGAISSALLAIIFSGLIRWLQHKKPRYSIITLAAVVC
                     LFGGVSLYQSALFKNETITIAGKLGAEPEILIQMYKELIEDNTKTEVELKPNFGKTSF
                     LFNALDQGQIDIYPEFTGTVLESLVKVPEAMKSADLTKEKTYDEAEELLNKQFDMTLL
                     KPMAYQNTYALAVKRSFANEHQLKKISDLAAISDQIKAGFTLEFIDRSDGYKGIQEAY
                     GLNFSSVQSMEPSLRYQAINNGDVNIVDAYSTDSQLKEYDLVTLEDDRGLFPAYQGAP
                     LMKKDFAKEHPEIVKALNKLSGKITEDQMIDMNYQVNVEKKKPTEVARIFLKNEGLVK
                     EEDQ"
     gene            3062..4027
                     /locus_tag="ACG842_002341"
     CDS             3062..4027
                     /locus_tag="ACG842_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0248851.1"
                     /translation="MTKLIEFKHVQKKYDGKYVIDDLNLAIKKGEIFVLVGPSGSGKT
                     TTLKMINGLSKPSAGDIYFKGKSLNEYNLQKMRWNMGYVLQQIALFPTMTVKQNIEVI
                     PEMLGWEKQKRADRVDELLQKVGLSPDIYRDRMPRELSGGEQQRIGIIRAIAASPDVI
                     LMDEPFSALDPISRNSLQELVLSLHEELGTTIVFVTHNMEEAIKLGDRIAFMKDGEII
                     QCDTPEQLLMNPKNDYVRHFFDEPKQTKEWRVEDLVVNGYFLNEIPENARQQVCFDTP
                     MKEVYSLLSVYPSIVIVEKQRSIGSLTSKEIFAFLSREEEGNENI"
     gene            4014..5336
                     /locus_tag="ACG842_002342"
     CDS             4014..5336
                     /locus_tag="ACG842_002342"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFQ0248852.1"
                     /translation="MKTYDAIIIGSGVSGLSAAYGLKEAGKTVLVVEEDLWGGTCPNR
                     GCDPKKVLLSAVEARNRVKQLSGKGFNEIPTANWEELQKFKRTFTDPVPESRKKQLAE
                     AEIDHLSGTARFLDDSSIEVNEEVFHADYLVLATGQRPTILPVEGKEYLKTSADFLSL
                     PVLPKEIIFIGGGYIAFELATIANAAGSKVTIVHHNQRPLKEFEASLVEEAVHQMEAS
                     GIQFAFGVETQKIISEGTRYRLVGKETELVADMIFCATGRQPNTESLALEQANIVFDK
                     HGIAVNDYLQTSNPKIFACGDIVSRKTPKLTPVATFEGNYVAKRITDATSEPIKYPII
                     PTIVYASPKLAEVGVTKSHASSSDQVVEMDLTSWFTYHRVNEPVAKAELTFDQQNYLI
                     GAAVISEQADELIDDLTLVINQKLTKKELDSYIMGYPTLASDLSYLLK"
     regulatory      5399..5503
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            5577..6320
                     /locus_tag="ACG842_002343"
     CDS             5577..6320
                     /locus_tag="ACG842_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0248853.1"
                     /translation="MKKNLYPAVSMAFILIIWQIVVIVWKIPAFILPGPAAVFSALAA
                     DAQVLLSHSVTTLNEAVLGLVIASVLSFITALAMDYWKFLEMSFYPLLVISQMLPIMV
                     LGPLLTLWFGFGMLPKVILVVLISYFPIVVSFSDTLRKVSKEQIIFLKTMGADTGKIY
                     RIYKIPAGLSGFFSGLKVAATYCVSGAVVGEWLSAQAGLGYYMIRVKNSYQIDKVFAA
                     IICVILLSLLLNGACSLLKKGYYRMLYIF"
     gene            6335..7327
                     /locus_tag="ACG842_002344"
     CDS             6335..7327
                     /locus_tag="ACG842_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFQ0248854.1"
                     /translation="MKKLWLLLPLLLLLSACGTAKETSSKQEIKDLKKVTLVLDYVPN
                     TNHTGIYLAKEKGYYKEAGLNVQIIEPGDNSTSIGLVGADKAQFGVSYQEDVTYAHAD
                     GQNIPVKAIATVIKHNTSGFATLSDSNIHSPKDFEGKTYAGWQSPSEEAVLKAVMEKD
                     GGDFSKLTMVGSNGEGPESLGKSSDIQWYFEGWDMIKAKEAGIELNYIPLKELDERLD
                     YYTPVIITNDQLIKSDPELVQSFMDATKKGYQEAIKDPNDSAKLLQKYAKENDRTFLE
                     ESQAFLSKNYTDDPENWGLMEEKVWRNYTAFMQENGLIKQDVPSQELFTNQFIK"
     gene            7339..8076
                     /locus_tag="ACG842_002345"
     CDS             7339..8076
                     /locus_tag="ACG842_002345"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0248855.1"
                     /translation="MSIQISHLNVVIENLKILEDIHFTIPKDTFTSIVAPSGAGKSTL
                     LKTLTGVQPMTSGTIKVDDQKVTGLHTAFSYMPQEDMLLPWLSVYQNVTLYQKINHLT
                     INEEQVRDYLDIFGLNGYEHFLPEQLSGGMKQRTALLRTIMNPSSYLLLDEPFGALDA
                     MTRGQMQDWMLKLPKKAKRTTLLVTHDIEEAIYLSDRILVLSARPAHVIAEIQVPEKR
                     RSREWLLQQSELKQTIYQLLAGELDVK"
     gene            8066..8788
                     /locus_tag="ACG842_002346"
     CDS             8066..8788
                     /locus_tag="ACG842_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HFQ0248856.1"
                     /translation="MLSDAHCHLDGSQSLALLQQEHTILTIINCDSPEEWKENRQLAA
                     SKTQALSYGIHPWKADSYTFEQVEPFLKKARIIGEIGLDNIWTNVPMTTQKKVFERQL
                     AFAAINEKPVVLHTKGCEKEILNYIQAYPNHYLIHWYSSLDYQQDYIDLGCYFSIGLD
                     LKKNPAVWQLAKAVPIDHLLIETDGLHAAEWALGGIFSEKDYVSLLEEQLKTIAEIKE
                     LSVEEVKKNTYQNLERFIRLGK"
     gene            complement(8872..>9291)
                     /locus_tag="ACG842_002347"
     CDS             complement(8872..>9291)
                     /locus_tag="ACG842_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HFQ0248857.1"
                     /translation="YSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEQPVVQETVQTEK
                     QAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTATNGRYIGRYQLDSSYL
                     NGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
BASE COUNT         3042 a         1604 c         1965 g         2680 t
ORIGIN      
        1 taaagtagtg aaaaataaga atcagtgctc taactgttca agaggatcaa gtggttcaaa
       61 tgtttctgta gtctgttttg cttccaattg gggcaaaaca gatccaattt ccagatactt
      121 cactttattc aatgtataag atagaataga acagctaagt aatgtataca gaaaatgtgt
      181 gagatccaaa taaagcaagg agacaactaa aataccaaaa gtaatcaaga aaagactttt
      241 gccaatctta atatgaaaat atttttcaat ggcttgataa atgatatctg ttccgcctgt
      301 gcttccgccg aatcgaaaga cgagaccgca accaatgcca gaaagaatac ctgtcatcaa
      361 actattgatc agtagactat ctacagcaaa atgtatatga aggctctccc ataaatgtaa
      421 aaagaaaact agcgcaaatg ttccgacgat cgtccggaga aaagcgtgtt ttccaaaaag
      481 aaacaatgag ataagcaata aaggtaaatt aaaaagcagt gaagtgacag aaggagcgaa
      541 tccaaaaaga ttatgcaaaa gcaaagcaat accagtaacc ccaccatctg ccagcttaga
      601 tggaatattg attcctgtca cagctaatcc gtagattcct gcgcctaaaa tgattccgca
      661 caattctgta aataaattta attttttcat ttctagcctc ttttcttttg aaaattttca
      721 gcaacttctt tattatactg acttatactc ttacttcaag aggaaaatga aaaaaaatta
      781 tttttggctg tttcttttta tttatcaaat aaggagtgga aaaatgcgag cagcagactt
      841 attgacaatt cttacaaaat cacccaaaaa aacagagtat caaggagttt ttttggctca
      901 agaacaaaaa ctggtgcctg tgacaatggt ccagataaat aaagcaaatc aatttatctt
      961 tttatttgaa cacggcaaag caccacttgc tatgaaagac atctttcttt ttttgatgaa
     1021 gaaccgtgag aaagctgttt attatcagtc aaaagaagaa ctcattcctc tttatggagt
     1081 gaaagagata gaaaataaag tggtgatata agtaaaaata agaaaacaag acgtttatga
     1141 tgtcaaaaca accatttttt acatttttgg caaaaaagtg tcatgataca gtataatatt
     1201 cgttgaacag gccaataaag gtgttctccc ccacgagatt tctactttat tggctgtttc
     1261 tttttattaa aataaaagtt gtgtgtgtaa aaaagatata gctgattcca agtgacaatt
     1321 tcgtatagat ttttatggta ttcgtagtga aaaaacgaga accaaaaagt tgatccgact
     1381 ttgttgcttg gattcttttt ttgtcagcat atttagaggt atttttttct tttttttgtt
     1441 cgtgctgtaa ggttaaggca agacaagtta gttgggaaaa aggaaagaaa tgtttatact
     1501 taaagaataa cagaaaggaa ggtcgcatga ataattttat acaaacatta agtgaaagaa
     1561 aaggcgagtt gctggaagct gcatttcaac atctgtccat ttctctcatt tctttgctga
     1621 ttgcagcact gattgctatt cctttagcga tttgggcagc caatcataaa aaaatggcag
     1681 aagtcttatt gcagatcacg ggtgtgctcc aaacgattcc ttcacttgcc ttattaggtt
     1741 tacttatccc atttgtaggg atcggaactg ttccagcatt gattgcactt gttatttacg
     1801 ctttgcttcc gattttccaa aacacgtata ttggtctagc tgaaattgac ccgtctattg
     1861 aagaagcagc ggtagcattt ggtatgtcca gaatgagacg cttgctgaaa gtagagcttc
     1921 ccatcgcatt acctgtcatt atctcaggta tccgaactgc cttagtttta atcatcggta
     1981 cggctacttt ggcagcattg atcggagcag gtggattagg gacatttatt ttgttaggta
     2041 ttgaccgaaa tacacctgtt ttgacattga ttggtgcgat cagttctgcc ttgcttgcaa
     2101 ttatattcag cggactgatt cgctggttgc agcataagaa acctcgatat tccatcatta
     2161 ctttagcagc agtggtgtgt ttatttggcg gcgtttcttt atatcagtct gccttgttta
     2221 aaaatgaaac catcacgatt gctggaaaat taggagcgga acctgaaatc ctgatccaaa
     2281 tgtataaaga attgattgaa gacaacacaa aaacagaagt tgaattaaaa ccgaattttg
     2341 gcaagacgag ttttttgttc aatgcactgg atcaaggtca aatcgatatc tatcctgagt
     2401 tcacaggtac tgtcctagaa agcttggtga aggtacctga agcaatgaaa agtgctgatt
     2461 tgacaaaaga aaaaacatat gacgaagcag aagaattatt gaataagcaa ttcgatatga
     2521 cattattaaa accaatggct taccaaaata cgtatgcctt ggcagtcaag agatcttttg
     2581 cgaacgaaca tcaacttaaa aagatttccg atttggcagc tatctctgat cagatcaaag
     2641 cgggattcac actggaattt attgatcgtt cagatggata taaaggaatc caagaagcat
     2701 atggcttgaa tttttcaagt gtacaaagca tggagccaag tttgcgttac caagcaatca
     2761 ataatggaga tgtcaatatt gtggatgcct attccacaga tagccagctc aaggagtacg
     2821 atctagtgac tctagaagat gatcgagggt tattcccagc ttatcaaggc gcaccgctga
     2881 tgaaaaaaga ttttgctaaa gaacatcctg aaatcgttaa agcactgaat aaattaagcg
     2941 gaaaaataac agaggatcaa atgattgata tgaattacca agtcaatgtc gaaaagaaaa
     3001 agccgacaga agtagcacga atcttcctga aaaatgaagg attagtaaag gaggaagatc
     3061 agtgactaaa ttaatcgagt tcaaacatgt gcaaaaaaag tatgatggaa agtatgtcat
     3121 cgatgaccta aatctggcaa tcaaaaaagg agaaatcttt gttttggttg gtccttccgg
     3181 tagtggaaaa acaactactt tgaaaatgat caatggatta tctaaaccat cagcaggaga
     3241 catctacttc aaaggaaaat cgttaaatga atacaatctg cagaaaatgc ggtggaatat
     3301 gggatatgtg ctgcagcaaa tcgctttgtt cccgaccatg acagtcaagc aaaatattga
     3361 agtcatccct gaaatgttag gttgggaaaa gcagaaaaga gcagaccgtg tagatgaact
     3421 tcttcaaaaa gttggcttat ctcctgatat ttatcgagat cgtatgccta gagagttgtc
     3481 tggaggagaa cagcaacgga tcgggatcat ccgagctatt gctgcaagcc cagatgtgat
     3541 tttgatggac gagccattta gcgcattaga tccgatttca agaaactcat tacaagaatt
     3601 ggttttgtcc ttacacgaag aattgggcac gaccatcgtt ttcgttacgc acaatatgga
     3661 agaagcaatc aagctaggtg atcgtattgc atttatgaag gatggagaaa ttatccaatg
     3721 tgacacacca gagcagttat tgatgaatcc taaaaacgac tacgttcggc atttctttga
     3781 tgaaccgaaa cagacgaaag agtggcgagt agaagattta gtcgtcaatg gatatttctt
     3841 aaatgaaata ccagaaaatg ctcgtcaaca agtttgcttt gatacgccta tgaaagaagt
     3901 atattcgctt ctttcggtgt atccaagcat tgtgatagta gaaaagcaaa gaagtattgg
     3961 gtcgctgaca agtaaagaaa tctttgcttt tttaagtcga gaggaggaag gaaatgaaaa
     4021 catatgatgc aatcatcatc ggttcaggtg tgagcggctt aagtgctgct tatggattga
     4081 aagaagcagg taaaacagta ctagtggtcg aagaagatct atggggcggg acttgtccta
     4141 accgtggatg tgatcctaaa aaagtcttgt taagtgcagt agaggcacgt aatcgggtaa
     4201 aacaattgag tggaaaaggt tttaacgaga tcccaactgc caactgggaa gaacttcaaa
     4261 aattcaaacg aacatttacg gatccagtcc cagaaagccg aaaaaaacaa ttagcagaag
     4321 ctgaaatcga tcatctttca ggaaccgctc gttttttgga tgattcatct attgaagtca
     4381 acgaagaagt cttccatgcg gattacttag ttttggcaac cgggcaaaga cctacgatct
     4441 taccagtgga gggaaaggaa tacttgaaaa ctagtgcaga ctttttatca ttgcctgtac
     4501 tgcctaaaga aatcattttt atcggtggtg gttatattgc ttttgagcta gctacgattg
     4561 ccaatgctgc agggagcaaa gtaaccattg tccatcataa tcaacggcca ttaaaagaat
     4621 ttgaggcgtc tttggtagag gaagccgtcc atcaaatgga agcatctggc attcagtttg
     4681 cttttggtgt tgaaacccaa aagatcattt cagaaggtac tcgctatcgt ttagtcggaa
     4741 aagagacgga attagtagca gatatgattt tttgtgcgac tgggagacag ccgaatacag
     4801 aatctttagc acttgagcaa gcgaatatcg tctttgataa acacggaatt gcagtcaatg
     4861 attatctgca aacgagcaat ccaaaaattt ttgcgtgtgg agatattgtt tcaaggaaaa
     4921 ccccaaaatt gacaccagtc gccacttttg aaggaaatta tgtagctaag cggataacgg
     4981 atgcaacaag cgaacctatc aaatatccga ttatacctac gatcgtctac gccagtccta
     5041 aacttgctga agtaggagtt acaaaaagtc acgcctcttc gtcagatcaa gtggtagaga
     5101 tggatcttac cagttggttc acctatcatc gtgtcaatga accagttgct aaggctgaat
     5161 tgacctttga ccagcaaaat tatttgattg gtgctgcagt gattagcgag caagcagacg
     5221 aattgatcga tgatttgaca ctcgtcatca atcaaaaatt gactaaaaaa gagttggata
     5281 gctatatcat ggggtatccg acattggcaa gtgatctctc ctatttattg aaataatcag
     5341 catgataact aaagcgcttg ttgctttttc cttcttgtgc tacactttaa aaaatgaata
     5401 cctgtttctg gggatgtcgt agacgacatg agagtaagtt gtgaacttag acccattgaa
     5461 cctgtcagtt aatactggtg aagggagaaa catagcagac gcttgaagcg ggttactatg
     5521 tcgtgcccat ttcaagcgtt ttttgttatt catattagaa gaaaggaggt aacaaaatga
     5581 agaaaaatct ttatccagca gtcagtatgg cattcatttt aattatttgg cagattgttg
     5641 tcatagtttg gaaaatacct gcctttattt tacccggacc tgctgctgta ttttctgcat
     5701 tagcggcaga tgcacaagta ttactttccc attctgtcac gacattaaat gaagcagtgc
     5761 ttggtttggt aatagctagc gttctttctt ttattactgc cttggcaatg gattattgga
     5821 agtttttaga aatgtctttt tatccgttac tagtgatttc acaaatgttg ccaatcatgg
     5881 tgttagggcc attacttacc ctatggtttg gtttcggtat gcttccaaaa gtgatccttg
     5941 ttgtcttgat aagctatttt ccgatcgtag tttctttttc ggatacattg aggaaagtat
     6001 caaaagagca aatcatcttt ttgaaaacga tgggggcaga tacagggaag atttatcgaa
     6061 tatataaaat accagcgggt ttatccggct tcttttccgg tttaaaagtg gctgctactt
     6121 attgtgtcag cggagcagtc gttggagaat ggctaagcgc tcaagcaggc ttagggtatt
     6181 acatgatacg cgtaaaaaac agctaccaga tcgataaagt ttttgctgcg atcatttgtg
     6241 tcattctatt gagtttattg ctgaatggcg cttgttcatt attgaaaaaa gggtattatc
     6301 gtatgcttta tattttttaa gagaagggga tcaaatgaaa aaattatggc ttttattacc
     6361 gctactgttg cttttatccg cttgtggcac tgcaaaagaa acaagtagca aacaagaaat
     6421 aaaggatttg aaaaaagtga cacttgtttt ggattatgta ccgaatacta atcatactgg
     6481 catctaccta gcaaaagaaa aaggctatta taaagaggct ggtttaaatg tccaaatcat
     6541 cgaaccagga gataattcga caagtattgg tctagtaggt gcggacaaag cacaatttgg
     6601 tgtaagttat caagaagatg tcacttatgc tcacgcagat ggacagaata taccagtcaa
     6661 agcaattgct acagtgatca aacataatac ttctggattc gccaccctat cagacagtaa
     6721 catccattca ccaaaagatt ttgaaggaaa aacatatgca ggttggcaaa gtccaagtga
     6781 agaagctgtc ttaaaagctg tgatggaaaa agacggcggg gacttttcaa aactgacaat
     6841 ggtaggaagc aatggagaag ggccagaaag cttaggaaaa tcctcagata tccaatggta
     6901 ctttgaaggt tgggatatga tcaaagcaaa agaagcgggt attgaactaa actatatccc
     6961 attgaaagaa ttagatgaac ggttagatta ctatactccg gtaattatta cgaatgatca
     7021 actgatcaaa agtgatccag aattggtaca gtcttttatg gatgccacga aaaaaggcta
     7081 tcaggaagct attaaggatc caaatgacag tgctaaactg ttgcaaaaat atgcaaaaga
     7141 aaatgatcgg acgtttttag aagaatcaca agcattcttg tcaaaaaatt atacggatga
     7201 tcctgagaat tggggattga tggaagaaaa agtttggcgc aattatacag cttttatgca
     7261 ggaaaacgga ttgatcaagc aagatgtacc ttcgcaagaa ctattcacta atcagtttat
     7321 aaaataggag tgatagatat gtcaatccaa atcagccact tgaatgtagt cattgaaaac
     7381 ctgaaaatat tagaagacat ccatttcact atccctaaag atacctttac atcgattgtc
     7441 gcgccaagcg gagccggtaa gtcaacattg ctgaaaactt taacaggagt ccagcctatg
     7501 acttctggaa cgatcaaagt agacgatcaa aaagtaactg gtctccatac agcatttagt
     7561 tatatgccgc aagaagatat gttacttcct tggctgagcg tctatcaaaa cgttacgtta
     7621 tatcaaaaaa tcaatcatct aacaatcaat gaagaacaag taagggatta tctggatatc
     7681 tttgggttaa acggctatga acattttttg ccggaacaat tatctggcgg gatgaagcag
     7741 cgaacggcct tgttacgaac aatcatgaat ccttcttctt acttgcttct tgatgaacca
     7801 tttggagcgt tagatgctat gaccagaggg cagatgcaag actggatgtt gaagctgcct
     7861 aaaaaagcaa agcggactac tttacttgtg acgcatgata tcgaagaagc aatttattta
     7921 tcagacagga ttcttgtgct ttccgcccgt ccggcacatg tgattgctga gattcaagtg
     7981 cctgaaaaac gacggtcacg tgaatggcta ctgcagcaat cagagttgaa gcaaacgatt
     8041 tatcagttat tggcaggtga attagatgtt aagtgatgct cattgtcatt tagatgggag
     8101 tcagagttta gcactccttc agcaggaaca cacgattttg acgatcatca attgtgattc
     8161 gccagaagaa tggaaggaaa acagacagct tgcagcaagt aagactcaag ccttaagtta
     8221 cggtatccat ccttggaaag cagatagcta tacgtttgaa caagttgagc cttttttgaa
     8281 aaaagcaagg atcatcggtg aaatagggtt agataatatt tggacgaatg taccgatgac
     8341 tacacaaaaa aaagtatttg agcgacagct tgcttttgca gccataaatg agaaacctgt
     8401 tgtactgcac acaaaaggat gcgaaaaaga gatcttaaat tatatccaag cttaccctaa
     8461 tcactatttg atccattggt attcttcttt ggactatcaa caagactata ttgatttagg
     8521 ctgttatttc agcattggtc ttgatttgaa aaagaatcca gcagtctggc aactagctaa
     8581 agcagtccca atcgaccact tacttattga aacggatgga ctacatgcag cagagtgggc
     8641 tttagggggg attttttcag aaaaagatta tgtctcactg cttgaagaac agttaaagac
     8701 aatcgctgag atcaaagagc tatcagttga agaagttaaa aaaaatactt atcaaaactt
     8761 ggaacgtttc attagattag gtaaataaaa aagagatgtg acacaactag tgtcgcacct
     8821 ctttttggct ttctttgtat aaaagtaaac tgtttgatta ataaatgaat cttagtacca
     8881 accgtttgct tcccagaatg ctttcgccgc ttcccaagaa ccataacgtg aagtaacata
     8941 ttgttctgct actctttctt ggtttgcagc agagtagtca ccatttaagt aagaagaatc
     9001 taattggtaa cgtccgatgt aacgtccgtt cgttgctgtg taagaaccgc ttgattcttt
     9061 ttgtgcgatc cattcttttg cagagcttgt gcttgctgga gtagcagcag tttctgttac
     9121 agcaggagca ggttgtgttt cagcaacagg cgctgcttgt ttttccgttt gaaccgtttc
     9181 ttgtacaaca ggttgttcag cttggactgg tgcttcttga acggctgctt gaggttcagc
     9241 agctgcttgt gcagagcctt ctgttgggat tgttaattgt tgtcctgagt a
//
LOCUS       DAZKLM010000100         9089 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3764
            SAMEA115706913-rid22582563.denovo.104, whole genome shotgun
            sequence.
ACCESSION   DAZKLM010000100 DAZKLM010000000
VERSION     DAZKLM010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706913
            Sequence Read Archive: ERR13196647
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9089)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9089)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287823.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287823.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,004
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,849
            CDSs (with protein)               :: 2,849
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 41 of 96
            Pseudo Genes (incomplete)         :: 52 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 15 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9089
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706913-rid22582563.denovo.104"
                     /isolate="VRE3764"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(46..366)
                     /locus_tag="ACG842_002348"
     CDS             complement(46..366)
                     /locus_tag="ACG842_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HFQ0248858.1"
                     /translation="MKLVNVTNSHSRLVLNQLENTDAHLVKVYTAGNTTIVYTEAPQH
                     NEILLINDKRKIQPKEIEDAKNYFLDKIKDAVYHEDEIKVIELAGLVEISIPKHELGA
                     TLAV"
     gene            complement(514..2748)
                     /locus_tag="ACG842_002349"
     CDS             complement(514..2748)
                     /locus_tag="ACG842_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFQ0248859.1"
                     /translation="MLCQNCGKNEATIHLYATVNGQRTQLDYCQSCYQKIKNQQNGGL
                     TNMAQNDPFGFGSLDDLFRSMSRQMQQQGNYEQTPPTQFGGNNNFNGGQPPQGNTEGL
                     LGEYGINITEQARQGDIDPVIGRDDEIKRVIEILNRRTKNNPVLIGEPGVGKTAVVEG
                     LAQKIVDGDVPQKLMDKEVIRLDVVSLVQGTGIRGQFEERMQKLIEEIRQAENVILFI
                     DEVHEIVGAGSAGDGNMDAGNILKPALARGELQMVGATTLNEYRIIEKDAALERRMQP
                     VRVDEPTVEETISILKGLQKRYEDYHHVKYTDEAIEAAATLSNRYVQDRFLPDKAIDL
                     LDETGSKKNLTIQIVDPKTIEKKLQEAEEQKVLASREEDFEKAAYYRDQINKLQKMKE
                     RQLTEEETPVITEKDMEKIVEQRTGIPVGELKEKEQTQLKNLADDLKAHVIGQDNAVD
                     RVAKAIRRNRVGLNKQNRPIGSFLFVGPTGVGKTELAKQLAYELFGSQDSMIRFDMSE
                     YMEKHSVSKLIGSPPGYVGYEEAGQLTEKVRRNPYSLVLLDEVEKAHPDVLHMFLQIL
                     DDGRLTDAQGRTVSFKDTIIIMTSNAGTGKVEANVGFGAAREGVTRSVLNQLNNYFTP
                     EFLNRFDGIIEFSALSKENLMTIVTLMLDDVNQMLAAQQLHIEVPTNVKEKLVDLGYD
                     PSMGARPLRRTIQEQIEDGIAEFYLDHPSIHELKAKLDKDGKIIVTSKPERLAKESAE
                     ETAE"
     gene            3227..3493
                     /locus_tag="ACG842_002350"
     CDS             3227..3493
                     /locus_tag="ACG842_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010773230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HFQ0248860.1"
                     /translation="MEKKEFHVVAETGIHARPATLLVQTASKFNSDVNLEYKGKSVNL
                     KSIMGVMSLGVGQGSDVTITAEGADEADAMAAIVETMKKEGLSE"
     gene            3493..5220
                     /gene="ptsP"
                     /locus_tag="ACG842_002351"
     CDS             3493..5220
                     /gene="ptsP"
                     /locus_tag="ACG842_002351"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HFQ0248861.1"
                     /translation="MVEMLKGIAASDGVAVAKAYLLVQPDLTFSKATVEDTAAEEARL
                     DAALAKSTEELQQIREKAAQSLGEAEAQVFDAHLMVLSDPEMVGQIKQNIKDNSVNAE
                     SALKEVTDMYIGMFEAMEDNAYMQERAADIRDVAKRILAHLLGVTLPNPSMINEEVVV
                     VAHDLTPSDTAQLDRNFVKAFVTDIGGRTSHSAIMARSLEIPAIVGTKEITAKVKEGD
                     ILAVNGIEGDVIIDPTDEQKAEFEKAGADYAAQKAEWEKLKNAETVTADGKHFELAAN
                     IGTPKDLVGVHNNGGEAVGLYRTEFLYMDSPDFPTEDDQYEAYKAVLEGMEGKPVVVR
                     TMDIGGDKELPYLQLPHEMNPFLGYRALRISLSEQGDEMFRTQMRALLRASVHGNLRI
                     MFPMVATLKEFRAAKAIFEEEKQKLISEGKEVSDTIQVGIMIEIPAAAVLADKFAKEV
                     DFFSVGTNDLIQYTMAADRMNERVSYLYQPYNPSILRLIKNVIDAAHAEGKWAGMCGE
                     MAGDQTAVPLLVGMGLDEFSMSATSILKTRSLMKRLDTAKMAELADRALKECDTMEEV
                     VELVHEYVK"
     gene            5384..6430
                     /locus_tag="ACG842_002352"
     CDS             5384..6430
                     /locus_tag="ACG842_002352"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFQ0248862.1"
                     /translation="MKVLLYFESEKMLAKSGIGRALDHQKRALTEVGISYTLDEKEDY
                     DILHINTYGINSHNMVNKARREGKKVVYHAHSTEEDFRNSFIGSNQLSPIVKKYLVGL
                     YQKADYLITPTPYSKQLLESYGIRVPIQAISNGIDLEKYHPDPMKEQKFREYFKLSPD
                     QKVIICVGLFFERKGIIDFVEIAKKMPEYTFIWFGHVPMYSIPRNIRKIVKEDHPENV
                     LFPGYIRGEIIEGAYSGADLFFFPSYEETEGIVVLEALASKQNVLVRDIPVYNGWLED
                     SKNCYMGKTNEEFIRLIQQITNQELPSTTQAGYQTAKERSINKIGEELKNVYESVLNE
                     KVSSKLKKVNQVKD"
     gene            6447..7670
                     /locus_tag="ACG842_002353"
     CDS             6447..7670
                     /locus_tag="ACG842_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010706866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFQ0248863.1"
                     /translation="MKIGFFTDTYFPQVSGVATSIKTLKQELERHGHEVYIFTTTDPN
                     ADKLEKDVIRMPSVPFISFKDRRVVVRGMWYAYLVAKELELDLIHTHTEFGAGILGKM
                     VAKKLKIPLIHTYHTMYEDYLHYIGKGKVIRQSHVKYLSRLFANHTTGVVCPSERVID
                     TLRSYGVIAPLRVIPTGIDIEKFKRSDITQQDISKLRESLGLQENHLMILSLSRISYE
                     KNIQALINGFPQVISAYPNARLVIVGKGPYVEELEELISELQLEEFVQFTGEVDNNDV
                     ALYYKAADYFVSASTSETQGLTYTEAMAAGTPCVVEGNAYLNRLFDHPSLGKTFETDE
                     DFAPALIDYISSEVPKDPKVLEAKLYDISSTHFGEAMIEFYQDMVVYHEEEMRRKEEE
                     DSIERIKIKLNSFKR"
     gene            7759..8646
                     /locus_tag="ACG842_002354"
     CDS             7759..8646
                     /locus_tag="ACG842_002354"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HFQ0248864.1"
                     /translation="MKLGFIGTGVMGSAVARHLLEAGHEVAVYNRTKAKADPLVTEGA
                     IWADTPKAVAEQSNILFTMVGYPKDVEEIYYGQSGIFSADISGHILVDLTTSTPSLAE
                     KIAKTAKEKGADALDAPVSGGDLGAKNGTLTIMVGGEEAVYDQVLPLFKEFGTTFTLH
                     GSAGKGQHTKMANQIMIAGTMTGMTEMLVYAQKNGLDLKKVIETLSGGSAANWSLSNY
                     SPRILKEDYTPGFFVKHFIKDLKIALEEAEKMDLVLPATTQALKLYEELADKGFENDG
                     TQALIKLWWPEGKIPEKKS"
     gene            8732..8962
                     /locus_tag="ACG842_002355"
     CDS             8732..8962
                     /locus_tag="ACG842_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkuJ family protein"
                     /protein_id="HFQ0248865.1"
                     /translation="MTHSQLVAIIKRLEAMVESADNELQVRRFEKEGVEKCTVTYDKA
                     TDTFELTETDTHQQYEFDNIDIVAMEIYDLIQ"
BASE COUNT         2909 a         1614 c         1858 g         2708 t
ORIGIN      
        1 tttacttttg tcccacgctc tttaattatt atgttcaaaa tttgtttaca cggctaatgt
       61 agcgcctaat tcatgttttg gaatcgagat ttcaacaagg cctgccaatt cgattacttt
      121 gatttcatcc tcgtggtaaa cggcgtcttt gattttatct aagaaataat ttttagcatc
      181 ttcgatttct ttcggctgga ttttacgttt gtcattgatc agcagaattt cattatgttg
      241 aggggcttca gtgtagacaa tagtggtatt tccggctgtg tagaccttga ctagatgggc
      301 atcagtgttt tcaagctggt ttaatacaag acgggaatgg ctgtttgtta catttaccaa
      361 tttcatggcg ctcacctctt ttatgtattt ttaattgctt gtttctctta ttataatttc
      421 ttttttataa aatgaacagt attttgactt ttctgaaaaa agaatgagac ggcatttctc
      481 aaagaaatac cgtctcacgc tataaaactg gatttattca gccgtttctt cggctgattc
      541 tttagccaaa cgttctggtt tagaagtaac gatgattttt ccatctttat ccagttttgc
      601 tttcaactca tggatagaag ggtgatcaag ataaaattca gcgataccat cttcgatttg
      661 ttcttggatc gtgcgtcgaa gaggacgagc acccatagat ggatcatagc ctaagtcaac
      721 taatttttct ttgacattcg taggaacttc gatgtgaagt tgctgtgctg ccagcatttg
      781 gttgacatcg tctagcatta gtgtgacgat tgtcatcaag ttttcttttg ataatgcact
      841 gaattcgatg atcccgtcaa aacggttcag aaattctggt gtaaagtagt tgtttaattg
      901 gttcaataca gaacgagtaa caccttcgcg tgccgcacca aaaccaacgt ttgcttctac
      961 tttaccagtt ccggcattac ttgtcataat gatgatcgta tctttaaagc tgaccgttct
     1021 tccttgtgca tctgtcaatc gaccatcatc taagatttgc aagaacatat gcaaaacatc
     1081 tggatgagct ttttctactt catctaacaa gacaagactg taaggatttc tccgtacttt
     1141 ttctgttaat tgaccagctt cttcgtatcc gacataacct ggaggagaac caatcaattt
     1201 agagacacta tgtttttcca tatattcaga catatcgaaa cgaatcattg aatcttgtga
     1261 accgaatagt tcgtaggcaa gttgtttcgc aagctctgtt ttacctacac cagttggtcc
     1321 aacaaataag aaggagccga ttggacgatt ctgtttgttt aatccaacac ggtttcggcg
     1381 aatcgctttc gctactcgat ctacagcgtt atcttgtccg atgacatgag ctttcaagtc
     1441 atctgctaag tttttcagtt gtgtctgttc tttttctttt aattcaccca ctggaatacc
     1501 tgtccgttgt tcaacgattt tttccatatc tttttcggta atcacaggtg tttcctcttc
     1561 agtgagctga cgttctttca ttttttgaag tttattgatc tgatcgcgat aataagctgc
     1621 tttttcgaaa tcttcttctc gagaagctaa aactttttgt tcttctgctt cttgcagttt
     1681 tttctcgatt gttttaggat caacgatttg gatcgtcaag tttttcttag aacctgtttc
     1741 atctagcaaa tcgatggctt tatccggcaa gaagcgatct tggacgtaac gattggataa
     1801 agttgcggct gcttcgatgg cctcatcagt atattttaca tgatgatagt cttcataacg
     1861 tttttgcaaa cctttcaaaa tactgatcgt ttcttcgaca gttggttcat ctacccgtac
     1921 aggttgcata cgacgttcta aagcagcgtc tttttcgatg atgcggtact catttaaggt
     1981 agtagcacca accatttgca attctcctcg agctaaagct ggttttaaaa tattaccagc
     2041 atccatattt ccatcaccag cagaaccagc accgactatt tcgtgaactt catctatgaa
     2101 taggatcaca ttttctgctt gacggatctc ttcaatcaat ttttgcatac gttcttcaaa
     2161 ttgtcctcgt ataccagtac cttggactaa agatacgaca tctaaacgaa tgacttcttt
     2221 gtccattaat ttttgcggta catcaccatc tacgattttt tgtgctaaac cttcaacaac
     2281 tgctgtttta ccaacacctg gttctccaat caagacagga ttgttcttgg ttcggcgatt
     2341 taaaatttca atgactcgtt tgatttcgtc atcgcgtccg atcacaggat cgatatcacc
     2401 ttgtcgtgct tgttctgtga tattgattcc gtattctccc aataaacctt ctgtattgcc
     2461 ttgaggtggc tgaccaccat tgaagttgtt gtttccgcca aattgtgttg gtggtgtttg
     2521 ctcataattt ccttgctgtt gcatttggcg agacatggaa cggaaaagat cgtccaaact
     2581 gccaaatcca aatggatcat tttgggccat attagtcaga cctccatttt gttgattttt
     2641 gattttttga taacagctct gacagtaatc gagttgtgtc cgctgaccat tgacagtggc
     2701 atacaagtga atcgttgcct cattcttgcc gcagttttga caaagcatga atcattcacg
     2761 tcctttcaca aaaatgggta catccatatt ttacatctcg tttttcatga ggtaaaagaa
     2821 taacccctag ttttttaaga gatttatgag gtagaaaagg tgaaatctct attgaccatt
     2881 cctgaccttt gactcttatt atactaaaaa taaaaaggga agcaagtaat tcgtttcata
     2941 tgaaaacaaa gagggtcaaa ggaaagaatg ataaacgtca ggagaagaga aagcgacaag
     3001 aaaaaggaaa taaacacgtt tgttataaaa tgtgaagaaa aggatgaaaa agaatccgga
     3061 ataatttgaa tacttttatc tgaaattgaa tgaaaactgt tgtatctaac gaaaaaaaat
     3121 gttaatcttt acagatgaaa gggtggcatt tgttaggatt ctctgaatta ctaaaaaaga
     3181 aaaccctcac ataaaaacaa cactcactta aaggagaccg attaatatgg aaaagaaaga
     3241 atttcacgta gtagcagaaa ctgggatcca cgctcgtcca gctacactat tagtacaaac
     3301 agcaagcaaa tttaactctg atgtaaactt agagtataaa ggtaaatcag taaacttgaa
     3361 atcaatcatg ggcgttatgt ctttaggtgt aggtcaaggt tctgatgtta ctattactgc
     3421 agaaggtgct gacgaagctg atgcaatggc agcaatcgtt gaaacaatga agaaagaagg
     3481 cttatctgaa taatggttga aatgctaaaa gggattgccg ctagtgacgg tgtagccgtt
     3541 gcaaaagctt acctgctagt tcaaccggat ttaacattca gtaaggcaac agtagaagat
     3601 actgcggctg aagaagctcg tttggatgct gcattagcta aatctactga agaattgcag
     3661 caaattcgtg aaaaagcagc gcaaagctta ggtgaagcag aagcgcaagt atttgatgcc
     3721 catttgatgg ttctttcaga tccagaaatg gtaggtcaaa tcaagcaaaa tattaaagac
     3781 aacagcgtaa atgctgaatc agcgctaaaa gaagtaacgg atatgtatat cggtatgttc
     3841 gaagcaatgg aagataatgc ttatatgcaa gagcgtgctg cagatattcg tgacgttgct
     3901 aaacgtattt tagcgcactt attaggcgtt actttaccaa atccatcaat gatcaacgaa
     3961 gaagtggtcg ttgttgcaca tgacttgact ccaagtgata cagcacaatt agatcgcaat
     4021 tttgtaaaag cgtttgtaac agacattggt ggacgtactt cacattctgc tatcatggca
     4081 cgttctttag aaattccagc aatcgttggg acaaaagaaa ttactgctaa agtcaaagaa
     4141 ggcgatattt tggctgtcaa cgggatcgaa ggcgatgtaa tcatcgatcc tacagatgaa
     4201 caaaaagctg aatttgaaaa agcaggtgca gattacgcag cacaaaaagc agaatgggaa
     4261 aaattaaaaa atgcagaaac tgtcacagct gacggcaagc attttgaatt agctgcaaac
     4321 atcggaacac caaaagatct agttggtgtc cataataatg gtggagaagc agttggtctg
     4381 taccgtactg aattcctgta tatggattct ccagacttcc caacagaaga tgaccaatat
     4441 gaagcttaca aagctgtatt agaaggtatg gaaggaaaac ctgtagttgt tcgtacaatg
     4501 gacatcggtg gagataaaga actaccatat ctgcaattac ctcatgaaat gaatccattc
     4561 ttaggatatc gtgcattgcg tatcagttta tctgaacaag gcgacgaaat gttccgtaca
     4621 caaatgcgtg cattgcttcg tgcatctgtt catggtaatt tacgtatcat gttccctatg
     4681 gtagctacac tcaaagaatt ccgcgcagca aaagcgatct ttgaagaaga aaaacaaaaa
     4741 ttgataagtg aaggcaagga agtatctgat acgatccaag tcggcatcat gatcgagatt
     4801 ccagcagcag cagtcttagc agacaagttt gcaaaagaag tagatttctt ctctgtaggt
     4861 acgaacgatt tgattcaata cacaatggca gcagaccgta tgaacgaacg cgtttcttac
     4921 ttgtaccaac catataaccc atcaatttta cgtttgatca aaaatgtaat tgatgcagca
     4981 cacgctgaag gaaaatgggc aggtatgtgt ggagaaatgg ctggagatca aacagctgtt
     5041 ccattgctag taggtatggg tctagatgag ttctcaatga gtgcgacatc tattcttaaa
     5101 acgcgtagtt taatgaaacg cttggacaca gcgaaaatgg ctgaacttgc tgatcgtgca
     5161 ttgaaagaat gcgatacgat ggaagaagtc gttgaacttg ttcatgaata tgtaaaataa
     5221 acaacgtatc aaaagctggt gtggcttgta aaagagctac gccagttttt ttgttctttt
     5281 ttaagaaaac gtttttgtca gtaagaatag acaaaaatag ggcttaagtt tgagtgacag
     5341 tttgtaggct atgttatact atataagtga agggggacgt aaggtgaagg tattattata
     5401 ttttgaaagt gaaaagatgc ttgcaaaatc tggaatcggg cgtgcgcttg atcatcaaaa
     5461 acgtgctctg acggaagtcg gtatctctta tacactagat gaaaaagaag attatgacat
     5521 cctgcacatc aatacatacg gaatcaatag tcataatatg gtcaataaag ccagaagaga
     5581 aggaaaaaaa gttgtctatc atgcgcattc tacagaggaa gatttccgaa attcctttat
     5641 cggatcgaac cagctttctc ctatcgtaaa aaaatatctg gtaggattgt accaaaaagc
     5701 ggactatctg atcacgccaa caccttattc aaaacagttg ttggaaagct atggtatccg
     5761 tgtgccgatt caagctattt ccaatgggat cgacttagag aaatatcacc ctgatccaat
     5821 gaaagaacaa aagttcagag aatatttcaa actgtctccg gatcaaaaag taatcatttg
     5881 tgtcggtttg tttttcgaac gtaaaggaat cattgatttc gtagaaattg ctaagaagat
     5941 gcccgaatac acatttattt ggtttggtca tgttcccatg tactcgattc ctcggaacat
     6001 ccggaaaatt gtaaaagaag atcatcctga aaatgtcctg tttcctggtt acattcgtgg
     6061 agagatcata gaaggtgcgt attcaggtgc agatctattc tttttccctt cttatgaaga
     6121 gacagaaggg atcgttgttt tagaagcttt agcaagcaaa caaaacgtac ttgttcgaga
     6181 tattccagtg tataatggct ggttagaaga tagcaagaac tgctatatgg ggaagacaaa
     6241 cgaagaattt atccgactga tccaacagat taccaatcaa gaactaccga gtacgacaca
     6301 agccggttat caaacagcaa aagagcgaag tatcaataaa atcggtgaag aacttaaaaa
     6361 tgtatacgaa agtgtactga atgaaaaagt ctctagcaaa ttaaaaaaag taaatcaggt
     6421 caaggactag tttggaggcg aaaaacatga aaatcggctt ttttaccgat acctactttc
     6481 cgcaagtcag cggagttgct acatcgatca aaacattaaa acaagaatta gaaagacatg
     6541 ggcatgaagt ctatattttt acaacgaccg atcctaatgc ggataagttg gaaaaagatg
     6601 tgattcgaat gccaagtgta ccatttattt ctttcaagga tcgaagagtt gttgttagag
     6661 gcatgtggta tgcttatttg gttgccaaag aactagaact agatcttatt catacccata
     6721 cagaattcgg agcagggata cttgggaaga tggtagctaa gaagttgaaa atacctctga
     6781 tccatactta ccatacgatg tatgaagact accttcatta tatcggtaaa ggaaaagtga
     6841 tcaggcaatc ccatgtaaaa tacttgtcta gattatttgc aaatcatacg acaggtgtag
     6901 tttgtccaag tgaacgtgtt attgatacat tacgcagcta tggtgtaata gcaccgcttc
     6961 gcgtgattcc tactggtatc gacattgaaa aattcaaacg ttctgatatc actcagcagg
     7021 atatcagcaa attaagggag tcactaggtc ttcaggaaaa ccatttgatg attctttctt
     7081 taagcaggat ttcttatgag aaaaatatcc aagccttgat taatgggttt cctcaggtca
     7141 tctccgctta tcctaacgcc agattagtta tcgtaggaaa aggaccttat gtagaagagt
     7201 tagaggaatt gatctcagaa ctgcagttag aagaatttgt gcagttcaca ggagaagtgg
     7261 acaacaatga tgtagcactt tattataaag cggcagatta ttttgtcagt gcttctactt
     7321 cagaaacaca agggcttact tatacagaag cgatggcagc cggaacacct tgtgtcgtgg
     7381 aaggaaatgc ttatttgaac cgattgttcg atcatccttc attagggaaa acttttgaaa
     7441 ccgacgaaga ctttgcacca gcacttattg attatatttc cagtgaagtt cctaaagatc
     7501 caaaagtttt agaagctaaa ctttatgata tttcatctac acatttcggc gaagctatga
     7561 tcgaatttta tcaagacatg gttgtttatc atgaagaaga gatgcgaaga aaagaagaag
     7621 aagactcgat tgaacgaatc aaaataaaat taaattcatt taaaagataa aattcaaaca
     7681 ttttttatga aaaaatcctt gtttctagcg tatactagca tagaagcaag gatttttgat
     7741 ttttaggagg tcgaaaaaat gaaacttggt tttatcggaa caggcgtcat gggaagtgct
     7801 gtcgcacgcc atctactaga agcaggacac gaagtagctg tctataatcg gacaaaagca
     7861 aaagctgatc ctttagtaac agaaggagct atctgggcag atacaccaaa agcggtagca
     7921 gaacaaagta atattctctt tacaatggta ggttacccca aagatgtaga agaaatctac
     7981 tatggacagt cggggatttt ttctgcagat atctcaggac atattttagt tgatttgact
     8041 acaagtacac caagcctggc agaaaaaatt gcaaaaacag caaaagaaaa aggcgcagat
     8101 gcgttagatg caccggtatc cggtggtgac ttaggagcaa agaatggtac actgacgatc
     8161 atggtcggtg gagaagaagc cgtttatgat caagtactgc ctttattcaa agaatttggt
     8221 acgacattta cattgcatgg ttctgctggg aaaggtcagc atacaaaaat ggctaatcag
     8281 atcatgattg caggtacgat gacaggaatg acggaaatgc ttgtctatgc acaaaaaaac
     8341 ggacttgatt taaagaaagt gattgagacg ctttcaggtg gaagtgcagc taactggtct
     8401 ttaagcaatt attctcctcg tattttaaaa gaagattaca cacctggttt ttttgtcaaa
     8461 cattttatca aagatttaaa gattgcatta gaagaagcag aaaaaatgga tcttgtattg
     8521 ccagcaacta cccaagcatt gaaactatat gaagagttgg cagataaagg ttttgagaat
     8581 gacggaaccc aagctttgat caaactttgg tggcctgaag gaaaaatacc agaaaaaaaa
     8641 tcataaaaaa cgagtttttt tagagaaaat caatgcaagt aggctatgct tttgatacaa
     8701 tataacagaa ataagaaaag gaggacctcc gatgacacat tcacaattag tcgcaatcat
     8761 aaaacgactg gaggctatgg tcgagtcagc agataatgaa ctgcaagttc gccgttttga
     8821 aaaagaaggc gtagagaagt gtacagtaac ttatgataaa gcgaccgata catttgaatt
     8881 aactgaaaca gatactcatc agcaatacga atttgataat atcgatatcg tagcgatgga
     8941 aatctatgac ttaatccaat aaattttagg ataattgaca tttgaagttg aagatgccta
     9001 aaagaagccg agaagcaact aatttagttg cttttcagct tcttttttgt ttaacagttc
     9061 aatttcaaac tgttcttttt ctaattttt
//
LOCUS       DAZKLM010000101         8942 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3764
            SAMEA115706913-rid22582563.denovo.105, whole genome shotgun
            sequence.
ACCESSION   DAZKLM010000101 DAZKLM010000000
VERSION     DAZKLM010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706913
            Sequence Read Archive: ERR13196647
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8942)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8942)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287823.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287823.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,004
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,849
            CDSs (with protein)               :: 2,849
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 41 of 96
            Pseudo Genes (incomplete)         :: 52 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 15 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8942
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706913-rid22582563.denovo.105"
                     /isolate="VRE3764"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..260
                     /locus_tag="ACG842_002356"
     CDS             <1..260
                     /locus_tag="ACG842_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0248866.1"
                     /translation="LKRKVTDRNKNLLNVVMICYISFLILGLPQAVFPEMFSGDWGIV
                     SYSILTLIAMVLGTSFYNAFVSKHVEEYEVLMYLKEKKTSN"
     gene            467..667
                     /locus_tag="ACG842_002357"
                     /pseudo
     CDS             467..667
                     /locus_tag="ACG842_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298761.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1379..1984)
                     /locus_tag="ACG842_002358"
     CDS             complement(1379..1984)
                     /locus_tag="ACG842_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFQ0248867.1"
                     /translation="MAKIGYIRVSSQDQNLDRQLAMMEDLNIDKLFQEKASGKDIQRP
                     EFQKLLNYIREGDCVIVASLDRLGRDYEDIKNTVTFMKQKKVALKILDAQFLDFNTGN
                     ELLDTAMFDMFLSLLSYIAQNEREKIRERQRQGVLLAKKAGRYKGRPIEYSENSTDPQ
                     KRLVFKTVVDMLNQGVPVAEIAKENGLSRPTIYKIKKANDL"
     gene            2166..2501
                     /locus_tag="ACG842_002359"
                     /pseudo
     CDS             2166..2501
                     /locus_tag="ACG842_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002387932.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
     gene            2666..2881
                     /locus_tag="ACG842_002360"
                     /pseudo
     CDS             2666..2881
                     /locus_tag="ACG842_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397514.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            3019..3990
                     /locus_tag="ACG842_002361"
     CDS             3019..3990
                     /locus_tag="ACG842_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFQ0248868.1"
                     /translation="MKTTMKDVAALAGVGVGTVSRVINGVKVKEVTKEKVMAAISELN
                     YEPDEYARGMKTNSSNTIALILPTIWHPFFSEFAYYVEEALSQKNYKLFLCNANGSAE
                     KEKEYIQMVKQSKVDGIIGITYSDIDKYVSSNLPFVSIDRHFSEQVSYVTADNYLGGQ
                     IAAQELLTRNCKNLCYVGGVSPYPNETNNRKAGFYNICEENKIAVKVLDMPEPISNLE
                     EQLLAFFSDNPEIDSIFTINDFMALDVIKAQKKLGKEAPKDFQIIGFDGVKLSLQKDY
                     LLSTIVQPIEEMAKVSVETLMAMILNEEPPKRVVVPVFFHAGETTKE"
     gene            4130..5443
                     /locus_tag="ACG842_002362"
     CDS             4130..5443
                     /locus_tag="ACG842_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HFQ0248869.1"
                     /translation="MKKRMLAIAAILALTAGGLAGCGSKEKSSGSSDPGEIKIWVQFS
                     DETPEGKAWAEIVNNFNDEYEGEYKAVTEYIPRSGSGGGYEDKVNAAITTNTLPDVIT
                     LDGPNTAAYAHSGVIAPLDDYLKDADMDDVLDSIIQQGTYDGKMYAFGFSESSVGVYY
                     NKSLFKDAGITEDQLPTLEDPWTWTEFQAICQKLTDKYKTPAIDMQMASGDEMLTYAY
                     TPFIWSNGGDVVNADGTKAEDYFNGKKSVEALEFIQELVEKGYTTISPIEKGFETGKY
                     AMMLSGSWTMADLEENYQEIDYGILPYPVSDATGELVSPTGSWQLAMTEKTEKKDASA
                     AFIKFATSTESSRIMSLGNSVLPIRNSTVDLIKDEVSEGMQVLIKQNQLSGHARPVVV
                     AYPQVSRAFQQAVQDISYFEENPDVQKVADSRAAEMQQAIDTSLK"
     gene            5486..6352
                     /locus_tag="ACG842_002363"
     CDS             5486..6352
                     /locus_tag="ACG842_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0248870.1"
                     /translation="MKDNLIGYGFLAPALLLLTIFLIIPVIMVIYYAFTDYYLLTPDA
                     RQFIGLENFKRLFADPIFVKSIWNTAKFVVWIIPVQIGAALGMALIINKNRKGNIFFK
                     VAFFAPVVMSLVVISILWLYLLNPNEGLVNTLFAKIGIASQPFLTSPKQAMYTLVFVS
                     AWQGAGYQMLLFLGGMQNIPQDVYEAAEIDGFSKFQQFRYITMPLLKPTAIFILLTTL
                     ISAFKLIIQPMVMTQGGPMNSTMTMVYYIYQTGFTDRMVGYSSSIALIFTTMIGLITI
                     AQRKLVKEDDPA"
     gene            6349..7197
                     /locus_tag="ACG842_002364"
     CDS             6349..7197
                     /locus_tag="ACG842_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0248871.1"
                     /translation="MTKKFGPLMIVEYCLLFLLAALFIFPMLWMVVSSMKPEADVYQN
                     LASFKAFLPSLNPANWFKTYQEVISRFSVGTYLLNSIFYGLTFACGSIIVNSLAGFAF
                     AKVNFSGKKIIFGALLALLIVPMETVLIPQFTIVNSLGLVNTRLAVILPAMASAFNIY
                     LFRNFFIAIPEEIIESAKLDGANIVKIFFHIMLPMSKPAIATVGVLSFISSWNDYIWP
                     LMVLTDSSKFSMQVAITTINTTQPVYINQVMAVLTIATIPLIIVYIVAQKYILQGLGG
                     SGTGIK"
     gene            7211..8683
                     /locus_tag="ACG842_002365"
     CDS             7211..8683
                     /locus_tag="ACG842_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HFQ0248872.1"
                     /translation="MLQKLLKANEFIEKNAQTVNQEYRNKFHLMAPIGWMNDPNGFVY
                     YQEEYHLFYQYYPYDSIWGPMHWGHAKSKDLIHWEHLPVALAPGESYDKEGCFSGSAI
                     EKDGKLYLIYTGHNVVEGQVRQVQCLAVSEDGIHFEKYAGNPIIAEDHLASVATTEDF
                     RDPKVFQRKEDYFTVVATKTADARGQIVMFHSKDLLTWEFYSVLLEGTPEQGIVWECP
                     DLFELDGKEVLILSPIQMTPQGNAYQNTNSTVAFIGTVDWQTGKFLVENFHEIDGGLD
                     FYAPQTCENEAGQRIMIAWMQMWQRKIPTNDLGHGWSGSMTLPRELRVADNQLLQRPV
                     SSIYQVIDPQERIEWAKTEENRFLLKDLVYDQHYLRLEINLKQATKLELHYAKGPDHT
                     LLLNYDVERECFTASREHMPYDLSGAEKEVLKKRSVTIPLENNCLTIEIFRDTSAIEL
                     FLNDRLTMSTTFYETEKGQDFLLLADSEVTFSLESGLINL"
     gene            8700..>8942
                     /locus_tag="ACG842_002366"
     CDS             8700..>8942
                     /locus_tag="ACG842_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFQ0248873.1"
                     /translation="MIYGSIEAGGTKFVCAISDEEMTIKERVSFPTTTPEETIPLVID
                     FFKQYQADLAGIGIGSFGPIDIHRDSATYGYITSTPK"
BASE COUNT         2830 a         1515 c         1831 g         2766 t
ORIGIN      
        1 atttaaaaag aaaagtgaca gataggaata aaaacttatt aaatgtagtt atgatctgtt
       61 atatttcatt tctaatacta ggtcttccac aagcagtgtt tcctgaaatg ttttctggag
      121 actggggtat tgtcagctat tctatattaa cattgattgc tatggtttta ggtacttcat
      181 tttataatgc ttttgtaagt aagcatgttg aggaatatga agtattaatg tacttaaaag
      241 agaaaaaaac aagtaattaa tatgagaaca ccacgcatag tttttatttt gagatattct
      301 agaatgaaac acattaagaa cgaaagcaaa gcaaaagacg ccagaacttc tactaccttc
      361 taagtttttt catatttgtt tgttatgcta tacatagatc tgcataatta gacttgcgct
      421 gcccgttatt gaagtttcag gcagcgctct ttttggggga tcgataatgg aagaagaaaa
      481 aatatttgag aaacgctggc agctagcatc aagtgaacaa agagcaagat acaataattt
      541 gatcacctcc taccccactg ttgactagac atataaggaa aagaaatact tactttggct
      601 gtgccagtta gatatagata cctttgaaac atttgaagtg atcctagata aaattaaaca
      661 gaattgatca gaaacaagcg aagccttctc tcggttcgct tgttttgcta tttattagtt
      721 ggctcagggc aactctaagc tcgtttgaaa gaaaaccgat ggataatccc cttgtttgta
      781 ccactttaga atacatggat gtacgtattg ccattgactc ctattagtta ctgaaaaaaa
      841 atttaaacaa gttgagtatt gcagataaat agctccagtg aggtgaataa ctgctccact
      901 caaggcaata gatagctcca tccagggcaa caaactgctc cactttcata tttaatagat
      961 gaataacctt ctgtataata gaaaggcata cctttcggta tgacaactta tacagaatgt
     1021 tattttattg gttcactatc gaaaaatttt ggagctttat ttttccggta tttctcaacg
     1081 aacgattagt ttaactgtgg gaagctccag acatacgatt cgagaagtta cttctcgagc
     1141 agacaggaag ggggtgactg aactaaccgc tgatatgagc gatacttggc tgtaggaata
     1201 tctttttccg gaaaaacaaa caacagcacg aggttacctt cctgtagact gggaatatgt
     1261 tcataaagaa ctaatgaaaa agaatatgac attgaaactc cttcatagag aatatggggt
     1321 acatggaaca tagaaacgaa aatgcacggt agaaaagcga atgaatatag tataaattct
     1381 acaaatcatt cgctttcttt attttatata tggttggtcg agagagacca ttttctttgg
     1441 caatttctgc aactggaact ccttgattta acatgtccac cacagtttta aagactaatc
     1501 gtttttgcgg atctgttgag ttctcagaat attcaattgg tcgcccctta tatcgaccag
     1561 cttttttggc caataatact ccttgccgct gacgttcacg gattttctca cgttcgtttt
     1621 gtgcaatata acttagcaat gataaaaaca tatcgaacat agcagtatct aggagttcat
     1681 ttccagtatt aaaatctaaa aactgtgcat ctagtatttt taatgctact tttttctgtt
     1741 tcataaaggt aactgtattt tttatgtctt cataatctct gcctaggcga tccagtgatg
     1801 caacaatgac acaatcacct tctcggatat aatttagtaa tttttgaaac tcaggtcgct
     1861 ggatatcttt accactggct ttttcttgga ataatttatc aatgttaagg tcttccatca
     1921 ttgccaattg tcgatctaaa ttttgatctt gtgaagacac tctaatatat ccaatttttg
     1981 ccataaaaac tccttttaaa aaatgatata atttacatgt aaaatttact tgtagtttta
     2041 ctgttatcat agcattttta aaaataaatt ttaagtctac ttataattta ctttggaggt
     2101 tttgaatgaa tgaaattaag cgcaataaaa attcttttgg tgtttattta ggagagccta
     2161 gctcggaaca gttaaataaa ggtgaagctc gtcatgctct atgtcgaaat atattttatg
     2221 gtagaaaagg caaactttat caaacttatt acgacggtat ggaggaacag ctcaacgcat
     2281 taggattagt aactaatgct atcatatatt ggaattcact atatttagaa aaagtctttg
     2341 aacaaattaa aacggaaggg ttcgactgtt ccgaagaagt gttacgcaag caatttccat
     2401 taattagtga tcacattaat tttgttggca aatatagttt ccgctatgaa aaagaattat
     2461 cgaatggaat actacgtccg ttaaatatag agaattattg agaatgttcg tcttttggtt
     2521 cctaccgtgc attttcgcac ggatgttcca gatactattt ttgaatgtat aaaattgaaa
     2581 acacaagaat actaataaat caacgattct tgtgttttta atttttcact accgtgcatt
     2641 ttcgtttcta tgttccatgt accccaatct tgaaaaatta attaaagact tagataaagc
     2701 cattgacgac ttagtcgtgg tgctaccgga gtatcaatgt ttaaccagta tcccaggtat
     2761 tggtcctgtt taggctgctg gaatactcgc tgaaattggt ccaattgaac gtttttctga
     2821 tgaaataaaa ttgacaaagt atgccggcct atattggcga cagagtcaat ccggtaaatc
     2881 tgaatacgaa aattttttta cgaaaagctt cacgtctaat atctatttta aaataaaaat
     2941 attcctgcta atttggaatc gtttccttct tttggtataa taatacctga aattcttttg
     3001 agaggagttg ttcgtgaagt gaaaactact atgaaggatg ttgctgcgtt agcaggagtt
     3061 ggtgtgggta cggtatctag agtaataaat ggagtaaagg tgaaagaggt taccaaagaa
     3121 aaagtaatgg ctgcaattag tgagcttaat tatgagcctg atgaatatgc cagaggtatg
     3181 aaaacgaata gtagcaatac aatagctctt attttaccta cgatttggca tcccttcttt
     3241 tccgagtttg cctactatgt agaggaagct ctcagccaaa aaaattataa gctttttttg
     3301 tgtaatgcga acggcagtgc ggaaaaagaa aaagaatata tccaaatggt caagcagagt
     3361 aaagtagacg gaattatagg tattacctat tcagacattg ataaatatgt atcatcaaat
     3421 ttaccatttg tcagtataga ccgccatttt tcggaacagg tttcttatgt aacggcagat
     3481 aattatttag gtgggcaaat tgccgctcaa gaactactta ctcgtaactg taaaaacttg
     3541 tgctatgttg ggggtgtttc accttaccct aacgaaacaa acaatcgtaa agctggtttt
     3601 tacaatattt gtgaggaaaa taaaatagct gtgaaggtat tggatatgcc tgaaccaatt
     3661 agcaatttag aggaacaatt gctcgctttt ttttcggata atcctgaaat tgatagcatt
     3721 tttaccatca atgatttcat ggctttggat gtgataaaag cccaaaaaaa actagggaag
     3781 gaagcaccta aggattttca aattattggt tttgatggag tcaagctatc gttgcaaaaa
     3841 gactatttac tatcaacgat tgtacagccg atagaggaaa tggccaaggt ttcagtagaa
     3901 acactcatgg cgatgatttt aaatgaagaa ccgcctaagc gtgtggtagt accggttttc
     3961 tttcacgctg gagagacgac taaagagtaa aaaaccggtt tataacgatt tttttgagat
     4021 aggttgacag aaagtgattt caatgatatc ttaaaaccaa ttaattggaa acgttaccaa
     4081 ttaattgaaa aataaaacgg aaacgtttca aaaaatagga ggaacatcga tgaaaaagag
     4141 aatgttagca attgcagcaa ttttagcatt aacagctgga ggacttgcag gatgtggcag
     4201 taaggagaaa agttcaggta gcagtgatcc aggagaaatt aagatttggg ttcagttttc
     4261 tgatgaaacg cctgaaggaa aagcttgggc agagattgtg aacaatttta atgatgaata
     4321 cgagggtgaa tataaagctg ttactgagta tattcctcgt agcggaagcg gcggtggata
     4381 tgaggataaa gtaaatgctg caattacaac caataccctt cctgatgtta ttacgttaga
     4441 tggaccgaat actgcagctt atgctcattc aggagtcatt gctccattag atgattattt
     4501 gaaagatgcg gacatggatg atgtattaga cagcattatt cagcaaggaa cctacgatgg
     4561 caaaatgtat gcgtttggtt tctcagagtc cagtgtgggt gtctattaca ataagtcgct
     4621 gttcaaagat gctggaatta cagaagatca attgccgacg ttagaagatc cttggacttg
     4681 gacggaattt caagcaattt gccaaaagct gactgataaa tacaaaacgc ctgcaattga
     4741 tatgcaaatg gcgtcgggag acgaaatgct gacctacgca tatacaccat ttatatggtc
     4801 gaatggcggc gatgtcgtta atgctgatgg aaccaaagcc gaagactatt ttaacggtaa
     4861 aaaatccgtt gaagcgttgg aatttattca agaattggtt gaaaaaggtt acaccacaat
     4921 cagtcctatt gaaaaaggat ttgaaactgg caaatatgcc atgatgctga gcggttcttg
     4981 gacaatggca gatttagagg aaaactatca agagattgat tatggtattt tgccttaccc
     5041 cgtttcagat gcaactgggg agttagtttc accaaccgga agctggcagt tagcaatgac
     5101 tgagaaaaca gagaaaaagg atgcttctgc ggcctttatt aagtttgcaa ccagtacaga
     5161 atctagtcgt attatgagtt tagggaacag tgttctacct atcagaaatt caacagttga
     5221 tttaattaaa gatgaagtgt cggaaggaat gcaggtatta attaagcaaa accaactttc
     5281 tggacatgcc cgcccagtgg ttgtagctta tccgcaagta tctagagcat tccaacaagc
     5341 agttcaagat attagctact tcgaggaaaa cccagatgta caaaaagtag cagatagtcg
     5401 cgctgctgaa atgcaacagg caattgatac ttcactaaaa tagaagtttc taaagggggc
     5461 caatcaagtg ttaaaaaaac cgacaatgaa agacaatttg ataggttacg gctttctagc
     5521 gccagctttg ctgctgctga cgatttttct gatcataccc gtaatcatgg taatttatta
     5581 tgcctttact gattattatt tgttaacgcc agatgctaga caatttatcg gcttagaaaa
     5641 ttttaagcga ctgttcgctg atccaatttt tgtaaaaagt atttggaaca cggctaaatt
     5701 tgttgtttgg attattccgg tccaaatagg ggcagcttta ggaatggcac ttattatcaa
     5761 caaaaatcgt aaaggaaata tcttttttaa agtagccttt tttgcgccag tcgtgatgtc
     5821 cttagtcgtt atttcgattt tatggttgta cttgttaaat cctaatgaag gtctggttaa
     5881 tacgctattt gcaaagattg gaattgcttc gcagcctttt ttgactagtc ccaaacaagc
     5941 aatgtatacg cttgttttcg tatcggcatg gcaaggtgca ggatatcaga tgctgctgtt
     6001 tttaggggga atgcaaaata ttccgcaaga cgtttatgag gcagccgaaa ttgatggttt
     6061 ttctaagttt cagcaatttc gctatatcac catgccatta ttaaagccca cggcgatttt
     6121 tattttactt accacattga tttcggcctt taaactaatc attcagccaa tggtaatgac
     6181 tcaaggtgga ccaatgaatt ctactatgac aatggtttat tacatttacc aaactggatt
     6241 tacagaccgt atggtaggct actccagctc cattgcgtta atctttacca ctatgattgg
     6301 tttaattaca attgctcaaa gaaaattggt aaaggaggat gatccagcat gaccaagaaa
     6361 tttggaccgc taatgatcgt agaatattgt ttgctgttcc ttttagcggc attattcatt
     6421 ttcccgatgc tgtggatggt agtttcctct atgaagcctg aagcagatgt ctatcaaaat
     6481 ttagcttcct tcaaagcttt tttacctagt ttgaatccag ctaattggtt taaaacttat
     6541 caagaggtta tttcccggtt cagtgtggga acttatttat taaacagtat tttttatggt
     6601 ttaacctttg cctgtgggtc aatcattgta aactctttgg cgggttttgc ctttgcgaag
     6661 gttaattttt caggcaagaa aattatcttt ggagctttgt tagctctgct gattgtcccg
     6721 atggagacgg ttttaattcc gcaatttacc atcgttaatt cactaggctt agtgaatact
     6781 cgcttggcag tgattttacc agcgatggcc agcgcgttta atatctatct gtttagaaat
     6841 ttctttatcg cgattccaga agaaatcatt gaatcagcga aattagatgg tgcaaatatt
     6901 gttaaaattt tctttcacat catgctgcca atgtctaaac cagccatagc cacagtaggt
     6961 gtcttatcct ttatctccag ctggaatgat tatatttggc cgttaatggt cttaactgac
     7021 agcagcaaat tttcaatgca agtggcaatt actacgatca atactacaca gccagtttat
     7081 atcaatcaag tgatggctgt tttaaccatt gcaacgattc cactgattat tgtttatatc
     7141 gttgcacaaa aatatattct gcagggactt ggcggttctg gaacgggtat aaaataggag
     7201 tgaatagtga atgttgcaaa aacttttaaa ggccaatgag tttattgaga aaaatgctca
     7261 aacagtcaat caagaatacc ggaataaatt tcatttgatg gctccgattg gctggatgaa
     7321 tgatccaaat ggttttgttt attatcaaga ggagtatcac ttgttttatc aatattatcc
     7381 ttacgacagc atctggggac caatgcattg gggccatgct aaaagcaagg atctaattca
     7441 ctgggagcat ttgccagtag cactggcacc aggtgaaagc tatgacaagg aaggctgttt
     7501 ttccggcagt gccatagaaa aggatggcaa gctgtatttg atttatacag gacacaatgt
     7561 tgtggaaggg caagttcgac aagttcagtg tttggcagtt tcagaagatg gcattcactt
     7621 tgaaaaatat gccggaaatc caatcatcgc agaagatcac ttagcttctg ttgcaacgac
     7681 cgaagatttc cgcgatccca aagtgtttca gcgaaaagaa gactatttta ctgtagtcgc
     7741 tactaaaact gcagatgctc gtggacaaat tgtgatgttc cattccaaag atttactaac
     7801 ttgggagttt tattccgtat tgctggaggg aactccagaa caaggcattg tatgggaatg
     7861 cccagacttg tttgaactag atggcaaaga agtcttgatt ctttctccta ttcaaatgac
     7921 tccacaaggc aatgcttacc aaaatacgaa ctcaactgtt gctttcatcg gtactgtcga
     7981 ttggcaaaca ggcaaatttc tagtagagaa ttttcatgaa atcgatggcg gtttagattt
     8041 ttatgcaccc cagacctgtg aaaacgaagc aggccaaaga ataatgattg cttggatgca
     8101 aatgtggcag cgtaaaatac ctacgaatga tctgggccat ggttggtcag gttccatgac
     8161 cttgcctaga gagctgaggg tggctgataa tcaattgttg caacggccag tttcatcaat
     8221 ttatcaagta attgatccgc aggaaaggat tgaatgggcg aagactgaag agaatcgctt
     8281 cttactgaag gacttagtat atgaccagca ctatttacgc ttggaaatca atttaaaaca
     8341 ggcaacgaaa ctagaacttc actatgcgaa gggacccgac catacattac tgcttaacta
     8401 tgatgttgaa cgggagtgct ttactgcttc cagggaacac atgccgtatg atttatcagg
     8461 agctgaaaag gaagtgttga aaaagcgcag tgtgacgatt cctttggaaa ataattgtct
     8521 aaccatcgaa attttccgtg atacttcagc tattgagctt tttctaaatg atcgtctgac
     8581 tatgagtaca actttttatg agacagaaaa aggacaggat tttcttttgc tggcagacag
     8641 tgaagtaacc ttttcattgg aaagcggctt aattaatcta tagaagggag agctcagtga
     8701 tgatttatgg ttcaatcgaa gcaggcggca caaaatttgt gtgtgccatt agcgatgaag
     8761 aaatgacgat caaggaaaga gtgagctttc cgacaacgac acctgaggaa acaatacctt
     8821 tagtcattga cttcttcaaa caataccaag cagatttagc agggattggg attggctcat
     8881 ttgggcctat cgatatccat cgcgattccg ccacttatgg gtacattacc tcgaccccga
     8941 aa
//
LOCUS       DAZKLM010000102         8849 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3764
            SAMEA115706913-rid22582563.denovo.106, whole genome shotgun
            sequence.
ACCESSION   DAZKLM010000102 DAZKLM010000000
VERSION     DAZKLM010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706913
            Sequence Read Archive: ERR13196647
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8849)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8849)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287823.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287823.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,004
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,849
            CDSs (with protein)               :: 2,849
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 41 of 96
            Pseudo Genes (incomplete)         :: 52 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 15 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8849
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706913-rid22582563.denovo.106"
                     /isolate="VRE3764"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(133..589)
                     /locus_tag="ACG842_002367"
                     /pseudo
     CDS             complement(133..589)
                     /locus_tag="ACG842_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035543.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
     gene            754..3116
                     /locus_tag="ACG842_002368"
                     /pseudo
     CDS             754..3116
                     /locus_tag="ACG842_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625383.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase"
     gene            3484..4407
                     /gene="truB"
                     /locus_tag="ACG842_002369"
     CDS             3484..4407
                     /gene="truB"
                     /locus_tag="ACG842_002369"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HFQ0248874.1"
                     /translation="MDGLLPLWKERGMTSHDCVFKLRKILHTKKIGHGGTLDPDVDGV
                     LPICIGKATKVIEYLTDSGKIYVGEITLGFSTTTEDRSGERVETKPVDMSLSEKKIDE
                     VMASFIGEITQIPPMYSAVKVNGKRLYEYARNNETVERPVRKAQIYRFIRTSELTRDE
                     VTGTISWRFEVECGKGTYVRTLAVDTGKKLGYPAHMSDLTRTASAGMSQKDALTLEEV
                     AQAVKNGTIDDCLLPIEKGVERFKRVDITDEVWQKVKNGMRLDYTVFALPEMPSEEIA
                     LFYKGKVVSIYQPNPKEKNKLKPSKVLRNEE"
     gene            4411..5352
                     /gene="ribF"
                     /locus_tag="ACG842_002370"
     CDS             4411..5352
                     /gene="ribF"
                     /locus_tag="ACG842_002370"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006747 - FAD biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009398 - FMN biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HFQ0248875.1"
                     /translation="MNIIKIRHPYQSEQIPEDDVVLVLGFFDGVHIGHQKVIKTGMEI
                     AQKEGLKLALMTFNQHPSIVFKKIDPSSVKYLTSLKQKEEKMAALGIDYLYEVDFTSS
                     FAHLAPQEFVDQYIVGLHAKYAVSGFDYTYGPKDIADVAHFPEYAKGRFKIVTVSKEE
                     EQGEKISSTRIRGLLDSGQVKEAAKLLGCTYEVEGVVVHGDARGRLLGFPTANVKVKS
                     TVHLPKEGVYVSEIKIGETWYPAMGSIGHNDTFGEGRELTVELYILDFNQDIYGEHVA
                     VRWNQFLRDQVKFSSVEELIEQLKEDEQATAEYFSQQ"
     gene            5704..6489
                     /locus_tag="ACG842_002371"
     CDS             5704..6489
                     /locus_tag="ACG842_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFQ0248876.1"
                     /translation="MYKELENKVAVVTGGSKGIGTAIAQRFGKEKMNVVVNYNSDKEG
                     AEKAVKAIEEAGGKAVSVQADVSSEEGIQRLLDTALENFGTLDVWVNNAGMENQHPTH
                     ELSLEDWERVLKVNLTGVFIGTKAALNYFTENNKKGNIINMSSVHEQIPWPTFAHYAA
                     SKGGVKLFTETVAMEYAPKGIRINSIGPGAIRTPINAAKFDDPEQKELLESMIPLGRV
                     GEPKEIAATAAWLASDESSYVTGITLFADGGMTLYPSFQGGKG"
     gene            6502..7353
                     /locus_tag="ACG842_002372"
     CDS             6502..7353
                     /locus_tag="ACG842_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015144 - carbohydrate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GRP family sugar transporter"
                     /protein_id="HFQ0248877.1"
                     /translation="MGLLIALIPAVGWGIQPLILKKIGGRPTNEILGTGIGAVLVGLL
                     VQLFASPAGISVTTFLISLLSGAFWVIGQTGQYTSFNLIGVSKTMPISTALQLVGTSL
                     IGVFAFGEWSGSMGKIIGAAAIILLVFGSALTAISDDPGKKGGITKGISLLASTSIGY
                     WVYSALPKLVDASGLSIFFPQMIGVFLGAVIYVIFRKPTAFSEDKSWKATIVGAIFSV
                     SALAYIFSANENGVATAYIITQLNVVISTLGGMYILHENKTPKEKKLTIIGLLLIVVG
                     SMVTVFI"
     gene            complement(7477..7929)
                     /locus_tag="ACG842_002373"
     CDS             complement(7477..7929)
                     /locus_tag="ACG842_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0248878.1"
                     /translation="MKDFYSVNELAEQLGVTTRSIRNYLHEGKLKGTKVGGQWKFSER
                     NLFEFLYGDQAEEAAKEMQRFMLNAPITMRFNLQYRDFTAINQFREQLVQYHNDVYAN
                     KKDRLLQYDLYKDNHAEILIGGNFNYVVNFSQWINEKLLIQTDISLVS"
     gene            complement(8394..8762)
                     /locus_tag="ACG842_002374"
     CDS             complement(8394..8762)
                     /locus_tag="ACG842_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HFQ0248879.1"
                     /translation="MEKFQPIINQIKEKEHQQKIKDLFAKITETYPQLDTTVKWNQPM
                     FTDHGTFIIAFSFAKNHFSIAPEKAAIRALEKNIQEAGYVYTDNVIKVPWKSVINWEL
                     IEQLISFNIEDKKGHDKFWR"
BASE COUNT         2973 a         1489 c         1837 g         2550 t
ORIGIN      
        1 aaatcagggg ttgcgggatt tttttgtttt cattaagacg tattacctaa gagttccatg
       61 actttagtct tgcggcgtgt cagctaacca aaaaagagaa aaagaaaagt aaaaaattcg
      121 actttctttt ttctagtagt atataaattt ttgcatatag ataaagatca agataccgat
      181 aacggaagct agattgtaat ctattttagt gacagtcagg tatagccaag taactagaag
      241 atcagtggaa cctgcaccat aaaagtttga gtttgtcggt ccgttgacag ttaaaagaaa
      301 aatcacgttg aagttattga tatttccgat tacgccgctt cgatttggtc acgatccaaa
      361 ttttggataa tccctgtaga aattagcata gtagctggga taccaattca catattgaca
      421 aagataacag agacttttac ataaaacgga tcaatcagga aaggaattgc tcgatccagc
      481 cccagttttc cagcaatgtg ttaactggcc cagcgtcatt cagcaaatta tgcatgatga
      541 ggagcgaaat gaactgcgga acagccattg tgataacgac aagttttttg gatcaatgta
      601 aaaaacgtca gcgaaaggaa aaatttcgtg gttttttata cctgttcatt tggctagata
      661 taaaatccaa acaaatgaac agaagtgctt attaggtttc aattgggagt atctgcgcta
      721 gaataagagt aataatgaaa aggagtgtta tgtatggatt attctagtaa agaatacttt
      781 gataaaatga cggcttggtg gcgtgctgct aattatcttt ctgttgggca actttatctt
      841 aaagataacc ccttactgag acgtacactg aaacccgaag atgtcaaaaa acatccaatc
      901 ggtcattggg gaacgattcc tggccaaaat tttatttatg tccatttgaa ccgagtcatc
      961 aataaatatg atttgaacat gttttatatt gaagggccag gacacggcgg acaagtcatg
     1021 gtatcgaatg cttatttaga tggaagttac acagagatct atccagaagt aacggaagat
     1081 gagacaggaa tgcaaaaatt attcaaacga ttttcatttc ctggaggcat tgcttctcat
     1141 gcagcaccag aaacaccagg ttctatccat gaaggcggag agcttggata ctctctttca
     1201 catgcagtcg gagctgtatt agacaatcct gaagtaatct ctgctgtcgt gattggtgat
     1261 ggtgaagcag aaaccggtcc tttagctgga agctggttct caaatgtctt tatcaatcca
     1321 gtgatagatg gagctgtttt accgatcctt catttaaatg gtgcaaaaat agccaaccca
     1381 acgatcttgg cacgtaaatc agatggagaa ttagcaaatt actttaacgg acttggctgg
     1441 gaacctttct ttattgaagg aaatgattca gaaaaattaa atcctgttat ggctgaaaaa
     1501 atggatcaag caatcgagaa aattaaatcc atccaaaaag aagctcgcct gaagacagca
     1561 acagatgtcg tgatgccaaa atggccagta ttgatcgtac gcacaccaaa aggatggacc
     1621 ggtccaaaag aatgggacgg ggaaccaatt gaaggaacct tccgagcaca ccaagtaccg
     1681 attcctgtag atcaagaaca tatggatcat gcagatgctt tactccgttg gctgaaatcc
     1741 tatgaaccag aaaaattatt tgatgcacaa ggacgtattt tagaagaaat cagagaaatc
     1801 gcgcctactg gtgaccaaag aatggcaaaa aatcctatca ccaatggtgg aatcgatcca
     1861 agaccgctta ttatgccaga ttggaaaaaa tatactttgc aatttgaaaa accaggctcc
     1921 gttaaagcag aagatatgac cgaacttggt aaattcgttc gcgagatcat tgagaaaaat
     1981 ccagaaaatt tccgtatatt tggaccagat gaaacaaaat caaaccgatt gaatcaagtt
     2041 ttcaaaacga caaatcgcca atggatggaa aaaatcgaac cagaaaatga tgaatggctt
     2101 tcgccatctg gtcgcgtcat cgacagtcaa ttatcggaac accaagatga agggttctta
     2161 gaagggtacg ttctgactgg acggcatggt tttttgcaag ttacgaatca ttcttgcgag
     2221 tggttgactc tatgttgact cagcacttca aatggatgcg gaaatcacat gacctttctt
     2281 ggcgaaatga ttatccttcg ctaaatctca ttgcaagctc aacagtcttc cagcaagatc
     2341 acaatggata tagccaccaa gatccaggaa tattgacaca tttggcagaa aagaaggcgg
     2401 agttcatccg tgaatatctg cctgcggatg caaatacatt gctcgcagtc atggataaag
     2461 cattccgctc gtcagaaaaa atcaatttga tcatctcttc caaacatccg cgtgctcaat
     2521 tttactcagc agaagaagca gccgtcttag tgaatgaagg cttgaaaatc attgattggg
     2581 cttcgacagc aaaagaggag gaacctgaat tggtcattgc tgctgcagga acggaatcta
     2641 acttagaagc attagcagct gtaactttgc tgctggaaga attcccaaaa ctaaaaattc
     2701 ggttcatcaa tgtcgtagat ttattgaaat tacgtcatcc aagtcaagat ccgcgtgggt
     2761 tgagcgatga agaatttgat caatatttta caaaagacaa accgatctta tttgctttcc
     2821 acggttatga aacattggtc cgcacgattt tctttgatcg tcataaccat catttaatga
     2881 ttcatggcta caaagaaaat ggagatatca cgacaccatt tgacatgcgt gtagtaaatg
     2941 aacttgatcg ttatcatttg gcaaaagacg cagctttaaa aatcaaagga agtcaagcag
     3001 aagattttgc gaaaaaaatg gatcagaaat tacaagaaca tcaaaactat attcgtgaaa
     3061 atggtattga tctgccagaa gtattggatt ggaaatggaa aaatcttgac caataaaatg
     3121 ataaaatcga tcccgcaaaa tagaattttt tacattgatt aagaaactga aaaaataaat
     3181 aaaaaaaacg gaaaaaagac tggaagaatg gccagtcttt tttttcgttt ttgggaagtg
     3241 aaaaccaaac tatttacctg taagccaccc ataaatccaa tcaaatggtg caaagaaaaa
     3301 atcttccatt ctattccctc ctttaaaaaa gcataacatg atttctattt acgtgagcat
     3361 gaattttgtt acgtttttat tgccgaaaaa taaaaaccgt tcattatgat gtcaaattcg
     3421 tcagtaattg atgcaatgtg ttataataat tcagttaaat caaaaatgaa atggagaaaa
     3481 gacatggacg ggttattacc tttatggaaa gaacgcggaa tgacaagtca tgattgcgta
     3541 ttcaaattaa gaaaaatctt acatacgaaa aaaatcggac atgggggtac gcttgaccca
     3601 gacgtagacg gtgtgttgcc catttgtatt ggaaaagcca caaaagttat cgaatatttg
     3661 acagattctg ggaaaatcta tgtaggcgag ataactctag gtttcagtac aacgacggaa
     3721 gatcgatccg gagaacgggt agaaacaaaa ccagtagata tgtctttatc tgaaaaaaaa
     3781 atagatgaag tgatggcttc atttattggt gagatcaccc agataccacc aatgtactca
     3841 gcggtaaaag tcaacggtaa gcggttatat gaatatgccc gcaataatga aacagtggag
     3901 cgtcctgtta gaaaggctca gatttaccgt tttatccgga caagtgaatt gacaagagac
     3961 gaagtgacag gaaccatttc ttggcgcttc gaagtagaat gcgggaaagg aacctatgtc
     4021 cgcacattag ctgtagatac aggcaagaaa cttggttacc ctgcgcacat gtctgatctg
     4081 acccgaacag caagtgccgg tatgtcccag aaggatgctc tgacattaga agaagtagct
     4141 caagctgtga aaaatgggac aatcgatgat tgtttgctgc caatagaaaa aggagtggaa
     4201 cggttcaaac gagtagatat tactgatgaa gtatggcaga aagtaaaaaa cggtatgaga
     4261 cttgattata ctgtatttgc gctcccagaa atgccgtcag aagaaattgc tttgttttat
     4321 aaaggaaaag ttgtcagtat ttatcagcca aacccgaaag aaaaaaacaa attaaaacct
     4381 agtaaagtat tgagaaatga ggaataacga atgaatatca tcaagatcag acatccttac
     4441 caatcagaac aaattccaga agacgatgtc gttttagtgc tgggattttt cgacggcgtg
     4501 catatcggac accagaaagt cataaagaca ggtatggaaa ttgcacaaaa agaaggattg
     4561 aaattagcat tgatgacttt caatcagcat ccatctattg tctttaaaaa gatcgatcct
     4621 agcagcgtga aatatttgac tagccttaag caaaaagaag aaaaaatggc cgctttgggt
     4681 atagattatt tatatgaagt tgattttact tcttcttttg ctcatttagc accgcaggaa
     4741 tttgttgatc aatacatcgt agggttacat gcaaaatatg ctgtttccgg ttttgattat
     4801 acgtatggac caaaagacat tgcagatgtc gctcattttc ctgaatatgc caaaggaaga
     4861 ttcaaaatcg tcacagtttc taaagaagaa gaacaaggtg aaaaaatcag ctctacccgt
     4921 attcgtggat tactagacag cggacaagta aaagaagcag caaaactatt aggttgcaca
     4981 tatgaggtag aaggggtagt tgtccatggc gatgcacgag gcagactact aggattccct
     5041 acagcaaatg tcaaagtaaa gagcacggtt catttgccta aagaaggtgt ttatgtgtcg
     5101 gaaataaaaa ttggggaaac atggtatcca gccatgggtt caattggaca taatgataca
     5161 ttcggtgaag gacgggagct gactgtagag ttgtatattt tggactttaa tcaagatatc
     5221 tatggtgaac atgtcgcggt acgctggaat caatttttaa gagatcaagt gaaattttct
     5281 agtgtagagg aattgattga acaactcaag gaggatgaac aagcaacagc tgaatatttt
     5341 tctcagcaat aaaacattat gcataaaatc tcttgtttat gtataactag caattacaat
     5401 aaaatgggtg ataaataagc ccaggaaagg ggctgtgaca aaagtcttgt cacagccttt
     5461 ttttccaaat aaatggtttt tcagaagtag cttcttcgga aataagccga aatttcttga
     5521 agctgaccac ttctgtcaca acctctttct ttatagaaat aaaaggacac tagcaattca
     5581 tgaaaacgat tgctagagcg tatcatttgc tttagactat atatcttggt gtacactcac
     5641 tttaaggcgg aaataaaagt tatccgtaat aacaggttta ggtatgaaaa aggagtgggt
     5701 actatgtaca aggaattaga aaacaaagtc gcagtagtaa ctggtggctc aaaggggatt
     5761 ggaacagcga ttgctcaacg attcgggaaa gaaaaaatga atgtagttgt caactataac
     5821 tcggataaag aaggagcaga aaaagcagtc aaagcaatcg aagaggcagg cggaaaagca
     5881 gtctctgtac aagcagatgt cagttctgaa gaaggaatac agcgtttact tgatacggct
     5941 ttagaaaatt ttgggacact ggacgtttgg gttaataatg ctgggatgga aaatcagcat
     6001 ccaacccatg aacttagcct tgaagattgg gaaagagtgt taaaagtcaa tttgactggc
     6061 gtttttatcg gaacgaaagc ggctttaaat tactttacag agaataataa aaaaggaaat
     6121 atcatcaata tgtcttctgt tcatgaacag atcccatggc caacgtttgc tcattacgct
     6181 gcttcaaaag gcggggtaaa attatttacc gaaacggtag caatggagta tgcaccgaag
     6241 ggaatcagaa tcaatagtat aggccctggt gctatccgta cacctattaa tgcggcaaaa
     6301 tttgatgatc ctgaacaaaa agaactcttg gaaagtatga tcccactagg acgcgtaggt
     6361 gaaccaaaag aaatagcggc gacagctgca tggttggctt cagacgaatc cagttatgtt
     6421 acaggtatta cactatttgc agatggtggg atgactttat atccttcttt ccaaggtgga
     6481 aaaggataag ggtgaataaa aatgggttta ttgattgcat tgatcccggc agttggctgg
     6541 gggatccagc cactgatttt gaaaaagatt ggtggcagac caacaaatga gatattagga
     6601 actggtattg gagcggtgct tgtcggctta ctcgttcaat tattcgcttc tccagctggt
     6661 atttcagtga ctacattttt gattagttta ctttctggtg cattttgggt tattggacaa
     6721 acaggacagt atacatcttt caacttgatt ggtgtttcta aaacgatgcc aatttcgact
     6781 gcattacagt tagtcggaac ctcgttgatt ggtgtcttcg cttttggaga atggtctgga
     6841 agtatgggga aaatcattgg agcagcagca atcattcttc ttgtatttgg ttctgcttta
     6901 acggcaatca gtgatgatcc tggaaaaaaa ggcggtatca caaaaggaat tagtctatta
     6961 gcatcaacaa gtattggtta ttgggtatac agtgcattgc ctaaactagt tgatgcaagc
     7021 ggactttcca ttttctttcc gcaaatgatc ggtgtctttt taggggcagt gatttatgtg
     7081 attttccgta aaccaaccgc attttctgaa gacaagagtt ggaaagcaac gatcgttgga
     7141 gccatcttta gtgtgtcagc attggcttat attttttcag caaatgaaaa tggggttgct
     7201 actgcatata tcattacaca gctaaacgta gttatctcta cattaggcgg tatgtacatc
     7261 ctgcatgaaa ataagacacc aaaagaaaaa aaattaacga ttatcggatt attgctgatc
     7321 gtagtaggaa gcatggtcac ggtctttatt tgaggataaa gatggtaaaa tttcatttat
     7381 taaaaaaata tttgtttgaa ccaaacagta agtacttata aaaaaatagc gctagaattt
     7441 ccaacatctc tggaatttct agcgcttttt aattgtttaa gatacaaggg aaatatctgt
     7501 ctggatcaat aatttttcat tgatccattg actaaagttt acgacataat tgaagttacc
     7561 accgattagt atctccgcat gattgtcttt ataaagatcg tattgtaaaa gacggtcttt
     7621 tttatttgcg taaacatcgt tatggtactg gactaattgt tctcggaatt gattgattgc
     7681 tgtaaaatca cgatattgaa ggttgaaacg catcgtgatt ggtgcattta gcatgaatcg
     7741 ctgcatttct tttgctgctt cctctgcttg atctccgtat aggaactcaa aaagattacg
     7801 ttcagaaaat ttccattgcc cgccaacctt tgttcctttt agttttcctt catgaagata
     7861 atttctgata gaacgtgtag taacacctaa ttgttcggct aattcattta ctgagtaaaa
     7921 atctttcaat gttcaccctc ctatgtgaat ggttctactg acttagttga tatttgctta
     7981 atcacgaaat gtagaaagaa cgattttaat taaggtcatt gtaaaaatca agtcagaaaa
     8041 ttctaacttt tatttctcta aagaaattcg ttctctccta aagtgatatt atatttaaat
     8101 atacctgaaa ataatgggca gtattatgaa agatcactga tattttctcg tattttttcc
     8161 ttattcaaaa gttttgcagg aaaattgctg aaaaaataat tttactaagt agaaaattca
     8221 aagccattca atcagtgatt tttttcagca caagaaagac tccttataaa aatagcagat
     8281 actttgtcaa ttaagtgttt aaccagggat gataaaaagg ataggattga acaaaacctg
     8341 tataaggtga tgaatagttt cctcgccagt aaaaggttta ttcgtataaa gctttatcgc
     8401 caaaatttat catgaccttt tttatcttca atattaaatg aaatcaattg ttcaatcaat
     8461 tcccagttga ttacactttt ccaaggaact ttgatgacgt tatccgtata tacgtagcct
     8521 gcttcttgga tatttttttc aagggcacga atcgctgctt tttccggtgc gatagaaaaa
     8581 tgatttttag caaaactaaa tgcaataata aaggtaccat ggtcagtaaa cataggttgg
     8641 ttccatttta cagttgtgtc tagctgggga tacgtttctg ttatcttagc aaaaaggtct
     8701 ttgatctttt gttgatgttc tttttctttg atttgattga tgatcggctg aaatttttcc
     8761 attctaaaac gcctccatta agtggtttga tttattttac cagaaaaaca atagttatgt
     8821 ataaaaaacg aacaaaacac aagtaatgt
//
LOCUS       DAZKLM010000103         8753 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3764
            SAMEA115706913-rid22582563.denovo.107, whole genome shotgun
            sequence.
ACCESSION   DAZKLM010000103 DAZKLM010000000
VERSION     DAZKLM010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706913
            Sequence Read Archive: ERR13196647
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8753)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8753)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287823.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287823.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,004
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,849
            CDSs (with protein)               :: 2,849
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 41 of 96
            Pseudo Genes (incomplete)         :: 52 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 15 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8753
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706913-rid22582563.denovo.107"
                     /isolate="VRE3764"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(351..2732)
                     /locus_tag="ACG842_002375"
     CDS             complement(351..2732)
                     /locus_tag="ACG842_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HFQ0248880.1"
                     /translation="MANEQTRSSRRQKQPTPKKSVKKNSGKGSGKSSGTHKKGLFIKI
                     LLGILSFFCILFLAGVGLFWYYAKDAPELTDKKLDATVSSKLYTQDGELFEDLGAEKR
                     EKISANELPKTLEDAIVSVEDRRFYKHIGVDPIRIIGSALSNFTSGGLQGGSTLTQQL
                     IKLSFFSTSAEDQTLKRKAQEAWMAVRLEQKKSKQEILTYYVNKVYMSNGLYGMETAS
                     EMYFGKKLSELSLPQTALLAGMPQAPSAYDPYVYPDQAKKRRDTVLYTMLQNEKISQT
                     EYDQAVNVPVTDGLQELTQSDDNTKIVDNYVKEVINEVQEKTDKNVYTDGLEIYTNLD
                     LDAQKKLYDIVNTDQYVSYPDDEMQVASTLIDTNTGKVKAQIGGRHIAEDVTLGNNLA
                     VNTSRDFGSTMKPVTDYGPAFEYLKYSTGKTITDAPYNYEGTSTPVGNWDNQYMGTIT
                     LRQALYLSRNVPAVKLFNEVGSDKVASFLKNLGIEYSTIHQSNAISSNTEEQDGTKYG
                     ASSLKMAAAYAAFANGGTYYKPQYVNKIVFQDGTEETYEPDGKTAMSPETAYMITDIL
                     KDTITEGTGTNAQIAGLYQAGKTGTSNYTDDEYAKLGISSGVYPDILFAGYTPNYSIS
                     VWTGYNKKMTPVTSESSHVASDVYRELMQYVSANVTNTDWEMPSGLIRVGGELYYKDQ
                     YTARSNAITPSTTIPSSSYVQTPGSSTTETTTQSSSSTSQSESTAESSKESTTAETSE
                     PASSTTVPSSSSEESSTPSSSAPPASSSEPATSGADAANDHTPSSSTSASGNR"
     gene            complement(2722..3369)
                     /gene="recU"
                     /locus_tag="ACG842_002376"
     CDS             complement(2722..3369)
                     /gene="recU"
                     /locus_tag="ACG842_002376"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HFQ0248881.1"
                     /translation="MVLRYPNGQPYYKNASPVEKIQEKRNQITEFGNRGMRFEEAINE
                     SNKYYLSRGLAVIHKKPTPIQIVKVDYPKRSAAVIKEAYFTQASTTDYNGVYRGFYLD
                     FEAKETKNKTSFPFKNFHAHQIEHMKACLEQNGVCFVLLWFSSLKRCFYLNSVHLIAY
                     WDAQKEKGRKSLPLSLIEELGIEIKTGISPRIPYLDAVDFYLSTLSIEGESKNGK"
     gene            3433..3972
                     /locus_tag="ACG842_002377"
     CDS             3433..3972
                     /locus_tag="ACG842_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HFQ0248882.1"
                     /translation="MDKSKVIYVSGYRSFELGVFKENDPKIQVVKNVLKKELHQLAEE
                     GLEWVLVSGNLGVELWAVQIVAELKEEYPELSLGILYPFSDFGNNWNEGNQEKKQLAE
                     QLADYVEAVSHQPYQSPSQLKNHTRFILEHTVGCLLIYDEEFPGKTKYFFEDAQNYQA
                     DHPYEIRLISMDDLQNFSE"
     gene            4062..4481
                     /gene="gpsB"
                     /locus_tag="ACG842_002378"
     CDS             4062..4481
                     /gene="gpsB"
                     /locus_tag="ACG842_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HFQ0248883.1"
                     /translation="MANLVYSPKDILQQEFKTKMRGYDPVEVDEFLDNIIKDYETYSK
                     ELLALQEENDRLSAKVAQLSKTQGAAQTRVQQTEAPKSAAVTNFDILKRLSNLEREVF
                     GKKLDQQASAVKPAQPNPNNYTNADTSLDDNEKTRQF"
     gene            4503..4870
                     /gene="rnpB"
                     /locus_tag="ACG842_002379"
     ncRNA           4503..4870
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="ACG842_002379"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            5079..6245
                     /locus_tag="ACG842_002380"
     CDS             5079..6245
                     /locus_tag="ACG842_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HFQ0248884.1"
                     /translation="MEKNRKFNLVATAASGLEALVGKELRDLGIECQVENGRARFEGT
                     METIATANLWLRTADRVKIVVGEFDAYTFDELFENVKALPWEDFLPLDAAFPVAGKSI
                     KSKLYSTPDCQAITKKAIVERLRTYYHRPASVPLTETGAHFQLEVALLKDHVMVTLDT
                     TGPSLFKRGYRLEKGGAPLKENMAAALIMLTNWRKDRPFYDPVCGSGTLCIEAALIGH
                     NIAPGFNREFACESWDWFSQEIMENVRSAAEEKADYDIELDITGSDINGRMIEIAKAN
                     AEEIGLGSSITFKQQAVKDFKTDKEYGVIVANPPYGERLGEEETVRKLYKEMGDVFRP
                     LKTWSKYILTSDLAFEEFYGQKATKKRKLYNGALRTDLFQYWGTRPPRKKEKDE"
     gene            6291..7787
                     /locus_tag="ACG842_002381"
     CDS             6291..7787
                     /locus_tag="ACG842_002381"
                     /EC_number="3.4.17.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HFQ0248885.1"
                     /translation="MKEKEFLQEVKEINLLQQALGILDWDTQTGMPEKASAYRSEVDS
                     YLYSIYFSKKIGPKIKEAIRYFSEHPEELSEVGTAVFAKVKEEYDLEHRVPEDLMRKY
                     TAAVSSAHGQWQKARETQQFSDFQHALNQNIELTKQLIPYWRKDEKTNYDVLLNQYEP
                     GMTVEILDKVFDQLKEGILSIRRTLQEKGTEPETDFLNRRMTKKQQKRFVVKVIEQLG
                     YDFSRGRLDDTVHPFMTGINPNDARITTRWNETDFKMAVFGVIHEAGHGMYEQDIDDK
                     YAYTPVYEGTSMGIHESQSLFNEIIIGSSRSFWKKQYPFFQECAEGTFDDIPFDKFYR
                     SLNHTKSSLVRIEADSLTYPLHIIIRYEIEKMIFNEGVDVAELPEIWNQKYEEYLGIR
                     PTNDVEGILQDVHWSGASFGYFPSYALGYMYAAQLLHSLQQEIDVENVLASDDYSPIK
                     NWMSEHIHQYGASRKPNQLIMDATHEPLNPQYLIDYMKDLYFNVYQVK"
     gene            7868..8551
                     /locus_tag="ACG842_002382"
     CDS             7868..8551
                     /locus_tag="ACG842_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFQ0248886.1"
                     /translation="MDKLQVIADYSMDQLAHDQTGHGSDHTKRVVKLAEKILETEPQA
                     DRFVTLAAAYLHDTIDDKVVKNENEAKQQLRVFLRSLPIVEEQISMIFSIIENMSFSK
                     NLSEAAELSLEGKIVQDADRIEALGAIGILRTAYFGGGHGHPIFDPELYPQTFKDKEN
                     YRKGTTVINHFYEKLFLLSDKMNTDYGYEEAKRRETFMREFLEEFFLEWSIDDENFTP
                     DNWIKNNKK"
     gene            8560..>8753
                     /locus_tag="ACG842_002383"
     CDS             8560..>8753
                     /locus_tag="ACG842_002383"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0248887.1"
                     /translation="MTEVVSSKIFRIIFRKSYFWNTVYQEVVSLPFSSESQGTISKNS
                     FSCLMNIVTCDSELAGEHRL"
BASE COUNT         2761 a         1583 c         1848 g         2561 t
ORIGIN      
        1 tactatcaga taactcaaaa tgaagccttc tttcaaaaaa ttattcaaaa ccatatattg
       61 tattttttat ttgataataa caatgggatt attaagaagt ggcactaaat aaaagactag
      121 tgtgtattta gccgatttaa ttataaacaa gtaaatggat aaagtatatg taatgataaa
      181 agaaaaatgg attttctaat ttttaagcta aatgttagat cacacatgta tatgctggat
      241 agtcttataa aaaatcatgt aatacagctt ggtacatgat ttttatttgt agctgttttt
      301 tacatagaca caaaaaagaa accacgcttg tagacgtggt ttctttttta ctatctgttt
      361 ccagaagctg aggtgctgct ggatggtgta tggtcatttg cagcatccgc tccgctagtt
      421 gctggctcac tggaagaagc aggaggagca gatgaagacg gtgtcgagct ttcttcggat
      481 gaagaagaag gaaccgttgt tgaagacgct ggctcgcttg tttctgctgt cgtgctttct
      541 tttgaggact cagcagttga ttcgctttga ctagtagagc tagagctttg agtcgttgtc
      601 tctgttgttg agcttccagg agtctggaca tagctggaag atggaatagt tgttgatggt
      661 gtaattgcat tcgatctagc tgtatattga tctttgtagt acagttcacc gcccacacga
      721 attaatccgc ttggcatctc ccagtctgta tttgtcacgt tagctgaaac atactgcata
      781 agctcgcgat aaacatcaga ggctacgtgt gacgattctg aagtgacagg cgtcattttt
      841 ttattgtaac ctgtccacac ggaaatcgaa taatttggcg tatagcccgc aaataggata
      901 tctggataca ccccagaaga aataccaagt tttgcatact cgtcatctgt gtaattggat
      961 gttccggtct taccggcttg atataaccca gcgatttgcg cattcgtacc agttccttca
     1021 gtgatcgtat cttttaaaat gtctgtgatc atataagctg tttctggaga catggctgtt
     1081 tttccatctg gctcataagt ttcttctgtt ccgtcttgga agacgatttt attaacatat
     1141 tgaggtttgt aataagtccc tccattggcg aaagccgcat aggctgctgc catttttaag
     1201 gaagaagctc cgtattttgt cccatcttgt tcttccgtat tactagagat cgcattcgat
     1261 tgatggatcg tactgtattc gatcccaaga ttttttaaga aagaagccac tttatctgaa
     1321 ccgacttcat tgaataattt gactgctgga acattacggg ataagtaaag tgcttggcgt
     1381 aaagtaatgg tgcccatata ttgattatcc cagtttccta caggagtcga agtcccttca
     1441 tagttatacg gtgcgtcagt aatagttttt ccagtagaat atttcaaata ttcaaaagct
     1501 ggtccgtaat ctgtgacagg tttcatcgta gaaccaaaat cacgggaagt attcactgct
     1561 aagttgttgc ctaaagttac gtcttctgcg atgtgacgtc cgccaatctg ggcttttact
     1621 ttcccggtat tggtgtcgat aagggtggag gcaacttgca tctcgtcgtc tgggtaagag
     1681 acatactgat ccgtgttgac gatatcatat agtttctttt gggcgtccag atccaaattc
     1741 gtataaatct ctaaaccgtc tgtgtagaca ttcttatctg ttttttcttg tacttcattg
     1801 atgacttctt tgacataatt atcgacgatc ttagtattgt catcggattg agttagttct
     1861 tgcagaccat ccgttaccgg tacattaaca gcttgatcat attctgtttg agagattttt
     1921 tcgttttgaa gcatcgtata caaaacagta tctcgtcttt tcttcgcctg atctggataa
     1981 acataaggat cataggcgga aggggcttga ggcatcccag caagcaatgc tgtttgcggt
     2041 aaagaaagtt ctgataattt cttaccaaag tacatctcag aagctgtctc catcccgtac
     2101 aagccgtttg acatatacac tttatttacg tagtaggtca agatctcttg tttcgatttt
     2161 ttttgttcca atcttacagc catccacgct tcttgggcct tgcgtttcag agtctgatcc
     2221 tcggcactgg tggagaagaa ggaaagtttg atcaattgct gtgtcaaagt acttccgcct
     2281 tgcagtccgc cagaggtaaa gttagagagg gcagaaccga tgatccgaat agggtcaacc
     2341 ccgatgtgtt tataaaaacg gcgatcttct accgacacga tcgcatcttc taatgttttc
     2401 ggtaattcat tcgcagaaat tttttcacgc ttttctgcgc ctaaatcttc gaatagttcg
     2461 ccatcttgtg tatatagttt ggaagatacg gtagcgtcta attttttatc cgtcaattcg
     2521 ggcgcatctt ttgcgtaata ccagaaaagt cctacacctg ctaaaaagag gatacagaaa
     2581 aatgagagta ttcctaaaag gatcttgata aataatcctt ttttatgtgt tccagaggac
     2641 tttccagaac ctttgccaga attcttcttc acactctttt taggagtagg ctgtttctgt
     2701 cgtctagagc ttctagtttg ttcatttgcc attcttgctt tctccttcaa tagataatgt
     2761 tgataaatag aagtcaaccg cgtccaaata aggaatccgc ggagaaatac cagttttgat
     2821 ctctattcct agttcttcga tcaaagataa aggtagtgat ttgcgtcctt tttccttttg
     2881 ggcgtcccag taagcaatca aatgaacact attcaaataa aaacagcgct tcaaagacga
     2941 aaaccataac aagacaaagc agaccccatt ttgttcgagg catgctttca tatgttcgat
     3001 ttgatgggca tggaagtttt taaagggaaa agatgttttg ttcttggttt cttttgcttc
     3061 aaagtccaag taaaagcctc gatagacgcc attatagtct gtagttgagg cttgggtaaa
     3121 gtaggcttcc ttgatcacag cagcacttct cttaggatag tctactttca cgatttggat
     3181 aggggtaggc tttttatgga taaccgccaa cccgcgggaa agataatatt tattactttc
     3241 attgattgct tcttcaaagc gcatcccccg attgccgaat tcagtgatct gatttctttt
     3301 ttcttgaatt ttttcgacgg gagaggcatt cttataataa ggttggccat ttggatagcg
     3361 caataccatc tttaatcaca ctcctaggcc acattatagc aaaaaagcgg atagaaagaa
     3421 aaggaatctg agatggataa gtcaaaagta atctatgtgt ctgggtatcg aagtttcgaa
     3481 ctcggtgttt ttaaagaaaa tgatcctaaa atccaagttg ttaaaaatgt tttaaaaaaa
     3541 gaactccatc agttagcaga agaagggctg gaatgggtac ttgtcagtgg gaatttgggc
     3601 gtcgaactat gggctgtgca aatagtagcg gaattgaaag aagaatatcc ggaactatct
     3661 cttggtattt tgtatccttt ttctgatttt ggtaataact ggaatgaagg gaaccaagaa
     3721 aaaaaacaat tagcagaaca attagcagat tatgtcgaag ctgttagcca tcaaccctac
     3781 caatcaccta gtcagctaaa gaatcacact cgttttatac ttgagcatac agtgggctgt
     3841 ctgctgatct atgatgaaga attccctgga aaaacgaagt atttttttga agatgcacag
     3901 aattaccaag cagatcatcc ctacgaaatt cggctgatca gtatggacga tcttcaaaat
     3961 tttagtgaat aatcgatatt gtgtttgatt tatcaacttt tttcctatac aatgaaaaag
     4021 gtagaaatgt tattacgtaa atgaaaacta gaggtgtaaa tatggcgaat ttggtttata
     4081 gtcctaaaga cattttacaa caagaattca aaacaaaaat gcgcggctat gatcccgttg
     4141 aagttgatga attcttagac aatatcatca aagattatga aacttacagc aaagaactcc
     4201 ttgctttaca agaagaaaac gatcgattga gtgcaaaagt agctcaatta tctaaaactc
     4261 aaggagcagc tcaaacacgc gtgcaacaaa cagaggcacc aaagagcgca gcagtgacca
     4321 attttgatat cttaaaacgc ttgtcaaatc ttgaacgtga agtatttggt aaaaagcttg
     4381 atcaacaagc aagtgcagtc aaaccagcac aacctaatcc aaataactac acaaatgcag
     4441 acacaagctt agatgataat gaaaaaacac gtcaatttta atttttgatt aatgaatttg
     4501 cggttttcgg gtaatcgcgg tctgacttga tcaggctgag gaaagtccat gctcgcacag
     4561 gctgtgatgc ctgtagtgtt cgtgcttagc gaaaccataa gctaaggtac tctttggagt
     4621 aacggcagga aaacaagcta aggcttcggc tatgcttaag tatccttgaa agtgccacag
     4681 tgacgaagcg gctggggaaa cccatccggt ggaacgcggt aaacccctcg agcgagcaac
     4741 ccaaacaatg gtaggggcgc tttttgtctg gaagaagaac aggacaaaaa ggcagtataa
     4801 ctgcagatag atggttaccg ctttgttttt tctccctgga aaagcaaagt acagaacatg
     4861 gcttatagaa aaccgcaaac atcagggaat taggtcgtga caaaagtaat gcgttataag
     4921 aaacaagaaa aacatttgaa agctattttt caatacttca gatgaatcgt tcttattaca
     4981 aaacaaactt ttgtatcacc ggttattcgg aatttgagta aaagtgtggg gtaggactga
     5041 tgtcccacct ccttttttta taaataagga gattgtgtat ggaaaaaaac aggaagttca
     5101 acctcgtcgc aactgcggca agcgggctag aagcactggt tggtaaagaa ttaagagatc
     5161 ttggaatcga atgccaagtt gagaacggac gtgcacgttt tgaaggaaca atggaaacga
     5221 tcgctacagc taatttatgg ttacgtacag cagatcgagt aaaaatcgtg gttggggaat
     5281 tcgatgcgta tacgtttgat gaattgtttg aaaacgtaaa agcattgcct tgggaagatt
     5341 ttcttccact agatgcagct tttcctgtag ctggaaaatc gatcaagtcg aaactatata
     5401 gcacacctga ttgccaagcg atcacaaaaa aagcaatcgt tgaacgtctc cgtacctatt
     5461 atcaccgtcc agcttctgta ccactgacgg aaactggcgc acattttcag ttggaagtag
     5521 ctttgctgaa agaccatgtg atggtaactc tggatacgac aggaccaagt ttattcaaac
     5581 gtggttatcg cttagaaaaa ggtggagcac cattgaaaga aaacatggca gcagcactta
     5641 ttatgttgac aaattggcga aaagaccgtc cattttatga tcctgtatgt ggttcaggta
     5701 cgctttgtat tgaagcagcc ttaataggcc ataatatcgc accaggattc aatcgtgagt
     5761 ttgcttgcga atcttgggat tggttcagtc aagaaatcat ggaaaatgtt cgatcagcag
     5821 ccgaagaaaa agcagattat gatattgaat tagatattac tggttcagat atcaatggac
     5881 ggatgattga aattgccaaa gccaatgcag aagaaatcgg attaggctct tcgattacct
     5941 ttaagcaaca agcagtcaaa gacttcaaaa cggataaaga atacggggtc atcgtggcta
     6001 atccgcctta cggagaacgt ctaggagaag aagaaactgt tcggaaatta tataaagaaa
     6061 tgggcgatgt attccgtcca ttaaaaacat ggagtaaata tatcttgact agcgacttag
     6121 cgtttgagga attttacgga caaaaagcaa ccaaaaaaag aaaattgtac aatggtgcat
     6181 tgcgtacgga tctattccag tattggggaa ctcgtccgcc tagaaaaaaa gagaaagacg
     6241 aataatctta ctaattgatt cattcaattg gatgtttgga ggaataggga atgaaagaaa
     6301 aagagttttt acaagaagta aaagaaatca atcttctaca acaagcttta ggaatcctcg
     6361 attgggatac acaaacaggt atgcctgaaa aagctagtgc ataccgaagt gaagtagaca
     6421 gctatcttta cagcatttat ttttcaaaaa aaatcggtcc aaaaatcaaa gaagccattc
     6481 gctatttttc tgaacatccg gaagaattgt cggaagttgg aactgccgtt tttgccaaag
     6541 taaaagaaga gtatgatttg gaacatcgcg taccggaaga tcttatgaga aaatatacag
     6601 ctgccgtttc ttcagcacac ggacaatggc aaaaagctag agaaacacaa caattttctg
     6661 attttcaaca cgcattgaat caaaatattg aattaacaaa acagttgata ccttactgga
     6721 gaaaagatga aaagacgaat tatgatgttt tgctgaatca atacgaaccc gggatgactg
     6781 tcgaaatcct tgataaggta tttgatcagc ttaaagaagg aattctttcg atccgccgta
     6841 cgctccaaga aaaagggact gaacctgaaa cagacttttt aaatcggaga atgacgaaaa
     6901 aacaacagaa gagatttgta gtgaaagtaa tcgaacaact tggttatgat tttagtcgtg
     6961 gtcgtttaga tgacactgtc cacccattta tgactggaat taatccgaat gatgcacgaa
     7021 tcacgacacg atggaacgaa acagatttca aaatggctgt ttttggagtg atccacgagg
     7081 ctggtcatgg tatgtatgaa caagacatcg atgataaata tgcgtataca ccggtttatg
     7141 aaggtacatc aatgggtatc catgaatcac aatcgttgtt taacgagatc attattggaa
     7201 gtagtcgcag cttctggaaa aagcaatatc ctttctttca agaatgtgca gaaggaacat
     7261 tcgatgatat tccttttgac aaattttatc gttcattgaa ccatacaaaa tcaagtttgg
     7321 ttcgtatcga ggcagacagt ttgacttacc ctctccatat catcattcgt tatgaaatcg
     7381 aaaaaatgat tttcaatgaa ggggtagatg ttgctgaact acctgaaata tggaatcaaa
     7441 aatatgaaga gtatctggga atacgaccaa caaatgatgt ggaaggcatc ctacaagacg
     7501 ttcattggtc aggtgcaagc tttggttatt ttccctctta tgcattaggg tacatgtatg
     7561 cggcacaatt acttcattct ttgcagcaag aaatagatgt ggaaaatgtt ctagcgtcag
     7621 acgattattc tccaatcaaa aactggatgt cagagcatat ccatcaatat ggtgcatcta
     7681 gaaaaccaaa tcaattaatc atggatgcaa cacatgaacc gcttaatccg caatacctga
     7741 tcgactatat gaaagacctg tattttaatg tatatcaggt aaaataaaat acaatttaca
     7801 aagatagctg aaattcatct aatctataga tgttttcagc tatttactta agaaagaagt
     7861 ggaacgaatg gataaattac aagtgatcgc tgattatagt atggaccaat tagcgcatga
     7921 tcaaacagga catgggagcg atcataccaa aagagttgtc aagttagctg aaaaaatttt
     7981 ggagacggaa ccgcaggcag accgctttgt gacgcttgca gcagcttacc tacatgatac
     8041 gattgatgat aaagtcgtta aaaatgagaa cgaggcaaag caacagcttc gtgtattcct
     8101 tcgctctttg ccaatagtag aggaacagat ctcgatgatt ttttccatta tcgaaaatat
     8161 gtccttttct aaaaatctct ctgaagcggc cgaactatct ctggaaggta agatcgttca
     8221 agacgctgat cgtatcgaag cattaggagc aatcggcatt ttgcgtactg cttatttcgg
     8281 cggtggacat gggcacccga tttttgatcc tgaactgtat ccgcaaacat tcaaggacaa
     8341 agaaaattac cgaaaaggca cgacagtgat caatcatttt tatgaaaaac tgttcttgct
     8401 gtctgataaa atgaatactg attacggata tgaagaagcc aaacgaagag aaaccttcat
     8461 gcgtgaattc ttagaagaat tttttctgga atggtctatt gacgatgaaa attttactcc
     8521 agacaattgg atcaaaaata acaaaaaatg atggaggttg tgacagaagt ggtcagctcc
     8581 aagatatttc ggatcatttt ccgtaaaagt tacttctgga acactgttta ccaagaggtc
     8641 gtgagcctgc cgttcagctc cgagtcacaa ggaacaattt caaaaaatag cttctcgtgt
     8701 ttgatgaata ttgtaacttg tgattcggaa ttggcaggag aacaccgttt att
//
LOCUS       DAZKLM010000104         8624 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3764
            SAMEA115706913-rid22582563.denovo.108, whole genome shotgun
            sequence.
ACCESSION   DAZKLM010000104 DAZKLM010000000
VERSION     DAZKLM010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706913
            Sequence Read Archive: ERR13196647
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8624)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8624)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287823.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287823.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,004
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,849
            CDSs (with protein)               :: 2,849
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 41 of 96
            Pseudo Genes (incomplete)         :: 52 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 15 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8624
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706913-rid22582563.denovo.108"
                     /isolate="VRE3764"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(37..750)
                     /locus_tag="ACG842_002384"
     CDS             complement(37..750)
                     /locus_tag="ACG842_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0248888.1"
                     /translation="MKKLGFILLLVSLFLVGCTSPSKENSDPSATTDKSSIRQTSSAE
                     KSSTQKSASTETNTSSTSSKVSEPNTSSTSSSTVETNISSSTIDTTSSQSSQSIDPLA
                     GYSDLQIEYARVWLAIRGNTFRDTSGDFELHVIHYPAGTPIAPYDAGSAVYPKDVVML
                     TGKYSYQGVLVYSSNHDGTITSYPVPSHWQMPADQTSYPAFIRETTQEIIDNASVVAI
                     PTGNPNDVKQLIDVTVIDN"
     gene            complement(923..1530)
                     /locus_tag="ACG842_002385"
                     /pseudo
     CDS             complement(923..1530)
                     /locus_tag="ACG842_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313657.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0019172 - glyoxalase III activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase family
                     protein"
     gene            2014..2241
                     /locus_tag="ACG842_002386"
     CDS             2014..2241
                     /locus_tag="ACG842_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HFQ0248889.1"
                     /translation="MKIAFHIFADFDLMPKVSFAITVYREAVKAPCSSDDNKQAFLEL
                     GFLDSKYAAYFNELVLERDFKKVENRHDFST"
     gene            2257..3422
                     /gene="hemW"
                     /locus_tag="ACG842_002387"
                     /pseudo
     CDS             2257..3422
                     /gene="hemW"
                     /locus_tag="ACG842_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299641.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
     gene            3656..4699
                     /gene="hrcA"
                     /locus_tag="ACG842_002388"
     CDS             3656..4699
                     /gene="hrcA"
                     /locus_tag="ACG842_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HFQ0248890.1"
                     /translation="MLTKRQNDILRLIIQHYTSSGVPVGSKTLMSEGVKASSATIRND
                     MKALEDEGLLLKTHSSSGRIPSALGYRYYVDHLLKPARVAEDDLLLIRQSLGREFHEI
                     NEIIKQSAEILSELTSYTTFSLGPEIKDRRLTGFRIVPLNNRQIIAIVVTDMGNVESQ
                     VFTLPEYMGSQELEKMVQIVNDRLVGDPLMTVYHKLRTEIPMILHKYFQTTEGMSQLF
                     DTVLGHVFEDKVYVSGQMNLLDYEPRQDLDQFKSMFSFMKDSDGLTQMITPMDSNIHI
                     KIGAELGNDLLQNMSMIQASYEIDGHGRGTIALLGPTSMPYSRMFGLVDVYRRELAAK
                     LADYYRSLDLSNS"
     gene            4793..5356
                     /gene="grpE"
                     /locus_tag="ACG842_002389"
     CDS             4793..5356
                     /gene="grpE"
                     /locus_tag="ACG842_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HFQ0248891.1"
                     /translation="MKENQEELDKEMTDAQPEPEIDVEAAEDSGISEAEAEEFETAKL
                     KAELEEMEDKYLRARAEIANMANRGKNEREQLQKYRSQDLAKKLLPSIDNLERALATE
                     VSDDQGAGLKKGVEMVLESLRNALEEEGIEKIPAKGEAFDPNLHQAVQTVPATEDTPA
                     DTIVEVLQEGYKLHDRVLRPTMVIVAQ"
     gene            5407..7236
                     /gene="dnaK"
                     /locus_tag="ACG842_002390"
     CDS             5407..7236
                     /gene="dnaK"
                     /locus_tag="ACG842_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016635026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HFQ0248892.1"
                     /translation="MSKIIGIDLGTTNSAVAVLEGNEAKIIANPEGNRTTPSVVSFKN
                     GEIQVGEVAKRQAVTNPNTISSIKRHIGEAGYKVDVEGKSYTPQEISAMILQYIKGFA
                     EDYLGEKVEKAVITVPAYFNDAQRQATKDAGKIAGLEVERIVNEPTAAALAYGLDKTD
                     RDEKILVFDLGGGTFDVSILELGDGVFDVLSTAGDNHLGGDDFDNKIIDYMVAEFKKE
                     NSIDLSQDKMALQRLKDAAEKAKKDLSGVSSTQISLPFITAGESGPLHLEMTLTRAKF
                     DELTADLVERTKVPVRQALKDAGLSQSEIDEVILVGGSTRIPAVVEAVRKETGKEPNK
                     SVNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTS
                     KSQVFSTAADNQPAVDIHVLQGERPMAADNKTLGRFQLTDIPAAPRGIPQIEVTFDID
                     KNGIVNVSAKDLGTQKEQKITIKSSSGLTDEEIERMVKDAEANAEADKARKEEVDLRN
                     DVDALLFSVDKTLKELEGKVDEEEVKKAETARDELKAAVEANNIEEMKTKRDALNEIV
                     QNLTVKLYEQAAQQQAQENPEAAQGGADDVVDADFEEVDGDDK"
     gene            7387..8553
                     /gene="dnaJ"
                     /locus_tag="ACG842_002391"
     CDS             7387..8553
                     /gene="dnaJ"
                     /locus_tag="ACG842_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HFQ0248893.1"
                     /translation="MATKRDYYEVLGLSKGASEDEIKKAYRKLSKKYHPDINKEPDAE
                     EKFKEVSEAYEILSDPQKRAAYDQYGHAGTDPNYGAGGAGGGFGGFGGFSGGGFGGFE
                     DIFDSFFGGGGRSVDPNAPRQGADLQYTVDLSFEEAIFGVEKEIKYNREEICHTCGGN
                     GAKPGTQPTTCHKCHGAGTINVERQTPLGRVMSRQTCDVCHGTGKEIKEPCPTCHGSG
                     HEKKAHSVKVNVPAGVEEGQQMRLAGQGEAGENGGPFGDLYVVFRVEESDIFDREGSE
                     IYYELPLSFVQAALGDEVQVPTVHGDVKLKIPAGTQTGTNFRLRGKGAPKLRGGATGD
                     QHVKVKLITPKNLNEQQREALRAFAEAGGIKVEEQQEDGFFDKFKDAFGGKKRK"
BASE COUNT         2914 a         1490 c         1839 g         2381 t
ORIGIN      
        1 gctgtcagct tctttttgta taacatatta gaaagtctaa ttatctataa ctgtcacatc
       61 gattaactgt ttcacgtcat tgggatttcc tgtaggaata gctactacag atgcattatc
      121 aatgatttct tgagttgttt ctctaatgaa cgctggataa gatgtttgat ctgcaggcat
      181 ttgccaatgt gaaggaactg gataactagt gattgtcccg tcatggttac ttgaatacac
      241 taaaactccc tggtaactgt atttcccagt gagcataacc acatctttcg gatagactgc
      301 actccctgca tcataagggg cgataggtgt tcctgcagga taatgaataa catgtaattc
      361 gaaatcaccc gatgtatctc taaaagtatt acctctaata gctagccaaa cacgtgcgta
      421 ctcaatttgt agatcagaat atcctgctaa cggatctatt gactgagaag actgtgagga
      481 ggttgtgtct attgttgaag aagaaatatt tgtctcaact gtactactag atgtagaaga
      541 agtattgggc tctgagactt tactgcttgt tgaagatgta ttcgtttcag tagaagctga
      601 tttctgagta cttgattttt cagctgaact ggtttgtctg attgaacttt tgtctgtggt
      661 cgcagatgga tcactatttt ctttactagg agacgtacat cctacaagga ataaagagac
      721 caacaataaa ataaaaccta actttttcat ataaaatacc tcattatttt tttaatacca
      781 gtatagcata atcctgaatt tttgaatccg taaaaccttg tctatgaata taaatagagt
      841 gtatcttctt tgataaatgt cagaaaaagt gtgacaaata atcatcagta aaattacttg
      901 tcacactatt gataaggagt atttaattga agagaatgat taggagaaag gatctccgta
      961 ctttttatag taatcataga atccaagttt gtatagacga atcgtttttt ctcttatttc
     1021 ttctgtttca aaatggactg cttgaagaat caagtctgtg aattctatag gagctgttcc
     1081 attcgcagta atcaaattac gatctttcac agcttgttgt tctataaaat cttctttatt
     1141 ctgataattt tccgattcta accaaggaaa ttgcgcattt ccggtgtgtt tgtaaccttg
     1201 caataatccg ctctgagcta aataatcaac tgccccacaa atagcagcta ctggtacgtc
     1261 tgtttttaac aatttatcga ttatttgata taaatgatga ttttttatgt cccatgaatt
     1321 gccaccgatc ataataaaaa ggtcaacttt ttcaggtatt tgttcgatta agtaatcgat
     1381 ttttgttttt aatcctccaa tggaagtgac ctctgattgg atagaagcag ttttgttttg
     1441 ccatttctca ttttggttta gtgtgctagt aatataagca ccttcccaat cggcatattc
     1501 ctctaataga aacgtaatcg cagttttcat tttcaagctc tcctttacta tctataacaa
     1561 ggacaacagt tttaacctac agacattcat taaaattggg tagcgacagc gaaaacaaca
     1621 attcttaagt tttagacaga gtatacagat agtcaactta attaaatacg aactaaataa
     1681 aaatgacagg taatcatttt tgtttaccag cattataaca ttttgcatac atggatgaca
     1741 aaagaaccaa ttcccacttg cttgagtaca atattaaaag aaactggaag gcaaaaagta
     1801 attatagaaa cgaaaaataa atcatatcgg gcactcagac atgaaaacaa ccctagagat
     1861 atataactag gtaaccagtt taatgaaaga aaaaatcatc caagaagtcg attcttggat
     1921 tttttagtgg atttgttcgc caaatacgtt tagtatgcgt atatgagagg ttgtaagcaa
     1981 agcgttgtgg tctgagcact taaggaggaa tctgtgaaaa tagcttttca tatttttgca
     2041 gattttgact taatgccgaa ggtcagcttt gcgattaccg tttatcgaga ggctgtaaaa
     2101 gcaccgtgta gctccgatga caataagcaa gcatttttag agctaggttt ccttgattct
     2161 aaatatgcgg cttatttcaa tgagttggtg cttgaacgcg attttaagaa ggtcgaaaac
     2221 aggcacgact tttcgacgta aggagaaaga atttcaatga tagaaacaat aaatcccgca
     2281 gaaaacaagg tttctgcgta cattcatatc ccattttgcg agcatatctg ctattactgt
     2341 gattttaata aagttttttt agaagggcag ccagtagatg agtatgtgga aatgctgctg
     2401 aatgaaatga aaataatgct agaaagtcat ccgacagcga atttagaaac actttacgta
     2461 gggggcggga caccaacttc tttatctgct aaacaacttg atcgactgtt atcaggtgct
     2521 agagagatcc tgccttttca aaatggtaat gagtttactg tagaagcgaa tccaggtgat
     2581 ttgacccgtg aaaaacttca agtaatgaaa aactatggcg tcaatcgttt atccatggga
     2641 gtgcagacat ttgataatcg cttattgaaa aaaattggac gtaaacacac cgcagaagat
     2701 gtgtatcaaa ctatgcaatt tttagaagca gaaaatttta ccaatgtaag catcgatttg
     2761 atttatgcat taccgggaca aacactagaa ggatatctgg atactttgga aaaagctttg
     2821 gctttggatc tgcctcacta ttctttgtac tcattgatcc tcgaaaacaa aacaatgttc
     2881 atgaactggg tacgacaagg aagactcgaa ctgcctgatc aggaaacgga aaccagaatg
     2941 tttgaagaga cgattgaagc aatggaaaag catggacgtc accaatatga aataagtaac
     3001 tttgggctct ccggacatga atctcaacat aacctcatgt actggaacaa tgaccactat
     3061 tttgggtttg gagctggtgc tagcggttat cttggagata ttcgttaccg caacaaagga
     3121 ccaatccaac actatttacg cccattacga gcaaatgaac gcccggtatt ggaagaagaa
     3181 aaattgacac gaaaaaatca aatagaagaa gaaatgttct taggactaag aaaaaaacag
     3241 ggattactaa aaaacatttc tatgatcgct atcaaaaaac aattgaatcg gtgtacggtg
     3301 atgtgctaag ggaattagag caagaagggt tacttgttaa tgaagaagac cgtgtttatt
     3361 tgacaccaag agggaccttt ttggggaaca atgtgtttga gcgctttttg tttgaaaaat
     3421 agtttaaaga atatttgttt tttatctgaa tttaaataaa aaaagttcaa aaatacaata
     3481 aaggactatt gaaacgttga tataactgga tttacatcaa ttatttagaa ttagcactct
     3541 tgtgtttaga gtgctaaaaa atggagtctt tctcttgaca ggagaagggc ttcttgctat
     3601 atttataagt ggattagcac ttagaatata tgagtgctaa tgagaggtga ggaagatgtt
     3661 aacaaagcga caaaacgaca ttttacgtct gatcatccaa cactatacga gtagtggcgt
     3721 gcccgttggt tccaaaacat tgatgtctga gggagtcaag gccagctcag caacgattcg
     3781 caatgatatg aaagcattag aagatgaagg attgctgttg aagactcact cttcttctgg
     3841 gcggattcca tctgctttag gctatcgtta ttacgtggat catttgttaa aacctgctcg
     3901 cgtagcagaa gatgatttgc tgctgattcg tcagtctcta ggcagagaat ttcatgaaat
     3961 caatgagatt attaagcaat ctgctgagat attatctgag ctgacaagct atacgacttt
     4021 ttcattggga cctgaaataa aagaccgccg actgactggc tttcgtatcg tacccttgaa
     4081 taatcgacag attattgcta tcgtcgtaac agatatgggg aatgtagaaa gccaagtgtt
     4141 cactcttccc gaatatatgg gaagtcagga actagaaaaa atggtacaga ttgtaaacga
     4201 ccgtctagtc ggagatcctc tgatgacggt gtaccataaa cttcgtacgg agattccgat
     4261 gattcttcat aaatatttcc aaacgacaga agggatgtct cagttatttg acaccgttct
     4321 tggtcatgtt tttgaagata aggtttatgt cagtggacaa atgaatctct tggattatga
     4381 gccccgtcaa gatcttgatc agttcaaatc gatgttttct tttatgaaag actcagacgg
     4441 acttacacaa atgatcactc cgatggatag caatattcat atcaagattg gtgctgaatt
     4501 aggcaatgat ttattgcaga atatgagtat gattcaagca agttacgaga tagatggtca
     4561 tggaagaggt acaatagcct tgttagggcc tactagtatg ccttattctc ggatgttcgg
     4621 tctagtcgat gtttatcgaa gagaactagc agctaaactt gctgattact accgttcgct
     4681 tgacttgtca aactcgtaat gtgaataacg aagctatttg cagctgatcg ttctctcttt
     4741 cttagtaaga gcaatcaaac agctgactga ataatgaaag gaagactgta tcgtgaaaga
     4801 aaatcaagaa gaactagata aagaaatgac agatgctcag ccagaaccgg aaatcgatgt
     4861 cgaagctgca gaagattcgg gtatttcaga agctgaagcg gaagaattcg aaacagcgaa
     4921 attaaaagct gagttagaag aaatggaaga taaatacttg cgggctcgtg ctgaaatcgc
     4981 aaatatggca aatcgaggaa aaaatgagcg tgagcaatta caaaaatacc gttcacaaga
     5041 tttagccaaa aaattacttc catcaattga caacctggaa cgtgcattag ccacagaagt
     5101 gtcagatgat caaggggcag gtttgaaaaa aggcgtggaa atggtcttgg aaagtttgcg
     5161 taacgcactg gaagaagaag gaattgaaaa aatccctgct aaaggagaag cctttgatcc
     5221 taatcttcat caagcagtac aaactgttcc agcgactgaa gatacaccag ccgatacgat
     5281 tgtagaagta ctacaagaag gctacaaatt acatgatcgc gtgttaagac caaccatggt
     5341 cattgttgca caataattgt gcggatataa aacttacact ttcaattaaa gaaggagacg
     5401 ttaattatga gtaaaattat cggtattgac ttaggtacga ctaactctgc agtagcagta
     5461 ttggaaggaa acgaagcaaa aatcatcgct aacccagaag gaaaccgtac aacaccttct
     5521 gttgtatcat tcaaaaatgg agaaatccaa gtaggggaag ttgcaaaacg tcaagctgtg
     5581 acaaacccaa atacgatttc atcaatcaaa cgccatatcg gtgaagcagg ttacaaagta
     5641 gatgtagaag gtaaatcata tacaccacaa gaaatctcag caatgatcct tcaatatatc
     5701 aaaggattcg cagaagacta tttaggtgaa aaagtcgaaa aagcagttat cactgtgcca
     5761 gcttacttta atgatgcaca acgtcaagca acaaaagatg ctggtaagat cgctggtcta
     5821 gaagtagaac gtatcgtcaa tgaaccaact gcagctgctc ttgcctatgg tttagataaa
     5881 acagatcgcg acgaaaaaat cttagtattc gaccttggtg gcggtacttt tgacgtatct
     5941 atccttgaac taggagacgg tgtctttgat gtattgtcaa cagctggcga caaccatcta
     6001 ggtggggacg actttgataa caaaatcatc gactacatgg tagcagaatt caaaaaagaa
     6061 aacagcattg acttgtctca agacaaaatg gctttacaac gcttgaaaga cgctgctgaa
     6121 aaagcgaaaa aagatttatc tggcgtatca agcacacaaa tcagcttgcc atttatcaca
     6181 gcaggagaat ctggaccatt gcatttggaa atgacattga cacgtgcaaa atttgatgaa
     6241 ttaacagctg acttagtaga acgtacaaaa gtaccagtac gtcaagcgct aaaagatgca
     6301 ggtttatcac aatcagaaat cgacgaagtt attttagtcg gtggatctac tcgtatccct
     6361 gcagttgtag aagcagtacg taaagaaaca ggaaaagaac caaacaaatc agttaaccct
     6421 gatgaagtag ttgctatggg tgctgctatc caaggtggcg tgatcactgg tgatgtgaaa
     6481 gatgttgttt tacttgacgt tacaccattg tcattaggta tcgaaacaat gggtggagtg
     6541 ttcactaaat tgattgaccg caatacaacg attccaacaa gtaaatcaca agtattctca
     6601 actgcggcag ataatcaacc agcagtagac atccatgtat tacaaggtga acgcccaatg
     6661 gctgctgaca acaaaactct aggaagattc caattgacag atattccagc agctcctcgt
     6721 ggtattccac aaatcgaagt aacattcgat attgataaaa acgggattgt aaatgtatct
     6781 gcgaaagatc taggtacaca aaaagaacaa aaaatcacga tcaaatcttc ttcaggtcta
     6841 acagacgaag aaatcgaacg tatggtaaaa gatgcagaag caaatgcaga agcagataaa
     6901 gctcgtaaag aggaagttga cctacgtaat gatgtagatg cattgctatt ctctgtggac
     6961 aaaacattga aagaattaga aggcaaagtg gacgaagaag aagtgaaaaa agcagaaaca
     7021 gctcgcgatg aattgaaagc cgctgttgaa gcaaacaaca tcgaagaaat gaaaactaaa
     7081 cgcgatgcat tgaacgaaat cgttcaaaac ttgactgtta aactatacga acaagcggca
     7141 caacaacaag ctcaagaaaa cccagaagca gctcaaggcg gagcagatga tgtcgttgat
     7201 gctgattttg aagaagtcga tggcgacgat aaataatcag ttcactgaat ttcgagagaa
     7261 ggccaaagtt gctactttgg ctttttcttt tgaaaaatag tttgaagtat cggtttttgt
     7321 ttcaaacaaa atatgatatg ataacaagcg acgtgaaaat gatgagaacc aataggagga
     7381 aagcctatgg caacaaaacg tgattattat gaagtcttag ggctctcaaa aggagcatca
     7441 gaagatgaaa taaagaaagc ttatcgtaag ctttcgaaaa aataccatcc tgacatcaat
     7501 aaagaacctg atgcggaaga aaaattcaaa gaagtatcag aagcatatga gattttaagt
     7561 gacccgcaaa aacgcgctgc atatgaccaa tacgggcatg caggcacaga tccaaattac
     7621 ggtgctggtg gagctggtgg cggatttggt ggattcggcg gcttctctgg tggcggcttc
     7681 ggtggttttg aagatatctt cgactctttc tttggaggag gcggtcggtc agtcgatcca
     7741 aatgcgcctc gtcaaggtgc tgatttgcaa tatacggtcg acttgtcttt tgaagaagcg
     7801 atttttggtg tagaaaaaga aatcaagtat aatcgggaag aaatctgtca tacttgtggt
     7861 gggaacggtg caaaaccagg cacacaacca actacttgtc ataaatgtca tggtgcaggt
     7921 acgattaatg tagaacgcca aacaccactt ggacgagtga tgagccgtca aacttgtgat
     7981 gtttgtcatg ggacagggaa agaaatcaaa gaaccttgtc caacctgtca tggctcagga
     8041 catgagaaga aagcacattc agttaaagtg aatgtaccag ctggtgtaga agaaggacaa
     8101 caaatgcgtt tagctggtca aggagaagct ggagaaaacg gcggaccatt tggtgatttg
     8161 tatgtggtct tccgtgtaga agaaagtgat atctttgatc gtgaaggttc tgaaatttac
     8221 tatgaattgc ctttaagttt tgtacaagca gctttggggg atgaagtcca agtaccaact
     8281 gtccatggag atgtgaaatt gaagatccct gctggaacac agacaggtac gaacttccgt
     8341 ttacgtggaa aaggcgctcc gaagttacgt ggtggtgcaa caggcgacca acatgtcaaa
     8401 gtaaaactga tcacaccgaa aaatctaaat gaacaacaaa gagaagcatt acgtgcattc
     8461 gctgaagctg gcggcatcaa agttgaagaa cagcaagaag acggtttctt cgataagttt
     8521 aaagatgctt ttggcgggaa aaagcggaag tagtttttct agtaaaaacg ctaagtcatt
     8581 aataattaaa aaccagacga acaatcatat gttcgcctgg tttt
//
LOCUS       DAZKLM010000105         8494 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3764
            SAMEA115706913-rid22582563.denovo.109, whole genome shotgun
            sequence.
ACCESSION   DAZKLM010000105 DAZKLM010000000
VERSION     DAZKLM010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706913
            Sequence Read Archive: ERR13196647
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8494)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8494)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287823.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287823.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,004
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,849
            CDSs (with protein)               :: 2,849
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 41 of 96
            Pseudo Genes (incomplete)         :: 52 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 15 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8494
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706913-rid22582563.denovo.109"
                     /isolate="VRE3764"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..149)
                     /locus_tag="ACG842_002392"
     CDS             complement(<1..149)
                     /locus_tag="ACG842_002392"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0248894.1"
                     /translation="MENHVLETGMQKITTHASITAKPFFEKRGYKVINEQTVELRGQL
                     FTNFL"
     gene            complement(593..1714)
                     /locus_tag="ACG842_002393"
     CDS             complement(593..1714)
                     /locus_tag="ACG842_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HFQ0248895.1"
                     /translation="MDKYNVKTSFQKWFSAINFSGLSENSQSLISDFDFYSKKLDFQT
                     TLKVLLHAVYEELPSYREIDRAFLDQRLCKELGIDSLCYSSLSRRAPEIKQEVLMEIF
                     TQLVARISAQQPSSKTTSLQLIDSTTIPLNKAWFPWAKFRKTKSGIKLHLNLCYLDKT
                     NQYPESFTMTNASEHDRNHLEVLVDKTQATYVMDRGYFDYKLLDKLNRDGYFFVTRTK
                     SNTKITILDQIEVADTTTRDGTIISDQQVILVGGVNHVTERFRLVTVLTKGQKILRMV
                     TNLFDVSPNEVADMYQARWQIELLFKHLKQNLTIKRLYSHSEQGAINQVILTLIATLL
                     TYVIKIELNTTATLFQLKRTFHYLMFEPAEEWFKRYKPG"
     gene            2042..3451
                     /gene="istA"
                     /locus_tag="ACG842_002394"
     CDS             2042..3451
                     /gene="istA"
                     /locus_tag="ACG842_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HFQ0248896.1"
                     /translation="MDDIKTIKHLRNNRDQSIDQIRKAMNINWRTAKKYADSDQLFIT
                     DPTRKNKSMMDDGWGEIIGSWLEEDLRLRRKLRRTAKAIHEQLKPLGFPGSYRTVCVY
                     ISQWKKQRYDETNYQAVDRLDHPIGEAQVDFGTMEVMHEDHMKDIQLLIMSFPYSNAT
                     SASAMPAQNQECFLEALKQLFNQCGGVPKTIRIDNLTPAVKKTKSKFEEAQLTDEFLR
                     FTIHYGCDVQVCNPRSGHEKGHVENKVGYVRYNFFSTSPIMDSFESLNKQLEEQLKAD
                     RKRTHYSKHQLIEDLWKKENKALHSLPQEDYPIFKEVEAKLNRYHEMKLDQTVIHIPK
                     AYGYSQLTVVLTWNKFKVLTPHGEILMNEYRPYMNKKRLIPWSDIMKQWSNKLNVVSY
                     SRYWKYLPARLQNYLAIDNNHLKYRRIKQLLSLLATYDIQQINEQFYELVVQEDAPSD
                     FDVNWSNYDALTKTGGVQK"
     gene            3448..4176
                     /gene="istB"
                     /locus_tag="ACG842_002395"
     CDS             3448..4176
                     /gene="istB"
                     /locus_tag="ACG842_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element helper ATPase IstB"
                     /protein_id="HFQ0248897.1"
                     /translation="MIGQTLEDYCKKLRLSHIITNIPRASFEAPEQFITDLFRLELET
                     RQQIKLERMEKLAKFPVPKSLKNYEWHEDIFLPSHLDKETLVSLDFLKRQENIVCVGS
                     PGTGKTHLAIALGRKACSEGVSTKFFRVNNLVEQLENALKKQQLASFKKRFETTELII
                     LDEMGYLPLTKEGAELLFQLISDWYEQKSIIITSNLEFSQWNRVFMDSRLTAALVDRL
                     IHHAHIISFKGESFRLKNSLSKSY"
     gene            complement(4304..5215)
                     /locus_tag="ACG842_002396"
     CDS             complement(4304..5215)
                     /locus_tag="ACG842_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFQ0248898.1"
                     /translation="MKIKIERSNKEKIPKKKKERVVSVGKHRKMVLALWLLLSCSLAF
                     GIYKNFTAIDQHTTHEKVVVKEKIVNTSGVENFTVDFVKEYFSWKNNKEVIEKRMTNL
                     EQYLTEEGLALSQDMVRADIPTSSEVVSVKILDVEKHSEEFVVSFVVDQKITEGKKTQ
                     SISSAYRLMIFEDEKGNHIVTSLPTMIGKPEKAKHKVRQVKSDSKIDSKTTEEITEFL
                     ETFFRLYPAASEKELEHYVEKDAMQPINTNLKFVELVNPIYKQKGKAIQVIVGVEYLD
                     ELSKTTSVFQYRLKLYKNENWTISNCE"
     gene            complement(5232..6239)
                     /locus_tag="ACG842_002397"
     CDS             complement(5232..6239)
                     /locus_tag="ACG842_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HFQ0248899.1"
                     /translation="MNLKKLSIISLTFLTISFVGILMCVGLLFGEDGEGSGSSGTSPG
                     GSSVSEVVLKHRSMVEKYAKESDITEYVSILLAIIQVESGGTMEDVMQSSESAGLSPN
                     SLSTEASIKQVCLYFSSLVKCSKELGCDQDSIIQAYNYGDGYLDYVAKNGKKHSFALA
                     ESFSKEKSGGQKVDYPNPIAIKENSGWRYNYGNMFYCLLVKQYLTTTQFDDKTVQGIF
                     DEAFKYEGTAYVFGGSSPGTGFDCSGLTQWCYAKVGLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLIFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTDSYWQQHIIGAGRYK
                     Q"
     gene            complement(6236..8365)
                     /locus_tag="ACG842_002398"
     CDS             complement(6236..8365)
                     /locus_tag="ACG842_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFQ0248900.1"
                     /translation="MKKKLFRIVGIVFISIVLLTVLLSLVGTVAEATGLVDDTVEAGN
                     LYSKYSLNNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAKSIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRTVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALSLGTKIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEIGEGRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKRAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMSMFNLQSNDSQSMGRRVMRKPQMLMNRKLR
                     QLNRNVGRTFAFGGGAAVGGKLATSSTKGKGSSKINRVSNRKNHSARSINELNSDLKK
                     QEQPVGTKKESRINLVGRKVGKVLDTKEIVKDKAKQAKNQVTDAPTNLKYSLHKGMEK
                     TKKVPEDFKRGPFEEKANRGELREKQRQRRDAKMDEKRKVLNEAWNRHEKGRENVPIK
                     KEIKPQKNELPENRSLKPRVQVHSNPEIKRKLSSQEVIPNGSTQPLSKNNSFQQVKQR
                     FTFPKRTNQQVQRKKNTRPKPKPGEKR"
     gene            complement(8370..8480)
                     /locus_tag="ACG842_002399"
     CDS             complement(8370..8480)
                     /locus_tag="ACG842_002399"
                     /inference="COORDINATES: protein motif:HMM:NF024254.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0248901.1"
                     /translation="MMQDLYGRVGVVQVHPVFEELFHAFDTRPPVQERME"
BASE COUNT         2600 a         1726 c         1485 g         2683 t
ORIGIN      
        1 attaaaaagt tagtaaataa ttgccctcta agttccacgg tctgttcatt aataacttta
       61 tatcctcgtt tttcaaaaaa tggctttgcg gtaatggaag cgtgggtagt gattttttgc
      121 ataccagttt ctaaaacatg gttttccaat tcatctgcaa ttttagtcgc aatacctttt
      181 ccttgatggc tatgtttttg tcacaacttc agtattcctg taacaatacg gatttcagta
      241 tacaacaaca gcttttgaaa agatacttgc aaaaggatta agatatctat gacctgctat
      301 gctcactgat gttgaaaaga atgttttatt aggattgagg taagcaagat agtgaatatg
      361 tccggaaagc ctgtaaaata aggcttttcg gacatatgtg aagttataaa aagatagcga
      421 aaaatctatt tagtatcact aatgttgcga acatatcgtc agaatagtct gacatttatt
      481 tcttgctaaa aatttcaaaa aagtcaagtg ctgacgcagc ggatgactat tttccagaaa
      541 attttagtat gtattcaaaa atcaaacgtg acaacagcta ttaactacat cctcatccag
      601 gtttgtaacg tttgaaccac tcttctgcgg gctcaaacat taagtaatgg aaggtacgtt
      661 tcaattgaaa cagcgtcgct gtcgtattca actcaatttt tatcacataa gtgagaagcg
      721 ttgcgattaa ggtgaggatg acttgattta tcgccccttg ttcactatgt gaatatagtc
      781 gtttaatggt cagattttgt tttaagtgtt tgaacaacaa ttcaatctgc caacgggctt
      841 gatacatgtc ggccacttcg tttggcgata catcaaagag attggtcacc attcgcagga
      901 ttttttgtcc cttggtaaga accgttacca acctaaaacg ctctgtcaca tggttgacac
      961 cgccgacaag aatcacttgt tggtcactga ttatcgtccc atcacgggtt gttgtatctg
     1021 ctacttcaat ttgatccaag atggtaattt ttgtattaga ttttgtccgt gtaacgaaga
     1081 aatagccgtc acgatttagt ttgtctaaca acttgtaatc aaagtagccg cgatccatca
     1141 catacgttgc ttgcgtttta tcaacaagaa cttcaaggtg attacggtca tgctcactcg
     1201 catttgtcat cgtaaaactt tcaggatact gattggtttt atcaagataa cagagattca
     1261 aatgtagctt gatgccagac tttgttttcc gaaacttggc ccatggaaac catgctttgt
     1321 tcaacggaat agtcgttgaa tcaatcaatt gtagactggt tgtttttgag cttggctgct
     1381 gagcagaaat tctggcgact aattgcgtaa aaatttccat caatacttct tgcttgattt
     1441 ctggcgctct acgagataac gagctgtaac acagggaatc aatgccaagt tccttgcaga
     1501 ggcgctgatc taaaaaggcg cgatcaatct cgcgatagct tggcaattct tcataaaccg
     1561 catgaagtaa cactttaagc gtcgtttgga agtctagttt tttactgtag aaatcaaagt
     1621 cgctaattaa gctttgagag ttttctgata aaccagaaaa atttattgca gaaaaccatt
     1681 tttgaaatga ggtttttacg ttatacttat ccatgcgtga gtccttttta ttggtcttgg
     1741 ataagtcatc cgagaatagt ataaaggact tttttgcgtt tggatagctt tgtattaggc
     1801 ttttgatggg ttaaaaagtc tttgcaacac tagtaattta gtatttatgt ataaattgaa
     1861 agatatttta ccaccactta gtcttcttaa aagtgatcca acattttgtt tgtggcagaa
     1921 tttaactaca cactaatgca gccgaaagta cacaactatg cagcagtttg actgcgaact
     1981 ggtttccttc catcataagt ttgaatgact tagtgatgga ggtacagaaa ggttgttaac
     2041 tttggatgat attaaaacaa taaaacattt gagaaataat cgtgatcagt caattgatca
     2101 gataagaaaa gcaatgaata tcaattggcg aacagcgaaa aaatacgccg atagtgatca
     2161 actattcatt acagatccca ctcgaaaaaa caaatccatg atggatgatg gttggggaga
     2221 aatcatcggt agttggctag aagaagacct acgtttacgc agaaaacttc gtagaacggc
     2281 caaagctatc catgaacaat tgaaaccttt gggtttccct ggctcctatc gaacagtttg
     2341 tgtatacatt tctcaatgga aaaagcagcg ttacgacgaa acgaattacc aagctgtcga
     2401 tcgtcttgat catccgatcg gagaagcaca ggtcgatttt ggaacaatgg aagttatgca
     2461 tgaagatcac atgaaagata tacaacttct tatcatgtca tttccctata gtaatgcgac
     2521 gtccgcaagt gccatgccag ctcaaaacca agagtgtttc ctggaagcat taaaacaact
     2581 atttaatcaa tgcggcggcg ttccaaaaac gatcagaata gataacttaa ctccagcggt
     2641 gaaaaaaaca aaatcaaaat ttgaagaagc acaactaacc gatgaattct tacgtttcac
     2701 cattcattat ggttgtgatg ttcaagtctg taacccacgt agtgggcatg aaaaaggaca
     2761 tgtcgaaaat aaagtgggtt atgtccgata caatttcttc tcgacttcgc caatcatgga
     2821 ctcttttgag tccctaaata agcaacttga agaacaatta aaagcagatc gaaaaaggac
     2881 gcattattca aaacatcaat taatcgaaga tttgtggaag aaagaaaata aagcgttaca
     2941 ttctcttcca caagaggatt acccaatttt taaagaagtg gaagctaaac taaatcgtta
     3001 tcacgaaatg aaattggatc aaacggttat tcatattcct aaagcatatg gctattctca
     3061 attgactgtt gttttgactt ggaataaatt caaagttctt acgcctcatg gagaaatatt
     3121 aatgaacgaa tatcgcccct atatgaacaa aaaaagattg atcccatgga gtgacatcat
     3181 gaaacaatgg agtaataaat tgaatgtcgt tagttattcg aggtattgga agtacttgcc
     3241 tgcacgcctt caaaattatc tagcgattga taataatcac ctgaaataca gacggataaa
     3301 acaattacta agtttattag cgacctacga tatccaacaa attaatgaac aattttacga
     3361 attagtcgtt caagaagacg ccccctctga ctttgatgtg aattggtcaa actatgatgc
     3421 attaacaaaa acaggaggtg tgcaaaaatg attggacaga cgttagaaga ttattgtaaa
     3481 aagcttcgat taagccatat catcacgaac ataccaagag cttctttcga agctccagaa
     3541 caatttatta ccgatttatt ccgtttagaa ctggagacca gacagcagat caaattagaa
     3601 cggatggaaa aattagccaa atttcctgta ccaaaatcgc taaagaacta tgaatggcac
     3661 gaagatattt ttctaccatc tcatctagat aaggagaccc ttgtctctct tgattttcta
     3721 aaaaggcaag aaaacattgt ttgtgttggc tctcctggta cagggaaaac acatctagcg
     3781 atcgcattag ggagaaaagc ctgtagtgaa ggtgtttcaa ccaaattttt ccgagttaac
     3841 aatctcgtag agcaattaga gaatgcatta aaaaaacaac agctagccag tttcaagaag
     3901 cgattcgaaa caacagaatt aattattctt gatgaaatgg ggtacttacc attgacaaaa
     3961 gaaggagctg aacttctctt ccaactgatt tcagattggt atgagcaaaa gagcattatt
     4021 attacctcta atttagaatt cagtcaatgg aatcgagtgt ttatggattc tcgattaaca
     4081 gctgctctcg ttgatcggct aatccatcac gctcacatta tcagcttcaa aggcgaaagt
     4141 ttcagattga aaaattcatt atctaaatca tattgaagga aaaaactagt tgcagcaaaa
     4201 ctctgcactt tcggctgcat ttttttgtaa aaagctattg cacaacacaa acatttgaac
     4261 gacgtaattc caaatgttgg attgctttta gataatcttt tatttattca caattgctaa
     4321 tagtccaatt ttcatttttg tatagtttta atctatactg aaaaacactt gttgttttgg
     4381 acaactcatc aagatactca acaccaacaa tcacttgaat cgctttaccc ttttgtttgt
     4441 aaataggatt cactaattcc acaaatttca aattagtatt aattggttgc atcgcgtctt
     4501 tttcaacata atgctctagt tctttttctg aggctgctgg ataaagcctg aaaaacgtct
     4561 ctaaaaactc ggtgatttct tccgttgtct ttgaatcaat ttttgaatca ctttttactt
     4621 gtcttacttt atgttttgcc ttttctggct taccaatcat agtgggtaaa ctcgttacaa
     4681 tatgattgcc tttttcatct tcaaagatca tcagacgata tgcagaagaa attgattgtg
     4741 tctttttccc ttctgtaatc ttttgatcta ccacaaatga aacgacaaat tcttccgagt
     4801 gtttttcaac atctagaatt tttactgata caacctcgga actagtggga atatccgctc
     4861 gaaccatatc ttggctcaaa gcgagccctt cttctgtaag gtactgctca agattagtca
     4921 ttctcttttc aattacctcc ttgttattct tccatgaaaa atattctttc acaaaatcta
     4981 cagtaaaatt ttctactcca gaggtattca ctattttctc tttcacgacc actttctcat
     5041 gagttgtatg ctgatcaatg gccgtgaagt ttttgtagat tccaaaagca aggctacaag
     5101 acaataaaag ccaaagggca agcaccattt ttcgatgctt gcccacggat actactcgtt
     5161 ctttcttctt ttttggaatc ttttccttgt tacttcgctc aatttttatc ttcattttcc
     5221 attcctcttt tctattgttt ataccgacca gcaccaataa tgtgctgttg ccaatagcta
     5281 tcggtaagat tggtccaacc caccggatct cctgcatgaa acattctgtt ctctcctgca
     5341 taaatgccca catgagtcac ataagtaccg gcatcataag ttgaatgaaa gaaaatcaaa
     5401 tcgcctggtt tggcatcttt taagtcaata tgagtcatag catcatattg atcctgagcc
     5461 acccgaggga gtttcaaacc aactttggca taacaccatt gagttaaacc actacaatca
     5521 aacccagttc cgggacttga cccaccaaat acataagccg ttccttcata cttgaacgcc
     5581 tcgtcaaaaa ttccttggac tgttttatca tcaaactgtg ttgtagttag gtactgcttc
     5641 actaataagc aataaaacat attgccatag ttataccgcc acccactatt ttccttaatc
     5701 gcgatgggat tgggataatc gactttttga ccacccgatt tttcttttga aaaagattcg
     5761 gctaaagcaa aactatgctt ctttccgttt ttcgcgacat aatccaaata cccgtcacca
     5821 tagttataag cctgaatgat actatcttga tcgcatccta gttctttgga acacttaacc
     5881 aaagacgaaa aatagagaca cacttgtttg atggatgcct cggtacttaa tgaattcggt
     5941 gataaacctg ctgattcaga actttgcatg acatcttcca ttgtacctcc agattcgact
     6001 tgaataatcg ccaaaagaat cgaaacatac tctgttatat ctgattcttt ggcatacttt
     6061 tccaccattg aacggtgttt caaaactact tctgagacac tactacctcc aggagaagtt
     6121 ccagaactac cacttccctc accgtcctca ccaaacaaca aacccacaca catcaagatg
     6181 cccacaaaag agatggtcag gaaggttaac gaaataatac tcaatttttt taagttcatc
     6241 ttttctcacc tggctttggt tttgggcgag tgttcttttt tctttgtact tgttgattgg
     6301 ttcgctttgg aaaagtgaat ctctgtttca cttgttgaaa ggaattgttc ttgcttagtg
     6361 gctgagtgct tccgtttgga atgacttctt gactagaaag tttccgtttg atttcaggat
     6421 tcgaatgtac ttgtaccctt ggttttaaag acctattttc tggtagttca tttttctgtg
     6481 gttttatctc cttttttatc ggaacatttt cccttccttt ctcatgacga ttccatgctt
     6541 cgttcaatac tttgcgtttt tcatccattt ttgcgtctct tcgttgtcgt tgtttttctc
     6601 gcaactcccc tcgatttgct ttttcttcga aaggccctcg cttaaagtcc tctggtactt
     6661 ttttcgtctt ttccatgcct ttatgaaggc tgtatttcaa gttagttggt gcatcggtta
     6721 cttgattttt tgcttgtttc gctttgtcct tcacgatttc ttttgtatcg agtacttttc
     6781 caacttttct accaactaaa tttattcgtg attctttctt cgtgcccact ggttgttctt
     6841 gttttttcag atcagaattt agctcgttaa tgcttctcgc ggaatggttc tttctattgg
     6901 acactctatt gatttttgaa ctacctttcc cttttgtaga actggtagct agtttcccac
     6961 caactgctgc accgccacca aaagcaaaag ttcgacccac atttcgatta agctgtcgca
     7021 attttcgatt catcaacatt tgcggtttcc gcatgactcg ccttcccatt gactgactat
     7081 cattactttg aaggttaaac atgctcataa tgtcacctaa tttgaaatag ataccggcaa
     7141 aggtcacaat ttgtagaaaa gctaccataa agaagggata ggtggccgaa atattgaaaa
     7201 acatcgtgga gatactgaat gcagtcgtaa tgactaaggt aactcccgct cgcatcataa
     7261 tcgtattgaa caaacgaata atcgcccttt tgcccaaact ctcatacgtc ggcaacatcg
     7321 acaataagaa actaatgggc agaaacatcg caaagataat gaaaaggatt tgtgagaaca
     7381 acataatccc tgtcagaaga aacacaaaaa aggaaatccc aatattaaac aacactaaaa
     7441 agaccacggt tcctaaacgg ttcatggtct tggtaatact gagattcgca ttgtcattat
     7501 cttcaatctc tgctttgact gcttcttccc gatccttacc cttattttca tccggactca
     7561 cggataaaat cttattaacc cgaccctcgc caatttcttc tatattggaa tcatcgaatt
     7621 gtaatagtaa ccagggttgt tgaacttgaa tcgaaaataa actgtctcga atcaaatcca
     7681 cactatcttt tccctggctt tctgaattag gtacaacaat cttggttcca aggctaagag
     7741 ccgcttcact aacgtctgag ctaaagtcat tgatttttgt aatataggtg ggcgcatacg
     7801 caatgaaaga acccgagagt agaaaaatca tcacaaaatt aatgacggtt cggactgctt
     7861 tggtagtttc tcgttttaat aatccggtat acgccacata aactcctaga gctaaaatca
     7921 tcaataatag gaacccaaag tagaatcccg aagtctgtaa tccattttca gtgataccgg
     7981 ctaaagtttg gatattcttt ccaatacttt tagccgtatc cgaaatgaaa tccaacttgt
     8041 aagcttcttg aaccacatag cccgtggcgt ttgataaata cagacttact gtccaaataa
     8101 aattggtaat ggcataaaga ccatacatca cgcttttccc taagccatcc ccccaattcc
     8161 aaggtaacca atcccaagaa ctatccacaa agaaatccag ttgataattg ttcagagaat
     8221 atttggaata aaggtttccg gcttcaactg tatcatcaac cagacctgtc gcctcagcta
     8281 ccgtaccgac taaactcaat agaactgtca ataggacgat gctaataaaa acgattccca
     8341 caatccgaaa aagtttcttt ttcatcccct cactccattc tctcttgaac cggcggacga
     8401 gtgtcaaaag catggaagag ttcttcaaag actggatgaa cttgaaccac acctactcgc
     8461 ccatacaagt cttgcatcaa gcattgaccg tttt
//
LOCUS       DAZKLM010000106         8300 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3764
            SAMEA115706913-rid22582563.denovo.110, whole genome shotgun
            sequence.
ACCESSION   DAZKLM010000106 DAZKLM010000000
VERSION     DAZKLM010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706913
            Sequence Read Archive: ERR13196647
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8300)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8300)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287823.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287823.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,004
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,849
            CDSs (with protein)               :: 2,849
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 41 of 96
            Pseudo Genes (incomplete)         :: 52 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 15 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8300
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706913-rid22582563.denovo.110"
                     /isolate="VRE3764"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..429)
                     /locus_tag="ACG842_002400"
     CDS             complement(<1..429)
                     /locus_tag="ACG842_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0033745 - L-methionine-(R)-S-oxide
                     reductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HFQ0248902.1"
                     /translation="MWTEKQKKDAYEILLLQQQGLLEAEDNWLANLANSSALLNETLP
                     ETVFAGYYLFDGNELILGPFQGRVSCTRIKMGKGVCGESAEKQETLIVDDVKTHANYI
                     SCDSAAQSEIVVPMVKEGNLIGVLDIDCGVTKGYDEIDQAF"
     gene            complement(559..1269)
                     /gene="budA"
                     /locus_tag="ACG842_002401"
     CDS             complement(559..1269)
                     /gene="budA"
                     /locus_tag="ACG842_002401"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047605 - acetolactate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0045151 - acetoin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HFQ0248903.1"
                     /translation="MTEKILYQHGTLGALMAGLMDGTETIAHILEKGTLGLGTLHGLD
                     GEVIFLDGVAYQGRSDGSVAQLDGSELTPYAAITDFTPDTSFSVSKTADGEQLKKDIL
                     VREAGENLFLAVKITGLFKNMHIRIMPKQEKPYRRLAKISESQPEFQQSNVQGTLVGF
                     FTPELFQGVAAAGFHLHFIDDTHTFGGHVMDFEVAEGKVEISRISSLVQHFPVDDPTF
                     VHETIDYADLAAEIQQAE"
     gene            complement(1282..2946)
                     /gene="alsS"
                     /locus_tag="ACG842_002402"
     CDS             complement(1282..2946)
                     /gene="alsS"
                     /locus_tag="ACG842_002402"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0034077 - butanediol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase AlsS"
                     /protein_id="HFQ0248904.1"
                     /translation="MDEKMKQGSKSVVESLINHHVDYVFGIPGAKIDGVFNELEDQGP
                     ELIVTRHEQNAAFMAQAIGRITGEPGVVIATSGPGASNLATGLVTATAEGDPVLAIAG
                     QVKRSDLLKLTHQSMDNAALFQPITKYSAEIQDPETISEVIANAYRMAKSSKKGASFI
                     SIPQDVVDAPVQGNVIKPLSDPKLGSASADDIRYLAERIREAKLPVLLVGMRGSSEKE
                     TLAIRQLVGKTALPVVETFQAAGVISRELEAHFFGRVGLFRNQPGDMLLKRSDLVIAI
                     GYDPIEYEARNWNAEKDARIIVIDEAPAEIDPFMQPERELIGDISATLDLLTGSLEPQ
                     QVSEDAKEYLASLQAELTERDIVQSKGEAGILHPLEVINTLQSKVTDDMTVTVDVGSH
                     YIWMARHFRSYEPRHLLFSNGMQTLGVALPWAISAALVRPNTQIVSVSGDGGFLFSAQ
                     DLETAVRKKLNIVHLIWNDGHYNMVEFQEKMKYQRASGVDFGPVDFVKYAEAFGAKGI
                     RATSVEELEKALEEGFATEGPVIIDIPIDYRDNEKLGETILPDQFY"
     gene            complement(3151..3813)
                     /locus_tag="ACG842_002403"
     CDS             complement(3151..3813)
                     /locus_tag="ACG842_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HFQ0248905.1"
                     /translation="MKEQRFDKNVYMEFFYWLYQSLASSLCFWCVNILYIAAAFLLSI
                     DGRNVFSFIFSLLFIGPGCIVMIGMTEELAKEKSFSPVRLFIKTFRQFWLKGFFYWLF
                     AWIVSVIMIFDCFFFIRFSYGKWLIPLFVLLACLSVSFSINYWYFQVRNPASKPNQVL
                     RIAFYYTLKKWYVSLLDFLLLTSLFLFFFVKPQWCILLGPSIVFGLIYFNNRKLMRTM
                     DL"
     gene            complement(3823..4638)
                     /locus_tag="ACG842_002404"
     CDS             complement(3823..4638)
                     /locus_tag="ACG842_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0248906.1"
                     /translation="MPFIQWLGMILLIVLALLTIIPFLWMVSASFKANNEVFTIPIQW
                     IPKTWHLENYLVIWSRIPLVTFFKNTAFLSVVITLIQLFTSSFAAYGFSKLNFKGRDI
                     LFLAYIGTIAVPWQSYMIPQFIMMRKVGLTYTLWSLVLLQAFNAFGVFLLKQYYSSIP
                     DSLCESARIDGLSEWGIYRKIILPLTKPAIASLTIITFVNTWNDYMGPFIYLSSTENK
                     TIQLGLKMFIGLFDAEYALIMAASVVSVLPVAFIFLAMQKYFVEGIASSGMKE"
     gene            complement(4900..5343)
                     /locus_tag="ACG842_002405"
     CDS             complement(4900..5343)
                     /locus_tag="ACG842_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator
                     substrate-binding protein"
                     /protein_id="HFQ0248907.1"
                     /translation="MLDPVDTEKYETILLPRKEKWGLLILENSVLAKKDVITKEDFAN
                     VPLLISKRPEIQRMFADWLGIKKEQLLITGTYNLIFNVFALIENQVGAALVIEGAVSH
                     LSKRVTFVPLDPMIETNCMLVWKKNRILSPVVNELIHRFKEAFEQ"
     gene            complement(5477..5815)
                     /locus_tag="ACG842_002406"
     CDS             complement(5477..5815)
                     /locus_tag="ACG842_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFQ0248908.1"
                     /translation="MYQVKKSRSIAMELRLLKYFWTIAEEGTISQAAEVLHLTQPTLS
                     RQLKELEEELGTELFVRENRKMILTEAGYFLKKPSRRNFRFNESNHSRIREQKKTIIQ
                     RPIYDRLCGS"
     gene            complement(5803..6180)
                     /locus_tag="ACG842_002407"
     CDS             complement(5803..6180)
                     /locus_tag="ACG842_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFQ0248909.1"
                     /translation="MNIKQVSEEKGISADTLRYYERIGLIPPVNRTKGGIRDYTEEDL
                     KWVDFTICMRNAGLSIESLIEYIRLSSEGEATVFERRKLLIEESELLTKEIAEMQECL
                     ERLQGKIKKYDRVLSRNKMECIK"
     gene            6404..7597
                     /locus_tag="ACG842_002408"
     CDS             6404..7597
                     /locus_tag="ACG842_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFQ0248910.1"
                     /translation="MTTYQRKVLLSTSAGIALENMDIMFLAFSLSSMITSFHISGTQA
                     GFISTITNLGMLIGGIFFGIIADKYGRVKVFSQTVLLFSIASLLMYFASNIYLVYLFR
                     FIAGVGAGGEYGACMSLISESFSKKQIGRASSVAGIGAQVGAALAAILAAVIIPTFGW
                     KMLYVVGVLPVLMVLFIRRGLTEPKEFKSVQSAEKKTKLSRLFESKHLAWQTCGLSIM
                     VTVQIAGYFGLMNWLPSIMQNRLGLSVSGSSLWMVSTILGMSLGMMTFGIIMDKLGPK
                     LAFSLFLICSSLSVYLLVLAHSQWSLVAAAVIVGYFINGMYGGYGAVISSLYPTEIRA
                     TANNFIMNLGRAVGGFSSIVIGFLMDHYSLTAVILFLSGIYLVSLLVLTTLTGVTALR
                     ESLQH"
     gene            complement(7763..8185)
                     /locus_tag="ACG842_002409"
     CDS             complement(7763..8185)
                     /locus_tag="ACG842_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0248911.1"
                     /translation="MQTFLTKVSQKKIKMIHLLLETERWCSIEELQNELKVSSKSILN
                     YLTDLEELFQKYPDKVILKNENNRRFSMEKQENFPIYIIYLHFYRKSYNYHLIEFMYQ
                     HPEKNLEDYADAQYTSVSTVFRYAKLLVKYFEMTDVKE"
BASE COUNT         2428 a         1688 c         1405 g         2779 t
ORIGIN      
        1 aaatgcttga tcgatttcat catacccttt cgttactccg caatcgatat ctaaaacacc
       61 gattaaatta ccttctttta ccattggcac tacgatttct gattgtgcgg ctgaatcgca
      121 ggaaatgtaa tttgcatggg ttttgacatc atcaacgatc aatgtttctt gtttttcagc
      181 tgattctcca caaactcctt ttcccatctt gatacgtgtg caagaaactc gcccttggaa
      241 cggtcctaaa atcaattcat taccatcgaa taaataatat cctgcaaaga cagtctcagg
      301 caaagtctca ttcaacaatg cagatgaatt tgctaaattt gctaaccagt tatcttctgc
      361 ttctaataat ccttgttgct gtaataataa aatttcgtat gcatcttttt tctgtttttc
      421 tgtccacatg ataaaaaccg ctcctttttg atctatggag aagattatag ctcagttttt
      481 ttgtaaacgc taggtttcct cctcaaaaaa acgagatacg tcaggttaat caccttgaac
      541 gcatctcgct gtctttttct attctgcttg ttggatttct gctgctaaat cagcataatc
      601 aatcgtttcg tgaacaaaag tcggatcatc aactggaaaa tgctgtacaa gggacgagat
      661 gcggctaatc tcaacttttc cttcagccac ttcaaaatcc attacatgtc cgccaaatgt
      721 atgtgtatca tcgataaaat gcaagtgaaa tcccgctgca gcaactcctt ggaataattc
      781 aggcgtaaag aacccgacta atgttccttg gacattgctt tgttggaatt ctggttgaga
      841 ctctgaaatt tttgctagtc ttctgtaagg tttttcttgc ttcggcatga tacggatgtg
      901 catatttttg aataatccag ttatcttcac tgccaaaaat aggttttccc cggcttctct
      961 tactaatatg tcttttttca attgttctcc atctgcagtt tttgagacag aaaaactagt
     1021 atctggcgta aaatctgtaa ttgctgcata gggggtaagt tcacttccat ctaattgggc
     1081 aactgagcca tctgatcttc cttgataagc tacgccatct aaaaagatga cttcaccatc
     1141 aagcccatgc aatgttccca atcccagagt tcctttttct aatatatgag caatcgtttc
     1201 tgttccatcc atcaagccag ccatcaaagc tcctaatgtc ccgtgctggt ataagatttt
     1261 ttcagtcatg tcgacacttc cttaatagaa ttgatctggt aagattgttt ctcccaattt
     1321 ctcattatca cggtaatcaa tcggaatatc aataattacg ggtccttctg tcgcaaatcc
     1381 ttcttctaaa gctttttcta attcctctac acttgtagct cggattcctt tagcgccaaa
     1441 agcttctgca tatttcacaa aatcaactgg accaaagtct acacctgacg cccgctgata
     1501 tttcattttt tcttggaact ctaccatatt gtagtgtcca tcattccaaa tcaaatgaac
     1561 gatattcaat tttttacgta ccgccgtttc taaatcctgg gcagagaata aaaatccccc
     1621 atcgccggaa actgatacga tttgtgtatt tggacgcacc aatgcagcag aaatcgccca
     1681 tggcaatgca acaccaagcg tttgcatacc attactgaat aacaaatgac gaggttcata
     1741 acttctaaag tggcgtgcca tccaaatata atgactacct acatctacgg taacagtcat
     1801 atcatctgtt acttttgact gaagcgtgtt aataacttct aaaggatgca agatacctgc
     1861 ttctcctttt gattgaacaa tgtcccgctc cgttaattct gcttgaagag atgctaaata
     1921 ttcttttgca tcttctgaca cttgttgagg ctctaatgaa cctgtcaata aatcaagagt
     1981 ggctgagata tcaccgatca attctctttc aggctgcata aaaggatcga tttcagctgg
     2041 cgcttcatcg atcacaatga tccgcgcatc tttttccgca ttccagtttc ttgcttcata
     2101 ttcgattgga tcatagccaa tagcaataac tagatcactt cgtttcaaaa gcatatctcc
     2161 tggttggttt ctaaataaac ccacacgacc aaagaaatgc gcttctaatt cacgtgagat
     2221 cactccagct gcttggaatg tctctacaac aggcaatgct gtctttccaa ctaattgcct
     2281 gatggctaac gtttcttttt cacttgatcc tcgcatacct actaatagta ccggtagttt
     2341 tgcttctcta atgcgttcag ctaggtaacg gatatcatct gcagaagcag aacctaattt
     2401 aggatcgctc aatggcttga tgacattccc ttggacaggt gcatccacta catcttgcgg
     2461 gatactgata aagctcgcgc ctttttttga actcttagcc atacgataag cattggcaat
     2521 cacttctgaa attgtttctg gatcttggat ttctgcactg tattttgtga ttggctggaa
     2581 cagtgctgca ttatccatgc tttgatgggt caacttcaat agatcgcttc gttttacttg
     2641 tccggcaatt gctaaaactg ggtcaccttc tgctgtcgct gttaccaaac ctgtagctaa
     2701 gttactagca ccaggaccac ttgttgcgat aactactcct ggctctcctg taatacgtcc
     2761 gattgcttgt gccataaatg cagcattttg ttcgtgacga gtcacgatta attcaggacc
     2821 ttgatcctct aattcattaa atacaccatc gatttttgca cctggtatac cgaatacata
     2881 atcaacatga tgattaatca aactttctac tacagatttc gaaccttgtt tcattttttc
     2941 gtccattgaa catcgtcctt tttatgcttt atttttaatt attagttaga aataatcaca
     3001 aataattgat acagtttcta aacctgtttc attcaaaagt atagcacgtt ttatttcatt
     3061 tgtttagcca tatgtttcaa aaaatgaaaa agaatgacag tttttacata aaaactgtca
     3121 ttcttaattg aaagtttcaa tttataaagt ctacagatcc atagttctca tcaatttacg
     3181 attgttgaag taaatcaaac caaatacgat agaaggacct agtagaatac accattgggg
     3241 ctttacgaaa aagaagagaa acaaacttgt caatagtaaa aaatctagta aactcacata
     3301 ccattttttc aacgtatagt agaacgctat ccgtagaact tgatttggtt tcgacgctgg
     3361 atttctcacc tgaaaatacc agtagttgat gctaaaggaa actgataaac aagcaagtaa
     3421 aacaaacaac ggaatcaacc attttccata agaaaaacgt ataaaaaaga aacaatcaaa
     3481 aatcattatc acactaacta tccaagcaaa tagccaataa aaaaatccct ttagccaaaa
     3541 ttggcgaaac gtctttatga acaaacgtac tggtgaaaaa cttttttctt ttgctaattc
     3601 ttctgtcata cctatcatca cgatgcaacc tggaccaata aaaagtaaag aaaaaataaa
     3661 ggaaaagaca ttccttccat ctatcgacaa aaggaacgca gctgctatat acaagatatt
     3721 gacacaccaa aaacaaaggc tacttgccaa tgattgatac aaccaataaa agaattccat
     3781 ataaacattt ttgtcaaaac gctgctcttt cataatcacc tcctattcct tcattcctga
     3841 tgaagcgatc ccttctacaa aatatttttg catcgctaaa aatataaaag caacaggaag
     3901 aacagaaaca actgaggcag ccatgattaa tgcgtactct gcatcaaata gcccaataaa
     3961 cattttcaac cctaactgga ttgttttatt ttccgtagaa gacaaataaa tgaatggccc
     4021 catataatcg ttccatgtat taacgaatgt gatgatcgtc aaactggcaa tcgccggttt
     4081 tgtaagaggt aaaatgatct tgcgatagat tccccactct gataagccat cgatccgcgc
     4141 actctcacat aaagaatctg ggatactact gtagtattgc ttcaacaaga acacaccaaa
     4201 agcattgaac gcttgtaata gcaccaacga ccacaacgta tatgttaaac ccacttttct
     4261 catcataatg aactgcggga tcatataaga ctgccaaggg acagcaatcg tgccgatata
     4321 tgctaaaaat aaaatatcgc gccctttaaa atttagttta gaaaacccgt aagcagcaaa
     4381 actagaagta aacaactgaa tcaacgtgat cacaacgctt aaaaaagctg tattcttaaa
     4441 aaatgtcact aaaggaatac gagaccaaat aaccagataa ttctctaaat gccaagtttt
     4501 agggatccat tgaatcggaa tcgtgaacac ttcattattt gctttgaatg aagcagaaac
     4561 catccataaa aaaggaatga tcgtcaataa agctaataca atcagcagaa tcatacccaa
     4621 ccattgaata aagggcatgt tttttgaacg aatttttgct ttttttcttt catttcgaac
     4681 acctccaaag ttataatctt catttctttg taatgattac aatccagaac aacggttatt
     4741 atagctgatt catttccgtt cttcttatgt ttgtaagcgc ttatttaaca agttatgtaa
     4801 atctctttat tttttttgat ttaaaaatga aaaaatacaa aagcaagact gtcatacgtc
     4861 tcgcttttgt attgaaattt cttcgattag agatacttat tactgttcaa aagcttcttt
     4921 gaaacggtga atcaattcat tgactacagg tgataaaatc cgattctttt tccagactag
     4981 catgcagttc gtttcaatca ttgggtcaag cggaacgaat gtcactcgct ttgaaagatg
     5041 acttacagca ccttctatga ctaaagcagc acctacctga ttctcaatta gcgcaaatac
     5101 attgaaaatc aaattgtacg ttcctgtgat cagcaactgc tcctttttta tgcctaacca
     5161 gtcagcaaac attcgctgta tttctggacg ctttgagatc aaaagaggta cattcgcaaa
     5221 gtcttctttt gttatcacgt ctttttttgc taaaacagaa ttttctaaaa tgagcaatcc
     5281 ccatttttcc tttcttggta acaaaatcgt ttcgtatttc tctgtatcta ctggatctag
     5341 caaaatcgct aaatctaaca ggcttttttc tagtcactcc gtgatatcat cacttgttcc
     5401 gctgtggata tgaaattgaa cgctgggata atcacggatc aacttttcta gtaatagtgc
     5461 caatgtattc gaattatcag cttccacaca gccgatcgta aatcggccgc tgaataattg
     5521 tttttttctg ttctcgaatt cttgagtggt ttgattcgtt aaatctaaaa tttcttctgc
     5581 tcggcttttt tagaaaataa cctgcttctg tcaagatcat tttgcggttt tcgcgtacaa
     5641 acagttctgt tcctagttcc tcttctaact ccttcagttg tcggcttaag gttggttgtg
     5701 taagatgtag aacttctgcg gcttgagaga ttgttccttc ttctgcaatt gtccaaaaat
     5761 attttagtaa gcgtaactcc atagcgatgc tccttgattt ttttacttga tacattccat
     5821 tttatttcta gataacacac gatcatattt cttgattttt ccttgtaaac gttccaaaca
     5881 ctcttgcatt tcagcaattt ctttcgtcaa aagttcactt tcttcaatca aaagcttacg
     5941 ccgctcaaat acagttgctt ctccttcgct agataggcga atatattcaa tcaaagactc
     6001 gatcgataat ccggcatttc gcatacagat cgtaaagtcg acccatttta ggtcttcttc
     6061 tgtataatca cggattccgc cttttgtccg attaactggt ggaatcaacc cgattcgttc
     6121 ataatagcgc aatgtatcgg ccgaaatgcc cttttcttca ctgacttgtt tgatattcat
     6181 ctgatcactc tttctcttaa tttcttgcca ctttacttat gtattttatt tattctacaa
     6241 ctttaagtgt actccaagtc aatactcatt tctattaaaa ctaagctgga ttatctatat
     6301 cggttgtttg tgtattattt caaataaaaa agtcttgttt ttttattctt ttttggatat
     6361 acttttaaca atttagggaa attttaggaa aaggggtata aaaatgacaa cgtatcagcg
     6421 aaaggtatta ttatcgacat cggcgggaat tgctttagag aatatggata ttatgttttt
     6481 agctttttca ttatcttcaa tgattacttc ttttcatatt agtggtacgc aagcgggttt
     6541 catttcaacg atcactaatt tggggatgct cattggaggt attttttttg gcatcatagc
     6601 tgataaatat ggacgtgtga aagtattttc tcaaacggtc cttctgtttt cgattgcttc
     6661 tttattgatg tattttgctt caaacatcta cttggtttat ctctttcgct ttatcgcagg
     6721 tgtaggagcg ggaggagaat acggggcgtg tatgtccttg atttccgaaa gtttttcaaa
     6781 aaaacaaatt ggacgagctt cttcggttgc tggtattggt gcgcaggtcg gtgcggcttt
     6841 ggctgcgatt ttagctgctg tgatcattcc aacgtttggt tggaagatgt tgtatgtcgt
     6901 aggtgtactg ccggtgttga tggtcttgtt cattcgtcgc ggcttgactg aaccaaagga
     6961 attcaaatct gtacaatctg ccgaaaagaa aaccaaactt agcagactgt ttgaatcaaa
     7021 acatcttgct tggcaaacat gtggccttag tatcatggta actgtccaaa ttgccggcta
     7081 ttttgggtta atgaattggc tgccttcaat tatgcaaaat cgattagggc tttcggtttc
     7141 tgggtcttca ttgtggatgg tcagtactat tttaggcatg tctttgggga tgatgacttt
     7201 tggcataatc atggataaat tggggcctaa actagccttt tctttgtttt taatttgttc
     7261 atccttatct gtctatctac ttgtgttagc ccatagccag tggagtttgg tagcagccgc
     7321 tgtgatagtc ggctacttta tcaatggcat gtatggtgga tatggcgcag tgattagtag
     7381 tttgtatcca acagaaatcc gcgcgacggc gaataatttc attatgaacc ttggacgagc
     7441 tgttggtggt ttttcttcta tcgtcatcgg tttcttgatg gatcattata gtttaactgc
     7501 cgtaatcttg tttttgagcg gtatttacct agtcagtctg ctggtcttga caactcttac
     7561 tggtgttaca gcattaaggg aaagcttgca gcattgatag ttgagtctgt aaatgacaag
     7621 aaagaggtgt ggcaaaagct tgcatctgcg gtaataagac cagccaaatc aaaaacatga
     7681 gacactgttt ccgtttggct ggtcgtatta cttgatgcag ggcagctttt tcaactgcta
     7741 atttccttct atctggcggc gactattctt tgacgtccgt catttcaaag tactttacta
     7801 ataacttagc ataacgaaaa actgtggata cactcgtata ttgagcgtct gcataatctt
     7861 ccaaattttt ctcaggatgt tgatacatga attcaatcaa atgataatta tatgattttc
     7921 tgtagaaatg aagataaata atgtagattg ggaagttctc ctgcttttcc atagaaaagc
     7981 gccgattatt ctcattcttc aaaatgacct tatcaggata tttttggaac aattcttcta
     8041 aatctgttag ataattcaag atagacttac ttgatacctt caattcgttt tgtagttctt
     8101 ctattgaaca ccaacgttct gtttcaagca gtaaatgaat cattttaatt ttcttttgag
     8161 aaacttttgt taaaaaagtc tgcatatttt ctcctcctgt atctacttta ctatactaca
     8221 tacaagaaaa aaaacggata caaatatcat attgtcacac cgtcaatagt gcataaacct
     8281 gttattattt ctcttttttt
//
LOCUS       DAZKLM010000107         8065 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3764
            SAMEA115706913-rid22582563.denovo.111, whole genome shotgun
            sequence.
ACCESSION   DAZKLM010000107 DAZKLM010000000
VERSION     DAZKLM010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706913
            Sequence Read Archive: ERR13196647
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8065)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8065)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287823.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287823.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,004
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,849
            CDSs (with protein)               :: 2,849
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 41 of 96
            Pseudo Genes (incomplete)         :: 52 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 15 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8065
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706913-rid22582563.denovo.111"
                     /isolate="VRE3764"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..517
                     /locus_tag="ACG842_002410"
     CDS             <1..517
                     /locus_tag="ACG842_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CatA-like O-acetyltransferase"
                     /protein_id="HFQ0248912.1"
                     /translation="KGYEIYPSLIYAIMEVVNKNKVFRTGINSENKLGYWDKLNPLYT
                     VFNKQTEKFTNIWTESDKNFISFYNNYKNDLLEYKDKEEMFPKKPIPENTIPISMIPW
                     IDFSSFNLNIGNNSSFLLPIITIGKFYSENNKIYIPVALQLHHSVCDGYHASLFMNEF
                     QDIIHRVDDWI"
     gene            complement(1210..2148)
                     /locus_tag="ACG842_002411"
                     /pseudo
     CDS             complement(1210..2148)
                     /locus_tag="ACG842_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108743.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
     gene            complement(2148..2765)
                     /locus_tag="ACG842_002412"
     CDS             complement(2148..2765)
                     /locus_tag="ACG842_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFQ0248913.1"
                     /translation="MAKIGYARVSSKEQNLDRQLQALQGVSKVFSDKLSGQSVERPQL
                     QAMLNYIREGDIVIVTELDRLGRNNKELTELMNQIQIKGATLEVLNLPSMNGIEDENL
                     RRLINSLVIELYKYQAESERKKIKERQAQGIEIAKKKGKFKGRQHKFKENDPRLKHAF
                     DLFLNGLSDKEVEEQTGINRRTFRRYRARYNVTVDQRKNNEKRDS"
     gene            complement(2779..2949)
                     /locus_tag="ACG842_002413"
     CDS             complement(2779..2949)
                     /locus_tag="ACG842_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000713595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0248914.1"
                     /translation="MKKFIYRVLENDEVVAIFNEQQYAQDFIAYEKTISDKQFEIEKV
                     DIADWLLQPREF"
     gene            complement(3297..4787)
                     /locus_tag="ACG842_002414"
     CDS             complement(3297..4787)
                     /locus_tag="ACG842_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015543625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primase C-terminal domain-containing protein"
                     /protein_id="HFQ0248915.1"
                     /translation="MNIPFVVETVLHDGLLKYKFKNSKIRSITTKPGKSKGAIFAYRS
                     KSSMIGGRGVVLTSEEAIQENQDTFTHWTPNVYRYGTYADENRSYTKGHSENNLRQIN
                     TFFIDFDIHTAKETISASDILTTAIDLGFMPTMIIKSDKGYQAYFVLETPVYVTSKSE
                     FKSVKAAKIISQNIREYFGKSLPVDLTCNHFGIARIPRTDNVEFFDPNYRYSFKEWQD
                     WSFKQTDNKGFTRSSLTVLSGTEGKKQVDEPWFNLLLHETKFSGEKGLIGRNNVMFTL
                     SLAYFSSGYSIETCEYNMFEFNNRLDQPLEEKEVIKIVRSAYSENYQGANREYITILC
                     KAWVSSDLTSKDLFVRQGWFKFKKKRSERQRVHLSEWKEDLMAYISEKSDVYKPYLVT
                     TKKEIREVLGIPERTLDKLLKVLKANQEIFFKIKPGRNGGIQLASVKSLLLSIIKVKK
                     EEKESYIKALTNSFDLEHTFIQETLNKLAERPKTDTQLDLFSYDTG"
     gene            complement(4830..5129)
                     /locus_tag="ACG842_002415"
     CDS             complement(4830..5129)
                     /locus_tag="ACG842_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0248916.1"
                     /translation="MNHDECKTYIKNSLLEIRKLANIYTLETFKKELEKRNIYLETKS
                     DKYFSSEGEDYIYKLIENNKIIYSISGKKLTYKGKKSFSKHAILKQLNEKANQVN"
     gene            complement(5495..5779)
                     /locus_tag="ACG842_002416"
     CDS             complement(5495..5779)
                     /locus_tag="ACG842_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFQ0248917.1"
                     /translation="MELAFRESLKKMRGTKSKEKFSQELEMSRSNYSRIESGKSDPTI
                     KTLEQIAKLTNSTLVVDLIPNEPTEPEPETESEQVTLDLEMEEEKSNDFV"
     gene            complement(6298..6783)
                     /gene="vanZ-A"
                     /locus_tag="ACG842_002417"
     CDS             complement(6298..6783)
                     /gene="vanZ-A"
                     /locus_tag="ACG842_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000516404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance protein VanZ-A"
                     /protein_id="HFQ0248918.1"
                     /translation="MGKILSRGLLALYLVTLIWLVLFKLQYNILSVFNYHQRSLNLTP
                     FTATGNFREMIDNVIIFIPFGLLLNVNFKEIGFLPKFAFVLVLSLTFEIIQFIFAIGA
                     TDITDVITNTVGGFLGLKLYGLSNKHMNQKKLDRVIIFVGILLLVLLLVYRTHLRINY
                     V"
     gene            complement(6936..7847)
                     /locus_tag="ACG842_002418"
     CDS             complement(6936..7847)
                     /locus_tag="ACG842_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala carboxypeptidase VanY-A"
                     /protein_id="HFQ0248919.1"
                     /translation="MKKLFFLLLLLFLIYLGYDYVNEALFSQEKVEFQNYDQNPKEHL
                     ENSGTSENTQEKTITEEQVYQGNLLLINSKYPVRQESVKSDIVNLSKHDELINGYGLL
                     DSNIYMSKEIAQKFSEMVNDAVKGGVSHFIINSGYRDFDEQSVLYQEMGAEYALPAGY
                     SEHNSGLSLDVGSSLTKMERAPEGKWIEENAWKYGFILRYPEDKTELTGIQYEPWHIR
                     YVGLPHSAIMKEKNFVLEEYMDYLKEEKTISVSVNGEKYEIFYYPVTKNTTIHVPTNL
                     RYEISGNNIDGVIVTVFPGSTHTNSRR"
BASE COUNT         2435 a         1456 c         1206 g         2968 t
ORIGIN      
        1 gaaaggatat gaaatttatc cctctttaat ttatgcaatt atggaagttg taaataaaaa
       61 taaagtgttt agaacaggaa ttaatagtga gaataaatta ggttattggg ataagttaaa
      121 tcctttgtat acagttttta ataagcaaac tgaaaaattt actaacattt ggactgaatc
      181 tgataaaaac ttcatttctt tttataataa ttataaaaat gacttgcttg aatataaaga
      241 taaagaagaa atgtttccta aaaaaccgat acctgaaaac accataccga tttcaatgat
      301 tccttggatt gattttagtt catttaattt aaatattggt aacaatagca gctttttatt
      361 gcctattatt acgataggta aattttatag tgagaataat aaaatttata taccagttgc
      421 tctgcaactt catcattctg tatgtgatgg ttaccatgct tcactattta tgaatgaatt
      481 tcaagatata attcataggg tagatgattg gatttagttt ttagattttg aaagtgaatt
      541 taattttata cacgtaagtg atcataaaat ttatgaacgt ataacaacca cattttttgg
      601 ttgcttgtgg ttttgatttt gaatttggtt ttgaacttat ggactgattt attcagtcca
      661 ttttttgtgc ttgcacaaaa actagcctcg cagagcacac gcattaatga cttatgaaac
      721 gtagtaaata agtctagtgt gttatacttt acttggaaga tgcaccgaat aaaaaatatt
      781 gaagaacaac tagcaaaaga ttttaaagag ttattttatt ttaagtcttt ataacatgag
      841 tgaagcgaat ttttaaattt cgatagaaat ttttacatca aaaagccccc tgtcaaaatt
      901 gacgaagggg gttttttggc gcacgctttt cgttagaaat atacaagatt gaaaatcgtg
      961 tataagtgcg ccctttgttt tgaacttagc acgttacatc aattttttaa aatgatgtat
     1021 aagtgcgccc ttttaaattt tgagtgatta tattttttga gttagaaaaa gggattggga
     1081 aaatttccca aaataattta aaaaataagc aaaaattttc gatagagaat gtgctatttt
     1141 ttgtcaaagg tgtatacctt gactgtgctt gctgttacat taagtttatt tttaagttat
     1201 taaaaaagaa atagctttta aagtttggct cgctgtcgct ttataaagct gattgacttt
     1261 tgattgcaaa ctacttaaag aaaacaaact cggactattc gttttcttct ctttggtttg
     1321 aacatcagca attatcccct cttgattgcc tattttagct tgtttagaag aaacaaaagc
     1381 taaaagctcc tcttgggttt taaaacgctg tgtggggctt agaacgccct taaacgaccc
     1441 ttggtttact tttatactag cttccacctc gaaaaaaggt tcttttttaa aattctctat
     1501 ggcttcctgg cgctgaaaaa taaggtataa ggtgggcgtt tgaacacgtc ctagtgaaaa
     1561 tgtaccttgt acgcccttct gttgtaaatt taacgtatac aaagggcttg cgttcatgcc
     1621 gatcaaccaa tcggcaattt ggcgtgtttg cgcttcttga taaaagggat agtaattcat
     1681 tccaggttgc aaattttgaa aaccgcttcg gattacatct ttttctaagc tattgatcca
     1741 tagtctttta aatgttttat cttttgaaaa ggcatttgct ttatggataa tcgaccaggc
     1801 gatattttca ccttctctgt cgctatctgt tgcaacaata attgtatttg cctttttgag
     1861 aagttctgca acaattttaa actgctttcc cttatctttt gcaacttcaa aatcgtatcg
     1921 atcaggaaaa atcggcaaag attcaagttt ccaattttgc cacttttcgt cataatgacc
     1981 tggttctgct aattccacta aatgcccaaa accaaaggtg ataaacgttt catctgtaaa
     2041 tagtgggtct ttgatctcaa aataaccgtc ttttttggtg ctttgtttta aagcacttgc
     2101 gtaggctaat gcctggcttg gtttttcagc taaaataacc gtactcatta actatccctc
     2161 ttttcattgt tttttctttg atcgactgtc acgttatatc ttgctcgata ccttctaaac
     2221 gttcggcgat tgattccagt ttgttcttca acttctttat cggataaacc attcaaaaac
     2281 aaatcgaaag catgctttaa acgtggatca ttttctttaa atttatgctg acgacctttg
     2341 aatttgcctt ttttcttagc aatttcgatt ccttgtgcct gacgttcctt aatttttttt
     2401 cgttctgatt ctgcttgata cttgtacaat tcaatgacaa ggctattaat caaacgcctt
     2461 aaattttcat cttcaatacc attcattgag ggtaaattta agacttccag ggttgccccc
     2521 ttaatttgaa tttgattcat caattctgtt aattctttat tatttcgtcc taatcgatct
     2581 aattcagtaa caataacaat atccccttca cgaatatagt taagcatagc ttgtaattgt
     2641 gggcgttcga ccgattgacc gcttaatttg tctgaaaaga ccttagaaac gccctgtaac
     2701 gcttgtaatt gccgatctaa gttctgttct ttgctactga cacgtgcata accaatttta
     2761 gccattttca accaacctct aaaattctct cggttgcaat aaccaatcag caatatctac
     2821 tttttcaatt tcaaattgct tatcagaaat tgtcttttcg taagcgataa aatcttgcgc
     2881 atattgttgc tcattaaaaa tagccaccac ttcgtcattt tctaaaactc gataaataaa
     2941 ttttttcatt ttactcctcc tattatgccc aacttaaatg acctattcac caagtcaatt
     3001 atactgctaa aatcatatta ggacaaatag gtatactcta atgacctata aaagatagaa
     3061 aattaaaaaa tcaagtgttc gcttcgctct cactgcccct cgacgtttta gtagcctttc
     3121 cctcacttcg ttcagtccaa gccaactaaa agttttcggg ctactctctc cttctccccc
     3181 taataattaa ttaaaatctt actctgtata tttctgctaa tcattcgcta aacagcaaag
     3241 aaaaaacaaa cacgtatcat agatataaat gtaatggcat agtgcgggtt ttattttcag
     3301 cctgtatcat agctaaacaa atcgagttgt gtgtccgttt tagggcgttc tgctagcttg
     3361 tttaaagtct cttgaatgaa tgtatgctct aagtcaaaag aatttgtcag cgcctttata
     3421 tagctttctt tttcttcttt ttttacttta atgatcgata gcaacaatga tttaacacta
     3481 gcaagttgaa tgccaccatt tcttcctggt ttaatcttaa agaaaatttc ctgattcgcc
     3541 ttcagtacct tcagcaattt atctaatgtc cgttcaggaa tgcctagcac ttctctaatc
     3601 tcttttttgg tcgtcactaa ataaggcttg tatacatcgc ttttttcgct aatataagcc
     3661 attaaatctt ctttccattc tgacaaatga acacgttgac gttcgcttct ttttttcttg
     3721 aatttaaacc acccttgacg gacaaataaa tctttactgg ttaaatcact tgatacccaa
     3781 gctttgcaaa gaatggtaat gtattcccta ttagcccctt gatagttttc tgaataggca
     3841 cttctaacaa ttttgattac ttctttttct tctaagggtt gatctaatcg attattaaac
     3901 tcaaacatat tatattcgca cgtttcgatt gaatagcctg aactaaagta ggctaaagag
     3961 agggtaaaca tgacgttatt acgccctatt aaaccctttt ctcctgaaaa tttcgtttcg
     4021 tgcaataaga gattaaacca gggttcatct acttgttttt tgccttctgt accgcttaaa
     4081 accgttagac ttgaacgagt aaagccctta ttatctgttt gtttgaaaga ccaatcttgc
     4141 cattctttga aagaataacg gtaattagga tcaaaaaatt ctacattgtc cgttcttggt
     4201 atgcgagcaa taccaaaatg attacacgtt agatcaactg gcaaagactt tccaaaatat
     4261 tctcggatat tttgcgaaat tattttggct gctttgacag atttaaattc tgattttgaa
     4321 gtcacataga ctggcgtttc taaaacaaaa tatgcttgat aacctttatc agatttgata
     4381 atcatagtag gcataaaacc taaatcaata gcggttgtta aaatatcgct tgctgaaata
     4441 gtttcttttg ccgtgtgaat atcaaaatca ataaagaagg tattgatttg tcttaaattg
     4501 ttttcagaat gtcctttcgt gtatgaacgg ttttcgtctg catacgttcc ataacgataa
     4561 acgttgggtg tccaatgtgt aaatgtatct tgattttctt gaatcgcttc ctcggaagtc
     4621 agaacaacac cacgaccgcc aatcatgctt gattttgagc gatacgcaaa aatagcccct
     4681 ttgcttttac ctggcttggt agtgattgag cgaattttac tatttttaaa tttgtacttt
     4741 aacaagccgt catgaagcac agtttctaca acaaaaggga tattcattca gctgttctcc
     4801 tttcctataa atcctataaa ataggttgtt taattaactt ggtttgcttt ttcattcaac
     4861 tgtttcaata ttgcatgttt tgaaaaagat ttttttcctt tataagtcaa tttttttcca
     4921 ctaatcgaat aaattatttt gttattttct attaacttat atatataatc ttccccctcc
     4981 gaagaaaaat acttatctga ttttgtttct aagtagatat ttctcttttc taactctttc
     5041 ttaaacgttt ctagtgtata gatatttgct aattttctta tctccaataa actatttttt
     5101 atataagttt tacattcatc atgattcata caaactccac cttctataaa tgaatacaaa
     5161 aaaagcaatc aaacgatttc cgattgattg cttaacaatt cttaaattca gtagcttaga
     5221 tacttgaaaa ctctctgatt tccctatata atgatagtac ggttatatac cgtcttcaaa
     5281 caaagttaat taaataactt cttacgaggg aagagttcat ctgactaact gataagcgtt
     5341 ggtttggcaa tcttatcggg ctatgcattt ataaaatgtc gtcaaacatt ttataaatgt
     5401 gtcatggctc ttttttcgtt tctattcagt tcgttgtttc gttatatcta gtataccgct
     5461 tttaaaaaaa ataagcaacg atttcgtgca ttattcacac gaagtcattg cttttttctt
     5521 cttccatttc taaatccaat gttacttgtt ctgattctgt ttctggttct ggttctgttg
     5581 gctcatttgg gattaaatcc actactagcg ttgagttagt taactttgca atttgttcta
     5641 gtgtttttat ggttggatct gattttcctg attctattcg tgaataattt gatctactca
     5701 tttctaattc ttgggagaat ttttcttttg atttggtacc tctcatcttt tttaagcttt
     5761 ctctaaatgc tagttccaag tttctccccc ctctttttgt gtaataaaat tcacgcttta
     5821 tttaaattat agcttttaat atcttaataa acaagtgatt aatcacttgt gatttgtgat
     5881 tattccaaaa gtaggtaatt tcaccaacct ttttcaaaag tttttatcga cgattattgg
     5941 actggatcgt gtttcctaaa gcgattctaa gcgcatgttt aattttaaag tgggtaattc
     6001 tctttaaaac gtcctacaaa gattcaaaaa aagcatggct gctagtgggg gtaccgtcag
     6061 gaaaatgcgg atttacaacg ttaaggaaat ttattaaaga gaccaattcc tcagcactaa
     6121 aattgataat tgggctttct ttgttactat agtgttaggt aagcttttta ctttatcacc
     6181 ggttttcccc tcacttcaca cctacgggcg agtttcccca catcccgttt tccatctaat
     6241 ccaattcatt caatatcttt atgtgtatga aagatcagat tgcttgattt agacatctta
     6301 cacgtaattt attcttaaat gggtacggta aacgagcaat aatacgagca aaagtatacc
     6361 tacaaaaata ataactctgt ctaatttttt ttgattcata tgcttattgc ttaaaccata
     6421 taatttcagt ccaagaaagc ctccaacagt atttgtaatt acatctgtta tgtctgtcgc
     6481 tccaatagcg aagataaatt gaattatttc aaaagtaaga cttaaaacca gtacaaaagc
     6541 aaacttaggt aaaaatccga tttctttaaa attgacattc aaaagcaagc caaatggaat
     6601 aaagattata acattatcta tcatctctct gaaattccca gtagcagtaa atggagtcaa
     6661 gttaagactt ctttgatgat aattaaatac tgataaaata ttgtattgta atttgaataa
     6721 cactaaccag attagtgtca ctaaatataa agctagcaat cctctagata atatttttcc
     6781 caatataatt tcctctaatt cttttgttta agttgtatca ttagtatagc aatgtcctag
     6841 ttaacgtggg ttttaaatta aaaagctaac gttaccccaa aagtgctagt acaataatgc
     6901 tgttcattaa tttcgttggt ttcattccgc catccttacc tccttgaatt agtatgtgtt
     6961 gatccgggaa acactgtcac aattacaccg tctatattgt ttcctgatat ctcataacga
     7021 agattagtcg gcacatgaat ggtggtattt ttagtaacag gataataaaa gatctcatat
     7081 ttttccccat ttacactaac agaaatggtt ttttcttctt ttaggtaatc catatattcc
     7141 tcgagaacga aattcttttc tttcataatc gcactatgtg gtaaaccaac atagcgaata
     7201 tgccatggtt catattgaat tcctgttaac tctgttttgt cctctggata acgtaaaatg
     7261 aacccgtatt tccaagcatt ttcttctatc cactttcctt caggggctcg ttccattttc
     7321 gtcaagcttg atcctacatc tagtgataaa cctgaattat gctcactata acctgctggt
     7381 aaggcatact cagcccccat ttcttggtaa agcacacttt gctcatcaaa gtctcgatag
     7441 ccactattaa taataaaatg actaacgcca ccctttacag catcattgac catctctgaa
     7501 aatttttgtg ctatttcttt tgacatataa atattactat caagcaaccc gtatccattt
     7561 attaattcgt catgtttaga taaattcacg atatctgact tcacactttc ttggcgaaca
     7621 ggatatttac tattgattaa tagcagattt ccttgataaa cctgttcttc tgtaattgtt
     7681 ttctcttggg tattttcaga agtcccacta ttttctaaat gttctttggg attttgatca
     7741 taattttgaa attcgacttt ttcctgagaa aacagtgctt cattaacgta gtcataacct
     7801 aagtatatta agaataacaa taacaataaa aaaaacaact tcttcatttt cagtctcctc
     7861 cttaactata tagttggaag tatgaaatat ttctagaatc ttccccttaa acggcacaat
     7921 ctcgtttgag attgtgccgt tttgccccgt aaattacagt caattatttc gccctactgg
     7981 atttactatt atcaccaatg tagatgaatt ttcataagtg atacccatgc tgaatttaac
     8041 gaggctgaac tgcctgttga caatg
//
LOCUS       DAZKLM010000108         8025 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3764
            SAMEA115706913-rid22582563.denovo.113, whole genome shotgun
            sequence.
ACCESSION   DAZKLM010000108 DAZKLM010000000
VERSION     DAZKLM010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706913
            Sequence Read Archive: ERR13196647
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8025)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8025)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287823.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287823.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,004
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,849
            CDSs (with protein)               :: 2,849
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 41 of 96
            Pseudo Genes (incomplete)         :: 52 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 15 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8025
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706913-rid22582563.denovo.113"
                     /isolate="VRE3764"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(46..894)
                     /locus_tag="ACG842_002419"
     CDS             complement(46..894)
                     /locus_tag="ACG842_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFQ0248920.1"
                     /translation="MSVISRIESMMEDYSSAEKKLANYIIGNIEKVPTMTANELAEAS
                     GLSAPTVVRFSKKIGFQSLTDFKITISTELQTGIDEGFSDIEANESFYSIKNKLGNNA
                     QVAIKETVDILEEETIQKVVECLESAETVFLYGVGASSLVVEDILQKWSRVGKPIIFE
                     KDIHVLLPQLVSNEKKKVLWLVSNSGRSADVVALAELAKSMNIEIIALTQFGNNPLSK
                     IADVLVQTSRPKEITNRSAATNSLLAQFATIDIIFYFYMAKNEKLSEKVKKTREAIQE
                     YFMDSK"
     gene            complement(1118..3058)
                     /gene="thrS"
                     /locus_tag="ACG842_002420"
     CDS             complement(1118..3058)
                     /gene="thrS"
                     /locus_tag="ACG842_002420"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HFQ0248921.1"
                     /translation="MIKITFPDGAVKEFESGTTTLAIAESISKSLAKKALAGKVNGKL
                     VDLTRPIEEDASIEIITPDHEDALGLVRHSAAHLMAQAMRRLYPNIHFGVGPAIDSGF
                     YYDTDNGQNQVTAEDLPAIEAEMMKIVKENLPIERRVLSKQEALEIFASDPYKVELIS
                     ELPEEEVITAYQQGEFIDLCRGPHVPSTGRIQVFKLLSVAGAYWRGNSNNQMMQRVYG
                     TAFFDKKALKEYIRLREEAKERDHRKLGKELDLFMVSPEVGSGLPFWLPKGATIRRTI
                     ERYIVDKEVSLGYQHVYTPIMGDVELYKTSGHWDHYQEDMFPPMDMGDGEMLVLRPMN
                     CPHHMMVYKNTIHSYRELPIRIAELGMMHRYEKSGALSGLQRVREMTLNDGHTFVRPD
                     QIKDEFKRTLELMVAVYADFNITDYRFRLSYRDPNNTDKYFDDDAMWEKAQAMLKAAM
                     DELELDYFEAEGEAAFYGPKLDVQVKTALGTEETLSTIQLDFLLPERFDLTYVGEDGE
                     NTHRPVVIHRGIVSTMERFVAYLTEVYKGAFPTWLAPIQATIIPVSVDAHGDYAYEIK
                     ERLQMKGLRVEVDDRNEKMGYKIRASQTQKIPYQLVVGDKELEDATVNVRRYGSKETA
                     VEDLNIFIDAMEAEVKNYSREN"
     gene            complement(3395..3574)
                     /locus_tag="ACG842_002421"
     CDS             complement(3395..3574)
                     /locus_tag="ACG842_002421"
                     /EC_number="5.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016862 - intramolecular oxidoreductase
                     activity, interconverting keto- and enol-groups [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxymuconate tautomerase"
                     /protein_id="HFQ0248922.1"
                     /translation="MPFVHVELVEGRSPEQLENMMKDITEAVHKNTQAPKEHIHVIIN
                     EMKKGTYGVNGEWKK"
     gene            complement(3621..5444)
                     /locus_tag="ACG842_002422"
     CDS             complement(3621..5444)
                     /locus_tag="ACG842_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFQ0248923.1"
                     /translation="MTHFKKFLLTLLIGGIALLSEFVFHQPALAFAIVAFTGGILAFL
                     MFLEMIKTLRSGRYGVDVLAITAILATLAVKEYWASLMILIMLTGGDSLEDYANKRAG
                     KELQSLLDNTPRIAHRFKEGKLQDIVIDEINIDDQLLVKPGELVPADGRVTKGESSVD
                     ESSLTGESRPETKKPGDQIMSGSLNGDGSIQMIAEKRAADSQYQMIIKLVEESKEKPA
                     RFVRLADRYAVPFTLIAYLIGGISWWFSKDPLRFAEVLVVASPCPLILAAPIALVAGM
                     SRSSKNGIVVKTGTTIEKLARTKTIAFDKTGTLTKGILEVEAIQPVNGIKKEELLLYA
                     ASAEQESSHILARSLVNNVKNSQLYPVSQLKEVTGQGIQAVVNNHEVKIGRASFVSAE
                     EIETSNTAIYVTIDRSFAGTILFSDQIRPEAAKTIKELRKLDVKNLMMITGDGSAIAQ
                     TIAEKVQLDSVHARSLPQDKLTILESIPKDQRPVAMVGDGVNDAPALAAADIGIAMGA
                     HGSTAASESADAVILKDDLLRVAKAVVISRDTMRIAKQSVLIGIFICIFLMLIASTGL
                     IPALFGAVLQEVVDTVSILSALRAKKDPAIFPDSVPKTNIV"
     gene            5722..8001
                     /gene="helD"
                     /locus_tag="ACG842_002423"
     CDS             5722..8001
                     /gene="helD"
                     /locus_tag="ACG842_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase recycling motor HelD"
                     /protein_id="HFQ0248924.1"
                     /translation="MNEKQLEQTHVDETIELIQIEQNMLDTKQQELTQEMNHSTKDTA
                     NHKIRAGSNEAFYESAVEYRQHEQELLLKYHTLEAQQKRLQTLSVMKDSPYFARIDFK
                     EDRETETLYLGIASLRDLAEETIVIDWRAPIANLYYEGEIGPAFYETETDRIDVDLQL
                     KRQFKIQEGRILSMVDTSEVINDDFLLEILDESSSNHMKNIVSTIQKAQNAIIRDTTS
                     KVMLIEGIAGSGKTSALLQRIAFLLYHNRKWLEAENVLLFSPNHLFSDYISTVLPSLG
                     ESGVPTQTFKNYLVQLLPEFSLLDEQQQESGFLLGEKDPIQVMKSGLTLVDQIDRYIQ
                     GITNYGPLFRDMKINGRTILSKESIRQWYKETNEQLPLHHRLSLLQTKLLKKLGGLQK
                     DETRQQWVKDLAEEQLQELYATDPNLEYTEQKERSLRKKLANQIVRKRFRRIHRGIMN
                     YQFVNITKQYLHFLQTVPLSLLTRHKISETRWQEHLTTVRTHLRQRELRQEDAVLFFL
                     LMRRTYPVTVTHKARYIFIDEMQDFAPSQVALLQALFPKANLTFCGDLNQNVFGNETI
                     VGSLEDLFVDREVTRFQLTTSYRSTKEITDFANHFIVKEHQVETTARKGDLPLLIQGT
                     TDDIKLNWLADTIRTTQGKARYWRTAIIAKTQEECEALYAQLPDELKQQVQLILNESD
                     FMKRSIILLPAFLAKGLEFDRVFLWDVDAEHFSSQQDKLILYTMCTRAMHELTLFTSN
                     NESPLLNNMDISSYEKKVL"
BASE COUNT         2353 a         1707 c         1451 g         2514 t
ORIGIN      
        1 acgttttgtt attctaaaaa acttatataa aaaatagtaa ggtttttatt tcgaatccat
       61 aaaatattct tggattgctt ctcgtgtttt tttcactttt tcgctaagct tttcgttttt
      121 agccatatag aaataaaaaa tgatatcgat cgtagcaaat tgggccaaaa gtgaattagt
      181 tgccgcactt cgattcgtga tttcttttgg tctagatgtt tgtacgagta catcggctat
      241 cttgctaagc gggttatttc caaattgcgt caacgcaatg atttcgatat tcatgctttt
      301 cgccagctcg gctaatgcga ctacatctgc acttctacct gagtttgaaa caagccaaag
      361 cactttcttt ttctcattgc tgacaagttg cgggagcaag acgtggatat ctttttcaaa
      421 aatgattggc ttaccgacac gagaccattt ttgcagaata tcttctacta ctaaagaaga
      481 ggcgccaacc ccatataaaa aaacagtctc tgccgattcc aaacactcta cgactttttg
      541 gatcgtttct tcttctagaa tatctactgt ttcttttatc gctacttgtg cattgttacc
      601 aagtttgttt ttgatagagt aaaaggactc atttgcttct atatcactaa aaccttcatc
      661 gattccagtc tgtagttctg ttgaaatcgt gattttgaag tccgtcagac tttgaaaacc
      721 gatttttttg gaaaaacgta caactgtcgg cgcactcaac ccggaagctt ctgctaattc
      781 attagctgtc attgtaggga ccttctcaat attaccgata atgtagttgg ccaacttttt
      841 ttctgctgaa gaataatctt ccatcatact ttcaatacga ctaattacgc tcataagttc
      901 ctccaccctt ttaatgatag tctatgtaag tataccatat cagaccctta ttcacggtcg
      961 tatactggat aaaaaacaaa aaaacaatcc gcccaacaga atatcttatc ccatcaatcc
     1021 cacagattct attgagcatc tgtgtctttg aggaatacct gttatctatt gttcggattg
     1081 ctttctatct actacttttt tacaggaaat ttacctatta gttttctctg ctgtaatttt
     1141 tgacttctgc ttccatagca tcaataaaga tattcaaatc ttctacagct gtttctttac
     1201 ttccataacg acgtacgttc actgttgcat cttccaattc cttgtctcca acaactaatt
     1261 ggtaagggat cttttgtgtt tgagaggcac gtatcttata gcccattttt tcattacgat
     1321 catcaacttc tacacgcaac cccttcattt gcaaacgttc tttgatttca taagcataat
     1381 cgccatgtgc atcaacagaa acagggatga ttgttgcttg tataggtgcc aaccatgttg
     1441 gaaaagcgcc tttgtatact tctgttaaat aagcgacaaa acgttccatt gtagaaacaa
     1501 ttccgcggtg gataactact ggacgatgcg tattttcccc gtcttctcca acatatgtca
     1561 aatcaaagcg ctctggcaat aagaagtcta attgtatagt tgataatgtt tcttctgtac
     1621 caagagctgt tttgacttga acatctagtt ttggtccgta aaaggctgcc tcgccttctg
     1681 cttcaaagta atctagttct aattcatcca tagcggcttt cagcattgct tgggcttttt
     1741 cccacattgc gtcatcatca aaatatttat ctgtattgtt tggatcgcga tagctcaagc
     1801 ggaaacgata atctgtaata ttgaagtcag catatactgc caccatcaat tcaagcgtac
     1861 gtttaaattc atctttgatt tgatcaggac gaacaaatgt atgaccatca ttcaacgtca
     1921 tttctcgtac acgttgtaaa cctgacaaag cacctgattt ttcatagcga tgcatcattc
     1981 ctaattcagc gatacgaatt ggtaattcac gataagaatg gatcgtgttt ttgtagacca
     2041 tcatgtgatg agggcagttc attgggcgaa gaacaagcat ttcgccatct cccatatcca
     2101 ttggcgggaa catgtcttct tgataatgat cccagtgtcc agatgttttg tacaactcaa
     2161 catctcccat tattggtgta tatacatgtt ggtaacctag actaacttct ttgtctacga
     2221 tataacgttc gatcgtgcgt cggattgttg ccccttttgg aagccagaat ggcaaaccag
     2281 aacctacttc tggggaaacc atgaacaagt caagttcttt tcctagttta cgatggtctc
     2341 tttcttttgc ttcttcacgg agacggatat attctttcaa agctttcttg tcaaaaaagg
     2401 ctgtcccgta tacacgttgc atcatttgat tgtttgagtt tccacgccaa taagcacctg
     2461 ccactgataa taatttaaat acttgtatac ggccagtgga tggaacatgt ggtccacgac
     2521 acaaatcgat gaattcgcct tgttgataag cagtaatcac ttcttcttct ggcaattcac
     2581 taatcagttc tactttgtac ggatcacttg caaaaatttc taatgcttcc tgttttgaaa
     2641 gaacacggcg ttcgatcggc agattttctt tgacgatttt catcatttct gcttcgattg
     2701 ccggcaaatc ttctgcagtt acttggtttt gtccattatc tgtatcgtag taaaaccctg
     2761 aatcgatagc tggtccaacg ccaaagtgga tatttggata gaggcgacgc atggcttggg
     2821 ccatcaaatg agctgctgaa tgacggacta agccaagtgc atcttcatgg tctggtgtga
     2881 tgatctcaat acttgcatct tcctcgattg gacgagtcaa atcaacgagt tttccgttca
     2941 ctttaccagc taaagctttt tttgctaagc tttttgaaat gctttcagca attgccaatg
     3001 ttgttgttcc agactcaaat tctttgactg caccatctgg aaaagtaatt tttatcataa
     3061 tagtttcctc cttgaaatgt tttgattgat cagatcatca agctgaaaaa caaaaaaatc
     3121 ctagcatctc ttgaaaagag atactaggac gaaaagttcg tggttccacc taatgttcaa
     3181 acgcaaatgc gttcctcaag cgttgtaacg gtccgaccgc ccttgcttca acaagggttt
     3241 cgaaagtggt catcctttgg ttttctcctg gaaaagtttc agcctagctt ttcctctctt
     3301 atcgaagact ctcaaattca gttggctttc tcatcaatca attatgtgtt tatttaacca
     3361 ctaataaata gaaaaatcaa gtgggaaact aaacttattt tttccattcc ccatttacac
     3421 cgtatgttcc ttttttcatc tcattgatga tgacatggat gtgttctttt ggtgcctgcg
     3481 tgtttttatg aacagcttcc gtgatatctt tcatcatatt ttctaattgt tccggactgc
     3541 gaccttctac taattcgaca tgtacgaatg gcataatgct ctctcctctt tctgatcgtt
     3601 cttttttcaa ctcgattcta tcatactatg tttgtcttgg gtactgaatc aggaaaaatg
     3661 gctggatctt tttttgctct caaggcactc aaaatagaga ccgtatccac gacttcttgc
     3721 aaaacagctc cgaataatgc ggggattagt cccgtacttg caattaacat caaaaaaata
     3781 caaataaaga tgccaatcag tacagactgt ttcgctattc tcatcgtatc tcttgaaatc
     3841 actactgctt ttgctacacg caagagatca tctttcaaga tgaccgcatc tgcactttca
     3901 ctagcagctg tagaaccatg agcccccatt gcaataccga tatctgcagc agcaagtgct
     3961 ggtgcgtcat tcacgccgtc tcctaccatc gcaacaggtc tttgatcttt agggatactt
     4021 tctaaaatgg tcagtttatc ttgaggcaag ctacgggcat gtacagaatc taattggact
     4081 ttttctgcaa tcgtttgagc aatggcagat ccatccccgg tgatcatcat caaatttttc
     4141 acatcaagtt ttcttagctc cttgattgtc tttgctgctt ctgggcgaat ctgatccgaa
     4201 aataggatcg ttcctgcaaa agatcgatca attgttacat agattgctgt attggacgtt
     4261 tctatttctt cagctgaaac aaaagaggca cgtccaatct ttacttcatg attattgact
     4321 actgcttgga ttccttgacc ggtcacttct ttcagttgac taacaggata cagttggctg
     4381 tttttcacgt tgttcactaa cgaacgagct aagatatggc tagattcctg ttctgcacta
     4441 gctgcataca gaagtagctc ttcttttttt attccattga ctggctgaat cgcctctact
     4501 tccaatatcc ctttggtaag cgttcctgtt ttatcaaaag caatcgtttt tgtacgtgcg
     4561 agcttttcaa tcgttgttcc agtttttaca acgatcccgt ttttacttga acggctcatt
     4621 cctgcaacta aagcaatggg cgcagctaga atcagtggac aaggtgatgc cactaccagt
     4681 acctcagcaa atcgtaaagg gtcttttgag aaccaccaag aaataccacc gatcaaataa
     4741 gcaatcaacg tgaaagggac tgcataacga tcagctaaac ggacaaatcg ggcaggcttt
     4801 tcttttgatt cttcgactag tttaataatc atttgatact ggctgtctgc ggcacgtttt
     4861 tcagcgatca tttggatcga tccgtctcca tttaaagacc ctgacataat ctgatctcca
     4921 ggtttcttgg tttccgggcg ggactcacct gtcaaagaag attcatcgac cgaagattct
     4981 cctttggtca ctcgaccatc tgcgggtaca agttcgccag gcttgactag taactggtca
     5041 tcaatattta tttcatcgat tactatatcc tgcaattttc cttctttgaa tcgatgggca
     5101 attcttgggg tgttatctaa caaagactgc agttctttac ccgctcgttt attcgcgtaa
     5161 tcctctagac tatctccgcc cgtaagcatg atcaatatca tcaaactagc ccagtattct
     5221 ttgacggcta atgttgcaag gattgcggta atcgctaata catctacgcc atatcttcct
     5281 gaacgtaacg tcttgatcat ttccaaaaac atcaagaagg cgagaatccc tccagtaaat
     5341 gcgacaatcg caaatgcaag ggccggctgg tgaaaaacaa attcacttaa taaagctatc
     5401 ccaccaataa gcaaagtaag taaaaatttt ttgaaatgtg tcattgtttt ttctcctctt
     5461 ttatgtgact tagagaggtt agtaataaat ttccagctgc ctctttcatc ttaagcatag
     5521 tccttgacta gcacttttta aaacaatagg ccttaattga aaacaactat cactatcagc
     5581 gtttccaaaa ttcatcagtt ctacaaacaa aaatattcag caaggattac tttttcgatt
     5641 catcagttaa aaagtacctt ttttcgaaaa actatgctat acttttaatt cgttatttac
     5701 ggcggaagga gcaaacatgc gatgaatgag aaacaactag aacaaacgca cgtggatgaa
     5761 acgatcgaac taatccagat cgaacaaaat atgctggata caaaacaaca agaattgact
     5821 caggaaatga atcattcgac aaaagacaca gctaatcaca agattcgagc tggttccaat
     5881 gaagcttttt atgaatctgc tgttgaatat cgccaacatg agcaggaatt gcttttgaaa
     5941 taccatactc ttgaagcaca gcaaaaacgc ctgcaaacgt tgtctgtcat gaaagatagt
     6001 ccttattttg cccgtatcga ctttaaagaa gaccgtgaaa cagaaacatt atatctaggt
     6061 atcgcttctt taagagatct ggcagaagaa acaatcgtta ttgactggcg cgcaccaatt
     6121 gccaatcttt attatgaagg tgagattggt cccgcatttt atgaaactga aacagataga
     6181 atagatgtcg atctgcagtt gaaacgacaa tttaaaatcc aagaaggacg tattttatct
     6241 atggtagata cgtcggaagt aatcaatgat gactttttgc tagagatatt ggatgaatca
     6301 tcaagcaacc atatgaaaaa catcgtctct acgatacaaa aagcacaaaa tgcaatcatt
     6361 cgggacacaa caagtaaagt aatgctgatt gaaggaatcg ccggaagtgg aaagacatcc
     6421 gctttgcttc aacgtattgc ttttctgttg tatcataatc gcaagtggct cgaggctgaa
     6481 aacgttcttc tattttcacc caaccatttg ttttctgatt acatctcgac tgtattgcct
     6541 tcattaggtg aaagcggcgt gcctacacag acattcaaaa attatttggt gcagctgctg
     6601 ccagagtttt ctttactaga tgaacagcaa caagaaagtg gttttttgct aggggaaaaa
     6661 gatccgatac aagtgatgaa aagcggattg accttagtcg atcagattga tcgctatatc
     6721 caaggcatca caaactacgg tcccttgttc cgtgatatga aaataaatgg ccgtacgatc
     6781 ctatcaaagg aatcgattcg ccaatggtat aaagaaacaa atgaacaatt acctttacat
     6841 catagacttt ctctattaca aacgaagctc ctgaaaaaat taggcggtct gcaaaaagac
     6901 gaaacacgtc agcaatgggt caaagatctg gcggaagaac agctccaaga actatacgca
     6961 acagatccaa atcttgaata tacagaacaa aaggagcgtt cattacgtaa aaaactggcc
     7021 aaccaaatcg tacgtaaaag attccgtcga atccatcgag ggatcatgaa ttatcaattt
     7081 gtcaatataa cgaaacaata tctccacttt ctacaaactg tgccactttc tcttctaaca
     7141 cgacataaaa tcagcgagac caggtggcag gaacatttga caactgttcg tacacacttg
     7201 cgtcaaagag aattgcgtca agaggatgct gtactattct ttttactgat gcgccgaaca
     7261 tatccagtga cggttacaca caaagctcgc tatatcttta ttgatgaaat gcaggatttc
     7321 gcaccatcac aagtggcatt gctgcaagcc ttgttcccaa aagcaaattt gactttctgt
     7381 ggagatctaa atcaaaatgt ctttggaaat gaaacgatcg ttggatcatt ggaagacctt
     7441 ttcgtggacc gtgaagtcac tcgtttccaa ttaacgacaa gctatcgctc cacaaaagaa
     7501 atcactgatt ttgctaatca ttttattgtg aaagaacatc aagtagaaac aacagcaaga
     7561 aaaggagacc ttcctttgct catccaagga acaacagatg atatcaaatt gaattggtta
     7621 gcagatacca ttcgaacgac tcagggaaaa gcccggtatt ggcgtacagc aatcatagct
     7681 aaaacacaag aagaatgcga agctctttat gctcaactgc cagacgagtt gaaacaacaa
     7741 gtccagctga ttttaaatga gagtgacttt atgaagcgtt ccattatttt acttccagcc
     7801 tttttagcaa aaggtctgga gtttgaccga gttttcttgt gggatgtaga tgcagaacat
     7861 ttttcttctc agcaagacaa attgatcctt tacaccatgt gtacacgtgc gatgcatgaa
     7921 ttgacccttt ttacctccaa taatgaaagt cctttgttaa acaatatgga tatttcatct
     7981 tatgagaaaa aagtactgta agtaaaaaga caactgctat catta
//
LOCUS       DAZKLM010000109         7934 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3764
            SAMEA115706913-rid22582563.denovo.114, whole genome shotgun
            sequence.
ACCESSION   DAZKLM010000109 DAZKLM010000000
VERSION     DAZKLM010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706913
            Sequence Read Archive: ERR13196647
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7934)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7934)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287823.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287823.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,004
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,849
            CDSs (with protein)               :: 2,849
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 41 of 96
            Pseudo Genes (incomplete)         :: 52 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 15 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7934
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706913-rid22582563.denovo.114"
                     /isolate="VRE3764"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            181..882
                     /locus_tag="ACG842_002424"
     CDS             181..882
                     /locus_tag="ACG842_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HFQ0248925.1"
                     /translation="MRVNKIDFHFSKLRYQPDFEHFSDEEFQQIKEHTVLRSYKKGQI
                     LFDDGDPRQRLYILTKGLVRLERYDESATYYYYDYVNTNNLFPMSGMFGEETYTYTAQ
                     AMTDIETFYLPVSYYEKLARKNPEQLIYMIQKISKVVEIHELRIQIGLTSSAFDRVKQ
                     NLFILKEELGVPTKDGHITIPYPITLKELAVNSGTTRETAGQVIKQLRDSGLLDYDKK
                     RFTFCKTATTPAMRE"
     gene            complement(1491..2507)
                     /gene="tsaD"
                     /locus_tag="ACG842_002425"
     CDS             complement(1491..2507)
                     /gene="tsaD"
                     /locus_tag="ACG842_002425"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HFQ0248926.1"
                     /translation="MNEELILAIESSCDETSVAVVRNGTEILSNIVASQINSHKRFGG
                     VVPEVASRHHVEQITLCLEDALVEAGVSAEDLSAVAVTYGPGLVGSLLIGISAAKAFA
                     WAHQLPLIPVNHMAGHIYAARLVKPFQFPLMALLVSGGHTELVYMQEDGSYEIIGETR
                     DDAAGEAYDKVGRVLGLSYPSGKEIDQLAHQGKDNYHFPRAMIHEDNYDFSFSGLKSA
                     FINLVHNAQQRGEDLDKNDLAASFQASVIDVLITKTLRACQNYPVKQLIIAGGVAANQ
                     GLREGLQDALATKLPEVELVIPPLRLCGDNAAMIGAAAHVEMQKRNFASYQLNADPSL
                     VLSE"
     gene            complement(2504..2968)
                     /gene="rimI"
                     /locus_tag="ACG842_002426"
     CDS             complement(2504..2968)
                     /gene="rimI"
                     /locus_tag="ACG842_002426"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HFQ0248927.1"
                     /translation="MICRKKDFDEAYLAECVWMISENAYPHGSPWTKMQFLADIQQPH
                     SDYLLLAEDNQICGFIGYSKVIDEVEITNIAVAVSEQGKGHARHLLQLLITEQIKDSL
                     HVFLEVRLSNEAARKLYESEKFRILGKRKSYYRNPTEDAIIMSRKLKTETMK"
     gene            complement(2975..3517)
                     /gene="rimI"
                     /locus_tag="ACG842_002427"
     CDS             complement(2975..3517)
                     /gene="rimI"
                     /locus_tag="ACG842_002427"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HFQ0248928.1"
                     /translation="MLKKFRAYARSFFQDKMIYTEKTVEQIDRRYVLRELTTEDIKEL
                     LDVEREVYNGELPWTKSAFISELKSPLPHLYLGIFDNGRMIGFIGSRAFGLDCHITNI
                     AVRPAYQRKRIGSLLIDEIENFAIMNRCETMSLEVRMSNQDAQRLYRKLGFQATTIKK
                     RYYTETKEDALDMIKLLENE"
     gene            complement(3501..4223)
                     /gene="tsaB"
                     /locus_tag="ACG842_002428"
     CDS             complement(3501..4223)
                     /gene="tsaB"
                     /locus_tag="ACG842_002428"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HFQ0248929.1"
                     /translation="MITLGIDTANQTLAIGVVEDEQILGQIQTNIKRNHSVTLMPAID
                     QLFADLQISPKDIDRIAVSDGPGSYTGLRIGVTTAKTIAYTLDKELVGVSSLKTIAAN
                     CIGVDNIIVPMFDARRKNVYAGAYRWKENRLETVLEDVHISASDLFTQLQKLNEAIYF
                     VGSDCHKFFSEIKEILPSAMINTVPAWDIPSGVTVAQLGALVEPVAEKQAFLPRYLKR
                     VEAEEKWLETHTPGDESYVEKI"
     gene            complement(4414..5394)
                     /locus_tag="ACG842_002429"
     CDS             complement(4414..5394)
                     /locus_tag="ACG842_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFQ0248930.1"
                     /translation="MATIKDIAQLAGVSPATVSRVLNYDPDLSVGLATKQKIFEAAER
                     LNYTKHRKNAANTKQTLRLIQWYDDTEELEDLYYLSIRLGIEKKAEEANVTLLKETWD
                     SISDLPADGTIALGKFDEAQLLEMKGSQNALLLVDSDGTSQGIDSLVVDFKSSVQKVI
                     EHFVSHKAQHIAMLAGTEYTKKNHQRLKDPRIMAFQQVLAEKGYDEGPIIEADFSVES
                     GYQAVKQYLKTNPHVPDALFAANDALAIGALKAIQEKGLVVPEDISVMGFNDVSVAKY
                     VTPSLSTVKVYTEWMGELAVETILQLSNSKAPVSRKIIVETELIIRESTK"
     gene            complement(5418..6902)
                     /gene="galT"
                     /locus_tag="ACG842_002430"
     CDS             complement(5418..6902)
                     /gene="galT"
                     /locus_tag="ACG842_002430"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0017103 - UTP:galactose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019388 - galactose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HFQ0248931.1"
                     /translation="MSISQTICDFATLAIEAGGWMEMDRLYLQNRLLGMVGEDALESI
                     EVRPVKEESVDLLDQLVAKARENGVLSELPAEQEIFEAQLMDFLTPPPSVVNAFFAQH
                     YSKSPEEATEYFFNLSKKNDYIKTRAIAKNIKFPAESEYGELEITINLSKPEKDPKQI
                     AAEREAVKVDYPTCMLCMENEGYRGRLNYPARTNHRIIRMNLDGESWGFQYSPYAYYN
                     EHSIILSEQHRPMKITKETFARLLRITEVLPHYFVGSNADLPIVGGSILSHDHYQAGR
                     HEFPMAKAPIEKLIKLEDYPQVIAGVVKWPMSVIRLQSTEKNLLIEAANSVLEKWKAY
                     SDETVSIRAFSADGTPHHTITPIARRRDNYFELDLVLRDNNVSEEHPDGIFHPHKDVQ
                     HIKKENIGLIEVMGLAVLPPRLKPELVEVEHFLLGEENQMAEYHRPWAEQLKKEHPDL
                     TKDTVTDVVQKSVGQVFARVLEDAGVFKRDEKGQAAFERFTAIL"
     gene            complement(6914..7903)
                     /gene="galE"
                     /locus_tag="ACG842_002431"
     CDS             complement(6914..7903)
                     /gene="galE"
                     /locus_tag="ACG842_002431"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HFQ0248932.1"
                     /translation="MTILVLGGAGYIGSHAVDQLVQKGYQVAVVDNLLTGHKQAVHPD
                     AHFYEGDIRDKEFLRSVFEKEPIEGVIHFAASSLVGESVEKPLMYFNNNVYGMQILLE
                     VMHEFNVNKIVFSSTAATYGEPKESPITEDTPANPKNPYGESKLMMEKMMKWCDQAYG
                     MRYVALRYFNVAGAKADASIGEDHTPETHLVPIILQVALGQRKALAVYGDDYDTPDGT
                     CIRDYVQVEDLIAAHILALEYLKEGNESNFFNLGSSKGYSVKEMLEAAREVTGKEIPA
                     EIAPRRAGDPSRLVASSEKAREILGWKPEYTDIKAIIKTAWDWHVSHPNGYEE"
BASE COUNT         2232 a         1707 c         1397 g         2598 t
ORIGIN      
        1 tatacacaaa tcattcgctt caaaacaaat tattcaatac cattgtatat tttttacata
       61 ataacaaatt atatttgtaa agtattaaaa cttgtttcta atgttggtat gttaaaactt
      121 tacaaattct ttaaaaaaag tgacaataaa tatagtgaac aataaggaga ggacttttta
      181 atgcgtgtta ataaaatcga ttttcacttt tcaaaactca gatatcaacc tgattttgaa
      241 catttctcgg atgaagagtt ccaacagatc aaggaacata ctgttcttcg ttcttataaa
      301 aaaggacaga tccttttcga cgatggtgat cccagacaac ggttgtatat cttgaccaaa
      361 ggactcgttc gtttagaacg ttatgatgaa agtgcaactt attactacta tgactatgtg
      421 aatacaaata acctttttcc tatgtcagga atgtttggag aagagaccta cacttataca
      481 gctcaagcga tgactgatat tgaaacattc taccttcctg tttcctacta tgaaaaatta
      541 gcacgaaaaa atcccgaaca gttgatctat atgatacaaa aaatttctaa agtagttgaa
      601 atacatgaat tgcgtatcca aatcggattg acctctagcg cctttgaccg ggtaaaacaa
      661 aatttattca ttttgaaaga agaattgggc gtaccaacaa aagatggtca tatcaccatt
      721 ccttatccga tcaccttaaa agaattagca gtgaatagcg gcacgacacg tgaaactgct
      781 ggacaagtaa tcaaacaact acgtgattcc gggctgttag attatgacaa aaagcgtttt
      841 actttctgta agacagctac tactccagct atgagagaat aacgcgaaca aaaataagca
      901 gtgaccctga taaatatcag ggtcactgct tattttcctc cctaaaactt ctgaaagatc
      961 cggcagtttt agatcactgg acaagactca aagattttct atgtgtgttt cagttatgta
     1021 atattcactg tttgcaaaaa atactgttag attaactaga tgaagcgtct tgtcctcatc
     1081 cgatctagat tattcgatat tcctcgacaa taaacgatta ctcagtgagg gcaatcagag
     1141 gaacggataa tgataaataa gctgtaaaaa atagcaaaat aacaataatg attaaaagta
     1201 tgtaaaaaat agggctttga caaaaacgtg tcacagccct atttcgatta aacggtgtgt
     1261 acctgtcaac tcttcggtca caagtcataa tgttcataaa catgaggagc tgttttttga
     1321 aattgttcct tgtgaatcag agttaaacga caggcttacg aactcttgat taaacggcgt
     1381 tcaaaagctt gcatacgcgg taataagaac agccaggtca aaaacatgag aagccgtttt
     1441 tcctgggctg ttcttattac ttgatgcagg gcagcttttt cacaacctct ttattcagat
     1501 aaaaccaagc ttggatcagc attcaactga tagctagcaa aatttctttt ttgcatttcc
     1561 acatgtgctg ctgctccaat cattgcagca ttatctccac aaagtcgaag agggggaatc
     1621 actagttcta cctcagggag tttagttgcc aaagcatcct gcaatccttc acgcaaccct
     1681 tgattggccg ctacacctcc agctataatc agttgcttga ctggataatt ttgacaagct
     1741 cgaagtgttt tagtaatcaa tacatcgatc acacttgctt gaaagctagc tgctaaatca
     1801 tttttgtcta gatcttctcc acgctgttgt gcattatgca ccaaattgat aaatgcactt
     1861 tttaatccgc taaaactgaa atcataatta tcttcatgga tcatcgctcg cggaaaatgg
     1921 taattatctt ttccctgatg agctagctga tcgatttctt ttccgcttgg gtaagaaaga
     1981 cctaatactc ttccaacttt gtcataagct tctcctgctg cgtcatctct tgtctcccca
     2041 atgatttcat atgatccatc ttcttgcata tagactaatt ctgtatgtcc tccgctaaca
     2101 agaagtgcca tcaatggaaa ctggaatggt ttaaccagac gggctgcata gatatgccct
     2161 gccatatggt ttacaggaat aagcggcaat tggtgtgccc aagcaaaagc cttagctgca
     2221 gaaataccaa tcagcaacga gccgacaagt cctggtccat aagtgacagc gactgccgat
     2281 aaatcttctg ccgacacacc agcttctacc agtgcatctt ccaagcataa ggtgatttgt
     2341 tctacgtgat ggcggctagc tacttcaggt actacgccac caaaacgttt atgactgttt
     2401 atttgagaag caacgatgtt agataagatc tctgttccat ttctaacgac cgctacactc
     2461 gtttcatcac aacttgattc aatcgccaga atcaattctt cattcatttc atcgtctccg
     2521 tttttaactt tctgctcata atgatcgcat cttccgtcgg attgcgataa tatgatttcc
     2581 gtttacctaa tatacgaaac ttttctgatt cataaagttt tcttgctgct tcattagata
     2641 gacgtacttc taaaaaaaca tgcagcgaat cttttatttg ttccgtaatc agtaattgta
     2701 acaaatgccg tgcatgccct tttccttgtt ctgaaactgc aacagcaata tttgtgatct
     2761 cgacttcatc tattactttc gagtatccaa taaagccaca gatctggtta tcttcagcta
     2821 aaagcagata atcagagtga ggctgttgga tgtctgccaa aaactgcatt ttcgtccaag
     2881 gtgaaccatg tggataagca ttttcgctaa tcatccatac gcattctgcc aagtacgctt
     2941 catcaaaatc tttttttctg caaatcaact aatctcactc attttctaaa agttttatca
     3001 tatccaacgc atcttctttt gtttcagtgt aatatctttt tttgatcgtt gttgcttgaa
     3061 accccaattt ccgataaagt ctttgtgcat cttgattact catacgcact tctaatgaca
     3121 tcgtctcaca gcgattcatt atagcaaagt tctcgatttc gtcaataagc agcgaaccga
     3181 ttcgtttacg ctgatatgcc ggtcgaacgg caatattcgt gatatggcaa tctaaaccga
     3241 acgctcttga accgataaaa ccaatcattc ttccattatc aaagatacct agatatagat
     3301 gaggaagcgg tgacttcaat tctgatataa aggcactttt tgtccatggc aattcgccat
     3361 tatagacttc tcgttcgaca tccagcagct ctttgatatc ttctgtagtc aattctctta
     3421 gtacgtaacg acgatcgatt tgttcgactg ttttttctgt ataaatcatt ttatcttgaa
     3481 aaaagcttct ggcatacgct ctaaattttt tcaacataac tttcgtctcc aggtgtgtgc
     3541 gtctccaacc atttttcttc tgcttctact cgttttaaat agcgaggaag aaaagcttgt
     3601 ttctctgcaa ctggttcaac taaagcacct aattgagcca cagttacacc acttggaata
     3661 tcccatgctg gaactgtatt aatcatcgca ctaggcaata tttcctttat ttcagagaag
     3721 aatttgtgac aatcactccc gacgaaataa atagcttcat tcagcttttg tagttgtgtg
     3781 aacaaatcag aagcagaaat atgtacatct tcaagtacag tttctaatct attttctttc
     3841 cagcggtatg ccccagcgta tacatttttt cttcgagcat caaacatggg cacaataata
     3901 ttatccacac cgatgcaatt agccgcgatg gttttcagac tagatactcc tactagctct
     3961 ttatctaatg tataagcaat cgtttttgct gtcgttacac ctatacgaag ccctgtataa
     4021 gaaccgggac catcagaaac cgcgattcgg tcaatgtctt ttggacttat ttgaagatct
     4081 gcaaacaatt ggtcgattgc aggcatcagt gttacactgt ggttgcgttt gatatttgtt
     4141 tgtatttgtc caagaatttg ttcgtcttct accacaccga tcgccaaagt ttggtttgca
     4201 gtatctattc ctaatgtaat catatatttg atcctttctg ctatgacatt ctcgccttcc
     4261 attgtagcat acttttttat tctgaggatg aaaataccaa aggcaaaact agtacaataa
     4321 tacatgatta tctgctggcg ggcaaatttt gccccttcct gaaacacaaa aaagctgcgg
     4381 tatcactcca ttttcccaca gcttttccat cttttatttc gttgattctc gaataatcaa
     4441 ctctgtttcc acaataatct ttcttgaaac cggtgctttg gaattactta attgaagaat
     4501 cgtctccact gctagctctc ccatccattc ggtataaact tttacagtag aaagcgaggg
     4561 cgttacatat ttcgctacac tgacatcatt gaagcccatc acagaaatat cttctggtac
     4621 cactaaacct ttttcttgaa tcgctttcag tgcgccaatc gctaatgcat cattagctgc
     4681 aaaaagcgca tcaggaacat gcggattcgt ttttaaatac tgcttcactg cttgatatcc
     4741 tgactcgaca gaaaaatctg cttcgataat tggaccttcg tcgtagcctt tttctgccaa
     4801 aacttgttga aatgccataa ttctgggatc ttttagccgc tgatggtttt tcttcgtata
     4861 ttctgttcca gcaagcatag cgatatgttg cgctttatgg gagacaaagt gttcaatgac
     4921 tttttgaaca cttgacttaa agtcgacaac tagagagtcg attccttgag aagtgccgtc
     4981 agaatctacc aaaagcaagg cattttggga tcctttcatt tctaataact gagcttcgtc
     5041 gaattttcct agtgcgatcg ttccatctgc cggaagatca ctaatggaat cccacgtttc
     5101 tttcaataaa gtaacgtttg cttcttctgc ctttttttcg attcctaggc gaatagataa
     5161 ataataaaga tcttcaagct cttccgtatc gtcgtaccac tggatcaggc gaagcgtttg
     5221 cttagtattt gctgcatttt tacgatgttt cgtataattt aagcgttctg ctgcttcaaa
     5281 aattttttgc tttgtggcta agccaacgga aagatcagga tcatagttga gaactcttga
     5341 aacagtagca ggagaaactc ctgccaactg cgcaatgtct ttaattgttg ccatttgtct
     5401 ctcctccttt tttacgttta taaaatcgca gtaaatcgtt caaacgctgc ctgtcctttt
     5461 tcatctcgct tgaatactcc agcgtcttcc agtacacgtg caaaaacctg acctacagat
     5521 ttttgtacga catcggtgac cgtatctttc gtcaaatcag gatgttcttt ttttaattgt
     5581 tctgcccacg gacgatgata ttcagccatc tggttttctt ctcctaataa gaaatgctct
     5641 acttctacta gttctggttt caaacgaggt ggtaaaacag caagccccat tacttcgatc
     5701 aacccaatgt tctccttttt gatatgttgg acatctttat gtggatgaaa aatcccatca
     5761 ggatgttctt ctgaaacatt gttgtctcga aggacaaggt ccaactcaaa gtaattgtcc
     5821 cttcttcgtg caattggcgt aatcgtatgg tgtggtgtac catctgctga aaaagctctg
     5881 atagatactg tttcatccga atatgctttc catttttcca atactgagtt agctgcttca
     5941 ataagcaagt ttttctctgt tgattgcaaa cggatcacag acatcggcca tttcacgact
     6001 cctgcgatga cttgcggata atcttctagt ttgataagtt tttctatcgg tgcttttgcc
     6061 ataggaaatt catgacgtcc tgcctgataa tgatcatgag aaagaatcga tcctccaaca
     6121 attggtaagt cagcattcga accaacaaag taatgaggca agacttcagt aatccgaagc
     6181 agtctagcaa atgtttcctt tgtgattttc atcgggcggt gttgttctga taaaatgatc
     6241 gaatgttcat tgtaataagc ataaggggaa tattggaatc cccagctttc accgtctaga
     6301 ttcattcgaa taatccggtg attggtgcgg gctggatagt tcaagcgacc tctatatcct
     6361 tcgttttcca tgcaaagcat gcaggtagga tagtctactt ttaccgcctc gcgttctgct
     6421 gcgatttgtt ttggatcttt ttctggctta gataaattga ttgtgatttc cagttcacca
     6481 tactccgact ctgccggaaa tttgatgttt tttgcgatgg ctcttgtttt gatgtagtca
     6541 tttttcttac ttaaattaaa gaaatactca gttgcttcct ctgggctttt cgaatagtgc
     6601 tgtgcaaaga atgcattgac gactgaaggt ggcggtgtga gaaaatccat taattgcgct
     6661 tcaaaaatct cttgttctgc tggcaattct gaaagaacac cattttctct tgccttagcg
     6721 acaagctgat ccaataagtc tacagattct tccttaactg gtcttacttc aatgctttct
     6781 agagcatctt ctcctaccat ccctaaaaga cgattctgta aatataagcg gtccatttcc
     6841 atccatccgc cagcttcgat cgcaagtgtg gcaaaatcac aaatcgtttg actgattgac
     6901 atatctcatt cccctattct tcataaccat ttggatggct gacatgccaa tcccatgctg
     6961 ttttgataat cgctttgata tctgtgtatt caggtttcca gcccagaatc tctctcgctt
     7021 tttcacttga agcgactaat cggctaggat ctccagctct tcttggtgct atttctgctg
     7081 gaatttcttt tcctgtcact tctctagctg cttctagcat ttccttgact gagtatcctt
     7141 tgctgctgcc taaattgaag aagttgcttt catttccttc tttcaagtat tccaaagcta
     7201 aaatatgtgc agcaatcaaa tcttctactt gaacataatc tctgatacat gttccatctg
     7261 gtgtatcata atcgtctcca taaacagcca aggcttttct ttgaccaaga gctacttgta
     7321 aaatgattgg gacaagatgc gtttctggtg tatgatcttc tccgatagat gcatctgctt
     7381 ttgctccggc tacattgaag tagcgcaaag cgacatagcg catgccatat gcttggtcac
     7441 accacttcat cattttttcc atcatcaatt tactttcacc atacggattt ttaggattag
     7501 ctggtgtatc ttctgtgatt ggagactctt tcggctctcc ataagttgct gcagtagaag
     7561 agaatacgat tttattcacg ttgaactcgt gcatgacttc aagtaagatt tgcataccgt
     7621 atacgttgtt gttgaaatac atcaatggtt tttcgacaga ttctcctact aaagagcttg
     7681 cagcaaaatg gattactcct tcaatcggtt ccttttcaaa tacggaacgt agaaattctt
     7741 tatcccggat gtctccttca taaaaatgag catctggatg tactgcttgt ttgtgacctg
     7801 tgagcaaatt atctaccaca gctacttgat accccttttg aactagctgg tctacagcgt
     7861 gcgaaccgat ataaccggct ccgcctagta ctaaaattgt cataaaaaac actcctttga
     7921 ttagtaaaat tgtg
//
LOCUS       DAZKLM010000110         7734 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3764
            SAMEA115706913-rid22582563.denovo.115, whole genome shotgun
            sequence.
ACCESSION   DAZKLM010000110 DAZKLM010000000
VERSION     DAZKLM010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706913
            Sequence Read Archive: ERR13196647
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7734)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7734)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287823.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287823.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,004
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,849
            CDSs (with protein)               :: 2,849
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 41 of 96
            Pseudo Genes (incomplete)         :: 52 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 15 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7734
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706913-rid22582563.denovo.115"
                     /isolate="VRE3764"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            99..2426
                     /locus_tag="ACG842_002432"
     CDS             99..2426
                     /locus_tag="ACG842_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBG domain-containing protein"
                     /protein_id="HFQ0248933.1"
                     /translation="MKMIKKTQNKIQRICQKHFVSRLKAVSKRKYLHPIMISIILIFT
                     FLGSLSSIYVNVYTETSNFANQTTEETNSPPYSEIQSIESTNQSSNNETTSTTSSTQL
                     IDPTEKEITAAKAAGEKAYSETGRSQTFSTVAATANFNGHTIPDGYTVIYAKDGVIVA
                     VNGSMSADKTALQGVTKAIAFIDNRPDIHNLFIGSTVNGTTITFPKSIYMGVATAGNT
                     IDSVTPNADKSYSFTPSTWGGLPAGGGMSTFSIGIQLANDIPFTGYSIKNGQIVDGKG
                     NVSNFPTYQAATVQVNANRTGTFSYTVNGGAAKIGTYGTTLSGIHVGDKVVITPTAVT
                     GYSFAQTNGSFTALESNVDTVTYTGKQTPITIKYQDYFGQMIAPSKIMNLTYGSAAQD
                     LTTNTPTINGYTFTAVSAIETKNQSAMSVLASLDTNGNVIVKDANGKQISEVILYYKT
                     NATVSINANGSKYYDGLSVVPIVKYAFNDKTESTSLLNNDLSIDQITWNPTDFKAMNE
                     KGVEVTAPTEIGTYTSWQLTKSGLAKLAARTNYLFTIVQTSDVYTIKQISGAVELSGS
                     KTYDGKAGVPSIHVKLAEGVTSSLTPVQVALSSMDYTADAQSAKNMVNAGSYTIKLTN
                     SGIDKVKAANPKLSFTDIANTSGTYTIYKANAVITVDDAAFNYDAQSHSISVGNLHVT
                     GVVLGESLDYTLTVNSRTDIGSQIVTISLGEGSVNNNYTIIATDTAKLTINPTLLLPS
                     TGGIGLLPFVFLGLIFIFSGFFYFIHRKKKAGEQR"
     gene            2423..3337
                     /locus_tag="ACG842_002433"
                     /pseudo
     CDS             2423..3337
                     /locus_tag="ACG842_002433"
                     /inference="COORDINATES: protein motif:HMM:NF027869.4"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pilin N-terminal domain-containing protein"
     gene            3410..3925
                     /locus_tag="ACG842_002434"
     CDS             3410..3925
                     /locus_tag="ACG842_002434"
                     /inference="COORDINATES: protein motif:HMM:NF037639.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prealbumin-like fold domain-containing protein"
                     /protein_id="HFQ0248934.1"
                     /translation="MDRQTKQKLADAQFLVKKNDQYLAQENGINHWVAKDDSRATIFT
                     SSKDGTFSVHGLSFGSYELVEIKPPKGYILSQSTILFEVEKGSYDPLHPIPLEIINEP
                     KANPTNNGKTPVQMQTNGPSNEKNGGSFPKTSEEMLGGFSILGLLLVLSTGTAWFYKK
                     QQKGNNRREIK"
     gene            3922..4368
                     /locus_tag="ACG842_002435"
     CDS             3922..4368
                     /locus_tag="ACG842_002435"
                     /inference="COORDINATES: protein motif:HMM:NF027869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilin N-terminal domain-containing protein"
                     /protein_id="HFQ0248935.1"
                     /translation="MKNIKTKIWTFLGTAIMLLPFVLGLGTAEVSAAVSPTPENVTVN
                     LHKLKFTSAPENQINNGTELTFPNSEPLNGVEFNVYDITATYYPSKDTAVPADATPFA
                     SVTTSGEGLANLTLPGKSDGKDAVYVFVETPKPGVETSPNIVLSLP"
     gene            4518..5333
                     /locus_tag="ACG842_002436"
     CDS             4518..5333
                     /locus_tag="ACG842_002436"
                     /inference="COORDINATES: protein motif:HMM:NF033902.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HFQ0248936.1"
                     /translation="MTGFDENTGVALYDPAESALTFTTDTEGSISVNGLIDGNYTLVE
                     TKAPDGYQLTNPQKETTFIVKNGKLQQTTGIIHGADEANLGNPNGVVNRENTTTDNTI
                     TVENVQNYGDFHFIKQDVNTQEKLADAEFSILRAKDPTVKLMKKIANLASGEYPYAWS
                     DEVTGSDWEEVKLISDSEGKFSIEGLKQGAYCLQETKAPEGYALPKNEAAYTEFTVDD
                     DPTTSDETTIDNQPKGILPSTGGMGIIVFILVGTALVGGAVIYFKKRHQETEA"
     gene            5539..6711
                     /locus_tag="ACG842_002437"
     CDS             5539..6711
                     /locus_tag="ACG842_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFQ0248937.1"
                     /translation="MNKSRIRILDIFMAIILVVGIGIFSYPFVEDSLNDFLAQQMIIH
                     YQKQASNKNSEEIKKQQEKMTKKNQQLAEQNVSPGIGSFNQAVDAKALKDLPSNAFFM
                     EHMLGVIEIPKINVSLPIFDQTTEIFLQKGTSLLEESSYPTGGKNTHAVLSGHRGLPE
                     AKLFTDLPKLKKGDQFFIQINGKTLAYQVEKIQVVLPDEVDSLGIQKGRDLVTLLTCT
                     PYMVNTHRLLVTGHRIPYQAKEAKKAIQGIDQWKKWKFFIWFIGILLGSIGLVWLLIA
                     YLDSLAIAKRNYPLSFYVKNTNGRPIEGMVFSVKTLNGKHYITREKVPFVKASDEYGL
                     VRFSDLKGRNYRLQHEELLLKIHVKHKHSKQFSMKLKKGRYKLRKEKEVYYLIEKE"
     gene            complement(6873..7367)
                     /locus_tag="ACG842_002438"
     CDS             complement(6873..7367)
                     /locus_tag="ACG842_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0248938.1"
                     /translation="MTEAESALSQAEANPTKENYATASAAIQALPENKQELTSRLAAI
                     DSTIKAKEAEEERKKQEEIARQQAEQAQQEAIAQQQAEQARQEAIAQQQAEQARQQSA
                     AEQASQQAQAAAPEQNNEQTVYVTPTGSKYHTHKCGNGTYTPATLSEAQARGLTPCSK
                     CYGN"
     gene            complement(7364..7558)
                     /locus_tag="ACG842_002439"
     CDS             complement(7364..7558)
                     /locus_tag="ACG842_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0248939.1"
                     /translation="MVAGSLLSGCGATTSTTQSTSSDQASSSSTTISESTADSSKEQE
                     QIEKKKEEKQKAEKAALEKK"
BASE COUNT         2612 a         1455 c         1534 g         2133 t
ORIGIN      
        1 aaaaagcccc aaactttcta tacatcaata aaataataaa aattaaattt agaataacgt
       61 gtaaaggatt atttgatgat tttttattga gaggagggat gaaaatgatt aagaaaactc
      121 aaaataaaat ccaacgtata tgtcaaaaac attttgtttc tagactcaaa gctgtatcta
      181 aaagaaaata cttacatccc attatgattt cgatcatctt gatttttact ttcttaggga
      241 gcttatcaag tatttatgta aacgtttata cagaaacgtc aaattttgct aaccaaacga
      301 cagaggagac aaatagtcca ccctattctg aaatacagtc catagaaagc accaatcagt
      361 ctagcaacaa cgagacaacc tcaaccacat cctcgactca actgattgat ccgacagaaa
      421 aagaaatcac agcagcaaaa gcagcagggg agaaagctta ttcagaaaca ggtagaagcc
      481 agacatttag tactgttgcg gccactgcga atttcaacgg acataccata ccagatggat
      541 atacagtgat atatgccaaa gatggagtca ttgttgcggt taacggatcg atgagtgcgg
      601 ataaaactgc gttacaaggc gtaaccaaag caatcgcttt tattgataac cgtccagata
      661 tccataatct gtttattggc tccacagtaa atggtactac tatcacattc ccaaaaagta
      721 tctatatggg ggtggctacc gctggaaata cgatcgattc agttacaccc aatgcggata
      781 aaagttattc ttttacaccc tcaacatggg gagggcttcc tgccggagga ggtatgagta
      841 ctttttcaat cggtatacag ttagcaaatg atattccatt cacggggtac tcgatcaaaa
      901 atgggcagat cgttgatggt aagggaaatg tttcaaattt tccaacctat caagcagcaa
      961 cggttcaggt caatgcgaat cggacaggaa ctttttctta tacagtcaat ggtggagcag
     1021 caaaaatcgg aacgtacggg actacattat ctggaatcca tgtgggtgac aaagtggtta
     1081 tcactccaac agcagtaacg ggctattctt ttgcacaaac aaacggttct ttcacagccc
     1141 tagaaagtaa cgtcgatacg gtgacctata caggcaaaca aacaccaatc actattaagt
     1201 atcaagacta ttttggacag atgattgctc cttctaaaat aatgaatctt acttacggaa
     1261 gtgctgcaca agacctgacg actaatacgc ctactatcaa cggatatacc tttacagctg
     1321 tttcagcgat agaaacaaag aaccaatcag caatgagtgt tttagctagt ttagatacaa
     1381 atggaaatgt aatcgtaaag gacgcaaatg gaaaacagat ttctgaagtc atcctatatt
     1441 ataagacaaa tgcaacagta tcaatcaatg caaatggcag taagtactat gatggtcttt
     1501 ctgttgtacc gatcgttaaa tatgccttta atgataaaac agaatcaaca tcccttttaa
     1561 acaatgatct cagtattgat caaatcacat ggaatccaac tgatttcaaa gcgatgaatg
     1621 aaaagggagt tgaagtaact gcgcctactg aaattggaac ctatacgtct tggcaattga
     1681 caaaaagcgg actagctaaa ctagctgcca gaacaaacta tttatttact atcgtgcaga
     1741 catctgatgt ctatacgatt aagcaaatca gcggtgcggt agagctcagt ggcagtaaga
     1801 catatgatgg aaaagcaggt gtcccgtcaa tccatgtcaa gctagcagag ggtgtcacta
     1861 gttcgcttac tccagtacaa gtggcactta gcagtatgga ttacacagca gatgcacaat
     1921 cagcaaaaaa tatggttaat gctggcagct atacaattaa actaacgaat agcggaatag
     1981 acaaagtcaa agcagcaaat cccaagttgt catttactga tatagccaac acatcaggaa
     2041 cttataccat ctataaagct aacgcagtga ttactgtcga tgacgctgcc tttaattatg
     2101 atgcccaaag tcactcgatt tcggttggaa atctccacgt taccggtgtt gtcttaggcg
     2161 aatcattgga ttatacccta acagttaaca gccggacaga tattggatca caaatagtaa
     2221 cgattagttt aggcgaagga agtgtaaaca ataattatac gattatcgca acagataccg
     2281 ctaagttgac gatcaatccg actctcttgc ttccttcgac aggtgggata ggtctactgc
     2341 cctttgtctt tttaggactg atctttattt tttctggctt cttttatttt atccatcgta
     2401 agaaaaaagc aggtgaacag cgatgaaaaa aacaaaaaat gtacgtgtgg atatatttgc
     2461 tgttctcttt tttatcctac tggtcttttt tataggttct ccgactgtgt cagctagaga
     2521 atcacctatt acatttactt tgcataatcg aatgttcgaa agcgataaga tgccaagtat
     2581 cagtcaaaac gacggaataa atatcccgga taactttcaa ggagtaaaag ggttgaacgg
     2641 cgttactttc gctatatatg atgtctcgga tgaattttac aagctgcggt cagaaggaag
     2701 tagtgtggaa gacgcacaaa gaaaactagc acaaaaaagt gatagtgaaa aaatactggc
     2761 agaaaatgtg acaaaaacag tagacgagga agagggcatt gcttctttta gtgcatcgga
     2821 caaagatgag caaggtcgtg acgctgttta ccgatttacg gaaataaaga cttcagatta
     2881 agtaaaagaa aaatcagcac catttgtcgt tgtgcttcct gtgacagatt catctaatca
     2941 taaattgacg accatccact tgtatccaaa gagcgaacaa aaaatacctg ctgcactcac
     3001 attaacaaaa acagtagaga ataagcagac agattttgca gatggggata aagtccctta
     3061 tctcatcacc gtaacaattc ctgagaacat caacgagata ggaacctata cgatcaaaga
     3121 tacagctgat cctcagttat ggctagaacc agaaacgatc gattttttga ttggtggaga
     3181 caagattcat acatttcaca cacaaaaaat aaaacatggt tttgtcttat cagattctgg
     3241 aaaagatctt agttcgtttg ctggaaaaaa attaacgatc cgttaccaaa tgacattgaa
     3301 agaaaacagt gaaaaaacga catttgataa cgatgtacgt ttgacgactg acaatcaaac
     3361 agtagaaaca catcttgcca tccaaacagg aggcaaacaa tttgtcaaag tggatcggca
     3421 aacgaaacaa aaactagcag atgctcaatt tctagtcaaa aaaaatgacc aatacttagc
     3481 gcaagaaaac gggataaatc attgggtagc caaagatgat tcaagagcaa cgatttttac
     3541 ttccagtaaa gatggaacat tttctgtcca tggattgtct tttggaagtt acgaactagt
     3601 ggagattaaa ccgcctaaag gatatatatt gagtcaatca accattctct ttgaagttga
     3661 aaaaggcagt tatgatccgc ttcatcctat accattggaa atcatcaacg aaccaaaagc
     3721 aaatccgacg aataatggaa aaacgccagt tcagatgcag acaaatggtc catcaaatga
     3781 aaaaaatgga ggaagctttc caaaaacaag cgaagagatg cttggaggtt tctcaatttt
     3841 agggttattg ctagtccttt caacagggac agcttggttt tataaaaaac aacaaaaagg
     3901 aaacaatagg agggaaataa aatgaaaaac atcaaaacaa aaatatggac gtttttagga
     3961 actgctatca tgctgcttcc tttcgttctc ggcctaggaa ctgcagaggt ttctgcagcc
     4021 gtatcaccta caccagaaaa tgttacggtt aatttgcata aattgaaatt cacttcagca
     4081 ccagaaaatc aaataaacaa cggaacggaa ttgacatttc cgaatagcga accgttgaac
     4141 ggcgttgaat tcaacgtata tgacatcact gctacgtact atccaagtaa agacacagca
     4201 gttccagcag acgctacacc cttcgcaagt gtaacgactt ctggtgaagg attagctaac
     4261 ctgactcttc caggtaaatc tgatgggaaa gatgctgtat acgtctttgt agaaacacca
     4321 aagcccggtg tcgaaacgtc gccaaatatc gtgttaagct taccctagac cctgacaaac
     4381 ctttagacac gattcaccta tatgctaaaa atgtcatcaa agaaagcaat atcaaaatcc
     4441 agaaagctgg gaacttttca ggggtagata agttagcagg ggcgcaattc cagatcaaaa
     4501 attcagagaa tcaattcgtg accggttttg atgaaaacac tggtgtcgct ctctatgatc
     4561 ctgctgagtc agcgttgaca ttcacaactg atactgaagg atccatcagt gtcaatggat
     4621 taatagacgg caattatact ttagttgaaa caaaagcacc agatggttat caattaacca
     4681 atccacaaaa agaaactact tttatagtaa aaaacggaaa attgcaacaa acaaccggta
     4741 tcatccatgg cgcagatgaa gccaatcttg ggaatccaaa cggtgtagtc aatcgggaaa
     4801 atacaactac ggataacacg attactgtag aaaatgtcca aaactacggt gatttccact
     4861 tcatcaaaca agatgtgaac acgcaagaaa aattagccga tgcagaattt tctattctaa
     4921 gagcaaaaga tccaactgtt aaattaatga aaaaaatagc aaaccttgcc tcaggagaat
     4981 acccatatgc ttggtcagat gaagtcacag gttctgactg ggaagaagtg aaattgattt
     5041 ctgattcaga agggaaattt tcaatcgaag ggctgaaaca aggggcatac tgccttcaag
     5101 aaacaaaagc accagaaggc tacgcattac ctaagaatga agctgcgtac actgaattta
     5161 cagtagatga tgatccaaca acttctgatg aaacaacgat cgataaccaa cctaaaggaa
     5221 tcttgccttc aactggtggt atggggatca tcgtattcat cttggtaggt actgctttag
     5281 ttggaggagc agtgatctat ttcaaaaaac gccatcaaga aacagaagct taaactattg
     5341 gaaacaaaaa tcatacgata aataaacgat agtgattcaa atggggagaa aggttgtgac
     5401 aagtggtcgg tctcttggta ttcgctcaac aaacggcaaa ataggaggaa ctattttgcc
     5461 gtttgcttcg ctaatactca ggtcaaggcg ctgatttcac aacctcttca tatacatatt
     5521 agaaaatagg tggacatgat gaataaaagc agaataagaa tactggacat cttcatggca
     5581 atcattttag tcgttgggat cgggatcttt tcttatcctt ttgtagaaga ttcactgaat
     5641 gattttcttg cccagcaaat gatcatccat tatcaaaagc aagcatcgaa taagaattca
     5701 gaggaaataa aaaaacagca ggaaaaaatg acaaagaaaa accaacagtt ggcagagcaa
     5761 aacgtatcac cagggatagg cagtttcaac caagcagttg atgcaaaagc attaaaagat
     5821 ttacctagta atgccttttt tatggaacat atgcttggag tcatcgaaat cccaaaaatc
     5881 aatgtcagct tacctatatt cgatcagaca acggaaatat ttttgcaaaa aggaacgtca
     5941 ttattggaag aaagttctta tccgactggc gggaaaaaca cccatgccgt tctttctggg
     6001 catcgtggac tcccagaagc aaaattattt acagatttgc ctaagttgaa aaaaggagac
     6061 cagtttttca tccagatcaa tggaaagaca ttggcgtatc aagtagaaaa aatccaagtg
     6121 gtcttacctg atgaagtaga ttctttgggt atccaaaaag gcagagatct cgtgactttg
     6181 cttacctgta ctccttatat ggtcaataca catcgactgc ttgtgacggg gcatcgtatt
     6241 ccttatcaag caaaagaggc aaaaaaagcg atccaaggaa ttgatcaatg gaaaaaatgg
     6301 aaatttttta tttggtttat cggtatacta cttggatcaa tcggcttagt ttggctactc
     6361 atcgcttatt tagattcttt ggccatcgct aagcgaaact atcctttatc tttttatgta
     6421 aagaacacaa acggacgacc aatcgaagga atggtctttt ctgtaaaaac gctcaacggc
     6481 aaacactata ttactagaga aaaagttcca tttgtgaaag cttctgatga atacggacta
     6541 gtgagatttt ctgatttaaa aggcaggaac tatcgcttgc aacatgagga gctcctgctt
     6601 aaaatccatg taaaacataa acattcgaaa cagttttcaa tgaaattgaa aaaaggaaga
     6661 tataaactgc gaaaagaaaa agaggtgtac tatttgattg agaaagaatg aatgaagatg
     6721 tccaaaaaag cagagggaac agactaaatg aaaaaagaga gaagctgacg aaatggacgt
     6781 gtaaactgtt atttttggtt tactatctgg aacgccatct tctatttttt tatgtttcag
     6841 aagaatttta ttattatagt ataacttttg aattagtttc cataacattt ggagcaaggg
     6901 gttaaaccac gagcttgagc ttcgcttaaa gtggcagggg tatacgtacc atttccacat
     6961 ttatgtgtat ggtattttga accggtcggt gtgacataga cagtttgttc attattttgc
     7021 tcaggagctg cggcttgtgc ctgctgagag gcctgttcag ctgctgattg ctgtcgcgcc
     7081 tgttctgctt gttgctgtgc gattgcttct tgcctcgcct gttctgcttg ttgctgtgcg
     7141 attgcttctt gttgcgcttg ttctgcttgt tgtcgtgcaa tttcttcttg ctttttccgt
     7201 tcttcttctg cttcttttgc tttgattgtg gaatcaattg cagcaagacg gcttgtcaac
     7261 tcttgtttgt tttcaggtag cgcttggata gctgctgaag cagttgcata gttttctttt
     7321 gtaggatttg cttcagcttg tgacaaagca gattccgctt ctgtcacttt ttttctagtg
     7381 cagctttttc cgctttttgt ttctcttctt ttttcttttc aatttgttct tgttctttcg
     7441 atgaatctgc cgtactttcg gagatcgtgg tactgctgct ggaagcctga tctgaacttg
     7501 tgctttgagt agtagacgta gtagcaccac acccactcaa caaacttcct gcgaccaaaa
     7561 acaatgcaac taattttttc gctaaatttc ctccttaagt aaaaaacaac cgttttcaat
     7621 aaatgtcctt ttcaattgtt tacttacttg ttataataat tgtgttatga atagtgattg
     7681 aggaaactta atctaatccc gtgttgatac acgggatttt ttgctatagt tctg
//
LOCUS       DAZKLM010000111         7599 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3764
            SAMEA115706913-rid22582563.denovo.116, whole genome shotgun
            sequence.
ACCESSION   DAZKLM010000111 DAZKLM010000000
VERSION     DAZKLM010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706913
            Sequence Read Archive: ERR13196647
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7599)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7599)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287823.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287823.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,004
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,849
            CDSs (with protein)               :: 2,849
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 41 of 96
            Pseudo Genes (incomplete)         :: 52 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 15 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7599
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706913-rid22582563.denovo.116"
                     /isolate="VRE3764"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(136..426)
                     /locus_tag="ACG842_002440"
     CDS             complement(136..426)
                     /locus_tag="ACG842_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0248940.1"
                     /translation="MGEKKIKGAGILLVMSKALFLLPCTFMFIVLCYFKRRKKWCWID
                     VLLFLPFFPLIYFLEEIWDDMACDSAFLFSHSLGLMISLTIFAIVLFFLNKK"
     gene            complement(544..804)
                     /locus_tag="ACG842_002441"
     CDS             complement(544..804)
                     /locus_tag="ACG842_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0248941.1"
                     /translation="MGVDHLIVLSKLLFWIPFIIMTSAFFYLFKRPNKYLLLSACPMI
                     YFIHKIRGFYYYDSGISLFCYEIGLLASAIFFIFVLEYFRKK"
     gene            complement(941..1354)
                     /locus_tag="ACG842_002442"
     CDS             complement(941..1354)
                     /locus_tag="ACG842_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HFQ0248942.1"
                     /translation="MANVPSIRDMFPDFNDVFSPAFNDFLGVSSYPKVDLVENEKEYK
                     LTADMPGCDKEDTTVEYSDNTLTISANHESHTEDKEDGNYVRKERHSVSYKRSFYLPN
                     VDEEKITGTFKNGVLKLVLPKTAYQPKETKKIELN"
     gene            complement(1810..2295)
                     /locus_tag="ACG842_002443"
     CDS             complement(1810..2295)
                     /locus_tag="ACG842_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0248943.1"
                     /translation="MHKGIYAGLLGTVILFSACGYKTKNSVKESSAAISTTEVGSTKS
                     TSTTSITNTSTGNVDSQHSQSSIQSFSISSTSEMQESTVPSLPLENSDKALLFLIAHR
                     SELQSDDIVISFYQKIDRDYLFTASSKQIRSQGGSGSVGFYRVSPEGSITMTDANGTP
                     F"
     gene            complement(2434..2649)
                     /locus_tag="ACG842_002444"
     CDS             complement(2434..2649)
                     /locus_tag="ACG842_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF378 domain-containing protein"
                     /protein_id="HFQ0248944.1"
                     /translation="MKTLDAIALTLLIVGGLNWLLVGLFELDLVAMIAGGSTTIFAKI
                     IYIVVGICAIYCLKFFPMITRKVDERY"
     gene            2873..3673
                     /locus_tag="ACG842_002445"
     CDS             2873..3673
                     /locus_tag="ACG842_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFQ0248945.1"
                     /translation="MSDWLDIAGKVVIVTGGSSGIGRSIVENLLKQNAQVANFDVTEC
                     RIQHENLLSLKVDVSSKTDIEEGIYKVMKHFKTIDGLVNNAGINIPSLLIDRNHPKSK
                     YELSEQVFDKMIAVNQKSVYLMSQAVGRILVQKGSGVIVNLSSESGLEGSEGQSCYAA
                     TKAAMNSFTRSWAKELGKRNVRVVGVAPGILEETGLRTQEYEEALSYTRGISVEQLRN
                     GYSRTSTIPLGRSGKLQEVADLVCYYLSERSSYITGVTTNISGGKTRG"
     gene            3692..5560
                     /locus_tag="ACG842_002446"
     CDS             3692..5560
                     /locus_tag="ACG842_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HFQ0248946.1"
                     /translation="MTLVNRWYQLLKLLVTHKKVSIQEVQKEIKTSNQTIKKDIGELN
                     EEIAGIAKIIQKNKHFELEIYDFDFFDEIMQGKLKTASDFNSASKRIAYLLNRLIETE
                     DFLRMDDLSEEVGVSRGTVVKDLKTLKEMINDFDVKITGTPNKGLRIIGDELELRLLL
                     FYFVSPYFSETKTEQFLEEKQAIKHFQKKTNASKQEITLLTKVIGISLNRISSNYLLK
                     KPINRYSGCFEYIQEFNELINQLEEIYELTLSQYEKDFISFPLNIINTSITMIRKANS
                     DLRLYFDPMMMRIRRLDLIDLDESFLYQEMKQHLSLLINRVIFRYRLTDLFYGEIEKK
                     YPFSYQIASECIQALEAILQHKISKVEISYLTLYLELAFRYQKEHFSSKKVAIVHTTG
                     KGTALMIQRQIKRILGRDIQITHVSEEQYEKVDLNKYFAVFTTIPLKNVNEETPVIHL
                     TNLFDDSWLRAQWQKAKKKRLAGFHSTVFFYQIWKEKKTYQEYLNKLVAPLIRGYYVD
                     EEFEKRILKREYQQSTFVNKEFAFPHTTNQLSQHIVLSVGLITDQGLAETGGNGVIFL
                     FAIPEEMTAEVENELFELYDILFELIKMGIDEFIELAPTQESFLKLIKSKGERLHE"
     gene            5553..6044
                     /locus_tag="ACG842_002447"
     CDS             5553..6044
                     /locus_tag="ACG842_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GutM"
                     /protein_id="HFQ0248947.1"
                     /translation="MNDMYLFIVFAASAYVLQAVISWKQLKHFNQTYSELRRKGKVAI
                     GKRPGKIRSGTIVMLAIDDHDQVLEVRKMQGVTSLAKFHSLPVYAGEDIHYIDRYHPS
                     VRAEDRLTQAALENAREVYLRIEAGNEKSEQPSLPFIAMLNNFRIWKSTIKEKRSDKR
                     WIS"
     gene            6032..6586
                     /locus_tag="ACG842_002448"
     CDS             6032..6586
                     /locus_tag="ACG842_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HFQ0248948.1"
                     /translation="MDIITKFAEGFMRLFQTGAETFISWMTGIVPVVLMLMVAMNTLI
                     AFLGEDRVTKVAKLSAKNPLTRYLVLPFLSAFMLGNPMSFTMARFLPEYYKPSYYAAQ
                     AQFCHTSNGVFPHINPGELFVWMGIAQGIETLGLNSMELAIRYLLVGLLMNFIGGWIT
                     DFTTGFVCRQQGIVLSKKVELSVD"
     gene            6604..7599
                     /locus_tag="ACG842_002449"
     CDS             6604..7599
                     /locus_tag="ACG842_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HFQ0248949.1"
                     /translation="MYGDIKIVRGNGGYGGPLIIRPTKDKHKFIYVTGGGERPMIVDK
                     IEELTGMESINGFKTSIPDSEIALAIVDCGGTLRCGIYPKKGIPTINIVSTGKSGPLA
                     QYITEDIYVSAVGLEQIQPLMETDDNKETVNTASPDKKYKYDTSKKITEQKAEQQNWI
                     AKIGMGAGKVVATFNQSAREAVDTMIRTVIPFMAFVSLLIGVIQGSGIGNVFANIMAP
                     LAGNAVGLTIIGFICSLPFLSPLLGPGAVISQVIGTLIGVEIGKGNIPPQLALPALFA
                     INTQNACDFIPVGLGLEEAKAETVEVGVPSVLYSRFLNGIPRVIIAWVASFGLYS"
BASE COUNT         2708 a         1213 c         1443 g         2235 t
ORIGIN      
        1 tgttactatg aataacataa taagaaagta aagtggattg atttgagatc tttttatact
       61 tcaaaaaaga taacacccaa ctttcaaaaa tatgttttgg aagttgggac aaacgagtta
      121 atacatttta atacattatt ttttattcaa aaaaaatagc acaatggcaa agatcgtaag
      181 agagatcata agccccaatg aatgactaaa aagaaaagca gaatcacatg ccatatcatc
      241 ccatatttct tcaagaaaat aaataagcgg gaaaaaggga aggaatagta gaacatcgat
      301 ccagcaccat ttttttcttc ttttgaaata acataaaaca ataaacatga acgtgcaggg
      361 aagtaaaaac aaagctttac tcataactaa caaaatacca gctcctttta tttttttctc
      421 ccccacattc aaagagtaac aatggtatgt acttaataat agaaaaatgg atcaacttgt
      481 atgaataaca gctgaaaaga tctgcaccaa ataaaaaact tccagccaaa actgactgga
      541 agtttatttt tttctgaaat attctaacac aaatatgaaa aagatggccg aagctaataa
      601 gccgatttcg tagcaaaaca atgagatacc agagtcgtaa taataaaatc ctctaatctt
      661 atggataaaa tagatcatcg gacaagcaga taagagtaag tatttgttag gccttttgaa
      721 taagtaaaag aaagcactgg tcattatgat aaatggtatc caaaacaaaa gtttacttaa
      781 gacaatcaaa tgatcaactc ccatattttt taccaagaga gatcataaca tctttatcgt
      841 aaaaacaaaa tatttttttg ctcattttga aaatttaaac atactcgttc ttatccaaat
      901 atataaaaat cgcccctcat tcatcgagag gcgataagtt ctaatttaac tcaatttttt
      961 ttgtttcttt tggctgataa gctgttttag gtaataccaa tttcagcaca ccgtttttaa
     1021 aggttccagt gattttttct tcatcaacat taggaagata gaatgaacgt ttgtaagaga
     1081 ccgaatgtct ttctttccgt acatagttgc catcttcttt atcttctgta tgggactcat
     1141 gattggcgga gatcgtcaaa gtattatctg aatattcaac tgtcgtgtct tctttgtcac
     1201 agccaggcat atcagcagtt agtttgtatt ctttttcatt ttcgactaaa tctacttttg
     1261 gataacttga aacacctaaa aaatcattga atgctggact aaacacgtca ttgaaatcag
     1321 ggaacatgtc gcgaatgcta ggtacatttg ccatttttga tcatcctttc aataagtatt
     1381 ttttatttat ccctatttac gtaataactc tattttgaaa aaaatgcaag tataatggtt
     1441 agctttgtga atatttcatc taatacgata caaaggctat ctttctaatt tgaaaaaggg
     1501 atcattaaaa taataagaag aaaatatgtc aaaaggtaaa aaagtaaatt cttctatcca
     1561 aatatagaaa aattgaatct agaaaaccgc ccgacaataa atttcattaa ctcagttgac
     1621 cgaaaatgaa gtagatacca aaaatttatc aagagagcaa gtagagaagt gagtatattc
     1681 ataagagaaa tagctgtaac gaattattag gttaatgcaa atgcttattt atactccgta
     1741 taactttctc agaaaaaatg ataccaataa tgacttgcta gtactgtaaa tccgtataat
     1801 atcatctaat caaaatggtg ttccatttgc atctgtcatc gtaatcgatc cttcgggtga
     1861 cactcgataa aaaccaacgc ttccagatcc tccttgggaa cggatttgtt tgctggaagc
     1921 tgtgaacaaa taatctcgat caattttttg ataaaagcta ataacaatat cgtcactttg
     1981 taattcggaa cggtgggcaa taagaaatag aagtgctttg tcagagtttt ctaatggaag
     2041 agaaggtaca gtgctttctt gcatttcaga tgttgaagaa atgctaaacg actgaatact
     2101 tgattggcta tgttgcgaat ctacattacc agtagaagta ttagtaatgg aagtagtaga
     2161 agtactttta gttgaaccaa cctcagttgt tgaaatggct gctgaagatt ctttaacaga
     2221 attctttgtt ttatatccac aggcagaaaa aagtattaca gttcccaaca agcctgcgta
     2281 aatcccttta tgcatgatag ctcctcctaa aatagattat ctttattcag tatatcactt
     2341 aaacaaagaa aagcagatgg tttaaaatga aaaaaccttc ccaataaagg gaaggttcat
     2401 gaagataata acttttttaa taattatagc tttctaatat ctttcatcaa cttttctagt
     2461 aatcattggg aaaaatttca agcagtagat cgcacaaata cctacgacga tataaatgat
     2521 cttcgcaaaa atagttgttg aaccaccagc aatcatagct actaaatcaa gttcgaacaa
     2581 gcctaccaac agccagttca accctcccac aatcaataaa gttaatgcaa tagcatctaa
     2641 agttttcatt agaattctcc tttcttaaat gataaaacaa ttatatatca aaaagagaaa
     2701 cattgcttat ataacgctca ggaattttag caatctatgc aaaaagatga aaattttttt
     2761 tgctcttcca ttagtacaca ttttttagct gaagctgtaa atttcatgtc ttaatttttt
     2821 ttagtgagct tttatactgg atttgtaaga aaaaagaagg agaggatcac atatgtcgga
     2881 ttggttggat attgcaggaa aagtagtaat tgtaactgga ggttcctcag gaattggaag
     2941 aagtatcgtc gaaaatttgt tgaagcagaa tgcacaagta gcgaattttg atgtaactga
     3001 atgccgtata cagcatgaaa acctactatc tttaaaagtc gatgtgtctt ctaaaacgga
     3061 catagaagaa gggatatata aagtaatgaa acatttcaag acgatcgatg gtctagtcaa
     3121 taatgcagga ataaatattc cttctcttct aatagataga aatcatccaa aaagcaagta
     3181 tgaattatca gaacaagttt ttgataagat gatcgctgtc aatcaaaaaa gtgtatattt
     3241 gatgagccaa gcagttggaa gaatcttggt gcaaaaggga tcaggagtga tcgtgaatct
     3301 ttcttctgaa tcaggattgg aaggatcaga aggacagagc tgttatgctg ctacaaaagc
     3361 agcgatgaac agttttactc gttcttgggc aaaagagtta ggaaagagga atgttcgtgt
     3421 ggtaggtgtt gcaccaggga tactggaaga aaccggtttg agaacacaag aatatgaaga
     3481 agctctttct tatacgagag gtataagcgt cgaacagctg agaaacggtt acagtcgaac
     3541 aagtacgatc cctctaggaa ggagtggtaa gctgcaagaa gtggcggatc tagtctgcta
     3601 ttatctttca gaaagatcaa gttatatcac tggagtcacg actaatattt caggaggtaa
     3661 aacaagagga taaaggatag gggggacgca cgtgacgcta gtaaacagat ggtaccaatt
     3721 attgaaatta ctagtcactc acaagaaagt ttcaatccag gaagtgcaaa aagaaatcaa
     3781 aaccagtaat caaaccataa aaaaagatat tggggagttg aatgaagaaa ttgccggtat
     3841 tgcaaaaatc atccaaaaaa acaaacattt tgaacttgaa atttatgatt ttgatttttt
     3901 tgatgagatc atgcaaggaa aattgaaaac agccagtgat ttcaattcgg catccaaaag
     3961 aatcgcttat ttactaaata ggctgatcga aacagaggat ttcttacgta tggatgattt
     4021 gtctgaagaa gtcggtgtca gcagaggaac agtggtcaag gatctaaaga cattaaaaga
     4081 gatgatcaat gattttgatg taaagattac aggcacacca aataaaggac taagaataat
     4141 aggagatgag ctagaattac gactactttt attttatttt gtttctccct acttttcaga
     4201 gacgaaaaca gaacagtttc tagaagaaaa acaagcaatc aaacattttc agaagaagac
     4261 gaatgctagt aaacaagaaa tcaccctttt aacgaaagtt atcggtattt ctttgaatag
     4321 aatctctagt aactatctgt tgaagaagcc catcaatcgt tacagtgggt gttttgaata
     4381 tatccaagaa ttcaatgaac tgatcaatca gttggaagaa atctatgaac taactttgag
     4441 ccaatatgaa aaagatttta tcagttttcc tttgaacatc atcaacacaa gtataacgat
     4501 gataaggaaa gctaactctg atttgcgctt atatttcgac cccatgatga tgcgtattcg
     4561 acgattagat ctcattgatc tggatgagtc cttcttatat caagagatga aacagcacct
     4621 ctctttattg ataaatcggg taatttttcg ttatcgattg actgatttat tttatggtga
     4681 gatagaaaaa aagtatcctt tttcttacca aatcgctagc gaatgcatac aagcattaga
     4741 agctatcttg caacataaga tctctaaagt agagatcagc tacctaacgt tgtatttgga
     4801 gttagcattt cgatatcaaa aagagcattt ttcctctaaa aaagtagcca ttgttcatac
     4861 gacaggaaaa ggaacagcat taatgatcca aagacaaatc aaaaggatat tggggaggga
     4921 tatacaaata acacatgttt cagaagaaca atatgaaaaa gtagatctaa acaaatattt
     4981 cgctgttttt acaacgattc cgttaaaaaa tgtaaatgaa gagacaccgg tgatccattt
     5041 gactaatctt tttgatgact cttggttaag ggcacaatgg caaaaagcaa aaaaaaagcg
     5101 attagccggg tttcattcta ctgttttttt ctatcaaata tggaaagaaa aaaagacgta
     5161 tcaagaatat ttaaacaaat tagtcgctcc tttgatcaga ggctattatg tggatgaaga
     5221 atttgaaaaa aggatcttga aaagagaata tcagcaaagc acatttgtta acaaagaatt
     5281 tgcttttcct cacacaacca accagctatc tcaacacatt gttttatcag tagggctaat
     5341 aactgatcaa ggtttagcag aaacaggagg aaatggagtt atttttttat ttgccatacc
     5401 tgaagaaatg acggctgaag ttgagaatga attatttgag ctatacgata tcctatttga
     5461 actcataaaa atgggaatag atgagtttat tgaattagca ccgacacaag aatcattttt
     5521 aaaactaatc aaaagtaaag gagagagact tcatgaatga tatgtatcta ttcatagttt
     5581 tcgcagcttc agcttatgtg ctgcaagcag ttatcagttg gaagcaacta aagcatttca
     5641 atcaaacata tagcgaactg cgaagaaaag gaaaagtagc gattggtaaa cgacctggga
     5701 aaatccgttc aggcacgatt gttatgctag ctatagacga tcatgatcaa gttttagaag
     5761 tcagaaaaat gcaaggggtt acatctttgg caaaatttca ttcattacct gtctatgcgg
     5821 gtgaagatat ccattacatc gatcgttacc atccctcagt tcgagcggaa gatcgtttga
     5881 cacaagcagc tttagaaaat gccagagaag tatacttacg aatagaggca ggaaatgaaa
     5941 aaagtgaaca accttcattg ccttttattg ctatgctcaa taattttcgg atatggaaaa
     6001 gtacgatcaa agaaaaaagg agtgacaaac gatggatatc ataacaaaat ttgctgaggg
     6061 atttatgagg ctgttccaaa caggtgcaga aacattcata agctggatga caggaatcgt
     6121 accagttgtc ttgatgctga tggttgcaat gaatacgctg attgcttttt taggtgaaga
     6181 ccgagtaacg aaagtagcca aattatcagc gaaaaatccg cttactcgtt accttgtgtt
     6241 accgttctta tctgctttta tgttgggaaa tcctatgtca tttacaatgg ctcgtttcct
     6301 tccagaatac tacaaaccaa gctattatgc tgctcaagct caattttgtc atacgagcaa
     6361 tggtgtattc ccccatatta atcctggaga attgtttgta tggatgggga ttgctcaagg
     6421 gatcgaaacc ttagggctaa attcgatgga gctggctatc cgatacttgc ttgtgggact
     6481 gctgatgaac tttattggtg gttggataac tgattttact actggatttg tatgcagaca
     6541 gcaaggaatc gttcttagca aaaaagtaga gctttcagtt gattagagaa aggagagttt
     6601 taaatgtatg gtgatattaa gatcgtaaga ggaaacggcg ggtatggagg tcctttgatt
     6661 attaggccga caaaagataa acacaagttc atctatgtca caggtggagg tgagaggcca
     6721 atgatcgtag ataaaataga agaactgaca ggtatggagt ctatcaatgg gttcaaaaca
     6781 tcaatacctg atagtgaaat cgcgcttgca atcgttgact gtggagggac tcttagatgt
     6841 ggaatttatc ctaaaaaagg gataccaacc atcaatatcg tttccacagg aaaaagtggc
     6901 ccattagcac aatatatcac tgaagatatt tatgtttcag cagtagggtt agaacaaata
     6961 cagccactaa tggaaactga tgataacaaa gaaacagtaa atactgcatc tccagataaa
     7021 aagtacaaat atgatacttc taaaaagata accgagcaaa aagcagagca gcaaaactgg
     7081 atagcaaaaa ttggaatggg cgcgggaaaa gtagttgcta cattcaatca atcagcaaga
     7141 gaagcagtag atacgatgat ccggacagtg atccctttta tggctttcgt ttcccttttg
     7201 attggtgtga tacaaggttc tgggatcggt aatgtatttg ctaacattat ggcaccttta
     7261 gcaggaaatg cagttggatt aacaatcatt ggctttattt gttcactgcc atttttatct
     7321 cctttgcttg gtccaggggc agttattagc caagtgattg gaacattgat cggtgtagag
     7381 attgggaaag gaaatattcc tccccaatta gcattaccgg cactatttgc tatcaatact
     7441 caaaatgcat gtgattttat tccagtcggt ctaggtttgg aagaagcaaa agctgagaca
     7501 gtagaagtag gtgtaccatc agttctttat tcaagatttc ttaacggcat cccacgagtt
     7561 atcattgctt gggtagcaag ttttggatta tatagctga
//
LOCUS       DAZKLM010000112         7537 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3764
            SAMEA115706913-rid22582563.denovo.117, whole genome shotgun
            sequence.
ACCESSION   DAZKLM010000112 DAZKLM010000000
VERSION     DAZKLM010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706913
            Sequence Read Archive: ERR13196647
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7537)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7537)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287823.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287823.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,004
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,849
            CDSs (with protein)               :: 2,849
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 41 of 96
            Pseudo Genes (incomplete)         :: 52 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 15 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7537
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706913-rid22582563.denovo.117"
                     /isolate="VRE3764"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            107..1248
                     /locus_tag="ACG842_002450"
     CDS             join(107..335,335..1248)
                     /locus_tag="ACG842_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611172.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFQ0248950.1"
                     /translation="MAKAKKKFDEDFKKMILDLNQSGQSVEELAAQYGIATQTIYRWK
                     KLHTKNVATGMTEAEILAMKKEMARMQEENTIPKKGFNHIRSKVKMSDVFEFIQSNVH
                     DHDVKQMCTVLEAPKSSYYDWKKKKPSKRQSENKRLNRLISGIYFENKGIYGAPKIHK
                     ILVGRGETLSLKKVQILMRKLGLRSVTLKKYKPGKQAKVKSEGRKNLLNQDFTTTSIN
                     QKWVTDITYIHTLKDGWTYLSTIQDLHTKKVIGWKFGKQMTKELVIETLDNALLNQTP
                     SENLIIHSDLGSQYTSEAYEAKLNELNIRHSFSRKGCPYDNAGIESFHASLKKEEVYP
                     SKAYENFEAAYLALFQYIEGFYNRKRIHSSINYLTPNQMEELALQA"
     gene            1483..1935
                     /locus_tag="ACG842_002451"
     CDS             1483..1935
                     /locus_tag="ACG842_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000751236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0248951.1"
                     /translation="MKKTILSIFSVFTILIGGYFLLNLDSVNSNAQIKDQRVAEHNNK
                     KMSLTKNIYSMSDEELYKEFGDLSGEQLEESIPGISDSDKIVVLSAGGQLMGDGKSEL
                     VSYKQNHRQFIDVNTDSNAISIKDLKELLNSKRNELKQIKETNSVELE"
     gene            1949..2638
                     /locus_tag="ACG842_002452"
     CDS             1949..2638
                     /locus_tag="ACG842_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000718009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0248952.1"
                     /translation="MKKIFGVSVGLLMMLTFGNAVYAAEKEVISDPEQYASRYFADFE
                     EEDKVLALPEGGFLKGEAKIVSMDNPDIIQGSYNSETDPASVSVTEAKKIVAEKFKND
                     IETTPIMPRGAFPPDVGTSGHLLVLAGGGSYKSQPFSGAGWRFGGMGVKPADGTGNFL
                     RWKSYIDDGRVGNVNQANATKSGSIQGMALYAREGFKYFAPNGPGWTMNIGIYYTYNP
                     VAGTYYVVQNI"
     gene            2763..5114
                     /locus_tag="ACG842_002453"
     CDS             2763..5114
                     /locus_tag="ACG842_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase domain-containing protein"
                     /protein_id="HFQ0248953.1"
                     /translation="MDEMVLSTQKWLNKKYSNVTGFDKVPENGRTGWPTIYGLIEGLQ
                     VELGITNLVANFGPTTEKMYDNQVTPKWGKNLPKNIVFLIQGAFWCKGINPGGFDGVY
                     TPYLDTAVKELQTDAGFNGSAVTGVLDAKWAKALFDMSAFVLVPGGDSKIRKMQQWLN
                     VNYLEYTGIMPCDGIYQRNSNQALIFALQAELGYSPSEATGSYGNGTTSKTYPVSEGN
                     SGNYVRIIQYGLYVNGYFEDGTFDGIFTKYMGLEVLAFRKFMVLPEYTEIADVVVIKG
                     LLSSAGDIRRSADGADTSTQLTRSQIQTLVDNDIKIVGRYLTGTVGIGKDERNKYLTT
                     EELNNIFSKNLSVFPIYQDGGAALAYFTYDRGLSDGKKAIDAAKNLNIPLTTVIYFAV
                     DLDMLGEEILAYAGEYFKGVSAVMSYSGYQTGVYGTRNVCSQIINNGYASFAFVSDMS
                     TGYSGNLGFPMPRQWSFDQFVEFTIGSGNGAVGIDLIATSGRDSGFNELSNDTNNDNY
                     IAKYNQKVINQMVRAYQYLSQAGLDNPWNPHLTFYRYVNYSGLSWDIISSPVTEHDRK
                     IYDEYRNKLTNSEGLYNYFIDPNTGSIIGLPHLIVTLQSQMFLADTINDSVSDFAGWL
                     GDLMTCWGEVKKLGIQMEQGVFALVGTAAGGTFSLEDLYQDVDAVNVYTLAKRNSDST
                     ALSALKTYYCDKGAIDRFNQFVKNRFGSSDKIASVTNSLLENDETVDILGVGIISFFI
                     DKIVNNSLGTDFTYLDFIGDEGKVANGFSEKIIQFQENRSKLN"
     gene            5194..5589
                     /locus_tag="ACG842_002454"
     CDS             5194..5589
                     /locus_tag="ACG842_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0248954.1"
                     /translation="MMIMEEIEWNNLTQNNKNNSGCGIIFMFVIVLGIVVLLITVKIV
                     DYFANTPEEQIQRKLPEFERVPPYDPRDNSFQVKKLKESPNEMWFEVTVESSDFKEKK
                     KEYNYIVKAKVSKRFIFNPKLEIYSVVIE"
     gene            5914..6531
                     /locus_tag="ACG842_002455"
     CDS             5914..6531
                     /locus_tag="ACG842_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFQ0248955.1"
                     /translation="MRKFFYIRVSSYDQSIERQLISAKELGIPEEYIFVEKASGKDFK
                     RPEYQLMKRMFRKGDLLYLQSLDRLGRNKQMILEEWRELTKEKQIDISVLDMPLLDTA
                     SYKQLDGLETLISDLILQLLSYMAEDERKRINSRQREGVEAAKQKGVKFGRKKIALPP
                     EFPAIYSDWKAGKITAVYAMDQLGMKRNTFYRRVKEYEHTLHHFS"
     gene            6572..6871
                     /locus_tag="ACG842_002456"
     CDS             6572..6871
                     /locus_tag="ACG842_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000366408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BRCT domain-containing protein"
                     /protein_id="HFQ0248956.1"
                     /translation="MDLTNQRVVFTGTLQQMTRTQAIGLVHSLNGHCQSSVTSKTNFL
                     VCGQYSKSLFDDSLYTKKEQTARDLIALGHEITILSEVEFYALISQQLKEWQRYL"
     gene            6905..7072
                     /locus_tag="ACG842_002457"
     CDS             6905..7072
                     /locus_tag="ACG842_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000824191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFQ0248957.1"
                     /translation="MKRKSYDRWGIEITSFNRNIAFHVECDCGELAKSVYQKNYFYCP
                     KCHRRYGVHGT"
     gene            complement(7117..>7537)
                     /locus_tag="ACG842_002458"
     CDS             complement(7117..>7537)
                     /locus_tag="ACG842_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFQ0248958.1"
                     /translation="SLDRLGRDYEDIKNTVAFMKQKKVALKILDAQFLDFNTGNELLD
                     TAMFDMFLSLLSYIAQNEREKIRERQRQGVLLAQKAGRYKGRPIEYSINSTDPQKRLV
                     FKTVVDMLKQGIPVAEIAKENGISRPTIYKIKKANDL"
BASE COUNT         2594 a         1138 c         1502 g         2303 t
ORIGIN      
        1 attcctcctg ctatcttttt ctattattct atcttatttg atagtagatt taaaactttg
       61 caacagaacc gtagaatcta actactaaga aaaggacgtg tccacgatgg caaaagccaa
      121 gaaaaaattt gatgaagact ttaagaagat gatcttagat ttaaaccagt ctggccaatc
      181 ggtcgaagaa ctggcagcgc aatatggtat cgctacacaa acgatttatc gttggaagaa
      241 gcttcataca aagaatgtag caaccggcat gactgaggca gaaatcctag cgatgaaaaa
      301 ggaaatggcc cgcatgcagg aggaaaacac tatcctaaaa aaggctttaa ccatattcgc
      361 tcaaaagtaa aaatgagtga tgtcttcgaa tttattcaat caaatgttca tgatcatgac
      421 gtaaaacaaa tgtgtaccgt tttggaagca ccgaaatcca gctactatga ctggaagaaa
      481 aagaaacctt ccaagcgcca atcagaaaac aaacgcttaa accgcttgat ttcaggaatc
      541 tactttgaaa acaagggtat ctatggtgcg cctaaaattc ataagattct tgttggccgt
      601 ggcgagacct tgtccttaaa gaaagtacaa atactgatga gaaaattagg cttgcgttca
      661 gtcaccctca agaagtacaa gcctggcaaa caagcgaaag ttaaaagtga aggacgtaaa
      721 aacctgttaa accaagattt taccactacg tcgatcaatc aaaaatgggt gacagatatt
      781 acctatattc ataccctaaa agatggctgg acctacctct ccactattca agacctccac
      841 acaaagaaag tcatcggttg gaagtttggc aagcagatga cgaaagaatt ggttatcgaa
      901 acccttgaca atgcgctctt aaaccaaaca ccctccgaga acttgatcat ccactcagat
      961 ttgggttctc agtacactag cgaagcctat gaggcaaagc tgaatgaatt gaacattcgg
     1021 cactccttca gtcgtaaggg ctgtccctac gataatgccg gtatcgaatc gtttcacgcc
     1081 tcactcaaaa aagaagaagt ctatccatca aaagcgtatg aaaactttga agctgcttat
     1141 ttagcgttat ttcaatacat tgaaggattt tacaatcgca agcgaatcca tagtagtatc
     1201 aactatttaa caccaaatca gatggaggag ttggcattgc aagcttagtt cctagctacc
     1261 acttacattt ttttggcgag atttccacag ggtttcaact tcatcgcttc gatcagcggt
     1321 caaacggttt tggtttgacc gctggtcgaa acgatgagat actcacccgc tggaatctcg
     1381 aaaaaattct cacaaatcgt gtccgaggta ttgactcaaa tccaccaatt atcttatcgt
     1441 agatatatat tataaattta ataagaaagc gaggtgagaa tcgtgaaaaa aacgatacta
     1501 agtatttttt cagtatttac aatattaatt gggggctatt ttcttttgaa tttagattct
     1561 gttaattcaa acgctcaaat aaaagatcaa agagtagctg aacataataa taaaaaaatg
     1621 agtttgacca aaaatatcta ttctatgtca gatgaagaat tgtataaaga atttggtgat
     1681 ttatccgggg aacagttaga agaaagtatt ccaggaatta gtgactcaga taaaattgtt
     1741 gtcttgtctg ctggtgggca attaatggga gatggcaaat ccgaattagt tagttataaa
     1801 caaaatcaca gacagtttat tgatgttaat actgattcca acgctatttc gattaaggat
     1861 ctaaaagagc ttttaaatag taaaagaaat gaattaaagc agataaaaga aactaattca
     1921 gtggaattag agtaaaggaa gattcattat gaaaaagatt tttggtgttt ctgttgggct
     1981 acttatgatg ttaacatttg gaaatgcagt atatgctgct gagaaagaag ttatttcaga
     2041 tccagagcag tacgcctcac gatactttgc tgattttgaa gaagaagata aagtacttgc
     2101 attgccagaa ggtggttttt taaaagggga agctaaaatt gtatctatgg ataatccaga
     2161 tataattcaa ggttcatata attcagaaac tgatcctgct tcagtttcag tgacagaagc
     2221 aaaaaaaatt gttgctgaaa aattcaaaaa tgatattgaa actactccaa ttatgcctcg
     2281 aggagcattt ccacccgatg ttggtactag cggacacctt cttgtactag caggaggagg
     2341 aagctataaa tcacagccat ttagtggtgc gggatggcgt tttggaggta tgggtgtaaa
     2401 accagctgat ggtacaggca actttttacg ttggaaatcc tacattgacg atggaagagt
     2461 aggaaatgta aatcaagcca acgcaactaa atctggttct attcaaggaa tggcattgta
     2521 tgctcgtgaa ggattcaaat actttgcacc aaacgggcct ggttggacaa tgaatatagg
     2581 tatttattac acttataatc ctgttgcagg tacgtattat gttgttcaaa atatttaata
     2641 aaacagcaca aagagatttc tttgtgctgt tttattattt ttagttgttg acaaacacta
     2701 aaatataaaa tataatcaac acataaacga acgtatgttc ggcaagaaag caggggattt
     2761 ttatggatga gatggtttta agtactcaga aatggctaaa caagaaatac agtaatgtta
     2821 cagggtttga taaggttcca gaaaatgggc gaacagggtg gcctacaatc tatggactta
     2881 ttgaaggact acaagtagaa cttggtatta ctaatctagt agctaatttt ggacctacca
     2941 ctgaaaaaat gtacgataat caggttactc ctaaatgggg caaaaatcta cctaaaaata
     3001 ttgtatttct tattcaaggt gccttttggt gtaaggggat taatccaggt ggcttcgatg
     3061 gggtatatac tccatattta gatactgctg taaaagagtt gcagactgat gcagggttta
     3121 atggaagcgc tgtaactgga gttttggatg cgaaatgggc caaagcgtta tttgatatga
     3181 gtgcttttgt tcttgttccc ggcggagata gtaaaattag aaaaatgcag caatggttaa
     3241 atgtaaatta tttagaatac acgggaataa tgccttgtga tggtatttat caaagaaatt
     3301 caaatcaagc attaattttt gcattacaag ctgaactagg atattctccc agtgaagcaa
     3361 cagggtctta tggtaatggg acaactagta aaacttatcc tgtgtctgaa ggtaactcgg
     3421 gaaactatgt aagaataatt caatatggat tatatgttaa tggttatttt gaagacggta
     3481 cattcgatgg tattttcact aaatatatgg gtttagaagt actggcattc cgaaaattta
     3541 tggtattgcc tgaatatact gaaatagcag atgttgttgt aataaaagga ctattgtcta
     3601 gcgctggaga tattagaaga tctgcagatg gcgcagatac ttctacgcaa ttaactagat
     3661 ctcaaatcca aacattggtt gataatgata taaaaatagt tggtagatat cttactggta
     3721 ctgttggaat tggaaaagat gaacgaaata agtatctaac tacagaggaa ttgaataata
     3781 ttttttctaa aaatttatct gtgtttccta tctatcaaga tggaggtgca gcattagctt
     3841 attttactta tgatcgtggc ttatcagatg gtaagaaggc tatagatgca gcaaaaaatc
     3901 taaatatacc attaacaaca gttatttatt ttgctgttga tctagacatg ttaggggaag
     3961 aaattcttgc atatgctgga gaatatttta agggggtatc tgctgtaatg tcatatagcg
     4021 ggtatcaaac gggtgtttac ggaacaagaa atgtttgttc tcaaataatc aataacgggt
     4081 atgcttcgtt tgcatttgtt tcagatatgt cgactggtta tagtgggaat ttaggttttc
     4141 ctatgcctag acaatggtct tttgatcaat ttgttgaatt taccatagga agtggcaacg
     4201 gagctgttgg tatcgattta atcgctacat cgggtagaga tagcggattt aatgagttat
     4261 caaatgatac taataatgat aattacattg ccaaatacaa tcagaaggtt ataaatcaga
     4321 tggtacgtgc gtatcaatat ctatcacaag ctggtcttga taatccttgg aaccctcatt
     4381 tgacatttta tagatatgta aattattctg gtttgtcatg ggacatcatt tcttctcctg
     4441 taacagagca tgatagaaaa atttatgatg aatataggaa taaattaact aatagcgagg
     4501 gcctgtataa ttattttata gatcctaata ctggttcaat aataggtttg cctcacttaa
     4561 ttgtgacgtt acaatcacag atgtttctag ccgacacaat caatgatagt gtttctgact
     4621 ttgcaggttg gttaggagat ttaatgactt gctggggtga agttaagaaa ttaggaatac
     4681 aaatggagca aggtgtattt gcattagtag gtacggctgc aggtggaaca tttagtctag
     4741 aagatttata ccaagatgtt gacgcggtaa atgtttatac cttagcaaaa agaaattctg
     4801 attcaacagc tttatcggct ttaaaaacgt actattgtga taagggtgct attgatagat
     4861 ttaatcaatt tgttaagaac agatttggtt cttcagataa aatcgcttca gtaacaaata
     4921 gtttattaga aaatgacgaa acagttgata tcttaggtgt agggataatt tcatttttca
     4981 ttgataaaat agttaataat tcacttggga cagattttac ttatcttgat tttattggag
     5041 atgaggggaa agttgcaaat ggattttctg aaaaaattat tcaatttcaa gaaaatagaa
     5101 gtaaacttaa ttaagaatcg tttactattt aaacagtgtc cttttccttt ttaaatatat
     5161 acgataaaat aagtatatga attgtaaaag gacatgatga ttatggaaga aatagaatgg
     5221 aataatctga ctcaaaataa taaaaataat tctggatgtg gaattatctt tatgtttgtt
     5281 atagttttgg gaatagtagt acttttaata actgtcaaga tagtagatta ctttgcgaat
     5341 actcctgaag aacaaataca gagaaaactt cctgagtttg aaagagtacc accttatgat
     5401 ccaagagata attcatttca agtaaaaaaa ttaaaagagt ctccaaatga aatgtggttt
     5461 gaagttacag ttgaatcaag cgacttcaaa gaaaaaaaga aagaatacaa ttatattgta
     5521 aaggcaaaag tatcaaaaag atttattttt aaccctaaat tagaaatata tagtgttgta
     5581 atagaataat agtgttaaaa aaattgtaaa agttaaagaa gtcaaattct gatgtgaaaa
     5641 ataaaatata ccatggaaga cataaaagaa aaactggata actagtttat ttaatggcag
     5701 accgttaagg cctgccattt ttatgtattt tagcggaata aatataatca tgcgggagtg
     5761 taaacttact gtttcaaaaa gtatactttt tgacatgcct tgattttctt tgtttatgta
     5821 tggcataata accacataat acaatttgaa acgtttctga tttaatctga ttgttttgaa
     5881 acaattttag tgaaaaaagg gtagataaaa acgatgagaa aattttttta tatccgggtc
     5941 agtagttatg atcaatcgat cgaacgacaa ctgatttccg cgaaggaatt aggaatccct
     6001 gaagaatata tatttgtaga aaaagccagc ggcaaagatt ttaaacgccc tgaatatcaa
     6061 ttaatgaaac ggatgtttcg aaaaggagat ttactctatc tacaatcatt agatagacta
     6121 ggcagaaata aacaaatgat tttagaagaa tggcgagaat taactaagga aaagcagatt
     6181 gatatttctg tgttagacat gcctctgtta gatactgcta gttacaagca actagatggg
     6241 ttagagacgc ttatctctga cttaattctg caattactca gctatatggc cgaagacgag
     6301 cggaaacgaa ttaatagccg gcaaagagaa ggcgtagaag ctgcgaaaca aaagggtgta
     6361 aaattcggca gaaagaaaat tgctttacct cctgaatttc ctgcgatcta ttctgactgg
     6421 aaagcaggaa agataactgc agtctatgct atggatcaat tgggcatgaa acgaaatacc
     6481 ttttatcgac gggtgaaaga atatgaacat accttgcacc acttttcttg atagatataa
     6541 cagctattag acgggaaggg agagtttgaa aatggactta acgaatcaaa gggtcgtttt
     6601 tactggaacg ctgcagcaaa tgacacgaac ccaagccatc ggtttggttc actctttaaa
     6661 cggccattgt caatcaagtg ttacttcaaa aacaaatttc ctggtttgtg gtcagtattc
     6721 taagtcgttg tttgatgatt ctctctatac aaaaaaggaa caaaccgcgc gggacttgat
     6781 tgcgttaggt catgagatta ccattttatc agaagtcgaa ttttatgcgt taattagtca
     6841 acagttgaaa gaatggcaac ggtatttata atcgttagta aaatttacaa ggaggaaaat
     6901 ttttatgaag cgaaaatctt atgatcgttg gggcattgaa ataactagtt ttaatcgaaa
     6961 tattgctttt cacgtggagt gtgactgtgg ggaattagca aaaagtgttt atcaaaaaaa
     7021 ttacttttac tgtcctaaat gccatcgccg atacggggta catggaacat agaaacgaaa
     7081 tagtgcggta gaaaagcgaa tgaatgtagt atatttctac aaatcattcg ctttctttat
     7141 tttataaata gttggtcgag agatcccgtt ttcttttgca atctctgcaa ctggaatgcc
     7201 ttgctttaac atgtccacca cggttttaaa aactaagcgt ttttgcggat ctgttgagtt
     7261 aatagaatac tcgattggtc gtcccttata tcggccggct ttttgagcca ataatactcc
     7321 ttgccgctga cgttcacgaa ttttctcacg ctcgttttgt gcaatataac ttagcagtga
     7381 taaaaacata tcgaacatag cggtatccag gagttcattt ccagtattaa aatctaaaaa
     7441 ctgtgcatct aatattttta acgctacttt tttctgtttc ataaaagcaa ctgtattttt
     7501 tatatcttca taatctcttc ctaaacgatc tagtgat
//
LOCUS       DAZKLM010000113         7282 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3764
            SAMEA115706913-rid22582563.denovo.118, whole genome shotgun
            sequence.
ACCESSION   DAZKLM010000113 DAZKLM010000000
VERSION     DAZKLM010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706913
            Sequence Read Archive: ERR13196647
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7282)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7282)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287823.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287823.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,004
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,849
            CDSs (with protein)               :: 2,849
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 41 of 96
            Pseudo Genes (incomplete)         :: 52 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 15 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7282
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706913-rid22582563.denovo.118"
                     /isolate="VRE3764"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            138..1292
                     /locus_tag="ACG842_002459"
     CDS             138..1292
                     /locus_tag="ACG842_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C47 family peptidase"
                     /protein_id="HFQ0248959.1"
                     /translation="MIILKPKKKIYLGLLFSLMIGIFSIGITNTYASENSNFNINSQP
                     IEEQAINFAKTDFEHSITSILLMENNTETDINYTLGSPFKMLDGENTANSSIFFPVFS
                     DADNTIKYVYTVKKNTNGEYSGSISQFLAKELQNLVDSNITDTTLFFQDNSIYYSDNG
                     QIKLLWKSPLSTEKNGVSSLTEKNDLISVNIKNKIKNFSKKQNFPAVKRVKSVSSGDN
                     FIMINWRIAETQTSFPWCAAYVEAAILCNKTDVRATSARAIMHYTYPNLNNSQIESKS
                     LNQDQIIKYANYCGVYPKKIARILNLSEVQTQLRKGNAIYMGTAGYGNYSNSRHALAL
                     FGWVNQGGYQTYYVWNPWYNYPTVISANAGPVTIPVPGGGFTWDNTITNW"
     gene            1265..1708
                     /locus_tag="ACG842_002460"
     CDS             1265..1708
                     /locus_tag="ACG842_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0248960.1"
                     /translation="MGQYHYKLVNKLIALVMGLVLVLFLTSCSKKQEIQKTDLIPFLG
                     TWQTKDHSERLVITNLNTSKNIEQYTILVKENNHKIQIIDKNLNINEQVFIYKATNNL
                     LTLRSNKKQLDYTFSLDSPKKLVFILTTNDGTEGTAKPIEFFKAD"
     gene            2842..3810
                     /locus_tag="ACG842_002461"
     CDS             2842..3810
                     /locus_tag="ACG842_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFQ0248961.1"
                     /translation="MANEIVKYHHELNTIPLRKFTSVEMNLFFSIISRMRDVGDKKVQ
                     FTFEQLKDLSNYKATANVRFIDDLETTYDKLMDLRFGRRSADGLQRERFVLFNQFKID
                     GKADIPFAEIQVHEKALPLLNNLEEWVRYSLQQFNELESSYSKTMFRLLKQYRTKGIA
                     NFSKEDFHELLDIPKSYRESDINKKIIKPIRQELTAIFKGLTIQKRYGKGRGKPVIGY
                     QFTFKPEIKKSDDFNKHAPTKEEQNKALELANQQVQSEKEPTSLQEKIKEQKRKLELL
                     QQLAELERKQNTPSKKINKQIADNNSDITPEFKENLLDNLQNLFKE"
     gene            3820..4308
                     /locus_tag="ACG842_002462"
     CDS             3820..4308
                     /locus_tag="ACG842_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HFQ0248962.1"
                     /translation="MTDKTIKELADILGVSKDKVKYQLRKLPSNFTYKKGNITYLKKE
                     AVTTITKVLSGNESGNLPSELPDSLPTDYLLKEIETKNNHIEEKDRQIEKLQIALETQ
                     QKLLDQQQQLTLQANQQIKKLHEQLQLTYAEESSEKEPLSVTEETQKGQNKEKKWWQF
                     WQ"
     gene            4604..4885
                     /locus_tag="ACG842_002463"
     CDS             4604..4885
                     /locus_tag="ACG842_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0248963.1"
                     /translation="MDILVRNIDPKVISEIDRRCQELTTKTGKKWSRNAYLKVLIEND
                     FDQALRHYKKDQFDLLLEKFIDIQAKNTETLNDYVHTNNQLIETLIGGI"
     gene            4887..5303
                     /locus_tag="ACG842_002464"
     CDS             4887..5303
                     /locus_tag="ACG842_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0248964.1"
                     /translation="MRKTSVQKHVTLYPSHIDVLDSIIEKNPDINNYSEAIRFLCMSY
                     EPNTNTTTTSNQAKLNAMGKEISIMSEIIAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     ENQLNPSRRNQPKNRQKETNQTFTSRNFDPTNFLKR"
     gene            complement(6058..7206)
                     /gene="tnpB"
                     /locus_tag="ACG842_002465"
     CDS             complement(6058..7206)
                     /gene="tnpB"
                     /locus_tag="ACG842_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family element RNA-guided
                     endonuclease TnpB"
                     /protein_id="HFQ0248965.1"
                     /translation="MEQLKAYKFRIYPTEEQEIFFAKSFGCVRKVYNLMLDDRKKAYE
                     EVKNDSSKKMTFPTPAKYKKEFPFLKEIDSLALANAQLNLDKAYKNFFRDKSVGFPRF
                     KSKKNPVQSYTTNNQNGTVALIDSKFIKVPKLKSLVRIKLHRQPKGIIKSATISRHSS
                     GKYYISLLCKEEIIELPKTNSTIGIDLGIMDFAILSDGQKIDNNKFTSKMEKKLKREQ
                     RKLSRRALLAKNKGTNLFEAKNYQKQKRKVARLHEKVMNQRTDFLNKLSTEMIKNHDI
                     ICIEDLNTKGMLRNHKLAKSISDVSWSKFVTKLQYKADWYGRKIIKVDKWFPSSQICS
                     ECGHKDGKKSLEIREWTCPVCHTHHDRDINASINILTEGLRLHSMGLA"
BASE COUNT         2586 a         1266 c         1185 g         2245 t
ORIGIN      
        1 acaaaataaa aaggcaatac gccttagttg tcggtcttcg agttaccaac ggtaaccctc
       61 ataccgaacg gcattcatca cccacctata gaggatgggc gacttctgcc taattctgtt
      121 aaatttaaca caaaggcgtg attattttga aacccaaaaa gaaaatttat ctaggtctcc
      181 tcttttcctt gatgattgga atttttagta taggaattac aaatacctat gcttcagaaa
      241 attctaattt caatatcaac tcccaaccaa ttgaagaaca agcaattaat tttgcgaaaa
      301 ctgattttga acactccata acttcaattc ttttaatgga aaataacacc gaaactgata
      361 ttaactatac tcttggttcg ccatttaaaa tgctcgatgg agaaaatacg gcaaatagtt
      421 ctattttctt tccagtattt tcagacgctg ataatacgat taaatatgtt tataccgtca
      481 aaaaaaatac aaatggagaa tattcaggaa gtatttcaca atttttagca aaagaacttc
      541 aaaatctagt agatagtaat ataacagata caaccttatt ttttcaagat aatagcattt
      601 attattccga taatggacaa attaaactac tttggaaatc acctttaagt acagaaaaaa
      661 atggtgtttc ttcccttaca gaaaaaaatg atcttatatc cgtaaatata aaaaataaaa
      721 ttaaaaattt ttctaaaaaa caaaattttc ctgcggttaa acgtgtaaaa agtgtctcct
      781 ccggagataa ctttattatg attaattggc gtatagcaga aacccaaaca agttttccgt
      841 ggtgcgctgc ttacgtagaa gctgctatct tatgtaacaa aacagatgta cgtgctactt
      901 cagctagagc cattatgcat tatacctatc ctaatttaaa caacagtcaa attgaatcaa
      961 aaagtcttaa tcaagatcaa ataataaaat atgcaaatta ctgtggtgtt tatcctaaaa
     1021 aaatagcacg aatactaaac ctttcagagg tccaaacaca actaagaaaa ggaaatgcca
     1081 tttatatggg aactgctggt tatggaaatt atagtaacag ccgtcatgca cttgcattat
     1141 ttgggtgggt caatcaaggt ggttatcaaa cttactatgt gtggaatcct tggtataact
     1201 acccaactgt aataagtgca aatgcaggac cagttacaat tcctgtacct ggaggtggtt
     1261 ttacttggga caataccatt acaaattggt aaataaactc attgctttag ttatgggtct
     1321 agtgttagtt cttttcttaa ctagttgttc aaaaaaacaa gaaatacaaa agactgatct
     1381 gattcctttt ctaggaactt ggcaaactaa agatcattca gaacgtctag taatcactaa
     1441 tttaaatact tcaaaaaata tagaacaata taccatctta gtaaaagaga acaatcataa
     1501 aattcaaatc attgataaaa atttaaatat aaatgaacaa gttttcattt acaaagcaac
     1561 aaataattta ctaacactgc gctcgaataa aaaacaatta gattacactt ttagtttgga
     1621 ttctccaaaa aagctcgtat ttattttgac aacgaatgat gggacagaag ggacagctaa
     1681 accaattgaa ttttttaaag ctgattgatg aatagttgtt tgatattaca aaacaataat
     1741 ttcaccgaag gtgaaacaag cgaaacttta caaaaagtgg ctagcatttg ctagcctttt
     1801 ttgtattgtg gagcgtgggt tttgaggggg attcaagggg gagcttgcgc ccctttgaca
     1861 agcggttatt tgcaccaaat acgtaggtat ttggctagca aatatagagc ttgccaagcc
     1921 ctttagtgtg ttttgtagaa agggacctta tagtcacttt cacattacaa acgatacgtc
     1981 ctatggacgt atcaaaaggg tgggacttat gagcaaatta aagcaaaatt tggctagcaa
     2041 tccttctaaa aaatacacct attgatcaga gtcaaaataa atcagttttc gagtgagcga
     2101 atccaaattt gcaactctga agctttgcaa atgagtgtgc cggcttccgt aaaagtctag
     2161 gtaacattag tcgatatata catagggctc taatttgcgt tttaaggcgt tttaaacgtt
     2221 ctggtatgta attataccaa agcgacttta aaacgcctta aaacgaattt tagaggttct
     2281 gtagaacgtt ttagggttgt tagggcttgc cctgaccgcc tgtaaggcgc tttaaaaaca
     2341 agataaggga ttttatctag acttgtctta aaacgccgta gatgagcgct cagagcttct
     2401 aaaaggactc cttacgaact gtttttaagc aaaggagcct ttaggcgact gcgcagttta
     2461 atgtgagagc gtagcgatca cacctttaaa gcgaccgtag ggagcagatt cccagtttag
     2521 gaaatttcgc gaacctgttt ggagaaaaaa agtaggaaaa ttttaggcgg gaaattttgc
     2581 ccccctcttt tataatctct tttataacct ctttttaaac ctctttaccg tcggctgtat
     2641 ccttactctc ccaaggtgtg cagaatcttt taggggtctt tttgtctgtt tttaggggtc
     2701 tttttgtctg tttttagggg tctttttgtc tgtttttagg ggtctttttg tctgttttta
     2761 ggggtcttta attagggggt tgacccccat tagaaagctc tttataattt tgttaagtag
     2821 ttaatcttga aggaggattt tatggcaaat gaaattgtga aataccacca tgaattaaat
     2881 acgattcctt tacgcaaatt tacgtcagta gaaatgaatt tattcttctc tataatatct
     2941 agaatgcgtg atgtaggcga taaaaaagta caatttacat tcgaacaatt aaaagattta
     3001 agcaattata aagcaactgc aaatgttcga ttcatagatg atttagaaac aacgtatgac
     3061 aaattaatgg atttacgttt tggtagaaga agtgctgacg gtttgcaacg tgaacgattt
     3121 gttttattta atcaatttaa aatcgatgga aaagctgata ttccttttgc tgaaatacaa
     3181 gtccatgaga aagccttacc cttacttaat aatcttgaag aatgggttcg atattctctc
     3241 caacaattca atgaattaga aagcagttat tccaaaacca tgtttagatt attaaaacaa
     3301 tacagaacca aaggaattgc caatttttca aaagaagact ttcatgaatt acttgatatc
     3361 cctaaaagtt atagagaaag tgatataaat aaaaaaatta taaagcctat tcgccaagaa
     3421 ctaaccgcaa tcttcaaagg tttgacaatc caaaagagat acggaaaagg acggggaaag
     3481 cctgtcattg gctatcaatt tacatttaaa cctgagataa aaaaatcaga cgactttaac
     3541 aaacatgctc caaccaaaga agaacaaaat aaagctttgg aactagctaa tcaacaggtt
     3601 caatctgaaa aagaacctac ttcattacaa gaaaaaatca aagaacaaaa acgaaaatta
     3661 gaacttttac aacagttagc agaattagaa agaaaacaaa ataccccaag taagaaaatt
     3721 aacaaacaaa tagcagataa caattccgat attaccccag aatttaaaga aaacttacta
     3781 gataatctcc aaaacttatt taaagaataa ggggttttta tgacagataa aactatcaaa
     3841 gaattagcag atattctagg tgtttcaaaa gataaagtga agtatcaact gaggaaatta
     3901 cctagtaatt ttacctacaa aaaaggaaat attacctacc tgaaaaaaga agcagtaaca
     3961 acaattacaa aggttttatc aggtaatgaa tcgggtaact tacctagtga attacccgat
     4021 tcattaccta ccgattacct actcaaggag attgaaacta agaataatca tattgaagaa
     4081 aaagataggc aaattgaaaa acttcaaata gctttagaaa cgcagcaaaa attactcgac
     4141 cagcaacaac aactcaccct acaagccaat caacaaatca aaaaacttca cgaacaactc
     4201 cagctaacct atgcagaaga atcttcagaa aaagaacccc tttctgttac tgaagaaact
     4261 caaaaaggac aaaacaagga gaaaaaatgg tggcaattct ggcaataacc caacaatttt
     4321 tattcataaa aattgttggg ttactttctt tttttgcttt tacaagcata gcgacatgat
     4381 ctcacgtcgc tattttttaa agagcataag gaaacgcggt atcactttct tacgaaaggt
     4441 gctaggtagg ctgtgcctac cacgtttcta agaaaaaaat ttgctatcac tttttcgtca
     4501 ttgtggatag cttatgtata gtgtttcggc aaaaaacagc tatacaaata ctatatttgt
     4561 atgatataca taaattatac acaaatgcga aaggaattct tctatggaca ttttagttcg
     4621 gaatattgat ccaaaagtta tttcagaaat agatagacgt tgccaagaac tcacaacaaa
     4681 aactggcaaa aaatggagcc gtaatgcgta cctaaaagtt ctaatcgaaa acgattttga
     4741 ccaggcactt aggcattaca aaaaagatca attcgattta ttactcgaaa aatttatcga
     4801 tatacaagca aaaaatactg aaacattgaa cgactatgtt catacaaaca accaactcat
     4861 tgaaacactt atcggaggaa tataacatga gaaaaacttc tgttcaaaaa cacgttaccc
     4921 tttatcccag tcacattgac gttttagatt ccatcatcga aaaaaatcca gatatcaata
     4981 actactcgga agccattcgt tttctatgta tgagttatga acctaatact aatacgacaa
     5041 ctaccagcaa ccaagcaaaa ctaaatgcga tgggaaaaga aatttctatt atgtccgaaa
     5101 tcattgcaga atttgctgat ttatatctaa aagatactgg tgttcttatc ggtacaaatt
     5161 ctaaagtata tcaagaagtg aaagaaaaaa ttgaaaatca actcaatccc tcccgacgaa
     5221 atcaacccaa aaatcgtcaa aaagaaacca atcaaacatt cacttctaga aattttgacc
     5281 caacaaactt tctaaaaaga tgaaaaagaa accgaaaatt ttttctattt ctcagtttct
     5341 tttttagggg gcagtcttcc agcaagattt gcaagttacc ggtaacttgc acaaaaaaag
     5401 aaaaatccgc ttgtgcgaat ccttcttttt gcattttggg gcgctgcccc aatctccaaa
     5461 tatcggttat ttaatagcgt ggtaactcaa aaaaatagaa cgtactctga aataaaaaaa
     5521 gactgctcta acgagcagcc aataaaaaat aatgagcgtt tattcttgaa aaagcagcgc
     5581 gcttatgaag cgctgccaaa aatttaccat attttatgaa aaataacaag tttctaaaaa
     5641 ggaatcggtt aagattcaca aagaatcgta aacagacaat agcggaaaat caagcttccg
     5701 aacgtacctt ttagctagac caatgaaaaa tcacaaagtg agcccctgta tcggttgata
     5761 caagggctac ttggcatact cgacattatg tctactagct cgataaataa cgattatgtt
     5821 cccaaaacaa tattttatga actataacaa aaatttgtta tcgcatattg tatattttaa
     5881 ttataggtgt atatcacgtg aactactcac cacttagcta aacctgacgg ttcttaacgc
     5941 ttgaagtggg agcttcttgg gaatagagca tacttgatag cgtatttctt tactaacagc
     6001 ggtgtctctt atttaccaag ctatccccgt agttcctacg gttcttgatg attggatcta
     6061 agccaatccc atagaatgta gtcttagacc ttcggtcaga atattgatac tagcattgat
     6121 atcacgatca tgatgagtat gacaaacagg acaagtccat tctcgaattt caagagattt
     6181 cttgccatct ttatgtccgc actctgaaca aatctgacta gacggaaacc atttatctac
     6241 tttgatgatt tttcgtccat accagtcagc cttgtattgt aatttagtca caaactttga
     6301 ccaagaaaca tcagaaatac ttttagctaa tttatgattt cgcaacatac cttttgtgtt
     6361 taagtcttca atacagataa tatcgtgatt tttgatcatt tctgtactca acttattcag
     6421 aaaatcagta cgttgattca ttactttttc atgcaatcta gctactttgc gtttttgttt
     6481 ttgataattt ttagcttcaa atagattggt acctttattt ttagccaata aagcacgcct
     6541 agacaatttg cgttgttcac gctttagttt cttttccatt ttggacgtga atttattatt
     6601 atcaattttt tgtccatcag aaagaatcgc aaagtccata atacctaagt caataccaat
     6661 tgtggaatta gttttaggta attcgataat ttcttcttta cacaacaaag aaatatagta
     6721 tttaccgcta gaatgacgtg atattgtagc agatttgata atcccttttg gttgtctatg
     6781 aagcttaatt cttactaatg acttcaattt aggaactttg atgaatttac tatcaatcaa
     6841 agcaactgta ccattttgat tattcgttgt ataactctga acaggatttt tcttactttt
     6901 gaaacgtgga aatccaacgg atttatcccg aaaaaaattc ttgtatgctt tatctaaatt
     6961 aagttgggca ttggctaaag caaggctatc aatttctttc aaaaacggaa attctttctt
     7021 gtatttagct ggtgtcggaa aagtcatttt tttagaagaa tcatttttaa cttcttcata
     7081 cgcctttttt cggtcatcaa gcattagatt gtagacctta cggacacaac caaaagattt
     7141 ggcaaagaag atttcttgtt cctctgttgg atagattctg aatttgtatg cttttagttg
     7201 ttccataaag ttcacctact ttctatttat gattgtttcc ttgattttgg atatacttct
     7261 taatcacatc aattggtgcg cc
//
LOCUS       DAZKLM010000114         7217 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3764
            SAMEA115706913-rid22582563.denovo.119, whole genome shotgun
            sequence.
ACCESSION   DAZKLM010000114 DAZKLM010000000
VERSION     DAZKLM010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706913
            Sequence Read Archive: ERR13196647
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7217)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7217)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287823.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287823.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,004
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,849
            CDSs (with protein)               :: 2,849
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 41 of 96
            Pseudo Genes (incomplete)         :: 52 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 15 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7217
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706913-rid22582563.denovo.119"
                     /isolate="VRE3764"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(229..906)
                     /gene="phoU"
                     /locus_tag="ACG842_002466"
     CDS             complement(229..906)
                     /gene="phoU"
                     /locus_tag="ACG842_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010723432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0030643 - intracellular phosphate ion
                     homeostasis [Evidence IEA];
                     GO_process: GO:0045936 - negative regulation of phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HFQ0248966.1"
                     /translation="MLRTQFEEELLNLHNQFYEMGMMVSSAVHKSVRAYIKHDKILAQ
                     EVIENDININNMETRLEKKSFEMIALQQPVTTDLRMIITVMKASSDLERMADHAVSVA
                     KSTIRVKGQTRIPEIEKEISDMSDYVKKMVDNVLVAYVKTDEKDARTIANMDQRVNDY
                     FNRIYNATIKNMQENPETVISGTDYLNVASYLERIGDYVTNICEWIVYLATGKITELN
                     TNHNEIF"
     gene            complement(919..1677)
                     /gene="pstB"
                     /locus_tag="ACG842_002467"
     CDS             complement(919..1677)
                     /gene="pstB"
                     /locus_tag="ACG842_002467"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HFQ0248967.1"
                     /translation="MTKEIISSKDLHLYYGKKEALKGIDLSFNQGEITAMIGPSGCGK
                     STYLRSLNRMNDLIPNVTITGSVLYKDKDIYGPKTDVVELRKEIGMVFQQPNPFPFSI
                     YENVIYGLKLKGEKDKKVLDQVVEESLKAASVWDDVKDKLHKSALSLSGGQQQRVCIA
                     RVLAVNPEIILLDEPTSALDPVSTGKIESMLLELRERYTMIIVTHNMSQASRISDKTA
                     FFLDGHLIEYNDTKKIFMNPEKQETEDYISGRFG"
     gene            complement(1693..2499)
                     /gene="pstB"
                     /locus_tag="ACG842_002468"
     CDS             complement(1693..2499)
                     /gene="pstB"
                     /locus_tag="ACG842_002468"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002408906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HFQ0248968.1"
                     /translation="MKKYNWNETNVITMPKEKEVALYTDDIHVWYGDNEAIKGVDLEF
                     EKNKITALIGPSGCGKSTYLRSLNRMNDGIANTKITGKIMYQGVDINAPQVDVYEMRK
                     RIGMVFQRPNPFSKSIYDNITFALKQHGEKNKKKLDEIVETSLKQAALWDQVKDSLDK
                     SALALSGGQQQRLCIARAIAMKPDILLLDEPASALDPISTGTVEETLVNLKEDYTIII
                     VTHNMQQAARISDYTAFFYLGKVMEYDKTNKIFTRPKIQATEDYVSGHFG"
     gene            complement(2514..3398)
                     /gene="pstA"
                     /locus_tag="ACG842_002469"
     CDS             complement(2514..3398)
                     /gene="pstA"
                     /locus_tag="ACG842_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010764435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HFQ0248969.1"
                     /translation="MNAKRSDKIATGVLYIVSGIIVLILAALLLYILVRGIPHISWSF
                     LTEPSKAYQEGGGIGIQLFNSIYLLLITMIISFPISLGAGIYLSEYAGKNWLTDVIRT
                     SIEILSSLPSVVVGLFGFLVFVIQFQYGFSIISGALALTVFNLPLLTRTVEESLQAVH
                     YTQREAGLALGLSRWETVMKVVVPEATPGILTGVILSSGRIFGEAAALIYTAGQSAPA
                     LDFTNWNPLSVSSPISIFRQAETLAVHIWKINTEGTMPDGVSVSAGASAVLIIAVLLF
                     NFGARAIGKRLYRKMTSA"
     gene            complement(3398..4318)
                     /gene="pstC"
                     /locus_tag="ACG842_002470"
     CDS             complement(3398..4318)
                     /gene="pstC"
                     /locus_tag="ACG842_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HFQ0248970.1"
                     /translation="MEDVKKVLLTKSKRSKMEQRGKFISFLCIALIVLVVLSIFYFVA
                     SKGLATFFTDKINVFDFLFGTDWNPSQIGANGKPMVGALPMITGSFIVTFLSAIVATP
                     FAIGAAVFMTEISPKNGTKILQPVIELLVGIPSVVYGFIGLSVIVPFVRSIFGGTGFG
                     ILAGTFVLFVMILPTVTTMTVDALKAVPRHYREASLALGATRWQTIYKVVLRAAVPGI
                     LTAVVFGMARAFGEALAIQMVIGNAALMPTGLTTPASTLTSILTMGIGNTIMGTVENN
                     VLWSLALILLLMSLFFNIVIRMIGKKGELK"
     gene            complement(4433..5290)
                     /locus_tag="ACG842_002471"
     CDS             complement(4433..5290)
                     /locus_tag="ACG842_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HFQ0248971.1"
                     /translation="MKKLKLFLPIIGLAILLSGCSKWIDRGESITAVGSSALQPLVET
                     VAETYQTENPGKFINVQGGGSGTGLSQVQSGAVDIGNSDLFAEEKSGIDASALVDHRV
                     AVVGITPIVNKDIGVKDISMENLKKLFTGEIKNWSEVGGKDQPVVILNRASGSGTRST
                     FEKWVLDGETAVQAQEQDSSGMVRQIVADTPGAISYVAFSYVSDDVDTLNVNGVEPTD
                     ENVTTNKWTIWAYEHMYTRDHPKKLTKEFLDYILSDEVQDNIVGELGYIPVSHMKVER
                     DWQGNIVKE"
     gene            complement(5627..7075)
                     /gene="cls"
                     /locus_tag="ACG842_002472"
     CDS             complement(5627..7075)
                     /gene="cls"
                     /locus_tag="ACG842_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HFQ0248972.1"
                     /translation="MKIIVDNFFTILLIMNILLSFIIVFRERKETAQTWAWLLVLMFI
                     PVVGFILYIFLGRGISKEKIFDLKIQGKIGKNLEIEEDKQALMRGLYPHPPTGQVDVK
                     QLIYMLTVFESTLYTTKNEITLFTDGREKFDALIEDIKQAEDHIHFQYYIYRSDALGE
                     EVRDALTEAARRGVKVRVLLDAWGSTQVSSSFFQNLKKAGGEVAFFFPLFVPYINPRI
                     NYRNHRKIVVIDGKIGYTGGFNVGNEYLGLVKKFGYWRDNHLRIYGEAVYTLQNRFLM
                     DWNSQHTKEVGYSPKFFPSIHTTGDIAVQIVTSGPDTEHEQIKMTYLKMISLAKREIL
                     IQTPYYIPDGSIHEALKLALLSGVKVHIQIPNKPDHLLVYWATYSFAAELIEYGARIE
                     TYENGFIHAKTMIIDGGIVSVGSANIDVRSFRLDFEVNTLVYDERMASRVRKAFFEDS
                     KISTHLTKEMYENRGIIIKMKEGLARLISPLL"
BASE COUNT         2093 a         1589 c         1255 g         2280 t
ORIGIN      
        1 ttgcattctt tatcctttct atgaaatgat gaaaagggtt tgtgacatgt tttgtcacaa
       61 accctttccc gaataaacgg tgttcaaaag cttgcatctg cggtaataag tccagccaaa
      121 cgaaaaatat gaggagctat tttttgtttg actgttctta ttacttgatg tagaacagct
      181 ttttcacaac ctcttttcat aaacaaacgg cataatcaca gcttcagttt agaaaatctc
      241 attgtggttc gtatttaact cagtgatttt ccctgtagcc aagtagacga tccattcaca
      301 gatgtttgtc acatagtctc cgatccgttc tagataactg gcaacattca agtagtctgt
      361 tccactgatg actgtctcag gattttcctg catatttttg attgttgcat tatagattcg
      421 attgaaatag tcattgactc tttgatccat attggcgatc gtacgtgcgt ctttttcgtc
      481 tgttttgaca taagcaacta ggacgttgtc caccattttt ttcacataat cggacatatc
      541 ggagatctct ttttcgattt ccggtattct cgtctgccct tttacgcgaa tcgtggattt
      601 agcaacagac accgcatgat cggccatacg ttctaaatcc gagctagctt tcatgaccgt
      661 aatgatcata cgcaaatcag ttgtaactgg ctgttgcaat gcgatcattt caaaactttt
      721 cttctccaag cgtgtttcca tgttattgat atttatatcg ttttcgataa cttcttgggc
      781 taagatttta tcgtgtttga tgtatgcacg aactgacttg tgcacggcgc tgctcaccat
      841 catccccatt tcgtagaact gattatggag attcagcaat tcttcttcaa attgtgtacg
      901 tagcattccg tttcctcctt atccaaatcg acctgaaata taatcttctg tctcttgttt
      961 ttctggattc atgaatatct tcttcgtatc gttgtattcg atcaagtgac catctaaaaa
     1021 gaatgccgtc ttatctgaaa tacgtgaagc ttgagacata ttatgggtca cgatgatcat
     1081 cgtataccgc tctcttaatt ctaacagcat actttcgatt ttcccagtag aaacaggatc
     1141 aagcgcactt gttggttcat ccaataagat gatttccgga ttaactgcca aaacacgtgc
     1201 gatacataca cgctgctgct gcccgccgga taaagataaa gcacttttat gcaatttgtc
     1261 tttcacatca tcccaaacag acgctgcctt caggctttct tcaactactt gatcaaggac
     1321 ttttttatct ttttcccctt ttaatttcaa tccatagatg acattttcat agatagaaaa
     1381 aggaaatggg tttggctgct ggaataccat gccgatttct tttcttagtt caacaacatc
     1441 tgtttttgga ccatagatgt ctttgtcttt atataaaaca cttccggtga tcgttacatt
     1501 cggaatcagg tcattcatcc ggttcaaaga acgcagataa gtggattttc cgcaaccaga
     1561 aggaccgatc atcgcagtga tttccccttg attgaaactt agatcgatcc cttttagtgc
     1621 ttctttcttt ccataataca gatgaagatc tttagatgaa ataatttctt ttgtcatgtt
     1681 gaggcctccg tcttaaccaa aatgaccgga cacataatct tcggttgcct gaatttttgg
     1741 tctggtaaat atcttgttag ttttgtcata ttccattact tttcctagat agaaaaaggc
     1801 agtatagtcg ctgatacgag ctgcttgttg catgttatgc gtcacgatga tgatcgtata
     1861 gtcttctttc aagtttacaa gtgtttcttc aaccgttcct gtcgaaatag ggtccaaagc
     1921 acttgccggc tcgtctagca gcaagatatc aggtttcatc gcgatcgcac gagcaatgca
     1981 taaacgttgc tgttgcccgc cagataaagc caaggcactt ttgtccaagc tgtcttttac
     2041 ttggtcccac aaggcagctt gtttcaagct tgtttcaacg atttcatcta atttcttttt
     2101 gtttttttcg ccgtgctgtt tcaaagcaaa tgtaatattg tcataaatcg acttgctgaa
     2161 cggattagga cgttgaaaaa ccatgccaat ccgtttgcgc atctcataaa catccacttg
     2221 aggcgcatta atatctacgc cttgatacat gattttccca gtgattttcg tattagcgat
     2281 cccatcattc attcgattca acgaacgaag atacgtagat tttccgcagc cggaaggacc
     2341 aatcagtgca gtgatcttat ttttttcaaa ttccagatcc actcctttga ttgcttcatt
     2401 gtcgccatac cagacatgta tatcatccgt atatagtgct acttctttct cttttggcat
     2461 ggtgatgaca tttgtttcat tccagttata ttttttcatt ttgcttcttc tccttaggct
     2521 gaagtcattt ttcggtataa tcgtttgcca atcgcacgag cgccgaagtt gaacagtaaa
     2581 acagcaatga tcaacacggc agaagcgcca gccgaaacag aaacaccatc aggcatcgtg
     2641 ccttctgtat tgatcttcca aatatggaca gctaatgttt cagcttgacg gaaaatgctg
     2701 atcgggcttg aaacacttaa tggattccag ttcgtaaaat ctaacgcagg tgcactttgt
     2761 ccagccgtat agatcaatgc agctgcttca ccaaaaattc ttccagaact taggatcaca
     2821 ccagtcaaaa tccctggtgt cgcttctgga accaccactt tcatcactgt ttcccagcgt
     2881 gacaaaccta aggccaatcc tgcttctcgt tgtgtgtaat ggaccgcttg taaagactcc
     2941 tcaactgttc tagtcaaaag aggcaagttg aatacagtca aagccaatgc accagaaata
     3001 atcgagaaac catactggaa ttgaataacg aaaaccaaga aaccgaataa accgacaacg
     3061 acagaaggca acgagctcaa gatttcaatc gacgtgcgaa tcacatctgt tagccagttt
     3121 tttccagcat attctgaaag atagataccc gcacctaaag aaataggaaa actgatgatc
     3181 atcgtgatca aaagcaaata aatcgagtta aatagctgga tcccgattcc accaccttct
     3241 tggtaggctt tggaaggttc ggtcaagaag gaccatgaaa tgtgcggtat tccccgaact
     3301 aagatataaa gtagtaaagc tgctaaaatc aatacgatga tccccgaaac tatatataaa
     3361 acacctgtag caattttatc tgagcgtttt gcgttcatta tttcagctct cctttcttcc
     3421 caatcatacg gatcacgata ttaaagaata atgacatcaa tagcaagatc agcgctaatg
     3481 accataacac attattttca actgttccca tgattgtatt tccgattccc attgttaaga
     3541 tggacgtcaa tgtagaagca ggagtagtaa gtcctgttgg catcaatgct gcattaccga
     3601 tgaccatttg gatagctaag gcttcaccaa acgcacgagc cataccaaaa acgactgctg
     3661 ttaaaatccc gggcacggct gctcgtagca cgactttgta gattgtttgc catcttgtgg
     3721 caccaagcgc taatgaagct tcccgatagt gccgtggtac tgcctttaat gcatcaacag
     3781 tcatcgttgt gacagtcggc aagatcataa cgaacaacac aaatgtacca gctaagatac
     3841 caaaccctgt cccgccaaaa atacttctga caaaaggcac gataactgat aaaccgataa
     3901 aaccataaac gacagaagga atacctacaa gcagctcgat gaccggctgt aagatcttcg
     3961 ttccgttttt aggagagatc tccgtcatga atactgctgc accaatggca aatggcgtgg
     4021 caacgatagc agataaaaag gtcacaataa acgaacctgt gatcataggc aatgccccta
     4081 ccattggttt cccatttgct ccgatttggc ttggattcca atctgtacca aacagaaaat
     4141 caaatacatt gatcttatca gtaaaaaagg tggccaatcc tttactagct acaaaataaa
     4201 aaatagataa tacaaccaac acgatcagcg cgatacataa aaaactgatg aattttccac
     4261 gctgttccat ttttgaacgt tttgattttg ttaataatac ttttttcaca tcttccacag
     4321 atgactcccc cttaatcagg tttaacatac tcactcagtg tacaaattga acatcaattt
     4381 ggttttaata acatgtaaaa gtttagctaa gtttgtaaat tttgtgtaaa gattactctt
     4441 taacgatatt tccttgccaa tcacgttcga ctttcatatg agaaacaggg atataaccta
     4501 gttctccaac gatgttatct tgtacttcat cagataagat atagtctaaa aattcttttg
     4561 tcagtttttt tggatgatct cttgtgtaca tatgctcata tgcccaaatc gtccatttgt
     4621 tcgttgtcac attttcatct gtcggttcta caccgttaac attcaacgta tctacatcat
     4681 cggaaacata agaaaaagca acatagctga ttgcaccggg tgtgtcggca acgatctggc
     4741 gcaccattcc gcttgaatct tgttcttgtg cctgaacagc tgtttctccg tccaataccc
     4801 atttttcaaa cgtgctacgg gttccgcttc ctgaagcgcg gttcaatata actactggct
     4861 gatcttttcc accgacttca ctccagtttt tgatctcgcc tgtaaatagt tttttcaggt
     4921 tttccatact gatgtctttt acgccaatat ccttgttcac aataggagtg atcccaacaa
     4981 ctgctactcg atgatcgact aatgctgagg catcgatccc cgatttttct tccgcaaaca
     5041 aatcagagtt tccgatgtcg actgctcctg actgtacttg agacaaacca gttccgcttc
     5101 cgcccccttg tacattgatg aattttccag gattttctgt ctggtaagtt tcagcaactg
     5161 tttctactaa aggctgtaaa gcagaagagc caacagccgt aatggattct cctcgatcaa
     5221 tccactttga acagcctgat aaaagaatgg ctaatcctat tattggtaag aataatttaa
     5281 gttttttcat tagaaatctc ctttaatcct aaagtatgtt tattttatca ttttcattac
     5341 ttgatgaaaa cgaatcaaaa tcattaaata acaaaataaa ttctcataat tgtaaaaaaa
     5401 actatctcat taaaagtttc tatgctatac tggctttgaa atgtgtctct tttaatagca
     5461 tatattagct tatttcttgc tttccccaaa aaacaagaga taccccaaag ttaatttcgc
     5521 tcatgcgaga cgctttctcc atttctgacg tactctcaga aatggccccg ttagaaaagg
     5581 acacgagatc tccatctgtt gatggttctc atgtcctttt tatgttttaa aggagcggcg
     5641 aaatcaatct tgctaatcct tctttcatct tgatgattat tcctcggttt tcatacattt
     5701 cttttgtgag atgcgtagaa attttcgagt cttcaaaaaa agcttttctt acacgtgaag
     5761 ccattcgttc atcataaacc agtgtattga cctcaaagtc caagcgaaat gaacgaacat
     5821 cgatatttgc tgagccaact gaaacaatgc caccatcgat aatcatcgtc tttgcatgaa
     5881 taaatccatt ctcatatgtt tcgatccttg ctccatactc gatcagctct gcagcgaaag
     5941 aatacgttgc ccagtaaacg agaagatgat ctggtttgtt aggtatttgg atatgaactt
     6001 ttacgcctga aagaagagcc aacttcagtg cttcatgaat cgatccatcc ggtatgtaat
     6061 aaggcgtttg gatcaatatc tcccgtttgg ctaacgaaat cattttcaaa taagtcattt
     6121 tgatttgttc atgttcggta tctggtccgc ttgtcacgat ttgaacagct atatcaccag
     6181 tagtgtgaat ggacgggaaa aatttgggag aatagcctac ttcttttgta tgctgagaat
     6241 tccagtccat caaaaaacgg ttttgcaagg tatacaccgc ttctccgtaa atacgcaaat
     6301 gattgtctcg ccaataacca aatttcttta ccaatcctag atattcattt ccaacattga
     6361 acccacctgt atatccgatt ttcccatcaa taactacgat tttacgatga ttgcgataat
     6421 tgatccgagg attaatatac ggaacaaata gaggaaagaa aaatgcgact tctccgcctg
     6481 cttttttcaa attctgaaaa aagctggaag aaacttgtgt ggaaccccat gcatcaagaa
     6541 ggactctgac ttttacccct cgtcttgccg cctctgtcaa tgcatctcga acttcttcac
     6601 ctaatgcatc actgcgataa atataatact ggaagtggat atggtcttct gcctgcttga
     6661 tatcttcaat cagcgcatcg aatttttcac gaccatctgt aaataaagtg atttcatttt
     6721 tagtcgtata gagcgtactt tcaaataccg tgagcatata gatcaattgt ttcacatcta
     6781 cttgtccagt aggcggatga ggataaagcc ctcgcatcaa tgcctgtttg tcttcttcaa
     6841 tttccaggtt cttaccgatt ttcccttgga tcttcaaatc aaagattttc tctttggaaa
     6901 tccctcttcc taaaaaaata tataaaataa atcctacaac tggaatgaac atcaaaacga
     6961 gcagccatgc ccatgtttga gcagtttcct ttctttctcg aaaaacgatg ataaaggaaa
     7021 gcaaaatatt cataatcaaa aggatcgtaa aaaaattgtc aacgataatt ttcacggctt
     7081 cacctcttct tctaccaact taagaatacc caagttgttt tttaaagtaa agggaaaaaa
     7141 gccgcttcct tctattctcc tagccaataa aaaaagaaag ctggaacgaa actgtccaac
     7201 tttctcattt tatttat
//
LOCUS       DAZKLM010000115         6921 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3764
            SAMEA115706913-rid22582563.denovo.120, whole genome shotgun
            sequence.
ACCESSION   DAZKLM010000115 DAZKLM010000000
VERSION     DAZKLM010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706913
            Sequence Read Archive: ERR13196647
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6921)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6921)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287823.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287823.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,004
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,849
            CDSs (with protein)               :: 2,849
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 41 of 96
            Pseudo Genes (incomplete)         :: 52 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 15 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6921
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706913-rid22582563.denovo.120"
                     /isolate="VRE3764"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..182)
                     /locus_tag="ACG842_002473"
     CDS             complement(<1..182)
                     /locus_tag="ACG842_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFQ0248973.1"
                     /translation="MTSLKTLLFGTTLAAGAAFFMGTTAHADEAYTVQSGDTLSTISQ
                     KYVGDNSLINAIAESNS"
     gene            517..951
                     /locus_tag="ACG842_002474"
     CDS             517..951
                     /locus_tag="ACG842_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HFQ0248974.1"
                     /translation="MSKRYQNIMVAVDGSRQSIKAFAEALDLAKDNEAHLFIVSIINK
                     VELTHSAYAFSKIYADEKQRTEVAMLKKINDAKEYGIAEIHAIVETGDPRNLIANVIP
                     GQENIDLIVMGATGKGAIQQALVGSTASYVVSHAPCSVLVVK"
     gene            974..1294
                     /locus_tag="ACG842_002475"
     CDS             974..1294
                     /locus_tag="ACG842_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0248975.1"
                     /translation="MVRPQEVRAPKEKIEILAIIEDGTQTKKGYSIALVKWKGKKGVA
                     LRWDGDNQQDKGFPVTANGYHPAWFVLPDKFMELYSYDYARAAASIKALDKLTEEQNE
                     MEEK"
     gene            1435..1626
                     /locus_tag="ACG842_002476"
     CDS             1435..1626
                     /locus_tag="ACG842_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0248976.1"
                     /translation="MEAGKVLQEVKALCADKKYEEAKSLIEKNKENLEEKYPIAQEFI
                     ELKQGTILHRFKHFFGITE"
     gene            complement(1697..1954)
                     /locus_tag="ACG842_002477"
     CDS             complement(1697..1954)
                     /locus_tag="ACG842_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake protein"
                     /protein_id="HFQ0248977.1"
                     /translation="MIAIGQFVFYIPFFIMISILFYYIKWTKKKFSVLLASLPAVYFT
                     YQIFSFRHWETTSVLITHIIELTLSVIFLIIWIYFLYKNQN"
     gene            2112..2375
                     /locus_tag="ACG842_002478"
     CDS             2112..2375
                     /locus_tag="ACG842_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0248978.1"
                     /translation="MDHVVLEIDLHLINNIRVIYYVVSNSVEQRVLTNKINGILAKKD
                     VHRFNNGEGSYYSIPVEKIIYTTVKVREDLDTKKAYEPIFTTY"
     gene            2399..2587
                     /locus_tag="ACG842_002479"
     CDS             2399..2587
                     /locus_tag="ACG842_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0248979.1"
                     /translation="MNPYEQLIQDLIDGKIEKIDVKREELMAFREAWLKLENRKYVVG
                     KASLNGNVTYHYDPTRLF"
     gene            complement(2670..2855)
                     /locus_tag="ACG842_002480"
     CDS             complement(2670..2855)
                     /locus_tag="ACG842_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0248980.1"
                     /translation="MNQEELFQKVKEMIKNGNFDGAKRFIEEHKEQLGPYKEKAQNLL
                     KDVNIDSVKNKFKNLFK"
     gene            3070..3441
                     /locus_tag="ACG842_002481"
     CDS             3070..3441
                     /locus_tag="ACG842_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HFQ0248981.1"
                     /translation="MKVIKYLIGLAAISGILLFGLKYYTEGSYGEIPGILDQLNPLVE
                     KGEVYVKTQKPEEVNEYGTARYVQKAADANGKERTVEFNGLSVLKENHYLKISNKGAH
                     IETYEEVPREKVPEKALAIIG"
     gene            complement(3591..3663)
                     /locus_tag="ACG842_002482"
     tRNA            complement(3591..3663)
                     /locus_tag="ACG842_002482"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3628..3630),aa:Gln,seq:ctg)
     gene            complement(3709..4137)
                     /locus_tag="ACG842_002483"
     CDS             complement(3709..4137)
                     /locus_tag="ACG842_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0248982.1"
                     /translation="MEKFTHKKMDPNEIPIIFVRDCKGNVQGKVSINEWNERRRPATL
                     NELEIKLYRQSLVYYADQEYEKATDLLKFLIARTEYTRFEYIERLANIYHIMNEPVKE
                     YQLLDTVLSVAELIALPAGLEKKLVRRLLRVKQQLSDQEK"
     gene            complement(4197..4955)
                     /locus_tag="ACG842_002484"
     CDS             complement(4197..4955)
                     /locus_tag="ACG842_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HFQ0248983.1"
                     /translation="MKKKTFKKLLFLGGTAALLLSGTSYYLHIHSPEHTMNTAQKSST
                     QKTKKTKEKAEVLLDVPLESQFDSPSLENGCEVTSLSMLLSFYGYETTKNQLAEQLDY
                     VPVFNADGTHGDPNEGFVGDISGGDWAMVVYVPPVASLAQQIVQTDYHTFPKTDAQLD
                     DIKKALSKGNPVWTSVTINFEVPDETDFMTWTTNNGEVRVTPLVHACVVTGYDKENIY
                     VNDPYGVKNRAVPINDFSAIFTAMGGQMLTLEKS"
     gene            5250..5447
                     /locus_tag="ACG842_002485"
     CDS             5250..5447
                     /locus_tag="ACG842_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0248984.1"
                     /translation="MTEEMINLGEQYACKPIGFTKTVIGEVVSKMTNCAVVKVAQCAA
                     EDQELLDEKASMVVAKYDTFE"
     gene            5553..6035
                     /locus_tag="ACG842_002486"
     CDS             5553..6035
                     /locus_tag="ACG842_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0248985.1"
                     /translation="MVTFFTVLLFLFVVVMFLFLWLVRKEIIYRTVRNRWVYLVIPFL
                     VSLVIWYTLISHPTADELAKGILSAMIFISFLLDSRGITEEGLVLNSFDKKGVPFSEI
                     NKIVLYQPKGSKIVKMNFFRNGWRGPMLKFSASLEELVPFLSQRLNDEAEIDIMIDPE
                     "
     gene            complement(6093..6689)
                     /locus_tag="ACG842_002487"
     CDS             complement(6093..6689)
                     /locus_tag="ACG842_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HFQ0248986.1"
                     /translation="MTTFTDTLKNRRSIYHLGRNVSLSNEELTTLIKEAIKESPTAFN
                     AQSTRAVILFGDAHEKLWEITEEALRPLTPAEAFPNTQNKLAGFKNGYGTVLFFKDTD
                     VVKGLQEQFELYADNFPDWSEQSNGIATANTWVALVDKGLGANLQHYNPVIDEAVAKE
                     WNIPSNWKLRSQLVFGSPETPAEEKEYMNDADRFRVFG"
BASE COUNT         2346 a         1061 c         1357 g         2157 t
ORIGIN      
        1 gaatttgatt ctgcaatggc attgatcaag gagttatcac caacgtattt ttgagaaatc
       61 gttgatagag tatcacctga ttgaacggta taagcttcgt ccgcatgagc agtcgttccc
      121 atgaagaatg cagcgccggc agccaaagtt gttccaaaaa gtaaagtttt aagtgatgtc
      181 atgaaaaatc tcctttattc gttaaaaagt tttatttgtt gtgttcaacg aggaatactt
      241 taacacgtga aaatatttca taggtaaaag aagcatgaca atttatgaca aatccacgtt
      301 ttttgtaaca gaatgatgtt cgctcgatgt gaaaaatgat gaggaatttc acataatgca
      361 gaaataatgg gattagaaca ggtgttctga tgagaaaaga aatgtataaa taacttgaaa
      421 gaaattgctt ttgttcatca ttttatttac atattgcgta tattttttgt atttttgctt
      481 gagatgttac aataaaagaa aaaggaggcg gttccaatga gtaaaagata ccagaacatc
      541 atggtagcag ttgacggatc aagacaatcg atcaaagcgt tcgctgaagc ccttgactta
      601 gcaaaggaca atgaagccca cttatttatc gtgtcaatca tcaacaaagt tgagctgacc
      661 catagtgcct atgccttttc aaaaatctat gcagatgaaa aacaaagaac agaagtagcc
      721 atgctgaaaa aaatcaatga tgccaaagag tacggcattg ctgagatcca cgcaatagtt
      781 gaaactggag atccaagaaa tttgattgcc aatgtcatcc ctggacaaga aaatattgat
      841 ttgatcgtta tgggagcgac aggaaaaggt gctatacaac aagcattagt tggatcgacc
      901 gcttcttatg tcgtatcaca tgccccttgc agtgtgttgg tcgtgaagta atgagataag
      961 ggaggaatag aaaatggttc gtcctcaaga agtccgtgcg ccaaaagaaa aaatagaaat
     1021 tttagcaatt attgaagacg gcacacaaac aaagaaggga tactcgatcg ctttagtaaa
     1081 gtggaaagga aaaaaaggtg ttgctctgag atgggatgga gataaccaac aagataaagg
     1141 attccctgtc acagctaacg gttatcatcc agcgtggttt gttctgcctg ataaatttat
     1201 ggaattatac agctatgatt atgcaagagc cgcagcatca atcaaagcat tagataaatt
     1261 aacagaagag cagaatgaaa tggaggaaaa ataaaaaaga cgaaccaatc agcctttaga
     1321 aaactgatta gttcgaggag taaaaatgaa aaaaggttat ttgggatgaa actagaataa
     1381 tgcgtataac ttaaataaaa cttaaagcaa taaaatggca agagggtgag gcaaatggaa
     1441 gcaggaaaag tattacagga agtaaaagca ctatgtgcag ataaaaaata tgaagaagcg
     1501 aaaagcctga tcgaaaagaa caaagaaaat ttggaagaaa agtacccgat cgcacaagaa
     1561 tttatagaat taaaacaagg aacgatcctt caccgattca aacatttttt tggtataaca
     1621 gaatagaaaa aacgattaaa gatcggcaga aatttgctgc atctttaatc gttttctttt
     1681 taaacatgcg agggattcaa ttctgattct tatataaaaa atagatccaa ataataagaa
     1741 aaattactga aagcgtcaat tcgatgatgt gtgttattaa tacagacgtt gtttcccaat
     1801 gcctaaaaga aaaaatttga taagtaaaat atacagcagg taaagaggcc agtaaaactg
     1861 aaaacttctt ttttgtccat tttatgtaat agaaaagaat gcttatcata ataaaaaaag
     1921 gaatataaaa aacaaattga ccgatcgcga tcatactatt gcctccgata aataaagtcc
     1981 atgatacaaa ataacacttt gtgcgaaatg gcgcaagcag ttttgtggat ttatccggta
     2041 aaatctgtat cgattttgaa attagaggag aaacttgcta taatcaaagt gaaagaagga
     2101 gtgggatttt catggatcat gtagtgttag aaatcgattt gcatttgatc aataatattc
     2161 gggtgattta ctatgttgta agtaacagtg tcgaacaacg tgtactgacg aataaaataa
     2221 atggtatttt agcaaagaaa gacgttcacc gattcaataa tggtgaagga agttattata
     2281 gtatcccagt agaaaaaatt atttatacga cagtcaaagt cagagaagat ctagatacca
     2341 aaaaagcata tgaacctatt tttacaactt attgattgac agcagatgga ggaaaaaaat
     2401 gaatccatat gaacaactta ttcaagattt gatcgatgga aaaatcgaaa agattgatgt
     2461 aaaaagagaa gaattaatgg cttttcgaga agcgtggctg aagttggaga atcgtaaata
     2521 tgtggtggga aaagcctcac tgaacggcaa tgtgacttat cattacgatc cgacacgttt
     2581 attttagtat gaagtattca gaaaagaacc aaaggaggac gatgcagcag cattgtcctc
     2641 ctttgatttt acaaaagtga aagttttact tatttgaata gatttttaaa tttgtttttg
     2701 acactgtcga tatttacatc ttttaaaaga ttttgggctt tctctttata aggtcctaat
     2761 tgttctttat gttcttcgat aaatcgtttt gcgccatcga aattgccgtt cttaatcatt
     2821 tccttgactt tttgaaataa ttcttcttga ttcattttga acacatcctt tgctatcaac
     2881 atgatttttt acatggacaa aacgagaaaa actgatttat ccacacgtca ataatatcaa
     2941 ataacgatca tttttactaa aaaaacactc gattctcgct tctaaagtag gagaaaaatc
     3001 tccaacttgg gacggatgga atttctttaa ctgacccata tactgaatat ataacaagga
     3061 ggtagaaaga tgaaagtgat taaatatttg attggactgg cggcgattag tggtattttg
     3121 ctatttggac tgaaatatta tactgaagga agttatggag aaatccctgg tatacttgat
     3181 cagctaaatc cattagttga aaaaggagag gtctatgtaa agacacaaaa accagaggaa
     3241 gtaaatgaat atggtacagc aaggtatgtg cagaaagctg ctgatgccaa tgggaaagaa
     3301 cgaacggttg aatttaatgg tctttctgtg ttaaaagaaa atcattattt gaaaattagc
     3361 aataaaggag cacacataga aacgtatgag gaagttccta gagaaaaagt tccggagaaa
     3421 gcattagcaa ttattgggta aatcagtttt tgtattaaga atacgcccga ttttattatt
     3481 cataatttaa ttatgtaaac taaatacaag tagtaaaaat aatacaataa tccccctgca
     3541 aaaagattgt tcttttacag ggggttcatt ttttcagcaa gcacatcaaa ttgggatggc
     3601 agggttcgaa cctacaattg cttgattcag agtcaagtgc cttaccagtt tggctacatc
     3661 ccatttattc aacaaaataa tcatagcact ttcctaaaaa aacgcaagtt atttttcttg
     3721 atcggatagt tgttgcttta ctcgtaaaag gcggcgtact agtttctttt ctaatccagc
     3781 aggaagggca atcagttctg ctactgacaa gacggtgtcc aataactgat attctttgac
     3841 aggttcattc ataatatgat agatgtttgc taaacgttca atatattcaa agcgagtata
     3901 ctctgttcga gcaattagaa atttcaataa atcggtggct ttctcgtact cttgatctgc
     3961 gtaataaaca agagattgac gatacaattt gatttccagt tcattgaggg tagcaggtct
     4021 gcgacgctcg ttccactcat taatagagac tttaccttga acatttccct tgcagtcacg
     4081 gacaaaaata atcggaattt catttggatc cattttttta tgtgtgaatt tttccattat
     4141 ctacgctccc ttcaaaggtt tggtatcttg agtataaggg atttttgttt gaattactag
     4201 cttttctcca aagttagcat ctgtccaccc attgcagtga aaatcgccga aaaatcgttg
     4261 atcggtactg ctcgattttt tacaccatag ggatcgttga cataaatatt ttctttgtca
     4321 tatccagtga ctacacaagc atgaacaaga ggagtgactc ttacttctcc attatttgtg
     4381 gtccaagtca taaaatcagt ttcatctggg acttcaaaat tgatagtcac agaagtccag
     4441 acaggatttc ctttagatag tgcttttttg atatcatcga gttgagcatc ggtttttgga
     4501 aaagtgtgat aatccgtttg gacgatttgc tgagcaaggg aggcaactgg gggaacatag
     4561 acgaccatag cccagtctcc gccactgata tcaccaacaa atccttcatt aggatcgcca
     4621 tgagtcccat ctgcattgaa aacagggacg taatccaatt gctcagctaa ctggttttta
     4681 gttgtttcat aaccataaaa agataaaagc atagaaagcg aagtgacttc acagccattt
     4741 tctaaagaag gagaatcaaa ctggctctct aatggaacat ctagtaaaac ttctgctttc
     4801 tcttttgtct ttttcgtttt ttgtgttgaa cttttttgtg cagtgttcat tgtatgttct
     4861 ggagaatgga tatgtaggta ataacttgta ccgcttaata aaagagcagc tgtgccccct
     4921 agaaacaata gttttttgaa cgtttttttc ttcatttgaa atctcccttt tcagctgttt
     4981 atctttccta ttataaatgt atttttattg gaaatggaag tattatattt taatatgtag
     5041 gaaataatta gtttttaagg aatatgtttt aattttttga aaaaaattct tctaaataca
     5101 acagctgttt taattgtgaa aacgaaaaca gtacgctata atgcctgtaa ggttgatttt
     5161 taactgtaaa taaaaatcaa tgagttaaat ctccaaagaa atagcagtgg taatgtggag
     5221 aaaagttcca gcagtttagg aggattttga tgacggaaga aatgattaat ttaggagaac
     5281 aatatgcatg caaaccaatt ggttttacaa aaacggtcat tggtgaagtt gtatcaaaaa
     5341 tgacaaactg cgccgtggtc aaagttgccc aatgtgctgc agaggaccaa gaattattag
     5401 atgaaaaagc aagcatggtt gttgcaaaat atgatacatt tgaataaaga aaacaattgg
     5461 acgtgcggcg tgctgcaagt ccaatttctt tgagaaggta aatcagtatt cttttcctat
     5521 tttctggaga agaaaaagcg aggcgagttg tcatggtaac attctttacg gttctattgt
     5581 ttttattcgt tgttgtgatg tttttatttc tttggctggt tcgaaaagaa attatttatc
     5641 ggactgtgcg aaatcgatgg gtctacttgg tgataccctt cttagtgtca ctagtcattt
     5701 ggtatacact catcagtcat cctacagcag atgaattggc aaaagggatc ttgtcagcaa
     5761 tgatttttat tagttttttg ttggacagta gaggaatcac agaagaagga cttgttttga
     5821 atagctttga caaaaaaggt gttccttttt ctgagatcaa taaaattgta ttatatcagc
     5881 caaaaggttc taaaatcgta aaaatgaatt ttttccgaaa cggatggcga ggaccgatgc
     5941 tgaaattttc tgcatcatta gaagaattag ttccattttt gtcccaacgc ttaaacgatg
     6001 aggcagagat tgatattatg attgatccag aataaaaaca gaggttgcga caagatgttt
     6061 gtcacaacct ctgtttttta tttcaaataa aattatccaa atacgcggaa gcgatcggca
     6121 tcgttcatgt attctttttc ctcagccggt gtttctggtg aaccgaatac taattgcgaa
     6181 cgtagtttcc agtttgaagg gatattccat tcttttgcaa ctgcttcgtc aataactggg
     6241 ttgtaatgtt gcaagtttgc gcccaatcct ttatcaacta atgctaccca agtattagct
     6301 gttgcgattc cgtttgattg ttctgaccaa tctgggaagt tgtctgcata taattcaaat
     6361 tgttcttgta gaccttttac cacgtcagta tctttgaaga ataatactgt accataacca
     6421 tttttgaaac cagctaattt gttttgtgta tttgggaaag cttctgctgg agtcaatggg
     6481 cgaagtgctt cttcagtgat ttcccaaagt ttttcatgtg catctccgaa aaggataact
     6541 gcgcgtgttg attgagcatt gaaagcagtt gggctttctt taattgcttc tttgatcaaa
     6601 gttgttaatt cttcattaga aagtgaaaca ttacgtccta gatgatagat tgaacggcgg
     6661 tttttcaaag tgtcagtaaa agttgtcata ataaaatttc tcctttgatt tgcgttttgc
     6721 atttttttaa ttgattacga aatgaagtat agcaccttac aaaaagtaag taaacagata
     6781 tgctcacaaa aaataagcga agttcaaaaa acaattttaa taagaaaaat cgttatgtaa
     6841 ggattttctt agttatcgct ttcattgttg tgttttagga atttttgctt atttgaaaaa
     6901 aacagctgat atataattat t
//
LOCUS       DAZKLM010000116         6831 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3764
            SAMEA115706913-rid22582563.denovo.121, whole genome shotgun
            sequence.
ACCESSION   DAZKLM010000116 DAZKLM010000000
VERSION     DAZKLM010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706913
            Sequence Read Archive: ERR13196647
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6831)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6831)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287823.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287823.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,004
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,849
            CDSs (with protein)               :: 2,849
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 41 of 96
            Pseudo Genes (incomplete)         :: 52 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 15 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6831
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706913-rid22582563.denovo.121"
                     /isolate="VRE3764"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            528..722
                     /locus_tag="ACG842_002488"
     CDS             528..722
                     /locus_tag="ACG842_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0248987.1"
                     /translation="MKTTLVLFYKKHPYFTLLINILLASVIGISVEYLINKDFIGSCF
                     YTALFLGLLEAFSIYKKSKK"
     gene            913..1162
                     /locus_tag="ACG842_002489"
                     /pseudo
     CDS             913..1162
                     /locus_tag="ACG842_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001206386.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1406..2236)
                     /locus_tag="ACG842_002490"
     CDS             complement(1406..2236)
                     /locus_tag="ACG842_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HFQ0248988.1"
                     /translation="MFRHQKELQFEAKPDRPNPLIAKYLQELIGGQYGEMSVAMQYLF
                     QGWSCRGEEKYKDMLMDIATEEIGHVEMLATMVARLLEGAPVEDQEAAMQKDPTIGAI
                     ISGMNPQHAIVAGLGPRPADSVGNPWSGAYIIASGNLLADFRANLNAESQGRLQVARI
                     YEMIDDRGVKDMLSVLLARDSYHQNLWECAVKELEEKEGSILVPSTFPRDKERKDIAY
                     DFYNFSEGDQSSEGSWASGKALDGEGEYQWLKEPKIEGAAPKLEPVTPKMYGTPPFKK
                     "
     gene            2444..3778
                     /locus_tag="ACG842_002491"
     CDS             2444..3778
                     /locus_tag="ACG842_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFQ0248989.1"
                     /translation="MKKLLFTGLLASSALFLLGGCGSSNASDASSSAANSSEKTSDGR
                     KNIDFWSFWGSGARKEAIEEIIEDFNQSQNEIHVNYKYQPWGDIWTKSLSAITAGNAP
                     DVIVQDINSVAQRAEAQQATNLSKYVEKDTSKDFYPQLWDTVEYEGDPYAIPFNTDTQ
                     VIFYNKKIFKEAGISEQDLPTTWDELEETARKLDKKEGKEFTRIGFYPLWNLGADVWA
                     LNADNGTSWFDKDEKVKIDTKNKVEALEWIQDWQDYYGKNTINKLEAEFGSGVSDPFI
                     SGLVAMRAQNINYYSSLMENAPDDFDFGVMQLPEKEEGSGHWSWGGGFVLEVPYGAKD
                     PEASYEFIKYLSSPEVQEKFGEKSFDIMASKTANENLVNNDNLTDKGKMIYQMADENF
                     KNTVITPVPLDAPDYTTLVNEQIDQILLGTKSPKEGLADAQKAVENLVKQNQ"
     gene            3819..4730
                     /locus_tag="ACG842_002492"
     CDS             3819..4730
                     /locus_tag="ACG842_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0248990.1"
                     /translation="MKRLSIRKKKEARWAYLFISPFIIGFLVFMLGPMLFSVIGSFTD
                     YNLTSKMNFIGLANFKRMFVHDDLFWKSLYNTVYFVIFNVPLTTIFSVFLATVLNQKI
                     KGISVFRTAFYLPAVLSGVAVYILWMQLLSPSAGLINTFLGWFHIPGPAWLFDPQWTK
                     PALILMKIWSSGGAMLLYLATLQNVPRSLYESAEIDGAGVWGKFKSITLPLITPIIFY
                     DVITSTIGSFQIFQEAYVMTKNGTGGPANSLLFYNLHMWNKAFVAFDMGYAMAMSMIL
                     FLIVLVLTFVNLKLAPKWVYYSGGDGK"
     gene            4792..5580
                     /locus_tag="ACG842_002493"
     CDS             4792..5580
                     /locus_tag="ACG842_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014834452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0248991.1"
                     /translation="MTVIGIILIIPLLWMVFTSLKPMEEIVRYPPTFFPEKIVWENYL
                     DTIAAFPFWRYARNTLFITVLVVIGNVLSNSFIAYGFAKLDFPGKKLMFALVLSTMMI
                     PGFVTMIPQYVLFSKIGWVGTYLPLIVPSFFGNAFNIFLMRQFYLSINNELIEAAEID
                     GANHLYIWSHLMLPLTKPALITIAINSFNAAWNDFLGPLLYIQDQEKYTLQIGLQVFQ
                     NQATTQWNYLMAGATLVLIPTILLFFFAQRYFIEGMDLTGGSKG"
     gene            5577..6193
                     /locus_tag="ACG842_002494"
                     /pseudo
     CDS             5577..6193
                     /locus_tag="ACG842_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339120.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
     gene            6195..6713
                     /locus_tag="ACG842_002495"
     CDS             6195..6713
                     /locus_tag="ACG842_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HFQ0248992.1"
                     /translation="MLRLIAKIDDSIINKFAKQRKTDSRKINFLKFISNISNGGFIWL
                     LLAAVLVLIPDTREYGLAILIGSLIYFFVGLLFIKKTTRRKRPYEKNRRLVLYTSKPK
                     TSSFPSGHTALAFLSVAALFELSPVAGILAFLFACLIAYSRIFLLLHYPTDILGGAIL
                     GSVIGILCAAVI"
BASE COUNT         2051 a         1185 c         1371 g         2224 t
ORIGIN      
        1 gttattttat aataaagata tgatgactaa tttagctcaa ctattccagc gtcagctgaa
       61 gatattgcaa ttcccgattc cttaatcatt tatctggatg agacagaaag tgaagttgca
      121 acagatgtgc atgtagttga ggataaaaat gatagtttaa aaattaatag ttcagatact
      181 ttactaagag taagtcctat cttttcagct gttatttaga tgcaagtagc aactaagtta
      241 ggtagtactc ctgcaatgga taggtatttg tgtgttcgat atataagtgg tcgaagtatc
      301 aaggttatgt atactggatg ggagatagaa gagttaaaaa ttggaggtca ccgccacaaa
      361 tggaatttag atttagaggt accttagatt tggtatagtt attaaacaca agatggcctg
      421 gtttgtaggg aaagtcgaag agtatttttc cacttctgcg cccctttaat ttttctagta
      481 atgccgtgaa tttgtgatta attggaaata tgaagaaagg agagtctatg aaaacaacac
      541 tagttctatt ctataaaaag catccttatt ttacacttct gataaatata ttgttggcta
      601 gtgttatcgg gatttctgtt gaatatctaa taaataaaga tttcataggt tcttgttttt
      661 atacggcttt atttcttgga cttcttgaag cattttccat ttataaaaaa tcaaaaaaat
      721 aaaatagatt gccgtaagag atctttttca tttcattttt ttctgaaaac ccgttgtttc
      781 tgtcagtgaa gcagcgggtt tttagattta ttgtctttag acctaattga gcgcgccgag
      841 cgattgaaat acaaaaccac ttgctatgcg ggtggagcaa taaagggaaa tagtttagcc
      901 cgtacaaagt agatgtgtaa ataccacatt gtattcacac caaagtatag aagaaaaata
      961 atttataatc aatatcgagc caatattcag gagatcatca agagattgtg caaatctaaa
     1021 atataaattt gggtatcgac acttttgggt ggaaggatac tatgtgacaa aagtaggaca
     1081 aaatgaagcg accatcaaaa agtacattca agaacaagaa aaacatgata gtgcgttaga
     1141 taagctgagt gtacgagagt aagacgatcc ttttaagggc taaggagtag tacataaacc
     1201 ttttaagagg tgggcaaaac tggcaaaggc aatatggctt gaacataaaa tatgaaagcc
     1261 agcgtcttta ggcgctggcc ggtgatttga gcttatagct ctagagaaaa ccaccctttt
     1321 gaagggtggt tatgattaat gactttagtc agttttgctc acaaagcgtg tggaacaaaa
     1381 aacgggtgtt tctacgggta gatatttatt tcttgaatgg aggtgtccca tacatttttg
     1441 gtgttactgg ttctaatttt ggtgcagcgc cttcgatctt aggttctttc aaccattgat
     1501 attcgccttc gccatccagt gctttgccac tagcccaaga accttcacta ctttggtctc
     1561 cctctgagaa gttatagaag tcataagcaa tgtcttttcg ttctttgtca cgcgggaaag
     1621 tacttgggac taagattgaa ccttcttttt cttctaattc tttcactgca cattcccata
     1681 gattttgatg atagctatct cttgcaagga gaacagacag catatctttt acgccacgat
     1741 catcgatcat ttcgtaaata cgtgctactt gaagtcttcc ttgtgattcg gcatttaaat
     1801 tagctctaaa gtctgctaac aagttgccac tcgcaatgat ataggcaccg ctccatggat
     1861 tgcccacgct gtcagcagga cgaggtccta agccagctac gatcgcgtgc tgtggattca
     1921 ttccagagat aatggcaccg attgttggat ctttttgcat cgctgcttct tgatcttcaa
     1981 caggcgcacc ctctaataaa cgagcaacca ttgtcgccaa catttccacg tggccgattt
     2041 cttctgttgc aatgtccatc agcatatctt tatatttttc ttctcctcgg cagctccagc
     2101 cttggaacag gtactgcata gctacactca tctcgccata ttggccgccg atcagctcct
     2161 gcagatattt cgcgattaat ggattcggac gatctggttt tgcttcaaac tgcaattctt
     2221 tctggtgtct aaacatcaaa attcctccta atctattttt aatacattct cattctgcta
     2281 cgaaagatta aaaaattcaa agaaagcgct cgcgaattat ataaagtatt ggtatcatta
     2341 ataatttttt tagaatcata ccttataaat tgttattttc agaacaaaat gatatcgtta
     2401 tattaaaacc ggcatcggtt acattaaaat ttggaggtaa aatatgaaaa aattattgtt
     2461 tacagggttg ctggcaagtt cagcattgtt tttattagga ggatgcggat caagtaatgc
     2521 aagcgatgcc agttcaagtg cagcaaattc ttctgaaaaa acaagtgatg gaagaaaaaa
     2581 tattgatttc tggtcctttt ggggatccgg tgcacgaaaa gaagctatcg aagaaatcat
     2641 tgaggatttc aatcaatcac aaaacgagat ccatgtaaat tataagtatc agccatgggg
     2701 tgacatctgg actaaatctt tatctgcaat aacagctgga aatgcacccg atgttattgt
     2761 acaggacata aattccgttg cccaaagagc agaagcacag caagcaacta atctctccaa
     2821 atatgtagaa aaagacactt ctaaggattt ttatccgcag ctttgggata cagtagaata
     2881 tgaaggtgat ccttatgcga tcccattcaa tacagataca caagtcattt tttataataa
     2941 aaagatattt aaagaagctg gaatcagtga acaagatcta ccgacaactt gggatgaatt
     3001 agaagaaaca gcacgtaaac tagataaaaa agaaggaaaa gaatttacac gcatcggttt
     3061 ttatccatta tggaatttag gggctgatgt atgggcgcta aacgcagata acgggactag
     3121 ttggtttgac aaagatgaaa aagtcaagat tgatactaaa aataaagtgg aagccttgga
     3181 gtggatccaa gactggcaag attattatgg gaaaaatacg atcaataaat tagaagccga
     3241 atttgggtct ggtgtatctg atccatttat ttccggatta gtagccatgc gtgcccaaaa
     3301 tatcaattat tactctagtt tgatggaaaa tgcaccggat gattttgatt ttggcgtgat
     3361 gcagctccca gaaaaagaag aaggttcagg ccattggtcg tggggtggcg gattcgtctt
     3421 ggaagtacca tacggagcaa aagatcctga ggcctcttat gaattcatca aatatctttc
     3481 ttctccagaa gtacaagaaa aattcggcga aaaaagtttt gatatcatgg cgagcaagac
     3541 agccaatgag aatttagtga ataatgacaa cctaacggat aaaggaaaaa tgatctatca
     3601 aatggcggac gaaaacttta aaaatacggt gatcacacca gttcctttag atgctccgga
     3661 ttacacgaca ttagtgaatg aacagattga ccagattctt ttaggaacaa aatcaccaaa
     3721 agaaggattg gcagatgcac aaaaagcagt agaaaactta gtcaagcaaa atcaataatc
     3781 catttttgac tgtctaggta cagaggaggc aagaaaattt gaagcgttta tctatccgaa
     3841 agaaaaaaga agcaagatgg gcctatttat ttatctcgcc ttttattatc gggttcttgg
     3901 tatttatgct agggcctatg cttttttcag ttattggtag tttcaccgat tataatttga
     3961 catccaagat gaatttcatt ggtctagcaa atttcaaaag aatgttcgtt cacgatgatt
     4021 tattttggaa atcgttatac aacaccgttt atttcgtcat cttcaatgta ccgctgacga
     4081 cgattttcag tgtgttttta gccacagtac tcaatcaaaa aatcaaagga atcagtgtat
     4141 tccgtacagc tttctattta ccggcagtct tatcaggagt agcagtctac atcttatgga
     4201 tgcagctgct ttctccatct gcaggattga tcaacacatt tctgggctgg ttccatatcc
     4261 ctggaccagc ttggctgttt gatccacaat ggacgaaacc tgcgttgatt ttgatgaaaa
     4321 tatggagttc tggaggagca atgctacttt acttagctac attacaaaat gttcctagat
     4381 ctttatatga atcagcagaa atcgatggag caggcgtttg gggaaagttc aaaagtatta
     4441 ctttgccatt aatcactcct attatttttt acgatgtgat cacctctacg atcggttctt
     4501 tccaaatttt tcaagaagct tatgtcatga cgaaaaatgg aacaggcgga cctgcaaatt
     4561 ctttgctttt ttataatctg catatgtgga acaaggcgtt cgtagctttt gatatgggtt
     4621 atgcgatggc tatgtcaatg atcctgtttc tgatcgtgtt ggttctgacg tttgtcaatt
     4681 tgaagcttgc acctaagtgg gtctattatt ctggaggtga cggcaaatga tggattattt
     4741 caaaaagacc agtgtgaatc ggcaaacaaa aaaatactaa gttatgtcct catgacagta
     4801 ataggaatca ttttgatcat tccattgctt tggatggtgt ttacttcatt gaaaccaatg
     4861 gaagaaatcg ttcgttatcc tccgaccttt ttcccagaaa aaatcgtatg ggaaaactat
     4921 ctggatacga ttgccgcctt tcctttttgg cgctatgcaa gaaatacatt attcatcact
     4981 gtgctagtag tgatcggtaa tgtcctttca aattctttta ttgcttatgg atttgcgaag
     5041 cttgattttc ctgggaaaaa gctgatgttt gctttggttc tttctacgat gatgatcccg
     5101 gggtttgtaa cgatgatccc tcaatatgta ttattctcca agatcggctg ggtaggtact
     5161 tatttgcccc tgatcgttcc atcgttcttc ggcaatgcgt tcaatatatt tctgatgcgt
     5221 caattttact tatcgatcaa taatgaattg atcgaagctg ctgaaatcga cggagcgaat
     5281 catttgtata tttggagtca tttgatgctt ccattgacga aacctgcctt gattaccatt
     5341 gcgatcaatt ccttcaacgc agcttggaat gatttcttag gtcctctgtt atatatccaa
     5401 gatcaagaaa aatacacgtt acagattggc ttgcaagttt ttcagaacca agcaacgaca
     5461 caatggaatt atctgatggc aggagcaaca cttgtcttga tcccgacgat tttgctgttc
     5521 ttttttgcac aacgttattt cattgaaggg atggatttga caggaggttc aaaaggatga
     5581 tgaatcgatc gattggtaaa aaagccaatg attatttctt agaaatcatt gaatggattc
     5641 cacgtcttat ccatttacag ctactatggt tcctgtgcgt cttgcctgtt ttcacattag
     5701 gaactgcaag tcgaacagtt ctgtatatga tttatcatta caaaaaaatg aagagaaaaa
     5761 gctcggttca ctattctgga aaaagtttcg tgagaacttc tctctttatg gaaagcaaga
     5821 tagtttagca agtgtttatt ttcttcttct attgatcgat tatcagattt tccgctattg
     5881 gggtggggga attggctata cattgatgta tactacgttg tttttgcttc tattaagcgc
     5941 gcttctcttt agttacaaaa tcttgcttca actggaacaa aaagaagctt cttggattac
     6001 tgcatttttc ttgtttttta atgatttcaa aagcgcgctt ttctatttgg caggcacgtt
     6061 gatccttctt gtcgcattct ggtttgcagg accaatctat tttgctttat taggtttcag
     6121 tttcttgttt tattttcaag tcctgctttt tatccatcgt acgcaagaaa aggttcttaa
     6181 aaaggaggaa taacatgttg cgtttgattg ctaaaatcga tgatagtatt atcaacaagt
     6241 tcgccaagca acggaaaacg gatagtcgaa aaatcaattt tttgaaattt atcagtaata
     6301 tctctaatgg tggttttatt tggttgctcc ttgctgctgt tttagtcttg atacctgata
     6361 ctagagaata tggtctagcc attcttatcg gcagtttgat ctattttttt gttggtttgc
     6421 tttttatcaa aaaaacgaca agacggaaaa gaccttatga aaaaaatcgt cggcttgtcc
     6481 tttacacaag taaaccgaag acttcttctt ttccttcagg acatacagcg ctagcctttt
     6541 tatcagtagc tgcgttgttc gaattaagtc cagttgcagg aatattagcc tttttatttg
     6601 cttgtttgat cgcttattcg aggatttttt tacttttaca ttatcctact gacattttag
     6661 gaggagctat tttaggaagt gttattggca tcctatgcgc tgccgttatc tgaattaatt
     6721 gtacgaataa agcatgagtg cgtaaatgcg catttgtgct ttattttttt gtttcgagga
     6781 aagcgtagtt gtttattcat ttgtcttaat aacttaagtt ttgttaaatg a
//
LOCUS       DAZKLM010000117         6644 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3764
            SAMEA115706913-rid22582563.denovo.122, whole genome shotgun
            sequence.
ACCESSION   DAZKLM010000117 DAZKLM010000000
VERSION     DAZKLM010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706913
            Sequence Read Archive: ERR13196647
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6644)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6644)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287823.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287823.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,004
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,849
            CDSs (with protein)               :: 2,849
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 41 of 96
            Pseudo Genes (incomplete)         :: 52 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 15 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6644
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706913-rid22582563.denovo.122"
                     /isolate="VRE3764"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(1..859)
                     /locus_tag="ACG842_002496"
                     /pseudo
     CDS             complement(1..859)
                     /locus_tag="ACG842_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEfa10 family transposase"
     gene            1102..1557
                     /gene="tnpA"
                     /locus_tag="ACG842_002497"
     CDS             1102..1557
                     /gene="tnpA"
                     /locus_tag="ACG842_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HFQ0248993.1"
                     /translation="MSNDDKSLAYTRWNCKYHIIFTPKYQRKVIYGQLRKDLGKILRK
                     LCEMKDVEIIEAHAMLDHIHMLVRVPPKICISSFMGYLKGRSAVLIHEQHANLKYKYG
                     NKSFWSKRYYVSTVGLNQKTNEKYIREQEADDRIRYIISKREYVEPFKK"
     gene            1822..1920
                     /locus_tag="ACG842_002498"
                     /pseudo
     CDS             1822..1920
                     /locus_tag="ACG842_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558064.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            complement(2143..2373)
                     /locus_tag="ACG842_002499"
     CDS             complement(2143..2373)
                     /locus_tag="ACG842_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="resolvase"
                     /protein_id="HFQ0248994.1"
                     /translation="MDEAMDHPLLNKFIKDLILQIFSMIDEQEPTESKRHRAQGIIKI
                     AKRMFSIKGVLSYTVLIQKIPNHLVYKNNVED"
     gene            complement(2603..3421)
                     /locus_tag="ACG842_002500"
     CDS             complement(2603..3421)
                     /locus_tag="ACG842_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFQ0248995.1"
                     /translation="MSYIYLIKENYPFLTKSEKKVADFILNSGKTIIYSTMSDIKKNT
                     KVGDATIIRFCQKIGFSGFSDLKIEIAKEDFSQKKEHPTSVKYYDEIANSLTEALYST
                     IRLLDEEKLNEAIKMITQSKNIYIFGVGSSGNTSLDLENMFLRVGVQAKAVLDPHFQA
                     QVASLLTVNDLVIIFSLSGKTKDTYDSLKIAKKNGAKIIAITNYIHSPIGKSADLVLQ
                     TAIEEFLNGGSLAGKISQLYICDLLVHGYEQNNNIDSVELREKVLRSIIDKSIE"
     gene            3582..4271
                     /locus_tag="ACG842_002501"
     CDS             3582..4271
                     /locus_tag="ACG842_002501"
                     /EC_number="5.1.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009385 - N-acylmannosamine-6-phosphate
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006051 - N-acetylmannosamine metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019262 - N-acetylneuraminate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HFQ0248996.1"
                     /translation="MDKKRFLDLISKGLIVSCQALPGEPFYKEEESLMPYFALAAQRA
                     GAVGIRANSVRDIIEIRKCVSLPMIGIIKRDYPPESPFITATMREVDELVETGVEVIA
                     LDCTLRNRHDGLSINSFITKIKEKYPHQLFMADISTFEEGKNAYKAGIDFVGTTLSGY
                     TEESKKQDGPDIDLIKKLVEEGICVIAEGRIHTPEQAKEIKKIGVAGIVVGGAITRPQ
                     EIASRFVKALS"
     gene            4285..5787
                     /locus_tag="ACG842_002502"
     CDS             4285..5787
                     /locus_tag="ACG842_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFQ0248997.1"
                     /translation="MFKKFSQLGRAFMLPIAILPVAGLLLGLGGALTNESAINAYPFL
                     DQPWLHTILSIMSYAGNAVFANLALIFAIGIAVGLANGDKGTAGLAGGVAYLVYTATI
                     SGFLELFSAKDATLDTGVVGSIVVGGTVAFLHNRYRKIELPQFLGFFGGSRFIPIVSS
                     LAAIIIGSFFYLIWPPIQNGLVVAGEQIAQMGSLGTFLYGFLLRLTGAVGLHHTIYPL
                     FWYTSLGGTETVAGSTIAGAQNIFFAQLADPNHTGLFTYGTRFFAGRFATMMFGLPAA
                     CYAMYRAIPKKNRKKNGGLYFSGALTSFLTGITEPVEYMFLFVAPWLYVIHAFLDGLS
                     FYFADILNIRVGNSFSGGLIDFLLFGVLQGNDKTNWLKLIPFGIAWAFIYFFVFTFFI
                     KKFKVAIPGMEEESEVHSEKVINKNTSLHEESLIIIEALGGKENIESVEACATRLRVA
                     VTNGEIIQKQTIKDTGATAVFEVKGGIQAVFGGKADLLSQEINQILGKDN"
     gene            5800..6276
                     /locus_tag="ACG842_002503"
     CDS             5800..6276
                     /locus_tag="ACG842_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HFQ0248998.1"
                     /translation="MFEIFKKKKQETFYAPCSGKIISITEVNDPVFSQKLLGDGFAVI
                     PDGSKIYAPLAGRVTNIFPTKHAINIKSNSGIDYLIHIGIDTVELQGEPFSICVQEND
                     EVTPETSLVDVDFDKIIKSGKDTSVIVVFPEKNQFNDLIIDNGRIAHGNSCGKITF"
     gene            complement(6373..6483)
                     /locus_tag="ACG842_002504"
                     /pseudo
     CDS             complement(6373..6483)
                     /locus_tag="ACG842_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         2174 a         1032 c         1203 g         2235 t
ORIGIN      
        1 acttgtcctc gataagaatt gtagcgccgt gatttacgtg tgtatgtcgt tacttttatt
       61 cctagcttct tgatcaggcg ctgaactttc tttttattga tagagaaacc gcggtttctt
      121 agttcattgg tcatccgtaa acagccataa tccttgtgct tctcccgaac tttaagcatt
      181 tccgcttcaa tctcttgatc tggattttta cggtccaatc gtttttgcca gtacataaag
      241 attgccttag ggaactttaa agtttctaga agctcaacta atttaaacgg tcctcggagg
      301 cttgcgatga ttcgtgcgat tgcttcattc gtcgttgttg ggcttcctgt tttcgcagct
      361 tcctcaattc ttttaaaaag gcattttcaa tttgaaggga acggactcgt ttctccaact
      421 cctcgatgcg ttcacgttct ttagacgttg ctttagttgt agattttttc ttatttttgg
      481 gttcattttt cttagacaag ataggtggac atcctttcgt ttttgagagt ccatcgatcc
      541 caccctcacg gaattgacgc atccaactag cgatcaaatt agggttgttc atgttaagtg
      601 tgttggcaac ttcacgatag gacagctcac ttgttagata taactctatc gcatccagct
      661 taaattgaac agaatatttt ttcttttgtc gctttcttac tagaccctct tcaccaaatt
      721 cttgataggc attgatccag cgcttcactt gtgatggagc cttaatggaa tatttctttg
      781 ccagataatc acttccgcct ttaccatcca agtaatcatg aacgatcttt aatttaagtt
      841 caaaagtata cttagccata aaaaataccg acctccaaaa gttagatttt ttggtctaac
      901 ttttgggggt cggtacaaaa cttaaaagtt ttagaccctt ttattttctt aatgacttca
      961 gtcagttttg tgcgtaaagc gtgcgaaaca agaaaaccgc gcgtttctac gcgtggaaga
     1021 agtatcttta gcgataaaaa ctcctttcga tatactagtg agtggctacc aaccgctcat
     1081 cagatcgaaa ggagtttttt catgtcaaat gacgataaaa gtttagcata tactcgttgg
     1141 aattgtaagt atcatattat ttttacacca aaatatcaac gaaaagtaat ctatgggcaa
     1201 ttacgaaaag atcttggaaa aatacttagg aagttgtgcg aaatgaaaga tgtggaaatc
     1261 atagaagcac atgcaatgct agatcatatt cacatgcttg ttcgagtacc accaaaaata
     1321 tgtatatcca gttttatggg gtatctgaaa ggtcgtagtg cggtactaat ccacgagcag
     1381 cacgcaaatc tcaaatataa atacggaaac aaatcctttt ggtcgaagag atactatgtg
     1441 agtacagtcg gattgaatca aaagacaaat gaaaaataca tccgtgaaca agaagctgat
     1501 gatcgcattc gatatattat aagtaaaaga gagtacgtag aaccatttaa aaaataaaga
     1561 gaagtaagtg agtgaagatg agcagttggc ggtcttttaa aaatcccctt ttaaggggca
     1621 gcgagtgtcg accccttcta gggggattaa aaatacaccc gtcctcacgg gtgtatgggt
     1681 tactgataga cttgatttta actggtgtca gaaacttatg tccagaaagg gtgatgaaat
     1741 tttggcaatt tacggctatt caagaattag cacaagcaac caagattaca aaacacaaat
     1801 tcaaacatta gaaaatgctg gcgcagaaat aatcttccta gaaaatcact ggattatcga
     1861 acacctttgg aagtattttt gagttacgta agtattgatg atctgtctaa cttaatttga
     1921 caattaagag tatatttact tcagatggta aggcaacaaa ataaaataca ataggcagat
     1981 gaactaataa ataaaatctg tattaaatac agataaatta atataaaaat caaaaggatc
     2041 ataaactttt aacaagtgat gaaatgggtg gaaattgatt cttatagaca gtttgtctca
     2101 taacattgaa gtattttgca attttagcat tgaaacactt ttttaatctt caacgttgtt
     2161 cttataaaca agatgattgg gaatcttttg aattaacact gtatagctta gaacaccctt
     2221 tatagaaaac attcgcttag caatcttaat aattccttgt gcccgatgtc gtttagattc
     2281 agtaggttcc tgctcatcga tcatggaaaa aatttgaagg atcagatcct taataaattt
     2341 atttaagaga ggatgatcca ttgcttcatc catactcgat aaactagtga taatgagcta
     2401 cacgtttttc tttcaagttg atcaatagct tccacgataa aaatatcttt agataaaatc
     2461 aagaaccgta ggaactacag ggatatttgg tcaataagag ccaccgctat tagtaaaaaa
     2521 taagctatca agtatgctct catcccaaga attgcctact tcaatcgtta aaaaccgtta
     2581 ggtttagcta agtgggggta gttcactcaa tacttttatc aataatagag cgcaaaactt
     2641 tttctcttaa ttcaactgaa tcaatattat tgttttgttc ataaccatga actaatagat
     2701 cacaaatata aagttgtgat atttttccag ctaaagatcc accattaaga aattcttcaa
     2761 tggctgtttg taaaactaag tctgcagact ttcctatagg tgaatgaata tagttagtta
     2821 tagcaataat ttttgcgcca ttttttttag ctattttcaa tgaatcataa gtatctttcg
     2881 ttttcccaga taaagaaaaa atgattacta aatcattaac tgttaataaa gaagcgactt
     2941 gggcttgaaa atgagggtct agtacagctt ttgcttgaac tcctacgcga agaaacatat
     3001 tttctaaatc aagactagta tttccactag atcccactcc aaatatatat atgttttttg
     3061 attgagtgat cattttaatg gcttcattca atttttcttc gtctaaaagc ctaatagtgg
     3121 aatagagagc ttctgttaaa ctattagcaa tttcatcata gtactttaca ctagtcggat
     3181 gttctttttt ttgactaaag tcttcctttg caatttcaat ttttaaatca gaaaaaccag
     3241 aaaaaccaat tttttgacaa aagcgaataa tagtagcatc gccaactttt gtgtttttct
     3301 taatatcact catcgtacta taaataatag ttttcccaga atttaaaata aaatcagcga
     3361 cttttttttc agacttcgtt aaaaatggat aattttcttt aattaaatag atataactca
     3421 tacaaactcc tctacaattt caacttttct ccattatact ccaaaataat aaaaaaaact
     3481 cctaaatact ccatttttat ctttactttt atgaaagcgg atgctatatt gtttttggag
     3541 ttattatcca aaaataataa aaaattggag cgtttaggat aatggataaa aagaggtttt
     3601 tagatcttat ttcaaaaggt ttgattgttt catgtcaagc attacctggt gaaccatttt
     3661 acaaagagga agaaagttta atgccttatt ttgctttggc tgctcaaagg gctggagcag
     3721 ttggtatacg tgcaaattct gttcgagata ttattgaaat tagaaagtgt gtatcattac
     3781 caatgattgg aattattaaa agagattatc caccagaatc tccattcatt actgccacta
     3841 tgagagaagt agatgaatta gtagaaaccg gtgttgaggt aatcgctctt gattgcactc
     3901 ttagaaatcg acatgatgga ctatctataa attcttttat tactaaaata aaagaaaaat
     3961 atcctcatca gttatttatg gcagatattt ctacatttga agaaggcaaa aatgcatata
     4021 aagcaggaat agattttgta ggaactactt taagtggata tactgaggaa agtaaaaaac
     4081 aagatgggcc agatatagat ttgattaaaa aattggtaga agaagggatt tgtgtaattg
     4141 ctgagggaag gattcatact ccagaacaag caaaagagat aaaaaaaata ggagttgcag
     4201 gtattgttgt gggtggtgct attactcgac cacaggaaat tgcatcaaga tttgttaaag
     4261 cattaagtta gggggagaaa taaaatgttt aaaaaatttt cacaattagg acgcgctttc
     4321 atgttaccaa ttgctatatt accagtagcc ggattactat tgggactggg aggagcgtta
     4381 acaaatgaga gtgctataaa tgcttatcca tttttagatc aaccgtggtt gcatacaatt
     4441 ttaagtatta tgagttatgc tggaaatgca gtttttgcaa acctagcact aatttttgca
     4501 attggtattg ctgtaggact tgcaaatgga gataaaggaa cagctggttt agctggtgga
     4561 gtagcttatc tagtatatac tgcaactatt agtgggtttc ttgaactttt ttcagcaaaa
     4621 gatgcaacac tagatacagg tgtagtggga tcaattgttg ttggtggtac tgttgcattt
     4681 ttacacaatc gttatagaaa aattgaatta ccacaattct taggtttttt tggaggatca
     4741 agatttattc cgattgtttc tagtttagct gcaatcatta ttggttcttt cttttattta
     4801 atttggccac caattcaaaa tggtttagta gttgctggtg aacaaattgc ccaaatgggt
     4861 agtttaggaa cttttttata tggtttttta ttgcgtttaa caggtgctgt tggactacac
     4921 cacaccattt accctttgtt ctggtataca tctttaggtg gtaccgaaac tgtagctgga
     4981 tcaacaattg ctggagccca aaatattttc tttgcccaac tagctgatcc aaaccacact
     5041 ggtctgttta cttatggtac tagatttttt gcaggaagat ttgcaacaat gatgtttgga
     5101 ttaccagcag catgttatgc aatgtatcgt gcaattccta agaaaaatcg taaaaagaat
     5161 ggtgggcttt attttagcgg ggcccttaca tcatttttaa ctggaattac tgaaccagtt
     5221 gaatatatgt ttttatttgt tgctccttgg ctttatgtga ttcatgcatt cttagatgga
     5281 ttatcttttt attttgctga tatcttaaat attcgtgtag ggaattcctt ttcaggtggt
     5341 ttgatcgact ttttattatt cggtgtactt caagggaatg acaaaactaa ttggttaaaa
     5401 ttaattcctt ttggtattgc atgggcattt atatatttct ttgtttttac attctttatc
     5461 aaaaaattta aggtagctat tcctggaatg gaagaagaat cagaagttca ttctgaaaaa
     5521 gtaataaaca aaaatacatc attacatgaa gagtctctta tcattattga agctcttggt
     5581 ggaaaagaaa atattgagtc tgttgaagct tgtgcaactc gtttgcgtgt tgctgttacg
     5641 aatggggaga ttattcaaaa acaaacaata aaagacactg gtgcaactgc tgtcttcgaa
     5701 gtgaagggtg gcatccaagc tgtttttgga gggaaagctg acttattaag tcaagaaatc
     5761 aatcaaatat taggaaaaga taattaggag gtttttaata tgtttgaaat tttcaaaaag
     5821 aaaaaacaag aaacttttta tgcaccttgt tccggaaaaa tcatttccat cacagaagta
     5881 aatgatcctg tcttttcaca aaaattacta ggtgatggtt ttgccgtcat acctgatggt
     5941 tcaaaaattt atgctccatt ggctggtcga gtaacaaata tatttcctac taaacatgct
     6001 attaatataa aaagcaactc aggcattgat tatttgattc atattggaat tgatacagtt
     6061 gaattacaag gagagccttt ttcgatttgt gttcaagaaa atgatgaggt tactcctgaa
     6121 acctcgttgg ttgatgtgga ttttgacaaa ataataaaat ctggtaaaga tacttctgtt
     6181 atagttgttt ttccggagaa aaatcagttc aatgatttaa taatagataa tggtcgaatt
     6241 gctcatggga attcatgtgg aaaaatcact ttttaaaaaa gtgtatataa aaagctataa
     6301 aataagctaa ataggatatt tgtcaaatga agttggtgaa cagaaagaac tgttttcttt
     6361 ctgttcattt ttttatttta ttagtaatga ttccccatgc caaatcatga tccgtaattt
     6421 aaagttataa aagcttcgaa atccattggc atttcgttgg aataccttga tatgattatt
     6481 caaatataaa atatagagag aagagagaga gggaagatat ttataaaggg caaagttatg
     6541 tttactcaac ttaggaaata gaaatagatc aatttatatt ctcttgtcaa tcgaatatga
     6601 accttagcac tatataccaa atatagtaaa atgctgaaag atgt
//
LOCUS       DAZKLM010000118         6489 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3764
            SAMEA115706913-rid22582563.denovo.123, whole genome shotgun
            sequence.
ACCESSION   DAZKLM010000118 DAZKLM010000000
VERSION     DAZKLM010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706913
            Sequence Read Archive: ERR13196647
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6489)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6489)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287823.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287823.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,004
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,849
            CDSs (with protein)               :: 2,849
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 41 of 96
            Pseudo Genes (incomplete)         :: 52 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 15 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6489
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706913-rid22582563.denovo.123"
                     /isolate="VRE3764"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            362..952
                     /locus_tag="ACG842_002505"
     CDS             362..952
                     /locus_tag="ACG842_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-gamma-glutamate biosynthesis protein"
                     /protein_id="HFQ0248999.1"
                     /translation="MEISSKEYLKAVLRMEKNLYIESKGQHTKLLKIMKSHPDYNAWR
                     YTRRMRITSYYYSKRKKNIIYAIMYIVSCRRMNSLGRKCGIECGENVFDEGVRFYHTQ
                     GIVINGNAIIGKNCYLYGNNCIGNDGIDPKCPVIGNNVRVCVGAKIIGNVKIANNVVV
                     AAGAIVIKDCLENGAILAGVPAKIIGYVNLTKVFLE"
     gene            979..2289
                     /locus_tag="ACG842_002506"
     CDS             979..2289
                     /locus_tag="ACG842_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HFQ0249000.1"
                     /translation="MRISIKRWSGADWTLFTLAFFAISIVLVYTFNAPRAILYLGDII
                     NIFVLLNALSFRRRKGIYASKNEMLIVLFLLVSICSSVINLENIGLIIWGIRNNTRMF
                     IVYINCATYLTIKDYYKVFAIIEKLFWISLPLCLIERFMVTYPTGTIVGDMIGGVFWN
                     YSGCNMPLNLILCVTVTVVTIGYFKGEKNFIHFILCVCAALFMSALAELKVFIPELFI
                     IFVAIVFTLKVDFKVVIKILSCVILVSIVLSFFITIFITLNDNGQSYADNFTIQGLLE
                     YASRDSGYDGVGDLNRITGISRVVDEIFNNNFRLVLMGVGIGNAEFTNFFVSEFYKSF
                     GNLHYQYFHHIWLFIEVGILGVSIFLFFIIKTFFNAKRSFMKDKDASLVKVIAIMSVF
                     FFVYNTTLRGETTGPLIYMILAMPEVVKRKEHYYSEKNTKGEIL"
     gene            2286..3005
                     /locus_tag="ACG842_002507"
     CDS             2286..3005
                     /locus_tag="ACG842_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 32 protein"
                     /protein_id="HFQ0249001.1"
                     /translation="MIPKKIHYCWFGGNPLPKKDRKCIESWKKYCPDYEIIEWNESNY
                     DLNKNRYMADAYKERKWGFVPDYARFDIIYNHGGFYLDTDVELIKSLDILRENHAYMG
                     FEGEIWVNGGIGFGAEEGHSVIKGLRDIYDSLSFYQNDGTLNLKPSPYYITDYLESIG
                     LVRDNSEQIINGLHIYPTDYFAAKDYDTGKIYKTSNTISIHQYNASWKTPKQKFVYRL
                     KSTIGQKKFNRLLALKKFLHL"
     gene            3020..4105
                     /locus_tag="ACG842_002508"
     CDS             3020..4105
                     /locus_tag="ACG842_002508"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFQ0249002.1"
                     /translation="MKKICFISGVISRSGGTERVGSIIANSLSDLGYDVTILSFWNSG
                     EPYFGLDKTIKVDYMLNPKTEGKLYRTYLYPVWKLHKYICKNEFDIVIDIDTLLSLYT
                     TRAIKGTKCKLISWEHFNYWTMEMLKEKKRFKAKKLIKKYAKRLVVLTEEDRQKHIDE
                     YSLSEDFVITMPNPCISNIEYEYHYDKKRFLAVGRLVEEKNFADMISAWKIVEKKVDD
                     WDLIIVGKGELEKDLRQQIKDLNLQRVTIAGHSDHVEEYYKSASCYVLSSVYEGFPMV
                     ILEAQSYGLPVISYDCKTGPRDLVHHNFNGMLVEDKNIDQLAKSMIMFTKNTDLAMRM
                     SLNAYNNVQKFNLKEITKQWVALIENV"
     gene            4241..5773
                     /locus_tag="ACG842_002509"
     CDS             4241..5773
                     /locus_tag="ACG842_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HFQ0249003.1"
                     /translation="MIINRKQNAKRNIITGTIFKLYQIIIPFFMRTLMIYQMGMEYAG
                     LNNLFTSIFQMLNLAELGIGAALTVSMYKPIAEDNRKEICALLNAYKKYFSIIGWIVF
                     TLGLLCIPFLRFLVKGSIPGNLNMTVLYLMYLINTVLSYWLFSYRKSLIYAHQRNDVI
                     NVSMLITFTFQFVMQAYVLIFLKNYYLYLLASILGQIVLNLMCAVVSQKMYPDYTPVG
                     ELEKETIDDIIKKVKGLVTNKVGGTILRSSDSIIISSFLGLSVLAIYQNYYFIISAIM
                     SIFAIIYESCIAGIGNSLIVESQEKNYIDFCDITTIIGWFLAISCACFITLFQPFMII
                     WVGSDNLMSISFVIFMIVHFFLYEIDQLIGLYKDAAGIWYADRFRPLITAMINIVLNI
                     ILVNWFGLYGVLAATIVSVLLVDLPWLLYNVFQNIFKFESAGKYVWKLISLCVIAFVA
                     SYLSYFACSYVNIGNFKVISLVINLVISVVISILVILLFNIRNKKFKYILKVLTSVLV
                     KR"
     gene            5951..6172
                     /locus_tag="ACG842_002510"
     CDS             5951..6172
                     /locus_tag="ACG842_002510"
                     /inference="COORDINATES: protein motif:HMM:NF012360.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DapH/DapD/GlmU-related protein"
                     /protein_id="HFQ0249004.1"
                     /translation="MGSSERIKIGNNVFIGMKSRILKGVHIGNNVVIGANSLVNKDVS
                     DDCVVAGNLAKVIMSLDEYYKKINNMKNP"
BASE COUNT         2270 a          695 c         1257 g         2267 t
ORIGIN      
        1 cattagcata tttaagcgga atgggtagaa ataggagatt ttaatcataa aaaactgtac
       61 tatagtgaat ttttggagat ttatattcca gttatatatt aatgattgtg atacttgtac
      121 tattgtaaaa attcgttagg tttaaataac tggaaatatc ggaaaatggg cattatacat
      181 cacggacatc ccttcttttg gaaaaaatgt tacgattttt ataagtaata ttgtatccgg
      241 tggaatcaac gatgaagata acatatccat cgttgtagat tcagtagcat tgaatgatgc
      301 tcttgttgca agaaatccga ataaaattgt taagtataaa ttgaattaat aggagatatg
      361 aatggaaatt agcagtaaag aatatttaaa agcggttctt cgtatggaaa agaatctata
      421 tatagaatct aaaggacaac acacaaaatt actaaaaata atgaaatcac atccggacta
      481 caacgcttgg cggtatactc gtcgaatgag aattacatct tattattatt caaagagaaa
      541 gaaaaatatc atttatgcga ttatgtatat tgtgtcttgc agaagaatga atagtttggg
      601 aagaaaatgt ggtatagagt gtggtgaaaa tgtatttgat gaaggtgtaa gattttacca
      661 tacacaagga attgttatta atggtaacgc cataatagga aaaaattgtt acttatatgg
      721 gaataactgt attggaaatg atggaatcga tcctaagtgt cctgtaattg gaaataatgt
      781 acgtgtatgc gtgggtgcaa agattattgg taatgtaaaa atagctaaca atgtagttgt
      841 tgcggcagga gctattgtta tcaaagattg tctagagaat ggtgcaattc ttgcaggtgt
      901 accggcaaaa ataatagggt atgttaattt aacaaaagta tttttagaat aatttttagc
      961 gaaagaaggt gaataacgat gcgtatttca ataaaaagat ggtcaggagc agattggaca
     1021 ttgttcacgt tggcattttt tgcaatctcc atagttcttg tatatacttt taacgcccca
     1081 agagcaattt tatatttggg agatataatt aatatctttg tgttattaaa tgcacttagc
     1141 tttagacgca gaaaaggaat atatgctagc aaaaatgaga tgctgatagt tttgttttta
     1201 ctagtttcta tttgtagctc agttattaat ttagaaaata taggattgat tatctggggg
     1261 atacgaaata acactcgtat gttcatagta tatataaatt gtgcaacata tttaacaatt
     1321 aaagattact ataaagtatt cgctataatt gaaaaattat tctggatttc actgccactt
     1381 tgtttaattg aaagattcat ggtcacctat ccaactggaa caattgtagg agatatgata
     1441 ggtggagttt tttggaatta ttcaggttgt aatatgccat tgaatttaat tttgtgtgtg
     1501 acagttactg ttgttacaat cggatatttc aagggagaaa aaaatttcat ccattttatt
     1561 ttatgtgttt gtgcagcact ttttatgtct gcattagctg aattgaaagt ttttattcct
     1621 gaattattta taatatttgt tgcaatagta ttcactttaa aagttgattt taaagtagtg
     1681 atcaagatat tatcatgcgt aatacttgtg tcgatagtat tatccttttt tataacaata
     1741 tttattacat tgaacgataa tggacaaagc tatgctgata atttcacaat tcaaggtctt
     1801 ttagaatatg cgtcaagaga ttctggatat gatggtgttg gtgatttaaa tcgaataact
     1861 ggtatctcta gagtagtaga tgaaatattt aataataatt ttagattagt cctaatggga
     1921 gtaggcattg gcaatgcaga gtttaccaat ttttttgtca gtgagtttta taaatcgttt
     1981 ggtaatcttc attatcagta ttttcatcat atttggttat ttatagaagt tggaatatta
     2041 ggagtttcga tattcctatt ttttataata aagacatttt tcaatgcaaa aagatctttt
     2101 atgaaggata aagatgcaag tcttgttaaa gttattgcaa ttatgtcggt attctttttt
     2161 gtgtataaca caacacttag gggtgaaaca acggggccac tgatttatat gattttagcc
     2221 atgcctgaag ttgtgaaaag gaaagagcat tattatagtg aaaaaaatac aaaaggagag
     2281 atattatgat tcctaagaag atacattact gttggttcgg gggaaatcca cttccgaaaa
     2341 aagatagaaa gtgcatagaa agctggaaaa agtattgccc tgattatgag attattgaat
     2401 ggaatgaatc aaattatgat ttaaataaga accgttatat ggccgatgca tataaagaac
     2461 gtaaatgggg ttttgttcca gattatgcaa gatttgatat tatttataat catggtggat
     2521 tttatttaga tacagatgtt gagctaatta agtcgttaga tatacttcgt gaaaatcatg
     2581 cctatatggg atttgaaggg gaaatctggg ttaatggcgg tattggtttt ggtgcagaag
     2641 aaggacattc tgttattaaa ggtttaaggg atatttacga ttcattaagt ttttatcaaa
     2701 atgatggtac attgaacttg aaaccgagtc cgtattatat tacagattat cttgaatcta
     2761 ttggattggt tcgcgataac agcgaacaaa taataaatgg cctgcatatc tatcctacgg
     2821 attattttgc tgccaaggat tatgatactg gaaaaattta taaaactagt aatacaataa
     2881 gcattcatca atataatgca tcttggaaaa cacctaaaca aaaatttgtt tatagattaa
     2941 aatcaacaat tggacaaaaa aaatttaaca ggttacttgc actgaaaaaa tttttacact
     3001 tataaaggag aaaagtactt tgaagaaaat ttgtttcatt agtggtgtaa tttctcgttc
     3061 tgggggaact gaaagagtag ggagtattat tgccaatagc ttgtctgatt taggttatga
     3121 tgtcactatt ttaagctttt ggaatagtgg agagccttat tttgggttag ataagacgat
     3181 taaggttgat tatatgttaa atcctaaaac ggagggaaaa ttatatagaa cttatttata
     3241 tccggtttgg aaattgcaca agtatatatg caaaaatgaa tttgatatag taattgatat
     3301 tgatactttg ttgtctttgt atactacaag ggcaattaaa ggaacaaagt gcaaattgat
     3361 ttcttgggaa cattttaatt actggacaat ggaaatgctg aaagaaaaga agagattcaa
     3421 ggctaaaaaa ttgataaaaa aatatgcaaa gaggcttgtg gttctcacag aagaagatcg
     3481 tcaaaaacat attgatgaat atagtttatc agaagatttt gttataacta tgccaaatcc
     3541 gtgtatatct aatatcgagt acgaatatca ttatgataaa aagagatttc ttgcagtagg
     3601 gcgattagtg gaagagaaaa attttgctga catgataagt gcttggaaaa ttgtagaaaa
     3661 aaaagtagat gattgggatc ttatcattgt aggaaaaggt gaattagaaa aagatcttcg
     3721 ccagcagata aaagatttaa atctgcaaag agtaactata gcagggcatt cagaccatgt
     3781 ggaggaatat tataaatctg catcttgtta tgtgcttagt tctgtttatg aaggatttcc
     3841 tatggttata ttagaagcac agtcttatgg attaccagtt atttcttatg attgtaaaac
     3901 tggtccgaga gatttggtac atcataattt taatgggatg ttggttgaag ataagaatat
     3961 agaccaatta gctaagagca tgataatgtt tactaagaat actgatttag caatgagaat
     4021 gtcactgaac gcatataata atgtacaaaa atttaatttg aaagaaatta caaaacaatg
     4081 ggttgctttg attgaaaatg tatagttttt tgtggttgtc caaacatgaa aattagcgta
     4141 attaatcaaa gagctagaag aaacctatta catttcaaga ttttagaaat tatatagtat
     4201 taggtaacaa taaaaaatag aaagtttgga gccatatgat atgataatta atcgtaaaca
     4261 aaatgctaaa aggaatataa ttactggaac aatttttaag ttatatcaga ttatcattcc
     4321 cttttttatg cgtacactta tgatttatca gatgggaatg gagtatgctg gattgaacaa
     4381 cctgttcaca tctatatttc aaatgttaaa tttagcggaa ttgggaatag gtgctgcact
     4441 aactgtaagt atgtataaac ctatagcaga agacaataga aaagagattt gtgctttact
     4501 gaacgcatat aaaaagtact tttcaattat tggatggata gtgtttacat taggattgtt
     4561 atgcattcct tttttgagat ttttagttaa aggttcgatt cctggaaatt taaatatgac
     4621 agttttgtat ttaatgtatt tgataaatac tgttttgtcg tattggttat tttcatatag
     4681 aaagagtcta atatatgcac atcaaagaaa tgatgtaatt aacgtgtcta tgctaattac
     4741 ttttacattc caatttgtaa tgcaggcata tgttctgatt ttcttaaaaa attactattt
     4801 gtatttattg gcttctattt tgggacaaat agttcttaac ttaatgtgtg ctgttgtttc
     4861 tcaaaagatg tatcctgatt atactcctgt tggtgagtta gagaaagaaa ctattgacga
     4921 tattataaaa aaagtaaaag gattagttac aaacaaggta ggcggtacaa tattacgatc
     4981 ttcagattca attattattt catctttctt aggtctttca gtacttgcga tttatcagaa
     5041 ttattacttt attatttctg ccataatgag tatatttgca ataatttatg aatcatgtat
     5101 agcaggtata ggtaacagtc ttattgtaga aagtcaagaa aaaaattata tagatttttg
     5161 tgatattaca acaattatag gttggtttct ggcaattagt tgtgcatgct ttattacatt
     5221 atttcagccg ttcatgatta tctgggtggg aagtgataat cttatgagta tttcatttgt
     5281 tatatttatg atagttcatt ttttcttata tgaaattgat cagttaattg ggttatacaa
     5341 agatgctgct ggaatatggt acgcagacag gtttagacca ttaattacag caatgataaa
     5401 cattgtatta aatatcatcc tcgttaattg gttcggatta tatggagttt tggcggcaac
     5461 cattgtttca gtattgttgg ttgatttacc ctggttatta tataatgttt tccaaaatat
     5521 atttaaattt gaatcggcag gtaaatatgt ttggaagctc ataagtttgt gtgtaatagc
     5581 ttttgttgct tcttatttaa gttattttgc ttgttcgtat gttaatatcg gaaatttcaa
     5641 agtgataagt ttagtgataa atttagttat atcggttgtc atatcgattt tagttattct
     5701 tctgtttaat atccgtaata agaagtttaa gtatattttg aaagttctta cgtcggtatt
     5761 ggtaaaaaga tgagaagaga catcagaatg atatataaag taactacaat ttttaggctg
     5821 ccgaataggc gaacgatgta tagtttttgt tccaacgaag actcttgttg aaattggaga
     5881 tgatatgaag gagttacaat tttgactcat gtttatgatt gatcggtttt aaaagtagta
     5941 tggagaagct ttgggatcat cggagaggat caaaataggt aataatgttt ttataggaat
     6001 gaagtctaga atactaaagg gtgtacatat aggaaataat gtagttatag gtgcaaatag
     6061 tttagtaaac aaggacgtat cagacgattg tgttgttgca ggaaatctag caaaagtaat
     6121 tatgtcccta gatgaatatt ataaaaagat aaacaatatg aagaatccgt aaaacttgtg
     6181 aaactatacc gagaataata tgggaaaaat attgatgaaa aagcattata tgagttttta
     6241 gggctgttta cagatggaga tgacaatttg tcgaagagtt gggagcaatg caaaatttag
     6301 taggggatta taaacagact gacaaggtat ttaaagaaca tgcacaagta tttcataata
     6361 ttgaagaatt tttgaatact gtgagatagg agttgctaca ttgagaaaag tatgtatttt
     6421 tccaatgtaa aagattacga gtttgaaaac gacaccataa tattattttt taagaagata
     6481 ttatagagc
//
LOCUS       DAZKLM010000119         6364 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3764
            SAMEA115706913-rid22582563.denovo.124, whole genome shotgun
            sequence.
ACCESSION   DAZKLM010000119 DAZKLM010000000
VERSION     DAZKLM010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706913
            Sequence Read Archive: ERR13196647
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6364)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6364)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287823.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287823.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,004
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,849
            CDSs (with protein)               :: 2,849
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 41 of 96
            Pseudo Genes (incomplete)         :: 52 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 15 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6364
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706913-rid22582563.denovo.124"
                     /isolate="VRE3764"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(99..1229)
                     /locus_tag="ACG842_002511"
     CDS             complement(99..1229)
                     /locus_tag="ACG842_002511"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003871 -
                     5-methyltetrahydropteroyltriglutamate-homocysteine
                     S-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HFQ0249005.1"
                     /translation="MSKNRPFTVDHVGSFLRPQSIKEARKKVQEGLLSKEELRAIEDQ
                     EIKKLVEDQKAVGIKGITDGEFRRGFWHLDFLEELNGIEGYVPETGYNQTFHGKAAPA
                     YNIRVVDKLSFNPNHPFLKDFAFLKEVVETDDTFVAKATIPSPTMILRQEILSNDGSS
                     KIKEIYPDLSDFYEDLSSTYKAAIHAFYEKGCRYLQFDDTNWAFLADQTKREALLQKG
                     IDPKEMAHICTEIINNALEDKPEDLTITTHICRGNHASSWLFSGGYEPIAEELFSTNY
                     DGFFLEYDSDRAGDFTPLRHWSNKESKVVLGLVTSKFPDLEDTEKIKERIKEAQQFVP
                     LENLSISPQCGFASTEEGNHLTEEEQWKKIQLLQQVAKEVWL"
     misc_binding    complement(1289..1516)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(1878..3080)
                     /locus_tag="ACG842_002512"
     CDS             complement(1878..3080)
                     /locus_tag="ACG842_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFQ0249006.1"
                     /translation="MPKIKNIYQDKKTKKWFFRAYLGIDENGRKVQKTKRGYASQKDA
                     KIAYDKYMETHDFNKTIPNQLCSTNQMTFEEFYKDRFVKWYERQVKSQTYENAQFIFE
                     KRMQFFYHFRVRDITSHDIEDWLFELSQTESRNSRKKVNGETLSKSYINRIRGHLKIV
                     LDRAVKEGVIAKNPVEDVSSLPSENKKVEFWEHSEFQKVMNELKDDKIQTHHRKIVYE
                     MLFYTGIRIGELEALSWSNVDFEKNTITIEKTLIYNTKDDWYFSTPKTKCAYRTIGLG
                     KKLSLKLRQWYDLQKRIGNFEYVAQLDGTFTPPYSFANWLKEAAKKAGVKPIKLHALR
                     HSHVAFLIEQNIQPLSIQERLGHANIQITLGTYGHLYAKSDQQVVSAIDNVQEGAILS
                     KNDNLLPV"
     gene            complement(3140..3343)
                     /locus_tag="ACG842_002513"
     CDS             complement(3140..3343)
                     /locus_tag="ACG842_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HFQ0249007.1"
                     /translation="METKCDFMVNRAILIEMGFKPSQAARMIKESKAYLARIEGIDFY
                     NNRQVGVVPSRVIEHLFHIQVAE"
     gene            complement(3485..4549)
                     /locus_tag="ACG842_002514"
     CDS             complement(3485..4549)
                     /locus_tag="ACG842_002514"
                     /inference="COORDINATES: protein motif:HMM:NF014537.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HFQ0249008.1"
                     /translation="MDSLQEEVVPVNRVLIDYLTIHFFSNQHLKLIEEIIEMPIERFE
                     ELDYAPKGYIGQYVWNQVITIRYSIDDAVKGTVMEFSGQGCKHLARWLKTRKTNWQQF
                     FRKVLDYQGNFTRIDFTLDDFVGSLSIPELKHKVILGHVWTTFQVSESHGGTDIINNE
                     SNGETLYLGSKKSQCRFCFYQKDHEQRKRRGIPLEEAKVKNRFELRYRKEKAQSLAKI
                     ISKTHDLTKLFFELLNGAICFYDRDPNDLGAKVDAKWAAFISNYGAITISLDTIPQSF
                     EKTMSWLIHGVSPSLVFIAQVDQTFHSSLLPLIIEQGEMNPRQIKLLQNMKADPEYYQ
                     EEVEYYERKLQSISNEKTSY"
     gene            complement(4610..4717)
                     /locus_tag="ACG842_002515"
     CDS             complement(4610..4717)
                     /locus_tag="ACG842_002515"
                     /inference="COORDINATES: protein motif:HMM:NF013540.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0249009.1"
                     /translation="MNQVNQHDLGEAIRVSREERGWTQRYLAEKVGISR"
     gene            4880..5692
                     /locus_tag="ACG842_002516"
     CDS             4880..5692
                     /locus_tag="ACG842_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0249010.1"
                     /translation="MEIDKQAVGNRIRQIRQELKLSMEKFGKLIGDLPRSTVNNWERG
                     INLPKTETLHQIAEIGHVTNEYLLYGDQENQYILEMLQKKAGKLDPKIEGLIVDEIKH
                     ADLVSEKEMDRMIEFFITNLIPPTEQDQFTFQCIDEKKQLYLGSTNFGKEAQLYLQHD
                     NQNHILHMMPFTFSNFSVDRILVYLANQKSYSYFGKHLPKELGEKAILLYSINQLTSD
                     VRIAPLVYSEETASYQYTEDNQYLLEQQYLYLPFVMEVEKERLLNAAYPSSK"
     gene            complement(6066..6257)
                     /locus_tag="ACG842_002517"
     CDS             complement(6066..6257)
                     /locus_tag="ACG842_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0249011.1"
                     /translation="MKQKFFIAIIATKKFHRGKRFGQKWKFPPKVTAPQLKSIKELEF
                     ENALVICMTCTDEVFRKHF"
BASE COUNT         1901 a         1304 c         1004 g         2155 t
ORIGIN      
        1 aaataaataa tataaaaggt aaataaaaaa gagtggattt ctccactcca tgaaaagccg
       61 actttttggg acgctttttt ttgccaagcc tcacattttt atagccaaac ttcttttgcg
      121 acttgctgca ataattggat tttcttccat tgttcttctt ctgttaaatg gtttccttct
      181 tctgttgaag caaacccaca ttgaggactg atacttagat tttccaatgg tacaaattgt
      241 tgtgcttctt ttattcgttc ttttattttc tccgtatctt ccaaatctgg aaacttagaa
      301 gtaactaatc ctaaaacaac tttactttct ttgttactcc aatgtcttaa aggagtaaaa
      361 tctcctgctc ggtcagaatc gtattccaaa aagaaaccat cataatttgt tgaaaacaac
      421 tcttctgcaa tgggttcata tccaccggaa aacagccaag aagaagcgtg attcccgcgg
      481 cagatatgtg ttgtgatcgt caaatcttct ggtttgtctt ccagtgcgtt attgataatc
      541 tctgtacaaa tatgggccat ttctttcgga tcaattcctt tttgaagaag tgcttctctt
      601 ttagtttgat cagctagaaa cgcccaattt gtatcatcga attgcaggta acggcaacct
      661 ttttcataaa aagcatgaat cgctgctttg taagtagagg acaagtcctc ataaaaatca
      721 gatagatcag gataaatctc cttgattttt gaagaaccat cattagatag gatctcttgt
      781 cgtaaaatca tcgtgggact agggatcgtt gcttttgcaa caaaagtatc atctgtttcc
      841 actacttctt tcagaaaagc aaaatctttt aaaaatgggt gatttggatt gaaagataat
      901 ttatctacta cccgaatatt ataagctggt gctgcttttc cgtggaatgt ttgattatat
      961 ccagtttctg gaacatagcc ttcgatacca tttagttctt ctaaaaaatc aagatgccaa
     1021 aatccacgcc gaaattcacc atctgtaatt cctttgatgc cgactgcttt ttggtcttcc
     1081 actaattttt ttatttcctg gtcttcgatt gcacgaagtt cttcttttga aagtaagcct
     1141 tcttgaactt tttttcttgc ttctttgatc gattgcgggc gtaagaaact tccgacatga
     1201 tctactgtaa atggtctatt tttgctcatt tcactcactc ttttctttgt tttttgtaaa
     1261 aaaaaatcct tttactagca aatactagta aaaggacgat tatactcgtg gtaccacctt
     1321 ttttcagaaa aaacattctt tctctcagcc tgacaataaa atcagtggtc ttggtaacga
     1381 aagactgttc gtttttgcct acctttcgac aaaaaatcct ctaaggccat tttcgacagt
     1441 caacttctag ctttttacac caaccaaagc ctctcttgaa ccattttctg tctactttcc
     1501 ttctcatcgg atactattag aataaaatta gaatagcatg tttgaaagaa aagtcaatag
     1561 ggaataagaa caaacaaaaa ctaatttgtt attcttagta atttgtttgt ggaatacaat
     1621 aaagcttttc taatatgata attgttcagt cgtcctttca tcttagatca cttccttaaa
     1681 atgatcatac ccgtttcaac cacataaaaa aggacttaat tttgaaaaat ttcccatcaa
     1741 aactaagtcc tttttattat agttatgcaa atttgcttat tttctcgcta tttccgatac
     1801 atcttgaaca ggagcaattc gactgaaaac actggtgcaa aagcatttat ttgtcttatg
     1861 gtagaaccgt ggaaaactca gactggtaat aaattgtcat tttttgaaag aatagcgccc
     1921 tcttgaacat tatcaatcgc acttaccact tgttgatctg actttgcata taagtgaccg
     1981 taagttccta aagtgatttg aatattcgca tggcccaatc gttcttgaat agatagcggt
     2041 tgaatattct gctcaattag aaatgcaaca tgagaatgtc gtagcgcgtg aagcttaatc
     2101 ggtttgacac ccgctttttt agcagcttct tttaaccagt tcgcaaaaga ataaggtggc
     2161 gtaaatgttc cgtccaactg agcaacatat tcaaaatttc caatacgttt ttgcaagtca
     2221 taccactgtc tgagttttaa ggaaagcttc ttccccaacc caatcgttcg ataagcacat
     2281 ttagttttgg gagtcgaaaa gtaccaatca tcttttgtat tataaatcaa tgtcttctca
     2341 atcgttatcg tgtttttctc aaaatctaca ttcgaccaag acaaagcttc aagttctcca
     2401 atccgaatgc cagtataaaa gagcatttca tacacaatct tccgatggtg ggtttgaatc
     2461 ttatcatcct tcaattcatt catcactttt tgaaactcac tgtgttccca aaactcaact
     2521 ttcttattct cagaaggaag cgaagaaaca tcttcaacgg gatttttagc aatcaccccc
     2581 tctttaacag cccgatccaa tacaattttt aggtgccctc gaatccgatt gatataagat
     2641 tttgataacg tttcgccatt aacttttttc cgtgaattac gtgactctgt ctggcttagt
     2701 tcaaataacc aatcctcaat atcgtgactt gtaatatctc gaacacgaaa atgatagaaa
     2761 aactgcatac gcttttcgaa aatgaattgc gcattctcat atgtttgact tttgacctgt
     2821 ctctcgtacc attttacaaa cctatccttg taaaactctt cgaaagtcat ttgattggta
     2881 gaacacagtt gattaggaat tgtcttgtta aaatcatgtg tttccatgta cttgtcataa
     2941 gcaattttgg catctttttg tgaagcataa cctcgtttag tcttctgaac ctttcttcca
     3001 ttttcatcta tacctagata cgctcggaag aaccattttt ttgtcttttt atcttggtaa
     3061 atatttttaa tttttggcat cgttccattc ctccctgtac ttgagaaaag ctactttaat
     3121 agtaccgcgc ttgtactgtt tattccgcaa cttgaatgtg aaaaagatgt tcaattactc
     3181 gggatggcac cacaccaact tggcgattat tataaaaatc aataccttca atccttgcaa
     3241 gatatgcctt gctctcttta atcattcgtg cagcttgact aggtttaaag cccatctcta
     3301 tcaaaattgc acgatttacc atgaaatcac attttgtttc cattctctca cctcctgatt
     3361 ctttactcct tttaaaataa tcctctttat aatctattgt ttctcattcg atggttgtgt
     3421 tatactcgtc ttgtcgaggc ggagtacgtt gcacaacttg ttgtgtaagc agtactttgc
     3481 ttttttagta gcttgttttc tcatttgata tactttgtaa ttttcgttca taatactcaa
     3541 cctcctcctg ataatactcc gggtctgcct tcatattttg gagaagtttg atctgtcttg
     3601 gattcatttc tccttgttca ataatcaaag gcaacaagga agaatgaaac gtttgatcga
     3661 cctgtgcaat aaaaactaat gatggtgaaa caccatgaat taaccaactc atagttttct
     3721 caaaactttg aggaattgta tctaaagaaa tggtgattgc gccataattg ctaataaagg
     3781 ctgcccattt ggcatcaact tttgcaccta gatcatttgg atcacggtca taaaaacaaa
     3841 ttgctccatt aagtaactca aagaacagtt tggttaaatc atgggttttt gaaataatct
     3901 ttgccaagct ttgcgctttc tcttttcgat accgtagttc aaagcgattc tttacttttg
     3961 cctcttctaa aggaattcct cgtcgcttcc gttgttcatg gtccttctga taaaagcaaa
     4021 aacgacactg actcttcttt gagcctaaat acaaggtctc accatttgat tcattgttta
     4081 tgatatccgt accgccatga gattcactca cttgaaaagt ggtccacaca tggcctagta
     4141 ttaccttatg cttaagttct ggaatgctga gactccccac aaaatcatct aaagtaaaat
     4201 caatccgtgt aaagttcccc tgataatcta ataccttacg aaaaaactgt tgccaatttg
     4261 tttttcgtgt ttttagccat cttgctaagt gtttacagcc ttgacctgaa aactccatca
     4321 cagttccttt aaccgcatcg tcaatggaat accgaatcgt gattacttga ttccacacgt
     4381 attggccaat atagcccttt ggcgcataat ccagttcctc aaaacgttca atgggcattt
     4441 caataatctc ttcaattagc tttaaatgtt gattcgagaa aaagtgaatg gttagataat
     4501 caatcaaaac acggttaact ggaacgacct cttcttgcaa actatccaac acaagattca
     4561 atttttcttc acttaattgt ctcgttcctt tttctacttt cgataataac tatctcgaaa
     4621 tccccacttt ttctgctaaa tatcgttgtg tccaccccct ttcttctcga gaaactcgaa
     4681 tggcttcacc gagatcatgt tgattaactt ggttcatcga caaacctcct tctgctattt
     4741 acttgtcaaa gaacctattg aacttatttt aagtacattt ttattgtacg cctgtattta
     4801 ttttaagtca atatgttttg acttttttta agtacacacg tatactaaat ttaagaactt
     4861 atcctgagga ggagttttca tggagataga caagcaagca gtcggcaatc gaattcggca
     4921 gattcgccaa gaattaaagt tgagtatgga gaaatttggc aaactaattg gtgatttacc
     4981 gcgaagtaca gtcaataact gggaacgtgg gatcaacctt ccaaaaaccg aaacccttca
     5041 ccaaatcgca gagataggtc atgtcaccaa tgaatatctt ttgtatggtg atcaagagaa
     5101 tcaatacatt ttagaaatgc tccaaaaaaa ggcaggtaag cttgatccta agattgaagg
     5161 tctcatagta gacgaaatca aacacgctga ccttgttagt gaaaaagaaa tggatcgaat
     5221 gattgaattt tttattacaa atctcattcc accaactgaa caagaccagt ttactttcca
     5281 atgtatcgat gaaaaaaagc agctttatct tggttcaact aattttggta aggaagccca
     5341 gctctatttg caacatgata atcaaaatca tattctgcac atgatgccct ttaccttctc
     5401 caactttagt gtggatcgca tactcgtcta tttagcaaat caaaaatcct attcctattt
     5461 tgggaaacat ttacccaagg aactaggtga gaaagccatc cttctatact caattaatca
     5521 attgaccagc gatgtgcgaa ttgccccgtt ggtctattca gaagaaaccg cttcttatca
     5581 gtacacagag gacaatcaat atctactaga acagcaatat ctttatctac cattcgtgat
     5641 ggaagtcgag aaagaacgcc tgttaaatgc agcgtatcca tcatcaaaat gaggtgacaa
     5701 aattgataag cacgtgacaa agaaaatact gctaggacac tccccaatgg gtagttgtca
     5761 cagtttcctg ttctttgtta gccccctgtt agaaccgggg gctttattcc tgcagtccgg
     5821 ctacgccttc ctgcaataga caaaactcca gatcgggtaa ttcgcttgtg gcgaaacccg
     5881 acctggagtt tgtctctgta ttttgaaccc taaaaattaa tcaaacagac tgatattctc
     5941 ataatcagcg cgaaataagc cattaagtac agttttccta cctatttttg ataacccgct
     6001 ggttagttaa tattcctttt ccgagagaga taaatgattg gttaaatcta tatacatctc
     6061 aacctttaaa aatgttttcg aaatacttca tcagtacatg tcatacaaat taccagtgca
     6121 ttctcaaatt ctagttcttt aattgatttc agttgaggag cagttacttt tggcggaaat
     6181 ttccattttt gtccaaacct cttcccccga tgaaatttct ttgttgcaat tattgcaatg
     6241 aaaaattttt gtttcaaaaa tatacataat tacttcctcc aataaaaact atctacctct
     6301 caaattattt ttctctaata agaaaaacag aaaaggtaat aagatacaac agtgatgatt
     6361 ccta
//
LOCUS       DAZKLM010000120         5993 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3764
            SAMEA115706913-rid22582563.denovo.125, whole genome shotgun
            sequence.
ACCESSION   DAZKLM010000120 DAZKLM010000000
VERSION     DAZKLM010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706913
            Sequence Read Archive: ERR13196647
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5993)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5993)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287823.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287823.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,004
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,849
            CDSs (with protein)               :: 2,849
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 41 of 96
            Pseudo Genes (incomplete)         :: 52 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 15 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5993
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706913-rid22582563.denovo.125"
                     /isolate="VRE3764"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(580..1083)
                     /locus_tag="ACG842_002518"
     CDS             complement(580..1083)
                     /locus_tag="ACG842_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HFQ0249012.1"
                     /translation="MRYVSKFLLGIYLFFLLWLILFKLSFNLPQFLTYSYSNVNLVPF
                     STFSFENTTVLRETTYNLIVFFPFGVLLNVNFKRLSFSKKLGIIFLVSFLAELIQFLF
                     GIGVADITDLITNTTGGLIGLWAYQLLNKHLSTNKLDRLAIILGYILFLFLIVAFLFL
                     TRTVRLQ"
     gene            1442..2467
                     /locus_tag="ACG842_002519"
     CDS             1442..2467
                     /locus_tag="ACG842_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFQ0249013.1"
                     /translation="MFKTYFRFNKIQDNPFRIMNLMLLIFIVLWLGYINHDFLISSFS
                     SLGIFTFFYYQNIPMKYLLKRMFLIGCGLLVAFVLGILSTYVLWLEPFAVALVAFSSR
                     FVLRLFHISKPGGLFFAMLSAMGTSMQLPIAQLPIVSLYFFMGVVFALIAAVITKLLD
                     SRPEQTIEKATLKERFHEEPLVIIDSVFYSAALFLSVYVSHGLNLHNPYWLTLSCASI
                     LLAENLDAMKHRQVQYLIGSMGGLCVSAFLSFVPFTQLQTIFLITFLYGIAQFLVARN
                     YAVANIFLNPMALMLSTLIRGAYLISLIEYRFLGIVIGSFIGLGVAWVMTVGLQHYLT
                     VVRQKLE"
     gene            2790..3668
                     /locus_tag="ACG842_002520"
     CDS             2790..3668
                     /locus_tag="ACG842_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase domain-containing protein"
                     /protein_id="HFQ0249014.1"
                     /translation="MRFKDMKRGNLMKKISNEIQILAETLTLPKVVIEQMIVQAKNIL
                     DEVFEQYSHELMDPNKAKATAKKIDKVYEEEPFAALAIYLYAASQSWKKIYQPLKIPR
                     TIYLATMNAFTRFIHEHFQVTGSYRFDRGFWIWHYTSGLIFRIGELEYEMTYFPKGYT
                     DLRLEGKQCLSLHIPSDADLSQNKIRDSYESAIRFFELYFPDYHYQVMYTDTWLLSPN
                     LTKWLKKESKICLFAADYRLLSVDEQDDSGVPWIFGRVDAQIHDYPESTSLQRQAKEQ
                     LLAGEHIGSGLGILTS"
     gene            4144..4260
                     /locus_tag="ACG842_002521"
     CDS             4144..4260
                     /locus_tag="ACG842_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HFQ0249015.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKDVKRNSGKR"
     gene            5188..5526
                     /locus_tag="ACG842_002522"
     CDS             5188..5526
                     /locus_tag="ACG842_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0249016.1"
                     /translation="MTQPVSSEEHDNLFRATEEFKKYQENIVPETPLASEPITDIDGN
                     QIGWYIQYEVMVVNNDSIVKNTVGLELTPIEKVSSLLTYIYTDESDETEIILTDYSKI
                     ATDKKYILWM"
     gene            5620..5850
                     /locus_tag="ACG842_002523"
     CDS             5620..5850
                     /locus_tag="ACG842_002523"
                     /inference="COORDINATES: protein motif:HMM:NF042636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6568 family protein"
                     /protein_id="HFQ0249017.1"
                     /translation="MLVEKGKENIYYVNVAKVREDENEWKEFKSRYSINSTPTFTVYR
                     EGSIEKTVFWTKESGMSLAEVEEFLDYVSMQQ"
BASE COUNT         2039 a          894 c         1095 g         1965 t
ORIGIN      
        1 taaatattaa cgtgttttca aacagtataa aacggtgtgg tttttggaat aaaaaaataa
       61 catatctcat tttacacttt tgttatttta tacttaaaaa ataacgacac agaaaatacc
      121 gtgtctcaga tttctctact acttctaaaa actactgttt ttttcctttg aaatgccttc
      181 aaaaagtagg atcaggcaga atatactggc agaatcaagg agaagggagc ctaaataagg
      241 agaaaaacca tagaaaatat aaagtaatta cttataaaac aagcttttta tagaaaaata
      301 atatacctga gtagggtata aattaagacg gaattccttt ctagattgag atattgacta
      361 ggaagaatgt taagatttta agcctttaag ctaaaaaaaa accctttttg ttaaataagg
      421 ggaatatagc ctgtcagttt caaactgcaa gtcgtcctgc tgcaataata tagtagatta
      481 gagatgaaaa tagatttttt taaaccaatg actgaataaa aagaccgtca tagatacaca
      541 ttggaatgtg tctattacgg tcttttttta cgaacgattt tattgaagac ggacagttcg
      601 ggtcaaaaat aaaaaagcta cgatcagaaa taagaataaa atgtatccca aaataatggc
      661 tagacggtct aacttatttg tcgatagatg tttgtttaga agttgatagg cccataaacc
      721 tatcagtcct cctgttgtat tagttatcaa atcagtgatg tccgctacgc caataccgaa
      781 taggaattga atcaattctg ccaagaagct tacaaggaaa ataatcccta attttttaga
      841 aaaagaaagt ctcttgaaat tgacattcaa aagcacccca aaaggaaaaa agacaatcaa
      901 gttataagtg gtctcacgta ataccgtcgt attttcaaaa gaaaaagtag aaaaagggac
      961 gagattgaca ttgctgtaag aataagtgag aaattgtgga agattaaatg atagcttgaa
     1021 taaaatcaac cataatagaa agaataaata aatccctaat aaaaattttg atacataccg
     1081 catagacatc tcctccattt aaaaagtata ccatcgaaaa ccgctgaaaa atcataaaaa
     1141 agtatgaagt ttaaggacca acgtagttat cgtacgaaaa ttagaagctt ttttttgttg
     1201 ggaatccggt ttctttaaca aagctaagat agcgagttgc ttggaaaagt tgctatcttt
     1261 tttctgtcaa aacgactcgg aatgttcgtt ttttgttcta atttctccaa gttgtttact
     1321 ttttgatact aagtttatga aaaatcaaga aatagttgtt atactaggta atgctgacaa
     1381 acgggattgg catttcatct tggttcagct ttttttccaa ttactaggaa ggagggatag
     1441 attgttcaag acttattttc gtttcaataa aatacaagat aatccttttc gtatcatgaa
     1501 tctgatgcta cttatcttta ttgtattatg gttaggttac ataaatcatg attttttgat
     1561 ttcaagtttt tcatcattag ggatctttac ttttttctat tatcaaaaca taccgatgaa
     1621 atatttgctg aaacgtatgt ttctgatcgg ttgcggactg ttggttgctt tcgttttggg
     1681 gatcttatcg acttatgttt tatggcttga accatttgcc gtagctttgg ttgctttttc
     1741 cagcagattc gtgttgcgat tattccatat ttctaaacca ggtggcttgt tttttgcgat
     1801 gctttctgcc atgggaacga gcatgcagct gccaatcgct caattaccga tcgtctcact
     1861 ttattttttt atgggtgtcg tttttgccct gattgcggca gtgattacaa aattactgga
     1921 ttcacggcct gagcagacaa ttgaaaaagc aactttaaaa gaaagatttc acgaagagcc
     1981 tttagtgatc attgattctg ttttctacag cgctgcctta tttctttccg tttatgtcag
     2041 tcacggcttg aatctgcata atccttattg gttgaccttg tcatgtgcat cgattttgct
     2101 agcagaaaac ttggatgcga tgaagcatcg acaagtccaa tatctgattg gatcgatggg
     2161 tgggttatgt gtgtcggcat tcttgtcatt tgtgccgttt acgcagttgc agaccatttt
     2221 tctgatcact tttctatatg gaatcgcaca atttctagtt gctagaaatt atgcagttgc
     2281 taatattttt cttaatccga tggcgttgat gctatctaca cttattcgtg gagcatactt
     2341 gatttctttg atcgaatatc gttttttagg gatcgtcata ggaagtttca tcggtttagg
     2401 tgttgcgtgg gtaatgacgg ttggtttgca acattattta acggttgttc gtcaaaaatt
     2461 agaatagaag gacaaatata tgtgccaaaa gaggttatga caagattttt gtcacaacct
     2521 ctaattcaag gtgttcaaaa gccagattct cagcattaag tcaaattcac acaaaaatgt
     2581 ggaaaggaac tttcaaaaaa attttatatt tattctatca aaaaattttg tttatgatgg
     2641 taactgaata aactttcctg agatggtcaa tgctttcctt gaaagtctta cagatattct
     2701 ttcttcatag aaagtttaag ttttatagtt agtgtacaat aaaagtatgt ttataccata
     2761 tggtgtccgg ataatcagtc atttaaacaa tgaggttcaa agatatgaaa aggggaaatt
     2821 tgatgaagaa aataagcaat gaaatccaaa ttctggcaga aactttaact cttcccaaag
     2881 tcgttataga gcaaatgatt gtacaagcaa aaaacatcct tgatgaggtg tttgagcaat
     2941 atagtcatga actaatggat cctaataaag caaaggcgac tgcaaagaaa atagacaaag
     3001 tctatgaaga ggaacctttt gcagcattag ctatttatct atatgcagct tctcaaagtt
     3061 ggaaaaaaat ttatcaacca ttgaagattc caagaactat ctatttggca acaatgaatg
     3121 cttttactag atttattcat gaacatttcc aagtaacagg aagctatcgc tttgaccgtg
     3181 gtttttggat ttggcattac actagtggct tgatctttcg gattggtgaa ttggagtacg
     3241 aaatgacata ttttccgaaa gggtacacgg atctaagatt agaaggaaag cagtgccttt
     3301 ctcttcatat tccttccgat gcagatttat ctcagaataa gataagagat agttacgaaa
     3361 gtgccattcg tttttttgaa ctttattttc ctgattacca ttaccaagtg atgtacacag
     3421 atacttggtt actatcaccc aatcttacta aatggttgaa aaaagagtca aaaatctgtc
     3481 tttttgcagc cgattatcga ttattatcag ttgatgaaca ggacgatagt ggagtccctt
     3541 ggatctttgg acgagtagat gcccaaatac atgattatcc agaaagtacg agtttacagc
     3601 gacaagcaaa agagcaattg cttgcaggcg agcacattgg ctctggctta ggtattctta
     3661 cttcataagt ttagtacttc gaaatagttt aaaagagcgt gagacaaaag taaagaacac
     3721 ttttgtctca cgctctaaac acgtataaac ggcggaagca gaagcaactc cttcggaaat
     3781 aagctgaaat tcacaaaaat ttgaaaagca attttcgtga atttcttctt atttcttgga
     3841 gttaaacgct tctgtcccag cctctttttt agtgttctat cgaacacaca gtacgttcaa
     3901 taggtaaact agctataaca atagatatta aagttaacgg acaagagggc cttttttagg
     3961 tagctaaaac gtaataatga aaatttattt ccagagatag gagtcaatca gctagtctga
     4021 tcgactgttt taacaagttg actttcattt taacttttga taccatttaa aacacaaatc
     4081 gtaattgttt cgatttgtgt tttttagcat gactacaatc aaagaagaaa gtgggtcgtc
     4141 catatggaaa aaacagatat ctcaagtgca taccgccgct tgaaaagccc aaatatcaaa
     4201 acacgaaaac gtgcgctgaa gatcatcaaa gatgtgaaac ggaatagtgg gaaaaggtaa
     4261 gaagaaggaa acttaattaa aaaaatagcc gaaaaacgtt tttcggctat tttcaataat
     4321 cggtttttgt gtaaggtttc cgaaagaact gctgttgatt ttccattttt aacatatgat
     4381 aaattggtag aatctttctt ttcaaattga taccattccc taattccagt tatatagtta
     4441 actacagaac cacaaagcac gtacgtttat aaaagtatta tctgtagtta tttttcaatt
     4501 ccctttcaac ctcttatttt gaccagtagt attgatcact ttacctttta acacctttgt
     4561 tcgtttttga aacattttta gtcaagaatg gaaacgtaac tacataatca aaggttttgc
     4621 gaaatatttc atgaaatatt tcatgtagaa gcgagcaata cggttgcaaa taaccattat
     4681 cgtttaataa gcagttacga aagctcccaa tatctctaac gttcagctca ctccatttct
     4741 tttcttcaaa aaaatcagtg attcgtatct ctttctattt gtcgaaaaag ttaactttaa
     4801 aaagtaacgc cgaaaaaata tgcaaaaaaa taaaaaagca gttactattg ctagaataac
     4861 atcaaggaat aataagtttt ggttttcaga taatagtcga taagttgtgt attggaattt
     4921 tagatacagt aataaagtat ttttcatgaa attttgtgag aatcactatt acaagtatct
     4981 ttaataaaat taatggttaa ggaggaactt aaagtagaat gtaaatgaat agttgtagtc
     5041 agtcgattgt gatactagac tcaaagttgc aggaataagt ttttcaattt gtgattggaa
     5101 gataaaaaat gaaaaagaag ggcttttttt tatactttgt tgattgtagt gtttattttc
     5161 cctctgttta gccaagtaac attttctatg actcaaccag tttcaagtga ggaacatgat
     5221 aacctttttc gtgctacaga agagtttaag aaatatcagg aaaatattgt tccggagact
     5281 ccattagcta gtgagccaat tactgatatt gatggaaacc aaataggatg gtatatacag
     5341 tacgaagtta tggtggtaaa taatgactca atagtaaaaa atacagtagg cttagagcta
     5401 acaccgattg aaaaggtgag ttcattgtta acgtatattt atactgatga aagtgacgag
     5461 actgagatca ttttaacgga ttactcaaaa attgctactg ataaaaagta tatattgtgg
     5521 atgtagacac tagtgaaata ctttatgata tagaaattga tgaaaattta ctagatgtag
     5581 cacaagaaat cgatcatcaa aagcaggaag ctattcgata tgctggtaga aaagggaaaa
     5641 gaaaatattt attacgtgaa tgtagcaaaa gttagagaag atgaaaatga gtggaaagaa
     5701 ttcaagagtc gctactcaat taactcgact ccgacattta cagtttacag agaaggatcg
     5761 atagaaaaaa ctgttttttg gactaaagaa agtggaatgt ctttagctga agtagaagag
     5821 tttttggatt atgttagtat gcaacagtaa gggagttctt attatgaaaa attttgacac
     5881 atattatgtt attgtttttt cgatatcttt gctgttaata ttactaactt taatgcagta
     5941 taaagagtat ccagagtatt ttattttaac ttttgtatat ttagtaagta gct
//
LOCUS       DAZKLM010000121         5972 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3764
            SAMEA115706913-rid22582563.denovo.126, whole genome shotgun
            sequence.
ACCESSION   DAZKLM010000121 DAZKLM010000000
VERSION     DAZKLM010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706913
            Sequence Read Archive: ERR13196647
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5972)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5972)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287823.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287823.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,004
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,849
            CDSs (with protein)               :: 2,849
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 41 of 96
            Pseudo Genes (incomplete)         :: 52 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 15 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5972
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706913-rid22582563.denovo.126"
                     /isolate="VRE3764"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..538
                     /locus_tag="ACG842_002524"
     CDS             <1..538
                     /locus_tag="ACG842_002524"
                     /inference="COORDINATES: protein motif:HMM:NF024700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFQ0249018.1"
                     /translation="LDIRVSGESSIFSDKKLINSIRKLNDAQVLELQTLKPNDLVVIR
                     LELNGKKVSLSNASAGQKTSAILSMILAYGNSPLILDQPEDDLDSQLINNLIVRSIIT
                     KKENRQILIVTHNANIPVNGDSEWLISMGDSKEISADQCGSVDEPNIKPRICTVMEGG
                     EDAFTNRAKRYGFKQIVE"
     gene            588..977
                     /locus_tag="ACG842_002525"
     CDS             588..977
                     /locus_tag="ACG842_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFQ0249019.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLLFV
                     IVFAHVPPLSMIEEVFLKYLGIPVAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPSTLVRSVNYVSD"
     gene            964..2463
                     /locus_tag="ACG842_002526"
                     /pseudo
     CDS             964..2463
                     /locus_tag="ACG842_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            3211..5037
                     /gene="ltrA"
                     /locus_tag="ACG842_002527"
     CDS             3211..5037
                     /gene="ltrA"
                     /locus_tag="ACG842_002527"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HFQ0249020.1"
                     /translation="MLKKNKLRYNEYYDMQHIYDELYAQSKNGNNFYKLLEIIGSEQN
                     ICLAYRNLKSNSGSKTAGTDGMTIDDIKQLSNAEIVATVRESLSNYRPKSVRRVFIPK
                     AGSDKMRPLGIPCIWDRLVQQCILQVLEPICEPKFHNHSYGFRANRSAHHAVSRVTTL
                     INLSKYHYCVDVDIKGFFDNVNHGKLLKQIWTLGIRDKRLICIISKMLKAEIDGEGVP
                     EKGTPQGGLLSPLLSLIVLNELDWWVSSQWETFQPKNRSKNGWLQYAKKYTKLKSGFI
                     VRYADDFKIMCSTYGEAQRFYHSTVDFLNKRLKLEISPEKSKVVNLKKNSSDFLGFKI
                     KVIPKGRTKHGYVAKTDMNQKALKKAKTNLKLKVKDIARHTTGFNISRYNLTVIGMQN
                     YYCIATNVYNNLTEVSYALLPTIRIRLRNIAKSVPFESTSSEFQSRTKGIRPKTKIVM
                     IADNPLLPIQGVQHKNPMNFSQDICNFTKQGRNKVHEDVVVVTKEEIRALLENENPAD
                     SVEFNDNRISAYIAQQGNCYVMNRRGTPSTLICIHKSDKGDNLDRYSNLAFVEIPIAK
                     AILTESADEAKSLLKGYVLNSQQKKKLNRIRTNYGYQTITGK"
     gene            5150..5972
                     /locus_tag="ACG842_002528"
                     /pseudo
     CDS             5150..5972
                     /locus_tag="ACG842_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
BASE COUNT         2025 a         1016 c         1300 g         1631 t
ORIGIN      
        1 acttgatata agagttagtg gggaaagtag tattttttca gataaaaaat taatcaattc
       61 tataagaaaa ttgaatgatg cacaagttct tgaattacaa acccttaagc caaatgattt
      121 agtagttatc cgacttgaac taaatggaaa aaaagttagt ttatcgaatg ctagtgctgg
      181 tcaaaagaca tctgcaattc tttctatgat tttggcgtat ggaaattcac cactcatatt
      241 ggatcagcct gaagatgatt tggatagtca attaattaat aacttaatcg taagaagtat
      301 cattacaaaa aaggaaaaca gacaaatttt aattgtaact cataatgcaa atatcccagt
      361 taatggtgat tcagaatggt tgattagcat gggagattca aaagaaatat cggctgatca
      421 gtgcgggtcg gtagatgaac ccaatatcaa gccaagaata tgtactgtga tggaaggtgg
      481 cgaggatgct tttacaaata gagcgaaacg ctatggtttt aaacaaattg tagaataaca
      541 ctagagggtg agacatatta ttatgtccgc cctttttagg aggaaaaatg aaaaaaataa
      601 aaagctatac cagtatctgg gcagttgaaa aagtcattta cgcaatcaat gattttcagt
      661 tgccttttcc tgtgactttt aaccaaatgg cttggttcgt tttatcttta ctgtttgtca
      721 tcgtatttgc ccatgtgcct ccactatcta tgatagagga ggtcttttta aaatacttag
      781 gcataccggt ggctgtcact tggtttatgt cgcaaaagac gtttgatggc aagaaaccac
      841 tgggattttt acgatccttt atcagctatc acttgcgatt taaggtgact tttgcaggaa
      901 agaaagtcaa agaacagaag aaacgatggg atgaacccag tactcttgta aggagtgtga
      961 actatgtatc cgattaagta tattgaaaat aatctcgtct ttaaccaaga aggcgagtgt
     1021 tttgcctact atgaattagt gccttacaat tactcctttt tgtcacccga acagaaattt
     1081 caagtgcatg ataactttcg ccaactaatt gcgcaaaatc gtgaaggcaa gatccatgcg
     1141 ttacaaattg ccacagaaag tagtattcgc gccacacaag aacgatcaaa aaaagaaatt
     1201 accggtagac tcaaagaagt agcgaaacaa cgaattgata tacagacaga tgcattagta
     1261 tccatgattg gcgatagtca gattgattat cgctttttta ttggctttaa actaattgcg
     1321 acagatgaag aagtcaatct aaagagtctc aagaaatctt tcttttctgg gcttcaagaa
     1381 tttgtctatg gagtgaatca tcatttgatg ggcgattttg tttccttatc caatgaagaa
     1441 attcgctgct attctaagct tgaaaaatta atggaaagca aattggctcg aagatttaaa
     1501 gtcaggcgag tcacaccgag tgacctgacg tatttgattg aacatattta tggcgaaaaa
     1561 gggataccgt ttgaagaata cgaatttcag ctaccaaaga aaaagctaaa atcagaaaca
     1621 ctggtgaaac gttatgactt attacgtcca aatcgttgtt taattgaaga aaaaccaagg
     1681 tgcttacgga tggaacatga gaatcatgag tcgtacgtag cgtatttaac aattaataca
     1741 attgtggggg agatggaatt tccttcctca gaacttttct attaccagca acaacaattt
     1801 acattcccaa tcgacacaag tatgaatgta gagattgtga cgaataaaaa ggctttagct
     1861 accgttcgta ataagaaaaa agaactgaaa gatttagaca atcatgctta ccaatctgac
     1921 aatgaaacga attccaatgt cttagatgct ttggattccg tggatgaatt ggaaaccacc
     1981 ttggatcagt caaaagaatc tatgtacaaa ctgagttatg tggttcgagt gagtgccgaa
     2041 tcagtggatg aactaaagcg acgctgtgat gaagtgctcg atttttatga tgataccaat
     2101 gtaaaactag tccggccctt tggcgatatg atggggctac atgaagaatt cttaccctta
     2161 agtaaacgat atatgaacga ctatattcag tatgttacgt ctgatttttt ggcggggctt
     2221 ggttttgggg ctactcagat gctaggagaa ttagaaggga tttactttgg ctacaatgtg
     2281 gatacaggtc gtaatgttta cctgaaaccg gcattggctt cacaaggagt caagggttca
     2341 gtaaccaatg cgctggccgc cgctttcctg ggttctcttg gtggaggtaa atcctttagt
     2401 aacaatctct tagtctacta tgctgttttg ttcggtggtc aagctgtgat tgtcgatcca
     2461 aaagggggtg cgaaacgttc ctacttgaaa agagtaggca cagcacttca ttaaatcaat
     2521 atttaatgtg tgttggagaa ctaaacacac gaatagtcga atgacggggt aacgccctga
     2581 aaggctactt taatactctg actggcagaa acttgagtaa acaaggctct gtcagaagct
     2641 cggtgaagtc ggcagacaga caccgtaaca gataatgctg acgaaagatt cccagaggtg
     2701 ggcgttgtgt cctatatgcc ggaagtctat aaaatatcta tggttagaat gtagatgaga
     2761 ggtctactga cgaatccttg aatgtacagg tctatatcgg ggctggatag aaatgtccag
     2821 tgacacaaac agtgtcgcta ctgtgggtga gtaagactcc gcatttatga aaatccatat
     2881 aacgttaaag gcgttatcaa ggtagcaggc ttatagagga aacctaagga tatatgtaca
     2941 gatagtgtgt ttggaacgtt gaaagctgac aacatggaga ttttactgtc tgtgaagtgt
     3001 tcacatcaga aagttattgt gagattagca atgatataag gtgtgttaat gtcagtgaga
     3061 gtggtggcac agtacctatg aaaccgtgat aacaagcggt ggagggatag ccactagtca
     3121 tatgaagaat agaattacta agaaaaatgg tatgggttcg agtaagacta agagatgtac
     3181 ttgctcctgc aaacaaggag ggtacagacc atgctaaaga aaaacaagct ccgctataac
     3241 gagtattatg atatgcaaca tatttatgac gagttatacg cacagagcaa gaacggtaat
     3301 aacttttata agttacttga aattattggt tcggaacaaa atatttgttt agcttaccgt
     3361 aatctgaaat caaacagtgg cagtaaaacg gctggaacgg atggaatgac gatagacgat
     3421 ataaaacaac tcagcaatgc tgagattgtt gctaccgttc gggaaagcct tagcaactac
     3481 cgccccaaat cagtcagacg tgttttcatt ccaaaagctg ggagcgataa aatgcgtcca
     3541 ttggggattc cgtgtatctg ggacaggctg gtacaacaat gtatcctgca agtcctagag
     3601 ccgatatgcg aaccaaagtt ccataatcat tcctatggat tccgtgcgaa tcgaagtgcc
     3661 caccatgcag tcagcagagt aacgacactg ataaatttat caaaatatca ttactgtgtt
     3721 gacgtagaca ttaaaggatt tttcgataat gtcaatcatg gaaaactatt aaaacaaatc
     3781 tggacacttg gtattcggga taaaagattg atttgcatta tcagcaaaat gctgaaagcc
     3841 gaaattgacg gcgagggtgt cccagaaaaa ggaacgccac aaggcggtct gttaagtcca
     3901 ctgctctccc tcatagtcct taatgaatta gactggtggg taagcagtca gtgggaaaca
     3961 ttccagccga agaaccgcag taaaaatggg tggcttcaat acgcaaagaa atataccaaa
     4021 ctgaaaagcg gttttattgt acgttacgct gatgatttta aaatcatgtg ttcaacctat
     4081 ggagaagcac aaagatttta ccacagtacc gtagattttc ttaataaaag gctgaaatta
     4141 gaaatcagtc cagagaaatc aaaggtagta aatctgaaga aaaattcatc agattttctg
     4201 gggttcaaaa tcaaagtcat tccaaaagga agaacgaaac acggttatgt tgccaaaaca
     4261 gatatgaacc aaaaagcatt aaagaaagcg aaaacaaatc taaagttaaa ggtaaaagat
     4321 atagccagac atacgactgg cttcaatatc agcagatata atctgacagt tatcggtatg
     4381 cagaattact actgcatagc aactaacgta tataacaacc tgacagaagt aagttacgct
     4441 ctcttaccga ccatacgaat ccgtttaagg aacattgcaa aatcagttcc atttgaaagt
     4501 acaagttcag aatttcagag caggacaaaa ggaatcagac caaaaacaaa aatagtgatg
     4561 attgcggata atccgctatt gccgattcaa ggagtacaac acaaaaatcc tatgaatttc
     4621 tcgcaagata tttgtaattt tacaaagcaa gggagaaaca aggtgcatga ggacgttgta
     4681 gttgtaacaa aagaagaaat ccgagcgttg ttggaaaatg aaaaccctgc cgatagcgta
     4741 gagtttaacg acaatcgtat ttcggcatat atcgcacaac aaggcaactg ctatgtaatg
     4801 aatcgtcgag gaactccgtc cactctgata tgtattcaca aatcagacaa aggagataac
     4861 cttgacagat attctaatct ggcatttgtg gaaattccca ttgcaaaggc aatcctaaca
     4921 gaatcagcag acgaagcaaa atcactgtta aaaggttatg ttcttaatag tcagcagaag
     4981 aaaaaactga acagaatacg gacgaattac ggataccaga caataactgg gaaataactt
     5041 ataaatctat ttggatatga tggaacgccg tatgaagcga aagtttcatg tacggtgtga
     5101 agcgggggaa aacttggaga taacatcaaa aagttaccta tcgctatagc gaggagggtg
     5161 gaaagaaacc ttgccagaaa ttgcggatga aatcagtatc ctcaacttga cgagtaagtc
     5221 agaaaatcaa ggtctgcttg atccttatgt gattatgaag aaaaagaaag actcggaaag
     5281 tttagcaatt gatattctga cttttttaac aggaatttca agtcgagacg gggagaagtt
     5341 tcctgtgtta cgtaaagcca ttcgaacggt gactcaaagt gatcaacggg ggttattaca
     5401 agttattaac gaattgcgaa aagacgggtc accaatcgcg gaaaatatcg ccgatcatat
     5461 cgagtccttt accgattatg attttgctca tttgcttttc tcagacggct cgattaccca
     5521 atcgattagt ttagataaac agctaaatat cattcaagtg gcggatttgg ttttaccgga
     5581 tgcagaaacg acttttgaag aatataccac catggaattg cttagtgttt cgatgctaat
     5641 cgtcatttcc acttttgcct tggactttat ccatagtaac cgagaaatct tcaaaattgt
     5701 cgatttggat gaggcttgga gttttcttca agtggctcaa ggaaaaacgt tatcgaataa
     5761 gcttgttcgt gctggtcggg ccatgaatgc tggggtctat ttcgtcactc aaaatagcga
     5821 cgatttatta gatgaacgtt taaaaaataa tatcgggttg aagtttgctt ttcgcagtac
     5881 cgatattcat gaaatcaaga aaaccttgga attctttggt ttggatcagg aggatcaaag
     5941 caatcaaaaa cgcctgcgag atttggaaaa cg
//
LOCUS       DAZKLM010000122         5913 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3764
            SAMEA115706913-rid22582563.denovo.127, whole genome shotgun
            sequence.
ACCESSION   DAZKLM010000122 DAZKLM010000000
VERSION     DAZKLM010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706913
            Sequence Read Archive: ERR13196647
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5913)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5913)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287823.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287823.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,004
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,849
            CDSs (with protein)               :: 2,849
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 41 of 96
            Pseudo Genes (incomplete)         :: 52 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 15 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5913
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706913-rid22582563.denovo.127"
                     /isolate="VRE3764"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(139..597)
                     /locus_tag="ACG842_002529"
     CDS             complement(139..597)
                     /locus_tag="ACG842_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HFQ0249021.1"
                     /translation="MHFFKTRNILKAVASGQIIPLTAVKDEVFSSKMIGVGYAITNHD
                     GYIHAPVKGIIDNIFPTRHAITIESESGRKILIHMGLDTVELEGSPFQIRVESNQEVK
                     EGTLLAIIDLKKLEQAQKDTVIIVVSPGIFEGNLLKQNQEVKIKDSCFEF"
     gene            complement(610..1941)
                     /locus_tag="ACG842_002530"
     CDS             complement(610..1941)
                     /locus_tag="ACG842_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose-6'-phosphate glucosidase"
                     /protein_id="HFQ0249022.1"
                     /translation="MMMKKQIITIAGGGSTYTPGIVQAILNNEKRLPLSEIRLYDIDE
                     ERNMDMYLIVKFMLKRKGFSNIRIRATDDPKLAFTGCDFVFSQIRVGGLEMREKDEKI
                     PLKHGLVGQETCGLGGFAYGMRSIKGLLEIVDHIQDYAPKAWILNYTNPESIVSEAVR
                     RKYPNVKMINACDMTISIEETIAVNYGYDRKNWIVTYYGLNHFGWYTSIYDKELQREI
                     MPEIIEKLITKEMQVADFNIGDKTWQKTFQMMSVITKNFPSNIPNNYLEYYLYPDMVV
                     EHTDKEYTRANMVMDGREKNTKEMADKIRRGIVEEVLNFNFGEHGQYIVDIAISLLND
                     DRRRFMLIVPNQGAIPNLRSDAVVEIPAYVGATGVEPITLRKPISDFHKGLMEAQVAV
                     EKLLVDAYFEGSYQKALQAFTLNQTVPNARVAKKILDEMIKVNKKYWPALK"
     gene            complement(1952..3394)
                     /locus_tag="ACG842_002531"
     CDS             complement(1952..3394)
                     /locus_tag="ACG842_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFQ0249023.1"
                     /translation="MRIFNRGGIKMNVKSNVQKLGRAMLLPVAAMPLAGLIMRLSADD
                     MLNIPVIGAAGNAVFGNLDILFAIGVAIGFVKNKDKGIPALTGFLAIATLKKGLEIMD
                     PEVNMGIFGGIISGLVAAWTYNRFKEQKLPVVFSYFAGEKFPLTMVMILQTIAAVFLG
                     FIWPLIQIGIDNFAKLLVDMGALGVGIFMFLNRLLIPFGLHHVLNTYIYYDLGSYTSP
                     SKEVFRGEMTRFINGDPQAGLFLSVFFVVMMFGIPAICLAIYKAAYKENKEMVKGIMG
                     SGAGTSFIANITEPVEFSFMFLSPMLYVIHALFAGLAGIVCYLFNIRIGFTFGACIVD
                     YLINFRIATNAILILPIGIFFFALYYVTFYYLINKRNIQTLGREAKAEFGNEVTLEET
                     ELGLASKNYEYMATKMLQAFGGKANILDVYSCNTRLRVEVVDPTMVEEQRIKQLGISG
                     IIKPTEKNYQIIIGLEVTYVMAEFNKLLEE"
     gene            complement(3381..4145)
                     /locus_tag="ACG842_002532"
     CDS             complement(3381..4145)
                     /locus_tag="ACG842_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate deacetylase"
                     /protein_id="HFQ0249024.1"
                     /translation="MGKVIFNSDDFGYSHGVNYGIMDAYQRGILTSTTLMANMPGFEH
                     AVKLRKEMPRLGVGVHLTLTCGKPLLKTVDTLIESGEFRSLSYYQHPFNIDKDQLYQE
                     WNAQIQKIYHAGIIPTHLDSHHHTHTLDENQEVVVTLAKKYDLPVRSNFERKDEVRHV
                     DYFEPCFDVVGETDEKKRQINTSVEEYLASLLMILREDKTIEVMCHAAYLDQELLNGS
                     SFVYPRTHQVEFLIHSAFAQKVKNDPLIELVTYEDI"
     gene            complement(4249..5124)
                     /locus_tag="ACG842_002533"
     CDS             complement(4249..5124)
                     /locus_tag="ACG842_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFQ0249025.1"
                     /translation="MKESASCFVLSYRGGELTVYVMKKLNHNAVLIDDDGIEKIAMGK
                     GIGFSAKVSKPFDVEDADKLFVLDSKEKIKRFSEIVSQIPIEFMDFAEEVIELITARI
                     SSPLDSNIYITLTDHIYFAIQRKREDRDVTAIMLPEMRFLYPNEYDIAVEVVERINHR
                     YQTELGSNEVGFITMHIINAELGEPNSLKSLKIVEMTTTILSHLEKSYSNVYDKESLT
                     YHRLMVHIKFLVKRLIYQEETPVENIGFFTETFKHSKAYCTALKIKDIIEQQFQLAIQ
                     ENEIIYLAIHLARIY"
     gene            complement(5379..5463)
                     /locus_tag="ACG842_002534"
     tRNA            complement(5379..5463)
                     /locus_tag="ACG842_002534"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5427..5429),aa:Leu,seq:caa)
     gene            complement(5481..5552)
                     /locus_tag="ACG842_002535"
     tRNA            complement(5481..5552)
                     /locus_tag="ACG842_002535"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5518..5520),aa:Gln,seq:ttg)
     gene            complement(5562..5634)
                     /locus_tag="ACG842_002536"
     tRNA            complement(5562..5634)
                     /locus_tag="ACG842_002536"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5599..5601),aa:His,seq:gtg)
     gene            complement(5696..5780)
                     /locus_tag="ACG842_002537"
     tRNA            complement(5696..5780)
                     /locus_tag="ACG842_002537"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5744..5746),aa:Leu,seq:caa)
     gene            complement(5801..5871)
                     /locus_tag="ACG842_002538"
     tRNA            complement(5801..5871)
                     /locus_tag="ACG842_002538"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5837..5839),aa:Cys,seq:gca)
BASE COUNT         1815 a         1257 c          898 g         1943 t
ORIGIN      
        1 aatcatagga gcaacaaaaa aattcgataa ttaacaaaaa tcaaaatgat taaatctagt
       61 gattgaaaca tatcatttca aaaaaatttt gtaaattcac ccataaatat caccctattt
      121 aacttttttc atcgtacact aaaattcaaa acaactatct ttaatcttta cttcttggtt
      181 ttgttttaat aaatttcctt caaaaatgcc tggagaaact acaatgatca ccgtatcttt
      241 ttgtgcttgc tccaacttct tcaaatctat aatagctaac agtgtccctt cttttacctc
      301 ctggttactt tcaacccgta tctgaaacgg acttccttcg agttcaactg tatctaaacc
      361 catatggatc agtatttttc tgccagattc actttcgatc gttatggcat gtcgggtagg
      421 aaatatatta tcgattattc ctttaactgg cgcatggata tatccatcat gattcgttat
      481 agcgtatcca actcctatca tttttgaaga aaagacctcg tcttttactg ctgttaatgg
      541 aataatttga ccactagcta cagcttttag aatgtttctt gttttaaaga aatgcacttt
      601 ttatcacctc tactttaatg ctggccaata ttttttgttt actttgatca tttcatctag
      661 tatttttttt gcgactctgg catttggtac ggtttgattc aatgtaaatg cttgtaaagc
      721 tttttgataa gaaccttcaa agtaagcatc tactagtaac ttctctactg caacttgtgc
      781 ctccatcaat cctttatgaa aatcactgat tggttttctt agtgtaattg gctccacacc
      841 agttgcacca acatatgccg gtatttcaac aactgcatct gaccgtagat tcggaatcgc
      901 cccttgatta ggaacgatta gcataaagcg acgacgatca tcattcaaca aggaaatagc
      961 tatatcgacg atatattgtc catgctcccc aaaattaaag ttcaatactt cttccacaat
     1021 accgcggcgt attttatcag ccatttcttt ggtatttttc tctcgtccat ccatgaccat
     1081 attagcacgt gtatactctt tgtcagtatg ctcaacgacc atatcaggat ataagtaata
     1141 ctctaagtaa ttattcggga tattacttgg aaaatttttc gtgatgactg acatcatttg
     1201 aaaggttttt tgccaagttt tatctcctat attaaaatcg gcgacttgca tttcttttgt
     1261 tattagtttt tcaataatct ctggcataat ttctcgttgc aattctttat cataaatcga
     1321 tgtataccag ccaaagtggt ttaaaccata atacgtaacg atccaattct tacgatcata
     1381 accgtaattc actgcaatcg tttcttcaat tgaaatagtc atatcacagg cattaatcat
     1441 ttttacattc ggatatttac gacgaacagc ttcagaaacg atcgactctg gatttgtata
     1501 attcaaaatc catgctttgg gggcatagtc ttgtatatga tcgacaattt ccaataaacc
     1561 tttgattgag cgcatcccat aagcaaatcc tccgagtcca cacgtttctt gtcctactaa
     1621 tccatgttta agtggaatct tttcgtcctt ttctcgcatc tccaatcctc caacacgaat
     1681 ttgtgagaaa acaaaatcgc atcctgtaaa agcaagcttt ggatcatccg ttgcacgaat
     1741 gcgaatattt gaaaaacctt ttcttttcag cataaattta acaatcaagt acatatccat
     1801 atttctttct tcatcaatat catataaacg tatttcagat aatggcaacc gtttttcatt
     1861 gttcaaaatg gcttgaacga ttcctggtgt atacgtactc ccgcctccag ctatagtgat
     1921 aatctgtttt ttcatcatca tcttttcctt tctattcttc taataattta ttgaattctg
     1981 ccataacata agtcacttct aagccgatga taatttgata atttttttca gttggtttaa
     2041 tgatcccgct tatccctaat tgcttgattc gctgttcctc gaccatggtg ggatccacta
     2101 cctctacccg taggcgtgta ttacaactat agacatccag gatattggct tttcctccaa
     2161 acgcttgtag catttttgtt gccatatatt cataattttt acttgctaac cctaactctg
     2221 tttcttctaa ggttacttca ttgccaaact ctgccttagc ttcacgtcct aatgtctgaa
     2281 tgtttctctt attgatcaaa taataaaaag taacgtaata caaagcaaaa aagaaaattc
     2341 cgattggaag aatcaaaata gcattggtag cgattcgaaa attgatgaga taatcaacaa
     2401 tacatgctcc gaatgtaaaa ccaattcgta tattgaataa gtaacagaca atccctgcta
     2461 aaccggcaaa aagtgcatgg atcacgtaca acataggaga aagaaacatg aaactaaatt
     2521 caacaggctc agtgatattt gcaataaatg aagttcctgc accgcttccc atgattcctt
     2581 tgaccatctc tttattttct ttataagctg ctttatagat agctagacat atagctggta
     2641 taccaaacat cattactaca aaaaaaactg ataaaaacag tccagcctga ggatctccgt
     2701 tgataaagcg tgtcatctct cctctgaaaa cttccttgct aggagatgta tagcttccta
     2761 aatcgtaata aatatacgta ttcaacacat ggtgcagtcc aaaggggatc agcaaacgat
     2821 tcaaaaacat aaatatcccc acacctaatg ctcccatatc aacgagcagt tttgcaaaat
     2881 tatcaatccc aatttggatg agcggccaga taaagcccaa aaaaacagct gcgattgttt
     2941 gtagaatcat caccatagtc aatgggaatt tttcgcccgc aaaataagaa aaaactactg
     3001 gtaatttttg ttctttaaag cgattatacg tccatgccgc tactaaaccg gaaataattc
     3061 caccaaaaat ccccatattc acttctgggt ccataatttc taagcctttt ttcaaggtag
     3121 caatcgctaa aaaaccagta agtgcaggta ttcctttgtc cttatttttg acaaaaccga
     3181 ttgcaactcc tatagcaaat aagatatcca agtttccaaa aacggcattt ccagctgcac
     3241 caattactgg gatgttcagc atatcgtcag ctgataaacg catgatcaat cccgctaatg
     3301 gcatcgctgc aactggaagt agcatcgctc ttccaagctt ctgcacattt gatttaacat
     3361 tcatttttat tcctcctcta ttaaatatcc tcatatgtta ctaactcaat caaaggatca
     3421 tttttcactt tttgagcaaa tgctgaatga attaaaaatt cgacttggtg ggtacgagga
     3481 tacacaaaac ttgaaccatt taacaattcc tgatccaaat acgctgcgtg acacatgact
     3541 tcgatcgttt tatcttctct caatatcatg agtaaacttg ccaaatattc ttccacactc
     3601 gtattgattt gtcgtttttt ttcatcagtt tctcctacga catcgaaaca aggctcaaaa
     3661 taatcgacat gacgaacttc atcttttcgt tcaaagttgc tccgaaccgg caaatcatat
     3721 ttttttgcta atgtaacaac tacctcctga ttttcatcca aagtatgagt atgatgatga
     3781 ctgtctaaat gagttggtat gatccccgca tgataaattt tttgaatctg agcattccat
     3841 tcctgataca gttgatcttt gtcaatgtta aatggatgct gataataaga taaggatctg
     3901 aactctccgc tttcgataag agtatcgaca gttttgagga gtggtttgcc acacgtcaat
     3961 gttaaatgga ctcctacacc tagcctaggc atttcctttc ttagtttcac agcatgctcg
     4021 aaacccggca tatttgccat caatgtagtt gatgtcaaaa ttcctcgctg ataagcatcc
     4081 ataatcccat aattcacacc atggctatag ccaaaatcat ccgaattaaa aatcactttt
     4141 cccatttcat tctccttctt ttttctacta aaataggcgc aaccaaataa tcatagtcca
     4201 tttactatgt gtcatttggt tgcgcctata gatagttacg cccttatttt aatagattct
     4261 tgctagatga attgccaaat agatgatttc attttcttga atggcaagtt gaaattgctg
     4321 ttcgatgata tctttaatct ttagtgctgt acaatacgct ttgctatgtt taaatgtttc
     4381 agtaaaaaaa ccaatatttt ctactggcgt ttcttcctga taaatcaagc gtttaaccag
     4441 gaatttgata tgaaccatta agcgatgata agtcagtgat tctttatcat aaacgtttga
     4501 ataggatttt tctagatggg acaaaatcgt ggtcgtcatt tctactattt ttaagctctt
     4561 taaactatta ggctctccta gctcagcatt aatgatatgc atagtgatga atcctacttc
     4621 atttgaccct aactcggttt gatatcgatg attgattcgc tcgaccactt ctactgctat
     4681 atcatattca tttggataaa ggaatctcat ttctggcaac ataattgctg tgacatctct
     4741 atcttctctt tttcgttgaa tagcaaaata aatatgatct gtcaatgtaa tatagatatt
     4801 tgaatctaat ggagaggaga tcctagctgt tatcagttca atcacttctt ccgcaaaatc
     4861 cataaattcg attggaatct gcgagactat ctcactgaat cgtttgatct tttctttaga
     4921 atctaataca aataatttgt cagcatcctc aacatcaaat ggcttactca ctttagcaga
     4981 aaagccaatc cctttcccca tcgcaatttt ttctattcca tcgtcatcga tcaatacagc
     5041 attatgattc agctttttca tcacatacac cgttaattca cctcctctat atgataaaac
     5101 aaaacaactt gcagactcct tcatacatat ttttgtctcc aaacaatttt taaagacaaa
     5161 aaacgaccta agaaagaacc ctttcttagg tcgtgcctaa ttgaatagta gcaccctaca
     5221 taatttacaa aaatagttta acacataata aaagcgttat caatatcttt tttactcgtt
     5281 ttatgataat acttgtatcg aattgatttg gaggtaatta cataaaaaca aaaaaaatat
     5341 agcaactctc tataagaaag ttgctatatt gataattata ccggcggccg gggtcgaacc
     5401 ggcacgtcct caacggacac tggattttga gtccagcgcg tctgccaatt ccgccacgcc
     5461 ggcaaattta aaaataataa ctggggtagc tggattcgaa ccaacgcatg agggagtcaa
     5521 agtcccttgc cttaccgctt ggctataccc caagaataaa aggcgaatga tgggaatcga
     5581 acccacgaat gccggagcca caatccggtg cgttaaccac ttcgccacat tcgccatcat
     5641 aaaagatgtt atttaatttc tcaataaaaa actaagatga agacatctta gtcaataccg
     5701 gcggccgggg tcgaaccggc acgccctcaa cgggcactgg attttgagtc cagcgcgtct
     5761 gccaattccg ccacgccggc aagttgattt attagtacta aggcggtaac cggatttgaa
     5821 ccggtgatga aggttttgca gacctctgcc ttaccacttg gctataccgc cattatacta
     5881 ttatcaatct ataaaataaa gaataaatta tag
//
LOCUS       DAZKLM010000123         5689 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3764
            SAMEA115706913-rid22582563.denovo.128, whole genome shotgun
            sequence.
ACCESSION   DAZKLM010000123 DAZKLM010000000
VERSION     DAZKLM010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706913
            Sequence Read Archive: ERR13196647
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5689)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5689)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287823.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287823.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,004
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,849
            CDSs (with protein)               :: 2,849
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 41 of 96
            Pseudo Genes (incomplete)         :: 52 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 15 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5689
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706913-rid22582563.denovo.128"
                     /isolate="VRE3764"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(488..1297)
                     /locus_tag="ACG842_002539"
     CDS             complement(488..1297)
                     /locus_tag="ACG842_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFQ0249026.1"
                     /translation="MVKESVIGKKELRSIFWRSFFMQSSWSFDKMMAYGQMNGIERSL
                     RKIYPNDDDYYTALQRHTETFNITPHIAPFVMSLSVTMEEENAKNPEFDTDAINKIKT
                     GLMGPLSGIGDSFFWGTFRVIAAGIGISFAQQGNILGMILYFTLYTAIHFIFKWLATK
                     YGYVLGTKFIDDTEDNHMIDKLSHGASILGLTVIGAMIGTMVTLNTSLKFTVGGAETS
                     LQSIFDQIFPGLLPLGITFLCVWLYSKGHKTITIILGIFVLCFLGTLGGIF"
     gene            complement(1297..2043)
                     /locus_tag="ACG842_002540"
     CDS             complement(1297..2043)
                     /locus_tag="ACG842_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFQ0249027.1"
                     /translation="MIQAILIALVAFLASIDEYTFGASMMGRPLFTAPFVGLILGDLE
                     SGIIIGATLESMFMGSIMVGSATPPEVYASSVLGTAIAIQSGQGVGTAVALTLPLSIF
                     LQMWRNFAYAMPITYFNNKMDEALVNHDFKKAQKYHYVAQPLIVGVPSAILVFVATYF
                     GVSTINAMIKLIPQVVLDSFDVAAGVLSCVGLALLIKMMANKAMMPYLFLGFVGVMYL
                     KMDVVGVAVVGLCLALLAVQKLTFEEEEDL"
     gene            complement(2061..2528)
                     /locus_tag="ACG842_002541"
     CDS             complement(2061..2528)
                     /locus_tag="ACG842_002541"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFQ0249028.1"
                     /translation="MIKLLRVDERFVHGQVAFAWTNNLGANCIFVASNEIATNNLRKK
                     TLKMAVPTGTKFVVKDMTEAISVLNGEKIKKYQVFLIVGTVEDAYEITQHVPEIKEVN
                     LGNLKKNENRKMITSSVYLNEQELDLVKKMTENGITVQCQAVPTDKKINPLHD"
     gene            complement(2528..2938)
                     /locus_tag="ACG842_002542"
     CDS             complement(2528..2938)
                     /locus_tag="ACG842_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFQ0249029.1"
                     /translation="MRKIVIATHSMMAKGLKDTLEFIIGPQKSLLAITAYIDSDYSIN
                     QEIEQLFSDLAPDDELIVTVDLLGGSVSNAFSEQIGKDNFYLISGVNLPLLLELCLSG
                     EQDTKKLIQKAMNSGNEGIVLVNELLEKTEEDDF"
     gene            complement(2949..3962)
                     /locus_tag="ACG842_002543"
     CDS             complement(2949..3962)
                     /locus_tag="ACG842_002543"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFQ0249030.1"
                     /translation="MLGFSEERFLSVLNGAVKNIETINQVADAISEKGFKNLYLVGSG
                     GTYATISPLAYLLSTHSELDYYHEIAAEVVCSQPKKINKDTVLITSSLTGTTQETVDV
                     VKYANSQGATTIAFVGNAATPLGEIVDYAIENDADNDTLVEELQLQFFALGARLMKNN
                     GEFPEYDEFIQTLQKMPEVLLSVRQQNDQKALEFAEKHKNTEFHMFVGAGNTYGAAYE
                     YAMCVLEEMQWIATKSIHAAEFFHGTIEMTDKSMSFVLLKGEDQTRPLVDRVERFVNK
                     YSDVVTVFDTADFELAGVPSAMRQYVAPMVMATALERVSAHFEKVRDHSLDIRRYYRT
                     VEY"
     gene            4179..4913
                     /locus_tag="ACG842_002544"
     CDS             4179..4913
                     /locus_tag="ACG842_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFQ0249031.1"
                     /translation="MNTEELTNKLIQDIEQEKYKYAQAIPSERDFVQIYEMNRSAVKK
                     AIQQLVNKEYLLKIHGKGTFVKKNIHEHLQIKFHGLSELLEREGIQPSSLILASEIKP
                     AGHIVGKALQLEPTEEVFHLVRLRSGDGEPISIEDTYIPLNLIPDLQAFDFQIFSFYS
                     TLAAYHYQIENIYQTISSSKVSNKEARLLKTSLNRPVISLAITAVTTNEQAVEYTEVV
                     VLPEFAAIYTKNVHQGKEMKIYAQID"
     gene            4897..5607
                     /locus_tag="ACG842_002545"
     CDS             4897..5607
                     /locus_tag="ACG842_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFQ0249032.1"
                     /translation="MPKLINLLAYDLIDKIIAYIHEHHLTPGTKLPSERDLSEMFNVN
                     RTTLRGALQKLVDNGTLRAQTNSGYYLTAPKFEKNAHHFFTPIEDSLLSKQRLQLKAT
                     ALSPKKAAALCPELNDFKQQQPQAYLEYIDAEAISLMLLIPMETLKKSNFSDNPFQDF
                     KSLAYTRSQEISLITDNDVAADLLGVDKEEVLLFVREEIRAGKELAAIALGYTISRRC
                     NIQVNVATTLQSGSVAKF"
BASE COUNT         1832 a         1195 c          943 g         1719 t
ORIGIN      
        1 aagcgacaaa aaccacgagc aggtccattc atcctcaaac ctcggtgatc ttcgcggttt
       61 aacaccatca gaagaaatgc acaaaaataa taaggagagt ctgagacata aataaattcc
      121 cagactttta gtgagtctaa atccgaataa acggtgggtc gaaagcaact ctagctgcgc
      181 gtcgatcgca gcaatctcta ggtgctatgt tcggataaat ggtggtcaaa agtcagattt
      241 tcggccattt cgtaacagcc aaccagacaa aaagcgtctg ttttgtcttc tactccaatt
      301 gctcaaatct tagcgacttt tggcttggtc ttactctaaa cagtttgtaa gcctaaactt
      361 tatttgctgt ttgtttctaa gatcaaagca aaactacttt ttagttggtt acttttacaa
      421 gttcttttta ctgttgaata aatgtaaatc ttaagaacaa atatttactt ctataattcc
      481 aagtttctta aaaaattccg ccaagtgtac ctaaaaagca taaaacaaaa attcctagaa
      541 taattgtgat cgttttatgc cctttggagt aaagccagac gcataaaaag gtaattccta
      601 atggcagcaa gcccggaaaa atttgatcaa aaatactttg taatgaagtt tcagctccac
      661 caaccgtgaa ttttagtgac gtatttaaag taaccatcgt gcctatcatc gcgccaatta
      721 ccgttaaacc taaaatcgaa gcaccatgag ataatttgtc aatcatatgg ttgtcttctg
      781 tatcatcaat aaatttagtt cctaaaacat agccatattt tgtcgccaac catttaaaaa
      841 taaaatgaat ggcagtatac aaagtaaaat aaaggatcat gcctaaaata ttcccttgtt
      901 gagcaaaaga aatacctatg ccagccgcaa taacccgaaa agttccccag aaaaatgaat
      961 cgccaatacc tgataaaggt cccattaatc ctgttttgat tttattaatt gcgtctgtat
     1021 caaattcagg atttttagca ttttcttctt ccatggtgac ggacaagctc ataacaaaag
     1081 gcgcaatatg cggtgtaatg ttaaatgttt ccgtatggcg ctgcaacgct gtataataat
     1141 catcatcgtt cggatatatt ttacgtaagg aacgttcaat accattcatt tgtccatatg
     1201 ccatcatttt atcgaaggac caagaggatt gcataaaaaa ggagcgccaa aatatacttc
     1261 ttaattcttt tttcccaata acactttctt ttaccattat aaatcttcct cctcttcaaa
     1321 agttagcttt tgaacagcca gtaacgctaa gcaaagtccg acgacagcca caccgacaac
     1381 atccattttc agatacatta cgccgacaaa tcctaagaaa agataaggca tcatcgcttt
     1441 attcgccatc attttaatta ataacgctag cccaacacaa gaaagcacac cggccgcaac
     1501 atcaaagctg tccaatacaa cctgaggaat caacttgatc atcgcattga ttgtagacac
     1561 accaaaataa gtagctacaa aaactaaaat ggccgatgga accccaacga ttaaaggttg
     1621 agccacataa tgatattttt gtgctttttt gaaatcatga ttgactaatg cctcatccat
     1681 tttgttgtta aaatacgtaa ttggcattgc ataggcgaag ttacgccaca tctgaagaaa
     1741 gatggacaaa ggtaaagtca acgcaacggc agttcctact ccttgacccg attgaatcgc
     1801 aattgctgta cctaaaacac tggaagcata aacttcaggg ggcgtcgcac tccctaccat
     1861 aattgagccc atgaacatgg attccaaggt ggcaccaata ataattcctg actctaaatc
     1921 tcccaaaatc aaaccgacaa aaggcgctgt aaatagcggt ctacccatca tcgatgcacc
     1981 aaatgtgtat tcatcaatag acgccaaaaa agctaccagt gcgattaata ttgcttgtat
     2041 catattcttc ccctcctctt ttagtcatgc aaaggattga tttttttgtc cgttggtaca
     2101 gcctgacatt gaactgttat cccgttttca gtcatttttt tcaccaagtc cagttcttgt
     2161 tcattcaagt aaacggaact agtaatcatt ttccgattct cgtttttctt taaatttcct
     2221 aaattcactt ctttaatctc aggtacgtgc tgagtaattt cgtaggcatc ctccaccgtc
     2281 ccgacaatta aaaatacttg gtatttcttg attttttctc catttaaaac agagatggcc
     2341 tctgtcatat ccttcaccac aaattttgtc cctgtaggaa cagccatttt tagtgttttt
     2401 ttgcgcagat tattggtagc gatttcatta cttgcaacga aaatacaatt tgcacctaag
     2461 ttatttgtcc aggcaaaagc aacttgccca tgaacaaaac gttcatctac tcgtaataat
     2521 ttaatcatta aaaatcatcc tcttctgttt tctctagcaa ctcgttcacc aaaactatcc
     2581 cttcattccc gctattcata gctttttgga ttagtttttt cgtgtcctgt tcacctgata
     2641 agcaaagctc taaaagcagc ggcagattta caccgctgat taaataaaaa ttgtctttac
     2701 cgatttgttc ggagaaagcg ttagacacgc ttcctcccag taaatctacc gtcacaatta
     2761 attcgtcatc aggcgctaag tcggaaaata gctgctctat ttcctggtta atggagtagt
     2821 cactatctat atacgcagtt attgctaaaa gtgatttttg aggaccaata ataaattcta
     2881 aagtatcctt taagccttta gccatcatgg aatgcgtcgc aattacaatt ttcctcatag
     2941 ctcttacctt aatattccac agtccgataa taacgacgaa tatctagtga atgatcccgc
     3001 accttttcaa aatgagcact cactcgttct aaggccgttg ccataaccat tggcgctaca
     3061 tactgacgca tagcgcttgg tacacctgct aattcgaaat ccgcagtgtc aaaaaccgtc
     3121 acaacatcgc tatatttatt tacaaaacgc tctacgcgat cgactagcgg acgcgtttga
     3181 tcttcacctt ttaataacac aaaactcata gatttatcag tcatttcaat agtcccatgg
     3241 aaaaattcgg ctgcatggat cgacttggtg gcaatccatt gcatttcttc cagtacgcac
     3301 atagcatatt cataggcagc cccataagta ttcccggctc caacaaacat atgaaattcc
     3361 gtatttttat gtttttctgc aaattcaagt gctttctgat cattttgttg ccgaacactt
     3421 agtaatactt ccggcatttt ttgtaatgtt tgaataaatt catcatattc aggaaactca
     3481 ccgttatttt tcatcaggcg tgcacccaaa gcaaaaaact gtaactgaag ttcttctact
     3541 aatgtatcgt tgtcagcatc attttcaatc gcataatcca cgatttcccc gagtggtgta
     3601 gcagcatttc ctacaaacgc aatcgtcgtt gcaccttgac tattggcata cttaacaaca
     3661 tcaacggttt cttgagtcgt accggttaat gaagatgtga ttaacactgt atctttatta
     3721 atctttttcg gctgtgaaca aaccacctct gcggctattt catgataata atcaagctct
     3781 gaatgagtgc ttaacagata agctagcggg ctgattgtgg catatgttcc accagagcct
     3841 accaaataaa gatttttaaa tcccttctca ctgattgcat ctgccacctg attgattgtc
     3901 tcgatatttt ttactgcacc atttaatact gatagaaaac gttcttcgct aaaacctaac
     3961 atagttaaaa acctccaagt caataatttg aaaaatatat ttgcaaatgc cggcacatta
     4021 acaaaccact taaccttttt tatataccac ctgttaacgt ttttaatgta ccacttatag
     4081 aaaacgctgt caatagtttt taaccacttt tatttttatt tgtaccaaaa tccttttttc
     4141 atgtataata aacaaaaata gactctggag tgtaacgtat gaatactgaa gaactaacga
     4201 acaaattaat acaagacatt gaacaagaaa aatataaata cgcgcaagct attccttcag
     4261 aacgtgattt tgttcaaatt tatgaaatga atcgttcagc cgtaaaaaaa gccattcaac
     4321 aattagtaaa taaagagtac ctactaaaaa tacatggcaa aggaactttt gttaaaaaaa
     4381 atatccatga acatttgcaa atcaaatttc atggtttgtc tgaattgttg gaacgggaag
     4441 ggattcagcc tagcagctta attttagctt cagaaataaa accggctgga cacattgtcg
     4501 ggaaagcttt acagctggaa ccaactgaag aagtatttca tttggttcgt ttaagaagcg
     4561 gcgacggtga gccaatttct attgaagaca cttatattcc attaaatctc atacctgatc
     4621 ttcaagcttt tgatttccaa attttttcat tttattccac attggctgct tatcattatc
     4681 aaattgaaaa tatctatcag acaatttcca gctcaaaagt gagcaataaa gaagcccgtt
     4741 tgttaaaaac cagcttaaat cgtccggtaa tcagccttgc tatcactgcg gttacgacaa
     4801 atgaacaagc ggttgaatac actgaagtag tggttttgcc tgaattcgcc gccatctata
     4861 ctaaaaatgt tcatcaagga aaggagatga aaatctatgc ccaaattgat taatctgttg
     4921 gcttatgacc tgattgacaa aattattgct tatattcatg aacatcattt aacccccgga
     4981 acaaaactgc cttcggaacg ggacctatca gaaatgttta atgttaatcg aactacctta
     5041 agaggcgcgt tacaaaaact ggtggacaac ggcaccttac gggcgcaaac caatagcggc
     5101 tattatttaa ctgcgccaaa attcgaaaaa aatgcccatc atttctttac accgatagaa
     5161 gattctctgc ttagtaagca gcggctacag ctaaaggcaa cggctctttc accgaaaaaa
     5221 gcagcagctc tttgtccgga actgaatgat ttcaaacagc agcaaccaca agcttattta
     5281 gaatatattg atgcagaagc aattagcttg atgttattaa tccctatgga aacattaaag
     5341 aaaagtaatt tctcggacaa tcctttccaa gattttaaaa gtttggcata tacgcgttct
     5401 caagaaattt ctttaataac tgacaatgac gttgcggcgg acttgctggg agttgacaaa
     5461 gaagaggttc tgttatttgt acgagaggaa ataagagccg gtaaggagtt ggcggcgatt
     5521 gctttaggtt atactattag tcgccgctgc aacatacagg ttaatgttgc tacgaccttg
     5581 caatccggtt ctgttgcaaa gttttaaatc tactatcaga taaggtagaa taatagaaaa
     5641 agatagcagg aggaatgacg atgagtcatt ttaaaggaaa gcaatttca
//
LOCUS       DAZKLM010000124         5481 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3764
            SAMEA115706913-rid22582563.denovo.129, whole genome shotgun
            sequence.
ACCESSION   DAZKLM010000124 DAZKLM010000000
VERSION     DAZKLM010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706913
            Sequence Read Archive: ERR13196647
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5481)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5481)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287823.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287823.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,004
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,849
            CDSs (with protein)               :: 2,849
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 41 of 96
            Pseudo Genes (incomplete)         :: 52 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 15 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5481
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706913-rid22582563.denovo.129"
                     /isolate="VRE3764"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(43..409)
                     /gene="ssrA"
                     /locus_tag="ACG842_002546"
     tmRNA           complement(43..409)
                     /gene="ssrA"
                     /locus_tag="ACG842_002546"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(580..1353)
                     /locus_tag="ACG842_002547"
     CDS             complement(580..1353)
                     /locus_tag="ACG842_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFQ0249033.1"
                     /translation="MSNYRAITFFDLDGTLLDAKSKITPEVAKAMNALKENNVLPVIA
                     TGRTEAEIHSIMEDAGITSAVTMNGSFIRVDGEEIYSQTFSSEECQKMYDHVQLNGHE
                     LSYYNDQKIWCTGHSQTMINAYDFIHSDVPEIDPLGFKENTVNMLLILSQSGDEYYHE
                     HFPELTFYRNGPYSIDTVKKGISKGAGVKRLQKELQLEKVPTFGFGDGPNDFALLEAC
                     DNKIAMGNAYDGLKELSTFITKKNTEGGIVHALKYFDLI"
     gene            1497..1847
                     /locus_tag="ACG842_002548"
     CDS             1497..1847
                     /locus_tag="ACG842_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HFQ0249034.1"
                     /translation="MIFFSIGILFLIIGSLFRILPSKGNLPFYGYHSPLAAKTDAHWR
                     LAQKTSGNWFFLMGLLMALIGYYLKTSGHTNYFLIEMLLLVFPIMPIFIMTEKKLQKY
                     DLETGGNDNEYFND"
     gene            1828..2544
                     /locus_tag="ACG842_002549"
     CDS             1828..2544
                     /locus_tag="ACG842_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HFQ0249035.1"
                     /translation="MNILMIEDNQSVSEMMQMFFLNEGWEATFKYDGKEGLDAFLENP
                     QHWDMITLDLNLPTLDGMLVGREIRKVSSTVPIIMLTARDSESDQVIGLEMGADDYVT
                     KPFSPLTLIARMKALHRRSELAEAREEAVVSDDHFDIETSHFKMNTKTREAYLDNRPI
                     EGLTPKEFDLLFTLAKKPRQVFSREQLLELVWDYQYFGDERTVDAHIKKLRQKIEKVG
                     PQVIQTVWGVGYKFDDSGVA"
     gene            2544..3992
                     /locus_tag="ACG842_002550"
     CDS             2544..3992
                     /locus_tag="ACG842_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFQ0249036.1"
                     /translation="MRYLYQQLLAFCGMVALILLIVGVSFTQLTKQTIEENNYQQLFG
                     YAESVEKTTQTYADTLPQFSQDQAFQNALLLTEQVLTEQNVNFIFIDKNERVIYPTNT
                     ALLNFTLTADQWKNLRNGRQEKFTSNKNILGQAQATSYALVPFNLNHEFYGALVVSQP
                     ARNIDNSVRPITLNLFKGFIFSSIVAVIASYAFAAFQVKRINRLRNATKEVTNGNFDV
                     QLPVHDKDEFDELADDFNKMTNSLKESQAEIEEQENRRRQFMADASHEMRTPLTTING
                     LLEGLEYNAIPENQRENAIKLMKNETERLIRLVNENLDYEKIRTNQISMVIKKFNGTE
                     TLKNIVAQLEAKAEAAGDTLTLKAVDDIDVYADYDRFVQIMVNIIQNAIQFTENGQIT
                     ITLEKGYLETIITIEDTGIGMSEQQMKSIWDRYYKVDPSRKNTKYGESGLGLPIVQQL
                     VRLHKGKLEVESELGKGTKFAVRLPDVEIKKE"
     gene            complement(4062..4571)
                     /locus_tag="ACG842_002551"
     CDS             complement(4062..4571)
                     /locus_tag="ACG842_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0249037.1"
                     /translation="MKPEYLIHAEHLTVILCPLEKQHKILEHLKKEVLVAKLDNFAII
                     QKNWPMFPYLNLKDHVLLDVPEKEIKQDRLAYQEKLDISPSLLNCAAAELTSFEKVKL
                     QLLHALLSKRNNIVIEDTFDELSVLEIQELLHLLSYLAHEENQGILLFTHDATIAQSP
                     YIDRLEPAG"
     gene            complement(4561..5286)
                     /locus_tag="ACG842_002552"
     CDS             complement(4561..5286)
                     /locus_tag="ACG842_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0249038.1"
                     /translation="MKIIRYATARLIYYKKQTILYCLFSSFSAFLLIACLTLYHLQSA
                     LANQVQDRLSFIGASASTELLPALELASRFYIKGIFSLFFLFAFLFIFFFYYSVRQNK
                     QELINWHLTGLSKTKLFLFIFWQLFFPLFLCCILVLLWIVSFQSVYEALLQRINFLVL
                     KTTELPDIRDILTSVSGLTIPMDQQTFFTIDFQNDLFFTDILKGFAQTLIGLSGCCLI
                     LSLFPFTFFIHHLQSRRPGRNET"
BASE COUNT         1864 a          955 c         1095 g         1567 t
ORIGIN      
        1 aaataaggat ttaagcatct agcgaaatct gctgaaatct cacaatggag acggcgggag
       61 tcgaacccgc gtccaaacac atcgccactt aaaaatctac gttcatagtc aactcattta
      121 aggattcact ttacagcttg ccgagtgaca ggcattctgt atcgctagtc tgatgttctc
      181 tttttcaact tacagacgga aagttgaaac gtatcccact aaagttagga cccttactcg
      241 agcacatggg cgatgccgag aggatctacg ctaactgttt ttaggcagct aaagcgtaag
      301 agttgttttc gtttttagca gttatattta actgtaacgt ttttacgtag acgtaaccta
      361 cgaaacgcaa ttcaagctcg actgtgcctg tcgaatccgt aacgtccccg ttagctaaca
      421 agggttacgt gtatttttca tacaaaatta gtatagcata tattgggata aattcaataa
      481 aaaagcgaat catatcccat tatttatata ataactgccc ctttcttaag aaaaaataag
      541 cgattttctt aagaaaagag gtagcttcaa aaaaagatct taaatcagat caaaatactt
      601 caatgcgtgg acaattccgc cttctgtatt tttctttgtg ataaaggtag aaagttcttt
      661 taatccgtca taggcgttgc ccatagcgat tttgttgtcg caagcttcca acaaggcaaa
      721 atcattcgga ccatcgccaa atccaaaagt aggaactttt tccagctgaa gttctttttg
      781 cagacgtttg acacctgccc cttttgagat tcctttcttc actgtatcga tcgagtaagg
      841 accattccga taaaaagtta gctcaggaaa atgttcgtga tagtattcgt cacctgattg
      901 agataagatc agcaacatat ttactgtgtt ttctttaaag cctaaaggat caatttccgg
      961 aacatctgaa tgaatgaagt cgtaagcatt gatcattgtt tggctatgac cggtacacca
     1021 gattttctga tcgttatagt aagaaagttc atgaccgttc aactgtacat gatcatacat
     1081 tttttgacat tcttctgaag aaaatgtttg agaatagatt tcttcgccgt ctactcgaat
     1141 gaaagaacca ttcatggtga ccgcagaagt aatgcccgca tcttccatga tagagtggat
     1201 ttctgcttct gtacgtcctg tagcgatgac ggggagaaca ttgttttctt ttaatgcgtt
     1261 catagctttt gccacttcgg gtgtgatttt tgatttcgca tctagtaatg ttccatcaag
     1321 atcaaagaaa gtgattgcac gataatttga cacaaataaa actccttttt tattggaata
     1381 agtttatctt aacaaagaat gaacaaaaga caaacctttc gtgaagaatt cctgaacata
     1441 ttggttcagt caacaattta gagtatacta taagaagaga atttaggagg gaaagaatga
     1501 tttttttcag tattggtatc ttattcttaa taattggcag tctttttcgg attctgccct
     1561 caaaaggaaa tctgcctttt tatgggtatc attcgcctct tgccgcaaaa acagacgctc
     1621 attggcggct ggctcaaaag acaagcggaa actggttttt tcttatgggc ttactgatgg
     1681 cattgatcgg ctattattta aagacatctg ggcatacgaa ttattttctg attgaaatgc
     1741 ttctgctagt atttccaatt atgcctattt ttattatgac agaaaagaaa ttacagaaat
     1801 atgacctgga aactggaggg aacgataatg aatattttaa tgattgaaga taatcaatct
     1861 gtttctgaaa tgatgcagat gtttttctta aatgaaggtt gggaagcaac tttcaagtat
     1921 gatggaaaag aagggctcga tgccttttta gaaaacccac aacattggga tatgattaca
     1981 ctagacctga atttgcccac actggacggt atgctagtag gaagagagat ccgcaaagtt
     2041 tcaagtactg tgccgattat catgctgaca gctagagatt ctgaaagcga tcaggtgatc
     2101 ggcttagaga tgggggcaga tgattatgtc acaaaacctt tcagtcctct gacgcttatt
     2161 gctcgaatga aagctttgca tcgccgtagt gagttggcag aagcaagaga agaagcagta
     2221 gtttcagatg atcattttga tatcgagact agtcatttca agatgaatac gaaaacacga
     2281 gaagcgtatc tggacaaccg gccaattgaa ggactgacgc ctaaagagtt cgatctattg
     2341 tttactttag caaaaaaacc acgtcaagta ttctctcggg agcagttgct tgaattagtc
     2401 tgggattatc agtactttgg tgatgaacgg acagtggacg cccatatcaa gaaattacga
     2461 caaaaaatcg aaaaggtcgg accacaagtc atccaaactg tttggggagt cggctacaaa
     2521 ttcgatgatt ctggtgttgc ctaatgcgtt atttgtatca gcaactctta gctttctgcg
     2581 gaatggtcgc gttgatctta ttgatcgttg gagtttcttt tacacaactg acaaaacaaa
     2641 cgattgaaga aaacaattac cagcaactgt ttggatatgc tgaatcagtt gaaaagacaa
     2701 cgcaaaccta tgccgatacg ctcccgcagt ttagtcaaga tcaggcgttt caaaatgcat
     2761 tgctgctgac agagcaagta ctgacagaac aaaatgtgaa ctttatattt atcgataaaa
     2821 acgaacgtgt catttatccg actaatacag ctttactgaa ttttacctta acagctgatc
     2881 aatggaaaaa tctgaggaat ggacgtcaag aaaaattcac atcgaataaa aatattttag
     2941 gacaagcaca ggcgacctct tacgcacttg tgccatttaa tttgaatcat gaattctatg
     3001 gagcacttgt agttagtcaa cctgcgcgaa atatcgataa cagcgttcgt ccaattacac
     3061 tgaacctgtt caaaggattt attttttcca gtatcgttgc agttattgcc agctatgctt
     3121 ttgcagcatt ccaagttaag cgtatcaatc ggttaaggaa tgcgacgaaa gaagtgacga
     3181 atggtaattt tgatgtacag ctccccgtac atgacaaaga tgaatttgat gagttagcag
     3241 atgatttcaa taaaatgacg aattcattga aagaatctca agcagaaatt gaagaacaag
     3301 aaaatcgtcg aagacaattt atggcagatg cttctcacga gatgagaaca cctcttacga
     3361 caatcaacgg acttctggaa gggttagaat acaatgcaat ccctgaaaat cagcgagaaa
     3421 acgcgattaa attgatgaaa aatgaaacag agcgtctgat ccgtttggtg aatgaaaact
     3481 tggattatga aaaaatccgt acgaatcaaa tcagtatggt cattaaaaaa ttcaatggaa
     3541 cagaaacatt gaaaaatatt gttgcacagt tagaagctaa agcagaagca gcaggagata
     3601 cattgacatt gaaagccgtt gatgatattg atgtttacgc tgattatgac cgatttgtcc
     3661 aaatcatggt caatattatt caaaacgcga tacagtttac tgaaaacggc caaatcacga
     3721 ttactttaga aaaaggctat cttgaaacga tcatcacgat tgaagatacg ggaattggaa
     3781 tgtctgaaca acaaatgaaa agtatctggg atcgttatta taaagtagat ccttcaagaa
     3841 aaaatacaaa atatggtgaa tcaggattag gtttaccaat cgtgcagcaa cttgtacgct
     3901 tgcataaagg aaaattagaa gtagaaagcg aactaggaaa aggaacaaaa ttcgctgttc
     3961 gtttgcctga cgtagaaatc aaaaaagagt aaaaaacaag cggtataaaa gatcatgaat
     4021 cgatcttcta taccgcttgt ttttactata aaatgattga tttagcccgc gggctctaaa
     4081 cgatcaatat aaggactttg agcaatagta gcatcatgag taaagagtaa gataccttga
     4141 ttttcttcat gagctaaata agaaagcaag tggagtagct cttggatctc caatacacta
     4201 agttcatcaa atgtatcttc aataacaata ttgtttcgct tggaaagcaa ggcatgaagt
     4261 aattgcagct ttactttttc aaaagacgtc aactcagctg ccgcacaatt aagcaaggaa
     4321 ggagagatat caagtttttc ttgataggcc aaacgatctt gtttgatttc tttttccggt
     4381 acatccaaca ggacatggtc tttaagattc agataaggaa acatcggcca atttttttgg
     4441 atgatcgcaa aattatccag ttttgcaaca agaacctctt ttttcaagtg ctccaatatt
     4501 ttatgctgtt tttcgagagg acacaaaata acggtcaagt gttcagcatg aatcaaatat
     4561 tcaggtttca ttacgtccag gcctcctact ttgcaaatga tggataaaaa aagtgaaagg
     4621 gaatagacta agaatgagac agcatccact gagcccaata agagtttggg caaatccctt
     4681 aagtatatct gtgaaaaaaa gatcattttg gaaatcgatg gtaaaaaaag tctgttgatc
     4741 catcggaatt gtaagtccgc ttaccgaagt caagatatca cggatatctg gcagttcagt
     4801 agtctttaag acaagaaaat tgatcctttg aagaagcgct tcataaactg actggaagct
     4861 tacgatccac aataaaacca agatacaaca aagaaagaga ggaaagaaca gctgccagaa
     4921 aataaaaagg aataatttgg ttttagaaag accagtcagg tgccaattga tcaattcctg
     4981 cttgttttgc ctgactgaat agtaaaagaa aaagataaaa aggaaggcaa atagaaaaaa
     5041 taaactgaat atgcctttta tataaaatcg gctagctaat tctaaagccg gaagaagctc
     5101 tgtagatgca gaagcgccaa taaatgaaag ccgatcttgg acttgattgg ctaaagccga
     5161 ttgcaaatgg taaagagtca ggcatgctat caataaaaaa gcagaaaagc ttgaaaataa
     5221 acaatagagt attgtctgct ttttgtaata aattaatcga gcagtagcat agcggattat
     5281 tttcatactt ttcacctcta taaaaagtat agaaaatgaa tatgaataaa gtatgaaatt
     5341 cctttagtga aatcacgaat taaaaccaga aaatattttg taaaaagcaa gaggttgtga
     5401 agaagctgcc ctattctatt accgtagatg caagcttttg aacactgttt attcgaaaag
     5461 aggagcagcg acaataattt t
//
LOCUS       DAZKLM010000125         5318 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3764
            SAMEA115706913-rid22582563.denovo.130, whole genome shotgun
            sequence.
ACCESSION   DAZKLM010000125 DAZKLM010000000
VERSION     DAZKLM010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706913
            Sequence Read Archive: ERR13196647
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5318)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5318)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287823.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287823.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,004
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,849
            CDSs (with protein)               :: 2,849
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 41 of 96
            Pseudo Genes (incomplete)         :: 52 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 15 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5318
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706913-rid22582563.denovo.130"
                     /isolate="VRE3764"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(538..972)
                     /locus_tag="ACG842_002553"
     CDS             complement(538..972)
                     /locus_tag="ACG842_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0249039.1"
                     /translation="MRLYHLRSLEELRKLDFNKKFFWEISDLSCFVESERLSNGNNSV
                     LNEYDKVVINKVKSLCEEARESISHINGSNFKQTLFNLIKLDAKISSYLFFLIHDEFM
                     DYQKLDQLIERESWEDYYVGLTFSEKLNNYKLIDYKYLSESN"
     gene            complement(989..2194)
                     /locus_tag="ACG842_002554"
     CDS             complement(989..2194)
                     /locus_tag="ACG842_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-targeted surface antigen transcriptional
                     regulator"
                     /protein_id="HFQ0249040.1"
                     /translation="MFSDKEVLLKSLHVCLKLPIVVFNSEFSIIKEYRSDRTTYFFYD
                     YKKLLTDNIKKTKDFHFFTGDFNEMFLLYMFKTRYYLFGPFRANNIDKVFFKLKMNNL
                     NVAMADRERLYNSLQNLTLYSLGDIRDILILVHYFFTGKIEDLFHEPLIEYTENLSKT
                     IEQIKIDNLLSQNYDPEIYLFLYENKILEYVKNGDIRNLENMVFNLSNGIIPSVSGDT
                     IRSEKNYSIIVFEKLAQTSITLGMDIIEAYQSRDALIQENELAVSLPEVLKVRDSGIV
                     YYTKEIGKTKIEHLSPLISSVVQFIGLNIYKRITVKEIANYFSVSETKLRESFKNEMH
                     ITIYNYISKRKISTAKIMLKSNHTISEVSLGLGFSDSSHFSRVFKKYAGVSPKQYQLG
                     LVDNFNNIS"
     gene            2888..3274
                     /locus_tag="ACG842_002555"
     CDS             2888..3274
                     /locus_tag="ACG842_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HFQ0249041.1"
                     /translation="MSDNDERVNAQLIRFFEKVILNTANTYYKKQEEIQKHEQLDQLP
                     PYLFTKDTIKFKQPINVLNVTLLVEDIKLAKILPLLKEPEQIFLIEKFIFDKTDKEIG
                     KVLGITRQGATNLKHRLYKKIQKMLV"
     gene            3368..3559
                     /locus_tag="ACG842_002556"
     CDS             3368..3559
                     /locus_tag="ACG842_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0249042.1"
                     /translation="MNSLKEDFILAKAGNEEAVEAILKRFSSLIHKQSWRTGKYDQDC
                     YQECMLAIYLAISKFEIKE"
     gene            4073..4405
                     /locus_tag="ACG842_002557"
     CDS             4073..4405
                     /locus_tag="ACG842_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFQ0249043.1"
                     /translation="MRKTYDRKFKLKISQDILEKKVTTKKIAEEYNISRPTISRWVSE
                     YRRYGKNAFAGQGKRLPDKADFYILEQENKRLKEEDECSTNTKIDTDLQGSLKNFTMK
                     ESKSINVL"
     gene            4315..4509
                     /locus_tag="ACG842_002558"
     CDS             4315..4509
                     /locus_tag="ACG842_002558"
                     /inference="COORDINATES: protein motif:HMM:NF024673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFQ0249044.1"
                     /translation="MFYEHKDRYGSPGITQKLYDEGIEINKRVVAVLMREMNLCTRGF
                     HRRKSSHGREKTIKEMVKKC"
     gene            4548..4694
                     /locus_tag="ACG842_002559"
     CDS             4548..4694
                     /locus_tag="ACG842_002559"
                     /inference="COORDINATES: protein motif:HMM:NF012870.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0249045.1"
                     /translation="MMDITYIPCNDGRLYLSTYIDLATRILRYYMVDSHMKKEIVVRP
                     LQIY"
     gene            4701..4856
                     /locus_tag="ACG842_002560"
     CDS             4701..4856
                     /locus_tag="ACG842_002560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HFQ0249046.1"
                     /translation="MPTVIHSDRGSQHRSFIYQELLNDHHITHSVSQTGTPVDNAVIE
                     AFHRSIK"
     gene            5132..>5318
                     /locus_tag="ACG842_002561"
     CDS             5132..>5318
                     /locus_tag="ACG842_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HFQ0249047.1"
                     /translation="MSYSEFTKELLDILDLNLIFHEDAFRKERINDETCFVFDGTLTY
                     QPEECFHRHYQNEQTIIK"
BASE COUNT         1896 a          877 c          744 g         1801 t
ORIGIN      
        1 gaatcttact aaccttatgt taactaattt acttagagaa tttcatgaac attacaacaa
       61 aaaaagtaca tttcttcatg tttttttcgg atatttgtgc tttttaacca ttcagcagct
      121 gaaaacatat aaaaagatat gtatgtattc tttctgaaat taagttcatt tctatgcgat
      181 gaagcatgtt ttcaaaatat taccctttct tagattaaac ttcagagtat tcaaataacg
      241 gaataaggag ccttatcccg cattactgaa tgagactgtg acataagtaa aaaggtgact
      301 tcaaattcct tcttcggaat ttgaaatcac cttctttata attataaagt atcggtttca
      361 cagcatttct gaatgattcc tagaatctta aacgaatgat tggagttatc aacatttttt
      421 cactcgtttt tagcgaaaca aaaaatgaca tttgtcacag cctcattaaa ttattgattc
      481 tttttcttta taattgaatt gtccatattt aaagtgtcta gtactctaca tgaataatta
      541 attgctttca ctcaaatact tataatctat taacttgtag ttgtttaact tttctgaaaa
      601 agttagaccc acgtaataat cttcccagct ttctctttca attaactgat ccaatttttg
      661 ataatccata aattcatcat gtatcaaaaa gaataaataa cttgatattt tagcatctag
      721 cttaatcaga ttaaataatg tttgtttaaa attgctacca ttaatatgac tgatagattc
      781 tctagcttct tcacataaac tttttacttt attaattact actttgtcat attcatttaa
      841 cacactatta ttaccattac ttagacgctc agattctacg aaacaactca aatcagaaat
      901 ttcccaaaaa aattttttgt taaaatccaa tttcctcaac tcctccaaac tacgtaaatg
      961 atatagtctc ataataaaat aaaacctctc agctaatgtt gttgaaattg tccactaaac
     1021 ctaattgata ttgcttagga gatacacctg cgtacttttt gaatactctc gaaaagtgtg
     1081 aagaatctga aaatcctaaa cctaaagaaa cttcactgat agtatggttt gatttcaaca
     1141 ttattttagc cgtagaaatt tttcgcttag agatataatt ataaatcgta atatgcattt
     1201 cattcttaaa gctttctctt aatttagttt cacttacaga aaaataatta gctatttcct
     1261 ttacagtaat tcttttatag atatttaagc cgatgaattg aacgacagaa gaaattaagg
     1321 gagaaagatg ctctatctta gtttttccga tttccttagt ataataaaca atcccagaat
     1381 ccctcacttt taagacctca ggtaacgata cagctaattc gttttcttgt atcaaagcat
     1441 ctctactttg atacgcttct attatatcca ttcctaatgt tatagacgtt tgtgctaact
     1501 tttcaaaaac aatgatagaa taattttttt cagaccgaat cgtatctcca ctaacacttg
     1561 gaattattcc attactcaga ttaaaaacca tgttttctaa atttcgaata tctccatttt
     1621 taacatattc cagtatttta ttttcataaa gaaacagata tatttctgga tcgtaatttt
     1681 gtgataacag attatctatt ttgatttgct cgatagtttt acttagattt tctgtatatt
     1741 caattaacgg ttcatggaat agatcttcta tttttccagt aaaaaaataa tgaactaaaa
     1801 tcagaatatc tcgaatgtct cctaaagaat acaaagtcaa attttgcaat gaattataga
     1861 gtcgttctct atcagccatt gcaacattca aattgttcat ttttagttta aaaaaaactt
     1921 tatcaatatt atttgctcta aatggaccaa ataagtaata tctggtttta aacatgtata
     1981 gaagaaacat ctcattaaaa tcaccagtaa agaaatggaa atctttagtt tttttaatat
     2041 tatcagttag taacttttta taatcataaa aaaaataagt agttctatct gagcgatact
     2101 cttttataat actgaattcg gaattaaaaa ccacgatagg taactttaaa caaacatgaa
     2161 gagactttaa taagacttct ttatctgaaa acacttttgt ttctctcttt cctttctaca
     2221 tcattaaata gtattttata cgttatctaa aatactattc ctctgtcaaa ataagctaac
     2281 ttacttcatt ataaaatatc cacttatcgc tttcatgtat attccaccgt aaaaagtaca
     2341 ttttatcacc gagaactcat catttttcat catgatgaat actatttaca tatatctagt
     2401 tcaaagacaa taattattat gatccgttat tatctaagca ctatacagat aataggaatt
     2461 tttcgtttct aaatattcca atttgggaat ccaataaaaa ttttcgttca aatacgtgtc
     2521 tattcgaact cttttctagt tgttaattat gaagggggaa gaaataaaaa atacaatcta
     2581 tacacgaata agaatattat ccaaaaggag gttttagaaa agatttatta tacgtaaaaa
     2641 gctcaagaca ttttcttagc cataaggaca tgtttgatat ttaagaagat atatgaattt
     2701 tcttttctaa gtataaagat aactatccat tatcaactaa aaaaacattt ttgaaaataa
     2761 acatcagcag tcctatcaat gcatgtgagc ttatagaagt tattcgcagc gcagcataat
     2821 tgcattagct tcttttcgtg ttttttgcct cttttccctt taaatcttaa tggaggaggt
     2881 gtgcaatatg tcagataacg acgaacgcgt aaatgcccag ttgattcgat tctttgaaaa
     2941 ggtcattttg aacaccgcta atacttatta taaaaaacaa gaagaaattc aaaaacatga
     3001 acaacttgat caactcccac catatttgtt tactaaagat actataaaat ttaaacaacc
     3061 gattaatgtt ttaaatgtga ctctccttgt tgaagatata aaattagcaa aaatccttcc
     3121 tttgttgaaa gaacctgaac aaatttttct tatcgaaaaa tttatctttg ataaaactga
     3181 taaagaaatt ggaaaggttc tggggattac aagacagggt gcgacaaatt taaaacatcg
     3241 actttacaaa aaaattcaaa aaatgctggt ttaatatctg cataataact aattttgtat
     3301 gcttctttgc tcgttctcct ccttcccaaa gaaatagatt ataagtccat gggaagaagg
     3361 tagatttatg aattctttaa aagaagattt cattttagca aaggctggaa atgaagaagc
     3421 tgttgaggca attctcaaaa gatttagctc tttgattcat aagcaatcct ggagaactgg
     3481 aaaatatgat caagattgtt atcaagaatg tatgcttgca atatatctag ctatctcgaa
     3541 attcgaaatt aaagaataac tcaactaagg gagtctgtga cataagtaaa aagatagttt
     3601 caaatttcga acaataaatt tgaaactatc ttctttataa ttataaagta tcgattttac
     3661 agcgttctat aatgattcat agaatctaaa acgaatgatt ttaattatca acgtttttta
     3721 attcgttttt agcttaaaaa taacttttgt cacagtctca ctcctaaaat tactttattt
     3781 acgtatgaag tgggcaggag gtcgaatcca gaactgggct atggtacttc aacgaggact
     3841 tctcctctag aattgaaaag catgcgattt accttccgta acgacggtag atttattcaa
     3901 ctatgtgtcc aatcaaaatt tgtctgattt caaaaatagt caagagaatt ttggttgtta
     3961 aaaactctta tctatcaact tccacagttt agttgacaaa ccctgatact aagttgggct
     4021 atgatacact tttcttgact atcagttacg ttaccggaag gaatgaactc tattgagaaa
     4081 aacatatgat cgtaaattta aactaaagat ttcacaggat atccttgaga aaaaagtaac
     4141 taccaagaaa attgctgaag aatacaatat ctctcgtcca acaatttccc gatgggtttc
     4201 agaatatcgt cgatacggga aaaatgcttt tgctggacaa ggaaaaagat tacctgataa
     4261 agcagatttc tacattcttg aacaggaaaa taaacgacta aaagaggaag acgagtgttc
     4321 tacgaacaca aagatcgata cggatctcca gggatcactc aaaaacttta cgatgaagga
     4381 atcgaaatca ataaacgtgt tgtagctgtt cttatgcgtg aaatgaattt atgcacaaga
     4441 ggatttcatc gacgcaaatc ttctcatggt agagaaaaaa caatcaaaga aatggtaaaa
     4501 aaatgttgaa tcgataattt gaacagaacc aaattgatgc tgtttgggtg atggatatta
     4561 cttacattcc atgtaatgac ggtcgcctct atttgtccac ctatattgac ttagcaactc
     4621 gtattctacg ttactatatg gtagattcac acatgaaaaa agagattgtc gtccgtccat
     4681 tacaaattta ttaaggagaa ttgcctacgg taattcattc cgatagaggt agccagcatc
     4741 ggtcttttat ttatcaagag ctgttgaacg accaccacat cacacattca gtgagccaaa
     4801 ctgggactcc tgttgacaat gcagtaattg aagcgtttca tcgatcaatc aaatgagaat
     4861 taatcgactc caacaaatat aaaagtaaaa tagagatgaa ggtgttaatc caagattatt
     4921 tagaagacta ctacccaaac aaaagaatcc atacaaaatt tatgatgact ccttgacaat
     4981 ttgaagcgaa gaaaataagg gatctataat atctagtgtt ggtgacgcct tctgttgcca
     5041 atactttttc ttttttctaa taaacgcaaa cagacacctt aaaatccttt agaataaagt
     5101 cgacgaaaac cattcaaagg agtgtttcaa gatgtcctat tcagagttta ccaaagagtt
     5161 actagatatt ctagacctaa atcttatctt tcatgaagat gcctttcgca aagagcgaat
     5221 caatgacgaa acatgttttg tttttgatgg cacattgacc tatcaaccgg aggaatgctt
     5281 tcatcgccac tatcaaaatg aacaaacgat cattaaat
//
LOCUS       DAZKLM010000126         5312 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3764
            SAMEA115706913-rid22582563.denovo.131, whole genome shotgun
            sequence.
ACCESSION   DAZKLM010000126 DAZKLM010000000
VERSION     DAZKLM010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706913
            Sequence Read Archive: ERR13196647
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5312)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5312)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287823.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287823.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,004
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,849
            CDSs (with protein)               :: 2,849
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 41 of 96
            Pseudo Genes (incomplete)         :: 52 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 15 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5312
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706913-rid22582563.denovo.131"
                     /isolate="VRE3764"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(74..1222)
                     /gene="nagA"
                     /locus_tag="ACG842_002562"
     CDS             complement(74..1222)
                     /gene="nagA"
                     /locus_tag="ACG842_002562"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HFQ0249048.1"
                     /translation="MRTFIYADKFFLASGVKHAGYLEITDGLFGEYTKELPEGEVNII
                     DQSGKWIAPGLVDTHIHGYMNHDVMDNDAEGIKVMSEGLLSCGVTSFLPTTLTSSKER
                     LKDVAETIGKMYQEAPGAKIKGIYFEGPFFTEEHKGAQNPSYFGDPDLDTFHEWQEAS
                     GGLIKKIALAPERQGVEEFVKQVTEEGVVVSLGHSNATLEEAQKAVEAGASVFVHAFN
                     GMRGLNHREPGMVGALLSLEHVFSELICDGHHVHPNAAGILMEKAGHDHVALITDCMM
                     AGGMPDGNYNLGEFPVVVKEGTARLESGNLAGSILKLKEAIKNVVDWGLATPEQAVMM
                     ASLIPAISCKIDDTCGKIAQGRDADFIVLNPDMTLEATYLDGVERFHA"
     gene            complement(1298..2134)
                     /locus_tag="ACG842_002563"
     CDS             complement(1298..2134)
                     /locus_tag="ACG842_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0249049.1"
                     /translation="METLIESFGTIIKEIRKEQKMTQKMLSHDICSQSVLSRIENNEE
                     LPNVLVMMQICQRLGVTIDHVMNLSRKTTRTNDKLFELLDSCFQKRDYRKLEQVLKSS
                     DISENLYQATDFQRYYYYLGVCEFTLRQNISQSLYYLKEALSYSSQKERIHVSDTEIQ
                     LISCIGKIYGVAGKTAEARYYLSRSIQLLHQSADERSKSEFSQIFYNYGNFLFHQNEI
                     DEALEQVNQGIYWAQEKNSYYYLNDLFVLKSLIYKRKEEPERAAFYEGLAQAVKQISK
                     NL"
     gene            complement(2318..3535)
                     /locus_tag="ACG842_002564"
     CDS             complement(2318..3535)
                     /locus_tag="ACG842_002564"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HFQ0249050.1"
                     /translation="MREFEKSNKLEGVSYDVRGPVLEEAERMQEEGISILKLNTGNPA
                     PFGFEAPNEVIRDLIMNARDSEGYSDSKGIFSARKAIEQYCQLKHFPNVTINDIYTGN
                     GVSELITMCMQGLLDNGDEVLVPMPDYPLWTASIALAGGTPVHYICDEEAEWYPDIDD
                     IKSKITSRTKAIVIINPNNPTGALYPKELLEEIVEVARQNNLIIYSDEIYDRLVMDGL
                     EHIPIATLAPDLFVVTLNGLSKSHRVAGFRVGWMVLSGDKSHVKGYIEGLNMLSSMRL
                     CSNVLSQQIIQTALGGYQSVDELLLPGGRIYEQREYIYNAINDIPGLSAVKPKAAFYI
                     FPKIDTKRFNILDDEKFVLDFLHEHHILLVHGGGFNWNQPDHFRIVYLPKMDDLKQTA
                     KKMREFLATYRQK"
     gene            complement(3684..3845)
                     /locus_tag="ACG842_002565"
     CDS             complement(3684..3845)
                     /locus_tag="ACG842_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0249051.1"
                     /translation="MEKYDGEFSGLGMILGVLIGLAFGRFLLGFMLGIICGIAMDWAA
                     NLWNDYHDN"
     gene            3918..4001
                     /locus_tag="ACG842_002566"
     tRNA            3918..4001
                     /locus_tag="ACG842_002566"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3952..3954,aa:Leu,seq:gag)
     gene            complement(4241..5312)
                     /locus_tag="ACG842_002567"
                     /pseudo
     CDS             complement(4241..5312)
                     /locus_tag="ACG842_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327954.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         1653 a         1082 c          838 g         1739 t
ORIGIN      
        1 tttttactag aacacaaaaa aaaccttaaa accaaactta agaagtttag ttttaaggtt
       61 aacttaaatt atattatgcg tggaaacgtt ccacgccatc tagataagtt gcttccaatg
      121 tcatatcagg gttcaatacg ataaagtctg catcgcgtcc ttgagcgatt tttccacatg
      181 tatcatcgat tttacagctg attgctggaa tcagactagc catcatgact gcttgttctg
      241 gtgttgctaa tccccagtct acaacatttt taattgcttc tttcaatttc aatatacttc
      301 cagctaagtt gccagattct agacgtgctg ttccttcttt tactactact ggaaattcgc
      361 ctaaattgta atttccatca ggcatcccac cggccatcat acagtctgta attaatgcga
      421 catgatcatg tcctgctttt tccattagga ttcccgcagc atttggatgt acatgatggc
      481 catcacaaat caattcagaa aagacatgtt ctaaagataa taacgcaccc accatacctg
      541 gttcgcgatg attcaatcca cgcataccat tgaaagcatg gacaaataca cttgcgcctg
      601 cttcaacagc tttttgcgct tcttctagtg tagcattact gtgtcctaga gaaacaacta
      661 ctccttcttc agtcacttgt ttgacaaact cttctacacc ttgacgttcc ggtgctaaag
      721 caattttttt gattaaacca cctgaagctt cttgccattc atggaaagta tccaaatcag
      781 gatccccgaa ataacttggg ttttgtgccc ctttgtgttc ttctgtgaag aaaggacctt
      841 caaaatagat tcctttgatt ttagctcctg gtgcttcctg atacattttg ccgattgtct
      901 cagctacatc ttttaaacgt tctttgcttg acgtcaatgt tgttggtaaa aatgaagtca
      961 ctccacaaga taacagacct tcagacatca ctttgatgcc ttctgcatca ttatccatta
     1021 catcatgatt catatagccg tgaatatgtg tatctaccaa accaggagca atccatttgc
     1081 cgctttggtc aatgatgttg acttctccct caggtagttc tttggtgtat tcaccaaaaa
     1141 gtccatcggt aatttcaaga tagcctgcat gtttgacacc tgacgctaaa aagaatttgt
     1201 cagcatagat aaaagttctc attctattct ctccctttgt ttttctacct ctaaagtact
     1261 cttatttaga gaagaagtca agaatggtct acaccattta caaatttttt gaaatttgtt
     1321 tcacagcttg tgctaagcct tcatagaatg ctgctctttc tggttcttct ttacgcttgt
     1381 atattaagct tttaagcaca aataaatcat tgaggtaata gtagctattc ttctcttgag
     1441 cccaatagat tccttgattc acctgttcta acgcttcgtc gatttcattt tgatggaata
     1501 agaaatttcc ataattataa aaaatttgcg aaaattcact ttttgatcgt tcatcagctg
     1561 attgatgcag cagttggatg cttcgactca aatagtagcg tgcttcagcc gtttttcctg
     1621 ccacaccata tatttttccg atacaactaa tcagttgtat ttctgtgtca gaaacatgga
     1681 ttctttcttt ttggctagag tagcttaatg cttctttcaa ataataaagg gactgagaga
     1741 tattttgacg caacgtgaat tcgcaaacac cgagataata ataatagcgt tgaaagtcag
     1801 ttgcttgata cagattttca gaaatatcag aagattttag tacttgttct aacttccgat
     1861 aatcacgttt ttggaaacaa ctatccaaca gttcaaataa tttatcgttt gtccgagtag
     1921 tttttcgaga gagattcatc acatgatcaa tcgttacccc tagccgttga caaatctgca
     1981 tcatcaccaa tacattcggc agttcctcat tattttcgat ccgacttaat acactttgtg
     2041 aacaaatatc atgcgaaagc attttttgcg tcattttttg ttcttttcga atctctttaa
     2101 tgatcgttcc aaaggactcg attaacgttt ccataagtat cctccccaaa atatgcgtat
     2161 tcgcattcta ttcgtatttt actttaggaa gtcgcaaact tcaagaaaag acttgcaact
     2221 aaataatata ctttagttat ttaagaaaac tccactagaa agagagcctc agtgtgtttg
     2281 tctgtggccc tcctactgaa aaatttggtt tttaaaatca tttttggcga taggttgcta
     2341 ggaattctct cattttctta gctgtttgtt tcaaatcatc cattttagga agataaacga
     2401 ttcgaaaatg atctggctgg ttccaattaa atccaccacc atgtacaagt agaatatgat
     2461 gttcatgtaa aaagtccaaa acgaattttt catcatccaa aatattgaat cgctttgtat
     2521 caatttttgg gaatatataa aaagcagctt ttggttttac ggctgaaaga ccaggaatat
     2581 cattgattgc attatagata tattctcttt gttcatagat ccttccgcct ggcaggagca
     2641 gctcatcgac actttgataa ccgcctagcg cggtttggat aatttgctga gacaatacat
     2701 tggaacacaa acgcatggag gacaacatat tcagcccttc tatatagcct ttgacgtgtg
     2761 acttatctcc acttaggacc atccagccta ctcgaaatcc tgctacacga tgagatttgg
     2821 atagaccgtt cagcgtaaca acgaacaaat caggcgctaa tgtagcaatc ggaatatgtt
     2881 ctagaccatc catcactaag cgatcataga tttcgtcgga ataaataatc aggttatttt
     2941 gtcgagctac ttcaacgatt tcttctaata attcttttgg ataaagtgcc ccagtaggat
     3001 tattaggatt aatgataaca atagcttttg tccgactcgt tatttttgat ttgatatcat
     3061 caatatcagg ataccattct gcctcttcat cgcaaatata atgaacaggt gtgcctccag
     3121 ctaatgcaat agaagccgtc cacaacggat agtccggcat tggaaccagt acttcatctc
     3181 cattgtccag caacccctgc atacacatcg tgatcaattc gctcactccg ttgccggtgt
     3241 agatatcatt gatcgtcaca ttcggaaaat gtttcagttg gcaatattgc tcaattgctt
     3301 ttctagcaga aaagatcccc ttggaatctg agtagccttc tgagtcacgg gcattcatga
     3361 tcaaatcacg aatgacttcg tttggtgctt caaatccaaa aggtgcggga tttcccgtgt
     3421 tcaattttaa aatactaatg ccctcttctt gcatccgctc cgcttcttct agaacgggac
     3481 cgcgaacatc gtaactgaca ccttctaatt tgttggattt ttcaaattct ctcatcactt
     3541 agccaatccc tcatttcaaa aagttagaac tttccaaatt ttttgttatt tactatttca
     3601 atatcatact ccatcctaga aaattttgcc acttatttag gaaaaacgta agtattaaaa
     3661 aaacacaaac aagcgttgag aacttaattg tcatgatagt cattccatag attcgctgcc
     3721 caatccattg caattccaca aatgattcct agcataaaac caagcaaaaa acgtccgaat
     3781 gcaagtccaa tcaatacacc taagatcatt cccagtcctg aaaattctcc gtcatatttc
     3841 tccatcttaa taagtcctcc catttttgag cttgacaact atgtaaaacc tcgctatcat
     3901 tagtgtgtta ttattacgca gatgtgttgg aattggcaga caagcatgat tgagggtcat
     3961 gtgagcgtta gctcgtgtgg gttcgagtcc catcatctgc actacaactc gtgaagttaa
     4021 ttttgcttca cgagtttttt tgtttgaacg tttctttatg ccattatata catatcactg
     4081 tatcctttac aaaaagaacg tacaatcatg taaacagcct gaggtgagta tatgaaggtc
     4141 ggaaacaagc tactcgtaga aacttgcaag tgttaaaaca attaatcgaa aaacgagtaa
     4201 aatccctcac ctaatactaa agcgagggat tttttgtata ttatctgttt atcaccgtta
     4261 ttataaaggt acaaatacca agcacggtta atccaaattg cataaattca tttttttgta
     4321 cactcatgct tttcattagc tctttaactg ccattagtac taagatggcg ataacaacat
     4381 aaagtatata gtacgtccgc attccggaag cccatactgt gggagaaaat cccataatta
     4441 accttgaaca aaatcccatt atcatcaaaa taactaaaaa aatagaagtt ttcttattgt
     4501 caaaacttaa ataaattcca acaattaaac atagtaataa tgccaagact actaaagtag
     4561 ctatccaagt acctggataa aaaagtgaca gtttcgttcc taactctgta aagagattat
     4621 tcaggttact actactccaa atcattgccc ttttatttcc attgacgtaa gtaaagcttt
     4681 gacccattgt attaccaaaa taaataatca ggtttaagaa aaacggtaat gcagtcaata
     4741 tacgcacata atagttttga catttcctat atgttagaag gaaaattaag aaaaatagca
     4801 ataaaaataa gatattcatg tctaaaaaaa cggctttcca aatgatgaaa tacctaaatc
     4861 taacttatta agaaagttga aatttttata ttcaggaaac catttattaa tttcatgtgc
     4921 agcacgtaat gcattccctg gagttgtcaa agagaaaatt aattctgcta agcttattat
     4981 tgaaaatact gataacttat aattatatcg tccattgaca atcaaatata aactaactat
     5041 acttgttaga acaaaaaaac aggcgttgac ttgttcttga ttagttaaaa atatcaataa
     5101 aggaatactt acccaatata tttttctatt tatttcttct cctcttaaga gttgaaaaaa
     5161 cggataaaaa gttagtaaac taaatgctac tggccactga tagttcaagg tggttgctct
     5221 ccaaccggtt tcccccatta ctgtcaatgg tattaaacag tatatagagc aaaccaataa
     5281 taaattttta acatttattt tctcaaaaac at
//
LOCUS       DAZKLM010000127         5310 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3764
            SAMEA115706913-rid22582563.denovo.132, whole genome shotgun
            sequence.
ACCESSION   DAZKLM010000127 DAZKLM010000000
VERSION     DAZKLM010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706913
            Sequence Read Archive: ERR13196647
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5310)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5310)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287823.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287823.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,004
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,849
            CDSs (with protein)               :: 2,849
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 41 of 96
            Pseudo Genes (incomplete)         :: 52 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 15 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5310
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706913-rid22582563.denovo.132"
                     /isolate="VRE3764"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            97..1104
                     /locus_tag="ACG842_002568"
     CDS             97..1104
                     /locus_tag="ACG842_002568"
                     /EC_number="4.2.1.115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050377 - UDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 4,6-dehydratase family
                     protein"
                     /protein_id="HFQ0249052.1"
                     /translation="MFKGKTILVTGGTGSIGSEIVRQLLKYEPKAIRIYSRGEEKQFY
                     MQQELSNHTNLRFLIGDIRDSQRIDYACRDVDIIFHAAAMKHVPASEYNPMEAVKTNV
                     MGTQNVIDAAIKNNVKHFVGISTDKVVSPTNTMGATKLLSEKLIIAANQYKGKTETIF
                     SCVRFGNVMGSSGSVIPLFSKQIKNNKDITLTDVDMTRFMMSIPDAARLVLSAAMNSK
                     FGETYVLKMPAINILTLAQSLIKIYNDTHDEKYRGEIKVVGIRPGEKLYEELMTTDEI
                     ERSYENDKMYIIPPHYHATDELYKDYEKIKITAYSSDATELMSGQEFIRIAKENNVVT
                     W"
     gene            1116..2120
                     /gene="neuB"
                     /locus_tag="ACG842_002569"
     CDS             1116..2120
                     /gene="neuB"
                     /locus_tag="ACG842_002569"
                     /EC_number="2.5.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0016051 - carbohydrate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase"
                     /protein_id="HFQ0249053.1"
                     /translation="MKSEVYVIAEIGVNHNGSLDLALESIDKAKECGANAVKFQTFKT
                     NKLVSKKAPMAKYQKDNLKEDSSQFEMLKKLELSQSDFIRIKDYCIQNKIDFLSTPFD
                     EESADFLKQIGVQGFKIGSGDLTNLPFLRKIDMYGLPVLLSTGMADIHEVSEAVDCFI
                     DSPVTILHCTSNYPASSEDINLLAINTLKEMFGIPVGYSDHSLGYDVGICAVAMGAKV
                     IEKHFTLDKELPGPDHKASLDPQEFADFVKHIRNTEIFLGDGVKRAMPSELSTKEVSR
                     KSLVVTENLNVGDILTEKSLTIKRPGNGIAPKFLYQYIGKKVKRPISADSVLLEEDVE
                     "
     gene            2117..3265
                     /gene="neuC"
                     /locus_tag="ACG842_002570"
     CDS             2117..3265
                     /gene="neuC"
                     /locus_tag="ACG842_002570"
                     /EC_number="3.2.1.183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0006047 - UDP-N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase"
                     /protein_id="HFQ0249054.1"
                     /translation="MKKILIVSGTRADYGIYKPVAENLLQDNWDVSFVVSGMHLSHKY
                     GFTKELILADGFRISGEVQSLFLENNKGNMARSVALEINGFTNIFEQTSPDFVLLLGD
                     RGEMLAAAIACLYLGIKTAHIHGGEVSGTVDESIRHAITKIASVHFAATEKSKERLIK
                     MGEDSWRVFPVGAPRIDTILNNRLPSFRDIKEKYHLKFEKKKYVLQVFHPVVTELENI
                     EKQILALINSMEQQSQPIICILPNSDAGSEIIRKMYQKYLKTDIIYITNLEPEEYLTI
                     LKECKFMIGNSSSGIIEAASFKIPVINLGTRQNGREQSKNIINANLNENEIETALNKI
                     QDPQFIKSLKNVKNIYGDGNSSRRITKTLNQILNDGEDIKWIQKQIAY"
     gene            3238..3915
                     /locus_tag="ACG842_002571"
     CDS             3238..3915
                     /locus_tag="ACG842_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HFQ0249055.1"
                     /translation="MDSKTNSLLKYTSDPYIIIGAGGHAKVIIETLRLENKLIYGFFD
                     DKQNKMQDLKYLGKINELNSWADSKKKIYKFIVAIGDCVVRKKIIKKLNMPSEQYGIA
                     VHPSAIVAKEISIGYGTVIFANAVINPDAVIGEHAIINTGSIVEHDCRINNYVHLSPG
                     VCLAGGVHVGVGTQIGIGSQCIQMKKIGSWCMIGAGSTIVKDIPSHSLAYGNPAKIKK
                     EGINFEF"
     gene            3866..5047
                     /locus_tag="ACG842_002572"
     CDS             3866..5047
                     /locus_tag="ACG842_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HFQ0249056.1"
                     /translation="MAIQLKLRKKELILNSRILLSSPHMGGTEEKYVAKAFKDNWIAP
                     LGENVDKFEESITKYTGSKGGASALSSGTAAIHLALRLLNVGKNDIVFAPSFTFIATV
                     NPILYQGAVPVFIDSEPNTWNMSPVALESALEEAERNNQLPKAIIVVNLYGQMADYER
                     ILAIANQFNVPVIEDAAESLGSTYKGISSGLFGTIGIYSFNGNKIITTSGGGALVSSD
                     KKYVEHSRFLATQARDQAPYYQHSEIGYNYRLSNISAGIGRGQMEVLNDRVAKRREIF
                     NRYKEAFSGIDRIEMMPEVDGMVPNRWLSTLTINDYNSIDQVVNVINKLNEKNIETRA
                     LWKPLHLQPVFQGVKMYYHDNNLSVSEKLFNSGLCLPSGSNMTDSEQEFVIDEFKKIF
                     K"
     gene            5060..>5310
                     /locus_tag="ACG842_002573"
     CDS             5060..>5310
                     /locus_tag="ACG842_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFQ0249057.1"
                     /translation="MNIGIIGLGSMGKRRLRLIQEHFNDILLCGVDTNEARRKEVEKE
                     FGIPTYNSLTEAKNLFEINSVFVCTSPITHEFIIKEALN"
BASE COUNT         1968 a          682 c         1040 g         1620 t
ORIGIN      
        1 gttttgacat cattattgat aattagttta gttatatatt ttttaaagat taagaatcga
       61 attatagatg actataattc ataggaggag atataagtgt ttaaaggaaa aacaatatta
      121 gtaactggcg gaactggttc cattggaagt gaaattgttc gtcagctttt aaaatatgaa
      181 ccaaaagcta tcagaattta tagtagaggt gaagaaaaac aattttatat gcaacaagag
      241 ctctcaaatc acactaattt gagattttta attggtgata tcagagactc tcaaaggatt
      301 gattatgcat gtcgtgatgt ggatattatt tttcatgctg ccgcaatgaa acacgttcca
      361 gcaagtgaat ataatcctat ggaagctgta aaaacaaatg tgatgggaac tcaaaatgta
      421 atagatgcag cgatcaaaaa taatgtaaaa cattttgtag gtatcagtac agataaagta
      481 gtgagtccta ctaacaccat gggtgcgaca aagttattga gtgaaaaatt aataatagct
      541 gccaatcagt ataaaggaaa aacagaaaca atattttctt gtgtaaggtt tggaaatgtc
      601 atggggtcaa gtggatctgt tattcctctt tttagtaaac aaataaaaaa caataaagat
      661 attactttga cagatgttga tatgacacga tttatgatgt caataccgga tgctgcaaga
      721 ttagttctgt ctgctgctat gaattcaaaa tttggagaaa catatgtctt aaaaatgccg
      781 gcaatcaata ttttaacttt agctcaatca ttaataaaaa tatataatga tacacatgat
      841 gaaaaatata gaggtgaaat aaaagttgta ggtatcaggc caggagaaaa attatatgaa
      901 gagctaatga ctacagacga aattgaacgc tcatatgaaa atgataaaat gtatatcatt
      961 ccaccacact atcatgctac tgacgaactg tacaaagatt atgaaaaaat taaaatcact
     1021 gcatactctt cagatgcaac agaattaatg agtggacagg aatttattag aatcgcaaaa
     1081 gagaataatg tcgtgacttg gtaaggagtt ttatgatgaa atcagaagta tatgtaattg
     1141 cagaaattgg tgtaaaccat aatggaagtc tagatttagc tttagaaagt attgataaag
     1201 ctaaagaatg tggagctaac gcggttaaat ttcaaacatt taaaacgaat aagctagttt
     1261 ccaaaaaggc tccaatggca aaatatcaga aggataatct taaagaggac agttctcaat
     1321 ttgaaatgct aaaaaaatta gaattatctc aatctgattt tattagaata aaagattatt
     1381 gtatacaaaa taaaattgat tttttgtcta ctccttttga tgaagaaagt gcagactttc
     1441 taaaacaaat tggtgtacaa ggatttaaaa ttggctcagg agatttaaca aatttacctt
     1501 ttcttagaaa aatagatatg tatgggttgc cagttttatt atctacaggt atggctgata
     1561 ttcatgaggt cagtgaggct gtagattgtt tcatagatag tccagttact attttacatt
     1621 gtacttcaaa ttatccggct agttctgaag atattaattt attggcgatt aacacattaa
     1681 aagaaatgtt cgggataccc gttgggtact ccgatcattc attgggatat gatgttggta
     1741 tttgtgctgt tgcgatgggt gctaaggtaa ttgagaaaca ttttaccttg gataaggaac
     1801 tacctggtcc agatcataaa gcttctttag atcctcagga atttgcagat tttgtaaagc
     1861 atattagaaa cactgaaata tttttaggtg atggtgttaa gcgagcaatg ccttctgaac
     1921 ttagtacaaa agaagtatca agaaaaagcc tagtggtcac tgagaattta aatgtgggag
     1981 atattcttac agagaaatct cttacaataa aacgtcctgg aaatggaata gctcctaagt
     2041 ttttatatca atatattggc aaaaaagtta aaagaccaat aagtgcagat agtgttctgc
     2101 ttgaggaaga tgttgaatga aaaaaatact tatagtaagt ggaacacgag ctgattatgg
     2161 aatatataaa cctgttgctg agaatttact acaagataat tgggatgtat catttgttgt
     2221 ttctggaatg catttatccc ataaatatgg atttactaaa gaattgatct tagctgacgg
     2281 atttagaatt agtggagaag ttcaaagttt gtttttagag aacaataagg gaaacatggc
     2341 tcggtctgtg gcattagaga taaatggttt cacaaatata tttgaacaaa ctagccctga
     2401 ttttgtatta ttactagggg atagaggaga aatgttggca gctgcgatag cttgtttgta
     2461 tcttggaatc aaaacagcac atattcacgg aggcgaagtg tctggtactg tagatgaatc
     2521 aataagacat gccataacga aaatagcatc tgttcatttt gctgctacgg aaaaaagcaa
     2581 agagcgttta ataaaaatgg gggaagattc ttggagagtt tttcccgtcg gagctcctag
     2641 aatagatact attttaaata atagattacc tagttttaga gatataaaag aaaaatatca
     2701 tctaaaattt gaaaagaaaa aatacgtttt gcaagtattt catccagttg taaccgaact
     2761 agaaaatata gaaaaacaaa ttttagcttt gatcaattca atggaacaac aatctcaacc
     2821 aattatttgc attttaccta atagtgatgc tggtagcgaa attatcagaa aaatgtacca
     2881 gaaatattta aaaacggata ttatttatat tacgaattta gagccggagg aatacctgac
     2941 tatattaaaa gagtgcaaat ttatgatcgg aaattcttct tctgggataa ttgaagctgc
     3001 atcattcaaa ataccagtaa taaatttagg aaccagacaa aatggacgag aacaatctaa
     3061 aaatattata aatgcaaatc taaatgaaaa tgaaattgaa actgctttga ataaaataca
     3121 agatcctcag tttataaaaa gcttgaaaaa tgttaagaac atctacggtg acggcaacag
     3181 ttctagaaga ataacaaaaa cattgaatca aatattgaat gatggggaag atattaagtg
     3241 gattcaaaaa caaatagctt attaaaatat actagtgatc catacattat tattggggct
     3301 ggcggacacg caaaagtcat tattgaaacc ttaagattag aaaataagtt aatatatgga
     3361 ttttttgatg ataagcaaaa taaaatgcaa gatctgaaat atcttgggaa aataaacgaa
     3421 ttgaatagct gggcggatag caaaaaaaaa atctataaat ttattgtagc tattggtgac
     3481 tgtgtagtaa gaaaaaagat tataaaaaaa ttgaatatgc caagtgagca atatggaata
     3541 gctgttcatc caagtgctat agtggcaaaa gaaatttcaa ttggttatgg aactgttatt
     3601 tttgcgaatg ctgtaattaa cccagatgct gtaattggag agcatgctat aataaacaca
     3661 ggtagcattg ttgagcatga ttgtagaatt aataactatg ttcatttatc tccaggtgta
     3721 tgcttagcgg gtggagtaca tgtaggtgta ggaactcaaa taggaattgg ttctcaatgt
     3781 atccagatga agaaaattgg atcttggtgt atgataggcg cagggagcac tattgttaag
     3841 gatattccct cacattcctt agcttatggc aatccagcta aaattaagaa agaaggaatt
     3901 aattttgaat tctagaattt tattatcaag tcctcatatg ggaggaacag aagaaaaata
     3961 tgttgctaag gcttttaaag ataattggat agcgccatta ggtgaaaatg tggataaatt
     4021 tgaagaaagt attacaaaat atactggttc taagggcgga gcttctgcgt taagttcagg
     4081 tacagctgcc atacatcttg ccttaagact gcttaatgtt ggaaaaaacg atattgtctt
     4141 tgctccttca tttactttta tagctacagt aaatcctatt ttatatcaag gggctgtacc
     4201 agtgttcatt gactcagaac ctaatacatg gaatatgagc cctgttgcac tagagagcgc
     4261 attagaagaa gctgaaagaa acaaccaatt acctaaggca atcatcgtag tgaatttata
     4321 tggtcaaatg gctgattatg aacgaatttt agctattgca aaccaattta atgttccagt
     4381 gatagaagat gcagcggaat cgttaggttc tacttataag ggaatttcgt caggcttatt
     4441 tgggacaatt gggatctatt catttaacgg taataagatc atcacaacct caggtggagg
     4501 agctctagtg tctagtgata aaaaatatgt tgagcattca agatttttag caacacaagc
     4561 aagagatcaa gcaccgtatt atcaacatag cgaaataggt tataattacc gtctaagcaa
     4621 tatttctgcc ggtatcggac gtggacaaat ggaagtattg aatgatagag tagctaaacg
     4681 aagagaaatt ttcaatcgat ataaggaagc tttttctgga attgatagaa ttgaaatgat
     4741 gccagaagtg gatggcatgg tgccaaatcg ttggttgtct actttaacga tcaatgatta
     4801 taattctatt gatcaagtag ttaacgtaat aaataaacta aatgaaaaaa acattgaaac
     4861 aagagctttg tggaaaccac tacatttgca accagtattt caaggggtaa aaatgtatta
     4921 ccatgataat aatcttagtg tgtcagaaaa gttatttaat agcggcttat gcttaccttc
     4981 tggaagtaat atgacagata gtgaacaaga atttgttatt gatgagttta aaaaaatatt
     5041 caagtaggtg tgatatttta tgaacatagg aattattggt ttaggttcca tggggaaaag
     5101 aagacttaga ctcattcaag aacactttaa tgatatttta ctatgtggtg ttgatactaa
     5161 tgaggcgaga cgaaaggaag ttgaaaagga atttggaata ccaacttata atagtctaac
     5221 agaggcaaaa aatttgtttg aaataaattc agtttttgtt tgtacatccc ctataaccca
     5281 tgaatttatt ataaaagagg ctttaaattt
//
LOCUS       DAZKLM010000128         5273 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3764
            SAMEA115706913-rid22582563.denovo.133, whole genome shotgun
            sequence.
ACCESSION   DAZKLM010000128 DAZKLM010000000
VERSION     DAZKLM010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706913
            Sequence Read Archive: ERR13196647
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5273)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5273)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287823.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287823.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,004
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,849
            CDSs (with protein)               :: 2,849
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 41 of 96
            Pseudo Genes (incomplete)         :: 52 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 15 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5273
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706913-rid22582563.denovo.133"
                     /isolate="VRE3764"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..273)
                     /gene="prgO"
                     /locus_tag="ACG842_002574"
     CDS             complement(<1..273)
                     /gene="prgO"
                     /locus_tag="ACG842_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001196543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA segregation protein PrgO"
                     /protein_id="HFQ0249058.1"
                     /translation="MALLPTNKDKKKIGAEDISIKDDINVELGERKYTAKERKPVQVD
                     PPVLKTIRDLSYAKDIPMYEIVKLAMDAYVDTLNDEERIIYNRRSKD"
     gene            complement(245..1198)
                     /locus_tag="ACG842_002575"
     CDS             complement(245..1198)
                     /locus_tag="ACG842_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFQ0249059.1"
                     /translation="MENLRTAFKTSDKLGKVVMLTGRKGGIGKTTDNDLLAIVSSQLF
                     EKDVLLIDYDQQRNTTSNIGSTYQITSFDRSMSAAIKKGDWVSGITQVSPHLYIMAGS
                     PGSEELNEYLSEKYPDRRKRSLAFIKPLEELRKNFDYIFIDCPPSTDNVVRAFLTAAD
                     YIIAMQELKRYAMEGTEDFINKVLVPIVTNFEESHLQIIGILPVLFSVRRSSQHANYQ
                     KTIDKYGESNIFKSIVKGSDRLEMYGENGIQLNDYVDRRWWAIFADIFTELEERIEYY
                     EQHGDIEGFDYTPKYADSMANHILPLGKEIKLNGIITNEQR"
     gene            1810..3303
                     /locus_tag="ACG842_002576"
     CDS             1810..3303
                     /locus_tag="ACG842_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primase C-terminal domain-containing protein"
                     /protein_id="HFQ0249060.1"
                     /translation="MLSEIYSAILKNGLRTYKYRNSTMKPAGHIDSSRSGAIFAYRSK
                     QLMNVGRGMVITSEEAILENEKKLTHWTPNTYRFGTYADDYRTVVKGHSEKNLSQINT
                     FVVDIDSKENHQGEIILACLDQVGYMPTLILESDHGYQVYFVLKTPGYVTKKSNFKVI
                     DVAKKISKTIRLQLAEKLSGVDLGCNHFGIARFPQENNIVFKEMNYQYSFADWIQWSM
                     KLESDQKTKEDRERKLIVFPEKKEIRQVDEPWFDMLLHKANIVGGEGRLGRNNVIFTL
                     SLAYYSSGYALDTCEYNMFTFNERLQEPLSEAELVKIVRSAYSGNYQAASREFIIELC
                     QQWVSPTLKNNDLFIQRRGWWKFKKPRDQRKYSHKNEWKEDLLAYLSEKSLASEPYLN
                     LSKKELASQLTMPKRTLDKILNELREENKVFYKVTRGRNGGMIIASVKTLFARVIQLS
                     KEKRLDYSTRITEVFGLNESLVEETFRQLSTPLNPLKEVNLFEVDTG"
     gene            3438..3734
                     /locus_tag="ACG842_002577"
     CDS             3438..3734
                     /locus_tag="ACG842_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HFQ0249061.1"
                     /translation="MSLKNYVYSHPVNVFIIGRLGLSVEVFCELYGFKQGTLSSWVTR
                     EKTVASLPIEFLHGLSLASGETMDQVYDCLCVLEQEYIEYKIANELRKRKKYIQ"
     gene            3835..4686
                     /locus_tag="ACG842_002578"
     CDS             3835..4686
                     /locus_tag="ACG842_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0249062.1"
                     /translation="MKSDDWIEHTWLPLKRNSKRKKFWVLQLWPLGKLCSYGARLILD
                     LTVYSIGVFGTAYYLINDPNRIAASDIFTVLMTLIFVMLLSIFTQLVMQFTRQKLTAG
                     KLFKSLFDLIVVNAILATLIKQIEQSGRTTKGVKDFLLTFIHFVNGHMYLFEVGIILF
                     FVGYVSTKLIKRSIEKEIEIDLIPTLLGYEYNVDFGRYDELINTDIVHTSIHESTVKG
                     IRSILKNGQEMKAIDRRKEIYNFYEVEASLRYGIGKLWLDGSNIRQNKPYIFWNSSDL
                     ELPMKDK"
     gene            4707..4919
                     /locus_tag="ACG842_002579"
     CDS             4707..4919
                     /locus_tag="ACG842_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0249063.1"
                     /translation="MIPLYRIVLIVGMELKTLKKEQLFKVKERHNQLLCSKTGTMNIE
                     YRTEYLELTNQTDFLLIRKQQEQEKN"
BASE COUNT         1799 a          821 c          997 g         1656 t
ORIGIN      
        1 atcttttgat cgtctattat atataatccg ttcctcgtcg tttaaagtat caacataggc
       61 gtccattgca agtttgacta tttcatacat aggaatatct tttgcataac taagatcccg
      121 aatggttttt agtacgggag gatctacttg aactggtttt ctttctttcg cagtgtactt
      181 tcgttcccct aactcgacat taatatcatc tttaattgaa atatcttcag caccaatttt
      241 cttcttatct ttgttcgttg gtaataatgc catttagttt aatctccttt ccaagaggta
      301 ggatatgatt ggccattgag tcagcatatt taggtgtata atcaaatcct tcaatatcac
      361 catgttgttc atagtattca attctttctt ctaattctgt aaagatgtct gcaaaaatag
      421 cccaccatct tcgatctaca taatcattta attggatacc attctcacca tacatttcta
      481 accgatccga gcctttaaca atgcttttaa aaatgtttga ttcaccatac ttatcaattg
      541 ttttttgata gtttgcgtgt tgagaggaac gtcgcactga gaaaagaaca ggcaaaattc
      601 caatgatttg aagatgagac tcttcaaaat tcgtgacaat cggaactaaa actttattaa
      661 taaaatcttc tgttccttcc attgcgtacc gttttaattc ttgcattgca ataatataat
      721 ctgcagcagt taagaatgca cgaaccacat tatctgttga tggtgggcag tcgataaaga
      781 tataatcgaa attttttctc aattcttcta aaggtttaat gaaggccaat gatctcttac
      841 gacgatcagg atatttttcg gacaagtatt cattaagttc ttcagatcca ggagagccag
      901 ccataatata taaatgtgga cttacttgag taatacctga aacccagtcc ccttttttga
      961 ttgctgcaga catggatcga tcaaagctag taatttgata cgttgatcca atgttggaag
     1021 tagtatttct ttgttggtca taatcaatta gcaacacgtc tttttcaaaa agttgactag
     1081 aaacaatcgc taataaatca ttgtctgtag tcttaccaat gccgcctttt ctacctgtaa
     1141 gcattacaac ttttcctagt ttatctgatg ttttaaatgc agtccgtaag ttttccatag
     1201 ttccctccaa tttcctatac taaatatatt tagtatactt catatactta atatattcga
     1261 tatatctaat atactccaaa ttgataagaa ttcagacttt ataaggaata tagtggatat
     1321 actaaatata tttgctatat tcagtatatc agatatattc aaaatgtcaa aatatatttg
     1381 tgcgttttgc gttgacaatt tagagtgaaa agaggtaaac ttaattctat agaaaccaca
     1441 gataattcac tatttacaaa gtagcaaaag cagtggtact ctttaaacag aaaaattaca
     1501 taaaaaaaag agacggaaat aattgatttt agcagaatca attatttctg aatcacatgg
     1561 ttaggacatg tgttcacgct cgtctcaaga atatgtaagt agctaccaac tacttatatt
     1621 aaaagttgat cggatacctt ttccgattgc ctttattcta ccaaaaaaag gcgagcaact
     1681 caacagaaga acgtttattt agtgtactta aattaaatat tcttaaacac tgaagagaga
     1741 acacatacta tggctgagat taaagctaaa ctgtgttctc tttttgtttg agaaaggacg
     1801 ttacaatcga tgttgagcga aatttattca gctattctta aaaacggctt acgtacatac
     1861 aaatatcgaa acagcaccat gaaacctgca ggtcatattg attccagtag gagtggtgct
     1921 atctttgcgt accgtagcaa gcaattaatg aatgttgggc gtggtatggt cattacttcg
     1981 gaagaagcta ttttagagaa cgaaaagaaa ctaacgcatt ggacacctaa tacctatcgc
     2041 tttggtactt atgcggatga ttatcgaaca gtggttaaag gtcattctga gaaaaactta
     2101 agtcaaatca acacatttgt agttgatatc gattcaaaag agaaccatca aggcgaaatt
     2161 attttagcct gtttagatca agtgggctac atgccaacgc taattttaga atcagatcat
     2221 ggttatcaag tttattttgt actaaagaca ccaggctatg tcacaaagaa aagcaatttc
     2281 aaggtgatcg atgtagcaaa aaaaatttca aaaacaattc gtctacagtt ggctgaaaaa
     2341 ttgtccggag tggatctagg atgtaaccac ttcggaattg ctcgattccc acaagaaaat
     2401 aatattgttt ttaaagaaat gaactatcag tattcttttg ctgactggat tcaatggtcg
     2461 atgaagcttg aatcagatca aaaaacaaaa gaagatcgag aacgaaaact aattgtcttt
     2521 cctgagaaaa aagaaattcg tcaagtagat gaaccgtggt tcgatatgtt acttcataaa
     2581 gcaaacatcg tcggcggaga aggacgtctt ggtcgaaata atgtgatttt cacgctttct
     2641 ttagcttatt actcttcagg ttatgcattg gatacttgcg aatacaatat gttcacattc
     2701 aacgaacggt tacaggaacc gctatctgaa gcagaattag taaagattgt acgtagtgct
     2761 tattctggta attatcaagc agcaagcaga gaattcatta ttgaattgtg ccagcaatgg
     2821 gtttcaccaa ccttaaaaaa taatgatctg ttcattcaac gacgtggctg gtggaaattt
     2881 aaaaagccac gagatcaaag aaaatatagt cataaaaatg aatggaaaga agatctattg
     2941 gcttatctaa gcgaaaaaag tcttgctagt gagccttatt tgaacctgtc aaaaaaagaa
     3001 ttggcttcac aactaacgat gcccaagcga acacttgata agatcttaaa tgagttacga
     3061 gaggagaata aagtcttcta caaggtaaca cgaggaagaa atggcggaat gatcattgct
     3121 tcagtcaaaa cgttgttcgc gagggttatt caactaagta aagaaaagag actagattat
     3181 tcaacaagga tcacagaggt atttggactc aacgagagct tagtagaaga gacatttaga
     3241 caattatcaa cccccttgaa ccctcttaaa gaagtgaatc tttttgaagt agatacaggt
     3301 tgaaaaataa aaggtgcaaa ttgccctata caatatatct atgatacttc tgtaggtgtt
     3361 gctccctttg cctagctacg tgtaggtggt atacttataa ctgcgtatgc agtcttatgt
     3421 atcatggagg aagttgtatg tcgttaaaaa attatgtcta ttcacatcct gttaatgtgt
     3481 ttatcattgg tcgtttggga ttatctgtgg aagtgttctg tgagctttat ggttttaagc
     3541 aggggacttt gtcttcttgg gttaccaggg aaaaaacagt tgcttctttg ccgattgagt
     3601 ttcttcatgg gttgtcctta gcttctgggg agactatgga tcaggtatat gattgtttgt
     3661 gtgtgttaga gcaggagtat attgagtata aaatagctaa cgagttaaga aaaaggaaga
     3721 agtatatcca gtagtagttt ttatttactt aaaaaattgg ttcattgagc aacctatcgc
     3781 tgctaaagta gcaaaggaaa acgttaaagg aaagaattta gggaagaaat cgaaatgaaa
     3841 tcggacgact ggattgaaca cacctggctt cctctgaaaa gaaatagcaa acgaaagaaa
     3901 ttttgggtat tgcaattatg gccattagga aaactttgta gttacggggc acgacttatt
     3961 cttgatttaa ccgtttactc gattggagta tttggaacgg cttattactt gataaatgat
     4021 cccaatagga ttgcagcatc agacattttt acagtgctaa tgacgttgat ttttgtcatg
     4081 ctattgtcta tttttactca attagtcatg caatttacca ggcaaaaatt aacagcagga
     4141 aaacttttca aaagcctatt cgatttaatt gttgtaaatg caattctagc gactttaata
     4201 aaacaaattg aacaatctgg aagaacaaca aaaggagtga aggatttttt attgacgttt
     4261 attcactttg taaatggaca catgtatttg tttgaagtgg gtattattct cttttttgtc
     4321 gggtatgtat ccacgaaact tattaaacgt tcaattgaaa aagaaattga aattgacctg
     4381 attccgacac ttcttggtta tgagtacaat gttgattttg gaagatatga cgaattgatc
     4441 aatacggata tcgttcacac gagtatccat gaatctactg ttaagggcat cagatctatt
     4501 ttaaaaaatg gccaggaaat gaaagcaata gatcgaagaa aagagatcta caatttttat
     4561 gaagtcgaag caagcctacg atatggcatc ggaaaattgt ggttagatgg aagtaatatt
     4621 cgtcaaaaca aaccatacat tttttggaac agtagtgatt tagaacttcc aatgaaagat
     4681 aaataattat ggagaatcgt ttgaaaatga ttcctctata caggattgtg cttatagttg
     4741 gcatggagtt aaaaacctta aagaaggagc agttattcaa agtgaaagaa cgtcataatc
     4801 agttactatg tagtaaaacg ggtacgatga acattgaata ccgaacagaa tatttagaat
     4861 taacgaatca aactgatttt ttgttgattc gaaagcaaca agagcaagaa aaaaattaat
     4921 ggctacttat agccaattga ttgatgaatg ggaacaattc aaggaatcta aaattccaga
     4981 acgtctctaa aagcctttat ttgtaaagag cataaggaaa cgtgggaaca ctttttaaaa
     5041 aaagtgttcc aataggctgt gcctattacg tttctcaaaa ataaatgtgg gaacaaaata
     5101 tttaaaaata ggatagtgaa aggatagtga aggatagtaa aaggagggta gtaggatagc
     5161 aaaaaggata ggctcctttt cgctatcctt tttaaattgg aggaattaat atggaagtaa
     5221 gaataagaaa tgtggatgag aaaatcgtaa gagaaattga tcgaagatgt gac
//
LOCUS       DAZKLM010000129         5223 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3764
            SAMEA115706913-rid22582563.denovo.134, whole genome shotgun
            sequence.
ACCESSION   DAZKLM010000129 DAZKLM010000000
VERSION     DAZKLM010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706913
            Sequence Read Archive: ERR13196647
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5223)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5223)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287823.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287823.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,004
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,849
            CDSs (with protein)               :: 2,849
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 41 of 96
            Pseudo Genes (incomplete)         :: 52 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 15 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5223
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706913-rid22582563.denovo.134"
                     /isolate="VRE3764"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(397..567)
                     /locus_tag="ACG842_002580"
     CDS             complement(397..567)
                     /locus_tag="ACG842_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0249064.1"
                     /translation="MTKKHLLFGSILLGILSFSPMLLSVANKGNKKGELKLNPNYYNS
                     QNEEVFVEKLDK"
     gene            complement(582..1115)
                     /locus_tag="ACG842_002581"
     CDS             complement(582..1115)
                     /locus_tag="ACG842_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HFQ0249065.1"
                     /translation="MQPQETFTIKELSDLFKMSRQAISKHIQKLDSSMIAKNERGYKV
                     VLRSGVLQLARNLDNQRLLDKLHKHEQNNDKNAYIPNKNVVANKLLDQLREENEFLRE
                     QLALKEQNNSHIQKLLDQQQQLTLQANQQIEKLQEQLQLTYTEESSENSSTTLSEATE
                     TMDEQTKNWKKWWQLWK"
     gene            complement(1136..2080)
                     /locus_tag="ACG842_002582"
     CDS             complement(1136..2080)
                     /locus_tag="ACG842_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HFQ0249066.1"
                     /translation="MANEIVKYHHELNTIPLRKFTPVEMNLFFSIVSRMREKGDKTVR
                     FTFDQLKDLSNYKATANVRFVEDLKATYEKILSLRFGRRSESGLSYDMFVMFTEFKIN
                     GDVENPYVDIRIYEKALPLLNNLDEWVRYSLQQFNELQSGYAKTMFRLLKQYRTKGFA
                     YFSKEDFLELLDIPKSYKQPDIDKRVIKPIRQELTAIFKGLTIKKKYGKGRGKPVIGY
                     QFTFKPEIKKADDFNKHAPTKEDQNKALALADKQKEYEEPATTINEKIKQKKRELELL
                     QQIKQLEKEKKALEKQTESALSEKEKKSLLSDLDNLFQ"
     gene            2717..2803
                     /locus_tag="ACG842_002583"
     CDS             2717..2803
                     /locus_tag="ACG842_002583"
                     /inference="COORDINATES: protein motif:HMM:NF033608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HFQ0249067.1"
                     /translation="MFELFICPLLIEVCVSLFSYWLNNRNKK"
     gene            complement(2924..3613)
                     /locus_tag="ACG842_002584"
     CDS             complement(2924..3613)
                     /locus_tag="ACG842_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0249068.1"
                     /translation="MAFYLPYLLIFASILGIIWLSYKAYQTRYSLKGSKIRFKRFLLP
                     GVIFTLSIVLSGLFGVLEGNKRISRSILLGDITQKYEAACDKKKKEQVLTQKMEEFTT
                     CYEEMNDIFVNQEKRLTDKNMEKLTRLYQNLPEKQQKEVQDNYERTKKDVQYFKDTQT
                     EEACYNLFSDTIPFSTSEQERKERQQSVTYERYKALLQQATNIQNPTKKETALNYLKS
                     VKEWLDQQQQN"
     gene            complement(3620..3898)
                     /locus_tag="ACG842_002585"
     CDS             complement(3620..3898)
                     /locus_tag="ACG842_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0249069.1"
                     /translation="MKEEQTTNKNYRSKTLQDIENEIKKQRAFLAPYTKERIQTTIDS
                     QVKKFHHALLNQRNKEQKKVIMQRSMDILAPLCCLIMTLLAFWLVWGE"
     gene            complement(3858..4772)
                     /locus_tag="ACG842_002586"
     CDS             complement(3858..4772)
                     /locus_tag="ACG842_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HFQ0249070.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATVKTGINCDIDYAKSEMKQIR
                     MLYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPDHQIAIYTHTDKDHIHN
                     HIVINSVNLETGNKFQAHGQAFLNKCYDLNDEICLEHDLTITERGKKPEKRTMSEIKL
                     KEKNEPVWKDEIRFAIDQTMKNKKTRTYNQFCDSLKTFGIHCLNRGKNFTYELIHKKK
                     KVRSNKLGKDYEKETILHELDRRERAYNQQRTNELRRARASIRETAKEVRREQRTLTT
                     EQPEIRPTRRNHDQHVERGADYQQELSF"
     gene            complement(4754..5134)
                     /locus_tag="ACG842_002587"
     CDS             complement(4754..5134)
                     /locus_tag="ACG842_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HFQ0249071.1"
                     /translation="MSEQNQYLASNHSKKYTYRSEPKQISFRVSESEFAKLKQSAEAL
                     QMSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVDPG
                     DAQRLSENLKIMQKELQKIWQQLK"
BASE COUNT         1343 a          952 c          935 g         1993 t
ORIGIN      
        1 ttttattata taacatttta ttatataaca atgattaacg tcataaaaag tagttttgaa
       61 ttcataatcg tttaaataaa attataagat cagttattta aacagctagt agacataatg
      121 tgtatactac caagtaacgc ctgtaccaag ggatccaggc gctgattttg tgaattttta
      181 ttggtctagg ttaaaagctc tttttaaggc ttggttttct gcggttgttt ctttctaatt
      241 ctttgtgaat attgatcggt cattttttaa atacttgtta tttttgtttg tgtttgttat
      301 gctttttaca tatctcatat tcatttatta ttcttttcta aggttaataa taggataatc
      361 tctttcagaa agactgctcg tacgagcagt cttttcttat ttatcaagtt tttctacaaa
      421 tacttcctca ttttgactgt tgtagtaatt aggattgagt tttagttccc cttttttatt
      481 tcctttgttg gccactgata ataacattgg gctaaagctt aatattccta gtaggataga
      541 accgaatagc aagtgttttt ttgtcataag tatttccttc ttcatttcca tagctgccac
      601 cattttttcc aattctttgt ttgttcgtcc attgtttctg ttgcttcaga aagagtcgtt
      661 gaactattct ctgaagactc ttccgtataa gtcaactgca gttgctcctg aagtttttcg
      721 atttgttggt tggcttgcag ggtgagttgt tgttgctggt cgagtagttt ttgtatatga
      781 gaattatttt gctctttgag ggcaagttgt tctcgtaaaa attcattttc ttcacgcaac
      841 tgatctagca acttgtttgc aaccacgttt ttgtttggta tataagcatt tttatcgttg
      901 ttttgttcat gtttatgcaa cttatctagc aacctttgat tgtctaagtt gcgagcaagt
      961 tgcaatactc cagaacgcaa tacaacctta taaccacgct catttttagc tatcatggaa
     1021 gaatctaatt tttggatatg tttcgatatt gcttgcctac tcatcttaaa aagatctgac
     1081 aattccttta ttgtaaaggt ttcttgtggt tgcatactgg ttgcctccta ggttgctact
     1141 ggaataaatt atctaaatca gataataaac tttttttctc tttttccgaa agtgcggact
     1201 ctgtttgttt ttcaagagct tttttctctt tttctagttg tttgatttgt tggaggagtt
     1261 caagttctcg tttcttctgt ttgatttttt cgttaattgt tgtagcgggt tcttcgtatt
     1321 ctttttgttt atcagctaaa gccaatgctt tattttggtc ttctttggtt ggtgcatgtt
     1381 tgttaaagtc gtctgctttt ttgatctcag gtttgaacgt aaattgatag ccaataacag
     1441 gttttccccg tccttttccg tatttctttt tgattgttag tcctttgaag attgcagtta
     1501 gttcttgacg tataggtttt ataactcgtt tatctatatc aggttgctta taacttttag
     1561 gaatatctaa taattcaaga aagtcttctt tggagaaata tgcgaaacct ttggttctgt
     1621 attgctttaa cagacgaaac atagtctttg catagccact ttgtagctcg ttgaattgtt
     1681 gtaaagaata gcgtacccac tcgtctaaat tatttaacaa aggcagtgct ttttcgtaaa
     1741 ttcgtatatc tacgtaagga ttttctacgt ccccgttgat tttaaattct gtaaacatta
     1801 caaacatatc gtaagaaagc ccagactcac ttctacgacc aaagcgtaaa gataagattt
     1861 tttcataagt tgcttttaaa tcttctacaa atcgtacatt tgcagttgct ttgtagttac
     1921 ttaaatcttt tagttggtca aacgtaaaac gaactgtctt atcccctttt tcacgcattc
     1981 tagacacgat ggaaaaaaac aaattcattt caaccggtgt aaattttctt aaaggaattg
     2041 tatttaattc atggtggtat tttacaatct cattagccat ataatcacct caatccaatt
     2101 atgaatgaca ttttttaaat gtcaagacat tcatatttga cgttcaaaca gacaaaatgt
     2161 ccttcataaa cagacaaaat gtccttcata aacagacaaa atgtccttca taaacagaca
     2221 aaatgtcctt cataacattc tgtatccatt ggtagagtaa ggacacagct tacggtaaag
     2281 aggtttaaaa agaggtttaa aaagagatat aaaaagaggg gggctaattt tcccgcctaa
     2341 aaaccacttc ttttttcttt aaacaggttc gcgcattttt caaaactgcc aatccaaaac
     2401 cttgcttcct acggtcgcca aaaaggtgtg atcgctacgc tctcacattg tactgcgcag
     2461 tcgcctaaag gctcctttgc taaaaacatg ttggtaaaga ggcctttgag cggttctgaa
     2521 tgcttatcta agccgtttta agacagtctt ggataaaaat ccttatctag ctttaaagcg
     2581 ccttacaggc ggtcagggca agccctaaca accctaaaat aagcgatact taaaaacacc
     2641 acacaagttt aattttttaa gatataataa cttgtcgcct atccgacagt cttgcgactg
     2701 gaaggaggtg tatctaatgt ttgaattgtt catttgcccc cttcttatag aagtttgtgt
     2761 ctctttattc agttattggt tgaataaccg aaacaagaag taaaatcaat aaaggcgaca
     2821 tcaacctgaa aaaaagcccc agagaatccg acctctctgg ggctttcgtg tatctaatgt
     2881 ttgatcattt gcatgtttag tttaacatga ttgccgatca aattcaattt tgctgttgct
     2941 gatctaacca ttccttgaca ctttttagat aatttaatgc cgtttctttt ttcgttggat
     3001 tttgtatgtt cgttgcttgc tgaagcagtg ctttataacg ttcgtaggta actgattgtt
     3061 gccgttcttt tcgttcctgt tcactagttg aaaaagggat tgtatcactg aataaattat
     3121 aacaagcttc ttctgtctgc gtatctttaa aatattgcac gtcttttttg gttcgctcat
     3181 agttgtcttg aacctctttt tgctgttttt ctggcaagtt ttgataaaga cgagtgagtt
     3241 tttccatgtt tttatccgtc aaacgttttt cttgattcac aaaaatatcg ttcatttcct
     3301 cataacaagt ggtaaactct tccatttttt gtgttaacac ttgctccttt ttctttttat
     3361 cacatgctgc ttcatacttt tgagtgatat cccctaataa aatacttctt gaaatacgtt
     3421 tatttccctc taaaacgccg aataaaccac ttaaaacgat tgataatgta aatataaccc
     3481 ctggcaataa aaacctctta aatcgaattt tagagccttt taatgaatat cttgtttgat
     3541 aagccttata gctaagccaa ataattccta aaatactggc aaaaatgagt agatagggta
     3601 aatagaatgc catttctatt tattctcccc ataccagcca aaaggctaac agtgtcatga
     3661 ttaagcaaca taagggggca agaatatcca ttgatcgctg cataattact tttttctgtt
     3721 ctttatttcg ctggtttaat agcgcatggt ggaatttttt gacttgtgaa tcaattgtag
     3781 tttgtattct ttctttggta taaggtgcaa gaaaagcacg ttgtttcttt atttcatttt
     3841 ctatgtcttg taacgtttta gaacgataat tcttgttggt agtctgctcc tctttcaacg
     3901 tgttggtcat ggtttcttcg agtcggtcta atttccggtt gttcagtcgt aagagttcgt
     3961 tgttctcttc gtacttcttt tgcagtttcg cgaatgctag ctcgagctct tcgtagctcg
     4021 ttggttcgtt gttgattgta ggctcgttct cgtctgtcca gctcatgtaa aatcgtctcc
     4081 ttttcgtaat cctttcctag tttgttggat cgtacttttt tctttttgtg gatcaattcg
     4141 taagtaaaat ttttcccacg attcaagcaa tgaatgccga atgttttaag agaatcacaa
     4201 aattggttat acgttcgagt ttttttgttt ttcattgttt gatcaatcgc aaaccgaatt
     4261 tcgtctttcc aaacaggttc atttttttct tttaatttga tttcagacat ggttcgtttt
     4321 tcgggtttct tccctcgttc agtgattgtt aagtcatgtt caagacaaat ctcgtcattt
     4381 aggtcataac atttgtttaa aaatgcttga ccatgggctt gaaatttgtt cccagtttcc
     4441 agattgacgg agttaatgac aatgtggtta tgaatatggt ctttgtctgt atgtgtgtaa
     4501 atcgcgattt ggtggtcagg ggctattttt ttcgcatact ctgcccctag ttgattggct
     4561 ttttcagcgg taatttctcc tggtcgaaaa gactggatca ataagtgtgc ttgtacacga
     4621 tcgtcttttc catacagcat gcgaatttgt ttcatttcgc ttttggcata atcaatgtca
     4681 cagtttatcc ctgttttgac ggttgctcgt ggttcggcgt aattgatcgc tcggctacaa
     4741 gaagtggttc ggcttacttt aactgttgcc atatcttttg tagctccttt tgcatgattt
     4801 ttaggttttc ggataagcgt tgggcgtcgc caggatcaac gtcgtgttgg tgtgtgttgc
     4861 accacttggc aatttgattg atattcccgc cagctttggc taaatcgcgc gcgattgcct
     4921 gagcaatgtc tggcgccact ttcggtgtca ccagacgcgc gttttgtgcc ttggctttca
     4981 cgaaagccgg cacactcatt tgcaaagctt cagctgactg cttcagcttt gcaaattcgg
     5041 attcgctcac tcgaaaactg atttgttttg gctcggatcg ataggtatat tttttagaat
     5101 gattgctagc caaatattga ttttgttcgc tcataagtcc caccctttca gtacgtccta
     5161 cggacgtatc gttcgcaata ccattgtacc taaaaaggta caataagtaa agctcactaa
     5221 agc
//
LOCUS       DAZKLM010000130         5196 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3764
            SAMEA115706913-rid22582563.denovo.135, whole genome shotgun
            sequence.
ACCESSION   DAZKLM010000130 DAZKLM010000000
VERSION     DAZKLM010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706913
            Sequence Read Archive: ERR13196647
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5196)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5196)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287823.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287823.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,004
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,849
            CDSs (with protein)               :: 2,849
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 41 of 96
            Pseudo Genes (incomplete)         :: 52 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 15 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5196
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706913-rid22582563.denovo.135"
                     /isolate="VRE3764"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            19..368
                     /locus_tag="ACG842_002588"
                     /pseudo
     CDS             19..368
                     /locus_tag="ACG842_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741963.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
     gene            complement(640..930)
                     /locus_tag="ACG842_002589"
     CDS             complement(640..930)
                     /locus_tag="ACG842_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0249072.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
     gene            1332..1568
                     /locus_tag="ACG842_002590"
     CDS             1332..1568
                     /locus_tag="ACG842_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001159903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0249073.1"
                     /translation="MQFLEPKNKNAKSVDWEISEQVRVIVKQYAEYAERTESEAVDEF
                     LLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
     gene            1575..3527
                     /locus_tag="ACG842_002591"
     CDS             1575..3527
                     /locus_tag="ACG842_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HFQ0249074.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
     gene            complement(3599..4096)
                     /gene="dfrG"
                     /locus_tag="ACG842_002592"
     CDS             complement(3599..4096)
                     /gene="dfrG"
                     /locus_tag="ACG842_002592"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HFQ0249075.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
     gene            complement(4423..5115)
                     /locus_tag="ACG842_002593"
     CDS             complement(4423..5115)
                     /locus_tag="ACG842_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HFQ0249076.1"
                     /translation="MKKKFITGGIVVAFLCLIGYTGTRYANEIILWGGETDIDTINEN
                     LTQLDHTLTDKEQAITDLTARLAQKEQTLQDSKADVQEYQTKVNELENQKNQSAAEKQ
                     NLESQLVSKDAQLQSKQNEINVKLDEINQKNDEINHLNADWTARLQEAQAKLSELQNT
                     VNGLNQQIATLTQEKTNVETQLTDTQKKLDEATAENSSLKQYIQKLEKAKDEVEDTAD
                     KSQQIVEEHLRK"
BASE COUNT         1679 a          905 c          862 g         1750 t
ORIGIN      
        1 tatcttttaa atacttaatt gttaaatacc ttatttgatg caaaagaaat ctacgatagt
       61 caacaaaaaa attttcatta tatccagaaa aatttttctt agttaaggac ctatggattg
      121 cagtaatgca gaattcgtcg aataaactgc caaagacata caatcatatt gcttttaaaa
      181 ttgacgaaca agatatagat tcgtttgtct caaaaatcca aatgttaggt ttaactgtcg
      241 aaccaggacg ttctcgtgtg aaaggcgagg cttcttcaat ttacttttac gactatgata
      301 atcacctctt cgagttacat accggtactc tccaggaacg tttaaaatcc tacaattcca
      361 ctacctgaga ctagtaatga ctagtctctt tttatggcct ctgatccccc cattgagcga
      421 atactaatag ggcttcgaat tgatctgttc aatctttaag caatatccct caaatgagtt
      481 tggaaagttt agtgattcat cacaaatact taaatacatg tagttatata tagattgagg
      541 ttcttgtgag cgtatgctcg tgtgggttca aatcccatcg tctgcatcca tctttccaaa
      601 acaagcggct aggacatttt tgtctcagcc gcttgttttc tagtttattc tttcatcttg
      661 tcggaccttt tttcttgggt atagactttt ctcatattct accacaattt tgggtttccc
      721 taacacatga taggatctca caacaaattg ctttctatta ttataataac ctgtaacgac
      781 taacttcatc ccttcgtcta cttcagctag gaagttcaaa ctatgggaag caatcaaaca
      841 attgacccca tttaaactga aacggatcaa cggtctggca ctaacttgca aagtacggat
      901 cttatctact gttccatatt ttttctccat aaccttcacc tcaataccat tatacgaaca
      961 tacattccct ttgtaaagaa gaaactctgt tttatctttt tgactttttg tctattacct
     1021 ttcttaaaat catattggca cttcctgcga agatgaagga catgtactaa aaaaaagagg
     1081 cagcagtata gatttaagta gttcagtttt ggagtacaaa aagtgaaaat gtaaacctaa
     1141 atgagtatca aattgcaact gaatttgata ctcgtttttt attgttaaat caacgtttgt
     1201 tgttaattta caccacaaaa tgtaagttaa aatgtatata aaaatgcgaa cgtgaaataa
     1261 ttattgtagg aatgcaaatt ggtgtgttaa aatggttaca atttactaag caaaaagggg
     1321 tgcattttcc tatgcaattt cttgagccaa aaaataagaa cgctaagtct gtggattggg
     1381 aaatatcaga gcaagtaagg gtgatagtta agcaatacgc tgaatatgca gagcgtactg
     1441 aaagcgaagc agtggatgaa ttcttattga acattttaga cgataagaag tttattgaat
     1501 ggattgccaa caagcgaagt aataagcgga ttgttgaaaa aatgggtatc aaagatagag
     1561 tgggttgata gacaatgacc aagcttaaaa gattagccac gaaggaagat gaaattgtag
     1621 atgttaaaat tccgatttca aacgaagaat taaaagatag ggctaaacaa tatgatttat
     1681 taactccaaa acgatttgca acaaggtata acaaaatgtt gttcttgcca acgtctttta
     1741 aatggaacgg ttctgaatat cccattcaat ataattactg catcaatccg ttttgttgta
     1801 attttgggaa agaacagcac aaattcaaag atgtaaaagg gaaaccaagt cgatataaaa
     1861 tgactggtag ttccaaagat aaaggtcata aaggtatgta ctgcaatgac aatcctattg
     1921 gcagaggtgt atcgcaaaac tgtacagtaa cccctttatc taattggtct gttgttgaag
     1981 aaattaaacg ccttatagaa atcaatagca tccaagatgt tgagccagat tatcaatttc
     2041 ataaagaagg ctgctcggaa gaagaatcaa ccccattcaa tgagccgaag caattttaca
     2101 aaagggggaa aagtaggggc aaatctcaac gctatcaatg taaggcgtgt aagaagttca
     2161 ctaatgttct accaaaaaga gaagaaacca ctacttatca ccaacaaaag aacaccattc
     2221 ttccaatgtt cgctaagatg gttgttggaa gggtttcagt cagtcgtact tgtgatattt
     2281 taggaattgg agttggaacc tactatcata agctggagtg gctgtatagg cgttgtttgg
     2341 agtttttaga acgatatgag acacaacccc ttcaaacaaa gaaatttaac gagatgtggc
     2401 ttaatacgga caagatgcac tactacctaa ataacgtccg taagaaagga caaggttcaa
     2461 agaaatatac ggggtttgaa gaccttaata tgcaaacgta tatagttgta tctgctgagg
     2521 tcttatcaag atatgtgttc cgttctgatg ttgcctatga ttggaatatt tcaatggatg
     2581 aattaaatga agatacaaga aaattcaaag aagaccatct caatactttt agtcgtaaaa
     2641 atgataggtt agattggtct tattatccac aagaaccatc cgctaacgat agcgaaaata
     2701 ggaatgctta tcttcacgaa ttagggaaaa ttactaatag gagtagattt gtcgatggtt
     2761 taaatgttga tgctccttat actacaactg ctcactattg gttaattaaa caaatggtta
     2821 atgccgacga atggcgaatg attagtgatg atgacttctc tattagaaac gccttttata
     2881 gagtattcac gaaagaatta aggttgagcg atgcacatca ttttatttgc caagttaaca
     2941 agacgaaaag cagaaaacaa tgcttaaaag aattcggaca agcaaaagca gaactgctcg
     3001 attggggaga tatcagagga ttcaaaacaa agtttctacg gactttggca tctcattatc
     3061 taactgaatt actaacaagt catcaattcc acgaagaggc tattagtaaa gatggagaaa
     3121 gatatcgcaa atatgccgat aaccctatca aacatccatt agccactaag gataaaggat
     3181 tttattcagt tgattgtaga acggatttat ctgctctgga accaaacgaa atcgcaaaga
     3241 tgctattaaa tgttaatgac cattcgacca atagctttat tcaacagata agacgttata
     3301 tatcatctct tgaaagacca ttaacaacag ctcgtggtga taagaagagc tatatttacg
     3361 ctaacttcaa tccgaagtat gctcaatttg cgattacaat tctaagaact tactacaact
     3421 tttgtcgccc ttttaaatca gcggataaaa aggttttgac acctgctcaa agattaggga
     3481 ttacagataa acaatttgat tggaaagata taatttattt caaatgacaa aaagagcttg
     3541 gagtaatccc aagctcttgt gctttatctt tactgttctt cttcaattaa cgcacccgtt
     3601 aactcaataa gttttttctt tcatatacat gaaaatagta gttatacgga tttttatcat
     3661 tctttatccc tttttgggca aatacctcat tccattcctc ataattcact tctggaaaaa
     3721 aagtatctcc ttcgaattca tgatgtattt ttgtgatgta cattttctca acataaggga
     3781 aaaacaaatt ataaatctgt tctcctccga aaataaaaat ttcttcttcg tttttacata
     3841 actcaaaaac atcttctatt gaatgaacaa tttcacaacc attaaaggta aaccccttat
     3901 ctctcgtcag aataatattt cttctgtcag gtaaggctct tccgattgat tcaaggttct
     3961 tcctacctaa tattatcgga tgtccctttg tagtattttt aacatattcc cagtccttgg
     4021 gaatcctcca aggaatgtca ttctctttgc caatcactct attcttatcc atcgcagcaa
     4081 tcaaagaaac tttcatttat aacacagcct ttttaactaa tttaccatct aatttcttat
     4141 ttctatcaaa aaaccttttc tcattatcct gcccgttagt tgaataagca attttttcta
     4201 taagttatat tatcataata ccacaaaatc acaaattttc attcaattat cattttcaat
     4261 tgcattttat aaatcatttt gtatgtaatt tggtatgtaa gttaaaatga aaaaagtagt
     4321 ccattttgaa gtacattttg tcattttgta ctccaaaact gaactactta gtatagatta
     4381 ctgttgcttc tttactaatt taggtttact tgttaagaaa gattatttac gcaaatgttc
     4441 ttcaacaatt tgctgagatt tatcagctgt atcttccact tcatcttttg ctttttctaa
     4501 cttttgaata tattgtttca agcttgagtt ttcagcagtt gcttcgtcta atttcttttg
     4561 tgtatctgtt aactgagttt ctacatttgt tttttcttgt gtcagtgttg cgatttgttg
     4621 attcaaacca tttacagtat tttgtaattc tgaaagtttt gcttgtgctt cttgtagtct
     4681 tgctgtccaa tctgcattca gatgattgat ttcatcattt ttttggttga tctcatctag
     4741 tttgacattg atttcatttt gtttgctttg tagttgtgca tccttgctga ctaattgact
     4801 ttctaagttt tgtttttctg ctgccgattg atttttttgg ttttctaatt catttacttt
     4861 tgtttggtat tcttggacat ctgcctttga atcttgcaat gtctgttctt tttgtgctag
     4921 tcgagcggta agatctgtaa tcgcttgctc tttatctgtc aatgtatgat caagctgtgt
     4981 cagattctca ttgattgtat caatatctgt ctctccaccc cacaaaataa tttcatttgc
     5041 gtaccgcgtg cctgtgtagc caattagaca tagaaatgca acgacaatcc caccagtaat
     5101 aaatttcttt ttcatttgca tagctcctta atccaatact tttacatgct aaaagtaagt
     5161 gatctcaaac acaatgtcaa gcgtttttaa attaca
//
LOCUS       DAZKLM010000131         5189 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3764
            SAMEA115706913-rid22582563.denovo.136, whole genome shotgun
            sequence.
ACCESSION   DAZKLM010000131 DAZKLM010000000
VERSION     DAZKLM010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706913
            Sequence Read Archive: ERR13196647
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5189)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5189)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287823.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287823.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,004
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,849
            CDSs (with protein)               :: 2,849
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 41 of 96
            Pseudo Genes (incomplete)         :: 52 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 15 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5189
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706913-rid22582563.denovo.136"
                     /isolate="VRE3764"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            137..748
                     /locus_tag="ACG842_002594"
     CDS             137..748
                     /locus_tag="ACG842_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HFQ0249077.1"
                     /translation="MYFILPTLYSLLCVMAPCIIYMVFFCKKNTLKHNILILIFMLYC
                     WKVFELTGTGGLTDIFYRPDTGRKFSLVQGSVNLVPFSGLNISFILNIVMCVPLGFLL
                     PIIWKSYRKIVPVFLAGALFSLIIEVSQIITTRATDIDDLIANTFGAVIGFILWAVLS
                     RVVSLKDKSSTVDYLEPYIYILIGFTGKFLLYFPFWFAFEVLS"
     gene            821..1750
                     /locus_tag="ACG842_002595"
     CDS             821..1750
                     /locus_tag="ACG842_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HFQ0249078.1"
                     /translation="MKRFLKWGLVLLASLIVILMCTAVVISYVNRPYARAVYDRIKRI
                     LDKDFYDHLYLGSSFEIINLDTMDIHSYMKKDQEELPASLSKLFTIDYALSVLSLDEQ
                     ITISPTALDMQKADSSTAHLQEGDILSVYDIICAMVLPSGNDAAYSLAIYTGGKLDPD
                     AKNDEERYQSFMKNLDRYLSEQGYKHTHFTDAAGYDSNATTSAEDLTKVAAKLLKNDK
                     FAEIVAKETYRAKSIKGNVYNWNTTNQFMQQDNRYFTEGVKGVKTGSFDGRYNLCVYY
                     TSSSDNHYLLVSFGSESDSRRYVDVQNLLHNLP"
     gene            1887..2645
                     /locus_tag="ACG842_002596"
                     /pseudo
     CDS             1887..2645
                     /locus_tag="ACG842_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033645.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VirD4-like conjugal transfer protein, CD1115
                     family"
     gene            complement(2693..3895)
                     /locus_tag="ACG842_002597"
     CDS             complement(2693..3895)
                     /locus_tag="ACG842_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HFQ0249079.1"
                     /translation="MHRKEDFHMNDFTTEILKTLANKGDLNELFRVHLEKAVNTLLKT
                     ELTAFLDYEKYDRIGFNTGNSRNGSYDRTVKTEYGELHLQIPRDRNGEFKQQTVPAYR
                     RTNDTLEETVIHLFRKGITMSEIADLIEKMYGHHYTPQTMSNMTKSFTEEVTSFKERE
                     LHDRYAAIYMDATYIPLKRKTVAKEAIHIAVGIRPDGSKEVLSYAIAPTESITIWEEI
                     LIDLQERGVKNVLLFITDGLKGMAGAVQRFYPKARFQHCCVHVSRNISHKVRVDDRKE
                     VCDDFKMVYQASSKKAALEARGAFAEKWKTSYPKMVESILSNDYLLTFYDFPLAIRKT
                     IYSTNLIESFNKQIKKYSHRKEQFQNEESLERFLVSIFDTYNQKFLNRSHKGFQQVTD
                     TLASMFTE"
     gene            3975..4499
                     /locus_tag="ACG842_002598"
                     /pseudo
     CDS             3975..4499
                     /locus_tag="ACG842_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296840.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(4706..>5189)
                     /locus_tag="ACG842_002599"
     CDS             complement(4706..>5189)
                     /locus_tag="ACG842_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFQ0249080.1"
                     /translation="KEAIDQIKTAVLYPHNGLPILLMGVSGSGKTFLAEKIYEYAKEQ
                     QVIESNAPFIAYNCAQYFNFSINKIVENLTILDAMKVLRLVQKAVKELEKLMGIEFSN
                     NQLFLLYMHCCSMIERILRKESVDEQADIKEYIQKEGHNYEISKYPAYKTKFESTVIK
                     "
BASE COUNT         1639 a          879 c         1003 g         1668 t
ORIGIN      
        1 cctctaggaa aaaattaact agcaccacgg gttatgatat aataaaatgt attataggca
       61 tttttttaca agaaatagaa gcttgaagtt tatatgaagt tataaaattt aatttatatt
      121 ggaaggagga aaataaatgt attttatact acctacactt tatagccttt tatgtgtaat
      181 ggcaccatgt attatttata tggtattttt ttgcaaaaaa aataccctga aacacaatat
      241 cctgattttg atttttatgc tgtactgttg gaaagttttt gaattgactg gtacaggtgg
      301 actaacagat attttttata gacctgatac aggtaggaaa ttttctctag tacagggcag
      361 tgttaatctt gtaccattca gtggactaaa cattagcttt atattaaata tagtaatgtg
      421 tgtgccctta ggattcttac taccaatcat atggaaaagt tataggaaaa tagttcctgt
      481 tttcttggct ggggctctat tttcactaat tattgaagtc tcacaaatta taactacaag
      541 agcaacagat attgatgatt taatagcaaa tacctttggt gcggttatag gctttatact
      601 gtgggctgtt ttaagtagag tggtgtcctt gaaggacaag tcatctacag tagattattt
      661 agaaccttat atttatatat tgattggttt taccggaaaa ttcttactgt attttccttt
      721 ctggtttgct tttgaagtat tatcctgata attccataga aaataatgag tttgagttga
      781 gaaagaatat tcttgtacac aactctggaa aggaggaagt atgaaaagat tcttaaaatg
      841 ggggcttgtt cttttagcgt ctttgattgt gattctaatg tgtacagcgg ttgttatttc
      901 ttatgtgaat cggccgtatg caagagctgt ttatgatagg attaaacgca ttcttgacaa
      961 agatttttat gaccatctat atctaggttc ttcatttgaa ataataaacc ttgatacaat
     1021 ggatattcat agctatatga aaaaagacca agaagaatta cccgcctcat tgagcaaatt
     1081 atttacgatt gattatgcgc ttagtgtcct aagtttagat gaacagatta caattagtcc
     1141 tactgcactt gacatgcaga aagctgactc atctactgca catcttcaag aaggagatat
     1201 tttatctgtt tatgacatta tctgtgctat ggtcttaccc tcaggaaatg atgcagctta
     1261 tagtttggca atttatacag gtggaaaact tgatcctgat gcaaagaatg atgaagaacg
     1321 ctatcaaagc ttcatgaaaa acttagatcg ttatctaagt gagcaaggct ataaacatac
     1381 tcattttact gacgctgctg gctatgactc caatgctaca acatcagcgg aagatttgac
     1441 aaaagtagct gcaaagctac taaagaatga taaatttgca gaaatagttg ccaaggaaac
     1501 ttatagagca aaatcaatta agggaaatgt ttataactgg aatactacga atcaatttat
     1561 gcaacaagat aatagatact ttactgaagg tgtaaaaggt gtaaaaactg gatcgtttga
     1621 tgggagatac aatctatgtg tgtattatac atctagttct gataaccact atttacttgt
     1681 aagttttggt tcagagagtg atagtaggcg ttatgtagat gttcaaaatc tattgcataa
     1741 tctgccttga aatttcgtgc ctttaagcgc gtatctaaag tttctttgaa aagtttgaga
     1801 atgaagaaag tgagcagtcg aaaaacggtt gctcactttt ttgccctaaa atcgaaaaaa
     1861 tataaaaaag tcctttacaa cacgtctcag gaaaaactgc taagtcaatt ctaatatctt
     1921 gtggagcaag acttgcacct tttgatataa gagagcttag agaactaatg agcgaagatg
     1981 aactagaact cgataaaatt ggagatagaa aaactgcctt gtttgtaata atatcagata
     2041 cagatgatac atttaatttt gtagtttcaa taatgtattc tcaattattt aatctactat
     2101 gtgataaggc agatgatgtg tatggtggaa gacttccagt tcatgttagg tgtctacttg
     2161 atgagtttgc aaatatcggt ttgattccta aatttgaaaa actaatagcg acaattcgtt
     2221 caagagaaat atcggcaagt ataatactgc aagcacaatc tcaattaaaa gcaatttata
     2281 aagaccatgc agatacaata gttggtaact gtgactctac actttttcta ggaggaaaag
     2341 aaaaaacaac agtaaaagaa ttatcagaaa ccttaggaaa agaaaccata gacctatata
     2401 acacatcaga aacaagatca aatcaaaaat cttttggtct aaattatcaa aagactggca
     2461 aggaacttat gagtcaagat gaaataactg taatggatgg tggtaagtgt atttaccagc
     2521 taagaggagc aagacctttt ctatctgata aatttgatat tacaaagcat aagaattaca
     2581 aattgttgga agattatgat aagaaaaact tgtttgatgt agaagagtat ttaaaacaaa
     2641 gatagcgtaa aatattttgt gtaaataact cgtccttctg caaaataatg ggttactcag
     2701 taaacattga agctaatgta tccgtcactt gttggaagcc tttatggctt ctatttaaga
     2761 atttttgatt gtatgtatca aagatggata ctaggaaacg ctctagtgat tcttcatttt
     2821 gaaactgctc ttttctgtgg ctgtatttct tgatttgctt attaaaggat tcaatcaagt
     2881 tcgtagagta aatagtcttg cgtatggcta atggaaaatc atagaaagtg agcaggtaat
     2941 cgttcgaaag gatcgattcc accatttttg gataactggt tttccatttt tccgcaaaag
     3001 caccacgtgc ttccaatgcc gcctttttag atgacgcttg atacaccatt ttaaaatcat
     3061 cacagacttc cttacgatca tcgacacgca ctttgtgact gatattacgg gaaacgtgca
     3121 cacagcaatg ttgaaaacgg gctttggggt agaaccgctg cacggcccct gccatcccct
     3181 ttaagccatc cgtgatgaaa aggaggacgt ttttcacgcc acgttcctgg aggtctatta
     3241 aaatctcttc ccaaatcgtg atagattcag ttggtgcaat cgcatagctc aacacttcct
     3301 ttgaaccgtc cgggcgaatg ccaactgcga tatgaatggc ttccttggcg acggttttcc
     3361 gctttaaagg gatataggtt gcgtccatat aaatagcggc ataacggtca tgaagctcac
     3421 gttccttaaa tgaagtgact tcttccgtaa atgatttagt catattggac atggtttggg
     3481 gcgtgtagtg atgcccatac attttctcaa tcaagtctgc gatttccgac atggtaatac
     3541 cttttcggaa gaggtgaatg acggtctcct ctaaggtatc attcgtccgt ctataagcag
     3601 gaacagtctg ttgcttgaac tcgccgttgc ggtcgcgcgg aatctggaga tgaagttccc
     3661 cgtactcggt cttgaccgta cggtcatagg agccgttacg agaattaccc gtgttaaaac
     3721 caatgcgatc gtacttttcg taatcgagga aagccgttaa ctccgttttg agaagcgtat
     3781 tgacagcttt ttccaaatgg acacggaata attcattcaa atcgccttta ttcgctagag
     3841 tctttagaat ttctgtagta aaatcgttca tatggaagtc ctcttttctg tgaatgtgtt
     3901 gtggtaactc tattctactg aagggacttc cttttttcta tttacacaaa atattttaca
     3961 ctctcggatt gaaagaacgc ggtctttcag gagtaaagat ggtcatctcg gatgcgcata
     4021 tagggctcac aaaagctgtg aaagaatacc ttctttgtgc gagctagcag agatgccaag
     4081 cacatctcct tcgcaacatt tccgacaaat tctccaagaa aatctcaagc gatatcaaag
     4141 aagactttaa agccatcttc aaaacggcaa gcatcgagct ggctggagaa cgtaaacaac
     4201 ttattatgga taaatatggg gaagacaaga aagtcgcaac tgcctgtaaa atattggatg
     4261 agggcttcga agatgccatg caagtaatgg ttttaccaga aaatatccgt caacgattga
     4321 gaacaacgaa tgttcttgaa agactaaatg aagaagtacg ccgtcgtgaa cgtgttgtcc
     4381 gaattttccc aaattgtgat tctgttattc gtatcatcgg tgctttattg atggaaaagg
     4441 atgacgaatg gacttctgca cctagaaaat atcttgaatt tgatcgtaac gagatttagc
     4501 acattaagcc gccaatagta taatatactg gtgagaagta tcaagagacg cttcgctttt
     4561 tgctcttgac acttcttacc agcatattgg accgtagcta ggctcaacaa ggcaaaacat
     4621 cgtactttga gatttccttt ttacacaaga ttttggactt gacacagccc ctatggttct
     4681 taccttgttt atgcttattt aaatcttact ttattacagt actttcaaac ttcgttttgt
     4741 aagccgggta ttttgatatt tcataattat gtccctcttt ttgaatatat tctttgatat
     4801 ctgcttgttc atctacactt tctttgcgta aaatacgttc gatcattgag cagcagtgca
     4861 tgtaaagcaa gaacaattga ttgtttgaaa attcgatccc cattagtttt tcgagttctt
     4921 tgacggcctt ttgtaccaat ctcaaaacct tcattgcatc aaggatcgtt aaattttcta
     4981 cgatcttgtt aatcgagaaa ttaaagtatt gagcacaatt ataggcgata aatggagcat
     5041 ttgattctat cacctgttgt tccttggcgt attcataaat tttttctgcc aaaaaggttt
     5101 ttcctgaacc gcttacaccc atcaataaaa tcggtaatcc gttatgagga tacaagacag
     5161 ccgtcttgat ttgatcgatc gcttccttt
//
LOCUS       DAZKLM010000132         5137 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3764
            SAMEA115706913-rid22582563.denovo.137, whole genome shotgun
            sequence.
ACCESSION   DAZKLM010000132 DAZKLM010000000
VERSION     DAZKLM010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706913
            Sequence Read Archive: ERR13196647
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5137)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5137)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287823.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287823.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,004
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,849
            CDSs (with protein)               :: 2,849
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 41 of 96
            Pseudo Genes (incomplete)         :: 52 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 15 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5137
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706913-rid22582563.denovo.137"
                     /isolate="VRE3764"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..447)
                     /locus_tag="ACG842_002600"
     CDS             complement(<1..447)
                     /locus_tag="ACG842_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0249081.1"
                     /translation="MNYEFFLSKEDHRSYSLLKYLEASSNLSESISNVQEELSLSTFL
                     LKKTIDKLKHDLQKFDLEKNLRVTVSELDICLEINGKCSSKALLSRYITDSLSMKMVL
                     AFFQEKYISVENFAKQHHVSYSVAYKVLQRLKRNLKKYQIFFEKRKL"
     gene            complement(754..1851)
                     /locus_tag="ACG842_002601"
     CDS             complement(754..1851)
                     /locus_tag="ACG842_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HFQ0249082.1"
                     /translation="MIKGAIFLRPKQQLFITLLVILGALFNNISVSASAFNFSVTPIT
                     SENQIDKRKTYFDLQLDPDQEVEVKAELRNDTEKEVKIDISVNSATTNSNVMVEYGKN
                     EIEKDMSLIFDLVDYVSYPQIVTLKPKSVQTVAFHVRMPNERFDGVLAGGITFQEIQT
                     EKDQTETKDQSLSIENEYTYIIALLMQQTLNEVAPNLTLHEVKPDQINARNVILANVQ
                     NDQKTYINQVVIETKITKKGHSEVLYQEEKEGLQIAPNTNFSFPTALNGQPLTPGEYH
                     LTMTVLGNENENGKFSRKKGNTTINYTNQWVFEKDFTIDGKVAKELNTTDVTIKKDNN
                     WIYILVASLFLLFILLCILLFVLRKKEEKEK"
     gene            complement(1959..3986)
                     /locus_tag="ACG842_002602"
     CDS             complement(1959..3986)
                     /locus_tag="ACG842_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucBP domain-containing protein"
                     /protein_id="HFQ0249083.1"
                     /translation="MKKRRIVLILSSLLVTNIFPIIGYSLELNQLEHGNRRDEESKLT
                     STFDSLIESNQTQESSEDLSISSSGETTDNVEAEGSDKQGLEDAPISAVELPDDPNII
                     SIDKIFQTPIGTSTSILENGKLLQLNPAERSQRGAIWSNKKISLLSDFTFKSYLYLGN
                     QRDRAGDGMTFTLTGDPRMASNPSQVIGSPGMGIGSYSTRSGQPYIANALSIEFDTYK
                     NSGSSDRMDREITADNRGHGHVAFVTPKPNNNNYSGEHTGVTVAPTFLSNGTWRLLII
                     KWDAEQRSLTYDLEGVGRSSRIIPDLIRQFGSTEVNWGFTSSTGTFFQENAVAVTRLP
                     SNVTSTADVKVNDSDYGAIAEAGGNDKITIRNTLKVEGDFLEYKKMQTSIDLPQELTI
                     PEHILINGNQIDAKDILITGNRVTLNLTDYFIAGDTLIIELETTLQEVIPEKVLTMHF
                     EYLENGNLIEQSNEINLKIAKLKEIDIHVYYKDKDSQIDIAEMKTITGKIGEKYKETP
                     IDIEGYVFDSDSANTEGIFSENTEDIYFYYRKGKLYLSTAPQYVNFGKHKISSRPLSE
                     FGQFSGGLKIIDERAFGSWKLQLKQEKVLTNGDFELPEALSFVTSENTTVIGTSAVTI
                     FESDQKGESDLSSLLDSTAHRGIRINIPVENQRIGTFEGKLSWILADVPGN"
     gene            complement(4131..4859)
                     /locus_tag="ACG842_002603"
     CDS             complement(4131..4859)
                     /locus_tag="ACG842_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HFQ0249084.1"
                     /translation="MKKEIVLTAAMMLFSMVASTTFVSATEVYPKEYNTEGTITFEAG
                     DEGVTPPVDPENPDPNKPVDPSDPPSPGTGGALSIDYGSKFKFGTQKISTADKTYYAA
                     ADVMNDGSRKPTYVQVTDRRSTLSGWKLSVSQPEQFKTASGDELVGAQLKFTKGQAVS
                     LVDPTYTPQTVNSELTLTPGGNNTLAINAKSGTGVGTWVYRFGANENENQDAVQLSVP
                     GKSVKLAQQYSTKLVWTLEDTPNN"
BASE COUNT         1456 a         1040 c          813 g         1828 t
ORIGIN      
        1 caactttctc ttttcaaaaa aaatttgata tttttttaga tttcttttta gtctttgtaa
       61 tactttataa gccacagaat aactcacgtg atgctgttta gcaaaatttt ctactgagat
      121 atatttttcc tgaaagaaag ccaataccat tttcatagat aaagaatccg ttatataacg
      181 agataacaga gctttgctag aacattttcc attaatttcc agacatatat ccaattcact
      241 gacggttact cgcaaatttt tctctagatc gaacttttgt aaatcatgtt tcagcttatc
      301 gatcgttttt ttcaagagaa atgtcgatag cgacaactct tcttgaacat tcgatattga
      361 ctctgataaa tttgagcttg cttctaaata tttaagtaat gaataacttc tgtgatcttc
      421 tttgcttaaa aaaaactcat aattcattag tcatcagctc tttctacgta tattactttc
      481 catgcattca ccaactagct ggggataaaa ctgatttcct ggatccgagt cactgttgtc
      541 agcgattctt gtttgtataa tatacatcgt cctgtctttt ttttaacatt ttggagagcg
      601 ttttatttgg cctttctcgc gatcaaacct gttattagaa ttatatcatt tactagtatg
      661 catttttatt aataaaatgg tatattttat attgttataa aaagagccca accatcctac
      721 aataataaaa atttactatt tgttatctta accttacttt tctttctctt ctttttttct
      781 caaaacaaac aaaagtatgc ataataaaat aaacaataag aaaagtgaag ctactaagat
      841 ataaatccaa ttattgtctt ttttaatcgt aacatccgtc gtgttcaatt ccttggctac
      901 ttttccgtct attgtaaaat ctttttcaaa tacccattga ttggtatagt tgatcgtagt
      961 atttcctttt tttctcgaaa attttccatt ttcattttca tttcctaaaa cagtcattgt
     1021 aaggtgatat tcacctgggg ttagcggttg accatttaaa gcagttggaa aagaaaaatt
     1081 tgtattcggc gcaatctgca atccttcttt ttcctcttga tataaaactt cagaatgtcc
     1141 tttttttgta atttttgttt caataactac ttgattgata taggtttttt gatcattttg
     1201 aacgtttgct aagatgacat ttcttgcatt gatttgatct ggtttaactt catgaagagt
     1261 aaggttcgga gcaacttcgt tcagtgtctg ttgcatcaac aaagcaataa tataggtgta
     1321 ttcattctca atcgataagc tttgatcctt agtttctgtt tggtcctttt ccgtctggat
     1381 ttcttggaat gtgatcccac cagccaatac accatcaaat cgctcattcg gcatcctgac
     1441 atgaaaagca acggtttgga cacttttagg ttttagcgtc actatctgtg gatagctcac
     1501 ataatctacc agatcaaaga tcaggctcat gtctttttca atctcgtttt ttccgtattc
     1561 aaccatcaca tttgaattgg tggttgcgct attcacagaa atatcgattt tcacttcttt
     1621 ttctgtatca tttctcaatt ccgcttttac ttctacttcc tgatccggat caagttgaag
     1681 atcgaagtaa gtttttctct tgtcgatttg attctctgaa gttatcggcg tcactgaaaa
     1741 attaaatgca ctagctgaca ctgatatatt gttaaaaagt gcccctaaaa taactaggag
     1801 tgttataaat aattgttgtt ttggtctcaa gaaaatcgct cctttaatca tatgtaagag
     1861 aagactataa caaaatcttg tctctgtggg acacaagagc ctatggtacc tgtgcccttc
     1921 ttgaaactca ataattttgt tatattcttc tcttcctgtt aatttcctgg gacatctgca
     1981 agtatccaag atagtttccc ctcaaaggtt ccgattcttt ggttttctac aggaatattt
     2041 attctgattc cacgatgagc tgtagaatct aaaagagagg acaaatcgct ttcacccttt
     2101 tgatcacttt cgaaaatcgt aacagcggat gtccctataa ccgttgtatt ttcagaggta
     2161 acaaaggaaa gagcttctgg aagctcaaaa tctccatttg taagaacttt ctcttgtttc
     2221 aattgtaatt tccaacttcc aaaagcccgt tcatctataa ttttcaaccc acctgaaaac
     2281 tgtccgaatt cacttaatgg tctactggat attttatgtt ttccaaaatt gacatattga
     2341 ggagcagttg agagataaag tttgcctttt ctgtaataaa aatagatatc ctctgtattt
     2401 tcggagaaga tgccttctgt attcgctgaa tccgagtcaa aaacataccc ttcaatatca
     2461 ataggagttt ctttatactt ctctcctatt ttcccagtga ttgttttcat ttcagcaata
     2521 tcaatctgac tgtctttatc tttataataa acatggatat ctatctcctt cagctttgcg
     2581 atctttaggt ttatttcatt cgattgctca atcaaattcc cgttttcaag gtattcaaaa
     2641 tgcatagtga gtactttttc aggaattact tcctgcaaag ttgtctccaa ttcgattatt
     2701 aacgtatctc cagcaataaa ataatctgtc agatttagcg tgactcgatt tccggttatc
     2761 aagatatctt tggcatcaat ttgatttcca ttgatcaaga tatgctcggg aatagttaat
     2821 tcttgtggga ggtcgatact agtctgcatt ttcttatatt caagaaaatc cccctcgacc
     2881 ttcaatgtat ttctgatagt tatcttatcg tttcctccag cttcagcgat ggccccataa
     2941 tcactatcgt tgacttttac atcagctgtt gatgtgacat tcgatggaag tcgcgtgaca
     3001 gcgacagcat tttcttgaaa aaaagtacct gttgaagaag taaaccccca atttacttct
     3061 gtagaaccaa actgcctaat gagatcaggt ataatccgag aagagcgacc gacaccttcc
     3121 aaatcataag taagacttct ttgctccgcg tcccatttaa ttattaacaa gcgccatgtg
     3181 ccattagaaa gaaatgtagg agctaccgtc acgccagtat gctctccaga ataattatta
     3241 ttatttggtt taggcgtgac aaaagcgaca tgtccatgcc ctctattatc agcagttatc
     3301 tctcgatcca ttcgatcaga agaaccacta tttttatacg tatcaaactc aatcgagagc
     3361 gcattcgcaa tgtaaggttg gccagaacga gtagaatatg agccaattcc catacctggc
     3421 gatccaataa cttgactggg attactagcc attctaggat cgccagtcaa tgtaaaggtc
     3481 atgccatctc ccgctctatc tcgttgattt cctaagtata aataagattt aaatgtaaaa
     3541 tccgagagta aactaatctt cttatttgac caaatagctc ctctttgaga cctttctgct
     3601 gggttcaact gcagtaactt cccattttcc aatatggacg tgctagtacc gataggtgtt
     3661 tgaaaaattt tatcgataga aataatattg ggatcgtcag gcagctcaac cgcagaaatt
     3721 ggtgcatctt ctagtccttg tttgtccgaa ccctcagctt ctacgttgtc agtggtctct
     3781 ccactactcg aaatacttaa atcctcactg ctttcttgtg tctggttgga ttctattaaa
     3841 ctgtcgaatg tcgaagtgag tttgctttct tcgtctcttc tatttccatg ctccaactga
     3901 ttcaattcca aagagtaccc tatgatcgga aagatattag tcactaagag actgcttaaa
     3961 atgagcacga tcctcctctt tttcaatata tcttttcctc ctgatcgtta aattcctaga
     4021 tgaactattc gtatccaaac aaatttagcc tagcctttga caatttttgc tcgattacct
     4081 cgcaagacaa tatgactacg tatttatttt taacagttct tttcagtaaa ctagttgtta
     4141 ggtgtatctt ctaatgtcca aactaattta gtggaatatt gttgcgctaa cttgacagat
     4201 ttaccgggta ccgaaagctg taccgcatct tgattttcat tttcatttgc accaaatcga
     4261 tagacccaag tccccacacc tgttcctgat ttagcattaa ttgctaacgt attgtttcct
     4321 cccggtgtca acgttaattc agaattcacc gtttgcggcg tatatgttgg atcaaccaaa
     4381 gaaacagcct gccctttcgt aaatttcagc tgtgctccaa ctagttcatc cccactggca
     4441 gttttaaact gttctggttg agagacactt aatttccacc cagataaggt acttctccta
     4501 tctgtcactt ggacatatgt cggttttcta gacccatcgt tcatcacatc agcagctgca
     4561 taatacgttt tatccgcagt agaaattttt tgagtcccaa atttgaattt tgatccataa
     4621 tcaatcgata atgcccctcc tgttccaggg cttggaggat ctgatggatc gacaggttta
     4681 ttgggatcag gattctctgg atcaacagga ggagttacac cctcatctcc tgcttcaaat
     4741 gtaatcgttc cttctgtgtt atattccttt gggtatactt cagttgcaga aacaaaagtc
     4801 gtacttgcta ccatgctgaa taacatcatt gctgctgtta atacgatttc ttttttcatc
     4861 attgacattc ctctttcttt atttactaaa catatcagtc gattggatta atccatagca
     4921 aaattctgtt ttacgatact tatgaaataa aagaacatag acctatattt ggtcctatgt
     4981 atcgattagt tataaatgat tatcagcccg atttgatttc tagtttttat tgcaacttga
     5041 tgcaaacaca atctagaaat acagctgtca tagcctttaa aataattcgt aagtccccct
     5101 cctatctatc aaaatgtgac ttgacgagag tgtaaaa
//
LOCUS       DAZKLM010000133         4931 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3764
            SAMEA115706913-rid22582563.denovo.138, whole genome shotgun
            sequence.
ACCESSION   DAZKLM010000133 DAZKLM010000000
VERSION     DAZKLM010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706913
            Sequence Read Archive: ERR13196647
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4931)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4931)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287823.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287823.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,004
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,849
            CDSs (with protein)               :: 2,849
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 41 of 96
            Pseudo Genes (incomplete)         :: 52 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 15 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4931
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706913-rid22582563.denovo.138"
                     /isolate="VRE3764"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            83..970
                     /locus_tag="ACG842_002604"
     CDS             83..970
                     /locus_tag="ACG842_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HFQ0249085.1"
                     /translation="MQELKTRSARHHRPKRKMVINKKAIAPLIGTSLLVGPMMVTAVP
                     QHVHADEQGSQTVADPQAFIDQIGWSASEVAASNDLYASVMIAQALLESAYGTSGLAS
                     APNYNLFGVKGSYNGQVVYMATKEYVNGQWVTMNEPFRKYSSYWESFQDHANVLRSTS
                     FSTGDYHYAGVWKSNTTSFYDATAYLTGRYATDPNYGAKLNSLINTYNLTRFDTPSTG
                     SATTTTSTTTTTTTTTSTSSSSTTDVNTNNGQTYTVVSGDTLWDIAQKFGMSVDDLMA
                     KNGMSMSSYSLLVGQELNV"
     gene            1110..2036
                     /gene="cysK"
                     /locus_tag="ACG842_002605"
     CDS             1110..2036
                     /gene="cysK"
                     /locus_tag="ACG842_002605"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HFQ0249086.1"
                     /translation="MTAIYNSVTELIGKTPIVKLNKIVPEDSADVFVKLEFFNPGGSV
                     KDRIALSMIEKAEHDGLLKPGDTIIEPTSGNTGIGLSMVGVAKGYKVIIVMPETMSIE
                     RRLLMKGYGAELILTPGADGISGSIREAERLAKENGYFLPLQFENEANPLVHEKTTGP
                     EIHQAFGVNGLDAFVAGIGTGGTITGAGRELKRVYPKIELIGVEPAESAILEGKEAGP
                     HKIQGIGTGFVPKTLDTSVYDKVLSISGDEAMETAREVGRKEGILVGISSGAAIAAAL
                     KVAKELGKGKKVLAVVPDNGERYLSTALYQEI"
     gene            2272..2718
                     /locus_tag="ACG842_002606"
     CDS             2272..2718
                     /locus_tag="ACG842_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HFQ0249087.1"
                     /translation="MENKLVEEVIEKLKKENIRITPQRYAVLEYLIEHHSHPTADEIY
                     KALEHRFPNMSVATVYNNLRLFTSIGFVQEMKYGDASSRFDFSSKRHYHVICENCGKI
                     VDFHYPGLEDIEMAASRLTDFEINEHRLELYGLCPDCKESAKKAQM"
     gene            2991..4343
                     /locus_tag="ACG842_002607"
     CDS             2991..4343
                     /locus_tag="ACG842_002607"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidase"
                     /protein_id="HFQ0249088.1"
                     /translation="MKVVVIGCTHAGTAAVKSILKNHPEAEVTVYERNDNISFLSCGI
                     ALYVGGVVKDPAGLFYSNPEELSSLGATVKMEHDVTDVDTENKTVTAKDLKTGETETV
                     SYDKLVMTTGSWPIIPPISGIDAKNVLLCKNYNQANEIIAQAKEAKRVVIVGGGYIGI
                     ELVEAFVESGKQVTLIDGLDRILNKYLDKPFTDILEKELTDRGVTLALGENVTEFITD
                     EEGKVKQVATPTDTFDTDMVILCVGFRPNTKLIEGKVDTLPNGAIKVNEYMQTSNPDI
                     FAAGDSAVVNYNPSGTQNYIPLATNAVRQGLLVGNNLTDHKMAYRGTQGTSGLYLFGW
                     TIGSTGVTKESATLNDLEVQATVFEDNYRPEFMPTTEKVMMELVYEKGTNRIVGAQFM
                     SKYDITQSANTMSLAVQNKMTVEDLALSDFFFQPHFDRPWNYLNLLAQAALGEIETAQ
                     "
     gene            4514..4801
                     /locus_tag="ACG842_002608"
     CDS             4514..4801
                     /locus_tag="ACG842_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5960 family protein"
                     /protein_id="HFQ0249089.1"
                     /translation="MNKEYFVQQDAFLEDYKSASKKLTINIDELADQVLSQLNETQEE
                     YYTMPANDAKDNVSHRFPFSRRIYSDENDGTLNTEYTYEGQPYVLETDDDL"
BASE COUNT         1693 a          813 c         1030 g         1395 t
ORIGIN      
        1 ttaaaagtat aatttaatta attaagtttg ttttaagaag agaacttttt attaagtttt
       61 tttattttga ggaggaattt gaatgcaaga gctgaagacc cgcagtgccc ggcaccatag
      121 acctaaaaga aaaatggtga tcaataaaaa agcgattgct cctttaattg gaacgagtct
      181 tttagttggg ccgatgatgg ttacagcggt accgcaacat gttcatgcag atgaacaagg
      241 atctcaaaca gtcgctgatc ctcaagcgtt tatcgatcaa atcggctggt ctgcgtcaga
      301 agttgctgca agtaatgact tgtatgcttc agtcatgatc gctcaagcgt tgttggaaag
      361 tgcatatggc acttccggat tagctagtgc gccaaattat aatctattcg gtgtgaaagg
      421 aagctataac ggacaggtcg tttatatggc aaccaaagaa tatgtaaacg gacaatgggt
      481 aacgatgaat gagccattcc gaaaatattc ttcttattgg gaatccttcc aagaccatgc
      541 gaatgttctt cgttcaacca gtttttccac gggcgattat cattatgctg gcgtgtggaa
      601 aagcaataca acaagttttt atgatgcaac agcgtacttg acaggtcgtt atgctaccga
      661 tccaaattat ggggccaaat tgaactcatt gattaatact tataatttaa cccgttttga
      721 tacgccatca acaggttcgg ctacaacaac cacaagtaca acaacaacga ctactacgac
      781 gacttcaacg tcatcatctt caacaacaga tgtaaataca aataatggtc agacatatac
      841 agtcgtgtca ggtgatacgc tatgggatat tgcacaaaaa tttggcatgt ctgttgatga
      901 tctaatggcg aaaaatggca tgtctatgtc cagttattct ttattagttg gtcaagaact
      961 aaacgtttga tttagataaa aaggcaaaga aattaccata tatggtatat ctttgtcttt
     1021 tttttgctag tcaaagacga atcactcgcc ataaatgaac agattgtatt atgataagta
     1081 agaagagcaa agacaaggag gaattgaaga tgacagctat ctataactca gtcaccgaat
     1141 tgattggcaa aacgccaatc gtcaaattaa ataaaatcgt accagaagat tcagcagatg
     1201 tttttgtaaa gcttgagttt tttaatccag gaggaagtgt aaaagatcgt attgctttaa
     1261 gtatgataga aaaagcagaa cacgatggtc tactgaaacc aggtgatact atcattgaac
     1321 ctacttctgg aaatacagga atcggattgt cgatggtagg agttgcaaaa ggatataaag
     1381 taatcatcgt gatgcctgag acgatgagta tcgaaagacg tttattgatg aaagggtacg
     1441 gcgcagagtt gattttgact cctggtgcgg atggtatttc tggatcaatc agagaagcag
     1501 aacgtttggc aaaagaaaac ggctactttt taccattaca atttgaaaat gaagcaaatc
     1561 ctcttgtgca tgagaagacg actggtcctg agatacatca agcatttgga gtaaatggct
     1621 tggacgcgtt tgttgcaggt atcggtacag gaggaacgat cactggcgct ggccgagaat
     1681 taaaacgtgt gtatccgaaa atcgaattaa tcggggtaga accagcagaa tctgctattt
     1741 tagaagggaa agaagcggga cctcacaaaa tccaaggaat cggtacaggt tttgttccta
     1801 aaacactgga tacttctgtg tatgataaag tcctttctat tagtggagac gaagcgatgg
     1861 aaacagctag agaagttggg cgaaaagaag ggattttagt aggcatctca tctggcgctg
     1921 caattgctgc cgcgctaaaa gtcgcaaaag aattaggaaa aggcaagaag gtattagctg
     1981 tggttcccga caatggtgaa cgttatttat ctacagccct ttaccaagag atataaggca
     2041 cttggttttt ctccagatag gtttatgtaa aaaactttta aagatgaaaa gaacacacaa
     2101 aaatttatga tgagaaaaat tatttgaata gagacgtaga atagtgaacg cgactgtttt
     2161 acgtcttttt tcattaaaat acaataaaat tgaattttct tttcatgtta agtggatttt
     2221 aaaatgtaaa aatagtatga taagacgaga ataacaacgt ggggtgttta catggaaaat
     2281 aagttagtcg aagaggttat cgaaaagtta aaaaaagaga acattcgaat cacacctcaa
     2341 cggtacgcgg ttttagaata tttgattgaa catcattccc atccaacggc tgatgagatt
     2401 tataaagcac ttgaacatcg gtttccaaat atgagtgttg cgactgttta caataacctt
     2461 cgcttattta cttcgattgg atttgttcaa gaaatgaagt acggcgatgc ttctagccga
     2521 tttgatttca gttcaaagcg tcattatcat gtaatttgtg aaaactgcgg taagattgtt
     2581 gattttcatt acccagggct tgaagatata gaaatggctg caagcagact gactgatttt
     2641 gagattaatg aacatcggtt ggaattgtat ggactctgtc cggattgtaa agagtcagca
     2701 aaaaaggcgc aaatgtaaaa acagtttcta caagtgttat acctaaaaaa agctagctgt
     2761 gtaaaaaaaa cgctagcttt tttatttttt tgtcttttat tcacaatctt agatataaaa
     2821 gtgaatatta ttaaaataag aatcttgtag atattgggtt cttatgcttg aaaaggaatg
     2881 aaactacaaa caaacctttg tgaattcaca agcattaaac tgttgtatcc gttttatttc
     2941 tgttatagta gttttgtcga aggacaaaat gttaatcgga gggaccataa atgaaagtag
     3001 tagtaattgg ctgtactcat gcaggtactg cagctgtaaa aagtatttta aaaaatcatc
     3061 cagaagcaga agtgacagta tatgaaagaa atgacaatat ttctttctta tcttgcggta
     3121 tcgcacttta tgtcggcgga gtagtaaagg acccagcagg cttgttctac tcaaatcctg
     3181 aagaattatc ttcattagga gcaacagtta aaatggaaca cgatgtaaca gacgtcgata
     3241 cagaaaataa aacagtaacg gcaaaagatc tgaaaacagg agaaacagaa acggtttctt
     3301 atgataaatt agtgatgaca actggttcat ggcctattat cccaccgatt agtggaatcg
     3361 atgcgaaaaa tgtactccta tgcaaaaact acaatcaagc aaatgaaatt attgcacaag
     3421 cgaaagaagc aaaacgtgta gtaattgttg gtggtggata tatcggtatt gaattagtag
     3481 aagcttttgt cgaatctgga aaacaagtga cattgattga tggattagat cgcatcttga
     3541 ataaatatct agataaacca ttcacagata tcttagaaaa agaattgaca gaccgcggtg
     3601 ttactcttgc gctaggcgaa aatgttactg aatttatcac agatgaagaa ggaaaagtga
     3661 aacaagtagc tacaccaaca gatacatttg atacagatat ggtcatttta tgtgtgggct
     3721 tccgtccaaa tacaaaactg atcgaaggaa aagtagatac actgccaaac ggggcaatca
     3781 aagtgaatga atacatgcag acaagtaatc cagacatttt tgcagctgga gactctgcag
     3841 tagtcaacta caatccaagt ggcacgcaaa actatattcc tttggcaaca aatgccgttc
     3901 gtcaaggttt gcttgtggga aataacttga cagatcataa aatggcgtat cgtggtactc
     3961 aaggaacttc aggactttac ctatttggct ggacgatcgg ttcgacaggt gtgaccaaag
     4021 aaagcgcaac tttgaacgat ctagaagtcc aagcgactgt atttgaagat aattatcgtc
     4081 cagagttcat gccaactact gaaaaagtaa tgatggagct agtctatgaa aaaggaacga
     4141 atcgtatcgt aggtgctcag ttcatgtcca aatacgacat tacacaatca gcaaatacca
     4201 tgtctttagc tgttcaaaat aaaatgactg tagaagatct cgcactttca gatttcttct
     4261 tccaaccaca ttttgaccgc ccatggaatt acttgaacct acttgcacaa gcagcgctag
     4321 gcgaaatcga aactgcacaa taaagaaaaa gggttgtgac acaattttgt cacgaccctc
     4381 tgtctttttt tgtgaaaagt acaattagtt gtctttcttc gaattttttg tcacaataag
     4441 cagcaaagat aaagctgtta cataaaagaa caagcagctt tatttgattt tgaaaagaaa
     4501 ggtgagaaag aagatgaata aagaatattt tgtccaacaa gatgcatttt tagaagatta
     4561 taaaagtgct tcgaagaaat taacgatcaa tattgatgag ttggcagatc aagtactctc
     4621 tcagttgaat gaaacgcaag aggaatatta cacgatgcca gcaaatgacg ccaaagataa
     4681 tgtttctcat cgttttcctt tcagtagacg catctactca gatgaaaatg acggcacatt
     4741 gaatacagag tacacatatg aaggtcagcc atatgtgcta gaaactgatg atgatcttta
     4801 aagacagttt ttgaaaaggc cgctcatcct taagtgatga caaaaaatca tcacaattac
     4861 cgatgactgt ctctttacta tcttttaatc agaaaaaggg gttatgacaa gctttgtcaa
     4921 cccctttttt t
//
LOCUS       DAZKLM010000134         4892 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3764
            SAMEA115706913-rid22582563.denovo.139, whole genome shotgun
            sequence.
ACCESSION   DAZKLM010000134 DAZKLM010000000
VERSION     DAZKLM010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706913
            Sequence Read Archive: ERR13196647
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4892)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4892)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287823.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287823.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,004
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,849
            CDSs (with protein)               :: 2,849
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 41 of 96
            Pseudo Genes (incomplete)         :: 52 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 15 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4892
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706913-rid22582563.denovo.139"
                     /isolate="VRE3764"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(287..649)
                     /locus_tag="ACG842_002609"
     CDS             complement(287..649)
                     /locus_tag="ACG842_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0249090.1"
                     /translation="MASKRVAGTIMLHLEDGTKKFLVHSIDDKLEFALAELSEGKTGL
                     ANILNFLKEIVHIDVSKISLMELTNTHIKEENVPLFVFETEQKNQREELGEGYIWEEP
                     GQMRSVLGTYEVEGVPFF"
     gene            complement(723..1103)
                     /locus_tag="ACG842_002610"
     CDS             complement(723..1103)
                     /locus_tag="ACG842_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvfD/Ygs/GSP13 family RNA-binding
                     post-transcriptional regulator"
                     /protein_id="HFQ0249091.1"
                     /translation="MTYKIGQIIEGKVTGIQPYGAFVSLDNETQGLIHVSEVQSGYTK
                     NIHSLLKVGQPVTVQIIDIDEYSKKISLSLRTLEKTNPAVPYRRKRYFTNKNRKIGFT
                     TIAEQLPIWIEEALSELERKENKK"
     gene            1185..2360
                     /locus_tag="ACG842_002611"
     CDS             1185..2360
                     /locus_tag="ACG842_002611"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0047804 - cysteine-S-conjugate beta-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MalY/PatB family protein"
                     /protein_id="HFQ0249092.1"
                     /translation="MIEFDKQISRKDTESVKWDAIAETYQMDDLLPLWVADMDFLSPG
                     GVAKAFSEYIKHGIFGYATLSDKLYQAIIHWERQHHAVELTKENIVFTSGVLTSLAAA
                     VQTFTKPGDSILIHDPVYPPFSSIIETNQRHIVRSSLLEKNNHFIMDLSDMEKKIEEN
                     NVKAMILCNPHNPGGRVWSKEELKQLSELCLKHQVILFSDEIHQDLTLFDHTFTSMLT
                     IDPNLDDLLVAFTSATKTFNLAAIKNSMVFIKNPEMKADFEKHLVMNQQHEINTFGLI
                     GTQAAYETGGEWLSQLIPYLEKNVQTVNQFFEQYLPKVRIMNPEGTYLMWLDFTAYIS
                     DDQELENTLVRKGKVVLNPGITFGPSGHGHMRLNIACPEETLLEGLNRIKYALETID"
     gene            complement(2437..3021)
                     /locus_tag="ACG842_002612"
     CDS             complement(2437..3021)
                     /locus_tag="ACG842_002612"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFQ0249093.1"
                     /translation="MAFPQLDLKSEKGPKAVIKTNRGDITVQLFPELAPKTVQNFIEL
                     SKKGYYDGVIFHRVIPDFMIQGGDPTGTGMGGESIYGESFEDEFSRELFNLRGALSMA
                     NSGPNTNGSQFFIVNNTNVPANMLGQLEGAGFPSEIIEAYKGGGTPWLDFRHTVFGHV
                     LEGMDTVDEIANVQRGPQDRPVHDVVIETIEISE"
     gene            3222..4220
                     /locus_tag="ACG842_002613"
     CDS             3222..4220
                     /locus_tag="ACG842_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFQ0249094.1"
                     /translation="MEIYDITIIGAGPVGMFAAFYAGMRQAKTKIIDSLPQLGGQLAT
                     LYPEKYIYDIPGYPAIKASELIDQLEKQLTTFNHTFHLKEEVLSLTREDEVIEITTNK
                     GIHYSKAVILALGNGSFQPRKLNLDNAESFENHGLDYFVNDLMKYAGKKVAIAGGGDS
                     AIDWTLMLEPIASEVYLIHRRPEFRAHEHSVSRLKSSSVNLLTPYLIDGLSGNNGELT
                     DIRLKKVKSDETIDLMIDSLIVNYGFSSNLEHLSSWGLDSTRNAITVKSDMSTSIPGV
                     YAAGDICTYEGKVKLIATGLGEAPTAVNNALHYINPKERTQPGHSTSLYDKNLRPS"
     gene            4332..4841
                     /locus_tag="ACG842_002614"
     CDS             4332..4841
                     /locus_tag="ACG842_002614"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008962 - phosphatidylglycerophosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HFQ0249095.1"
                     /translation="MVVKTETLEEKARELLKERGVTVEDIAQLVMFLQKDYISDLTLA
                     DCTESVNSVLTKREVHNAIITGIQLDILAEKKKLMSPLQHIIEDDEGLYGIDEILALS
                     IVNVYGTIGFTNYGYIDKVKPGILKELNSHDGVHVHTFLDDIVGAIAAAAASRLAHKE
                     PEKRSRLTQ"
BASE COUNT         1553 a          914 c          851 g         1574 t
ORIGIN      
        1 ttttttaaat tttttagaaa tgtgatataa catccacttc agaatcaacg ctgttaaatt
       61 tatcatgtat ttcttccttg gtcaactata aattaagcat tactttgtga aatgattttt
      121 cgatcaatta ttattattcg ttatacaacc gttttcaaca tattttgaaa ataaaatgca
      181 ctaaaaaact atctttagca gaacatttca ttgtattttt tggaccaaac aataaaaaaa
      241 gaagtttgaa aaaaattcaa acttctttgt tcgcttaatc tgtattttag aagaaaggca
      301 ctccctctac ttcgtaagtt ccaagaacag agcgcatttg acctggttct tcccaaatat
      361 atccttcgcc taattcttct ctttgattct tttgttcagt ttcaaagaca aacaacggaa
      421 cattttcctc tttaatatgt gtatttgtca attccatcaa actaatcttg ctaacatcga
      481 tatgaactat ttcttttaag aaattaagta tattcgctaa accagtcttt ccttcagaaa
      541 gttccgctaa cgcaaattcc aacttgtcat caattgaatg gaccaaaaat tttttagtac
      601 catcctccaa atgcaacata attgtaccag caactctttt gcttgccaaa atttccacct
      661 cgatcaactt gttctatggc ttacataaaa attctagcac attttcagaa aaaatacact
      721 ttttatttct tattttcttt ccgttctagt tcagataatg cttcttctat ccaaataggt
      781 aactgctccg caatagtggt aaatccgatc ttacgatttt tattcgtgaa ataccttttt
      841 ctgcgatatg gaacagctgg atttgttttt tctaacgttc ttaaagataa actgattttt
      901 ttggaatatt catcaatgtc gatgatctga actgttactg gctgtccgac ttttaacaat
      961 gaatggatat ttttcgtata accggattgt acttctgaaa catgaataag tccttgcgtc
     1021 tcgttatcaa gagaaacgaa tgcgccataa ggctgtatcc ctgtcacttt tccttcaatt
     1081 atttgtccaa ttttataagt catctacttc cctctcttcg catttatcat tatactgtaa
     1141 atagctcaaa aaaagaagcg acgaaaatga ctggaggtct taagatgatc gaatttgata
     1201 aacagatttc aagaaaagat acagaaagtg taaaatggga cgcaattgct gagacatatc
     1261 aaatggatga cttacttcct ttgtgggtag cagatatgga cttcttgtct cccggtgggg
     1321 tagctaaagc gtttagtgaa tacatcaaac atggaatctt tggttatgcc actctgtctg
     1381 ataaactata tcaggctatc attcattggg aacgccaaca ccatgctgtg gagttgacga
     1441 aagaaaatat cgtttttaca agtggtgtgt taaccagttt agctgcagca gtacaaactt
     1501 ttacaaaacc aggcgattct attttgattc atgacccagt ttatcctcca ttttcctcta
     1561 ttatagaaac aaatcaacgt cacatcgttc gaagctcttt gctagaaaaa aataatcatt
     1621 tcatcatgga cttgtctgac atggagaaaa aaatcgaaga aaacaatgta aaagcaatga
     1681 ttttatgcaa tcctcacaat cctggagggc gtgtttggag caaagaagaa ttgaaacagt
     1741 taagcgaatt gtgtttaaaa catcaagtga ttctgtttag tgacgagatc catcaagatt
     1801 taactttatt cgatcataca tttacttcca tgctaacgat tgatcctaat ttagatgatc
     1861 tactagttgc ttttacttcc gcaaccaaga ctttcaatct agctgcaatc aaaaattcaa
     1921 tggtatttat caaaaatcca gagatgaaag ctgattttga aaaacattta gtgatgaacc
     1981 aacagcacga aatcaacact tttggactga ttggaaccca agcggcttat gagacgggtg
     2041 gagaatggtt aagccaactg attccttatc tagaaaaaaa tgttcaaaca gttaatcagt
     2101 tttttgaaca gtatcttccg aaagtgagaa taatgaaccc tgaaggaact tacttaatgt
     2161 ggttagattt taccgcctat atttcagacg accaagaatt ggagaacact cttgttcgga
     2221 aaggaaaagt cgtattaaat cctggcatca cgtttggtcc ttccggtcat gggcatatgc
     2281 gcttgaatat tgcttgtcca gaagaaactt tactggaagg acttaatcga atcaagtatg
     2341 ctttagagac aatcgattaa atcatttatt caaaataaaa ggacgagacc ttactaggtt
     2401 tcgtcctttt tcataattac tccgtaccat accaaactat tctgaaattt caattgtttc
     2461 aatcactaca tcatgtacag gtcggtcttg tggaccacgt tgtacatttg cgatttcatc
     2521 tactgtgtcc atcccttcta gtacatgtcc aaagactgta tgtcggaaat ctagccaagg
     2581 agttccgcca cctttatacg cttcaatgat ttctgatggg aatccagcac cttctagttg
     2641 tccaagcata ttagctggca catttgtgtt atttacaata aagaattggc ttccgtttgt
     2701 gtttggacct gaatttgcca tagataaagc tccgcgaagg ttgaacagtt cacgactgaa
     2761 ttcatcttca aaactttcgc cataaatact ttcgccaccc attcctgttc cagttggatc
     2821 gccgccttgg atcatgaaat caggaataac gcggtgaaaa atcacgccat cataataacc
     2881 ttttttggaa agttcaatga aattttgaac cgtcttcggt gctagttcag ggaataattg
     2941 aacagtaatg tcaccacggt ttgttttgat aacagctttt ggtccttttt cactttttaa
     3001 atctagttgt gggaatgcca tattgtttcc tcctaatatt tgcatacttc ataataataa
     3061 taacataata cctaacctct cgtaaaaata ctagatttgt tttcttttat ttttcatcac
     3121 tagatacatt ctataactta ccgtttcgat ttcgaattca aacatttttg agaagcattc
     3181 tcaattaaat tctgctatag tgtgaaaagg gaggaatttt tatggaaatc tatgacatta
     3241 caatcatcgg tgcaggtcct gtagggatgt ttgccgcttt ttacgcagga atgcgccaag
     3301 ctaaaacaaa aatcattgac agtctacctc aacttggtgg tcaattagca actttgtatc
     3361 ccgaaaaata tatttacgat attcctgggt atccagcaat aaaagcaagt gaattaatcg
     3421 atcaattaga aaaacaatta actactttta accatacatt ccatttaaaa gaagaagttc
     3481 tctcgcttac tcgtgaagac gaagttatcg agattactac aaataaaggc atccattatt
     3541 ccaaagcagt cattttagct ttaggaaatg gttcattcca gccaagaaaa ttgaatttag
     3601 ataatgctga atcatttgaa aaccacggat tggattattt cgtgaatgat ttgatgaagt
     3661 atgctggaaa aaaagtcgct attgctggag gtggtgattc tgctatcgat tggacgttga
     3721 tgttagagcc tattgcaagc gaagtttact tgattcatcg tcgcccggaa tttcgtgcgc
     3781 acgaacatag tgtatcacgc ttgaaatctt catctgtcaa cttgcttact ccctatctca
     3841 tcgatggatt atcaggcaat aatggggaac tgacagatat ccggttaaaa aaagtgaaaa
     3901 gtgacgagac gattgatttg atgattgatt ctctgatcgt caattatgga ttctcttcta
     3961 atttagaaca tctttcttct tggggacttg atagtaccag aaatgccatt actgtaaaat
     4021 cagatatgtc tacttctatt ccaggcgttt atgcagctgg tgacatctgt acctatgaag
     4081 gaaaagtgaa actgattgct actgggctcg gtgaagcacc tactgcagtg aacaacgctt
     4141 tacactatat taatccaaag gaacgaactc agcctggtca cagcacaagc ttatacgata
     4201 aaaatcttcg tcctagttga ttttttatat ctctttgcat ttcaagttaa acccgaaagc
     4261 agtttcttgc atttttttca tgttccgtta acatgaaata gagaaagaaa aaataaaggg
     4321 ggagcaataa tatggtcgtc aaaactgaga ctttagaaga aaaggcaaga gaactattga
     4381 aggaacgtgg cgtaactgta gaagatatcg cacaacttgt catgttttta caaaaagact
     4441 atatttctga tttgactttg gcggattgta ctgaaagtgt caacagcgtg ctaacaaagc
     4501 gtgaagtaca taacgccatt atcactggaa tccaattaga tatattagca gaaaagaaaa
     4561 aattgatgag tccgttgcaa catattatcg aagatgacga aggcctttac ggtatcgatg
     4621 agatcctagc cttgtcaatc gtgaatgttt acggtacgat cggctttacg aattatggtt
     4681 atatcgacaa ggtgaaacct ggtattttaa aagaactaaa cagccacgat ggcgttcatg
     4741 tccatacctt cttagatgat atcgtcggag cgatcgccgc tgctgctgcc agtcgattag
     4801 cacacaaaga accagaaaaa agaagtcgcc ttactcaata attcaacaaa aaaagaaaga
     4861 aaacgtctat ttagacgagt tctttctttt tt
//
LOCUS       DAZKLM010000135         4775 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3764
            SAMEA115706913-rid22582563.denovo.141, whole genome shotgun
            sequence.
ACCESSION   DAZKLM010000135 DAZKLM010000000
VERSION     DAZKLM010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706913
            Sequence Read Archive: ERR13196647
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4775)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4775)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287823.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287823.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,004
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,849
            CDSs (with protein)               :: 2,849
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 41 of 96
            Pseudo Genes (incomplete)         :: 52 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 15 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4775
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706913-rid22582563.denovo.141"
                     /isolate="VRE3764"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(138..1100)
                     /locus_tag="ACG842_002615"
     CDS             complement(138..1100)
                     /locus_tag="ACG842_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004233175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFQ0249096.1"
                     /translation="MVAKLTDVAELAGVSPTTVSRVINNKGYLSEKTKQKVHEAMKTL
                     GYKPNNLARGLQGKSPQLIGLIFPNISNIFYSELIEYLEIELFKHGYKTIICNSQNDP
                     AKEREYIEMLEANQVDGIISSSHNLGIADYERVRAPIVAFDRNLAPNVSIVSSDNFEG
                     GKLAAKTLQKNGCQNTIMITGNDNTDSPTELRALGFSFQNPNGKIFKIPNNLSTIRRE
                     MEIKSTIINHKPDGIFVSDDLTAILTMKIAHQLKLNIPEDLKIIGYDGTSFIEKFFPQ
                     LTTIRQPIDEIASLIVDILLKKIKGEKTNKDYILPISILSGGSI"
     gene            complement(1102..2541)
                     /locus_tag="ACG842_002616"
     CDS             complement(1102..2541)
                     /locus_tag="ACG842_002616"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004564 - beta-fructofuranosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HFQ0249097.1"
                     /translation="MNLPREIRYRAYSDWTKDEMDKIKDNVKRSPWRASYHIEPKTGL
                     LNDPNGFSFFNGKYTLFYQNWPFGAAHGLKEWVHNESDDLVHFHVTGAELLPDTKRDS
                     HGAYSGSAYEIEGNLFLLYTGNVRNENWERDTLQIGAWMDKDYNITKGENVLIHKPAD
                     VTDHFRDPQMFNYEGQLYAIVGAQNLEKSGFIKLYKADDNNVETWEEVGNLDFGGTGS
                     EYMIECPNLVFVDKKPVLIYCPQGLDKSELNYGNIYPNTFKICQSLDIEKVKLVGASE
                     IQNLDYGFEAYATQGFNTPDGRALIVSWIGLPDIDYPTDKYDYQGAMSLVKELSIKDG
                     KLYQYPVEAITSLRASSEQFTAKAETNNTYELELTFPPNQKSELLLFSDSKGNGLSLI
                     VDTKEGKIILDRSKAGIQYATDFGTIRECSIDTKETTANIFVDNSIIEIFINKGEKVF
                     TSRVFPENHQNGIQIKSGEPTGKYFELKY"
     gene            2726..4672
                     /locus_tag="ACG842_002617"
     CDS             2726..4672
                     /locus_tag="ACG842_002617"
                     /EC_number="2.7.1.211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-specific PTS transporter subunit IIBC"
                     /protein_id="HFQ0249098.1"
                     /translation="MNYSKVASDVIQAVGKDNLIAAAHCATRLRLVLKDDTKVNQKAL
                     DENPDVKGTFKIDGQYQVIIGAGDVNFVYDELIKKTGLSEISTDDLKQIASKDKKFNP
                     IMALIKLLSDIFVPIIPALVAGGLLMALQNFLTAAGLFGPKSIEEMYPAIEGISSMIQ
                     LMSAAPFMFLPILVGISAAKRFGANQFLGAAIGMIMTTPDLGGSTEYWNIFGYHVSQT
                     NYAYQVIPVLAAVWILSILEKFFHKKLPSSIDFTFTPLLSVMITGFLTFTVIGPVMLL
                     LSNGITDIIVWLYNTTGFIGMGIFGGTYSLIVMTGLHQSFPAIETQLLSAWTNGIGHG
                     DFIFVVASMANVAQGAATFAIWFLTKNSKTKSLASSAGVSALLGITEPALFGVNLKYR
                     FPFFCALIGSGIATAVAGLLKVVAVSLGSAGFLGFLSINATSIPFYVLCELISFVVAF
                     AITYFYGKTKAAGIFKAEEIAEQTPVNSSKVATTDPISTDVESTQTKVSSETIVSPIS
                     GKVISLSSVNDPVFSSEAMGKGIAIKPNGNTVHSPVDGTIQLTFETGHAYGIKSNNGA
                     EVLIHIGIDTVSMGGKGFTQKVTTNQKVRKGDILGAFDRSMIEKEGLDDTTMIIVTNT
                     SDYSEVNIVAENTVTEGTDLLEIK"
BASE COUNT         1519 a          816 c          780 g         1660 t
ORIGIN      
        1 gatttctcca ctccaaaaaa gtctaacttt tgggggtcac tacaagttga caactccttt
       61 ttgtttacaa atcaattgga tatctacaaa ttgtattttc taacgaattt tatagacatt
      121 aaaaaattaa tttaacgtta aatacttcct ccagaaagta ttgagattgg taaaatataa
      181 tccttatttg tcttttcgcc tttaattttt ttcagaagaa tatcaacaat aagacttgca
      241 atttcatcaa ttggttgtct tattgttgtc aattgtggga aaaatttctc aataaaagag
      301 gtaccatcgt atccaataat ttttaaatct tctgggatat taagtttaag ttgatgagca
      361 attttcatag tgagaatggc tgttaaatca tcagaaacaa aaataccatc tggtttgtga
      421 tttataatag ttgactttat ttccatttca cgacggatag tagacagatt attaggaatt
      481 ttgaaaattt ttccatttgg attttgaaaa gaaaaaccta gtgcacgtaa ttctgtggga
      541 gaatccgtgt tatcatttcc tgtaatcata atggtatttt gacaaccatt tttttgtaaa
      601 gtcttagctg caagttttcc cccttcaaag ttatctgaag aaacaatgga aacattagga
      661 gctaaatttc tatcaaaagc aacaataggt gctctcacac gctcataatc agcaattcca
      721 agattatggc ttgatgagat tatgccatca acttggttgg cttcaagcat ttcaatgtat
      781 tctctctctt tagcaggatc attttgacta ttgcaaataa tcgttttata tccatgttta
      841 aataactcta tttcgagata ctcaatgagt tcagagtaaa agatattact aatattagga
      901 aaaataagac caataagctg aggtgacttt ccttgaagtc cacgtgctaa attatttggt
      961 ttataaccaa gagttttcat agcttcatga actttttgtt ttgttttttc tgataaatat
     1021 cccttattat taatgacgcg tgaaacggtt gttgggctaa ctccagctaa ttcagcgaca
     1081 tcagttaatt ttgctaccat tttaatattt taattcaaag tattttccag taggttcgcc
     1141 tgatttgatt tgaataccat tttggtgatt ttcagggaat acacgactag taaacacttt
     1201 ttctccttta ttaataaaaa tctcaatgat tgagttatct acaaaaatgt tagcagttgt
     1261 ttctttagta tcaattgagc attcacgaat cgtaccaaag tcagtggcat attgaatgcc
     1321 tgctttgctg cgatctaaaa taatttttcc ttctttggta tctacaatca agctaagtcc
     1381 atttccttta ctatcagaga aaagaagtaa ctccgatttc tgatttggag gaaaagttag
     1441 ttcaagctca taagtattat ttgtttctgc ttttgctgta aattgttcag aactagcacg
     1501 taatgatgta atagcttcta ctggatattg gtaaagttta ccatctttta ttgaaagttc
     1561 ttttacaaga ctcattgctc cctgataatc atacttatca gttggataat cgatatcagg
     1621 tagtccaatc caactgacaa tcagagcacg accatcagga gtattgaatc cttgagtcgc
     1681 atacgcttca aatccataat caagattttg aatttctgaa gcgcccacaa gttttacttt
     1741 ttctatgtca agcgattgac agatcttaaa agtattcgga tagatattcc cataatttag
     1801 ctcagattta tccaaccctt gaggacaata aatgagaaca ggttttttgt ctacaaatac
     1861 tagatttgga cactcaatca tgtattctga accagtgccg ccaaaatcaa ggttgccaac
     1921 ttcctcccat gtttccacgt tattgtcatc cgctttgtaa agtttgataa agcctgattt
     1981 ttctaaattt tgtgctccaa caatcgcata caattgtcct tcataattaa acatttgtgg
     2041 atcacgaaaa tgatcagtta catcagcagg tttatgaata agaacattct cacctttcgt
     2101 aatgttgtag tctttatcca tccatgctcc gatttgaagt gtatcacgtt cccaattttc
     2161 atttcgaacg tttcctgtat agagtaagaa tagattcccc tctatttcat atgcagaacc
     2221 ggaataagcg ccgtggctat ctcgttttgt gtctggaaga agttctgctc ctgttacatg
     2281 aaaatgaact aaatcatctg attcattgtg tacccattct ttcaacccat gagcagctcc
     2341 aaatggccaa ttttgataaa aaagcgtata ttttccgtta aaaaaagaaa aaccattagg
     2401 atcgtttaac aatcctgttt taggttcgat atgatagcta gcacgccaag gtgatcgttt
     2461 tacattatcc ttaattttat ccatctcatc tttagtccaa tcagaataag cacgatatcg
     2521 tatttctcga ggtaagttca tattttactc cttcaattta tctttttaca actagtatgg
     2581 taaacgtttt ccgttgaaaa atcaatcatt tcatttaaaa aaataaacta tatgacaaac
     2641 gtttgacata tttttaagat atgctaaaat aaatttgcat ttcagtgaac cgttttcacg
     2701 taaaaataaa aaaggagtga ttcgaatgaa ttacagtaaa gtagcttccg acgtaataca
     2761 agcggtcgga aaagataacc ttattgcagc tgcacactgc gctacacgtc ttcgtctcgt
     2821 actaaaagat gacacgaaag tgaatcaaaa agctttagac gaaaatccag atgtgaaagg
     2881 cacatttaaa attgatggac aatatcaagt gattatcggt gcaggtgatg ttaatttcgt
     2941 ttatgacgaa cttatcaaaa aaactggact ttcggaaatt tcaacagatg atttaaaaca
     3001 aattgctagt aaagataaaa aattcaaccc cattatggca ttaattaaat tattatcaga
     3061 tatttttgtt ccaattatac ctgcccttgt tgcaggtggt ttattaatgg cattacaaaa
     3121 ctttttaaca gctgctggac tttttggacc aaaatcaatt gaagaaatgt atcctgcgat
     3181 tgaaggtatt tcctcaatga tccaactgat gtccgctgca ccattcatgt tcttaccaat
     3241 tttagtaggt atttcagctg ctaaacgttt tggagcaaac caatttcttg gtgctgcaat
     3301 tggtatgatc atgacaacac cagatcttgg tggctcaact gaatattgga atatttttgg
     3361 ttatcatgtt tcacaaacaa actatgctta tcaagttatt ccagttttag cagctgtttg
     3421 gatattatct atattagaaa aattctttca taaaaaattg ccatcatcta ttgactttac
     3481 attcacgcca ttattatcag taatgattac tggatttttg actttcacag ttattggccc
     3541 tgtaatgtta ttgttgtcta atggaattac agacattatt gtttggctat ataacactac
     3601 tggatttatt ggaatgggta tttttggagg aacatattca cttatagtca tgactggttt
     3661 acatcaatca tttccagcta ttgaaactca attactttca gcgtggacaa atggtatcgg
     3721 acatggagac tttatctttg tagttgcatc tatggcaaac gttgctcaag gtgctgcaac
     3781 atttgccatt tggttcttaa ctaaaaattc taagacaaaa agtcttgctt cctctgcggg
     3841 tgtctcagca cttcttggaa ttacagagcc tgccttgttc ggagttaact taaaataccg
     3901 ctttccattc ttttgtgctt taattggttc aggaattgct acagcagtag caggattact
     3961 taaagtagtt gctgtttcac ttggttcagc aggtttcttg ggatttcttt caattaatgc
     4021 aacatctatt ccattttacg tattgtgtga attgattagc tttgtagttg cttttgcaat
     4081 tacttacttc tacggtaaaa caaaagctgc tggtatcttt aaagcagaag aaattgctga
     4141 acaaacacca gttaattctt caaaagtagc aacaacagat ccaatatcta ctgatgttga
     4201 atcaacacaa acaaaagttt catcagaaac aattgttagt cccatttctg gtaaagtgat
     4261 tagtctttct tcagtaaatg atccagtctt ttcatcagaa gctatgggaa aagggattgc
     4321 cattaaacca aatggaaaca cggtccattc tccagtagat ggtactattc aactcacgtt
     4381 tgaaactgga catgcttatg gtattaaatc aaataatggt gctgaagttc taattcatat
     4441 tggtattgat actgtatcaa tgggaggaaa aggatttaca caaaaagtta ctactaatca
     4501 aaaagttaga aaaggcgata ttcttggagc atttgaccgt tcaatgattg agaaagaagg
     4561 tcttgatgat acaacaatga ttattgtgac caatacttcg gattattcag aagtaaatat
     4621 agtagctgaa aatacagtca ctgagggtac tgatttactt gaaatcaaat aaatatattt
     4681 ccctgagtaa agatggaatt ttctttccaa aatcaaataa tatagattaa tcaaatattt
     4741 gagaataaca ttttatttaa tcttttcata tatca
//
LOCUS       DAZKLM010000136         4757 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3764
            SAMEA115706913-rid22582563.denovo.142, whole genome shotgun
            sequence.
ACCESSION   DAZKLM010000136 DAZKLM010000000
VERSION     DAZKLM010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706913
            Sequence Read Archive: ERR13196647
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4757)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4757)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287823.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287823.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,004
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,849
            CDSs (with protein)               :: 2,849
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 41 of 96
            Pseudo Genes (incomplete)         :: 52 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 15 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4757
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706913-rid22582563.denovo.142"
                     /isolate="VRE3764"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            51..746
                     /gene="vanR-A"
                     /locus_tag="ACG842_002618"
     CDS             51..746
                     /gene="vanR-A"
                     /locus_tag="ACG842_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance response regulator
                     transcription factor VanR-A"
                     /protein_id="HFQ0249099.1"
                     /translation="MSDKILIVDDEHEIADLVELYLKNENYTVFKYYTAKEALECIDK
                     SEIDLAILDIMLPGTSGLTICQKIRDKHTYPIIMLTGKDTEVDKITGLTIGADDYITK
                     PFRPLELIARVKAQLRRYKKFSGVKEQNENVIVHSGLVINVNTHECYLNEKQLSLTPT
                     EFSILRILCENKGNVVSSELLFHEIWGDEYFSKSNNTITVHIRHLREKMNDTIDNPKY
                     IKTVWGVGYKIEK"
     gene            724..1878
                     /locus_tag="ACG842_002619"
     CDS             724..1878
                     /locus_tag="ACG842_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance histidine kinase VanS-A"
                     /protein_id="HFQ0249100.1"
                     /translation="MVIKLKNKKNDYSKLERKLYMYIVAIVVVAIVFVLYIRSMIRGK
                     LGDWILSILENKYDLNHLDAMKLYQYSIRNNIDIFIYVAIVISILILCRVMLSKFAKY
                     FDEINTGIDVLIQNEDKQIELSAEMDVMEQKLNTLKRTLEKREQDAKLAEQRKNDVVM
                     YLAHDIKTPLTSIIGYLSLLDEAPDMPVDQKAKYVHITLDKAYRLEQLIDEFFEITRY
                     NLQTITLTKTHIDLYYMLVQMTDEFYPQLSAHGKQAVIHAPEDLTVSGDPDKLARVFN
                     NILKNAAAYSEDNSIIDITAGLSGDVVSIEFKNTGSIPKDKLAAIFEKFYRLDNARSS
                     DTGGAGLGLAIAKEIIVQHGGQIYAESNDNYTTFRVELPAMPDLVDKRRS"
     gene            2093..3061
                     /locus_tag="ACG842_002620"
     CDS             2093..3061
                     /locus_tag="ACG842_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-A"
                     /protein_id="HFQ0249101.1"
                     /translation="MNNIGITVYGCEQDEADAFHALSPRFGVMATIINANVSESNAKS
                     APFNQCISVGHKSEISASILLALKRAGVKYISTRSIGCNHIDTTAAKRMGITVDNVAY
                     SPDSVADYTMMLILMAVRNVKSIVRSVEKHDFRLDSDRGKVLSDMTVGVVGTGQIGKA
                     VIERLRGFGCKVLAYSRSRSIEVNYVPFDELLQNSDIVTLHVPLNTDTHYIISHEQIQ
                     RMKQGAFLINTGRGPLVDTYELVKALENGKLGGAALDVLEGEEEFFYSDCTQKPIDNQ
                     FLLKLQRMPNVIITPHTAYYTEQALRDTVEKTIKNCLDFERRQEHE"
     gene            3054..4085
                     /gene="vanA"
                     /locus_tag="ACG842_002621"
     CDS             3054..4085
                     /gene="vanA"
                     /locus_tag="ACG842_002621"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanA"
                     /protein_id="HFQ0249102.1"
                     /translation="MNRIKVAILFGGCSEEHDVSVKSAIEIAANINKEKYEPLYIGIT
                     KSGVWKMCEKPCAEWENDNCYSAVLSPDKKMHGLLVKKNHEYEINHVDVAFSALHGKS
                     GEDGSIQGLFELSGIPFVGCDIQSSAICMDKSLTYIVAKNAGIATPAFWVINKDDRPV
                     AATFTYPVFVKPARSGSSFGVKKVNSADELDYAIESARQYDSKILIEQAVSGCEVGCA
                     VLGNSAALVVGEVDQIRLQYGIFRIHQEVEPEKGSENAVITVPADLSAEERGRIQETA
                     KKIYKALGCRGLARVDMFLQDNGRIVLNEVNTLPGFTSYSRYPRMMAAAGIALPELID
                     RLIVLALKG"
     gene            4091..4699
                     /locus_tag="ACG842_002622"
     CDS             4091..4699
                     /locus_tag="ACG842_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-A"
                     /protein_id="HFQ0249103.1"
                     /translation="MEIGFTFLDEIVHGVRWDAKYATWDNFTGKPVDGYEVNRIVGTY
                     ELAESLLKAKELAATQGYGLLLWDGYRPKRAVNCFMQWAAQPENNLTKESYYPNIDRT
                     EMISKGYVASKSSHSRGSAIDLTLYRLDTGELVPMGSRFDFMDERSHHAANGISCNEA
                     QNRRRLRSIMENSGFEAYSLEWWHYVLRDEPYPNSYFDFPVK"
BASE COUNT         1471 a          910 c         1115 g         1261 t
ORIGIN      
        1 tataatctga taaattatta agggcaaact tatgtgaaag ggtgataact atgagcgata
       61 aaatacttat tgtggatgat gaacatgaaa ttgccgattt ggttgaatta tacttaaaaa
      121 acgagaatta tacggttttc aaatactata ccgccaaaga agcattggaa tgtatagaca
      181 agtctgagat tgaccttgcc atattggaca tcatgcttcc cggcacaagc ggccttacta
      241 tctgtcaaaa aataagggac aagcacacct atccgattat catgctgacc gggaaagata
      301 cagaggtaga taaaattaca gggttaacaa tcggcgcgga tgattatata acgaagccct
      361 ttcgcccact ggagttaatt gctcgggtaa aggcccagtt gcgccgatac aaaaaattca
      421 gtggagtaaa ggagcagaac gaaaatgtta tcgtccactc cggccttgtc attaatgtta
      481 acacccatga gtgttatctg aacgagaagc agttatccct tactcccacc gagttttcaa
      541 tactgcgaat cctctgtgaa aacaagggga atgtggttag ctccgagctg ctatttcatg
      601 agatatgggg cgacgaatat ttcagcaaga gcaacaacac catcaccgtg catatccggc
      661 atttgcgcga aaaaatgaac gacaccattg ataatccgaa atatataaaa acggtatggg
      721 gggttggtta taaaattgaa aaataaaaaa aacgactatt ccaaactaga acgaaaactt
      781 tacatgtata tcgttgcaat tgttgtggta gcaattgtat tcgtgttgta tattcgttca
      841 atgatccgag ggaaacttgg ggattggatc ttaagtattt tggaaaacaa atatgactta
      901 aatcacctgg acgcgatgaa attatatcaa tattccatac ggaacaatat agatatcttt
      961 atttatgtgg cgattgtcat tagtattctt attctatgtc gcgtcatgct ttcaaaattc
     1021 gcaaaatact ttgacgagat aaataccggc attgatgtac ttattcagaa cgaagataaa
     1081 caaattgagc tttctgcgga aatggatgtt atggaacaaa agctcaacac attaaaacgg
     1141 actctggaaa agcgagagca ggatgcaaag ctggccgaac aaagaaaaaa tgacgttgtt
     1201 atgtacttgg cgcacgatat taaaacgccc cttacatcca ttatcggtta tttgagcctg
     1261 cttgacgagg ctccagacat gccggtagat caaaaggcaa agtatgtgca tatcacgttg
     1321 gacaaagcgt atcgactcga acagctaatc gacgagtttt ttgagattac acggtataac
     1381 ctacaaacga taacgctaac aaaaacgcac atagacctat actatatgct ggtgcagatg
     1441 accgatgaat tttatcctca gctttccgca catggaaaac aggcggttat tcacgccccc
     1501 gaggatctga ccgtgtccgg cgaccctgat aaactcgcga gagtctttaa caacattttg
     1561 aaaaacgccg ctgcatacag tgaggataac agcatcattg acattaccgc gggcctctcc
     1621 ggggatgtgg tgtcaatcga attcaagaac actggaagca tcccaaaaga taagctagct
     1681 gccatatttg aaaagttcta taggctggac aatgctcgtt cttccgatac gggtggcgcg
     1741 ggacttggat tggcgattgc aaaagaaatt attgttcagc atggagggca gatttacgcg
     1801 gaaagcaatg ataactatac gacgtttagg gtagagcttc cagcgatgcc agacttggtt
     1861 gataaaagga ggtcctaaga gatgtatata attttttagg aaaatctcaa ggttatcttt
     1921 actttttctt aggaaattaa caatttaata ttaagaaacg gctcgttctt acacggtaga
     1981 cttaataccg taagaacgag ccgttttcgt tcttcagaga aagatttgac aagattacca
     2041 ttggcatccc cgttttattt ggtgcctttc acagaaaggg ttggtcttaa ttatgaataa
     2101 catcggcatt actgtttatg gatgtgagca ggatgaggca gatgcattcc atgctctttc
     2161 gcctcgcttt ggcgttatgg caacgataat taacgccaac gtgtcggaat ccaacgccaa
     2221 atccgcgcct ttcaatcaat gtatcagtgt gggacataaa tcagagattt ccgcctctat
     2281 tcttcttgcg ctgaagagag ccggtgtgaa atatatttct acccgaagca tcggctgcaa
     2341 tcatatagat acaactgctg ctaagagaat gggcatcact gtcgacaatg tggcgtactc
     2401 gccggatagc gttgccgatt atactatgat gctaattctt atggcagtac gcaacgtaaa
     2461 atcgattgtg cgctctgtgg aaaaacatga tttcaggttg gacagcgacc gtggcaaggt
     2521 actcagcgac atgacagttg gtgtggtggg aacgggccag ataggcaaag cggttattga
     2581 gcggctgcga ggatttggat gtaaagtgtt ggcttatagt cgcagccgaa gtatagaggt
     2641 aaactatgta ccgtttgatg agttgctgca aaatagcgat atcgttacgc ttcatgtgcc
     2701 gctcaatacg gatacgcact atattatcag ccacgaacaa atacagagaa tgaagcaagg
     2761 agcatttctt atcaatactg ggcgcggtcc acttgtagat acctatgagt tggttaaagc
     2821 attagaaaac gggaaactgg gcggtgccgc attggatgta ttggaaggag aggaagagtt
     2881 tttctactct gattgcaccc aaaaaccaat tgataatcaa tttttactta aacttcaaag
     2941 aatgcctaac gtgataatca caccgcatac ggcctattat accgagcaag cgttgcgtga
     3001 taccgttgaa aaaaccatta aaaactgttt ggattttgaa aggagacagg agcatgaata
     3061 gaataaaagt tgcaatactg tttgggggtt gctcagagga gcatgacgta tcggtaaaat
     3121 ctgcaataga gatagccgct aacattaata aagaaaaata cgagccgtta tacattggaa
     3181 ttacgaaatc tggtgtatgg aaaatgtgcg aaaaaccttg cgcggaatgg gaaaacgaca
     3241 attgctattc agctgtactc tcgccggata aaaaaatgca cggattactt gttaaaaaga
     3301 accatgaata tgaaatcaac catgttgatg tagcattttc agctttgcat ggcaagtcag
     3361 gtgaagatgg atccatacaa ggtctgtttg aattgtccgg tatccctttt gtaggctgcg
     3421 atattcaaag ctcagcaatt tgtatggaca aatcgttgac atacatcgtt gcgaaaaatg
     3481 ctgggatagc tactcccgcc ttttgggtta ttaataaaga tgataggccg gtggcagcta
     3541 cgtttaccta tcctgttttt gttaagccgg cgcgttcagg ctcatccttc ggtgtgaaaa
     3601 aagtcaatag cgcggacgaa ttggactacg caattgaatc ggcaagacaa tatgacagca
     3661 aaatcttaat tgagcaggct gtttcgggct gtgaggtcgg ttgtgcggta ttgggaaaca
     3721 gtgccgcgtt agttgttggc gaggtggacc aaatcaggct gcagtacgga atctttcgta
     3781 ttcatcagga agtcgagccg gaaaaaggct ctgaaaacgc agttataacc gttcccgcag
     3841 acctttcagc agaggagcga ggacggatac aggaaacggc aaaaaaaata tataaagcgc
     3901 tcggctgtag aggtctagcc cgtgtggata tgtttttaca agataacggc cgcattgtac
     3961 tgaacgaagt caatactctg cccggtttca cgtcatacag tcgttatccc cgtatgatgg
     4021 ccgctgcagg tattgcactt cccgaactga ttgaccgctt gatcgtatta gcgttaaagg
     4081 ggtgataagc atggaaatag gatttacttt tttagatgaa atagtacacg gtgttcgttg
     4141 ggacgctaaa tatgccactt gggataattt caccggaaaa ccggttgacg gttatgaagt
     4201 aaatcgcatt gtagggacat acgagttggc tgaatcgctt ttgaaggcaa aagaactggc
     4261 tgctacccaa gggtacggat tgcttctatg ggacggttac cgtcctaagc gtgctgtaaa
     4321 ctgttttatg caatgggctg cacagccgga aaataacctg acaaaggaaa gttattatcc
     4381 caatattgac cgaactgaga tgatttcaaa aggatacgtg gcttcaaaat caagccatag
     4441 ccgcggcagt gccattgatc ttacgcttta tcgattagac acgggtgagc ttgtaccaat
     4501 ggggagccga tttgatttta tggatgaacg ctctcatcat gcggcaaatg gaatatcatg
     4561 caatgaagcg caaaatcgca gacgtttgcg ctccatcatg gaaaacagtg ggtttgaagc
     4621 atatagcctc gaatggtggc actatgtatt aagagacgaa ccatacccca atagctattt
     4681 tgatttcccc gttaaataaa cttttaaccg ttgcacggac aaacggttct gttgcaaagt
     4741 tttaaataaa gaataaa
//
LOCUS       DAZKLM010000137         4603 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3764
            SAMEA115706913-rid22582563.denovo.143, whole genome shotgun
            sequence.
ACCESSION   DAZKLM010000137 DAZKLM010000000
VERSION     DAZKLM010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706913
            Sequence Read Archive: ERR13196647
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4603)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4603)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287823.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287823.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,004
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,849
            CDSs (with protein)               :: 2,849
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 41 of 96
            Pseudo Genes (incomplete)         :: 52 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 15 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4603
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706913-rid22582563.denovo.143"
                     /isolate="VRE3764"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            135..1175
                     /locus_tag="ACG842_002623"
     CDS             135..1175
                     /locus_tag="ACG842_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HFQ0249104.1"
                     /translation="MTDFKFFKADRVYNELFYQFPKVFIVSDEYKKMKDSTKIAYMLL
                     KARLEIAISKRQIDEEGNVYFTYTTNELCRVLNCQKQKAIAIKKELESFGLLLQKQMG
                     FNKQLGKNNPNRLYLAELKVSENDIYLLEKFDRENRENVDKSEGMKIIPTLDEKSDAE
                     SLGAQEGMKIIPCQNVDKSEGMKIIPELNNNILDTNRHNIDTEKDRLQDQLLLDNFET
                     IMTNDSIATFVPERVLNLIKTFSSSYSEAQKTVQTIHNAKKKAEIESDISIVFEELDS
                     YYVNAEQELYTTLLKAYQKLKTEKVENIQNLIFVYVKNWFIEKPIAAKVSSEKRLNYA
                     SSPSTITKDWLE"
     gene            1999..2331
                     /gene="traE"
                     /locus_tag="ACG842_002624"
     CDS             1999..2331
                     /gene="traE"
                     /locus_tag="ACG842_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system protein
                     TraE"
                     /protein_id="HFQ0249105.1"
                     /translation="MSYTGILSLKDICHYGKRCTATEKITKKLSTGQSKTVVQCKKYI
                     IQKDKVSEEMIYYIGKQKQIILKDPIPLKELYPTIKHVYDQNGVLIGRRKNGVLRCTA
                     KGMGRLIG"
     gene            2950..3291
                     /locus_tag="ACG842_002625"
     CDS             2950..3291
                     /locus_tag="ACG842_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 family protein"
                     /protein_id="HFQ0249106.1"
                     /translation="MKNYDPNIRLGTHTIKVSFQRWDYKGFVTFRRGGNCKGLDVLAL
                     DEDDLYDQTLTDNPIGFGLLPEDDEGNEWFKMTLMNDNGDELSVEDTWSYLSDYIVSF
                     EIIEFVADKEE"
     gene            complement(3528..>4603)
                     /locus_tag="ACG842_002626"
     CDS             complement(3528..>4603)
                     /locus_tag="ACG842_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HFQ0249107.1"
                     /translation="VIGMESTSMYSFHPSMFFHEDEKLKKLNTLVTIENPFRVKQFSR
                     MFDENKTDRNDALRIADFLRIQRFTTSPIKEEKYMALQRLTRTRYQLIKQLIRTKQHF
                     LENLTYKCNTLAREMRDESTSLFSATLISLMTEDFTLDELAELPLEAFCDLLQEKGKG
                     RFKQPEKIAKAIQRAITMSYRLGALAQESINVVLSVLVREIRALEKNIKELDKAIEQV
                     IVVIPEYQCLTSIPGVGKVYAAGLIAEIGQIERFEDQTKLAKYAGLSWKVNQSGNYQS
                     QNTPLTKQGNRYFRYYLVEAANSVKNYLPEYKAFYQSKYKEVPKHQHKRALVLTARKF
                     TRLVDTLLRKHQLYTPPRSVIEK"
BASE COUNT         1597 a          670 c          882 g         1454 t
ORIGIN      
        1 ttatttctga ttttaggtag tatttaatta aaaaacagtt ttaatgcgtt tgcatttttt
       61 atttatcttt gctattttcg aaaagagtag gtaattttac ataaataaaa caacgtgaaa
      121 gtagaggatc tacaatgact gattttaaat tttttaaggc ggatagagtt tacaacgaat
      181 tattttatca atttccaaaa gtctttattg tttctgacga atacaaaaaa atgaaagatt
      241 caactaagat tgcctatatg cttttaaaag caagattaga gatcgcaatc agcaaacggc
      301 aaatcgatga agaaggtaat gtttatttta cttatacgac aaatgaacta tgtagagtat
      361 taaactgcca aaaacaaaaa gcgatagcaa tcaaaaaaga gttggaatcc tttggtttat
      421 tattacaaaa gcagatggga tttaacaaac agttagggaa aaataatcct aatagactat
      481 atctagcaga attaaaagtc tcagaaaatg atatctactt actcgaaaaa tttgatagag
      541 agaataggga aaacgttgat aaatcagagg gtatgaaaat catacccacc ctcgacgaaa
      601 aatcagacgc tgaatccctt ggggctcaag agggtatgaa aatcataccg tgccaaaacg
      661 ttgataaatc agagggtatg aaaatcatac cagaacttaa taataatata ttagacacta
      721 atagacacaa tatagacact gaaaaagacc gcctacaaga tcaattgttg ttagacaatt
      781 ttgagacaat tatgacaaac gacagcattg ctacgtttgt ccctgaacga gtattaaatt
      841 tgataaaaac attttcttca agttacagtg aagctcaaaa aaccgtccag actattcata
      901 atgcaaagaa aaaagctgaa atagaaagtg atatttcgat agtttttgaa gaactcgata
      961 gttattatgt caatgcagaa caagaattat acacgacact gttaaaagcc tatcaaaaat
     1021 taaaaaccga aaaagtcgaa aatatccaga acctgatttt tgtctatgta aaaaattggt
     1081 ttatcgaaaa accaatagct gctaaagtat caagtgaaaa acgtttgaat tatgcaagct
     1141 ccccaagcac tattacgaaa gactggttag agtgaaactt tactgacaaa gtgattgttg
     1201 cttcatggag taacattaaa attaaaaatt ttttattatt ctaattatat tacgaatatt
     1261 acaaaataat agctatttta atagaattta taagttaaaa tattatgata aaaatcacaa
     1321 acgtagcctt ttacgtcata agtgttaaaa gaaataatta atttcaaata tagaaaaggg
     1381 aacgtatgtt tgcatactcc ttttttttga gttataataa tacgcgaaga gagataattt
     1441 cattatttaa acaagctaaa aatcttgata ttacttcttt tcaggcaata cgaatattat
     1501 tcgcttacat tttttgtata aatttaattg tctgctatgc ttgtgatgaa ttgattttcg
     1561 ttttgcactt ttggatgata aatttggaca attgaaaata caaaaaaaag aaaggcggta
     1621 tgaactcgga aggctggtaa ccttcgtcag cgttcataca cggcattaag atttggccct
     1681 taatgcgtta acctttcagt gttacggtat tacccgcaat ggatttagta taacagatat
     1741 tcaaaagtga taccactttt tttggctgtg ttaaggttaa aaatacgtga tattacgatg
     1801 aatctatgcc taagcataat gttataaaaa tgtccgtgtg gctctttgcc gtatgacgaa
     1861 cgctgaaatt agagggaacg catgtttgga tgcgttccct cttttttgtt tgtcatgaac
     1921 gaaaatcaat tctctatttt tgaaaaataa caagaaaaac ttgttaaaaa tatttaaaga
     1981 aattgggtgg tttattttat gtcatataca ggaattttga gtctcaaaga tatttgtcac
     2041 tacggtaaac ggtgtaccgc tacagagaaa atcacgaaga aactatcaac tggccaaagt
     2101 aaaaccgttg tgcagtgtaa gaaatatata atccagaaag acaaagtctc agaggaaatg
     2161 atttattaca ttgggaaaca aaagcaaata attttaaaag atcctatccc cttaaaagag
     2221 ctatatccaa caatcaaaca tgtttatgac caaaacggtg tactgattgg tcgaagaaaa
     2281 aatggagtct tgcgttgtac cgcaaaaggg atggggcgtt tgatcggctg aattgatcat
     2341 tctcataact tgtattttat cgtttttcgc ttgcttgaaa atattgtcga gacttctaaa
     2401 ggaacttaga gtcactagaa aaataaggag tagacttgat ctatcaacaa ttcccttcgg
     2461 ggagtgacgg ggtaggtttt aacgattggt aacgtaaaaa attggtcaat cgagtaaatg
     2521 cgcttgtttg gatagtggtg aaatgaattt gtgtaaataa taagcgcaga gtaaacaaga
     2581 gggaatttag aagttagaca agttagaata gcgtctagat taatcatgag cagctactaa
     2641 gggatctaga ctttgacgca aactcatgga ataaggagta ggtcttctcg taatggggag
     2701 gacgcacaag aaattgtgtc gtgtgctgca gtacatgtag atacttgcga ggacgttgat
     2761 tattggatca atcgagcatt taccgctgtc tactagggtt tctcgtttcg gacgccataa
     2821 acgagtcgaa attttttatg tatttttaag taaacgaaaa acgataaaat atgaacaagt
     2881 cctgaataaa gtgaaaaaat taatataaag catagagaaa atgaaattta cgtaatggag
     2941 gaatgacaaa tgaaaaatta tgatccgaat atacgattgg gcacacatac tattaaggtt
     3001 tctttccaac gttgggatta caaaggattt gtgacgttta gaaggggcgg aaattgtaaa
     3061 ggattagatg ttttagcatt agacgaggat gatttatacg atcaaacatt gactgataat
     3121 ccaattggat tcggcttgct acctgaagat gatgaaggta atgaatggtt taaaatgacg
     3181 cttatgaatg ataatggaga cgaattatct gttgaagata cttggtcata tttgagtgat
     3241 tatattgtca gctttgaaat cattgaattt gtagcggata aagaagaata aaaactggag
     3301 gaataaattt tacagataaa ggcttagaag gagtcacgac gattaataaa aaggtagtat
     3361 taccttaggc attgagtaag gaatttattt agtctaatgg tgataagtca agatgaaatt
     3421 taagattttc tttattgagt aatatgtttt gaagcgcacg aaactagacc cgatcaaaat
     3481 caaatttttt tcgggttgat tcaaggattt gcgtcaataa cggattgtta tttctctatc
     3541 acactccttg gtggcgtata gagttggtgt ttacgtagta gcgtatccac cagacgcgta
     3601 aattttcttg cggttaggac gagtgcacgt ttgtgttgat gctttggaac ctccttatat
     3661 ttactttgat aaaaagcttt gtattcagga agataatttt ttacagagtt ggcagcttca
     3721 actaagtagt atctaaaata tcgatttcct tgttttgtca gaggtgtatt ttgcgactga
     3781 tagtttccag attggttcac tttccaactt aatccagcgt attttgccaa ttttgtctga
     3841 tcttcaaatc tttcgatttg accaatttca gcgattaaac cggcagcata gactttacca
     3901 acgcctggaa tactggttaa gcattgatat tctggaatga caacaattac ttgttcgatg
     3961 gctttatcta attctttgat atttttttca agtgctcgta tttctcgtac gagaacactt
     4021 aaaacaacat taattgattc ttgtgcaaga gcacctaggc ggtaactcat ggtaattgca
     4081 cgttgaattg ctttagcgat tttttctggt tgcttgaaac ggccttttcc tttttcttga
     4141 agtaaatcac agaaagcttc taaaggaagc tcagcaagtt catccaatgt gaaatcttcc
     4201 gtcatgagtg aaatgagggt tgcactaaaa agactcgtcg attcgtctcg catttctcga
     4261 gcaagcgtat tacatttgta cgttagattt tcaagaaaat gttgctttgt acgaatcaat
     4321 tgcttaataa gttgatagcg agtacgtgtt aaacgctgta aggccatgta tttctcttct
     4381 ttgatagggg aagttgtgaa tcgttgaata cgcaagaaat cagcgattct taatgcatcg
     4441 tttcgatcag ttttattctc atcaaacatg cgactaaatt gtttcacccg aaaaggattt
     4501 tcaatcgtta caagtgtgtt gagcttcttt agtttctcat cttcatgaaa aaacatggaa
     4561 ggatggaagc tgtacatgga cgttgactcc atgccaatca caa
//
LOCUS       DAZKLM010000138         4500 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3764
            SAMEA115706913-rid22582563.denovo.144, whole genome shotgun
            sequence.
ACCESSION   DAZKLM010000138 DAZKLM010000000
VERSION     DAZKLM010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706913
            Sequence Read Archive: ERR13196647
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4500)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4500)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287823.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287823.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,004
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,849
            CDSs (with protein)               :: 2,849
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 41 of 96
            Pseudo Genes (incomplete)         :: 52 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 15 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4500
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706913-rid22582563.denovo.144"
                     /isolate="VRE3764"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(609..2114)
                     /locus_tag="ACG842_002627"
     CDS             complement(609..2114)
                     /locus_tag="ACG842_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFQ0249108.1"
                     /translation="MIKPKKSEKRELKGPSLTIKWAFASSFFIFVVFTIFAVITYKSS
                     INLIVAKERNNVERTISEVASRLANSDEELTLVNTYRNLTKATNDGDEVVDHTSLVEG
                     NLMKIDSFIAELGQPSMDLYVYDLDENLIFKTHENSRNLIQTTRKVPTIVTLEGKTGF
                     LSIQPIYSKGTREKIGYAQSFYELSSFYDIRNKLLLTLIVLEVVSLILSSILGFFLSS
                     YFLKPLKVLRDTMDTIRKDPQSDIHMPEIDTNDELADLAEIFNEMLDRMRLYIEQQEQ
                     FVEDVSHELRTPVAIIEGHLSLLNRWGKDDPEILEESLSASMQEISRMKSLVQEMLDL
                     SRAEQVDVHYANETTKGKEVTYQVYNNFKILYPDYVITLDDDLTKEVTLQIYRNHFEQ
                     LIIIILDNAVKYSTTRKEVHISISSTLNEFEIAIQDFGEGIPKEDLNKIFNRFYRVDN
                     ARARTKGGNGLGLSIAKQLVENYKGRILAESVVGQGTIFRIFIPIAKAEDK"
     gene            complement(2111..2797)
                     /locus_tag="ACG842_002628"
     CDS             complement(2111..2797)
                     /locus_tag="ACG842_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFQ0249109.1"
                     /translation="MSNILIIEDEKNLARFVELELKHEGYNAEVHYNGRTGLDAALNN
                     EWDAILLDLMLPELNGLEVCRRVRQVKNTPIIMMTARDSVIDRVSGLDHGADDYIVKP
                     FAIEELLARLRALLRRIDIEGDKNVAKQTTITYRDLTIEKENRVVRRGNEVIELTKRE
                     YELLLTLMENVNVVLARDVLLNKVWGYETEVETNVVDVYIRYLRNKIDVPGEESYIQT
                     VRGTGYVMRS"
     gene            complement(2990..4411)
                     /gene="gndA"
                     /locus_tag="ACG842_002629"
     CDS             complement(2990..4411)
                     /gene="gndA"
                     /locus_tag="ACG842_002629"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HFQ0249110.1"
                     /translation="MSKQQIGVVGMAVMGKNLALNIESRGYSVALFNRTGAKTTAVVE
                     EHPDKNFKATYTIEEFVESIEKPRRILLMVKAGPATDATIQELLPHLDKGDILIDGGN
                     TFFKDTMRRNEELANSGINFIGTGVSGGEEGALKGPSIMPGGQKEAYALVAPILEQIS
                     AKAEDGAPCVTYIGPNGAGHYVKMVHNGIEYGDMQLIAESYDLMKNILNLSVEEMADI
                     FKEWNQGELDSYLIEITADILTRKDDEGTGKPVVDVILDAAGNKGTGKWTSQSALDLG
                     VPLPLITESVFARYISAYKEERVQASKILSRTNDFEFTGDKKELVEKIREALYFSKIM
                     SYAQGFAQLRVASKEFDWDLPFGEIAKIWRAGCIIRARFLQKITDAYDKNPEIENLLL
                     DDYFVEITKKYQQSVRDVVALAVQAGVPVPTFSSAIAYFDSYRAERLPANIIQAQRDY
                     FGAHTYERVDKEGIFHYSWYHEE"
BASE COUNT         1257 a          931 c          740 g         1572 t
ORIGIN      
        1 ttgaattgaa tcaagacgcc ctacacattt ggtttgtctt actttgttca gttttcaaag
       61 gtctaatctt tttgttttaa caactcttta atcttatcag attcagaaga gaatgtcaac
      121 aacttctttg tttatttttt ctaaacgtct ttgccactca ttagcagcga cttgattatc
      181 ataccatctt tattgttatt tgtcaataac tttttcgaag tttttttcga taaaatcaag
      241 ctttattagc tatctcatcc aaacgacttc gttattataa catgacatca aaagtgcgtc
      301 aacactattt tttataattt ttagtgacac cttaaaggaa tcatttagaa caaataataa
      361 tatagcaatc tctttataaa attgcaatag aaaattccct atttttattt attattttta
      421 aacacgaata ttacagcatt ttattgtttt ttcgttcatc taaaagatac ttacatttaa
      481 ttaacggatg ttattctatt taattgttcg ctagtttttt tataatgaca tccctgaatt
      541 tctcacttgc ttgtattttg taccttattc tttgttggat atataaaaaa acaagtaaag
      601 cacatgcttt acttgtcttc tgcctttgcg ataggaataa agattctaaa aattgtccct
      661 tgccctacta cactttctgc taagatacgt cctttatagt tttcaaccag ttgtttagcg
      721 atagacaacc ccagaccatt tccacccttc gttctagctc ttgcattatc cactcgataa
      781 aaacgattga agatcttatt aaggtcttct ttgggtattc cttcgccaaa gtcctgaatc
      841 gcgatctcaa attcattcag agtggaagaa atagagatat ggacttcttt tcgtgtagtt
      901 gaatacttta ctgcgttatc caatatgata atgattaatt gttcaaagtg attacgataa
      961 atttgcaggg tcacttcttt tgtcaaatca tcatctaaag tgatcacata gtctggatat
     1021 aatatcttga aattattata aacttgatag gtgacttctt ttcctttagt tgtttcattc
     1081 gcataatgaa catctacttg ttctgctctt gataagtcta gcatctcttg aactaagctt
     1141 ttcatacggc tgatttcttg catgctggcg cttaatgatt cttctaatat ctcaggatca
     1201 tctttccccc aacgatttag cagactaaga tgcccttcaa taattgcaac aggtgtcctt
     1261 aactcatgag atacgtcttc cacgaattgc tcttgttgtt caatatacaa acgcatacgg
     1321 tcaagcatct cattgaatat ttcagctaaa tctgccaatt catcatttgt gtcgatttca
     1381 ggcatgtgga tatctgattg cggatcttta cgtatagtat ccattgtatc tctgagtacc
     1441 ttgagtggtt ttaagaaata agaagataaa aagaaaccta aaatactact taagatcaaa
     1501 gaaacaactt ctaaaacaat aagagtcaat aatagcttat ttcgaatatc atagaaagaa
     1561 gataattcat agaagctttg cgcatagccg atcttttctc ttgtaccttt agaatatata
     1621 ggctggatag ataaaaagcc agtttttcct tctagtgtaa cgatcgttgg aactttacgt
     1681 gtagtctgaa tcaggtttcg actgttttca tgggttttaa aaatcaagtt ctcgtccaaa
     1741 tcataaacat aaagatccat tgatggctgc cctagctcag ctataaagga atcgatcttc
     1801 attaagtttc cttcgaccag tgaagtatga tctacgacct catctccgtc atttgtcgct
     1861 ttcgtcagat tacgatacgt atttactaat gtcagttctt catccgagtt agccaaacga
     1921 gaagctactt ctgaaatcgt acgctctaca ttgtttcttt cttttgccac aatcagattg
     1981 atagatgatt tataggtaat gacagcgaaa attgtaaaca caacgaatat gaagaaagaa
     2041 ctcgcaaagg cccacttgat tgtcaaggag ggccctttca gttctctttt ttccgatttt
     2101 ttcggcttta tcacgagcgc atcacataac ctgttccgcg tacagtttgg atatagcttt
     2161 cctccccggg aacgtcaatt ttgttgcgaa gataacgaat atatacgtcc actacgtttg
     2221 tttcaacttc tgtttcgtat ccccacactt tatttagtaa aacgtcacgt gctaatacca
     2281 cgttcacgtt ttccattaaa gtaagcagca actcgtattc tctttttgtt agttcaatta
     2341 cttcatttcc gcgacgtacg acacggtttt ctttttcgat agtcaaatcg cgataagtaa
     2401 tagtagtttg tttagccaca tttttatcgc cttcaatatc gattcgacgc aacaatgcac
     2461 gaagacgtgc taataattct tcgatagcaa atggtttgac gatgtagtca tctgctccgt
     2521 ggtcaagtcc agatacacga tcgatcacag aatctcttgc agtcatcata atgattggtg
     2581 tatttttgac ttggcgtaca cgtctgcaga cttctaaacc gttcaattct gggagcatca
     2641 gatccaacaa tatggcatcc cactcattat ttaatgccgc atctaaacca gttcggccgt
     2701 tgtagtggac ttctgcgtta tacccttcat gcttcaactc tagctcgaca aatcgtgcca
     2761 agttcttttc atcttcaatg attagaatat tgctcattta ttattttatc ctttctattc
     2821 tactggcttt ttaaaaactc tatcattata ccgataatat gcttaaaaat ctatagtcat
     2881 tttatcatgg atagcaattt taaaaaagag ctgaagtcta ctcataacaa tcgacttcag
     2941 ctctttcgtc ctctttttat tttctcattt cctaacagcc gaaagactat tattcttcat
     3001 ggtaccatga gtaatggaag attccttctt tgtcgacacg ctcataggta tgtgcgccaa
     3061 agtaatcgcg ttgggcttgg attatatttg caggcagacg ttctgctctg tatgaatcga
     3121 agtaagcgat ggctgatgag aaagtcggta caggtacgcc agcttggaca gccaaggcaa
     3181 cgacgtctct gactgattgc tgatattttt tcgtgatttc aacgaagtaa tcatctagca
     3241 ataaattttc gatctcagga tttttgtcat aggcatccgt aattttttgc aagaatcgag
     3301 ctcggatgat acatccagcg cgccagattt ttgcgatttc accaaatggc aagtcccaat
     3361 caaattcttt tgaagcgaca cgtaattgag caaatccttg ggcataactc atgattttac
     3421 tgaaatacaa cgcttctcgg attttttcga ccaattcttt tttgtctccc gtgaactcaa
     3481 aatcgttcgt acgagacaaa atcttgcttg cttgtacgcg ttcttcttta taagcagaaa
     3541 tatagcgggc aaatacagat tcagtgatca atggaagtgg cacacctaag tcaagcgcgc
     3601 tttggcttgt ccatttacct gttcctttat tcccagctgc atcaaggatc acatctacta
     3661 ctggtttacc tgttccttca tcgtctttac gtgtcagaat gtcagcagtg atttctatca
     3721 aatagctgtc taattcgcct tgattccatt ctttaaagat atcagccatt tcttctactg
     3781 aaaggtttaa aatattcttc attaagtcat aagattcagc aatcagttgc atatctccat
     3841 actcaatacc gttgtgtacc attttcacat aatgtccggc tccatttggg ccaatatatg
     3901 tgacacatgg tgctccgtct tctgcttttg cagaaatttg ctctaagatt ggtgcaacta
     3961 gcgcataggc ttctttttgt ccaccaggca tgattgaagg tccttttaaa gctccttctt
     4021 caccaccgga aacgcctgta ccgataaagt tgatgccaga gttggctaac tcttcattac
     4081 gacgcatcgt atctttaaag aatgtatttc ctccgtcaat caaaatatcg cctttatcaa
     4141 ggtgaggaag taactcttgg attgttgcat ctgttgccgg tcctgctttg accattagca
     4201 agatccgacg tggtttctcg atagattcta caaattcttc gattgtatat gttgctttga
     4261 aatttttatc tgggtgttct tctactacag cggttgtttt agcccctgta cgattgaata
     4321 aagccactga atagccgcgg ctttctatgt tcaaggctag atttttaccc ataacggcca
     4381 taccgacaac gccgatttgt tgttttgaca tatgaactcc tcctacatac gggatttctc
     4441 cctaatctct tacattatag cttaacacgg ctacattgca aactaaagaa caacatattt
//
LOCUS       DAZKLM010000139         4425 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3764
            SAMEA115706913-rid22582563.denovo.145, whole genome shotgun
            sequence.
ACCESSION   DAZKLM010000139 DAZKLM010000000
VERSION     DAZKLM010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706913
            Sequence Read Archive: ERR13196647
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4425)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4425)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287823.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287823.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,004
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,849
            CDSs (with protein)               :: 2,849
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 41 of 96
            Pseudo Genes (incomplete)         :: 52 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 15 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4425
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706913-rid22582563.denovo.145"
                     /isolate="VRE3764"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            690..1658
                     /locus_tag="ACG842_002630"
     CDS             690..1658
                     /locus_tag="ACG842_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFQ0249111.1"
                     /translation="MDVAKTVNAIVEKNDGEISGIYLVACGGSLVDMYVSDYFLKAEA
                     SKCITGLYTANEFNHVTPKQLDDKSVVILCSHGGNTAETVKAGEVAQARGAVTVGLTH
                     NKTADLLKVSDHSFLYEWGDDAKIVNNPMAIILDLTVSLVNVTETFEHYGKFREGMKV
                     IDTVIENGIKQVQDRIKVFADKYKDETMFYILGSGPSYGHAYGFSICSLMEMQWLDST
                     SVHSGEFFHGPFEVTDKDTNFILLMSIGRTRALDERAKRFLDKYAERVEIVDAKELGL
                     DMISDEVSEFFNPILFYSILSEYRSALADVRNHDLDVRRYMGKVEY"
     gene            1669..2484
                     /locus_tag="ACG842_002631"
     CDS             1669..2484
                     /locus_tag="ACG842_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-kinase"
                     /protein_id="HFQ0249112.1"
                     /translation="MKVLGLGDNVVDKYENLGIMYPGGNSLNFAVFAKKLGHESSFMG
                     VFGSDEEAAHVLSAIKEIGLDNSHSRFEKGENGCARVKIDEGDRIFLGSNSGGVTREY
                     PIQLTEEDREYLNQFELIHMGLYSHVNHLLEELQNVSGKISYDFSDDFTEEEVQRAID
                     KVDFGFFSIEDFSDEEVKQFLMKHFCERNDVLIATRGEKTAIGYDGAKFYEVNPVLEE
                     PVDTMAAGDSFLTAFLLYFLEGDDIVTAMKKGNEFAGKSCLVDGSFGFGIHYD"
     gene            2552..3262
                     /locus_tag="ACG842_002632"
     CDS             2552..3262
                     /locus_tag="ACG842_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFQ0249113.1"
                     /translation="MLDTNAQLPLYLQLKEILKNKINTGEYPENTKIPAESELIESYE
                     VSRITVRKAVEELVEEGYLLKKQGKGTFVNRHKVFRKIEYIMGFSESCKSNGFTPTSI
                     VLERKIIPATEGMAKKLDIAQGDDVLYIQRKRMADNIPIMLENNYFSYERYSFLMEEE
                     LTDSLYEVLKVQGTLAINPGETALEIVTADDQIAKIMEVAVGTPFFFMDTLINDQNNI
                     PIHVGNQYYLGEVYRFYL"
     gene            3298..>4425
                     /locus_tag="ACG842_002633"
     CDS             3298..>4425
                     /locus_tag="ACG842_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HFQ0249114.1"
                     /translation="MKQILRSKMIFDGEQPPFKGYIVINEGIIEKVENNWNYSDLLNE
                     NTELIAYDDQFVMPAIHDNHVFFSGYLAMNAGIDLSDTSSANEAVERIKAVMNHKKAD
                     EPIYAHGWDAALWKTDPSEELLNEINHFGPITAIDKNRSHCWMNAIAKQTYGFTEKET
                     SAESRVLLIKEMLANKKLLAEVYAEFEALLLSKGVISMKEIVFDDAEFLSNIPQRKIL
                     SNLYVQAVQEPLQHELLYEYKEKVFPEKVRFGGVKIMVDGVVADRSGDIYGVYQSGVA
                     SPEIDYEAIKAEVAAFNEVEIPCCLTTEGDKAGYKAASILVEFGKRLPEGVFNSISDL
                     EMVTQKTAEKMNEGQVVAEVYPQILGLNQTLEEAYMSTVIKN"
BASE COUNT         1490 a          661 c          921 g         1353 t
ORIGIN      
        1 atacaaataa ttcaccaaaa aggtgagtaa tgccattttt gaaaattgag cttgttttat
       61 gtctacatct gtatccgtat ataatccaaa ggattataaa ctcagaagag tctttatttt
      121 ttctgaatat aactactgta tcaaaaacaa tcgattatga gacaaaacaa aatgacctct
      181 tttatttaag aaaggaggtc attttttgta tcataaattg tattaaaatc aatgtatcac
      241 tttttaatca gttttaagac cataaattgc gtactaaaag aaattctgca attcaaaata
      301 aagcatttta agccattttc aaatttaagt gcacccatct acccgtcaaa tcactttatt
      361 ttccactcca gtagtttcct aattcaactg tgtgcaaaaa cgatttcttc taaacaaatc
      421 aagacaaact tataaacctt catcccagca ccataattat ttaaatcata ggcatttttc
      481 gaaattagta tatgtactca agtttcactg cagattattt atatttttta gagaaataaa
      541 tttttcctat tgacaaacac aaagaaagcg gatacaatgc aattatgtca catgacgtta
      601 tatgacgtta tgtgatatct ttacatttaa tcagttactt ttattttgga taaaattaac
      661 atttttaaaa ttttaaggag gaaatgttga tggatgtagc aaaaacggtt aacgcaattg
      721 tagaaaaaaa tgatggagag atttctggaa tttatttggt ggcttgtggc ggttcgctgg
      781 tagatatgta tgtctcagat tattttctta aagccgaagc atcgaagtgt attacaggct
      841 tatatacagc gaacgaattt aaccatgtaa ccccaaagca attagatgat aaatctgttg
      901 tcattctatg ttctcatgga ggaaatacag cagaaacggt aaaagcagga gaagtcgctc
      961 aagctagagg agctgtgact gttggtctga ctcataataa aactgcggat ttattaaagg
     1021 tttctgatca tagtttcctt tacgaatggg gagatgatgc aaaaatagtc aataatccta
     1081 tggccatcat tcttgattta actgtatctt tggtcaatgt taccgaaaca tttgaacatt
     1141 acggtaaatt cagagaaggc atgaaggtta tcgataccgt aattgaaaat ggcattaagc
     1201 aggtgcagga tcgtattaaa gtgtttgctg ataagtacaa agatgaaaca atgttttata
     1261 tcttaggtag tggaccatca tatggacatg cttatggttt ttcaatttgc tctttgatgg
     1321 aaatgcaatg gcttgattca acttctgttc attctggaga attcttccat ggaccttttg
     1381 aagtaacaga caaagatacg aattttattc tcttgatgag cattggacgc actcgtgctt
     1441 tagatgaacg ggcaaaacga ttccttgata aatatgcaga aagagtagaa atcgttgatg
     1501 caaaggaact aggattggat atgatatcag atgaagtttc tgaattcttc aatcccattt
     1561 tattctacag tattctttca gaatatcgtt cagctttagc agatgtacgc aatcatgatt
     1621 tagatgttcg tcgctatatg gggaaagtgg agtactagga ggattaaaat gaaggtatta
     1681 ggcttaggtg ataacgtcgt cgataaatat gaaaatctgg gaattatgta tcctggagga
     1741 aattcattga attttgcagt ttttgcaaaa aaactgggac atgaaagtag ctttatggga
     1801 gtttttggct cagatgagga ggcagctcat gttttatcag caataaaaga aataggactg
     1861 gacaacagtc actctcgctt tgaaaaaggc gagaatggct gtgccagagt gaagattgat
     1921 gagggcgacc ggattttcct tggcagcaat tcgggaggtg ttactcgtga atacccgatt
     1981 cagcttactg aagaagacag agaatattta aaccagtttg aattgattca tatgggatta
     2041 tatagccatg tcaatcatct attagaagag ttacaaaatg tttctggaaa gatttcttat
     2101 gatttttctg acgactttac ggaagaagag gttcagcggg caattgataa agttgatttt
     2161 ggcttctttt ctatagaaga cttttccgat gaagaagtaa agcagttttt gatgaagcat
     2221 ttttgtgagc gaaatgatgt attgattgca acccgaggag aaaaaacggc tattggttat
     2281 gatggagcaa agttttatga agtgaacccc gtgctagaag aaccagttga tacaatggca
     2341 gcgggagatt ctttcttaac agctttccta ctttattttc tcgaagggga cgatatagtc
     2401 accgcgatga aaaaggggaa tgaatttgct gggaagtctt gtctggttga cggttcgttc
     2461 ggctttggta ttcattacga ttgagcttgt tttaaattcc tgcatttgat aacctaaaga
     2521 aagaaatccc ggtaaagtgg aggttaaaaa aatgctggat acaaatgcac aattaccatt
     2581 gtacttacaa ttaaaagaaa tattaaaaaa taaaatcaat acaggggagt atcccgagaa
     2641 tacgaaaatt cccgctgaat cagagctgat agaaagttat gaggtcagcc gaatcacagt
     2701 gagaaaagca gtagaagaac tggtagaaga agggtaccta cttaaaaaac aagggaaagg
     2761 aacttttgtc aaccggcata aagtttttcg taaaattgaa tatattatgg gtttcagtga
     2821 gagttgtaaa tctaatggtt ttacaccgac tagcattgtt ttggaacgca agatcattcc
     2881 agcaactgaa gggatggcaa aaaaactcga tatcgctcaa ggtgatgatg ttttatatat
     2941 ccagagaaag cgaatggcgg ataacatccc cattatgtta gaaaacaatt atttttctta
     3001 tgaaagatat agtttcttaa tggaggaaga gctgactgat tctttgtatg aagtacttaa
     3061 agtccaaggt accttagcca tcaatcctgg ggaaactgca cttgaaattg tcacagctga
     3121 tgaccaaatt gccaaaataa tggaagtagc tgttgggacg ccctttttct ttatggatac
     3181 tttaatcaac gatcaaaaca acataccaat tcatgtaggt aatcaatatt acctgggaga
     3241 agtatatcgt ttttacttat gatttcctgt ttggatttca aggagtagat tttatttatg
     3301 aagcaaatat tgcggtcaaa aatgattttt gatggagagc agccaccttt caaaggttat
     3361 attgtaatca atgaagggat cattgaaaag gtggaaaaca actggaatta cagtgatcta
     3421 ttaaatgaaa acacggaatt aattgcttat gatgatcaat tcgttatgcc agcaattcat
     3481 gataatcatg tatttttcag tggttatttg gctatgaatg ctggcataga tttaagcgat
     3541 acatcttcag ctaacgaggc tgtagagcgt attaaagctg taatgaacca taaaaaagca
     3601 gatgaaccaa tttatgctca tggctgggat gctgccctgt ggaaaactga cccatcagaa
     3661 gagctattga atgaaataaa ccactttggt ccaatcacag cgatcgacaa aaatcgcagc
     3721 cattgttgga tgaatgctat tgctaagcag acttatggtt ttacagaaaa agaaaccagt
     3781 gctgaaagtc gagtattatt aattaaggaa atgctagcaa acaaaaagct tttagcagag
     3841 gtttacgctg aatttgaagc attactgctt tcaaaaggtg ttatatcaat gaaagaaatc
     3901 gtttttgatg acgcagaatt tctttctaat atacctcaga ggaaaatact tagtaaccta
     3961 tatgtgcagg cagtccaaga acctctacag catgaacttc tatatgagta taaggaaaaa
     4021 gttttccctg aaaaggtccg ctttggtgga gtgaagatca tggtagatgg tgttgttgcg
     4081 gatagaagtg gagatattta cggtgtttat cagtcaggag tagcttcacc cgagattgac
     4141 tatgaggcaa ttaaagcaga agttgctgca ttcaatgaag tagagattcc ttgctgtttg
     4201 acaacggaag gagataaagc gggttataaa gctgcgtcaa ttcttgtcga atttggaaag
     4261 cggttacctg aaggcgtttt taattcgatt agtgatttgg aaatggttac tcaaaagaca
     4321 gcagaaaaaa tgaacgaagg acaagtagta gcagaggtat atccacaaat tctcggcttg
     4381 aatcaaacgt tagaagaggc ttacatgtca actgttataa aaaat
//
LOCUS       DAZKLM010000140         4420 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3764
            SAMEA115706913-rid22582563.denovo.146, whole genome shotgun
            sequence.
ACCESSION   DAZKLM010000140 DAZKLM010000000
VERSION     DAZKLM010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706913
            Sequence Read Archive: ERR13196647
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4420)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4420)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287823.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287823.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,004
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,849
            CDSs (with protein)               :: 2,849
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 41 of 96
            Pseudo Genes (incomplete)         :: 52 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 15 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4420
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706913-rid22582563.denovo.146"
                     /isolate="VRE3764"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(334..735)
                     /locus_tag="ACG842_002634"
                     /pseudo
     CDS             complement(334..735)
                     /locus_tag="ACG842_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377045.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TetR-like C-terminal domain-containing protein"
     gene            complement(924..2243)
                     /locus_tag="ACG842_002635"
     CDS             complement(924..2243)
                     /locus_tag="ACG842_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1380-like element IS1678 family transposase"
                     /protein_id="HFQ0249115.1"
                     /translation="MTSLHKNQVKFNSNITISHTGGQLSSDSGLVLVKELMNIFGFSE
                     LAKQHIHIEDERAYFTHDNLSILEQFIMQLIAGYSADSAANLLRQDPVFKAVLDRKEL
                     ASQSSLSRFLDRLSEENIHELQALNQELIDKARLIRNDTELIIDLDSTHSDTFGHQEQ
                     TNYNTHYQTYGYHPLVAFDGLTGDFLKAELRSGNQYTSKGVKEFLTPLLEHYNHSLPN
                     TDILVRGDSGFATPGVYDSCESKKSHYVIRLKNNRRLGQIAEKSVLYGDNQKWEEREV
                     QYFSTTYQAQSWSQSRRVCIRSTREAGELLFRHEFIVTNLSENVSPDTIFSIYAKRGT
                     MENFIKEAKAGFYFDKTDSPRFLENHVRMMLSLIAYNLVNFLRTIGFEEVQKGMTIHS
                     IRLKFLKVAGKLVQTGRRVYLKLSSYHVYQNEFYRVFARLRRASQWI"
     gene            2500..2970
                     /locus_tag="ACG842_002636"
     CDS             2500..2970
                     /locus_tag="ACG842_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4809 family protein"
                     /protein_id="HFQ0249116.1"
                     /translation="MKEVQLIRKSELSEGGCNACGVVEATSYTLKLGANKAIISELTV
                     GGLVDSLALAEGFIGEDIYEMFSEIRQLKKGENCIEVHHESPNVRFKRGDNEMIFNNH
                     VSDHTELYGIVNQILTELFGLGPYAFKEENGNPKLNEEWQETIEIQRNNPHLFQ"
     gene            3192..4295
                     /locus_tag="ACG842_002637"
     CDS             3192..4295
                     /locus_tag="ACG842_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HFQ0249117.1"
                     /translation="MEKTYQAGTNEGIVDFINMEDLEIAASQVIPAGGYGYISSGAGD
                     LFTYQENERAFNHQLIIPHVLRDVELPDTTTHFDKETLTAPIIMAPVAAHGLAHVKAE
                     KASAKGVADFGTIYTASSYASCTLEEIREAGGEKAPQWFQFYMSKDDGINLDILEVAK
                     RNGAKAIVLTADATVGGNRETDRRNGFTFPLPMPIVQAYQSGVGQTMDAVYKSSKQKL
                     SPKDVEFIAAHSDLPVYVKGVQSEEDVYRSLESGAGGIWVSNHGGRQLDGGPAAFDSL
                     QYVAEAVDKRVPIVFDSGVRRGQHVFKAIASGADLVAIGRPVIYGLSLGGSTGVHQVF
                     DFFKTELEMVMQLAGTQTVEDIKKIKLRENRFI"
BASE COUNT         1435 a          736 c          916 g         1333 t
ORIGIN      
        1 tgattcccct tgaaaatgaa ttgaggtaaa cgagaagaat tcaataaact ggatgataaa
       61 aatataatat gactataaat tggttacagc ttcaacctat aagttgtaat aaattagcct
      121 aaaagtaatt tattaatgat gaggtgaatt tgattggtac aacaagatat atcacttaga
      181 gagggagaag cagcactaca aactactttt caataagtag gaattataga aattttactc
      241 aaaaaatctt cagtaaacaa tttggctaaa taagattcag ttgttatacg agaaggttac
      301 ggttcttctt aactaagctt agtatgtttt tattcaaggc atgattttct ctatgtgatc
      361 attcagtttc tgtaagagtt ctaataattc ttttttggag tacttgtaat tgctttctac
      421 ccaccgattg agaacggata gacaaccttg gacgtggtac gtagtaaaga atctcatttc
      481 ttctgtgata atcgttttct tgtcttcata tagccatatg ttcagatagt tttcttcaat
      541 aacttgttta actttcttct gaaacgaaat atcaccatgg ttgaggataa ataagcgata
      601 taaaaaagta tttgcgtaaa catagtcaat caagcgagtg tatagttcct catagctatg
      661 ggctggatca taagagagaa tttcatttag atcccgaaaa aagttgcttt ctaattgatc
      721 cctgaataat tcatagcttt aattctttgg cacattttat tgaaatatgt atcactaata
      781 tccctatcaa ctgattttga ctaaaaagtt acttcaaaca ttcactgttc aaaaatattg
      841 aggaataaat tttgggtgta aggcattttg agcatacttc tccctggata acttcccagt
      901 aaaaaaatcg ttagattgtt ggattagatc cattgactgg ctcgcctcag gcgagcaaag
      961 accctgtaga attcattctg atacacatga taactagata atttgagata gactcgtcta
     1021 cccgtttgga ctaatttccc agcaactttc agaaacttca atcgaataga atgaatggtc
     1081 attccctttt ggacttcttc aaagccaatc gttcttaaga agttgactaa gttgtaagct
     1141 atcaagctga gcatcattcg gacatgattc tccaaaaagc gaggactgtc tgttttgtca
     1201 aagtaaaagc cagctttcgc ttctttaatg aagttctcca ttgtgccacg tttggcatag
     1261 atagaaaaga tggtatcagg agaaacattt tctgatagat tcgtcacgat aaattcatgt
     1321 cgaaagagta gttcgcccgc ttcacgtgtt gagcgtatac acacgcgacg actttgtgac
     1381 caggattgtg cttgataagt ggtcgagaag tactgaactt ctcgttcttc ccacttttga
     1441 ttatcgccat aaagtactga tttctcagct atttgaccta gtctacgatt attcttcagt
     1501 cgaataacat aatgactctt ttttgattca cacgaatcat acacaccagg tgtagcgaac
     1561 ccgctgtctc cacgaaccaa gatgtcagtg tttggtagag agtgattata gtgctctaat
     1621 aaaggtgtga gaaactcctt tacgcctttt gatgtatatt gatttcctga acgtagttca
     1681 gcttttaaga agtcaccagt caatccgtca aaagctacca atggatggta accataggtt
     1741 tggtagtgtg tattataatt cgtttgttct tggtgaccaa atgtatctga atgggtcgaa
     1801 tctaaatcaa tgattaattc cgtatcatta cggatgaggc gtgctttatc gataagttct
     1861 tggttcaaag cttgaagttc atggatattc tcctcagata gtcgatctaa aaatcgggaa
     1921 agtgaagatt gagaagcgag ttctttccta tctaaaacag ctttaaacac aggatcttgt
     1981 ctcaggagat tagctgcaga atcagctgag taaccagcaa ttaattgcat aatgaactgc
     2041 tcaagtatgg ataagttgtc atgagtaaaa tacgctcgtt cgtcttcaat gtgaatgtgt
     2101 tgcttagcca gttcagaaaa accaaagatg ttcatcagct ctttcactag gacgagaccc
     2161 gaatcactcg ataattgacc acctgtatga gaaatagtga tatttgaatt gaattttact
     2221 tggtttttgt gtaagctagt catgaaaaga actcctttct tttggttggt ttagtcactt
     2281 taaccatagc agaaaggggt tcttttttca tcacttaacg ggtgaagatg aaaaagtaat
     2341 tgtaagctgc cgtgaggcag tttcacatgg ttttaattaa aagtatgaat tattcaggta
     2401 gaatctctga agaataaatc acgtgatcgt gacgataaaa tacacttacg tcaggtgata
     2461 aaaaatacat aagtagaaaa ctaagaggag gaacagaaaa tgaaagaagt tcaattgatc
     2521 agaaaaagcg aactatcaga aggtggttgc aatgcatgcg gcgtagtaga agcaacaagc
     2581 tatacattaa agcttggtgc caacaaagcg ataatctctg agttgacagt aggcggtctt
     2641 gtggacagtc tggcgcttgc tgaaggtttt attggagaag atatttatga gatgttttca
     2701 gaaatccgtc agttaaaaaa aggggaaaac tgcatagagg ttcatcatga gagcccgaat
     2761 gtgagattca aacgtggtga taatgagatg atcttcaata atcatgtgtc cgatcacacc
     2821 gagctatatg gaatcgtgaa tcaaatacta actgagcttt ttggattggg accttatgcg
     2881 ttcaaggaag aaaacggtaa tccaaaatta aacgaagaat ggcaagaaac gattgaaata
     2941 caaagaaaca acccgcactt atttcaatag gcatccacag tatttcgtaa aagggacttt
     3001 gacacgactt ttgtcaaaat cccatcgttt tttatctatt tgtgttctaa acatttcttt
     3061 cagagtagta acaactggaa tgactgaaat gtaaacagat tatttgcctg tatcaatacg
     3121 gaagagtaat ataaagtgtg taaacgctat catattattc attcaatttt ttatagaggg
     3181 aaggcattga aatggaaaaa acgtatcaag caggtacaaa tgaaggaata gtggatttta
     3241 tcaatatgga agatttagaa atagccgctt cacaagtgat tccagctggt ggttatggtt
     3301 acatcagcag tggtgctggc gacttattta cttatcaaga aaatgaacgg gcattcaatc
     3361 atcagctgat tattcctcat gtattaagag atgtagaatt acccgataca actacacatt
     3421 ttgacaaaga aacgctgact gcaccaatca tcatggcacc agttgctgct catggcttag
     3481 ctcatgtgaa ggcggagaaa gcttctgcaa aaggtgtagc agattttggg acgatctata
     3541 cagcaagttc ttacgcttct tgtacattag aagagataag agaagcaggc ggggagaaag
     3601 caccgcaatg gtttcaattt tatatgagca aagatgatgg gatcaatttg gatattcttg
     3661 aagtggccaa gcgtaatgga gcaaaagcaa tcgtattgac agcggatgca acagttggcg
     3721 gaaaccgtga aacagaccgc agaaatgggt ttaccttccc actgccaatg ccgatcgttc
     3781 aggcttatca atcaggagta ggccaaacga tggatgctgt gtacaaatct tccaaacaga
     3841 agcttagtcc aaaagatgta gaatttattg ctgctcattc tgatttgcca gtctatgtga
     3901 aaggtgtcca gtcagaagaa gatgtctatc gttcattaga atctggtgct ggcggaatct
     3961 gggtatctaa tcatggtggc cgtcaactag atggtggtcc agcagcgttt gattcgttgc
     4021 aatatgtggc agaagcagta gataaacgtg taccaatcgt gtttgacagc ggtgtacgac
     4081 gcggacagca tgttttcaaa gcaatcgcat caggtgcaga tcttgttgct attggcagac
     4141 ccgttattta tggattgtct ttaggaggaa gtacaggagt ccatcaagtg tttgatttct
     4201 ttaaaacaga attggaaatg gtcatgcaat tagctggaac acaaacagta gaagatatca
     4261 aaaaaataaa attgcgtgag aatcgtttta tttaagaaac aaatgacgaa aaaaatagga
     4321 gtgcggcaga tgatgccgta ctcctatttt tatagttcaa ggatgtcact aatagaataa
     4381 tttgaaacga aaaaataaaa ttgaaatatt ttttcatact
//
LOCUS       DAZKLM010000141         4389 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3764
            SAMEA115706913-rid22582563.denovo.147, whole genome shotgun
            sequence.
ACCESSION   DAZKLM010000141 DAZKLM010000000
VERSION     DAZKLM010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706913
            Sequence Read Archive: ERR13196647
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4389)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4389)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287823.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287823.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,004
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,849
            CDSs (with protein)               :: 2,849
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 41 of 96
            Pseudo Genes (incomplete)         :: 52 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 15 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4389
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706913-rid22582563.denovo.147"
                     /isolate="VRE3764"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            510..656
                     /locus_tag="ACG842_002638"
                     /pseudo
     CDS             510..656
                     /locus_tag="ACG842_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332517.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamate
                     endopeptidase"
     gene            complement(744..1037)
                     /locus_tag="ACG842_002639"
     CDS             complement(744..1037)
                     /locus_tag="ACG842_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2087 domain-containing protein"
                     /protein_id="HFQ0249118.1"
                     /translation="MVYTYTEQEKMKVLDTFMNDGKVKQVPSSTKKKYILLKEVLKRF
                     DHGVTYTETEVNSILLNVFSSGDYVEQRRYLITFGFFKRSSDGRAYQMMGIEN"
     gene            1074..1259
                     /locus_tag="ACG842_002640"
     CDS             1074..1259
                     /locus_tag="ACG842_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0249119.1"
                     /translation="MLEYSGFFIKAVTLKKDIIIVLFFLLLLIGRFILIHYSPLPIIK
                     EKTGSGCSNYMLRKKNP"
     gene            complement(1440..2618)
                     /locus_tag="ACG842_002641"
     CDS             complement(1440..2618)
                     /locus_tag="ACG842_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFQ0249120.1"
                     /translation="MEKTDRFKKQISSTGRITWYFQAYRTTRRGFNSKREAQLAYLEM
                     EKQQRLKKKIVASNDKFSVVGEQWFQYYRSLNEQKEITYDKREEQLKIIVRWIGDKKL
                     SDLTPECLQELLFTLKECGVNGTNVGYAKNSLQSLIQVLNMIFKYCQRKNLISENPMK
                     SVRMPKYQLTVTDLKEAINNIEDKFLTVDELRTFLNYGIVHEELPMSVLFHVLFYTGC
                     RVSEALALQPGDIDFKRNEILFYKQTAVKGKSKDFRIETTKTVSSARRVLVTPLVMEK
                     LQQLIKALGEMRQHYRFIIEETYLFVYLDSSKRGVPYRREYVNDHVKRCVNRSGINKP
                     FHTHLARHTMASLVAEHCSWEVLKDRLGHTDQTTSKIYRHLTSNEKVKPLIAFSSLES
                     "
     gene            complement(2722..3036)
                     /locus_tag="ACG842_002642"
     CDS             complement(2722..3036)
                     /locus_tag="ACG842_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0249121.1"
                     /translation="MELEVKTNVNSLEIEFYSILQKKLEDYTEKLLAVSHLPLVLTNT
                     DLKRLFQISDSTLNRLVKLGEFPPCWYGIRGHYFRDDVLEWMRKSDSNDYREKLRLLR
                     SL"
     gene            3143..3358
                     /locus_tag="ACG842_002643"
     CDS             3143..3358
                     /locus_tag="ACG842_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0249122.1"
                     /translation="MKQGIADIKIIKEILEKSTANAIAFGTGINLSTVKKLKSGERAE
                     EKLNLADAIKITEFGMKNMPTKIEIWK"
     gene            complement(3658..3900)
                     /locus_tag="ACG842_002644"
     CDS             complement(3658..3900)
                     /locus_tag="ACG842_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0249123.1"
                     /translation="MKQNKRYIPFIPLTTIKAAASGDSIAMSIVLSKYQNYISRLAMK
                     PVYEEEKGMVMYVDEYMRRQLETKLIEKILEFDTTR"
     gene            complement(3887..4315)
                     /locus_tag="ACG842_002645"
     CDS             complement(3887..4315)
                     /locus_tag="ACG842_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HFQ0249124.1"
                     /translation="MGDSEETHIQHTFDSFCKKVVCNEALNIQKKYARFRQRQISMEI
                     LEQKGLGTEFYYLEPNLNSSGTFIVLGMKIFISNEELANAIAALPSQRQEIIFMSFFI
                     GLNDREIGDIIGKSLGSVWYQRQMALEELGKYLGVKYEAK"
BASE COUNT         1388 a          834 c          654 g         1513 t
ORIGIN      
        1 tctcttttta ttctaaggtt attttagtct tgtcctttaa tacaaagatt ttatttttaa
       61 ataaaaaata ctcttttttt acgattattg aaaaaaaaag ataaaacttg attaattgaa
      121 gtattgctta aatttggaag ctatgaggga agaataaaag agttaggacg ggattatcta
      181 ctatttttct ttgttttaaa tatttgttat ctttgaacat aaaagagaaa tgtgtcgact
      241 gcagtgttgt gtccctcatc acgaataaac ggtgttcaac tagctgatct tgtattcact
      301 taaaaaatgg taaagtacga gttgctattc cccattttca attttctaac gctcaggaca
      361 aaatactgtt ttcacaacct cattggaata aaagcatata tattgtttca agaacgaaat
      421 cttgttatct ttttaaaggg catctgtcca aaatctaatt ttaggaggta cgttttggta
      481 tttattcgta caaataaaag agaattatct ttattcatgt aacgcatcca attgattttt
      541 tcatgtacgc tcctggaacg attttactga cgattgctta tttaactgcg aatcgctcat
      601 tagcttttat catagctatc catattttga ataacgttct tggctttgta ttatagatac
      661 agatgacaaa aaaagttcaa gtatgcggtg tagaaccaat atcgcatact tgaacttttt
      721 tcaatgagta taaattaaaa cttctaattt tcaattccca tcatctgata tgcccgaccg
      781 tctgaacttc ttttgaaaaa gccaaatgtg attagataac gtctttgttc gacataatcg
      841 ccactagaaa atacgttcag cagtatcgaa ttaacctctg tttctgtata agtcactcca
      901 tggtcaaagc gtttcaagac ttctttcaat aggatgtatt tcttttttgt tgaacttggt
      961 acttgtttta cttttccatc attcatgaat gtgtctaaaa ctttcatctt ttcttgctcc
     1021 gtataagtat aaaccatctt ctttctcctt ttgattctaa gaatactcca tttatgttag
     1081 aatattctgg attttttatt aaagcggtta ctcttaagaa agatattatc atagtgctct
     1141 tttttttact actacttata ggtagattta ttctaatcca ttattctccc ctaccaataa
     1201 ttaaagaaaa aactggatca gggtgttcaa attacatgct aagaaaaaag aacccttgaa
     1261 taccaagggt tctttctcag tctgataaca atgattaacg acggatttct ttgatacttg
     1321 tgaaaagtcg ttaaagtatt gatataaaag gatttttatt acctctgact acatattgac
     1381 taatttgtta aaaaatcgtt tttcaagaat cctgtaatac cagtgctttc accgtttttt
     1441 taactctcta aagaactaaa agcaatcaga ggttttacct tttcatttga cgttaagtgg
     1501 cgataaattt tcgaagttgt ttggtcagta tggcctaagc gatctttcaa gacttcccag
     1561 ctacagtgtt ccgctactaa gcttgccatt gtatgacgag ctaaatgagt atgaaatggc
     1621 ttattaattc ctgagcgatt cacgcaccgt ttgacgtgat catttacata ctctcgacga
     1681 taaggaacac cacgtttact agaatcaaga tacacaaaca aatatgtttc ttctataata
     1741 aacctgtaat gttgcctcat ctcacccaaa gccttaatca actgttgtag cttttccata
     1801 actagaggag ttactagaac tcgtctagca gaactaactg tcttagtagt ttcaattcga
     1861 aaatccttac tcttaccttt aactgccgtt tgcttataga acagaatttc atttcttttg
     1921 aaatctatat ctccaggctg tagagcaagc gcctcgctga ctcggcagcc agtgtaaaat
     1981 agaacatgga aaagtacaga catcggtaat tcctcatgaa caatgccata attcaaaaaa
     2041 gttctcaatt catcgacagt aagaaacttg tcctcaatat tatttatggc ttcttttaaa
     2101 tcagttaccg ttaactgata ttttggcatt ctcacagact tcattggatt ttcacttatc
     2161 aaattctttc tttgacaata tttaaaaatc atatttagaa cttgaataag tgattgtaaa
     2221 ctatttttgg catagccaac atttgttcca ttaacgccac attcctttaa ggtaaaaagc
     2281 aattcttgaa ggcactctgg tgtcaaatca gacaactttt tatcaccaat ccatcgaaca
     2341 ataattttta attgttcctc tcgcttatca taggttattt ccttttgttc atttaacgaa
     2401 cggtaatatt ggaaccattg ctcacctaca acactaaact tgtcattgct tgcaacaatt
     2461 tttttcttaa gcctttgttg tttctccatt tccagataag cgagttgcgc ctcacgtttt
     2521 gaattaaagc cacgtcttgt tgttcgatat gcttgaaagt accatgtgat cctaccagta
     2581 gaactgattt gcttcttgaa tcgatctgtt ttttccattt attgattcct cctattttga
     2641 aggaacccaa caagcagaaa ccctttggaa atctttctat ctatgtcatc attgtaccgc
     2701 atcttagaaa gaaaatgtaa cttataaaga tcgaagtagt cgcaattttt ctcgatagtc
     2761 attcgaatca ctctttctca tccattctaa aacatcatca cgaaaatagt gtcctcgaat
     2821 cccgtaccaa cagggaggaa actcaccaag ttttactaaa cggttcagtg tactatcact
     2881 tatctgaaat agtcgcttta aatcagtatt tgttaatacc agaggtaaat gactgactgc
     2941 aagcaatttt tccgtgtaat cttccagttt tttttgtaaa attgaataga actctatttc
     3001 cagagaatta acatttgttt taacttccaa ctccatcacc cttctctctc aaaatcaaaa
     3061 cgaccaccac ggtacccctt aaaattgtaa aaaaacactc cagtactcca tcaaaacgga
     3121 ctagaaaaga ggaattttta atatgaaaca aggaattgca gacataaaaa taattaaaga
     3181 aattcttgaa aaatctacgg ctaatgcaat agcattcgga acaggaatca acttgagtac
     3241 agtaaaaaaa cttaaaagtg gagagagagc tgaagaaaaa ttaaatcttg ccgatgctat
     3301 taaaatcaca gaatttggca tgaaaaatat gccaaccaaa atcgaaatat ggaaatagta
     3361 gtttttctga tctatcagct gttttgccaa agcacatagg aatctcacct ccccccagta
     3421 atggcgggcc gatttggtta cggaagtatc attatcgtag tcgcgattct catattaaaa
     3481 tttgtcgctt aaaagctaac cttgtcatgg cgtaactaga gaatcaaaat ctacatgtac
     3541 tgatagattt gctattcatt tgtcaaagag cgctaggcta ttttcagcct atggaaatcc
     3601 tttattttga cccgataaaa aacttccata gaatgaaaag gaaagggaaa aaatacttta
     3661 acgagttgta tcaaattcaa gtatcttctc aatcaatttt gtttccagtt gtctacgcat
     3721 atattcatct acatacataa ccattccttt ttcttcttca taaactggtt tcatggctaa
     3781 ccgtgaaata taattttgat attttgacag aacgatggac atggcaattg aatcaccaga
     3841 agctgcggct ttgattgtcg ttagtggtat gaatggtata tatcgtttat tttgcttcat
     3901 atttgacacc caaatacttt cctagctctt ccaaagccat ttgtcgttgg taccaaacac
     3961 tgccgagact tttcccaata atatcgccaa tttcacgatc attaagaccg atgaagaagg
     4021 acataaaaat gatttcttgt ctttgactag gtaatgctgc aatcgcattt gccaactcct
     4081 cattggaaat aaagattttc ataccaagga caataaaagt accactacta ttcaaatttg
     4141 gttccaaata atagaactct gtacctaacc ctttttgttc caatatttcc attgatattt
     4201 gccgttggcg aaatctagcg tacttctttt gaatatttaa cgcctcattg cagactactt
     4261 tcttacaaaa actgtcaaac gtatgttgaa tgtgggtttc ctctgagtcg cccataatat
     4321 tctccccctc aaaaaattca ttcaactcgt tttacttcta tccctttcca ttattactac
     4381 aatctagga
//
LOCUS       DAZKLM010000142         4304 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3764
            SAMEA115706913-rid22582563.denovo.148, whole genome shotgun
            sequence.
ACCESSION   DAZKLM010000142 DAZKLM010000000
VERSION     DAZKLM010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706913
            Sequence Read Archive: ERR13196647
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4304)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4304)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287823.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287823.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,004
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,849
            CDSs (with protein)               :: 2,849
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 41 of 96
            Pseudo Genes (incomplete)         :: 52 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 15 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4304
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706913-rid22582563.denovo.148"
                     /isolate="VRE3764"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..792
                     /locus_tag="ACG842_002646"
     CDS             <1..792
                     /locus_tag="ACG842_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFQ0249125.1"
                     /translation="FCGYSKTEPRHSFGPAIREQYLIHIVLEGEGYYSINDQKYFLKK
                     GQGFVIPPGVSTFYQADEKNPWSYVWLGLSGNLVEYYLKEIGVGKNHLTFKVENLNSF
                     KGLIFETLSYDQDNLVSELSRQKQVYKFLELLLNSASYLNEGIITKKMNPFVRQTIEI
                     IRENAYQKLSVKDIAVSLSIHENYLSRLFKNDMGISIKEYISEIKISIANDLLSSTEY
                     SIQQISDKIGFSTVQSFSKAFKKANGVSPGVYRKRRLGLGIINGE"
     gene            889..3138
                     /locus_tag="ACG842_002647"
     CDS             889..3138
                     /locus_tag="ACG842_002647"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HFQ0249126.1"
                     /translation="MYKDNEMNIIYRSENQQFHLFNQEISYIFKISEDGKLLHLYYGE
                     NLPDNDYSHLIEMHHRPMTTYRNENDLLYSLEHLKQEFPEYGTTDFRHPAISLLQKNG
                     SRLSDFVYQGYEVEEGKPKLEGLPATYVDNNDESKTLKVYILDSVTNVQVELLYTIYR
                     DYPVITRSARVMNKGKDIQIIENISSLSLDLPDANYEWQQLSGAWGRERTIKSRTLQQ
                     GIQSIESTRGISSHQHNPFVTLKRKNTDENQGEAIGAALVYSGNFLIQAEVDTWDVTR
                     LQVGINPFGFNWKLNPGEIFTAPEAILVYSSKGLNAMSQTFHQLFRKRLARGKWREKD
                     RPVLINNWEATYFDFDEEKLVNIAKKAFDVGIELFVLDDGWFGERENDCAGLGDWHVN
                     PKRLPRGIGSLSEKIRSFGMKFGLWFEPEMVNKDSELYRTHPNWIISTPNRKPNHGRN
                     QYVLNFGLDEVVENIFDQMCKIIDESNLDYIKWDMNRPLTDVFDSHLSADQQGEVFHR
                     YVLGVYRLYEKLITKYPNILFESCSSGGGRFDPGMLHYAPQAWASDDSDAIERLKIQY
                     GTSMLYPLSSIGAHVSIVPNHQTNRITPIKTRGNVAFFGAFGYELDLNELSEGDLRIV
                     KEQIAFYKKYRTIIHNGTFYRLLSPFDDDNQTAWMVVSHDKKTAIVAHYKTLNEVNAP
                     YRRLKLKGLDNDGLYNVGQNSLRSGMELMRAGLVCSDASSGQINDQNRRPETFDFDSK
                     IWVITQEEK"
     gene            3141..>4304
                     /locus_tag="ACG842_002648"
     CDS             3141..>4304
                     /locus_tag="ACG842_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFQ0249127.1"
                     /translation="MNNQKSLLQKGAILSISLMLTSSQAINGVIPQMKQSLGISQSQS
                     ELLGTAPSITVILFILLSSYFAKRIGMKKTIILGLLLAGIGGIIPVFTATFQTVLISR
                     VILGAGLGVYNSLAVTYISSLYSGNTRATLLGIRNSMEALGQTILIFLAGVLVNINWT
                     ASFLVYAIAFPVALLFALKVPEIRDENSDIPENHVKEKMNPMVFALVLFAILLVMNSI
                     AISVRFASIATEIKGVNFNASNYLALMPILGILAGFMFGPINKWIGKGTLYLGIIFFI
                     ISNLLIAVSNGNMTFLLTGLFLSSITGSWCFPFIFSNLDKVTTKNTINFATSLIFIGC
                     NIGNFIAPIAMQLAQFLTRSTNLTAPFFVFAGIFIVILFVTFFATNKKNTRVTR"
BASE COUNT         1503 a          613 c          792 g         1396 t
ORIGIN      
        1 ttttgtgggt attcaaaaac agaacctaga catagttttg gaccagctat tcgagaacaa
       61 tacttaatac acatagtgct agaaggtgaa ggctattatt caattaatga tcaaaaatat
      121 tttctgaaga aaggccaagg atttgttatc ccaccaggag tatctacttt ttatcaagca
      181 gatgaaaaaa atccgtggtc atatgtatgg ttgggtttaa gtggcaatct tgtagaatac
      241 tatctaaaag aaattggtgt tggaaagaat catcttacct ttaaagtcga aaatttaaat
      301 tcatttaaag gattaatttt tgaaacttta tcgtatgatc aggacaatct agtcagtgaa
      361 ttgtccagac aaaaacaagt ttataaattt ttagaactcc tcttaaattc agctagttat
      421 ctgaacgaag ggataattac taaaaagatg aatccttttg ttcgtcaaac aattgaaata
      481 atacgtgaga atgcgtatca aaaattatct gttaaagata ttgctgtcag tctatcgata
      541 cacgaaaatt atttatcacg attgtttaag aatgatatgg gaatatcaat aaaagagtat
      601 attagtgaaa tcaaaatttc tattgcaaac gatctgttat catcaactga atattcaatc
      661 cagcaaatca gtgacaagat aggattctca acagtacaat ctttttctaa agcctttaag
      721 aaagcaaatg gtgtttcacc gggagtatat cgaaaacgta gattaggtct aggaatcatt
      781 aatggtgaat aaactaaaca tttgattttg ataaatattc gttactttaa acgcttacta
      841 tttatgtgag cgttttttat aatgcaataa agaaacggag gagagcttat gtataaggat
      901 aatgaaatga atattattta tcgatcagaa aaccaacaat ttcatttgtt taatcaggag
      961 ataagttaca tttttaagat atcggaagat ggtaaattat tgcatttata ttatggtgag
     1021 aacttaccag ataatgacta tagtcatcta attgagatgc atcatcggcc tatgacaacc
     1081 tatagaaatg aaaacgattt gctatattcg ttagagcact taaaacaaga atttcctgaa
     1141 tatggtacaa cagattttag acatcctgct atttcattac tacagaaaaa tggctctcgc
     1201 ttatcagatt ttgtctatca agggtacgaa gtggaagaag gaaaacctaa actagaaggt
     1261 ttacctgcta cctatgttga taataatgat gaaagcaaaa cattgaaagt ttatatttta
     1321 gattcagtta caaatgttca agtagaatta ttatatacaa tttatagaga ttatcctgtt
     1381 attactcgta gcgcgcgagt tatgaataaa ggaaaagata ttcaaataat cgagaatatt
     1441 agcagtttat cattggattt acccgatgca aattatgaat ggcaacaatt atctggtgct
     1501 tggggtagag aaagaactat taagtcgcgt actttacaac aaggaattca atcaatcgaa
     1561 tctactcgag gtatttcaag ccatcagcat aacccatttg ttactttaaa aagaaaaaat
     1621 actgatgaaa atcaaggaga agctattggt gcagcactag tttactcagg aaacttctta
     1681 attcaagcag aagtagatac atgggatgtt actagactac aagttggcat caatccattt
     1741 ggatttaatt ggaaactcaa tccaggagaa atatttacag caccagaagc gattctggtt
     1801 tattcaagta agggtttaaa tgccatgagt caaacttttc atcaactatt tagaaaacgc
     1861 ttagctcgtg gtaagtggcg agaaaaagac cgtcctgtct taattaataa ttgggaagca
     1921 acatattttg attttgacga agaaaagttg gtaaatattg ctaaaaaagc atttgatgtt
     1981 ggtattgaat tatttgtatt agatgatggc tggtttggag aaagagaaaa cgattgtgca
     2041 ggattaggag attggcatgt aaatcctaaa agactaccta gaggaatcgg cagcctttca
     2101 gaaaaaattc gtagttttgg tatgaaattt ggtttatggt ttgaacctga aatggtgaat
     2161 aaggatagtg agttatatcg cacacatccg aattggatta tttctacacc taatcgaaaa
     2221 cctaatcatg gtaggaacca atatgtatta aattttgggt tagatgaggt tgttgaaaat
     2281 attttcgatc aaatgtgtaa aatcattgat gaatcaaact tagattacat taagtgggat
     2341 atgaatcgtc cattgactga tgtatttgac agtcacttgt cagcagatca acaaggagaa
     2401 gtattccaca gatatgtact aggcgtatat cgcttatatg aaaaacttat aacaaaatat
     2461 cctaacatac tatttgagtc ttgttcatca ggtggtgggc gctttgatcc tggaatgctt
     2521 cactatgcac ctcaagcttg ggctagtgat gattcagatg caattgaaag gttaaaaatt
     2581 caatatggaa ctagtatgct gtatcctctt tcgtctattg gtgctcatgt atcaattgtt
     2641 ccaaatcatc aaacaaaccg tataacacct ataaaaacta gaggaaatgt agcttttttt
     2701 ggtgcatttg gatatgagct tgacttaaat gaattaagtg aaggcgattt gaggattgta
     2761 aaagaacaaa ttgcctttta taaaaaatat agaaccataa ttcataatgg tactttttat
     2821 agactattat caccattcga tgatgataat caaaccgcgt ggatggttgt tagtcatgat
     2881 aagaaaacgg ctattgttgc tcattataaa acattaaatg aggtaaatgc tccttaccgc
     2941 cgactgaaat taaaagggtt agataatgac ggactttata atgtaggaca aaatagtttg
     3001 cgatctggaa tggaattgat gagagcagga ctagtctgtt cagatgcatc tagtggacaa
     3061 ataaatgatc agaatcgacg cccagaaaca tttgattttg attccaagat ttgggttata
     3121 acacaggagg aaaagtagag atgaataacc aaaaaagctt acttcaaaaa ggagcaatct
     3181 taagtatttc actaatgtta acaagctctc aagcaattaa tggtgttatt ccacaaatga
     3241 aacagtcttt ggggatatca caaagtcaaa gtgaactttt aggaactgca ccaagtataa
     3301 ctgttatttt gtttatctta ttatctagtt actttgccaa aagaatagga atgaaaaaga
     3361 caataatttt aggtttatta ttagctggta taggaggaat tattccagta tttacagcta
     3421 ccttccaaac cgttcttatt agtcgagtaa tattaggtgc cggtttagga gtatataatt
     3481 cattggctgt gacatatatc agctctttat actctggcaa tactagagca acgctattag
     3541 gtattagaaa ttcaatggaa gctttaggtc agacaatcct tatttttctt gctggagttt
     3601 tagtgaacat taattggacg gcttcgtttt tagtctatgc aattgcgttt cctgtagctc
     3661 tcttattcgc gctaaaagtt cctgaaatta gagatgaaaa tagtgacata cctgaaaatc
     3721 atgtaaaaga aaagatgaat ccaatggttt ttgccctagt tctatttgca atcttactcg
     3781 taatgaatag tattgctatt tctgtacgat ttgcatcaat tgcaactgaa attaaagggg
     3841 taaactttaa tgctagtaac tatttagctc taatgcctat cttaggaata ctagcaggat
     3901 ttatgtttgg accaataaat aagtggatag gaaaaggaac cctatacttg ggaattatat
     3961 tctttataat ttcaaatttg ttaattgctg tgtcaaacgg taatatgacg ttcctgctaa
     4021 cgggtttatt tttatcaagt ataacaggat catggtgctt cccatttatc tttagtaact
     4081 tagataaagt cactacaaag aatacaatta attttgctac ttcattgatt tttattggct
     4141 gtaatattgg taattttata gctcctattg ctatgcaatt agcacaattt ctaacaagaa
     4201 gtacaaattt gactgctcca ttttttgtat tcgcaggaat atttattgtg atcctatttg
     4261 taacattttt tgcgacaaat aaaaaaaata ctagagtaac ccgt
//
LOCUS       DAZKLM010000143         4282 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3764
            SAMEA115706913-rid22582563.denovo.149, whole genome shotgun
            sequence.
ACCESSION   DAZKLM010000143 DAZKLM010000000
VERSION     DAZKLM010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706913
            Sequence Read Archive: ERR13196647
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4282)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4282)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287823.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287823.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,004
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,849
            CDSs (with protein)               :: 2,849
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 41 of 96
            Pseudo Genes (incomplete)         :: 52 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 15 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4282
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706913-rid22582563.denovo.149"
                     /isolate="VRE3764"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(37..1257)
                     /locus_tag="ACG842_002649"
     CDS             complement(37..1257)
                     /locus_tag="ACG842_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HFQ0249128.1"
                     /translation="MKLIGIVGSAAELSYNRKLLTFIQKQYHELFNLEIIEIKDLPLF
                     NQDDDQTNSPAIQQLNRKILMADGVIIATPEHNHTVPAGLKSALEWLSFKIHPLENKP
                     VMVVGASYYDQGTSRAQLHLRQILDAPGVNALVMPGNEFLLGKAKEAFDQEDRLIDPR
                     TVDFLGSTLASFVKFIKVVSTLAGPKKLPPEDLHATGTIATTIPDVDMSDDHWLEKAS
                     EAVGAVSGNTYVKLNRGLLTVDQINYFLDSMPMELTFADNNNQFLYYNLRQEAKEMLA
                     ARVPGQVGNPLANCHPEKSYKNVEWVIQQLRSGATDAVRVHVPKQVPEKYIVHNYQAM
                     HDENGEYAGINEYILDFKPIIEWYLQQTGQQLVGNEDAVSSASVHGGKTDAVTSASTH
                     DETAVDAVSSASVK"
     gene            complement(1277..1888)
                     /locus_tag="ACG842_002650"
     CDS             complement(1277..1888)
                     /locus_tag="ACG842_002650"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0052873 - FMN reductase (NADPH) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HFQ0249129.1"
                     /translation="MKMKFIGLVGTNSTRSTNRELLQFMKNYFVNEAEIEIVEIKELP
                     LFNKPENTELPNVVKEIAGKIEQADGVIISTPEYDHAVPASLINALNWLSYGIFPFVD
                     KPVMITGASYGTLGSSRAQAHLRQILDSPELKARIMPSSEFLLGHSLQAFDEQGRLID
                     EEQVAKLRELFKDFLLFVTITSQLQHAHQANKREAESFSWETI"
     gene            complement(1931..2863)
                     /locus_tag="ACG842_002651"
     CDS             complement(1931..2863)
                     /locus_tag="ACG842_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HFQ0249130.1"
                     /translation="MNQVIEETKIWQMMGTTITLQVGHEEPSRLLAELGEWLHVYEHR
                     FSAHDATSELMAINQAAGRQAVIVHPELFELIKLGKAHSCARNSYLNIAIGPVVQKWH
                     IGFEDALVPTKAEITELLLLTDPHQIQLDEKMKTVFLTKPGMMIDLGALAKGYIADKL
                     ITHCRERNAAFALVNLGGNIVVFGESPRRSDGAWRIGLRNPKGSREESLAVVKLNNQS
                     LVTSGIYERYLQNGSSVYHHIIDPKTGFPVKNDVASLTILSDSSVDGEIWTTRLFGAS
                     SLEVLKALEEQPGIEGAVVTKENQFLYTKGFEKI"
     gene            complement(3284..4144)
                     /locus_tag="ACG842_002652"
     CDS             complement(3284..4144)
                     /locus_tag="ACG842_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFQ0249131.1"
                     /translation="MNIEKFITARKAANLSQKEVSEGICTQATLSKFENNGQVPSLKI
                     LIKLCNRINLPLVELFSSMNVINTQLDEQLEKAEFSLITSEYEQLTQILAAIDPKVID
                     ESELIARYYYLKGFEMIFTNAPITDVLFHFDQLLMAETANTLFRLLAYTGIGMVYARE
                     KELEKAEVYFNRVFEKIYTYPIRTTEDIWRVLNIVFHCGVFFAQDGDLETSDALLNYA
                     IDICSDNHLTYYLARAAAQLAKNAQAKNKSKAEVLELIQDALAFAKINKNKKLIDELN
                     HMKKMILLIN"
BASE COUNT         1219 a          861 c          701 g         1501 t
ORIGIN      
        1 tttattcttt atttaaaact ttgcaacaga acctttttat tttacagaag cactcgaaac
       61 tgcatctact gcagtttcat cgtgtgtgct ggcgctggtt actgcatctg tttttccacc
      121 atgaacagat gcacttgaaa ccgcatcttc atttccaaca agttgttgac ccgtttgttg
      181 taaataccac tcaatgattg gtttgaaatc aagaatgtat tcattgattc cagcatactc
      241 tccattttca tcatgcattg cttggtaatt atgcacaata tatttttctg gcacttgttt
      301 tggtacatga acacgtaccg catctgttgc tccgcttcgc agttgttgaa ttacccactc
      361 tacattttta tacgattttt ctgggtgaca gttggctaat ggattcccta cctgaccggg
      421 cacacgagca gctaacatct ctttcgcttc ttggcgcaaa ttataatata aaaattggtt
      481 attattatct gcaaaagtta attccatcgg catcgaatct aaaaagtaat tgatttgatc
      541 cactgtcaat aacccacgat ttaatttgac gtaagtattt ccagaaacgg caccgactgc
      601 ttcagaagct ttttccaacc aatgatcatc tgacatatca acatcgggta ttgtcgtagc
      661 aattgttcct gtcgcatgca aatcttctgg tggaagcttt ttaggccctg ctaaggtaga
      721 cacgacttta atgaatttta caaaacttgc taatgtactg cctaaaaagt caacagttcg
      781 tggatcaatc aagcgatctt cttggtcaaa tgcttctttt gcttttccca ataaaaattc
      841 atttccgggc atcactaatg cattcacgcc tggtgcgtcc aaaatttggc gcaagtgtaa
      901 ttgcgcgcgt gaagttcctt gatcgtagta agaagcaccc acaaccatta caggtttatt
      961 ttcaagtgga tggattttaa atgatagcca ttcaagtgca cttttcaatc ctgctggaac
     1021 cgtatgattg tgttctggcg tagcaataat taccccatca gccattaaga ttttgcgatt
     1081 tagctgttga atagcaggac tatttgtttg atcatcatct tgattgaaca gaggtaaatc
     1141 tttgatttca atgatttcta aattaaatag ttcatgatat tgtttttgga taaatgttaa
     1201 taatttacgg ttatatgaaa gttcagctgc tgatccgacg atacctatta atttcatctt
     1261 tgtttgctcc cctcgattaa atcgtttccc atgaaaagct ttcggcttca cgtttatttg
     1321 cttggtgcgc atgttgcaat tgactagtga ttgtgacaaa gagtaagaaa tctttgaata
     1381 gttcacgtaa ttttgcgact tgttcttcat caatgagtcg tccttgctca tcaaaggctt
     1441 ggagtgaatg acccaataaa aattctgagc ttggcataat tcgtgccttt aactctggtg
     1501 aatccaatat ttggcgcaaa tgtgcttgtg cgcgtgaaga acctaacgtg ccataagacg
     1561 ctccggtaat catcactggt ttatccacaa acggaaaaat accatatgaa agccaattta
     1621 atgcgttaat caatgaagca ggaaccgcat ggtcatactc tggtgtgctg atgattacgc
     1681 catctgcttg ttcaattttt ccagcaattt ctttaacaac atttggtagt tctgtgtttt
     1741 caggtttgtt aaatagtggc agctccttga tttcgacaat ttcgatttct gcctcattta
     1801 caaaataatt tttcataaac tgtaataatt cacggtttgt tgaccgcgtt gaatttgttc
     1861 ctactaatcc aataaatttc atcttcattc tctttccttt ctaacatcga tttctgactg
     1921 ttcgcacgta ctaaattttt tcaaatcctt ttgtatacaa gaattgattc tcttttgtta
     1981 caactgctcc ctcgataccc ggctgttctt caagcgcttt caacacttct aaagaagatg
     2041 ctccaaataa acgtgtagtc cagatttctc catctacaga agaatcagac aaaatcgtca
     2101 ggcttgctac gtcattttta actggaaagc cagttttcgg atcaatgatg tgatgataca
     2161 ctgacgaacc attttgtaga taacgttcgt agattccaga ggtaactaaa gattggttgt
     2221 ttagtttaac caccgcaagc gactcttcac gtgaaccttt aggatttcgc aaaccaatac
     2281 gccaagcacc atccgaacgt cttggtgatt caccaaaaac aacgatattt cctcctaagt
     2341 taactaaagc aaacgctgca tttcgctctc ggcaatgtgt aattaattta tctgcaatgt
     2401 aaccttttgc taacgccccc aagtcaatca tcattcctgg tttggttaaa aaaacggttt
     2461 tcattttttc atctaattga atttggtgtg gatccgttag cagaagtaac tcagttatct
     2521 ctgcttttgt tggaaccaat gcatcttcaa atccaatgtg ccatttttgt acaaccggac
     2581 caatagcaat attcagataa ctatttcttg cacaactatg tgcttttcct aatttgatga
     2641 gctcaaaaag ttcaggatgt acaatcacag cttgtcgtcc agcagcttga ttgatggcca
     2701 ttaattcaga agttgcatca tgtgcgctga agcgatgttc ataaacgtgc aaccattcgc
     2761 ctaactctgc tagtaatcga ctaggctctt catgcccaac ttgtaatgta attgttgtcc
     2821 ccatcatttg ccagattttc gtttcttcga tcacctgatt cattttgtac cttctttact
     2881 tgaactacgg attacatgag taattagttt ttatttttgt tactgttttc acaaccagat
     2941 gatacgcctg ctagatttat ttgtcaaccg attttatata tttttttata taataaggta
     3001 attttaatat aaaattttat attgtaaaaa aacaaataag ggaaacaaac aacacatttt
     3061 tgtgtgttgc ttatttccct ctaactatcg ttttctattt gatggttaac ctacttaaaa
     3121 taaatactgc aacgacaaac ggagatcaag catgctttgt aacgtatttt acgataatta
     3181 cgactttatt ttattactct atcttgctgc catacccgtc tatccctaat catctttggt
     3241 gaattttttc aaagttagca taacaaaaat aagtattttt tccttagtta atcaatagta
     3301 tcattttttt catatgattt aattcatcaa ttaatttttt gtttttatta attttagcaa
     3361 aagctagtgc atcttgaatc aattcgagca cttctgcctt cgacttattt ttagcctgtg
     3421 cgttcttcgc taattgggca gcagctcgtg ctaagtaata agtcaaatgg ttatccgaac
     3481 aaatatcgat tgcgtaattt agtagtgcat cacttgtttc caaatcaccg tcttgcgcaa
     3541 aaaaaacacc acaatgaaag acgatattta acacccgcca gatatcttct gttgtacgaa
     3601 tcggatacgt ataaattttt tcaaacactc gattgaaata aacttctgct ttttccaact
     3661 ctttttctct agcatagacc attccaatac ctgtatatgc caataaacga aacaacgtgt
     3721 tggctgtttc tgccatcaac aactgatcaa aatggaacaa cacatccgta attggtgcat
     3781 tcgtaaaaat catttcaaat ccttttagat agtagtaacg tgcaatcagc tcagactcgt
     3841 caattacctt gggatcaatg gctgctaaaa tctgtgtcaa ttgttcatat tcactcgtaa
     3901 tcagtgaaaa ctctgctttt tctagctgtt catcaagttg tgtattgatc acgttcatcg
     3961 aagaaaaaag ttcaactaac ggaagattga ttcgattaca taatttgatt aaaattttta
     4021 acgaagggac ttgaccattg ttctcaaatt tacttaatgt tgcttgtgta caaattcctt
     4081 cagacacttc tttttgagat aagttggcag cctttcttgc cgtaataaat ttttcaatgt
     4141 tcattatttt ctctccaatc agacctcaca cgaaaatcat ttctgtctcg attttagcat
     4201 gttttctttg ggaatgcttt aggttctgtt gcaaagtttt aaataaagaa taaagtccct
     4261 tacggtatct atgatttaag ct
//
LOCUS       DAZKLM010000144         4272 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3764
            SAMEA115706913-rid22582563.denovo.150, whole genome shotgun
            sequence.
ACCESSION   DAZKLM010000144 DAZKLM010000000
VERSION     DAZKLM010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706913
            Sequence Read Archive: ERR13196647
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4272)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4272)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287823.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287823.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,004
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,849
            CDSs (with protein)               :: 2,849
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 41 of 96
            Pseudo Genes (incomplete)         :: 52 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 15 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4272
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706913-rid22582563.denovo.150"
                     /isolate="VRE3764"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            87..1388
                     /locus_tag="ACG842_002653"
     CDS             87..1388
                     /locus_tag="ACG842_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFQ0249132.1"
                     /translation="MEKLMKWVEEKLVPPMAKIGTQRHLLAIRNGVVSTLSLILIGTF
                     FMVFINLPFPGWNEFIAPYSATIVLPFRMTMGLMAIYATFVMGSDLAKSYGLDSVTGG
                     ILSLGTFFMLQVPVNVLTPEEAPLGWVLPMSSLGASGMFAGILSMIFAVEVYRFFKNR
                     NITIKMPEQVPPAVARSFEALIPGAVILTITWLIRSVAGFDVNAALLSLFEPLTNILG
                     NNLLGVLLPMFLIHLLWSFGIHGMSIVGAVVRPMWLIMLDENAKALADGTAATQLPYI
                     APEQFYQWTVTIGGAGATIVVSVLFIFFCKSKFLKEVGRFSIIPGIFNINEPIIFGAP
                     MMLNPYMFIPFNLVPLVLTIISYFAVKLEMVNRFTVLPTWTLPAPIGGYLSAGNDWRV
                     VILIMINTLIAFIIYYPFVKAYDKKMLSDEQSRGENEVQTV"
     gene            1415..1675
                     /locus_tag="ACG842_002654"
     CDS             1415..1675
                     /locus_tag="ACG842_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HFQ0249133.1"
                     /translation="MYWLILFFVFIFLLTASHLILNMLATYHIQINRWIWALASFLIV
                     ILPKIIVPHMNVLFSWGTYVLCGIFAINFMIEQHRWFVTSKL"
     gene            complement(2674..3909)
                     /locus_tag="ACG842_002655"
     CDS             complement(2674..3909)
                     /locus_tag="ACG842_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFQ0249134.1"
                     /translation="MKTILFAPAVFNLAETTRMIEVAKAMKDRYYCVFFGFSKTFAPL
                     ITESGFEYHLLAPILTKKQEEQIIKFDQLRTLKNPFTKEMVASRITSEQSLIAKFEPR
                     LIVTGSNVTIFLSARNKKIPLVYVKPYALSEPFFQSEHALMPHELRFFGPLSNPLWHW
                     TKKRIVNTRWIPNAFKHAAQKEMLPLPKTSLQMMDADLNLITTPKLFLEEEQLPENYR
                     IVGPIFAKLSTSLPEEVLTFIQRKQQERKRIIYFAMGSSGNPRMLRKILFFLQKQPDL
                     AVIAPIDYLLDDIQSDSEDLLITEYLSSHLLHEFIDFSFIHGGEGTVQTACESGKPFI
                     GVGMQFEQSCNIDYCVKYGNAIALTKPVTERKLQIALREVSSPKIRQQARTLQAVFPI
                     NGPQQAIQEIETFLSNHPI"
BASE COUNT         1237 a          683 c          881 g         1471 t
ORIGIN      
        1 tttcttaatt atataattag ttatgaacat tctttgcgtt gtcatgcaat gaatggttca
       61 tattttattg aaaaggagtg gttttagtgg aaaagctgat gaaatgggtc gaagagaaac
      121 ttgtcccacc aatggcaaaa attggtacac agcgtcattt attagcaatt cgtaatgggg
      181 ttgtttcaac attgtcattg attttgattg ggacattttt tatggtcttt atcaatttac
      241 cgtttcctgg atggaatgaa tttattgccc cttattcggc aacgattgtt ttacccttta
      301 ggatgacgat gggattgatg gctatttatg caacttttgt tatgggttcg gatctagcta
      361 aatcatatgg attagatagt gtgacaggtg gtatattatc tttaggaacg ttctttatgt
      421 tacaagttcc tgtaaacgtt ttaactcctg aagaggcacc tcttggctgg gtcttaccta
      481 tgagttcttt gggagcatct gggatgtttg cggggatctt atctatgatt tttgcagtgg
      541 aagtatatcg tttctttaaa aatcgaaata ttactatcaa aatgccagaa caagtgccac
      601 ctgctgttgc ccgtagtttt gaggcattga ttccaggtgc agtcatttta acaattactt
      661 ggctgattcg aagcgtcgcg ggctttgatg taaacgctgc cttattatcg ttattcgagc
      721 ctttaaccaa tattttaggg aataatttgc ttggtgtttt actaccaatg tttttgatcc
      781 atttgttgtg gtcatttggt atccatggca tgagtattgt aggggctgtt gttcgtccaa
      841 tgtggctgat catgttggat gagaatgcaa aggcattggc agatggtaca gctgccaccc
      901 aattacccta tatcgctcct gaacagtttt atcaatggac ggtaacaatt ggtggtgcag
      961 gagcaacgat cgtggtatct gttttgttta tattcttctg taaatcaaaa ttcttgaaag
     1021 aggttggtcg tttttctatt attcctggta tcttcaatat caatgagccc ataatttttg
     1081 gagctcctat gatgttgaat ccttacatgt tcatcccgtt caacttagtt cctttagttt
     1141 tgactattat cagttatttt gcagtcaaat tagaaatggt caatagattt actgttttgc
     1201 caacatggac attaccagct ccaatcggcg gttatctttc agcaggaaat gactggcgtg
     1261 ttgttatctt gatcatgatc aacacattga tagcatttat tatctattat ccatttgtaa
     1321 aagcttatga taaaaagatg ctgtcagatg aacaaagtcg tggagaaaac gaagtccaga
     1381 ctgtctaagt ttggttttta gaaggtggga aacaatgtat tggctgattt tattttttgt
     1441 ttttatcttt ttattaacgg ctagtcacct tatattgaat atgctagcta catatcatat
     1501 tcaaatcaat cgatggattt gggcactggc atcatttttg atcgtgattc ttccaaagat
     1561 cattgtaccg catatgaatg ttttgttttc atgggggaca tatgtacttt gtggaatttt
     1621 tgctatcaat tttatgatcg agcagcatag atggtttgtc acatcgaagc tttaaatgtt
     1681 ctaaaagtgg ttgtaacaga agtggttagc ttcaagaaat aagaagaaat ttacgaaaat
     1741 tgcttcttaa atttttgtga aatgttggct tatttttcgt ttggttgaac ttattaccgc
     1801 agatgcaagc ttttgaccac cgtttattcg gaatatggga ctgtgacaag gcttttgtca
     1861 cagccctttt ttacataaag attattattg ttttaactgt tttggcagaa agaaggagga
     1921 aaaaatggaa attttatcag caatggattt agaattagta gatctactac caaagtgatc
     1981 aagtcaaata tagatttggg agcttacaac catatagtag gggctgcagt atggtgtatg
     2041 aatggtaata tgtatgatgt gagtgtttat tcaaatcatg aaacgtgtat agaaataatt
     2101 gtgctgggaa tagcaatcag tcatggtgaa cgtgactttt agtgtatcgt tgctgacgac
     2161 atagaacatt ctgacatgat ctactcaata tgtggggatt gctggcagtt tatataggag
     2221 tatgcatcgg attgcgagat cattgtctca acagaagagg gaggttgatt atcaaagata
     2281 aagtggtaaa tcctacattt gatttagtaa ttgttgaggg gactagcggc acaaatctct
     2341 acaaagcgat gcttctagat ctatatgaca cattttaaga aaatgtattt gcctaattat
     2401 tatggtcttt cttttgaaga aacgttgaga tgatccaacc agagaaaggt gtcaaagaat
     2461 ttgactaaga ataaatggca gacgctacct atgctcattt ggatagcgtc agatctttta
     2521 ttttaaggat gattttgtgt tttttagaca ccgtaattta agtaactcta acttttcaat
     2581 ccactattta ctttttttgt attttcactg tctgggtctc gaatagcgaa attactgatg
     2641 agtctctatt aaaaaagctt tttctacttg aaactatatt gggtggttgc tcaggaaggt
     2701 ttcgatttct tgaattgctt gttgtggacc atttattgga aaaacggctt gcagcgtgcg
     2761 tgcctgctga cgaatcttgg gtgaagaaac ttcgcgcaga gcaatctgca gttttctttc
     2821 agtcactggt tttgtgaggg cgattgcgtt tccgtatttt acacaatagt caatattaca
     2881 gctttgctca aattgcatcc ccacaccgat aaacggtttg ccggattcac aggcagtttg
     2941 gacggtccct tccccgccgt ggataaaaga aaaatcaatg aattcatgca aaagatgact
     3001 agacagatat tctgttatta aaagatcttc tgagtcagat tgaatgtcat caagcagata
     3061 gtcaataggt gcaataaccg ctagatccgg ttgtttttgt aagaagaaca aaattttgcg
     3121 taacatacgc ggattgcctg aactacccat tgcgaaatag ataatacgtt ttctttcttg
     3181 ttgctttcgc tgtataaaag ttagcacttc ttcaggcaag gaagtactga gcttagcaaa
     3241 aattggacca acgattcgat aattttccgg cagctgttct tcttccaaaa atagtttagg
     3301 cgttgtgatg agatttagat cagcatccat catttgtaaa cttgtttttg gtaaaggaag
     3361 catttctttt tgtgcagcgt gtttgaaggc attggggatc catcgggtat ttactatacg
     3421 cttttttgtc cagtgccaaa ggggattgct taaagggccg aagaaacgta actcgtgggg
     3481 catcagcgca tgctctgatt gaaaaaaagg ttcactcaaa gcatagggtt taacatatac
     3541 taaaggaatc tttttattgc gggccgataa aaaaattgtc acattcgaac ctgtcacaat
     3601 cagtcgaggc tcaaacttag caatcagact ctgttcacta gtgatacgag aggcaaccat
     3661 ttctttggta aaagggtttt taagtgtacg caactggtca aattttatga tttgttcttc
     3721 ttgttttttc gtaaggatcg gcgctagtaa atgatactca aagccacttt ctgtgatcaa
     3781 aggagcaaaa gttttagaaa agccaaagaa tacacagtaa taacggtctt tcatagcttt
     3841 ggcaacctca atcattcgtg ttgtctcagc taaattgaat actgcaggag caaaaagaat
     3901 tgtcttcatg aaatcctcct tattggaatg agtctatccc ttcgtttctt actagtatac
     3961 taaaaaacac agcgttcgag tagcctatgt atattttgaa tattaaactg tctctcgctg
     4021 ttttcacaac ttctaaattt aaattttgta atcaagttga aactccaatt ttcaaaaaaa
     4081 cacgcaacaa ttttttgatc gatttatatg ttgtgccaga taaatacgag aagttgtgtc
     4141 attaaaaata aacatgagaa cataaaaaaa taaaataatt atattaattg ataggtgtgc
     4201 tgtcgtcatt atacacacgt aagaaagaaa tagttatgtt taaaaatatt cttagacttt
     4261 ctcgccatga ca
//
LOCUS       DAZKLM010000145         4266 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3764
            SAMEA115706913-rid22582563.denovo.151, whole genome shotgun
            sequence.
ACCESSION   DAZKLM010000145 DAZKLM010000000
VERSION     DAZKLM010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706913
            Sequence Read Archive: ERR13196647
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4266)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4266)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287823.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287823.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,004
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,849
            CDSs (with protein)               :: 2,849
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 41 of 96
            Pseudo Genes (incomplete)         :: 52 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 15 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4266
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706913-rid22582563.denovo.151"
                     /isolate="VRE3764"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            66..503
                     /locus_tag="ACG842_002656"
     CDS             66..503
                     /locus_tag="ACG842_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0249135.1"
                     /translation="MEWFCKFLLFLEVIHVYGFVFLTNKKAGSTMDHLYIKYIHQFSV
                     LVLGTLIIAGIVAFIIQGRDILTVHQEFFSYAFGIPLIAIRSDNKKWKKRYYILLLFL
                     CFYLPPKIFTLLGIQCLSFSFISYIIVLIFLSFIVWVFYLVKK"
     gene            598..1482
                     /locus_tag="ACG842_002657"
     CDS             598..1482
                     /locus_tag="ACG842_002657"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HFQ0249136.1"
                     /translation="MKKVLLVVNPSSGGEQAKEFEQLAIAKLESVFDEVVVLHTKKAG
                     DAKNFTREAATEGYHSVFVMGGDGTVNEGISGIAEQEHRPNFGFFPLGTVNDLARALG
                     IPLEPEEAINHFSIESVKPLDIGKINDDYFMNVVAIGSIPEAINDVDAEKKTKFGKLA
                     YFMSGIKQLASTQSYSFHVEVDGKKEEIESSILLIGLTNSVGGFETLLPNAKVDDGKL
                     HLVYLKDSSLIDTLKTLPDLLKGVDESTKNLAYLTCEQLVVSLADEEELTTNVDGDEG
                     DKLPVTIKVLPSHLQVYC"
     gene            1704..>4266
                     /locus_tag="ACG842_002658"
     CDS             1704..>4266
                     /locus_tag="ACG842_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HFQ0249137.1"
                     /translation="MNQYVKKLIASILFVFPLLMVTSVFADVEKTSESTQSENQSFDK
                     TTFSEESITSETTESQSEAPTSNTRQEENKTSETSEVAEQTSDSSEEVQVRVVSADGY
                     SEAATTEELTQLLADESVAKIRLTQPLTLDRELEIKRDIVIDFGGLAHNFGTHHIYIN
                     ETPQSIEFQNFKGTAAHPGGLIPNIDGNAIIIAYMSDFWGNRYHFTGEIKFTGTLDLY
                     DGSKLGLIYAPRATVTLDGVSGVLDVQPVKEGMNAAPGKAYFCRSYQLNVINGSQLYG
                     PYLGKFYGFLDSGDETGSGKTAPGLHILSGSKVSLDYDRADGVSDEGEAVDTLPGNVT
                     FKVSGSGSEFSVNTKINITNDNNRGIIQMRGSGSRVAVSNDGKITINTATTGGFRLQG
                     DSSQIHVSSGGQISVKMAGDGDQPGNNGMRFVGKGLSLIVEGAGSVIDIDKQSGRSSA
                     VRFENGTQKLTVTNGGSLKVRNAGDGKSYVNRLNQGNQAIQFYDASGFLESPGSADFT
                     VTGEKSNIDIRADSGATVDTTGDIDLNFMAGEKTYLVMVGKTGEDKTGIISGDVLNVD
                     LESPTYFDFQNKRIGSETNGIGGWVFQGNGDSSLKIAHSEIALWRKAGVTFDNFEKDP
                     DYFSDMTDLSINGTDLGNFVSSPSAGLTNEFAQTGAGMKNYNRISANNQLPMIESLRV
                     PTNADKKIYGHVVVPEGVEAKPRDAWTGEVEVTLRITYADTTKSPVEITALTQGQTDE
                     NEGTGYNPWGEGEQGGLFEVQVPNEERLTTGDQVQIVAAKKVRGGQEVTELDSAKTTV
                     DVIPPEPAKLSTTLINAASRSITGTGEAGALVSLKKNQAVLSETTIDDTGKFVLSI"
BASE COUNT         1396 a          714 c          961 g         1195 t
ORIGIN      
        1 gatatttttc tgtataatct taaataaaga aaacgatatt aaaaaatgca cgtagaagta
       61 gataaatgga gtggttttgc aaatttcttt tatttctaga agttattcac gtatacggat
      121 ttgtttttct cacaaataaa aaggcaggaa gtacaatgga tcatttgtat ataaaatata
      181 ttcatcaatt ttccgttttg gtattaggca cactaattat agcggggatt gttgcattta
      241 ttattcaagg tcgtgacata ttaacagtac atcaagagtt tttcagttat gcatttggta
      301 ttcctctgat cgctataagg tctgataaca aaaagtggaa gaaacgctat tacattctct
      361 tgctattttt gtgtttttat ctgccaccta agatttttac tttattaggt atacaatgcc
      421 tatcattttc ttttatttcc tatatcattg ttttaatttt cctcagtttc atagtatggg
      481 tattttatct tgtcaaaaaa taaatgaatg tttcatgaac agtacttcat cacagcctaa
      541 tttttttctt tatgctatcg ttatattaaa gaaatcaatc agaagggaaa gaaagtgatg
      601 aaaaaagttt tattagtggt aaaccccagt tcaggcggtg aacaagcaaa ggaatttgag
      661 caattagcga tagcaaaatt ggaatcggtg tttgatgaag tggtcgtctt gcatacaaaa
      721 aaagcagggg atgcaaaaaa ctttactcgc gaagcggcta cggaagggta tcatagtgtg
      781 tttgtcatgg gcggagatgg aacagtcaac gaaggaatca gtggaatcgc tgaacaagaa
      841 catcggccaa atttcggatt tttcccattg ggtacggtga atgacttggc tcgagcatta
      901 gggataccgc tagaacctga ggaagcgatc aatcattttt cgattgaatc agtcaagcca
      961 ttggatattg gaaaaataaa tgacgactat tttatgaatg tcgtcgcaat tggctctatc
     1021 cctgaagcaa tcaatgatgt agatgcggag aagaagacga aatttggaaa actagcttat
     1081 ttcatgtcag gaatcaaaca gttagcttcc acacaatcct actcgtttca cgtagaagtt
     1141 gatggaaaga aggaagaaat tgagagcagt atcttgctga taggactaac taactcagta
     1201 ggcggttttg aaacattact tccaaatgca aaagtggatg atggtaagct ccatttggtt
     1261 tatttaaaag attcttcttt gatcgatacg cttaaaacat tgccagacct attaaaagga
     1321 gtagatgaat ctacgaaaaa tctagcctat cttacttgtg agcaattagt tgtgtcatta
     1381 gctgatgagg aagaattgac gacgaatgtt gatggagatg aaggggataa attacctgtg
     1441 acaatcaaag tgttaccctc acatttacag gtttattgtt aaaatggagg ttgtgaccga
     1501 agagttgtca ggaggacatc ggttgttcgg aataagggac tgtgacaaga cttttgtcac
     1561 agcctttttt tgtacaaaaa agaaattttc acctaacaga aaaatttgcc ataaaaatac
     1621 atattttaaa tatgaaagta caattacaaa taaattaaaa cgttaaaata aaattgtcaa
     1681 aacgtcagag aggatggtgg agaatgaatc aatatgtaaa aaagctgatt gcgtcgattt
     1741 tgtttgtctt ccctctcttg atggtgacat ccgtttttgc cgatgtagag aaaacaagcg
     1801 aatcaacgca atcagagaat caaagttttg acaagactac tttttcagaa gaatcaatta
     1861 cttctgaaac tacagaatcc caaagtgagg cgcctacttc taatacaagg caagaagaaa
     1921 ataagacaag tgagacctca gaagtagctg aacaaacgtc tgatagcagt gaagaggtac
     1981 aggttagagt tgtttcagcg gatgggtaca gcgaagctgc cacaacagaa gaactgacgc
     2041 agcttctcgc agacgaatca gtagctaaga ttcgtttaac tcagcctttg actttagaca
     2101 gggaattgga gatcaagcga gatatcgtta tcgattttgg cggacttgct cataatttcg
     2161 gcacacatca catctatatc aatgagacac ctcagtcgat cgagtttcag aattttaaag
     2221 gaacggcagc tcatccaggt gggttgatac caaatatcga tggaaatgcc attatcattg
     2281 cttatatgtc cgatttttgg ggaaacagat atcattttac gggagaaata aaatttaccg
     2341 ggacacttga cttatatgat ggttctaaat taggcctgat ttacgcccct cgtgcaactg
     2401 tcacattaga tggtgtttcc ggtgtattgg atgtccaacc agtgaaagaa gggatgaatg
     2461 ccgctccggg taaagcttat ttttgtagaa gctatcagct aaatgtaatc aatggtagtc
     2521 agctttatgg accatatcta ggaaaattct atggttttct agattcaggt gatgagacgg
     2581 gcagtggaaa aacagcacca ggactccata tcttgagtgg ttcaaaggtc tctcttgact
     2641 atgatcgggc agatggcgtc agcgatgaag gagaggctgt cgatacgcta cccgggaatg
     2701 tcacatttaa agtcagtggc tcaggaagtg aattttcggt aaatacaaag atcaatatta
     2761 ccaacgacaa caatcgcgga attatccaaa tgagaggctc aggatcaaga gtcgctgtgt
     2821 cgaatgatgg gaaaatcaca atcaatacag cgactactgg cggatttcgc ctgcaaggag
     2881 attctagtca aatccatgta tcaagtggtg gacaaatctc ggtcaaaatg gctggagatg
     2941 gcgaccaacc tggaaacaat gggatgcgtt ttgtagggaa aggactttct ttgatcgtag
     3001 aaggagccgg atcagtcatc gatattgata agcaaagtgg tcgttctagc gcagttcgtt
     3061 ttgagaacgg tacgcaaaaa ctaacagtaa ctaatggagg ctcattgaag gtaagaaatg
     3121 caggggatgg caaatcttat gtgaacagac tcaatcaagg aaaccaagcg atccaatttt
     3181 atgacgcttc tggttttcta gaaagtccag ggtcagctga ttttacggtt actggagaaa
     3241 aatcaaatat cgatatacgc gcagattccg gagctaccgt agatacaact ggagatattg
     3301 atttgaactt tatggctggc gaaaaaactt atttagtcat ggtcgggaaa actggggaag
     3361 ataagactgg catcatcagt ggggatgtgt tgaatgtaga cttagaatct cctacctatt
     3421 tcgatttcca aaacaaacgg atcggaagcg agactaatgg cataggtggc tgggtatttc
     3481 aaggaaatgg cgattcttct ttgaagatcg ctcattcaga aattgcttta tggcgaaagg
     3541 caggagtaac gttcgataac tttgaaaaag atccggatta tttttctgat atgacagact
     3601 tatcgatcaa tggaaccgac ttaggtaatt ttgtatcaag ccctagtgca ggtttaacaa
     3661 atgaatttgc tcagacaggt gccggaatga aaaattataa tcgaatcagt gccaataacc
     3721 agcttccgat gattgaatcc ttaagagtac caacgaatgc agataaaaaa atctatgggc
     3781 atgtcgttgt tcccgaaggt gtcgaggcaa agccaagaga tgcttggaca ggcgaagtag
     3841 aagtgacttt acgtattact tatgcagata cgacgaaatc acctgtagaa attactgctc
     3901 tcacacaagg acaaacagat gaaaatgaag ggacaggata taatccgtgg ggagaaggcg
     3961 aacaaggcgg tttgtttgag gttcaagtac ccaacgaaga aagactaaca acgggggatc
     4021 aagtgcaaat agttgctgct aaaaaagtaa gaggcggcca agaagtgaca gaactggact
     4081 ctgccaaaac gacagtcgat gtgattcctc ctgagcctgc taaattaagt accacattga
     4141 tcaatgcagc ttctcgctca atcacaggta caggcgaagc aggagctttg gtttcattga
     4201 aaaaaaatca agctgttttg tccgaaacaa ctattgatga tacaggtaaa tttgttcttt
     4261 ctatcc
//
LOCUS       DAZKLM010000146         4040 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3764
            SAMEA115706913-rid22582563.denovo.152, whole genome shotgun
            sequence.
ACCESSION   DAZKLM010000146 DAZKLM010000000
VERSION     DAZKLM010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706913
            Sequence Read Archive: ERR13196647
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4040)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4040)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287823.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287823.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,004
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,849
            CDSs (with protein)               :: 2,849
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 41 of 96
            Pseudo Genes (incomplete)         :: 52 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 15 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4040
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706913-rid22582563.denovo.152"
                     /isolate="VRE3764"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            178..774
                     /locus_tag="ACG842_002659"
     CDS             178..774
                     /locus_tag="ACG842_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HFQ0249138.1"
                     /translation="MNIAASRKLINFISIIGLGLSIALTIYFINLGVFKDLNALRGLV
                     GDSIILGPIIFVFIQILQVVIPIIPGGISTAAGVLIFGPYAGFIYNYIGICIGSIIIF
                     LLGRRYGKPFILSMISDKTYNKYIGWLDNQNRFEKLFALAIFLPVAPDDALCLMAGLT
                     NMSVKRYTLIILIAKPLSIFLYSMALIYGGQYLSGLLG"
     gene            898..2697
                     /locus_tag="ACG842_002660"
     CDS             898..2697
                     /locus_tag="ACG842_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphoryl diester phosphodiesterase
                     membrane domain-containing protein"
                     /protein_id="HFQ0249139.1"
                     /translation="MNYLKRSLGNAWDFLKGTQAYFRDVLLMHGFILFICLPLLSSTT
                     RFILHRGAIDYLSYDNIPSIFSQHPGVLLSLIVVLMTILLLVYFEFTFLLMSVFFIKK
                     KEPISLKQLLHLTILQLKKVRPITFLFFLAYFLLILPISGLSFNSDLLSKIKIPAFIM
                     DFIFANRWIIVSSFLLVYIFLGYIGIRLIFALPEMILRDRPFRAAIRESWSLTKSRLL
                     AITGQFIVIGGTILLLSSLGYIVVILAQSMVEQFFPDYALISAVFAMTLLQGILLFNI
                     VMSTVGIFYIIVDFMDDEGFLPEIPKWFIPQAPNLRFSALKNTGLTLFAVFFGIGVCL
                     YNMDYLTSAVQTKPVTVSHRGVSTQNGAQNTLEALEKTSRDYHPDYVEMDVQETKDGH
                     FVVMHDANLRHLTGVNGTPQDLTLKELTNLTVTENGVQAPVCSFDSYLEKAHKLNQKL
                     LIEIKTSKKDSDDLVERFVHKYRKVILENHHILQSLTYQTVSDLKEAEPNFYVGYILP
                     FNIVGPPVTPADFLTMEYSTINRNFINSAHQDGKEVYVWTINDSDAMSRMMFYGVDGI
                     ITDEMGLLNESVKQTDEEITYSDKLLNFVLGVG"
     gene            complement(2975..3187)
                     /locus_tag="ACG842_002661"
     CDS             complement(2975..3187)
                     /locus_tag="ACG842_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0249140.1"
                     /translation="MVKAEVGRSYICKPIGVSHELIGCIERTYINTAVVYVESYDQRD
                     YQLINESKYRMLVKFCDISAPAELVS"
     gene            3649..3798
                     /locus_tag="ACG842_002662"
     CDS             3649..3798
                     /locus_tag="ACG842_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0249141.1"
                     /translation="MTFTKTNMQYRVAFDTTSNRFMAIDAQNENNVAFGITIEQAVRA
                     LNESR"
BASE COUNT         1189 a          689 c          711 g         1451 t
ORIGIN      
        1 ttaaaaaaaa caagttatta aataagtctt tagttggatg tttttttgag atcaatcttt
       61 tatacttatg atgaacttac aaaattgttt gttttctgtc atatcaaaaa atggcgcagt
      121 tagttgcttt tctagtaaag tatagttaac aaagaacctc taaggagttg atgaataatg
      181 aatattgctg cctctcgaaa actgataaat ttcatatcca ttatcggatt aggcctatca
      241 atcgcgctga cgatttattt tattaattta ggtgttttca aagatttgaa tgcgttaaga
      301 ggattagtag gtgattccat cattttagga ccgattattt ttgtcttcat ccaaatcctg
      361 caagtagtta ttccaatcat tcctggaggg atcagtactg cagctggtgt cctgatattt
      421 ggcccttatg ctggatttat ctataactat attggaatct gcattggttc gattatcatt
      481 tttctgctag ggcgtcgtta tggaaagcca ttcatacttt ccatgattag cgataagaca
      541 tataacaaat atattggctg gctggataat caaaaccgtt ttgagaagct gtttgctcta
      601 gctatttttc ttccagttgc tcctgacgat gcgttatgtt tgatggctgg attgacaaac
      661 atgtcagtga aacgatatac actaatcatt ttgattgcta aaccactgtc gattttctta
      721 tatagtatgg ccttgattta tggaggtcaa tatctaagtg gtcttcttgg ataatattta
      781 ttttgtaaaa ttagtcattg attgacaatg ttaaatctgg gaaagggtct gcaaaacaca
      841 ctgcaccctt tcctttttca ttagatttgc gctacaatgt gaagagcagg tgataggatg
      901 aattatttga aacgtagctt aggtaatgct tgggactttt taaaaggaac acaagcttat
      961 ttccgtgatg tcttgctgat gcatggcttt attttattta tttgtttacc cttattgagc
     1021 agtacgaccc gttttatttt acaccgaggg gcaattgact atttgtctta tgacaatatt
     1081 ccttcaattt tttctcaaca tcctggggtt ctactctcgt taatcgtagt tttgatgacg
     1141 attttactgt tagtttattt cgaatttact tttcttttga tgagtgtttt ttttattaaa
     1201 aagaaagaac caatctcttt gaagcaactc ttacacttga cgatacttca gctgaagaaa
     1261 gtgagaccca ttactttttt gtttttcctt gcttattttt tattgattct accgatcagc
     1321 ggattaagtt ttaattctga tctactgtca aaaataaaaa tccccgcttt cattatggat
     1381 tttatttttg ctaatcgttg gatcatcgtc agttcctttt tactcgttta tattttcctt
     1441 ggctatattg ggattcgact gatttttgct ttaccggaaa tgattctacg agatcgtcct
     1501 tttcgagcag ccattcggga aagctggtca ttgaccaaat cgcgtctttt ggccatcacg
     1561 ggccaattta ttgtgattgg cggtacgatt ttactccttt cttctttagg atatatcgtt
     1621 gttattttgg cacaaagtat ggttgaacaa tttttcccag actatgcatt gatcagcgct
     1681 gtgtttgcta tgacgctttt acaagggatt ttattattca atatcgtgat gtctaccgta
     1741 gggatttttt atatcattgt tgatttcatg gatgacgaag gcttcttgcc tgagatccct
     1801 aagtggttta tcccacaagc ccccaacttg cgtttctctg cgctgaaaaa cactggtttg
     1861 actctttttg cagttttttt cggtattggc gtctgtcttt acaatatgga ttatctgact
     1921 tcagctgttc agacaaaacc agtgactgtc tctcacagag gtgtgagtac ccaaaatggg
     1981 gcgcaaaata ctttagaagc acttgaaaaa acaagcagag actatcatcc tgattatgta
     2041 gagatggatg ttcaggaaac aaaagatggt catttcgtag tgatgcatga tgcaaatctt
     2101 cgtcacttaa caggggtaaa tggaacacca caggatctta cattgaaaga attgacaaat
     2161 cttacagtta ctgaaaacgg agtgcaagcc cctgtttgtt cttttgattc ttatttggaa
     2221 aaagcacata agctaaacca aaaattactt attgagatca agacctctaa aaaagattcg
     2281 gatgaccttg tcgaacgatt tgtccataaa taccgcaaag ttatcttaga gaaccatcat
     2341 attttgcaaa gtttgacgta ccaaaccgtt tcagatttaa aagaagcaga accaaacttt
     2401 tatgtgggct acatcttacc tttcaatatt gttgggcccc ctgtgacgcc tgctgatttt
     2461 ctgacaatgg aatatagtac gatcaaccgt aacttcatta attctgccca ccaagacgga
     2521 aaagaagtat atgtatggac aatcaacgat tcagatgcta tgagtcgcat gatgttttat
     2581 ggtgtggatg gtatcataac cgatgagatg ggactactaa atgaaagtgt gaaacaaaca
     2641 gatgaagaaa tcacttattc agataaactg ctaaattttg ttttgggagt cggctaatca
     2701 aaaatcgtta atgaaaaaag tctttataca acaaaaaacg cataacctgc caccgcaggt
     2761 atatgcgttt tttgtttgac gctcataatg tgtgcttaac tttctcttga ttccttcatc
     2821 gcacgaacaa tagctaaaat agcaggaatt gcagagatca ctgcaagaat aattcccatt
     2881 ttccctgaac tttttctttt tggcatatct tctcgctccc ttctgtcttt atttatatta
     2941 taagggagtt cttgcgaatt ctcaaaaata ctgtttaaga aaccaattca gcaggtgcgc
     3001 taatatcaca gaattttacg agcatgcgat acttgctttc attgataagt tgatagtctc
     3061 gttggtcata actttcaaca tacacgacag ccgtgttgat atatgtacgt tcaatacagc
     3121 cgattagttc atgagatact ccgatcggtt tgcatatata agatcttcct acttctgctt
     3181 ttaccatttc attccctctt ttctttattt ttattttgtt ttttctttat tttataataa
     3241 caaattacct tgtatttgaa aacgttttta tccatttttt tattttttat tttgagtatc
     3301 tttctgcttt ttagataact attttttaac acgatcttag acgttgtgac aaaaacgttt
     3361 gtttatattc ctatgttgtt cgtgcgtttt tttctttata ttgctaagtt atttataaag
     3421 aggtgagcat atgatttata ttcaagcaat agtatttgta accttgatga ttgggctact
     3481 tatcggatta aaagaaaata gctaaaccat ttaagaaaaa ataaataagg aaaatgataa
     3541 atgcttttta attgcatcaa aaaacgtacg atgctaaact tggtataagt tcgaaagttt
     3601 aaaggaaact ttcaagaact aaattacatt acaaaggaga tgaattttat gacgtttaca
     3661 aaaacaaata tgcaataccg cgtagctttt gatacaactt ctaaccgttt tatggcgatt
     3721 gatgcacaaa acgaaaataa cgttgcgttt ggtattacaa tcgaacaagc agtccgagca
     3781 ttaaacgaat ctagataagg agtgaaccat tccaaatggc gactgatgag atctcggaaa
     3841 atcaataaaa gacgtttgta acggtaatcc gcgacaaacg tctttttgta ttcattacat
     3901 atatttggaa attgtctctc tttatctcta gacctaaaca aaaacacaag ttctaatatc
     3961 aaccatctcg ttttttatga caattttgtt agctgtttcg atgataatat tccgttatta
     4021 tctgtataat atttaatata
//
LOCUS       DAZKLM010000147         3976 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3764
            SAMEA115706913-rid22582563.denovo.153, whole genome shotgun
            sequence.
ACCESSION   DAZKLM010000147 DAZKLM010000000
VERSION     DAZKLM010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706913
            Sequence Read Archive: ERR13196647
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3976)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3976)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287823.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287823.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,004
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,849
            CDSs (with protein)               :: 2,849
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 41 of 96
            Pseudo Genes (incomplete)         :: 52 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 15 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3976
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706913-rid22582563.denovo.153"
                     /isolate="VRE3764"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            52..2721
                     /locus_tag="ACG842_002663"
     CDS             52..2721
                     /locus_tag="ACG842_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HFQ0249142.1"
                     /translation="MKKNLKSFFRENGRYAAASFLIPFLILALVYLSIGIYPGSSRSI
                     LASDAFSQFSNFHASFRNVLLGKQSIFYTWNASLGLNYLSLISYYLGGIFTPLVFFFP
                     NQMMPDALYVLTLLKVGCAGLAFWFLARTFKIPRWGHVALSVSYALISFITAHSEIIM
                     WLDAFFYLPLVILGIHRVMDLKRPKLLFISYLLLFLSSFYMGFMIGVFSFLYFIARLL
                     TNWSEYKKTIVPYGITSLLAGGASMIIVLPAVLDLRSNGEALSQITTLKTEATSYLDL
                     IMKNMIGVYDTTKYGSIPFVYAGLLPLVLCLFYFASRKVPLKNKFLYGSLFAILIASF
                     YLVPLNLFWHGMHAPNMFLFRYAYLFSFLVLLLAGKGWEKIEQDDRGLLLGIILVVAA
                     LFAVAWGIKPEGSYTYVTMTSFVLTVVFLGIYAFAVGFYQHKKLSFNHLSILLLLIMS
                     AEALVNTNSMIHGILDDWNYASRSLYTDPYPDLKALVNETKKDSDSFYRLENLDPVSA
                     NDSINYGYSGISLFSSIRNRNSSSYLDKLGFRSRGTNLNIRYNNNTLLMDGFTGIKYN
                     ISKNESTFSKYGFRQAGQSGDYTLYQNVNALPLAFTAPLSVNDVEQPVTDNLTSQTNL
                     INTLSGLNEQYFTFYTPTLKNQLNTTITKTTTGVTYSETADNQPKELTWEVHVPANTQ
                     AYLSLFPTNFAQLESSSATITVNGTSRKTQINISGQYYDLGYYPQETTVTFTASFYGT
                     KEISFMEPKVVGLDVVAYQAAMNQIKEKGVEMEATGRKASGTVNTAEDSMLVTTIPYD
                     KGWKAKIDGKPVKVSNFKDAFLMIKVPAGKHTVTFSYLPEGFTIGAILFVLCIALFIL
                     YVWYLDRNRPFYGTIDPAIEEQPRNTHRRRRRK"
     gene            complement(3176..3922)
                     /gene="truA"
                     /locus_tag="ACG842_002664"
     CDS             complement(3176..3922)
                     /gene="truA"
                     /locus_tag="ACG842_002664"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HFQ0249143.1"
                     /translation="MVRYKAIIAYDGTNFNGFQKQPNGRTVQEEVEKTLQKMANGKEI
                     TVFGSGRTDAGVHAIGQVIHFDYPEERPLERMRFALDTQSPEDIAVRQVEIVSEDFHA
                     RYLVKEKTYQFRVDIGKPRSPFRRHYASYFPYPLDLEKIQRALPDLLGTHDFTSFCAS
                     GSSIEDKIRTIYEAKMEVNETKDELLFTFRGNGFLYKMIRILVGTLLKIGNGRLPEDS
                     IPDIIAKKDRNAAGPTAHPEGLYLYEVKYE"
BASE COUNT         1124 a          793 c          757 g         1302 t
ORIGIN      
        1 aggatatgat aaactgaata caacttcata gaaatttggt ggaaaataaa tatgaaaaaa
       61 aatttaaagt cattctttag agaaaacgga cgctacgcag cagctagttt cctgattccg
      121 tttttgattt tagcacttgt ttatctaagt atcggaatct atcctggaag cagcagaagt
      181 attttggcaa gtgatgcctt ttcacagttt tctaattttc atgccagctt ccgaaatgtt
      241 cttcttggaa agcaaagtat tttttataca tggaatgctt ctctaggatt gaattattta
      301 tctttaattt cttactatct aggcgggatc ttcacgccac tggtgttctt ttttccaaac
      361 cagatgatgc cagatgcact gtatgttctg actttgctta aggtcggctg tgccggattg
      421 gctttttggt ttcttgcacg tacgttcaag attcctcgct gggggcatgt tgcacttagc
      481 gtcagttacg cattgatctc atttatcact gcacattcgg agatcatcat gtggctggat
      541 gcattttttt atcttccatt agtgattttg gggattcatc gtgttatgga tcttaaacgg
      601 ccgaaacttt tatttatcag ttatttactc ttgtttttat ccagttttta tatgggtttt
      661 atgattggcg tattttcttt cttatatttt atcgcccgtc tattgacaaa ctggtcagaa
      721 tataaaaaaa cgattgttcc ttatggtatc acttctttac tggcaggcgg cgcttcaatg
      781 atcattgtat tgccagcagt gctggatctt cggtcaaatg gcgaggcact gagtcagatc
      841 actacgttga aaacagaagc gacttcttat ctggatctaa tcatgaaaaa tatgattggt
      901 gtttatgaca cgacaaaata cggatcgatt ccgttcgttt acgcaggtct gctaccatta
      961 gtcctctgtc tgttttattt tgccagtcgg aaagtaccgt tgaaaaataa atttttgtac
     1021 ggcagtcttt tcgccatact tatcgcaagt ttttatcttg ttccattgaa tttgttctgg
     1081 catggtatgc atgcaccgaa tatgttttta ttccgttatg cctatctgtt ctctttctta
     1141 gtgctcctgc ttgcaggaaa aggatgggaa aagatagaac aagacgatcg tgggctgttg
     1201 ttaggaatca ttttggtcgt agctgctctc tttgcggtgg catggggcat caaacctgaa
     1261 ggaagctaca cctatgtgac gatgacttcc tttgtattga cagtggtttt cttagggatt
     1321 tatgcttttg ctgtgggatt ttatcagcat aaaaaacttt cattcaatca tctgagtata
     1381 ttgttattat tgatcatgtc tgctgaagca ctggtcaata cgaacagtat gatccatggc
     1441 attcttgatg attggaatta tgcttcccgc agtttatata ctgatcctta tcctgacttg
     1501 aaagctcttg taaatgaaac aaaaaaagat tccgactcgt tttaccgctt ggaaaatctg
     1561 gatccagtat ctgcaaacga ttcgatcaac tatggttaca gtggtatcag tttgttttct
     1621 tctataagaa atcgtaattc ttcctcttac ctggataaat taggtttccg ttcaagaggg
     1681 acaaatttga atatccgtta taacaataat actttgctga tggatgggtt tacaggaatc
     1741 aaatataaca tttctaaaaa cgaaagcacg ttcagtaaat atggcttccg ccaagctggt
     1801 caaagcggcg actatacact gtatcagaat gtcaatgcat tgccactagc atttactgca
     1861 ccgttgtccg ttaatgatgt tgagcagcca gtgacagata atttgaccag tcagacaaac
     1921 ttgatcaata cattgtctgg tctaaatgaa cagtatttta cattctatac acctactttg
     1981 aagaatcagt tgaatacgac gatcacaaag acaacgactg gcgtcactta ctcagaaaca
     2041 gcagataacc agccaaaaga gctcacttgg gaagtccatg tgcctgcaaa tacacaagct
     2101 tacttaagtt tgttcccaac caattttgcg caattagaaa gttctagtgc gacgatcact
     2161 gtcaatggta catcgcgaaa aacgcaaatc aacatttctg gtcaatacta tgatcttggc
     2221 tattatcctc aagagacaac agtgacattt actgcaagtt tttatgggac gaaagaaata
     2281 agtttcatgg agcctaaagt cgttggtcta gatgttgtag cctatcaagc cgcaatgaat
     2341 cagatcaaag aaaaaggtgt cgaaatggaa gccactggca gaaaagcttc tgggactgta
     2401 aataccgctg aagattccat gttggtgaca actattcctt atgacaaagg atggaaagct
     2461 aaaattgatg ggaaaccagt caaggtcagc aatttcaaag acgcattctt gatgataaaa
     2521 gttccagcag gtaaacatac tgtcactttc agttacttgc ctgaaggatt tacgataggt
     2581 gcaatccttt ttgtcctatg tattgcattg tttattcttt atgtctggta ccttgaccgc
     2641 aatcgcccct tttatggcac gattgatcca gcaatagaag aacaaccacg aaacacacac
     2701 agaagaagac gtagaaaata aagaaacgat ggaacctgtt cagactgtcc gcaccaaggg
     2761 caatcaaaac aacgttccat cgttttttgg ttacagaaaa aataggtaac aaaaagatga
     2821 ttcacttatc aacgcctcgt ccacaagtca cgatattcat caaagatgag aagctgtttt
     2881 ttgaaattgc tctttgtgac tcagagttaa atgacaggct cacgacctct ttcttaaacg
     2941 gtgttcaatc agctgacctt cggtattagc tcaacaaacg gcaaaatatg aggagctatt
     3001 ttcccgtttg tttctctaat actcaggtca aaacgctgat ttcacgacct cttccttaaa
     3061 cggtgttcca gaagtagctt cttcgaaaat aagccgaaat tttgcaaaat tttgagaagt
     3121 aattttcgta aataccttct tatttcttga agctgaccac ttctatcata acctcttatt
     3181 catatttcac ttcatacaaa taaagtcctt cagggtgtgc tgtaggtcct gctgcattac
     3241 gatctttttt cgcaataata tctgggatac tatcttcagg caatcggcca ttgccaattt
     3301 tcaaaagcgt tcctactaag atccgtatca tcttatacaa gaagccattt cccctaaatg
     3361 taaacaacaa ttcgtctttt gtttcattta cctccatctt agcctcgtag atcgtacgta
     3421 ttttatcctc tatcgagctt cctgatgcac aaaaagaggt gaaatcgtga gtccctaata
     3481 aatcaggaag cgcccgctga attttttcca aatcaagtgg gtaaggaaaa taacttgcat
     3541 aatggcgtcg aaaaggactg cgtggtttcc ctatatccac tcgaaattga tacgtctttt
     3601 ctttgaccaa atatcttgcg tggaagtctt ctgaaacaat ttctacttgt cttacagcaa
     3661 tatcctctgg tgactgggta tctagcgcga aacgcattcg ttctaacgga cgctcctctg
     3721 gataatcaaa atggattact tgcccgatcg catgtactcc ggcatctgtc cttcccgaac
     3781 caaagaccgt gatttctttt ccatttgcca ttttttgtaa tgttttttct acttcttctt
     3841 gaacagtacg cccatttggc tgtttttgga atccattaaa atttgttccg tcgtacgcaa
     3901 tgattgcttt gtaacgtacc atgtttttct ctcctttgtt cctaaaaggt tatctggaat
     3961 aatgattttt ctataa
//
LOCUS       DAZKLM010000148         3915 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3764
            SAMEA115706913-rid22582563.denovo.154, whole genome shotgun
            sequence.
ACCESSION   DAZKLM010000148 DAZKLM010000000
VERSION     DAZKLM010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706913
            Sequence Read Archive: ERR13196647
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3915)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3915)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287823.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287823.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,004
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,849
            CDSs (with protein)               :: 2,849
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 41 of 96
            Pseudo Genes (incomplete)         :: 52 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 15 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3915
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706913-rid22582563.denovo.154"
                     /isolate="VRE3764"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(619..1176)
                     /locus_tag="ACG842_002665"
     CDS             complement(619..1176)
                     /locus_tag="ACG842_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0249144.1"
                     /translation="MRNLSNRNKILIIIVVIAVFHLGTNAVLSRIILGPKPPRPEITR
                     GEFDFRLEYEVDGERIVIEDTIVALFDGFSADAGSMAWYRTWRLHLASDRRSRNILLD
                     ELEDGRRIYYVPESANYSMGDVQKKREPNPNWYPFNGVTIEYPRNKTPEIGAKFISGL
                     EDLYDRFGIRLISWEHDPPIENRFE"
     gene            complement(1355..1966)
                     /locus_tag="ACG842_002666"
     CDS             complement(1355..1966)
                     /locus_tag="ACG842_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0249145.1"
                     /translation="MNSNRGNKMEYNGSMRIVQIIFWITSGIVIIGGVFLMLPSLIFP
                     FFALISPKIPEPEITYGEFPFRIEYELDGQINIIEDTVIAEFNGCEFSAGSMKRERRW
                     RSRLASGREDLPFGDDLGIYFSRGSAQYYMGENVQSMSLKPHIALRNLEEGFKREVDF
                     ILGESGYIRTVLNFGEAQEVLTQYGITLINWEISEPIVNNFGD"
     gene            complement(2079..2336)
                     /locus_tag="ACG842_002667"
     CDS             complement(2079..2336)
                     /locus_tag="ACG842_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0249146.1"
                     /translation="MRAVHVNPTIEITMIGHSKGGAEAANSVAINTNAIIFNPATVNL
                     SAYELDSNTYTANMVAYVVEGDALNVVFGGVYTYWRINHVR"
     gene            complement(2933..3211)
                     /locus_tag="ACG842_002668"
     CDS             complement(2933..3211)
                     /locus_tag="ACG842_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0249147.1"
                     /translation="MEKHNITGSMYVDDQWFISTDEVKIAAIRRDYLQKIHKIKDPNG
                     KKARLIITLITSNGKKLKLAGSYDNVKKFEHWYLQKDSISLNKQFEKN"
     gene            complement(3245..3496)
                     /locus_tag="ACG842_002669"
     CDS             complement(3245..3496)
                     /locus_tag="ACG842_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0249148.1"
                     /translation="MKEKPINKWSLYKNSMFGVVIFGVCAGLFLFILLQTVTLFSSNP
                     TYGWRDSIALGMFAGGYGFLLLFSILTLSPIHGLIQLWK"
BASE COUNT         1177 a          805 c          519 g         1414 t
ORIGIN      
        1 aactccttct tatttcttgg agttaaacgc ttctgtccca gcctccttat tccgaataaa
       61 tggtgtccaa aagcttgcat ctgccagatt atctcatttc ttatgacaag ctaagcaaat
      121 tatctccacc aaataattta ttcatatttt tgacttgcgc atactaacta attcttaaat
      181 tttcatacaa aatttctcaa tttcccttaa cgagtaatat ttatgtacgg tgattctcac
      241 tagtatccca atcacttttt aagttatccc gatttcaatt ctacttcaaa aatagttgct
      301 tttatgtatg ctgttagttg attagatggt catttgttga gctaggtcgt ttattttaaa
      361 caagttgttt ctttagctta tacctatctc gtctaccaaa cgcaagatgg caaacacaaa
      421 tgcatacgca tctgtatttg ccactttgat tcgtataaag ctaacaacta ttaactcctc
      481 tcagctaata gttgctctct tttttattca gattcatttt tgctgatcac agcctagtat
      541 ttcaatggtt ttgaacacta aaccaaattg ttccattaca acattcactg gctgatgaaa
      601 tggattagta gatttcgatt attcaaagcg attttctatt ggtggatcgt gttcccaact
      661 aataagacgg attccaaacc tatcgtacag atcttctaaa ccacttataa attttgcacc
      721 tatttcaggt gttttattgc gtggatattc tattgttacg ccattaaatg gataccaatt
      781 aggattaggc tctctttttt tctgtacatc tcccatagaa tagttagcac tttctgggac
      841 ataatatatt cttcgaccat cctctaattc atctaataag atatttcgtg atcgtctatc
      901 acttgctaaa tgtagacgcc acgtgcgata ccatgccatg ctacctgcat ctgcagaaaa
      961 tccatcaaat aatgcaacaa ttgtatcttc tatcacaatc ctttccccat ctacttcata
     1021 ttctaatcta aaatcaaact cccctcgggt aatttctggt cttggtggct taggacccaa
     1081 tattattcta cttaaaaccg cattcgttcc cagatgaaac acagctataa caacaataat
     1141 gattaatatt ttgtttctat ttgataaatt tctcattatt cgcacatcac ctctacattc
     1201 tcacatatta gttcgtaata ctcctttaat atcaaaatat ctactggtcg taataatatt
     1261 cgctatgaat tcctactact cgtataagaa aggccaaatc atcagctttt acttgctgat
     1321 gatttggctt gtttctattt atcgtaattg ttttctaatc accaaaattg ttaacaatcg
     1381 gttcagatat ttcccagttg attaaggtaa ttccgtattg tgtcaaaact tcttgcgctt
     1441 ctccgaaatt aagtacagtt cttatgtatc cactttctcc taatataaag tctacctctc
     1501 ttttaaaacc ttcctcgaga tttctaaggg ctatatgtgg ttttaatgac attgattgta
     1561 cattttcccc catataatat tgcgcacttc ctcgactaaa atagattcct agatcgtccc
     1621 caaaaggtag atcttctcta ccactagcta atctacttct ccatcttcgt tctcttttca
     1681 tagaacctgc gctgaactca cacccgttaa actcagctat gacagtatct tcaattatgt
     1741 taatctgtcc atctagttcg tattcaatcc taaatggaaa ttctccatag gtgatttccg
     1801 gttcgggtat tttaggtgag attaatgcaa agaaaggaaa tattaagctt ggcaacatca
     1861 aaaaaactcc tcctatgatc acaattcccg atgtaatcca aaatataatt tgtacaattc
     1921 tcattgatcc attgtattcc attttattcc ccctattact attcatatcc gcctatctat
     1981 atcgatcttc caatgcagag ataattgctt ccataccttg attttttact gagttaccta
     2041 tgcgagatat cattctccac taaggtgcat catattgtct atctaacatg attaattctc
     2101 caataggtgt atactcctcc aaaaacaaca tttaatgcat ctccctcgac aacataagct
     2161 accatgtttg ctgtatatgt gtttgagtct aattcataag cagataaatt tactgtcgct
     2221 ggattaaata taatcgcatt cgtatttata gctactgaat ttgctgcttc agcaccacct
     2281 tttgaatgtc cgatcattgt gatttcaata gtaggattta cgtggacagc cctcaccgtt
     2341 taccctgacc attatagaaa tcgcttactt ttttgaacta cgcaacaaag gatacaatgc
     2401 tcctaacttc actgtctcca tttttgtcca ctaaggaata taaaaaatag gcattaattc
     2461 atttaattcg gtcacaacac gttacctttt aaatcattat actgtatctc atacattatt
     2521 atcaataaac tattataaaa atgcaaaaaa tgctttatca atctattttg taattggtca
     2581 taatatcatt gaaatcatac cctgtatcga tttttcctat gaatctcgtc tatttgtgat
     2641 tgacgaaaaa gaggcaagac agacactgga atggtaattt tacccactat tttgctccaa
     2701 ctatttgtaa tcgtacccac ttttgaaata ccgagatgat cattttaatt gatatgctga
     2761 ttcccaagca aaaccccaag aaattaaatg ctctatcgca tctagcttct tgggttttgc
     2821 ttatataaat tccaacattt aaatttttta tgctgttcgg tctcagaaat gccgtttttg
     2881 cttatatcac aatatcaaga tgtcaaatta ttcgtgttgc cgatgacttt gattaatttt
     2941 tttcaaattg tttattaaga ctaatgctgt ctttttgcag ataccaatgt tcgaactttt
     3001 taacattatc atatgatcct gcaagtttta acttcttacc attagatgtt atcaaagtta
     3061 taattaacct tgcctttttc ccattaggat cttttatttt atgaattttc tgcaggtaat
     3121 ctctcctaat ggcagctatt tttacctcat cagtgctgat aaaccattga tcatccacat
     3181 acatactgcc tgttatgttg tgtttttcca tttcttccga gaacgaaaag tttaaggctc
     3241 gttcttactt ccaaagttga atgagtccat ggattggact tagtgtgaga attgagaaca
     3301 acaataagaa tccatatcct cccgcaaaca tccctaaggc tatactgtct ctccatccat
     3361 aagtaggatt agaagagaaa agagtcaccg tttgtaataa tatgaaaagg aataatccag
     3421 cacaaacacc aaagataaca actccaaaca ttgaattttt atacaatgac catttattta
     3481 taggcttttc tttcatttta cttcccccta ctgttctgtt cacacggaat gaattcaaag
     3541 ttcataagcc ctcctctttg aacatatgaa tacgacttgc taactatact ttaaaaaagc
     3601 ttgttttaag cggttatagg cgagttgtaa tctctattca tctcattagc aaataacttg
     3661 ccgaaaagtc tactgaaatt aaagcagata gtggaagaat tatttagcca ggatagacac
     3721 tatttgtgaa acacatgtct ttacttttga aattctatga tgaaaagcct tggaatacag
     3781 tagctcttaa ttggtaatac tttttccatt ataaataacc tcttttcatt ttatatttat
     3841 cccctctaaa aataatatct aatctaatat tttaaactta tatttccctc gctgtgctaa
     3901 tagagcatta ctaaa
//
LOCUS       DAZKLM010000149         3863 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3764
            SAMEA115706913-rid22582563.denovo.155, whole genome shotgun
            sequence.
ACCESSION   DAZKLM010000149 DAZKLM010000000
VERSION     DAZKLM010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706913
            Sequence Read Archive: ERR13196647
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3863)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3863)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287823.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287823.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,004
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,849
            CDSs (with protein)               :: 2,849
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 41 of 96
            Pseudo Genes (incomplete)         :: 52 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 15 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3863
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706913-rid22582563.denovo.155"
                     /isolate="VRE3764"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(245..466)
                     /locus_tag="ACG842_002670"
     CDS             complement(245..466)
                     /locus_tag="ACG842_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0249149.1"
                     /translation="MEEEKIFEKRWELASVEQRARYHNLMSSYRNIDWTYKEKKYLLW
                     LCQLDVNTFETFEVILDKIKNSNEKRADL"
     gene            complement(564..809)
                     /locus_tag="ACG842_002671"
     CDS             complement(564..809)
                     /locus_tag="ACG842_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0249150.1"
                     /translation="MDFPEVVKEVQEIGATISDSRSSPMNYQDAHGIKIIDQERNVRK
                     YTTINECVINENLCRAPITKHIKNRSKAKDGRTFTTF"
     gene            1049..1663
                     /locus_tag="ACG842_002672"
                     /pseudo
     CDS             1049..1663
                     /locus_tag="ACG842_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337511.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
     gene            complement(2033..3034)
                     /locus_tag="ACG842_002673"
     CDS             complement(2033..3034)
                     /locus_tag="ACG842_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4062 domain-containing protein"
                     /protein_id="HFQ0249151.1"
                     /translation="MKTINLIKVFIASPGDIAAKRNEVEETIYRWNIENTDNTKVILM
                     PIRWETNAIPTYINNSSGQSVINKQIVENSDILLAIFGHKLGSPVDNYRSGTIAEIDY
                     FYKNSKGEVGVFFLDEPVPQVLAEEYIEVKKYRDSLEDKGFYGTYSKDNIRRFLTKKV
                     NYLIEKAQNNLESKRFDLDSISYKTPSVINKKVTGTDKDFDDNLDIFDPIEFDNDERL
                     LLIYAFMDENSIFSTDNSTYRKVVQWESDNNLRSYLSDRVFDVLEKLSKSGILKEEYD
                     SSFDEYPSRYILISEDYKKFKRYFLKNEKKISVIMEKYKAPIQADPFSNLDESDLPF"
     gene            complement(3193..3459)
                     /locus_tag="ACG842_002674"
     CDS             complement(3193..3459)
                     /locus_tag="ACG842_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFQ0249152.1"
                     /translation="MMIKKLIEEKQKDKDFAEAYDREGRKLETAVALYHAREEAGLTQ
                     AELAQKAHTTQSTIARIERGDNVSFDKLDDIARALGKKLVVEFA"
     gene            complement(3449..3805)
                     /locus_tag="ACG842_002675"
     CDS             complement(3449..3805)
                     /locus_tag="ACG842_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFQ0249153.1"
                     /translation="MEKISFETFKRPNGHDEFLEWLETLPKKDSAKLLRTIEETEKNG
                     MLVAQRLKWVKKLDTNLYELRSKVGSNIQRAIYFHVVNGRYVITHGFTKKTEKTPISE
                     LKHAKELREEWFTNDD"
BASE COUNT         1222 a          698 c          512 g         1431 t
ORIGIN      
        1 ttactatata cttcttccca gaacttgtcc gtctccttca aattatctct acctctcgag
       61 ttcattgggt ggtttgccca tttattaaac atggaatcac ctttctcata ctcatatatt
      121 tatgtcctat ttcaattata tttatatatg cttaaataaa tttgtatcct gaaatttatt
      181 gtaatatgaa gctgtatttt acatatagta accaaagagt taataaaata aaaagagcga
      241 accattatag atcagctctt ttttcattgc tgtttttaat cttatctaga atcacttcaa
      301 atgtttcaaa ggtatttaca tctaactgac ataaccaaag caagtacttc ttttctttgt
      361 aagtccaatc aatattacgg taagaggaca ttagattatg gtaccttgct ctttgttcaa
      421 ctgaagccaa ttcccagcgt ttttcaaata ttttttcttc ttccattatt gatccctcgc
      481 aagacgctac ctcaaactta aataatgggc agcgaaagtc taattcctta gatctaagta
      541 tagcaaacaa atatgaaaaa aacttagaag gtagtaaaag ttcttccgtc ttttgctttg
      601 cttctattct taatgtgttt cgttatcggt gccctacaaa gattttcatt aattacacac
      661 tcatttattg ttgtgtattt tctgacattt ctctcttgat caattatttt aattccatgg
      721 gcatcctgat agttcatagg agaggagcgg ctatcagaaa tagttgcacc tatctcttga
      781 acttctttta caacttctgg aaaatccatc catcctgctt cagctgtcaa aagtaataat
      841 ctataatatt tatctttcct tttcaatcta agaaatcgtc accccattct ttttcttcta
      901 ttataaacta tttaactttt agaattcaaa aaccgtttgt ctatttagag tttaccctaa
      961 atagacaaaa atataacatt gaaaaattta gagttctaac gtatgattag ggtgtattat
     1021 aaacgttata gaaaagtagg gaataaattt gaaaattgct tatattagag tttcatccat
     1081 cgatcaaaat gaacaacgtc aaattgaaga aatgaaaaag tttggcgctg aaaaaatttt
     1141 tatcgaaaaa caatctggag cgacgattag tcatcggccg attttccagg aagctttaga
     1201 ttttgtcaga gagcaagata tttttattgt agaagccatt gatcgacttg gacgcaatta
     1261 tgacgagatt atcgattcag tgaattatct aaagaagaag aatgtgcgac ttattatcac
     1321 cagtttaccg atcatggctg aagcaattgg aaatcctctc ctagataaat tcatcaaaga
     1381 cttaatcatt taaatctcag ccatgattgc tgaacaagaa cgtactgaat cgaaacgacg
     1441 ccaggcacaa ggaattaaaa tcgctaaagc aaacggcgtc tataaaggtc gtcctaagtt
     1501 atacagtgca gacaccaaag atcctcaacg tcgccttgtc tataggagta tcgttcagga
     1561 tcttgagaat ggggttgcta tctcaaagat tgcaacagac tacaatgtga ctaggcaaac
     1621 aatttaccga ataaaaaaag aaatcgacca actaattgta tgatttcttt tttgaatcac
     1681 gtaattatta attagctttg tcatcaatac agacaatcat tttagattca tctagctaag
     1741 ttgattacat ttatgttaaa aagaatttag ttaacataaa tataattgat gcataaatcg
     1801 tccttattta aaggcattta acattgacaa cttagtataa tgtatcttat gtaaactaat
     1861 aagatttgaa ttttatttta tacacactcg ataatagatc aattttatgg tcccccgcat
     1921 ttacgattgc acaagagaaa cataaagtcg acctattagc atttcttttc aattcaaaca
     1981 ttaaaaaagt ttatacctct gctactaaaa aatgaaatta tctatttaaa atttaaaacg
     2041 gtaaatctga ttcatccaaa ttggaaaatg gatcagcctg tattggagct ttgtatttct
     2101 ccataataac agatatcttt ttttcatttt ttaaaaaata ccttttaaat ttcttatagt
     2161 cctctgaaat caaaatatag cgtgatggat actcatcaaa agaagaatcg tattcttcct
     2221 ttaaaattcc cgatttactt aatttttcta gtacatcaaa tactctatca ctcaagtaac
     2281 tccttaaatt gttatcactt tcccattgaa ctacttttct ataagttgaa ttatcagttg
     2341 aaaagataga attttcatcc ataaacgcat atattagcag caatctttca tcattgtcaa
     2401 attctattgg atcaaaaata tctaagttgt catcaaaatc tttatcagtt ccggtaactt
     2461 ttttgttaat tactgaaggt gtcttgtaag aaatagaatc taaatcaaag cgttttgatt
     2521 ctaaattatt ttgtgctttc tcaattaaat aattcacttt ttttgtgaga aaccttctga
     2581 tattgtcttt gctatatgtt ccataaaaac ccttatcttc taaagagtcc ctgtacttct
     2641 taacttctat atactcttca gccaaaactt gtggaactgg ttcatctaag aaaaaaaccc
     2701 ctacctcgcc tttgctattt ttataaaagt aatcaatttc tgctatagtt cccgatctat
     2761 aattatcaac tggagaaccc aacttatggc caaaaatcgc taataatata tcactatttt
     2821 ctacaatttg tttattaata acactttgcc cagagctatt atttatataa gtaggaatcg
     2881 cattcgtttc ccatcgaatt ggcatcaaaa ttacttttgt attatctgta ttttctatat
     2941 tccatctata tatggtttct tctacttcat tcctctttgc tgctatatca cctggtgatg
     3001 ctataaatac ttttattaga tttatggttt tcatttattg tacctttctc tttatgtctc
     3061 tttaaagtaa tttttgtata aaatttattg acttttacac tataggacaa cttttatcta
     3121 tttagtacaa aaaggcccac taaactaatt gattagttag gagcctatta caggtatatt
     3181 ttcaactcgc ttttaagcaa attctactac cagctttttg cctaatgccc tagcgatatc
     3241 atcaagttta tcgaaagata cgttatcccc tctctcaatt cgagcaatag tagattgagt
     3301 tgtatgtgct ttttgtgcaa gctccgcttg agttaggcct gcttcctccc gtgcatgata
     3361 aagagcaacc gcagtttcga gttttcttcc ttcacggtca tatgcttctg caaaatcttt
     3421 gtctttttgc ttttcttcaa tcaatttttt aatcatcatt tgtgaaccac tcctctctaa
     3481 gttcttttgc atgtttaagt tcacttattg gtgttttctc cgttttttta gtaaatccat
     3541 gggtaatgac ataccttcca ttgaccacat ggaaatagat agctctttgg atgtttgatc
     3601 ctaccttact tcttaattcg tacaaattag tgtctaattt tttcacccat ttcaaccgtt
     3661 gagctactag catcccattc ttttctgttt cttctattgt acgtaaaagc ttcgcactat
     3721 ctttttttgg taatgtttcc aaccattcta aaaattcatc atgaccatta ggtcttttaa
     3781 atgtttcaaa agatattttt tccataccca attatatcat atattgattt tatattatag
     3841 tatataacat aaaaattcga att
//
LOCUS       DAZKLM010000150         3698 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3764
            SAMEA115706913-rid22582563.denovo.156, whole genome shotgun
            sequence.
ACCESSION   DAZKLM010000150 DAZKLM010000000
VERSION     DAZKLM010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706913
            Sequence Read Archive: ERR13196647
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3698)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3698)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287823.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287823.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,004
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,849
            CDSs (with protein)               :: 2,849
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 41 of 96
            Pseudo Genes (incomplete)         :: 52 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 15 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3698
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706913-rid22582563.denovo.156"
                     /isolate="VRE3764"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1049
                     /locus_tag="ACG842_002676"
     CDS             <1..1049
                     /locus_tag="ACG842_002676"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD/NAD(P)-binding oxidoreductase"
                     /protein_id="HFQ0249154.1"
                     /translation="FSQEYDKVILATGSKQIMLDIPGNNLANIFSIKNFPNAKEIFKA
                     LDDSTIKKVGIIGAGYIGVEIAEAIRKRGKEVYLFDVADRVLSTYYDRSFSDKVEEIL
                     SKNGIHLCLSESPKKYLGRKKIEKIVTTSGTQYSMDMVVQAIGFLSNSPIGEKELLRD
                     VSGAYLTNEYQQTNIKDIYAIGDCATTFFTSINRSSVDFSISNALRTAYIATQHINNQ
                     DFTPSGSQFTNGFSIFNYNFYAAGLNLKTARLLSESIDYIEIEDFIRPAFLRKNSRVK
                     LRISFEKNTKRIVGVQLCSQEDLSGLLSMFSLAIEEQITLDKLKMLDYLFHPSFSQPY
                     NFILKAVTGLNIET"
     gene            1161..2564
                     /locus_tag="ACG842_002677"
     CDS             1161..2564
                     /locus_tag="ACG842_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HFQ0249155.1"
                     /translation="MKKSLKLASAVILMSQLLIATPFHVLASENSTYKIAKERTETTE
                     MSNSDVTAESSELLTTSSSESSTTSNAESSTTSSSESSTTSNAESSTTSSSESSTISS
                     TESSTTSSSTSEITSSKVTKPNHIESSKKEAPKTEGQTKTFSTPQQIESTESFQSQPI
                     LSGNEEIHFDKNESVENFIMKIGESARKIGQKYDLYASVMIAQAILESASGQSQLAQA
                     PNYNLFGIKGTYNGNFVIMVTNEDLGNGTLYTTQSKFRVYENYEESFEDYAKLLTKGI
                     SGNKDFYAGALKANSKTYREATKFLTGRYATDTQYYLKLNELIKTYDLTNYDKEVKGP
                     QMSKTGYQVPIHNYSITSLFGNRGNEFHRGLDMAATQGEPIHAVKAGKVVKAEFHYSW
                     GNYVVIQHEDGSTALYAHQQQYLVKTGDNVLQGQIIGYVGSTGNSTGPHLHLEICKDS
                     SLSQSMLVDPKTLLFGD"
     gene            2913..3545
                     /locus_tag="ACG842_002678"
     CDS             2913..3545
                     /locus_tag="ACG842_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AP2 domain-containing protein"
                     /protein_id="HFQ0249156.1"
                     /translation="MKERYGHLTVVEEITHNGYKKYRCLCDCGNETIVYRSNLLSGRT
                     KSCGCGNYKNRHKYKNLTNKTFGKLVAIKPTKLRSEGTVVWECQCLCGRKKLVSAKRL
                     LKGDVKSCGCLKSKKHHIENKKFGLLTAKYPLDNNCSWKTKWVCHCECGSMCEISYSN
                     LFFGHTQSCGCLKRQETRTCVEGTVIECLTSKLPKNNTSGVKGVYRSKEK"
BASE COUNT         1398 a          506 c          690 g         1104 t
ORIGIN      
        1 gattctctca agaatatgac aaagttatat tagctacagg ttcaaagcaa attatgctag
       61 atataccggg gaataattta gctaatattt tttcgataaa gaattttcca aatgcaaaag
      121 agatttttaa agctttggat gatagtacta ttaaaaaagt aggaattatt ggagcaggat
      181 atattggagt tgaaattgct gaagccataa gaaaaagagg gaaagaagta tatcttttcg
      241 atgttgcaga tagagtgttg tcgacttatt acgaccgtag tttttcagat aaagttgaag
      301 aaatactatc aaaaaatgga atacatcttt gtctatcaga aagtcctaaa aaatatttag
      361 gaagaaaaaa gattgaaaaa atagtcacta caagtggtac ccaatactct atggatatgg
      421 tcgtacaagc tataggattt ctatctaata gtccaattgg ggaaaaggaa ctcttgagag
      481 atgttagtgg agcatatcta actaatgaat accagcaaac aaatataaaa gatatatatg
      541 ctattgggga ttgtgctaca acttttttta cgtccattaa tagaagtagt gttgattttt
      601 ctatttctaa tgctctaaga acagcttata ttgcaactca acatattaac aatcaagatt
      661 ttacaccttc tggttcccaa tttacaaatg gcttttctat atttaattac aatttttacg
      721 cagcaggttt gaatttaaaa acagcacgat tattaagtga gtctatagat tatatagaaa
      781 tagaagattt cattagacct gcttttttga gaaaaaatag tcgtgtaaaa ttaagaatta
      841 gctttgaaaa aaatacaaag agaatagtag gtgtacaact ttgtagtcaa gaagatttat
      901 ctggactatt atcaatgttt tctcttgcta tagaagaaca aattacatta gacaaattga
      961 aaatgttaga ttatttattt catcctagtt ttagccaacc ctataatttt attttaaagg
     1021 cagtaacagg attaaacatt gaaacataat cgttactaaa tgaaatcaag aaatatactg
     1081 tattactcaa ttcattgaaa atatttatca taatttttaa tagaaaacac tattaaagag
     1141 aaaaaagaga ggtaaatcaa atgaaaaaat ctttaaaact tgcatcagca gttatattaa
     1201 tgtcccaact ccttatagca acacctttcc atgtgttggc aagcgaaaat tcaacataca
     1261 aaattgcaaa agaaagaacc gagacgactg aaatgagtaa ttccgatgta acagcagaga
     1321 gtagtgaact tttgacaact tcatcaagtg aaagtagtac gacttcaaat gccgagtctt
     1381 caacaacttc atcaagtgaa agtagtacga cttcaaatgc cgagtcttca acaacttcgt
     1441 caagtgaaag tagtacgatt tcaagtaccg aatcttcaac aacttcaagt agtacaagtg
     1501 aaataacttc ttcgaaagtg acaaaaccaa accatataga atcttcaaaa aaggaagcgc
     1561 caaaaacgga aggacaaaca aaaacttttt cgacaccaca gcaaatagaa tcaacagaat
     1621 ctttccaatc acaaccaata ctatctggta atgaagaaat tcattttgac aaaaatgaat
     1681 ctgtagaaaa ttttattatg aaaattggtg agtctgcgag aaaaattggt cagaaatatg
     1741 atttatatgc ttctgttatg attgcacaag cgatcttaga atctgccagt ggtcaaagcc
     1801 agttagctca ggcaccgaat tacaatttat ttgggataaa aggaacttat aatggaaact
     1861 ttgtcattat ggtaacaaac gaagatttgg gtaatggaac gctttatacg acccaatcaa
     1921 aatttagagt gtatgaaaac tatgaggaaa gctttgaaga ttatgccaaa ttattaacta
     1981 agggaattag tggaaataaa gatttttatg caggagcatt aaaagctaat agtaaaacat
     2041 atcgagaagc aactaaattc cttacaggac ggtatgcaac agatacacaa tactatttga
     2101 agctaaatga attaattaaa acctacgatt taaccaacta tgataaagaa gttaaaggtc
     2161 cacaaatgag taagactgga taccaagtgc ctattcataa ttattcaata accagtttgt
     2221 ttggaaatcg tggaaatgaa tttcaccgag gacttgatat ggcagctaca cagggtgagc
     2281 cgattcatgc agttaaagct ggtaaggttg ttaaagcaga gtttcactac tcttggggaa
     2341 attacgtggt gatacaacac gaagatggat caaccgcgtt atatgctcac cagcaacaat
     2401 acctagtaaa aactggagat aatgttttgc aaggacaaat aattggctat gtaggaagta
     2461 ctgggaatag tactggccca caccttcatt tggaaatttg caaagatagt agtctttctc
     2521 agagcatgtt agttgatccg aaaaccttat tattcggaga ctaaaaaagg taatcaatct
     2581 gacaaagtaa caagaaaatg gttggattct tagacctttg atcaggatat tgagactgga
     2641 attgatatct cgattacgat gggtttgaca aataaaacaa gtccattatc gaatatcgag
     2701 tgtattattg ccgtctttat gctcatattt tgagcaggtt tcactagatg aaaacaattt
     2761 atctatcttg aggatttcgt gtacgtatcg ttcagtcttg tattgtaaat tagtcataaa
     2821 atcagactaa gaaaaattgg aaatattttt ttctgattta ttaaaagtta attgaaatta
     2881 tatggtactt ctttcttggg agggtaggtg cagtgaagga aagatatggt caccttactg
     2941 ttgttgagga gattactcat aatggatata aaaaatatcg ttgcctatgt gactgtggga
     3001 atgaaacgat tgtatatcga tcaaacttac tatcaggtag aaccaaaagt tgtggatgtg
     3061 gaaattataa aaataggcat aaatataaaa atttgacaaa taaaactttt ggtaagttag
     3121 ttgcaatcaa gccaactaaa ctgcgaagtg aaggaactgt tgtttgggaa tgtcaatgtc
     3181 tgtgtgggag aaaaaagtta gtatcggcaa agcgacttct aaaaggtgat gtaaaaagct
     3241 gtggatgttt gaaatcaaaa aagcatcaca ttgaaaataa aaaattcgga ttacttactg
     3301 caaagtatcc tttggacaat aactgttcat ggaaaacaaa atgggtttgt cattgtgagt
     3361 gtggcagtat gtgtgagata agttatagta acttattttt tggacatact caaagttgtg
     3421 gatgtttaaa aaggcaagaa acaaggacat gtgttgaagg gacagtaata gagtgtctaa
     3481 cgtcaaaatt accgaaaaat aatacaagtg gtgttaaggg agtttatcgt tctaaagaga
     3541 aatagattgc gtatataact ttaaaaaaaa agaggtacta tttaggatcg tatgattcta
     3601 ttaagagtgc agctcaagct agaaaagatg ctgaaaataa attgtttcaa cctttgattg
     3661 acaaatatcg aggtaatcat taattattgt ggataata
//
LOCUS       DAZKLM010000151         3665 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3764
            SAMEA115706913-rid22582563.denovo.157, whole genome shotgun
            sequence.
ACCESSION   DAZKLM010000151 DAZKLM010000000
VERSION     DAZKLM010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706913
            Sequence Read Archive: ERR13196647
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3665)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3665)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287823.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287823.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,004
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,849
            CDSs (with protein)               :: 2,849
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 41 of 96
            Pseudo Genes (incomplete)         :: 52 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 15 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3665
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706913-rid22582563.denovo.157"
                     /isolate="VRE3764"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..278
                     /locus_tag="ACG842_002679"
     CDS             <1..278
                     /locus_tag="ACG842_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="site-specific recombinase"
                     /protein_id="HFQ0249157.1"
                     /translation="ELIKLANKGQDYEHLADEIDELRDKRQLLLVEDASLSGENERIN
                     ELIEFIRKNKFRTLEYDDKLVRKIIQSVTVYEEHFVISFKSGIEMEI"
     gene            421..642
                     /locus_tag="ACG842_002680"
     CDS             421..642
                     /locus_tag="ACG842_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0249158.1"
                     /translation="MTTAEMIKELCEQMNISVSELARRIGQTPQNFNKKLQRETVTLD
                     ELKAIADVLGVKFVQAFILPDGNEIKIGN"
     gene            658..1500
                     /locus_tag="ACG842_002681"
     CDS             658..1500
                     /locus_tag="ACG842_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0249159.1"
                     /translation="MKIITINDVEYAVFAANEGTSKPQPHIIETKSGTIPEGKQLSLL
                     KEYLKQNDISPIKGATTYWCIDKVLKLDSSKEKTISETIHKQKYLSLTEENIEKQHKF
                     VGASSNYGKEGLIIHDVLNAFPLHNDLNTIAMKIAVIDVTNSTHLSQYKSRLSLYDLA
                     KVILEIPNFDDRLAKGDPQLINIIARNIGAVNMFSFASKYCTYHNVEVCGRDDYSIFD
                     GIVKNTLPHYIQGLTTNKIDTWRRSFDYEAFNECVGKLLDENNIHIPFRRRKLDHFLW
                     YANR"
     gene            1563..3365
                     /locus_tag="ACG842_002682"
     CDS             1563..3365
                     /locus_tag="ACG842_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HFQ0249160.1"
                     /translation="MGLFDFLKKKQGKVSKHVDYQLASSLFRTGKVFAEKLEVDISNV
                     DEFRKTFIAFDVETTGLNSSSDRIVEIGAVLFKEGVPIQSFGTLVNPKVRIAASASAI
                     NNITNEMLNSAPNEQEVYPQLVEFLGDALDGKITMCAHNASFDFGFLSNTFSRLGYDA
                     KIKYVDTLSLSRKHIKGIVNHKQETVAEYFGLINDTAHRAESDADICGKIFWGVLDII
                     EESLEEQKKQIEKMTPDIKELEACAFIQDIIEKKGCDTSWIRYRKNSNNYVDVTCLYT
                     FLKFKFAKKGNYIIIRKDATNGIDLPIEPCTVSEGGTTNVRVYFRSPFDLTPLSNYIF
                     SAYSDCYNSMNEYMSVSNYTKKEAEQSLSMLKSISKSAMKELLFDAKNRGYDETEVNL
                     NIEKVISREDVIINGLHNRVSLNKIRNIGNWEKGFDAGYPYWERGETARKNGKVDEAI
                     ALFDKARYNGYDAPALYDSYAKAYRKIKDYDNEILILDEGIMRKTRHDVGTLEARRDK
                     AIKLLFAKQEAERIAKEKSDFLKANKKEDAISEPSKKRGRQIIQMTDDGTIIKEFDTI
                     ASAVKEVGVNSKSIRDAANGVQKHAGGYYWKYKD"
     gene            complement(3424..>3665)
                     /locus_tag="ACG842_002683"
     CDS             complement(3424..>3665)
                     /locus_tag="ACG842_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HFQ0249161.1"
                     /translation="PPRKNMDKSEKLDHSLLGKQRKTVGTVFSSLEKLGCQNFNSRSV
                     KGLESRFESILLAYSVLLSRAQRRFEGTLRYSLGY"
BASE COUNT         1308 a          519 c          746 g         1092 t
ORIGIN      
        1 aagaactcat caagctggct aataagggtc aagattatga acatctagca gatgagattg
       61 atgagctgag agacaagcga cagctccttt tagtagaaga tgcctccctc agtggcgaga
      121 acgagcgaat caatgagctg attgaattta tccgcaagaa caaattccgt accttagagt
      181 acgatgataa gcttgtaagg aagataatcc agagcgttac agtctatgaa gaacacttcg
      241 tcatatcctt caaatctggc atcgaaatgg aaatatgaaa accagaagta aatagcccat
      301 gattctacag tagagtcgtg ggttttcttt tgcttgctga ttataataat attttaataa
      361 ctattgttat tataaaccaa atgatttata atattcttag cgtaaacttg gaggtgctgt
      421 atgactacgg cagaaatgat taaagaactg tgtgagcaaa tgaatataag tgtttccgaa
      481 cttgctagac gtattggaca gactccacag aatttcaata agaaattaca acgagaaacg
      541 gtaaccttag atgagttgaa agcgattgct gatgtgctag gtgtcaagtt tgtgcaggct
      601 tttatattgc ctgatggcaa tgaaataaag ataggtaatt aggaggtgta atttgtattg
      661 aaaatcataa caataaatga tgttgagtat gctgtatttg ctgctaatga aggtacttcc
      721 aaaccacaac ctcatatcat agaaacgaaa tctggtacta ttccagaagg aaaacaactg
      781 agtttattaa aagaatatct taagcagaat gatatttccc caataaaagg agcaacaaca
      841 tattggtgca ttgacaaggt tcttaagctt gattcaagta aagaaaaaac tataagtgaa
      901 acaatccata aacaaaaata cttatcttta actgaagaaa atattgagaa acagcataag
      961 tttgtaggtg caagctctaa ttatgggaaa gaaggtctaa taatacatga tgttttgaat
     1021 gcatttcctc ttcataatga tttaaatact attgcgatga agattgcggt aattgatgta
     1081 acaaattcaa cacatctttc tcaatataag agcaggttgt ctctatatga tttggctaaa
     1141 gtaattctgg agattccaaa ttttgatgat cggttggcaa aaggagatcc tcagttgata
     1201 aatattatcg caaggaacat tggtgcagtt aatatgttct catttgcatc caagtactgt
     1261 acatatcata atgtggaagt gtgtgggaga gatgattact ccatctttga tggtatcgtt
     1321 aaaaatactc ttccacacta tatccaagga ctaactacga ataagattga tacgtggcga
     1381 agaagttttg attacgaggc atttaacgaa tgtgttggaa aactacttga tgaaaataat
     1441 atacatattc cttttagaag aagaaagtta gatcacttct tgtggtatgc gaataggtag
     1501 aatataagaa aaaggcatcg taaaagtgat tctggataaa attaatgggg gaggaaataa
     1561 tagtgggatt atttgatttt ctaaagaaaa aacaaggaaa ggttagtaaa cacgttgatt
     1621 atcaactggc ttctagtcta tttcgtactg gtaaagtttt tgctgaaaaa ttggaagtgg
     1681 acatatcaaa tgtagatgag tttcgtaaaa cttttatcgc ttttgatgtg gaaacaactg
     1741 gcctaaattc ttcttcagat agaattgtag agattggtgc ggtgctattt aaagaaggag
     1801 ttccaattca atcattcgga actcttgtca atccaaaagt tagaattgca gcctcagctt
     1861 cggcaataaa taatataact aatgaaatgc taaactctgc tccaaatgag caggaagttt
     1921 acccccagtt ggtagaattt ttaggagatg cactagatgg aaaaatcact atgtgtgcac
     1981 acaatgcaag tttcgatttt ggatttttaa gtaacacatt ttctagactt gggtatgatg
     2041 ctaaaattaa gtatgttgat actctgagtc tctctagaaa acatataaaa ggcattgtaa
     2101 atcataagca agagactgta gcggaatatt tcggattaat aaatgacact gcccatagag
     2161 cggaatcaga tgcagatata tgcggaaaga ttttttgggg tgttttagat attattgagg
     2221 agtcgttgga agaacaaaaa aagcaaatag aaaagatgac tccagacata aaagaacttg
     2281 aagcttgtgc ttttattcaa gatattattg agaaaaaagg atgcgacacc agttggatca
     2341 ggtatcgaaa aaacagtaat aattatgtcg atgtaacttg tctatataca ttcttaaagt
     2401 ttaagtttgc taagaaggga aattacatca ttattagaaa agatgctaca aatggaattg
     2461 atttacctat agaaccttgt actgtaagtg aaggcggtac aacaaatgtg cgagtctatt
     2521 ttcgtagccc ctttgactta acgcctcttt caaattatat tttcagtgct tatagcgatt
     2581 gttataattc tatgaatgaa tatatgagtg ttagtaatta cactaaaaaa gaagcagagc
     2641 aaagccttag tatgctgaaa tctatttcaa aatcagcgat gaaagagtta ttattcgatg
     2701 caaaaaatcg tggatatgat gagactgaag ttaacctaaa tattgaaaaa gtgatatcta
     2761 gagaagatgt aattattaac gggttgcata atcgagtttc attaaacaag atacgaaata
     2821 tagggaattg ggagaaagga tttgatgcag gttatccata ttgggaacgt ggagaaactg
     2881 ctaggaaaaa tggcaaagta gatgaagcta ttgcactttt tgataaagct cgatataacg
     2941 gctatgatgc tcctgcgcta tatgattctt atgctaaagc ttatcgaaaa attaaggatt
     3001 acgataatga aattctcatt cttgatgaag gaattatgcg taaaacacgg cacgatgttg
     3061 gtactttaga ggctcgtagg gataaggcaa ttaagctatt atttgctaaa caagaggcag
     3121 agcggatagc taaggaaaaa tctgattttc taaaagcgaa caaaaaagag gatgcgatta
     3181 gtgagcctag caagaagcgt ggaagacaga ttattcaaat gactgatgat ggaacaataa
     3241 taaaagaatt cgatactata gcttctgctg taaaagaagt tggcgttaac tcaaaaagta
     3301 ttagggatgc tgcaaatgga gttcaaaaac atgctggagg atactactgg aaatacaaag
     3361 attagcagat aatctaagat aacctaggat cttcttaata gatacccgtg gtgctagtta
     3421 attttaatat cctaaagaat atctcaaagt tccttcaaaa cgtcgttgtg ctcgacttaa
     3481 tagaacactg taagccagta atatgctttc aaatctactc tctagccctt taacagaacg
     3541 tgagttgaaa ttttgacaac ctaatttttc taaggaagaa aaaacagtcc caactgtttt
     3601 tctttgtttt ccaagtaagc tgtggtctag tttttctgat ttatccatgt ttttacgtgg
     3661 cggaa
//
LOCUS       DAZKLM010000152         3632 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3764
            SAMEA115706913-rid22582563.denovo.158, whole genome shotgun
            sequence.
ACCESSION   DAZKLM010000152 DAZKLM010000000
VERSION     DAZKLM010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706913
            Sequence Read Archive: ERR13196647
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3632)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3632)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287823.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287823.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,004
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,849
            CDSs (with protein)               :: 2,849
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 41 of 96
            Pseudo Genes (incomplete)         :: 52 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 15 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3632
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706913-rid22582563.denovo.158"
                     /isolate="VRE3764"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(249..926)
                     /gene="nth"
                     /locus_tag="ACG842_002684"
     CDS             complement(249..926)
                     /gene="nth"
                     /locus_tag="ACG842_002684"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HFQ0249162.1"
                     /translation="MLSKQKTMEALETMYGMFPEAHGELKHNNPFELLIAVILSAQAT
                     DVSVNKATPDLFASFPTPDALAEASIDEIILKIKTIGLYRNKAKNIKACAQQLIERFD
                     GQVPTSREELMSLPGVGRKTANVVLGDAFGIPAIAVDTHVERVSKRLRICKLDATVME
                     VEETLMRKVPQELWVKTHHTLIFFGRYHCTARNPKCEVCPLLSICQDGKNRMRLKEKA
                     LKKKPRL"
     gene            complement(933..1631)
                     /locus_tag="ACG842_002685"
     CDS             complement(933..1631)
                     /locus_tag="ACG842_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HFQ0249163.1"
                     /translation="MLNLENYLSAGQTVISNLLLKNYHRIGLQSEEFLFVLQLHMAQL
                     EGDTFPDLQMIAMDMGIKQDQIFQILDRLVTNGFIKIETTIVNGKKADRYNLYPIYDL
                     LGEYLKTQEKKYEEKTQEKEIQSVYQLFEQEFGRPLSSIEFQRIGQWLEEDHYQPEIL
                     KLALREAVLNQAYSFNYVDRILLSWERKNLRTKQQVEEDQKRRKQQLLQREVEQSAQQ
                     EELPKVSLKNWLEE"
     gene            1899..3083
                     /locus_tag="ACG842_002686"
     CDS             1899..3083
                     /locus_tag="ACG842_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance MFS efflux pump"
                     /protein_id="HFQ0249164.1"
                     /translation="MYSFTNKHSKVFGYVSVLFTGLGFTIVSPVLPFLTLPYSHSIHQ
                     QAFYITLLMSVYALAAFLSAPILGSLSDHFGRHPILIISLLGSSIGYLLFGLGNSIWM
                     LFLGRIIEGLTAGEISTLYAYFADITEPNERTKVFGWMGALVGIGTTLGPIIGGLLAE
                     LGNSVPIFIGALFTFLNAVYGYTFMLESLPIKKRSVDLSFSHVRPFHQLQQLFKISSV
                     IPLLTAGFAVWLAAGSLQSIFSQFSIDAFQWKAGLVGLSFSLIGILDTVSQLFIMPRL
                     LKKFSEQQITRIGMFSEILAYLFITLSGILLLPILFLFGIICYGFGDSIFTPVFNGQL
                     SNSVSDNQQGLVMGGTQSIQSLSRVIGPLIAGQLYAAAPFLPTVFGFLLMIFAYYHYN
                     KK"
     gene            complement(3089..3532)
                     /locus_tag="ACG842_002687"
     CDS             complement(3089..3532)
                     /locus_tag="ACG842_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HFQ0249165.1"
                     /translation="MDKRQLLIEEYLDLANEIIHRNKPEMEKEFAGFTLNELEVIEHI
                     GKIPDPNVTKLANASYMTRGAISKLTKKLIARGIINTYQSEENKKEIYFQLTPIGQKI
                     RNRHEQLHQKWVQRDATVFENMSKEEFDTVFRFIGRYREFIRSSK"
BASE COUNT          940 a          724 c          608 g         1360 t
ORIGIN      
        1 tatttatata atcctttgga atactggact tattttatag gtatattgga aggcattatt
       61 ttctacatcg ggtattttcc caataaaaat aacaactttt atttttatcg caataaattg
      121 taataagtta ccatcctagc tactgagtat gtacatttac tttttcatgc aaaaaagata
      181 aacgaaacag cttttctcca taataacaag ataattcata catttatttg caattaaaga
      241 aaccacgtct acaggcgtgg tttctttttt agcgcctttt cttttagacg catccgattt
      301 ttcccatctt gacatattga tagcaaagga catacctcgc acttgggatt tcttgccgtg
      361 caatgatatc taccaaaaaa aatcagtgta tgatgtgtct ttacccataa ttcttgtggt
      421 actttccgca tcagtgtttc ttctacttcc atgaccgtag catctagttt gcagatcctc
      481 aggcgttttg agacacgttc cacgtgggta tctactgcga ttgctggtat cccgaatgca
      541 tcccccaata cgacatttgc tgtctttctg cctacaccag gaagagacat caattcttct
      601 cttgacgttg gcacttgccc atcgaaacgt tcaatcagct gctgtgcaca cgctttgatg
      661 ttttttgctt tatttcgata caaaccaatc gtttttattt taagaatgat ttcatcgatt
      721 gaagcttctg ctaacgcatc gggtgtcgga aaagaggcaa acaaatctgg cgttgcttta
      781 ttcactgata catctgtcgc ctgggcactt aagatcactg caatcaataa ctcaaatgga
      841 ttattgtgct tgagttctcc atgtgcttcg ggaaacattc catacatcgt ttctagagct
      901 tccattgttt tttgtttgct taacatattc cgctactcct ccaaccaatt tttcagtgag
      961 actttcggca attcttcttg ttgagctgac tgttctactt ctctttgaag aagctgctgt
     1021 tttcttcgtt tttggtcttc ttccacttgt tgttttgtac gtagattttt tctttcccac
     1081 gatagcaaaa tgcggtcaac gtaattaaaa ctataagcct gatttaatac ggcttcccgc
     1141 agtgctagct tcaaaatttc cggctgataa tggtcttctt ctagccattg tccgatccgc
     1201 tggaactcga tcgaagaaag cgggcgtcca aattcttgtt caaataattg atacactgac
     1261 tggatttctt tttcttgtgt tttttcttcg tatttttttt cttgtgtttt taaatactca
     1321 cctaatagat catagatggg gtaaagatta tatcgatctg cttttttacc gttgacaatc
     1381 gtcgtttcta tcttgataaa tccatttgtt acgagacgat ccagtatttg aaaaatctga
     1441 tcttgtttga ttcccatatc catagcaatc atctgcaaat cagggaaagt atctccttca
     1501 agctgtgcca tatgcaactg tagaacaaat aaaaattctt cagattgtag accgatccta
     1561 tgataatttt taagtaaaag attagatatg actgtttgtc cagcgcttaa gtaattttct
     1621 aaattcaaca tgatttctcc tccgctcaat cacagtatac gtaaaaaaaa catgggaagc
     1681 aagaacaggt ttcttttcta tttacttcac tgatcggtca gatttacaat gtcgtctact
     1741 tgatttttgt ttgttcatgt ttttgatgcg tatcttcact tttcggaata aataaattaa
     1801 gtagatttcg ctgttcctta gaatgttgac aaggaaacaa aattactata cactattttt
     1861 gtttccaagt taacattatt gcataaagga gtttttacat gtacagtttc acaaataaac
     1921 attctaaagt ttttggatac gtctctgtct tattcactgg attaggattt actatcgtca
     1981 gtcctgtact tccgttttta acgctgcctt attctcactc catacatcag caagctttct
     2041 atatcacttt gctcatgtct gtttatgctc tagctgcctt cttatctgcc cccattctcg
     2101 gtagcttaag tgatcatttt gggcgtcatc cgatccttat tatcagttta ttaggatcaa
     2161 gtatcggata tttgcttttt ggtttaggaa attcgatatg gatgttgttc cttggtcgta
     2221 tcattgaagg attgacggca ggagaaatct ccaccttata tgcttacttt gccgacatca
     2281 cggaacctaa tgaaagaacc aaagtttttg gatggatggg tgctttagtc ggtataggaa
     2341 caacgctggg tcctattata gggggtctat tagctgagtt ggggaattct gttcctatat
     2401 ttattggagc actctttact ttcttaaatg ccgtttatgg ctacacattt atgctagaaa
     2461 gccttccaat aaaaaaacgt tcagtagatt taagtttctc ccatgtaaga ccttttcacc
     2521 aattacaaca attattcaag atttcatccg ttattccttt attgacagca ggttttgctg
     2581 tttggttggc agctggctct cttcaatcta tcttttctca attttcgatt gacgcctttc
     2641 agtggaaggc tgggttggtt ggtttaagct tctctttgat tggaatcttg gatactgtct
     2701 cgcaattgtt cattatgcct cgattgctca aaaaattttc agaacagcag atcacaagaa
     2761 tagggatgtt cagtgaaatc ctcgcctatc tattcatcac attatctgga attcttctac
     2821 tgccgatttt gtttttattt gggattatct gttatggttt cggtgattct atttttactc
     2881 ctgtattcaa tggccaacta tccaattctg tctcagataa ccagcaaggt cttgtaatgg
     2941 gcggtaccca aagtatacaa tctctatcgc gcgtcatcgg cccgctaatc gcaggacagc
     3001 tttatgctgc agcaccattt cttccgacgg tattcggatt tttgcttatg atttttgcct
     3061 attatcacta caataaaaaa tagtagcttc atttgctact tcgtataaat tctctgtatc
     3121 gaccgataaa acgaaatact gtgtcaaatt cttcttttga catattttca aatacagtag
     3181 catctcgctg tacccatttt tgatggagtt gctcgtgtct attccttatt ttttgtccga
     3241 ttggcgtcaa ttgaaaatag atttcttttt tattctcttc gctttgataa gtgtttatga
     3301 tccctctagc tatcagtttt tttgttagct tactgatggc tcctctagtc atgtaactag
     3361 cattcgctaa ttttgtcacg ttaggatcag ggattttccc gatatgttca atcacttcta
     3421 gttcatttag agtaaatcct gcaaattctt tttccatttc tggtttatta cgatgaataa
     3481 tctcattagc taaatccaga tattcttcta ttagcagttg tcttttatcc atttctacac
     3541 cttctttttc tttcattata ttgttttttt gtttcctatt caacaaagat aaaaatattt
     3601 tgtaggaaaa cccaatctga aaccaagaaa aa
//
LOCUS       DAZKLM010000153         3602 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3764
            SAMEA115706913-rid22582563.denovo.159, whole genome shotgun
            sequence.
ACCESSION   DAZKLM010000153 DAZKLM010000000
VERSION     DAZKLM010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706913
            Sequence Read Archive: ERR13196647
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3602)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3602)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287823.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287823.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,004
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,849
            CDSs (with protein)               :: 2,849
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 41 of 96
            Pseudo Genes (incomplete)         :: 52 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 15 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3602
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706913-rid22582563.denovo.159"
                     /isolate="VRE3764"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..186
                     /locus_tag="ACG842_002688"
     CDS             <1..186
                     /locus_tag="ACG842_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFQ0249166.1"
                     /translation="VEDASLSGENERINELIEFIRKNKFRTLEYDDKLVRKIIQSVTV
                     YEGHFVIAFKSGIEMEI"
     gene            329..553
                     /locus_tag="ACG842_002689"
     CDS             329..553
                     /locus_tag="ACG842_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018579902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0249167.1"
                     /translation="MTTAEMIKELCEQMNISVSELARRIGQTPQNFNKKLQRETVTLD
                     ELKAIADVLGVKFVQAFILPDGEEIKISNE"
     gene            568..1437
                     /locus_tag="ACG842_002690"
     CDS             568..1437
                     /locus_tag="ACG842_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HFQ0249168.1"
                     /translation="MVGNVIQIVTEKLSSLPFIEGIVLGGSRARGTHTENSDIDIGIY
                     YNSDSFDLTAINQIATELDDKNRNNLVVPPGAWGDWINGGGWLFINGYHVDLILRDIK
                     RVEQIIKDTEQGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAEIY
                     PTALKKSLMNFFIFEAEFSLMFVKANAGVEDKYYIAGHVFRIISCLNQVLFACNNAYC
                     INEKKAIKLLETFEYKPEKYAERVNHIFEVLGLSLFECYDMTEKLYKEVNEIVSEINN
                     FLNEESSDERKQI"
     gene            1418..2152
                     /locus_tag="ACG842_002691"
     CDS             1418..2152
                     /locus_tag="ACG842_002691"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008425 -
                     2-polyprenyl-6-methoxy-1,4-benzoquinone methyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0061542 - 3-demethylubiquinol-n
                     3-O-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0102208 - 2-polyprenyl-6-hydroxyphenol
                     methylase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFQ0249169.1"
                     /translation="MKENKYDDNIFFQKYSQMSRSQKGLAGAGEWETLKKMLPDFKGK
                     RVLDLGCGYGWHCIYAMENGASSVVGVDISHKMLEVAKGKTHFPQIEYECCAIEDVDF
                     PEESFDVILSSLAFHYVADYENLIKKIYRMLKAGGNLVFTVEHPVFTAHGTQDWYYNE
                     KGEILHFPVDNYYYEGKRTAMFLEEKVTKYHRTLTTYLNTLLSNSFIINQIVEPQPPE
                     NMMDIPGMADEMRRPMMLIVSAKKKM"
     gene            2185..3093
                     /locus_tag="ACG842_002692"
     CDS             2185..3093
                     /locus_tag="ACG842_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase ANT(6)-Ia"
                     /protein_id="HFQ0249170.1"
                     /translation="MRSEKEMMDLVLSLAEQDERIRIVTLEGSRANINIPKDEFQDYD
                     ITYFVSDIEPFISNDDWLNQFGNIIMMQKPEDMELFPPEEKGFSYLMLFDDYNKIDLT
                     LLPLEELDNYLKGDKLIKVLIDKDCRIKRDIVPTDIDYHVRKPSAREYDDCCNEFWNV
                     TPYVIKGLCRKEILFAIDHFNQIVRHELLRMISWKVGIETGFKLSVGKNYKFIERYIS
                     EDLWEKLLSTYRMDSYENIWEALFLCHQLFRAVSGEVAERLHYAYPEYDRNITKYTRD
                     MYKKYTGKTGCLDSTYAADIEERREQ"
     gene            3090..3570
                     /locus_tag="ACG842_002693"
                     /pseudo
     CDS             3090..3570
                     /locus_tag="ACG842_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
BASE COUNT         1251 a          521 c          789 g         1041 t
ORIGIN      
        1 gtagaagatg cctccctcag tggcgagaac gagcgaatca atgagctgat tgaatttatc
       61 cgcaagaaca aattccgcac cttagaatat gatgataagc ttgtaaggaa gataatccag
      121 agcgttacag tctatgaagg ccacttcgtc atagccttca aatctggcat cgaaatggaa
      181 atatgaaaac cagaagtaaa tagcccatga ctctacagta gagttgtggg ttttcttttg
      241 cttgctgatt ataataatat tttaataact attgtgttta tcaaccaaat ggtttataat
      301 attcttagcg taaacttgga ggtgctgtat gactacggca gaaatgatta aagaactgtg
      361 tgagcaaatg aatataagtg tttccgaact tgctagacgt attggccaga ctccacagaa
      421 tttcaataaa aaattacaac gagaaacagt aaccttggat gagttgaagg ccattgctga
      481 tgtgctaggt gtcaagtttg tgcaggcatt tattttacca gatggagaag aaataaaaat
      541 atctaacgaa taaaggagga tgtttatatg gtaggtaatg ttattcaaat agtaacagag
      601 aaattatcat ctttgccttt tatagaaggc atcgtattag ggggctcacg tgcaagaggc
      661 acccatacag aaaattctga tatagatatc ggaatctatt acaattcaga ctcatttgac
      721 ctgacagcga ttaatcaaat tgctacagaa ttggatgata aaaatagaaa caaccttgtt
      781 gtgcctcccg gagcgtgggg tgattggatt aatggcggcg gatggttatt tataaatggg
      841 tatcatgttg acttgatttt acgtgatata aaacgggtag aacaaataat caaagatacg
      901 gagcaaggaa ttgttactgc caattatcag accgggcatc cccatggtta tataagtgca
      961 atgtatcgtg gagaattagc gattagtaaa atactatatg ctaagaacga aagcttatgc
     1021 gaattaaaaa agcaggcaga aatttaccct actgctctaa agaaaagctt gatgaacttt
     1081 tttatatttg aagctgaatt ctctttaatg tttgtaaaag caaatgcggg ggtagaggat
     1141 aaatattata ttgcaggcca tgtttttcgt attatttcat gcttaaatca agtactattt
     1201 gcatgtaata atgcttattg cattaacgaa aagaaagcta taaaactgct tgaaactttt
     1261 gaatataaac ctgaaaaata tgccgaaaga gttaatcata tttttgaagt actcggtctt
     1321 tctctttttg aatgctacga tatgaccgag aaactttata aagaagtgaa tgaaattgta
     1381 tcggagataa ataacttttt aaacgaggag agttcagatg aaagaaaaca aatatgatga
     1441 taatatattt tttcaaaaat acagtcaaat gagtcgctcg cagaaaggac tggctggtgc
     1501 gggagaatgg gagactttga aaaagatgct acctgatttt aagggtaagc gtgtgcttga
     1561 tttaggatgc ggctatggat ggcactgtat atatgcgatg gaaaacggtg cttcctctgt
     1621 agtaggtgtt gatatttctc ataaaatgct cgaagtagca aaaggaaaaa cccattttcc
     1681 acagattgaa tatgaatgct gtgccataga agatgtggat ttcccagagg agagctttga
     1741 tgtaatacta agttcgcttg cgtttcatta tgtagcagac tatgagaatt taataaaaaa
     1801 gatatatagg atgctgaagg ctggtggcaa tttagttttt acagttgaac atcctgtttt
     1861 tactgctcat ggaacacaag actggtatta taacgaaaaa ggagaaatac tgcatttccc
     1921 ggtggacaat tattattatg agggcaaacg gacagctatg tttttggaag aaaaggttac
     1981 aaaatatcat agaacactga ccacatatct aaatacactg ctttcaaata gttttataat
     2041 aaatcagatt gtggagccac agccgccaga gaacatgatg gatattccgg ggatggcgga
     2101 tgaaatgcga cgcccaatga tgctgattgt atcggcaaaa aagaagatgt aataatatag
     2161 aaaaaataaa cgaggagtat gtaaatgaga tcagaaaaag aaatgatgga tttagtactt
     2221 tctttagcag aacaggatga acgtattcga attgtgaccc ttgaggggtc acgcgcaaat
     2281 attaatatac ctaaagatga atttcaggat tatgatatta catattttgt aagtgatata
     2341 gaaccgttta tatctaatga tgactggctt aatcaatttg ggaatataat aatgatgcaa
     2401 aagccggagg atatggaatt attcccacct gaagaaaagg gattttccta tcttatgcta
     2461 tttgatgatt acaataaaat tgatcttacc ttattgccct tggaagagtt agataattac
     2521 ctaaagggcg ataaattaat aaaggttcta attgataaag attgtagaat taaaagggac
     2581 atagttccga ctgatataga ttatcatgta agaaagccaa gcgcaaggga gtatgatgat
     2641 tgctgcaatg aattttggaa tgtaacacct tatgttatta aaggattgtg ccgtaaggaa
     2701 attttatttg ctattgatca ttttaatcag attgttcgcc atgagctgct gagaatgata
     2761 tcatggaagg tcggcatcga aacaggcttt aaattaagtg taggcaagaa ctataagttt
     2821 attgaaaggt atatatccga ggatttgtgg gagaaacttt tgtccaccta ccggatggat
     2881 tcctatgaaa acatatggga agcattattt ctatgccatc aattgttcag ggcggtatcc
     2941 ggtgaggtgg cggaaaggct tcattatgcc tatccggagt atgataggaa tataacaaaa
     3001 tataccaggg acatgtataa aaaatacact ggtaaaaccg gctgcctgga tagcacatat
     3061 gccgctgata tagaagagag gcgggaacag tgattacaga aatgaaagca gggcacctga
     3121 aagatatcga taaacccagc gaaccatttg aggtgatagg taagattata ccgaggtatg
     3181 aaaacgagaa ttggaccttt acagaattac tctatgaagc gccatattta aaaagctacc
     3241 aagacgaaga ggatgaagag gatgaggagg cagattgcct tgaatatatt gacaatactg
     3301 ataagataat atatctttta tatagaagat atcgccgtat gtaaggattt cagggggcaa
     3361 ggcataggca gcgcgcttat caatatatct atagaatggg caaagcataa aaacttgcat
     3421 ggactaatgc ttgaaaccca ggacaataac cttatagctt gtaaattcta tcataattgt
     3481 ggtttcaaaa tcggctccgt cgatactatg ttatacgcca actttgaaaa caactttgaa
     3541 aaagctgttt tctggtattt aaggttttag aatgcaagga acagtgaatt ggagttcgtc
     3601 tt
//
LOCUS       DAZKLM010000154         3490 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3764
            SAMEA115706913-rid22582563.denovo.160, whole genome shotgun
            sequence.
ACCESSION   DAZKLM010000154 DAZKLM010000000
VERSION     DAZKLM010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706913
            Sequence Read Archive: ERR13196647
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3490)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3490)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287823.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287823.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,004
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,849
            CDSs (with protein)               :: 2,849
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 41 of 96
            Pseudo Genes (incomplete)         :: 52 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 15 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3490
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706913-rid22582563.denovo.160"
                     /isolate="VRE3764"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..2232)
                     /locus_tag="ACG842_002694"
     CDS             complement(<1..2232)
                     /locus_tag="ACG842_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HFQ0249171.1"
                     /translation="MRNKFHKLLSMFLVMLIVINQFGNITVLAESLAEQPERSLTIEV
                     DQEEAALGEQFQLRVRGTPDLLNALVIDKSEDLIEGEVKQLNDYERLITYSSQHSGKY
                     ELTARSGDIQSNTVSIEISVPEESTSESFNVPEESTSESFNIPEESLSESFNEARSSE
                     PSITQESQIERQEELLNYDIGISAERPTLPSRHTQTLKGYIQGGAGDILEEYGEMVVR
                     VPRASIGLIEFIRVEVADDSPLKPLAFYQEGTDVVFPFTFDYEGLNEAAQSYTFSITF
                     EVAFFQSGGTEPNNDFQVSLTYAERAANTEFSITPIPSSRPIFSKLYYGSREIDSQGN
                     LGNLLIDGQDVTNSNKFILQVNYEGSRKDGVVVTDVLPQGTTLAPNPTTWSSTGNREP
                     ISGGVRIAEVTNPGEANVRTHYVSEEFVDKVSFNKETNTLVVDFGNLAEGQSYFIEYA
                     LEVTDVDLFNEGRQEDGFSRNRASMTYNNQVMSRDYRLRIPESNLPAISLRKSVDKNV
                     INLNENELTYTLHVSMFSGTRNTLVIEDVLEEGMEFVSFLSDIPSFIDAEVNNGIVRF
                     SANNTIGGPTSFTLSFKVNVEAYAVGDQILNFATLVNSDNRLNTSTVRTRKIDGRIKV
                     IKTDENGQRLAGAKFEILSSDSQVIQEGITAENGEFLSEPLPVGSYQVRETEAPEGYL
                     LDATVHDVFINEYSIEEVTLTIENILDTGGVELIKHAEGSGEVLQGAVFELQNREGET
                     LQTG"
     gene            complement(2904..3172)
                     /locus_tag="ACG842_002695"
                     /pseudo
     CDS             complement(2904..3172)
                     /locus_tag="ACG842_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720767.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS607 family transposase"
BASE COUNT          983 a          772 c          549 g         1186 t
ORIGIN      
        1 accggtttgt agcgtttctc cttctcggtt ctgtaactca aaaacagctc cttgaaggac
       61 ttcaccactg ccttcagcat gtttaattag ttcaacacca cccgtatcaa ggatgttttc
      121 aatggttaac gtcacttctt ctatggagta ttcatttata aaaacatcat gtaccgtcgc
      181 atctaaaaga tacccttccg gtgcttctgt ttctcggact tgatagctac ctacaggaag
      241 cggttcactt aaaaattctc cgttttctgc cgttatacct tcttgtatca cttgtgaatc
      301 gcttgataaa atttcaaatt ttgctcctgc cagcctttgg ccattctcat cagttttaat
      361 tactttaata cgcccatcga ttttccttgt tcttacagta ctcgtattca atcgattgtc
      421 actattcaca agcgttgcaa agtttaaaat ttgatcgccg accgcatacg cctctacatt
      481 gaccttaaaa gagagggtaa aacttgtcgg accaccaatc gtattattcg cactgaaacg
      541 aactattcca ttattgactt cggcatcaat gaaagaggga atatctgata gaaatgacac
      601 aaattccatg ccttcttcca aaacatcttc aataacaagc gtatttcttg ttccagaaaa
      661 catcgagaca tgcagagtat acgttagttc attttcattg agattgataa catttttgtc
      721 cacacttttt cttaaagaga ttgccggaag attactttca gggattctca agcggtagtc
      781 tcggctcatt acctgattat tgtaggtcat actcgctcga ttccgagaaa acccatcctc
      841 ttgtcttcct tcattgaaca agtcgacatc ggtcacttca agagcatatt caataaaata
      901 cgattgaccc tctgctagat taccaaagtc cacaacaagt gtattcgttt ccttattaaa
      961 agatactttg tccacaaatt cctcggaaac ataatgagtt ctaacgtttg cttcacctgg
     1021 attcgttact tctgcgatcc gaaccccacc tgaaataggt tctctatttc cagtggaact
     1081 ccatgtagtc ggattcggtg ccaaagttgt tccttgcggt agtacatcgg taaccactac
     1141 accgtctttt cgtgatcctt cataatttac ctgcaaaata aatttattac tgttggtcac
     1201 gtcttgcccg tcaatcagca gattcccaag atttccttgg ctgtcgattt ctctagagcc
     1261 atagtataat ttcgaaaaaa tgggtcttga tgaaggaata ggcgtaattg aaaattccgt
     1321 gtttgctgct cgctcagcat aagtaagact cacttgaaag tcattatttg gctcagtacc
     1381 gcctgattga aagaaagcaa cttcaaacgt gatactgaat gtatacgact gagcggcttc
     1441 atttaatcct tcataatcaa aggtaaatgg aaaaacaacg tctgtgcctt cttggtaaaa
     1501 tgccagtggc tttaaaggtg aatcatcagc gacctccact ctaatgaact caattaagcc
     1561 aatcgatgct ctaggaactc gtacgaccat ctccccatat tcttcaagaa tatcgccagc
     1621 gcctccttga atgtatccct ttaacgtttg tgtatgcctg ctcggcaaag tggggcgctc
     1681 agcagagatg ccgatgtcat aattcaagag ctcctcttgt ctttcaatct gagattcctg
     1741 cgttatagat ggctcagatg atctggcttc attaaaagat tcacttaagc tttcttctgg
     1801 tatattaaaa gattcacttg tgctttcttc tggtacatta aaagactcac ttgtgctttc
     1861 ttctggtaca ctgatttcta ttgagaccgt atttgattga atatcaccgc ttcttgcggt
     1921 caattcgtat ttacctgaat gctgggaaga ataagtaatc agacgttcgt aatcattgag
     1981 ttgcttcact tccccttcaa tcaaatcctc actcttatca atgaccaatg catttagcag
     2041 gtctggtgtt cctctgactc gcaactggaa ctgctcaccc aaagcagcct cctcttgatc
     2101 aacttcgatc gtcagcgaac gctctggctg ttctgctaaa gactcagcta gaaccgttat
     2161 gttcccaaat tgattgatca caataagcat aactagaaac atacttaaca gtttatgaaa
     2221 tttgttcctc actattactc ccccttataa ttttgactaa acaacaaggt acccgataat
     2281 tttctaaaaa gattccccaa ctcaaaatgg ctgcgttacc tccctcgtat tttcggttca
     2341 actcaagcgt tgcaatgtaa gctgtaaaca aagctaatga ttgccgattg tcttctgttg
     2401 attgattcga tagctttgta tgacttttag ttgtccgtgt ggcttaacca taattatgat
     2461 ttcacgattc ctcctgccag tgccttgcac aagctggagc gtaaccctaa tagttatttt
     2521 gttaatgtac ttatatactt ttatactttt ccattagaaa caatcactaa atcgttgata
     2581 ccaaggtcag actctagaat ttcatcagtg agtccctgta ttggttaacc ttcatcaatc
     2641 ccaaaaaaat atatcaatgc ttagcatcaa aatgaatata tagtttagaa tacttgggat
     2701 tcatcgataa ctgatcgctt atttggccat attatttggc acttggttca actaaacgca
     2761 tttaggatgt tccttcattc tcgtcatgga tgttctcaaa ccgtcattac taataaactg
     2821 tccaccgaat cgatggttca tttcttgcat tttccacgtc caattactct ttttgctctt
     2881 tatttgggcg tagtttcact ttcttaactt gtatcgtctt ccatcaactc cttaatcatc
     2941 ttcttagctt tattggctcg gtttccttgt aaaaggtatc tacaaatagt gacaatctgt
     3001 attaaatctt catcattttc tgtaatgtca ataatttcta tattctgtac catatattgc
     3061 aaagggtttc actaagttca taaccgaagc gaattaaaca acttttgtat agaataacta
     3121 ccttttctat ttttccattg gtaattctat ctattaattg gtttaatcct tttgttgtag
     3181 ttgattccac taccaagatg attctctcga attttaaagc ttgaagatgt attgctattt
     3241 ttgctaactg gcgtatttgc aactattttt cccttctatt cttccttcat tgattcccct
     3301 ctaaaattac tatccataaa aaattgcata aatgacaata gtatttatat tagtcatact
     3361 gctttttaac acagcaaatt ccagaaaaac attcgttaca tatttgtgaa tttttccaaa
     3421 aaataacatt tttataaaac gacgaaaaat aaatcactac cagtcaactt ctataatatt
     3481 accaaataac
//
LOCUS       DAZKLM010000155         3200 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3764
            SAMEA115706913-rid22582563.denovo.161, whole genome shotgun
            sequence.
ACCESSION   DAZKLM010000155 DAZKLM010000000
VERSION     DAZKLM010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706913
            Sequence Read Archive: ERR13196647
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3200)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3200)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287823.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287823.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,004
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,849
            CDSs (with protein)               :: 2,849
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 41 of 96
            Pseudo Genes (incomplete)         :: 52 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 15 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3200
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706913-rid22582563.denovo.161"
                     /isolate="VRE3764"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            301..585
                     /locus_tag="ACG842_002696"
     CDS             301..585
                     /locus_tag="ACG842_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0249172.1"
                     /translation="MDILVRNIDPKFVATIDKRCQELTNKTGEKWSRNKYLKVLIESD
                     FDRQLTTYKKDQFDLLLDKFIAIQTENTQILSEYVYTNNQIIEMLIGGMI"
     gene            588..1004
                     /locus_tag="ACG842_002697"
     CDS             588..1004
                     /locus_tag="ACG842_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0249173.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIEKNPDINNYSEAIRFLCMSY
                     EPDTNTTTTSNQAKLNAMGKEISIMTEIIAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     EAKLNPSNRNRPKNHQKETSQTVTSRNFDPTNFLKR"
     gene            complement(1141..1860)
                     /locus_tag="ACG842_002698"
     CDS             complement(1141..1860)
                     /locus_tag="ACG842_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFQ0249174.1"
                     /translation="MDEKTELTVKYQNELNLVPLKNFNAKQMDLFFALCARMKDRGLK
                     KVRFSFEELKELSDYKMTATKAFVGDLEKLYKDMLNLTYRTEDEDSIRYFVLFTGFEI
                     NKTKKFVEVSVNPDLEYIINGLTAEFSRFELSAFTSLRSTYAKTLFRLLMQYRSTGYY
                     VVRIEEFRSLMDIPEYYQMGNIDQKVLNPALRELKKYFNDLKVTKIKAKKGNKIAKLE
                     FTFSSLTSDLPKISLRNWLEE"
     gene            2522..2608
                     /locus_tag="ACG842_002699"
     CDS             2522..2608
                     /locus_tag="ACG842_002699"
                     /inference="COORDINATES: protein motif:HMM:NF033608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HFQ0249175.1"
                     /translation="MFTNFICSLLVGLLSALFSYWLNNRNKK"
     gene            complement(2911..>3200)
                     /locus_tag="ACG842_002700"
     CDS             complement(2911..>3200)
                     /locus_tag="ACG842_002700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0249176.1"
                     /translation="EYRRQRSDFISRKIVRLEKEKMEKFPYEYFQNLEKLEQAIQAIK
                     FDLTDEKLLEYKNMTPERKRSLEKLMQLVNEDGGISDAAFDAWSEFHGEYN"
BASE COUNT         1065 a          597 c          499 g         1039 t
ORIGIN      
        1 aaaaaaaatg tcgtttgaaa aactaaataa atttttgatc gtgcattttt aaatttggtt
       61 atcgattaca cgtgcagcac tcccccccga attttaaaga gcataaggaa acgtgtaccc
      121 actttctaaa aaaagtgggt agataggctg tgcctatcac gtttctcaga aaaaaatttg
      181 atatcacttt ttcgccatcg tgggtagctt atgtatagca ttttggcaaa aaacagctat
      241 acaaatatag tatttgtatg gtatacataa attatacata attgcaaaag gagatttttt
      301 atggacattt tagttcggaa tattgatcca aagtttgttg caacaataga caaacgttgc
      361 caagaactca cgaataaaac aggagaaaaa tggagtcgaa ataaatatct aaaagtttta
      421 atcgaaagtg attttgaccg tcaacttacg acttacaaaa aagaccaatt cgatttattg
      481 ctcgataaat ttattgctat ccaaacagaa aatactcaaa tattaagcga gtatgtctac
      541 acaaataatc aaatcattga aatgcttatc ggaggaatga tataaccatg agaaaaactt
      601 ctgtccaaaa acatgttact ttgtacccta gtcatgttga cgttttagat tccatcatcg
      661 aaaaaaatcc agatatcaat aactactcgg aagccattcg ctttttatgt atgagttatg
      721 aacccgatac caacacgacc actaccagca atcaggcaaa actaaatgct atgggaaaag
      781 aaatttccat catgactgaa atcatcgcag aatttgctga tttatatcta aaagatacag
      841 gcgttcttat tggcacaaat tctaaagtat atcaagaagt gaaagaaaaa atcgaagcta
      901 aactaaatcc atcaaatcga aatcgaccaa agaatcatca aaaagaaacc agtcaaacag
      961 tcacttctag aaatttcgac ccaacaaatt ttctaaaacg ataaaaaaca gataggagtt
     1021 attcctatct gttttttatt tagaagcccc atacaatatg ctagaatgta ctagcaatct
     1081 tagtcggttg taagggactt gtctacttgc ttaatgtaag gggggcgagt tcttttttat
     1141 ttattcttct aaccagttac gcagagaaat ctttggcaaa tctgacgtta ggcttgaaaa
     1201 agtaaattct aatttggcga ttttattgcc cttttttgct tttatttttg tgactttaag
     1261 atcattgaag tattttttta gttctcgtaa tgcaggattt aatacttttt gatcaatgtt
     1321 acccatttga tagtattctg ggatatccat taagctccta aactcttcaa tacgaactac
     1381 ataataacca gtagaacggt actgcataag cagtcggaat agagtttttg catacgtaga
     1441 acgcaacgac gtgaaagcag ataattcaaa tctactaaat tcagctgtta aaccattaat
     1501 aatatattct aggtccggat taacactgac ttcaacaaac ttttttgttt tattgatttc
     1561 aaaaccagta aacaaaacaa agtagcgaat agaatcctca tcttcagtac ggtaagtaag
     1621 attaagcata tctttataga gcttttctaa atcaccgaca aaggcttttg tagcagtcat
     1681 cttataatca cttaactctt ttaattcctc gaaagaaaat ctaacttttt tcaaaccacg
     1741 atctttcata cgagcgcata atgcaaaaaa taaatccatc tgcttagcat tgaaattttt
     1801 cagcggtact aaattcaatt cattttgata tttgactgtt aattccgttt tttcatccat
     1861 tcctttcttc cccccttaca aataaataat aacctttaac aaccaactcg taaataggtt
     1921 ggttgttatt atacaaaagg atattagtac tcagataaaa agttgttagt actcagataa
     1981 aaagttgtta gtactcagat aaaaagttgt tagtactcag ataaaaagtt gttagtaccc
     2041 ttctgtatcc gttgggagag taaggctcaa ctgcccccta aaaagggttc taaaaaatct
     2101 ttaaaaaata aataaaataa acgcgcgcgc gcgagcaaaa tttcccgcca aaaaatcacc
     2161 cactttttaa aataaacagg ttcgcgcatg atattaaact gccaatctag ctccctatgg
     2221 tcgctgtaag aaggagtgat cgctacgctc tcacatttta cttgcagcag tcgcctttag
     2281 gctccttcgc caaaaataat tcattaggga ctcctttgtg gcgtcctgag agctcgtcta
     2341 agccgttcta agacaagatt cgataaaaac tatgtccttc gttctaaagc gccttacagg
     2401 cggtcagggc aagccctaac aaccccaagg caataaaaaa cttaggctta tttttgcttt
     2461 tttattgtat aattgattgt cgcctatccg acagtcttgc gactggaagg aggtgtgttt
     2521 cgtgttcact aactttattt gctcccttct tgttgggctt ttaagcgctt tgttcagtta
     2581 ctggttgaat aaccgaaaca agaagtaaaa cttaattaag gcgactcagc ccacaaaaaa
     2641 agccccagag aatccgacct ctctggggct ttcgtgtgtt tcgtgttcac ttatttgcaa
     2701 tcaaattata acagaaataa atatttattt caaaaatatc ctacaaaagt ctaaaatccg
     2761 taattatttc ttatatgcaa catttacaaa taaagtcgct ctgagagctc gtatttttcg
     2821 atttaagacg ttttatttag tttttggtgt attttcattg gtccaagcta aaaaactctt
     2881 agaagcgatt ttagagccat ttaaacccac ttaattatat tctccatgaa attcgctcca
     2941 tgcgtcaaaa gctgcgtctg aaattccgcc gtcttcgttc actagttgca ttaatttttc
     3001 caacgatcgt tttctttcgg gtgtcatatt tttatattcc aacaattttt cgtctgttaa
     3061 atcgaacttg attgcttgaa ttgcttgctc taatttttct aagttttgaa aatattcgta
     3121 aggaaatttt tccatttttt ctttctccag tctcacaatt tttctagaaa taaaatcact
     3181 tctttgccgt ctatactctc
//
LOCUS       DAZKLM010000156         3103 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3764
            SAMEA115706913-rid22582563.denovo.163, whole genome shotgun
            sequence.
ACCESSION   DAZKLM010000156 DAZKLM010000000
VERSION     DAZKLM010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706913
            Sequence Read Archive: ERR13196647
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3103)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3103)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287823.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287823.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,004
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,849
            CDSs (with protein)               :: 2,849
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 41 of 96
            Pseudo Genes (incomplete)         :: 52 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 15 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3103
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706913-rid22582563.denovo.163"
                     /isolate="VRE3764"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            316..570
                     /locus_tag="ACG842_002701"
     CDS             316..570
                     /locus_tag="ACG842_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFQ0249177.1"
                     /translation="MGKELKVRKIGNSVGVILPSSLGLKSGDIIQAKQEGNLFILDTT
                     QIAKEHDRKLIEESFQDFEKGLTVSEIEMVKAFGKYGWSE"
     gene            571..888
                     /locus_tag="ACG842_002702"
     CDS             571..888
                     /locus_tag="ACG842_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFQ0249178.1"
                     /translation="MKEYTLVYSKSFQTSLETIIEEWENELALSPASIKRFVGAIQKA
                     LELTKNFPTMHEEVSEIYGMDIPTYRIVIGKSYALFYRIDHEKKEILVGRIFQSKQMK
                     LGF"
     gene            1447..2382
                     /locus_tag="ACG842_002703"
     CDS             1447..2382
                     /locus_tag="ACG842_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0249179.1"
                     /translation="MNILNEKRYVWFAGKKYITTFKRLSELMQQTVKSAMPEGYKFNF
                     VQSSVSTESIYFSILSESRLFHFRISSHVKKTDVDIYTFRTNNYKNLNDLSRSISEYL
                     KGNSYLKIRFSHILILLPYLNENFTLIQVDKSFIASGNQGNMNITSNKTSRVLLALMG
                     YGILYRDDQNGQILINRAGRSLLMKYRKFIPEDFQNLQLENMRLEDLFRLAEDDKYRF
                     EVLKIQKNELLNKLYPENAIKAHFGDANHMYSWFIPKRFRGKINAGDQVKVLNRNSTD
                     VVMITDIYVEDEDIIKYMKPVIKLVNKNKGTRGAS"
     gene            2476..3103
                     /locus_tag="ACG842_002704"
                     /pseudo
     CDS             2476..3103
                     /locus_tag="ACG842_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS982-like element ISEfm1 family transposase"
BASE COUNT         1120 a          464 c          571 g          948 t
ORIGIN      
        1 acaaaataaa aaggcaatac gccttagttg tcggtcttcg agttaccaac ggtaaccctc
       61 ataccgaacg gcattcatca cccacctata gaggatgggc gacttctgcc taattctgtt
      121 aaatggatgt taacggaagt gtatggtgag tagttcactc gtattttgat atcgtttgta
      181 tatgttttca gcgttctaag tggcttattt tgtccaataa taatatggag tggaatccct
      241 taagaaacac cgttggcaat tgttgttata acatgttata atttattcat caaaaactaa
      301 aaggagtgtg ataacatggg aaaagaacta aaggttagaa agatcggcaa tagcgtggga
      361 gtgatccttc caagctctct tggattaaaa agcggagaca ttatccaagc caaacaagaa
      421 ggcaacttat tcatattaga tactactcaa atagcaaaag aacacgatcg caaattgatt
      481 gaagaaagtt tccaagactt tgagaaaggt ttaacagttt cagaaattga aatggtaaaa
      541 gctttcggaa aatacggctg gagtgaatag atgaaagagt acacgctagt ttattctaaa
      601 agttttcaga ccagccttga aacgataata gaagaatggg aaaatgagct tgctctttct
      661 ccagccagca taaaaagatt tgtaggtgcg atccagaagg ctttagaact aaccaaaaat
      721 tttccaacta tgcatgagga agtttccgaa atctatggaa tggatatccc aacatatcgg
      781 atcgtgatcg gcaaaagcta tgccttattt tatcggatcg accatgaaaa aaaagagata
      841 cttgtaggac gtattttcca atctaaacaa atgaaactag gcttttaaca ataattagga
      901 aaaagtcgct catcctctac aggtgggtga ttaatgccgc tcgatatggt tgttaaccgt
      961 cggttactcg aagattgata tataagactt agtacctttt tattttgctc catgttatgg
     1021 ccgaaggtct aaagatacaa tcattagtct agagaaaaat taagaaccgt aggaactacg
     1081 gggatcgctt ggtgaataag agacactgct attagtgaaa aaataagata tcaagtacgc
     1141 tctattccca aaaatttcgt gactttactc atgagaggtt caagtagtat atttttaatc
     1201 aaaattgacg taacttttaa taaatactac ctaaataagg tagtatttat ttttgtgttt
     1261 taaaagagga agtccaatat atctatggga aacttcttaa atattatgtt ggtacatgaa
     1321 aatatacatc cctgctagac tatttacaac attttacttt gtacgtattc ttgttttaaa
     1381 aggaatggga taaaatatag gtacaataac tatttgatgc taaatataat taatggagga
     1441 gagatagtga acatacttaa tgaaaaaaga tatgtatggt ttgctggtaa aaaatatatt
     1501 actactttca aaagattatc cgagttaatg cagcagactg ttaaaagtgc tatgcctgaa
     1561 ggatacaagt ttaattttgt tcaatcttca gtatctactg aaagtatcta tttttcaatt
     1621 ctttctgaga gtaggctttt tcattttaga ataagttctc atgtaaagaa aactgatgtt
     1681 gatatatata cgttccgtac aaataattat aaaaatttaa atgatttgag tagaagtatc
     1741 agtgaatatc tcaaggggaa tagctatctc aagattcgat tttcacatat attaatttta
     1801 ctaccatatt tgaatgagaa tttcacccta attcaagtag ataagtcttt cattgcctca
     1861 ggtaatcaag gaaatatgaa tattacaagt aacaaaacgt cacgtgtgct attggcgttg
     1921 atgggttacg gaattcttta tagagatgac caaaatggcc agattctaat aaatcgggct
     1981 gggcgttcat tattaatgaa atatagaaaa tttataccgg aagattttca aaacttacag
     2041 ttagaaaaca tgcgtttaga agatttattt agattagcag aagatgataa gtatagattt
     2101 gaagttttga aaattcaaaa gaatgaacta ttgaataagt tatatcctga gaatgcgata
     2161 aaagcacatt ttggtgatgc taatcatatg tatagttggt tcattcctaa aagatttaga
     2221 gggaaaatca atgctggaga tcaagtgaaa gtactcaatc gaaatagtac agatgtcgta
     2281 atgataacag atatctatgt agaagatgaa gatataatca aatatatgaa gcctgtgata
     2341 aaacttgtta ataaaaataa aggaacccgt ggtgctagtt aattttttcc tagagaaaaa
     2401 tgtctaaaca tgttggtaaa tcaacaaaaa aacgcataga gtagtctcaa aactcccaag
     2461 aaatgaggac tacatatgcg ttcatcaaaa tatactgaac attatacaga tacttttata
     2521 acattcaagg aaattatgag aactgtttct aacatatacc acaattgtgt accagataag
     2581 attaaaaacc gaagaaatac tgatcaactg aagtaacgcg atacagtgat tattgcttgt
     2641 gttatatggg gcataattaa tggctatact agccaaagag ccacgtatag agcggtttgt
     2701 tccgtcttat ttcctaatgg tgactttcct agtaggagtc gattcactcg cttaagttca
     2761 aacttagctt acactatcaa gattattcga tactttttca tcaagaagct caccaaaggt
     2821 gaactagtcg gaattataga tagttttcct agtccattat gtaaaccagt tagaaataga
     2881 caagcaaaac tattgaatca aatagccaaa gttggctata actcaacaaa aaaatcttat
     2941 ttttatggtc ttaaaattca tatgatcgtt actaagactg gctttccgat tacttactca
     3001 atcacgaatc caggtgtcca tgacgtgaaa gtgttagaga ctttatcgga agaagcgaat
     3061 cttcccaata tcctagggga caaaggatat attagccata aaa
//
LOCUS       DAZKLM010000157         3044 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3764
            SAMEA115706913-rid22582563.denovo.164, whole genome shotgun
            sequence.
ACCESSION   DAZKLM010000157 DAZKLM010000000
VERSION     DAZKLM010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706913
            Sequence Read Archive: ERR13196647
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3044)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3044)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287823.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287823.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,004
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,849
            CDSs (with protein)               :: 2,849
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 41 of 96
            Pseudo Genes (incomplete)         :: 52 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 15 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3044
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706913-rid22582563.denovo.164"
                     /isolate="VRE3764"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(24..203)
                     /locus_tag="ACG842_002705"
     CDS             complement(24..203)
                     /locus_tag="ACG842_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0249180.1"
                     /translation="MGKFNRALVFSAPLIIYALGLWGSRQALIGTIVYMVWIFMGLDE
                     AEAEYRAKKPTEGAD"
     gene            complement(190..528)
                     /locus_tag="ACG842_002706"
     CDS             complement(190..528)
                     /locus_tag="ACG842_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF771 domain-containing protein"
                     /protein_id="HFQ0249181.1"
                     /translation="MQYLEAKIPIPEGYVIISQVDYEELKKADDTGRWMTLPEVLERI
                     NRKYDWFTSRVLKNPRYRNIIDIEKNKNGFVYYPVEGRDTYLFLRSKTLEFLETNFSE
                     ILRRQADGKI"
     gene            complement(544..747)
                     /locus_tag="ACG842_002707"
     CDS             complement(544..747)
                     /locus_tag="ACG842_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0249182.1"
                     /translation="MTDIAEITQRDREKIKEYVESSKFLTYTMLAERFGISKSYLSLI
                     LNGKKTSAEANRIIDSIITMYEL"
     gene            complement(762..1538)
                     /locus_tag="ACG842_002708"
     CDS             complement(762..1538)
                     /locus_tag="ACG842_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor KilAC domain-containing
                     protein"
                     /protein_id="HFQ0249183.1"
                     /translation="MNTPQIFNFEQNEVRTILVNDEPYFVGKDVASVLGYSNTKDALS
                     RHVDLEDKMGSRITTSGQSREMTIINESGLYSLILKSKLPSAKKFKRWVTSEVLPAIR
                     KHGGYLTPEKVEEALLNPDTIIQLATQLKEERTGRLIAEQKIAEYEPKISYLDSILSS
                     TDSVTISQIAADYGMSPQQMNKLLHKLGIQKKVGNQWLLCKKHMNQGYTKSHTTEIPK
                     ADGGTKIVMNTKWTQKGRLFIYELLKKEGYYPQMDLEEIG"
     gene            1837..2157
                     /locus_tag="ACG842_002709"
     CDS             1837..2157
                     /locus_tag="ACG842_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFQ0249184.1"
                     /translation="MNTYEIIKDLAKRKKISIRQLEINFGYSNGYLAKWKNNTPNSDE
                     LSRIADYFDVSVDYLLGREERETPKHVDLSEDDTVFSFDGKEISKETMRKAIAIAKAL
                     EENE"
     gene            2175..2606
                     /locus_tag="ACG842_002710"
     CDS             2175..2606
                     /locus_tag="ACG842_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HFQ0249185.1"
                     /translation="MYLKLKEMLSEYNLKVVYMEMKEPGFYYPKPRIIFLNENLYGET
                     AEAFHLSHELAHFSASHFEFSVLYDTSTTFHSKFETEADKIAILILLNIYIENELTDE
                     SQFNLEKFMEYYSIQNKLRYTCYAVCQCYFKKKYSYARQYV"
     gene            2736..>3044
                     /locus_tag="ACG842_002711"
     CDS             2736..>3044
                     /locus_tag="ACG842_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4429 domain-containing protein"
                     /protein_id="HFQ0249186.1"
                     /translation="MEKEILIKSPGKTLIKVTDDSISIIRKGFINLVNQGIKGEKTIP
                     FKNISAVQLKKPGMSNGYIQFTLLGGNESRGGILAATKDENTVMFTKKYWNEMENLKK
                     Y"
BASE COUNT          964 a          521 c          478 g         1081 t
ORIGIN      
        1 tagtttctct tttctattca tttttagtca gccccctcgg ttggcttttt cgctctgtac
       61 tcagcttcag cttcatcaag ccccataaaa atccaaacca tgtaaacaat cgttcctatc
      121 aacgcttgcc tgcttcccca aagtcctaaa gcgtagatga ttagcggtgc gctgaacact
      181 agcgctctat taaattttcc catccgcttg cctccttaag atttccgaaa aatttgtttc
      241 taaaaattca agtgttttac ttcttaaaaa tagatatgtg tctcttcctt caactggata
      301 atagacaaat ccatttttgt ttttctcgat atcgataata tttctatatc ttgggttctt
      361 taaaactcta gaagtaaacc aatcatattt tctgttaatc cgttctagca cttctggcaa
      421 cgtcatccat ctaccagtat catcagcttt ttttaactcc tcataatcca cttgggagat
      481 aattacatag ccttctggaa ttgggatttt tgcttctaga tattgcatta gctgttcctc
      541 cctttacaat tcgtacatag tgataatcga atctataatt ctgtttgctt ctgcagaagt
      601 ctttttaccg tttaaaatta aagataagta gcttttgcta attccaaatc tttcagcaag
      661 catggtgtaa gttaagaact ttgaactttc gacatattct ttgatttttt ctctatctcg
      721 ttgagtgatt tctgcaatgt cagtcatact aaaactcctt tctaaccaat ttcctctaaa
      781 tccatttgag ggtaatatcc ttcttttttt agtaattcgt agataaatag acgccctttc
      841 tgtgtccatt tggtattcat tacaatttta gtgccaccat cggctttcgg gatctcagtt
      901 gtatgagatt ttgtgtatcc ttggttcatg tgttttttgc acaataacca ttggttaccg
      961 acttttttct gaatacctag tttatgaagt aatttattca tctgttgtgg agacatccca
     1021 taatctgctg caatctgact aattgttact gaatctgtag aagataatat gctatctaaa
     1081 taggagattt taggttcgta ctctgcaatc ttttgttctg cgattaatct tccagttctt
     1141 tcttctttta gttgagttgc taattgaatg attgtatctg gattaagcaa agcttcttct
     1201 actttttctg gagttagata acctccatgt tttctaattg ctggcaacac ttcacttgtt
     1261 acccaacgtt taaatttttt ggcagaggga agttttgatt ttaagattaa actgtacaaa
     1321 ccagattcgt tgatgattgt catctctctt gattgacctg aggtcgtgat tcgcgacccc
     1381 atcttatctt ccaaatctac atgccgcgac aaagcatctt tagtgtttga ataacccaaa
     1441 acactcgcaa cgtctttgcc tacaaaatat ggttcatcat ttactaaaat tgtccgaact
     1501 tcgttttgtt cgaaattaaa aatttgcggt gtgttcatat ttctcattcc tttcttttat
     1561 attcgtaaac aaatttaaca acttttttct aaattcaatt gacagtatct gagtaatact
     1621 ctatactaag agcataatta aataagacat aaaaacattg attttaaagc tttcttggcg
     1681 gtctgcattt acatatcaat agtgtttttt gttgtctttt tagttgttaa atctgtttac
     1741 aagatatagt atagctcatt actcagaatt agtcaaatta tttctgcgta tttttctaaa
     1801 ctttttttgt aaacatttag aaaggttgtt ttatcgatga atacctatga aataataaaa
     1861 gacttggcga agaggaaaaa gatatctata cggcagttag aaattaattt tggatattct
     1921 aacggttatc tagcaaagtg gaaaaataat actcctaatt cagatgaatt aagtcggatt
     1981 gctgactatt tcgatgtctc tgtagactac cttctaggta gagaagaaag agaaacccct
     2041 aaacatgtgg atttatcaga agacgatact gttttttctt ttgatggaaa agaaatatct
     2101 aaggagacaa tgcgtaaagc gattgcaatt gctaaagctt tagaggaaaa tgaatagttg
     2161 gagtgatggt ttgtatgtat ttgaaattga aagaaatgtt gagcgaatac aatttgaaag
     2221 tagtttatat ggagatgaaa gagcctggtt tctactatcc aaaacccaga atcatttttt
     2281 tgaatgaaaa tctatacggt gaaactgcag aagcctttca tttatctcat gaacttgcac
     2341 atttcagcgc ctctcatttt gaattttcag ttttgtacga tacttctaca acttttcact
     2401 caaaatttga aacagaagca gataaaatag ctattctaat tttattaaat atctatattg
     2461 agaacgaact gactgatgaa tctcagttta atctcgaaaa atttatggag tattattcta
     2521 ttcagaataa gctcagatac acttgttatg ctgtctgcca atgctatttt aagaaaaaat
     2581 attcttacgc aaggcaatat gtatgaatac atcaagaatt attaaaaaaa ccgatttcag
     2641 caaaacatta aaaaagcccg tgctgcaaca cgggctcttt cctcatatat gagcttctac
     2701 aaaaaaaatc atatcacaga aatgaggaag aaaaaatgga aaaagagatt ttaataaaat
     2761 ctccaggaaa aaccttgatt aaagtaactg atgattctat ttcaattatt agaaaaggat
     2821 ttataaattt agttaatcaa ggtattaaag gagaaaaaac tatccccttt aaaaatatat
     2881 cagccgtaca attaaaaaaa ccaggtatga gtaatggtta tatacaattt accttgctag
     2941 gcggaaacga aagtcgcgga ggtatattag cggccacgaa agatgaaaac accgttatgt
     3001 ttacaaaaaa atattggaat gaaatggaaa atttaaaaaa atat
//
LOCUS       DAZKLM010000158         2932 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3764
            SAMEA115706913-rid22582563.denovo.165, whole genome shotgun
            sequence.
ACCESSION   DAZKLM010000158 DAZKLM010000000
VERSION     DAZKLM010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706913
            Sequence Read Archive: ERR13196647
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2932)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2932)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287823.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287823.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,004
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,849
            CDSs (with protein)               :: 2,849
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 41 of 96
            Pseudo Genes (incomplete)         :: 52 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 15 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2932
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706913-rid22582563.denovo.165"
                     /isolate="VRE3764"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            88..984
                     /locus_tag="ACG842_002712"
     CDS             88..984
                     /locus_tag="ACG842_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HFQ0249187.1"
                     /translation="MIQYYYTKKEWGVVMEKEKLKILEELRRILNNKNEAIIILNNYF
                     KGGVGKSKLSTMFAYLTDKLNLKVLMIDKDLQATLTKDLAKTFEVELPRVNFYEGLKN
                     GNLASSIVHLTDNLDLIPGTFDLMLLPKLTRSWTFENESRLLATLLAPLKSDYDLIII
                     DTVPTPSVYTNNAIVASDYVMIPLQAEEESTNNIQNYISYLIDLQEQFNPGLDMIGFV
                     PYLVDTDSATIKSNLEELYKQHKEDNLVFQNIIKRSNKVSTWSKNGITEHKGYDKKVL
                     SMYENVFFEMLERIIQLENEKE"
     gene            1076..1291
                     /locus_tag="ACG842_002713"
     CDS             1076..1291
                     /locus_tag="ACG842_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002401482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
                     /protein_id="HFQ0249188.1"
                     /translation="MIVGNLGAQKAKRNDTPISAKKDIMGDKTVRVRADLHHIIKIET
                     AKNGGNVKEVMDQALEEYIRKYLPDKL"
     gene            1309..1581
                     /locus_tag="ACG842_002714"
     CDS             1309..1581
                     /locus_tag="ACG842_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HFQ0249189.1"
                     /translation="MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELDTKNTRLLEV
                     NLLNQLEVAQEVDLFQQPFEELQAIHEYWRSMNQYSKQILTKEKVA"
     gene            1583..2446
                     /locus_tag="ACG842_002715"
     CDS             1583..2446
                     /locus_tag="ACG842_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HFQ0249190.1"
                     /translation="MANITDFTEKQFEDRLEKNVERLTKNRLAVESPTAFLLGGQPGS
                     GKTSLRSAISEETQGNVVIIDNDTFKQQHPNFDELVKLYEKDVVKYVTPYSNRMTEAI
                     ISRLRDKGYNLVIEGTGRTTDVPIQTATMLQAKDYETKMYVMAVPKINSYLGTIERYE
                     TMYADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSSLETP
                     SISPKETLERELNRKVSGKEIQPTLERIEQKMVQNQHQETPEFKAIQQKMESLQPPTP
                     PIPKTPKLPGI"
BASE COUNT         1163 a          464 c          510 g          795 t
ORIGIN      
        1 aaagagggct tttatatatt aatcacaaat cacttatcac aaatcacaag tgattaatca
       61 caaatcactt gtgatttgtg attcttaatg atacaatatt actatacaaa aaaagaatgg
      121 ggcgtagtta tggagaagga aaaactaaaa atacttgaag aattaagacg tattttaaac
      181 aataaaaatg aagcaattat tatcttgaat aattacttta aaggtggtgt tggaaagtcc
      241 aaattatcga ctatgtttgc ttacttgaca gacaaattga atttaaaagt tttaatgatc
      301 gataaggact tacaggcaac attgacaaaa gacttagcaa aaacttttga ggtagaattg
      361 ccacgtgtca atttttatga aggcttgaaa aatggaaact tggcttcttc tattgttcat
      421 ttgactgata atttagactt gatccctggc acgtttgatt tgatgttact gccaaaatta
      481 actcgctcat ggacttttga aaatgaaagt agattgcttg ctactctttt agcaccttta
      541 aaaagtgact atgatctcat tattattgat actgtaccaa cgccaagcgt ttatacaaat
      601 aatgcaatcg tggcgagtga ttacgttatg atccctttac aagcagaaga agaaagtaca
      661 aacaacattc aaaactatat ttcctatttg attgatttac aagaacaatt taaccctgga
      721 ctagatatga tcggttttgt tccttattta gttgatacgg acagcgcaac gataaaatca
      781 aacctggaag aactgtacaa gcaacataaa gaagataact tggttttcca aaatattatc
      841 aagcgaagta ataaagtaag tacctggtct aaaaatggaa ttacagaaca caaaggctat
      901 gacaaaaaag ttttatccat gtatgagaac gtattttttg aaatgcttga gcgaattatt
      961 caattagaaa acgaaaaaga atagaatcac aaatcacaag tgattaatca caaatcactt
     1021 gtgatttgtg attgttgatg ataaaataag aataagaaga aatagaaaga agtgagtgat
     1081 tgtgggaaat ttaggcgcac aaaaagcaaa acgaaatgat acaccaatca gtgcaaaaaa
     1141 agatataatg ggagataaga cggttcgtgt tcgtgctgac ttacaccata ttataaaaat
     1201 tgaaacagca aaaaatggcg gaaacgtaaa agaagttatg gatcaagcct tagaagaata
     1261 tatacggaaa tatttacctg ataaacttta aaaaggagtg aaaatgaaat ggcagttacg
     1321 tatgaaaaaa catttgaaat agagatcatt aacgaattat cggcaagcgt ttataatcga
     1381 gtattaaact atgttttgaa ccatgaacta gatactaaaa atactcgttt actagaagtg
     1441 aatcttttaa atcaattaga agtggcacaa gaagttgatt tatttcaaca accatttgaa
     1501 gaattacaag ctattcatga gtattggcgg tcaatgaatc aatattcaaa acaaattttg
     1561 acaaaagaga aagtggctta acatggcgaa tattactgac ttcaccgaaa agcaatttga
     1621 agatcgttta gaaaagaatg ttgaacgact aactaaaaat agactagcgg ttgaatcgcc
     1681 aaccgctttt ttacttggtg ggcaaccagg atcaggtaaa actagtttgc gatcggcaat
     1741 ttccgaagag acacaaggaa atgttgttat cattgataat gatacgttca aacaacagca
     1801 ccctaatttt gatgaactag tgaaacttta tgaaaaagac gtagtaaaat acgttacccc
     1861 ttattctaat cgcatgacag aagcgatcat aagccgtttg agagataaag ggtataattt
     1921 agtgattgaa ggtacaggac gaacaacaga cgttcctatt caaaccgcaa caatgcttca
     1981 agccaaagat tatgaaacaa aaatgtatgt catggcagta cctaaaatca actcatattt
     2041 aggaacaatt gaacggtatg aaactatgta tgcagatgat ccaatgacag ccagggcaac
     2101 accaaaacaa gcgcatgata ttgttgtcaa aaacttaccg accaatttag aaacccttca
     2161 taaaacgggc ttatttagcg atataaggct ttacaataga gaaggagtga aactctattc
     2221 aagcttagaa acgccttcca ttagtccgaa agagacctta gaaagagaat taaatcgtaa
     2281 agtatcaggg aaagaaattc aaccgacttt ggagagaata gagcaaaaaa tggttcaaaa
     2341 tcaacaccaa gaaacccctg aatttaaagc aattcaacaa aaaatggaaa gcttgcagcc
     2401 acctacacca ccaataccca aaacacctaa acttccaggg atttaaacct taaaaaagta
     2461 aaagagtagt taccaaaaaa cggtaactac tcttttttta taaaaacatt tcctcatttt
     2521 ttaaaagctg atcgagaaat aaagcacaca actgtattct ttcttttttc agttggtaca
     2581 ttttgcagtc tgtaacgtgc catttactgt ttgttacctt aacatatcca gtcgaaaaca
     2641 caagggtata taaacgagca agctcgccct tgtgttttct ttgtgtctat gttgcgttct
     2701 cgtcacagca aacgacacga acacactggc gaaaatgaac caacaaaacg aaaaaagaaa
     2761 ggagcgtaca ctgtaattta tttctagctt ttaaaacatg aggaaaacag cttcacttcc
     2821 tcaaacgcct aacaaaaatg gttgtcaaaa aactaaaaat caaaaaaaga aaagaggaaa
     2881 agaaaatgga aaaaacaaaa aaattacaat tagaagattt tacagaaaat gg
//
LOCUS       DAZKLM010000159         2905 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3764
            SAMEA115706913-rid22582563.denovo.166, whole genome shotgun
            sequence.
ACCESSION   DAZKLM010000159 DAZKLM010000000
VERSION     DAZKLM010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706913
            Sequence Read Archive: ERR13196647
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2905)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2905)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287823.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287823.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,004
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,849
            CDSs (with protein)               :: 2,849
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 41 of 96
            Pseudo Genes (incomplete)         :: 52 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 15 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2905
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706913-rid22582563.denovo.166"
                     /isolate="VRE3764"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            122..937
                     /locus_tag="ACG842_002716"
     CDS             122..937
                     /locus_tag="ACG842_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0249191.1"
                     /translation="MVKIAGLSKGGKTVSQLYKQNYRDWRQFRKNIKDSYFILPTELE
                     KYLPNNKAINLYLYYCFKAKNEGGDSWHSVNTIAESLGVTTKTINTWNKTLVDLGMIA
                     RIDDNHLSKSTILLPLSSYYKLALDSSLKDVTESTVHEIDGNLVAVYHLFEWRKNEAD
                     ENYNVPYNTLCCVFKRKTQKDTIYKFILVQDEKISNFELSTPSSKIYDDAYRFECQDL
                     EALLSANNLNNIEHENFVVNTRFNLVSEKDSDLLDLLIELTKNLDKKENITVL"
     gene            1063..1320
                     /locus_tag="ACG842_002717"
     CDS             1063..1320
                     /locus_tag="ACG842_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0249192.1"
                     /translation="MANLYIENKGDLNAIITLIVVMVKSIGNITIGMLFIILAAVLIF
                     MIVIWFLNERSKDNQHRRDMEIKSIESIDYLSIITLKKPRS"
     gene            complement(1572..2144)
                     /locus_tag="ACG842_002718"
     CDS             complement(1572..2144)
                     /locus_tag="ACG842_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFQ0249193.1"
                     /translation="MTKYGYARVSTASQSTKEQIQQLLENGVEKKNIFSEKFTGTKRD
                     RPKFNQLLAGLKPGDEIVVTKLDRFARNTREALEIIEPLLDDNVTIKVLNLGTIENTS
                     MGRMVTRTLLSVAEMERDMIVERTQEGKMFARKNNPNFREGRPKATITPKKRHAYELL
                     TSGKSYKEVEAITGFSRSTLFRIKKKIEES"
     gene            complement(2160..2765)
                     /gene="fic"
                     /locus_tag="ACG842_002719"
     CDS             complement(2160..2765)
                     /gene="fic"
                     /locus_tag="ACG842_002719"
                     /EC_number="2.7.7.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0070733 - AMPylase activity [Evidence
                     IEA];
                     GO_process: GO:0018117 - protein adenylylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein adenylyltransferase Fic"
                     /protein_id="HFQ0249194.1"
                     /translation="MILENKLGLTNQVELAKVEEKLSKQKAKELYDCGKINEIEVGTF
                     KGLSEIHEFLFSDIYDFAGKIRSVNIAKGNFRFAPVMYLEHSLQHIDQMPQTSFEEII
                     KKYVEMNIAHPFREGNGRSTRIWLDLILKEELQKVVDWNLIDKADYLSAMERSPINDL
                     EIRYLISNALTDKIDDRELYMKGIDVSYFYEGYSEYTIDDL"
BASE COUNT          950 a          528 c          450 g          977 t
ORIGIN      
        1 taaataaaaa ataattacta tctagtacga aattaattac ataggataat atattaaggt
       61 gctggactac aagtaaaaaa tactgtatga tcatgacaga ttcaagaaag gagcaataag
      121 attggttaag atagcaggac tttctaaagg tggaaaaacc gtttcacaac tttataaaca
      181 aaactacaga gactggagac agttcagaaa aaacattaag gattcttact ttattctacc
      241 aacagaactt gaaaaatatt taccgaataa caaagctatt aatttatatc tgtattactg
      301 ttttaaggca aagaatgaag gcggggattc ctggcattcg gtcaatacca tagcagagtc
      361 tctaggagtg acaactaaaa ctataaacac ctggaataaa accttagttg atttaggtat
      421 gattgcacgt atagacgata accatctcag taaatcgaca attttacttc ctctctctag
      481 ttactataaa ttagcccttg atagttcttt aaaggatgtt accgagagca ctgtccatga
      541 aattgatggc aacttggttg cagtttatca tttgtttgaa tggagaaaaa atgaggctga
      601 tgaaaattat aacgttcctt ataacacact ctgttgtgtt tttaaaagaa aaacacaaaa
      661 agatactatc tataaattta tcttggtcca agatgagaaa ataagtaatt tcgaattaag
      721 cactccttct agtaagattt atgacgatgc atatagattt gaatgtcaag atctagaagc
      781 cctcctttcc gcaaataatc taaacaatat agaacatgaa aactttgttg taaacactag
      841 atttaattta gtttctgaaa aggactcaga tttgttagat ttgctgatag agcttactaa
      901 aaatctagat aagaaagaaa acattactgt actataacta gcatcggtcc atcattaagt
      961 ttggactatt agaacacttg ctattacaga aaaaggtatg ctagccacgc ttactattta
     1021 ttggtatcaa acaagtatct taatttgaaa ggaagatgat ttatggctaa tttatatata
     1081 gagaacaaag gagacttgaa tgccattatt actttaatcg tagttatggt aaaatcaata
     1141 ggtaatatca caataggaat gctgtttatt atattagcag cagtgctgat ttttatgata
     1201 gttatttggt tcttaaatga aagatctaaa gacaatcaac accgtaggga tatggagata
     1261 aaatcaatag agtcaataga ttatttatcg ataataacac taaaaaagcc acgttcctaa
     1321 acgtgactta cgctgaacga tatgtgttgt ttggtagacg aacaaatcgt tcattataaa
     1381 tatatgatgg tatcctaata tagtaagcgt ggctagcata cctttaatta ctttaccttt
     1441 tacagttacc atacacattt tttgagcaga aatcaagtaa ataaaaaaat attatatttc
     1501 acaacatgct aatagactca tatctagaac gcattaagta tttacctgtc tggttcgctg
     1561 agacttcttt tttaactttc ttcaattttc ttctttattc taaaaagcgt actgcgactg
     1621 aaaccggtta tagcttcaac ttccttatac gatttacctg aagtaagcag ttcatatgcg
     1681 tgcctcttct tcggtgtgat tgttgctttc ggacgtcctt ctctgaaatt aggattattc
     1741 tttcttgcaa acatttttcc ttcttgggta cgttccacaa tcatatcacg ttccatttct
     1801 gctacactca acaacgtccg tgtcaccatt cgccccatgg acgtattttc aatcgttcct
     1861 agattcaaaa ccttgatcgt tacgttatca tcgagtaaag gttcaatgat ctctaatgct
     1921 tctctcgtgt tacgagcaaa tcgatcaagc ttcgtaacaa ctatttcatc tccaggcttc
     1981 agaccagcca acagctgatt gaattttggc cgatcccgtt ttgtacccgt aaatttttct
     2041 gaaaaaatat tcttcttctc tacgccgttc tcaagcaact gttgaatttg ttcttttgta
     2101 ctttgagaag cggttgagac acgtgcatat ccatatttcg tcatttggtc cacctacttt
     2161 tacaaatcat ctatcgtata ctcagaatac ccttcataga aatagctgac atcgattcct
     2221 ttcatatata actcgcgatc gtcaatttta tcagtcagag cattggaaat tagatagcgt
     2281 atttccaaat cgtttatggg acttcgctcc attgcagaaa gatagtctgc tttatcgatt
     2341 aagttccaat ctacaacttt ttgcaattct tcctttaaaa tgagatccag ccaaattcta
     2401 gtacttcggc catttccttc tcgaaatggg tgagcgatat tcatctccac atatttcttg
     2461 ataatctctt caaaagaagt ttgtggcatc tgatcaatat gctgtaacga atgttctaaa
     2521 tacataactg gtgcaaatcg aaaatttcct tttgcaatat tgaccgatcg gatttttccg
     2581 gcaaaatcgt aaatatccga aaatagaaat tcatgaattt cagataaacc tttaaatgta
     2641 ccaacttcga tttcatttat ttttccacaa tcgtacaatt ctttcgcttt ttgtttactg
     2701 agtttttctt caaccttagc caactcaact tgattggtta atcccaattt attctctaaa
     2761 atcatgcgaa atctccttaa ctttatgaca taagtttatg aaacactctc aaccccatta
     2821 tatcgcgatt ttgtttatgt gtcaaaaact attaagtttc tgacccccga tagatttttc
     2881 aagtgtctct attttgcaca gtcta
//
LOCUS       DAZKLM010000160         2825 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3764
            SAMEA115706913-rid22582563.denovo.167, whole genome shotgun
            sequence.
ACCESSION   DAZKLM010000160 DAZKLM010000000
VERSION     DAZKLM010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706913
            Sequence Read Archive: ERR13196647
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2825)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2825)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287823.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287823.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,004
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,849
            CDSs (with protein)               :: 2,849
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 41 of 96
            Pseudo Genes (incomplete)         :: 52 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 15 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2825
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706913-rid22582563.denovo.167"
                     /isolate="VRE3764"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            35..202
                     /locus_tag="ACG842_002720"
     CDS             35..202
                     /locus_tag="ACG842_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich KTR domain-containing protein"
                     /protein_id="HFQ0249195.1"
                     /translation="MCPVCGNKTRLKIREDTELKKFPLYCPKCRQENLIEIKQFKVTV
                     ITEPDAKTQSR"
     gene            complement(262..615)
                     /locus_tag="ACG842_002721"
     CDS             complement(262..615)
                     /locus_tag="ACG842_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0249196.1"
                     /translation="MRKKEDKYDFRAFGLAIKEARLKRGLTREQVGALIEIDPRYLTN
                     IENKGQHPSIQVLYDLVSLLHVSVDEFFLPANNLVKSTRRLQIEKYMDSFTDKELSLM
                     ESLASGINEARNIED"
     gene            1119..1538
                     /locus_tag="ACG842_002722"
     CDS             1119..1538
                     /locus_tag="ACG842_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009588541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HFQ0249197.1"
                     /translation="MRTSSFEHIVRLQFNSLMLIVIKNKVKSRNRQLARHSKREVLFC
                     EIAETKQVERGTIDTCFYDCVSFKVLHFTIYVSDETLGTVLHRLSEKQRSAILLRYFQ
                     GMNDREISELYHVSRSAICSRRTRGLRKLKMLLSERK"
     gene            1564..1770
                     /locus_tag="ACG842_002723"
     CDS             1564..1770
                     /locus_tag="ACG842_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004613734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0249198.1"
                     /translation="MICKASSGDEMAVKEILKFYDGYISKLCLRPFYHSESGKIIMQV
                     DEELKGEIHTDMMKAILKFEIGVK"
     gene            complement(1998..2171)
                     /locus_tag="ACG842_002724"
     CDS             complement(1998..2171)
                     /locus_tag="ACG842_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0249199.1"
                     /translation="MEKKNQAQLSEGGIDYVLDIINEPMVLSYAMEQVERLLVCAGKQ
                     AMRLIRLGEKIRR"
     gene            2183..2812
                     /locus_tag="ACG842_002725"
     CDS             2183..2812
                     /locus_tag="ACG842_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0249200.1"
                     /translation="MGQLSDNQQLCQERINPLLELLERVFSYYGQALLTVHRQQIIIL
                     VNRISRASLLSLLDKIQTKFNKMYQLQLNFGIGSLCYTEQETPQSFLHAKQVCEWIAF
                     HQSVNEIRFFEDLDLGIVLPAIPSDQRTLYVKRILKSLTEEEVHLFKKTLACFSKNNG
                     SIKNCSEELFIHKNTLQYRLNKFHSLTGYTPRNYDDYHILKLAFLLVQT"
BASE COUNT          938 a          479 c          512 g          896 t
ORIGIN      
        1 ggaggagata gttattataa actacaagtg gatattgtgt cctgtatgtg gaaataaaac
       61 acgattaaag ataagggaag atactgaatt aaaaaaattc cccctctatt gtccgaaatg
      121 cagacaagaa aatttaattg aaataaagca gttcaaagta actgtgatta cagagccaga
      181 cgcaaagacg cagagccgat aaaatgagat taatacaatc tcattttatc ggctctttcc
      241 gttatgtatg gattctttta attagtcttc gatgtttctt gcttcgttga taccgctggc
      301 taaagattcc attaaggata gttctttgtc tgtaaagcta tccatgtatt tctctatctg
      361 taatcgtcgg gtgcttttta ccaagttatt agcaggtaag aaaaattcat caacggaaac
      421 atgaagtaac gatacaaggt cataaagaac ttgtatgctg gggtgttgcc ctttattttc
      481 aatattagtt aagtaccgtg ggtcaatttc aatcaatgct cccacttgtt cacgagttaa
      541 acctcgtttc aatcgagctt ctttaatggc taaaccaaag gctctaaaat catatttatc
      601 ttctttttta cgcatagtag accacctcta tacattttat tgttcctact gaattaaaaa
      661 caggtataga aaaacgtgtt atatggttta taggtttata tttaataaaa agcactacta
      721 aacgccaata aaaaaaaccg ttatatggta gtgctattta cgctgttaaa atattgtata
      781 ttacttccaa atggcggttt gttggaggtc aacgtcgcca tgaagtacat catatacaat
      841 aaatttcctt acattgggtt cttgtcaaaa aaagtcgtct atctgcaata gataagtacg
      901 tccaccaatg tggttttata aatcatatag atagaataac agaagcatgt aaacagagaa
      961 ataaatctgt ttatatgctt ttttggctat tcagaacttt tttacaaagt ttatttatca
     1021 gtaatgcaac aaatccccct ttcacattgg gactaagagt gaaaggagat aaacgagcaa
     1081 ggctcacttc ctttcctaga cagaaagggg gtgagattat gaggacatct tctttcgagc
     1141 atatcgtcag attacagttc aattctttaa tgctgattgt cattaagaac aaagtgaaaa
     1201 gtcgaaacag acagcttgca agacattcta aacgtgaagt attgttttgt gaaatagccg
     1261 aaacgaaaca agttgaacgt ggaacgattg atacctgttt ttatgattgt gtatcattta
     1321 aagtcttgca ctttacaatt tatgtatctg atgaaacact tggtacagtt ttacatagac
     1381 tatcagaaaa acagcgtagt gcgattttat tacgttattt tcaaggaatg aacgacagag
     1441 aaatttcaga actgtaccat gtatcacgtt ctgctatttg cagtagaaga actagaggac
     1501 taaggaagtt aaaaatgctt ttgagcgaaa ggaagtaaag attatggcta tccctctttt
     1561 gaattgattt gcaaagcgtc aagcggagat gaaatggcag ttaaagagat tttgaagttt
     1621 tatgacggtt atatttctaa attatgcttg cgtccttttt accactctga aagtggtaaa
     1681 atcattatgc aggttgatga agaactaaaa ggagaaattc atacagacat gatgaaagca
     1741 atattgaaat ttgaaatcgg agtgaagtaa tcaactgtat aaaaaaatcg ggagatacac
     1801 agtttcgtgt tatctccctt agtagtttta cggtcatatc aaggctcatt caatcgtggt
     1861 aaattcgtgg taagatgagt gtttttctat acttcaaaat gcttgaatgt atagtgttta
     1921 tgcggataat tgaaaattag atataaaatt taataaaaac caaaaatgca cggtcgccat
     1981 gaagggaccg tgtattttta tcttcttatt ttctcaccta atcgtatcag ccgcatcgct
     2041 tgttttcctg cacaaacaag caatcgctct acctgttcca tcgcataaga aagtaccatc
     2101 ggttcattga tgatatcaag tacatagtcg ataccaccct cacttaattg agcttgattt
     2161 ttcttctcca tattatgtag ttgtgggaca actttctgac aatcagcaat tgtgtcaaga
     2221 acggatcaat cctttattgg agttgctcga aagggtcttc tcttattacg gacaggcttt
     2281 gctcactgta catagacaac aaatcattat cctagtcaat cggatttcca gagcctcatt
     2341 gctatcattg cttgataaaa tccagacaaa gtttaataaa atgtatcagt tacagctaaa
     2401 ctttggaatt gggtcgcttt gttatacaga gcaagagaca ccgcaatctt tccttcatgc
     2461 caaacaagtt tgcgaatgga tagctttcca tcaatctgtg aacgagatcc gcttttttga
     2521 agatttagac ctcgggatcg ttttaccagc aatcccgtca gatcaacgaa cattatatgt
     2581 aaaacgtatt ttgaaaagcc tgacagaaga agaagtacat ttgttcaaga agactttggc
     2641 atgcttcagt aaaaataacg gaagcatcaa aaattgctca gaagaattat ttattcataa
     2701 aaacaccttg caatatcgat tgaacaagtt ccattctctc actggttata cccctagaaa
     2761 ctatgacgat tatcatattc taaagctggc ttttttgttg gtacaaacat gacaactaca
     2821 aaaaa
//
LOCUS       DAZKLM010000161         2783 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3764
            SAMEA115706913-rid22582563.denovo.168, whole genome shotgun
            sequence.
ACCESSION   DAZKLM010000161 DAZKLM010000000
VERSION     DAZKLM010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706913
            Sequence Read Archive: ERR13196647
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2783)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2783)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287823.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287823.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,004
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,849
            CDSs (with protein)               :: 2,849
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 41 of 96
            Pseudo Genes (incomplete)         :: 52 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 15 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2783
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706913-rid22582563.denovo.168"
                     /isolate="VRE3764"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            233..1558
                     /locus_tag="ACG842_002726"
     CDS             233..1558
                     /locus_tag="ACG842_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HFQ0249201.1"
                     /translation="MMDYSKEPVNDYFLIDMKSFYASVECIERNLDPLTTELVVMSRA
                     DNTGSGLILASSPAAKKKYGISNVSRPRDLPQPFPKTLHVVPPRMNLYIKRNMQVNNI
                     FRRYVADEDLLIYSIDESILKVTRSMNLFTTEGTRSQRRKKLAQMIQENIKEELGLLA
                     TVGVGDNPLLAKLALDNEAKHNDGFLAEWTYQNVPDKVWNIPEMTDLWGIGSRMKKRL
                     NQIGIMSIKDLANWNPYTIKSHLGVIGLQLYFHANGIDRTDIAIPPEPTKEKSYGNSQ
                     VLPRDYTRRNEIELVVKEMAEQVAIRIRQHNCKTGCVHLYIGTSILETRPGFSHQMKI
                     PITDNTKELQNYCLFLFDKYYEGQEVRHVGITYSKLVYTDSLQLDLFSDPQKQIDEEN
                     LDKIIDKIRQKYGFTSIVHASSMLEGARSITRSTLVGGHAGGAGGLKND"
     gene            1551..1901
                     /locus_tag="ACG842_002727"
     CDS             1551..1901
                     /locus_tag="ACG842_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0249202.1"
                     /translation="MIDYNDRGRLKWAGFYLSDHTEKIDADNEQRANQNLAKEQMTTE
                     EVAEVLNAAVIKNRSVCIQKEERNAEGYYPEDIVSKILGSDELGIYVGKNKVHYDEIR
                     HIEFYQDNKWGRLE"
     gene            1898..2068
                     /locus_tag="ACG842_002728"
     CDS             1898..2068
                     /locus_tag="ACG842_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0249203.1"
                     /translation="MNIIQQYELKYITFDQLSEEIWGYGQRLINEVGVERFSFYVEAA
                     AGYHKYEYYIGY"
BASE COUNT          979 a          440 c          539 g          825 t
ORIGIN      
        1 ttttttctaa tataactaag caaaactgga taatacgaca cttattttca aattttcaca
       61 agactaatag taaatatcca tttaataaag tgatacaatg tgttgatttt acaaatcaac
      121 agaagaagta gggaaataac ttacctatgc ttcttttttt gtacatttaa tcgattcttc
      181 tttacaaaaa cgaacgtatg tttgtataat gtggaagaat ggagtgttat ttatgatgga
      241 ttattcaaaa gagccagtaa atgattactt tttgatcgat atgaaaagct tttacgcaag
      301 tgtcgaatgt atagaaagga atttagatcc attaacgact gaattagtcg tcatgagtcg
      361 agcggataat acaggatcag ggttgatttt agcttcttca ccagctgcaa agaagaagta
      421 cggtatttct aatgtcagtc gaccacgaga tctaccgcaa ccttttccta aaacgttaca
      481 tgtcgttcca ccacgcatga atctctatat taagcgaaat atgcaggtaa acaatatttt
      541 cagaaggtat gtagcagacg aagatttatt gatttactcg atcgatgaat cgatcttaaa
      601 agtgaccaga tcaatgaatc tttttacgac tgaaggaaca agaagtcaac ggagaaagaa
      661 gctggctcaa atgatccagg agaatattaa agaagagcta ggattgcttg ctacagtagg
      721 tgtcggagat aatcccttat tagctaaact tgctttagac aatgaagcaa aacataatga
      781 tggattcctt gctgaatgga cgtatcaaaa tgttcctgat aaagtatgga atattcctga
      841 aatgacagac ctttggggta ttggatctcg aatgaaaaaa agactcaacc aaattggtat
      901 catgagtatt aaagacttag ctaattggaa tccatacact attaagagcc atttaggtgt
      961 gattggctta cagctgtatt ttcatgcgaa cgggatcgat cgaacagata tagcgatccc
     1021 accagaaccg acaaaggaaa aatcatatgg taattcacaa gtgttaccac gagattatac
     1081 caggagaaat gaaatcgaac tggtggtaaa agaaatggcg gaacaagtag ctattcgtat
     1141 caggcaacat aactgtaaaa caggttgtgt ccatttatac attggcacgt cgatcttaga
     1201 aacgagacca ggtttttcac accagatgaa gatccctata acagataata caaaggaatt
     1261 gcagaattac tgtcttttct tgtttgataa gtattatgaa ggacaagaag taaggcacgt
     1321 tggaataacc tatagcaagc ttgtgtacac tgattcgctt cagctggatt tatttagtga
     1381 tccacaaaag caaattgacg aagaaaattt agataaaatt attgacaaga ttcggcaaaa
     1441 atatggtttt acgtcgattg tacatgcttc ttctatgtta gaaggcgctc gtagtattac
     1501 tagaagtaca ttggttggtg gccatgcagg cggcgcagga gggcttaaaa atgattgatt
     1561 ataatgatcg tggacgttta aagtgggcag gtttttattt atctgatcat actgaaaaaa
     1621 ttgatgccga caatgaacaa cgagccaatc aaaatttggc aaaagaacaa atgacgactg
     1681 aagaggttgc ggaagtattg aacgctgcag tcataaaaaa tagatcagta tgtattcaaa
     1741 aagaagaacg aaacgctgaa ggatactatc cagaagatat tgttagcaag atcttaggta
     1801 gtgatgagct ggggatctac gttggaaaaa acaaagttca ctacgacgaa atacgtcata
     1861 tcgaatttta ccaggataat aaatggggtc gattagaatg aacattatcc agcaatatga
     1921 gctaaagtat ataacctttg atcagctatc agaagagatt tggggttatg gccagcgttt
     1981 gataaatgag gttggtgttg aacgcttctc tttctatgta gaagctgcag caggatacca
     2041 taaatacgaa tactatattg gctattaaag tgcaataaaa ttatatttct tttatgtttt
     2101 acattttgta tcaattctga taaaattacc tataggtaat taaaaaatat aaaaaaacta
     2161 gttacaacct taggacgttg taactagctt gaagcatact agagctaact aaaatagcac
     2221 tagaaagcaa tgttaaaccc gtccaacaaa cttgatttaa cattgagacc agcttattca
     2281 gctaaatata gttgaataag cttcttttgt tttaaaaaaa caaaaggtgt actacgaata
     2341 acgcaagtaa aagaaatact attacaacca gtaggctaca agtaagtatt actaaacatt
     2401 gcgtagttag aacctttgct ctattgtcta aagccacaaa attagagatc agctttatga
     2461 tgctaagagc gagctgcatc catgcggaca taggttcttt tgacactatt aattctcctt
     2521 tttaggataa gggaaattgc tagtagtttt ttcattagca ccatccccct tcatttgatt
     2581 gagtctagtg ctgctgtgat agtttgctgt ttaatatgct tacattctta ttataacata
     2641 aatgaaacaa acacatggtt agaggtcgta tcaatcaaaa tagtgtctca caaacgattt
     2701 attacttact cctgctgact aacggactgt tcttcagtaa ctgtaactat atgtttgtta
     2761 aaaccaccat tacctgtaga taa
//
LOCUS       DAZKLM010000162         2572 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3764
            SAMEA115706913-rid22582563.denovo.169, whole genome shotgun
            sequence.
ACCESSION   DAZKLM010000162 DAZKLM010000000
VERSION     DAZKLM010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706913
            Sequence Read Archive: ERR13196647
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2572)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2572)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287823.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287823.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,004
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,849
            CDSs (with protein)               :: 2,849
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 41 of 96
            Pseudo Genes (incomplete)         :: 52 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 15 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2572
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706913-rid22582563.denovo.169"
                     /isolate="VRE3764"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..391
                     /locus_tag="ACG842_002729"
     CDS             <1..391
                     /locus_tag="ACG842_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004612033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFQ0249204.1"
                     /translation="NLIDYLSPIENIRLVNKSADESILFELGLDKKQIKRNVMKLSGG
                     QQQRVAIARALVSDAPIILADEPTGNLDSVTAGEIINILKTLAKDRNKCVIVVTHSKE
                     VADSADIILELSGKKLKKVNKMNLEVE"
     gene            391..1767
                     /locus_tag="ACG842_002730"
     CDS             391..1767
                     /locus_tag="ACG842_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0249205.1"
                     /translation="MIKNAFAYVTRKSLKSLIIILVILSMSTLSLISLSIKDATDRAS
                     KETFANITNSFSMEINRQVNPGTPRGGGNVKGEDIKKISQTDSIDSYVKRINSVADLV
                     DYDIIETQETLANQSPERAKNFKRTVMLTGVNDSTKETKFVSEAYKLVEGKHLENEDK
                     NKILMHKDLAEKNNLKVGDKIKIKSNLFDADNEKGADETVEVEIKGLFDGHNSGVVSA
                     AQELYENTLITDIHTAAKVYGNTEDTAVYQDATFFVKGDKNLDSVIKDLGKLDINWRE
                     YNLIKSSSNYPALQQSISGIYSISNKLFFGSLIFAGVVVSLLLFLWMNARKKEIAVLL
                     SLGISKLEIFGQFLIEMVFISIPAFVGSYFLAQYTADKLGNNILNKVTGDIAKQIAKQ
                     SASSQLGGGAEAEGFNKTLSSLDINVLPKFMIYVVIFMSLVLLVSLIISSFNILRRNP
                     KELLIDNN"
     gene            1897..2310
                     /locus_tag="ACG842_002731"
     CDS             1897..2310
                     /locus_tag="ACG842_002731"
                     /inference="COORDINATES: protein motif:HMM:NF013068.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFQ0249206.1"
                     /translation="MARKKRNPDAEKLAESILNTYQPESVEDMQDVLKDVFGPLFEKM
                     LQGELNNHLGYDAHSKEPKKHDNRRNGYGNKTLKTSFGKVNIDVPRDREASFEPELIP
                     NRKRDVSDIADCRQKLFQTRNSSLEQLFIYFVSNF"
     gene            complement(2335..2572)
                     /locus_tag="ACG842_002732"
                     /pseudo
     CDS             complement(2335..2572)
                     /locus_tag="ACG842_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611630.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          957 a          355 c          461 g          799 t
ORIGIN      
        1 taatttaata gattatttat caccaattga aaatattaga ttagtaaata aatcagcaga
       61 tgaaagtatc ttgtttgaac taggtttaga taaaaaacaa ataaaaagaa atgttatgaa
      121 attatctggt ggacagcaac aaagagtagc tattgctaga gcattggtat cagatgctcc
      181 aataatacta gctgatgagc ctactggtaa tctagacagt gttactgctg gagaaataat
      241 taatatatta aagacattag ctaaagatag aaataaatgt gtaatagttg taacacatag
      301 taaggaagta gcagattctg cggatatcat tttagaacta agtggtaaaa agttgaaaaa
      361 agtaaataaa atgaatttgg aggttgaata atgataaaaa atgcatttgc ttatgtaact
      421 agaaaaagtt taaaatcatt aattatcata ttagttattt tatctatgtc aactttaagt
      481 ctcattagtt tatctattaa agatgctacg gacagagctt caaaagaaac atttgctaat
      541 ataacaaata gtttttctat ggagataaat agacaagtaa atccaggaac acctagaggt
      601 gggggaaatg taaaaggtga agatattaaa aagatatctc aaacagatag tatagactcc
      661 tatgtaaaaa gaataaacag tgttgcagat ttagttgatt atgatattat tgaaactcaa
      721 gagactcttg cgaatcaatc acctgaaaga gcaaagaatt ttaaaagaac agttatgtta
      781 actggggtta acgactcaac aaaagaaaca aaatttgtat cagaagcata taaattagta
      841 gaaggaaagc atttagaaaa tgaagataaa aataaaatct taatgcataa agatttagct
      901 gaaaaaaata accttaaagt tggagataag ataaaaataa aatctaattt atttgatgct
      961 gataatgaga aaggtgcaga tgaaacagta gaagtagaaa ttaaaggtct attcgatggg
     1021 cataatagcg gtgtagtaag tgcagcacaa gaactatacg aaaatacatt aatcactgat
     1081 attcatacag ctgctaaagt ttatggtaat accgaagata cagctgtata ccaagatgca
     1141 acattctttg taaaaggtga taagaatctt gatagtgtaa taaaagatct tgggaaatta
     1201 gatataaatt ggagagaata taatttgatt aaaagttcat caaattaccc tgcattacaa
     1261 caatctatat caggtattta ctcaatttca aataaattat tttttggctc attaatattt
     1321 gcaggagttg tagtttcatt attattattc ttatggatga atgctagaaa gaaagaaata
     1381 gcagtattac tatcattagg tatatcaaaa ttagaaattt ttggtcaatt cctaattgag
     1441 atggtattta tatcaatccc cgcattcgtt ggttcctatt tcttagctca atatacagct
     1501 gacaagcttg ggaataatat attgaataag gttactggtg atatagctaa acaaatagct
     1561 aaacaatctg catctagcca attaggtggt ggggcagaag ctgaaggatt taacaagaca
     1621 ttatcaagtt tagatatcaa tgtattacct aaattcatga tttatgtagt tatatttatg
     1681 agtttagtac ttcttgtatc attgattatt tcttcattca atatattaag aaggaatcca
     1741 aaagaattat taattgacaa taattaaaat tttcggtgct ttttagcacc aaaaatgaga
     1801 gtgtaatata aaccgtgtaa gtaaccatca atcctaggcg ggtggttgct tcacggtttt
     1861 tatttttcta aaataagatc aagaggtgat tacacgatgg ctagaaagaa aagaaatcct
     1921 gatgctgaga agttagctga atccattttg aatacttatc aacccgaatc tgtagaggat
     1981 atgcaagatg ttttaaaaga tgtgtttggt cccttatttg aaaaaatgct tcaaggagaa
     2041 ttgaataacc atttagggta tgacgcccat tctaaagagc caaagaaaca tgacaatcga
     2101 cgaaacggtt atggcaataa gacgcttaaa acgagctttg gtaaagtaaa cattgatgtt
     2161 cccagagacc gggaggcttc ttttgaacct gagttgattc ctaatagaaa acgagatgtc
     2221 tccgacatcg cagactgtag acaaaagctg ttccaaactc gtaactcgag cttggaacag
     2281 ctttttatct acttcgtttc gaatttttga acaaaaaaat aaaggctcct tgcctttcca
     2341 tagccaggtg gctaatttct ttaaattcat tgcagcaaac actagcatcg tatgcgtcgc
     2401 gactttttcc aagcctcgat acttggtcca acgcatccca tgcttttctt ttgcatccgc
     2461 aaatattcgt tcaatcgttt gtttccgttt tttgtaaatt gatttattca gatctgttag
     2521 gcgaagatgt tccacttcat ccatcagatc tccccaaaca tgtctcgtga tg
//
LOCUS       DAZKLM010000163         2497 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3764
            SAMEA115706913-rid22582563.denovo.170, whole genome shotgun
            sequence.
ACCESSION   DAZKLM010000163 DAZKLM010000000
VERSION     DAZKLM010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706913
            Sequence Read Archive: ERR13196647
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2497)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2497)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287823.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287823.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,004
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,849
            CDSs (with protein)               :: 2,849
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 41 of 96
            Pseudo Genes (incomplete)         :: 52 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 15 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2497
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706913-rid22582563.denovo.170"
                     /isolate="VRE3764"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(112..1050)
                     /locus_tag="ACG842_002733"
     CDS             complement(112..1050)
                     /locus_tag="ACG842_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HFQ0249207.1"
                     /translation="MKKIDEKLAAEISLKKPAVSNSRLSNSRLSGQTLGKSQPALSFD
                     AMTIVGNLNKTNAKKLSDFMSVEPQIRLWDILQTKFKAKALQEKVYIEYDKVKADTWD
                     RRNMRVEFNPNKLTHEEMIWLKQNIIDYMEDDGFTRLDLAFDFEDDLSDYYAMTDKAV
                     KKTVFYGRNGKPETKYFGVRDSDRFIRIYNKKQERKDNADVEVVSEHLWRVEVELKRD
                     MVDYWNDCFNDLHILKPAWATLEKINEQAMVYTLLHEESMWGKLSKNTKTKFKKLIRE
                     ISPIDLTELMKSTLKANEKQLQKQIDFWQREFRFWK"
     gene            1541..2497
                     /locus_tag="ACG842_002734"
                     /pseudo
     CDS             1541..2497
                     /locus_tag="ACG842_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108743.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
BASE COUNT          859 a          431 c          398 g          809 t
ORIGIN      
        1 ttttaaattt ttatatttag gaggaataat cctctgattt tttcatacgt tatgtcacct
       61 cgtaaatatt aattatactg aattagcaat ttttatcaaa taaaacttat tttacttcca
      121 aaacctaaat tcacgttgcc aaaaatcaat ctgcttttgc aattgttttt cgttcgcttt
      181 taaagtcgat ttcattaatt ccgttaaatc aattggagat atttctctaa tcaatttttt
      241 aaatttagtc ttagtattct tacttagctt tccccacata ctttcttcat gcaacaaagt
      301 ataaaccata gcttgctcat taattttttc taaagtagcc cacgcaggtt tcaagatgtg
      361 taaatcatta aaacaatcat tccagtaatc aaccatatct ctttttaatt caacttctac
      421 acgccataaa tgttcagaca caacttcaac atctgcgtta tctttacgtt cttgtttttt
      481 attataaatt ctaataaatc tatcactatc acggacacca aaatattttg tttctggctt
      541 gccattacga ccataaaaaa cagttttctt aactgcttta tcagtcattg catagtaatc
      601 gctcaaatca tcttcaaaat caaaagctaa gtctaatctt gtaaaaccgt catcttccat
      661 gtagtcgata atattttgtt ttaaccaaat catttcttca tgtgtgagtt tattgggatt
      721 aaattcaaca cgcatattac gtctatccca agtatctgct tttactttgt catattcgat
      781 ataaactttt tcttgaagtg ccttagcttt aaactttgtt tgaagtatat cccaaagtcg
      841 tatttgtggc tctacactca taaagtcaga tagcttttta gcattagttt tgttcaaatt
      901 tccaacgatt gtcatggcat caaaacttaa tgcgggttga gattttccca aagtttgacc
      961 acttaaccgg ctattactta accggctatt agagacggcc ggttttttta gtgagatttc
     1021 tgcagctaat ttttcatcaa ttttcttcat tttttatcgc tcctaaaata atttttggaa
     1081 cgaaaaaagg acgagtggaa tcgcaaaata aatttcgcct gtatccattc gtccattttg
     1141 ttttgtattc aaaatcaaaa attgatatac taatcatcga atgaataaca agtgtggttt
     1201 gttattccgg gcgagaacgt tggtagcgtt gctcgtcttt ttttatgtct ttttttcgtt
     1261 aaaactattt taaaactaaa tatatcttaa agcaaataaa ttcaaggggc taaaagcgcc
     1321 ccttaaaaac cccttgcctc tgaaaagttt tgtgtttcct ttccgtcggt ttcaaaagct
     1381 aatttactca aaaaaataga tcaagggtaa atgcactcac tgcgttcgcc cttgagctat
     1441 tttatttcgc aaattttaac gcatttgccg acgaaaagat aaaacacata aaaatatacg
     1501 cagatatatt taaaacgtct tagaacgcaa aattttacgt ttaaaagcta tgcaaggact
     1561 gtatgaagca aaattattga gttatccaag aacagataca ccatttatta cagagaacga
     1621 atttgcttat ttaaaagcga attttggcaa atatagcggt tttttaggac ttgatcttga
     1681 aatggttcaa acagagccta gaaagcgtta tgtggacggt agtaaggtac aggaacacca
     1741 cgccattatc ccaacaaaac aagtacctac cgaatctgca ttagcgaaaa tggacgattt
     1801 acaacgaaaa atttatgctt tagtcgttaa aacgaccgtt gccatgtttt tacctgatta
     1861 cttgtatgaa gaaaccaaga tacaaacgaa agtagctgat ttgctgtttc aatcgatcgg
     1921 aaagacacca aagcaagaag ggtggaaaat tcttttcaaa caacaaacca aagaagaaaa
     1981 agaggacgtt caaacgttac cactcgttat cattggcgaa cgtgccgaag ttggtgttaa
     2041 gagtgttgaa aaagaaacgc aaccgccaaa agcttttaca gagggtacat tattaactgc
     2101 tatgaaaacg gcgaataaaa cggttgatga tgaagaagca atcaagattt tacaagaagt
     2161 tgaggggatt ggaacagaag cgacaagagc aagtattatt gaagcgttaa aacaaaaaga
     2221 atatatccaa gtgattaaga ataagcttgt tgtaactgaa aaaggaaaat tattgtgcca
     2281 ggcagttgaa agtcagcacc ttttaacgag tgctgaaatg acggctaaat gggaaacgta
     2341 tttaaaaaaa atcggtaaaa gagaaggcaa tcaagagaac tttattacga atatcaaaaa
     2401 attcattgtt catttactgg aagctgtacc taacgatata gaaaaactaa atttttctga
     2461 ttaccaggaa cagaaagaaa aagaagcaga aaaaagt
//
LOCUS       DAZKLM010000164         2463 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3764
            SAMEA115706913-rid22582563.denovo.171, whole genome shotgun
            sequence.
ACCESSION   DAZKLM010000164 DAZKLM010000000
VERSION     DAZKLM010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706913
            Sequence Read Archive: ERR13196647
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2463)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2463)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287823.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287823.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,004
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,849
            CDSs (with protein)               :: 2,849
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 41 of 96
            Pseudo Genes (incomplete)         :: 52 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 15 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2463
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706913-rid22582563.denovo.171"
                     /isolate="VRE3764"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            163..2316
                     /locus_tag="ACG842_002735"
     CDS             163..2316
                     /locus_tag="ACG842_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan D,D-transpeptidase FtsI family
                     protein"
                     /protein_id="HFQ0249208.1"
                     /translation="MMKDFMKKILNTSWLEKIRKGAQEPKNKTFRRSHVPFRLNFLFF
                     IIFTLFVALIARLGYLQIVNGEEMEARVKSTSTITVQESTPRGMIYDSTGKALVTNKA
                     NQAITYTRGTQVTAKDLLEIATKLNSLIDVPVDENLTDRDKKDFWLADPKHLKEATKR
                     LSAKEKQLGNSEQYSAMVNKVTADEINLNEEQLKIATIFKRMNSAYELNTVYIKNSGV
                     TDQELAVVAERASELPGVSTGTDWTREYNAASSLKSILGSVTTEKQGLPADEAEKYLA
                     KGYSRNDRVGQSYLEKQYEDVLQGTKTQYEVSLDNEGNVSNQKEIFSGEKGSNLMLSM
                     NAEFQSKVEEILKRNYQTLINNGKAQYSPGAYAVAMNPQTGEVLAMTGFSHEQGSKEI
                     TENALGTITSAFDPGSVVKAGTLTAGWASNAISGNQVLIDEPIRLQGASEKSSVFNRS
                     GQVALDAVKASELSSNTYMIKVALKMLGLDYTPGMGLPSLDEEAKAYQQLRDSFKEFG
                     LGTTTGIDLPNESPGISRSVDYMKKFNADNGKEWYAPGNFTDLAFGQFDTYTPIQLAQ
                     YASTVANGGKRVQPHLVKAIYGNDENGNLGEVKKEIGTTVENTVNISAENMAILREGF
                     HQVVHGTDPYTTAKPLASAKMDLSAKTGTAETVAEGHPDIATVNSNIVAYGPTDNPEI
                     AISVVLPNLLDEQDHINLTIAKEIMDAYYDMFMAK"
BASE COUNT          843 a          428 c          545 g          647 t
ORIGIN      
        1 aaaatagttg gatatgtaac tttaagtgat tgctgtgaaa caaaaagttg attttatctt
       61 gaaattaggt aaagaatttc ttgctccgcc cattttttct tttttattaa gaagtttttg
      121 ggtacaatga agcatgaatg taaataggcg gaaggatagg aaatgatgaa agattttatg
      181 aaaaaaatat taaatacttc ttggttagaa aaaataagaa aaggtgcaca agaacctaag
      241 aacaaaacgt ttcgccggtc ccatgttcct tttcgcttaa attttctttt tttcattata
      301 tttacgttat ttgttgcgtt gattgctaga ttgggttatt tgcagatcgt gaacggcgaa
      361 gagatggagg cgcgagtgaa gtctacttct acgattacgg tccaagaaag tactcctcgc
      421 ggaatgatct acgactccac aggaaaagct ttagttacga ataaagcgaa tcaggccatc
      481 acatatacga gaggaacaca agttacggct aaagatttgc ttgaaatcgc aacgaagctg
      541 aactcgctga tcgatgttcc ggtagatgaa aatctgacag atagagataa aaaagatttt
      601 tggctggctg atcccaaaca tttgaaagaa gccacaaaaa gactttcggc aaaagaaaag
      661 caattaggga acagcgaaca atatagtgcg atggtcaata aagtcacagc tgatgagatt
      721 aatttaaacg aggagcaatt aaaaattgct accatcttta aacggatgaa tagtgcatat
      781 gaattaaaca ctgtctatat caaaaactct ggtgttactg atcaagagtt agctgttgtg
      841 gcagaacgcg cttctgaact tccaggagtt tcgactggca cagactggac acgagaatat
      901 aatgcagcaa gttcactgaa aagtattctt ggcagtgtga cgactgaaaa gcaaggactt
      961 ccagcagatg aagcagagaa atacctagca aaaggttatt ctcggaatga ccgtgtagga
     1021 caaagttatt tggaaaaaca atatgaagat gtcctgcaag gaaccaaaac ccaatatgaa
     1081 gtttcccttg ataatgaagg aaatgtttcg aatcaaaaag aaatcttctc tggggaaaaa
     1141 ggttctaatt taatgttatc tatgaatgca gaattccaat caaaagtaga agaaattttg
     1201 aaaaggaatt atcaaacatt gatcaacaac ggcaaagcac agtattcgcc tggtgcttat
     1261 gcagtggcta tgaatcctca gacaggagaa gtgctagcaa tgactggttt cagtcatgaa
     1321 caaggttcca aagaaatcac cgagaatgct ttaggcacga ttactagcgc ctttgatcca
     1381 gggtctgtag tcaaggctgg tactttgaca gcaggctggg caagtaacgc gatcagcgga
     1441 aatcaagttt tgattgatga gccgattcgt ctgcaaggtg cttcagaaaa aagctctgtc
     1501 tttaatcgaa gcggacaagt tgctcttgat gcagtcaaag catcggaatt gtcttctaat
     1561 acgtatatga tcaaagtggc gttaaagatg ttaggactag actatacacc tggaatggga
     1621 ctacctagtt tagatgaaga agctaaagct tatcagcaat tgagagactc tttcaaagag
     1681 tttggtcttg gtacaacgac tgggatcgat ttgccgaatg aatctcctgg gatctcacgt
     1741 tctgttgatt atatgaagaa gttcaatgcg gataatggca aagaatggta tgctccaggt
     1801 aactttaccg atttggcatt tggacagttt gacacatata caccaatcca attagctcaa
     1861 tatgcttcaa cagtcgctaa tggcgggaaa cgagtacagc cgcacctagt caaagcgatc
     1921 tatggcaatg acgaaaatgg aaacttgggt gaagtgaaga aagaaattgg cacaacagta
     1981 gaaaatacgg tcaatatttc tgcagaaaat atggcgatcc tccgtgaagg attccaccaa
     2041 gttgtccatg gtaccgatcc ttacacaaca gccaaaccat tagccagtgc gaaaatggac
     2101 ctttccgcaa aaacagggac tgcagaaacg gttgcggaag gtcatccaga tattgcgaca
     2161 gtcaatagta atatcgtggc atacggacca acagacaatc cggaaattgc catcagtgtt
     2221 gtcttgccga acttattaga cgaacaagac cacataaact tgacgattgc taaagaaata
     2281 atggacgcgt attatgatat gttcatggca aaataaaaaa gaggtcgtga aaaagccgct
     2341 tagtttcgag tatgaagaaa aattttgtga aaatagcttc tcacattttt gcttaaattt
     2401 gacttcatac cgagaaactg gcttttgaac accgtttact cggcaaaaaa ggttgtgaca
     2461 aaa
//
LOCUS       DAZKLM010000165         2459 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3764
            SAMEA115706913-rid22582563.denovo.172, whole genome shotgun
            sequence.
ACCESSION   DAZKLM010000165 DAZKLM010000000
VERSION     DAZKLM010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706913
            Sequence Read Archive: ERR13196647
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2459)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2459)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287823.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287823.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,004
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,849
            CDSs (with protein)               :: 2,849
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 41 of 96
            Pseudo Genes (incomplete)         :: 52 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 15 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2459
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706913-rid22582563.denovo.172"
                     /isolate="VRE3764"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            6..1150
                     /locus_tag="ACG842_002736"
                     /pseudo
     CDS             6..1150
                     /locus_tag="ACG842_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328567.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
     gene            1321..1779
                     /locus_tag="ACG842_002737"
     CDS             1321..1779
                     /locus_tag="ACG842_002737"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0043768 - S-ribosylhomocysteine lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009372 - quorum sensing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HFQ0249209.1"
                     /translation="MRMNVESFNLDHTKVKAPYVRVADRKTGAHGDVIIKYDVRFKQP
                     NKEHMDMPSLHSLEHLTAELIRNHADYIVDWSPMGCQTGFYLTVLNHESYEDILDVLE
                     ATMKDVLEATEVPASNETQCGWAASHTLEGAQALAREFLAKRDEWSEVEA"
     gene            1938..2408
                     /locus_tag="ACG842_002738"
     CDS             1938..2408
                     /locus_tag="ACG842_002738"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004602 - glutathione peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0043295 - glutathione binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HFQ0249210.1"
                     /translation="MSIYNFETTLEDGTVYSLKKYAGKPIIIVNTATKCGLAPQFKQL
                     EELYQAYQTQGLVILGFPSNQFKQELDSATEAAEACKTTYGVTFPMHQLIDVNGENTD
                     PLFTYLKNEAPGTVTKAIKWNFTKFLIDRNGKVIERFAPQTSPLKMTAAIESVL"
BASE COUNT          823 a          460 c          460 g          716 t
ORIGIN      
        1 gaaacatgca cattcaaaca aaattgatcc acggaggcat cagtgaagat cccacaacag
       61 gagctgtcag tgtacctatt tatcaaacat ccacttatcg gcaagatggc gtcggccagc
      121 ctaaacaata tgaatattca agatctggta atcctactcg ttttgcattg gaagaactga
      181 ttgctgattt ggaaggcggt gttcgaggat tcgctttttc ttctggtctt tctgggattc
      241 atgccgtttt ttctcttttt caagctggcg atcatatctt attaggagat gatgtctatg
      301 gaggaacatt ccgtctattt gacaaagttc ttactaaaaa tggtctcgag tacacgatca
      361 tagatacgag taatcttgat aaaatagaac aatccatcaa acccaataca aaagcacttt
      421 atcttgaaac acctagcaat ccgcttctaa agattactga tttagaaaaa agtgccactc
      481 tggctcatca acatggattg atagttattg ctgacaatac atttgctact ccttattttc
      541 aaagaccttt agatctcggt tctgatatcg ttgtccatag cggtaccaaa tatctaggcg
      601 gacacagcga tgtagtagct ggtttagtga cttctaacca aaagatctag ccgatcagat
      661 cggattttac caaaatgcta tcggagccgt acttggtccc caagatagct ggttacttca
      721 gcgcggaatc aagacactaa gtgttcgtat ggaggaacat caaaaaaatg cttttgtggt
      781 agctgatttt ctattttctc atccagcagt agaaaaggtc tactatccag gattacccga
      841 tcatgaactc catggagttg ctaagcagca gatgagcggc tttagcggaa tgatttcttt
      901 tactttaaaa aatgaagaat ctgctattcc atttgttgaa tcactccaac tatttacttt
      961 agcagaaagt ttaggtggcg tagaaagttt agtggaaatt ccttctgtca tgactcatgc
     1021 gtctattcca aaagaaaaac gagaagaggc tggcataaag gacggcttga tccgtctttc
     1081 tgtaggaatt gagtatggtc aggatctcat aaacgattta gctcaggcat ttgacagaat
     1141 caagaactag aagtcccaat aaacgactta gcagaaaaat ctaaaaaata tttcagattg
     1201 ctacaccttg gaaacgataa gtgttagaat caagtagaaa gacgtttttg taaatttcac
     1261 taaataattc tttagtgaaa agcatcaaaa cgaatgaaca aacagatagg agtcttacaa
     1321 atgagaatga atgtcgaaag ttttaatctt gaccatacaa aagtcaaagc accttatgta
     1381 cgtgtagctg atcgaaaaac gggtgcgcat ggcgatgtca tcattaaata cgacgtccgc
     1441 tttaaacaac caaataaaga acacatggac atgccaagtc tacattcact tgagcacttg
     1501 actgccgaac taatccgcaa tcacgcagat tacatcgtag actggtctcc aatgggatgc
     1561 caaacaggct tttacttgac tgtattgaat catgaaagct acgaagacat tttagatgtt
     1621 cttgaagcaa caatgaaaga tgttcttgaa gctactgaag tacctgcaag caatgaaaca
     1681 caatgtggct gggctgccag ccatacacta gaaggtgcac aagctttggc tcgtgaattt
     1741 ttagcaaaac gcgatgaatg gtctgaagta gaagcatagt ttctaaaaga acgtaaagat
     1801 gagaagcaat atagctatct tatcttcgta ttttattaaa tatttattgt ctgaaaagag
     1861 ttctaaacgg gaaaacaaat agctagtttt cagtttagcg ctctttttat tatataaaat
     1921 agaaaggaga aaactttatg tctatttata attttgaaac tactttagaa gacggcactg
     1981 tctattcatt gaaaaaatat gcaggcaaac cgataatcat tgttaacact gcaacaaaat
     2041 gcggtctagc acctcaattt aaacaattag aagaacttta ccaagcttat cagacacaag
     2101 gtctggttat cttaggcttt ccttccaatc aatttaaaca agagctagat tcagcgactg
     2161 aagcagcaga agcttgtaaa accacttatg gcgttacctt ccctatgcat caattgattg
     2221 acgtaaatgg agaaaataca gatcctcttt ttacttatct taaaaatgaa gctcctggta
     2281 ctgtcactaa agcaataaaa tggaatttta ctaaattttt gattgataga aatggcaaag
     2341 taatcgaacg atttgcacca caaacttctc cattgaaaat gaccgctgca attgaatccg
     2401 ttctttaact taataaaaat aaaaaaagat gaactatttc tagttcatct ttttgcctt
//
LOCUS       DAZKLM010000166         2415 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3764
            SAMEA115706913-rid22582563.denovo.173, whole genome shotgun
            sequence.
ACCESSION   DAZKLM010000166 DAZKLM010000000
VERSION     DAZKLM010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706913
            Sequence Read Archive: ERR13196647
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2415)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2415)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287823.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287823.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,004
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,849
            CDSs (with protein)               :: 2,849
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 41 of 96
            Pseudo Genes (incomplete)         :: 52 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 15 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2415
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706913-rid22582563.denovo.173"
                     /isolate="VRE3764"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(4..249)
                     /locus_tag="ACG842_002739"
     CDS             complement(4..249)
                     /locus_tag="ACG842_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0249211.1"
                     /translation="MDPIERLNSLSEEVTQTFHSDFVFLIDAEKIQHFPARNWTHDQI
                     IEELKKRFDHSLMVKPWHEHEVIYSPELPVFALIPKK"
     gene            complement(266..1117)
                     /locus_tag="ACG842_002740"
     CDS             complement(266..1117)
                     /locus_tag="ACG842_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFQ0249212.1"
                     /translation="MNSLTSTYRLSNGYEIPVVGFGTWQTPDGDVAVSSVKEALAAGY
                     RHIDTAQGYKNEESVGQAIKESGIPREEIFLTTKLWNANHSYELVMSSFEESLKKLQT
                     DYLDLFLIHWPNPVAFRDNWEQANADTWRAFEELYEAGKIKAIGVSNFLPHHLDTLAK
                     TAKIMPMVNQVFLAPGELQTTVVEYAKKHDMILEAYSPLGTGKIFDVPEMKQIAEAHD
                     KTIAQVALRWSLQHEFLPLPKSVTPSRIKENTELFDFELTEEEMKQIDQLDGVVGKAK
                     DPDTTQF"
     gene            1283..2287
                     /locus_tag="ACG842_002741"
     CDS             1283..2287
                     /locus_tag="ACG842_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HFQ0249213.1"
                     /translation="MDIYLKKAILHIIDRETGSPVFSQKELDLTKEYIRDFLQKKIQK
                     ISSAQTKTGQLAEESMIVESLKIAQENFVEASEAIVQRWFDIYQESEEAPSADVFVVL
                     YELDTVMYLSLLKVNYREAYTHFVEADEAGIDNRLILNRAILGAKTQKADEAFAVNLT
                     DWTYELIEKKYEFSGKKELYFSGRVIESVPAPSLEENVKVIQKVAKHLGKKFETEEFD
                     IMADLKEAVYDTIEEKGRLDHEMIAEKVFKENITAKMAFKEEVQEQGFVPEAPPVKEV
                     QEISEKKFGKQKFKLSNGIELIVPVDVYRNPDLIEFVNNPDGTISVMIKNVDEVLNRL
                     "
BASE COUNT          774 a          408 c          486 g          747 t
ORIGIN      
        1 tttctatttt ttcggtatca aggcaaaaac aggtagttca ggtgaataaa tgacttcatg
       61 ttcgtgccag ggtttgacca ttagcgaatg atcgaaccgt tttttcagtt cttcgatgat
      121 ttgatcatgt gtccagtttc ttgcaggaaa atgttggatt ttctctgcat cgatcaaaaa
      181 gacaaaatcg gaatgaaaag tctgggtaac ttcttccgag agagaattca agcgttcgat
      241 tggatccata aaatcacctt tcgacttaaa attgggtagt atctgggtct tttgctttgc
      301 cgactacacc atctaactga tcgatttgtt tcatttcttc ttctgttaat tcaaaatcaa
      361 ataattcagt attttctttg attctgcttg gtgtcactga cttaggaaga ggaaggaatt
      421 cgtgttgcag cgaccaacgc aaagcaactt gtgcaatcgt cttgtcatgt gcttcagcga
      481 tttgtttcat ttcaggcaca tcgaagattt taccagtgcc taatggacta tatgcttcga
      541 ggatcatatc atgttttttt gcatactcaa ctacagttgt ctgtaattcg cctggtgcaa
      601 ggaaaacttg attgaccatc ggcataattt ttgctgtttt tgctagtgta tctagatggt
      661 ggggtaagaa attgctcaca ccgattgctt tgattttacc tgcttcatac agttcttcaa
      721 atgctcgcca tgtatctgca tttgcttgtt cccaattatc acgaaaagca acagggtttg
      781 gccagtggat caaaaacaga tctaaataat ctgtttggag cttcttcaat gattcttcaa
      841 aagaagacat cacaagttca tagctgtggt ttgcattcca taattttgtt gtcaagaata
      901 tctcttctct aggaattcca ctttccttaa tcgcttggcc gacgctttct tcattcttgt
      961 atccttgggc agtatcgata tggcgataac ctgcagccag cgcttcttta acagaggaaa
     1021 cagcgacatc cccatcaggt gtttgccatg tgccgaatcc tactaccggg atttcgtaac
     1081 catttgatag acgataagtg cttgttaatg aattcatact ttcagctcct tttttattcg
     1141 ttcaactata agtgtagcgc atttttcgaa aaaaagaatc cattttgttt ctactcttga
     1201 aatttttgtt taaaagaaaa tgattttgat tctggtattt ttgatttttt ttccgtaaac
     1261 taagacagaa gggaatgaga aaatggatat ctatttaaaa aaagcaattc tgcatattat
     1321 tgacagagaa actggttctc cagtcttttc acaaaaagag ctggatctga caaaagaata
     1381 tattcgtgat tttcttcaaa agaagatcca aaaaatctct tctgctcaaa caaaaactgg
     1441 acaactagca gaagaaagca tgatcgttga atcacttaaa atcgctcaag aaaattttgt
     1501 ggaagcaagt gaagcaatcg ttcagcggtg gttcgatatc taccaagaaa gtgaagaagc
     1561 acctagtgca gatgtatttg ttgtcttgta tgaattagat acagtgatgt acttatcttt
     1621 attgaaagtc aattatcgtg aggcttacac tcactttgtt gaagcagacg aagcagggat
     1681 cgacaaccgc ttgattttga atcgagcaat tctaggggcg aaaacacaaa aagcagatga
     1741 agcattcgct gtgaatttga ctgattggac ctacgaatta attgaaaaaa aatatgagtt
     1801 ttctggcaaa aaagagcttt atttttctgg aagagtgatc gaaagtgttc ctgcaccttc
     1861 tttagaagaa aatgtaaaag tgatccaaaa agtagccaag catcttggaa aaaaatttga
     1921 gacagaagaa tttgatatca tggctgattt gaaagaagct gtctatgata cgatagaaga
     1981 aaaaggtcgg ttggatcatg agatgattgc ggaaaaagtc ttcaaggaaa acatcacggc
     2041 aaaaatggct tttaaagaag aagtacaaga gcaaggattt gtcccagaag cacctcctgt
     2101 aaaagaagta caagaaattt ctgagaaaaa atttggaaaa caaaaattca agctatcaaa
     2161 cgggatcgaa ctgatcgtac cagtagatgt atatcgcaac ccagatttga ttgaatttgt
     2221 gaataatccg gatggtacga tatccgtgat gatcaaaaat gtggatgaag tattgaatcg
     2281 tttataaaaa tgactagaat attaggtgtt caagtaccag tgatcagtta aatgaattgt
     2341 gtcaagagaa ggatcggaaa agacgaatat aacaaaaaaa gagcgtgaga caaaagtaaa
     2401 gaacactttt gtctc
//
LOCUS       DAZKLM010000167         2405 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3764
            SAMEA115706913-rid22582563.denovo.174, whole genome shotgun
            sequence.
ACCESSION   DAZKLM010000167 DAZKLM010000000
VERSION     DAZKLM010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706913
            Sequence Read Archive: ERR13196647
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2405)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2405)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287823.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287823.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,004
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,849
            CDSs (with protein)               :: 2,849
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 41 of 96
            Pseudo Genes (incomplete)         :: 52 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 15 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2405
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706913-rid22582563.denovo.174"
                     /isolate="VRE3764"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..103)
                     /locus_tag="ACG842_002742"
     CDS             complement(<1..103)
                     /locus_tag="ACG842_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HFQ0249214.1"
                     /translation="MNHFKGKQFKKDVIIVAVGYYLRYNLSYREVQEL"
     gene            complement(135..458)
                     /locus_tag="ACG842_002743"
     CDS             complement(135..458)
                     /locus_tag="ACG842_002743"
                     /inference="COORDINATES: protein motif:HMM:NF015824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
                     /protein_id="HFQ0249215.1"
                     /translation="MDSFQRLSLVNQFELLAKFEDQNRSYYERKIEILREGYEYHYDD
                     EIWSDLSEPFPKEDSRFVLDVLNMYRDINFSKSKLNLDENQITNSYYTHFGSVAKFSK
                     KSILV"
     gene            920..2302
                     /locus_tag="ACG842_002744"
                     /pseudo
     CDS             920..2302
                     /locus_tag="ACG842_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010726506.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          875 a          412 c          400 g          718 t
ORIGIN      
        1 acaattcctg aacttcacga tagcttagat tgtaacgcag gtagtaacca acagcgacaa
       61 taatgacgtc ttttttgaat tgtttgcctt taaaatgatt cattactctg tcctctctgt
      121 cttttttctc aattttacac taaaatagat tttttggaaa actttgcaac agaaccgaaa
      181 tgagtatagt atgaattagt aatttggttt tcatctaagt ttagctttga tttactaaaa
      241 ttgatatctc gatacatgtt caatacatct aatacaaatc gagaatcttc tttcggaaat
      301 ggctcagaaa gatcactcca aatttcatca tcataatgat actcgtaacc ttctctcaat
      361 atttcgattt ttctttcata ataagatctg ttttggtcct caaatttagc caataactca
      421 aattgattta ctagtgacaa tctctgaaaa ctgtccatta aatgtttctt cctatcttgt
      481 tcattaacaa aaaattataa gcccttacaa cgaatattat atattaaatt taattgtagg
      541 tgaacacgaa ttttataaat atttatggtg ttaatgacaa atgatatccg actcctcttc
      601 atacgttctg gactatttgc taatagatta aaatttcagc atctatttct gaccataaat
      661 aaatgagctg ccgaaaagct gccaaatcag acttgaaaat aaaggatcaa aaacttttaa
      721 gtttctgacc ccgtaactca cacttcaaaa tcttacttaa aaaggagtct gaaagaaaaa
      781 aagagtattc aaaatttttt tctaactagc tacatcacat ctatgcattg acactcaaag
      841 aaccagaata agtcatcaga aagcaagggg gtgcggacat aatcctggac gaaaaaataa
      901 acttaggagg aatgtccgtg tgttttccca caaagagcgt gtaagggcaa ttcagttatt
      961 tttaaaatac gattgctctt atgctgcaac cattcgagaa ttagggtatc cgtcgattgg
     1021 tgcactacgg aaatggtata aagagtattt ggaatccgga gagctccatc aggagcaccg
     1081 aaaaaaatca aagtattctg aagaacaaaa acgaatagcg gtgaatcatt attttgaata
     1141 tggtcaatgt tatgcacgaa ccattcgcat gcttggttat cctaatagag agtccttgcg
     1201 gcagcggtgt gaagaacttg caccaggagc tagaaaactc cgaaaaagtg cggtaaagtt
     1261 aacacaagat caaaaagaag ctgtgttgaa aaaattctat aaaccgcaaa ctaataggaa
     1321 aaatttagcc gaatctggag gaattagtag agttactcta tatcaatgga aaaacacggt
     1381 tcttggaaag gattttccat tgtgtatgac tttaaaaaat caggaaaaac aaaaagagct
     1441 acttttaaag gaagtagaag acttacaaaa acaagtacac caattacagc tcgaaaaagc
     1501 attactcgaa ggagcggctg aactgctaaa aaaagaaaaa ggcgtcaatc ttctttgtct
     1561 atcaaatcaa gaaaaaacga tattgattga tgccttacga aatcagttca ccttgaaaga
     1621 gcttcttcaa cagctccagt ttcctaaaag cagctatttt tatcaaaaac aagcacttga
     1681 gaaaccagat aagtattaca aagaacgtca aattaattat cacgattttc aatcataact
     1741 tctgtgcgta tggttaccgt agaattcacc aagctttaaa aaatatgggt aaaaagcttt
     1801 ctgaaaaagt tgtacgtaga ttgatgactg aggaaaatct atttgttaaa ttttctagac
     1861 gaaaaaagta ttcttcgtat gctggggaaa tttctcctgc acaccccaat ttattaaatc
     1921 ggaattttaa agcaaagaag ccaaatgaaa aatggttaac agatattact gaatttagaa
     1981 ttctcgcagg gaaaatctac ttatcaccac ttgtcgattg ttacgatggt gctattgtta
     2041 gttggactat tggaacaaag ccaacttgct tcactctatg tctaaaaaga gttattcacc
     2101 agataattca gcgtgcgaag gtttctttgg acgattaaaa aacgagatgt tttatcaacg
     2161 tgactggaag aatacaacaa tcaatcagtt tataaacaaa ttggatgatt atattcattg
     2221 gtataataat caacgaatta agttatcttt aggaaggcta agccctcttc aatatcggaa
     2281 gaagcaaaga tatatttgtt aaaaaggtcc aaaaaaaata tccgcacccc ctttttttgt
     2341 aaaaaactat tgcacaatac aagtagccta cggctatcga aatttccata actttcgagc
     2401 aagaa
//
LOCUS       DAZKLM010000168         2402 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3764
            SAMEA115706913-rid22582563.denovo.175, whole genome shotgun
            sequence.
ACCESSION   DAZKLM010000168 DAZKLM010000000
VERSION     DAZKLM010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706913
            Sequence Read Archive: ERR13196647
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2402)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2402)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287823.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287823.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,004
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,849
            CDSs (with protein)               :: 2,849
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 41 of 96
            Pseudo Genes (incomplete)         :: 52 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 15 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2402
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706913-rid22582563.denovo.175"
                     /isolate="VRE3764"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            76..>2402
                     /gene="esp"
                     /locus_tag="ACG842_002745"
     CDS             76..>2402
                     /gene="esp"
                     /locus_tag="ACG842_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="repetitive surface protein Esp"
                     /protein_id="HFQ0249216.1"
                     /translation="MVSKNNKRVFLEKTKKRVLKYSIKKLSVGVASVLIGVGLVLGTT
                     ELVKAQDEISPSSPLETAISSVQVGDKVASGNTFQENPGYTKNYNFSDLQFSPQELTG
                     DTLKGNTIGFEVYGKHNIAASTKNWEIRLQLDERLAKYVEKIQVDPKKGIGSSRRTFV
                     RINDSLGRPTNIWKVNYIRASDGLFAGAETTDTQTAPNGVITFEKSLDEIFKEIGIDN
                     LKTDRLMYRIYLVSHQDDDKIVPGIDSTGYFLTDSDDFYNSLDVSENNPDQFKHGSVS
                     AKYEKPNTQTKDGSGSTGANGAIILDHKLTKNYNFSYSSSAKGTPWYANYKIDERLVP
                     YVAGIQMHMVQADKVTYDVSFESGTKVADLAIERREGHENYGIGSITNNDLTKLIDFA
                     NASPRPVVIRYVLQLTKPLDEILEDMKATAQVEENKPFGEDFIFDSWLSDTNKKLIQN
                     TYGTGYYYLQDIDGDGNPDDKEESGDTNPYIGKPELEEVYDVDTTVKGKVFIHELAGT
                     GHKAQLVDKEGTVLAEKTIAPNEKDGAPISDTVEFEFTGVDSSKLIAKDELKIQIVSP
                     GFDKPEEGSTVIKESPKAVDKQTVVVGFKPDAKESIRNNKNLPEDAEYSWKTEPDTSN
                     VTDSTKGIVTVKIGNRTFDVDVEFAVKASQAMENDATYVPITTTPETTVQSGKPTFDK
                     PDVPLAKDAFSISDVYNKYFGNASVDANTGIVTFTPAKGVGESEPITGTIPIKIVYQD
                     GSVGTTDLAVTVSKDIYENPGENIPAGYHKVTFTAG"
BASE COUNT          870 a          376 c          488 g          668 t
ORIGIN      
        1 tctgtgaata atacgtatca gatttatctt ttattcttac taggaaagta attattaagg
       61 gagttgattt gaataatggt tagcaagaat aataagagag tatttctaga aaaaacaaag
      121 aaacgagtat tgaagtacag tatcaaaaaa ttaagtgttg gggttgcatc tgttttgatt
      181 ggtgttggtc ttgtcttggg aacaactgaa ctagttaaag cacaagatga aatttctcca
      241 agcagcccac ttgaaacagc gataagctcc gtacaagtag gtgacaaagt agcttcgggg
      301 aatacttttc aggagaatcc tggttatacg aaaaattata acttttcgga tttacaattt
      361 agtcctcaag aactaactgg agacacacta aaaggcaata caattggatt tgaagtttac
      421 ggaaaacata atattgcagc atcaactaaa aactgggaaa tccgtcttca attagatgaa
      481 cgattagcta aatatgtaga aaaaattcag gtcgatccaa agaaaggaat tggatcttct
      541 agacggacct ttgtaagaat taatgattcg ctgggtagac ctacaaatat ttggaaggtc
      601 aattatattc gtgcaagtga tggattattt gctggggcag aaacaaccga tacgcaaacc
      661 gctcctaatg gggtaattac gtttgaaaag agcctagatg aaatttttaa agagataggc
      721 atagacaatc tcaagaccga tcgtttaatg tatcgaatct acttggtaag tcatcaagac
      781 gatgataaaa ttgtaccggg tattgatagt acgggctact ttttaacaga ttcagatgat
      841 ttctataata gcttagatgt atctgaaaat aatcctgacc agtttaaaca tggatcagta
      901 agtgcaaaat atgagaaacc taatactcaa acaaaagatg gttcaggatc cacaggtgct
      961 aatggagcta taatcttaga tcataagtta acgaaaaatt ataatttctc atattcatct
     1021 tccgcaaagg gcacgccttg gtatgctaat tacaaaattg atgaacgatt agttccatat
     1081 gtagcgggta tacagatgca catggttcag gctgacaaag taacatatga tgtttcgttt
     1141 gaatccggaa caaaagtagc tgatttagca atcgagcgac gggaaggtca tgaaaattat
     1201 ggcataggtt caataactaa taatgattta actaaactta tcgattttgc taatgcaagt
     1261 ccacgtccag tcgttattcg atatgtttta caattgacta agccattaga tgaaatctta
     1321 gaagatatga aagcaacagc acaagttgaa gaaaataaac catttggtga agatttcatc
     1381 tttgattctt ggttgtcgga tacgaataaa aaattaatcc agaacactta tggaacaggt
     1441 tattattatt tgcaagatat tgatggtgat ggaaaccctg acgataaaga agagagcgga
     1501 gacacgaatc catatatcgg gaaacctgaa ttagaagaag tatatgatgt tgacacaaca
     1561 gttaagggga aagtattcat ccacgagtta gcgggaacag gtcacaaagc ccaacttgtt
     1621 gataaagaag gtactgtatt agcagaaaaa actatcgctc caaatgaaaa agatggggct
     1681 ccaatttcag atactgtaga atttgaattt acgggtgtag attcaagtaa attaatcgcg
     1741 aaagatgaat taaaaatcca aatcgtttct ccaggttttg ataaaccaga agaaggttca
     1801 accgttatta aggaatcacc aaaagcggtt gataaacaaa ccgtggtagt tggatttaaa
     1861 ccagatgcta aagaatcaat tcggaataat aaaaacttac ctgaagatgc agagtattca
     1921 tggaaaacag agcctgatac ttctaacgtt actgatagta cgaaaggtat tgtaactgtt
     1981 aagatcggaa atcgaacttt cgacgtggat gtagagtttg ctgtaaaagc ttctcaagct
     2041 atggaaaatg atgcaacata cgtacctata acaacaactc cagaaacgac agttcaaagt
     2101 ggaaaaccta catttgataa accagatgtt cctttagcta aagatgcctt ttcaatttca
     2161 gatgtctata ataagtactt cggtaatgca agtgttgatg caaacactgg tattgttaca
     2221 ttcactccag ctaaaggtgt aggagaatcg gagccgatta ctggaacaat tcctattaaa
     2281 attgtttacc aagatggttc tgtaggcacg accgatttag cagtaactgt aagtaaagat
     2341 atttatgaaa atccaggaga aaacattcct gcaggctacc acaaagtaac cttcaccgca
     2401 gg
//
LOCUS       DAZKLM010000169         2398 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3764
            SAMEA115706913-rid22582563.denovo.176, whole genome shotgun
            sequence.
ACCESSION   DAZKLM010000169 DAZKLM010000000
VERSION     DAZKLM010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706913
            Sequence Read Archive: ERR13196647
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287823.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287823.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,004
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,849
            CDSs (with protein)               :: 2,849
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 41 of 96
            Pseudo Genes (incomplete)         :: 52 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 15 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2398
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706913-rid22582563.denovo.176"
                     /isolate="VRE3764"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            207..2069
                     /locus_tag="ACG842_002746"
     CDS             207..2069
                     /locus_tag="ACG842_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFQ0249217.1"
                     /translation="MQQLTKRERQLLIYLVEMSEPVTIKKLAEKENVSVRTIKYDLDD
                     IREWLNERKQVLYSKRSQGIWMNISSSDRIKLKSELMDVNRFEIFADQSLRIKRLLIM
                     LLVTKEAITGMKMANALDVSKDTIMNDLDVLENNFLKEGLTLNRQARKGFWIIGEERL
                     VRLTIEEILQKEFTDYDIYKLMSLLLNGGETEYFEMYSATATPIQEVFNQVIIRMRHL
                     LEFENLEKLNYAELLNILIRVTIATVRLRKEATIGRYQLVAEQELDKNDLSYRVMQQV
                     FDYYQLPLFEDEYHYIYSDTFDTIHQQDVMKLVENVIYKVSKAINHSFYQDPQLLTNL
                     YAHLSMRLSRKKKFVNEYNPFKEDIKQKYPELFDAIKQAVRQEISEQNILINDSFVAY
                     IALHFLVSYEREEDLRSVRAVYICSTGLGVTSLIKQKINEELNNITIVAFASVLNAQE
                     MIATKDPDLVISIFPINGIDRPFIKVHPLPTEQDLKKIQQIIKKILASSNQQERHNLN
                     LRETSINKDTLEDFSKELILKAYTIYERLLVILEAELIQEYREAFLLHVFLMVHRITF
                     DQQYMAEGRQVDQIILDKGTLVGKIEAVFAENNLGINQSEISALFSYIKEGASS"
     gene            2069..>2398
                     /locus_tag="ACG842_002747"
     CDS             2069..>2398
                     /locus_tag="ACG842_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFQ0249218.1"
                     /translation="MKMSDEALKIIEESQNREKLKQVIDLIEERLVEHAIVPTELQWT
                     ILINHLNEMLKRSQRKETIPVVDRDLFKEVSKEAITISDEIVRSIGNLTDDEIYVLSI
                     HFETAKNN"
BASE COUNT          865 a          353 c          466 g          714 t
ORIGIN      
        1 aaaaatttga aaaagcgttt gattatttat attacgactt agtgcaataa aaaacgcttt
       61 ttaacagtac atgtagtcgt ctttataata gataaactct gtaatattag ttaaaaagct
      121 ataagttgca cttagttaag tgcaaacatt tcatattgtc gttagtttag aaaacgctta
      181 tattagcaat gtaaggagag atgataatgc agcagctaac aaaaagagaa aggcagcttt
      241 tgatttactt ggtggaaatg tctgaaccgg taacaatcaa aaagttggct gaaaaagaaa
      301 atgttagtgt tcggacgata aaatacgatc tagatgatat aagagagtgg ctgaatgagc
      361 ggaagcaagt cctatatagc aaacgtagtc agggaatttg gatgaatatt agtagctctg
      421 atcgaatcaa attaaaaagc gaattgatgg atgttaatcg ttttgaaata tttgcagatc
      481 aaagtctccg aatcaaacgt ttactaatta tgcttttagt aacaaaagaa gctattactg
      541 gaatgaaaat ggccaacgca ttagatgtca gcaaagatac gatcatgaat gatttagatg
      601 tgctggaaaa taattttctt aaggaaggat tgaccttgaa tcgtcaggcg cgaaagggtt
      661 tttggatcat aggagaagaa cgtctagttc gattaacgat tgaagagatt cttcaaaaag
      721 agttcacgga ttatgacatt tataaattaa tgtcattgtt attgaatggc ggtgagactg
      781 aatactttga aatgtattct gctactgcta cacctatcca agaggtattt aatcaagtaa
      841 tcattcgaat gcgacactta ctggaatttg aaaatctaga gaaactgaat tacgcggaat
      901 tactaaatat tctgattcga gtgacaattg ctaccgttcg cttacgcaaa gaggctacga
      961 ttggtcgtta tcaattggtt gctgaacaag aattagacaa aaacgatctt tcttatcgcg
     1021 tcatgcagca ggtctttgat tattatcagt tgccactgtt cgaggatgaa tatcattata
     1081 tctatagtga tacattcgac acgattcacc agcaagacgt catgaaacta gttgagaatg
     1141 tgatttataa agtaagtaaa gcaatcaacc attcttttta tcaagatcca caattgttga
     1201 cgaatctgta tgctcattta tccatgcgat tatcgagaaa gaaaaaattt gtcaatgaat
     1261 ataatccttt taaagaagat atcaagcaga aatacccgga attatttgat gcgatcaagc
     1321 aagcagttcg acaagaaata agtgaacaaa atattctgat caatgattct ttcgtggcat
     1381 atattgcctt acactttttg gtttcctacg aaagagaaga agatctacga agcgttagag
     1441 ccgtctacat ttgttcaaca gggttgggtg tgactagtct gatcaagcaa aagatcaatg
     1501 aagaattaaa taatatcact atcgtggcat ttgcttcagt attgaatgcg caagaaatga
     1561 ttgctaccaa agatcctgat ttggtgatta gtatttttcc aattaatggc atcgatcgtc
     1621 ctttcatcaa agtccatcca ttacccactg aacaggactt gaaaaaaatt caacaaataa
     1681 taaaaaaaat acttgcttct tccaatcagc aggaaaggca taacttgaat cttagagaaa
     1741 cgtccatcaa taaagatact ttagaagact ttagcaagga attgattttg aaagcttata
     1801 cgatttatga acgtttactt gtcattttgg aagcagaatt gatccaagaa tatcgagaag
     1861 cttttttgct tcatgtattc ttaatggttc atcgtatcac atttgatcaa caatatatgg
     1921 cagaaggtcg tcaagtagac cagattattt tagacaaagg aacactggta ggaaaaatcg
     1981 aagcagtttt tgcagaaaat aatttaggaa tcaatcagtc agaaatcagt gcattattct
     2041 catatataaa agaaggagcg tcaagttagt gaaaatgtca gacgaggctt taaaaattat
     2101 cgaggaaagt caaaatcggg aaaagttaaa acaagtgatt gatctaattg aagagcgatt
     2161 agtagaacat gctattgtac caaccgagtt gcagtggacc attctaatta atcacttgaa
     2221 tgagatgctg aaacgttctc aaagaaagga aacgattccc gtagtcgatc gggatttgtt
     2281 caaagaagta tccaaagaag ccataactat ttctgatgaa atagttagaa gtatcggtaa
     2341 tttaaccgat gatgaaatat atgttttgtc gattcatttt gaaacagcaa aaaataat
//
LOCUS       DAZKLM010000170         2227 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3764
            SAMEA115706913-rid22582563.denovo.177, whole genome shotgun
            sequence.
ACCESSION   DAZKLM010000170 DAZKLM010000000
VERSION     DAZKLM010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706913
            Sequence Read Archive: ERR13196647
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2227)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2227)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287823.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287823.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,004
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,849
            CDSs (with protein)               :: 2,849
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 41 of 96
            Pseudo Genes (incomplete)         :: 52 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 15 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2227
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706913-rid22582563.denovo.177"
                     /isolate="VRE3764"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            22..540
                     /locus_tag="ACG842_002748"
     CDS             22..540
                     /locus_tag="ACG842_002748"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HFQ0249219.1"
                     /translation="MPDYKKITFISSITLIIFLILILLVVLVPRIFGLTPYIIEDKEM
                     SPYYAQGNLIYVRPKNTNEILVGDIITYYENSGKKISTRRVIAVENNHEDFYTKADTK
                     TYIEPGVVKKRNIIGSPIFQIPYIGLVLSKTAFAWIQGLFFTIAFCLTGITAWNTFVE
                     LKKKRRRTQKFL"
     gene            668..1351
                     /locus_tag="ACG842_002749"
     CDS             668..1351
                     /locus_tag="ACG842_002749"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HFQ0249220.1"
                     /translation="MRNEMMHRPVVKEELLDFMRTQQKKLPGELGKLEEEAHVAEVPV
                     IPHETVVFLKFLLGQLQPERILEIGAAIGFSSSVMATTIGENAHVTTIDRFDVMIEKA
                     KKNYERLGLTDKVTLLEGQAADILPELSGPYDFIFMDSAKSKYIEFLPECLRLLRKGG
                     VLMVDDIFQGGTILLPDEEIPRGKRAIHRKLNEFLRVVMDHPDLTSTILPLGDGVILM
                     TKESETIEL"
     gene            1660..1733
                     /locus_tag="ACG842_002750"
     tRNA            1660..1733
                     /locus_tag="ACG842_002750"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1694..1696,aa:Arg,seq:tct)
     gene            1892..>2227
                     /locus_tag="ACG842_002751"
     CDS             1892..>2227
                     /locus_tag="ACG842_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 domain-containing protein"
                     /protein_id="HFQ0249221.1"
                     /translation="MTILSRILVTLVAIEFFYIMYIETVRTDSDTTSRVFKMSKEELS
                     RKSVQTLFKNQGVYNGLLGVGLLYGAYLSSASKEITSMLLISIFFVALYGSLTSDRWI
                     LLKQGGLALL"
BASE COUNT          745 a          346 c          453 g          683 t
ORIGIN      
        1 taaagaaaga agttgaaaac gatacctgat tacaaaaaaa taacttttat aagttctata
       61 acgttgatta tatttttaat tcttattctt ctcgtcgtct tggttccccg aatttttggt
      121 ttgactcctt atattattga ggacaaagaa atgagtccat attatgcaca aggaaatctg
      181 atatatgtac gaccgaaaaa tacaaatgag attttagtcg gagatattat tacatattat
      241 gaaaattcag gaaaaaaaat ctcgacgcgt cgcgtaatag ccgttgaaaa caaccacgaa
      301 gatttttata caaaagctga tacaaagact tatattgagc ctggagtagt aaaaaagaga
      361 aatattatcg gtagtcccat ctttcagatt ccatatattg gactagtcct ttcaaaaacg
      421 gcatttgcat ggattcaggg gttattcttt acgattgctt tttgcctaac aggaatcaca
      481 gcatggaata cctttgtaga gttgaaaaag aagcggagac gaacacagaa atttttgtaa
      541 gaatgttaaa aacataaatg tattccagag atccaacgac tcttctgaac atgaaacacg
      601 agaaaacact catgcagctt gtagatttag tcagttgttc gtataatgtg tgaagaggag
      661 gagtagtatg cgcaatgaaa tgatgcatcg tcctgtagtc aaagaagaac tattggattt
      721 tatgcgtacc cagcaaaaaa aattaccggg tgagctaggc aaactggaag aagaagctca
      781 tgtagcagaa gtaccagtta ttccccatga aacagttgta tttttgaaat ttttacttgg
      841 acagcttcag cctgaacgaa tcttggagat cggagcagct atcggttttt catcaagtgt
      901 catggccacg acaatcggag aaaacgctca tgttacgaca attgatcgtt tcgatgtcat
      961 gatcgagaaa gctaaaaaaa actatgaacg tctaggactg acagataaag tgactttgct
     1021 tgaaggtcaa gcagcagata tcttgccgga gttgtctggt ccgtatgact ttatttttat
     1081 ggatagtgca aaatcaaaat atattgaatt tttacctgaa tgcttacgtt tgttaagaaa
     1141 aggtggcgtt ttgatggtag atgacatctt ccaaggagga acgatcttgc tcccagatga
     1201 agagattccc cgtggcaaac gagctatcca tcgtaaatta aatgaatttc ttcgtgttgt
     1261 gatggatcat cccgatttga catcgacaat cttgccatta ggtgatggtg tgatcttaat
     1321 gacaaaggaa tcagaaacaa tcgagttgtg atacatgatt atggcagcct gtagataatc
     1381 gattgaatcg agggctcaat tcgaacttga tgtaaaagtt tctatatttg taagtcaatt
     1441 aaaggataca ggaaatctaa ataaacagga gtagcatgaa gtcaatgtag ctcctgtttt
     1501 caattgataa ccttagaaca gctgaaggag aatgtctcgt tttaaaagat tgatttagta
     1561 gttgggccaa aataattcat tatttgctaa ttaaataact atttcgtatt gacagaagca
     1621 atttaattat ctataattat tgatgttccg ctgattcttg tcatagtagc tcagctggat
     1681 agagcatccg ccttctaagc ggacggtcgg gggttcgaat ccctcctgtg acgctaaaaa
     1741 accgttagaa actgaggttt ctaacggttt ttctttgttt tttgtgccaa tattgtttgt
     1801 tttcaaaggc aaataaaata aaaaaacatc cttatcgagt acaattataa ggagcaaaaa
     1861 aataaaactc atcagaaaag aggaagaaga tatgacgata ctatctagaa ttttagtgac
     1921 tctagttgca attgaatttt tttatatcat gtatatcgaa acggtgagaa ctgattctga
     1981 cacgacaagt agggttttta aaatgagtaa agaagagtta agcagaaaat ctgttcaaac
     2041 attgtttaag aaccaaggag tgtacaatgg actattagga gtgggactac tttatggagc
     2101 ttatctttct tccgcatcta aagaaatcac aagtatgcta ttaatatcta ttttttttgt
     2161 tgcattatat ggcagtttga ctagcgatcg ttggatttta ctcaaacaag gtgggctagc
     2221 tctgttg
//
LOCUS       DAZKLM010000171         2224 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3764
            SAMEA115706913-rid22582563.denovo.178, whole genome shotgun
            sequence.
ACCESSION   DAZKLM010000171 DAZKLM010000000
VERSION     DAZKLM010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706913
            Sequence Read Archive: ERR13196647
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2224)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2224)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287823.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287823.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,004
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,849
            CDSs (with protein)               :: 2,849
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 41 of 96
            Pseudo Genes (incomplete)         :: 52 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 15 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2224
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706913-rid22582563.denovo.178"
                     /isolate="VRE3764"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            95..>2224
                     /gene="acm"
                     /locus_tag="ACG842_002752"
     CDS             95..>2224
                     /gene="acm"
                     /locus_tag="ACG842_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Acm"
                     /protein_id="HFQ0249222.1"
                     /translation="MKKCRNIILSMLFIITNITSLIPVHVYADAGRDISSNVTSLTVD
                     PTNITDGGNIKVKFSFDEKKQNIQPGDYLWINWPSEGNIRGEGFQKEIPLMIENKNVG
                     TLTVRKDSAQVVFNENIKNLDSVEGWGQFEIQARNVTDTGEENTGPFTVTSGDKTATV
                     NVTKPASGSSSSVFYYKTGDMLPEDTKHIRWFLNINNNGTYVEQPVKISDEIQSGQRL
                     DPSTFEINQIHLGEQKVYRGEEGIQQFLQDFPSATFNFSVTDNYIEITIPKNFVNLRK
                     IMVSYKTIIENPEQINFENHSEAWFKEFNKPAVDGESFNHTVKNISASGGVNGTVRGE
                     LKIFKYINDTEIGIPNVTFELRRADEQPIQGQSSILLTSNEQGEASIKGLQVGDYVVK
                     EKEAPNWIDFDPLSSNELKFSINENDTEGVSLPIYNKKKVTNITATKIWNGGTTPRPS
                     IYFKLFRASTNNWEPVPDAETKRLDNGITSVTWEDIQQYDDSGNEYTFKVQEVDQNGN
                     DYVPSGYQKIENGLVVTNENKEVISVSGQKTWEDNENQDGKRPTTITVNLLADGQPIQ
                     HKEVSEKDDWRYRFTNLPKYRDGQEIIYTVTEDNVPEYSTTIDGYNIKNSYTPEKTNI
                     SIPGSHITPWKNFPEKTEREKIRNLFSQKLQIFAHTGESSEQRSNYISKRAVPKQITN
                     KSKSILPKTSSKKSSFAVIIGLLIVTIC"
BASE COUNT          871 a          345 c          442 g          566 t
ORIGIN      
        1 gattggaaat gagtggggga ggaattgaag aaaggatttt tgagagatga tatagtaggt
       61 tttattttta gttaaaatag gggagataga aaaaatgaaa aagtgtagaa atattatttt
      121 atcaatgtta tttattatta ctaatataac atcactgatt cctgttcatg tctatgcgga
      181 tgcaggcaga gatatcagca gtaatgtcac ttcgttgacc gtggacccaa cgaatattac
      241 agatggagga aacataaaag taaaattctc gtttgacgaa aaaaaacaaa atatccaacc
      301 aggtgattat ctttggatca attggccaag tgaaggaaat attcgcggtg aaggattcca
      361 aaaagaaatc ccattaatga ttgaaaataa aaatgttgga actttaacgg tcagaaaaga
      421 ttctgcgcag gttgtgttta atgaaaatat taaaaaccta gactcagtag aaggttgggg
      481 gcaatttgag atacaggcca gaaacgtaac cgataccggt gaagaaaata cgggaccatt
      541 caccgtgacg agcggtgata aaacagctac tgtaaatgtg acaaagccag cttctggttc
      601 ttcttctagc gttttttatt ataaaacagg agacatgcta cctgaagata ctaagcacat
      661 tcgatggttt ttgaatatta acaataacgg cacttatgtt gaacaaccag ttaaaatcag
      721 tgatgaaatc caaagtggac aaagattaga tccaagtacg tttgaaatta atcaaataca
      781 cttaggtgaa cagaaggttt atagaggaga agagggaatc caacaatttt tacaagattt
      841 ccccagcgct acatttaatt tttcagtaac tgataattac attgaaataa ctataccaaa
      901 aaactttgtc aatcttagga aaataatggt gagttataag acgatcatag aaaacccaga
      961 acaaataaat tttgagaatc attcggaagc ttggtttaaa gaatttaata aacctgctgt
     1021 tgatggagaa agtttcaatc atacagttaa aaatatttca gcttctggtg gagtcaacgg
     1081 tacagttaga ggcgaattga agattttcaa atatatcaat gacacagaga ttggtatccc
     1141 aaatgtgact ttcgaattaa gacgagcaga tgaacagcct attcaaggac agtctagtat
     1201 cctgttgact tcgaatgaac agggagaagc tagtatcaaa ggcctgcaag taggagacta
     1261 tgttgtaaaa gaaaaagaag cacccaattg gattgatttt gatccactaa gttcaaatga
     1321 actcaaattc tccataaacg aaaatgatac agagggagtc agtttaccta tttataacaa
     1381 gaaaaaggtt acgaacatca cagcaactaa aatttggaac ggtggaacca ctccaagacc
     1441 gagtatttat tttaaattat tcagagcaag tacaaacaat tgggaaccag ttcctgatgc
     1501 agaaacaaaa cgattagaca atggaataac ttcagtgact tgggaagata tccaacagta
     1561 tgatgatagt ggaaacgaat atacgtttaa agtgcaagaa gtagatcaaa acggaaatga
     1621 ttatgtccct tcaggttatc aaaaaattga aaacgggcta gtcgttacaa atgagaataa
     1681 agaagtcatt tcagtctcag gacaaaaaac atgggaagac aatgagaacc aagacggcaa
     1741 acgaccgacg acgatcacag taaatctatt agccgacggt caaccgatcc aacacaaaga
     1801 agtaagtgaa aaagatgatt ggcgctatcg tttcacaaat ctgccaaaat acagagacgg
     1861 gcaagaaatc atctatactg taacggaaga caacgtgccc gaatacagta cgacgattga
     1921 cgggtataac atcaaaaaca gttatacacc ggaaaaaaca aatatatcta taccaggtag
     1981 ccatataaca ccgtggaaaa attttccaga gaagacagag agagagaaaa ttagaaatct
     2041 attctctcaa aaattgcaaa tatttgctca tacaggagaa tccagcgaac aaagaagcaa
     2101 ttatattagt aagagagctg tacctaaaca gataacaaat aaatcaaaaa gcattcttcc
     2161 taaaacaagt agtaaaaaat ctagttttgc tgtaatcatt ggtttattga tagtaactat
     2221 ctgt
//
LOCUS       DAZKLM010000172         2151 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3764
            SAMEA115706913-rid22582563.denovo.180, whole genome shotgun
            sequence.
ACCESSION   DAZKLM010000172 DAZKLM010000000
VERSION     DAZKLM010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706913
            Sequence Read Archive: ERR13196647
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2151)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2151)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287823.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287823.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,004
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,849
            CDSs (with protein)               :: 2,849
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 41 of 96
            Pseudo Genes (incomplete)         :: 52 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 15 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2151
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706913-rid22582563.denovo.180"
                     /isolate="VRE3764"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(31..1203)
                     /locus_tag="ACG842_002753"
     CDS             complement(31..1203)
                     /locus_tag="ACG842_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032495458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS1542 family transposase"
                     /protein_id="HFQ0249223.1"
                     /translation="MTQVHFTLNNEEVQSIIEHSVKDDVSKNILTTVFNQLMENQRTE
                     YIQADDYERSESRQSQRNGYYERDFTTRVGTLELKVPRTRDGEFSPTVFERYQRNEKA
                     LLASMLEMYVSGVSTRKVSKIVEELCGKSVSKSFVSSLTEQLDPMVNEWQNRSLSGTN
                     YPYLMTDVLYIKVREDHRVLSKSCHIAIGITEGGDREIIGFMIQNEESDDTWSIFFEY
                     LKERGLKGTELIISDAHKGLVSAIRKSFTNASWQRCQVHFLRNIFSSIPKKNSKPFRE
                     AVKAIFKFTDIELARTAKNALVGEYIDQPKYTKACEILDNGFEDAFQYTVIGNSHNRL
                     KSTNLLERLNQEVRRREKIIRIFPNRTSANRLIGAVLMDLHDEWLSSTRKYIKFDQ"
     gene            1367..1591
                     /locus_tag="ACG842_002754"
     CDS             1367..1591
                     /locus_tag="ACG842_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II bacteriocin"
                     /protein_id="HFQ0249224.1"
                     /translation="MKKKVLKHCVILGILGTCLAGIGTGIKVDAATYYGNGLYCNKEK
                     CWVDWNQAKGEIGKIIVNGWVNHGPWAPRR"
     gene            1611..1898
                     /locus_tag="ACG842_002755"
     CDS             1611..1898
                     /locus_tag="ACG842_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HFQ0249225.1"
                     /translation="MDFTKEEKLLNAISKVYNEATIDDYPDLKEKLFLYSKEISEGKS
                     VGEVSMKLSSFLGRYILKHKFGLPKSLIELQEIVSKESQVYRGWASIGIWS"
BASE COUNT          684 a          356 c          414 g          697 t
ORIGIN      
        1 taaaatacta tacaatgttt taccggtctc tcattgatca aacttaatat attttcttgt
       61 agaactgagc cattcgtcat gaaggtccat aaggacagct ccaattaatc gattggcaga
      121 cgttcggttg ggaaaaatcc gaataatctt ttctcttctg cggacttcct ggttcagtcg
      181 ttcaagaagg ttggtgcttt ttagccgatt atgactattt ccgataaccg tgtattgaaa
      241 ggcatcttcg aagccattat ccaatatttc gcaggctttt gtatatttag gctggtcgat
      301 atattcaccg actaaggcat tcttagctgt tcgagcgagt tcaatatccg taaacttaaa
      361 gatcgctttt actgcttctc taaacggttt tgaattcttt tttggaatgg aactgaagat
      421 gtttcttaaa aaatggacct ggcatctctg ccaacttgcg ttggtaaatg acttacgaat
      481 cgcagacact aggcctttat gggcatcaga aatgatgagt tctgtcccct ttaggccgcg
      541 ttcttttaag tattcaaaga agatggacca tgtgtcatca ctttcttcat tttgaatcat
      601 gaagccaatg atttcacggt cgccaccttc tgttatcccg atcgcaatat ggcagctttt
      661 agaaagcact cgatggtcct cacggacttt tatgtagaga acatcagtca tcagataagg
      721 ataattcgta cctgagagtg aacggttctg ccattcgttg accatcgggt ctaactgctc
      781 agtcaggcta gaaacaaaag atttcgatac agattttcca catagctctt caacaatctt
      841 tgaaacttta cgtgtcgaaa cgcccgaaac atacatctca agcattgaag cgagcagtgc
      901 cttttcattt cgctgataac gctcaaacac cgtcggtgaa aattcaccat cacgtgttct
      961 aggcactttt aattcgagtg tacccacacg agtcgtaaag tctcgctcat aatagccatt
     1021 tctttgactc tgacgacttt ctgaacgttc atagtcatcg gcttgaatat attctgttcg
     1081 ttgattttcc atcaattggt tgaaaacagt ggttaaaata tttttagaaa catcatcttt
     1141 taccgaatgt tcaataatac tttgaacctc ttcgttgttc agtgtaaaat gtacttgggt
     1201 catgtaacgt cctcctgggt atgtttttgt ggttaaaaac attgtaccgt aaaagggctg
     1261 ttacatggcc ttttatcttt tacacaatta tatggacttt atctaaaatt actaagatta
     1321 tttaatataa caagcaattt taaaaaaacg aaaaggaaaa acaatcatga aaaagaaagt
     1381 attaaaacat tgtgttattc taggaatatt aggaacttgt ctagctggca tcggtacagg
     1441 aataaaagtt gatgcagcta cttactatgg aaatggtctt tattgtaaca aagaaaaatg
     1501 ttgggtagat tggaatcaag ctaaaggaga aattggaaaa attattgtta atggttgggt
     1561 taatcatggt ccatgggcac ctagaaggta gtggtaggga ggttattata atggatttta
     1621 ctaaagaaga aaaactttta aatgcaatta gtaaagtata caatgaagca actatagatg
     1681 actatcctga cttaaaagaa aagctctttc tttattctaa agaaatcagt gagggaaaaa
     1741 gtgttggtga agttagtatg aaattaagta gttttcttgg aagatatatt ttaaaacata
     1801 aatttggatt acctaaatct ttaatagaat tacaagaaat tgttagtaag gaatctcaag
     1861 tatatagagg atgggcttct attggtattt ggagttaatt ctttggaata aaatgaaaat
     1921 gggactgaag aatcaattct gattcatcag tcccataaat tatagagccg aaaaattatc
     1981 atatttatag atttattaat agtaaatggc ataagaatct ttgaacaacg aaaaaaatgt
     2041 agctctacct aattattgat ttgcaacaat ttcaccgaag gtgaaacaag cgaaacttta
     2101 caaaaagtgg ctagcatttg ctagtctttt ttgtattgtg gagagtgggg g
//
LOCUS       DAZKLM010000173         2108 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3764
            SAMEA115706913-rid22582563.denovo.181, whole genome shotgun
            sequence.
ACCESSION   DAZKLM010000173 DAZKLM010000000
VERSION     DAZKLM010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706913
            Sequence Read Archive: ERR13196647
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2108)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2108)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287823.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287823.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,004
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,849
            CDSs (with protein)               :: 2,849
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 41 of 96
            Pseudo Genes (incomplete)         :: 52 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 15 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2108
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706913-rid22582563.denovo.181"
                     /isolate="VRE3764"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            278..820
                     /locus_tag="ACG842_002756"
     CDS             278..820
                     /locus_tag="ACG842_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0249226.1"
                     /translation="MLLKVAIKCYCVKNKGGIIYEKIVPRGACLLLALCLSLSAVGCQ
                     KSNENNKIKEGQSISSEEEGMSINANGKNETFKPSDYTLEAKKEYVYEYLGLKFKLSD
                     KFRNYIADKKIAMLDDQSPIDKELKYAILTFEKMTEEQKNAVIEKMGDGYKNWQNELE
                     RIGTIGIFEKNTSEEKNLKL"
     gene            976..1425
                     /locus_tag="ACG842_002757"
     CDS             976..1425
                     /locus_tag="ACG842_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HFQ0249227.1"
                     /translation="MLSEKTDLENTEAFNKESVKDLRKLPTKDINGKDFTSKDFEKYD
                     LTMVNVFATWCTACVKEIPDLVEVQNEMKSKGVNIVGVVTDPVDDNGENKEAIEKSKL
                     IHEKTKASYPFLMPDGMATLFLTKIIRCCECCGVMATIALDNEHPRI"
     gene            complement(1455..>2108)
                     /locus_tag="ACG842_002758"
     CDS             complement(1455..>2108)
                     /locus_tag="ACG842_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEfa8 family transposase"
                     /protein_id="HFQ0249228.1"
                     /translation="GRIMKRRGLTSTYTIAHFKGQRTACNEAKTANVLDRTFTQEQPL
                     EAIVTDLTYVRVGKKWHYICLILDLFNREIIGYSCGEKKDASLVKEAFGRIPYSLTDV
                     KLFHTDRGKEFDNQTIHEILNGFGITRSLSRKGCPYDNAVVESTYKSVKVEFVHQYQF
                     ETLAQLRLELFDYVHWWNYLRLHGTLAYETPIQIRQQRLAKRILDNERGSDTSGEAA"
BASE COUNT          751 a          335 c          397 g          625 t
ORIGIN      
        1 catgaaaaat cctccagtgt gttttttatt attctacaca ccttattttt tctgtctagt
       61 ctagtgtagc cgattcaaat tgatgaagca gatcccaaag ggctgcttca tcaatttttt
      121 cgtaagtgtc cgtacactta ctgtgcttgc tcatatatta atcgatcata tactaatcgt
      181 gtaagaacaa ctttaagcat aatacttata ttgttatacc atagtttgga aaaataaaaa
      241 attatatttc atcaacgttt cataaaaggt gtgtagaatg ttattgaaag tagctataaa
      301 atgctattgt gtaaaaaata aaggaggaat aatttatgaa aaaatagtac caagaggagc
      361 atgcctatta cttgcattat gtttgagctt aagcgcagtt gggtgtcaaa aaagtaatga
      421 aaacaataaa ataaaagaag gacaatccat aagttctgaa gaggaaggca tgtcaattaa
      481 tgcaaatggc aaaaatgaaa cttttaagcc atcagattat acattggagg caaagaaaga
      541 atatgtttat gaatatttag ggcttaaatt taaactctca gacaaattta gaaattatat
      601 agctgataaa aagatagcga tgttagatga tcaaagtcct atagataaag aactaaagta
      661 tgctattttg acatttgaga aaatgacaga agaacaaaag aatgcagtta tagaaaagat
      721 gggcgatgga tataaaaatt ggcaaaatga gcttgaaaga attgggacta ttggtatatt
      781 tgaaaaaaat acatcagaag aaaaaaatct aaaattataa aatgtgatac tcatactaaa
      841 ataggagttt caagtgatgg aaaatacgat tgctatttaa gcacaaatag cggagctgaa
      901 agtaatcttc tggatgaatt aaaaagaact gagattcaaa ttatagataa aaaagaacgt
      961 cctaaaaatg gttttgtgct atcagaaaaa actgatttag aaaatacaga agcatttaac
     1021 aaagaatcgg ttaaagattt acgtaaacta ccaacaaagg acataaatgg caaggatttt
     1081 acaagtaaag attttgagaa gtatgatctt actatggtaa atgtatttgc aacttggtgt
     1141 acagcttgtg taaaggaaat tcctgatttg gttgaggttc agaatgaaat gaaaagcaag
     1201 ggtgtaaata tcgttggagt tgttacagat cctgttgatg ataacggtga aaacaaggaa
     1261 gcaattgaaa agtcaaagtt aatacatgaa aagacaaagg cttcatatcc tttcttaatg
     1321 ccagatggaa tggcaacact ttttttgaca aaaattataa ggtgttgtga atgctgcggt
     1381 gttatggcta cgattgccct tgacaatgag catcctcgga tctgattttc tccggcagaa
     1441 gcacaatcat tcaattacgc tgcctctcca gaggtatcag atccgcgctc attatcaagg
     1501 attcgcttcg ccaatctctg ttgtcgaatt tggatcggtg tttcatacgc caacgtgcca
     1561 tgtaagcgta gatagttcca ccaatgcaca taatcaaaca attctagacg tagctgtgcc
     1621 agtgtctcaa attggtattg atgcacgaat tctactttga cagatttata ggttgattcc
     1681 acaacggcat tatcatacgg acaacccttc ctactcaatg aacgagtaat tccaaaacca
     1741 ttcagaatct catgaatggt ttggttatca aattcctttc cccggtctgt atgaaaaagc
     1801 ttgacgtctg ttaaagaata cggtatccgt ccaaaggctt cttttaccaa tgaggcatct
     1861 ttcttctcac cacaggaata accaataatt tctcgattaa acaaatcaag tattaagcag
     1921 atataatgcc actttttccc cacgcgaaca taagtaagat ccgtaacgat ggcttccaat
     1981 ggctgttctt gtgtaaaggt ccgatctaat acattcgctg ttttcgcttc attacaagct
     2041 gttcgttgcc ctttaaaatg agcgatcgta taggtagatg tcaatccgcg gcgtttcatg
     2101 attcgacc
//
LOCUS       DAZKLM010000174         2075 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3764
            SAMEA115706913-rid22582563.denovo.182, whole genome shotgun
            sequence.
ACCESSION   DAZKLM010000174 DAZKLM010000000
VERSION     DAZKLM010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706913
            Sequence Read Archive: ERR13196647
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2075)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2075)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287823.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287823.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,004
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,849
            CDSs (with protein)               :: 2,849
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 41 of 96
            Pseudo Genes (incomplete)         :: 52 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 15 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2075
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706913-rid22582563.denovo.182"
                     /isolate="VRE3764"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..464
                     /locus_tag="ACG842_002759"
     CDS             <1..464
                     /locus_tag="ACG842_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0249229.1"
                     /translation="LVYFQGGNLMIKKLFLLSILTLAMAGCSDSGLATELITKSTEAT
                     TEELKCGNDHSDYRGLNRRCMGILGRSNCKHRCSNRRRCRRSNRKIKRKIKRSNLCCA
                     SYTDVEDAVLLNHVRRCSFMSREVGDEPKLEYAFSFAREDEDNPKTYDILA"
     gene            complement(525..890)
                     /locus_tag="ACG842_002760"
     CDS             complement(525..890)
                     /locus_tag="ACG842_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02328 family protein"
                     /protein_id="HFQ0249230.1"
                     /translation="MRLWHEKLISKLPRQQLLGQHRECCALRGNGWGKKHATVDYVFH
                     YSPYRLFQYHQLVMDEMEKRGYSPAPEWKNPMYRGKSCEAFFDLPTVERNVPIYPEHD
                     EVYLAECLANLEQKGIYLT"
     gene            complement(966..1211)
                     /locus_tag="ACG842_002761"
     CDS             complement(966..1211)
                     /locus_tag="ACG842_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFQ0249231.1"
                     /translation="MSIEEAILLDTLRCRLNFLRTGRSLSQVAVSSALSIPLFKYRKY
                     EEGEEIPSIIELIYIADFYDISVDYLIGRSETASKLK"
     gene            complement(1281..1535)
                     /locus_tag="ACG842_002762"
     CDS             complement(1281..1535)
                     /locus_tag="ACG842_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0249232.1"
                     /translation="MITLVRVLFWVPAVALVASIVYLMNWNKERFYLAILTLPAIYFM
                     WKVFNYNYFEPDSVFIEELSGLVLSLLIVILYLIRLNKKH"
     gene            complement(1699..2022)
                     /locus_tag="ACG842_002763"
     CDS             complement(1699..2022)
                     /locus_tag="ACG842_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0249233.1"
                     /translation="MLGVIILPFIAILFDLVAAAAYFLQLNYQTSGILFIGMIFQGVI
                     TLLLLVMMLSYKGKRYARIQTKVFVKYVSIRYGIIILSFLVNAIVLFLYVLNYLDINP
                     LIFSR"
BASE COUNT          773 a          357 c          365 g          580 t
ORIGIN      
        1 ttttagtcta ttttcagggg gggaacctta tgataaaaaa actattttta ctatcaatat
       61 taacgctagc aatggcaggt tgttctgact ctggcttagc aactgaactg atcacaaaat
      121 caactgaagc cacgacagag gagttaaaat gtggaaacga tcacagtgac tacagaggct
      181 taaatcgacg atgcatggga atattaggca gaagcaattg taagcatcga tgtagtaatc
      241 gaagaaggtg tagaagaagc aacagaaaaa tcaaaagaaa gatcaaaaga agcaatctgt
      301 gctgtgcatc gtatacagac gtcgaagatg cggtactttt aaaccacgtc aggagatgtt
      361 catttatgtc tagggaagtt ggcgatgaac caaaactaga gtacgctttt agttttgcac
      421 gtgaggatga agataaccct aaaacttacg acattttagc ttgatctaag atggttaaag
      481 ctttttaatg aagtaagagg agatagaagg cttttctagt tatattaggt aagataaatt
      541 cctttttgct ccaaatttgc taaacattct gctagataaa cttcatcgtg ttccggataa
      601 atcggcacgt tgcgctctac agtaggtaaa tcaaaaaatg cttcgcatga tttgccacga
      661 tacataggat ttttccactc tggtgctggg ctgtaaccac gtttttccat ttcatccatg
      721 acaagctgat gatattggaa taatctataa ggagaatagt gaaaaacata atccacagta
      781 gcatgtttct ttccccagcc atttcctctt aatgcacagc attcccgatg ttgtcctaaa
      841 agctgttgac gaggaagctt tgaaatcaat ttttcatgcc ataatcgcat agaaatgtct
      901 cctttatata tagattgtta accttagacg ttaaatgaaa aaaccagcac caaggctgga
      961 aaaaatcact tcagtttgct tgctgtttct gagcgaccaa ttaggtaatc aacggaaata
     1021 tcgtaaaagt cagcgatata gattaattca atgatgcttg gaatttcttc tccttcttcg
     1081 tacttacgat atttgaataa aggaatactt aacgcgctag aaacagctac ttgagataaa
     1141 gatcggccag ttcttaagaa attcagacga caacgtaaag tatccagtaa aatagcttct
     1201 tcgatactca tttaatcacc tttcattata gtagaatagt tataatgact atagaaagct
     1261 acacatgcaa tcaatagtac tcaatgtttt ttatttaaac gtatcaaata aagaatgacg
     1321 atcaataaag acaaaaccag tcctgataat tcttctataa aaacactatc gggttcaaag
     1381 tagttgtaat taaaaacttt ccacataaag tagatagccg gaagagtcaa aatagctaaa
     1441 taaaaccgtt ctttgttcca attcataaga taaacaatac ttgcgaccag tgcgaccgca
     1501 ggcacccaaa acaaaactct caccaacgta atcatgtaac ggactcctca acgattcata
     1561 taataaaaga taacatgtta ttgcatacat cacaaatgaa atgctaggaa aataaaagaa
     1621 gaatagatca atgaaaaatg aaaaagcaga acagttttct gctgataagc aggaactgtt
     1681 ccgctgaatg tgtcttatct atcgagaaaa aatcaatgga ttgatatcta agtaattcaa
     1741 tacatataaa aagaggacga tcgcattcac taaaaaggaa agaatgatga tcccatagcg
     1801 aatggaaaca tatttaacaa atacttttgt ttgaatacgg gcatatcttt ttcctttata
     1861 gctgagcatc atgacaagaa gcaaaagggt aatcacacct tgaaaaatca ttccaataaa
     1921 cagaattcct gaggtttgat aatttaattg gagaaaataa gctgctgccg caactaaatc
     1981 gaataaaatc gcaataaatg gcaaaataat cactcctaac attgagaata aataaaagaa
     2041 taacattttt tctaaaataa gcaaataaaa attaa
//
LOCUS       DAZKLM010000175         2003 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3764
            SAMEA115706913-rid22582563.denovo.183, whole genome shotgun
            sequence.
ACCESSION   DAZKLM010000175 DAZKLM010000000
VERSION     DAZKLM010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706913
            Sequence Read Archive: ERR13196647
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2003)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2003)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287823.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287823.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,004
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,849
            CDSs (with protein)               :: 2,849
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 41 of 96
            Pseudo Genes (incomplete)         :: 52 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 15 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2003
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706913-rid22582563.denovo.183"
                     /isolate="VRE3764"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..378
                     /locus_tag="ACG842_002764"
     CDS             <1..378
                     /locus_tag="ACG842_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HFQ0249234.1"
                     /translation="KNVSTEDFFNYCRLEESGVTVTSGTDLPLFITDIPESIIRAVFR
                     RFPEGEEVWEKETSFTTTELLKSFTSNSFKVNGLSEYGEIKVGKSASLAVFSEDLCTD
                     DWQKVMAAEVIATYLDGDLVYKK"
     gene            426..1826
                     /locus_tag="ACG842_002765"
     CDS             426..1826
                     /locus_tag="ACG842_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HFQ0249235.1"
                     /translation="METMKRKFTFPHSYTLIFILIILAAILTWIIPSGQFETVVENVA
                     GMEKTTVVPGTYHQIDKADYGQGFSSILMAPAEGIISAVEVVAFVLIVGGAFGIILKT
                     GAIDRGLFTLAKVLGDKGILVIPMSMILFSLGGSTFGMSEEVIPLFAVFISLMFSLGY
                     DSMTAILILFLSTQAGYVGSTINPFNVLIAQGVAGVYGNPLLWFRMLSWIVITGISIV
                     FTMLYASRVKKNPLSSIVYESDLKVKHKFQNFDTEKEFSTQDKLIIAGFIVGLGIMIW
                     GIISKGWYMVEISALFLGLGLFAGGIARFSQKEIAEAFVEGCSDFAYAAVIIGLARGV
                     LVILEGGMIIDTILNSLANLLGGLPKFLFTTILLISQVIVSFFVPSSSGAAALTMPVM
                     APLGDLMNVSRDVVVLSNQFGNGLMNIISPTSGVLLAGLAIAEINFSKWFKVGFKIFA
                     ILTLVSAVLLYIATLF"
BASE COUNT          555 a          339 c          432 g          677 t
ORIGIN      
        1 aaaaatgttt ccacagagga cttctttaat tattgtcgat tagaggaaag tggtgtaaca
       61 gtcactagcg gtacagattt accgctgttt attactgata ttcctgaatc gattataaga
      121 gcagtattcc gtcgttttcc tgaaggagaa gaagtctggg agaaagaaac aagcttcacg
      181 acaaccgagc tgctgaaaag ttttacaagc aacagcttca aagtaaatgg tttatcggag
      241 tacggagaaa taaaagtagg gaaaagcgcc agcttagctg ttttcagcga ggacctctgt
      301 actgatgact ggcaaaaagt gatggctgcg gaagttatcg ctacctatct tgatggtgac
      361 ttagtttaca agaaataatg cttttcgata taacgttata aaacgaatta aagagaggtt
      421 aaaaaatgga aacaatgaaa agaaaattca ccttcccaca tagttataca ttgattttta
      481 tcttaattat tttggctgca attttaactt ggataattcc tagtggtcag tttgagacag
      541 ttgttgaaaa cgtcgcaggt atggaaaaaa caacggttgt tccgggaacc tatcaccaaa
      601 tcgataaggc agattatgga caaggttttt cctctatact aatggcacca gctgaaggga
      661 ttatttcagc tgttgaggtt gttgcttttg ttctcatcgt tggtggtgcc ttcgggatta
      721 tcctaaaaac gggagccatc gatcgagggc tgtttacctt agcaaaagtt ctgggagaca
      781 aggggatctt agttattccg atgtcgatga ttctattcag cttaggagga tcaacctttg
      841 gcatgagcga ggaagtcatt cccttatttg cggtgtttat ttctctgatg ttttctcttg
      901 gctatgattc catgactgcg atcttgattc tatttttaag tacacaagcg ggatatgttg
      961 ggtcgacgat taatcccttc aacgttttaa tcgcacaggg agtagctggc gtttacggaa
     1021 atcccctgct ttggtttcgg atgctatcct ggattgtcat tactgggatt tcaattgttt
     1081 tcaccatgct ttatgctagc agagtcaaaa agaatccact gtcctctatt gtttatgaga
     1141 gtgatttaaa ggttaagcat aagtttcaaa attttgacac tgaaaaagag ttttcaacac
     1201 aagataaact aattatcgcc ggttttatcg taggtttagg aatcatgatt tggggtatca
     1261 tttccaaggg atggtatatg gttgaaattt ctgcactatt cttaggatta ggtctttttg
     1321 ctgggggcat cgctagattt tctcaaaagg aaattgctga agcttttgtc gaaggctgtt
     1381 cagatttcgc ctatgctgct gtaatcattg ggctagctag aggagtcttg gtaatcttgg
     1441 aaggtggaat gattattgac actattctaa acagcttggc taacctttta ggtggtcttc
     1501 caaaattcct gttcactacg attctactca tttctcaagt aattgtttcc ttttttgttc
     1561 catcctcctc aggagctgcg gcattaacga tgccggttat ggcaccattg ggtgatctaa
     1621 tgaatgtaag tcgtgatgtt gttgttctaa gtaaccagtt tggaaatggt ttaatgaata
     1681 ttatctcccc aactagtggt gtattactgg caggattagc catcgcagag attaatttct
     1741 ctaaatggtt taaggttggc ttcaaaatat ttgccatctt aacccttgtt tctgctgttt
     1801 tgttatacat tgctacactc ttttagtctt aatcatccat taataggagg caatagttat
     1861 gattaaaata aatcaattag caggaatgaa tatccactat ttattttatt ctctcaaaga
     1921 atttttcgct tatcaacaga aaattggtat ttcttctgtt gaactttggg gaggtgctcc
     1981 gcattttttc atagatgctt tta
//
LOCUS       DAZKLM010000176         1983 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3764
            SAMEA115706913-rid22582563.denovo.184, whole genome shotgun
            sequence.
ACCESSION   DAZKLM010000176 DAZKLM010000000
VERSION     DAZKLM010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706913
            Sequence Read Archive: ERR13196647
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1983)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1983)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287823.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287823.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,004
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,849
            CDSs (with protein)               :: 2,849
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 41 of 96
            Pseudo Genes (incomplete)         :: 52 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 15 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1983
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706913-rid22582563.denovo.184"
                     /isolate="VRE3764"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..973)
                     /locus_tag="ACG842_002766"
     CDS             complement(<1..973)
                     /locus_tag="ACG842_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HFQ0249236.1"
                     /translation="MANENQLSEIQSKIEGRGAEKRTTINQLEKLNRISNRRLSVQGT
                     TTPLKLNWSIDRITIVGKLNDTIAYWTKNGVVFVDFEQLMRLNENQGYLKSVGINGWQ
                     LFDQYGEDIAYIEILKFHEGKGRIDFNPNKIGQFLASSMKGFIHDLFLDPHFSRADIA
                     CDIVDVPDDFITQYRIVDPISFKPIYGRSGKLETAYWGSRASERQVRLYNKKLEQERK
                     KVIVPKEIDTWWRLEMQLRRGKATDWHAMVQESLDSFASPHFLPIDIKPIDKIVIDGL
                     IAEPSNWSIIARHTKYKYRNLLKQESQNDELTNHLRETFKESADELKK"
     gene            complement(1554..1736)
                     /locus_tag="ACG842_002767"
                     /pseudo
     CDS             complement(1554..1736)
                     /locus_tag="ACG842_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313295.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          556 a          461 c          310 g          656 t
ORIGIN      
        1 ctttttttaa ttcgtctgca gattccttaa aagtctctcg taaatgattc gttaactcat
       61 cattttgact ttcttgcttc agcaaattcc gatatttata ttttgtatgt ctagcaatta
      121 tcgaccaatt agaaggctca gctatcaaac catcaataac tattttatca ataggtttta
      181 tatcaatcgg caaaaaatgc gggcttgcaa aactatctaa gctttcctga accatcgcat
      241 gccaatccgt tgctttgcca cgtcttagct gcatttctaa ccgccaccac gtatcgattt
      301 cttttggcac gattactttt tttcgttcct gctctagctt tttgttgtac agtcgcactt
      361 ggcgctcact ggcacgactg ccccagtacg ctgtttctag cttgccagaa cggccgtaga
      421 ttggcttaaa agaaatcggg tctacaatac gatactgagt aataaagtca tccggcacat
      481 ctacaatatc gcaggcaata tccgcccggg aaaaatgcgg atctaaaaat aggtcatgaa
      541 tgaatccttt catggaacta gctaaaaact ggcctatctt attcggatta aaatcaatac
      601 gaccttttcc ttcatgaaat ttaagtattt cgatatacgc aatatcttct ccatattgat
      661 cgaataattg ccagccgttt atcccaactg atttcaaata tccctgattt tcgttcaaac
      721 gcattaattg ctcgaaatcg acaaaaacaa ctccattttt cgtccaataa gctatggtat
      781 cattcagttt tccgacaatt gtaattcgat caatcgacca atttaatttt aggggggtgg
      841 tcgtaccttg cacacttaac cgcctgttag aaatacggtt aagtttttcg agctgattta
      901 tcgttgttct tttttctgcc cctcgacctt caatttttga ttgaatttca ctcaactgat
      961 tttcatttgc cattttcgtc ctccaaacga aaaaagagac ttttggaaag aatttccaga
     1021 gttcacgtaa ccctaaaaat tcatatccaa aaatctctag cttgaagaaa cctaaaaata
     1081 ttcgtataat gaacctgagt tacggagcta ggtgaagtag tttccgtaaa tagtcgagag
     1141 tgttagcgca ctgctcggct tttattttgc tcttttttct cgttttcttt gattatagca
     1201 ctcctcctaa aaaaataaaa agacctcagg gaatttcatt ccccgaaccc cttgagcctc
     1261 ctcctgccga aatggaaagg gcgcacccgc tgtttatttc attcagctat cggttttcac
     1321 aatctaacac gatcgttccg ctaccagtcg ccttacaggc tccattttcg ctcctctaac
     1381 gtgttttaac gattgtgccg ataactaaac gaaataaacg ctaaaaccgc tcagaacgca
     1441 aaatatgcca ttttagagct attgtagaaa atgtagtttg aaatgcaatt ttcttctttc
     1501 agctttttat ttcccacttg taaatctgct aattattttc taccagacaa accattgatt
     1561 ttttggctat cagttaactc acgattatta tgagaaatgc ttgtattctt tgttcccttt
     1621 ttgaacgaaa tactaaaccc taaggtgaac ccctccttca cgctgtcgct aataaaaaga
     1681 gtgtaaacct ttctgttact ctttgacaaa gagtaacgtt ttcctaattg tgacatggct
     1741 gtcacaattc caaacgctct ccgagggatt tcgctgtcac aagtgacaac gaaaaaagga
     1801 gccgaaagcg tgtcacgctc tcaactcctt ttacgcttta ttttgcttca tgcaaaatat
     1861 tcgcttatcg tgaatttacg tcactaaaca ttgacgcaaa tccactctca ctcatctgac
     1921 ttttcttatt cggtatcact tctctgtcac atccaacccc aaaagccacg tatccaattc
     1981 ttt
//
LOCUS       DAZKLM010000177         1916 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3764
            SAMEA115706913-rid22582563.denovo.185, whole genome shotgun
            sequence.
ACCESSION   DAZKLM010000177 DAZKLM010000000
VERSION     DAZKLM010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706913
            Sequence Read Archive: ERR13196647
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1916)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1916)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287823.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287823.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,004
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,849
            CDSs (with protein)               :: 2,849
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 41 of 96
            Pseudo Genes (incomplete)         :: 52 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 15 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1916
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706913-rid22582563.denovo.185"
                     /isolate="VRE3764"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..426
                     /locus_tag="ACG842_002768"
     CDS             <1..426
                     /locus_tag="ACG842_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFQ0249237.1"
                     /translation="IMNPKVEYIYEFEGGKGITNQYRPNKFETFKEFYSLANTVTDDI
                     NKDGMAALLLKGTTSVIFSIYSTSARTSKEKKDEAKQILSDPVIREAKQIAKTDSSAK
                     KITKLLYNMPAPLFLSAVKVGSFVEVKMPGLMAALKRVY"
     gene            445..1530
                     /locus_tag="ACG842_002769"
     CDS             445..1530
                     /locus_tag="ACG842_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HFQ0249238.1"
                     /translation="MKRILHFQGRMGLGGAESFMMNLYRKIDRTNYQFDFLIYEDYAD
                     VQDYHSEIERLGGRIFVVPNPKKNIFKYLIEVNKLLKKESFSIVHNQVYFGGGINLWL
                     AKKNGIRQRIAHSHATEDGKSQNIVMNVLRKFLTKLLLQNATDYLAVSQEAGESLFQN
                     HPFEIVHNGIDLELYSKNSEAKVNKRKELDISMSTFVVGNIGRLEKQKNQSYLLEIFL
                     EILKMNSDSYLILVGEGSLRNKLKAKAVELDIEKRVKFLGERNDIPELLSVMDVFVMP
                     SLYEGLPMVGLEAQAAKLKLVLADTISKDTKLTNNVDFVSLDETPQEWAKKVIKEPYA
                     NKITQELKSYDVSYTTEQMESIYSRDH"
     gene            1563..>1916
                     /locus_tag="ACG842_002770"
     CDS             1563..>1916
                     /locus_tag="ACG842_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-1,6-N-acetylglucosaminyltransferase"
                     /protein_id="HFQ0249239.1"
                     /translation="MKICYLILAYNDIGNLKRLVNRLNENAVFIIHIDKKTDIEPFIE
                     AFKSYDNVEFLCNKNRVKIYWGGFSIVQAEINLVKRALQNEKYLKYVLLSGADYPIKD
                     NEYIYNYFKKNSSVEF"
BASE COUNT          728 a          223 c          360 g          605 t
ORIGIN      
        1 atcatgaacc caaaagtaga atatatttat gaatttgaag gtgggaaggg gatcactaat
       61 caataccgac ctaacaagtt cgaaacattt aaagaattct attcattagc aaatactgta
      121 acagacgata ttaataaaga cggtatggcg gcgcttcttt taaaagggac aacttcagtt
      181 attttttcta tttatagtac ctctgcaaga acttcaaaag agaagaaaga cgaagcaaag
      241 cagatattga gtgatccagt gatccgagaa gccaagcaaa ttgctaagac ggattcttca
      301 gcaaaaaaaa taacaaagtt gctttacaat atgccagctc cactattttt atctgctgta
      361 aaagttggaa gcttcgttga agtgaaaatg ccagggttaa tggctgcttt gaagcgagta
      421 tattagttaa tggaggaaaa tataatgaaa agaattttgc attttcaggg acgaatgggt
      481 ttaggtggag cagagtcttt catgatgaat ttatatcgta aaattgatcg tacaaactat
      541 caatttgatt ttttgattta tgaagattat gcagatgttc aagactatca ttcagagatt
      601 gaaagattag gaggccgtat ttttgttgta cctaatccta agaaaaatat ttttaaatat
      661 ttaatagaag tgaataagtt actaaaaaaa gaatcttttt ctatagttca caatcaggtt
      721 tattttggcg ggggaattaa tttatggtta gccaaaaaga atggaatcag acagagaatt
      781 gctcatagcc atgcaactga agatggaaaa tctcaaaata ttgtaatgaa tgtattgcgt
      841 aaatttttaa ccaaattgtt gttacaaaat gcaacagact accttgccgt tagtcaagaa
      901 gctggtgaaa gtctgtttca aaaccacccc tttgagatag ttcataatgg tattgattta
      961 gagctttata gtaaaaattc agaagctaag gttaataaga gaaaagaatt ggatatctca
     1021 atgagtactt ttgttgtggg aaacattgga cgattagaga aacaaaaaaa tcaatcttat
     1081 ttgttagaga tttttttaga aatactaaaa atgaattctg attcttactt gattttagta
     1141 ggtgaagggt cacttagaaa taaactgaaa gccaaagcag tagaattgga tatagagaaa
     1201 agagtaaaat ttttaggaga aagaaacgat attcccgagt tattatctgt gatggatgtt
     1261 tttgtaatgc cttctcttta tgaaggtctg ccaatggttg gattagaagc acaagcagca
     1321 aaattaaaat tagtcttagc agatactatt tccaaagata caaagttaac aaataatgtg
     1381 gattttgtat cattagatga aacaccacaa gaatgggcaa aaaaagtaat aaaagagccg
     1441 tatgctaata aaataacaca agaactaaag agttatgatg tttcgtacac tactgaacaa
     1501 atggagagta tatatagtag agatcattaa aaatgttcaa gatattttag gagtgattat
     1561 ttttgaaaat atgttatttg atattggcat acaatgatat tggcaactta aagagattgg
     1621 tcaatcgatt gaacgaaaat gcagttttta ttattcatat agataaaaaa actgatatag
     1681 aaccatttat tgaagcattt aaatcatatg ataatgttga atttttatgt aataaaaacc
     1741 gtgttaaaat ttattggggt ggtttttcca ttgtacaagc agaaatcaat ttagtaaaga
     1801 gagcattaca aaatgagaaa tatttaaaat atgtattatt atctggtgct gattatccaa
     1861 tcaaagataa tgagtatatt tataattatt ttaaaaagaa tagttcagtg gagttt
//
LOCUS       DAZKLM010000178         1899 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3764
            SAMEA115706913-rid22582563.denovo.186, whole genome shotgun
            sequence.
ACCESSION   DAZKLM010000178 DAZKLM010000000
VERSION     DAZKLM010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706913
            Sequence Read Archive: ERR13196647
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1899)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1899)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287823.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287823.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,004
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,849
            CDSs (with protein)               :: 2,849
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 41 of 96
            Pseudo Genes (incomplete)         :: 52 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 15 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1899
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706913-rid22582563.denovo.186"
                     /isolate="VRE3764"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            130..570
                     /locus_tag="ACG842_002771"
     CDS             130..570
                     /locus_tag="ACG842_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005471259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFQ0249240.1"
                     /translation="MEPDLKTVNDYLVSVFNDILTIEESELKKSQFKDLSITEMHTIE
                     AIGMYKKKTTSEVAKELSITVGTLTTAINKLVKKGYVERIRSEDDRRVVKLGLTKKGK
                     LLFRVHQHFHREMVKNILDGMATEEQHALLDALKNLHDFLQDYK"
     gene            580..1542
                     /locus_tag="ACG842_002772"
     CDS             580..1542
                     /locus_tag="ACG842_002772"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HFQ0249241.1"
                     /translation="MEQFGMITATASVLPEKIITNNDLSKMMDTSDEWISSRTGIRER
                     RCVTNQETSDLCILVAEKLIKQKNLNPKDLDFILVATMSPDYTTPSVAAQVQGKLGAT
                     HAIAFDISAACSGFVYALSMAEKMIRCGSTKGLVIGGETLSKLIDWSDRTTAVLFGDG
                     AGGILLEANPQKKLLKEKLAADGTRSSSLTAGYQQNKNPFYSEDSEGSYYLQMTGRDI
                     FDFAVRDVANNIQEVMKDKPVDYLLLHQANLRIIEKIARKVKMPQEKFLTNMDKYGNT
                     SAASIPILLDEAVSSGKITLGKQQKVIFTGYGGGLTWGSILMEL"
     gene            1595..1819
                     /locus_tag="ACG842_002773"
     CDS             1595..1819
                     /locus_tag="ACG842_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000035 - acyl binding [Evidence IEA];
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFQ0249242.1"
                     /translation="MVFEKIQEIIAEELGKETEEIKLTTDIQEDLEADSLDLFQIINE
                     IEDEFDVKIETEDGIKTVQDLVTYVEKQTA"
BASE COUNT          686 a          304 c          408 g          501 t
ORIGIN      
        1 tgagtaaaat cttaaaaaaa gctcatatag taaaaaaatg atcttttaag gttgcatttt
       61 gaaaaaagtt aagatatagt ttgaacatca aaagatttga taatcaaact aattagtgga
      121 cggtggaaca tggaaccaga tttaaaaaca gtaaatgatt atcttgttag tgtcttcaat
      181 gatattttga caatcgaaga gtctgagctg aaaaaatctc aattcaaaga tctgtcaatc
      241 acagagatgc acacgatcga ggcaatcggg atgtacaaga aaaaaacaac ttcagaagta
      301 gccaaagaac tatcaattac tgttgggacc ttgaccactg ctattaacaa gttagtaaaa
      361 aaaggctatg tcgaacgtat ccgcagtgaa gatgaccgcc gcgttgtaaa gttgggacta
      421 acgaaaaaag gaaagttact atttcgggtc catcagcatt ttcatcgtga aatggtcaag
      481 aacatcttag acggaatggc tacggaggaa cagcatgcct tgctggatgc actgaaaaac
      541 ttgcacgact tcttgcaaga ctataagtga ggatatttaa tggaacaatt tggaatgatt
      601 actgcaacag ccagcgtcct tcctgaaaag attattacta ataatgatct ttccaagatg
      661 atggatacct ctgatgaatg gatttcgtca aggacgggta ttcgtgaacg aagatgtgta
      721 acaaatcagg agacttctga tttatgcatt ttagtggcgg aaaaattgat aaaacagaaa
      781 aatctaaacc ctaaagattt ggattttatt ttagtagcaa caatgtcgcc cgattacacg
      841 acaccatctg tagcagcaca agtacaaggg aaattgggag caactcatgc tatcgctttt
      901 gatatcagtg ctgcgtgctc aggatttgtt tatgcgttaa gcatggctga aaaaatgatc
      961 cgctgcggct caactaaggg actggtgatt ggtggtgaaa cactatctaa gctaatcgat
     1021 tggtctgatc gtacgactgc tgtgttgttt ggcgatggag caggtgggat tctcttagaa
     1081 gcgaatccac agaaaaaatt gctgaaagaa aaattagcag ctgacggtac cagaagtagc
     1141 tcattgaccg ctggctacca gcaaaataaa aatccgttct attcagaaga ttcagaaggt
     1201 tcgtattatt tgcaaatgac tggcagagat atttttgatt ttgccgtacg agacgttgca
     1261 aacaatatcc aagaagtcat gaaagacaaa ccagttgatt atctgctttt gcatcaggca
     1321 aacctcagaa ttattgagaa aattgcccga aaagtaaaga tgccgcaaga aaagttcctg
     1381 accaatatgg ataaatatgg gaatacatct gctgcaagca tccctatctt attggatgaa
     1441 gcagtttcta gtggaaagat caccttagga aaacagcaga aagtaatatt tacaggctat
     1501 ggcggaggtt taacatgggg aagtattctc atggagttgt agactgttaa attataaaaa
     1561 taaaaagcaa acaatatcgg aggaatatat tattatggta ttcgaaaaaa ttcaagaaat
     1621 catcgcagaa gaattaggaa aagaaactga agaaatcaaa ttgactactg acatccaaga
     1681 agacttagaa gctgacagct tggatttatt ccaaatcatc aatgaaattg aagatgaatt
     1741 tgacgtgaaa atcgaaacag aagacggaat caaaacggtt caagatttag tgacttacgt
     1801 agaaaaacaa actgcataat cattaaaatt aggttggggg ctgggacatt tcctgaaata
     1861 ggaatgtctc ggcctttttg aataggaaga atcaaacat
//
LOCUS       DAZKLM010000179         1863 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3764
            SAMEA115706913-rid22582563.denovo.187, whole genome shotgun
            sequence.
ACCESSION   DAZKLM010000179 DAZKLM010000000
VERSION     DAZKLM010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706913
            Sequence Read Archive: ERR13196647
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1863)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1863)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287823.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287823.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,004
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,849
            CDSs (with protein)               :: 2,849
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 41 of 96
            Pseudo Genes (incomplete)         :: 52 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 15 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1863
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706913-rid22582563.denovo.187"
                     /isolate="VRE3764"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(35..736)
                     /locus_tag="ACG842_002774"
     CDS             complement(35..736)
                     /locus_tag="ACG842_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HFQ0249243.1"
                     /translation="MSVILISGTIGAGKSSLTDMLAKEIDSKPFYENVEDNEVLPLFY
                     SNPEQYAFLLQIFFLNKRFLAMKNALVNDDNVLDRSIYEDSLLFHLNADLGRVTDIEV
                     QQYDSLLDTMLNELDDVAPKKRPDLMVHIKVSLDTMLERIKKRGRDYEQLESDETLYT
                     YYETLNTRYNQWYEDFDVCPKIQVDGDKFDFVENPEDAKKVIRQIQEKLAELEGKPAG
                     KYFTDNQHAKGQELG"
     gene            complement(752..1192)
                     /locus_tag="ACG842_002775"
     CDS             complement(752..1192)
                     /locus_tag="ACG842_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside 2-deoxyribosyltransferase"
                     /protein_id="HFQ0249244.1"
                     /translation="MSQIYLAGPFFSEEQIDRVSRIEKALEENKTVTSFYSPRHHQES
                     NYELFSAGWAQEVYEKDMEELTNAEFVVAILDFEHQTIDPGTAYELGVATMLKKPMII
                     VQEETVPTNLMITQSLHTYLKSDQAVREYDFETLPVETYVGEYL"
     gene            complement(1192..1767)
                     /locus_tag="ACG842_002776"
     CDS             complement(1192..1767)
                     /locus_tag="ACG842_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009691 - cytokinin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HFQ0249245.1"
                     /translation="MKLTVFCGSRFGNKESYKFIAQTLGKYMAQEEIELVYGGSYSGI
                     MGVISKTVLENNGKVTGIYPNGLFETELPGKDVTTFIPTETIDERKVLLFEKGDAVLV
                     FPGGLGTLEEFSQLLSWIAIGLTPDKPIGILNIGGYYSGLQQLLETFAKEDFMDKKWL
                     DQVIFSNNPLELVNELRAVSIKNQLLLKEAK"
BASE COUNT          522 a          400 c          290 g          651 t
ORIGIN      
        1 taatttttct ttcccgtcgt ttggtttttt atttttagcc taattcttga ccttttgcat
       61 gctggttatc cgtaaaatat tttccagcag gctttccttc caattcagct aatttttctt
      121 ggatctgacg gatcaccttt tttgcatctt ctggattttc tacaaaatcg aatttatccc
      181 catccacttg tattttagga catacatcaa aatcttcata ccattgatta tagcgggtgt
      241 tcaacgtttc ataataggta tataacgtct catcgctttc tagctgttca tagtcacgcc
      301 cacgtttttt gatacgttct agcatcgtgt ccaaagaaac tttgatatgg accatcaaat
      361 caggccgttt ttttggagca acatcatcta actcgtttag catcgtatct aataagctat
      421 cgtactgctg tacttcgata tccgttaccc gacctaaatc agcgtttaga tggaacaaca
      481 ggctatcttc atagatggat cggtctagca cattgtcatc gttcactaaa gcatttttca
      541 ttgctaaaaa tcgcttgttc aaaaagaaga tttgcaataa aaaagcgtac tgctcaggat
      601 ttgaataaaa gagcggcaat acttcgttat cttcgacatt ttcataaaat ggtttagaat
      661 caatctcttt tgcaagcata tccgtcaaac tgctcttacc tgcaccaatc gttcctgaaa
      721 tcaaaatcac tgacatacaa cccttcctct tctataagta ctcgcctacg taagtttcaa
      781 caggcaatgt ttcaaaatca tattcacgta cagcttggtc tgattttaaa tacgtatgga
      841 gactttgtgt aatcatcaag ttcgtaggta ccgtctcttc ttgtacaatg atcatcggtt
      901 ttttcaacat agttgccaca cctaattcgt aagccgttcc tggatcgatt gtttgatgct
      961 caaagtcaag gatagcaaca acaaattctg catttgtcag ttcttccata tctttttcgt
     1021 atacttcctg cgcccatcca gcgctgaata gttcataatt gctttcttgg tgatgacgtg
     1081 gagagtagaa acttgtgact gttttgtttt cctccaacgc tttttcgata cgacttactc
     1141 gatcgatttg ctcctctgaa aaaaatggtc ctgctaaata tatttgactc attattttgc
     1201 ctccttcaat aataactgat ttttaataga tactgctcgt aattcattga ccagttccaa
     1261 tggattgttg gaaaagatta cttgatccaa ccatttttta tccataaaat cctcttttgc
     1321 aaaagtttct aataattgtt gcaagccgct gtaataaccg ccaatattca atattccgat
     1381 tggcttgtcg ggcgtcagac cgattgctat ccaagaaaga agttgtgaga attcctccaa
     1441 tgtacctaga cctccaggga aaacgaggac agcatctccc ttttcaaata ataatacctt
     1501 tctttcatcg atcgtttctg taggaataaa tgtagtaaca tcttttccag gcaattcagt
     1561 ttcaaaaagc ccattcggat agatacctgt gacttttccg ttattttcca gtactgtttt
     1621 actaatcact cccataatcc cggagtatga accaccgtaa accagttcga tttcttcttg
     1681 tgccatgtat tttcctaacg tttgcgcaat aaatttgtaa ctttctttat taccaaaacg
     1741 ggaaccgcaa aaaacagtaa gtttcataaa aaaacactcc ctaactcaaa agtctatctt
     1801 cattctacac gtgttttttt aaaataaaag actagatttt gtagtttttt agtggtatat
     1861 agg
//
LOCUS       DAZKLM010000180         1818 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3764
            SAMEA115706913-rid22582563.denovo.188, whole genome shotgun
            sequence.
ACCESSION   DAZKLM010000180 DAZKLM010000000
VERSION     DAZKLM010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706913
            Sequence Read Archive: ERR13196647
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1818)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1818)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287823.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287823.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,004
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,849
            CDSs (with protein)               :: 2,849
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 41 of 96
            Pseudo Genes (incomplete)         :: 52 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 15 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1818
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706913-rid22582563.denovo.188"
                     /isolate="VRE3764"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            275..1753
                     /locus_tag="ACG842_002777"
     CDS             275..1753
                     /locus_tag="ACG842_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0249246.1"
                     /translation="MKLDYLIEEEDREKLLLARYLEFEKKNYVVLPKLYETLGLSKFK
                     TRNYIIELNKEFTEFKTKPEIQISEEAEIHVERLNLTDIKNLRRIYFQRSKIARLLTE
                     IVDSKVTIEKFAENHFLSISRAYVKRRELVSFLEQHQIKLKKNILVGEEINIRNVLFG
                     VYFGVFNGFSMPFRESVMNEAKSIVQYFEYLFNLKLSATKRIKFNLLTTISLLRAYGN
                     QPIGKRFFSDEYFKSSEGMKIIKDLSHISFLHIPKEQIIDEISYIMLFLITEEEFELD
                     GAFGLAEAHFHDIHMISQKMSVELIDQLPFSIEISEEERLYIRKELRMGMMHVNRKNW
                     IFDFEMTSFTSKKQLQFFFETYPIFSTAIQKVIQENKRYFLGKDERFMIRLFYDYLFL
                     LVGICPVSYFEAPIHVCIDFSQGSAYTDYIISQVKGFKNYNIVIESRITSRTNIYLSD
                     CLLEKFNKEQIIWKNPPTPDDWEYFGNTIIREKNRHLTEKIS"
BASE COUNT          661 a          253 c          324 g          580 t
ORIGIN      
        1 taaaagagca ggacggtggc cgtcctgctc ttttttgtgg ttgtttacgc aaatggcgca
       61 aatcttgtac attttaacac aataaaacaa aaaatataga atatttcctc ataaattttt
      121 ccattttttg caatattctc aaaaaaaaga taaaaaatga gaataactga tgaaaaaaca
      181 agcataatcc atcataaaat cagtataggt gaaaagatga actttcatct gcatatcaag
      241 taattggtat gaggcatttt ttgaggtgta ggatatgaag ttggattacc ttatagaaga
      301 ggaagataga gaaaaactgc tgctagctag atatttagaa tttgagaaaa agaattatgt
      361 agtgctgcct aagttatatg aaacattagg tttgtctaaa tttaaaacaa gaaattatat
      421 catagaatta aataaagaat ttactgaatt caaaacaaag ccagagatcc aaatttcaga
      481 agaagcagag atccatgtcg aacgtctgaa tttgacggat atcaaaaatt taagaagaat
      541 ctactttcaa cgatcaaaaa ttgctcgact gttaacggag atcgtggatt caaaagtaac
      601 gatcgaaaaa tttgcagaga atcatttttt aagcatatca agagcctatg taaaaagaag
      661 agaattagtc tcttttcttg aacagcatca aataaagtta aagaaaaaca tattagttgg
      721 ggaagaaata aacatacgga acgtattatt tggggtatat ttcggggtct ttaatggttt
      781 ttctatgcca tttagagaaa gcgtcatgaa tgaagcaaag agtatcgtgc aatattttga
      841 atatctgttc aatttaaaac tgtctgcaac aaaacgaatc aaattcaatt tgctaaccac
      901 tattagtttg ttgagggcct atgggaatca accaatcgga aaacgtttct tttcagatga
      961 atactttaag agctcagaag gaatgaagat catcaaggat ttatcacata tttcttttct
     1021 tcatatccct aaagagcaga tcattgatga gatttcttat atcatgttgt ttttgatcac
     1081 ggaagaagaa tttgaattag atggcgcatt tggacttgcg gaagctcatt ttcatgatat
     1141 ccacatgatt agtcaaaaaa tgtcagtgga attgattgat caattaccat tttcgattga
     1201 aatctctgaa gaagaaagac tatatatacg aaaagaactt cgtatgggta tgatgcatgt
     1261 caatcgaaaa aattggatat tcgactttga gatgactagc tttacgtcta aaaagcagct
     1321 acagtttttc ttcgagacgt atccaatatt cagtacagca atccaaaagg tcatacaaga
     1381 gaataaacgc tatttcttgg gaaaagatga acgtttcatg attcgcttat tttatgatta
     1441 tctcttttta ttggtcggta tttgtccggt atcatatttt gaagcaccga ttcatgtctg
     1501 tattgatttt tcgcaaggat ctgcttatac cgattacatt atttctcaag tgaaaggatt
     1561 taaaaattac aatattgtga ttgagagtcg aatcacttct cgtacgaata tttatttatc
     1621 tgattgttta cttgaaaaat ttaataaaga acagataatt tggaaaaatc caccgactcc
     1681 agatgactgg gaatattttg gaaataccat catcagagaa aaaaatagac atttaactga
     1741 aaaaatcagc tagtatttat ggatggcagc tttgtctcac atatttatta gctgatttat
     1801 gtttttttca aagaaaag
//
LOCUS       DAZKLM010000181         1799 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3764
            SAMEA115706913-rid22582563.denovo.189, whole genome shotgun
            sequence.
ACCESSION   DAZKLM010000181 DAZKLM010000000
VERSION     DAZKLM010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706913
            Sequence Read Archive: ERR13196647
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1799)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1799)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287823.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287823.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,004
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,849
            CDSs (with protein)               :: 2,849
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 41 of 96
            Pseudo Genes (incomplete)         :: 52 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 15 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1799
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706913-rid22582563.denovo.189"
                     /isolate="VRE3764"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            157..894
                     /gene="erm(B)"
                     /locus_tag="ACG842_002778"
     CDS             157..894
                     /gene="erm(B)"
                     /locus_tag="ACG842_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011100845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HFQ0249247.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESRASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTITYEQVLSIFNSYLLFNGRK"
     gene            1064..1324
                     /locus_tag="ACG842_002779"
                     /pseudo
     CDS             1064..1324
                     /locus_tag="ACG842_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108743.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase DNA-binding C4 zinc finger
                     domain-containing protein"
     gene            1458..1697
                     /locus_tag="ACG842_002780"
     CDS             1458..1697
                     /locus_tag="ACG842_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002425012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
                     /protein_id="HFQ0249248.1"
                     /translation="MIVGNLGAQKEKRNDTPISAKKDIMGDKTVRVRADLHHIIKIET
                     AKNGGNVKEVMEIRLRSKLKSVLIVHYLKILYNRN"
BASE COUNT          704 a          272 c          312 g          511 t
ORIGIN      
        1 agactaaaaa cagtgattac gcagataaat aaatacgtta gattaattcc taccagtgac
       61 taatcttatg actttttaaa cagataacta aaattacaaa caaatcgttt aacttctgta
      121 tttgtttata gatgtaatca cttcaggaga gattacatga acaaaaatat aaaatattct
      181 caaaactttt taacgagtga aaaagtactc aaccaaataa taaaacaatt gaatttaaaa
      241 gaaaccgata ccgtttacga aattggaaca ggtaaagggc atttaacgac gaaactggct
      301 aaaataagta aacaggtaac gtctattgaa ttagacagtc atctattcaa cttatcgtca
      361 gaaaaattaa aactgaatac tcgtgtcact ttaattcacc aagatattct acagtttcaa
      421 ttccctaaca aacagaggta taaaattgtt gggaatattc cttaccattt aagcacacaa
      481 attattaaaa aagtggtttt tgaaagccgt gcgtctgaca tctatctgat tgttgaagaa
      541 ggattctaca agcgtacctt ggatattcac cgaacactag ggttgctctt gcacactcaa
      601 gtctcgattc agcaattgct taagctgcca gcggaatgct ttcatcctaa accaaaagta
      661 aacagtgtct taataaaact tacccgccat accacagatg ttccagataa atattggaag
      721 ctatatacgt actttgtttc aaaatgggtc aatcgagaat atcgtcaact gtttactaaa
      781 aatcagtttc atcaagcaat gaaacacgcc aaagtaaaca atttaagtac cattacttat
      841 gagcaagtat tgtctatttt taatagttat ctattattta acgggaggaa ataattctat
      901 gagtcgcttt tttaaatttg gaaagttaca cgttactaaa gggaatggag ataaattatt
      961 agatatacta ctgacagctt ccaagaagct aaagaggtcc ctagcgccta cggggaattt
     1021 gtatcgataa ggggtacaaa ttcccactaa gcgctcggga ccccttgtag gaaaatgtcc
     1081 taagtgtggc aacaatattg tattaaaaaa atcgttttat ggttgttcaa attatcctga
     1141 atgtaagttt actttagctg aacattttag aaagaaaaaa ctaaccaaaa cgaatgtaaa
     1201 agaattactg gagggaaaag aaaccctggt aaaaggaatc aaaaacaaag agaaaaagcc
     1261 ctacaatgcc gttgtaaaaa ttggggaaaa gggatatatt gattttatat ctttctcaaa
     1321 ataaacataa aagcccttta aagagggctt ttatatatta atcacaaatc acttatcaca
     1381 aatcacttat cacaaatcac aagtgatttg tgattgttga tgataaaata agaataagaa
     1441 gaaatagaaa gaagtgagtg attgtgggaa atttaggcgc acaaaaagaa aaacgaaatg
     1501 atacaccaat cagtgcaaaa aaagatataa tgggagataa gacggttcgt gttcgtgctg
     1561 acttgcacca tatcataaaa atcgaaacag caaagaatgg cggaaacgta aaagaagtta
     1621 tggaaataag acttagaagc aaacttaaga gtgtgttgat agtgcattat cttaaaattt
     1681 tgtataatag gaattgaagt taaattagat gctaaaaatt tgtaattaag aaggagggat
     1741 tcgtcatgtt ggtattccaa atgcgtaatg tagataaaac atctactgtt ttgaaacag
//
LOCUS       DAZKLM010000182         1766 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3764
            SAMEA115706913-rid22582563.denovo.190, whole genome shotgun
            sequence.
ACCESSION   DAZKLM010000182 DAZKLM010000000
VERSION     DAZKLM010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706913
            Sequence Read Archive: ERR13196647
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1766)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1766)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287823.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287823.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,004
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,849
            CDSs (with protein)               :: 2,849
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 41 of 96
            Pseudo Genes (incomplete)         :: 52 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 15 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1766
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706913-rid22582563.denovo.190"
                     /isolate="VRE3764"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            73..234
                     /locus_tag="ACG842_002781"
     CDS             73..234
                     /locus_tag="ACG842_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HFQ0249249.1"
                     /translation="MQDKDKCINCGKCKKVCQMNKDSVENCNHLECIRCGRCKNACPV
                     DAISYGVRK"
     gene            253..1530
                     /locus_tag="ACG842_002782"
     CDS             253..1530
                     /locus_tag="ACG842_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0249250.1"
                     /translation="MIKNAIAYITRKKNRTLIIFIILTIVLSCLYSCLTIMKSSNEIE
                     KALYESSNSSISITKKDGKYFNVNQFKDIEKLKEIEEKIIQYDGLAKLKDAKVVSGEQ
                     RINREDLSDEFKNVVSLEATNNTKRNILFSSGVFTIKEGKNIEENDKNSIIVHEEFAK
                     QNNLKLGDEVDLELLDIEKSGKIKSHKFKIIGIFSGKKQETYTGLSSDFSENMVFVDY
                     STSQEILNNSENNEIANKILMYSGSAESTDLALNKLKELKIDESKYFVEKDSNAFEES
                     LESVSGIKYIIKVMTYSIMLGGMVVLSLILILWLRERIYEIGIFLSIGTSKIQIIMQF
                     IFELIFISIPSIISSLFLGNVLLKVIVDGFINSEDSMISGGSLINNSSFMSNITTLGQ
                     SYLILISIIVLSVVFASSLMLIKKPKEILSKIS"
     gene            1540..>1766
                     /locus_tag="ACG842_002783"
     CDS             1540..>1766
                     /locus_tag="ACG842_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004612033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFQ0249251.1"
                     /translation="MDILEIKNVAYSYANSKEKVLSGVNQKFELGKFYAIVGKSGTGK
                     STLLSLLAGLDKPQTGKILFKNEDIQNKGYS"
BASE COUNT          723 a          163 c          298 g          582 t
ORIGIN      
        1 catgaaaaat cctccagtgt gttttttatt attctacaca ccttattttt tctgtctagt
       61 ctagtgtagc cgattcagga taaggataag tgtattaatt gtggaaaatg taaaaaagtt
      121 tgccaaatga ataaagattc agttgagaat tgcaatcatc tggagtgtat cagatgtgga
      181 cgatgtaaaa atgcttgtcc agtggatgca attagttatg gagtaagaaa atagagagga
      241 gtagttagga aagtgataaa aaatgctata gcatatatta caagaaagaa aaatagaact
      301 ttaataattt ttatcatatt aactatagtt ctttcttgct tgtattcttg tttaacaata
      361 atgaaatcaa gcaatgaaat agaaaaggct ttatatgaaa gttctaattc ttcaatatca
      421 ataacaaaaa aagatggtaa atattttaat gttaatcaat ttaaagatat tgaaaaatta
      481 aaagaaattg aagaaaaaat aattcaatat gatggattag caaaactaaa agatgctaaa
      541 gtagttagcg gagaacaaag aataaataga gaagatttat ctgacgaatt taagaatgtt
      601 gtttcactcg aagctacaaa taatactaaa agaaatattt tatttagtag tggagtattt
      661 acaattaaag aaggtaaaaa tatagaagaa aatgataaga attcaattat tgttcatgaa
      721 gaatttgcta aacaaaacaa tctaaaatta ggtgatgaag ttgatcttga attactagat
      781 attgaaaaaa gtggaaaaat aaaaagtcat aaatttaaaa ttatagggat cttttctggt
      841 aaaaaacagg aaacatatac aggattatct tctgatttta gtgaaaatat ggtgtttgta
      901 gattattcaa caagccaaga gatattaaat aattcagaaa ataatgaaat tgcaaataaa
      961 attttaatgt attctggtag tgcagaatct acagacttag ccttaaacaa attgaaagag
     1021 cttaaaattg atgagtcaaa gtattttgtt gaaaaagatt ctaatgcgtt tgaagagtct
     1081 ttagagtcag tgagtggaat aaaatatata ataaaagtaa tgacttattc tattatgtta
     1141 ggtgggatgg ttgttctttc attaatcttg attctatggt taagggaaag aatttatgaa
     1201 ataggtatat tcttatctat tggaacatct aagatacaaa ttataatgca atttatattc
     1261 gagttaatat tcatatcgat accaagtata atatcttcct tatttttagg aaatgtatta
     1321 ctaaaagtaa ttgtagatgg attcattaac tcagaggatt caatgatttc cggtggaagt
     1381 ttaataaata atagtagttt tatgtcaaat ataacaacac ttggacaaag ttatttaata
     1441 ttaataagta ttattgtttt atcagttgta tttgcttctt cattaatgtt aatcaagaag
     1501 cctaaagaaa tcttatcaaa aataagttag gagaaaataa tggatatatt agaaataaag
     1561 aatgtagcat atagttatgc aaattctaaa gaaaaagttt tgtcaggagt aaatcaaaaa
     1621 tttgaacttg ggaagtttta tgcgatagta ggaaagtcag gaacaggaaa atctacactt
     1681 ctttccttac ttgctggact tgataaacct caaacaggaa aaatattgtt taagaatgaa
     1741 gatatacaaa ataaaggata tagtaa
//
LOCUS       DAZKLM010000183         1731 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3764
            SAMEA115706913-rid22582563.denovo.191, whole genome shotgun
            sequence.
ACCESSION   DAZKLM010000183 DAZKLM010000000
VERSION     DAZKLM010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706913
            Sequence Read Archive: ERR13196647
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1731)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1731)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287823.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287823.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,004
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,849
            CDSs (with protein)               :: 2,849
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 41 of 96
            Pseudo Genes (incomplete)         :: 52 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 15 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1731
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706913-rid22582563.denovo.191"
                     /isolate="VRE3764"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..156
                     /locus_tag="ACG842_002784"
     CDS             <1..156
                     /locus_tag="ACG842_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HFQ0249252.1"
                     /translation="GTYGHTGVIRGLANGRMQTYEQNTELGMICGKLDRQYYSASAIS
                     SIVIPPK"
     gene            169..1077
                     /locus_tag="ACG842_002785"
     CDS             169..1077
                     /locus_tag="ACG842_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate upper protein"
                     /protein_id="HFQ0249253.1"
                     /translation="MAKWNVILSTTEPYNYVGMIQVRQGNKNTEVMEATIVENGLPYD
                     LSECKVYFESVVGGKYPVQLETKIVDAKKGKINYIFDKYSMQCLHRQTANFIIFKGED
                     LIGTTQDFSYFVINAVSKTEGEMGSYWQSIEDLIADMTDFINENKGDFTDWMNERKEE
                     FERWRQEQEHSFQDWREGQESDYLAWFESIKDILKTIDPGGVMLAELTDARVDIQGVR
                     HASISERLLADMDYLYQKLRATLFTIEYGEIEVTDILQDDLFSDNHEVEKVGTVAFPI
                     EEGALIIATVDDPKQNVFTLEKVGVI"
     gene            1077..1697
                     /locus_tag="ACG842_002786"
     CDS             1077..1697
                     /locus_tag="ACG842_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0249254.1"
                     /translation="MAKTKRMMETDEKTGVQRQFFPITHASAVLGLEEIIAGEATVLS
                     VNGKIGAVVITKEDLGLENVLTELPYASEEDDGIITAEMYQKILNSGEGDYVLPVATI
                     DRLGGIKVGELLTIDETGKVSAVRQSDVNFSLELKEKLDSLKNYTAGENITIDEDGTI
                     SSTGGSGTGGVNQSYVDQKFQEAVNQAENYTNERIPNFTFEKIGEV"
BASE COUNT          613 a          251 c          395 g          472 t
ORIGIN      
        1 ggaacttatg gacatactgg cgttatccga ggcttagcta atggtcgtat gcaaacttat
       61 gaacaaaaca cagagttagg tatgatttgt ggaaagttag atcgccagta ttatagtgca
      121 agtgcaattt cttccattgt cataccacca aaatagggag gtgatttaat ggcaaagtgg
      181 aatgtcatat taagtacaac agaaccctat aattacgtgg ggatgattca agttcgacaa
      241 ggcaataaga atacagaggt tatggaagcg actatagttg aaaatggtct tccctacgat
      301 ttatcagaat gtaaggtata ttttgaatca gttgtaggtg ggaaatatcc agtccaatta
      361 gaaacaaaaa ttgtggatgc taaaaaaggg aaaattaact atatttttga taaatattcc
      421 atgcagtgtt tacatcgaca aacagccaat ttcattatat ttaaaggaga agacttgatt
      481 ggaacaactc aagacttctc ttattttgtc attaatgctg tttcaaaaac agaaggagaa
      541 atgggttctt attggcaatc aatcgaagat ttaattgcgg atatgacgga ctttattaat
      601 gaaaataaag gcgattttac ggactggatg aatgaaagga aagaagaatt tgaacgttgg
      661 agacaagaac aggaacacag tttccaagat tggagagaag gacaagaatc agactatttg
      721 gcatggtttg aatcaatcaa ggatatttta aagacgattg atccaggtgg agtcatgtta
      781 gccgaactaa cggatgcgcg agtagatatt caaggagttc gtcatgcgtc tatttcagag
      841 cgtttgctgg cagatatgga ctatttatat cagaaattgc gagcaacgct tttcacgatt
      901 gaatacgggg agattgaagt gactgatatt ttgcaggatg atctcttttc agataatcat
      961 gaagtcgaaa aagttggaac tgtagcattc ccgattgaag aaggagcctt gatcatcgca
     1021 accgttgatg atccaaagca aaatgtgttc actcttgaga aagttggggt gatctaatgg
     1081 ctaaaactaa acgaatgatg gaaacggatg aaaaaacggg tgtacaacgc caattctttc
     1141 caatcacaca tgcttccgca gttcttgggt tagaagaaat aatagcagga gaagcaacag
     1201 ttttatctgt taatggaaaa attggagccg tcgtcattac taaagaagat ttgggattag
     1261 agaatgttct cacagaatta ccctatgcaa gtgaagaaga tgacggcatt atcacggcag
     1321 aaatgtatca aaaaatttta aacagtggag aaggtgacta cgtgttgcca gttgccacta
     1381 tcgaccgttt gggtggcata aaagttggtg aattattgac gattgatgag acagggaaag
     1441 tctctgcagt cagacaatct gatgtcaatt tttcgctgga gttaaaagaa aaactcgatt
     1501 cactgaaaaa ttatactgct ggagaaaaca tcactattga tgaagatgga acaattagtt
     1561 ctacaggtgg tagtgggact ggtggtgtca atcagtcata tgtagatcaa aaattccaag
     1621 aagctgtaaa tcaagcagaa aattacacaa acgaaagaat tccaaatttt acttttgaaa
     1681 aaattgggga ggtatagaga atgacagata tcgtaaaaat aaaacaaagc a
//
LOCUS       DAZKLM010000184         1728 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3764
            SAMEA115706913-rid22582563.denovo.192, whole genome shotgun
            sequence.
ACCESSION   DAZKLM010000184 DAZKLM010000000
VERSION     DAZKLM010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706913
            Sequence Read Archive: ERR13196647
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1728)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1728)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287823.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287823.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,004
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,849
            CDSs (with protein)               :: 2,849
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 41 of 96
            Pseudo Genes (incomplete)         :: 52 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 15 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1728
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706913-rid22582563.denovo.192"
                     /isolate="VRE3764"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..156
                     /locus_tag="ACG842_002787"
     CDS             <1..156
                     /locus_tag="ACG842_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HFQ0249255.1"
                     /translation="GTYGHTGVIRGLANGRMQTYEQNTELGMICGKLDRQYYSASAIS
                     SIVIPPK"
     gene            169..1077
                     /locus_tag="ACG842_002788"
     CDS             169..1077
                     /locus_tag="ACG842_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate upper protein"
                     /protein_id="HFQ0249256.1"
                     /translation="MAKWNVILSTTEPYNYVGMIQVRQGNKNTEVMEATIVENGLPYD
                     LSECKVYFESVVGGKYPVQLETRIVDAKKGKIKYIFDKYSMQCLHRQTANFIIFKGED
                     LIGTTQDFSYFVINAVSKTEGEMGSYWQSIEDLIADMTDFINENKGDFTDWMNERKEE
                     FDRWREEQENSFQDWREGQESDYLSWFESIKDILKSIDPGGVMLAELMDARVDLQGVR
                     HESISERFLSDLNYLYQKMKAALFTIEYGEIEVTDILQDDLFSDNHEVEKIETVEFPI
                     EEGALIIATVDDPKQNVFTLEKVGVI"
     gene            1077..1694
                     /locus_tag="ACG842_002789"
     CDS             1077..1694
                     /locus_tag="ACG842_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0249257.1"
                     /translation="MVKVKRMMETDESGVKRQFMPITHVSAVLGLEKMISGQSKVLSV
                     NGKYGAVILTKADLGLENAITELPYASETSDGILTAEMFQKIVNGEGGTYILPIATPE
                     QLGGIKVGELLEVTEEGVLSATKQTDFNFSEELKGKLESLKILKAGANISIAEDGTIS
                     STGGSGTGGVNQSYVDQKFQEAVNQAENYTNERIPNFTFEKIGEV"
BASE COUNT          629 a          231 c          368 g          500 t
ORIGIN      
        1 ggaacttatg gacatactgg cgttatccga ggcttagcta atggtcgtat gcaaacttat
       61 gaacaaaaca cagagttagg tatgatttgt ggaaagttag atcgccagta ttatagtgca
      121 agtgcaattt cttccattgt cataccacca aaatagggag gtgatttaat ggcaaagtgg
      181 aatgtcatat taagtacaac agaaccctat aattacgtgg ggatgattca agttcgacaa
      241 ggcaataaaa acactgaagt tatggaagct acaattgtcg aaaatggtct tccatatgat
      301 ttatcagaat gtaaggtata ttttgaatca gttgttggcg gaaaatatcc agtccaatta
      361 gaaacaagaa ttgtggatgc taaaaaaggg aaaatcaaat atatttttga taaatattcc
      421 atgcagtgtt tgcatcgaca aacagccaat ttcattatat ttaaaggaga agacttgatt
      481 ggaacaactc aagacttctc ctattttgtc atcaatgctg tttcaaaaac agaaggagaa
      541 atgggttctt attggcaatc aattgaagat ttaattgctg atatgacaga ttttattaat
      601 gaaaataaag gcgattttac ggactggatg aatgaaagga aagaagaatt tgatcgctgg
      661 agagaagaac aggagaatag tttccaagat tggagagaag gacaagaatc agattatttg
      721 tcatggtttg aatcaatcaa ggatattttg aagtctatcg atccaggtgg agtaatgtta
      781 gccgaattaa tggatgcacg tgtagatctt caaggtgtac gccatgaatc gatttctgaa
      841 cgtttcctat ccgatctaaa ttatttgtat caaaaaatga aagcagcact tttcacgatt
      901 gaatacggtg aaattgaagt gactgatatt cttcaagacg atctcttttc agataatcac
      961 gaagtcgaaa aaattgaaac agtagaattt ccaattgaag aaggagcttt gattattgca
     1021 actgttgatg atccaaaaca gaatgttttc actcttgaga aagttggggt gatttgatgg
     1081 ttaaagtaaa gagaatgatg gagactgatg aatctggtgt taaacgtcaa tttatgccaa
     1141 ttacacatgt ttctgcagtt ttaggcttag aaaaaatgat atcaggacaa tcaaaagttc
     1201 tttcggttaa tggtaaatat ggtgcagtca ttttaacgaa agctgattta ggattagaga
     1261 atgcaattac tgaactacct tatgcaagcg agacaagcga tggtattctt actgctgaaa
     1321 tgtttcaaaa gatcgtaaat ggagaaggtg gaacatatat tttgccgatt gctactcctg
     1381 aacaattagg aggtataaaa gttggggaac tcttagaagt tactgaagaa ggtgttttat
     1441 ctgcaactaa gcaaacagac ttcaatttct ctgaagaatt aaagggaaaa ttagaatctc
     1501 taaaaatatt aaaagcaggt gcaaatatat caattgcaga agatggaaca attagttcta
     1561 caggtggtag tgggactggt ggtgttaatc agtcatatgt agatcaaaaa ttccaagaag
     1621 ctgtaaatca agcagaaaat tacacaaacg aaagaattcc aaattttact tttgaaaaaa
     1681 tcggggaggt atagaaaatg acagatatcg taaaaataaa acaaagcg
//
LOCUS       DAZKLM010000185         1721 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3764
            SAMEA115706913-rid22582563.denovo.193, whole genome shotgun
            sequence.
ACCESSION   DAZKLM010000185 DAZKLM010000000
VERSION     DAZKLM010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706913
            Sequence Read Archive: ERR13196647
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1721)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1721)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287823.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287823.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,004
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,849
            CDSs (with protein)               :: 2,849
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 41 of 96
            Pseudo Genes (incomplete)         :: 52 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 15 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1721
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706913-rid22582563.denovo.193"
                     /isolate="VRE3764"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     regulatory      161..327
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00380"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="The M-box (ykoK) riboswitch appears to function as
                     a genetic 'off' switch that is important for maintaining
                     magnesium homeostasis in bacteria; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="magnesium"
                     /db_xref="RFAM:RF00380"
     gene            501..1607
                     /locus_tag="ACG842_002790"
     CDS             501..1607
                     /locus_tag="ACG842_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HFQ0249258.1"
                     /translation="MIGLLEVYSASSYRLLQADENTKSLLLRQLIFIFLSWSVIFLAR
                     SVKLHYLLHPKIAGYGLALSIFFLVLVRIGIFGVTVNGAQRWISLFGIQFQPSELANL
                     FLIFYLSWFFRDGNSSPKDLKKPFLITVGITFLILFQPKIAGALMILSIAWVIFWAAA
                     VPFKKGIYLIVTFSALLIGAAGGVLYLGNKGWLPQMFNHAYERIATLRDSFIDSHGAG
                     YQMTHSFYALYNGGIFGRGLGNSITKKGYLPETETDFIFSIITEELGLIGALCVLFLL
                     FSLCMRIFCLSSRCKNQQAGLFLLGFGTLLFVQTIMNVGSIAGLMPMTGVPLPFVSYG
                     GTSYLILSLGIGITLNISSKIQAEELALYRPEKQ"
BASE COUNT          495 a          280 c          359 g          587 t
ORIGIN      
        1 atcctggtgc aactgaaaga atttgcaacg ggattctttc aacttatttg ttattttttt
       61 ctaaaaaata ttaaagattg ttaaatttca ctatttcatt tcaaattcca ttgcctaatc
      121 attaggaaaa gtctatcatg aactcgtaaa tagaatatag taataacctt gttaggtgag
      181 gctcctatac aaacataggc tgttgcccag aaacgtcgaa agtcgccaat gggtaaaaca
      241 gaaactgttg gattaaggct ttttccaata gctaagaatg ttttctttac gttgtatagt
      301 gctaaaactc ggacgatgag atgatgctag gtcagaattc ttgttcgaaa aggtttctga
      361 ccttttcttt atatttttgt gactgactta tttgatgaga caaactcgct cctcacctga
      421 caagtggatt gctgtgtgag gaggagatgc ggtgaaaaaa gatcgattgg tggattttgg
      481 gtccttattt gactttgtcc atgatcggtc ttttagaagt atacagtgcc agttcttatc
      541 gtttattgca agccgatgaa aatacaaaaa gtctattatt gcgtcaacta attttcatat
      601 ttttgagttg gagcgtgatc ttcttagctc gttcagtcaa actacactat ttacttcacc
      661 ctaaaatagc aggatacggt ttagccttat cgattttctt tttagtatta gtaagaatag
      721 ggatattcgg tgtcactgtc aacggcgcac aacgttggat ctctctgttt ggcattcaat
      781 tccagccttc tgaactggca aatctttttt tgatttttta tttaagctgg ttttttcgtg
      841 acggaaatag tagcccaaaa gatctaaaaa aaccattcct gattacagta ggtataactt
      901 ttctgatttt atttcagcca aagattgctg gagcattgat gatcctttcg attgcgtggg
      961 tcatattttg ggcagcggcg gttccattta aaaaagggat ctatctaatc gttacttttt
     1021 ctgcattgct gattggagca gcaggcgggg tattatattt aggaaataaa ggttggcttc
     1081 cacagatgtt taatcatgct tatgaaagga tcgcgacttt aagagattct tttatcgata
     1141 gtcatggtgc gggctatcaa atgacccatt cgttttatgc actatataat ggtggcatat
     1201 ttgggcgggg attaggaaat agtatcacaa aaaaagggta tcttccagag acagaaacag
     1261 attttatttt ttcgattatt acagaagagc ttgggctaat cggtgcattg tgtgtgcttt
     1321 tcttattgtt ctcattatgc atgagaatct tttgtttaag cagtcgctgt aaaaatcagc
     1381 aagctggatt gtttctttta ggattcggaa ctttgctatt cgttcagacg ataatgaacg
     1441 tgggaagtat cgcggggctg atgccaatga ctggtgtgcc attgccattt gttagctacg
     1501 gaggaacaag ttatctgatc ctttctttag gaatcggaat cacgttgaat atttcttcaa
     1561 agatacaggc cgaagagttg gcgctctatc gacctgaaaa acaataaaga gaaactgaga
     1621 aactctcagt ttgaattatc ataaagtaca atcttttatt tttcaagcga gaggttcttt
     1681 tagaaagagg aattcgcaat gaaagtatct caaaaaatca t
//
LOCUS       DAZKLM010000186         1664 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3764
            SAMEA115706913-rid22582563.denovo.194, whole genome shotgun
            sequence.
ACCESSION   DAZKLM010000186 DAZKLM010000000
VERSION     DAZKLM010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706913
            Sequence Read Archive: ERR13196647
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1664)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1664)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287823.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287823.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,004
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,849
            CDSs (with protein)               :: 2,849
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 41 of 96
            Pseudo Genes (incomplete)         :: 52 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 15 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1664
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706913-rid22582563.denovo.194"
                     /isolate="VRE3764"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            131..505
                     /locus_tag="ACG842_002791"
     CDS             131..505
                     /locus_tag="ACG842_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HFQ0249259.1"
                     /translation="MYLKITEQAQERLQKYIDEGATVILDLDDGVGEYSKMGVCSLDT
                     SFRLLLLDKEQRKNDYKLQLDSDIGDVYIKDYSKMYMDEHMTLSLDPRLGVFTLESPS
                     GSLDSHVQLVDLRPEKFSGIKQ"
     gene            complement(619..1248)
                     /locus_tag="ACG842_002792"
     CDS             complement(619..1248)
                     /locus_tag="ACG842_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFQ0249260.1"
                     /translation="MKIVFLHGLGQDEQSWKDVISYLPNSYSCKSLSLFNHLNSTDTA
                     TLDDLTKYIEVQLNQIKEPFILCGLSLGAVIALNYLATPSPYLKKVVLVAPQFESPNR
                     LLLTFQNIYFYCVPSKIFEKLGLSKKQTLTLLRSLETLDCKKQLRTSRLPKLILCGTK
                     DHANKPAAKRLYKLSTNGRLLFIKNGKHELNREKPEIMAQAMRSFIENS"
     gene            complement(1382..1454)
                     /locus_tag="ACG842_002793"
     tRNA            complement(1382..1454)
                     /locus_tag="ACG842_002793"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1419..1421),aa:Thr,seq:ggt)
BASE COUNT          584 a          240 c          283 g          557 t
ORIGIN      
        1 ctacactctg agatgtaaga aatatcataa gatatttctt acatcttttt gttttgagca
       61 ggactttaga taattttatt aatatatgct agcatgcttt tgtaagaaaa agaaagaggg
      121 cgtattagtt atgtatctta aaattacgga acaagcacaa gaaagattac aaaaatatat
      181 tgatgaaggc gcaacagtta tattggattt agatgatggc gttggggaat attctaaaat
      241 gggtgtttgc tctttagata ctagttttcg tttacttctt cttgataaag aacagagaaa
      301 aaatgattat aaactccaat tagatagtga tattggggat gtttatataa aagattattc
      361 aaaaatgtac atggatgaac atatgaccct ttctttagat ccacgtctag gtgtatttac
      421 tcttgaaagc ccaagtggtt ctttagacag tcatgttcaa ttagtagatc tacgaccaga
      481 aaaattctct ggaataaaac aataactttc tctctattgt ccataaaact agtctcaatc
      541 aagctgtatt gaaaaaacaa gacaacattc ttaccatggt aaataaaaag acgacacatt
      601 ttgtcgtctt tttttaaatt aactattttc gataaaagat cgcatagctt gtgccattat
      661 ttctggcttt tctctattca attcatgttt tccgttttta ataaagagta gccggccatt
      721 cgttgaaagt ttatataaac gttttgctgc tggtttattc gcatgatctt tggtaccgca
      781 taaaatcaat ttaggaagtc gtgatgtacg aagctgtttt ttgcagtcta atgtttcaag
      841 tgaacgaagt agggtaaggg tttgtttttt agatagacct aatttttcaa atatcttaga
      901 aggtacgcag taaaaataaa tattctggaa agtaagcaat aagcgattag gagactcaaa
      961 ttgtggagcg actaaaacaa ccttttttaa atatggagaa ggagttgcaa gatagtttaa
     1021 agcaataaca gcgcctaaag ataatccaca taaaataaaa ggttccttta tctgattcaa
     1081 ttgtacttcg atgtatttgg ttaagtcgtc taaagtagca gtgtctgtgc tgtttaaatg
     1141 attgaacaat gaaagacttt tgcaagaata actattgggt agataagaga ttacatcttt
     1201 ccatgattgt tcgtcttggc ctagaccatg caaaaaaaca attttcatat tgctctcctt
     1261 tttatagtta tcatatagac tttctttgta ctattaaaat ataaacattc atttcttacg
     1321 tcataaaata aaaaaacagc gcagacacat tgttaaacca acatgttgca ctgtttaagg
     1381 aagccacttg ccgggcttga accggcgacc tcttccttac catggaagcg ctctaccagc
     1441 tgagctaaag cggcattatc attaatacaa ataataggat aacgaatatt aatgtatttg
     1501 tcaacagaat tttttcgaaa aaatgtgttt ctctaatagg aaaacagatt ttagaaaaaa
     1561 taaaaataac aaaaaatgcg aacggtttct ttgataatat aataaaaaaa ttcaaaaaaa
     1621 gtattgcgtt atttagaaga aattgatatt atattcatgt actt
//
LOCUS       DAZKLM010000187         1658 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3764
            SAMEA115706913-rid22582563.denovo.195, whole genome shotgun
            sequence.
ACCESSION   DAZKLM010000187 DAZKLM010000000
VERSION     DAZKLM010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706913
            Sequence Read Archive: ERR13196647
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1658)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1658)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287823.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287823.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,004
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,849
            CDSs (with protein)               :: 2,849
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 41 of 96
            Pseudo Genes (incomplete)         :: 52 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 15 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1658
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706913-rid22582563.denovo.195"
                     /isolate="VRE3764"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            176..808
                     /locus_tag="ACG842_002794"
     CDS             176..808
                     /locus_tag="ACG842_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HFQ0249261.1"
                     /translation="MIFPKVNEASLNKGSFFHIYENEDFELYNILSQKEVIENQIICH
                     PIIYKSIFKYAFFASNDFQRMEITDCIFENCDFSGCILTKSMLHRVKFVNCKLTGTKL
                     MESYIGNTLFENCKMDYVNLSGSNIKESTFEHSVLSSADFVDCSLTKIMFSTNDLENA
                     SFFDTDLKNIDLSSNSFEMIEVRSEHLKNCTLNQLQALGFARKFLQIKVV"
     gene            931..1569
                     /locus_tag="ACG842_002795"
     CDS             931..1569
                     /locus_tag="ACG842_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family glutamic-type intramembrane
                     protease"
                     /protein_id="HFQ0249262.1"
                     /translation="MNNILGKKIELKKGILGTIVVVLGLLGLLFAPSIISIFSLTITG
                     FIAFTLVFGKKDTKKMFSKPVAPVKNIAKYFFINVVISAAVSVFLQQILKWGLTGNPI
                     NEAFSPLLFIILPIMILGEELFSVYFLAIFSSKFSIPVASFLSAIIFGFIHYSTYDNG
                     NILHTVAHILLIQGVARLLFNQAAIKSNSIITSWAVHVIFDFTAILLVVLFS"
BASE COUNT          559 a          251 c          262 g          586 t
ORIGIN      
        1 aagattttta ttagaattac gcctaatttg tgtctctcag ggcaattcta agaacgtttc
       61 aagagatagc gacactatcg ttatcgaaaa aggcgaaaac ctatctcatt ttctgctaat
      121 gtaagttggt ttagcttttg ttaatatgac tttatgaaaa tggaggtaat taaatatgat
      181 ttttcctaaa gttaatgaag caagcttgaa taaaggatcc ttttttcata tctatgaaaa
      241 cgaagatttt gaactgtata atatcctttc acaaaaagag gtcatcgaaa accaaataat
      301 ctgccatcca attatttaca aatctatttt caagtatgca ttttttgctt ccaatgattt
      361 tcaaagaatg gagattaccg actgtatatt cgaaaactgt gatttttcag gctgtatatt
      421 gacaaaaagc atgttgcatc gcgtgaaatt tgtcaattgt aaattaacag gtactaaatt
      481 gatggaaagt tatattggga acacgttatt cgagaattgt aaaatggact atgtaaattt
      541 atctggttca aacataaaag aaagtacctt tgagcactct gtcttatcaa gcgcagattt
      601 tgtagactgt tccctcacta aaataatgtt ttctactaat gatttagaaa atgctagctt
      661 ttttgatact gatttaaaaa atatcgattt aagtagcaac tcctttgaaa tgattgaagt
      721 acgctcggaa catctaaaaa attgtacgct taatcagttg caagcacttg gttttgctag
      781 aaaattttta caaataaaag tagtataatc aaaatgaata tataagataa atttttagca
      841 atacaaatat ccataaaaaa acattgccta ttttaactct tcatgtatac tgactctatc
      901 ttcaaaaact gaatagtagg agttattatc atgaataaca ttttaggaaa aaaaatagaa
      961 cttaaaaaag ggatattggg cactattgtc gttgtactcg gtttgttagg gttgctattt
     1021 gcgccatcca ttattagcat tttttcttta actatcactg gatttatagc atttacactt
     1081 gtttttggta aaaaagatac gaaaaaaatg ttctcgaaac ctgtcgctcc tgtcaaaaat
     1141 atagccaaat atttttttat caatgtagta atcagtgccg cagtctcggt atttttgcaa
     1201 caaattttga aatggggttt aacaggcaat ccaataaatg aggctttcag cccgttatta
     1261 tttattattt tgcctatcat gatacttgga gaagaattgt tctcagtgta ttttctcgct
     1321 attttttcta gtaagttcag cattcctgtc gctagtttct tgtcagcaat catctttgga
     1381 tttatccatt acagtactta tgataatgga aatattttac acactgtagc acacatcctt
     1441 ttgatacaag gggtggcaag attgttattc aaccaagctg ccattaaaag caactcgatc
     1501 attacaagtt gggcagttca tgtgatattt gatttcacag ctatattgct agtcgtttta
     1561 ttctcctaaa tttttataga aataatacat gctctattgc taagaaagca tactttattt
     1621 cctttaaaca aaaagacaga tgtatcaatg gattaaaa
//
LOCUS       DAZKLM010000188         1628 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3764
            SAMEA115706913-rid22582563.denovo.196, whole genome shotgun
            sequence.
ACCESSION   DAZKLM010000188 DAZKLM010000000
VERSION     DAZKLM010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706913
            Sequence Read Archive: ERR13196647
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1628)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1628)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287823.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287823.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,004
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,849
            CDSs (with protein)               :: 2,849
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 41 of 96
            Pseudo Genes (incomplete)         :: 52 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 15 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1628
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706913-rid22582563.denovo.196"
                     /isolate="VRE3764"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            23..505
                     /locus_tag="ACG842_002796"
     CDS             23..505
                     /locus_tag="ACG842_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HFQ0249263.1"
                     /translation="MIDLAKKFLHENIYFELYHHRAIYTNEDALVIKKEQGFTGTETK
                     SLFLKDKKDNNYVYLTFTTKQTDFKALGKIVGKRLSIVSSERMEEQTGQKQGAVSPFG
                     YEKQIPIIIDEELLSNEKLVFAPGRPDQTMVIQTVDLNKIIQLLEMPIFLMPANEKNN
                     "
     gene            542..1552
                     /locus_tag="ACG842_002797"
     CDS             542..1552
                     /locus_tag="ACG842_002797"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S66 peptidase family protein"
                     /protein_id="HFQ0249264.1"
                     /translation="MISYPLLERNATIGVTAPSSGVSEEHHSLLESALKRMEEKSFQV
                     VTGKTLWTQDKEKSGPARKRADEFNQMMQDENIQMIFPTWGGELLIEILDFVDFDALQ
                     PKWIVGYSDISTLLLAVTLKTGIATAHGTNLIDLRGEYLDETTAMWESVLKTREGESI
                     LQQSSEKYQKVWPHEHSSSSVFHLTEPTNWQSVSGDAVRMKGRLLGGCIDTIRHLIGT
                     PYGDVQSFKRKYNDGKPILWFLENCELSSVDLRRSLVQMKLAGWFDGSSGIMFGRSAA
                     EDLLDYKMKDVYEDLSKELKLPILYDIDCGHVPPQLTLINGAYAEVAVEDRKATVLQT
                     FI"
BASE COUNT          588 a          254 c          350 g          436 t
ORIGIN      
        1 aaaatagaaa agagacataa aaatgattga cttggcaaag aaattcttac acgaaaatat
       61 atattttgag ttgtatcatc atcgagccat ttatacaaat gaagatgctt tggtaataaa
      121 gaaagaacaa ggatttacag gtacagagac caaaagctta ttcttaaaag ataaaaaaga
      181 taataactac gtatatctga cttttactac gaaacaaacg gatttcaaag cattagggaa
      241 gatcgtcggt aaaaggcttt ctattgtttc atcagaacgc atggaagaac aaacgggaca
      301 aaagcagggc gctgtttctc cgtttggtta tgaaaaacag attccgatta ttattgatga
      361 agagctctta tcaaatgaaa aacttgtttt tgcaccaggt agaccagatc aaacgatggt
      421 catccaaacg gttgacttaa acaaaatcat ccagctactt gagatgccga tatttttgat
      481 gccggcaaac gaaaaaaaca attaaccaag taaaatagtg catacttgga ggtgaagcaa
      541 catgataagt tatccattat tagaaagaaa tgcaacgatt ggggttactg caccatcctc
      601 aggcgtatca gaggaacacc attcgttact cgaatcagct ttaaagagaa tggaagaaaa
      661 atctttccaa gtagttactg gaaagacgct ttggacgcaa gataaggaaa aatcaggtcc
      721 agcaagaaaa cgagcagatg aattcaatca aatgatgcaa gatgaaaata tccaaatgat
      781 ctttccgact tggggaggag aattattgat tgagattctc gattttgtcg attttgatgc
      841 tctacaacca aaatggatag taggttattc agatattagt acgttgctac ttgctgtcac
      901 attaaaaaca gggatagcca ctgcacatgg aactaacctt attgatttaa gaggagagta
      961 cttggatgaa acgacagcaa tgtgggaaag tgtactgaaa acaagagaag gagaatcgat
     1021 tctacagcaa tcttctgaaa agtatcaaaa agtgtggccc catgagcatt catcatcttc
     1081 tgttttccat ctcactgaac caacaaattg gcaatcagtc agcggagatg cagtaaggat
     1141 gaagggacga ttattaggtg gttgtattga tacgatcagg catttaatcg gtactcctta
     1201 tggagatgtc caatctttta aaagaaagta taatgatgga aagccgatct tgtggtttct
     1261 agaaaactgt gagctatcgt cagttgattt aagaagatca cttgttcaga tgaaactggc
     1321 aggctggttt gatggcagct cggggatcat gttcggtaga agtgcggcag aggatttgtt
     1381 agattataaa atgaaggatg tatacgaaga tctgtcaaag gaattaaaac taccgatctt
     1441 gtatgatatt gattgtggtc atgtaccgcc acaactgaca ttgattaatg gtgcctatgc
     1501 agaagtagcg gtagaagata gaaaagcgac tgttttgcaa acgtttattt aaaaaatttt
     1561 ttcctgcctt ggagtctatc aaagaccaat aaatctaaat agaaaagaaa gtgaaagaag
     1621 ctgacaaa
//
LOCUS       DAZKLM010000189         1624 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3764
            SAMEA115706913-rid22582563.denovo.197, whole genome shotgun
            sequence.
ACCESSION   DAZKLM010000189 DAZKLM010000000
VERSION     DAZKLM010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706913
            Sequence Read Archive: ERR13196647
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1624)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1624)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287823.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287823.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,004
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,849
            CDSs (with protein)               :: 2,849
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 41 of 96
            Pseudo Genes (incomplete)         :: 52 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 15 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1624
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706913-rid22582563.denovo.197"
                     /isolate="VRE3764"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(213..1544)
                     /locus_tag="ACG842_002798"
     CDS             complement(213..1544)
                     /locus_tag="ACG842_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFQ0249265.1"
                     /translation="MGKSSRKDIVFEKFGIVATKLGNQIHLRTLRDAFATFMPFMMLA
                     GFVTLINSVILEPTGFMGEIVDTSTLTTLQQIGTSIANGTLSITTLLVVAAVSYHMCA
                     SRNYTNHIAAVLVAISTFVVLTPMQMMFTPENADKAVEVTGVIPISYTGASGMFVGIF
                     VGLAATELFIKLSNNKKLQIQLSGNIPPAVLKSFNVLIPIIITITSFALLSFIIIQLF
                     NMDINKLITTIVTEPLSKITTGLPGFLLITSIANLFFGFGIHQAVISGSLLDPFLIQN
                     MQENMAAYANKEQIPHIINMAFKDTFAVMGGSGNTIALLIAIFIFSRRKDYKDFAKLS
                     VTPAIFNISEPIIFGLPIVFNISLIIPFVLAPIFSLTVAYFATAVGLINHVVVQIPWT
                     TPPVISGFLATAGDWRAAVLQILIIAASVFIYLPFLRIDEHVTTKLSENET"
BASE COUNT          563 a          295 c          234 g          532 t
ORIGIN      
        1 atttttcacc accaaaaacc tatctttgca tatactaaat agacgtatat acacagatag
       61 gtttgtcttg tgaaaaataa tgattatcct taccaaaaaa tgtttgtttt tctacatgtt
      121 cgtacttatc aaaaaatatg atccttattg aaattactgg ctatttatat tccaacgact
      181 tcttgtgtta atttttgtta tctcatagta aattatgttt cattttcact taatttagtt
      241 gtcacgtgtt cgtcaattcg taaaaacggt aaatatataa acacacttgc ggctataatt
      301 aatatttgta aaacagctgc tcgccagtct ccggctgttg ctaaaaatcc cgatataact
      361 ggtggcgtag tccaaggaat ttgcactact acatgattaa ttaatcccac agcagttgca
      421 aaatatgcta ctgtcaaaga aaaaatcggt gcaagcacaa agggaataat cagactgata
      481 ttaaatacaa taggcaatcc aaaaataata ggttcgctta tattgaatat agcgggagtt
      541 accgataatt tagcaaagtc cttataatct tttctacgac tgaagataaa tattgcaatc
      601 aataaagcaa tcgtatttcc cgatccaccc attaccgcaa aggtatcttt aaaggccata
      661 ttgataatat gtggaatttg ctctttgttt gcataagctg ccatattttc ttgcatattc
      721 tgaatcaaaa acggatctaa caatgaacct gatattactg cttgatggat accaaatcca
      781 aaaaatagat tagcaataga tgtaattaac aaaaatcctg gtaaaccagt tgtaatttta
      841 cttaatggct ctgtaactat cgtggtaatt agcttattta tatccatatt aaacaattga
      901 atgattataa atgacaataa agcaaacgag gtaatcgtaa taatgattgg gattaataca
      961 ttaaaagact ttaaaacagc gggaggaata tttccggata gctgaatctg tagtttctta
     1021 ttattagaaa gtttaataaa taattctgta gccgctaatc caacaaatat acctacaaac
     1081 attcctgaag ctcctgtata ggatataggt atcacacccg tcacttcaac agctttatcc
     1141 gcattttcag gagtaaacat catctgcata ggtgttaata caacaaatgt cgaaatagca
     1201 actaataccg ctgcaatatg atttgtataa tttctgcttg cacacatatg ataagatact
     1261 gctgctacaa ctaataatgt agttatgctt aatgtaccat tggcaattga agtaccaatt
     1321 tgttgtaacg tggtcaatgt actagtatcg acaatttccc ccatgaatcc tgttggttct
     1381 aagatcactg aattaataag tgtcacaaat cctgctaaca tcataaatgg cataaatgta
     1441 gcaaacgcat ctcgcaaagt tcttaaatgg atctgattcc ccaacttagt cgcaactata
     1501 ccaaattttt caaatacaat gtccttcctg ctacttttgc ccatattttt tctcctttat
     1561 gcttattgta atcgattact ttttccaccc aaataataca tcacttatca aaaaaagtaa
     1621 atac
//
LOCUS       DAZKLM010000190         1612 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3764
            SAMEA115706913-rid22582563.denovo.198, whole genome shotgun
            sequence.
ACCESSION   DAZKLM010000190 DAZKLM010000000
VERSION     DAZKLM010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706913
            Sequence Read Archive: ERR13196647
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1612)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1612)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287823.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287823.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,004
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,849
            CDSs (with protein)               :: 2,849
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 41 of 96
            Pseudo Genes (incomplete)         :: 52 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 15 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1612
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706913-rid22582563.denovo.198"
                     /isolate="VRE3764"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(578..1192)
                     /locus_tag="ACG842_002799"
     CDS             complement(578..1192)
                     /locus_tag="ACG842_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFQ0249266.1"
                     /translation="MSLCYFDYYHQLEEKLRIEEPLMLQAGTWIAKQINQGGRLQIYA
                     SRTLSGVAFEFWDQTPDLVPSILIENPAGGVYETLEGAGQAIIDQLSVKSEDIFLLLS
                     NEGRNPAIIELAQWIKENGHPLIIVTGFDLSRSIKSQHSSGLRLFEFADLVLDNHANL
                     HDAVLTLPNLDPAICGTASLATIFLLQQVLYFTAKQLVQDTSGK"
BASE COUNT          529 a          299 c          252 g          532 t
ORIGIN      
        1 ttccttaaaa ttcttttttt atttttagat tagagaaaat ctcgtcctgt ttttttcatt
       61 ttacaatcat tgaaaaattg aatttcattg caaaaaaaat aataaacact tataaatatc
      121 cattatctat ccttcaattt ttgtgtattt tatttctatt agaaaaaggt atcctgttgg
      181 acaattttgt tatatcaggt aaacttcatt gcaataatga taacaaatgt actataatta
      241 ctttgtaaag agttttgggg aagactcttt catcaacgtg tattaagaga caagaaagaa
      301 tccatatgga ttctttcttg tctctttttc cactactttt tatttttctg acactacata
      361 ttcttataac tcatttctca taactgtgaa acaacttata aaaataacaa actttaaaca
      421 aaacaagctt aaaatcaaag atgatccatc ttaaaataac taaaacaaaa aaagtagtcg
      481 ttgttaatcc tcattaataa tcgttttctt tttttcaaac gctccatttt tatgtggaac
      541 attatttttt taaggaactt tgtcaacatg tggataacta ttttccactt gtgtcctgga
      601 ctagctgttt tgctgtaaaa tacaaaactt gctgcagcaa aaagatcgtt gcaagagagg
      661 ctgttccgca gatcgctgga tctagatttg gcaaagtcaa gactgcatca tgtaaattgg
      721 cgtggttgtc caaaaccaaa tcagcaaatt caaataatcg caaaccactg ctatgttgcg
      781 atttgatact tcgtgacaaa tcaaatccag taacaatgat taatggatga ccattttctt
      841 tgatccactg agctagttcg atgattgccg ggtttcttcc ttcattcgaa agaagaagaa
      901 aaatgtcttc tgatttgaca gaaagctgat caataattgc ctgtcctgca ccttctagcg
      961 tttcatagac acctccggct ggattttcaa tcaatatact tggtacaaga tccggggttt
     1021 gatcccaaaa ttcaaatgct acaccactta gtgtgcggct agcatatatc tgcaatctac
     1081 caccttgatt aatttgtttc gcgatccaag tgccagcttg aagcatcagc ggctcttcta
     1141 tccttaattt ctcttccaat tggtgataat agtcaaaata acataaactc atttatctct
     1201 cccgcctagc tatacttgac atgaatagaa acagctgcct aatctaagga agtacataaa
     1261 aaaacagtag ttagacagta acacatgtgg tctttgctgt ttcaaacaca aaaaaggcat
     1321 tgaacgaata cgcccaatac cttgacatag caaataaaac aacgtgcaga ccatggaaaa
     1381 tatctacata ggggtagaat tttcacccta gattaggtac aataaattag gtcgatgcac
     1441 tgcattgact atggcaaaat gatcggtcag ggtgtatgtg tgtgactggt cattccctga
     1501 ttcttagcgc caactaaggg tcagggagcc tttttttatt cagctgtttc tatctgaatt
     1561 tattgtaagt aatctcttat ccaagtgcaa gcaagaaaat acaaaaaaac at
//
LOCUS       DAZKLM010000191         1536 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3764
            SAMEA115706913-rid22582563.denovo.199, whole genome shotgun
            sequence.
ACCESSION   DAZKLM010000191 DAZKLM010000000
VERSION     DAZKLM010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706913
            Sequence Read Archive: ERR13196647
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1536)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1536)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287823.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287823.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,004
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,849
            CDSs (with protein)               :: 2,849
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 41 of 96
            Pseudo Genes (incomplete)         :: 52 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 15 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1536
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706913-rid22582563.denovo.199"
                     /isolate="VRE3764"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..>1536)
                     /locus_tag="ACG842_002800"
     rRNA            complement(<1..>1536)
                     /locus_tag="ACG842_002800"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          321 a          476 c          337 g          402 t
ORIGIN      
        1 gggaaatctc atcttgaggt gggcttcaca cttagatgct ttcagcgttt atcccttccc
       61 tacatagcta cccagcaatg cccttggcag aacaactggt acaccagcgg taagtccatc
      121 ccggtcctct cgtactaagg acagctcctc tcaaatttcc aacgcccgcg acggataggg
      181 accgaactgt ctcacgacgt tctgaaccca gctcgcgtgc cgctttaatg ggcgaacagc
      241 ccaacccttg ggaccgacta cagccccagg atgcgacgag ccgacatcga ggtgccaaac
      301 ctccccgtcg atgtggactc ttgggggaga taagcctgtt atccccaggg tagcttttat
      361 ccgttgagcg atggcccttc catgcggaac caccggatca ctaagcccga ctttcgtccc
      421 tgctcgactt gttggtctcg cagtcaagct cccttctgcc tttacactct tcgaatgatt
      481 tccaaccatt ctgagggaac ctttgggcgc ctccgttacc ttttaggagg cgaccgcccc
      541 agtcaaactg cccatctgac actgtctccc accacgatta gtggtgcggg ttagagtggc
      601 cataacgcag gggtagtatc ccaccagcgc ctccatcgaa actagcgttc cgatttctac
      661 ggctcctacc tatcctgtac atgcggtaca gacactcaat atcaaactac agtaaagctc
      721 catggggtct ttccgtcctg tcgcgggtaa cctgcatctt cacaggtact aaaatttcac
      781 cgagtctctc gttgagacag tgcccaaatc gttacgcctt tcgtgcgggt cggaacttac
      841 ccgacaagga atttcgctac cttaggaccg ttatagttac ggccgccgtt tactggggct
      901 tcaattcgta ccttcgcttg cgctaagcac tcctcttaac cttccagcac cgggcaggcg
      961 tcagccccta tacttcatct tacgattttg cagagacctg tgtttttgat aaacagtcgc
     1021 ttgggcctat tcactgcggc tgatcgtatg atcagcaccc cttctcccga agttacgggg
     1081 tcattttgcc gagttcctta acgagagttc tctcgctcac cttaggattc tctcctcgac
     1141 tacctgtgtc ggtttgcggt acgggccgct gttttctcac tagaagcttt tctcggcagt
     1201 gtgacatcag gaacttcggt actattattt ccctccccat cacagcttgt ccttaagaaa
     1261 agaagcattt gactcctctt cagacttact gcttggacag acatttccga tcgtctgcat
     1321 tccttagcct cctgcgtccc tccattgctc aaacaaaaac aacgggtaca ggaatatcaa
     1381 cctgttatcc atcgcctacg cctgtcggcc tcggcttagg tcccgactaa ccctgggcgg
     1441 acgagccttc cccaggaaac cttagtcatt cggtggacag gattctcacc tgtctttcgc
     1501 tactcatacc ggcattctca cttctaagcg ctccag
//
LOCUS       DAZKLM010000192         1535 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3764
            SAMEA115706913-rid22582563.denovo.200, whole genome shotgun
            sequence.
ACCESSION   DAZKLM010000192 DAZKLM010000000
VERSION     DAZKLM010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706913
            Sequence Read Archive: ERR13196647
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1535)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1535)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287823.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287823.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,004
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,849
            CDSs (with protein)               :: 2,849
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 41 of 96
            Pseudo Genes (incomplete)         :: 52 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 15 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1535
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706913-rid22582563.denovo.200"
                     /isolate="VRE3764"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1026
                     /locus_tag="ACG842_002801"
     CDS             <1..1026
                     /locus_tag="ACG842_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HFQ0249267.1"
                     /translation="LTGNPKNIRLFLYNLFNSTEISFVELYTLPIIIKTEKMLKDLKQ
                     YYPLSAYEKKKLFHFLAINLLAEQADSLDIASNQSLFFSDIGFYHLKGVLGEKSVIFI
                     NKTLSWLYLHDKLDQKYLRKNEDETIEWLNERFIESFENQFNLLREETSQLIRRELTK
                     IHFKLLHYTINKADEFPLDVSFFRENYPAFYCYLIEYINDLTSKRKELLQDKFFLFFS
                     YLLLLINHIPIQLISEPVKIIIDFSYGAAYNQFIKKNLSLYINLNTEVIEAGKPEFPD
                     VIITNRNNLYEESASQVVVWLDPPRAVDWGNLTKILLTIQEEKYKDSKIDKQIDDFPE
                     EIIDRIE"
     gene            complement(1080..>1535)
                     /locus_tag="ACG842_002802"
     CDS             complement(1080..>1535)
                     /locus_tag="ACG842_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFQ0249268.1"
                     /translation="TIVGIHHKSAVITLVERLSKAIIALKPEGRKAVDIENSINEWLQ
                     SVPKNLFKSITFDCGKEFSNWKSISNTNDIDIYFADPGTPSQRGLNEHSNGLLRKDGL
                     PKEMEFNQVNQGFISSVASKRNHIPRKSLNYQTPLEVFLSYVNGKFCLA"
BASE COUNT          510 a          238 c          273 g          514 t
ORIGIN      
        1 ttgacaggga atcctaaaaa cattcggctt tttttatata atctttttaa ctcaacagag
       61 ataagttttg tggaattgta tacgctacct ataattataa aaacagaaaa aatgttaaaa
      121 gatttaaaac agtattatcc tctgtctgct tatgaaaaaa agaaattatt ccacttttta
      181 gcaatcaacc tgttggctga acaagctgat tctttagata ttgctagcaa tcagtcgcta
      241 tttttttcgg atataggatt ttaccactta aaaggagtat taggagaaaa atccgttatt
      301 tttatcaata agacactctc ttggctttat cttcatgata aattggatca aaaatacttg
      361 agaaaaaatg aagatgaaac aatcgaatgg ttgaatgaac gattcattga aagttttgaa
      421 aatcagttta atctgttgcg agaagagacg agccaattga tccgaagaga gttaaccaaa
      481 atacatttta aacttttgca ttacactatt aataaagctg atgaatttcc acttgatgtt
      541 tcttttttta gagaaaacta tccagcattt tattgctatt tgatagagta tataaatgat
      601 ctaacgtcaa aacgcaaaga gttgcttcaa gataaatttt ttcttttttt cagttattta
      661 ttattattga tcaatcacat acccattcaa ctgatttctg aaccagtaaa gatcatcatc
      721 gatttttctt atggtgctgc ctataatcaa tttatcaaga aaaatttaag cttgtacatt
      781 aatctgaaca cggaagtgat agaggcagga aagcctgagt tcccggatgt tataatcacg
      841 aataggaata atctatatga agaaagtgca tctcaagtag tggtttggct cgatccgcca
      901 agagcagtag actgggggaa tcttacaaag attttgttga ctatccaaga agagaaatac
      961 aaagacagca aaattgacaa gcaaatagac gattttcctg aagaaatcat cgacaggatc
     1021 gaatagtgaa cttcttttag aaagtccgac tgctttttct aaaaatatta tttgtcaaat
     1081 taagcgagac aaaactttcc atttacgtaa ctcaaaaaaa cttctaatgg tgtttggtaa
     1141 tttagtgatt ttctagggat atggtttctt ttggacgcaa cggatgagat gaatccttga
     1201 ttgacttggt tgaattccat ttcttttggt agtccatctt ttcggaggag tccgtttgaa
     1261 tgttcattta atccccgttg ggaaggcgtt cctgggtctg cgaaataaat atcaatatca
     1321 ttcgtattac taatactttt ccaattagag aattcttttc cacaatcaaa ggtaattgat
     1381 ttgaaaagat ttttgggtac ggattgaagc cattcattaa tcgaattttc aatatcaact
     1441 gccttacggc cttctggttt caaggcgata atggcttttg aaagtcgctc aacgagtgtg
     1501 atgacggcac ttttatggtg gatgccgaca atcgt
//
LOCUS       DAZKLM010000193         1513 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3764
            SAMEA115706913-rid22582563.denovo.201, whole genome shotgun
            sequence.
ACCESSION   DAZKLM010000193 DAZKLM010000000
VERSION     DAZKLM010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706913
            Sequence Read Archive: ERR13196647
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1513)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1513)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287823.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287823.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,004
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,849
            CDSs (with protein)               :: 2,849
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 41 of 96
            Pseudo Genes (incomplete)         :: 52 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 15 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1513
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706913-rid22582563.denovo.201"
                     /isolate="VRE3764"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            3..1469
                     /locus_tag="ACG842_002803"
     CDS             3..1469
                     /locus_tag="ACG842_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HFQ0249269.1"
                     /translation="MEKQQDKGFFGQPKGLSTLFFTEMWERFSYYGMRALLIYYMYDT
                     VANGGLGLPRATALAIMSIYGSLIYMSSIVGGWVSDRVLGAKRTVFFGGVFIMLGHIV
                     LALPFGVSTLFVSMALIIIGTAFLKPNVSGMVGHLYSKTDLRRDAGFSIFYMGVNLGA
                     LLAPLIVGTIGQEYNYHLGFSLAAIGMFFGLLQYIIQGRKTLDGVGTTPPNPLTTEER
                     KGFIRNLIIALVVIAAVFGGAQLTGHLTIDFFVNTISVLGIILPLYYFIKMLTSKDVT
                     AEEKPRVWAYIPLFLAAIIFWSLQEQGSSILALFASERTQSTLFGVTIQASWYQSLNP
                     IFIFILTPVFVTMWTKLGERQPSTVVKFSLGLLFAGLSYLLLMLPGMLYGTSGKVSPL
                     WLLGSFFIVVIAELCLSPVGLSVTTKLAPRAFESQTIAIWFLADASSQAINAQIARFY
                     TPGTESAYFGIIGAVAIVGGILLFTVKEPIKKLMGSIH"
BASE COUNT          414 a          289 c          319 g          491 t
ORIGIN      
        1 ttatggaaaa acagcaagac aagggcttct tcgggcagcc gaagggattg tcaactttgt
       61 tcttcactga gatgtgggaa cgatttagtt attacggtat gcgtgcatta cttatctatt
      121 atatgtacga tacagttgcc aacgggggac ttggattacc acgagcaaca gcattagcta
      181 tcatgtcgat ctacggatct ttgatctaca tgtctagtat cgtcggcgga tgggtatctg
      241 accgagtgtt aggagcaaaa cgcactgtat tcttcggtgg agttttcatt atgcttggcc
      301 acatcgtctt ggctttacca tttggtgtaa gtacattgtt tgtttcgatg gcattgatta
      361 ttatcggaac cgcctttttg aaaccaaatg tttctggcat ggtcggacat ctttattcaa
      421 agactgactt gcgaagagat gcaggatttt cgatttttta catgggtgtc aacttagggg
      481 cattattagc accactaatc gttggtacaa tcggtcaaga atataactat catttagggt
      541 tctctttagc tgctattggg atgttctttg gattattaca atacatcatc caaggacgca
      601 agacattaga cggagtgggt acaacaccgc cgaatccttt gacaactgaa gaacgtaaag
      661 gattcatccg taacttgatc atcgcacttg ttgttatcgc tgcagtattc ggcggggcac
      721 aactaactgg tcatctgaca attgactttt ttgtcaatac aatcagtgta ttggggatta
      781 tcttaccgtt gtattacttt atcaaaatgc tgacttcaaa agatgtaact gctgaagaaa
      841 aaccaagagt atgggcttat atcccattat tccttgcagc aatcattttc tggtcattac
      901 aagaacaagg ttcatctatc ttagctttat ttgctagtga acgtacacaa tctacgctat
      961 ttggtgtaac gatccaagca agctggtatc aatcattgaa tccaattttc atctttattc
     1021 tgacaccagt attcgtaacg atgtggacga aacttggtga acgtcaacca tctacagtag
     1081 tgaaattctc tcttggactt ttatttgcag gattatcgta cttgctattg atgttgccgg
     1141 gtatgcttta tggtacaagt ggaaaagtaa gtccattgtg gttgttggga agtttcttca
     1201 tcgtggttat tgctgaattg tgtctttcac cagttggttt gtctgtgaca acaaaattag
     1261 caccacgagc atttgaatca caaacaatcg ctatttggtt cttagctgat gcttcttcac
     1321 aagcaatcaa tgcacaaatt gcacgcttct acacaccggg aacagaatct gcttacttcg
     1381 gtattatcgg tgcagtagca atcgtcggtg gtatcttgct atttactgtg aaagaaccaa
     1441 tcaaaaaact aatgggctct attcattaat caatgaacat aaaaacagga attgcttatt
     1501 tgcagcagtt cct
//
LOCUS       DAZKLM010000194         1474 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3764
            SAMEA115706913-rid22582563.denovo.202, whole genome shotgun
            sequence.
ACCESSION   DAZKLM010000194 DAZKLM010000000
VERSION     DAZKLM010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706913
            Sequence Read Archive: ERR13196647
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1474)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1474)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287823.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287823.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,004
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,849
            CDSs (with protein)               :: 2,849
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 41 of 96
            Pseudo Genes (incomplete)         :: 52 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 15 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1474
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706913-rid22582563.denovo.202"
                     /isolate="VRE3764"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1410
                     /locus_tag="ACG842_002804"
     CDS             <1..1410
                     /locus_tag="ACG842_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rib/alpha-like domain-containing protein"
                     /protein_id="HFQ0249270.1"
                     /translation="PTVEGEKVEVGGTVDLTDNVTNLPALPEGTTVTDVTPGGTIDTN
                     TPGNYEGVIEVTYPDGTKDTVKVPVEVTDNRSDADKYEPTVEGEKVEIGGTVDLTDNV
                     TNLPALPEGTTVTDVTPGGTIDTNTPGNYEGVIEVTYPDGTKDTVKVPVEVTDNRSNA
                     DKYNPEGQKVTTDLNKEPDASEGIKNKEDLPKGTTYAWKEKVDVSTAGNKKGTIVVTY
                     PDGSSDEVEVDVTVTDTRSDADKYNPEGQKVTTDLNKEPDASEGIKNKEDLPKGTTYT
                     WKEKVDVSTAGNKKGTVVVTYPDGSKEEVEVTISVVDKKAPNKPQVDPITEGDQIVTG
                     KTEPNAEVTVTLPDGSQHHGTADKNGNFTVKVPKLEAGTKVIVTATDESGNTSEPTNV
                     VVSSNKKDSGKNGSKGSKTDNQGSNSNQDKNRGKSQSSKVFPKTGESDSNIFTISGGL
                     ILLGTLGLLGYKNRKKENE"
BASE COUNT          581 a          233 c          322 g          338 t
ORIGIN      
        1 ccaacagtag aaggcgaaaa agtagaagtt gggggcacag tagatctaac agataatgtt
       61 actaacttgc cagcgttacc agaaggaaca acagtaacag atgttactcc cggcggtaca
      121 attgatacca acacaccagg taattacgaa ggtgtcattg aagtaacgta tccagacggt
      181 acgaaagata cggtgaaagt tccagtagaa gtgacagaca atcgttctga cgccgataaa
      241 tatgaaccaa cagtagaagg cgaaaaagta gaaattggag gcacagtaga tttaacagat
      301 aatgttacta acttgccagc gttaccagaa ggaacaacag taacagatgt tactcccggc
      361 ggtacaattg ataccaacac accaggcaat tatgaaggtg tcattgaagt aacgtatcca
      421 gacggtacga aagatacggt gaaagttcca gtagaagtga cagacaatcg ttctaatgca
      481 gataaataca atcctgaagg acaaaaagtg actacagact taaataaaga gccagatgca
      541 tctgagggaa ttaagaacaa agaagattta ccaaaaggaa ctacttatgc ttggaaagag
      601 aaagtagatg ttagcacagc tggaaataaa aaaggtacta ttgtagtgac atatccagat
      661 ggatcatctg atgaagttga agtagacgtt acagtgacag atactcgttc cgatgcagat
      721 aaatataatc ctgaaggaca aaaagtgact acagacttaa ataaagagcc agatgcatct
      781 gagggaatta agaacaaaga agatctacca aaaggaacta cttatacttg gaaagaaaaa
      841 gtagatgtta gtacagctgg aaacaaaaaa ggtactgtag tagtaacata tccagatgga
      901 tctaaagaag aagtagaagt tactatttct gtagtagata aaaaagcacc gaataaacct
      961 caagttgatc ctattacaga gggtgaccaa attgttactg gtaaaactga accaaatgca
     1021 gaggtgacag ttacattacc agacggaagt caacaccatg ggacagctga taaaaatggt
     1081 aattttacag ttaaagttcc taaattagag gcagggacaa aagtgatagt aactgcaact
     1141 gatgaatctg gtaatactag tgaacctact aatgtagttg tttcttccaa caaaaaagac
     1201 agtggaaaaa acggtagtaa aggtagtaag actgacaatc aaggtagcaa cagcaatcaa
     1261 gacaaaaata gaggcaaaag ccagtcctca aaagtttttc ctaagacggg agagagtgac
     1321 agtaatatct ttactattag cggtggttta atactgttag gaacgttagg attattaggg
     1381 tataaaaatc gaaaaaaaga gaatgaatag aaaatcaatt tcttaagtct tctctgagaa
     1441 gaggaagaat ctttttcttc ctctttttgc tttt
//
LOCUS       DAZKLM010000195         1461 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3764
            SAMEA115706913-rid22582563.denovo.203, whole genome shotgun
            sequence.
ACCESSION   DAZKLM010000195 DAZKLM010000000
VERSION     DAZKLM010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706913
            Sequence Read Archive: ERR13196647
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1461)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1461)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287823.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287823.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,004
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,849
            CDSs (with protein)               :: 2,849
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 41 of 96
            Pseudo Genes (incomplete)         :: 52 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 15 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1461
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706913-rid22582563.denovo.203"
                     /isolate="VRE3764"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(225..1244)
                     /locus_tag="ACG842_002805"
     CDS             complement(225..1244)
                     /locus_tag="ACG842_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HFQ0249271.1"
                     /translation="MKKKITITAMSLLTALFLLPINGFAYTINNEFNLGANEGSSQVA
                     NNQYILLHETANETATGRNEAQYMQRSWTSAYTAYIVGDGGIVYQVGQPGYVQYGAGS
                     YANANSPVQIELQHTHDKATFEKNYKAYVELARDSAMKYGIPLTLDTPYNQPGIKSHL
                     WVTQNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAAN
                     PTLTDGTNYAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIY
                     RSDVNQAYNTSGNVGYHVSFNMRNFPNKKVYVMMRATNDPEGNTKGGAQDFHDKRWYL
                     NIPKR"
     gene            complement(1241..>1461)
                     /locus_tag="ACG842_002806"
     CDS             complement(1241..>1461)
                     /locus_tag="ACG842_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HFQ0249272.1"
                     /translation="NKTFEVLKWFALVIIPALATFVGLVGKALNWQYTDICVVIITGF
                     GAFLGSVLGVSNRTYKMFSAESEEGGNK"
BASE COUNT          411 a          304 c          268 g          478 t
ORIGIN      
        1 tgagccgtct gctctgacca actgagctaa gggtactggt tgttgccaca taaagccata
       61 aacaatcaac cagtagaatg tgtggcaaca aacctgttat cgcatagctt ggagtgtgac
      121 tattatgggt gatagtgaag atatgcgata acatcactat tttatcgaat gatttttata
      181 gttgtcaata tagttatgta atgctcctca acgaggagct atttttatcg tttaggaata
      241 tttaaatacc aacgtttgtc atggaaatct tgcgcaccgc ctttagtgtt tccctctgga
      301 tcattcgttg cccgcatcat gacatagact ttcttattag ggaagttacg catgttaaaa
      361 gatacatgat agccaacatt tccagaagta ttataagctt gatttacatc tgatctataa
      421 attccatcag ctcttactcg agctaattct ttcccagtat tgtaatccat aatgaagata
      481 tactcgtatt tatagttagc aatgtgccat ccagctacat gcaaatttgc attttcgatt
      541 tctccaaact gatcaatgtg ggcgtaattc gttccatctg tcagtgtagg attagctgca
      601 ccagctcgtg ttggatcaat gacaggcttg ttttcagaag ttgttggatt ttcatcggta
      661 aatccatgag ctaaatcata tgctaatttt tctttactta cgcccatttc agaaagataa
      721 ccgtaaggat ctgtatgatc gccccagatg ttttgtgtta cccataaatg cgatttgatt
      781 cccggttggt tataaggagt gtccaacgtt aatggaatac catatttcat tgctgaatct
      841 ctagccaatt caacgtatgc cttgtagttt ttctcaaacg ttgctttatc atgtgtgtgt
      901 tgtaactcaa tctgcacagg actgttggca ttagcatacg aaccagcacc gtactgtaca
      961 taaccaggtt gaccgacttg ataaacaatt ccgccgtctc ccacaatata agcagtataa
     1021 gcgctagtcc atgaacgttg catatactgc gcttcattgc gtcctgttgc tgtttcatta
     1081 gccgtttcat gcagtaaaat atactgatta tttgctactt gtgagctacc ttcatttgcg
     1141 cccaaattaa attcattgtt gatagtatag gcaaatccgt taattggcaa taaaaaaaga
     1201 gccgttaaca ggctcatcgc agtaatagta attttctttt tcatttgttt cctccttctt
     1261 cgctttcagc cgagaacatt ttgtaggttc gatttgatac acccaacaca ctccctaaaa
     1321 acgcgccaaa accagtaatg atgacaacac agatatctgt gtactgccaa ttgagcgctt
     1381 taccaactaa ccccacgaaa gtagctagtg cgggaataat taccagtgcg aaccatttta
     1441 gtacttcgaa cgttttatta t
//
LOCUS       DAZKLM010000196         1437 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3764
            SAMEA115706913-rid22582563.denovo.204, whole genome shotgun
            sequence.
ACCESSION   DAZKLM010000196 DAZKLM010000000
VERSION     DAZKLM010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706913
            Sequence Read Archive: ERR13196647
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1437)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1437)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287823.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287823.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,004
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,849
            CDSs (with protein)               :: 2,849
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 41 of 96
            Pseudo Genes (incomplete)         :: 52 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 15 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1437
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706913-rid22582563.denovo.204"
                     /isolate="VRE3764"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            52..462
                     /locus_tag="ACG842_002807"
     CDS             52..462
                     /locus_tag="ACG842_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HFQ0249273.1"
                     /translation="MIKVYTSPSCTSCRKARAWLQANQLEFEEKNIFAEPLTESEIKK
                     ILAATEGGVQDIISTRSKIYEKLGIDFNELTLKQMIVLFKEYPSLLRRPILIDEKRIL
                     IGFNEDEIRSFVPREIRCVARNLIYLQNNNILNS"
     gene            476..832
                     /locus_tag="ACG842_002808"
     CDS             476..832
                     /locus_tag="ACG842_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HFQ0249274.1"
                     /translation="MEVKVYGSAHCVTSLKAKQWLEHHQLPYQFVDLEERDLSTEEAE
                     ELCSFEEVQAEQLFATWSEAFHNIAIDLSCVGKKQLIFLCTQHTNLLRRPLIIIDEVL
                     FIGYNEQLMEQLILNK"
BASE COUNT          531 a          210 c          268 g          428 t
ORIGIN      
        1 tttaaaagaa attactgtag aattggatta taattaaaaa ggagtgagaa aatgataaaa
       61 gtatatactt cgcctagctg tacttcctgt agaaaagcaa gagcatggtt gcaagcgaat
      121 caacttgaat ttgaagagaa gaatattttt gccgaaccgt taacagaaag tgagatcaaa
      181 aagatcttgg cagcgactga aggaggagtt caagatatta tttccactcg gtcgaaaatt
      241 tacgaaaagc tgggcataga ttttaacgaa ttaactttaa agcaaatgat cgtcttattc
      301 aaagaatatc cttcattatt gagacgacct attttgattg atgaaaaaag aatactaatc
      361 ggatttaatg aagatgagat ccggtcattt gtccctcgtg aaattcgatg tgttgccaga
      421 aatctgatct atttacaaaa caataacatc ttaaattcat agggagggag ccttcgtgga
      481 agtgaaagtc tatggatcag cgcactgtgt aacaagtctg aaggcgaaac aatggttaga
      541 acaccatcaa ttgccttatc aatttgttga cttagaagaa cgcgatctaa gtacagaaga
      601 agcagaagaa ttatgcagtt ttgaagaagt gcaagcagaa cagctatttg cgacttggtc
      661 tgaggctttt cacaacattg cgattgacct ttcctgtgta ggaaaaaaac aactgatttt
      721 tttatgcaca caacatacca atctcctcag acgtcctttg attattatcg atgaagttct
      781 atttattgga tacaatgagc agttgatgga acagcttatc ttaaataaat agactttaac
      841 tgttttaatc aagtcactga gcatttcata cagttgtaac agtcgaacac ttagcatcaa
      901 gcgtgttatg tgttcttttt ttgactttat ttttctacta tagagacatg ctcaatttat
      961 gtggtcaaaa ttcaaatcta ggtggggata tagagttaaa aatatattga catgtgtaaa
     1021 tttacatgat atattcattt ctgttgtttt aagtagcaaa gcaataaaat agtattttaa
     1081 aaaacatata aaatagttgt tgacatgtta gaaatgacat gataagataa ctgagttgtt
     1141 aaaaaagaac cacttgtgaa gcttgattct atcgaaaaaa acttcaaaaa agttcttgac
     1201 aaataacaaa tgataagtta tgataaatga gttgctgtaa gatttcgaaa aacgaaatca
     1261 aaaaaacttt taaaaaagag ttgacaatta cttgtctctc tgatatgata taaaagttgc
     1321 tacggcagcc aaaaacgaat cggggaacac tcctgatgaa gtagaccttt gaaaactgaa
     1381 caaagtaaga caaaccaaat gtgtagggcg tcttgattca attcaagaca acaaaca
//
LOCUS       DAZKLM010000197         1417 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3764
            SAMEA115706913-rid22582563.denovo.205, whole genome shotgun
            sequence.
ACCESSION   DAZKLM010000197 DAZKLM010000000
VERSION     DAZKLM010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706913
            Sequence Read Archive: ERR13196647
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1417)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1417)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287823.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287823.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,004
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,849
            CDSs (with protein)               :: 2,849
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 41 of 96
            Pseudo Genes (incomplete)         :: 52 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 15 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1417
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706913-rid22582563.denovo.205"
                     /isolate="VRE3764"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(150..1337)
                     /locus_tag="ACG842_002809"
     CDS             complement(150..1337)
                     /locus_tag="ACG842_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS16 family transposase"
                     /protein_id="HFQ0249275.1"
                     /translation="MTQVHFTFESEEIQAIINESGANDTAKTLMTIMFNQLMEEQRNQ
                     YIQANAYERSEERQSQRNGYYDRSFTTRIGTLELHVPRTRDGKFSPTIFERYQRSEKA
                     LIAAMIEMVISGVSTRKVTKTVELLTDGATVSKSFVSNLMKQLDPFVFEWRNRSLEGS
                     EYPFFMCDALYMKVRENHRIVSKGVYIGIGIDSDGRRTILGFDVQDGESEDNWDTVFQ
                     SFVQRGLFGVKLVISDAHKGLVKAVRKNFLGASWQRCQAHFLRNIFDKLPKKVSSDVK
                     DELKSIFKASELELTRERKEHFLEKYGCDSKLSAACDILENGFEDAIQILSFPENIRR
                     RIRTTNVLERLNEEIRRRERVIRIFPNINSITRIIGTLLMEKDTEWLASPRKYLEFNS
                     NNI"
BASE COUNT          412 a          308 c          249 g          448 t
ORIGIN      
        1 taaaagttca acctcatcaa aagcacaatg ttttgcgttg tgaacctaac tgccatgcct
       61 ttctttctga ggagagaggt caggagcagt agcgcagcga ctcttgatgt ctctcctcag
      121 aaagaagaga ctcgcacagg ttcatggtgc taaatgttat tcgaattaaa ttctaaatat
      181 tttcgtggag aagccagcca ctcagtgtct ttttccatca aaagtgttcc aattatccga
      241 gtgatggaat tgatatttgg aaaaatccga atcactcgtt ctctccggcg aatctcttcg
      301 ttcaaccgtt ccaacacatt ggttgtacga atccttcggc ggatattttc aggaaaagac
      361 aaaatttgaa tagcatcttc aaagccgttt tctaaaatgt cgcaagcagc acttaatttt
      421 gaatcacaac catatttttc caagaagtgc tcttttcttt cacgtgtcaa ttcaagttca
      481 gaggctttaa agatactctt caactcgtct tttacatcag aagaaacttt ctttggcagc
      541 ttatcaaaaa tatttctcaa aaagtgggct tggcatcttt gccagctcgc acctaaaaag
      601 ttcttgcgaa cagccttaac taaccctttg tgtgcatcag aaatgaccag ttttacaccg
      661 aacaaacctc gttgaacgaa cgattgaaaa acggtatccc aattatcttc cgattcgccg
      721 tcttgaacat caaaaccaag aatcgtacgt cgtccatcag aatcaatgcc gataccaata
      781 tatacacctt ttgagacaat acgatgattt tcccttactt tcatatacag ggcatcacac
      841 atgaaaaatg gatattccga gccttctagg ctccggtttc tccactcaaa aacaaatgga
      901 tccaactgtt tcatcagatt tgaaacgaat gatttagaaa cagtcgcacc atccgttagc
      961 agttcaactg ttttagttac tttgcgagtg gagacaccag aaatgaccat ttcaatcatg
     1021 gcagcgatca aagctttttc gcttcgctga tatctttcaa agatagtagg actaaactta
     1081 ccgtctctgg ttcgtggaac atgaagttct aacgtaccaa ttcgagttgt aaacgaacga
     1141 tcgtagtaac catttctttg tgattgccgt tcttctgaac gttcataggc atttgcctga
     1201 atatattgat ttcgttgttc ttccatcagc tgattaaaca tgatagtcat aagtgttttt
     1261 gcggtatcat tcgcaccgct ttcgtttata attgcttgaa tttcttcgct ttcaaaagta
     1321 aaatgtactt gagtcattga tatcagtcct attcgagtat gttttctgca aaaaacattg
     1381 tactcctaac tgagatcaat ggcttttttg atgcttc
//
LOCUS       DAZKLM010000198         1368 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3764
            SAMEA115706913-rid22582563.denovo.206, whole genome shotgun
            sequence.
ACCESSION   DAZKLM010000198 DAZKLM010000000
VERSION     DAZKLM010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706913
            Sequence Read Archive: ERR13196647
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1368)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1368)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287823.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287823.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,004
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,849
            CDSs (with protein)               :: 2,849
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 41 of 96
            Pseudo Genes (incomplete)         :: 52 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 15 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1368
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706913-rid22582563.denovo.206"
                     /isolate="VRE3764"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            82..>1368
                     /locus_tag="ACG842_002810"
     CDS             82..>1368
                     /locus_tag="ACG842_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISEfa11 family transposase"
                     /protein_id="HFQ0249276.1"
                     /translation="MNDSIKKMLRITEKDLMITEVSYETLQKKKTLVVDAVLSPTPRA
                     CRSCGSTVVDGNGKAIIVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQ
                     SYFVQPRHSIANHVRYKIASLLTEKVSLSFIAKSCQVSLTTVIRTLKEFKSYLPKQSK
                     KILPRVLMVDEFRSHASIEDKMSFICADGETGKLIDVLPTRKLPRLTSYFLGCTNPEE
                     VEFLVTDMNAAYFQLTKRVLPNAKVVIDRFHIVKHMNQAFNELRIREMNELRKAGQKS
                     QAEKLKKNWRFLLKNRANINHYEYKTWKSFRAPKYPFLTEAMMIDRLLEFSPPLKEAY
                     PFFHELVEAFRDKDPDLFFSLLAELPETLDDSFREKLQNLLTYEEGITNAMIYPYSNG
                     KIEAKNTHIKTMKRVSYGFKSFENMRIRIFLINQLIN"
BASE COUNT          467 a          263 c          257 g          381 t
ORIGIN      
        1 attttaggta ttaaaaaaca tatcgttctc tagtatgatt aaatcaccac aaaataaaca
       61 actgagagga cgatacgcta tatgaatgat tctatcaaaa aaatgctgag aataacagag
      121 aaagatttga tgattacaga ggtctcttac gagacccttc agaagaaaaa gacgttggtc
      181 gtcgatgctg ttctctcgcc tactcctcgt gcttgtagaa gttgtggttc tactgtggta
      241 gatggaaacg ggaaagcaat tatagtgaaa aatggaaaaa aggaaacgat tgtccgtttt
      301 gaacaataca atcatatgcc tttggttatg cgcctaaaaa agcaacgcta tacctgtaaa
      361 aactgtcgca cccattggac ggctcaaagt tattttgtcc aacccagaca ttcaatcgca
      421 aatcatgtta gatataaaat tgcttcttta ctgactgaaa aagtatcttt atcttttatt
      481 gcgaaaagct gtcaggtatc tttgaccacc gttattcgta cattgaaaga gtttaaaagc
      541 tatttaccaa agcaatctaa gaagatcctc cccagagtat tgatggttga cgaatttcgt
      601 tcgcatgctt ccatagaaga taagatgagc tttatttgcg cggatggcga aacaggaaaa
      661 ttaatagatg ttttgcctac gcgtaaatta cctcgattaa caagctattt cttaggttgt
      721 accaatccag aagaagtaga attcttggtg acagacatga acgccgccta cttccagctc
      781 accaaacgtg ttctgccaaa tgcgaaagtc gtgattgatc ggtttcatat tgtcaaacac
      841 atgaatcaag cgttcaatga gttgcgtatc cgtgaaatga atgaacttcg taaagccgga
      901 cagaaaagcc aggcagaaaa actgaaaaag aactggcgct ttttgctaaa aaatcgtgca
      961 aacatcaacc attatgaata caaaacatgg aaaagtttcc gagcaccaaa atacccattt
     1021 cttactgaag caatgatgat tgatcgattg cttgagtttt ctccgcccct aaaggaggcg
     1081 tatccctttt ttcatgaatt agttgaagcc tttcgagaca aagaccctga cttatttttc
     1141 tccttattgg cagaacttcc cgaaacgtta gatgacagct ttcgggaaaa gcttcaaaac
     1201 cttctgacct atgaagaagg catcaccaac gcaatgatct atccttattc caatggaaaa
     1261 atagaagcga agaataccca cataaagaca atgaaacgag tatcctacgg atttaaatca
     1321 tttgagaaca tgagaattag aatctttttg atcaatcaat taatcaat
//
LOCUS       DAZKLM010000199         1360 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3764
            SAMEA115706913-rid22582563.denovo.207, whole genome shotgun
            sequence.
ACCESSION   DAZKLM010000199 DAZKLM010000000
VERSION     DAZKLM010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706913
            Sequence Read Archive: ERR13196647
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1360)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1360)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287823.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287823.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,004
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,849
            CDSs (with protein)               :: 2,849
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 41 of 96
            Pseudo Genes (incomplete)         :: 52 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 15 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1360
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706913-rid22582563.denovo.207"
                     /isolate="VRE3764"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            4..978
                     /gene="bsh"
                     /locus_tag="ACG842_002811"
     CDS             4..978
                     /gene="bsh"
                     /locus_tag="ACG842_002811"
                     /EC_number="3.5.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase"
                     /protein_id="HFQ0249277.1"
                     /translation="MCTSITYVTSDHYFGRNFDYEISYNEVVTVTPRNYKLNFRKVND
                     LDTHYAMIGIAAGIADYPLYYDATNEKGLSMAGLNFSGYADYKEIQEGKDNVSPFEFI
                     PWILGQCSTVGEAKKLLKNINLANINYSDELPLSPLHWLLADKEKSIVIESMKDGLHI
                     YDNPVGVLTNNPSFDYQLFNLNNYRVLSSETPKNNFSNQISLNAYSRGMGGIGLPGDL
                     SSVSRFVKATFTKLNSVSGDSESESISQFFHILGSVEQQKGLCDVGDGKYEYTIYSSC
                     CNVDKGIYYYRTYEDSQITAIDMNKEDLDSHKLISYPIIEKQQIKYIN"
BASE COUNT          472 a          180 c          228 g          480 t
ORIGIN      
        1 actatgtgta cgtctattac ttatgtaaca agtgatcatt attttggaag gaattttgat
       61 tatgaaatat cttacaatga agtagtcact gttactccaa gaaattataa gttgaatttt
      121 cgaaaggtaa atgatttgga tactcattat gcaatgattg gtattgccgc tggtatagct
      181 gactaccctc tttattacga tgcgacaaat gaaaaaggat tgagtatggc tgggctaaat
      241 ttttctgggt atgctgatta taaagaaata caagaaggga aagacaatgt atctcctttt
      301 gaatttattc cttggatttt aggacaatgc tcaacagtag gagaagctaa aaaattgtta
      361 aaaaatatca atttagcaaa tataaattat agtgatgaac ttcctttatc ccctttacat
      421 tggctattag ctgataaaga aaaatcaatt gtcattgaaa gtatgaaaga tggacttcat
      481 atatatgata accctgtggg cgttcttacc aataatcctt catttgacta tcaattattt
      541 aatttaaaca attatcgtgt cttatcgagt gaaactccta aaaataattt ttcaaatcaa
      601 ataagtttga atgcctatag ccgcggtatg ggagggatag gcttgcctgg agatttatcc
      661 tcagtatctc gttttgttaa agcgactttt acgaagctga attctgtatc tggagattca
      721 gagtcagaaa gtattagtca atttttccat atcttaggtt cagtagaaca acaaaaaggt
      781 ttgtgtgatg ttggtgatgg aaaatatgaa tatacaattt attcttcttg ttgcaatgtt
      841 gacaaaggaa tctattatta tcgaacatat gaagacagtc aaattactgc aattgatatg
      901 aataaagaag acttagatag tcataagtta attagttatc caattataga aaaacaacaa
      961 attaaatata taaattagtt aatgtgttgt gattgattat ttaatataga tataatacaa
     1021 aaaggcaaag atattttcaa aacatttttt cttgatgtga tatccttgtc ttttttgtaa
     1081 tgtttttaac ttattgcttt tgcaaaattt atttacctgt tttctggatc aacgatagtg
     1141 tatattatgc tatccttgtt tggattttag gacgtttaaa gaggcacttt tttacataac
     1201 ttaatccata gaccttttaa gcatgctgtg agataataat aaagaagagt ttaaagcgca
     1261 ccccaaacca cttccccata agtgtattac gctttaaact cttttatatt tgaattcact
     1321 aagaagcata ccatattttt gaaaaaaagt gagaaaaaag
//
LOCUS       DAZKLM010000200         1356 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3764
            SAMEA115706913-rid22582563.denovo.208, whole genome shotgun
            sequence.
ACCESSION   DAZKLM010000200 DAZKLM010000000
VERSION     DAZKLM010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706913
            Sequence Read Archive: ERR13196647
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1356)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1356)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287823.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287823.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,004
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,849
            CDSs (with protein)               :: 2,849
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 41 of 96
            Pseudo Genes (incomplete)         :: 52 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 15 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1356
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706913-rid22582563.denovo.208"
                     /isolate="VRE3764"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            122..385
                     /locus_tag="ACG842_002812"
     CDS             122..385
                     /locus_tag="ACG842_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045927 - positive regulation of growth
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HFQ0249278.1"
                     /translation="MAKTVLNPSSARKNFYKLLKDVNENHTEIEIISERSENNAVLIG
                     LDDWKAIKETLLLEQIGTLGVVRKREKDDSGFTNVDEIDWDNL"
     gene            385..657
                     /locus_tag="ACG842_002813"
     CDS             385..657
                     /locus_tag="ACG842_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HFQ0249279.1"
                     /translation="MNNYSVMIKNSAKADLKKIKQSNLKPQFEKVIQTLKEDPYLPTQ
                     SFEKLKPTHEGRYSRRLNRQHRVVYKVDEDEHVVEIYSARTHYESQ"
     gene            734..1063
                     /locus_tag="ACG842_002814"
     CDS             734..1063
                     /locus_tag="ACG842_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFQ0249280.1"
                     /translation="MITKTRKQGNSIMLTVPKDFNVPNGVEVEAKLVENGILYEFVEP
                     QKEFYDFSEDILSDIIAEGYDKDEILVEFKNRKNKMHSSFRDIAEDTLTNSKVMTKEE
                     LATEIGL"
     gene            1053..1339
                     /locus_tag="ACG842_002815"
                     /pseudo
     CDS             1053..1339
                     /locus_tag="ACG842_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292679.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
BASE COUNT          512 a          162 c          271 g          411 t
ORIGIN      
        1 taaaggaaac ggataacact ttacaatgct gttttggtac tccaatttaa acaaaacaat
       61 cgaatttacg tacttaatta agtacgttag aatggagata taaattagag ggggagtaga
      121 tatggcaaaa acagtcttaa atcctagctc agcacggaaa aatttttata agttgttgaa
      181 agatgtaaat gagaatcata cggaaattga aatcattagt gagcgcagtg aaaataatgc
      241 ggtgcttatt ggtttggatg attggaaagc aatcaaagaa acgctattac ttgagcaaat
      301 aggcactttg ggtgttgttc gtaagcgaga aaaagatgat agtggattta ctaatgttga
      361 tgaaattgat tgggataact tataatgaat aactattctg tgatgattaa aaactcggct
      421 aaagctgatt taaagaaaat caaacagtct aatttgaaac ctcaatttga gaaggttatt
      481 cagactttaa aggaagatcc ctatctccct acccaatcat ttgagaagtt aaaacctaca
      541 catgaaggta ggtattctag aagattgaat agacaacacc gggttgttta taaggtagat
      601 gaagatgaac acgtggttga aatttattca gcaaggacgc attacgagtc acagtaatta
      661 cttgtaaata cattatctcg tggtataatg tatttactaa gtaattacat actgttagaa
      721 atgagggatt taaatgatta caaaaacaag aaaacaaggc aattcaatca tgttgacagt
      781 acctaaagat tttaatgttc ctaatggagt cgaagttgaa gcaaagttgg tagagaatgg
      841 tattctttat gaatttgttg aaccacaaaa agaattttat gattttagcg aagatatttt
      901 gtctgatata attgcagaag gctacgataa agatgagatt cttgtagaat ttaagaatag
      961 aaaaaacaaa atgcattctt cctttagaga tattgctgaa gatacactta ctaattctaa
     1021 agtaatgact aaagaggagt tggcaacaga aattggctta tgaaatcgtt ccttcaaaac
     1081 atgtcattaa gtatttaaaa agctaaaaga gaagccattg aaagaaaaat ttttgaattt
     1141 aatttatgat gaaattgcga ttgatccata taagggtgaa caaaaaactg gggatttatc
     1201 aggtatttgg tctagtgggt tcaaatatgc gggcacaaca tatagagtag cttacgagat
     1261 tatagaaaac aaggttattc cagtattgtt atgtggaacg catgaaaact tttatgaaca
     1321 gttgaaaaaa ttaatctagt gatttgttgt cgttct
//
LOCUS       DAZKLM010000201         1352 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3764
            SAMEA115706913-rid22582563.denovo.209, whole genome shotgun
            sequence.
ACCESSION   DAZKLM010000201 DAZKLM010000000
VERSION     DAZKLM010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706913
            Sequence Read Archive: ERR13196647
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1352)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1352)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287823.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287823.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,004
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,849
            CDSs (with protein)               :: 2,849
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 41 of 96
            Pseudo Genes (incomplete)         :: 52 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 15 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1352
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706913-rid22582563.denovo.209"
                     /isolate="VRE3764"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            85..624
                     /locus_tag="ACG842_002816"
     CDS             85..624
                     /locus_tag="ACG842_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0249281.1"
                     /translation="MWSLVGIVLIIFTVTAATEANYSHKIQSYKGRLVMTKKDVTRYT
                     SSRTGGLYDYAPLDALKYKDHLKKHEVKVGDRWENASHKAYNSKIVYTVEDADEQNIT
                     LPVFNYWGTVVKVNGKKSTIENDNGLVSIKGTGKRTIVEVSSTYPTTMILSLLISSIT
                     FIFLFTRYLIRCCGEMKID"
BASE COUNT          510 a          171 c          208 g          463 t
ORIGIN      
        1 agaaccttgg gttttcaagt gctttttggt tcattaatct taaccatagt gtttttaaag
       61 tggaaaacaa gcaacaaaaa aagtatgtgg agccttgtag gaatagttct tattattttt
      121 actgtgactg cagcaacaga agcaaattat tcgcataaaa ttcagtcgta taaaggtcgt
      181 ttagttatga ccaaaaaaga tgttacacgt tatacatcct caagaactgg aggcctatat
      241 gactatgcac cattagatgc attgaaatat aaagatcatt taaagaaaca tgaagtaaaa
      301 gttggtgatc ggtgggagaa tgcatctcat aaagcatata attccaaaat agtctataca
      361 gtagaagatg ccgatgagca aaatatcaca ttacctgtat tcaattattg gggaacagtt
      421 gtgaaagtaa atgggaaaaa gagcactata gaaaatgaca atggtttagt gtctattaaa
      481 ggtacgggga aaagaactat agttgaggtt tcatctacgt atccaactac gatgattcta
      541 tctttattaa ttagttcaat aacttttata ttcttattta cacgttattt aataagatgc
      601 tgcggtgaaa tgaaaataga ttagatagat attataaata tgttgattct attttcatag
      661 caatggagaa aatacattag caatatataa aagaaggtat caagagtcac agtgaattta
      721 ttccgaaaag tgaaaagagc aaatataaaa tttaatgaaa atatagaact aaataggaaa
      781 acgtagacgt aattttctac gttttccttt tcatatatat taaatattac ggaaaattat
      841 ataaactaat ttagtgtttt gtttgtaatt cctaattttg ctccactttt agataatttt
      901 taggtctatt taactcatca gacattctat gctcattttt ctccaaattt gtttcaactc
      961 tttcgtactt aattttgcgt atttgaaaat tcttctttta gagtactttt attacgatac
     1021 tagtatgata tatatctata gcaaacagtt aaaatcttcc taaaataagc gaatagcatt
     1081 cctaaaacct ataaaatttt tagatgaagc gacaagtgga caattaataa actacttgaa
     1141 catgaaatta tggatcacct ttcaaattta gaaaatggaa ttcttatact tattgcgcaa
     1201 catttattga ttgcaaaatt atgtgacaaa aaaaactagt agaagaaaga ataatggctt
     1261 gataataaag tactacctat taaaggtagt actttattta atttagtata aattaacata
     1321 ctactatatt ttaggtttca tattatataa at
//
LOCUS       DAZKLM010000202         1346 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3764
            SAMEA115706913-rid22582563.denovo.210, whole genome shotgun
            sequence.
ACCESSION   DAZKLM010000202 DAZKLM010000000
VERSION     DAZKLM010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706913
            Sequence Read Archive: ERR13196647
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1346)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1346)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287823.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287823.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,004
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,849
            CDSs (with protein)               :: 2,849
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 41 of 96
            Pseudo Genes (incomplete)         :: 52 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 15 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1346
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706913-rid22582563.denovo.210"
                     /isolate="VRE3764"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1244
                     /locus_tag="ACG842_002817"
     CDS             <1..1244
                     /locus_tag="ACG842_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HFQ0249282.1"
                     /translation="ITEKEHLLPLEEQFCEHCGSQMKDIGRTKVREEIRFHQAMLDCL
                     THYQHTYCCKSCEKEGLSSFKKAIVPKPLISNSLGSNSLVAETIRMKFGQKVPAYRQE
                     NYWKQTHGLDISRDNITNWHIKAVQNALDPLGERLRVYLNQEEILHGDETSYRVIESA
                     KTDTYYWQFCTGKDSQHPIVYYHHDESRAGDVPKTFLKEFTGYLHCDGYSGYNAVESV
                     RLVYCFAHVRRKFFEAIPKGKKNTDIPAAQAVKQLDKWFVLEKKWKDFSPEKRLSCRQ
                     QELRPLFIAFYEWMATIDPVAKSKLDAAVQYACKLRSGFEPIFEDGRLELTNNRAERN
                     IKELVIGRKNWLHSTSLEGARTSGIILSVYKTAELNGLNPVKYLEFLFDKIPNLPVLS
                     AETLDQLLPWNKDVQQHFSRN"
BASE COUNT          438 a          244 c          286 g          378 t
ORIGIN      
        1 ctataacaga gaaagaacat ctccttcctt tggaagaaca attttgtgaa cattgcggtt
       61 cacagatgaa agatatcgga cggacaaaag tacgagaaga aattcgtttc catcaggcta
      121 tgttggattg tctcacccat tatcaacata cctattgttg taaaagctgc gaaaaagaag
      181 gactttcttc ttttaaaaaa gcgatcgttc ctaaaccttt gatttctaat agcttaggtt
      241 ccaatagttt agtggcggaa acgatccgga tgaaatttgg tcagaaagtt cctgcctatc
      301 ggcaagagaa ctattggaaa caaacacatg ggttggatat ttcgagagat aatatcacca
      361 attggcatat taaagcagtg caaaacgcgt tagacccttt aggagaacga ttaagagtct
      421 atcttaatca agaagagatt ctacacgggg atgaaacaag ttatcgtgtg attgaaagtg
      481 ccaaaacaga tacctattac tggcagtttt gtacaggaaa agacagtcag catcccatcg
      541 tttattatca ccacgacgaa agtcgtgctg gagatgttcc gaaaactttt ctcaaggagt
      601 ttacagggta tttacactgt gacggctata gcgggtataa tgccgtggaa agtgtacgac
      661 ttgtctattg tttcgcgcat gttcggcgga aatttttcga agcaattcca aaaggaaaga
      721 aaaacacgga tattcccgca gctcaagcag tcaaacaatt ggataaatgg tttgtcctag
      781 aaaagaaatg gaaagacttt tctcctgaga agcgattaag ctgtcggcaa caagaactgc
      841 gtccattatt tattgctttt tatgagtgga tggcaacgat agatcctgtt gcaaaatcca
      901 agttggatgc agccgttcaa tatgcgtgta aactacgaag tggttttgaa cctatttttg
      961 aagatggtcg tttagaactc acaaataatc gtgcggagcg aaatatcaaa gagctggtga
     1021 taggacggaa aaattggttg cattccacaa gtcttgaagg agcacgaact tcaggaatca
     1081 ttctgagtgt atataaaaca gcagaactta acggattgaa tcctgtaaag tatctagagt
     1141 ttctatttga taagatacca aatcttcccg tactttcagc agaaacttta gatcaactgt
     1201 tgccttggaa caaggatgta caacaacatt tttctcgtaa ctaaaaacgc caaacattcc
     1261 cgattctatt ctataggtcg ggttatttga cgtacaggta ctgttctatg gggcgcttac
     1321 ttttcttctt caagaccaaa taaaaa
//
LOCUS       DAZKLM010000203         1326 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3764
            SAMEA115706913-rid22582563.denovo.211, whole genome shotgun
            sequence.
ACCESSION   DAZKLM010000203 DAZKLM010000000
VERSION     DAZKLM010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706913
            Sequence Read Archive: ERR13196647
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1326)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1326)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287823.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287823.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,004
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,849
            CDSs (with protein)               :: 2,849
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 41 of 96
            Pseudo Genes (incomplete)         :: 52 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 15 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1326
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706913-rid22582563.denovo.211"
                     /isolate="VRE3764"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(60..284)
                     /locus_tag="ACG842_002818"
                     /pseudo
     CDS             complement(60..284)
                     /locus_tag="ACG842_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877485.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(838..924)
                     /locus_tag="ACG842_002819"
                     /pseudo
     CDS             complement(838..924)
                     /locus_tag="ACG842_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339775.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
BASE COUNT          474 a          218 c          179 g          455 t
ORIGIN      
        1 ggtattttta ttatatatag atttaacata tgtcggtcgg ttgattgata tagctaaagt
       61 tataactcaa ccgaagccga ttcatctaca cgaataaagt tgcgagtgtt ctcggctaat
      121 tcataaaaat tgttatgtgt tccatatagc aatactggaa taactttatt ttcttgaatc
      181 tcataagcaa ctctatacac ggtgaccgca tacttaaatc cattagacca attacctgat
      241 aaacctccag ttttttgctc ttacttaaat ggatcaatgg caatttcatc acaaacctat
      301 agaggatgag tgacttctgc ctaattacgt taaaaaatat tgaaagtaac tctatgtagc
      361 aaaaaatatc tagaatttca tttcctcaaa actgatcgat attgtatcta aaaatatata
      421 ctacctaaaa taggtagtat atattaaaat atgtgctttt gttagaaaaa tcaatacata
      481 aattatcata tctgtatatg tcatttaggt atttcagtta tgttataata gttttgctgt
      541 ctgctacccc ggcctaatct ggcaagggga ggaggagata taatgaaata cctttttgat
      601 tttgtttttg caatcaagcc acttgcagat gtagtgtgca gttacctctg caaatggctt
      661 gattgcaaaa ttgccaaaaa agacagtaag cctacccgtt aattctataa tatagaaacg
      721 taaaaaaaac cccatcagcc tgcacgctga tggggtttgg tgatataatg aaatacctct
      781 ttgttactca taattatacc ataattagtc acgaatgcaa ccaatagaaa aaactactta
      841 gaacggtaga tcatcgctaa taacaatgca ttgtccacca aatggatttt gtgaagcatt
      901 aaatacattg ttattaattg tcgctgcttt taactaggcc tctccccttc tattataaaa
      961 gaagatatat atcatttctt aattacctac taccttaaac caaaatttaa gtactaccta
     1021 atttaagtag tacttaaaca atctaaaaaa tagttatccc aattgttatg cttttataat
     1081 acgattattt gtaacaaaat gaatattata taattaaaca ctaccatatt taggtagtgt
     1141 ttaattatta gcaaaacaaa aaatatttat tctattctct taacttcaac taagttatgc
     1201 tgcacacctt tatataaatc atatgcaact cattagctag acaaactaca agcatgtagt
     1261 tttatctgtt aattatgtac tacctaaata aagtagttta taattagcaa gtatatatat
     1321 ttaaaa
//
LOCUS       DAZKLM010000204         1314 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3764
            SAMEA115706913-rid22582563.denovo.212, whole genome shotgun
            sequence.
ACCESSION   DAZKLM010000204 DAZKLM010000000
VERSION     DAZKLM010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706913
            Sequence Read Archive: ERR13196647
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1314)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1314)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287823.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287823.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,004
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,849
            CDSs (with protein)               :: 2,849
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 41 of 96
            Pseudo Genes (incomplete)         :: 52 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 15 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1314
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706913-rid22582563.denovo.212"
                     /isolate="VRE3764"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            283..522
                     /locus_tag="ACG842_002820"
     CDS             283..522
                     /locus_tag="ACG842_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0249283.1"
                     /translation="MKRILFISLSNLLLLTACGTSASKNIPSDTQISNSIDSTISTNE
                     KNSPVETSDIKVSTSAKNRVKKLSARLLYFKQTLN"
     gene            complement(884..1144)
                     /locus_tag="ACG842_002821"
     CDS             complement(884..1144)
                     /locus_tag="ACG842_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0249284.1"
                     /translation="MNNETIILGKKYICQPIGLKQKVVGEVISKLENCVVICVDEYHD
                     VDRNEILEKCGKVVAKYTHVYELETTEYLYNYSNKYEHTLVS"
BASE COUNT          428 a          240 c          177 g          469 t
ORIGIN      
        1 ctatcctcct agcaactatt taaataccca aaaaaattac ctctagataa tatcatacct
       61 acgttttcac ttataaattt tttcataaaa aaagcgttta gaggcataat cgttaataca
      121 gatggtaacc aatgtatctg gtttgaactg ggatatcacc ctctccatat atgtattact
      181 aagactaata gatactataa aagacttgat agttttgcat tgattttgcc taatactgag
      241 cataggtgta tgctaaacga tactataagg agtgtgtaca taatgaaaag aattctgttt
      301 atcagtttat caaatttatt gttacttact gcttgtggga cttctgcatc aaagaacata
      361 ccttcagata cgcaaatttc gaatagtatc gattcaacaa tttctacaaa tgagaaaaat
      421 tctccggtag aaacttccga tataaaagtt tctacctctg caaaaaacag ggtaaagaaa
      481 ctgtcagcac gtctccttta tttcaaacaa actctgaatt agcttcttca tcttcttcag
      541 caaataatga tgatagtttc gcgtttactc ctaactaaat gcaaccttta caaatgcttt
      601 acgataacaa gcctgagcta aatgatcctg atatcagttt tgcatttttt gaaatgattg
      661 gatcggatga tctttttaaa actactagtt tgtctatccg aaaataggac agttctggca
      721 gtataggttt ttatcgagtt tctccaccag gcctcgttac agagacagat tctctaggcg
      781 aaccttttga ataaatcaaa aatcatgaac aatcactgcc aactaaataa agtgccttca
      841 aaagcttggt cttctcgctt ttgaaggcac tttatttaaa tctttatgaa actaaagtat
      901 gttcatattt gttgctatag ttatatagat attctgttgt ttccaactca taaacgtgag
      961 tatattttgc cactactttt ccgcattttt ctaatatttc attgcgatcg acgtcatgat
     1021 attcgtctac gcagatcaca acacaatttt ctaatttact gattacttct ccaaccactt
     1081 tttgtttcaa gccaataggt tggcaaatat atttttttcc taaaatgata gtttcgttat
     1141 tcatatttca tccatcctta atgtttttcc attatttaaa atgataatac cattaaaata
     1201 tgattatgaa aatcaataac acttgtgatt ttataaaaaa ataaaaaaac cgaaaaacag
     1261 aaatgcccac tgtctttccg gctcttttta ttcttgattt ctcttctttt ttgt
//
LOCUS       DAZKLM010000205         1287 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3764
            SAMEA115706913-rid22582563.denovo.213, whole genome shotgun
            sequence.
ACCESSION   DAZKLM010000205 DAZKLM010000000
VERSION     DAZKLM010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706913
            Sequence Read Archive: ERR13196647
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1287)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1287)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287823.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287823.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,004
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,849
            CDSs (with protein)               :: 2,849
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 41 of 96
            Pseudo Genes (incomplete)         :: 52 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 15 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1287
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706913-rid22582563.denovo.213"
                     /isolate="VRE3764"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..251
                     /locus_tag="ACG842_002822"
     CDS             <1..251
                     /locus_tag="ACG842_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HFQ0249285.1"
                     /translation="YTSRQQTPEVFEMNASMYSFKPEFLKKSNYIFDGQCGIIKMRDY
                     LILDIDSEEDFKWLEFLFPKFLEDSSELQDIYNYLYRR"
     gene            256..1203
                     /locus_tag="ACG842_002823"
     CDS             256..1203
                     /locus_tag="ACG842_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HFQ0249286.1"
                     /translation="MSCYLITGGAGFIGSTLANHLGEEHVVIVVDDLSMGKVENLDMT
                     KNITFIEGDVAEKSLMEEIMKANKFDYIFHLAAVASVADSVARPVETHRVNFESVLMI
                     LELIRKYQPDLKRIVFSSSAAVYGDEPTLPKKEESVIRPLTPYAVDKFAAEQYVLDYC
                     HLYDVPGSAVRFFNVYGPNQNPNSPYSGVISILVDRYKKQIVGEATSFTLFGDGSQSR
                     DFVYIDDVIQALLLVANEEAALGKQFNVGTGKATTLLELIHAINEILGVELTLEYKEE
                     RSGDIHDSLADITKIQSIGYQPKYGILSGMTNYLKTEIN"
BASE COUNT          422 a          187 c          267 g          411 t
ORIGIN      
        1 gatacacatc taggcaacag acacctgaag tttttgaaat gaatgcctca atgtattctt
       61 ttaaacctga atttctaaaa aaaagtaatt atatttttga tgggcaatgt gggataataa
      121 aaatgagaga ttatctaatt ttagatattg atagtgaaga agattttaaa tggttagaat
      181 ttttgtttcc taaattttta gaagattctt cggagcttca agatatctat aattatttat
      241 atcggaggta aaaggatgag ctgttatctt atcaccggtg gggctggctt cattggttct
      301 accttagcca atcatttagg agaagaacat gtggtaatcg tcgtcgatga cttatcaatg
      361 ggaaaagtag aaaatcttga tatgacgaaa aatatcactt ttattgaggg agatgtcgct
      421 gagaaatcct tgatggaaga gatcatgaaa gctaataaat ttgattatat ctttcattta
      481 gcagcagttg ctagtgtggc agattcagtt gcccgaccag tagaaacaca tcgtgtgaat
      541 tttgaaagtg tattaatgat tttagaattg atccgcaagt atcaacctga tttaaaacgg
      601 atcgtgttta gttcttcagc ggctgtttat ggggatgaac cgactttacc aaaaaaagaa
      661 gagtccgtga ttcgcccatt gacgccttat gcggttgata aattcgctgc agaacaatat
      721 gtgctagatt attgtcattt atatgatgta cccggtagcg cagttcgttt tttcaatgtc
      781 tatggtccaa atcaaaatcc gaattctcca tacagtggtg tgatttctat tctcgtagat
      841 cgctataaga aacaaatcgt aggagaagca acttcattta cattatttgg tgatggaagc
      901 cagtctagag actttgttta tatagatgat gtaatccaag cattactttt agttgcgaat
      961 gaggaagctg cactggggaa acaattcaat gttggaacgg gtaaagcaac cacacttttg
     1021 gaattgattc atgcaatcaa tgaaattctc ggtgtagaat tgacacttga atacaaagaa
     1081 gagcgttcgg gtgatatcca tgactctcta gcagatatta ctaagatcca gtcaattggc
     1141 tatcaaccga aatatggtat tttaagtgga atgactaatt atttgaaaac tgaaatcaat
     1201 taaaacatac taagataaga ttagagggta aattcaggct ctttgtcaat aaggactgat
     1261 gagttttgta aaatcaaatc tgggata
//
LOCUS       DAZKLM010000206         1284 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3764
            SAMEA115706913-rid22582563.denovo.214, whole genome shotgun
            sequence.
ACCESSION   DAZKLM010000206 DAZKLM010000000
VERSION     DAZKLM010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706913
            Sequence Read Archive: ERR13196647
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1284)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1284)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287823.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287823.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,004
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,849
            CDSs (with protein)               :: 2,849
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 41 of 96
            Pseudo Genes (incomplete)         :: 52 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 15 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1284
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706913-rid22582563.denovo.214"
                     /isolate="VRE3764"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..969
                     /locus_tag="ACG842_002824"
     CDS             <1..969
                     /locus_tag="ACG842_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan recognition family protein"
                     /protein_id="HFQ0249287.1"
                     /translation="PINTFAYTINDEYNLAPNQGDSRLAIPNKIILHETGIDAPARNV
                     AANMKNNYNGSNSYTTDVIGDGGIVYRVGEQGYVSWGAGNANPYAPVQIELQRTYDKA
                     LFEKNYRAYIEYTRDSAKKYGIPLTLDQGTSLFTKGIISHLWVTNYVWGNHTDPYGYL
                     SQMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAANPTLTDGTNHAHIDQFGE
                     IENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIYRPDVNQAYNTSGNVGYH
                     VSFNMRNFPSKKVYVMMRATNDPEGNTKGGAQDFHDKRWYLNIPKR"
     gene            1159..1232
                     /locus_tag="ACG842_002825"
     tRNA            1159..1232
                     /locus_tag="ACG842_002825"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1193..1195,aa:Ile,seq:cat)
BASE COUNT          423 a          229 c          262 g          370 t
ORIGIN      
        1 cccattaata cgtttgctta tactattaat gacgagtata atttagcgcc gaatcaagga
       61 gactccagat tagcaattcc taacaaaatt attttgcacg aaactggaat agatgcacca
      121 gcaagaaacg tagccgccaa catgaaaaat aattataacg gaagcaattc ttatactaca
      181 gatgttattg gtgacggtgg gattgtttac cgtgtgggtg agcaaggata tgtttcgtgg
      241 ggagctggta acgctaatcc ttatgcgcct gtacagattg aattacagcg cacatatgat
      301 aaagcattgt ttgaaaaaaa ctatcgagct tatattgaat atacaagaga tagtgcaaaa
      361 aaatatggaa ttccattgac tcttgatcaa ggaacttctt tatttacaaa aggaatcatt
      421 tctcatttgt gggtgacaaa ttatgtttgg gggaaccaca cagatccata tggttactta
      481 tcgcaaatgg gagttagcaa agaaaagctt gcttatgatt tagctcatgg atttaccgat
      541 gaaaatccaa caacttctga aaacaagcct gtcattgatc caacacgagc tggtgcagct
      601 aatcctacgc tgacagatgg aacaaatcac gcccacattg atcagtttgg ggaaatcgaa
      661 aacgcaaact tgcatgtggc tggatggcac attgctaact ataaatacga gtatattttc
      721 attatggact acaatactgg gaaagaatta gctcgagtaa gagctgatgg aatttataga
      781 ccagatgtaa accaagctta taatacttct ggaaatgttg gttatcatgt atctttcaat
      841 atgcgtaatt ttcctagtaa gaaagtctat gtaatgatgc gggcaacgaa tgatccagag
      901 ggaaacacta aaggcggtgc gcaagatttc catgacaaac gttggtattt aaatattcct
      961 aaacgataaa aatagctcct cgttgaggag cagtacataa ctatattgac aactataaaa
     1021 attattcgat aaaatagtga tgttatcgca tatcttcact atcacccata aatagtcaca
     1081 ctccaagcta tgcgataaca ggtttgttgc cacacattct actggttgat tgtttatggc
     1141 tttatgtggc aacaaccagt acccttagct cagttggtca gagcagacgg ctcataaccg
     1201 tccggtcgta ggttcgagtc ctacagggta cattaacgta gccatttgaa tcgttctgtg
     1261 ttagaatttt tttgaagagt atta
//
LOCUS       DAZKLM010000207         1274 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3764
            SAMEA115706913-rid22582563.denovo.215, whole genome shotgun
            sequence.
ACCESSION   DAZKLM010000207 DAZKLM010000000
VERSION     DAZKLM010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706913
            Sequence Read Archive: ERR13196647
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1274)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1274)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287823.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287823.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,004
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,849
            CDSs (with protein)               :: 2,849
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 41 of 96
            Pseudo Genes (incomplete)         :: 52 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 15 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1274
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706913-rid22582563.denovo.215"
                     /isolate="VRE3764"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(112..483)
                     /locus_tag="ACG842_002826"
     CDS             complement(112..483)
                     /locus_tag="ACG842_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0249288.1"
                     /translation="MFDFNEYLSISKQIESDDKYNSKESYRRTAVSRAYYSAFKKSDE
                     YLKENYDIIYNGSSGKGSHQTVWNLFSTVKELNTLGIHNSGYRMLEKRKCADYIPNET
                     ITKTDMALMNREAEKIINKLT"
     gene            complement(485..886)
                     /locus_tag="ACG842_002827"
     CDS             complement(485..886)
                     /locus_tag="ACG842_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0249289.1"
                     /translation="MLDIVPSNTHRSGSLINFAAIFACASVILASPVVVDASSTPKTK
                     NDNQLVITTKNEINTISSNDGKIFDVINTVLKRKAEINIKYDEDLNLYFFVIKTTSEL
                     FSSDYDVLDTLDNVLSDYKYMGKSVVATLGE"
     gene            complement(900..>1274)
                     /locus_tag="ACG842_002828"
     CDS             complement(900..>1274)
                     /locus_tag="ACG842_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0249290.1"
                     /translation="DVESQQFGTNVINKVINPALSINAPLIPTALSFSVTAVVSEIED
                     TEKIKIVEIEVLNKNEKQIFSTGEVSVSLPPQVNDINFNINARNVLVEEAGEHYAVVK
                     FNGTEIGRQIFDIKVNKPVEKN"
BASE COUNT          406 a          230 c          169 g          469 t
ORIGIN      
        1 ggacgcacgt agcgatcatc ggcgttatga ctatataaag tatactttcg ttgatccaga
       61 aagatacaaa gattaaataa aaaaataaca aacaaactcg cttgaaattc attaggtgag
      121 tttgtttatt attttttctg cttctcgatt catcagcgcc atatctgttt tcgtaatggt
      181 ttcattagga atatagtcag cacattttct tttctctaac attctatacc cactattatg
      241 tattcctaag gtattcaatt ccttaactgt tgaaaacaaa ttccaaacgg tttggtggct
      301 tccttttccg ctacttccat tataaataat atcataattt tctttcaaat actcatcaga
      361 ttttttaaaa gcactgtagt atgctcttga aacagcagtt cttctataag attctttaga
      421 attatattta tcatctgatt caatttgttt ggaaatagat aaatattcat taaagtcaaa
      481 cattttattc ccccaaagtt gctactacag atttccccat atatttatag tcagataata
      541 cattatcgag agtatccaaa acatcgtaat ctgaggaaaa caattcagat gttgttttga
      601 taacaaagaa atacaagttt aaatcttcat catattttat attgatttca gcttttcttt
      661 ttaaaaccgt atttatcaca tcaaaaattt tgccatcatt acttgaaatt gtgttaatct
      721 cattttttgt agtaattaca agttgattat catttttagt tttcggagta gaacttgcat
      781 ccacaacaac tggagatgct agaattaccg atgcgcaagc aaaaatcgca gcaaaattta
      841 ttaagctacc actccgatgt gtatttgatg gtactatatc aagcatccgt atcgtctcct
      901 taattttttt ccactggttt gttgaccttg atatcaaaga tttgccttcc aatctcagtt
      961 ccattaaatt taacaacagc ataatgctct cctgcttctt caaccaagac atttctggcg
     1021 tttatattaa agtttatatc gttaacttga ggaggcaagc ttaccgatac ttcacccgta
     1081 gaaaaaattt gtttttcatt cttgtttaaa acttcaattt ctactatttt tatcttttct
     1141 gtatcctcta tctcagacac cacagctgtt acagaaaatg ataaagcagt aggaattaaa
     1201 ggcgcattaa tagaaagagc tggattaatt actttattaa taacattagt gccaaattgc
     1261 tgactttcta catc
//
LOCUS       DAZKLM010000208         1260 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3764
            SAMEA115706913-rid22582563.denovo.216, whole genome shotgun
            sequence.
ACCESSION   DAZKLM010000208 DAZKLM010000000
VERSION     DAZKLM010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706913
            Sequence Read Archive: ERR13196647
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1260)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1260)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287823.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287823.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,004
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,849
            CDSs (with protein)               :: 2,849
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 41 of 96
            Pseudo Genes (incomplete)         :: 52 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 15 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1260
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706913-rid22582563.denovo.216"
                     /isolate="VRE3764"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(173..1030)
                     /locus_tag="ACG842_002829"
     CDS             complement(173..1030)
                     /locus_tag="ACG842_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HFQ0249291.1"
                     /translation="MVAKKYVIGNFKGGVGKSTCAQMLGFESAVNEGKRTLIIDLDMQ
                     GNTSDVMNLTHMNFTNEEGGGEGEPIVYENTVTDVLINNLPPKEAVYKVINNLYILPA
                     DMSFEMYDDWIKDQYNDSLSQFKYMKEKLEPLFDEFDVIYLDVPPSISVYSKSAMYIA
                     DWAIVVLQTQVKSMRNAMQYLEYMDFFVKEFDTNLYVAGVIPFMLESGDAVDQEMYKQ
                     AQEIYGEHLIKNVVLKNARLKRYDGSGITMEKTKKGLLKQWDRKSHELFISILKELEE
                     HENWYAEVE"
BASE COUNT          359 a          253 c          183 g          465 t
ORIGIN      
        1 tctgtctttc aacattggag gaagttttta tttcttgaac ttggtgcact tctaattgct
       61 tattttcaga tggtgtaatt gcttgctgct ttttctttgc ttctgcttct ttttttattt
      121 tggcgagtcc tttgttgttc ccaaaaatat caagtccttt gatgtcagcc aattactcca
      181 cctccgcata ccaattctcg tgttcttcca attctttcaa aatactgata aataattcgt
      241 ggcttttgcg atcccattgt tttaagagac ctttctttgt cttctccatc gtaatacctg
      301 atccgtcgta tcgttttaag cgcgcatttt tgagtacaac atttttaatc aaatgttccc
      361 cataaatctc ttgggcttgt ttatacattt cctgatctac tgcgtcaccg ctttccaaca
      421 tgaaaggaat aacaccagca acatataaat ttgtatcaaa ttctttcaca aaaaagtcca
      481 tatactcaag atattgcatc gcatttctca tcgattttac ttgggtctgt aagacgacga
      541 ttgcccaatc cgcaatgtac atcgcggatt tactatatac agaaatagaa ggtgggacat
      601 ctaaatagat gacgtcaaac tcatcaaata atggttctag cttttctttc atatatttga
      661 attgggataa tgaatcatta tattggtctt tgatccaatc atcatacatt tcaaaggaca
      721 tgtctgctgg aagtatatag aggttattga ttactttata gacagcctct ttaggtggaa
      781 gattgtttat caacacgtca gtcacagtat tttcgtaaac aatcggttca ccttctccgc
      841 ctccttcttc gttagtgaag ttcatatgcg ttaagttcat cacatcagaa gtatttcctt
      901 gcatatccaa gtcaattatc aaggttcttt ttccttcatt aactgctgat tcaaacccta
      961 acatttgcgc gcacgtactt ttacctacgc cacctttaaa attaccgatt acatatttct
     1021 tcgctaccac gttcctacct cttttctttt agcaatattc atttgatact attataacat
     1081 atttattttt agttactacc aaaaaaaaga tatatatgta ctaccttata attaataagt
     1141 aaaaggtagt acataaataa ggaaaagctg aaatatattg atttatagct ttcctcaaag
     1201 taatgacgtt atttaattta tcgagaagca gagaatactc gcgactttag tcatgtagac
//
LOCUS       DAZKLM010000209         1212 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3764
            SAMEA115706913-rid22582563.denovo.217, whole genome shotgun
            sequence.
ACCESSION   DAZKLM010000209 DAZKLM010000000
VERSION     DAZKLM010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706913
            Sequence Read Archive: ERR13196647
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1212)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1212)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287823.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287823.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,004
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,849
            CDSs (with protein)               :: 2,849
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 41 of 96
            Pseudo Genes (incomplete)         :: 52 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 15 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1212
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706913-rid22582563.denovo.217"
                     /isolate="VRE3764"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..799
                     /locus_tag="ACG842_002830"
     CDS             <1..799
                     /locus_tag="ACG842_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008754 - O antigen ligase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HFQ0249292.1"
                     /translation="MMNFIVQIFYITLSSSRGAQVVICSFLVLFLLLFANSKFRKSIR
                     EVLPVKIKWMVSILLVLLVYFSFGTNYAKDFLATIPVSIDRKVEVIESNESDKTPLPE
                     KEVILQHSSDNSEISAGRFTIWKAALKVIRPTLLFGYGETDFYGNSDAKFLKKVDLDT
                     TDVNELKRAHGNMHNGFLQVLVSSGIIAFICIYAFYVLNIFSLLKTFFRSKVNYAFLG
                     IVLIFILSIFIDEMVEAHVLFNKRDVISIVFWYYLGSISYLYLKKEN"
     gene            801..>1212
                     /locus_tag="ACG842_002831"
     CDS             801..>1212
                     /locus_tag="ACG842_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0249293.1"
                     /translation="MKTAFIAWTPLHLINILNVYQNFYRESKGDLFIYSEFENARRYS
                     ERIKELGLFNDVVFVNHRKIGSSFKRKINLLTNHNQFFSDDLSVYDEIFIQGENYFTK
                     ILFSNVYKKNPDVKLNYIEDGLGTYLKKSIFSTSK"
BASE COUNT          408 a          143 c          203 g          458 t
ORIGIN      
        1 tatgatgaat tttatcgtac aaatatttta tattacactt tcttcgtcta gaggtgccca
       61 ggtagtaatt tgttcttttt tagtattatt tcttttgttg tttgccaatt caaaatttag
      121 aaagagcatt agagaagtac tacctgtaaa aatcaaatgg atggtaagca tattattagt
      181 tctattggta tatttttctt ttggtactaa ttatgctaag gattttttag caacaattcc
      241 tgtatcgatt gatagaaaag tagaagttat agagtcaaat gaatcagata aaactccact
      301 tccagaaaaa gaagtaatat tacagcattc tagtgataac tccgaaattt cagctggtcg
      361 ttttacgatt tggaaagctg cgcttaaagt tataagacca actttgttat ttggttatgg
      421 agagaccgat ttctatggga atagcgatgc aaaatttttg aaaaaagtag atcttgatac
      481 tacagatgtg aatgaactaa aaagagcaca tggaaacatg cataatggat ttcttcaagt
      541 acttgtatca agtggtataa ttgcttttat atgtatctat gctttctatg tgttaaatat
      601 ctttagttta ctcaaaacat tttttagaag taaagtgaat tatgcgtttc ttggtattgt
      661 tttaattttt atactatcta tttttataga tgaaatggtg gaggctcatg tacttttcaa
      721 taagcgcgat gtcatttcaa ttgttttctg gtattatttg ggaagcattt cttacttgta
      781 tttaaaaaag gagaattaat atgaagacag ctttcattgc ttggactcct ttacatttga
      841 ttaatatatt aaatgtttat caaaattttt accgagaatc aaaaggagat ttatttattt
      901 attctgaatt tgaaaacgct agaagatata gtgagcgtat aaaagaatta gggctattta
      961 atgatgttgt ttttgtgaat caccggaaaa tcggttcttc ctttaaacgt aaaattaact
     1021 tgttaactaa ccataatcaa tttttctcgg atgatttatc cgtttatgat gaaattttta
     1081 tacaggggga aaattatttc acaaaaatac ttttctccaa tgtttacaaa aaaaatccag
     1141 atgtaaaact aaattatata gaagatggtt tgggtactta tttgaagaag agtatatttt
     1201 caacaagtaa aa
//
LOCUS       DAZKLM010000210         1164 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3764
            SAMEA115706913-rid22582563.denovo.219, whole genome shotgun
            sequence.
ACCESSION   DAZKLM010000210 DAZKLM010000000
VERSION     DAZKLM010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706913
            Sequence Read Archive: ERR13196647
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1164)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1164)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287823.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287823.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,004
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,849
            CDSs (with protein)               :: 2,849
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 41 of 96
            Pseudo Genes (incomplete)         :: 52 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 15 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1164
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706913-rid22582563.denovo.219"
                     /isolate="VRE3764"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..271)
                     /locus_tag="ACG842_002832"
     CDS             complement(<1..271)
                     /locus_tag="ACG842_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5960 family protein"
                     /protein_id="HFQ0249294.1"
                     /translation="MLFNETQLWFKFDPSNRFVKDFYKVWDSEVFFLAIEDSLLINLY
                     YSNKNYFKIPAAKTRMKKDVYFLFDIVTDVLDARSDHRRYDYIKYT"
     gene            complement(277..507)
                     /locus_tag="ACG842_002833"
     CDS             complement(277..507)
                     /locus_tag="ACG842_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0249295.1"
                     /translation="MMEEFIRKNISDEYADFYEQSNEKDKFQMDVSILAILAFSENKQ
                     PVTAKKETVFSEGKIKTRYILEVETKFKNRSE"
     gene            complement(766..879)
                     /locus_tag="ACG842_002834"
                     /pseudo
     CDS             complement(766..879)
                     /locus_tag="ACG842_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315396.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
BASE COUNT          414 a          191 c          170 g          389 t
ORIGIN      
        1 aagtatactt tatatagtca taacgccgat gatcgcttcg agcgtctagc acatcagtca
       61 cgatatcaaa caaaaaatat acgtctttct tcattctagt tttcgcagca gggattttaa
      121 agtagttctt attagaatag tagagattaa ttaataagct atcttcgatt gctaaaaaga
      181 aaacttctga atcccacacc ttataaaaat ctttgacaaa tctattcgaa ggatcaaatt
      241 taaaccataa ttgcgtctca ttaaaaagca taaccattac tccgatctat ttttaaactt
      301 agtctctacc tctaatatat atcgagtttt tattttgcct tcagagaata ctgtttcttt
      361 ttttgcagtt acaggttgtt tattttcgga aaaagctaat atagctaaaa ttgaaacatc
      421 catctggaat ttgtcttttt cgttgctttg ctcataaaaa tctgcatatt catcactaat
      481 attttttcta ataaattctt ccatcataaa gatcacctcg aaaagagtat acgaacaaac
      541 gttctttttg taaaggtgga atttattata ctaaataaat aggtgaacaa gtacttgtgc
      601 caaattatgt gccaaaaaaa atcgaattta ataaaacaca aacaaaaaga aatcctatta
      661 tgataacatt tcttataata acaaacacaa cagaagacgt gtaatagtta gtcaggaacg
      721 tacaaataac ccctgtatcc tttgcggtac agggggttat tttgtataag tagacattgt
      781 agtttctaaa ctgctatgtc ctaaacgttt cgatacgcta agtatattta ctccttgata
      841 aagtaaaata gatgcatgcg tatgtcttag tccatcaata aacagtcgaa gagaaaatag
      901 ggaaaaattg attactaaag aaaaacttac taggtcgacg gtgtagttga ttttcatact
      961 tcaatagatt cgaattaaac gatttcgatt ggcttatacc agtcctatcc aacgaaagat
     1021 atatcgttct taactctagc ggtataactt gaagctttac gtaaagccaa tgggaatgaa
     1081 ctggagatga ctttttatta tttttagtaa tagctgatgg gttaatactt acaactttcc
     1141 ttttcaaagt accatataaa aaaa
//
LOCUS       DAZKLM010000211         1140 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3764
            SAMEA115706913-rid22582563.denovo.221, whole genome shotgun
            sequence.
ACCESSION   DAZKLM010000211 DAZKLM010000000
VERSION     DAZKLM010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706913
            Sequence Read Archive: ERR13196647
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1140)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1140)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287823.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287823.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,004
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,849
            CDSs (with protein)               :: 2,849
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 41 of 96
            Pseudo Genes (incomplete)         :: 52 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 15 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1140
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706913-rid22582563.denovo.221"
                     /isolate="VRE3764"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..520)
                     /locus_tag="ACG842_002835"
     CDS             complement(<1..520)
                     /locus_tag="ACG842_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HFQ0249296.1"
                     /translation="MMKVIFFGLGSIGKRHLKNLISYGKKNSINFDITAYSSSGKKEF
                     EEITYIQNRDDLDTHYDIAFITNPTFKHLETLNVIVEKVDYIFLEKPAFSESIDLTQY
                     NYDYKNIYVAAPLRYKNIMEVVRNSIQSKKVYSVRAICSTYLPDWRKGTDYRNNYSAF
                     KSMGGGVELDCIH"
     gene            complement(520..>1140)
                     /locus_tag="ACG842_002836"
     CDS             complement(520..>1140)
                     /locus_tag="ACG842_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFQ0249297.1"
                     /translation="LKRKEIQYIKSKVTHNKKISYQYHVGQYLPDWHPWEDYRNFFVS
                     NKKTNGCREIFAIELPWIIDTFGKVKSFTVMTQKLSNLDIDYSDSFTLIIEHETGIVG
                     TLSVDVVSRVAKRNLTIIGEKILIEWEGNPDSLFSWNEETSSMTDIQLYDEVMHDNNY
                     SHTIIEDAYLEEIKEFFTLLEKKMEPQYTFEKDKYIINLINDIEVN"
BASE COUNT          385 a          201 c          126 g          428 t
ORIGIN      
        1 catgtataca gtctaattct actcctcccc ccatagattt aaaggcagaa taattatttc
       61 tataatcagt accttttctc caatcaggaa gataagtaga acaaatagct cttacagaat
      121 atactttttt actttgaata ctattacgaa caacttccat gatattttta tacctcagtg
      181 gagcagcaac ataaatattt ttgtaatcat aattatattg agtaagatca atactctcag
      241 aaaaagcagg tttttctaaa aatatgtaat caactttttc aacaattaca tttagtgttt
      301 ccagatgctt aaaagtcgga tttgtaataa aagctatatc ataatgtgta tccaaatcgt
      361 ctctattttg aatatacgtt atttcttcaa actctttttt tccactgcta ctataagctg
      421 ttatatcaaa attaatagaa ttcttttttc catagcttat tagatttttt agatgtctct
      481 ttccaattga ccctaaccca aaaaaaatta ctttcatcat taatttacct caatgtcgtt
      541 aattaagtta ataatatact tatctttttc aaaagtatat tggggttcca ttttcttttc
      601 caaaagcgtg aaaaattctt taatctcctc taaatatgca tcttcaataa tagtatgtga
      661 ataattatta tcatgcataa cctcatcgta caattgaata tctgtcatac tagaagtttc
      721 ttcattccat gaaaatagag aatccgggtt accttcccat tctattaaaa tcttttcacc
      781 aattatagtt aaatttcttt tagctactct tgaaacaaca tctacactta aagttccaac
      841 tataccagtt tcatgctcaa taataagagt gaaactgtcc gaataatcaa tatcaagatt
      901 ggataatttc tgggtcatta cagtaaacga tttaactttc ccaaatgtat ctataatcca
      961 aggtaattca atagcgaata tttctctaca cccattagtt tttttgtttg aaacaaaaaa
     1021 attgcgataa tcttcccaag gatgccagtc gggtaaatac tgtcctacat ggtattgata
     1081 agaaattttt ttattatgcg tcactttaga cttaatatat tgaatttctt ttctcttcaa
//
LOCUS       DAZKLM010000212         1139 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3764
            SAMEA115706913-rid22582563.denovo.222, whole genome shotgun
            sequence.
ACCESSION   DAZKLM010000212 DAZKLM010000000
VERSION     DAZKLM010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706913
            Sequence Read Archive: ERR13196647
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1139)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1139)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287823.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287823.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,004
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,849
            CDSs (with protein)               :: 2,849
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 41 of 96
            Pseudo Genes (incomplete)         :: 52 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 15 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1139
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706913-rid22582563.denovo.222"
                     /isolate="VRE3764"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(189..262)
                     /locus_tag="ACG842_002837"
     tRNA            complement(189..262)
                     /locus_tag="ACG842_002837"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(226..228),aa:Ile,seq:cat)
     gene            complement(817..1131)
                     /locus_tag="ACG842_002838"
     CDS             complement(817..1131)
                     /locus_tag="ACG842_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0249298.1"
                     /translation="MDFYSNFILIIAILLLLNIWFFDKSRNAGIGFRTKRSTSSEKKW
                     VYSQTIFYGGVISISLLSSTLYSFNVIDVSMSNFISIIGILISAIITQLLLVFEEKSK
                     NN"
BASE COUNT          431 a          179 c          171 g          358 t
ORIGIN      
        1 ttccatttaa aaatcaaagt aaaacttttc aaatatacag aagtataact atgtgaaaca
       61 tcctttcatt aatccataaa aggatacata aaaaagccac tcatttgagt ggcaatgaag
      121 aaaagcttta gcttgtataa tactcttcaa aaaattctaa cacagaacga ttcaattggc
      181 tacattaatg taccctgtag gactcgaacc tacgaccgga cggttatgag ccgtctgctc
      241 tgaccaactg agctaagggt agtggttgtt gccacataaa gccataaaca atcaaccagt
      301 agaatgcgtg gcaacaaacc tgttatcgca tatcttggag tgtgactatt tatgagtgat
      361 agtgaagata tgcgtctcca tatatgtatg gttgagacag ataaacatgt atgaaaaacg
      421 ttgttaaatc aacgtttttt tattttggtc aaggagagaa aagtagagaa atacaaaatt
      481 ttttgcattg attttgcatt caaaaaaaca ttcaaacatt tagaaatccc caatacgtat
      541 tcaggaaaat ctattttaag gtttacaaaa agaacttttg ttcgtacact ctttttgagg
      601 tgaactttta tgctgatgga aggaaaaaca caattatggt ttaagcatgt tccttcaaat
      661 aagtttttta attataaact ctagagaaat taattcatcc taacataaaa taattgttca
      721 ctcaaaagtt tttctagaac aattaaaaca gcaggcttca cattcgctat acatttgtaa
      781 gaagtaataa ttcctgtact aaaaggcata attgctttaa ttattttttg acttctcttc
      841 aaacacaaga agtagttgtg taataattgc agaaataagg attccaataa tagatataaa
      901 atttgacatg gaaacgtcta taacattaaa agaataaagt gttgaagaaa gtaaactgat
      961 tgaaataacc cctccgtaaa aaatagtctg tgaatatacc cacttttttt cagaagacgt
     1021 acttcgtttg gttctaaaac ctatccctgc atttctactc ttatcaaaaa accaaatatt
     1081 tagaagtaat aatattgcta ttattaagat aaaattactg taaaaatcca aaagctatc
//
LOCUS       DAZKLM010000213         1137 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3764
            SAMEA115706913-rid22582563.denovo.223, whole genome shotgun
            sequence.
ACCESSION   DAZKLM010000213 DAZKLM010000000
VERSION     DAZKLM010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706913
            Sequence Read Archive: ERR13196647
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1137)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1137)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287823.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287823.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,004
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,849
            CDSs (with protein)               :: 2,849
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 41 of 96
            Pseudo Genes (incomplete)         :: 52 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 15 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1137
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706913-rid22582563.denovo.223"
                     /isolate="VRE3764"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..355
                     /locus_tag="ACG842_002839"
     CDS             <1..355
                     /locus_tag="ACG842_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0249299.1"
                     /translation="GAPIRKFDKVYSTKHPLKLSTLLNATSRVLVEKYETNMTRDELL
                     RKALDEYIKQNLTQEDKQDLFNDVVKELDLFREKYPIVPELDDRGEIIRTTSQIKQDT
                     EDSLRKGWGMNNKVR"
     gene            615..910
                     /locus_tag="ACG842_002840"
                     /pseudo
     CDS             615..910
                     /locus_tag="ACG842_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348804.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            985..>1137
                     /locus_tag="ACG842_002841"
     CDS             985..>1137
                     /locus_tag="ACG842_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HFQ0249300.1"
                     /translation="MNHFKGKQFKKDVIIVAVGYYLRYNLSYREVQELLYDRGINVCH
                     TTIYRWV"
BASE COUNT          431 a          135 c          237 g          334 t
ORIGIN      
        1 tggcgcacca atcagaaagt ttgataaggt ttattccacc aagcatcctt taaaactgtc
       61 gactttgtta aatgcgactt ccagagtttt agtagaaaag tatgagacta atatgacaag
      121 agatgaattg cttagaaaag ctttagatga gtatattaaa cagaatctta cgcaagaaga
      181 taagcaagat ttgtttaatg acgtggtgaa agaactagac ttgttcagag aaaaatatcc
      241 aattgttcca gaattagatg atcgtggaga aataataaga actaccagtc agataaaaca
      301 agataccgaa gactcactgc gcaaagggtg gggaatgaat aataaagtta gataataatc
      361 aaagaagaac taagtaacta cctttaaacg gtagttactt agttcttctt tgatttgcat
      421 gaaatagagg ggatttattt ttctggttac gtgattaaaa aatccaaaaa atgagcatag
      481 gaacggttat gattttaggg tgtgttttaa tgagatattg tgatttcatt aacaagtgta
      541 tgataatctg ttgttgagtt agtaaataag taatagagct aactctaaaa taaaactatt
      601 aggaggagat gtaggtgaaa aagtttaaaa agaaaatata tttaggattg gttgttgggg
      661 tgagtatgtt tgggttcatg gctcatacta aacaagtaac tgcagatact aaccaaaaag
      721 aaaggattat gagccaatta gatgactctc aaataagtat gtacccaatg agctaattgt
      781 cagtttaaag gctacacaaa gaatttcaac agagaaaaat aaaccgcaaa atttattggg
      841 tgtaaaagtt atggtggaaa aagatttgac tcttaggaca aatgaaagcg attgtaataa
      901 gcgagatcaa gggttctgtt gcaaagtttt ccaaaaaatc tattttagtg taaaattgag
      961 aaaaaagaca gagaggacag agtaatgaat cattttaaag gcaaacaatt caaaaaagac
     1021 gtcattattg tcgctgttgg ttactacctg cgttacaatc taagctatcg tgaagttcag
     1081 gaattgttat atgatcgtgg aataaatgtt tgtcatacta cgatttatcg ttgggta
//
LOCUS       DAZKLM010000214         1120 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3764
            SAMEA115706913-rid22582563.denovo.224, whole genome shotgun
            sequence.
ACCESSION   DAZKLM010000214 DAZKLM010000000
VERSION     DAZKLM010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706913
            Sequence Read Archive: ERR13196647
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1120)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1120)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287823.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287823.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,004
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,849
            CDSs (with protein)               :: 2,849
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 41 of 96
            Pseudo Genes (incomplete)         :: 52 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 15 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1120
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706913-rid22582563.denovo.224"
                     /isolate="VRE3764"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>1120
                     /locus_tag="ACG842_002842"
     CDS             <1..>1120
                     /locus_tag="ACG842_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HFQ0249301.1"
                     /translation="FIKGISFITIPIFVNITTQAEYGLINNFTSFVSIFSILVGLSLN
                     SAINNASFDYKDDINEFMSSVLSLATLSLIVFLLIGNIYFLFSNAFFDMNQLVFNLML
                     LQSFASFVISYIQSYFTINFNHFKYLILSFISTFLNIAISVALMQTLYFDDKYVGRVV
                     GGTLSFVLIGLVIYLRIMIKGAHFFDKKQWRYALKISLPLIPHSLANIVLAQFDRIMI
                     NSYLGPAAGGIYSYISNIGIILSVIWMSTNNAWVPWFYGEMSKNNEKKVKKVSNYYLV
                     VFTLITMIVMVVSIDLAKFMAPKDYYSGLSLVVPITLGYFFQFLYSLPVNAEFYEKKT
                     SFIAIGTVASAIVNILLNMIFIPRLGIIAAGYTTVFTYF"
BASE COUNT          341 a          142 c          184 g          453 t
ORIGIN      
        1 ttttattaag ggaatttcat tcataactat tcctattttt gtaaatatta ctactcaagc
       61 agaatatggt ttgattaata atttcacttc atttgtatca attttttcga ttcttgttgg
      121 gctaagccta aattcggcaa ttaataatgc aagttttgac tataaagatg acataaatga
      181 atttatgtca tcggtacttt ctctagctac actttcgtta attgtgttct tattaatagg
      241 gaacatatat tttttgtttt caaatgcttt ttttgatatg aaccaacttg tatttaattt
      301 gatgttatta caaagttttg ctagttttgt aattagttat atccagtctt attttacgat
      361 taactttaac cattttaaat atttgatttt atcttttata tctacttttt tgaatattgc
      421 aatctctgta gctttgatgc agacattata ttttgatgat aaatatgttg gcagagtagt
      481 cggcggaaca ctgtcatttg tgttgatagg tttagtaatt tatttaagaa taatgataaa
      541 gggtgctcat ttttttgata aaaaacagtg gagatacgct ttaaaaatct ctttgccgct
      601 aatcccacat tccttagcaa atatagtttt agcacaattc gatagaatta tgattaattc
      661 ttatttaggt cctgctgcgg ggggaatata ttcctacatt agtaacatag gtattattct
      721 aagcgtaata tggatgtcta ctaataacgc ctgggttcca tggttctatg gtgagatgag
      781 taaaaataat gaaaaaaaag tgaagaaagt tagtaactat tatttagtag tctttacttt
      841 gattacgatg attgtaatgg tagtttcgat tgatttagca aaatttatgg ctccgaaaga
      901 ctattattct gggttatctt tagtggttcc gataacctta ggctatttct ttcagttttt
      961 atatagttta ccagttaatg ctgaatttta tgagaaaaaa acatcgttta ttgcgattgg
     1021 tacagtagca agtgctatag ttaatatact attaaacatg attttcatac ctaggttagg
     1081 aataattgct gctggataca caaccgtatt tacgtatttt
//
LOCUS       DAZKLM010000215         1112 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3764
            SAMEA115706913-rid22582563.denovo.225, whole genome shotgun
            sequence.
ACCESSION   DAZKLM010000215 DAZKLM010000000
VERSION     DAZKLM010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706913
            Sequence Read Archive: ERR13196647
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1112)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1112)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287823.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287823.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,004
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,849
            CDSs (with protein)               :: 2,849
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 41 of 96
            Pseudo Genes (incomplete)         :: 52 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 15 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1112
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706913-rid22582563.denovo.225"
                     /isolate="VRE3764"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(267..971)
                     /locus_tag="ACG842_002843"
     CDS             complement(267..971)
                     /locus_tag="ACG842_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0249302.1"
                     /translation="MKKKQKQKLLNQFRPSLDGVRGQLFRSLEEESLRRYGLPLQVML
                     DPKNRQILTIGLAGKTNEDTRINVPIDDNFNTVLKRIRSGENGIFERFRDNLLIEIVS
                     YWNDQRLKNDEPTAQAVATPVTEETVSVPEEKTEKPKADTTENTRSTDTNAALSFADF
                     SKAVAEYPKFYTEQNEHEVVINEKNADEPRKLATISMTTENEFVIEKALERKYKVKLT
                     LIPLIEQFAATAITDR"
BASE COUNT          321 a          194 c          199 g          398 t
ORIGIN      
        1 cttttttctc tatcagaaaa tatgtttatt attagaattt tttatagtta aggttaggca
       61 tcttaagaaa tcaggcaact ccaagatatc ttcaggtaga agagaagtcg cctaacattg
      121 taggatcata ggtgatttgt tggacaggtc aagctgatat ttgagcctga tgttagaagg
      181 aatttctttt tacttgctgt agaaattatt atatgactaa aaaggctggg attttaaatc
      241 ccagcctttt atgacaaata gaatttttat cgatcagtaa tcgctgtcgc tgcaaattgt
      301 tcgatcaaag gaatcaaagt caatttgact ttgtatttcc gttcgagtgc tttttcgata
      361 acgaactcat tttcagtagt catagaaata gttgctaatt tacgaggttc atcagcattt
      421 ttttcattaa taactacttc atgttcattt tgttctgtgt aaaatttagg atactcagca
      481 acggcttttg aaaaatcagc aaaacttaaa gcggcattcg tatcagttga gcgtgtattt
      541 tcagttgtgt ccgcttttgg tttttccgtt ttttcttctg gcacactgac tgtttcttct
      601 gtaactggtg tcgcaacagc ttgcgcagtt ggttcatcgt ttttcaatcg ttgatcattc
      661 cagtaagaca cgatttcaat aagaagattg tcacggaatc gttcgaaaat cccgttttct
      721 cctgaacgaa ttctctttaa tacagtattg aagttgtcat cgattggcac gttgatccgt
      781 gtatcttcat ttgttttccc agctaatccg atcgttaaga tttgacggtt tttgggatca
      841 agcatgactt gtaacggcaa accataacgt cgcaagctct cttcttctag agaacggaat
      901 aattgtcctc ttacaccatc caatgaagga cggaattgat ttaataattt ttgtttctgt
      961 tttttcttca tatgttctct ccttcgctca taccctttcg attataagaa aaaaacagcc
     1021 aaatgaaaag gagtaatgca aaaaaacttg cgtatacgca aattacaaca gaaaaatttg
     1081 cgtattttcc cgttttttga aagaaaaata aa
//
LOCUS       DAZKLM010000216         1073 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3764
            SAMEA115706913-rid22582563.denovo.226, whole genome shotgun
            sequence.
ACCESSION   DAZKLM010000216 DAZKLM010000000
VERSION     DAZKLM010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706913
            Sequence Read Archive: ERR13196647
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1073)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1073)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287823.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287823.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,004
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,849
            CDSs (with protein)               :: 2,849
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 41 of 96
            Pseudo Genes (incomplete)         :: 52 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 15 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1073
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706913-rid22582563.denovo.226"
                     /isolate="VRE3764"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>1073
                     /locus_tag="ACG842_002844"
     rRNA            <1..>1073
                     /locus_tag="ACG842_002844"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          329 a          214 c          308 g          222 t
ORIGIN      
        1 attgtgtgaa tacatagcac attcgaggta gacgcagaga actgaaacat ctaagtacct
       61 gcaggaagag aaagaaaatt cgattccctg agtagcggcg agcgaaacgg gaaaagccca
      121 aaccagcaag cttgcttgtt ggggttgtag gactccaata tggtagttct ttcagatagt
      181 cgaatgactt ggaaaagtca gtcaaagagg gtaaaaaccc cgtagacgaa atgtggaaga
      241 cacctaggag gatcctgagt acggcggaac acgagaaatt ccgtcggaat ccgggaggac
      301 catctcccaa ggctaaatac tccctagtga ccgatagtga accagtaccg tgagggaaag
      361 gtgaaaagca ccccggaagg ggagtgaaat agaacctgaa accgtgtgcc tacaacaagt
      421 caaagcccgt taatgggtga tggcgtgcct tttgtagaat gaaccggcga gttacgattg
      481 catgcgaggt taagttgaag agacggagcc gcagcgaaag cgagtctgaa tagggcgttt
      541 gagtatgtag tcgtagaccc gaaaccatgt gatctaccca tgtccaggtt gaaggtgcgg
      601 taaaacgcac tggaggaccg aacccacgta cgttgaaaag tgcggggatg aggtgtgggt
      661 agcggagaaa ttccaaacga acttggagat agctggttct ctccgaaata gctttagggc
      721 tagcctcgga attgagaatg atggaggtag agcactgttt ggactagggg cccatctcgg
      781 gttaccgaat tcagataaac tccgaatgcc attcattcat atccgggagt cagactgtga
      841 gtgataagat ccatagtcga aagggaaaca gcccagacca ccagctaagg tcccaaaata
      901 tatgttaagt ggaaaaggat gtggggttgc acagacaact aggatgttgg cttagaagca
      961 gccaccattt aaagagtgcg taatagctca ctagtcgagt gaccctgcgc cgaaaatgta
     1021 ccggggctaa acatattacc gaagctgtgg agtacacctt taggtgtatt ggt
//
LOCUS       DAZKLM010000217         1042 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3764
            SAMEA115706913-rid22582563.denovo.227, whole genome shotgun
            sequence.
ACCESSION   DAZKLM010000217 DAZKLM010000000
VERSION     DAZKLM010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706913
            Sequence Read Archive: ERR13196647
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1042)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1042)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287823.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287823.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,004
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,849
            CDSs (with protein)               :: 2,849
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 41 of 96
            Pseudo Genes (incomplete)         :: 52 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 15 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1042
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706913-rid22582563.denovo.227"
                     /isolate="VRE3764"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            144..>1042
                     /locus_tag="ACG842_002845"
     CDS             144..>1042
                     /locus_tag="ACG842_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HFQ0249303.1"
                     /translation="MLKEKMKTLPMLPGVYLMKDRDGKVIYVGKAKRLKNRVSSYFHQ
                     NKQHSKKVLRMIHHITDFDFVVVDTELDALLLECQLIQHYRPFYNRQMNYFSNYNYVH
                     ITNKGFVLTDTPTARTYGPFRLYKKMPSILRIMEETYQMPWLSEISLLALRVQLPDLQ
                     EMTFEQKKKELQGLFQGRNKKLLTYLKKRQQHFIYQLNFEKAGMLQKDIELVTYFIRR
                     IQEQKQFLRTPSLTFSMPLAADESQKKHYLVCYGQLAETMITSGDVSPDFYYEKKEAH
                     LSLKRQLSKEEIDPVQILISYRK"
BASE COUNT          365 a          186 c          172 g          319 t
ORIGIN      
        1 tcttttatta gaaaaatcat ctatctcaca tattttgatt atcaaagtat tttctgtgaa
       61 cagaactata gaattcccta aaacttgtcc attatttcaa aatttgttat agtcttttta
      121 ttcaaagcgt aaaaggagat cctatgttaa aagaaaaaat gaaaactctc cccatgctgc
      181 cgggtgtcta tcttatgaaa gaccgagatg gtaaggtcat ctatgttggt aaagcaaaac
      241 ggctcaaaaa tagagtaagc agctattttc atcagaataa acagcattca aaaaaagtat
      301 tacgtatgat tcatcatata acagattttg atttcgtcgt agtagacacg gaattagatg
      361 ctttattgct cgagtgtcaa ttgatccaac attaccgtcc tttttataac cgacaaatga
      421 attatttcag taattataat tatgtacata ttaccaataa gggatttgta ttaacagata
      481 ctcctacggc tcgaacatac gggccttttc gtctctataa aaaaatgccg tctattttgc
      541 gtataatgga agaaacttat caaatgcctt ggttgtctga aattagtttg ttagctttgc
      601 gagtccagct tcctgaccta caggaaatga cttttgaaca gaaaaaaaag gaactccaag
      661 ggctgtttca aggccgaaat aaaaagctac tgacttactt gaaaaaaaga caacaacatt
      721 tcatctatca gttgaatttc gaaaaagccg gtatgcttca aaaagatatc gaattagtta
      781 cttattttat tagacggatc caggaacaaa agcagttttt aagaacacca tcgctcacct
      841 tcagcatgcc attagcagca gatgagagtc agaaaaagca ctacctcgtt tgttatggtc
      901 aacttgcaga aacaatgatt acctctggtg acgtttctcc tgacttctat tatgaaaaga
      961 aagaagctca tttatctttg aaacgtcaat tgtcaaaaga agaaatcgat cctgtacaaa
     1021 tcttgatcag ttaccgcaaa aa
//
LOCUS       DAZKLM010000218         1004 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3764
            SAMEA115706913-rid22582563.denovo.228, whole genome shotgun
            sequence.
ACCESSION   DAZKLM010000218 DAZKLM010000000
VERSION     DAZKLM010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706913
            Sequence Read Archive: ERR13196647
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1004)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1004)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287823.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287823.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,004
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,849
            CDSs (with protein)               :: 2,849
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 41 of 96
            Pseudo Genes (incomplete)         :: 52 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 15 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1004
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706913-rid22582563.denovo.228"
                     /isolate="VRE3764"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..623)
                     /locus_tag="ACG842_002846"
     CDS             complement(<1..623)
                     /locus_tag="ACG842_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFQ0249304.1"
                     /translation="MNSSDLISVIIPVYNVEKYVGSCLESVCNQTYKNLDIIIVDDGS
                     KDSSNKICEDYALKDSRIRIIHKNNAGLGLARNTGLEHVKGKYVAFIDSDDFVAANYI
                     EAMLNGIKRSNADTCYAGYYEYYDDNNIISKPARYDSTIFTNNAIIEKVLLGMIGTEP
                     SYKVDFILPMSVWHALYSSEIIQQHNIKFPSERDYISEDIIFHISYL"
BASE COUNT          336 a          194 c          104 g          370 t
ORIGIN      
        1 tgcaaatatg aaatatggaa tatgatatct tcagatatat aatccctttc tgacggaaac
       61 ttgatattgt gctgttgaat tatctcagat gaatacagcg catgccaaac tgacattggc
      121 aatataaaat cgactttata actcggctca gtgccaatca ttccaagcaa cactttttca
      181 attatagcgt tatttgtaaa tatcgtacta tcatatcttg ctggctttga aataatatta
      241 ttatcatcat aatattcata atatccagca taacatgtgt ctgcattact cctttttata
      301 ccatttaaca ttgcttcaat ataattagct gcaacaaaat catcagaatc aataaaagca
      361 acatattttc cttttacatg ttcaagtcct gtatttcttg ccaatcccaa gccagcatta
      421 tttttatgaa taatacgaat ccttgaatcc tttaatgcat agtcttcaca tattttatta
      481 gatgaatcct tagatccatc atccacaatt ataatatcta ggttcttata tgtttgatta
      541 cacactgatt ccaaacaact tcctacatac ttttcaacat tatatacagg tatgattaca
      601 ctaattagat cactcgaatt catccttcta tcctttcact cacgttttct caattagtca
      661 atgacggcat caacgatttt cttaaattct ctgaaaaaaa tagactctga tcttgatgat
      721 gtgaatgaca tactatcttc ttctagaaaa attatttcct ttaaattatt tattaaatca
      781 ataatatgat cctcgtaagt atgctttccc ttggaataat gaaaatatac ttttgtatta
      841 cacaatattg caattttatt tcttaatatg attatttcac tcattcatta ctgccacata
      901 tacacaactt ataaccaccc cctgcaaagt ttagggtaag ttatcagata atatcttcct
      961 cttcatcatc cagaatacaa aatctattta tttatagaaa tatc
//
LOCUS       DAZKLM010000219         1002 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3764
            SAMEA115706913-rid22582563.denovo.229, whole genome shotgun
            sequence.
ACCESSION   DAZKLM010000219 DAZKLM010000000
VERSION     DAZKLM010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706913
            Sequence Read Archive: ERR13196647
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1002)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1002)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287823.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287823.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,004
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,849
            CDSs (with protein)               :: 2,849
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 41 of 96
            Pseudo Genes (incomplete)         :: 52 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 15 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1002
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706913-rid22582563.denovo.229"
                     /isolate="VRE3764"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            369..1002
                     /locus_tag="ACG842_002847"
                     /pseudo
     CDS             369..1002
                     /locus_tag="ACG842_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
BASE COUNT          342 a          182 c          183 g          295 t
ORIGIN      
        1 tttcaaagtt agtagagaaa atgtagttat atatttaaat ttttaccaga aagaaatgaa
       61 caccttcaat tatctaaatc tcgaaaatgg ttagatgtaa tcatcaggaa aaacaatctt
      121 aaacgaatta ctattcatgg tcttagacac acacatgcta gcttgctttt agcaagctag
      181 tgcaaatatt aaagatgtgc aagagcgttt aggccactct tctattcaaa tcattatgga
      241 tctatatatt catattacag acaaaagaaa agaagaaacc gtagcgcaat tcgcaaaata
      301 taggctctat aatttatggg actgatgaat caaaattgat tcttcagtcc cattgttatt
      361 ttaggtatat gattcgtaca ttgaaagagt tgaaaaacta tcttccgaag ccgtcaaaaa
      421 ggaccctgcc aaaagtattg atggtggatg aatttcgttc gcacgcttca attgtggata
      481 aaatgagctt tatttgtgcg gatggcgaga ctggaaaact cattgatatt ttgcctacac
      541 gaaaattgtc tcgattaaca cactatttta gtcgctgtgc aaatcctgaa gcggtggaat
      601 atttagtgac agatatgaac gccgcctact tccaactgac aaaacgtgtc ttaccaaatg
      661 cgaaagtcgt cattgattgt tttcatatca tcaaacattt gaatcaggcg ttcaatgatt
      721 ttcgtgtaca agaaatgaaa agattacacc agttagggca acgacgggaa gccgagaaag
      781 tgaaaaagaa ctggcgcttt cttctaaaaa accgtcaaaa aatcaatcac tatgattaca
      841 aaacatggcg cagttttcgt gcacctaaat acccgctact cactgaagca atgatgattg
      901 atcgattgct ggcattttca acgcctttaa aggacgccta tacttttttc catgagctga
      961 ccgaagcctt tagagacaag gaatcagact tattttttac ct
//
LOCUS       DAZKLM010000220          991 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3764
            SAMEA115706913-rid22582563.denovo.230, whole genome shotgun
            sequence.
ACCESSION   DAZKLM010000220 DAZKLM010000000
VERSION     DAZKLM010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706913
            Sequence Read Archive: ERR13196647
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 991)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 991)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287823.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287823.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,004
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,849
            CDSs (with protein)               :: 2,849
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 41 of 96
            Pseudo Genes (incomplete)         :: 52 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 15 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..991
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706913-rid22582563.denovo.230"
                     /isolate="VRE3764"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(21..94)
                     /locus_tag="ACG842_002848"
     tRNA            complement(21..94)
                     /locus_tag="ACG842_002848"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(58..60),aa:Ile,seq:cat)
     gene            complement(108..181)
                     /locus_tag="ACG842_002849"
     tRNA            complement(108..181)
                     /locus_tag="ACG842_002849"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(145..147),aa:Met,seq:cat)
     gene            complement(209..281)
                     /locus_tag="ACG842_002850"
     tRNA            complement(209..281)
                     /locus_tag="ACG842_002850"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(246..248),aa:Ala,seq:tgc)
     gene            complement(301..374)
                     /locus_tag="ACG842_002851"
     tRNA            complement(301..374)
                     /locus_tag="ACG842_002851"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(338..340),aa:Pro,seq:tgg)
     gene            complement(384..457)
                     /locus_tag="ACG842_002852"
     tRNA            complement(384..457)
                     /locus_tag="ACG842_002852"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(421..423),aa:Arg,seq:acg)
     gene            complement(468..553)
                     /locus_tag="ACG842_002853"
     tRNA            complement(468..553)
                     /locus_tag="ACG842_002853"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(517..519),aa:Leu,seq:taa)
     gene            complement(570..641)
                     /locus_tag="ACG842_002854"
     tRNA            complement(570..641)
                     /locus_tag="ACG842_002854"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(607..609),aa:Gly,seq:gcc)
     gene            complement(653..725)
                     /locus_tag="ACG842_002855"
     tRNA            complement(653..725)
                     /locus_tag="ACG842_002855"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(690..692),aa:Thr,seq:tgt)
     gene            complement(743..824)
                     /locus_tag="ACG842_002856"
     tRNA            complement(743..824)
                     /locus_tag="ACG842_002856"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(788..790),aa:Leu,seq:tag)
     gene            complement(832..904)
                     /locus_tag="ACG842_002857"
     tRNA            complement(832..904)
                     /locus_tag="ACG842_002857"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(869..871),aa:Lys,seq:ttt)
     gene            complement(908..980)
                     /locus_tag="ACG842_002858"
     tRNA            complement(908..980)
                     /locus_tag="ACG842_002858"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(945..947),aa:Val,seq:tac)
BASE COUNT          260 a          283 c          235 g          213 t
ORIGIN      
        1 tgaaacttac aacgccacaa tggaccttgt aggactcgaa cctacgaccg gacggttatg
       61 agccgtctgc tctaaccaac tgagctaaag gtccaggctc ttaaaaatcg cggcggaggg
      121 gatcgaaccc ccgacctccc gggtatgaac cggacgctct agccagctga gctacaccgc
      181 gaaaaagata ttcattattt aaatgaaatg gagcctagcg ggatcgaacc gctgacctcc
      241 tgcgtgcaaa gcaggcgctc tcccagctga gctaaggccc cgtgtttaaa tttataatta
      301 tcgggaagac aggattcgaa cctgcgaccc cttggtccca aaccaagtgc tctaccaagc
      361 tgagctactt cccgtttaat taacgcgccc aagaggagtc gaacccctaa ccttttgatc
      421 cgtagtcaaa cactctatcc agttgagcta tgggcgcata tttgaaatgc cgaggaccgg
      481 aatcgaaccg gtacggtgat cactcaccgc aggattttaa gtcctgtgcg tctgccagtt
      541 ccgccacccc ggcgcgattg ctttggcaaa gcggaaaacg gggttcgaac ccgcgacccc
      601 caccttggca aggtggtgct ctaccactga gctatttccg cgtgtttaaa gatgccggct
      661 aaaggacttg aaccctcgac cctctgatta caaatcagat gctctaccaa ctgagctaag
      721 ccggcatgta aaacttaaat tatgcgggtg aagggacttg aacccccacg ccgtaaggcg
      781 ctagatccta aatctagtgc gtctgccaat tccgccacac ccgcaaaaat atgagccgta
      841 cagggctcga acctgtgacc ctctgattaa aagtcagatg ctctaccaac tgagctaacg
      901 gctcatatgg aggttaacgg gatcgaaccg ctgaccctct gcttgtaagg cagatgctct
      961 cccagctgag ctaaacctcc atggataact t
//
LOCUS       DAZKLM010000221          961 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3764
            SAMEA115706913-rid22582563.denovo.231, whole genome shotgun
            sequence.
ACCESSION   DAZKLM010000221 DAZKLM010000000
VERSION     DAZKLM010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706913
            Sequence Read Archive: ERR13196647
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 961)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 961)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287823.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287823.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,004
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,849
            CDSs (with protein)               :: 2,849
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 41 of 96
            Pseudo Genes (incomplete)         :: 52 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 15 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..961
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706913-rid22582563.denovo.231"
                     /isolate="VRE3764"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..459
                     /locus_tag="ACG842_002859"
     CDS             <1..459
                     /locus_tag="ACG842_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0249305.1"
                     /translation="AKYLKTHVEAIDGKEALVQTDGDQAIAGHKNFSGSVTINNKRVL
                     TTDDNRYEVVNLVVTNGNTGTAKLYREGKTVSIYFVALNGKSSGGNDSVILTVPEGYR
                     TPISFEQLVGSIDRSTLNSAQLSIGADGAIKWRRNSSYGSAYSFVITYSI"
     gene            473..904
                     /locus_tag="ACG842_002860"
     CDS             473..904
                     /locus_tag="ACG842_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0249306.1"
                     /translation="MKVVYKSIKPYGFEQIILNNQENIPENCTEIKPPVPNWRPRFDF
                     DKKQWVELATEEEKKGNAVDSVDELANIKALYETLKAENDELKQLNSKAMLNNVAIKQ
                     ENVLLKEKSESLAQLNSKTMLASVQNTKEIEEIKKQLQGGK"
BASE COUNT          362 a          140 c          203 g          256 t
ORIGIN      
        1 gctaaatact taaaaacgca tgtagaagct attgatggta aagaagcatt ggtgcagaca
       61 gatggagacc aagccattgc aggacataag aatttttcag gttctgtaac aatcaataat
      121 aaacgtgttt tgacgactga tgataacaga tatgaagtgg taaatctggt tgtcactaac
      181 ggtaatacag ggacagcaaa gctttatcgt gaaggaaaaa cagtttctat atattttgtg
      241 gctttaaacg gaaaaagtag tggcgggaat gattcggtta ttttaactgt tccagaaggc
      301 tatcggacac caattagttt tgaacaactg gttgggtcaa tagaccgttc tactttgaac
      361 agtgctcaac tatctattgg cgcagacgga gccattaaat ggagaagaaa ttcaagttat
      421 ggatcagctt attcatttgt tatcacttat tcaatttaag ggaggaaatc taatgaaagt
      481 agtttacaaa tcaatcaagc cttacggatt cgagcaaatc attttgaaca atcaagaaaa
      541 tatccctgaa aactgtacag agattaaacc accagttcct aactggagac caagatttga
      601 ttttgataaa aaacagtggg ttgagttagc tactgaagaa gagaaaaaag ggaatgctgt
      661 tgatagcgta gatgaattgg ctaatattaa ggcattatac gaaacgctga aggcagaaaa
      721 tgatgaattg aaacagctaa attctaaagc aatgctaaac aatgtagcaa tcaagcaaga
      781 aaatgtctta ttgaaagaaa agtcagaaag tttagcgcag ttgaattcaa aaacgatgct
      841 tgcttctgta caaaatacca aggaaatcga agaaatcaaa aaacaattac aaggtgggaa
      901 gtaacatgta ttcatatgaa gatatcaaac taatgtatga ctggggctgt ttcactaacg
      961 a
//
LOCUS       DAZKLM010000222          957 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3764
            SAMEA115706913-rid22582563.denovo.232, whole genome shotgun
            sequence.
ACCESSION   DAZKLM010000222 DAZKLM010000000
VERSION     DAZKLM010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706913
            Sequence Read Archive: ERR13196647
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 957)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 957)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287823.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287823.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,004
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,849
            CDSs (with protein)               :: 2,849
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 41 of 96
            Pseudo Genes (incomplete)         :: 52 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 15 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..957
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706913-rid22582563.denovo.232"
                     /isolate="VRE3764"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..878
                     /locus_tag="ACG842_002861"
     CDS             <1..878
                     /locus_tag="ACG842_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HFQ0249307.1"
                     /translation="SLNQPLPEVVCFDEFKSVKNVSGAMSFVMMDGKTHQLIDIVENR
                     QLNPLRDYFLRYPRKVREQVRLVVSDFYTPYRTLVKECFPNARIVADRFHISQHIGRA
                     FTNHRIQVMKTFKKRDRRSKHLKKYWRLLQKNAWELKGQHRYWRPSFRDHLTEAEIVD
                     RLLSYDDSLKRGYEVYQDFLSAIRRQDVPEFVALLKEDYKELPEHYQPVFTTFKKYRT
                     EIKRALRVPYSNGPIECLNNHIKVLKRIAYGFRNFQNYQERIFLYRGKYFKKTKNTTQ
                     LTKARTTTRLDKIAV"
BASE COUNT          300 a          212 c          191 g          254 t
ORIGIN      
        1 tttctttgaa ccagccgtta ccagaagtgg tgtgtttcga tgaattcaaa tcggtgaaaa
       61 acgtctcggg agccatgagc tttgtcatga tggacggcaa gacccatcaa ctgattgata
      121 ttgtggaaaa tcggcaacta aatcctttac gcgactattt cttgcgctac cctagaaaag
      181 tccgtgaaca agtacgtcta gtggtttcag atttctatac accctatcgc acattggtga
      241 aagagtgctt tccgaatgcc cgaatcgtcg ccgatcgctt tcatattagc caacacatcg
      301 gtcgggcctt taccaaccac cgaattcaag taatgaagac ctttaaaaaa agggatcgtc
      361 ggtcgaagca cttaaagaaa tactggagac tacttcaaaa gaatgcgtgg gaattgaaag
      421 gtcagcatcg ctactggcgt ccgtcatttc gagatcatct gacagaagca gagatagtcg
      481 atcggctatt atcttatgat gactccttaa agcgaggcta cgaagtctac caggattttc
      541 tttccgctat cagaagacaa gatgttcctg aattcgtcgc gctcctaaaa gaagactaca
      601 aggagttgcc agagcactac caaccagtgt tcacgacctt caagaaatat cgaacagaaa
      661 tcaaacgagc gttgcgagtt ccctattcca acggccctat agaatgtttg aacaaccata
      721 tcaaagtact aaaacgaatt gcctatggct ttagaaactt tcaaaactac caagaacgaa
      781 tctttttata tcgaggaaaa tattttaaga aaactaagaa cactacccaa ctgactaaag
      841 ctagaacaac aacacggcta gacaagatag ctgtctagcc gtgtgagtat ctcgattatt
      901 ctattgaatt tcggctctat actttttgat gttggtgaac ttcaccaaca tcttttt
//
LOCUS       DAZKLM010000223          929 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3764
            SAMEA115706913-rid22582563.denovo.233, whole genome shotgun
            sequence.
ACCESSION   DAZKLM010000223 DAZKLM010000000
VERSION     DAZKLM010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706913
            Sequence Read Archive: ERR13196647
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 929)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 929)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287823.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287823.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,004
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,849
            CDSs (with protein)               :: 2,849
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 41 of 96
            Pseudo Genes (incomplete)         :: 52 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 15 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..929
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706913-rid22582563.denovo.233"
                     /isolate="VRE3764"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>929
                     /locus_tag="ACG842_002862"
     CDS             <1..>929
                     /locus_tag="ACG842_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4041 domain-containing protein"
                     /protein_id="HFQ0249308.1"
                     /translation="NKLSLLKLKEKEYIIDDAVSVPLILNKREKNNLKKKMLIPFDSE
                     VKGLLNKLTISNIDSTREKIIKIFDKINNLFKNDNAELRKGLLEFKLQELELHYSYLV
                     KITEEKEQQKAIKEQMIEEEKVRREIDREKKRIDKEQRQFNSEISKLIAYMQKANADV
                     EKELYANKIQELEEKLKELEVIRENVLQRELNTRAGYVYVISNIGSFGEDIYKIGMTR
                     RLEPMDRIKELSSASVPFEFDVHAMIFSEDAPSLETKLHNHFRKQEVNKINQRKEFFK
                     VSLDEIEKVVLENYNGTVTFTKLAKAEQYRRSL"
BASE COUNT          405 a           99 c          154 g          271 t
ORIGIN      
        1 aaaataagct ttctttgtta aaattaaaag aaaaggaata tattattgac gatgctgttt
       61 ctgtccctct tattttaaat aaacgagaga aaaataattt aaagaaaaaa atgctcattc
      121 catttgattc agaagtgaaa ggattactca ataaactaac tatttctaat atagactcga
      181 ctagggaaaa aatcataaaa atatttgata aaattaataa tttattcaag aatgacaatg
      241 ctgaattaag gaagggtctt ttagagttta aacttcaaga actagaactt cattactctt
      301 atttagtgaa aattaccgaa gaaaaagaac aacaaaaagc aataaaagaa cagatgatag
      361 aagaggaaaa agttcgtaga gaaattgatc gtgaaaagaa acgtattgat aaagaacaac
      421 gtcaattcaa ttctgaaatt tccaaattaa tcgcatatat gcaaaaagct aatgcagatg
      481 tggaaaaaga attatatgca aataaaattc aagaactgga agaaaaatta aaagaattag
      541 aagttatacg tgaaaatgta ttacaaagag aattaaatac tagagccggt tatgtgtatg
      601 taattagtaa tattgggtct tttggtgaag acatatataa aattggtatg actagaagat
      661 tagaacctat ggatagaata aaagaattga gtagtgcatc ggttccattt gaatttgacg
      721 ttcatgcgat gattttttca gaggacgctc cgtctttgga aactaaattg cataatcatt
      781 ttagaaaaca agaagttaat aaaattaatc aaagaaaaga atttttcaaa gtttcactag
      841 acgaaataga gaaagttgtt ttagaaaatt ataatggaac agtaacattt acaaaattag
      901 ctaaagctga acaatataga cgatcttta
//
LOCUS       DAZKLM010000224          899 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3764
            SAMEA115706913-rid22582563.denovo.234, whole genome shotgun
            sequence.
ACCESSION   DAZKLM010000224 DAZKLM010000000
VERSION     DAZKLM010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706913
            Sequence Read Archive: ERR13196647
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 899)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 899)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287823.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287823.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,004
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,849
            CDSs (with protein)               :: 2,849
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 41 of 96
            Pseudo Genes (incomplete)         :: 52 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 15 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..899
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706913-rid22582563.denovo.234"
                     /isolate="VRE3764"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            256..879
                     /locus_tag="ACG842_002863"
     CDS             256..879
                     /locus_tag="ACG842_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFQ0249309.1"
                     /translation="MYLAYLRVSSDDQSLARQHKLIENWSVKNNIAEDDLKIFEEKVS
                     GKNIENRPELQELISFIRERDTIIIPSLDRLARNSKDTKDLLQLFRSKGASIEILDLP
                     SFNGVTDPALRDLLTNLVIEVFSYVAENERKKIKERQKQGIEIAKKNGRYTGKKIQYH
                     ANATGKNKLIYDEIVDGLKKKETISKIAKNAGVTRKTVYRVKRDLGM"
BASE COUNT          333 a          129 c          167 g          270 t
ORIGIN      
        1 aataaactaa tgtccttatt tttgtgttca catttctgtt acctaagaaa tacttaaagg
       61 aggtgatgta gccagcaacg actacaactt gaacccgcta tctacagttt atttgttgtt
      121 tcttttgggt atactctaat ggtacaaatc catatttgct taaagcgttg atttttcagc
      181 ggtttttctt tattgcaaag tgtaccctaa gacctgtata ctaatagtaa atagattgga
      241 taggaatgat ggcaaatgta tttagcttat ttaagagttt cttccgacga tcaaagttta
      301 gctcggcaac ataagctaat tgaaaattgg tcagtcaaaa ataatattgc tgaagacgat
      361 ttaaaaattt tcgaggaaaa agtctctgga aaaaatattg aaaacagacc agagctgcaa
      421 gagcttattt ctttcatacg tgaacgagat acgattatta ttcctagtct tgatcgatta
      481 gcaagaaata gtaaagatac aaaagactta cttcaattgt ttcgtagtaa aggtgcttct
      541 attgaaatat tagacctacc gtcgtttaat ggggtaactg accctgcatt aagagacttg
      601 ctaacaaatt tagtcataga agtgtttagc tatgttgctg aaaacgaacg gaaaaaaatc
      661 aaagagcgtc aaaaacaagg cattgaaatt gcaaagaaaa atggtcgtta tactggtaaa
      721 aagattcaat accatgccaa tgcaacagga aaaaacaaat tgatttacga tgaaattgtt
      781 gatggtctga agaaaaaaga aactatttct aaaatagcaa agaacgcagg agttacaaga
      841 aaaaccgttt atcgagtaaa aagagattta ggaatgtaat atgttccagt tagaaagaa
//
LOCUS       DAZKLM010000225          867 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3764
            SAMEA115706913-rid22582563.denovo.235, whole genome shotgun
            sequence.
ACCESSION   DAZKLM010000225 DAZKLM010000000
VERSION     DAZKLM010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706913
            Sequence Read Archive: ERR13196647
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 867)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 867)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287823.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287823.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,004
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,849
            CDSs (with protein)               :: 2,849
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 41 of 96
            Pseudo Genes (incomplete)         :: 52 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 15 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..867
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706913-rid22582563.denovo.235"
                     /isolate="VRE3764"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>867
                     /locus_tag="ACG842_002864"
     CDS             <1..>867
                     /locus_tag="ACG842_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0249310.1"
                     /translation="KVSGLQYDSLSEKTLKLEAGDIQVAFYQQQAVTIQDKLKDYATN
                     NYMSDFNDYVSSMITGQGSAGGYVVLWPKEKPSNIFIMDNPDLEKAREVLRMNKNGIA
                     FSKKGWRGPFNSAWTLDSIFNANFIQTGLIKADIFQNSFNKTGDVLKLVNGLLQIWNN
                     KKKIMELTKKGMEFWNSKGSIGTIGTTDSVGNPFPGASTPTPIEDNSLVIRTNGDGKY
                     ILISPKVGKGLVLLGNGKAIYFGDLDVQGKLTVKGKEIIPGQGGGPSGGGESTGGYPS
                     ELKTDAEKRAWR"
BASE COUNT          319 a          107 c          180 g          261 t
ORIGIN      
        1 aaaggttagt ggactacaat acgattcttt atctgaaaaa actttgaaat tagaagcagg
       61 agacattcaa gttgcttttt atcagcaaca agctgtaact attcaggata aattaaaaga
      121 ttatgcaacg aataattata tgagtgattt taatgattat gtttcttcaa tgattacagg
      181 tcaaggcagt gcaggaggat atgtagtttt atggccaaaa gaaaaacctt ccaacatctt
      241 tattatggat aatccagatt tagaaaaggc aagagaagta ctaagaatga ataaaaatgg
      301 gattgctttt tctaagaaag gatggcgggg gccttttaat tcagcttgga cattagatag
      361 tatatttaat gccaatttta tacaaactgg attaatcaaa gcagatatct ttcaaaattc
      421 ttttaacaag actggagatg tattgaaatt agttaatgga ctactccaaa tttggaacaa
      481 caaaaagaaa ataatggagt taactaaaaa aggaatggaa ttttggaatt ctaagggttc
      541 aattggaacg attggaacaa ctgattctgt tggtaatcct tttcctgggg cttctactcc
      601 aacccctatt gaagataatt ctttagttat tcgtacaaat ggagacggca aatatatttt
      661 gatttctcct aaagtaggaa aaggattagt tttattagga aatggtaaag caatttattt
      721 tggagactta gatgtacaag gaaaacttac agttaaagga aaagaaataa ttcctggaca
      781 aggtggtggt ccatcgggag gtggagaatc tactggtggg tatcctagtg aattaaaaac
      841 agatgcagaa aagagagctt ggagaat
//
LOCUS       DAZKLM010000226          853 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3764
            SAMEA115706913-rid22582563.denovo.236, whole genome shotgun
            sequence.
ACCESSION   DAZKLM010000226 DAZKLM010000000
VERSION     DAZKLM010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706913
            Sequence Read Archive: ERR13196647
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 853)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 853)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287823.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287823.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,004
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,849
            CDSs (with protein)               :: 2,849
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 41 of 96
            Pseudo Genes (incomplete)         :: 52 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 15 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..853
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706913-rid22582563.denovo.236"
                     /isolate="VRE3764"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(40..112)
                     /locus_tag="ACG842_002865"
     tRNA            complement(40..112)
                     /locus_tag="ACG842_002865"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(77..79),aa:His,seq:gtg)
     gene            complement(115..188)
                     /locus_tag="ACG842_002866"
     tRNA            complement(115..188)
                     /locus_tag="ACG842_002866"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(154..156),aa:Trp,seq:cca)
     gene            complement(195..275)
                     /locus_tag="ACG842_002867"
     tRNA            complement(195..275)
                     /locus_tag="ACG842_002867"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(239..241),aa:Tyr,seq:gta)
     gene            complement(277..352)
                     /locus_tag="ACG842_002868"
     tRNA            complement(277..352)
                     /locus_tag="ACG842_002868"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(317..319),aa:Phe,seq:gaa)
     gene            complement(388..460)
                     /locus_tag="ACG842_002869"
     tRNA            complement(388..460)
                     /locus_tag="ACG842_002869"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(425..427),aa:Asp,seq:gtc)
     gene            complement(470..542)
                     /locus_tag="ACG842_002870"
     tRNA            complement(470..542)
                     /locus_tag="ACG842_002870"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(507..509),aa:Val,seq:tac)
     gene            complement(558..629)
                     /locus_tag="ACG842_002871"
     tRNA            complement(558..629)
                     /locus_tag="ACG842_002871"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(594..596),aa:Glu,seq:ttc)
     gene            complement(644..733)
                     /locus_tag="ACG842_002872"
     tRNA            complement(644..733)
                     /locus_tag="ACG842_002872"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(697..699),aa:Ser,seq:gga)
     gene            complement(773..846)
                     /locus_tag="ACG842_002873"
     tRNA            complement(773..846)
                     /locus_tag="ACG842_002873"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(810..812),aa:Asn,seq:gtt)
BASE COUNT          237 a          236 c          193 g          187 t
ORIGIN      
        1 ccttgcctta ccgcttggct ataccccaag aataaaaagg gcgaatgatg ggaatcgaac
       61 ccacgaatgc cggagccaca atccggtgcg ttaaccactt cgccacattc gccatggcag
      121 gggcagcagg aattgaaccc acactaacgg ttttggagac cgctgttcta cctttaaact
      181 atgcccctat aaaatggagg agagtggatt cgaaccactg aaccctaagg aacggattta
      241 cagtccgtcg cgtttagcca cttcgctact cctccatggt ggcgcgggac agaatcgaac
      301 tgccgacaca cggagcttca atccgttgct ctaccaactg agctaccgcg ccatatttta
      361 aaattattaa ttgaataaca agaacgacgg ttccgacggg aatcgaaccc gcgatctcct
      421 gcgtgacagg caggcatgtt aacccctaca ccacggaacc agttcattat ggaggttaac
      481 gggatcgaac cgctgaccct ctgcttgtaa ggcagatgct ctcccagctg agctaaacct
      541 ccaacattca attaaaatga cccgtacggg attcgaaccc gtgttaccgc cgtgaaaggg
      601 cggtgtctta accgcttgac caacgggcca ggtaaatttg ctacggagag taagggattc
      661 gaacccttga gacagtttgg caccatctac atgatttcca atcatgctcc ttcggcctct
      721 cggacaactc tccagagtga aagaagctca atactctttc tcaaaatgag aactccggca
      781 gtaggactcg aacctacgac atcatgatta acagtcatgc gctactacca actgagctat
      841 gccggaataa cat
//
LOCUS       DAZKLM010000227          846 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3764
            SAMEA115706913-rid22582563.denovo.237, whole genome shotgun
            sequence.
ACCESSION   DAZKLM010000227 DAZKLM010000000
VERSION     DAZKLM010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706913
            Sequence Read Archive: ERR13196647
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 846)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 846)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287823.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287823.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,004
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,849
            CDSs (with protein)               :: 2,849
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 41 of 96
            Pseudo Genes (incomplete)         :: 52 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 15 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..846
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706913-rid22582563.denovo.237"
                     /isolate="VRE3764"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>846
                     /locus_tag="ACG842_002874"
     CDS             <1..>846
                     /locus_tag="ACG842_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0249311.1"
                     /translation="SLLYLFPAFKNMEIFGFYDTAFHINRALSLESIFSSPINFETFR
                     SYGMQVNNFYPWLTLYPLFLLIKFTNLAIGYNLFLYIVTLITLFICHYVMYEITKKHV
                     TSSFFAIIYTTSSFRSVEIFLRGAMGELLAMSILPLILLGFIKLYDSKKESWVMLAIS
                     MTLLIYTHVLSVAMTMVMIIIALIIQFYRKKKIEIFLIIKLIKAAIVTLLLSLAFIGP
                     MIEQTLYQDLNRPYVDILQKRAIYLGKEFLIGSIDSELLSFGIGLTLLICLIILTFNF
                     KKLGI"
BASE COUNT          267 a          116 c          121 g          342 t
ORIGIN      
        1 ttagtttatt atatttattt ccagcattta aaaacatgga aatatttggc ttttatgata
       61 cggcatttca cattaatcgt gcgttaagtt tagaatctat ttttagctcc cctattaatt
      121 ttgaaacgtt tagatcatat ggcatgcaag taaataattt ttacccatgg ttaactctat
      181 atcctttatt tttattgatc aagtttacta atttagcaat aggctataac ctttttcttt
      241 atattgttac gctcattact ctatttatat gtcattatgt aatgtatgaa attaccaaaa
      301 aacatgttac tagctccttt ttcgctatta tatatacaac atcttctttt aggagtgtag
      361 aaatatttct tcgaggggca atgggggaat tactcgcaat gtcaatactt cctcttattt
      421 tactcgggtt tataaagctt tacgactcaa aaaaagaatc ttgggttatg ttggcgatta
      481 gcatgacttt attaatttac actcatgttc tttctgtagc tatgactatg gtaatgatta
      541 taattgcttt aattattcaa ttttatcgga aaaagaaaat agaaatcttc ttaattatta
      601 aattaataaa agctgctata gttactttac ttctttcact agcgtttata ggtcctatga
      661 ttgagcagac attgtatcaa gatctgaata gaccttatgt ggatatatta caaaaaagag
      721 caatttatct gggtaaagag tttttgattg gaagtataga ttcagaatta ctatcatttg
      781 gtattggctt aactttgctt atttgtttaa ttatactaac atttaacttt aaaaaattag
      841 gaatct
//
LOCUS       DAZKLM010000228          822 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3764
            SAMEA115706913-rid22582563.denovo.238, whole genome shotgun
            sequence.
ACCESSION   DAZKLM010000228 DAZKLM010000000
VERSION     DAZKLM010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706913
            Sequence Read Archive: ERR13196647
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 822)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 822)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287823.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287823.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,004
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,849
            CDSs (with protein)               :: 2,849
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 41 of 96
            Pseudo Genes (incomplete)         :: 52 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 15 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..822
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706913-rid22582563.denovo.238"
                     /isolate="VRE3764"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            140..313
                     /locus_tag="ACG842_002875"
     CDS             140..313
                     /locus_tag="ACG842_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0249312.1"
                     /translation="MMKRLYYSLIITIGYLIVSNLGNMVFGISKEFSWTTTLWESLFF
                     FIFVFLLQNYRKK"
BASE COUNT          277 a          131 c          103 g          311 t
ORIGIN      
        1 aattttttcg gagaattaaa aaatacaata aataacttta gttactttaa tgcgttctta
       61 ctttattttt atattatcat ttttcttatt cacttctttg gtttatacta ttcttataaa
      121 taaaaacgag gtatgtccta tgatgaaaag attgtactat tcattgatca taacgattgg
      181 ctatttgatt gtatcaaatc taggaaatat ggtatttggt atcagtaaag aattcagttg
      241 gactactaca ttatgggaat ctctattctt tttcatcttc gtatttttgt tacaaaatta
      301 tcgaaaaaaa taaacatgtg ccatctatct gttggcacat gttttaggat ttatggtact
      361 ttttattcgg caatccattt tatttttcta cttcttctta atacctctaa cacttttgat
      421 ttataaacta atccaatgca tctacttgcg cttattaagc tatttcagct agatagtacc
      481 accccatgcg tcaatgcttg attttccttt tgtataacat tgtataagca ataaaggaca
      541 aataagtggt gattacacca aaccattgaa taagaaaaat tctgatacgt aaataaacat
      601 gaacttgaaa aaattaatga atcggttagc tactacgtag ttgtcttttt tctcatacag
      661 ttctgttaac ttacacttct cttcgctaga taataaaaaa tcctccaact gtattgtcca
      721 atataatccc aggcatctaa actagcatta tagaagagac acacatagaa tctaaaataa
      781 ctggttttaa atgtttctta caatcaaaaa aagctgttaa at
//
LOCUS       DAZKLM010000229          822 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3764
            SAMEA115706913-rid22582563.denovo.239, whole genome shotgun
            sequence.
ACCESSION   DAZKLM010000229 DAZKLM010000000
VERSION     DAZKLM010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706913
            Sequence Read Archive: ERR13196647
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 822)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 822)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287823.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287823.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,004
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,849
            CDSs (with protein)               :: 2,849
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 41 of 96
            Pseudo Genes (incomplete)         :: 52 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 15 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..822
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706913-rid22582563.denovo.239"
                     /isolate="VRE3764"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..153)
                     /locus_tag="ACG842_002876"
     CDS             complement(<1..153)
                     /locus_tag="ACG842_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFQ0249313.1"
                     /translation="MSKRTRRTFSQEFKQQIVNLYLAGKPRVEIIREYELTASAFDKW
                     VKQSKTS"
     gene            231..809
                     /locus_tag="ACG842_002877"
                     /pseudo
     CDS             231..809
                     /locus_tag="ACG842_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330160.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
BASE COUNT          259 a          151 c          158 g          254 t
ORIGIN      
        1 actcgtttta gattgcttta cccatttgtc aaatgctgaa gccgttagtt catattctcg
       61 aatgatttct acacgtggct ttccagctaa gtaaagattg acgatttgtt gcttgaattc
      121 ttgtgaaaaa gttcttcgtg ttctcttaga cataaaaatt cctcctggta tgttttcttc
      181 tagtctacac accttaattt ttctgtctag ttaattgtag cctatccaca ggaatcaaat
      241 cgcatttatg ggtaacacaa aatatttggg gcgatcatac agatccttgc ggttatcttt
      301 ctgaaatggg tgtaagtaaa gaaaaactag cctatgattt ggctcatggt tttacggatg
      361 ataatccaac tacttcggat gataatccaa ctacttcgga ggataaacca gtaattgatc
      421 caactcgagc aggtgctgca aattctacgc tgacagatgg aacaaattac gcccacgttg
      481 atcagttcgg agaaatcgaa aacgcaaact tgcatgtagc tggatggcac attgctaatt
      541 ataaatacga gtatattttc attatggact acaatactgg aaaagaacta gctagagtaa
      601 gagctgatgg gatttataga ccagacgtaa atcaagctta taatacttta ggaaatgttg
      661 gctatcatgt atctttcaat atgcgtgatt tccctaataa gaaagtatac gtcatgatgc
      721 gtgcgacaaa cgatccagaa ggaaatacta agggtggagc acaagatttt catgacaagc
      781 gttggtattt aaatattcca caacgataaa aaattccccc ct
//
LOCUS       DAZKLM010000230          818 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3764
            SAMEA115706913-rid22582563.denovo.240, whole genome shotgun
            sequence.
ACCESSION   DAZKLM010000230 DAZKLM010000000
VERSION     DAZKLM010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706913
            Sequence Read Archive: ERR13196647
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 818)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 818)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287823.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287823.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,004
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,849
            CDSs (with protein)               :: 2,849
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 41 of 96
            Pseudo Genes (incomplete)         :: 52 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 15 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..818
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706913-rid22582563.denovo.240"
                     /isolate="VRE3764"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..351
                     /locus_tag="ACG842_002878"
     CDS             <1..351
                     /locus_tag="ACG842_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HFQ0249314.1"
                     /translation="SVKKQFGKYSNLEINSNDLATYILRYDDKIIEIHLDYFGRVPTR
                     KFQLFTLDECINVDLLNNKISFEENIVKEMPESANDMYEKELKYFFENVVTGKENWND
                     LYHANEVLKIAEEK"
     gene            352..>818
                     /locus_tag="ACG842_002879"
     CDS             352..>818
                     /locus_tag="ACG842_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HFQ0249315.1"
                     /translation="MEILFTICGRAGSKGFKNKNLKYLAGKPLVHFALAMIDIYQQKH
                     PEHNCTTCLNTDSNELVELTTKLNPSVKIVERKPELATDTVGKIEVIRDSFLQLERED
                     NIFDLIIDLDLTSPLREVSNLEELIDEHLKNLDKDVYFSVVSARRNPYFNMVK"
BASE COUNT          319 a           90 c          144 g          265 t
ORIGIN      
        1 tcagtaaaaa aacagtttgg taaatattcg aatcttgaaa ttaacagtaa tgatttagca
       61 acatatatat tgagatacga tgataaaatt atagaaattc atttagatta ttttggaaga
      121 gttcctacaa gaaaatttca attgtttact ttagatgaat gtatcaatgt tgacttatta
      181 aataataaaa taagttttga agaaaacatt gttaaagaaa tgcctgaatc tgctaatgat
      241 atgtatgaga aggagttgaa atatttcttt gaaaacgtag taacaggcaa agaaaactgg
      301 aatgatttat atcatgcaaa cgaagtattg aaaatagctg aggagaaata aatggaaatt
      361 ttatttacta tttgtggtag agctgggtct aaggggttta aaaataaaaa tttgaaatat
      421 ttggcaggaa agcccctagt ccatttcgct ttagcaatga tagatatcta tcaacaaaaa
      481 catcctgaac ataattgcac gacttgtttg aatacggaca gtaatgagtt agtagaattg
      541 acgactaagt taaatccgtc tgtgaaaatt gtagaacgta aacctgaatt agctacagat
      601 actgtaggga aaattgaagt gatacgtgat tcattcttac aactagaaag agaagataat
      661 atatttgatt taattataga tttagatttg acttcacctt tacgtgaagt ttctaatctt
      721 gaagaattaa tagatgaaca tctaaaaaat ttagataaag acgtctactt ttcagttgtt
      781 tctgcaagac gtaatcccta ttttaatatg gtaaaaag
//
LOCUS       DAZKLM010000231          752 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3764
            SAMEA115706913-rid22582563.denovo.242, whole genome shotgun
            sequence.
ACCESSION   DAZKLM010000231 DAZKLM010000000
VERSION     DAZKLM010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706913
            Sequence Read Archive: ERR13196647
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 752)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 752)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287823.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287823.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,004
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,849
            CDSs (with protein)               :: 2,849
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 41 of 96
            Pseudo Genes (incomplete)         :: 52 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 15 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..752
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706913-rid22582563.denovo.242"
                     /isolate="VRE3764"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            132..344
                     /locus_tag="ACG842_002880"
     CDS             132..344
                     /locus_tag="ACG842_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014834536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0249316.1"
                     /translation="MAVWQIQLKERGAYGLISLSDVLCRKEMPDLFSLDQIIAIIGAA
                     AVVITAVAKASDSILAWLKFKKEQKK"
     gene            506..>752
                     /locus_tag="ACG842_002881"
     CDS             506..>752
                     /locus_tag="ACG842_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000357244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
                     /protein_id="HFQ0249317.1"
                     /translation="MDKFNRLTLINQFEILKALNPNEEKYYAEKIEILTEGYEYHYSE
                     IFENISDPLPEEDSRFVLDILNMYRDITFSKNKLKDIN"
BASE COUNT          277 a          105 c          127 g          243 t
ORIGIN      
        1 aaaacactat atattccgaa aagtgagtat ctccattcat aaaataatca acatatttaa
       61 aaaatctaat tagctttttt agtcaatcta aagttcttta ttctatgata taatgttact
      121 gtagaaagtt tgtggcggtt tggcaaatcc aattgaagga gcgtggtgcc tatgggctta
      181 ttagcttatc ggacgtactc tgtagaaagg aaatgcctga tttgttctca ttggatcaaa
      241 tcattgctat tatcggagca gcagcagtag ttattactgc cgtagcgaaa gctagcgatt
      301 cgattttagc ttggttaaag ttcaaaaaag aacaaaaaaa atagagccgt ctcaactttt
      361 ggcgaggtag acgagctcta aagctttatt tacatgccat ccgctttgaa cggactacac
      421 tgggcatgtg acagcatgcc ctttcattta tattatatct cattctttta tataatgaaa
      481 gtaaataaat catggaggat aaaatatgga taaatttaat agattaacat taataaatca
      541 gtttgaaatt ttaaaagctt taaatcctaa cgaagaaaaa tactatgctg aaaaaataga
      601 aatactaaca gaaggttacg aatatcacta tagtgaaata tttgaaaata tttctgatcc
      661 attgccagaa gaagattcgc ggtttgttct agacatatta aatatgtata gagatattac
      721 ttttagtaaa aataaattaa aagatataaa tt
//
LOCUS       DAZKLM010000232          730 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3764
            SAMEA115706913-rid22582563.denovo.243, whole genome shotgun
            sequence.
ACCESSION   DAZKLM010000232 DAZKLM010000000
VERSION     DAZKLM010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706913
            Sequence Read Archive: ERR13196647
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 730)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 730)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287823.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287823.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,004
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,849
            CDSs (with protein)               :: 2,849
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 41 of 96
            Pseudo Genes (incomplete)         :: 52 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 15 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..730
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706913-rid22582563.denovo.243"
                     /isolate="VRE3764"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..215)
                     /locus_tag="ACG842_002882"
     CDS             complement(<1..215)
                     /locus_tag="ACG842_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase domain-containing protein"
                     /protein_id="HFQ0249318.1"
                     /translation="MNRIVPVLEKSLLSRDAFRVINLIICLLYFMPSTFNVTNIPMKI
                     AFAWGGIIVLYEVFIKKNFFKMRFSWLL"
     gene            complement(208..>730)
                     /locus_tag="ACG842_002883"
     CDS             complement(208..>730)
                     /locus_tag="ACG842_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="beta-1,6-N-acetylglucosaminyltransferase"
                     /protein_id="HFQ0249319.1"
                     /translation="INRNNTTAFKIFRAIINRCLRMIKLPPKIRHHKFDLYHGSQWWA
                     LSKECLTELIQMYEQNQQDYLNFKIGMFAPDEKFFHTLFFNSSFKNKNVIGGPDLPLE
                     LKNIEETTLQTSKLANIHIIDPSMNKWFDERDIDQIKESEKLFVRKVKSDYSDKLLNR
                     IDKEILKVGNIDE"
BASE COUNT          255 a          116 c           85 g          274 t
ORIGIN      
        1 agtagccaag aaaaacgcat tttaaaaaaa tttttcttta taaaaacctc atacaaaact
       61 ataattccac cccaagcaaa agctattttc atcgggatat tagttacatt aaaagtacta
      121 ggcataaaat aaagcagaca aatgattaaa tttattactc gaaacgcatc tcgacttaat
      181 aaactcttct ctaaaacagg tactattcta ttcatctata tttccaactt tcaaaatttc
      241 tttatcaatt ctatttaata gtttatcaga gtaatccgat ttaacttttc ttacaaataa
      301 tttttcactt tcttttattt gatctatatc cctttcatca aaccatttgt tcatggaagg
      361 atcaataata tgaatatttg ctaatttcga agtttgtaaa gtagtttctt caatattttt
      421 caattcaagt ggtaaatctg gtcctccaat aacattttta tttttgaacg aggaattaaa
      481 aaaaagtgta tgaaaaaatt tttcatctgg ggcaaacata cctattttaa aattcaggta
      541 gtcttgttga ttttgttcat acatttgtat taattctgtt aggcattctt tcgacaaagc
      601 ccaccattga gaaccatgat acaaatcaaa tttatggtgt ctaatttttg ggggaagttt
      661 aatcatcctc agacaacgat taattatagc tctaaaaatt ttaaatgcag ttgtgttatt
      721 cctattaatt
//
LOCUS       DAZKLM010000233          725 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3764
            SAMEA115706913-rid22582563.denovo.244, whole genome shotgun
            sequence.
ACCESSION   DAZKLM010000233 DAZKLM010000000
VERSION     DAZKLM010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706913
            Sequence Read Archive: ERR13196647
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 725)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 725)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287823.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287823.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,004
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,849
            CDSs (with protein)               :: 2,849
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 41 of 96
            Pseudo Genes (incomplete)         :: 52 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 15 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..725
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706913-rid22582563.denovo.244"
                     /isolate="VRE3764"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>725
                     /locus_tag="ACG842_002884"
     CDS             <1..>725
                     /locus_tag="ACG842_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tip lysozyme"
                     /protein_id="HFQ0249320.1"
                     /translation="GFTKQSACGILGNIQQETGGTFDPDTVQIGGPAYGLVQWDGSSY
                     PLVGPATWDGKVYVQNLFNAAGIKEPITSLDAQVRLLIWTFTNGQWMGVVQPTTVDGF
                     KACTDPRQAAYAFERNYERPAATHPERQDYAVNWYNKFKDLKPGGATGEAGLKHLESL
                     IGQRIGNGQCYGLSAEYSGYLGGCGMGAGTKYGLTHVIGNTSAASDIGIAYDWSAVGW
                     KVIQNPRYDQLVVGAIINWARGG"
BASE COUNT          226 a          112 c          179 g          208 t
ORIGIN      
        1 ggatttacga aacaatctgc atgtggaata ttggggaaca ttcaacaaga aacaggagga
       61 acttttgatc ctgatactgt tcaaataggt ggaccagcat acggattagt tcagtgggat
      121 ggttcttcat atcctcttgt tggtccagca acatgggatg gaaaagttta tgttcaaaac
      181 ttatttaatg ctgcaggtat taaagaacca ataacgagct tagatgcaca agttcgattg
      241 cttatttgga catttacaaa tggacaatgg atgggcgtag tacaacctac gacggttgat
      301 ggatttaagg cctgtactga tccaagacaa gcagcatatg cttttgaacg aaactatgaa
      361 cgtccggcag cgacacatcc tgaacgtcaa gattatgcag ttaactggta taacaaattt
      421 aaagatttaa aaccaggagg agctactgga gaagcaggac taaaacattt agagtctttg
      481 attggacaac gaattggcaa tggtcagtgt tatggcttgt ctgcagaata ttcaggatat
      541 ctgggtggtt gtggcatggg tgctggaaca aaatatggtt taactcatgt gataggaaat
      601 acttctgcag catctgatat tggtattgcc tatgattggt ctgctgttgg ttggaaagtg
      661 attcaaaacc ctagatatga tcaattagta gttggtgcaa ttattaattg ggcaagaggt
      721 ggaca
//
LOCUS       DAZKLM010000234          707 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3764
            SAMEA115706913-rid22582563.denovo.245, whole genome shotgun
            sequence.
ACCESSION   DAZKLM010000234 DAZKLM010000000
VERSION     DAZKLM010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706913
            Sequence Read Archive: ERR13196647
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 707)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 707)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287823.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287823.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,004
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,849
            CDSs (with protein)               :: 2,849
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 41 of 96
            Pseudo Genes (incomplete)         :: 52 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 15 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..707
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706913-rid22582563.denovo.245"
                     /isolate="VRE3764"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..598
                     /locus_tag="ACG842_002885"
     CDS             <1..598
                     /locus_tag="ACG842_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFQ0249321.1"
                     /translation="MKKEFPIPISWTTDVNAAAYGEYVFGSGKGLSSVVYYTIGTGVG
                     GGALQEGRFIEGFSHPEMGHMLVVPHPKDDFAGSCPFHGNCLEGMAAGPAIEKRLGKK
                     GQEVAEDDPYWEIEASYIAQCAYNTTLMFSPDVIIFGGGVMKQRHMLQNVHDAFAKLV
                     NGYVETPELSKYIVTPALEDNAGTLGCLALAREAVLHS"
BASE COUNT          199 a          139 c          180 g          189 t
ORIGIN      
        1 catgaaaaaa gaatttccga tcccgatttc ttggacaaca gatgtcaatg cggctgccta
       61 tggtgagtat gtcttcggca gtggcaaagg attgagcagt gtcgtttact atacgatcgg
      121 tacgggtgtt ggcggcggtg ccttacaaga agggcgcttt atcgaagggt ttagtcatcc
      181 agaaatgggg catatgctcg tcgtccctca tccaaaagat gactttgctg gcagctgtcc
      241 atttcatggc aactgtttag aagggatggc cgctggaccc gccatcgaaa aacgcttagg
      301 gaaaaaagga caagaggttg cagaagatga tccttattgg gaaatcgaag cctcctatat
      361 tgctcaatgt gcctacaata caaccttgat gttttctcca gatgtgatca tttttggcgg
      421 tggtgtgatg aaacaacgcc atatgctgca aaacgtccac gacgcatttg caaaattggt
      481 caatgggtat gtcgaaacac ctgagttaag caaatatatc gtgaccccag cattagaaga
      541 caacgctgga acgctcggtt gtttggcatt ggctcgagaa gcggtcttac attcataaac
      601 gaaaaaaggc tcttcgacat ttagtgttgg tagagaaaga tacaactgaa aatttagtca
      661 taaccagtta ggcagagtgc ttaactggtt tttgttttac ttggaag
//
LOCUS       DAZKLM010000235          682 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3764
            SAMEA115706913-rid22582563.denovo.246, whole genome shotgun
            sequence.
ACCESSION   DAZKLM010000235 DAZKLM010000000
VERSION     DAZKLM010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706913
            Sequence Read Archive: ERR13196647
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 682)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 682)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287823.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287823.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,004
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,849
            CDSs (with protein)               :: 2,849
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 41 of 96
            Pseudo Genes (incomplete)         :: 52 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 15 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..682
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706913-rid22582563.denovo.246"
                     /isolate="VRE3764"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(284..583)
                     /locus_tag="ACG842_002886"
     CDS             complement(284..583)
                     /locus_tag="ACG842_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HFQ0249322.1"
                     /translation="MSKSEEIENMVLKLYDLVLNPEIKEKERILLIDAKTGLEKGQYY
                     PKVINNLERSLRPLAIRGELSKPVSPFYMEISTIGKFEKELGRGMASAPITFGHL"
BASE COUNT          225 a          118 c           73 g          266 t
ORIGIN      
        1 attttactta ttgctttcat tttttcaatc tatatttcca atttttaact tacaaaaatg
       61 caacattaat aaataacgat agtttcataa aatacacttt taattaacat tattagttta
      121 tactagctgt ttttgtgtta attaaaggat aaaatacaat ataaagaaac atctgtttct
      181 ttatttagaa taaaaaaata cagactctgt tcaaataaat gaacctagcc tgcattatga
      241 gattgactat actttttaat tcattgagat gaataactcg tttttataaa tgcccaaaag
      301 taatcggtgc cgaagccatt cctcgaccta attctttttc aaattttcct attgttgata
      361 tctccatata aaacggtgat actggtttag acaattctcc cctgattgct aacggacgta
      421 aactacgttc caaattattt atgactttag gataatactg acctttttct aagcctgtct
      481 ttgcgtctat caaaagtata cgttcctttt ctttgatctc tggatttaaa acgagatcat
      541 ataattttag taccatgttt tctatttctt cacttttaga catcacgatt tcctcccaaa
      601 aatatatttc ttcccactac attctataaa caaagtatac gctacatcta gattttttta
      661 aatcattact ggattgttaa aa
//
LOCUS       DAZKLM010000236          656 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3764
            SAMEA115706913-rid22582563.denovo.247, whole genome shotgun
            sequence.
ACCESSION   DAZKLM010000236 DAZKLM010000000
VERSION     DAZKLM010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706913
            Sequence Read Archive: ERR13196647
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 656)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 656)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287823.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287823.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,004
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,849
            CDSs (with protein)               :: 2,849
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 41 of 96
            Pseudo Genes (incomplete)         :: 52 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 15 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..656
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706913-rid22582563.denovo.247"
                     /isolate="VRE3764"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(138..491)
                     /gene="tnpB"
                     /locus_tag="ACG842_002887"
     CDS             complement(138..491)
                     /gene="tnpB"
                     /locus_tag="ACG842_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFQ0249323.1"
                     /translation="MLYDLLQTKHIYIVCGKTDLRKGIDGLASLIQQEYQLELYEDAV
                     FLFCGNRQDRFKLLYWDGDGFLLCYKRIENGKLKWPRTKDEVRTLTNQQVRWLLEGLS
                     IDQPRAILPGKKGVF"
BASE COUNT          198 a          143 c          111 g          204 t
ORIGIN      
        1 ttgaagcaac tgaagcaacg tatctgtagt ttccaaaatt tttcaccacc ttgaatcgaa
       61 ctgattttgt tacagaatac cacaagaaac gcacaagaaa taggggttca acacattttt
      121 tgtgttaacc ccccagctta aaaaactcct ttttttcctg gcaaaatagc tcgaggttga
      181 tcgatactta atccttctaa caaccatcga acctgttggt tcgttaaggt tcgaacttcg
      241 tctttcgtcc gaggccattt taacttgccg ttttctatcc gcttataaca gagaagaaaa
      301 ccatcaccgt cccaatagag aagtttgaaa cggtcttgac ggttcccgca aaataaaaaa
      361 acggcatctt cgtaaagctc gagttgatac tcttgttgaa taagactggc aagaccatca
      421 attccttttc gcaaatctgt ttttccgcac acaatataaa tatgtttggt ttgaagtaaa
      481 tcataaagca ttggacaaca gttctttcaa aatagcttga agaatataat tgttaatacc
      541 gttatagatc aaaacttcag aaccattctc tcttttgatg cgcatcgcta acttggcagc
      601 cgcgggttta cctttttctg gtgcactctt ttcgtttcct aattggacag gaacaa
//
LOCUS       DAZKLM010000237          643 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3764
            SAMEA115706913-rid22582563.denovo.248, whole genome shotgun
            sequence.
ACCESSION   DAZKLM010000237 DAZKLM010000000
VERSION     DAZKLM010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706913
            Sequence Read Archive: ERR13196647
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 643)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 643)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287823.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287823.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,004
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,849
            CDSs (with protein)               :: 2,849
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 41 of 96
            Pseudo Genes (incomplete)         :: 52 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 15 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..643
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706913-rid22582563.denovo.248"
                     /isolate="VRE3764"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            134..331
                     /locus_tag="ACG842_002888"
     CDS             134..331
                     /locus_tag="ACG842_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II bacteriocin"
                     /protein_id="HFQ0249324.1"
                     /translation="MKHLKILSIKETQLIYGGTTHSGKYYGNGVYCTKNKCTVDWAKA
                     TTCIAGMSIGGFLGGAIPGKC"
     gene            333..>643
                     /locus_tag="ACG842_002889"
     CDS             333..>643
                     /locus_tag="ACG842_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HFQ0249325.1"
                     /translation="MKKNAKQIVHELYNDISISKDPKYSDILEVLQKVYLKLEKQKYE
                     LDPGPLINRLVNYLYFTAYTNKIRFTEYQEELIRNLSEIGRTAGINGLYRADYGDKSQ
                     F"
BASE COUNT          248 a           61 c          116 g          218 t
ORIGIN      
        1 gtttatatac ttaaaagtga ttaaattctt attcaggaat tattttgcta cgtttcaaga
       61 aggaattgtt ttttgaaatt taatgaatgt tagaataata gtctaaaaac attaatttag
      121 gggtgattag attatgaaac atttaaaaat tttgtctatt aaagagacac aacttatcta
      181 tgggggtacc actcatagtg gaaaatatta tggaaatgga gtgtattgca ctaaaaataa
      241 atgtacggtc gattgggcca aggcaactac ttgtattgca ggaatgtcta taggtggttt
      301 tttaggtgga gcaattccag ggaagtgcta aaatgaaaaa aaatgctaag caaattgttc
      361 atgaattata taatgatata tctataagta aagatcctaa atattctgat attcttgagg
      421 ttttacaaaa ggtatattta aaattagaaa aacaaaaata tgaattagat cccggtcctt
      481 taataaatag attggtgaat tatctatatt ttactgctta tactaataaa ataagattca
      541 ctgaatatca agaggaatta ataagaaatt tgagtgaaat tggaagaact gctggaataa
      601 atggtttata tcgagcagat tatggagata aatctcaatt tta
//
LOCUS       DAZKLM010000238          600 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3764
            SAMEA115706913-rid22582563.denovo.249, whole genome shotgun
            sequence.
ACCESSION   DAZKLM010000238 DAZKLM010000000
VERSION     DAZKLM010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706913
            Sequence Read Archive: ERR13196647
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 600)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 600)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287823.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287823.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,004
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,849
            CDSs (with protein)               :: 2,849
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 41 of 96
            Pseudo Genes (incomplete)         :: 52 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 15 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..600
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706913-rid22582563.denovo.249"
                     /isolate="VRE3764"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..568
                     /locus_tag="ACG842_002890"
     CDS             <1..568
                     /locus_tag="ACG842_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0249326.1"
                     /translation="YDVKNIKKIPKIDENSKLMKNIQSIFGIEEVFVGDKCIFFDQPL
                     LSDGYGIDETDIFAKVNSIFSGKKETLIKLHPRARTNRYGEKNILNSSLPFEVLLLEN
                     DFSSTLLISPISTISFSPSMMFEKTINSIMLAKLILNEYTALSEDKKETLTNIVLFCE
                     HYNRLSEEKILLPENWAELRKLVEYGII"
BASE COUNT          231 a           81 c           89 g          199 t
ORIGIN      
        1 ttatgatgta aaaaatataa aaaaaattcc caaaatagat gaaaacagta aattgatgaa
       61 aaacatacaa tcaatctttg ggatagaaga agtttttgtc ggagataaat gtatcttttt
      121 tgatcaacct ttattatctg atggttatgg catagatgaa acagatattt tcgctaaagt
      181 aaatagtata ttttctggaa aaaaagaaac tttaattaag cttcatccta gagcaaggac
      241 aaatcgttat ggtgaaaaaa acatattgaa ttccagttta ccgtttgaag tcttgctact
      301 tgaaaatgac ttctcttcaa cacttttgat tagtcctata tctacaattt ctttttcacc
      361 atctatgatg tttgaaaaga cgatcaactc tattatgttg gctaaattaa ttttaaatga
      421 atatacagca ttatctgaag ataaaaaaga aacattgaca aatattgttc ttttctgtga
      481 acattataac cgtctatcag aagaaaaaat tttactccca gaaaactggg cagaattaag
      541 aaagttggta gagtatggga ttatttaaca cactttccaa gtcaaaatta gttaagtcca
//
LOCUS       DAZKLM010000239          582 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3764
            SAMEA115706913-rid22582563.denovo.250, whole genome shotgun
            sequence.
ACCESSION   DAZKLM010000239 DAZKLM010000000
VERSION     DAZKLM010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706913
            Sequence Read Archive: ERR13196647
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 582)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 582)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287823.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287823.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,004
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,849
            CDSs (with protein)               :: 2,849
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 41 of 96
            Pseudo Genes (incomplete)         :: 52 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 15 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..582
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706913-rid22582563.denovo.250"
                     /isolate="VRE3764"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(37..>582)
                     /locus_tag="ACG842_002891"
     CDS             complement(37..>582)
                     /locus_tag="ACG842_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFQ0249327.1"
                     /translation="IHRRFYTSICHQKITTDTTEFKYHEVDAKGIIRQKKLYLDPFMD
                     MFNSEILSYRISEKPNALAVMEGLEEAIQTTNDCPYRRTFHSDQGWAYQMKAYRNKLK
                     EYKIFQSMSRKGNCLDNSLMENFFGLLKQEIFHGKVYNCFEELKSAIDSYIYYYNNER
                     IKQKLNWQSPVQFRKTTVTVV"
BASE COUNT          166 a          110 c          100 g          206 t
ORIGIN      
        1 aatccactcc ttttttcatg ctctaaaatg atatttttac acaactgtta cggttgtttt
       61 acgaaattgg acgggacttt gccagtttaa tttctgtttt atccgttcat tattgtagta
      121 atagatatag ctatcgatgg ctgattttaa ttcctcgaaa cagttataaa ctttcccgtg
      181 aaaaatctct tgttttaata agccaaaaaa attctccata agtgaattat ctaagcagtt
      241 ccctttccga gacatgcttt gaaagatttt gtactccttc aatttgtttc tataagcttt
      301 catttgatac gcccaacctt gatctgagtg aaacgtacga cgatagggac aatcgttagt
      361 ggtctggata gcttcctcca aaccttccat taccgctaat gcattgggct tttcagagat
      421 acgataggat aaaatctcac tattgaacat atccataaaa ggatctagat acaatttctt
      481 ttgacggata atgcctttgg catccacttc atggtattta aattcagttg tatccgtcgt
      541 gattttctga tggcagatac tggtgtagaa acgacgatgg at
//
LOCUS       DAZKLM010000240          560 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3764
            SAMEA115706913-rid22582563.denovo.251, whole genome shotgun
            sequence.
ACCESSION   DAZKLM010000240 DAZKLM010000000
VERSION     DAZKLM010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706913
            Sequence Read Archive: ERR13196647
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 560)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 560)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287823.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287823.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,004
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,849
            CDSs (with protein)               :: 2,849
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 41 of 96
            Pseudo Genes (incomplete)         :: 52 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 15 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..560
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706913-rid22582563.denovo.251"
                     /isolate="VRE3764"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            136..534
                     /gene="spxA"
                     /locus_tag="ACG842_002892"
     CDS             136..534
                     /gene="spxA"
                     /locus_tag="ACG842_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SpxA"
                     /protein_id="HFQ0249328.1"
                     /translation="MLTLYTSPSCTSCRKARAWLQEHQIPFVERNIFSEPLNSSELKA
                     ILQMTEDGTEEIISTRSKVFQKLNMDLDDLPLQELLELVQNNPGLLRRPIMIDDKRLQ
                     VGFNEDEIRRFLPRDVRQLELRQAQLMAGL"
BASE COUNT          197 a           87 c          110 g          166 t
ORIGIN      
        1 ttcatttttt taaaaaaatt atgaaaaaaa ggtagctatt taaaagttga ttatgtataa
       61 tagaaacaga tgggagatta ttatgatctt ctgttacaaa aaagtgattc ttatcacgaa
      121 aaggagtggt gcacgttgtt aacattatat acttccccaa gttgtacttc ctgcagaaag
      181 gctcgtgcgt ggttgcaaga acatcagatt ccatttgttg aacgtaatat tttttcagaa
      241 ccattgaaca gttctgaatt aaaagcgatt ttacaaatga cagaagacgg aacagaagaa
      301 atcatctcga cacgttccaa agtattccaa aaactgaaca tggatttaga tgatctgccg
      361 ttacaagaat tattggaact tgtgcaaaac aatcctggat tgcttcgccg tccaatcatg
      421 attgatgata aacgtctgca agttggtttt aatgaagatg aaattcgtcg gttcctaccg
      481 cgcgatgtac gacaattaga attacgtcaa gcacaattga tggctggatt atagaaggat
      541 aatacgaaac ttggaaaaga
//
LOCUS       DAZKLM010000241          528 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3764
            SAMEA115706913-rid22582563.denovo.252, whole genome shotgun
            sequence.
ACCESSION   DAZKLM010000241 DAZKLM010000000
VERSION     DAZKLM010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706913
            Sequence Read Archive: ERR13196647
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 528)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 528)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287823.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287823.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,004
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,849
            CDSs (with protein)               :: 2,849
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 41 of 96
            Pseudo Genes (incomplete)         :: 52 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 15 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..528
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706913-rid22582563.denovo.252"
                     /isolate="VRE3764"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            76..198
                     /locus_tag="ACG842_002893"
     CDS             76..198
                     /locus_tag="ACG842_002893"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0249329.1"
                     /translation="MHKPFPDPIQKAIDELGIKNKKRFILVIHYMTCKVRKLVG"
     gene            171..302
                     /locus_tag="ACG842_002894"
     CDS             171..302
                     /locus_tag="ACG842_002894"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0249330.1"
                     /translation="MQSAKACGIAFGLAKWGAKNIENFQSVDIIIDSPQEILDLLEK"
BASE COUNT          198 a           80 c           93 g          157 t
ORIGIN      
        1 ctctaggaaa aaattaacta gcaccacggg ttaaatctat attttgatat acaaattaca
       61 gcttcagata ctaagttgca taaacctttt ccggacccca tacaaaaggc aattgatgaa
      121 ttgggcataa aaaataagaa acgatttata ttggtgattc attatatgac atgcaaagtg
      181 cgaaagcttg tgggatagca tttggtttag ctaaatgggg agccaaaaat atagaaaatt
      241 ttcaaagtgt agatataatt attgattctc ctcaagaaat acttgatttg ttagaaaaat
      301 agacaaagag cgtgagataa ggatagggtc tcacgctctt tgtttttcgc catttaaaga
      361 ttgaaatttt tgccatcgcc gattgtaaaa ttaagctaga caactaaaaa agtcatttgt
      421 gctacactca aaatagttcc gaccaaagaa ttataaggag tgagtacaaa tgacctatac
      481 ccatcttaca acggatgaac ttgtaataat agagtcttat ttcaaaat
//
LOCUS       DAZKLM010000242          518 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3764
            SAMEA115706913-rid22582563.denovo.253, whole genome shotgun
            sequence.
ACCESSION   DAZKLM010000242 DAZKLM010000000
VERSION     DAZKLM010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706913
            Sequence Read Archive: ERR13196647
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 518)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 518)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287823.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287823.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,004
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,849
            CDSs (with protein)               :: 2,849
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 41 of 96
            Pseudo Genes (incomplete)         :: 52 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 15 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..518
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706913-rid22582563.denovo.253"
                     /isolate="VRE3764"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(36..338)
                     /locus_tag="ACG842_002895"
     CDS             complement(36..338)
                     /locus_tag="ACG842_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5960 family protein"
                     /protein_id="HFQ0249331.1"
                     /translation="MLMEGKTQLWFKFDPSNRFVKDFYKVWDSEVFFLAIEDSLLINL
                     YYSNKNYFKIPATKTRMKKDVYFLFDIVTDVPDARSDHRRYDYLKYTFVDPERYKD"
BASE COUNT          177 a           92 c           71 g          178 t
ORIGIN      
        1 atccgttccg ggctaccttt tttaggtagc ctactttaat ctttatacct ttctggatca
       61 acgaaagtat acttcaaata gtcataacgc cgatgatcgc tccgtgcgtc tggcacatca
      121 gtcacgatat caaacaaaaa atacacatct ttcttcattc tggtttttgt tgccggaatt
      181 ttaaagtagt tcttattaga gtaatagaga ttgattaata agctatcttc gattgctaaa
      241 aagaaaactt ctgaatccca caccttataa aaatctttga caaatctatt cgaaggatca
      301 aatttaaacc ataattgtgt ttttccttcc atcaacataa tgttcacctc aaaaagagta
      361 tacgaactaa tgttcttttt gtaaagtagg aatatcattg ataatatgga tggaacatat
      421 atattaaaaa gtgttctttt tgaatgctat tttgaatgcc attttcattc atttaacgaa
      481 aatctgcgaa aataaaaaat gctcaaaccc ttataaaa
//
LOCUS       DAZKLM010000243          510 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3764
            SAMEA115706913-rid22582563.denovo.254, whole genome shotgun
            sequence.
ACCESSION   DAZKLM010000243 DAZKLM010000000
VERSION     DAZKLM010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706913
            Sequence Read Archive: ERR13196647
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 510)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 510)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287823.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287823.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,004
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,849
            CDSs (with protein)               :: 2,849
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 41 of 96
            Pseudo Genes (incomplete)         :: 52 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 15 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..510
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706913-rid22582563.denovo.254"
                     /isolate="VRE3764"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..343
                     /locus_tag="ACG842_002896"
     CDS             <1..343
                     /locus_tag="ACG842_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001226076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0249332.1"
                     /translation="DNIRDKKASLKSLKDTWLDLSEDNPYSQFLITVMAGVNQLERDL
                     IRMRQREGIELAKKEGKFKGRLKKYHKNHAGMNYAVKLYKEGNMTVNQICEITNVSRA
                     SLYRKLSEVNN"
BASE COUNT          205 a           69 c           93 g          143 t
ORIGIN      
        1 cgataacata cgagataaaa aggcaagttt aaaatcacta aaagatacat ggcttgattt
       61 atcagaagat aatccataca gccaattctt aattactgta atggctggtg ttaaccaatt
      121 agagcgagat cttattcgga tgagacaacg tgaagggatt gaattggcta agaaagaagg
      181 aaagtttaaa ggtcgattaa agaagtatca taaaaatcac gcaggaatga attatgcggt
      241 aaagctatat aaagaaggaa atatgactgt aaatcaaatt tgtgaaatta ctaatgtatc
      301 tagggcttca ttatacagga aattatcaga agtgaataat tagccattct gtattccgct
      361 aatgggcaat atttttaaag aagaaaagga aactataaaa tattaacagc ctcctagcga
      421 tgccgaaaag ccctttgata aaaaaagaat catcatctta agaaattctt agtcatttat
      481 tatgtaaatg cttataaatt cggccctata
//
LOCUS       DAZKLM010000244          506 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3764
            SAMEA115706913-rid22582563.denovo.256, whole genome shotgun
            sequence.
ACCESSION   DAZKLM010000244 DAZKLM010000000
VERSION     DAZKLM010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706913
            Sequence Read Archive: ERR13196647
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 506)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 506)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287823.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287823.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,004
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,849
            CDSs (with protein)               :: 2,849
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 41 of 96
            Pseudo Genes (incomplete)         :: 52 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 15 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..506
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706913-rid22582563.denovo.256"
                     /isolate="VRE3764"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>506
                     /locus_tag="ACG842_002897"
     CDS             <1..>506
                     /locus_tag="ACG842_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IS3-like element IS1485 family transposase"
                     /protein_id="HFQ0249333.1"
                     /translation="DLTYVRVGKKWNYVCFILDLFNREILGYSCGEHKDAVLVKKAFS
                     RIKQPLTEVEIFHTDRGKEFDNQAIDELLTTFDINRSLSHKGCPFDNAVAESTYKSLK
                     VEFVYQYTFETLQQLDLELFDYVNWWNHLRLHGTLGYETPVGYRNQRLAQRILDNELG
                     CANASEAV"
BASE COUNT          153 a           89 c          110 g          154 t
ORIGIN      
        1 agacttgact tatgtacgag tcgggaaaaa atggaattat gtctgtttca ttttggatct
       61 gttcaatcga gaaattctcg gctattcttg tggagaacat aaagatgccg ttctagtaaa
      121 aaaagcattt agccgtatca aacaacctct gacagaggtt gagatttttc atactgatcg
      181 tggaaaagag tttgataacc aagctattga tgaattatta acaacttttg acatcaatcg
      241 atcattgagt cataaaggct gtccttttga taatgccgta gctgaatcaa cttataagtc
      301 gttgaaagta gaatttgtct atcaatacac atttgaaacc ttacaacaat tggatttgga
      361 gttatttgac tatgtcaatt ggtggaacca ccttcggttg cacggtacac ttggctacga
      421 gacaccggtt ggttaccgta accagagatt ggcgcagcga atccttgata atgagctcgg
      481 atgtgctaac gctagcgagg cagtct
//
LOCUS       DAZKLM010000245          501 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3764
            SAMEA115706913-rid22582563.denovo.257, whole genome shotgun
            sequence.
ACCESSION   DAZKLM010000245 DAZKLM010000000
VERSION     DAZKLM010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706913
            Sequence Read Archive: ERR13196647
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 501)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 501)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287823.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287823.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,004
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,849
            CDSs (with protein)               :: 2,849
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 41 of 96
            Pseudo Genes (incomplete)         :: 52 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 15 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..501
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706913-rid22582563.denovo.257"
                     /isolate="VRE3764"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(86..322)
                     /locus_tag="ACG842_002898"
                     /pseudo
     CDS             complement(86..322)
                     /locus_tag="ACG842_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          169 a           84 c           87 g          161 t
ORIGIN      
        1 tacaaaaaag atgttggtga agttcaccaa catcaaaaag tatagagccg atagaaggac
       61 tgaccttctg tctcagattt ttttgttatt tcacttgaat caattgattg atcaaaaaaa
      121 tcctgatttt catgttctca aatgatttaa atccataaga taatcgtttc agtgtcttga
      181 tatggggaat cttggcctct attttgccat tagaatacgg gtaaatcata gcattgtaaa
      241 taccttcttc atacgttaaa aggttttgaa gtttcgtgcg aagttgtgca tcaagtgttt
      301 caggaagatt ttttaataac gccgtttgta taattaagcg agacaaataa aaaagccatt
      361 tatgttacac tccaattgta atacccgcta aagcatacaa ggagagtgaa cataaatgac
      421 ttatacccat cttacaccaa acgagcttgt aatgatagaa gcatattttc atcaagaaac
      481 tccggttgct atcgttgcga a
//
LOCUS       DAZKLM010000246          469 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3764
            SAMEA115706913-rid22582563.denovo.258, whole genome shotgun
            sequence.
ACCESSION   DAZKLM010000246 DAZKLM010000000
VERSION     DAZKLM010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706913
            Sequence Read Archive: ERR13196647
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 469)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 469)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287823.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287823.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,004
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,849
            CDSs (with protein)               :: 2,849
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 41 of 96
            Pseudo Genes (incomplete)         :: 52 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 15 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..469
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706913-rid22582563.denovo.258"
                     /isolate="VRE3764"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
BASE COUNT          152 a           90 c           56 g          171 t
ORIGIN      
        1 ggcgaaagca caaaaaataa aaaaattatt acgttattaa aaaaacagat gaaagatcag
       61 gttttattag atctcattct ttcatcactt gaagggattt aatgaactac tttttcctct
      121 ttgtcgtttt tatatattcg tattccttca aacagtagga tagcctcttt caatgcttga
      181 tatttgaact gtccgttact taaaaattta gatgtgccgc cctcttccta gaacatattc
      241 ttttttactt tttcattaat tgacagacca atcgaacgaa actgtaaatt tcgataggat
      301 aatgattggt caaactcatt cattttcaag acatctcgat tcatattcaa gataggaatc
      361 tttttatcaa catcacttta ttcgtatttt cctctaccat aaaagcttca cgctccacaa
      421 aattgtttta tacactacta ttcagcccat tcaacgacca atgaatttt
//
LOCUS       DAZKLM010000247          460 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3764
            SAMEA115706913-rid22582563.denovo.259, whole genome shotgun
            sequence.
ACCESSION   DAZKLM010000247 DAZKLM010000000
VERSION     DAZKLM010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706913
            Sequence Read Archive: ERR13196647
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 460)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 460)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287823.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287823.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,004
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,849
            CDSs (with protein)               :: 2,849
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 41 of 96
            Pseudo Genes (incomplete)         :: 52 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 15 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..460
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706913-rid22582563.denovo.259"
                     /isolate="VRE3764"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(20..91)
                     /locus_tag="ACG842_002899"
     tRNA            complement(20..91)
                     /locus_tag="ACG842_002899"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(56..58),aa:Glu,seq:ttc)
     gene            complement(113..200)
                     /locus_tag="ACG842_002900"
     tRNA            complement(113..200)
                     /locus_tag="ACG842_002900"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(164..166),aa:Ser,seq:gct)
     gene            complement(225..298)
                     /locus_tag="ACG842_002901"
     tRNA            complement(225..298)
                     /locus_tag="ACG842_002901"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(262..264),aa:Ile,seq:gat)
     gene            complement(312..382)
                     /locus_tag="ACG842_002902"
     tRNA            complement(312..382)
                     /locus_tag="ACG842_002902"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(348..350),aa:Gly,seq:tcc)
     gene            complement(384..459)
                     /locus_tag="ACG842_002903"
     tRNA            complement(384..459)
                     /locus_tag="ACG842_002903"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(424..426),aa:Asp,seq:gtc)
BASE COUNT          132 a          128 c          108 g           92 t
ORIGIN      
        1 taaaactata ttaaattaat gacccgtacg ggattcgaac ccgtgttacc gccgtgaaag
       61 ggcggtgtct taaccgcttg accaacgggc caggtaaaac aaaatattga aacggagaag
      121 gagggatttg aaccctcgcg ccggtttccc gacctacacc cttagcaggg gcgcctcttc
      181 agccacttga gtacttcccc aaaaccaata agattttgta ctaatgggcc taaatggact
      241 cgaaccatcg acctcacgct tatcaggcgt gcgctctaac cagctgagct ataggcccat
      301 aaaaaactaa aagcgggtga cgagaatcga actcgcgaca acagcttgga aggctgtggt
      361 tttaccacta aactacaccc gcatggcggt tccgacggga atcgaacccg cgatctcctg
      421 cgtgacaggc aggcatgtta acccctacac cacggaacca
//
LOCUS       DAZKLM010000248          437 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3764
            SAMEA115706913-rid22582563.denovo.260, whole genome shotgun
            sequence.
ACCESSION   DAZKLM010000248 DAZKLM010000000
VERSION     DAZKLM010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706913
            Sequence Read Archive: ERR13196647
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 437)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 437)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287823.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287823.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,004
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,849
            CDSs (with protein)               :: 2,849
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 41 of 96
            Pseudo Genes (incomplete)         :: 52 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 15 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..437
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706913-rid22582563.denovo.260"
                     /isolate="VRE3764"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            1..437
                     /locus_tag="ACG842_002904"
                     /pseudo
     CDS             1..437
                     /locus_tag="ACG842_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612146.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          179 a           77 c           80 g          101 t
ORIGIN      
        1 atcttacgcc tgaacaaaaa gaattgttag aactacgtaa aagaaaccag caattagaaa
       61 tggaaaatga tattttaaag caagcagcgc tgatattcgg acgaagagac aagtaatcga
      121 tgcgaataag catctttacc ctatatcagc gatgtgcaga atattaggtc tatcacgtca
      181 gtcctattat tatcaatcaa aaccaaagaa agacgaatca gaacttgaag aagtagtcgc
      241 tgaagaattt atccgcagcc gaaaggccta cggctcaaga aaaataaaaa aagccttatc
      301 aaaacgaggc attcagatca gccgacgaaa aattagtaga atcatgaaaa atagaggatt
      361 aaaatcgagc tatactgttg cttattttaa agtacatcat tctacttgca atgaagccaa
      421 aacgacaaac gtattga
//
LOCUS       DAZKLM010000249          421 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3764
            SAMEA115706913-rid22582563.denovo.261, whole genome shotgun
            sequence.
ACCESSION   DAZKLM010000249 DAZKLM010000000
VERSION     DAZKLM010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706913
            Sequence Read Archive: ERR13196647
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 421)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 421)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287823.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287823.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,004
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,849
            CDSs (with protein)               :: 2,849
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 41 of 96
            Pseudo Genes (incomplete)         :: 52 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 15 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..421
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706913-rid22582563.denovo.261"
                     /isolate="VRE3764"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(170..355)
                     /locus_tag="ACG842_002905"
                     /pseudo
     CDS             complement(170..355)
                     /locus_tag="ACG842_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit S"
BASE COUNT          120 a           85 c           62 g          154 t
ORIGIN      
        1 tgcaacagaa ccggtaaatg aatgaaaaag atggaagaat cattattcaa tagctttctt
       61 aaatactccg ttataaaatg tgaacaagta aaaagacatc tatccctatt ttctacactt
      121 ccactttact actatctttg aatcgtacaa ttttcttaac tcttctcgtt tacctcaatt
      181 cattttcaag tggaatcaac tcatcatcta acattttacg ccatgctcgc ttcatttctt
      241 gtgggtattt aaatggtttc gcttctggat gtttggcttt atatccttcg taatccttac
      301 tgttgataat tgctttcata ttaggaattg gatcttcacc tggcatatat tgcagaggtt
      361 ctgttgcgaa gtttgaaaat caaacgcgcc ctctctgaac tccaaatatc ttaggctgtt
      421 a
//
LOCUS       DAZKLM010000250          411 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3764
            SAMEA115706913-rid22582563.denovo.262, whole genome shotgun
            sequence.
ACCESSION   DAZKLM010000250 DAZKLM010000000
VERSION     DAZKLM010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706913
            Sequence Read Archive: ERR13196647
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 411)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 411)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287823.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287823.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,004
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,849
            CDSs (with protein)               :: 2,849
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 41 of 96
            Pseudo Genes (incomplete)         :: 52 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 15 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..411
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706913-rid22582563.denovo.262"
                     /isolate="VRE3764"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>411
                     /locus_tag="ACG842_002906"
     rRNA            <1..>411
                     /locus_tag="ACG842_002906"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          108 a           91 c          131 g           81 t
ORIGIN      
        1 gaaagccccc ggctcaaccg gggagggtca ttggaaactg ggagacttga gtgcagaaga
       61 ggagagtgga attccatgtg tagcggtgaa atgcgtagat atatggagga acaccagtgg
      121 cgaaggcggc tctctggtct gtaactgacg ctgaggctcg aaagcgtggg gagcaaacag
      181 gattagatac cctggtagtc cacgccgtaa acgatgagtg ctaagtgttg gagggtttcc
      241 gcccttcagt gctgcagcta acgcattaag cactccgcct ggggagtacg accgcaaggt
      301 tgaaactcaa aggaattgac gggggcccgc acaagcggtg gagcatgtgg tttaattcga
      361 agcaacgcga agaaccttac caggtcttga catcctttga ccactctaga g
//
LOCUS       DAZKLM010000251          408 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3764
            SAMEA115706913-rid22582563.denovo.263, whole genome shotgun
            sequence.
ACCESSION   DAZKLM010000251 DAZKLM010000000
VERSION     DAZKLM010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706913
            Sequence Read Archive: ERR13196647
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 408)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 408)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287823.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287823.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,004
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,849
            CDSs (with protein)               :: 2,849
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 41 of 96
            Pseudo Genes (incomplete)         :: 52 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 15 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..408
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706913-rid22582563.denovo.263"
                     /isolate="VRE3764"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            166..>408
                     /locus_tag="ACG842_002907"
     CDS             166..>408
                     /locus_tag="ACG842_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFQ0249334.1"
                     /translation="MLYGAIEAGGTKFVCAIGDEEMTIKERVSFPTTTPEETMPLVID
                     FFKQYQADLAGIGIGSFGPIDIHRDSATYGYITSTPK"
BASE COUNT          150 a           63 c           76 g          119 t
ORIGIN      
        1 tatcaattgt atgaaaaatt attagaattt taaaaaatga taaaaattca aaaagaaaaa
       61 atagtagtta tttacttgtc atgttactaa ataaaaaaat aatttaaata tgatatatag
      121 tcatttattt agtgatttca ttctaaaata aagggagaaa tgtctatgct atacggtgca
      181 attgaagcag gcggcacaaa atttgtgtgt gccattggcg atgaagaaat gacgatcaag
      241 gaaagagtga gctttccgac aacgacacct gaggaaacaa tgcctttagt cattgacttc
      301 ttcaaacaat accaagcaga tttagcaggg attgggattg gctcatttgg gcctatcgat
      361 atccatcgcg attccgccac ttatgggtac attacctcga ccccgaaa
//
LOCUS       DAZKLM010000252          404 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3764
            SAMEA115706913-rid22582563.denovo.264, whole genome shotgun
            sequence.
ACCESSION   DAZKLM010000252 DAZKLM010000000
VERSION     DAZKLM010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706913
            Sequence Read Archive: ERR13196647
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 404)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 404)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287823.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287823.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,004
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,849
            CDSs (with protein)               :: 2,849
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 41 of 96
            Pseudo Genes (incomplete)         :: 52 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 15 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..404
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706913-rid22582563.denovo.264"
                     /isolate="VRE3764"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>404
                     /locus_tag="ACG842_002908"
     CDS             <1..>404
                     /locus_tag="ACG842_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rib/alpha-like domain-containing protein"
                     /protein_id="HFQ0249335.1"
                     /translation="PEDKLPVLKAKDGYTDAKWPEEATQPITADDTEFVSSATKLDDK
                     SDADKYNPEGQKVTTELNKEPDAFEGIKNKEDLPKDTKYTWKEKVDVSAAGNKKGTVV
                     VTYPDGSSDEVEVDVTVTDNRSDADKYEPTVEG"
BASE COUNT          166 a           60 c           92 g           86 t
ORIGIN      
        1 ccagaagata aacttccagt gttgaaagca aaagatggtt atacagatgc gaaatggcca
       61 gaggaagcaa ctcaaccaat tacagcagat gatacagaat ttgtatcaag tgcaacaaaa
      121 ctggatgata aatctgacgc tgacaaatat aatcctgaag gtcaaaaagt gactacagaa
      181 ttgaataagg aaccagacgc atttgaggga attaagaaca aagaagattt accaaaagat
      241 actaagtata cttggaaaga aaaagtagat gttagcgcag ctggaaataa aaaagggact
      301 gttgtagtga catatccaga tggatcatct gatgaagttg aagtagatgt tacagtgaca
      361 gacaatcgtt ctgacgccga taaatatgag ccaacagtag aagg
//
LOCUS       DAZKLM010000253          386 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3764
            SAMEA115706913-rid22582563.denovo.265, whole genome shotgun
            sequence.
ACCESSION   DAZKLM010000253 DAZKLM010000000
VERSION     DAZKLM010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706913
            Sequence Read Archive: ERR13196647
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 386)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 386)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287823.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287823.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,004
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,849
            CDSs (with protein)               :: 2,849
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 41 of 96
            Pseudo Genes (incomplete)         :: 52 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 15 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..386
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706913-rid22582563.denovo.265"
                     /isolate="VRE3764"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>386
                     /locus_tag="ACG842_002909"
     CDS             <1..>386
                     /locus_tag="ACG842_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFQ0249336.1"
                     /translation="CDDNGFILSTIITPRNIHDSQVAFQLIKQSKRLFPEINCVVADA
                     EYKTPKFVHFLTHLNLRPCLPYSRPKGKKGLLSKNEFLYDEYFDCYICPQDQMLAFST
                     VTRERYREYKSNPKECVNCPLLNQCT"
BASE COUNT          120 a           80 c           67 g          119 t
ORIGIN      
        1 atgcgacgac aatggattta ttcttagcac aatcatcact cctagaaata ttcatgacag
       61 tcaagtcgcc tttcagctca tcaaacaatc gaagagactt tttcctgaaa tcaattgtgt
      121 tgtcgctgat gctgagtata agacacctaa gtttgttcac tttttgactc atttaaacct
      181 tcgaccttgt ttgccttatt caaggcccaa agggaaaaag gggctattat caaaaaatga
      241 gtttctctat gacgagtatt ttgattgtta tatttgtccg caagatcaaa tgctcgcttt
      301 ttcaacggtt actcgagaaa ggtaccgaga atacaaatcc aatccaaaag aatgtgtgaa
      361 ctgtcctctg ctaaaccaat gtacca
//
LOCUS       DAZKLM010000254          386 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3764
            SAMEA115706913-rid22582563.denovo.266, whole genome shotgun
            sequence.
ACCESSION   DAZKLM010000254 DAZKLM010000000
VERSION     DAZKLM010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706913
            Sequence Read Archive: ERR13196647
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 386)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 386)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287823.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287823.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,004
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,849
            CDSs (with protein)               :: 2,849
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 41 of 96
            Pseudo Genes (incomplete)         :: 52 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 15 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..386
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706913-rid22582563.denovo.266"
                     /isolate="VRE3764"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>386
                     /locus_tag="ACG842_002910"
     CDS             <1..>386
                     /locus_tag="ACG842_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFQ0249337.1"
                     /translation="CDDNGFILSTIITPRNIHDSQVAFQLVKQSKRLFPEINCVVADA
                     GYKTPKFVHFLTHLNLRPCLPYSRPKGKKELLSKNEFLYDEYFDCYICPQDQMLAFST
                     VTREGYREYKSNPKECVNCPLLNQCT"
BASE COUNT          117 a           80 c           70 g          119 t
ORIGIN      
        1 atgcgacgac aatggattta ttcttagcac aatcatcact cctagaaata ttcatgacag
       61 tcaagtcgcc tttcagctcg tcaaacaatc gaagagactt tttcctgaaa tcaattgtgt
      121 tgtcgctgat gctgggtata agacacctaa gtttgttcac tttttgactc atttgaacct
      181 tcgaccttgt ttgccttatt caaggcccaa agggaaaaag gagctattat caaaaaatga
      241 gtttctctat gacgagtatt ttgattgtta tatttgtccg caagatcaaa tgctcgcttt
      301 ttcaacggtt actcgagaag ggtaccgaga atacaaatcc aatccaaaag aatgtgtgaa
      361 ctgtcctctg ctaaaccaat gtacca
//
LOCUS       DAZKLM010000255          382 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3764
            SAMEA115706913-rid22582563.denovo.267, whole genome shotgun
            sequence.
ACCESSION   DAZKLM010000255 DAZKLM010000000
VERSION     DAZKLM010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706913
            Sequence Read Archive: ERR13196647
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 382)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 382)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287823.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287823.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,004
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,849
            CDSs (with protein)               :: 2,849
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 41 of 96
            Pseudo Genes (incomplete)         :: 52 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 15 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..382
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706913-rid22582563.denovo.267"
                     /isolate="VRE3764"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            166..382
                     /locus_tag="ACG842_002911"
                     /pseudo
     CDS             166..382
                     /locus_tag="ACG842_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
BASE COUNT          145 a           50 c           71 g          116 t
ORIGIN      
        1 taatcttctt tatagagaat tgggctattt ttaatggcaa agaggggaag aagatgtttc
       61 aaaacaaagg acaacgctat gttgtaaaag gagtaaagga atcttactgg aatcaaaaat
      121 ctttattgag aaaattatct ttgaatttaa gcacaaagaa atgaaggtac actagatagc
      181 aatgaagata tctttgtcat taaacgtcgc tattttacac aagtaagtga tgatttgatt
      241 catgtctttt ccaatgagtg gtttgtttct gagaaaaaat tacatgcttc taatctgcaa
      301 tatatgccag gtgaagatcc aattcctaat atgaaagcaa ttatcaacag taaggattac
      361 gaaggatata aagccaaaca tc
//
LOCUS       DAZKLM010000256          369 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3764
            SAMEA115706913-rid22582563.denovo.269, whole genome shotgun
            sequence.
ACCESSION   DAZKLM010000256 DAZKLM010000000
VERSION     DAZKLM010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706913
            Sequence Read Archive: ERR13196647
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 369)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 369)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287823.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287823.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,004
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,849
            CDSs (with protein)               :: 2,849
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 41 of 96
            Pseudo Genes (incomplete)         :: 52 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 15 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..369
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706913-rid22582563.denovo.269"
                     /isolate="VRE3764"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            98..>369
                     /locus_tag="ACG842_002912"
     CDS             98..>369
                     /locus_tag="ACG842_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0249338.1"
                     /translation="MINLGEWGAIAGSITAIVSLILLVIKPITASFSKITETLSKVSH
                     NLDLLTKDLESSKSDRLMIHEELKKHDERLDTHTEKLVEHTQQIKT"
BASE COUNT          139 a           52 c           70 g          108 t
ORIGIN      
        1 ttgtgcatta ctgaagaaga agcagataaa atcattagca aagaagagag cgcatcttaa
       61 ttgatgtgct ttttattttg attcaaggag ttgtcacatg attaatttag gggaatgggg
      121 agcgatagca ggatcaataa ccgctatcgt ttctttgatt ttattagtaa taaaaccaat
      181 tactgcatct ttctcgaaga ttactgagac tctttcaaaa gtaagtcaca atttagattt
      241 gctgactaaa gatttagaat cgagcaaatc agatcgattg atgattcatg aagaactaaa
      301 gaaacacgat gaaagattag atacacatac agaaaaattg gtggaacaca cgcaacaaat
      361 taaaacttt
//
LOCUS       DAZKLM010000257          368 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3764
            SAMEA115706913-rid22582563.denovo.270, whole genome shotgun
            sequence.
ACCESSION   DAZKLM010000257 DAZKLM010000000
VERSION     DAZKLM010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706913
            Sequence Read Archive: ERR13196647
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 368)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 368)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287823.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287823.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,004
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,849
            CDSs (with protein)               :: 2,849
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 41 of 96
            Pseudo Genes (incomplete)         :: 52 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 15 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..368
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706913-rid22582563.denovo.270"
                     /isolate="VRE3764"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..272)
                     /locus_tag="ACG842_002913"
     CDS             complement(<1..272)
                     /locus_tag="ACG842_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0249339.1"
                     /translation="MINLGEWGMIAGSITAIVSLILLVIRPIAASFSKITETLSKVSH
                     NLDLLTKDLESSKSDRLMIHEELKKHDERLDTHTEKLVEHTQQIKT"
BASE COUNT          106 a           69 c           52 g          141 t
ORIGIN      
        1 aaagttttga tttgttgcgt gtgttccacc aatttttctg tatgtgtatc taatctttca
       61 tcgtgtttct ttagttcttc atgaatcatc aatcgatctg atttgctcga ttctaaatct
      121 ttagtcagca aatctaaatt gtggcttact tttgaaagag tctcagtaat tttcgagaaa
      181 gatgcagcaa tcggtctaat tactaataaa atcaaagaaa cgatagcggt tattgatcct
      241 gctatcattc cccattcccc taaattaatc atgtgacaac tcctttacct taaataaaaa
      301 cgcatcaatt aagatgcgct cttatcttta ttaatgattt tatctgcttc ttcgtctgta
      361 atgcataa
//
LOCUS       DAZKLM010000258          368 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3764
            SAMEA115706913-rid22582563.denovo.271, whole genome shotgun
            sequence.
ACCESSION   DAZKLM010000258 DAZKLM010000000
VERSION     DAZKLM010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706913
            Sequence Read Archive: ERR13196647
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 368)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 368)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287823.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287823.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,004
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,849
            CDSs (with protein)               :: 2,849
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 41 of 96
            Pseudo Genes (incomplete)         :: 52 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 15 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..368
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706913-rid22582563.denovo.271"
                     /isolate="VRE3764"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            38..>368
                     /locus_tag="ACG842_002914"
     CDS             38..>368
                     /locus_tag="ACG842_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0249340.1"
                     /translation="MWLEKINDKKFKYTERYTDPLTEKKRKVSVTLSSNSRQAWNQAN
                     LLLNEKIAEKIKRNEELPLTFGELKTKWDEKYKPTVKESSYRTTQVYLSLISKYIKDD
                     VLVKNVNS"
BASE COUNT          168 a           49 c           56 g           95 t
ORIGIN      
        1 aattaatagg aacgtatatt cgaaaggaat agaaataatg tggttagaaa aaataaatga
       61 caaaaaattt aagtacaccg aacgttatac ggatccctta actgaaaaaa agagaaaagt
      121 gtccgttact ttatctagca attcacgcca agcttggaat caagctaact tacttttgaa
      181 tgaaaaaatc gcagaaaaaa taaaacgaaa tgaagaatta ccactaactt ttggagaatt
      241 aaaaactaaa tgggatgaaa aatacaagcc aactgtaaaa gaaagttcct atcgaacaac
      301 tcaggtttat ctatcattaa tttcaaaata tataaaagat gatgtacttg taaaaaacgt
      361 taatagta
//
LOCUS       DAZKLM010000259          362 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3764
            SAMEA115706913-rid22582563.denovo.272, whole genome shotgun
            sequence.
ACCESSION   DAZKLM010000259 DAZKLM010000000
VERSION     DAZKLM010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706913
            Sequence Read Archive: ERR13196647
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 362)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 362)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287823.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287823.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,004
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,849
            CDSs (with protein)               :: 2,849
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 41 of 96
            Pseudo Genes (incomplete)         :: 52 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 15 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..362
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706913-rid22582563.denovo.272"
                     /isolate="VRE3764"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..>362)
                     /locus_tag="ACG842_002915"
     rRNA            complement(<1..>362)
                     /locus_tag="ACG842_002915"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT           72 a          113 c           83 g           94 t
ORIGIN      
        1 aaatccggac aacgcttgcc acctacgtat taccgcggct gctggcacgt agttagccgt
       61 ggctttctgg ttagataccg tcaagggatg aacagttact ctcatccttg ttcttctcta
      121 acaacagagt tttacgatcc gaaaaccttc ttcactcacg cggcgttgct cggtcagact
      181 ttcgtccatt gccgaagatt ccctactgct gcctcccgta ggagtttggg ccgtgtctca
      241 gtcccaatgt ggccgatcac cctctcaggt cggctatgca tcgtggcctt ggtgagccgt
      301 tacctcacca actagctaat gcaccgcggg tccatccatc agcgacaccc gaaagcgcct
      361 tt
//
LOCUS       DAZKLM010000260          344 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3764
            SAMEA115706913-rid22582563.denovo.274, whole genome shotgun
            sequence.
ACCESSION   DAZKLM010000260 DAZKLM010000000
VERSION     DAZKLM010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706913
            Sequence Read Archive: ERR13196647
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 344)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 344)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287823.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287823.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,004
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,849
            CDSs (with protein)               :: 2,849
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 41 of 96
            Pseudo Genes (incomplete)         :: 52 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 15 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..344
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706913-rid22582563.denovo.274"
                     /isolate="VRE3764"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>344
                     /locus_tag="ACG842_002916"
     CDS             <1..>344
                     /locus_tag="ACG842_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
                     /protein_id="HFQ0249341.1"
                     /translation="YVRRFRETTVFEDIFAYILEQAVKAGFVTEDNLYLDSTHIKANA
                     NKHKFTKEMTHDEAKAYQDELEDEINRQRIEAGKRPFTLDLEKEVKLKERKISKADPE
                     SGYYVKGEREKQ"
BASE COUNT          132 a           54 c           77 g           81 t
ORIGIN      
        1 ttatgttcgc cgatttagag aaacgacggt gtttgaggat atttttgcct atattttaga
       61 acaagcagtt aaagcaggtt ttgtaacaga ggataatttg tatctggatt ctacccacat
      121 caaagctaac gccaataaac ataagtttac gaaagaaatg acccatgatg aagcgaaagc
      181 ctatcaggat gaattagaag atgagatcaa tcgtcaacga atagaggctg gaaaacgccc
      241 ttttactttg gacttagaaa aggaagtgaa gctgaaagaa agaaaaataa gtaaagctga
      301 tccggaaagc ggctactacg tcaaaggcga acgagaaaaa caat
//
LOCUS       DAZKLM010000261          323 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3764
            SAMEA115706913-rid22582563.denovo.275, whole genome shotgun
            sequence.
ACCESSION   DAZKLM010000261 DAZKLM010000000
VERSION     DAZKLM010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706913
            Sequence Read Archive: ERR13196647
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 323)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 323)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287823.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287823.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,004
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,849
            CDSs (with protein)               :: 2,849
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 41 of 96
            Pseudo Genes (incomplete)         :: 52 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 15 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..323
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706913-rid22582563.denovo.275"
                     /isolate="VRE3764"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..302)
                     /gene="tnpA"
                     /locus_tag="ACG842_002917"
     CDS             complement(<1..302)
                     /gene="tnpA"
                     /locus_tag="ACG842_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605-like element ISEfa4 family
                     transposase"
                     /protein_id="HFQ0249342.1"
                     /translation="MELDTNNHSVFLLYYHLVLVTKYRRQVIDDEISDYAKTTFERIS
                     ESYHITLVEWNHDKDHVHIMFKAQPKTELTKFINAYKSASSRLIKRDFPRVKQFLW"
BASE COUNT          100 a           49 c           51 g          123 t
ORIGIN      
        1 ttccaaagaa attgtttgac tcttggaaag tcacgtttta tcaatcttga gctggcactt
       61 ttataggcat tgatgaattt tgtcaattct gttttaggtt gagctttgaa cataatatga
      121 acgtggtctt tatcatgatt ccactccact aaagtaatgt gatacgattc tgaaattctt
      181 tcaaaagtag ttttggcata atcagatatt tcatcatcaa tcacttgtct acgatatttc
      241 gttactaaaa caaggtggta ataaagaaga aatactgaat gattatttgt atctaattcc
      301 atgttatatc aactcatttc ttg
//
LOCUS       DAZKLM010000262          321 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3764
            SAMEA115706913-rid22582563.denovo.276, whole genome shotgun
            sequence.
ACCESSION   DAZKLM010000262 DAZKLM010000000
VERSION     DAZKLM010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706913
            Sequence Read Archive: ERR13196647
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 321)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 321)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287823.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287823.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,004
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,849
            CDSs (with protein)               :: 2,849
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 41 of 96
            Pseudo Genes (incomplete)         :: 52 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 15 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..321
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706913-rid22582563.denovo.276"
                     /isolate="VRE3764"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>321
                     /locus_tag="ACG842_002918"
     CDS             <1..>321
                     /locus_tag="ACG842_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element ISS1N family transposase"
                     /protein_id="HFQ0249343.1"
                     /translation="TQAAYAFLKRLHKQFGEPKAIVTDKAPSLGSAFRKLQSVGLYTK
                     TEHRTVKYLNNLIEQDHRPIKRRNKFYQSLRTASSTIKGMETIRGIYKKNRRNGTLFG
                     FSVST"
BASE COUNT          107 a           69 c           63 g           82 t
ORIGIN      
        1 acgcaagcag cctatgcttt cttaaaacga ctccataaac agtttggtga gccgaaagca
       61 attgtgaccg ataaagcacc ttctcttggc tccgccttta gaaagttaca gagtgtgggt
      121 ttatatacta agacagagca ccgaactgtg aagtatctta acaatttaat agaacaagac
      181 catcgaccta ttaaacgacg aaataaattt tatcaaagtc tccgtacagc ctcttccacg
      241 attaagggca tggagaccat tcgaggaata tataaaaaga accgaagaaa tggaacgctc
      301 ttcggctttt cggtgtctac t
//
LOCUS       DAZKLM010000263          319 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3764
            SAMEA115706913-rid22582563.denovo.277, whole genome shotgun
            sequence.
ACCESSION   DAZKLM010000263 DAZKLM010000000
VERSION     DAZKLM010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706913
            Sequence Read Archive: ERR13196647
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 319)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 319)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287823.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287823.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,004
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,849
            CDSs (with protein)               :: 2,849
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 41 of 96
            Pseudo Genes (incomplete)         :: 52 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 15 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..319
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706913-rid22582563.denovo.277"
                     /isolate="VRE3764"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            20..>319
                     /locus_tag="ACG842_002919"
     CDS             20..>319
                     /locus_tag="ACG842_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6056 family protein"
                     /protein_id="HFQ0249344.1"
                     /translation="MNKHNRKITNNKELILLLIGIWGIFLLFNMYMPTMRADDVVYAS
                     RLDELGYLGASIEHYKTWSSRIIIELFLMFFSKHFMLWKLLNSTIMLGIVVLLCKY"
BASE COUNT          115 a           34 c           59 g          111 t
ORIGIN      
        1 tataagaagg agtataaaaa tgaacaaaca taatagaaaa ataacgaata acaaagaact
       61 gatcttgtta ctaataggga tttggggtat ctttttactc tttaatatgt atatgccgac
      121 tatgagagcg gatgacgtag tttatgcaag tagattagat gaacttggct atttgggtgc
      181 ttcgatagaa cattataaga catggagttc aagaattatt atagaactat tcttaatgtt
      241 cttttctaaa cactttatgc tatggaaatt attaaattct acgattatgt taggaattgt
      301 agtattactt tgcaagtat
//
LOCUS       DAZKLM010000264          315 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3764
            SAMEA115706913-rid22582563.denovo.278, whole genome shotgun
            sequence.
ACCESSION   DAZKLM010000264 DAZKLM010000000
VERSION     DAZKLM010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706913
            Sequence Read Archive: ERR13196647
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 315)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 315)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287823.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287823.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,004
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,849
            CDSs (with protein)               :: 2,849
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 41 of 96
            Pseudo Genes (incomplete)         :: 52 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 15 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..315
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706913-rid22582563.denovo.278"
                     /isolate="VRE3764"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>315
                     /locus_tag="ACG842_002920"
     CDS             <1..>315
                     /locus_tag="ACG842_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0249345.1"
                     /translation="HWNAIEGKPTTVKGDKGDPGQAATITIGTVSSGSTASVTNVGTS
                     SAARFNFVLPKGDKGDPGINATTTAVATTTANGLMSSTDKTKLDGIAAGAQKNPGNAT
                     TTT"
BASE COUNT          109 a           62 c           72 g           72 t
ORIGIN      
        1 tcattggaat gctatagaag gcaaaccaac aacggtgaag ggagataaag gcgatcctgg
       61 ccaagcagca acaattacca taggaaccgt ttctagtggt tcaaccgctt ctgttacgaa
      121 tgtaggaaca tcatctgcag ctagatttaa ttttgtttta ccaaagggag ataaaggtga
      181 tcctggaata aatgccacaa ctacagctgt agctacaact acagcaaatg ggttgatgtc
      241 gtccacagat aaaactaagt tggatggaat tgcagctgga gcacagaaaa atccaggtaa
      301 tgctacaact acgac
//
LOCUS       DAZKLM010000265          296 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3764
            SAMEA115706913-rid22582563.denovo.280, whole genome shotgun
            sequence.
ACCESSION   DAZKLM010000265 DAZKLM010000000
VERSION     DAZKLM010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706913
            Sequence Read Archive: ERR13196647
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 296)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 296)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287823.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287823.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,004
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,849
            CDSs (with protein)               :: 2,849
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 41 of 96
            Pseudo Genes (incomplete)         :: 52 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 15 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..296
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706913-rid22582563.denovo.280"
                     /isolate="VRE3764"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>296
                     /locus_tag="ACG842_002921"
     CDS             <1..>296
                     /locus_tag="ACG842_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0249346.1"
                     /translation="LTHNFRDNEFDCNIELVKKIEGDLSYILENEIFVNKTRRLLLGR
                     CRTCIQKAVRYASFNKEFDLNSYLSKILMNDELIACIHSYPYKQNPIKQRIFNY"
BASE COUNT          112 a           35 c           47 g          102 t
ORIGIN      
        1 ttgacacata attttagaga taatgagttc gattgtaata ttgaattagt taaaaaaatt
       61 gaaggcgatt taagctatat attagaaaat gagatatttg taaacaaaac aaggagactt
      121 ttgttgggac gctgtcgtac ttgtattcaa aaggcagtta gatatgctag ttttaataaa
      181 gaatttgatt taaactccta tttgtctaag atacttatga acgatgagtt gattgcctgt
      241 attcactcat acccatataa acaaaatcca attaaacaac gaatatttaa ctattt
//
LOCUS       DAZKLM010000266          291 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3764
            SAMEA115706913-rid22582563.denovo.281, whole genome shotgun
            sequence.
ACCESSION   DAZKLM010000266 DAZKLM010000000
VERSION     DAZKLM010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706913
            Sequence Read Archive: ERR13196647
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 291)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 291)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287823.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287823.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,004
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,849
            CDSs (with protein)               :: 2,849
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 41 of 96
            Pseudo Genes (incomplete)         :: 52 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 15 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..291
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706913-rid22582563.denovo.281"
                     /isolate="VRE3764"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>291
                     /locus_tag="ACG842_002922"
     CDS             <1..>291
                     /locus_tag="ACG842_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_064305258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IS6-like element IS1216 family transposase"
                     /protein_id="HFQ0249347.1"
                     /translation="QQDVIIVAVGYYLRYNLSYREVQEILYDRGINVSHTTIYRWVQE
                     YGKLLYQIWKKKNKKSFYSWKMDETYIKIKGKWHYLYRAIDADGLTLDIWLR"
BASE COUNT           98 a           43 c           58 g           92 t
ORIGIN      
        1 tcagcaggat gtgattattg tagccgtggg ctactatctt cgttataacc ttagctatcg
       61 tgaagttcaa gaaatcttat atgatcgtgg cattaacgtt tctcatacga cgatttatcg
      121 ttgggtgcaa gaatatggca aactactcta tcaaatttgg aaaaagaaaa ataaaaaatc
      181 cttttattca tggaaaatgg atgaaacgta catcaaaatt aaaggaaaat ggcattattt
      241 gtatcgagcc atcgatgcag atggtttaac cttggatatt tggttacgta a
//
LOCUS       DAZKLM010000267          279 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3764
            SAMEA115706913-rid22582563.denovo.284, whole genome shotgun
            sequence.
ACCESSION   DAZKLM010000267 DAZKLM010000000
VERSION     DAZKLM010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706913
            Sequence Read Archive: ERR13196647
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 279)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 279)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287823.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287823.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,004
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,849
            CDSs (with protein)               :: 2,849
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 41 of 96
            Pseudo Genes (incomplete)         :: 52 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 15 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..279
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706913-rid22582563.denovo.284"
                     /isolate="VRE3764"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            1..279
                     /locus_tag="ACG842_002923"
                     /pseudo
     CDS             1..279
                     /locus_tag="ACG842_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          107 a           56 c           55 g           61 t
ORIGIN      
        1 caaatcgtcg atctctatct cgctggtaag cctcgcgcag aaattattcg agagtatgag
       61 cttacgcctt cttctttcga taaatggatg aagcaagcac aatcaacggg ctcattcaaa
      121 gaaagagaca acttaacacc agaacaagca gaattgatcg cactaagaaa gaaaaataag
      181 caactcgaaa tggagaatga tattttaaag caagcggcgc tgatattcgg acgaaaagac
      241 aagtaattga tgccaacaag cataaatatt ccatatcag
//
LOCUS       DAZKLM010000268        22133 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3764
            SAMEA115706913-rid22582563.guided.050, whole genome shotgun
            sequence.
ACCESSION   DAZKLM010000268 DAZKLM010000000
VERSION     DAZKLM010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706913
            Sequence Read Archive: ERR13196647
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22133)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22133)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287823.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287823.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,004
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,849
            CDSs (with protein)               :: 2,849
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 41 of 96
            Pseudo Genes (incomplete)         :: 52 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 15 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22133
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706913-rid22582563.guided.050"
                     /isolate="VRE3764"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(165..1100)
                     /gene="whiA"
                     /locus_tag="ACG842_002924"
     CDS             complement(165..1100)
                     /gene="whiA"
                     /locus_tag="ACG842_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0043937 - regulation of sporulation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HFQ0249348.1"
                     /translation="MSFAADVKKELTGLEVHREHAKAELAALLRMNGSLGIVNQQFIL
                     NVQTENAAIARRIYSLLKDHYQVRSELLVRKKMKLKKNNVYIVRLKQGTKDVLMDLDI
                     MDGMMFNGHVSDEIMGNSQKMRSYLRGAFMASGSVNNPETSRYHLEIFSIYEQHNQDI
                     CDMLNYYGLHARTLERRNGYISYLKGAEKIADFLTLIGATNSMLRFEDVRIVRDMRNS
                     VNRLVNCETANLNKTIDAASKQIENIELIEAKVGLHALPEKLQEIAELRLQHPEVSLK
                     ELGEMIPSGAISKSGINHRIRKINEFAEKLREEAV"
     gene            complement(1134..2132)
                     /gene="yvcK"
                     /locus_tag="ACG842_002925"
     CDS             complement(1134..2132)
                     /gene="yvcK"
                     /locus_tag="ACG842_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine diphosphate-N-acetylglucosamine-binding
                     protein YvcK"
                     /protein_id="HFQ0249349.1"
                     /translation="MKMKTYRIRKPKIVVVGGGTGLPVILKSLRNQGADITAVVTVAD
                     DGGSSGAIRESIAMAPPGDLRNVLVALSDMPQFYEDIFQYRFKKEDQFLANHTIGNLI
                     IAAVSEMRGSTYEAIQLLAKMMHVDGHVYPSSETPLTLHAVFKDGTTAVGESKIAINR
                     KTIDRVYVCNTNDKSEAKAARKVVSAIMDADMVVLGPGSLFTSILPNLVIPEIGEAML
                     KTTAETVYICNIMTQKGETEHFTDADHIRVLHKHLNQPFIDTVLVNTEKVPEDYMDPE
                     IYDEYLVQVAHDFNGLRNEGCRVISTDFLELKDGGVFHDGDKVVEELFRLVFGSKY"
     gene            complement(2129..3013)
                     /gene="rapZ"
                     /locus_tag="ACG842_002926"
     CDS             complement(2129..3013)
                     /gene="rapZ"
                     /locus_tag="ACG842_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HFQ0249350.1"
                     /translation="MTDNLQLVIITGMSGAGKTVAIQSFEDMGYFCIDNMPPRLIPKF
                     WELIKESGKVTKMALGVDLRSRSFFEEIQNMLIEIENTNFIDTSILFLDASDEELVAR
                     YKETRRAHPMAMDGLITEGIRKERAILDDLKAQASVIIDTTTLTPRQLREKINESFKS
                     KGDQGFRIEFVSFGFKYGLPIDSDIVMDVRFLPNPHYIPELRPLTGKDQPVYDYVMSF
                     DETQSFYHKFLDLLETVLPGYIKEGKSSLTVAIGCTGGQHRSVALTERLANALGKNYK
                     VNVTHRDKDKRKETVNRS"
     gene            complement(3148..3879)
                     /locus_tag="ACG842_002927"
     CDS             complement(3148..3879)
                     /locus_tag="ACG842_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate racemase"
                     /protein_id="HFQ0249351.1"
                     /translation="MENFFSILGGMGTMATESFVRLINHRTKATKDQEYLNYVLFNHA
                     TVPDRTAYILDRSEENPMPFLLDDIEKQNLLRPNFIVLTCNTAHYFFEELQAATDIPI
                     LHMPREAANELVRQHTTGRVAILGTEGSMKAGIYEREVKNLGFETVIPDTALQEKINY
                     LIYHEIKESDYLNQELYYEILEEAVERLNCEKVILGCTELSLMHEFAEDNHYPVIDAQ
                     SILADRTIERALAERSEALDTASEK"
     gene            complement(3883..5148)
                     /locus_tag="ACG842_002928"
     CDS             complement(3883..5148)
                     /locus_tag="ACG842_002928"
                     /EC_number="6.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aspartate ligase"
                     /protein_id="HFQ0249352.1"
                     /translation="MMNSIENEEFIPILLGSDMNVYGMARSFNEAYGKICQAYASDQL
                     APTRYSKIVNVEVIPGFDKDPVFIETMLRLAKERYSDKSKKYLLIACGDGYAELISQH
                     KQELSEYFICPYIDYSLFERLINKVSFYEVCEEYDLPYPKTLIVREEMLVNGHLEQEL
                     PFEFPVALKPANSVEYLSVQFEGRKKAFILETREEFDLILGRIYEAGYKSEMIVQDFI
                     PGDDSNMRVLNAYVDEDHQVRMMCLGHPLLEDPTPASIGNYVVIMPDYNEKIYQTIKA
                     FLEKIEYTGFANFDMKYDPRDGEYKLFEINLRQGRSSFFVTLNGLNLARFVTEDRVFN
                     KPFVETTYGTNQSDKARLWMGVPKKIFLEYARENEDKKLAEQMIKENRYGTTVFYEKD
                     RSIKRWLLMKYMFHNYIPRFKKYFHVKEG"
     gene            complement(5411..8227)
                     /gene="uvrA"
                     /locus_tag="ACG842_002929"
     CDS             complement(5411..8227)
                     /gene="uvrA"
                     /locus_tag="ACG842_002929"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HFQ0249353.1"
                     /translation="MANDKITIHGARAHNLKNIDVTIPRDKFVVVTGLSGSGKSSLAF
                     DTLYAEGQRRYVESLSAYARQFLGQMDKPDVDSIDGLSPAISIDQKTTSKNPRSTVGT
                     VTEINDYLRLLFARVGHPICPNDHIEITSQSVDQMADQVMALPERTKIQVLAPIVVKK
                     KGQHKKIFERIQKEGYVRVRVDGEIYDLSEVPELEKNKKHDIAIVIDRIIVKEGIRSR
                     LFDSLEAALRLADGYALVDVIGEEEMLFSEHYACPYCGFTVGELEPRLFSFNAPFGAC
                     PDCDGLGVKLEVDQDLVIPDRTKTLSQGAIVPWNPISSQYYPQMLAQACESFGIDLDT
                     PFEELPEEHQEIILNGTDDLFHFHYENDFGGVRDVETTFEGVLKNIERRYHDTNSDFT
                     REQMRLYMTELTCQTCKGYRLNPQALSVQINGTNIGQTNELSIQKAVDFFSNLTLSEQ
                     EKVIAKPILKEVNDRLSFLENVGLDYLTLSRASGTLSGGEAQRIRLATQIGSNLSGVL
                     YILDEPSIGLHQRDNDRLIGSLKKMRDLGNTLVVVEHDEDTMWAADYLIDVGPGAGEQ
                     GGQIVAAGTPEEVANDENSLTGKYLSGKKSIPVPKERRKGNGKAIKITGASENNLKNI
                     SVEFPLGEFVAVTGVSGSGKSTLVNSILKKSLAQKLNKNSAKPGKFKTISGYESIEKI
                     IDIDQSPIGRTPRSNPATYTSVFDDIRGLFAQTNEAKMRGYKKGRFSFNVKGGRCEAC
                     RGDGIIKIEMHFLPDVYVPCEVCHGKRYNSETLEVHYKGKSIADILEMTVEDAVEFFK
                     HIPKIHRKLQTIVDVGLGYVTMGQPATTLSGGEAQRMKLASELHKISNGKNFYILDEP
                     TTGLHSDDIARLLHVLQRLVDAGNTVLVIEHNLDVIKTADYIIDLGPEGGEGGGTILA
                     TGTPEEIINVKESYTGHYLKKIMV"
     gene            complement(8237..10231)
                     /gene="uvrB"
                     /locus_tag="ACG842_002930"
     CDS             complement(8237..10231)
                     /gene="uvrB"
                     /locus_tag="ACG842_002930"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HFQ0249354.1"
                     /translation="MIERDTSRHFELVSKYQPAGDQPEAINQLVDGVVGGKKAQILLG
                     ATGTGKTYTISNLIEKVNKPTLIIAHNKTLAGQLYGEFKEFFPNNAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVNDEIDKLRHSATSSLLERNDVIVIASVSCIFGLGSPFEYQ
                     KQVVSIRQGAELDRNQLIRDLVSIQFERNDIDFQRGRFRVRGDVVEIFPASRDERALR
                     VEFFGDEVERIREVNALTGEVLGETEHVAIFPATHFVTNDEHMEHAVANIKEELEQRL
                     TVLRNENKLLEAQRLEQRTNYDIEMMLEMGYTSGIENYSRHMDGRKEGEPPYTLLDFF
                     PEDFLIVADESHVTMPQIRGMYNGDRARKQMLVDYGFRLPSALDNRPLRLEEFEKHVN
                     QIIYVSATPGPYEHEQTDTVIQQIIRPTGLLDPVIEVRPIMGQIDDLVGEINERVEKD
                     QRVFVTTLTKKMAEDLTDYFKELGIKVKYLHSDIKTLERTEIIRDLRLGEFDVLVGIN
                     LLREGLDVPEVSLVAILDADKEGFLRSERSLVQTIGRAARNEEGKVIMYADKVTDSMR
                     LAMDETSRRRTIQQKYNEEHGIVPKTIIKEIRDLISITKESEDDTKEAVQVSYEEMTK
                     EEKDTLLMKLEKEMKDAAKALDFETAANVRDMILELKASK"
     gene            10490..11992
                     /locus_tag="ACG842_002931"
     CDS             10490..11992
                     /locus_tag="ACG842_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HFQ0249355.1"
                     /translation="MKRKHLFHSFVFLMTGLVTLAFGHVAQADNTTTAESSTSETMQK
                     IEPKKDVYVIASDSAFAPFEFQNAKGEYEGIDVKLMENIAKLQGFNIRMDYRGFSAAL
                     QALESGQADGMIAGMTVTDERKKSYDFSDSYFDSGIQLAVKKGDTSIKSYEDLKGKTV
                     GAKVGTESAEFLSDHESEYGYSIKYFDAADQLYDGLKVGAIDAMMDDYPVIGYAIKQG
                     QELETPIKKESGGKYAFAVKKGQNPELLNMFNEGLKELKRTGEYDKIIKEYVADGSET
                     SSESSTADESTFLGLIQNNYQALLKGLWRTILLTLISFALAIVIGVIFGLFSVAPIRA
                     LRTIASIYVDIIRGIPMMVLAFFIFFGLPGIVGFTIPDFLAGVITLTLNASAYIAEIV
                     RGGINAVPVGQMEASRSLGLSYNRTMQKIILPQAIRIMIPSFVNQFVISLKDTTIISA
                     IGVVELLQTGKIIVARTTQSTYVYLIIAIMYLILITALTKLAKVLEKKVK"
     gene            11994..12731
                     /locus_tag="ACG842_002932"
     CDS             11994..12731
                     /locus_tag="ACG842_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFQ0249356.1"
                     /translation="MAEKILVEHLVKKYGDNTVLNDINVSINEGDVVCIIGPSGSGKS
                     TFLRCLNRLEEPSDGEIIIDGAHLMDKSTDINLVRQHIGMVFQHFNLFPHLSILENII
                     LAPMDLKKESREEAEKKAIKLLQTVGLEDKKDMYPEMLSGGQKQRVAIARALAMNPDI
                     MLFDEPTSALDPEMVGDVLNVMKKLAEQGMTMVIVTHEMGFAKEVANRVMFIDDGNFL
                     EDGTPQQIFENPQNERTKDFLNKVLNI"
     gene            complement(12902..14095)
                     /locus_tag="ACG842_002933"
     CDS             complement(12902..14095)
                     /locus_tag="ACG842_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005336078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFQ0249357.1"
                     /translation="MKEKRRDNKGRILHTGESQRTDRKYLYKYVDAFGNTKYVYAWRL
                     TPTDPTPKGKREKPSLRELEQQIRRDIEDGIDSTGKKMTLCQLYAKQNAQRANVKKST
                     QKQREQLMRLLKEDKLGARSIDTIKPSDAKEWALRMKDKGFSYNTINNHKRSLKASFY
                     IAIQDDYVRKNPFDFKLSEVLENDTKKKVALTEEQEQALLSFIKTDNVYHKHYDDVLI
                     LLKTGLRISELCGLTVADIDFKNEVVIIDHQLLKSKEQGYYIETPKTKSGIRQVPLSK
                     ETIQAFQQVMKKRPKAEPFVIDGRGNFLFVNHKGKPKVAIDYNALFIRMVKKYNKHHK
                     DNPLPHITPHTLRHTFCTRLASKNMNPKDLQYIMGHSNISITMNWYAHASIDTAKSEV
                     QRLIA"
     gene            complement(14174..14380)
                     /locus_tag="ACG842_002934"
     CDS             complement(14174..14380)
                     /locus_tag="ACG842_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HFQ0249358.1"
                     /translation="MSNAQDIPVWEKYTLTIEEASKYFRIGENKLRRLAEENKDAGWL
                     IMNGNRIQIKRRQFEQVIDKLDAI"
     gene            complement(14373..15515)
                     /locus_tag="ACG842_002935"
     CDS             complement(14373..15515)
                     /locus_tag="ACG842_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005336080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HFQ0249359.1"
                     /translation="MNNLEFALEMKNKRLASGLSQGELASLTHVSRYSINRFENGKAN
                     ASKETQNIILRCLNYYICDKPFYLLVDYLSVRYPTTDALEIIRKVLGMKADYFIHYDY
                     GYYGYKEHYAYGEIKVMASDDEHMGVFLELKGAGSRNMEYVLQAQNRDWYSFLNRCLD
                     CDGVIRRFDLAINDMCGLLDIPVLSEKYRNGGADCRCKNYENVQGGKLSGKNRNLAST
                     LYIGSKASTKYFCLYEKQKEQATKKKHTDIINRFEIRLRGTKAVQAVEELLLTYNPHG
                     LVFYLITDFVQFPDYPLWEIFISHDSLPFEMNPVPVNMERTLQWLERQVMPSIVMIEE
                     IDRLTGSNYMKMIDECTHLSEKQEMLVEQMCTDIADVIESEGVFYE"
     gene            16027..16662
                     /locus_tag="ACG842_002936"
     CDS             16027..16662
                     /locus_tag="ACG842_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFQ0249360.1"
                     /translation="MEGYAMKISHLGNNIQTIRKFRGMKQQELADKIGINMQSLSKIE
                     RGLNYPAYETLEKIMEVLDVTPNELLSGEWKYVNQSEKEVCQFLRAEERLNAELKHGH
                     YDNFFDSEEEWLEYELEKLREYITDYINGKSIEASDLYPIKEFIQHLKFQKLLDRYDD
                     LYSMDMFGESIEGHKYKTPYQVVKMINPNSKEDMELLREVLRNNNFDDEDE"
     gene            16816..16893
                     /locus_tag="ACG842_002937"
                     /pseudo
     CDS             16816..16893
                     /locus_tag="ACG842_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345006.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
     gene            17207..17254
                     /locus_tag="ACG842_002938"
                     /pseudo
     CDS             17207..17254
                     /locus_tag="ACG842_002938"
                     /inference="COORDINATES: protein motif:HMM:NF011335.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            17270..19189
                     /gene="tet(M)"
                     /locus_tag="ACG842_002939"
     CDS             17270..19189
                     /gene="tet(M)"
                     /locus_tag="ACG842_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HFQ0249361.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLFPLYH
                     GSAKSNIGIDNLIEVITNKFYSSTHRGPSELCGNVFKIEYTKKRQRLAYIRLYSGVLH
                     LRDSVRVSEKEKIKVTEMYTSINGELCKIDRAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRKKIENPHPLLQTTVEPSKPEQREMLLDALLEISDSDPLLRYYVDSTTHEIILSFLG
                     KVQMEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVS
                     PLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAPQEYLSRAYNDAPKYCANIV
                     DTQLKNNEVILSGEIPARCIQEYRSDLTFFTNGRSVCLTELKGYHVTTGEPVCQPRRP
                     NSRIDKVRYMFNKIT"
     gene            19308..19475
                     /locus_tag="ACG842_002940"
     CDS             19308..19475
                     /locus_tag="ACG842_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich KTR domain-containing protein"
                     /protein_id="HFQ0249362.1"
                     /translation="MCPVCGNKTRLKIREDTELKKFPLYCPKCRQENLIEIKQFKVTV
                     ITEPDAKTQSR"
     gene            complement(19535..19888)
                     /locus_tag="ACG842_002941"
     CDS             complement(19535..19888)
                     /locus_tag="ACG842_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0249363.1"
                     /translation="MRKKEDKYDFRAFGLAIKEARLKRGLTREQVGALIEIDPRYLTN
                     IENKGQHPSIQVLYDLVSLLHVSVDEFFLPANNLVKSTRRLQIEKYMDSFTDKELSLM
                     ESLASGINEARNIED"
     gene            20392..20811
                     /locus_tag="ACG842_002942"
     CDS             20392..20811
                     /locus_tag="ACG842_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009588541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HFQ0249364.1"
                     /translation="MRTSSFEHIVRLQFNSLMLIVIKNKVKSRNRQLARHSKREVLFC
                     EIAETKQVERGTIDTCFYDCVSFKVLHFTIYVSDETLGTVLHRLSEKQRSAILLRYFQ
                     GMNDREISELYHVSRSAICSRRTRGLRKLKMLLSERK"
     gene            20837..21043
                     /locus_tag="ACG842_002943"
     CDS             20837..21043
                     /locus_tag="ACG842_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004613734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0249365.1"
                     /translation="MICKASSGDEMAVKEILKFYDGYISKLCLRPFYHSESGKIIMQV
                     DEELKGEIHTDMMKAILKFEIGVK"
     gene            complement(21271..21444)
                     /locus_tag="ACG842_002944"
     CDS             complement(21271..21444)
                     /locus_tag="ACG842_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0249366.1"
                     /translation="MEKKNQAQLSEGGIDYVLDIINEPMVLSYAMEQVERLLVCAGKQ
                     AMRLIRLGEKIRR"
     gene            21456..22085
                     /locus_tag="ACG842_002945"
     CDS             21456..22085
                     /locus_tag="ACG842_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0249367.1"
                     /translation="MGQLSDNQQLCQERINPLLELLERVFSYYGQALLTVHRQQIIIL
                     VNRISRASLLSLLDKIQTKFNKMYQLQLNFGIGSLCYTEQETPQSFLHAKQVCEWIAF
                     HQSVNEIRFFEDLDLGIVLPAIPSDQRTLYVKRILKSLTEEEVHLFKKTLACFSKNNG
                     SIKNCSEELFIHKNTLQYRLNKFHSLTGYTPRNYDDYHILKLAFLLVQT"
BASE COUNT         6523 a         4425 c         4044 g         7141 t
ORIGIN      
        1 cccgcttttt atacgattgt attaaaaaac aatagaagcc tttcaacatt atgttactcc
       61 agtaaagttg gaattatagt ttgacggaag aaaagcaagt atagctttta aaaaaaactg
      121 atacattcgc atctcctgct tcatgtatca gttttttctt tggattaaac tgcttcttca
      181 cgcagttttt ccgcaaattc atttattttg cgaattcggt gattaattcc cgattttgat
      241 attgcacctg agggaatcat ttcccctaat tctttcaagc tgacctctgg atgttgaagc
      301 cgaagttctg cgatttcctg aagtttttca ggaagggcat gtagaccgac ctttgcttcg
      361 atcaattcga tgttttcgat ttgtttagaa gccgcatcaa tcgtcttgtt cagatttgct
      421 gtctcacaat tgactagacg attgaccgaa tttctcatat cccgtacgat tcgaacatct
      481 tcaaatcgga gcattgagtt ggttgcacca atcaatgtta agaaatcagc aattttttct
      541 gctcctttta aatacgaaat atagccattg cgccgttcaa gcgttcgagc atgtagaccg
      601 taataattta acatatcgca aatgtcttgg ttatgctgtt cataaataga aaatatttcc
      661 aaatgataac gactcgtttc cggattattg actgaacctg aggccataaa agcaccacgt
      721 aagtaagaac gcattttttg cgaatttccc ataatctcgt ccgaaacgtg tccattgaac
      781 atcataccat ccatgatatc aagatccatc aacacatctt ttgtgccttg cttcaaccgg
      841 acgatataga cattgttctt ttttaatttc atttttttac gaacgagtag ctcacttcgt
      901 acctgataat gatctttcaa aagagaatag atacgtctgg caattgcagc attttctgtt
      961 tggacgttca aaataaactg ctggttcaca atacctaaag agccgttcat tcgcagcaaa
     1021 gcagctaatt ccgctttcgc atgttctcga tgaacttcca aaccagttag ttctttcttc
     1081 acatctgcag caaatgacat cctattctcc tcctttctcc aacaataaat tgcttaatat
     1141 tttgaaccga aaactaaacg gaacaattct tctactactt tgtctccatc gtggaagaca
     1201 ccaccatctt tcaattctaa aaagtcggtg gaaatcactc gacatccttc atttctcaat
     1261 ccgttgaagt cgtgtgcgac ttgtactaga tactcatcat agatctctgg gtccatataa
     1321 tcttcaggca ctttttccgt attgaccaat actgtatcga taaaaggctg attcaaatgc
     1381 ttgtgtaaaa cacgtatatg atcggcatct gtgaaatgtt ccgtttcccc tttttgggtc
     1441 atgatattac agatatagac ggtttcagca gttgtcttga gcattgcttc gccgatttct
     1501 ggtatcacta aatttggaag gatactggtg aataaacttc cgggtcccaa aacgaccatg
     1561 tcggcgtcca taattgctga aacgactttt cgagccgctt tggcttcact tttatcattt
     1621 gtgttgcaga catacacacg gtcaatggtt ttacggttga tcgcgatttt agattctcct
     1681 actgcagtcg ttccgtcttt aaaaacagca tgcaaggtca aaggagtttc tgaagaagga
     1741 taaacatgtc catctacatg catcattttt gctaataatt gaatcgcctc ataagtgctt
     1801 ccccgcatct cggaaactgc ggcaatgatc agattaccga tcgtatgatt ggctaaaaac
     1861 tgatcttcct ttttgaaacg gtattggaaa atatcttcat agaattgtgg catatccgat
     1921 aacgccacta agacatttct caagtcacca ggaggagcca tagcgatcga ttctctgatt
     1981 gcaccgctgc ttccaccatc atctgcaaca gtcactactg ctgtgatatc tgctccttga
     2041 tttcgtaaac ttttcaggat aactggcaga ccagtgccac cgccgaccac tacgatcttt
     2101 ggttttctta tccggtaggt tttcattttc acgaccggtt caccgtctct ttccttttat
     2161 ccttgtcccg atgtgtcaca ttgactttat agtttttccc taaagcattt gccaaacgtt
     2221 cagtcaatgc cacagaacgg tgttgaccgc ctgtacagcc gattgcaact gtcaagctgc
     2281 tttttccttc tttaatgtat ccaggcaata cagtttccag aagatccaaa aatttatggt
     2341 agaatgattg cgtttcatca aaggacatca cataatcata gactggctga tcttttccgg
     2401 tcaatggtct tagttccggg atataatgag ggttgggcaa aaaacgcaca tccatcacaa
     2461 tatccgaatc gatcggcaac ccgtatttaa aaccaaatga tacaaattcg atccgaaatc
     2521 cttggtctcc ctttgatttg aatgactcat tgattttttc acgtaattgt cgtggtgtca
     2581 atgttgtcgt atcaatgatc acggaagctt gggctttcaa gtcgtctagt atcgcccgtt
     2641 ctttacgtat cccttcagtg atcaaaccgt ccatcgccat tggatgtgct cgtcttgttt
     2701 ctttgtagcg agccactagt tcttcgtctg aagcatctaa aaataagata gaagtgtcga
     2761 tgaagttcgt attttcgatt tcgatcagca tgttttggat ttcttcaaag aaggaacgag
     2821 aacgcaaatc cactcctaaa gccatttttg tgactttacc tgattctttg atcaattccc
     2881 agaattttgg aatcaatcga ggcggcatat tatcgataca aaaatagccc atatcttcaa
     2941 aactttggat agctactgtt ttccctgcac cactcatacc tgttatgatg actaattgca
     3001 aattatctgt catatttacg aacccctctc acattgtgca taataaaatt atatcatccc
     3061 gggcgtatca ttaccagttt tctttttctt ttacaaaaag atttggacca aatatctttt
     3121 aggatatttg ccccaaatcc aattttttta cttttccgat gctgtatcca atgcctcgct
     3181 tctttcagct aatgcacgtt cgatcgttcg atccgctaaa atagattgag catcgatcac
     3241 tgggtaatga ttatcttcag caaattcatg catcaaagat aattctgtgc aacctaggat
     3301 gactttttca cagtttaggc gctctaccgc ttcctctaag atttcgtaat agagctcttg
     3361 attcaaataa tccgattcct tgatctcatg atagatcagg taattgattt tttcttgaag
     3421 cgcagtatca ggaatcactg tttcgaaacc aagatttttg acttcgcgtt catagattcc
     3481 agctttcata cttccttctg ttcctagaat cgctactctc cctgtcgtat gctgacggac
     3541 aagttcattt gctgcttctc ttggcatatg caagatagga atatccgttg ccgcttgtaa
     3601 ctcttcaaaa aagtagtgtg cggtgttaca agtcaacaca atgaagtttg gtcgcaataa
     3661 attttgtttc tcaatatcat ccagcagaaa cggcattgga ttttcttctg atcgatctaa
     3721 aatataagct gtacgatcag gaactgttgc atgattgaat aagacataat tcaaatattc
     3781 ttgatctttt gtagctttcg ttcggtggtt gatcaagcga acaaaacttt ctgtcgccat
     3841 cgtgcccatt ccgcctaaaa tactgaaaaa attctccatg aactagcctt ctttcacatg
     3901 aaaatacttt ttgaatcttg gaatatagtt atggaacata tatttcatca acaaccagcg
     3961 cttgattgat cggtcttttt cataaaatac agtagtaccg taacgatttt ctttgatcat
     4021 ttgctcagct agttttttgt cttcattttc ccgagcatat tctaaaaaga tttttttggg
     4081 cacacccatc cataatcttg ctttatctga ttgattcgtt ccataggtcg tttcaacaaa
     4141 gggtttgttg aacactcgat cttctgtgac aaaacgagcc agattcaagc cgttcaatgt
     4201 cacaaagaaa ctgctacgac cttgtctcaa attgatttca aataatttat attctccatc
     4261 tctaggatca tacttcatat cgaaattggc aaagcctgta tattcgattt tttccaaaaa
     4321 tgccttaatg gtttgataga ttttttcgtt ataatctggc atgatcacaa cgtaattacc
     4381 aatcgatgct ggtgtaggat cttctaatag tggatgacca aggcacatca tccgaacttg
     4441 atgatcttca tctacatacg catttagcac acgcatatta ctatcatcac ccgggataaa
     4501 atcttgtaca atcatttcac ttttataacc ggcttcgtat atccggccta aaattaaatc
     4561 gaactcttct ctagtctcta aaataaaagc tttttttctt ccttcaaatt ggacagaaag
     4621 atactccaca ctgtttgcag gtttcaaagc aactgggaat tcaaaaggca attcctgttc
     4681 caaatgtcca tttactaaca tttcttctct gacgatcaat gttttaggat acggaagatc
     4741 atattcttca caaacttcat aaaagcttac tttattgatc agcctttcaa ataaagaata
     4801 atcaatatat ggacagataa aatattctga cagctcttgt ttatgttggg agatcaattc
     4861 tgcatatcca tcaccacaag caatcaaaag atatttttta ctcttatcag aatatctttc
     4921 ttttgccaaa cgaagcatcg tctcgataaa tacagggtct ttatcaaatc ctggaataac
     4981 ttctacattg acaattttac tgtagcgggt aggggctaac tgatccgaag catatgcttg
     5041 gcagattttg ccgtatgctt cattaaaaga acgtgccata ccataaacgt tcatgtcgct
     5101 tcctaataat atagggataa attcttcatt ttcaatactg ttcatcatta tctctcactt
     5161 actcataatc tcttgttttt cttttccaac tacacaagga atatcatacc ataaagaaaa
     5221 aagcaatcaa aggaaagtcc tccgattgct caattctttt ttgaacggat tgaacttata
     5281 ggaagttttt atctatacca atcagtgtag aaagaactac catgtaatga ttctagaaca
     5341 ctgatattaa taaaacaagc aaaaattcat ttttttatgt ttttttaact atctcagcta
     5401 tctatcctct ttataccatt atttttttca aatagtgacc tgtataactt tcttttacgt
     5461 taatgatttc ttctggtgtt ccagtcgcaa ggatcgttcc tccaccttct ccaccttctg
     5521 gtcctaaatc aatgatataa tctgctgttt tgattacatc tagattgtgt tcaatcacta
     5581 aaactgtgtt accagcatct actaatcttt gtaatacatg caacaagcgg gcgatgtcat
     5641 cgctatgaag tcctgtcgtt ggttcatcta gtatatagaa attctttcca ttagagattt
     5701 tgtgcaattc actggcaagt ttcatccgtt gtgcctcacc accggacaat gtcgttgctg
     5761 gttgccccat cgtcacataa cctaagccaa catcaacaat cgtttgcagt ttgcgatgaa
     5821 tctttggaat gtgcttgaag aattctacag catcctctac tgtcatttcc aaaatatcag
     5881 caatgctttt tcctttgtaa tgcacttcta atgtttcaga attatatcgt ttaccatgac
     5941 atacttcaca aggaacatag acatcaggca aaaagtgcat ttcgatctta ataattccat
     6001 ccccgcgaca agcttcacaa cgaccgcctt ttacgttgaa actaaaacgc cctttcttat
     6061 aaccccgcat ttttgcctcg ttcgtttgag caaataaccc acggatatca tcaaatacac
     6121 ttgtataagt cgctggatta cttctcggcg tccggccgat tgggctttga tcgatatcga
     6181 tgatcttttc gatactttcg tagccggaaa ttgtcttgaa tttacctggc ttagcagaat
     6241 tcttatttaa tttttgcgct aacgatttct ttaagatact attgaccaat gtactctttc
     6301 cagaaccaga cacacctgtg actgctacaa attctcctaa tggaaattca acactaatat
     6361 ttttcagatt gttttctgaa gcaccagtga tcttgattgc tttcccatta cctttacggc
     6421 gttcttttgg tacagggatt gattttttcc ctgaaagata tttccctgtc aaagaatttt
     6481 catcattagc tacttcttca ggagtgcctg cagccacgat ttgtccgcct tgttctccgg
     6541 ccccaggtcc gacgtcaatc aaataatctg ctgcccacat cgtatcttca tcatgttcga
     6601 ctacaacgag ggtgttgcca agatcacgca ttttctttaa tgagccaatc agtcgatcgt
     6661 tgtcgcgttg atgcaatccg atcgatggtt catctaaaat atacaaaact ccagaaagat
     6721 tcgatccaat ttgtgtagcc aatctgattc gctgcgcttc tcccccagat aatgttccgg
     6781 aagcgcgact caaagttaga tagtctagtc ccacattttc caagaatgaa agtctgtcat
     6841 ttacttcttt taaaatcggt ttggcaatta ctttctcttg ttcagataac gttaagtttg
     6901 agaagaaatc tactgctttt tgaatagaca actcatttgt ttgcccgata ttcgttccat
     6961 tgatttgaac agatagagct tggggattca atcgataacc tttacatgtc tgacaagtta
     7021 attctgtcat ataaagacgc atttgctcac gcgtgaaatc actgttcgta tcatgataac
     7081 gacgctcgat attcttcaat accccttcga aagtcgtttc tacatcgcga acgccaccaa
     7141 aatcattttc ataatggaaa tggaaaagat catctgtccc attcaagatg atctcctgat
     7201 gctcttccgg taattcttcg aatggtgtat ctaaatcgat accgaaactt tcacaagcct
     7261 gcgctaacat ttgaggataa tactgagagc tgattggatt ccatggaacg atagcacctt
     7321 gactaagtgt cttcgttcta tctggaatca ctaaatcttg atcgacttct aatttgacac
     7381 ctaaaccatc acagtctgga caagctccaa aaggcgcatt gaatgagaac agtcttggtt
     7441 ctagttcgcc aacagtaaat ccgcaataag gacaggcata gtgttcgcta aacaacattt
     7501 cttcttcacc gatcacgtca accagagcat agccgtctgc taatcgcagg gccgcctcta
     7561 aagaatcaaa taggcgagaa cgaatccctt ctttcacaat gatccggtcg atgacgatcg
     7621 cgatatcatg ttttttgttt ttctccagct caggcacttc acttaaatca tagatctcac
     7681 catcgactcg aacgcggacg tatccctctt tttggattcg ttcgaatatt tttttatgtt
     7741 gtcctttttt ctttaccacg atcggtgcca acacttggat ctttgtgcgc tcaggcaaag
     7801 ccattacttg atctgccatt tgatctactg attgactggt aatctcgata tgatcgttgg
     7861 gacaaatcgg atgtcctaca cgtgcaaaca acaagcgtaa atagtcattg atttccgtca
     7921 cagtacctac tgtcgaccga ggatttttac ttgttgtttt ctgatcaata gaaatcgccg
     7981 gactcaatcc gtcgatgcta tctacgtccg gcttatccat ttgtcctaaa aactgacgag
     8041 cataagcaga aaggctctct acgtatctcc gctgcccttc agcgtacagc gtatcaaatg
     8101 ctagggaact ttttccagaa ccggacaaac ctgtaacgac cacgaattta tctcgtggaa
     8161 tcgttacatc tatatttttc aagttatggg cacgtgcacc atgaatcgta attttatcgt
     8221 tagccatgtt gtcctcctac tttgaagctt ttaattctaa aatcatatct cgcacattcg
     8281 cggctgtttc aaaatccaaa gcttttgccg catctttcat ttctttttct aatttcatca
     8341 ataacgtgtc tttttcttcc ttcgtcattt cttcgtacga gacttgtacc gcttcttttg
     8401 tatcatcttc cgattctttc gtaatagaaa tcaaatctcg gatttcttta atgatcgttt
     8461 ttggaacgat cccatgttct tcattgtact tctgctgaat cgtacgacgt cttgacgttt
     8521 catccatcgc tagacgcata gaatctgtta ctttgtctgc gtacatgatt actttacctt
     8581 cttcattccg agcagctcgt ccaatcgttt ggactaagga acgttcacta cgtaaaaagc
     8641 cttctttatc cgcatccaag atagcaacaa gggaaacttc aggtacgtct aatccttcac
     8701 gaagcaaatt gattccaact aacacatcaa attcacctaa tcgaagatct cgaatgattt
     8761 ctgttcgttc cagcgttttg atatcactat gcaaatattt gactttgatc cctaattctt
     8821 tgaaataatc tgtgaggtct tctgccattt ttttcgtcaa tgtcgttaca aacacccgct
     8881 ggtctttttc gactcgttca tttatttcgc ctactagatc atcgatttgt cccatgattg
     8941 gacgaacttc gatcactggg tcaagcagcc ctgtcggacg gatgatttgt tggataacgg
     9001 tatctgtttg ttcatgttca tatggtcctg gcgtcgcaga gacataaatg atctgattga
     9061 catgtttttc aaactcttct aaccgaagtg gccgattgtc taaagcagat ggcaaacgga
     9121 aaccgtaatc tactagcatc tgttttctcg ctcggtcacc attatacatt cctctgattt
     9181 gaggcatggt tacgtgcgat tcgtccgcca cgatcaaaaa atcttcagga aagaaatcca
     9241 atagcgtata aggcggttca ccttctttac gtccgtccat atgccgagaa taattttcaa
     9301 ttcccgacgt gtagcccatt tctaacatca tttcaatatc gtaattggtt cgctgttcta
     9361 aacgttgagc ttccagaagt ttgttttcat ttcgcaagac agttaaacgc tgttctaatt
     9421 cctctttgat attcgctacg gcatgctcca tatgttcatc attcgtcaca aagtgagtcg
     9481 ccgggaagat tgctacgtgt tccgtttctc ctaaaacttc acctgtcagt gcattgactt
     9541 ctcggatacg ttcgacctca tctccaaaaa actccacacg taaggcacgt tcatctcttg
     9601 aagccggaaa aatctccacg acatcaccgc gcactcgaaa acgaccgcgc tgaaaatcta
     9661 tatcatttct ttcgaattgg atactgacta agtcacgaat caactgattg cgatctagtt
     9721 ctgctccctg tcgaatcgaa actacttgtt tttggtactc aaaaggcgag cctaatccaa
     9781 aaatacatga aacggatgca atgacgatca catcatttct ttctaataat gaactagtag
     9841 ccgaatgtcg taatttgtcg atctcatcat ttacacttga atctttttcg atatacgtat
     9901 cgcttgaagg cacataagct tcaggctgat aataatcata ataagaaaca aaatattcaa
     9961 cagcattgtt agggaaaaat tccttgaatt ccccgtacag ctgaccagcc aatgttttgt
    10021 tatgggcaat aatcagtgtt ggtttattca ctttctcaat aagattggaa atcgtatatg
    10081 ttttccctgt tccagtcgca cctaataaga tttgtgcttt tttcccgccg actacgccat
    10141 ccactaattg gttaatcgct tcaggctgat caccagccgg ttggtatttc gagacaagct
    10201 caaagtgtcg ggaggtatct ctctcaatca ttcttcgtcc tccttttcat tcattccttt
    10261 tcattttacg accaataaaa agaaaaggaa agcaaaggca ttccttacta aaaaattata
    10321 ccacaccgaa catactttcg ccactttttt atcttattat acatgttatg ttttataaca
    10381 taaaatgtta gaaactcgca tttttttcat tttttcgagc ttacagtctt gtctgattca
    10441 gcacttttcg cctataataa taaaatattg gaaatttagg aggttgatta tgaaaagaaa
    10501 gcatttattt cactcatttg ttttcttaat gacaggactt gtgacacttg cgtttggtca
    10561 tgttgcacaa gctgacaaca ctacaacggc agagtcaagc acaagcgaga caatgcaaaa
    10621 aatagagccc aaaaaagacg tatacgtaat tgccagtgac tctgcttttg ctccatttga
    10681 atttcaaaac gcaaagggcg agtacgaagg catcgatgta aaattgatgg aaaacatcgc
    10741 caaactgcaa ggatttaaca tccgaatgga ctaccgtggt ttctctgctg ctttgcaagc
    10801 tttggaatca ggacaagcag acggaatgat tgccggaatg actgtaacag atgaacgaaa
    10861 gaaaagctat gatttttctg attcttactt tgatagcggg atccaattag ctgtcaaaaa
    10921 aggagatact tcgatcaagt cttatgaaga tttgaaagga aaaacagtcg gagcaaaagt
    10981 tggtaccgaa agtgctgaat ttttaagcga ccatgaatct gaatatggct attctatcaa
    11041 atattttgat gctgctgatc aattgtacga cgggttaaaa gttggcgcaa tcgatgcaat
    11101 gatggatgat tatccggtaa tcggttatgc gatcaaacaa ggacaagaat tggaaacacc
    11161 gatcaaaaaa gaatctggcg gaaaatatgc atttgccgtt aaaaaaggac aaaatcctga
    11221 gcttcttaat atgttcaatg aaggattgaa agaattaaaa agaacaggcg aatacgataa
    11281 gattattaaa gaatatgtag cggatggttc agaaacttcc agtgagtctt ccactgctga
    11341 tgaatcaaca tttcttggac ttatccaaaa caactatcaa gcattgttga aaggactttg
    11401 gagaacaatt ctcctaactt tgatctcctt tgctttggct atagtaatcg gtgtgatttt
    11461 tggattattc agtgtagcac caatacgcgc tcttcgcaca attgctagta tttatgtcga
    11521 tatcatccga ggaattccaa tgatggttct tgcgttcttt attttctttg ggttgcctgg
    11581 tatcgttgga tttacgattc ctgacttcct tgctggtgtt atcaccttga ctcttaacgc
    11641 aagtgcttat atcgcagaga ttgttcgagg cgggatcaac gccgttcctg tcggacaaat
    11701 ggaagcttcc agaagtttag gtctttctta taatcggaca atgcaaaaaa tcattttgcc
    11761 gcaagcgata cgcatcatga tcccatcatt cgtcaatcag ttcgttatct cattaaaaga
    11821 cacaacgatc atttccgcaa tcggtgtagt cgaattgctg caaactggta agatcattgt
    11881 tgcacgtaca acacaaagta cgtatgttta cttgatcatt gcgatcatgt acttgatttt
    11941 gatcacagca ttgacaaaat tagcaaaagt tttagaaaag aaggtgaagt aagatggcag
    12001 aaaaaatctt agttgagcat ttagtcaaaa aatacggcga taacacagta ttaaacgaca
    12061 tcaatgtatc catcaacgaa ggagatgtcg tctgtatcat tggcccttca ggttcaggaa
    12121 aaagtacatt cttgcgttgc ttgaatcgtt tagaagaacc ttctgacggt gaaattatta
    12181 ttgacggcgc ccacttgatg gataaaagca cagatatcaa cttggtgaga caacatatcg
    12241 gaatggtatt ccaacacttt aacctattcc ctcatctttc tattttggaa aacatcattt
    12301 tagcaccgat ggacctgaag aaagaatcta gagaagaagc agagaaaaaa gcaatcaaat
    12361 tactgcagac agttggtcta gaagataaaa aagatatgta tcctgaaatg ctttctggtg
    12421 gacaaaaaca gcgtgtggct attgctcgtg ctctagcgat gaaccctgac atcatgcttt
    12481 ttgatgagcc tacttctgca ctcgatcctg aaatggtcgg tgatgtcttg aacgtcatga
    12541 aaaagttagc agaacaaggt atgacgatgg ttatcgttac gcacgaaatg gggttcgcta
    12601 aagaagtggc caatcgcgtc atgttcattg acgacggaaa ctttttagaa gatggaacgc
    12661 ctcaacaaat ctttgaaaat ccgcaaaatg aacggacaaa agatttcttg aataaagtat
    12721 taaatattta aaataaatta tatatgtctt tttgactatc tttttatatc ttcttaaatg
    12781 tccgcaaagc cttattttat aggctctacg ggcattttgc ttttgtggta aacctcacat
    12841 atctaggtct atcttcttat attttcacta tcaagcgtgg ttaaaatcgt ggtaaatact
    12901 tctatgcaat tagacgctga acctctgatt ttgcggtatc tatggacgca tgagcatacc
    12961 agttcattgt gatactgata tttgaatgcc ccatgatata ctgtaaatct tttgggttca
    13021 tgttcttgct tgccagtctt gtgcagaatg tatggcgtag cgtatgcggt gtgatatgtg
    13081 gcaatggatt gtccttgtgg tgcttgttgt atttctttac catacggata aataaggcgt
    13141 tataatcaat cgcaactttg ggcttgccct tatgattgac aaataggaag ttgccccgtc
    13201 cgtctatcac aaatggttct gcctttggac gttttttcat aacctgttga aatgcctgta
    13261 tcgtttcttt gcttaatggt acttgcctta ttccgctttt tgttttaggc gtttcaatat
    13321 aatagccctg ttccttgctc tttagtaact ggtggtcgat aatcacaact tcattcttga
    13381 aatcaatgtc ggctactgtc agtccgcaca gttccgaaat acgaagtcct gtctttaaca
    13441 gtatcagcac atcatcataa tgcttgtgat acacattgtc cgtcttgatg aatgagagta
    13501 aggcttgttc ctgttcctct gtcaatgcga ctttcttttt ggtatcattt tctaggactt
    13561 cactcaactt gaaatcaaag ggatttttcc tcacataatc atcttgaatg gctatgtaga
    13621 atgacgcttt taacgagcgt ttatggttgt taatggtatt gtaggaaaag cctttgtctt
    13681 tcatgcgtaa cgcccattcc ttagcgtcag agggtttgat tgtatcaatg ctcctagcac
    13741 ctaacttgtc ctctttcaat aaccgcatga gttgttcccg ttgtttctgt gtgcttttct
    13801 tcacatttgc cctctgtgcg ttctgtttgg cataaagttg gcaaagcgtc attttcttgc
    13861 cagtgctgtc gataccgtcc tcaatatccc gtcttatctg ctgttccagt tctcgaagtg
    13921 agggtttttc ccgttttccc tttggtgtcg ggtctgtggg tgtcaatctc caagcataca
    13981 catattttgt gtttccaaat gcgtctacat atttatataa gtattttctg tctgttcgtt
    14041 ggctctctcc agtatgcagg atacgacctt tgttatcccg tcttttttct ttcatggtgt
    14101 ctgctccttt ccttgttgga aagagccttg atatgacttg tgttcatcat aacacataca
    14161 aggctcattt gcattagata gcgtccaatt tatcaatgac ctgttcaaac tgtcggcgtt
    14221 taatctgtat gcggttgcca ttcataatga gccagccagc gtccttgttt tcctctgcca
    14281 atcgtcttaa cttgttttct ccgatacgga aatactttga cgcttcttca atggtaaggg
    14341 tgtatttttc ccatacggga atatcctgtg cattactcat aaaacacccc ctcactttcg
    14401 attacgtctg ctatgtccgt acacatctgt tccacaagca tttcctgctt ttcagagagg
    14461 tgggtacatt catcaatcat tttcatgtag tttgagcctg tcagcctgtc gatttcctct
    14521 atcatcacga ttgacggcat gacctgtctt tccagccatt gcagagtgcg ttccatattg
    14581 acaggtacgg ggttcatttc aaaaggtaaa ctgtcatggg aaatgaatat ctcccacagc
    14641 ggatagtcgg gaaattgcac aaaatcagta ataaggtaaa acaccagccc atgagggtta
    14701 tatgtcaata acaattcctc aactgcctgt actgcttttg taccacgcag acgaatttca
    14761 aaacgattga taatatccgt atgtttcttt ttggttgcct gttccttttg tttttcatac
    14821 aggcagaagt attttgtgct tgcctttgaa ccgatataaa gggtgcttgc taaattacgg
    14881 ttttttccgc ttagttttcc accctgcaca ttttcataat ttttacagcg acaatccgca
    14941 ccgccatttc tgtatttttc agacaaaacg ggaatatcca acagaccaca catatcattg
    15001 attgccaagt caaaacgtcg gataacaccg tcacagtcaa gacagcggtt taaaaatgaa
    15061 taccaatctc tgttctgtgc ctgcaagaca tattccatgt tgcgtgagcc tgcccctttt
    15121 aattccaaga atacgcccat gtgttcgtca tcagaagcca tgaccttgat ttctccgtag
    15181 gcgtaatgct ctttatagcc ataataccca tagtcataat ggataaaata atctgctttc
    15241 atgccaagca cctttcggat aatttccaat gcgtcagtcg ttggataacg gacagacaga
    15301 taatcaacga gcagataaaa aggcttatcg cagatataat aattcagaca acgcaggata
    15361 atattttgtg tttccttgct ggcgttggct tttccgttct caaagcggtt gatactatat
    15421 cttgatacat gggtaagact tgcaagttct ccttgtgaaa gaccgcttgc aagacgtttg
    15481 ttcttcattt caagtgcaaa ttctaaattg ttcattgtat tcctccaatc ccacaatagc
    15541 cttgaaatac ctgtaaacct ctattcaaaa agtgtgatat gcactatctt cttaaaactt
    15601 ctcattacta ctcacaactt tgggattgta tggtttttga cttgtctttt gtgaaatagt
    15661 gcccccctgt tagataacgg gggcacagac aaggctcgca ggctcgccag ccgacaaagc
    15721 atttcttccc cttgacacaa ggtgtcatgg cggtaagcct gagaggcttc cgccaccgtg
    15781 tcagaaatac tttgtcagca agttctgttt tgccttttct aacaagttcc atttcaaccg
    15841 atattcatat ctacggattt aatcagatag gtatttcttt ttgaatgttt ttatatttca
    15901 ttctattgtg cttattcgat tacaagaaca tagtaattct tatatgctta tttgtcaata
    15961 tctttttttg cctttacaaa tcatattaag catgatagac ttataatagc gaagatataa
    16021 cgagatatgg agggttatgc aatgaaaata agccatttag gaaataatat acagaccata
    16081 agaaaattta ggggaatgaa acaacaggaa cttgcggaca aaatcggtat caatatgcag
    16141 agcctttcca agattgaaag agggttgaac tatcctgcct atgaaacgct ggaaaagata
    16201 atggaagtgc tggacgttac gcccaatgaa ttgttgtcgg gagaatggaa gtatgtcaat
    16261 caatctgaaa aggaagtctg tcagttttta agagccgaag aacgtctaaa tgcagaactg
    16321 aaacatggac actatgataa cttctttgac agcgaggaag aatggctgga atatgagtta
    16381 gaaaagttac gggaatatat tacagactat atcaacggga aaagcattga agcgtccgac
    16441 ctttacccaa tcaaagaatt tatccagcat ttgaagtttc aaaaattatt agaccgctat
    16501 gatgatttat acagtatgga tatgtttggg gaaagcatag agggacataa gtataagaca
    16561 ccttatcaag tcgtgaaaat gataaacccg aactcaaaag aagatatgga actgttacga
    16621 gaagtattaa ggaataacaa ttttgatgat gaggacgagt aacttttttc ctcttgcgag
    16681 aagatacagc aaacaaaaag cccgatcaag agtgcaaagc accaccaatg gtggcaaagc
    16741 tcttgacagg gctttttctt ttctgttgtg ggcaatcaag aggaagaaag ggataaaatc
    16801 tgatttgtgg taaatcaggc aaaggcaacg caggtatctc aatttgattt ggtacttgaa
    16861 aagaacggga gtaattggaa gattatagaa taacaaatat tggtacatta ttacagctat
    16921 tttgtaatca cgtactctct ttgataaaaa attggagatt cctttacaaa tatgctctta
    16981 cgtgctatta tttaagtatc tatttaaaag gagttaataa atatgcggca aggtattctt
    17041 aaataaactg tcaatttgat agtgggaaca aataattgga tgtccttttt taggagggct
    17101 tagttttttg tacccagttt aagaatacct ttatcatgtg attctaaagt atccggagaa
    17161 tatctgtatg ctttgtatgc ctatggttat gcataaaaat cccagtgata aaagtattta
    17221 tcactgggat ttttatgccc ttttgggttt ttgaatggag gaaaatcaca tgaaaattat
    17281 taatattgga gttttagctc atgttgatgc aggaaaaact accttaacag aaagcttatt
    17341 atataacagt ggagcgatta cagaattagg aagcgtggac aaaggtacaa cgaggacgga
    17401 taatacgctt ttagaacgtc agagaggaat tacaattcag acaggaataa cctcttttca
    17461 gtgggaaaat acgaaggtga acatcataga cacgccagga catatggatt tcttagcaga
    17521 agtatatcgt tcattatcag ttttagatgg ggcaattcta ctgatttctg caaaagatgg
    17581 cgtacaagca caaactcgta tattatttca tgcacttagg aaaatgggga ttcccacaat
    17641 cttttttatc aataagattg accaaaatgg aattgattta tcaacggttt atcaggatat
    17701 taaagagaaa ctttctgccg aaattgtaat caaacagaag gtagaactgt atcctaatat
    17761 gtgtgtgacg aactttaccg aatctgaaca atgggatacg gtaatagagg gaaacgatga
    17821 ccttttagag aaatatatgt ccggtaaatc attagaagca ttggaactcg aacaagagga
    17881 aagcataaga tttcagaatt gttctctgtt ccctctttat catggaagtg caaaaagtaa
    17941 tatagggatt gataacctta tagaagttat tactaataaa ttttattcat caacacatcg
    18001 aggtccgtct gaactttgcg gaaatgtttt caaaattgaa tatacaaaaa aaagacaacg
    18061 tcttgcatat atacgccttt atagtggagt actacattta cgagattcgg ttagagtatc
    18121 agaaaaagaa aaaataaaag ttacagaaat gtatacttca ataaatggtg aattatgtaa
    18181 gattgataga gcttattctg gagaaattgt tattttgcaa aatgagtttt tgaagttaaa
    18241 tagtgttctt ggagatacaa aactattgcc acagagaaaa aagattgaaa atccgcaccc
    18301 tctactacaa acaactgttg aaccgagtaa acctgaacag agagaaatgt tgcttgatgc
    18361 ccttttggaa atctcagata gtgatccgct tctacgatat tacgtggatt ctacgacaca
    18421 tgaaattata ctttctttct tagggaaagt acaaatggaa gtgattagtg cactgttgca
    18481 agaaaagtat catgtggaga tagaactaaa agagcctaca gtcatttata tggagagacc
    18541 gttaaaaaat gcagaatata ccattcacat cgaagtgccg ccaaatcctt tctgggcttc
    18601 cattggttta tctgtatcac cgcttccgtt gggaagtgga atgcagtatg agagctcggt
    18661 ttctcttgga tacttaaatc aatcgtttca aaatgcagtt atggagggga tacgctatgg
    18721 ctgtgaacaa ggattgtatg gttggaatgt gacggactgt aaaatctgtt ttaagtatgg
    18781 cttatactat agccctgtta gtaccccagc agattttcgg atgcttgctc ctattgtatt
    18841 ggaacaagtc ttaaaaaaag ctggaacaga attgttagag ccatatctta gttttaaaat
    18901 ttatgcgcca caggaatatc tttcacgagc atacaacgat gctcctaaat attgtgcgaa
    18961 catcgtagac actcaattga aaaataatga ggtcattctt agtggagaaa tccctgctcg
    19021 gtgtattcaa gaatatcgta gtgatttaac tttctttaca aatggacgta gtgtttgttt
    19081 aacagagtta aaagggtacc atgttactac cggtgaacct gtttgccagc cccgtcgtcc
    19141 aaatagtcgg atagataaag tacgatatat gttcaataaa ataacttagt gtattttatg
    19201 ttgttatata aatatggttt cttgttaaat aagatgaaat attttttaat aaagatttga
    19261 attaaagtgt aaaggaggag atagttatta taaactacaa gtggatattg tgtcctgtat
    19321 gtggaaataa aacacgatta aagataaggg aagatactga attaaaaaaa ttccccctct
    19381 attgtccgaa atgcagacaa gaaaatttaa ttgaaataaa gcagttcaaa gtaactgtga
    19441 ttacagagcc agacgcaaag acgcagagcc gataaaatga gattaataca atctcatttt
    19501 atcggctctt tccgttatgt atggattctt ttaattagtc ttcgatgttt cttgcttcgt
    19561 tgataccgct ggctaaagat tccattaagg atagttcttt gtctgtaaag ctatccatgt
    19621 atttctctat ctgtaatcgt cgggtgcttt ttaccaagtt attagcaggt aagaaaaatt
    19681 catcaacgga aacatgaagt aacgatacaa ggtcataaag aacttgtatg ctggggtgtt
    19741 gccctttatt ttcaatatta gttaagtacc gtgggtcaat ttcaatcaat gctcccactt
    19801 gttcacgagt taaacctcgt ttcaatcgag cttctttaat ggctaaacca aaggctctaa
    19861 aatcatattt atcttctttt ttacgcatag tagaccacct ctatacattt tattgttcct
    19921 actgaattaa aaacaggtat agaaaaacgt gttatatggt ttataggttt atatttaata
    19981 aaaagcacta ctaaacgcca ataaaaaaaa ccgttatatg gtagtgctat ttacgctgtt
    20041 aaaatattgt atattacttc caaatggcgg tttgttggag gtcaacgtcg ccatgaagta
    20101 catcatatac aataaatttc cttacattgg gttcttgtca aaaaaagtcg tctatctgca
    20161 atagataagt acgtccacca atgtggtttt ataaatcata tagatagaat aacagaagca
    20221 tgtaaacaga gaaataaatc tgtttatatg cttttttggc tattcagaac ttttttacaa
    20281 agtttattta tcagtaatgc aacaaatccc cctttcacat tgggactaag agtgaaagga
    20341 gataaacgag caaggctcac ttcctttcct agacagaaag ggggtgagat tatgaggaca
    20401 tcttctttcg agcatatcgt cagattacag ttcaattctt taatgctgat tgtcattaag
    20461 aacaaagtga aaagtcgaaa cagacagctt gcaagacatt ctaaacgtga agtattgttt
    20521 tgtgaaatag ccgaaacgaa acaagttgaa cgtggaacga ttgatacctg tttttatgat
    20581 tgtgtatcat ttaaagtctt gcactttaca atttatgtat ctgatgaaac acttggtaca
    20641 gttttacata gactatcaga aaaacagcgt agtgcgattt tattacgtta ttttcaagga
    20701 atgaacgaca gagaaatttc agaactgtac catgtatcac gttctgctat ttgcagtaga
    20761 agaactagag gactaaggaa gttaaaaatg cttttgagcg aaaggaagta aagattatgg
    20821 ctatccctct tttgaattga tttgcaaagc gtcaagcgga gatgaaatgg cagttaaaga
    20881 gattttgaag ttttatgacg gttatatttc taaattatgc ttgcgtcctt tttaccactc
    20941 tgaaagtggt aaaatcatta tgcaggttga tgaagaacta aaaggagaaa ttcatacaga
    21001 catgatgaaa gcaatattga aatttgaaat cggagtgaag taatcaactg tataaaaaaa
    21061 tcgggagata cacagtttcg tgttatctcc cttagtagtt ttacggtcat atcaaggctc
    21121 attcaatcgt ggtaaattcg tggtaagatg agtgtttttc tatacttcaa aatgcttgaa
    21181 tgtatagtgt ttatgcggat aattgaaaat tagatataaa atttaataaa aaccaaaaat
    21241 gcacggtcgc catgaaggga ccgtgtattt ttatcttctt attttctcac ctaatcgtat
    21301 cagccgcatc gcttgttttc ctgcacaaac aagcaatcgc tctacctgtt ccatcgcata
    21361 agaaagtacc atcggttcat tgatgatatc aagtacatag tcgataccac cctcacttaa
    21421 ttgagcttga tttttcttct ccatattatg tagttgtggg acaactttct gacaatcagc
    21481 aattgtgtca agaacggatc aatcctttat tggagttgct cgaaagggtc ttctcttatt
    21541 acggacaggc tttgctcact gtacatagac aacaaatcat tatcctagtc aatcggattt
    21601 ccagagcctc attgctatca ttgcttgata aaatccagac aaagtttaat aaaatgtatc
    21661 agttacagct aaactttgga attgggtcgc tttgttatac agagcaagag acaccgcaat
    21721 ctttccttca tgccaaacaa gtttgcgaat ggatagcttt ccatcaatct gtgaacgaga
    21781 tccgcttttt tgaagattta gacctcggga tcgttttacc agcaatcccg tcagatcaac
    21841 gaacattata tgtaaaacgt attttgaaaa gcctgacaga agaagaagta catttgttca
    21901 agaagacttt ggcatgcttc agtaaaaata acggaagcat caaaaattgc tcagaagaat
    21961 tatttattca taaaaacacc ttgcaatatc gattgaacaa gttccattct ctcactggtt
    22021 atacccctag aaactatgac gattatcata ttctaaagct ggcttttttg ttggtacaaa
    22081 catgacaact acaaaaaaac ggctctataa tttatgggac tgatgaatca gaa
//
LOCUS       DAZKLM010000269        16838 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3764
            SAMEA115706913-rid22582563.guided.060, whole genome shotgun
            sequence.
ACCESSION   DAZKLM010000269 DAZKLM010000000
VERSION     DAZKLM010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706913
            Sequence Read Archive: ERR13196647
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16838)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16838)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287823.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287823.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,004
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,849
            CDSs (with protein)               :: 2,849
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 41 of 96
            Pseudo Genes (incomplete)         :: 52 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 15 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16838
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706913-rid22582563.guided.060"
                     /isolate="VRE3764"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(134..577)
                     /locus_tag="ACG842_002946"
     CDS             complement(134..577)
                     /locus_tag="ACG842_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HFQ0249368.1"
                     /translation="MKKYWKFLLMIGVSTVVMFGMMYLNVYALDHIFFSRTRVFMALM
                     MGAVMAVIMLLFMWKMYENKKINVMILAASLLVFAGSLFMVRSQTTVNDTAWMKAMIP
                     HHSIAILASKRADLKDPEVKKLAEEIIQAQEEEIDKMKKMIKEME"
     gene            complement(795..2135)
                     /locus_tag="ACG842_002947"
     CDS             complement(795..2135)
                     /locus_tag="ACG842_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFQ0249369.1"
                     /translation="MEDKKLKKQLSSRHITMLALGGAIGAGLFKGSGEAIGIAGPSVL
                     IAFLIGGLILYIVMKGLGKIVLSGGDTHHGLSGLIRPYLGAHSADFTDWVYWSMWIIN
                     IIAEAVAAASFLQLWFPHIPAWVFVFMLAVLTTIINLYSVRLFAETEYWLAFAKISVI
                     ILLIIFATYLVGQQMLGTGVFPTLQQLTDHGGFTPHGMKGIISSLLVVIYSYGGSELI
                     AITVSEADDPKRAIPKAIKGVMGRIISFYIIPLFLLLILFPWNTLAGTNVSPFVMVFE
                     KMNIPFAADIVNFVIVLALFSSINSGVYASSRILFFRLKDRKGSSRKLAVLNKHQVPQ
                     RAVLFCTSVLYLGVALSYFVGDKLFGYLAGSLSYTVLLIWIIISAASFVLALRKGTLW
                     DKGISFFALAVLGLIFLGILFTNSIGVTVLTGLLYLFIYFSYQKKNDAFVLTNE"
     gene            2404..3300
                     /locus_tag="ACG842_002948"
     CDS             2404..3300
                     /locus_tag="ACG842_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HFQ0249370.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMNFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPILSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
     gene            complement(3450..4391)
                     /locus_tag="ACG842_002949"
     CDS             complement(3450..4391)
                     /locus_tag="ACG842_002949"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HFQ0249371.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            4517..5638
                     /locus_tag="ACG842_002950"
     CDS             4517..5638
                     /locus_tag="ACG842_002950"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HFQ0249372.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            5896..7398
                     /gene="eat(A)"
                     /locus_tag="ACG842_002951"
     CDS             5896..7398
                     /gene="eat(A)"
                     /locus_tag="ACG842_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection-like protein
                     Eat(A)"
                     /protein_id="HFQ0249373.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            7465..7992
                     /locus_tag="ACG842_002952"
     CDS             7465..7992
                     /locus_tag="ACG842_002952"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HFQ0249374.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQKLAEKVVDL
                     PVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYDGLPSKDENFQKAR
                     LAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIRVINAYFHPMPNEG
                     FFTFMVDNTELVSYE"
     gene            8203..9264
                     /locus_tag="ACG842_002953"
     CDS             8203..9264
                     /locus_tag="ACG842_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MupG family TIM beta-alpha barrel fold protein"
                     /protein_id="HFQ0249375.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            9267..10163
                     /gene="murQ"
                     /locus_tag="ACG842_002954"
     CDS             9267..10163
                     /gene="murQ"
                     /locus_tag="ACG842_002954"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA];
                     GO_process: GO:0046348 - amino sugar catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HFQ0249376.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            10177..12159
                     /locus_tag="ACG842_002955"
     CDS             10177..12159
                     /locus_tag="ACG842_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose PTS transporter subunit IIA"
                     /protein_id="HFQ0249377.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSTATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKVVPQTSA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVVSSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAAGK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(12224..12670)
                     /gene="nrdI"
                     /locus_tag="ACG842_002956"
     CDS             complement(12224..12670)
                     /gene="nrdI"
                     /locus_tag="ACG842_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HFQ0249378.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEHEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQTFGFPFLADYELRGTPSDVTRIYQILAENNK"
     gene            12881..14305
                     /locus_tag="ACG842_002957"
     CDS             12881..14305
                     /locus_tag="ACG842_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="basic amino acid/polyamine antiporter"
                     /protein_id="HFQ0249379.1"
                     /translation="MDTEQQKKGIGLIPLAALVIGSAIGGGVFGIMTDISGSAGGPAI
                     FSWVLVGTAMLMLSLSINNINKKRPDLKGGIFSYAEEGFGRFAGFISGWGYWLTCWLG
                     NVAFATLLMSALGYFFPVFEGGQNLASIVMSTVFLWGYAWLVNRGVENASFVNAIVTF
                     CKLVPLFLFIVVAISTFSIQQFIDNFSSDIILNANGQPIPFSSQVLSCIMVMLWVFVG
                     IEGASVVGSRAKKQSDVGKATILGIFGLIIIYVLVSMLPYGTMTMNQLQTIKAQPAMG
                     YVFEMMVGKWGAVVINGGLIISLVGVWLSWTILPIETMGNMAEDGLLPKAWGKLNKNG
                     APTYAIVLTTLCTNVFLLSLLFTDSAYNFAYTLGTAAIFFTWLFLGLYQMKLSYQRKE
                     WVQFLVGLLASAFQVWAMLVVAFNEVMLVLVLFLPGIYFYLKARKEQNEPVQHIHLDK
                     ILLIGVTMVGVIAMVLFATGMLHV"
     gene            complement(14486..15259)
                     /locus_tag="ACG842_002958"
     CDS             complement(14486..15259)
                     /locus_tag="ACG842_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFQ0249380.1"
                     /translation="MTLLVKQELFKLIKKKSTAVLSVLLVVLLIGTALLAKKYTTIID
                     PVEMTAQLFSATSWIVFIMIAAASTIISMEAQYGTLKNLLYRKYSRGEILVSKWITLV
                     IYSVYLYLLAIIVTVLMKLILFPSISFTEKVSTGQTLIQSLFTYTLGSYIGLWLILSL
                     VLMIACFINSSGASISAGIVFYFASSIISGILIALIQKWEWIKWNPISMLNLQNQIGN
                     EEIMKQLTHLSTNQMLFGNLAYIVLFLALGYLVFKRKNV"
     gene            complement(15256..16161)
                     /locus_tag="ACG842_002959"
     CDS             complement(15256..16161)
                     /locus_tag="ACG842_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0249381.1"
                     /translation="MKSLSVQELSKNFGHKQALSDVSFSLSPGKIVGLVGPNGAGKTT
                     IMKAILGLIHYSDGTICIGENEISPSSHKGLEQVGALIEYPGIYPFLTGYDHLKLFSE
                     TNDVSAIDTIVNQLKMEKYIHKKAKSYSLGMKQKLGIALALLNHPSIVILDEPMNGLD
                     PQATRDVRKIITDLATQGTSFLISSHLLSELEKMVDELILINQGKIIKQCTMAELNQT
                     AQDFIVISTTNDLAARTILTEAGYTLEDPDKIKLLKENEELLGTVIQLLSAQNIHVTD
                     VQHLTNDLESSLLEILDSVKGEEEK"
     gene            complement(16315..16635)
                     /locus_tag="ACG842_002960"
     CDS             complement(16315..16635)
                     /locus_tag="ACG842_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HFQ0249382.1"
                     /translation="MAWMELIIAGILEVFWSTMMKWSDGFSKINYSFYTVIGMIASFY
                     FLSKAIKSLPMSLSYPIWTGIGAVGSVIIGVVLFHDKLNLSTWFFVGMLLISIIGIKI
                     TSGH"
BASE COUNT         5423 a         3083 c         3463 g         4869 t
ORIGIN      
        1 ctgttcttat tacttgatgc agggcagctt tttcacaacc ccttattcac ggcgttccag
       61 aagtagcttc ttcttatttc ttgaagctga taacttctgg aacatcctct ttttttattt
      121 ggacggataa tggtcattcc atttctttga tcattttttt cattttgtcg atctcttctt
      181 cttgtgcctg aatgatttct tccgctaatt tttttacttc aggatctttt aaatcagctc
      241 gtttacttgc caggatagcg atagaatgat gaggaatcat ggccttcatc caagctgtgt
      301 cgttgacagt agtttggctt cgtaccataa acaaggaacc ggcaaaaaca agaagactag
      361 cggctaagat cataacattt atctttttgt tttcatacat tttccacatg aacaacagca
      421 tgatgactgc catgactgca cccatcatca acgccatgaa tactcgtgta cgactgaaga
      481 agatatgatc gagcgcatag acattcaagt acatcatacc aaacataact accgttgaaa
      541 caccgatcat caacaaaaac ttccaatact ttttcattta aaacaaactc ctttttttca
      601 ttcctttctt ctatcatgtc atgagcatgg agaacaatca attattttgc acagagggtt
      661 tttgaacacc gtttaccaag aggtcatgag actgtttatt cggaaaaagg agttgcaaca
      721 agacttttgt cacaactcct ttcttcaata gctccatcta ttccacataa ataaaataaa
      781 attttctatc gatttcattc attcgtcaat acgaacgcat cattcttttt ttgataactg
      841 aaataaatga acaggtaaag caggccagtc aatacagtta ccccaatgga gttcgtgaac
      901 aggataccaa ggaaaatcaa tcctaagaca gctaatgcaa aaaagctgat tcctttatcc
      961 cataatgttc cttttcttag tgccagtaca aaggatgccg cactgataat gatccagatc
     1021 aataatactg tatacgaaag ggaacctgcc agatagccaa ataatttgtc accgacaaaa
     1081 taagaaagag ctacaccaag ataaagaaca ctggtacaaa ataaaacagc acgttgcggt
     1141 acttgatgct tgtttaaaac agctagtttt ctagaggaac cttttcgatc cttcaaacgg
     1201 aagaacaaaa ttcttgaaga tgcatacaca cccgaattga tagatgagaa caaagcaagg
     1261 acaatcacaa aattgacgat atccgctgca aatgggatat tcattttttc aaataccatc
     1321 acaaaaggac tgacattcgt gcctgctaaa gtattccaag gaaaaagaat caaaagcaaa
     1381 aacaaaggaa tgatgtaaaa ggaaatgatt cttcccatga cacctttgat tgctttagga
     1441 atcgcacgtt taggatcatc tgcttcgctt accgtgatag caatcagttc agaaccgccg
     1501 taagaataga taactacaag caaagaactg atgattccct tcataccatg aggagtaaac
     1561 ccaccatggt cagtcaattg ctgaagggta gggaaaacgc ctgtgcccaa catttgctga
     1621 ccgacaagat aagtagcaaa aatgatcagt aagataatga cactgatttt agcaaaagcc
     1681 agccaatatt ctgtttctgc gaataaccgt accgaataaa gattgatgat cgtagttaaa
     1741 acagccaaca taaaaacaaa tacccaggct ggaatgtgcg ggaaccacaa ctgcaaaaag
     1801 gaagcagctg caacagcttc agcgatgata ttgataatcc acatagacca gtagacccaa
     1861 tcagtaaaat cagcagaatg agcacctaga taagggcgta tcaaaccaga taatccgtga
     1921 tgcgtgtcgc caccgcttaa aacgattttt cccagacctt tcatcacgat atataaaatc
     1981 agcccaccaa tcaaaaaagc aatcaataca gaagggccgg cgatcccaat cgcttcgcca
     2041 cttcctttaa atagacctgc accgattgct ccacctaaag ctaacatggt gatatgtctg
     2101 gagctaagtt gttttttcaa ttttttatct tccataaatg acctctttct tttaaattca
     2161 caatcaaaat ttatattaag aataaatagc aatatttaac aatgactaat gataacaaat
     2221 tttcagacaa ttacaactat tcagaaaact tttaggcgaa ttttaataga agaatgattt
     2281 atttcatcat attttcatac aaatgttaaa ttttcataca aatataggaa acgttttact
     2341 ggctttttac tatttaaaca ggctatcata ggaaaaagtg acagagaaag tgaggcggaa
     2401 aagatgaaca aaaatgacgt actgctagtt gcaaaaaata aatcacttta ttttttgcct
     2461 tccatccgct ctttagatgt ttggtccgga aaagatttac tacagtcgga attcattaaa
     2521 tcagctagac aacagttggc aaattatcag ctgatcgttt ttttagatta tggatttgat
     2581 atggagatgg ctcgacgcgt gcgcccattt accaatgcaa aaattgtgtt atttttttgg
     2641 aatcacttca aagcagaaca cgaggcgatg ctgaagcagg cacaagatga accggcaatc
     2701 gatgagattt atcattttga ttttttggaa gcaaaagaac ttgggctgaa acataatagt
     2761 tcattttatt cgaaacatat gaatttttct tctagcgaac caaagacgga tctatttttt
     2821 ggtgcaacga ataatgggag aaaagaacga gcagaaagct ataagcagga atttataaaa
     2881 agagatgtta cgaccaacta tttcattctt ccacatcgag ggaatgaaca acctggatat
     2941 cttacttatg aagagtacct gaaaaaaaca ggggaatcgc gcgggattct ggaactgttg
     3001 cgggagggtc agcaaggagt gactttgcgg acatttgaat ccatgtattt tcaaaagaaa
     3061 ctagtgacgg ataatcaagc gattgctcat tatcattttt atcgtcctga aaatattttt
     3121 ttgcttcgag aacgtgattt agatgaatta ccggaatttc tgcatacacc atttaagcca
     3181 atcctatcag agctattgga attttatgat gcagaaaatt gggcaaaacg atttatcact
     3241 aacgattcca ctatttatga gcaatacgaa tatgacctct cctctgttga aaacaaataa
     3301 aaaacctaca ttttgaagag attatgtact gaaacaaaca agcacaattc ttatggcatc
     3361 atgagagttg tgttttcttt ttgatctttt attgggctta aaaaaagaga accagctgct
     3421 gattcagctg attctcatca gagagccttt tattctgcgc caaggattcc ttcttttgca
     3481 tggactaatt gggtcaaaaa ggcacaatat ggtgtagcaa tatcatattt ttgtcctttt
     3541 cgccaaatgg caccattgat gtagtcgatt tcagtcaaac gatggttttt gatcaggtct
     3601 tggtacatag atgggtaatg caacccgatg ttgtcaggat cataagtaga agcaatatgt
     3661 tggtacactt cttcttgatc tagtatgatt ccttcttttg ctgcgataga agcaaattca
     3721 gatacgattg ttcgaaccat gctctctgcc gctttttgcg cgcctagttc agcaatatta
     3781 caatccaaaa tcgtgcataa accattcaat gttccattta cacaagcttt tcgccaaatg
     3841 gaataacgaa cgttatggct atagatcgga tttaaatttg cttcttgaaa gacttctaca
     3901 actttttttg taaatgcttc gccttctggt tccaagttct ctagttcgat ttctccatca
     3961 cctaacaatg tgacttttcc gggaccagct agaccagctg tccacatcgt gatcccaaat
     4021 aagatatttt tctttggcac atatttttcc agtacatctt catgacctaa tccatttagt
     4081 aaacacaaaa cataagtatt gtcagtaata atggattgga tcgaacaaaa catatcatct
     4141 aattgattgg cttttgttaa tgctacgatc aagtccacgt gttcgttgct ttcgatgatt
     4201 tcttctggtg aatagattgg cagttttgcg accacttctt taccgttaaa gtcagcaatc
     4261 aacccatttg tacgaatggc ttcaatgtga gcaggccaac gatcgatcaa gaggacctca
     4321 tttccgcttt gatgtagcat caatccgatt cggctgccca ttgctccggc cccagctatt
     4381 gcgattttca tgttttttct ctcctttatt caatgagttc ccttttagta tatagaaaaa
     4441 tggaagaaaa tagcattgtt ttgtttgaaa aaaatgaaaa aaagtgtctt acttaagaag
     4501 aaagtgaggg atacagatgg atattttggc ggcaattgat tcatttaaag gaagtgcttc
     4561 ttcatttgaa ctaaatcagg cagttctaaa tggctgggaa gatcgtaaag gaagaaaaat
     4621 caatgtgccg attgcagatg gcggagaaaa tactgcagaa gcaatctatt acgcgctcag
     4681 gggaacatgg cgtaccattg atgggatgga tctcttattc cgtcagcgaa aaatcagcta
     4741 tttattgaca acttggcaag gaaaaaacac cgcagtcatc gaatctgcac aagcattggg
     4801 gttagatcta cttgataaac caacggatca aactatccga cttgcttcca gttatgcctt
     4861 aggagaactt attaaagatg cactgcttca agaagtagaa caaattattg tcactttagg
     4921 aggaagcggg tgtagcgatg gtggtctagg attactccaa tccttgggtg cttctttaga
     4981 agggtacaca gaaggaaatc ctttattatc tactaaaaaa cttcattttg atcgaagaaa
     5041 cgttcttgtt tctaaagtaa aattgattat cgctgcagat gttaccaatc catatacagg
     5101 tcaacaagga gctgcttggc aatttggaag acaaaaagga ggaatagata cgacgctagc
     5161 tttcttagat aaacaagcag gaaagattgc tcagcaaata aaagatttct gggaggtgga
     5221 tttagatgat attccaggaa gtggagcagc aggcggtatt ggtggagccc tttttttgtt
     5281 gggagcgaag atggtttctg gtttcgattt agtcagccaa atcactggat tggaaaaaaa
     5341 gatcgaacaa gcagatgtca tcattacagg agaagggcgg atcgatcagc aaacgatcca
     5401 cggaaaagtt ccatacggtg tagcagtgct tgcaaaaaaa tatcgaaaac caataatcgc
     5461 tatttgcgga agtagagacc aagagctaga aggactttct gattttctcc caattgtctt
     5521 aagtattcat ttgggtccaa taggtttaga agaagcaatg gaacacaggc aaacattaga
     5581 aaaaacaaaa atcttaggac agacacttag cagatttttc accatcctcc cgaagtagtt
     5641 gacaaaatca ttttctttat gctatcgtaa tttcaaatat aggacaaagt gagggatccg
     5701 tgcattgaaa aattaagtcg attgatgaaa gctgcattca gactgtacct tgtggagaca
     5761 aggaagcagt gtgcagattt tcgggattgg cttggttttt tcgatgtatg ggtctttttg
     5821 tgtgtatcca acccttgcat cctctttagc cagttctgaa aataggactg gtcttttttt
     5881 acgaggtgat ggaaaatgtc taaaatcgaa ataaaaaatc tgacattcgg ctacgacagc
     5941 caaggcacat tattatttga acaagcaaat ctaaattttg acacacaatg gaaactagga
     6001 cttatcggac gaaacggtcg aggaaagaca actttactga atattctaca aaacaaacta
     6061 ccttatcaag ggcaagtaat ccatcagcaa gaatttgcct atttcccgca acagacaaaa
     6121 gataaagaac gtttaaccta ttacgtgtta aatgatatta cggattttga gatatgggaa
     6181 atcgaaagag agctccaatt gatgcaaaca gatcctgaaa tcttatggag agaattcagc
     6241 acactatcgg ggggagagaa gacaaaagtc ctactggcac ttttatttgt ggatgacact
     6301 catttcccgt taatcgatga accaacgaat catttggata tctctggtag aaaacaagta
     6361 gcggcttatt tgaaaaagaa aaaacaaggc ttcatcgtgg tcagccatga ccggggattt
     6421 atcgatgaag tagtggacca tgttttagca atcgaaaaaa gtcaactgga actttatcaa
     6481 gggaatttct ctatctatga agaacagaaa aaacttcgtg atgaatttga aatggctcaa
     6541 aatgaaaaat tgaaaaaaga agtcagtagg ctaaagaaaa cagcagctga aaaagccgaa
     6601 tggtctcgtt cccgagaagg agataaaaca aagaaacaag tcggattcat cgatactgaa
     6661 tctagacgag tgaataaagg agcagtgggt gctgatgctg cacggacgat gaaacgatcc
     6721 aaagcaatcg tgaatcggat ggagacccag atcagcgaga aagaaaaact attaaaagat
     6781 atcgaatata tcgattcttt gacgatgaat agccaagcgt ctcaccataa gcgactttta
     6841 agcgtagaag atcttcaatt agggtatgaa aatctgttat tcgagccaat tcattttaca
     6901 atcgagcctc atcagcgggt ggcgatttca ggtcctaacg gtgcaggaaa gtcatccatt
     6961 atccattatc ttctgggggc attcaacggc aaggttatag gagaaaaata ccagccaaaa
     7021 catctgagca ttagttatgc aagccaaaat tatgaagaca atcgaggaac gttggcggaa
     7081 tttgcagaga aaaaccaagt agactaccaa gcatttttga acaacctccg aaagcttggg
     7141 atggaaagag atgtttttca taacaagatc gagcagatga gtatgggcca acggaaaaaa
     7201 gtggaattgg ctaaatcttt atcacagcca gctgaactat atacatggga tgaaccattg
     7261 aattatttgg atgtcttcaa tcaagaacaa ttagaacaac tgatcttgaa cgtgaaacct
     7321 gccatgttac tagtggaaca tgatcaaacc ttcctggata aagtatctac tgagattatt
     7381 tctcttgaga gaatctaacc cttgtaatct caagaacaac aaggtatggt taaggggaac
     7441 tttgccaacg aaggagagaa tcacatgctt tatgtaacga gacacggaga aacaacttgg
     7501 aacgcacaag gattagtttg cggacgggca gatgtgccgc tgactgaaaa aggacagatg
     7561 caggcacaga aactagcaga aaaggtagta gatttgcctg ttccaatcac gaaaatcatc
     7621 cattcccctc ttcaacgagc aagagataca gcacaagcag ttgctgatcg tctcagcttg
     7681 ccgctgaccg ttgatgaacg attggtagaa atggattttg gggattatga tggactgcca
     7741 agcaaagatg agaactttca aaaagcacgt ttagcgttcg ctgtacgatt cccaaatggg
     7801 gagtcagtct tagatgtgta tgcacggatc gttcctttat tgaaagaatg catcgaagac
     7861 gaagaaaatg tctatctgtt agtttgccat aatgccttga taagagtgat caatgcttat
     7921 tttcatccga tgcctaatga ggggttcttt acattcatgg tagataatac agaattggtt
     7981 tcttatgagt aattatgttt ttgtgttatt taaataaaat atttcaaggc actgcttttg
     8041 cttagcagtg ccttttttag gttatgacaa ttcttttgat aatataaact caacctttag
     8101 aaatgaaatt taattatttt aataaaagta ttgaaatttt gtttctttag atatatactc
     8161 tagtcataga aatgaagacg gatacagaaa aagagggata atatgtttgg cttttcaata
     8221 tttatgaacg attctttgtc agaagagaag aaacagtata tccgccaaat ggcatccaat
     8281 ggctttgtcg gtattttcac ttccatgcat ataccagaag atgatgtttc ggcatatcaa
     8341 aagcgattga tcgaactagg aagcgaagcg aaaaagaata aactagaatt gatggtcgat
     8401 atttcaggag aggcattgga acgagcgggt ttttcattag aagatctaac gcctttgaaa
     8461 gcaattggtg taacaggttt aagaatggat tatcatattt ccaatcagca gattgccgac
     8521 tggtcacatc aattgaaaat cagtttgaat gctagcacga tcacgcctaa agatatcgat
     8581 gaattgaaag aagcagaagc tgatttctca cagattgaag cttggcataa ttattatcca
     8641 cgtcccgaaa cagggctgga caaagaatgg tatcagaaaa aaaatcaatg gctcaaaatt
     8701 caaggtctcc ttgtccaagg ttttgtacca ggagatacag agttgagagg cccactatat
     8761 caaggattgc caactttgga agaacatcga gggatccatc ctcttgcagg cgctctagat
     8821 ctattagcaa gtaacacgga tattgtttac atcggagatg caggtctgtc agaaaacgtt
     8881 caggagcaat ttgcttcttt ccaaaaagaa cagacagtct tgctccatac ggaacctgta
     8941 gatgaagaat tttacgaata tatattaggg aagcatacga atcggcaaga cgatgcaaga
     9001 gatgtcatac gaagcgtaga tgcacgtttt cgagagattc cgcctatccc agcaagaaat
     9061 acagctacgc gtatgaaagg gtctattact ttagataacg aaaagtattt gcgctatatg
     9121 ggtgagatcc aattaaccaa atatgatttg ccagcggatg aaaaagtgaa tgtagtagct
     9181 aaagttatca aagaagagct accattgatc aaccaaatca aagctggtat gaactatcaa
     9241 tttataagaa aagaaggaag ataaaaatga gcaaaatcaa tttagagaac ctaacaacgg
     9301 aacgcagaaa ccaagaaact tttggattag atgagatgag tatttctcaa gccgttcaac
     9361 taatgaacaa agaagatcag aaagtggccg ctgcggttga aaaagaactg acaagtatcg
     9421 aaccagtgat tgctaacacg attgagtcct ttaaaaaagg tggacgtctg atttatatgg
     9481 gagccggaac aagtggaaga ctaggtgtgt tagatgctgc tgaatgcgta ccgacattcg
     9541 gtgtagaacc ggaaatggtc ataggtctaa ttgctggtgg agaagaagca atgactgtag
     9601 ctgtcgaagg ggccgaagat tcttatgagc tagggcaaga agatctaatc aagctgaatt
     9661 tgacaaaaaa tgatatagtt attggtatcg cagctagcgg tcgtacacca tatgtgatcg
     9721 gtggattaga ttacgcaaaa tcgatcggtg ctgtcacagg aacgatttct tgcaataaac
     9781 aagccgaaat cagtaaacac gcggatttcc caatcgaagt cgatgcaggt ccagaatttt
     9841 tgacaggctc tactcgattg aagtcaggaa cagcacaaaa acttatcttg aatatgattt
     9901 ctactatcag tatgattggt ataggtaaag tattcaataa cttgatggtc gatgtcaaac
     9961 caacaaatga aaaactaatc gaacgctcaa aacgaatcat catgcaagcg acagaagctg
    10021 attacgaaac agctgaaaag tactttgaag aagcagatca aaatgttaag ctggctatcg
    10081 tcatgatttt gacaaatagt gataaagagg aagctcaagc aaaattagct gaagcaaacg
    10141 gatttgtaaa agggacctta tgagggggaa aagaccatgg caaaaaacga actgactttg
    10201 gaagaactgg caaaacaaat ttataaaggg gcaggcggca tgggcaatgt agaaagcatt
    10261 gtccactgca tgactcgtgt gcgtatgaag atcatcaatg atgatcttgt aaatgtctct
    10321 gaattaaaag cgattccagg agtgcttggt gtagtagaag atgaacagct tcaagtcatc
    10381 atcggacctg ggaaagtgaa caaagtagca cagcaaatgg ttgatttggc aggcgtgaaa
    10441 ttaggagaaa aactttcttc tgtaagtaca gctacagcaa attcatcagc atctggaaaa
    10501 gaccgtgtaa acgaacgggc acaagaaatg aaagcggcac aaaaatcgaa acaaaaacca
    10561 tctaaaatca aatcggtatt aaaagacatt tctaatatct ttgtgccaat gattcctgca
    10621 tttgtcggta ctgggatcgt ggcagggatt gccgcagttt tagcaaactt agtcacagcc
    10681 ggaacacttg atgcagcaac atggcaacaa tatatcgatg tgatgaacat cttgaaaaac
    10741 gggatgttta gctacctagt tatctataca ggtatcaatg ctgcacaagt ttttggtgca
    10801 acaccaactc ttggtggagt tatcggggcc gttgtgatgc tgacagggat gaatccggaa
    10861 gcaccactga aaaacttatt tactggtgaa gctcttgcag ctggacaagg tgggatccta
    10921 ggtgtcatct tcgctgtttg gctattgtca atcgttgaga aaaaactgca taaagtagta
    10981 cctgatgcgg tagatatcat cgttacacca actctttcat tgattgccat cggtctgatt
    11041 gaaattttct tcatcatgcc tttagcaggt ttcatttctg atggcatggt cggtgggatc
    11101 aactgggtac ttggcgttgg tggagcattt gccggcttcg ttctaggcac attattcttg
    11161 ccgatggtta tgttcggact gcatcaaatt ttgactccaa tccatgtgca aatgattgat
    11221 gaaactggtc gtacgctatt gcttccaatt cttgcgatgg ctggtgcagg acaagtgggt
    11281 gcagcattgg cattatggat tcgctgcaaa aaagataaag aattaacaga aatgatcaaa
    11341 ggagctttac cagtcgggat cctaggaatc ggcgaaccat tgatttacgg agtaacatta
    11401 cctctaggac gcccatttat tactgcttgt atcggtggag gtatcggcgg ggcagtgatc
    11461 ggaatgatcg gaaatgtcgg ggctatcgct attggacctt caggagccgc tttgatccca
    11521 ttgatctctg acggtaaatg gttaggctac atccttggat tattggcagc ttatgctggt
    11581 ggcttcgttg ctacattctt cttcggtatt cctaaagacc gtttagaaaa agaagaatta
    11641 gcgactgaaa cagccacagt aaatgaagtg cctgaaaaag tagtacctca aacttcagct
    11701 gaaagtgtga ctatcctctc atcagttgct gacggaacag ttgtttcttt agataaagca
    11761 agtgatccag tttttgcaca aaagatgatg ggtgaaggct actttgtaga accagaaaac
    11821 ggacatatct attctccagt tactggggta gtcagctctg tcttcccaac gaaacacgct
    11881 attgggatca caacgccaaa tggtttagaa atcttgcttc atatgggaat caacacagtt
    11941 gacttaggcg gtacgccatt tgatgtgaaa gtaacagaag ggcagcaagt gacacctgat
    12001 acgcttgtag cagaagctga cttagaagcg atcaaagcag caggcaaaga aacatccatg
    12061 atcgtcttgg taaccaatat ggatcgtgta aaaaactttg tcttggaaaa aacaggtaaa
    12121 gcaaaagcaa aagcacctgt gatggatgtc aaagcttaat atgatagaaa caatagaaac
    12181 gaaagaagaa actgaaatct atttttcagc ttcttctttt ttattattta ttgttttctg
    12241 ctaaaatctg ataaattcgt gtcacatcag atggtgttcc ccgtaattca taatctgcca
    12301 agaaaggaaa gccaaatgtt tgtgcatatt gtttggctgt tagacaatac tgattattga
    12361 agtttttatt tccgctaccg actacaccaa gacaaagttt atgattgtct tcatgttcca
    12421 gatattcacg catcgtttcg gtcaagattt ctgtatcacc gttatctaaa ccatttccgc
    12481 cgtctaaata agtaggaacg aaagtaaaaa atggttctgt ttctttcgta aaatcactat
    12541 tttcatggat ttcttttaaa gtaacttcag gattgacagg atcttctgca tgcatttttt
    12601 cagcatattc tgctagatgt ttggcaaagg cacgcgtatt tcctgaaatc gatatatata
    12661 aaatgttcat ggatcatcct ccaattcttt ccttttagta tagcatcttt tttaagtttt
    12721 ttggtggtgt aatttggaaa gggactaggc gctttgcttg tataatgaga aaaaagccgg
    12781 tagaattata tttctattaa agtttttatt aattaagtaa aaataaaaaa ggtttgttat
    12841 actttttgat aaaagcggtt ttattttgga ggatgcttac atggatacag agcaacagaa
    12901 aaaaggaatt ggtttaattc cacttgctgc cttagtaatt ggttcagcga ttggaggtgg
    12961 cgtatttggt attatgacgg acatctccgg atcggctggc ggcccagcta ttttcagttg
    13021 ggtactcgta ggaacagcaa tgttgatgct ctccttgtcg atcaataata tcaataaaaa
    13081 acgtccagat ttaaaaggcg ggatctttag ttacgcagaa gaaggttttg gccgtttcgc
    13141 tggttttatc agcggttggg gttactggct cacatgctgg ttaggcaacg tagcttttgc
    13201 aacattgttg atgagcgcat taggatattt cttcccagta tttgaaggtg ggcaaaatct
    13261 ggcatcgatc gtgatgagta cagtcttttt atggggatat gcatggctag tgaatcgggg
    13321 agtcgaaaat gctagtttcg tcaatgctat cgtcacattc tgtaaattag tgccattatt
    13381 tttatttatt gtagtagcta tcagcacgtt ttcgatccaa caattcattg ataacttttc
    13441 tagcgacatt atattgaatg cgaatggaca gccgatcccg ttttccagtc aagtattgag
    13501 ttgcatcatg gtgatgcttt gggtatttgt tggtattgaa ggagcctctg tcgtaggttc
    13561 acgagcaaaa aaacaatcag atgttgggaa agcaacgatt ctagggatat tcggcttgat
    13621 tattatttac gtcttggttt ccatgcttcc atatgggaca atgacgatga atcagctgca
    13681 aacgatcaag gcgcagcctg ctatgggcta tgtgtttgaa atgatggttg gtaaatgggg
    13741 agcagtcgtg atcaacggcg gtttgatcat ttctttagtt ggtgtttggc tctcatggac
    13801 gatcttaccg attgaaacaa tgggaaacat ggctgaagac ggacttttgc ctaaagcatg
    13861 gggaaaactc aacaaaaatg gggcaccaac ttatgctatc gtattgacga cgttatgtac
    13921 gaatgtcttt ttactttcat tattattcac tgacagtgct tataactttg cttacacact
    13981 tggtacagca gctattttct tcacgtggct tttcttagga ttgtaccaaa tgaaactttc
    14041 ttatcagcgg aaagaatggg tccaattttt agtagggctt ttagcaagtg cctttcaagt
    14101 atgggctatg ctagtcgtag cctttaatga agtaatgctt gttcttgttc ttttcttgcc
    14161 aggcatttat ttttacctca aagcacgtaa ggagcaaaac gagcctgtac aacatatcca
    14221 cttagataag attttactta taggtgtgac gatggtcgga gtgattgcaa tggttttatt
    14281 tgccactggg atgcttcacg tatagaagta taaaagaagt ataaagagag gttgtgagcc
    14341 tgtcgtttat tcaaaaacat actaggaaag gagctgtgac acaaatgttg tcacagctcc
    14401 tttttcagaa taaacggtgt ttaagagaca gccaatcggc aataaggctg aacgtctctt
    14461 tcacagcctc ttttttgatg agtcatcata cattttttcg cttaaatacg agatatccta
    14521 atgctaagaa caaaacgatg taagctaaat taccgaatag catttgatta gtagaaagat
    14581 gagtcaattg cttcatgatc tcttcgtttc caatttggtt ttgcagattc aacatactga
    14641 tcggattcca tttgatccac tcccattttt ggatgagtgc aataaggata ccagatatga
    14701 ttgacgaagc aaaataaaag acgattcctg ctgagatgga tgcgccggaa ctattgataa
    14761 agcaagcaat catcaacact aaacttaaaa tcagccataa accgatataa ctacccaaag
    14821 tataagtgaa caagctttgg atcagtgttt gaccagtaga aaccttctcc gtgaaggaaa
    14881 tcgaagggaa aagaatcagt ttcatcaaca cagtgacaat aatagctaaa aggtataaat
    14941 aaacagaata aataactaat gtgatccatt tgcttactaa tatttctcct cgagaatact
    15001 ttcgataaag gagatttttc aatgtaccat attgtgcttc catcgaaatg atcgtgcttg
    15061 cagctgcaat catgataaag acaatccaag atgtggcact aaaaagctga gcagtcattt
    15121 caactggatc aatgatcgtc gtgtattttt tggctagaag tgctgttcct atgagcaaaa
    15181 caactaatag aacagataaa acagcagttg actttttctt gatcaattta aataattctt
    15241 gttttacaag caatgtcatt tttcttcttc cccctttact gaatccagaa tttccaatag
    15301 agaagattcc aaatcattcg tcagatgctg gacgtctgtc acatggatat tttgtgcaga
    15361 caatagctgg atcacggttc ctaataattc ttcgttttct tttaaaagct tgattttatc
    15421 aggatcttcc aaagtatatc cagcttctgt caagatcgtt cgagcagcta gatcatttgt
    15481 tgtagagata acgatgaaat cttgcgctgt ttggttcaat tcagccattg tacattgttt
    15541 gatgatcttt ccttgattga tcaggatcag ctcatcgacc attttttcaa gctcgctgag
    15601 taagtgactg gagatcaaga aagaggtgcc ctgagtagcc aagtcggtaa tgatttttcg
    15661 cacatctctt gttgcttgtg gatccaaacc gttcatcggc tcatctaaaa taacgataga
    15721 cggatggttc agtaaggcaa gagcaattcc caatttttgt ttcattccca acgagtagga
    15781 tttcgctttt ttgtgaatgt acttttccat cttgagctga ttcacgatag tgtcgatagc
    15841 agatacatcg ttggtttcag agaataactt cagatgatca tagcctgtta agaatggata
    15901 gatacccggg tattcgatca atgctcctac ttgttccaac cctttatgac tagagggaga
    15961 aatctcattt tcaccaatac agattgttcc atctgagtaa tggatcagtc ctaaaatagc
    16021 tttcatgatc gttgtttttc cagcgccatt aggacctact aatccaacga ttttgccagg
    16081 ggataagcta aaactgacat cgctgagggc ttgtttgtga ccaaagttct tggataattc
    16141 ttggaccgaa agacttttca aaaatattca ctccttttct atagatagta caagcataca
    16201 cctgatgagt ggagaagtcg ctaggtcatt ggacttattt cttatttttt cataaaaaaa
    16261 ttgaatatta aataaaaagg cttatttttt ctgatcagta cttattccaa gtgtctagtg
    16321 gccgctagta atcttgatgc caatgatact gatgagtagc atccccacaa agaaccaagt
    16381 ggaaagattc agcttatcat gaaacaagac cacaccaatt atgaccgaac caactgcgcc
    16441 aatccctgtc cagataggat aagagagact catcggcaaa cttttgattg cctttgacaa
    16501 aaagtaaaaa ctagcaatca tgccaatcac tgtgtaaaaa gaataattga ttttagaaaa
    16561 accatcactc catttcatca tcgttgacca gaatacttcc aaaattcctg ctatgattaa
    16621 ttccatccat gccatactta cattcctcct taactataaa aaaatacaca aaaagaggaa
    16681 aatcatccct tcaaagacct aatggaatga tcatcctctt tatcctaccc ggtggcagat
    16741 tgtatatctt tttctgtagt atagcacacc tatccagata tcggcaatct catacttttt
    16801 agtaaagtgt accaagcact gacggatata tcgttagc
//
LOCUS       DAZKLM010000270        13213 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3764
            SAMEA115706913-rid22582563.guided.076, whole genome shotgun
            sequence.
ACCESSION   DAZKLM010000270 DAZKLM010000000
VERSION     DAZKLM010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706913
            Sequence Read Archive: ERR13196647
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13213)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13213)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287823.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287823.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,004
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,849
            CDSs (with protein)               :: 2,849
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 41 of 96
            Pseudo Genes (incomplete)         :: 52 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 15 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13213
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706913-rid22582563.guided.076"
                     /isolate="VRE3764"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(204..2162)
                     /gene="nagE"
                     /locus_tag="ACG842_002961"
     CDS             complement(204..2162)
                     /gene="nagE"
                     /locus_tag="ACG842_002961"
                     /EC_number="2.7.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015572 - N-acetylglucosamine
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-specific PTS transporter
                     subunit IIBC"
                     /protein_id="HFQ0249383.1"
                     /translation="MKAYLQRMGRSLQLPVAVLPAAALLVGIGNWWASYSNDIVAHFL
                     QAGGNAVLGQLPLLFAVGLALGMSVDKSGAAALAGLVAFEVPVNVLKTDSVATLLNIK
                     PESVDPSFAQISNVFIGIIAGLIAAALYNRFHEAKLPMALSFFSGKRLVPILAAFVML
                     LISAVLLLVWPPVYSALVSFAKFILSLGWGGAGLYGFFNRLLIPTGLHQAMNSVFWFD
                     VAGINDIGKFLASEGTKGVTGMYQAGFFPIMMFGLPAGAYAIYRNARPENKAKTASLM
                     LAAAFASFFTGVTEPLEFSFMFVAWPLYVLHAIFTGISLAVSAFFHWTAGFAFSAGFV
                     DYFLSLKNPIANQPLMLIVQGLVTAVIYYFGFNFAIKKFHLMTPGREEADLSDDDTAT
                     TNSDNKYAAQASKIYAALGGADNVTSIDNCTTRLRLQVKDTGLIDQNKIKATGVPGMK
                     IIDGKNAQVIVGTEVQFVADEMAKLHGGAATRPAQTNTVVKTETFAGGTIEQDIYAIA
                     NGKLIPITEVSDDVFAEKMMGDGYAVLPENGEIFSPIAGTITNIFPTKHAVGIQTDAG
                     IEVLLHMGINTVDLKGEPFTLYVKEGQKVARGQLIALVDLAAIQSAGKNTDMVVVFTN
                     GDKVQSLEIEPARDVKANDKIGSVSNKA"
     gene            complement(2159..3031)
                     /locus_tag="ACG842_002962"
     CDS             complement(2159..3031)
                     /locus_tag="ACG842_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFQ0249384.1"
                     /translation="MKIKKIFNQNAVLVDDGGQEKVAIGKGIGFDRKRNDLIFDRDVE
                     RIFVMEPEGQIKLQNLLNQIDERFLFAAETIIDHAETVLMEKLNEHVLIALTDHIAFS
                     AENINNGIVIRNKLLREIEVLYSDEFSIAQWAVEYLTKELGIPYTYDEAGYIAIHIHS
                     GRSGRNNNHRSIREVTIVSDIIHLVESELETDIHSEPFSLNYSRLVNHLRLLLQRTHA
                     QQYAVLDTEIVEMVKRKYPESYKISKKIRVLLTKEYQLAITKEELGYLAIHIERLRSA
                     IVQTNVNNHEEEKN"
     gene            complement(3373..4245)
                     /locus_tag="ACG842_002963"
     CDS             complement(3373..4245)
                     /locus_tag="ACG842_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFQ0249385.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKVFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
     gene            4373..4942
                     /locus_tag="ACG842_002964"
     CDS             4373..4942
                     /locus_tag="ACG842_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DapH/DapD/GlmU-related protein"
                     /protein_id="HFQ0249386.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLSVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            5009..5746
                     /locus_tag="ACG842_002965"
     CDS             5009..5746
                     /locus_tag="ACG842_002965"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFQ0249387.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            5977..7245
                     /locus_tag="ACG842_002966"
     CDS             5977..7245
                     /locus_tag="ACG842_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HFQ0249388.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            7732..8196
                     /locus_tag="ACG842_002967"
     CDS             7732..8196
                     /locus_tag="ACG842_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFQ0249389.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIKH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            8180..8809
                     /locus_tag="ACG842_002968"
     CDS             8180..8809
                     /locus_tag="ACG842_002968"
                     /EC_number="1.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016657 - obsolete oxidoreductase
                     activity, acting on NAD(P)H, nitrogenous group as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HFQ0249390.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMKPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITMFE"
     gene            9163..10641
                     /gene="msr(C)"
                     /locus_tag="ACG842_002969"
     CDS             9163..10641
                     /gene="msr(C)"
                     /locus_tag="ACG842_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(C)"
                     /protein_id="HFQ0249391.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRLDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            10688..11134
                     /locus_tag="ACG842_002970"
     CDS             10688..11134
                     /locus_tag="ACG842_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HFQ0249392.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(11333..11848)
                     /locus_tag="ACG842_002971"
     CDS             complement(11333..11848)
                     /locus_tag="ACG842_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFQ0249393.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            11965..13167
                     /locus_tag="ACG842_002972"
     CDS             11965..13167
                     /locus_tag="ACG842_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFQ0249394.1"
                     /translation="MEILENQIDIQEQTNEETKGKSSALVVAATCMIAFMGVGLVDPI
                     LKTIAAQLNATPAETTLLFTSYMLVTGVVMLFTGFLSSRIGLKKTLMTGLFIIVVFAG
                     LGGLSGNIGMLIGLRAGWGVGNALFVSTALATIVSILVGNTEKAIMMYEAALGCGMAV
                     GPLVGGVLGSGSWRYPFFGVAFLMFCGFIALAVLLPETEKPKRKVGLWEGVKALSNHK
                     LRSVGLIALLYNFGFFTLLAYAPFLLIGYSELEVGFVFFGWGVLLAIASIFVAPALER
                     KFTTYRTILAAFVLFIICLLLLGAGTSMPVLVPISIVLAGFFQGIINTLLTTIAMEIP
                     GLQRNVASSSYSFVRFFGGALAPFIAGVIGEHIGESYAFYFAGAIVLISVCCIIYHRN
                     YFATNEEE"
BASE COUNT         4116 a         2424 c         2708 g         3965 t
ORIGIN      
        1 agaggttgta aaaaagctgt tctgcatcaa gtaataagaa gagtcaaata aaaaatagct
       61 cttcatatgt tacgtttggc tggacttatt accgcggagt tggcaggaga acgccgtttt
      121 tattcggaaa aaggagctga gacaagattt ttgtctcagc tcctgtttgt ttatcgataa
      181 aaaagtctgg ggaagacgat ggctcaggct ttgttggaaa cagagccgat tttatcattt
      241 gcttttacat cacgtgcggg ttcgatttct aagctttgga ctttgtcgcc atttgtgaat
      301 acaacgacca tatccgtgtt tttgcctgca gattgaatag cggcaagatc aactaaagca
      361 atcaattgac ctcttgcgac tttttgacct tcttttacat ataaagtgaa tggttcgcct
      421 tttaagtcaa cagtgttgat gcccatatga agcaagactt cgataccagc atctgtttgg
      481 attccgacag catgtttcgt tgggaaaatg tttgtgatcg taccagcgat tggagagaag
      541 atttctccat tttctggtaa tacagcatag ccgtcaccca tcattttttc cgcgaataca
      601 tcatctgaaa cttcagtgat tggaatcagt ttcccatttg cgatagcgta aatatcttgt
      661 tcgatcgttc caccggcaaa tgtttctgtt ttaaccacgg tgtttgtttg tgcaggtcgt
      721 gttgcagcac caccatgtag ttttgccatc tcatctgcaa cgaattgtac ttctgtacct
      781 acgatgactt gcgcattttt tccgtcgatg atcttcatac caggtacgcc agtggctttg
      841 attttatttt gatcgatcag gcctgtatct ttgacttgca aacgaaggcg tgtagtacag
      901 ttatcgatcg aagttacatt gtcggctccg cctaatgcag cgtagatttt gcttgcttgt
      961 gccgcatatt tattgtctga attagtcgtt gcagtatcgt catctgaaag atctgcttcc
     1021 tcgcggccag gagtcatcag atggaatttt ttgatagcaa agttaaagcc aaagtagtaa
     1081 atcactgctg ttactaatcc ttggacgatc aacatcaatg gctgattcgc aatcggattt
     1141 ttcaaagata ggaagtaatc gacaaatccg gcactaaagg caaatcctgc tgtccaatgg
     1201 aagaatgcac tcactgctaa agaaatccca gtgaaaattg catgcaagac atataatggc
     1261 caagcaacga acataaatga gaattcaagt ggttctgtta caccagtaaa gaatgaagca
     1321 aaagctgctg ctaacatcaa ggaagctgtt tttgctttgt tttctggacg tgcatttctg
     1381 taaatagcat atgctcctgc tggaagcccg aacatcatga ttgggaaaaa tccagcttga
     1441 tacattcctg ttacgccttt tgttccttca gaagccaaaa atttaccaat atcattgatt
     1501 cctgctacgt caaaccagaa cacggagttc attgcttgat gcaatcctgt agggatcagt
     1561 aaacggttga agaagccata aagacctgca ccaccccagc ctaaactcaa aatgaattta
     1621 gcaaaagaaa ctaaagcaga gtaaaccgga ggccatacga gcagtagcac tgcactgatc
     1681 agcaacatga caaaggcagc caagattggc actagacgct tgccgctaaa gaaagataaa
     1741 gccatcggta attttgcttc gtggaaacga ttataaagag ctgccgcaat caatccggca
     1801 ataataccaa taaatacgtt actgatctga gcaaaagatg gatcaactga ttctggtttg
     1861 atattcaata aagtagctac tgaatcagtt tttaaaacat ttactggaac ttcaaatgcg
     1921 accaaacctg ccaaggcagc agcacctgat ttgtccactg acatccctag tgctaaccca
     1981 acggcaaaca gcaaaggtaa ttgacctaaa actgcgttac cgccagcttg taaaaaatga
     2041 gcgacaatgt cgttactata agatgcccac cagtttccga tccctactag taaagcagca
     2101 gcaggtaaga ccgcaactgg aagttgaagg gaacgtccca ttcgttgtag atatgctttc
     2161 aatttttttc ctcctcatga ttgttaacat ttgtttgtac gattgcgctt cttaaacgct
     2221 cgatatggat ggccaagtaa cccagttctt ctttcgtgat cgcgagctgg tattctttgg
     2281 tcagcaacac acggattttt ttagaaatct tatagctttc tgggtatttt cgtttgacca
     2341 tttctacgat ttccgtgtct aaaacagcat attgttgcgc atgggttcgt tgtagaagca
     2401 gtcgtaaatg attcaccagt cttgaataat tcagtgaaaa tggttctgaa tggatatctg
     2461 tttccaattc tgattccact aaatggatga tgtcagatac aatcgtcact tcacggatac
     2521 tgcgatggtt attgttccga ccgcttcgtc cgctgtgaat atggattgcg atatatcctg
     2581 cttcgtcata agtatatgga atcccaagct cttttgtcag atactctact gcccattgag
     2641 caatactgaa ttcatcactg taaagcactt cgatttctct taaaagtttg ttgcggatga
     2701 cgataccgtt attaatgttc tccgcagaaa aagcaatgtg gtcggtcaaa gcgatcaata
     2761 catgttcatt caacttttcc atcaaaacag tttccgcatg atcaatgatc gtttctgctg
     2821 caaataaaaa gcgttcatca atctgattaa gtaaattttg caacttgatt tgaccttctg
     2881 gttccatgac aaatatccgt tcgacatcac gatcaaaaat caaatcattt ctttttctgt
     2941 caaatccaat cccttttcca atagctactt tttcctgtcc accgtcatct accaatactg
     3001 cattttgatt aaagatcttt ttgattttca tgcgcgcacc ctttttgaag atttggtttt
     3061 cacgtttgct agaccagttg attgcggcca aacaaaaata gacacgaaaa acctcctagg
     3121 gaaaccatgt tcctcagaga tttttcatgc ctagtatgat ctagtcacat gaaacgaatt
     3181 attgttaacg taatcaattt tatatagaca gttatgtttt gtcaattctt tttttataat
     3241 tatagtaatt tactggaatt atcagaaata aaaggaagga acaaattaag aaacttatgt
     3301 tatttaatca ttggatcttt tgcttaactt attcgttgtt aaaaagctga aagcgaagga
     3361 aaaaaactct acttaactgt atttctccgt taggacatct aaaaatttac gagcagcagg
     3421 agaaagaggc tggtcttttt tccaaatgag attcagtgaa gcttgtaatg gcggatcgag
     3481 tgggatgaat actttttccg tcccataagt attgatgatc ccatctagag ctaatgcacc
     3541 aaaagtcccg ttatcgacca tcaaagaagc attgtacaat aagttatatg ttccgacgat
     3601 cgataactga tctatgctga aacctagcca ttcagctagt tgactgtcca ctgacgtttg
     3661 ccgggaaatg atcaaaggaa cagttttcag ttcctgcgga tggatgactg tttgctttgc
     3721 taaaggatga ttccgatgta tcaaaagtcc ccaacgatct tgggctggca gtcgaaatga
     3781 ctggtatttt tgtttgtttg tcggttcgat tacgagtcca aaatcaagta atccgctatc
     3841 cagtttgtct atgatatcat ctgcattccc gctatataaa tgaaactgga tatcaggatg
     3901 cgccttgatc aacgaatgaa ggctatcaga aatgaaagag attgctttag tttcgcctcc
     3961 gccaatgtag atttctccag agatgattgc gtcatttgtt aaattgttcg ccgttttttc
     4021 taccaacgac aagatttctt ttccttttga ataaagatat cgaccagaat ccgtcaactg
     4081 tatctctcgg gagcctcgag tgaaaagtgt cacaccaagt tcttcttcta aatctttcag
     4141 ttgttttgag agagtaggtt gcgtgatatg cagagatttt gctgctcggc tgatattttt
     4201 ttctctagct actgctaaaa aatacgtcag tatacgaata tccatttttt ccctcctgtt
     4261 tttaaaaaag cataccacta agtatagtta aaaggaatag ttaagtatgt tttataagta
     4321 ttagacatca taagccgtcc gttataaagt aaggataaaa ggagggagca aaatgacaag
     4381 cctcatcgaa aaacaaatcg tcaataaaga gattcgagta acggatccgc tctttgaagt
     4441 gatccatcag atccaagcgg aaaacgaaga gcctttagca gcgctgaata ctgggtacca
     4501 tgaaccagca gatatccgca aacggctaga aaatatcatt agtgacaaag tagatgatac
     4561 ggtgactgtt ctgctgcctt tttatacaga tttcggcaaa catattagta tagggaaaaa
     4621 tgtctttatc aatcgtcaag tgatgttcgt tgacttaggt ggcatctgtt tagaagattc
     4681 agtattgatt ggtcctcgtg tcaacctcat cacggtgaac catctaaccg atccaaaaga
     4741 acgaagaggc ttgtcagtaa agccaatcca catcaagaaa aatgcttgga tcggagcagg
     4801 agcaactatt ctgccaggcg taacgattgg cgagaatgcc attgttgcag cgaatgcaac
     4861 tgttacgaaa gatgtcccag acaataccat cgttgcagga atccctgcca aaatcgtcaa
     4921 acctgtcgaa cggattattt gaacagtaag atgaaataag agatagtgag tataagcaag
     4981 agatttttca aaacaggagg agaatagaat gaatgattta tcagaaaaag tgattgtcat
     5041 catgggcgct tcaagcggaa tcggtgaagc aacaacgaag ctattagcag aaaaaggggc
     5101 taaactagtt atagctgcac gccgagaaga tcgcctaaaa gcaataaaag aatctttacc
     5161 agaagctgag ctatatattc aaacagcaga tgtaagagat tttgcacaag tccaagcagt
     5221 tatcgattta gcgatggaaa agttcggtcg aatcgatgta ttgtacaata atgcaggaat
     5281 catgccaacc gctccattag ttgaaggtca ccgtgatgaa tggcaaaata tgcttgatat
     5341 caacatcatg ggtgtgttaa atggaatttc cgccgtactt ccaatcatgg aaaaacaaaa
     5401 atctgggcat atcatttcaa ctgattctgt ggcgggacat gttgtttatc ctgattccgc
     5461 cgtttattgc ggaacaaaat ttgctgtccg tgcaatcatg gaaggattac gtcaagagca
     5521 acggcaaaat aacatcaaat ctacgatcat ctctccagga gctgttcaaa ctgaattata
     5581 tcaaaccatc agcgacaaaa aggctgctct tgaacttcat gaagcgcaaa aagaatgggg
     5641 attaacatct gaagatatag cttctgctgt agcatttgcg atagaaacgc cagaccggat
     5701 gtctgtcagc aacatgatta tccgtccgac gacacaggaa gtataatcca taacggataa
     5761 tatccagaga gaattacttg ttaaatagtt attcaaacga ccatcaattt aaggcgtgaa
     5821 tggagaagaa tattcttcat ttacgtcttt ttatgcagaa aaagcaaatt tattaaaaag
     5881 aaacaaatgt tatttaaata tggacattga cttgctttta cttttttagt tacactgtta
     5941 tttaggaaaa gtttcataat tcaaaggaga aaaaagatga aagcaaagaa tttattattt
     6001 agcgttattc ttttaggaaa tctagcagtt gccaacacaa cagtgctagc tgaagaatcg
     6061 acagaaactg tcgcaactca gacatcgagt tcttcagaaa caggaattga aaacgataca
     6121 gaaagtactt tgcagtcagc tactgtctca gaaaatacaa tttcaaccga agaaccaaca
     6181 actgaaacaa cagaacaaac acaaaccgaa gacagtacat cagcagaagc tcagaacaat
     6241 ccagagaatg cagaagaaat cacgaaccaa ttgacagatg ggcaaacatt gacacatacc
     6301 catcaagcag atttgtctga attcgaaaac tatactggct atgcttttta tgctcgctca
     6361 tctgctaatt ctcaacaagc gttcattgat agtattgcct caactgcaca aagtttggca
     6421 agtgccaatg acttatatgc atcagtcatg attgctcaag ccatcgttga aagtggctgg
     6481 gggaacagta ctttggcttc tgcccctaat tacaatctat ttgggattaa aggaagctat
     6541 aatggccaat ctgttacgat gcctacttcg gaatatgtga atgggcaatg gattacggtg
     6601 aatgccgctt ttagaaaata tccttcgtac aaagaatcat tgcaagataa cgtaacggta
     6661 ttgaagacga cttcttttca gccaggcgtt tactactatt ccggtgcatg gaaaagcaat
     6721 acgaactctt acaaagatgc tacggcttgg ctaacaggtc gttatgccac tgcgcctaat
     6781 tatggttcta cattgaacaa tgtaatcgaa acctataatc tgacacagta tgatacagca
     6841 ccaacttcaa cttctccgat gtatcgtctg tataaccgtc atacaggaga acatttatat
     6901 acattgaatg ccggagaaaa aaattatctg ccaactgtag gctgggaata tgaagggatt
     6961 gcttggcaag caccaaacag cggacaacct gtctaccgtt tatacaatcc ttacagtgga
     7021 gaccatcatt acacaatggc acaatctgag atcaactctt taacaaaaat cggttggcgt
     7081 tatgaaggtc taagttttta ttctggtggt tccaagccaa tttatcgact atttaatcca
     7141 aatgaaaaaa ctggtaccca tcactatacc ttgagtgcta aggaaagaga ttggctaaca
     7201 ccgatgggct ggagatatga aggaatagga ttttatggtt attaaaaaga ggtagtgaaa
     7261 aagctgccct gcatcaagta gtaagaacag ccaaacaaaa acagattttc atgttttttg
     7321 tttggttgaa cttattaccg cagatgcaag tttttgaaca ccgtttactc aaaaaaggtt
     7381 gtgacaacat cttgtcataa cttttttatt actgtttaag catactgatc caatttctaa
     7441 tattcgacgg gattcttgca aaaggcgatt tcggaaattt ttggcggtgt aaaattatct
     7501 actgcagaag agctatcttc tatagtttga taagaatcaa taatcttggt tttagcgttg
     7561 tgctttcttt atcatttttt ccgttcattc tacaaaaaat ttcttttctc tatacttttc
     7621 tattgagagc gtttttttta ttacacagct gattctttga cttttttctt tttttattct
     7681 attattgaca agtcaatcgt tgacgagtca attattagaa aggaggaaat tatgtcttta
     7741 gaagagatta gtcacctttt atatcaaatc aaattagccg atcaaaaaat cgtccagatc
     7801 tttgaagaaa atatcggtat aagtcttact agatacgaga tcatgatgag actgaaagaa
     7861 gaaaatccat tgctacaaag cagtttgcaa gaaaagttgc agattgatca aggagcagtt
     7921 actcgtcatc tgaaggtatt ggaagcaaat ggctatgttt cgcgtcagag aaatcctcag
     7981 aacaaccggg aagtgtatgt agcgctaaca gaaaaagccc agaagaaaat caagcattgt
     8041 gaaatggacc ataaagatac aggttccgtg ctatccccgg ctttcagcga tgtagaaatg
     8101 aaacaatttc taacactttt agataaattg aatacccaac tttgcaatat aacaacagaa
     8161 acgaaagagg ttaataataa tggaacaaaa aaatgatttt tcaacaatta tgactggtag
     8221 aagatccgtt cgcgtatatg atgaaacata caaaatccca cacgaagaaa tgctggaaat
     8281 gatcaaagaa gcgacgactg caccttcttc tgtcaatatg aaaccatggc gctttgtggt
     8341 cgctgaaagc gaggaagcaa aagaaatctt gcgtcctttg atccgtttca atacgagaca
     8401 aaatgatact tcttctgcaa tggtcatgat ttttggcgat atgcaatgtt acgaatatgg
     8461 cgaagaaatc tacaaccaag cttacgaatc agggaagatg ccaaaagaag tcagagatca
     8521 acaattagct gcgatcattc cttattacaa gagtttttca agagaagaaa tgaacgatgt
     8581 agtcaaagtc gattctagct tagcggctat gcaattcatg ttagttgcgc gtgatcatgg
     8641 ctatgaaaca aatccaattg gtggattcga agcagatcag ttggcagagg catttggttt
     8701 ggataaagac cgttatgttc cagttattat tttatcaata ggaaaagcgg tagaagaagg
     8761 ttacgagtct gttcgtttag caccagaaaa gatcactatg ttcgaataaa aggttttcag
     8821 ttactattca gtagctattg ctttttttga atcgtgtgtg gtaacctaat aaaaaaggag
     8881 gtcggaaaaa tgactgcatc gatgaaatta cgtttcgaac ttttgaataa caactaaata
     8941 aattcaaaag acatgtataa acaacaattt catggatgga gtatgtccat tttttcgaac
     9001 gacagtagaa taggtgaaag ctatgacaga tttggtgatt tagtcatagt tatttcgttc
     9061 agagtaggaa aagatgtgat gcagtttttt tgcaaacaga tttttctgct cttttttagt
     9121 atcgataatt gttgttttac atgaaatagg agtgtaaaac atatggaaaa tttagcagta
     9181 aatataacaa atctacaagt cagctttggc aaccagctag aactatctat tgattctctt
     9241 cgtgtctatc agcaagatcg tatagggatc ataggagaaa atggtgtcgg taaatcgact
     9301 ttgctcaaac taatagccgg tgaacttttt cccgatcatg ggaaaatcca aacagagatc
     9361 accttcaact acctgcctca attaacctat ctcgctgaag caaaggacct aaatttggaa
     9421 ttagccagtc atttccagtt aagactggaa gaaacttcag agcggaaatg gagcggaggg
     9481 gaagaacgaa agatcgagtt gatacgtctt ctttcttctt atgaacaagg gatgcttcta
     9541 gacgagccaa caacccatct agatagaaaa agtattgatc gactgattga agagcttcgt
     9601 tattattatg gtacgctggt ttttgttagt catgatcgct attttctaga tgagctagca
     9661 tcgaaaatct gggaagtaaa agacggagaa atccgagagt tttcggggaa ttatagtgcc
     9721 tatctcactc aaaaggaatt ggagaaaaag actcagctac gagaagcaga gtcgatcatg
     9781 aaagagaaaa aacgattgga aaaatcgatc caagaaaaga aaaaacaagc ggaaaagttg
     9841 gaaaaagtgt ccagtaaaaa gaaaaagcaa caaatcagac tggatcggtt gtcttcctct
     9901 aaacaaaaag acagtgtaca aaaagccatc caaaagaatg cgaaaacatt agagagaaga
     9961 ctccaaaaaa taggagaaac aaccaagccg cagcagatga aacaaatccg ttttccagta
    10021 ccaaaatctc ttgaactcca cagccgctat cccatcatgg gacaaaatgt ccaattggaa
    10081 agaagtggga gaacattact ggtaaatggt gattttcagt tttctttagg taaaaaaatc
    10141 gcgattgtcg gtgaaaatgg ttcaggtaag acaaccttat tagaacatat ccgcaaacaa
    10201 ggagaaggaa tccttctctc tccgaaagta agctttcaag tatatcagca aaagggttat
    10261 caaatgacat ctgaagaatc catcattcgt tttgtcatga gacaaacaga gttttcagaa
    10321 tcgcttgtcc gtagtttgct gaatcactta gggtttgctc aggaaacttt gacgaaaccg
    10381 ttatgtacat taagtggggg agaagcgacc cgtctgacga ttgctttgct ttttactaag
    10441 ccaagtaatg tgttgctgtt agatgaaccg actaatttta ttgatatggc aacgatcgaa
    10501 gctttagaga agctaatgca aatatatccg ggaacgattt tgtttacttc tcatgattct
    10561 tactttgtcg agcgtacggc tgatgaagtt tatgaaataa aagggcagaa aataaaaaaa
    10621 gtacttacga gaaattttta aatgtgctac gatgattttc gtgacaaatt taaaggagtg
    10681 gtacagattg cctacaaata aaaaagaagg aataattttt acaacaatga tgtgtttttt
    10741 gatggtcttg gggatgagcg cgtataattt atggctgcat caagatcttc atttatcaag
    10801 tttgttgatt ggtctagtgc ctggattcgt ggtggctttc attttggatg tcttcatcat
    10861 cggtgtgctg gcgaaaaaaa ttgcctttaa actaccagta aatccagaaa gcaaggtcca
    10921 aatgatctta actatttcat gcttgatggt tttaggaatg gtgacatgta tgtctttatt
    10981 cggtgtgatc atggaacaag gaatacctga tcaattattc tctgcttata tgaatgcttg
    11041 gaaaatgaat gtgattgccg ctttaccgtt gcagttgatc atcgttggcc cgctttcaag
    11101 aagtgtgcta aaagcaattc aaagtaatga atgatggaac actgttattt gaaaaaaggc
    11161 cttggcatgc tttttgtcat agtctcttga atgcagttct agaaaaatag tgatccgaac
    11221 gataggtttt ggcctgtcgt tcggatcgct ttttcttttc caactgtatt gctcttttat
    11281 tgaagataat ttgctcttca atagatattt ctagaaaggt tctgtttatc ccttactcaa
    11341 aaaactgctg catttgttct cttacttgct gacaccattg gagttcctgc tgacaaagca
    11401 taattctgcg ctggatcaca acataacgtc caaattcttg tttcttgtct agcgagaggt
    11461 cattcaattt tttttgattt ttttgcatga ttttgttcag ctgttgttct ctagtgaata
    11521 gcagtccagt agctgttgtt ttatcgagcg tcgaaaaagc atagattttt gctagaaatt
    11581 catctttttg cgtaggagcg ggagtttcct ctttgatcca ttcctctaca agagcacgtc
    11641 cttcatcagt caagtggtag atcttttttt gagagtgctc gtctttgtcc aaaacggtca
    11701 cataaccctg ctggtctaac ttggcaagcg ttgtatagat ctgactgtga tgggcttccc
    11761 aaaaaattgt gatcagctgc ttcaattcat acccagaacg tggtttggat aaaagcacgc
    11821 ataataatat ataggctaaa gtattcataa aaatcctacc tctcgtctta ttttctttat
    11881 tatatcacag taaagtttcc acgttttaca tgtgcatatt gacatgtaaa ataaatcatg
    11941 tatgatgcta atagagagaa agggatggaa atattggaga atcaaataga tatacaagaa
    12001 caaacaaatg aagaaacaaa ggggaaaagt tcagcgctag ttgtagcagc tacctgtatg
    12061 atcgctttta tgggagtggg tctagtagac ccaatcttaa aaacgattgc tgcacagtta
    12121 aacgctacgc cagctgaaac gaccctgtta tttactagtt acatgttagt cactggtgtg
    12181 gtgatgctat ttacaggttt cttatctagt cggatcggtt tgaaaaaaac attgatgaca
    12241 ggattgttta tcatcgttgt attcgctggt cttggcggat tatctgggaa tatcggaatg
    12301 ctgattgggt tacgtgccgg ctggggtgtc gggaatgctt tatttgtctc tactgcttta
    12361 gcaacgatcg tcagtatttt agttggtaac acagaaaaag caatcatgat gtatgaagca
    12421 gcacttggct gcggaatggc tgttggccct ctagtcggtg gggtcttggg cagtggttca
    12481 tggcgttatc cattttttgg ggtagcattt ttgatgtttt gcggttttat agccctagcg
    12541 gtacttttac ctgaaactga gaaaccaaag agaaaagtag gcctttggga aggtgtcaaa
    12601 gcgttaagca atcataaact tcgttcagtt ggactaatcg cattgttata taatttcgga
    12661 ttctttactt tacttgcgta tgcgccattt ttattgattg gctattctga attagaagtc
    12721 ggttttgtat tttttggctg gggtgtgctt ctggctattg cttcgatttt cgttgcaccg
    12781 gctcttgagc ggaagtttac gacttatcgg acaattttag cagcatttgt cttattcatt
    12841 atttgtttgc tgttattagg agctggaact tctatgccag tccttgtacc gatcagcatt
    12901 gtgttagcag gatttttcca aggaattatc aatacactgt tgacgacgat tgctatggaa
    12961 ataccaggtc tgcaacgaaa cgttgcttcg tccagctata gttttgtccg ctttttcggt
    13021 ggagctttag caccatttat tgcaggtgtg attggtgaac atattggtga aagctatgcg
    13081 ttttattttg caggtgccat tgttcttatc agtgtatgtt gtatcattta tcaccgtaat
    13141 tattttgcta caaatgaaga agaataagaa atcagaggtt gtgaaaaagc tgtcctgcat
    13201 caagtcataa gaa
//
LOCUS       DAZKLM010000271        10793 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3764
            SAMEA115706913-rid22582563.guided.093, whole genome shotgun
            sequence.
ACCESSION   DAZKLM010000271 DAZKLM010000000
VERSION     DAZKLM010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706913
            Sequence Read Archive: ERR13196647
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10793)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10793)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287823.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287823.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,004
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,849
            CDSs (with protein)               :: 2,849
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 41 of 96
            Pseudo Genes (incomplete)         :: 52 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 15 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10793
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706913-rid22582563.guided.093"
                     /isolate="VRE3764"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(1..998)
                     /locus_tag="ACG842_002973"
                     /pseudo
     CDS             complement(1..998)
                     /locus_tag="ACG842_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108743.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
     gene            1489..2427
                     /locus_tag="ACG842_002974"
     CDS             1489..2427
                     /locus_tag="ACG842_002974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HFQ0249395.1"
                     /translation="MKKIDEKLAAEISLKKPAVSNSRLSNSRLSGQTLGKSQPALSFD
                     AMTIVGNLNKTNAKKLSDFMSVEPQIRLWDILQTKFKAKALQEKVYIEYDKVKADTWD
                     RRNMRVEFNPNKLTHEEMIWLKQNIIDYMEDDGFTRLDLAFDFEDDLSDYYAMTDKAV
                     KKTVFYGRNGKPETKYFGVRDSDRFIRIYNKKQERKDNADVEVVSEHLWRVEVELKRD
                     MVDYWNDCFNDLHILKPAWATLEKINEQAMVYTLLHEESMWGKLSKNTKTKFKKLIRE
                     ISPIDLTELMKSTLKANEKQLQKQIDFWQREFRFWK"
     gene            2557..3204
                     /locus_tag="ACG842_002975"
     CDS             2557..3204
                     /locus_tag="ACG842_002975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type A-7 chloramphenicol O-acetyltransferase"
                     /protein_id="HFQ0249396.1"
                     /translation="MTFNIIELENWDRKEYFEHYFNQQTTYSITKEIDITLFKDMIKK
                     KGYEIYPSLIYAIMEVVNKNKVFRTGINSENKLGYWDKLNPLYTVFNKQTEKFTNIWT
                     ESDKNFISFYNNYKNDLLEYKDKEEMFPKKPIPENTIPISMIPWIDFSSFNLNIGNNS
                     SFLLPIITIGKFYSENNKIYIPVALQLHHSVCDGYHASLFMNEFQDIIHRVDDWI"
     gene            complement(3897..4835)
                     /locus_tag="ACG842_002976"
                     /pseudo
     CDS             complement(3897..4835)
                     /locus_tag="ACG842_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108743.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
     gene            complement(4835..5452)
                     /locus_tag="ACG842_002977"
     CDS             complement(4835..5452)
                     /locus_tag="ACG842_002977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFQ0249397.1"
                     /translation="MAKIGYARVSSKEQNLDRQLQALQGVSKVFSDKLSGQSVERPQL
                     QAMLNYIREGDIVIVTELDRLGRNNKELTELMNQIQIKGATLEVLNLPSMNGIEDENL
                     RRLINSLVIELYKYQAESERKKIKERQAQGIEIAKKKGKFKGRQHKFKENDPRLKHAF
                     DLFLNGLSDKEVEEQTGINRRTFRRYRARYNVTVDQRKNNEKRDS"
     gene            complement(5466..5636)
                     /locus_tag="ACG842_002978"
     CDS             complement(5466..5636)
                     /locus_tag="ACG842_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000713595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0249398.1"
                     /translation="MKKFIYRVLENDEVVAIFNEQQYAQDFIAYEKTISDKQFEIEKV
                     DIADWLLQPREF"
     gene            complement(5984..7474)
                     /locus_tag="ACG842_002979"
     CDS             complement(5984..7474)
                     /locus_tag="ACG842_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015543625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primase C-terminal domain-containing protein"
                     /protein_id="HFQ0249399.1"
                     /translation="MNIPFVVETVLHDGLLKYKFKNSKIRSITTKPGKSKGAIFAYRS
                     KSSMIGGRGVVLTSEEAIQENQDTFTHWTPNVYRYGTYADENRSYTKGHSENNLRQIN
                     TFFIDFDIHTAKETISASDILTTAIDLGFMPTMIIKSDKGYQAYFVLETPVYVTSKSE
                     FKSVKAAKIISQNIREYFGKSLPVDLTCNHFGIARIPRTDNVEFFDPNYRYSFKEWQD
                     WSFKQTDNKGFTRSSLTVLSGTEGKKQVDEPWFNLLLHETKFSGEKGLIGRNNVMFTL
                     SLAYFSSGYSIETCEYNMFEFNNRLDQPLEEKEVIKIVRSAYSENYQGANREYITILC
                     KAWVSSDLTSKDLFVRQGWFKFKKKRSERQRVHLSEWKEDLMAYISEKSDVYKPYLVT
                     TKKEIREVLGIPERTLDKLLKVLKANQEIFFKIKPGRNGGIQLASVKSLLLSIIKVKK
                     EEKESYIKALTNSFDLEHTFIQETLNKLAERPKTDTQLDLFSYDTG"
     gene            complement(7517..7816)
                     /locus_tag="ACG842_002980"
     CDS             complement(7517..7816)
                     /locus_tag="ACG842_002980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0249400.1"
                     /translation="MNHDECKTYIKNSLLEIRKLANIYTLETFKKELEKRNIYLETKS
                     DKYFSSEGEDYIYKLIENNKIIYSISGKKLTYKGKKSFSKHAILKQLNEKANQVN"
     gene            complement(8182..8466)
                     /locus_tag="ACG842_002981"
     CDS             complement(8182..8466)
                     /locus_tag="ACG842_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFQ0249401.1"
                     /translation="MELAFRESLKKMRGTKSKEKFSQELEMSRSNYSRIESGKSDPTI
                     KTLEQIAKLTNSTLVVDLIPNEPTEPEPETESEQVTLDLEMEEEKSNDFV"
     gene            complement(8985..9470)
                     /gene="vanZ-A"
                     /locus_tag="ACG842_002982"
     CDS             complement(8985..9470)
                     /gene="vanZ-A"
                     /locus_tag="ACG842_002982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000516404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance protein VanZ-A"
                     /protein_id="HFQ0249402.1"
                     /translation="MGKILSRGLLALYLVTLIWLVLFKLQYNILSVFNYHQRSLNLTP
                     FTATGNFREMIDNVIIFIPFGLLLNVNFKEIGFLPKFAFVLVLSLTFEIIQFIFAIGA
                     TDITDVITNTVGGFLGLKLYGLSNKHMNQKKLDRVIIFVGILLLVLLLVYRTHLRINY
                     V"
     gene            complement(9623..10534)
                     /locus_tag="ACG842_002983"
     CDS             complement(9623..10534)
                     /locus_tag="ACG842_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala carboxypeptidase VanY-A"
                     /protein_id="HFQ0249403.1"
                     /translation="MKKLFFLLLLLFLIYLGYDYVNEALFSQEKVEFQNYDQNPKEHL
                     ENSGTSENTQEKTITEEQVYQGNLLLINSKYPVRQESVKSDIVNLSKHDELINGYGLL
                     DSNIYMSKEIAQKFSEMVNDAVKGGVSHFIINSGYRDFDEQSVLYQEMGAEYALPAGY
                     SEHNSGLSLDVGSSLTKMERAPEGKWIEENAWKYGFILRYPEDKTELTGIQYEPWHIR
                     YVGLPHSAIMKEKNFVLEEYMDYLKEEKTISVSVNGEKYEIFYYPVTKNTTIHVPTNL
                     RYEISGNNIDGVIVTVFPGSTHTNSRR"
BASE COUNT         3335 a         1878 c         1671 g         3909 t
ORIGIN      
        1 aatacaatat tgttgccaca cttaggacat tttcctacaa tacttttttc tgcttctttt
       61 tctttctgtt cctggtaatc agaaaaattt agtttttcta tatcgttagg tacagcttcc
      121 agtaaatgaa caatgaattt tttgatattc gtaataaagt tctcttgatt gccttctctt
      181 ttaccgattt tttttaaata cgtttcccat ttagccgtca tttcagcact cgttaaaagg
      241 tgctgacttt caactgcctg gcacaataat tttccttttt cagttacaac aagcttattc
      301 ttaatcactt ggatatattc tttttgtttt aacgcttcaa taatacttgc tcttgtcgct
      361 tctgttccaa tcccctcaac ttcttgtaaa atcttgattg cttcttcatc atcaaccgtt
      421 ttattcgccg ttttcatagc agttaataat gtaccctctg taaaagcttt tggcggttgc
      481 gtttcttttt caacactctt aacaccaact tcggcacgtt cgccaatgat aacgagtggt
      541 aacgtttgaa cgtcctcttt ttcttctttg gtttgttgtt tgaaaagaat tttccaccct
      601 tcttgctttg gtgtctttcc gatcgattga aacagcaaat cagctacttt cgtttgtatc
      661 ttggtttctt catacaagta atcaggtaaa aacatggcaa cggtcgtttt aacgactaaa
      721 gcataaattt ttcgttgtaa atcgtccatt ttcgctaatg cagattcggt aggtacttgt
      781 tttgttggga taatggcgtg gtgttcctgt accttactac cgtccacata acgctttcta
      841 ggctctgttt gaaccatttc aagatcaagt cctaaaaaac cgctatattt gccaaaattc
      901 gcttttaaat aagcaaattc gttctctgta ataaatggtg tatctgttct tggataactc
      961 aataattttg cttcatacag tccttgcata gcttttaaac gtaaaatttt gcgttctaag
     1021 acgttttaaa tatatctgcg tatattttta tgtgttttat cttttcgtcg gcaaatgcgt
     1081 taaaatttgc gaaataaaat agctcaaggg cgaacgcagt gagtgcattt acccttgatc
     1141 tatttttttg agtaaattag cttttgaaac cgacggaaag gaaacacaaa acttttcaga
     1201 ggcaaggggt ttttaagggg cgcttttagc cccttgaatt tatttgcttt aagatatatt
     1261 tagttttaaa atagttttaa cgaaaaaaag acataaaaaa agacgagcaa cgctaccaac
     1321 gttctcgccc ggaataacaa accacacttg ttattcattc gatgattagt atatcaattt
     1381 ttgattttga atacaaaaca aaatggacga atggatacag gcgaaattta ttttgcgatt
     1441 ccactcgtcc ttttttcgtt ccaaaaatta ttttaggagc gataaaaaat gaagaaaatt
     1501 gatgaaaaat tagctgcaga aatctcacta aaaaaaccgg ccgtctctaa tagccggtta
     1561 agtaatagcc ggttaagtgg tcaaactttg ggaaaatctc aacccgcatt aagttttgat
     1621 gccatgacaa tcgttggaaa tttgaacaaa actaatgcta aaaagctatc tgactttatg
     1681 agtgtagagc cacaaatacg actttgggat atacttcaaa caaagtttaa agctaaggca
     1741 cttcaagaaa aagtttatat cgaatatgac aaagtaaaag cagatacttg ggatagacgt
     1801 aatatgcgtg ttgaatttaa tcccaataaa ctcacacatg aagaaatgat ttggttaaaa
     1861 caaaatatta tcgactacat ggaagatgac ggttttacaa gattagactt agcttttgat
     1921 tttgaagatg atttgagcga ttactatgca atgactgata aagcagttaa gaaaactgtt
     1981 ttttatggtc gtaatggcaa gccagaaaca aaatattttg gtgtccgtga tagtgataga
     2041 tttattagaa tttataataa aaaacaagaa cgtaaagata acgcagatgt tgaagttgtg
     2101 tctgaacatt tatggcgtgt agaagttgaa ttaaaaagag atatggttga ttactggaat
     2161 gattgtttta atgatttaca catcttgaaa cctgcgtggg ctactttaga aaaaattaat
     2221 gagcaagcta tggtttatac tttgttgcat gaagaaagta tgtggggaaa gctaagtaag
     2281 aatactaaga ctaaatttaa aaaattgatt agagaaatat ctccaattga tttaacggaa
     2341 ttaatgaaat cgactttaaa agcgaacgaa aaacaattgc aaaagcagat tgatttttgg
     2401 caacgtgaat ttaggttttg gaagtaaaat aagttttatt tgataaaaat tgctaattca
     2461 gtataattaa tatttacgag gtgacataac gtatgaaaaa atcagaggat tattcctcct
     2521 aaatataaaa atttaaaatt taggaggaag ttatatatga cttttaatat tattgaatta
     2581 gaaaattggg atagaaaaga atattttgaa cactatttta atcagcaaac tacttatagc
     2641 attactaaag aaattgatat tactttgttt aaagatatga taaaaaagaa aggatatgaa
     2701 atttatccct ctttaattta tgcaattatg gaagttgtaa ataaaaataa agtgtttaga
     2761 acaggaatta atagtgagaa taaattaggt tattgggata agttaaatcc tttgtataca
     2821 gtttttaata agcaaactga aaaatttact aacatttgga ctgaatctga taaaaacttc
     2881 atttcttttt ataataatta taaaaatgac ttgcttgaat ataaagataa agaagaaatg
     2941 tttcctaaaa aaccgatacc tgaaaacacc ataccgattt caatgattcc ttggattgat
     3001 tttagttcat ttaatttaaa tattggtaac aatagcagct ttttattgcc tattattacg
     3061 ataggtaaat tttatagtga gaataataaa atttatatac cagttgctct gcaacttcat
     3121 cattctgtat gtgatggtta ccatgcttca ctatttatga atgaatttca agatataatt
     3181 catagggtag atgattggat ttagttttta gattttgaaa gtgaatttaa ttttatacac
     3241 gtaagtgatc ataaaattta tgaacgtata acaaccacat tttttggttg cttgtggttt
     3301 tgattttgaa tttggttttg aacttatgga ctgatttatt cagtccattt tttgtgcttg
     3361 cacaaaaact agcctcgcag agcacacgca ttaatgactt atgaaacgta gtaaataagt
     3421 ctagtgtgtt atactttact tggaagatgc accgaataaa aaatattgaa gaacaactag
     3481 caaaagattt taaagagtta ttttatttta agtctttata acatgagtga agcgaatttt
     3541 taaatttcga tagaaatttt tacatcaaaa agccccctgt caaaattgac gaagggggtt
     3601 ttttggcgca cgcttttcgt tagaaatata caagattgaa aatcgtgtat aagtgcgccc
     3661 tttgttttga acttagcacg ttacatcaat tttttaaaat gatgtataag tgcgcccttt
     3721 taaattttga gtgattatat tttttgagtt agaaaaaggg attgggaaaa tttcccaaaa
     3781 taatttaaaa aataagcaaa aattttcgat agagaatgtg ctattttttg tcaaaggtgt
     3841 ataccttgac tgtgcttgct gttacattaa gtttattttt aagttattaa aaaagaaata
     3901 gcttttaaag tttggctcgc tgtcgcttta taaagctgat tgacttttga ttgcaaacta
     3961 cttaaagaaa acaaactcgg actattcgtt ttcttctctt tggtttgaac atcagcaatt
     4021 atcccctctt gattgcctat tttagcttgt ttagaagaaa caaaagctaa aagctcctct
     4081 tgggttttaa aacgctgtgt ggggcttaga acgcccttaa acgacccttg gtttactttt
     4141 atactagctt ccacctcgaa aaaaggttct tttttaaaat tctctatggc ttcctggcgc
     4201 tgaaaaataa ggtataaggt gggcgtttga acacgtccta gtgaaaatgt accttgtacg
     4261 cccttctgtt gtaaatttaa cgtatacaaa gggcttgcgt tcatgccgat caaccaatcg
     4321 gcaatttggc gtgtttgcgc ttcttgataa aagggatagt aattcattcc aggttgcaaa
     4381 ttttgaaaac cgcttcggat tacatctttt tctaagctat tgatccatag tcttttaaat
     4441 gttttatctt ttgaaaaggc atttgcttta tggataatcg accaggcgat attttcacct
     4501 tctctgtcgc tatctgttgc aacaataatt gtatttgcct ttttgagaag ttctgcaaca
     4561 attttaaact gctttccctt atcttttgca acttcaaaat cgtatcgatc aggaaaaatc
     4621 ggcaaagatt caagtttcca attttgccac ttttcgtcat aatgacctgg ttctgctaat
     4681 tccactaaat gcccaaaacc aaaggtgata aacgtttcat ctgtaaatag tgggtctttg
     4741 atctcaaaat aaccgtcttt tttggtgctt tgttttaaag cacttgcgta ggctaatgcc
     4801 tggcttggtt tttcagctaa aataaccgta ctcattaact atccctcttt tcattgtttt
     4861 ttctttgatc gactgtcacg ttatatcttg ctcgatacct tctaaacgtt cggcgattga
     4921 ttccagtttg ttcttcaact tctttatcgg ataaaccatt caaaaacaaa tcgaaagcat
     4981 gctttaaacg tggatcattt tctttaaatt tatgctgacg acctttgaat ttgccttttt
     5041 tcttagcaat ttcgattcct tgtgcctgac gttccttaat tttttttcgt tctgattctg
     5101 cttgatactt gtacaattca atgacaaggc tattaatcaa acgccttaaa ttttcatctt
     5161 caataccatt cattgagggt aaatttaaga cttccagggt tgccccctta atttgaattt
     5221 gattcatcaa ttctgttaat tctttattat ttcgtcctaa tcgatctaat tcagtaacaa
     5281 taacaatatc cccttcacga atatagttaa gcatagcttg taattgtggg cgttcgaccg
     5341 attgaccgct taatttgtct gaaaagacct tagaaacgcc ctgtaacgct tgtaattgcc
     5401 gatctaagtt ctgttctttg ctactgacac gtgcataacc aattttagcc attttcaacc
     5461 aacctctaaa attctctcgg ttgcaataac caatcagcaa tatctacttt ttcaatttca
     5521 aattgcttat cagaaattgt cttttcgtaa gcgataaaat cttgcgcata ttgttgctca
     5581 ttaaaaatag ccaccacttc gtcattttct aaaactcgat aaataaattt tttcatttta
     5641 ctcctcctat tatgcccaac ttaaatgacc tattcaccaa gtcaattata ctgctaaaat
     5701 catattagga caaataggta tactctaatg acctataaaa gatagaaaat taaaaaatca
     5761 agtgttcgct tcgctctcac tgcccctcga cgttttagta gcctttccct cacttcgttc
     5821 agtccaagcc aactaaaagt tttcgggcta ctctctcctt ctccccctaa taattaatta
     5881 aaatcttact ctgtatattt ctgctaatca ttcgctaaac agcaaagaaa aaacaaacac
     5941 gtatcataga tataaatgta atggcatagt gcgggtttta ttttcagcct gtatcatagc
     6001 taaacaaatc gagttgtgtg tccgttttag ggcgttctgc tagcttgttt aaagtctctt
     6061 gaatgaatgt atgctctaag tcaaaagaat ttgtcagcgc ctttatatag ctttcttttt
     6121 cttctttttt tactttaatg atcgatagca acaatgattt aacactagca agttgaatgc
     6181 caccatttct tcctggttta atcttaaaga aaatttcctg attcgccttc agtaccttca
     6241 gcaatttatc taatgtccgt tcaggaatgc ctagcacttc tctaatctct tttttggtcg
     6301 tcactaaata aggcttgtat acatcgcttt tttcgctaat ataagccatt aaatcttctt
     6361 tccattctga caaatgaaca cgttgacgtt cgcttctttt tttcttgaat ttaaaccacc
     6421 cttgacggac aaataaatct ttactggtta aatcacttga tacccaagct ttgcaaagaa
     6481 tggtaatgta ttccctatta gccccttgat agttttctga ataggcactt ctaacaattt
     6541 tgattacttc tttttcttct aagggttgat ctaatcgatt attaaactca aacatattat
     6601 attcgcacgt ttcgattgaa tagcctgaac taaagtaggc taaagagagg gtaaacatga
     6661 cgttattacg ccctattaaa cccttttctc ctgaaaattt cgtttcgtgc aataagagat
     6721 taaaccaggg ttcatctact tgttttttgc cttctgtacc gcttaaaacc gttagacttg
     6781 aacgagtaaa gcccttatta tctgtttgtt tgaaagacca atcttgccat tctttgaaag
     6841 aataacggta attaggatca aaaaattcta cattgtccgt tcttggtatg cgagcaatac
     6901 caaaatgatt acacgttaga tcaactggca aagactttcc aaaatattct cggatatttt
     6961 gcgaaattat tttggctgct ttgacagatt taaattctga ttttgaagtc acatagactg
     7021 gcgtttctaa aacaaaatat gcttgataac ctttatcaga tttgataatc atagtaggca
     7081 taaaacctaa atcaatagcg gttgttaaaa tatcgcttgc tgaaatagtt tcttttgccg
     7141 tgtgaatatc aaaatcaata aagaaggtat tgatttgtct taaattgttt tcagaatgtc
     7201 ctttcgtgta tgaacggttt tcgtctgcat acgttccata acgataaacg ttgggtgtcc
     7261 aatgtgtaaa tgtatcttga ttttcttgaa tcgcttcctc ggaagtcaga acaacaccac
     7321 gaccgccaat catgcttgat tttgagcgat acgcaaaaat agcccctttg cttttacctg
     7381 gcttggtagt gattgagcga attttactat ttttaaattt gtactttaac aagccgtcat
     7441 gaagcacagt ttctacaaca aaagggatat tcattcagct gttctccttt cctataaatc
     7501 ctataaaata ggttgtttaa ttaacttggt ttgctttttc attcaactgt ttcaatattg
     7561 catgttttga aaaagatttt tttcctttat aagtcaattt ttttccacta atcgaataaa
     7621 ttattttgtt attttctatt aacttatata tataatcttc cccctccgaa gaaaaatact
     7681 tatctgattt tgtttctaag tagatatttc tcttttctaa ctctttctta aacgtttcta
     7741 gtgtatagat atttgctaat tttcttatct ccaataaact attttttata taagttttac
     7801 attcatcatg attcatacaa actccacctt ctataaatga atacaaaaaa agcaatcaaa
     7861 cgatttccga ttgattgctt aacaattctt aaattcagta gcttagatac ttgaaaactc
     7921 tctgatttcc ctatataatg atagtacggt tatataccgt cttcaaacaa agttaattaa
     7981 ataacttctt acgagggaag agttcatctg actaactgat aagcgttggt ttggcaatct
     8041 tatcgggcta tgcatttata aaatgtcgtc aaacatttta taaatgtgtc atggctcttt
     8101 tttcgtttct attcagttcg ttgtttcgtt atatctagta taccgctttt aaaaaaaata
     8161 agcaacgatt tcgtgcatta ttcacacgaa gtcattgctt ttttcttctt ccatttctaa
     8221 atccaatgtt acttgttctg attctgtttc tggttctggt tctgttggct catttgggat
     8281 taaatccact actagcgttg agttagttaa ctttgcaatt tgttctagtg tttttatggt
     8341 tggatctgat tttcctgatt ctattcgtga ataatttgat ctactcattt ctaattcttg
     8401 ggagaatttt tcttttgatt tggtacctct catctttttt aagctttctc taaatgctag
     8461 ttccaagttt ctcccccctc tttttgtgta ataaaattca cgctttattt aaattatagc
     8521 ttttaatatc ttaataaaca agtgattaat cacttgtgat ttgtgattat tccaaaagta
     8581 ggtaatttca ccaacctttt tcaaaagttt ttatcgacga ttattggact ggatcgtgtt
     8641 tcctaaagcg attctaagcg catgtttaat tttaaagtgg gtaattctct ttaaaacgtc
     8701 ctacaaagat tcaaaaaaag catggctgct agtgggggta ccgtcaggaa aatgcggatt
     8761 tacaacgtta aggaaattta ttaaagagac caattcctca gcactaaaat tgataattgg
     8821 gctttctttg ttactatagt gttaggtaag ctttttactt tatcaccggt tttcccctca
     8881 cttcacacct acgggcgagt ttccccacat cccgttttcc atctaatcca attcattcaa
     8941 tatctttatg tgtatgaaag atcagattgc ttgatttaga catcttacac gtaatttatt
     9001 cttaaatggg tacggtaaac gagcaataat acgagcaaaa gtatacctac aaaaataata
     9061 actctgtcta attttttttg attcatatgc ttattgctta aaccatataa tttcagtcca
     9121 agaaagcctc caacagtatt tgtaattaca tctgttatgt ctgtcgctcc aatagcgaag
     9181 ataaattgaa ttatttcaaa agtaagactt aaaaccagta caaaagcaaa cttaggtaaa
     9241 aatccgattt ctttaaaatt gacattcaaa agcaagccaa atggaataaa gattataaca
     9301 ttatctatca tctctctgaa attcccagta gcagtaaatg gagtcaagtt aagacttctt
     9361 tgatgataat taaatactga taaaatattg tattgtaatt tgaataacac taaccagatt
     9421 agtgtcacta aatataaagc tagcaatcct ctagataata tttttcccaa tataatttcc
     9481 tctaattctt ttgtttaagt tgtatcatta gtatagcaat gtcctagtta acgtgggttt
     9541 taaattaaaa agctaacgtt accccaaaag tgctagtaca ataatgctgt tcattaattt
     9601 cgttggtttc attccgccat ccttacctcc ttgaattagt atgtgttgat ccgggaaaca
     9661 ctgtcacaat tacaccgtct atattgtttc ctgatatctc ataacgaaga ttagtcggca
     9721 catgaatggt ggtattttta gtaacaggat aataaaagat ctcatatttt tccccattta
     9781 cactaacaga aatggttttt tcttctttta ggtaatccat atattcctcg agaacgaaat
     9841 tcttttcttt cataatcgca ctatgtggta aaccaacata gcgaatatgc catggttcat
     9901 attgaattcc tgttaactct gttttgtcct ctggataacg taaaatgaac ccgtatttcc
     9961 aagcattttc ttctatccac tttccttcag gggctcgttc cattttcgtc aagcttgatc
    10021 ctacatctag tgataaacct gaattatgct cactataacc tgctggtaag gcatactcag
    10081 cccccatttc ttggtaaagc acactttgct catcaaagtc tcgatagcca ctattaataa
    10141 taaaatgact aacgccaccc tttacagcat cattgaccat ctctgaaaat ttttgtgcta
    10201 tttcttttga catataaata ttactatcaa gcaacccgta tccatttatt aattcgtcat
    10261 gtttagataa attcacgata tctgacttca cactttcttg gcgaacagga tatttactat
    10321 tgattaatag cagatttcct tgataaacct gttcttctgt aattgttttc tcttgggtat
    10381 tttcagaagt cccactattt tctaaatgtt ctttgggatt ttgatcataa ttttgaaatt
    10441 cgactttttc ctgagaaaac agtgcttcat taacgtagtc ataacctaag tatattaaga
    10501 ataacaataa caataaaaaa aacaacttct tcattttcag tctcctcctt aactatatag
    10561 ttggaagtat gaaatatttc tagaatcttc cccttaaacg gcacaatctc gtttgagatt
    10621 gtgccgtttt gccccgtaaa ttacagtcaa ttatttcgcc ctactggatt tactattatc
    10681 accaatgtag atgaattttc ataagtgata cccatgctga atttaacgag gctgaactgc
    10741 ctgttgacaa tgggggttct gttgcaaagt tttaaatcta ctatcaaata agg
//
LOCUS       DAZKLM010000272         8062 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3764
            SAMEA115706913-rid22582563.guided.112, whole genome shotgun
            sequence.
ACCESSION   DAZKLM010000272 DAZKLM010000000
VERSION     DAZKLM010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706913
            Sequence Read Archive: ERR13196647
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8062)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8062)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287823.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287823.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,004
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,849
            CDSs (with protein)               :: 2,849
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 41 of 96
            Pseudo Genes (incomplete)         :: 52 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 15 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8062
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706913-rid22582563.guided.112"
                     /isolate="VRE3764"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            128..1564
                     /locus_tag="ACG842_002984"
     CDS             128..1564
                     /locus_tag="ACG842_002984"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFQ0249404.1"
                     /translation="MEHKELTSFPKDFLWGSASAAYQVEGAWQEDGKGESVWDRFVRI
                     PGKTFKGTNGDLAVDHYHRYKEDIALMKEQGLKAYRFSVAWTRIFPNGRGEVNQAGLA
                     FYERLIDELIENDIEPMLTLYHWDLPQALQDEYNGWESRQIIEDFTHYAETLFEAFRG
                     KVHYWISLNEQNIFTSLGYLLAAHPPGVTDPKRMYEVNHIANLANASVINKFHELEMP
                     GKIGPSFAYTPNYPIDSNPENVLAAENAEDLMANYWMDVYMWGKYPIAAMRFLEEKGW
                     APTIEAGDAELLESAKPDFLGINYYQTATNAFNPLNGVGAGKMNTTGKKGSSEETGVP
                     GMYKKVENPFVERTNWDWEIDPEGLRIGLRRITSRYRMPVLITENGLGEYDKLTEDKQ
                     IHDDYRINYLQSHVKAIKEAISDGAEVLGYCTWSYTDLLSWLNGYQKRYGFVYVDQDE
                     TQEGSLERYKKDSYYWYQKLIEENGENL"
     gene            1753..4200
                     /locus_tag="ACG842_002985"
     CDS             1753..4200
                     /locus_tag="ACG842_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HFQ0249405.1"
                     /translation="MAQKKRPTKKRKKTKKTKKQKQQQEQMLTIGVGFALILFAVFGF
                     LKLGFLGILIANGFRIIAGNTYQILCLLLAVLGFWIVIKNTEFSIGKNRRWFGGILFY
                     FGILLLLHAHLFGKLHTGEPNIMGTTWDLLASDIKQSQVDNNVGGGMIGAILYHFTYF
                     LIAQPGSYLAAVLLLAGGAFLFSNLEGYQLLNGIQSIGERVQELLEGDPAKQARKQAA
                     KEERMKQRAEAKEARRLAAKEAAEKEAAEYEKKKAVSQQRENTADEWQEPENTEPEQL
                     SFVPIDSFQENIHPANLEKPVPDTPKQTNTAEGFSDELPEDDGTSLEFEIEAEQENQD
                     YELPTVDLLDSIPTVDQSDEYKKIEKNIGVLEQTFQSFGVDAKVVKASLGPSVTKFEV
                     QPAVGVKVSKIVNLTDDIALALAAKDVRMEAPIPGKSLIGIEVPNGKISMVSFREIIE
                     AQPNHPDKLLEVPLGRDVSGRVQTADLSKMPHLLVAGSTGSGKSVAINGIITSILMRA
                     KPHEVKLMMIDPKMVELNMYNGIPHLLTPVVTNPRKAAQALQKVVQEMEERYEKFAAT
                     GVRNISGYNEFVQQKNLENGTKHPTLPFIVVIVDELADLMMVASNEVEDAIIRLAQMA
                     RAAGIHMILATQRPSVDVITGIIKANVPSRMAFAVSSGTDSRTIIDSNGAEKLLGRGD
                     MLFLPMGENKPIRVQGAFISDHEVERVVQFVTDQQEAHYEEKMMPTDEVETTGAPEQP
                     QDELFEEAKALVVEMQTASISLLQRRFRIGYNRAARLVDELEAHGVVGPSEGSKPRKV
                     FIEQEETSETLPDDNDM"
     gene            4430..5497
                     /gene="rlmN"
                     /locus_tag="ACG842_002986"
     CDS             4430..5497
                     /gene="rlmN"
                     /locus_tag="ACG842_002986"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA];
                     GO_process: GO:0070475 - rRNA base methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HFQ0249406.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            5487..5693
                     /locus_tag="ACG842_002987"
     CDS             5487..5693
                     /locus_tag="ACG842_002987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0249407.1"
                     /translation="MPNNRILLVFSITIKGAVFLIKELFNLNKRKDSIAKAIKLFLTR
                     KTLIKQRNVLNLMTACLMGVLAII"
     gene            complement(5690..6793)
                     /locus_tag="ACG842_002988"
     CDS             complement(5690..6793)
                     /locus_tag="ACG842_002988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HFQ0249408.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
     gene            7346..7894
                     /gene="aac(6')"
                     /locus_tag="ACG842_002989"
     CDS             7346..7894
                     /gene="aac(6')"
                     /locus_tag="ACG842_002989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HFQ0249409.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT         2697 a         1375 c         1751 g         2239 t
ORIGIN      
        1 tttcttacta ctcgaaacta agcgactttt tcacaacctc ctttacataa ttttattttg
       61 ttgtggaagg ggtttcgtgt tattctaaaa gaggaacgat tgaaggttat aggaaaggaa
      121 gagatgaatg gaacacaaag aacttacctc atttccaaaa gattttctgt ggggctctgc
      181 ttctgcagcg tatcaagtag aaggagcttg gcaagaagat gggaaaggag aatcagtctg
      241 ggatcgattt gtacggatac ctggaaagac atttaaagga acgaacggtg atttggcagt
      301 agaccactat catcgttata aagaagatat tgctttaatg aaagaacaag gtctgaaagc
      361 ttaccgcttc tctgtagctt ggacacggat ttttcctaat ggacgaggag aagtcaatca
      421 agctgggctc gctttttatg agagattgat cgacgaattg attgaaaacg atattgaacc
      481 tatgttgact ttatatcatt gggatcttcc acaagcattg caagatgaat ataatggttg
      541 ggaatcacgt cagatcattg aagatttcac tcattatgca gaaacattat ttgaggcatt
      601 ccgcgggaaa gtccattact ggattagttt gaatgaacaa aatatcttca cttcattagg
      661 ttatttgtta gcagcacatc cgccaggtgt gacagacccg aaacgaatgt atgaagttaa
      721 ccatattgct aatttagcaa atgcatctgt catcaataaa tttcacgagt tagaaatgcc
      781 tggaaaaatc ggaccaagct ttgcgtatac gccaaattat ccaattgaca gtaatcctga
      841 aaatgttctg gctgctgaaa atgcagaaga cctgatggcc aattattgga tggatgtcta
      901 tatgtgggga aaatatccga ttgctgccat gaggttctta gaagaaaaag gatgggcacc
      961 aacgattgaa gctggcgatg cagaattgct agaatctgct aaaccagatt ttcttgggat
     1021 caattactat caaacggcga cgaatgcatt caatccatta aacggagttg gtgctggaaa
     1081 aatgaatacg acaggaaaaa aaggttcatc agaagaaact ggagttcctg gaatgtataa
     1141 aaaagttgag aatccatttg tcgaacgaac aaattgggac tgggagattg acccagaagg
     1201 attaaggatc ggactgcgtc gtatcacaag ccgttaccgc atgccggtac tgattacgga
     1261 aaatggctta ggagagtatg acaagttgac agaagataaa caaatccatg acgactatcg
     1321 aatcaattac ttacaaagcc acgtcaaagc catcaaagaa gcaatttcgg atggagcaga
     1381 agtcctaggc tattgcacct ggtcttatac agatttgctt agctggctga acggttatca
     1441 aaagcgatat ggatttgtat atgttgatca agacgaaaca caagaaggat ctttggaacg
     1501 atataagaaa gacagttatt actggtacca aaaactgatt gaagaaaatg gagagaacct
     1561 ttagttcaca tacttaaaac aagaaggtac ggctggatat ggccgtgcct tttcttgata
     1621 aaatcattta gaaattaaga aaaactgtaa aataagctga aaaatgaaag aaagcgtcag
     1681 ttctttcaaa caaaacttta gttcccttac ttttttttgt ataatgaaac ttgatgaaat
     1741 gagggataat aaatggcaca aaagaaacgg cctactaaaa aaaggaaaaa gacgaaaaaa
     1801 acgaaaaagc aaaaacagca gcaggaacaa atgctgacga ttggtgttgg gtttgccttg
     1861 attttatttg ctgtcttcgg ctttttaaaa ttaggttttt taggaatatt gattgcaaac
     1921 ggctttcgga tcattgcagg aaatacttac caaatcctct gtcttttatt agccgtatta
     1981 ggattttgga ttgtcattaa gaatacagag ttttcaattg gtaaaaaccg acgctggttt
     2041 ggcggcatac tcttttattt cggtatctta ttattgttac atgcccattt atttggcaaa
     2101 ctccatactg gggaaccaaa tatcatgggg acgacatggg atttgttagc cagtgatata
     2161 aaacaaagtc aagtagacaa taacgttggt ggtgggatga ttggagcaat cctttatcat
     2221 tttacttact tcttgattgc acagcctgga agttatttag cagcagtgct attactggca
     2281 ggaggagcat tcctgttcag taatctggaa ggctatcagc tgttaaatgg tatccaatca
     2341 attggagaac gagtacagga attattggaa ggtgatcctg ccaagcaagc tcgaaagcaa
     2401 gcggcaaagg aagagagaat gaaacagcgc gcagaagcga aagaagcaag gcgcttagct
     2461 gcaaaagaag ctgcagaaaa agaagctgca gaatatgaga agaaaaaagc ggtgagccag
     2521 caaagggaaa atactgctga tgaatggcag gaaccagaaa atacagagcc ggagcagcta
     2581 agttttgttc caatcgacag ttttcaagaa aatatccatc ctgcgaatct agagaaacca
     2641 gtaccagata caccaaaaca gaccaataca gcagaaggtt tttcagatga gttgccagag
     2701 gatgacggta cctcattaga atttgagatt gaagcagaac aggaaaatca agactatgaa
     2761 ttaccgacag ttgatttgct tgattctata ccaactgtgg atcaaagtga tgagtataaa
     2821 aaaattgaaa aaaatatcgg cgtattagaa caaactttcc aaagtttcgg tgtcgatgcc
     2881 aaggtcgtca aagccagctt aggaccatcc gtcacaaaat tcgaagtcca gccggctgta
     2941 ggggtaaaag taagtaaaat tgtgaactta acagatgata tcgcgcttgc tttagcggcg
     3001 aaagatgtcc ggatggaagc accgattcca ggtaaatcac tgattggtat tgaagtacca
     3061 aatggaaaga tcagtatggt ttcctttaga gaaatcatcg aagcacaacc aaaccaccca
     3121 gataaattac tagaagtacc tttaggacgt gatgtttctg gacgggtaca aacggctgat
     3181 ctttcgaaga tgcctcactt actcgtggct ggttcgactg gtagtgggaa gtcagtagca
     3241 attaatggga ttatcacaag tatattaatg cgtgctaagc cccatgaagt aaaactgatg
     3301 atgatcgatc cgaaaatggt tgaattgaat atgtacaacg gcatacctca tctgttgacg
     3361 ccggtcgtga cgaaccctag aaaagcagcg caagccttac aaaaagtagt acaagaaatg
     3421 gaagaacgat atgaaaaatt cgcggcaacc ggagtaagaa atatttctgg ttacaatgaa
     3481 tttgttcaac agaaaaactt ggaaaacgga acgaaacatc caaccttacc attcatcgta
     3541 gtgatcgtag atgaattagc tgatttgatg atggtagcca gcaatgaagt tgaagatgcg
     3601 attattcgtt tagcgcaaat ggctcgggca gcggggattc atatgatcct agctactcaa
     3661 cgtccaagtg tggatgttat cacaggaatc atcaaagcaa atgtaccttc cagaatggca
     3721 tttgcggtat caagcggtac agattctcgg acgatcattg attctaatgg ggcagaaaag
     3781 ctcttagggc gaggagatat gctattcttg ccaatgggtg agaataaacc gatccgtgtg
     3841 caaggagcat tcatttctga ccacgaagta gaacgggtcg ttcaatttgt tacagatcag
     3901 caagaagctc attatgaaga gaaaatgatg ccgacagatg aagttgaaac gacaggtgct
     3961 ccagaacagc cacaagatga gctgtttgaa gaagcaaaag cacttgtcgt tgaaatgcaa
     4021 acggcaagta tttcccttct ccaaagaaga tttcggatcg gttacaaccg agcagcacgt
     4081 ttagtagatg aactggaagc tcatggagtt gttggcccgt cagaaggaag caagcctcgg
     4141 aaagtattca ttgagcagga agaaacatca gaaacgttgc cggacgataa tgacatgtaa
     4201 aacttgtgtg atgaagggtt gaaaaacgaa taaccaataa aaagacaagg catgatgata
     4261 ccaaaagaaa aaatggtttt tttccatctt ttctaatacg gtatgatgtc ttgtcttttt
     4321 agtttttgtg agattaaagt tctattcgat ttatttgtga agtattgcat taaggaaatg
     4381 aatcatgtat agtatgtagc gaaactttga gaaaaagagg gaaaatactt tggaaaaaca
     4441 gtccatttat ggcttgacga atgaagaatt aatcaattgg tttatagaaa acggcgagaa
     4501 aaaattccga gctgcccaag tttgggaatg gttgtatcaa aaacgtgtaa gtaatttcac
     4561 agaaatgacg aatctatcga aacaattgat cgaaaaatta tcggagcatt ttatcatcaa
     4621 tcctctaaaa cagatggtcg tccaagaagc ttcggatggt acagtaaaat atttgtttga
     4681 actccctgat aaaaacatga ttgagactgt actgatgaga caagaatatg gcttgtcagt
     4741 ttgtgtgacg acacaagtag gatgtaatat cggctgtacg ttttgtgcaa gcgggctgct
     4801 caaaaagaac cgtgatctga ctgctggaga aattgttgca cagatcatga tggtccaaca
     4861 ttattttgat gagcgcaatc ttggcgaacg cgtttcacat gtcgttgtaa tggggatcgg
     4921 tgagccattt gataattacg acaatgtaat ggactttttg catatcatca atgatgccaa
     4981 aggattggca attggtgctc ggcacattac agtttccact agtggattag cacacaaaat
     5041 caaggaattc gctaataacg gcttgcaagt caatctggct atttccttgc acgcgccgaa
     5101 taatgaggtg cgaacatcga tcatgcggat caaccgaagt ttcccaattg agaaattgat
     5161 ggaagctgtg gatgagtatt tagagaaaac aaaccgtcga atcacgtttg aatatatcat
     5221 gttgaatcaa gtaaatgata gaccagagca tgcgcaacaa ttagcagatc tattaaaaga
     5281 taagaaaaaa ttagcatatg tcaacttgat tccatataat ccagtcagtg aacatgatca
     5341 atattctcgc agtccaaaag ctgatgtatt gagattttat gatgtattga agaaaaacgg
     5401 cgtaaactgt gtgatacgta aagaacatgg tacagatatt gatgcagctt gcggacaatt
     5461 gcgaagcaag caaatgaaaa aaacagttgc caaataatcg tatcctactc gttttttcaa
     5521 ttaccataaa aggagcagtt ttcttaatta aggaactgtt caacttaaat aagagaaaag
     5581 attctatagc gaaagctata aagctttttt tgacaagaaa aaccctcatc aagcaaagaa
     5641 atgtcctcaa tttgatgaca gcctgcttga tgggggtttt agcaattatt taactgattt
     5701 tcttatatcc ttgctggctt tcttgtgtag catgataagt ttctacgtca agaatggcct
     5761 gtttaaattt ttctcgggtg atttcataag gcacatgttt aatatcattt gttgccatgg
     5821 ctttatctaa gattgcttct aaatcggtcg tgacattgag attcatgtcg gttaacgata
     5881 ctggaagttg gttctttttc ataaacacat agattttatc aaacaaatcg aactgctgat
     5941 ccatcatcaa taaggtcagt acaccataac tgacagatgc tccgtgaagg tatccctcca
     6001 ttttttctag aacagtcgta ccgtaataga atgagtgtgc catagcagaa ttatagttat
     6061 tgttaactaa aacagaaacc aatccggtag taacaatgat atccgaaacg acttgttcaa
     6121 ttgcatatga gggacgatcg tcggcactat ctttcaatgc ttgcgtacca taagtcaaga
     6181 gacgatcaat gcacattttt ccgatttgga ttcctaatct atccgtgtag cctagatcat
     6241 cgccacgagc agaaaaagta gattcgactt ctttggataa agcatcgcca atacctgccc
     6301 ataagtattt atcaggtgct tcggcaataa ttgctgtatc aatgaaggtg tgtacaggtg
     6361 gttttgaagg aaagaagagt ccgtccatta cgccttcttc gttatagatg acacataccg
     6421 cagtgacggg cgaacagttc gaaccaatag tgggaaaggt gaagactggt ttagctagtt
     6481 tttcaccaat gacttttaca gtatcgcaag cacggccacc accgactgca aacaggacgt
     6541 ctgctttttg gacactttca tgctggatca actgttcaat atttccataa gttgaattac
     6601 ccccgtacca gagactggcg acaacctcta tttcctgtgt caaagctact tttattttgt
     6661 tttctgcctt tgaaagtgca gtcttccctc caatcagcac tacttttgtc ccatattctt
     6721 tgacgattcc atttgctttt tcatacgcat ctgttccaat ggtataattt ggtaagaaaa
     6781 cactttgact catattcatt tccttctttc ttcgttgaaa cgggttaaaa ttttaaagag
     6841 tgatcgatct gactgcggct gtagatggag tagaggtgaa cctaagacag gataagtgat
     6901 gaataaggtg gcatgaacac cttgaaatca tcaataagta tcctaaaaac gaacaagtac
     6961 aaaaacattg aaaacgacaa taatgaattt ttgttcctct tagtatttct accgattccc
     7021 tttgcttttt tgtaattatc taaaaattct aaaaattaag attattatat gaaaacaggt
     7081 tttatttgtc aattcataat tggttttttt taaagcaaaa gataatctat atcatgatga
     7141 acaaacagag ttttttcctc caattgatgg ctttcctgat ctttcctatt ttgaataaag
     7201 tgtttctgta taattttttt attttataag taagataaat aatgaaagaa tagagatact
     7261 gattggtagc taaagatttt tgagcgagaa catttcgttt tttcaaaagg actgttagaa
     7321 taagcaagaa caagggggaa gacgtatgat aatcagtgaa tttgaccgta ataatccagt
     7381 attgaaagat cagctttctg atttactgag actgacttgg ccggaagaat atggagacag
     7441 ctcggcagaa gaagtagaag aaatgatgaa tccagaacga atcgcggtag cagcggtaga
     7501 ccaagatgag ttagtaggat ttattggtgc aatccctcaa tacggtatca caggttggga
     7561 attgcatcca ttagttgtag aaagctcccg acgaaagaac caaataggta ctcgattagt
     7621 caattactta gaaaaagaag tagcttccag aggaggaatc acgatttatt taggtacgga
     7681 tgatttagac catggaacaa cgttaagtca aacggacctg tatgagcata catttgataa
     7741 agtggcttcc atccagaacc ttcgtgaaca tccgtatgaa ttctatgaaa aattaggtta
     7801 taaaatcgta ggtgtcttac caaatgcaaa tggctgggac aaaccggata tttggatggc
     7861 aaaaacgatt attcctcgac caaattctca ataataaaaa gaggttgtga cagaagtaat
     7921 cagcttcaag aaataagaag gaatttacga aaattacttc ttgaattttt gtgaaattcc
     7981 aacttatttc cgaagaagct acttttggaa caccgtgtac cagaggttgt gaaaaagtcg
     8041 cttagtttcg agtagtaaga aa
//
LOCUS       DAZKLM010000273         4801 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3764
            SAMEA115706913-rid22582563.guided.140, whole genome shotgun
            sequence.
ACCESSION   DAZKLM010000273 DAZKLM010000000
VERSION     DAZKLM010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706913
            Sequence Read Archive: ERR13196647
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4801)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4801)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287823.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287823.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,004
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,849
            CDSs (with protein)               :: 2,849
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 41 of 96
            Pseudo Genes (incomplete)         :: 52 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 15 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4801
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706913-rid22582563.guided.140"
                     /isolate="VRE3764"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            89..784
                     /gene="vanR-A"
                     /locus_tag="ACG842_002990"
     CDS             89..784
                     /gene="vanR-A"
                     /locus_tag="ACG842_002990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance response regulator
                     transcription factor VanR-A"
                     /protein_id="HFQ0249410.1"
                     /translation="MSDKILIVDDEHEIADLVELYLKNENYTVFKYYTAKEALECIDK
                     SEIDLAILDIMLPGTSGLTICQKIRDKHTYPIIMLTGKDTEVDKITGLTIGADDYITK
                     PFRPLELIARVKAQLRRYKKFSGVKEQNENVIVHSGLVINVNTHECYLNEKQLSLTPT
                     EFSILRILCENKGNVVSSELLFHEIWGDEYFSKSNNTITVHIRHLREKMNDTIDNPKY
                     IKTVWGVGYKIEK"
     gene            762..1916
                     /locus_tag="ACG842_002991"
     CDS             762..1916
                     /locus_tag="ACG842_002991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance histidine kinase VanS-A"
                     /protein_id="HFQ0249411.1"
                     /translation="MVIKLKNKKNDYSKLERKLYMYIVAIVVVAIVFVLYIRSMIRGK
                     LGDWILSILENKYDLNHLDAMKLYQYSIRNNIDIFIYVAIVISILILCRVMLSKFAKY
                     FDEINTGIDVLIQNEDKQIELSAEMDVMEQKLNTLKRTLEKREQDAKLAEQRKNDVVM
                     YLAHDIKTPLTSIIGYLSLLDEAPDMPVDQKAKYVHITLDKAYRLEQLIDEFFEITRY
                     NLQTITLTKTHIDLYYMLVQMTDEFYPQLSAHGKQAVIHAPEDLTVSGDPDKLARVFN
                     NILKNAAAYSEDNSIIDITAGLSGDVVSIEFKNTGSIPKDKLAAIFEKFYRLDNARSS
                     DTGGAGLGLAIAKEIIVQHGGQIYAESNDNYTTFRVELPAMPDLVDKRRS"
     gene            2131..3099
                     /locus_tag="ACG842_002992"
     CDS             2131..3099
                     /locus_tag="ACG842_002992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-A"
                     /protein_id="HFQ0249412.1"
                     /translation="MNNIGITVYGCEQDEADAFHALSPRFGVMATIINANVSESNAKS
                     APFNQCISVGHKSEISASILLALKRAGVKYISTRSIGCNHIDTTAAKRMGITVDNVAY
                     SPDSVADYTMMLILMAVRNVKSIVRSVEKHDFRLDSDRGKVLSDMTVGVVGTGQIGKA
                     VIERLRGFGCKVLAYSRSRSIEVNYVPFDELLQNSDIVTLHVPLNTDTHYIISHEQIQ
                     RMKQGAFLINTGRGPLVDTYELVKALENGKLGGAALDVLEGEEEFFYSDCTQKPIDNQ
                     FLLKLQRMPNVIITPHTAYYTEQALRDTVEKTIKNCLDFERRQEHE"
     gene            3092..4123
                     /gene="vanA"
                     /locus_tag="ACG842_002993"
     CDS             3092..4123
                     /gene="vanA"
                     /locus_tag="ACG842_002993"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanA"
                     /protein_id="HFQ0249413.1"
                     /translation="MNRIKVAILFGGCSEEHDVSVKSAIEIAANINKEKYEPLYIGIT
                     KSGVWKMCEKPCAEWENDNCYSAVLSPDKKMHGLLVKKNHEYEINHVDVAFSALHGKS
                     GEDGSIQGLFELSGIPFVGCDIQSSAICMDKSLTYIVAKNAGIATPAFWVINKDDRPV
                     AATFTYPVFVKPARSGSSFGVKKVNSADELDYAIESARQYDSKILIEQAVSGCEVGCA
                     VLGNSAALVVGEVDQIRLQYGIFRIHQEVEPEKGSENAVITVPADLSAEERGRIQETA
                     KKIYKALGCRGLARVDMFLQDNGRIVLNEVNTLPGFTSYSRYPRMMAAAGIALPELID
                     RLIVLALKG"
     gene            4129..4737
                     /locus_tag="ACG842_002994"
     CDS             4129..4737
                     /locus_tag="ACG842_002994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-A"
                     /protein_id="HFQ0249414.1"
                     /translation="MEIGFTFLDEIVHGVRWDAKYATWDNFTGKPVDGYEVNRIVGTY
                     ELAESLLKAKELAATQGYGLLLWDGYRPKRAVNCFMQWAAQPENNLTKESYYPNIDRT
                     EMISKGYVASKSSHSRGSAIDLTLYRLDTGELVPMGSRFDFMDERSHHAANGISCNEA
                     QNRRRLRSIMENSGFEAYSLEWWHYVLRDEPYPNSYFDFPVK"
BASE COUNT         1481 a          921 c         1119 g         1280 t
ORIGIN      
        1 ggccttttat cttttacaca attatatgga ctttatccta taatctgata aattattaag
       61 ggcaaactta tgtgaaaggg tgataactat gagcgataaa atacttattg tggatgatga
      121 acatgaaatt gccgatttgg ttgaattata cttaaaaaac gagaattata cggttttcaa
      181 atactatacc gccaaagaag cattggaatg tatagacaag tctgagattg accttgccat
      241 attggacatc atgcttcccg gcacaagcgg ccttactatc tgtcaaaaaa taagggacaa
      301 gcacacctat ccgattatca tgctgaccgg gaaagataca gaggtagata aaattacagg
      361 gttaacaatc ggcgcggatg attatataac gaagcccttt cgcccactgg agttaattgc
      421 tcgggtaaag gcccagttgc gccgatacaa aaaattcagt ggagtaaagg agcagaacga
      481 aaatgttatc gtccactccg gccttgtcat taatgttaac acccatgagt gttatctgaa
      541 cgagaagcag ttatccctta ctcccaccga gttttcaata ctgcgaatcc tctgtgaaaa
      601 caaggggaat gtggttagct ccgagctgct atttcatgag atatggggcg acgaatattt
      661 cagcaagagc aacaacacca tcaccgtgca tatccggcat ttgcgcgaaa aaatgaacga
      721 caccattgat aatccgaaat atataaaaac ggtatggggg gttggttata aaattgaaaa
      781 ataaaaaaaa cgactattcc aaactagaac gaaaacttta catgtatatc gttgcaattg
      841 ttgtggtagc aattgtattc gtgttgtata ttcgttcaat gatccgaggg aaacttgggg
      901 attggatctt aagtattttg gaaaacaaat atgacttaaa tcacctggac gcgatgaaat
      961 tatatcaata ttccatacgg aacaatatag atatctttat ttatgtggcg attgtcatta
     1021 gtattcttat tctatgtcgc gtcatgcttt caaaattcgc aaaatacttt gacgagataa
     1081 ataccggcat tgatgtactt attcagaacg aagataaaca aattgagctt tctgcggaaa
     1141 tggatgttat ggaacaaaag ctcaacacat taaaacggac tctggaaaag cgagagcagg
     1201 atgcaaagct ggccgaacaa agaaaaaatg acgttgttat gtacttggcg cacgatatta
     1261 aaacgcccct tacatccatt atcggttatt tgagcctgct tgacgaggct ccagacatgc
     1321 cggtagatca aaaggcaaag tatgtgcata tcacgttgga caaagcgtat cgactcgaac
     1381 agctaatcga cgagtttttt gagattacac ggtataacct acaaacgata acgctaacaa
     1441 aaacgcacat agacctatac tatatgctgg tgcagatgac cgatgaattt tatcctcagc
     1501 tttccgcaca tggaaaacag gcggttattc acgcccccga ggatctgacc gtgtccggcg
     1561 accctgataa actcgcgaga gtctttaaca acattttgaa aaacgccgct gcatacagtg
     1621 aggataacag catcattgac attaccgcgg gcctctccgg ggatgtggtg tcaatcgaat
     1681 tcaagaacac tggaagcatc ccaaaagata agctagctgc catatttgaa aagttctata
     1741 ggctggacaa tgctcgttct tccgatacgg gtggcgcggg acttggattg gcgattgcaa
     1801 aagaaattat tgttcagcat ggagggcaga tttacgcgga aagcaatgat aactatacga
     1861 cgtttagggt agagcttcca gcgatgccag acttggttga taaaaggagg tcctaagaga
     1921 tgtatataat tttttaggaa aatctcaagg ttatctttac tttttcttag gaaattaaca
     1981 atttaatatt aagaaacggc tcgttcttac acggtagact taataccgta agaacgagcc
     2041 gttttcgttc ttcagagaaa gatttgacaa gattaccatt ggcatccccg ttttatttgg
     2101 tgcctttcac agaaagggtt ggtcttaatt atgaataaca tcggcattac tgtttatgga
     2161 tgtgagcagg atgaggcaga tgcattccat gctctttcgc ctcgctttgg cgttatggca
     2221 acgataatta acgccaacgt gtcggaatcc aacgccaaat ccgcgccttt caatcaatgt
     2281 atcagtgtgg gacataaatc agagatttcc gcctctattc ttcttgcgct gaagagagcc
     2341 ggtgtgaaat atatttctac ccgaagcatc ggctgcaatc atatagatac aactgctgct
     2401 aagagaatgg gcatcactgt cgacaatgtg gcgtactcgc cggatagcgt tgccgattat
     2461 actatgatgc taattcttat ggcagtacgc aacgtaaaat cgattgtgcg ctctgtggaa
     2521 aaacatgatt tcaggttgga cagcgaccgt ggcaaggtac tcagcgacat gacagttggt
     2581 gtggtgggaa cgggccagat aggcaaagcg gttattgagc ggctgcgagg atttggatgt
     2641 aaagtgttgg cttatagtcg cagccgaagt atagaggtaa actatgtacc gtttgatgag
     2701 ttgctgcaaa atagcgatat cgttacgctt catgtgccgc tcaatacgga tacgcactat
     2761 attatcagcc acgaacaaat acagagaatg aagcaaggag catttcttat caatactggg
     2821 cgcggtccac ttgtagatac ctatgagttg gttaaagcat tagaaaacgg gaaactgggc
     2881 ggtgccgcat tggatgtatt ggaaggagag gaagagtttt tctactctga ttgcacccaa
     2941 aaaccaattg ataatcaatt tttacttaaa cttcaaagaa tgcctaacgt gataatcaca
     3001 ccgcatacgg cctattatac cgagcaagcg ttgcgtgata ccgttgaaaa aaccattaaa
     3061 aactgtttgg attttgaaag gagacaggag catgaataga ataaaagttg caatactgtt
     3121 tgggggttgc tcagaggagc atgacgtatc ggtaaaatct gcaatagaga tagccgctaa
     3181 cattaataaa gaaaaatacg agccgttata cattggaatt acgaaatctg gtgtatggaa
     3241 aatgtgcgaa aaaccttgcg cggaatggga aaacgacaat tgctattcag ctgtactctc
     3301 gccggataaa aaaatgcacg gattacttgt taaaaagaac catgaatatg aaatcaacca
     3361 tgttgatgta gcattttcag ctttgcatgg caagtcaggt gaagatggat ccatacaagg
     3421 tctgtttgaa ttgtccggta tcccttttgt aggctgcgat attcaaagct cagcaatttg
     3481 tatggacaaa tcgttgacat acatcgttgc gaaaaatgct gggatagcta ctcccgcctt
     3541 ttgggttatt aataaagatg ataggccggt ggcagctacg tttacctatc ctgtttttgt
     3601 taagccggcg cgttcaggct catccttcgg tgtgaaaaaa gtcaatagcg cggacgaatt
     3661 ggactacgca attgaatcgg caagacaata tgacagcaaa atcttaattg agcaggctgt
     3721 ttcgggctgt gaggtcggtt gtgcggtatt gggaaacagt gccgcgttag ttgttggcga
     3781 ggtggaccaa atcaggctgc agtacggaat ctttcgtatt catcaggaag tcgagccgga
     3841 aaaaggctct gaaaacgcag ttataaccgt tcccgcagac ctttcagcag aggagcgagg
     3901 acggatacag gaaacggcaa aaaaaatata taaagcgctc ggctgtagag gtctagcccg
     3961 tgtggatatg tttttacaag ataacggccg cattgtactg aacgaagtca atactctgcc
     4021 cggtttcacg tcatacagtc gttatccccg tatgatggcc gctgcaggta ttgcacttcc
     4081 cgaactgatt gaccgcttga tcgtattagc gttaaagggg tgataagcat ggaaatagga
     4141 tttacttttt tagatgaaat agtacacggt gttcgttggg acgctaaata tgccacttgg
     4201 gataatttca ccggaaaacc ggttgacggt tatgaagtaa atcgcattgt agggacatac
     4261 gagttggctg aatcgctttt gaaggcaaaa gaactggctg ctacccaagg gtacggattg
     4321 cttctatggg acggttaccg tcctaagcgt gctgtaaact gttttatgca atgggctgca
     4381 cagccggaaa ataacctgac aaaggaaagt tattatccca atattgaccg aactgagatg
     4441 atttcaaaag gatacgtggc ttcaaaatca agccatagcc gcggcagtgc cattgatctt
     4501 acgctttatc gattagacac gggtgagctt gtaccaatgg ggagccgatt tgattttatg
     4561 gatgaacgct ctcatcatgc ggcaaatgga atatcatgca atgaagcgca aaatcgcaga
     4621 cgtttgcgct ccatcatgga aaacagtggg tttgaagcat atagcctcga atggtggcac
     4681 tatgtattaa gagacgaacc ataccccaat agctattttg atttccccgt taaataaact
     4741 tttaaccgtt gcacggacaa acggttctgt tgcaaagttt taaataaaga ataaaatccc
     4801 t
//
LOCUS       DAZKLM010000274         3163 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3764
            SAMEA115706913-rid22582563.guided.162, whole genome shotgun
            sequence.
ACCESSION   DAZKLM010000274 DAZKLM010000000
VERSION     DAZKLM010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706913
            Sequence Read Archive: ERR13196647
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3163)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3163)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287823.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287823.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,004
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,849
            CDSs (with protein)               :: 2,849
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 41 of 96
            Pseudo Genes (incomplete)         :: 52 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 15 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3163
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706913-rid22582563.guided.162"
                     /isolate="VRE3764"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            218..715
                     /gene="dfrG"
                     /locus_tag="ACG842_002995"
     CDS             218..715
                     /gene="dfrG"
                     /locus_tag="ACG842_002995"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HFQ0249415.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
     gene            complement(787..2739)
                     /locus_tag="ACG842_002996"
     CDS             complement(787..2739)
                     /locus_tag="ACG842_002996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HFQ0249416.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
     gene            complement(2746..2982)
                     /locus_tag="ACG842_002997"
     CDS             complement(2746..2982)
                     /locus_tag="ACG842_002997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001159903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0249417.1"
                     /translation="MQFLEPKNKNAKSVDWEISEQVRVIVKQYAEYAERTESEAVDEF
                     LLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
BASE COUNT          977 a          548 c          523 g         1115 t
ORIGIN      
        1 ttttcatttt aacttacata ccaaattaca tacaaaatga tttataaaat gcaattgaaa
       61 atgataattg aatgaaaatt tgtgattttg tggtattatg ataatataac ttatagaaaa
      121 aattgcttat tcaactaacg ggcaggataa tgagaaaagg ttttttgata gaaataagaa
      181 attagatggt aaattagtta aaaaggctgt gttataaatg aaagtttctt tgattgctgc
      241 gatggataag aatagagtga ttggcaaaga gaatgacatt ccttggagga ttcccaagga
      301 ctgggaatat gttaaaaata ctacaaaggg acatccgata atattaggta ggaagaacct
      361 tgaatcaatc ggaagagcct tacctgacag aagaaatatt attctgacga gagataaggg
      421 gtttaccttt aatggttgtg aaattgttca ttcaatagaa gatgtttttg agttatgtaa
      481 aaacgaagaa gaaattttta ttttcggagg agaacagatt tataatttgt ttttccctta
      541 tgttgagaaa atgtacatca caaaaataca tcatgaattc gaaggagata ctttttttcc
      601 agaagtgaat tatgaggaat ggaatgaggt atttgcccaa aaagggataa agaatgataa
      661 aaatccgtat aactactatt ttcatgtata tgaaagaaaa aacttattga gttaacgggt
      721 gcgttaattg aagaagaaca gtaaagataa agcacaagag cttgggatta ctccaagctc
      781 tttttgtcat ttgaaataaa ttatatcttt ccaatcaaat tgtttatctg taatccctaa
      841 tctttgagca ggtgtcaaaa cctttttatc cgctgattta aaagggcgac aaaagttgta
      901 gtaagttctt agaattgtaa tcgcaaattg agcatacttc ggattgaagt tagcgtaaat
      961 atagctcttc ttatcaccac gagctgttgt taatggtctt tcaagagatg atatataacg
     1021 tcttatctgt tgaataaagc tattggtcga atggtcatta acatttaata gcatctttgc
     1081 gatttcgttt ggttccagag cagataaatc cgttctacaa tcaactgaat aaaatccttt
     1141 atccttagtg gctaatggat gtttgatagg gttatcggca tatttgcgat atctttctcc
     1201 atctttacta atagcctctt cgtggaattg atgacttgtt agtaattcag ttagataatg
     1261 agatgccaaa gtccgtagaa actttgtttt gaatcctctg atatctcccc aatcgagcag
     1321 ttctgctttt gcttgtccga attcttttaa gcattgtttt ctgcttttcg tcttgttaac
     1381 ttggcaaata aaatgatgtg catcgctcaa ccttaattct ttcgtgaata ctctataaaa
     1441 ggcgtttcta atagagaagt catcatcact aatcattcgc cattcgtcgg cattaaccat
     1501 ttgtttaatt aaccaatagt gagcagttgt agtataagga gcatcaacat ttaaaccatc
     1561 gacaaatcta ctcctattag taattttccc taattcgtga agataagcat tcctattttc
     1621 gctatcgtta gcggatggtt cttgtggata ataagaccaa tctaacctat catttttacg
     1681 actaaaagta ttgagatggt cttctttgaa ttttcttgta tcttcattta attcatccat
     1741 tgaaatattc caatcatagg caacatcaga acggaacaca tatcttgata agacctcagc
     1801 agatacaact atatacgttt gcatattaag gtcttcaaac cccgtatatt tctttgaacc
     1861 ttgtcctttc ttacggacgt tatttaggta gtagtgcatc ttgtccgtat taagccacat
     1921 ctcgttaaat ttctttgttt gaaggggttg tgtctcatat cgttctaaaa actccaaaca
     1981 acgcctatac agccactcca gcttatgata gtaggttcca actccaattc ctaaaatatc
     2041 acaagtacga ctgactgaaa cccttccaac aaccatctta gcgaacattg gaagaatggt
     2101 gttcttttgt tggtgataag tagtggtttc ttctcttttt ggtagaacat tagtgaactt
     2161 cttacacgcc ttacattgat agcgttgaga tttgccccta cttttccccc ttttgtaaaa
     2221 ttgcttcggc tcattgaatg gggttgattc ttcttccgag cagccttctt tatgaaattg
     2281 ataatctggc tcaacatctt ggatgctatt gatttctata aggcgtttaa tttcttcaac
     2341 aacagaccaa ttagataaag gggttactgt acagttttgc gatacacctc tgccaatagg
     2401 attgtcattg cagtacatac ctttatgacc tttatctttg gaactaccag tcattttata
     2461 tcgacttggt ttccctttta catctttgaa tttgtgctgt tctttcccaa aattacaaca
     2521 aaacggattg atgcagtaat tatattgaat gggatattca gaaccgttcc atttaaaaga
     2581 cgttggcaag aacaacattt tgttatacct tgttgcaaat cgttttggag ttaataaatc
     2641 atattgttta gccctatctt ttaattcttc gtttgaaatc ggaattttaa catctacaat
     2701 ttcatcttcc ttcgtggcta atcttttaag cttggtcatt gtctatcaac ccactctatc
     2761 tttgataccc attttttcaa caatccgctt attacttcgc ttgttggcaa tccattcaat
     2821 aaacttctta tcgtctaaaa tgttcaataa gaattcatcc actgcttcgc tttcagtacg
     2881 ctctgcatat tcagcgtatt gcttaactat cacccttact tgctctgata tttcccaatc
     2941 cacagactta gcgttcttat tttttggctc aagaaattgc ataggaaaat gcaccccttt
     3001 ttgcttagta aattgtaacc attttaacac accaatttgc attcctacaa taattatttc
     3061 acgttcgcat ttttatatac attttaactt acattttgtg gtgtaaatta acaacaaacg
     3121 ttgatttaac aataaaaaac gagtatcaaa ttcagttgca att
//
LOCUS       DAZKLM010000275         2189 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3764
            SAMEA115706913-rid22582563.guided.179, whole genome shotgun
            sequence.
ACCESSION   DAZKLM010000275 DAZKLM010000000
VERSION     DAZKLM010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706913
            Sequence Read Archive: ERR13196647
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2189)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2189)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287823.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287823.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,004
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,849
            CDSs (with protein)               :: 2,849
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 41 of 96
            Pseudo Genes (incomplete)         :: 52 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 15 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2189
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706913-rid22582563.guided.179"
                     /isolate="VRE3764"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..698
                     /locus_tag="ACG842_002998"
     CDS             <1..698
                     /locus_tag="ACG842_002998"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008425 -
                     2-polyprenyl-6-methoxy-1,4-benzoquinone methyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0061542 - 3-demethylubiquinol-n
                     3-O-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0102208 - 2-polyprenyl-6-hydroxyphenol
                     methylase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFQ0249418.1"
                     /translation="KYSQMSRSQKGLAGAGEWETLKKMLPDFKGKRVLDLGCGYGWHC
                     IYAMENGASSVVGVDISHKMLEVAKGKTHFPQIEYECCAIEDVDFPEESFDVILSSLA
                     FHYVADYENLIKKIYRMLKAGGNLVFTVEHPVFTAHGTQDWYYNEKGEILHFPVDNYY
                     YEGKRTAMFLEEKVTKYHRTLTTYLNTLLSNSFIINQIVEPQPPENMMDIPGMADEMR
                     RPMMLIVSAKKKM"
     gene            731..1639
                     /locus_tag="ACG842_002999"
     CDS             731..1639
                     /locus_tag="ACG842_002999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase ANT(6)-Ia"
                     /protein_id="HFQ0249419.1"
                     /translation="MRSEKEMMDLVLSLAEQDERIRIVTLEGSRANINIPKDEFQDYD
                     ITYFVSDIEPFISNDDWLNQFGNIIMMQKPEDMELFPPEEKGFSYLMLFDDYNKIDLT
                     LLPLEELDNYLKGDKLIKVLIDKDCRIKRDIVPTDIDYHVRKPSAREYDDCCNEFWNV
                     TPYVIKGLCRKEILFAIDHFNQIVRHELLRMISWKVGIETGFKLSVGKNYKFIERYIS
                     EDLWEKLLSTYRMDSYENIWEALFLCHQLFRAVSGEVAERLHYAYPEYDRNITKYTRD
                     MYKKYTGKTGCLDSTYAADIEERREQ"
     gene            1636..2116
                     /locus_tag="ACG842_003000"
                     /pseudo
     CDS             1636..2116
                     /locus_tag="ACG842_003000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
BASE COUNT          757 a          316 c          497 g          619 t
ORIGIN      
        1 aaaaatacag tcaaatgagt cgctcgcaga aaggactggc tggtgcggga gaatgggaga
       61 ctttgaaaaa gatgctacct gattttaagg gtaagcgtgt gcttgattta ggatgcggct
      121 atggatggca ctgtatatat gcgatggaaa acggtgcttc ctctgtagta ggtgttgata
      181 tttctcataa aatgctcgaa gtagcaaaag gaaaaaccca ttttccacag attgaatatg
      241 aatgctgtgc catagaagat gtggatttcc cagaggagag ctttgatgta atactaagtt
      301 cgcttgcgtt tcattatgta gcagactatg agaatttaat aaaaaagata tataggatgc
      361 tgaaggctgg tggcaattta gtttttacag ttgaacatcc tgtttttact gctcatggaa
      421 cacaagactg gtattataac gaaaaaggag aaatactgca tttcccggtg gacaattatt
      481 attatgaggg caaacggaca gctatgtttt tggaagaaaa ggttacaaaa tatcatagaa
      541 cactgaccac atatctaaat acactgcttt caaatagttt tataataaat cagattgtgg
      601 agccacagcc gccagagaac atgatggata ttccggggat ggcggatgaa atgcgacgcc
      661 caatgatgct gattgtatcg gcaaaaaaga agatgtaata atatagaaaa aataaacgag
      721 gagtatgtaa atgagatcag aaaaagaaat gatggattta gtactttctt tagcagaaca
      781 ggatgaacgt attcgaattg tgacccttga ggggtcacgc gcaaatatta atatacctaa
      841 agatgaattt caggattatg atattacata ttttgtaagt gatatagaac cgtttatatc
      901 taatgatgac tggcttaatc aatttgggaa tataataatg atgcaaaagc cggaggatat
      961 ggaattattc ccacctgaag aaaagggatt ttcctatctt atgctatttg atgattacaa
     1021 taaaattgat cttaccttat tgcccttgga agagttagat aattacctaa agggcgataa
     1081 attaataaag gttctaattg ataaagattg tagaattaaa agggacatag ttccgactga
     1141 tatagattat catgtaagaa agccaagcgc aagggagtat gatgattgct gcaatgaatt
     1201 ttggaatgta acaccttatg ttattaaagg attgtgccgt aaggaaattt tatttgctat
     1261 tgatcatttt aatcagattg ttcgccatga gctgctgaga atgatatcat ggaaggtcgg
     1321 catcgaaaca ggctttaaat taagtgtagg caagaactat aagtttattg aaaggtatat
     1381 atccgaggat ttgtgggaga aacttttgtc cacctaccgg atggattcct atgaaaacat
     1441 atgggaagca ttatttctat gccatcaatt gttcagggcg gtatccggtg aggtggcgga
     1501 aaggcttcat tatgcctatc cggagtatga taggaatata acaaaatata ccagggacat
     1561 gtataaaaaa tacactggta aaaccggctg cctggatagc acatatgccg ctgatataga
     1621 agagaggcgg gaacagtgat tacagaaatg aaagcagggc acctgaaaga tatcgataaa
     1681 cccagcgaac catttgaggt gataggtaag attataccga ggtatgaaaa cgagaattgg
     1741 acctttacag aattactcta tgaagcgcca tatttaaaaa gctaccaaga cgaagaggat
     1801 gaagaggatg aggaggcaga ttgccttgaa tatattgaca atactgataa gataatatat
     1861 cttttatata gaagatatcg ccgtatgtaa ggatttcagg gggcaaggca taggcagcgc
     1921 gcttatcaat atatctatag aatgggcaaa gcataaaaac ttgcatggac taatgcttga
     1981 aacccaggac aataacctta tagcttgtaa attctatcat aattgtggtt tcaaaatcgg
     2041 ctccgtcgat actatgttat acgccaactt tgaaaacaac tttgaaaaag ctgttttctg
     2101 gtatttaagg ttttagaatg caaggaacag tgaattggag ttcgtcttgt ggttctgttg
     2161 caaagtttta aataaagaat aaaatccct
//
LOCUS       DAZKLM010000276         1184 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3764
            SAMEA115706913-rid22582563.guided.218, whole genome shotgun
            sequence.
ACCESSION   DAZKLM010000276 DAZKLM010000000
VERSION     DAZKLM010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706913
            Sequence Read Archive: ERR13196647
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1184)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1184)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287823.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287823.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,004
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,849
            CDSs (with protein)               :: 2,849
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 41 of 96
            Pseudo Genes (incomplete)         :: 52 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 15 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1184
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706913-rid22582563.guided.218"
                     /isolate="VRE3764"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            54..403
                     /locus_tag="ACG842_003001"
                     /pseudo
     CDS             54..403
                     /locus_tag="ACG842_003001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741963.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
     gene            complement(675..965)
                     /locus_tag="ACG842_003002"
     CDS             complement(675..965)
                     /locus_tag="ACG842_003002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0249420.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
BASE COUNT          373 a          231 c          180 g          400 t
ORIGIN      
        1 tagctttaag cattacttaa agataactta aaagttatct tttaaatact taattgttaa
       61 ataccttatt tgatgcaaaa gaaatctacg atagtcaaca aaaaaatttt cattatatcc
      121 agaaaaattt ttcttagtta aggacctatg gattgcagta atgcagaatt cgtcgaataa
      181 actgccaaag acatacaatc atattgcttt taaaattgac gaacaagata tagattcgtt
      241 tgtctcaaaa atccaaatgt taggtttaac tgtcgaacca ggacgttctc gtgtgaaagg
      301 cgaggcttct tcaatttact tttacgacta tgataatcac ctcttcgagt tacataccgg
      361 tactctccag gaacgtttaa aatcctacaa ttccactacc tgagactagt aatgactagt
      421 ctctttttat ggcctctgat ccccccattg agcgaatact aatagggctt cgaattgatc
      481 tgttcaatct ttaagcaata tccctcaaat gagtttggaa agtttagtga ttcatcacaa
      541 atacttaaat acatgtagtt atatatagat tgaggttctt gtgagcgtat gctcgtgtgg
      601 gttcaaatcc catcgtctgc atccatcttt ccaaaacaag cggctaggac atttttgtct
      661 cagccgcttg ttttctagtt tattctttca tcttgtcgga ccttttttct tgggtataga
      721 cttttctcat attctaccac aattttgggt ttccctaaca catgatagga tctcacaaca
      781 aattgctttc tattattata ataacctgta acgactaact tcatcccttc gtctacttca
      841 gctaggaagt tcaaactatg ggaagcaatc aaacaattga ccccatttaa actgaaacgg
      901 atcaacggtc tggcactaac ttgcaaagta cggatcttat ctactgttcc atattttttc
      961 tccataacct tcacctcaat accattatac gaacatacat tccctttgta aagaagaaac
     1021 tctgttttat ctttttgact ttttgtctat tacctttctt aaaatcatat tggcacttcc
     1081 tgcgaagatg aaggacatgt actaaaaaaa agaggcagca gtatagattt aagtagttca
     1141 gttttggagt acaaaaagtg aaaatgtaaa cctaaatgag tatc
//
LOCUS       DAZKLM010000277         1145 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3764
            SAMEA115706913-rid22582563.guided.220, whole genome shotgun
            sequence.
ACCESSION   DAZKLM010000277 DAZKLM010000000
VERSION     DAZKLM010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706913
            Sequence Read Archive: ERR13196647
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1145)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1145)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287823.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287823.1
            Genome Coverage       :: 210x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:29:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,004
            CDSs (total)                      :: 2,945
            Genes (coding)                    :: 2,849
            CDSs (with protein)               :: 2,849
            Genes (RNA)                       :: 59
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 41 of 96
            Pseudo Genes (incomplete)         :: 52 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 15 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1145
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706913-rid22582563.guided.220"
                     /isolate="VRE3764"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            23..73
                     /locus_tag="ACG842_003003"
     CDS             23..73
                     /locus_tag="ACG842_003003"
                     /inference="COORDINATES: protein motif:HMM:NF018061.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuation leader
                     peptide"
                     /protein_id="HFQ0249421.1"
                     /translation="MWTLSKQTKNSDYADK"
     gene            198..935
                     /gene="erm(B)"
                     /locus_tag="ACG842_003004"
     CDS             198..935
                     /gene="erm(B)"
                     /locus_tag="ACG842_003004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011100845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HFQ0249422.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESRASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTITYEQVLSIFNSYLLFNGRK"
BASE COUNT          420 a          199 c          184 g          342 t
ORIGIN      
        1 gccttttatc ttttacacaa ttatatggac tttatcgaaa cagactaaaa acagtgatta
       61 cgcagataaa taaatacgtt agattaattc ctaccagtga ctaatcttat gactttttaa
      121 acagataact aaaattacaa acaaatcgtt taacttctgt atttgtttat agatgtaatc
      181 acttcaggag agattacatg aacaaaaata taaaatattc tcaaaacttt ttaacgagtg
      241 aaaaagtact caaccaaata ataaaacaat tgaatttaaa agaaaccgat accgtttacg
      301 aaattggaac aggtaaaggg catttaacga cgaaactggc taaaataagt aaacaggtaa
      361 cgtctattga attagacagt catctattca acttatcgtc agaaaaatta aaactgaata
      421 ctcgtgtcac tttaattcac caagatattc tacagtttca attccctaac aaacagaggt
      481 ataaaattgt tgggaatatt ccttaccatt taagcacaca aattattaaa aaagtggttt
      541 ttgaaagccg tgcgtctgac atctatctga ttgttgaaga aggattctac aagcgtacct
      601 tggatattca ccgaacacta gggttgctct tgcacactca agtctcgatt cagcaattgc
      661 ttaagctgcc agcggaatgc tttcatccta aaccaaaagt aaacagtgtc ttaataaaac
      721 ttacccgcca taccacagat gttccagata aatattggaa gctatatacg tactttgttt
      781 caaaatgggt caatcgagaa tatcgtcaac tgtttactaa aaatcagttt catcaagcaa
      841 tgaaacacgc caaagtaaac aatttaagta ccattactta tgagcaagta ttgtctattt
      901 ttaatagtta tctattattt aacgggagga aataattcta tgagtcgctt ttttaaattt
      961 ggaaagttac acgttactaa agggaatgga gataaattat tagatatact actgacagct
     1021 tccaagaagc taaagaggtc cctagcgcct acggggaatt tgtatcgata aggggtacaa
     1081 attcccacta agcgctcggg accccttgta ggaaaatgtc ctaagtgtgg caacaatatt
     1141 gtatt
//
